Sample records for nonlinear macromolecules process

  1. Microfluidic device and method for processing of macromolecules

    DEFF Research Database (Denmark)


    to the first set of outlets is guided through the reaction channels, and second inlet and outlet channels for feeding an enzymatic reagent to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels, wherein the second set of inlets and outlets...

  2. Molecular Dynamics Simulation of Macromolecules Using Graphics Processing Unit

    CERN Document Server

    Xu, Ji; Ge, Wei; Yu, Xiang; Yang, Xiaozhen; Li, Jinghai


    Molecular dynamics (MD) simulation is a powerful computational tool to study the behavior of macromolecular systems. But many simulations of this field are limited in spatial or temporal scale by the available computational resource. In recent years, graphics processing unit (GPU) provides unprecedented computational power for scientific applications. Many MD algorithms suit with the multithread nature of GPU. In this paper, MD algorithms for macromolecular systems that run entirely on GPU are presented. Compared to the MD simulation with free software GROMACS on a single CPU core, our codes achieve about 10 times speed-up on a single GPU. For validation, we have performed MD simulations of polymer crystallization on GPU, and the results observed perfectly agree with computations on CPU. Therefore, our single GPU codes have already provided an inexpensive alternative for macromolecular simulations on traditional CPU clusters and they can also be used as a basis to develop parallel GPU programs to further spee...

  3. Kinetics of quasi-isoenergetic transition processes in biological macromolecules

    CERN Document Server



    A master equation describing the evolution of averaged molecular state occupancies in molecular systems where alternation of molecular energy levels is caused by discrete dichotomous and trichotomous stochastic fields, is derived. This study is focused on the kinetics of quasi-isoenergetic transition processes in the presence of moderately high frequency stochastic field. A novel physical mechanism for temperature-independent transitions in flexible molecular systems is proposed. This mechanism becomes effective when the conformation transitions between quasi-isoenergetic molecular states take place. At room temperatures, stochastic broadening of molecular energy levels predominates the energy of low frequency vibrations accompanying the transition. This leads to a cancellation of the temperature dependence in the stochastically averaged rate constants. As examples, physical interpretations of the temperature-independent onset of P2X$_3$ receptor desensitization in neuronal membranes, as well as degradation o...

  4. CMOS Nonlinear Signal Processing Circuits



    The chapter describes various nonlinear signal processing CMOS circuits, including a high reliable WTA/LTA, simple MED cell, and low-voltage arbitrary order extractor. We focus the discussion on CMOS analog circuit design with reliable, programmable capability, and low voltage operation. It is a practical problem when the multiple identical cells are required to match and realized within a single chip using a conventional process. Thus, the design of high-reliable circuit is indeed needed. Th...

  5. Electron Microscopy and Image Processing: Essential Tools for Structural Analysis of Macromolecules. (United States)

    Belnap, David M


    Macromolecular electron microscopy typically depicts the structures of macromolecular complexes ranging from ∼200 kDa to hundreds of MDa. The amount of specimen required, a few micrograms, is typically 100 to 1000 times less than needed for X-ray crystallography or nuclear magnetic resonance spectroscopy. Micrographs of frozen-hydrated (cryogenic) specimens portray native structures, but the original images are noisy. Computational averaging reduces noise, and three-dimensional reconstructions are calculated by combining different views of free-standing particles ("single-particle analysis"). Electron crystallography is used to characterize two-dimensional arrays of membrane proteins and very small three-dimensional crystals. Under favorable circumstances, near-atomic resolutions are achieved. For structures at somewhat lower resolution, pseudo-atomic models are obtained by fitting high-resolution components into the density. Time-resolved experiments describe dynamic processes. Electron tomography allows reconstruction of pleiomorphic complexes and subcellular structures and modeling of macromolecules in their cellular context. Significant information is also obtained from metal-coated and dehydrated specimens. Copyright © 2015 John Wiley & Sons, Inc.

  6. Nonlinear filtering for LIDAR signal processing

    Directory of Open Access Journals (Sweden)

    D. G. Lainiotis


    Full Text Available LIDAR (Laser Integrated Radar is an engineering problem of great practical importance in environmental monitoring sciences. Signal processing for LIDAR applications involves highly nonlinear models and consequently nonlinear filtering. Optimal nonlinear filters, however, are practically unrealizable. In this paper, the Lainiotis's multi-model partitioning methodology and the related approximate but effective nonlinear filtering algorithms are reviewed and applied to LIDAR signal processing. Extensive simulation and performance evaluation of the multi-model partitioning approach and its application to LIDAR signal processing shows that the nonlinear partitioning methods are very effective and significantly superior to the nonlinear extended Kalman filter (EKF, which has been the standard nonlinear filter in past engineering applications.

  7. Multiorder nonlinear diffraction in frequency doubling processes

    DEFF Research Database (Denmark)

    Saltiel, Solomon M.; Neshev, Dragomir N.; Krolikowski, Wieslaw


    We analyze experimentally light scattering from 2 nonlinear gratings and observe two types of second-harmonic frequency-scattering processes. The first process is identified as Raman–Nath type nonlinear diffraction that is explained by applying only transverse phase-matching conditions. The angular...... position of this type of diffraction is defined by the ratio of the second-harmonic wavelength and the grating period. In contrast, the second type of nonlinear scattering process is explained by the longitudinal phase matching only, being insensitive to the nonlinear grating...

  8. Digital signal processing for fiber nonlinearities [Invited

    DEFF Research Database (Denmark)

    Cartledge, John C.; Guiomar, Fernando P.; Kschischang, Frank R.


    This paper reviews digital signal processing techniques that compensate, mitigate, and exploit fiber nonlinearities in coherent optical fiber transmission systems......This paper reviews digital signal processing techniques that compensate, mitigate, and exploit fiber nonlinearities in coherent optical fiber transmission systems...

  9. Function of terahertz spectra in monitoring the decomposing process of biological macromolecules and in investigating the causes of photoinhibition. (United States)

    Qu, Yuangang; Zhang, Shuai; Lian, Yuji; Kuang, Tingyun


    Chlorophyll a and β-carotene play an important role in harvesting light energy, which is used to drive photosynthesis in plants. In this study, terahertz (THz) and visible range spectra of chlorophyll a and β-carotene and their changes under light treatment were investigated. The results show that the all THz transmission and absorption spectra of chlorophyll a and β-carotene changed upon light treatment, with the maximum changes at 15 min of illumination indicating the greatest changes of the collective vibrational mode of chlorophyll a and β-carotene. The absorption spectra of chlorophyll a in the visible light region decreased upon light treatment, signifying the degradation of chlorophyll a molecules. It can be inferred from these results that the THz spectra are very sensitive in monitoring the changes of the collective vibrational mode, despite the absence of changes in molecular configuration. The THz spectra can therefore be used to monitor the decomposing process of biological macromolecules; however, visible absorption spectra can only be used to monitor the breakdown extent of biological macromolecules.

  10. Input saturation in nonlinear multivariable processes resolved by nonlinear decoupling

    Directory of Open Access Journals (Sweden)

    Jens G. Balchen


    Full Text Available A new method is presented for the resolution of the problem of input saturation in nonlinear multivariable process control by means of elementary nonlinear decoupling (END. Input saturation can have serious consequences particularly in multivariable control because it may lead to very undesirable system behaviour and quite often system instability. Many authors have searched for systematic techniques for designing multivariable control systems in which saturation may occur in any of the control variables (inputs, manipulated variables. No generally accepted method seems to have been presented so far which gives a solution in closed form. The method of elementary nonlinear decoupling (END can be applied directly to the case of saturation control variables by deriving as many control strategies as there are combinations of saturating control variables. The method is demonstrated by the multivariable control of a simulated Fluidized Catalytic Cracker (FCC with very convincing results.

  11. Sensor Network Design for Nonlinear Processes

    Institute of Scientific and Technical Information of China (English)

    李博; 陈丙珍


    This paper presents a method to design a cost-optimal nonredundant sensor network to observe all variables in a general nonlinear process. A mixed integer linear programming model was used to minimize the cost with data classification to check the observability of all unmeasured variables. This work is a starting point for designing sensor networks for general nonlinear processes based on various criteria, such as reliability and accuracy.

  12. Central Limit Theorem for Nonlinear Hawkes Processes

    CERN Document Server

    Zhu, Lingjiong


    Hawkes process is a self-exciting point process with clustering effect whose jump rate depends on its entire past history. It has wide applications in neuroscience, finance and many other fields. Linear Hawkes process has an immigration-birth representation and can be computed more or less explicitly. It has been extensively studied in the past and the limit theorems are well understood. On the contrary, nonlinear Hawkes process lacks the immigration-birth representation and is much harder to analyze. In this paper, we obtain a functional central limit theorem for nonlinear Hawkes process.

  13. Diffusion of macromolecules through sclera. (United States)

    Miao, Heng; Wu, Bi-Dong; Tao, Yong; Li, Xiao-Xin


    To quantify the in vitro permeability coefficient over different topographical locations of porcine sclera to macromolecules with different molecular weight. Fresh equatorial and posterior superotemporal porcine sclera was mounted in a two-chamber diffusion apparatus, and its permeability to fluorescein isothiocyanate (FITC)-conjugated dextrans ranging in molecular weight from 40 kDa to 150 kDa was determined by fluorescence spectrophotometry. The sclera was processed as frozen sections and viewed with a fluorescence microscope. The thickness of the area and the thickness that macromolecules enriched in the surface of sclera were measured. The permeability coefficient (Pc) of porcine sclera to macromolecules was significantly higher (40 kDa, p = 0.028; 70 kDa, p = 0.033; 150 kDa, p = 0.007) in equatorial region than posterior, which could be attributed to the significant difference of thickness (p macromolecules enriched in the scleral surface was thicker for those with larger MW (p macromolecules. Larger macromolecules are more likely to accumulate in scleral surface. The difference between topographical locations may have pharmacokinetic implications when considering transscleral diffusion of macromolecules. © 2012 The Authors. Acta Ophthalmologica © 2012 Acta Ophthalmologica Scandinavica Foundation.

  14. Broadband Nonlinear Signal Processing in Silicon Nanowires

    DEFF Research Database (Denmark)

    Yvind, Kresten; Pu, Minhao; Hvam, Jørn Märcher;

    The fast non-linearity of silicon allows Tbit/s optical signal processing. By choosing suitable dimensions of silicon nanowires their dispersion can be tailored to ensure a high nonlinearity at power levels low enough to avoid significant two-photon abso We have fabricated low insertion and propa......The fast non-linearity of silicon allows Tbit/s optical signal processing. By choosing suitable dimensions of silicon nanowires their dispersion can be tailored to ensure a high nonlinearity at power levels low enough to avoid significant two-photon abso We have fabricated low insertion...... and propagation loss silicon nanowires and use them to demonstrate the broadband capabilities of silicon....

  15. 生物大分子结晶过程的动力学%The Kinetics of Crystallization Process of Biological Macromolecule

    Institute of Scientific and Technical Information of China (English)

    桂林; 李琳; 胡松青; 陈玲; 范治国; 李坚斌


    In this paper ,the crystallization process of biological macromolecules was presented, and the mechanisms and kinetics of crystallization were analyzed.%阐述了生物大分子结晶的一般过程,分析了结晶的机制和动力学.

  16. The maximal process of nonlinear shot noise (United States)

    Eliazar, Iddo; Klafter, Joseph


    In the nonlinear shot noise system-model shots’ statistics are governed by general Poisson processes, and shots’ decay-dynamics are governed by general nonlinear differential equations. In this research we consider a nonlinear shot noise system and explore the process tracking, along time, the system’s maximal shot magnitude. This ‘maximal process’ is a stationary Markov process following a decay-surge evolution; it is highly robust, and it is capable of displaying both a wide spectrum of statistical behaviors and a rich variety of random decay-surge sample-path trajectories. A comprehensive analysis of the maximal process is conducted, including its Markovian structure, its decay-surge structure, and its correlation structure. All results are obtained analytically and in closed-form.

  17. Ultrafast Nonlinear Signal Processing in Silicon Waveguides

    DEFF Research Database (Denmark)

    Oxenløwe, Leif Katsuo; Mulvad, Hans Christian Hansen; Hu, Hao;


    We describe recent demonstrations of exploiting highly nonlinear silicon waveguides for ultrafast optical signal processing. We describe wavelength conversion and serial-to-parallel conversion of 640 Gbit/s data signals and 1.28 Tbit/s demultiplexing and all-optical sampling.......We describe recent demonstrations of exploiting highly nonlinear silicon waveguides for ultrafast optical signal processing. We describe wavelength conversion and serial-to-parallel conversion of 640 Gbit/s data signals and 1.28 Tbit/s demultiplexing and all-optical sampling....

  18. Recent advances in nonlinear speech processing

    CERN Document Server

    Faundez-Zanuy, Marcos; Esposito, Antonietta; Cordasco, Gennaro; Drugman, Thomas; Solé-Casals, Jordi; Morabito, Francesco


    This book presents recent advances in nonlinear speech processing beyond nonlinear techniques. It shows that it exploits heuristic and psychological models of human interaction in order to succeed in the implementations of socially believable VUIs and applications for human health and psychological support. The book takes into account the multifunctional role of speech and what is “outside of the box” (see Björn Schuller’s foreword). To this aim, the book is organized in 6 sections, each collecting a small number of short chapters reporting advances “inside” and “outside” themes related to nonlinear speech research. The themes emphasize theoretical and practical issues for modelling socially believable speech interfaces, ranging from efforts to capture the nature of sound changes in linguistic contexts and the timing nature of speech; labors to identify and detect speech features that help in the diagnosis of psychological and neuronal disease, attempts to improve the effectiveness and performa...

  19. Quantum Information Processing using Nonlinear Optical Effects

    DEFF Research Database (Denmark)

    Andersen, Lasse Mejling

    of the converted idler depends on the other pump. This allows for temporal-mode-multiplexing. When the effects of nonlinear phase modulation (NPM) are included, the phases of the natural input and output modes are changed, reducing the separability. These effects are to some degree mediated by pre......This PhD thesis treats applications of nonlinear optical effects for quantum information processing. The two main applications are four-wave mixing in the form of Bragg scattering (BS) for quantum-state-preserving frequency conversion, and sum-frequency generation (SFG) in second-order nonlinear...... to obtain a 100 % conversion efficiency is to use multiple stages of frequency conversion, but this setup suffers from the combined effects of NPM. This problem is circumvented by using asymmetrically pumped BS, where one pump is continuous wave. For this setup, NPM is found to only lead to linear phase...

  20. Biological Macromolecule Crystallization Database (United States)

    SRD 21 Biological Macromolecule Crystallization Database (Web, free access)   The Biological Macromolecule Crystallization Database and NASA Archive for Protein Crystal Growth Data (BMCD) contains the conditions reported for the crystallization of proteins and nucleic acids used in X-ray structure determinations and archives the results of microgravity macromolecule crystallization studies.

  1. Internal Decoupling in Nonlinear Process Control

    Directory of Open Access Journals (Sweden)

    Jens G. Balchen


    Full Text Available A simple method has been investigated for the total or partial removal of the effect of non-linear process phenomena in multi-variable feedback control systems. The method is based upon computing the control variables which will drive the process at desired rates. It is shown that the effect of model errors in the linearization of the process can be partly removed through the use of large feedback gains. In practice there will be limits on how large gains can he used. The sensitivity to parameter errors is less pronounced and the transient behaviour is superior to that of ordinary PI controllers.

  2. Processing Approach of Non-linear Adjustment Models in the Space of Non-linear Models

    Institute of Scientific and Technical Information of China (English)

    LI Chaokui; ZHU Qing; SONG Chengfang


    This paper investigates the mathematic features of non-linear models and discusses the processing way of non-linear factors which contributes to the non-linearity of a nonlinear model. On the basis of the error definition, this paper puts forward a new adjustment criterion, SGPE.Last, this paper investigates the solution of a non-linear regression model in the non-linear model space and makes the comparison between the estimated values in non-linear model space and those in linear model space.

  3. Nonlinearly perturbed semi-Markov processes

    CERN Document Server

    Silvestrov, Dmitrii


    The book presents new methods of asymptotic analysis for nonlinearly perturbed semi-Markov processes with a finite phase space. These methods are based on special time-space screening procedures for sequential phase space reduction of semi-Markov processes combined with the systematical use of operational calculus for Laurent asymptotic expansions. Effective recurrent algorithms are composed for getting asymptotic expansions, without and with explicit upper bounds for remainders, for power moments of hitting times, stationary and conditional quasi-stationary distributions for nonlinearly perturbed semi-Markov processes. These results are illustrated by asymptotic expansions for birth-death-type semi-Markov processes, which play an important role in various applications. The book will be a useful contribution to the continuing intensive studies in the area. It is an essential reference for theoretical and applied researchers in the field of stochastic processes and their applications that will cont...

  4. Nonlinear Process Fault Diagnosis Based on Serial Principal Component Analysis. (United States)

    Deng, Xiaogang; Tian, Xuemin; Chen, Sheng; Harris, Chris J


    Many industrial processes contain both linear and nonlinear parts, and kernel principal component analysis (KPCA), widely used in nonlinear process monitoring, may not offer the most effective means for dealing with these nonlinear processes. This paper proposes a new hybrid linear-nonlinear statistical modeling approach for nonlinear process monitoring by closely integrating linear principal component analysis (PCA) and nonlinear KPCA using a serial model structure, which we refer to as serial PCA (SPCA). Specifically, PCA is first applied to extract PCs as linear features, and to decompose the data into the PC subspace and residual subspace (RS). Then, KPCA is performed in the RS to extract the nonlinear PCs as nonlinear features. Two monitoring statistics are constructed for fault detection, based on both the linear and nonlinear features extracted by the proposed SPCA. To effectively perform fault identification after a fault is detected, an SPCA similarity factor method is built for fault recognition, which fuses both the linear and nonlinear features. Unlike PCA and KPCA, the proposed method takes into account both linear and nonlinear PCs simultaneously, and therefore, it can better exploit the underlying process's structure to enhance fault diagnosis performance. Two case studies involving a simulated nonlinear process and the benchmark Tennessee Eastman process demonstrate that the proposed SPCA approach is more effective than the existing state-of-the-art approach based on KPCA alone, in terms of nonlinear process fault detection and identification.

  5. Nonlinear Markov Control Processes and Games (United States)


    further research we indicated possible extensions to state spaces with nontrivial geometry, to the controlled nonlinear quantum dynamic semigroups nonlinear Markov semigroup is a one-parameter semigroup of (possibly nonlinear) transformations of the unit simplex in n-dimensional Euclidean...certain mixing property of nonlinear transition probabilities. In case of the semigroup parametrized by continuous time one defines its generator as the

  6. Nonlinear biochemical signal processing via noise propagation. (United States)

    Kim, Kyung Hyuk; Qian, Hong; Sauro, Herbert M


    Single-cell studies often show significant phenotypic variability due to the stochastic nature of intra-cellular biochemical reactions. When the numbers of molecules, e.g., transcription factors and regulatory enzymes, are in low abundance, fluctuations in biochemical activities become significant and such "noise" can propagate through regulatory cascades in terms of biochemical reaction networks. Here we develop an intuitive, yet fully quantitative method for analyzing how noise affects cellular phenotypes based on identifying a system's nonlinearities and noise propagations. We observe that such noise can simultaneously enhance sensitivities in one behavioral region while reducing sensitivities in another. Employing this novel phenomenon we designed three biochemical signal processing modules: (a) A gene regulatory network that acts as a concentration detector with both enhanced amplitude and sensitivity. (b) A non-cooperative positive feedback system, with a graded dose-response in the deterministic case, that serves as a bistable switch due to noise-induced ultra-sensitivity. (c) A noise-induced linear amplifier for gene regulation that requires no feedback. The methods developed in the present work allow one to understand and engineer nonlinear biochemical signal processors based on fluctuation-induced phenotypes.

  7. Recombination Processes and Nonlinear Markov Chains. (United States)

    Pirogov, Sergey; Rybko, Alexander; Kalinina, Anastasia; Gelfand, Mikhail


    Bacteria are known to exchange genetic information by horizontal gene transfer. Since the frequency of homologous recombination depends on the similarity between the recombining segments, several studies examined whether this could lead to the emergence of subspecies. Most of them simulated fixed-size Wright-Fisher populations, in which the genetic drift should be taken into account. Here, we use nonlinear Markov processes to describe a bacterial population evolving under mutation and recombination. We consider a population structure as a probability measure on the space of genomes. This approach implies the infinite population size limit, and thus, the genetic drift is not assumed. We prove that under these conditions, the emergence of subspecies is impossible.

  8. Global satisfactory control for nonlinear integrator processes with long delay

    Institute of Scientific and Technical Information of China (English)

    Yiqun YANG; Guobo XIANG


    Integrator processes with long delay are difficult to control. Nonlinear characteristics of actuators make the control problem more challenging. A technique is proposed in this paper for global satisfactory control (GSC) of such processes with relay-type nonlinearity. An oscillatory control signal is injected into the nonlinear process; the amplitude and frequency of the oscillatory signal are designed to linearise the nonlinear process in the sense of harmonic analysis; and a state feedback controller is configured to implement GSC over the linearised process. An illustrative example is given to demonstrate the effectiveness of the proposed method.

  9. Ion specificities of artificial macromolecules. (United States)

    Liu, Lvdan; Kou, Ran; Liu, Guangming


    Artificial macromolecules are well-defined synthetic polymers, with a relatively simple structure as compared to naturally occurring macromolecules. This review focuses on the ion specificities of artifical macromolecules. Ion specificities are influenced by solvent-mediated indirect ion-macromolecule interactions and also by direct ion-macromolecule interactions. In aqueous solutions, the role of water-mediated indirect ion-macromolecule interactions will be discussed. The addition of organic solvents to aqueous solutions significantly changes the ion specificities due to the formation of water-organic solvent complexes. For direct ion-macromolecule interactions, we will discuss specific ion-pairing interactions for charged macromolecules and specific ion-neutral site interactions for uncharged macromolecules. When the medium conditions change from dilute solutions to crowded environments, the ion specificities can be modified by either the volume exclusion effect, the variation of dielectric constant, or the interactions between ions, macromolecules, and crowding agents.

  10. Shape transformation of lipid vesicles induced by diffusing macromolecules. (United States)

    Góźdź, W T


    The attachment of macromolecules to the surface of a lipid vesicle may cause its deformations such as budding or creation of cylindrical protrusions. Diffusion of the macromolecules in the membranes may cause its shape transformations. The process of shrinking the protrusions due to diffusion of the macromolecules is investigated. It is assumed that macromolecules modify locally the spontaneous curvature and bending rigidity of the lipid membrane. Both spontaneous curvature and bending rigidities depend on the concentration of membrane components. It has been shown that cylindrical protrusions are created when the macromolecules which induce large spontaneous curvature are accumulated at a piece of the vesicle surface. It has been observed that here the elastic constants influence very little the evolution of the vesicle shape caused by diffusing macromolecules and the most important is the value the spontaneous curvature imposed by the macromolecules.

  11. Generalized Mass Action Law and Thermodynamics of Nonlinear Markov Processes

    CERN Document Server

    Gorban, A N


    The nonlinear Markov processes are the measure-valued dynamical systems which preserve positivity. They can be represented as the law of large numbers limits of general Markov models of interacting particles. In physics, the kinetic equations allow Lyapunov functionals (entropy, free energy, etc.). This may be considered as a sort of inheritance of the Lyapunov functionals from the microscopic master equations. We study nonlinear Markov processes that inherit thermodynamic properties from the microscopic linear Markov processes. We develop the thermodynamics of nonlinear Markov processes and analyze the asymptotic assumption, which are sufficient for this inheritance.

  12. Nonlinear spectral unmixing of hyperspectral images using Gaussian processes

    CERN Document Server

    Altmann, Yoann; McLaughlin, Steve; Tourneret, Jean-Yves


    This paper presents an unsupervised algorithm for nonlinear unmixing of hyperspectral images. The proposed model assumes that the pixel reflectances result from a nonlinear function of the abundance vectors associated with the pure spectral components. We assume that the spectral signatures of the pure components and the nonlinear function are unknown. The first step of the proposed method consists of the Bayesian estimation of the abundance vectors for all the image pixels and the nonlinear function relating the abundance vectors to the observations. The endmembers are subsequently estimated using Gaussian process regression. The performance of the unmixing strategy is evaluated with simulations conducted on synthetic and real data.

  13. Analysis of macromolecules, ligands and macromolecule-ligand complexes (United States)

    Von Dreele, Robert B [Los Alamos, NM


    A method for determining atomic level structures of macromolecule-ligand complexes through high-resolution powder diffraction analysis and a method for providing suitable microcrystalline powder for diffraction analysis are provided. In one embodiment, powder diffraction data is collected from samples of polycrystalline macromolecule and macromolecule-ligand complex and the refined structure of the macromolecule is used as an approximate model for a combined Rietveld and stereochemical restraint refinement of the macromolecule-ligand complex. A difference Fourier map is calculated and the ligand position and points of interaction between the atoms of the macromolecule and the atoms of the ligand can be deduced and visualized. A suitable polycrystalline sample of macromolecule-ligand complex can be produced by physically agitating a mixture of lyophilized macromolecule, ligand and a solvent.

  14. Macromolecule-Assisted de novo Protein Folding (United States)

    Choi, Seong Il; Son, Ahyun; Lim, Keo-Heun; Jeong, Hotcherl; Seong, Baik L.


    In the processes of protein synthesis and folding, newly synthesized polypeptides are tightly connected to the macromolecules, such as ribosomes, lipid bilayers, or cotranslationally folded domains in multidomain proteins, representing a hallmark of de novo protein folding environments in vivo. Such linkage effects on the aggregation of endogenous polypeptides have been largely neglected, although all these macromolecules have been known to effectively and robustly solubilize their linked heterologous proteins in fusion or display technology. Thus, their roles in the aggregation of linked endogenous polypeptides need to be elucidated and incorporated into the mechanisms of de novo protein folding in vivo. In the classic hydrophobic interaction-based stabilizing mechanism underlying the molecular chaperone-assisted protein folding, it has been assumed that the macromolecules connected through a simple linkage without hydrophobic interactions and conformational changes would make no effect on the aggregation of their linked polypeptide chains. However, an increasing line of evidence indicates that the intrinsic properties of soluble macromolecules, especially their surface charges and excluded volume, could be important and universal factors for stabilizing their linked polypeptides against aggregation. Taken together, these macromolecules could act as folding helpers by keeping their linked nascent chains in a folding-competent state. The folding assistance provided by these macromolecules in the linkage context would give new insights into de novo protein folding inside the cell. PMID:22949867

  15. A Course on Macromolecules. (United States)

    Horta, Arturo


    Describes a senior-level course that: (1) focuses on the structure and reactions of macromolecules; (2) treats industrial polymers in a unified way; and (3) uses analysis of conformation and conformational statistics as a unifying approach. Also discusses course topics, including polysaccharides, proteins, nucleic acids, and others. (JN)

  16. Bubble nonlinear dynamics and stimulated scattering process (United States)

    Jie, Shi; De-Sen, Yang; Sheng-Guo, Shi; Bo, Hu; Hao-Yang, Zhang; Shi-Yong, Hu


    A complete understanding of the bubble dynamics is deemed necessary in order to achieve their full potential applications in industry and medicine. For this purpose it is first needed to expand our knowledge of a single bubble behavior under different possible conditions including the frequency and pressure variations of the sound field. In addition, stimulated scattering of sound on a bubble is a special effect in sound field, and its characteristics are associated with bubble oscillation mode. A bubble in liquid can be considered as a representative example of nonlinear dynamical system theory with its resonance, and its dynamics characteristics can be described by the Keller-Miksis equation. The nonlinear dynamics of an acoustically excited gas bubble in water is investigated by using theoretical and numerical analysis methods. Our results show its strongly nonlinear behavior with respect to the pressure amplitude and excitation frequency as the control parameters, and give an intuitive insight into stimulated sound scattering on a bubble. It is seen that the stimulated sound scattering is different from common dynamical behaviors, such as bifurcation and chaos, which is the result of the nonlinear resonance of a bubble under the excitation of a high amplitude acoustic sound wave essentially. The numerical analysis results show that the threshold of stimulated sound scattering is smaller than those of bifurcation and chaos in the common condition. Project supported by the Program for Changjiang Scholars and Innovative Research Team in University, China (Grant No. IRT1228) and the Young Scientists Fund of the National Natural Science Foundation of China (Grant Nos. 11204050 and 11204049).

  17. The radiation chemistry of macromolecules

    CERN Document Server


    The Radiation Chemistry of Macromolecules, Volume II is a collection of papers that discusses radiation chemistry of specific systems. Part 1 deals with radiation chemistry of substituted vinyl polymers, particularly polypropylene (PP) as its structure is intermediate between polyethylene and polyisobutylene. This part also discusses polypropylene oxide (PPOx) for it can be prepared in the atactic, isotactic, and optically active forms. One paper focuses on the fundamental chemical processes and the changes in physical properties that give rise to many different applications of polystyrene. An


    Directory of Open Access Journals (Sweden)

    R. G. SILVA


    Full Text Available A new algorithm for model predictive control is presented. The algorithm utilizes a simultaneous solution and optimization strategy to solve the model's differential equations. The equations are discretized by equidistant collocation, and along with the algebraic model equations are included as constraints in a nonlinear programming (NLP problem. This algorithm is compared with the algorithm that uses orthogonal collocation on finite elements. The equidistant collocation algorithm results in simpler equations, providing a decrease in computation time for the control moves. Simulation results are presented and show a satisfactory performance of this algorithm.

  19. Coupled parametric processes in binary nonlinear photonic structures

    CERN Document Server

    Saygin, M Yu


    We study parametric interactions in a new type of nonlinear photonic structures, which is realized in the vicinity of a pair of nonlinear crystals. In this kind of structure, which we call binary, multiple nonlinear optical processes can be implemented simultaneously, owing to multiple phase-matching conditions, fulfilled separately in the constituent crystals. The coupling between the nonlinear processes by means of modes sharing similar frequency is attained by the spatially-broadband nature of the parametric fields. We investigate the spatial properties of the fields generated in the binary structure constructed from periodically poled crystals for the two examples: 1) single parametric down-conversion, and 2) coupled parametric down-conversion and up-conversion processes. The efficacy of the fields' generation in these examples is analyzed through comparison with the cases of traditional single periodically poled crystal and aperiodic photonic structure, respectively. It has been shown that the relative s...

  20. NONLINEAR OPTICS: Nonlinear optical processes in planar waveguides and excitation of surface polaritons (United States)

    Yashkir, O. V.; Yashkir, Yu N.


    An investigation is made of nonlinear optical interaction of light propagating in a planar waveguide with surface polaritons. Reduced wave equations for the amplitudes of the waveguide modes and surface polaritons are used to study the characteristics of generation of surface polaritons of difference frequency, parametric frequency up-conversion of the polaritons, and stimulated Raman scattering by the polaritons. An analysis is made of the characteristic properties of the investigated nonlinear optical processes.

  1. Si-rich Silicon Nitride for Nonlinear Signal Processing Applications. (United States)

    Lacava, Cosimo; Stankovic, Stevan; Khokhar, Ali Z; Bucio, T Dominguez; Gardes, F Y; Reed, Graham T; Richardson, David J; Petropoulos, Periklis


    Nonlinear silicon photonic devices have attracted considerable attention thanks to their ability to show large third-order nonlinear effects at moderate power levels allowing for all-optical signal processing functionalities in miniaturized components. Although significant efforts have been made and many nonlinear optical functions have already been demonstrated in this platform, the performance of nonlinear silicon photonic devices remains fundamentally limited at the telecom wavelength region due to the two photon absorption (TPA) and related effects. In this work, we propose an alternative CMOS-compatible platform, based on silicon-rich silicon nitride that can overcome this limitation. By carefully selecting the material deposition parameters, we show that both of the device linear and nonlinear properties can be tuned in order to exhibit the desired behaviour at the selected wavelength region. A rigorous and systematic fabrication and characterization campaign of different material compositions is presented, enabling us to demonstrate TPA-free CMOS-compatible waveguides with low linear loss (~1.5 dB/cm) and enhanced Kerr nonlinear response (Re{γ} = 16 Wm(-1)). Thanks to these properties, our nonlinear waveguides are able to produce a π nonlinear phase shift, paving the way for the development of practical devices for future optical communication applications.

  2. Construction with macromolecules

    Directory of Open Access Journals (Sweden)

    Stefan Hecht


    Full Text Available The generation of nanosized features via the bottom-up organization of individual atoms and molecules represents one of the most rapidly emerging fields in modern science and technology. In order to gain general and modular access to nanostructures, a construction kit consisting of molecular building blocks with defined nanoscale dimensions, shape, and functionality is required. While nature has developed a hierarchical strategy to construct sophisticated and complex nanostructures, chemistry is just starting to explore artificial macromolecules as a means to synthesize specific nano-objects. In this review, recent advances in achieving control over conformation within macromolecules and the interconnection of conformationally defined subunits by either covalent bonds or self-assembly are discussed in analogy to natural structure evolution.

  3. Saturation process of nonlinear standing waves

    Institute of Scientific and Technical Information of China (English)

    马大猷; 刘克


    The sound pressure of the nonlinear standing waves is distorted as expected, but also tends to saturate as being found in standing-wave tube experiments with increasing sinusoidal excitation. Saturation conditions were not actually reached, owing to limited excitation power, but the evidence of tendency to saturation is without question. It is the purpose of this investigation to find the law of saturation from the existing experimental data. The results of curve fitting indicate that negative feedback limits the growth of sound pressure with increasing excitation, the growth of the fundamental and the second harmonic by the negative feedback of their sound pressures, and the growth of the third and higher harmonics, however, by their energies (sound pressures squared). The growth functions of all the harmonics are derived, which are confirmed by the experiments. The saturation pressures and their properties are found.

  4. Adaptive control method for nonlinear time-delay processes

    Institute of Scientific and Technical Information of China (English)


    Two complex properties,varying time-delay and block-oriented nonlinearity,are very common in chemical engineering processes and not easy to be controlled by routine control methods.Aimed at these two complex properties,a novel adaptive control algorithm the basis of nonlinear OFS(orthonormal functional series) model is proposed.First,the hybrid model which combines OFS and Volterra series is introduced.Then,a stable state feedback strategy is used to construct a nonlinear adaptive control algorithm that can guarantee the closed-loop stability and can track the set point curve without steady-state errors.Finally,control simulations and experiments on a nonlinear process with varying time-delay are presented.A number of experimental results validate the efficiency and superiority of this algorithm.

  5. Nonlinear fiber applications for ultrafast all-optical signal processing (United States)

    Kravtsov, Konstantin

    In the present dissertation different aspects of all-optical signal processing, enabled by the use of nonlinear fibers, are studied. In particular, we focus on applications of a novel heavily GeO2-doped (HD) nonlinear fiber, that appears to be superior to many other types of nonlinear fibers because of its high nonlinearity and suitability for the use in nonlinear optical loop mirrors (NOLMs). Different functions, such as all-optical switching, thresholding, and wavelength conversion, are demonstrated with the HD fibers in the NOLM configuration. These basic functions are later used for realization of ultrafast time-domain demultiplexers, clock recovery, detectors of short pulses in stealth communications, and primitive elements for analog computations. Another important technology that benefits from the use of nonlinear fiber-based signal processing is optical code-division multiple access (CDMA). It is shown in both theory and experiment that all-optical thresholding is a unique way of improving existing detection methods for optical CDMA. Also, it is the way of implementation of true asynchronous optical spread-spectrum networks, which allows full realization of optical CDMA potential. Some aspects of quantum signal processing and manipulation of quantum states are also studied in this work. It is shown that propagation and collisions of Thirring solitons lead to a substantial squeezing of quantum states, which may find applications for generation of squeezed light.

  6. Characterization of the molar mass distribution of macromolecules in beer for different mashing processes using asymmetric flow field-flow fractionation (AF4) coupled with multiple detectors. (United States)

    Choi, Jaeyeong; Zielke, Claudia; Nilsson, Lars; Lee, Seungho


    The macromolecular composition of beer is largely determined by the brewing and the mashing process. It is known that the physico-chemical properties of proteinaceous and polysaccharide molecules are closely related to the mechanism of foam stability. Three types of "American pale ale" style beer were prepared using different mashing protocols. The foam stability of the beers was assessed using the Derek Rudin standard method. Asymmetric flow field-flow fractionation (AF4) in combination with ultraviolet (UV), multiangle light scattering (MALS) and differential refractive index (dRI) detectors was used to separate the macromolecules present in the beers and the molar mass (M) and molar mass distributions (MD) were determined. Macromolecular components were identified by enzymatic treatments with β-glucanase and proteinase K. The MD of β-glucan ranged from 10(6) to 10(8) g/mol. In addition, correlation between the beer's composition and foam stability was investigated (increased concentration of protein and β-glucan was associated with increased foam stability).

  7. Nonlinear Statistical Process Monitoring and Fault Detection Using Kernel ICA

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xi; YAN Wei-wu; ZHAO Xu; SHAO Hui-he


    A novel nonlinear process monitoring and fault detection method based on kernel independent component analysis (ICA) is proposed. The kernel ICA method is a two-phase algorithm: whitened kernel principal component (KPCA) plus ICA. KPCA spheres data and makes the data structure become as linearly separable as possible by virtue of an implicit nonlinear mapping determined by kernel. ICA seeks the projection directions in the KPCA whitened space, making the distribution of the projected data as non-gaussian as possible. The application to the fluid catalytic cracking unit (FCCU) simulated process indicates that the proposed process monitoring method based on kernel ICA can effectively capture the nonlinear relationship in process variables. Its performance significantly outperforms monitoring method based on ICA or KPCA.

  8. Modeling and stability analysis of the nonlinear reactive sputtering process

    Directory of Open Access Journals (Sweden)

    György Katalin


    Full Text Available The model of the reactive sputtering process has been determined from the dynamic equilibrium of the reactive gas inside the chamber and the dynamic equilibrium of the sputtered metal atoms which form the compound with the reactive gas atoms on the surface of the substrate. The analytically obtained dynamical model is a system of nonlinear differential equations which can result in a histeresis-type input/output nonlinearity. The reactive sputtering process has been simulated by integrating these differential equations. Linearization has been applied for classical analysis of the sputtering process and control system design.

  9. An Agent Interaction Based Method for Nonlinear Process Plan Scheduling

    Institute of Scientific and Technical Information of China (English)

    GAO Qinglu; WU Bo; GUO Guang


    This article puts forward a scheduling method for nonlinear process plan shop floor. Task allocation and load balance are realized by bidding mechanism. Though the agent interaction process, the execution of tasks is determined and the coherence of manufacturing decision is verified. The employment of heuristic index can help to optimize the system performance.

  10. Innovation as a Nonlinear Process and the Scientometric Perspective

    NARCIS (Netherlands)

    Leydesdorff, L.; Rotolo, D.; de Nooy, W.; Archambault, E.; Gingras, Y.; Larivière, V.


    The process of innovation follows non-linear patterns across the domains of science, technology, and the economy. Novel bibliometric mapping techniques can be used to investigate and represent distinctive, but complementary perspectives on the innovation process (e.g., "demand" and "supply") as well

  11. Heuristic modeling of macromolecule release from PLGA microspheres

    Directory of Open Access Journals (Sweden)

    Szlęk J


    Full Text Available Jakub Szlęk,1 Adam Pacławski,1 Raymond Lau,2 Renata Jachowicz,1 Aleksander Mendyk11Department of Pharmaceutical Technology and Biopharmaceutics, Jagiellonian University Medical College, Krakow, Poland; 2School of Chemical and Biomedical Engineering, Nanyang Technological University (NTU, SingaporeAbstract: Dissolution of protein macromolecules from poly(lactic-co-glycolic acid (PLGA particles is a complex process and still not fully understood. As such, there are difficulties in obtaining a predictive model that could be of fundamental significance in design, development, and optimization for medical applications and toxicity evaluation of PLGA-based multiparticulate dosage form. In the present study, two models with comparable goodness of fit were proposed for the prediction of the macromolecule dissolution profile from PLGA micro- and nanoparticles. In both cases, heuristic techniques, such as artificial neural networks (ANNs, feature selection, and genetic programming were employed. Feature selection provided by fscaret package and sensitivity analysis performed by ANNs reduced the original input vector from a total of 300 input variables to 21, 17, 16, and eleven; to achieve a better insight into generalization error, two cut-off points for every method was proposed. The best ANNs model results were obtained by monotone multi-layer perceptron neural network (MON-MLP networks with a root-mean-square error (RMSE of 15.4, and the input vector consisted of eleven inputs. The complicated classical equation derived from a database consisting of 17 inputs was able to yield a better generalization error (RMSE of 14.3. The equation was characterized by four parameters, thus feasible (applicable to standard nonlinear regression techniques. Heuristic modeling led to the ANN model describing macromolecules release profiles from PLGA microspheres with good predictive efficiency. Moreover genetic programming technique resulted in classical equation with

  12. Nonlinear partial least squares with Hellinger distance for nonlinear process monitoring

    KAUST Repository

    Harrou, Fouzi


    This paper proposes an efficient data-based anomaly detection method that can be used for monitoring nonlinear processes. The proposed method merges advantages of nonlinear projection to latent structures (NLPLS) modeling and those of Hellinger distance (HD) metric to identify abnormal changes in highly correlated multivariate data. Specifically, the HD is used to quantify the dissimilarity between current NLPLS-based residual and reference probability distributions. The performances of the developed anomaly detection using NLPLS-based HD technique is illustrated using simulated plug flow reactor data.

  13. Nonlinear Dynamic Characteristics of Combustion Wave in SHS Process

    Institute of Scientific and Technical Information of China (English)


    The characteristic of combustion wave and its change were analyzed by numerical value calculation and computer simulation,based on the combustion dynamical model of SHS process. It is shown that with the change of condition parameters in SHS process various time-space order combustion waves appear.It is concluded from non-liner dynamical mechanism analysis that the strong coupling of two non-linear dynamical processes is the dynamical mechanism causing the time-space order dissipation structures.

  14. Relaxation Processes in Nonlinear Optical Polymer Films

    Directory of Open Access Journals (Sweden)

    S.N. Fedosov


    Full Text Available Dielectric properties of the guest-host polystyrene/DR1 system have been studied by the AC dielectric spectroscopy method at frequencies from 1 Hz to 0,5 MHz and by the thermally stimulated depolarization current (TSDC method from – 160 to 0 °C. The relaxation peaks at infra-low frequencies from 10 – 5to 10–2 Hz were also calculated using the Hamon’s approximation. Three relaxation processes, namely, α, β and δ ones were identified from the TSDC peaks, while the ε''(fdependence showed a non-Debye ρ-peak narrowing with temperature. The activation energy of the α-relaxation appeared to be 2,57 eV, while that of the γ-process was 0,52 eV. Temperature dependence of the relaxation time is agreed with the Williams-Landel-Ferry model. The ε''(fpeaks were fitted to Havriliak-Negami’s expression and the corresponding distribution parameters were obtained.

  15. Ultra-Fast Optical Signal Processing in Nonlinear Silicon Waveguides

    DEFF Research Database (Denmark)

    Oxenløwe, Leif Katsuo; Galili, Michael; Pu, Minhao;


    We describe recent demonstrations of exploiting highly nonlinear silicon nanowires for processing Tbit/s optical data signals. We perform demultiplexing and optical waveform sampling of 1.28 Tbit/s and wavelength conversion of 640 Gbit/s data signals....

  16. Nonlinear Evolutions of Stimulated Raman and Brillouin Scattering Processes in Partially Stripped-Ion Plasmas

    Institute of Scientific and Technical Information of China (English)

    胡业民; 胡希伟


    Numerical analyses for the nonlinear evolutions of stimulated Raman scattering (SRS) and stimulated Brillouin scattering (SBS) processes are given. Various effects of the second- and third-order nonlinear susceptibilities on the SRS and SBS processes are studied. The nonlinear evolutions of SRS and SBS processes are atfected more efficiently than their linear growth rates by the nonlinear susceptibility.

  17. Modeling Autoregressive Processes with Moving-Quantiles-Implied Nonlinearity

    Directory of Open Access Journals (Sweden)

    Isao Ishida


    Full Text Available We introduce and investigate some properties of a class of nonlinear time series models based on the moving sample quantiles in the autoregressive data generating process. We derive a test fit to detect this type of nonlinearity. Using the daily realized volatility data of Standard & Poor’s 500 (S&P 500 and several other indices, we obtained good performance using these models in an out-of-sample forecasting exercise compared with the forecasts obtained based on the usual linear heterogeneous autoregressive and other models of realized volatility.

  18. New CMOS Compatible Platforms for Integrated Nonlinear Optical Signal Processing

    CERN Document Server

    Moss, D J


    Nonlinear photonic chips have succeeded in generating and processing signals all-optically with performance far superior to that possible electronically - particularly with respect to speed. Although silicon-on-insulator has been the leading platform for nonlinear optics, its high two-photon absorption at telecommunications wavelengths poses a fundamental limitation. This paper reviews some of the recent achievements in CMOS-compatible platforms for nonlinear optics, focusing on amorphous silicon and Hydex glass, highlighting their potential future impact as well as the challenges to achieving practical solutions for many key applications. These material systems have opened up many new capabilities such as on-chip optical frequency comb generation and ultrafast optical pulse generation and measurement.

  19. Linear and Nonlinear MHD Wave Processes in Plasmas. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Tataronis, J. A.


    This program treats theoretically low frequency linear and nonlinear wave processes in magnetized plasmas. A primary objective has been to evaluate the effectiveness of MHD waves to heat plasma and drive current in toroidal configurations. The research covers the following topics: (1) the existence and properties of the MHD continua in plasma equilibria without spatial symmetry; (2) low frequency nonresonant current drive and nonlinear Alfven wave effects; and (3) nonlinear electron acceleration by rf and random plasma waves. Results have contributed to the fundamental knowledge base of MHD activity in symmetric and asymmetric toroidal plasmas. Among the accomplishments of this research effort, the following are highlighted: Identification of the MHD continuum mode singularities in toroidal geometry. Derivation of a third order ordinary differential equation that governs nonlinear current drive in the singular layers of the Alfvkn continuum modes in axisymmetric toroidal geometry. Bounded solutions of this ODE implies a net average current parallel to the toroidal equilibrium magnetic field. Discovery of a new unstable continuum of the linearized MHD equation in axially periodic circular plasma cylinders with shear and incompressibility. This continuum, which we named “accumulation continuum” and which is related to ballooning modes, arises as discrete unstable eigenfrequency accumulate on the imaginary frequency axis in the limit of large mode numbers. Development of techniques to control nonlinear electron acceleration through the action of multiple coherent and random plasmas waves. Two important elements of this program aye student participation and student training in plasma theory.

  20. Process and meaning: nonlinear dynamics and psychology in visual art. (United States)

    Zausner, Tobi


    Creating and viewing visual art are both nonlinear experiences. Creating a work of art is an irreversible process involving increasing levels of complexity and unpredictable events. Viewing art is also creative with collective responses forming autopoietic structures that shape cultural history. Artists work largely from the chaos of the unconscious and visual art contains elements of chaos. Works of art by the author are discussed in reference to nonlinear dynamics. "Travelogues" demonstrates continued emerging interpretations and a deterministic chaos. "Advice to the Imperfect" signifies the resolution of paradox in the nonlinear tension of opposites. "Quanah" shows the nonlinear tension of opposites as an ongoing personal evolution. "The Mother of All Things" depicts seemingly separate phenomena arising from undifferentiated chaos. "Memories" refers to emotional fixations as limit cycles. "Compassionate Heart," "Wind on the Lake," and "Le Mal du Pays" are a series of works in fractal format focusing on the archetype of the mother and child. "Sameness, Depth of Mystery" addresses the illusion of hierarchy and the dynamics of symbols. In "Chasadim" the origin of worlds and the regeneration of individuals emerge through chaos. References to chaos in visual art mirror the nonlinear complexity of life.

  1. Controlled doping by self-assembled dendrimer-like macromolecules. (United States)

    Wu, Haigang; Guan, Bin; Sun, Yingri; Zhu, Yiping; Dan, Yaping


    Doping via self-assembled macromolecules might offer a solution for developing single atom electronics by precisely placing individual dopants at arbitrary location to meet the requirement for circuit design. Here we synthesize dendrimer-like polyglycerol macromolecules with each carrying one phosphorus atom in the core. The macromolecules are immobilized by the coupling reagent onto silicon surfaces that are pre-modified with a monolayer of undecylenic acid. Nuclear magnetic resonance (NMR) and X-ray photoelectron spectroscopy (XPS) are employed to characterize the synthesized macromolecules and the modified silicon surfaces, respectively. After rapid thermal annealing, the phosphorus atoms carried by the macromolecules diffuse into the silicon substrate, forming dopants at a concentration of 10(17) cm(-3). Low-temperature Hall effect measurements reveal that the ionization process is rather complicated. Unlike the widely reported simple ionization of phosphorus dopants, nitrogen and carbon are also involved in the electronic activities in the monolayer doped silicon.

  2. Controlled doping by self-assembled dendrimer-like macromolecules (United States)

    Wu, Haigang; Guan, Bin; Sun, Yingri; Zhu, Yiping; Dan, Yaping


    Doping via self-assembled macromolecules might offer a solution for developing single atom electronics by precisely placing individual dopants at arbitrary location to meet the requirement for circuit design. Here we synthesize dendrimer-like polyglycerol macromolecules with each carrying one phosphorus atom in the core. The macromolecules are immobilized by the coupling reagent onto silicon surfaces that are pre-modified with a monolayer of undecylenic acid. Nuclear magnetic resonance (NMR) and X-ray photoelectron spectroscopy (XPS) are employed to characterize the synthesized macromolecules and the modified silicon surfaces, respectively. After rapid thermal annealing, the phosphorus atoms carried by the macromolecules diffuse into the silicon substrate, forming dopants at a concentration of 1017 cm‑3. Low-temperature Hall effect measurements reveal that the ionization process is rather complicated. Unlike the widely reported simple ionization of phosphorus dopants, nitrogen and carbon are also involved in the electronic activities in the monolayer doped silicon.

  3. Nonlinear Processes in Magnetic Nanodots under Perpendicular Pumping: Micromagnetic Simulations

    Directory of Open Access Journals (Sweden)

    D.V. Slobodiainuk


    Full Text Available Processes that take place in permalloy nanodots under external electromagnetic pumping are considered. It is shown that in such system similar to bulk samples Suhl and kinetic instability processes are possible. Using micromagnetic simulations approach key features of mode excitation with an external pumping power increase were revealed. Results of the simulations were compared with published experimental data dedicated to investigation of magnetic nanodotes in nonlinear regime.

  4. A non-linear model of economic production processes (United States)

    Ponzi, A.; Yasutomi, A.; Kaneko, K.


    We present a new two phase model of economic production processes which is a non-linear dynamical version of von Neumann's neoclassical model of production, including a market price-setting phase as well as a production phase. The rate of an economic production process is observed, for the first time, to depend on the minimum of its input supplies. This creates highly non-linear supply and demand dynamics. By numerical simulation, production networks are shown to become unstable when the ratio of different products to total processes increases. This provides some insight into observed stability of competitive capitalist economies in comparison to monopolistic economies. Capitalist economies are also shown to have low unemployment.

  5. Optoelectronic and nonlinear optical processes in low dimensional semiconductors

    Indian Academy of Sciences (India)

    B P Singh


    Spatial confinement of quantum excitations on their characteristic wavelength scale in low dimensional materials offers unique possibilities to engineer the electronic structure and thereby control their physical properties by way of simple manipulation of geometrical parameters. This has led to an overwhelming interest in quasi-zero dimensional semiconductors or quantum dots as tunable materials for multitude of exciting applications in optoelectronic and nonlinear optical devices and quantum information processing. Large nonlinear optical response and high luminescence quantum yield expected in these systems is a consequence of huge enhancement of transition probabilities ensuing from quantum confinement. High quantum efficiency of photoluminescence, however, is not usually realized in the case of bare semiconductor nanoparticles owing to the presence of surface states. In this talk, I will focus on the role of quantum confinement and surface states in ascertaining nonlinear optical and optoelectronic properties of II–VI semiconductor quantum dots and their nanocomposites. I will also discuss the influence of nonlinear optical processes on their optoelectronic characteristics.


    Directory of Open Access Journals (Sweden)

    Hasan YILDIZ


    Full Text Available Deep drawing process is one of the main procedures used in different branches of industry. Finding numerical solutions for determination of the mechanical behaviour of this process will save time and money. In die surfaces, which have complex geometries, it is hard to determine the effects of parameters of sheet metal forming. Some of these parameters are wrinkling, tearing, and determination of the flow of the thin sheet metal in the die and thickness change. However, the most difficult one is determination of material properties during plastic deformation. In this study, the effects of all these parameters are analyzed before producing the dies. The explicit non-linear finite element method is chosen to be used in the analysis. The numerical results obtained for non-linear material and contact models are also compared with the experiments. A good agreement between the numerical and the experimental results is obtained. The results obtained for the models are given in detail.

  7. Nonlinear Silicon Photonic Signal Processing Devices for Future Optical Networks

    Directory of Open Access Journals (Sweden)

    Cosimo Lacava


    Full Text Available In this paper, we present a review on silicon-based nonlinear devices for all optical nonlinear processing of complex telecommunication signals. We discuss some recent developments achieved by our research group, through extensive collaborations with academic partners across Europe, on optical signal processing using silicon-germanium and amorphous silicon based waveguides as well as novel materials such as silicon rich silicon nitride and tantalum pentoxide. We review the performance of four wave mixing wavelength conversion applied on complex signals such as Differential Phase Shift Keying (DPSK, Quadrature Phase Shift Keying (QPSK, 16-Quadrature Amplitude Modulation (QAM and 64-QAM that dramatically enhance the telecom signal spectral efficiency, paving the way to next generation terabit all-optical networks.

  8. Preface "Nonlinear processes in oceanic and atmospheric flows"

    Directory of Open Access Journals (Sweden)

    E. García-Ladona


    Full Text Available Nonlinear phenomena are essential ingredients in many oceanic and atmospheric processes, and successful understanding of them benefits from multidisciplinary collaboration between oceanographers, meteorologists, physicists and mathematicians. The present Special Issue on "Nonlinear Processes in Oceanic and Atmospheric Flows" contains selected contributions from attendants to the workshop which, in the above spirit, was held in Castro Urdiales, Spain, in July 2008. Here we summarize the Special Issue contributions, which include papers on the characterization of ocean transport in the Lagrangian and in the Eulerian frameworks, generation and variability of jets and waves, interactions of fluid flow with plankton dynamics or heavy drops, scaling in meteorological fields, and statistical properties of El Niño Southern Oscillation.

  9. Preface "Nonlinear processes in oceanic and atmospheric flows"

    CERN Document Server

    Mancho, A M; Turiel, A; Hernandez-Garcia, E; Lopez, C; Garcia-Ladona, E; 10.5194/npg-17-283-2010


    Nonlinear phenomena are essential ingredients in many oceanic and atmospheric processes, and successful understanding of them benefits from multidisciplinary collaboration between oceanographers, meteorologists, physicists and mathematicians. The present Special Issue on ``Nonlinear Processes in Oceanic and Atmospheric Flows'' contains selected contributions from attendants to the workshop which, in the above spirit, was held in Castro Urdiales, Spain, in July 2008. Here we summarize the Special Issue contributions, which include papers on the characterization of ocean transport in the Lagrangian and in the Eulerian frameworks, generation and variability of jets and waves, interactions of fluid flow with plankton dynamics or heavy drops, scaling in meteorological fields, and statistical properties of El Ni\\~no Southern Oscillation.

  10. High-speed signal processing using highly nonlinear optical fibres

    DEFF Research Database (Denmark)

    Peucheret, Christophe; Oxenløwe, Leif Katsuo; Mulvad, Hans Christian Hansen


    relying on the phase of the optical field. Topics covered include all-optical switching of 640 Gbit/s and 1.28 Tbit/s serial data, wavelength conversion at 640 Gbit/s, optical amplitude regeneration of differential phase shift keying (DPSK) signals, as well as midspan spectral inversion for differential 8......We review recent progress in all-optical signal processing techniques making use of conventional silica-based highly nonlinear fibres. In particular, we focus on recent demonstrations of ultra-fast processing at 640 Gbit/s and above, as well as on signal processing of novel modulation formats...

  11. A general method to study equilibrium partitioning of macromolecules

    DEFF Research Database (Denmark)

    The distribution of macromolecules between a confined microscopic solution and a macroscopic bulk solution plays an important role in understanding separation processes such as Size Exclusion Chromatography (SEC). In this study, we have developed an efficient computational algorithm for obtaining...

  12. Double resonant processes in $\\chi^{(2)}$ nonlinear periodic media


    Konotop, V. V.; Kuzmiak, V.


    In a one-dimensional periodic nonlinear $\\chi^{(2)}$ medium, by choosing a proper material and geometrical parameters of the structure, it is possible to obtain two matching conditions for simultaneous generation of second and third harmonics. This leads to new diversity of the processes of the resonant three-wave interactions, which are discussed within the framework of slowly varying envelope approach. In particular, we concentrate on the fractional conversion of the frequency $\\omega \\to (...

  13. SAR processing with non-linear FM chirp waveforms.

    Energy Technology Data Exchange (ETDEWEB)

    Doerry, Armin Walter


    Nonlinear FM (NLFM) waveforms offer a radar matched filter output with inherently low range sidelobes. This yields a 1-2 dB advantage in Signal-to-Noise Ratio over the output of a Linear FM (LFM) waveform with equivalent sidelobe filtering. This report presents details of processing NLFM waveforms in both range and Doppler dimensions, with special emphasis on compensating intra-pulse Doppler, often cited as a weakness of NLFM waveforms.

