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Sample records for non-segmental vitiligo patients

  1. The presence of non-segmental vitiligo modifies intracellular cytokine subsets in patients with chronic lymphocytic thyroiditis.

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    Santaguida, M G; Del Duca, S C; Virili, C; Gargano, L; Centanni, M

    2010-01-01

    Chronic lymphocytic thyroiditis and vitiligo often occur in association and seem to be characterized by a prevalent Th1-driven autoimmune process. The aim of this study is to analyze selected intracellular Τh1 and Th2 cytokines in patients with Hashimoto?s thyroiditis when associated with non-segmental vitiligo. We analyzed intracellular interleukin-2, interferon-gamma (Τh1) and interleukin-4 (Th2), in peripheral blood lymphocytes of 23 patients with isolated Hashimoto?s thyroiditis (group A) and of 11 patients with Hashimoto?s thyroiditis associated with non-segmental vitiligo (group B). Peripheral blood lymphocytes were stimulated and incubated with specific monoclonal antibodies. Intracellular cytokines were assayed by flow cytometric analysis. Interleukin-2 and interferon-gamma positive cells were increased in almost all patients but the median values were similar in patients with isolated Hashimoto?s thyroiditis and in those with concurrent vitiligo. In contrast, the number of patients with increased interleukin-4 positive cells was higher in patients with thyroiditis and vitiligo (9/11) than in those with isolated thyroiditis (2/23; plymphocytes characterize Hashimoto?s thyroiditis when associated with non-segmental vitiligo, suggesting a modified balance from highly prevalent Th1 to mixed Th1/Th2 subset.

  2. Differential expression analysis of miRNA in peripheral blood mononuclear cells of patients with non-segmental vitiligo.

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    Wang, Yi; Wang, Keyu; Liang, Jianhua; Yang, Hong; Dang, Ningning; Yang, Xi; Kong, Yi

    2015-02-01

    Vitiligo is a common depigmentary skin disease that may follow a pattern of multifactorial inheritance. The essential factors of its immunopathogenesis is thought to be the selective destruction of melanocytes. As a new class of microregulators of gene expression, miRNA have been reported to play vital roles in autoimmune diseases, metabolic diseases and cancer. This study sought to characterize the different miRNA expression pattern in the peripheral blood mononuclear cells (PBMC) of patients with non-segmental vitiligo (NSV) and healthy individuals and to examine their direct responses to thymosin α1 (Tα1) treatment. The miRNA expression profile in the PBMC of patients with NSV was analyzed using Exiqon's miRCURY LNA microRNA Array. The differentially expressed miRNA were validated by real-time quantitative polymerase chain reaction. We found that the expression levels of miR-224-3p and miR-4712-3p were upregulated, and miR-3940-5p was downregulated in the PBMC. The common clinical immune modulator Tα1 changed the miRNA expression profile. Our analysis showed that differentially expressed miRNA were associated with the mechanism of immune imbalance of vitiligo and that Tα1 could play an important role in changing the expression of these miRNA in the PBMC of patients with NSV. This study provided further evidence that miRNA may serve as novel drug targets for vitiligo therapeutic evaluation.

  3. Fractional CO2 lasers contribute to the treatment of stable non-segmental vitiligo.

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    Yuan, Jinping; Chen, Hongqiang; Yan, Ru; Cui, Shaoshan; Li, Yuan-Hong; Wu, Yan; Gao, Xing-Hua; Chen, Hong-Duo

    2016-12-01

    Stable non-segmental vitiligo is often resistant to conventional therapies. The purpose of this study was to investigate the effect of three types of fractional lasers in the treatment of stable non-segmental vitiligo. Twenty patients were enrolled in the study. The vitiligo lesions of each patient were divided into four treatment parts, and all parts were treated with narrowband ultraviolet-B (NB-UVB). Three of the four parts were respectively treated with three types of fractional lasers (two ablative 10,600-nm CO2 lasers and one non-ablative 1,565-nm laser), followed by topical betamethasone solution application. The treatment period lasted six months. Efficacy and satisfaction were respectively assessed by dermatologists and patients. The ablative CO2 lasers, in combination with topical betamethasone solution and NB-UVB, achieved marked to excellent improvement on white patches assessed by dermatologists. Patients showed high satisfaction scores for the treatments. The non-ablative 1,565-nm fractional laser did not provide any further benefit in the treatment of vitiligo. No severe adverse events developed for any of the treatments. The treatment protocol with ablative CO2 lasers, in combination with topical betamethasone solution and NB-UVB, was suitable for stable non-segmental vitiligo. For vitiligo, the ablative fractional CO2 laser is more effective than the non-ablative fractional laser.

  4. Effect of combination of fractional CO2 laser and narrow-band ultraviolet B versus narrow-band ultraviolet B in the treatment of non-segmental vitiligo.

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    El-Zawahry, Mohamed Bakr; Zaki, Naglaa Sameh; Wissa, Marian Youssry; Saleh, Marwah Adly

    2017-07-26

    The present study was designed to evaluate the effect of combining fractional CO2 laser with narrow-band ultraviolet B (NB-UVB) versus NB-UVB in the treatment of non-segmental vitiligo. The study included 20 patients with non-segmental stable vitiligo. They were divided into two groups. Group I received a single session of fractional CO2 laser therapy on the right side of the body followed by NB-UVB phototherapy twice per week for 8 weeks. Group II received a second session of fractional CO2 laser therapy after 4 weeks from starting treatment with NB-UVB. The vitiligo lesions were assessed before treatment and after 8 weeks of treatment by VASI. At the end of the study period, the vitiligo area score index (VASI) in group I decreased insignificantly on both the right (-2.6%) and left (-16.4%) sides. In group II, VASI increased insignificantly on the right (+14.4%) and left (+2.5%) sides. Using Adobe Photoshop CS6 extended program to measure the area of vitiligo lesions, group I showed a decrease of -1.02 and -6.12% in the mean area percentage change of vitiligo lesions on the right and left sides, respectively. In group II the change was +9.84 and +9.13% on the right and left sides, respectively. In conclusion, combining fractional CO2 laser with NB-UVB for the treatment of non-segmental vitiligo did not show any significant advantage over treatment with NB-UVB alone. Further study of this combination for longer durations in the treatment of vitiligo is recommended.

  5. Fractional Er:YAG laser assisting topical betamethasone solution in combination with NB-UVB for resistant non-segmental vitiligo.

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    Yan, Ru; Yuan, Jinping; Chen, Hongqiang; Li, Yuan-Hong; Wu, Yan; Gao, Xing-Hua; Chen, Hong-Duo

    2017-07-14

    Resistant non-segmental vitiligo is difficult to be treated. Ablative erbium-YAG (Er:YAG) laser has been used in the treatment of vitiligo, but the ablation of entire epidermis frustrated the compliance of patients. The purpose of this study is to investigate the effects of fractional Er:YAG laser followed by topical betamethasone and narrow band ultraviolet B (NB-UVB) therapy in the treatment of resistant non-segmental vitiligo. The vitiligo lesions of each enrolled patient were divided into four treatment parts, which were all irradiated with NB-UVB. Three parts were, respectively, treated with low, medium, or high energy of Er:YAG laser, followed by topical betamethasone solution application. A control part was spared with laser treatment and topical betamethasone. The treatment period lasted 6 months. The efficacy was assessed by two blinded dermatologists. Treatment protocol with high energy of 1800 mJ/P of fractional Er:YAG laser followed by topical betamethasone solution and in combination with NB-UVB made 60% patients achieve marked to excellent improvement in white patches. The protocol with medium energy of 1200 mJ/P of laser assisted approximate 36% patients achieve such improvement. The two protocols, respectively, showed better efficacies than NB-UVB only protocol. However, fractional Er:YAG laser at low energy of 600 mJ/P did not provide such contributions to the treatment of vitiligo. The fractional Er:YAG laser in combination with topical betamethasone solution and NB-UVB was suitable for resistant non-segmental vitiligo. The energy of laser was preferred to be set at relatively high level.

  6. Clinical analysis and treatment of 1 025 patients with segmental and non-segmental vitiligo:a preliminary survey%节段型与非节段型白癜风临床特征比较及治疗现状初步调查

    Institute of Scientific and Technical Information of China (English)

    叶莹; 周莲宝; 项蕾红

    2013-01-01

    目的:了解白癜风不同临床类型的相关特征和常用治疗方法的临床应用情况.方法:采用问卷调查方法,收集临床资料,输入EPIDATA 3.1数据库,应用STATA软件对数据进行统计学分析.结果:调查的1 025例白癜风患者中,男女比例为0.83:1,临床类型中节段型占19.0%,非节段型占81.0%;局限型占总人数的18.3%,散发型占56.4%,肢端型占5.3%,而泛发型只占1%.平均发病年龄(23.2±12.5)岁,主要集中在11~20岁(40.2%),疾病平均病程为38个月(中位数),复发率为6.7%.同形反应、伴有晕痣各占16.9%和7.8%,且非节段型患者发生的概率皆高于节段型患者.通过比较各种治疗方法,光疗及自体表皮移植的治疗有效率较高,分别为80.3%和92.7%,但临床应用率较低,仅为16.6%和20.5%.结论:白癜风病程长,复发率低,非节段型患者出现同形反应、晕痣的比率高于节段型.临床上应大力推广光疗,对于稳定期的患者,可行自体表皮移植治疗.%Objective: To study the clinical features and treatment of vitiligo through a large-population epidemiological survey. Methods: A questionnaire survey was carried out to collect clinical data from 1 025 cases of vitiligo. EpiData and STATA were utilized for statistical analysis. Results: In 1 025 patients with vitiligo, the ratio of male to female was 0.83 to 1. The mean age at onset was (23.2±12.5) years, mainly concentrated in the 11 to 20 years (40.2%). The mean duration of disease was 38 months. The most common type was non-segmental vitiligo (81%), while segmental type accounted for 19% of the 1 025 patients. Focal, generalized, acral, and universal type accounted for 18.3%, 56.4%, 5.3% and 1%, respectively. The relapse rate was 6.7%. The incidences of Koebner's phenomenon and halo nevus in the vitiliginous areas were 16.9% and 7.8%. The probabilities of these clinical features in the patients of non-segmental were both higher than those of the patients of segmental vi

  7. Audiological Manifestations in Vitiligo Patients

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    Parvane Mahdi

    2012-01-01

    Full Text Available Introduction: The hallmark of vitiligo is the disappearance of melanocytes from the skin due to an as yet unidentified mechanism. The presence of melanocytes in the auditory apparatus suggests that this system could possibly be affected by vitiligo, which targets the melanocytes of the whole body and not just the skin.The purpose of this study was to assess the incidence of auditory alterations in patients with vitiligo Materials and Methods: A total of 21 patients diagnosed with vitiligo were enrolled in this study. A group of 20 healthy subjects served as a control group. Pure tone audiometry (PTA and measurements of auditory brain stem responses (ABR were carried out in all participants. Results: High frequency sensory neural hearing loss was detected in 8 patients (38.09%. Analysis of ABR revealed that 10 patients (47.61% had an abnormal increase in the latency of Wave III and 6 (28.57% had an abnormal prolongation of the inter peak latency between Wave I and III. There was no correlation between age, duration of disease, and any of the recorded parameters. Conclusion: This study highlights the involvement of the auditory system in patients with vitiligo, suggesting that vitiligo is a systemic disease rather than a purely cutaneous problem.

  8. Increased circulating Th17 cells and elevated serum levels of TGF-beta and IL-21 are correlated with human non-segmental vitiligo development.

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    Zhou, Li; Shi, Yu-Ling; Li, Kai; Hamzavi, Iltefat; Gao, Tian-Wen; Huggins, Richard H; Lim, Henry W; Mi, Qing-Sheng

    2015-05-01

    Although non-segmental vitiligo (NSV) results from the autoimmune destruction of melanocytes, the detailed immune mechanisms have not yet been fully elucidated. Th17 cells have been identified to be implicated in human autoimmune diseases. In this study, the frequencies of peripheral blood Th17 cells and serum levels of IL-17A and Th17 cell-related cytokines were examined in 45 patients with active NSV compared to 45 race-, gender-, and age-matched healthy controls. Our results showed increased circulating Th17 cell frequencies and elevated serum IL-17A, TGF-β1, and IL-21 levels in patients with NSV. Meanwhile, the increased Th17 cell frequencies are positively correlated with serum TGF-β1 level, and the body surface area of lesions is positively correlated with elevated TGF-β1 and IL-21 levels and Th17 cell frequencies. Furthermore, positive correlation was identified between Th17 and Th1 cell frequencies in patients with NSV. These results further indicate the potential involvement of Th17 cells and the collaborative contribution of Th17 and Th1 in NSV development, and suggest that the elevated serum TGF-β1 and IL-21 levels could contribute to enhanced Th17 cell differentiation in NSV.

  9. Nail abnormalities in patients with vitiligo

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    Topal, Ilteris Oguz; Gungor, Sule; Kocaturk, Ozgur Emek; Duman, Hatice; Durmuscan, Mustafa

    2016-01-01

    Abstract: Background: Vitiligo is an acquired pigmentary skin disorder affecting 0.1-4% of the general population. The nails may be affected in patients with an autoimmune disease such as psoriasis, and in those with alopecia areata. It has been suggested that nail abnormalities should be apparent in vitiligo patients. Objective: We sought to document the frequency and clinical presentation of nail abnormalities in vitiligo patients compared to healthy volunteers. We also examined the corre...

  10. 不同类型白癜风基因谱表达差异的初步研究%Preliminary study of differential gene expression profiling between segmental vitiligo and non-segmental vitiligo

    Institute of Scientific and Technical Information of China (English)

    聂慧琼; 王平; 张小燕; 侯秀丽; 许文; 邵琼琰

    2016-01-01

    目的:探讨白癜风临床类型与基因表达差异的相关性。方法分别选择节段型白癜风( SV)、非节段型白癜风( NSV)和健康对照( HI)各4例,应用Phalanx人全基因组表达谱芯片检测外周血淋巴细胞基因谱表达差异,并通过RT-PCR进行验证。结果与HI相比,SV有239个上调基因和175个下调基因,主要涉及适应性免疫反应、细胞因子受体相互作用、趋化因子信号通路,NSV有88个上调基因和560个下调基因,涉及先天性免疫、细胞自噬与凋亡、黑素细胞生物学、泛素介导的蛋白裂解与酪氨酸酶代谢。 SV和NSV共同基因表达差异包括60个上调基因和60个下调基因;相对于SV,NSV表达223个上调基因和129个下调基因,主要包括嘌呤、嘧啶、鞘脂类代谢。结论 SV有不同于NSV的遗传背景与发病机制。%Objective To investigate the correlation between clinical pattern and differential gene expression in pa-tients with vitiligo. Methods Peripheral blood lymphocytes obtained from four cases of segmental vitiligo ( SV) , non-segmental vitiligo ( NSV ) and healthy individual ( HI ) respectively. Whole genome expression microarrays were used to assay the gene expression profiles between SV, NSV and HI. Quantitative PCR assay was used to vali-date the gene expression of array. Results Compared to HI, 239 over-expressed and 175 down-expressed genes were detected in SV, which were mainly involved in the adaptive immune response, cytokine-cytokine receptor in-teraction and chemokine signaling. In NSV, 88 over-expressed and 560 down-expressed genes were found and were mainly involved in the innate immune, autophagy, apoptosis, melanocyte biology, ubiquitin mediated proteolysis and tyrosine metabolism, which were different from SV. Sixty over-expressed and sixty down-expressed genes shared similar tendency in SV and NSV. Compared to SV, 223 over-regulated and 129 down-regulated genes were found in NSV, which were mainly

  11. Nail abnormalities in patients with vitiligo*

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    Topal, Ilteris Oguz; Gungor, Sule; Kocaturk, Ozgur Emek; Duman, Hatice; Durmuscan, Mustafa

    2016-01-01

    Background Vitiligo is an acquired pigmentary skin disorder affecting 0.1-4% of the general population. The nails may be affected in patients with an autoimmune disease such as psoriasis, and in those with alopecia areata. It has been suggested that nail abnormalities should be apparent in vitiligo patients. Objective We sought to document the frequency and clinical presentation of nail abnormalities in vitiligo patients compared to healthy volunteers. We also examined the correlations between nail abnormalities and various clinical parameters. Methods This study included 100 vitiligo patients and 100 healthy subjects. Full medical histories were collected from the subjects, who underwent thorough general and nail examinations. All nail changes were noted. In the event of clinical suspicion of a fungal infection, additional mycological investigations were performed. Results Nail abnormalities were more prevalent in the patients (78%) than in the controls (55%) (p=0.001). Longitudinal ridging was the most common finding (42%), followed by (in descending order): leukonychia, an absent lunula, onycholysis, nail bed pallor, onychomycosis, splinter hemorrhage and nail plate thinning. The frequency of longitudinal ridging was significantly higher in patients than in controls (p<0.001). Conclusions Nail abnormalities were more prevalent in vitiligo patients than in controls. Systematic examination of the nails in such patients is useful because nail abnormalities are frequent. However, the causes of such abnormalities require further study. Longitudinal ridging and leukonychia were the most common abnormalities observed in this study. PMID:27579738

  12. Generalized Vitiligo Associated Autoimmune Diseases in Japanese Patients Their Families

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    Tomohiko Narita

    2011-01-01

    Conclusions: Among Japanese vitiligo patients, there is a subgroup with strong evidence of genetically determined susceptibility to not only vitiligo, but also to autoimmune thyroid disease and other autoimmune disorders.

  13. The Vitiligo Impact Patient Scale (VIPs): Development and Validation of a Vitiligo Burden Assessment Tool.

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    Salzes, Camille; Abadie, Sophie; Seneschal, Julien; Whitton, Maxine; Meurant, Jean-Marie; Jouary, Thomas; Ballanger, Fabienne; Boralevi, Franck; Taieb, Alain; Taieb, Charles; Ezzedine, Khaled

    2016-01-01

    Vitiligo has a major impact on health-related quality of life. Although a few vitiligo-specific quality of life instruments exist, there is no specific vitiligo burden tool. We developed and validated a specific vitiligo burden tool according to skin phototype. In total, 301 patients completed 35 items of the Vitiligo Impact Patient scale, of whom 235 were of skin phototype I to III and 66 of phototype IV to VI. The dimensionality of the items was evaluated using factor analyses, with results suggesting three factors in fair- and dark-skinned patients ("Psychological effects on daily life," "Relationships and Sexuality," and "Economic Constraints, Care & Management of Disease"). Unidimensionality was confirmed by higher order factor analysis. Cronbach's α were high-and intradimensional coherences all demonstrated good reliability (α > 0.8). The final instrument consists of 29 items (19 items common to all patients, 3 specific to fair skin, and 7 to dark skin). The test-retest reliability demonstrated very good reproducibility. The intraclass correlation of each dimension was greater than 0.90 for each population. External validity was confirmed by the correlation coefficients and Bland and Altman plots of the Vitiligo Impact Patient scale-Fair Skin and Vitiligo Impact Patient scale-Dark Skin versus the Short-Form-12, PVC Metra, Body Image States Scale, and Daily Life Quality Index assessment tools.

  14. Evaluation of the serum zinc level in patients with vitiligo

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    Majid Rostami Mogaddam

    2017-04-01

    Full Text Available Introduction : Vitiligo is an acquired, idiopathic disorder characterized by circumscribed depigmented macules and patches, which affects approximately 0.1–2% of the general population worldwide. Zinc is an essential trace element that is necessary for growth and development at all stages of life. Some studies have reported an association between serum zinc levels and vitiligo. Aim : To measure the serum zinc level in patients with vitiligo compared to healthy subjects. Material and methods : One hundred patients with vitiligo and 100 healthy controls were referred to our clinic. The two groups were matched for age and sex. Atomic absorption spectrophotometry was used to measure serum zinc levels. The statistical analysis was performed using SPSS software. Results : The mean serum level of zinc in vitiligo patients and controls was 80.11 ±17.10 µg/dl and 96.10 ±16.16 µg/dl, respectively. The serum zinc level in patients with vitiligo was significantly lower than in healthy controls (p = 0.0001. Conclusions : The results of our study revealed a significant association between vitiligo and serum zinc levels. A relative decrease in the serum zinc level in vitiligo patients can highlight the role of zinc in the pathogenesis of vitiligo, and large-scale studies need to be conducted to confirm these findings and assess the effect of oral zinc supplements in patients with low zinc levels.

  15. Study on the Oxidant and Antioxidant Status in Vitiligo Patients

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    Fatma Hassan Shabaka*, Sawsan Khalifa EL- Sayed*,

    2007-09-01

    Full Text Available Backgrounds: The aetiology of vitiligo is still unknown. Several hypotheses have been proposed to explain vitiligo: genetic neural, immunological, self destructive, convergence hypothesis and oxidative stress hypothesis The current study is concerned with the oxidative stress hypothesis and how oxidants and antioxidants affect the pathogenesis of vitiligo. So, our aim is to determine the role of malondialdehyde and glutathione in the pathogenesis of vitiligo. The amount of malondialdehyde (oxidant and glutathione (antioxidant were measured in serum and in skin tissue in 30 vitiligo cases and 20 healthy controls Results: The study showed significant changes between patients and controls in glutathione level in blood and tissue samples. Also there were significant changes between patients and controls in malondialdehyde in blood and in tissue samples favoring that glutathione and malondialdehyde play a role in the pathogenesis of vitiligo.

  16. Diphenylcyclopropenone-Induced Vitiligo in a Patient with Alopecia Universalis

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    Hassan Riad

    2013-08-01

    Full Text Available Alopecia areata and vitiligo are autoimmune diseases, both associated with multiple autoimmune comorbidities. Many studies show colocalization of these diseases at the same anatomical site. Here, we have a case where both disorders were reported to present in the same patient. Diphenylcyclopropenone (diphencyprone, DCP is used in the treatment of alopecia areata and may induce vitiligo in some patients. We report on one case of vitiligo that was induced by DCP during therapy for alopecia universalis. Alopecia areata and vitiligo share many susceptibility genes. Follicular melanocyte destruction may represent the link between the two diseases.

  17. Assessment of auditory and vestibular functions in vitiligo patients

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    Eman Abd Elmohsin Dawoud

    2017-09-01

    Conclusion: The results in this study showed that 50% of vitiligo patients suffered from peripheral vestibular disorders in addition to auditory affection. Vitiligo patients require routine monitoring for auditory and vestibular functions for early identification and monitoring of changes as the disease progress.

  18. Serum Iron and Ferritin Levels in Patients with Vitiligo

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    Ayşe Tülin Mansur

    2010-10-01

    Full Text Available Background and Design: In recent years, the role of oxidative stress in vitiligo has been widely investigated. Iron and ferritin have important roles in inflammation and oxidative reactions. However, up to date, there are very limited studies on iron metabolism in patients with vitiligo. In this study, we aimed to investigate serum iron and ferritin levels in patients with localized and generalized vitiligo in comparison with a control group.Material and Method: The study groups comprised 68 patients with vitiligo who did not receive systemic treatment or phototherapy in the preceding month, and 72 age- and sex-matched patients with skin disorders other than vitiligo including tinea pedis, melanocytic nevi, and keratoses. Blood samples for serum iron and ferritin levels were obtained before breakfast, after verbal informed consent.Results: No statistically significant differences were found between vitilligo patients and control population with regard to serum levels of iron and ferritin (p=0.478, p=0.307. Patients with localized and generalized vitiligo were also similar for these parameters (p=0.054, p=0.867. Moreover, serum levels of iron and ferritin did not show any significant correlation with disease duration (p=0.382, p=0.485. Conclusion: Our results showed that serum iron and ferritin levels were similar in patients with vitiligo and control subjects. Further studies determining the skin levels of iron and ferritin may elucidate the probable role of these molecules in vitiligo.

  19. High Frequency Of Thyroid Dysfunction In Indian Patients With Vitiligo

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    Dave Shriya

    2003-01-01

    Full Text Available This Study was carried out look for any association of vitiligo with autoimmune thyroid disease and to find out clinical characteristics of vitiligo, which may predict such and association. Thirty-five consecutive cases of vitiligo were enrolled for this study. Besides recording the clinical features of vitiligo and thyroid disease, antithyroid autoantibody assays (anthi-thyroglobulin, anti-TPO and thyroid hormone profiles were done in these cases and 30 appropriately age and sex matched controls. Radioactive iodine uptake (RAIU thyroid scintiscan was done for all the cases and controls. Amongst the vitiligo cases, vitiligo vulgaris was the commonest type (45.70%, while mucosal vitiligo constituted 22.8% of cases. No Patient was found to have thyroid disease clinically; however, on assays, thyroid abnormality (endocrine, immunological or both was found in 57.1% of the cases as against 10% of the controls (p<0.05. Autonatibody positivity was found in 31.4% of the cases as against 10% of the controls (p<0.05. Biochemical abnormality (predominantly hyperthyroidism was found in 40% of the cases as against 6.7% of the controls (p<0.05. Four patients with hyperthyroidism showed diffuse increase in uptake on RAIU scan and auto antibody poisitivity, thus were diagnosed as Grave’s disease. An early age at onset was found in patients with thyroid disease. Mucosal vitiligo was found in 35% of the cases with thyroid dysfunction as against 6.7% in those without (p<0.05 with was the most unique finding of the study. To conclude, we would like to stress that patients with vitiligo should be screened for thyroid dysfunction, especially those with mucosal vitiligo.

  20. Regulatory T-cell cytokines in patients with nonsegmental vitiligo.

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    Kidir, Mehtap; Karabulut, Ayse A; Ercin, Mustafa E; Atasoy, Pınar

    2017-05-01

    In the etiopathogenesis of vitiligo, the role of suppressor cytokines, such as transforming growth factor-β (TGF-β) and interleukin-10 (IL-10), associated with regulatory T-cells (Treg) is not completely known. In this study, the role of Treg-cell functions in the skin of patients with nonsegmental vitiligo was investigated. Lesional and nonlesional skin samples from 30 adult volunteers ranging in age from 18 to 36 years with nonsegmental vitiligo were compared with normal skin area excision specimens of 30 benign melanocytic nevus cases as controls. All samples were evaluated staining for forkhead box P3 (Foxp3), TGF-β, and IL-10 using the standardized streptavidin-biotin immunoperoxidase immunohistochemistry method. Foxp3 expression was lower in lesional vitiligo skin specimens compared to controls; it was also lower in lesional vitiligo specimens than nonlesional vitiligo specimens. IL-10 levels were lower in lesional vitiligo specimens compared to the controls, whereas IL-10 expression was significantly lower in lesional specimens compared with nonlesional specimens. TGF-β expression was higher in both lesional and nonlesional skin specimens of patients with vitiligo compared to controls. TGF-β expression was lower in lesional skin specimens than nonlesional skin specimens. In addition, there was no significant correlation between Foxp3 expression with TGF-β and IL-10 expressions in lesional skin specimens in the vitiligo group. In this study, results supporting the contribution of Treg cells and IL-10 deficiency to the autoimmune process were obtained. Therefore, future studies are necessary to demonstrate the definitive role of Treg-cell functions in the etiopathogenesis of vitiligo.

  1. Quality of life in vitiligo patients.

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    Teovska Mitrevska, Natasa; Eleftheriadou, Viktoria; Guarneri, Fabrizio

    2012-01-01

    Quality of life is defined by the World Health Organization as "individuals' perceptions of their position in life in the context of the culture and value systems in which they live and in relation to their goals, expectations, standards and concerns." Often overlooked in the past, it is nowadays considered, in a more holistic view of medicine, a decisive factor to understand the impact of diseases and improve the quality of medical care. Such evaluation is particularly relevant for dermatological diseases, because visibility of the lesions can significantly affect self-esteem and social relationships. Vitiligo represents an emblematic case: often disfiguring and located in visible areas, confused in the past (and, in many world regions, even in the present) with leprosy, often perceived by physicians as a harmless, purely cosmetic problem, it significantly decreases the quality of life of affected persons. After a brief overview on definition, usefulness and methods for the assessment of quality of life, the authors examine the peculiarities of its relationship with skin diseases, particularly vitiligo. The state of the art of knowledge and research in this field is presented, together with data showing usefulness and positive results of a multidisciplinary approach, which adequately keeps into account perceived quality of life, on patient's satisfaction, adherence to treatment protocols and, ultimately, better outcome of treatments. In this context, an important role can be played by support communities, groups of patients and dedicated associations and societies, connected through modern communication networks like the Internet. © 2012 Wiley Periodicals, Inc.

  2. Transcriptional Analysis of Vitiligo Skin Reveals the Alteration of WNT Pathway: A Promising Target for Repigmenting Vitiligo Patients.

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    Regazzetti, Claire; Joly, Florence; Marty, Carine; Rivier, Michel; Mehul, Bruno; Reiniche, Pascale; Mounier, Carine; Rival, Yves; Piwnica, David; Cavalié, Marine; Chignon-Sicard, Bérengère; Ballotti, Robert; Voegel, Johannes; Passeron, Thierry

    2015-12-01

    Vitiligo affects 1% of the worldwide population. Halting disease progression and repigmenting the lesional skin represent the two faces of therapeutic challenge in vitiligo. We performed transcriptome analysis on lesional, perilesional, and non-depigmented skin from vitiligo patients and on matched skin from healthy subjects. We found a significant increase in CXCL10 in non-depigmented and perilesional vitiligo skin compared with levels in healthy control skin; however, neither CXCL10 nor other immune factors were deregulated in depigmented vitiligo skin. Interestingly, the WNT pathway, which is involved in melanocyte differentiation, was altered specifically in vitiligo skin. We demonstrated that oxidative stress decreases WNT expression/activation in keratinocytes and melanocytes. We developed an ex vivo skin model and confirmed the decrease activation of the WNT pathway in human skin subjected to oxidative stress. Finally, using pharmacological agents that activate the WNT pathway, we treated ex vivo depigmented skin from vitiligo patients and successfully induced differentiation of resident stem cells into pre-melanocytes. Our results shed light on the previously unrecognized role of decreased WNT activation in the prevention of melanocyte differentiation in depigmented vitiligo skin. Furthermore, these results support further clinical exploration of WNT agonists to repigment vitiligo lesions.

  3. Clinical and patient reported outcomes in vitiligo

    NARCIS (Netherlands)

    Linthorst Homan, M.W.

