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Sample records for normal hematological parameters

  1. Comprehensive reference ranges for hematology and clinical chemistry laboratory parameters derived from normal Nigerian adults.

    Science.gov (United States)

    Miri-Dashe, Timzing; Osawe, Sophia; Tokdung, Monday; Daniel, Monday Tokdung Nenbammun; Daniel, Nenbammun; Choji, Rahila Pam; Mamman, Ille; Deme, Kurt; Damulak, Dapus; Abimiku, Alash'le

    2014-01-01

    Interpretation of laboratory test results with appropriate diagnostic accuracy requires reference or cutoff values. This study is a comprehensive determination of reference values for hematology and clinical chemistry in apparently healthy voluntary non-remunerated blood donors and pregnant women. Consented clients were clinically screened and counseled before testing for HIV, Hepatitis B, Hepatitis C and Syphilis. Standard national blood donors' questionnaire was administered to consented blood donors. Blood from qualified volunteers was used for measurement of complete hematology and chemistry parameters. Blood samples were analyzed from a total of 383 participants, 124 (32.4%) males, 125 (32.6%) non-pregnant females and 134 pregnant females (35.2%) with a mean age of 31 years. Our results showed that the red blood cells count (RBC), Hemoglobin (HB) and Hematocrit (HCT) had significant gender difference (p = 0.000) but not for total white blood count (p>0.05) which was only significantly higher in pregnant verses non-pregnant women (p = 0.000). Hemoglobin and Hematocrit values were lower in pregnancy (P = 0.000). Platelets were significantly higher in females than men (p = 0.001) but lower in pregnant women (p =  .001) with marked difference in gestational period. For clinical chemistry parameters, there was no significant difference for sodium, potassium and chloride (p>0.05) but gender difference exists for Bicarbonate (HCO3), Urea nitrogen, Creatinine as well as the lipids (p0.05). Hematological and Clinical Chemistry reference ranges established in this study showed significant gender differences. Pregnant women also differed from non-pregnant females and during pregnancy. This is the first of such comprehensive study to establish reference values among adult Nigerians and difference observed underscore the need to establish reference values for different populations.

  2. Comprehensive reference ranges for hematology and clinical chemistry laboratory parameters derived from normal Nigerian adults.

    Directory of Open Access Journals (Sweden)

    Timzing Miri-Dashe

    Full Text Available BACKGROUND: Interpretation of laboratory test results with appropriate diagnostic accuracy requires reference or cutoff values. This study is a comprehensive determination of reference values for hematology and clinical chemistry in apparently healthy voluntary non-remunerated blood donors and pregnant women. METHODS AND FINDINGS: Consented clients were clinically screened and counseled before testing for HIV, Hepatitis B, Hepatitis C and Syphilis. Standard national blood donors' questionnaire was administered to consented blood donors. Blood from qualified volunteers was used for measurement of complete hematology and chemistry parameters. Blood samples were analyzed from a total of 383 participants, 124 (32.4% males, 125 (32.6% non-pregnant females and 134 pregnant females (35.2% with a mean age of 31 years. Our results showed that the red blood cells count (RBC, Hemoglobin (HB and Hematocrit (HCT had significant gender difference (p = 0.000 but not for total white blood count (p>0.05 which was only significantly higher in pregnant verses non-pregnant women (p = 0.000. Hemoglobin and Hematocrit values were lower in pregnancy (P = 0.000. Platelets were significantly higher in females than men (p = 0.001 but lower in pregnant women (p =  .001 with marked difference in gestational period. For clinical chemistry parameters, there was no significant difference for sodium, potassium and chloride (p>0.05 but gender difference exists for Bicarbonate (HCO3, Urea nitrogen, Creatinine as well as the lipids (p0.05. CONCLUSIONS: Hematological and Clinical Chemistry reference ranges established in this study showed significant gender differences. Pregnant women also differed from non-pregnant females and during pregnancy. This is the first of such comprehensive study to establish reference values among adult Nigerians and difference observed underscore the need to establish reference values for different populations.

  3. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and basophiles counts were ...

  4. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    AJB SERVER

    2007-01-04

    Jan 4, 2007 ... The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and.

  5. Effect of electroconvulsive therapy on hematological parameters.

    Science.gov (United States)

    Chaturvedi, S; Chadda, R K; Rusia, U; Jain, N

    2001-11-30

    Although a complete blood count is part of the evaluation before the use of electroconvulsive therapy (ECT), there are no known hematological contraindications for the procedure. A preliminary study was done on 31 randomly selected psychiatric patients (chronic schizophrenia, n=10; acute depression, n=8; acute mania, n=6; acute psychosis, n=6; delusional disorder, n=1) receiving ECT to study its hematological effects. Blood samples were drawn just before and 0, 1 and 2 h after ECT. Hemoglobin (Hb%), total and differential leukocyte count (TLC and DLC), red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and platelet count were measured on a fully automated hematology analyzer (Sysmex K-1000). Significant changes were found in TLC, percentage of polymorphs and lymphocytes, and Hb%. Changes in other parameters were not statistically significant. More such studies are needed to substantiate these observations and to understand the mechanism and implication of these effects.

  6. Hematological parameters after acute radiation injury

    International Nuclear Information System (INIS)

    Hirashima, Kunitake

    1989-01-01

    According to clinical experiences of radiation accidents during the past two decades, utilization of measured hematologic changes as a direcrt indicator of the severity of radiation injury provides important information for diagnosis and prognostic evaluation in individual cases. Hematologic changes can be described in terms of prognostic categories based on the possible outcome of the acute radiation syndrome. The five categories suggested by Wald according to the grade of severity. By the actual application of this category to our experience of the 1971 Chiba accident of exposure to irridium 192, it was proved that the estimated dose was well correlated to the value by cytogenetic analysis and physical estimation used of thermo-luminescence phenomena. In hematological parameters, a decrease of lymphocytes occurs whithin 24 hours after the exposure. The level of this early lymphopenia is regarded as one of the best indicators of severity of radiation injury. For the decision of therapeutic procedures, however, the total granulocyte count and platelet count are more valuable to exclude severe infection and bleeding symptoms occurred one month after the exposure. The limitation of the approach by hematologic data must exist in the case exposed in a non-uniform fashion. To overwhelm this difficulty, the application of rapid marrow scanning by short-lived RI such as 52 Fe is expected and the bone marrow imaging by magnetic resonance studies is more exciting. For more sensitive and technically easy-drived methods detecting hematologic injury, our new method of detecting micro-nucleus in polychromatic erythroblasts from cultured erythroid colonies from peripheral blood is now developing. Preliminary data have shown the sensitivity of this method is comparable to the cytogenetic study of pheripheral lymphocytes. (author)

  7. Hematological parameters in children with Down syndrome

    Directory of Open Access Journals (Sweden)

    Renato Nisihara

    2015-04-01

    Full Text Available Introduction: There are few studies that investigated whether Down syndrome (DS interferes with references values for complete blood counts (CBC test in children with the syndrome. Objective: This study aimed to analyze the results of CBC performed in children with DS. Patients and methods: Data from CBC of DS children were included; at the time of examination they were aged between 2 and 10 years and had no clinical signs and/or symptoms of infectious disease. The hematological parameters analyzed were: total number of erythrocytes (RBC, hemoglobin (Hb concentration, hematological indices, platelet count, and total number of leucocytes. Additionally, we compared the collected parameters according to gender and age of the children studied. Results: A total of 203 CBC (100 girls and 103 boys were evaluated. In general, no significant differences were observed in studied parameters between the values found in samples of DS children and the values described in the literature as a reference for children in this age group. No difference in the prevalence of anemia was observed in relation to gender (p = 0.33, 14/103 (13.6% boys, and 11/100 (11% girls had anemia. However, the Hb and hematological indices values found in boys was significantly lower than in girls (p < 0.001. Conclusion: This investigation is the first one in Brazil to present and analyze the CBC results of DS children, reporting that their hematological indices are within the expected range for children without DS. Additionally, it was found that 12.3% of them had anemia.

  8. PRELIMINARY STUDY OF HEMATOLOGICAL PARAMETERS IN HERZEGOVINIAN DONKEY

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    Dunja Rukavina

    2016-02-01

    Full Text Available Herzegovinian donkey is a very important animal resource of Bosnia and Herzegovina. There have been no works attempted at determining the normal values of hematological parameters of Herzegovinian donkey. For this reason, the objective of the present study was to investigate some hematological parameters in Herzegovinian donkey. The research was performed on 30 apparently healthy donkeys (18 female and 12 male of ages from 1 to 20 years. Blood samples (3 ml were obtained by jugular vein puncture (vena jugularis externa in vacuum tubes with EDTA. The mean value of hematocrit was 29.19 %, hemoglobin concentration 10.6 g/dl, mean corpuscular hemoglobin concentration 36.33 g/dl, white blood cells 9.33 x109/L, granulocytes (109/L 5.45 x109/L, granulocytes (% 59.47%, lymphocyte/monocyte (109/L 3.89 x109/L, lymphocyte/monocyte (% 40.53% and platelet cells 148.97 x109/L. Parameters were determined using an automated analyzer IDEXX QBC VET AutoRead. Data were analyzed by SPSS V 15. All hematological parameters (except platelet cells were consistent with the recommended reference ranges for donkeys, and the values found in literature so far. Platelet cells values were much lower than in the literature for the other donkey breeds and the recommended reference ranges for donkeys. The slight differences found between our results and those reported in the previous works confirm the need for further studies to investigate the reference values of hematological parameters of Herzegovinian donkey. This work is a contribution to the study of hematological parameters of Herzegovinian donkey, and we expect these data to be applied to the further studies.

  9. The effects of Islamic fasting on blood hematological-biochemical parameters

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    Mohamad Reza Sedaghat

    2017-06-01

    Conclusion:This study on healthy subjects suggests that fasting could affect some hematological-biochemical parameters but not all of them. Also, these changes in hematological-biochemical parameters were within the normal range and Ramadan fasting seems to be safe for healthy subjects.

  10. HIGH ALTITUDES EFFECTS ON HEMATOLOGIC BLOOD PARAMETERS

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    Hasim Rushiti

    2015-05-01

    Full Text Available The approach and the objective of this experiment are consistent with the determination of changes of blood parameters after the stay of the students at an altitude of 1800-2300 meters, for a ten-day long ski course. In this paper are included a total of 64 students of the Faculty of Sport Sciences in Prishtina, of the age group of 19-25 (the average age is 21. All students previously have undergone a medical check for TA, arterial pulse and respiratory rate. In particular, the health situation is of subjects was examined, then, all students, at the same time, gave blood for analysis. In this experiment, three main hematologic parameters were taken in consideration: such as hemoglobin, hematocrit and red blood cells. The same analyses were carried out after the 10-day stay at a high altitude. The results of the experiment have shown significant changes after the ten-day stay at high altitude, despite the previous results that show changes only after the twenty-day stay in such elevations.

  11. Some Hematologic Parameters during Different Phases of the Estrous in the Cow

    OpenAIRE

    YILDIZ, Hamit

    2014-01-01

    This study was performed on the blood samples of 7 Brown cows collected during the estrus, metestrus, diestrus and proestrus phases of the estrous cycle. We investigated whether or not there was a difference between these stages of the cycle with regard to hematologic parameters. Mean hematologic parameters determined during the period of the estrous cycles were found to be within the normal range. This study has shown that the erythrocyte count, total leukocyte count, hematocrit, hemoglobin...

  12. Hematological profile of normal pregnant women in Lagos, Nigeria

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    Akinbami AA

    2013-05-01

    Full Text Available Akinsegun A Akinbami,1 Sarah O Ajibola,2 Kabiru A Rabiu,3 Adeniyi A Adewunmi,3 Adedoyin O Dosunmu,1 Adewumi Adediran,4 Vincent O Osunkalu,4 Bodunrin I Osikomaiya,5 Kamal A Ismail,51Department of Haematology and Blood Transfusion, Lagos State University, College of Medicine, 2Department of Haematology and Blood, Lagos University Teaching Hospital, 3Department of Obstetrics and Gynaecology, Lagos State University, College of Medicine, 4Department of Haematology and Blood, Faculty of Clinical Sciences, College of Medicine, University of Lagos, 5Department of Haematology and Blood Transfusion, Lagos State University Teaching Hospital, Lagos, NigeriaBackground: Hematological profile is considered one of the factors affecting pregnancy and its outcome. Anemia is the most common hematological problem in pregnancy, followed by thrombocytopenia. Leukocytosis is almost always associated with pregnancy. The study reported here was designed to evaluate the overall mean values of seven major hematological parameters and their mean values at different trimesters of pregnancy.Subjects and methods: This examination was a cross-sectional study of 274 pregnant women who registered to attend the Lagos University Teaching Hospital or Lagos State University Teaching Hospital antenatal clinics between their first and third trimester. Blood (4.5 mL was collected from each participant into a tube containing the anticoagulant ethylenediaminetetraacetic acid (EDTA. A full blood count was performed on each sample and the results were analyzed.Results: Overall, the values obtained were (mean ± standard deviation [SD]: hematocrit level, 30.16% ± 5.55%; hemoglobin concentration, 10.94 ± 1.86 g/dL; white blood cells, 7.81 ± 2.34 × 109; platelets, 228.29 ± 65.6 × 109; cell volume 78.30 ± 5.70 fL, corpuscular hemoglobin, 28.57 ± 2.48 pg; and corpuscular hemoglobin concentration, 36.45 ± 1.10 g/dL. When grouped by trimester, the mean ± SD value of packed cell volume

  13. Hematological and Serum Biochemical Parameters of Weanling ...

    African Journals Online (AJOL)

    The objective of this study was to determine the dose-dependent effect of medroxyprogesterone acetate on hematology and serum biochemistry in weanling dogs. Nine healthy local dogs between 12 to 14 weeks of age were randomly assigned to three treatments: control, 150mg/kg and 300mg/kg of medroxyprogesterone ...

  14. Effects of Ocimum Gratissimum on the Hematological Parameters of ...

    African Journals Online (AJOL)

    The effects of crude extract of Ocimum gratissimum on some hematological parameters was studied in 32 albino Wistar rats weighing between 150 and 180 g. Increasing doses of 0.4, 0.8 and 1.2 ml of the extract were administered orally to different groups of rats for 30 days. Significant decreases were recorded in red blood ...

  15. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    AJB SERVER

    2007-01-04

    Jan 4, 2007 ... protein were measured by the methods of Allain (1974), Tinder. (1969) and Lowry et al. (1951), respectively. RESULTS AND DISCUSSION. Smokeless tobacco (Jharda) consumers and control group's erythrocyte parameters and leukocyte cell counts are listed in (Table 1). In Smokeless tobacco [Jharda].

  16. Changes in Hematological Parameters and Erythrocyte Osmotic ...

    African Journals Online (AJOL)

    The study was aimed at evaluating the changes in haematological parameters and erythrocyte osmotic fragility in lame and aged horses administered with resveratrol supplement (Equithrive joint®). A total of 16 horses of both sexes, aged 18 ± 0.65 and showing lameness grade 3 were used for the study. The horses ...

  17. Main hematological parameters of sturgeon species (Acipenseridae (review

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    M. Simon

    2017-03-01

    Full Text Available Purpose. To analyze scientific sources on the physiological, biochemical, ecological and genetic features of the main hematological paremeters and patterns of their variability in sturgeon species (Acipenseridae. To examine the fundamental aspects of lipid and protein metabolism in blood serum and the effect of biotic and abiotic factors on them. To highlight the common features of serum enzymes. Findings. A review of scientific papers revealed that although hematological parameters of sturgeons are generally similar to those of teleosts and mammals, there are a number of significant differences. In addition, many hematological parameters are characterized by species specificity, even within a family. Special attention is given to the variability of hematological parameters under the effect of factors of both internal and external environment. The paper describes the effects of sex and age, as well as the seasons of the year on the compositio of sturgeon blood. The fundamentals of the use of serum proteins in genetic and population studies are outlined. The features of the functioning of hemoglobin in sturgeon’s red blood cells are examined. The main hematological parameters involved in the formation and maturation of sexual products, and their effect on fertility are reviewed. For example, the spawners, which hadn’t put reproductive product, are characterized by a low rate of hemoglobin, increase in erythrocyte sedimentation speed and also a rise of the level of crude protein in blood and β-lipoproteid serum.The biochemical parameters (total protein and fractions, glucose, creatinine, cholesterol, the activity of some enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase in serum are examined. Practical value. The systematized data on the main hematological parameters and patterns of their variability in sturgeon species will be useful for both scientits and fish farmers. This is due to the

  18. Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Hansen, Anne-Louise S

    2011-01-01

    To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters.......To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters....

  19. Diagnostic value of hematological parameters in patients with osteoarthritis

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    Serdar Hira

    2017-03-01

    Results: There were no significant differences in WBC, RDW, PLT, RPR levels between two groups. NLR and PLR values were significantly higher in the osteoarthritis group than in the control group. RBC, MPV and PDW values were significantly lower in the osteoarthritis group than in the control group (all . MPV and RBC were negatively correlated with ESR and CRP in osteoarthritis patients. Conclusion: Hematological inflammatory markers might be useful parameters that could be used in patients with osteoarthritis. [Cukurova Med J 2017; 42(1.000: 120-125

  20. Breed-specific hematological phenotypes in the dog: a natural resource for the genetic dissection of hematological parameters in a mammalian species.

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    Jennifer Lawrence

    Full Text Available Remarkably little has been published on hematological phenotypes of the domestic dog, the most polymorphic species on the planet. Information on the signalment and complete blood cell count of all dogs with normal red and white blood cell parameters judged by existing reference intervals was extracted from a veterinary database. Normal hematological profiles were available for 6046 dogs, 5447 of which also had machine platelet concentrations within the reference interval. Seventy-five pure breeds plus a mixed breed control group were represented by 10 or more dogs. All measured parameters except mean corpuscular hemoglobin concentration (MCHC varied with age. Concentrations of white blood cells (WBCs, neutrophils, monocytes, lymphocytes, eosinophils and platelets, but not red blood cell parameters, all varied with sex. Neutering status had an impact on hemoglobin concentration, mean corpuscular hemoglobin (MCH, MCHC, and concentrations of WBCs, neutrophils, monocytes, lymphocytes and platelets. Principal component analysis of hematological data revealed 37 pure breeds with distinctive phenotypes. Furthermore, all hematological parameters except MCHC showed significant differences between specific individual breeds and the mixed breed group. Twenty-nine breeds had distinctive phenotypes when assessed in this way, of which 19 had already been identified by principal component analysis. Tentative breed-specific reference intervals were generated for breeds with a distinctive phenotype identified by comparative analysis. This study represents the first large-scale analysis of hematological phenotypes in the dog and underlines the important potential of this species in the elucidation of genetic determinants of hematological traits, triangulating phenotype, breed and genetic predisposition.

  1. Hematology

    International Nuclear Information System (INIS)

    Konrad, H.

    1976-01-01

    The latest state of nuclear medical functional diagnostics in hematology is reviewed. In addition to methods for determining the blood volume, iron kinetics, the survival time of erythrocytes as well as resorption and serum levels of vitamin B 12 kinetic investigations of thrombocytes, granulocytes, lymphocytes, and the spleen with the aid of radioisotopes are described in detail. Also included are tables with data about radiation doses to patients due to medical application of radioisotopes as well as a compilation of physical properties of radioisotopes which are used in hematological diagnosis such as 59 Fe, 51 Cr, 131 I, 125 I, 58 Co, 57 Co, 32 P, 3 H, sup(99m)Tc, 113 In. Finally, radiopharmaceuticals for hematological diagnostics are listed, which are commercially available in the German Democratic Republic. The booklet is intended for physicians working in outpatient departments and hospitals

  2. Evaluating the hematological and clinical-chemistry parameters of kratom (Mitragyna speciosa) users in Malaysia.

    Science.gov (United States)

    Singh, Darshan; Müller, Christian P; Murugaiyah, Vikneswaran; Hamid, Shahrul Bariyah Sahul; Vicknasingam, Balasingam K; Avery, Bonnie; Chear, Nelson Jeng Yeou; Mansor, Sharif Mahsufi

    2018-03-25

    Kratom (Mitragyna speciosa Korth.) from the Rubiaceae family is an indigenous tropical medicinal tree of Southeast Asia. Kratom leaves have been used for decades in Malaysia and Thailand in traditional context for its perceived vast medicinal value, and as a mild stimulant among manual labourers. Kratom consumption has been reported to cause side-effects in kratom users. To evaluate kratom's effects towards hematological and clinical-chemistry parameters among regular kratom users in Malaysia. A total of 77 subjects (n=58 regular kratom users, and n=19 healthy controls) participated in this cross-sectional study. All the surveys were conducted through face-to-face interview to elicit subject's socio-demographic characteristics and kratom use history. A full-blood test was also administered. Laboratory analysis was conducted using GC-MS to determine mitragynine content in the acquired kratom samples in order to relate mitragynine consumption with possible alterations in the blood parameters of kratom users. Findings showed that there were no significant differences in the hematological and clinical-chemistry parameters of traditional kratom users and healthy controls, except for HDL and LDL cholesterol values; these were found to be above the normal reference range for the former. Similarly, long-term kratom consumption (>5 years), and quantity of daily kratom use (≥3 ½ glasses; mitragynine content 76.3-114.8mg) did not appear to alter the hematological and biochemical parameters of kratom users. These data suggest that even long-term and heavy kratom consumption did not significantly alter the hematological and clinical-chemistry parameters of kratom users in a traditional setting. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Hematology

    International Nuclear Information System (INIS)

    Price, D.C.; Ries, C.

    1975-01-01

    This paper reviews the wide variety of radioisotopic techniques available to pediatricians in hematologic evaluation of their patients, with comments on the tracer techniques, and an indication of some new territory in splenic evaluation and nonradioactive tracers which may prove to be of considerable interest in the future. The only differences in applying these techniques to the pediatric population, compared with the adult population, lie in the different spectrum of hematologic diseases under consideration in this age group and the greater sensitivity to problems of radiation exposure which the pediatrician and the nuclear medicine physician must have in administering the isotopes in vivo. With these considerations in mind, the usefulness of such radioisotopic techniques in the evaluation of pediatric hematologic disease remains unquestionable. Radiopharmaceuticals and the radiation doses associated with the various procedures are listed. It is hoped in the future that fluorescent excitation techniques will replace at least s []me of the radioisotope techniques, obviating all considerations of patient irradiation in such instances. (auth)

  4. HEMATOLOGICAL PARAMETERS IN THE ALPINE GOATS DURING LACTATION

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    Zvonko Antunović

    2013-06-01

    Full Text Available The aim of this paper is to present haematological parameters in the Alpine goats during lactation. The study included 15 goats average age of 5 years in the 4th lactation. The blood sampling of goats was conducted on the 30th, 60th, 90th and 120th days of lactation. In the blood of goats, significant decrease in the number of red blood cells was determined, as lactation progressed, and their growth in the latter measure, but with no significant difference. Also, a significant decrease was found in the number of leukocytes as lactation progressed, whereas the 120th day of lactation showed their increase compared to the 90th day. A similar trend was found out for the content of hemoglobin and hematocrit. The mean volume of red blood cells and a significant proportion of monocytes were declining as lactation advance, while the mean concentration of hemoglobin significantly increased, and in the end of the study significantly decreased, compared to the measurement of 90th day. The above mentioned changes in hematological parameters indicate the quality of recovery and adjustment of goats in lactation.

  5. The effect of Ramadan fasting on hematological parameters

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    Jafar Nasiri

    2016-12-01

    Full Text Available Background: Ramadan fasting is an obligation for many Muslims around the world who abstain from eating and drinking for one month, which has different medical and physiological effects, such as reducing blood pressure, lipid profile, blood glucose, and body weight. It has also been hypothesized that Ramadan fasting may induce some changes in the hematologic parameters. Thus, we aimed to investigate the effect of Ramadan fasting on blood cell count (CBC, and erythrocyte sedimentation rate (ESR. Methods: In the present study, 59 adult healthy individuals, who had completed one month of Ramadan fasting were included. Fasting blood samples were analyzed for ESR, hemoglobin (Hb, hematocrit (Hct, white blood cell (WBC, platelet count (PLT, mean corpuscular Volume (MCV and mean platelet volume (MPV, one day before, on the second and last week of Ramadan and one month after Ramadan (phase I , II, III,  and IV, respectively. Results: 34 men and 25 women with an age range of 15 to 24 years participated in the study. Mean ESR increased significantly (except phase IV, in comparison phase III. Mean Hb and Hct levels were significantly greater in phase III than phase I (P

  6. Hematologic parameters in raptor species in a rehabilitation setting before release.

    Science.gov (United States)

    Black, Peter A; McRuer, David L; Horne, Leigh-Ann

    2011-09-01

    To be considered for release, raptors undergoing rehabilitation must have recovered from their initial injury in addition to being clinically healthy. For that purpose, a good understanding of reference hematologic values is important in determining release criteria for raptors in a rehabilitation setting. In this study, retrospective data were tabulated from clinically normal birds within 10 days of release from a rehabilitation facility. Hematologic values were compiled from 71 red-tailed hawks (Buteo jamaicensis), 54 Eastern screech owls (Megascops asio), 31 Cooper's hawks (Accipiter cooperii), 30 great-horned owls (Bubo virginianus), 28 barred owls (Strix varia), 16 bald eagles (Haliaeetus leucocephalus), and 12 broad-winged hawks (Buteo platypterus). Parameters collected included a white blood cell count and differential, hematocrit, and total protein concentration. Comparisons were made among species and among previously published reports of reference hematologic values in free-ranging birds or permanently captive birds. This is the first published report of reference values for Eastern screech owls, barred owls, and broad-winged hawks; and the first prerelease reference values for all species undergoing rehabilitation. These data can be used as a reference when developing release criteria for rehabilitated raptors.

  7. Correlation of hematological and biochemical parameters to severity ...

    African Journals Online (AJOL)

    Background: Acquired Immuno Deficiency Syndrome is a recognized emerging disease and rapidly established itself throughout the world. Hematological and biochemical abnormalities are common findings in patients with HIV infection. These includes anemia, thrombocytopenia, leucopenia, pancytopenia, altered liver ...

  8. Neoadjuvant chemoradiotherapy of rectal carcinoma. Baseline hematologic parameters influencing outcomes

    Energy Technology Data Exchange (ETDEWEB)

    Hodek, Miroslav; Sirak, Igor; Paluska, Petr; Kopecky, Jindrich; Petera, Jiri; Vosmik, Milan [University Hospital in Hradec Kralove, Department of Oncology and Radiotherapy, Hradec Kralove (Czech Republic); Ferko, Alexander; Oerhalmi, Julius [University Hospital in Hradec Kralove, Department of Surgery, Hradec Kralove (Czech Republic); Hovorkova, Eva; Hadzi Nikolov, Dimitar [University Hospital in Hradec Kralove, Fingerland Department of Pathology, Hradec Kralove (Czech Republic)

    2016-09-15

    The link between the blood count and a systemic inflammatory response (SIR) is indisputable and well described. Pretreatment hematological parameters may predict the overall clinical outcomes in many types of cancer. Thus, this study aims to systematically evaluate the relationship between baseline blood count levels and treatment response in rectal cancer patients treated with neoadjuvant chemoradiotherapy. From 2009-2015, 173 patients with locally advanced rectal cancer were retrospectively enrolled in the study and analyzed. The baseline blood count was recorded in all patients 1 week before chemoradiation. Tumor response was evaluated through pathologic findings. Blood count levels which included RBC (red blood cells), Hb (hemoglobin), PLT (platelet count), neutrophil count, WBC (white blood cells), NLR (neutrophil-to-lymphocyte ratio), and PLR (platelet-to-lymphocyte ratio) were analyzed in relation to tumor downstaging, pCR (pathologic complete response), OS (overall survival), and DFS (disease-free survival). Hb levels were associated with a response in logistic regression analysis: pCR (p = 0.05; OR 1.04, 95 % CI 1.00-1.07); T downstaging (p = 0.006; OR 1.03, 95 % CI 1.01-1.05); N downstaging (p = 0.09; OR 1.02, 95 % CI 1.00-1.04); T or N downstaging (p = 0.007; OR 1.04, 95 % CI 1.01-1.07); T and N downstaging (p = 0.02; OR 1.02, 95 % CI 1.00-1.04); Hb and RBC were the most significant parameters influencing OS; PLT was a negative prognostic factor for OS and DFS (p = 0.008 for OS); an NLR value of 2.8 was associated with the greatest significance for OS (p = 0.03) and primary tumor downstaging (p = 0.02). Knowledge of pretreatment hematological parameters appears to be an important prognostic factor in patients with rectal carcinoma. (orig.) [German] Die Verbindung zwischen dem Blutbild und der systemischen Entzuendungsreaktion (''systemic inflammatory response'', SIR) ist unbestreitbar und gut beschrieben. Aufgrund der

  9. Digestibility And Hematological Parameters Of Broiler Chickens Fed ...

    African Journals Online (AJOL)

    An experiment was carried out to determine the effect of replacing synthetic lysine (SL) with blood meal (BM) on apparent nutrients digestibility and hematological responses of broiler chickens. One hundred and fifty (150) unsexed Anak broiler chicks were used. There were five diets with diet (T1) containing 0.10% SL and ...

  10. Influence of Wallow on Some Hematological Parameters and Serum ...

    African Journals Online (AJOL)

    A study was conducted to determine the influence of wallow on some hematological and serum enzymes of growing pigs. Six pre-pubertal pigs of average age 2months were randomly assigned to two treatment groups, comprising of Wallow (A) and Non-Wallow facilities (B) in a Completely Randomized Design(CRD).

  11. The Effect of Alcohol Intoxications on Hematological Parameters of ...

    African Journals Online (AJOL)

    Due to the accessibility of alcohol, people around the world become readily intoxicated with it and in turn, it produces protease devastating effects in the human system. This study investigates the hematological effects of alcohol in albino rats grouped into three (A, B and C). Group A and B served as test while C served as ...

  12. Hematological Parameters in Broilers Reared in a Closed Production Farm in the Low Tropics

    Directory of Open Access Journals (Sweden)

    Berta Lucía Avilez Colón

    2015-05-01

    Full Text Available Hematology and blood chemistry are useful tools to establish a definitive diagnosis, to guide and explore the nature of pathophysiological situations that affect birds. Several diseases of poultry can change blood parameters, which are little studied in Colombia. This study aims to determine degrees of blood hematological parameters in broilers (1-42 days old reared under experimental conditions. Blood samples were taken during the second, fourth and sixth weeks of age. Average results were: hematocrit (PCV %: 27.53; hemoglobin (Hb g/dL: 9.20; mean corpuscular volume (MCV fl: 43.10; mean corpuscular hemoglobin concentration (MCHC g/dL: 33.17; erythrocytes (Eri 106 μl: 6.98; leukocytes (Leu 103μL: 19.69; absolute relative values for lymphocyte (Lin %: 72.58; heterophile (Het %: 35; monocytes (Mon %: 9.8; eosinophils (Eos %: 17.8, and basophils (Bas %: 1.08. These results are within normal ranges, and present small variations attributable to environmental conditions and to those of confinement. Therefore, further studies are needed on this topic in the region.

  13. The effects of ramadan fasting and physical activity on blood hematological-biochemical parameters.

    Science.gov (United States)

    Attarzadeh Hosseini, Seyyed Reza; Hejazi, Keyvan

    2013-07-01

    Objective(s) : Fasting during the month of Ramadan is a religious obligation and belief for healthy adult Muslims. The aim of the present study was to determine the effects of Ramadan Fasting and physical activity on 'Blood Hematological-Biochemical Parameters'. In this study, twenty-six healthy males in two experimental groups were compared in two different instances before and after the training period. The groups which were selected by convenience sampling method were divided into two non-active fasting (n=13) and active fasting (n=13) groups. For comparison purposes between groups, paired and independent sample t-test was performed, respectively, after ensuring their normality within a significance level of P≤0.05. HDL-C increased significantly in both active and non-active fasting groups, Moreover, amount of hematocrit (Hct), red blood cell count (RBC), TC, LDL, VLDL, LDL/HDL and TC/ HDL decreased significantly. Amount of hemoglobin (Hgb) and glucose reduced significantly in the active-fasting group. The variation of the means between the groups in the Hgb index and LDL/HDL were statistically significant. Fasting during the month of Ramadan by regular physical activity caused positive alterations in Hematological-Biochemical Index. These changes may be due to the alterations in diet, biology response of the body to the starving and physical activity during this month.

  14. The Importance of Hematological Parameters in Acute Respiratory Viral Infections in Children

    Directory of Open Access Journals (Sweden)

    L. A. Alekseeva

    2013-01-01

    Full Text Available Hematological studies are basic and mandatory in diagnostics and laboratory monitoring of infectious diseases, which led to their inclusion in the modern standards of laboratory examinations of children. Assessment of hematological parameters used for the provisional differential diagnosis of viral or bacterial nature of the disease. For research currently being used increasingly Hematology analyzers, which allows to facilitate and standardize the results. In this paper a comparison and differences hematological parameters practically healthy children and children with respiratory infections. Identified some changes in indicators of haemogram depending on the etiology and character of the clinical course of the disease. On the basis of the leukocyte formula defined leukocyte indices of intoxication and illustrates their importance in assessing the severity of the infection process.

  15. Effect of Simulated Intermittent Altitude on the Metabolic and Hematologic Parameters in Streptozotocin Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    mehdi Faramoushi

    2016-04-01

    Full Text Available Background & objectives: Type II diabetes is a metabolic disorder accompanied with insulin resistance of the whole body cells and is considered be the fifth cause of death in the world. Adaptation to altitude can lead to tolerance to many diseases. Therefore, the aim of this study was to determine the effect of simulated intermittent altitude on the metabolic and hematologic parameters and liver function in streptozotocin induced diabetic rats. Methods: In the current experimental study, twenty four male Wistar rats weighing 220±20 gr were randomly divided into three groups; normal control group (NC, n=8, diabetic control group (D, n=8 received fat diet for 2 weeks then were injected with streptozotocin (37 mg/kg and diabetic+hypoxia group (D+H, n=8 including diabetic rat exposed to chronic intermittent hypoxia (PiO2≈106 mm Hg, simulated altitude≈3400 m, 14% oxygen for 8 weeks. Diabetic, hematologic and lipid parameters as well as ALT and AST activities were measured in peripheral blood. Results: Our findings showed that intermittent hypoxia significantly decreased serum total cholesterol, LDL ,VLDL and triglyceride in D+H group compared to D group (p<0.05. Serum levels of fasting blood glucose and homeostatic model assessment-insulin resistance HOMA-IR( index and ALT were decreased in D+H group vs. D group p<0.05. Also, hemoglubin and hematocrite level increased in D+H group in comparison to D group p<0.05. No significant difference was detected in red blood cell count in D+H vs. D group. Conclusion: Based on resultant data, it seems that intermittent exposure to hypoxia (simulated to chronic and intermittent lodgement in altitude can be used to control of type 2 diabetes by increasing hemoglobin, decreasing insulin resistance and improving liver function as well as lipid parameters.

  16. Effects of Opium Addiction and Cigarette Smoking on Hematological Parameters.

    Science.gov (United States)

    Shahabinejad, Gholamabbas; Sirati-Sabet, Majid; Kazemi-Arababadi, Mohammad; Nabati, Saeideh; Asadikaram, Gholamreza

    2016-07-01

    The aim of the present study was to investigate the effects of opium addiction and cigarette smoking on the complete blood count (CBC). Eighty-six male subjects, including 31 opium-addicted cigarette smokers (OACS), 19 opium-addicted non-cigarette smokers (OANCS), 17 non-opium-addicted cigarette smokers (NOACS), and 19 non-opium-addicted non-cigarette smokers (NOANCS) participated in this study. The CBC test was measured in all individuals. The OACS had significantly higher white blood cell (WBC), lymphocyte, and red blood cell (RBC) count but lower in mean corpuscular volume (MCV) compared to NOANCS. The OANCS had significantly higher lymphocyte in comparison with NOACS. Our results demonstrated that the number of WBC, lymphocytes, and RBC were significantly higher, while, MCV was lower in OANCS subjects when compared to NOACS. The OACS had significantly higher level of lymphocyte in comparison with NOACS. The mean number of lymphocyte in OANCS was found significantly higher than NOACS. The smokers were shown to have significantly higher levels of WBC compared to NOANCS. Our results showed that opium-addiction, especially when associated with cigarette smoking, has intensive effects on hematological factors and these alteration might leads to greater risk for developing atherosclerosis, cardiovascular diseases, and imbalance in immune system.

  17. Relationship between body temperature, weight, and hematological parameters of black tufted-ear marmosets (Callithrix penicillata).

    Science.gov (United States)

    Pereira, Lucas Cardoso; Barros, Marilia

    2016-06-01

    Basal thermal values of captive adult black tufted-ear marmosets (Callithrix penicillata) in a thermoneutral environment were measured via different methods, along with body weight and hematological parameters. Body temperatures were recorded with rectal (RC), subcutaneous (SC) microchip transponder and infrared (left and right) tympanic membrane (TM) thermometries. Thermal values were correlated with body mass and some hematological data. Similar RC and SC temperatures were observed, these being significantly higher than the left and right TM values. SC temperature was positively correlated and in close agreement with RC measurements. Although body temperatures were not influenced by gender, capture time, or body weight, they were correlated with hematological parameters. Thus, body temperatures in this species seem to reflect some of the characteristics of the assessments' location, with SC microchip transponders being a less invasive method to assess body temperature in these small-bodied non-human primates. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. The largest reference range study for hematological parameters from Turkey: A case control study

    Directory of Open Access Journals (Sweden)

    Nilgün Tekkeşin

    2014-12-01

    Full Text Available Objectives: Accurate, reliable laboratory reference ranges are essential for effective clinical evaluation and monitoring. We present robust reference ranges established for hematology parameters using the Sysmex XT2000i analyzer. Methods: Blood samples were taken from 17409 healthy adults (19 to 49 years, 51.4% men and 48.6% women and routine hematology analysis performed. Patients were assessed as healthy on the basis of a medical history and routine medical examinations. Serum hematinic assays (vitamin B12, folate, iron and ferritin were also analyzed in order to exclude the potential anemia existence. Results: There was a statistically significant difference (p<0.001 between men and women, with the former showing higher CBC values, except WBC, neutrophil and platelet counts compared to females. Several differences were observed when compared to previously established values from Turkey, most notably in leucocytes and platelets. Our findings for CBC parameters, except leucocyte count and MCV are in general agreement with previously published data from more limited trials undertaken in other countries. Conclusions: In spite of the uncontrolled factors influencing hematological values, this study permitted to establish the new hematological reference values in Turkey, especially living near sea level. In the absence of previously detailed investigated hematological reference values in Turkey, we offered to use these results for clinical management of Turkish patients and interpretations of laboratory data for research purpose. J Clin Exp Invest 2014; 5 (4: 548-552

  19. Effect of subchronic administration of nutmeg (Myristica fragrans Houtt) ethanolic extract to hematological parameters in rat

    Science.gov (United States)

    Bachri, M. S.; Yuliani, S.; Sari, A. K.

    2017-11-01

    Nutmeg is dried kernel of broadly ovoid seed of Myristica fragrans Houtt. It has been mentioned in ethnomedical literature as aphrodisiac, stomachic, carminative, tonic, and nervous stimulant. In order to establish the safety of nutmeg, the effect of the repeated administration of nutmeg is needed. The study was aimed to determine the toxic effect of subchronic administration of nutmeg ethanolic extract to hematological parameters in rat. A total of 28 male adult Wistar rats divided into 4 groups. Group I as control was given by 0.5% CMC-suspension, group II, III, and IV were given by 50, 100, and 200 mg/kg bw, respectively, of nutmeg ethanolic extract. The treatments were administered daily for 31 days. On day 31 bloods were taken from orbital sinus. The hematological parameter consisted of the numbers of erythrocyte and leukocyte as well as hemoglobin and total protein levels were measured. The data were statistically analyzed by one way Anova followed by LSD test. All of observed hematological parameters in rats showed that there were no significant difference between the nutmeg ethanolic extract treated groups and control group. The result indicated that the subchronic administration of 50, 100, and 200 mg/kg bw of nutmeg ethanolic extract did not cause the change of hematological parameters in rat.

  20. effects of dietary xylopia aethiopica on hematological parameters ...

    African Journals Online (AJOL)

    honey

    2014-03-31

    Mar 31, 2014 ... PARAMETERS AND PLASMA LIPIDS IN MALE WISTAR RATS. *1Onyebuagu, P.C. ... rich iron content, and that its reduction in serum total cholesterol is a welcome development as an anti- .... Estimation of Prothrombin Time (PT): An aliquot (0.1ml) of platelet poor plasma (PPP) sample from each animal.

  1. Impact of nickel (Ni) on hematological parameters and behavioral ...

    African Journals Online (AJOL)

    Jane

    2011-10-17

    Oct 17, 2011 ... copper on blood and biochemical parameters of rainbow trout. (Oncorhynchus mykiss). Arch. Environ. Contamin. Toxicol. 36: 415-. 423. Dickman MD, Leung KM (1998). Mercury and organo chlorine exposure from fish consumption in Hong Kong. Chemosphere, 37: 991-1015. Eisler R, Gardener GR (1973).

  2. Correcting Effect of Therapeutic Doses of Optical Radiation on Hematological Parameters of Blood Irradiated In Vivo

    Science.gov (United States)

    Zalesskaya, G. A.; Laskina, O. V.

    2017-07-01

    We studied the effect of therapeutic doses of optical radiation on the hematological parameters of blood irradiated in vivo: hemoglobin concentration, hematocrit, and the number of erythrocytes in the peripheral blood of patients during courses of extracorporeal, overvein, and intravenous blood irradiation and after treatment. The reversible changes during the procedures were found to differ from the changes obtained after treatment completion. At the end of the treatment course, the hematological parameters had changed in different directions and became higher, the same, or lower than the initial parameters depending on the initial parameters and photoinduced changes in blood oxygenation. A compensatory effect was found for photohemotherapy on oxygen-dependent processes altering the oxygen inflow into cells as well as the generation of active oxygen species and their inhibition by antioxidant systems.

  3. Effects of long-distance running on iron metabolism and hematological parameters.

    Science.gov (United States)

    Seiler, D; Nagel, D; Franz, H; Hellstern, P; Leitzmann, C; Jung, K

    1989-10-01

    In 110 well-trained participants of a 1000-km running competition lasting for 20 days hematological parameters, iron metabolism, and their respective changes during the race were investigated. Thirty-nine men and 11 women were accustomed to wholesome vegetarian food (lacto-ovovegetarian), 52 men and 8 women consumed a conventional western diet. In each group 50% of the runners finished the race. Before the competition started red blood cell count, hematocrit, and hemoglobin were on average below the values observed in the normal population in all groups. Both male and female runners consuming the wholesome diet showed significantly lower ferritin values than those on a western diet. During the first days of the competition hemolysis occurred leading to increased serum concentrations of bilirubin and iron and decreased haptoglobin levels. Hb concentrations showed a constant decrease during the race. Serum ferritin concentration rose about twofold within the first days and then decreased again without reaching pre-race levels. Serum iron concentrations showed a significant decrease between days 3 and 6. Iron loss was caused by hematuria (25% of all urines tested), gastrointestinal blood loss (10% of all stool specimens tested), and by sweating (4.5 micrograms iron/dl sweat). Our results suggest that especially in female long-distance runners it may be difficult to supply sufficient quantities of iron with the diet.

  4. Influence of seasonality and pollution on the hematological parameters of the estuarine fish Centropomus parallelus

    Directory of Open Access Journals (Sweden)

    Robson Seriani

    2013-06-01

    Full Text Available This study investigated the hematological parameters of the tropical estuarine fish Centropomus parallelus and their use as a non-destructive biomarker for aquatic pollution. Individuals were collected, in summer and winter, at two estuaries, Cananéia (CAN and São Vicente (SVE, and blood was extracted by caudal puncture. The evaluated parameters were hematocrit (Ht, red blood cells (RBC, Mean Corpuscular Volume (MCV, and the leukocyte (WBC and thrombocyte counts. Fishes from CAN showed higher values of absolute number of thrombocytes in the summer. The fishes from SVE presented lower values of Ht and MCV in winter. Comparing the hematological parameters of fishes from these two sites, Ht, MCV, WBC and RBC were higher in fishes from SVE in the summer, whereas during the winter, Ht and thrombocytes were higher in animals from SVE. The results allow attributing the changes in the blood of fishes to seasonality and the presence of contaminants.

  5. Influence of coconut oil administration on some hematologic and metabolic parameters in pregnant rats.

    Science.gov (United States)

    Nandakumaran, Moorkath; Angelaki, Elisaveth; Al-Azemi, Nasser; Al-Sarraf, Hameed; Al-Saleh, Eyad

    2011-10-01

    Data on the effect of coconut oil intake on various hematologic and metabolic parameters in pregnant women or animals are scanty. Hence we attempted to assess the effect of oral administration of graded doses of this edible oil during pregnancy, on various hematologic and metabolic parameters in rats. Groups of pregnant Sprague Dawley rats were given oral doses of 1 ml, 2 ml, and 4 ml coconut oil twice per day, respectively. Control group of rats were given tap water. Oral feeding of oil was done continuously for a period of 20 days and at the end of the study period the animals were lightly anaesthetized with ether and sacrificed to collect blood samples for analysis. Various hematologic parameters such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hg), platelets, lymphocytes, and mean corpuscular hemoglobin concentration (MCHC) were analyzed by a hematology blood analyzer, while metabolic parameters such as cholesterol, triglycerides, urea, uric acid, creatinine, and protein were analyzed by specific analytical kits. Activities of antioxidant enzyme, superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant activity (TAO) were assessed by specific analytical kits. Statistical analysis of data was performed using a SPSS data analytical package. Oral administration of coconut oil for 20 continuous days of pregnancy did not significantly alter any of the hematologic parameters studied, compared to control group even when the oil was administered at a relatively massive dose of 4 ml/day. Administration of coconut oil appeared to decrease WBC, Hg, platelet, and lymphocyte blood concentrations in treated rats, but the difference, however, was not statistically significant (ANOVA test; p > 0.05). However, platelet concentration was significantly lower (p coconut oil compared to control group rats. Administration of coconut oil did not alter the concentrations of protein, cholesterol, urea, triglycerides, uric acid, and

  6. Lead toxicity on hematological parameters in workers with occupational exposure to lead

    International Nuclear Information System (INIS)

    Dursun, N.; Koese, K.

    1998-01-01

    The effects of lead on hematological parameters were studied in lead exposed male workers occupied for 17.84+-4.22 years in a metal powder producing factory in Kayseri, Turkey and control male workers in same city. Blood lead and plasma zinc levels were measured by Atomic Absorption Spectrophotometry (AAS) and hematological parameters by Culture Counter S. The lead exposure workers had higher lead levels (13.81+- 9.21 mug/dl) as compared to control subjects (2.37+-0.10 mug/dl). No difference was observed in the plasma zinc levels of both groups. As indices of lead exposure, red blood cell (RBC) counts, hemoglobin (Hb), and hematocrit (Hct) values significantly decreased. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC) significantly increased except MCV. There was also an increase in MCV, but it was not significant. (author)

  7. Oxamyl-induced alterations in hematological and biochemical parameters in rats

    International Nuclear Information System (INIS)

    Fayez, V.

    2003-01-01

    Effect of daily oral doses of 0.9 and 2.5 mg/kg of the carbamate insecticide oxamyl for 16 days on selected hematological and biochemical parameters in male rats was investigated. The weight of animals was significantly decreased compared to controls. The hematological studies revealed significant reduction in red blood cell count, hemoglobin and hematocrit values. Impairment of thyroid function was noticed by elevation of triiodothyronine (T 3 ) and depression of thyroxine (T 4 ). Brain acetylcholinesterase (AchE) was moderately inhibited in the first few days of exposure. However, the results of the parameters investigated indicate a moderate degree of toxicity of oxamyl following oral exposure of the doses selected

  8. Long-term effects of water pH changes on hematological parameters ...

    African Journals Online (AJOL)

    The aim of this study was to examine the effects of water pH changes on certain hematological parameters of fingerlings of common carp (Cyprinus carpio), in water with different pH (acidic and alkaline). Fingerlings of common carp were subjected to acidic (pH 5.5 and 6.5) and alkaline (pH 8.0, 8.5 and 9.0) water for 21 ...

  9. Can dosimetric parameters predict acute hematologic toxicity in rectal cancer patients treated with intensity-modulated pelvic radiotherapy?

    International Nuclear Information System (INIS)

    Wan, Juefeng; Liu, Kaitai; Li, Kaixuan; Li, Guichao; Zhang, Zhen

    2015-01-01

    To identify dosimetric parameters associated with acute hematologic toxicity (HT) in rectal cancer patients undergoing concurrent chemotherapy and intensity-modulated pelvic radiotherapy. Ninety-three rectal cancer patients receiving concurrent capecitabine and pelvic intensity-modulated radiation therapy (IMRT) were analyzed. Pelvic bone marrow (PBM) was contoured for each patient and divided into three subsites: lumbosacral spine (LSS), ilium, and lower pelvis (LP). The volume of each site receiving 5–40 Gy (V 5, V10, V15, V20, V30, and V40, respectively) as well as patient baseline clinical characteristics was calculated. The endpoint for hematologic toxicity was grade ≥ 2 (HT2+) leukopenia, neutropenia, anemia or thrombocytopenia. Logistic regression was used to analyze correlation between dosimetric parameters and grade ≥ 2 hematologic toxicity. Twenty-four in ninety-three patients experienced grade ≥ 2 hematologic toxicity. Only the dosimetric parameter V40 of lumbosacral spine was correlated with grade ≥ 2 hematologic toxicity. Increased pelvic lumbosacral spine V40 (LSS-V40) was associated with an increased grade ≥ 2 hematologic toxicity (p = 0.041). Patients with LSS-V40 ≥ 60 % had higher rates of grade ≥ 2 hematologic toxicity than did patients with lumbosacral spine V40 < 60 % (38.3 %, 18/47 vs.13 %, 6/46, p =0.005). On univariate and multivariate logistic regression analysis, lumbosacral spine V40 and gender was also the variable associated with grade ≥ 2 hematologic toxicity. Female patients were observed more likely to have grade ≥ 2 hematologic toxicity than male ones (46.9 %, 15/32 vs 14.8 %, 9/61, p =0.001). Lumbosacral spine -V40 was associated with clinically significant grade ≥ 2 hematologic toxicity. Keeping the lumbosacral spine -V40 < 60 % was associated with a 13 % risk of grade ≥ 2 hematologic toxicity in rectal cancer patients undergoing concurrent chemoradiotherapy

  10. Changes in some Hematological Parameters and Thyroid Hormones in Rats Exposed to Pulsed Electromagnetic Field

    International Nuclear Information System (INIS)

    EL-Abiad, N.M.; Marzook, E.A.; EI-Aragi, G.M.

    2007-01-01

    In the present study pulsed electromagnetic spectrum was used to evaluate the effect of exposure on some biochemical and hematological parameters in male albino rats. Three groups of rats (10 each) were exposed to 10, 15, 20 pulses of electromagnetic spectrum 3 times per week for 3 weeks, the unexposed group was considered as the control group. At the end of experiment, serum levels of thyroid hormones triiodothryronine and thyroxine (T 3 ,T 4 ) and some hematological parameters were estimated. The hematological studies revealed that exposure to electromagnetic spectrum induced significant reduction in red blood cell count(RBC),and also in hemoglobin concentration(Hb), while reticulocytic count(Ret.) was elevated in the three exposed groups, platelets count was increased only on the second exposed group, while leukocytic count (WBC's), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MGH), mean corpuscular hemoglobin concentration (MCHC) were not affected, lymphocytic count was decreased only on the second exposed group, the impairment of thyroid functions was noticed by elevation of T 3 and T 4 in the three exposed groups

  11. Effect of pulsed electromagnetic field on some biochemical and hematological parameters of female rats

    International Nuclear Information System (INIS)

    Marzook, E.A.

    2006-01-01

    The present study was designed to investigate the effect of exposure to pulsed electromagnetic spectrum on some biochemical and hematological parameters in female albino rats. A group of mature female rats was exposed to 10 pulses of electromagnetic spectrum (frequency 8-12 GHz) 3 times/week for 3 weeks. The untreated group was considered as the control group. At the end of the experiment, serum levels of malondialdehyde, thyroid triiodothyronine and thyroxine (T3, T4), α-feto protein, estradiol, calcium, urea, creatinine and other hematological parameters were estimated. The present data revealed that serum levels of estradiol, malondialdehyde, urea, creatinine, triiodothyronine and thyroxine were elevated in the exposed group while serum calcium was significantly decreased. Non-significant difference was found in the value of α-feto protein between the two groups. The hematological studies revealed that exposure of rats to electromagnetic spectrum induced significant reduction in red blood cells (RBCs), hemoglobin concentration (Hb) and in hematocrit percent (Hct%), while reticulocyte count (Ret %) was elevated in the treated group. Non-significant changes were observed in platelets, leukocyte (WBCs) and lymphocytic counts in the exposed group as compared to the control group

  12. Assessment of some biochemical and hematological parameters in treated with the organophosphorus insecticide cyanox

    International Nuclear Information System (INIS)

    ElMahdy, A.A.; Fayz, V.; Hassanin, M.M.

    2002-01-01

    The effect of single acute oral doses of 29 and 58 mg/kg of cyanox (an organophosphorus pesticide) on selected biochemical and hematological parameters in male rats were investigated. This compound inhibited blood acetylcholine esterase (AChE) significantly, over 72 hours post-treatment with the higher dose level. Serum alkaline phosphatase (ALP) was significantly depressed, 48 hr after treatment with 58 mg/kg. Serum bilirubin was elevated significantly after 48 and 24 hr of treatment with 29 and 58 mg/kg, respectively. Serum cholesterol increased significantly over 48 hr post-treatment with both dose levels.Hypertriglyceridemia was noticed only in the higher dose group. Serum total lipids were increased significantly at both dose levels. A slight treatment-related effect was observed in serum creatinine level. The hematological investigations revealed the occurrence of macrocytic normochromic anemia that was more evident in the lower dose group

  13. [The influence of probiotic fermented milk product on colon microbiota, hematological parameters and cell immunity in rats].

    Science.gov (United States)

    Kuznetsova, G G; Trushina, É N; Muatafina, O K; Cherkashin, A V; Batishcheva, S Iu; Semenikhina, V F; Sheveleva, S A

    2012-01-01

    Influence of probiotic fermented milk product on the intestinal microbiota, hematological parameters and immune status of the experiment in vivo at Wistar rats was studied. It was shown, that entering of probiotic strains of Bifidobacterium bifidum 791, Bifidobacterium longum B-379M and Lactobacillus acidophilus NK1 u Streptococcus thermophilus in composition fermented milk products in the total quantity of 2,1 x 10(7) CFU/ sm3 in digestive tract within three weeks has a positive influence on the resident of colon microbiota. Significant increasing of population levels of Bifidobacterium, Enterobacteriaceae with normal biochemical properties, registered a strong tendency to increase the content of Lactobacteria, which led to a decreasing the number of potential pathogenic transient flora with pathogenic factors. Monitoring of body mass in experimental animals has shown that including of fermented milk product with probiotic strains in diet has a positive influence on the feed uptake. Probiotic properties of the product also have stimulated effect on the immune status of the rat: improvements in cell immunity (increasing the relative amount of T-helper cells, immuneregulatory index) and hematological parameters (increase

  14. Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period

    Directory of Open Access Journals (Sweden)

    Cincović R.M.

    2012-01-01

    with hypoglycemia, hypocalcemia, hypoalbuminemia, hyperbilirubinemia, decreased hemoglobin concentration and/or red blood cell count and neutrophil to lymphocyte ratio >1 showed significantly higher concentrations of NEFA and BHB compared to cows with parameters within normal ranges. Complete ROC (receiver operating characteristics analysis showed that NEFA and BHB are important diagnostic indicators that allow the separation of cows with parameters out of the reference values from cows with normal values of parameters in the metabolic profile and blood count (0.5parameters that are out of the reference value increases with increasing concentrations of NEFA and BHB, which confirms that intense lipolysis and ketogenesis start a series of compensatory processes in the metabolism in cows. The results confirm that NEFA and BHB significantly affect the value of numerous metabolic and hematological parameters in the periparturient period. [Projekat Ministarstva nauke Republike Srbije, br. TR31062

  15. Evaluation of locally established reference intervals for hematology and biochemistry parameters in Western Kenya.

    Science.gov (United States)

    Odhiambo, Collins; Oyaro, Boaz; Odipo, Richard; Otieno, Fredrick; Alemnji, George; Williamson, John; Zeh, Clement

    2015-01-01

    Important differences have been demonstrated in laboratory parameters from healthy persons in different geographical regions and populations, mostly driven by a combination of genetic, demographic, nutritional, and environmental factors. Despite this, European and North American derived laboratory reference intervals are used in African countries for patient management, clinical trial eligibility, and toxicity determination; which can result in misclassification of healthy persons as having laboratory abnormalities. An observational prospective cohort study known as the Kisumu Incidence Cohort Study (KICoS) was conducted to estimate the incidence of HIV seroconversion and identify determinants of successful recruitment and retention in preparation for an HIV vaccine/prevention trial among young adults and adolescents in western Kenya. Laboratory values generated from the KICoS were compared to published region-specific reference intervals and the 2004 NIH DAIDS toxicity tables used for the trial. About 1106 participants were screened for the KICoS between January 2007 and June 2010. Nine hundred and fifty-three participants aged 16 to 34 years, HIV-seronegative, clinically healthy, and non-pregnant were selected for this analysis. Median and 95% reference intervals were calculated for hematological and biochemistry parameters. When compared with both published region-specific reference values and the 2004 NIH DAIDS toxicity table, it was shown that the use of locally established reference intervals would have resulted in fewer participants classified as having abnormal hematological or biochemistry values compared to US derived reference intervals from DAIDS (10% classified as abnormal by local parameters vs. >40% by US DAIDS). Blood urea nitrogen was most often out of range if US based intervals were used: 83% by US based reference intervals. Differences in reference intervals for hematological and biochemical parameters between western and African populations

  16. Relative Leukocyte Telomere Length, Hematological Parameters and Anemia - Data from the Berlin Aging Study II (BASE-II).

    Science.gov (United States)

    Meyer, Antje; Salewsky, Bastian; Buchmann, Nikolaus; Steinhagen-Thiessen, Elisabeth; Demuth, Ilja

    2016-01-01

    The length of the chromosome ends, telomeres, is widely accepted as a biomarker of aging. However, the dynamic of the relationship between telomere length and hematopoietic parameters in the normal aging process, which is of particular interest with respect to age-related anemia, is not well understood. We have analyzed the relationship between relative leukocyte telomere length (rLTL) and several hematological parameters in the older group of the Berlin Aging Study II (BASE-II) participants. This paper also compares rLTL between both BASE-II age groups (22-37 and 60-83 years). Genomic DNA was extracted from peripheral blood leukocytes of BASE-II participants and used to determine rLTL by a quantitative PCR protocol. Standard methods were used to determine blood parameters, and the WHO criteria were used to identify anemic participants. Telomere length data were available for 444 younger participants (28.4 ± 3.1 years old; 52% women) and 1,460 older participants (68.2 ± 3.7 years old; 49.4% women). rLTL was significantly shorter in BASE-II participants of the older group (p = 3.7 × 10-12) and in women (p = 4.2 × 10-31). rLTL of older men exhibited a statistically significant, positive partial correlation with mean corpuscular hemoglobin (MCH; p = 0.012) and MCH concentration (p = 0.002). While these correlations were only observed in men, the rLTL of older women was negatively correlated with the number of thrombocytes (p = 0.015) in the same type of analysis. Among all older participants, 6% met the criteria to be categorized as 'anemic'; however, there was no association between anemia and rLTL. In the present study, we have detected isolated correlations between rLTL and hematological parameters; however, in all cases, rLTL explained only a small part of the variation of the analyzed parameters. In disagreement with some other studies showing similar data, we interpret the association between rLTL and some of the hematological parameters studied here to be

  17. Phosphorus on performance, hematological, biochemical, and bone parameters of growing pigs

    Directory of Open Access Journals (Sweden)

    Jana Euclydes Drews

    2016-06-01

    Full Text Available ABSTRACT: The purpose of this study was to assess the levels of available phosphorus (AP in the diet of growing pigs selected for lean deposition on the hematological, biochemical, and bone performance parameters. A total of 64 commercial hybrid pigs (32 castrated males and 32 females; mean initial weight: 23.31±2.4kg; age: 55-90 days were used. Animals were distributed into a randomized block design with four treatments, eight replicates, and two animals (one male and one female per experimental unit. The treatments consisted of a basal diet and another three diets, which were obtained by supplementing the basal diet with dicalcium phosphate replacing the inert phosphate, resulting in four diets with 0.100, 0.180, 0.260, and 0.340% of available phosphorus. The levels of available phosphorus quadratically influenced the daily weight gain, feed consumption, and feed conversion, which increased up to the estimated levels 0.331; 0.330, and 0.302% of available phosphorus. Levels of phosphorus showed no influence on the hematological parameters, which remained within the reference values. Levels of available phosphorus increased linearly the ash and phosphorus contents in the metacarpal bone and decreased linearly the alkaline phosphatase activity. Levels of available phosphorus (0.331 and 0.302%, corresponding to a daily intake of 5.73 and 5.18g d-1 provided respectively the best results for weight gain and feed conversion in pigs with high genetic potential, without change in hematological parameters.

  18. Influence of electromagnetic radiation of industrial frequency on the peripheral blood hematological parameters of the rodents

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    Solovyev Vladimir Sergeevich

    2016-06-01

    Full Text Available The authors studied the hematological parameters of peripheral blood in outbred white mice, which were exposed to electromagnetic radiation of industrial frequency. It was shown that the long-term staying in the zone affected by this factor causes the shift in an erythron system and the change in the total number of leucocytes in animals’ blood. The effects caused by electromagnetic action are not specific and can reflect the general reaction of an organism to the extreme physical and chemical influence. Sensitivity to the studied factor depends on animal’s sex.

  19. Effect of different levels of Lepidium sativum L. on growth performance, carcass characteristics, hematology and serum biochemical parameters of broilers.

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    Shawle, Kassa; Urge, Mengistu; Animut, Getachew

    2016-01-01

    Frequent use of antibiotics to stimulate growth and health of animals leads to the development of antibiotic-resistant populations of microorganisms. In this context, alternatives like herbs and spices to antibiotics are of importance, as they are natural products. Therefore, the present experiment was designed to evaluate the performance, carcass traits, hematology and serum biochemical parameters of broilers fed ration containing varying levels of Lepidium satvium (Garden cress) seed powder as feed additive up to the age of 42 days. A total of 204 Cobb-500 day-old broiler chicks were randomly distributed into four treatments with three replicates of 17 chicks each. Garden cress (GC) was included in the ration at 0 (0GC), 0.75 (0.75GC), 1.50 (1.5GC) and 2.25 % (2.25GC) in each treatments. Growth performance, carcass characteristics, heamatology, serum biochemical and economic efficiency parameters were observed. The crude protein, ether extract and crude fiber content of GC were 22.4, 25.7 and 10.5 %, respectively. Daily dry matter intake and average daily gain during the entire experimental period were affected (p  0.05). Sex differences were significant for eviscerated, carcass and kidney percentages with greater values for females than males. All hematological parameters were within the normal range. However, inclusion of GC improved (p health of broilers. Based on the production parameters used in the study, GC can be included as feed additive at a level of 0.75 % in the total ration for better and positive results on biological performance and health status of broilers.

  20. Effect of taking dietary supplement on hematological and biochemical parameters in male bodybuilders an equation model

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    Meamar, Rokhsareh; Maracy, Mohammad; Nematollahi, Shahrzad; Yeroshalmi, Shemouil; Zamani-Moghaddam, Ali; Ghazvini, Mohammad Reza Aghaye

    2015-01-01

    Background: The improved physical action following administration of supplements to bodybuilders was supported by changes in laboratory parameters. Despite the fact that these supplements are sometimes associated both advantage and side effects, this study were conducted for the purpose of evaluating the possible effects of some commonly used supplements in bodybuilders on the hematological and biochemical parameters. Materials and Methods: In this study, we included 40 male bodybuilders as cases and 40 controls in the age group of 20-40 years. They used different kinds of supplements for 1 year. In general, all the supplements used were classified into two groups: hormonal and non-hormonal. Laboratory tests were requested for evaluation of hematological and biochemical parameters. Results: In an equation model, we found that weight (P = 0.024), duration of bodybuilding (P bodybuilders. The available supplements are unchecked and not approved by the US Food and Drug Administration (FDA). More studies should be designed for a better and precise administration of each supplement in athletes. PMID:26793253

  1. Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman "A preliminary study"

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    Shahab Al-Bulushi

    2017-04-01

    Full Text Available Aim: In Sultanate of Oman, goats are considered as one of the most important livestock in which there are many breeds of goat such as Batinah, Jabali, Dhofari, Jabal Al-Akhdar, Sahrawi, and Sahrawi Musandam. Little hematological and biochemical information is known on Omani goat breeds; therefore, the main purpose of this study was to determine reference baseline data regarding hematological and biochemical values of different Omani goat breeds. Materials and Methods: A total of 30 healthy animals of different Omani goat breeds (Jabali, Jabal Al-Akhdar, Sahrawi, and Sahrawi Musandam were selected randomly from different areas in Sultanate of Oman. The blood samples were collected from the jugular vein into two tubes for blood hematology and biochemical analysis. Statistical analysis was applied by using GraphPad Prism 7 software to calculate the minimum and maximum values to determine the range, mean, standard deviation of the mean and the p value. Results: No statistically significant variation in most hematological and biochemical parameters was found among the Omani goat breeds. The results of blood hematology revealed that the mean white blood cells (14.6±3.32 x103/μL, and the percentage of neutrophils in Omani goats (60.87±8.46% were higher than that in most goat breeds. Higher values of red blood cells (12.8±1.28 x106/μL, hemoglobin (10.4±1.92 g/dl, hematocrit (38.29±4.06%, and lower values of mean corpuscular HGB concentration (27.05±3.5 g/dl were observed in Omani goat breeds comparing to that in the other goat breeds. Lower values of total bilirubin (0.22±0.03 mg/dl, blood urea nitrogen (14.62±2.66 mg/dl, and cholesterol (48.58±19.05 mg/dl were found in Omani goat breeds when compared to that of the other goat breeds. Conclusion: The obtained results are considered as the first values to be published for the different Omani goat breeds. This study is considered as preliminary study which can be used as a reference for further

  2. Safety Profile of Meswak Root Extract on Liver, Kidney, Sexual Hormones and Hematological Parameters of Rats

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    Abeer Y. IBRAHIM

    2012-02-01

    Full Text Available This study was conducted to investigate the safety profile of Salvadora persica (Salvadoraceae aqueous alcoholic root extract by carrying out acute and sub-chronic toxicity assessment in order to find out any side effect of the traditionally using of these root sticks. Regarding to acute toxicity test, mice were administered the extract up to 5 g kg-1, intraperitoneally. Animals were then observed for behavioural changes; signs of toxicity, and mortality within 24 h. Surviving mice were monitored for 7 days for signs of delayed toxicity. In the sub-chronic toxicity test, rats were daily treated with the extract at a dose of 400 mg kg-1 intraperitoneally, for 30 days. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. In the acute toxicity test, the extract was practically non-toxic showing no mortality and visible signs of delayed toxicity. The LD50, given intraperitoneally, was estimated to be 4 g kg-1. Administration of extract (at a dose of 400 mg Kg-1 b.wt. to male and female rats for 30 days did not produce any significant (P < 0.05 effect on hematological and most biochemical parameters also vital organs weights. The root extract showed adverse effects on sexual hormones, by increasing estrogen secretion and reducing testosterone level in male rats. At the same time, the extract reduces progesterone level in female satellite group. Overall, Meswak aqueous extract is safe concerning liver and kidney functions and hematological assessments; however, it induces reversal effect on sexual hormones levels determined in sera.

  3. Hematologic, Biochemical, and Endocrine Parameters in Horses With Tooth Resorption and Hypercementosis.

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    Earley, Edward T; Rawlinson, Jennifer R; Baratt, Robert M; Galloway, Stephen S; Smedley, Rebecca C; Scarlett, Janet M; Refsal, Kent R; Dotzel, Allison R; Cox, Victor S; Perkins, Gillian A

    2017-09-01

    Equine odontoclastic tooth resorption and hypercementosis (EOTRH) is a frequently diagnosed condition in adult horses. The underlying etiology is still unknown. Hematologic, biochemical, and endocrine values have not been reported in EOTRH-affected horses. The main objective of the study was to describe the hematologic, biochemical, and endocrine parameters in horses with EOTRH. Descriptive cross-sectional study of client-owned animals with EOTRH. A complete blood count, biochemistry panel, and endocrine profile were performed in horses diagnosed with EOTRH. Diagnosis was based on oral and radiographic examination findings and confirmed with histopathology. Eighteen horses with EOTRH aged 10 to 32 years from various regions of the United States were sampled. The only consistent abnormality on the complete blood cell count and chemistry panel was hypoalbuminemia (88%). Endocrine parameters demonstrated no major abnormalities in the functioning of the thyroid and pituitary pars intermedia. The parathyroid hormone concentration was increased in 7 (47%) of 15 horses with an elevated 25-hydroxy vitamin D in 3 (17%) of 17 horses. Main Limitations: The main limitations of this study are the small sample size and lack of age-matched and management-matched control horses. The relevance of elevated parathyroid hormone in this study cannot be determined due to the lack of age-based controls and large population studies. With the small population evaluated in this study, there are no obvious hematological, biochemical, and endocrine changes evident. Further evaluation with signalment-matched controls will be necessary to evaluate some trends noted in the laboratory values.

  4. The effects of low dose aluminum on hemorheological and hematological parameters in rats

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    Turgut, Sebahat; Bor-Kucukatay, Melek; Emmungil, Guelten; Atsak, Piray; Turgut, Guenfer [Pamukkale University, Department of Physiology, Medical Faculty, Denizli (Turkey)

    2007-01-15

    Aluminum (Al) is a nonessential element and humans are constantly exposed to Al as a result of an increase in industrialization and improving technology practices. Al toxicity can induce several clinical disorders such as neurotoxicity, gastrointestinal toxicity, hepatotoxicity, bone diseases, and anemia. This study aimed at evaluating the possible effects of short term and low dose Al exposure on hemorheological and hematological parameters in rats. Fourteen young, male Wistar albino rats were divided into two groups: 1 mg/200 g body weight of aluminum sulfate Al{sub 2}(SO{sub 4}){sub 3} was injected intraperitoneally to the first group for two weeks, three times a week. The animals of the control group received only physiological saline solution during this period. At the end of the experimental period, anticoagulated blood samples were collected and hematological parameters were determined using an electronic hematology analyzer. Red blood cell (RBC) deformability and aggregation were measured using an ektacytometer (LORCA) and plasma and whole blood viscosities were determined with a Wells-Brookfield cone-plate rotational viscometer. Significant decreases in mean corpuscular volume (MCV), red blood cell (RBC) deformability at low shear stress levels, the aggregation half time (t1/2) and the amplitude (AMP) of aggregation and significant increments in whole blood viscosity (WBV) at native and 40% hematocrit (Hct) of Al-treated rats have been observed. In conclusion, low dose Al{sub 2}(SO{sub 4}){sub 3} exposure for a short-time may be responsible for alterations in either rheological properties of blood or hemorheological properties through a remarkable effect on RBC membrane mechanical properties.These alterations may also play an important role in the development of anemia in the Al-treated animals. (orig.)

  5. Biochemical, hematological and histological parameters induced by cypermetherin toxicity in domestic rabbits.

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    Dahamna, S; Harzallah, D; Boussahel, S; Belgeit, A; Merghem, M; Bouriche, H

    2010-01-01

    Cypermetherin is widely used in Algeria; this insecticide belongs to the group of pyrethroids classified by the World Health Organization as moderately harmful class II (WHO, 2005). This study was conducted to search the effect of non lethal dose on biochemical parameters, hematological and histological parts of the organs. Male of domestic rabbits Oryctolagus cuniculus (1 kg) received per week and per gavage 1/10 LD50 of Cypermetherin (ARRIVO 25%, active substance of Cypermetherin 250 g/l). blood was collected 72 hours after the treatment. Enzyme activities were assayed in the plasma samples for Glutamate oxalo acetate transaminase (GOT), Glutamate pyruvate transaminase (GPT), Alcaline phosphatase (AlcP), creatinine (Crea) and glucose. Red blood (RBC) cells and white blood cells (WBC) were calculated too. The results indicated a significant increase in transaminases GOT and GPT, and AlcP explain a high energy generating product and dysfunction of the liver. A decline in Crea, Hb, RBC and WBC which is related to the immunity, this is probably due to cell lyses explain the effect of Cypermetherin on erythropoeisis. Histological examination confirmed the biochemical tests by the observation of inflammatory infiltrate and perilobular fibrosis. In conclusion, Cypermetherin with used dose affects biochemical, hematological and histological parameters of the rabbits.

  6. Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

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    Frelich Angela

    2008-09-01

    Full Text Available Abstract D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.

  7. Use of hematological parameters in evaluation of treatment efficacy in cutaneous leishmaniasis

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    Bilal Sula

    2015-12-01

    Full Text Available Objective: In the present study we investigated the role of hematological parameters, including neutrophil/lymphocyte ratio and platelet/lymphocyte ratio, mean platelet volume and platelet distribution width in the evaluation of treatment efficacy in adult patients diagnosed with cutaneous leishmaniasis. Methods: The study group included 45 adult patients diagnosed with cutaneous leishmaniasis and treated as inpatients in the dermatology clinic between 2011 and 2014. A group of 45 healthy adults served as a control group. Results: Pre- and post-treatment white blood cell count, neutrophils, and lymphocytes were significantly reduced among the patient group relative to the control group. Platelet distribution width, red cell distribution width, neutrophil/lymphocyte ratio and platelet/lymphocyte ratio were significantly elevated among the patients compared to the healthy subjects. Pre-treatment white blood cell, lymphocyte and platelet counts were significantly elevated compared to post-treatment counts among the patient cohort. Treatment was associated with reduced eosinophil count, neutrophil/lymphocyte ratio and platelet/lymphocyte ratio relative to pre-treatment status. Conclusion: Routine hematological testing results such as platelet/lymphocyte ratio, white blood cell count, neutrophil count, red cell distribution width, platelet distribution width, and mean platelet volume may be clinically significant markers of the inflammatory state useful in the evaluation of early treatment efficacy among patients with cutaneous leishmaniasis. J Microbiol Infect Dis 2015;5(4: 167-172

  8. Can Hematologic Parameters be an Indicator of Metabolic Disorders Accompanying Rosacea?

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    Akin Belli, Asli; Kara, Asude; Ozbas Gok, Seyran

    2017-07-01

    Recently, diverse hematologic parameters have been used as an indicator of the presence or severity of inflammatory and cardiovascular diseases. Our aim was to investigate the ratios of neutrophils to lymphocytes (NL), monocytes to high-density lipoprotein (HDL) cholesterol (MHC), and platelets to lymphocytes (PL) in patients with rosacea in comparison with the control group and determine whether there was a correlation between these ratios and metabolic disorders in patients with rosacea. We conducted a case-control study on 61 patients with rosacea and 60 healthy controls between January 2015 and January 2016 at the Dermatology Outpatient Clinic, Mugla, Turkey. Demographic data, biochemical parameters, hematologic parameters and ratios, the presence of metabolic syndrome (MS), and the presence of insulin resistance (IR) in the participants were recorded. Sixty one patients with rosacea (16 men, 45 women) and 60 controls (13 men, 47 women) were included in the study. The NL ratio, mean levels of low-density lipoprotein (LDL) and total cholesterol, triglyceride, C-reactive protein (CRP), systolic and diastolic blood pressures, and the presence of IR were significantly higher in patients with rosacea than in controls. In the rosacea group, the MHC ratio was significantly higher in patients with rosacea with IR and MS. Moreover, only the MHC ratio was an independent predictor of MS according to univariate logistic regression analysis. The cutoff value of MHC on admission for predicting MS in patients with rosacea was 0.013.The higher levels of NL ratio and IR in the rosacea group corroborate the previous studies demonstrating a high level of cardiovascular risk factors in patients with rosacea. The MHC ratio may be used as a simple and inexpensive method to predict metabolic disorders in patients with rosacea.