  14. A comparison of nonlinear media for parametric all-optical signal processing

    DEFF Research Database (Denmark)

    Martinez Diaz, Jordi; Bohigas Nadal, Jaume; Vukovic, Dragana;


    We systematically compare nonlinear media for parametric signal processing by determining the minimum pump power that is required for a given conversion efficiency in a degenerate four-wave mixing process, including the effect of nonlinear loss....

  15. Nonlinear Statistical Signal Processing: A Particle Filtering Approach

    Energy Technology Data Exchange (ETDEWEB)

    Candy, J


    A introduction to particle filtering is discussed starting with an overview of Bayesian inference from batch to sequential processors. Once the evolving Bayesian paradigm is established, simulation-based methods using sampling theory and Monte Carlo realizations are discussed. Here the usual limitations of nonlinear approximations and non-gaussian processes prevalent in classical nonlinear processing algorithms (e.g. Kalman filters) are no longer a restriction to perform Bayesian inference. It is shown how the underlying hidden or state variables are easily assimilated into this Bayesian construct. Importance sampling methods are then discussed and shown how they can be extended to sequential solutions implemented using Markovian state-space models as a natural evolution. With this in mind, the idea of a particle filter, which is a discrete representation of a probability distribution, is developed and shown how it can be implemented using sequential importance sampling/resampling methods. Finally, an application is briefly discussed comparing the performance of the particle filter designs with classical nonlinear filter implementations.

  16. Predicting speech intelligibility in conditions with nonlinearly processed noisy speech

    DEFF Research Database (Denmark)

    Jørgensen, Søren; Dau, Torsten


    The speech-based envelope power spectrum model (sEPSM; [1]) was proposed in order to overcome the limitations of the classical speech transmission index (STI) and speech intelligibility index (SII). The sEPSM applies the signal-tonoise ratio in the envelope domain (SNRenv), which was demonstrated...... to successfully predict speech intelligibility in conditions with nonlinearly processed noisy speech, such as processing with spectral subtraction. Moreover, a multiresolution version (mr-sEPSM) was demonstrated to account for speech intelligibility in various conditions with stationary and fluctuating...... from computational auditory scene analysis and further support the hypothesis that the SNRenv is a powerful metric for speech intelligibility prediction....

  17. Behavior of macromolecules in adsorbed layers

    Institute of Scientific and Technical Information of China (English)


    A model for describing the behavior of macromolecules in adsorbed layers is developed by introducing a concept of distribution density of layer thickness U based on stochastic process and probabilistic statistics. The molecular behavior of layers adsorbed on clay particle surfaces is discussed; the random distribution and its statistics of the layer thickness are given by incorporating experimental results with an ionic polyelectrolyte with the molecular weight of 1.08×106 and chain charged density of 0.254.

  18. Bayesian framework for modeling diffusion processes with nonlinear drift based on nonlinear and incomplete observations. (United States)

    Wu, Hao; Noé, Frank


    Diffusion processes are relevant for a variety of phenomena in the natural sciences, including diffusion of cells or biomolecules within cells, diffusion of molecules on a membrane or surface, and diffusion of a molecular conformation within a complex energy landscape. Many experimental tools exist now to track such diffusive motions in single cells or molecules, including high-resolution light microscopy, optical tweezers, fluorescence quenching, and Förster resonance energy transfer (FRET). Experimental observations are most often indirect and incomplete: (1) They do not directly reveal the potential or diffusion constants that govern the diffusion process, (2) they have limited time and space resolution, and (3) the highest-resolution experiments do not track the motion directly but rather probe it stochastically by recording single events, such as photons, whose properties depend on the state of the system under investigation. Here, we propose a general Bayesian framework to model diffusion processes with nonlinear drift based on incomplete observations as generated by various types of experiments. A maximum penalized likelihood estimator is given as well as a Gibbs sampling method that allows to estimate the trajectories that have caused the measurement, the nonlinear drift or potential function and the noise or diffusion matrices, as well as uncertainty estimates of these properties. The approach is illustrated on numerical simulations of FRET experiments where it is shown that trajectories, potentials, and diffusion constants can be efficiently and reliably estimated even in cases with little statistics or nonequilibrium measurement conditions.

  19. Nonlinear processes in the strong wave-plasma interaction (United States)

    Pegoraro, Francesco; Califano, Francesco; Attico, Nicola; Bulanov, Sergei


    Nonlinear interactions in hot laboratory and/or astrophysical plasmas are a very efficient mechanism able to transfer the energy from the large to the small spatial scales of the system. As a result, kinetic processes are excited and play a key role in the plasma dynamics since the typical fluid dissipative length scales (where the nonlinear cascade is stopped) are (much) smaller then the kinetic length scales. Then, the key point is the role of the kinetic effects in the global plasma dynamics, i.e. whether the kinetic effects remains confined to the small scales of the system or whether there is a significant feedback on the large scales. Here we will address this problem by discussing the nonlinear kinetic evolution of the electromagnetic beam plasma instability where phase space vortices, as well as large scale vortex like magnetic structures in the physical space, are generated by wave - particle interactions. The role and influence of kinetic effects on the large scale plasma dynamics will be also discussed by addressing the problem of collisionless magnetic reconection.

  20. Experimental characterization of nonlinear processes of whistler branch waves (United States)

    Tejero, E. M.; Crabtree, C.; Blackwell, D. D.; Amatucci, W. E.; Ganguli, G.; Rudakov, L.


    Experiments in the Space Physics Simulation Chamber at the Naval Research Laboratory isolated and characterized important nonlinear wave-wave and wave-particle interactions that can occur in the Earth's Van Allen radiation belts by launching predominantly electrostatic waves in the intermediate frequency range with wave normal angle greater than 85 ° and measuring the nonlinearly generated electromagnetic scattered waves. The scattered waves have a perpendicular wavelength that is nearly an order of magnitude larger than that of the pump wave. Calculations of scattering efficiency from experimental measurements demonstrate that the scattering efficiency is inversely proportional to the damping rate and trends towards unity as the damping rate approaches zero. Signatures of both wave-wave and wave-particle scatterings are also observed in the triggered emission process in which a launched wave resonant with a counter-propagating electron beam generates a large amplitude chirped whistler wave. The possibility of nonlinear scattering or three wave decay as a saturation mechanism for the triggered emission is suggested. The laboratory experiment has inspired the search for scattering signatures in the in situ data of chorus emission in the radiation belts.

  1. Recent Advances in Graphene-Assisted Nonlinear Optical Signal Processing

    Directory of Open Access Journals (Sweden)

    Jian Wang


    Full Text Available Possessing a variety of remarkable optical, electronic, and mechanical properties, graphene has emerged as an attractive material for a myriad of optoelectronic applications. The wonderful optical properties of graphene afford multiple functions of graphene based polarizers, modulators, transistors, and photodetectors. So far, the main focus has been on graphene based photonics and optoelectronics devices. Due to the linear band structure allowing interband optical transitions at all photon energies, graphene has remarkably large third-order optical susceptibility χ(3, which is only weakly dependent on the wavelength in the near-infrared frequency range. The graphene-assisted four-wave mixing (FWM based wavelength conversions have been experimentally demonstrated. So, we believe that the potential applications of graphene also lie in nonlinear optical signal processing, where the combination of its unique large χ(3 nonlinearities and dispersionless over the wavelength can be fully exploited. In this review article, we give a brief overview of our recent progress in graphene-assisted nonlinear optical device and their applications, including degenerate FWM based wavelength conversion of quadrature phase-shift keying (QPSK signal, phase conjugated wavelength conversion by degenerate FWM and transparent wavelength conversion by nondegenerate FWM, two-input and three-input high-base optical computing, and high-speed gate-tunable terahertz coherent perfect absorption (CPA using a split-ring graphene.

  2. Persistence Length of DNA Macromolecule with Kinks

    CERN Document Server

    Simonov, Kyrylo


    The study of configurational parameters of deformed DNA is a relevant problem in research of such important biological process as double helix compactization in cell. The deformations accompanied with local disruptions of the regular macromolecule structure cause significant bending of the double helix, or kinks. In this paper an approach for Kratky-Porod model to calculate persistence length of DNA macromolecule with kinks is developed. The presented approach considers kinks of arbitrary configuration, including two basic types of kinks, type 1 - sharp kink caused by unstacking a single base pair step, and type 2 - intrinsic-induced kink that involves several base pairs. Within developed approach analytical expressions for persistence length, coil size and gyration radius of kinky double helix were obtained.

  3. An Overview of Biological Macromolecule Crystallization (United States)

    Krauss, Irene Russo; Merlino, Antonello; Vergara, Alessandro; Sica, Filomena


    The elucidation of the three dimensional structure of biological macromolecules has provided an important contribution to our current understanding of many basic mechanisms involved in life processes. This enormous impact largely results from the ability of X-ray crystallography to provide accurate structural details at atomic resolution that are a prerequisite for a deeper insight on the way in which bio-macromolecules interact with each other to build up supramolecular nano-machines capable of performing specialized biological functions. With the advent of high-energy synchrotron sources and the development of sophisticated software to solve X-ray and neutron crystal structures of large molecules, the crystallization step has become even more the bottleneck of a successful structure determination. This review introduces the general aspects of protein crystallization, summarizes conventional and innovative crystallization methods and focuses on the new strategies utilized to improve the success rate of experiments and increase crystal diffraction quality. PMID:23727935

  4. Generation of large fifth-order nonlinear phase shifts by use of lossless χ(2) cascaded nonlinear processes

    Institute of Scientific and Technical Information of China (English)

    XU Guang; QIAN Liejia; WANG Tao; FAN Dianyuan; LI Fuming


    It is shown that the cascaded fifth-order nonlinear phase shifts will increase with energy loss in the cascaded processes. Essentially different from the multi-photon absorption accompanied with inherent material nonlinearities, the loss of fundamental wave in a cascaded process is controllable and suppressible. By introducing difference frequencies generated from the reaction between the fundamental and its second harmonic after the cascaded processes, the fundamental wave can be free of energy loss, while the large cascaded fifth-order nonlinear phase shift is maintained.

  5. Nonlinear Optical Microscopy Signal Processing Strategies in Cancer (United States)

    Adur, Javier; Carvalho, Hernandes F; Cesar, Carlos L; Casco, Víctor H


    This work reviews the most relevant present-day processing methods used to improve the accuracy of multimodal nonlinear images in the detection of epithelial cancer and the supporting stroma. Special emphasis has been placed on methods of non linear optical (NLO) microscopy image processing such as: second harmonic to autofluorescence ageing index of dermis (SAAID), tumor-associated collagen signatures (TACS), fast Fourier transform (FFT) analysis, and gray level co-occurrence matrix (GLCM)-based methods. These strategies are presented as a set of potential valuable diagnostic tools for early cancer detection. It may be proposed that the combination of NLO microscopy and informatics based image analysis approaches described in this review (all carried out on free software) may represent a powerful tool to investigate collagen organization and remodeling of extracellular matrix in carcinogenesis processes. PMID:24737930

  6. A simple nonlinear PD controller for integrating processes. (United States)

    Dey, Chanchal; Mudi, Rajani K; Simhachalam, Dharmana


    Many industrial processes are found to be integrating in nature, for which widely used Ziegler-Nichols tuned PID controllers usually fail to provide satisfactory performance due to excessive overshoot with large settling time. Although, IMC (Internal Model Control) based PID controllers are capable to reduce the overshoot, but little improvement is found in the load disturbance response. Here, we propose an auto-tuning proportional-derivative controller (APD) where a nonlinear gain updating factor α continuously adjusts the proportional and derivative gains to achieve an overall improved performance during set point change as well as load disturbance. The value of α is obtained by a simple relation based on the instantaneous values of normalized error (eN) and change of error (ΔeN) of the controlled variable. Performance of the proposed nonlinear PD controller (APD) is tested and compared with other PD and PID tuning rules for pure integrating plus delay (IPD) and first-order integrating plus delay (FOIPD) processes. Effectiveness of the proposed scheme is verified on a laboratory scale servo position control system.

  7. Macromolecules in Undergraduate Physical Chemistry. (United States)

    Mattice, Wayne L.


    Suggests the topic of macromolecules and synthetic polymers be included in undergraduate courses. Two macromolecular systems (polyethylene in a state unperturbated by long-range interactions and a polypeptide undergoing a helix-coil transition) are described which are suitable for inclusion in the statistical mechanics section of physical…

  8. Ion binding to biological macromolecules. (United States)

    Petukh, Marharyta; Alexov, Emil


    Biological macromolecules carry out their functions in water and in the presence of ions. The ions can bind to the macromolecules either specifically or non-specifically, or can simply to be a part of the water phase providing physiological gradient across various membranes. This review outlines the differences between specific and non-specific ion binding in terms of the function and stability of the corresponding macromolecules. Furthermore, the experimental techniques to identify ion positions and computational methods to predict ion binding are reviewed and their advantages compared. It is indicated that specifically bound ions are relatively easier to be revealed while non-specifically associated ions are difficult to predict. In addition, the binding and the residential time of non-specifically bound ions are very much sensitive to the environmental factors in the cells, specifically to the local pH and ion concentration. Since these characteristics differ among the cellular compartments, the non-specific ion binding must be investigated with respect to the sub-cellular localization of the corresponding macromolecule.

  9. DNA compaction by nonbinding macromolecules

    NARCIS (Netherlands)

    Vries, de R.J.


    Compaction of DNA by nonbinding macromolecules such as uncharged flexible polymer chains and negatively charged globular proteins is thought to have various applications in biophysics, for example in the formation of a nucleoid structure in bacteria. A simple experimental model that has been very

  10. DNA compaction by nonbinding macromolecules

    NARCIS (Netherlands)

    Vries, de R.J.


    Compaction of DNA by nonbinding macromolecules such as uncharged flexible polymer chains and negatively charged globular proteins is thought to have various applications in biophysics, for example in the formation of a nucleoid structure in bacteria. A simple experimental model that has been very we


    Macromolecules in the pore fluid influence the mobility of hydrophobic compounds through soils. his study evaluated the significance of macromolecules in facilitating chemical transport under laboratory conditions. Partition coefficients between 14C-labeled hexachlorobenzene and ...

  12. Nonlinear calibration and data processing of the solar radio burst

    Institute of Scientific and Technical Information of China (English)

    颜毅华; 谭程明; 徐龙; 姬慧荣; 傅其骏; 宋国乡


    The processes of the sudden energy release and energy transfer, and particle accelerations are the most challenge fundamental problems in solar physics as well as in astrophysics. Nowadays, there has been no direct measurement of the plasma parameters and magnetic fields at the coronal energy release site. Under the certain hypothesis of radiation mechanism and transmission process, radio measurement is almost the only method to diagnose coronal magnetic field. The broadband dynamic solar radio spectrometer that has been finished recently in China has higher time and frequency resolutions. Thus it plays an important role during the research of the 23rd solar cycle in China. Sometimes when there were very large bursts, the spectrometer will be overflowed. It needs to take some special process to discriminate the instrument and interference effects from solar burst signals. According to the characteristic of the solar radio broadband dynamic spectrometer, we developed a nonlinear calibration method to deal with the overflow of instrument, and introduced channel-modification method to deal with images. Finally the interference is eliminated with the help of the wavelet method. Here we take the analysis of the well-known solar-terrestrial event on July 14th, 2000 as the example. It shows the feasibility and validity of the method mentioned above. These methods can also be applied to other issues.

  13. Nonlinear closure relations theory for transport processes in nonequilibrium systems. (United States)

    Sonnino, Giorgio


    A decade ago, a macroscopic theory for closure relations has been proposed for systems out of Onsager's region. This theory is referred to as the thermodynamic field theory (TFT). The aim of this work was to determine the nonlinear flux-force relations that respect the thermodynamic theorems for systems far from equilibrium. We propose a formulation of the TFT where one of the basic restrictions, namely, the closed-form solution for the skew-symmetric piece of the transport coefficients, has been removed. In addition, the general covariance principle is replaced by the De Donder-Prigogine thermodynamic covariance principle (TCP). The introduction of TCP requires the application of an appropriate mathematical formalism, which is referred to as the entropy-covariant formalism. By geometrical arguments, we prove the validity of the Glansdorff-Prigogine universal criterion of evolution. A new set of closure equations determining the nonlinear corrections to the linear ("Onsager") transport coefficients is also derived. The geometry of the thermodynamic space is non-Riemannian. However, it tends to be Riemannian for high values of the entropy production. In this limit, we recover the transport equations found by the old theory. Applications of our approach to transport in magnetically confined plasmas, materials submitted to temperature, and electric potential gradients or to unimolecular triangular chemical reactions can be found at references cited herein. Transport processes in tokamak plasmas are of particular interest. In this case, even in the absence of turbulence, the state of the plasma remains close to (but, it is not in) a state of local equilibrium. This prevents the transport relations from being linear.

  14. Nonlinear properties of and nonlinear processing in hydrogenated amorphous silicon waveguides

    DEFF Research Database (Denmark)

    Kuyken, B.; Ji, Hua; Clemmen, S.


    We propose hydrogenated amorphous silicon nanowires as a platform for nonlinear optics in the telecommunication wavelength range. Extraction of the nonlinear parameter of these photonic nanowires reveals a figure of merit larger than 2. It is observed that the nonlinear optical properties...... of these waveguides degrade with time, but that this degradation can be reversed by annealing the samples. A four wave mixing conversion efficiency of + 12 dB is demonstrated in a 320 Gbit/s serial optical waveform data sampling experiment in a 4 mm long photonic nanowire....

  15. Nonlinear quantum electrodynamic and electroweak processes in strong laser fields

    Energy Technology Data Exchange (ETDEWEB)

    Meuren, Sebastian


    Various nonlinear electrodynamic and electroweak processes in strong plane-wave laser fields are considered with an emphasis on short-pulse effects. In particular, the momentum distribution of photoproduced electron-positron pairs is calculated numerically and a semiclassical interpretation of its characteristic features is established. By proving the optical theorem, compact double-integral expressions for the total pair-creation probability are obtained and numerically evaluated. The exponential decay of the photon wave function in a plane wave is included by solving the Schwinger-Dyson equations to leading-order in the quasistatic approximation. In this respect, the polarization operator in a plane wave is investigated and its Ward-Takahashi identity verified. A classical analysis indicates that a photoproduced electron-positron pair recollides for certain initial conditions. The contributions of such recollision processes to the polarization operator are identified and calculated both analytically and numerically. Furthermore, the existence of nontrivial electron-spin dynamics induced by quantum fluctuations is verified for ultra-short laser pulses. Finally, the exchange of weak gauge bosons is considered, which is essential for neutrino-photon interactions. In particular, the axial-vector-vector coupling tensor is calculated and the so-called Adler-Bell-Jackiw (ABJ) anomaly investigated.

  16. All-optical signal processing in quadratic nonlinear materials

    DEFF Research Database (Denmark)

    Johansen, Steffen Kjær


    of materials with a second order nonlinearity, the so-called X(2) materials, is faster and stronger than that of more conventional materials with a cubic nonlinearity. The X(2) materials support spatial solitons consisting of two coupled components, the fundamental wave (FW) and its second harmonic (SH......). During this project the interaction between such spatial solitons has been investigated theoretically through perturbation theory and experimentally via numerical simulations. The outcome of this research isnew theoretical tools for quantitatively predicting the escape angle, i.e. the angle of incidence...... and exploitation of these cubic nonlinearities in two-period QPM wave-guides has been another area of investigation. Introducing the second period might make practical engineering of the nonlinearities possible. A major result is the discovery that cubic nonlinearities leads to an enhancement of the bandwidth...

  17. New Algorithm Model for Processing Generalized Dynamic Nonlinear Data Derived from Deformation Monitoring Network

    Institute of Scientific and Technical Information of China (English)

    LIN Xiangguo; LIANG Yong


    The processing of nonlinear data was one of hot topics in surveying and mapping field in recent years.As a result, many linear methods and nonlinear methods have been developed.But the methods for processing generalized nonlinear surveying and mapping data, especially for different data types and including unknown parameters with random or nonrandom, are seldom noticed.A new algorithm model is presented in this paper for processing nonlinear dynamic multiple-period and multiple-accuracy data derived from deformation monitoring network.

  18. Mapping of brain macromolecules and their use for spectral processing of (1)H-MRSI data with an ultra-short acquisition delay at 7 T. (United States)

    Považan, Michal; Hangel, Gilbert; Strasser, Bernhard; Gruber, Stephan; Chmelik, Marek; Trattnig, Siegfried; Bogner, Wolfgang


    Long echo time (TE) MR spectroscopy (MRS) sequences are sensitive only to metabolites of low molecular weight. At shorter TE, significantly more metabolite signals are detectable, including broad signals of high-molecular-weight macromolecules (MMs). Although the presence of MM resonances can bias metabolite quantification at short TE, proper quantification of MMs is important since MMs themselves may serve as potentially valuable biomarkers for many pathologies. We have therefore developed an FID-based 2D-MR Spectroscopic Imaging (2D-MRSI) sequence to map MMs in healthy brain tissue at 7 T within a scan time of ~17 min and a repetition time of 879 ms. This 2D-MRSI technique provides MM maps over a whole slice (i.e., including cortical gray matter) at an ultra-short acquisition delay of 1.3 ms, using double inversion for efficient nulling of low-molecular-weight metabolites. The optimal sequence parameters were estimated using Bloch simulations, phantom testing, and in vivo validation. The acquired in vivo MM spectra (n=6) included nine distinct MM peaks in the range of ~0.9-3.7 ppm. The measured average MM spectrum was incorporated into the LCModel basis set and utilized for further quantification of MRSI data sets without metabolite nulling, which were acquired in five additional volunteers. The quantification results for two basis sets, one including the MMs and one without MM spectrum, were compared. Due to the high spectral resolution and full signal detection provided by the FID-MRSI sequence, we could successfully map five important brain metabolites. Most quantified metabolite signal amplitudes were significantly lower since the inclusion of MMs into the basis set corrected the overestimation of metabolite signals. The precision of fit (i.e., Cramér Rao lower bounds) remained unchanged. Our MM maps show that the overall MM contribution was higher in gray matter than in white matter. In conclusion, the acquired MM spectrum improved the accuracy of

  19. System Identification and Filtering of Nonlinear Controlled Markov Processes by Canonical Variate Analysis (United States)


    In this Phase I SBIR study, new methods are developed for the system identification and stochastic filtering of nonlinear controlled Markov processes...state space Markov process models and canonical variate analysis (CVA) for obtaining optimal nonlinear procedures for system identification and stochastic

  20. Application of Novel Nonlinear Optical Materials to Optical Processing (United States)

    Banerjee, Partha P.


    We describe wave mixing and interactions in nonlinear photorefractive polymers and disodium flourescein. Higher diffracted orders yielding forward phase conjugation can be generated in a two-wave mixing geometry in photorefractive polymers, and this higher order can be used for image edge enhancement and correlation. Four-wave mixing and phase conjugation is studied using nonlinear disodium floureschein, and the nature and properties of gratings written in this material are investigated.

  1. Flexibility and Stability of Metal Coordination Macromolecules. (United States)

    Jiang, Heyan; Geng, Diya; Liu, Dapeng; Lanigan, Nicholas; Wang, Xiaosong


    The effect of chain structure on flexibility and stability of macromolecules containing weak P-Fe metal coordination bonds is studied. Migration insertion polymerization (MIP) of FpCX Fp (1) and PR2 CY PR2 (2) (Fp: CpFe(CO)2 ; CX and CY : alkyl spacers; P: phosphine; R: phenyl or isopropyl) generates P(1/2), in which the P-Fe and Fe-P bonds with opposite bonding direction are alternatively arranged in the backbone. On the other hand, P(FpCX P) synthesized from AB-type monomers (FpCX P) has P-Fe bonds arranged in the same direction. P(1/2) is more rigid and stable than P(FpCX P), which is attributed to the chain conformation resulting from the P-Fe bonding direction. In addition, the longer spacers render P(1/2) relatively flexible; the phenyl substituents, as compared with the isopropyl groups, improves the rigidity, thermal, and solution stability of P(1/2). It is therefore possible to incorporate weak metal coordination bonds into macromolecules with improved stability and adjustable flexibility for material processing. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. A general method to study equilibrium partitioning of macromolecules

    DEFF Research Database (Denmark)

    The distribution of macromolecules between a confined microscopic solution and a macroscopic bulk solution plays an important role in understanding separation processes such as Size Exclusion Chromatography (SEC). In this study, we have developed an efficient computational algorithm for obtaining...... the equilibrium partition coefficient (pore-to-bulk concentration ratio) and the concentration profile inside the confining geometry. The algorithm involves two steps. First, certain characteristic structure properties of the studied macromolecule are obtained by sampling its configuration space, and second those...... data are used for the computation of partition coefficient and concentration profile for any confinement size. Our algorithm is versatile to the model and type of the macromolecule studied, and is capable of handling three types of confining geometries (slit, rectangular channel and rectangular box...

  3. Nonlinear Model Algorithmic Control of a pH Neutralization Process

    Institute of Scientific and Technical Information of China (English)

    ZOU Zhiyun; YU Meng; WANG Zhizhen; LIU Xinghong; GUO Yuqing; ZHANG Fengbo; GUO Ning


    Control of pH neutralization processes is challenging in the chemical process industry because of their inherent strong nonlinearity.In this paper,the model algorithmic control (MAC) strategy is extended to nonlinear processes using Hammerstein model that consists of a static nonlinear polynomial function followed in series by a linear impulse response dynamic element.A new nonlinear Hammerstein MAC algorithm (named NLH-MAC) is presented in detail.The simulation control results of a pH neutralization process show that NLH-MAC gives better control performance than linear MAC and the commonly used industrial nonlinear propotional plus integral plus derivative (PID) controller.Further simulation experiment demonstrates that NLH-MAC not only gives good control response,but also possesses good stability and robustness even with large modeling errors.

  4. Adsorption of charged macromolecules on a gold electrode

    NARCIS (Netherlands)

    Barten, D.


    In this thesis we have examined the role of electrostatic interactions in the adsorption of charged macromolecules from aqueous solution to a solid surface and the possibilitiesfor manipulatingthis process through the electric potential

  5. Bioenergetic aspects of the translocation of macromolecules across bacterial membranes

    NARCIS (Netherlands)

    Palmen, Ronald; Driessen, Arnold J.M.; Hellingwerf, K


    Bacteria are extremely versatile in the sense that they have gained the ability to transport all three major classes of biopolymers through their cell envelope: proteins, nucleic acids, and polysaccharides. These macromolecules are translocated across membranes in a large number of cellular processe

  6. Femtosecond Fiber Lasers Based on Dissipative Processes for Nonlinear Microscopy (United States)

    Wise, Frank W.


    Recent progress in the development of femtosecond-pulse fiber lasers with parameters appropriate for nonlinear microscopy is reviewed. Pulse-shaping in lasers with only normal-dispersion components is briefly described, and the performance of the resulting lasers is summarized. Fiber lasers based on the formation of dissipative solitons now offer performance competitive with that of solid-state lasers, but with the benefits of the fiber medium. Lasers based on self-similar pulse evolution in the gain section of a laser also offer a combination of short pulse duration and high pulse energy that will be attractive for applications in nonlinear bioimaging. PMID:23869163

  7. Configurational diffusion of coal macromolecules

    Energy Technology Data Exchange (ETDEWEB)

    Guin, J.A.; Curtis, C.W.; Tarrer, A.R.; Kim, S.; Hwang, D.; Chen, C.C.; Chiou, Z.


    The objective of our research was to obtain fundamental information regarding the functional dependence of the diffusion coefficient of coal molecules on the ratio of molecule to pore diameter. That is, the objective of our study was to examine the effect of molecule size and configuration on hindered diffusion of coal macromolecules through as porous medium. To best accomplish this task, we circumvented the complexities of an actual porous catalyst by using a well defined porous matrix with uniform capillaric pores, i.e., a track-etched membrane. In this way, useful information was obtained regarding the relationship of molecular size and configuration on the diffusion rate of coal derived macromolecules through a pore structure with known geometry. Similar studies were performed using a pellet formed of porous alumina, to provide a link between the idealized membranes and the actual complex pore structure of real catalyst extrudates. The fundamental information from our study will be useful toward the tailoring of catalysts to minimize diffusional influences and thereby increase coal conversion and selectivity for desirable products. (VC)

  8. Mathematical models of non-linear phenomena, processes and systems: from molecular scale to planetary atmosphere

    CERN Document Server


    This book consists of twenty seven chapters, which can be divided into three large categories: articles with the focus on the mathematical treatment of non-linear problems, including the methodologies, algorithms and properties of analytical and numerical solutions to particular non-linear problems; theoretical and computational studies dedicated to the physics and chemistry of non-linear micro-and nano-scale systems, including molecular clusters, nano-particles and nano-composites; and, papers focused on non-linear processes in medico-biological systems, including mathematical models of ferments, amino acids, blood fluids and polynucleic chains.

  9. Nonlinear giant magnetoimpedance and the asymmetric circumferential magnetization process in soft magnetic wires (United States)

    Gómez-Polo, C.; Duque, J. G. S.; Knobel, M.


    The magnetoimpedance effect and its nonlinear terms are analysed for a (Co0.94Fe0.06)72.5Si12.5B15 amorphous wire. In order to enhance the nonlinear contribution the sample was previously subjected to current annealing (Joule heating) to induce a circumferential anisotropy. The effect of the application of a torsional strain on the nonlinear magnetoimpedance is analysed in terms of the torsional dependence of the magnetic permeability, evaluated through experimental circumferential hysteresis loops. The results obtained clearly confirm the direct correlation between the asymmetric circumferential magnetization process and the occurrence of nonlinear second-harmonic terms in the magnetoimpedance voltage.

  10. Neural Generalized Predictive Control of a non-linear Process

    DEFF Research Database (Denmark)

    Sørensen, Paul Haase; Nørgård, Peter Magnus; Ravn, Ole


    qualities. The controller is a non-linear version of the well-known generalized predictive controller developed in linear control theory. It involves minimization of a cost function which in the present case has to be done numerically. Therefore, we develop the numerical algorithms necessary in substantial...

  11. Second Harmonic Light Scattering from Macromolecules: Collagen. (United States)

    Roth, Shmuel

    In this work we present the theory and practice of optical second harmonic generation (SHG) as applied to rat-tail tendon collagen. Our work is the first quantitative application of SHG to biological systems. The angular dependence of SHG is found to display a sharp, intense, forward peak superimposed on a broad background. The sharp peak is shown to imply long-range polar order, while the broad background corresponds to that predicted for the random "up"/"down" array of collagen fibrils seen with the electron microscope. The dependence of fibril diameter distribution on age and state of hydration is measured. Our experiments also reveal information concerning the structure of the fibrils and their arrangement in the tendon. The degree of polar order, the coherence length of tendon for harmonic generation and the absolute magnitude of the nonlinear susceptibility of the collagen fibril are also determined. The biological significance of these findings and the many advantages of SHG for the structural study of biological macromolecules and tissues are discussed.

  12. Modeling and time-dependent dynamics of processes of stimulated depolymerization, auto-repairing, degradation and radiation curing of DNA macromolecules and biopolymers at separated and combined actions of ionizing irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Vysotskii, Vladimir I. E-mail:; Pinchuk, Anatoliy O.; Kornilova, Alla A.; Samoylenko, Igor I


    The time-dependent dynamics of the formation, relaxation and auto-repairing of double breaks of DNA macromolecules at the combined radiation action and non-radiation processes of degradation (e.g. by free radicals) were considered. The auto-repairing of DNA double breaks is connected with the peculiarities of long-range interaction of nucleotide charges, atoms and molecules in the intracellular milieu. The properties of intracellular liquid and the characteristics of force interaction between the end-pairs of nucleotides in the area of DNA break in response to radiation are changed. Each kind of radiation is characterized by a certain effectiveness of the double DNA break formation but simultaneously one creates the conditions for their liquidation. On the basis of the analysis and correlation of these processes the time-dependent theory for DNA degradation was created, including hormesis phenomenon, radiation antagonism, the validity of anomaly influence of low and large doses at sharp and chronic radiation and other effects. The qualitative and quantitative correspondences of the theory and experimental results of radiation biology were obtained.

  13. Nonlinear analysis and control of a continuous fermentation process

    DEFF Research Database (Denmark)

    Szederkényi, G.; Kristensen, Niels Rode; Hangos, K.M


    open-loop system properties, to explore the possible control difficulties and to select the system output to be used in the control structure. A wide range of controllers are tested including pole placement and LQ controllers, feedback and input–output linearization controllers and a nonlinear...... controller based on direct passivation. The comparison is based on time-domain performance and on investigating the stability region, robustness and tuning possibilities of the controllers. Controllers using partial state feedback of the substrate concentration and not directly depending on the reaction rate...... are recommended for the simple fermenter. Passivity based controllers have been found to be globally stable, not very sensitive to the uncertainties in the reaction rate and controller parameter but they require full nonlinear state feedback....

  14. Photonic Crystal Nanocavity Devices for Nonlinear Signal Processing

    DEFF Research Database (Denmark)

    Yu, Yi

    , membranization of InP/InGaAs structure and wet etching. Experimental investigation of the switching dynamics of InP photonic crystal nanocavity structures are carried out using short-pulse homodyne pump-probe techniques, both in the linear and nonlinear region where the cavity is perturbed by a relatively small......This thesis deals with the investigation of InP material based photonic crystal cavity membrane structures, both experimentally and theoretically. The work emphasizes on the understanding of the physics underlying the structures’ nonlinear properties and their applications for all-optical signal...... and large pump power. The experimental results are compared with coupled mode equations developed based on the first order perturbation theory, and carrier rate equations we established for the dynamics of the carrier density governing the cavity properties. The experimental observations show a good...

  15. Neural Generalized Predictive Control of a non-linear Process

    DEFF Research Database (Denmark)

    Sørensen, Paul Haase; Nørgård, Peter Magnus; Ravn, Ole


    The use of neural network in non-linear control is made difficult by the fact the stability and robustness is not guaranteed and that the implementation in real time is non-trivial. In this paper we introduce a predictive controller based on a neural network model which has promising stability...... detail and discuss the implementation difficulties. The neural generalized predictive controller is tested on a pneumatic servo sys-tem....

  16. Star-Shaped Macromolecules with the Core of Hexakis-(fluoren-2-yl)benzene and the Periphery of Pyridine: Synthesis and Application as Solution-Processable Electron-Transport Materials. (United States)

    Yin, Xiaojun; Miao, Jingsheng; Xiang, Yepeng; Wu, Hongbing; Cao, Yong; Yang, Chuluo


    Three new star-shaped macromolecules with hexakis(fluoren-2-yl)benzene as the core and pyridine as the periphery (2Py-HFB, 3Py-HFB, and 4Py-HFB) are synthesized and characterized. The synthetic conditions of octacarbonyldicobat-catalyzed cycloaddition reaction for different alkyne precursors are investigated. The coordination interaction between the pyridine ring of alkyne precursor and the cobalt catalyst may result in very low yield of the cyclotrimerization product. However, with the increase of the catalyst loading, the yields of the intermediates of cyclopentadienone are enhanced. Then, the desired cyclotrimerization products can be obtained by the Diels-Alder reactions of cyclopentadienone with acetylene in good yield. These new compounds exhibit good thermal stability and favorable electron affinity. By using the new compounds as electron-transporting materials, all-solution-processed phosphorescent organic light-emitting devices (OLEDs) show good performance with a maximum current efficiency of 5.6 cd A(-1) and maximum external quantum efficiency of 4.68%. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Antimicrobial resistance challenged with metal-based antimicrobial macromolecules. (United States)

    Abd-El-Aziz, Alaa S; Agatemor, Christian; Etkin, Nola


    Antimicrobial resistance threatens the achievements of science and medicine, as it deactivates conventional antimicrobial therapeutics. Scientists respond to the threat by developing new antimicrobial platforms to prevent and treat infections from these resistant strains. Metal-based antimicrobial macromolecules are emerging as an alternative to conventional platforms because they combine multiple mechanisms of action into one platform due to the distinctive properties of metals. For example, metals interact with intracellular proteins and enzymes, and catalyse various intracellular processes. The macromolecular architecture offers a means to enhance antimicrobial activity since several antimicrobial moieties can be conjugated to the scaffold. Further, these macromolecules can be fabricated into antimicrobial materials for contact-killing medical implants, fabrics, and devices. As volatilization or leaching out of the antimicrobial moieties from the macromolecular scaffold is reduced, these medical implants, fabrics, and devices can retain their antimicrobial activity over an extended period. Recent advances demonstrate the potential of metal-based antimicrobial macromolecules as effective platforms that prevent and treat infections from resistant strains. In this review these advances are thoroughly discussed within the context of examples of metal-based antimicrobial macromolecules, their mechanisms of action and biocompatibility. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Real-Time Implementation of Nonlinear Optical Processing Functions. (United States)


    demonstrating that the memory is nonlinear and selective. The recording medium could be replaced with real-time media such as photorefractive crystals. Thicker...recording media Fi4 4. Schematic of experiment that d,.non* trated ,,pera have the added advantage of higher angular selectiv- "" . e e r aity. thus... geometrica snapes in contact ’A,.n a c-:’:ser ’Figure 51a’ ., and a spher:cal reference -eam Upion :"um’latlon of t -" c-’gram by the object beam

  19. Approaches to handle nonlinearities and nonnormalities in process chemometrics

    NARCIS (Netherlands)

    Thissen, Uwe Maria Johannes


    For every industrial process, it is of paramount interest to online monitor the performance of the process and to assess the quality of the products made. In order to meet these goals, the field of process control works on understanding and improving industrial processes. Process chemometrics can be

  20. Relaxation Dynamics of Semiflexible Fractal Macromolecules

    Directory of Open Access Journals (Sweden)

    Jonas Mielke


    Full Text Available We study the dynamics of semiflexible hyperbranched macromolecules having only dendritic units and no linear spacers, while the structure of these macromolecules is modeled through T-fractals. We construct a full set of eigenmodes of the dynamical matrix, which couples the set of Langevin equations. Based on the ensuing relaxation spectra, we analyze the mechanical relaxation moduli. The fractal character of the macromolecules reveals itself in the storage and loss moduli in the intermediate region of frequencies through scaling, whereas at higher frequencies, we observe the locally-dendritic structure that is more pronounced for higher stiffness.

  1. A nonlinear optoelectronic filter for electronic signal processing (United States)

    Loh, William; Yegnanarayanan, Siva; Ram, Rajeev J.; Juodawlkis, Paul W.


    The conversion of electrical signals into modulated optical waves and back into electrical signals provides the capacity for low-loss radio-frequency (RF) signal transfer over optical fiber. Here, we show that the unique properties of this microwave-photonic link also enable the manipulation of RF signals beyond what is possible in conventional systems. We achieve these capabilities by realizing a novel nonlinear filter, which acts to suppress a stronger RF signal in the presence of a weaker signal independent of their separation in frequency. Using this filter, we demonstrate a relative suppression of 56 dB for a stronger signal having a 1-GHz center frequency, uncovering the presence of otherwise undetectable weaker signals located as close as 3.5 Hz away. The capabilities of the optoelectronic filter break the conventional limits of signal detection, opening up new possibilities for radar and communication systems, and for the field of precision frequency metrology. PMID:24402418

  2. Nonlinear model predictive control for chemical looping process

    Energy Technology Data Exchange (ETDEWEB)

    Joshi, Abhinaya; Lei, Hao; Lou, Xinsheng


    A control system for optimizing a chemical looping ("CL") plant includes a reduced order mathematical model ("ROM") that is designed by eliminating mathematical terms that have minimal effect on the outcome. A non-linear optimizer provides various inputs to the ROM and monitors the outputs to determine the optimum inputs that are then provided to the CL plant. An estimator estimates the values of various internal state variables of the CL plant. The system has one structure adapted to control a CL plant that only provides pressure measurements in the CL loops A and B, a second structure adapted to a CL plant that provides pressure measurements and solid levels in both loops A, and B, and a third structure adapted to control a CL plant that provides full information on internal state variables. A final structure provides a neural network NMPC controller to control operation of loops A and B.

  3. High-Order Volterra Model Predictive Control and Its Application to a Nonlinear Polymerisation Process

    Institute of Scientific and Technical Information of China (English)

    Yun Li; Hiroshi Kashiwagi


    Model Predictive Control (MPC) has recently found wide acceptance in the process industry, but existing design and implementation methods are restricted to linear process models. A chemical process, however, involves severe nonlinearity which cannot be ignored in practice. This paper aims to solve this nonlinear control problem by extending MPC to accommodate nonlinear models. It develops an analytical framework for nonlinear model predictive control (NMPC). It also offers a third-order Volterra series based nonparametric nonlinear modelling technique for NMPC design, which relieves practising engineers from the need for deriving a physical-principles based model first. An on-line realisation technique for implementing NMPC is then developed and applied to a Mitsubishi Chemicals polymerisation reaction process. Results show that this nonlinear MPC technique is feasible and very effective. It considerably outperforms linear and low-order Volterra model based methods. The advantages of the developed approach lie not only in control performance superior to existing NMPC methods, but also in eliminating the need for converting an analytical model and then convert it to a Volterra model obtainable only up to the second order.


    Computational Methods for Studying the Interaction between Polycyclic Aromatic Hydrocarbons and Biological Macromolecules .The mechanisms for the processes that result in significant biological activity of PAHs depend on the interaction of these molecules or their metabol...

  5. Optical pulse engineering and processing using optical nonlinearities of nanostructured waveguides made of silicon (United States)

    Lavdas, Spyros; You, Jie; Osgood, Richard M.; Panoiu, Nicolae C.


    We present recent results pertaining to pulse reshaping and optical signal processing using optical nonlinearities of silicon-based tapered photonic wires and photonic crystal waveguides. In particular, we show how nonlinearity and dispersion engineering of tapered photonic wires can be employed to generate optical similaritons and achieve more than 10× pulse compression. We also discuss the properties of four-wave mixing pulse amplification and frequency conversion efficiency in long-period Bragg waveguides and photonic crystal waveguides. Finally, the influence of linear and nonlinear optical effects on the transmission bit-error rate in uniform photonic wires and photonic crystal waveguides made of silicon is discussed.

  6. Blind Image Deblurring Driven by Nonlinear Processing in the Edge Domain

    Directory of Open Access Journals (Sweden)

    Stefania Colonnese


    Full Text Available This work addresses the problem of blind image deblurring, that is, of recovering an original image observed through one or more unknown linear channels and corrupted by additive noise. We resort to an iterative algorithm, belonging to the class of Bussgang algorithms, based on alternating a linear and a nonlinear image estimation stage. In detail, we investigate the design of a novel nonlinear processing acting on the Radon transform of the image edges. This choice is motivated by the fact that the Radon transform of the image edges well describes the structural image features and the effect of blur, thus simplifying the nonlinearity design. The effect of the nonlinear processing is to thin the blurred image edges and to drive the overall blind restoration algorithm to a sharp, focused image. The performance of the algorithm is assessed by experimental results pertaining to restoration of blurred natural images.

  7. Data Analysis Techniques for Resolving Nonlinear Processes in Plasmas : a Review


    de Wit, T. Dudok


    The growing need for a better understanding of nonlinear processes in plasma physics has in the last decades stimulated the development of new and more advanced data analysis techniques. This review lists some of the basic properties one may wish to infer from a data set and then presents appropriate analysis techniques with some recent applications. The emphasis is put on the investigation of nonlinear wave phenomena and turbulence in space plasmas.

  8. Simulations of the Ocean Response to a Hurricane: Nonlinear Processes

    KAUST Repository

    Zedler, Sarah E.


    Superinertial internal waves generated by a tropical cyclone can propagate vertically and laterally away from their local generation site and break, contributing to turbulent vertical mixing in the deep ocean and maintenance of the stratification of the main thermocline. In this paper, the results of a modeling study are reported to investigate the mechanism by which superinertial fluctuations are generated in the deep ocean. The general properties of the superinertial wave wake were also characterized as a function of storm speed and central latitude. The Massachusetts Institute of Technology (MIT) Ocean General Circulation Model (OGCM) was used to simulate the open ocean response to realistic westward-tracking hurricane-type surface wind stress and heat and net freshwater buoyancy forcing for regions representative of midlatitudes in the Atlantic, the Caribbean, and low latitudes in the eastern Pacific. The model had high horizontal [Δ(x, y) = 1/6°] and vertical (Δz = 5 m in top 100 m) resolution and employed a parameterization for vertical mixing induced by shear instability. In the horizontal momentum equation, the relative size of the nonlinear advection terms, which had a dominant frequency near twice the inertial, was large only in the upper 200 m of water. Below 200 m, the linear momentum equations obeyed a linear balance to 2%. Fluctuations at nearly twice the inertial frequency (2f) were prevalent throughout the depth of the water column, indicating that these nonlinear advection terms in the upper 200 m forced a linear mode below at nearly twice the inertial frequency via vorticity conservation. Maximum variance at 2f in horizontal velocity occurred on the south side of the track. This was in response to vertical advection of northward momentum, which in the north momentum equation is an oscillatory positive definite term that constituted a net force to the south at a frequency near 2f. The ratio of this term to the Coriolis force was larger on the

  9. Nonlinearities in the quantum measurement process of superconducting qubits

    Energy Technology Data Exchange (ETDEWEB)

    Serban, Ioana


    The work described in this thesis focuses on the investigation of decoherence and measurement backaction, on the theoretical description of measurement schemes and their improvement. The study presented here is centered around quantum computing implementations using superconducting devices and most important, the Josephson effect. The measured system is invariantly a qubit, i. e. a two-level system. The objective is to study detectors with increasing nonlinearity, e. g. coupling of the qubit to the frequency a driven oscillator, or to the bifurcation amplifier, to determine the performance and backaction of the detector on the measured system and to investigate the importance of a strong qubit-detector coupling for the achievement of a quantum non-demolition type of detection. The first part gives a very basic introduction to quantum information, briefly reviews some of the most promising physical implementations of a quantum computer before focusing on the superconducting devices. The second part presents a series of studies of different qubit measurements, describing the backaction of the measurement onto the measured system and the internal dynamics of the detector. Methodology adapted from quantum optics and chemical physics (master equations, phase-space analysis etc.) combined with the representation of a complex environment yielded a tool capable of describing a nonlinear, non-Markovian environment, which couples arbitrarily strongly to the measured system. This is described in chapter 3. Chapter 4 focuses on the backaction on the qubit and presents novel insights into the qubit dephasing in the strong coupling regime. Chapter 5 uses basically the same system and technical tools to explore the potential of a fast, strong, indirect measurement, and determine how close such a detection would ideally come to the quantum non-demolition regime. Chapter 6 focuses on the internal dynamics of a strongly driven Josephson junction. The analytical results are based on

  10. Correntropy-based partial directed coherence for testing multivariate Granger causality in nonlinear processes (United States)

    Kannan, Rohit; Tangirala, Arun K.


    Identification of directional influences in multivariate systems is of prime importance in several applications of engineering and sciences such as plant topology reconstruction, fault detection and diagnosis, and neurosciences. A spectrum of related directionality measures, ranging from linear measures such as partial directed coherence (PDC) to nonlinear measures such as transfer entropy, have emerged over the past two decades. The PDC-based technique is simple and effective, but being a linear directionality measure has limited applicability. On the other hand, transfer entropy, despite being a robust nonlinear measure, is computationally intensive and practically implementable only for bivariate processes. The objective of this work is to develop a nonlinear directionality measure, termed as KPDC, that possesses the simplicity of PDC but is still applicable to nonlinear processes. The technique is founded on a nonlinear measure called correntropy, a recently proposed generalized correlation measure. The proposed method is equivalent to constructing PDC in a kernel space where the PDC is estimated using a vector autoregressive model built on correntropy. A consistent estimator of the KPDC is developed and important theoretical results are established. A permutation scheme combined with the sequential Bonferroni procedure is proposed for testing hypothesis on absence of causality. It is demonstrated through several case studies that the proposed methodology effectively detects Granger causality in nonlinear processes.

  11. Solving method of generalized nonlinear dynamic least squares for data processing in building of digital mine

    Institute of Scientific and Technical Information of China (English)

    TAO Hua-xue (陶华学); GUO Jin-yun (郭金运)


    Data are very important to build the digital mine. Data come from many sources, have different types and temporal states. Relations between one class of data and the other one, or between data and unknown parameters are more nonlinear. The unknown parameters are non-random or random, among which the random parameters often dynamically vary with time. Therefore it is not accurate and reliable to process the data in building the digital mine with the classical least squares method or the method of the common nonlinear least squares. So a generalized nonlinear dynamic least squares method to process data in building the digital mine is put forward. In the meantime, the corresponding mathematical model is also given. The generalized nonlinear least squares problem is more complex than the common nonlinear least squares problem and its solution is more difficultly obtained because the dimensions of data and parameters in the former are bigger. So a new solution model and the method are put forward to solve the generalized nonlinear dynamic least squares problem. In fact, the problem can be converted to two sub-problems, each of which has a single variable. That is to say, a complex problem can be separated and then solved. So the dimension of unknown parameters can be reduced to its half, which simplifies the original high dimensional equations. The method lessens the calculating load and opens up a new way to process the data in building the digital mine, which have more sources, different types and more temporal states.

  12. Bond Tension in Tethered Macromolecules. (United States)

    Sheiko, Sergei S; Panyukov, Sergey; Rubinstein, Michael


    The paper presents scaling analysis of mechanical tension generated in densely branched macromolecules tethered to a solid substrate with a short linker. Steric repulsion between branches results in z-fold amplification of tension in the linker, where z is the number of chain-like arms. At large z ~ 100-1000, the generated tension may exceed the strength of covalent bonds and sever the linker. Two types of molecular architectures were considered: polymer stars and polymer "bottlebrushes" tethered to a solid substrate. Depending on the grafting density, one distinguishes the so-called mushroom, loose grafting, and dense grafting regimes. In isolated (mushroom) and loosely tethered bottlebrushes, the linker tension is by a factor of [Formula: see text] smaller than the tension in a tethered star with the same number of arms z. In densely tethered stars, the effect of interchain distance (d) and number of arms (z) on the magnitude of linker tension is given by f ≅ f0z(3/2)(b/d) for stars in a solvent environment and f ≅ f0z(2) (b/d)(2) for dry stars, where b is the Kuhn length and f0 ≅ kBT/b is intrinsic bond tension. These relations are also valid for tethered bottlebrushes with long side chains. However, unlike molecular stars, bottlebrushes demonstrate variation of tension along the backbone f ≅ f0s z(1/2) / d as a function of distance s from the free end of the backbone. In dense brushes [Formula: see text] with z ≅ 1000, the backbone tension increases from f ≅ f0 = 1 pN at the free end of the backbone (s ≅ b) to its maximum f ≅ zf0 ≅ 1 nN at the linker to the substrate (s ≅ zb).

  13. Nonlinear enhancement in photonic crystal slow light waveguides fabricated using CMOS-compatible process. (United States)

    Shinkawa, Mizuki; Ishikura, Norihiro; Hama, Yosuke; Suzuki, Keijiro; Baba, Toshihiko


    We have studied low-dispersion slow light and its nonlinear enhancement in photonic crystal waveguides. In this work, we fabricated the waveguides using Si CMOS-compatible process. It enables us to integrate spotsize converters, which greatly simplifies the optical coupling from fibers as well as demonstration of the nonlinear enhancement. Two-photon absorption, self-phase modulation and four-wave mixing were observed clearly for picosecond pulses in a 200-μm-long device. In comparison with Si wire waveguides, a 60-120 fold higher nonlinearity was evaluated for a group index of 51. Unique intensity response also occurred due to the specific transmission spectrum and enhanced nonlinearities. Such slow light may add various functionalities in Si photonics, while loss reduction is desired for ensuring the advantage of slow light.

  14. Nonlinear Transport Processes in Tokamak Plasmas. Part I: The Collisional Regimes

    CERN Document Server

    Sonnino, Giorgio


    An application of the thermodynamic field theory (TFT) to transport processes in L-mode tokamak plasmas is presented. The nonlinear corrections to the linear (Onsager) transport coefficients in the collisional regimes are derived. A quite encouraging result is the appearance of an asymmetry between the Pfirsch-Schlueter (P-S) ion and electron transport coefficients: the latter presents a nonlinear correction, which is absent for the ions, and makes the radial electron coefficients much larger than the former. Explicit calculations and comparisons between the neoclassical results and the TFT predictions for JET plasmas are also reported. We found that the nonlinear electron P-S transport coefficients exceed the values provided by neoclassical theory by a factor, which may be of the order 100. The nonlinear classical coefficients exceed the neoclassical ones by a factor, which may be of order 2. The expressions of the ion transport coefficients, determined by the neoclassical theory in these two regimes, remain...

  15. Polarization dependent dispersion and its impact on optical parametric process in high nonlinear microstructure fibre

    Institute of Scientific and Technical Information of China (English)

    Xiao Li; Zhang Wei; Huang Yi-Dong; Peng Jiang-De


    High nonlinear microstructure fibre (HNMF) is preferred in nonlinear fibre optics, especially in the applications of optical parametric effects, due to its high optical nonlinear coefficient. However, polarization dependent dispersion will impact the nonlinear optical parametric process in HNMFs. In this paper, modulation instability (MI) method is used to measure the polarization dependent dispersion of a piece of commercial HNMF, including the group velocity dispersion, the dispersion slope, the fourth-order dispersion and group birefringence. It also experimentally demonstrates the impact of the polarization dependent dispersion on the continuous wave supercontinuum (SC) generation. On one axis MI sidebands with symmetric frequency dctunings are generated, while on the other axis with larger MI frequency detuning, SC is generated by soliton self-frequency shift.

  16. Innovation as a nonlinear process, the scientometric perspective, and the specification of an 'innovation opportunities explorer'

    NARCIS (Netherlands)

    Leydesdorff, L.; Rotolo, D.; de Nooy, W.


    The process of innovation follows nonlinear patterns across the domains of science, technology, and the economy. Novel bibliometric mapping techniques can be used to investigate and represent distinctive, but complementary perspectives on the innovation process (e.g. ‘demand’ and ‘supply’) as well

  17. Alignment of synaptic vesicle macromolecules with the macromolecules in active zone material that direct vesicle docking. (United States)

    Harlow, Mark L; Szule, Joseph A; Xu, Jing; Jung, Jae Hoon; Marshall, Robert M; McMahan, Uel J


    Synaptic vesicles dock at active zones on the presynaptic plasma membrane of a neuron's axon terminals as a precondition for fusing with the membrane and releasing their neurotransmitter to mediate synaptic impulse transmission. Typically, docked vesicles are next to aggregates of plasma membrane-bound macromolecules called active zone material (AZM). Electron tomography on tissue sections from fixed and stained axon terminals of active and resting frog neuromuscular junctions has led to the conclusion that undocked vesicles are directed to and held at the docking sites by the successive formation of stable connections between vesicle membrane proteins and proteins in different classes of AZM macromolecules. Using the same nanometer scale 3D imaging technology on appropriately stained frog neuromuscular junctions, we found that ∼10% of a vesicle's luminal volume is occupied by a radial assembly of elongate macromolecules attached by narrow projections, nubs, to the vesicle membrane at ∼25 sites. The assembly's chiral, bilateral shape is nearly the same vesicle to vesicle, and nubs, at their sites of connection to the vesicle membrane, are linked to macromolecules that span the membrane. For docked vesicles, the orientation of the assembly's shape relative to the AZM and the presynaptic membrane is the same vesicle to vesicle, whereas for undocked vesicles it is not. The connection sites of most nubs on the membrane of docked vesicles are paired with the connection sites of the different classes of AZM macromolecules that regulate docking, and the membrane spanning macromolecules linked to these nubs are also attached to the AZM macromolecules. We conclude that the luminal assembly of macromolecules anchors in a particular arrangement vesicle membrane macromolecules, which contain the proteins that connect the vesicles to AZM macromolecules during docking. Undocked vesicles must move in a way that aligns this arrangement with the AZM macromolecules for docking

  18. Quasilinear Extreme Learning Machine Model Based Internal Model Control for Nonlinear Process

    Directory of Open Access Journals (Sweden)

    Dazi Li


    Full Text Available A new strategy for internal model control (IMC is proposed using a regression algorithm of quasilinear model with extreme learning machine (QL-ELM. Aimed at the chemical process with nonlinearity, the learning process of the internal model and inverse model is derived. The proposed QL-ELM is constructed as a linear ARX model with a complicated nonlinear coefficient. It shows some good approximation ability and fast convergence. The complicated coefficients are separated into two parts. The linear part is determined by recursive least square (RLS, while the nonlinear part is identified through extreme learning machine. The parameters of linear part and the output weights of ELM are estimated iteratively. The proposed internal model control is applied to CSTR process. The effectiveness and accuracy of the proposed method are extensively verified through numerical results.

  19. A fuzzy model based adaptive PID controller design for nonlinear and uncertain processes. (United States)

    Savran, Aydogan; Kahraman, Gokalp


    We develop a novel adaptive tuning method for classical proportional-integral-derivative (PID) controller to control nonlinear processes to adjust PID gains, a problem which is very difficult to overcome in the classical PID controllers. By incorporating classical PID control, which is well-known in industry, to the control of nonlinear processes, we introduce a method which can readily be used by the industry. In this method, controller design does not require a first principal model of the process which is usually very difficult to obtain. Instead, it depends on a fuzzy process model which is constructed from the measured input-output data of the process. A soft limiter is used to impose industrial limits on the control input. The performance of the system is successfully tested on the bioreactor, a highly nonlinear process involving instabilities. Several tests showed the method's success in tracking, robustness to noise, and adaptation properties. We as well compared our system's performance to those of a plant with altered parameters with measurement noise, and obtained less ringing and better tracking. To conclude, we present a novel adaptive control method that is built upon the well-known PID architecture that successfully controls highly nonlinear industrial processes, even under conditions such as strong parameter variations, noise, and instabilities.