    2012-01-01

    Vitiligo is een stoornis die tot gevolg heeft dat door pigmentverlies de aangedane huid wit wordt. Hoewel in westerse landen de ziekte als onschuldig wordt gezien, is de kwaliteit van leven van een patiënt met vitiligo minder in vergelijking met de algemene bevolking. Bovendien heeft een vijfde van

  4. Transplantation of autologous noncultured epidermal cell suspension in treatment of patients with stable vitiligo

    Institute of Scientific and Technical Information of China (English)

    XU Ai-e; WEI Xiao-dong; CHENG Dong-qing; ZHOU He-fen; QIAN Guo-pei

    2005-01-01

    @@ Treatment of vitiligo by transplantation of noncultured melanocytes containing keratino-cytes has been successful since 1992,1 We report the encouraging results of autologous epidermal cell suspension in the treatment of 24 patients with stable vitiligo since 1998.

  5. Circulatory levels of antioxidants and lipid peroxidation in Indian patients with generalized and localized vitiligo.

    Science.gov (United States)

    Khan, Rehan; Satyam, Abhigyan; Gupta, Somesh; Sharma, Vinod K; Sharma, Alpana

    2009-10-01

    Vitiligo is an acquired skin disease, characterized by white areas on the skin due to loss of functional melanocytes. The pathogenesis of the disease is still unclear. Published data show the involvement of oxidative stress in the pathophysiology of vitiligo. A total of 30 vitiligo patients and 30 healthy controls were included in this study. We estimated serum levels of malondialdehyde (MDA), vitamins E and C, total antioxidant activity and whole blood levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in vitiligo patients and controls. We found significantly higher levels of MDA and significantly lower levels of SOD, GPx, vitamins C and E and total antioxidant activity in vitiligo patients compared with controls. This study is a maiden attempt to report on antioxidant parameters of both generalized/localized-type Indian vitiligo patients. Our results confirmed that oxidative stress may play an important role in the pathogenesis of vitiligo and cause melanocyte damage in vitiligo.

  6. Pediatric vitiligo.

    Science.gov (United States)

    Silverberg, Nanette B

    2014-04-01

    Vitiligo is a disease of pigment loss. Most investigators currently consider vitiligo to be a disorder that occurs as a result of autoimmune destruction of melanocytes, supported by identification of antimelanocyte antibodies in many patients, and the presence of comorbid autoimmune disease in patients with and family members of individuals with vitiligo. One-half of vitiligo cases are of childhood onset. This article presents a current overview of pediatric vitiligo including comorbidities of general health, psychological factors, therapeutic options, and long-term health considerations.

  7. Beliefs and perceptions of Arab vitiligo patients regarding their condition.

    Science.gov (United States)

    AlGhamdi, Khalid M

    2010-10-01

    Patients' beliefs about their illness can result in positive and/or negative implications for the management of their disease. To examine the beliefs held by vitiligo patients about their condition and to explore the potential factors that might influence such beliefs. The illness perception questionnaire (IPQ) was translated into the particular regional language (Arabic) and administered to adult vitiligo patients visiting our Dermatology Department, from 2006 to 2008. Demographic and illness details were obtained. We recruited 164 vitiligo patients (91 male subjects). The mean age was 27 years (SD = 13, range 15–57 years). It was believed that stress, altered immunity, and heredity were the cause of vitiligo by 33%, 26%, and 24% of the respondents respectively. Eighty-four percent and 28% believed in fate and evil eye as a cause of their illness respectively. Forty-two percent believed that their illness had a major effect on their lives and 44% believed that vitiligo had strongly affected the way others see them. For both beliefs, this was independent of the gender. Thirty-seven percent reported that they do not understand their illness. Six percent believed that their disease was contagious while 12% were not sure. Fifty-four and 57% reported feeling depressed and anxious respectively, because of their disease. This was more common in female patients (P = 0.019 and 0.031 respectively). Misconceptions and negative attitudes are widespread among vitiligo patients. The lack of disease-related understanding and the existence of anxiety and depression require targeted health education and psychosocial support. © 2010 The International Society of Dermatology.

  8. Thyroid dysfunction and thyroid antibodies in Iranian patients with vitiligo

    Directory of Open Access Journals (Sweden)

    Moradi Sedighe

    2008-01-01

    Full Text Available Vitiligo is a common skin disorder, and the pathogenesis is unknown. An increased prevalence of autoimmune thyroid diseases has been described in these patients. The aim of this study is to assess the prevalence of thyroid dysfunction and hypoparathyroidism in patients with vitiligo. Materials and Methods: One hundred and nine patients (38 males and 71 females with vitiligo were enrolled. Thyroid physical examination was carried out. Thyroid function tests, thyroid antibodies, calcium and phosphorus were assessed. The collected data were analysed by SPSS version 11. Results: Thyromegaly was found in 30.1% of patients. Hypothyroidism was found in 16 (15.7% out of 109 cases. Two of them had clinical and 14 had subclinical hypothyroidism. One patient had Grave′s disease. Antibody positivity was the most common disorder (anti-TPO and anti-tg were positive in 36.7 and 32.1%, respectively. No patient had hypoparathyroidism. Conclusion: According to our study, thyroid dysfunction, particulary hypothyroidism and thyroid antibodies increase in patients with vitiligo. We recommend thyroid antibodies assessment and thyroid function evaluation in these patients.

  9. Decreased copper and zinc in sera of Chinese vitiligo patients: a meta-analysis.

    Science.gov (United States)

    Zeng, Qinghai; Yin, Jun; Fan, Fan; Chen, Jing; Zuo, Chengxin; Xiang, Yaping; Tan, Lina; Huang, Jinhua; Xiao, Rong

    2014-03-01

    Abnormalities of copper (Cu) and zinc (Zn) are involved in the etiology and pathogenesis of vitiligo. However, controversial results exist now on Cu and Zn in serum of vitiligo patients. The purpose of this study is to compare the serum levels of Cu and Zn between vitiligo patients and healthy controls. In the meta-analysis, 16 studies with a total of 891 vitiligo cases and 1682 healthy controls were included. The levels of serum Cu and Zn were compared between groups of case and control. The serum levels of Cu were significantly lower in vitiligo patients than in healthy controls (Z = 4.04, P vitiligo patients than in healthy controls (Z = 4.88, P vitiligo patients. This may offer support for clinical administration of oral Cu and Zn supplements.

  10. Vitiligo

    Science.gov (United States)

    ... of the Year Award Arnold P. Gold Foundation Humanism in Medicine Award Diversity Mentorship Program Eugene Van ... in Dermatology JAAD Mohs AUC MyDermPath+ Psoriasis Patient education resources Practice Management Center Coding and reimbursement Coding ...

  11. QUALITY OF LIFE IN MALE AND FEMALE VITILIGO PATIENTS

    Directory of Open Access Journals (Sweden)

    Sharath Kumar

    2013-11-01

    Full Text Available ABSTRACT: BACKGROUND: Vitiligo is a common, acquired discoloration of the skin, characterized by w ell - circumscribed ivory or chalky white macules which are flush to the skin surface 1 . It affects atleast 0.5 - 1% of the total population 2 . It is commonly distributed in the peri - orificial areas, trunk, extensor surface of extremities, flexor wrists and axil lae. It results from a cellular autoimmune phenomenon that causes destruction of melanocytes. AIMS: To compare the quality of life between vitiligo patients and healthy individuals and to compare the quality of life between males and females vitiligo patie nts. MATERIALS AND METHODS: A comparative study of Twenty eight patients with vitiligo attending the Out Patient Department of Skin and STD department at KIMS Hospital, Bangalore was done between April and May 2013. RESULTS: A total of 13 male patients, 15 female patients and 15 male controls and 15 female controls, all within the age group of 16 - 50 years were taken and their DLQI scores were tabulated and analysed as follows: The mean and standard deviation of scores for all patients is 5.14±4.44, and that for controls is 0. This shows higher impairment in quality of life for patients as compared to controls and the results are statistically significant (Z value=2.08 and p value<0.05. CONCLUSION: 1. Quality of life was impaired in vitiligo patients. 2. Amo ng the patients, females had poorer quality of life. 3. Quality of life was more impaired in younger patients. 4. Impairment of quality of life was higher in unmarried patients than among married patients

  12. The psychological impact of vitiligo in adult Sudanese patients

    African Journals Online (AJOL)

    affecting 0.1-2% of the world's population, irrespective of race and gender.1 It ... to a severe loss of self-confidence and social anxiety, ... Adult patients of either gender. 3. .... Handa S, Dogra S. Epidemiology of childhood vitiligo: A study of 625.

  13. Oxidative stress level and tyrosinase activity in vitiligo patients

    Directory of Open Access Journals (Sweden)

    Eskandani M

    2010-01-01

    Full Text Available Background: Vitiligo is an acquired pigmentary disorder of the skin. Genetic factors, oxidative stress, autoimmunity, and neurochemical agents might be contributing factors for the development of the disease. Aims: To evaluate the oxidative stress level and tyrosinase activity in vitiligo patients and to compare them with healthy volunteers. Materials and Methods: We used Comet assay to evaluate DNA strand breaks in peripheral blood cells of active vitiligo patients. We then extracted total protein from lesional and nonlesional skin of ten selected patients. Tyrosinase activity was found to play a crucial role in melanogenesis. Results: The basal level of systemic oxidative DNA strand breaks in leukocytes increased in vitiligo patients compared to healthy participants. We observed that tyrosinase activity in lesional skin was lower than in nonlesional skin. Conclusion: Our finding suggests that increased levels of oxidative stress might impact tyrosinase activity and eumelanin synthesis via anabolism pathway of melanin synthesis. In sum, we observed a negative correlation between levels of systemic oxidative stress and of tyrosinase activity.

  14. Serum IL-23 levels in patients with vitiligo

    Directory of Open Access Journals (Sweden)

    Fatma Pelin Cengiz

    2014-12-01

    Full Text Available Background and Design: Interleukin-23 (IL-23 is a cytokine which is believed to have an important role in the pathogenesis of autoimmune diseases. Therefore, it can be used as a target molecule in the treatment of autoimmune diseases. The aim of our study was to determine serum IL-23 levels in patients with vitiligo to understand their possible roles in the disease etiology and to compare the results with the healthy controls. Materials and Methods: Fifty-one patients, who applied to the dermatology outpatient clinic and were diagnosed with vitiligo clinically and by wood lamp inspection, were enrolled in our study. Thirty-six age- and sex-matched healthy subjects were also enrolled as the control group. Serum IL-23 levels were studied in peripheral venous blood samples. Statistical analyses were made by SPSS version 16.0 for Windows. Results: Vitiligo group and control group were similar in terms of age and sex. Generalized type vitiligo was detected in 27 (52,9%, whereas focal, acrofacial, segmental and universal forms were detected in 13 (25,5%, 5 (9,8%, 3 (5,9% and 3 (5,9% of the patients, respectively. Mean serum IL-23 values were not significantly different between patient and control group (p=0.606. We also showed that serum IL-23 levels did not show any statistically significant difference in patients according to the size of the body surface area involved, involvement of the anogenital area, clinical manifestation, duration of disease and gender respectively (p=0.904, p=0.335, p=0.354, p=0.317, p=0.729, respectively. Conclusions: To our knowledge, this is the first study investigating IL-23 levels in vitiligo. According to our findings, IL-23 level was not higher in vitiligo patients. Because of the small number of subjects involved in our study, further studies involving larger groups are needed to verify our results in IL-23 levels in vitiligo patients.

  15. Knowledge, beliefs, and perceptions of Turkish vitiligo patients regarding their condition*

    Science.gov (United States)

    Topal, Ilteris Oguz; Duman, Hatice; Goncu, Ozgur Emek Kocaturk; Durmuscan, Mustafa; Gungor, Sule; Ulkumen, Pelin Kuteyla

    2016-01-01

    BACKGROUND Vitiligo is an acquired pigmentary skin disorder that affects 0.5% to 2.0% of the population. OBJECTIVE Patients' knowledge, opinions, and attitudes about vitiligo were evaluated. METHODS The team conducted a cross-sectional, descriptive, prospective study between June 2014 and May 2015. The study included 100 patients aged over 12 years who were diagnosed with vitiligo. A questionnaire including items on knowledge, opinions, and beliefs about vitiligo and the Illness Perception Questionnaire (IPQ) were filled out by the patients, and the results were analyzed. RESULTS In total, 100 (58 female, 42 male) patients were included in the study. Of them, 74% knew the name of their disease, 90% thought that vitiligo was not contagious, 48% reported that they obtained information on the disease from a doctor, and 69% believed they had adequate information on vitiligo. Eighty percent reported no negative effects from vitiligo on relationships with friends or family. It was believed that stress, excessive sun exposure, and heredity were causes of vitiligo, according to 84%, 37%, and 22% of the patients, respectively. Thirty-six patients (36%) believed that their illness was a serious disease and 35% deemed that it did not have a major impact on their lives. CONCLUSIONS Our results show that vitiligo patients were generally highly aware of their condition. The disease did not negatively affect patient opinions or attitudes about vitiligo. The authors believe that improving patient-physician communication will impact positively on the course of the disease. PMID:28099599

  16. Effect of an indigenous herbal combination on the melanin pigmentation activity in vitiligo patients.

    OpenAIRE

    Yadav NR; SC Gupta; Singh KG; Tripathi UC

    1994-01-01

    ABSTRACT: Ninteen of the twenty three vitiligo patients showed 30 to 95 percent skin repigmentation during forty eight weeks herbal therapy period when examined irrespective of different hypotheses concerning the pathogenesis of the skin depigmentation including vitiligo. More than 50 percent of the vitiligo patients under the trial depicted a speedy and well marked skin repigmentation ranging from 45 to 95 percent by the herbal drugs under examination. Whereas one patient showed a hundred pe...

  17. Evaluation of ocular findings in patients with vitiligo.

    Science.gov (United States)

    Karadag, Remzi; Esmer, Oktay; Karadag, Ayse S; Bilgili, Serap Gunes; Cakici, Ozgur; Demircan, Yuhanize Tas; Bayramlar, Huseyin

    2016-03-01

    In this study, we aimed to investigate ocular manifestations in patients with vitiligo. Sixty-one patients with vitiligo were included in the study. From the patients who referred for examination to the dermatology and ophthalmology clinic, 57 patients without any systemic disease were taken as the control group. In both groups, otorefractometry, keratometry, visual acuity test, intraocular pressure measurement, anterior segment, and fundus examinations of the eye with slit lamp, Schirmer test, and perimetry were carried out. The mean age was 24.54 ± 11.90 years and 23.03 ± 8.72 years in the patients and control group, respectively. The mean Schirmer test results were as follows: 16.74 ± 9.11 mm and 17.64 ± 9.41 mm for the right and left eyes of the patients, and 21.96 ± 12.51 mm and 23.42 ± 12.51 mm for the right and left eyes of controls, respectively. Of the patients, 36 eyes showed lenticular findings. However, only 12 eyes of the controls have some lenticular findings. Twenty-nine eyes in the vitiligo group and four in the controls showed some fundus findings. When the two groups were compared with each other, there was a statistically significant difference between them in terms of Schirmer test results, lens, and fundus findings (P  0.05 for all). Patients with vitiligo may have more lenticular and retinal findings than normal. They can be more prone to dry eye syndrome as well.

  18. Serum levels of folic acid and vitamin B12 in Korean patients with vitiligo.

    Science.gov (United States)

    Kim, S M; Kim, Y K; Hann, S K

    1999-06-01

    The association of vitiligo and pernicious anemia has been previously documented. The low levels of folic acid and vitamin B12 were thought to be related to vitiligo. To date, there have been very few reports about the serum levels of folic acid and vitamin B12 in patients with vitiligo. Using radioimmunoassay, we measured the serum levels of folic acid and vitamin B12 in 100 Korean patients with vitiligo. The mean serum levels of folic acid and vitamin B12 were 6.31 +/- 2.82 ng/ml and 630.25 +/- 230.94 pg/ml, respectively, in patients with vitiligo. These levels showed no significant difference compared to the normal control group, suggesting that folic acid and vitamin B12 do not appear to play a role in the pathogenesis of vitiligo.

  19. The psychosocial impact of vitiligo in Indian patients

    Directory of Open Access Journals (Sweden)

    Pooja Pahwa

    2013-01-01

    Full Text Available Background: Vitiligo has a special significance in Indian patients both because depigmentation is obvious on darker skin and the enormous stigma associated with the disease in the culture. Aims: This study was carried out to determine the beliefs about causation, aspects of the disease that cause concern, medical, and psychosocial needs of the patients, expectation from treatment and from the treating physician, and effects of disease on the patient′s life. Methods: Semi-structured interviews were conducted in 50 patients with vitiligo. Purposive sampling was used to select subjects for the study. Each interview was recorded on an audio-cassette and transcripts were analyzed to identify significant issues and concerns. Results: Patients had a range of concerns regarding their disease such as physical appearance, progression of white patches onto exposed skin and the whole body, ostracism, social restriction, dietary restrictions, difficulty in getting jobs, and they considered it to be a significant barrier to getting married. The condition was perceived to be a serious illness. Stigma and suicidal ideation was reported. While there were several misconceptions about the cause of vitiligo, most patients did not think their disease was contagious, heritable or related to leprosy. Multiple medical consultations were frequent. Complete repigmentation was strongly desired, but a lesser degree of repigmentation was acceptable if progression of disease could be arrested. The problems were perceived to be more severe in women. The disease imposed a significant financial burden. Conclusion: Addressing psychosocial factors is an important aspect of the management of vitiligo, particularly in patients from communities where the disease is greatly stigmatizing.

  20. A comparative study of oxidant-antioxidant status in stable and active vitiligo patients.

    Science.gov (United States)

    Ines, Dammak; Sonia, Boudaya; Riadh, Ben Mansour; Amel, El Gaied; Slaheddine, Marrekchi; Hamida, Turki; Hamadi, Attia; Basma, Hentati

    2006-09-01

    The pathogenetic mechanisms in vitiligo have not been completely clarified. One of the major hypotheses in the pathogenesis of vitiligo is the oxidative stress hypothesis. The active or stable phase of vitiligo is defined on the basis of the progression or appearance of new lesions in the last 3 months and the absence of new lesions or their progression in the last 6 months, respectively. Eighteen patients with active vitiligo, 18 patients with stable vitiligo, and 40 controls were included in this study. We examined serum levels of malondialdehyde, selenium, vitamin E and A, and the erythrocyte activities of glutathione peroxidase, superoxide dismutase, and catalase. Our results revealed a significantly higher level of serum malondialdehyde, selenium in patients with active disease compared with the controls. Significant higher increase in erythrocytes superoxide dismutase activities was observed in active vitiligo group, erythrocyte glutathione peroxidase activity was decreased significantly in active disease, whereas erythrocyte catalase activity and plasma vitamin E and A levels were not different in vitiligo patients as compared with controls. Our study shows that oxidative stress is involved in the pathophysiology of both active and stable vitiligo but increased imbalance of antioxidants was observed in the blood of active vitiligo patients.

  1. Oxidative stress in the blood of patients with active localized vitiligo:

    OpenAIRE

    2008-01-01

    Objectives: Vitiligo is an acquired skin disease characterized by white areas on the skin. The pathogenesis of the disease is still unclear. Some findings show that oxidative stress could be an important phenomenon in the pathophysiology of vitiligo. Methods: We evaluated 16 consecutive localized vitiligo patients and 16 healthy controls of a similar age and sex distribution. We measured their indicators of oxidative stress such as catalase (CAT), superoxide dismutase (SOD), glucose 6-phospha...

  2. Vaccine-specific local T cell reactivity in immunotherapy-associated vitiligo in melanoma patients.

    NARCIS (Netherlands)

    Jacobs, J.F.M.; Aarntzen, E.H.J.G.; Sibelt, L.A.G.; Blokx, W.A.M.; Boullart, A.C.I.; Gerritsen, M.J.P.; Hoogerbrugge, P.M.; Figdor, C.G.; Adema, G.J.; Punt, C.J.A.; Vries, I.J.M. de

    2009-01-01

    The occurrence of vitiligo in patients with melanoma is especially reported for patients undergoing immunotherapy. While vitiligo in these patients is thought to be related to an immune response directed against melanoma cells, solid evidence is lacking. Here we report local cytotoxic T cell reactiv

  3. Long-term results of 2-mm punch grafting in patients with vitiligo vulgaris and segmental vitiligo : effect of disease activity

    NARCIS (Netherlands)

    Fongers, A.; Wolkerstorfer, A.; Nieuweboer-Krobotova, L.; Krawczyk, P.; Toth, G. G.; van der Veen, J. P. W.

    2009-01-01

    Background Punch grafting is a simple and frequently used technique for the treatment of stable vitiligo, resistant to medical therapy. However, studies reporting long-term results are exceptional. Objectives To evaluate the long-term results of 2-mm punch grafting in patients with vitiligo vulgaris

  4. On the pathophysiology of vitiligo: Possible treatment options

    OpenAIRE

    2012-01-01

    Vitiligo is an acquired depigmenting disorder usually classified as non-segmental and segmental types with a higher incidence of the non-segmental ones. The cause of non-segmental vitiligo is still unknown. Currently, it is a dogma that there are several genes affecting the immune system and the pigment system that predisposes someone to develop vitiligo. A precipitating factor must then ellicit an interaction between the immune system and the melanocyte, resulting in destruction of the melan...

  5. Analysis of oxidative stress status, catalase and catechol-O-methyltransferase polymorphisms in Egyptian vitiligo patients.

    Directory of Open Access Journals (Sweden)

    Dina A Mehaney

    Full Text Available Vitiligo is the most common depigmentation disorder of the skin. Oxidative stress is implicated as one of the probable events involved in vitiligo pathogenesis possibly contributing to melanocyte destruction. Evidence indicates that certain genes including those involved in oxidative stress and melanin synthesis are crucial for development of vitiligo. This study evaluates the oxidative stress status, the role of catalase (CAT and catechol-O-Methyltransferase (COMT gene polymorphisms in the etiology of generalized vitiligo in Egyptians. Total antioxidant capacity (TAC and malondialdehyde (MDA levels as well as CAT exon 9 T/C and COMT 158 G/A polymorphisms were determined in 89 patients and 90 age and sex-matched controls. Our results showed significantly lower TAC along with higher MDA levels in vitiligo patients compared with controls. Meanwhile, genotype and allele distributions of CAT and COMT polymorphisms in cases were not significantly different from those of controls. Moreover, we found no association between both polymorphisms and vitiligo susceptibility. In conclusion, the enhanced oxidative stress with the lack of association between CAT and COMT polymorphisms and susceptibility to vitiligo in our patients suggest that mutations in other genes related to the oxidative pathway might contribute to the etiology of generalized vitiligo in Egyptian population.

  6. STUDY OF DERMATOLOGICAL QUALITY OF LIFE IN PATIENTS WITH VITILIGO ATTENDING THE OUT PATIENT DEPARTMENT OF DERMATOLOGY IN A TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Ashiq

    2015-11-01

    Full Text Available : BACKGROUND: Vitiligo is an autoimmune, chronic pigmentary disorder causing considerable amount of social stigma leading to low self-esteem. AIM: To assess the quality of life in patients with vitiligo who are attending the outpatient department in Sri Ramachandra Hospital. The study was done over a period of 2 years. Parameters that were studied included gender distribution, occupation, type of Vitiligo. METHODS: 200 vitiligo patients answered a ten point questionnaire designed by Cardiff to assess the quality of life. RESULTS: Out of the 200 patients, 66 were male & 134 female, 158 were married and 42 unmarried, vitiligo vulgaris being the commonest type. Mean DLQI was higher in housewife (Occupation-13.5, vitiligo vulgaris (Type of vitiligo-16.34, diabetes mellitus (Association with vitiligo-27. CONCLUSION: The results demonstrate that vitiligo has a very large to extremely large effect on the quality of life.

  7. Mitochondrial DNA acquires immunogenicity on exposure to nitrosative stress in patients with vitiligo.

    Science.gov (United States)

    Al-Shobaili, Hani A; Rasheed, Zafar

    2014-10-01

    Vitiligo is a common pigmentary skin disorder of unknown etiology. Many studies show the defective mitochondrial functionality in vitiligo patients, but the potential role of mitochondrial DNA (mtDNA) in the pathogenesis of vitiligo remains to be investigated. Recent evidences demonstrate that mitochondria possess their own nitric-oxide-synthase and can produce endogenous peroxynitrite (ONOO(-)). This study was undertaken to investigate the role of ONOO(-)-modified-mitochondrial-DNA (ONOO(-)-mtDNA) in vitiligo autoimmunity. Our data revealed that ONOO(-)-induced modifications in mtDNA caused structural alterations. Specificity of immunoglobulin G (IgG) from vitiligo patients (n=26) and controls (n=25) were analysed towards ONOO(-)-mtDNA. Vitligo-IgG samples (Vt-IgG) show preferential binding to ONOO(-)-mtDNA in comparison with native mtDNA (pvitiligo patients. Furthermore, levels of anti-ONOO(-)-mtDNA-IgG, inducible-nitric-oxide-synthase (iNOS), nitric oxide (NO) and nitrotyrosine were higher among vitiligo patients whose disease durations (DD) were ⩾5 years as compared to patients with lower DD (DDvitiligo patients. Our data provide an important insight into the immunological mechanisms occur in vitiligo. The ONOO(-)-mtDNA may be useful in elucidating the mechanisms of disease pathogenesis.

  8. Prevalência de psoríase em estudo de 261 pacientes com vitiligo Prevalence of psoriasis in a study of 261 patients with vitiligo

    Directory of Open Access Journals (Sweden)

    Caio César Silva de Castro

    2005-10-01

    Full Text Available FUNDAMENTOS: O estudo da associação de psoríase e vitiligo é necessário em razão das prováveis origens imunológicas dessas enfermidades e da proximidade de loci encontrados no cromossomo 1p31. OBJETIVO: O propósito principal foi determinar a prevalência de psoríase em pacientes com vitiligo em amostra de 740 pacientes submetidos à fototerapia em Curitiba, PR e descrever suas características clínicas. MÉTODOS: Dos 740, foram estudados retrospectivamente 261 pacientes com diagnóstico de vitiligo, e analisados aqueles com associação de vitiligo e psoríase no período entre 2000 e 2004. RESULTADOS: A prevalência dessa associação neste trabalho foi de 3,06%; semelhante à de outras pesquisas anteriores. Foram identificados dois casos dos quais não se encontrou relato em revisão da literatura: a a associação de vitiligo, psoríase e halo nevo; b a associação de vitiligo segmentar e psoríase. CONCLUSÕES: A associação de vitiligo e psoríase tem sido raramente relatada, sendo ainda necessários estudos sobre a fisiopatologia e a genética dessa associação.BACKGROUND: A study about the association of psoriasis and vitiligo is necessary due to possible immunologic origin of these diseases and their susceptibility loci found on chromosome 1p31. OBJECTIVE: The main objective was to determine the prevalence of psoriasis among patients with vitiligo in a sample of 740 patients submitted to phototherapy and to describe their clinical features. METHOD: Out of 740 patients, 261 were retrospectively studied and analyzed regarding the association of vitiligo and psoriasis, from 2000 to 2004. RESULTS: The prevalence of this association in our survey was 3.06%, which is similar to other previous studies. We identified two cases not reported in our literature review: a the association of psoriasis, vitiligo and halo nevus, b the association of segmental vitiligo and psoriasis. CONCLUSIONS: The association of vitiligo and psoriasis

  9. Topical melagenine for repigmentation in twenty-two child patients with vitiligo on the scalp

    Institute of Scientific and Technical Information of China (English)

    许爱娥; 尉晓冬

    2004-01-01

    Background The purpose of this study was to evaluate the efficacy of topical melagenine for repigmentation in child patients with vitiligo on the scalp. Methods Twenty-two child patients with vitiligo on the scalp were treated with 1.2 mg/ml aqueous melagenine in combination with 20 minutes of infrared exposure twice daily. Results In 4 patients (18.2%), melagenine treatment in combination with infrared exposure led to complete recovery; in 6 patients (27.3%), treatment was shown to be effective; in 8 patients(36.3%), treatment led to improvements in patient condition; and only 4 patients (18.2%) showed no response after 1 -2 treatment sessions. The general effective rate of melagenine-infrared combination treatment was 45. 5% for the children with vitiligo on the scalp, and treatment was accompanied by minimal side effects.Conclusion Melagenine may be efficacious and a safe treatment option for childhood vitiligo affecting the scalp.

  10. Study of neuropeptide Y in the plasma and skin tissue of patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    ZENG Wei-hui; TANG Sheng-shun; ZHENG Yan; LEI Xiao-bing

    2004-01-01

    Objective: To study the role of neuropeptide Y (NPY) in the pathogenesis of vitiligo. Methods: The levels of NPY in the plasma from patients with vitiligo and healthy volunteers were measured by 125 I RIA Kit. The expression of NPY in normal skin tissues, uninvolved tissues and lesional tissues of vitiligo was detected by immunohistochemistry. Results: The levels of NPY in the patients with vitiligo of all types were significantly higher than that in the normal controls. In all types, the levels in active stage were significantly higher than those in stable stage. The expression of NPY was upregulated in lesions of patients with active vitiligo ( P < 0.01) compared with those in normal skin tissues and uninvolved tissues.There was significant difference of NPY expression between active stage and stable stage (P < 0.01 ). Conclusion: These findings support the concept of neuropeptide involvement in vitiligo, especially in active vitiligo, and suggest that NPY may play a role in the pathogenesis of this disease.

  11. The Frequency of Autoimmune Thyroid Disease in Alopecia Areata and Vitiligo Patients

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    Gulcan Saylam Kurtipek

    2015-01-01

    Full Text Available Aim. Many studies demonstrated that alopecia areata (AA and vitiligo are commonly associated with autoimmune thyroid diseases. We aimed to investigate the frequency of thyroid dysfunctions and autoimmunity related with vitiligo and AA. Material and Methods. 200 patients, 92 AA and 108 vitiligo diagnosed, were surveyed retrospectively. The control population was in reference range and from Konya, central Anatolian region of Turkey. Thyroid function tests (free T3, free T4, and TSH and serum thyroid autoantibody (anti-TG, anti-TPO levels were evaluated in all patients. Results. In vitiligo patients, 9 (8.3% had elevated anti-TG levels and 16 (14.8% had elevated anti-TPO, and in 17 patients (15.7% TSH levels were elevated and 3 (2.8% patients had elevated fT4 levels and 5 (4.6% had elevated fT3 levels. Within AA patients, 2 (2.2% had anti-TG elevation and 13 (14.1% had anti-TPO elevation, in 7 patients (7.6% TSH were elevated, and in 1 patient (1.1% fT4 were elevated and 5 (5.4% patients had elevated fT3 levels. Conclusion. In our study, impaired thyroid functions and thyroid autoantibodies in vitiligo and AA patients were identified at lower rates than the previous studies. According to results of this study there is no need for detailed examination in alopecia areata and vitiligo patients without clinical history.