  9. Reference intervals for hematological parameters of animals bred and kept at the vivarium of the Faculty of Medicine of the State University of São Paulo

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    Marilda Osti Spinelli

    2014-03-01

    Full Text Available Knowledge of specific hematological parameters of laboratory animals used in experiments is highly relevant to evaluate functional alterations of their vital organs. Hematological reference rates for rats, mice, rabbits and hamsters, bred in conventional healthy conditions and derived from the Vivarium of the FMUSP’s Vivarium Center are established. One hundred and twenty mice of the strains BALB/c, C57BL / 6 and Swiss, 80 Wistar rats, 80 Golden hamsters and 80 New Zealand rabbits, males and females, apparently normal and healthy, over 10-weeks-old, were employed in current experiment. The analysis was determined by flow cytometry process (CELM CC510 involving the separation of erythrocytes and leukocytes, and cell identification by the suspension method and quantification. Hematological derivatives such as hematometric indexes (application of equations and morphological quantification of leukocytes and erythrocytes were determined by scanning/differential (Panótico-commercial, whereas hemoglobin dosage was determined by spectrophotometry (CELM E210D. Results are given as means and standard deviants of hematological profiles in the blood of the animals grouped by species, strain and sex. Data are an important asset for researchers in their analysis of experimental results. CEAU Certificate n.° 251/11 HCFMUSP.

  10. Comparison of hematological parameters in untreated and treated subclinical hypothyroidism and primary hypothyroidism patients

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    Haamid Bashir

    2012-11-01

    Full Text Available  Abstract Backgrounds: Thyroid hormones play an important physiological role in human metabolism. Erythrocyte abnormalities are frequently associated with thyroid disorder. However, they are rarely investigated and related to the subclinical and primary hypothyroidism in Kashmiri Patients. In this study an attempt was made to study hematological parameters in untreated and treated subclinical hypothyroidism and primary hypothyroidism patients. Methods: This retrospective study included 600 subjects, among which were untreated subclinical hypothyroid (n=110, treated subclinical hypothyroid (n=110, untreated primary hypothyroid (n=100, treated primary hypothyroid (n=100 and euthyroid (n=180. This study was carried out at Department of Biochemistry, Government Medical College Srinagar. The hematological parameters and thyroid profile of the subjects were assessed by the Sysmex (Italy and ECLIA (Germany 2010 automatic analyzer. Mean, standard deviation (SD, analysis of variance (Two-way ANOVA, and multiple comparisons were used to report our results, with p<0.05 or p<0.01 considered as statistically significant. Results: In this study group we compared the hematological parameters in these groups, untreated subclinical hypothyroid, treated subclinical hypothyroid, untreated primary hypothyroid, treated primary hypothyroid and euthyroid. We found that hematological parameters like Hb, RBC, MCV, HCT, RDW,RBC% were significantly increased in untreated subclinical hypothyroidism and untreated primary hypothyroidsm, with the p value being less than 0.05 whereas, in treated SCH & Pr. Hypothyroid, results were insignificant. The results reported in these groups as mean±SD, were statistically tested by ANOVA and multiple comparison tests. In untreated subclinical hypothyroid the values were: Hb (10.83±1.33 g/dl , RBC (4.21±0.66 106/μl, MCV (84.56±6.84 fL, HCT (38.5±2.2 %, RDW (17.91±2.37 fL, RBC% (84.36±13.2 % and in untreated primary

  11. Dietary effects of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss).

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    Yeganeh, Sakineh; Teimouri, Mahdi; Amirkolaie, Abdolsamad Keramat

    2015-08-01

    This study evaluated the effects of diets containing 0, 2.5, 5, 7.5 and 10% of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Fish (n=180; 101±8 g) were randomly divided into fifteen 300 L fiberglass tanks in triplicates for a period of ten weeks. The RBC, WBC, hemoglobin, total protein and albumin levels increased significantly in the groups supplemented with S. platensis. Dietary inclusion of S. platensis had no significant effects on hematocrit, cholesterol, triglyceride and lactate of the blood. HDL-cholesterol was larger in rainbow trout fed 10% S. platensis in comparison with the other diets, whereas LDL-cholesterol significantly decreased with increasing of S. platensis inclusion. Cortisol and glucose significantly decreased with increasing of S. platensis inclusion. The present results demonstrate that inclusion of 10% S. platensis can be introduced as an immunostimulant in rainbow trout diets. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Determination of the influence of fluid therapy on hematological and urinary parameters in dogs

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    Mariah Gois Ceregatti

    2016-09-01

    Full Text Available ABSTRACT. Ceregatti M.G., Volpato J., Mattoso C.R.S., da Costa A., Weinert N.C., Tocheto R., Comasseto F. & Saito M.E. [Determination of the influence of fluid therapy on hematological and urinary parameters in dogs.] Determinação da influência da fluidoterapia nos parâmetros hematológicos e urinários em cães. Revista Brasileira de Medicina Veterinária, 38(3:292-298, 2016. Departamento de Medicina Veterinária, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, Lages, SC 88520-000, Brasil. E-mail: jully_volps@hotmail.com The aim of the present study was to evaluate the effects of different fluid infusion rates on hematologic and urinary parameters in dogs. A total of 30 mixed-breed, male dogs were distributed into three groups. Fluid (0.9% NaCl was administered during a one-hour period at rates: G5 (5 mL/ kg; G10 (10 mL/ kg; G50 (50 mL/kg. Blood samples for erythrogram, and urine to perform urine specific gravity were taken over the following time points studied. Urinary volume produced was also evaluated during the same time points. Erythrogram values were statistically lower between T10 and T240 in G10 and G50, compared to T0, and the lowest values were found in G50. Urine specific gravity decreased statistically between all time points only in G50. Statistical differences were found in urinary output between groups, with higher volumes in G50 in most of the time points studied, it can be concluded that fluid therapy changes hematologic indices in rates of 10 and 50 mL/ kg for one hour. At least a four hour period must be respected after the end of infusion to collect blood for erythrogram samples. Fluid therapy affects the urine specific gravity only at high infusion rates (50 mL/kg for 1 hour, and in this case urine samples should be collected after more than 4 hours after the end of infusion, although lower rates (5 and 10 mL / kg for 1 hour do not interfere with urine specific gravity.

  13. Hematological parameters and prevalence of anemia among free-living elderly in south Brazil

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    Vanessa Sgnaolin

    2013-01-01

    Full Text Available OBJECTIVE: The aims of this study were to analyze the hematological parameters, the prevalence of anemia and the association between anemia and socioeconomic conditions in an elderly community-based population. METHODS: A population-based study was performed as part of the Multidimensional Study of the Elderly in Porto Alegre, Brazil (EMIPOA. An initial total of 1058 community residents aged 60 years and older were interviewed. Of these, 392 agreed to have a physical evaluation and a blood sample was taken from each. The hematological parameters analyzed in the blood samples included the hemoglobin concentration, mean cell volume (MCV, mean corpuscular hemoglobin concentration (MCHC and red cell distribution width (RDW. The association between the variables and the diagnosis of anemia was assessed using the chi-squared test and a multiple logistic regression model. RESULTS: The overall prevalence of anemia was 12.8%. Anemia was present in 13.7% of women and in 10.4% of men. Normocytic normochromic anemia without anisocytosis was the most common type of anemia (46%. The assessment of erythrocyte morphology showed significant differences between anemic and non-anemic individuals (microcytosis = 12% vs. 1.5%, hypochromia = 40% vs. 8.8%, and anisocytosis = 26% vs. 7%. In the analysis of socioeconomic conditions, significant differences were found in respect to age and race. CONCLUSION: The prevalence of anemia increases with age and is associated with race, microcytosis, hypochromia and anisocytosis. Anemia is not a condition that should be associated only with the aging process, as it may be due to pathological conditions that occur most frequently in this age group. As a result, a diagnosis of anemia warrants adequate clinical attention.

  14. Comparative effect of clopidogrel plus aspirin and aspirin monotherapy on hematological parameters using propensity score matching

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    Hayasaka M

    2013-02-01

    Full Text Available Masatoshi Hayasaka,1 Yasuo Takahashi,2 Yayoi Nishida,2 Yoshikazu Yoshida,1 Shinji Hidaka,3 Satoshi Asai41Department of Pharmacy, Nihon University Itabashi Hospital, Tokyo, 2Division of Genomic Epidemiology and Clinical Trials, Clinical Trials Research Center, Nihon University School of Medicine, Tokyo, 3Laboratory of Pharmaceutical Regulatory Science, Department of Pharmacy, School of Pharmacy, Nihon University, Chiba, 4Division of Pharmacology, Department of Biomedical Sciences, Nihon University School of Medicine, Tokyo, JapanBackground: Clopidogrel and aspirin are antiplatelet agents that are recommended to reduce the risk of recurrent stroke and other cardiovascular events. Dual antiplatelet therapy with clopidogrel and aspirin has been shown to increase the risk of hemorrhage, but the effects of the drugs on laboratory parameters have not been well studied in real-world clinical settings. Therefore, we evaluated and compared the effects of combination therapy with clopidogrel plus aspirin and aspirin monotherapy on laboratory parameters.Methods: We used data from the Nihon University School of Medicine Clinical Data Warehouse obtained between November 2004 and May 2011 to identify cohorts of new users (n = 130 of clopidogrel (75 mg/day plus aspirin (100 mg/day and a propensity score matched sample of new users (n = 130 of aspirin alone (100 mg/day. We used a multivariate regression model to compare serum levels of creatinine, aspartate aminotransferase, and alanine aminotransferase, as well as hematological parameters including hemoglobin level, hematocrit, and white blood cell, red blood cell, and platelet counts up to 2 months after the start of administration of the study drugs.Results: There were no significant differences for any characteristics and baseline laboratory parameters between users of clopidogrel plus aspirin and users of aspirin alone. Reductions in white blood cell and red blood cell counts, hemoglobin levels, and

  15. Validity of hematologic parameters in identification of early and late onset neonatal infection.

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    Varsha; Rusia, Usha; Sikka, Meera; Faridi, M M A; Madan, Nishi

    2003-10-01

    This study was designed to evaluate the utility of hematological parameters and C-reactive protein (CRP) to formulate a sepsis screen to detect sepsis in early and late onset infection. Hundred and fifty neonates clinically suspected of bacterial infection, based on risk factors and/or clinical features were selected for the study. Blood was collected by venipuncture at the time of admission in all neonates. A total leukocyte count (TLC), differential leukocyte count (DLC), its derivatives [Total neutrophil count (TNC or T), ratio of immature to total neutrophil count (I/T), ratio of immature to mature neutrophil count (I/M)] and CRP were obtained. TLC = 10x10(9)/L, TNC = 8x10(9)/L, I/T = 0.16, I/M = 0.25 and CRP = 0.6 mg/dl were found to be good parameters in detection of sepsis. During the first three days of life leukopenia, neutropenia, elevated I/T ratio, elevated I/M ratio and CRP were good diagnostic aids while after 3 days of life CRP was the best single test. This emphasizes use of multiple indicators for detection of sepsis. Using these parameters a sepsis screen was formulated which detected >90% of proven early and late onset sepsis suggesting that other neonates with positive sepsis screen but blood culture negativity may have been truly infected.

  16. The effects of Ramadan fasting and physical activity on body composition and hematological biochemical parameters

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    Seyyed Reza Attarzadeh Hosseini

    2014-09-01

    Full Text Available Introduction: Hunger and reduction in regular energy intake can lead to a number of problems based on their intensity. For instance, low energy level can cause blood cell production to decline or it may pose a higher risk of anemia. It can also weaken the immune system and platelet aggregation or negatively affect clot formation. This study aimed to have a closer look at fasting and regular physical activity and their impacts on body composition and blood hematological-biochemical parameters among professional wrestlers. Method: In this semi-experimental study, 9 subjects were selected by convenience sampling. The selected training program included participation in this exerciseprogram, 90 min per session, 6 times per week for a period of one month. Blood samples were obtained four times: before the start of Ramadan, 2 weeks after the start, during the last week and 2 weeks after the end of Ramadan. To make intra-group comparison, repeated measure analysis of variance was used. For all statistical comparisons, the level of significance was considered at PResults: Body weight and red blood cell count (RBC dropped significantly at the end of Ramadan (Respectively P= 0.001 and P=0.034. However, the number of white blood cells (WBC and circulating platelets (PLT significantly increased during fasting (Respectively P= 0.048 and P=0.042. As a matter of fact, PLT and WBC were the only factors which dramatically increased during fasting. Intra-group variations of tetracycline (TC, low-density lipoprotein (LDL, LDL: high-density lipoprotein (HDL, triglyceride (TG: HDL and TC: HDL reduced at the end of Ramadan. However, HDL levels ​​drastically increased during fasting (P≤0.05. Conclusion: Based on the results of the research, despite being a regular activity and fasting has beneficial effects on lipid profile in athletes, however, they can with tangible changes in hematological factors may lead to weaken the immune system of athletes.

  17. The effect of acetylsalicylic acid and meloxicamon hematological parameters in rats

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    Ćupić Vitomir

    2015-01-01

    Full Text Available In this work there was investigated the effect of two nonsteroidal antiinflammatory drugs (NSAIDs, acetylsalicylic acid or aspirin (nonselective cyclooxygenase inhibitor - COX1 i COX2 and meloxicam (selective cyclooxygenase inhibitor - COX2 on certain hematological parameters in rats. The objective of the work was to determine whether (and to which extent, these drugs, after multiple peroral application, influence erythrocyte number, concentration of hemoglobin, hematological indices (mean corpuscular value - MCV; mean concentration of hemoglobin in erythrocytes - MCH; mean corpuscular hemoglobin concentration - MCHC, hematocrit, number of platelets, leukocytes, neutrophilic leukocytes, lymphocytes and monocytes. The experiment was conducted in in vivo conditions on 70 clinically healthy Wistar strain male rats, 10 to 12 weeks of age and body weight 250 to 300 g. The rats were divided into seven groups and they were daily perorally (by probe given aspirin (ASCOPIR at doses of 30, 40 and 80 mg/kg b.m. (I, II and III groups, or meloxicam (METACAM at doses of 100, 125 and 250 μg/kg b.m. (IV, V and VI groups, for seven days. The seventh group was a control one and they were given only saline. The obtained results showed that: acetylsalicylic acid in maximum dose tested (80 mg/kg b.m. statistically significantly reduced the number of platelets (p<0,05, the number of leukocytes (p<0,05, the number of lymphocytes (p<0,05 and the number of monocytes (p<0,05, while on the other side, meloxicam in maximum dose tested (250 μg/kg, statistically significantly reduced the mean corpuscular value (MCV, and increased the number of platelets (p<0,05, relative to the control value.

  18. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase.

    Science.gov (United States)

    Rodríguez-Dennen, Fernando; Martínez-Ocaña, Joel; Kawa-Karasik, Simón; Villanueva-Egan, Luis; Reyes-Paredes, Norberto; Flisser, Ana; Olivo-Díaz, Angélica

    2011-05-06

    Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp) 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP) and the active labor phase (ActLP). Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  19. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase

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    Flisser Ana

    2011-05-01

    Full Text Available Abstract Background Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP and the active labor phase (ActLP. Methods Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Results Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Conclusion Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  20. Effects of Low-Intensity Continuous Ultrasound on Hematological Parameters of Rats

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    Mehrpour M.

    2016-09-01

    Full Text Available Background: Low intensity ultrasound (US has some well-known bio-effects which are of great importance to be considered. Objective: We conducted the present study to investigate the effects of low intensity continuous ultrasound on blood cells count in rat. Methods: Rats were anesthetized and blood samples were collected before US exposure. Then, they were exposed to US with nominal intensity of 0.2 W/cm2 at frequency of 3 MHz for a period of 10 minutes and this protocol was repeated for 7 days. Twenty four hours after the last US exposure, secondary blood samples were collected and the changes in blood parameters were evaluated. Results: Analysis revealed that platelets, hematocrit (HCT and hemoglobin (HGB were significantly different between experimental and sham groups but no difference between sham and control groups was observed. The results show that HCT and HGB of exposed rats were significantly reduced. Conclusion: This study shows that low intensity US may lead to side effects for hematological parameters such as reduction in the levels of HGB and HCT.

  1. THE IMPACT OF TOXIC HEAVY METALS ON THE HEMATOLOGICAL PARAMETERS IN COMMON CARP (CYPRINUS CARPIO L.

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    R. Vinodhini ، M. Narayanan

    2009-01-01

    Full Text Available The aim of the present investigation was to determine the effect of heavy metal pollutants such as cadmium, chromium, nickel and lead in aquatic system on common carp (Cyprinus carpio L. by using a set of biochemical parameters. The experimental group of fish was exposed to a sublethal concentration of 5 mg/L of combined (Cd+Pb+Cr+Ni metal solution containing 1.25 mg/L of each metal ion (1/10th of LC 50/48 h for a period of 32 days. The results indicated that the values of the hemoglobin were in the range of 55.30±1.20 g/L to 74.55±1.33 g/L (p<0.001 and the packed cell volume was in the range of 26.72±0.26% to 30.68±0.43% (p<0.01. Concentrations of red blood cells, blood glucose and total cholesterol were significantly elevated. The level of serum iron and copper was increased. The results showed the decreased activity of vitamin C during chronic exposure to toxic heavy metals, which indicates the presence of reactive oxygen species–induced peroxidation. The study suggested that the presence of toxic heavy metals in aquatic environment has strong influence on the hematological parameters in the fresh water fish common carp (Cyprinus carpio L..

  2. LOW-FREQUENCY LOW INTENSITY MAGNETIC FIELD (50 Hz; 2,7 mT INFLUENCES ON HEMATOLOGICAL PARAMETERS FOLLOWING CHEMICAL SYMPATHECTOMY IN WISTAR RATS

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    Calin Maniu

    2007-08-01

    hydroxydopamine infusion with 25 mg/kg i.p. desipramine (Sigma to protect noradrenergic projections. 12 days after the operation, the hematological parameters (the total number of erythrocytes, hematocrit and hemoglobin level were evaluated. The sympathectomy-induced severe reduction in hematological parameters under low-frequency low intensity magnetic field exposure.

  3. Comparison of hematologic and serologic profiles of broiler birds with normal and severe degrees of white striping in breast fillets.

    Science.gov (United States)

    Kuttappan, V A; Huff, G R; Huff, W E; Hargis, B M; Apple, J K; Coon, C; Owens, C M

    2013-02-01

    White striping is the white striation occasionally observed parallel to the direction of muscle fibers in broiler breast fillets and thighs at the processing plant. Broiler breast fillets can be categorized as normal (NORM), moderate (MOD), or severe (SEV) based on the degree of white striping. Histologically, SEV fillets are characterized by the highest degree of degeneration of muscle fibers along with fibrosis and lipidosis when compared with NORM. The present study was undertaken to compare the hematologic and serologic profiles of broilers with NORM and SEV degrees of white striping to get more information on the systemic changes associated with the condition. Day-old male broiler chicks of a commercial strain were grown on the same diet in 6 replicate pens (n = 32 birds/pen). Blood samples (5 mL) were collected from the wing vein of each bird on the day before processing for analyzing hematologic and serologic profiles. At 63 d, the birds were weighed and processed in a commercial inline processing system. Weight of the butterfly fillets, liver, and abdominal fat pad were recorded. Left-side fillets were scored to obtain the degree of white striping for each bird. Representative samples for NORM (n = 24) and SEV (n = 17) categories were selected to compare the hematologic and serologic profiles. The SEV birds had greater (P white striping. The elevated serum enzyme levels confirm the muscle damage associated with the degenerative myopathy in SEV birds.

  4. The Effect of Losartan on Platelet Aggregation and Hematological Parameters in Patients with Newly Diagnosed Hypertension

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    Mustafa Ünübol

    2015-12-01

    Full Text Available Objective: Hypertension is associated with increased platelet function. Some antihypertensive drugs have antiplatelet activity. In this study, we aimed to investigate the effects of losartan on platelet aggregation induced by adenosine diphosphate (ADP, collagen, epinephrine, ristocetin, other hematological and, inflammatory parameters. Materials and Methods: Twenty-five patients (19 female, 6 male; mean age: 54±8 years with newly diagnosed hypertension were included in the study. All patients were with stage 1-2 essential hypertension according to the seventh report of the Joint National Committee on prevention, detection, evaluation, and treatment of high blood pressure. Initial blood pressure measurement was performed in all patients and losartan 100 mg/daily together with life style changes, such as diet and exercise was started. Platelet aggregation was evaluated with the use of ristocetin, epinephrine, collagen, and ADP. Complete blood count was also done. Platelet aggregation tests and blood pressure measurements were repeated after 8 weeks of therapy. Results: Systolic and diastolic blood pressure significantly decreased with losartan after 8 weeks (p0.05. The aggregation with ristocetin significantly decreased (p=0.027. Besides, significantly lower hemoglobin and hematocrit levels were observed (p=0.034, p=0.039, respectively. Conclusion: Losartan may produce independent activities apart from its antihypertensive effects by providing significant reductions in platelet aggregation with ristocetin, and in hematocrit levels with hemoglobin. Therefore, it may be beneficial in the prevention of atherosclerosis and thrombosis.

  5. Changes in Hematological, Biochemical and Non-specific Immune Parameters of Olive Flounder, , Following Starvation

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    Jong-Hyun Kim

    2014-09-01

    Full Text Available Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: 119.8±17.46 g, were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.

  6. Adhesion molecules expression in CLL: Potential impact on clinical and hematological parameters.

    Science.gov (United States)

    Kamel, Azza M; El-Sharkawy, Nahla M; Osman, Randa A; Abd El-Fattah, Eman K; El-Noshokaty, Essam; Abd El-Hamid, Thoraya; Kandeel, Eman Z

    2016-03-01

    B-cell chronic lymphocytic leukemia (CLL) is marked by the accumulation of CD5+ B lymphocytes within the blood, bone marrow (BM), and secondary lymphoid tissues. Abnormalities in the expression and function of cell adhesion molecules may account for the patterns of intra-nodal growth and hematogenous spread of the malignant cells. Chemokines and integrin-mediated adhesion and trans-endothelial migration (TEM) are central aspects in trafficking and retention of hematopoietic cells in the BM and lymphoid organs. This work was conducted to study adhesion molecules status in CLL and its potential impact on both hematological and clinical parameters. The study included 78 newly diagnosed CLL patients. Immunophenotyping was performed on peripheral blood using the chronic lymphoid panel. Adhesion molecules (CD11a, CD11b, CD49d, CD49C, CD29 and CD38) were tested using monoclonal antibodies and analyzed by Flow Cytometry. Positive correlation was encountered between adhesion molecules: CD38 with CD49d (r=0.25, p=0.028), CD11a with CD11b, CD49d and CD29 (r=0.394, p=0.001; r=0.441, p=molecules expression in CLL is apparently reflected on the potential migratory behavior of the leukemic cells to different organs. Copyright © 2016 National Cancer Institute, Cairo University. Production and hosting by Elsevier B.V. All rights reserved.

  7. Differentiating between dengue fever and malaria using hematological parameters in endemic areas of Thailand.

    Science.gov (United States)

    Kotepui, Manas; PhunPhuech, Bhukdee; Phiwklam, Nuoil; Uthaisar, Kwuntida

    2017-03-02

    Dengue fever (DF) and malaria are the two major public health concerns in tropical countries such as Thailand. Early differentiation between dengue and malaria could help clinicians to identify patients who should be closely monitored for signs of dengue hemorrhagic fever or severe malaria. This study aims to build knowledge on diagnostic markers that are used to discriminate between the infections, which frequently occur in malaria-endemic areas, such as the ones in Thailand. A retrospective study was conducted in Phop Phra Hospital, a hospital located in the Thailand-Burma border area, a malaria-endemic area, between 2013 and 2015. In brief, data on 336 patients infected with malaria were compared to data on 347 patients infected with DF. White blood cells, neutrophil, monocyte, eosinophil, neutrophil-lymphocyte ratio, and monocyte-lymphocyte ratio were significantly lower in patients with DF compared to patients with malaria (P dengue and malaria infection. This study concluded that several hematological parameters were different for diagnosing DF and malaria. A decision tree model revealed that using neutrophils, lymphocyte, MCHC, and gender was guided to discriminate patients with dengue and malaria infection. In addition, using these markers will thus lead to early detection, diagnosis, and prompt treatment of these tropical diseases.

  8. Effects of Del. Stem Bark Extract on Hematological and Biochemical Parameters of Wistar Rats

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    Moses Z. Zaruwa

    2016-01-01

    Full Text Available Africa is rich in a wide range of flora that are exploited as herbal medicines and remedies. Several diseases such as diabetes, diarrhea, dysentery and jaundice have been successfully managed using herbal medicines. Herbal decoctions or concoctions have been used as pain killers, antibiotics, and hematinics. This study evaluated the hematopoietic and biochemical properties of the stem bark of Sterculia setigera Del. in Wistar rats. Results showed that S. setigera decoction has copiously high tannin and cardiac glycoside levels. Ingestion of the decoction by rats over a 16-day period significantly ( P < 0.05 increased the body weights of rats by 22.4% in the S. setigera -treated group. Hematological profiles showed raised levels of red blood cells, hemoglobin, packed cell volume, mean corpuscular volume, mean cell hemoglobin, mean cell hemoglobin concentration, and platelets, while biochemical parameters showed lower levels of alanine aminotransferase and aspartate aminotransferase, and slight increase in albumin and TP levels. We posit that the results justify the use of the stem bark of S. setigera as a hematinic by traditional medical practitioners and show its relative safety. Further experiments are needed to evaluate its safety.

  9. Combination of change in hematological parameters with exercise stress test to predict coronary artery disease.

    Science.gov (United States)

    Korkmaz, Ahmet; Yıldız, Abdulkadir; Türker Duyuler, Pınar; Duyuler, Serkan; Yılmaz, Samet; Basyigit, Funda; Elalmis, Ozgul Ucar; Guray, Umit; Ileri, Mehmet

    2018-01-01

    Treadmill exercise stress testing for identifying patients with a higher likelihood of coronary artery disease (CAD) before elective coronary angiography is recommended in the current guidelines. In this study, we aimed to evaluate the changes in the hematological parameters before and after exercise stress test in relation with the presence of CAD. A total of 113 patients with chest pain who underwent treadmill exercise testing and coronary angiography were included in this study. Neutrophil count (4.38±0.99 vs 5.19±0.93, Pexercise test in all the patients. Increase in the NLR after exercise test was significantly higher in patients with positive exercise test (n=68) than negative exercise test (n=45) (0.49±0.58 vs 0.19±0.44, P=.016). The sensitivity and specificity of treadmill exercise testing according to coronary angiography was 79% and 64%, respectively. A cut-off point of 0.2 for the change in the NLR in addition to positive treadmill exercise testing had 91% sensitivity and 92% specificity in predicting significant coronary artery stenosis (AUC:0.913, 95% CI: 0.805-1.000, P<.001). Neutrophil to lymphocyte ratio is an important inflammatory marker that can contribute to treadmill ECG testing in predicting CAD. © 2017 Wiley Periodicals, Inc.

  10. Antigen Expression on Blast Cells and Hematological Parameters at Presentation in Acute Lymphoblastic Leukemia Patients

    International Nuclear Information System (INIS)

    Naeem, S.; Bukhari, M. H.

    2015-01-01

    Objective: To analyze the expression of various antigens on the leukemic blasts and to determine the hematological parameters, in Acute Lymphoblastic Leukemia (ALL) patients at presentation. Study Design: Observational study. Place and Duration of Study: King Edward Medical University, Lahore and Hameed Latif Hospital, Lahore, from February 2013 to March 2014. Methodology: A total of 50 newly diagnosed and untreated patients of ALL were selected from Mayo Hospital and Hameed Latif Hospital. These patients included both genders and all age groups. Hemoglobin, total leukocyte count and platelet count were determined on hematology analyser-Sysmex-Kx-2I. Blast cell percentage was estimated on Giemsa stained blood smears. Immuno phenotyping was done on bone marrow samples by 5 colour flow cytometery on Beckman Counter Navious Flow cytometer. An acute leukemia panel of 23 antibodies was used. The data was entered and analyzed in SPSS version 22. Results: Of the 50 ALL patients, 36 (72 percentage) were B-ALL and 14 (28 percentage) T-ALL. There were 18 (36 percentage) children and 32 (64 percentage) adults. T-ALL included 22 percentage of the childhood and 31 percentage of the adult cases. Immuno phenotypic analysis showed that CD19, CD79a and CD20 were B-lineage specific markers whereas cCD3, CD3 and CD5 were T-lineage specific. CD10 was the most sensitive marker for B-ALL and CD7 was the most sensitive marker of T-ALL. TdT was expressed in 92 percentage B-ALL and 71 percentage T-ALL cases, CD34 in 58 percentage and 43 percentage cases and CD45 in 83 percentage and 100 percentage respectively. High leukocyte count (> 50 x 109/L) was present in 58 percentage cases. Hemoglobin was < 10 g/dl in 74 percentage patients and platelet count was below 20 x 109/Lin 12 percentage patients. Leukocyte count, hemoglobin, platelet count and blast cell percentage did not show a significant difference in the two ALL immuno types. Conclusion: The frequency of T-ALL is higher in childhood

  11. Effect of Withania somnifera Extracts on Some Selective Biochemical, Hematological, and Immunological Parameters in Guinea Pigs Experimental Infected with E. coli

    Science.gov (United States)

    El-Boshy, Mohamed El-Sayed; Abdalla, Osama Mohamed; Risha, Angy; Moustafa, Fatma

    2013-01-01

    Fifty 1-2-month-old Guinea pigs were divided into 5 equal groups, 10 each. Control (Gp1) did receive neither viable bacteria nor treatment. Each animal from the other groups (Gp2–5) was challenged with (1-2 × 108) viable E. coli in 200 μL normal saline (0.9%) through IP route. GP2 infected group was treated with 200 μL saline IP and kept as positive control group. Gp3-4 are infected and treated with Withania somnifera (ethanol root extract) with doses 50 and 100 mg/kg. BW, respectively. Gp5 infected treated group was treated with cefoperazone antibiotic at dose 35 mg/Kg BW. The treatment by drug or the extracted medicinal plant was started 72 h after infection for 7 successive days. Serum and whole blood sample were collected from all groups 14 days after treatment to evaluate some hematological and biochemical changes as well as immunomodulatory cytokine tumor necrosis factor-alpha (TNF-α). Oral treatment of the plant extract caused significant benefit results in infected Guinea pig appeared in the correction of some hematological and biochemical parameters also try to suppressed inflammatory cytokine response represent in TNF-α. It could be concluded that W. somnifera extract has potent antibacterial activity, and this appears in the correction with hematological, biochemical, and immunological results. PMID:23738137

  12. Hematological parameters in sick cell anemia patients with and without priapism

    International Nuclear Information System (INIS)

    Ahmed, Sagir G.; Ibrahim, Umma A.; Hassan, Abba W.

    2006-01-01

    Priapism was associated with certain hematological parameters in sickle cell anemia (SCA) patients in one report but not in another. We studied differences haematological parameters between SCA patients with and without priapism. Eighteen patients with SCA who presented with acute priapism during the years 2001-2004 were compared with age-and sex-matched SCA patients without priapism with respect to hematocrit, reticulocyte count, level of irreversibly sickled cells (ISC), percentage of haemoglobin (Hb F), total leukocyte and platelet counts. SCA patients with priapism had a mean hematocrit of 0.28 L/L, which was significantly higher than mean hematocrit value of 0.24 L/L (P<0.05) in patients without priapism. The mean reticulocyte count of 8% in patients with priapism was siginificantly lower than mean reticulocyte count of 12% (P<0.05) in patients without priapism. The level of ISC of 3% in patients with priapism was significantly lower than the level of 6.5% (P<0.05) in patients without priapism. There was no statistically significant difference in the mean levels of Hb F (7% vs. 6%). Patients with priapism had a mean leukocyte count that did not significantly differ from values in patients without priapism. SCA patients with priapism had a lower rate of hemolysis resulting in a higher hematocrit and greater blood viscosity, which increased the risk of corpora cavernosal sickling and blockade. Hence, a relatively higher hematocrit is risk factor for the development priapism in patients with sickle cell anemia. (author)

  13. Clinical, Toxicological, Biochemical, and Hematologic Parameters in Lead Exposed Workers of a Car Battery Industry

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    Sina Kianoush

    2013-03-01

    Full Text Available Background: Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Methods: Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto Analyzer; Model BT3000 and hematologic (using Cell Counter Sysmex; Model KX21N parameters. An atomic absorption spectrometer (Perkin-Elmer, Model 3030, USA was used to determine lead concentration in blood and urine by heated graphite atomization technique. Results: A total of 112 men mean age 28.78±5.17 years, who worked in a car battery industry were recruited in the present study. The most common signs/symptoms of lead poisoning included increased excitability 41.9%, arthralgia 41.0%, fatigue 40.1%, dental grey discoloration 44.6%, lead line 24.1%, increased deep tendon reflexes (DTR 22.3%, and decreased DTR (18.7%. Blood lead concentration (BLC was 398.95 µg/L±177.40, which was significantly correlated with duration of work (P=0.044 but not with the clinical manifestations of lead poisoning. However, BLC was significantly correlated with urine lead concentration (83.67 µg/L±49.78; r2=0.711; P<0.001, mean corpuscular hemoglobin (r=-0.280; P=0.011, mean corpuscular hemoglobin concentration (r=-0.304; P=0.006 and fasting blood sugar or FBS (r=-0.258; P=0.010. Conclusion: Neuropsychiatric and skeletal findings were common manifestations of chronic occupational lead poisoning. BLC was significantly correlated with duration of work, urine lead concentration, two hemoglobin indices and FBS.

  14. Effects of Graded Levels of Montmorillonite on Performance, Hematological Parameters and Bone Mineralization in Weaned Pigs

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    Q. W. Duan

    2013-11-01

    Full Text Available The aim of this study was to investigate the effects of graded levels of montmorillonite, a constituent of clay, on performance, hematological parameters and bone mineralization in weaned pigs. One hundred and twenty, 35-d-old crossbred pigs (Duroc×Large White×Landrace, 10.50±1.20 kg were used in a 28-d experiment and fed either an unsupplemented corn-soybean meal basal diet or similar diets supplemented with 0.5, 1.0, 2.5 or 5.0% montmorillonite added at the expense of wheat bran. Each treatment was replicated six times with four pigs (two barrows and two gilts per replicate. Feed intake declined (linear and quadratic effect, p< 0.01 with increasing level of montmorillonite while feed conversion was improved (linear and quadratic effect, p<0.01. Daily gain was unaffected by dietary treatment. Plasma myeloperoxidase declined linearly (p = 0.03 with increasing dietary level of montmorillonite. Plasma malondialdehyde and nitric oxide levels were quadratically affected (p<0.01 by montmorillonite with increases observed for pigs fed the 0.5 and 1.0% levels which then declined for pigs fed the 2.5 and 5.0% treatments. In bone, the content of potassium, sodium, copper, iron, manganese and magnesium were decreased (linear and quadratic effect, p<0.01 in response to an increase of dietary montmorillonite. These results suggest that dietary inclusion of montmorillonite at levels as high as 5.0% does not result in overt toxicity but could induce potential oxidative damage and reduce bone mineralization in pigs.

  15. State of the art vs biological variability: Comparison on hematology parameters using Spanish EQAS data.

    Science.gov (United States)

    Molina, A; Guiñon, L; Perez, A; Segurana, A; Bedini, J L; Reverter, J C; Merino, A

    2018-02-05

    It is important for clinical laboratories to maintain under control the possible sources of error in its analytical determinations. The objective of this work is to perform an analysis of the total error committed by laboratories using the data extracted from the Spanish External Quality Assessment Program in Hematology and to compare them with the specifications based on the biological variability proposed by the Ricós group. We analyzed a total of 3 89 000 results during the period 2015-2016 from the following quantitative schemes of Spanish External Quality Assessment Program: complete blood count, blood coagulation tests, differential leukocyte count, reticulocytes, hemoglobin A 2 , antithrombin, factor VIII, protein C, and von Willebrand factor. It has been considered as an indicator of the current performance the value of total error that 90% of laboratories are able to achieve, taking into account 75% of their results. We found some magnitudes whose biological variability specifications are achievable by most of the laboratories for either minimum, desirable, or optimum criteria: white blood cells, red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, platelets, fibrinogen, neutrophils, lymphocytes, eosinophils, von Willebrand factor, and protein C. However, current performance for mean corpuscular hemoglobin concentration and hemoglobin A 2 only allows to meet the specifications based on the state of the art. Our results reflect the feasibility of establishing specifications based on biological variability criteria or the state of the art, which may help to select the proper criteria for each parameter. © 2018 John Wiley & Sons Ltd.

  16. Clinical, toxicological, biochemical, and hematologic parameters in lead exposed workers of a car battery industry.

    Science.gov (United States)

    Kianoush, Sina; Balali-Mood, Mahdi; Mousavi, Seyed Reza; Shakeri, Mohammad Taghi; Dadpour, Bita; Moradi, Valiollah; Sadeghi, Mahmoud

    2013-03-01

    Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto Analyzer; Model BT3000) and hematologic (using Cell Counter Sysmex; Model KX21N) parameters. An atomic absorption spectrometer (Perkin-Elmer, Model 3030, USA) was used to determine lead concentration in blood and urine by heated graphite atomization technique. A total of 112 men mean age 28.78±5.17 years, who worked in a car battery industry were recruited in the present study. The most common signs/symptoms of lead poisoning included increased excitability 41.9%, arthralgia 41.0%, fatigue 40.1%, dental grey discoloration 44.6%, lead line 24.1%, increased deep tendon reflexes (DTR) 22.3%, and decreased DTR (18.7%). Blood lead concentration (BLC) was 398.95 µg/L±177.40, which was significantly correlated with duration of work (P=0.044) but not with the clinical manifestations of lead poisoning. However, BLC was significantly correlated with urine lead concentration (83.67 µg/L±49.78; r(2)=0.711; P<0.001), mean corpuscular hemoglobin (r=-0.280; P=0.011), mean corpuscular hemoglobin concentration (r=-0.304; P=0.006) and fasting blood sugar or FBS (r=-0.258; P=0.010). Neuropsychiatric and skeletal findings were common manifestations of chronic occupational lead poisoning. BLC was significantly correlated with duration of work, urine lead concentration, two hemoglobin indices and FBS.

  17. Some hematological parameters of Wistar rats treated with Chromolaena odorata leave extracts

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    Henshaw Uchechi Okoroiwu

    2017-10-01

    Full Text Available This study was designed to investigate the effects of the different extracts of Chromolaena odorata leave on the hematopoietic system of Wistar rats. Solvent extraction was used for the ethanol and aqueous extractions while decoction method was used for the crude extraction. Fifty Wistar rats of both sexes weighing 140-180 g were used for this study. They were divided into ten groups each containing five rats. The animals were fed the extracts by oral gavage once daily for 21 days. Blood sample was collected via cardiac artery. Hematological parameters were analyzed using automation method. The ethanol extract gave the highest extract yield. The aqueous, ethanol and crude extraction had median lethal toxicity (LD50 of 2738.6 mg/kg, 1581.1 mg/kg and 224.7 mg/kg, respectively. Significant difference (P<0.05 in the total white blood cell count was observed in the 75 mg/kg ethanol and 300 mg/kg crude extracts when compared with control group. Significant difference (P<0.05 in the hemoglobin concentration was observed in the 150 mg/kg ethanol extracts when compared with the control group. Significant difference (P<0.05 in the packed cell volume was seen in the 75 mg/kg aqueous, 150 mg/kg aqueous and 75 mg/kg ethanol extracts in respect to the control group. The mean cell volume, the mean platelet volume and platelet large cell ratio of the 75 mg/kg aqueous extract were significantly different (P<0.05 when compared with the control group. The present study showed possible treatment-induced hematopoietic function of C. odorata leave extracts.

  18. HEMATOLOGICAL BLOOD PARAMETERS OF YOUNG-OF-THE-YEAR CARPS (CYPRINIDAE REARED USING FISH RANCHING TECHNOLOGY IN THE SOUTHERN UKRAINE

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    Yu. Volichenko

    2015-12-01

    Full Text Available Purpose. To investigate the hematological characteristics of blood young-of-the-year carps reared using fish ranching technology in the southern Ukraine and to establish certain correlations between the main fish technical features of the studied groups of the young-of-the-year fish. Methodology. The studies were based on field and experimental methods adopted for fisheries, biochemical and statistical studies. Findings. The paper contains the data on mean fish weight, hematological and biochemical parameters of serum of young-of-the-year carps reared using fish ranching technology. Based on a comparative analysis of the obtained data, we detected significant peculiarities and qualitative difference in the absence of foam cells and basophils, which distinguish carp from the group of herbivorous fish, established significant correlations between mean fish weight and hematologic indices of blood. Marked by fish-breeding relationships with signs like weight and some parameters of blood components in all studied fish: hemoglobin in the range from 0.7858 to 0.9943, number of erythrocytes from 0.7843 to 0.9942, lymphocytes from 0.7848 to 0.9949, сholеsterol from 0.7640 to 0.9616 and triglycerides of 0.7499 in silver carp (Hypophthalmichthys molitrix and 0.9616 in common carp (Cyprinus carpio carpio. To show these relationships graphically, we used regression analysis and the obtained regression equations can give an accurate assessment of the quality of fish seeds through hematological blood parameters and mean weight. Originality. The analysis of hematological parameters of blood of young-of-the-year carps: common carp (Cyprinus carpio carpio, silver carp (Hypophthalmichthys molitrix, bighead carp (Hypophthalmichthys nobilis, grass carp (Ctenopharyngodon idella reared using fish ranching technology in the south of Ukraine was performed for the first time. Practical value. The obtained data allow scientifically recommending them as a component of

  19. Nondestructive discrimination between normal and hematological malignancy cell lines using near-infrared Raman spectroscopy and multivariate analysis

    Science.gov (United States)

    Huang, Hao; Lin, Duo; Chen, Weiwei; Yu, Yun; Xu, Jijin; Liang, Zhen; Lin, Xi; Dong, Zhong; Shi, Hong

    2014-08-01

    An accurate understanding of biomolecular changes in living cells associated with malignant transformation is of paramount importance in early cancer detection. The aim of this study was to apply near-infrared Raman spectroscopy (RS) for differentiating cancer from normal cells. High-quality Raman spectra in the range of 450-1800 cm-1 can be obtained from 31 normal and 64 hematological malignancy cells including 43 CA46 and 21 U266 cells. There were significant differences in Raman spectra between normal and cancer groups, which suggests special changes in the percentage of biomolecules including lipid, nucleic acids and proteins in different cell lines. A diagnostic accuracy of 100% can be achieved by principal components analysis (PCA) combined with linear discriminant analysis (LDA) for classification between cancer and normal cell lines. This exploratory study demonstrates the potential application of the RS technique combined with PCA-LDA as a clinical cell-based biosensor for the noninvasive cancer detection and screening at the molecular level.

  20. The association between hematological parameters and insulin resistance is modified by body mass index - results from the North-East Italy MoMa population study.

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    Rocco Barazzoni

    Full Text Available OBJECTIVE: Increments in red blood cell count (RBC, hemoglobin (Hb and hematocrit (Ht levels are reportedly associated with higher insulin resistance (IR. Obesity may cause IR, but underlying factors remain incompletely defined, and interactions between obesity, hematological parameters and IR are incompletely understood. We therefore determined whether: 1 BMI and obesity per se are independently associated with higher RBC, hemoglobin and hematocrit; 2 hematological parameters independently predict insulin resistance in obese individuals. DESIGN AND METHODS: We investigated the associations between BMI, hematological parameters and insulin resistance as reflected by homeostasis model assessment (HOMA in a general population cohort from the North-East Italy MoMa epidemiological study (M/F = 865/971, age = 49 ± 1. RESULTS: In all subjects, age-, sex- and smoking-adjusted hematological parameters were positively associated with BMI in linear regression (P<0.05, but not after adjustment for HOMA or waist circumference (WC and potential metabolic confounders. No associations were found between hematological parameters and BMI in lean, overweight or obese subgroups. Associations between hematological parameters and HOMA were conversely independent of BMI in all subjects and in lean and overweight subgroups (P<0.01, but not in obese subjects alone. CONCLUSIONS: In a North-East Italy general population cohort, obesity per se is not independently associated with altered RBC, Hb and Ht, and the association between BMI and hematological parameters is mediated by their associations with abdominal fat and insulin resistance markers. High hematological parameters could contribute to identify insulin resistance in non-obese individual, but they do not appear to be reliable insulin resistance biomarkers in obese subjects.

  1. Impact of malaria on hematological parameters in people living with HIV/AIDS attending the Laquintinie Hospital in Douala, Cameroon.

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    Gervais Gouana Tchinda

    Full Text Available BACKGROUND: People living with HIV/AIDS (PLWHA frequently have abnormal blood counts including anemia, leucopenia and thrombocytopenia. The role of infection with plasmodia on these hematological parameters in PLWHA is not well known. In this study we compared selected hematological parameters between malaria positive and negative PLWHA. METHODS: We conducted a cross-sectional study of PLWHA attending the Douala Laquintinie hospital. After obtaining consent, demographic and clinical data were obtained via a standardized questionnaire. Blood samples collected for hematological assays were run using an automated full blood counter. Malaria parasitaemia was determined by blood smear microscopy. RESULTS: A total of 238 adult PLWHA were enrolled, 48.3% of who were on antiretroviral therapy and 24.8% of whom had malaria parasitaemia. The respective mean (±SD of hemoglobin level, RBC count, WBC count, platelet count, lymphocyte count and CD4+ T cell counts in malaria co-infected patients versus non-infected patients were: 10.8(±1.9 g/dl versus 11.4(±2.0 g/dl; 3,745,254(±793,353 cells/µl versus 3,888,966(±648,195 cells/µl; 4,403(±1,534 cells/µl versus 4,920(±1,922 cells/µl; 216,051(±93,884 cells/µl versus 226,792(±98,664 cells/µl; 1,846(±711 cells/µl versus 2,052(±845 cells/µl and 245(±195 cells/µl versus 301(±211 cells/µl. All these means were not statistically significantly different from each other. CONCLUSION: There was no significant difference in studied hematological parameters between malaria positive and negative PLWHA. These data suggest little or no impact of malaria infection. Hematological anomalies in PLWHA in this area need not be necessarily attributed to malaria. These need to be further investigated to identify and treat other potential causes.

  2. Hematological Parameters and Carcass Characteristics of Weanling Rabbits Fed Sesame Seed Meal (Sesamum indicum in a Semi-Arid Region

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    A. A. Njidda* and C. E. Isidahomen1

    2011-01-01

    Full Text Available The effect of replacing groundnut cake with Sesame seed meal (Sesamum indicum on hematology, blood chemistry and carcass characteristics was evaluated in rabbits. Sixteen (New Zealand white rabbits were randomly assigned to four dietary treatments in a completely randomized design with four rabbits per treatment. The rabbits were fed diets containing 0, 4, 8 and 12% of sesame seed meal (SSM and designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were selected for hematological analysis and carcass evaluation. There were significant differences (P0.05 by the levels of SSM in the diets. There were significant differences (P0.05 on serum albumin and total proteins among treatments. The carcass characteristics also showed significant differences (P0.05 on the hematological parameters, serum biochemical indices and carcass characteristics.

  3. Hematological parameters on the effect of the jellyfish venom Cassiopea andromeda in animal models

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    Iraj Nabipour

    2017-04-01

    Full Text Available For the first time, we previously recorded an enormous population of the Cassiopea andromeda jellyfish that had increased dramatically from Bushehr coasts of Iran. The sub-acute toxicity of the jellyfish venom in rat organs was correspondingly carried out. The data presented in this paper relate to the in vivo and in vitro hematological effects of this venomous species of jellyfish venom.

  4. Score Normalization using Logistic Regression with Expected Parameters

    NARCIS (Netherlands)

    Aly, Robin

    State-of-the-art score normalization methods use generative models that rely on sometimes unrealistic assumptions. We propose a novel parameter estimation method for score normalization based on logistic regression. Experiments on the Gov2 and CluewebA collection indicate that our method is

  5. New parameters available on Sysmex XE-5000 hematology analyzers contribute to differentiating dengue from leptospirosis and enteric fever.

    Science.gov (United States)

    Oehadian, A; Michels, M; de Mast, Q; Prihatni, D; Puspita, M; Hartantri, Y; Sinarta, S; van der Ven, A J A M; Alisjahbana, B

    2015-12-01

    Distinguishing dengue virus infection from other febrile thrombocytopenic illnesses such as leptospirosis or enteric fever is important but difficult, due to the unavailability of reliable diagnostic tests. Sysmex XE-5000 hematology analyzers use fluorescence flow cytometry to quantitate new parameters including cells in the atypical lymphocyte area (AL), high-fluorescent lymphocyte counts (HFLC), immature granulocytes (IG), and immature platelets (IPF). This study aimed to investigate whether these parameters can help to discriminate between the diseases. We compared hematocytometry performed by a Sysmex XE-5000 analyzer in Indonesian adults with dengue (n = 93), leptospirosis (n = 11), and enteric fever (n = 6) infection, and in healthy controls (n = 28). Receiver operating characteristic curves comparing dengue and leptospirosis showed that dengue was characterized by increased %AL (AUC 0.87; 95% CI 0.70-1.03), %HFLC (AUC 0.89; 95% CI 0.78-0.99), and %IPF (AUC 0.81; 95% CI 0.65-0.97), while patients with leptospirosis had increased %IG (AUC 0.86; 95% CI 0.71-1.02). Low %AL, %HFLC, and %IG supported a diagnosis of enteric fever. The detection of AL, HFLC, IG, and IPF by Sysmex XE-5000 hematology analyzers can help to differentiate between common causes of febrile illnesses with thrombocytopenia in dengue endemic areas. We recommend further investigating the discriminatory value of these parameters in clinical practice. © 2015 John Wiley & Sons Ltd.

  6. Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish.

    Science.gov (United States)

    Ribas, João Luiz Coelho; Zampronio, Aleksander R; Silva de Assis, Helena C

    2016-04-01

    The aim of the present study was to evaluate the effects of diclofenac and dexamethasone on hematological parameters and immune response in the fish species Hoplias malabaricus after trophic exposure. Fish were fed twice every week with Astyanax sp., which were given an intraperitoneal inoculation with diclofenac (0 μg/kg, 0.2 μg/kg, 2.0 μg/kg, or 20.0 μg/kg) or dexamethasone (0.03 μg/kg, 0.3 μg/kg, or 3.0 μg/kg). After 12 doses, the hematological parameters and lipopolysaccharide-induced nitric oxide production by head kidney monocytic lineage were evaluated. Exposed fish also received 1 mg/kg of carrageenan intraperitoneal, and cell migration to the peritoneal cavity was evaluated after 4 h. Diclofenac and dexamethasone altered the red blood cell count, as well as hematocrit and hemoglobin levels. The total blood leukocyte count decreased in all groups. A significantly reduced carrageenan-induced leukocyte migration to the peritoneal cavity, particularly of polymorphonuclear cells, was observed at all tested doses, suggesting a possible immunosuppressive effect. The basal nitric oxide synthesis of head kidney cell cultures was reduced at the highest dose of diclofenac and was increased at the highest dose of dexamethasone. The lipopolysaccharide-stimulated nitric oxide production was reduced in all treatments, thus corroborating the immunosuppressive effect. Although some fish responses were variable for different drugs, the results suggested that trophic exposure to diclofenac and dexamethasone can lead to hematological changes and immunotoxic effects, causing negative impacts in aquatic organisms. © 2015 SETAC.

  7. Evaluation of Hematological Parameters in Partial Exchange and Packed Cell Transfusion in Treatment of Severe Anemia in Pregnancy

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    Sudha Salhan

    2012-01-01

    Full Text Available Objectives. Anemia is a major public health problem throughout the world which assumes prominence in pregnant mothers. Patients with severe anemia continue to present themselves at term or in labor. This study was conducted to compare the improvements in hematological parameters of patients receiving partial exchange blood transfusion and transfusion of packed cells without exchange. Methods. One hundred and twenty-five severely anemic antenatal mothers were admitted from outpatient service. Partial exchange transfusion was given to sixty-six patients while fifty-nine received transfusion of packed cells with frusemide cover. Results. The two groups were comparable in terms of age, height, weight, religion, diet, education, occupation of self and husband, and income. Hemoglobin level in Group 1 was comparatively less than Group 2 at prelevel (5.2±1.5 versus 6.6±2.3, P=0.001 and postlevel (7.2±1.5 versus 8.6±1.8, P=0.001, respectively, but there was no significant difference between the two modes of transfusion (2.09±1.6 versus 2.01±1.5, P=0.78. Conclusion. The study produced an equally significant improvement in hematological parameters in partial exchange and packed cell transfusion. Platelet counts were significantly less in partial exchange as compared with packed cell transfusion.

  8. Toxicological evaluations of Stigma maydis (corn silk aqueous extract on hematological and lipid parameters in Wistar rats

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    Sabiu Saheed

    2015-01-01

    Full Text Available Despite the acclaimed phytotherapeutic attributes of Stigma maydis in folkloric medicine, there is paucity of information on its toxicity profile on hematological and lipid parameters. The toxicological effect of aqueous extract of corn silk at 100, 200 and 400 mg/kg body weight on hematological indices in Wistar rats were evaluated progressively at 24 h after 1, 7, 14, 21 and 28 days. Lipid parameters were also analyzed at the end of the experimental period. We observed that the extract did not exhibit any significant (p > 0.05 effect on red blood cells, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and mean platelet volume at all the tested doses. The study however showed a significant increase in the serum levels of white blood cell, platelet, lymphocytes, high-density lipoprotein cholesterol; as well as feeding pattern in the animals, while the concentrations of total cholesterol, low-density lipoprotein cholesterol, and artherogenic index value were significantly lowered. These findings are suggestive of non-hematotoxic potential of the extract. Overall, the effect exhibited by corn silk extract in this study proved that, it is unlikely to be hematotoxic and could be a good candidature in the management of coronary heart diseases if consumed at the doses investigated.

  9. The effects of protein dietary supplementation on fecal egg counts and hematological parameters in goat kids with subclinical nematodosis.

    Science.gov (United States)

    Konwar, Priyanka; Tiwari, S P; Gohain, M; Kumari, Kiran

    2015-11-01

    The aim of the present study was to assess the effect of dietary supplementation with different levels of protein on fecal egg counts and hematological parameters in goat kids with subclinical nematodosis under semi-intensive condition. 20 goat kids (3-5 months old with an average body weight of 8.90 kg) were randomly allocated to four groups: T1, served as a negative control, without receiving concentrate feed, and T2, T3, and T4 that received concentrate feed containing 16, 20, and 24% digestible crude protein, respectively. The experiment was carried out for 60 days. In this study, protein supplementation had a significant (psupplementation, whereas monocyte (%) and neutrophil (%) values were not significantly influenced by protein supplementation effect during the entire experiment. The values of mean corpuscular volume (fl) were affected significantly (pprotein supplementation after 30 days, mean corpuscular Hb (MCH) (pg) after 45 days, but MCH concentration (g/dl) was not significantly different among the experimental groups during the entire experiment. The dietary supplementation with different levels of protein significantly improved the hematological profiles and inhibited the nematodosis infection in the experimental goat kids.

  10. Additive toxic effect of deltamethrin and cadmium on hepatic, hematological, and immunological parameters in mice.

    Science.gov (United States)

    Rehman, Hasibur; Aziz, Al Thbiani; Saggu, Shalini; VanWert, Adam L; Zidan, Nahla; Saggu, Sundeep

    2017-06-01

    Exposure to natural and man-made environmental toxins concurrently can pose a greater threat to multiple organs. In the present work, we investigated interactions between deltamethrin (DM) and cadmium (Cd), whose mechanisms of action in humans are poorly understood. Albino mice were randomly divided into four groups, each containing six mice: saline as control, DM-treated, cadmium chloride (CdCl 2 )-treated, and CdCl 2 plus DM treated. After 2 weeks of treatment biochemical and hematological effects, total leukocyte count (TLC), differential leukocyte count, humoral-mediated immune responses, and histopathological studies were conducted. Mice exposed to DM and Cd showed a significant increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Also, DM and Cd administration resulted in suppression of humoral immunity, erythrocyte count, hemoglobin, hematocrit, and TLC. Histopathological evidence revealed hepatic damage, supporting the AST and ALT findings. Cd and DM exhibited an additive type of toxicity. It could be concluded that these toxins either target different cellular pathways, or the individual amounts used in this study were not enough to saturate the toxicological target, thus producing additive effects.

  11. Changes in Hematological, Biochemical and Non-specific Immune Parameters of Olive Flounder, Paralichthys olivaceus, Following Starvation.

    Science.gov (United States)

    Kim, Jong-Hyun; Jeong, Min Hwan; Jun, Je-Cheon; Kim, Tae-Ik

    2014-09-01

    Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: 119.8±17.46 g), were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months) starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.

  12. Combined effect of workplace noise and smoking on some hematological parameters on workers in a food manufacturing plant.

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    I. Alimohammadi

    2014-10-01

    Full Text Available Generally, no industry can be found to be safe in terms of noise pollution. Noise is the wide- spread form of environmental stressor in the industrialized urban areas. Aim: the purpose of this paper is to evaluate the combined effect of workplace noise and smoking on some hematological parameters on employing work in a food manufacturing plant. This is a retrospective study before and after for five years since starting of the work. In this study, 50 male subjects participated: smokers (n=11 and nonsmokers (n=39, for further individual information and certain specific points, a developed standard questionnaire, were filled out by participants. For the past 4-year’s details, blood tests and medical records of persons since initially hired, were used. The details of the fifth year were measured by the presenters. Using the ISO protocol 1999 and 9612, workplace noise was measured and the noise map was drawn using arc-view GIS software. Statistical analysis SPSS software version 18 was investigated. Due to the nature of the study, the significance level was set at a P value ≤0.1. Statistical findings and laboratory data showed that the effect of noise and smoking on red blood cells and white blood cells of smokers and nonsmokers was significant (p<0.1, so that the amount of red blood cells in smokers who are exposed to noise exceeding 88.83 dB, is higher than nonsmokers, and the white blood cells are lower in nonsmokers in compared with smokers. Our findings showed that combined of workplace noise and smoking has severe adverse effects on hematological parameters, and these alterations might be associated with a greater risk for more diseases. It is notable that results are from a research effort of its researchers and it is not completely certain so further investigation will be needed.

  13. Intestinal microbial ecology and hematological parameters of broiler fed cassava waste pulp fermented with Acremonium charticola

    Science.gov (United States)

    Sugiharto, Sugiharto; Yudiarti, Turrini; Isroli, Isroli; Widiastuti, Endang; Putra, Fatan Dwi

    2017-01-01

    Aim: The aim of this study was to evaluate the effect of feeding Acremonium charticola-fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens. Materials and Methods: A total of 240 male Lohman day-old-chicks were randomly allotted to one of the four experimental diets including control diet, control diet + antimicrobials (neomycin; 300 mg/kg diet), diet containing AC-FCP (16 g/100 g diet) or diet containing AC-FCP + antimicrobials. At day 28, the birds from each pen were blood sampled, sacrificed and immediately the internal organs were removed and weighed. Digesta were obtained from the ileum and cecum. Results: Birds fed AC-FCP had lower (pButiric acid was higher (p<0.05) in the cecal content of birds fed AC-FCP than in other birds. Propionic acid was also higher in AC-FCP fed birds than in other birds although statistically not significant. The percentages of lymphocytes and heterophils were higher (p<0.05) and tended (p=0.07) to be lower, respectively, in broilers fed control diet than in other birds. The birds provided control diet had lower (p<0.05) heterophils to lymphocytes ratio compared to those receiving AC-FCP or AC-FCP + antimicrobials. Serum total protein and globulin were higher (p<0.01) in birds fed control diet or control diet + antimicrobials compared to AC-FCP or AC-FCP + antimicrobials fed birds. Serum albumin was lower (p<0.01) in AC-FCP birds than that in other birds. There was a tendency (p=0.09) that birds fed AC-FCP diet had lower total serum cholesterol than other birds. Conclusion: Feeding AC-FCP has potential to improve the intestinal health and protect the birds from acute infections. PMID:28435195

  14. Impact of Chemotherapy on Normal Tissue Complication Probability Models of Acute Hematologic Toxicity in Patients Receiving Pelvic Intensity Modulated Radiation Therapy

    Energy Technology Data Exchange (ETDEWEB)

    Bazan, Jose G.; Luxton, Gary; Kozak, Margaret M.; Anderson, Eric M.; Hancock, Steven L.; Kapp, Daniel S.; Kidd, Elizabeth A.; Koong, Albert C.; Chang, Daniel T., E-mail: dtchang@stanford.edu

    2013-12-01

    Purpose: To determine how chemotherapy agents affect radiation dose parameters that correlate with acute hematologic toxicity (HT) in patients treated with pelvic intensity modulated radiation therapy (P-IMRT) and concurrent chemotherapy. Methods and Materials: We assessed HT in 141 patients who received P-IMRT for anal, gynecologic, rectal, or prostate cancers, 95 of whom received concurrent chemotherapy. Patients were separated into 4 groups: mitomycin (MMC) + 5-fluorouracil (5FU, 37 of 141), platinum ± 5FU (Cis, 32 of 141), 5FU (26 of 141), and P-IMRT alone (46 of 141). The pelvic bone was contoured as a surrogate for pelvic bone marrow (PBM) and divided into subsites: ilium, lower pelvis, and lumbosacral spine (LSS). The volumes of each region receiving 5-40 Gy were calculated. The endpoint for HT was grade ≥3 (HT3+) leukopenia, neutropenia or thrombocytopenia. Normal tissue complication probability was calculated using the Lyman-Kutcher-Burman model. Logistic regression was used to analyze association between HT3+ and dosimetric parameters. Results: Twenty-six patients experienced HT3+: 10 of 37 (27%) MMC, 14 of 32 (44%) Cis, 2 of 26 (8%) 5FU, and 0 of 46 P-IMRT. PBM dosimetric parameters were correlated with HT3+ in the MMC group but not in the Cis group. LSS dosimetric parameters were well correlated with HT3+ in both the MMC and Cis groups. Constrained optimization (0normal tissue complication probability curve compared with treatment with Cis. Dose tolerance of PBM and the LSS subsite may be lower for

  15. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits.

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Ni, Hengjia; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu; Fang, Jun

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes . On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved ( P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased ( P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower ( P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased ( P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  16. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Directory of Open Access Journals (Sweden)

    Maidelys Iser

    2016-01-01

    Full Text Available This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment, which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum, serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P<0.05 the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P<0.05 the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P<0.05 when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P<0.05 the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  17. Effects of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854)

    Science.gov (United States)

    Zhu, Zhiming; Song, Bolan; Lin, Xiaotao; Xu, Zhongneng

    2016-01-01

    This study examines the effect of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb ( Barbonymus schwanenfeldii). Blood samples were taken on days 1, 23 and 45 from control fish and from two training groups maintained at current speeds of 0.06 bl/s (body length per second), 0.66 bl/s, and 1.92 bl/s, respectively. Significantly increased red-blood-cell counts and hematocrit were observed in the post-training groups on days 23 and 45. Significantly increased hemoglobin concentrations were also observed in the 1.92 bl/s group on days 23 and 45. In contrast, values of mean corpuscular volume were significantly lower in the 1.92 bl/s group than in the other groups on day 45. Nitroblue-tetrazolium-positive cells and lysozyme and superoxidase dismutase activities in the plasma increased significantly with increasing training intensity on days 23 and 45. Antibacterial activities were significantly increased in the trained groups compared with the control group on day 23; significantly elevated alkaline phosphatase activity was observed in the 1.92 bl/s groups on day 45. Therefore, training intensities of 0.66 and 1.92 bl/s enhanced the blood oxygen-carrying capability and plasma immune parameters of juvenile tinfoil barbs.

  18. Biological variation of platelet parameters determined by the Sysmex XN hematology analyzer.

    Science.gov (United States)

    Buoro, Sabrina; Seghezzi, Michela; Manenti, Barbara; Pacioni, Aurelio; Carobene, Anna; Ceriotti, Ferruccio; Ottomano, Cosimo; Lippi, Giuseppe

    2017-07-01

    This study was aimed to define the short- and medium-term biological variation (BV) estimates, the index of individuality and the reference change value (RCV) of platelet count, platelet distribution width, mean platelet volume, platelet larger cell ratio, plateletcrit and immature platelet fraction. The study population consisted of 43 health subjects, who participated to the assessment of medium-term (21 subjects; blood sampling once a week for 5 consecutive weeks) and short-term (22 subjects; blood sampling once a day for 5 consecutive days) BV study, using Sysmex XN-module. Eight subjects were also scheduled to participate to both phases. The data were subject to outlier analysis prior to CV-ANOVA, to determine the BV estimates with the relative confidence intervals. The medium-term and short-term within-subject BV (CV I ) was comprised between 2.3 and 7.0% and 1.1-8.6%, whereas the medium-term and short-term between-subjects BV (CV G ) was comprised between 7.1 and 20.7% and 6.8-48.6%. The index of individuality and index of heterogeneity were always respectively 0.63 for all the parameters, in both arms of the study. The RCVs were similar for all parameters, in both arms of the study. This study allowed to define the BV estimates of many platelet parameters, some of them unavailable in literature. The kinetics of platelet turnover suggests the use of short-term BV data for calculating analytical goals and RCV. The correct clinical interpretation of platelet parameters also necessitates that each laboratory estimates local RCV values. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Effect of natural zeolite on biochemical and hematological parameters in blood, body mass and growth of calves

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    Šamanc Horea

    2008-01-01

    Full Text Available The work presents the results of investigations of the effect of natural zeolite (clinoptilolite type on the degree of resumption of cloistral immunoglobulin, the value of hematological parameters and enzyme activity in blood serum (AST, ALT, and LDH, body mass, and daily growth of calves in the first three months of life. Investigations were carried out on three groups of 10 male and female calves each, with a 1:1 ratio of sexes, (control, K, and two experimental groups, I and II, of the black-and-white breed, Holstein type. All three groups of calves were fed with the usual rations (colostrum, collective cows' milk, fodder mix with 18% total proteins, and Lucerne hay.The experimental groups of calves, as opposed to the controls, received colostrums and collective cows' milk with the addition of a preparation based on natural zeolite in quantities of 5 and 10 g/l milk. The concentration of immunoglobulin G in blood serum of experimental groups of calves was significantly higher than the values of the control group (35.9±6.43 g/l in Group I; 32.5±7.39 g/l in Group II; 20.8±6.88 g/l in control group K. The results of the investigations of the values of hematological parameters (number of erythrocytes, hemoglobin concentration, and hematocrit value do not show any digressions from the physiological values and are approximately the same in the experimental and the control groups of calves, which indicates that the examined quality of the zeolite-based preparation did not affect the degree of the resumption of iron and other oligoelements significant for erythropoietin. The data on the enzyme activity in blood serum indicate that the examined preparation does not cause any significant functional or morphological changes in the tissue of parenchymatous organs or muscles. There were no significant disorders in the health of calves of the experimental groups, while 3 calves of the control group had gastrointestinal disorders (diarrhea. Calves of

  20. Hematological parameters and the variations resulting from stress of Alouatta caraya during a wildlife rescue program in Brazil.

    Science.gov (United States)

    Sánchez-Sarmiento, Angélica María; Zwarg, Ticiana; Fernandes-Santos, Renata Carolina; Guimarães-Luiz, Thaís; Genoy-Puerto, Alexander; Matushima, Eliana Reiko

    2015-03-01

    Deforestation and habitat fragmentation are major threats to the conservation status of New World primates, such as the howler monkey (Alouatta caraya) in Brazil, where vegetation destruction is often associated with projects such as Small Hydro Power Plant (SHP) construction. The resulting stress from the implementation of this type of enterprise may be a factor that influences individual susceptibility against pathogens and can determine the success or failure of mitigation measures proposed by responsible and/or requested companies by environmental agencies. To perform health monitoring and to understand physiological changes that ensued from the stress of capturing and keeping primates in captivity, we determined the blood profile of A. caraya rescued at the time of capture (N = 15) and in captivity (N = 11). Complete blood counts (CBCs) performed at the time of capture showed hematological changes compatible with acute stress, such as evident neutrophilic leukocytosis (WBCs; females = 20.48 ± 7.26; males = 18.78 ± 10.39 × 10(9) /L). In captivity, monocytosis was observed in both sexes (females = 1.34 ± 0.26; males = 0.39 ± 0.32 × 10(9) /L; U-test, P = 0.01), suggesting chronic stress. In females, there was also statistically significant eosinopenia (0.28 ± 0.10 × 10(9) /L; U-test, P = 0.01) and hematocrit increases (39.00 ± 1.41%; U-test, P = 0.01). Thus, data shows the impact of both capture related acute stress as well as captivity chronic stress, suggesting that primate management in the implementation of these projects causes significant changes in physiological parameters and, consequently, animal health. Whereas chronic stress is an inducer of immunosuppression and susceptibility to pathogen factors, monitoring hematological parameters in captive animals can act as an indicator of health status, contributing to the success of management and conservation wildlife programs. Am

  1. [Study on the screening program of thalassemia and the genotype and hematologic parameter among people of productive age in a village, Nanning Guangxi].

    Science.gov (United States)

    Zhang, Xin-Hua; Zhou, Ying-Jie; Li, Ping-Ping; Luo, Rui-Gui; Ruan, Li-Ming; Wang, Rong-Xin; Wu, Zhi-Kui; Li, Min; Huang, You-Wen

    2006-09-01

    To investigate the carrier ratio and the genotype of thalassemia in the rural people of reproductive age in Nanning, and to analyze the characteristics of hematologic parameter in thalassemia carriers. 2044 cases of productive age youths were detected with hemoglobin autoanalyse-Variant (HPLC) and Cell Dyn 1700 automatic hemocyte analysator. Among them,430 cases (75 couples randomly selected in thalassemia screening, 140 couples who were told that one or both of them were positive for thalassemia phenotype through hemocyte analysis) carried out thalassemia gene detection in synchronism. 163 cases were detected beta-thalassemia and the thus beta-thalassemia carrier ratio was 7.97%. 13 cases were detected HbH disease, and 2 cases Hb Manitoba, 2 cases HbJ, and 1 case HbQ. As for genotypes,-alpha (3.7)/alpha,-alpha(CS)/alphaalpha and -alpha(WS)/alphaalpha were common ones with in alpha-thalassemia-2, --(SEA)/alphaalpha the most common one in alpha-thalassemia-1, and 41-42 were the most common ones in beta-thalassemia heterozygotes. The detection ratio of alpha,beta combination thalassemia was also relatively high. Mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were low in all cases of HbH disease and beta-thalassemia, also low in 86 cases of alpha-thalassemia-1 with the exception of normal MCH in 1 case, yet normal in 17 cases out of 66 cases of alpha-thalassemia-2. HbF raised in 32 cases out of 69 cases of beta-thalassemia heterozygote, no case showed raised HbF without the raise of HbA2. Hematologic characteristic of alpha, beta combination thalassemia was mainly caused by beta-thalassemia. Carrier ratio of thalassemia in rural productive age youths in Nanning was high while alpha-thalassemia-2 with the genotype -alpha(WS)/alphaalpha and -alpha(CS)/ alphaalpha were common. To those with low MCV and MCH in high-risk region, thalassemia should be suspected.

  2. Noise elicits hematological stress parameters in Mediterranean damselfish (Chromis chromis, perciformes): A mesocosm study.

    Science.gov (United States)

    Vazzana, Mirella; Celi, Monica; Arizza, Vincenzo; Calandra, Giampiero; Buscaino, Giuseppa; Ferrantelli, Vincenzo; Bracciali, Claudia; Sarà, Gianluca

    2017-03-01

    In the last few decades, technological developments and the widespread rise of anthropic activities have increased the exposure of organisms to noise pollution, thus evoking great interest in its biological effects, particularly on the immune system. The aim of the present work was to investigate some of the biochemical parameters in the blood of Chromis chromis (Linnaeus, 1758) following in vivo exposure to noise levels of 200 and 300 Hz. Our results revealed that, compared to the control specimens, the fish exposed to noise had significantly increased levels of stress biomarkers such as glucose, lactate and total proteins in plasma, as well as a rise in the expression of heat shock protein 70 (HSP70). Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Normal Hematological, Biochemical, and Serum Electrolyte Values for a Colony of Rhesus Monkeys ’Macaca mulatta’,

    Science.gov (United States)

    1976-10-28

    provided by NAMRL Biomedical Sciences Division and Veterinary Sciences Division personnel are appreciated. The authors are especially grateful to Don...insufficiency in the rhesus monkey. Endocrinology , 50: 664-676, 1952. 7. Hartmen, C.G., and Straus, W.L., Jr. (Ed.), The An y. of the Rhesus Mok (caca...D.P., Valerjo, M.G., and -f Rininger, B.F., Hematologic changes associated with pregnancy and parturition in Macaca mulatta. Lab. Anim. Care, 20

  4. The Effect of Silvernano Oral Consumption on some Hormonal, Hematological and Urine Parameters of Vistar Rats

    Directory of Open Access Journals (Sweden)

    Roshanai K

    2012-09-01

    Full Text Available Background and Objectives: Silver nanoparticles with excellent antimicrobial effects and increasing consumption in different industries are the most widely used nanoparticles and so it is necessary to evaluate the safety of their use. This study has examined the effects of time and consumption dose of these particles on rats of Vistar race.Methods: 36 Vistar race rats after 4 weeks equalization period divided into 6 groups (one control and five tests and were received 95ppm, 65ppm, 35ppm, 20ppm, 5ppm concentrations of silver nano (Nanocid L2000 solution from NANONASB-E-PARS CO. as water. After 3 & 6 months 3 animals of each group were selected and hormonal parameters were determined by CLIA method, Hb and HCT was measured by common Med. Lab. Kits and blood cells counted by H1 equipment. Finally, results were processed by one-side variance and tukey's test.Results: The results indicated that white blood cells counting show meaningful dose dependent decreasing in number of these cells. No tangible change was seen on counting of red blood cells and the level of hemoglobin and hematocrit parameters. Also blood cortisol & T4 level of dependent dose decreased and Testosterone increased meaningfully. Water uptake index increasing happened in meaningful and dose dependent manner. Ketone bodies appeared in rats' urine that had eaten high doses of silver nano. Conclusion: WBC count decreasing after oral consumption of silver nano particles show cell apoptosis and immunity lowering. Also the particles have no effect on bone marrow function indexes. On the other hand, lowering the function of adrenal gland, hypothyroidism, increasing in sexual hormone level are side effects of these particles consumption. In addition, increasing in Water Uptake Index and urine volume can be for high blood pressure or kidney failure caused by these particles.