  20. Plasmodesmata: intercellular tunnels facilitating transport of macromolecules in plants. (United States)

    Kragler, Friedrich


    In plants, intercellular structures named plasmodesmata (PD) form a continuous cytoplasmic network between neighboring cells. PD pores provide channels for intercellular symplasmic (cell-to-cell) transport throughout most tissues of the plant body. Cell-defining proteins, such as transcription factors, and regulatory non-coding sequences, such as short interfering RNA, micro RNA, protein-encoding messenger RNAs, viroids, and viral RNA/DNA genomes move via PD channels to adjacent cells. PD-mediated intercellular transport of macromolecules is a regulated process depending on the tissue, developmental stage, and nature of the transported macromolecule. In this review, PD channels and their similarity to tunneling nanotubes present in animals are highlighted. In addition, homeodomain protein movement and cellular components regulating transport are discussed.

  1. Non-linear thermodynamic laws application to soil processes

    Directory of Open Access Journals (Sweden)

    Ilgiz Khabirov


    Full Text Available An attempt has been made to analyze the possibility to use nonequilibrium thermodynamics for the soil dynamic open systemstreatment. Entropy change of such a system and the entropy coming from or going into the outer sphere. In the steady state, dynamic soil-formation processes occur within an organized structure and are characterized by stable parameters close to equilibrium. Accordingly, when examining soil, one can proceed from the conventional thermodynamic equilibrium. However, the matter of Onzager-Prigozhin general phenomenological theory applicability to soil processes is more complicated. To study soil stability it is necessary to go beyond the limits of linear thermodynamics.

  2. Nonlinear processes upon two-photon interband picosecond excitation of PbWO4 crystal (United States)

    Lukanin, V. I.; Karasik, A. Ya


    A new experimental method is proposed to study the dynamics of nonlinear processes occurring upon two-photon interband picosecond excitation of a lead tungstate crystal and upon its excitation by cw probe radiation in a temporal range from several nanoseconds to several seconds. The method is applied to the case of crystal excitation by a sequence of 25 high-power picosecond pulses with a wavelength of 523.5 nm and 633-nm cw probe radiation. Measuring the probe beam transmittance during crystal excitation, one can investigate the influence of two-photon interband absorption and the thermal nonlinearity of the refractive index on the dynamics of nonlinear processes in a wide range of times (from several nanoseconds to several seconds). The time resolution of the measuring system makes it possible to distinguish fast and slow nonlinear processes of electronic or thermal nature, including the generation of a thermal lens and thermal diffusion. An alternative method is proposed to study the dynamics of induced absorption transformation and, therefore, the dynamics of the development of nonlinear rocesses upon degenerate two-photon excitation of the crystal in the absence of external probe radiation.

  3. Nonlinear Optical Signal Processing for Tbit/s Ethernet Applications

    DEFF Research Database (Denmark)

    Oxenløwe, Leif Katsuo; Galili, Michael; Mulvad, Hans Christian Hansen;


    We review recent experimental demonstrations of Tbaud optical signal processing. In particular, we describe a successful 1.28 Tbit/s serial data generation based on single polarization 1.28 Tbaud symbol rate pulses with binary data modulation (OOK) and subsequent all-optical demultiplexing. We also...

  4. Linear and nonlinear optical processing of polymer matrix nanocomposites (United States)

    DeJournett, Travis J.; Han, Karen; Olasov, Lauren R.; Zeng, Fan W.; Lee, Brennan; Spicer, James B.


    This work focuses on the scalable synthesis and processing of nanostructures in polymer matrix nanocomposites (PMNCs) for applications that require photochemical functionality of these nanostructures. An in situ vapor deposition process using various metal and metal oxide precursors has been used to create a range of nanocomposites that display photochromic and photocatalytic behaviors. Under specific processing conditions, these composites consist of discrete nanoparticles distributed uniformly throughout the bulk of an optically transparent polymer matrix. Incorporating other chemical species as supplementary deposition agents in the synthesis process can modify these particles and produce complicated nanostructures with enhanced properties. In particular, work has been carried out to structure nanoparticles using laser irradiation. Starting with metallic or metal oxide nanoparticles in the polymer matrix, localized chemical vapor deposition in the near-particle environment has been carried out using laser irradiation to decompose chemical precursors leading to the formation of secondary structures surrounding the seed nanoparticles. Control of the spatial and temporal characteristics of the excitation source allows for synthesis of nanocomposites with a high degree of control over the location, composition and size of nanoparticles in the matrix and presents the opportunity to produce patterned materials with spatially varying properties.

  5. Discovery of Reversible Crystallization of Macromolecules (United States)

    Wunderlich, Bernhard


    For 10 years "reversing melting" was observed with temperature-modulated differential scanning calorimetry, TMDSC. This reversing melting is the first harmonic response beyond that caused by the heat capacity of a metastable, semicrystalline macromolecular sample. Before one can identify "reversible melting," the calorimeter response must be corrected for loss of linearity, stationarity, frequency, amplitude, and instrument lag, or proper experiment-design must avoid these problems. Using quasi-isothermal TMDSC, the following observations were made [Prog. Polymer Sci. 28 (2003) 383-450]: Equilibrium crystals of polymers may melt at the equilibrium melting-temperature, but crystallization needs supercooling, even in the presence of crystal nuclei, making the overall process irreversible. Metastable, folded-chain crystals of the same molecules also melt irreversibly, however, may have some specific reversibility. Flexible, linear molecules of up to 10 nm length may melt fully reversibly. Macromolecules of less flexibility may lose the ability to melt reversibly. Decoupling of molecular segments, molecular nucleation, segregation of molar masses, rigid amorphous fractions, effects of equilibrium point defects in crystals and glasses, and transition-less ordering and solidification will be discussed in some detail. Supported by NSF, Polymers Program, DMR-0312233, and the Div. of Mat. Sci., BES, of DOE at ORNL, managed by UT-Battelle, LLC, for the U.S. Department of Energy, DOE-AC05-00OR22725.

  6. A Kernel Time Structure Independent Component Analysis Method for Nonlinear Process Monitoring☆

    Institute of Scientific and Technical Information of China (English)

    Lianfang Cai; Xuemin Tian; Ni Zhang


    Kernel independent component analysis (KICA) is a newly emerging nonlinear process monitoring method, which can extract mutually independent latent variables cal ed independent components (ICs) from process var-iables. However, when more than one IC have Gaussian distribution, it cannot extract the IC feature effectively and thus its monitoring performance will be degraded drastical y. To solve such a problem, a kernel time struc-ture independent component analysis (KTSICA) method is proposed for monitoring nonlinear process in this paper. The original process data are mapped into a feature space nonlinearly and then the whitened data are calculated in the feature space by the kernel trick. Subsequently, a time structure independent component analysis algorithm, which has no requirement for the distribution of ICs, is proposed to extract the IC feature. Finally, two monitoring statistics are built to detect process faults. When some fault is detected, a nonlinear fault identification method is developed to identify fault variables based on sensitivity analysis. The proposed monitoring method is applied in the Tennessee Eastman benchmark process. Applications demonstrate the superiority of KTSICA over KICA.

  7. Nonlinear Statistical Process Monitoring Based on Control Charts with Memory Effect and Kernel Independent Component Analysis

    Institute of Scientific and Technical Information of China (English)


    A novel nonlinear combination process monitoring method was proposed based on techniques with memory effect (multivariate exponentially weighted moving average (MEWMA)) and kernel independent component analysis (KICA). The method was developed for dealing with nonlinear issues and detecting small or moderate drifts in one or more process variables with autocorrelation. MEWMA charts use additional information from the past history of the process for keeping the memory effect of the process behavior trend. KICA is a recently developed statistical technique for revealing hidden, nonlinear statistically independent factors that underlie sets of measurements and it is a two-phase algorithm: whitened kernel principal component analysis (KPCA) plus independent component analysis (ICA). The application to the fluid catalytic cracking unit (FCCU) simulated process indicates that the proposed combined method based on MEWMA and KICA can effectively capture the nonlinear relationship and detect small drifts in process variables. Its performance significantly outperforms monitoring method based on ICA, MEWMA-ICA and KICA, especially for long-term performance deterioration.

  8. Electrically controlled nonlinear optical generation and signal processing in plasmonic metamaterials (Conference Presentation) (United States)

    Cai, Wenshan


    Metamaterials have offered not only the unprecedented opportunity to generate unconventional electromagnetic properties that are not found in nature, but also the exciting potential to create customized nonlinear media with tailored high-order effects. Two particularly compelling directions of current interests are active metamaterials, where the optical properties can be purposely manipulated by external stimuli, and nonlinear metamaterials, which enable intensity-dependent frequency conversion of light. By exploring the interaction of these two directions, we leverage the electrical and optical functions simultaneously supported in nanostructured metals and demonstrate electrically-controlled nonlinear processes from photonic metamaterials. We show that a variety of nonlinear optical phenomena, including the wave mixing and the optical rectification, can be purposely modulated by applied voltage signals. In addition, electrically-induced and voltage-controlled nonlinear effects facilitate us to demonstrate the backward phase matching in a negative index material, a long standing prediction in nonlinear metamaterials. Other results to be covered in this talk include photon-drag effect in plasmonic metamaterials and ion-assisted nonlinear effects from metamaterials in electrolytes. Our results reveal a grand opportunity to exploit optical metamaterials as self-contained, dynamic electrooptic systems with intrinsically embedded electrical functions and optical nonlinearities. Reference: L. Kang, Y. Cui, S. Lan, S. P. Rodrigues, M. L. Brongersma, and W. Cai, Nature Communications, 5, 4680 (2014). S. P. Rodrigues and W.Cai, Nature Nanotechnology, 10, 387 (2015). S. Lan, L. Kang, D. T. Schoen, S. P. Rodrigues, Y. Cui, M. L. Brongersma, and W. Cai, Nature Materials, 14, 807 (2015).

  9. The effect of process delay on dynamical behaviors in a self-feedback nonlinear oscillator (United States)

    Yao, Chenggui; Ma, Jun; Li, Chuan; He, Zhiwei


    The delayed feedback loops play a crucial role in the stability of dynamical systems. The effect of process delay in feedback is studied numerically and theoretically in the delayed feedback nonlinear systems including the neural model, periodic system and chaotic oscillator. The process delay is of key importance in determining the evolution of systems, and the rich dynamical phenomena are observed. By introducing a process delay, we find that it can induce bursting electric activities in the neural model. We demonstrate that this novel regime of amplitude death also exists in the parameter space of feedback strength and process delay for the periodic system and chaotic oscillator. Our results extend the effect of process delay in the paper of Zou et al.(2013) where the process delay can eliminate the amplitude death of the coupled nonlinear systems.

  10. Molecular Imprinting of Macromolecules for Sensor Applications. (United States)

    Saylan, Yeşeren; Yilmaz, Fatma; Özgür, Erdoğan; Derazshamshir, Ali; Yavuz, Handan; Denizli, Adil


    Molecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.

  11. Recent Patents in Pulmonary Delivery of Macromolecules. (United States)

    Ray, Animikh; Mandal, Abhirup; Mitra, Ashim K


    Pulmonary delivery is a non-invasive form of delivery that holds tremendous therapeutic promise for topical and systemic administration of several macromolecules. Oral administration of macromolecules has several limitations such as low bioavailability, degradation of drug before reaching circulation and insufficient absorption across intestinal membrane. Administration of macromolecules such as proteins, peptides and nucleic acids via inhalation offers great potential due to the avoidance of first pass metabolism, higher surface area and rapid clinical response. However, delivery of reproducible, uniform and safe doses of inhaled particles remains a major challenge for clinical translation. Recent advances in the fields of biotechnology and particle engineering led to progress in novel pulmonary drug delivery systems. Moreover, significant developments in carriers and delivery devices prevent denaturation of macromolecules and control their release within the lungs. This article reviews the advances in pulmonary drug delivery systems by focusing on the recent patents in delivery of macromolecules. Furthermore, recent patents in gene delivery to the lungs have also been discussed. List of patents included in this review is comprehensive in terms of pulmonary delivery of therapeutics. It includes inventions related to proteins and peptides, DNA therapeutics, siRNA and other genetic materials with therapeutic applications. The diseases targeted by these therapeutic molecules are varied including but not limited to different forms of cancer, respiratory diseases etc.

  12. Macromolecular crowding: Macromolecules friend or foe. (United States)

    Mittal, Shruti; Chowhan, Rimpy Kaur; Singh, Laishram Rajendrakumar


    Cellular interior is known to be densely crowded due to the presence of soluble and insoluble macromolecules, which altogether occupy ~40% of the total cellular volume. This results in altered biological properties of macromolecules. Macromolecular crowding is observed to have both positive and negative effects on protein folding, structure, stability and function. Significant data has been accumulated so far on both the aspects. However, most of the review articles so far have focused on the positive aspect of macromolecular crowding and not much attention has been paid on the deleterious aspect of crowding on macromolecules. In order to have a complete knowledge of the effect of macromolecular crowding on proteins and enzymes, it is important to look into both the aspects of crowding to determine its precise role under physiological conditions. To fill the gap in the understanding of the effect of macromolecular crowding on proteins and enzymes, this review article focuses on the deleterious influence of crowding on macromolecules. Macromolecular crowding is not always good but also has several deleterious effects on various macromolecular properties. Taken together, the properties of biological macromolecules in vivo appears to be finely regulated by the nature and level of the intracellular crowdedness in order to perform their biological functions appropriately. The information provided here gives an understanding of the role played by the nature and level of cellular crowdedness in intensifying and/or alleviating the burden of various proteopathies. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Nonlinear signal processing of electroencephalograms for automated sleep monitoring (United States)

    Wilson, D.; Rowlands, D. D.; James, Daniel A.; Cutmore, T.


    An automated classification technique is desirable to identify the different stages of sleep. In this paper a technique for differentiating the characteristics of each sleep phase has been developed. This is an ideal pre-processor stage for classifying systems such as neural networks. A wavelet based continuous Morlet transform was developed to analyse the EEG signal in both the time and frequency domain. Test results using two 100 epoch EEG test data sets from pre-recorded EEG data are presented. Key rhythms in the EEG signal were identified and classified using the continuous wavelet transform. The wavelet results indicated each sleep phase contained different rhythms and artefacts (noise from muscle movement in the EEG); providing proof that an EEG can be classified accordingly. The coefficients founded by the wavelet transform have been emphasised by statistical techniques. Hypothesis testing was used to highlight major differences between adjacent sleep stages. Various signal processing methods such as power spectrum density and the discrete wavelet transform have been used to emphasise particular characteristics in an EEG. By implementing signal processing methods on an EEG data set specific rules for each sleep stage have been developed suitable for a neural network classification solution.

  14. Non-linear, adaptive array processing for acoustic interference suppression. (United States)

    Hoppe, Elizabeth; Roan, Michael


    A method is introduced where blind source separation of acoustical sources is combined with spatial processing to remove non-Gaussian, broadband interferers from space-time displays such as bearing track recorder displays. This differs from most standard techniques such as generalized sidelobe cancellers in that the separation of signals is not done spatially. The algorithm performance is compared to adaptive beamforming techniques such as minimum variance distortionless response beamforming. Simulations and experiments using two acoustic sources were used to verify the performance of the algorithm. Simulations were also used to determine the effectiveness of the algorithm under various signal to interference, signal to noise, and array geometry conditions. A voice activity detection algorithm was used to benchmark the performance of the source isolation.

  15. A mathematical model for filtration and macromolecule transport across capillary walls. (United States)

    Facchini, L; Bellin, A; Toro, E F


    Metabolic substrates, such as oxygen and glucose, are rapidly delivered to the cells of large organisms through filtration across microvessels walls. Modelling this important process is complicated by the strong coupling between flow and transport equations, which are linked through the osmotic pressure induced by the colloidal plasma proteins. The microvessel wall is a composite media with the internal glycocalyx layer exerting a strong sieving effect on macromolecules, with respect to the external layer composed by the endothelial cells. The physiological structure of the microvessel is represented as the superimposition of two membranes with different properties; the inner membrane represents the glycocalyx, while the outer membrane represents the surrounding endothelial cells. Application of the mass conservation principle and thermodynamic considerations lead to a model composed of two coupled second-order ordinary differential equations for the hydrostatic and osmotic pressures, one, expressing volumetric mass conservation and the other, which is non-linear in the unknown osmotic pressure, expressing macromolecules mass conservation. Despite the complexity of the system, the assumption that the properties of the layers are piece-wise constant allows us to obtain analytical solutions for the two pressures. This solution is in agreement with experimental observations, which contrary to common belief, show that flow reversal cannot occur in steady-state conditions unless the hydrostatic pressure in the lumen drops below physiologically plausible values. The observed variations of the volumetric flux and the solute mass flux in case of a significant reduction of the hydrostatic pressure at the lumen are in qualitative agreement with observed variations during detailed experiments reported in the literature. On the other hand, homogenising the microvessel wall into a single-layer membrane with equivalent properties leads to a very different distribution of

  16. Age and Creative Productivity: Nonlinear Estimation of an Information-Processing Model. (United States)

    Simonton, Dean Keith


    Applied two-step cognitive model to relationship between age and creative productivity. Selected ideation and elaboration rates as information-processing parameters that define mathematical function which describes age curves and specifies their variance across disciplines. Applied non-linear estimation program to further validate model. Despite…

  17. Ultrafast nonlinear all-optical processes in silicon-on-insulator waveguides

    NARCIS (Netherlands)

    Dekker, R.; Usechak, N.; Först, M.; Driessen, A.


    In this review we present an overview of the progress made in recent years in the field of integrated silicon-on-insulator (SOI) waveguide photonics with a strong emphasis on third-order nonlinear optical processes. Although the focus is on simple waveguide structures the utilization of complex stru

  18. Scene matching based on non-linear pre-processing on reference image and sensed image

    Institute of Scientific and Technical Information of China (English)

    Zhong Sheng; Zhang Tianxu; Sang Nong


    To solve the heterogeneous image scene matching problem, a non-linear pre-processing method for the original images before intensity-based correlation is proposed. The result shows that the proper matching probability is raised greatly. Especially for the low S/N image pairs, the effect is more remarkable.

  19. Experimental observations of the characteristics of hot electron and nonlinear processes produced in special material

    Institute of Scientific and Technical Information of China (English)


    Making use of disk targets composed of several peculiar materials (foam Au, foam C8H8)and hohlraum with a special structure, experiments have been done at"Xing Guang - II" laser facility,which study the characteristics of hot electrons and therelated nonlinear processes such as StimulatedRaman Scattering (SRS), Two Plasma Decay (TPD), StimulatedBrillouin Scattering (SBS), etc.

  20. Compound Cue Processing within the Fast and Frugal Heuristics Approach in Nonlinearly Separable Environments (United States)

    Garcia-Retamero, Rocio; Hoffrage, Ulrich; Dieckmann, Anja; Ramos, Manuel


    Three experiments investigated whether participants used Take The Best (TTB) Configural, a fast and frugal heuristic that processes configurations of cues when making inferences concerning which of two alternatives has a higher criterion value. Participants were presented with a compound cue that was nonlinearly separable from its elements. The…

  1. Characterizing time dependent anomalous diffusion process: A survey on fractional derivative and nonlinear models (United States)

    Wei, Song; Chen, Wen; Hon, Y. C.


    This paper investigates the temporal effects in the modeling of flows through porous media and particles transport. Studies will be made among the time fractional diffusion model and two classical nonlinear diffusion models. The effects of the parameters upon the mentioned models have been studied. By simulating the sub-diffusion processes and comparing the numerical results of these models under different boundary conditions, we can conclude that the time fractional diffusion model is more suitable for simulating the sub-diffusion with steady diffusion rate; whereas the nonlinear models are more appropriate for depicting the sub-diffusion under changing diffusion rate.

  2. Analytical investigation of machining chatter by considering the nonlinearity of process damping (United States)

    Ahmadi, Keivan


    In this paper, the well-established problem of self-excited vibrations in machining is revisited to include the nonlinearity of process damping at the tool and workpiece interface. Machining dynamics is modeled using a time-delayed system with nonlinear damping, and the method of averaging is used to obtain the amplitude of the resulting limit cycles. As a result, an analytical relationship is presented to establish the stability charts corresponding with arbitrary limit cycles in machining systems. The presented analytical solutions are verified using experiments and numerical solutions.

  3. 2-D nonlinear IIR-filters for image processing - An exploratory analysis (United States)

    Bauer, P. H.; Sartori, M.


    A new nonlinear IIR filter structure is introduced and its deterministic properties are analyzed. It is shown to be better suited for image processing applications than its linear shift-invariant counterpart. The new structure is obtained from causality inversion of a 2D quarterplane causal linear filter with respect to the two directions of propagation. It is demonstrated, that by using this design, a nonlinear 2D lowpass filter can be constructed, which is capable of effectively suppressing Gaussian or impulse noise without destroying important image information.


    Institute of Scientific and Technical Information of China (English)

    Xiao Huang; Jian Wang; Ling-zhi Zhang; Zhi-gang Cai; Zhao-xi Lianga


    Four phenoxysilicon networks for nonlinear optical (NLO) applications were designed and prepared by an extended sol-gel process without additional H20 and catalyst. All poled polymer network films possess high second-order nonlinear optical coefficients (d33) of 10-?~10-8 esu. The investigation of NLO temporal stability at room temperature and elevated temperature (120°C) indicated that these films exhibit high d33 stability because the orientation of the chromophores are locked in the phenoxysilicon organic/inorganic networks.

  5. 2-D nonlinear IIR-filters for image processing - An exploratory analysis (United States)

    Bauer, P. H.; Sartori, M.


    A new nonlinear IIR filter structure is introduced and its deterministic properties are analyzed. It is shown to be better suited for image processing applications than its linear shift-invariant counterpart. The new structure is obtained from causality inversion of a 2D quarterplane causal linear filter with respect to the two directions of propagation. It is demonstrated, that by using this design, a nonlinear 2D lowpass filter can be constructed, which is capable of effectively suppressing Gaussian or impulse noise without destroying important image information.

  6. General Differential Contact Identities for Macromolecules. (United States)

    Landy, Jonathan; Pincus, P A; Jho, YongSeok


    We discuss general Maxwell identities relating a macromolecule's charge, the forces acting at its surface, and the osmotic pressure of the solution in which it sits. The identities are closely related to the contact value relations that hold for certain special geometries, but are more general. In particular, the Maxwell identities can be applied to any macromolecule geometry, and they hold both within and outside of mean-field theory. Examples illustrate that combining the identities with approximate treatments of screening can often return simple, accurate osmotic pressure estimates.


    Directory of Open Access Journals (Sweden)



    Full Text Available This paper presents a Neural Network based Model Predictive Control (NNMPC strategy to control nonlinear process. Multilayer Perceptron Neural Network (MLP is chosen to represent a Nonlinear Auto Regressive with eXogenous signal (NARX model of a nonlinear system. NARX dynamic model is based on feed-forward architecture and offers good approximation capabilities along with robustness and accuracy. Based on the identified neural model, a generalized predictive control (GPC algorithm is implemented to control the composition in acontinuous stirred tank reactor (CSTR, whose parameters are optimally determined by solving quadratic performance index using well known Levenberg-Marquardt and Quasi-Newton algorithm. NNMPC is tuned by selecting few horizon parameters and weighting factor. The tracking performance of the NNMPC is tested using different amplitude function as a reference signal on CSTR application. Also the robustness and performance is tested in the presence of disturbance on random reference signal.

  8. Hydex Glass and Amorphous Silicon for Integrated Nonlinear Optical Signal Processing

    CERN Document Server

    Morandotti, Roberto


    Photonic integrated circuits that exploit nonlinear optics in order to generate and process signals all-optically have achieved performance far superior to that possible electronically - particularly with respect to speed. Although silicon-on-insulator has been the leading platform for nonlinear optics for some time, its high two-photon absorption at telecommunications wavelengths poses a fundamental limitation. We review the recent achievements based in new CMOS-compatible platforms that are better suited than SOI for nonlinear optics, focusing on amorphous silicon and Hydex glass. We highlight their potential as well as the challenges to achieving practical solutions for many key applications. These material systems have opened up many new capabilities such as on-chip optical frequency comb generation and ultrafast optical pulse generation and measurement.

  9. Nonlinear Pulse Shaping in Fibres for Pulse Generation and Optical Processing

    Directory of Open Access Journals (Sweden)

    Sonia Boscolo


    Full Text Available The development of new all-optical technologies for data processing and signal manipulation is a field of growing importance with a strong potential for numerous applications in diverse areas of modern science. Nonlinear phenomena occurring in optical fibres have many attractive features and great, but not yet fully explored, potential in signal processing. Here, we review recent progress on the use of fibre nonlinearities for the generation and shaping of optical pulses and on the applications of advanced pulse shapes in all-optical signal processing. Amongst other topics, we will discuss ultrahigh repetition rate pulse sources, the generation of parabolic shaped pulses in active and passive fibres, the generation of pulses with triangular temporal profiles, and coherent supercontinuum sources. The signal processing applications will span optical regeneration, linear distortion compensation, optical decision at the receiver in optical communication systems, spectral and temporal signal doubling, and frequency conversion.

  10. Heterogeneous recurrence representation and quantification of dynamic transitions in continuous nonlinear processes (United States)

    Chen, Yun; Yang, Hui


    Many real-world systems are evolving over time and exhibit dynamical behaviors. In order to cope with system complexity, sensing devices are commonly deployed to monitor system dynamics. Online sensing brings the proliferation of big data that are nonlinear and nonstationary. Although there is rich information on nonlinear dynamics, significant challenges remain in realizing the full potential of sensing data for system control. This paper presents a new approach of heterogeneous recurrence analysis for online monitoring and anomaly detection in nonlinear dynamic processes. A partition scheme, named as Q-tree indexing, is firstly introduced to delineate local recurrence regions in the multi-dimensional continuous state space. Further, we design a new fractal representation of state transitions among recurrence regions, and then develop new measures to quantify heterogeneous recurrence patterns. Finally, we develop a multivariate detection method for on-line monitoring and predictive control of process recurrences. Case studies show that the proposed approach not only captures heterogeneous recurrence patterns in the transformed space, but also provides effective online control charts to monitor and detect dynamical transitions in the underlying nonlinear processes.

  11. Complex Nonlinear Behavior in Metabolic Processes: Global Bifurcation Analysis of Escherichia coli Growth on Multiple Substrates

    Directory of Open Access Journals (Sweden)

    Hyun-Seob Song


    Full Text Available The nonlinear behavior of metabolic systems can arise from at least two different sources. One comes from the nonlinear kinetics of chemical reactions in metabolism and the other from nonlinearity associated with regulatory processes. Consequently, organisms at a constant growth rate (as experienced in a chemostat could display multiple metabolic states or display complex oscillatory behavior both with potentially serious implications to process operation. This paper explores the nonlinear behavior of a metabolic model of Escherichia coli growth on mixed substrates with sufficient detail to include regulatory features through the cybernetic postulate that metabolic regulation is the consequence of a dynamic objective function ensuring the organism’s survival. The chief source of nonlinearity arises from the optimal formulation with the metabolic state determined by a convex combination of reactions contributing to the objective function. The model for anaerobic growth of E. coli was previously examined for multiple steady states in a chemostat fed by a mixture of glucose and pyruvate substrates under very specific conditions and experimentally verified. In this article, we explore the foregoing model for nonlinear behavior over the full range of parameters, γ (the fractional concentration of glucose in the feed mixture and D (the dilution rate. The observed multiplicity is in the cybernetic variables combining elementary modes. The results show steady-state multiplicity up to seven. No Hopf bifurcation was encountered, however. Bifurcation analysis of cybernetic models is complicated by the non-differentiability of the cybernetic variables for enzyme activities. A methodology is adopted here to overcome this problem, which is applicable to more complicated metabolic networks.

  12. Circuits and systems based on delta modulation linear, nonlinear and mixed mode processing

    CERN Document Server

    Zrilic, Djuro G


    This book is intended for students and professionals who are interested in the field of digital signal processing of delta-sigma modulated sequences. The overall focus is on the development of algorithms and circuits for linear, non-linear, and mixed mode processing of delta-sigma modulated pulse streams. The material presented here is directly relevant to applications in digital communication, DSP, instrumentation, and control.

  13. Experimental photonic generation of chirped pulses using nonlinear dispersion-based incoherent processing. (United States)

    Rius, Manuel; Bolea, Mario; Mora, José; Ortega, Beatriz; Capmany, José


    We experimentally demonstrate, for the first time, a chirped microwave pulses generator based on the processing of an incoherent optical signal by means of a nonlinear dispersive element. Different capabilities have been demonstrated such as the control of the time-bandwidth product and the frequency tuning increasing the flexibility of the generated waveform compared to coherent techniques. Moreover, the use of differential detection improves considerably the limitation over the signal-to-noise ratio related to incoherent processing.

  14. PlaMoM: a comprehensive database compiles plant mobile macromolecules (United States)

    Guan, Daogang; Yan, Bin; Thieme, Christoph; Hua, Jingmin; Zhu, Hailong; Boheler, Kenneth R.; Zhao, Zhongying; Kragler, Friedrich; Xia, Yiji; Zhang, Shoudong


    In plants, various phloem-mobile macromolecules including noncoding RNAs, mRNAs and proteins are suggested to act as important long-distance signals in regulating crucial physiological and morphological transition processes such as flowering, plant growth and stress responses. Given recent advances in high-throughput sequencing technologies, numerous mobile macromolecules have been identified in diverse plant species from different plant families. However, most of the identified mobile macromolecules are not annotated in current versions of species-specific databases and are only available as non-searchable datasheets. To facilitate study of the mobile signaling macromolecules, we compiled the PlaMoM (Plant Mobile Macromolecules) database, a resource that provides convenient and interactive search tools allowing users to retrieve, to analyze and also to predict mobile RNAs/proteins. Each entry in the PlaMoM contains detailed information such as nucleotide/amino acid sequences, ortholog partners, related experiments, gene functions and literature. For the model plant Arabidopsis thaliana, protein–protein interactions of mobile transcripts are presented as interactive molecular networks. Furthermore, PlaMoM provides a built-in tool to identify potential RNA mobility signals such as tRNA-like structures. The current version of PlaMoM compiles a total of 17 991 mobile macromolecules from 14 plant species/ecotypes from published data and literature. PlaMoM is available at PMID:27924044

  15. The Place of Macromolecules in Freshman Chemistry (United States)

    Wunderlich, Bernhard


    Discusses the inclusion of knowledge on macromolecules into a freshman chemistry course which emphasizes topics in organic chemistry, polymer science and biochemistry, atoms, chemical thermodynamics, and inorganic chemistry. Indicates that the program is the only way to keep chemistry education up to date. (CC)

  16. Macromolecule diffusion and confinement in prokaryotic cells

    NARCIS (Netherlands)

    Mika, Jacek T.; Poolman, Bert

    We review recent observations on the mobility of macromolecules and their spatial organization in live bacterial cells. We outline the major fluorescence microscopy-based methods to determine the mobility and thus the diffusion coefficients (D) of molecules, which is not trivial in small cells. The

  17. Macromolecule diffusion and confinement in prokaryotic cells

    NARCIS (Netherlands)

    Mika, Jacek T.; Poolman, Bert


    We review recent observations on the mobility of macromolecules and their spatial organization in live bacterial cells. We outline the major fluorescence microscopy-based methods to determine the mobility and thus the diffusion coefficients (D) of molecules, which is not trivial in small cells. The

  18. Macromolecule diffusion and confinement in prokaryotic cells

    NARCIS (Netherlands)

    Mika, Jacek T.; Poolman, Bert


    We review recent observations on the mobility of macromolecules and their spatial organization in live bacterial cells. We outline the major fluorescence microscopy-based methods to determine the mobility and thus the diffusion coefficients (D) of molecules, which is not trivial in small cells. The

  19. Real time remaining useful life prediction based on nonlinear Wiener based degradation processes with measurement errors

    Institute of Scientific and Technical Information of China (English)

    唐圣金; 郭晓松; 于传强; 周志杰; 周召发; 张邦成


    Real time remaining useful life (RUL) prediction based on condition monitoring is an essential part in condition based maintenance (CBM). In the current methods about the real time RUL prediction of the nonlinear degradation process, the measurement error is not considered and forecasting uncertainty is large. Therefore, an approximate analytical RUL distribution in a closed-form of a nonlinear Wiener based degradation process with measurement errors was proposed. The maximum likelihood estimation approach was used to estimate the unknown fixed parameters in the proposed model. When the newly observed data are available, the random parameter is updated by the Bayesian method to make the estimation adapt to the item’s individual characteristic and reduce the uncertainty of the estimation. The simulation results show that considering measurement errors in the degradation process can significantly improve the accuracy of real time RUL prediction.

  20. Soft sensor modeling based on variable partition ensemble method for nonlinear batch processes (United States)

    Wang, Li; Chen, Xiangguang; Yang, Kai; Jin, Huaiping


    Batch processes are always characterized by nonlinear and system uncertain properties, therefore, the conventional single model may be ill-suited. A local learning strategy soft sensor based on variable partition ensemble method is developed for the quality prediction of nonlinear and non-Gaussian batch processes. A set of input variable sets are obtained by bootstrapping and PMI criterion. Then, multiple local GPR models are developed based on each local input variable set. When a new test data is coming, the posterior probability of each best performance local model is estimated based on Bayesian inference and used to combine these local GPR models to get the final prediction result. The proposed soft sensor is demonstrated by applying to an industrial fed-batch chlortetracycline fermentation process.

  1. Design and implementation of non-linear image processing functions for CMOS image sensor (United States)

    Musa, Purnawarman; Sudiro, Sunny A.; Wibowo, Eri P.; Harmanto, Suryadi; Paindavoine, Michel


    Today, solid state image sensors are used in many applications like in mobile phones, video surveillance systems, embedded medical imaging and industrial vision systems. These image sensors require the integration in the focal plane (or near the focal plane) of complex image processing algorithms. Such devices must meet the constraints related to the quality of acquired images, speed and performance of embedded processing, as well as low power consumption. To achieve these objectives, low-level analog processing allows extracting the useful information in the scene directly. For example, edge detection step followed by a local maxima extraction will facilitate the high-level processing like objects pattern recognition in a visual scene. Our goal was to design an intelligent image sensor prototype achieving high-speed image acquisition and non-linear image processing (like local minima and maxima calculations). For this purpose, we present in this article the design and test of a 64×64 pixels image sensor built in a standard CMOS Technology 0.35 μm including non-linear image processing. The architecture of our sensor, named nLiRIC (non-Linear Rapid Image Capture), is based on the implementation of an analog Minima/Maxima Unit. This MMU calculates the minimum and maximum values (non-linear functions), in real time, in a 2×2 pixels neighbourhood. Each MMU needs 52 transistors and the pitch of one pixel is 40×40 mu m. The total area of the 64×64 pixels is 12.5mm2. Our tests have shown the validity of the main functions of our new image sensor like fast image acquisition (10K frames per second), minima/maxima calculations in less then one ms.

  2. Macromolecules Inquiry: Transformation of a Standard Biochemistry Lab (United States)

    Unsworth, Elizabeth


    Identification of macromolecules in food is a standard introductory high school biology lab. The intent of this article is to describe the conversion of this standard cookbook lab into an inquiry investigation. Instead of verifying the macromolecules found in food, students use their knowledge of the macromolecules in food to determine the…

  3. A novel thermodynamic state recursion method for description of nonideal nonlinear chromatographic process of frontal analysis. (United States)

    Liu, Qian; OuYang, Liangfei; Liang, Heng; Li, Nan; Geng, Xindu


    A novel thermodynamic state recursion (TSR) method, which is based on nonequilibrium thermodynamic path described by the Lagrangian-Eulerian representation, is presented to simulate the whole chromatographic process of frontal analysis using the spatial distribution of solute bands in time series like as a series of images. TSR differs from the current numerical methods using the partial differential equations in Eulerian representation. The novel method is used to simulate the nonideal, nonlinear hydrophobic interaction chromatography (HIC) processes of lysozyme and myoglobin under the discrete complex boundary conditions. The results show that the simulated breakthrough curves agree well with the experimental ones. The apparent diffusion coefficient and the Langmuir isotherm parameters of the two proteins in HIC are obtained by the state recursion inverse method. Due to its the time domain and Markov characteristics, TSR is applicable to the design and online control of the nonlinear multicolumn chromatographic systems.

  4. Photonic Damascene Process for Integrated High-Q Microresonator Based Nonlinear Photonics

    CERN Document Server

    Pfeiffer, Martin H P; Brasch, Victor; Zervas, Michael; Geiselmann, Michael; Jost, John D; Kippenberg, Tobias J


    High confinement, integrated silicon nitride (SiN) waveguides have recently emerged as attractive platform for on-chip nonlinear optical devices. The fabrication of high-Q SiN microresonators with anomalous group velocity dispersion (GVD) has enabled broadband nonlinear optical frequency comb generation. Such frequency combs have been successfully applied in coherent communication and ultrashort pulse generation. However, the reliable fabrication of high confinement waveguides from stoichiometric, high stress SiN remains challenging. Here we present a novel photonic Damascene fabrication process enabling the use of substrate topography for stress control and thin film crack prevention. With close to unity sample yield we fabricate microresonators with $1.35\\,\\mu\\mathrm{m}$ thick waveguides and optical Q factors of $3.7\\times10^{6}$ and demonstrate single temporal dissipative Kerr soliton (DKS) based coherent optical frequency comb generation. Our newly developed process is interesting also for other material ...

  5. Nonlinear software sensor for monitoring genetic regulation processes with noise and modeling errors

    CERN Document Server

    Ibarra-Junquera, V; Rosu, H C; Arguello, G; Collado-Vides, J


    Nonlinear control techniques by means of a software sensor that are commonly used in chemical engineering could be also applied to genetic regulation processes. We provide here a realistic formulation of this procedure by introducing an additive white Gaussian noise, which is usually found in experimental data. Besides, we include model errors, meaning that we assume we do not know the nonlinear regulation function of the process. In order to illustrate this procedure, we employ the Goodwin dynamics of the concentrations (1963) in the simple form recently discussed by De Jong (2002), which involves the dynamics of the mRNA a, given protein A, and metabolite K concentrations. However instead of considering their full dynamics, we use only the data of metabolite K and a designed software sensor. We also show, more generally, that it is possible to rebuild the complete set of n concentrations despite the uncertainties in the regulation function and the perturbation due to the additive white Gaussian noise

  6. The SPH approach to the process of container filling based on non-linear constitutive models

    Institute of Scientific and Technical Information of China (English)

    Tao Jiang; Jie Ouyang; Lin Zhang; Jin-Lian Ren


    In this work,the transient free surface of container filling with non-linear constitutive equation's fluids is numerically investigated by the smoothed particle hydrodynamics (SPH) method.Specifically,the filling process of a square container is considered for non-linear polymer fluids based on the Cross model.The validity of the presented SPH is first verified by solving the Newtonian fluid and OldroydB fluid jet.Various phenomena in the filling process are shown,including the jet buckling,jet thinning,splashing or spluttering,steady filling.Moreover,a new phenomenon of vortex whirling is more evidently observed for the Cross model fluid compared with the Newtonian fluid case.

  7. High-Accuracy Pneumatic Position Control by Applying Nonlinear Control and Arranging Transient Process

    Institute of Scientific and Technical Information of China (English)

    WANG Yan-bo; BAO Gang


    By applying a nonlinear control and arranging a transient process, the initiative error of the pneumatic servo positioning system is reduced largely, and a larger gain of the controller is used to improve the responding speed of the system at the same damping ratio. Therefore, a compromise is made among the responding speed, overshoot, robustness, adaptability and stability. In addition, a dynamic output feedback controller, including position velocity and acceleration (PVA) feedback, is designed to improve the performance of the system. And a nonlinear controller is reconstructed based on the linear output feedback controller to decrease noises and disturbances. The dynamic responses of the system are simulated and tested. Results show that the error is kept within 0.02 mm under different mass loads and the positioning transient process is smooth, without overshoot and speedy.

  8. Effects of non-linear rheology on the electrospinning process: a model study

    CERN Document Server

    Pontrelli, Giuseppe; Coluzza, Ivan; Pisignano, Dario; Succi, Sauro


    We develop an analytical bead-spring model to investigate the role of non-linear rheology on the dynamics of electrified jets in the early stage of the electrospinning process. Qualitative arguments, parameter studies as well as numerical simulations, show that the elongation of the charged jet filament is significantly reduced in the presence of a non-zero yield stress. This may have beneficial implications for the optimal design of future electrospinning experiments.

  9. Modern meta-heuristics based on nonlinear physics processes: A review of models and design procedures (United States)

    Salcedo-Sanz, S.


    Meta-heuristic algorithms are problem-solving methods which try to find good-enough solutions to very hard optimization problems, at a reasonable computation time, where classical approaches fail, or cannot even been applied. Many existing meta-heuristics approaches are nature-inspired techniques, which work by simulating or modeling different natural processes in a computer. Historically, many of the most successful meta-heuristic approaches have had a biological inspiration, such as evolutionary computation or swarm intelligence paradigms, but in the last few years new approaches based on nonlinear physics processes modeling have been proposed and applied with success. Non-linear physics processes, modeled as optimization algorithms, are able to produce completely new search procedures, with extremely effective exploration capabilities in many cases, which are able to outperform existing optimization approaches. In this paper we review the most important optimization algorithms based on nonlinear physics, how they have been constructed from specific modeling of a real phenomena, and also their novelty in terms of comparison with alternative existing algorithms for optimization. We first review important concepts on optimization problems, search spaces and problems' difficulty. Then, the usefulness of heuristics and meta-heuristics approaches to face hard optimization problems is introduced, and some of the main existing classical versions of these algorithms are reviewed. The mathematical framework of different nonlinear physics processes is then introduced as a preparatory step to review in detail the most important meta-heuristics based on them. A discussion on the novelty of these approaches, their main computational implementation and design issues, and the evaluation of a novel meta-heuristic based on Strange Attractors mutation will be carried out to complete the review of these techniques. We also describe some of the most important application areas, in

  10. Molecular Optics Nonlinear Optical Processes in Organic and Polymeric Crystals and Films. Part 2 (United States)


    susceptibility gamma ijkl(-omega 4; omega 1, omega 2, omega 3 ) demonstrate that the microscopic origin of the nonresonant third order nonlinear optical...interaction calculations of gamma jkl(-omega 4; omega 1, omega 2, omega 3 ) for the archetypal class of quasi-one dimensional conjugated structures...largest of the two dominant, competing virtual excitation processes that determine gamma ijkl(- omega 4; omega 1, omega 2, omega 3 ). It is also found in

  11. Data-driven design of fault diagnosis systems nonlinear multimode processes

    CERN Document Server

    Haghani Abandan Sari, Adel


    In many industrial applications early detection and diagnosis of abnormal behavior of the plant is of great importance. During the last decades, the complexity of process plants has been drastically increased, which imposes great challenges in development of model-based monitoring approaches and it sometimes becomes unrealistic for modern large-scale processes. The main objective of Adel Haghani Abandan Sari is to study efficient fault diagnosis techniques for complex industrial systems using process historical data and considering the nonlinear behavior of the process. To this end, different methods are presented to solve the fault diagnosis problem based on the overall behavior of the process and its dynamics. Moreover, a novel technique is proposed for fault isolation and determination of the root-cause of the faults in the system, based on the fault impacts on the process measurements. Contents Process monitoring Fault diagnosis and fault-tolerant control Data-driven approaches and decision making Target...

  12. Development of coherent tunable source in 2–16 m region using nonlinear frequency mixing processes

    Indian Academy of Sciences (India)

    Udit Chatterjee


    A very convenient way to obtain widely tunable source of coherent radiation in the infrared region is through nonlinear frequency mixing processes like second harmonic generation (SHG), difference-frequency mixing (DFM) or optical parametric oscillation (OPO). Using commonly available Nd:YAG laser and its harmonic pumped dye laser radiation as parent beams, we have been able to generate coherent tunable infrared radiation (IR) in 2–16 m region using different nonlinear crystals by DFM and OPO. We have also generated such IR source in the 4–5 m region through SHG of CO2 laser in different infrared crystals. In the process we have characterized a large number of nonlinear crystals like different borate group of crystals, KTP, KTA, LiIO3, MgO:LiNbO3, GaSe, AgGaSe2, ZnGeP2, AgGa1−InSe2, HgGa2S4 etc. To improve the conversion efficiencies of such frequency conversion processes, we have developed some novel schemes, like multipass configuration (MC) and positive optical feedback (POF). The significance of the obtained results lies in the fact that to get the same conversion in SHG or DFM, one now requires fundamental input radiation with much lower intensity.

  13. Nonlinear Optical Properties of Organic and Polymeric Thin Film Materials of Potential for Microgravity Processing Studies (United States)

    Abdeldayem, Hossin; Frazier, Donald O.; Paley, Mark S.; Penn, Benjamin; Witherow, William K.; Bank, Curtis; Shields, Angela; Hicks, Rosline; Ashley, Paul R.


    In this paper, we will take a closer look at the state of the art of polydiacetylene, and metal-free phthalocyanine films, in view of the microgravity impact on their optical properties, their nonlinear optical properties and their potential advantages for integrated optics. These materials have many attractive features with regard to their use in integrated optical circuits and optical switching. Thin films of these materials processed in microgravity environment show enhanced optical quality and better molecular alignment than those processed in unit gravity. Our studies of these materials indicate that microgravity can play a major role in integrated optics technology. Polydiacetylene films are produced by UV irradiation of monomer solution through an optical window. This novel technique of forming polydiacetylene thin films has been modified for constructing sophisticated micro-structure integrated optical patterns using a pre-programmed UV-Laser beam. Wave guiding through these thin films by the prism coupler technique has been demonstrated. The third order nonlinear parameters of these films have been evaluated. Metal-free phthalocyanine films of good optical quality are processed in our laboratories by vapor deposition technique. Initial studies on these films indicate that they have excellent chemical, laser, and environmental stability. They have large nonlinear optical parameters and show intrinsic optical bistability. This bistability is essential for optical logic gates and optical switching applications. Waveguiding and device making investigations of these materials are underway.

  14. Soft Sensor for Inputs and Parameters Using Nonlinear Singular State Observer in Chemical Processes

    Institute of Scientific and Technical Information of China (English)

    许锋; 汪晔晔; 罗雄麟


    Chemical processes are usually nonlinear singular systems. In this study, a soft sensor using nonlinear singular state observer is established for unknown inputs and uncertain model parameters in chemical processes, which are augmented as state variables. Based on the observability of the singular system, this paper presents a simplified observability criterion under certain conditions for unknown inputs and uncertain model parameters. When the observability is satisfied, the unknown inputs and the uncertain model parameters are estimated online by the soft sensor using augmented nonlinear singular state observer. The riser reactor of fluid catalytic cracking unit is used as an example for analysis and simulation. With the catalyst circulation rate as the only unknown input without model error, one temperature sensor at the riser reactor outlet will ensure the correct estimation for the catalyst cir-culation rate. However, when uncertain model parameters also exist, additional temperature sensors must be used to ensure correct estimation for unknown inputs and uncertain model parameters of chemical processes.

  15. Green functions and Langevin equations for nonlinear diffusion equations: A comment on ‘Markov processes, Hurst exponents, and nonlinear diffusion equations’ by Bassler et al. (United States)

    Frank, T. D.


    We discuss two central claims made in the study by Bassler et al. [K.E. Bassler, G.H. Gunaratne, J.L. McCauley, Physica A 369 (2006) 343]. Bassler et al. claimed that Green functions and Langevin equations cannot be defined for nonlinear diffusion equations. In addition, they claimed that nonlinear diffusion equations are linear partial differential equations disguised as nonlinear ones. We review bottom-up and top-down approaches that have been used in the literature to derive Green functions for nonlinear diffusion equations and, in doing so, show that the first claim needs to be revised. We show that the second claim as well needs to be revised. To this end, we point out similarities and differences between non-autonomous linear Fokker-Planck equations and autonomous nonlinear Fokker-Planck equations. In this context, we raise the question whether Bassler et al.’s approach to financial markets is physically plausible because it necessitates the introduction of external traders and causes. Such external entities can easily be eliminated when taking self-organization principles and concepts of nonextensive thermostatistics into account and modeling financial processes by means of nonlinear Fokker-Planck equations.

  16. Ultrafast nonlinear optical processes in metal-dielectric nanocomposites and nanostructures

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kwang-Hyon


    This work reports results of a theoretical study of nonlinear optical processes in metal-dielectric nanocomposites used for the increase of the nonlinear coefficients and for plasmonic field enhancement. The main results include the study of the transient saturable nonlinearity in dielectric composites doped with metal nanoparticles, its physical mechanism as well its applications in nonlinear optics. For the study of the transient response, a time-depending equation for the dielectric function of the nanocomposite using the semi-classical two-temperature model is derived. By using this approach, we study the transient nonlinear characteristics of these materials in comparison with preceding experimental measurements. The results show that these materials behave as efficient saturable absorbers for passive mode-locking of lasers in the spectral range from the visible to near IR. We present results for the modelocked dynamics in short-wavelength solid-state and semiconductor disk lasers; in this spectral range other efficient saturable absorbers do not exist. We suggest a new mechanism for the realization of slow light phenomenon by using glasses doped with metal nanoparticles in a pump-probe regime near the plasmonic resonance. Furthermore, we study femtosecond plasmon generation by mode-locked surface plasmon polariton lasers with Bragg reflectors and metal-gain-absorber layered structures. In the final part of the thesis, we present results for high-order harmonic generation near a metallic fractal rough surface. The results show a possible reduction of the pump intensities by three orders of magnitudes and two orders of magnitudes higher efficiency compared with preceding experimental results by using bow-tie nanostructures.

  17. Sperm macromolecules associated with bull fertility. (United States)

    Kaya, Abdullah; Memili, Erdoğan


    Bull fertility, ability of the sperm to fertilize and activate the egg that sustain embryo development, is vitally important for effective and efficient production of cattle. Fertility is a complex trait with low heritability. Despite recent advances in genomic selection and possibility of enormous paternal benefits to profitable cattle production, there exist no reliable tests for evaluating semen quality and predicting bull fertility. This review focuses on sperm macromolecules such as transcripts, proteins and the epigenome, i.e., the functional genome that are associated with bull fertility. Generating new information in these systems is important beyond agriculture because such progress advances the fundamental science of the mammalian male gamete while at the same time introduces biotechnology into livestock production. Sperm macromolecules and epigenome markers associated with bull fertility can be used alone or in combination with the current SNP microarrays to determine sperm quality and to indicate bull fertility.

  18. Adaptive optimal control of highly dissipative nonlinear spatially distributed processes with neuro-dynamic programming. (United States)

    Luo, Biao; Wu, Huai-Ning; Li, Han-Xiong


    Highly dissipative nonlinear partial differential equations (PDEs) are widely employed to describe the system dynamics of industrial spatially distributed processes (SDPs). In this paper, we consider the optimal control problem of the general highly dissipative SDPs, and propose an adaptive optimal control approach based on neuro-dynamic programming (NDP). Initially, Karhunen-Loève decomposition is employed to compute empirical eigenfunctions (EEFs) of the SDP based on the method of snapshots. These EEFs together with singular perturbation technique are then used to obtain a finite-dimensional slow subsystem of ordinary differential equations that accurately describes the dominant dynamics of the PDE system. Subsequently, the optimal control problem is reformulated on the basis of the slow subsystem, which is further converted to solve a Hamilton-Jacobi-Bellman (HJB) equation. HJB equation is a nonlinear PDE that has proven to be impossible to solve analytically. Thus, an adaptive optimal control method is developed via NDP that solves the HJB equation online using neural network (NN) for approximating the value function; and an online NN weight tuning law is proposed without requiring an initial stabilizing control policy. Moreover, by involving the NN estimation error, we prove that the original closed-loop PDE system with the adaptive optimal control policy is semiglobally uniformly ultimately bounded. Finally, the developed method is tested on a nonlinear diffusion-convection-reaction process and applied to a temperature cooling fin of high-speed aerospace vehicle, and the achieved results show its effectiveness.

  19. Regulation of synaptic vesicle docking by different classes of macromolecules in active zone material. (United States)

    Szule, Joseph A; Harlow, Mark L; Jung, Jae Hoon; De-Miguel, Francisco F; Marshall, Robert M; McMahan, Uel J


    The docking of synaptic vesicles at active zones on the presynaptic plasma membrane of axon terminals is essential for their fusion with the membrane and exocytosis of their neurotransmitter to mediate synaptic impulse transmission. Dense networks of macromolecules, called active zone material, (AZM) are attached to the presynaptic membrane next to docked vesicles. Electron tomography has shown that some AZM macromolecules are connected to docked vesicles, leading to the suggestion that AZM is somehow involved in the docking process. We used electron tomography on the simply arranged active zones at frog neuromuscular junctions to characterize the connections of AZM to docked synaptic vesicles and to search for the establishment of such connections during vesicle docking. We show that each docked vesicle is connected to 10-15 AZM macromolecules, which fall into four classes based on several criteria including their position relative to the presynaptic membrane. In activated axon terminals fixed during replacement of docked vesicles by previously undocked vesicles, undocked vesicles near vacated docking sites on the presynaptic membrane have connections to the same classes of AZM macromolecules that are connected to docked vesicles in resting terminals. The number of classes and the total number of macromolecules to which the undocked vesicles are connected are inversely proportional to the vesicles' distance from the presynaptic membrane. We conclude that vesicle movement toward and maintenance at docking sites on the presynaptic membrane are directed by an orderly succession of stable interactions between the vesicles and distinct classes of AZM macromolecules positioned at different distances from the membrane. Establishing the number, arrangement and sequence of association of AZM macromolecules involved in vesicle docking provides an anatomical basis for testing and extending concepts of docking mechanisms provided by biochemistry.

  20. Identifiability, reducibility, and adaptability in allosteric macromolecules. (United States)

    Bohner, Gergő; Venkataraman, Gaurav


    The ability of macromolecules to transduce stimulus information at one site into conformational changes at a distant site, termed "allostery," is vital for cellular signaling. Here, we propose a link between the sensitivity of allosteric macromolecules to their underlying biophysical parameters, the interrelationships between these parameters, and macromolecular adaptability. We demonstrate that the parameters of a canonical model of the mSlo large-conductance Ca(2+)-activated K(+) (BK) ion channel are non-identifiable with respect to the equilibrium open probability-voltage relationship, a common functional assay. We construct a reduced model with emergent parameters that are identifiable and expressed as combinations of the original mechanistic parameters. These emergent parameters indicate which coordinated changes in mechanistic parameters can leave assay output unchanged. We predict that these coordinated changes are used by allosteric macromolecules to adapt, and we demonstrate how this prediction can be tested experimentally. We show that these predicted parameter compensations are used in the first reported allosteric phenomena: the Bohr effect, by which hemoglobin adapts to varying pH. © 2017 Bohner and Venkataraman.

  1. Assessment of permeability barriers to macromolecules in the rodent endometrium at the onset of implantation. (United States)

    Bany, Brent M; Hamilton, G Scot


    In rodents, embryo implantation is an invasive process, which begins with its attachment to the uterine wall and culminates in the formation of the definitive placenta several days later. It is critical that the endometrium provide a supportive environment for the implanting embryo during this process, as the placenta is not yet established. The concept of changing permeability barriers to macromolecules between different extracellular compartments in the rodent uterus at the onset of implantation has been established. This chapter provides protocols that can be used to assess this changing permeability barrier and the associated redistribution of macromolecules during the early phases of implantation in rodents. An increased permeability of the endometrial vasculature to plasma proteins occurs in areas adjacent to the implanting blastocyst. In addition, alterations in the extracellular matrix enhance the accumulation of fluid and extravasated macromolecules. We describe several protocols proven to be effective in studying and quantifying early vascular and extravascular responses to natural and artificial "implantation stimuli." The first three protocols represent qualitative and quantitative methods to assess the early endometrial "vascular permeability" response. On the contrary, the fourth protocol addresses the onset of decidualization and the arising permeability barrier, which restricts the movement of macromolecules through the extracellular space. This barrier is believed to provide transient protection for the implanting embryo against potentially harmful maternal serum proteins. This protocol describes assessment of resistance of the primary decidual zone to the movement of macromolecules across the compartments of the extracellular space.

  2. Multivariable adaptive control and estimation of a nonlinear wastewater treatment process

    Energy Technology Data Exchange (ETDEWEB)

    Ben Youssef, C.; Dahhou, B. [Centre National de la Recherche Scientifique (CNRS), 31 - Toulouse (France)]|[Institut National des Sciences Appliquees (INSA), 31 - Toulouse (France)


    In this paper, an approach for estimating biological state and parameter variables and for controlling a non linear wastewater treatment process is developed. Combination of a nonlinear estimation procedure and a multivariable reference model control law provides favourable performances for tracking a given model-based reference model despite disturbances and system parameter uncertainties. Convergence of both estimation and control scheme are demonstrated via Lyapunov`s method. Simulation study with additive measurements noises and parameter jumps shows the efficiency and significant robustness of the control methodology developed for this non linear process. (author) 13 refs.

  3. Hamiltonian models of multiphoton processes and four--photon squeezed states via nonlinear canonical transformations

    CERN Document Server

    De Siena, S; Illuminati, F; Siena, Silvio De; Lisi, Antonio Di; Illuminati, Fabrizio


    We introduce nonlinear canonical transformations that yield effective Hamiltonians of multiphoton down conversion processes, and we define the associated non-Gaussian multiphoton squeezed states as the coherent states of the multiphoton Hamiltonians. We study in detail the four-photon processes and the associated non-Gaussian four-photon squeezed states. The realization of squeezing, the behavior of the field statistics, and the structure of the phase space distributions show that these states realize a natural four-photon generalization of the two-photon squeezed states.