  12. Serum concentration of IL-6, IL-2, TNF-α, and IFNγ in Vitiligo patients

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    Suman Singh

    2012-01-01

    Full Text Available Background: Vitiligo is an acquired depigmenting disorder characterized by the loss of functional melanocytes from the epidermis. Although the etiology of vitiligo is unknown, over the last few years, substantial data from clinical research has greatly supported the ′Autoimmune theory′ and this is supported by the frequent association of vitiligo with disorders that have an autoimmune origin, including Hashimoto′s thyroiditis, Graves disease, type 1 insulin-dependent diabetes mellitus, and Addison′s disease. As cytokines are important mediators of immunity, there is evidence to suggest that they play a major role in the pathogenesis of autoimmune diseases. Aim: Keeping this in view we have assayed sera for cytokine IL-6, IL-2, Tumor necrosis factor (TNF-α, and IFNγ in 80 cases of vitiligo and compared it with healthy subjects, in order to find out whether they play a role in the pathogenesis of vitiligo or not. Materials and Methods: Serum IL-6, IL-2, TNF-α, and IFNγ were done by the indirect enzyme linked immunosorbent assay (ELISA. Results: The mean serum IL-6 and IL-2 levels in the patient group were significantly higher when compared with those of the normal controls. The mean serum IFNγ level in patients with vitiligo was significantly lower than that in the control group. There was no significant difference in the serum level of TNF-α between vitiligo and healthy controls. Conclusion : An increase in the production of proinflammatory cytokines such as IL-6 and IL-2 in vitiligo patients may play an important role in melanocytic cytotoxicity. Thus, we speculate that the cytokine production of epidermal microenvironment may be involved in vitiligo.

  13. Association of Interleukin-10 Gene Promoter Polymorphisms in Saudi Patients with Vitiligo

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    Abdullah Abanmi

    2008-01-01

    Full Text Available The promoter region of human Interleukin −10 gene is highly polymorphic and has been associated with numerous autoimmune diseases. Recent studies have linked vitiligo with defective autoimmune system. This study is aimed to explore a possible association between IL-10 gene polymorphism and vitiligo in Saudi population. This case control study consisted of 184 Saudi subjects including 83 vitiligo patients (40 males, 43 females mean age 27.85 ± 12.43 years and 101 matched controls. Genomic DNA was extracted from the blood samples of healthy controls and Vitiligo patients visiting out patient clinic of Department of Dermatology, Riyadh Armed Forces Hospital, using QIA ampR DNA mini kit (Qiagen CA, USA. Interleukin-10 gene was amplified by polymerase chain reaction (PCR using Arms primers to detect any polymorphism involved at positions −592, −819 and −1082.

  14. Vitiligo at the sites of irradiation in a patient with Hodgkin's disease

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    Pajonk, F.; Weissenberger, C.; Witucki, G.; Henke, M. [Radiological Univ. Clinic, Freiburg (Germany). Dept. of Radiation Therapy

    2002-03-01

    Background: Vitiligo is one of the most common skin disorders. However, the etiology of vitiligo is still unknown. Current hypotheses discuss autoimmune, autotoxic and neuronal mechanisms. Here we report the case of radiation-induced depigmentation of the skin of a patient with Hodgkin's disease and 25-year history of vitiligo. Patient and Method: We compared possible differences in skin color, skin moisture, microcirculation and skin elasticity between normal skin and skin exhibiting persistent depigmentation in a 37-year-old patient 40 months after completion of external beam radiotherapy. Results: Colormetrically we found a dose-dependent decrease of the red/green and yellow/blue saturation combined with an overall increase in brightness in depigmented skin when compared with normal skin. This was in agreement with a loss of melanocytes in vitiligo. Depigmentation was complete in areas receiving 40 Gy. Areas which received 30 Gy showed depigmentation only if the skin dose was increased by the loss of depth of the build-up dose region in areas with direct contact with the irradiation table. We could not show any change in skin moisture, microcirculation or skin elasticity. Conclusions: Complete radiation-induced depigmentation of skin from patients suffering from vitiligo is a side effect of radiation therapy. Patients should be informed about this side effect by the radiooncologist. Preventing the loss of depth of the build-up dose region might improve the cosmetic results of radiation therapy in patients with history of vitiligo. (orig.)

  15. The Efficacy Of Low-Dose Oral Corticosteroids In The Treatment Of Vitiligo Patients

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    Mirshams-Shahshahani M

    2005-05-01

    Full Text Available Background: Vitiligo is an acquired pigmentary disorder that affects 1% of population. It presents as depigmented patches. One of the most probable theories regarding the pathogenesis of vitiligo is autoimmunity. Systemic corticosteroids may arrest the progression of vitiligo and lead to repigmentation by suppressing immune system. The objective of this study is to assess the clinical efficacy of low-dose oral corticosteroids in actively progressing vitiligo. Materials and Methods: Seventy four patients with vitiligo were evaluated. The patients took daily doses of oral prednisolone (0.3 mg/kg initially for 2 months. Then the dosage was halved monthly, for the five subsequent months of treatment. The effects of treatment were evaluated using photography's before and after the study. Side effects were assessed at the first, second, third and fourth month of the treatment. Results: Arrested progression of vitiligo and repigmentation were noted in 74.3% and 62.1% of patients respectively. The mean pigmentation was 26.8%. The localized form, lower age of disease onset, no hair whiteness on the lesions and less affliction percent showed increased repigmentation with statistical significance. There was no significant difference between sexes and positive family history of vitiligo in patients. The best therapeutic results were obtained for facial lesions and the worst for mucosal lesions. The side effects of treatment were minimal and did not affect the course of the treatment. Conclusion: Low-dose oral corticosteroids are effective and have few serious side effects in preventing the progression of actively progressing vitiligo but regimentation is not significant and this regimen is effective in patients who are refractory to topical corticosteroids or phototherapy.

  16. Vitiligo patient-derived keratinocytes exhibit characteristics of normal wound healing via epithelial to mesenchymal transition.

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    Banerjee, Poulomi; Venkatachalam, Sandhyaa; Mamidi, Murali Krishna; Bhonde, Ramesh; Shankar, Krupa; Pal, Rajarshi

    2015-05-01

    Vitiligo is an autoimmune disorder that leads to depigmentation of skin via melanocyte dysfunction. Keratinocyte-induced toxicity is one among the several etiological factors implicated for vitiligo, and hence, autologous keratinocyte grafting is projected as one of the primary mode of treatment for vitiligo. However, reports indicate that perilesional keratinocytes not only display signatures of apoptosis but also could secrete cytokines and mediators which have antagonistic effect on proliferation or survival. Therefore, we investigated how vitiligo patients' derived keratinocytes respond to surplus amounts of inflammatory cytokines and whether they recapitulate events that take place during conventional wound healing. The primary objective of our study was to determine whether keratinocytes isolated from a vitiligo patient would undergo epithelial-mesenchymal transition similar to their normal counterparts upon induction with inflammatory cytokines such as TGF-b1 and EGF. We found that these keratinocytes undergo EMT during wound repair accompanied with increase in the levels of mesenchymal markers and ECM proteins; decrease in the levels of epithelial markers and enhanced migratory ability. Besides, we also demonstrated that EMT induction leads to activation of SMAD and MAPK pathways via Ras, Raf, PAI 1, Snail, Slug and ZO1. To our knowledge, this is the first report on the characterization of primary keratinocytes isolated from vitiligo patients with respect to their wound healing capacity. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. Revised classification/nomenclature of vitiligo and related issues: the Vitiligo Global Issues Consensus Conference.

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    Ezzedine, K; Lim, H W; Suzuki, T; Katayama, I; Hamzavi, I; Lan, C C E; Goh, B K; Anbar, T; Silva de Castro, C; Lee, A Y; Parsad, D; van Geel, N; Le Poole, I C; Oiso, N; Benzekri, L; Spritz, R; Gauthier, Y; Hann, S K; Picardo, M; Taieb, A

    2012-05-01

    During the 2011 International Pigment Cell Conference (IPCC), the Vitiligo European Taskforce (VETF) convened a consensus conference on issues of global importance for vitiligo clinical research. As suggested by an international panel of experts, the conference focused on four topics: classification and nomenclature; definition of stable disease; definition of Koebner's phenomenon (KP); and 'autoimmune vitiligo'. These topics were discussed in seven working groups representing different geographical regions. A consensus emerged that segmental vitiligo be classified separately from all other forms of vitiligo and that the term 'vitiligo' be used as an umbrella term for all non-segmental forms of vitiligo, including 'mixed vitiligo' in which segmental and non-segmental vitiligo are combined and which is considered a subgroup of vitiligo. Further, the conference recommends that disease stability be best assessed based on the stability of individual lesions rather than the overall stability of the disease as the latter is difficult to define precisely and reliably. The conference also endorsed the classification of KP for vitiligo as proposed by the VETF (history based, clinical observation based, or experimentally induced). Lastly, the conference agreed that 'autoimmune vitiligo' should not be used as a separate classification as published evidence indicates that the pathophysiology of all forms of vitiligo likely involves autoimmune or inflammatory mechanisms.

  18. Association of Angiotensin-Converting Enzyme (ACE) Gene Polymorphism with Inflammation and Cellular Cytotoxicity in Vitiligo Patients

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    Laila Rashed; Rania Abdel Hay; Rania Mahmoud; Nermeen Hasan; Amr Zahra; Salwa Fayez

    2015-01-01

    Background Vitiligo is a disorder with profound heterogeneity in its aetio-pathophysiology. Angiotensin converting enzyme (ACE) plays an important role in the physiology of the vasculature, blood pressure and inflammation. An insertion/deletion (I/D) polymorphism of the ACE gene was reported be associated with the development of vitiligo. Objective Our aim was to evaluate the ACE I/D polymorphism in vitiligo patients and controls. Our second aim was to find a possible association between ACE ...

  19. Childhood vitiligo

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    Aparna Palit

    2012-01-01

    Full Text Available Childhood vitiligo is often encountered in dermatological practice. When present in infancy or early childhood, various nevoid and hereditary disorders are to be differentiated. In many cases, familial aggregation of the disease is seen and other autoimmune disorders may be associated. Segmental presentation is more common, and limited body surface area involvement is usual in this age group. Children with vitiligo often suffer from anxiety and depression because of their unusual appearance. Management of vitiligo in children is difficult as therapeutic options are restricted when compared to that in adult patients. Selection of treatment should be careful in these patients with the aim to achieve best results with minimal side effects as well as relieving patients′ and parents′ anxiety.

  20. A clinical study of vitiligo

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    Martis Jacintha

    2002-01-01

    Full Text Available A clinical study of one hundred patients having vitiligo revealed the incidence among new patients to be 1.84%. The male/female ratio was 1:1.22. Family history of vitiligo was available in 8% of our patients. The different morphological pattern consisted of vitiligo vulgaris (39 cases, focal vitiligo (27 cases, acrofacial vitiligo (18 cases, lip - tip vitiligo (7 cases, lip vitiligo (5 cases, segmental vitiligo (3 cases and universal vitiligo (1 case. Associated diseases include atopic dermatitis (2 cases, Hansen s disease (2 cases, alopecia areata (1 case, halo naevus (1 case, chronic urticaria (1 case, lichen planus (1 case, diabetes mellitus (9 cases, hypertension (4 cases, hypothyroidism (2 cases, epilepsy (1 case and IHD (1 case.

  1. Clinical Profile of Vitiligo in North India.

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    Koranne, R V; Sehpgal, V N; Sachdeva, K G

    1986-01-01

    A clinical analysis of L50 patients having vitiligo revealed the incidence among new patients to be 1.25%. The male /female ratio was 1.3:1. Patients below the age of 30 years constituted 85%. Family history of vitiligo was available in 240/o. The different morphologic, Patterns consisted of vitiligo vulgaris (90 cases), vitiligo areata (22 cases), vitiligo acro-facialis (11 cases), vitiligo univers (11 cases , vitiligo mucoaae (9 cases),and vitiligo zosteriformis (7 cases). Association with diabetes meffitus (5 cases), alopecia areata (4 cases), Hashimoto's thyroiditis (1 case) and pemphigus vulgaris ( 1 case ) was observed.

  2. The antibody response against MART-1 differs in patients with melanoma-associated leucoderma and vitiligo.

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    Teulings, Hansje-Eva; Willemsen, Karin J; Glykofridis, Iris; Krebbers, Gabrielle; Komen, Lisa; Kroon, Marije W; Kemp, E Helen; Wolkerstorfer, Albert; van der Veen, J P Wietze; Luiten, Rosalie M; Tjin, Esther P M

    2014-11-01

    Patients with melanoma may develop skin depigmentation spontaneously or following therapy, referred to as melanoma-associated leucoderma (MAL). As clinical presentation of MAL may precede primary/metastatic melanoma detection, recognition of MAL is important to prevent its misdiagnosis as vitiligo and the subsequent application of immunosuppressive treatment. To reveal the immunity involved in MAL development, we investigated the presence of antibody and T-cell immune responses directed against the melanocyte-differentiation-antigens MART-1 (Melan-A), tyrosinase and gp100 in patients with MAL, as compared to patients with vitiligo. Autoantibodies to gp100 and tyrosinase were commonly found in both diseases. Interestingly, MART-1 antibodies were only present in patients with MAL. Melanocyte antigen-specific T cells were found in all patients, with relatively more specific T cells in patients with active vitiligo. Although MAL and vitiligo may appear clinically similar, our results indicate that the humoral immune responses against MART-1 differ between these diseases, which can help to differentiate MAL from vitiligo.

  3. The Effect of Otic Melanocyte Destruction on Auditory and Vestibular Function: a Study on Vitiligo Patients

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    Parvane Mahdi

    2016-03-01

    Full Text Available The hallmark of vitiligo is the disappearance of melanocytes from the skin. As a result, of melanocytes presence in the auditory and vestibular apparatus, the involvement of these systems in vitiligo which targets the melanocytes of the whole body is possible; suggesting that vitiligo is a systemic disease rather than a purely cutaneous problem. A total of 21 patients with vitiligo were enrolled in this study. A group of 20 healthy subjects served as a control group. Pure tone audiometry (PTA, auditory brainstem responses (ABR and vestibular evoked myogenic potentials (VEMP were carried out in all participants. High frequency sensory neural hearing loss was seen in 8 (38.09% patients. ABR analysis revealed 10 (47.61% had an abnormal increase in latency of wave III, and 6 (28.57% had an abnormal prolongation of IPL I-III, however, regarding our VEMP findings, there were no recorded responses on left ear of 1 (4.76% patient and latency of p13 was prolonged in 5(23.80% patients. There was no correlation between ages, duration of disease, and any of the recorded parameters (P>0.05. In the present survey, we highlighted the auditory and vestibular involvement in vitiligo patients.

  4. The efficacy of low-dose oral corticosteroids in the treatment of vitiligo patient

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    Banerjee K

    2003-03-01

    Full Text Available Autoimmunity is one of the most probable pathogenesis of vitiligo. Systemic corticosteroids may arrest the progression of vitiligo and lead to repigmentation by suppressing immunity. The clinical efficacy of low-dose oral corticosteroids was assessed to minimize the side-effects in actively spreading vitiligo patients. One hundred (100 patients with vitiligo were evaluated. The patients took daily doses of oral prednisolone (0.3mg/kg body weight initially as a single oral dose after breakfast for the first 2 months. The dosage was then reduced to half the initial dose during the 3rd month and was halved again for the 4th and final month. After 4 months of treatment, 76% showed repigmentation while the arrest of progression (both repigmentation and stationary was noted in 90% of patients. Male sex, and patients under 15 years of age showed pronounced repigmentation with statistical significance. According to this study low-dose oral prednisolone is an effective method in preventing progression and inducing repigmentation of fast-spreading vitiligo without the associated serious side-effects.

  5. [Peculiarities of cytokine status in patients with vitiligo and stress in anamnesis].

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    Tsiskarishvili, N V; Katsitadze, A; Tsiskarishvili, N I; Chitanava, L

    2014-10-01

    The aim of our study was to investigate the content of some proinflammatory cytokines (IL-2, IL-6) in the serum of patients with vitiligo and stress in anamnesis. Cytokine levels in serum were measured in 50 patients with vitiligo (20 men and 30 women aged from 18 to 65 years old.) All the patients were divided according to anamnesis (27 patients with stress in anamnesis and 23 patients, where the onset of the disease was not preceded by stress). Patients of both groups were similar in terms of shape (according to T. Fitzpatrick classification) and in terms of the dermatosis stages. Concomitant autoimmune or inflammatory diseases of the skin in our vitiligo patients were not observed. The cytokines in the blood serum of vitiligo patients were determined by ELISA using the corresponding monoclonal antibodies. Student's t-test was used for evaluation of statistical significance of received results (p vitiligo, in which stress was not preceded dermatosis, was significantly higher than that in healthy individuals (25,5 ± 7,6 vs 10,5 ± 3,6 pg/ml, p vitiligo patients was a similar to changes in IL-2 content, and the most significant this indicator was in patients with generalized and progressive stage of the disease. Data on increased concentrations of IL-6 agree with the majority of studies conducted earlier. For a complete analysis of our results on the reduction of the cytokines (IL-2, IL-6) content in patients with a stress in anamnesis, that will allow to identify the most effective ways to treat patients with this pathology (including their cytokine status), further research is necessary.

  6. Nonsegmental Vitiligo and Autoimmune Mechanism

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    Naoki Oiso

    2011-01-01

    Full Text Available Nonsegmental vitiligo is a depigmented skin disorder showing acquired, progressive, and depigmented lesions of the skin, mucosa, and hair. It is believed to be caused mainly by the autoimmune loss of melanocytes from the involved areas. It is frequently associated with other autoimmune diseases, particularly autoimmune thyroid diseases including Hashimoto's thyroiditis and Graves' disease, rheumatoid arthritis, type 1 diabetes, psoriasis, pernicious anemia, systemic lupus erythematosus, Addison's disease, and alopecia areata. This indicates the presence of genetically determined susceptibility to not only vitiligo but also to other autoimmune disorders. Here, we summarize current understanding of autoimmune pathogenesis in non-segmental vitiligo.

  7. A measurement of the stigma among vitiligo and psoriasis patients in India

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    Rajan Pichaimuthu

    2011-01-01

    Full Text Available Background and Aim: The aim is to measure and to compare the level of social participation experienced by vitiligo and psoriasis patients in their domestic and social life in an Indian context. Methods: A cross-sectional comparative study with a sample of 150 cases each of psoriasis and vitiligo, a total of 300 subjects. A detailed clinical assessment of these two conditions, including the extent of lesions on the affected body parts, socioeconomic status and participation levels in social and domestic lives, was done. Results: The result showed that, 17.3% of vitiligo patients participated minimally in domestic and social life, whereas 28% of psoriasis patients had this problem (P=0.027. Extreme participation restriction was observed only among psoriasis patients (2.7%. Psoriasis patients also faced significantly more restrictions in a number of day-to-day life situations such as, less confidence in learning and applying knowledge, difficulties in meaningfully participating in major life areas like, work, education and employment, and also in community, social and civic life (all three domains P<0.0001, to vitiligo patients. Conclusion: Both psoriasis and vitiligo patients suffered moderate to severe restriction while participating in their domestic and social life. Of these two groups, psoriasis patients faced significantly more restrictions in a number of day-to-day life situations. The Indian population of this study was predominantly dark-skinned and hypo-pigmentation as seen in vitiligo is much more noticeable than psoriatic red patches. However, the results showed that the component of hypo or hyperpigmentation of the skin is not the only factor leading to participation restrictions.

  8. Increased frequency of self-reported parasomnias in patients suffering from vitiligo.

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    Mouzas, Odysseas; Angelopoulos, Nikiforos; Papaliagka, Maria; Tsogas, Panagiotis

    2008-01-01

    Vitiligo is a skin disorder of unknown aetiology, affecting 0.1-2% of the general population. The aim of the present study was to investigate its relationship with sleep disorders, especially parasomnias. Two hundred and sixteen individuals were examined. Among them, 116 were suffering from vitiligo, 52 from other dermatological diseases and 48 were healthy subjects, serving as a control group. An inventory including items related to sleep disorders from childhood and adolescence was used. The study was focused specifically on parasomnias. Patients suffering from vitiligo reported a significantly higher occurrence of sleepwalking, nocturnal enuresis, night illusions, sleep terrors and nightmares than that of the control group, prior to the manifestation of the disease. Patients suffering from other dermatological diseases only reported significantly more often nightmares and nocturnal enuresis compared to the control group. A relationship between parasomnias during early life and later development of vitiligo was detected. This finding supports the hypothesis that neural mechanisms involving monoaminergic systems (especially the serotoninergic one) may potentially be involved in the aetiopathology of vitiligo.

  9. Assessment of illness-related indicators in peripheral blood and skin lesion tissue of patients with vitiligo after NB-UVB, triamcinolone acetonide and vitiligo granules triple treatment

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    Wei-Liang Wang; Yan Chen; Feng-Bin Liu; Yuan-Zuo Huang; Ju-Zhen Lin; Guan-Biao Lyu

    2016-01-01

    Objective:To study the changes of illness-related indicators in peripheral blood and skin lesion tissue of patients with vitiligo after NB-UVB, triamcinolone acetonide and vitiligo granules triple treatment.Methods: Patients with vitiligo were selected as research subjects and randomly divided into two groups, observation group received NB-UVB, triamcinolone acetonide and vitiligo granules triple treatment, control group received combined therapy of NB-UVB and triamcinolone acetonide, and then the contents of lymphocyte subsets and cytokines in peripheral blood as well as the expression of illness-related molecules in skin lesion tissue were detected.Results:After treatment, the percentage of CD3+ CD28+, CD3+CD4+CD28+, CD3+CD8+CD28+ and Th17cells as well as the contents of IL-17 and IL-6 in peripheral blood of observation group were significantly lower than those of control group, and the percentage of CD3+ CTLA4+, CD3+CD4+ CTLA4+, CD3+CD8+ CTLA4+ and Treg cells as well as the contents of IL-10 and TGF-β were significantly higher than those of control group; the expression levels of Nrf-2, SCF, c-kit and InnVit in skin lesion tissue of observation group were higher than those of control group, and CLEC2B expression level was lower than that of control group.Conclusion:NB-UVB, triamcinolone acetonide and vitiligo granules triple treatment can more effectively regulate immune function and expression of illness-related molecules in skin lesion tissue of patients with vitiligo.

  10. SIRT1 regulates MAPK pathways in vitiligo skin: insight into the molecular pathways of cell survival.

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    Becatti, Matteo; Fiorillo, Claudia; Barygina, Victoria; Cecchi, Cristina; Lotti, Torello; Prignano, Francesca; Silvestro, Agrippino; Nassi, Paolo; Taddei, Niccolò

    2014-03-01

    Vitiligo is an acquired and progressive hypomelanotic disease that manifests as circumscribed depigmented patches on the skin. The aetiology of vitiligo remains unclear, but recent experimental data underline the interactions between melanocytes and other typical skin cells, particularly keratinocytes. Our previous results indicate that keratinocytes from perilesional skin show the features of damaged cells. Sirtuins (silent mating type information regulation 2 homolog) 1, well-known modulators of lifespan in many species, have a role in gene repression, metabolic control, apoptosis and cell survival, DNA repair, development, inflammation, neuroprotection and healthy ageing. In the literature there is no evidence for SIRT1 signalling in vitiligo and its possible involvement in disease progression. Here, biopsies were taken from the perilesional skin of 16 patients suffering from non-segmental vitiligo and SIRT1 signalling was investigated in these cells. For the first time, a new SIRT1/Akt, also known as Protein Kinase B (PKB)/mitogen-activated protein kinase (MAPK) signalling has been revealed in vitiligo. SIRT1 regulates MAPK pathway via Akt-apoptosis signal-regulating kinase-1 and down-regulates pro-apoptotic molecules, leading to decreased oxidative stress and apoptotic cell death in perilesional vitiligo keratinocytes. We therefore propose SIRT1 activation as a novel way of protecting perilesional vitiligo keratinocytes from damage.

  11. Comparison of plasma malondialdehyde, glutathione, glutathione peroxidase, hydroxyproline and selenium levels in patients with vitiligo and healthy controls

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    Ozturk I

    2008-01-01

    Full Text Available Background: The etiology and pathophysiologic mechanism of vitiligo are still unclear. The relationship between increased oxidative stress due to the accumulation of radicals and reactive oxygen species and the associated changes in blood and epidermal component of vitiliginous skin have been reported many times. We investigated the possible changes of plasma malondialdehyde, glutathione, selenium, hydroxyproline and glutathione peroxidase activity levels in patients with vitiligo in order to evaluate the relationship between oxidative stress and etiopathogenesis of vitiligo. Materials and Methods: Plasma malondialdehyde, glutathione, hydroxyproline and glutathione peroxidase activity levels were measured by spectrophotometric methods, and HPLC was used for measurement of selenium concentrations. Results: Our results showed increased malondialdehyde, hydroxyproline and glutathione peroxidase activity levels in plasma of vitiligo group ( P < 0.05. Conclusion: Support of antioxidant system via nonenzymatic antioxidant compounds and antioxidant enzymes may be useful to prevent of melanocyte degeneration which occur due to oxidative damage in vitiligo.

  12. On the pathophysiology of vitiligo: Possible treatment options

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    Raymond E Boissy

    2012-01-01

    Full Text Available Vitiligo is an acquired depigmenting disorder usually classified as non-segmental and segmental types with a higher incidence of the non-segmental ones. The cause of non-segmental vitiligo is still unknown. Currently, it is a dogma that there are several genes affecting the immune system and the pigment system that predisposes someone to develop vitiligo. A precipitating factor must then ellicit an interaction between the immune system and the melanocyte, resulting in destruction of the melanocyte population in discrete areas of the skin. Starting from the overlapping but distinct pathomechanisms, treatment should be finalized to the cellular targets and possibly related to the disease phase.

  13. On the pathophysiology of vitiligo: possible treatment options.

    Science.gov (United States)

    Boissy, Raymond E; Dell'Anna, Maria Lucia; Picardo, Mauro

    2012-01-01

    Vitiligo is an acquired depigmenting disorder usually classified as non-segmental and segmental types with a higher incidence of the non-segmental ones. The cause of non-segmental vitiligo is still unknown. Currently, it is a dogma that there are several genes affecting the immune system and the pigment system that predisposes someone to develop vitiligo. A precipitating factor must then ellicit an interaction between the immune system and the melanocyte, resulting in destruction of the melanocyte population in discrete areas of the skin. Starting from the overlapping but distinct pathomechanisms, treatment should be finalized to the cellular targets and possibly related to the disease phase.

  14. Vitiligo associated with esophageal adenocarcinoma

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    Ali Asilian

    2013-01-01

    Full Text Available Vitiligo is a disease that results in depigmented areas in the skin. It may develop at any age but the average age at onset is 20 years. Association of vitiligo and melanoma has been commonly reported, but malignancies other than melanoma have been rarely associated with vitiligo. We report a 73-year-old patient with new onset vitiligo who developed esophageal adenocarcinoma in the following years.

  15. Association of Angiotensin-Converting Enzyme (ACE Gene Polymorphism with Inflammation and Cellular Cytotoxicity in Vitiligo Patients.

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    Laila Rashed

    Full Text Available Vitiligo is a disorder with profound heterogeneity in its aetio-pathophysiology. Angiotensin converting enzyme (ACE plays an important role in the physiology of the vasculature, blood pressure and inflammation. An insertion/deletion (I/D polymorphism of the ACE gene was reported be associated with the development of vitiligo.Our aim was to evaluate the ACE I/D polymorphism in vitiligo patients and controls. Our second aim was to find a possible association between ACE gene polymorphism and inflammatory mediators (as interleukin (IL-6 and/or cellular cytotoxicity induced by serum nitrite (as a breakdown product of the cytotoxic nitric oxide in vitiligo patients.This case-control study included 74 vitiligo patients and 75 apparently healthy controls. The distribution of ACE gene I/D genotype was investigated using PCR. Serum ACE, IL-6 and nitrite were measured by colorimetric method, ELISA and Griess assay respectively.The ACE allele frequency was significantly different between vitiligo patients and healthy controls (P = 0.026. However there was no significant difference between the ACE genotyping frequency in both groups (P = 0.115. There were statistically significant higher VIDA score (P = 0.007, and serum IL-6 (P < 0.001 in patients with the DD genotype when compared to other genotypes. Serum nitrite in patients with the DD genotype was significantly higher (P = 0.007 when compared to patients with II genotype. Serum levels of ACE, IL-6 and nitrite in vitiligo patients were statistically significantly higher than those in controls.As a conclusion, ACE gene polymorphism might grant susceptibility to develop vitiligo. Serum IL-6 and nitrite levels might have an important role in the pathogenesis of vitiligo. Targeting these two factors might have an implication in the treatment of some resistant cases.

  16. Increased prevalence of thyroid dysfunction and diabetes mellitus in Indian vitiligo patients: A case-control study

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    K.V.T. Gopal

    2014-01-01

    Full Text Available Background: Though it is well-known that vitiligo is associated with other autoimmune disorders, few Indian studies have focused on the relation between vitiligo, autoimmune thyroid dysfunction and diabetes mellitus. Materials and Methods: This case-control study included 150 new cases of vitiligo and 100 age and sex-matched controls. A complete history and thorough dermatological examination was done. Serum samples from both patients and controls were collected and assayed for triiodothyronine, thyroxine, thyroid-stimulating hormone, anti-thyroid antibodies-anti-thyroid peroxidase and anti-thyroglobulin and fasting plasma glucose. Results: Thyroid hormonal profile revealed autoimmune thyroid dysfunction manifesting as hypothyroidism in 30 (20% vitiligo patients and two controls (2%. Diabetes mellitus was present in 24 (16% vitiligo patients and five controls. Seven (4.7% patients had both hypothyroidism and diabetes mellitus. Conclusion: There is a clear association between vitiligo, autoimmune hypothyroidism and diabetes mellitus. It would be very useful to screen for thyroid dysfunction and diabetes mellitus in all patients with vitiligo.

  17. Quality of life in vitiligo patients after treatment with long-term narrowband ultraviolet B phototherapy.

    NARCIS (Netherlands)

    Tjioe, M.; Otero, M.; Kerkhof, P.C.M. van de; Gerritsen, M.J.P.