  5. Effects of High Levels of Deoxynivalenol and Zearalenone on Growth Performance, and Hematological and Immunological Parameters in Pigs

    Science.gov (United States)

    Reddy, Kondreddy Eswar; Song, Jaeyong; Lee, Hyun-Jeong; Kim, Minseok; Kim, Dong-Wook; Jung, Hyun Jung; Kim, Bumseok; Lee, Yookyung; Yu, Dongjo; Kim, Dong-Woon; Oh, Young Kyoon; Lee, Sung Dae

    2018-01-01

    Background: Deoxynivalenol (DON) and zearalenone (ZEN) are common food contaminants produced by Fusarium sp. Mycotoxins are a potential health hazard because of their toxicological effects on both humans and farmed animals. Methods: We analyzed three groups of pigs: a control group (fed a standard diet), and the DON and ZEN groups, fed a diet containing 8 mg/kg DON and 0.8 mg/kg ZEN respectively, for four weeks. Results: DON and ZEN exposure decreased body weight (BW), average daily feed intake (ADFI), food conversion rate (FCR), and the serum levels of immunoglobulin (Ig)G and IgM. The total antioxidant levels significantly decreased in serum and increased in urine samples of both treatment groups. Additionally, DON and ZEN exposure increased serotonin levels in urine. Hematological parameters were not affected by the investigated toxins. Microscopic lesions were evident in sections of kidneys from either treatment group: we found sporadic interstitial nephritis in the DON group and renal glomerulus atrophy in the ZEN group. The expression levels of inflammatory cytokines and chemokine marker genes were reduced in tissues from DON- and ZEN-exposed pigs. Conclusions: chronic ingestion of high doses of DON and ZEN alters the immune response and causes organs damage, and might be associated with various diseases in pigs. PMID:29518941

  6. Seroprevalence of hepatitis B surface antigenemia and its effects on hematological parameters in pregnant women in Osogbo, Nigeria.

    Science.gov (United States)

    Kolawole, Olatunji M; Wahab, Abideen A; Adekanle, Daniel A; Sibanda, Timothy; Okoh, Anthony I

    2012-12-27

    The transmission of the hepatitis B virus (HBV) is parenteral, sexual and perinatal. Prevention of vertical transmission of HBV is extremely important because HBV infection in early life usually results in a chronic carrier State. A descriptive seroepidemiological study of hepatitis B virus and its effects on hematological parameters was investigated in pregnant women attending antenatal clinic of LAUTECH Teaching Hospital, Osogbo, Nigeria. 200 venous samples were subjected to full blood count and its sera were subjected to enzyme-linked immunosorbent assay for the detection of surface antigen of hepatitis B virus. Prevalence rate of 16.5% was obtained for hepatitis B surface antigen in pregnant women. The highest HBsAg prevalence rate recorded was 23.3% for pregnant women between aged 30-34 years while the lowest recorded was zero percent for those aged greater than 40 years. RBC, WBC, neutrophil, hemoglobin lymphocyte and platelet counts have no significant effects on HBsAg positivity of pregnant women (p=0.801). There was no significant difference in HBsAg positivity in relation to maternal age, gravidity, gestational age, family type, level of education and occupation (p=0.073). Among the potential risk factors, there was significant difference in HBsAg positivity in the pregnant women in relation to their history of HBV vaccination (p=0.039). We advocate universal free screening of pregnant women as the endemicity of HBV infections is thus being propagated.

  7. PREVALENCE OF TRICHOMONIASIS IN DOMESTIC AND WILD PIGEONS AND ITS EFFECTS ON HEMATOLOGICAL PARAMETERS

    Directory of Open Access Journals (Sweden)

    M. H. SALEEM, M. S. KHAN, A. S. CHAUDRY AND H. A. SAMAD

    2008-06-01

    Full Text Available The present study was conducted to determine the prevalence of trichomoniasis and its effect on some blood parameters in pigeons. A total of 100 samples from the pigeons (50 wild and 50 domestic were collected during the months of March and April 2005. Higher prevalence (P<0.05 was recorded in wild pigeons (60% than in domestic pigeon (26%. The overall prevalence recorded was 43%, being non significantly higher in April (56% than in March (30%. In infected pigeons, there was significant (P<0.05 decrease in hemoglobin concentration number of monocytes packed cell volume, body weight, than healthy birds. Likewise, the values of total leukocyte count, lymphocytes and eosinophils were higher significantly (P<0.05 in infected pigeons than the healthy ones. While, no significant difference was observed for heterophils count when infected and healthy birds were compared. It was concluded that trichomonad infection is quite common in wild, as well as in domestic, pigeons under the prevailing cage system.

  8. Basic biochemical, hematological and hormonal parameters for monitoring the health and nutritional status in athletes

    Directory of Open Access Journals (Sweden)

    Aritz Urdampilleta

    2014-09-01

    Full Text Available Sporting competitions are becoming more demanding in terms of intensity of effort, and this means controlling all aspects that affect athletic performance. Food, hydration and supplementation, before, during and after training or competition directly affect health, body composition, performance and recovery of the athlete. The assessment of nutritional status is required for proper advising of the athlete, through blood tests to control the process of adaptation to training. The aim of this paper is to provide practical tools for dietitiansnutritionists to control the health and nutritional status of athletes, as well as monitoring their adaptation to workloads and competition periods. Performing analytical tests to control of protein metabolism, lipid profile, ions, blood tests and iron metabolism, in addition to review some hormonal parameters, may be of interest in order to observe the potential existence of overtraining states. The correct understanding and interpretation of laboratory tests (under sports doctor’s supervision will be most important and useful for dietitiansnutritionists, performing dietary and nutritional advice to athletes, because it will determine the status of the athlete and propose different individual feeding strategies depending on the training phase and response.

  9. Effect of Hippophae leaves on neurotransmitters and hematological parameters in whole body irradiated rats

    International Nuclear Information System (INIS)

    Gupta, Vanita; Prasad, Jagdish; Madhu Bala

    2012-01-01

    Till date no approved radio-protective agent is available world over. WR-2721 had severe side effects and was behaviourally toxic even at sub-lethal doses of ionizing radiation. Seabuckthorn (Hippophae rhamnoides L.) is known for its nutraceutical and therapeutic values. Our studies demonstrated that treatment with leaves of H. rhamnoides rendered > 90% whole body radioprotection in 60 Co-g-irradiated (10 Gy) mice population in comparison to 100% death in non-Hippophae treated irradiated (10 Gy) mice population. Our studies also demonstrated that treatment with leaves of H. rhamnoides prevented conditioned taste aversion (CTA) in irradiated (2 Gy) Sprague-Dawley rats. The present study was planned to evaluate the effects of aqueous extract of Hippophae leaves on changes in levels of neurotransmitters ((acetylcholine esterase (AChE) and dopamine (DA)) in plasma and brain, haematological parameters in blood/plasma; and brain histology in Sprague-Dawley rats showing CTA after 60 Co-g-irradiation (2 Gy). The results showed that whole body 60 Co-g-irradiation (2 Gy) (i) increased the levels of Ach, Eepinephrine (E) and norepinephrine (NE); oxidative stress (MDA and NO), and (ii) decreased the levels of DA; WBC counts and RBC counts and antioxidants (GSH), in comparison to untreated control. Treatment with 12 mg/kg b.w. drug concentration, prior to irradiation significantly (p<0.05) (i) decreased the levels of AChE, E and NE, and MDA and NO levels in plasma and brain, and (ii) increased the WBC counts; RBC counts and levels of antioxidants (GSH), in comparison to radiation control group. Histological changes in brain were also recorded. The results demonstrated that Hippophae leaves extract had neuro-protective and reduced oxidative stress in brain of whole body irradiated mice and could be, thereby contributing to behavioural protection. (author)

  10. Hematological and plasma biochemical parameters of aquarium-maintained cownose rays.

    Science.gov (United States)

    Ferreira, Claudia M; Field, Cara L; Tuttle, Allison D

    2010-06-01

    There is generally a dearth of information involving reference ranges of health variables for numerous elasmobranch species commonly housed in zoos and aquaria; thus, extrapolation from a few existing elasmobranch studies is commonly used to assess health parameters in these species. The primary objective of this study was to establish baseline complete blood cell count and plasma chemistry reference ranges for captive individuals of the cownose ray Rhinoptera bonasus, an elasmobranch that is widely displayed in zoos and aquaria worldwide. This study was conducted using 18 adult cownose rays currently housed at the Mystic Aquarium and Institute for Exploration in Connecticut. Median blood and plasma values were 550 cells/microL for total white blood cell count; 511,250 cells/microL for total red blood cell count; 31% for packed cell volume; 5.8 g/L for total solids; 2.85 g/dL for total protein; 33 units (U)/L for aspartate aminotransferase; 34 U/L for alkaline phosphatase; 0.2 mg/dL for total bilirubin; 1,155 mg/dL for urea nitrogen; 0.1 mg/dL for creatinine; 0.6 g/dL for albumin; 2.15 g/dL for globulins; 144 mg/dL for cholesterol; 157 mg/dL for triglycerides; 45 mg/dL for glucose; 16.9 mg/dL for calcium; 5.8 mg/dL for phosphorus; 294 mmol/L for sodium; 1.55 mmol/L for potassium; and 270 mmol/L for chloride. Gamma glutamyl transpeptidase, amylase, creatine kinase, and lactate dehydrogenase were below the detectable limits for several samples. In nine samples, alanine aminotransferase levels were below the instrument range (< 4 U/L). No significant differences between sexes were detected. The reference ranges reported in this study should provide a useful guide for routine health monitoring of captive cownose rays.

  11. Hematological Parameters and the State of Liver Cells of Rats After Oral Administration of Aflatoxin B1 Alone and Together with Nanodiamonds

    Science.gov (United States)

    Mogilnaya, O. A.; Puzyr, A. P.; Baron, A. V.; Bondar, V. S.

    2010-05-01

    Hematological parameters and the state of liver cells of rats were examined in vivo after the animals received aflatoxin B1 (AfB1) alone and together with modified nanodiamonds (MND) synthesized by detonation. The rats that had received the MND hydrosol had elevated leukocyte levels, mainly due to higher granulocyte counts and somewhat increased monocyte counts compared to control rats. Hematological parameters of the rats that had received AfB1 alone differed from those of the control rats in another way: total white blood cell counts were significantly lower due to the decreased lymphocyte counts. In rats that had consumed AfB1 with the MND hydrosol, changes in hematological parameters were less pronounced than in rats that had consumed either AfB1 or MND. Electron microscopy showed that hepatocytes of the rats that had received the MND hydrosol or AfB1 with the MND hydrosol contained elevated levels of lipid inclusions and lysosomes. Hyperplasia of the smooth endoplasmic reticulum (EPR) was revealed in liver specimens of the rats that had received AfB1. Results of the study suggest the conclusion about mutual mitigation of the effects of nanoparticles and the mycotoxin on rats blood and liver cells after AfB1 has adsorbed on MND.

  12. Hematological Parameters and the State of Liver Cells of Rats After Oral Administration of Aflatoxin B1 Alone and Together with Nanodiamonds

    Directory of Open Access Journals (Sweden)

    Baron AV

    2010-01-01

    Full Text Available Abstract Hematological parameters and the state of liver cells of rats were examined in vivo after the animals received aflatoxin B1 (AfB1 alone and together with modified nanodiamonds (MND synthesized by detonation. The rats that had received the MND hydrosol had elevated leukocyte levels, mainly due to higher granulocyte counts and somewhat increased monocyte counts compared to control rats. Hematological parameters of the rats that had received AfB1 alone differed from those of the control rats in another way: total white blood cell counts were significantly lower due to the decreased lymphocyte counts. In rats that had consumed AfB1 with the MND hydrosol, changes in hematological parameters were less pronounced than in rats that had consumed either AfB1 or MND. Electron microscopy showed that hepatocytes of the rats that had received the MND hydrosol or AfB1 with the MND hydrosol contained elevated levels of lipid inclusions and lysosomes. Hyperplasia of the smooth endoplasmic reticulum (EPR was revealed in liver specimens of the rats that had received AfB1. Results of the study suggest the conclusion about mutual mitigation of the effects of nanoparticles and the mycotoxin on rats blood and liver cells after AfB1 has adsorbed on MND.

  13. Hematologic emergencies

    Directory of Open Access Journals (Sweden)

    Daniele Vallisa

    2012-01-01

    Full Text Available In recent years, the surprising progress made in other areas of hematology (advances in the understanding of leukemogenesis, improved transplant techniques has been conspicuously absent in the management of hematologic emergencies. And yet, every step toward greater knowledge, every new treatment option will be of little value unless we are able to manage the acute complications of hematologic diseases. These complications are better defined as hematologic emergencies, and they are characterized by a high rate of mortality. This review is based on a search of the literature that was initially confined to articles published in the journal Hematology from 2000 to 2009. The search was then extended to the Cochrane Library and to Pub Med in February 2010 with the following Keywords emergencies; urgencies; hematology. The same key words were employed in a search of the archives of Blood and the New England Journal of Medicine from 2000 to 2010. The results confirm that hematologic emergencies can be caused by hematologic malignancies as well as by non-neoplastic hematologic diseases. Within the former category; this review examines the causes; manifestations; treatment and prevention of disseminated intravascular coagulation; superior vena caval syndrome; spinal cord compression; tumor lysis syndrome; hyperleukocytosis; and hypercalcemia. We also review emergency situations associated with non-neoplatic haematological diseases; such as thrombotic thrombocytopenic purpura; drug-induced hemolytic anemia; and acute sickle-cell crisis.

  14. Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis

    OpenAIRE

    Alekish, Myassar O.; Ismail, Zuhair B.; Awawdeh, Mofleh S.; Shatnawi, Shoroq

    2017-01-01

    Aim: The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. Materials and Methods: The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of s...

  15. Ameliorative properties of ethyl acetate fraction of Ceiba pentandra on serum glucose, hematological and biochemical parameters of diabetic rats

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    Muhammad Hadiza Lami

    2015-09-01

    Full Text Available Objective: To evaluate the antidiabetic potential of Ceiba pentandra leaves used by some Nupe speaking community of Niger State, Nigeria in folkloric management of diabetes. Methods: Fifteen albino rats of both sexes weighing between 100 and 160 g were randomly allotted to five groups of four rats each. Alloxan monohydrate (110 mg/kg body weight was intraperitoneally administered to rats in their respective groups, and rats with blood glucose (200 mg/kg body weight were considered diabetic. Diabetic rats in their respective groups received 2.5 mg/kg body weight of standard drug (glibenclamide, 200 and 400 mg/kg body weight of extract once daily for 12 days. Normoglycemic group (reference group I received 0.5 mL normal saline, while the last group was untreated diabetic (reference group II. The blood glucose was measured by using Accu-Chek Active glucometer every three days and the experiment was terminated at 17th day. Results: Blood glucose decreased significantly (P < 0.05 in all the treated groups during the period of treatment with highest hypoglycaemic activity observed in 200 mg/kg body weight group. The diabetic untreated group showed significant reduction (P < 0.05 in body weight as it was a clinical feature of diabetes in reference to normoglycemic, and other treatment groups. Platelets showed a significant decrease and increase respectively in the untreated and treated groups in reference to the normoglycemic group. Decreased packed cell volume, red blood cells and hemoglobin count, and an increase in white blood cell were also observed in the untreated group. Body weight of the treated groups remained stable as against the reference group II. Activities of the serum alanine aminotransferase, aspartate aminotransferase, chloride and potassium increased significantly (P < 0.05 in standard drug while carbonate and sodium showed the opposite. The urea, creatinine, total and conjugated bilirubin all increased significantly (P < 0.05 in the

  16. Changes of hematological and biochemical parameters revealed genotoxicity and immunotoxicity of neonicotinoids on Chinese rare minnows (Gobiocypris rarus).

    Science.gov (United States)

    Hong, Xiangsheng; Zhao, Xu; Tian, Xue; Li, Jiasu; Zha, Jinmiao

    2018-02-01

    Adverse impacts of immunity in terrestrial non-target organisms exposed to neonicotinoid insecticides have been reported, but the causal link between insecticide exposure and possible immune alterations in fish remains limited. In the present study, the potential genotoxicity and immunotoxicity of three neonicotinoids (imidacloprid, nitenpyram, and dinotefuran) were assessed in Chinese rare minnows by using a 60-day chronic toxicity test. The hematological and biochemical parameters of juvenile Chinese rare minnows and changes in the transcription of six inflammation-related genes were determined after exposure to neonicotinoids at 0.1, 0.5, or 2.0 mg/L. A clear difference in the frequency of erythrocytes with micronuclei (MN) was observed after treatment with 2.0 mg/L imidacloprid (p < .05). Additionally, exposure to 0.5 or 2.0 mg/L imidacloprid significantly increased the binucleated (BN) erythrocytes and those with notched nuclei (NT) (p < .05). A serum protein electrophoresis (SPE) assay showed significant alterations in the serum protein in all treatments (p < .05), and further analysis indicated decreases in immunoglobulin (Ig) in treatments with 0.5 or 2.0 mg/L imidacloprid or dinotefuran or with 0.1 mg/L nitenpyram (p < .05). Moreover, a biochemical assay confirmed that immunoglobulin M (IgM) levels were indeed significantly decreased upon treatment with imidacloprid or dinotefuran at 0.5 or 2.0 mg/L (p < .05). In addition, the transcriptional levels of the inflammatory cytokines IL-6, INF-α, TNF-α, and IL-1β were markedly down-regulated after all imidacloprid treatments (p < .05), whereas the expression levels of only TNF-α and IL-1β were significantly down-regulated following the 0.5 and 2.0 mg/L dinotefuran treatments (p < .05). Taken together, our results clearly demonstrate that imidacloprid, rather than nitenpyram and dinotefuran, can induce genotoxicity. The responsiveness of these immune indicators

  17. Changes of Selected Hematological Parameters and Morning Rest Rate during Ten Days High Altitude Stay and Training

    Directory of Open Access Journals (Sweden)

    Jiří Suchý

    2015-03-01

    Full Text Available Changes of Selected Hematological Parameters and Morning Rest Rate during Ten Days High Altitude Stay and Training This article describes the influence of a ten day stay and training in a high altitude area (1850 m ASL on selected blood count parameters and morning resting heart rate. Three tests were performed on a group of young cross country skiers (n = 10, age: 18.7 ± 4.8: two days before, two days after and ten days after the altitude training camp. Two day after the return, significant (p 0.05. The study in a group of young cross country skiers has shown positive effects of a tenday training camp in high altitude on blood count parameters and morning resting heart rate monitored in lowland two and ten days after the return from high altitude. Změny vybraných hematologických parametrů a ranní klidové srdeční frekvence v průběhu desetidenního tréninku a pobytu ve vyšší nadmořské výšce Článek popisuje vliv desetidenního tréninku a pobytu ve vyšší nadmořské výšce (1850 m n. m. na vybrané parametry krevního obrazu a ranní klidové srdeční frekvence. U skupiny mladých běžců na lyžích (n = 10, věk: 18,7 ± 4,8 byly realizovány celkem tři odběry krve: dva dny před soustředěním ve výšce, dva dny a deset dnů po návratu do nížiny. Dva dny po návratu byly signifikantně (p 0,05. Průměrné hodnoty ranní klidové srdeční frekvence byly během soustředění ve výšce vyšší než před odjezdem v nížině (p 0,05. Námi realizovaný výzkum u skupiny mladých běžců na lyžích prokázal pozitivní efekt desetidenního pobytu a tréninku ve vyšší nadmořské výšce (1850 m n. m. na sledované krevní parametry a ranní klidovou srdeční frekvenci v nížině po návratu.

  18. Effect of Caffeine and trans-cinnamaldehyde on Growth Performance, Hematology, Stress Hormone, Immunity Response and Blood Parameters in Broiler Chickens

    Directory of Open Access Journals (Sweden)

    Pournia KH

    2015-06-01

    Full Text Available This experiment was conducted to evaluate the effects of caffeine and trans-cinnamaldehyde (TC on growth performance, hematological parameters, stress hormones, immune response, and some blood parameters of broiler chickens. Three hundred fifty 1-d-old broiler chicks were randomly assigned to 7 dietary treatments (five pens/ treatment of 10 male broilers each in a completely randomized design. A basal control corn-soybean meal diet for starter, grower and finisher periods were formulated and supplemented with appropriate levels of caffeine (0.5, 1 and 2% or TC (0.5, 1 and 1.5%. Findings showed that caffeine at level of 2% decreased feed intake (FI, body weight (BW, body weight gain (BWG and increased feed conversion ratio (FCR. TC supplementation had no significant effect on growth performance compare to control diet. Except caffeine which significantly decreased hemoglobin and hematocrit at level of 2% in 21 day old chicks (P, caffeine and TC had no significant effects on hematological parameters and stress hormone of  broiler chickens in 21 and 42 d (P>0.05. Similar trend were observed for humeral and cell mediated immunity. Supplementation of 2% of caffeine significantly decreased glucose level in 21 d (P and this effect was not observed for TC levels compare with the control treatment. On the other hand all levels of caffeine and TC at level of 1% significantly decreased cholesterol levels in 21 d compared to control treatment (P. Caffeine and TC levels were not change the blood parameters in 42 d. Findings showed that supplementation of diets with caffeine specially at level of 2% decrease growth performance and neither caffeine nor TC affect the hematological, hormonal and immunity response in broiler chickens.

  19. Comparisons of stomatal parameters between normal and abnormal ...

    African Journals Online (AJOL)

    ED), guard cell length (GCL) and guard cell width (GCW) of normal and abnormal leaf of Bougainvillea spectabilis Willd were studied. This can be useful for further research of physical mechanism of abnormal leaf. Epidermal cells were ...

  20. The normative study of acoustic parameters in normal Egyptian ...

    African Journals Online (AJOL)

    , Alexandria Main University Hospital, on one hundred normal children of both sexes aged from 4 to 12 years, all children were subjected to computerized acoustic analysis using Multidimensional voice program software. The vocal samples ...

  1. Detection of innocent systolic murmurs by auscultation and their relation to hematologic and echocardiographic findings in clinically normal Whippets.

    Science.gov (United States)

    Bavegems, Valérie C; Duchateau, Luc; Polis, Ingeborgh E; Van Ham, Luc M; De Rick, André F; Sys, Stanislas U

    2011-02-15

    To determine murmur prevalence by auscultation of 105 apparently healthy Whippets without signs of cardiac disease, to determine the origin of these murmurs, and to evaluate the influence of sex, type of pedigree (ie, bred for showing or racing), and training on these murmurs. Cross-sectional study. 105 client-owned Whippets. All dogs were auscultated by the first author and underwent a complete physical and cardiological examination, together with a hematologic assessment. Several RBC variables and echocardiographic variables were compared between dogs with or without a murmur at the level of the aortic valve. 44 of 105 (41.9%) dogs had no murmur. A soft systolic murmur was present with point of maximal intensity at the level of the aortic valve in 50 (47.6%) dogs, at the level of the pulmonic valve in 8 (7.6%) dogs, and at the level of the mitral valve in 3 (2.9%) dogs. No significant differences were found in heart rate, rhythm, murmur presence, point of maximal intensity, and murmur grade between males and females, between dogs with race- and show-type pedigrees, or between dogs in training and not in training. Dogs with a murmur at the level of the aortic valve had a significantly higher aortic and pulmonic blood flow velocity and cardiac output, compared with dogs without a murmur. Whippets have a high prevalence of soft systolic murmurs in the absence of any structural abnormalities, which fit the description of innocent murmurs. No influence of sex, pedigree type, or training was found on the occurrence of these murmurs in Whippets.

  2. Iron, Hematological Parameters and Blood Plasma Lipid Profile in Vitamin D Supplemented and Non-Supplemented Young Soccer Players Subjected to High-Intensity Interval Training.

    Science.gov (United States)

    Jastrzebska, Maria; Kaczmarczyk, Mariusz; Suárez, Arturo Diaz; Sánchez, Guillermo Felipe López; Jastrzebska, Joanna; Radziminski, Lukasz; Jastrzebski, Zbigniew

    2017-01-01

    Vitamin D deficiency has been associated with increased risk for cardiovascular disease and anemia. Vitamin D-related changes in lipid profile have been studied extensively but the relationship between vitamin D and lipid metabolism is not completely understood. As both vitamin D and intermittent training may potentially affect iron and lipid metabolism, the aim of the study was to evaluate whether a daily supplementation of vitamin D can modulate the response of hematological and lipid parameters to high-intensity interval training (HIIT) in soccer players. Thirty-six young elite junior soccer players were included in the placebo-controlled, double-blind study. Participants were non-randomly allocated into either a supplemented group (SG, n=20, HIIT and 5,000 IU of vitamin D daily) or placebo group (PG, n=16, HIIT and sunflower oil). Hematological parameters were ascertained before and after the 8-wk training. The change score (post- and pre-training difference) was calculated for each individual and the mean change score (MCS) was compared between SG and PG using the t test and analysis of covariance. There were no differences between SG and PG at baseline. The red and white cell count, hemoglobin, hematocrit, MCHC, ferritin, and HDL-cholesterol changed significantly over the 8-wk HIIT. However, no significant differences in MCS were observed between SG and PG for any variable. A daily vitamin D supplement did not have any impact on alteration in hematological or lipid parameters in young soccer players in the course of high-intensity interval training.

  3. Effects of starvation and refeeding on the hematological and serum parameters and body proximate composition of Caspian salmon (Salmo trutta caspius) fingerligs

    OpenAIRE

    Zaefarian, A.; Yeganeh, S.; Oraji, H.; Jani khalili, Kh.

    2016-01-01

    This study was conducted to investigate the effects of periods of starvation and refeeding on some hematological, serum parameters and whole body proximate composition in Caspian salmon (Salmo trutta caspius). 240 samples of the Caspian salmon with an average weight of 13.73 ± 0.63 g were kept in 300 liter polyethylene tanks. Samples were fed twice a day with Rainbow trout commercial feed to the point of satiation. This experiment lasted for 10 weeks with control (without starvation) and 3 st...

  4. Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum).

    Science.gov (United States)

    Adel, Milad; Abedian Amiri, Armin; Zorriehzahra, Jalil; Nematolahi, Amin; Esteban, Maria Ángeles

    2015-08-01

    Peppermint (Mentha piperita L.) is a very popular herb. While numerous effects have been described in mammals, its effects on fish have received so far limited attention. The effects of dietary administration of peppermint on fry Caspian white fish (Rutilus frisii kutum) were studied. Fish were divided into 4 groups before being fed diets supplemented with 0% (control), 1%, 2% and 3% of peppermint extracts for 8 weeks. Dose-dependent increases of growth parameters (WG and SGR), mucus skin (protein concentration, alkaline phosphatase and antimicrobial activity) and seric (lysozyme and IgM) and blood leucocyte respiratory burst activities and different hematological parameters (number of red and white cells, seric hemoglobin and hematocrit content) were recorded in fry fish fed supplemented diets. However, the dietary peppermint supplements have different effects on the number of blood leucocytes depending on the leucocyte cell type. While no significant differences were observed in the number of blood monocytes and eosinophils, the number of neutrophils and lymphocytes was increased and decreased, respectively, on fish fed peppermint enriched diets, respect to the values found in control fish. Present results corroborate that dietary administration of peppermint promotes growth performance and increases the main hematological and immune humoral (both mucosal and systemic) parameters of fry Caspian white fish. This study may provide new applications of peppermint and, at the same time, promote rational development and utilization of peppermint resources. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. REPTILE HEMATOLOGY

    Directory of Open Access Journals (Sweden)

    Nejra Hadžimusić

    2013-03-01

    Full Text Available Determination of the number of circulating blood cells is of a great importance in clinical diagnosis. However, in some species, such as birds and reptiles, it is not possible to determine the number of individual blood cells using standard automated equipment, because of the specific morphological characteristics. For this reason, recognition of individual cell elements is crucial during hematological examination. Key words: Hematology, reptiles, blood cell morphology

  6. Normalized Dynamic Blood Pressure Parameters - Additional Marker of Hypertension Risk

    Czech Academy of Sciences Publication Activity Database

    Jurák, Pavel; Halámek, Josef; Vondra, Vlastimil; Leinveber, P.; Fráňa, P.; Plachý, M.; Souček, M.; Kára, T.

    2008-01-01

    Roč. 6, č. 1 (2008), s. 103 ISSN 1556-7451. [World Congress on Heart Disease /14./. 26.07.2008-29.07.2008, Toronto] Institutional research plan: CEZ:AV0Z20650511 Keywords : hypertension * vessel compliance * blood pressure * dynamic parameters Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  7. The normative study of acoustic parameters in normal Egyptian ...

    African Journals Online (AJOL)

    Yehia A. Abo-Ras

    2013-03-21

    Mar 21, 2013 ... Voice: the book tip. Rio de Janeiro: Revinter;. 2001. p. 85–245 (Article in Portugese). 14. Tavares E, Badra R, Martins R. Normative study of vocal acoustic parameters from children from 4 to 12years of age without vocal symptoms. A pilot study. Brazilian Journal of. Otorhinolaryngology 2010;76(4):485–90.

  8. Evaluation of behavioral parameters, hematological markers, liver and kidney functions in rodents exposed to Deepwater Horizon crude oil and Corexit.

    Science.gov (United States)

    Ramesh, Sindhu; Bhattacharya, Dwipayan; Majrashi, Mohammed; Morgan, Marlee; Prabhakar Clement, T; Dhanasekaran, Muralikrishnan

    2018-02-20

    The 2010 Deepwater Horizon (DWH) oil spill is the largest marine oil spill in US history. In the aftermath of the spill, the response efforts used a chemical dispersant, Corexit, to disperse the oil spill. The health impacts of crude oil and Corexit mixture to humans, mammals, fishes, and birds are mostly unknown. The purpose of this study is to investigate the in vivo effects of DWH oil, Corexit, and oil-Corexit mixture on the general behavior, hematological markers, and liver and kidney functions of rodents. C57 Bl6 mice were treated with DWH oil (80 mg/kg) and/or Corexit (95 mg/kg), and several hematological markers, lipid profile, liver and kidney functions were monitored. The results show that both DWH oil and Corexit altered the white blood cells and platelet counts. Moreover, they also impacted the lipid profile and induced toxic effects on the liver and kidney functions. The impacts were more pronounced when the mice were treated with a mixture of DWH-oil and Corexit. This study provides preliminary data to elucidate the potential toxicological effects of DWH oil, Corexit, and their mixtures on mammalian health. Residues from the DWH spill continue to remain trapped along various Gulf Coast beaches and therefore further studies are needed to fully understand their long-term impacts on coastal ecosystems. Copyright © 2017. Published by Elsevier Inc.

  9. Roles of Dietary Cobalt and Administration of Mixed Rumen Bacteria in Regulating Hematological Parameters of Pre-weaning Twin Lambs

    Directory of Open Access Journals (Sweden)

    T. Adelina

    2013-08-01

    Full Text Available Cobalt (Co is required by rumen microorganism for vitamin B12 synthesis. Vitamin B12 is an important cofactor for methionine synthesis and gluconeogenesis. In young ruminants up to 6–8 wk old, the rumen has not been completely developed and rumen microorganisms are not ready to supply vitamin B12. The aim of this research was to determine the potency of mixed rumen bacteria and dietary supplementation of Co and its effect on plasma glucose, blood minerals (Co, Fe, and Zn concentrations, and hematology of pre-weaning twin lambs. Twelve one month-old local twin lambs were assigned to 4 groups in a randomized complete block design. Lambs were fed cow milk at 10% body weight, adjusted weekly for 80 d. Mixed rumen bacteria were offered at 15 mL/d (8.295x1010 cfu. Dietary treatments were: 1 basal diet (Control, 2 basal diet + 1 mg/kg DM cyanocobalamin (VitB12 and 3 basal diet + 1 mg/kg DM of Co + administration of 15 mL mixed rumen bacteria (CoBac. There were no treatment effects on neither plasma glucose and blood mineral concentrations nor hematological profiles. This study demonstrated that pre-weaning twin lambs are not responsive to supplementation of Co and administration of mixed rumen bacteria.

  10. Effect of combination therapy of hydroxyurea with l-carnitine and magnesium chloride on hematologic parameters and cardiac function of patients with beta-thalassemia intermedia.

    Science.gov (United States)

    Karimi, Mehran; Mohammadi, Faranak; Behmanesh, Farzane; Samani, Soleiman M; Borzouee, Mohammad; Amoozgar, Hamid; Haghpanah, Sezaneh

    2010-01-01

    l-Carnitine and magnesium have antioxidant properties. They have the potential to stimulate production of fetal hemoglobin and stabilize the RBC membrane, respectively. Several studies have also shown the beneficial effects of hydroxyurea in thalassemic patients. We assessed the effect of combination therapy of hydroxyurea with l-carnitine and magnesium chloride on hematologic parameters and cardiac function of patients with beta-thalassemia intermedia. One-hundred-and-twenty patients with thalassemia intermedia (range, 4-35 yr; mean, 19 +/- 6.4 yr) who had no need for blood transfusion or requirement for blood transfusion with an interval of >6 months were randomly selected. All patients had been on hydroxyurea for >6 months. They were randomly divided into four groups: group A (hydroxyurea alone); group B (hydroxyurea and l-carnitine); group C (hydroxyurea and magnesium chloride); and group D (hydroxyurea, l-carnitine and magnesium chloride). In groups B, C, and D, mean Hb and hematocrit increased during 6-month treatment (P l-carnitine or magnesium could be more effective in improving hematologic parameters and cardiac status in patients with beta-thalassemia intermedia than hydroxyurea alone.

  11. Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil.

    Science.gov (United States)

    Fortes Carvalho Neta, Raimunda Nonata; Pinheiro Sousa, Débora Batista; de Macêdo Sobrinho, Inaldo Carvalho; Yarbrough Horton, Emily; da Silva de Almeida, Zafira; Tchaicka, Lígia; de Sousa, Alana Lislea

    2015-10-01

    Genotoxic and hematological parameters in tambaqui (Colossoma macropomum), a native freshwater fish, were used as biomarkers to assess exposure to environmental stressors within the Maracanã Protected Area of Maranhão State, Brazil. Fish were sampled at two sites-Serena Lagoon (control) and Ambude River-on four occasions (dry and rainy season), and biometric data (length and weight) recorded and blood collected from all fish for analysis. Erythrocyte indices-mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration-were calculated. Blood samples were stained with Giemsa and acridine orange, and microscopically examined for micronuclei and morphological nuclear abnormalities. Micronuclei were observed in fish from both sites, although the frequency was significantly higher in fish from the Ambude River and morphological nuclear abnormalities were only observed in fish from the Ambude River. More morphological nuclear abnormalities and a larger number of micronuclei were observed in erythrocytes stained with acridine orange compared with those stained with Giemsa. On average, erythrocyte indices were lower in fish from the Ambude River than from the Serena Lagoon. The results confirm that genotoxic and hematological parameters in C. macropomum can be used as indicators of environmental health and could be valuable tools for monitoring environmental conditions within protected areas.

  12. hemaClass.org: Online One-By-One Microarray Normalization and Classification of Hematological Cancers for Precision Medicine.

    Directory of Open Access Journals (Sweden)

    Steffen Falgreen

    Full Text Available Dozens of omics based cancer classification systems have been introduced with prognostic, diagnostic, and predictive capabilities. However, they often employ complex algorithms and are only applicable on whole cohorts of patients, making them difficult to apply in a personalized clinical setting.This prompted us to create hemaClass.org, an online web application providing an easy interface to one-by-one RMA normalization of microarrays and subsequent risk classifications of diffuse large B-cell lymphoma (DLBCL into cell-of-origin and chemotherapeutic sensitivity classes. Classification results for one-by-one array pre-processing with and without a laboratory specific RMA reference dataset were compared to cohort based classifiers in 4 publicly available datasets. Classifications showed high agreement between one-by-one and whole cohort pre-processsed data when a laboratory specific reference set was supplied. The website is essentially the R-package hemaClass accompanied by a Shiny web application. The well-documented package can be used to run the website locally or to use the developed methods programmatically.The website and R-package is relevant for biological and clinical lymphoma researchers using affymetrix U-133 Plus 2 arrays, as it provides reliable and swift methods for calculation of disease subclasses. The proposed one-by-one pre-processing method is relevant for all researchers using microarrays.

  13. Determination of selenium toxicity to Oreochromis niloticus based on hematological parameters - doi: 10.4025/actascibiolsci.v34i2.8755

    Directory of Open Access Journals (Sweden)

    Julio Vicente Lombardi

    2012-03-01

    Full Text Available Selenium (Se is described as an essential micronutrient and participates in different biological functions, as the antioxidant defense systems maintenance and regulation. However, when in high concentrations, Se may cause toxic effects as well as hematological changes in fish. The aim of the present study was to determine the toxicity of selenium in the form of sodium selenate (Na2Se6+O4 in Oreochromis niloticus based on hematological parameters, after exposure to different concentrations (0.01, 0.14 and 1.4 mg Se6+ L-1. The erythrocytic and leukocytic series were examined over 14 days at intervals of 0, 3, 5, 7,10 and 14 days. The erythrocytic series showed significant alterations in the first 7 days, including the control group. Neutrophils and monocytes showed variations in the first 3 days at a concentration of 1.40 mgSe6+ L-1 characterizing an acute response. The total number of leukocytes was different in relation to time zero on all Se concentrations. The thrombocyte count also differed statistically from time zero and control in the first 3 days at 0.14 mgSe6+ L-1. These results indicate that different concentrations induce an acute response with diminution of total leukocytes, neutrophilia, monocytosis and thrombocytosis. 

  14. Influência do parasitismo por nematódeos sobre o perfil hematológico de caprinos Influence of nematodes on the hematological parameters of goats

    Directory of Open Access Journals (Sweden)

    M.J.T. Mattos

    2005-02-01

    Full Text Available The interaction between ivermectin-resistant and ivermectin-sensitive nematodes and the effect of this anthelmintic on the hematological status of naturally infected goats was assessed using 36 animals. Of these animals, 12 were infected by ivermectin-sensitive gastrointestinal nematodes of the superfamily Trichostrongyloidea (G1s e G1s 12 were infected by ivermectin-resistant nematodes (G2r e G2r and 12 uninfect goats (G3np. Six infected goats of each group (G1sm e G2rm received oral ivermectin al the dose of 200µg/kg, while six were used as controls (G1sc e G2rc. Blood and fecal samples were collected on the day of medication (day zero, at seven, and at 14 days thereafter. The erythrocyte count and hematocrit levels in goats infected by sensitive strains of Haemonchus was greater than that of the group infected by a resistant strain. These values are lower in comparison to those observed in uninfected goats. The increase in the number of eggs per gram of feces, regardless of the strain, is inversely related to the hematocrit level. The use of ivermectin did not significantly change the hematological parameters of goats.

  15. Action of Brazilian propolis on hematological and serum biochemical parameters of Blue-fronted Amazons (Amazona aestiva, Linnaeus, 1758) in captivity.

    Science.gov (United States)

    Silva, Cínthia R B; Putarov, Thaila C; Fruhvald, Erika; Destro, Flavia C; Marques Filho, Wolff C; Thomazini, Camila M; Barbosa, Tatiana S; Orsi, Ricardo O; Siqueira, Edson R

    2014-07-01

    The present study aimed to evaluate the effect of propolis use on hematological and serum biochemical parameters in Blue-fronted Amazons (Amazona aestiva). For this, 12 adult birds were distributed randomly into individual cages, divided into treatments with different propolis levels (A = 0.0%; B = 0.5%; and C = 1.0%), in 3 distinct phases (I, II, and III), with 15-d duration for phases I and III and 30 d for phase II, totaling 60 d. In phases I and III, all birds received treatment A ration, and in phase II received A, B, or C (4 birds per treatment). At the end of each phase, blood was collected for biochemical and hematological evaluations. The variables were analyzed by ANOVA (P < 0.05). Results suggest that 0.5% propolis reduced lactate dehydrogenase levels, whereas treatment B augmented hemoglobin concentrations and eosinophil count. It is concluded that 0.5% propolis improves levels of lactate dehydrogenase, hemoglobin, and eosinophils. © 2014 Poultry Science Association Inc.

  16. Effects of Probiotic Bacteria Bacillus on Growth Performance, Digestive Enzyme Activity, and Hematological Parameters of Asian Sea Bass, Lates calcarifer (Bloch).

    Science.gov (United States)

    Adorian, Taida Juliana; Jamali, Hadi; Farsani, Hamed Ghafari; Darvishi, Paria; Hasanpour, Soleiman; Bagheri, Tahereh; Roozbehfar, Reza

    2018-02-09

    This study was conducted to evaluate different doses of two species of Bacillus (Bacillus licheniformis and Bacillus subtilis), on growth parameters, chemical composition of fish, activity of liver, and digestive enzymes of Asian sea bass. During 8 weeks, juvenile Asian sea bass received diets supplemented with 1 × 10 3 , 1 × 10 6 , and 1 × 10 9  CFU g -1 probiotic in addition to a control diet without added microorganisms. At the end of the trial, growth indices (total weight, total length, specific growth rate, total weight gain, food conversion ratio, and condition factor), body composition (crude protein, crude lipid, ash, and dry matter), digestive enzymes (protease, lipase, and amylase), liver enzymes [aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP)], immunologic indicators (lysozyme), and hematological parameters [hematocrit (Hct), hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs)] were assessed. Asian sea bass receiving diets supplemented with probiotic Bacillus (Bacillus licheniformis and Bacillus subtilis) showed significantly better growth than those fed the basal diet (control). Regarding body composition, total protein levels and dry matter were higher and lipid levels were lower in fish fed the diet containing 1 × 10 6  CFU g -1 probiotic compared with the control group (P Digestive enzymes (protease, lipase, and amylase) and hematological parameters (RBC, WBC, and Hb) were all highest in fish fed diet supplemented with 1 × 10 6  CFU g -1 probiotic Bacillus. Also, liver enzymes (AST, ALT, ALP) were lower in fish fed diet supplemented with 1 × 10 6  CFU g -1 probiotic Bacillus. Being that supplementation of 1 × 10 6  CFU g -1 of Bacillus in the diet is the dose which delivers the best results.

  17. Effects of Discontinuous Administration of Dietary Mannan oligosaccharide and β-1,3-glucan on Hematological and Blood Serum Biochemical Parameters of Rainbow trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    Reza Akrami

    2016-04-01

    Full Text Available Introduction Bans and restrictions of antibiotics as feed additives in fish culture in many countries have resulted in the increase in studies on alternative dietary supplements such as prebiotics to enhance the health and production of cultured fish. Prebiotics are nondigestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon. In addition, prebiotics promote the growth of lactic acid bacteria that are beneficial to health and lessen the density of pathogenic microorganisms. Because of the conservation of immune system, it is suggested that alternate administration of different immunostimulants may activate different parts of immune system of shrimps and take advantage of different immunostimulants to solve the problem of immunity fatigue and enhance the immunity continuously. However, there is no report on the discontinuous administration of immunostimulants for rainbow trout. Thus, considering the strategic importance of this species, the goal of this study was to assess the effects of discontinuous administration of dietary mannan oligosaccharide and β-1,3-glucan on hematological and blood serum biochemical parameters of rainbow trout. Materials and Methods A 6-week trial was conducted to compare the effects of discontinuous administration of dietary mannan oligosaccharide and β-1,3-glucan on hematological and blood serum biochemical parameters of rainbow trout (Oncorhynchus mykiss. Four feeding strategies were set, including feeding prebiotic-free diet continuously (control, feeding dietary prebiotic continuously (1.5 g kg-1, feeding dietary alternately (one week prebiotic+one week control diet and 2 Day prebiotic+5 Day control diet. Juveniles were fed the experimental diet in rate of 4% of the body weight per day, spread across two feeding time. At the end of the experiment, three fish were sampled randomly from each tank and were

  18. Radiation hematology

    International Nuclear Information System (INIS)

    Zherbin, E.A.; Chukhlovin, A.B.

    1989-01-01

    State-of-the-Art ofl radiation hematology and review of the problems now facing this brauch of radiobiology and nuclear medicine are presented. Distortion of division and maturation of hemopoiesis parent cells is considered as main factor of radiopathology for hematopoetic system. Problems of radiation injury and functional variation of hematopoetic microenvironment cell populations are discussed. 176 figs.; 23 figs.; 18 tabs

  19. Hematologic malignancies

    International Nuclear Information System (INIS)

    Hoogstraten, B.

    1986-01-01

    The principle aim of this book is to give practical guidelines to the modern treatment of the six important hematologic malignancies. Topics considered include the treatment of the chronic leukemias; acute leukemia in adults; the myeloproliferative disorders: polycythemia vera, essential thrombocythemia, and idiopathic myelofibrosis/agnogenic myeloid metaplasia; Hodgkin's Disease; non-Hodgkin's lymphoma; and Multiple Myeloma

  20. Sex-Related Differences in Hematological Parameters and Organosomatic Indices of Oreochromis niloticus Exposed to Aflatoxin B1 Diet

    Directory of Open Access Journals (Sweden)

    Esther Marijani

    2017-01-01

    Full Text Available A 24-week feeding experiment was conducted to assess whether males and females of Oreochromis niloticus exhibit differences in their hematological responses and organosomatic indices to dietary AFB1 contamination. Triplicate groups of O. niloticus (initial body weight: 24.1 ± 0.6 g were fed with four diets (Diets 1 to 4 containing 0, 20, 200, and 2,000 μg AFB1 kg−1. A significant decrease (P<0.05 in hemoglobin (Hb, red blood cells (RBC, and hematocrit (Hct was observed in AFB1 exposure groups, with the lowest levels recorded in the 2000 μg AFB1 kg−1 treatment. A significant increase in mean white blood cells (WBC, neutrophils, and lymphocytes was observed in AFB1 exposure groups. No sex-related differences in RBC, WBC, lymphocytes, monocytes, and neutrophils levels were observed. However, hemoglobin and hematocrit values for female O. niloticus were significantly lower than those for male O. niloticus. Organosomatic indices showed that the relative liver, kidney, and spleen weights were significantly higher (P<0.05 in the AFB1 supplemented group than in the control group. However, the effect of aflatoxin on organosomatic indices does not depend on sex but rather depends on the dose of aflatoxin in the diet. These results provide useful information for monitoring changes in the health status of male and female O. niloticus.

  1. The Effect on Some Hematologic Blood Parameters of Formaldehyde Bath which was Applıed to Rainbow Trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    Hakan BAYRAM

    2017-06-01

    Full Text Available In this study, it was researched the effects of formaldehyde that used commonly at treatment of fish diseases on hematological parameters of rainbow trout (Oncorhynchus mykiss. According to the result of this study, it was found statisticaly significant (p<0.05 difference among of the hematocrit values that measured at the doses x the time interaction. From the point of view of the hemoglobin amount, the application of doses and the dose x time interaction was very significant (p<0.01 and effect of the application of time was significant (p<0.05. From the point of view of reticulocyte proportion, the application of doses and the application of time interaction was very significant (p<0.01.

  2. The influence of altitude and landforms on some biochemical and hematological parameters in Ouled Djellal ewes from arid area of South East Algeria

    Directory of Open Access Journals (Sweden)

    Mohammed Titaouine

    2015-01-01

    Full Text Available Aim: This study was conducted on Ouled Djellal ewes in arid area of south-east Algeria in order to reveal the influence of altitude and landforms on some hematological and biochemical parameters. Materials and Methods: A total of 160 ewes having 3-5 years of age, multiparous, non-pregnant, non-lactating and reared in arid areas of South East Algeria were included. Blood samples were divided according to factors of altitude and landform (plain region at 150 m above sea level, tableland region at 600 m above sea level and mountain region at 1000 m above sea level. The whole blood was analyzed for hematology, and plasma samples for biochemical analysis. Results: The study found lowest glucose concentrations were detected in tableland region at 600 m. In plain region at 150 m, ewes had a higher (p<0.01 concentration of cholesterol and triglyceride. Furthermore, a higher concentration of total proteins (p<0.01 and urea (p<0.05 were detected in plain region at 150 m. The average blood creatinine concentration in mountain ewes at 1000 m and tableland ewes at 600 m were higher (p<0.05 that in plain ewes at 150 m. The highest calcium concentration was found at the altitude of 150 m and the lowest at the altitude of 1000 m (1.12±0.35 mmol/L vs. 0.52±0.03 mmol/L. Phosphorus levels were higher at altitudes of 150 m than at the altitude of 600 m and 1000 m (0.93±0.42 mmol/L vs. 0.68±0.54 mmol/L, 0.23±0.01 mmol/L. The highest hemoglobin concentration and value of hematocrit were detected in mountain ewes at the altitude of 1000 m (120.61 g/L, 40% and the lowest at the altitude of 150 m (73.2 g/L, 31% (p<0.001. Conclusion: We concluded that hematological and biochemical parameters in Ouled Djellel ewes reared in arid area may be affected by altitude and landforms.

  3. Effects of vitamins E and Riboflavin (B2 and combinations of them on the hematological parameters of common carp, Cyprinus carpio L., fingerlings

    Directory of Open Access Journals (Sweden)

    Sharifzadeh Seyedeh Atefeh

    2015-06-01

    Full Text Available In the present study, the effects of vitamins E, B2 and combinations of them on hematological parameters of common carp, Cyprinus carpio L., were investigated during a 56-day experiment. The various dietary levels of vitamins E, B2 and their combination were used as experimental treatments, as follows: T1: vitamin E (80 mg, T2: vitamin E (160 mg kg diet-1, T3: vitamin E (240 mg kg diet-1, T4: vitamin B2 (7 mg kg diet-1, T5: vitamin B2 (15 mg kg diet-1, T6: vitamin B2 (20 mg kg diet-1, T7: vitamin E (80 mg kg diet-1 + vitamin B2 (7 mg kg diet-1, T8: vitamin E (160 mg kg diet-1 + vitamin B2 (15 mg kg diet-1, T9: vitamin E (240 mg kg diet-1 + vitamin B2 (20 mg kg diet-1. One group not given vitamin supplements was the control. The values of red blood cells (RBC, hemoglobin (Hb, and hematocrit (Hct were higher in T7 than in the other experimental treatments. The values of white blood cells (WBC were higher in T2 than in the other treatments. The concentration of immunoglobulin (IgM was also lower in T10 than in the other experimental treatments. The highest values of mean corpuscular hemoglobin (MCH and mean corpuscular volume (MCV were observed in T2. No significant differences were noted among experimental groups in the values of mean corpuscular hemoglobin concentration (MCHC. This study showed that vitamin E and B2 supplements alter the hematological parameters of common carp.

  4. Hematology of camelids.

    Science.gov (United States)

    Vap, Linda; Bohn, Andrea A

    2015-01-01

    Interpretation of camelid hematology results is similar to that of other mammals. Obtaining accurate results and using appropriate reference intervals can be a bit problematic, particularly when evaluating the erythron. Camelid erythrocytes vary from other mammals in that they are small, flat, and elliptical. This variation makes data obtained from samples collected from these species prone to error when using some automated instruments. Normal and abnormal findings in camelid blood are reviewed as well as how to ensure accurate results.

  5. Analysis of waveform-derived ORA parameters in early forms of keratoconus and normal corneas.

    Science.gov (United States)

    Ventura, Bruna V; Machado, Aydano P; Ambrósio, Renato; Ribeiro, Guilherme; Araújo, Luana N; Luz, Allan; Lyra, João Marcelo

    2013-09-01

    To evaluate the Ocular Response Analyzer (ORA; Reichert Ophthalmic Instruments, Depew, NY) performance in differentiating grades I and II keratoconus from normal corneas using 41 parameters individually and to assess the effect of analyzing all parameters together. This study compared the mean value of 41 ORA parameters in grades I and II keratoconus with healthy age-matched control eyes. Only eyes with a central corneal thickness between 500 and 600 μm were included. The area under the receiver operating characteristic curve was calculated for each of the 41 parameters independently and for all of the parameters together. This study included 136 eyes with normal corneas and 68 eyes with grades I and II keratoconus. When analyzed individually, four ORA parameters (p1area, p1area1, p2area, and p2area1) had an area under the curve greater than 0.900 for discriminating between both groups. The p2area was the parameter that achieved the largest area under the curve individually (0.931). The area under the curve increased to 0.978 when analyzing all parameters together. Alternative ORA parameters are better for differentiating grades I and II keratoconus from normal corneas than the four parameters originally available for ophthalmologists (corneal hysteresis, Goldmann-correlated intraocular pressure, corneal-compensated intraocular pressure, and corneal resistance factor). Although the ORA did not achieve 100% accuracy, the discrimination between these two groups was optimized by combining all parameters. Copyright 2013, SLACK Incorporated.

  6. Extended helical tomo-therapy and concomitant chemotherapy for an uterine cervix carcinoma: dosimetry parameters and hematological toxicity; Tomotherapie helicoidale etendue et chimiotherapie concomitante pour un cancer du col de l'uterus: parametres dosimetriques et toxicite hematologique

    Energy Technology Data Exchange (ETDEWEB)

    Haddad, H.; Thomas, L.; Belhomme, S.; Chemin, A.; Caron, J.; Dejean, C.; Kantor, G.; Richaud, P. [Institut Bergonie, Dept. de Radiotherapie, 33 - Bordeaux (France); Floquet, A.; Guyon, F. [Institut Bergonie, Dept. de Chirurgie, 33 - Bordeaux (France)

    2009-10-15

    the extended tomo-therapy associated to concomitant chemotherapy is feasible and allows a dose escalation at the ganglions level. It is necessary to continue to study the dosimetry parameters at the hematopoietic marrow level that are predictive for a hematological toxicity. (N.C.)

  7. Effect of Short-Term High Fat Diet Inducing Obesity on Hematological, Some Biochemical Parameters and Testicular Oxidative Stress in Male Rats

    Directory of Open Access Journals (Sweden)

    Sherif M. Shawky

    2015-10-01

    Full Text Available Obesity constitutes a health problem due to its increasing worldwide prevalence. Among the health detriments caused by obesity, reproduction is disrupted. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. The objective of the current work was to examine the effect of diet-induced obesity on blood parameters, liver and kidney function tests, lipid profile and testicular oxidative stress. For that purpose, Male rats were fed ad libitum with a standard diet (control group; n.= 15 and high fat diet (HFD group; n.= 15 for 6 weeks. Hematological parameters, urea, creatinine, albumin were similar between the two groups. Intergroup testosterone levels were also comparable. The high fat diet induced significant increase in serum triglycerides, cholesterol, low density lipoprotein and very low density lipoprotein cholesterol concentrations. This diet also increases significantly alanine aminotransferase and aspartate aminotransferase activities and decreased total protein level and high-density lipoprotein cholesterol concentration. Furthermore, HFD showed a significant increasing in malondialdehyde contents in testes and decreasing in superoxide dismutase activity, the results of this study concluded that short-term high fat diet affect on liver enzymes and causing oxidative stress in testes.

  8. Estimator of a non-Gaussian parameter in multiplicative log-normal models

    Science.gov (United States)

    Kiyono, Ken; Struzik, Zbigniew R.; Yamamoto, Yoshiharu

    2007-10-01

    We study non-Gaussian probability density functions (PDF’s) of multiplicative log-normal models in which the multiplication of Gaussian and log-normally distributed random variables is considered. To describe the PDF of the velocity difference between two points in fully developed turbulent flows, the non-Gaussian PDF model was originally introduced by Castaing [Physica D 46, 177 (1990)]. In practical applications, an experimental PDF is approximated with Castaing’s model by tuning a single non-Gaussian parameter, which corresponds to the logarithmic variance of the log-normally distributed variable in the model. In this paper, we propose an estimator of the non-Gaussian parameter based on the q th order absolute moments. To test the estimator, we introduce two types of stochastic processes within the framework of the multiplicative log-normal model. One is a sequence of independent and identically distributed random variables. The other is a log-normal cascade-type multiplicative process. By analyzing the numerically generated time series, we demonstrate that the estimator can reliably determine the theoretical value of the non-Gaussian parameter. Scale dependence of the non-Gaussian parameter in multiplicative log-normal models is also studied, both analytically and numerically. As an application of the estimator, we demonstrate that non-Gaussian PDF’s observed in the S&P500 index fluctuations are well described by the multiplicative log-normal model.

  9. A Retrospective Mid- and Long-term Follow-up Study on the Changes in Hematologic Parameters in the Highly Exposed Residents of the Hebei Spirit Oil Spill in Taean, South Korea.

    Science.gov (United States)

    Choi, Young-Hyun; Hong, Jee-Young; Lee, Moo-Sik

    2017-10-01

    This study aimed to analyze changes in hematologic parameters in the residents of the areas highly contaminated by the Hebei Spirit Oil Spill in 2007 and those who participated in the clean-up activities. According to demographic characteristics, health status and behavior, and level of exposure to oil, we compared the hematologic results in 2009 and 2012 among 701 residents. The hematologic parameters were composed of white blood cell (WBC) count, and levels of hemoglobin, hematocrit (Hct), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, glycosylated hemoglobin (HbA1c), blood urea nitrogen (BUN), creatinine (Cr), total cholesterol (T-chol), high-density lipoprotein (HDL), and triglyceride (TG). Paired t -test revealed that the WBC count and levels of Hct, AST, ALT, glucose, and HbA1c significantly increased, whereas the BUN, Cr, HDL, and TG levels significantly decreased. Multiple linear regression modelling showed a relationship between the level of exposure to oil and temporal changes in Hct, glucose, HbA1c, and BUN levels. Our results suggest a relationship between level of exposure to oil and changes in hematologic parameters over 3 years. Further studies should be conducted to determine the impact of oil spill on health such as the occurrence of diseases.

  10. A Retrospective Mid- and Long-term Follow-up Study on the Changes in Hematologic Parameters in the Highly Exposed Residents of the Hebei Spirit Oil Spill in Taean, South Korea

    Science.gov (United States)

    Choi, Young-Hyun; Hong, Jee-Young; Lee, Moo-Sik

    2017-01-01

    Objectives This study aimed to analyze changes in hematologic parameters in the residents of the areas highly contaminated by the Hebei Spirit Oil Spill in 2007 and those who participated in the clean-up activities. Methods According to demographic characteristics, health status and behavior, and level of exposure to oil, we compared the hematologic results in 2009 and 2012 among 701 residents. The hematologic parameters were composed of white blood cell (WBC) count, and levels of hemoglobin, hematocrit (Hct), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, glycosylated hemoglobin (HbA1c), blood urea nitrogen (BUN), creatinine (Cr), total cholesterol (T-chol), high-density lipoprotein (HDL), and triglyceride (TG). Results Paired t-test revealed that the WBC count and levels of Hct, AST, ALT, glucose, and HbA1c significantly increased, whereas the BUN, Cr, HDL, and TG levels significantly decreased. Multiple linear regression modelling showed a relationship between the level of exposure to oil and temporal changes in Hct, glucose, HbA1c, and BUN levels. Conclusion Our results suggest a relationship between level of exposure to oil and changes in hematologic parameters over 3 years. Further studies should be conducted to determine the impact of oil spill on health such as the occurrence of diseases. PMID:29164048

  11. Effects of intramammary infusion of sage (Salvia officinalis essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis

    Directory of Open Access Journals (Sweden)

    Myassar O. Alekish

    2017-08-01

    Full Text Available Aim: The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis essential oil (EO on milk somatic cell count (SCC, milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. Materials and Methods: The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5: Dimethyl sulfoxide (DMSO alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline, Group 2 (n=5: Amoxicillin alone (3 ml, and Group 3 (n=10: Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]. All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0 and at 24 (T24 and 48 (T48 h after the last treatment. Results: The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (p<0.05 at T24 and T48 h in sage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (p<0.05 in sage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations

  12. Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis.

    Science.gov (United States)

    Alekish, Myassar O; Ismail, Zuhair B; Awawdeh, Mofleh S; Shatnawi, Shoroq

    2017-08-01

    The aims of this study were to evaluate the effects of intramammary infusion of sage ( Salvia officinalis ) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline), Group 2 (n=5): Amoxicillin alone (3 ml), and Group 3 (n=10): Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]). All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0) and at 24 (T24) and 48 (T48) h after the last treatment. The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (psage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (psage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations. In this study, the intramammary infusion of sage EO to ewes affected with

  13. Effect of acute exposure to nonylphenol on biochemical, hormonal, and hematological parameters and muscle tissues residues of Nile tilapia; Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Hager Tarek H. Ismail

    2016-06-01

    Full Text Available Aim: This study is aimed to evaluate some biochemical, hormonal, hematological, and histopathological changes in Nile tilapia, Oreochromis niloticus, after acute exposure to nonylphenol (NP. In addition to detection of NP residues in the fish, muscle tissues for human health concern. Materials and Methods: A total of 90 apparently healthy Nile tilapia, O. niloticus, were randomly divided into three equal groups; each containing 30 fish (three replicates. Groups 1 and 2 kept as a control and solvent control (acetone, respectively, and Group 3 exposed to NP at a dose level of 500 μg/L water for 7 successive days. Blood and tissue samples were collected 2 times randomly from each group after 7 days from fish exposure to NP and 10 days from exposure stopping. Results: Fish exposed to NP Group 3 showed anorexia, sluggish movement, erythema of the skin, areas of scales loss, and hemorrhagic ulcers in some areas of body region leading to exposing the viscera. Biochemical results revealed a significant increase in serum total proteins and globulins levels, a highly significant increase in serum alanine aminotransferase and aspartate aminotransferase activities, triglycerides, cholesterol, and creatinine levels, insignificant increase in serum uric acid level, and a highly significant decrease in serum testosterone and estradiol-β17 levels in Group 3 in compare with the control group. Histopathological finding confirms these results. While hematological results of the same group revealed a significant increase in red blood cells count and packed cell volume value, insignificant increase in hemoglobin concentration, leukopenia, lymphopenia, and monocytopenia in compared with the control group. All of these changes appeared after 7 days from fish exposure to NP. Most of these alterations returned toward the normal level after 10 days from stopping exposure to NP. NP residues detected in fish muscle tissues of Group 3 during exposure and after stopping

  14. Effect of acute exposure to nonylphenol on biochemical, hormonal, and hematological parameters and muscle tissues residues of Nile tilapia; Oreochromis niloticus.

    Science.gov (United States)

    Ismail, Hager Tarek H; Mahboub, Heba Hassan H

    2016-06-01

    This study was aimed to evaluate some biochemical, hormonal, hematological, and histopathological changes in Nile tilapia, Oreochromis niloticus, after acute exposure to nonylphenol (NP). In addition to detection of NP residues in the fish, muscle tissues for human health concern. A total of 90 apparently healthy Nile tilapia, O. niloticus, were randomly divided into three equal groups; each containing 30 fish (three replicates). Groups 1 and 2 kept as a control and solvent control (acetone), respectively, and Group 3 exposed to NP at a dose level of 500 µg/L water for 7 successive days. Blood and tissue samples were collected 2 times randomly from each group after 7 days from fish exposure to NP and 10 days from exposure stopping. Fish exposed to NP Group 3 showed anorexia, sluggish movement, erythema of the skin, areas of scales loss, and hemorrhagic ulcers in some areas of body region leading to exposing the viscera. Biochemical results revealed a significant increase in serum total proteins and globulins levels, a highly significant increase in serum alanine aminotransferase and aspartate aminotransferase activities, triglycerides, cholesterol, and creatinine levels, insignificant increase in serum uric acid level, and a highly significant decrease in serum testosterone and estradiol-β17 levels in Group 3 in compare with the control group. Histopathological finding confirms these results. While hematological results of the same group revealed a significant increase in red blood cells count and packed cell volume value, insignificant increase in hemoglobin concentration, leukopenia, lymphopenia, and monocytopenia in compared with the control group. All of these changes appeared after 7 days from fish exposure to NP. Most of these alterations returned toward the normal level after 10 days from stopping exposure to NP. NP residues detected in fish muscle tissues of Group 3 during exposure and after stopping exposure to it. It is concluded that NP is a toxic

  15. Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

    Directory of Open Access Journals (Sweden)

    Reka Tienken

    2015-09-01

    Full Text Available The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010 serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL, whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of energy to cows which were similar in body condition score had only minor effects on periparturient immune system function and the metabolism of those cows.

  16. Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802).

    Science.gov (United States)

    Yu, Wei; Wen, Guoliang; Lin, Heizhao; Yang, Yukai; Huang, Xiaolin; Zhou, Chuanpeng; Zhang, Zaiwang; Duan, Yafei; Huang, Zhong; Li, Tao

    2018-03-01

    The present study investigated the effects of dietary Spirulina platensis supplementation on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and resistance to the pathogen infection in Coral trout Plectropomus leopardus. The fish were fed for 8-week with diets containing different levels of S. platensis: 0% (C), 2% (SP2), 4% (SP4), 6% (SP6), 8% (SP8) and 10% (SP10) as treatment groups, followed by a Vibrio harveyi infection test for 14 d. The study indicated that dietary supplementation with Spirulina platensis could significantly improve growth performance, and the highest weight gain rate (WGR) and specific growth rate (SGR) were observed in group SP10 (P platensis supplemented groups were significantly higher than those of group C (P platensis levels. Compared with group C, the lysozyme (LYZ) and respiratory burst activities (RBA), and immunoglobulin (Ig) and complement contents in group SP4, SP6, SP8 and SP10 increased significantly than those of group C respectively (P platensis (especially at 10%) could significantly promote its growth performance, improve its hepatic antioxidant status, and enhance its immune ability and resistance to V. harveyi infection. Copyright © 2018 Elsevier Ltd. All rights reserved.

  17. Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide.

    Science.gov (United States)

    Ghorbel, Imen; Maktouf, Sameh; Kallel, Choumous; Ellouze Chaabouni, Semia; Boudawara, Tahia; Zeghal, Najiba

    2015-07-05

    The individual toxic effects of aluminium and acrylamide are well known but there are no data on their combined effects. The present study was undertaken to determine (i) hematological parameters during individual and combined chronic exposure to aluminium and acrylamide (ii) correlation of oxidative stress in erythrocytes with pro-inflammatory cytokines expression, DNA damage and histopathological changes in the liver. Rats were exposed to aluminium (50 mg/kg body weight) in drinking water and acrylamide (20 mg/kg body weight) by gavage, either individually or in combination for 3 weeks. Exposure rats to AlCl3 or/and ACR provoked an increase in MDA, AOPP, H2O2 and a decrease in GSH and NPSH levels in erythrocytes. Activities of catalase, glutathione peroxidase and superoxide dismutase were decreased in all treated rats. Our results showed that all treatments induced an increase in WBC, erythrocyte osmotic fragility and a decrease in RBC, Hb and Ht. While MCV, MCH, MCHC remained unchanged. Hepatic pro-inflammatory cytokines expression including tumor necrosis factor-α, interleukin-6, interleukin-1β was increased suggesting leucocytes infiltration in the liver. A random DNA degradation was observed on agarose gel only in the liver of co-exposed rats to AlCl3 and ACR treatment. Interestingly, co-exposure to these toxicants exhibited synergism based on physical and biochemical variables in erythrocytes, pro-inflammatory cytokines and DNA damage in liver. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  18. Effect of gamma radiation on the growth, survival, hematology and histological parameters of rainbow trout (Oncorhynchus mykiss) larvae

    Energy Technology Data Exchange (ETDEWEB)

    Oujifard, Amin, E-mail: oujifard.amin@gmail.com [Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Borazjan, Bushehr (Iran, Islamic Republic of); Amiri, Roghayeh [Department of Veterinary, Agricultural Medical and Industrial Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj (Iran, Islamic Republic of); Shahhosseini, Gholamreza [Fisheries Department, Faculty of Natural Resources and Marine Sciences, TarbiatModares University, Noor, Mazandaran (Iran, Islamic Republic of); Davoodi, Reza [Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Borazjan, Bushehr (Iran, Islamic Republic of); Moghaddam, Jamshid Amiri [Fisheries Department, Faculty of Natural Resources and Marine Sciences, TarbiatModares University, Noor, Mazandaran (Iran, Islamic Republic of)

    2015-08-15

    Highlights: • Incrementing of gamma radiation reveals the negative effects on fish larvae. • Radiation adversely affected the weight, blood cells and intestinal morphology of the larvae. • No mortality was observed at low dosage of gamma radiation on fish larvae. - Abstract: Effects of low (1, 2.5 and 5 Gy) and high doses (10, 20 and 40 Gy) of gamma radiation were examined on the growth, survival, blood parameters and morphological changes of the intestines of rainbow trout (Oncorhynchus mykiss) larvae (103 ± 20 mg) after 12 weeks of exposure. Negative effects of gamma radiation on growth and survival were observed as radiation level and time increased. Changes were well documented at 10 and 20 Gy. All the fish were dead at the dose of 40 Gy. In all the treatments, levels of red blood cells (RBC), hematocrit (HCT) and hemoglobin (HB) were significantly (P < 0.05) declined as the irradiation levels increased, whereas the amount of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) did not change. No significant differences (P > 0.05) were found in the levels of white blood cells (WBC), lymphocytes and monocytes. Destruction of the intestinal epithelium cells was indicated as the irradiation levels increased to 1 Gy and above. The highest levels of growth, survival, specific growth rate (SGR), condition factor (CF) and protein efficiency rate (PER) were obtained in the control treatment. The results showed that gamma rays can be a potential means for damaging rainbow trout cells.

  19. Effect of gamma radiation on the growth, survival, hematology and histological parameters of rainbow trout (Oncorhynchus mykiss) larvae

    International Nuclear Information System (INIS)

    Oujifard, Amin; Amiri, Roghayeh; Shahhosseini, Gholamreza; Davoodi, Reza; Moghaddam, Jamshid Amiri

    2015-01-01

    Highlights: • Incrementing of gamma radiation reveals the negative effects on fish larvae. • Radiation adversely affected the weight, blood cells and intestinal morphology of the larvae. • No mortality was observed at low dosage of gamma radiation on fish larvae. - Abstract: Effects of low (1, 2.5 and 5 Gy) and high doses (10, 20 and 40 Gy) of gamma radiation were examined on the growth, survival, blood parameters and morphological changes of the intestines of rainbow trout (Oncorhynchus mykiss) larvae (103 ± 20 mg) after 12 weeks of exposure. Negative effects of gamma radiation on growth and survival were observed as radiation level and time increased. Changes were well documented at 10 and 20 Gy. All the fish were dead at the dose of 40 Gy. In all the treatments, levels of red blood cells (RBC), hematocrit (HCT) and hemoglobin (HB) were significantly (P < 0.05) declined as the irradiation levels increased, whereas the amount of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) did not change. No significant differences (P > 0.05) were found in the levels of white blood cells (WBC), lymphocytes and monocytes. Destruction of the intestinal epithelium cells was indicated as the irradiation levels increased to 1 Gy and above. The highest levels of growth, survival, specific growth rate (SGR), condition factor (CF) and protein efficiency rate (PER) were obtained in the control treatment. The results showed that gamma rays can be a potential means for damaging rainbow trout cells

  20. The effect of Zataria multiflora on pulmonary function tests, hematological and oxidant/antioxidant parameters in sulfur mustard exposed veterans, a randomized doubled-blind clinical trial.

    Science.gov (United States)

    Khazdair, Mohammad Reza; Rajabi, Omid; Balali-Mood, Mahdi; Beheshti, Farimah; Boskabady, Mohammad Hossein

    2018-03-01

    Sulfur mustard is an alkylating agent which cause to short and long term incapacitations on various organs including lung. There is no definite treatment for lung disorders induced by SM exposure. In the present study, the preventive effect of Zataria multiflora (Z. multiflora) on hematological parameters, oxidant/antioxidant markers and pulmonary function tests (PFT) in veterans, 27-30 years after exposed to SM were studied. Forty seven veterans allocated to three groups included: placebo group (P) and two groups treated with 5 and 10 mg/kg/day of Z. multiflora (Zat 5 and Zat 10). Drugs were prescribed in a double-blind manner for two months. Total and different WBC, hematological indices, oxidant/antioxidant markers and PFT values included; force vital capacity (FVC) and peak expiratory flow (PEF) were assessed at the beginning (step 0), one and two month (step I and II, respectively) after starting treatment. Total and different white blood cell in Zat 5 and 10 mg/kg treated groups in Step I and II were significantly decreased compared to Step 0 (p < 0.05 to p < 0.001). The levels of thiol, superoxide dismutase (SOD) and catalase (CAT) in Zat 5 and 10 mg/kg treated groups in step I and II were significantly increased (p < 0.05 to p < 0.001) but the level of malondialdehyde (MDA) significantly decreased in two treatment groups compared to Step 0 (p < 0.05 and p < 0.001 respectively). FVC and PEF values were significant increase in Zat 5 and 10 mg/kg treated groups in step I and II compared to step 0 (p < 0.05 to p < 0.001). Furthermore, FVC and PEF values in Zat 5 mg/kg were also increased in step II compared to step I (p < 0.01 for both). The percentage improvement of total and differential WBC, oxidant/antioxidant markers, FVC and PEF values during two moth treatment period significantly improved in the treated groups compared to the placebo group. Z. multiflora reduces inflammatory cells and oxidant biomarkers

  1. House sparrow biomarkers as lead pollution bioindicators. Evaluation of dose and exposition length on hematological and oxidative stress parameters.