  4. Nonlinear tracking in a diffusion process with a Bayesian filter and the finite element method

    DEFF Research Database (Denmark)

    Pedersen, Martin Wæver; Thygesen, Uffe Høgsbro; Madsen, Henrik


    A new approach to nonlinear state estimation and object tracking from indirect observations of a continuous time process is examined. Stochastic differential equations (SDEs) are employed to model the dynamics of the unobservable state. Tracking problems in the plane subject to boundaries...... become complicated using SMC because Monte Carlo randomness is introduced. The finite element (FE) method solves the Kolmogorov equations of the SDE numerically on a triangular unstructured mesh for which boundary conditions to the state-space are simple to incorporate. The FE approach to nonlinear state...... estimation is suited for off-line data analysis because the computed smoothed state densities, maximum a posteriori parameter estimates and state sequence are deterministic conditional on the finite element mesh and the observations. The proposed method is conceptually similar to existing point...

  5. Nonlinear dynamics of three-magnon process driven by ferromagnetic resonance in yttrium iron garnet

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, R. O. [Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE (Brazil); Centro Interdisciplinar de Ciências da Natureza, Universidade Federal da Integração Latino-Americana, 85867-970 Foz do Iguaçu, PR (Brazil); Holanda, J.; Azevedo, A.; Rezende, S. M., E-mail: [Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE (Brazil); Vilela-Leão, L. H. [Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE (Brazil); Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, 55002-970 Caruaru, PE (Brazil); Rodríguez-Suárez, R. L. [Facultad de Física, Pontificia Universidad Católica de Chile, Casilla 306, Santiago (Chile)


    We report an investigation of the dynamics of the three-magnon splitting process associated with the ferromagnetic resonance (FMR) in films of the insulating ferrimagnet yttrium iron garnet (YIG). The experiments are performed with a 6 μm thick YIG film close to a microstrip line fed by a microwave generator operating in the 2–6 GHz range. The magnetization precession is driven by the microwave rf magnetic field perpendicular to the static magnetic field, and its dynamics is observed by monitoring the amplitude of the FMR absorption peak. The time evolution of the amplitude reveals that if the frequency is lowered below a critical value of 3.3 GHz, the FMR mode pumps two magnons with opposite wave vectors that react back on the FMR, resulting in a nonlinear dynamics of the magnetization. The results are explained by a model with coupled nonlinear equations describing the time evolution of the magnon modes.

  6. Three-dimensional multispecies nonlinear perturbative particle simulations of collective processes in intense particle beams

    Directory of Open Access Journals (Sweden)

    Hong Qin


    Full Text Available Collective processes in intense charged particle beams described self-consistently by the Vlasov-Maxwell equations are studied using a 3D multispecies nonlinear perturbative particle simulation method. The newly developed beam equilibrium, stability, and transport (BEST code is used to simulate the nonlinear stability properties of intense beam propagation, surface eigenmodes in a high-intensity beam, and the electron-proton (e-p two-stream instability observed in the Proton Storage Ring (PSR experiment. Detailed simulations in a parameter regime characteristic of the PSR experiment show that the dipole-mode two-stream instability is stabilized by a modest spread (about 0.1% in axial momentum of the beam particles.

  7. Numerical simulation of nonlinear processes in a beam-plasma system

    Energy Technology Data Exchange (ETDEWEB)

    Efimova, A. A., E-mail:; Berendeev, E. A.; Vshivkov, V. A. [Institute of Computational Mathematics and Mathematical Geophysics SB RAS 6 Acad. Lavrentyev Ave., Novosibirsk 630090 (Russian Federation); Dudnikova, G. I. [University of Maryland, College Park, MD 20742 (United States); Institute of Computational Technologies SB RAS, 6 Acad. Lavrentyev Ave., Novosibirsk 630090 (Russian Federation)


    In the present paper we consider the efficiency of the electromagnetic radiation generation due to various nonlinear processes in the beam-plasma system. The beam and plasma parameters were chosen close to the parameters in the experiment on the GOL-3 facility (BINP SB RAS). The model of the collisionless plasma is described by system of the Vlasov-Maxwell equations with periodic boundary conditions. The parallel numerical algorithm is based on the particles-in-cell method (PIC) with mixed Euler-Lagrangian domain decomposition. Various scenarios of nonlinear evolution in the beam-plasma system under the influence of an external magnetic field in case of a low density beam were studied. The energy transfer from one unstable mode to the others modes was observed.

  8. Linear and Nonlinear Impairment Compensation in Coherent Optical Transmission with Digital Signal Processing

    DEFF Research Database (Denmark)

    Porto da Silva, Edson

    Digital signal processing (DSP) has become one of the main enabling technologies for the physical layer of coherent optical communication networks. The DSP subsystems are used to implement several functionalities in the digital domain, from synchronization to channel equalization. Flexibility...... nonlinearity compensation, (II) spectral shaping, and (III) adaptive equalization. For (I), original contributions are presented to the study of the nonlinearity compensation (NLC) with digital backpropagation (DBP). Numerical and experimental performance investigations are shown for different application...... scenarios. Concerning (II), it is demonstrated how optical and electrical (digital) pulse shaping can be allied to improve the spectral confinement of a particular class of optical time-division multiplexing (OTDM) signals that can be used as a building block for fast signaling single-carrier transceivers...

  9. Anticipation and the Non-linear Dynamics of Meaning-Processing in Social Systems

    CERN Document Server

    Leydesdorff, Loet


    Social order does not exist as a stable phenomenon, but can be considered as "an order of reproduced expectations." When anticipations operate upon one another, they can generate a non-linear dynamics which processes meaning. Although specific meanings can be stabilized, for example in social institutions, all meaning arises from a global horizon of possible meanings. Using Luhmann's (1984) social systems theory and Rosen's (1985) theory of anticipatory systems, I submit algorithms for modeling the non-linear dynamics of meaning in social systems. First, a self-referential system can use a model of itself for the anticipation. Under the condition of functional differentiation, the social system can be expected to entertain a set of models; each model can also contain a model of the other models. Two anticipatory mechanisms are then possible: a transversal one between the models, and a longitudinal one providing the system with a variety of meanings. A system containing two anticipatory mechanisms can become h...

  10. High-speed nonlinear finite element analysis for surgical simulation using graphics processing units. (United States)

    Taylor, Z A; Cheng, M; Ourselin, S


    The use of biomechanical modelling, especially in conjunction with finite element analysis, has become common in many areas of medical image analysis and surgical simulation. Clinical employment of such techniques is hindered by conflicting requirements for high fidelity in the modelling approach, and fast solution speeds. We report the development of techniques for high-speed nonlinear finite element analysis for surgical simulation. We use a fully nonlinear total Lagrangian explicit finite element formulation which offers significant computational advantages for soft tissue simulation. However, the key contribution of the work is the presentation of a fast graphics processing unit (GPU) solution scheme for the finite element equations. To the best of our knowledge, this represents the first GPU implementation of a nonlinear finite element solver. We show that the present explicit finite element scheme is well suited to solution via highly parallel graphics hardware, and that even a midrange GPU allows significant solution speed gains (up to 16.8 x) compared with equivalent CPU implementations. For the models tested the scheme allows real-time solution of models with up to 16,000 tetrahedral elements. The use of GPUs for such purposes offers a cost-effective high-performance alternative to expensive multi-CPU machines, and may have important applications in medical image analysis and surgical simulation.

  11. Effect of alpha and Gaussian refractive index profiles on the design of highly nonlinear optical fibre for efficient nonlinear optical signal processing (United States)

    Selvendran, S.; Sivanantharaja, A.; Arivazhagan, S.; Kannan, M.


    We propose an index profiled, highly nonlinear ultraflattened dispersion fibre (HN-UFF) with appreciable values of fibre parameters such as dispersion, dispersion slope, effective area, nonlinearity, bending loss and splice loss. The designed fibre has normal zero flattened dispersion over S, C, L, U bands and extends up to 1.9857 μm. The maximum dispersion variation observed for this fibre is as low as 1.61 ps km-1 nm-1 over the 500-nm optical fibre transmission spectrum. This fibre also has two zero dispersion wavelengths at 1.487 and 1.9857 μm and the respective dispersion slopes are 0.02476 and 0.0068 ps nm-2 km-1. The fibre has a very low ITU-T cutoff wavelength of 1.2613 μm and a virtuous nonlinear coefficient of 9.43 W-1 km-1. The wide spectrum of zero flattened dispersion and a good nonlinear coefficient make the designed fibre very promising for different nonlinear optical signal processing applications.

  12. Receptor-mediated endocytosis of macromolecules and strategy to enhance their transport in alveolar epithelial cells. (United States)

    Takano, Mikihisa; Kawami, Masashi; Aoki, Ayako; Yumoto, Ryoko


    Pulmonary delivery is an attractive administration route for therapeutic proteins and peptides. In this context, endocytosis/transcytosis at the distal lung epithelial barrier is an important process in the pulmonary absorption of therapeutic macromolecules. The alveolar epithelium is comprised of type I and type II cells. Understanding the transport mechanisms in these cells is essential for the development of efficient pulmonary delivery systems of therapeutic macromolecules. Endocytic pathways for albumin and insulin in alveolar epithelial cells and possible receptors for the endocytosis are discussed. Strategies to enhance the endocytosis and pulmonary absorption of macromolecules are also discussed, by focusing on the effects of cationic poly(amino acid)s. Although the surface area occupied by type II cells in alveoli is much smaller than that covered by type I cells, type II cells may significantly contribute to the endocytosis/transcytosis of macromolecules such as albumin. Identification of the receptors involved in the cellular uptake of each macromolecule is prerequisite for the understanding and regulation of its transport into and across alveolar epithelial cells. Establishment of novel in-vitro culture cell models of type I and type II cells would be a great help for the future advance of this research field.

  13. Mechanoporation of living cells for delivery of macromolecules using nanoneedle array. (United States)

    Matsumoto, Daisuke; Yamagishi, Ayana; Saito, Megumi; Sathuluri, Ramachandra Rao; Silberberg, Yaron R; Iwata, Futoshi; Kobayashi, Takeshi; Nakamura, Chikashi


    Efficient and rapid delivery of macromolecule probes, such as quenchbodies and other large biomarkers that cannot readily pass through the plasma membrane, is necessary for live-cell imaging and other intracellular analyses. We present here an alternative, simple method for delivery of macromolecules into live cells. In this method, which we term here mechanoporation, a nanoneedle array is used for making transient pores in the plasma membrane to allow access of desired macromolecules into thousands of live cells, simultaneously. This rapid, 3-step method facilitates an efficient delivery by adding macromolecules into the medium, inserting nanoneedles into the cells and oscillating the nanoneedle array, a process that takes no more than 5 min in total. In addition, we demonstrate here how this method can repeatedly and reproducibly deliver molecules into specifically-selected locations on a given cell culture dish. The results presented here show how this unique mechanoporation method enables rapid and high-throughput bio-macromolecule delivery and live-cell imaging. Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  14. Non-equilibrium condensation process in holographic superconductor with nonlinear electrodynamics

    CERN Document Server

    Liu, Yunqi; Wang, Bin


    We study the non-equilibrium condensation process in a holographic superconductor with nonlinear corrections to the U(1) gauge field. We start with an asymptotic Anti-de-Sitter(AdS) black hole against a complex scalar perturbation at the initial time, and solve the dynamics of the gravitational systems in the bulk. When the black hole temperature T is smaller than a critical value Tc, the scalar perturbation grows exponentially till saturation, the final state of spacetime approaches to a hairy black hole. In the bulk theory, we find the clue of the influence of nonlinear corrections in the gauge field on the process of the scalar field condensation. We show that the bulk dynamics in the non-equilibrium process is completely consistent with the observations on the boundary order parameter. Furthermore we examine the time evolution of horizons in the bulk non-equilibrium transformation process from the bald AdS black hole to the AdS hairy hole. Both the evolution of apparent and event horizons show that the or...

  15. Generation of photon pairs through parametric processes in nonlinear waveguides with the account of losses (United States)

    Vavulin, D. N.; Sukhorukov, A. A.


    We present an analytical description of the process of spontaneous four-wave mixing in a cubic nonlinear fiber with linear losses. We consider the generation of photon pairs in the fiber when in the input of fiber is fed the pumping wave and single signal photon. The focus of attention is on three cases: when the signal photon propagates in the fiber without generating of biphotons; when the photon pair is generated; and when the photon is lost in the fiber. We also consider the cascade processes, but do not give them an analytical description because of their smallness. Description of the biphotons generation process we provide using the Schrodinger-type equation, and take into account the losses in the fiber through the introduction of the virtual beam splitters. We demonstrate the effectiveness of the generation of photon pairs through parametric processes.

  16. Definition of distance for nonlinear time series analysis of marked point process data

    Energy Technology Data Exchange (ETDEWEB)

    Iwayama, Koji, E-mail: [Research Institute for Food and Agriculture, Ryukoku Univeristy, 1-5 Yokotani, Seta Oe-cho, Otsu-Shi, Shiga 520-2194 (Japan); Hirata, Yoshito; Aihara, Kazuyuki [Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 (Japan)


    Marked point process data are time series of discrete events accompanied with some values, such as economic trades, earthquakes, and lightnings. A distance for marked point process data allows us to apply nonlinear time series analysis to such data. We propose a distance for marked point process data which can be calculated much faster than the existing distance when the number of marks is small. Furthermore, under some assumptions, the Kullback–Leibler divergences between posterior distributions for neighbors defined by this distance are small. We performed some numerical simulations showing that analysis based on the proposed distance is effective. - Highlights: • A new distance for marked point process data is proposed. • The distance can be computed fast enough for a small number of marks. • The method to optimize parameter values of the distance is also proposed. • Numerical simulations indicate that the analysis based on the distance is effective.

  17. A Stochastic Finite Element Model for the Dynamics of Globular Macromolecules

    CERN Document Server

    Oliver, Robin; Harlen, Oliver G; Harris, Sarah A


    We describe a novel coarse-grained simulation method for modelling the dynamics of globular macromolecules, such as proteins. The macromolecule is treated as a continuum that is subject to thermal fluctuations. The model includes a non-linear treatment of elasticity and viscosity with thermal noise that is solved using finite element analysis. We have validated the method by demonstrating that the model provides average kinetic and potential energies that are in agreement with the classical equipartition theorem. In addition, we have performed Fourier analysis on the simulation trajectories obtained for a series of linear beams to confirm that the correct average energies are present in the first two Fourier bending modes. We have then used the new modelling method to simulate the thermal fluctuations of a representative protein over 500ns timescales. Using reasonable parameters for the material properties, we have demonstrated that the overall deformation of the biomolecule is consistent with the results obt...

  18. Intrinsic Nonlinearities and Layout Impacts of 100 V Integrated Power MOSFETs in Partial SOI Process

    DEFF Research Database (Denmark)

    Fan, Lin; Knott, Arnold; Jørgensen, Ivan Harald Holger

    Parasitic capacitances of power semiconductors are a part of the key design parameters of state-of-the-art very high frequency (VHF) power supplies. In this poster, four 100 V integrated power MOSFETs with different layout structures are designed, implemented, and analyzed in a 0.18 ȝm partial...... Silicon-on-Insulator (SOI) process with a die area 2.31 mm2.  A small-signal model of power MOSFETs is proposed to systematically analyze the nonlinear parasitic capacitances in different transistor states: off-state, sub-threshold region, and on-state in the linear region. 3D plots are used to summarize...

  19. Time-ordering effects in the generation of entangled photons using nonlinear optical processes. (United States)

    Quesada, Nicolás; Sipe, J E


    We study the effects of time ordering in photon generation processes such as spontaneous parametric down-conversion (SPDC) and four wave mixing (SFWM). The results presented here are used to construct an intuitive picture that allows us to predict when time-ordering effects significantly modify the joint spectral amplitude (JSA) of the photons generated in SPDC and SFWM. These effects become important only when the photons being generated lie with the pump beam that travels through the nonlinear material for a significant amount of time. Thus sources of spectrally separable photons are ideal candidates for the observation of modifications of the JSA due to time ordering.

  20. Imitation learning of Non-Linear Point-to-Point Robot Motions using Dirichlet Processes

    DEFF Research Database (Denmark)

    Krüger, Volker; Tikhanoff, Vadim; Natale, Lorenzo


    In this paper we discuss the use of the infinite Gaussian mixture model and Dirichlet processes for learning robot movements from demonstrations. Starting point of this work is an earlier paper where the authors learn a non-linear dynamic robot movement model from a small number of observations....... The model in that work is learned using a classical finite Gaussian mixture model (FGMM) where the Gaussian mixtures are appropriately constrained. The problem with this approach is that one needs to make a good guess for how many mixtures the FGMM should use. In this work, we generalize this approach...

  1. ADI splitting schemes for a fourth-order nonlinear partial differential equation from image processing

    KAUST Repository

    Calatroni, Luca


    We present directional operator splitting schemes for the numerical solution of a fourth-order, nonlinear partial differential evolution equation which arises in image processing. This equation constitutes the H -1-gradient flow of the total variation and represents a prototype of higher-order equations of similar type which are popular in imaging for denoising, deblurring and inpainting problems. The efficient numerical solution of this equation is very challenging due to the stiffness of most numerical schemes. We show that the combination of directional splitting schemes with implicit time-stepping provides a stable and computationally cheap numerical realisation of the equation.

  2. A process fault estimation strategy for non-linear dynamic systems (United States)

    Pazera, Marcin; Korbicz, Józef


    The paper deals with the problem of simultaneous state and process fault estimation for non-linear dynamic systems. Instead of estimating the fault directly, its product with state and the state itself are estimated. To derive the fault from the product, a simple algebraic approach is proposed. The estimation strategy is based on the quadratic boundedness approach. The final part of the paper presents an illustrative example concerning a laboratory multi-tank system. The real data experiments clearly exhibit the performance of the proposed approach.

  3. Terahertz spectroscopy of dry, hydrated, and thermally denatured biological macromolecules (United States)

    Lipscomb, Dawn; Echchgadda, Ibtissam; Ibey, Bennett L.; Beier, Hope; Thomas, Robert J.; Peralta, Xomalin; Wilmink, Gerald J.


    Terahertz time-domain spectroscopy (THz-TDS) is an effective technique to probe the intermolecular and collective vibrational modes of biological macromolecules at THz frequencies. To date, the vast majority of spectroscopic studies have been performed on dehydrated biomolecular samples. Given the fact that all biochemical processes occur in aqueous environments and water is required for proper protein folding and function, we hypothesize that valuable information can be gained from spectroscopic studies performed on hydrated biomolecules in their native conformation. In this study, we used a THz-TDS system that exploits photoconductive techniques for THz pulse generation and freespace electro-optical sampling approaches for detection. We used the THz spectrometer to measure the time-dependent electric field of THz waves upon interaction with water, phosphate buffered saline (PBS), and collagen gels. By comparing these waveforms with references, we simultaneously determined each sample's index of refraction (n) and absorption coefficients (μa) as a function of frequency. Our data show that the properties we measure for the water, PBS and collagen are comparable to those reported in the literature. In the future, we plan to examine the effect that both temperature and pH have on the optical properties of other biological macromolecules. Studies will also be performed to compare our results to those generated using molecular dynamics simulations.

  4. Estimation and filtering of nonlinear systems application to a waste-water treatment process

    Energy Technology Data Exchange (ETDEWEB)

    Ben Youssef, C.; Dahhou, B. [Centre National de la Recherche Scientifique (CNRS), 31 - Toulouse (France). Lab. d`Automatique et d`Analyse des Systemes]|[Institut National des Sciences Appliquees (INSA), 31 - Toulouse (France); Zeng, F.Y.; Rols, J.L. [Institut National des Sciences Appliquees (INSA), 31 - Toulouse (France)


    A fundamental task in design and control of biotechnological processes is system modelling. This task is made difficult by the scarceness of on-line direct sensors for some key variables and by the fact that identifiability of models including Michaelis-Menten type of nonlinearities is not straightforward. The use of adaptive estimation approaches constitutes an interesting alternative to circumvent these kind of problems. This paper discusses an identification technique derived to solve the problem of estimating simultaneously inaccessible state variables and time-varying parameters of a nonlinear wastewater treatment process. An extended linearization technique using Kronecker`s calculation provides the error model of the joint observer-estimator procedure which convergence is proved via Lyapunov`s method. Sufficient conditions for stability of this joint identification scheme are given and discussed according to the persistence excitation conditions of the signals. A simulation study with measurement noises and abrupt jumps of the process parameters shows the feasibility and significant robustness of the proposed adaptive estimation methodologies. (author). (author). 10 refs., 3 figs.

  5. Templated and self-limiting calcite formation directed by coccolith organic macromolecules. (United States)

    Gal, Assaf; Wirth, Richard; Barkay, Zahava; Eliaz, Noam; Scheffel, André; Faivre, Damien


    The formation of intricately shaped crystalline minerals by organisms is orchestrated by specialized biomacromolecules. The macromolecules associated with coccoliths, nanometer-sized calcite crystal arrays produced by marine microalgae, can form a distinct calcium-rich phase via macromolecular recognition. Here, we show that this calcium-rich phase can be mineralized into a thin film of single-crystalline calcite by the balanced addition of carbonate ions. Such a crystallization process provides a strategy to direct crystalline products via local interactions between soluble macromolecules and compatible templates.

  6. A new cellular nonlinear network emulation on FPGA for EEG signal processing in epilepsy (United States)

    Müller, Jens; Müller, Jan; Tetzlaff, Ronald


    For processing of EEG signals, we propose a new architecture for the hardware emulation of discrete-time Cellular Nonlinear Networks (DT-CNN). Our results show the importance of a high computational accuracy in EEG signal prediction that cannot be achieved with existing analogue VLSI circuits. The refined architecture of the processing elements and its resource schedule, the cellular network structure with local couplings, the FPGA-based embedded system containing the DT-CNN, and the data flow in the entire system will be discussed in detail. The proposed DT-CNN design has been implemented and tested on an Xilinx FPGA development platform. The embedded co-processor with a multi-threading kernel is utilised for control and pre-processing tasks and data exchange to the host via Ethernet. The performance of the implemented DT-CNN has been determined for a popular example and compared to that of a conventional computer.

  7. Global analysis of fluorescence decays to probe the internal dynamics of fluorescently labeled macromolecules. (United States)

    Duhamel, Jean


    The aim of this review is to introduce the reader first to the mathematical complexity associated with the analysis of fluorescence decays acquired with solutions of macromolecules labeled with a fluorophore and its quencher that are capable of interacting with each other via photophysical processes within the macromolecular volume, second to the experimental and mathematical approaches that have been proposed over the years to handle this mathematical complexity, and third to the information that one can expect to retrieve with respect to the internal dynamics of such fluorescently labeled macromolecules. In my view, the ideal fluorophore-quencher pair to use in studying the internal dynamics of fluorescently labeled macromolecules would involve a long-lived fluorophore, a fluorophore and a quencher that do not undergo energy migration, and a photophysical process that results in a change in fluorophore emission upon contact between the excited fluorophore and quencher. Pyrene, with its ability to form an excimer on contact between excited-state and ground-state species, happens to possess all of these properties. Although the concepts described in this review apply to any fluorophore and quencher pair sharing pyrene's exceptional photophysical properties, this review focuses on the study of pyrene-labeled macromolecules that have been characterized in great detail over the past 40 years and presents the main models that are being used today to analyze the fluorescence decays of pyrene-labeled macromolecules reliably. These models are based on Birks' scheme, the DMD model, the fluorescence blob model, and the model free analysis. The review also provides a step-by-step protocol that should enable the noneducated user to achieve a successful decay analysis exempt of artifacts. Finally, some examples of studies of pyrene-labeled macromolecules are also presented to illustrate the different types of information that can be retrieved from these fluorescence decay

  8. Nonlinear software sensor for monitoring genetic regulation processes with noise and modeling errors (United States)

    Ibarra-Junquera, V.; Torres, L. A.; Rosu, H. C.; Argüello, G.; Collado-Vides, J.


    Nonlinear control techniques by means of a software sensor that are commonly used in chemical engineering could be also applied to genetic regulation processes. We provide here a realistic formulation of this procedure by introducing an additive white Gaussian noise, which is usually found in experimental data. Besides, we include model errors, meaning that we assume we do not know the nonlinear regulation function of the process. In order to illustrate this procedure, we employ the Goodwin dynamics of the concentrations [B. C. Goodwin, Temporal Oscillations in Cells (Academic, New York, 1963)] in the simple form recently applied to single gene systems and some operon cases [H. De Jong, J. Comput. Biol. 9, 67 (2002)], which involves the dynamics of the mRNA, given protein and metabolite concentrations. Further, we present results for a three gene case in coregulated sets of transcription units as they occur in prokaryotes. However, instead of considering their full dynamics, we use only the data of the metabolites and a designed software sensor. We also show, more generally, that it is possible to rebuild the complete set of nonmeasured concentrations despite the uncertainties in the regulation function or, even more, in the case of not knowing the mRNA dynamics. In addition, the rebuilding of concentrations is not affected by the perturbation due to the additive white Gaussian noise and also we managed to filter the noisy output of the biological system.

  9. Sub-diffraction imaging on standard microscopes through photobleaching microscopy with non-linear processing. (United States)

    Munck, Sebastian; Miskiewicz, Katarzyna; Sannerud, Ragna; Menchon, Silvia A; Jose, Liya; Heintzmann, Rainer; Verstreken, Patrik; Annaert, Wim


    Visualization of organelles and molecules at nanometer resolution is revolutionizing the biological sciences. However, such technology is still limited for many cell biologists. We present here a novel approach using photobleaching microscopy with non-linear processing (PiMP) for sub-diffraction imaging. Bleaching of fluorophores both within the single-molecule regime and beyond allows visualization of stochastic representations of sub-populations of fluorophores by imaging the same region over time. Our method is based on enhancing the probable positions of the fluorophores underlying the images. The random nature of the bleached fluorophores is assessed by calculating the deviation of the local actual bleached fluorescence intensity to the average bleach expectation as given by the overall decay of intensity. Subtracting measured from estimated decay images yields differential images. Non-linear enhancement of maxima in these diffraction-limited differential images approximates the positions of the underlying structure. Summing many such processed differential images yields a super-resolution PiMP image. PiMP allows multi-color, three-dimensional sub-diffraction imaging of cells and tissues using common fluorophores and can be implemented on standard wide-field or confocal systems.

  10. Preface: Special Topic on Coarse Graining of Macromolecules, Biopolymers, and Membranes. (United States)

    Holm, Christian; Gompper, Gerhard; Dill, Ken A


    This special issue highlights new developments in theory and coarse-graining in biological and synthetic macromolecules and membranes. Such approaches give unique insights into the principles and design of the structures, dynamics, and assembly processes of these complex fluids and soft materials, where the length and time scales are often prohibitively long for fully atomistic modeling.

  11. Preface: Special Topic on Coarse Graining of Macromolecules, Biopolymers, and Membranes

    Energy Technology Data Exchange (ETDEWEB)

    Holm, Christian [Institut für Computerphysik, Universität Stuttgart, Allmandring 3, 70569 Stuttgart (Germany); Gompper, Gerhard [Theoretical Soft Matter and Biophysics, Institute of Complex Systems and Institute for Advanced Simulation, Forschungszentrum Jülich, 52428 Jülich (Germany); Dill, Ken A. [Department of Chemistry, Stony Brook University, Stony Brook, New York 11794 (United States)


    This special issue highlights new developments in theory and coarse-graining in biological and synthetic macromolecules and membranes. Such approaches give unique insights into the principles and design of the structures, dynamics, and assembly processes of these complex fluids and soft materials, where the length and time scales are often prohibitively long for fully atomistic modeling.

  12. Hybrid Opto-electric Manipulation of Macromolecules (United States)

    Wereley, Steve


    Recently our research group has developed an innovative method for capturing, concentrating, manipulating and sorting populations of micro- and nanometer-scaled entities-particles, cells, macro-molecules, etc. These populations range from individual particles to thousands of particles (Lab-on-a-Chip, 2008; Microfluidics and Nanofluidics, 2008) while the sizes range from microns to nanometers. This novel technique combines features of optical trapping and dielectrophoresis in an innovative, dynamic way using a simple parallel plate electrode configuration. Transparent electrodes comprised of Indium Tin Oxide (ITO) on glass substrates are used to generate an electric field in the fluid while at the same time allowing light into and out of the fluid. Near-IR optical illumination causes subtle localized heating, creating an electric permittivity gradient that in turn drives a microscopic toroidal vortex. The vortex efficiently transports particles to a preferred location, usually the surface of the electrode. Recent advances have extended have allowed us to apply this technique to macromolecules (DNA, proteins) as well as nanoscale particles (quantum dots, nanowires and PSL particles).

  13. Nonlinear modeling and dynamic analysis of a hydro-turbine governing system in the process of sudden load increase transient (United States)

    Li, Huanhuan; Chen, Diyi; Zhang, Hao; Wang, Feifei; Ba, Duoduo


    In order to study the nonlinear dynamic behaviors of a hydro-turbine governing system in the process of sudden load increase transient, we establish a novel nonlinear dynamic model of the hydro-turbine governing system which considers the elastic water-hammer model of the penstock and the second-order model of the generator. The six nonlinear dynamic transfer coefficients of the hydro-turbine are innovatively proposed by utilizing internal characteristics and analyzing the change laws of the characteristic parameters of the hydro-turbine governing system. Moreover, from the point of view of engineering, the nonlinear dynamic behaviors of the above system are exhaustively investigated based on bifurcation diagrams and time waveforms. More importantly, all of the above analyses supply theoretical basis for allowing a hydropower station to maintain a stable operation in the process of sudden load increase transient.

  14. Pole-placement self-tuning control of nonlinear Hammerstein system and its application to pH process control

    Institute of Scientific and Technical Information of China (English)

    Zhiyun Zou; Dandan Zhao; Xinghong Liu; Yuqing Guo; Chen Guan; Wenqiang Feng; Ning Guo


    By taking advantage of the separation characteristics of nonlinear gain and dynamic sector inside a Hammerstein model, a novel pole placement self tuning control scheme for nonlinear Hammerstein system was put forward based on the linear system pole placement self tuning control algorithm. And the nonlinear Hammerstein system pole placement self tuning control (NL-PP-STC) algorithm was presented in detail. The identification ability of its parameter estimation algorithm of NL-PP-STC was analyzed, which was always identifiable in closed loop. Two particular problems including the selection of poles and the on-line estimation of model parameters, which may be met in applications of NL-PP-STC to real process control, were discussed. The control simulation of a strong nonlinear pH neutralization process was carried out and good control performance was achieved.

  15. Separating iterative solution model of generalized nonlinear dynamic least squares for data processing in building of digital earth

    Institute of Scientific and Technical Information of China (English)

    陶华学; 郭金运


    Data coming from different sources have different types and temporal states. Relations between one type of data and another ones, or between data and unknown parameters are almost nonlinear. It is not accurate and reliable to process the data in building the digital earth with the classical least squares method or the method of the common nonlinear least squares. So a generalized nonlinear dynamic least squares method was put forward to process data in building the digital earth. A separating solution model and the iterative calculation method were used to solve the generalized nonlinear dynamic least squares problem. In fact, a complex problem can be separated and then solved by converting to two sub-problems, each of which has a single variable. Therefore the dimension of unknown parameters can be reduced to its half, which simplifies the original high dimensional equations.

  16. Sensor fault diagnosis of nonlinear processes based on structured kernel principal component analysis

    Institute of Scientific and Technical Information of China (English)

    Kechang FU; Liankui DAI; Tiejun WU; Ming ZHU


    A new sensor fault diagnosis method based on structured kernel principal component analysis (KPCA) is proposed for nonlinear processes.By performing KPCA on subsets of variables,a set of structured residuals,i.e.,scaled powers of KPCA,can be obtained in the same way as partial PCA.The structured residuals are utilized in composing an isolation scheme for sensor fault diagnosis,according to a properly designed incidence matrix.Sensor fault sensitivity and critical sensitivity are defined,based on which an incidence matrix optimization algorithm is proposed to improve the performance of the structured KPCA.The effectiveness of the proposed method is demonstrated on the simulated continuous stirred tank reactor (CSTR) process.

  17. Development of a robust calibration model for nonlinear in-line process data (United States)

    Despagne; Massart; Chabot


    A comparative study involving a global linear method (partial least squares), a local linear method (locally weighted regression), and a nonlinear method (neural networks) has been performed in order to implement a calibration model on an industrial process. The models were designed to predict the water content in a reactor during a distillation process, using in-line measurements from a near-infrared analyzer. Curved effects due to changes in temperature and variations between the different batches make the problem particularly challenging. The influence of spectral range selection and data preprocessing has been studied. With each calibration method, specific procedures have been applied to promote model robustness. In particular, the use of a monitoring set with neural networks does not always prevent overfitting. Therefore, we developed a model selection criterion based on the determination of the median of monitoring error over replicate trials. The back-propagation neural network models selected were found to outperform the other methods on independent test data.

  18. Nonlinear mechanisms to Rogue events in the process of interaction between optical filaments

    CERN Document Server

    Kovachev, L M


    We investigate two types of nonlinear interaction between collinear femtosecond laser pulses with power slightly above the critical for self-focusing $P_{cr}$. In the first case we study energy exchange between filaments. The model describes this process through degenerate four-photon parametric mixing (FPPM) scheme and requests initial phase difference between the waves. When there are no initial phase difference between the pulses, the FPPM process does not work. In this case it is obtained the second type of interaction as merging between two, three or four filaments in a single filament with higher power. It is found that in the second case the interflow between the filaments has potential of interaction due to cross-phase modulation (CPM).

  19. Improved Kernel PLS-based Fault Detection Approach for Nonlinear Chemical Processes

    Institute of Scientific and Technical Information of China (English)

    王丽; 侍洪波


    In this paper, an improved nonlinear process fault detection method is proposed based on modified ker-nel partial least squares (KPLS). By integrating the statistical local approach (SLA) into the KPLS framework, two new statistics are established to monitor changes in the underlying model. The new modeling strategy can avoid the Gaussian distribution assumption of KPLS. Besides, advantage of the proposed method is that the kernel latent variables can be obtained directly through the eigen value decomposition instead of the iterative calculation, which can improve the computing speed. The new method is applied to fault detection in the simulation benchmark of the Tennessee Eastman process. The simulation results show superiority on detection sensitivity and accuracy in com-parison to KPLS monitoring.

  20. Innovation as a Nonlinear Process, the Scientometric Perspective, and the Specification of an "Innovation Opportunities Explorer"

    CERN Document Server

    Leydesdorff, Loet; de Nooy, Wouter


    The process of innovation follows non-linear patterns across the domains of science, technology, and the economy. Novel bibliometric mapping techniques can be used to investigate and represent distinctive, but complementary perspectives on the innovation process (e.g., "demand" and "supply") as well as the interactions among these perspectives. The perspectives can be represented as "continents" of data related to varying extents over time. For example, the different branches of Medical Subject Headings (MeSH) in the Medline database provide sources of such perspectives (e.g., "Diseases" versus "Drugs and Chemicals"). The multiple-perspective approach enables us to reconstruct facets of the dynamics of innovation, in terms of selection mechanisms shaping localizable trajectories and/or resulting in more globalized regimes. By expanding the data with patents and scholarly publications, we demonstrate the use of this multi-perspective approach in the case of RNA Interference (RNAi). The possibility to develop a...

  1. Automated System of Study Nonlinear Processes in Electro-vacuum Devices with Open Resonant Periodic Structures

    Directory of Open Access Journals (Sweden)

    G.S. Vorobyov


    Full Text Available The article describes the experimental equipment and the results of investigations of nonlinear processes occurring during the excitation of electromagnetic oscillations in the resonant electron beam devices such as an orotron-generator of diffraction radiation. These devices are finding wide application in physics and microwave technology, now. A technique for experimental research, which bases on the using of the universal electro vacuum equipment diffraction radiation analyzer and the microprocessor system for collecting and processing data. The experimental investigations results of the energy and frequency characteristics for the most common modes of the excitation oscillations in the open resonant systems such as an orotron. The implementations on the optimum modes for the oscillations excitation in such devices were recommended.

  2. PlaMoM: a comprehensive database compiles plant mobile macromolecules. (United States)

    Guan, Daogang; Yan, Bin; Thieme, Christoph; Hua, Jingmin; Zhu, Hailong; Boheler, Kenneth R; Zhao, Zhongying; Kragler, Friedrich; Xia, Yiji; Zhang, Shoudong


    In plants, various phloem-mobile macromolecules including noncoding RNAs, mRNAs and proteins are suggested to act as important long-distance signals in regulating crucial physiological and morphological transition processes such as flowering, plant growth and stress responses. Given recent advances in high-throughput sequencing technologies, numerous mobile macromolecules have been identified in diverse plant species from different plant families. However, most of the identified mobile macromolecules are not annotated in current versions of species-specific databases and are only available as non-searchable datasheets. To facilitate study of the mobile signaling macromolecules, we compiled the PlaMoM (Plant Mobile Macromolecules) database, a resource that provides convenient and interactive search tools allowing users to retrieve, to analyze and also to predict mobile RNAs/proteins. Each entry in the PlaMoM contains detailed information such as nucleotide/amino acid sequences, ortholog partners, related experiments, gene functions and literature. For the model plant Arabidopsis thaliana, protein-protein interactions of mobile transcripts are presented as interactive molecular networks. Furthermore, PlaMoM provides a built-in tool to identify potential RNA mobility signals such as tRNA-like structures. The current version of PlaMoM compiles a total of 17 991 mobile macromolecules from 14 plant species/ecotypes from published data and literature. PlaMoM is available at © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  3. Slow and fast light using nonlinear processes in semiconductor optical amplifiers (United States)

    Pesala, Bala Subrahmanyam

    Ability to control the velocity of light is usually referred to as slow or fast light depending on whether the group velocity of light is reduced or increased. The slowing of light as it passes through the glass to 2/3rd its original value is a well known phenomenon. This slowing down happens due to the interaction of light with the electrons in the medium. As a general principle, stronger the interaction, larger is the reduction in velocity. Recently, a fascinating field has emerged with the objective of not only slowing down the velocity of light but also speeding it up as it goes through the medium by enhancing light-matter interaction. This unprecedented control opens up several exciting applications in various scientific disciplines ranging from nonlinear science, RF photonics to all-optical networks. Initial experiments succeeded in reducing the velocity of light more than a million times to a very impressive 17 m/s. This speed reduction is extremely useful to enhance various nonlinear processes. For RF photonic applications including phased array antennas and tunable filters, control of phase velocity of light is required while control of group velocity serves various functionalities including packet synchronization and contention resolution in an optical buffer. Within the last 10 years, several material systems have been proposed and investigated for this purpose. Schemes based on semiconductor systems for achieving slow and fast light has the advantage of extremely high speed and electrical control. In addition, they are compact, operate at room temperature and can be easily integrated with other optical subsystems. In this work, we propose to use nonlinear processes in semiconductor optical amplifiers (SOAs) for the purpose of controlling the velocity of light. The versatility of the physical processes present in SOAs enables the control of optical signals ranging from 1GHz to larger than 1000 GHz (1 THz). First, we experimentally demonstrate both

  4. Nonlinear optical signal processing for high-speed, spectrally efficient fiber optic systems and networks (United States)

    Zhang, Bo

    The past decade has witnessed astounding boom in telecommunication network traffic. With the emergence of multimedia over Internet, the high-capacity optical transport systems have started to shift focus from the core network towards the end users. This trend leads to diverse optical networks with transparency and reconfigurability requirement. As single channel data rate continues to increase and channel spacing continues to shrink for high capacity, high spectral efficiency, the workload on conventional electronic signal processing elements in the router nodes continues to build up. Performing signal processing functions in the optical domain can potentially alleviate the speed bottleneck if the unique optical properties are efficiently leveraged to assist electronic processing methodologies. Ultra-high bandwidth capability along with the promise for multi-channel and format-transparent operation make optical signal processing an attractive technology which is expected to have great impact on future optical networks. For optical signal processing applications in fiber-optic network and systems, a laudable goal would be to explore the unique nonlinear optical processes in novel photonic devices. This dissertation investigates novel optical signal processing techniques through simulations and experimental demonstrations, analyzes limitations of these nonlinear processing elements and proposes techniques to enhance the system performance or designs for functional photonic modules. Two key signal-processing building blocks for future optical networks, namely slow-light-based tunable optical delay lines and SOA-based high-speed wavelength converters, are presented in the first part of the dissertation. Phase preserving and spectrally efficient slow light are experimentally demonstrated using advanced modulation formats. Functional and novel photonic modules, such as multi-channel synchronizer and variable-bit-rate optical time division multiplexer are designed and

  5. Accelerated Degradation Process Analysis Based on the Nonlinear Wiener Process with Covariates and Random Effects

    Directory of Open Access Journals (Sweden)

    Li Sun


    Full Text Available It is assumed that the drift parameter is dependent on the acceleration variables and the diffusion coefficient remains the same across the whole accelerated degradation test (ADT in most of the literature based on Wiener process. However, the diffusion coefficient variation would also become obvious in some applications with the stress increasing. Aiming at the phenomenon, the paper concludes that both the drift parameter and the diffusion parameter depend on stress variables based on the invariance principle of failure mechanism and Nelson assumption. Accordingly, constant stress accelerated degradation process (CSADP and step stress accelerated degradation process (SSADP with random effects are modeled. The unknown parameters in the established model are estimated based on the property of degradation and degradation increment, separately for CASDT and SSADT, by the maximum likelihood estimation approach with measurement error. In addition, the simulation steps of accelerated degradation data are provided and simulated step stress accelerated degradation data is designed to validate the proposed model compared to other models. Finally, a case study of CSADT is conducted to demonstrate the benefits of our model in the practical engineering.

  6. Phonon-assisted nonlinear optical processes in ultrashort-pulse pumped optical parametric amplifiers (United States)

    Isaienko, Oleksandr; Robel, István


    Optically active phonon modes in ferroelectrics such as potassium titanyl phosphate (KTP) and potassium titanyl arsenate (KTA) in the ~7-20 THz range play an important role in applications of these materials in Raman lasing and terahertz wave generation. Previous studies with picosecond pulse excitation demonstrated that the interaction of pump pulses with phonons can lead to efficient stimulated Raman scattering (SRS) accompanying optical parametric oscillation or amplification processes (OPO/OPA), and to efficient polariton-phonon scattering. In this work, we investigate the behavior of infrared OPAs employing KTP or KTA crystals when pumped with ~800-nm ultrashort pulses of duration comparable to the oscillation period of the optical phonons. We demonstrate that under conditions of coherent impulsive Raman excitation of the phonons, when the effective χ(2) nonlinearity cannot be considered instantaneous, the parametrically amplified waves (most notably, signal) undergo significant spectral modulations leading to an overall redshift of the OPA output. The pump intensity dependence of the redshifted OPA output, the temporal evolution of the parametric gain, as well as the pump spectral modulations suggest the presence of coupling between the nonlinear optical polarizations PNL of the impulsively excited phonons and those of parametrically amplified waves.

  7. Tracking instantaneous entropy in heartbeat dynamics through inhomogeneous point-process nonlinear models. (United States)

    Valenza, Gaetano; Citi, Luca; Scilingo, Enzo Pasquale; Barbieri, Riccardo


    Measures of entropy have been proved as powerful quantifiers of complex nonlinear systems, particularly when applied to stochastic series of heartbeat dynamics. Despite the remarkable achievements obtained through standard definitions of approximate and sample entropy, a time-varying definition of entropy characterizing the physiological dynamics at each moment in time is still missing. To this extent, we propose two novel measures of entropy based on the inho-mogeneous point-process theory. The RR interval series is modeled through probability density functions (pdfs) which characterize and predict the time until the next event occurs as a function of the past history. Laguerre expansions of the Wiener-Volterra autoregressive terms account for the long-term nonlinear information. As the proposed measures of entropy are instantaneously defined through such probability functions, the proposed indices are able to provide instantaneous tracking of autonomic nervous system complexity. Of note, the distance between the time-varying phase-space vectors is calculated through the Kolmogorov-Smirnov distance of two pdfs. Experimental results, obtained from the analysis of RR interval series extracted from ten healthy subjects during stand-up tasks, suggest that the proposed entropy indices provide instantaneous tracking of the heartbeat complexity, also allowing for the definition of complexity variability indices.

  8. Nonlinear supratransmission

    Energy Technology Data Exchange (ETDEWEB)

    Geniet, F; Leon, J [Physique Mathematique et Theorique, CNRS-UMR 5825, 34095 Montpellier (France)


    A nonlinear system possessing a natural forbidden band gap can transmit energy of a signal with a frequency in the gap, as recently shown for a nonlinear chain of coupled pendulums (Geniet and Leon 2002 Phys. Rev. Lett. 89 134102). This process of nonlinear supratransmission, occurring at a threshold that is exactly predictable in many cases, is shown to have a simple experimental realization with a mechanical chain of pendulums coupled by a coil spring. It is then analysed in more detail. First we go to different (nonintegrable) systems which do sustain nonlinear supratransmission. Then a Josephson transmission line (a one-dimensional array of short Josephson junctions coupled through superconducting wires) is shown to also sustain nonlinear supratransmission, though being related to a different class of boundary conditions, and despite the presence of damping, finiteness, and discreteness. Finally, the mechanism at the origin of nonlinear supratransmission is found to be a nonlinear instability, and this is briefly discussed here.

  9. Low Doses of Traditional Nanophytomedicines for Clinical Treatment: Manufacturing Processes and Nonlinear Response Patterns. (United States)

    Bell, Iris R; Sarter, Barbara; Standish, Leanna J; Banerji, Prasanta; Banerji, Pratip


    The purpose of the present paper is to (a) summarize evidence for the nanoparticle nature and biological effects of traditional homeopathically-prepared medicines at low and ultralow doses; (b) provide details of historically-based homeopathic green manufacturing materials and methods, relating them to top-down mechanical attrition and plant-based biosynthetic processes in modern nanotechnology; (c) outline the potential roles of nonlinear dose-responses and dynamical interactions with complex adaptive systems in generating endogenous amplification processes during low dose treatment. Possible mechanisms of low dose effects, for which there is evidence involving nanoparticles and/or homeopathically-manufactured medicines, include hormesis, time-dependent sensitization, and stochastic resonance. All of the proposed mechanisms depend upon endogenous nonlinear amplification processes in the recipient organism in interaction with the salient, albeit weak signal properties of the medicine. Conventional ligand-receptor mechanisms relevant to higher doses are less likely involved. Effects, especially for homeopathically-prepared nanophytomedicines, include bidirectional host state-dependent changes in function. Homeopathic clinicians report successful treatment of serious infections and cancers. Preclinical biological evidence is consistent with such claims. Controlled biological data on homeopathically-prepared medicines indicate modulation of gene expression and biological signaling pathways regulating cell cycles, immune reactions, and central nervous system function from studies on cells, animals, and human subjects. As a 200-year old system of traditional medicine used by millions of people worldwide, homeopathy offers a pulsed low dose treatment strategy and strong safety record to facilitate progress in translational nanomedicine with plants and other natural products. In turn, modern nanotechnology methods can improve homeopathic manufacturing procedures

  10. Nonlinear modeling of activated sludge process using the Hammerstein-Wiener structure

    Directory of Open Access Journals (Sweden)

    Frącz Paweł


    Full Text Available The paper regards to physical model of the Activated Sludge Process, which is a part of the wastewater treatment. The aim of the study was to describe nitrogen transformation process and the demand of chemical fractions, involved in the ASP process. Moreover, the non-linear relationship between the flow of wastewater and the consumed electrical energy, used by the blowers, was determined. Such analyses are important from the economical and environmental point of view. Assuming that the total power does not change the blower is charging during a year an energy amount of approx. 613 MW. This illustrates in particular the scale of the demand for energy consumption in the biological aeration unit. The aim is to minimize the energy consumption through first building a model of ASP and then through optimization of the overall process by modifying chosen parameter in numerical simulations. In this paper example measurement and analysis results of nitrite and ammonium nitrogen concentrations in the aeration reactor and the active power consumed by blowers for the aeration process were presented. Further the ASP modeling procedure, which uses the Hammerstein-Wiener structure and example verification results were presented. Based on the achieved results it was stated that the developed set of methodologies may be used to improve and expand the overriding control system for system for wastewater treatment plant.

  11. Responsive inverse opal hydrogels for the sensing of macromolecules. (United States)

    Couturier, Jean-Philippe; Sütterlin, Martin; Laschewsky, André; Hettrich, Cornelia; Wischerhoff, Erik


    Dual responsive inverse opal hydrogels were designed as autonomous sensor systems for (bio)macromolecules, exploiting the analyte-induced modulation of the opal's structural color. The systems that are based on oligo(ethylene glycol) macromonomers additionally incorporate comonomers with various recognition units. They combine a coil-to-globule collapse transition of the LCST type with sensitivity of the transition temperature toward molecular recognition processes. This enables the specific detection of macromolecular analytes, such as glycopolymers and proteins, by simple optical methods. While the inverse opal structure assists the effective diffusion even of large analytes into the photonic crystal, the stimulus responsiveness gives rise to strong shifts of the optical Bragg peak of more than 100 nm upon analyte binding at a given temperature. The systems' design provides a versatile platform for the development of easy-to-use, fast, and low-cost sensors for pathogens.

  12. Covalent binding of foreign chemicals to tissue macromolecules. [Acetaminophen

    Energy Technology Data Exchange (ETDEWEB)

    Thorgeirsson, S.S.; Wirth, P.J.


    In vivo and in vitro covalent binding of foreign chemicals to tissue macromolecules via metabolic activation is described, using the analgesic acetaminophen as an example. Acetaminophen is metabolized through a variety of pathways. The arylating metabolite is formed by a cytochrome P-450 dependent N-hydroxylation process. The resulting hydroxamic acid is then conjugated with glutathione, and the resulting conjugate is subsequently excreted as the mercapturic acid in the urine. It is not until the glutathione concentration is reduced to about 20% of the initial concentration that covalent binding of acetaminophen to amino acids of proteins occurs and subsequent liver necrosis is seen. The extent of in vitro binding correlates with treatments that alter hepatic necrosis and in vivo binding, indicating that in vitro binding is a valid index of acetaminophen hepatotoxicity. A simple bacterial test system for detecting chemical carcinogens as mutagens is described.

  13. Parametric Phase-sensitive and Phase-insensitive All-optical Signal Processing on Multiple Nonlinear Platforms - Invited talk

    DEFF Research Database (Denmark)

    Peucheret, Christophe; Da Ros, Francesco; Vukovic, Dragana;

    - compatible fabrication process, degrees of freedom in dispersion engineering, and high nonlinear coecient. However, the detrimental eect of free-carrier absorption induced by two-photon absorp- tion has so far prevented them from being used for the demonstration of phase-sensitive processing. Thanks...

  14. Dataset on coherent control of fields and induced currents in nonlinear multiphoton processes in a nanosphere. (United States)

    McArthur, Duncan; Hourahine, Ben; Papoff, Francesco


    We model a scheme for the coherent control of light waves and currents in metallic nanospheres which applies independently of the nonlinear multiphoton processes at the origin of waves and currents. Using exact mathematical formulae, we calculate numerically with a custom fortran code the effect of an external control field which enable us to change the radiation pattern and suppress radiative losses or to reduce absorption, enabling the particle to behave as a perfect scatterer or as a perfect absorber. Data are provided in tabular, comma delimited value format and illustrate narrow features in the response of the particles that result in high sensitivity to small variations in the local environment, including subwavelength spatial shifts.

  15. Nonlinear Sagnac interferometer based on the four-wave mixing process. (United States)

    Xin, Jun; Liu, Jinming; Jing, Jietai


    A new nonlinear Sagnac interferometer (NSI) is proposed by replacing the beam-splitter in the traditional Sagnac interferometer (TSI) with a four-wave mixing process. Such a NSI has better angular velocity sensitivity than the one of the TSI. The standard quantum limit can be beaten and the Heisenberg Limit can even be reached for the ideal case by the NSI. We study the effect of the losses on the angular velocity sensitivity of the NSI and find that the optimal angular velocity, where the best angular velocity sensitivity can be obtained, of the NSI may be dependent on the losses inside the interferometer. Such a NSI has its advantages compared with the TSI and may find its potential applications in quantum metrology.

  16. Nonlinear Transient Dynamics of Photoexcited Silicon Nanoantenna for Ultrafast All-Optical Signal Processing

    CERN Document Server

    Baranov, Denis G; Milichko, Valentin A; Kudryashov, Sergey I; Krasnok, Alexander E; Belov, Pavel A


    Optically generated electron-hole plasma in high-index dielectric nanostructures was demonstrated as a means of tuning of their optical properties. However, until now an ultrafast operation regime of such plasma driven nanostructures has not been attained. Here, we perform pump-probe experiments with resonant silicon nanoparticles and report on dense optical plasma generation near the magnetic dipole resonance with ultrafast (about 2.5 ps) relaxation rate. Basing on experimental results, we develop an analytical model describing transient response of a nanocrystalline silicon nanoparticle to an intense laser pulse and show theoretically that plasma induced optical nonlinearity leads to ultrafast reconfiguration of the scattering power pattern. We demonstrate 100 fs switching to unidirectional scattering regime upon irradiation of the nanoparticle by an intense femtosecond pulse. Our work lays the foundation for developing ultracompact and ultrafast all-optical signal processing devices.

  17. Nonlinear color-image decomposition for image processing of a digital color camera (United States)

    Saito, Takahiro; Aizawa, Haruya; Yamada, Daisuke; Komatsu, Takashi


    This paper extends the BV (Bounded Variation) - G and/or the BV-L1 variational nonlinear image-decomposition approaches, which are considered to be useful for image processing of a digital color camera, to genuine color-image decomposition approaches. For utilizing inter-channel color cross-correlations, this paper first introduces TV (Total Variation) norms of color differences and TV norms of color sums into the BV-G and/or BV-L1 energy functionals, and then derives denoising-type decomposition-algorithms with an over-complete wavelet transform, through applying the Besov-norm approximation to the variational problems. Our methods decompose a noisy color image without producing undesirable low-frequency colored artifacts in its separated BV-component, and they achieve desirable high-quality color-image decomposition, which is very robust against colored random noise.

  18. Identification of Input Nonlinear Control Autoregressive Systems Using Fractional Signal Processing Approach

    Directory of Open Access Journals (Sweden)

    Naveed Ishtiaq Chaudhary


    Full Text Available A novel algorithm is developed based on fractional signal processing approach for parameter estimation of input nonlinear control autoregressive (INCAR models. The design scheme consists of parameterization of INCAR systems to obtain linear-in-parameter models and to use fractional least mean square algorithm (FLMS for adaptation of unknown parameter vectors. The performance analyses of the proposed scheme are carried out with third-order Volterra least mean square (VLMS and kernel least mean square (KLMS algorithms based on convergence to the true values of INCAR systems. It is found that the proposed FLMS algorithm provides most accurate and convergent results than those of VLMS and KLMS under different scenarios and by taking the low-to-high signal-to-noise ratio.

  19. Identification of Input Nonlinear Control Autoregressive Systems Using Fractional Signal Processing Approach (United States)

    Chaudhary, Naveed Ishtiaq; Raja, Muhammad Asif Zahoor; Khan, Junaid Ali; Aslam, Muhammad Saeed


    A novel algorithm is developed based on fractional signal processing approach for parameter estimation of input nonlinear control autoregressive (INCAR) models. The design scheme consists of parameterization of INCAR systems to obtain linear-in-parameter models and to use fractional least mean square algorithm (FLMS) for adaptation of unknown parameter vectors. The performance analyses of the proposed scheme are carried out with third-order Volterra least mean square (VLMS) and kernel least mean square (KLMS) algorithms based on convergence to the true values of INCAR systems. It is found that the proposed FLMS algorithm provides most accurate and convergent results than those of VLMS and KLMS under different scenarios and by taking the low-to-high signal-to-noise ratio. PMID:23853538

  20. Nonlinear control of an activated sludge aeration process: use of fuzzy techniques for tuning PID controllers. (United States)

    Rodrigo, M A; Seco, A; Ferrer, J; Penya-roja, J M; Valverde, J L


    In this paper, several tuning algorithms, specifically ITAE, IMC and Cohen and Coon, were applied in order to tune an activated sludge aeration PID controller. Performance results of these controllers were compared by simulation with those obtained by using a nonlinear fuzzy PID controller. In order to design this controller, a trial and error procedure was used to determine, as a function of error at current time and at a previous time, sets of parameters (including controller gain, integral time and derivative time) which achieve satisfactory response of a PID controller actuating over the aeration process. Once these sets of data were obtained, neural networks were used to obtain fuzzy membership functions and fuzzy rules of the fuzzy PID controller.

  1. Non-linear multivariable predictive control of an alcoholic fermentation process using functional link networks

    Directory of Open Access Journals (Sweden)

    Luiz Augusto da Cruz Meleiro


    Full Text Available In this work a MIMO non-linear predictive controller was developed for an extractive alcoholic fermentation process. The internal model of the controller was represented by two MISO Functional Link Networks (FLNs, identified using simulated data generated from a deterministic mathematical model whose kinetic parameters were determined experimentally. The FLN structure presents as advantages fast training and guaranteed convergence, since the estimation of the weights is a linear optimization problem. Besides, the elimination of non-significant weights generates parsimonious models, which allows for fast execution in an MPC-based algorithm. The proposed algorithm showed good potential in identification and control of non-linear processes.Neste trabalho um controlador preditivo não linear multivariável foi desenvolvido para um processo de fermentação alcoólica extrativa. O modelo interno do controlador foi representado por duas redes do tipo Functional Link (FLN, identificadas usando dados de simulação gerados a partir de um modelo validado experimentalmente. A estrutura FLN apresenta como vantagem o treinamento rápido e convergência garantida, já que a estimação dos seus pesos é um problema de otimização linear. Além disso, a eliminação de pesos não significativos gera modelos parsimoniosos, o que permite a rápida execução em algoritmos de controle preditivo baseado em modelo. Os resultados mostram que o algoritmo proposto tem grande potencial para identificação e controle de processos não lineares.