    2005-01-01

    Long-term treatments for chronic diseases such as vitiligo need to be evaluated for their clinical efficacy. Assessment of the quality of life (QOL), however, may provide the most relevant information on the actual benefit for these patients. In this study we evaluated QOL after long-term narrowband

  18. A comparative and observational assessment of gingival condition and il-1β level in vitiligo and nonvitiligo patients

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    Kharidhi Laxman Vandana

    2016-01-01

    Full Text Available Introduction: Vitiligo is an autoimmune skin disorder that causes loss of skin pigment. Loss of skin pigment in oral mucosa may also become prominent and tend to be more noticeable in people with darker skin. There is much less information concerning this condition in gingiva. Therefore, an initial attempt has been made to compare clinical gingival status and biochemical assessment in vitiligo and nonvitiligo patients. Materials and Methods: This observational pilot study includes 45 patients of age group 20–40 years were divided as nonvitiligo pigmented gingivitis as observational 1 group, nonvitiligo nonpigmented gingivitis as control group, and vitiligo with gingivitis as observational 2 group. The clinical parameters assessed were plaque index, gingival index (GI, gingival bleeding index (GBI, dummett oral pigmentation index, and biochemical parameter such as gingival crevicular fluid (GCF interleukin-1 beta (IL-1β levels. Results: On intergroup examination using unpaired t -test and statistical analysis, vitiligo group showed highly significant results in GI (P < 0.001 and GBI (P < 0.001 than nonvitiligo group. Furthermore, the GCF IL-1β levels were found to be lesser (34.16 ng/ml in vitiligo group as compared to nonvitiligo groups. Conclusion: At similar plaque level, higher gingival inflammation and bleeding were found in vitiligo patients. The loss of pigmentation of gingiva was evident in vitiligo patients. The possible defensive role of melanin is evident clinically.

  19. Survey and online discussion groups to develop a patient-rated outcome measure on acceptability of treatment response in vitiligo.

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    Tour, Selina K; Thomas, Kim S; Walker, Dawn-Marie; Leighton, Paul; Yong, Adrian Sw; Batchelor, Jonathan M

    2014-06-14

    Vitiligo is a chronic depigmenting skin disorder which affects around 0.5-1% of the world's population. The outcome measures used most commonly in trials to judge treatment success focus on repigmentation. Patient-reported outcome measures of treatment success are rarely used, although recommendations have been made for their inclusion in vitiligo trials. This study aimed to evaluate the face validity of a new patient-reported outcome measure of treatment response, for use in future trials and clinical practice. An online survey to gather initial views on what constitutes treatment success for people with vitiligo or their parents/carers, followed by online discussion groups with patients to reach consensus on what constitutes treatment success for individuals with vitiligo, and how this can be assessed in the context of trials. Participants were recruited from an existing database of vitiligo patients and through posts on the social network sites Facebook and Twitter. A total of 202 survey responses were received, of which 37 were excluded and 165 analysed. Three main themes emerged as important in assessing treatment response: a) the match between vitiligo and normal skin (how well it blends in); b) how noticeable the vitiligo is and c) a reduction in the size of the white patches. The majority of respondents said they would consider 80% or more repigmentation to be a worthwhile treatment response after 9 months of treatment. Three online discussion groups involving 12 participants led to consensus that treatment success is best measured by asking patients how noticeable their vitiligo is after treatment. This was judged to be best answered using a 5-point Likert scale, on which a score of 4 or 5 represents treatment success. This study represents the first step in developing a patient reported measure of treatment success in vitiligo trials. Further work is now needed to assess its construct validity and responsiveness to change.

  20. Assessment of vitamin D plasma levels in patients with vitiligo vulgaris.

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    Akram Beheshti

    2014-08-01

    Full Text Available Vitiligo Vulgaris is a pigment disorder as the lack of skin pigmentation which its most prevalent cause is autoimmune. Vitamin D has various effects on the natural and acquired immune of the body because of its effectiveness on the T cells and B cells, macrophages and dendritic cells.The object of this study was assessment of the Vitamin D in patients with vitiligo vulgaris in dermatologic clinics in Qazvin in 2012. The 100 patients with Vitiligo were studied through a cross-sectional study. The required data were collected by the questionnaire (age, gender, job, family background, consumption of the dairies and vitamin D supplements, examination (type of skin and para clinical test (measurement of the serum 25-hydroxyvitamin D level. The 42 (42% and 58 (58% people of the population under study were respectively male and female. The mean age of the population under study was 28.7±1.17. The mean serum of 25-hydroxyvitamin D level was 42±24.14 which had a significance difference with a normal level (p<0.04. The mean serum 25-hydroxyvitamin D level among patients with Vitiligo had a significance difference with a normal level.

  1. A study of the free radical scavenging effects of Piper betle leaf extract in patients with vitiligo

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    Sneha Mitra

    2017-01-01

    Full Text Available Background: Vitiligo is an idiopathic skin disease manifested by depigmented macules. It is characterised by melanocyte destruction, and redox imbalance is proposed to play a contributory role. Aim: The aim of this study was to analyze the effects of an ethanolic extract of Piper betle leaves on the generation of reactive oxygen species in erythrocytes sourced from vitiligo patients. Methods: The effect of Piper betle on the generation of reactive oxygen species in erythrocytes was measured by flow cytometry in patients with active and stable vitiligo versus healthy controls, using 5-(and-6-chloromethyl-2'-7'-dichlorodihydrofluorescein diacetate. Results: The generation of reactive oxygen species in erythrocytes was higher in patients with vitiligo (n = 23 compared to healthy controls (n = 18. The geometrical mean fluorescence channel was 23.05 ± 2.11 in patients versus 17.77 ± 1.79 in controls, P = 0.039. The levels of reactive oxygen species were higher in patients with active vitiligo. Treatment of erythrocytes with Piper betle in concentrations of 0.5 and 1.0 μg/ml significantly decreased the baseline levels of reactive oxygen species by 31.7% in healthy controls, and 47.6% and 44.3% in patients with active vitiligo, respectively. Piper betle effectively scavenged hydrogen peroxide, which was evident by a decrease in the geometrical mean fluorescence channel by 52.4% and 62.9% in healthy controls, and 45.0% and 57.0% in patients with active vitiligo. Limitations: The study had a small sample size. Future studies should focus on evaluation of the antioxidant role of Piper betle at the lesional site. Conclusion: This pilot study indicates that patients with active vitiligo demonstrate enhanced generation of reactive oxygen species in erythrocytes, which was significantly reduced following ex vivo treatment with Piper betle.

  2. A study of the free radical scavenging effects of Piper betle leaf extract in patients with vitiligo.

    Science.gov (United States)

    Mitra, Sneha; Pati, Ayan Kumar; Manna, Alak; Ghosh, Arghyaprasun; Sen, Sumit; Chatterjee, Suparna; Chatterjee, Mitali

    2017-01-01

    Vitiligo is an idiopathic skin disease manifested by depigmented macules. It is characterised by melanocyte destruction, and redox imbalance is proposed to play a contributory role. The aim of this study was to analyze the effects of an ethanolic extract of Piper betle leaves on the generation of reactive oxygen species in erythrocytes sourced from vitiligo patients. The effect of Piper betle on the generation of reactive oxygen species in erythrocytes was measured by flow cytometry in patients with active and stable vitiligo versus healthy controls, using 5-(and-6)-chloromethyl-2'-7'-dichlorodihydrofluorescein diacetate. The generation of reactive oxygen species in erythrocytes was higher in patients with vitiligo (n = 23) compared to healthy controls (n = 18). The geometrical mean fluorescence channel was 23.05 ± 2.11 in patients versus 17.77 ± 1.79 in controls, P = 0.039. The levels of reactive oxygen species were higher in patients with active vitiligo. Treatment of erythrocytes with Piper betle in concentrations of 0.5 and 1.0 μg/ml significantly decreased the baseline levels of reactive oxygen species by 31.7% in healthy controls, and 47.6% and 44.3% in patients with active vitiligo, respectively. Piper betle effectively scavenged hydrogen peroxide, which was evident by a decrease in the geometrical mean fluorescence channel by 52.4% and 62.9% in healthy controls, and 45.0% and 57.0% in patients with active vitiligo. The study had a small sample size. Future studies should focus on evaluation of the antioxidant role of Piper betle at the lesional site. This pilot study indicates that patients with active vitiligo demonstrate enhanced generation of reactive oxygen species in erythrocytes, which was significantly reduced following ex vivo treatment with Piper betle.

  3. Association of vitiligo with anemia, vitamin B12 deficiency, diabetes mellitus, and thyroid dysfunction in Saudi Arab patients: A case control study

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    Thamer Mubki

    2017-07-01

    Full Text Available Objectives: To assess the association of vitiligo with thyroid dysfunction, vitamin B12 deficiency, diabetes mellitus and anemia; among Saudi Arab individuals using laboratory data in a controlled fashion. Methods: A case control study was conducted among Saudi nationals with a confirmed diagnosis of vitiligo presenting between July 2014 and December 2015. Sex- and age-matched randomly selected subjects were included as controls. Blood samples from both vitiligo patients and controls were collected and assayed for hemoglobin level, mean corpuscular volume, thyroid stimulating hormone level, free thyroxine level, vitamin B12, and fasting blood glucose. Results: Of the 115 vitiligo subjects, 15% had microcytic anemia compared to 7% of control (p = 0.072. Macrocytic anemia was diagnosed in 1% of vitiligo subjects and in 2% of control (p = 0.404. Vitiligo group had significantly higher prevalence (5% of primary hypothyroidism compared to control (0% (p = 0.030. Vitamin B12 deficiency was significantly more prevalent in vitiligo group (16% compared to control (2% (p = 0.001. The prevalence of diabetes mellitus was higher in vitiligo group (8% as compared to control (4% (p = 0.334. Conclusion: Primary hypothyroidism and vitamin B12 deficiency are significantly more prevalent in Saudi Arab vitiligo patients. Screening vitiligo patients for thyroid dysfunction and vitamin B12 deficiency may be warranted.

  4. Female Genital Dialogues: Female Genital Self-Image, Sexual Dysfunction, and Quality of Life in Patients With Vitiligo With and Without Genital Affection.

    Science.gov (United States)

    Sarhan, Deena; Mohammed, Ghada F A; Gomaa, Amal H A; Eyada, Moustafa M K

    2016-01-01

    Vitiligo has a major effect on sexual health because of the disfiguring skin lesions affecting self-image and self-esteem. However, this topic has not explored. This article aimed to assess the effect of vitiligo on genital self-image, sexual function, and quality of life in female patients. This cross-sectional study included 50 sexually active women with vitiligo and 25 women without vitiligo. All participants subjected to full history taking and examination. Extent of vitiligo was assessed with the Vitiligo Area Scoring Index score, sexual function with the Female Sexual Function Index, genital self-image with Female Genital Self-Image Score and quality of life with the Dermatology Life Quality Index questionnaires. The main outcome measures were correlation between Vitiligo Area Scoring Index, Female Genital Self-Image Score, Female Sexual Function Index, and Dermatology Life Quality Index domains was determined using t test and Pearson correlation. This study revealed a negative correlation between the Vitiligo Area Scoring Index score and sexual satisfaction. Vitiligo Area Scoring Index and Dermatology Life Quality Index score was significantly correlated with Arabic Version of the Female Genital Self-Image Score alone and with Arabic Version of the Female Sexual Functioning Index alone and with both the Arabic Version of the Female Genital Self-Image Score and the Arabic Version of the Female Sexual Functioning Index (p vitiligo is imperative to improve outcomes and increase patients' compliance with treatment.

  5. Study of oxidative stress in peripheral blood of Indian vitiligo patients

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    Sudha S Deo

    2013-01-01

    Full Text Available Background: Vitiligo is an acquired skin disease that involves the interplay of complex genetic, immunological, neural and self-destructive mechanisms in its pathogenesis. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. Objectives: The aim of our investigation was to evaluate the role of oxidative stress by studying the role of catalase (CAT in the destruction of melanocytes in patients with vitiligo and compare the same in healthy normal controls. Materials and Methods: We determined the serum catalase enzyme by ELISA method. The catalase activity was studied in two groups, Group I-localized vitiligo: (i active stage, (ii static or inactive stage and Group II-generalized vitiligo: (i active stage, (ii static or inactive stage patients, and the levels were compared with healthy controls. Results: Group I active stage patients showed significant difference in the catalase levels with a P < 0.044 when compared with healthy controls, whereas Group II static stage patients did not show any significant difference (P < 0.095 although the catalase activity was increased. Conclusion: Our study could not explain the cause of melanocyte damage in patients in the active stage of the disease. The increase in the oxidative stress as detected by catalase activity was more significant in Group I active disease than Group II active disease patients although the levels were higher than the healthy normals. This is the first study conducted on active and static stage of vitilgo in India. It is possible that the number of compounds of hydrogen peroxide produced is not balanced by the production of catalase in the body.

  6. Vitiligo in Children: A Birds Eye View.

    Science.gov (United States)

    Phiske, Meghana M

    2016-01-01

    Vitiligo in children is a distinct subset of vitiligo and differs from adult vitiligo. Characteristic features include family history of autoimmune or endocrine disease, higher incidence of segmental vitiligo, development of early or premature graying, increased incidence of autoantibodies and poor response to topical PUVA. The exact prevalence of vitiligo in children varies between 0.1-4% of the world population and seems to be higher in India than in other countries and it occurs more frequently in females. Around 12% to 35% of pediatric vitiligo patients have family members with the disease. The most common type of vitiligo in pediatric patients is vitiligo vulgaris, representing 78% of cases. The most commonly associated autoimmune disease is thyroiditis. Phototherapy and topical corticosteroids are the most commonly used treatments for adult vitiligo but are less useful in the pediatric population.

  7. Epitopes, avidity and IgG subclasses of tyrosine hydroxylase autoantibodies in vitiligo and alopecia areata patients.

    Science.gov (United States)

    Rahoma, S F E; Sandhu, H K; McDonagh, A J G; Gawkrodger, D J; Weetman, A P; Kemp, E H

    2012-07-01

    We previously detected antibodies against tyrosine hydroxylase (TH) in 23% of patients with nonsegmental vitiligo and in 19% of patients with alopecia areata (AA). To identify TH epitopes recognized by TH antibodies in patients with vitiligo and AA. Recombinant plasmids containing defined fragments of TH cDNA were constructed. The cloned TH cDNA fragments were subsequently translated in vitro to produce a series of [(35) S]-labelled TH protein fragments which were then used in radioimmunoassays to analyse the immunoreactivity of sera from 18 TH antibody-positive patients with vitiligo and so initially define TH epitope domains. Further localization of TH epitopes was investigated by antibody absorption experiments using synthetic TH peptides and nonradiolabelled, in vitro-expressed TH protein fragments. Antibody binding to identified epitopes was confirmed in TH peptide enzyme-linked immunosorbent assays. Analysis of the results obtained indicated the presence of two major antibody-binding sites on TH between amino acids 1 and 14 (epitope 1-14) and between amino acids 61 and 80 (epitope 61-80). Of 18 patients with vitiligo and six with AA, 17 (94%) and five (83%), respectively, had antibodies against epitope 1-14. In addition, 11/18 (61%) vitiligo and 2/6 (33%) AA patient sera displayed immunoreactivity against epitope 61-80. Two major binding sites for human TH antibodies are located at the N-terminus of the protein. The humoral immune response to TH in vitiligo and AA is heterogeneous in nature in that patients may have antibodies to more than one TH epitope. TH antibodies from patients with vitiligo or AA can recognize identical epitopes. © 2012 The Authors. BJD © 2012 British Association of Dermatologists.

  8. Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity.

    Science.gov (United States)

    Strassner, James P; Rashighi, Mehdi; Ahmed Refat, Maggi; Richmond, Jillian M; Harris, John E

    2017-05-01

    Vitiligo is an autoimmune disease of the skin with limited treatment options; there is an urgent need to identify and validate biomarkers of disease activity to support vitiligo clinical studies. To investigate potential biomarkers of disease activity directly in the skin of vitiligo subjects and healthy subjects. Patient skin was sampled via a modified suction-blister technique, allowing for minimally invasive, objective assessment of cytokines and T-cell infiltrates in the interstitial skin fluid. Potential biomarkers were first defined and later validated in separate study groups. In screening and validation, CD8(+) T-cell number and C-X-C motif chemokine ligand (CXCL) 9 protein concentration were significantly elevated in active lesional compared to nonlesional skin. CXCL9 protein concentration achieved greater sensitivity and specificity by receiver operating characteristic analysis. Suction blistering also allowed for phenotyping of the T-cell infiltrate, which overwhelmingly expresses C-X-C motif chemokine receptor 3. A small number of patients were enrolled for the study, and only a single patient was used to define the treatment response. Measuring CXCL9 directly in the skin might be effective in clinical trials as an early marker of treatment response. Additionally, use of the modified suction-blister technique supports investigation of inflammatory skin diseases using powerful tools like flow cytometry and protein quantification. Copyright © 2016 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.

  9. Effect of an antioxydant cream versus placebo in patients with vitiligo in association with excimer laser. A pilot randomized, investigator-blinded, and half-side comparison trial.

    Science.gov (United States)

    Leone, G; Paro Vidolin, A

    2015-08-01

    The aim of this study was to evaluate the combined effect of excimer laser and a topical antioxidant in the treatment for vitiligo. The study was conducted in a single blinded design on 10 vitiligo patients with symmetrical vitiligo lesions treated with the active antioxidant or a placebo that were irradiated with an excimer laser. Results have shown that the lesions treated with the active cream achieved earlier regimentation compared to the placebo. The use of a cream containing antioxidants may improve the results of excimer laser treatment in patients with vitiligo.

  10. Quality of life and psychological morbidity in vitiligo patients: A study in a teaching hospital from north-east India

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    Lucybeth N Sangma

    2015-01-01

    Full Text Available Background : Vitiligo is an acquired discoloration of skin and mucous membrane of great cosmetic importance affecting 1-4% of the world′s population. It causes disfiguration in all races, more so in dark-skinned people because of strong contrast. Men, women, and children with vitiligo face severe psychological and social disadvantage. Aim: To assess the impact of the disease on the quality of life of patients suffering from vitiligo, also to ascertain any psychological morbidity like depression associated with the disease and to compare the results with that of healthy control group. Materials and Methods : Dermatology Life Quality Index (DLQI and Hamilton Depression Rating Scale (HAMD are administered to 100 vitiligo patients presenting to the Dermatology OPD and 50 age- and sex-matched healthy controls. Results were analyzed and compared with that of control group. Findings are also correlated in relation to demographic and clinical profile of the disease. Statistical analysis is made to see the significance. Results : Vitiligo-affected patients had significantly elevated total DLQI scores (P < 0.001 compared to healthy controls. There is increase in parameters like itch, embarrassment, social and leisure activities in the patient cohort than the control group. Patients of vitiligo are also found to be more depressed (P < 0.001 than the controls. Conclusion : Quality of life (QOL in patients affected with vitiligo declined more severely, and also there is increase in incidence of depression than in the control group. These changes are critical for the psychosocial life of the affected people.

  11. Detection of serum anti-melanocyte antibodies and identification of related antigens in patients with vitiligo.

    Science.gov (United States)

    Zhu, M C; Liu, C G; Wang, D X; Zhan, Z

    2015-12-07

    We detected autoantibodies against melanocytes in serum samples obtained from 50 patients, including 4 with HBV, with vitiligo and identified the associated membrane antigens. Heat shock protein 70 (HSP70) and anti-tyrosinase-related protein 1 (TRP-1) antibody levels were analyzed. The associated antigens in normal human melanocyte were identified by immunofluorescence. Autoantibodies against melanocyte membrane and cytoplasmic proteins were detected by western blot. Membrane antigens with higher frequencies were identified by protein mass spectrometry. The HSP70 and anti-TRP-1 antibody levels (N = 70; 10 with HBV) were detected by ELISA. The specific antigens were detected in melanocyte cytoplasm and membrane (40/50; 80% incidence; western blot). The autoantibodies reacted with several membrane antigens with approximate molecular weights (Mr) of 86,000, 75,000, 60,000, 52,000, and 44,000 (strip positive rates: 36, 58, 22, 2, and 2%, respectively). Thirty percent of the patients showed the presence of cytoplasmic antigens (Mr: 110,000, 90,000, 75,000, 50,000, and 400,000; strip positive rates: 12, 4, 12, 10, and 2%, respectively). Fifteen and 5% of the healthy subjects showed positive expression of membrane and cytoplasmic antigens, respectively. Protein mass spectrometry predicted membrane proteins with Mr of 86,000 and 75,000 and 60,000 to be Lamin A /C and Vimentin X1, respective. High titers of anti-TRP-1 antibody were detected and showed positive correlation with HSP70 (r = 0. 927, P vitiligo, which might assist future investigations into autoimmune pathogenesis of vitiligo and formation of autoantibodies. HBV infection was correlated to vitiligo.

  12. The association of FOXO3A gene polymorphisms with serum FOXO3A levels and oxidative stress markers in vitiligo patients.

    Science.gov (United States)

    Ozel Turkcu, Ummuhani; Solak Tekin, Nilgun; Gokdogan Edgunlu, Tuba; Karakas Celik, Sevim; Oner, Setenay

    2014-02-15

    Vitiligo is an acquired epidermal pigment loss of the skin. Oxidative stress is one of the major theories in the pathophysiology of vitiligo. FOXO3A is the forkhead members of the class O (FOXO) transcription factors, and plays an important role in cell cycle regulation, apoptosis, oxidative stress, and DNA repair. The aim of our study was to investigate FOXO3A gene polymorphisms and FOXO3A protein levels, activities of superoxide dismutase (SOD) and catalase antioxidant enzymes in vitiligo patients and healthy controls. Moreover, the level of plasma advanced oxidation protein products (AOPP) in subjects was evaluated to understand the possible role of protein oxidation in disease etiology. Study groups included 82 vitiligo patients and 81 unrelated healthy controls. FOXO3A polymorphisms were determined using polymerase chain reaction-restriction fragment length polymorphism method. FOXO3A levels and catalase activity were measured by ELISA whereas AOPP levels and SOD activity was measured by spectrophotometric analysis. We found a significant relationship between rs4946936 polymorphism of FOXO3A gene and vitiligo/active vitiligo patients (p=0.017; p=0.019 respectively), but not for rs2253310 (p>0.05). SOD activity and AOPP levels of vitiligo patient were increased compared with control group, whereas FOXO3A levels and catalase enzyme activity of vitiligo patient were decreased compared with control group (pvitiligo, especially active vitiligo. Moreover, our results confirm that oxidative stress may play a role in the pathophysiology of vitiligo. Further studies with larger samples are required to elucidate the role of FOXO3A in vitiligo.

  13. Vitiligo- A surgical approach

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    Deepti Ghia

    2012-01-01

    Full Text Available Aims and objective- To describe the spectrum of surgical modalities for stable vitiligo patients Methods- Patients having stable vitiligo since past 2 years with no improvement with medical line of treatment were enrolled for surgery after informed consent. Depending upon the size and location of vitiligo patch different modalities were performed. Suction blister, mini-punch grafting, split thickness skin grafting, trypsinised melanocyte-keratinocyte transfer and non-trypsinised melanocyte- keratinocyte transfer (Jodhpur technique and follicular grafting technique have been described photographically which have been performed at a tertiary care hospital. Conclusion- Vitiligo is often difficult to treat, stable patches resistant to medical line of management do respond to surgical treatment; however it is very important to choose the modality of surgery according location of the patch, size of the lesion and available resources.

  14. Radio-active iodine uptake in vitiligo

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    Kumar, V.; Shankar, V.; Chaudhary, S.; Bhatia, K.K.; Mehta, L.K.; Arora, D.R. (Medical College and Hospital, Rohtak-124001 (India))

    1990-01-01

    Vitiligo and thyroid disease are commonly associated disorders. Twenty-two clinically euthyroid vitiligo patients were studied for functional assessment of thyroid by radioactive iodine uptake assay. Half of them showed abnormal uptake values at 24 hours. Of these patients, 90% had lower values indicating a tendency towards developing hypothyroid state. Subclinical thyroid dysfunction in vitiligo appears to be an adaptive change. (author).

  15. Risk factors and treatment responses in patients with vitiligo in Japan--A retrospective large-scale study.

    Science.gov (United States)

    Ohguchi, Ryoko; Kato, Hiroshi; Furuhashi, Takuya; Nakamura, Motoki; Nishida, Emi; Watanabe, Shoichi; Shintani, Yoichi; Morita, Akimichi

    2015-05-01

    Vitiligo is a refractory skin disease. To investigate the risk factors and treatment responses of patients with vitiligo in Japan, we recorded and analyzed the details of 713 vitiligo patients (comorbidity, treatment responses, family history, age, and sex) who visited the dermatology clinic of the Nagoya City University Hospital, Nagoya, Japan between January 2004 and August 2010 (mean age, 35.2 years; 302 men, 411 women) using logistic regression analysis. The results are expressed as odds ratios (OR) with 95% confidence interval (CI). Patients were diagnosed with vitiligo [n = 644; 338 generalized type (47.4%), 170 segmental type (23.8%), and 136 localized type (19.1%)], nevus depigmentosus (n = 53, 7.4%), halo nevus (n = 14, 2.0%), and hypomelanosis of Ito (n = 2, 0.3%). For generalized and localized types, none of the analyzed factors were statistically significant. For the segmental type, antinuclear antibody (OR = 1.005; 95% CI, 1.00-1.01; p 55 years (OR = 0.0171; 95% CI, 0.00333-0.0879; p vitiligo.

  16. The Efficacy Of Low-Dose Oral Corticosteroids In The Treatment Of Vitiligo Patients

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    Mirshams-Shahshahani M; Halaji Z; Ehsani AH; Toosi S

    2005-01-01

    Background: Vitiligo is an acquired pigmentary disorder that affects 1% of population. It presents as depigmented patches. One of the most probable theories regarding the pathogenesis of vitiligo is autoimmunity. Systemic corticosteroids may arrest the progression of vitiligo and lead to repigmentation by suppressing immune system. The objective of this study is to assess the clinical efficacy of low-dose oral corticosteroids in actively progressing vitiligo. Materials and Methods: Seventy fo...

  17. Assessment of MC1R and α-MSH gene sequences in Iranian vitiligo patients

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    Eskandani M

    2010-01-01

    Full Text Available Background: Vitiligo is an acquired pigmentary disorder of the skin that is caused by unknown factors and is characterized by white and depigmented patches that enlarge and become more numerous with time. Genetic factors, oxidative stress, autoimmunity, and neurochemical agents, such as catecholamines might also contribute to vitiligo. Cutaneous pigmentation is determined by the amounts of eumelanin and pheomelanin synthesized by the epidermal melanocytes and interference of melanocortin-1 receptor (MC1R, a G-protein coupled receptor, its normal agonist, alpha-melanocyte stimulating hormone (α-MSH, and key enzymes, such as tyrosinase, to protect against sun-induced DNA damage. The MC1R, a 7 pass trans-membrane G-protein coupled receptor, is a key control point in melanogenesis. Loss-of-function mutations at the MC1R are associated with a switch from eumelanin to pheomelanin production, resulting in a red or yellow coat color. Aim: In this research, we aim to examine the genetic variety of MC1R and α-MSH gene in 20 Iranian vitiligo patients and 20 healthy controls. Materials and Methods: Analysis of the MC1R coding gene was performed with direct sequencing. Results: We found the following 9 MC1R coding region variants: Arg163Gl (G488A, Arg227Leu (G680A, Val 97Phe (G289T, Asp184Asn (G550A, Arg227Lys (G680A, Arg142His (G425A, Val60Leu (G178T, Val247Met (C739A, and Val174Ile (G520A. We also found 2 frameshift changes: one of them was the Insertion of C (frameshift in Pro136, stop at Trp148 and the other, Insertion of G (frameshift in Pro256, stop at Trp 333. Of all the changes, the most common was Val60Leu at 5% in patients vs 20% in controls, Val247Met at 15% in patients vs 0% in controls and Val174Ile at 15% in controls and 0% in patients. The other variants showed a frequency <5% in both patients and controls. Also in this study, we have examined the frequency of single nucleotide polymorphisms within the α-MSH genes with direct sequencing in

  18. Assessment and therapy of vitiligo

    NARCIS (Netherlands)

    Komen, L.

    2016-01-01

    Vitiligo is a common skin disorder causing depigmented macules that can alter a patient's appearance and impair a patient's quality of life. This thesis contains studies on different methods of assessment of vitiligo and their quality as well as studies assessing repigmentation and depigmentation th

  19. Contact vitiligo: etiology and treatment

    Directory of Open Access Journals (Sweden)

    Singh P

    2003-01-01

    Full Text Available Fifty patients of contact vitiligo were studied. EtiologicaI agents of contact vitiligo were identified by clinical history, distribution of lesions and patch testing with suspected material. All patients were advised to avoid the suspected agent and treated with PUVASOL and topical steroid. Out of 50 patients (Male 8%, Female 92% age 14-60 years., etiological agent of contact vitiligo was found to be sticking bindi alone in 24 (48%, while bindi along with other etiological agents were found to be purse, foot wear, plastic watch strap, lipstick and tooth paste in 14 ( 28% cases. 14 (28% patients also had disseminated lesions of vitiligo along with contact vitiligo. Positive reaction with patch testing was observed in 18 (36% while depigmentation was seen in 4 ( 8% cases. We observed that response of treatment was better in patients with shorter duration of disease while poor response was seen in patients with longer duration of disease.

  20. Photoletter to the editor: Dermatitis herpetiformis co-localised with vitiligo in a patient with autoimmune polyglandular syndrome.

    Science.gov (United States)

    Macbeth, Abby E; Lee, Kevin Y C; Levell, Nick J; Igali, Laszlo; Millington, George W M

    2013-01-01

    We report a case of dermatitis herpetiformis co-localised with segmental vitiligo in a 37-year-old woman with a background history of autoimmune polyglandular syndrome type 2. We propose genetic mosaicism as a possible mechanism. There has only been one previous case report in which dermatitis hepetiformis co-localised in close proximity but not exclusively within vilitigo in a patient with autoimmune thyroiditis. To our knowledge, this is the first case report of dermatitis herpetiformis co-localised exclusively to segmental vitiligo in the presence of autoimmune polyglandular syndrome.

  1. Vitiligo associated with cutaneous amyloidosis

    Directory of Open Access Journals (Sweden)

    Rajkumar V

    2001-09-01

    Full Text Available Vitiligo is known to be associated with a variety of dermatoses and systemic diseases. We describe a case of vitiligo developing in a patient having cutaneous amyloidosis. To our knowledge this is the first report of its kind in the literature.