    Science.gov (United States)

    Cid, Fabricio D; Fernández, Noelia C; Pérez-Chaca, María V; Pardo, Rafael; Caviedes-Vidal, Enrique; Chediack, Juan G

    2018-06-15

    House sparrows (Passer domesticus) have been proposed as a key ecological indicator of urban pollution. Remarkably, we lack knowledge about the physiological effects of lead on this bird species. Therefore, this study was aimed to evaluate the effect of Pb on several physiological parameters in house sparrows exposed to environmental Pb concentrations. In a first experiment, birds were exposed to Pb sub-lethal doses (from 1.3 to 14.0 µg of Pb/g animal/day) during 5 days, which resulted in a dose response increase of blood Pb levels and decrease of blood ALAD activity. However, at the higher doses tested (> 7 μg of Pb/g animal/day) the blood ALAD activity inhibition (~82%) remained constant. Hematocrit and hemoglobin were significantly reduced only at the highest-doses, and the stress indicator, heterophils to lymphocyte (H/L) ratio, did not show apparent changes. In a second experiment, house sparrows were exposed to Pb in drinking water (12.3 ppm) during either 15 or 30 days. Pb concentration used in this study was enough to produce blood lead levels equivalents to those found recently in house sparrows inhabiting urban areas, reduced blood ALAD activity and inversion of the H/L ratio. Decreasing blood ALAD activities were correlated with increasing blood Pb levels. In addition, Pb exposure produced modification in the levels of hepatic antioxidant enzymes, increased GST activity and decreased CAT activity, without lipid peroxidation. In conclusion, our results suggest that blood ALAD activity is a reliable and sensitive biomarker for environmental Pb exposure in house sparrows, additionally chronic exposure produce physiological stress (H/L inversion) and small changes in antioxidant enzyme activity. Finally, this specie could be considered a bioindicator for monitoring the urban Pb contamination. Copyright © 2018 Elsevier Inc. All rights reserved.

  2. GRAVIMETRIC-DETERMINATION OF THE WATER CONCENTRATION IN WHOLE-BLOOD, PLASMA AND ERYTHROCYTES AND CORRELATIONS WITH HEMATOLOGICAL AND CLINICOCHEMICAL PARAMETERS

    NARCIS (Netherlands)

    LIJNEMA, TH; HUIZENGA, [No Value; JAGER, J; MACKOR, AJ; GIPS, CH

    1993-01-01

    We have assessed gravimetric methods for determination of intravascular water, established whole blood-, plasma- and erythrocyte water reference values in a healthy volunteer group (n = 97, 48 females) and correlated these variables with 30 simultaneous hematological, clinicochemical and body

  3. Toxic effects and depuration after the dietary lead(II) exposure on the bioaccumulation and hematological parameters in starry flounder (Platichthys stellatus).

    Science.gov (United States)

    Hwang, In-Ki; Kim, Kyeong-Wook; Kim, Jun-Hwan; Kang, Ju-Chan

    2016-07-01

    Platichthys stellatus (mean length 20±2cm, mean weight 160.15±15g) were exposed to the different levels of dietary lead(II) at the concentrations of 0, 30, 60, 120, 240mg/kg for 4 weeks. Depuration was conducted for 2 weeks after exposure. The lead exposure over 60mg Pb/kg induced the significant bioaccumulation in tissues of P. stellatus (5-30μg/g tissue), except for brain and muscle where the exposure to 240mg Pb/kg caused the bioaccumulation (2-4μg/g tissue). The hematological parameters such as red blood cell (RBC) counts, hematocrit (Ht) value and hemoglobin (Hb) concentration were substantially decreased over 60mg Pb/kg, and lasted even after the depuration period. For plasma components, calcium and magnesium levels in plasma were generally decreased over 60mg Pb/kg, and glucose level was also mainly increased over 60mg Pb/kg. Total protein was significantly decreased over 120mg Pb/kg after 4 weeks exposure. Glucose and total protein showed the restoration after the depuration period in groups of fish exposed previously to over 60 and 120mg Pb/kg, respectively. However, other parameters that changed during the exposure over 60mg Pb/kg did not recovered. For enzymatic components in plasma, glutamic oxalate transminase (GOT), glutamic pyruvate transminase (GPT) and alkaline phosphatase (ALP) were significantly increased over 120mg Pb/kg, and there was only restoration observed after the depuration for ALP over 120mg Pb/kg. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. Biochemical and hematological profile of different breeds of goat ...

    African Journals Online (AJOL)

    shthomas

    does of all breeds were within the normal range for white blood cells (8.05-12.88 × 103/µL) and red blood cell (10.44-12.65 × 106/µL). ... CP, Crude protein; EE, ether extract; ADF, acid detergent fiber; NDF, neutral detergent fiber. ... effects of breed on biochemical parameters and hematology profiles of does were analyzed ...

  5. ORA waveform-derived biomechanical parameters to distinguish normal from keratoconic eyes

    Directory of Open Access Journals (Sweden)

    Allan Luz

    2013-04-01

    Full Text Available PURPOSE: To evaluate the ability of the Ocular Response Analyzer (ORA; Reichert Ophthalmic Instruments, Buffalo, NY to distinguish between normal and keratoconic eyes, by comparing pressure and waveform signal-derived parameters. METHODS: This retrospective comparative case series study included 112 patients with normal corneas and 41 patients with bilateral keratoconic eyes. One eye from each subject was randomly selected for analysis. Keratoconus diagnosis was based on clinical examinations, including Placido disk-based corneal topography and rotating Scheimpflug corneal tomography. Data from the ORA best waveform score (WS measurements were extracted using ORA software. Corneal hysteresis (CH, corneal resistance factor (CRF, Goldman-correlated intraocular pressure (IOPg, cornea-compensated intraocular pressure (IOPcc, and 37 parameters derived from the waveform signal were analyzed. Differences in the distributions among the groups were assessed using the Mann-Whitney test. Receiver operating characteristic (ROC curves were calculated. RESULTS: Statistically significant differences between keratoconic and normal eyes were found in all parameters (p<0.05 except IOPcc and W1. The area under the ROC curve (AUROC was greater than 0.85 for 11 parameters, including CH (0.852 and CRF (0.895. The parameters related to the area under the waveform peak during the second and first applanations (p2area and p1area had the best performances, with AUROCs of 0.939 and 0.929, respectively. The AUROCs for CRF, p2area, and p1area were significantly greater than that for CH. CONCLUSION: There are significant differences in biomechanical metrics between normal and keratoconic eyes. Compared with the pressure-derived parameters, corneal hysteresis and corneal resistance factor, novel waveform-derived ORA parameters provide better identification of keratoconus.

  6. ORA waveform-derived biomechanical parameters to distinguish normal from keratoconic eyes.

    Science.gov (United States)

    Luz, Allan; Fontes, Bruno Machado; Lopes, Bernardo; Ramos, Isaac; Schor, Paulo; Ambrósio, Renato

    2013-01-01

    To evaluate the ability of the Ocular Response Analyzer (ORA; Reichert Ophthalmic Instruments, Buffalo, NY) to distinguish between normal and keratoconic eyes, by comparing pressure and waveform signal-derived parameters. This retrospective comparative case series study included 112 patients with normal corneas and 41 patients with bilateral keratoconic eyes. One eye from each subject was randomly selected for analysis. Keratoconus diagnosis was based on clinical examinations, including Placido disk-based corneal topography and rotating Scheimpflug corneal tomography. Data from the ORA best waveform score (WS) measurements were extracted using ORA software. Corneal hysteresis (CH), corneal resistance factor (CRF), Goldman-correlated intraocular pressure (IOPg), cornea-compensated intraocular pressure (IOPcc), and 37 parameters derived from the waveform signal were analyzed. Differences in the distributions among the groups were assessed using the Mann-Whitney test. Receiver operating characteristic (ROC) curves were calculated. Statistically significant differences between keratoconic and normal eyes were found in all parameters (p<0.05) except IOPcc and W1. The area under the ROC curve (AUROC) was greater than 0.85 for 11 parameters, including CH (0.852) and CRF (0.895). The parameters related to the area under the waveform peak during the second and first applanations (p2area and p1area) had the best performances, with AUROCs of 0.939 and 0.929, respectively. The AUROCs for CRF, p2area, and p1area were significantly greater than that for CH. There are significant differences in biomechanical metrics between normal and keratoconic eyes. Compared with the pressure-derived parameters, corneal hysteresis and corneal resistance factor, novel waveform-derived ORA parameters provide better identification of keratoconus.

  7. Normalization of informatisation parameter on airfield light-signal bar at flights in complex meteorological conditions

    Directory of Open Access Journals (Sweden)

    П.В. Попов

    2005-03-01

    Full Text Available  The technique of maintenance of the set level of flights safetivness is developed by normalization of informatisation parameters functional groups of light-signal lightings at technological stages of interaction of crew of the airplane with the airfield light-signals bar at flights in a complex weathercast conditions.

  8. Normalization.

    Science.gov (United States)

    Cuevas, Eduardo J.

    1997-01-01

    Discusses cornerstone of Montessori theory, normalization, which asserts that if a child is placed in an optimum prepared environment where inner impulses match external opportunities, the undeviated self emerges, a being totally in harmony with its surroundings. Makes distinctions regarding normalization, normalized, and normality, indicating how…

  9. Correlation of normal-range FMR1 repeat length or genotypes and reproductive parameters.

    Science.gov (United States)

    Maslow, Bat-Sheva L; Davis, Stephanie; Engmann, Lawrence; Nulsen, John C; Benadiva, Claudio A

    2016-09-01

    This study aims to ascertain whether the length of normal-ranged CGG repeats on the FMR1 gene correlates with abnormal reproductive parameters. We performed a retrospective, cross-sectional study of all FMR1 carrier screening performed as part of routine care at a large university-based fertility center from January 2011 to March 2014. Correlations were performed between normal-range FMR1 length and baseline serum anti-Müllerian hormone (AMH), cycle day 3 follicle stimulating hormone (FSH), ovarian volumes (OV), antral follicle counts (AFC), and incidence of diminished ovarian reserve (DOR), while controlling for the effect of age. Six hundred three FMR1 screening results were collected. One subject was found to be a pre-mutation carrier and was excluded from the study. Baseline serum AMH, cycle day 3 FSH, OV, and AFC data were collected for the 602 subjects with normal-ranged CGG repeats. No significant difference in median age was noted amongst any of the FMR1 repeat genotypes. No significant correlation or association was found between any allele length or genotype, with any of the reproductive parameters or with incidence of DOR at any age (p > 0.05). However, subjects who were less than 35 years old with low/low genotype were significantly more likely to have below average AMH levels compared to those with normal/normal genotype (RR 3.82; 95 % CI 1.38-10.56). This large study did not demonstrate any substantial association between normal-range FMR1 repeat lengths and reproductive parameters.

  10. Hematological and nutritional parameters in apparently healthy elderly individuals Parâmetros hematológicos e nutricionais em idosos aparentemente saudáveis

    Directory of Open Access Journals (Sweden)

    Mariza D'Agord Schaan

    2007-06-01

    Full Text Available A non-probabilistic study in an elderly free living community was performed. The first analysis was cross-sectional in design and the additional analysis, a case-control design. In the first analysis, 745 subjects were enrolled. Additional analyses, made in 46 apparently healthy elderly subjects (AHE and 33 young adults (YA included: clinical, hematological and biochemical analyses, 24-hours nutritional recall and diet frequency questionnaire. The AHE were selected using the Senieur test protocol. To avoid selection bias in the AHE sample, additional analysis comparing cultural, socioeconomic, and health variables were compared between AHE and non-selected elderly with same dysfunction or morbidity (DE. The prevalence of AHE was 6.17% (n=46 among whom 4.3% were anemic. There were no statistically differences in hematocrit, hemoglobin, leukocytes and vitamin B12 levels between AHE and YA groups. Mean values of MCV, RDW, eosinophils, folate and ferritin were higher in the AHE than the YA group. On the other hand, platelets were higher in the YA group. No statistically significant difference occurred between the AHE and DE groups when nutritional indicators were compared. The comparison between nutritional indicators of anemic and non-anemic apparently healthy elderly people showed statistically significant differences in vitamin B12 and protein intake, which were lower in the anemic elderly. The results suggest independent biological differences between hematological parameters of elderly and young individuals.Foi realizado um estudo não probabilístico em idosos que vivem na comunidade. A primeira análise teve um delineamento transversal e análises adicionais um delineamento caso-controle. Na primeira análise foram incluídos 745 indivíduos. Análises adicionais foram feitas em 46 idosos saudáveis (HE e em 33 adultos jovens (YA incluindo: análises clínicas, hematológicas e bioquímicas, recordatório nutricional de 24 horas e question

  11. Influence of Target Parameters on Fixation Stability in Normal and Strabismic Monkeys.

    Science.gov (United States)

    Pirdankar, Onkar H; Das, Vallabh E

    2016-03-01

    The purpose of this study was to assess the effect of fixation target parameters on fixation instability in strabismic monkeys. One normal and three exotropic monkeys were presented with four differently shaped fixation targets, with three diameters, during monocular or binocular viewing. Fixation targets were white on a black background or vice versa. Binocular eye movements were recorded using the magnetic search coil technique and fixation stability quantified by calculating the bivariate contour ellipse area (BCEA). Fixation instability was greater in all the strabismic monkeys compared with the normal monkey. During monocular viewing, strabismic monkeys showed significantly greater instability in the covered eye compared to the fixating eye. Multifactorial ANOVA suggested statistically significant target parameter influences, although effect sizes were small. Thus, a disk-shaped target resulted in greater instability than other target shapes in the viewing eyes of the normal monkey and two of three strabismic monkeys. A similar target-shape effect was also observed in the covered eye. Least instability was elicited with a 0.5° target in the normal monkey and a 1.0° target in the strabismic monkeys, both in the viewing and the covered eye. Target/background polarity effects were idiosyncratic. In strabismic monkeys, stability of the fixating eye during binocular viewing was not different from the stability of the same eye during monocular viewing. Abnormal drifts and nystagmus contribute to increased fixation instability in strabismic monkeys. Target parameters (shape and size) that influence fixation stability in a normal animal also affected fixation stability in our sample of strabismic monkeys.

  12. Hematological and biochemical parameters of Dorper ewesParâmetros hematológicos e bioquímicos de ovinos da raça Dorper

    Directory of Open Access Journals (Sweden)

    Karina Medici Madureira

    2013-05-01

    Full Text Available In order to establish hematological and biochemical Dorper sheep values and evaluate the influence of sex and age in these determinations, 72 animals were assigned to three experimental groups according to age: Group 1 (G1 consisting of animals up to 12 months of age (n=28, Group 2 (G2 composed of animals from 13 to 36 months (n=37 and Group 3 (G3 for animals from 37 to 60 months (n=7. The animals were still divided in another two groups according to gender: Group 4 (G4 – females (n=57 and Group 5 (G5 – males (n=15. Evaluating the results obtained for hematological and biochemical examinations it was found that the mean values of RBC (13.4 ± 2.5x106/mL, hemoglobin (12.6 ± 1.3 g/dL, packed cell volume (37.5 ± 3.7%, total leukocytes (7116.0 ± 1967.0/mL and segmented neutrophils (4168.0 ± 1501.0/mL were higher in the animals of G1, as well as urea, GGT and ALP (52.0 ± 17.0 mg/dL, 93.0 ± 19.0 IU/L, 257.0 ± 125.0 IU/L, respectively. Comparing the gender, it was found that the values of total leukocytes (7576.0 ± 2082.0/mL, segmented neutrophils (4296.0 ± 1510.0/mL and lymphocytes (2428.0 ± 1084.0/mL were greater in males (G5. The values of creatinine (1.3 ± 0.4 mg/dL, serum total protein (6.3 ± 0.9 g/dL and globulins (3.7 ± 1.0 g/dL were higher in females (G4. Statistical analysis of the results allowed to determine reference values for hematological and biochemical constituents of Dorper sheep breed, and concluded that there were variations in these parameters influenced by gender and different age groups. A fim de estabelecer os valores hematológicos e bioquímicos de ovinos da raça Dorper e avaliar a influência do sexo e idade nestas determinações, 72 animais foram distribuídos em três grupos experimentais de acordo com a faixa etária, sendo o Grupo 1 (G1 composto por animais com até 12 meses de idade (n=28, o Grupo 2 (G2 composto por animais de 13 a 36 meses de idade (n=37 e o Grupo 3 (G3 por animais de 37 a 60 meses de

  13. Comparison of keratometric and pachymetric parameters with Scheimpflug imaging in normal and keratoconic Asian eyes

    Directory of Open Access Journals (Sweden)

    Lim HB

    2014-11-01

    Full Text Available Hou-Boon Lim,1 Gavin Tan,1 Li Lim,1 Hla Myint Htoon2 1Singapore National Eye Centre, Singapore; 2Singapore Eye Research Institute, Singapore Purpose: To evaluate the keratometric and pachymetric parameters of keratoconic corneas of Asian eyes with the Scheimpflug imaging camera. Patients and methods: This is a cross-sectional study of 22 eyes with Amsler-Krumeich stage 1 keratoconus and 48 eyes from normal subjects conducted in a tertiary eye hospital. A rotating Scheimpflug imaging system, the Pentacam, was used to evaluate all eyes for tomographic parameters, as well as pachymetric progression indices (PPI and Ambrósio relational thickness (ART. Results: All PPI and ART parameter values were significantly different between study and control groups. Cornea minimum radius of curvature, absolute distance from corneal apex to thinnest location, as well as the distance from corneal apex to thinnest location in Y-axis also demonstrated statistically significant differences. The mean ART values for keratoconus eyes were 241 µm (ART-maximum and 352 µm (ART-average, falling within previously reported best cutoff values for detecting keratoconus. On receiver operating characteristics curve analysis, the area under the curve values were highest for PPI and ART parameters. Conclusion: There are significant differences in tomographic parameters between stage 1 keratoconic and normal Asian eyes. Pachymetric indices such as the PPI and the ART index can serve as additional tools in differentiating keratoconic from normal eyes. The findings validate the usefulness of the ART in identifying keratoconic eyes in Asians. Keywords: keratoconus, pentacam, corneal tomography, Ambrósio relational thickness

  14. Data analysis strategies for the characterization of normal: superconductor point contacts by barrier strength parameter

    Science.gov (United States)

    Smith, Charles W.; Reinertson, Randal C.; Dolan, P. J., Jr.

    1993-05-01

    The theoretical description by Blonder, Tinkham, and Klapwijk [Phys. Rev. B 25, 4515 (1982)] of the I-V curves of normal: superconductor point contacts encompasses a broad range of experimental behavior, from the tunnel junction case, on the one hand, to the clean metallic microconstriction limit on the other. The theory characterizes point contacts in terms of a single parameter, the barrier strength. The differential conductance of a point contact, at zero bias, as a function of temperature, offers a direct experimental method by which the barrier strength parameter can be evaluated. In view of the full range of phenomena incorporated by this theory, we suggest several different strategies for the evaluation of the barrier strength parameter from data in the low and intermediate barrier strength regimes and for measurements in the low temperature (near T=0 K) and high temperature (near T=Tc) limits.

  15. The normal ranges of cardiovascular parameters in children measured using the Ultrasonic Cardiac Output Monitor.

    Science.gov (United States)

    Cattermole, Giles N; Leung, P Y Mia; Mak, Paulina S K; Chan, Stewart S W; Graham, Colin A; Rainer, Timothy H

    2010-09-01

    The Ultrasonic Cardiac Output Monitor is a noninvasive method of hemodynamic assessment and monitoring in critically ill patients. There are no published reference ranges for normal values in children for this device. This study aimed to establish normal ranges for cardiovascular indices measured using Ultrasonic Cardiac Output Monitor in children aged 0-12 yrs old and to assess interobserver reliability. This was a population-based cross-sectional observational study. Schools and kindergartens in Hong Kong. Chinese children aged up to 12 yrs old. Two operators performed Ultrasonic Cardiac Output Monitor scans on each child together with standard oscillometric measurement of blood pressure and heart rate. Software intrinsic to the Ultrasonic Cardiac Output Monitor device produces values for stroke volume, cardiac output, and systemic vascular resistance. For each parameter, normal ranges were defined as lying between the 2.5th and 97.5th percentiles. Interobserver reliability was assessed with Bland-Altman plots, coefficients of variation, and intraclass correlation. A total of 1,197 Chinese children (55% boys) were scanned. Normal ranges of values for cardiac output, stroke volume, and systemic vascular resistance indices are presented. Interobserver reliability for Ultrasonic Cardiac Output Monitor was superior to that for standard blood pressure and heart rate measurement. This large study presents normal values for cardiovascular indices in children using the Ultrasonic Cardiac Output Monitor with good interobserver reliability.

  16. American Society of Hematology

    Science.gov (United States)

    ... Navigation Account Navigation Main Content American Society of Hematology ASH Store ASH Job Center ASH Apps Share ... youtube linkedin Research In This Section Agenda for Hematology Research Sickle Cell Priorities Lymphoma Roadmap Moonshot Initiative ...

  17. Effects of dietary administration of stinging nettle (Urtica dioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila.

    Science.gov (United States)

    Ngugi, Charles C; Oyoo-Okoth, Elijah; Mugo-Bundi, James; Orina, Paul Sagwe; Chemoiwa, Emily Jepyegon; Aloo, Peninah A

    2015-06-01

    We investigated effects of dietary administration of stinging nettle (Urtica dioica) on growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) against Aeromonas hydrophila. Fish were divided into 4 groups and fed for 4 and 16 weeks with 0%, 1%, 2% and 5% of U. dioica incorporated into the diet. Use of U. dioica in the diet resulted in improved biochemical, hematological and immunological parameters. Among the biochemical parameters; plasma cortisol, glucose, triglyceride and cholesterol decreased while total protein and albumin in fish increased with increasing dietary inclusion of U. dioica. Among the haematology parameters: red blood cell (RBC), white blood cell (WBC) counts, haematocrit (Htc), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and netrophiles increased with increasing dietary inclusion levels of U. dioica, some depending on the fish age. Serum immunoglobulins, lysozyme activity and respiratory burst were the main immunological parameters in the adult and juvenile L. victorianus measured and they all increased with increasing herbal inclusion of U. dioica in the diet. Dietary incorporation of U. dioica at 5% showed significantly higher relative percentage survival (up to 95%) against A. hydrophila. The current results demonstrate that using U. dioica can stimulate fish immunity and make L. victorianus more resistant to bacterial infection (A. hydrophila). Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Hematological changes in opium addicted diabetic rats.

    Science.gov (United States)

    Asadikaram, Gholamreza; Sirati-Sabet, Majid; Asiabanha, Majid; Shahrokhi, Nader; Jafarzadeh, Abdollah; Khaksari, Mohammad

    2013-01-01

    Chronic opioid treatment in animal models has shown to alter hematological parameters. The aim of this study was to evaluate the biological effects of opium on the number of peripheral blood cells and red blood cells (RBCs) indices in diabetic rats. Peripheral blood samples were collected from diabetic, opium-addicted, diabetic opium-addicted and normal male and female rats and hematological parameters were measured. The mean number of white blood cells (WBCs) was significantly higher in diabetic opium-addict females compared to diabetic non-addict female group. In both male and female, the mean number of neutrophils was significantly higher and the mean number of lymphocytes was lower in diabetic opium-addicted rats than those observed in diabetic non-addicted group. In diabetic opium-addicted male group the mean counts of RBC significantly increased as compared with diabetic male group. However, in diabetic addicted female, the mean number of RBCs was significantly lower than diabetic non-addicted female group. In both males and females, the mean number of platelets was significantly lower in diabetic addict rats compared to diabetic non-addict group. Generally, the results indicated that opium addiction has different effects on male and female rats according to the number of WBC, RBC and RBC indices. It could also be concluded that in the opium-addicts the risk of infection is enhanced due to the weakness of immune system as a result of the imbalance effect of opium on the immune cells.

  19. A Soft Parameter Function Penalized Normalized Maximum Correntropy Criterion Algorithm for Sparse System Identification

    Directory of Open Access Journals (Sweden)

    Yingsong Li

    2017-01-01

    Full Text Available A soft parameter function penalized normalized maximum correntropy criterion (SPF-NMCC algorithm is proposed for sparse system identification. The proposed SPF-NMCC algorithm is derived on the basis of the normalized adaptive filter theory, the maximum correntropy criterion (MCC algorithm and zero-attracting techniques. A soft parameter function is incorporated into the cost function of the traditional normalized MCC (NMCC algorithm to exploit the sparsity properties of the sparse signals. The proposed SPF-NMCC algorithm is mathematically derived in detail. As a result, the proposed SPF-NMCC algorithm can provide an efficient zero attractor term to effectively attract the zero taps and near-zero coefficients to zero, and, hence, it can speed up the convergence. Furthermore, the estimation behaviors are obtained by estimating a sparse system and a sparse acoustic echo channel. Computer simulation results indicate that the proposed SPF-NMCC algorithm can achieve a better performance in comparison with the MCC, NMCC, LMS (least mean square algorithms and their zero attraction forms in terms of both convergence speed and steady-state performance.

  20. Normal Values for Heart Electrophysiology Parameters of Healthy Swine Determined on Electrophysiology Study.

    Science.gov (United States)

    Noszczyk-Nowak, Agnieszka; Cepiel, Alicja; Janiszewski, Adrian; Pasławski, Robert; Gajek, Jacek; Pasławska, Urszula; Nicpoń, Józef

    2016-01-01

    Swine are a well-recognized animal model for human cardiovascular diseases. Despite the widespread use of porcine model in experimental electrophysiology, still no reference values for intracardiac electrical activity and conduction parameters determined during an invasive electrophysiology study (EPS) have been developed in this species thus far. The aim of the study was to develop a set of normal values for intracardiac electrical activity and conduction parameters determined during an invasive EPS of swine. The study included 36 healthy domestic swine (24-40 kg body weight). EPS was performed under a general anesthesia with midazolam, propofol and isoflurane. The reference values for intracardiac electrical activity and conduction parameters were calculated as arithmetic means ± 2 standard deviations. The reference values were determined for AH, HV and PA intervals, interatrial conduction time at its own and imposed rhythm, sinus node recovery time (SNRT), corrected sinus node recovery time (CSNRT), anterograde and retrograde Wenckebach points, atrial, atrioventricular node and ventricular refractory periods. No significant correlations were found between body weight and heart rate of the examined pigs and their electrophysiological parameters. The hereby presented reference values can be helpful in comparing the results of various studies, as well as in more accurately estimating the values of electrophysiological parameters that can be expected in a given experiment.

  1. A normalized wave number variation parameter for acoustic black hole design.

    Science.gov (United States)

    Feurtado, Philip A; Conlon, Stephen C; Semperlotti, Fabio

    2014-08-01

    In recent years, the concept of the Acoustic Black Hole has been developed as an efficient passive, lightweight absorber of bending waves in plates and beams. Theory predicts greater absorption for a higher thickness taper power. However, a higher taper power also increases the violation of an underlying theory smoothness assumption. This paper explores the effects of high taper power on the reflection coefficient and spatial change in wave number and discusses the normalized wave number variation as a spatial design parameter for performance, assessment, and optimization.

  2. Low Density Lipoprotein and Non-Newtonian Oscillating Flow Biomechanical Parameters for Normal Human Aorta.

    Science.gov (United States)

    Soulis, Johannes V; Fytanidis, Dimitrios K; Lampri, Olga P; Giannoglou, George D

    2016-04-01

    The temporal variation of the hemodynamic mechanical parameters during cardiac pulse wave is considered as an important atherogenic factor. Applying non-Newtonian blood molecular viscosity simulation is crucial for hemodynamic analysis. Understanding low density lipoprotein (LDL) distribution in relation to flow parameters will possibly spot the prone to atherosclerosis aorta regions. The biomechanical parameters tested were averaged wall shear stress (AWSS), oscillatory shear index (OSI) and relative residence time (RRT) in relation to the LDL concentration. Four non-Newtonian molecular viscosity models and the Newtonian one were tested for the normal human aorta under oscillating flow. The analysis was performed via computational fluid dynamic. Tested viscosity blood flow models for the biomechanical parameters yield a consistent aorta pattern. High OSI and low AWSS develop at the concave aorta regions. This is most noticeable in downstream flow region of the left subclavian artery and at concave ascending aorta. Concave aorta regions exhibit high RRT and elevated LDL. For the concave aorta site, the peak LDL value is 35.0% higher than its entrance value. For the convex site, it is 18.0%. High LDL endothelium regions located at the aorta concave site are well predicted with high RRT. We are in favor of using the non-Newtonian power law model for analysis. It satisfactorily approximates the molecular viscosity, WSS, OSI, RRT and LDL distribution. Concave regions are mostly prone to atherosclerosis. The flow biomechanical factor RRT is a relatively useful tool for identifying the localization of the atheromatic plaques of the normal human aorta.

  3. Differences of Sagittal Lumbosacral Parameters between Patients with Lumbar Spondylolysis and Normal Adults.

    Science.gov (United States)

    Yin, Jin; Peng, Bao-Gan; Li, Yong-Chao; Zhang, Nai-Yang; Yang, Liang; Li, Duan-Ming

    2016-05-20

    Recent studies have suggested an association between elevated pelvic incidence (PI) and the development of lumbar spondylolysis. However, there is still lack of investigation for Han Chinese people concerning the normal range of spinopelvic parameters and relationship between abnormal sagittal parameters and lumbar diseases. The objective of the study was to investigate sagittal lumbosacral parameters of adult lumbar spondylolysis patients in Han Chinese population. A total of 52 adult patients with symptomatic lumbar spondylolysis treated in the General Hospital of Armed Police Force (Beijing, China) were identified as the spondylolysis group. All the 52 patients were divided into two subgroups, Subgroup A: 36 patients with simple lumbar spondylolysis, and Subgroup B: 16 patients with lumbar spondylolysis accompanying with mild lumbar spondylolisthesis (slip percentage spondylolysis group and the control group with independent-sample t- test. There were no statistically significant differences of all seven sagittal lumbosacral parameters between Subgroup A and Subgroup B. PI, PT, SS, and LL were higher (P spondylolysis group than those in the control group, but STA was lower (P spondylolysis group. Current study results suggest that increased PI and decreased STA may play important roles in the pathology of lumbar spondylolysis in Han Chinese population.

  4. Age-related changes of the diffusion tensor imaging parameters of the normal cervical spinal cord

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Kun, E-mail: medsciwangkun@126.com [Orthopedics Department, Changhai Hospital Affiliated to Second Military Medical University, Shanghai (China); Song, Qingxin; Zhang, Fan; Chen, Zhi; Hou, Canglong; Tang, Yixing [Orthopedics Department, Changhai Hospital Affiliated to Second Military Medical University, Shanghai (China); Chen, Shiyue [Radiology Department, Changhai Hospital Affiliated to Second Military Medical University, Shanghai (China); Hao, Qiang, E-mail: haoqiang@189.cn [Radiology Department, Changhai Hospital Affiliated to Second Military Medical University, Shanghai (China); Shen, Hongxing, E-mail: shenhxgk@126.com [Orthopedics Department, Changhai Hospital Affiliated to Second Military Medical University, Shanghai (China)

    2014-12-15

    Highlights: • It is essential to determine the DTI parameters in the whole CSC. • To analyze DTI parameters in all intervertebral space levels of the CSC. • To study the impact of age on these parameters in healthy Chinese subjects. • Provide better insights in factors that could bias the diagnosis of CSC pathologies. - Abstract: Background: The diffusion tensor imaging (DTI) parameters of the cervical spinal cord (CSC) changes with age. However, previous studies only examined specific CSC areas. Objectives: To analyze the DTI parameters in all intervertebral space levels of the whole normal CSC and to study the impact of age on these parameters in a Chinese population. Methods: Thirty-six healthy subjects aged 20–77 years were recruited. DTI parameters were calculated for gray matter (GM) and white matter (WM) funiculi in all the CSC intervertebral spaces (C1/2-C6/7). Age-related changes of DTI parameters were analyzed for the GM and WM funiculi. Results: Fractional anisotropy (FA) and mean diffusivity (MD) were lower in GM than in WM. MD and FA values were lower in the WM in the lower CSC compared with the upper CSC (all P < 0.05), but no difference was observed in GM. In ventral funiculi, MD increased with age, while FA decreased (all P < 0.001). In lateral and dorsal funiculi, MD and FA decreased with age (all P < 0.001). In GM, MD and FA decreased with age (all P < 0.001). Significant age-related changes were observed in FA and MD from GM and WM funiculi. FA was correlated with age in all funiculi (ventral: r = −0.733; lateral: r = −0.468; dorsal: r = −0.607; GM: r = −0.724; all P < 0.01). Conclusion: Important changes in MD and FA were observed with advancing age at all levels of CSC in Chinese patients. DTI parameters may be useful to assess CSC pathology, but the influence of age and segments need to be taken into account in diagnosis.

  5. Age-related changes of the diffusion tensor imaging parameters of the normal cervical spinal cord

    International Nuclear Information System (INIS)

    Wang, Kun; Song, Qingxin; Zhang, Fan; Chen, Zhi; Hou, Canglong; Tang, Yixing; Chen, Shiyue; Hao, Qiang; Shen, Hongxing

    2014-01-01

    Highlights: • It is essential to determine the DTI parameters in the whole CSC. • To analyze DTI parameters in all intervertebral space levels of the CSC. • To study the impact of age on these parameters in healthy Chinese subjects. • Provide better insights in factors that could bias the diagnosis of CSC pathologies. - Abstract: Background: The diffusion tensor imaging (DTI) parameters of the cervical spinal cord (CSC) changes with age. However, previous studies only examined specific CSC areas. Objectives: To analyze the DTI parameters in all intervertebral space levels of the whole normal CSC and to study the impact of age on these parameters in a Chinese population. Methods: Thirty-six healthy subjects aged 20–77 years were recruited. DTI parameters were calculated for gray matter (GM) and white matter (WM) funiculi in all the CSC intervertebral spaces (C1/2-C6/7). Age-related changes of DTI parameters were analyzed for the GM and WM funiculi. Results: Fractional anisotropy (FA) and mean diffusivity (MD) were lower in GM than in WM. MD and FA values were lower in the WM in the lower CSC compared with the upper CSC (all P < 0.05), but no difference was observed in GM. In ventral funiculi, MD increased with age, while FA decreased (all P < 0.001). In lateral and dorsal funiculi, MD and FA decreased with age (all P < 0.001). In GM, MD and FA decreased with age (all P < 0.001). Significant age-related changes were observed in FA and MD from GM and WM funiculi. FA was correlated with age in all funiculi (ventral: r = −0.733; lateral: r = −0.468; dorsal: r = −0.607; GM: r = −0.724; all P < 0.01). Conclusion: Important changes in MD and FA were observed with advancing age at all levels of CSC in Chinese patients. DTI parameters may be useful to assess CSC pathology, but the influence of age and segments need to be taken into account in diagnosis

  6. CONNECTION BETWEEN DYNAMICALLY DERIVED INITIAL MASS FUNCTION NORMALIZATION AND STELLAR POPULATION PARAMETERS

    Energy Technology Data Exchange (ETDEWEB)

    McDermid, Richard M. [Department of Physics and Astronomy, Macquarie University, Sydney NSW 2109 (Australia); Cappellari, Michele; Bayet, Estelle; Bureau, Martin; Davies, Roger L. [Sub-Department of Astrophysics, Department of Physics, University of Oxford, Denys Wilkinson Building, Keble Road, Oxford, OX1 3RH (United Kingdom); Alatalo, Katherine [Infrared Processing and Analysis Center, California Institute of Technology, Pasadena, CA 91125 (United States); Blitz, Leo [Department of Astronomy, Campbell Hall, University of California, Berkeley, CA 94720 (United States); Bois, Maxime [Observatoire de Paris, LERMA and CNRS, 61 Av. de l' Observatoire, F-75014 Paris (France); Bournaud, Frédéric; Duc, Pierre-Alain [Laboratoire AIM Paris-Saclay, CEA/IRFU/SAp- CNRS-Université Paris Diderot, F-91191 Gif-sur-Yvette Cedex (France); Crocker, Alison F. [Ritter Astrophysical Observatory, University of Toledo, Toledo, OH 43606 (United States); Davis, Timothy A.; De Zeeuw, P. T.; Emsellem, Eric; Kuntschner, Harald [European Southern Observatory, Karl-Schwarzschild-Str. 2, D-85748 Garching (Germany); Khochfar, Sadegh [Institute for Astronomy, University of Edinburgh, Royal Observatory, Edinburgh, EH9 3HJ (United Kingdom); Krajnović, Davor [Leibniz-Institut für Astrophysik Potsdam (AIP), An der Sternwarte 16, D-14482 Potsdam (Germany); Morganti, Raffaella; Oosterloo, Tom [Netherlands Institute for Radio Astronomy (ASTRON), Postbus 2, 7990 AA Dwingeloo (Netherlands); Naab, Thorsten, E-mail: richard.mcdermid@mq.edu.au [Max-Planck-Institut für Astrophysik, Karl-Schwarzschild-Str. 1, D-85741 Garching (Germany); and others

    2014-09-10

    We report on empirical trends between the dynamically determined stellar initial mass function (IMF) and stellar population properties for a complete, volume-limited sample of 260 early-type galaxies from the ATLAS{sup 3D} project. We study trends between our dynamically derived IMF normalization α{sub dyn} ≡ (M/L){sub stars}/(M/L){sub Salp} and absorption line strengths, and interpret these via single stellar population-equivalent ages, abundance ratios (measured as [α/Fe]), and total metallicity, [Z/H]. We find that old and alpha-enhanced galaxies tend to have on average heavier (Salpeter-like) mass normalization of the IMF, but stellar population does not appear to be a good predictor of the IMF, with a large range of α{sub dyn} at a given population parameter. As a result, we find weak α{sub dyn}-[α/Fe] and α{sub dyn} –Age correlations and no significant α{sub dyn} –[Z/H] correlation. The observed trends appear significantly weaker than those reported in studies that measure the IMF normalization via the low-mass star demographics inferred through stellar spectral analysis.

  7. Earth Orientation Parameters from VLBI and GNSS Combined at the Normal Equation Level

    Science.gov (United States)

    Richard, Jean-Yves; Lambert, Sébastien; Bizouard, Christian; Becker, Olivier

    2017-04-01

    Current reference series (C04) of the International Earth Rotation and Reference Systems Service (IERS) are produced by a weighted combination of Earth orientation parameters (EOP) time series built up by combination centers of each technique (VLBI, GNSS, Laser ranging, DORIS). In the future, we plan to produce EOP by a direct combination of the normal equation of the four techniques. We present an intermediate step of this project: a combination of VLBI and GNSS pre-reduced, constraint-free, normal equations with the DYNAMO geodetic analysis software package developed and maintained by the French GRGS (Groupe de Recherche en Géodésie Spatiale). The used normal equations are those produced separately by the IVS and IGS combination centers. Our series cover 2002-2016. The estimation strategy consists of fixing quasar coordinates to their optimal values given by the latest realization of the International Celestial Reference Frame (ICRF), and most of station coordinates to the ITRF 2014 except for stations undergoing strong nonlinear displacements caused by, e.g., postseismic relaxation. These station coordinates are estimated as time series. The resulting EOP series are compared to intra-technique combinations and to the IERS-C04 reference series.

  8. Comparsion of Speech Breathing Parameters between Chemical Injured Patients Due to Mustard Gas and Normal Individuals

    Directory of Open Access Journals (Sweden)

    Fatemeh Heidari

    2008-04-01

    Full Text Available Objective: The respiration role is not limited to vital function but it also important in speech process. The present research is aimed to measure different parameters of speech breathing values in patients suffering Mustard Gas injuries versus normal individual. Materials & Methods: Research was applied as case-control, cross – sectional and analytical method. The sampling pattern was convenience. Among patients, 19 were selected within injured patients from Baghiatallah hospital and 20 from those of normal individuals from welfare and rehabilitation sciences university stuffs. Normal people were matched based on their age, gender and other criterias within 30-50 years of age. ST1 Dysphonia manufactured by G.M. Instruments Ltd. was used to measure parameters of speech breathing, that include “Vital capacity”, “Phonation time”,” Phonation volume”,” Mean flow rate”,” Total expired volume”. And also,” Vocal velocity index” and “Phonation Quotient” were calculated. The independent T-test was used for data analysis.  Results: Research findings shown statistically significant difference between two groups in Vital capacity (P<0/001, Phonation time (P<0/001, Phonation volume (P<0/001, Vocal velocity index (P=0/004, Total expired volume (P<0/001 and Phonation Quotient (P=0/046, but there was no significant difference in Mean flow rate between case and control group (P=0/615.  Conclusion: present research has indicated, chemical war substance, not only has rendering effects upon vital respiration, but also has alternate patient’s speech breathing capabilities greatly. Such finding can be measured and evaluated the disturbed phonatory criteria in such patients and be a valued guide for speech & language phatologists, during treating such patients.

  9. Reproducibility of CT Perfusion Parameters in Liver Tumors and Normal Liver

    Science.gov (United States)

    Ng, Chaan S.; Chandler, Adam G.; Wei, Wei; Herron, Delise H.; Anderson, Ella F.; Kurzrock, Razelle; Charnsangavej, Chusilp

    2011-01-01

    Purpose: To assess the reproducibility of computed tomographic (CT) perfusion measurements in liver tumors and normal liver and effects of motion and data acquisition time on parameters. Materials and Methods: Institutional review board approval and written informed consent were obtained for this prospective study. The study complied with HIPAA regulations. Two CT perfusion scans were obtained 2–7 days apart in seven patients with liver tumors with two scanning phases (phase 1: 30-second breath-hold cine; phase 2: six intermittent free-breathing cines) spanning 135 seconds. Blood flow (BF), blood volume (BV), mean transit time (MTT), and permeability–surface area product (PS) for tumors and normal liver were calculated from phase 1 with and without rigid registration and, for combined phases 1 and 2, with manually and rigid-registered phase 2 images, by using deconvolution modeling. Variability was assessed with within-patient coefficients of variation (CVs) and Bland-Altman analyses. Results: For tumors, BF, BV, MTT, and PS values and reproducibility varied by analytical method, the former by up to 11%, 23%, 21%, and 138%, respectively. Median PS values doubled with the addition of phase 2 data to phase 1 data. The best overall reproducibility was obtained with rigidly registered phase 1 and phase 2 images, with within-patient CVs for BF, BV, MTT, and PS of 11.2%, 14.4%, 5.5% and 12.1%, respectively. Normal liver evaluations were similar, except with marginally lower variability. Conclusion: Absolute values and reproducibility of CT perfusion parameters were markedly influenced by motion and data acquisition time. PS, in particular, probably requires data acquisition beyond a single breath hold, for which motion-correction techniques are likely necessary. © RSNA, 2011 Supplemental material: http://radiology.rsna.org/lookup/suppl/doi:10.1148/radiol.11110331/-/DC1 PMID:21788525

  10. The change of anterior segment parameters after cataract surgery in normal-tension glaucoma

    Directory of Open Access Journals (Sweden)

    Wonseok Lee

    2017-08-01

    Full Text Available AIM: To investigate the change of anterior chamber angle morphology and intraocular pressure (IOP reduction after cataract surgery in patients with normal-tension glaucoma (NTG using swept-source optical coherence tomography (SS-OCT. METHODS: This prospective, comparative, observational study recruited patients into two groups. Group 1 was the control group including normal subjects except those with cataracts (cataract group, n=67 eyes of 67 patients, and group 2 was NTG group including patients who were diagnosed with NTG and cataracts (n=43 eyes of 43 patients, which were treated with phacoemulsification and intraocular lens implantation. Before surgery, and at postoperative 1 and 6mo, anterior chamber angles were evaluated by SS-OCT under dark conditions using three-dimensional angle analysis scan protocol. Angle opening distance (AOD, angle recess area (ARA, and trabecular-iris surface area (TISA at four quadrants (temporal, nasal, superior, and inferior were calculated automatically by SS-OCT, after the observer marked scleral spurs. RESULTS: A total of 106 patients (54 males and 52 females were enrolled in the study. Angle parameters, AOD, ARA, and TISA were increased after surgery in both groups. However, changes of angle parameters were only significant in group 2. In group 2, preoperative IOP was 13.2±2.9 mm Hg, and postoperative IOP at 1 and 6mo were 10.5±3.0 and 10.7±2.8 mm Hg, respectively. In group 1, preoperative IOP was 12.4±2.8 mm Hg, and postoperative IOP at 1 and 6mo were 11.6±2.5 and 12.0±2.8 mm Hg, respectively. After cataract surgery, angle parameters changed significantly while IOP significantly reduced and was maintained in group 2 (P<0.001. The changes in angle parameters (ΔAOD500, ΔTISA500 at temporal; ΔAOD500, ΔARA500 at nasal were linearly correlated with postoperative IOP changes. CONCLUSION: Cataract surgery may have improved anterior chamber angle parameters and decreased IOP in NTG patients.

  11. Effect of graded levels of dietary seaweed (Ulva sp.) on growth, hematological and serum biochemical parameters in dusky kob, Argyrosomus japonicus, sciaenidae

    OpenAIRE

    Molatelo Junior Madibana; Victor Mlambo; Brett Lewis; Chris Fouché

    2017-01-01

    This study was designed to assess the effect of incorporating graded levels of green macroalgae seaweed (Ulva sp.) into diets of juvenile dusky kob (Argyrosomus japonicus) on growth performance, hematology and serum biochemistry. Five experimental diets were formulated to contain 0 (Ulva0), 50 (Ulva50), 100 (Ulva100), 150 (Ulva150) and 200 (Ulva200) g seaweed meal/kg commercial kob feed on a dry matter basis. Seventy-seven dusky kob fingerlings (mean body mass 9.14â¯Â±â¯0.30â¯g) were distribu...

  12. Hemoglobin variants, hematological parameters and beta-globin gene cluster haplotypes in an isolated Amerindian group from the Orinoco River Delta.

    Science.gov (United States)

    Arends, Anabel; Chacín, Marycarmen; Bravo-Urquiola, Martha; Tibisay, Arends De O; Alvarez, Maritza; Castillo, Omar; Guevara, Jose M

    2008-01-01

    Several previous studies reported that the Venezuelan Warao Indians presented unusual genetic characteristics. The present study checked previous reports of a high frequency of hereditary persistence of fetal hemoglobin (HPFH) and examined other hematological traits. Standard hematology, electrophoresis on cellulose acetate, fetal hemoglobin alkali denaturation, gamma-globin chain, DNA amplification and sequencing, and denaturing gradient gel electrophoresis determinations were performed in 269 individuals living in two localities of the Orinoco River Delta. Two beta(s) genes, in apparently non-related individuals, were found. HPFH, detected in this same population of Warao Indians 25 years ago, was present in heterozygous form in five individuals from a large kindred, with hemoglobin F levels ranging from 3.7% to 8%, and with a pancellular distribution. The HPFH mutation was of the deletional type. beta-globin gene haplotypes were determined by direct counting (through family studies) in 150 chromosomes; 26% of the 150 examined cluster presented haplotype 2, 22% haplotype 6, and 13% a new, Warao haplotype. Haplotype 3, of probable African origin, was also found with a frequency of 5%. The presence of the HPFH mutation was confirmed, and the new beta-globin gene haplotype together with the presence of other rare variants indicates that the Warao are very distinctive in relation to other Native Americans. Evidence was also found of a slight admixture from Africa-derived subjects (Layrisse et al. 1988).

  13. Survey of Saccadic Parameters Using Videonystagmography in Patients with Idiopathic Parkinson's Disease and Normal Subjects

    Directory of Open Access Journals (Sweden)

    Reza Hosseinabadi

    2008-06-01

    Full Text Available Background and Aim: Patients with Parkinson’s disease manifest oculomotor abnormalities. This is the consequence of basal ganglia impairment. The most common abnormalities include increased saccade latency, hypometric saccades and decreased saccade velocity. The purpose of this study was comparison of saccadic parameters using videonystagmography in patients with idiopathic Parkinson’s disease and normal subjects.Materials and Methods: In this cross sectional study, saccadic movements were investigated in thirty patients with idiopathic Parkinson’s disease and thirty age matched subjects were 35-70 years old. Saccade latency, velocity and accuracy were quantitatively analyzed. Results: Results of this study indicated increased saccade latency, reduction of saccade velocity and accuracy in patients with Parkinson’s disease(P<0.001.Conclusion: This study showed that patients with Parkinson’s disease manifest saccadic deficits. This suggests dopaminergic control of these ocular movements.

  14. Parameters of phosphorus homeostasis at normal and reduced GFR: empiric observations.

    Science.gov (United States)

    Phelps, Kenneth R; Mason, Darius L; Stote, Kim S

    2015-04-01

    Influx (IP) determines urinary excretion of phosphorus (EP). Contributions of IP and reabsorption (TRP) to serum phosphorus ([P]s) can be depicted by normalization to creatinine clearance (EP/Ccr and TRP/Ccr) or by calculation of fractional excretion and reabsorption (FEP and FTRP). We analyzed these parameters at normal and reduced GFR. We studied 29 patients with chronic kidney disease (CKD) and 28 controls. From [cr] and [P] in serumand urine we calculated [P]u/[cr]u, EP/Ccr, TRP/Ccr, (TRP/Ccr)/(EP/Ccr), FEP, and FTRP. We compared means between groups and examined pertinent linear regressions. [P]s was not different in CKD and controls. [Cr]s, EP/Ccr, and FEP were higher and TRP/Ccr, (TRP/Ccr)/(EP/Ccr), and FTRP were lower in CKD. [P]u/[cr]u, a surrogate for IP, was similar in both groups. In CKD, [P]s correlated with EP/Ccr and TRP/Ccr; EP/Ccr with [P]u/[cr]u and [cr]s; and FEP with EP/Ccr, TRP/Ccr, [P]u/[cr]u, and [cr]s. In controls, [P]s correlated with TRP/Ccr; EP/Ccr with [P]u/[cr]u; and FEP with EP/Ccr and [P]u/[cr]u. In both groups, FEP was a precise inverse function of (TRP/Ccr)/(EP/Ccr). Despite wide variation in TRP/Ccr, FEP was 20% in 27/29 patients with CKD. GFR affected determinants of [P]s, EP/Ccr, and FEP. FTRP was often dissociated from TRP/Ccr at normal or reduced GFR.

  15. A Novel Heme Pocket Hemoglobin Variant Associated with Normal Hematology: Hb Zara or α91(FG3)Leu→Ile (α2) (HBA2: c.274C > A).

    Science.gov (United States)

    Trova, Sandro; Mereu, Paolo; Decandia, Luca; Cocco, Elena; Masala, Bruno; Manca, Laura; Pirastru, Monica

    2015-08-13

    We report a new hemoglobin (Hb) variant on the HBA2 gene, Hb Zara [α91(FG3)Leu→Ile (α2); HBA2: c.274C > A], which was found in a Caucasian man from Croatia. It was observed by routine cation exchange chromatography as an abnormal 21.8% fraction overlapping Hb A 2 , and associated with normal hematology. It was slightly unstable by the standard isopropanol precipitation test. DNA analysis revealed the CTT > ATT mutation at codon 91 on an α2 gene of a normal α-globin gene arrangement. This new variant represents the sixth described mutation at codon α91 and fourth on the α2 locus. As a result of the slight instability due to the significant role of the α91 residue in the α1β2 contact, the level of the Hb Zara variant was lower than levels observed for several stable variants codified by the α2 locus.

  16. [Experimental infection of the mule duck by Eimeria mulardi sp. nov.: effects on body weight and modifications of different hematologic and biochemical parameters].

    Science.gov (United States)

    Pascalon-Pekelniczky, A; Chauve, C M; Gauthey, M

    1994-01-01

    Eleven-day-old female Mule ducks (Cairina moschata x Anas platyrhynchos) were individually infected per os with a single inoculation containing 10(4), 10(5) or 10(6) sporulated oocysts of E mulardi, or kept as uninfected controls. No mortality was observed. Growth rate was not modified at lower doses, but was reduced in the other 2 groups, with weight loss on d 7 post inoculation (PI). Blood was detected in feces by chemical characterization of hemoglobin as early as d 3 PI with the highest infecting dose, while oocyst excretion began on d 6 PI in all infected groups. With 10(5) and 10(6) oocysts, the authors observed a significant increase in sedimentation rate and a slight anemia. Total protein and lipid concentrations in the plasma decreased in the 3 infected groups. These results show the pathogenic effect of E mulardi in Mule duckling, with dose-dependent changes. All parameters, except sedimentation rate, showed maximal variations during the acute phase of infection, between d 6 and d 9 PI, and returned to normal values early, in relation to the infecting dose, except that the weight remained lower than in the uninfected group at the final control (d 28 Pl). These perturbations have been described in avian coccidiosis.

  17. Morbillivirus and Toxoplasma exposure and association with hematological parameters for southern Beaufort Sea polar bears: potential response to infectious agents in a sentinel species.

    Science.gov (United States)

    Kirk, Cassandra M; Amstrup, Steven; Swor, Rhonda; Holcomb, Darce; O'Hara, Todd M

    2010-09-01

    Arctic temperatures are increasing in response to greenhouse gas forcing and polar bears have already responded to changing conditions. Declines in body stature and vital rates have been linked to warming-induced loss of sea-ice. As food webs change and human activities respond to a milder Arctic, exposure of polar bears and other arctic marine organisms to infectious agents may increase. Because of the polar bear's status as arctic ecosystem sentinel, polar bear health could provide an index of changing pathogen occurrence throughout the Arctic, however, exposure and monitoring protocols have yet to be established. We examine prevalence of antibodies to Toxoplasma gondii, and four morbilliviruses (canine distemper [CDV], phocine distemper [PDV], dolphin morbillivirus [DMV], porpoise morbillivirus [PMV]) including risk factors for exposure. We also examine the relationships between antibody levels and hematologic values established in the previous companion article. Antibodies to Toxoplasma gondii and morbilliviruses were found in both sample years. We found a significant inverse relationship between CDV titer and total leukocytes, neutrophils, monocytes, and eosinophils, and a significant positive relationship between eosinophils and Toxoplasma gondii antibodies. Morbilliviral prevalence varied significantly among age cohorts, with 1-2 year olds least likely to be seropositive and bears aged 5-7 most likely. Data suggest that the presence of CDV and Toxoplasma gondii antibodies is associated with polar bear hematologic values. We conclude that exposure to CDV-like antigen is not randomly distributed among age classes and suggest that differing behaviors among life history stages may drive probability of specific antibody presence.

  18. Morbillivirus and Toxoplasma exposure and association with hematological parameters for southern Beaufort Sea polar bears: potential response to infectious agents in a sentinel species

    Science.gov (United States)

    Kirk, Cassandra M.; Amstrup, Steven C.; Swor, Rhonda; Holcomb, Darce; O'Hara, Todd M.

    2010-01-01

    Arctic temperatures are increasing in response to greenhouse gas forcing and polar bears have already responded to changing conditions. Declines in body stature and vital rates have been linked to warming-induced loss of sea-ice. As food webs change and human activities respond to a milder Arctic, exposure of polar bears and other arctic marine organisms to infectious agents may increase. Because of the polar bear’s status as arctic ecosystem sentinel, polar bear health could provide an index of changing pathogen occurrence throughout the Arctic, however, exposure and monitoring protocols have yet to be established. We examine prevalence of antibodies to Toxoplasma gondii, and four morbilliviruses (canine distemper [CDV], phocine distemper [PDV], dolphin morbillivirus [DMV], porpoise morbillivirus [PMV]) including risk factors for exposure. We also examine the relationships between antibody levels and hematologic values established in the previous companion article. Antibodies to Toxoplasma gondii and morbilliviruses were found in both sample years. We found a significant inverse relationship between CDV titer and total leukocytes, neutrophils, monocytes, and eosinophils, and a significant positive relationship between eosinophils and Toxoplasma gondii antibodies. Morbilliviral prevalence varied significantly among age cohorts, with 1–2 year olds least likely to be seropositive and bears aged 5–7 most likely. Data suggest that the presence of CDV and Toxoplasma gondii antibodies is associated with polar bear hematologic values. We conclude that exposure to CDV-like antigen is not randomly distributed among age classes and suggest that differing behaviors among life history stages may drive probability of specific antibody presence.

  19. Effect of graded levels of dietary seaweed (Ulva sp. on growth, hematological and serum biochemical parameters in dusky kob, Argyrosomus japonicus, sciaenidae

    Directory of Open Access Journals (Sweden)

    Molatelo Junior Madibana

    2017-09-01

    Full Text Available This study was designed to assess the effect of incorporating graded levels of green macroalgae seaweed (Ulva sp. into diets of juvenile dusky kob (Argyrosomus japonicus on growth performance, hematology and serum biochemistry. Five experimental diets were formulated to contain 0 (Ulva0, 50 (Ulva50, 100 (Ulva100, 150 (Ulva150 and 200 (Ulva200 g seaweed meal/kg commercial kob feed on a dry matter basis. Seventy-seven dusky kob fingerlings (mean body mass 9.14 ± 0.30 g were distributed into each of 20 experimental tanks and offered diets at 2.8% body mass. Weight and length of fish were measured weekly for 9 weeks. At the end of the feeding trial, fish fed Ulva50 had higher body mass (38.20 ± 1.38 g compared to those fed the other three Ulva-based diets. Fish offered Ulva200 had significantly (P  .05 by dietary treatments. However, the levels of both blood cholesterol and alkaline phosphatase tended to decrease as dietary Ulva inclusion levels increased. These results indicate that Ulva could be incorporated at the rate of 50 g kg−1 in future commercial dusky kob diets without any negative effects on feed utilizationa and growth performance. Hematology and serum biochemistry results indicate that dietary inclusion of Ulva had no adverse effects on physiological status of the fish. Keywords: Ulva sp., Haematology, Dusky kob, Feed conversion efficiency, Specific growth rate

  20. Conditional Frequentist Test on Normal Mean Parameter and its application in entomological data

    Directory of Open Access Journals (Sweden)

    Carla R. G. Brighenti

    2014-10-01

    Full Text Available In the traditional tests the value of type I error (α is fixed, on the other hand, in the conditional frequentist test we propose a partition of the region of rejection and acceptance so that the probability of error presented is dependent on the distance of observed data in relation to the border of the critical region. This error probability is the Conditional Error Probability (CEP, which may be of type I or II. The objective of this study was to evaluate the behavior of the conditional frequentist test under the Normal Mean parameter and apply it to entomological data. The test was evaluated via simulation, from developed routine in R software. In each simulated case obtained the likelihood ratio, the critical value and the PEC’s for the Normal distribution. It was concluded that the highest values of PEC were obtained with the more the sample mean value approaching the critical point of the test. This occurs regardless of the decision of the test to accept or reject the null hypothesis. The increase of the sample size causes the reduction of the conditional probability of error. In the application to real data from the average lifetime of bees exposed to different temperatures, it was found that the average lifetime of bees maintained at a temperature of 20°C was found to be similar at a temperature of 15°C with conditional error very small (CEP II equal to 0.54%. For temperatures of 30°C and 35°C the hypothesis was rejected with PEC I equal to 28.35% and 47.53%, respectively. Thus the conditional errors in decision making were very high.

  1. A Review on hematology and hemoglobin of fish

    Directory of Open Access Journals (Sweden)

    Ebru YILMAZ

    2015-01-01

    Full Text Available Determination of hematological parameters of fish living in natüre helps to recognize population and to determinate of pollutants in the aquatic environment. In this review, hematological parameters of fish, fish hemoglobin and the Bohr effect were given information.

  2. Hematological and enzymatic responses of Nile tilapia Oreochromis ...

    African Journals Online (AJOL)

    Results of the present study suggest that sublethal concentrations of zinc affect the hematological changes and impaired liver functions. These parameters can be useful in environmental biomonitoring of zinc contamination. Key words: Nile tilapia, Oreochromis niloticus, liver enzymes, hematological parameters, LCa50 of ...

  3. Optimization of accelerator parameters using normal form methods on high-order transfer maps

    Energy Technology Data Exchange (ETDEWEB)

    Snopok, Pavel [Michigan State Univ., East Lansing, MI (United States)

    2007-05-01

    Methods of analysis of the dynamics of ensembles of charged particles in collider rings are developed. The following problems are posed and solved using normal form transformations and other methods of perturbative nonlinear dynamics: (1) Optimization of the Tevatron dynamics: (a) Skew quadrupole correction of the dynamics of particles in the Tevatron in the presence of the systematic skew quadrupole errors in dipoles; (b) Calculation of the nonlinear tune shift with amplitude based on the results of measurements and the linear lattice information; (2) Optimization of the Muon Collider storage ring: (a) Computation and optimization of the dynamic aperture of the Muon Collider 50 x 50 GeV storage ring using higher order correctors; (b) 750 x 750 GeV Muon Collider storage ring lattice design matching the Tevatron footprint. The normal form coordinates have a very important advantage over the particle optical coordinates: if the transformation can be carried out successfully (general restrictions for that are not much stronger than the typical restrictions imposed on the behavior of the particles in the accelerator) then the motion in the new coordinates has a very clean representation allowing to extract more information about the dynamics of particles, and they are very convenient for the purposes of visualization. All the problem formulations include the derivation of the objective functions, which are later used in the optimization process using various optimization algorithms. Algorithms used to solve the problems are specific to collider rings, and applicable to similar problems arising on other machines of the same type. The details of the long-term behavior of the systems are studied to ensure the their stability for the desired number of turns. The algorithm of the normal form transformation is of great value for such problems as it gives much extra information about the disturbing factors. In addition to the fact that the dynamics of particles is represented

  4. Effects of dietary protein content on renal parameters in normal cats.

    Science.gov (United States)

    Backlund, Brianna; Zoran, Debra L; Nabity, Mary B; Norby, Bo; Bauer, John E

    2011-10-01

    This study evaluates the effect of dietary protein content on renal parameters in 23 healthy spayed female cats. The objective was to determine if cats eating diets high in protein will have higher serum urea nitrogen (UN) and creatinine values without a detectable change in kidney function, as assessed by urinalysis. A single random cross-over design was used. Cats were fed a standard maintenance diet for at least 1 month prior to the dietary trial. They were fed in two phases. For the first phase, cats were randomly assigned to receive either a high protein [HP=46% metabolizable energy (ME)] or low protein (LP=26% ME) diet. For the second phase, cats were fed whichever diet they were not fed during the phase I period. Blood and urine samples were collected at 2-week intervals for the duration of the study (10 weeks). UN, albumin, alanine aminotransferase and urine specific gravity were significantly higher, and creatinine and phosphorus were significantly lower (P<0.05) when cats were fed the HP diet as compared to when they were fed the LP diet, although none of the mean values were found to be outside of the corresponding reference interval. Dietary intake can result in clinically significant changes in UN and statistically significantly changes in several other biochemical analytes, although all analytes are likely to remain within normal reference intervals. Therefore, an accurate dietary history is necessary to help determine if renal parameters are being influenced by diet in a particular patient. Copyright © 2011 ISFM and AAFP. Published by Elsevier Ltd. All rights reserved.

  5. An iterative procedure for obtaining maximum-likelihood estimates of the parameters for a mixture of normal distributions, Addendum

    Science.gov (United States)

    Peters, B. C., Jr.; Walker, H. F.

    1975-01-01

    New results and insights concerning a previously published iterative procedure for obtaining maximum-likelihood estimates of the parameters for a mixture of normal distributions were discussed. It was shown that the procedure converges locally to the consistent maximum likelihood estimate as long as a specified parameter is bounded between two limits. Bound values were given to yield optimal local convergence.

  6. Effect of Nebivolol on MIBG Parameters and Exercise in Heart Failure with Normal Ejection Fraction

    Energy Technology Data Exchange (ETDEWEB)

    Messias, Leandro Rocha, E-mail: lmessias@cardiol.br; Ferreira, Aryanne Guimarães; Miranda, Sandra Marina Ribeiro de; Teixeira, José Antônio Caldas [Universidade Federal Fluminense, Rio de Janeiro, RJ (Brazil); Azevedo, Jader Cunha de [Universidade Federal Fluminense, Rio de Janeiro, RJ (Brazil); Hospital Procardíaco, Rio de Janeiro, RJ (Brazil); Messias, Ana Carolina Nader Vasconcelos [Hospital Federal dos Servidores do Estado do Rio de Janeiro, Rio de Janeiro, RJ (Brazil); Maróstica, Elisabeth [Universidade Federal Fluminense, Rio de Janeiro, RJ (Brazil); Mesquita, Claudio Tinoco [Universidade Federal Fluminense, Rio de Janeiro, RJ (Brazil); Hospital Procardíaco, Rio de Janeiro, RJ (Brazil)

    2016-05-15

    More than 50% of the patients with heart failure have normal ejection fraction (HFNEF). Iodine-123 metaiodobenzylguanidine (123I-MIBG) scintigraphy and cardiopulmonary exercise test (CPET) are prognostic markers in HFNEF. Nebivolol is a beta-blocker with vasodilating properties. To evaluate the impact of nebivolol therapy on CPET and123I-MIBG scintigraphic parameters in patients with HFNEF. Twenty-five patients underwent 123I-MIBG scintigraphy to determine the washout rate and early and late heart-to-mediastinum ratios. During the CPET, we analyzed the systolic blood pressure (SBP) response, heart rate (HR) during effort and recovery (HRR), and oxygen uptake (VO{sub 2}). After the initial evaluation, we divided our cohort into control and intervention groups. We then started nebivolol and repeated the tests after 3 months. After treatment, the intervention group showed improvement in rest SBP (149 mmHg [143.5-171 mmHg] versus 135 mmHg [125-151 mmHg, p = 0.016]), rest HR (78 bpm [65.5-84 bpm] versus 64.5 bpm [57.5-75.5 bpm, p = 0.028]), peak SBP (235 mmHg [216.5-249 mmHg] versus 198 mmHg [191-220.5 mmHg], p = 0.001), peak HR (124.5 bpm [115-142 bpm] versus 115 bpm [103.7-124 bpm], p= 0.043), HRR on the 1st minute (6.5 bpm [4.75-12.75 bpm] versus 14.5 bpm [6.7-22 bpm], p = 0.025) and HRR on the 2nd minute (15.5 bpm [13-21.75 bpm] versus 23.5 bpm [16-31.7 bpm], p = 0.005), but no change in peak VO{sub 2} and 123I-MIBG scintigraphic parameters. Despite a better control in SBP, HR during rest and exercise, and improvement in HRR, nebivolol failed to show a positive effect on peak VO2 and 123I-MIBG scintigraphic parameters. The lack of effect on adrenergic activity may be the cause of the lack of effect on functional capacity.

  7. Effect of Nebivolol on MIBG Parameters and Exercise in Heart Failure with Normal Ejection Fraction

    Directory of Open Access Journals (Sweden)

    Leandro Rocha Messias

    2016-01-01

    Full Text Available Abstract Background: More than 50% of the patients with heart failure have normal ejection fraction (HFNEF. Iodine-123 metaiodobenzylguanidine (123I-MIBG scintigraphy and cardiopulmonary exercise test (CPET are prognostic markers in HFNEF. Nebivolol is a beta-blocker with vasodilating properties. Objectives: To evaluate the impact of nebivolol therapy on CPET and123I-MIBG scintigraphic parameters in patients with HFNEF. Methods: Twenty-five patients underwent 123I-MIBG scintigraphy to determine the washout rate and early and late heart-to-mediastinum ratios. During the CPET, we analyzed the systolic blood pressure (SBP response, heart rate (HR during effort and recovery (HRR, and oxygen uptake (VO2. After the initial evaluation, we divided our cohort into control and intervention groups. We then started nebivolol and repeated the tests after 3 months. Results: After treatment, the intervention group showed improvement in rest SBP (149 mmHg [143.5-171 mmHg] versus 135 mmHg [125-151 mmHg, p = 0.016], rest HR (78 bpm [65.5-84 bpm] versus 64.5 bpm [57.5-75.5 bpm, p = 0.028], peak SBP (235 mmHg [216.5-249 mmHg] versus 198 mmHg [191-220.5 mmHg], p = 0.001, peak HR (124.5 bpm [115-142 bpm] versus 115 bpm [103.7-124 bpm], p= 0.043, HRR on the 1st minute (6.5 bpm [4.75-12.75 bpm] versus 14.5 bpm [6.7-22 bpm], p = 0.025 and HRR on the 2nd minute (15.5 bpm [13-21.75 bpm] versus 23.5 bpm [16-31.7 bpm], p = 0.005, but no change in peak VO2 and 123I-MIBG scintigraphic parameters. Conclusion: Despite a better control in SBP, HR during rest and exercise, and improvement in HRR, nebivolol failed to show a positive effect on peak VO2 and 123I-MIBG scintigraphic parameters. The lack of effect on adrenergic activity may be the cause of the lack of effect on functional capacity.

  8. Hematologic Disorders: Anemia.

    Science.gov (United States)

    Baltierra, David; Harper, Tiffany; Jones, Matthew Page; Nau, Konrad C

    2015-06-01

    Anemia occurs in up to 25% of the US population. Normal hemoglobin levels vary by race, sex, and age. Classification of anemia by mean corpuscular volume guides the differential diagnosis and evaluation. Iron studies, reticulocyte count, the red blood cell distribution width index, and blood test results are used to make the diagnosis. Iron deficiency anemia is the most common microcytic anemia and is managed with iron therapy. Parenteral iron is available when the oral route cannot be used. Patients who do not benefit from therapy should be evaluated for adherence, malabsorption, occult bleeding, systemic disease, or less common inherited disorders. A source of gastrointestinal bleeding is found in 60% to 70% of patients with iron deficiency anemia who are referred for endoscopy. Normocytic anemia has a broad differential, including nutritional deficiencies, blood loss, renal disease, malignancy (solid tumors or hematologic cancer), rheumatologic disorders, endocrine disorders, and other systemic diseases. Macrocytic anemias are seen with vitamin B12 and folate deficiency, alcohol use, thyroid disease, hydroxyurea, antiretroviral drugs, myelodysplastic syndromes, and myeloma. Oral vitamin B12 is underused, and can be as effective as intramuscular vitamin B12 in managing anemia due to vitamin B12 deficiency. Written permission from the American Academy of Family Physicians is required for reproduction of this material in whole or in part in any form or medium.

  9. Biochemical and hematological changes among saudi firefighters in the eastern province

    Directory of Open Access Journals (Sweden)

    Khaled Fikry Salama

    2017-01-01

    Full Text Available Aims: This study aimed to evaluate some relevant serum biochemical and hematological changes in concentration level involved firefighters in comparison to normal controls. Materials and Methods: This study involved two groups of male firefighters to participate in the study. The first group included 50 firefighters from Dammam while the second group included 50 firefighters from Khobar. An additional control group of 50 male nonfirefighters from both cities was included in the study. Blood samples were collected from all participants and investigated for some relevant biochemical, hematological differences and blood heavy metals between the three studied groups. Results: The results obtained showed that there were statistically significant differences in liver function, serum lipid profile, creatine kinase, lactate dehydrogenase, iron and ferritin, and blood picture in firefighters as compared with normal control group, while there were no statistically significant differences in the levels of blood heavy metals in firefighters as compared to normal controls. These results indicate that fire smoke mainly affects serum biochemical and hematological parameters but does not affect serum heavy metals levels. Conclusion: Such results might point out to the need for more health protective and prophylactic measures to try to avoid such hazardous health effects that might endanger firefighters under their highly drastic working conditions, and firefighters must be under continuous medical follow-up through a standard timetabled medical laboratory investigations to allow for the early detection of any biochemical or hematological changes.

  10. Minimal residual disease and normalization of the bone marrow after long-term treatment with alpha-interferon2b in polycythemia vera. A report on molecular response patterns in seven patients in sustained complete hematological remission

    DEFF Research Database (Denmark)

    Larsen, Thomas Stauffer; Møller, Michael Boe; de Stricker, Karin

    2009-01-01

    PV patients with profound molecular responses during and after long-term treatment with alpha-interferon 2b. All patients obtained a major molecular response (MMR). Subsequently all patients discontinued alpha-interferon and sustained complete hematological remission with a follow-up period of median...... of histomorphological bone marrow features of PV. Finally, hematological remissions and major molecular responses can be sustained after discontinuation of long-term treatment with IFN2b....

  11. Hematological evaluation of splenomegaly

    International Nuclear Information System (INIS)

    Ali, N.; Anwar, M.; Ayyub, M.; Ejaz, A.; Nadeem, M.; Qureshi, A.H.; Qamar, M.A.

    2004-01-01

    Objective: To find out the relative frequency of clinical conditions associated with splenomegaly that require hematological evaluation in our set up. Subjects and Methods: Patients of either gender or all age groups with palpable spleen was included. Patients with splenomegaly due to liver disease, malarial parasites on thick or thin blood film, positive Widal test, or positive blood cultures were excluded from study. Patients were initially evaluated with clinical history, microscopic examination of blood smear, and blood counts. Depending upon provisional diagnosis bone marrow examination or investigations for hemolytic anemia were performed. Results: One hundred patients were received. Seventy-eight patients were adults and 22 patients were of pediatric age group. In the adults, hematological malignancies were seen in 37%, malarial parasites in bone marrow in 20.5%, megaloblastic anemia in 13%, bacterial infections in 9%, hemolytic anemia in 9%, tropical splenomegaly in 5%, and positive bone marrow culture for salmonella in 6.5%. In children, hematological evaluation revealed hematological malignancies in 18%, beta thalassaemia in 55%, other hemolytic anemias in 13.5%, congenital sideroblastic anemia in 4.5%, and storage disorder in 9%. Conclusion: Hematological workup is informative in most of the cases. Bone marrow examination is the key investigation, hematological malignancies constituted 37% of the adult and 18% of pediatric age group patients. Hemolytic anemia constituted 68% of pediatric age group. (author)

  12. Living at high altitude in combination with sea-level sprint training increases hematological parameters but does not improve performance in rats.