  2. In-TFT-Array-Process Micro Defect Inspection Using Nonlinear Principal Component Analysis

    Directory of Open Access Journals (Sweden)

    Zhi-Hao Kang


    Full Text Available Defect inspection plays a critical role in thin film transistor liquid crystal display (TFT-LCD manufacture, and has received much attention in the field of automatic optical inspection (AOI. Previously, most focus was put on the problems of macro-scale Mura-defect detection in cell process, but it has recently been found that the defects which substantially influence the yield rate of LCD panels are actually those in the TFT array process, which is the first process in TFT-LCD manufacturing. Defect inspection in TFT array process is therefore considered a difficult task. This paper presents a novel inspection scheme based on kernel principal component analysis (KPCA algorithm, which is a nonlinear version of the well-known PCA algorithm. The inspection scheme can not only detect the defects from the images captured from the surface of LCD panels, but also recognize the types of the detected defects automatically. Results, based on real images provided by a LCD manufacturer in Taiwan, indicate that the KPCA-based defect inspection scheme is able to achieve a defect detection rate of over 99% and a high defect classification rate of over 96% when the imbalanced support vector machine (ISVM with 2-norm soft margin is employed as the classifier. More importantly, the inspection time is less than 1 s per input image.

  3. Intelligent non-linear modelling of an industrial winding process using recurrent local linear neuro-fuzzy networks

    Institute of Scientific and Technical Information of China (English)



    This study deals with the neuro-fuzzy (NF) modelling of a real industrial winding process in which the acquired NF model can be exploited to improve control performance and achieve a robust fault-tolerant system.A new simulator model is proposed for a winding process using non-linear identification based on a recurrent local linear neuro-fuzzy (RLLNF) network trained by local linear model tree (LOLIMOT),which is an incremental tree-based learning algorithm.The proposed NF models are compared with other known intelligent identifiers,namely multilayer perceptron (MLP) and radial basis function (RBF).Comparison of our proposed non-linear models and associated models obtained through the least square error (LSE) technique (the optimal modelling method for linear systems) confirms that the winding process is a non-linear system.Experimental results show the effectiveness of our proposed NF modelling approach.

  4. A Model Predictive Algorithm for Active Control of Nonlinear Noise Processes

    Directory of Open Access Journals (Sweden)

    Qi-Zhi Zhang


    Full Text Available In this paper, an improved nonlinear Active Noise Control (ANC system is achieved by introducing an appropriate secondary source. For ANC system to be successfully implemented, the nonlinearity of the primary path and time delay of the secondary path must be overcome. A nonlinear Model Predictive Control (MPC strategy is introduced to deal with the time delay in the secondary path and the nonlinearity in the primary path of the ANC system. An overall online modeling technique is utilized for online secondary path and primary path estimation. The secondary path is estimated using an adaptive FIR filter, and the primary path is estimated using a Neural Network (NN. The two models are connected in parallel with the two paths. In this system, the mutual disturbances between the operation of the nonlinear ANC controller and modeling of the secondary can be greatly reduced. The coefficients of the adaptive FIR filter and weight vector of NN are adjusted online. Computer simulations are carried out to compare the proposed nonlinear MPC method with the nonlinear Filter-x Least Mean Square (FXLMS algorithm. The results showed that the convergence speed of the proposed nonlinear MPC algorithm is faster than that of nonlinear FXLMS algorithm. For testing the robust performance of the proposed nonlinear ANC system, the sudden changes in the secondary path and primary path of the ANC system are considered. Results indicated that the proposed nonlinear ANC system can rapidly track the sudden changes in the acoustic paths of the nonlinear ANC system, and ensure the adaptive algorithm stable when the nonlinear ANC system is time variable.

  5. Inferring Instantaneous, Multivariate and Nonlinear Sensitivities for the Analysis of Feedback Processes in a Dynamical System: Lorenz Model Case Study (United States)

    Aires, Filipe; Rossow, William B.; Hansen, James E. (Technical Monitor)


    A new approach is presented for the analysis of feedback processes in a nonlinear dynamical system by observing its variations. The new methodology consists of statistical estimates of the sensitivities between all pairs of variables in the system based on a neural network modeling of the dynamical system. The model can then be used to estimate the instantaneous, multivariate and nonlinear sensitivities, which are shown to be essential for the analysis of the feedbacks processes involved in the dynamical system. The method is described and tested on synthetic data from the low-order Lorenz circulation model where the correct sensitivities can be evaluated analytically.

  6. Schwinger-Dyson equations in large-N quantum field theories and nonlinear random processes

    CERN Document Server

    Buividovich, P V


    We study stochastic methods for solving Schwinger-Dyson equations in large-N quantum field theories. Expectation values of single-trace operators are sampled by stationary probability distributions of so-called nonlinear random processes. The set of all histories of such processes corresponds to the set of all planar diagrams in the perturbative expansion of the theory. We describe stochastic algorithms for summation of planar diagrams in matrix-valued scalar field theory and in the Weingarten model of random planar surfaces on the lattice. For compact field variables, the method does not converge in the physically most interesting weak-coupling limit. In this case one can absorb the divergences into the self-consistent redefinition of expansion parameters. Stochastic solution of the self-consistency conditions can be implemented as a random process with memory. We illustrate this idea on the example of two-dimensional O(N) sigma-model. Extension to non-Abelian lattice gauge theories is discussed.

  7. Extreme-Point Symmetric Mode Decomposition Method for Nonlinear and Non-Stationary Signal Processing

    CERN Document Server

    Wang, Jin-Liang


    To process nonlinear and non-stationary signals, an extreme-point symmetric mode decomposition (ESMD) method is developed. It can be seen as a new alternate of the well-known Hilbert-Huang transform (HHT) method which is widely used nowadays. There are two parts for it. The first part is the decomposition approach which yields a series of intrinsic mode functions (IMFs) together with an optimal adaptive global mean (AGM) curve, the second part is the direct interpolating (DI) approach which yields instantaneous amplitudes and frequencies for the IMFs together with a time-varying energy. Relative to the HHT method it has five characteristics as follows: (1) Different from constructing 2 outer envelopes, its sifting process is implemented by the aid of 1, 2 or 3 inner interpolating curves; (2) It does not decompose the signal to the last trend curve with at most one extreme point, it optimizes the residual component to be an optimal AGM curve which possesses a certain number of extreme points; (3) Its symmetry ...

  8. Fundamental nonlinearities of the reactor-settler interaction in the activated sludge process. (United States)

    Diehl, Stefan; Farås, Sebastian


    The activated sludge process can be modelled by ordinary and partial differential equations for the biological reactors and secondary settlers, respectively. Because of the complexity of such a system, simulation models are most often used to investigate them. However, simulation models cannot give general rules on how to control a complex nonlinear process. For a reduced-order model with only two components, soluble substrate and particulate biomass, general results on steady-state solutions have recently been obtained, such as existence, uniqueness and stability of solutions. The aim of the present paper is to utilize those results to formulate some implications of practical importance. In particular, strategies are described for the manual control of the effluent substrate concentration subject to the constraint that the settler is maintained in normal operation (with a sludge blanket in the thickening zone) in steady state. Such strategies contain how the two control parameters, the recycle and waste volumetric flow ratios, should be chosen for any (steady-state) values of the input variables.

  9. Towards information processing from nonlinear physical chemistry: a synthetic electrochemical cognitive system. (United States)

    Sadeghi, Saman; Thompson, Michael


    It is evident that complex animate materials, which operate far from equilibrium, exhibit sensory responses to the environment through emergent patterns. Formation of such patterns is often the underlying mechanism of an active response to environmental changes and can be interpreted as a result of the distributed parallel information processing taking place within the material. Such emergent patterns are not limited to biological entities; indeed there is a wide range of complex nonlinear dissipative systems which exhibit interesting emergent patterns within a range of parameters. As one example, the present paper describes the detection of emergent phenomena associated with surface electrochemical processes that allow the system to respond to input information through evolving patterns in space and time. Associative mapping of this sort offers the opportunity to devise an electrochemical cognitive system (ECS), where pattern formation can be looked at as a macroscopic phenomenon resulting from the extensive distributive computing that occurs at the microscopic level. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  10. Nonlinear PI Control with Adaptive Interaction Algorithm for Multivariable Wastewater Treatment Process

    Directory of Open Access Journals (Sweden)

    S. I. Samsudin


    Full Text Available The wastewater treatment plant (WWTP is highly known with the nonlinearity of the control parameters, thus it is difficult to be controlled. In this paper, the enhancement of nonlinear PI controller (ENon-PI to compensate the nonlinearity of the activated sludge WWTP is proposed. The ENon-PI controller is designed by cascading a sector-bounded nonlinear gain to linear PI controller. The rate variation of the nonlinear gain kn is automatically updated based on adaptive interaction algorithm. Initiative to simplify the ENon-PI control structure by adapting kn has been proved by significant improvement under various dynamic influents. More than 30% of integral square error and 14% of integral absolute error are reduced compared to benchmark PI for DO control and nitrate in nitrogen removal control. Better average effluent qualities, less number of effluent violations, and lower aeration energy consumption resulted.

  11. Marine-derived biological macromolecule-based biomaterials for wound healing and skin tissue regeneration. (United States)

    Chandika, Pathum; Ko, Seok-Chun; Jung, Won-Kyo


    Wound healing is a complex biological process that depends on the wound condition, the patient's health, and the physicochemical support given through external materials. The development of bioactive molecules and engineered tissue substitutes to provide physiochemical support to enhance the wound healing process plays a key role in advancing wound-care management. Thus, identification of ideal molecules in wound treatment is still in progress. The discovery of natural products that contain ideal molecules for skin tissue regeneration has been greatly advanced by exploration of the marine bioenvironment. Consequently, tremendously diverse marine organisms have become a great source of numerous biological macromolecules that can be used to develop tissue-engineered substitutes with wound healing properties. This review summarizes the wound healing process, the properties of macromolecules from marine organisms, and the involvement of these molecules in skin tissue regeneration applications.

  12. ELF Nonlinear Noise Processing Experimental Measurements, Part 2 - Synoptic Sample of Diurnal and Seasonal Noise Variation in Norway (United States)


    clipping level. The nonlinear processing method described in this report provides at least 10 dB of S/N improvement over the performance obtained without...0001s Ii 0110 151 to-, ,n o 151. 1 iK) Il O( M I ( lf clipper performance was also eviden -t in the January 1974 data. Table 7 contains statisti- cal

  13. Growth of thin films of organic nonlinear optical materials by vapor growth processes - An overview and examination of shortfalls (United States)

    Frazier, D. O.; Penn, B. G.; Witherow, W. K.; Paley, M. S.


    Research on the growth of second- and third-order nonlinear optical (NLO) organic thin film by vapor deposition is reviewed. Particular attention is given to the experimental methods for growing thin films of p-chlorophenylurea, diacetylenes, and phthalocyanines; characteristics of the resulting films; and approaches for advancing thin film technology. It is concluded that the growth of NLO thin films by vapor processes is a promising method for the fabrication of planar waveguides for nonlinear optical devices. Two innovative approaches are proposed including a method of controlling the input beam frequency to maximize nonlinear effects in thin films and single crystals, and the alternate approach to the molecular design of organic NLO materials by increasing the transition dipole moment between ground and excited states of the molecule.

  14. Optimal Constant-Stress Accelerated Degradation Test Plans Using Nonlinear Generalized Wiener Process

    Directory of Open Access Journals (Sweden)

    Zhen Chen


    Full Text Available Accelerated degradation test (ADT has been widely used to assess highly reliable products’ lifetime. To conduct an ADT, an appropriate degradation model and test plan should be determined in advance. Although many historical studies have proposed quite a few models, there is still room for improvement. Hence we propose a Nonlinear Generalized Wiener Process (NGWP model with consideration of the effects of stress level, product-to-product variability, and measurement errors for a higher estimation accuracy and a wider range of use. Then under the constraints of sample size, test duration, and test cost, the plans of constant-stress ADT (CSADT with multiple stress levels based on the NGWP are designed by minimizing the asymptotic variance of the reliability estimation of the products under normal operation conditions. An optimization algorithm is developed to determine the optimal stress levels, the number of units allocated to each level, inspection frequency, and measurement times simultaneously. In addition, a comparison based on degradation data of LEDs is made to show better goodness-of-fit of the NGWP than that of other models. Finally, optimal two-level and three-level CSADT plans under various constraints and a detailed sensitivity analysis are demonstrated through examples in this paper.

  15. New Space Weather and Nonlinear Waves and Processes Prize announced for 2013 (United States)

    Thompson, Victoria


    At the 2011 Fall Meeting in San Francisco, Calif., AGU announced the creation of a new award: the Space Weather and Nonlinear Waves and Processes Prize. The prize, which is being made possible by a generous contribution from longtime AGU members and NASA Jet Propulsion Laboratory (JPL), California Institute of Technology, scientists Bruce Tsurutani and Olga Verkhoglyadova, will recognize an AGU member scientist and will come with a $10,000 award. Tsurutani has served as a researcher with JPL since 1972 and is currently a senior research scientist. He was also the president of AGU's Space Physics and Aeronomy section from 1990 to 1992 and is a recipient of AGU's John Adam Fleming Medal, given “for original research and technical leadership in geomagnetism, atmospheric electricity, aeronomy, space physics, and related sciences.” Verkhoglyadova served as a professor of space physics in the Department of Astrophysics and Space Physics at Taras Shevchenko National University of Kyiv, in the Ukraine, prior to coming to the United States. Their leadership and dedication to AGU and to their field are apparent in their passion for this prize.

  16. Three novel high-resolution nonlinear methods for fast signal processing (United States)

    Belkić, Dž.; Dando, P. A.; Main, J.; Taylor, H. S.


    Three novel nonlinear parameter estimators are devised and implemented for accurate and fast processing of experimentally measured or theoretically generated time signals of arbitrary length. The new techniques can also be used as powerful tools for diagonalization of large matrices that are customarily encountered in quantum chemistry and elsewhere. The key to the success and the common denominator of the proposed methods is a considerably reduced dimensionality of the original data matrix. This is achieved in a preprocessing stage called beamspace windowing or band-limited decimation. The methods are decimated signal diagonalization (DSD), decimated linear predictor (DLP), and decimated Padé approximant (DPA). Their mutual equivalence is shown for the signals that are modeled by a linear combination of time-dependent damped exponentials with stationary amplitudes. The ability to obtain all the peak parameters first and construct the required spectra afterwards enables the present methods to phase correct the absorption mode. Additionally, a new noise reduction technique, based upon the stabilization method from resonance scattering theory, is proposed. The results obtained using both synthesized and experimental time signals show that DSD/DLP/DPA exhibit an enhanced resolution power relative to the standard fast Fourier transform. Of the three methods, DPA is found to be the most efficient computationally.

  17. Energy conversion in isothermal nonlinear irreversible processes - struggling for higher efficiency (United States)

    Ebeling, W.; Feistel, R.


    First we discuss some early work of Ulrike Feudel on structure formation in nonlinear reactions including ions and the efficiency of the conversion of chemical into electrical energy. Then we give some survey about isothermal energy conversion from chemical to higher forms of energy like mechanical, electrical and ecological energy. Isothermal means here that there are no temperature gradients within the model systems. We consider examples of energy conversion in several natural processes and in some devices like fuel cells. Further, as an example, we study analytically the dynamics and efficiency of a simple "active circuit" converting chemical into electrical energy and driving currents which is roughly modeling fuel cells. Finally we investigate an analogous ecological system of Lotka-Volterra type consisting of an "active species" consuming some passive "chemical food". We show analytically for both these models that the efficiency increases with the load, reaches values higher then 50 percent in a narrow regime of optimal load and goes beyond some maximal load abruptly to zero.

  18. Does the cerebral cortex exploit high dimensional, non-linear dynamics for information processing?

    Directory of Open Access Journals (Sweden)

    Wolf Singer


    Full Text Available The discovery of stimulus induced synchronisation in the visual cortex suggested the possibility that the relations among low-level stimulus features are encoded by the temporal relationship between neuronal discharges. In this framework, temporal coherence is considered a signature of perceptual grouping. This insight triggered a large number of experimental studies which sought to investigate the relationship between temporal coordination and cognitive functions. While some core predictions derived from the initial hypothesis were confirmed, these studies, also revealed a rich dynamical landscape beyond simple coherence whose role in signal processing is still poorly understood. In this paper a framework is presented which establishes links between the various manifestations of cortical dynamics by assigning specific coding functions to low dimensional dynamic features such as synchronized oscillations and phase shifts on the one hand and high dimensional non-linear, non-stationary dynamics on the other. The data serving as basis for this synthetic approach have been obtained with chronic multisite recordings from the visual cortex of anesthetized cats and from monkeys trained to solve cognitive tasks. It is proposed that the low dimensional dynamics characterized by synchronized oscillations and large-scale correlations are sub-states that represent the results of computations performed in the high dimensional state space provided by recurrently coupled networks.

  19. The Circle of Dust: From Nanoparticles to Macromolecules and Beyond (United States)

    Micelotta, E.; Jones, A.; Bocchio, M.; Cami, J.; Peeters, E.; Bernard-Salas, J.

    There is increasing observational evidence that a non-negligible fraction of the cosmic carbon is locked up into macromolecules and nanoparticles. Carbonaceous nanoparticles and Hydrogenated Amorphous Carbon (HAC) nanoparticles represent one of the main components of interstellar dust. HAC nanoparticles have been proposed as a viable carrier for the Unidentified InfraRed (UIR) bands, which dominate the mid-infrared spectrum of almost any astronomical object. Fullerene molecules C60 and C70 have been detected in various circumstellar and interstellar environments. We present some of our recent results about the evolution of such carbonaceous structures and the possible connections between each other. We show how photo-processing of HAC nanoparticles can lead to the formation of C60 and C70 in space. There the low density of the gas precludes the formation of fullerene materials following known vaporization or combustion synthesis routes, even on astronomical timescales. We then discuss the processing of small hydrocarbon dust by energetic ions and electrons under extreme conditions, e.g., in shocked regions. Finally, we derive the astrophysical implications of such processing in terms of the observed emission.

  20. Sandwich complex-containing macromolecules: property tunability through versatile synthesis. (United States)

    Abd-El-Aziz, Alaa S; Agatemor, Christian; Etkin, Nola


    Sandwich complexes feature unique properties as the physical and electronic properties of a hydrocarbon ligand or its derivative are integrated into the physical, electronic, magnetic, and optical properties of a metal. Incorporation of these complexes into macromolecules results in intriguing physical, electrical, and optical properties that were hitherto unknown in organic-based macromolecules. These properties are tunable through well-designed synthetic strategies. This review surveys many of the synthetic approaches that have resulted in tuning the properties of sandwich complex-containing macromolecules. While the past two decades have seen an ever-growing number of research publications in this field, gaps remain to be filled. Thus, we expect this review to stimulate research interest towards bridging these gaps, which include the insolubility of some of these macromolecules as well as expanding the scope of the sandwich complexes. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Ligand Binding to Macromolecules: Allosteric and Sequential Models of Cooperativity. (United States)

    Hess, V. L.; Szabo, Attila


    A simple model is described for the binding of ligands to macromolecules. The model is applied to the cooperative binding by hemoglobin and aspartate transcarbamylase. The sequential and allosteric models of cooperative binding are considered. (BB)

  2. Organized monolayers of biological macromolecules on Au(111) surfaces

    DEFF Research Database (Denmark)

    Zhang, Jingdong; Chi, Qijin; Nielsen, Jens Ulrik


    Single-crystal electrochemistry and scanning tunneling microscopy directly in aqueous electrolyte solution (in situ STM) are established in physical electrochemistry but new in studies of adsorption and interfacial electrochemistry of biological macromolecules. These high-resolution techniques have...

  3. Effect of anionic macromolecules on intestinal permeability of furosemide. (United States)

    Valizadeh, Hadi; Fahimfar, Hadi; Ghanbarzadeh, Saeed; Islambulchilar, Ziba; Zakeri-Milani, Parvin


    Furosemide is an anionic molecule and has very low absorption in gastro intestinal tract. The aim of this study was to investigate the effect of anionic macromolecules on the intestinal permeability of Furosemide. The intestinal permeability of Furosemide was determined using single-pass intestinal perfusion technique in rats. Briefly a jejunal segment of ∼10 cm was isolated and cannulated in both ends for inlet and outlet solution. The perfusate was collected every 10 min and samples were analyzed using the RP-HPLC method. Test samples containing furosemide and two anionic macromolecules, sodium carboxy methyl cellulose and sodium alginate, at different concentrations were used. The obtained data showed that existence of Sodium carboxy methyl cellulose significantly increased the Peff values in all three investigated concentrations (p macromolecules at specific concentrations could alter the permeability of anionic drugs across the biological membranes. Donnan phenomenon and chelating property of macromolecules could be attributed to the observed effect.

  4. Nonlinear Dynamics of Photonics for Optical Signal Processing - Optical Frequency Conversion and Optical DSB-to-SSB Conversion (United States)


    processing - optical frequency conversion and optical DSB -to-SSB conversion 5a. CONTRACT NUMBER FA2386-14-1-0006 5b. GRANT NUMBER Grant 134113...nonlinear dynamics of semiconductor lasers for certain optical signal processing functionalities, including optical DSB -to-SSB conversion, photonic...conversion and optical DSB -to-SSB conversion Performance Period May 30, 2014 ~ May 29, 2015 Principal Investigator Name: Sheng-Kwang Hwang Position

  5. [Non-equilibrium thermodynamic separation theory of nonlinear chromatography. II. The 0-1 model for nonlinear-mass transfer kinetic processes]. (United States)

    Liang, Heng; Jia, Zhenbin


    In the optimal design and control of preparative chromatographic processes, the obstacles appear when one tries to link the Wilson' s framework of chromatographic theories based on partial differential equations (PDEs) with the Eulerian presentation to optimal control approaches based on discrete time states, such as Markov decision processes (MDP) or Model predictive control (MPC). In this paper, the 0-1 model is presented to overcome the obstacles for nonlinear transport chromatography (NTC). With the Lagrangian-Eulerian description (L-ED), one solute cell unit is split into two solute cells, one (SCm) in the mobile phase with the linear velocity of the mobile phase, and the other (SCs) in the stationary phase with zero-velocity. The thermodynamic state vector, S(k), which comprises four vector components, i.e., the sequence number, the position and the local solute concentrations in both SCms and SCses, is introduced to describe the local thermodynamic path (LTP) and the macroscopical thermodynamic path (MTP). For the NTC, the LTP is designed for a solute zone to evolve from the state, S(k), to the virtual migration state, S(M), undergoing the virtual net migration sub-process, and then to the state, S(k+1), undergoing the virtual net inter phase mass transfer sub-process in a short time interval. Complete thermodynamic state iterations with the Markov characteristics are derived by using the local equilibrium isotherm and the local lumped mass transfer coefficient. When the local thermodynamic equilibrium is retained, excellent properties, such as consistency, stability, conservation, accuracy, etc., of the numerical solution of the 0-1 model are observed in the theoretical analysis and in the numerical experiments of the nonlinear ideal chromatography. It is found that the 0-1 model could properly link up with the MDP or optimal control approaches based on discrete time states.

  6. Electrically assisted delivery of macromolecules into the corneal epithelium


    HAO, JINSONG; Li, S. Kevin; Liu, Chia-Yang; Kao, Winston W. Y.


    Electrically assisted delivery is noninvasive and has been investigated in a number of ocular drug delivery studies. The objectives of this study were to examine the feasibility of electrically assisted delivery of macromolecules such as small interfering RNA (siRNA) into the corneal epithelium, to optimize the iontophoresis and electroporation methods, and to study the mechanisms of corneal iontophoresis for macromolecules. Anodal and cathodal iontophoresis, electroporation and their combina...

  7. The Expansion of Dynamic Solving Process About a Class of Non-linear Programming Problems

    Institute of Scientific and Technical Information of China (English)

    ZANG Zhen-chun


    In this paper, we research non-linear programming problems which have a given specialstructure, some simple forms of this kind structure have been solved in some papers, here we focus on othercomplex ones.

  8. Identification of damage in a suspension component using narrowband and broadband nonlinear signal processing techniques (United States)

    Haroon, Muhammad; Adams, Douglas E.


    Fatigue tests on a stabilizer bar link of an automotive suspension system are used to initiate a crack and grow the crack size. During these tests, slow sine sweeps are used to extract narrowband restoring forces across the stabilizer bar link. The restoring forces are shown to characterize the nonlinear changes in component internal forces due to crack growth. Broadband frequency response domain techniques are used to analyze the durability response data. Nonlinear frequency domain models of the dynamic transmissibility across the cracked region are shown to change as a function of crack growth. Higher order spectra are used to show the increase in nonlinear coupling of response frequency components with the appearance and growth of the crack. It is shown that crack growth can be detected and characterized by the changes in nonlinear indicators.

  9. Submillimeter wave spectroscopy of biological macromolecules (United States)

    Globus, Tatiana


    The recently emergence of submillimeter-wave or terahertz (THz) spectroscopy of biological molecules has demonstrated the capability to detect low-frequency internal molecular vibrations involving the weakest hydrogen bonds of the DNA base pairs and/or non-bonded interactions. These multiple bonds, although having only ˜ 5% of the strength of covalent bonds, stabilize the structure of bio-polymers, by holding the two strands of the DNA double helix together, or polypeptides together in different secondary structure conformations. There will be a review of THz-frequency transmission (absorption) results for biological materials obtained from Fourier Transform Infrared (FTIR) spectroscopy during the last few years^1,2. Multiple resonances, due to low frequency vibrational modes within biological macromolecules, have been unambiguously demonstrated in qualitative agreement with theoretical prediction, thereby confirming the fundamental physical nature of observed resonance features. The discovery of resonance character of interaction between THz radiation and biological materials opens many possible applications for THz spectroscopy technique in biological sensing and biomedicine using multiple resonances as distinctive spectral fingerprints. However, many issues still require investigation. Kinetics of interactions with radiation at THz has not been studied and vibrational lifetimes have not been measured directly as a function of frequency. The strength of resonant modes of bio-molecules in aqueous environment and strong dependence of spectra on molecular orientation need explanation. Vibrational modes have not been assigned to specific motions within molecules. THz spectroscopy of bio-polymers makes it only in first steps. 1. T. Globus, D. Woolard, M. Bykhovskaia, B. Gelmont, L. Werbos, A. Samuels. International Journal of High Speed Electronics and Systems (IJHSES), 13, No. 4, 903-936 (2003). 2. T. Globus, T. Khromova, D. Woolard and B. Gelmont. Proceedings of

  10. Electrically assisted delivery of macromolecules into the corneal epithelium. (United States)

    Hao, Jinsong; Li, S Kevin; Liu, Chia-Yang; Kao, Winston W Y


    Electrically assisted delivery is noninvasive and has been investigated in a number of ocular drug delivery studies. The objectives of this study were to examine the feasibility of electrically assisted delivery of macromolecules such as small interfering RNA (siRNA) into the corneal epithelium, to optimize the iontophoresis and electroporation methods, and to study the mechanisms of corneal iontophoresis for macromolecules. Anodal and cathodal iontophoresis, electroporation and their combinations were the methods examined with mice in vivo. Cyanine 3 (Cy3)-labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) siRNA and fluorescein isothiocyanate (FITC)-labeled dextran of different molecular weights (4-70 kDa) were the macromolecules studied. Microscopy and histology after cryostat sectioning were used to analyze and compare the delivery of the macromolecules to the cornea. Iontophoresis was effective in delivering siRNA and dextran up to 70 kDa into the cornea. The electroporation method studied was less effective than that of iontophoresis. Although both iontophoresis and electroporation alone can deliver the macromolecules into the cornea, these methods alone were not as effective as the combination of iontophoresis and electroporation (iontophoresis followed by electroporation). The significant enhancement of dextran delivery in anodal iontophoresis suggests that electroosmosis can be a significant flux-enhancing mechanism during corneal iontophoresis. These results illustrate the feasibility of electrically assisted delivery of macromolecules such as siRNA into the cornea.

  11. Uncovering Molecular Relaxation Processes with Nonlinear Spectroscopies in the Deep UV (United States)

    West, Brantley Andrew

    Conical intersections mediate internal conversion dynamics that compete with even the fastest nuclear motions in molecular systems. Traditional kinetic models do not apply in this regime of commensurate electronic and nuclear motion because the surroundings do not maintain equilibrium throughout the relaxation process. This dissertation focuses on uncovering the physics associated with vibronic interactions at conical intersections. Of particular interest are coherent nuclear motions driven by steep excited state potential energy gradients. Technical advances have only recently made these dynamics accessible in many systems including DNA nucleobases and cyclic polyene molecules. Optical analogues of multidimensional NMR spectroscopies have recently yielded transformative insight in relaxation processes ranging from energy transfer in photosynthesis to bond making and breaking in liquids. Prior to the start of this research, such experiments had only been conducted at infrared and visible wavelengths. Applications in the ultraviolet were motivated by studies of numerous biological systems (e.g., DNA, proteins), but had been challenged by technical issues. The work presented in this dissertation combines pulse generation techniques developed in the optical physics community with spectroscopic techniques largely pioneered by physical chemists to implement two-dimensional ultraviolet spectroscopy (2DUV). This technique is applied at the shortest wavelengths and with the best signal-to-noise ratios reported to date. Sub-picosecond excited state deactivation processes provide photo stability to the DNA double helix. Vibrational energy transfer from the solute to surrounding solvent enables relaxation of the highly non-equilibrium ground state produced by fast internal conversion. In this dissertation, nonlinear spectroscopies carried out at cryogenic temperatures are used to uncover the particular nuclear modes in the solvent that primarily accept vibrational energy from

  12. Nonlinear solution for radiation boundary condition of heat transfer process in human eye. (United States)

    Dehghani, A; Moradi, A; Dehghani, M; Ahani, A


    In this paper we propose a new method based on finite element method for solving radiation boundary condition of heat equation inside the human eye and other applications. Using this method, we can solve heat equation inside human eye without need to model radiation boundary condition to a robin boundary condition. Using finite element method we can obtain a nonlinear equation, and finally we use nonlinear algorithm to solve it. The human eye is modeled as a composition of several homogeneous regions. The Ritz method in the finite element method is used for solving heat differential equation. Applying the boundary conditions, the heat radiation condition and the robin condition on the cornea surface of the eye and on the outer part of sclera are used, respectively. Simulation results of solving nonlinear boundary condition show the accuracy of the proposed method.

  13. Nonlinear reflection process of linearly-polarized, broadband Alfv\\'en waves in the fast solar wind

    CERN Document Server

    Shoda, Munehito


    Using one-dimensional numerical simulations, we study the elementary process of Alfv\\'{e}n wave reflection in a uniform medium, including nonlinear effects. In the linear regime, Alfv\\'{e}n wave reflection is triggered only by the inhomogeneity of the medium, whereas in the nonlinear regime, it can occur via nonlinear wave-wave interactions. Such nonlinear reflection (backscattering) is typified by decay instability. In most studies of decay instabilities, the initial condition has been a circularly polarized Alfv\\'{e}n wave. In this study we consider a linearly polarized Alfv\\'en wave, which drives density fluctuations by its magnetic pressure force. For generality, we also assume a broadband wave with a red-noise spectrum. In the data analysis, we decompose the fluctuations into characteristic variables using local eigenvectors, thus revealing the behaviors of the individual modes. Different from circular-polarization case, we find that the wave steepening produces a new energy channel from the parent Alfv\\...

  14. Observation of nonlinear wave decay processes in the solar wind by the AMPTE IRM plasma wave experiment (United States)

    Koons, H. C.; Roeder, J. L.; Bauer, O. H.; Haerendel, G.; Treumann, R.


    Nonlinear wave decay processes have been detected in the solar wind by the plasma wave experiment aboard the Active Magnetospheric Particle Tracer Explorers (AMPTE) IRM spacecraft. The main process is the generation of ultralow-frequency ion acoustic waves from the decay of Langmuir waves near the electron plasma frequency. Frequently, this is accompanied by an enhancement of emissions near twice the plasma frequency. This enhancement is most likely due to the generation of electromagnetic waves from the coalescence of two Langmuir waves. These processes occur within the electron foreshock in front of the earth's bow shock.

  15. Efficient conversion from infrared to red light by cascaded nonlinear optical processes using an aperiodically poled lithium niobate crystal

    Directory of Open Access Journals (Sweden)

    Juan Eduardo González


    Full Text Available We present a scheme for conversion of pulsed light from the infrared to the red spectral region, using an aperiodically poled ferroelectric crystal within a resonant cavity in which two cascaded nonlinear optical processes occur when pumped with a pulsed Nd:YAG laser. This device emits 9 ns pulses of over 1 mJ at 710 nm and is a viable source for future biomedical applications.

  16. Verification of nonlinear dynamic structural test results by combined image processing and acoustic analysis (United States)

    Tene, Yair; Tene, Noam; Tene, G.


    An interactive data fusion methodology of video, audio, and nonlinear structural dynamic analysis for potential application in forensic engineering is presented. The methodology was developed and successfully demonstrated in the analysis of heavy transportable bridge collapse during preparation for testing. Multiple bridge elements failures were identified after the collapse, including fracture, cracks and rupture of high performance structural materials. Videotape recording by hand held camcorder was the only source of information about the collapse sequence. The interactive data fusion methodology resulted in extracting relevant information form the videotape and from dynamic nonlinear structural analysis, leading to full account of the sequence of events during the bridge collapse.

  17. A 3-D nonlinear recursive digital filter for video image processing (United States)

    Bauer, P. H.; Qian, W.


    This paper introduces a recursive 3-D nonlinear digital filter, which is capable of performing noise suppression without degrading important image information such as edges in space or time. It also has the property of unnoticeable bandwidth reduction immediately after a scene change, which makes the filter an attractive preprocessor to many interframe compression algorithms. The filter consists of a nonlinear 2-D spatial subfilter and a 1-D temporal filter. In order to achieve the required computational speed and increase the flexibility of the filter, all of the linear shift-variant filter modules are of the IIR type.

  18. Study of nonlinear processes of a large experimental thermoacoustic-Stirling heat engine by using computational fluid dynamics (United States)

    Yu, G. Y.; Luo, E. C.; Dai, W.; Hu, J. Y.


    This article focuses on using computational fluid dynamics (CFD) method to study several important nonlinear phenomenon and processes of a large experimental thermoacoustic-Stirling heat engine. First, the simulated physical model was introduced, and the suitable numerical scheme and algorithm for the time-dependent compressible thermoacoustic system was determined through extensive numerical tests. Then, the simulation results of the entire evolution process of self-excited thermoacoustic oscillation and the acoustical characteristics of pressure and velocity waves were presented and analyzed. Especially, the onset temperature and the saturation process of dynamic pressure were captured by the CFD simulation. In addition, another important nonlinear phenomenon accompanying the acoustic wave, which is the steady mass flow through the traveling-wave loop inside the thermoacoustic engine, was studied. To suppress the steady mass flow numerically, a fan model was adopted in the simulation. Finally, the multidimensional effects of vortex formation in the thermal buffer tube and other components were displayed numerically. Most importantly, a substantial comparison between the simulation and experiments was made, which demonstrated well the validity and powerfulness of the CFD simulation for characterizing several complicated nonlinear phenomenon involved in the self-excited thermoacoustic heat engine.

  19. On the stability and dynamics of stochastic spiking neuron models: Nonlinear Hawkes process and point process GLMs (United States)

    Truccolo, Wilson


    Point process generalized linear models (PP-GLMs) provide an important statistical framework for modeling spiking activity in single-neurons and neuronal networks. Stochastic stability is essential when sampling from these models, as done in computational neuroscience to analyze statistical properties of neuronal dynamics and in neuro-engineering to implement closed-loop applications. Here we show, however, that despite passing common goodness-of-fit tests, PP-GLMs estimated from data are often unstable, leading to divergent firing rates. The inclusion of absolute refractory periods is not a satisfactory solution since the activity then typically settles into unphysiological rates. To address these issues, we derive a framework for determining the existence and stability of fixed points of the expected conditional intensity function (CIF) for general PP-GLMs. Specifically, in nonlinear Hawkes PP-GLMs, the CIF is expressed as a function of the previous spike history and exogenous inputs. We use a mean-field quasi-renewal (QR) approximation that decomposes spike history effects into the contribution of the last spike and an average of the CIF over all spike histories prior to the last spike. Fixed points for stationary rates are derived as self-consistent solutions of integral equations. Bifurcation analysis and the number of fixed points predict that the original models can show stable, divergent, and metastable (fragile) dynamics. For fragile models, fluctuations of the single-neuron dynamics predict expected divergence times after which rates approach unphysiologically high values. This metric can be used to estimate the probability of rates to remain physiological for given time periods, e.g., for simulation purposes. We demonstrate the use of the stability framework using simulated single-neuron examples and neurophysiological recordings. Finally, we show how to adapt PP-GLM estimation procedures to guarantee model stability. Overall, our results provide a

  20. Mathematical modeling suggests that periodontitis behaves as a non-linear chaotic dynamical process

    NARCIS (Netherlands)

    Papantonopoulos, G.H.; Takahashi, K.; Bountis, T.; Loos, B.G.


    Background: This study aims to expand on a previously presented cellular automata model and further explore the non-linear dynamics of periodontitis. Additionally the authors investigated whether their mathematical model could predict the two known types of periodontitis, aggressive (AgP) and

  1. The Nonlinear Interaction Process in the Wave Assimilation Model and Its Experiments

    Institute of Scientific and Technical Information of China (English)

    杨永增; 纪永刚; 袁业立


    This paper presents a composite interaction formula based on the discrete-interactionoperator of wave-wave nonlinear interaction for deriving its adjoint source function in the wave assimilation model. Assimilation experiments were performed using the significant wave heights observed by the TOPES/POSEIDON satellite, and the gradient distribution in the physical space wasalso analyzed preliminarily.

  2. Mathematical modeling suggests that periodontitis behaves as a non-linear chaotic dynamical process

    NARCIS (Netherlands)

    Papantonopoulos, G.H.; Takahashi, K.; Bountis, T.; Loos, B.G.


    Background: This study aims to expand on a previously presented cellular automata model and further explore the non-linear dynamics of periodontitis. Additionally the authors investigated whether their mathematical model could predict the two known types of periodontitis, aggressive (AgP) and chroni

  3. Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules

    Directory of Open Access Journals (Sweden)

    Jing Kang


    Full Text Available H-aggregates of the cyanine dye Cy5 are formed during covalent linkage to the cationic macromolecule Poly(allylamine (PAH. The nonfluorescent H-aggregates strongly restrict the usage of the dye for analytical purposes and prevent a quantitative determination of the labeled macromolecules. The behavior of the H-aggregates has been studied by investigation of the absorption and fluorescence spectra of the dye polymer in dependence on solvent, label degree and additional sulfonate groups. H-aggregate formation is caused by an inhomogeneous distribution of the Cy5 molecules on the polymer chain. The H-aggregates can be destroyed by conformational changes of the PAH induced by interactions with polyanions or in organic solvents. It has been found that the polymer labeling process in high content of organic solvents can prevent the formation of H-aggregates. The results offer a better understanding and improvement of the use of the Cy5 dye for labeling purposes in fluorescence detection of macromolecules.


    Directory of Open Access Journals (Sweden)

    P. Y. Rogov


    Full Text Available The paper deals with mathematical model of linear and nonlinear processes occurring at the propagation of femtosecond laser pulses in the vitreous of the human eye. Methods of computing modeling are applied for the nonlinear spectral equation solution describing the dynamics of a two-dimensional TE-polarized radiation in a homogeneous isotropic medium with cubic fast-response nonlinearity without the usage of slowly varying envelope approximation. Environments close to the optical media parameters of the eye were used for the simulation. The model of femtosecond radiation propagation takes into account the process dynamics for dispersion broadening of pulses in time and the occurence of the self-focusing near the retina when passing through the vitreous body of the eye. Dependence between the pulse duration on the retina has been revealed and the duration of the input pulse and the values of power density at which there is self-focusing have been found. It is shown that the main mechanism of radiation damage with the use of titanium-sapphire laser is photoionization. The results coincide with those obtained by the other scientists, and are usable for creation Russian laser safety standards for femtosecond laser systems.

  5. Physicochemical Properties of Ion Pairs of Biological Macromolecules. (United States)

    Iwahara, Junji; Esadze, Alexandre; Zandarashvili, Levani


    Ion pairs (also known as salt bridges) of electrostatically interacting cationic and anionic moieties are important for proteins and nucleic acids to perform their function. Although numerous three-dimensional structures show ion pairs at functionally important sites of biological macromolecules and their complexes, the physicochemical properties of the ion pairs are not well understood. Crystal structures typically show a single state for each ion pair. However, recent studies have revealed the dynamic nature of the ion pairs of the biological macromolecules. Biomolecular ion pairs undergo dynamic transitions between distinct states in which the charged moieties are either in direct contact or separated by water. This dynamic behavior is reasonable in light of the fundamental concepts that were established for small ions over the last century. In this review, we introduce the physicochemical concepts relevant to the ion pairs and provide an overview of the recent advancement in biophysical research on the ion pairs of biological macromolecules.

  6. Tailoring liquid crystalline lipid nanomaterials for controlled release of macromolecules. (United States)

    Bisset, Nicole B; Boyd, Ben J; Dong, Yao-Da


    Lipid-based liquid crystalline materials are being developed as drug delivery systems. However, the use of these materials for delivery of large macromolecules is currently hindered by the small size of the water channels in these structures limiting control over diffusion behaviour. The addition of the hydration-modulating agent, sucrose stearate, to phytantriol cubic phase under excess water conditions incrementally increased the size of these water channels. Inclusion of oleic acid enabled further control of swelling and de-swelling of the matrix via a pH triggerable system where at low pH the hexagonal phase is present and at higher pH the cubic phase is present. Fine control over the release of various sized model macromolecules is demonstrated, indicating future application to controlled loading and release of large macromolecules such as antibodies. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Considering Process Nonlinearity in Dual-Point Composition Control of a High-Purity Ideal Heat Integrated Distillation Column

    Institute of Scientific and Technical Information of China (English)


    Dual-point composition control for a high-purity ideal heat integrated distillation column (HIDiC) is addressed in this work. Three measures are suggested and combined for overcoming process inherent nonlinearities:(1) variable scaling; (2) multi-model representation of process dynamics and (3) feedforward compensation. These strategies can offer the developed control systems with several distinct advantages: (1) capability of dealing with severe disturbances; (2) tight tuning of controller parameters and (3) high robustness with respect to variation of operating conditions. Simulation results demonstrate the effectiveness of the proposed methodology.

  8. Intellective high-precision macromolecule resistance temperature/humidity instrument (United States)

    Liu, Guixiong; Zhou, Qinhe; Kuang, Yongcong; Xu, Jing; Zeng, Zhixin


    Considering that the resistance of macromolecule resistor varies in a wide range and humidity sensor component is sensitive to temperature as well, a intelligent high- precision macromolecule resistance temperature/humidity instrument was proposed in this paper, the instrument is based on the integration of frequency-and-period-measuring method, and sensing characteristic calculation and compensation using interpolation. Practical applications show that the instrument has the advantages of high precision, simple peripheral circuit, low cost, suitability for remote measurement, strong ability of anti-interference and wide operation range.


    Institute of Scientific and Technical Information of China (English)

    WEI Gaoyuan


    The shape asymmetry of gaussian models of linear and circular macromolecules has been numerically invesigated in terms of asphericity and prolateness parameters. These parameters are found to decrease with increasing length for the macromolecule either confined to a plane or in three dimensions. The effect of dimensionality on these parameters is visible only for low dimensions and is generally weak. As dimensionality goes to infinity, it is found that asphericity and prolateness for both chains and rings approach slowly yet descendingly values of corresponding asphericity and prolateness factors, with the exception of the chain which shows a minimum value of asphericity when the embedding space has a dimensionality of four.

  10. Discretisation of the non-linear heat transfer equation for food freezing processes using orthogonal collocation on finite elements

    Directory of Open Access Journals (Sweden)

    E. D. Resende


    Full Text Available The freezing process is considered as a propagation problem and mathematically classified as an "initial value problem." The mathematical formulation involves a complex situation of heat transfer with simultaneous changes of phase and abrupt variation in thermal properties. The objective of the present work is to solve the non-linear heat transfer equation for food freezing processes using orthogonal collocation on finite elements. This technique has not yet been applied to freezing processes and represents an alternative numerical approach in this area. The results obtained confirmed the good capability of the numerical method, which allows the simulation of the freezing process in approximately one minute of computer time, qualifying its application in a mathematical optimising procedure. The influence of the latent heat released during the crystallisation phenomena was identified by the significant increase in heat load in the early stages of the freezing process.

  11. Nonlinear analysis on the coupling process of electromagnetic vibrator and earth

    Institute of Scientific and Technical Information of China (English)

    CHEN; Zubin; TENG; Jiwen; LIN; Jun; ZHANG; Linhang; JIANG


    The linear model based on the hydraulic pressure vibrator has been no longer adaptable to the electromagnetic vibrator. In order to realize the effective transmission of the limited energy from the vibrator to the ground, it is important to study the coupling model of the electromagnetic vibrator and the earth. In this paper, a nonlinear restore term was introduced to the coupling model because of the existence of a large amount of harmonics in the vibrator baseplate. The nonlinear vibration analysis was applied to the model by the multiscale method. In the course of energy transmission from the vibrator to the ground, ultraharmonic resonance was used to explain the generation of harmonics. An improved scheme was advanced to select the cross correlation reference signal in the vibrator seismic exploration. Good application results were obtained in field experiments.

  12. Non-linear processes in thin titanium nitride transmission lines for parametric amplification (United States)

    Vissers, Michael; Gao, Jiansong; Chaudhuri, Suptarshi; Bockstiegel, Clint; Sandberg, Martin; Pappas, David P.


    Nitride superconductors, such as titanium nitride and niobium titanium nitride, are a non-linear, low dissipation medium at microwave frequencies. The lossless nonlinearity may be probed and utilized. Important applications include generation of higher harmonics, e.g. 3f, and a microwave version of the optical paramagnetic amplifier, i.e. the degenerate-pump case of four-photon mixing (FPM). An amplifier based on these principles should allow for very wide bandwidth, low noise (quantum limited) and high dynamic range devices. These measurements are performed via a single layer, 3 meter long TiN spiral and measured at temperatures below 100 mK. Initial results of the design, fabrication, testing, and impedance optimization of a titanium nitride based parametric amplifier are presented.

  13. Partial and total actuator faults accommodation for input-affine nonlinear process plants. (United States)

    Mihankhah, Amin; Salmasi, Farzad R; Salahshoor, Karim


    In this paper, a new fault-tolerant control system is proposed for input-affine nonlinear plants based on Model Reference Adaptive System (MRAS) structure. The proposed method has the capability to accommodate both partial and total actuator failures along with bounded external disturbances. In this methodology, the conventional MRAS control law is modified by augmenting two compensating terms. One of these terms is added to eliminate the nonlinear dynamic, while the other is reinforced to compensate the distractive effects of the total actuator faults and external disturbances. In addition, no Fault Detection and Diagnosis (FDD) unit is needed in the proposed method. Moreover, the control structure has good robustness capability against the parameter variation. The performance of this scheme is evaluated using a CSTR system and the results were satisfactory.

  14. Physiological processes non-linearly affect electrophysiological recordings during transcranial electric stimulation. (United States)

    Noury, Nima; Hipp, Joerg F; Siegel, Markus


    Transcranial electric stimulation (tES) is a promising tool to non-invasively manipulate neuronal activity in the human brain. Several studies have shown behavioral effects of tES, but stimulation artifacts complicate the simultaneous investigation of neural activity with EEG or MEG. Here, we first show for EEG and MEG, that contrary to previous assumptions, artifacts do not simply reflect stimulation currents, but that heartbeat and respiration non-linearly modulate stimulation artifacts. These modulations occur irrespective of the stimulation frequency, i.e. during both transcranial alternating and direct current stimulations (tACS and tDCS). Second, we show that, although at first sight previously employed artifact rejection methods may seem to remove artifacts, data are still contaminated by non-linear stimulation artifacts. Because of their complex nature and dependence on the subjects' physiological state, these artifacts are prone to be mistaken as neural entrainment. In sum, our results uncover non-linear tES artifacts, show that current techniques fail to fully remove them, and pave the way for new artifact rejection methods.

  15. Effects of high hydrostatic pressures on living cells: a consequence of the properties of macromolecules and macromolecule-associated water. (United States)

    Mentré, P; Hui Bon Hoa, G


    Sixty percent of the Earth's biomass is found in the sea, at depths greater than 1000 m, i.e., at hydrostatic pressures higher than 100 atm. Still more surprising is the fact that living cells can reversibly withstand pressure shifts of 1000 atm. One explanation lies in the properties of cellular water. Water forms a very thin film around macromolecules, with a heterogeneous structure that is an image of the heterogeneity of the macromolecular surface. The density of water in contact with macromolecules reflects the physical properties of their different domains. Therefore, any macromolecular shape variations involving the reorganization of water and concomitant density changes are sensitive to pressure (Le Chatelier's principle). Most of the pressure-induced changes to macromolecules are reversible up to 2000 atm. Both the effects of pressure shifts on living cells and the characteristics of pressure-adapted species are opening new perspectives on fundamental problems such as regulation and adaptation.

  16. Limitations of Feedback, Feedforward and IMC Controller for a First Order Non-Linear Process with Dead Time

    Directory of Open Access Journals (Sweden)

    Maruthai Suresh


    Full Text Available A nonlinear process, the heat exchanger whose parameters vary with respect to the process variable, is considered. The time constant and gain of the chosen process vary as a function of temperature. The limitations of the conventional feedback controller tuned using Ziegler-Nichols settings for the chosen process are brought out. The servo and regulatory responses through simulation and experimentation for various magnitudes of set-point changes and load changes at various operating points with the controller tuned only at a chosen nominal operating point are obtained and analyzed. Regulatory responses for output load changes are studied. The efficiency of feedforward controller and the effects of modeling error have been brought out. An IMC based system is presented to understand clearly how variations of system parameters affect the performance of the controller. The present work illustrates the effectiveness of Feedforward and IMC controller.

  17. Sub-terahertz resonance spectroscopy of biological macromolecules and cells (United States)

    Globus, Tatiana; Moyer, Aaron; Gelmont, Boris; Khromova, Tatyana; Sizov, Igor; Ferrance, Jerome


    Recently we introduced a Sub-THz spectroscopic system for characterizing vibrational resonance features from biological materials. This new, continuous-wave, frequency-domain spectroscopic sensor operates at room temperature between 315 and 480 GHz with spectral resolution of at least 1 GHz and utilizes the source and detector components from Virginia Diode, Inc. In this work we present experimental results and interpretation of spectroscopic signatures from bacterial cells and their biological macromolecule structural components. Transmission and absorption spectra of the bacterial protein thioredoxin, DNA and lyophilized cells of Escherichia coli (E. coli), as well as spores of Bacillus subtillis and B. atrophaeus have been characterized. Experimental results for biomolecules are compared with absorption spectra calculated using molecular dynamics simulation, and confirm the underlying physics for resonance spectroscopy based on interactions between THz radiation and vibrational modes or groups of modes of atomic motions. Such interactions result in multiple intense and narrow specific resonances in transmission/absorption spectra from nano-gram samples with spectral line widths as small as 3 GHz. The results of this study indicate diverse relaxation dynamic mechanisms relevant to sub-THz vibrational spectroscopy, including long-lasting processes. We demonstrate that high sensitivity in resolved specific absorption fingerprints provides conditions for reliable detection, identification and discrimination capability, to the level of strains of the same bacteria, and for monitoring interactions between biomaterials and reagents in near real-time. Additionally, it creates the basis for the development of new types of advanced biological sensors through integrating the developed system with a microfluidic platform for biomaterial samples.

  18. Insulin facilitates transport of macromolecules and nutrients to muscles

    DEFF Research Database (Denmark)

    Christensen, N J; Hilsted, J


    , systolic blood pressure and plasma noradrenaline. These changes were absent or attenuated in diabetic patients (without neuropathy) after an oral glucose load. These responses were normalized by insulin infusion. Our results suggest that insulin facilitates the transfer of macromolecules and nutrients from...


    NARCIS (Netherlands)



    Macromolecules can be used to derive evolutionary relationships. This discovery has led to the development of a new discipline in biology, molecular evolution. A brief survey of several aspects of this new field of investigation is presented, together with references to historical and more recent li

  20. Crystallogenesis of biological macromolecules. Biological, microgravity and other physicochemical aspects

    NARCIS (Netherlands)

    Giege, R; Drenth, J; Ducruix, A; McPherson, A; Saenger, W


    After an historical introduction and justification of the importance of proteins (as well as other macromolecules or macromolecular assemblies of biological origin) in modern biology but also in physics, this review presents the state of the field of macromolecular crystallogenesis. The basic questi

  1. Reduced adipose tissue lymphatic drainage of macromolecules in obese subjects

    DEFF Research Database (Denmark)

    Arngrim, N; Simonsen, L; Holst, Jens Juul


    The aim of this study was to investigate subcutaneous adipose tissue lymphatic drainage (ATLD) of macromolecules in lean and obese subjects and, furthermore, to evaluate whether ATLD may change in parallel with adipose tissue blood flow. Lean and obese male subjects were studied before and after ...... online publication, 3 July 2012; doi:10.1038/ijo.2012.98....

  2. Chaperoning roles of macromolecules interacting with proteins in vivo. (United States)

    Choi, Seong Il; Lim, Keo-Heun; Seong, Baik L


    The principles obtained from studies on molecular chaperones have provided explanations for the assisted protein folding in vivo. However, the majority of proteins can fold without the assistance of the known molecular chaperones, and little attention has been paid to the potential chaperoning roles of other macromolecules. During protein biogenesis and folding, newly synthesized polypeptide chains interact with a variety of macromolecules, including ribosomes, RNAs, cytoskeleton, lipid bilayer, proteolytic system, etc. In general, the hydrophobic interactions between molecular chaperones and their substrates have been widely believed to be mainly responsible for the substrate stabilization against aggregation. Emerging evidence now indicates that other features of macromolecules such as their surface charges, probably resulting in electrostatic repulsions, and steric hindrance, could play a key role in the stabilization of their linked proteins against aggregation. Such stabilizing mechanisms are expected to give new insights into our understanding of the chaperoning functions for de novo protein folding. In this review, we will discuss the possible chaperoning roles of these macromolecules in de novo folding, based on their charge and steric features.

  3. Dermal and transdermal delivery of pharmaceutically relevant macromolecules. (United States)

    Münch, S; Wohlrab, J; Neubert, R H H


    The skin offers an attractive way for dermal and transdermal drug delivery that is why the drug still needs certain qualities to transcend the outermost layer of the skin, the stratum corneum. The requirements are: drugs with a maximum molecular weight of 1kDa, high lipophilicity and a certain polarity. This would restrict the use of a transdermal delivery of macromolecules, which would make the drug more effective in therapeutic administration. Various studies have shown that macromolecules without support do not penetrate the human skin. This effect can be achieved using physical and chemical methods, as well as biological peptides. The most popular physical method is the use of microneedles to create micropores in the skin and release the active agent in different sections. But also, other methods have been tested. Microjets, lasers, electroporation, sonophoresis and iontophoresis are also promising methods to successfully deliver dermal and transdermal macromolecules. Additionally, there are different penetration enhancer groups and biological peptides, which are also considered to be interesting approaches of enabling macromolecules to travel along the skin. All these methods will be described and evaluated in this review article. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Crystallization of biological macromolecules in a reduced gravity environment (United States)

    Meehan, E. J., Jr.


    A battery of micro techniques were developed which allow the screening of a large number of conditions using only a small amount of the macromolecule. The need to develop methodologies for growing large crystals required for neutron diffraction studies is discussed.

  5. Interactions between adsorbed macromolecules : measurements on emulsions and liquid films

    NARCIS (Netherlands)

    Vliet, van T.


    The aim of this study was to gain more insight into the factors, determining the inter- and intramolecular interactions between adsorbed macromolecules. To that end several experimental and theoretical approaches were followed, using well-defined systems. It was shown that these interactions could c

  6. Synthesis of main-chain metal carbonyl organometallic macromolecules (MCMCOMs). (United States)

    Cao, Kai; Murshid, Nimer; Wang, Xiaosong


    Synthesis of main-chain metal carbonyl organometallic macromolecules (MCMCOMs) is difficult, mainly due to the instability of metal carbonyl complexes. Despite its challenge a number of MCMCOMs has been prepared by strategically using organometallic, organic, and polymer synthetic chemistry. Main contributions to this research field were reported by the groups of Tyler, Pannell, and Wang and are briefly summarized in this article.

  7. Chaperoning Roles of Macromolecules Interacting with Proteins in Vivo

    Directory of Open Access Journals (Sweden)

    Baik L. Seong


    Full Text Available The principles obtained from studies on molecular chaperones have provided explanations for the assisted protein folding in vivo. However, the majority of proteins can fold without the assistance of the known molecular chaperones, and little attention has been paid to the potential chaperoning roles of other macromolecules. During protein biogenesis and folding, newly synthesized polypeptide chains interact with a variety of macromolecules, including ribosomes, RNAs, cytoskeleton, lipid bilayer, proteolytic system, etc. In general, the hydrophobic interactions between molecular chaperones and their substrates have been widely believed to be mainly responsible for the substrate stabilization against aggregation. Emerging evidence now indicates that other features of macromolecules such as their surface charges, probably resulting in electrostatic repulsions, and steric hindrance, could play a key role in the stabilization of their linked proteins against aggregation. Such stabilizing mechanisms are expected to give new insights into our understanding of the chaperoning functions for de novo protein folding. In this review, we will discuss the possible chaperoning roles of these macromolecules in de novo folding, based on their charge and steric features.