  2. Insight into Natural History of Congenital Vitiligo: A Case Report of a 23-Year-Old with Stable Congenital Vitiligo

    Directory of Open Access Journals (Sweden)

    Chelsea Casey

    2017-01-01

    Full Text Available Vitiligo is a disorder of skin pigmentation. It affects approximately 1% of the world’s population. Vitiligo occurs equally between the sexes with no racial predilections. The majority of cases are acquired and arise between the second and third decades of life. Acquired vitiligo has an unpredictable clinical course. Congenital vitiligo is rare with few reported cases. Due to the rarity of congenital vitiligo, little is known about the clinical course. For patients with acquired or congenital vitiligo, the psychosocial burden can have a profound impact on quality of life. The unknown course of congenital vitiligo can exacerbate the feelings of distress and embarrassment. We report of a case of congenital vitiligo that has been stable for 23 years. The patient had no associated autoimmune disease. The pathogenesis of congenital vitiligo is unknown. This case may be useful to assist clinicians caring for newborns with congenital vitiligo in reassuring parents.

  3. Catalase -262C>T polymorphisms in Hungarian vitiligo patients and in controls: further acatalasemia mutations in Hungary.

    Science.gov (United States)

    Kósa, Zsuzsanna; Fejes, Zsolt; Nagy, Teréz; Csordás, Melinda; Simics, Enikő; Remenyik, Eva; Góth, László

    2012-04-01

    Catalase is the main regulator of hydrogen peroxide metabolism. In vitiligo patients there are conflicting data on its activity and no data on the effect of -262C>T polymorphism in the catalase gene. Blood catalase activity, -262C>T polymorphism and acatalasemia mutations were examined in 75 vitiligo patients and in 162 controls, in Hungary. We measured blood catalase activity and conducted analyses with PCR-SSCP, polyacrylamide gel electrophoresis and silver staining in combination with RFLP and nucleotide sequencing. Comparison of the wild (CC) genotype and the mutant (TT) genotype in the vitiligo patients revealed a non significant (P > 0.19) increase in blood catalase. Male controls with the CT genotype had significantly (P mutation (37C>T in exon 9) and the 113G>A (exon 9) mutation in Hungary are further proofs of genetic heterogeneity origin of acatalasemia mutations. In conclusion, the -262 C>T polymorphism has a reverse effect on blood catalase in vitiligo patients and in controls. In controls the mutant genotypes and alleles are more frequent in Hungary than in several other populations. The new acatalasemia mutations are further examples of heterogeneity of acatalasemia.

  4. Vitiligo Lateral Lower Lip

    Directory of Open Access Journals (Sweden)

    Sahoo Antaryami

    2002-01-01

    Full Text Available Vitiligo characteristically affecting the lateral lower lip (LLL is a common presentation in South Orissa. This type of lesion has rarely been described in literature. One hundred eighteen such cases were studied during the period from October 1999 to September, 2000. LLL vitiligo constituted 16.39% of all vitiligo patients. Both sexes were affected equally. The peak age of onset was in the 2nd decade, mean duration of illness 21.46 months. Fifty six patients had unilateral lesion (38 on the left and 18 on the right. Among the 62 patients having bilateral lesions, the onset was more frequent on the left (38 than either the right (8 or both sides together (16. All the patients were right handed. Association with local factors like infection, trauma, cheilitis, FDE etc were associated in 38.98% of cases, but systemic or autoimmune diseases were not associated. Positive family history was found in 22% of cases.

  5. Identification of Hitherto Undefined B-Cell Epitopes by Antibodies in the Sera of Vitiligo Patients Using Phage-Display Peptide Library

    Directory of Open Access Journals (Sweden)

    Zohreh Jadali

    2003-12-01

    Full Text Available A random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. Subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. This panel was tested using an ELIS A for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient's sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phage-displayed random peptide library of vitiligo sera can reveal peptide sequences that mimic vitiligo-related self-antigen.

  6. Vitiligo disease triggers: psychological stressors preceding the onset of disease.

    Science.gov (United States)

    Silverberg, Jonathan I; Silverberg, Nanette B

    2015-05-01

    Vitiligo is the loss of skin pigmentation caused by autoimmune destruction of melanocytes. Little is known about the impact of psychological stressors preceding vitiligo onset on symptoms associated with vitiligo and the extent of disease. We performed a questionnaire-based study of 1541 adults with vitiligo to evaluate the impact of psychological stressors in this patient population. Psychological stressors should be considered as potential disease triggers in vitiligo patients, and screening of vitiligo patients for psychological stressors and associated symptoms should be included in routine assessment.

  7. Retrospective Analysis on 1367 Cases of Localized Vitiligo%1367例局限型白癜风的回顾性分析

    Institute of Scientific and Technical Information of China (English)

    唐艳丽; 许爱娥; 王涛; 郑江玲

    2011-01-01

    目的 总结和分析局限型白癜风进展的临床特征.方法 采取调查问卷方式收集1 367例局限型白癜风门诊患者的临床资料,进行整理,统计分析局限型白癜风进展的临床特征.结果 由局限型发展到散发型白癜风782例(57.21%),平均4.701年;由局限型进展到肢端型白癜风199例(14.56%),平均6.721年;局限型发展为节段型191例(13.97%),平均2.034年;局限型发展为泛发型33例(2.41%),平均7.18年;有162例(11.85%)仍是局限型白癜风.结论 大多数局限型白癜风是寻常型白癜风的早期,少部分是节段型白癜风的早期.随着时间的进展以及相关可能诱因的影响,进展为散发型白癜风占多数.局限型白癜风平均经过7~8年,能进展为泛发型白癜风.因此,白癜风应尽早治疗,控制其发展.%Objective To summarize and analyse clinical characteristics of progress of localized vitiligo. Methods Questionnaires were carried out to collect the clinical data on 1367 patients with vitiligo. Statistical analysis was completed. Results 782 cases of localized vitiligo (57.21%) progressed to sporadic vitiligo, which needed an average of 4.701 years; 199 cases of localized vitiligo (14.56%) progressed to the acral vitiligo, which needed an average of 6.721 years; 191 cases of localized vitiligo (13.97%) progressed to segment vitiligo, which needed an average of 2.03 years; and 33 cases of localized vitiligo(2.41%) progressed to generalized vitiligo, which needed an average of 7.18 years. Only 162 cases of localized vitiligo (11.85% ) remained unchanged. Conclusion Vitiligo is a progressive disease. It is concluded that most localized type vitiligo is the early stage of non-segmental type, but a small number of cases belong to the early stage of segmental type. A rapid progression of vitiligo is more likely to be observed in patients affected by other disease or factors. Most patients with localized vitiligo progress to sporadic

  8. MicroRNA-155 is Dysregulated in the Skin of Patients with Vitiligo and Inhibits Melanogenesis-associated Genes in Melanocytes and Keratinocytes.

    Science.gov (United States)

    Šahmatova, Liisi; Tankov, Stoyan; Prans, Ele; Aab, Alar; Hermann, Helen; Reemann, Paula; Pihlap, Maire; Karelson, Maire; Abram, Kristi; Kisand, Kai; Kingo, Külli; Rebane, Ana

    2016-08-23

    Little is known about the functions of microRNAs (miRNAs) in skin pigmentation disorders. The aim of this study was to investigate the expression and potential role of miRNAs in vitiligo. Of 12 studied miRNAs with proven functions in cell proliferation, differentiation, immune responses and melanogenesis, miR-99b, miR-125b, miR-155 and miR-199a-3p were found to be increased and miR-145 was found to be decreased in the skin of patients with vitiligo. Combined pathway and target analysis revealed melanogenesis-associated targets for miR-99b, miR-125b, miR-155 and miR-199a-3p. In situ hybridization analysis demonstrated increased expression of miR-155 in the epidermis of patients with vitiligo. Correspondingly, miR-155 was induced by vitiligo-associated cytokines in human primary melanocytes and keratinocytes. When overexpressed, miR-155 inhibited the expression of melanogenesis-associated genes and altered interferon-regulated genes in melanocytes and keratinocytes. In conclusion, this study demonstrates that the expression of miRNAs is dysregulated in the skin of patients with vitiligo and suggests that miR-155 contributes to the pathogenesis of vitiligo.

  9. Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.

    Directory of Open Access Journals (Sweden)

    Andries Blokzijl

    Full Text Available The diagnosis of malignant melanoma currently relies on clinical inspection of the skin surface and on the histopathological status of the excised tumor. The serum marker S100B is used for prognostic estimates at later stages of the disease, but analyses are marred by false positives and inadequate sensitivity in predicting relapsing disorder.To investigate SOX10 as a potential biomarker for melanoma and vitiligo.In this study we have applied proximity ligation assay (PLA to detect the transcription factor SOX10 as a possible serum marker for melanoma. We studied a cohort of 110 melanoma patients. We further investigated a second cohort of 85 patients with vitiligo, which is a disease that also affects melanocytes.The specificity of the SOX10 assay in serum was high, with only 1% of healthy blood donors being positive. In contrast, elevated serum SOX10 was found with high frequency among vitiligo and melanoma patients. In patients with metastases, lack of SOX10 detection was associated with treatment benefit. In two responding patients, a change from SOX10 positivity to undetectable levels was seen before the response was evident clinically.We show for the first time that SOX10 represents a promising new serum melanoma marker for detection of early stage disease, complementing the established S100B marker. Our findings imply that SOX10 can be used to monitor responses to treatment and to assess if the treatment is of benefit at stages earlier than what is possible radiologically.

  10. Serum levels of homocysteine, folate and vitamin B12 in patients with vitiligo before and after treatment with narrow band ultraviolet B phototherapy and in a group of controls.

    Science.gov (United States)

    Ataş, Hatice; Cemil, Bengü Çevirgen; Gönül, Müzeyyen; Baştürk, Eda; Çiçek, Emel

    2015-07-01

    The association between vitamin B12, folate, homocysteine and vitiligo were studied in several studies, but the results are contradictory. Narrow-band ultraviolet B (NBUVB) phototherapy is now considered as a gold standard for the treatment of diffuse vitiligo. The effects of NBUVB phototherapy on both vitamin B12, folate and homocysteine levels have not been studied in vitiligo patients yet. Serum levels of vitamin B12, folate and homocysteine were measured in vitiligo patients and control group and also both before and after NBUVB phototherapy in vitiligo patients. While levels of homocysteine in patients with vitiligo were significantly higher than controls (16.9±8.4 vs. 10. 9±3.4 μmol/L; p0.05). NBUVB phototherapy led to a 33.7±21.9% (0-75%) response in patients with vitiligo after 80 seccions. Treatment with NBUVB improved vitiligo and decreased serum levels of vitamin B12 (375±151 vs. 346±119 pg/ml, p=0.024), while serum levels of folate and homocysteine did not change significantly after treatment (p=0.914, p=0.127). Further studies are needed to clarify the influence of NBUVB phototherapy on folate, vitamin B12 and homocysteine levels in patients with vitiligo. Furthermore, studies with the analysis of skin levels of homocysteine rather than circulating levels may be useful to elucidate the effects of phototherapy on homocysteine levels.

  11. Pentachrome vitiligo.

    Science.gov (United States)

    Fargnoli, M C; Bolognia, J L

    1995-11-01

    The term "trichrome vitiligo" has been used to describe lesions of vitiligo that have an intermediate level of color between the achromic center and the peripheral unaffected skin. We describe a 12-year-old black girl with a generalized pigmentary disorder that first appeared when she was 3 years of age. Five shades of colors--white, tan, medium brown (unaffected skin), dark brown, and black--were present. Biopsy specimens of the depigmented areas showed a complete absence of melanocytes, whereas the most hyperpigmented areas were characterized by an increase in epidermal pigment and number of melanocytes. The lack of any preceding inflammation in the affected areas and the subsequent widespread spontaneous depigmentation that occurred within 1 year pointed to a diagnosis of "pentachrome vitiligo."

  12. American Vitiligo Research Foundation

    Science.gov (United States)

    ... life can acquire vitiligo Welcome to The American Vitiligo Foundation 2017 AVRF Calendars Order your calendar with ... animal testing. Please Visit Our Donations Page American Vitiligo Research Foundation "We Walk By Faith, Not By ...

  13. Genetic Susceptibility to Vitiligo: GWAS Approaches for Identifying Vitiligo Susceptibility Genes and Loci

    Directory of Open Access Journals (Sweden)

    Chang eShen

    2016-02-01

    Full Text Available Vitiligo is an autoimmune disease with a strong genetic component, characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Genetic factors are known to play key roles in vitiligo through discoveries in association and family studies. Previously, vitiligo susceptibility genes were mainly revealed through linkage analysis and candidate gene studies. Our understanding of the genetic basis of vitiligo has been rapidly advancing through genome-wide association studies (GWASs. More than 40 robust susceptible loci have been identified and confirmed to be associated with vitiligo by using GWASs. Most of these associated genes participate in important pathways involved in the pathogenesis of vitiligo, such as immunoregulatory function, melanocyte regulation and so on. A number of susceptible loci with unknown functions in the pathogenesis of vitiligo have also been identified, indicating that additional molecular mechanisms may contribute to the risk of developing vitiligo. In this review, we summarize the key loci that are of genome-wide significance, which have been shown to influence vitiligo risk. These genetic loci may help build the foundation for genetic diagnosis and personalize treatment for patients with vitiligo in the future. However, substantial additional studies, including gene-targeted and functional studies, are required to confirm the causality of the genetic variants and their biological relevance in vitiligo development.

  14. Genetic Susceptibility to Vitiligo: GWAS Approaches for Identifying Vitiligo Susceptibility Genes and Loci.

    Science.gov (United States)

    Shen, Changbing; Gao, Jing; Sheng, Yujun; Dou, Jinfa; Zhou, Fusheng; Zheng, Xiaodong; Ko, Randy; Tang, Xianfa; Zhu, Caihong; Yin, Xianyong; Sun, Liangdan; Cui, Yong; Zhang, Xuejun

    2016-01-01

    Vitiligo is an autoimmune disease with a strong genetic component, characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Genetic factors are known to play key roles in vitiligo through discoveries in association studies and family studies. Previously, vitiligo susceptibility genes were mainly revealed through linkage analysis and candidate gene studies. Recently, our understanding of the genetic basis of vitiligo has been rapidly advancing through genome-wide association study (GWAS). More than 40 robust susceptible loci have been identified and confirmed to be associated with vitiligo by using GWAS. Most of these associated genes participate in important pathways involved in the pathogenesis of vitiligo. Many susceptible loci with unknown functions in the pathogenesis of vitiligo have also been identified, indicating that additional molecular mechanisms may contribute to the risk of developing vitiligo. In this review, we summarize the key loci that are of genome-wide significance, which have been shown to influence vitiligo risk. These genetic loci may help build the foundation for genetic diagnosis and personalize treatment for patients with vitiligo in the future. However, substantial additional studies, including gene-targeted and functional studies, are required to confirm the causality of the genetic variants and their biological relevance in the development of vitiligo.

  15. Study of Oxidative Stress in Vitiligo

    OpenAIRE

    2010-01-01

    Vitiligo is an idiopathic, acquired, circumscribed, hypomelanotic skin disorder, characterized by milky white patches of different sizes and shapes. It is due to the destruction of melanocytes resulting in the absence of pigment production of the skin and mucosal surfaces. Oxidative stress has been implicated in pathophysiology of vitiligo. To study the activity of blood Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in vitiligo patients. A case–control study was conducted in whi...

  16. Clinical analysis of 387 patients with segmental vitiligo%387例节段型白癜风患者的临床分析

    Institute of Scientific and Technical Information of China (English)

    李阳; 祝逸平; 许爱娥

    2013-01-01

    Objective To characterize the progression of segmental vitiligo.Methods Clinical data were collected using questionnaires from 387 patients with segmental vitiligo at the Department of Dermatology,Third People's Hospital of Hangzhou,between October 2011 and October 2012.The progression of segmental vitiligo was analyzed.Results Among the 387 patients,329 initially sufferred from focal vitiligo that evolved into segmental vitiligo later,58 began with segmental vitiligo.Vitiligo progressed most rapidly in the initial three months,and tended to be stable after three years in 220 (66.9%) of the 329 patients experiencing the evolution from focal to segmental vitiligo.By contrast,vitiligo developed most rapidly in the first month,and tended to be quiescent after one year in 40 (69.0%) of the 58 patients presenting with segmental vitiligo only.The condition still remained active in 27.6% (107/387) of these patients after several years of progression.Totally,333 (86.0%) patients showed the involvement of single ganglion at the onset of vitiligo,including 173 (52.0%) patients with the involvement of trigeminal ganglion.Autologous melanocyte transplantation was conducted for 62 patients whose condition had been quiescent for more than one year,and 56 (90.3%) patients achieved more than 80% repigmentation.Conclusions Segmental vitiligo,which tends to remain quiescent after three-year progression,seems to have a more rapid onset than focal vitiligo.Autologous melanocyte transplantation appears to be an effective treatment for segmental vitiligo in stable stage.%目的 探讨节段型白癜风的病情发展特征.方法 问卷调查方法收集2011年10月至2012年10月于我科门诊就诊的387例节段型白癜风患者的资料,进行分析.结果 387例中329例(85.0%)发病由局限型发展为节段型,58例首发为节段型.在局限型发展为节段型患者中,220/329例(66.9%)在发病前3个月进展最快,进展3年趋于稳定.在58/387例(15

  17. Camouflage for vitiligo.

    Science.gov (United States)

    Tanioka, Miki; Miyachi, Yoshiki

    2009-01-01

    Cosmetic camouflage is indispensable for patients with vitiligo and can result in an improvement of their quality of life. Recent cosmetic advances enabled camouflage to obtain a suitable color match and keep it waterproof. However, camouflage needs some techniques. Therefore, patient education through a camouflage lesson is required to enjoy camouflage. Here the authors introduced a few tips for suitable camouflage, which were devised through camouflage lessons.

  18. Original article title: "Comparison of therapeutic efficacy of topical corticosteroid and oral zinc sulfate-topical corticosteroid combination in the treatment of vitiligo patients: a clinical trial"

    Directory of Open Access Journals (Sweden)

    Omidian Mohammad

    2011-03-01

    Full Text Available Abstract Background Vitiligo is the most prevalent pigmentary disorder which occurs worldwide, with an incidence rate between 0.1-4 percent. It is anticipated that the discovery of biological pathways of vitiligo pathogenesis will provide novel therapeutic and prophylactic targets for future approaches to the treatment and prevention of vitiligo. The purposes of this study were evaluating the efficacy of supplemental zinc on the treatment of vitiligo. Methods This randomized clinical trial was conducted for a period of one year. Thirty five patients among 86 participants were eligible to entrance to the study. The patients in two equal randomized groups took topical corticosteroid and combination of oral zinc sulfate-topical corticosteroid. Results The mean of responses in the corticosteroid group and the zinc sulfate-corticosteroid combination group were 21.43% and 24.7%, respectively. Conclusion Although, the response to corticosteroid plus zinc sulfate was more than corticosteroid, there was no statistically significant difference between them. It appeared that more robust long-term randomized controlled trials on more patients, maybe with higher doses of zinc sulfate, are needed to fully establish the efficacy of oral zinc in management of vitiligo. Trial Registration chiCTRTRC10000930

  19. Prevalência de psoríase em estudo de 261 pacientes com vitiligo Prevalence of psoriasis in a study of 261 patients with vitiligo

    OpenAIRE

    2005-01-01

    FUNDAMENTOS: O estudo da associação de psoríase e vitiligo é necessário em razão das prováveis origens imunológicas dessas enfermidades e da proximidade de loci encontrados no cromossomo 1p31. OBJETIVO: O propósito principal foi determinar a prevalência de psoríase em pacientes com vitiligo em amostra de 740 pacientes submetidos à fototerapia em Curitiba, PR e descrever suas características clínicas. MÉTODOS: Dos 740, foram estudados retrospectivamente 261 pacientes com diagnóstico de vitilig...

  20. Positivity of autologous serum skin test in patients with alopecia areata and vitiligo and in healthy individuals

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    Münevver Güven

    2015-09-01

    Full Text Available Background and Design: Autologous serum skin test (ASST, the best in-vivo test displaying in vitro basophil histamin releasing activity, is used in the diagnosis of chronic autoimmune urticaria. Besides, it is cheap and is easy to perform. It has been found that in ASST-positive chronic urticaria patients, autoimmune thyroid disease especially and other autoimmune diseases were more common and the level of autoimmune markers were higher compared to others. Autoimmunity is accused in the pathogenesis of alopecia areata and vitiligo. In this study, we assessed ASST results in healthy controls and those with autoimmune diseases, and aimed to explore the effects of thyroid autoantibodies and other factors in ASST positivity. Materials and Methods: ASST was administered to 51 patients with alopecia areata, 53 patients with vitiligo and 51 healthy controls, and thyroid function tests and thyroid autoantibodies (anti-Tg, anti-TPO were assessed. Results: ASST was positive in 64.7% of patients with in alopecia areata, 64.2% of those with vitiligo and in 45.1% of controls. There was no statistically significant difference between the groups in terms of ASST positivity. We observed that ASST positivity had no relationship with age, anti-Tg, anti-TPO and the presence of one or both autoantibody positivity. It was seen that the frequency of ASST positivity was higher in females than in men in all groups, but it was statistically significant in alopecia areata group only. Among the all study groups, the frequency of ASST positivity was statistically significantly higher in females than in men. Conclusion: The high rates of ASST positivity in individuals with alopecia areata and vitiligo as well as in healthy control, indicate that ASST positivity does not solely exist in chronic urticaria patients. With logical regression analysis, it was shown that, having alopecia areata and being female significantly increase the risk of having ASST positivity. Therefore

  1. Randomized controlled study to evaluate the effectiveness of dexamethasone oral minipulse therapy versus oral minocycline in patients with active vitiligo vulgaris

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    Akhilendra Singh

    2014-01-01

    Full Text Available Background: Oral minocycline has been recently shown to halt disease progression in active vitiligo. Aims: The present study was planned to compare the efficacy and tolerability of oral minocycline with oral mini pulse (OMP corticosteroids in active vitiligo. Methods: A total of 50 patients with actively spreading vitiligo were randomized to receive either minocycline 100 mg/day (Group I - 25 patients or OMP 2.5 mg dexamethasone on 2 consecutive days in a week (Group II - 25 patients for 6 months. These were followed-up at every 2 weeks interval. Mean vitiligo disease activity score (VIDA and mean Vitiligo Area Scoring Index (VASI were assessed in all patients in addition to the photographic comparison before and after treatment. Results: Both groups showed a significant decrease in VIDA from 4.0 to 1.64 ± 0.86 (P < 0.001 in Group I and from 4.0 to 1.68 ± 0.69 (P < 0.001 in Group II. However, the difference between the mean VIDA scores in the two groups was not statistically significant (P = 0.60 at the end of treatment period. The mean VASI declined from 1.71 ± 1.45 to 1.52 ± 1.43 Group I (P = 0.06 and from 1.39 ± 1.31 to 1.17 ± 1.34 in Group II (P = 0.05. The difference between VASI in Group I and II was not significant at the end of 24 weeks of treatment (P = 0.11. Conclusion: Both dexamethasone OMP and oral minocycline are effective drugs for managing the arrest of disease activity in vitiligo.

  2. Action of topical mometasone on the pigmented halos of micrografting in patients with vitiligo Ação da mometasona tópica nos halos pigmentares de microenxertia em vitiligo

    Directory of Open Access Journals (Sweden)

    Karine Dantas Diógenes Saldanha

    2012-10-01

    Full Text Available BACKGROUND: Vitiligo is a prevalent skin pigmentation disorder worldwide. The treatments available still offer limited results to some patients. For patients with clinically stable vitiligo, melanocyte transplantation is an appropriate treatment option, and the technique of autologous punch grafting shows good repigmentation. OBJECTIVE: To evaluate the effect of topical mometasone on the halos of repigmentation after autologous punch grafting in patients with clinically stable vitiligo. METHODS: Between 2009 and 2010, 11 patients with clinically stable vitiligo (7 generalized, 2 focal and 2 segmental underwent autologous punch grafting in the achromic patches. According to the clinical type of vitiligo, patients were instructed to use the corticosteroid ointment during 6 months, only on a few grafted lesions. In the first month, the mometasone ointment was used twice a day and after that just once. They were reassessed 1, 3 and 6 months after the procedure. Grafted halos were photographed and recorded using the software fotofinder. After 6 months, all the treated and untreated areas of the repigmentation halos were measured and analyzed comparatively. RESULTS: The median area of the repigmentation halos after 6 months of treatment with mometasone was larger (25,96 mm² than the one of the untreated halos (13,86 mm² , showing a statistically significant difference (p = 0,026. CONCLUSION: In this study, the use of mometasone ointment increased the area of the repigmentation halos after punch grafting. However, this should be further investigated in larger samples in order to validate this positive action in the treatment of stable vitiligo.FUNDAMENTOS: Vitiligo é um transtorno de pigmentação freqüente na população mundial. Seu tratamento ainda oferece resultados limitados em alguns pacientes. Nos casos de vitiligo estável clinicamente, o transplante de melanócitos tornase uma opção terapêutica, sendo a técnica de enxertos aut

  3. Depigmentation therapies in vitiligo

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    Divya Gupta

    2012-01-01

    Full Text Available Depigmentation therapy in vitiligo is an option in those with extensive vitiligo who have failed to respond to medical therapy and have obvious cosmetic disfigurement due to intervening patchy pigmented areas. Various aspects of this therapy such as the cost, treatment time, course, permanency of depigmentation, side effects, and the possibility of repigmentation should first be discussed with the patient. At present, there is no ideal depigmenting therapy available, but many agents in the market have been in use for many years. Monobenzyl ether of hydroquinone (MBEH is the mainstay and Food and Drug Administration (FDA approved in USA but takes many months to depigment and is associated with local side effects and risk of repigmentation. Other agents which are also used are 4-methoxy phenol and 88% phenol. Physical therapies for depigmentation include Q-switched ruby and alexandrite lasers and cryotherapy. Second-line agents which can be explored for depigmentation include imatinib mesylate, imiquimod, and diphencyprone. Many possible experimental agents are being explored like various phenol derivatives, melanoma vaccines, interferon gamma, busulfan, etc. A major lacuna still exists in this area and a lot more research is desirable to give satisfactory cosmesis to these patients with extensive vitiligo.

  4. Reduction of Blister Formation Time in Suction Blister Epidermal Grafting in Vitiligo Patients Using a Household Hair Dryer.

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    Arora, Shweta; Kar, Bikash Ranjan

    2016-01-01

    Suction blister epidermal grafting (SBEG) is a simple and effective way of surgical repigmentation in vitiligo. The major problem faced is the time taken for the formation of blisters. Temperature at the suction site is one of the factors affecting the blister formation time. To reduce the blister formation time in SBEG by increasing the surface temperature to 44°C. This is a left-right comparison study. Total seven patients with lip vitiligo involving both the angles of lips were enrolled. Suction syringes were applied on both the thighs of all the patients. On the right thigh, blisters were raised as per the procedure standardised by Gupta et al. On the left thigh, similar procedure was used, but a hair dryer was used additionally to increase the surface temperature of the skin to 44°C. The time taken for the formation of well-formed, dome-shaped, unilocular blister was noted. The mean time taken for the formation of blister on the right thigh was 121.1 ± 6.2 min and on the left thigh was 69.6 ± 5.4 min. All the seven patients were started on PUVASOL after SBEG. There was complete repigmentation of the grafted sites in all the patients after 2 months. Hair dryer is easily available, affordable and simple to use and the time saved during the procedure is quite significant.

  5. Association study between nonsense polymorphism (rs2039381, Gln71Stop) of interferon-ε and susceptibility to vitiligo in Korean population.

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    Cho, Hee-Ryung; Kim, Su Kang; Lim, Hee-Kyeong; Jeong Park, Hae; Chung, Joo-Ho; Lee, Mu-Hyoung

    2013-01-01

    Interferons (IFNs) are related to autoimmune responses. IFN-epsilon (IFNE) is included in IFN family, and may modulate immunological functions. Inflammation modulating functions of IFNE may be related with the pathophysiology of vitiligo. To investigate the association of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-ε (IFNE) and susceptibility to vitiligo, we conducted a case-control association study in 265 non-segmental vitiligo (NSV) patients and 320 healthy controls. The nonsense single nucleotide polymorphism (SNP) (rs2039381, Gln71Stop) of IFNE was genotyped by direct sequencing. Multiple logistic regression models (log-additive, dominant, and recessive models) were applied to determine odds ratios (OR), 95% confidence interval (CI), and p values. The rs2039381 (Gln71Stop) of IFNE did not show significant differences between NSV patient group and control group. However, we found that in childhood onset NSV groups, the IFNE nonsense polymorphism (rs2039381, Gln71Stop) showed a significant association. There was significantly different distribution of nonsense polymorphism of rs2039381 (Gln71Stop) of IFNE between NSV patients (childhood vitiligo in NSV patients.

  6. A descriptive study to characterize segmental vitiligo

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    Binod K Khaitan

    2012-01-01

    Full Text Available Background: Segmental vitiligo is a small but unique subset of vitiligo requiring due importance due to its lack of response to medical treatment but excellent response to surgical treatment. Characterization of the pattern of segmental vitiligo will also help to understand the pathogenesis of the disease. Aim: To characterize clinically the features of segmental vitiligo, a cross-sectional clinical study at dermatology outpatient department at AIIMS was carried out. Methods: Consecutive 188 patients were evaluated to characterize the clinical features of segmental vitiligo by detailed history, clinical examination, and photography. Frequency of each clinical feature was calculated. Results: Certain features such as early onset, initial progression of disease followed by stability, blaschkoid pattern, irregular margins, leucotrichia within and beyond the vitiligo lesion, and islands of pigmented macules within the vitiligo lesion were found to be characteristic of the disease. Conclusions: A combination of various features such as early onset of disease, blaschkoid pattern, irregular margins, leucotrichia, and islands of pigmented macules within the vitiligo lesion are helpful in diagnosis of the disease.

  7. Changes of epidermal thickness in vitiligo.

    Science.gov (United States)

    Jung, Soo-Eun; Kang, Hee Young; Lee, Eun-So; Kim, You Chan

    2015-04-01

    The stratum corneum and epidermal pigmentation have protective roles against ultraviolet radiation. Because vitiligo skin lacks melanocytes and has no potential to produce pigment, some studies suggested that the epidermis in vitiligo skin is thicker than in normal skin. However, only a few studies investigated epidermal thickness changes in vitiligo, and some of these had relatively small sample sizes. Thus, this study aimed to compare epidermal thickness between vitiligo skin and adjacent normal-appearing skin in a large cohort. Photos of hematoxylin and eosin–stained slides of vitiligo skin and adjacent normal-appearing skin were taken under a microscope. The thicknesses of the stratum corneum, viable epidermis, and full epidermis were then measured by a computerized image analyzer. A total of 206 patients (412 sections) were included. There were significant differences between vitiligo skin and adjacent normal-appearing skin in the thickness of the stratum corneum (P = 0.009), viable epidermis (P = 0.001), and total epidermis (P = 0.001). An analysis comparing skin biopsied from a sun-exposed area versus a sun-protected area showed that the stratum corneum, viable epidermis, and total epidermis were significantly thicker in vitiligo skin than in normal-appearing skin in sun-exposed areas (P vitiligo skin than in normal-appearing skin, especially on sun-exposed skin, and that this may represent a photoprotective role compensating for absent pigmentation.