    Science.gov (United States)

    Martinez-Bello, Vladimir Essau; Sanchis-Gomar, Fabian; Nascimento, Ana Lucia; Pallardo, Federico V; Ibañez-Sania, Sandra; Olaso-Gonzalez, Gloria; Calbet, Jose Antonio; Gomez-Cabrera, Mari Carmen; Viña, Jose

    2011-06-01

    The regimen of aerobic training at sea level with recovery at high altitude has been used by athletes to improve performance. However, little is known about the effects of hypoxia when combined with sprint interval training on performance. The aim of the present study was to determine the effect of a "living high-sprint training low" strategy on hemoglobin, hematocrit and erythropoietin levels in rats. We also wanted to test whether the addition of a hypoxic stress to the program of daily treadmill running at high speeds induces expressional adaptations in skeletal muscle and affects performance. The protein content of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), cytochrome C, pyruvate dehydrogenase kinase (PDK1), heat shock protein 70 (HSP70), manganese superoxide dismutase (MnSOD) and citrate synthase activity were determined in different muscle fiber types in our animals (red and white gastrocnemius muscle). We also determined the maximal aerobic velocity (MAV) before and after the training period. A total of 24 male Wistar rats (3 month old) were randomly divided into four experimental groups: the normoxic control group (n = 6), the normoxic trained group (n = 6), the hypoxic control group (12 h pO(2) 12%/12 h pO(2) 21%) (n = 6) and the hypoxic trained group (12 h pO(2) 12%/12 h pO(2) 21%). Living in normobaric hypoxia condition for 21 days significantly increased hemoglobin, hematocrit and erythropoietin levels in both the rest and the trained groups. The trained animals (normoxia and hypoxia) significantly increased their maximal aerobic velocity. No changes were found in the skeletal muscle in PGC-1α, cytochrome C, PDK1, HSP70, MnSOD protein content and in the citrate synthase activity in any experimental group. Regardless of whether it is combined with sprint interval training or not, after 21 days of living at high altitude we found a significant increase in the hematological values determined in our study. However, contrary to

  13. Growth and hematologic characteristics of cloned dogs derived from adult somatic cell nuclear transfer.

    Science.gov (United States)

    Park, Jung Eun; Kim, Min Kyu; Kang, Jung Taek; Oh, Hyun Ju; Hong, So Gun; Kim, Dae Young; Jang, Goo; Lee, Byeong Chun

    2010-04-01

    Three viable female dogs, which have the same genotype, have been successfully produced by somatic cell nuclear transfer (SCNT); however, data on the growth pattern of cloned dogs are lacking. Thus, the aim of this study was (1) to assess growth parameters among those cloned dogs with measurement of body weight, height, and radiographic analysis of skull size and bone plate, and (2) to compare hematologic characteristics among the donor dog, cloned dogs, and age-matched control dogs. The cloned dogs were kept in the same environmental conditions. The body weight increased from 0.52, 0.46, and 0.52 kg at birth to 21.9, 22.9, and 20.4 kg at 68 weeks of age for individual cloned dogs, respectively. The withers height increased from 34.5, 32.6, and 35.2 cm at 8 weeks of age to 67.1 cm at 68 weeks of age in the three clones. The radiographic data demonstrated that patterns of bone growth were similar among cloned dogs, and all measured parameters of matured cloned dogs were similar with that of the fully grown donor dog. An age-specific pattern was identified on hematologic and serum biochemical measurements in both cloned dogs and age-matched controls. The parameters examined were within the normal reference ranges for healthy dogs. In conclusion, three genetically identical cloned dogs showed similar growth characteristics and had normal hematological and serum biochemical parameters.

  14. The influence of joint parameters on normal fault evolution and geometry: a parameter study using analogue modeling

    Science.gov (United States)

    Kettermann, Michael; von Hagke, Christoph; Urai, Janos L.

    2017-04-01

    Dilatant faults often form in rocks containing pre-existing joints, but the effects of joints on fault segment linkage and fracture connectivity is not well understood. Studying evolution of dilatancy and influence of fractures on fault development provides insights into geometry of fault zones in brittle rocks and will eventually allow for predicting their subsurface appearance. In an earlier study we recognized the effect of different angles between strike direction of vertical joints and a basement fault on the geometry of a developing fault zone. We now systematically extend the results by varying geometric joint parameters such as joint spacing and vertical extent of the joints and measuring fracture density and connectivity. A reproducibility study shows a small error-range for the measurements, allowing for a confident use of the experimental setup. Analogue models were carried out in a manually driven deformation box (30x28x20 cm) with a 60° dipping pre-defined basement fault and 4.5 cm of displacement. To produce open joints prior to faulting, sheets of paper were mounted in the box to a depth of 5 cm at a spacing of 2.5 cm. We varied the vertical extent of the joints from 5 to 50 mm. Powder was then sieved into the box, embedding the paper almost entirely (column height of 19 cm), and the paper was removed. During deformation we captured structural information by time-lapse photography that allows particle imaging velocimetry analyses (PIV) to detect localized deformation at every increment of displacement. Post-mortem photogrammetry preserves the final 3-dimensional structure of the fault zone. A counterintuitive result is that joint depth is of only minor importance for the evolution of the fault zone. Even very shallow joints form weak areas at which the fault starts to form and propagate. More important is joint spacing. Very large joint spacing leads to faults and secondary fractures that form subparallel to the basement fault. In contrast, small

  15. The Impact of XmnI-HBG2, BCL11A and HBS1L-MYB Single Nucleotide Polymorphisms on Hb F Variation of Hematologically Normal Iranian Individuals.

    Science.gov (United States)

    Keyhani, Elaheh; Jafari Vesiehsari, Mahjoobeh; Talebi Kakroodi, Setareh; Darabi, Elham; Zamani, Fahimeh; Karimlou, Masoud; Kamali, Koorosh; Neishabury, Maryam

    2016-06-01

    The impact of Hb F on severity of sickle cell disease and β-thalassemia (β-thal) is well documented. The XmnI-HBG2, BCL11A and HBS1L-MYB single nucleotide polymorphisms (SNPs) have been introduced as the most important factors causing variation in fetal hemoglobin (Hb F) levels in different population studies. However, the extent of their effect could be population-specific. In this study, multivariate linear regression analysis was used to evaluate the association of Hb F with age, sex, and eight SNPs, including XmnI-HBG2, four BCL11A, two HBS1L-MYB SNPs and the polymorphic palindromic 5' hypersensitive 4-locus control region (5'HS4-LCR). One hundred and twenty-two hematologically normal individuals, from a previous study cohort, constituted our study population. In multivariate regression analyses, no association of Hb F was observed with age or sex of the individuals and SNPs in this study. We conducted a univariate regression analysis to further investigate the results, which among all the factors only detected XmnI-HBG2 and 5'HS4 SNPs as significant modifiers of Hb F. The significance of these two factors disappeared in a bivariate analysis. These results suggest that either XmnI-HBG2 or 5'HS4-LCR have a stronger contribution in Hb F variations of the Iranian population than BCL11A and HBS1L-MYB SNPs. Furthermore, the effect of low population size and technical limitations on obtained results could not be ruled out.

  16. Verification and quality control of routine hematology analyzers.

    Science.gov (United States)

    Vis, J Y; Huisman, A

    2016-05-01

    Verification of hematology analyzers (automated blood cell counters) is mandatory before new hematology analyzers may be used in routine clinical care. The verification process consists of several items which comprise among others: precision, accuracy, comparability, carryover, background and linearity throughout the expected range of results. Yet, which standard should be met or which verification limit be used is at the discretion of the laboratory specialist. This paper offers practical guidance on verification and quality control of automated hematology analyzers and provides an expert opinion on the performance standard that should be met by the contemporary generation of hematology analyzers. Therefore (i) the state-of-the-art performance of hematology analyzers for complete blood count parameters is summarized, (ii) considerations, challenges, and pitfalls concerning the development of a verification plan are discussed, (iii) guidance is given regarding the establishment of reference intervals, and (iv) different methods on quality control of hematology analyzers are reviewed. © 2016 John Wiley & Sons Ltd.

  17. The European Hematology Association Roadmap for European Hematology Research: a consensus document.

    Science.gov (United States)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke; Coiffier, Bertrand; Cordonnier, Catherine; Döhner, Hartmut; de Wit, Thom Duyvené; Eichinger, Sabine; Fibbe, Willem; Green, Tony; de Haas, Fleur; Iolascon, Achille; Jaffredo, Thierry; Rodeghiero, Francesco; Salles, Gilles; Schuringa, Jan Jacob

    2016-02-01

    The European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European hematology research. Initiated by the EHA, around 300 experts contributed to the consensus document, which will help European policy makers, research funders, research organizations, researchers, and patient groups make better informed decisions on hematology research. It also aims to raise public awareness of the burden of blood disorders on European society, which purely in economic terms is estimated at €23 billion per year, a level of cost that is not matched in current European hematology research funding. In recent decades, hematology research has improved our fundamental understanding of the biology of blood disorders, and has improved diagnostics and treatments, sometimes in revolutionary ways. This progress highlights the potential of focused basic research programs such as this EHA Roadmap.The EHA Roadmap identifies nine 'sections' in hematology: normal hematopoiesis, malignant lymphoid and myeloid diseases, anemias and related diseases, platelet disorders, blood coagulation and hemostatic disorders, transfusion medicine, infections in hematology, and hematopoietic stem cell transplantation. These sections span 60 smaller groups of diseases or disorders.The EHA Roadmap identifies priorities and needs across the field of hematology, including those to develop targeted therapies based on genomic profiling and chemical biology, to eradicate minimal residual malignant disease, and to develop cellular immunotherapies, combination treatments, gene therapies, hematopoietic stem cell treatments, and treatments that are better tolerated by elderly patients. Copyright© Ferrata Storti Foundation.

  18. A comparative study of the hematological values in the ovulation ...

    African Journals Online (AJOL)

    This study compares the hematological values of the ovulation phase with the luteal phase of the menstrual cycle. Sixty subjects with 30 subjects each in the ovulation and luteal phases of the menstrual cycle were studied. Ethical clearance and informed consent were obtained for the study. Hematological parameters of ...

  19. HEMATOLOGICAL INDICES OF RAT ORGANISMS UNDER CONDITIONS OF OXIDATIVE STRESS AND LIPOSOMAL PREPARATION ACTION

    Directory of Open Access Journals (Sweden)

    M. Khariv

    2016-04-01

    Full Text Available The article deals with the results of search of the influence of developed complex liposomal drug on dynamics of hematological parameters of rat organisms under conditions of simulated oxidative stress caused by the use of carbon tetrachloride. Intramuscular injection of 50% tetrachloromethane to rats at a dose of 0.25 ml per 100 g of body weight causes antigenic load on the body and leads to disruption of physiologic level of hematological indices of experimental animal organisms. This indicates the number reduce of red blood cells, hemoglobin content, hemoglobin concentration in erythrocyte, increasing the number of leukocytes, mass of hemoglobin in erythrocyte and increased of color index. To normalize the hematological indices of rat organisms for the development of oxidative stress it is advisable to apply the liposomal drug that incorporates contains butafosfan, interferon, milk thistle and vitamins. When using liposomal drug to rats, under conditions of oxidative stress, the normalization of hematological indices comes in blood, namely on 14th day within physiological variables were indicators of the number of erythrocytes of hemoglobin content, white blood cell count and indices of red blood cells compared to controls, indicating a recovery of hematopoietic function of marrow.

  20. A Novel Multivariate Generalized Skew-Normal Distribution with Two Parameters BGSNn, m (λ1, λ2

    Directory of Open Access Journals (Sweden)

    Fathi B.

    2014-07-01

    Full Text Available In this paper we first introduce a new class of multivariate generalized asymmetric skew-normal distributions with two parameters λ1,λ2 that we present it by BGSNn, m (λ1,λ2, and we finally obtain some special properties of BGSNnm(λ1,λ2.

  1. A Novel Multivariate Generalized Skew-Normal Distribution with Two Parameters BGSNn, m (λ1, λ2)

    OpenAIRE

    Fathi B.; Hasanalipour P.

    2014-01-01

    In this paper we first introduce a new class of multivariate generalized asymmetric skew-normal distributions with two parameters λ1,λ2 that we present it by BGSNn, m (λ1,λ2), and we finally obtain some special properties of BGSNnm(λ1,λ2).

  2. Effects of normal and extreme turbulence spectral parameters on wind turbine loads

    DEFF Research Database (Denmark)

    Dimitrov, Nikolay Krasimirov; Natarajan, Anand; Mann, Jakob

    2017-01-01

    Loads simulations as performed to obtain design loads on wind turbines, requires wind turbulence as an input, characterized by parameters associated with the turbulence length scale, dissipation and anisotropy. The effect of variation in these turbulence spectral parameters on the magnitude...... the recommended values in the IEC 61400-1 Ed.3 that is used for wind turbine design. The present paper investigates the impact of Mann turbulence model parameter variations on the design loads envelope for 5 MW and 10 MW reference wind turbines. Specific focus is made on the blade root loads, tower top moments...

  3. The effects of combination of Eurycoma longifolia Jack ethanolic extract and doxorubicine on hematological profile in rats given by 7,12-dimethylbenz(a)anthracene

    Science.gov (United States)

    Nurani, L. H.; Mursyidi, A.; Widyarini, S.; Rohman, A.

    2017-11-01

    Doxorubicin (Dox) is known as anticancer drug commonly used for cancer treatment. Eurycoma longifolia Jack or Pasakbumi was reported to have chemopreventive effect. In cancer patients, there are some dysfunctions of blood parameter, therefore some hematologic tests are needed to monitor cancer patients. In this study, the effects of combination of ethanolic extract of E. longifolia Jack (EEE) and Dox on hematologic profiles were investigated in rats injected by DMBA. Rats were divided into eight groups. Group I was normal group; Group II, rats were treated with extract dose 100 mg/kgbw; Groups III, IV, V, VI, VII and VIII, rats were treated with Dox, DMBA, DMBA+Dox, DMBA+EEE, DMBA+Dox +EEE, and Dox+EEE, respectively. DMBA administration orally was conducted twice a week for 5 weeks. At 16th week of treatments, bloods were taken from orbitalis sinus for hematologicals profile (levels of Hb, erytrocyte, hematocrite, leukocyte, MCV, MCH, and differencial leucocyte count) measurements. These data were analyzed by one way ANOVA followed by LSD test. DMBA administration significantly decreased the hematological profiles compared to the normal group, except in lymphocyte level. Rats treated with extract and extract+Dox were able to increase the hematological profile compared to rats given by DMBA only. Based on these findings it can be concluded that the combination of EEE and Dox potentially increase hematological profile of rats given by DMBA.

  4. Connection between Dynamically Derived Initial Mass Function Normalization and Stellar Population Parameters

    NARCIS (Netherlands)

    McDermid, Richard M.; Cappellari, Michele; Alatalo, Katherine; Bayet, Estelle; Blitz, Leo; Bois, Maxime; Bournaud, Frédéric; Bureau, Martin; Crocker, Alison F.; Davies, Roger L.; Davis, Timothy A.; de Zeeuw, P. T.; Duc, Pierre-Alain; Emsellem, Eric; Khochfar, Sadegh; Krajnović, Davor; Kuntschner, Harald; Morganti, Raffaella; Naab, Thorsten; Oosterloo, Tom; Sarzi, Marc; Scott, Nicholas; Serra, Paolo; Weijmans, Anne-Marie; Young, Lisa M.

    We report on empirical trends between the dynamically determined stellar initial mass function (IMF) and stellar population properties for a complete, volume-limited sample of 260 early-type galaxies from the ATLAS3D project. We study trends between our dynamically derived IMF normalization αdyn ≡

  5. Parameter Estimation under Constraints for Multivariate Normal Distributions with Incomplete Data.

    Science.gov (United States)

    Zoppe, Alice; Buu, Yuh-Pey Anne; Flury, Bernard

    2001-01-01

    Presents an application of the EM-algorithm to two problems of estimation and testing in a multivariate normal distribution with missing data. The two models are tested applying the log-likelihood ratio test. Solves the problem of different and nonmonotone patterns of missing data by introducing suitable transformations and partitions of the data…

  6. Performance, immunity, serum biochemical and hematological ...

    African Journals Online (AJOL)

    ... results suggest that supplementing broilers' diet with 5 g/kg thyme can indicate favorable influences of antibiotic growth promoter on performance without any detrimental impacts on immune responses and blood parameters. Key words: Broiler, thyme, growth performance, immunity, serum biochemistry, hematology.

  7. Effects of exercise and milk fat globule membrane (MFGM supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.

    Directory of Open Access Journals (Sweden)

    Hunkyung Kim

    Full Text Available To investigate the combined and separate effects of exercise and milk fat globule membrane (MFGM supplementation on frailty, physical function, physical activity level, and hematological parameters in community-dwelling elderly Japanese women.A total of 131 frail, elderly women over 75 years were randomly assigned to one of four groups: exercise and MFGM supplementation (Ex+MFGM, exercise and placebo (Ex+Plac, MFGM supplementation, or the placebo group. The exercise group attended a 60-minute training program twice a week for three months, and the MFGM group ingested 1g of the MFGM supplement in pill form, daily for 3 months. The primary outcome measure was change in frailty status based on Fried's frailty phenotype. Secondary outcome measures included body composition, physical function and hematological parameters, and interview survey components assessing lifestyle factors. Participants were followed for 4 months post-intervention.Significant group × time interactions were observed for usual walking speed (P = 0.005, timed up & go (P<0.001, and insulin-like growth factor-binding protein 3/insulin-like growth factor 1 ratio (P = 0.013. The frailty components revealed that weight loss, exhaustion, low physical activity, and slow walking speed were reversed, but low muscle strength did not significantly changed. Frailty reversal rate was significantly higher in the Ex+MFGM (57.6% than in the MFGM (28.1% or placebo (30.3% groups at post-intervention (χ2 = 8.827, P = 0.032, and at the follow-up was also significantly greater in the Ex+MFGM (45.5% and Ex+Plac (39.4% groups compared with the placebo (15.2% group (χ2 = 8.607, P = 0.035. The exercise+MFGM group had the highest odds ratio (OR for frailty reversal at post-intervention and follow-up (OR = 3.12, 95% confidence interval (CI = 1.13-8.60; and OR = 4.67, 95% CI = 1.45-15.08, respectively.This study suggests that interventions including exercise and nutrition can improve frailty status

  8. Hematological profile of sickle cell disease from South Gujarat, India

    Directory of Open Access Journals (Sweden)

    Sanjeev Shyam Rao

    2012-05-01

    Full Text Available The aim of this study was to determine hematological profile of sickle cell disease (SCD from Surat, South Gujarat, India. This prospective cross-sectional study was conducted in the Department of Pediatrics and Sickle Cell Anemia Laboratory, Faculty of Pathology, Government Medical College, Surat, India, between July 2009 and December 2010. Patients included in this study were in their steady state for a long period of time without any symptoms related to SCD or other diseases which could affect the hematological parameters. Venous blood of all patients was collected in ethylenediaminetetraacetic acid and hematological indices were measured. Thirty-three subjects homozygous in all were studied for their hematological parameters for sickle cell anemia. Moderate to severe anemia, low mean cell volume and high foetal hemoglobin dominate the hematological profile of SCD children.

  9. Hematologic and plasma biochemical reference values in Indian peafowl (Pavo cristatus).

    Science.gov (United States)

    Samour, Jaime; Naldo, Jesus; Rahman, Habeeb; Sakkir, Mohammed

    2010-06-01

    Blood samples were collected from captive, adult, clinically normal Indian peafowl (Pavo cristatus) for hematologic and plasma biochemical analyses. Hematologic parameters investigated were total red blood cell count, hemoglobin, packed cell volume, fibrinogen, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, total white blood cell count, differential white blood cell count, and thrombocyte count. Plasma biochemical parameters investigated were alanine aminotransferase, alkaline phosphatase, amylase, aspartate aminotransferase, bile acids, total bilirubin, blood urea nitrogen, calcium, cholesterol, creatinine, creatine kinase, gamma glutamyltransferase, lactate dehydrogenase, glucose, iron, phosphorus, and uric acid, as well as plasma protein electrophoresis. Results were compared with values from studies done in houbara bustards (Chlamydotis undulata), kori bustards (Ardeotis kori), stone curlews (Burhinus oedicnemus), and taxonomically related species, including ring-necked pheasants (Phasianus colchicus), red-legged partridges (Alectoris rufa), Kashmir native fowl (Kashmirfavorella), and Bangladesh native, Fayoumi, and Assil fowl (Gallus domesticus).

  10. Gonadosomatic index and some hematological parameters in African catfish Clarias gariepinus (Burchell, 1822 as affected by feed type and temperature level

    Directory of Open Access Journals (Sweden)

    Waleed A. Al-Deghayem

    Full Text Available ABSTRACT The purpose of the current study was to examine the effects of different feeds/nutrients and temperature on the gonadal development of Clarias gariepinus. The gonadosomatic index (GSI and blood parameters including red blood cell count (RBCs, white blood cell count (WBCs, hemoglobin (Hgb level, hematocrit (HCT, platelets (PLT and mean corpuscular volume (MCV were investigated. Four types of fish feed of 36% protein content [D1(fish meal, D2 (soybean meal, D3 (peameal and D4 (commercial tilapia feed] and 3 different levels of temperature (T1(24℃, T2(28℃ and T3(32℃ were tried in this study for a duration of 4 months. The mean values were as follows: female GSI (14.68 ± 4.86, male GSI (0.70 ± 0.32, RBCs x 106(2.45± 0.64, WBCs x 103(9.28 ± 2.34, Hgb (12.42± 2.21g/dl, HCT (29.06 ± 3.54%, PLT (90.75 ± 9.18/mm3, and MCV (118.08 ± 10.3 g/l. Fish meal diet revealed the most significant (p<0.05 increase in weight gain, female GSI, and also exerted significant increases on most of the blood parameters. This study revealed that animal-based protein diet and temperature around 28℃ were the critical requirements for the physiological performance and relative gonadal weight of C. gariepinus. GSI and blood parameters were useful indicators of stress exerted by nutrition and temperature on fish, and their study is critical for fish health and mass production of viable seeds for aquaculture enterprise.

  11. Evaluation of moving-coil loudspeaker and passive radiator parameters using normal-incidence sound transmission measurements: theoretical developments.

    Science.gov (United States)

    Leishman, Timothy W; Anderson, Brian E

    2013-07-01

    The parameters of moving-coil loudspeaker drivers are typically determined using direct electrical excitation and measurement. However, as electro-mechano-acoustical devices, their parameters should also follow from suitable mechanical or acoustical evaluations. This paper presents the theory of an acoustical method of excitation and measurement using normal-incidence sound transmission through a baffled driver as a plane-wave tube partition. Analogous circuits enable key parameters to be extracted from measurement results in terms of open and closed-circuit driver conditions. Associated tools are presented that facilitate adjacent field decompositions and derivations of sound transmission coefficients (in terms of driver parameters) directly from the circuits. The paper also clarifies the impact of nonanechoic receiving tube terminations and the specific benefits of downstream field decompositions.

  12. Parameter Recovery for the 1-P HGLLM with Non-Normally Distributed Level-3 Residuals

    Science.gov (United States)

    Kara, Yusuf; Kamata, Akihito

    2017-01-01

    A multilevel Rasch model using a hierarchical generalized linear model is one approach to multilevel item response theory (IRT) modeling and is referred to as a one-parameter hierarchical generalized linear logistic model (1-P HGLLM). Although it has the flexibility to model nested structure of data with covariates, the model assumes the normality…

  13. Dehydroepiandrosterone substitution in female adrenal failure: no impact on endothelial function and cardiovascular parameters despite normalization of androgen status

    DEFF Research Database (Denmark)

    Christiansen, Jens Juel; Andersen, Niels Holmark; Sørensen, Keld E

    2007-01-01

    because of skin side effects and anxiety, respectively. All patients had low circulating androgens baseline and normal range androgens during DHEA treatment. We examined patients with noninvasive endothelial cell function, magnetic resonance imaging (MRI)-based cardiac output, echocardiography, ambulatory...... 24-h blood pressure and maximal oxygen consumption. RESULTS: DHEA treatment normalized androgen status to levels seen in healthy women. DHEA and placebo treatment had no effect on echocardiographic parameters of myocardial dimensions or systolic and diastolic function, noninvasive endothelial cell...... in vascular endothelium has been described and in vitro studies have shown involvement of DHEA in NO dependent pathways. AIM: To evaluate effects of DHEA substitution on cardiovascular parameters. DESIGN: Six months randomized, double-blind, placebo-controlled crossover study. Treatment consisted of DHEA 50...

  14. Study of Hematological Parameters in Children Suffering from Iron Deficiency Anaemia in Chattagram Maa-o-Shishu General Hospital, Chittagong, Bangladesh

    Science.gov (United States)

    Mohammed Mujib, Abu Syed; Halder, Milton; Monirul Hasan, Chowdhury Mohammad

    2014-01-01

    A total of 150 (30.61%) anemic patients out of 490 patients diagnosed to have iron deficiency anemia (IDA) have been selected for the first time in Bangladesh. For detailed study, blood samples from 150 anemic patients along with 25 controls were analyzed. Analysis of variance showed significant P value between mean platelet volume (MPV) in females (8.08 μm3) and males (7.59 μm3) (P iron deficiency anemia patients. Besides, the value of white blood cells (WBC) in males (10946.08/cmm) was significantly higher than in females (9470.833/cmm) (P anemias with only one of three biochemical parameters decreased and control showed that this group does not have iron deficiency. PMID:25400943

  15. Effect of Various Concentrations of an Anise Seed Powder (Pimpinella Anisum L. Supplement on Selected Hematological and Biochemical Parameters of Broiler Chickens

    Directory of Open Access Journals (Sweden)

    KIA Al-Shammari

    Full Text Available ABSTRACT The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L. powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control, T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L. The following serum biochemical parameters were evaluated: CHOL, GLU, TP, albumin, globulins, Ca, P, TG, total lipids, UA, and creatinine, as well as AST and ALT enzyme activities. The statistical analysis indicated that the anise supplement significantly improved blood RBC, WBC, Hgb, HCT, TP, albumin, globulin, GLU, P, and Ca levels of broilers in groups T4, T3, and T2 compared with the control group on days 28 and 56, and on average. Also, T3, T2 and T1 presented lower H/L ratio and CHOL, TG, total lipids, creatinine, UA, AST and ALT serum levels compared with the control group. The highest inclusion levels of anise seed powder, i.e., 1000 and 750mg/L, had a stimulating effect on the physiological traits of the birds.

  16. Study of the characteristic parameters of the normal voices of Argentinian speakers

    Directory of Open Access Journals (Sweden)

    Edgardo Bonzi

    2014-02-01

    Full Text Available The voice laboratory permits to study the human voices using a method that is objective and noninvasive. In this work, we have studied the parameters of the human voice such as pitch, formant, jitter, shimmer and harmonic-noise ratio of a group of young people. This statistical information of parameters is obtained from Argentinian speakers. Received: 29 December 2013, Accepted: 27 May 2014; Reviewed by: J. Brum, Instituto de Física, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; Edited by: E. Mizraji; DOI: http://dx.doi.org/10.4279/PIP.060002 Cite as: E V Bonzi, G B Grad, A M Maggi, M R Muñoz, Papers in Physics 6, 060002 (2014

  17. Cathode fall parameters of a self-sustained normal glow discharge in atmospheric-pressure helium

    International Nuclear Information System (INIS)

    Arkhipenko, V.I.; Zgirovskii, S.M.; Kirillov, A.A.; Simonchik, L.V.

    2002-01-01

    Results from comprehensive studies of a high-current self-sustained glow discharge in atmospheric-pressure helium are presented. The main parameters of the cathode fall, namely, the electric field profile, cathode fall thickness, current density, gas temperature, and heat flux to the cathode are determined. The results obtained are discussed using one-dimensional models of the cathode fall with allowance for volumetric heat release

  18. Ocular biometric parameters associated with intraocular pressure reduction after cataract surgery in normal eyes.

    Science.gov (United States)

    Yang, Hyun Seung; Lee, Junsung; Choi, Sangkyung

    2013-07-01

    To evaluate the ocular biometric parameters associated with intraocular pressure (IOP) reduction after phacoemulsification. Prospective, observational case series. The study included 999 patients who had undergone uncomplicated phacoemulsification. IOP and ocular biometric parameters were checked preoperatively and 3 months postoperatively using anterior segment optical coherence tomography, optical biometry, and ultrasonic biomicroscopy. The relationship between IOP change and the parameters, including preoperative IOP, anterior chamber depth, axial length, angle opening distance at 500 μm, anterior chamber area, corneal thickness, lens thickness, and iris thickness at 750 μm, was evaluated. The mean patient age was 67.1 ± 4.3 years. The average change in IOP was -1.6 mm Hg (-11.8%). In univariate analysis, axial length, corneal thickness, and iris thickness were not significantly associated with IOP reduction. However, preoperative IOP, anterior chamber depth, angle opening distance, anterior chamber area, and lens thickness were significantly associated with IOP change (P < .05). Furthermore, changes in anterior chamber depth (standardized coefficient beta [B] = -0.082), angle opening distance (B = -0.095), and anterior chamber area (B = -0.380) were more strongly correlated with IOP change than were preoperative factors (B = -0.078, B = -0.071, and B = -0.067, respectively). In multivariate analysis, preoperative IOP, lens thickness, angle opening distance change, and anterior chamber area change were significantly associated with IOP change (P < .005). In addition to preoperative IOP and lens thickness, parameters such as changes in anterior chamber area and angle opening distance were significantly associated positively with reduced IOP after phacoemulsification. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. [Hemostatic system parameters of placental extracts in normal pregnancy and severe preeclampsia].

    Science.gov (United States)

    López-Ramírez, Ysabel; Carvajal, Zoila; Arocha-Piñango, Carmen Luisa

    2006-09-01

    To better understand the role of the hemostatic mechanism in preeclampsia, placental extracts obtained from 26 normal pregnant women (NP) and 12 patients with severe pre-eclampsia (SPE) were analyzed to determine thrombomodulin (TM), tissue factor (TF), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor (PAI) 1 and 2, and TF pathway inhibitor (TFPI). The results showed similar concentrations of TF, TM and PAI-2 in both groups, while tPA increased no significantly and TFPI and PAI-1 increased significantly in SPE placentas.

  20. Normal values and standardization of parameters in nuclear cardiology: Japanese Society of Nuclear Medicine working group database.

    Science.gov (United States)

    Nakajima, Kenichi; Matsumoto, Naoya; Kasai, Tokuo; Matsuo, Shinro; Kiso, Keisuke; Okuda, Koichi

    2016-04-01

    As a 2-year project of the Japanese Society of Nuclear Medicine working group activity, normal myocardial imaging databases were accumulated and summarized. Stress-rest with gated and non-gated image sets were accumulated for myocardial perfusion imaging and could be used for perfusion defect scoring and normal left ventricular (LV) function analysis. For single-photon emission computed tomography (SPECT) with multi-focal collimator design, databases of supine and prone positions and computed tomography (CT)-based attenuation correction were created. The CT-based correction provided similar perfusion patterns between genders. In phase analysis of gated myocardial perfusion SPECT, a new approach for analyzing dyssynchrony, normal ranges of parameters for phase bandwidth, standard deviation and entropy were determined in four software programs. Although the results were not interchangeable, dependency on gender, ejection fraction and volumes were common characteristics of these parameters. Standardization of (123)I-MIBG sympathetic imaging was performed regarding heart-to-mediastinum ratio (HMR) using a calibration phantom method. The HMRs from any collimator types could be converted to the value with medium-energy comparable collimators. Appropriate quantification based on common normal databases and standard technology could play a pivotal role for clinical practice and researches.

  1. Symmetry of retinal parameters measured by spectral-domain OCT in normal young adults.

    Science.gov (United States)

    Dalgliesh, James D; Tariq, Yasser M; Burlutsky, George; Mitchell, Paul

    2015-01-01

    To determine interocular differences in retinal parameters in a population of young adults using spectral-domain optical coherence tomography. In the Sydney Adolescent Vascular Eye Study, young adults aged 16 to 19 years received a standardized eye examination encompassing retinal photography, spectral-domain optical coherence tomography (Cirrus HD-OCT; Carl Zeiss Meditec, Dublin, CA) and best-corrected visual acuity. Interocular differences in retinal nerve fiber layer (RNFL) and macular parameters together with sex and ethnic variations for these differences were determined. In the 1500 subjects tested, right eyes had thicker average RNFL than left eyes (0.3 μm, P=0.0074). There were no significant interocular differences in central macular or macular cube average thickness. After adjusting for interocular difference in axial length, right eyes had slightly smaller total macular volume than left eyes (-0.0082 mm, P=0.008). The 2.5% to 97.5% limits of asymmetry were 9.3 μm for average RNFL, 8 μm for average macular thickness, and 0.20 mm for total macular volume. In the outer inferior quadrant, there was a greater macular thickness asymmetry in females than males (-0.7 μm, P=0.037). In the inferior RNFL quadrant (-2.6 μm, P=0.0013) and in the outer inferior macular quadrant (1.6 μm, P=0.0018), white individuals had greater symmetry than East Asians. In this study, we report the degree of interocular symmetry of RNFL and macular parameters measured by Cirrus HD-OCT in healthy young adults.

  2. Sperm vacuoles cannot help to differentiate fertile men from infertile men with normal sperm parameter values.

    Science.gov (United States)

    Gatimel, N; Léandri, R D; Marino, L; Esquerre-Lamare, C; Parinaud, J

    2014-11-01

    Can the assessment of sperm vacuoles at high magnification contribute to the explanation of idiopathic infertility? The characteristics of sperm head vacuoles (number, area, position) are no different between fertile controls and patients with unexplained infertility. Until now, the assessment of sperm head vacuoles has been focused on a therapeutic goal in the intracytoplasmic morphologically selected sperm injection (IMSI) procedure, but it could be pertinent as a new diagnostic tool for the evaluation of male fertility. This diagnostic test study with blind assessment included a population of 50 fertile men and 51 men with idiopathic infertility. They were selected from September 2011 to May 2013. Fertile men were within couples who had a spontaneous pregnancy in the last 2 years. Infertile men were within couples who had unexplained infertility and were consulting in our centre. After analysis of conventional sperm parameters, we investigated the number, position and area of sperm head vacuoles at high magnification (×6000) with interference contrast using an image analysis software. We also carried out a nuclear status analysis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling assay (TUNEL), sperm chromatin structure assay (SCSA) and aniline blue staining. Concerning the vacuoles data, we did not find any significant difference between the two populations. We found no significant correlation between the vacuolar parameters (mean number of vacuoles, relative vacuole area and percentage of spermatozoa with large vacuoles) and either conventional semen parameters, male age or the data from the aniline blue staining, SCSA assay and TUNEL assay. Despite the fact all of the vacuole parameters values were identical in fertile and infertile men, we cannot totally exclude that a very small cause of unexplained infertilities could be related to an excess of sperm vacuoles. In line with its widely debated use as a therapeutic tool, sperm vacuole

  3. The corneoscleral shell of the eye: potentials of assessing biomechanical parameters in normal and pathological conditions

    Directory of Open Access Journals (Sweden)

    E. N. Iomdina

    2016-01-01

    Full Text Available The paper reviews modern methods of evaluating the biomechanical properties of the corneoscleral shell of the eye that can be used both in the studies of the pathogenesis of various ophthalmic pathologies and in clinical practice. The biomechanical parameters of the cornea and the sclera have been shown to be diagnostically significant in assessing the risk of complications and the effectiveness of keratorefractive interventions, in the diagnosis and the prognosis of keratoconus, progressive myopia, or glaucoma. In clinical practice, a special device, Ocular Response Analyzer (ORA, has been used on a large scale. The analyzer is used to assess two parameters that characterize viscoelastic properties of the cornea — corneal hysteresis (CH and corneal resistance factor (CRF. Reduced levels of CH and CRF have been noted after eximer laser surgery, especially that administered to patients who demonstrate a regression in the refraction effect or suffer from keratoconus. This fact justifies the use of these biomechanical parameters as additional diagnostic criteria in the evaluation of the state of the cornea. At the same time, ORA data are shown to reflect the biomechanical response to the impact of the air pulse not only from the cornea alone but also from the whole corneoscleral capsule. This is probably the cause of reduced CH in children with progressive myopia and a weakened supportive function of the sclera, as well as such reduction in glaucomatous adult patients. It is hypothesized that a low CH value is a result of remodeling of the connective tissue matrix of the corneoscleral shell of the eye and can be an independent factor testifying to a risk of glaucoma progression. Reduced CH in primary open-angle glaucoma occurs in parallel with the development of pathological structural changes of the optic disc, and deterioration of visual fields, which is an evidence of a specific character and sensitivity of this parameter. The

  4. Assessing gait variability in transtibial amputee fallers based on spatial-temporal gait parameters normalized for walking speed.

    Science.gov (United States)

    Hordacre, Brenton G; Barr, Christopher; Patritti, Benjamin L; Crotty, Maria

    2015-06-01

    To determine whether normalizing spatial-temporal gait data for walking speed obtained from multiple walking trials leads to differences in gait variability parameters associated with a history of falling in people with transtibial amputations. Cross-sectional study. Rehabilitation center. People with unilateral transtibial amputations (N=45; mean age ± SD, 60.5±13.7y; 35 men [78%]) were recruited. Not applicable. Participants completed 10 consecutive walking trials using an instrumented walkway system. Primary gait parameters were walking speed and step-length, step-width, step-time, and swing-time variability. A retrospective 12-month fall history was obtained from participants. Sixteen amputees (36%) were classified as fallers. Variation in gait speed across the 10 walking trials was 2.9% (range, 1.1%-12.1%). Variability parameters of normalized gait data were significantly different from variability parameters of nonnormalized data (all Prehabilitation efforts of amputees with a fall history. Copyright © 2015 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.

  5. α-Lipoic Acid Mitigates Arsenic-Induced Hematological Abnormalities in Adult Male Rats

    Directory of Open Access Journals (Sweden)

    Sonali Ghosh

    2017-05-01

    Full Text Available Background: Arsenic toxicity is a major global health problem and exposure via contaminated drinking water has been associated with hematological and other systemic disorders. The present investigation has been conducted in adult male rats to evaluate the protective ability of α-lipoic acid (ALA against such hematological disorders. Methods: Twenty-four adult male Wister rats (b.wt.130±10g were grouped and accordingly group I (control received the normal diet, group II (treated was given arsenic orally for 28 consecutive days as arsenic trioxide (3 mg/kgbw/rat/day whereas group III (supplemented received the same dose of arsenic along with ALA (25 mg/kgbw/rat/day as oral supplement. Hematological profile, plasma oxidant/antioxidant status, and erythrocyte morphology were assessed. Statistical analysis was done by one-way ANOVA using SPSS software (version 16.0. Results: Arsenic exposure caused reduction of erythrocyte (P=0.021, leucocyte (P<0.001, and hemoglobin (P=0.031 associated with echinocytic transformation as evidenced by light and scanning electron microscopic studies. The other significantly altered parameters include increased mean corpuscular volume (P=0.041 and lymphocytopenia (P<0.001 with insignificant neutropenia and eosinophilia. Altered serum oxidative balance as evidenced by decreased TAS (P<0.001 and increased TOS (P<0.001 with OSI (P<0.001 was also noted. The dietary supplementation of ALA has a beneficial effect against the observed (P<0.05 arsenic toxicities. It brings about the protection by restoring the hematological redox and inflammatory status near normal in treated rats. Arsenic-induced morphological alteration of erythrocytes was also partially attenuated by ALA supplementation. Conclusion: It is concluded that arsenicosis is associated with hematological alterations and ALA co-supplementation can partially alleviate these changes in an experimental male rat model.

  6. Normal Force Influence on 3D Texture Parameters Characterizing the Friction Couple Steel – PBT + 10 % PTFE

    Directory of Open Access Journals (Sweden)

    C. Georgescu

    2014-03-01

    Full Text Available This study presents the influence of the normal force on the surface quality of the friction couple steel – polybutylene terephthalate (PBT + 10 % polytetrafluoroethylene (PTFE. There were calculated the average values of the amplitude and functional parameters, as obtained from investigating square areas on the wear tracks, with the help of a proposed methodology, for initial and tested surfaces generated on the blocks and on counterpart ring made of rolling bearing steel, for the following test conditions: three normal forces (F = 1 N, F = 2.5 N and F =5 N, three sliding speeds (v = 0.25 m/s, v = 0.50 m/s and v = 0.75 m/s and a sliding distance of L = 7500 m. The conclusion of the research study was that the tested normal force range has an insignificant influence on the surface quality for the tested materials and parameters. This friction couple could be recommended for variable conditions (speed and load in dry regimes.

  7. Tracheal sound parameters of respiratory cycle phases show differences between flow-limited and normal breathing during sleep

    International Nuclear Information System (INIS)

    Kulkas, A; Huupponen, E; Virkkala, J; Saastamoinen, A; Rauhala, E; Tenhunen, M; Himanen, S-L

    2010-01-01

    The objective of the present work was to develop new computational parameters to examine the characteristics of respiratory cycle phases from the tracheal breathing sound signal during sleep. Tracheal sound data from 14 patients (10 males and 4 females) were examined. From each patient, a 10 min long section of normal and a 10 min section of flow-limited breathing during sleep were analysed. The computationally determined proportional durations of the respiratory phases were first investigated. Moreover, the phase durations and breathing sound amplitude levels were used to calculate the area under the breathing sound envelope signal during inspiration and expiration phases. An inspiratory sound index was then developed to provide the percentage of this type of area during the inspiratory phase with respect to the combined area of inspiratory and expiratory phases. The proportional duration of the inspiratory phase showed statistically significantly higher values during flow-limited breathing than during normal breathing and inspiratory pause displayed an opposite difference. The inspiratory sound index showed statistically significantly higher values during flow-limited breathing than during normal breathing. The presented novel computational parameters could contribute to the examination of sleep-disordered breathing or as a screening tool

  8. The role of cytokines in development of hematological and immune disorders at radiation therapy for uterine body cancer

    International Nuclear Information System (INIS)

    Sorochan, P.P.; Prokhach, N.E.; Gromakova, Yi.A.; Krugova, Yi.M.; Sukhyin, V.S.

    2013-01-01

    The changes in hematological and immune parameters in patients with uterine body cancer were analyzed by the stages of the combined treatment. The rol of cytokines in the development of hematologic and immune disorders was assessed

  9. Normalization in Unsupervised Segmentation Parameter Optimization: A Solution Based on Local Regression Trend Analysis

    Directory of Open Access Journals (Sweden)

    Stefanos Georganos

    2018-02-01

    Full Text Available In object-based image analysis (OBIA, the appropriate parametrization of segmentation algorithms is crucial for obtaining satisfactory image classification results. One of the ways this can be done is by unsupervised segmentation parameter optimization (USPO. A popular USPO method does this through the optimization of a “global score” (GS, which minimizes intrasegment heterogeneity and maximizes intersegment heterogeneity. However, the calculated GS values are sensitive to the minimum and maximum ranges of the candidate segmentations. Previous research proposed the use of fixed minimum/maximum threshold values for the intrasegment/intersegment heterogeneity measures to deal with the sensitivity of user-defined ranges, but the performance of this approach has not been investigated in detail. In the context of a remote sensing very-high-resolution urban application, we show the limitations of the fixed threshold approach, both in a theoretical and applied manner, and instead propose a novel solution to identify the range of candidate segmentations using local regression trend analysis. We found that the proposed approach showed significant improvements over the use of fixed minimum/maximum values, is less subjective than user-defined threshold values and, thus, can be of merit for a fully automated procedure and big data applications.

  10. The Reproduction Performance of Tegal Duck Based on Hematology Status

    Directory of Open Access Journals (Sweden)

    Ismoyowati Ismoyowati

    2006-05-01

    Full Text Available The experiment was conducted to study the physiological indicator of Tegal duck based on the hematological parameters and its relation to reproduction performances.  The research materials were 20 heads of male and 100 heads of female Tegal ducks. Parameters measured were erytrocyte, leucocyte, differential leucocyte, hemoglobin, hematocrit, protein total, albumin and globulin, and reproduction performances (semen volume and egg production.  Method of the research was experimental with Completely Randomized Design,  and data were analysed using analysis of variance and correlation.  The result of the experiment showed that hematological parameters of Tegal duck that had high production were high significantly (P<0,05 different than the others, except on albumin value.  It was concluded that reprodution performances of Tegal duck was highly affected by its hematologys status. (Animal Production 8(2: 88-93 (2006   Key Words: Reproduction performances, Tegal duck, hematology status

  11. Normal ocular parameters and characterization of ophthalmic lesions in a group of captive bald eagles (Haliaeetus leucocephalus).

    Science.gov (United States)

    Kuhn, Sonia E; Jones, Michael P; Hendrix, Diane V H; Ward, Daniel A; Baine, Katherine H

    2013-06-01

    Sixteen adult captive bald eagles (Haliaeetus leucocephalus) underwent a complete bilateral ocular examination to assess normal ocular parameters and describe ophthalmic lesions. Tear production was measured with the Schirmer tear test 1 and intraocular pressure was measured with applanation tonometry. The menace response was normal bilaterally in 13 of 16 eagles. Two birds had normal menace responses despite having fundic lesions, and 2 birds with an inconsistent or absent menace response did not have appreciable ophthalmic lesions. Mean (SD) tear production was 14 +/- 2 mm/min (range, 8-19 mm/min). Mean intraocular pressure was 21.5 +/- 1.7 mm Hg (range, 15-26 mm Hg). At least 1 ocular lesion was present in 50% of examined eyes. Cataracts, the most common lesion observed, were present in 8 eyes of 5 birds. Three of 4 known geriatric birds were or had been affected with bilateral cataracts. Overall, ocular lesions are common in captive bald eagles, and cataracts appear to be more prevalent in geriatric bald eagles. An obvious positive menace response is present in most visual birds but may be absent in some eagles that are either normal or that do not have appreciable ophthalmic lesions. Applanation tonometry and the Schirmer tear test 1 can be performed easily on adult bald eagles and provide reproducible results.

  12. Blood coagulation parameters and platelet indices: changes in normal and preeclamptic pregnancies and predictive values for preeclampsia.

    Directory of Open Access Journals (Sweden)

    Lei Han

    Full Text Available Preeclampsia (PE is an obstetric disorder with high morbidity and mortality rates but without clear pathogeny. The dysfunction of the blood coagulation-fibrinolysis system is a salient characteristic of PE that varies in severity, and necessitates different treatments. Therefore, it is necessary to find suitable predictors for the onset and severity of PE.We aimed to evaluate blood coagulation parameters and platelet indices as potential predictors for the onset and severity of PE.Blood samples from 3 groups of subjects, normal pregnant women (n = 79, mild preeclampsia (mPE (n = 53 and severe preeclampsia (sPE (n = 42, were collected during early and late pregnancy. The levels of coagulative parameters and platelet indices were measured and compared among the groups. The receiver-operating characteristic (ROC curves of these indices were generated, and the area under the curve (AUC was calculated. The predictive values of the selected potential parameters were examined in binary regression analysis.During late pregnancy in the normal pregnancy group, the activated partial thromboplastin time (APTT, prothrombin time (PT, thrombin time (TT and platelet count decreased, while the fibrinogen level and mean platelet volume (MPV increased compared to early pregnancy (p<0.05. However, the PE patients presented with increased APTT, TT, MPV and D-dimer (DD during the third trimester. In the analysis of subjects with and without PE, TT showed the largest AUC (0.743 and high predictive value. In PE patients with different severities, MPV showed the largest AUC (0.671 and ideal predictive efficiency.Normal pregnancy causes a maternal physiological hypercoagulable state in late pregnancy. PE may trigger complex disorders in the endogenous coagulative pathways and consume platelets and FIB, subsequently activating thrombopoiesis and fibrinolysis. Thrombin time and MPV may serve as early monitoring markers for the onset and severity of PE

  13. Effect of genotype on haematology and biochemical parameters of ...

    African Journals Online (AJOL)

    Frizzle n = 33, Naked neck, n= 33 and Normal n = 33) were generated from 36 matured local chickens and used for the study to determine the effect of genotype on hematological and biochemical parameters of local chicken in the humid ...

  14. Influence of the Tabor parameter on the adhesive normal impact of spheres in Maugis-Dugdale approximation

    Science.gov (United States)

    Willert, E.; Lyashenko, I. A.; Popov, V. L.

    2017-10-01

    We study the influence of the adhesive interaction range on the coefficient of restitution in the normal impact of spheres by analytical and semi-analytical means based on a Maugis-Dugdale model for the adhesive stress. It is found, that—depending on the value of the Tabor parameter—the impact may start and end with and without direct contact between the surfaces. The hysteretic loss of energy takes its maximum value in the JKR-limit, which for even quite large values of the Tabor parameter noticeably overestimates this loss. Our findings can be applied for the rigorous study of adhesive collisions of elastic particles with and without friction.

  15. A preliminary study for investigating idiopatic normal pressure hydrocephalus by means of statistical parameters classification of intracranial pressure recordings.

    Science.gov (United States)

    Calisto, A; Bramanti, A; Galeano, M; Angileri, F; Campobello, G; Serrano, S; Azzerboni, B

    2009-01-01

    The objective of this study is to investigate Id-iopatic Normal Pressure Hydrocephalus (INPH) through a multidimensional and multiparameter analysis of statistical data obtained from accurate analysis of Intracranial Pressure (ICP) recordings. Such a study could permit to detect new factors, correlated with therapeutic response, which are able to validate a predicting significance for infusion test. The algorithm developed by the authors computes 13 ICP parameter trends on each of the recording, afterward 9 statistical information from each trend is determined. All data are transferred to the datamining software WEKA. According to the exploited feature-selection techniques, the WEKA has revealed that the most significant statistical parameter is the maximum of Single-Wave-Amplitude: setting a 27 mmHg threshold leads to over 90% of correct classification.

  16. Phytogenic additive on the diet of dairy heifers: Hematological parameters Parâmetros hematológicos de novilhas leiteiras submetidas a dietas com aditivos fitogênicos

    Directory of Open Access Journals (Sweden)

    Fernando Reimann Skonieski

    2009-12-01

    Full Text Available With objective to evaluate the effects of utilization of herbal extracts on supplementation of dairy heifers was conducted an experiment utilizing 12 Jersey’s heifers, with mean age and weight at start of trial of eight months old and 112 kg, respectively. The animals were separated in two groups, with same number of replications, considered a Control group (without addition of herbal extracts and a Treatment group (with inclusion of 500 grams of herbal extracts per ton. of ration. The herbal extracts utilized were a mixture of garlic, onion, cinnamon, clove and linseed. The animals received, daily, divided in two feeding, 2 kg. of ration and, approximately, 3 kg. of alfalfa hay and 10 kg. of chopped fresh black oat and ryegrass. The hematological parameters evaluated were red blood cell count (106 units/ L, hemoglobin and plasmatic protein (g/ dL, hematocrit (%, leukocytes, lymphocytes, neutrophils, and monocytes (units/ L. There were observed significantly differences to red blood cell count and monocytes count (P < 0.10, lymphocytes count (P < 0.05 and leukocytes count (P < 0.01. It is concluded what herbal extracts act above hematological parameters of dairy heifers receiving this supplement.Com o objetivo de avaliar os efeitos da utilização de extratos vegetais na suplementação de novilhas leiteiras, foi conduzido um experimento com 12 novilhas Jersey, com idade e peso médio de 8 meses e 112kg, respectivamente. Os animais foram separados em dois grupos, com o mesmo número de repetições, um chamado grupo-controle (sem a adição de extratos vegetais e um grupo-tratamento (com a inclusão de 500 gramas de extratos vegetais por tonelada de ração. Os extratos vegetais utilizados foram uma mistura comercial de alho, cebola, canela, cravo e linhaça. Os animais receberam, diariamente, divididos em duas refeições, 2kg de ração, 3kg de feno de alfafa e 10kg de aveia e azevém fresco. Os parâmetros hematológicos avaliados foram hem

  17. Selected hematological and immunological parameters in pigs ...

    African Journals Online (AJOL)

    Blood was collected from 64 healthy growing pigs one week before and one week after they were transferred to the finishing house. The following tests were performed: complete blood count with machine differential, immunoglobulin levels, C-reactive protein (CRP) level and cortisol level. Pigs were divided into two groups.

  18. The European Hematology Association Roadmap for European Hematology Research

    DEFF Research Database (Denmark)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke

    2016-01-01

    The European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European hematology...... research. Initiated by the EHA, around 300 experts contributed to the consensus document, which will help European policy makers, research funders, research organizations, researchers, and patient groups make better informed decisions on hematology research. It also aims to raise public awareness...... of the burden of blood disorders on European society, which purely in economic terms is estimated at €23 billion per year, a level of cost that is not matched in current European hematology research funding. In recent decades, hematology research has improved our fundamental understanding of the biology...

  19. Hematological changes after single large dose half-body irradiation

    International Nuclear Information System (INIS)

    Herrmann, T.; Friedrich, S.; Jochem, I.; Eberhardt, H.J.; Koch, R.; Knorr, A.

    1981-01-01

    The determination of different peripheral blood parameters aimed at the study of side effects on the hematological cellular system following a 5 - 8 Gy single large dose half-body irradiation in 20 patients. Compared to the initial values the leukocytes between the 6. and 14., the thrombocytes between the 14. and 21. postirradiation day as well as the lymphocytes between 3 hours and 4 weeks postirradiation were significantly decreased without exhibiting complications such as hemorrhages or infections. The hemoglobin, hematocrit and reticulocyte values revealed but a slight decrease normalized within a 28 days postirradiation period. Transfusions were necessary when a tumor-caused anemia was present prior to irradiation. Changes in serum activity of aminotransferases and lactate dehydrogenase occured during the first hours after irradiation and were due to enzyme release from destroyed tumor cells

  20. Association of Irisin Plasma Levels with Anthropometric Parameters in Children with Underweight, Normal Weight, Overweight, and Obesity

    Directory of Open Access Journals (Sweden)

    Leticia Elizondo-Montemayor

    2017-01-01

    Full Text Available The correlations between irisin levels, physical activity, and anthropometric measurements have been extensively described in adults with considerable controversy, but little evidence about these relationships has been found in children. The objective of this study is to correlate the plasma levels of irisin in underweight, normal weight, overweight, and obese children with anthropometric parameters and physical activity levels. A cross-sample of 40 children was divided into the following groups on the basis of body mass index (BMI percentile. The correlations of plasma irisin levels with physical activity, anthropometric, and metabolic measurements were determined. Plasma irisin levels (ng/mL were lower for the underweight group (164.2 ± 5.95 than for the normal weight and obese groups (182.8 ± 5.58; p<0.05. Irisin levels correlated positively with BMI percentile (0.387, waist circumference (0.373, and fat-free mass (0.353; p<0.05, but not with body muscle mass (−0.027. After a multiple linear regression analysis, only BMI percentile (0.564; p<0.008 showed a positive correlation with irisin. Our results indicated no association with metabolic parameters. A negative correlation with physical activity was observed. Interrelationships among body components might influence irisin levels in children.

  1. Changes of body fluid and hematology in toad and their rehabilitation following intermittent exposure to simulated high altitude

    Science.gov (United States)

    Biswas, H. M.; Boral, M. C.

    1986-06-01

    Three groups of adult male toads were exposed intermittently in a decompression chamber for a daily period of 4 and 8 hours at a time for 6 consecutive days to an “altitude” of 12,000; 18,000 and 24,000 feet (3658; 5486; 7315 m) respectively. Most of the exposed animals were sacrificed immediately after the last exposure, but only a few animals experiencing 8 hours of exposure were sacrificed after a further 16 hours of exposure at normal atmospheric pressure. Eight hours of daily exposure for 6 days causes a decrease of body fluids and an increase of hematological parameters in all the altitude exposed animals compared with to the changes noted in the animals having 4 hours of daily exposure for 6 days at the same altitude levels. The animals that were exposed to pressures equivalent to altitudes of 12,000 and 18,000 feet daily for 8 hours were found to return nearly to their normal body fluids and hematological balance after 16 hours of exposure to normal atmospheric pressure, whereas the animals exposed for a similar period at an equivalent 24,000 feet failed to get back their normal balance of body fluids and hematology after 16 hours of exposure at normal atmospheric pressure. The present experiment shows that the body weight loss and changes of body fluid and hematological parameters in the toad after exposure to simulated high altitude are due not only to dehydration, but suggest that hypoxia may also have a role.

  2. Determination of selenium toxicity to Oreochromis niloticus based on hematological parameters=Determinação da toxicidade do Selênio por meio de análises hematológicas em Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Silmara Regina Siqueira

    2012-04-01

    Full Text Available Selenium (Se is described as an essential micronutrient and participates in different biological functions, as the antioxidant defense systems maintenance and regulation. However, when in high concentrations, Se may cause toxic effects as well as hematological changes in fish. The aim of the present study was to determine the toxicity of selenium in the form of sodium selenate (Na2Se6+O4 in Oreochromis niloticus based on hematological parameters, after exposure to different concentrations (0.01, 0.14 and 1.4 mg Se6+ L-1. The erythrocytic and leukocytic series were examined over 14 days at intervals of 0, 3, 5, 7,10 and 14 days. The erythrocytic series showed significant alterations in the first 7 days, including the control group. Neutrophils and monocytes showed variations in the first 3 days at a concentration of 1.40 mgSe6+ L-1 characterizing an acute response. The total number of leukocytes was different in relation to time zero on all Se concentrations. The thrombocyte count also differed statistically from time zero and control in the first 3 days at 0.14 mgSe6+ L-1. These results indicate that different concentrations induce an acute response with diminution of total leukocytes, neutrophilia, monocytosis and thrombocytosis.O selênio (Se é descrito como um micronutriente essencial e participa de diversas funções biológicas. No entanto, quando em concentrações elevadas, o Se pode causar efeitos tóxicos, bem como alterações hematológicas em peixes. O objetivo do presente estudo foi determinar a toxicidade do selênio na forma de Selenato de sódio (Na2Se6+O4 em Oreochromis niloticus com base em parâmetros hematológicos, após a exposição a diferentes concentrações (0,01, 0,14 e 1,4 mg + Se6+ L-1. A série eritrocitária e leucocitária foram examinadas por 14 dias, em intervalos de 0, 3, 5, 7,10 e 14 dias. A série eritrocítica mostrou alterações significativas nos primeiros 7 dias, incluindo o grupo controle. Neutr

  3. Analysis of the diffusion tensor imaging parameters of a normal cervical spinal cord in a healthy population.

    Science.gov (United States)

    Wei, Liang-Feng; Wang, Shou-Sen; Zheng, Zhao-Cong; Tian, Jun; Xue, Liang

    2017-05-01

    Diffusion tensor imaging (DTI) shows great advantage in the diagnosis of brain diseases, including cervical spinal cord (CSC) disease. This study aims to obtain the normal values of the DTI parameters for a healthy population and to establish a baseline for CSC disease diagnosis using DTI. A total of 36 healthy adults were subjected to magnetic resonance imaging (MRI) for the entire CSC using the Siemens 3.0 T MR System. Sagittal DTI acquisition was carried out with a single-shot spin-echo echo-planar imaging (EPI) sequence along 12 non-collinear directions. Fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values were determined at different cervical levels using a region of interest (ROI) method, following which they were correlated with parameters, like age and sex. Further, diffusion tensor tracking (DTT) was carried out to reconstruct the white matter fiber bundles of the CSC. The full and complete fiber bundle structure of a normal CSC was confirmed in both the T2-weighted and DTI images. The FA and ADC values were significantly negatively correlated with each other and showed strongly negative and positive correlations with age, respectively, but not with sex. Additionally, there was no significant difference between the FA and the ADC values at different cervical levels. The DTI technique can act as an important supplement to the conventional MRI technique for CSC observation. Moreover, the FA and ADC values can be used as sensitive parameters in the DTI study on the CSC by taking the effects of age into consideration.

  4. Parâmetros clínicos, hematológicos, bioquímicos e hormonais de bovinos submetidos ao estresse calórico Clinical, hematological, biochemical, and hormonal parameters of cattle submitted to heat stress

    Directory of Open Access Journals (Sweden)

    F. Ferreira

    2009-08-01

    Full Text Available Caracterizaram-se as respostas clínicas, hematológicas, bioquímicas e hormonais de 71 bovinos F2 (½ Gir x ½ Holandês, 39 machos e 32 fêmeas, entre 14 e 20 meses de idade, submetidos a condições de termoneutralidade (22ºC a 70% de umidade relativa e ao estresse calórico (42ºC e 60% de umidade relativa. Os tratamentos foram realizados nas estações de verão e inverno em câmara bioclimática. Ao exame clínico, todos os animais apresentaram salivação e retração de globo ocular. Os animais apresentaram aumento do número de eritrócitos, concentração de hemoglobina, hematócrito, proteínas totais, ureia, creatinina, sódio, potássio, cloretos, cortisol, pH e densidade urinária e matéria seca das fezes no período de estresse calórico (P0,05. Concluiu-se que as variáveis hematológicas, bioquímicas e hormonais, bem como as características fenotípicas, revelaram-se importantes para caracterizar o estresse calórico em bovinos.Clinical, haemathological, biochemical, and hormonal parameters were characterized in 39 males and 32 females crossbred cattle (½ Holstein x ½ Gir, averaging 14 to 20 months of age, submitted to thermoneutrality (22ºC and 70% of RH and heat stress (42ºC and 60% RH in summer and winter in climatic chamber. At clinical examination, all animals presented salivation and ocular globe retraction. Under heat stress, animals increased erythrocytes count, hemoglobin total concentration, hematocrit, concentraction of total proteins, urea, creatinin, sodium, potassium, chlorides, cortisol, urinary pH and density, and dry matter of feces (P0.05. It was concluded that hematology, biochemical, and hormonal parameters are important to characterize the heat stress in cattle.

  5. Comparative analysis of morphological and topometric parameters of lumbar spine in normal state and in degenerative-dystrophic changes

    Directory of Open Access Journals (Sweden)

    Anisimova Е.А.

    2015-12-01

    Full Text Available Objective: to carry out comparative analysis and identify patterns of topographic variation patterns of lumbar spine in normal and degenerative changes. Material and methods. CT- and MRT-grams for men and women I (M1-22-35 years; W — 21-35 years and II (M2-36-60 years; W2-36-55 years periods of mature age with no signs of trauma, scoliosis and systemic diseases of the spine (n=140 and CT- and MRT-grams in patients with revealed degenerative changes in the lumbar spine degree II-III (n=120. The pictures with digital PACS system measure the height of the vertebral body, intervertebral disc height, vertical, horizontal diameter and the area of intervertebral foramen. Results. The height of the lumbar vertebral bodies normally increased from27,90±0,38mmatthe level of L, to 29,93±0,33 mm Lm, and then decreased to 24,35±0,27 mm at level L^, in osteochondrosis it is statistically significantly lower at all levels on average by 20%. The height of the intervertebral disc with osteochondrosis below at all levels by an average of 25% of its value in the range 5,27±0,19 to 6,13±0,17mm, while the normal disc height varies from 6,88±030 to 9,36±0,28mm. The area of intervertebral holes normally ranging from 103,29±5,78 to 127,99±5,92mm2, with osteochondrosis aperture area is reduced to a greater extent by decreasing the vertical diameter in comparison with the horizontal. Conclusion. For the studied parameters characteristic topographic variability has been determined. The maximum values parameters are marked at the top of the lumbar lordosis, at chest height, lumbar and lumbosacral junctions sizes are reduced. In osteochondrosis the intervertebral disc height and the height of lumbar vertebral bodies are reduced; intervertebral foramina area is also reduced to a greater extent by reducing the vertical diameter than the horizontal one.

  6. An iterative procedure for obtaining maximum-likelihood estimates of the parameters for a mixture of normal distributions

    Science.gov (United States)

    Peters, B. C., Jr.; Walker, H. F.

    1978-01-01

    This paper addresses the problem of obtaining numerically maximum-likelihood estimates of the parameters for a mixture of normal distributions. In recent literature, a certain successive-approximations procedure, based on the likelihood equations, was shown empirically to be effective in numerically approximating such maximum-likelihood estimates; however, the reliability of this procedure was not established theoretically. Here, we introduce a general iterative procedure, of the generalized steepest-ascent (deflected-gradient) type, which is just the procedure known in the literature when the step-size is taken to be 1. We show that, with probability 1 as the sample size grows large, this procedure converges locally to the strongly consistent maximum-likelihood estimate whenever the step-size lies between 0 and 2. We also show that the step-size which yields optimal local convergence rates for large samples is determined in a sense by the 'separation' of the component normal densities and is bounded below by a number between 1 and 2.

  7. An iterative procedure for obtaining maximum-likelihood estimates of the parameters for a mixture of normal distributions, 2

    Science.gov (United States)

    Peters, B. C., Jr.; Walker, H. F.

    1976-01-01

    The problem of obtaining numerically maximum likelihood estimates of the parameters for a mixture of normal distributions is addressed. In recent literature, a certain successive approximations procedure, based on the likelihood equations, is shown empirically to be effective in numerically approximating such maximum-likelihood estimates; however, the reliability of this procedure was not established theoretically. Here, a general iterative procedure is introduced, of the generalized steepest-ascent (deflected-gradient) type, which is just the procedure known in the literature when the step-size is taken to be 1. With probability 1 as the sample size grows large, it is shown that this procedure converges locally to the strongly consistent maximum-likelihood estimate whenever the step-size lies between 0 and 2. The step-size which yields optimal local convergence rates for large samples is determined in a sense by the separation of the component normal densities and is bounded below by a number between 1 and 2.

  8. Hematological effects of radiant heat-induced whole body hyperthermia on dogs.

    OpenAIRE

    Woods, J P; Schmitt-Tiggelaar, C L; d'Oleire, F; Rosenthal, R C; Robins, H I

    1996-01-01

    The effects on hematological parameters of radiant heat-induced whole body hyperthermia (WBH) at 40.5 degrees C and 41.8 degrees C were determined in 6 normal dogs. Complete blood counts determined prior to WBH, immediately post WBH plateau, and at 1, 2, 7, and 14 days posttreatment did not change significantly following WBH at 40.5 degrees C or 41.8 degrees C. Similarly, no significant changes were detected in platelet counts measured following 40.5 degrees C WBH. In contrast, platelet count...

  9. Multivariate relationships between international normalized ratio and vitamin K-dependent coagulation-derived parameters in normal healthy donors and oral anticoagulant therapy patients

    Directory of Open Access Journals (Sweden)

    Golanski Jacek

    2003-11-01

    Full Text Available Abstract Background and objectives International Normalized Ratio (INR is a world-wide routinely used factor in the monitoring of oral anticoagulation treatment (OAT. However, it was reported that other factors, e. g. factor II, may even better reflect therapeutic efficacy of OAT and, therefore, may be potentialy useful for OAT monitoring. The primary purpose of this study was to characterize the associations of INR with other vitamin K-dependent plasma proteins in a heterogenous group of individuals, including healthy donors, patients on OAT and patients not receiving OAT. The study aimed also at establishing the influence of co-morbid conditions (incl. accompanying diseases and co-medications (incl. different intensity of OAT on INR. Design and Methods Two hundred and three subjects were involved in the study. Of these, 35 were normal healthy donors (group I, 73 were patients on medication different than OAT (group II and 95 were patients on stable oral anticoagulant (acenocoumarol therapy lasting for at least half a year prior to the study. The values of INR and activated partial thromboplastin time (APTT ratio, as well as activities of FII, FVII, FX, protein C, and concentration of prothrombin F1+2 fragments and fibrinogen were obtained for all subjects. In statistical evaluation, the uni- and multivariate analyses were employed and the regression equations describing the obtained associations were estimated. Results Of the studied parameters, three (factors II, VII and X appeared as very strong modulators of INR, protein C and prothrombin fragments F1+2 had moderate influence, whereas both APTT ratio and fibrinogen had no significant impact on INR variability. Due to collinearity and low tolerance of independent variables included in the multiple regression models, we routinely employed a ridge multiple regression model which compromises the minimal number of independent variables with the maximal overall determination coefficient. The best

  10. determination of some haematological parameters in malaria

    African Journals Online (AJOL)

    USER

    2015-06-01

    ;66. Smita, C and Harish, C. (2013). Role of hematological parameters as an indicator of acute malaria infection in Uttarakhand State of. India. Mediterranean Journal of hematology and infectious diseases. Vol. 5, No 1. 83.

  11. Hematological and Biochemical Data Obtained in Rural Northern Uganda

    Directory of Open Access Journals (Sweden)

    Nirianne M. Q. Palacpac

    2014-05-01

    Full Text Available Reference intervals for common hematological and clinical chemistry parameters constitute an important basis for health care. Moreover, with increasing priority in drug and vaccine development for infectious diseases in Africa, the first priority is the safety evaluation and tolerability of the candidate interventions in healthy populations. To accurately assess health status and address adverse events, clinical reference intervals in the target population are necessary. We report on hematological and biochemical indices from healthy volunteers who participated in a clinical trial in Lira, northern Uganda. Median and nonparametric 95% percentiles on five hematology and 15 biochemistry analytes are shown. Although most hematological analytes conformed to reported reference intervals and trends in Africa, literature review from different African countries highlight the need for a region-specific children reference interval that can be appropriate for the population.

  12. Artificial intelligence in hematology.

    Science.gov (United States)

    Zini, Gina

    2005-10-01

    Artificial intelligence (AI) is a computer based science which aims to simulate human brain faculties using a computational system. A brief history of this new science goes from the creation of the first artificial neuron in 1943 to the first artificial neural network application to genetic algorithms. The potential for a similar technology in medicine has immediately been identified by scientists and researchers. The possibility to store and process all medical knowledge has made this technology very attractive to assist or even surpass clinicians in reaching a diagnosis. Applications of AI in medicine include devices applied to clinical diagnosis in neurology and cardiopulmonary diseases, as well as the use of expert or knowledge-based systems in routine clinical use for diagnosis, therapeutic management and for prognostic evaluation. Biological applications include genome sequencing or DNA gene expression microarrays, modeling gene networks, analysis and clustering of gene expression data, pattern recognition in DNA and proteins, protein structure prediction. In the field of hematology the first devices based on AI have been applied to the routine laboratory data management. New tools concern the differential diagnosis in specific diseases such as anemias, thalassemias and leukemias, based on neural networks trained with data from peripheral blood analysis. A revolution in cancer diagnosis, including the diagnosis of hematological malignancies, has been the introduction of the first microarray based and bioinformatic approach for molecular diagnosis: a systematic approach based on the monitoring of simultaneous expression of thousands of genes using DNA microarray, independently of previous biological knowledge, analysed using AI devices. Using gene profiling, the traditional diagnostic pathways move from clinical to molecular based diagnostic systems.

  13. A comparative study of some hematology and biochemical ...

    African Journals Online (AJOL)

    The effect of breed on serum biochemistry and hematological parameters of clinically healthy dogs was studied by collecting blood from the cephalic vein of ten (10) Alsatian and ten (10) local dogs. The parameters evaluated were not significantly different between the two breeds.

  14. Correlation of meteorological parameters and remotely sensed normalized difference vegetation index (NDVI) with cotton leaf curl virus (CLCV) in Multan

    International Nuclear Information System (INIS)

    Ahmed, A; Akhtar, A; Khalid, B; Shamim, A

    2013-01-01

    Climate change and weather has a profound effect on the spread of Cotton Leaf Curl Virus (CLCV) which is transmitted by whitefly. Climate change is altering temperature and precipitation patterns, resulting in the shift of some insect/pest from small population to large population thus effecting crops yield. To find out the relationship between the weather conditions, outburst of CLCV and changes in Normalized Difference Vegetation Index (NDVI) values due to the outburst of CLCV, a study was carried out for tehsil Multan. Data was acquired for the months of June, July, August and September for the year 2010. Regression analysis between CLCV and meteorological conditions as well as between CLCV and NDVI was performed. Meteorological parameters included temperature, humidity, precipitation, cloud cover, wind direction, pan evaporation and sunshine hours. NDVI values were calculated from SPOT satellite imagery (1km) using ArcMap10 and WinDisp v5.1. Correlation coefficients obtained in most of the cases were acceptable however the significance F and P-value were higher than their critical value at 95% level of significance. Therefore significant correlation was found only between CLCV and temperature and between CLCV and PAN evaporation during the month of July.

  15. Does diurnal variation affect the first trimester fetal aneuploidy screening test biochemical parameters of fetuses with normal nuchal translucency?

    Science.gov (United States)

    Karsli, Mehmet Fatih; Cakmak, Bulent; Seckin, Kerem Doga; Akkas Yilmaz, Elif; Akgul, Gurcan; Togrul, Cihan; Kucukozkan, Tuncay

    2016-01-01

    The purpose of this study was to investigate the effect of diurnal variation on biochemical results of first trimester aneuploidy screening test. A total of 2725 singleton pregnant female, who had normal fetal nuchal translucency (NT) thickness, were included in the study during this period. Individuals were divided into two groups according to the sampling time (morning group: 09:00-11:00 am and afternoon group: 02:00-04:00 pm). Hormonal parameters (free-beta human chorionic gonadotropin [free β-hCG] and pregnancy-associated plasma protein-A [PAPP-A] multiples of median [MoM] levels) of first trimester (11(+0)-13(+6) weeks) combined aneuploidy screening test were compared between morning and afternoon groups. PAPP-A MoM levels were significantly lower in the afternoon group when compared to the morning group (p = 0.001), whereas free β-hCG MoM levels were similar in the both groups (p = 0.392). Rate of high risk for Down syndrome (Combine risk >1/300) and amniocentesis ratio were found higher in the afternoon group than morning group, but there were no difference between groups for the number of fetuses with Down syndrome. Receiving the venous blood sample for first trimester aneuploidy screening test in the afternoon causes low PAPP-A MoM levels.

  16. Correlation of meteorological parameters and remotely sensed normalized difference vegetation index (NDVI) with cotton leaf curl virus (CLCV) in Multan

    Science.gov (United States)

    Ahmed, A.; Akhtar, A.; Khalid, B.; Shamim, A.

    2013-06-01

    Climate change and weather has a profound effect on the spread of Cotton Leaf Curl Virus (CLCV) which is transmitted by whitefly. Climate change is altering temperature and precipitation patterns, resulting in the shift of some insect/pest from small population to large population thus effecting crops yield. To find out the relationship between the weather conditions, outburst of CLCV and changes in Normalized Difference Vegetation Index (NDVI) values due to the outburst of CLCV, a study was carried out for tehsil Multan. Data was acquired for the months of June, July, August and September for the year 2010. Regression analysis between CLCV and meteorological conditions as well as between CLCV and NDVI was performed. Meteorological parameters included temperature, humidity, precipitation, cloud cover, wind direction, pan evaporation and sunshine hours. NDVI values were calculated from SPOT satellite imagery (1km) using ArcMap10 and WinDisp v5.1. Correlation coefficients obtained in most of the cases were acceptable however the significance F and P-value were higher than their critical value at 95% level of significance. Therefore significant correlation was found only between CLCV and temperature and between CLCV and PAN evaporation during the month of July.

  17. Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado Anticoagulant concentration, time and storage temperature on hematological parameters in automated blood count

    Directory of Open Access Journals (Sweden)

    Aécio Carlos de Oliveira

    2010-12-01

    Full Text Available O estudo teve como objetivo identificar os efeitos do tempo de estocagem, da temperatura de armazenamento e da quantidade de anticoagulante sobre parâmetros hematológicos de cães. Foram utilizadas amostras do sangue de dez cães de raças variadas, clinicamente hígidos. As alíquotas foram colhidas com 1,8mg; 3,6mg; 7,2mg e 14,4mg de ácido etilenodiaminotetracético (EDTA por mL de sangue, distribuídas em dois grupos: de 2°C a 8°C e temperatura ambiente. Após a coleta, foram avaliadas em quatro tempos: 0, 12, 24 e 48 horas. Usando um contador automático de células, foram avaliados leucócitos, eritrócitos, hemoglobina, hematócrito, volume corpuscular médio (VCM, índice de anisocitose eritrocitária (RDW, plaquetas e plaquetócrito (PCT. O valor do VCM diminuiu nas maiores concentrações de EDTA (7,2mg mL-1 e 14,4mg mL-1, com decréscimo de 2,36% na maior concentração. A temperatura e o tempo de armazenagem também ocasionaram alteração nesse parâmetro, ou seja, houve decréscimo no tempo 12 horas à temperatura de 2 a 8°C e aumento nos tempos 24 e 48 horas à temperatura ambiente (PThe study aimed to identify the effects of time, temperature of storage and excess of anticoagulant on hematological parameters of dogs. Blood samples of ten, clinically healthy dogs, of different breeds were utilized. Aliquots were stored with 1.8mg, 3.6mg, 7.2mg and 14.4mg of ethylenediaminetetraacetic acid (EDTA per mL of blood, divided into two groups: 2°C to 8°C and room temperature. Right after collection, they were evaluated in four times: 0h, 12h, 24h and 48h. White blood cells, red blood cells, hemoglobin, packed cell volume (PCV, mean corpuscular volume (MCV, red cell distribution width (RDW, platelet and thrombocrit (PCT were evaluated in the automatic cell counter. In the automatic cell counter analysis, the MCV increased significantly with higher concentrations of EDTA (7.2mg mL-1 and 14.4mg mL-1 peaking at 2.36%, in the highest

  18. Hematological characteristics of Delminichthys ghetaldii (Steindachner 1882 inhabiting the karst region of eastern Herzegovina

    Directory of Open Access Journals (Sweden)

    Dekić R.