  8. EGS Richardson AGU Chapman NVAG3 Conference: Nonlinear Variability in Geophysics: scaling and multifractal processes (United States)

    Schertzer, D.; Lovejoy, S.

    1. The conference The third conference on "Nonlinear VAriability in Geophysics: scaling and multifractal processes" (NVAG 3) was held in Cargese, Corsica, Sept. 10-17, 1993. NVAG3 was joint American Geophysical Union Chapman and European Geophysical Society Richardson Memorial conference, the first specialist conference jointly sponsored by the two organizations. It followed NVAG1 (Montreal, Aug. 1986), NVAG2 (Paris, June 1988; Schertzer and Lovejoy, 1991), five consecutive annual sessions at EGS general assemblies and two consecutive spring AGU meeting sessions. As with the other conferences and workshops mentioned above, the aim was to develop confrontation between theories and experiments on scaling/multifractal behaviour of geophysical fields. Subjects covered included climate, clouds, earthquakes, atmospheric and ocean dynamics, tectonics, precipitation, hydrology, the solar cycle and volcanoes. Areas of focus included new methods of data analysis (especially those used for the reliable estimation of multifractal and scaling exponents), as well as their application to rapidly growing data bases from in situ networks and remote sensing. The corresponding modelling, prediction and estimation techniques were also emphasized as were the current debates about stochastic and deterministic dynamics, fractal geometry and multifractals, self-organized criticality and multifractal fields, each of which was the subject of a specific general discussion. The conference started with a one day short course of multifractals featuring four lectures on a) Fundamentals of multifractals: dimension, codimensions, codimension formalism, b) Multifractal estimation techniques: (PDMS, DTM), c) Numerical simulations, Generalized Scale Invariance analysis, d) Advanced multifractals, singular statistics, phase transitions, self-organized criticality and Lie cascades (given by D. Schertzer and S. Lovejoy, detailed course notes were sent to participants shortly after the conference). This

  9. EGS Richardson AGU Chapman NVAG3 Conference: Nonlinear Variability in Geophysics: scaling and multifractal processes

    Directory of Open Access Journals (Sweden)

    D. Schertzer


    Full Text Available 1. The conference The third conference on "Nonlinear VAriability in Geophysics: scaling and multifractal processes" (NVAG 3 was held in Cargese, Corsica, Sept. 10-17, 1993. NVAG3 was joint American Geophysical Union Chapman and European Geophysical Society Richardson Memorial conference, the first specialist conference jointly sponsored by the two organizations. It followed NVAG1 (Montreal, Aug. 1986, NVAG2 (Paris, June 1988; Schertzer and Lovejoy, 1991, five consecutive annual sessions at EGS general assemblies and two consecutive spring AGU meeting sessions. As with the other conferences and workshops mentioned above, the aim was to develop confrontation between theories and experiments on scaling/multifractal behaviour of geophysical fields. Subjects covered included climate, clouds, earthquakes, atmospheric and ocean dynamics, tectonics, precipitation, hydrology, the solar cycle and volcanoes. Areas of focus included new methods of data analysis (especially those used for the reliable estimation of multifractal and scaling exponents, as well as their application to rapidly growing data bases from in situ networks and remote sensing. The corresponding modelling, prediction and estimation techniques were also emphasized as were the current debates about stochastic and deterministic dynamics, fractal geometry and multifractals, self-organized criticality and multifractal fields, each of which was the subject of a specific general discussion. The conference started with a one day short course of multifractals featuring four lectures on a Fundamentals of multifractals: dimension, codimensions, codimension formalism, b Multifractal estimation techniques: (PDMS, DTM, c Numerical simulations, Generalized Scale Invariance analysis, d Advanced multifractals, singular statistics, phase transitions, self-organized criticality and Lie cascades (given by D. Schertzer and S. Lovejoy, detailed course notes were sent to participants shortly after the

  10. Effects of noninstantaneous nonlinear processes on photon-pair generation by spontaneous four-wave mixing

    DEFF Research Database (Denmark)

    Koefoed, Jacob Gade; Christensen, Jesper Bjerge; Rottwitt, Karsten


    We present a general model, based on a Hamiltonian approach, for the joint quantum state of photon pairs generated through pulsed spontaneous four-wave mixing, including nonlinear phase modulation and a finite material response time. For the case of a silica fiber, it is found that the pair......-dependent change in quantum-mechanical purity may be observed in silica. This shows that Raman scattering not only introduces noise, but can also drastically change the spectral correlations in photon pairs when pumped with short pulses....

  11. Nonlinear Deformation Processes and Damage Modes of Super Carbon Nanotubes with Armchair-Armchair Topology

    Institute of Scientific and Technical Information of China (English)

    CHEN Yu-Li; LIU Bin; YIN Ya-Jun; HUANG Yong-Gang; HWUANG Keh-Chih


    The tensile deformations and fractures of super carbon nanotubes (SCNTs) with armchair-armchair topology are investigated by using the atomic-scale finite element method. SCNTs generated from carbon nanotubes (CNTs) with different characteristic aspect ratios are found to have different nonlinear behaviours under uniaxiai tensions. Specifically, an SCNT with higher aspect ratio has three distinct stages: rotation, stretch and rupture, while an SCNT with lower aspect ratio has only two stages. This information may compensate for previous work and enrich our knowledge about Y-branched CNTs and SCNTs.

  12. Nonlinear model-based control of the Czochralski process III: Proper choice of manipulated variables and controller parameter scheduling (United States)

    Neubert, M.; Winkler, J.


    This contribution continues an article series [1,2] about the nonlinear model-based control of the Czochralski crystal growth process. The key idea of the presented approach is to use a sophisticated combination of nonlinear model-based and conventional (linear) PI controllers for tracking of both, crystal radius and growth rate. Using heater power and pulling speed as manipulated variables several controller structures are possible. The present part tries to systematize the properties of the materials to be grown in order to get unambiguous decision criteria for a most profitable choice of the controller structure. For this purpose a material specific constant M called interface mobility and a more process specific constant S called system response number are introduced. While the first one summarizes important material properties like thermal conductivity and latent heat the latter one characterizes the process by evaluating the average axial thermal gradients at the phase boundary and the actual growth rate at which the crystal is grown. Furthermore these characteristic numbers are useful for establishing a scheduling strategy for the PI controller parameters in order to improve the controller performance. Finally, both numbers give a better understanding of the general thermal system dynamics of the Czochralski technique.

  13. Effects of injection timing on nonlinear dynamics of the combustion process in the lean-burn premixed natural gas engine (United States)

    Ding, Shun-Liang; Song, En-Zhe; Yang, Li-Ping; Yao, Chong; Ma, Xiu-Zhen


    The nonlinear dynamics of the combustion process in the lean-burn premixed natural gas engine are studied in this paper. Based on nonlinear dynamic theory, the complexity of the combustion process is analyzed under different injection timing conditions. The phase spaces are reconstructed for the experimentally obtained in-cylinder pressure real-time series and the return maps are plotted for the IMEP time series. The results of phase space reconstruction manifest that the attractors are limited to the finite range in the reconstructed phase space. The attractors have a folded and twist geometry structure. The attractors under medium injection timing conditions are looser and more complex. The return maps indicate the coexistence of the stochastic and deterministic components in the patterns combustion process. With the injection timing increasing, there are both a transition from stochastic to deterministic and a transition from deterministic to stochastic, forming the region of deterministic behavior. The largest Lyapunov exponents (LLE) for in-cylinder pressure time series are calculated and the coefficients of variations (COV) of IMEP are also analyzed. The results express that the LLE values are positive. There are a "steep increase" and a "steep decrease" for the LLE and COV values as the injection timing increasing.

  14. Light-Switchable Azobenzene-Containing Macromolecules: From UV to Near Infrared. (United States)

    Weis, Philipp; Wu, Si


    Azobenzene-containing macromolecules (azo-macromolecules) such as azobenzene-containing polymers (azopolymers) and azobenzene-functionalized biomacromolecules are photoswitchable macromolecules. Trans-to-cis photoisomerization in conventional azo-macromolecules is induced by ultraviolet (UV) light. However, UV light cannot penetrate deeply into issue and has a very small fraction in sunlight. Therefore, conventional azo-macromolecules are problematic for biomedical and solar-energy-related applications. In this Feature Article, the strategies for constructing visible and near-infrared (NIR) light-responsive azo-macromolecules are reviewed, and the potential applications of visible- and NIR-light-responsive azo-macromolecules in biomedicine and solar energy conversion are highlighted. The remaining challenges in the field of photoswitchable azo-macromolecules are discussed. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Surface Characteristics and Adhesion Behavior of Escherichia coli O157:H7: Role of Extracellular Macromolecules (United States)

    Surface macromolecule cleavage experiments were conducted on enterohaemorrhagic Escherichia coli O157:H7 cells to investigate the influence of these macromolecules on cell surface properties. Electrophoretic mobility, hydrophobicity, and titration experiments were carried out on proteinase K treate...

  16. Vesicular uptake of macromolecules by human placental amniotic epithelial cells. (United States)

    Sharshiner, Rita; Brace, Robert A; Cheung, Cecilia Y


    Studies in animal models have shown that unidirectional vesicular transport of amniotic fluid across the amnion plays a primary role in regulating amniotic fluid volume. Our objective was to explore vesicle type, vesicular uptake and intracellular distribution of vesicles in human amnion cells using high- and super-resolution fluorescence microscopy. Placental amnion was obtained at cesarean section and amnion cells were prepared and cultured. At 20%-50% confluence, the cells were incubated with fluorophore conjugated macromolecules for 1-30 min at 22 °C or 37 °C. Fluorophore labeled macromolecules were selected as markers of receptor-mediated caveolar and clathrin-coated vesicular uptake as well as non-specific endocytosis. After fluorophore treatment, the cells were fixed, imaged and vesicles counted using Imaris(®) software. Vesicular uptake displayed first order saturation kinetics with half saturation times averaging 1.3 min at 37 °C compared to 4.9 min at 22 °C, with non-specific endocytotic uptake being more rapid at both temperatures. There was extensive cell-to-cell variability in uptake rate. Under super-resolution microscopy, the pattern of intracellular spatial distribution was distinct for each macromolecule. Co-localization of fluorescently labeled macromolecules was very low at vesicular dimensions. In human placental amnion cells, 1) vesicular uptake of macromolecules is rapid, consistent with the concept that vesicular transcytosis across the amnion plays a role in the regulation of amniotic fluid volume; 2) uptake is temperature dependent and variable among individual cells; 3) the unique intracellular distributions suggest distinct functions for each vesicle type; 4) non-receptor mediated vesicular uptake may be a primary vesicular uptake mechanism. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Finite-dimensional constrained fuzzy control for a class of nonlinear distributed process systems. (United States)

    Wu, Huai-Ning; Li, Han-Xiong


    This correspondence studies the problem of finite-dimensional constrained fuzzy control for a class of systems described by nonlinear parabolic partial differential equations (PDEs). Initially, Galerkin's method is applied to the PDE system to derive a nonlinear ordinary differential equation (ODE) system that accurately describes the dynamics of the dominant (slow) modes of the PDE system. Subsequently, a systematic modeling procedure is given to construct exactly a Takagi-Sugeno (T-S) fuzzy model for the finite-dimensional ODE system under state constraints. Then, based on the T-S fuzzy model, a sufficient condition for the existence of a stabilizing fuzzy controller is derived, which guarantees that the state constraints are satisfied and provides an upper bound on the quadratic performance function for the finite-dimensional slow system. The resulting fuzzy controllers can also guarantee the exponential stability of the closed-loop PDE system. Moreover, a local optimization algorithm based on the linear matrix inequalities is proposed to compute the feedback gain matrices of a suboptimal fuzzy controller in the sense of minimizing the quadratic performance bound. Finally, the proposed design method is applied to the control of the temperature profile of a catalytic rod.

  18. Rapid prediction method for nonlinear expansion process of medical vascular stent

    Institute of Scientific and Technical Information of China (English)


    A neural network model with high nonlinear recognition capability was constructed to describe the relationship between the deformation impact factors and the deformation results of vascular stent.Then,using the weighted correction method with the attached momentum term,the network training algorithm was optimized by introducing learning factor η and momentum factor ψ,so the speed of the network training and the system robustness were enhanced.The network was trained by some practi-cal cases,and the statistical hypothesis validation was made for the predictive errors.It was shown that the average difference between the intelligent predictive result of vascular stent deformation neu-ral network and the nonlinear finite element analysis result was less than 0.03%,and the trained net-work could perfectly predict the vascular stent deformation.Further more,the rapid evaluation tool for the vascular stent mechanics performance was established using the Pro/Toolkit and the intelligent neural network predictive model of vascular stent expansion.The proposed tool system with strong practicality and high efficiency can significantly shorten the product development cycle of vascular stent.

  19. Nonlinear Systems. (United States)

    Seider, Warren D.; Ungar, Lyle H.


    Describes a course in nonlinear mathematics courses offered at the University of Pennsylvania which provides an opportunity for students to examine the complex solution spaces that chemical engineers encounter. Topics include modeling many chemical processes, especially those involving reaction and diffusion, auto catalytic reactions, phase…

  20. Macromolecule Mediated Transport and Retention of Escherichia coli O157:H7 in Saturated Porous Media (United States)

    The role of extracellular macromolecules on Escherichia coli O157:H7 transport and retention was investigated in saturated porous media. To compare the relative transport and retention of E. coli cells that are macromolecule rich and deficient, macromolecules were partially cleaved using a proteolyt...

  1. Measuring Equilibrium Binding Affinity of Biological Macromolecules in Solution by Thermophoresis (United States)


    Approved for Public Release; Distribution Unlimited Final Report: Measuring Equilibrium Binding Affinity of Biological Macromolecules in Solution by...Equilibrium Binding Affinity of Biological Macromolecules in Solution by Thermophoresis Report Title The primary research focus of the San Diego State...equilibrium binding affinities of biological macromolecules in solution. This qauntitative information plays a vital role in supporting the static

  2. Nonlinear Memory Capacity of Parallel Time-Delay Reservoir Computers in the Processing of Multidimensional Signals. (United States)

    Grigoryeva, Lyudmila; Henriques, Julie; Larger, Laurent; Ortega, Juan-Pablo


    This letter addresses the reservoir design problem in the context of delay-based reservoir computers for multidimensional input signals, parallel architectures, and real-time multitasking. First, an approximating reservoir model is presented in those frameworks that provides an explicit functional link between the reservoir architecture and its performance in the execution of a specific task. Second, the inference properties of the ridge regression estimator in the multivariate context are used to assess the impact of finite sample training on the decrease of the reservoir capacity. Finally, an empirical study is conducted that shows the adequacy of the theoretical results with the empirical performances exhibited by various reservoir architectures in the execution of several nonlinear tasks with multidimensional inputs. Our results confirm the robustness properties of the parallel reservoir architecture with respect to task misspecification and parameter choice already documented in the literature.

  3. THz Generation by Optical Rectification and Competition with Other Nonlinear Processes

    Institute of Scientific and Technical Information of China (English)

    ZHAO Zhen-Yu; HAMEAU Sophie; TIGNON Jér(o)me


    We present a study of the competition between tera-hertz (THz) generation by optical rectification in (110)Zn Te crystals,two-photon absorption,second harmonic generation and flee-carrier absorption.The two-photon nonlinear absorption coefficient,second harmonic generation efficiency and flee-carrier absorption coefficient in the THz range are measured independently.The incident pump field is shown to be depleted by two-photon absorption and the THz radiation is shown to be reduced,upon focusing,by free-carrier absorption.The reduction of the generated THz radiation upon tight focusing is explained,provided that one also takes into account diffraction effects from the sub-wavelength THz source.

  4. Modelling and prediction of complex non-linear processes by using Pareto multi-objective genetic programming (United States)

    Jamali, A.; Khaleghi, E.; Gholaminezhad, I.; Nariman-zadeh, N.


    In this paper, a new multi-objective genetic programming (GP) with a diversity preserving mechanism and a real number alteration operator is presented and successfully used for Pareto optimal modelling of some complex non-linear systems using some input-output data. In this study, two different input-output data-sets of a non-linear mathematical model and of an explosive cutting process are considered separately in three-objective optimisation processes. The pertinent conflicting objective functions that have been considered for such Pareto optimisations are namely, training error (TE), prediction error (PE), and the length of tree (complexity of the network) (TL) of the GP models. Such three-objective optimisation implementations leads to some non-dominated choices of GP-type models for both cases representing the trade-offs among those objective functions. Therefore, optimal Pareto fronts of such GP models exhibit the trade-off among the corresponding conflicting objectives and, thus, provide different non-dominated optimal choices of GP-type models. Moreover, the results show that no significant optimality in TE and PE may occur when the TL of the corresponding GP model exceeds some values.

  5. Science-Grade Observing Systems as Process Observatories: Mapping and Understanding Nonlinearity and Multiscale Memory with Models and Observations (United States)

    Barros, A. P.; Wilson, A. M.; Miller, D. K.; Tao, J.; Genereux, D. P.; Prat, O.; Petersen, W. A.; Brunsell, N. A.; Petters, M. D.; Duan, Y.


    Using the planet as a study domain and collecting observations over unprecedented ranges of spatial and temporal scales, NASA's EOS (Earth Observing System) program was an agent of transformational change in Earth Sciences over the last thirty years. The remarkable space-time organization and variability of atmospheric and terrestrial moist processes that emerged from the analysis of comprehensive satellite observations provided much impetus to expand the scope of land-atmosphere interaction studies in Hydrology and Hydrometeorology. Consequently, input and output terms in the mass and energy balance equations evolved from being treated as fluxes that can be used as boundary conditions, or forcing, to being viewed as dynamic processes of a coupled system interacting at multiple scales. Measurements of states or fluxes are most useful if together they map, reveal and/or constrain the underlying physical processes and their interactions. This can only be accomplished through an integrated observing system designed to capture the coupled physics, including nonlinear feedbacks and tipping points. Here, we first review and synthesize lessons learned from hydrometeorology studies in the Southern Appalachians and in the Southern Great Plains using both ground-based and satellite observations, physical models and data-assimilation systems. We will specifically focus on mapping and understanding nonlinearity and multiscale memory of rainfall-runoff processes in mountainous regions. It will be shown that beyond technical rigor, variety, quantity and duration of measurements, the utility of observing systems is determined by their interpretive value in the context of physical models to describe the linkages among different observations. Second, we propose a framework for designing science-grade and science-minded process-oriented integrated observing and modeling platforms for hydrometeorological studies.

  6. Behavior of macromolecules in adsorbed layers

    Institute of Scientific and Technical Information of China (English)

    牟伯中[1; 姚恒申[2; 罗平亚[3


    A model for describing the behavior ot macromoiecuies in aosoroea layers is developed by introducing a concept of distribution density of layer thickness U based on stochastic process and probabilistic statistics. The molecular behavior of layers adsorbed on clay particle surfaces is discussed; the random distribution and its statistics of the layer thickness are given by incorporating experimental results with an ionic polyelectrolyte with the molecular weight of 1.08×106and chain charged density of 0.254.

  7. Development of PUNDA (Parametric Universal Nonlinear Dynamics Approximator) Models for Self-Validating Knowledge-Guided Modelling of Nonlinear Processes in Particle Accelerators \\& Industry

    Energy Technology Data Exchange (ETDEWEB)

    Sayyar-Rodsari, Bijan; Schweiger, Carl; Hartman, Eric


    The difficult problems being tackled in the accelerator community are those that are nonlinear, substantially unmodeled, and vary over time. Such problems are ideal candidates for model-based optimization and control if representative models of the problem can be developed that capture the necessary mathematical relations and remain valid throughout the operation region of the system, and through variations in system dynamics. The goal of this proposal is to develop the methodology and the algorithms for building high-fidelity mathematical representations of complex nonlinear systems via constrained training of combined first-principles and neural network models.

  8. Nonlinear PDEs



    From the Back Cover: The emphasis throughout the present volume is on the practical application of theoretical mathematical models helping to unravel the underlying mechanisms involved in processes from mathematical physics and biosciences. It has been conceived as a unique collection of abstract methods dealing especially with nonlinear partial differential equations (either stationary or evolutionary) that are applied to understand concrete processes involving some important applications re...

  9. State and parameter estimation based on a nonlinear filter applied to an industrial process control of ethanol production

    Directory of Open Access Journals (Sweden)

    Meleiro L.A.C.


    Full Text Available Most advanced computer-aided control applications rely on good dynamics process models. The performance of the control system depends on the accuracy of the model used. Typically, such models are developed by conducting off-line identification experiments on the process. These experiments for identification often result in input-output data with small output signal-to-noise ratio, and using these data results in inaccurate model parameter estimates [1]. In this work, a multivariable adaptive self-tuning controller (STC was developed for a biotechnological process application. Due to the difficulties involving the measurements or the excessive amount of variables normally found in industrial process, it is proposed to develop "soft-sensors" which are based fundamentally on artificial neural networks (ANN. A second approach proposed was set in hybrid models, results of the association of deterministic models (which incorporates the available prior knowledge about the process being modeled with artificial neural networks. In this case, kinetic parameters - which are very hard to be accurately determined in real time industrial plants operation - were obtained using ANN predictions. These methods are especially suitable for the identification of time-varying and nonlinear models. This advanced control strategy was applied to a fermentation process to produce ethyl alcohol (ethanol in industrial scale. The reaction rate considered for substratum consumption, cells and ethanol productions are validated with industrial data for typical operating conditions. The results obtained show that the proposed procedure in this work has a great potential for application.

  10. Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity (United States)

    Duff,, Michael R.; Grubbs, Jordan; Howell, Elizabeth E.


    Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. In biological sciences, macromolecular interactions are essential in understanding the machinery of the cell. Experimental conditions, such as buffer and temperature, can be tailored to the particular binding system being studied. However, careful planning is needed since certain ligand and macromolecule concentration ranges are necessary to obtain useful data. Concentrations of the macromolecule and ligand need to be accurately determined for reliable results. Care also needs to be taken when preparing the samples as impurities can significantly affect the experiment. When ITC experiments, along with controls, are performed properly, useful binding information, such as the stoichiometry, affinity and enthalpy, are obtained. By running additional experiments under different buffer or temperature conditions, more detailed information can be obtained about the system. A protocol for the basic setup of an ITC experiment is given. PMID:21931288

  11. High and low thermal conductivity of amorphous macromolecules (United States)

    Xie, Xu; Yang, Kexin; Li, Dongyao; Tsai, Tsung-Han; Shin, Jungwoo; Braun, Paul V.; Cahill, David G.


    We measure the thermal conductivity, heat capacity and sound velocity of thin films of five polymers, nine polymer salts, and four caged molecules to advance the fundamental understanding of the lower and upper limits to heat conduction in amorphous macromolecules. The thermal conductivities vary by more than one order of magnitude, from 0.06 W m-1K-1 for [6,6]-phenyl-C71-butyric acid methyl ester to 0.67 W m-1K-1 for poly(vinylphosphonic acid calcium salt). Minimum thermal conductivity calculated from the measured sound velocity and effective atomic density is in good agreement with the thermal conductivity of macromolecules with various molecular structures and intermolecular bonding strength.

  12. Non-linear dynamics of inlet film thickness during unsteady rolling process (United States)

    Fu, Kuo; Zang, Yong; Gao, Zhiying; Qin, Qin; Wu, Diping


    The inlet film thickness directly affects film and stress distribution of rolling interfaces. Unsteady factors, such as unsteady back tension, may disturb the inlet film thickness. However, the current models of unsteady inlet film thickness lack unsteady disturbance factors and do not take surface topography into consideration. In this paper, based on the hydrodynamic analysis of inlet zone an unsteady rolling film model which concerns the direction of surface topography is built up. Considering the small fluctuation of inlet angle, absolute reduction, reduction ratio, inlet strip thickness and roll radius as the input variables and the fluctuation of inlet film thickness as the output variable, the non-linear relationship between the input and output is discussed. The discussion results show that there is 180° phase difference between the inlet film thickness and the input variables, such as the fluctuant absolute reduction, the fluctuant reduction ratio and non-uniform inlet strip thickness, but there is no phase difference between unsteady roll radius and the output. The inlet angle, the steady roll radius and the direction of surface topography have significant influence on the fluctuant amplitude of unsteady inlet film thickness. This study proposes an analysis method for unsteady inlet film thickness which takes surface topography and new disturbance factors into consideration.

  13. Localized surface plasmons modulated nonlinear optical processes in metal film-coupled and upconversion nanocrystals-coated nanoparticles (Conference Presentation) (United States)

    Lei, Dangyuan


    In the first part of this talk, I will show our experimental investigation on the linear and nonlinear optical properties of metal film-coupled nanosphere monomers and dimers both with nanometric gaps. We have developed a new methodology - polarization resolved spectral decomposition and color decoding to "visualizing" unambiguously the spectral and radiation properties of the complex plasmonic gap modes in these hybrid nanostructures. Single-particle spectroscopic measurements indicate that these hybrid nanostructures can simultaneously enhance several nonlinear optical processes, such as second harmonic generation, two-photon absorption induced luminescence, and hyper-Raman scattering. In the second part, I will show how the polarization state of the emissions from sub-10 nm upconversion nanocrystals (UCNCs) can be modulated when they form a hybrid complex with a gold nanorod (GNR). Our single-particle scattering experiments expose how an interplay between excitation polarization and GNR orientation gives rise to an extraordinary polarized nature of the upconversion emissions from an individual hybrid nanostructure. We support our results by numerical simulations and, using Förster resonance energy transfer theory, we uncover how an overlap between the UCNC emission and GNR extinction bands as well as the mutual orientation between emission and plasmonic dipoles jointly determine the polarization state of the UC emissions.

  14. Nonlinear optical and multiphoton processes for in situ manipulation and conversion of photons: applications to energy and healthcare (Conference Presentation) (United States)

    Prasad, Paras N.


    Chiral control of nonlinear optical functions holds a great promise for a wide range of applications including optical signal processing, bio-sensing and chiral bio-imaging. In chiral polyfluorene thin films, we demonstrated extremely large chiral nonlinearity. The physics of manipulating excitation dynamics for photon transformation will be discussed, along with nanochemistry control of upconversion in hierarchically built organic chromophore coupled-core-multiple shell nanostructures which enable introduce new, organic-inorganic energy transfer routes for broadband light harvesting and increased upconversion efficiency via multistep cascaded energy transfer. We are pursuing the applications of photon conversion technology in IR harvesting for photovoltaics, high contrast bioimaging, photoacoustic imaging, photodynamic therapy, and optogenetics. An important application is in Brain research and Neurophotonics for functional mapping and modulation of brain activities. Another new direction pursued is magnetic field control of light in in a chiral polymer nanocomposite to achieve large magneto-optic coefficient which can enable sensing of extremely weak magnetic field due to brain waves. Finally, we will consider the thought provoking concept of utilizing photons to quantify, through magneto-optics, and augment - through nanoptogenetics, the cognitive states, thus paving the path way to a quantified human paradigm.

  15. In-Fiber Subpicosecond Pulse Shaping for Nonlinear Optical Telecommunication Data Processing at 640 Gbit/s

    Directory of Open Access Journals (Sweden)

    J. Azaña


    Full Text Available We review recent work on all-fiber (long-period fiber grating devices for optical pulse shaping, particularly flat-top pulse generation, down to the subpicosecond range and their application for nonlinear switching (demultiplexing of optical time-division multiplexed (OTDM data signals in fiber-optic telecommunication links operating up to 640 Gbit/s. Experiments are presented demonstrating error-free 640-to-10 Gbit/s demultiplexing of the 64 tributary channels using the generated flat-top pulses for temporal gating in a Kerr-effect-based nonlinear optical loop mirror. The use of flat-top pulses has critical benefits in the demultiplexing process, including a significantly increased timing-jitter tolerance (up to ~500 fs, i.e., 30% of the bit period and the associated improvement in the bit-error-rate performance (e.g., with a sensitivity increase of up to ~13 dB as compared with the use of Gaussian-like gating pulses. Long-period fiber grating pulse shapers with reduced polarization dependence are fabricated and successfully used for polarization-independent 640-to-10 Gbit/s demultiplexing experiments.

  16. Synthesis, Characterisation and Application of 68Ga-labelled Macromolecules


    Velikyan, Irina


    The positron emitting radionuclide 68Ga (T1/2 = 68 min) might become of practical interest for clinical positron emission tomography (PET). The metallic cation, 68Ga(III), is suitable for complexation with chelators, either naked or conjugated with biological macromolecules. Such labelling procedures require pure and concentrated preparations of 68Ga(III), which cannot be sufficiently fulfilled by the presently available 68Ge/68Ga generator eluate. This thesis presents methods to increase the...

  17. Fault diagnosis of nonlinear and large-scale processes using novel modified kernel Fisher discriminant analysis approach (United States)

    Shi, Huaitao; Liu, Jianchang; Wu, Yuhou; Zhang, Ke; Zhang, Lixiu; Xue, Peng


    It is pretty significant for fault diagnosis timely and accurately to improve the dependability of industrial processes. In this study, fault diagnosis of nonlinear and large-scale processes by variable-weighted kernel Fisher discriminant analysis (KFDA) based on improved biogeography-based optimisation (IBBO) is proposed, referred to as IBBO-KFDA, where IBBO is used to determine the parameters of variable-weighted KFDA, and variable-weighted KFDA is used to solve the multi-classification overlapping problem. The main contributions of this work are four-fold to further improve the performance of KFDA for fault diagnosis. First, a nonlinear fault diagnosis approach with variable-weighted KFDA is developed for maximising separation between the overlapping fault samples. Second, kernel parameters and features selection of variable-weighted KFDA are simultaneously optimised using IBBO. Finally, a single fitness function that combines erroneous diagnosis rate with feature cost is created, a novel mixed kernel function is introduced to improve the classification capability in the feature space and diagnosis accuracy of the IBBO-KFDA, and serves as the target function in the optimisation problem. Moreover, an IBBO approach is developed to obtain the better quality of solution and faster convergence speed. On the one hand, the proposed IBBO-KFDA method is first used on Tennessee Eastman process benchmark data sets to validate the feasibility and efficiency. On the other hand, IBBO-KFDA is applied to diagnose faults of automation gauge control system. Simulation results demonstrate that IBBO-KFDA can obtain better kernel parameters and feature vectors with a lower computing cost, higher diagnosis accuracy and a better real-time capacity.


    Directory of Open Access Journals (Sweden)

    Jie Li


    Full Text Available The molecular basis of life rests on the activity of biological macromolecules, mostly nucleic acids and proteins. A perhaps surprising finding that crystallized over the last handful of decades is that geometric reasoning plays a major role in our attempt to understand these activities. In this paper, we address this connection between geometry and biology, focusing on methods for measuring and characterizing the shapes of macromolecules. We briefly review existing numerical and analytical approaches that solve these problems. We cover in more details our own work in this field, focusing on the alpha shape theory as it provides a unifying mathematical framework that enable the analytical calculations of the surface area and volume of a macromolecule represented as a union of balls, the detection of pockets and cavities in the molecule, and the quantification of contacts between the atomic balls. We have shown that each of these quantities can be related to physical properties of the molecule under study and ultimately provides insight on its activity. We conclude with a brief description of new challenges for the alpha shape theory in modern structural biology.

  19. Localization of intracrystalline organic macromolecules in mollusk shells (United States)

    Suzuki, Michio; Okumura, Taiga; Nagasawa, Hiromichi; Kogure, Toshihiro


    As a crucial first step for understanding the organic-inorganic interaction in biomineralization of mollscan shells, localization of the intracrystalline organic macromolecules in biogenic calcium carbonate crystals of the nacreous, prismatic ( Pinctada fucata) and foliated ( Cellana toreuma) microstructures were investigated using Fresnel contrast analysis in a transmission electron microscope. Spherular Fresnel contrasts in the crystals correspond to organic substances, which was confirmed by the detection of 1s→π * (CC) transition peak at 284 eV in electron energy loss spectroscopy. Nano-sized (5-10 nm) spherules in the aragonite tablets constituting the nacreous layer of P. fucata specifically concentrate in the vicinity of the interlamellar membrane between the aragonite tablets. The dominant sizes of the organic macromolecules extracted by dissolving the aragonite tablets in the nacreous layer of P. fucata were estimated using the gel-filtration analysis to be roughly 10 and 4 nm, which dimensionally corresponds to the sizes observed by Fresnel contrast imaging in the tablets. These results will serve for understanding the functions of intracrystalline organic macromolecules in mollusk shells.

  20. Measuring the shapes of macromolecules – and why it matters

    Directory of Open Access Journals (Sweden)

    Jie Li


    Full Text Available The molecular basis of life rests on the activity of biological macromolecules, mostly nucleic acids and proteins. A perhaps surprising finding that crystallized over the last handful of decades is that geometric reasoning plays a major role in our attempt to understand these activities. In this paper, we address this connection between geometry and biology, focusing on methods for measuring and characterizing the shapes of macromolecules. We briefly review existing numerical and analytical approaches that solve these problems. We cover in more details our own work in this field, focusing on the alpha shape theory as it provides a unifying mathematical framework that enable the analytical calculations of the surface area and volume of a macromolecule represented as a union of balls, the detection of pockets and cavities in the molecule, and the quantification of contacts between the atomic balls. We have shown that each of these quantities can be related to physical properties of the molecule under study and ultimately provides insight on its activity. We conclude with a brief description of new challenges for the alpha shape theory in modern structural biology.

  1. Measuring the shapes of macromolecules - and why it matters. (United States)

    Li, Jie; Mach, Paul; Koehl, Patrice


    The molecular basis of life rests on the activity of biological macromolecules, mostly nucleic acids and proteins. A perhaps surprising finding that crystallized over the last handful of decades is that geometric reasoning plays a major role in our attempt to understand these activities. In this paper, we address this connection between geometry and biology, focusing on methods for measuring and characterizing the shapes of macromolecules. We briefly review existing numerical and analytical approaches that solve these problems. We cover in more details our own work in this field, focusing on the alpha shape theory as it provides a unifying mathematical framework that enable the analytical calculations of the surface area and volume of a macromolecule represented as a union of balls, the detection of pockets and cavities in the molecule, and the quantification of contacts between the atomic balls. We have shown that each of these quantities can be related to physical properties of the molecule under study and ultimately provides insight on its activity. We conclude with a brief description of new challenges for the alpha shape theory in modern structural biology.

  2. Self-organization of amphiphilic macromolecules with local helix structure in concentrated solutions. (United States)

    Glagolev, M K; Vasilevskaya, V V; Khokhlov, A R


    Concentrated solutions of amphiphilic macromolecules with local helical structure were studied by means of molecular dynamic simulations. It is shown that in poor solvent the macromolecules are assembled into wire-like aggregates having complex core-shell structure. The core consists of a hydrophobic backbone of the chains which intertwine around each other. It is protected by the shell of hydrophilic side groups. In racemic mixture of right-hand and left-hand helix macromolecules the wire-like complex is a chain of braid bundles of macromolecules with the same chirality stacking at their ends. The average number of macromolecules in the wire cross-section is close to that of separate bundles observed in dilute solutions of such macromolecules. The effects described here could serve as a simple model of self-organization in solutions of macromolecules with local helical structure.

  3. Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering. (United States)

    Tria, Giancarlo; Mertens, Haydyn D T; Kachala, Michael; Svergun, Dmitri I


    Dynamic ensembles of macromolecules mediate essential processes in biology. Understanding the mechanisms driving the function and molecular interactions of 'unstructured' and flexible molecules requires alternative approaches to those traditionally employed in structural biology. Small-angle X-ray scattering (SAXS) is an established method for structural characterization of biological macromolecules in solution, and is directly applicable to the study of flexible systems such as intrinsically disordered proteins and multi-domain proteins with unstructured regions. The Ensemble Optimization Method (EOM) [Bernadó et al. (2007 ▶). J. Am. Chem. Soc. 129, 5656-5664] was the first approach introducing the concept of ensemble fitting of the SAXS data from flexible systems. In this approach, a large pool of macromolecules covering the available conformational space is generated and a sub-ensemble of conformers coexisting in solution is selected guided by the fit to the experimental SAXS data. This paper presents a series of new developments and advancements to the method, including significantly enhanced functionality and also quantitative metrics for the characterization of the results. Building on the original concept of ensemble optimization, the algorithms for pool generation have been redesigned to allow for the construction of partially or completely symmetric oligomeric models, and the selection procedure was improved to refine the size of the ensemble. Quantitative measures of the flexibility of the system studied, based on the characteristic integral parameters of the selected ensemble, are introduced. These improvements are implemented in the new EOM version 2.0, and the capabilities as well as inherent limitations of the ensemble approach in SAXS, and of EOM 2.0 in particular, are discussed.

  4. Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering

    Directory of Open Access Journals (Sweden)

    Giancarlo Tria


    Full Text Available Dynamic ensembles of macromolecules mediate essential processes in biology. Understanding the mechanisms driving the function and molecular interactions of `unstructured' and flexible molecules requires alternative approaches to those traditionally employed in structural biology. Small-angle X-ray scattering (SAXS is an established method for structural characterization of biological macromolecules in solution, and is directly applicable to the study of flexible systems such as intrinsically disordered proteins and multi-domain proteins with unstructured regions. The Ensemble Optimization Method (EOM [Bernadó et al. (2007. J. Am. Chem. Soc. 129, 5656–5664] was the first approach introducing the concept of ensemble fitting of the SAXS data from flexible systems. In this approach, a large pool of macromolecules covering the available conformational space is generated and a sub-ensemble of conformers coexisting in solution is selected guided by the fit to the experimental SAXS data. This paper presents a series of new developments and advancements to the method, including significantly enhanced functionality and also quantitative metrics for the characterization of the results. Building on the original concept of ensemble optimization, the algorithms for pool generation have been redesigned to allow for the construction of partially or completely symmetric oligomeric models, and the selection procedure was improved to refine the size of the ensemble. Quantitative measures of the flexibility of the system studied, based on the characteristic integral parameters of the selected ensemble, are introduced. These improvements are implemented in the new EOM version 2.0, and the capabilities as well as inherent limitations of the ensemble approach in SAXS, and of EOM 2.0 in particular, are discussed.

  5. Impacts of Organic Macromolecules, Chlorophyll and Soot on Arctic Sea Ice (United States)

    Ogunro, O. O.; Wingenter, O. W.; Elliott, S.; Flanner, M.; Dubey, M. K.


    Recent intensification of Arctic amplification can be strongly connected to positive feedback relating black carbon deposition to sea ice surface albedo. In addition to soot deposition on the ice and snow pack, ice algal chlorophyll is likely to compete as an absorber and redistributor of energy. Hence, solar radiation absorption by chlorophyll and some components of organic macromolecules in/under the ice column is currently being examined to determine the level of influence on predicted rate of ice loss. High amounts of organic macromolecules and chlorophyll are produced in global sea ice by the bottom microbial community and also in vertically distributed layers where substantial biological activities take place. Brine channeling in columnar ice can allow for upward flow of nutrients which leads to greater primary production in the presence of moderate light. Modeling of the sea-ice processes in tandem with experiments and field observations promises rapid progress in enhancing Arctic ice predictions. We are designing and conducting global climate model experiments to determine the impact of organic macromolecules and chlorophyll on Arctic sea ice. Influences on brine network permeability and radiation/albedo will be considered in this exercise. Absorption by anthropogenic materials such as soot and black carbon will be compared with that of natural pigments. We will indicate areas of soot and biological absorption dominance in the sense of single scattering, then couple into a full radiation transfer scheme to attribute the various contributions to polar climate change amplification. The work prepares us to study more traditional issues such as chlorophyll warming of the pack periphery and chemical effects of the flow of organics from ice internal communities. The experiments started in the Arctic will broaden to include Antarctic sea ice and shelves. Results from the Arctic simulations will be presented.

  6. 生物大分子结晶技术研究进展%Progress in Research of Technologies for Crystallization of Biological Macromolecules

    Institute of Scientific and Technical Information of China (English)

    徐乐慧; 洪鸣凰; 赵文杰; 任国宾


    获得具有高分辨率的晶体是目前生物大分子结构测定的主要瓶颈,生物大分子的结晶是一个复杂的物理化学过程,涉及多方面的影响因素.本文综述了近年来生物大分子结晶的热点方法和技术.%Obtaining the high-resolution crystal structure has become a bottleneck for structure determination of biological macromolecules. The crystallization of biological macromolecules is a complicated process influenced by many parameters. The recent technologies for preparing crystal of biological macromolecules as well as techniques for controlling the crystallization process are reviewed.

  7. Using pulse transit delay in Z-scan to discriminate between excited-state absorption and other nonlinear processes in ZnO nanocones. (United States)

    Shortell, Matthew P; Jaatinen, Esa A; Chang, Jin; Waclawik, Eric R


    We report a new approach that uses the single beam Z-scan technique, to discriminate between excited state absorption (ESA) and two and three photon nonlinear absorption. By measuring the apparent delay or advance of the pulse in reaching the detector, the nonlinear absorption can be unambiguously identified as either instantaneous or transient. The simple method does not require a large range of input fluences or sophisticated pulse-probe experimental apparatus. The technique is easily extended to any absorption process dependent on pulse width and to nonlinear refraction measurements. We demonstrate in particular, that the large nonlinear absorption in ZnO nanocones when exposed to nanosecond 532 nm pulses, is due mostly to ESA, not pure two-photon absorption.

  8. Determination of Constitutive Equation for Thermo-mechanical Processing of INCONEL 718 Through Double Multivariate Nonlinear Regression Analysis (United States)

    Hussain, Mirza Zahid; Li, Fuguo; Wang, Jing; Yuan, Zhanwei; Li, Pan; Wu, Tao


    The present study comprises the determination of constitutive relationship for thermo-mechanical processing of INCONEL 718 through double multivariate nonlinear regression, a newly developed approach which not only considers the effect of strain, strain rate, and temperature on flow stress but also explains the interaction effect of these thermo-mechanical parameters on flow behavior of the alloy. Hot isothermal compression experiments were performed on Gleeble-3500 thermo-mechanical testing machine in the temperature range of 1153 to 1333 K within the strain rate range of 0.001 to 10 s-1. The deformation behavior of INCONEL 718 is analyzed and summarized by establishing the high temperature deformation constitutive equation. The calculated correlation coefficient ( R) and average absolute relative error ( AARE) underline the precision of proposed constitutive model.

  9. Modeling of long-range memory processes with inverse cubic distributions by the nonlinear stochastic differential equations (United States)

    Kaulakys, B.; Alaburda, M.; Ruseckas, J.


    A well-known fact in the financial markets is the so-called ‘inverse cubic law’ of the cumulative distributions of the long-range memory fluctuations of market indicators such as a number of events of trades, trading volume and the logarithmic price change. We propose the nonlinear stochastic differential equation (SDE) giving both the power-law behavior of the power spectral density and the long-range dependent inverse cubic law of the cumulative distribution. This is achieved using the suggestion that when the market evolves from calm to violent behavior there is a decrease of the delay time of multiplicative feedback of the system in comparison to the driving noise correlation time. This results in a transition from the Itô to the Stratonovich sense of the SDE and yields a long-range memory process.

  10. Non-linear optical processes involving excited subbands in laser-dressed quantum wires with triangular cross-section (United States)

    Radu, A.; Duque, C. A.


    The conduction subband structure of a triangular cross-section GaAs/AlGaAs quantum well wire under intense laser field is theoretically investigated by taking into account a finite confining potential. The calculation of the subband energy levels is based on a two-dimensional finite element method within the effective mass approximation. It is shown that a transversally polarized laser field non-uniformly shifts the subband energy levels and could be used for tuning the intersubband transitions and altering the related optical susceptibilities. We found that the non-resonant laser field allows the magnification and the red- or blueshift of the third-order non-linear susceptibility peaks for particular polarizations of the pump light and proper laser parameter values. The effects of the laser dressing field on the intersubband third harmonic generation and quadratic electro-optical process are discussed.

  11. Experimental validation of an optimized signal processing method to handle non-linearity in swept-source optical coherence tomography. (United States)

    Vergnole, Sébastien; Lévesque, Daniel; Lamouche, Guy


    We evaluate various signal processing methods to handle the non-linearity in wavenumber space exhibited by most laser sources for swept-source optical coherence tomography. The following methods are compared for the same set of experimental data: non-uniform discrete Fourier transforms with Vandermonde matrix or with Lomb periodogram, resampling with linear interpolation or spline interpolation prior to fast-Fourier transform (FFT), and resampling with convolution prior to FFT. By selecting an optimized Kaiser-Bessel window to perform the convolution, we show that convolution followed by FFT is the most efficient method. It allows small fractional oversampling factor between 1 and 2, thus a minimal computational time, while retaining an excellent image quality. (c) 2010 Optical Society of America.

  12. Brain signal variability as a window into the bidirectionality between music and language processing: moving from a linear to a nonlinear model. (United States)

    Hutka, Stefanie; Bidelman, Gavin M; Moreno, Sylvain


    There is convincing empirical evidence for bidirectional transfer between music and language, such that experience in either domain can improve mental processes required by the other. This music-language relationship has been studied using linear models (e.g., comparing mean neural activity) that conceptualize brain activity as a static entity. The linear approach limits how we can understand the brain's processing of music and language because the brain is a nonlinear system. Furthermore, there is evidence that the networks supporting music and language processing interact in a nonlinear manner. We therefore posit that the neural processing and transfer between the domains of language and music are best viewed through the lens of a nonlinear framework. Nonlinear analysis of neurophysiological activity may yield new insight into the commonalities, differences, and bidirectionality between these two cognitive domains not measurable in the local output of a cortical patch. We thus propose a novel application of brain signal variability (BSV) analysis, based on mutual information and signal entropy, to better understand the bidirectionality of music-to-language transfer in the context of a nonlinear framework. This approach will extend current methods by offering a nuanced, network-level understanding of the brain complexity involved in music-language transfer.

  13. On the processing of highly nonlinear solitary waves and guided ultrasonic waves for structural health monitoring and nondestructive evaluation (United States)

    Bagheri, Abdollah

    The in-situ measurement of thermal stress in civil and mechanical structures may prevent structural anomalies such as unexpected buckling. In the first half of the dissertation, we present a study where highly nonlinear solitary waves (HNSWs) were utilized to measure axial stress in slender beams. HNSWs are compact non-dispersive waves that can form and travel in nonlinear systems such as one-dimensional chains of particles. The effect of the axial stress acting in a beam on the propagation of HNSWs was studied. We found that certain features of the solitary waves enable the measurement of the stress. In general, most guided ultrasonic waves (GUWs)-based health monitoring approaches for structural waveguides are based on the comparison of testing data to baseline data. In the second half of the dissertation, we present a study where some baseline-free signal processing algorithms were presented and applied to numerical and experimental data for the structural health monitoring (SHM) of underwater or dry structures. The algorithms are based on one or more of the following: continuous wavelet transform, empirical mode decomposition, Hilbert transform, competitive optimization algorithm, probabilistic methods. Moreover, experimental data were also processed to extract some features from the time, frequency, and joint time-frequency domains. These features were then fed to a supervised learning algorithm based on artificial neural networks to classify the types of defect. The methods were validated using the numerical model of a plate and a pipe, and the experimental study of a plate in water. In experiment, the propagation of ultrasonic waves was induced by means of laser pulses or transducer and detected with an array of immersion transducers. The results demonstrated that the algorithms are effective, robust against noise, and able to localize and classify the damage.

  14. Analysis of acceptable spectral windows of quadratic cascaded nonlinear processes in a periodically poled lithium niobate waveguide. (United States)

    Lee, Kwang Jo; Liu, Sheng; Gallo, Katia; Petropoulos, Periklis; Richardson, David J


    We report a systematic and comparative study of the acceptance bandwidths of two cascaded quadratic nonlinear processes in periodically poled lithium niobate waveguides, namely cascaded second-harmonic generation and difference-frequency generation (cSHG/DFG) and cascaded sum-frequency generation and difference-frequency generation (cSFG/DFG). We first theoretically and experimentally study the acceptance bandwidths of both the individual second-harmonic generation (SHG) and sum-frequency generation (SFG) processes in the continuous wave (CW) and pulsed-pump regimes. Our results show that the SHG bandwidth is approximately half that of the SFG process in the CW regime, whereas the SHG acceptance bandwidth can approach the CW SFG bandwidth limit when pulsed-pump is used. As a consequence we conclude that the tuning bandwidths of both cascaded processes should be similar in the pulsed pump regime once the pump pulse bandwidths approach that of SFG (i.e. the cSHG/DFG bandwidth is not limited by the CW SHG bandwidth). We confirm that this is the case experimentally.

  15. Supramolecular Assembly of Comb-like Macromolecules Induced by Chemical Reactions that Modulate the Macromolecular Interactions In Situ. (United States)

    Xia, Hongwei; Fu, Hailin; Zhang, Yanfeng; Shih, Kuo-Chih; Ren, Yuan; Anuganti, Murali; Nieh, Mu-Ping; Cheng, Jianjun; Lin, Yao


    Supramolecular polymerization or assembly of proteins or large macromolecular units by a homogeneous nucleation mechanism can be quite slow and require specific solution conditions. In nature, protein assembly is often regulated by molecules that modulate the electrostatic interactions of the protein subunits for various association strengths. The key to this regulation is the coupling of the assembly process with a reversible or irreversible chemical reaction that occurs within the constituent subunits. However, realizing this complex process by the rational design of synthetic molecules or macromolecules remains a challenge. Herein, we use a synthetic polypeptide-grafted comb macromolecule to demonstrate how the in situ modulation of interactions between the charged macromolecules affects their resulting supramolecular structures. The kinetics of structural formation was studied and can be described by a generalized model of nucleated polymerization containing secondary pathways. Basic thermodynamic analysis indicated the delicate role of the electrostatic interactions between the charged subunits in the reaction-induced assembly process. This approach may be applicable for assembling a variety of ionic soft matters that are amenable to chemical reactions in situ.

  16. Grid Size Selection for Nonlinear Least-Squares Optimization in Spectral Estimation and Array Processing

    DEFF Research Database (Denmark)

    Nielsen, Jesper Kjær; Jensen, Tobias Lindstrøm; Jensen, Jesper Rindom;


    time. Additionally, we show via three common examples how the grid size depends on parameters such as the number of data points or the number of sensors in DOA estimation. We also demonstrate that the computation time can potentially be lowered by several orders of magnitude by combining a coarse grid......In many spectral estimation and array processing problems, the process of finding estimates of model parameters often involves the optimisation of a cost function containing multiple peaks and dips. Such non-convex problems are hard to solve using traditional optimisation algorithms developed...

  17. Nonlinear Parasitic Capacitance Modelling of High Voltage Power MOSFETs in Partial SOI Process

    DEFF Research Database (Denmark)

    Fan, Lin; Knott, Arnold; Jørgensen, Ivan Harald Holger


    : off-state, sub-threshold region, and on-state in the linear region. A high voltage power MOSFET is designed in a partial Silicon on Insulator (SOI) process, with the bulk as a separate terminal. 3D plots and contour plots of the capacitances versus bias voltages for the transistor summarize...

  18. Optimization of Nonlinear Figure-of-Merits of Integrated Power MOSFETs in Partial SOI Process

    DEFF Research Database (Denmark)

    Fan, Lin; Jørgensen, Ivan Harald Holger; Knott, Arnold


    different operating conditions. A systematic analysis of the optimization of these FOMs has not been previously established. The optimization methods are verified on a 100 V power MOSFET implemented in a 0.18 µm partial SOI process. Its FOMs are lowered by 1.3-18.3 times and improved by 22...

  19. Modeling human auditory evoked brainstem responses based on nonlinear cochlear processing

    DEFF Research Database (Denmark)

    Harte, James; Rønne, Filip Munch; Dau, Torsten


    (ABR) to transient sounds and frequency following responses (FFR) to tones. The model includes important cochlear processing stages (Zilany and Bruce, 2006) such as basilar-membrane (BM) tuning and compression, inner hair-cell (IHC) transduction, and IHC auditory-nerve (AN) synapse adaptation...

  20. Investigation of the nonlinear effects during the sedimentation process of a charged colloidal particle by direct numerical simulation. (United States)

    Keller, Florian; Feist, Markus; Nirschl, Hermann; Dörfler, Willy


    In this article we study the settling process of a colloidal particle under the influence of a gravitational or centrifugal field in an unbounded electrolyte solution. Since particles in aqueous solutions normally carry a non-zero surface charge, a microscopic electric field develops which alters the sedimentation process compared to an uncharged particle. This process can be mathematically modelled via the Stokes-Poisson-Nernst-Planck system, a system of coupled partial differential equations that have to be solved in an exterior domain. After a dimensional analysis we investigate the influence of the various characteristic dimensionless numbers on the sedimentation velocity. Thereby the linear-response (weak-field) approximation that underpins almost all existing theoretical work on classical electrokinetic phenomena is relaxed, such that no additional assumption on the thickness of the double layer as well as on its displacement is needed. We show that there exists a strong influence of the fluid Reynolds number and the ionic strength on the sedimentation velocity. Further we have developed an asymptotic expansion to describe the limit of small values of the surface potential of a single particle. This expansion incorporates all nonlinear effects and extends the well-known results of Booth (1954) [1] and Ohshima et al. (1984) [2] to higher fluid Reynolds numbers.

  1. Modified kernel principal component analysis using double-weighted local outlier factor and its application to nonlinear process monitoring. (United States)

    Deng, Xiaogang; Wang, Lei


    Traditional kernel principal component analysis (KPCA) based nonlinear process monitoring method may not perform well because its Gaussian distribution assumption is often violated in the real industrial processes. To overcome this deficiency, this paper proposes a modified KPCA method based on double-weighted local outlier factor (DWLOF-KPCA). In order to avoid the assumption of specific data distribution, local outlier factor (LOF) is introduced to construct two LOF-based monitoring statistics, which are used to substitute for the traditional T(2) and SPE statistics, respectively. To provide better online monitoring performance, a double-weighted LOF method is further designed, which assigns the weights for each component to highlight the key components with significant fault information, and uses the moving window to weight the historical statistics for reducing the drastic fluctuations in the monitoring results. Finally, simulations on a numerical example and the Tennessee Eastman (TE) benchmark process are used to demonstrate the superiority of the proposed DWLOF-KPCA method. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  2. Equilibrium theory-based analysis of nonlinear waves in separation processes. (United States)

    Mazzotti, Marco; Rajendran, Arvind


    Different areas of engineering, particularly separation process technology, deal with one-dimensional, nonstationary processes that under reasonable assumptions, namely negligible dispersion effects and transport resistances, are described by mathematical models consisting of systems of first-order partial differential equations. Their behavior is characterized by continuous or discontinuous composition (or thermal) fronts that propagate along the separation unit. The equilibrium theory (i.e., the approach discussed here to determine the solution to these model equations) predicts this with remarkable accuracy, despite the simplifications and assumptions. Interesting applications are in adsorption, chromatography and ion-exchange, distillation, gas injection, heat storage, sedimentation, precipitation, and dissolution waves. We show how mathematics can enlighten the engineering aspects, and we guide the researcher not only to reach a synthetic understanding of properties of fundamental and applicative interest but also to discover new, unexpected, and fascinating phenomena. The tools presented here are useful to teachers, researchers, and practitioners alike.

  3. Linear and nonlinear post-processing of numerically forecasted surface temperature

    Directory of Open Access Journals (Sweden)

    M. Casaioli


    Full Text Available In this paper we test different approaches to the statistical post-processing of gridded numerical surface air temperatures (provided by the European Centre for Medium-Range Weather Forecasts onto the temperature measured at surface weather stations located in the Italian region of Puglia. We consider simple post-processing techniques, like correction for altitude, linear regression from different input parameters and Kalman filtering, as well as a neural network training procedure, stabilised (i.e. driven into the absolute minimum of the error function over the learning set by means of a Simulated Annealing method. A comparative analysis of the results shows that the performance with neural networks is the best. It is encouraging for systematic use in meteorological forecast-analysis service operations.

  4. Cryo-electron microscopy for structural analysis of dynamic biological macromolecules. (United States)

    Murata, Kazuyoshi; Wolf, Matthias


    Since the introduction of what became today's standard for cryo-embedding of biological macromolecules at native conditions more than 30years ago, techniques and equipment have been drastically improved and the structure of biomolecules can now be studied at near atomic resolution by cryo-electron microscopy (cryo-EM) while capturing multiple dynamic states. Here we review the recent progress in cryo-EM for structural studies of dynamic biological macromolecules. We provide an overview of the cryo-EM method and introduce contemporary studies to investigate biomolecular structure and dynamics, including examples from the recent literature. Cryo-EM is a powerful tool for the investigation of biological macromolecular structures including analysis of their dynamics by using advanced image-processing algorithms. The method has become even more widely applicable with present-day single particle analysis and electron tomography. The cryo-EM method can be used to determine the three-dimensional structure of biomacromolecules in near native condition at close to atomic resolution, and has the potential to reveal conformations of dynamic molecular complexes. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  5. Coupled Segmentation of Nuclear and Membrane-bound Macromolecules through Voting and Multiphase Level Set. (United States)

    Chang, Hang; Wen, Quan; Parvin, Bahram


    Membrane-bound macromolecules play an important role in tissue architecture and cell-cell communication, and is regulated by almost one-third of the genome. At the optical scale, one group of membrane proteins expresses themselves as linear structures along the cell surface boundaries, while others are sequestered; and this paper targets the former group. Segmentation of these membrane proteins on a cell-by-cell basis enables the quantitative assessment of localization for comparative analysis. However, such membrane proteins typically lack continuity, and their intensity distributions are often very heterogeneous; moreover, nuclei can form large clump, which further impedes the quantification of membrane signals on a cell-by-cell basis. To tackle these problems, we introduce a three-step process to (i) regularize the membrane signal through iterative tangential voting, (ii) constrain the location of surface proteins by nuclear features, where clumps of nuclei are segmented through a delaunay triangulation approach, and (iii) assign membrane-bound macromolecules to individual cells through an application of multi-phase geodesic level-set. We have validated our method using both synthetic data and a dataset of 200 images, and are able to demonstrate the efficacy of our approach with superior performance.