  8. New insights into immune mechanisms of vitiligo.

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    Boniface, Katia; Taïeb, Alain; Seneschal, Julien

    2016-02-01

    Vitiligo is the most common depigmenting disorder, affecting 0.5% of the population. This stigmatizing disease has a major social impact with high unmet needs, and no real curative intervention has been reported so far. Vitiligo is characterized by the development of white macules resulting from a loss of epidermal melanocytes, which can result from cell destruction through melanocyte-specific cytotoxic immune response and melanocyte detachment through a defective adhesion system. Multiple mechanisms have been suggested to be involved in melanocyte disappearance: genetic predisposition, environmental triggers, metabolic abnormalities, altered inflammatory and immune responses. The autoimmune and inflammatory theory is the leading hypothesis. Indeed, vitiligo is often associated with autoimmune diseases; genome-wide association studies and functional pathway analyses have shown that most vitiligo susceptibility loci encode components of the immune system; and immune cells are found in the perilesional margin of actively depigmenting skin of vitiligo patients. However, studies support melanocytes intrinsic abnormalities in vitiligo associated with increased melanocytes stress leading to the release of dangers signals important for the activation of the immune system. This review aimed to overview the link between cellular stress, melanocyte function, and the abnormal inflammatory immune response in vitiligo. The involvement of innate and adaptive immune cells in the pathomechanisms leading to melanocyte loss observed in vitiligo will be discussed.

  9. Clinical study of nail changes in vitiligo.

    Science.gov (United States)

    Anbar, Tag; Hay, Rania Abdel; Abdel-Rahman, Amal T; Moftah, Noha H; Al-Khayyat, Mohamed A

    2013-03-01

    Both vitiligo and alopecia areata (AA) are associated together, associated with other autoimmune diseases, and autoimmunity is one of the important theories in their etiology. Nail changes are a known association with AA, thus we hypothesized that nail changes can be found in vitiligo patients. On revising the literature, only two types of nail changes were described in association with vitiligo. Our aim was to study the frequency and types of nail changes among vitiligo patients in comparison with normal healthy volunteers. This multi-centric study was carried on 91 patients with vitiligo, as well as 91 normal healthy control subjects who were age- and sex-matched. Nails were examined for changes in nail plates as regards striations, texture, curvature, dystrophy, and pigmentation. The presence or absence of the thumb lunula was also reported. Nail changes were observed in 62 patients (68.1%) and 46 (50.5%) control subjects with a statistically significant difference (P = 0.016). Longitudinal ridging and absent lunula were significantly higher in patients than in the controls (P = 0.001 and 0.037, respectively). Other reported nail abnormalities in the current study included punctate leukonychia, pitting, flag sign, and Terry's nails. Awareness of this association will widen the clinician's perspective to carefully examine the nail changes in vitiligo patients and conversely examine patients with nail changes for vitiligo.

  10. Determination of plasma levels of interleukin-17 in patients with vitiligo%白癜风患者血浆IL-17水平的检测

    Institute of Scientific and Technical Information of China (English)

    苗青; 于伟; 程亚南; 贾名妍; 常爱武

    2014-01-01

    目的 探讨白癜风与IL-17的相关性.方法 酶联免疫吸附测定法对32例白癜风患者和30例健康对照组外周血血浆IL-17含量进行检测,分析IL-17水平与白癜风患者不同分期、面积、病程的关系.结果 进展期和稳定期白癜风患者血浆IL-17含量与健康对照组相比明显升高(均P<0.05),且进展期明显高于稳定期(P< 0.05);IL-17水平与皮损面积呈正相关(r=0.456,P<0.05),与病程无相关(r=0.239,P> 0.05).结论 IL-17在白癜风的发生发展过程中起到一定作用.%Objective To investigate the relationship between interleukin-17 (IL-17) and vitiligo.Methods Totally,32 vitiligo patients and 30 healthy human controls were enrolled in this study.Blood samples were collected from all the subjects,and enzyme-linked immunosorbent assay (ELISA) was performed to determine the plasma levels of IL-17.The relationship of plasma IL-17 levels with disease stage,clinical course and lesion area was assessed.Results The plasma levels of IL-17 were significantly higher in the patients with progressive and stable vitiligo than in the healthy controls (both P < 0.05),and higher in the patients with progressive vitiligo than in those with stable vitiligo (P < 0.05).Moreover,the plasma levels of IL-17 were positively correlated with the area of vitiligo lesions (r =0.456,P < 0.05),but unrelated to the clinical course of vitiligo (r =0.239,P > 0.05).Conclusion IL-17 may play a certain role in the occurrence and development of vitiligo.

  11. Plasma levels of interleukin-17, interleukin-23, and transforming growth factor-β in Sudanese patients with vitiligo: A case-control study

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    Ali Malik Osman

    2015-01-01

    Full Text Available Background: Vitiligo is the most common pigmentary skin disorder. It is a multifactorial polygenic disease with epidermal melanocyte destruction. The cytokines profile found in vitiliginous patients was not fully elucidated. Aims: We sought to assess the autoimmune nature of vitiligo by comparing plasma levels of interleukin (IL-17, IL-23, and transforming growth factor beta (TGF-b in adult Sudanese vitiligo patients with matched control individuals. Subjects and Methods: Case-control study was conducted in Khartoum Dermatologic Teaching Hospital, in the period between July and December 2013. The cases were 42 adult Sudanese vitiligo patients matched with 43 control individuals. The cytokines were measured in the plasma by the quantitative "sandwich" ELISA. Results: Patients showed a significant lower median (25-75 th inter-quartile of TGF-β than control (0.042 [0.041-0.044] vs. 0.047 [0.042-0.049]; P ͳ 0.001. Both IL-17 and IL-23 showed no significant difference between cases and controls. IL-17 showed a significant inverse relationship when correlated with TGF-β (r = −0.24; P = 0.026 while showing direct relationship when correlated with age (r = 0.28; P = 0.009. Conclusion: The positive findings detected in this study coincide with the important immunoregulatory role of the TGF-β, and support the autoimmune nature of the disease.

  12. Vitiligo: A part of a systemic autoimmune process

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    Gopal KVT

    2007-01-01

    Full Text Available Background : Recent clinical and animal experimental studies postulate that the pathogenetic mechanisms of vitiligo could be of systemic origin as vitiligo is associated with ocular and auditory abnormalities as well as other autoimmune disorders.Hence, we studied genetic factors, systemic associations, ocular and auditory abnormalities of vitiligo. Methods: The study group included 150 new cases of various types of vitiligo. One hundred age- and sex-matched nonvitiligo cases were included as controls in the study. A complete family history was taken for all patients. Examination was carried out taking note of the type of vitiligo and approximate percentage of body surface involved. All relevant laboratory investigations, a thorough audiological examination including pure tone audiometry and a complete ophthalmologic examination were carried out in all patients and controls. Statistical analysis was done using the Chi square test. Results: Fifty-four vitiligo patients (36% had a family history of vitiligo. Anemia was present in 30 (20% vitiligo patients but only in five (5% controls, a difference that was statistically significant (c2 = 15.8, P < 0.001. Diabetes mellitus was present in 24 (16% vitiligo patients and only 2 (2% of controls (Chi square, c2 = 12.4, P < 0.001. Hypothyroidism and alopecia areata were present in 18 (12% and 11 (7.4% vitiligo patients respectively and none of the controls. Hypoacusis was seen in 30 (20% vitiligo patients and two (2% controls (c2 = 8.19, P < 0.005. Twenty-four vitiligo patients (16% and five controls (5% had specific ocular abnormalities like uveitis, iris and retinal pigmentary abnormalities (c2 = 7.39, P < 0.001. Conclusion: This study demonstrates statistically significant clinical evidence confirming that vitiligo is a part of systemic autoimmune process.

  13. Oxidative stress drives CD8(+) T-cell skin trafficking in patients with vitiligo through CXCL16 upregulation by activating the unfolded protein response in keratinocytes.

    Science.gov (United States)

    Li, Shuli; Zhu, Guannan; Yang, Yuqi; Jian, Zhe; Guo, Sen; Dai, Wei; Shi, Qiong; Ge, Rui; Ma, Jingjing; Liu, Ling; Li, Kai; Luan, Qi; Wang, Gang; Gao, Tianwen; Li, Chunying

    2017-07-01

    In patients with vitiligo, an increased reactive oxygen species (ROS) level has been proved to be a key player during disease initiation and progression in melanocytes. Nevertheless, little is known about the effects of ROS on other cells involved in the aberrant microenvironment, such as keratinocytes and the following immune events. CXCL16 is constitutively expressed in keratinocytes and was recently found to mediate homing of CD8(+) T cells in human skin. We sought to explicate the effect of oxidative stress on human keratinocytes and its capacity to drive CD8(+) T-cell trafficking through CXCL16 regulation. We first detected putative T-cell skin-homing chemokines and ROS in serum and lesions of patients with vitiligo. The production of candidate chemokines was detected by using quantitative real-time PCR and ELISA in keratinocytes exposed to H2O2. Furthermore, the involved mediators were analyzed by using quantitative real-time PCR, Western blotting, ELISA, and immunofluorescence. Next, we tested the chemotactic migration of CD8(+) T cells from patients with vitiligo mediated by the CXCL16-CXCR6 pair using the transwell assay. CXCL16 expression increased and showed a positive correlation with oxidative stress levels in serum and lesions of patients with vitiligo. The H2O2-induced CXCL16 expression was due to the activation of 2 unfolded protein response pathways: kinase RNA (PKR)-like ER kinase-eukaryotic initiation factor 2α and inositol-requiring enzyme 1α-X-box binding protein 1. CXCL16 produced by stressed keratinocytes induced migration of CXCR6(+)CD8(+) T cells derived from patients with vitiligo. CXCR6(+)CD8(+) T-cell skin infiltration is accompanied by melanocyte loss in lesions of patients with vitiligo. Our study demonstrated that CXCL16-CXCR6 mediates CD8(+) T-cell skin trafficking under oxidative stress in patients with vitiligo. The CXCL16 expression in human keratinocytes induced by ROS is, at least in part, caused by unfolded protein response

  14. What Is Vitiligo?

    Science.gov (United States)

    ... the skin are destroyed. These cells are called melanocytes (ma-LAN-o-sites). Vitiligo can also affect ... In vitiligo, the immune system may destroy the melanocytes in the skin. It is also possible that ...

  15. Vitiligo, drug induced (image)

    Science.gov (United States)

    ... this person's face have resulted from drug-induced vitiligo. Loss of melanin, the primary skin pigment, occasionally ... is the case with this individual. The typical vitiligo lesion is flat and depigmented, but maintains the ...

  16. Identification of Novel HLA-A*0201-Restricted CTL Epitopes in Chinese Vitiligo Patients

    Science.gov (United States)

    Cui, Tingting; Yi, Xiuli; Guo, Sen; Zhou, Fubo; Liu, Ling; Li, Chunying; Li, Kai; Gao, Tianwen

    2016-01-01

    Generalized vitiligo is an autoimmune disease characterized by melanocyte loss, which results in patchy depigmentation of skin and hair. Recent studies suggested the key role of CD8+T lymphocytes for mediating immune response in vitiligo through melanocyte differentiation antigens, including tyrosinase, gp100 and MelanA/Mart-1. However, the specific epitopes of these auto-antigens are still unknown. In our study, we predicted the possible HLA-A*0201-restricted nonapeptides overlaying the full-length amino acid sequences of these three known antigens and investigated the lymphocytes reactivity to these nonapeptides by Elispot assay. In addition, we evaluated the abilities of these nonapeptides to activate CD8+T cells. We screened out 5 possible epitopes originated from tyrosinase and gp100, numbered P28, P41, P112, P118 and P119. Among these 5 epitopes, notably, P28 and P119 played the dominant role in activating CTLs, with a significant increase in proliferation rate and Interferon-γ (IFN-γ) production of CD8+T cells. Nevertheless, antigen-specific T cell reactivity was not detected in MelanA/Mart-1 peptides. Our studies identified two novel epitopes originated from proteins of gp100 and tyrosinase, which may have implications for the development of immunotherapies for vitiligo. PMID:27821860

  17. Diphencyprone Induced Vitiligo: A Case Report

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    Mohammad Ali Nilforoushzadeh

    2012-01-01

    Full Text Available Diphencyprone (DCP is a contact sensitizer which is used to treat dermatological disorders with an immunological origin, such as extensive alopecia areata (AA. Vitiligo is a rare but known side effect of DPCP therapy which is formed in the treatment site or remote areas. In this paper a 37-year-old man developed alopecia totalis with loss of eyebrows and eyelashes who presented some vitiligo patches on his scalp and arm distant from the location of DPCP application and a 42-year-old woman with 25 years history of hair loss and 3 months DPCP application who revealed some vitiligo patches on the scalp with distant to the site of application at the 6th week are reported. Considering the absence of personal and family history of Vitiligo in our two cases, the hypothesis of latent Vitiligo is not proved. The positive patch test in left arm of one of the patients also suggests the direct role of DPCP as the cause of Vitiligo occurrence. As the development of vitiligo by DCP is unpredictable and the depigmentation may persist indefinitely, it is important to inform all patients about this potential adverse effect before starting the treatment.

  18. 白癜风光疗疗效与临床的关系%Relationship between the efficacy of phototherapy and clinical factors in patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    邱实; 许爱娥

    2013-01-01

    白癜风是一种色素脱失性疾病,以皮肤或黏膜色素减退为特征,多为后天发病,可见于任何年龄,以青壮年多见,部分患者有明显的季节性,一般春末夏初病情加重,冬季缓解.任何部位均可发病,但好发于暴露部位.该病治疗困难,影响美观,给患者带来精神心理压力.目前运用较广泛的有308 nm准分子激光、窄谱中波紫外线和氦氖激光等,临床应用发现,光疗的疗效与白癜风分型、部位、病程、治疗次数、皮肤类型和红斑反应等相关.%Vitiligo is a pigmentary disorder characterized by cutaneous or mucous hypopigmentation.It is usually acquired,and may occur at any age,but is more common in young adults.In some patients with vitiligo,the condition gets worse at late spring and early summer,but better in winter.It may affect any body sites with a predilection for exposed sites.Vitiligo can bring psychical and mental stress to patients by affecting their appearance,however,it still remains difficult to treat.At present,commonly used phototherapy for vitiligo includes 308-nm excimer laser,narrow band-ultraviolet B,helium neon laser,etc.Clinical practice has suggested that the efficacy of phototherapy is associated with the type,site,clinical course of vitiligo,as well as treatment sessions,skin types and erythema reaction of patients.

  19. Surgical treatment in acrofacial vitiligo

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    Shah Bharat

    1994-01-01

    Full Text Available A total 18 patients of stationary acrofacial vitiligo, relatively resistant to medical treatment, of various age were selected for blister grafting. Duration of their disease varied from 3 to 10 years. The autografts were taken up and cosmetically acceptable result were seen in approximately 90% of the cases during the follow up period varying from 6 months to 2 years.

  20. Vitiligo following type II lepra reaction.

    Science.gov (United States)

    Pavithran, K

    1989-01-01

    A middle-aged male with lepromatous leprosy developed bouts of skin lesions of depigmented macules and patches of vitiligo, just following attacks of type II lepra reaction each time. In view of the present concept of autoimmunity playing a role in the pathogenesis of vitiligo as well as lepra reaction, their association in our patient appears to be more than fortuious. The depigmented macules persisted even after regression of skin lesions of leprosy following chemotherapy. The vitiligo macules responded partially to topical and systemic psoralen therapy.

  1. Vitiligo: Pathogenesis, clinical variants and treatment approaches.

    Science.gov (United States)

    Iannella, Giannicola; Greco, Antonio; Didona, Dario; Didona, Biagio; Granata, Guido; Manno, Alessandra; Pasquariello, Benedetta; Magliulo, Giuseppe

    2016-04-01

    Vitiligo is a common chronic acquired disease of pigmentation whose etiology is unknown, which usually occurs with asymptomatic whitish patch or macule. Although several hypotheses have been proposed in the literature, the leading theory is still the auto-immune etiology linked to specific genetic mutations. Vitiligo can also be associated with several autoimmune diseases, including autoimmune thyroid diseases, alopecia areata, and halo nevi. Sensorineural hearing loss was reported in several vitiligo patients due to a reduction in the number of melanocytes contained in the membranous labyrinth of the inner ear. Because of its complexity, several therapeutic options are available to treat this systemic disease.

  2. Vitiligo and autoantibodies of celiac disease

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    Zabihollah Shahmoradi

    2013-01-01

    Conclusion: There may be a relationship between celiac disease and vitiligo. This may indicate a common basic autoimmune mechanism that is an explanation for few case reports that gluten free diets were effective in the treatment of vitiligo patients. Both T test and exact fisher test showed no effect of age, sex and job on seropositivity of these patients (P = 0.56 and P = 0.74, respectively

  3. Development and pilot testing of a vitiligo screening tool.

    Science.gov (United States)

    Sheth, Vaneeta M; Gunasekera, Nicole S; Silwal, Sujeeta; Qureshi, Abrar A

    2015-01-01

    Studies aimed at understanding the pathology, genetics, and therapeutic response of vitiligo rely on asking a single question about 'physician-diagnosed' vitiligo on surveys to identify subjects for research. However, this type of self-reporting is not sufficient. Our objective was to determine if the patient-administered Vitiligo Screening Tool (VISTO) is a sensitive and specific instrument for the detection of vitiligo in an adult population. The VISTO consists of eight closed-ended questions to assess whether the survey participant has ever been diagnosed with vitiligo by a healthcare worker and uses characteristic pictures and descriptions to inquire about the subtype and extent of any skin lesions. 159 patients at the Brigham and Women's Hospital dermatology clinic with or without a diagnosis of vitiligo were recruited. A board-certified dermatologist confirmed or excluded the diagnosis of vitiligo in each subject. 147 completed questionnaires were analyzed, 47 cases and 100 controls. The pictorial question showed 97.9% sensitivity and 98% specificity for diagnosis of vitiligo. Answering "yes" to being diagnosed with vitiligo by a dermatologist and choosing one photographic representation of vitiligo showed 95.2% sensitivity and 100% specificity for diagnosis of vitiligo. We conclude that VISTO is a highly sensitive and specific, low-burden, self-administered tool for identifying vitiligo among adult English speakers. We believe this tool will provide a simple, cost-effective way to confirm vitiligo prior to enrollment in clinical trials as well as for gathering large-scale epidemiologic data in remote populations. Future work to refine the VISTO is needed prior to use in genotype-phenotype correlation studies.

  4. Clinical analysis of 693 patients with Vitiligo%白癜风693例临床特征分析

    Institute of Scientific and Technical Information of China (English)

    葛善珍; 卢涛

    2015-01-01

    Objective To summarize the clinical characteristics and causative factors of vitiligo, and provide basis for the prevention and treatment of vitiligo. Methods 693 cases of vitiligo cases data of 2013 August to 2014 August received treatment in the Department of Dermatology, Department of our hospital were collected, the main content includes general information and clinical data, using the SPSS19.0 and Excel tables for statistical analysis. Results 353 cases were male ( 50.94%),female 340 cases (49.06%). The average age of (25.29+1.04) years of age. The peak age of onset ranged from 12 to 19 years old. Most patients with occupation high school students, 178 cases (25.69%);543 cases of patients with vitiligo vulgaris (78.35%);502 cases (72.44%) in the advanced stage;423 cases (61.04%) in the spring and summer disease. Lesions in the exposure site (the head and face, neck and hand foot part) high incidence (52.79%). The average duration was (5.15+0.27) years, less than 5 years of 484 people (69.84%);The inducing factors, 44.30% (307/693)was affected by the psychological factors of the patients,75 (10.82%) patients with other skin diseases, 23 patients had a history of trauma, 23 cases had a positive family history. Conclusion vitiligo is a chronic skin disease, good hair at young people, men and women the ratio of similar, more to an unusual type of onset,spring and summer high season, skin lesions with exposure and the friction parts of the more common, the sun,psychological factors and injury is common inducement of vitiligo occurred.%目的:总结分析白癜风的临床特征及诱发因素,为白癜风的防治提供依据。方法:对2013年8月-2014年8月就诊于笔者医院皮肤科门诊的693例白癜风病例资料收集整理,主要内容包括一般资料和临床资料,运用SPSS19.0和Excel表格进行统计分析。结果:男性353例(50.94%),女性340例(49.06%);平均发病年龄(25.29±1.04)岁,发病年龄高峰为12~19岁

  5. Significant immediate and long-term improvement in quality of life and disease coping in patients with vitiligo after group climatotherapy at the Dead Sea.

    Science.gov (United States)

    Krüger, Christian; Smythe, Jim W; Spencer, Jennifer D; Hasse, Sybille; Panske, Angela; Chiuchiarelli, Giorgia; Schallreuter, Karin U

    2011-03-01

    Quality of life in patients with vitiligo is impaired. This study explored the immediate effect of 20 days of climatotherapy at the Dead Sea on quality of life, coping with the disease, general well-being and individual stress levels in a group of 71 patients with vitiligo and 42 matched controls. The long-term effect was assessed after 12 months in 33/71 patients and 12/42 controls. Study instruments were Dermatology Life Quality Index, Beck Depression Inventory and the Adjustment to Chronic Skin Disorders Questionnaire. Stress measurements were based on cortisol and β-endorphin concentrations in saliva samples. Quality of life was significantly improved at day 20 at the Dead Sea compared with day 1, and this was still significant after 12 months. Moreover, social anxiety/avoidance, anxious-depressive mood and helplessness as measured by the Adjustment to Chronic Skin Disorders Questionnaire were significantly reduced. There was no difference in levels of cortisol and β-endorphin between patients and controls, indicating that stress per se is not a significant contributor in vitiligo. In conclusion, therapy in patient groups offers an effective tool for long-lasting improvement in quality of life and patients' well-being.

  6. Adult Onset Vitiligo: Multivariate Analysis Suggests the Need for a Thyroid Screening.

    Science.gov (United States)

    Lazzeri, L; Colucci, R; Cammi, A; Dragoni, F; Moretti, S

    2016-01-01

    Background. There are limited epidemiological studies evaluating the effect of age at onset on disease features in vitiligo. Objectives. To identify factors associated with adult onset vitiligo in comparison with childhood onset vitiligo. Patients and Methods. We retrospectively collected medical records of 191 patients. Such records included clinical examination, personal and familial medical history, laboratory evaluations, concomitant vitiligo treatment and drug assumption. Results. 123 patients with a disease onset after the age of 40 (adult onset vitiligo) were compared with 68 patients who developed vitiligo before the age of 12 (childhood onset vitiligo). Multivariate analysis revealed that personal history of thyroid diseases (P = 0.04; OR 0.4), stress at onset (P = 0.002; OR = 0.34), personal history of autoimmune thyroid disease (ATD) (P = 0.003; OR = 0.23), and thyroid nodules (P = 0.001; OR 0.90) were independently associated with adult onset vitiligo, whereas family history of dermatological diseases (P = 0.003; OR = 2.87) and Koebner phenomenon (P < 0.001; OR = 4.73) with childhood onset vitiligo. Moreover, in the adult onset group, concomitant thyroid disease preceded vitiligo in a statistically significant number of patients (P = 0.014). Conclusions. Childhood onset and adult onset vitiligo have different clinical features. In particular, ATD and thyroid nodules were significantly associated with adult onset vitiligo, suggesting that a thyroid screening should be recommended in this group of patients.

  7. Childhood vitiligo: Treatment paradigms

    Directory of Open Access Journals (Sweden)

    Amrinder Jit Kanwar

    2012-01-01

    Full Text Available Childhood vitiligo differs from the adults by showing a higher incidence in females, segmental vitiligo being more common and less frequent association with other systemic autoimmune and endocrine disorders.Childhood vitiligo is often associated with a marked psychosocial and long lasting effect on the self-esteem of the affected children and their parents, hence an adequate treatment is very essential. Treatment of vitiligo is indeed a tough challenge for the dermatologists′ more so in the background of childhood vitiligo. Although multiple therapeutic modalities are available in the therapeutic armamentarium, not all can be used in children. This brief report updates regarding various therapies available in the treatment of childhood vitiligo.

  8. Genetic analysis of 1005 patients with vitiligo%1005例白癜风患者遗传因素的调查

    Institute of Scientific and Technical Information of China (English)

    刘琬; 常建民

    2010-01-01

    目的 探讨白癜风患者发病过程中遗传因素所起的作用.方法 采用问卷调查方法收集1997年9月至2009年3月于我科门诊就诊的1005例白癜风确诊患者的临床资料,应用SPSS 13.0软件对所得数据进行统计学分析.结果 本调查的1005例白癜风先证者中,有家族史者206例,占20.5%.家族史阳性者的平均发病年龄(24.45±15.87岁)比家族史阴性者(28.12±16.88岁)小(P<0.05).家族史阳性者皮损双侧分布的比例比家族史阴性者高(71.3%比60.8%)(P<0.05).家族史阳性者以B型血居多(19.9%),家族史阴性者以O型血居多(16.8%)(P>0.05).结论 ①白癜风的发病符合多基因遗传规律,具有家族聚集性,且血缘关系愈近,其发病率愈高.②白癜风的发病与性别无关.③家族史阳性者的平均发病年龄较家族史阴性者小,父系或母系遗传对发病年龄无影响.④家族史阳性者皮损双侧分布的比例较家族史阴性者高,而对其他临床表现无影响.%Objective To evaluate the role of genetic factors in the pathogenesis of vitiligo. Methods A questionnaire survey was performed to collect clinical data on 1005 cases of vitiligo in Beijing Hospital from September 1997 to March 2009. Data analysis was carried out by SPSS 13.0 software. Results There were 206 (20.5%) patients with positive family history among the 1005 cases of vitiligo. The mean age at onset was younger and bilateral vitiligo lesions were more frequently observed in patients with positive family history than those without (24.45 ± 15.87 years vs 28.12 ± 16.88 years, 71.3% vs 60.8%, both P < 0.05). Blood type B predominated among patients with positive family history (19.9%), while blood type O among those without ( 16.8% ), and no statistical difference was noted in the distribution of blood type between the two groups of patients (P > 0.05). Conclusions ①The incidence of vitiligo accords with the rules of polygenic inheritance and shows a familial

  9. Vitiligo at Injection Site of PEG-IFN-α 2a in Two Patients with Chronic Hepatitis C: Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    V. Arya

    2010-08-01

    Full Text Available A 72-year-old female and a 57-year-old male with chronic hepatitis C were treated with a combination therapy of pegylated interferon (PEG-IFN-α 2a (180 µg s.c. once a week and ribavirin (1,000 mg orally daily. This resulted in the destruction of melanocytes at the injection site in both patients. In the male patient, the depigmentation progressed to the surrounding skin area. The dermatologist concurred with vitiligo as the diagnosis in both patients. Injection and surrounding site vitiligo associated with PEG-IFN-α 2b treatment for hepatitis C was noticed in previous case studies. For the first time, the case reports below highlight the same immunological adverse event secondary to PEG IFN-α 2a/ribavirin combination therapy and explain, in part, the complex interaction between host immune response and viral genotype. In addition, we systematically review drug-induced vitiligo and autoimmune diseases associated with the depigmentation disorder.

  10. Impact of Vitiligo on Quality of Life.

    Science.gov (United States)

    Morales-Sánchez, M A; Vargas-Salinas, M; Peralta-Pedrero, M L; Olguín-García, M G; Jurado-Santa Cruz, F

    2017-09-01

    Vitiligo is a chronic autoimmune skin disease caused by the destruction of melanocytes. Although quality of life (QOL) in vitiligo has been studied in different countries, it has not yet been investigated in Mexico. The aim of this study was to assess the QOL of Mexican patients with vitiligo. We conducted a cross-sectional study at the research unit of Centro Dermatológico Dr. Ladislao de la Pascua in Mexico City. We included adults with vitiligo and excluded those with other pigmentation disorders or a neurological or psychiatric disorder. Patients on psychoactive medications were also excluded. All the patients were administered the Dermatology Life Quality Index (DLQI), a vitiligo-specific quality of life instrument (the VitiQoL), and the Beck Depression and Anxiety Inventories. We studied 150 patients with vitiligo (103 women [68.7%] and 47 men [31.3%]). The median (interquartile range) age was 38 (20) years. The mean (SD) scores on the DLQI and VitiQoL were 5.2 (5.4) and 32.1 (22.7) out of total possible scores of 30 and 90, respectively. The correlation between questionnaire scores was 0.675 (P<.001). Patients with genital involvement scored significantly worse on the VitiQoL than those without lesions in this area (43.95 [28.4]) vs. 28.98 [20.08], P<.001). The prevalence of depression and anxiety was 34% and 60%, respectively. Vitiligo has a minimal impact on the QOL of our patients. QOL was worse in patients with genital lesions. Copyright © 2017 AEDV. Publicado por Elsevier España, S.L.U. All rights reserved.

  11. The epidemiology of iron-deficiency anemia and risk factors in the patients with vitiligo%白癜风患者缺铁性贫血流行病学调查及危险因素分析

    Institute of Scientific and Technical Information of China (English)

    程校衔; 何渊民; 张倩; 高天文; 李春英

    2016-01-01

    目的::确定白癜风患者缺铁性贫血( IDA)患病率及危险因素。方法:收集400例白癜风患者与360名普通人临床资料及血液标本,比较两组IDA患病率。结果:相比较于普通人群组,白癜风患者中IDA患病率明显升高( P<0.05)。其中,儿童及成年女性白癜风患者中IDA高于普通人群组(P<0.05)。进展期白癜风中IDA患病率明显高于稳定期白癜风(P<0.05)。结论:白癜风患者易并发IDA,机体抗氧化能力下降更为明显。%Objective:To determine the prevalence of iron-deficiency anemia ( IDA) and the risk factors in the patients with vitiligo. Methods: The clinical data and blood samples of 400 vitiligo patients and 360 matched normal people were collected. The prevalence of IDA between the two groups was compared and ana ̄lyzed. Results:Compared with the normal people, the prevalence of IDA increased significantly in vitiligo pa ̄tients ( P<0.05) . The prevalence of IDA in children and adult female patients was higher than those in normal people (P<0.05).The prevalence of IDA in the patients with progressive vitiligo was significantly higher than that in stable vitiligo ( P<0.05) . Conclusion:Vitiligo patients were prone to suffering from IDA, and the an ̄tioxidant capacity decreased more significantly in vitiligo patients with IDA.