    2014-01-01

    Full Text Available Hematological parameters are a valuable means of estimating the physiological status of fish and, indirectly, the state of their habitat. In order to use blood parameters as biomarkers, it is necessary to know their normal values and the referential intervals for a species. Such investigations are especially valuable in endemic and endangered fish species. In this study, parameters of erythrocyte lineage of Delminichthys ghetaldii (Steindachner 1882, an endemic species inhabiting the karst waters of eastern Herzegovina, were investigated. Fish were caught by nets in the region of Fatničko polje (Fatnica field after the water outflow in March 2013, blood was taken by heart puncture and erythrocyte parameters were determined. Their mean values were as follows: the number of erythrocyte (RBC = 1.474 x 1012/l; hemoglobin concentration (Hb = 72.50 g/l; packed cell volume (PCV = 0.398 l/l; mean corpuscular volume (MCV = 271.19 fl; mean corpuscular hemoglobin (MCH = 49.36 pg, and mean corpuscular hemoglobin concentration (MCHC = 193.16 g/l eryt. Body mass and morphometric features were also estimated and their mean values were: body mass = 25.49 g, total and standard body length = 14.13 cm and 11.98 cm, respectively, Fulton coefficient = 1.45. For the values of all parameters, the normality of distribution was tested, as well as differences between mean values of males and females. Pearson’s correlation and multiple regression analyses between mass, morphometric and hematological parameters have shown that erythrocyte parameters may influence body mass and of morphometric characteristics in this species.

  19. Estimating Non-Normal Latent Trait Distributions within Item Response Theory Using True and Estimated Item Parameters

    Science.gov (United States)

    Sass, D. A.; Schmitt, T. A.; Walker, C. M.

    2008-01-01

    Item response theory (IRT) procedures have been used extensively to study normal latent trait distributions and have been shown to perform well; however, less is known concerning the performance of IRT with non-normal latent trait distributions. This study investigated the degree of latent trait estimation error under normal and non-normal…

  20. Telomerase Activation in Hematological Malignancies

    Directory of Open Access Journals (Sweden)

    Joana Ropio

    2016-09-01

    Full Text Available Telomerase expression and telomere maintenance are critical for cell proliferation and survival, and they play important roles in development and cancer, including hematological malignancies. Transcriptional regulation of the rate-limiting subunit of human telomerase reverse transcriptase gen (hTERT is a complex process, and unveiling the mechanisms behind its reactivation is an important step for the development of diagnostic and therapeutic applications. Here, we review the main mechanisms of telomerase activation and the associated hematologic malignancies.

  1. 42 CFR 493.941 - Hematology (including routine hematology and coagulation).

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Hematology (including routine hematology and....941 Hematology (including routine hematology and coagulation). (a) Program content and frequency of challenge. To be approved for proficiency testing for hematology, a program must provide a minimum of five...

  2. Estimation of earthquake source parameters from GRACE observations of changes in Earth's gravitational potential field using normal modes

    Science.gov (United States)

    Sterenborg, G.; Simons, F. J.; Welch, E.; Morrow, E.; Mitrovica, J. X.

    2013-12-01

    Since its launch in 2002, the Gravity Recovery and Climate Experiment (GRACE) has yielded tremendous insights into the spatio-temporal changes of mass redistribution in the Earth system. Such changes occur on widely varying spatial and temporal scales and take place both on Earth's surface, e.g., atmospheric mass fluctuations and the exchange of water, snow and ice, as well as in its interior, e.g., glacial isostatic adjustment and earthquakes. Each of these processes causes changes in the Earth's gravitational potential field which GRACE observes. One example is the Antarctic and Greenland ice mass changes inferred from GRACE observations of the changing geopotential as well as the associated time rate of change of its degree 2 and 4 zonal harmonics observed by satellite laser ranging. Deforming the Earth's surface and interior both co- and post-seismically, with some of the deformation permanent, earthquakes can affect the geopotential at a spatial scale up to thousands of kilometers and at temporal scales from seconds to months. Traditional measurements of earthquakes, e.g., by seismometers, GPS and inSAR, observe the co- and post-seismic surface displacements and are invaluable in understanding earthquake triggering mechanisms, slip distributions, rupture dynamics and slow post-seismic changes. Space-based observations of geopotential changes can add a whole new dimension to this as such observations are also sensitive to changes in the Earth's interior, over a larger area affected by the earthquake, over longer timescales, beyond that of Earth's longest period normal mode, and because they have global sensitivity including over sparsely instrumented oceanic domains. We use a joint seismic and gravitational normal-mode formalism to quantify changes in the gravitational potential due to different types of earthquakes, comparing them to predictions from dislocation models. We discuss the inverse problem of estimating the source parameters of large earthquakes

  3. Relationship of tumor necrosis factor alpha genotypes with various biochemical parameters of normal, over weight and obese human subjects

    International Nuclear Information System (INIS)

    Raza, M.; Chaudhary, B.; Shakoori, A.R.

    2008-01-01

    Tumor Necrosis Factor (TNF-alpha) is expressed primarily in adipocytes and elevated levels of this cytokine have been associated with obesity. The purpose of this investigation was to test whether the TNF-alpha -308 polymorphism were associated with insulin resistance or obesity related traits in non-diabetic and diabetic patients visiting Sheikh Zayed Hospital, Lahore, Fatima Hospital and Irfan Clinic in Sargodha. In non diabetic subjects the AA allele carriers, compared with homozygous G allele carriers had significantly lower (28%) triglyceride values and 15% higher HDL yal ues, whereas other parameters tested 81id not show any significant variation. In diabetic patients the AA allele carriers, compared with GG allele carriers, besides having 31 % higher FBS and 26% higher creatinine, had 20% higher cholesterol and 34% higher triglycerides. The HDL values were 14% less, compared to GG allele carriers. In normal subjects (BMI 22.85:1:0.25 kgim2), the AA allele carriers showed 132%, 125%, 65% and 112% higher triglycerides, cholesterol and LDL values compared with GG allele carriers. The HDL and creatinine did not show any significant change. In the overweight subjects (BMI: 27.17+-0.17 kgim/sup 2/) all these values were lower than in AA allele carriers compared with GG allele carriers. The AA allele carries had FBS, triglycerides, cholesterol and LDL 28%, 48%, 14% and 14% lower than in the GG allele' carriers, respectively. In obese subjects, (BMI: 36.73+-0.78kgm/sup 2/), however, the FBS, triglycerides, cholesterol and creatinine values were 5%, 8%, 7% and 14% higher in AA allele carries compared to GG allele carriers, respectively. The LDL content was 8% lower in AA allele carrier as compared with the respective GG allele carriers, It is concluded that replacement of G at -308 with A leads to reduced risk for cardiovascular disease in non-diabetic subject, whereas in diabetic patients this mutation-increases the risk of CVD. Using BMI as index of obesity, it was

  4. Hematologic long-term modifications after radio-iodine therapy of the carcinoma of the thyroid gland. Pt. 1

    International Nuclear Information System (INIS)

    Schober, O.; Guenter, H.H.; Schwarzrock, R.; Hundeshagen, H.

    1987-01-01

    In a retrospective study, the hematologic parameters of 296 patients treated with iodine-131 (average: 536 mCi) for carcinoma of the thyroid gland were evaluated with respect to the different doses applied. The average observation period was 65 months. The most frequent hematologic modification was thrombocytopenia which was found in 35%, followed by erythrocytopenia in 24% of cases. The normal concentration of leucocytes was decreased in 11% and increased in 23% of cases, however, the medium value calculated for all patients showed a decrease of leucocytes by 7%. Pancytopenias were found in 4,4% of cases. The most severe decreases were presented after high doses of radio-iodine (≥ 1000 mCi). Pancytopenia may be considered as one of the causes of the death of three patients. (orig.) [de

  5. The European Hematology Association Roadmap for European Hematology Research : A consensus document

    NARCIS (Netherlands)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke; Coiffier, Bertrand; Cordonnier, Catherine; Döhner, Hartmut; de Wit, Thom Duyvené; Eichinger, Sabine; Fibbe, Willem; Green, Tony; de Haas, Fleur; Iolascon, Achille; Jaffredo, Thierry; Rodeghiero, Francesco; Salles, Gilles; Schuringa, Jan Jacob

    The European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European hematology

  6. Hematological Manifestation in HIV Infected Children

    International Nuclear Information System (INIS)

    Bhowmik, A.; Banerjee, P.

    2015-01-01

    Objective: To determine the common hematological abnormalities in HIV infected children and any association of these abnormalities with HIV disease severity. Study Design: Cross-sectional study. Place and Duration of Study: Regional Pediatric ART centre, Medical College and Hospital, Kolkata, West Bengal, India, from November 2011 to November 2012. Methodology: Children up to 12 years with confirmed diagnosis of HIV infection were clinically examined and tested for complete hemogram and CD4 count. Bone marrow study was done in selected patient depending on hemogram report. Children were divided in different stages according to WHO clinical staging. Each of the hematological parameters was assessed for any association with progression of disease. Fisher's Exact Test was used for determining the association between WHO clinical staging and abnormal blood parameters. P-value < 0.05 was taken as significant. Results: Sixty nine percent of the study population was anemic; 47.37% (18/38), 66.67% (8/12), 71.43% (15/21) and 93.10% (27/29) of stage 1, 2, 3 and 4 respectively were anemic in the study population (p=0.001). Leucopenia was present in 34% (34/100) children. Neutropenia and lymphopenia was present in 19% (19/100) and 22% (22/100) children. Lymphopenia was present in 7.89% (3/38), 16.67% (2/12), 19.05% (4/21) and 44.83% (13/29) of patient with stage 1, 2, 3 and 4 respectively (p=0.020). Eosinophilia was present in 17% (17/100) and thrombocytopenia in 11% (11/100) children. 2 patients with stage 4 disease were with hypoplastic bone marrow. Conclusion: Anemia was the most common hematological abnormality in HIV infected children. Anemia and lymphopenia had a significant association with the stage of the disease. (author)

  7. Hematology and immunology studies

    Science.gov (United States)

    Kimzey, S. L.

    1977-01-01

    A coordinated series of experiments were conducted to evaluate immunologic and hemotologic system responses of Skylab crewmen to prolonged space flights. A reduced PHA responsiveness was observed on recovery, together with a reduced number of T-cells, with both values returning to normal 3 to 5 days postflight. Subnormal red cell count, hemoglobin concentration, and hematocrit values also returned gradually to preflight limits. Most pronounced changes were found in the shape of red blood cells during extended space missions with a rapid reversal of these changes upon reentry into a normal gravitational environment.

  8. Plasma Concentration of Ascorbic Acid and Some Hematological ...

    African Journals Online (AJOL)

    hanumantp

    acid, which is a vital antioxidant, and blood cell production or morphology may be affected. Objective: To investigate the ... not adversely affect plasma ascorbic acid concentration and hematological parameters in adult humans. The study, however .... peroxidase/H202 halide system in vitro and in vivo. Am J Clin. Nutr 1995 ...

  9. 21 CFR 864.8625 - Hematology quality control mixture.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Hematology quality control mixture. 864.8625 Section 864.8625 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... parameters such as white cell count (WBC), red cell count (RBC), platelet count (PLT), hemoglobin, hematocrit...

  10. Eosinophilic Dermatosis of Hematologic Malignancy.

    Science.gov (United States)

    Lucas-Truyols, S; Rodrigo-Nicolás, B; Lloret-Ruiz, C; Quecedo-Estébanez, E

    Dermatosis characterized by tissue eosinophilia arising in the context of hematologic disease is known as eosinophilic dermatosis of hematologic malignancy. The most commonly associated malignancy is chronic lymphocytic leukemia. Eosinophilic dermatosis of hematologic malignancy is a rare condition with a wide variety of clinical presentations, ranging from papules, erythematous nodules, or blisters that simulate arthropod bites, to the formation of true plaques of differing sizes. Histology reveals the presence of abundant eosinophils. We present 4 new cases seen in Hospital Arnau de Vilanova, Valencia, during the past 7 years. Three of these cases were associated with chronic lymphocytic leukemia and 1 with mycosis fungoides. It is important to recognize this dermatosis as it can indicate progression of the underlying disease, as was the case in 3 of our patients. Copyright © 2017 AEDV. Publicado por Elsevier España, S.L.U. All rights reserved.

  11. Alterações nos parâmetros hematológicos de Gallus gallus domesticus experimentalmente infectados por Borrelia anserina Alterations in hematological parameters of Gallus gallus domesticus experimentally infected with Borrelia anserina

    Directory of Open Access Journals (Sweden)

    Raquel S. Lisbôa

    2008-10-01

    Full Text Available Borreliose aviária é uma doença septicêmica aguda, cosmopolita, que acomete diferentes espécies aviárias, sendo causada por Borrelia anserina Sakharoff, 1891. O objetivo do presente trabalho foi avaliar as alterações hematológicas em Gallus gallus domesticus experimentalmente infectados por B. anserina via vetor Argas (Persicargas miniatus. Um total de 27 aves da espécie G. g. domesticus com 67 dias de vida, foram divididas em três grupos inteiramente casualizados contendo nove animais cada. Um grupo foi exposto a carrapatos infectados por B. anserina (Grupo 1; outro a carrapatos livres deste agente (Grupo 2; além de um grupo não exposto aos carrapatos (Grupo 3. Realizaram-se esfregaços sangüíneos diariamente, a partir do primeiro dia de exposição ao vetor, até o 25º dia pós-exposição (DPE. Amostras de sangue foram coletadas 3 dias antes da exposição aos carrapatos, e 3, 8 e 18 dias pós-exposição (DPE, para a realização dos hemogramas. O exame dos esfregaços das aves do Grupo 1 revelou grande número de espiroquetas. Os esfregaços sangüíneos dos Grupos 2 e 3 mantiveram-se negativos durante todo o período experimental. De acordo com os resultados das avaliações hematológicas, as aves do Grupo 1 apresentaram um quadro de anemia normocítica normocrômica em oito DPE, além de leucocitose com heterofilia e monocitose que cursaram paralelamente ao período de espiroquetemia. Concluiu-se que a infecção por B. anserina determinou nas aves do Grupo 1 alterações hematológicas compatíveis com uma infecção bacteriana de moderada gravidade, evoluindo para auto-cura, nas condições experimentais estabelecidas neste trabalho.Avian spirochaetosis is a cosmopolite acute septicemic disease of many avian species, caused by Borrelia anserina Sakharoff, 1891. The present study assesses the estimate of the hematological alterations of Gallus gallus domesticus experimentally infected with B. anserina by vector Argas

  12. Extracellular Vesicles in Hematological Disorders

    Directory of Open Access Journals (Sweden)

    Anat Aharon

    2014-10-01

    Full Text Available Extracellular vesicles (EVs, comprised of exosomes, microparticles, apoptotic bodies, and other microvesicles, are shed from a variety of cells upon cell activation or apoptosis. EVs promote clot formation, mediate pro-inflammatory processes, transfer proteins and miRNA to cells, and induce cell signaling that regulates cell differentiation, proliferation, migration, invasion, and apoptosis. This paper will review the contribution of EVs in hematological disorders, including hemoglobinopathies (sickle cell disease, thalassemia, paroxysmal nocturnal hemoglobinuria, and hematological malignancies (lymphomas, myelomas, and acute and chronic leukemias.

  13. On Better Estimating and Normalizing the Relationship between Clinical Parameters: Comparing Respiratory Modulations in the Photoplethysmogram and Blood Pressure Signal (DPOP versus PPV

    Directory of Open Access Journals (Sweden)

    Paul S. Addison

    2015-01-01

    Full Text Available DPOP (ΔPOP or Delta-POP is a noninvasive parameter which measures the strength of respiratory modulations present in the pulse oximeter waveform. It has been proposed as a noninvasive alternative to pulse pressure variation (PPV used in the prediction of the response to volume expansion in hypovolemic patients. We considered a number of simple techniques for better determining the underlying relationship between the two parameters. It was shown numerically that baseline-induced signal errors were asymmetric in nature, which corresponded to observation, and we proposed a method which combines a least-median-of-squares estimator with the requirement that the relationship passes through the origin (the LMSO method. We further developed a method of normalization of the parameters through rescaling DPOP using the inverse gradient of the linear fitted relationship. We propose that this normalization method (LMSO-N is applicable to the matching of a wide range of clinical parameters. It is also generally applicable to the self-normalizing of parameters whose behaviour may change slightly due to algorithmic improvements.

  14. On better estimating and normalizing the relationship between clinical parameters: comparing respiratory modulations in the photoplethysmogram and blood pressure signal (DPOP versus PPV).

    Science.gov (United States)

    Addison, Paul S; Wang, Rui; Uribe, Alberto A; Bergese, Sergio D

    2015-01-01

    DPOP (ΔPOP or Delta-POP) is a noninvasive parameter which measures the strength of respiratory modulations present in the pulse oximeter waveform. It has been proposed as a noninvasive alternative to pulse pressure variation (PPV) used in the prediction of the response to volume expansion in hypovolemic patients. We considered a number of simple techniques for better determining the underlying relationship between the two parameters. It was shown numerically that baseline-induced signal errors were asymmetric in nature, which corresponded to observation, and we proposed a method which combines a least-median-of-squares estimator with the requirement that the relationship passes through the origin (the LMSO method). We further developed a method of normalization of the parameters through rescaling DPOP using the inverse gradient of the linear fitted relationship. We propose that this normalization method (LMSO-N) is applicable to the matching of a wide range of clinical parameters. It is also generally applicable to the self-normalizing of parameters whose behaviour may change slightly due to algorithmic improvements.

  15. American Society of Pediatric Hematology/Oncology

    Science.gov (United States)

    ... Conference Learn More Explore career opportunities in pediatric hematology/oncology Visit the ASPHO Career Center. Learn More ... Use & Privacy Policy » © The American Society of Pediatric Hematology/Oncology

  16. Hematologic outcomes after total splenectomy and partial splenectomy for congenital hemolytic anemia.

    Science.gov (United States)

    Englum, Brian R; Rothman, Jennifer; Leonard, Sarah; Reiter, Audra; Thornburg, Courtney; Brindle, Mary; Wright, Nicola; Heeney, Matthew M; Jason Smithers, C; Brown, Rebeccah L; Kalfa, Theodosia; Langer, Jacob C; Cada, Michaela; Oldham, Keith T; Scott, J Paul; St Peter, Shawn D; Sharma, Mukta; Davidoff, Andrew M; Nottage, Kerri; Bernabe, Kathryn; Wilson, David B; Dutta, Sanjeev; Glader, Bertil; Crary, Shelley E; Dassinger, Melvin S; Dunbar, Levette; Islam, Saleem; Kumar, Manjusha; Rescorla, Fred; Bruch, Steve; Campbell, Andrew; Austin, Mary; Sidonio, Robert; Blakely, Martin L; Rice, Henry E

    2016-01-01

    The purpose of this study was to define the hematologic response to total splenectomy (TS) or partial splenectomy (PS) in children with hereditary spherocytosis (HS) or sickle cell disease (SCD). The Splenectomy in Congenital Hemolytic Anemia (SICHA) consortium registry collected hematologic outcomes of children with CHA undergoing TS or PS to 1 year after surgery. Using random effects mixed modeling, we evaluated the association of operative type with change in hemoglobin, reticulocyte counts, and bilirubin. We also compared laparoscopic to open splenectomy. The analysis included 130 children, with 62.3% (n=81) undergoing TS. For children with HS, all hematologic measures improved after TS, including a 4.1g/dl increase in hemoglobin. Hematologic parameters also improved after PS, although the response was less robust (hemoglobin increase 2.4 g/dl, p<0.001). For children with SCD, there was no change in hemoglobin. Laparoscopy was not associated with differences in hematologic outcomes compared to open. TS and laparoscopy were associated with shorter length of stay. Children with HS have an excellent hematologic response after TS or PS, although the hematologic response is more robust following TS. Children with SCD have smaller changes in their hematologic parameters. These data offer guidance to families and clinicians considering TS or PS. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Hematologic outcomes after total splenectomy and partial splenectomy for congenital hemolytic anemia☆☆☆

    Science.gov (United States)

    Englum, Brian R.; Rothman, Jennifer; Leonard, Sarah; Reiter, Audra; Thornburg, Courtney; Brindle, Mary; Wright, Nicola; Heeney, Matthew M.; Smithers, C. Jason; Brown, Rebeccah L.; Kalfa, Theodosia; Langer, Jacob C.; Cada, Michaela; Oldham, Keith T.; Scott, J. Paul; St Peter, Shawn D; Sharma, Mukta; Davidoff, Andrew M.; Nottage, Kerri; Bernabe, Kathryn; Wilson, David B.; Dutta, Sanjeev; Glader, Bertil; Crary, Shelley E.; Dassinger, Melvin S.; Dunbar, Levette; Islam, Saleem; Kumar, Manjusha; Rescorla, Fred; Bruch, Steve; Campbell, Andrew; Austin, Mary; Sidonio, Robert; Blakely, Martin L.; Rice, Henry E.

    2016-01-01

    Purpose The purpose of this study was to define the hematologic response to total splenectomy (TS) or partial splenectomy (PS) in children with hereditary spherocytosis (HS) or sickle cell disease (SCD). Methods The Splenectomy in Congenital Hemolytic Anemia (SICHA) consortium registry collected hematologic outcomes of children with CHA undergoing TS or PS to 1 year after surgery. Using random effects mixed modeling, we evaluated the association of operative type with change in hemoglobin, reticulocyte counts, and bilirubin. We also compared laparoscopic to open splenectomy. Results The analysis included 130 children, with 62.3% (n = 81) undergoing TS. For children with HS, all hematologic measures improved after TS, including a 4.1 g/dl increase in hemoglobin. Hematologic parameters also improved after PS, although the response was less robust (hemoglobin increase 2.4 g/dl, p < 0.001). For children with SCD, there was no change in hemoglobin. Laparoscopy was not associated with differences in hematologic outcomes compared to open. TS and laparoscopy were associated with shorter length of stay. Conclusion Children with HS have an excellent hematologic response after TS or PS, although the hematologic response is more robust following TS. Children with SCD have smaller changes in their hematologic parameters. These data offer guidance to families and clinicians considering TS or PS. PMID:26613837

  18. HEMATOLOGIC CHANGES FOLLOWING SIMPLE EXODONTIA,

    Science.gov (United States)

    Hematologic studies were carried out on 90 systemically healthy, young adult males, some of whom underwent a simple exodontic experience. In one...after exodontia . In the 4-hour study significant changes following extraction were noted for white blood cells, neutrophils, lymphocytes, hematocrit

  19. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    Science.gov (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver. Copyright © 2015 Elsevier GmbH. All rights reserved.

  20. Comparing the Effectiveness of Dietary Vitamin C and Exercise Interventions on Fertility Parameters in Normal Obese Men.

    Science.gov (United States)

    Rafiee, Bahare; Morowvat, Mohammad Hossein; Rahimi-Ghalati, Nasrin

    2016-04-16

    Comparing the effectiveness of dietary vitamin C and weight loss exercises interventions for weight loss on semen characteristics in normal obese man. A total number of 200 men were randomly allocated into two groups based on body mass index, exercise and vitamin C groups. Also, 50 men with normal spermogram were placed in a control group. In exercise group, a 6 months intensive exercise program was designed under a coach's supervision to reduce the body weight. In vitamin C group, 1,000 mg of vitamin C were given every other day as supplement. Weight loss increased the volume of semen in participants with 25-30 (P = .02) and more than 30 body mass index (P = .001). The increased concentration of sperm per mL of semen in body mass index (BMI) 25-30 group (P = .01) and more than 30 (P = .003) BMI was significant. Improving sperm motility after two hours in participants with more than 30 (P = .01) BMI was significant. In vitamin C group, the improvement of sperm concentration in participants who had less than 25 (P = .01), between 25 and 30 (P = .01), more than 30 (P = .02) BMI was significant. Sperm motility improved in all three groups (P = .001, P = .02 and P = .003, respectively). Weight loss can significantly increase semen volume, its concentration, its mobility and percentage of normal morphology. Consuming vitamin C significantly improves sperm concentration and mobility, but the semen volume and the percentage of normal morphology will not change significantly.

  1. 42 CFR 493.1215 - Condition: Hematology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Hematology. 493.1215 Section 493.1215 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES....1215 Condition: Hematology. If the laboratory provides services in the specialty of Hematology, the...

  2. 42 CFR 493.849 - Condition: Hematology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Hematology. 493.849 Section 493.849 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... These Tests § 493.849 Condition: Hematology. The specialty of hematology, for the purpose of proficiency...

  3. Multiple linear regression approach for the analysis of the relationships between joints mobility and regional pressure-based parameters in the normal-arched foot.

    Science.gov (United States)

    Caravaggi, Paolo; Leardini, Alberto; Giacomozzi, Claudia

    2016-10-03

    Plantar load can be considered as a measure of the foot ability to transmit forces at the foot/ground, or foot/footwear interface during ambulatory activities via the lower limb kinematic chain. While morphological and functional measures have been shown to be correlated with plantar load, no exhaustive data are currently available on the possible relationships between range of motion of foot joints and plantar load regional parameters. Joints' kinematics from a validated multi-segmental foot model were recorded together with plantar pressure parameters in 21 normal-arched healthy subjects during three barefoot walking trials. Plantar pressure maps were divided into six anatomically-based regions of interest associated to corresponding foot segments. A stepwise multiple regression analysis was performed to determine the relationships between pressure-based parameters, joints range of motion and normalized walking speed (speed/subject height). Sagittal- and frontal-plane joint motion were those most correlated to plantar load. Foot joints' range of motion and normalized walking speed explained between 6% and 43% of the model variance (adjusted R 2 ) for pressure-based parameters. In general, those joints' presenting lower mobility during stance were associated to lower vertical force at forefoot and to larger mean and peak pressure at hindfoot and forefoot. Normalized walking speed was always positively correlated to mean and peak pressure at hindfoot and forefoot. While a large variance in plantar pressure data is still not accounted for by the present models, this study provides statistical corroboration of the close relationship between joint mobility and plantar pressure during stance in the normal healthy foot. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Precise measurement of renal filtration and vascular parameters using a two-compartment model for dynamic contrast-enhanced MRI of the kidney gives realistic normal values

    International Nuclear Information System (INIS)

    Tofts, Paul S.; Cutajar, Marica; Mendichovszky, Iosif A.; Peters, A.M.; Gordon, Isky

    2012-01-01

    To model the uptake phase of T 1 -weighted DCE-MRI data in normal kidneys and to demonstrate that the fitted physiological parameters correlate with published normal values. The model incorporates delay and broadening of the arterial vascular peak as it appears in the capillary bed, two distinct compartments for renal intravascular and extravascular Gd tracer, and uses a small-vessel haematocrit value of 24%. Four physiological parameters can be estimated: regional filtration K trans (ml min -1 [ml tissue ] -1 ), perfusion F (ml min -1 [100 ml tissue ] -1 ), blood volume v b (%) and mean residence time MRT (s). From these are found the filtration fraction (FF; %) and total GFR (ml min -1 ). Fifteen healthy volunteers were imaged twice using oblique coronal slices every 2.5 s to determine the reproducibility. Using parenchymal ROIs, group mean values for renal biomarkers all agreed with published values: K trans : 0.25; F: 219; v b : 34; MRT: 5.5; FF: 15; GFR: 115. Nominally cortical ROIs consistently underestimated total filtration (by ∝ 50%). Reproducibility was 7-18%. Sensitivity analysis showed that these fitted parameters are most vulnerable to errors in the fixed parameters kidney T 1 , flip angle, haematocrit and relaxivity. These renal biomarkers can potentially measure renal physiology in diagnosis and treatment. circle Dynamic contrast-enhanced magnetic resonance imaging can measure renal function. circle Filtration and perfusion values in healthy volunteers agree with published normal values. circle Precision measured in healthy volunteers is between 7 and 15%. (orig.)

  5. BMC Blood Disorders becomes BMC Hematology: evolving along with the hematology field

    OpenAIRE

    Chap, Christna

    2013-01-01

    This Editorial marks the launch of BMC Hematology, formerly known as BMC Blood Disorders, within the BMC series of journals published by BioMed Central. The scope of BMC Hematology encompasses basic, experimental and clinical research related to hematology. In this Editorial we will discuss the rationale behind this relaunch and how, as an open access journal providing unrestricted and free access to scientific and scholarly work, BMC Hematology will help disseminate research in the hematolog...

  6. Influence of hydration status in normal subjects. Fractional analysis of parameters of Tc-99m DTPA and Tc-99m MAG3 renography

    International Nuclear Information System (INIS)

    Jung, Hyun Suk; Chung, Yong An; Kim, Eui Neyng; Kim, Sung Hoon; Lee, Sung Yong; Sohn, Hyung Sun; Park, Young Ha; Chung, Soo Kyo

    2005-01-01

    The purpose of this study was to evaluate the influence of hydration status upon renogram patterns and renal physiological parameters and clarify the differences between DTPA and MAG 3 studies in normal volunteers. The study populations were 22 kidneys of 11 volunteers with no history of hypertension or renal disease with normal serum creatinine levels. They were 6 men and 5 women aged from 24 to 48 yrs (mean age: 33.4 yrs). Renal scintigraphies with both 185 MBq (5 mCi) of Tc-99m DTPA and Tc-99m MAG 3 were performed after dehydration (urine specific gravity >1.025) and adequate hydration (urine specific gravity 3 with the whole kidney and cortical ROIs. Dehydration may bring about a false positive curve pattern on renograms which can be prevented or minimized by using the cortical ROI. There were insignificant differences between most parameters of Tc-99m DTPA and Tc-99m MAG 3 . (author)

  7. Comparison of malnutrition inflammation score, anthropometry and biochemical parameters in assessing the difference in protein-energy wasting between normal weight and obese patients undergoing haemodialysis.

    Science.gov (United States)

    Alipoor, Elham; Hosseinzadeh-Attar, Mohammad Javad; Mahdavi-Mazdeh, Mitra; Yaseri, Mehdi; Zahed, Narges S

    2017-07-01

    Protein-energy wasting (PEW) is prevalent in haemodialysis. Obesity is an independent risk factor of kidney insufficiency, but it is proposed to have beneficial roles in better outcomes in the final stage of disease. Better nutritional status and body reserves are among probable mechanisms, but direct examinations are limited. The present study aimed to investigate whether obese patients have preferable nutritional status compared to normal weight patients based on malnutrition inflammation score (MIS) and other PEW parameters in haemodialysis. This case-control study investigated 52 normal weight (18.5 < body mass index (BMI) < 25 kg/m 2 ) and 48 obese (BMI≥30 kg/m 2 ) patients on regular haemodialysis. PEW was assessed based on anthropometric and biochemical factors, recent weight changes, appetite, anorexia, dietary intake and MIS. Obese patients had better MIS compared with the normal weight group (P < 0.001), although varying degrees of wasting were prevalent among this group too (75% mild and 25% moderate wasting). The obese group had less significant weight loss (4.2 vs 8%) and anorexia and better appetite. However, a considerable percentage of patients in both groups showed muscle (94.6% of normal weight and 19.5% of obese) and peripheral fat tissue (89.2% of normal weight and 31.7% of obese) losses compared to the 50th percentile. Biochemical parameters were not significantly different between groups except for triglyceride (P = 0.001), transferrin and total iron-binding capacity (P = 0.028). MIS was significantly better in obese patients; however, both groups showed degrees of wasting based on MIS and other PEW parameters. Nutritional status of obese haemodialysis patients should be monitored regularly because of high risk of PEW like other BMI categories. © 2017 Dietitians Association of Australia.

  8. Usability of a theory of visual attention (TVA) for parameter-based measurement of attention I: evidence from normal subjects

    DEFF Research Database (Denmark)

    Finke, Kathrin; Bublak, Peter; Krummenacher, Joseph

    2005-01-01

    The present study investigated the usability of whole and partial report of briefly displayed letter arrays as a diagnostic tool for the assessment of attentional functions. The tool is based on Bundesen's (1990, 1998, 2002; Bundesen et al., 2005) theory of visual attention (TVA), which assumes...... four separable attentional components: processing speed, working memory storage capacity, spatial distribution of attention, and top-down control. A number of studies (Duncan et al., 1999; Habekost & Bundesen, 2003; Peers et al., 2005) have already demonstrated the clinical relevance...... clinical tests measuring similar constructs. The empirical independence of the four TVA parameters is suggested by nonsignificant or, in the case of processing speed and working memory storage capacity, only modest correlations between the parameter values....

  9. Usability of a theory of visual attention (TVA) for parameter-based measurement of attention I: evidence from normal subjects

    DEFF Research Database (Denmark)

    Finke, Kathrin; Bublak, Peter; Krummenacher, Joseph

    2005-01-01

    The present study investigated the usability of whole and partial report of briefly displayed letter arrays as a diagnostic tool for the assessment of attentional functions. The tool is based on Bundesen's (1990, 1998, 2002; Bundesen et al., 2005) theory of visual attention (TVA), which assumes...... four separable attentional components: processing speed, working memory storage capacity, spatial distribution of attention, and top-down control. A number of studies (Duncan et al., 1999; Habekost & Bundesen, 2003; Peers et al., 2005) have already demonstrated the clinical relevance...... of these parameters. The present study was designed to examine whether (a) a shortened procedure bears sufficient accuracy and reliability, (b) whether the procedures reveal attentional constructs with clinical relevance, and (c) whether the mathematically independent parameters are also empirically independent...

  10. Normal Morning MCH Levels and No Association with REM or NREM Sleep Parameters in Narcolepsy Type 1 and Type 2

    DEFF Research Database (Denmark)

    Schrölkamp, Maren; Jennum, Poul J; Gammeltoft, Steen

    2017-01-01

    STUDY OBJECTIVES: Other than hypocretin-1 (HCRT-1) deficiency in narcolepsy type 1 (NT1), the neurochemical imbalance of NT1 and narcolepsy type 2 (NT2) with normal HCRT-1 levels is largely unknown. The neuropeptide melanin-concentrating hormone (MCH) is mainly secreted during sleep and is involved...... in rapid eye movement (REM) and nonrapid eye movement (NREM) sleep regulation. Hypocretin neurons reciprocally interact with MCH neurons. We hypothesized that altered MCH secretion contributes to the symptoms and sleep abnormalities of narcolepsy and that this is reflected in morning cerebrospinal fluid...

  11. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  12. Parâmetros hematológicos do roedor fossorial Ctenomys lami (Rodentia, Ctenomidae no estado do Rio Grande do Sul Hematological parameters of fossorial rodent Ctenomys lami (Rodentia, Ctenomidae in Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Gisele G. Stein

    2010-08-01

    Full Text Available O perfil hematológico sanguíneo foi determinado em três populações de Ctenomys lami, em áreas denominadas A e B, impactadas pela bovinocultura, e C, sem impacto antrópico, ambas no sul do Brasil. Sessenta e dois animais foram coletados ao total. Os valores de hematócrito (Ht, hemoglobina (Hb e eritrócitos apresentaram diferenças significativas entre machos e fêmeas. Os valores médios de Ht e a Hb encontrados na espécie foram mais baixos em comparação com os de outras espécies de roedores subterrâneos, podendo estes valores estarem relacionados ao habitat de forrageio ou às características do solo. Também foram encontradas diferenças significativas nas médias de hemoglobina, CHCM e linfócitos em animais das áreas A e B em relação à área C. O valor da média dos hematócritos dos animais entre as áreas foi mais elevado nas áreas A e B, porém significativamente diferentes entre A e C. Algumas dessas alterações sugerem a relação dos valores encontrados com o estresse dos animais em relação a áreas impactadas. Variações significativas no VCM foram encontradas entre os animais das áreas A e C, e também nas plaquetas destes entre as áreas A e B. Não foram observados Corpúsculo de Kurloff no sangue dos animais analisados. Os valores hematológicos encontrados nesses espécimes de Ctenomys lami fornecem informações importantes sobre a espécie e podem ser úteis em outras pesquisas.The hematological profile was determined in three populations of Ctenomys lami that inhabits three different areas nominated as A and B, affected by cattle production, and C, without human impact, all of them in southern Brazil, under the same geologic formation. Sixty two individuals were collected. The packed cell volume (PCV values, hemoglobin (Hb and red blood cell (RBC count presents statistic significant differences between males and females. PCV and Hb values were lower in comparison with other subterranean rodents, which

  13. Relationship between cathepsin B activity and compositional parameters in dry-cured hams of normal and defective texture.

    Science.gov (United States)

    Parolari, G; Virgili, R; Schivazappa, C

    1994-01-01

    Thirty-eight Italian dry-cured hams were analysed for cathepsin B activity, proximate composition and proteolysis index and results were related to lean tissue texture, as assessed by an expert panel, in order to search for relationships between excessive softness, a major problem in the raw ham industry, and chemical parameters. Softness was found to be related to protein breakdown which, in turn, was linked with higher residual cathepsin B activity and, to a lesser extent, to lower salt content. Results suggest that the use of raw ham of controlled enzyme activity would improve the texture quality of the end product. Copyright © 1994. Published by Elsevier Ltd.

  14. Acute hematologic emergencies in oncology

    International Nuclear Information System (INIS)

    Kristof, L.

    2012-01-01

    Malignant disease and its treatment are often being complicated by development of serious and at times life-threatening emergencies. Early recognition and treatment of these acute events are important to reduce morbidity and mortality in cancer patients. The following article provides an overview of several hematologic emergencies, which occur due abnormal hemopoiesis (e.g. hyperleukocytosis, anemia, thrombocytopenia), abnormal hemo stasis (e.g. hemorrhage, pulmonary embolism, disseminated intravascular coagulation), or are related to blood products transfusions (transfuse reactions). (author)

  15. Pharmacodynamic analysis of hematologic profiles

    Energy Technology Data Exchange (ETDEWEB)

    Rosner, G.L. [Univ. Medical Center, Durham, NC (United States); Mueller, P. [Duke Univ., Durham, NC (United States)

    1994-12-01

    We discuss the analysis of the myelosuppressive effects of chemotherapy. Such analyses examine hematologic data that arise by monitoring patients after treatment with high doses of chemotherapy. We propose a flexible approach for modeling such information and, using data collected as part of a Phase I study of an anticancer agent, show some interesting aspects of the data that become available after fitting models this way. 29 refs., 13 figs., 2 tabs.

  16. Model, parameter and code of environmental dispersion of gaseous effluent under normal operation from nuclear power plant with 600 MWe

    International Nuclear Information System (INIS)

    Hu Erbang; Gao Zhanrong

    1998-06-01

    The model of environmental dispersion of gaseous effluence under normal operation from a nuclear power plant with 600 MWe is established to give a mathematical expression of annual mean atmospheric dispersion factor under mixing release condition based on quality assessment of radiological environment for 30 years of Chinese nuclear industry. In calculation, the impact from calm and other following factors have been taken into account: mixing layer, dry and wet deposition, radioactive decay and buildings. The doses caused from the following exposure pathways are also given by this model: external exposure from immersion cloud and ground deposition, internal exposure due to inhalation and ingestion. The code is named as ROULEA. It contains four modules, i.e. INPUT, ANRTRI, CHIQV and DOSE for calculating 4-dimension joint frequency, annual mean atmospheric dispersion factor and doses

  17. Hematological malignancies in Al-amal oncology unit, aden.

    Science.gov (United States)

    Al-Kahiry, Waiel

    2012-03-01

    The hematological malignancies (HM) are group of neoplasms that arise through malignant transformation of bone marrow derived cells. The great diversity seen in this group of disorders is a reflection of the complexity of normal hematopoiesis and the immune system. In the current study, the author retrospectively studied HM patients from 2008 to 2010, and compared with prevalence of solid tumor, and found HM represented one-fifth of all malignancies managed in the Oncology Unit, and lymphomas were the commonest HM.

  18. Diclocor is superior to diclofenac sodium and quercetin in normalizing biochemical parameters in rats with collagen-induced osteoarthritis.

    Science.gov (United States)

    Zupanets, I A; Shebeko, S K; Popov, O S; Shalamay, A S

    2016-02-01

    The aim of the present study was to investigate anti-inflammatory activity of Diclocor in the setting of collagen-induced osteoarthritis in rats in comparison with its active monocomponents-diclofenac sodium and quercetin. The study was conducted on the model of collagen-induced osteoarthritis and included measurement of sialic acids, glycoproteins, C-reactive protein, prostaglandin E2, 6-keto-prostaglandin F1α, thromboxane B2, and leukotriene B4. Lastly, morphologic study with morphometry was also performed. Diclocor is superior to quercetin and diclofenac sodium by the degree of pharmacological effect on some of the studied parameters. The differences between the values were statistically significant. Diclocor is a promising corrector of inflammatory and destructive joint diseases. Owing to the presence of both diclofenac sodium and quercetin in its composition, Diclocor exhibits a complex mechanism of anti-inflammatory action affecting cyclooxygenase and lipoxygenase ways of arachidonic acid biotransformation.

  19. Precise measurement of renal filtration and vascular parameters using a two-compartment model for dynamic contrast-enhanced MRI of the kidney gives realistic normal values

    Energy Technology Data Exchange (ETDEWEB)

    Tofts, Paul S. [Brighton and Sussex Medical School, Falmer, Sussex (United Kingdom); UCL Institute of Neurology, London (United Kingdom); Cutajar, Marica [Brighton and Sussex Medical School, Falmer, Sussex (United Kingdom); UCL Institute of Child Health, London (United Kingdom); Mendichovszky, Iosif A. [University of Manchester, Imaging Science and Biomedical Engineering, Manchester (United Kingdom); Peters, A.M. [Brighton and Sussex Medical School, Falmer, Sussex (United Kingdom); Gordon, Isky [UCL Institute of Child Health, London (United Kingdom)

    2012-06-15

    To model the uptake phase of T{sub 1}-weighted DCE-MRI data in normal kidneys and to demonstrate that the fitted physiological parameters correlate with published normal values. The model incorporates delay and broadening of the arterial vascular peak as it appears in the capillary bed, two distinct compartments for renal intravascular and extravascular Gd tracer, and uses a small-vessel haematocrit value of 24%. Four physiological parameters can be estimated: regional filtration K{sup trans} (ml min {sup -1} [ml tissue ]{sup -1}), perfusion F (ml min {sup -1} [100 ml tissue ]{sup -1}), blood volume v{sub b} (%) and mean residence time MRT (s). From these are found the filtration fraction (FF; %) and total GFR (ml min {sup -1}). Fifteen healthy volunteers were imaged twice using oblique coronal slices every 2.5 s to determine the reproducibility. Using parenchymal ROIs, group mean values for renal biomarkers all agreed with published values: K{sup trans}: 0.25; F: 219; v{sub b}: 34; MRT: 5.5; FF: 15; GFR: 115. Nominally cortical ROIs consistently underestimated total filtration (by {proportional_to} 50%). Reproducibility was 7-18%. Sensitivity analysis showed that these fitted parameters are most vulnerable to errors in the fixed parameters kidney T{sub 1}, flip angle, haematocrit and relaxivity. These renal biomarkers can potentially measure renal physiology in diagnosis and treatment. circle Dynamic contrast-enhanced magnetic resonance imaging can measure renal function. circle Filtration and perfusion values in healthy volunteers agree with published normal values. circle Precision measured in healthy volunteers is between 7 and 15%. (orig.)

  20. Effects of aortic tortuosity on left ventricular diastolic parameters derived from gated myocardial perfusion single photon emission computed tomography in patients with normal myocardial perfusion.

    Science.gov (United States)

    Kurisu, Satoshi; Nitta, Kazuhiro; Sumimoto, Yoji; Ikenaga, Hiroki; Ishibashi, Ken; Fukuda, Yukihiro; Kihara, Yasuki

    2017-12-05

    Aortic tortuosity is often found on chest radiograph, especially in aged patients. We tested the hypothesis that aortic tortuosity was associated with LV diastolic parameters derived from gated SPECT in patients with normal myocardial perfusion. One-hundred and twenty-two patients with preserved LV ejection fraction and normal myocardial perfusion were enrolled. Descending aortic deviation was defined as the horizontal distance from the left line of the aortic knob to the most prominent left line of the descending aorta. This parameter was measured for the quantitative assessment of aortic tortuosity. Peak filling rate (PFR) and one-third mean filling rate (1/3 MFR) were obtained from redistribution images as LV diastolic parameters. Descending aortic deviation ranged from 0 to 22 mm with a mean distance of 4.5 ± 6.3 mm. Descending aortic deviation was significantly correlated with age (r = 0.38, p < 0.001) and estimated glomerular filtration rate (eGFR) (r = - 0.21, p = 0.02). Multivariate linear regression analysis revealed that eGFR (β = 0.23, p = 0.02) and descending aortic deviation (β = - 0.23, p = 0.01) were significantly associated with PFR, and that only descending aortic deviation (β = - 0.21, p = 0.03) was significantly associated with 1/3 MFR. Our data suggest that aortic tortuosity is associated with LV diastolic parameters derived from gated SPECT in patients with normal myocardial perfusion.

  1. Dr. Buzby’s ToeGrips® Application Results in Minimal Changes in Kinetic Gait Parameters in Normal Dogs

    Directory of Open Access Journals (Sweden)

    James K. Roush

    2017-07-01

    Full Text Available Poor traction on slick surfaces is difficult for dogs with neurologic deficits, osteoarthritis, or recovering from injury or surgery. Many dogs respond inappropriately to slick surfaces by decreasing digital pad-floor contact and extending their toenails. A device marketed to increase paw-floor friction in dogs was evaluated. Fifteen normal dogs underwent kinetic gait analysis before and after application of Dr. Buzby’s ToeGrips®. Ground reaction forces, including vertical peak force (VPF and impulse for each limb, were measured and compared between pre- and post-application values. Stance time was significantly increased in all limbs after toe grip application. Stride velocity was slower in all limbs but significantly slower only in the left forelimb. VPF was significantly deceased in both hindlimbs after toe grip application, but the decrease was within the group SDs. Vertical impulse was significantly increased in both forelimbs and in the right hindlimb. Dr. Buzby’s ToeGrips® result in a slower gait, with slightly decreased VPF in the hindlimbs and increased effort for propulsion kinetic changes were of minor magnitude and unlikely to be clinically relevant.

  2. Some biochemical and hematological changes in female rats under protein malnutrition

    International Nuclear Information System (INIS)

    EL-Sherbiny, E.M.; El-Mahdy, A.A.; Bayoumi, M.M.

    2006-01-01

    The aim of this study was to clarify the effect of low and high dietary protein on some biochemical and hematological parameters in blood of female albino rats. A total number of 75 albino female rats were equally divided into 3 groups, the first group was fed 20% protein diet and served as control and the second and third groups were fed 5% and 65% protein for 5 weeks and served as low and high protein dietary groups, respectively. The results showed high significant decreases in serum growth hormone, ferritin levels and iron concentration in group II and there was significant increase in unsaturated iron binding capacity (UIBC) in group III, compared to control group. Studies of total protein and its fractions revealed high significant decreases in total protein, albumin, alpha-1-globulin, beta-globulin as well as gamma globulin in group II and significant increases in total protein, alpha-1- globulin, beta-globulin and gamma-globulin in group III, compared to normal control group. The hematological investigations in group II revealed significant decreases in hemoglobin value, total leukocyte count, platelets, mean corpuscular hemoglobin concentration (MCHC), erythrocytic count and mean corpuscular volume (MCV). On the other hand, there was significant increase in total leukocyte count in group III if compared to control group

  3. Hematological values for adult eastern Hermann's tortoise (Testudo hermanni boettgeri) in semi-natural conditions.

    Science.gov (United States)

    Bielli, Mattia; Nardini, Giordano; Di Girolamo, Nicola; Savarino, Paolo

    2015-01-01

    Hermann's tortoise (Testudo hermanni) is considered near threatened in the wild but, by contrast, it is one of the most popular pet tortoises in Europe. Scant data is reported in the veterinary literature on hematological values for T. hermanni and, to our knowledge, none focused on the subspecies boettgeri (eastern Hermann's tortoise). Published reports are based on small sample populations, and confusion arises when comparing the sampling sites, the anticoagulants, and the counting methods used. The purpose of the current study was to establish the normal mean values and reference intervals for the main hematological parameters for captive adult T. hermanni boettgeri and to evaluate the reliability of a semiautomated blood analyzer for red blood cell count and hematocrit determination. Blood values were determined in 23 adult tortoises using a Neubauer chamber with Natt and Herrick solution; red blood cells and hematocrit were also measured using a semiautomated blood analyzer. Statistical analysis included descriptive statistics, differences between sexes, and agreement between the counting methods. Reference intervals were calculated with the robust method. Wilcoxon signed rank test with continuity correction was used to investigate differences between sexes, and Bland-Altman analysis was performed to compare manual versus semiautomated values. Red blood cells, hematocrit, and hemoglobin determinations were significantly higher in males than in females. White blood cell counts did not show any sex variability. The agreement of manual versus semiautomated determination was considered acceptable for clinical use. © 2014 The Author(s).

  4. [Oral nutritional supplementation in hematologic patients].

    Science.gov (United States)

    Peñalva, A; San Martín, A; Rosselló, J; Pérez-Portabella, C; Palacios, A; Julià, A; Planas, M

    2009-01-01

    Hematological patients often present anorexia which along with other secondary effects from the chemotherapy and/or radiotherapy treatments compromise their nutritional status. Oral supplementation can aid to fulfill the energy and protein requirements of these patients. Nevertheless, the use of commercial nutritional supplements normally available, is limited by its poor intake. To evaluate the degree of fulfillment of the prescribed supplements and fulfillment of energy requirements, as well as the development of nutritional status in hematological patients hospitalized for treatment with chemotherapy and/or radiotherapy. Prospective, randomized and open study of inpatients at the hematological ward. Patients were randomized sequentially and they were assigned into 3 different nutritional interventions providing: Group 1 (G1), a flavored supplement; Group 2 (G2): a non flavored (neutral) supplement and Group 3 (G3): "kitchen" foods as supplements. Need and amount of nutritional supplements were provided according to the oral intake previously analyzed. Nutritional assessment (at admission and discharge) was based in the Subjective Global Assessment test (SGA), Risk Nutritional Index (RNI) and percentage of lost weight. Both fulfillment of supplement intake and achievement of energetic requirements were analyzed. 125 patients of 51.3 +/- 16.8 years; 45% men and 55% women. 54% lymphoma, 33% leukemia, 8% myeloma and others 4%. Length of stay (LOS): 7.0 +/- 3.6 d. The nutritional assessment done by SGA showed significant negative changes in G2 and G3 (G1: 30% developed malnutrition and 28% improved their nutritional status, p = NS; G2: 50% developed malnutrition against 7% whom improved their nutritional status, p = 0.002; y G3: 37% developed malnutrition against 21% whom improved their nutritional status, p = 0.02). According to RNI, patients evolved negatively from their nutritional state but no significant differences were found within groups (G1, from 81% of

  5. CD44 in hematological neoplasias.

    Science.gov (United States)

    Hertweck, Magdalena Katharina; Erdfelder, Felix; Kreuzer, Karl-Anton

    2011-05-01

    The CD44 protein family spans a large group of transmembrane glycoproteins acquired by alternative splicing and post-translational modifications. The great heterogeneity in molecular structure is reflected in its various important functions: CD44 mediates (1) interaction between cell and extracellular matrix, (2) signal submission, e.g., by acting as co-receptor for membrane-spanning receptor tyrosine kinases or by association with intracellular molecules initiating several signaling pathways, and (3) anchor function connecting to the cytoskeleton via the ezrin-radixin-moesin protein family. The expression pattern of the different CD44 isoforms display strong variations dependent on cell type, state of activation, and differentiation stage. In hematopoietic cells, CD44 mediates interaction of progenitor cells and bone marrow stroma during hematopoiesis, regulates maturation, and activation-induced cell death in T cells, influences neutrophil and macrophage migration as well as cytokine production, and participates in lymphocyte extravasation and migration. CD44 is involved in development and progress of hematological neoplasias by enhancement of apoptotic resistance, invasiveness, as well as regulation of bone marrow homing, and mobilization of leukemia-initiating cells into the peripheral blood. Thereby altered CD44 expression functions as marker for worse prognosis in most hematological malignancies. Additionally, CD44 expression levels can be used to distinguish between different hematological neoplasias and subtypes. Concerning new treatment strategies, CD44 displays promising potential either by direct targeting of CD44 expressed on the malignant cells or reversing an acquired resistance to primary treatment mediated through altered CD44 expression. The former can be achieved by antibody or hyaluronan-based immunotherapy.

  6. B-Cell Hematologic Malignancy Vaccination Registry

    Science.gov (United States)

    2017-12-29

    Monoclonal Gammopathy of Undetermined Significance; Multiple Myeloma; Waldenstrom Macroglobulinemia; Lymphocytosis; Lymphoma, Non-Hodgkin; B-Cell Chronic Lymphocytic Leukemia; Hematological Malignancies

  7. HEMATOLOGICAL INDICES OF RAT ORGANISMS UNDER CONDITIONS OF OXIDATIVE STRESS AND LIPOSOMAL PREPARATION ACTION

    OpenAIRE

    M. Khariv; B. V. Gutyj; V. Butsyak; І. Khariv

    2016-01-01

    The article deals with the results of search of the influence of developed complex liposomal drug on dynamics of hematological parameters of rat organisms under conditions of simulated oxidative stress caused by the use of carbon tetrachloride. Intramuscular injection of 50% tetrachloromethane to rats at a dose of 0.25 ml per 100 g of body weight causes antigenic load on the body and leads to disruption of physiologic level of hematological indices of experimental animal organisms. This indic...

  8. Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats

    OpenAIRE

    Brkić, Dragica; Szakonyne-Pasics, Ilona; Gašić, Slavica; Karan, Vesela; Radivojević, Ljiljana; Nešković, Neško

    2011-01-01

    Hematological effects of the herbicide Avalon (GAL-57), a mixture of bentazon and dicamba, were tested on rats. Avalon was administered by gavage at three and four dose levels (250, 500, 1000 and 1250 mg/kg weight/day) for 28 and 90 days. Hematological parameters, number of erythrocytes, leukocytes and platelets, hemoglobin concentration, hematocrit and erythrocyte indexes (MCV, MCH and MCHC) were monitored. The results showed that the herbicide Avalon caus...

  9. Hematology

    International Nuclear Information System (INIS)

    Ebbe, S.N.; Brecher, G.; Cohen, R.A.

    1981-01-01

    The program at the Donner Clinic in experimental megakaryocytopoiesia is described. Studies are being initiated to analyze blood gases and acid-base balance in patients with erythrocytosis and to correlate these measurements with levels of erythropoietin in blood and urine. The regulation of platelet production in humans and ways in which it may be aberrant in disease states are being investigated. Tracers used in this study include 75 Se-selenomethionine and 35 S-sodium sulfate

  10. Interocular symmetry of retinal nerve fiber layer and optic nerve head parameters measured by Cirrus high-definition optical coherence tomography in a normal pediatric population.

    Science.gov (United States)

    Pawar, Neelam; Maheshwari, Devendra; Ravindran, Meenakshi; Ramakrishnan, Renagappa

    2017-10-01

    To determine interocular differences in the retinal nerve fiber layer (RNFL) and optic nerve head (ONH) parameters in a pediatric population using Cirrus high-definition optical coherence tomography (HD-OCT). Seventy normal Indian children aged 5-17 years presenting to the Pediatric Clinic were included in this observational cross-sectional study. All subjects underwent a comprehensive ophthalmologic examination and an evaluation of the RNFL and ONH by Cirrus HD-OCT. Differences between the right and left eyes were calculated and values were compared by means of a paired t-test. Subjects were also divided into two groups based on age (under or over 10 years of age). Interocular differences in RNFL and ONH parameters together with sex and age variations for these differences were determined. The mean age of studied pediatric population was 11.83 ± 3.3 years (range 5-17). Average RNFL thickness was 94.46 ± 8.7 μm (± SD) (range 77-111). Differences in the average RNFL between right and left eyes were not statistically significant (P = 0.060). Superior quadrant RNFL was thicker in the left eye and temporal quadrant was thicker in the right eye. Among ONH parameters, there were no statistically significant differences in any parameters, except vertical cup-disc (CD) ratio which was significant (P = 0.007). The 2.5%-97.5% limits of asymmetry were 9 μm for average RNFL, 0.14 for average CD ratio, and 0.22 for vertical CD ratio. Mean interocular RNFL thickness differences in superior, superior nasal, and temporal superior quadrants were 10.61 (P sex, while only significant differences with age were observed in 12 clock hour sector analysis, mainly in nasal inferior and inferior quadrant. We report the degree of interocular symmetry of RNFL and ONH parameters measured by Cirrus HD-OCT in a healthy pediatric population. The normal interocular RNFL asymmetry should not exceed 9 μm and vertical CD ratio beyond 0.22 should be considered for further investigations. The

  11. Checkpoint inhibitors in hematological malignancies

    Directory of Open Access Journals (Sweden)

    Chi Young Ok

    2017-05-01

    Full Text Available Abstract Inhibitory molecules such as PD-1, CTLA-4, LAG-3, or TIM-3 play a role to keep a balance in immune function. However, many cancers exploit such molecules to escape immune surveillance. Accumulating data support that their functions are dysregulated in lymphoid neoplasms, including plasma cell myeloma, myelodysplastic syndrome, and acute myeloid leukemia. In lymphoid neoplasms, aberrations in 9p24.1 (PD-L1, PD-L2, and JAK2 locus, latent Epstein-Barr virus infection, PD-L1 3′-untranslated region disruption, and constitutive JAK-STAT pathway are known mechanisms to induce PD-L1 expression in lymphoma cells. Clinical trials demonstrated that PD-1 blockade is an attractive way to restore host’s immune function in hematological malignancies, particularly classical Hodgkin lymphoma. Numerous clinical trials exploring PD-1 blockade as a single therapy or in combination with other immune checkpoint inhibitors in patients with hematologic cancers are under way. Although impressive clinical response is observed with immune checkpoint inhibitors in patients with certain cancers, not all patients respond to immune checkpoint inhibitors. Therefore, to identify best candidates who would have excellent response to checkpoint inhibitors is of utmost importance. Several possible biomarkers are available, but consensus has not been made and pursuit to discover the best biomarker is ongoing.

  12. Checkpoint inhibitors in hematological malignancies.

    Science.gov (United States)

    Ok, Chi Young; Young, Ken H

    2017-05-08

    Inhibitory molecules such as PD-1, CTLA-4, LAG-3, or TIM-3 play a role to keep a balance in immune function. However, many cancers exploit such molecules to escape immune surveillance. Accumulating data support that their functions are dysregulated in lymphoid neoplasms, including plasma cell myeloma, myelodysplastic syndrome, and acute myeloid leukemia. In lymphoid neoplasms, aberrations in 9p24.1 (PD-L1, PD-L2, and JAK2 locus), latent Epstein-Barr virus infection, PD-L1 3'-untranslated region disruption, and constitutive JAK-STAT pathway are known mechanisms to induce PD-L1 expression in lymphoma cells. Clinical trials demonstrated that PD-1 blockade is an attractive way to restore host's immune function in hematological malignancies, particularly classical Hodgkin lymphoma. Numerous clinical trials exploring PD-1 blockade as a single therapy or in combination with other immune checkpoint inhibitors in patients with hematologic cancers are under way. Although impressive clinical response is observed with immune checkpoint inhibitors in patients with certain cancers, not all patients respond to immune checkpoint inhibitors. Therefore, to identify best candidates who would have excellent response to checkpoint inhibitors is of utmost importance. Several possible biomarkers are available, but consensus has not been made and pursuit to discover the best biomarker is ongoing.

  13. Normal value of functional parameters in gated myocardial perfusion SPECT in patients with low risk of coronary artery disease: emory cardiac tool box program

    Energy Technology Data Exchange (ETDEWEB)

    Kang, D. Y.; Kim, M. H.; Kim, Y. D.; Kim, D. K. [Donga University College of Medicine, Busan (Korea, Republic of)

    2002-07-01

    Absolute value of the functional data of gated myocardial perfusion SPECT is necessary to determine that individual patient is normal or not. Tc-99m MIBI gated myocardial perfusion SPECT was performed using emory cardiac tool box program. All patients (M:F=15:36, age 64{+-}10 yrs) showed normal myocardial perfusion. The patients with following characteristics were excluded; previous angina or MI, ECG change with Q wave or ST-T change, diabetes mellitus, hypercholesterolemia, typical chest pain and hypertension. In all patients, myocardial mass is 117{+-}23 g in stress gated SPECT, 106{+-}22 g in stress ungated SPECT and 102{+-}21 g in rest ungated SPECT. EDV is 90{+-}28 ml, ESV 26{+-}20 ml, SV 66{+-}21 ml, EF 73{+-}10 % and TID 1.06{+-}0.14. Myocardial mass in rest ungated SPECT is significantly different between men and women (p=0.025). Myocardial mass is significantly different between stress gated SPECT and stress ungated SPECT (p=0.000), and between stress ungated SPECT and rest ungated SPECT (p=0.003). We provide normal value of functional parameters to determine the abnormality of individual patients in patients with low risk of coronary artery disease.

  14. Cardiac Magnetic Resonance Imaging in Myocarditis Reveals Persistent Disease Activity Despite Normalization of Cardiac Enzymes and Inflammatory Parameters at 3-Month Follow-Up.

    Science.gov (United States)

    Berg, Jan; Kottwitz, Jan; Baltensperger, Nora; Kissel, Christine K; Lovrinovic, Marina; Mehra, Tarun; Scherff, Frank; Schmied, Christian; Templin, Christian; Lüscher, Thomas F; Heidecker, Bettina; Manka, Robert

    2017-11-01

    There is a major unmet need to identify high-risk patients in myocarditis. Although decreasing cardiac and inflammatory markers are commonly interpreted as resolving myocarditis, this assumption has not been confirmed as of today. We sought to evaluate whether routine laboratory parameters at diagnosis predict dynamic of late gadolinium enhancement (LGE) as persistent LGE has been shown to be a risk marker in myocarditis. Myocarditis was diagnosed based on clinical presentation, high-sensitivity troponin T, and cardiac magnetic resonance imaging, after exclusion of obstructive coronary artery disease by angiography. Cardiac magnetic resonance imaging was repeated at 3 months. LGE extent was analyzed with the software GT Volume. Change in LGE >20% was considered significant. Investigated cardiac and inflammatory markers included high-sensitivity troponin T, creatine kinase, myoglobin, N-terminal B-type natriuretic peptide, C-reactive protein, and leukocyte count. Twenty-four patients were enrolled. Absolute levels of cardiac enzymes and inflammatory markers at baseline did not predict change in LGE at 3 months. Cardiac and inflammatory markers had normalized in 21 patients (88%). LGE significantly improved in 16 patients (67%); however, it persisted to a lesser degree in 17 of them (71%) and increased in a small percentage (21%) despite normalization of cardiac enzymes. This is the first study reporting that cardiac enzymes and inflammatory parameters do not sufficiently reflect LGE in myocarditis. Although a majority of patients with normalizing laboratory markers experienced improved LGE, in a small percentage LGE worsened. These data suggest that cardiac magnetic resonance imaging might add value to currently existing diagnostic tools for risk assessment in myocarditis. © 2017 American Heart Association, Inc.

  15. Is liver perfusion CT reproducible? A study on intra- and interobserver agreement of normal hepatic haemodynamic parameters obtained with two different software packages.

    Science.gov (United States)

    Bretas, Elisa Almeida Sathler; Torres, Ulysses S; Torres, Lucas Rios; Bekhor, Daniel; Saito Filho, Celso Fernando; Racy, Douglas Jorge; Faggioni, Lorenzo; D'Ippolito, Giuseppe

    2017-10-01

    To evaluate the agreement between the measurements of perfusion CT parameters in normal livers by using two different software packages. This retrospective study was based on 78 liver perfusion CT examinations acquired for detecting suspected liver metastasis. Patients with any morphological or functional hepatic abnormalities were excluded. The final analysis included 37 patients (59.7 ± 14.9 y). Two readers (1 and 2) independently measured perfusion parameters using different software packages from two major manufacturers (A and B). Arterial perfusion (AP) and portal perfusion (PP) were determined using the dual-input vascular one-compartmental model. Inter-reader agreement for each package and intrareader agreement between both packages were assessed with intraclass correlation coefficients (ICC) and Bland-Altman statistics. Inter-reader agreement was substantial for AP using software A (ICC = 0.82) and B (ICC = 0.85-0.86), fair for PP using software A (ICC = 0.44) and fair to moderate for PP using software B (ICC = 0.56-0.77). Intrareader agreement between software A and B ranged from slight to moderate (ICC = 0.32-0.62) for readers 1 and 2 considering the AP parameters, and from fair to moderate (ICC = 0.40-0.69) for readers 1 and 2 considering the PP parameters. At best there was only moderate agreement between both software packages, resulting in some uncertainty and suboptimal reproducibility. Advances in knowledge: Software-dependent factors may contribute to variance in perfusion measurements, demanding further technical improvements. AP measurements seem to be the most reproducible parameter to be adopted when evaluating liver perfusion CT.

  16. High grade intraepithelial neoplasia of prostate is associated with values of prostate specific antigen related parameters intermediate between prostate cancer and normal levels.

    Science.gov (United States)

    Obralic, Nermina; Kulovac, Benjamin

    2011-11-01

    High grade prostatic intraepithelial neoplasia (HGPIN) is widely regarded as the precancerous. The aim of this study was to determine PSA related parameters in patients with initial PSA values 2-10 ng/mL and diagnosis of HGPIN without finding carcinoma at the time of their first needle biopsy. Study groups consisted of 100 men who were diagnosed HGPIN, 84 with cancer and 183 with benign hyperplasia on first biopsy of prostate. Total PSA and free PSA were measured and ratio free/total PSA and PSA density calculated. Mean values of these parameters were compared, and receiver operating characteristic curves were used for comparison of PSA related parameters to discriminate groups of patients.Total PSA, free PSA level and PSA density in patients with HGPIN (6.388 ng/mL) did not differ significantly compared to prostate carcinoma (6.976 ng/mL) or benign prostatic hyperplasia (6.07 ng/mL) patients. Patients with HGPIN had significantly higher ratio free/total PSA than those with prostate carcinoma (0.168 vs 0.133), but significantly lower than patients with benign prostatic hyperplasia (0.168 vs 0.185). Ratio of free/total PSA significantly discriminate HGPIN from prostate carcinoma with sensitivity 84.52 and specify 45.00 at cut-off point of ≤ 0.18. Values of PSA, free PSA and ratio free/total PSA in cases of HGPIN appear to be intermediate between prostate cancer and normal levels. Ratio of free/total PSA may help in decision to repeat biopsies in the presence of HGPIN on biopsy, without concomitant prostate cancer, in patients suitable for curative treatment, with normal digito-rectal examination and trans-rectal sonography.

  17. High grade intraepithelial neoplasia of prostate is associated with values of prostate specific antigen related parameters intermediate between prostate cancer and normal levels

    Directory of Open Access Journals (Sweden)

    Nermina Obralic

    2011-11-01

    Full Text Available High grade prostatic intraepithelial neoplasia (HGPIN is widely regarded as the precancerous. The aim of this study was to determine PSA related parameters in patients with initial PSA values 2-10 ng/mL and diagnosis of HGPIN without finding carcinoma at the time of their first needle biopsy. Study groups consisted of 100 men who were diagnosed HGPIN, 84 with cancer and 183 with benign hyperplasia on first biopsy of prostate. Total PSA and free PSA were measured and ratio free/total PSA and PSA density calculated. Mean values of these parameters were compared, and receiver operating characteristic curves were used for comparison of PSA related parameters to discriminate groups of patients. Total PSA, free PSA level and PSA density in patients with HGPIN (6.388 ng/mL did not differ significantly compared to prostate carcinoma (6.976 ng/mL or benign prostatic hyperplasia (6.07 ng/mL patients. Patients with HGPIN had significantly higher ratio free/total PSA than those with prostate carcinoma (0.168 vs 0.133, but significantly lower than patients with benign prostatic hyperplasia (0.168 vs 0.185. Ratio of free/total PSA significantly discriminate HGPIN from prostate carcinoma with sensitivity 84.52 and specify 45.00 at cut-off point of ≤ 0.18. Values of PSA, free PSA and ratio free/total PSA in cases of HGPIN appear to be intermediate between prostate cancer and normal levels. Ratio of free/total PSA may help in decision to repeat biopsies in the presence of HGPIN on biopsy, without concomitant prostate cancer, in patients suitable for curative treatment, with normal digito-rectal examination and trans-rectal sonography.

  18. 42 CFR 493.851 - Standard; Hematology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard; Hematology. 493.851 Section 493.851 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... These Tests § 493.851 Standard; Hematology. (a) Failure to attain a score of at least 80 percent of...

  19. 42 CFR 493.1269 - Standard: Hematology.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard: Hematology. 493.1269 Section 493.1269 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Systems § 493.1269 Standard: Hematology. (a) For manual cell counts performed using a hemocytometer— (1...

  20. 15th Congress of European Hematology Association

    NARCIS (Netherlands)

    Chomienne, Christine; Guenova, Margarita; Hagenbeek, Antony; Lacombe, Catherine; McCann, Shaun; Foa, Robin

    2010-01-01

    Each year the annual congress of the European Hematology Association gathers clinicians, biologists and scientists dedicated to all fields of hematology. This year's Congress was held in Barcelona, Spain, and presented an appealing program with experts presenting state-of-the-art sessions to more

  1. Hematology and biochemistry reference intervals for Ontario commercial nursing pigs close to the time of weaning.

    Science.gov (United States)

    Perri, Amanda M; O'Sullivan, Terri L; Harding, John C S; Wood, R Darren; Friendship, Robert M

    2017-04-01

    The evaluation of pig hematology and biochemistry parameters is rarely done largely due to the costs associated with laboratory testing and labor, and the limited availability of reference intervals needed for interpretation. Within-herd and between-herd biological variation of these values also make it difficult to establish reference intervals. Regardless, baseline reference intervals are important to aid veterinarians in the interpretation of blood parameters for the diagnosis and treatment of diseased swine. The objective of this research was to provide reference intervals for hematology and biochemistry parameters of 3-week-old commercial nursing piglets in Ontario. A total of 1032 pigs lacking clinical signs of disease from 20 swine farms were sampled for hematology and iron panel evaluation, with biochemistry analysis performed on a subset of 189 randomly selected pigs. The 95% reference interval, mean, median, range, and 90% confidence intervals were calculated for each parameter.

  2. [A comparative study on the absorption kinetics parameters of rhubarb free anthraquinones between normal dogs and dogs with severe acute pancreatitis].

    Science.gov (United States)

    Yang, Yong-Mao; Wang, Ping; Zhang, Yan

    2012-04-01

    To study the effects of severe acute pancreatitis (SAP) on absorption kinetic parameters of rhubarb free anthraquinones. Eleven healthy Beagle dogs were randomly divided into the normal group (n = 6) and the SAP group (n = 5). The SAP animal model was prepared by surgery through portal vein blood channel building to collect blood from normal dogs and dogs with SAP. The free anthraquinones (20 mg/kg) was given by gastrogavage. The concentrations of five anthraquinones (aloe emodin, rhein, emodin, chrysophanol, and physcion) in the blood plasma of the portal vein and the femoral artery were determined using high performance liquid chromatography (HPLC). The kinetic parameters were calculated using MATLAB2007B Software. The half life (t(1/2Ka)), the absorption peak time (Tmax), the peak concentration (Cmax), the area under the curve [AUC(0-infinity)], and the mean residence time (MRT) were calculated using the statistical moment method. The transport velocity of corresponding medicines from the gastrointestinal tract to the blood (ka) was calculated. There was no difference in the chemical composition absorption type of the portal vein and the femoral artery between the two groups. Aloe emodin could be detected in the portal vein of each animal at each time point, and they were in the quantitative range. Rhein could be detected in the portal vein of each animal at each time point, and they were lower than the quantitative limit at few time points. Emodin and chrysophanol could be detected in the portal vein of partial animals at each time point, and most of them were higher than the quantitative limit. Physcion could be detected only in the portal vein of less animals at few time points. Rhein could be detected in the femoral artery at most time points, but the rhein plasma concentration at most time points were lower than the quantitative limit. Lower concentration of aloe emodin, emodin, and chrysophanol could be detected in the femoral artery at only few time

  3. Performance evaluation of Samsung LABGEO(HC10) Hematology Analyzer.

    Science.gov (United States)

    Park, Il Joong; Ahn, Sunhyun; Kim, Young In; Kang, Seon Joo; Cho, Sung Ran

    2014-08-01

    The Samsung LABGEO(HC10) Hematology Analyzer (LABGEO(HC10)) is a recently developed automated hematology analyzer that uses impedance technologies. The analyzer provides 18 parameters including 3-part differential at a maximum rate of 80 samples per hour. To evaluate the performance of the LABGEO(HC10). We evaluated precision, linearity, carryover, and relationship for complete blood cell count parameters between the LABGEO(HC10) and the LH780 (Beckman Coulter Inc) in a university hospital in Korea according to the Clinical and Laboratory Standards Institute guidelines. Sample stability and differences due to the anticoagulant used (K₂EDTA versus K₃EDTA) were also evaluated. The LABGEO(HC10) showed linearity over a wide range and minimal carryover ( 0.92) except for mean corpuscular hemoglobin concentration. The bias estimated was acceptable for all parameters investigated except for monocyte count. Most parameters were stable until 24 hours both at room temperature and at 4°C. The difference by anticoagulant type was statistically insignificant for all parameters except for a few red cell parameters. The accurate results achievable and simplicity of operation make the unit recommendable for small to medium-sized laboratories.

  4. Skylab experiment results: Hematology studies

    Science.gov (United States)

    Kimzey, S. L.; Ritzmann, S. E.; Mengel, C. E.; Fischer, C. L.