  6. Complexation between a macromolecule and an amphiphile by Monte Carlo technique. (United States)

    Gharibi, Hussein; Behjatmanesh-Ardakani, Reza; Hashemianzadeh, Majid; Mousavi-Khoshdel, Morteza


    Using a simple modified version of Larson's model, we studied the complexation between a macromolecule and an amphiphile in a dilute range of concentrations. The main characteristic of amphiphile molecules, that is, the hydrophobicity of the tails and hydrophilicity of the heads, is used to model the self-assembling process. Contrary to the molecular thermodynamics approaches, no prior shape was considered for the aggregates and the system was allowed to choose the most stable structure. For true ensemble averaging, without any synthetic results, configurational bias Monte Carlo and reptation moves are used to produce a Markov chain of configurations. From the results, it is found that the macromolecule causes the clusters of surfactants to be formed at a concentration much lower than the critical micelle concentration. Furthermore, the shape of the clusters tends to be more spherical, which is in line with theory and experiments. From the results, it is learned how a polymer can change the behavior of an amphiphilic molecule. All of the results are in good qualitative agreement with experimental and molecular thermodynamics results. Furthermore, the model predicts network formation between bound clusters at high concentrations of the surfactant.

  7. New Robust Nonlinear Controller Design Based on Predictive Control for Industrial Processes

    Directory of Open Access Journals (Sweden)

    Hossein Esfroghy


    Full Text Available In this paper a new sliding mode controller based on predictive control is used for the first order system, which is a good model for the industrial process. In this method a developed predictive control is used to optimize the sliding mode control including sliding surface and switching function coefficient at every moment. A new smooth function is used to reduce the chattering problems. Simulation results show the high effectiveness of the proposed controller.

  8. Nonlinear mathematical models for simulation modeling of dynamic processes in feed systems of cutter loaders

    Energy Technology Data Exchange (ETDEWEB)

    Gorbatov, P.A.; Plyungin, A.V. (Donetskii Politekhnicheskii Institut (USSR))


    Presents calculation methods and mathematical models of dynamic processes that occur in feed systems of cutter loaders with rigid pulling elements. Characteristics of dynamic interactions between driving wheels and the working section of the pulling system are taken into account. Mathematical models are given that describe the dynamic operation of the feed system. A method for calculation of a hydraulic vibration compensating system and its mathematical model is presented. Effectiveness of the vibration compensating system is discussed. 2 refs.

  9. A straightforward approximate analysis of Kerr nonlinear processes in sub-wavelength diameter optical fiber with better accuracy over variational technique (United States)

    Sadhu, Arunangshu; Sarkar, Somenath


    We report a simple and straightforward approximate analysis to investigate the effect of Kerr type nonlinear optical processes in sub-wavelength diameter step index optical fibers based on Marcuse method in single mode region. Optimum core diameters of such fibers, predicted by us, together with relevant core nonlinearity coefficient and effective area are seen to be compatible with the analytical values indicating the validity of this novel application of the elegant approximate method. However, the corresponding values, obtained by earlier variational method, show larger discrepancy with analytical findings in comparison with ours. Also, maximum enhancement of nonlinear processes within single mode region, confirming almost the analytical method, assures less diffraction. Formulations, coupled with simplicity and novelty of the present analysis, should find wide use by system users and experimentalists in this emerging area.

  10. Bending dynamics of semi-flexible macromolecules in isotropic turbulence

    CERN Document Server

    Ali, Aamir; Vincenzi, Dario


    We study the Lagrangian dynamics of semi-flexible macromolecules in laminar as well as in homogeneous and isotropic turbulent flows by means of analytically solvable stochastic models and direct numerical simulations. The statistics of the bending angle is qualitatively different in laminar and turbulent flows and exhibits a strong dependence on the topology of the velocity field. In particular, in two-dimensional turbulence, particles are either found in a fully extended or in a fully folded configuration; in three dimensions, the predominant configuration is the fully extended one.

  11. Conformations of Macromolecules and their Complexes from Heterogeneous Datasets

    CERN Document Server

    Schwander, P; Ourmazd, A


    We describe a new generation of algorithms capable of mapping the structure and conformations of macromolecules and their complexes from large ensembles of heterogeneous snapshots, and demonstrate the feasibility of determining both discrete and continuous macromolecular conformational spectra. These algorithms naturally incorporate conformational heterogeneity without resort to sorting and classification, or prior knowledge of the type of heterogeneity present. They are applicable to single-particle diffraction and image datasets produced by X-ray lasers and cryo-electron microscopy, respectively, and particularly suitable for systems not easily amenable to purification or crystallization.

  12. Analog CMOS Nonlinear Cells and Their Applications in VLSI Signal and Information Processing (United States)

    Khachab, Nabil Ibrahim


    The development of reconfigurable analog CMOS building blocks and their applications in analog VLSI is discussed and introduced in much the same way a logic gate is used in digital VLSI. They simultaneously achieve four -quadrant multiplication and division. These applications include multiplication, signal squaring, division, signal inversion, amplitude modulation. New all MOS implementations of the Hopfield like neural networks are developed by using the new cells. In addition new and novel techniques for sensor linearization and for MOSFET-C programmable-Q and omega_{n} filters are introduced. The new designs are simple, versatile, programmable and make effective use of analog CAD tools. Moreover, they are easily extendable to other technologies such as GaAs and BiCMOS. The objective of these designs is to achieve reduction in Silicon area and power consumption and reduce the interconnections between cells. It is also sought to provide a robust design that is CAD-compatible and make effective use of the standard cell library approach. This will offer more versatility and flexibility for analog signal processing systems and neural networks. Some of these new cells and a 3-neuron neural system are fabricated in a 2mum CMOS process. Experimental results of these circuits verify the validity of this new design approach.

  13. 3D modelling of non-linear visco-elasto-plastic crustal and lithospheric processes using LaMEM (United States)

    Popov, Anton; Kaus, Boris


    LaMEM (Lithosphere and Mantle Evolution Model) is a three-dimensional thermo-mechanical numerical code to simulate crustal and lithospheric deformation. The code is based on a staggered finite difference (FDSTAG) discretization in space, which is a stable and very efficient technique to solve the (nearly) incompressible Stokes equations that does not suffer from spurious pressure modes or artificial compressibility (a typical feature of low-order finite element techniques). Higher order finite element methods are more accurate than FDSTAG methods under idealized test cases where the jump in viscosity is exactly aligned with the boundaries of the elements. Yet, geodynamically more realistic cases involve evolving subduction zones, nonlinear rheologies or localized plastic shear bands. In these cases, the viscosity pattern evolves spontaneously during a simulation or even during nonlinear iterations, and the advantages of higher order methods disappear and they all converge with approximately first order accuracy, similar to that of FDSTAG [1]. Yet, since FDSTAG methods have considerably less degrees of freedom than quadratic finite element methods, they require about an order of magnitude less memory for the same number of nodes in 3D which also implies that every matrix-vector multiplication is significantly faster. LaMEM is build on top of the PETSc library and uses the particle-in-cell technique to track material properties, history variables which makes it straightforward to incorporate effects like phase changes or chemistry. An internal free surface is present, together with (simple) erosion and sedimentation processes, and a number of methods are available to import complex geometries into the code (e.g, Customized Galerkin coupled geometric multigrid preconditioners are implemented which resulted in a good parallel scalability of the code (we have tested LaMEM on 458'752 cores [2]). Yet, the drawback of using FDSTAG


    Institute of Scientific and Technical Information of China (English)

    Hong-xia Xi; Zhong Li; Zhao-xi Liang


    A new organic/inorganic hybrid nonlinear optical (NLO) material was developed by the sol-gel process of an alkoxysilane dye with tetraethoxysilane. A NLO moiety based on 4-nitro-4′-hydroxy azobenzene was covalently bonded to the triethoxysilane derivative, i.e. γ-isocyanatopropyl triethoxysilane. The preparation process and properties of the sol-gel derived NLO polymer were studied and characterized by SEM, FTIR, 1H-NMR, UV-Vis, DSC and second harmonic generation (SHG) measurement. The results indicated that the chemical bonding of the chromophores to the inorganic SiO2 networks induces Iow dipole alignment relaxation and preferable orientational stability. The SHG measurements also showed that the bonded polymer film containing 75 wt% of the akoxysilane dye has a high electro-optic coefficient (r33) of7. 1 pm/V at 1.1 μm wavelength, and exhibit good SHG stability, the r33 values can maintain about 92.7% of its initial value at room temperature for 90 days, and can maintain about 59.3% at 100℃ for 300 min.``

  15. High-order nonlinear optical processes in ablated carbon-containing materials: Recent approaches in development of the nonlinear spectroscopy using harmonic generation in the extreme ultraviolet range (United States)

    Ganeev, R. A.


    The nonlinear spectroscopy using harmonic generation in the extreme ultraviolet range became a versatile tool for the analysis of the optical, structural and morphological properties of matter. The carbon-contained materials have shown the advanced properties among other studied species, which allowed both the definition of the role of structural properties on the nonlinear optical response and the analysis of the fundamental features of carbon as the attractive material for generation of coherent short-wavelength radiation. We review the studies of the high-order harmonic generation by focusing ultrashort pulses into the plasmas produced during laser ablation of various organic compounds. We discuss the role of ionic transitions of ablated carbon-containing molecules on the harmonic yield. We also show the similarities and distinctions of the harmonic and plasma spectra of organic compounds and graphite. We discuss the studies of the generation of harmonics up to the 27th order (λ = 29.9 nm) of 806 nm radiation in the boron carbide plasma and analyze the advantages and disadvantages of this target compared with the ingredients comprising B4C (solid boron and graphite) by comparing plasma emission and harmonic spectra from three species. We also show that the coincidence of harmonic and plasma emission wavelengths in most cases does not cause the enhancement or decrease of the conversion efficiency of this harmonic.

  16. Multi input single output model predictive control of non-linear bio-polymerization process

    Energy Technology Data Exchange (ETDEWEB)

    Arumugasamy, Senthil Kumar; Ahmad, Z. [School of Chemical Engineering, Univerisiti Sains Malaysia, Engineering Campus, Seri Ampangan,14300 Nibong Tebal, Seberang Perai Selatan, Pulau Pinang (Malaysia)


    This paper focuses on Multi Input Single Output (MISO) Model Predictive Control of bio-polymerization process in which mechanistic model is developed and linked with the feedforward neural network model to obtain a hybrid model (Mechanistic-FANN) of lipase-catalyzed ring-opening polymerization of ε-caprolactone (ε-CL) for Poly (ε-caprolactone) production. In this research, state space model was used, in which the input to the model were the reactor temperatures and reactor impeller speeds and the output were the molecular weight of polymer (M{sub n}) and polymer polydispersity index. State space model for MISO created using System identification tool box of Matlab™. This state space model is used in MISO MPC. Model predictive control (MPC) has been applied to predict the molecular weight of the biopolymer and consequently control the molecular weight of biopolymer. The result shows that MPC is able to track reference trajectory and give optimum movement of manipulated variable.

  17. All-Optical Signal processing using Highly Nonlinear Photonic Crystal Fiber

    DEFF Research Database (Denmark)

    Andersen, Peter Andreas


    The use of HNL-PCF in optical communication systems has been investigated in this thesis. The investigation has been done with respect to the future of telecommunications in an all-optical system. The PCFs used have all been used for all-optical signal processing as part of an optical component...... and the possibility of large differences between the refractive indices of the core and the cladding by using air-holes, makes PCFs suited for custom made components. By testing a HNL-PCF as a medium for supercontinuum generation at various dispersion values and at the same time using that supercontinuum...... of modulation format of the signal. The modulation format is also dependent on transmission in the optical system and dependent on the pulse source used to generate the supercontinuum. It is believed that by satisfying strict demands on the pulse sources and the fiber design, could the use of a supercontinuum...

  18. Discontinuous Galerkin methods on graphics processing units for nonlinear hyperbolic conservation laws

    CERN Document Server

    Fuhry, Martin; Krivodonova, Lilia


    We present a novel implementation of the modal discontinuous Galerkin (DG) method for hyperbolic conservation laws in two dimensions on graphics processing units (GPUs) using NVIDIA's Compute Unified Device Architecture (CUDA). Both flexible and highly accurate, DG methods accommodate parallel architectures well as their discontinuous nature produces element-local approximations. High performance scientific computing suits GPUs well, as these powerful, massively parallel, cost-effective devices have recently included support for double-precision floating point numbers. Computed examples for Euler equations over unstructured triangle meshes demonstrate the effectiveness of our implementation on an NVIDIA GTX 580 device. Profiling of our method reveals performance comparable to an existing nodal DG-GPU implementation for linear problems.

  19. Self-Organized Criticality in Astrophysics The Statistics of Nonlinear Processes in the Universe

    CERN Document Server

    Aschwanden, Markus


    The concept of ‘self-organized criticality’ (SOC) has been applied to a variety of problems, ranging from population growth and traffic jams to earthquakes, landslides and forest fires. The technique is now being applied to a wide range of phenomena in astrophysics, such as planetary magnetospheres, solar flares, cataclysmic variable stars, accretion disks, black holes and gamma-ray bursts, and also to phenomena in galactic physics and cosmology. Self-organized Criticality in Astrophysics introduces the concept of SOC and shows that, due to its universality and ubiquity, it is a law of nature. The theoretical framework and specific physical models are described, together with a range of applications in various aspects of astrophyics. The mathematical techniques, including the statistics of random processes, time series analysis, time scale and waiting time distributions, are presented and the results are applied to specific observations of astrophysical phenomena.

  20. Arrangement of a nanostructure array to control equilibrium and nonequilibrium transports of macromolecules. (United States)

    Yasui, Takao; Kaji, Noritada; Ogawa, Ryo; Hashioka, Shingi; Tokeshi, Manabu; Horiike, Yasuhiro; Baba, Yoshinobu


    Exploiting the nonequilibrium transport of macromolecules makes it possible to increase the separation speed without any loss of separation resolution. Here we report the arrangement of a nanostructure array in microchannels to control equilibrium and nonequilibrium transports of macromolecules. The direct observation and separation of macromolecules in the nanopillar array reported here are the first to reveal the nonequilibrium transport, which has a potential to overcome the intrinsic trade-off between the separation speed and resolution.

  1. Dual Use of Amphiphilic Macromolecules As Cholesterol Efflux Triggers and Inhibitors of Macrophage Athero-inflammation (United States)

    Iverson, Nicole; Plourde, Nicole M.; Sparks, Sarah M.; Wang, Jinzhong; Patel, Ekta; Shah, Pratik; Lewis, Daniel R.; Zablocki, Kyle; Nackman, Gary B.; Uhrich, Kathryn E.; Moghe, Prabhas V.


    Activated vascular wall macrophages can rapidly internalize modified lipoproteins and escalate the growth of atherosclerotic plaques. This article proposes a biomaterials-based therapeutic intervention for depletion of non-regulated cholesterol accumulation and inhibition of inflammation of macrophages. Macromolecules with high scavenger receptor (SR)-binding activity were investigated for SR-mediated delivery of agonists to cholesterol-trafficking nuclear liver-X receptors. From a diverse feature space of a family of amphiphilic macromolecules of linear and aromatic mucic acid backbones modified with varied aliphatic chains and conjugated with differentially branched poly(ethylene glycol), a key molecule (carboxyl-terminated, C12-derivatized, linear mucic acid backbone) was selected for its ability to preferentially bind scavenger receptor A (SR-A) as the key target. At a basal level, this macromolecule suppressed the pro-inflammatory signaling of activated THP-1 macrophages while competitively lowering oxLDL uptake in vitro through scavenger receptor SRA-1 targeting. To further deplete intracellular cholesterol, the core macromolecule structure was exploited to solubilize a hydrophobic small molecule agonist for nuclear Liver-X Receptors, which regulate the efflux of intracellular cholesterol. The macromolecule-encapsulated agonist system was found to reduce oxLDL accumulation by 88% in vitro in comparison to controls. In vivo studies were designed to release the macromolecules (with or without encapsulated agonist) to injured carotid arteries within Sprague Dawley rats fed a high fat diet, conditions that yield enhanced cholesterol accumulation and macrophage recruitment. The macromolecules lowered intimal levels of accumulated cholesterol (50% for macromolecule alone; 70% for macromolecule-encapsulated agonist) and inhibited macrophage retention (92% for macromolecule; 96% for macromolecule-encapsulated agonist; 4 days) relative to non-treated controls. Thus

  2. Ultrafast and nonlinear optical characterization of optical limiting processes in fullerenes

    Energy Technology Data Exchange (ETDEWEB)

    Kohlman, R.; Klimov, V.; Shi, X. [and others


    The authors present recent results of broadband femotosecond (fs) transient absorption (TA) and broadband nanosecond (ns) optical limiting (OL) studies of C{sub 60} and derivatized C{sub 60}. Improvements in measurement techniques for fs TA spectra provide sensitivity to 10{sup {minus}5} in differential transmission, allowing detailed comparison of excited-state spectra with established energy level diagrams, as well as comparison of the ratio of triplet to singlet excited-state absorption cross sections from TA spectra with those obtained by modeling time transients at different wavelengths. For derivatized fullerenes, which provide enhanced solubility and a ground-state absorption extended into the infrared compared with C{sub 60} there is no spectral region where the triplet absorption cross section dominates the singlet as strongly as demonstrating broadband limiting in all 6, 6 mono-adducts and neat C{sub 60}. The authors report new approaches to processing sol-gel encapsulated fullerenes to improve the OL performance of solid-state materials to approach the response of solution limiters.

  3. Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease

    DEFF Research Database (Denmark)

    Parlesak, Alexandr; Schäfer, C.; Schütz, Tanja


    BACKGROUND/AIMS: No information is yet available about the influence of alcohol abuse on the translocation of larger molecules (Mr>1200) through the intestinal mucosa in man. The present study aimed to determine the intestinal permeability to macromolecules in patients with chronic alcohol abuse ...... the translocation of bacterial toxins, thereby contributing to inflammatory processes in alcoholic liver disease....

  4. Selective extraction of proteins and other macromolecules from biological samples using molecular imprinted polymers. (United States)

    Stevenson, Derek; El-Sharif, Hazim F; Reddy, Subrayal M


    The accurate determination of intact macromolecules in biological samples, such as blood, plasma, serum, urine, tissue and feces is a challenging problem. The increased interest in macromolecules both as candidate drugs and as biomarkers for diagnostic purposes means that new method development approaches are needed. This review charts developments in the use of molecularly imprinted polymers first for small-molecular-mass compounds then for proteins and other macromolecules. Examples of the development of molecularly imprinted polymers for macromolecules are highlighted. The two main application areas to date are sensors and separation science, particularly SPE. Examples include peptides and polypeptides, lysozyme, hemoglobin, ovalbumin, bovine serum albumin and viruses.

  5. Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation. (United States)

    Magenau, Andrew J D; Saurabh, Saumya; Andreko, Susan K; Telmer, Cheryl A; Schmidt, Brigitte F; Waggoner, Alan S; Bruchez, Marcel P


    The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling of subcellular structures was realized at nanomolar concentrations of polymer, in the absence of membrane permeabilization or transduction domains, and fluorogen-modified polymers were found to bind to protein intact after delivery to the cytosol. Cellular motility was found to be dependent on binding of macromolecular fluorogens to actin structures causing rapid cellular ruffling without migration. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Growth of single crystals of organic salts with large second-order optical nonlinearities by solution processes for devices (United States)

    Leslie, Thomas M.


    Data obtained from the electric field induced second harmonic generation (EFISH) and Kurtz Powder Methods will be provided to MSFC for further refinement of their method. A theoretical model for predicting the second-order nonlinearities of organic salts is being worked on. Another task is the synthesis of a number of salts with various counterions. Several salts with promising SHG activities and new salts will be tested for the presence of two crystalline forms. The materials will be recrystallized from dry and wet solvents and compared for SHG efficiency. Salts that have a high SHG efficiency and no tendency to form hydrates will be documented. The synthesis of these materials are included in this report. A third task involves method to aid in the growth of large, high quality single crystals by solution processes. These crystals will be characterized for their applicability in the fabrication of devices that will be incorporated into optical computers in future programs. Single crystals of optimum quality may be obtained by crystal growth in low-gravity. The final task is the design of a temperature lowering single crystal growth apparatus for ground based work. At least one prototype will be built.

  7. Nonlinear Processes in Plasmas. (United States)


    27. P. N. Guzdar, L. Chen, P. K. Kaw and C. Oberman, "Effect of Magnetic Shear on the Drift Dissipative Instability," Physical Review Letters , 40...1566-1570, 1978. 28. L. Chen, P. N. Guzdar, R. B. White, P. K. Kaw and C. Oberman, "Theory of the Universal Drift Instability," Physical Review Letters 41...34Particle Diffusion by Magnetic Perturbations of Axisymmetric Geometries," Physical Review Letters 43, 1506-1509, 1979. 42. P. K. Kaw, E. 3. Valeo and P. H

  8. Recent Advances in Non-Invasive Delivery of Macromolecules using Nanoparticulate Carriers System. (United States)

    Shadab, Md; Haque, Shadabul; Sheshala, Ravi; Meng, Lim Wei; Meka, Venkata Srikanth; Ali, Javed


    The drug delivery of macromolecules such as proteins and peptides has become an important area of research and represents the fastest expanding share of the market for human medicines. The most common method for delivering macromolecules is parenterally. However parenteral administration of some therapeutic macromolecules has not been effective because of their rapid clearance from the body. As a result, most macromolecules are only therapeutically useful after multiple injections, which causes poor compliance and systemic side effects. Therefore, there is a need to improve delivery of therapeutic macromolecules to enable non-invasive delivery routes, less frequent dosing through controlled-release drug delivery, and improved drug targeting to increase efficacy and reduce side effects. Non-invasive administration routes such as intranasal, pulmonary, transdermal, ocular and oral delivery have been attempted intensively by formulating macromolecules into nanoparticulate carriers system such as polymeric and lipidic nanoparticles. This review discusses barriers to drug delivery and current formulation technologies to overcome the unfavorable properties of macromolecules via non-invasive delivery (mainly intranasal, pulmonary, transdermal oral and ocular) with a focus on nanoparticulate carrier systems. This review also provided a summary and discussion of recent data on non-invasive delivery of macromolecules using nanoparticulate formulations. Copyright© Bentham Science Publishers; For any queries, please email at

  9. The Origin and the Future of Macromolecule Ionization by Laser Irradiation

    Institute of Scientific and Technical Information of China (English)



    Starting in 1984,a Shimadzu Corporation Central Research Laboratory development team set about the challenge of developinng an instrument for analyzing macromolecules such as proteins. At the time, the conventional consensus among chemists was that, “laser ionization of macromolecules exceeding a molecular weight of 10,000 is imposible”. However, not being a chemist, I was unaware of this widelyheld belief.

  10. Organometallic macromolecules with piano stool coordination repeating units: chain configuration and stimulated solution behaviour. (United States)

    Cao, Kai; Ward, Jonathan; Amos, Ryan C; Jeong, Moon Gon; Kim, Kyoung Taek; Gauthier, Mario; Foucher, Daniel; Wang, Xiaosong


    Theoretical calculations illustrate that organometallic macromolecules with piano stool coordination repeating units (Fe-acyl complex) adopt linear chain configuration with a P-Fe-C backbone surrounded by aromatic groups. The macromolecules show molecular weight-dependent and temperature stimulated solution behaviour in DMSO.

  11. The flavonoid herbacetin diglucoside as a constituent of the lignan macromolecule from flaxseed hulls

    NARCIS (Netherlands)

    Struijs, K.; Vincken, J.P.; Verhoef, R.P.; Oostveen, van W.H.M.; Voragen, A.G.J.; Gruppen, H.


    Lignans in flaxseed are known to be part of a macromolecule in which they are connected through the linker-molecule hydroxy-methyl-glutaric acid (HMGA). In this study, the lignan macromolecule was extracted from flaxseed hulls and degraded to its monomeric constituents by complete saponification. Be

  12. Novel macromolecules derived from coumarin: synthesis and antioxidant activity (United States)

    Al-Amiery, Ahmed A.; Al-Majedy, Yasameen K.; Kadhum, Abdul Amir H.; Mohamad, Abu Bakar


    The rational design of 4-hydroxycoumarins with tailor-made antioxidant activities is required nowadays due to the wide variety of pharmacologically significant, structurally interesting of coumarins and researcher orientation toward green chemistry and natural products. A simple and unique coumarins have been achieved by reaction of 4-hydroxycoumarin with aromatic aldehyde accompanied with the creation of a macromolecules have 2-aminothiazolidin-4-one. The molecular structures of the compounds were characterized by the Fourier transformation infrared and Nuclear magnetic resonance spectroscopies, in addition to CHN analysis. The scavenging abilities of new compounds against stable DPPH radical (DPPH•) and hydrogen peroxide were done and the results show that the compounds exhibited high antioxidant activates.

  13. Surface induced self-organization of comb-like macromolecules (United States)

    Popov, Konstantin I; Palyulin, Vladimir V; Möller, Martin; Khokhlov, Alexei R


    Summary We present a review of the theoretical and experimental evidence for the peculiar properties of comb copolymers, demonstrating the uniqueness of these materials among other polymer architectures. These special properties include an increase in stiffness upon increasing side-chain length, the spontaneous curvature of adsorbed combs, rod–globule transition, and specific intramolecular self-assembly. We also propose a theory of chemically heterogeneous surface nanopattern formation in ultrathin films of comblike macromolecules containing two different types (A and B) of incompatible side chains (so-called binary combs). Side chains of the binary combs are strongly adsorbed on a surface and segregated with respect to the backbone. The thickness of surface domains formed by the B side chains is controlled by the interaction with the substrate. We predict the stability of direct and inverse disc-, torus- and stripelike nanostructures. Phase diagrams of the film are constructed. PMID:22003463

  14. Non-linear and non-local transport processes in heterogeneous media: from long-range correlated percolation to fracture and materials breakdown (United States)

    Sahimi, Muhammad


    We review and discuss recent progress in modelling non-linear and non-local transport processes in heterogeneous media. The non-locality that we consider is caused by long-range correlations that either exist in the morphology of the media, or are caused by the transport processes themselves. The interplay between the non-linearity and non-locality is discussed in depth with the aim of establishing that, often non-linearity and non-locality are “two sides of the same coin”, such that one may have no meaning without the presence of the other one. First, we discuss linear and scalar, but non-local transport processes and, in particular, consider those in percolation systems with long-range correlations. It appears that there are significant differences between percolative transport processes in which the long-range correlations (or the covariance function) decrease with the distance r between two points, and those in which they increase as r does. Application of this problem to flow and transport in geological formations is discussed. We then consider linear vector percolation, one type of which, the rigidity percolation, provides an example of a non-local vector transport in heterogeneous media. Applications of vector percolation to modelling elastic properties of glasses, composite solids and rock, mechanical and viscoelastic properties of polymers, and vibrations and dynamical properties of heterogeneous materials are discussed. Non-linear and non-local scalar transport processes are discussed next, including various breakdown phenomena in disordered composites, power-law transport, piecewise linear transport characterized by a threshold, and non-linear processes that arise as a result of imposing a large external potential gradient on a heterogeneous medium. Their relevance to flow of non-Newtonian fluids in porous media, to electrical currents and dielectric breakdown in composite solids and doped polycrystalline semiconductors, and several other problems is

  15. Supramolecular Chemistry and Mechanochemistry of Macromolecules: Recent Advances by Single-Molecule Force Spectroscopy. (United States)

    Cheng, Bo; Cui, Shuxun


    Atomic force spectroscopy (AFM)-based single-molecule force spectroscopy (SMFS) was invented in the 1990s. Since then, SMFS has been developed into a powerful tool to study the inter- and intra-molecular interactions of macromolecules. Using SMFS, a number of problems in the field of supramolecular chemistry and mechanochemistry have been studied at the single-molecule level, which are not accessible by traditional ensemble characterization methods. In this review, the principles of SMFS are introduced, followed by the discussion of several problems of contemporary interest at the interface of supramolecular chemistry and mechanochemistry of macromolecules, including single-chain elasticity of macromolecules, interactions between water and macromolecules, interactions between macromolecules and solid surface, and the interactions in supramolecular polymers.

  16. Nonlinear Maneuver Autopilot (United States)

    Menon, P. K. A.; Badgett, M. E.; Walker, R. A.


    Trajectory-control laws based on singular-perturbation theory and nonlinear dynamical modeling. Nonlinear maneuver autopilot commands flight-test trajectories of F-15 airplane. Underlying theory of controller enables separation of variables processed in fast and slow control loops, reducing amount of computation required.

  17. Nonlinear effects in optical fibers

    CERN Document Server

    Ferreira, Mario F


    Cutting-edge coverage of nonlinear phenomena occurring inside optical fibers Nonlinear fiber optics is a specialized part of fiber optics dealing with optical nonlinearities and their applications. As fiber-optic communication systems have become more advanced and complex, the nonlinear effects in optical fibers have increased in importance, as they adversely affect system performance. Paradoxically, the same nonlinear phenomena also offer the promise of addressing the bandwidth bottleneck for signal processing for future ultra-high speed optical networks. Nonlinear Effects in Optical Fiber

  18. In-Fiber Subpicosecond Pulse Shaping for Nonlinear Optical Telecommunication Data Processing at 640 Gbit/s

    DEFF Research Database (Denmark)

    Azaña, J.; Oxenløwe, Leif Katsuo; Palushani, Evarist


    -optic telecommunication links operating up to 640 Gbit/s. Experiments are presented demonstrating error-free 640-to-10 Gbit/s demultiplexing of the 64 tributary channels using the generated flat-top pulses for temporal gating in a Kerr-effect-based nonlinear optical loop mirror. The use of flat-top pulses has critical...

  19. Synthesis of biocontrol macromolecules by derivative of chitosan with surfactin and antifungal evaluation. (United States)

    Yuan, Bo; Xu, Pei-Yuan; Zhang, Yue-Ji; Wang, Pei-Pei; Yu, Hong; Jiang, Ji-Hong


    A derivative of chitosan was prepared with chitosan and β-cyclodextrins, which was synthesized by the immobilization reaction, as a carrier to adsorb surfactin produced from Bacillus amyloliquefaciens and got biological macromolecules. The antifungal activity against three sapstain fungi by a combination of macromolecules was tested. The results showed that the macromolecules inhibited the mycelium growth of sapstain fungi Lasiodiplodia rubropurpurea, L. crassispora, and L. theobromae by about 73.22%, 76.72%, and 70.22%, respectively. The macromolecules were relatively thermally stable with more than 50% of the antifungal activity even after being held at 121°C for 30 min. Meanwhile, the activity of the macromolecules remained more than 55% at a pH value ranging from 4 to 12. The macromolecules were resistant to hydrolysis by most protein-denaturing detergents and other enzymes. The results indicated the macromolecules might provide an alternative bioresource for the bio-control of sapstain. Copyright © 2014. Published by Elsevier B.V.

  20. Transpapillary (Nipple) Delivery of Macromolecules to the Breast: Proof of Concept Study. (United States)

    Dave, Kaushalkumar; Alsharif, Fahd M; Perumal, Omathanu


    Localized drug delivery to the breast can maximize drug concentration at the target site and minimize systemic drug distribution. To this end, the study explored the feasibility of delivering macromolecules to the breast through mammary papilla (nipple). The in vitro penetration of model macromolecules (inulin, dextran, ovalbumin, and bovine serum albumin) varying in molecular weight from 5 to 67 kDa was studied using excised porcine and human mammary papilla. The penetration of macromolecules decreased with increase in molecular weight. The penetration of the macromolecules was significantly higher through the mammary papilla in comparison to breast skin. In vitro penetration of the macromolecules was similar in human and porcine mammary papilla. Iontophoresis was used to enhance the transport of bovine serum albumin (BSA) through the mammary papilla. The flux and cumulative amount permeated was increased by 2- to 4-fold by iontophoresis. The macromolecules were transported through the ducts and the surrounding connective tissue in the mammary papilla. Overall, the results from this study for the first time demonstrate the feasibility of delivering macromolecules through the mammary papilla. These findings have implications for developing safe and effective localized therapeutic approaches for breast cancer.

  1. Multiscale geometric modeling of macromolecules I: Cartesian representation. (United States)

    Xia, Kelin; Feng, Xin; Chen, Zhan; Tong, Yiying; Wei, Guo Wei


    This paper focuses on the geometric modeling and computational algorithm development of biomolecular structures from two data sources: Protein Data Bank (PDB) and Electron Microscopy Data Bank (EMDB) in the Eulerian (or Cartesian) representation. Molecular surface (MS) contains non-smooth geometric singularities, such as cusps, tips and self-intersecting facets, which often lead to computational instabilities in molecular simulations, and violate the physical principle of surface free energy minimization. Variational multiscale surface definitions are proposed based on geometric flows and solvation analysis of biomolecular systems. Our approach leads to geometric and potential driven Laplace-Beltrami flows for biomolecular surface evolution and formation. The resulting surfaces are free of geometric singularities and minimize the total free energy of the biomolecular system. High order partial differential equation (PDE)-based nonlinear filters are employed for EMDB data processing. We show the efficacy of this approach in feature-preserving noise reduction. After the construction of protein multiresolution surfaces, we explore the analysis and characterization of surface morphology by using a variety of curvature definitions. Apart from the classical Gaussian curvature and mean curvature, maximum curvature, minimum curvature, shape index, and curvedness are also applied to macromolecular surface analysis for the first time. Our curvature analysis is uniquely coupled to the analysis of electrostatic surface potential, which is a by-product of our variational multiscale solvation models. As an expository investigation, we particularly emphasize the numerical algorithms and computational protocols for practical applications of the above multiscale geometric models. Such information may otherwise be scattered over the vast literature on this topic. Based on the curvature and electrostatic analysis from our multiresolution surfaces, we introduce a new concept, the

  2. Focus issue introduction: nonlinear optics 2013. (United States)

    Dadap, Jerry I; Karlsson, Magnus; Panoiu, Nicolae C


    Nonlinear Optics has continued to develop over the last few years at an extremely fast pace, with significant advances being reported in nonlinear optical metamaterials, optical signal processing, quantum optics, nonlinear optics at subwavelength scale, and biophotonics. These exciting new developments have generated significant potential for a broad spectrum of technological applications in which nonlinear-optical processes play a central role.

  3. Fault Detection for Nonlinear Systems

    DEFF Research Database (Denmark)

    Stoustrup, Jakob; Niemann, H.H.


    The paper describes a general method for designing fault detection and isolation (FDI) systems for nonlinear processes. For a rich class of nonlinear systems, a nonlinear FDI system can be designed using convex optimization procedures. The proposed method is a natural extension of methods based...

  4. Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging. (United States)

    Pliss, Artem; Peng, Xiao; Liu, Lixin; Kuzmin, Andrey; Wang, Yan; Qu, Junle; Li, Yuee; Prasad, Paras N


    Molecular organization of a cell is dynamically transformed along the course of cellular physiological processes, pathologic developments or derived from interactions with drugs. The capability to measure and monitor concentrations of macromolecules in a single cell would greatly enhance studies of cellular processes in heterogeneous populations. In this communication, we introduce and experimentally validate a bio-analytical single-cell assay, wherein the overall concentration of macromolecules is estimated in specific subcellular domains, such as structure-function compartments of the cell nucleus as well as in nucleoplasm. We describe quantitative mapping of local biomolecular concentrations, either intrinsic relating to the functional and physiological state of a cell, or altered by a therapeutic drug action, using two-photon excited fluorescence lifetime imaging (FLIM). The proposed assay utilizes a correlation between the fluorescence lifetime of fluorophore and the refractive index of its microenvironment varying due to changes in the concentrations of macromolecules, mainly proteins. Two-photon excitation in Near-Infra Red biological transparency window reduced the photo-toxicity in live cells, as compared with a conventional single-photon approach. Using this new assay, we estimated average concentrations of proteins in the compartments of nuclear speckles and in the nucleoplasm at ~150 mg/ml, and in the nucleolus at ~284 mg/ml. Furthermore, we show a profound influence of pharmaceutical inhibitors of RNA synthesis on intracellular protein density. The approach proposed here will significantly advance theranostics, and studies of drug-cell interactions at the single-cell level, aiding development of personal molecular medicine.

  5. Physical Delivery of Macromolecules using High-Aspect Ratio Nanostructured Materials. (United States)

    Lee, Kunwoo; Lingampalli, Nithya; Pisano, Albert P; Murthy, Niren; So, Hongyun


    There is great need for the development of an efficient delivery method of macromolecules, including nucleic acids, proteins, and peptides, to cell cytoplasm without eliciting toxicity or changing cell behavior. High-aspect ratio nanomaterials have addressed many challenges present in conventional methods, such as cell membrane passage and endosomal degradation, and have shown the feasibility of efficient high-throughput macromolecule delivery with minimal perturbation of cells. This review describes the recent advances of in vitro and in vivo physical macromolecule delivery with high-aspect ratio nanostructured materials and summarizes the synthesis methods, material properties, relevant applications, and various potential directions.

  6. Nonlinear optics

    CERN Document Server

    Bloembergen, Nicolaas


    Nicolaas Bloembergen, recipient of the Nobel Prize for Physics (1981), wrote Nonlinear Optics in 1964, when the field of nonlinear optics was only three years old. The available literature has since grown by at least three orders of magnitude.The vitality of Nonlinear Optics is evident from the still-growing number of scientists and engineers engaged in the study of new nonlinear phenomena and in the development of new nonlinear devices in the field of opto-electronics. This monograph should be helpful in providing a historical introduction and a general background of basic ideas both for expe

  7. Interesting effect of aspect ratio on Kerr nonlinear optical processes in subwavelength diameter single-mode trapezoidal index fiber using Marcuse-type spot-size formulations (United States)

    Sadhu, Arunangshu; Sarkar, Somenath


    We investigate the Kerr nonlinear optical processes (NOPs) in the case of a single-mode trapezoidal index fiber based on recently formulated and appropriate Marcuse-type relations for spot size in terms of normalized frequency corresponding to such fiber having various aspect ratios. With the help of these relations, we have analyzed the maximum NOP in these fibers having prospective the merits of tight light confinement in the subwavelength diameter waveguiding region. The comparative investigation reveals that the aspect ratio having a value of 0.7 is the most promising candidate for maximum optical nonlinearity, constructional convenience, and less diffraction. The analysis should be attractive for system users as a ready reference.

  8. Channel-Forming Bacterial Toxins in Biosensing and Macromolecule Delivery

    Directory of Open Access Journals (Sweden)

    Philip A. Gurnev


    Full Text Available To intoxicate cells, pore-forming bacterial toxins are evolved to allow for the transmembrane traffic of different substrates, ranging from small inorganic ions to cell-specific polypeptides. Recent developments in single-channel electrical recordings, X-ray crystallography, protein engineering, and computational methods have generated a large body of knowledge about the basic principles of channel-mediated molecular transport. These discoveries provide a robust framework for expansion of the described principles and methods toward use of biological nanopores in the growing field of nanobiotechnology. This article, written for a special volume on “Intracellular Traffic and Transport of Bacterial Protein Toxins”, reviews the current state of applications of pore-forming bacterial toxins in small- and macromolecule-sensing, targeted cancer therapy, and drug delivery. We discuss the electrophysiological studies that explore molecular details of channel-facilitated protein and polymer transport across cellular membranes using both natural and foreign substrates. The review focuses on the structurally and functionally different bacterial toxins: gramicidin A of Bacillus brevis, α-hemolysin of Staphylococcus aureus, and binary toxin of Bacillus anthracis, which have found their “second life” in a variety of developing medical and technological applications.

  9. Channel-forming bacterial toxins in biosensing and macromolecule delivery. (United States)

    Gurnev, Philip A; Nestorovich, Ekaterina M


    To intoxicate cells, pore-forming bacterial toxins are evolved to allow for the transmembrane traffic of different substrates, ranging from small inorganic ions to cell-specific polypeptides. Recent developments in single-channel electrical recordings, X-ray crystallography, protein engineering, and computational methods have generated a large body of knowledge about the basic principles of channel-mediated molecular transport. These discoveries provide a robust framework for expansion of the described principles and methods toward use of biological nanopores in the growing field of nanobiotechnology. This article, written for a special volume on "Intracellular Traffic and Transport of Bacterial Protein Toxins", reviews the current state of applications of pore-forming bacterial toxins in small- and macromolecule-sensing, targeted cancer therapy, and drug delivery. We discuss the electrophysiological studies that explore molecular details of channel-facilitated protein and polymer transport across cellular membranes using both natural and foreign substrates. The review focuses on the structurally and functionally different bacterial toxins: gramicidin A of Bacillus brevis, α-hemolysin of Staphylococcus aureus, and binary toxin of Bacillus anthracis, which have found their "second life" in a variety of developing medical and technological applications.

  10. Graphical Methods for Quantifying Macromolecules through Bright Field Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Hang; DeFilippis, Rosa Anna; Tlsty, Thea D.; Parvin, Bahram


    Bright ?eld imaging of biological samples stained with antibodies and/or special stains provides a rapid protocol for visualizing various macromolecules. However, this method of sample staining and imaging is rarely employed for direct quantitative analysis due to variations in sample fixations, ambiguities introduced by color composition, and the limited dynamic range of imaging instruments. We demonstrate that, through the decomposition of color signals, staining can be scored on a cell-by-cell basis. We have applied our method to Flbroblasts grown from histologically normal breast tissue biopsies obtained from two distinct populations. Initially, nuclear regions are segmented through conversion of color images into gray scale, and detection of dark elliptic features. Subsequently, the strength of staining is quanti?ed by a color decomposition model that is optimized by a graph cut algorithm. In rare cases where nuclear signal is significantly altered as a result of samplepreparation, nuclear segmentation can be validated and corrected. Finally, segmented stained patterns are associated with each nuclear region following region-based tessellation. Compared to classical non-negative matrix factorization, proposed method (i) improves color decomposition, (ii) has a better noise immunity, (iii) is more invariant to initial conditions, and (iv) has a superior computing performance

  11. Quantification of the Molecular Topology for Hierarchical Macromolecules (United States)

    Beaucage, Gregory


    Hierarchical structures are often produced from ramified macromolecules such as comb, star, hyperbranched and dendritic polymers. We have recently derived a method for the description of complex molecular and nanostructural topologies based on a statistical analysis [1,2]. The method has been applied to a wide range of hierarchical materials from long chain branched polyolefins, hyperbranched polymers [3], star polymers, H-branched polymers to cyclics, biopolymers [4], and branched nanostructured aggregates. This method, when applied to neutron scattering data, yields the mole fraction of a structure involved in branching, the number of branch sites, the average branch length, and the number if inner chain segments. Further, quantitative measures of the convolution or tortuosity of the structure and the connectivity of the branching network can be made, opening a new window for our understanding of complex molecular topologies. This understanding has recently been applied to biological chain molecules to understand protein and RNA folding [4] for example as well as to aggregated, nanostructured, carbon soot. [0pt] [1] Beaucage, G, Phys. Rev. E 2004, 70, 031401. [2] Kulkarni, AS & Beaucage, G, J. Polym. Sci. Part B: Polym. Phys. 2006, 44, 1395. [3] Kulkarni, AS & Beaucage, G, Macromol. Rapid Comm. 2007, 28, 1312.?4) Beaucage, G, Biophysical J. 2008, 95, 503.

  12. 基于核PLS方法的非线性过程在线监控%Online nonlinear process monitoring using kernel partial least squares

    Institute of Scientific and Technical Information of China (English)

    胡益; 王丽; 马贺贺; 侍洪波


    针对过程监控数据的非线性特点,提出了一种基于核偏最小二乘(KPLS)的监控方法.KPLS方法是将原始输入数据通过核函数映射到高维特征空间,然后在高维特征空间再进行偏最小二乘(PLS)运算.与线性PIS相比,KPLS方法能充分利用样本空间信息,建立起输入输出变量之间的非线性关系.与其他非线性PLS方法不同,KPLS方法只需要进行线性运算,从而避免非线性优化问题.在对过程进行监控时,首先采用KPLS方法建立模型,得到得分向量,然后计算出T2和SPE统计量及其相应的控制限.Tennessee Eastman (TE)模型上的仿真研究结果表明,所提方法比线性PLS方法具有更好的过程监控性能.%To handle the nonlinear problem for process monitoring, a new technique based on kernel partial least squares (KPLS) is developed. KPLS is an improved partial least squares (PLS) method, and its main idea is to first map the input space into a high-dimensional feature space via a nonlinear kernel function and then to use the standard PLS in that feature space. Compared to linear PLS, KPLS can make full use of the sample space information, and effectively capture the nonlinear relationship between input variables and output variables. Different from other nonlinear PLS, KPLS requires only linear algebra and does not involve any nonlinear optimization. For process data, firstly KPLS was used to derive regression model and got the score vectors, and then two statistics, T2 and SPE, and corresponding control limits were calculated. A case study of the Tennessee-Eastman (TE) process illustrated that the proposed approach showed superior process monitoring performance compared to linear PLS.

  13. A simple model for electrical charge in globular macromolecules and linear polyelectrolytes in solution (United States)

    Krishnan, M.


    We present a model for calculating the net and effective electrical charge of globular macromolecules and linear polyelectrolytes such as proteins and DNA, given the concentration of monovalent salt and pH in solution. The calculation is based on a numerical solution of the non-linear Poisson-Boltzmann equation using a finite element discretized continuum approach. The model simultaneously addresses the phenomena of charge regulation and renormalization, both of which underpin the electrostatics of biomolecules in solution. We show that while charge regulation addresses the true electrical charge of a molecule arising from the acid-base equilibria of its ionizable groups, charge renormalization finds relevance in the context of a molecule's interaction with another charged entity. Writing this electrostatic interaction free energy in terms of a local electrical potential, we obtain an "interaction charge" for the molecule which we demonstrate agrees closely with the "effective charge" discussed in charge renormalization and counterion-condensation theories. The predictions of this model agree well with direct high-precision measurements of effective electrical charge of polyelectrolytes such as nucleic acids and disordered proteins in solution, without tunable parameters. Including the effective interior dielectric constant for compactly folded molecules as a tunable parameter, the model captures measurements of effective charge as well as published trends of pKa shifts in globular proteins. Our results suggest a straightforward general framework to model electrostatics in biomolecules in solution. In offering a platform that directly links theory and experiment, these calculations could foster a systematic understanding of the interrelationship between molecular 3D structure and conformation, electrical charge and electrostatic interactions in solution. The model could find particular relevance in situations where molecular crystal structures are not available or

  14. Nonlinear Dynamics

    Institute of Scientific and Technical Information of China (English)


    3.1 A Unified Nonlinear Feedback Functional Method for Study Both Control and Synchronization of Spatiotemporal Chaos Fang Jinqing Ali M. K. (Department of Physics, The University of Lethbridge,Lethbridge, Alberta T1K 3M4,Canada) Two fundamental questions dominate future chaos control theories.The first is the problem of controlling hyperchaos in higher dimensional systems.The second question has yet to be addressed:the problem of controlling spatiotemporal chaos in a spatiotemporal system.In recent years, control and synchronization of spatiotemporal chaos and hyperchaos have became a much more important and challenging subject. The reason for this is the control and synchronism of such behaviours have extensive and great potential of interdisciplinary applications, such as security communication, information processing, medicine and so on. However, this subject is not much known and remains an outstanding open.

  15. Nonlinear processes associated with the amplification of MHz-linewidth laser pulses in single-mode Tm:fiber (United States)

    Sincore, Alex; Bodnar, Nathan; Bradford, Joshua; Abdulfattah, Ali; Shah, Lawrence; Richardson, Martin C.


    This work studies the accumulated nonlinearities when amplifying a narrow linewidth 2053 nm seed in a single mode Tm:fiber amplifier. A control of repetition rate and pulse duration (>30 ns). The pulses are subsequently amplified and the repetition rate is further reduced using a second acousto-optic modulator. It is well known that spectral degradation occurs in such fibers for peak powers over 100's of watts due to self-phase modulation, four-wave mixing, and stimulated Raman scattering. In addition to enabling a thorough test bed to study such spectral broadening, this system will also enable the investigation of stimulated Brillouin scattering thresholds in the same system. This detailed study of the nonlinearities encountered in 2 μm fiber amplifiers is important in a range of applications from telecommunications to the amplification of ultrashort laser pulses.

  16. Experimental study of nonlinear processes in a swept-wing boundary layer at the mach number M=2 (United States)

    Yermolaev, Yu. G.; Kosinov, A. D.; Semionov, N. V.


    Results of experiments aimed at studying the linear and nonlinear stages of the development of natural disturbances in the boundary layer on a swept wing at supersonic velocities are presented. The experiments are performed on a swept wing model with a lens-shaped airfoil, leading-edge sweep angle of 45°, and relative thickness of 3%. The disturbances in the flow are recorded by a constant-temperature hot-wire anemometer. For determining the nonlinear interaction of disturbances, the kurtosis and skewness are estimated for experimentally obtained distributions of the oscillating signal over the streamwise coordinate or along the normal to the surface. The disturbances are found to increase in the frequency range from 8 to 35 kHz in the region of their linear development, whereas enhancement of high-frequency disturbances is observed in the region of their nonlinear evolution. It is demonstrated that the growth of disturbances in the high-frequency spectral range ( f > 35 kHz) is caused by the secondary instability.

  17. Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules. (United States)

    Terasawa, Hidetoshi; Nishimura, Kazuya; Suzuki, Hiroaki; Matsuura, Tomoaki; Yomo, Tetsuya


    Mechanisms that enabled primitive cell membranes to self-reproduce have been discussed based on the physicochemical properties of fatty acids; however, there must be a transition to modern cell membranes composed of phospholipids [Budin I, Szostak JW (2011) Proc Natl Acad Sci USA 108:5249-5254]. Thus, a growth-division mechanism of membranes that does not depend on the chemical nature of amphiphilic molecules must have existed. Here, we show that giant unilamellar vesicles composed of phospholipids can undergo the coupled process of fusion and budding transformation, which mimics cell growth and division. After gaining excess membrane by electrofusion, giant vesicles spontaneously transform into the budded shape only when they contain macromolecules (polymers) inside their aqueous core. This process is a result of the vesicle maximizing the translational entropy of the encapsulated polymers (depletion volume effect). Because the cell is a lipid membrane bag containing highly concentrated biopolymers, this coupling process that is induced by physical and nonspecific interactions may have a general importance in the self-reproduction of the early cellular compartments.

  18. Electric Birefringence: A Simple Apparatus for Determining Physical Parameters of Macromolecules and Colloids. (United States)

    Trimm, Harold H.; And Others


    Describes a birefringence apparatus that can be assembled for less than $100 and can be used to measure both the dimensions and dipole moments of many macromolecules. Details are given of the construction and manipulation of the apparatus. (JN)

  19. Development of modified release gliclazide biological macromolecules using natural biodegradable polymers. (United States)

    Prajapati, Vipulkumar D; Mashru, Krupa H; Solanki, Himanshu K; Jani, Girish K


    Modified release biological macromolecules (beads) of gliclazide using sodium alginate combined with either gellan gum or pectin in different ratios were prepared by Ionotropic gelation method. Biological macromolecules were evaluated for different physico-chemical parameters. Increase in polymers proportion showed difficulty in production of biological macromolecules due to high viscosity of dispersion. As the polymer concentration increases, the swelling and entrapment efficiency of drug increased. Compared to all other batches and commercial modified release gliclazide tablet, formulated biological macromolecules of sodium alginate with pectin (2:1 ratio) and with gellan gum (6:0.75 ratio) exhibited spherical shape, biphasic in vitro release profile and initial high drug release followed by moderate release up to 12 h as matrix diffusion kinetics and Higuchi model as well as Korsmeyer model. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. solveME: fast and reliable solution of nonlinear ME models

    DEFF Research Database (Denmark)

    Yang, Laurence; Ma, Ding; Ebrahim, Ali


    reconstructions (M models), are multiscale, and growth maximization is a nonlinear programming (NLP) problem, mainly due to macromolecule dilution constraints. Results: Here, we address these computational challenges. We develop a fast and numerically reliable solution method for growth maximization in ME models...

  1. Insights and Lessons from a Scientific Conference on Non-Invasive Delivery of Macromolecules. (United States)

    Savla, Ronak; Mrsny, Randall J; Park, Kinam; Aubert, Isabelle; Stamoran, Cornell


    A growing share of the pharmaceutical development pipeline is occupied by macromolecule drugs, which are primarily administered by injection. Despite decades of attempts, non-invasive delivery of macromolecules has seen only a few success stories. Potential benefits of non-invasive administration include better patient acceptance and adherence and potentially better efficacy and safety. Greater inter-disciplinary dialogue and collaboration are integral to realizing these benefits.

  2. Thermochemolysis: A New Sample Preparation Approach for the Detection of Organic Components of Complex Macromolecules in Mars Rocks via Gas Chromatography Mass Spectrometry in SAM on MSL (United States)

    Eugenbrode, J.; Glavin, D.; Dworkin, J.; Conrad, P.; Mahaffy, P.


    Organic chemicals, when present in extraterrestrial samples, afford precious insight into past and modern conditions elsewhere in the Solar System . No single technology identifies all molecular components because naturally occurring molecules have different chemistries (e.g., polar vs. non-polar, low to high molecular weight) and interface with the ambient sample chemistry in a variety of modes (i.e., organics may be bonded, absorbed or trapped by minerals, liquids, gases, or other organics). More than 90% of organic matter in most natural samples on Earth and in meteorites is composed of complex macromolecules (e.g. biopolymers, complex biomolecules, humic substances, kerogen) because the processes that tend to break down organic molecules also tend towards complexation of the more recalcitrant components. Thus, methodologies that tap the molecular information contained within macromolecules may be critical to detecting extraterrestrial organic matter and assessing the sources and processes influencing its nature.

  3. A Comparative Study of Applying Active-Set and Interior Point Methods in MPC for Controlling Nonlinear pH Process

    Directory of Open Access Journals (Sweden)

    Syam Syafiie


    Full Text Available A comparative study of Model Predictive Control (MPC using active-set method and interior point methods is proposed as a control technique for highly non-linear pH process. The process is a strong acid-strong base system. A strong acid of hydrochloric acid (HCl and a strong base of sodium hydroxide (NaOH with the presence of buffer solution sodium bicarbonate (NaHCO3 are used in a neutralization process flowing into reactor. The non-linear pH neutralization model governed in this process is presented by multi-linear models. Performance of both controllers is studied by evaluating its ability of set-point tracking and disturbance-rejection. Besides, the optimization time is compared between these two methods; both MPC shows the similar performance with no overshoot, offset, and oscillation. However, the conventional active-set method gives a shorter control action time for small scale optimization problem compared to MPC using IPM method for pH control.

  4. Nonlinear system identification by Gustafson-Kessel fuzzy clustering and supervised local model network learning for the drug absorption spectra process. (United States)

    Teslic, Luka; Hartmann, Benjamin; Nelles, Oliver; Skrjanc, Igor


    This paper deals with the problem of fuzzy nonlinear model identification in the framework of a local model network (LMN). A new iterative identification approach is proposed, where supervised and unsupervised learning are combined to optimize the structure of the LMN. For the purpose of fitting the cluster-centers to the process nonlinearity, the Gustafsson-Kessel (GK) fuzzy clustering, i.e., unsupervised learning, is applied. In combination with the LMN learning procedure, a new incremental method to define the number and the initial locations of the cluster centers for the GK clustering algorithm is proposed. Each data cluster corresponds to a local region of the process and is modeled with a local linear model. Since the validity functions are calculated from the fuzzy covariance matrices of the clusters, they are highly adaptable and thus the process can be described with a very sparse amount of local models, i.e., with a parsimonious LMN model. The proposed method for constructing the LMN is finally tested on a drug absorption spectral process and compared to two other methods, namely, Lolimot and Hilomot. The comparison between the experimental results when using each method shows the usefulness of the proposed identification algorithm.

  5. The flavonoid herbacetin diglucoside as a constituent of the lignan macromolecule from flaxseed hulls. (United States)

    Struijs, Karin; Vincken, Jean-Paul; Verhoef, René; van Oostveen-van Casteren, Willemiek H M; Voragen, Alphons G J; Gruppen, Harry


    Lignans in flaxseed are known to be part of a macromolecule in which they are connected through the linker-molecule hydroxy-methyl-glutaric acid (HMGA). In this study, the lignan macromolecule was extracted from flaxseed hulls and degraded to its monomeric constituents by complete saponification. Besides secoisolariciresinol diglucoside (SDG), the phenolic compounds p-coumaric acid glucoside (CouAG) and ferulic acid glucoside (FeAG) were isolated, which was expected based on indications from the literature. Also the flavonoid herbacetin diglucoside (HDG) was found. The presence of HDG was confirmed by NMR following preparative RP-HPLC purification. Also the presence of the three other constituents (CouAG, FeAG and SDG) was confirmed by NMR. To prove that HDG is a substructure of the lignan macromolecule, the macromolecule was fragmented by partial saponification. A fragment consisting of HDG and HMGA was indicated. This fragment was isolated by preparative RP-HPLC and its identity was confirmed by NMR. It is concluded that the flavonoid HDG is a substructure of the lignan macromolecule from flaxseed hulls and that it is incorporated in the macromolecule via the same linker-molecule as SDG.