  12. 白癜风患者外周血CD4+CD25+调节性T细胞的检测%Detection of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    白明辉; 王竞; 涂彩霞; 张蕴颖; 刘敏; 李国艳; 钟良瑞

    2009-01-01

    Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P>0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P >0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P > 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.%目的 检测不同病期白癜风患者外周血CD4+CD25+调节性T细胞水平,探讨其与白癜风发病的关系.方法 白癜风患者34例,进展期19例,稳定期15例.通过流式细胞仪对不同病期白癜风患者外周血CD4+、CD4+CD25+T细胞水平进行检测,并与20例正常人比较.结果 进展期患者外周血中CD4+CD25+调节性T细胞占外周血淋巴细胞的表达率低于正常对照组(P<0.05);稳定期患者与正

  13. Vitiligo occurring after thyroidectomy at sites of leprosy lesions

    Directory of Open Access Journals (Sweden)

    George Anuja

    1992-01-01

    Full Text Available A 51-year-old female patient developed vitiligo at the previous sites of treated leprosy immediately after thyroidectomy. A neurological factor in association with thyroid dysfunction is considered as the possible aetiology of vitiligo in this case.

  14. Standard guidelines of care for vitiligo surgery

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    Parsad Davinder

    2008-03-01

    Full Text Available Vitiligo surgery is an effective method of treatment for selected, resistant vitiligo patches in patients with vitiligo. Physician′s qualifications: The physician performing vitiligo surgery should have completed postgraduate training in dermatology which included training in vitiligo surgery. If the center for postgraduation does not provide education and training in cutaneous surgery, the training may be obtained at the surgical table (hands-on under the supervision of an appropriately trained and experienced dermatosurgeon at a center that routinely performs the procedure. Training may also be obtained in dedicated workshops. In addition to the surgical techniques, training should include local anesthesia and emergency resuscitation and care. Facility: Vitiligo surgery can be performed safely in an outpatient day care dermatosurgical facility. The day care theater should be equipped with facilities for monitoring and handling emergencies. A plan for handling emergencies should be in place, with which all nursing staff should be familiar. Vitiligo grafting for extensive areas may need general anesthesia and full operation theater facility in a hospital setting and the presence of an anesthetist is recommended in such cases. Indications for vitiligo surgery : Surgery is indicated for stable vitiligo that does not respond to medical treatment. While there is no consensus on definitive parameters for stability, the Task Force suggests the absence of progression of disease for the past one year as a definition of stability. Test grafting may be performed in doubtful cases to detect stability. Preoperative counseling and Informed consent: A detailed consent form elaborating the procedure and possible complications should be signed by the patient. The patient should be informed of the nature of the disease and that the determination of stability is only a vague guide. The consent form should specifically state the limitations of the procedure

  15. Failure of alefacept in the treatment of vitiligo.

    Science.gov (United States)

    Bin Dayel, Salman; AlGhamdi, Khalid

    2013-02-01

    Vitiligo is a common acquired pigmentary disorder with a profound psychosocial impact. The exact pathogenesis of vitiligo is not fully understood; however, vitiligo appears to be an autoimmune disease involving T-cell-mediated melanocyte destruction. Recently, complete clearance of coexisting vitiligo without recurrence over 2 years was reported in 2 psoriasis patients treated with alefacept. To evaluate the safety and efficacy of alefacept in the treatment of vitiligo. After providing informed written consent, 4 adult patients with widespread vitiligo (covering a body surface area ≥5%) were treated with weekly intramuscular injections of 15 mg alefacept for 12 weeks. All patients were monitored clinically, by laboratory investigation, and by digital image analysis. All patients were followed up with for 24 weeks. All patients tolerated alefacept well, without any adverse events. None of the patients showed any repigmentation. However, 1 patient developed new depigmented patches during treatment with alefacept. A pilot study with a small number of patients. Alefacept as a monotherapy for vitiligo treatment did not result in any patient improvement, and further evaluation in larger studies may be required.

  16. Redução dos linfócitos T-CD8+ citotóxicos observada com a terapia Puva em paciente com vitiligo Reduction of cytotoxic T-CD8+ lymphocytes observed during PUVA therapy in a patient with vitiligo

    Directory of Open Access Journals (Sweden)

    Daniela Pereira Antelo

    2008-12-01

    Full Text Available Na patogênese do vitiligo tem-se enfatizado o papel das células T citotóxicas. Identificadas pelo antígeno linfocitário cutâneo (CLA, essas células já foram descritas no sangue de pacientes com outras dermatoses e podem ser depletadas pela fototerapia concomitantemente à melhora clínica. Descreve-se caso de vitiligo generalizado com melhora clínica expressiva após Puva, no qual houve redução de 25% dos linfócitos T CD8+-CLA+ circulantes.The role of cytotoxic T cells (CD8+ has been emphasized in the pathogenesis of vitiligo. This cell population, identified by a skin homing molecule (cutaneous lymphocyte associated antigen Â- CLA, has been described in patients with other dermatoses and can be reduced with phototherapy. The authors present the case of a patient with generalized vitiligo and clinical recovery after PUVA parallel to a 25% reduction in CD8+-CLA+ T cells in the peripheral blood.

  17. Hepatitis C Virus Antibodies and Vitiligo Disease

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    Z Jadali

    2005-06-01

    Full Text Available Vitiligo is a common skin disorder, characterized by depigmented patches due to selective destruction of melanocytes. The etiology of this disease is unknown. A number of hypotheses including viral theory have been proposed to explain the etiology. To determine the prevalence of antibody to hepatitis C virus infection in vitiligo patients, the present study was performed. Third generation ELISA test was used for detection of antibodies to HCV in human sera. All normal controls were anti-HCV negative whereas only one patient was positive for anti-HCV and there was no significant difference in the prevalence of anti-HCV between patients and controls. These results indicate that hepatitis C virus has not a direct causal role in the pathogenesis of vitiligo, however, this does not rul out a "hit and run" virus induced disease.

  18. Acknowledging popular misconceptions about vitiligo in western Saudi Arabia

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    Mohammad I. Fatani

    2016-01-01

    Full Text Available Background: Vitiligo is the most common depigmentary disorder of the skin and hair. Our aim is to evaluate knowledge, attitudes, and misconceptions about vitiligo among adults attending shopping centres. Methods: A cross-sectional study design was implemented. It included a representative sample of adults in Jeddah and Makkah who presented in shopping centres every weekend during March, 2014. Results: The study included 423 subjects aged between 18 and 65 years with a mean of 29.9 ± 9.7 years. Females represent 70% of them. Only 6.9% of the participants heard well about vitiligo, with social media (32.2% the most commonly reported source of information. Overall, vitiligo knowledge was sufficient in 41.8% of the participants. Females had a significantly higher knowledge score compared with males, and older subjects (31–50 and >50 years had a higher knowledge score compared with younger subjects (18–30 years, which proved statistically significant. Attitudes towards vitiligo were positive in 57.4% of participants. Conclusion: Knowledge of vitiligo in adults is suboptimal, yet attitude towards the disease is generally acceptable. Educating the public about vitiligo could lead to increased self-confidence, better social integration, and psychological well-being for vitiligo patients.

  19. Immunohistochemical detection of P53 and Mdm2 in vitiligo

    Science.gov (United States)

    Bakry, Ola A.; Hammam, Mostafa A.; Wahed, Moshira M. Abdel

    2012-01-01

    Background: Vitiligo is a common depigmented skin disorder that is caused by selective destruction of melanocytes. It is generally accepted that the main function of melanin resides in the protection of skin cells against the deleterious effect of ultraviolet rays (UVRs). Association of vitiligo and skin cancer has been a subject of controversy. Occurrence of skin cancer in long-lasting vitiligo is rare despite multiple evidences of DNA damage in vitiliginous skin. Aim: To detect the expression of P53 and Mdm2 proteins in both depigmented and normally pigmented skin of vitiligo patients and to compare it to control subjects suffering from nonmelanoma skin cancer (NMSC). Materials and Methods: Thirty-four patients with vitiligo and 30 age and sex-matched patients with nodulo-ulcerative basal cell carcinoma (BCC) as a control group were selected. Both patients and control subjects had outdoor occupations. Skin biopsies were taken from each case and control subjects. Histopathological examination of Hematoxylin and eosin-stained sections was done. Expression of P53 and Mdm2 proteins were examined immunohistochemically. Results: Both P53 and Mdm2 were strongly expressed in depigmented as well as normally pigmented skin of vitiligo patients. This expression involved the epidermis, skin adnexa and blood vessels with significant differences between cases and controls. Conclusions: The overexpression of P53 and Mdm2 proteins in both normally pigmented and depigmented skin of patients with vitiligo could contribute to the decreased occurrence of actinic damage and NMSC in these patients. PMID:23189248

  20. Becker nevus with vitiligo and lichen planus: Cocktail of dermatoses

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    Sanjeev Gupta

    2010-07-01

    Full Text Available Context: Becker nevus has been reported to be associated with lichen planus (LP in isolated case reports in past. The association of LP and vitiligo has been noted in few cases and has been attributed to a common autoimmune etiology. The coexistence of lichen planus, vitiligo and Becker nevus has not been reported so far. Case Report: A thirty five years old male presented with lesions of Becker nevus along with vitiligo and Lichen planus coexisting at one place on right side of the chest. Vitiligo and Becker nevus could not be treated. Lichen planus was confirmed histopathologically. We were able to treat lichen planus with topical potent steroids, tacrolimus and systemic antihistamines. The vitiligo lesion in our case was resistant to treatment. Conclusion: This case is being reported for the rare occurrence of three different well defined skin conditions in our patient and reviews the possible known etiological factors for their coexistence.

  1. Vitiligo, con énfasis en su variante inflamatoria Vitiligo, with emphasis in its inflammatory variant

    Directory of Open Access Journals (Sweden)

    C I Vera

    2009-03-01

    Full Text Available El vitiligo inflamatorio es un trastorno melanocitopénico adquirido, de baja frecuencia con características clínicas e histológicas propias. Ocurre en ambos sexos, a cualquier edad. Su fisiopatogenia parece involucrar mecanismos autoinmunes. En su evolución es frecuente la desaparición del componente inflamatorio que resulta en una mácula hipopigmentada clásica; a ello debe anticiparse también nuestra elección terapéutica. Se describe un paciente con vitiligo inflamatorio de resolución espontánea, junto a una revisión crítica de la bibliografía. Se discute si representa una variante de vitiligo vulgar o una entidad independiente.Inflammatory vitiligo is a low frequency melanocytopenic acquired entity with clinical and histological features of it 's own. It occurs in both sexes at any age. Fisiopathogenesis may involve autoimmune mechanisms. It's evolution most commonly ends up in a classic hypopigmented maculae, therefore our therapeutic choice has to be oriented to this outcome. A patient with inflammatory vitiligo is described along a critical review of the literature. It is discussed if it represents a variant of common vitiligo or an independent entity.

  2. Phototherapy for the treatment of vitiligo in Asian children.

    Science.gov (United States)

    Koh, Mark Jean-Aan; Mok, Zhun-Rui; Chong, Wei-Sheng

    2015-01-01

    Vitiligo is a common acquired progressive depigmenting condition that can have devastating psychological effects in dark-skinned patients. We performed a retrospective review of patients younger than 16 years of age with a clinical diagnosis of vitiligo treated using phototherapy at the National Skin Center, Singapore, over a 5-year period. Seventy-one Asian patients ages 5 to 15 years when they underwent phototherapy were identified. There was a higher proportion of Indian patients in our cohort than in the population. The duration of disease ranged from 2 months to 12 years. More than half of the patients had generalized vitiligo and more than one-third had segmental vitiligo. Patients with generalized vitiligo had a better response than those with segmental vitiligo. Reported response rates were highest for narrowband ultraviolet B (UVB) phototherapy, followed by targeted phototherapy combining ultraviolet A1 (UVA1) and UVB; 308-nm excimer lamp phototherapy and paint psoralen-UVA photochemotherapy had marginally lower reported response rates. The duration of treatment ranged from 3 to 40 months and the total number of treatments ranged from 20 to 209 sessions. Reported side effects were mild and included itching, scaling, erythema, pain, sunburn, blistering, and phototoxicity. We consider phototherapy to be a safe and efficacious modality for the treatment of vitiligo in Asian children.

  3. Quality of life impairment in children and adolescents with vitiligo.

    Science.gov (United States)

    Silverberg, Jonathan I; Silverberg, Nanette B

    2014-01-01

    Vitiligo significantly affects quality of life (QOL) in adults, but little is known about the effect on QOL of pediatric vitiligo and whether the extent, distribution, and duration of vitiligo are associated with QOL. We performed an online parental questionnaire-based study (N = 350) regarding children ages 0 to 17 years with vitiligo, including validated questions about body surface area (BSA), distribution, and age of onset of vitiligo, associated symptoms, and QOL using the Children's Dermatology Life Quality Index (CDLQI). Vitiligo negatively affected numerous aspects of and total CDLQI score (median 3.0, interquartile range 5.0). Their vitiligo lesions did not bother only 4.1% of teenagers ages 15 to 17 years, versus 45.6% of children ages 0 to 6 years and 50.0% of those ages 7 to 14 years (p sites of vitiligo lesions for children and parents were the face (25.6% and 37.4%, respectively) and legs (26.2% and 26.2%, respectively). Eighty-two patients (30.1%) reported itching and painful skin within the past week. Using multivariate ordinal logistic regression models, it was found that an affected BSA of more than 25% was associated with self-consciousness, difficulty with friendships and schoolwork, and teasing and bullying. Lesions on the face and arms were associated with teasing and bullying. The extent of vitiligo is associated with QOL impairment in children and adolescents, especially self-consciousness, but also bullying and teasing. Different distributions of vitiligo lesions are associated with different aspects of QOL impairment. Teenagers ages 15 to 17 years seem to experience the most self-consciousness of all pediatric age groups.

  4. CLINICAL EPIDEMIOLOGICAL PROFILE OF VITILIGO

    African Journals Online (AJOL)

    2012-08-08

    Aug 8, 2012 ... Conclusion: Vitiligo affects all ages with more females affected. Vitiligo vulgaris is the commonest ... The age of onset is usually in childhood or early adulthood with peak incidence in the second and third decade of life (3).

  5. A figurate papulosquamous variant of inflammatory vitiligo.

    Science.gov (United States)

    Lee, D; Lazova, R; Bolognia, J L

    2000-01-01

    The classic description of inflammatory vitiligo is an erythematous rim at the periphery of a patch of hypopigmented or depigmented skin. The histological correlate is a superficial perivascular infiltrate of mononuclear cells. However, we observed a 61-year-old patient with inflammatory vitiligo who had thin solid pink scaly plaques as well as serpiginous lesions with fine scale. Histologically, a lichenoid infiltrate was seen as was a thickened stratum corneum with parakeratosis. In a review of the literature, scattered case reports of similar findings were identified, either in the Japanese and French literature or from over 25 years ago. The clinical and histological spectrum of inflammatory vitiligo should be expanded to include solid and annular papulosquamous plaques as well as lichenoid infiltrates with exocytosis.

  6. Increased oxidative DNA damage in mononuclear leukocytes in vitiligo

    Energy Technology Data Exchange (ETDEWEB)

    Giovannelli, Lisa [Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy)]. E-mail: lisag@pharm.unifi.it; Bellandi, Serena [Department of Dermatological Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy); Pitozzi, Vanessa [Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy); Fabbri, Paolo [Department of Dermatological Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy); Dolara, Piero [Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy); Moretti, Silvia [Department of Dermatological Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence (Italy)

    2004-11-22

    Vitiligo is an acquired pigmentary disorder of the skin of unknown aetiology. The autocytotoxic hypothesis suggests that melanocyte impairment could be related to increased oxidative stress. Evidences have been reported that in vitiligo oxidative stress might also be present systemically. We used the comet assay (single cell alkaline gel electrophoresis) to evaluate DNA strand breaks and DNA base oxidation, measured as formamidopyrimidine DNA glycosylase (FPG)-sensitive sites, in peripheral blood cells from patients with active vitiligo and healthy controls. The basal level of oxidative DNA damage in mononuclear leukocytes was increased in vitiligo compared to normal subjects, whereas DNA strand breaks (SBs) were not changed. This alteration was not accompanied by a different capability to respond to in vitro oxidative challenge. No differences in the basal levels of DNA damage in polymorphonuclear leukocytes were found between patients and healthy subjects. Thus, this study supports the hypothesis that in vitiligo a systemic oxidative stress exists, and demonstrates for the first time the presence of oxidative alterations at the nuclear level. The increase in oxidative DNA damage shown in the mononuclear component of peripheral blood leukocytes from vitiligo patients was not particularly severe. However, these findings support an adjuvant role of antioxidant treatment in vitiligo.

  7. Evaluation and treatment of vitiligo

    NARCIS (Netherlands)

    Vrijman, C.

    2015-01-01

    This thesis considers several aspects of vitiligo; it investigates (i) provoking factors of vitiligo, (ii) the prevalence of thyroid disease, (iii) the efficacy and safety of autologous punch- and cell suspension grafting and (iv) measurement properties of outcome measures for vitiligo. Chapter 2.1

  8. Anti-thyroid peroxidase antibody and vitiligo: a controlled study

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    Akhyani Maryam

    2006-03-01

    Full Text Available Abstract Background Vitiligo is an acquired depigmenting disorder due to destruction of melanocytes. Although many theories have been suggested for its pathogenesis, the role of autoimmunity is the most popular one. The association of vitiligo with autoimmune thyroid diseases and the increased prevalence of autoantibodies including thyroid autoantibodies in vitiligo favor this role. Our objective was to compare the frequency of thyroid peroxidase antibody (anti-TPO in vitiligo patients with healthy subjects in Iran. Methods Ninety-four cases of vitiligo (46 female and 48 male and 96 control subjects (49 female and 47 male were enrolled in this controlled study. Patients with known thyroid disease, history of thyroid surgery and those receiving thyroid medications were not included. The two groups were matched regarding gender and age. The demographic data, symptoms related to thyroid diseases and results of skin and thyroid examinations were recorded in a questionnaire for each subject. Thyroid function tests including free T3, free T4 and TSH-IRMA were performed. Anti-TPO levels were assessed as well. The collected data were analyzed by SPSS version-11 in vitiligo patients and subgroups according to gender, age, extent, and duration of the disease compared with the control group. Results Anti-TPO was detected in 17 (18.1% of patients affected by vitiligo, while this figure was 7 (7.3% in the control group; the difference was significant with p-value The difference of the frequency of anti-TPO was not significant regarding the duration and extent of vitiligo. In addition, there was no significant difference in the levels of free T3, free T4, and TSH in vitiligo patients compared with the control group. Conclusion According to our study, anti-TPO was shown to be significantly more common in vitiligo patients especially in young women, compared with control group. As this antibody is a relatively sensitive and specific marker of autoimmune thyroid

  9. Etiopathogenesis of vitiligo : Are we dealing with an environmental disorder ?

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    Behl P

    1999-01-01

    Full Text Available The increasing incidence of vitiligo in this part of the world (4.25%, prompted us to study 5000 fresh vitiligo patients. Several triggering factors were elicitable. Malnutrition and intake of junk food were very common in childhood vitiligo. Intercurrent infections as well as intake of antibotics were also significant, immediately preceding the development of depigmented patches in younger age group. Genetic predisposition was uncommon. Autoimmune disorders were infrequent and usually accompanied late onset vitiligo. For management, vitiligo was classified into VI (active, V2 (quiescent and V3 (improving stages. The therapy administered varied in each stage. As a common denominator, all out efforts were made to eliminate possible trigger factors in individual cases throughout the period of treatment, and building the general health to prevent recurrence in future. The study strongly points out that vitiligo is a multifactorial disorder. It can be effectively managed after each patient is individually assessed. Elimination of possible triggering factors may form the mainstay of vitiligo therapy along with controlled pharmacological intervention. The improvement of general resistance of body may bring spontaneous repigmentation.

  10. Immunolocalization of tenascin-C in vitiligo.

    Science.gov (United States)

    Abdou, Asmaa Gaber; Maraee, Alaa Hassan; Shoeib, Mohamed Abd El-Monaem; Elbana, Rania

    2012-10-01

    The disappearance of melanocytes because of defective adhesion is one of the accepted theories to explain vitiligo. Tenascin-C is a large, extracellular matrix glycoprotein that is thought to inhibit adhesion of melanocytes to fibronectin. The current study aimed to evaluate the pattern of tenascin-C expression in vitiligenous skin compared with normal pigmented skin by means of immunohistochemistry. The study was carried out on skin biopsies from lesional and perilesional skin of 30 patients with vitiligo and on normal skin of 10 healthy volunteers. Several histopathologic changes were observed in vitiliginous skin such as keratinocyte vacuolization, a thickened basement membrane, and dermal inflammatory changes. Tenascin-C was expressed in keratinocytes of the basal epidermal layer of normal skin biopsies at a mild intensity but it did not stain the dermis, whereas vitiligenous skin showed tenascin-C expression in most cases (93.3% ), in the papillary dermis, epidermis, and in both. Diffuse epidermal expression of tenascin-C correlated with more loss of pigment and continuous staining of tenascin-C in the papillary dermis correlated with progressive forms of vitiligo. Intense tenascin-C expression was associated with a more progressive course of the disease assessed by the vitiligo disease activity score. From this study, tenascin-C is highly expressed in the dermis, epidermis, and both of vitiligo as a secondary event for the disease. Keratinocyte is a source of tenascin-C in vitiligo, and diffuse epidermal expression of tenascin-C may induce more loss of melanocytes and melanin pigment. Dermal expression of tenascin-C in the vitiligenous lesion may be linked to the disease more than epidermal expression, because this pattern is only seen in a vitiligenous lesion and it is completely absent in normal and perilesional skin.

  11. Target-like pigmentation after minipunch grafting in stable vitiligo

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    Nelee Bisen

    2014-01-01

    Full Text Available Surgical treatment for vitiligo has been ever evolving. Each surgical modality has its own benefits and limitations. Miniature punch grafting is the most extensively performed surgery, which gives good results in stable vitiligo. Herein we report an unusual type of repigmentation observed after minipunch grafting in a patient of stable vitiligo, which resembled target-like lesions with a "perigraft halo" surrounding individual grafts. Such pigment spread occurred despite the use of 0.5 mm larger graft from the donor site.

  12. Accidental PUVA burns, vitiligo and atopic diathesis resulting in prurigo nodularis: a logical but undocumented rarity*

    Science.gov (United States)

    Verma, Shyam Bhanushankar; Wollina, Uwe

    2012-01-01

    Vitiligo is a dreaded disease in India due to its social and cultural consequences. PUVA and PUVAsol are the main treatment modalities for vitiligo vulgaris. To the best of our knowledge, this is the first case of accidental PUVA burns eventuating in prurigo nodularis lesions to be reported in a female patient who was undergoing home PUVA therapy. The itch is so prominent and disabling that the focus of the patient has shifted from treating her vitiligo to ameliorating the pruritus. PMID:23197209

  13. The role of vitamin D in melanogenesis with an emphasis on vitiligo

    OpenAIRE

    Khalid AlGhamdi; Ashok Kumar; Noura Moussa

    2013-01-01

    Vitiligo is a common pigmentary disorder caused by the destruction of functional melanocytes. Vitamin D is an essential hormone synthesized in the skin and is responsible for skin pigmentation. Low levels of vitamin D have been observed in vitiligo patients and in patients with other autoimmune diseases. Therefore, the relationship between vitamin D and vitiligo needs to be investigated more thoroughly. We reviewed the literature to date regarding the role of vitamin D in skin pigmentation. O...

  14. Serum miRNA expression profiles change in autoimmune vitiligo in mice.

    Science.gov (United States)

    Shi, Yu-Ling; Weiland, Matthew; Lim, Henry W; Mi, Qing-Sheng; Zhou, Li

    2014-02-01

    It is widely believed that non-segmental vitiligo results from the autoimmune destruction of melanocytes. MicroRNAs (miRNAs), a class of small non-coding RNAs that negatively regulate gene expression, are involved in the immune cell development and function and regulate the development of autoimmune diseases. Recent studies demonstrate that functional miRNAs can be detected in the serum and serve as biomarkers of various diseases. In the present study, we used a mouse autoimmune vitiligo model, in which melanocyte autoreactive CD4+ T cells were adoptively transferred into Rag1(-/-) host mice. Serum miRNA expression was profiled in vitiligo developed mice and control mice using TaqMan RT-PCR arrays. We have found that the expressions of 20 serum miRNAs were changed in vitiligo mice compared to control mice. Three increased miRNAs, miR-146a, miR-191, and miR-342-3p, were further confirmed by a single TaqMan RT-PCR. Our findings suggest that miRNAs may be involved in vitiligo development and serum miRNAs could serve as serum biomarkers for vitiligo in mice.

  15. Detection of Serum Interleukin-10 and Tumor Necrosis Factor-α in Patients with Vitiligo%白癜风患者血清IL-1O与TNF-α的检测

    Institute of Scientific and Technical Information of China (English)

    邓茂; 曾凡才; 廖勇梅; 钟桂书

    2012-01-01

    目的 探讨检测白癜风患者血清中自介素-10(IL-10)与肿瘤坏死因子-α(TNF-α)的临床指导意义.方法 随机选择2011年7月-11月本科门诊诊治的58例寻常期白癜风患者为研究组,以同期本院行健康体检者65例作为对照组.采用双抗体夹心ELISA法检测两组血清中IL-10和TNF-d的含量.比较分析两组以及研究组中不同分期患者的血清IL-10和TNF-α含量.结果 研究组患者IL-10含量低于对照组,TNF-α含量高于对照组,差异均具有统计学意义(P均<0.05),对照组和稳定期患者的IL-10含量高于进展期患者,而TNF-α含量低于进展期患者,差异均有统计学意义(P均<0.05).结论 白癜风患者血清中IL-10的含量呈下降趋势,当病情达到进展期时,呈现最低值;同时TNF-o含量呈上升趋势,当病情达到进展期时,呈现最高值.检测白癜风患者血清中IL-10与TNF-α为研究白癜风的发病机制奠定了基础.%Objective To probe the clinical guiding significance of serum interleukin-10 and tumor necrosis factor-a in patients with vitiligo. Methods Fifty-eight patients with vitiligo during unusual period were selected as study group randomly from July 2011 to November 2011. Another 65 healthy person in our hospital were as control group. ELISA methods was used to detected the iL-10 and TNF-a contents from serum of subjects in the two groups. The serum IL-10 and TNF-a level from two groups and different stages of study group were comparatively analysized. Results Compared with control group, the IL-10 level of vitiligo patients in study group were significantly decreased, and TNF-a were significantly improved. There were significant differences between them( all P < 0. 05 ). The IL-10 of control group and stable stage vitiligo patients were higher than those in advanced stage vitiligo patients ( P < 0. 05) , while the TNF-a of control group and stable stage vitiligo patients were lower than (he advanced stage vitiligo

  16. Vitiligo impact scale: An instrument to assess the psychosocial burden of vitiligo

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    Gaurang S Krishna

    2013-01-01

    Full Text Available Background : Vitiligo is a disease that significantly impairs quality of life. Previous studies have shown that vitiligo has an impact that may not correlate with the size and extent of depigmentation, indicating a need for an independent measure of the psychosocial burden. Aims : To develop a rating scale to assess the psychosocial impact of vitiligo. Methods : The study was undertaken in three broad phases: item generation, pre- and pilot testing, and test administration. Items were generated largely from a qualitative study using semi-structured interviews of patients. Face and content validity were assessed through pre- and pilot testing in 80 patients and the final version was administered to 100 patients who also received the Dermatology Life Quality Index (DLQI and the Skindex-16. Each patient also underwent a physician global assessment (PGA of the impact of vitiligo. Test-retest reliability was assessed in 20 patients. Results: Of 72 items initially generated for the scale, 27 were retained in the final version. Subjects were able to comprehend the items and took about 5-7 min to complete the instrument. The scale was internally consistent (Cronbach′s α = 0.85. Scores on the scale correlated moderately well with the DLQI and the Skindex (Spearman rank correlation: 0.51 and 0.65, respectively. The scale was able to discriminate between patients having mild and those having moderate and severe impact as assessed by PGA. The test-retest reliability coefficient (Spearman rank correlation was 0.80. Conclusion: The Vitiligo Impact Scale appears to be a valid measure of the psychosocial impact of vitiligo and this instrument may be useful both in the clinic and in clinical trials.

  17. Influences of IL-17 cytokine in peripheral blood of patients with vitiligo with NB-UVB therapy%NB-UVB治疗对白癜风患者外周血IL-17表达的影响

    Institute of Scientific and Technical Information of China (English)

    郭洪飞; 马学良; 张厚毅; 王宝林

    2013-01-01

    目的:探讨NB-UVB对白癜风患者外周血IL-17表达的影响及其可能的免疫学机制。方法38例白癜风患者作为实验组,采用NB-UVB进行治疗,20例健康体检者作为对照组,采用酶联免疫吸附法测定NB-UVB治疗前后白癜风患者及健康体检者外周血IL-17的表达水平。结果白癜风患者外周血IL-17表达水平明显高于对照组,差异有统计学意义(P0.05)。结论 NB-UVB可能通过影响白癜风患者外周血中IL-17的表达来发挥治疗作用。%Objective To study the level of IL-17 in peripheral blood of patients with vitiligo, and to observe the possible mechanism of NB-UVB therapy. Methods The level cytokine in peripheral blood of patients with vitiligo, and to explore the possible immune mechanism of NB-UVB therapy of IL-17 in peripheral blood from 38 vitiligo patients and 20 normal control groups were measured by enzyme linked immunosorbent assay. Results The level of IL-17 in vitiligo patients was higher than the control group (P0.05), but the differences were not signiifcant. Conclusion NB-UVB may inlfuence the level of IL-17 cytokine in peripheral blood of patients with vitiligo to plays a therapeutic role.