    1975-01-01

    Studies were conducted to evaluate specific aspects of man's immunologic and hematologic systems that might be altered by or respond to the space flight environment. Biochemical functions investigated included cytogenetic damage to blood cells, immune resistance to disease, regulation of plasma and red cell volumes, metabolic processes of the red blood cell, and physicochemical aspects of red blood cell function. Measurements of hematocrit value showed significant fluctuations postflight, reflecting observed changes in red cell mass and plasma volume. The capacity of lymphocytes to respond to an in vitro mitogenic challenge was repressed postflight, and appeared to be related to mission duration. Most other deviations from earth function in these systems were minor or transient.

  5. Radioprotective potential of Emblica officinalis fruit extract against hematological alterations induced by gamma radiation

    International Nuclear Information System (INIS)

    Singh, Inder; Soyal, Dhanraj; Goyal, P.K.

    2012-01-01

    In the era of expending nuclear energy program all over the world, the role of radiation biology has acquired greater relevance and significance in addressing the health issues in view of constant human exposure to various types of radiations. Radioprotective drugs hold immense promise for saving precious human lives in from irradiation in various situations. Currently available synthetic radiomudulators is fraught with their inherent toxicity at the optimum dose and hence the need to discover and develop new more effective less toxic radiomudulatory drugs from natural sources. In the present study, the protective effect of Emblica officinalis fruit extract (EOFE) has been assessed by estimating hematological constituents against irradiation. For this purpose, Swiss albino mice were divided into four groups. Group I was administered with double distilled water (DDW) volume equal to EOFE (100 mg./kg. body wt./animal/clay) by oral gavages to serve as normal. Group II was administered orally EOFE for 7 days once daily at a does of 100 mg./kg.b. wt./animal/day, Group Ill animals were exposed to 2.5 Gy gamma radiations to serve as irradiated control. Group IV mice were treated with EOFE, orally for consecutive days (as in Group II) and were exposed to 2.5 Gy gamma rays half an hr. after the last administration of EOFE on day 7th. The above animals were necropsied on 12 hr, 24 hr, 3 days, 5 days, 10 days, 20 days and 30 days post treatment intervals, and their blood was collected for estimation of blood constituents. A significant decline in RBC, hemoglobin (Hb) and hematocrit (Hct) contents from normal was observed in irradiated control animals (Group III). All these parameters were found to be significantly higher in EOFE pretreated irradiated animals (Group IV). From these results, it is concluded that Emblica officinalis fruit extract has the ability to protect the individuals from radiation-induced hematological injuries. (author)

  6. Normal tissue complication probability model parameter estimation for xerostomia in head and neck cancer patients based on scintigraphy and quality of life assessments

    Directory of Open Access Journals (Sweden)

    Lee Tsair-Fwu

    2012-12-01

    Full Text Available Abstract Background With advances in modern radiotherapy (RT, many patients with head and neck (HN cancer can be effectively cured. However, xerostomia is a common complication in patients after RT for HN cancer. The purpose of this study was to use the Lyman–Kutcher–Burman (LKB model to derive parameters for the normal tissue complication probability (NTCP for xerostomia based on scintigraphy assessments and quality of life (QoL questionnaires. We performed validation tests of the Quantitative Analysis of Normal Tissue Effects in the Clinic (QUANTEC guidelines against prospectively collected QoL and salivary scintigraphic data. Methods Thirty-one patients with HN cancer were enrolled. Salivary excretion factors (SEFs measured by scintigraphy and QoL data from self-reported questionnaires were used for NTCP modeling to describe the incidence of grade 3+ xerostomia. The NTCP parameters estimated from the QoL and SEF datasets were compared. Model performance was assessed using Pearson’s chi-squared test, Nagelkerke’s R2, the area under the receiver operating characteristic curve, and the Hosmer–Lemeshow test. The negative predictive value (NPV was checked for the rate of correctly predicting the lack of incidence. Pearson’s chi-squared test was used to test the goodness of fit and association. Results Using the LKB NTCP model and assuming n=1, the dose for uniform irradiation of the whole or partial volume of the parotid gland that results in 50% probability of a complication (TD50 and the slope of the dose–response curve (m were determined from the QoL and SEF datasets, respectively. The NTCP-fitted parameters for local disease were TD50=43.6 Gy and m=0.18 with the SEF data, and TD50=44.1 Gy and m=0.11 with the QoL data. The rate of grade 3+ xerostomia for treatment plans meeting the QUANTEC guidelines was specifically predicted, with a NPV of 100%, using either the QoL or SEF dataset. Conclusions Our study shows the agreement

  7. Normal tissue complication probability model parameter estimation for xerostomia in head and neck cancer patients based on scintigraphy and quality of life assessments

    International Nuclear Information System (INIS)

    Lee, Tsair-Fwu; Chao, Pei-Ju; Wang, Hung-Yu; Hsu, Hsuan-Chih; Chang, PaoShu; Chen, Wen-Cheng

    2012-01-01

    With advances in modern radiotherapy (RT), many patients with head and neck (HN) cancer can be effectively cured. However, xerostomia is a common complication in patients after RT for HN cancer. The purpose of this study was to use the Lyman–Kutcher–Burman (LKB) model to derive parameters for the normal tissue complication probability (NTCP) for xerostomia based on scintigraphy assessments and quality of life (QoL) questionnaires. We performed validation tests of the Quantitative Analysis of Normal Tissue Effects in the Clinic (QUANTEC) guidelines against prospectively collected QoL and salivary scintigraphic data. Thirty-one patients with HN cancer were enrolled. Salivary excretion factors (SEFs) measured by scintigraphy and QoL data from self-reported questionnaires were used for NTCP modeling to describe the incidence of grade 3 + xerostomia. The NTCP parameters estimated from the QoL and SEF datasets were compared. Model performance was assessed using Pearson’s chi-squared test, Nagelkerke’s R 2 , the area under the receiver operating characteristic curve, and the Hosmer–Lemeshow test. The negative predictive value (NPV) was checked for the rate of correctly predicting the lack of incidence. Pearson’s chi-squared test was used to test the goodness of fit and association. Using the LKB NTCP model and assuming n=1, the dose for uniform irradiation of the whole or partial volume of the parotid gland that results in 50% probability of a complication (TD 50 ) and the slope of the dose–response curve (m) were determined from the QoL and SEF datasets, respectively. The NTCP-fitted parameters for local disease were TD 50 =43.6 Gy and m=0.18 with the SEF data, and TD 50 =44.1 Gy and m=0.11 with the QoL data. The rate of grade 3 + xerostomia for treatment plans meeting the QUANTEC guidelines was specifically predicted, with a NPV of 100%, using either the QoL or SEF dataset. Our study shows the agreement between the NTCP parameter modeling based on SEF and

  8. Normal tissue complication probability model parameter estimation for xerostomia in head and neck cancer patients based on scintigraphy and quality of life assessments

    Science.gov (United States)

    2012-01-01

    Background With advances in modern radiotherapy (RT), many patients with head and neck (HN) cancer can be effectively cured. However, xerostomia is a common complication in patients after RT for HN cancer. The purpose of this study was to use the Lyman–Kutcher–Burman (LKB) model to derive parameters for the normal tissue complication probability (NTCP) for xerostomia based on scintigraphy assessments and quality of life (QoL) questionnaires. We performed validation tests of the Quantitative Analysis of Normal Tissue Effects in the Clinic (QUANTEC) guidelines against prospectively collected QoL and salivary scintigraphic data. Methods Thirty-one patients with HN cancer were enrolled. Salivary excretion factors (SEFs) measured by scintigraphy and QoL data from self-reported questionnaires were used for NTCP modeling to describe the incidence of grade 3+ xerostomia. The NTCP parameters estimated from the QoL and SEF datasets were compared. Model performance was assessed using Pearson’s chi-squared test, Nagelkerke’s R2, the area under the receiver operating characteristic curve, and the Hosmer–Lemeshow test. The negative predictive value (NPV) was checked for the rate of correctly predicting the lack of incidence. Pearson’s chi-squared test was used to test the goodness of fit and association. Results Using the LKB NTCP model and assuming n=1, the dose for uniform irradiation of the whole or partial volume of the parotid gland that results in 50% probability of a complication (TD50) and the slope of the dose–response curve (m) were determined from the QoL and SEF datasets, respectively. The NTCP-fitted parameters for local disease were TD50=43.6 Gy and m=0.18 with the SEF data, and TD50=44.1 Gy and m=0.11 with the QoL data. The rate of grade 3+ xerostomia for treatment plans meeting the QUANTEC guidelines was specifically predicted, with a NPV of 100%, using either the QoL or SEF dataset. Conclusions Our study shows the agreement between the NTCP

  9. Energy Cost and Gait Efficiency of Below-Knee Amputee and Normal Subject with Similar Physical Parameters & Quality of Life: A Comparative Case Study

    Directory of Open Access Journals (Sweden)

    Durbadal Biswas

    2010-10-01

    Full Text Available The study focused on the comparative analysis of energy cost and gait efficiency between a below knee (BK amputee and a reference subject (without amputation. It also attempted to indicate the specific feature responsible for a controlled gait with optimum energy cost for BK amputees. Selection criteria of the subjects were similar physical parameters and quality of life studied with WHOQOL-100 quality of life assessment. A Cosmed® k4 b2 Respiratory Analyzer system was used for the measurement of Oxygen Uptake (VO2, Energy Expenditure per minute (EE and Heart Rate (HR. Gait efficiency (p < 0.0002 was found higher for BK amputee than normal subject. The therapeutic activities and mainly walking rhythm contributed to improve the mobility & balance. This ensures the optimum time & co-ordination of movements and hence improves the gait efficiency for the BK amputee. Comparison with control group was performed to validate the data.

  10. Quantitative perfusion parameters in a cohort of patients with no known ischemic heart disease and normal myocardial perfusion imaging studied by 82Rb-PET

    DEFF Research Database (Denmark)

    Hoff, Camilla; Balche, Abdallah; Majgaard, J

    -month period were screened for eligibility. One hundred and eighty patients with no prior history of ischemic heart disease, a normal MPI scan defined by visual interpretation and no coronary events for a follow-up period of 6 months were included as normal. Quantitative parameters were calculated using.......86 mL/g/min. Global coronary flow reserve (CFR) was 2.81 (±SD 0.71). EF at rest was 65.3% (±SD 10) and during stress 69% (±SD 12.3), yielding an EF reserve of 4.5%. TPD at rest and stress was 6% (±SD 4). Conclusion Based on a representative population of patients in which coronary artery disease......Purpose 82Rb perfusion PET allows for visual as well as quantitative interpretation of myocardial function. Whereas visual interpretation relies on intra-individual redistribution of the tracer between rest and stress studies, quantitative interpretation of absolute flow values requires robust...

  11. Circuit resistance training in women with normal weight obesity syndrome: body composition, cardiometabolic and echocardiographic parameters, and cardiovascular and skeletal muscle fitness.

    Science.gov (United States)

    Ferreira, Fabiano C; Bertucci, Danilo R; Barbosa, Marina R; Nunes, João E; Botero, João P; Rodrigues, Maria F; Shiguemoto, Gilberto E; Santoro, Valdir; Verzola, Ana C; Nonaka, Rodrigo O; Verzola, Roberto M; Baldissera, Vilmar; Perez, Sérgio E

    2017-01-01

    Normal weight obesity (NWO) syndrome has been characterized in subjects with normal Body Mass Index (BMI) and high body fat mass percentage (BF%>30 for women) being a risk factor for cardiometabolic dysregulation and cardiovascular mortality. This study evaluated whether circuit resistance training (CRT) improves body composition, heart size and function, cardiometabolic parameters, and cardiorespiratory, cardiovascular and skeletal muscle fitness in women with NWO. Data are means (95% Confidence Interval). Twenty-three women participated: 10 NWO-CRT (baseline: BMI=22.4 [21.4-23.3] kg/m2; BF%=44.5 [41.0-48.0]%) performed CRT; and 13 untrained NWO-control (baseline: BMI=21.7 [20.8-22.7] kg/m2; BF%=37.8 [34.6-41.1]%). At baseline and after 10 weeks were performed/measured dual-energy X-ray absorptiometry, echocardiography, blood tests, arterial pressure, exercise testing, and total-overload-by-training-session (TOL). At baseline, the NWO-CRT exhibited larger BF (27.28 [23.9-30.6] kg) than NWO-control (22.41 [19.5-25.3] kg) (P=0.0227). After training, NWO-CRT: reduced 8 kg of BF (P=0.000002); became BF% lower than NWO-control (33.1 [30.1-36.0] body surface area ratio (84.29 [78.98-89.6] to 90.29 [81.45-99.12] g/m2, P=0.0215). CRT reduced BF% and generated cardiometabolic, cardiac, skeletal muscle and cardiovascular benefits, being a useful strategy to combat the normal weight obesity syndrome in women.

  12. Hematologic and biochemical changes during canine agility competitions.

    Science.gov (United States)

    Rovira, S; Muñoz, A; Benito, M

    2007-03-01

    Normal response to different types of exercise needs to be established before performance, training level, or exercise intolerance can be assessed. The aim of this research was to describe the hematologic and biochemical changes that dogs undergo during an agility competition. Fifteen dogs were subjected to an agility test that covered a distance of 360-400 m with 40 obstacles. Basal venous blood samples were drawn 1 week before the competition, immediately after exercise, and at 5, 15, and 30 minutes of recuperation. A CBC, plasma biochemical profile, and lactate concentration were determined by standard methods using automated analyzers. Serum cortisol concentration was measured by competitive immune-enzyme analysis; insulin was measured by sandwich ELISA. Hematologic response to the agility test consisted of significant increases in RBC count, hemoglobin concentration, and HCT, with no change in total protein concentration. Serum triglyceride concentrations were increased postexercise, with highest values observed at 30 minutes of recuperation. Postexercise lactate concentrations exceeded the anaerobic threshold of 4 mmol/L. Serum lactate dehydrogenase activity was significantly higher after 30 minutes of recuperation. Significant differences were not observed in other analytes. Our results indicate that agility competitions induce mild to moderate changes in hematologic and biochemical results consistent with splenic contraction, increased lipolysis, and utilization of anaerobic pathways involved in energy resynthesis in muscle.

  13. Hematologic disorders in trauma patients during parenteral alimentation with lipids.

    Science.gov (United States)

    Faintuch, J; Machado, F K; Freire, A N; Reis, J R; Machado, M; Pinto, L P; Ramos, S M; Loebens, M; Jovchelevich, V; Pinotti, H W

    1996-01-01

    Total parenteral nutrition with lipids is a well-accepted modality of metabolic support in seriously ill trauma patients. Intolerance to lipid administration is unusual when dosage limits are not exceeded, and few hematologic disturbances have been recorded with modern fat emulsions. In the course of intravenous alimentation of six adults admitted for traumatic lesions, eosinophilia with or without leukocytopenia was noticed after periods of four days to five weeks. Principal clinical events and hematologic derangements were documented in this population. Sepsis was not always present in the patients by the time of the complication, and in those that did require antibiotics and other drugs, the prescription remained unchanged along the episode. Discontinuation of the nutritional regimen with lipids was followed by normalization of the hematologic profile, suggesting that an acute or sub-acute allergic reaction was responsible. The appearance of skin rash in two occasions reinforces this hypothesis, and the possibility of hemophagocytosis merits consideration in two of the cases who displayed reversible acute leukocytopenia. It is concluded that blood cell aberrations are possible during intravenous feeding with lipids in trauma subjects, but tend to respond to suppression of the lipid-containing nutritional prescription.

  14. Hematological abnormalities and 22q11.2 deletion syndrome

    Directory of Open Access Journals (Sweden)

    Rafael Fabiano Machado Rosa

    2011-01-01

    Full Text Available The 22q11.2 deletion syndrome (22q11DS is a common genetic disease characterized by broad phenotypic variability. Despite the small number of studies describing hematological alterations in individuals with 22q11DS, it appears that these abnormalities are more frequent than previously imagined. Thus, the objective of our study was to report on a patient with 22q11DS presenting thrombocytopenia and large platelets and to review the literature. The patient, a 13-year-old boy, was originally evaluated due to craniofacial dysmorphia and speech delay. He also had a history of behavioral changes, neuropsychomotor delay and recurrent otitis/sinusitis. The identification of a 22q11.2 microdeletion by fluorescent in situ hybridization diagnosed the syndrome. Despite his hematological alterations, he only had a history of epistaxis and bruising of the upper and lower limbs. Assessments of the prothrombin time, thrombin time, partial thromboplastin time, bleeding time, fibrinogen levels and platelet aggregation (including the ristocetin induced platelet aggregation test were all normal. Hematological alterations observed in 22q11DS are directly related to the genetic disorder itself (especially in respect to deletion of the GPIb gene and secondary to some clinical findings, such as immunodeficiency. Macrothrombocytopenia is increasingly being considered a feature of the broad spectrum of 22q11DS and may potentially be a clinical marker for the syndrome.

  15. Baseline hematology and serum biochemistry results for Indian leopards (Panthera pardus fusca

    Directory of Open Access Journals (Sweden)

    Arun Attur Shanmugam

    2017-07-01

    Full Text Available Aim: The aim of the study was to establish the baseline hematology and serum biochemistry values for Indian leopards (Panthera pardus fusca, and to assess the possible variations in these parameters based on age and gender. Materials and Methods: Hemato-biochemical test reports from a total of 83 healthy leopards, carried out as part of routine health evaluation in Bannerghatta Biological Park and Manikdoh Leopard Rescue Center, were used to establish baseline hematology and serum biochemistry parameters for the subspecies. The hematological parameters considered for the analysis included hemoglobin (Hb, packed cell volume, total erythrocyte count (TEC, total leukocyte count (TLC, mean corpuscular volume (MCV, mean corpuscular Hb (MCH, and MCH concentration. The serum biochemistry parameters considered included total protein (TP, albumin, globulin, aspartate aminotransferase, alanine aminotransferase (ALT, blood urea nitrogen, creatinine, triglycerides, calcium, and phosphorus. Results: Even though few differences were observed in hematologic and biochemistry values between male and female Indian leopards, the differences were statistically not significant. Effects of age, however, were evident in relation to many hematologic and biochemical parameters. Sub-adults had significantly greater values for Hb, TEC, and TLC compared to adults and geriatric group, whereas they had significantly lower MCV and MCH compared to adults and geriatric group. Among, serum biochemistry parameters the sub-adult age group was observed to have significantly lower values for TP and ALT than adult and geriatric leopards. Conclusion: The study provides a comprehensive analysis of hematologic and biochemical parameters for Indian leopards. Baselines established here will permit better captive management of the subspecies, serve as a guide to assess the health and physiological status of the free ranging leopards, and may contribute valuable information for making

  16. Repeatability and Reproducibility of Retinal Nerve Fiber Layer Parameters Measured by Scanning Laser Polarimetry with Enhanced Corneal Compensation in Normal and Glaucomatous Eyes

    Directory of Open Access Journals (Sweden)

    Mirian Ara

    2015-01-01

    Full Text Available Objective. To assess the intrasession repeatability and intersession reproducibility of peripapillary retinal nerve fiber layer (RNFL thickness parameters measured by scanning laser polarimetry (SLP with enhanced corneal compensation (ECC in healthy and glaucomatous eyes. Methods. One randomly selected eye of 82 healthy individuals and 60 glaucoma subjects was evaluated. Three scans were acquired during the first visit to evaluate intravisit repeatability. A different operator obtained two additional scans within 2 months after the first session to determine intervisit reproducibility. The intraclass correlation coefficient (ICC, coefficient of variation (COV, and test-retest variability (TRT were calculated for all SLP parameters in both groups. Results. ICCs ranged from 0.920 to 0.982 for intravisit measurements and from 0.910 to 0.978 for intervisit measurements. The temporal-superior-nasal-inferior-temporal (TSNIT average was the highest (0.967 and 0.946 in normal eyes, while nerve fiber indicator (NFI; 0.982 and inferior average (0.978 yielded the best ICC in glaucomatous eyes for intravisit and intervisit measurements, respectively. All COVs were under 10% in both groups, except NFI. TSNIT average had the lowest COV (2.43% in either type of measurement. Intervisit TRT ranged from 6.48 to 12.84. Conclusions. The reproducibility of peripapillary RNFL measurements obtained with SLP-ECC was excellent, indicating that SLP-ECC is sufficiently accurate for monitoring glaucoma progression.

  17. Repeatability and Reproducibility of Retinal Nerve Fiber Layer Parameters Measured by Scanning Laser Polarimetry with Enhanced Corneal Compensation in Normal and Glaucomatous Eyes.

    Science.gov (United States)

    Ara, Mirian; Ferreras, Antonio; Pajarin, Ana B; Calvo, Pilar; Figus, Michele; Frezzotti, Paolo

    2015-01-01

    To assess the intrasession repeatability and intersession reproducibility of peripapillary retinal nerve fiber layer (RNFL) thickness parameters measured by scanning laser polarimetry (SLP) with enhanced corneal compensation (ECC) in healthy and glaucomatous eyes. One randomly selected eye of 82 healthy individuals and 60 glaucoma subjects was evaluated. Three scans were acquired during the first visit to evaluate intravisit repeatability. A different operator obtained two additional scans within 2 months after the first session to determine intervisit reproducibility. The intraclass correlation coefficient (ICC), coefficient of variation (COV), and test-retest variability (TRT) were calculated for all SLP parameters in both groups. ICCs ranged from 0.920 to 0.982 for intravisit measurements and from 0.910 to 0.978 for intervisit measurements. The temporal-superior-nasal-inferior-temporal (TSNIT) average was the highest (0.967 and 0.946) in normal eyes, while nerve fiber indicator (NFI; 0.982) and inferior average (0.978) yielded the best ICC in glaucomatous eyes for intravisit and intervisit measurements, respectively. All COVs were under 10% in both groups, except NFI. TSNIT average had the lowest COV (2.43%) in either type of measurement. Intervisit TRT ranged from 6.48 to 12.84. The reproducibility of peripapillary RNFL measurements obtained with SLP-ECC was excellent, indicating that SLP-ECC is sufficiently accurate for monitoring glaucoma progression.

  18. Results from transcranial Doppler examination on children and adolescents with sickle cell disease and correlation between the time-averaged maximum mean velocity and hematological characteristics: a cross-sectional analytical study

    Directory of Open Access Journals (Sweden)

    Mary Hokazono

    Full Text Available CONTEXT AND OBJECTIVE: Transcranial Doppler (TCD detects stroke risk among children with sickle cell anemia (SCA. Our aim was to evaluate TCD findings in patients with different sickle cell disease (SCD genotypes and correlate the time-averaged maximum mean (TAMM velocity with hematological characteristics. DESIGN AND SETTING: Cross-sectional analytical study in the Pediatric Hematology sector, Universidade Federal de São Paulo. METHODS: 85 SCD patients of both sexes, aged 2-18 years, were evaluated, divided into: group I (62 patients with SCA/Sß0 thalassemia; and group II (23 patients with SC hemoglobinopathy/Sß+ thalassemia. TCD was performed and reviewed by a single investigator using Doppler ultrasonography with a 2 MHz transducer, in accordance with the Stroke Prevention Trial in Sickle Cell Anemia (STOP protocol. The hematological parameters evaluated were: hematocrit, hemoglobin, reticulocytes, leukocytes, platelets and fetal hemoglobin. Univariate analysis was performed and Pearson's coefficient was calculated for hematological parameters and TAMM velocities (P < 0.05. RESULTS: TAMM velocities were 137 ± 28 and 103 ± 19 cm/s in groups I and II, respectively, and correlated negatively with hematocrit and hemoglobin in group I. There was one abnormal result (1.6% and five conditional results (8.1% in group I. All results were normal in group II. Middle cerebral arteries were the only vessels affected. CONCLUSION: There was a low prevalence of abnormal Doppler results in patients with sickle-cell disease. Time-average maximum mean velocity was significantly different between the genotypes and correlated with hematological characteristics.

  19. Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken

    Directory of Open Access Journals (Sweden)

    Deepesh Bharat Mishra

    2016-02-01

    Full Text Available Aim: The present study was conducted to see the effect of feeding different levels of Azolla meal on blood biochemicals, hematology and immunocompetence traits of Chabro chicken. Materials and Methods: The study was conducted on 160 Chabro chicks, which were randomly divided into four treatment groups each with four replicates of 10 birds. The first treatment (T1 served as a control in which basal diets was offered without Azolla supplementation while in T2, T3, and T4 groups, basal diet was replaced with Azolla meal at 5%, 7.5%, and 10% levels, respectively. A feeding trial was conducted upto 8 weeks. At the last week of trial, blood samples were collected randomly from one bird of each replicate and plasma was separated to estimate certain biochemical parameters, some blood metabolites, minerals and enzymes like alanine aminotransferase and aspartate aminotransferase (AST. Hematological parameters such as hemoglobin, packed cell volume, total leukocytes count and differential leukocytes count were estimated in fresh blood just after collection. The humoral immune response was measured against sheep red blood cells,and cell-mediated immune response was measured against phyto hemagglutinin lectin from Phaseolus vulgaris (PHA-P. Results: The study showed that hematological profile of the Chabro bird was not affected by any treatment except heterophil and lymphocyte which was found higher in T2 and T3 groups and eosinophil was found higher in a T3 group than control. Blood glucose, creatinine, cholesterol, total protein, albumin, uric acid, and triglycerides were found similar in all the groups and within the normal values for broiler chicken. Liver enzymes and macro mineral content in blood were found similar in all the treatment groups and within normal physiological range. Although AST was found higher in 10% replacement group than control, the value was within normal range for broiler chicken. Although antibody titer was found similar in all

  20. ASVCP guidelines: Allowable total error hematology.

    Science.gov (United States)

    Nabity, Mary B; Harr, Kendal E; Camus, Melinda S; Flatland, Bente; Vap, Linda M

    2018-02-11

    The purpose of this document is to provide total allowable error (TE a ) recommendations for commonly analyzed hematology measurands for veterinary personnel. These guidelines define relevant terminology and highlight considerations specific to hematology measurands. They also provide reasons and guidelines for using TE a in instrument performance evaluation, including recommendations for when the total observed error exceeds the recommended TE a . Biological variation-based quality specifications are briefly discussed. The appendix describes the derivation of the hematology TE a recommendations and provides resources for external quality assurance/proficiency testing programs and a worksheet for implementation of the guidelines. © 2018 American Society for Veterinary Clinical Pathology.

  1. 1976 Hanford Americium exposure incident: hematologic effects

    International Nuclear Information System (INIS)

    Ragan, H.A.; Mahaffey, J.A.; Breitenstein, B.D.

    1982-05-01

    Hematologic evaluation of an individual with an initial systemic body burden of approx. 200 μCi 241 Am revealed a significant (P < 0.01) reduction of total leukocytes, neutrophils, and lymphocytes. This effect on total leukocytes and neutrophils was evident approx. 30 days after exposure, appeared to stabilize at about 3 months after exposure, and remained at this lower level thorugh a 52-months observation period. The effect on lymphocytes was apparent by 3 days after exposure, stabilizing at approx. 50% of pre-exposure values for about 7 months, with a return to pre-exposure levels in the following 4 y. There was a progressive and significant (P < 0.001) decline in platelet counts during the 52-months postexposure period. The pattern of response in erythrocyte parameters was complex. Immediately after the accident, these values were less than the pre-exposure mean level; they gradually increased (P < 0.001) for approx. 2 y and then began a progressive decline (P < 0.001)

  2. Percutaneous Nephrolithotomy for Kidney Stones in Patients with Hematological Malignancy

    Directory of Open Access Journals (Sweden)

    Baris Kuzgunbay

    2016-07-01

    Full Text Available Aim: To define the alterations in the outcomes of percutaneous nephrolithotomy (PNL operations for kidney stones in patients with history of hematological malignancy (HM. Material and Method: Between 2000 and 2013, 1700 adult patients underwent PNL for the treatment of kidney stones in our institution. Four of these patients had a history of HM and considered to be HM group (n=4. Ten elderly (>65 years patients who had no history of operation, HM or any other co-morbide diseases were chosen as the control group (n=10. Surgical parameters, success rates, additional treatments and complications were evaluated. Results: Statistical analyses showed no significant differences between HM and control group according to stone area, operation time, fluoroscopy time, hospitalization time, %u2206Hb, blood transfusion rates and INR values (p>0.05. Statistical analyses revealed no significant differences between HM and control groups according to the success rates (p=0.470. Statistical analyses revealed no significant difference between groups for additional treatment requirements (p=0.882. No major perioperative complication was seen in both of the groups. Discussion: The treatment of kidney stone disease by PNL in patients with hematological malignancy is feasible, safe and effective. However, close cooperation with the Hematology Department before the operation is mandatory.

  3. Determinants of hematology-oncology trainees' postfellowship career pathways with a focus on nonmalignant hematology

    Science.gov (United States)

    Jenkins, Sarah; Mikhael, Joseph; Gitlin, Scott D.

    2018-01-01

    Nonmalignant hematologic conditions are extremely prevalent and contribute significantly to the global burden of disease. The US health care system may soon face a shortage of specialists in nonmalignant hematology. We sought to identify factors that lead hematology-oncology fellows to pursue (or not to pursue) careers in nonmalignant hematology. Cross-sectional, web-based survey distributed to 149 graduates of a hematology-oncology fellowship program at a large academic medical center between 1998 and 2016. Eighty-six out of 149 graduates responded (57.7%); most (59 [68.6%]) practice at an academic medical center. Respondents spend a mean of 61% of their time in clinical practice, 23.7% conducting research, 5.2% in education, and 5.2% in administration. Those in clinical practice spend a mean of 52.1% of their time in solid tumor oncology, 37.5% in hematologic malignancies, and 10% in nonmalignant hematology; only 1 spent >50% of time practicing nonmalignant hematology. Factors most significantly affecting choice of patient population included clinical experience during fellowship and intellectual stimulation of the patient population/disease type. Factors that could have most significantly influenced a decision to spend more time in nonmalignant hematology included increased exposure/access to role models and mentors and opportunities for better career growth/advancement. Fellowship graduates spend >50% of their time in clinical practice, but almost none spend a significant amount of time practicing nonmalignant hematology. Given the growing number of patients with nonmalignant hematologic conditions and a possible future provider shortage, medical trainees should be encouraged to pursue careers in nonmalignant hematology. PMID:29463548

  4. Hematological aftermath of the radiation accident in Istanbul.

    Science.gov (United States)

    Engin, Velittin Selcuk; Tufan, Fatih; Kalayoglu Besisik, Sevgi; Engin, Gulgun; Ozturk, Mustafa; Ersoy, Suleyman

    2015-01-01

    The effects of radiation exposure are long-lasting. Long-term monitoring is imperative to diagnose late effects and improve our far-sightedness about possible events in the future. A radiation accident occurred in Istanbul in 1998 that resulted in mild to moderate acute radiation syndrome (ARS). In this study we aimed to investigate the changes in hematological parameters at the long-term follow-up of ARS patients. Ten adults were hospitalized after exposure to a 60Co source. Seven were diagnosed as having ARS and had severe and symptomatic pancytopenia. All of the exposed people recovered following intensive treatment. Treatment was supportive with transfusion, granulocyte-colony stimulating factor, and anti- infective management covering antifungal agents. Patients were closely monitored. Nine years after the accident, the initial and follow-up complete blood count examinations and peripheral blood smears (PBS) were comparatively evaluated by an experienced hematologist. The hematological laboratory values of the patients on admission, after treatment, and nine years after the accident were documented and compared. Biodosimetric analysis revealed that whole-body doses ranged from 1-1.9 Gy. All subjects have shown complete recovery of the hematological laboratory values after treatment. All but one of the subjects showed complete blood cell recovery. The improvement of the blood cell count of the excepted patient stalled at a mildly reduced level and his bone marrow was still hypocellular nine years after the accident; however, no malignant changes were detected. Values at admission were significantly different compared with post treatment and present values of all patients. Post treatment and follow-up values were similar. One of the patients died of lung cancer. None of the patients developed hematological malignancy. In this study, the recovery from ARS was complete after treatment. The small population, short follow-up period, and the relatively small doses

  5. DCB - Cancer Immunology, Hematology, and Etiology Research

    Science.gov (United States)

    Part of NCI’s Division of Cancer Biology’s research portfolio, studies supported include the characterization of basic mechanisms relevant to anti-tumor immune responses and hematologic malignancies.

  6. Immunological and hematological reference values for apparently ...

    African Journals Online (AJOL)

    admin

    negative adults in Bahir Dar Town,. Ethiopia. Bayeh Abera1, Atnaf Alem2, Ambahun Cherenet2, Mulugeta Kibret3. Abstract. Background: Immunological and hematological reference values differ among different human beings with respect to.

  7. Epidemiology of preoperative hematologic assessment of children cared for in a pediatric emergency department.

    Science.gov (United States)

    Woll, Christopher; Smith, P Brian; Fox, James Walter

    2018-01-01

    To assess frequency of preoperative hematologic testing in a tertiary care pediatric emergency department (PED) and how often these values predict clinical outcome or change management decisions. Single-center retrospective cohort study in a tertiary-care children's hospital PED. Patients 0-18years old, presenting between July 1, 2009-July 1, 2011, ultimately undergoing a surgical procedure within 48h of presentation were included. Patients were defined as having "preoperative" hematologic assessment if these studies were performed solely because the child was going to the operative suite. Patients who met trauma team activation criteria, underwent neurosurgical procedures, or had laboratory studies performed prior to PED arrival were excluded. The primary outcome was the prevalence of preoperative laboratory assessment. 528 children were included, of whom 301 (57%) underwent preoperative hematologic laboratory evaluations. Of these 301 patients, 115 (38%) had abnormal hematologic parameters, and only 3 (1%) of these patients had their perioperative management changed. One additional child had intraoperative bleeding that required blood products but did not undergo preoperative hematologic assessment. All four children had medical histories that would have identified their risk for perioperative bleeding events. Preoperative hematologic laboratory assessment occurs frequently in children initially cared for in a tertiary care pediatric emergency department who subsequently undergo operative interventions. Although age-based abnormal hematologic values are often found, rarely are these abnormalities clinically significant. This study suggests that children cared for in a PED without a history concerning for an increased risk of perioperative bleeding does not require preoperative hematologic assessment. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Normal ranges and test-retest reproducibility of flow and velocity parameters in intracranial arteries measured with phase-contrast magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Correia de Verdier, Maria; Wikstroem, Johan [Uppsala University Hospital, Department of Radiology, Uppsala University, Uppsala (Sweden)

    2016-05-15

    The purpose of the present study was to investigate normal ranges and test-retest reproducibility of phase-contrast MRI (PC-MRI)-measured flow and velocity parameters in intracranial arteries. Highest flow (HF), lowest flow (LF), peak systolic velocity (PSV), and end diastolic velocity (EDV) were measured at two dates in the anterior (ACA), middle (MCA), and posterior (PCA) cerebral arteries of 30 healthy volunteers using two-dimensional PC-MRI at 3 T. Least detectable difference (LDD) was calculated. In the left ACA, HF was (mean (range, LDD)) 126 ml/min (36-312, 59 %), LF 61 ml/min (0-156, 101 %), PSV 64 cm/s (32-141, 67 %), and EDV 35 cm/s (18-55, 42 %); in the right ACA, HF was 154 ml/min (42-246, 49 %), LF 77 ml/min (0-156, 131 %), PSV 75 cm/s (26-161, 82 %), and EDV 39 cm/s (7-59, 67 %). In the left MCA, HF was 235 ml/min (126-372, 35 %), LF 116 ml/min (42-186, 48 %), PSV 90 cm/s (55-183, 39 %), and EDV 46 cm/s (20-66, 28 %); in the right MCA, HF was 238 ml/min (162-342, 44 %), LF 120 ml/min (72-216, 48 %), PSV 88 cm/s (55-141, 35 %), and EDV 45 cm/s (26-67, 23 %). In the left PCA, HF was 108 ml/min (42-168, 54 %), LF 53 ml/min (18-108, 64 %), PSV 50 cm/s (24-77, 63 %), and EDV 28 cm/s (14-40, 45 %); in the right PCA, HF was 98 ml/min (30-162, 49 %), LF 49 ml/min (12-84, 55 %), PSV 47 cm/s (27-88, 59 %), and EDV 27 cm/s (16-41, 45 %). PC-MRI-measured flow and velocity parameters in the main intracranial arteries have large normal ranges. Reproducibility is highest in MCA. (orig.)

  9. Effects of Supplemental Chromium Source and Concentration on Growth, Carcass Characteristics, and Serum Lipid Parameters of Broilers Reared Under Normal Conditions.

    Science.gov (United States)

    Zheng, Cancai; Huang, Yanling; Xiao, Fang; Lin, Xi; Lloyd, Karen

    2016-02-01

    An experiment was conducted to investigate the effects of dietary chromium (Cr) source and concentration on growth performance, carcass traits, and some serum lipid parameters of broilers under normal rearing conditions for 42 days. A total of 252 1-day-old Cobb 500 commercial female broilers were randomly allotted by body weight (BW) to one of six replicate cages (six broilers per cage) for each of seven treatments in a completely randomized design involved in a 2 × 3 factorial arrangement of treatments with three Cr sources (Cr propionate (CrPro), Cr picolinate (CrPic), Cr chloride (CrCl3)) and two concentrations of added Cr (0.4 and 2.0 mg of Cr/kg) plus a Cr-unsupplemented control diet. The results showed that dietary Cr supplementation tended to increase the breast muscle percentage compared with the Cr-unsupplemented control group (P = 0.0784), while Cr from CrPic tended to have higher breast muscle percentage compared with Cr from CrCl3 (P = 0.0881). Chromium from CrPic also tended to increase the breast intramuscular fat (IMF) compared with Cr from CrCl3 (P = 0.0648). In addition, supplementation of 0.4 mg/kg Cr tended to decrease low-density lipoprotein cholesterol (LDL-C) (P = 0.0614). Compared with the control group, broilers fed Cr-supplemented diets had higher triglyceride (TG) (P = 0.0129) regardless of Cr source and Cr concentration. Chromium from CrPro and CrPic had lower total cholesterol (TC) compared with Cr from CrCl3 (P = 0.0220). These results indicate that dietary supplementation of Cr has effects on carcass characteristics and serum lipid parameters of broilers under normal rearing conditions, while supplementation of organic Cr can improve carcass characteristics and reduce the cholesterol content in serum.

  10. effects of dietary xylopia aethiopica on hematological parameters ...

    African Journals Online (AJOL)

    honey

    2014-03-31

    Mar 31, 2014 ... The desired amount of the powdered Xylopia aethiopica was measured using a sensitive digital weighing balance (Zohaus, Model CS 200, N.J. USA). ... red blood cell count, white blood cell count, white blood cell differential, platelet, ESR and PCV estimation. 2.5ml of blood from each animal was put into ...

  11. Impact of nickel (Ni) on hematological parameters and behavioral ...

    African Journals Online (AJOL)

    The fish were exposed to different concentrations (0, 6, 9, 12, 15 and 18 mg/l) of nickel sulphate using standard screening procedure. The mortality rate of the experimental fish was increased with increase in concentration of nickel. The 96 h median lethal concentration (96 h LC50) was 12.44 mg Ni/L using the logarithmic ...

  12. Effects of Dietary Xylopia aethiopica on Hematological Parameters ...

    African Journals Online (AJOL)

    It is concluded that the whole fruits of Xylopia aethiopica exhibited positive effects on blood cell indices and properties, probably by virtue of its rich iron content, and that its reduction in serum total cholesterol is a welcome development as an antihyperlipidemia against the cardiovascular risk factors associated with elevated ...

  13. correlation of hematological and biochemical parameters to severity ...

    African Journals Online (AJOL)

    2015-07-06

    Jul 6, 2015 ... Medicine, Katuri Medical College, Guntur. 2Corresponding Author,Professor, Department of Physiology, Katuri Medical College. Katuri. Medical College & Hospital, Guntur - 522 019,. Andhra Pradesh, India. e-mail: ... infection alone without other complicating illness may produce anemia in some patients.

  14. Alterations of diffusion tensor MRI parameters in the brains of patients with Parkinson's disease compared with normal brains: possible diagnostic use

    International Nuclear Information System (INIS)

    Lu, Chin-Song; Weng, Yi-Hsin; Lin, Wey-Yil; Ng, Shu-Hang; Cheng, Jur-Shan; Wai, Yau-Yau; Chen, Yao-Liang; Wang, Jiun-Jie

    2016-01-01

    To investigate the diagnostic performance of diffusion tensor imaging in patients with Parkinson's disease (PD). We examined a total of 126 PD patients (68 males/58 females, mean age: 62.0 ±7.6 years) and 91 healthy controls (43 males/48 females, mean age: 59.8 ±7.2 years). Images were acquired on a 3 Tesla magnetic resonance scanner. The Camino software was used to normalize and parcellate diffusion-weighted images into 90 cerebral regions based on the automatic anatomical labelling template. The minimum, median, and maximum values of the mean/radial/axial diffusivity/fractional anisotropy were determined. The diagnostic performance was assessed by receiver operating characteristic analysis. The associations of imaging parameters with disease severity were tested using Pearson's correlation coefficients after adjustment for disease duration. Compared with healthy controls, PD patients showed increased diffusivity in multiple cortical regions that extended beyond the basal ganglia. An area under curve of 85 % was identified for the maximum values of mean diffusivity in the ipsilateral middle temporal gyrus. The most significant intergroup difference was 26.8 % for the ipsilateral inferior parietal gyrus. The measurement of water diffusion from the parcellated cortex may be clinically useful for the assessment of PD patients. (orig.)

  15. The Effect of Normally Consumed Amounts of Sucrose or High Fructose Corn Syrup on Lipid Profiles, Body Composition and Related Parameters in Overweight/Obese Subjects

    Directory of Open Access Journals (Sweden)

    Joshua Lowndes

    2014-03-01

    Full Text Available The American Heart Association (AHA has advocated that women and men not consume more than 100 and 150 kcal/day, respectively, from added sugars. These levels are currently exceeded by over 90% of the adult population in the United States. Few data exist on longer-term metabolic effects when sucrose and High Fructose Corn Syrup (HFCS, the principal sources of added dietary sugars, are consumed at levels typical of the general population. Sixty five overweight and obese individuals were placed on a eucaloric (weight stable diet for 10-weeks, which incorporated sucrose- or HFCS-sweetened, low-fat milk at 10% or 20% of calories in a randomized, double-blinded study. All groups responded similarly (interaction p > 0.05. There was no change in body weight in any of the groups over the 10-week study, or in systolic or diastolic blood pressure. Likewise, there were no changes in total cholesterol, triglycerides, low-density lipoprotein (LDL, or apolipoprotein B (Apo B. We conclude that (1 when consumed as part of a eucaloric diet fructose—when given with glucose (as normally consumed does not promote weight gain or an atherogenic lipid profile even when consumed at two to four times the level recently recommended by the AHA. (2 There were no differences between HFCS and sucrose on these parameters.

  16. Alterations of diffusion tensor MRI parameters in the brains of patients with Parkinson's disease compared with normal brains: possible diagnostic use

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Chin-Song; Weng, Yi-Hsin; Lin, Wey-Yil [Chang Gung Memorial Hospital, Division of Movement Disorders,Department of Neurology, Taoyuan (China); Chang Gung Memorial Hospital, Neuroscience Research Center, Taoyuan (China); Chang Gung University, School of Traditional Chinese Medicine, Taoyuan (China); Ng, Shu-Hang [Chang Gung Memorial Hospital, Department of Medical Imaging and Intervention, Linkou (China); Chang Gung University, Department of Medical Imaging and Radiological Sciences, Taoyuan County (China); Cheng, Jur-Shan [Chang Gung University, Clinical Informatics and Medical Statistics Research Center,College of Medicine, Taoyuan (China); Wai, Yau-Yau [Chang Gung Memorial Hospital, Department of Medical Imaging and Intervention, Keelung (China); Chen, Yao-Liang [Chang Gung Memorial Hospital, Department of Medical Imaging and Intervention, Linkou (China); Chang Gung Memorial Hospital, Department of Medical Imaging and Intervention, Keelung (China); Wang, Jiun-Jie [Chang Gung Memorial Hospital, Neuroscience Research Center, Taoyuan (China); Chang Gung Memorial Hospital, Department of Medical Imaging and Intervention, Linkou (China); Chang Gung University, Department of Medical Imaging and Radiological Sciences, Taoyuan County (China); Chang Gung University / Chang Gung Memorial Hospital, Linkou, Medical Imaging Research Center, Institute for Radiological Research, Taoyuan (China)

    2016-11-15

    To investigate the diagnostic performance of diffusion tensor imaging in patients with Parkinson's disease (PD). We examined a total of 126 PD patients (68 males/58 females, mean age: 62.0 ±7.6 years) and 91 healthy controls (43 males/48 females, mean age: 59.8 ±7.2 years). Images were acquired on a 3 Tesla magnetic resonance scanner. The Camino software was used to normalize and parcellate diffusion-weighted images into 90 cerebral regions based on the automatic anatomical labelling template. The minimum, median, and maximum values of the mean/radial/axial diffusivity/fractional anisotropy were determined. The diagnostic performance was assessed by receiver operating characteristic analysis. The associations of imaging parameters with disease severity were tested using Pearson's correlation coefficients after adjustment for disease duration. Compared with healthy controls, PD patients showed increased diffusivity in multiple cortical regions that extended beyond the basal ganglia. An area under curve of 85 % was identified for the maximum values of mean diffusivity in the ipsilateral middle temporal gyrus. The most significant intergroup difference was 26.8 % for the ipsilateral inferior parietal gyrus. The measurement of water diffusion from the parcellated cortex may be clinically useful for the assessment of PD patients. (orig.)

  17. Age Related Changes in Hematological Values of Myanmar Local Puppies

    Directory of Open Access Journals (Sweden)

    Thandar Oo

    2017-10-01

    Full Text Available The hematological parameters were used to monitor the health status and its components also changed according to the ages. However, there were no reports for this issues in Myanmar local dogs. Thus, this study was carried out to investigate the age-related changes on the hematological parameters of local puppies in Myanmar. Ten local puppies with the age of 2-3 month old were used in this experiment, which was lasted for 8 weeks.The daily clinical examinations were conducted throughout the entire experimental period for general health check-up. Haematological parameters (Total WBC count and its differential counts, and RBC, HCT, MCV, HGB, MCH, MCHC and platelets were measured bi-weekly with Abacus Vet-5 automate haematology analyser. According to the results, the total WBC and eosinophil counts were not significantly different (P>0.05, while lymphocytes, monocytes, neutrophils and basophils were significantly different (P0.05 throughout the experimental periods. Thus, the age-related changes were observed on cell counts of lymphocytes, monocytes, neutrophils, basophils in Myanmar local puppies.

  18. The european hematology association roadmap for european hematology research : A consensus document

    NARCIS (Netherlands)

    A. Engert (Andreas); C.L. Balduini (Carlo); A. Brand (Anneke); B. Coiffier (Bertrand); C. Cordonnier (Charlotte); H. Döhner (Hartmut); De Wit, T.D. (Thom Duyvené); Eichinger, S. (Sabine); W.E. Fibbe (Willem); Green, T. (Tony); De Haas, F. (Fleur); A. Iolascon (Achille); T. Jaffredo (Thierry); F. Rodeghiero (Francesco); G. Salles (Gilles); J.J. Schuringa (Jan Jacob)

    2016-01-01

    textabstractThe European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European

  19. Hematologic responses to deferasirox therapy in transfusion-dependent patients with myelodysplastic syndromes

    Science.gov (United States)

    Gattermann, Norbert; Finelli, Carlo; Della Porta, Matteo; Fenaux, Pierre; Stadler, Michael; Guerci-Bresler, Agnes; Schmid, Mathias; Taylor, Kerry; Vassilieff, Dominique; Habr, Dany; Marcellari, Andrea; Roubert, Bernard; Rose, Christian

    2012-01-01

    Background Reductions in transfusion requirements/improvements in hematologic parameters have been associated with iron chelation therapy in transfusion-dependent patients, including those with myelodysplastic syndromes; data on there reductions/improvements have been limited to case reports and small studies. Design and Methods To explore this observation in a large population of patients, we report a post-hoc analysis evaluating hematologic response to deferasirox in a cohort of iron-overloaded patients with myelodysplastic syndromes enrolled in the Evaluation of Patients’ Iron Chelation with Exjade® (EPIC) study using International Working Group 2006 criteria. Results Two-hundred and forty-seven, 100 and 50 patients without concomitant medication for myelodysplastic syndromes were eligible for analysis of erythroid, platelet and neutrophil responses, respectively. Erythroid, platelet and neutrophil responses were observed in 21.5% (53/247), 13.0% (13/100) and 22.0% (11/50) of the patients after a median of 109, 169 and 226 days, respectively. Median serum ferritin reductions were greater in hematologic responders compared with non-responders at end of study, although these differences were not statistically significant. A reduction in labile plasma iron to less than 0.4 μmol/L was observed from week 12 onwards; this change did not differ between hematologic responders and non-responders. Conclusions This analysis suggests that deferasirox treatment for up to 1 year could lead to improvement in hematologic parameters in some patients with myelodysplastic syndromes. PMID:22419577

  20. Serum biochemistry and hematology values and hemoglobin electrophoresis in Persian squirrels (Sciurus anomalus).

    Science.gov (United States)

    Asadi, Farzad; Rostami, Amir; Asadian, Peyman; Pourkabir, Malihe

    2007-06-01

    Serum biochemical and hematologic parameters are important in the management of game species in Iran, such as Persian squirrels. The purpose of this study was to establish baseline serum chemistry and hematology values in Persian squirrels (Sciurus anomalus) and describe blood cell morphology and the electrophoretic pattern of hemoglobin. Blood samples were collected from 30 Persian squirrels (Sciurus anomalus) maintained in captivity in the Tehran Zoo. Blood was placed into EDTA and serum clot tubes and analyzed using standard manual hematology and biochemical techniques. Hemoglobin electrophoresis was done on cellulose acetate paper strips. Minimum, maximum, and median values were obtained for 11 hematologic and 12 serum chemistry parameters. Hypersegmented neutrophils were observed frequently. We did not find basophils or band neutrophils. Hemoglobin electophoresis resulted in a band slightly anodal to that of human hemoglobin A. Biochemical and hematologic values in Persian squirrels were comparable to those of related species, and may be used as a standard profile for healthy Persian squirrels kept in captivity.

  1. Investigation of transfer parameters from the radiochromium on erythrocyte kinetic

    International Nuclear Information System (INIS)

    Tavares, J.L.M.

    1980-01-01

    This study analyzes and interprets results of destruction and survival data from 51 Cr labeled red cells to the more common and realistic situations for diagnostic applications in clinical and nuclear hematology. The destructive process and the deviation of the cell system from the equilibrium state can be conveniently studied in terms of the disappearance rate of labeled red blood cells, using some transfer parameters. The investigation was concentrated on selection and study of a mathematical model to describe significantly the elimination process and to improve and simplify the computational analysis of data in chromium erythrokinetics from patients and normal individuals for control. (author)

  2. Value of innovation for hematologic malignancies.

    Science.gov (United States)

    Monia, Marchetti

    2016-01-01

    Several novel drugs are dramatically improving both lifespan and quality-of-life of patients with blood cancers. Prolonged disease duration and increased treatment costs for hematologic malignancies impose a relevant economic burden onto healthcare services, despite the low incidence of blood cancers. Therefore, an appropriate paradigm for valuing 'innovation' is urgently required in order to refine pricing and reimbursement decisions. Cost-per-QALY-gained is still the standard metric for assessing the 'incremental' value of new drugs; however, the high number of 'comparator' therapies and the huge variety of treatment sequences make plain two-treatment comparisons sub-optimal, while multiple-treatment and multiple-sequence comparisons require complex and less-transparent decision models. A repository of standard backbones for decision models might allow benchmarking and comparability among cost-effectiveness analyses; however, an international effort is required to build it up. Deontology recommends that hematologists act in optimizing healthcare resources while preserving patient-physician alliance, but clinical practice guidelines do not support doctors in balancing cost against clinical outcomes. Decision models of chronic blood cancers unexpectedly proved that cost might be an appropriate value for innovation if treatments avoided severe toxicity and further lines of treatments, despite the eventually long duration of treatment and the competing risk of death due to comorbidity and old age. The improved transparency of decision models allows sharing of relevant structural and analytic parameters (i.e., time horizon, comparator treatments, hierarchy of end-point, assumptions, source of data, sub-group analyses) by stakeholders, physicians and patients, making health economics a noble 'translator' of values for innovation.

  3. Oral microflora in children with hematologic malignancies

    Directory of Open Access Journals (Sweden)

    M. F. Vecherkovskaya

    2015-06-01

    Full Text Available The goal was a comprehensive study of oral microflora in healthy children and those with hematologic malignancies, based on the analysis of mixed microbial biofilms composition, isolation and identification of new previously unknown microorganisms. The material was obtained in children with hematological diseases in remission, 2–10 years aged, and for the control group from St. Petersburg schoolchildren and in kindergartens. We used microbiological, biochemical and molecular genetic methods, including electron microscopy, proteomic analysis, sequencing and complete genome annotation. Microorganisms of 23 genera isolated as pure cultures and identified by biochemical activity from mixed microbial biofilm derived from saliva of healthy and sick children. In microflora of children with hematologic malignancies a previously unknown type of streptococci with a large number of antibiotic resistance genes was revealed. Differences in oral microbiota composition of healthy children and children with hematological diseases in remission were revealed. The microbiota of children with hematologic malignancies contains more genes controlling antibiotic resistance. Also, it was observed previously unknown bacterium of the genus Streptococcus.

  4. EFFECT OF A VITAMIN E AND SELENIUM ORAL SUPPLEMENTATION ON HEMATOLOGICAL PARAMETERS, MUSCULAR INJURIES MARKERS ENZYMES AND PEROXIDATION INDICES OF BIOMOLECULES IN SHOW JUMPING HORSES EFEITO DA SUPLEMENTAÇÃO COM VITAMINA E E SELÊNIO SOBRE O QUADRO HEMATOLÓGICO, ENZIMAS MARCADORAS DE LESÃO MUSCULAR E ÍNDICE DE PEROXIDAÇÃO DE BIOMOLÉCULAS EM EQUINOS SUBMETIDOS À ATIVIDADE DE SALTO

    Directory of Open Access Journals (Sweden)

    Domingos Cachineiro Rodrigues Dias

    2009-09-01

    Full Text Available Among the main physiopathological mechanisms in the appearance of injuries related to the physical exercise, the involvement of free radicals is very important, mainly oxygen reactive species. They can lead to hematologicaland biochemical alterations related to injuries mediated by the physical effort. For the prevention and treatment of such conditions, the use of antioxidant supplementation, like ?-tocopherol (vitamin E and selenium has been considered for training animals and competitions of high impact. The aim of this research was to evaluate the influence of vitamin E and selenium oral supplementation on hematological parameters, the seric activity of muscular enzymes and the level of substances derived from oxidative stress on erythrocyte membranes of horses normally trained and submitted to a show jumping test. For this purpose, 20 horses with these characteristics were divided in two groups. One of them (GT received oral supplementation of vitamin E (2500 mg/day and selenium (3 mg/day for 45 days and another one was kept as control (GC. Both groups were submitted to a standardized test of show jumping and samples of blood were collected in rest, immediately after, and 6, 12 and 24 hours after the end of the activity. The constituents of the hemogram, the seric activity of enzymes CK, AST, and LDH, and the level of an oxidative stress marker (TBARS in erythrocyte membranes were evaluated. The number of erythrocytes and leukocytes returned to basal levels in GT after 6 hours. In GC, such values returned to rest levels between 12 and 24 hours. Despite the observed differences in some hematological parameters dynamics between the two groups, it was not possible to determine antioxidant activity in GT. These observations demonstrate the need for more studies to understanding oxidative stress effects on athletics horses and the use of antioxidants for these animal’s diets.

    KEY WORDS: Exercise, horse, oxidative stress, selenium

  5. Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

    Directory of Open Access Journals (Sweden)

    Nahid Ahmadi

    2018-02-01

    Full Text Available The widespread use of pesticides in agriculture represents a threat to the human populations exposed to them. In this cross-sectional study, the hematological and biochemical parameters, plasma cholinesterase (PChE activity, oxidative stress, genotoxicity, and NAD(PH: quinone oxidoreductase 1 (NQO1 C609T polymorphism were measured in 100 greenhouse workers occupationally exposed to pesticide mixture and 104 normal healthy controls. There was a decrease in erythrocytes (5.45%, p = 0.026 and hemoglobin (3.26%, p = 0.025, and an increase in mean corpuscular hemoglobin (3.54%, p = 0.013 in the exposed workers. Sprayers showed a reduction in PChE (23% and GSH (50% levels, and an increase in lipid peroxidation (LPO (55%, protein carbonyl (145%, Superoxide dismutase activity (61%, and total antioxidant capacity (35% (p < 0.001 for all parameters but LPO: p = 0.009. Genotoxicity parameters were significantly high in the exposed cases (for all parameters: p < 0.001 but tail length: p = 0.002. There was a significant correlation between oxidative stress and genotoxicity parameters, and also between these biomarkers and PChE activity. The NQO1 C609T polymorphism was not significantly associated with studied biomarkers. The findings indicate that occupational exposure to a mixture of pesticides can induce hematotoxicity, oxidative stress, and genotoxicity in greenhouse workers.

  6. Clinical analysis of surgical treatment in patients with senile cataract and hematologic disease

    Directory of Open Access Journals (Sweden)

    Wan-Hui Wang

    2013-11-01

    Full Text Available AIM: To evaluate the clinical efficacy of cataract extraction and intraocular lens implantation in patients with senile cataract and hematologic disease. METHODS: Forty patients(58 eyeswith senile cataract and hematologic disease underwent cataract extraction and intraocular lens implantation. Preoperative assessment included blood coagulation testing, leukocyte classification, and bone marrow examination. Appropriate preoperative treatments were administered to patients with senile cataract and thrombocytopenia, leukopenia, anemia, and/or pancytopenia(identified through blood testing. Thirty patients with senile cataract and normal routine bloodwork findings, with ages similar to those of patients in the study group, were randomly recruited as a control group. Postoperative corrected visual acuity and intraoperative and postoperative complications were recorded. The causes of hematologic disease in patients in the study group were recorded. Blood clotting data were compared between groups using t-tests. RESULTS: Plasma prothrombin time and activated partial thromboplastin time did not differ significantly between the study and control groups(P=0.379 and P=0.945, respectively. Surgery improved corrected visual acuity in all patients(>0.4 in 56 〖96.55%〗 eyes. No postoperative infection, bleeding or fever occurred. The etiology of hematological disease was unknown in some patients; most patients had diabetes mellitus, hypertension, cirrhosis, and/or kidney disease. CONCLUSION: With adequate perioperative treatment, patients with senile cataract and hematological disease can safely undergo cataract operation and intraocular lens implantation, with good outcomes.

  7. Hematology and serum chemistry of the island spotted skunk on Santa Cruz Island.

    Science.gov (United States)

    Crooks, Kevin R; Garcelon, D K; Scott, Cheryl A; Wilcox, Jeffery T; Timm, Steven F; Van Vuren, Dirk H

    2003-04-01

    We determined serum biochemistry and hematologic values for island spotted skunks (Spilogale gracilis amphiala) on Santa Cruz Island (California, USA). Samples were collected from island spotted skunks chemically restrained with ketamine hydrochloride and acepromazine in August 1999 (dry season) and from skunks manually restrained in August 2000 (dry season) and January 2001 (wet season). One parameter, glucose, significantly differed with season, with higher levels during the wet season. Serum chemistry and hematologic profiles suggest that method of restraint (manual or chemical), as well as other methodologic details, may influence blood characteristics in the island spotted skunk.

  8. Changes in the blood parameters of the Amazonian manatee (Trichechus inunguis after long-distance transportation - doi: 10.4025/actascibiolsci.v35i4.20081

    Directory of Open Access Journals (Sweden)

    Talita Laurie Lustosa do Carmo

    2013-07-01

    Full Text Available In this study we report the hematological, biochemical and hormonal parameters in a juvenile male Amazonian manatee measured before transport, immediately after transport, and during adaptation to a new facility. The animal was transported from Manaus, Amazonas, Brazil, to São Paulo, São Paulo, Brazil, (2,733 km within 6 hours. Among all blood parameters analyzed, we observed obvious neutrophilia, lymphopenia, and increases in the neutrophil/lymphocyte ratio and serum glucose and aspartate aminotransferase (AST levels, but these parameters subsequently returned to normal. These results suggest that transport and changes in the environment are temporary stressful events for Amazonian manatees. We, therefore, recommend monitoring the hematological and biochemical parameters before and after translocation to minimize the effects of handling stressors in this species

  9. Bacteremia and candidemia in hematological malignancies

    DEFF Research Database (Denmark)

    Hovgaard, D; Skinhøj, P; Bangsborg, Jette Marie

    1988-01-01

    171 episodes of bacteremia and candidemia in 142 patients were recorded during the period 1981-1985 in patients with hematological malignancies. Overall mortality, within 1 week of onset of bacteremia, was 20%. Increased mortality was found in patients with poor disease-prognosis (39%), with gran......171 episodes of bacteremia and candidemia in 142 patients were recorded during the period 1981-1985 in patients with hematological malignancies. Overall mortality, within 1 week of onset of bacteremia, was 20%. Increased mortality was found in patients with poor disease-prognosis (39...

  10. Survival From Childhood Hematological Malignancies in Denmark

    DEFF Research Database (Denmark)

    Erdmann, Friederike; Winther, Jeanette Falck; Dalton, Susanne Oksbjerg

    2016-01-01

    in children with hematological malignancies. RESULTS: Having siblings and increasing birth order were associated with reduced survival from acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Associations with AML were strongest and statistically significant. HRs of 1.62 (CI 0.85; 3.09) and 5...... with a hematological malignancy before the age of 20 years (N = 1,819) were followed until 10 years from diagnosis. Kaplan-Meier curves and Cox proportional hazards models estimating hazard ratios (HR) and 95% confidence intervals (CI) were used to assess the impact of family characteristics on overall survival...

  11. Multi-factor analysis on events related to hematological toxicity in 153Sm-EDTMP palliative therapy for skeletal metastases

    International Nuclear Information System (INIS)

    Zhan Hongwei; Yu Xiaoling; Ye Xiaojuan; Bao Chengkan; Sun Da; He Gangqiang

    2006-01-01

    Objective: To investigate the clinical factors related to hematological toxicity induced by intravenous samarium-153 ethylenediaminetetramethylene phosphonic acid ( 153 Sm-EDTMP) treatment. Methods A total of 206 patients with bony metastases treated with 153 Sm-EDTMP were retrospectively analyzed. Logistic regression (SPSS 10.0 for Windows) and correlation analysis were used to evaluate the factors concerned. Results: Age of the patient, number of bone metastatic lesion, chemotherapy before 153 Sm-EDTMP therapy, concurrent radiotherapy and repeat-times of 153 Sm-EDTMP treatments were found the individual factors related to hematological toxicity. Chemotherapy before 153 Sm-EDTMP, concurrent radiotherapy, medication for normal blood counting and repeat-times of 153 Sm-EDTMP treatments were the hematological toxicity factors in multi-factor analysis. Conclusion: In 153 Sm-EDTMP therapy, several factors were found related to hematological toxicity suggesting more attention be paid to the change of blood cell counting after the palliative therapy. (authors)

  12. Effects of Watercress (Nasturtium nasturtium extract on selected immunological parameters of rainbow trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    K. Ahmadi

    2012-05-01

    Full Text Available Watercress (Nasturtium nasturtium is a medical plant containing diverse chemically-active substances with biological properties. The present study was conducted to investigate the immunomodulatory effects of watercress extract on immunological and hematological parameters of rainbow trout (Oncorhynchus mykiss. Fish were fed for 21 days with diet supplemented with 0.1% and 1% of watercress extract per 1 kg food and with a normal diet as control. Hematological parameters such as red blood cells (RBC and white blood cells (WBC, hematocrit (Hct, hemoglobin (Hb, RBC index like mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH and mean corpuscular hemoglobin concentration (MCHC as well as immunological parameters such as peroxidase, lysozyme and complement activities, total protein, albumin and globulin levels were measured after 21 days of watercress extract treatment. The results indicated that oral administration of 1 % watercress extract in fish may enhance some hematological and immunological parameters including Hb and MCHC, lysozyme and complement activities, total protein and globulin levels, compared to the controls after 21 days of experimental period. In conclusion, on the basis of these results, oral administration of watercress extract may be useful to improve fish’s immune system.

  13. Effect of Resistance Training on Hematological Blood Markers in Older Men and Women: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Florian Bobeuf

    2009-01-01

    Full Text Available The aim of this study was to examine the effects of resistance training on hematological blood markers in older individuals. Twenty-nine men and women participated to this study. Subjects were randomized in 2 groups: (1 control (n=13 and (2 resistance training (n=16. At baseline and after the intervention, subjects were submitted to a blood sample to determine their hematological profile (red blood cells, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, lymphocytes, monocytes, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width. At baseline, no difference was observed between groups. Moreover, we found no significant difference after the intervention on any of these markers. A 6-month resistance program in healthy older individuals seems to have no beneficial nor deleterious effects on hematological blood parameters. However, resistance training was well tolerated and should be recommended for other health purposes. Further studies are needed to confirm these results in a large population.

  14. Hematological, biochemical and ruminant parameters for diagnosis of left displacement of the abomasum in dairy cows from Southern Brazil Indicadores hematológicos, bioquímicos e ruminais no diagnóstico do deslocamento de abomaso à esquerda em vacas leiteiras do Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Felipe Cardoso de Cardoso

    2008-01-01

    Full Text Available The objective of this work was to evaluate hematological, biochemical and ruminant parameters for diagnosis and treatment of the left displaced abomasum (LDA in dairy cows, in the Plateau Region of Rio Grande do Sul, Brazil. Ruminant fluid, blood and urine samples were collected from 20 cows suffering LDA and from 20 healthy cows (control. The cows with LDA showed lower values of daily milk production, body weight and corporal condition score. The use of pH reagent strips showed to be functional in the field, when compared to a digital pH meter. Ruminant dynamics was damaged in cows affected by LDA, as it was evidenced by the higher reduction time of methylene blue. Serum values of lactate, beta-hydroxybutyrate, urea, albumin, free fatty acids and cholesterol shows to be auxiliary tools in the LDA characterization.O objetivo deste trabalho foi avaliar indicadores hematológicos, bioquímicos e ruminais no diagnóstico e tratamento do deslocamento de abomaso à esquerda (DAE em vacas leiteiras, na Região do Planalto do Rio Grande do Sul, Brasil. Foram coletadas amostras de líquido ruminal, sangue e urina de 40 animais, dos quais 20 vacas com DAE e 20 vacas clinicamente sadias utilizadas como grupo controle. Os animais com DAE, quando comparados ao grupo controle, apresentaram diminuição da produção de leite diária, do peso corporal e do escore condição corporal. A utilização de fitas reagentes para medição do pH ruminal demonstrou-se eficaz em campo, em comparação ao potenciômetro digital. A dinâmica ruminal apresentou-se prejudicada nos animais com DAE, o que foi evidenciado pelos valores aumentados do tempo de redução de azul de metileno. Os valores séricos de lactato, beta-hidroxibutirato, uréia, albumina, ácidos graxos livres e colesterol apresentam-se como ferramentas auxiliares na caracterização da doença.

  15. Níveis crescentes de parede de levedura sobre a resposta imune celular e perfil hematológico de frangos de corte Cellular immune response and hematological parameters of broilers from different age broiler breeders, fed with a sorghum meal with different yeast wall levels

    Directory of Open Access Journals (Sweden)

    Suelen Regina Ferreira

    2009-09-01

    Full Text Available Um experimento foi conduzido para avaliar o efeito de níveis crescentes de parede de levedura e idade das matrizes reprodutoras sobre o peso dos órgãos linfóides, a resposta imune celular e o perfil hematológico de frangos de corte. Foram utilizados 3.360 pintos de corte da linhagem Cobb, distribuídos em delineamento inteiramente casualisado, em esquema fatorial 2x5, mais dois controles, sendo duas idades de matrizes (34 e 57 semanas de idade e cinco níveis de suplementação de parede de levedura (zero, um, dois, três e quatro kg de parede de levedura/tonelada de ração. A idade das matrizes influenciou a resposta de todas as variáveis. A inclusão de 3kg de parede de levedura/tonelada de ração promoveu, na progênie de reprodutoras de 57 semanas, reação inflamatória mais intensa quando comparada a dieta controle, no entanto não houve aumento significativo no número de heterófilos e linfócitos circulantes. Conclui-se que a utilização da parede de levedura associada ao sorgo ou não em rações de frangos de corte ainda necessita de estudos complementares, que incluam, por exemplo, os componentes purificados da parede de levedura (MOS e ß-glucano.An experiment was carried out to evaluate the effect increasing levels of yeast wall and broiler breeders' age levels on lymphoid organs weight, cellular immune response and hematological parameters in broilers. A total of 3,360 Cobb broilers were allotted, in a completely randomized design and a 2x5 factorial arrangement, and two controls, compound of two broiler breeders age (34 and 57 weeks of age and five yeast wall levels (zero, one, two, three and four kg of yeast wall/ton of diet. Broiler breeders' age affected all studied variables. The inclusion of 3kg of yeast wall/ton of meal increased, at 57 weeks age broiler breeders, more intense inflammatory reaction when compared to control diet; however the circulated heterophils and lymphocytes numbers were not increased. In

  16. Fertility considerations in young women with hematological malignancies

    DEFF Research Database (Denmark)

    Jadoul, Pascale; Kim, S Samuel; Andersen, Claus Yding

    2012-01-01

    The need for practice guidelines for fertility preservation in young women with hematological malignancies has been increased. To develop recommendations, publications relevant to fertility preservation and hematological cancers were identified through a PubMed database search and reviewed...

  17. Verification and quality control of routine hematology analyzers

    NARCIS (Netherlands)

    Vis, J Y; Huisman, A

    2016-01-01

    Verification of hematology analyzers (automated blood cell counters) is mandatory before new hematology analyzers may be used in routine clinical care. The verification process consists of several items which comprise among others: precision, accuracy, comparability, carryover, background and

  18. No Gender Difference in Foreign Medical Students’ Hematology Clerkship

    OpenAIRE

    Li, Qing; Chen, Jianhui; Wan, Bo; Li, Xiaofan

    2018-01-01

    Hematology is a difficult aspect of medicine for students to learn. Students need hematology clerkship training to develop their clinical skills. The influence of gender on different teaching methods and performance in foreign medical students is still unknown, especially in hematology clerkship. To address this issue, we tested performance differences between foreign male and female students in clinical practice. Twenty-two foreign medical students attended a two-week hematology clerkship in...

  19. Biological Reference Interval for Hematological Profile of Umbilical Cord Blood: A Study Conducted at A Tertiary Care Centre in South India

    Science.gov (United States)

    Raj, Reddy S. Sudheer; Priyathersini, N.; Rajendran, Rithika; Rajendran, Rashmika; Ramadoss, Umalakshmi

    2015-01-01

    Introduction Umbilical cord blood (UCB) a source of hematopoietic stem cells, is also an acceptable sample to assess neonatal sepsis. Though reports are available for stem cell counts very minimal literature is available regarding hematologic parameters, which may vary on ethnicity. Aim To establish biological reference interval for hematological parameters of umbilical cord blood to guide neonatologists, hematopoietic stem cell transplant specialists and future analysis. Materials and Methods Prospective longitudinal study was done from January 2014 to April 2014 after ethics committee approval. UCB from 120 full term new borns of normal birth weight born out of uneventful pregnancy to mothers aged between 21 to 45 years with hemoglobin above 10g/dL were processed in Beckman Coulter LH780 analyzer for complete blood count and counter checked by peripheral smear. Results tabulated in Microsoft excel are analyzed using IBM SPSS statistics 16 software. Results Male to female ratio is 1:1.05. There is no difference in the values between males and females. When compared with few studies available, though many values are comparable a few values are not comparable. Conclusion This study can be a useful guide to neonatologists, hematopoietic stem cells transplant hematologists and future analysis. PMID:26557584

  20. Effects of maternal dexamethasone exposure on hematological ...

    African Journals Online (AJOL)

    Exposure to dexamethasone at LD 1-14 and 1-21 significantly (P<0.05) reduced RBC and platelet counts but it raised MCV and MCH relative to control. This study suggests that prenatal and lactational dexamethasone administration may affect the hematological indices in the male offspring. Keywords: Dexamethasone ...

  1. Comparison of clinical hematological changes under ...

    African Journals Online (AJOL)

    The objectives of this study were to compare the clinical hematological changes under anesthetization in Crucian carp (Carassius auratus auratus) due to treatment with local anesthetics. Our data indicate that the values declined significantly (P < 0.05) with dissolved oxygen amount after anesthetization, furthermore, the ...

  2. Hematological outcome in neonatal alloimmune hemolytic disease

    NARCIS (Netherlands)

    Rath, Mirjam Eva Aafke

    2013-01-01

    This thesis focuses on several aspects related to the hematological outcome of infants with hemolytic disease of the fetus and newborn (HDFN) due to red blood cell alloimmunization, including pathogenesis and management of the disease. The presence of leukocytopenie and thrombocytopenia support the

  3. Bacteremia and candidemia in hematological malignancies

    DEFF Research Database (Denmark)

    Bruun, B; Bangsborg, Jette Marie; Hovgaard, D

    1988-01-01

    The microorganisms isolated in 1981-1985 from 171 cases of septicemia in patients with hematological malignancies were on the whole the same as those found in 1970-1972. The distribution between species was also quite similar for the two periods except within staphylococci, where the isolation rate...

  4. Immunological and hematological reference values for apparently ...

    African Journals Online (AJOL)

    Background: Immunological and hematological reference values differ among different human beings with respect to sex, ethnicity, nutrition, altitude and health conditions. These could not be exceptional in the Ethiopian heterogeneous population. However, there are no nationally established reference values. Objective: ...

  5. Growth performance, carcass and hematological characteristics of ...

    African Journals Online (AJOL)

    Growth performance, carcass and hematological characteristics of rabbits fed graded levels of tiger nuts ( Cyperus esculentus ) ... (p>0.05) difference between treatments. Results demonstrated that (Cyperus esculentus) could be used up to 5% in rabbit's diets without adverse effect on the animals' performance and health.