  6. Practical Nonlinearities (United States)


    Advanced Research Projects Agency (DARPA) Dynamics-Enabled Frequency Sources (DEFYS) program is focused on the convergence of nonlinear dynamics and...Early work in this program has shown that nonlinear dynamics can provide performance advantages. However, the pathway from initial results to...dependent nonlinear stiffness observed in these devices. This work is ongoing, and will continue through the final period of this program . Reference 9

  7. Nonlinear oscillations

    CERN Document Server

    Nayfeh, Ali Hasan


    Nonlinear Oscillations is a self-contained and thorough treatment of the vigorous research that has occurred in nonlinear mechanics since 1970. The book begins with fundamental concepts and techniques of analysis and progresses through recent developments and provides an overview that abstracts and introduces main nonlinear phenomena. It treats systems having a single degree of freedom, introducing basic concepts and analytical methods, and extends concepts and methods to systems having degrees of freedom. Most of this material cannot be found in any other text. Nonlinear Oscillations uses sim

  8. Nonlinear Science

    CERN Document Server

    Yoshida, Zensho


    This book gives a general, basic understanding of the mathematical structure "nonlinearity" that lies in the depths of complex systems. Analyzing the heterogeneity that the prefix "non" represents with respect to notions such as the linear space, integrability and scale hierarchy, "nonlinear science" is explained as a challenge of deconstruction of the modern sciences. This book is not a technical guide to teach mathematical tools of nonlinear analysis, nor a zoology of so-called nonlinear phenomena. By critically analyzing the structure of linear theories, and cl

  9. Nonlinear analysis

    CERN Document Server

    Nanda, Sudarsan


    "Nonlinear analysis" presents recent developments in calculus in Banach space, convex sets, convex functions, best approximation, fixed point theorems, nonlinear operators, variational inequality, complementary problem and semi-inner-product spaces. Nonlinear Analysis has become important and useful in the present days because many real world problems are nonlinear, nonconvex and nonsmooth in nature. Although basic concepts have been presented here but many results presented have not appeared in any book till now. The book could be used as a text for graduate students and also it will be useful for researchers working in this field.

  10. A new automated assessment method for contrast-detail images by applying support vector machine and its robustness to nonlinear image processing. (United States)

    Takei, Takaaki; Ikeda, Mitsuru; Imai, Kuniharu; Yamauchi-Kawaura, Chiyo; Kato, Katsuhiko; Isoda, Haruo


    The automated contrast-detail (C-D) analysis methods developed so-far cannot be expected to work well on images processed with nonlinear methods, such as noise reduction methods. Therefore, we have devised a new automated C-D analysis method by applying support vector machine (SVM), and tested for its robustness to nonlinear image processing. We acquired the CDRAD (a commercially available C-D test object) images at a tube voltage of 120 kV and a milliampere-second product (mAs) of 0.5-5.0. A partial diffusion equation based technique was used as noise reduction method. Three radiologists and three university students participated in the observer performance study. The training data for our SVM method was the classification data scored by the one radiologist for the CDRAD images acquired at 1.6 and 3.2 mAs and their noise-reduced images. We also compared the performance of our SVM method with the CDRAD Analyser algorithm. The mean C-D diagrams (that is a plot of the mean of the smallest visible hole diameter vs. hole depth) obtained from our devised SVM method agreed well with the ones averaged across the six human observers for both original and noise-reduced CDRAD images, whereas the mean C-D diagrams from the CDRAD Analyser algorithm disagreed with the ones from the human observers for both original and noise-reduced CDRAD images. In conclusion, our proposed SVM method for C-D analysis will work well for the images processed with the non-linear noise reduction method as well as for the original radiographic images.

  11. Experimental study on a nonlinear photonics process of Er(0.5)Yb(3):FOV oxyfluoride nanophase vitroceramics (United States)

    Chen, Xiaobo; Song, Zengfu; Hu, Lili; Zhang, Junjie; Wen, Lei


    We study the nonlinear photonics of rare-earth-doped oxyfluoride nanophase vitroceramics (FOV), oxyfluoride glass (FOG), and ZBLAN fluoride glass. We found that an interesting fluorescence intensity inversion phenomenon between red and green fluorescence occurs from Er(0.5)Yb(3):FOV. The dynamic range ∑ of the intensity inversion between red and green fluorescence of Er(0.5)Yb(3):FOV is about 5.753×102, which is 100 to 1000 times larger than those of other materials. One of the applications of this phenomenon is double-wavelength fluorescence falsification-preventing technology, which is proved to possess the novel antifriction loss and antiscribble properties.

  12. Perspective: On the importance of hydrodynamic interactions in the subcellular dynamics of macromolecules (United States)

    Skolnick, Jeffrey


    An outstanding challenge in computational biophysics is the simulation of a living cell at molecular detail. Over the past several years, using Stokesian dynamics, progress has been made in simulating coarse grained molecular models of the cytoplasm. Since macromolecules comprise 20%-40% of the volume of a cell, one would expect that steric interactions dominate macromolecular diffusion. However, the reduction in cellular diffusion rates relative to infinite dilution is due, roughly equally, to steric and hydrodynamic interactions, HI, with nonspecific attractive interactions likely playing rather a minor role. HI not only serve to slow down long time diffusion rates but also cause a considerable reduction in the magnitude of the short time diffusion coefficient relative to that at infinite dilution. More importantly, the long range contribution of the Rotne-Prager-Yamakawa diffusion tensor results in temporal and spatial correlations that persist up to microseconds and for intermolecular distances on the order of protein radii. While HI slow down the bimolecular association rate in the early stages of lipid bilayer formation, they accelerate the rate of large scale assembly of lipid aggregates. This is suggestive of an important role for HI in the self-assembly kinetics of large macromolecular complexes such as tubulin. Since HI are important, questions as to whether continuum models of HI are adequate as well as improved simulation methodologies that will make simulations of more complex cellular processes practical need to be addressed. Nevertheless, the stage is set for the molecular simulations of ever more complex subcellular processes. PMID:27634243

  13. Film Self-Assembly of Oppositely Charged Macromolecules Triggered by Electrochemistry through a Morphogenic Approach. (United States)

    Dochter, Alexandre; Garnier, Tony; Pardieu, Elodie; Chau, Nguyet Trang Thanh; Maerten, Clément; Senger, Bernard; Schaaf, Pierre; Jierry, Loïc; Boulmedais, Fouzia


    The development of new surface functionalization methods that are easy to use, versatile, and allow local deposition represents a real scientific challenge. Overcoming this challenge, we present here a one-pot process that consists in self-assembling, by electrochemistry on an electrode, films made of oppositely charged macromolecules. This method relies on a charge-shifting polyanion, dimethylmaleic-modified poly(allylamine) (PAHd), that undergoes hydrolysis at acidic pH, leading to an overall switching of its charge. When a mixture of the two polyanions, PAHd and poly(styrenesulfonate) (PSS), is placed in contact with an electrode, where the pH is decreased locally by electrochemistry, the transformation of PAHd into a polycation (PAH) leads to the continuous self-assembly of a nanometric PAH/PSS film by electrostatic interactions. The pH decrease is obtained by the electrochemical oxidation of hydroquinone, which produces protons locally over nanometric distances. Using a negatively charged enzyme, alkaline phosphatase (AP), instead of PSS, this one-pot process allows the creation of enzymatically active films. Under mild conditions, self-assembled PAH/AP films have an enzymatic activity which is adjustable simply by controlling the self-assembly time. The selective functionalization of microelectrode arrays by PAH/AP was achieved, opening the route toward miniaturized biosensors.

  14. Electrospun ECM macromolecules as biomimetic scaffold for regenerative medicine: challenges for preserving conformation and bioactivity

    Directory of Open Access Journals (Sweden)

    Chiara Emma Campiglio


    Full Text Available The extracellular matrix (ECM, the physiological scaffold for cells in vivo, provides structural support to cells and guaranties tissue integrity. At the same time, however, it represents an extremely complex and finely tuned signaling environment that contributes in regulating tissue homeostasis and repair. ECM can bind, release and activate signaling molecules and also modulate cell reaction to soluble factors. Cell-ECM interactions, as a result, are recognized to be critical for physiological wound healing, and consequently in guiding regeneration. Due to its complexity, mimicking ECM chemistry and architecture appears a straightforward strategy to exploit the benefits of a biologically recognizable and cell-instructive environment. As ECM consists primarily of sub-micrometric fibers, electrospinning, a simple and versatile technique, has attracted the majority efforts aimed at reprocessing of biologically occurring molecules. However, the ability to trigger specific cellular behavior is likely to depend on both the chemical and conformational properties of biological molecules. As a consequence, when ECM macromolecules are electrospun, investigating the effect of processing on their structure, and the extent to which their potential in directing cellular behavior is preserved, appears crucial. In this perspective, this review explores the electrospinning of ECM molecules specifically focusing on the effect of processing on polymer structure and on in vitro or in vivo experiments designed to confirm the maintenance of their instructive role.

  15. Nonlinear Stokes Mueller Polarimetry

    CERN Document Server

    Samim, Masood; Barzda, Virginijus


    The Stokes Mueller polarimetry is generalized to include nonlinear optical processes such as second- and third-harmonic generation, sum- and difference-frequency generations. The overall algebraic form of the polarimetry is preserved, where the incoming and outgoing radiations are represented by column vectors and the intervening medium is represented by a matrix. Expressions for the generalized nonlinear Stokes vector and the Mueller matrix are provided in terms of coherency and correlation matrices, expanded by higher-dimensional analogues of Pauli matrices. In all cases, the outgoing radiation is represented by the conventional $4\\times 1$ Stokes vector, while dimensions of the incoming radiation Stokes vector and Mueller matrix depend on the order of the process being examined. In addition, relation between nonlinear susceptibilities and the measured Mueller matrices are explicitly provided. Finally, the approach of combining linear and nonlinear optical elements is discussed within the context of polarim...

  16. A closure relation to molecular theory of solvation for macromolecules (United States)

    Kobryn, Alexander E.; Gusarov, Sergey; Kovalenko, Andriy


    We propose a closure to the integral equations of molecular theory of solvation, particularly suitable for polar and charged macromolecules in electrolyte solution. This includes such systems as oligomeric polyelectrolytes at a finite concentration in aqueous and various non-aqueous solutions, as well as drug-like compounds in solution. The new closure by Kobryn, Gusarov, and Kovalenko (KGK closure) imposes the mean spherical approximation (MSA) almost everywhere in the solvation shell but levels out the density distribution function to zero (with the continuity at joint boundaries) inside the repulsive core and in the spatial regions of strong density depletion emerging due to molecular associative interactions. Similarly to MSA, the KGK closure reduces the problem to a linear equation for the direct correlation function which is predefined analytically on most of the solvation shells and has to be determined numerically on a relatively small (three-dimensional) domain of strong depletion, typically within the repulsive core. The KGK closure leads to the solvation free energy in the form of the Gaussian fluctuation (GF) functional. We first test the performance of the KGK closure coupled to the reference interaction site model (RISM) integral equations on the examples of Lennard-Jones liquids, polar and nonpolar molecular solvents, including water, and aqueous solutions of simple ions. The solvation structure, solvation chemical potential, and compressibility obtained from RISM with the KGK closure favorably compare to the results of the hypernetted chain (HNC) and Kovalenko-Hirata (KH) closures, including their combination with the GF solvation free energy. We then use the KGK closure coupled to RISM to obtain the solvation structure and thermodynamics of oligomeric polyelectrolytes and drug-like compounds at a finite concentration in electrolyte solution, for which no convergence is obtained with other closures. For comparison, we calculate their solvation

  17. Macromolecule simulation and CH4 adsorption mechanism of coal vitrinite (United States)

    Yu, Song; Yan-ming, Zhu; Wu, Li


    The microscopic mechanism of interactions between CH4 and coal macromolecules is of significant practical and theoretical importance in CBM development and methane storage. Under periodic boundary conditions, the optimal energy configuration of coal vitrinite, which has a higher torsion degree and tighter arrangement, can be determined by the calculation of molecular mechanics (MM) and molecular dynamics (MD), and annealing kinetics simulation based on ultimate analysis, 13C NMR, FT IR and HRTEM. Macromolecular stabilization is primarily due to the van der Waals energy and covalent bond energy, mainly consisting of bond torsion energy and bond angle energy. Using the optimal configuration as the adsorbent, GCMC simulation of vitrinite adsorption of CH4 is conducted. A saturated state is reached after absorbing 17 CH4s per coal vitrinite molecule. CH4 is preferentially adsorbed on the edge, and inclined to gathering around the branched chains of the inner vitrinite sites. Finally, the adsorption parameters are calculated through first principle DFT. The adsorbability order is as follows: aromatic structure> heteroatom rings > oxygen functional groups. The adsorption energy order is as follows: Top < Bond < Center, Up < Down. The order of average RDF better reflects the adsorption ability and that of [-COOH] is lower than those of [sbnd Cdbnd O] and [Csbnd Osbnd C]. CH4 distributed in the distance of 0.99-16 Å to functional groups in the type of monolayer adsorption and the average distance order manifest as [sbnd Cdbnd O] (1.64 Å) < [Csbnd Osbnd C] (1.89 Å) < [sbnd COOH] (3.78 Å) < [-CH3] (4.11 Å) according to the average RDF curves. CH4 enriches around [sbnd Cdbnd O] and [Csbnd O-C] whereas is rather dispersed about [-COOH] and [CH3]. Simulation and experiment data are both in strong agreement with the Langmuir and D-A isothermal adsorption model and the D-A model fit better than Langmuir model. Preferential adsorption sites and orientations in vitrinite are

  18. Nonlinear cochlear mechanics. (United States)

    Zweig, George


    An earlier paper characterizing the linear mechanical response of the organ of Corti [J. Acoust. Soc. Am. 138, 1102-1121 (2015)] is extended to the nonlinear domain. Assuming the existence of nonlinear oscillators nonlocally coupled through the pressure they help create, the oscillator equations are derived and examined when the stimuli are modulated tones and clicks. The nonlinearities are constrained by the requirements of oscillator stability and the invariance of zero crossings in the click response to changes in click amplitude. The nonlinear oscillator equations for tones are solved in terms of the fluid pressure that drives them, and its time derivative, presumably a proxy for forces created by outer hair cells. The pressure equation is reduced to quadrature, the integrand depending on the oscillators' responses. The resulting nonlocally coupled nonlinear equations for the pressure, and oscillator amplitudes and phases, are solved numerically in terms of the fluid pressure at the stapes. Methods for determining the nonlinear damping directly from measurements are described. Once the oscillators have been characterized from their tone and click responses, the mechanical response of the cochlea to natural sounds may be computed numerically. Signal processing inspired by cochlear mechanics opens up a new area of nonlocal nonlinear time-frequency analysis.

  19. Surface characteristics and adhesion behavior of Escherichia coli O157:H7: role of extracellular macromolecules. (United States)

    Kim, Hyunjung N; Hong, Yongsuk; Lee, Ilkeun; Bradford, Scott A; Walker, Sharon L


    Experiments were conducted using enterohemorrhagic Escherichia coli O157:H7 cells to investigate the influence of extracellular macromolecules on cell surface properties and adhesion behavior to quartz sand. Partial removal of the extracellular macromolecules on cells by a proteolytic enzyme (proteinase K) was confirmed using Fourier transform infrared spectroscopy analyses. The proteinase K treated cells exhibited more negative electrophoretic mobility (EPM) at an ionic strength (IS) macromolecules resulted in polymeric layers outside the cell surface that were less electrophoretically soft. The more negative mobility for the treated cells was likely due to the combined effects of a change in the distribution of functional groups and an increase in the charges per unit volume after enzyme treatment and not just removal of extracellular macromolecules. The proteolytic digestion of extracellular macromolecules led to a significant difference in the cell adhesion to quartz sand. The adhesion behavior for treated cells was consistent with DLVO theory and increased with IS due to less negativity in the EPM. In contrast, the adhesion behavior of untreated cells was much more complex and exhibited a maximum at IS = 1 mM. The treated cells exhibited less adhesion than the untreated cells when the IS or = 10 mM, a sudden decrease in the removal efficiency was observed only for the untreated cells even through EPM values were similar for both treated and untreated cells. This result suggested that an additional non-DLVO type interaction, electrosteric repulsion, occurred at higher IS (> or =10 mM in this study) for the untreated cells due to the presence of extracellular macromolecules that hindered cell adhesion to the quartz surface. This finding provides important insight into the role of macromolecule-induced E. coli O157:H7 interactions in aquatic environments.

  20. Nonlinear optical processes at quadrupole polariton resonance in Cu2O as probed by a Z-scan technique (United States)

    Mani, S.; Jang, J. I.; Ketterson, J. B.


    Employing a modified Z-scan technique at 2 K, we monitor not only the fundamental (ω) but also the frequency-doubled (2ω) and tripled (3ω) Z-scan responses in Cu2O when the input laser frequency ω is tuned to the two-photon quadrupole polariton resonance. The Z-scan response at ω allows us to accurately estimate the absolute number of polaritons generated via two-photon absorption. A striking dip is observed near the 2ω Z-scan focus which basically arises from Auger-type recombination of polaritons. Under high excitation levels, the 3ω Z-scan shows strong third harmonic generation. Based on the nonlinear optical parameters determined, we estimate the experimental polariton density achievable and propose a direction for polariton-based Bose-Einstein condensation in Cu2O .

  1. Real-space post-processing correction of thermal drift and piezoelectric actuator nonlinearities in scanning tunneling microscope images

    CERN Document Server

    Yothers, Mitchell P; Bumm, Lloyd A


    We have developed a real-space method to correct distortion due to thermal drift and piezoelectric actuator nonlinearities on scanning tunneling microscope images using Matlab. The method uses the known structures typically present in high-resolution atomic and molecularly-resolved images as an internal standard. Each image feature (atom or molecule) is first identified in the image. The locations of each feature's nearest neighbors (NNs) are used to measure the local distortion at that location. The local distortion map across the image is simultaneously fit to our distortion model, which includes thermal drift in addition to piezoelectric actuator hysteresis and creep. The image coordinates of the features and image pixels are corrected using an inverse transform from the distortion model. We call this technique the thermal-drift, hysteresis, and creep transform (DHCT). Performing the correction in real space allows defects, domain boundaries, and step edges to be excluded with a spatial mask. Additional re...

  2. Cobalt-mediated radical coupling (CMRC): an unusual route to midchain-functionalized symmetrical macromolecules. (United States)

    Debuigne, Antoine; Poli, Rinaldo; De Winter, Julien; Laurent, Pascal; Gerbaux, Pascal; Dubois, Philippe; Wathelet, Jean-Paul; Jérôme, Christine; Detrembleur, Christophe


    Cobalt-mediated radical coupling (CMRC) is a straightforward approach to the synthesis of symmetrical macromolecules that relies on the addition of 1,3-diene compounds onto polymer precursors preformed by cobalt-mediated radical polymerization (CMRP). Mechanistic features that make this process so efficient for radical polymer coupling are reported here. The mechanism was established on the basis of NMR spectroscopy and MALDI-MS analyses of the coupling product and corroborated by DFT calculations. A key feature of CMRC is the preferential insertion of two diene units in the middle of the chain of the coupling product mainly according to a trans-1,4-addition pathway. The large tolerance of CMRC towards the diene structure is demonstrated and the impact of this new coupling method on macromolecular engineering is discussed, especially for midchain functionalization of polymers. It is worth noting that the interest in CMRC goes beyond the field of polymer chemistry, since it constitutes a novel carbon-carbon bond formation method that could be applied to small organic molecules.

  3. Role of macromolecules in the safety of use of body wash cosmetics. (United States)

    Bujak, Tomasz; Wasilewski, Tomasz; Nizioł-Łukaszewska, Zofia


    One of the most challenging problems related to the use of surfactants in body wash cosmetics is their potential to cause skin irritations. Surfactants can bind with proteins, remove lipids from the epidermal surface, contribute to the disorganization of liquid crystal structures in the intercellular lipids, and interact with living skin cells. These processes can lead to skin irritations and allergic reactions, and impair the epidermal barrier function. The present study is an attempt to assess the effect of polymers and hydrolysed proteins present in the formulations of model body wash cosmetics on product properties. Special attention was given to the safety of use of this product type. The study examined three macromolecules: polyvinylpyrrolidone (PVP), hydrolysed wheat protein (HWP) and polyvinylpyrrolidone/hydrolysed wheat protein crosspolymer (PVP/HWP). The addition of the substances under study was found to improve the foaming properties of body wash cosmetics, increase their stability during storage, and contribute significantly to an improvement in the safety of product use by reducing the irritant potential. The strongest ability to reduce the skin irritation potential was determined for the formula enriched with the PVP/HWP crosspolymer.

  4. New beamline dedicated to solution scattering from biological macromolecules at the ESRF

    Energy Technology Data Exchange (ETDEWEB)

    Pernot, P; Theveneau, P; Giraud, T; Fernandes, R Nogueira; Nurizzo, D; Spruce, D; Surr, J; McSweeney, S [ESRF, BP 220, Grenoble (France); Round, A; Felisaz, F; Foedinger, L; Gobbo, A; Huet, J; Villard, C; Cipriani, F, E-mail: rejma@esrf.f, E-mail: around@embl.f [EMBL Grenoble, BP 181, Grenoble (France)


    The new bio-SAXS beamline (ID14-3 at the ESRF, Grenoble, France) is dedicated exclusively to small-angle scattering experiments of biological macromolecules in solution and has been in user operation since November 2008. Originally a protein crystallography beamline, ID14-3 was refurbished, still as a part of the ESRF Structural Biology group, with the main aim to provide a facility with 'quick and easy' access to satisfy rapidly growing demands from crystallographers, biochemists and structural biologists. The beamline allows manual and automatic sample loading/unloading, data collection, processing (conversion of a 2D image to a normalized 1D X-ray scattering profile) and analysis. The users obtain on-line standard data concerning the size (radius of gyration, maximum dimension and volume) and molecular weight of samples which allow on-the fly ab-inito shape reconstruction in order to provide feedback enabling the data collection strategies to be optimized. Automation of sample loading is incorporated on the beamline using a device constructed in collaboration between the EMBL (Grenoble and Hamburg outstations) and the ESRF. Semi/automated data analysis is implemented following the model of the SAXS facility at X33, EMBL Hamburg. This paper describes the bio-SAXS beamline and set-up characteristics together with the examples of user data obtained.

  5. Ultrafast nonlinear optics

    CERN Document Server

    Leburn, Christopher; Reid, Derryck


    The field of ultrafast nonlinear optics is broad and multidisciplinary, and encompasses areas concerned with both the generation and measurement of ultrashort pulses of light, as well as those concerned with the applications of such pulses. Ultrashort pulses are extreme events – both in terms of their durations, and also the high peak powers which their short durations can facilitate. These extreme properties make them powerful experiment tools. On one hand, their ultrashort durations facilitate the probing and manipulation of matter on incredibly short timescales. On the other, their ultrashort durations can facilitate high peak powers which can drive highly nonlinear light-matter interaction processes. Ultrafast Nonlinear Optics covers a complete range of topics, both applied and fundamental in nature, within the area of ultrafast nonlinear optics. Chapters 1 to 4 are concerned with the generation and measurement of ultrashort pulses. Chapters 5 to 7 are concerned with fundamental applications of ultrasho...

  6. Nonlinear Reconstruction

    CERN Document Server

    Zhu, Hong-Ming; Pen, Ue-Li; Chen, Xuelei; Yu, Hao-Ran


    We present a direct approach to non-parametrically reconstruct the linear density field from an observed non-linear map. We solve for the unique displacement potential consistent with the non-linear density and positive definite coordinate transformation using a multigrid algorithm. We show that we recover the linear initial conditions up to $k\\sim 1\\ h/\\mathrm{Mpc}$ with minimal computational cost. This reconstruction approach generalizes the linear displacement theory to fully non-linear fields, potentially substantially expanding the BAO and RSD information content of dense large scale structure surveys, including for example SDSS main sample and 21cm intensity mapping.

  7. Nonlinear optics

    CERN Document Server

    Boyd, Robert W


    Nonlinear Optics is an advanced textbook for courses dealing with nonlinear optics, quantum electronics, laser physics, contemporary and quantum optics, and electrooptics. Its pedagogical emphasis is on fundamentals rather than particular, transitory applications. As a result, this textbook will have lasting appeal to a wide audience of electrical engineering, physics, and optics students, as well as those in related fields such as materials science and chemistry.Key Features* The origin of optical nonlinearities, including dependence on the polarization of light* A detailed treatment of the q

  8. Nanoscale amphiphilic macromolecules as lipoprotein inhibitors: the role of charge and architecture (United States)

    Wang, Jinzhong; Plourde, Nicole M; Iverson, Nicole; Moghe, Prabhas V; Uhrich, Kathryn E


    A series of novel amphiphilic macromolecules composed of alkyl chains as the hydrophobic block and poly(ethylene glycol) as the hydrophilic block were designed to inhibit highly oxidized low density lipoprotein (hoxLDL) uptake by synthesizing macromolecules with negatively charged moieties (ie, carboxylic acids) located in the two different blocks. The macromolecules have molecular weights around 5,500 g/mol, form micelles in aqueous solution with an average size of 20–35 nm, and display critical micelle concentration values as low as 10−7 M. Their charge densities and hydrodynamic size in physiological buffer solutions correlated with the hydrophobic/hydrophilic block location and quantity of the carboxylate groups. Generally, carboxylate groups located in the hydrophobic block destabilize micelle formation more than carboxylate groups in the hydrophilic block. Although all amphiphilic macromolecules inhibited unregulated uptake of hoxLDL by macrophages, inhibition efficiency was influenced by the quantity and location of the negatively charged-carboxylate on the macromolecules. Notably, negative charge is not the sole factor in reducing hoxLDL uptake. The combination of smaller size, micellar stability and charge density is critical for inhibiting hoxLDL uptake by macrophages. PMID:18203436

  9. Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production. (United States)

    Brudzynski, Katrina; Miotto, Danielle; Kim, Linda; Sjaarda, Calvin; Maldonado-Alvarez, Liset; Fukś, Henryk


    Little is known about the global structure of honey and the arrangement of its main macromolecules. We hypothesized that the conditions in ripened honeys resemble macromolecular crowding in the cell and affect the concentration, reactivity, and conformation of honey macromolecules. Combined results from UV spectroscopy, DLS and SEM showed that the concentration of macromolecules was a determining factor in honey structure. The UV spectral scans in 200-400 nm visualized and allowed quantification of UV-absorbing compounds in the following order: dark > medium > light honeys (p macromolecules promoted their self-assembly to micron-size superstructures, visible in SEM as two-phase system consisting of dense globules distributed in sugar solution. These particles showed increased conformational stability upon dilution. At the threshold concentration, the system underwent phase transition with concomitant fragmentation of large micron-size particles to nanoparticles in hierarchical order. Honey two-phase conformation was an essential requirement for antibacterial activity and hydrogen peroxide production. These activities disappeared beyond the phase transition point. The realization that active macromolecules of honey are arranged into compact, stable multicomponent assemblies with colloidal properties reframes our view on global structure of honey and emerges as a key property to be considered in investigating its biological activity.

  10. Cathepsin B degradable star-shaped peptidic macromolecules for delivery of 2-methoxyestradiol. (United States)

    Shankar, Ravi; Samykutty, Abhilash; Riggin, Corinne; Kannan, Sneha; Wenzel, Ursula; Kolhatkar, Rohit


    2-Methoxyestradiol (2ME), a natural metabolite of estradiol, has antiproliferative and antiangiogenic activity. However, its clinical success is limited due to poor water solubility and poor pharmacokinetic parameters suggesting the need for a delivery vehicle. In this study we evaluated cathepsin B degradable star-shaped peptidic macromolecules (SPMs) that can potentially be used to create higher generation and high molecular weight peptidic polymer as delivery vehicle of 2ME. Two peptidic macromolecules having positively charged amine (ASPM) or negatively charged carboxyl surface groups (CSPM) were synthesized and evaluated for their degradation in the presence of cathepsin B and stability in the presence of neutral or acidic buffer and serum. Both ASPM and CSPM degraded rapidly in the presence of cathepsin B. Both were stable in neutral and acidic buffer whereas only CSPM exhibited substantial stability in the presence of serum. Both macromolecules were nontoxic toward breast cancer cells whereas 2ME-containing macromolecules exhibited antiproliferative activity in the micromolar range. Overall, results from the current study indicate that tetrapeptide GFLG can be used to create star-shaped macromolecules that are degraded in the presence of cathepsin B and have the potential to be developed as delivery vehicles of 2ME.

  11. Locust bean gum in the development of sustained release mucoadhesive macromolecules of aceclofenac. (United States)

    Prajapati, Vipul D; Jani, Girish K; Moradiya, Naresh G; Randeria, Narayan P; Maheriya, Pankaj M; Nagar, Bhanu J


    The study shows the development and optimization of locust bean gum (LBG)-alginate mucoadhesive macromolecules containing aceclofenac through ionotropic-gelation using 3(2) factorial design. The effect of amount of LBG and sodium alginate on drug entrapment efficiency (%DEE), % mucoadhesion at 8h (M8) and % in vitro drug release at 10h (%Q10h) were optimized. The percentage yield, average size and DEE of macromolecules were found within the range of 93.19 to 96.65%, 1.328 ± 0.11 to 1.428 ± 0.13 μm, and 56.37 to 68.54%, respectively. The macromolecules were also characterized by SEM, FTIR and DSC. The in vitro drug release from these macromolecules (84.95 ± 2.02 to 95.33 ± 1.56% at 10h) exhibited sustained release (first-order) pattern with super case-II transport mechanism. The swelling and mucoadhesivity of these macromolecules were affected by pH of the medium. The design established the role of derived polynomial equations and plots in predicting the values of dependent variables for the preparation and optimization. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Filamentation with nonlinear Bessel vortices. (United States)

    Jukna, V; Milián, C; Xie, C; Itina, T; Dudley, J; Courvoisier, F; Couairon, A


    We present a new type of ring-shaped filaments featured by stationary nonlinear high-order Bessel solutions to the laser beam propagation equation. Two different regimes are identified by direct numerical simulations of the nonlinear propagation of axicon focused Gaussian beams carrying helicity in a Kerr medium with multiphoton absorption: the stable nonlinear propagation regime corresponds to a slow beam reshaping into one of the stationary nonlinear high-order Bessel solutions, called nonlinear Bessel vortices. The region of existence of nonlinear Bessel vortices is found semi-analytically. The influence of the Kerr nonlinearity and nonlinear losses on the beam shape is presented. Direct numerical simulations highlight the role of attractors played by nonlinear Bessel vortices in the stable propagation regime. Large input powers or small cone angles lead to the unstable propagation regime where nonlinear Bessel vortices break up into an helical multiple filament pattern or a more irregular structure. Nonlinear Bessel vortices are shown to be sufficiently intense to generate a ring-shaped filamentary ionized channel in the medium which is foreseen as opening the way to novel applications in laser material processing of transparent dielectrics.

  13. Nonlinear contact between pipeline's outer wall and slip-on buckle arrestor's inner wall during buckling process (United States)

    Ma, Weilin; Liu, Jiande; Dong, Sheng; Zhang, Xin; Ma, Xiaozhou


    In order to theoretically study the buckle propagation of subsea pipelines with slip-on buckle arrestors, a two-dimensional ring model was set up to represent the pipeline and a nonlinear spring model was adopted to simulate the contact between pipeline's inner walls and between pipeline's outer wall and slip-on buckle arrestor's inner wall during buckle propagation. In addition, some reverse springs are added to prevent the wall of left and right sides separating from the inner wall of slip-on buckle arrestors. Considering large deformation kinematics relations and the elastic-plastic constitutive relation of material, balance equations were established with the principle of virtual work. The variation of external pressure with respect to the cross-sectional area of pipelines was analyzed, and the lower bound of the crossover pressure of slip-on buckle arrestors was calculated based on Maxwell's energy balance method. By comparing the theoretical results with experiment and finite element numerical simulation, the theoretical method is proved to be correct and reliable.

  14. Nonlinear optimization

    CERN Document Server

    Ruszczynski, Andrzej


    Optimization is one of the most important areas of modern applied mathematics, with applications in fields from engineering and economics to finance, statistics, management science, and medicine. While many books have addressed its various aspects, Nonlinear Optimization is the first comprehensive treatment that will allow graduate students and researchers to understand its modern ideas, principles, and methods within a reasonable time, but without sacrificing mathematical precision. Andrzej Ruszczynski, a leading expert in the optimization of nonlinear stochastic systems, integrates t

  15. The effect of composition on diffusion of macromolecules in a crowded environment. (United States)

    Kondrat, Svyatoslav; Zimmermann, Olav; Wiechert, Wolfgang; von Lieres, Eric


    We study diffusion of macromolecules in a crowded cytoplasm-like environment, focusing on its dependence on composition and its crossover to the anomalous subdiffusion. The crossover and the diffusion itself depend on both the volume fraction and the relative concentration of macromolecules. In accordance with previous theoretical and experimental studies, diffusion slows down when the volume fraction increases. Contrary to expectations, however, the diffusion is also strongly dependent on the molecular composition. The crossover time decreases and diffusion slows down when the smaller macromolecules start to dominate. Interestingly, diffusion is faster in a cytoplasm-like (more polydisperse) system than it is in a two-component system, at comparable packing fractions, or even when the cytoplasm packing fraction is larger.

  16. Anisotropic diffusion of macromolecules in the contiguous nucleocytoplasmic fluid during eukaryotic cell division. (United States)

    Pawar, Nisha; Donth, Claudia; Weiss, Matthias


    Character and rapidity of protein diffusion in intracellular fluids are key determinants of the dynamics and steady state of a plethora of biochemical reactions. So far, an anomalous diffusion in cytoplasmic fluids with viscoelastic and even glassy characteristics has been reported in a variety of organisms on several length scales and timescales. Here, we show that the contiguous fluid of former cytoplasm and nucleoplasm features an anisotropically varying diffusion of macromolecules during eukaryotic cell division. In metaphase, diffusion in the contiguous nucleocytoplasmic fluid appears less anomalous along the spindle axis as compared to perpendicular directions. As a consequence, the long-time diffusion of macromolecules preferentially points along the spindle axis, leading to prolonged residence of macromolecules in the spindle region. Based on our experimental data, we suggest that anisotropic diffusion facilitates the encounter and interaction of spindle-associated proteins, e.g., during the formation of a dynamic spindle matrix. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Nonuniform elastic properties of macromolecules and effect of prestrain on their continuum nature. (United States)

    Aggarwal, Ankush; May, Eric R; Brooks, Charles L; Klug, William S


    Many experimental and theoretical methods have been developed to calculate the coarse-grained continuum elastic properties of macromolecules. However, all of those methods assume uniform elastic properties. Following the continuum mechanics framework, we present a systematic way of calculating the nonuniform effective elastic properties from atomic thermal fluctuations obtained from molecular dynamics simulation at any coarse-grained scale using a potential of the mean-force approach. We present the results for a mutant of Sesbania mosaic virus capsid, where we calculate the elastic moduli at different scales and observe an apparent problem with the chosen reference configuration in some cases. We present a possible explanation using an elastic network model, where inducing random prestrain results in a similar behavior. This phenomenon provides a novel insight into the continuum nature of macromolecules and defines the limits on details that the elasticity theory can capture. Further investigation into prestrains could elucidate important aspects of conformational dynamics of macromolecules.

  18. Scalable synthesis of sequence-defined, unimolecular macromolecules by Flow-IEG. (United States)

    Leibfarth, Frank A; Johnson, Jeremiah A; Jamison, Timothy F


    We report a semiautomated synthesis of sequence and architecturally defined, unimolecular macromolecules through a marriage of multistep flow synthesis and iterative exponential growth (Flow-IEG). The Flow-IEG system performs three reactions and an in-line purification in a total residence time of under 10 min, effectively doubling the molecular weight of an oligomeric species in an uninterrupted reaction sequence. Further iterations using the Flow-IEG system enable an exponential increase in molecular weight. Incorporating a variety of monomer structures and branching units provides control over polymer sequence and architecture. The synthesis of a uniform macromolecule with a molecular weight of 4,023 g/mol is demonstrated. The user-friendly nature, scalability, and modularity of Flow-IEG provide a general strategy for the automated synthesis of sequence-defined, unimolecular macromolecules. Flow-IEG is thus an enabling tool for theory validation, structure-property studies, and advanced applications in biotechnology and materials science.

  19. Improved microchip design and application for in situ transmission electron microscopy of macromolecules. (United States)

    Dukes, Madeline J; Thomas, Rebecca; Damiano, John; Klein, Kate L; Balasubramaniam, Sharavanan; Kayandan, Sanem; Riffle, Judy S; Davis, Richey M; McDonald, Sarah M; Kelly, Deborah F


    Understanding the fundamental properties of macromolecules has enhanced the development of emerging technologies used to improve biomedical research. Currently, there is a critical need for innovative platforms that can illuminate the function of biomedical reagents in a native environment. To address this need, we have developed an in situ approach to visualize the dynamic behavior of biomedically relevant macromolecules at the nanoscale. Newly designed silicon nitride devices containing integrated "microwells" were used to enclose active macromolecular specimens in liquid for transmission electron microscopy imaging purposes.We were able to successfully examine novel magnetic resonance imaging contrast reagents, micelle suspensions, liposome carrier vehicles, and transcribing viral assemblies. With each specimen tested, the integrated microwells adequately maintained macromolecules in discrete local environments while enabling thin liquid layers to be produced.

  20. Equilibrium partitioning of macromolecules in confining geometries: Improved universality with a new molecular size parameter

    DEFF Research Database (Denmark)

    Wang, Yanwei; Peters, Günther H.J.; Hansen, Flemming Yssing


    We present a new framework for the description of macromolecules subject to confining geometries. The two main ingredients are a new computational method and the definition of a new molecular size parameter. The computational method, hereafter referred to the confinement analysis from bulk...... structures (CABS), allows the computation of equilibrium partition coefficients as a function of confinement size solely based on a single sampling of the configuration space of a macromolecule in bulk. Superior in computational speed to previous computational methods, CABS is capable of handling slits...... parameter for characterization of spatial confinement effects on macromolecules. Results for the equilibrium partition coefficient in the weak confinement regime depend only on the ratio ofR-s to the confinement size regardless of molecular details....

  1. Extracellular matrix macromolecules: potential tools and targets in cancer gene therapy. (United States)

    Sainio, Annele; Järveläinen, Hannu


    Tumour cells create their own microenvironment where they closely interact with a variety of soluble and non-soluble molecules, different cells and numerous other components within the extracellular matrix (ECM). Interaction between tumour cells and the ECM is bidirectional leading to either progression or inhibition of tumourigenesis. Therefore, development of novel therapies targeted primarily to tumour microenvironment (TME) is highly rational. Here, we give a short overview of different macromolecules of the ECM and introduce mechanisms whereby they contribute to tumourigenesis within the TME. Furthermore, we present examples of individual ECM macromolecules as regulators of cell behaviour during tumourigenesis. Finally, we focus on novel strategies of using ECM macromolecules as tools or targets in cancer gene therapy in the future.

  2. Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development. (United States)

    van Lessen, Max; Shibata-Germanos, Shannon; van Impel, Andreas; Hawkins, Thomas A; Rihel, Jason; Schulte-Merker, Stefan


    The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc. BLECs endocytose macromolecules in a selective manner, which can be blocked by injection of mannose receptor ligands. This first report on brain lymphatic endothelial cells in a vertebrate embryo identifies cells with unique features, including the uptake of macromolecules at a single cell level. Future studies will address whether this represents an uptake mechanism that is conserved in mammals and how these cells affect functions of the embryonic and adult brain.

  3. Silicification and biosilicification: The role of macromolecules in bioinspired silica synthesis (United States)

    Patwardhan, Siddharth Vijay

    Diatoms, sponges and grasses are all known to produce ornate biogenic silica structures under ambient conditions. Some aspects of the molecular mechanism controlling biosilicification have recently been elucidated. The entrapment of the catalyzing/templating/scaffolding biomacromolecules enables them to be recovered by selective dissolution of biosilica. The proteins extracted from the diatom Cylindrotheca fusiformis (silaffins) and the sponge Tethya aurantia (silicateins) have been shown to precipitate silica from silica precursors in vitro. The identification of synthetic macromolecules that can act as catalysts/templates/scaffolds for silica formation gives exciting possibilities for bioinspired silica synthesis. Herein, the role of various synthetic (bio)macromolecules in silicification is studied. Attempts have also been made to understand the mechanism(s) governing (bio)macromolecule mediated (bio)silicification. Furthermore, the results and the understanding gained from various synthetic systems are used to demonstrate the potential of such bioinspired routes to develop new materials.

  4. A novel stress-accurate FE technology for highly non-linear analysis with incompressibility constraint. Application to the numerical simulation of the FSW process (United States)

    Chiumenti, M.; Cervera, M.; Agelet de Saracibar, C.; Dialami, N.


    In this work a novel finite element technology based on a three-field mixed formulation is presented. The Variational Multi Scale (VMS) method is used to circumvent the LBB stability condition allowing the use of linear piece-wise interpolations for displacement, stress and pressure fields, respectively. The result is an enhanced stress field approximation which enables for stress-accurate results in nonlinear computational mechanics. The use of an independent nodal variable for the pressure field allows for an adhoc treatment of the incompressibility constraint. This is a mandatory requirement due to the isochoric nature of the plastic strain in metal forming processes. The highly non-linear stress field typically encountered in the Friction Stir Welding (FSW) process is used as an example to show the performance of this new FE technology. The numerical simulation of the FSW process is tackled by means of an Arbitrary-Lagrangian-Eulerian (ALE) formulation. The computational domain is split into three different zones: the work.piece (defined by a rigid visco-plastic behaviour in the Eulerian framework), the pin (within the Lagrangian framework) and finally the stirzone (ALE formulation). A fully coupled thermo-mechanical analysis is introduced showing the heat fluxes generated by the plastic dissipation in the stir-zone (Sheppard rigid-viscoplastic constitutive model) as well as the frictional dissipation at the contact interface (Norton frictional contact model). Finally, tracers have been implemented to show the material flow around the pin allowing a better understanding of the welding mechanism. Numerical results are compared with experimental evidence.

  5. Applications of nonlinear fiber optics

    CERN Document Server

    Agrawal, Govind


    * The only book describing applications of nonlinear fiber optics * Two new chapters on the latest developments: highly nonlinear fibers and quantum applications* Coverage of biomedical applications* Problems provided at the end of each chapterThe development of new highly nonlinear fibers - referred to as microstructured fibers, holey fibers and photonic crystal fibers - is the next generation technology for all-optical signal processing and biomedical applications. This new edition has been thoroughly updated to incorporate these key technology developments.The bo

  6. 求解非线性化工过程鲁棒数据校正的新方法%A New Method to Solve Robust Data Reconciliation in Nonlinear Process

    Institute of Scientific and Technical Information of China (English)

    周凌柯; 苏宏业; 褚健


    Data reconciliation is an effective technique for providing accurate and consistent value for chemical process. However, the presence of gross errors can severely bias the reconciled results. Robust estimators can significantly reduce the effect of gross errors and yield less-biased results. In this article, a new method is proposed to solve the robust data reconciliation problem of nonlinear chemical process. By using several technologies including linearization method, penalty function, virtual observation equation, and equivalent weights method, the robust data reconciliation problem can be transformed into least squares estimator problem which leads to the convenience in computation. Simulation results in a nonlinear chemical process demonstrate the efficiency of the proposed method.

  7. Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows. (United States)

    Jönsson, Peter; Jönsson, Bengt


    It has previously been observed that an externally applied hydrodynamic shear flow above a fluid lipid bilayer can change the local concentration of macromolecules that are associated with the lipid bilayer. The external liquid flow results in a hydrodynamic force on molecules protruding from the lipid bilayer, causing them to move in the direction of the flow. However, there has been no quantitative study about the magnitude of these forces. We here use finite element simulations to investigate how the magnitude of the external hydrodynamic forces varies with the size and shape of the studied macromolecule. The simulations show that the hydrodynamic force is proportional to the effective hydrodynamic area of the studied molecule, Ahydro, multiplied by the mean hydrodynamic shear stress acting on the membrane surface, σhydro. The parameter Ahydro depends on the size and shape of the studied macromolecule above the lipid bilayer and scales with the cross-sectional area of the molecule. We also investigate how hydrodynamic shielding from other surrounding macromolecules decreases Ahydro when the surface coverage of the shielding macromolecules increases. Experiments where the protein streptavidin is anchored to a supported lipid bilayer on the floor of a microfluidic channel were finally performed at three different surface concentrations, Φ = 1%, 6%, and 10%, where the protein is being moved relative to the lipid bilayer by a liquid flow through the channel. From photobleaching measurements of fluorescently labeled streptavidin we found the experimental drift data to be within good accuracy of the simulated results, less than 12% difference, indicating the validity of the results obtained from the simulations. In addition to giving a deeper insight into how a liquid flow can affect membrane-associated molecules in a lipid bilayer, we also see an interesting potential of using hydrodynamic flow experiments together with the obtained results to study the size and

  8. Synthesis and Characterization of a Chondroitin Sulfate Based Hybrid Bio/Synthetic Biomimetic Aggrecan Macromolecule (United States)

    Sarkar, Sumona

    Lower back pain resulting from intervertebral disc degeneration is one of the leading musculoskeletal disorders confronting our health system. In order to mechanically stabilize the disc early in the degenerative cascade and prevent the need for spinal fusion surgeries, we have proposed the development of a hybrid-bio/synthetic biomimetic proteoglycan macromolecule for injection into the disc in the early stages of degeneration. The goal of this thesis was to incorporate natural chondroitin sulfate (CS) chains into bottle brush polymer synthesis strategies for the fabrication of CS-macromolecules which mimic the proteoglycan structure and function while resisting enzymatic degradation. Both the "grafting-to" and "grafting-through" techniques of bottle brush synthesis were explored. CS was immobilized via a terminal primary amine onto a model polymeric backbone (polyacrylic acid) for investigation of the "grafting-to" strategy and an epoxy-amine step-growth polymerization technique was utilized for the "grafting-through" synthesis of CS-macromolecules with polyethylene glycol backbone segments. Incorporation of a synthetic polymeric backbone at the terminal amine of CS was confirmed via biochemical assays, 1H-NMR and FTIR spectroscopy, and CS-macromolecule size was demonstrated to be higher than that of natural CS via gel permeation chromatography, transmission electron microscopy and viscosity measurements. Further analysis of CS-macromolecule functionality indicated maintenance of natural CS properties such as high fixed charge density, high osmotic potential and low cytotoxicity with nucleus pulposus cells. These studies are the first attempt at the incorporation of natural CS into biomimetic bottle brush structures. CS-macromolecules synthesized via the methods developed in these studies may be utilized in the treatment and prevention of debilitating back pain as well as act as mimetics for other proteoglycans implicated in cartilage, heart valve, and nervous

  9. Robust Nonlinear Neural Codes (United States)

    Yang, Qianli; Pitkow, Xaq


    Most interesting natural sensory stimuli are encoded in the brain in a form that can only be decoded nonlinearly. But despite being a core function of the brain, nonlinear population codes are rarely studied and poorly understood. Interestingly, the few existing models of nonlinear codes are inconsistent with known architectural features of the brain. In particular, these codes have information content that scales with the size of the cortical population, even if that violates the data processing inequality by exceeding the amount of information entering the sensory system. Here we provide a valid theory of nonlinear population codes by generalizing recent work on information-limiting correlations in linear population codes. Although these generalized, nonlinear information-limiting correlations bound the performance of any decoder, they also make decoding more robust to suboptimal computation, allowing many suboptimal decoders to achieve nearly the same efficiency as an optimal decoder. Although these correlations are extremely difficult to measure directly, particularly for nonlinear codes, we provide a simple, practical test by which one can use choice-related activity in small populations of neurons to determine whether decoding is suboptimal or optimal and limited by correlated noise. We conclude by describing an example computation in the vestibular system where this theory applies. QY and XP was supported by a grant from the McNair foundation.

  10. Nonlinear Multiantenna Detection Methods

    Directory of Open Access Journals (Sweden)

    Chen Sheng


    Full Text Available A nonlinear detection technique designed for multiple-antenna assisted receivers employed in space-division multiple-access systems is investigated. We derive the optimal solution of the nonlinear spatial-processing assisted receiver for binary phase shift keying signalling, which we refer to as the Bayesian detector. It is shown that this optimal Bayesian receiver significantly outperforms the standard linear beamforming assisted receiver in terms of a reduced bit error rate, at the expense of an increased complexity, while the achievable system capacity is substantially enhanced with the advent of employing nonlinear detection. Specifically, when the spatial separation expressed in terms of the angle of arrival between the desired and interfering signals is below a certain threshold, a linear beamformer would fail to separate them, while a nonlinear detection assisted receiver is still capable of performing adequately. The adaptive implementation of the optimal Bayesian detector can be realized using a radial basis function network. Two techniques are presented for constructing block-data-based adaptive nonlinear multiple-antenna assisted receivers. One of them is based on the relevance vector machine invoked for classification, while the other on the orthogonal forward selection procedure combined with the Fisher ratio class-separability measure. A recursive sample-by-sample adaptation procedure is also proposed for training nonlinear detectors based on an amalgam of enhanced -means clustering techniques and the recursive least squares algorithm.

  11. FOREWORD: International Topical Workshop on Plasma Physics: Coherent Processes in Nonlinear Media. Sponsored by the ICTP (Trieste) and the European Union (Brussels) (United States)

    Shukla, P. K.; Bingham, R.; Stenflo, L.; Dawson, J. M.


    Starting in 1989 we have created a forum at the International Centre for Theoretical Physics, Trieste, where scientists from different parts of the world can meet and exchange information in the frontier areas of physics. In the three previous meetings, we focused on large amplitude waves and fields in plasmas, the physics of dusty plasmas, and wave-particle interactions and energization in plasmas. In 1995, we came up with a fresh idea of organizing somewhat enlarged but still well focused research topics that are cross-disciplinary. Thus, the usual 'fourth-week activity' of the Plasma Physics College at the ICTP was replaced by an International Topical Workshop on Plasma Physics: Coherent Processes in Nonlinear Media, which was held at the ICTP during the period 16-20 October, 1995. This provided us an opportunity to draw eminent speakers from many closely related fields such as plasma physics, fluid dynamics, nonlinear optics, and astrophysics. The Workshop was attended by 82 delegates from 34 countries, and the participation from the industrial and the developing countries was about half each. The programme included 4 review and 29 topical invited lectures. In addition, about 30 contributed papers were presented as posters in two sessions. The latter were created in order to give opportunities to younger physicists for displaying the results of their recent work and to obtain constructive comments from the other participants. During the five days at the ICTP, we focused on almost all the various aspects of nonlinear phenomena that are common in different branches of science. The review and topical lectures as well as the posters dealt with the most recent advances in coherent nonlinear processes in space and astrophysical plasmas, in fluids and optics, in low temperature dusty plasmas, as well as in laser produced and magnetically confined laboratory plasmas. The focus was on the physics of various types of waves and their generation mechanisms, the development

  12. Recent Issues on Nonlinear Effects in Optical Fibers

    Institute of Scientific and Technical Information of China (English)

    Takashi; Inoue; Osamu; Aso; Shu; Namiki


    This talk will discuss the types of optical signal degradation due to fiber nonlinearity and review recently invented fibers for suppressing the effects. It also introduces efficiency of highly nonlinear fibers and their applications to nonlinear signal processing.

  13. "Bio"-macromolecules: polymer-protein conjugates as emerging scaffolds for therapeutics. (United States)

    Borchmann, Dorothee E; Carberry, Tom P; Weck, Marcus


    Polymer-protein conjugates are biohybrid macromolecules derived from covalently connecting synthetic polymers with polypeptides. The resulting materials combine the properties of both worlds: chemists can engineer polymers to stabilize proteins, to add functionality, or to enhance activity; whereas biochemists can exploit the specificity and complexity that Nature has bestowed upon its macromolecules. This has led to a wealth of applications, particularly within the realm of biomedicine. Polymer-protein conjugation has expanded to include scaffolds for drug delivery, tissue engineering, and microbial inhibitors. This feature article reflects upon recent developments in the field and discusses the applications of these hybrids from a biomaterials standpoint.

  14. Single molecule optical measurements of orientation and rotations of biological macromolecules (United States)

    Shroder, Deborah Y.; Lippert, Lisa G.; Goldman, Yale E.


    Subdomains of macromolecules often undergo large orientation changes during their catalytic cycles that are essential for their activity. Tracking these rearrangements in real time opens a powerful window into the link between protein structure and functional output. Site-specific labeling of individual molecules with polarized optical probes and measurement of their spatial orientation can give insight into the crucial conformational changes, dynamics, and fluctuations of macromolecules. Here we describe the range of single molecule optical technologies that can extract orientation information from these probes, review the relevant types of probes and labeling techniques, and highlight the advantages and disadvantages of these technologies for addressing specific inquiries.

  15. Method for selective immobilization of macromolecules on self assembled monolayer surfaces (United States)

    Laskin, Julia [Richland, WA; Wang, Peng [Billerica, MA


    Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.

  16. Click chemistry approach to functionalize two-dimensional macromolecules of graphene oxide nanosheets

    Institute of Scientific and Technical Information of China (English)

    Liang Kou; Hongkun He; Chao Gao


    A facile “click chemistry” approach to functionalize 2D macromolecules of graphene oxide nanosheets with poly (ethylene glycol) of different molecular weights, polystyrene, palmitic acid and various amino acids was presented. FTIR, TGA, Raman spectroscopy, XPS, XRD, TEM, AFM and SEM were utilized to characterize the products. High degree of functionalization was achieved on the flat surfaces of graphene oxide, affording polymer-grafted 2D brushes and amino acids-immobilized nanosheets, which show improved solubility in organic solvents. The click chemistry strategy reported herein provides a facile and general method for functionalization of graphene oxide with macromolecules and desired biomolecules.

  17. A new bead-spring model for simulation of semi-flexible macromolecules (United States)

    Saadat, Amir; Khomami, Bamin


    A bead-spring model for semi-flexible macromolecules is developed to overcome the deficiencies of the current coarse-grained bead-spring models. Specifically, model improvements are achieved through incorporation of a bending potential. The new model is designed to accurately describe the correlation along the backbone of the chain, segmental length, and force-extension behavior of the macromolecule even at the limit of 1 Kuhn step per spring. The relaxation time of different Rouse modes is used to demonstrate the capabilities of the new model in predicting chain dynamics.

  18. Non-linear effects of initial melt temperatures on microstructures and mechanical properties during quenching process of liquid Cu46Zr54 alloy (United States)

    Mo, Yun-Fei; Liu, Rang-Su; Tian, Ze-An; Liang, Yong-Chao; Zhang, Hai-Tao; Hou, Zhao-Yang; Liu, Hai-Rong; Zhang, Ai-long; Zhou, Li-Li; Peng, Ping; Xie, Zhong


    A MD simulation of liquid Cu46Zr54 alloys has been performed for understanding the effects of initial melt temperatures on the microstructural evolution and mechanical properties during quenching process. By using several microstructural analyzing methods, it is found that the icosahedral and defective icosahedral clusters play a key role in the microstructure transition. All the final solidification structures obtained at different initial melt temperatures are of amorphous structures, and their structural and mechanical properties are non-linearly related to the initial melt temperatures, and fluctuated in a certain range. Especially, there exists a best initial melt temperature, from which the glass configuration possesses the highest packing density, the optimal elastic constants, and the smaller extent of structural softening under deforming.

  19. On the Nonlinear Response of Lower Stratospheric Ozone to External Forces-The Inclusion of BrOx and Radiation Processes

    Institute of Scientific and Technical Information of China (English)

    WANG Geli; YAN Jianjun; YANG Peicai


    In this paper the bromine family and radiative effects are considered in an updated box model under the framework of ozone temperature feedback,in order to further analyze the possible behavior of atmospheric ozone in the lower mid-latitude stratosphere.Results show that this updated photochemical system can present several different solutions,within a certain domain of parameters,with fixed-point and periodic states appearing in turn.The temperature feedback effect introduced in this box model has not changed the topology of the ozone system.This result presents nonlinear characteristics of the ozone system,and possible trends in the stratospheric atmosphere between complex chemistry and radiation processes.

  20. Chemically induced alterations in the characteristics of fouling-causing bio-macromolecules - Implications for the chemical cleaning of fouled membranes. (United States)

    Zhou, Zhongbo; He, Xiang; Zhou, Minghao; Meng, Fangang


    Chemical cleaning is an essential process for the permeability recovery of fouled membranes, which is highly related to the interactions between chemicals and bio-macromolecules in fouling layers. In this study, three bio-macromolecules (i.e., effluent biopolymers (i.e., 0.45 μm-100 kDa) from a full-scale municipal wastewater treatment plant, bovine serum albumin (BSA) and dextran) were exposed to different chemicals (i.e., NaClO, H2O2, NaOH, and HCl) with varied concentrations to understand the changes in their properties and functional groups. The results showed that exposure to oxidants and alkali decreased the consistency index of all bio-macromolecules. With an increased oxidant dose, the molecular sizes of effluent biopolymers and dextran continuously reduced because of the oxidative cleavage of the long molecule chains. However, the molecular size of BSA sharply increased after being treated with oxidants and alkali, likely due to the cross-linkage of protein molecules. Three-dimensional fluorescence excitation-emission matrix (3D-EEM) spectra showed that the aromatic protein-like and humic substances in the effluent biopolymers were destructed readily during the treatments of oxidants and alkali. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) analyse further confirmed that exposures to NaClO, H2O2 and NaOH led to the destruction of protein structures (i.e., amide I, II and III), the increase of carbonyl and carboxyl groups, and the decrease of fatty acids/lipids, all of which could make the bio-macromolecules more hydrophilic. Most importantly, the bio-macromolecules exposed to chemicals had better filterability, and their permeability through membranes also significantly increased, which could be explained well by the above analysis. The chemical cleaning mechanisms of fouled membranes are understood in depth in this study, and all of the results shed light on the implementation of on-line chemical enhanced backwashing in