  6. Hematology reference intervals for neonatal Holstein calves.

    Science.gov (United States)

    Panousis, Nikolaos; Siachos, Nektarios; Kitkas, Georgios; Kalaitzakis, Emmanouil; Kritsepi-Konstantinou, Maria; Valergakis, Georgios E

    2018-01-09

    Data regarding hematologic reference intervals (RI) for neonatal calves have not been published yet. The aims of this study were: a) to establish hematology RIs for neonatal Holstein calves, b) to compare them with the RIs for lactating cows, and c) to investigate the relationship of age and gender with the hematologic profile of calves. Two-hundred and fifty-four clinically healthy Holstein calves (1-9days old, from 30 farms) and 82 healthy Holstein cows (between 30 and 150days in milk, from 10 farms) were blood sampled once for a complete blood count evaluation, using the ADVIA 120 hematology analyzer. An additional blood sample was collected from each calf for serum total protein concentration measurement. RIs and age-related RIs were calculated with the Reference Value Advisor freeware. Comparisons between calves and cows and between male and female calves were performed with t-test or Mann-Whitney test. Red blood cell count (RBC), white blood cell count (WBC), neutrophil, lymphocyte and platelet counts in calves were higher, while mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were lower than in cows. Lymphocyte and platelets showed a notable increase through age. Finally, female calves had higher RBC, hematocrit and hemoglobin concentration than males. Age-specific RIs should be used for the interpretation of the complete blood count in Holstein calves. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Hematologic features among anemic Cameroonian pregnant women

    African Journals Online (AJOL)

    Introduction: iron deficiency anemia is the leading cause of anemia worldwide. It may also be the leading cause of anemia in pregnancy, although this has not yet been demonstrated in our country. The aim of the study was to describe hematologic features of Cameroonian anemic pregnant women. Methods: this cross ...

  8. Growth performance, hematological and serum biochemical ...

    African Journals Online (AJOL)

    A twelve (12) weeks feeding trial was carried out to determine the effect of varying dietary inclusions of Microdesmis puberula leaf meal (MPLM) on the growth performance, hematological indices and serum biochemical constituents of growing rabbits. Four grower rabbit diets were formulated to contain the leaf meal at ...

  9. Hematologic responses in patients with aplastic anemia treated with deferasirox: a post hoc analysis from the EPIC study

    Science.gov (United States)

    Lee, Jong Wook; Yoon, Sung-Soo; Shen, Zhi Xiang; Ganser, Arnold; Hsu, Hui-Chi; El-Ali, Ali; Habr, Dany; Martin, Nicolas; Porter, John B.

    2013-01-01

    Reports are emerging of hematologic responses associated with iron chelation therapy; however, studies are limited in aplastic anemia patients. Deferasirox reduced iron overload in aplastic anemia patients enrolled in the EPIC (Evaluation of Patients’ Iron Chelation with Exjade®) study (n=116). A post hoc analysis of hematologic responses was conducted on 72 patients with evaluable hematologic parameters (according to UK guideline criteria), 24 of whom received deferasirox without concomitant immunosuppressive treatment. Partial hematologic responses were observed in 11 of 24 (45.8%) patients; all became transfusion-independent. One patient had an additional platelet response and one patient had an additional platelet and hemoglobin response. Mean serum ferritin levels at end of study were significantly reduced in partial hematologic responders (n=11; −3948±4998 ng/mL; baseline 6693±7014 ng/mL; percentage change from baseline −45.7%; P=0.0029). In non-responders, the reduction in serum ferritin was less pronounced (n=13; −2021±3242 ng/mL; baseline 4365±3063 ng/mL; % change from baseline −27.6%; P=0.0171). Alongside reduction in iron overload, deferasirox may, therefore, improve hematologic parameters in a subset of aplastic anemia patients. Further investigation is required to elucidate the mechanisms involved. (Clinicaltrials.gov identifier: NCT00171821) PMID:23585526

  10. Fibrinogen measurements in plasma and whole blood: a performance evaluation study of the dry-hematology system.

    Science.gov (United States)

    Ogawa, Satoru; Tanaka, Kenichi A; Nakajima, Yasufumi; Nakayama, Yoshinobu; Takeshita, Jun; Arai, Masatoshi; Mizobe, Toshiki

    2015-01-01

    An accurate and rapid determination of fibrinogen level is important during hemorrhage to establish a timely hemostatic intervention. The accuracy of fibrinogen measurements may be affected by the specific methodology for its determination, fluid therapies, and anticoagulant agents. The dry-hematology method (DRIHEMATO®) is a novel approach to determine fibrinogen levels in plasma and whole blood based on thrombin-activated coagulation time. We hypothesized that plasma or whole blood fibrinogen level using the dry-hematology method would be similar to those measured with conventional plasma fibrinogen assays. Acquired hypofibrinogenemia was modeled by serial dilutions of blood samples obtained from 12 healthy volunteers. Citrated whole blood samples were diluted with either normal saline, 5% human albumin, or 6% hydroxyethyl starch to achieve 25%, 50%, and 75% volume replacement. The dry-hematology method, the Clauss method, the prothrombin time (PT)-derived method, determination of antigen levels, and thromboelastometric fibrin formation were compared in plasma or whole blood samples. The effect of heparin on each assay was examined (0 to 6 IU/mL). Comparisons of dry-hematology and other methods were also conducted using ex vivo samples obtained from cardiac surgical patients (n = 60). In plasma samples, there were no significant differences between dry-hematology and the Clauss method, while dry-hematology showed lower fibrinogen levels compared with PT-derived and antigen level methods. The dry-hematology method yielded acceptable concordance correlation coefficients (Pc) with the Clauss method, the PT-derived method, and fibrinogen antigen levels (Pc = 0.91-0.99). The type of diluents and heparin affected the results of the PT-derived method and thromboelastometric assay, but not the dry-hematology method. In cardiac surgical patients, the overall correlation in fibrinogen levels between dry-hematology and the other methods was comparable to the results from

  11. hematological and biochemical studies on the effect of some natural antioxidants pre-injection in irradiated rats

    International Nuclear Information System (INIS)

    Ashour, S.E.S.

    2011-01-01

    The present work was carried out in order to evaluate the biological activities of some natural antioxidants such as ziziphus and olive leaves extract as radioprotective agents on male albino rats treated with gamma irradiation. The results showed that the ethanolic extract of ziziphus and olive leaves have high content of phenolic and flavonoid compound. GC-MS showed that ethanolic extracts of ziziphus and olive leaves contains some important phenolic compounds include (Catechin, Chlorogenic,Zizyphine F, Luteolin and Succinylsulfathiazol), (Caffeic acid, Quercetin, Rutin, Diosmetine, Luteolin-7-glucoside and Oleuropein) respectively. γ-irradiation caused a significant decrease in body weight after 2 weeks as compared with control group. Administration of ethanolic extracts of olive and ziziphus leaves to normal rats exhibited a decrease in body weight after 2 weeks as compared with control group.The determination of different biological parameters showed a significant high level of ALT, AST, ALP, creatinine and urea in rat serum treated with gamma radiation. A significant depression in Hb, RBCs, HCT, MCV, WBC and PLT in rats after exposure to gamma radiation was noticed. The ethanolic extracts of ziziphus leaves and olive leaves application have been found to regulate the hematological parameters with narrow range around the normal level. A depressive effect of radiation was noticed on total protein, and albumin. Ethanolic extracts of olive and ziziphus leaves enhanced the accumulation of protein fraction.

  12. Establishment of hematological reference intervals for healthy adults in Asmara.

    Science.gov (United States)

    Siraj, Nejat; Issac, John; Anwar, Mohammed; Mehari, Yohannes; Russom, Shushan; Kahsay, Semere; Frezghi, Haben

    2018-01-22

    Clinical laboratory reference intervals used in a specific area should be derived from the local population as they are influenced by many factors. The purpose of this quantitative cross sectional study was to establish hematological reference intervals for healthy adults in Asmara and to determine whether the currently used reference interval do represent the adult population in the city. In addition, the established reference intervals were compared to findings from similar studies conducted in selected countries in Africa. There was a significant difference between males and females in the reference intervals for erythrocyte count, hemoglobin, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration and differential white blood cell count. All the evaluated hematological analytes were found to be higher in males than in females except for platelet count. The out of range percentage for the parameters extends from 3.5 to 46.7%; with red blood cell count having the lowest while mean cell volume having the highest out of range percentage. The results indicated that the currently used reference interval does not represent the population in Asmara and are different from those obtained elsewhere in Africa.

  13. Regulating hematology/oncology research involving human participants.

    Science.gov (United States)

    Kapp, Marshall B

    2002-12-01

    The conduct of hematology/oncology research, particularly clinical trials involving human participants, is an extensively regulated enterprise. Professionals in the specialty of hematology/oncology have important stakes in the success of biomedical research endeavors. Knowledge about and compliance strategies regarding the pertinent regulatory parameters are essential for avoiding negative legal repercussions for involved professionals. At the same time, there is a need to be aware of and actively resist the danger that strong [legal] protectionism might inadvertently result in undermining physician investigators' sense of personal moral responsibility in the conduct of human experiments. For all the limitations of that virtue in the protection of human subjects, it is surely not one that we would want medical scientists to be without [47]. Members of the potential participant pool, financial sponsors, and the general public must be convinced that everyone involved in the research enterprise is committed to operating within acceptable legal and ethical boundaries if the atmosphere of confidence and trust that is indispensable to the continued process and progress of investigation aimed at extending and improving quality of life for all of us in the future is to continue and flourish [48].

  14. IMMEDIATE REACTIONS TO MONOCLONAL ANTIBODIES IN CLINICAL HEMATOLOGY

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    Vasiliki KYRIAZI

    2016-12-01

    Full Text Available Monoclonal antibodies (MoAbs have been widely used in clinical hematology. As foreign macro-molecules, they can cause infusional reactions during the administration or within 24 hours after the infusion, which encompass a spectrum of mechanisms. Although most of these reactions are non-allergic, are often indistinguishable from true allergic reactions mediated by IgE immunoglobulins. The diagnosis is often challenging and relies mainly on clinical criteria. They occur during the first doses, soon after the initiation of treatment. The symptoms are usually well controlled by the immediate drug discontinuation or reduction of the infusion rate. The management remains largely supportive, consisting of oxygen, intravenous fluids, bronchodilators, antihistamines and steroids. Most of MoAb protocols recommend premedication with steroids and antihistamines and gradually escalating infusion rates. Increased medical and nursing vigilance is required and resuscitative equipment should always be readily available. These events affect patients' quality of life, leading to treatment delay or discontinuation and series of tests. The decision to rechallenge the treatment depends on severity grading, clinical parameters and treatment goals. This article provides an update of MoAbs used in clinical hematology. It summarizes the pathophysiology, the diagnostic approach, the preventive measures and treatment of MoAb-related reactions.

  15. Correlation of routine haematological parameters between normal maternal blood and the cord blood of healthy newborns in selected hospitals of karachi

    International Nuclear Information System (INIS)

    Qaiser, D.H.; Ghori, G.M.; Sandila, M.P.; Omair, A.

    2013-01-01

    Objective: To determine any significant correlation between the routine haematological parameters of maternal blood and umbilical cord blood of their respective newborns. Study Design: Cross sectional study. Place and Duration of Study: The study was conducted at four public and private hospitals of Karachi including Sindh Government Qatar Hospital, Sindh Government Hospital, Liaquatabad, Ziauddin University Hospital and Chinniot Maternity and Child Hospital, respectively from July 2006 to April 2008. Methodology: Three milliliters venous blood was collected in EDTA containing tube for complete blood count of mothers before delivery. Five milliliters cord blood was collected from the umbilical cord of the babies immediately after delivery by clamping and cutting the babies' end of the cord. For haematological parameters a standard coultergram was done including haemoglobin, RBCs count, haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), white blood cell count, differential leukocyte count and platelet count. Pearson's correlation co-efficient was used to determine an association between the maternal and cord blood parameters. Results: A total of 404 maternal and umbilical cord blood samples were analyzed. All the blood parameters including haemoglobin, RBCs count, HCT, MCV, MCH, white blood cell count, differential leukocyte count and platelet count were found to be high in cord blood as compared to the maternal blood, but they showed a very weak to negligible correlation. Mean Corpuscular Haemoglobin Concentration (MCHC), showed a moderate correlation. Conclusion: Routine haematological parameters of newborns are independent of maternal routine haematological parameters. (author)

  16. Hematological and biochemical characteristics during the transport of dog snapper Lutjanus jocu (Perciformes: Lutjanidae

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    Bruno L. Oliveira

    2018-03-01

    Full Text Available ABSTRACT The objective of this study was to evaluate stress responses in dog snapper (Lutjanus jocu during transport by evaluating their hematological and biochemical responses. Twenty-five wild dog snapper specimens were used in the experiment (220 ± 68 g and 24.5 ± 2.5 cm total length. Blood samples were collected prior to transport (control, and fish were placed in two transport boxes, one with anesthetic and one without anesthetic. Immediately after transport and after 24 h, blood was collected from the fish that underwent each treatment (with anesthetic and without anesthetic. Biochemical and hematological results demonstrated the inefficiency of benzocaine as a stress reliever during handling and transport. Biochemical parameters revealed the effects of stress during transport, and after 24 h, glucose levels and hematological parameters (hemoglobin, erythrocytes, leukocytes, neutrophils and MCH showed a tendency to return to control levels. This study is the first to report stress response measurements of hematological and biochemical indicators in dog snapper, representing an important basis for the planning of future experiments involving the transport and handling of this fish species.

  17. Hematologic and serum chemistry reference intervals for free-ranging lions (Panthera leo).

    Science.gov (United States)

    Maas, Miriam; Keet, Dewald F; Nielen, Mirjam

    2013-08-01

    Hematologic and serum chemistry values are used by veterinarians and wildlife researchers to assess health status and to identify abnormally high or low levels of a particular blood parameter in a target species. For free-ranging lions (Panthera leo) information about these values is scarce. In this study 7 hematologic and 11 serum biochemistry values were evaluated from 485 lions from the Kruger National Park, South Africa. Significant differences between sexes and sub-adult (≤ 36 months) and adult (>36 months) lions were found for most of the blood parameters and separate reference intervals were made for those values. The obtained reference intervals include the means of the various blood parameter values measured in captive lions, except for alkaline phosphatase in the subadult group. These reference intervals can be utilized for free-ranging lions, and may likely also be used as reference intervals for captive lions. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Sensitivity Analysis of Input Parameters for the Dose Assessment from Gaseous Effluents due to the Normal Operation of Jordan Research and Training Reactor

    International Nuclear Information System (INIS)

    Kim, Sukhoon; Lee, Seunghee; Kim, Juyoul; Kim, Juyub; Han, Moonhee

    2015-01-01

    In this study, therefore, the sensitivity analysis of input variables for the dose assessment was performed for reviewing the effect of each parameter on the result after determining the type and range of parameters that could affect the exposure dose of the public. (Since JRTR will be operated by the concept of 'no liquid discharge,' the input parameters used for calculation of dose due to liquid effluents are not considered in the sensitivity analysis.) In this paper, the sensitivity analysis of input parameters for the dose assessment in the vicinity of the site boundary due to gaseous effluents was performed for a total of thirty-five (35) cases. And, detailed results for the input variables that have an significant effect are shown in Figures 1 through 7, respectively. For preparing a R-ER for the operating license of the JRTR, these results will be updated by the additional information and could be applied to predicting the variation trend of the exposure dose in the process of updating the input parameters for the dose assessment reflecting the characteristics of the JRTR site

  19. Hematological, oxidative stress, and immune status profiling in elite combat sport athletes.

    Science.gov (United States)

    Dopsaj, Violeta; Martinovic, Jelena; Dopsaj, Milivoj; Kasum, Goran; Kotur-Stevuljevic, Jelena; Koropanovski, Nenad

    2013-12-01

    The aim of this study was to profile hematological, oxidative stress, and immunological parameters in male athletes who practiced combat sports and to determine whether the type of combat sport influenced the measured parameters. Eighteen karate professionals, 15 wrestlers, and 14 kickboxers participated in the study. Hematological, iron-related, oxidative stress, and immunological parameters were measured at the beginning of a precompetitive period. The general linear model showed significant differences between the karate professionals, wrestlers, and kickboxers with respect to their hematological and iron status parameters (Wilks' Lambda = 0.270, F = 2.186, p stress status (Wilks' Lambda = 0.529, F = 1.940, p < 0.05). The immature reticulocyte fraction was significantly higher in wrestlers (0.30 ± 0.03) compared with kickboxers (0.24 ± 0.04; p < 0.05) and karate professionals (0.26 ± 0.04; p < 0.05). Low hemoglobin density was significantly lower in wrestlers and kickboxers (p < 0.05) compared with karate professionals (karate: 3.51 ± 1.19, wrestlers: 1.95 ± 1.10, and kickboxers: 1.77 ± 0.76). Significant differences were observed between the karate professionals and wrestlers with respect to their pro-oxidant-antioxidant balance (437 ± 103 vs. 323 ± 148, p < 0.05) and superoxide-dismutase activity (SOD) (73 ± 37 vs. 103 ± 30, p < 0.05). All the measured parameters (with the exception of SOD activity) fell within their physiological ranges, indicating that the study participants represented a young and healthy male population. Hematological parameters differed between kickboxers and karate professionals. The low pro-oxidant-antioxidant balance and high SOD activity in wrestlers could be associated with the long-term impact of wrestling as a type of strenuous exercise.

  20. Chromosome dosimetry: its valorization by hematological data

    International Nuclear Information System (INIS)

    Le Go, R.; Doloy, M.T.; Malarbet, J.L.; Veyrat, M.

    1979-01-01

    The assessment of radio-induced biological damage is based upon a number of biological disciplines. Amoung these, two show a particular sensitivity and reliability: cytogenetics and hematology. Using these separately, the data are not always sufficient, but the confrontation of their results reduces the uncertainties in such estimations. Indeed, the mechanisms involved in the responses of both systems are different, as are their dynamics. A brief summary of these mechanisms will illustrate their differences as well as their complementarity

  1. Transferrin receptors on human reticulocytes: variation in site number in hematologic disorders

    International Nuclear Information System (INIS)

    Shumak, K.H.; Rachkewich, R.A.

    1984-01-01

    Assays of binding of 125iodine-labeled ( 125 I) human transferrin were used to study transferrin receptor sites on reticulocytes from 15 normal subjects and from 66 patients with various hematologic disorders. In normal subjects, few or no transferrin receptors were detected whereas the average number of receptors per reticulocyte varied greatly from patient to patient, ranging from 0 to 67,700 in samples, from 35 patients, on which Scatchard analysis of binding of [ 125 I]-transferrin was done. Marked heterogeneity in the number of reticulocyte transferrin receptors in different hematologic disorders was also found in assays with [ 125 I]-OKT9 (monoclonal antibody to the human transferrin receptor). The number of receptors was not correlated with either the reticulocyte count or the hemoglobin

  2. Cytogenetic and hematological studies in the workers occupationally exposed to low levels of ionizing radiation

    International Nuclear Information System (INIS)

    Zakeri, F.; Honarjoo, M.; Rajab pour, M.; Zahadat, A.; Ahmad pour, M.J.; Asghari, K.

    2007-01-01

    Complete text of publication follows. Objective: This study was aimed at detecting both the incidence of chromosomal aberrations and changes in the hematological parameters as biomarkers of possible radiation injury among workers occupationally exposed to low levels of ionizing radiation and detecting the dose-effect relationship. Methods: Samples of peripheral blood were collected from 38 male industrial radiographers exposed to ionizing radiation for 1-16 years and from 24 age- and sex-matched healthy blood donors without radiation history served as control group. All radiation workers were routinely monitored with film badge. Cytogenetic analysis in peripheral blood lymphocytes assessed by the conventional chromosome aberration assay and at least 200 metaphases for each person were scored. The collected blood samples were analyzed for hematological assay using an automatic analyzer Sysmex KX-21, where 14 different parameters were computerized. Mann-Whitney U test was used to compare the frequencies of the unstable aberrations and hematological parameters between test and control groups. Dose-effect relationship and the influence of age and duration of employment was tested by regression analysis. Results and conclusion: The mean frequencies of dicentric and acentric chromosome aberrations were significantly higher in the exposed group than in the control group (P< 0.0005). No correlation between chromosomal aberrations and physical dose and age was observed in the exposed group. Also there is no clear relation between chromosome damage and duration of exposure. However, the increase in chromosome aberrations in the exposed group was not followed by a corresponding hematological depression. The average values of hematological indices were within the reference levels and did not show any significant differences with control group. A tendency of decreasing the absolute lymphocyte count within the referential levels was the only hematological effect in radiation

  3. Efeito do sexo e da idade sobre os parâmetros fisiológicos e hematológicos de bovinos da raça sindi no semi-árido Effect of sex and age on physiologics and hematologics parameters of beef cattle Sindhi from semi-arid tropics

    Directory of Open Access Journals (Sweden)

    Rosangela Maria Nunes da Silva

    2005-02-01

    of october to december 2001. The parameter’s studied were: rectal temperature (RT, breathing (BF and heart (HF frequency and hematologic profile. The readings and blood sampling were performed in the morning between 8:30 and 9:30 hours and in the afternoon between 14:30 and 15:30 once a week. In the experimental period, maximum and minimum temperatures were 35,74°C and 23°C, respectively, and average relative humidity was 51,7%. In the blood samples, which contained EDTA as an anticoagulant, the following tests were done: global count of the number of erythrocytes in modified Newbauer chamber utilizing Gower’s liquid, dosage of hemoglobin by the cyanomethahemoglobin method, determination of the globular volume by the microhematocrit method and calculation of absolute hematimetric indices (average globular volume - AGV, average globular hemoglobin - AGH, concentration of average globular hemoglobin - CAGH. No significant effect (P>0,05 of sex or age on the parameters studied was found. It was conclude that beef cattle of the Sindhi breed, males or females, aged between one and two years shown the same physiological behavior under adverse conditions of the Semi-arid region.

  4. Parâmetros hematológicos e bioquímicos da tilápia-do-Nilo (Oreochromis niloticus L. sob estresse por exposição ao ar Hematological parameters of Nile Tilapia (Oreochromis niloticus L. under air exposure stress

    Directory of Open Access Journals (Sweden)

    Roberta Dias da Silva

    2012-12-01

    Full Text Available No presente trabalho avaliaram-se os parâmetros hematológicos e bioquímicos de exemplares adultos de tilápias (Oreochromis niloticus sob a influência do fator estresse fisiológico em animais submetidos à exposição ao ar durante a engorda em sistema raceway. Foram analisados o eritrograma, teor de hemoglobina, volume globular, o volume corpuscular médio (VCM, a hemoglobina corpuscular média (HCM, a concentração de hemoglobina corpuscular média (CHCM, o leucograma, contagem diferencial de leucócitos, o plaquetograma, a glicose, a proteína total, o colesterol, o triglicerídeo e os eletrólitos (cálcio, cloretos, sódio e potássio. Os resultados revelaram que houve uma homogeneidade de distribuição para hemácias, volume globular, hemoglobina, índices hemantimétricos, proteína total, glicose, colesterol, e íons séricos, indicados pelos valores relativamente baixos do coeficiente de variação. Houve correlação positiva somente para leucócitos totais, células de defesa orgânica (neutrófilos e linfócitos, glicose, colesterol, sódio e cálcio. Quanto ao leucograma, à medida que os animais foram expostos ao ar, o número de leucócitos diminuiu gradativamente (leucopenia, ocorrendo simultaneamente neutrofilia e linfopenia. O índice glicêmico constituiu um bom indicador de estresse fisiológico, devido à hiperglicemia (82,0±20,88mg/dL demonstrada nos tratamentos. A exposição ao ar constituiu um fator de desequilíbrio na homeostase iônica, e na síntese de colesterol endógeno. Entretanto, o tempo de recuperação não ocasionou a completa reabilitação fisiológica frente ao desafio imposto.The present study evaluated the hematological and biochemical parameters of adult tilapia (Oreochromis niloticus under the influence of the physiological stress factor in animals submitted to air exposure during fattening in raceway system. Blood cell count, hemoglobin, hematocrit, mean corpuscular volume (MCV, mean

  5. Hematologic manifestations of Helicobacter pylori infection

    Science.gov (United States)

    Campuzano-Maya, Germán

    2014-01-01

    Helicobacter pylori (H. pylori) is the most common infection in humans, with a marked disparity between developed and developing countries. Although H. pylori infections are asymptomatic in most infected individuals, they are intimately related to malignant gastric conditions such as gastric cancer and gastric mucosa-associated lymphoid tissue (MALT) lymphoma and to benign diseases such as gastritis and duodenal and gastric peptic ulcers. Since it was learned that bacteria could colonize the gastric mucosa, there have been reports in the medical literature of over 50 extragastric manifestations involving a variety medical areas of specialization. These areas include cardiology, dermatology, endocrinology, gynecology and obstetrics, hematology, pneumology, odontology, ophthalmology, otorhinolaryngology and pediatrics, and they encompass conditions with a range of clear evidence between the H. pylori infection and development of the disease. This literature review covers extragastric manifestations of H. pylori infection in the hematology field. It focuses on conditions that are included in international consensus and management guides for H. pylori infection, specifically iron deficiency, vitamin B12 (cobalamin) deficiency, immune thrombocytopenia, and MALT lymphoma. In addition, there is discussion of other conditions that are not included in international consensus and management guides on H. pylori, including auto-immune neutropenia, antiphospholipid syndrome, plasma cell dyscrasias, and other hematologic diseases. PMID:25278680

  6. Role of Extracellular Vesicles in Hematological Malignancies

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    Stefania Raimondo

    2015-01-01

    Full Text Available In recent years the role of tumor microenvironment in the progression of hematological malignancies has been widely recognized. Recent studies have focused on how cancer cells communicate within the microenvironment. Among several factors (cytokines, growth factors, and ECM molecules, a key role has been attributed to extracellular vesicles (EV, released from different cell types. EV (microvesicles and exosomes may affect stroma remodeling, host cell functions, and tumor angiogenesis by inducing gene expression modulation in target cells, thus promoting cancer progression and metastasis. Microvesicles and exosomes can be recovered from the blood and other body fluids of cancer patients and contain and deliver genetic and proteomic contents that reflect the cell of origin, thus constituting a source of new predictive biomarkers involved in cancer development and serving as possible targets for therapies. Moreover, due to their specific cell-tropism and bioavailability, EV can be considered natural vehicles suitable for drug delivery. Here we will discuss the recent advances in the field of EV as actors in hematological cancer progression, pointing out the role of these vesicles in the tumor-host interplay and in their use as biomarkers for hematological malignancies.

  7. Undergraduate hematology – a more integrated approach

    Directory of Open Access Journals (Sweden)

    Di Carlofelice MA

    2016-11-01

    Full Text Available Matteo A Di Carlofelice,* Jack FT Cope*Faculty of Medicine, Imperial College London, London, UK*These authors contributed equally to this workAs fellow medical students we read with great interest the article by Mandan et al1 proposing a dedicated hematology rotation. The authors highlight a gap in clinical hematology teaching, particularly compared to “conventional rotations”.1 Their suggestion has merits and we agree with most of the points raised; however, propose instead for us to build on current teaching as opposed to establishing a new rotation.The integration of theoretical and practical learning was concluded to be beneficial to both teacher and student by Wrenn and Wrenn.2 In the original article it is mentioned that “clinical experience allows students to comprehend and apply this knowledge in practice, enabling the all-important integration of their learning”.1 As we agree that hematology overlaps with other specialties and therefore requires a thorough understanding, we value how a clinical attachment would be useful to solidify lecture-based teaching.View the original paper by Mandan et al.

  8. Hematological findings in children with brucellosis.

    Science.gov (United States)

    Aypak, Adalet; Aypak, Cenk; Bayram, Yasemin

    2015-12-01

    Brucellosis produces a variety of non-specific hematological abnormalities. The aim of the present study was to evaluate the hematological findings in childhood brucellosis. Medical records of children with brucellosis admitted to a tertiary hospital in a 1 year period, were analyzed retrospectively. Sixty-nine patients (mean age, 14.5 ± 3.3 years) were diagnosed with brucellosis. The most common hematological finding was thrombocytopenia (n = 11, 15.9%). Thrombocytosis was detected in five patients (7.3%), leukopenia in four (5.8%), anemia in three (4.3%), and bicytopenia in three (4.3%). None of the patients had pancytopenia. Blood culture was positive for Brucella spp. in 41 patients (59.4%). Among those patients with positive blood culture, six (14.6%) had serum agglutination test titer ≤1/80. Platelet (PLT) count was significantly lower in the bacteremia-positive group. The OR (95%CI) of bacteremia for PLT cut-off 200,000/mm(3) was 0.148 (95%CI: 0.031-0.718) and relative risk was 1.718 (95%CI: 1.244-2.372; P = 0.010). Brucellosis should be kept in mind in the differential diagnosis of isolated thrombocytopenia in pediatric patients from endemic areas. © 2015 Japan Pediatric Society.

  9. Effect of fire smoke on some biochemical parameters in firefighters of Saudi Arabia

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    Rezq Ameen M

    2008-12-01

    Full Text Available Abstract Background Firefighters who are facing fires, are frequently exposed to hazardous materials including carbon monoxide, hydrogen cyanide, hydrogen chloride, benzene, sulphur dioxide, etc. This study aimed to evaluate some relevant serum biochemical and blood hematological changes in activity involved firefighters in comparison to normal subjects. Subjects and Methods Two groups of male firefighters volunteered to participate in the study. The first included 28 firefighters from Jeddah, while the second included 21 firefighters from Yanbu, with overall age ranged 20–48 years. An additional group of 23 male non-firefighters volunteered from both cities as normal control subjects, of age range 20–43 years. Blood samples were collected from all volunteer subjects and investigated for some relevant serum biochemical and blood hematological changes. Results The results obtained showed that, there were statistically significant differences in liver function, kidney function, serum lipid profile, cortisol, creatine kinase, lactate dehydrogenase, iron and its biologically active derivatives, and blood picture in firefighters as compared with the normal control group. These results indicate that, fire smoke mainly affects serum biochemical and blood hematological parameters. Such results might point out to the need for more health protective and prophylactic measures to avoid such hazardous health effects that might endanger firefighters under their highly drastic working conditions. Conclusion Besides using of personal protective equipments for firefighters to protect them against exposure to toxic materials of fire smoke, it is recommended that, firefighters must be under continuous medical follow up through a standard timetabled medical laboratory investigations to allow for early detection of any serum biochemical or blood hematological changes that might happen during their active service life and to allow for early treatment whenever

  10. Implementing a clinical pharmacy service in hematology.

    Science.gov (United States)

    Farias, Tatiane Fernandes; Aguiar, Karina da Silva; Rotta, Inajara; Belletti, Klezia Morais da Silva; Carlotto, Juliane

    2016-01-01

    To implement a clinical pharmacy service focused on the comprehensive review of antineoplastic drugs used in therapy of hematological diseases. An interventional study was conducted in a Brazilian tertiary teaching hospital in two different periods, with and without a clinical pharmacy service, respectively. This service consisted of an antineoplastic prescription validation (analysis of patients' characteristics, laboratory tests, compliance with the therapeutic protocol and with pharmacotechnical parameters). When problems were detected, the pharmacist intervened with the physician or another health professional responsible for the patient. Inpatients and outpatients with hematological diseases were included. We found an increased detection of drug-related problem by 106.5% after implementing the service. Comparing the two periods, an increase in patients' age (26.7 years versus 17.6 years), a predominance of outpatients (54% versus 38%), and an increase in multiple myeloma (13% versus 4%) and non-Hodgkin lymphoma (16% versus 3%) was noted. The most commonly found problems were related to dose (33% versus 25%) and cycle day (14% versus 30%). With regard to clinical impact, the majority had a significant impact (71% versus 58%), and in one patient from the second period could have been fatal. The main pharmaceutical interventions were dose adjustment (35% versus 25%) and drug withdrawal (33% versus 40%). The pharmacy service contributed to increase the detection and resolution of drug-related problems, and it was an effective method to promote the safe and rational use of antineoplastic drugs. Implementar um serviço farmacêutico clínico centrado na revisão completa dos antineoplásicos utilizados no tratamento de doenças hematológicas. Estudo intervencional conduzido em um hospital universitário terciário brasileiro em dois períodos distintos, com base na ausência e na presença do serviço farmacêutico clínico, respectivamente. O referido servi

  11. Hematological findings among styrene-exposed workers in the reinforced plastics industry.

    Science.gov (United States)

    Stengel, B; Touranchet, A; Boiteau, H L; Harousseau, H; Mandereau, L; Hémon, D

    1990-01-01

    A cross-sectional survey of hematological parameters was carried out among 221 workers exposed to styrene in the reinforced plastics industry and 104 controls. Styrene exposure was assessed in both groups by monitoring urinary excretion of styrene metabolites (i.e. mandelic and phenylglyoxylic acids) in post-shift urinary samples collected over five consecutive days. Blood tests were performed with the same counter for all subjects. Information on factors liable to affect hematological parameters was collected from personal questionnaires completed by the workers. Urinary excretion of styrene metabolites varied from 15 to 3740 mg/g creatinine. Exposed subjects exhibited significantly lower mean values of neutrophils and mean corpuscular hemoglobin concentration (MCHC) than controls, and significantly higher mean values for monocytes and mean corpuscular volume. A dose-effect relationship was found for MCHC which decreased with urinary metabolite concentrations. These results remained statistically significant for all parameters after adjustment for age, sex, tobacco and alcohol consumption, place of residence (rural or urban) and time of blood sampling (morning or afternoon) with the exception of neutrophils whose relationship with exposure was essentially explained by smoking. These results are suggestive of a direct effect of styrene-exposure--in the range considered--on the statistical distribution of some hematological parameters.

  12. Glucosylsphingosine Causes Hematological and Visceral Changes in Mice-Evidence for a Pathophysiological Role in Gaucher Disease.

    Science.gov (United States)

    Lukas, Jan; Cozma, Claudia; Yang, Fan; Kramp, Guido; Meyer, Anja; Neßlauer, Anna-Maria; Eichler, Sabrina; Böttcher, Tobias; Witt, Martin; Bräuer, Anja U; Kropp, Peter; Rolfs, Arndt

    2017-10-20

    Glucosylceramide and glucosylsphingosine are the two major storage products in Gaucher disease (GD), an inherited metabolic disorder caused by a deficiency of the lysosomal enzyme glucocerebrosidase. The build-up of glucosylceramide in the endoplasmic reticulum and prominent accumulation in cell lysosomes of tissue macrophages results in decreased blood cell and platelet counts, and skeletal abnormalities. The pathological role of the deacylated form of glucosylceramide, glucosylsphingosine (lyso-Gb1), a recently identified sensitive and specific biomarker for GD, is not well investigated. We established a long-term infusion model in C57BL/6JRj mice to examine the effect of lyso-Gb1 on representative hallmark parameters of GD. Mice received lyso-Gb1 at a dosage of 10 mg·kg -1 per day as a continuous subcutaneous administration, and were routinely checked for blood lyso-Gb1 levels using liquid chromatography-multiple reaction monitoring mass spectrometry (LC/MRM-MS) measurements at four-weekly intervals throughout treatment. The C57BL/6JRj mice showed a stable increase of lyso-Gb1 up to->500-fold greater than the normal reflecting concentrations seen in moderately to severely affected patients. Furthermore, lyso-Gb1 accumulated in peripheral tissues. The mice developed hematological symptoms such as reduced hemoglobin and hematocrit, increased spleen weights and a slight inflammatory tissue response after eight weeks of treatment. The above findings indicate a measurable visceral and hematological response in treated mice that suggests a role for lyso-Gb1 in the development of peripheral signs of GD.

  13. DINAMIKA PROFIL HEMATOLOGI DAN RASIO NETROFIL:LIMFOSIT MONYET EKOR PANJANG (MACACA FASCICULARIS PADA PENGATURAN MIKROKLIMAT RUANGAN

    Directory of Open Access Journals (Sweden)

    Ridzki M.F. Binol

    2010-08-01

    Full Text Available The aim of this study was to obtain the profile of physiological hematology (erythrocyte, PCV, Hb, MCV, MCH, MCHC, and leukocyte and the profile of ratio between neutrophil and lymphocyte of long-tailed macaque (Macaca fascicularis in different microclimate conditions due to the room’s temperature and humidity setting. The symptom of stress was also observed in this study. The research used 10 male macaques aged of 4 until 5 years old which placed in room temperature and humidity of 29,00±1,95°C and 79,52±1,57% respectively 7 days for adaptation period. Further more, the room temperature and humidity were set into 25,79±1,16°C and 80,19±9,05% during 14 days for treatment period. For the last treatment, macaques were reconditioned in room temperature and humidity of 29,00±1,95°C and 79,52±1,57% during 14 days for post-treatment period. Blood samplings were collected through femoralis vein on last day of adaptation period, continued with treatment and post-treatment periods as the day 1, 4, 7, 14, and 28. The results showed that there were not significant difference by room temperature and humidity setting to hematology and neutrophil:lymphocyte ratio. It could be proven by all parameters score obtained were still in normal ranges compared to literatures. There was also no stress symptom based on neutrophil:lymphocyte ratio measurement. Condition with room temperature and humidity of 25,79±1,16°C dan 80,19±9,05% regarded as the most suitable condition for long-tailed macaque’s life

  14. Glucosylsphingosine Causes Hematological and Visceral Changes in Mice—Evidence for a Pathophysiological Role in Gaucher Disease

    Directory of Open Access Journals (Sweden)

    Jan Lukas

    2017-10-01

    Full Text Available Glucosylceramide and glucosylsphingosine are the two major storage products in Gaucher disease (GD, an inherited metabolic disorder caused by a deficiency of the lysosomal enzyme glucocerebrosidase. The build-up of glucosylceramide in the endoplasmic reticulum and prominent accumulation in cell lysosomes of tissue macrophages results in decreased blood cell and platelet counts, and skeletal abnormalities. The pathological role of the deacylated form of glucosylceramide, glucosylsphingosine (lyso-Gb1, a recently identified sensitive and specific biomarker for GD, is not well investigated. We established a long-term infusion model in C57BL/6JRj mice to examine the effect of lyso-Gb1 on representative hallmark parameters of GD. Mice received lyso-Gb1 at a dosage of 10 mg·kg−1 per day as a continuous subcutaneous administration, and were routinely checked for blood lyso-Gb1 levels using liquid chromatography-multiple reaction monitoring mass spectrometry (LC/MRM-MS measurements at four-weekly intervals throughout treatment. The C57BL/6JRj mice showed a stable increase of lyso-Gb1 up to->500-fold greater than the normal reflecting concentrations seen in moderately to severely affected patients. Furthermore, lyso-Gb1 accumulated in peripheral tissues. The mice developed hematological symptoms such as reduced hemoglobin and hematocrit, increased spleen weights and a slight inflammatory tissue response after eight weeks of treatment. The above findings indicate a measurable visceral and hematological response in treated mice that suggests a role for lyso-Gb1 in the development of peripheral signs of GD.

  15. Novel interactions of two α-Hb variants with SEA deletion α0-thalassemia: hematological and molecular analyses.

    Science.gov (United States)

    Srivorakun, Hataichanok; Singha, Kritsada; Fucharoen, Goonnapa; Fucharoen, Supan

    2018-04-01

    To report the hematological and molecular features as well as diagnostic aspects of the hitherto un-described interactions of two rare α-globin chain variants with α 0 -thalassemia commonly found among Southeast Asian populations. The study was done on two adult Thai patients (P1 and P2) who had hypochromic microcytic anemia. Hb analysis was carried out using high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Mutations were identified by PCR and related techniques. Hb analysis of P1 using HPLC showed a normal Hb pattern, but CE demonstrated an abnormal peak at zone 7. DNA sequencing identified a CCG-CTG mutation at codon 95 of the α2 globin gene corresponding to the Hb G-Georgia [α95(G2)Pro → Leu(α2)] previously undescribed in the Thai population. In contrast, Hb analysis of P2 demonstrated an abnormal peak not fully separated from Hb A on HPLC, but not on CE. DNA analysis identified the rarely described Hb Nakhon Ratchasima [α63(E12)Ala → Val(α2)] mutation. Routine DNA analysis detected the SEA deletion α 0 -thalassemia in trans to the Hb variants in both cases. Hematological parameters were compared with those of patients with compound heterozygote for other α-globin variants and α 0 -thalassemia previously documented. Identification of the patients confirmed that interaction of these rare Hb variants with α 0 -thalassemia does not lead to the Hb H disease. Differentiation of these two Hb variants from other clinically relevant hemoglobinopathies in a routine setting is, however, necessary. This can be accomplished using a combined Hb-HPLC and CE analysis followed by PCR-RFLP assays.

  16. Growth, body composition and hematology of juvenile pacu (Piaractus mesopotamicus fed increasing levels of ractopamine

    Directory of Open Access Journals (Sweden)

    A.J.A. Bicudo

    2012-10-01

    Full Text Available The effects of four levels of dietary ractopamine (RAC on growth, body composition and hematology of pacu, Piaractus mesopotamicus juveniles (103.6±3.3g were studied. Fish were housed into 12 circular tanks of 1 m3 - 15 fish per tank - and fed for 60 days with practical diets supplemented with 0; 10; 20 or 40mg RAC per kg of diet, in a totally randomized design trial (n=3. Fish fed diets containing up to 40mg RAC/kg diet for 60 days did not have improved growth or body composition parameters. There were no significant differences in hematocrit, hemoglobin and mean corpuscular hemoglobin. Plasma glucose and triglycerides were significantly smaller in fish fed with RAC; however no significant differences between RAC levels were detected. The inclusion of up to 40mg RAC/kg of diet did not improve growth and body composition, but influenced some hematological and biochemical parameters of juvenile pacu.

  17. Normal behaviour of circulatory parameters during exercise. Reference values for heart rate and systemic blood pressure. The ECCIS Project data. Epidemiologia e Clinica della Cardiopatia Ischemica Silente.

    Science.gov (United States)

    Menghini, F; Dally, L; Fazzini, P F; Menotti, A; Prati, P L; Rovelli, F; Antoniucci, D; Seccareccia, F

    1995-08-01

    The study of simultaneous variations in heart rate (HR) and systemic blood pressure is of great interest in ergometric practice complementing the analysis of the ST segment by ECG. This paper examines data proceeding from 500 consecutive, normal, exercise stress tests with the aim of offering reference values on the step-by-step behaviour of HR, systolic and diastolic blood pressure (SBP, DBP) during exercise in a normal population. The sample comes from a large epidemiological study (ECCIS Project) conducted on 4842 healthy, working men, aged 40-59, which proposes to identify, by a 3 stage procedure, subjects with totally asymptomatic coronary artery disease (type I silent ischemia). A further aim of our paper is to examine the influence of some physiological variables (age, height, weight, body mass index, resting HR, SBP and DBP) on the response to effort of HR, SBP and DBP; reciprocal HR/SBP adjustment during exercise; maximal attained workload and recovery time. Due to a preliminary observation that the rate of step-by-step increase in HR and SBP is inversely related to total duration, the population was split into 4 groups according to exercise tolerance (defined by maximal attained workload) to elaborate reference values. Furthermore our data demonstrate that: 1) SBP increases more rapidly with respect to HR for older and heavier subjects; 2) Exercise tolerance is inversely related to age, baseline HR and SBP, and directly related to weight and height; 3) return to baseline conditions, during recovery, is quicker for subjects with better exercise tolerance and lower baseline HR, SBP and weight.

  18. Hematological disorders in 6-hydroxydopamine-induced rat model of Parkinson’s disease

    OpenAIRE

    Lucian Hritcu

    2008-01-01

    Objective: The present work was undertaken in order to investigate the effects of right-unilateral lesion of substantia nigra neurons by means of 6- hydroxydopamine (6-OHDA), a dopaminergic-selective neurotoxin, on hematological parameters in rats. The primary reason for the using of rat model of Parkinson’s disease was the interest regarding the role of the central dopaminergic system in hematopoiesis regulation because some neurological diseases like Parkinson’s disease are well-correlated ...

  19. Hematology of healthy Florida manatees (Trichechus manatus)

    Science.gov (United States)

    Harvey, J.W.; Harr, K.E.; Murphy, D.; Walsh, M.T.; Nolan, E.C.; Bonde, R.K.; Pate, M.G.; Deutsch, C.J.; Edwards, H.H.; Clapp, W.L.

    2009-01-01

    Background: Hematologic analysis is an important tool in evaluating the general health status of free-ranging manatees and in the diagnosis and monitoring of rehabilitating animals. Objectives: The purpose of this study was to evaluate diagnostically important hematologic analytes in healthy manatees (Trichechus manatus) and to assess variations with respect to location (free ranging vs captive), age class (small calves, large calves, subadults, and adults), and gender. Methods: Blood was collected from 55 free-ranging and 63 captive healthy manatees. Most analytes were measured using a CELL-DYN 3500R; automated reticulocytes were measured with an ADVIA 120. Standard manual methods were used for differential leukocyte counts, reticulocyte and Heinz body counts, and plasma protein and fibrinogen concentrations. Results: Rouleaux, slight polychromasia, stomatocytosis, and low numbers of schistocytes and nucleated RBCs (NRBCs) were seen often in stained blood films. Manual reticulocyte counts were higher than automated reticulocyte counts. Heinz bodies were present in erythrocytes of most manatees. Compared with free-ranging manatees, captive animals had slightly lower MCV, MCH, and eosinophil counts and slightly higher heterophil and NRBC counts, and fibrinogen concentration. Total leukocyte, heterophil, and monocyte counts tended to be lower in adults than in younger animals. Small calves tended to have higher reticulocyte counts and NRBC counts than older animals. Conclusions: Hematologic findings were generally similar between captive and free-ranging manatees. Higher manual reticulocyte counts suggest the ADVIA detects only reticulocytes containing large amounts of RNA. Higher reticulocyte and NRBC counts in young calves probably reflect an increased rate of erythropoiesis compared with older animals. ?? 2009 American Society for Veterinary Clinical Pathology.

  20. Prevalence of hematological abnormalities and malnutrition in HIV ...

    African Journals Online (AJOL)

    2013-10-11

    clinical stage 3). Children who were malnourished were 15 (22.4%). Conclusion: Hematological abnormalities and malnutrition occur in HIV positive children. Key words: Haematological, malnutrition, anaemia, children.

  1. Hematologic and biochemical profiles in Standardbred mares during peripartum.

    Science.gov (United States)

    Mariella, Jole; Pirrone, Alessandro; Gentilini, Fabio; Castagnetti, Carolina

    2014-03-01

    The purposes of this study were to determine physiological changes occurring in hematologic and biochemical parameters in mares between the last month of gestation and the first week after parturition. If a significant change was observed with respect to the reference interval of an adult horse, a further aim of the study was to establish different reference intervals. Blood samples were collected from 62 healthy pregnant Standardbred mares. Seventeen nonpregnant and nonlactating mares were used as a control group. In pregnant mares, blood sampling was conducted every three days from 1 month before the expected foaling date (335 days after the last mating), at parturition, and 7 days after foaling. The barren mares in the control group were sampled once. Results from samples collected 20 and 10 days before parturition, at parturition, and 7 days after were considered in the statistical analysis. A parametric method for all the parameters studied was used to establish reference intervals. Results were compared by repeated measures ANOVA. When significant differences were observed in relation to sampling time, a post hoc analysis was performed (Tukey test). The one-way ANOVA test followed by Dunnett's test was performed to evaluate the presence of a significant difference between each sampling time and the control group. Any significant difference in the blood count parameters at different sampling times was observed by repeated measure ANOVA. Hemoglobin (P biliar acids, total protein, albumin-to-globulin ratio, and calcium were significantly different at different sampling times. Moreover, serum concentration of creatine kinase, aspartate aminotransferase, creatinine, blood urea nitrogen, glucose, lactate, total protein, albumin, albumin-to-globulin ratio, calcium, magnesium, sodium, chloride, potassium, and total, direct, and indirect bilirubin was different from that of the control group. Remarkable changes were not observed in alkaline phosphatase, triglyceride

  2. The Radiation Therapy for Spinal Cord Compression in Hematologic Malignancy

    International Nuclear Information System (INIS)

    Kim, In Ah; Choi, Ihl Bohng; Chung, Su Mi

    1994-01-01

    Spinal cord compression, an oncologic emergency, is a rare complication of hematologic malignancy. Our experience was obtained with a series 32 patients following retrospective analysis for assessing the role of radiation therapy and identifying the prognostic factors affecting on treatment outcome. Diagnosis was usually made by means of radiologic study such as myelography or computerized tomography (CT) or magnetic resonance imaging (MRI) and neurologic examination. Five cases were diagnosed by subjective symptom only with high index of suspicion. In 31 cases, the treatment consisted in radiation therapy alone and the remained one patient had laminectomy before radiation therapy because of diagnostic doubts. Total treatment doses ranged from 800 cGy to 4000 cGy with median of 2999 cGy. Initially large fraction size more than 250 cGy were used in 13 patients with rapidly progressed neurologic deficit. The clinical parameters considered in evaluating the response to treatment were backache, motor-sensory performance and sphincter function. Half on all patients showed good response. Partial response and no response were noted in 37.5% and 12.5%, respectively. Our results showed higher response rate than those of other solid tumor series. The degree of neurologic deficit an that time of diagnosis was the most important predictor of treatment outcome. The elapsed time from development of symptoms to start of treatment was significantly affected on the outcome. But histology of primary tumor, total dose and use of initial large fraction size were not significantly affect on the outcome. These results confirmed the value of early diagnosis and treatment especially in radiosensitive hematologic malignancy

  3. Does exposure to very high levels of natural radiation induce hematological alterations in humans?

    International Nuclear Information System (INIS)

    Ghiassi-Nejad, M.

    2003-01-01

    Full text: It has long been known that total body exposure to moderate doses decrease the number of circulating erythrocytes, platelets, granulocytes, and lymphocytes. However, data on hematopoietic effects of exposure to very low doses of ionizing radiation in humans are scarce. Recently it has been reported that hematological parameters have significant positive associations with the radiation dose received by residents lived near a nuclear power plant. Ramsar, a city in northern Iran, has some inhabited areas with the highest levels of natural radiation studied so far. A population of about 2000 is exposed to average annual radiation levels of 10.2 mGy y -1 and the highest recorded external gamma dose rates are about 130 mGy y -1 . In this study, hematological parameters such as counts of leukocytes, lymphocytes, monocytes, granulocytes, red blood cells, hemoglobin, hematocrit, MCV, MCH, MCHC, RDW, PLT, and MPV were measured in the inhabitants. The results of this study indicated that there was no any statistically significant alteration in hematological parameters of the inhabitants of very high background radiation areas of Ramsar compared to those of a neighboring control area

  4. Some hematological parameters and the prognostic values of CD4, CD8 and total lymphocyte counts and CD4/CD8 cell count ratio in healthy HIV sero-negative, healthy HIV sero-positive and AIDS subjects in Port Harcourt, Nigeria

    Directory of Open Access Journals (Sweden)

    Blessing Didia

    2008-12-01

    Full Text Available OBJECTIVE: The present study attempts to determine normal values of CD4, CD8, CD4/CD8 ratio, total WBC and differential counts, hematocrit and total lymphocyte count (TLC in healthy HIV sero-negative and sero-positive subjects, and to assess the prognostic significance of these parameters in these subjects as compared to AIDS subjects.METHODS: A total of 300 subjects (147 M, 153 F aged between 17 and 71 years were recruited into the study. Subjects were separated according to sex and divided into three groups: Group A: healthy HIV sero-negative subjects; Group B: healthy HIV sero-positive newly diagnosed ART-naïve subjects; and Group C: AIDS subjects. CD4 and CD8 counts were determined by flow cytometry; hematocrit was determined using Hawksley micro-capillary tubes; total WBC and differential counts were determined manually with the improved Neubauer counting chamber; and TLC was obtained by multiplying the percentage of lymphocytes by the total WBC count.RESULTS: For male subjects, significant differences were found in CD4 count, CD4/CD8 count ratio, hematocrit, total WBC and TLC, whereas for female subjects, significant differences were found only in CD4 and CD4/CD8 count ratio in the three groups of subjects. In both sexes, however, these parameters were found to be highest in healthy HIV sero-negative subjects and lowest in AIDS subjects, with HIV sero-positive subjects having intermediate values. CONCLUSION: The results confirm previous reports that the CD4 count and CD4/CD8 count ratio are fairly reliable indicators of the progression of HIV infection. In addition, the results also apparently suggest that the prognostic value of CD8 count is limited and that of TLC possibly sex-dependent. The results could be of importance in our environment since previous reports have been relatively scarce.

  5. MICROTOUGH - calculation of characteristic upper shelf fracture toughness values from microstructural parameters for high strength structural steels with normalized or quenched and tempered microstructure

    International Nuclear Information System (INIS)

    Muenstermann, S.; Dahl, W.; Langenberg, P.; Deimel, P.; Sattler, E.

    2004-01-01

    In modern applications, high strength steels are often utilised to increase the load bearing capacity of components. For safe design it is also necessary that these steels have an adequate fracture toughness. The mechanical properties of high strength structural steels are a result of the production process. In consequence, they are strongly related to the microstructure. Therefore, the aim of the research work in the Microtough project is to develop and apply a new method of quantitative correlation between microstructural parameters and characteristic fracture toughness values. This correlation will on the one hand help for the design of new structural steels with high toughness. On the other hand, it shall allow to characterise the fracture toughness of steel without performing expensive fracture mechanics tests. The research work is carried out in the full temperature range from lower to upper shelf. As both RWTH Aachen University and MPA Uni Stuttgart concentrate on ductile fracture behaviour in their research work, the focus of the presentation lies in the upper shelf. (orig.)

  6. Impact of Bone Marrow Radiation Dose on Acute Hematologic Toxicity in Cervical Cancer: Principal Component Analysis on High Dimensional Data

    International Nuclear Information System (INIS)

    Yun Liang; Messer, Karen; Rose, Brent S.; Lewis, John H.; Jiang, Steve B.; Yashar, Catheryn M.; Mundt, Arno J.; Mell, Loren K.

    2010-01-01

    Purpose: To study the effects of increasing pelvic bone marrow (BM) radiation dose on acute hematologic toxicity in patients undergoing chemoradiotherapy, using a novel modeling approach to preserve the local spatial dose information. Methods and Materials: The study included 37 cervical cancer patients treated with concurrent weekly cisplatin and pelvic radiation therapy. The white blood cell count nadir during treatment was used as the indicator for acute hematologic toxicity. Pelvic BM radiation dose distributions were standardized across patients by registering the pelvic BM volumes to a common template, followed by dose remapping using deformable image registration, resulting in a dose array. Principal component (PC) analysis was applied to the dose array, and the significant eigenvectors were identified by linear regression on the PCs. The coefficients for PC regression and significant eigenvectors were represented in three dimensions to identify critical BM subregions where dose accumulation is associated with hematologic toxicity. Results: We identified five PCs associated with acute hematologic toxicity. PC analysis regression modeling explained a high proportion of the variation in acute hematologicity (adjusted R 2 , 0.49). Three-dimensional rendering of a linear combination of the significant eigenvectors revealed patterns consistent with anatomical distributions of hematopoietically active BM. Conclusions: We have developed a novel approach that preserves spatial dose information to model effects of radiation dose on toxicity, which may be useful in optimizing radiation techniques to avoid critical subregions of normal tissues. Further validation of this approach in a large cohort is ongoing.

  7. Direct Extraction of InP/GaAsSb/InP DHBT Equivalent-Circuit Elements From S-Parameters Measured at Cut-Off and Normal Bias Conditions

    DEFF Research Database (Denmark)

    Johansen, Tom Keinicke; Leblanc, Rémy; Poulain, Julien

    2016-01-01

    A unique direct parameter extraction method for the small-signal equivalent-circuit model of InP/GaAsSb/InP double heterojunction bipolar transistors (DHBTs) is presented. $S$-parameters measured at cut-off bias are used, at first, to extract the distribution factor $X_{0}$ for the base-collector......A unique direct parameter extraction method for the small-signal equivalent-circuit model of InP/GaAsSb/InP double heterojunction bipolar transistors (DHBTs) is presented. $S$-parameters measured at cut-off bias are used, at first, to extract the distribution factor $X_{0}$ for the base......-collector capacitance at zero collector current and the collector-to-emitter overlap capacitance $C_{ceo}$ present in InP DHBT devices. Low-frequency $S$-parameters measured at normal bias conditions then allows the extraction of the external access resistances $R_{bx}$, $R_{e}$, and $R_{cx}$ as well as the intrinsic...

  8. Nanotechnology applications in hematological malignancies (Review)

    Science.gov (United States)

    SAMIR, AHMED; ELGAMAL, BASMA M; GABR, HALA; SABAAWY, HATEM E

    2015-01-01

    A major limitation to current cancer therapies is the development of therapy-related side-effects and dose limiting complications. Moreover, a better understanding of the biology of cancer cells and the mechanisms of resistance to therapy is rapidly developing. The translation of advanced knowledge and discoveries achieved at the molecular level must be supported by advanced diagnostic, therapeutic and delivery technologies to translate these discoveries into useful tools that are essential in achieving progress in the war against cancer. Nanotechnology can play an essential role in this aspect providing a transforming technology that can translate the basic and clinical findings into novel diagnostic, therapeutic and preventive tools useful in different types of cancer. Hematological malignancies represent a specific class of cancer, which attracts special attention in the applications of nanotechnology for cancer diagnosis and treatment. The aim of the present review is to elucidate the emerging applications of nanotechnology in cancer management and describe the potentials of nanotechnology in changing the key fundamental aspects of hematological malignancy diagnosis, treatment and follow-up. PMID:26134389

  9. The PIM kinases in hematological cancers.

    Science.gov (United States)

    Alvarado, Yesid; Giles, Francis J; Swords, Ronan T

    2012-02-01

    The PIM genes represent a family of proto-oncogenes that encode three different serine/threonine protein kinases (PIM1, PIM2 and PIM3) with essential roles in the regulation of signal transduction cascades, which promote cell survival, proliferation and drug resistance. PIM kinases are overexpressed in several hematopoietic tumors and support in vitro and in vivo malignant cell growth and survival, through cell cycle regulation and inhibition of apoptosis. PIM kinases do not have an identified regulatory domain, which means that these proteins are constitutively active once transcribed. They appear to be critical downstream effectors of important oncoproteins and, when overexpressed, can mediate drug resistance to available agents, such as rapamycin. Recent crystallography studies reveal that, unlike other kinases, they possess a hinge region, which creates a unique binding pocket for ATP, offering a target for an increasing number of potent small-molecule PIM kinase inhibitors. Preclinical studies in models of various hematologic cancers indicate that these novel agents show promising activity and some of them are currently being evaluated in a clinical setting. In this review, we profile the PIM kinases as targets for therapeutics in hematologic malignancies.

  10. Common variable immune deficiency with mutated TNFSRF13B gene presenting with autoimmune hematologic manifestations

    Directory of Open Access Journals (Sweden)

    Elpis Mantadakis

    2016-10-01

    Full Text Available Patients with common variable immunodeficiency (CVID develop autoimmune hematologic manifestations. We report a 14-year-old boy with Evans syndrome, who presented at the age of 11.5 years with autoimmune hemolysis and was successfully managed with corticosteroids. Initially, the serum immunoglobulins were within the low-normal range for age, but two years after presentation he definitely fulfilled the diagnostic criteria for CVID, despite a negative history for serious infections. DNA sequencing by PCR of the TNFSRF13B gene that encodes the TACI receptor disclosed the heterozygous mutation C104R that is found in approximately 10–15% of patients with CVID. Common variable immunodeficiency should be considered in the differential diagnosis of autoimmune hematologic manifestations, since its timely diagnosis may considerably affect clinical management and patient outcome.

  11. Evaluation of serum trace element, biochemical and hematological data of a healthy elderly group residing in Sao Paulo city, Brazil

    International Nuclear Information System (INIS)

    Saiki, M.; Vasconcellos, M.B.A.; Sumita, N.M.; Jaluul, O.; Jacob-Filho, W.

    2009-01-01

    In this study, blood serum trace elements, biochemical and hematological parameters were obtained to assess the health status of an elderly population residing in Sao Paulo city, SP, Brazil. Results obtained showed that more than 93% of the studied individuals presented most of the serum trace element concentrations and of the hematological and biochemical data within the reference values used in clinical laboratories. However, the percentage of elderly presenting recommended low density lipoprotein (LDL) cholesterol concentrations was low (70%). The study indicated positive correlation between the concentrations of Zn and LDL-cholesterol (p<0.06). (author)

  12. Clinical, hematological and biochemical alterations in olive flounder, Paralichthys olivaceus following experimental infection by Vibrio scophthalmi

    Science.gov (United States)

    Hematological analysis can provide key values for monitoring fish health conditions. There is no information available on hematological changes of olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined for olive flounder inf...

  13. Comparison of select hematology and serum chemistry analtyes between wild-caught and aquarium-housed lake sturgeon (Acipenser fulvescens)

    Science.gov (United States)

    DiVincenti, Louis; Priest, Heather; Walker, Kyle J.; Wyatt, Jeffrey D.; Dittman, Dawn

    2013-01-01

    Hematology and serum chemistry analytes were compared between wild-caught and aquarium-housed lake sturgeon (Acipenser fulvescens) to potentially improve understanding of medical issues in lake sturgeon. Blood samples were taken from 30 lake sturgeon exhibited in 11 institutions in the United States and from 23 experimentally stocked lake sturgeon caught in gill nets in the lower Genesee River in Rochester, New York, USA. For hematology, only segmented neutrophil count was significantly different, with wild-caught fish having a higher number of circulating neutrophils. For clinical chemistry analytes, chloride, uric acid, calcium, phosphate, glucose, aspartate aminotransferase, triglycerides, and creatine kinase were significantly different between the two cohorts. These differences are likely not clinically significant and are attributable to handling stress, variability in environmental parameters, or differences in nutritional status. This is the first report of hematology and serum chemistry values in aquarium-housed lake sturgeon and provides useful reference intervals for clinicians.

  14. GAMBARAN HEMATOLOGI MENCIT (Mus musculus MODEL TOKSISITAS SUBKRONIS

    Directory of Open Access Journals (Sweden)

    Ita Nur Eka Pujiastuti

    2017-06-01

    Full Text Available Garlic commonly is consumed as medicine to prevent or heal illness or to maintain someone's health. Many societies prefer garlic (Allium sativum among other herbal remedies for cholesterol treatment. It consists of several types, and one of them is single bulb garlic used to treat hypertension. There has been, however, no published research reporting the toxicological properties of single bulb garlic. The purpose of this study was to determine subchronic toxic effects of single bulb garlic administered to mice using hematological parameters. The experiment parameters were hemoglobin and hematocrit levels, the number of erythrocytes and leukocytes. Male mice (Mus musculus strain Balb-C were treated with single bulb garlic extract for 28 days with dosage levels of 0% (N , 0.25% (P1 , 0.5% (P2 , 1% (P3 , and 2% (P4 . Single bulb garlic showed no effect on hemoglobin and hematocrit levels but increased the number of erythrocyte and leucocyte. We concluded that single bulb garlic did not cause subchronic toxic effects.

  15. Hematology of Wild Caught Dubois’s Tree Frog Polypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae

    Directory of Open Access Journals (Sweden)

    Madhusmita Das

    2014-01-01

    Full Text Available Blood was analyzed from eighty (forty males and forty females adult individuals of Polypedates teraiensis to establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV, hemoglobin content (Hb, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, mean corpuscular hemoglobin concentration (MCHC, erythrocyte or red blood cell (RBC count, leukocyte or white blood cell (WBC count, differential leukocyte count, and neutrophil to lymphocyte (N/L ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

  16. Biochemical and Hematological Profiles of Common Carp (Cyprinus Carpio under Sublethal Effects of Trivalent Chromium

    Directory of Open Access Journals (Sweden)

    Zeynab Abedi

    2016-07-01

    Full Text Available Background: In natural waters and/or aquaculture facilities, fish are often exposed to chromium waste and demonstrate cumulative deleterious effects. To our knowledge, there are no studies concerning the effects of trivalent Cr on C. carpio hematology. This study presents hematological and some biochemical parameters of common carp, Cyprinus carpio, affected by sublethal concentration of trivalent chromium. Methods: The fish in the experimental aquaria (three replicates each were exposed to a sublethal chromium chloride concentration of 2 mg L−1, which was prepared as stock solution and added depending on the volume of the aquaria to obtain the required concentration. After a period of 28 days, parameters such as hematocrit (Hct, hemoglobin (Hb, lymphocytes (Lym, neutrophils (Neu, total protein (TP, albumin, immunoglobulin M (IgM, glucose, red and white blood cells (RBC and WBC, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, and mean corpuscular hemoglobin concentration (MCHC were examined. Results: Chromium exposure for 28 days significantly (P0.05 between the Cr-exposed fish and the control. Conclusion: Hematological indices of fish, caused by chromium toxicity to C. carpio, can be secondary responses to toxicants, including exposure to low concentrations of heavy metals, which reflect the launch of stress reaction in the affected fish.

  17. Evaluation of health status of horses immunized with snake venom and montanide adjuvants, IMS 3012 (nanoparticle), ISA 206 and ISA 35 (emulsion based) during polyvalent snake antivenom production: hematological and biochemical assessment.

    Science.gov (United States)

    Waghmare, A B; Salvi, N C; Deopurkar, R L; Shenoy, P A; Sonpetkar, J M

    2014-05-01

    Several biochemical and hematological changes in horses are observed during production of snake antivenom. Although conventional adjuvants like Freund's (Complete and Incomplete) are good immunopotentiators, they produce considerable local reactions in animals. Variety of commercial adjuvants, like montanide adjuvants, having high immunopotentiation and showing lesser side effects are available. The prime objective during antivenom production is to strike a balance between safety of immunized horses and efficacy of the product. In our earlier work, efficacy of montanide group of adjuvants in antivenom production has already been established. The aim of the present work was to assess the safety parameters in horses, viz.: biochemical and hematological, during production of snake antivenom. In the present study, 33 new horses were randomly divided into four groups and hyperimmunized using mixture of snake venoms, viz.: Cobra venom, Russell's viper venom, Krait venom and Echis venom along with montanide adjuvants, IMS 3012, ISA 206, ISA 35 and Incomplete Freund's adjuvant as a control adjuvant; through subcutaneous route at intervals of two weeks. During the immunization period, biochemical and hematological parameters were monitored at 0th, 14th, 21st, 30th and 42nd weeks. The mean hemoglobin values dropped slightly during initial immunization but subsequently regained to normal levels. The mean serum total protein values and globulin levels showed an increment in all the four groups, compared to day zero, vice-versa a slight drop was observed in albumin levels. No significant changes were observed in serum creatinine, bilirubin, alkaline phosphatase and blood urea nitrogen values. Finally, we conclude that montanide adjuvants could be a safer alternative to the conventional adjuvants for primary phase of immunization in antivenom production. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Toxic effects of dietary methylmercury on immune function and hematology in American kestrels (Falco sparverius)

    Science.gov (United States)

    Fallacara, Dawn M.; Halbrook, Richard S.; French, John B.

    2011-01-01

    Fifty-nine adult male American kestrels (Falco sparverius) were assigned to one of three diet formulations including 0 (control), 0.6, and 3.9 μg/g (dry wt) methylmercury (MeHg). Kestrels received their diets daily for 13 weeks to assess the effects of dietary MeHg on immunocompetence. Immunotoxic endpoints included assessment of cell-mediated immunity (CMI) using the phytohemagglutinin (PHA) skin-swelling assay and primary and secondary antibody-mediated immune responses (IR) via the sheep red blood cell (SRBC) hemagglutination assay. Select hematology and histology parameters were evaluated to corroborate the results of functional assays and to assess immunosuppression of T and B cell-dependent components in spleen tissue. Kestrels in the 0.6 and 3.9 μg/g MeHg groups exhibited suppression of CMI, including lower PHA stimulation indexes (p = 0.019) and a 42 to 45% depletion of T cell-dependent splenic lymphoid tissue (p = 0.006). Kestrels in the 0.6 μg/g group exhibited suppression of the primary IR to SRBCs (p = 0.014). MeHg did not have a noticeable effect on the secondary IR (p = 0.166). Elevation of absolute heterophil counts (p p p = 0.003) was apparent in the 3.9 μg/g group at week 12. Heterophilia, or the excess of heterophils in peripheral blood above normal ranges, was apparent in seven of 17 (41%) kestrels in the 3.9 μg/g group and was indicative of an acute inflammatory response or physiological stress. This study revealed that adult kestrels were more sensitive to immunotoxic effects of MeHg at environmentally relevant dietary concentrations than they were to reproductive effects as previously reported.

  19. Single inhalation exposure to 90SrCl2 in the Beagle dog: early hematological effects

    International Nuclear Information System (INIS)

    Gillett, N.; Muggenburg, B.A.; Hahn, F.F.; Boecker, B.B.; Rebar, A.H.; McClellan, R.O.

    1985-01-01

    Young adult Beagle dogs were exposed once to aerosols containing 90 SrCl 2 to obtain initial body burdens ranging from 2.5 to 250 uCi 90 Sr/kg body weight and subsequently observed throughout their life span. All of the dogs are now dead. The primary cause of death over the entire length of the study was radiation-induced osteosarcomas. However, six dogs died at less than 30 days after exposure as a result of a radiation-induced bone marrow aplasia. Review of hematological parameters of all dogs showed a similar, consistent, and often dramatic pancytopenia in those animals having a long-term retained burden of greater than 10 uCi 90 Sr/kg. The hematologic changes were similar to those seen in people exposed to high doses of whole body external radiation. 4 references, 1 figure, 1 table

  20. Relating tumor score to hematology in green turtles with fibropapillomatosis in Hawaii

    Science.gov (United States)

    Work, Thierry M.; Balazs, George H.

    1999-01-01

    The relationship between hematologic status and severity of tumor affliction in green turtles (Chelonia mydas) with fibropapillomatosis (FP) was examined. During 1 wk periods in July 1997 and July 1998, we bled 108 free-ranging green turtles from Pala'au (Molokai, Hawaii, USA) where FP is endemic. Blood was analyzed for hematocrit, estimated total solids, total white blood cell (WBC) count and differential WBC count. Each turtle was assigned a subjective tumor score ranging from 0 (no visible external tumors) to 3 (heavily tumored) that indicated the severity of FP. There was a progressive increase in monocytes and a decrease in all other hematologic parameters except heterophils and total numbers of white blood cells as tumor score increased. These data indicate that tumor score can relate to physiologic status of green turtles afflicted with FP, and that tumor score is a useful field monitor of severity of FP in this species.

  1. Hematological Effects of Herbicide Avalon® (Bentazon+Dicamba in Rats

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    Dragica Brkić

    2011-01-01

    Full Text Available Hematological effects of the herbicide Avalon (GAL-57, a mixture of bentazon and dicamba,were tested on rats. Avalon was administered by gavage at three and four dose levels(250, 500, 1000 and 1250 mg/kg weight/day for 28 and 90 days.Hematological parameters, number of erythrocytes, leukocytes and platelets, hemoglobinconcentration, hematocrit and erythrocyte indexes (MCV, MCH and MCHC weremonitored.The results showed that the herbicide Avalon caused decrease in the values of hemoglobin,hematocrit and erythrocyte indexes (both males and females. The changes(mostly correlated with the doses administered and, in most cases, a lower susceptibilityof females than males was observed. The herbicide GAL-57 had no adverse effect on thenumber of leukocytes, erythrocytes and thrombocytes (both sexes, all doses tested.The results showed that the herbicide Avalon causes weak anemia to the animals tested.Reversibility was apparent during the recovery period of 28 days.

  2. [Peripherally inserted central catheters (PICC) in onco-hematology. PICC line in onco-hematology].

    Science.gov (United States)

    Kabsy, Y; Baudin, G; Vinti, H; Novellas, S; Mannone, L; Chevallier, P; Mounier, N

    2010-09-01

    Peripherally inserted central catheters (PICC) have the advantage of limiting the risk of accidents during installation and are easy to remove. Its use in oncology remains debated because of possible infectious complications. We analyzed 52 PICC in patients with hematological tumor from Nice Hospital. An installation failure was noted in 5.8% of cases. After a follow-up of 15 months, the complication rate was 26.9%, mainly mechanical complications: obstruction (13.5%) or accidental removal (9.6%). The organic complications such as infection or thrombophlebitis represented 3.8%. The median duration was 26 days [2-291]. The longest duration was associated with PICC for chemotherapy (median: 58 days). Frequent blood samples (above: 2 week) were associated with lower duration (median: 23 days). In conclusion, PICC represent a simple and effective alternative to intra-venous central devices in onco-hematology. However, physicians have to focus on short-course treatment.

  3. NF-κB in Hematological Malignancies

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    Véronique Imbert

    2017-05-01

    Full Text Available NF-κB (Nuclear Factor Κ-light-chain-enhancer of activated B cells transcription factors are critical regulators of immunity, stress response, apoptosis, and differentiation. Molecular defects promoting the constitutive activation of canonical and non-canonical NF-κB signaling pathways contribute to many diseases, including cancer, diabetes, chronic inflammation, and autoimmunity. In the present review, we focus our attention on the mechanisms of NF-κB deregulation in hematological malignancies. Key positive regulators of NF-κB signaling can act as oncogenes that are often prone to chromosomal translocation, amplifications, or activating mutations. Negative regulators of NF-κB have tumor suppressor functions, and are frequently inactivated either by genomic deletions or point mutations. NF-κB activation in tumoral cells is also driven by the microenvironment or chronic signaling that does not rely on genetic alterations.

  4. NF-κB in Hematological Malignancies.

    Science.gov (United States)

    Imbert, Véronique; Peyron, Jean-François

    2017-05-31

    NF-κB (Nuclear Factor Κ-light-chain-enhancer of activated B cells) transcription factors are critical regulators of immunity, stress response, apoptosis, and differentiation. Molecular defects promoting the constitutive activation of canonical and non-canonical NF-κB signaling pathways contribute to many diseases, including cancer, diabetes, chronic inflammation, and autoimmunity. In the present review, we focus our attention on the mechanisms of NF-κB deregulation in hematological malignancies. Key positive regulators of NF-κB signaling can act as oncogenes that are often prone to chromosomal translocation, amplifications, or activating mutations. Negative regulators of NF-κB have tumor suppressor functions, and are frequently inactivated either by genomic deletions or point mutations. NF-κB activation in tumoral cells is also driven by the microenvironment or chronic signaling that does not rely on genetic alterations.

  5. BMI1: A Biomarker of Hematologic Malignancies

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    Anagh A. Sahasrabuddhe

    2016-01-01

    Full Text Available BMI1 oncogene is a catalytic member of epigenetic repressor polycomb group proteins. It plays a critical role in the regulation of gene expression pattern and consequently several cellular processes during development, including cell cycle progression, senescence, aging, apoptosis, angiogenesis, and importantly self-renewal of adult stem cells of several lineages. Preponderance of evidences indicates that deregulated expression of PcG protein BMI1 is associated with several human malignancies, cancer stem cell maintenance, and propagation. Importantly, overexpression of BMI1 correlates with therapy failure in cancer patients and tumor relapse. This review discusses the diverse mode of BMI1 regulation at transcriptional, posttranscriptional, and posttranslational levels as well as at various critical signaling pathways regulated by BMI1 activity. Furthermore, this review highlights the role of BMI1 as a biomarker and therapeutic target for several subtypes of hematologic malignancies and the importance to target this biomarker for therapeutic applications.

  6. Vorinostat in solid and <