  18. NB-UVB 治疗对白癜风患者外周血 IL-23表达的影响%Influences of IL-23 cytokine in peripheral blood of patients with vitiligo with NB-UVB therapy

    Institute of Scientific and Technical Information of China (English)

    郭洪飞; 马学良; 张厚毅; 王宝林

    2013-01-01

    Objective:To study the level of IL-23 in peripheral blood of patients with vitiligo, and to observe the possible mechanism of NB-UVB therapy. Methods:The level cytokine in peripheral blood of patients with vitiligo, and to explore the possible immune mechanism of NB-UVB therapy of IL-23 in peripheral blood from 38 vitiligo patients and 20 normal control groups were measured by enzyme linked immunosorbent assay. Results:The level of IL-23 in vitiligo patients was higher than the control group (P0.05), but the differences were not significant. Conclusion:NB-UVB may influence the level of IL-23 cytokine in peripheral blood of patients with vitiligo to plays a therapeutic role.%目的:探讨 NB-UVB 对白癜风患者外周血 IL-23表达的影响及其可能的免疫学机制。方法:38例白癜风患者作为实验组,采用 NB-UVB 进行治疗,20例健康体检者作为对照组,采用酶联免疫吸附法测定 NB-UVB 治疗前后白癜风患者及健康体检者外周血 IL-23的表达水平。结果:白癜风患者外周血 IL-23表达水平明显高于对照组,差异有统计学意义(P0.05)。结论:NB-UVB 可能通过影响白癜风患者外周血中 IL-23的表达来发挥治疗作用。

  19. Incidence of vitiligo in children with Graves' disease and Hashimoto's thyroiditis

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    Prindaville Brea

    2011-11-01

    Full Text Available Abstract Background Limited data are available on the association between vitiligo and autoimmune thyroid disease in pediatric patients. In addition, reported studies of pediatric patients have been based on a population known to have vitiligo and subsequently evaluated for the presence of thyroid function abnormalities. Methods A retrospective chart review was performed on 333 children who had been followed for thyroid disorders by endocrinologists at the Yale Pediatric Thyroid Center over the last 5 years for autoimmune thyroid disease. Demographical and clinical features of patients found to have thyroid disease and vitiligo were recorded. These studies were approved by the Yale Human Investigation Committee. Results Of the total 333 children and adolescents, 9 (2.7% were noted to have vitiligo. Four patients (44% had Graves' disease and 5 patients (66% had Hashimoto's thyroiditis. For patients with Graves' disease and vitiligo, the average age of onset of thyroid disease was young at 4 ± 0.7 years, and the diagnosis of vitiligo usually preceded that of thyroid disease. For children with Hashimoto's thyroiditis and vitiligo, thyroid disease was diagnosed at an average age of 13.25 ± 2.8 years. Conclusion In our population, 4.6% of children with Graves' disease and 2.0% of children with Hashimoto's thyroiditis had vitiligo. Interestingly, when vitiligo presents with Graves' disease, it occurs in younger rather than older children.

  20. 儿童白癜风患者健康心理培养及护理方法%Training and care of children’s mental health of patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    成爱华; 韩梅海; 韩娴

    2013-01-01

    This paper introduces the main symptoms of vitiligo in children and patients prone to psychological problems, and in clinical work explored the method of psychological health of children with vitiligo culture, recommended that children with vitiligo patients in the medical treatment at the same time also needed good care, appropriate care, especially psychological care for vitiligo rehabilitation played a multiplier effect.%文章介绍了儿童白癜风的主要症状及患者容易产生的心理问题,并在临床工作中探索出儿童白癜风患者健康心理的培养方法,建议儿童白癜风患者在配合医护人员进行治疗的同时也需做好护理,适当的护理,特别是心理护理对白癜风的康复起到事半功倍的效果。

  1. Consanguinity pattern and heritability of Vitiligo in Arar, Saudi Arabia

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    Dhaifallah A Alenizi

    2014-01-01

    Full Text Available Context: Epidemiological studies have shown that vitiligo is a complex trait, involving combinations of pathogenic effects of multiple susceptibility genes as well as environmental risk factors. Aim: To observe whether consanguinity increased the incidence of vitiligo in Saudi patients from Arar. Patients and Methods: This study included 69 Saudi patients with vitiligo and their families. These patients, selected from the experience specialist dermatology center in Arar, from April 2011 to 2012, were interviewed by a dermatologist to confirm the diagnosis and complete a questionnaire. Results: A total of 69 patients, 40 males and 29 females were selected. Their mean age was 34.5 ± 11.8 years with the median age of 23 years. The mean age at onset of disease was 27.9 ± 12.9 years. The mean duration of the disease was 9.7 ± 5.3 years. The frequency of focal, vulgaris, universal, and acrofacial subtypes was 22 (31.9%, 21 (30.4%, 8 (11.6%, and 18 (26.1%, respectively. A positive family history of vitiligo was obtained in 45 (65.2% cases. A comparison of the frequency of vitiligo among siblings in relation to the general population was more in accord with the multifactorial model. Conclusion: Consanguinity in marriage increases the incidence of the disease. Therefore, genetic counseling and premarital examination would be important contributions to lower the prevalence of vitiligo.

  2. Association of giant congenital melanocytic nevus, halo nevus and vitiligo in a 75-year-old patient Associação de nevo melanocítico congênito gigante, nevo halo e vitiligo. Relato de caso em paciente de 75 anos

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    Marina Leite da Silveira

    2012-04-01

    Full Text Available A giant congenital melanocytic nevus represents a rare condition. The halo phenomenon may be seen in congenital or acquired melanocytic nevi. In the literature, association of halo nevus and giant congenital melanocytic nevus is rare and the association of both with vitiligo even more rare. A 75-yearold woman at first consultation complained of a hyperchromic bluish-brown hairy macula on the lower back, buttocks and thighs present since birth and an achromic halo of onset three years ago. The histological features were consistent with congenital melanocytic nevus and halo nevus, respectively. After two years the patient developed achromic areas in normal skin, histologically consistent with vitiligo. The authors emphasize the rarity of this triple combination, the patient's age and the absence of malignant degeneration to date.Nevo melanocitico congênito gigante constitui uma condição rara. O fenômeno halo pode ser observado em nevos melanocíticos congênitos ou adquiridos. Na literatura a associação nevo halo e nevo melanocítico congênito gigante é rara e a associação de ambos com vitiligo ainda mais rara. Mulher de 75 anos que à primeira consulta apresentava mácula hipercrômica castanho-azulada pilosa na região lombar, nádegas e coxas desde o nascimento e halo acrômico de aparecimento há 3 anos. Os histológicos foram compatíveis com nevo melanocítico congênito e nevo halo respectivamente. Após dois anos evoluiu com áreas de acromia à distância, com histológico de vitiligo. Os autores ressaltam a raridade desta tripla associação; a idade da paciente e a ausência de degeneração maligna até o presente momento.

  3. Detection of serum IL-22, IL-6 and TNF-αin patients with vitiligo%白癜风患者血清IL-22、IL-6和TNF-α水平的检测

    Institute of Scientific and Technical Information of China (English)

    霍晶

    2014-01-01

    Objective:To detect the levels of IL-22, IL-6 and TNF-α in serum of patients with vitiligo and investigate the role of them in the onset of vitiligo. Methods:The levels of IL-22, IL-6 and TNF-αwere detected by ELISA in 30 patients with vitiligo and 30 healthy controls. Results: The levels of IL-22, IL-6 and TNF-α in the patients with vitiligo were significantly higher than those in healthy controls (P<0.05). Conclusion:IL-22, IL-6 and TNF-α may be involved in the pathogenesis of vitiligo.%目的::检测白癜风患者血清IL-22、IL-6和TNF-α水平,探讨上述因子在白癜风发病中的可能作用。方法:采用酶联免疫吸附法检测30例白癜风患者和30例正常对照者血清IL-22、IL-6和TNF-α的水平。结果:白癜风患者血清IL-22、IL-6和TNF-α的水平显著高于正常对照组( P<0.05)。结论: IL-22、IL-6和TNF-α可能参与了白癜风的发病过程。

  4. The role of serum vitamin D levels in vitiligo

    Science.gov (United States)

    Karagün, Ebru; Baysak, Sevim; Erden, Gönül; Aktaş, Habibullah; Ekiz, Özlem

    2016-01-01

    Introduction Vitiligo is a common acquired pigmentary skin disorder. Vitamin D is responsible for skin pigmentation, increases tyrosinase activity and melanogenesis, and exhibits immunoregulatory functions. Low levels of vitamin D are associated with many autoimmune diseases, including systemic lupus, diabetes mellitus, rheumatoid arthritis, multiple sclerosis and alopecia areata. Few reports have evaluated serum vitamin D levels in vitiligo patients, and their results are conflicting. Aim To evaluate serum vitamin D levels of vitiligo patients and compare the results with controls. Material and methods In total, 50 vitiligo patients and 47 controls were enrolled in the study. Vitamin D levels were measured from blood samples. Group comparisons were performed using appropriate statistical methods. Results The patients had lower serum vitamin D levels than the controls, but this difference was not significant (p = 0.570). Conclusions It remains unknown whether vitamin D deficiency causes vitiligo. Larger controlled studies are required to prove whether low circulating vitamin D is a causative factor in vitiligo. PMID:27605903

  5. The role of serum vitamin D levels in vitiligo

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    Ebru Karagün

    2016-08-01

    Full Text Available Introduction : Vitiligo is a common acquired pigmentary skin disorder. Vitamin D is responsible for skin pigmentation, increases tyrosinase activity and melanogenesis, and exhibits immunoregulatory functions. Low levels of vitamin D are associated with many autoimmune diseases, including systemic lupus, diabetes mellitus, rheumatoid arthritis, multiple sclerosis and alopecia areata. Few reports have evaluated serum vitamin D levels in vitiligo patients, and their results are conflicting. Aim : To evaluate serum vitamin D levels of vitiligo patients and compare the results with controls. Material and methods : In total, 50 vitiligo patients and 47 controls were enrolled in the study. Vitamin D levels were measured from blood samples. Group comparisons were performed using appropriate statistical methods. Results : The patients had lower serum vitamin D levels than the controls, but this difference was not significant (p = 0.570. Conclusions : It remains unknown whether vitamin D deficiency causes vitiligo. Larger controlled studies are required to prove whether low circulating vitamin D is a causative factor in vitiligo.

  6. The Coexistence of Coeliac Disease, Psoriasis and Vitiligo

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    Sevgi Akarsu

    2010-06-01

    Full Text Available It has been defined that coeliac disease is associated with most of the autoimmune diseases including psoriasis and vitiligo. Here, a 26-year-old woman who was diagnosed palmoplantar pustular psoriasis and already had coeliac disease and vitiligo is reported. According to our opinions, this is the first report describing the development of these three disorders in one patient, even though vitiligo, psoriasis and coeliac disease are common disorders, and the coexistence of the two of them has been previously reported in the literature. This case has been presented to emphasize the importance of considering and inquiring the possible coeliac disease in chronic and autoimmune dermatoses, although psoriasis and vitiligo may have coincidental associations with coeliac disease.

  7. Dermatitis Herpetiformis and Vitiligo

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    Ozlem Karabudak

    2007-11-01

    Full Text Available Dermatitis herpetiformis (DH is a rare immunobullous disorder of the skin that is associated with gluten hypersensitivity. Subepidermal IgA-type antibody deposition against tissue transglutaminase leads to dense neutrophilic microabscess and eventually into vesicles in dermal papillae, which may occasionally merge into bullae. Being a subepidermal vesiculobullous disorder, DH is frequently associated with postinflammatory pigmentary changes, particularly hypopigmentation. However, the association of DH with true vitiligo is extremely rare. Here, we report a 21-year-old male with vitiligo and comorbid DH, and review the literature. This new case had severely pruritic, papular and papulovesicular lesions that were localized symmetrically and partly confined to the pre-existing vitiliginous areas. The skin biopsy specimen taken from an erythematous papule on the elbow showed characteristic findings of DH and vitiligo. Direct immunofluorescence microscopy of the perilesional skin revealed granular IgA deposition of dermal papillae. There are only 10 reports in the literature of DH and vitiligo comorbidity.

  8. Research on serum thyroid autoantibodies levels in patients with vitiligo%白癜风患者血清甲状腺自身抗体水平研究

    Institute of Scientific and Technical Information of China (English)

    贾名妍; 苗青; 于伟; 张艺杰

    2013-01-01

    Objective To measure the level of the serum thyroid autoantibodies in patients with vitiligo and investigate the relationship between vitiligo and autoimmune thyroid diseases.Methods 40 patients with vitiligo were enrolled and 38 age-and sex-matched healthy individuals served as controls.The levels of TPOAb and TGAb of patients with vitiligo and controls were measured by chemiluminescent immunoassay.Results The positivity of TPOAb and TGAb in the serum of patients with vitiligo was higher than that in controls significantly.The positivity of TPOAb in patients with vitiligo was 25.00%,higher than that in controls (2.63% for TPOAb,P<0.05).The positivity of TGAb in patients with vitiligo was 22.50%,higher than that in controls(5.26% for TGAb,P<0.05).The positivity of TPOAb and TGAb in female patients with vitiligo was higher than that in male patients (P<0.05).The positivity of TPOAb and TGAb in patients with vitiligo in 21~30 years old was higher than that in patients with vitiligo in the other age (P<0.05).In the different clinical classification and clinical stage,there were no statistical differences in the positivity of TPOAb and TGAb in patients with vitiligo (P>0.05).Conclusions The positivity of TPOAb and TGAb in young female patients with vitiligo was high,and there were correlation between vitiligo and autoimmune thyroid diseases.Clinicians should be aware of the risk of the concurrence of vitiligo and autoimmune thyroid disease,and ensure early screening,early detection and early treatment.%目的:检测白癜风患者血清甲状腺自身抗体水平,探讨白癜风与自身免疫性甲状腺疾病的相关性.方法:收集白癜风患者40例,年龄和性别相匹配的38例健康人为对照组.采用化学发光免疫分析法测定白癜风患者和对照组血清抗甲状腺过氧化物酶抗体(TP0Ab)、抗甲状腺球蛋白抗体(TGAb)的水平.结果:白癜风患者血清TP0Ab和TGAb的阳性率明显高

  9. [Possible role of psychological and environmental factors in the genesis of childhood vitiligo].

    Science.gov (United States)

    Schwartz, Rodrigo; Sepúlveda, Juan Enrique; Quintana, Teresa

    2009-01-01

    The exposure to stressing situations may play a role in the appearance of vitiligo. Patients with the disease have a greater sensitivity to environmental stress and a lower threshold to generate catecholamine mediated responses. To evaluate the temperament and character of patients with vitiligo and explore the relationship of the disease with negative life events and life quality impairment. The study population were 21 patients with vitiligo aged 5 to 12 years, and two control groups (Gl and G2). Gl was composed by 14 healthy siblings of vitiligo patients. G2 was composed by 21 age and gender matched healthy students from two schools in Santiago, Chile. The Junior Temperament and Character Inventory (JTCI), the Qualitative Psychosocial Development Survey (QPDS), the Life Event Checklist (LEC) and the Children's Life Quality index (CDLQI) were applied (LEC only to vitiligo patients). On the temperament dimensions, vitiligo patients scored high on the "harm avoidance" scale in comparison to G2 (13.7 v/s 10.6). Compared with Gl, QPDS showed in vitiligo patients a higher frequency of fear to strangers (71% and 36%, respectively) and a predominant feeling of fear and shyness in response to changes in a close relative (80% and 8%, respectively). There was a negative correlation (protective factor) between the character dimension "self-directedness" and CDLQI score (r =-0.703). In this group of patients, we found a possible relationship between a specific temperament dimension, vitiligo and its impact on life quality.

  10. Heat shock protein-70 expression in vitiligo and its relation to the disease activity

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    Reham William Doss

    2016-01-01

    Full Text Available Background: Vitiligo is a progressive depigmenting disorder characterized by the loss of functional melanocytes from the epidermis. The etiopathogenesis of vitiligo is still unclear. Heat shock proteins (HSPs are prime candidates to connect stress to the skin. HSPs were found to be implicated in autoimmune diseases such as rheumatoid arthritis and other skin disorders as psoriasis. Aim and Objectives: The aim of this study was to map the level of HSP-70 in vitiligo lesions to declare its role in the pathogenesis and activity of vitiligo. Materials and Methods: The study included thirty patients with vitiligo and 30 age- and sex-matched healthy controls. Vitiligo patients were divided as regards to the disease activity into highly active, moderately active, and inactive vitiligo groups. Skin biopsies were taken from the lesional and nonlesional skin of patients and from the normal skin of the controls. HSP-70 messenger RNA (mRNA expression was estimated using quantitative real-time polymerase chain reaction. Results: Our analysis revealed a significantly higher expression of HSP-70 mRNA in lesional skin biopsies from vitiligo patients compared to nonlesional skin biopsies from vitiligo patients (P < 0.001 and compared to skin biopsies from healthy controls (P < 0.001. The level of HSP-70 was not found to be correlated with age, sex, or disease duration. The expression of HSP-70 was correlated with the disease activity and patients with active vitiligo showed higher mean HSP-70 level compared to those with inactive disease. Conclusions: HSP-70 plays a role in the pathogenesis of vitiligo and may enhance the immune response in active disease.

  11. Possible atomoxetine-induced vitiligo: a case report.

    Science.gov (United States)

    Bilgiç, Özlem; Bilgiç, Ayhan

    2015-09-01

    Vitiligo is a chronic skin disease characterized by the appearance of white depigmented lesion due to a loss of melanocytes. The etiopathogenesis of vitiligo is not clear, but according to the neural theory of vitiligo, the direct and indirect effects of monoamine neurotransmitters cause melanocyte destruction and various studies have supported this theory. Many drugs have been related to the development of vitiligo, and the melanocytotoxic effects of the some of these drugs are thought to be related due to their effects on the monoaminergic system. Furthermore, a recent article reported the development of a localized loss of pigmentation after the application of a methylphenidate patch in a patient with attention-deficit/hyperactivity disorder (ADHD). Atomoxetine is an inhibitor of norepinephrine reuptake sites and is a drug that has been used for the treatment of ADHD. Here, we present a school-aged child with ADHD who displayed a vitiligo lesion following the initiation of atomoxetine. We further discuss the possible impact of the ADHD drugs on the development of vitiligo.

  12. Role of oxidative stress and autoimmunity in onset and progression of vitiligo.

    Science.gov (United States)

    Laddha, Naresh C; Dwivedi, Mitesh; Mansuri, Mohmmad Shoab; Singh, Mala; Gani, Amina R; Yeola, Asmita P; Panchal, Vipul N; Khan, Fazal; Dave, Darshana J; Patel, Ananddeep; Madhavan, Shajil E; Gupta, Richa; Marfatia, Zarna; Marfatia, Yogesh S; Begum, Rasheedunnisa

    2014-05-01

    Vitiligo is an acquired depigmentation disorder characterized by the loss of functional melanocytes from the epidermis. Two major theories of vitiligo pathogenesis include autoimmunity and oxidative stress-mediated toxicity in melanocytes. The present study aimed to evaluate both the hypotheses in vitiligo patients and to investigate their role in the disease onset and progression. Antimelanocyte antibody levels and lipid peroxidation (LPO) levels were evaluated in 427 patients and 440 controls; antithyroid peroxidase (TPO) antibody levels were estimated in 102 patients and 72 controls. Patients showed a significant increase in LPO and antimelanocyte antibody levels compared to controls. Antimelanocyte antibody and LPO levels were higher in active vitiligo compared to stable. Only 9.8% of patients showed the presence of anti-TPO antibodies in their circulation. Oxidative stress may be the initial triggering event to precipitate vitiligo in Gujarat population, which is exacerbated by contributing autoimmune factors together with oxidative stress.

  13. Vitiligo iridis and glaucoma: a rare sequelae of small pox.

    Science.gov (United States)

    Kavitha, S; Patel, S R; Mohini, P; Venkatesh, R; Sengupta, S

    2015-10-01

    Vitiligo iridis refers to focal areas of iris atrophy as sequelae of small pox infection. We report a series of patients with unilateral vitiligo iridis, some of whom presented with secondary open-angle glaucoma. Three patients with vitiligo iridis underwent a comprehensive ophthalmic examination including intraocular pressure (IOP) measurement, slit lamp biomicroscopy, gonioscopy, and fundus evaluation. Patients' facial features were also documented and photographed. All patients were in their sixth decade. Two out the three had elevated IOP (52 mm Hg and 36 mm Hg) in the same eye as vitiligo iridis, at initial presentation. Gonioscopy showed patchy iris hyperpigmentation and fundus evaluation showed glaucomatous optic disc changes in the involved eye. One patient responded favourably to topical antiglaucoma medications, whereas the other was taken up for combined phacoemulsification-trabeculectomy with good results. The third patient had normal IOP in the involved eye. All three patients gave a history of small pox in childhood and had pitted facial scars typical of previous small pox infection. Vitiligo iridis may be associated with the secondary glaucoma as a long-term sequelae of small pox. It may be prudent to periodically follow-up such patients for development of raised IOP in the future.

  14. Role of In vivo reflectance confocal microscopy in determining stability in vitiligo: A preliminary study

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    Wei LI

    2013-01-01

    Full Text Available Background: Vitiligo is an acquired pigmentary disorder. In vivo reflectance confocal microscopy (RCM reproducible imaging technique has already been reported to be useful in the diagnosis of other skin diseases. Objective: To define RCM features of vitiligo on different clinical stages. Materials and Methods: A total of 125 patients with a clinical diagnosis of vitiligo were included in this study. After informed consent, lesional skins of those vitiligo patients were characterized by using RCM. Five patients with inflammatory cell infiltration observed at the edge of skin lesions and another 5 patients without inflammatory cell infiltration were selected. Biopsies were performed at same sites of the RCM examination areas for histological and immune-histological analysis. Results: In the active stage of vitiligo, the RCM examination revealed that the bright dermal papillary rings presented at the dermoepidermal junction level in normal skin lost their integrity or totally disappeared, border between vitiligo lesion and normal skin became unclear, and highly refractile cells that referred to infiltrated inflammatory cells could be seen within the papillary dermis at the edge of the lesions. In the stable stage of vitiligo, the RCM showed a complete loss of melanin in lesional skin and a clear border between lesional and normal skin. Conclusion: A simple clinical examination with RCM may reliably and efficiently allow evaluation of the stability status of vitiligo lesions.

  15. Measurement of interleukin-17 and transforming growth factor-β in patients with vitiligo%白癜风患者白介素-17和转化生长因子-β水平的检测

    Institute of Scientific and Technical Information of China (English)

    卢娜; 许爱娥; 周妙妮; 宋秀祖

    2012-01-01

    Objective To assess the relationship of interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) with the development of vitiligo.Methods Enzyme-linked immunosorbent assay (ELISA) was carried out to measure the levels of IL-17 and TGF-β in sera from 120 patients with vitiligo and 60 healthy controls.The correlations of serum IL-17 and TGF-β levels with patients' gender,stage and duration of disease,involved body area and presence of family history were assessed.Results The level of serum IL-17 was significantly higher in patients with active vitiligo than in the controls and patients with stable vitiligo (both P <0.05).With the rise in involved body area,the level of serum IL-17 gradually increased (x2 =12.656,P <0.05).The level of TGF-β in patients with active vitiligo was a little higher than that in the controls and patients with stable vitiligo,with no significant difference between these groups (both P > 0.05).Conclusions The levels of serum IL-17 and TGF-β are somewhat correlated with the activity of vitiligo,and both of them may play a certain role in the pathogenesis of vitiligo.%目的 探讨白介素-17(IL-17)和转化生长因子-B(TGF-β)与白癜风发病之间的关系.方法 双抗体夹心酶联免疫吸附试验( ELISA)检测120例白癜风患者和60例健康对照血清中IL-17和TGF-β水平.分析两种因子与白癜风患者性别、病期、病程、白斑面积、家族史的关系.结果 进展期白癜风患者血清IL-17水平显著高于健康对照组和稳定期患者(P值均<0.05),随着白癜风患者白斑面积增大,IL-17水平逐渐升高(x2=12.656,P< 0.05);而进展期白癜风患者血清TGF-β水平也高于健康对照组和稳定期患者,但差异无统计学意义(P值均> 0.05).结论 白癜风患者血清IL-17和TGF-β水平与病情有一定关系,两者可能在白癜风发病中发挥作用.

  16. Highlights in pathogenesis of vitiligo.

    Science.gov (United States)

    Mohammed, Ghada F; Gomaa, Amal Ha; Al-Dhubaibi, Mohammed Saleh

    2015-03-16

    Vitiligo is a common pigmentary disorder. Many studies across decades and all over the world have attempted to illustrate the pathogenesis behind it; however, the pathogenesis of vitiligo remains elusive. This review article, we present the findings behind the most and updated theories behind this psychologically debilitating and disfiguring disease. The discussion begun with the role of genetic predisposition followed by neural theory first proposed in the 1950s. We highlight the autoimmune hypothesis, followed by the reactive oxygen species model, zinc-α2-glycoprotein deficiency hypothesis, viral theory, intrinsic theory and biochemical, molecular and cellular alterations accounting for loss of functioning melanocytes in vitiligo. Many theories were elaborated to clarify vitiligo pathogenesis. It is a multifactorial disease involving the interplay of several factors. Future research is needed to clarify the interaction of these factors for better understanding of vitiligo pathogenesis and subsequent successful treatment.

  17. 白癜风患者血浆三种神经肽测定及其临床意义%Detection of Some Plasma Neuropeptides and Its Clinical Significance in Patients with Vitiligo

    Institute of Scientific and Technical Information of China (English)

    涂彩霞; 赵达明; 高明阳; 谷玲; 林熙然

    2001-01-01

    Objective To investigate the relationship between beta endorphin (3 - EP), neuropeptide Y (NPY) and calcitonin gene- related peptide (CGRP) and the pathogenesis of vitiligo. Methods Radioimmunoassay (RIA) was employed to detect the plasma levels of three neuropeptides in 40 patients with various types of vitiligo and in 23 normal controls. Results The plasma levels of both 3 - EP and NPY in vitiligo pateints were significantly higher than those in normal controls. The plasma level of NPY in progressive vitiligo was significantly higher than that in stable one. The plasma level of CGRP in generalized and progressive vitiligo was significantly increased when compared with normal controls. Conclusion These findings indicate that the three mentioned neuropeptides may play a role in the pathogenesis of vitiligo.%目的β内啡肽(β-EP)、神经肽Y(NPY)及降钙素基因相关肽(CGRP)是重要的神经递质,同时也可作为免疫调节因子发挥作用。本研究旨在观察这些神经肽是否可能与白癜风的发病有关。方法用放射免疫分析法测定40例白癜风处于进展期或稳定期的不同类型患者血浆3种神经肽浓度,并与23例正常对照作比较。结果寻常型(局限性与泛发性)、节段型、进展期和稳定期白癜风患者血浆β-EP、NPY水平均较正常对照组显著增高;进展期白癜风NPY比稳定期显著增高;泛发性及进展期白癜风血浆CGRP比正常对照组显著增高。结论结果表明上述三种神经肽与白癜风的发病可能存在一定关系,开发神经肽拮抗剂对于白癜风的治疗可能成为一种新的途径。

  18. Expressions of interleukin 17 (IL-17) and RORγt in peripheral blood of patients with vitiligo%白癜风患者外周血白介素17和孤独核受体的表达

    Institute of Scientific and Technical Information of China (English)

    梁津宁; 王秀敏

    2011-01-01

    目的 探讨Thl7细胞在白癜风免疫发病机制中的作用.方法 采用双抗体夹心ELISA法检测白癜风患者进展期、稳定期和正常人外周血血清中白介素17(IL-17)水平;采用实时荧光定量RT-PCR方法检测外周血单一核细胞中孤独核受体(RORγt)mRNA表达水平,并分析其与疾病活动的相关性.结果 进展期与稳定期白癜风外周血中IL-17、RORγt表达水平与正常人对照组相比显著升高(P<0.01).结论 Th17细胞可能与白癜风的发病机制密切相关.%Objective To investigate the roles of Thl7 cells in the immunological pathogenesis of vitiligo. Methods Forty patients with progressive or stable vitiligo and 20 normal human controls were included in this study. Enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of serum IL-17, and SYBR Green I real-time RT-PCR to detect the mRNA expression of RORγt in peripheral blood mononuclear cells (PBMCs) from these patients. The correlation of the above parameters with disease activity was assessed. Results The levels of serum IL-17 and ROR-yt mRNA in PBMCs were significantly higher in patients with progressive and stable vitiligo than in normal human controls (all P< 0.01). Conclusion Thl7 cells may be closely associated with the pathogenesis of vitiligo.

  19. 白癜风患者应用复方补骨脂酊联合胸腺肽治疗的效果分析%Analysis Treatment Effect on Patients With Vitiligo Combined Compound Fructus Psoraleae Tincture With Thymosin

    Institute of Scientific and Technical Information of China (English)

    张翊萍

    2015-01-01

    目的:探讨白癜风患者应用复方补骨脂酊联合胸腺肽治疗的效果。方法选取80例白癜风患者分为联合用药组和单纯用药组,对比两组的治疗效果。结果联合用药组患者治疗效果、治疗后色素积分均优于单纯用药组,P<0.05,差异具有统计学意义。结论复方补骨脂酊联合胸腺肽治疗白癜风效果良好。%Objective To explore the effect on patients with vitiligo combined compound fructus psoraleae tincture with thymosin. Methods Selected 80 patients with vitiligo were divided into combined treatment group and the drug group, compared two groups of treatment effect. Results The combined treatment group treatment effect and pigment integral after treatment was better than the drug group, P<0.05, had difference statistical significance. Conclusion Compound fructus psoraleae tincture combined with thymosin in treatment of vitiligo good effect.

  20. 白癜风患者血浆中氧化与抗氧化相关指标的检测%Assay of Oxidative Stress in Plasma of Patients with Vitiligo

    Institute of Scientific and Technical Information of China (English)

    刘志军; 刘晶; 唐显华

    2011-01-01

    目的 通过对白癜风患者及健康对照者血浆中氧化与抗氧化相关指标--超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、维生素E(VitE)及一氧化氮(NO)表达水平的测定,探讨氧化应激在白癜风发病机制及疾病发展中的作用和意义.方法 选择60例白癜风患者(患者组)和40名健康志愿者(健康对照组)为研究对象,化学法检测血浆中SOD,CAT,GSH-Px的活性及MDA,VE和NO的含量.结果 患者组血浆中MDA含量及SOD活性明显高于健康对照组(P0.05).结论 白癜风患者血浆中存在氧化-抗氧化失衡,白癜风的发病及病情活动与氧化应激可能相关.%Objective To evaluate the oxidative stress in the pathogenesis of vitiligo by examining indicators of oxidative stress in plasma, which include superoxide of dismutase (SOD), catalase( CAT), glutathione peroxidase( GSH-Px), malonaldehyde( MDA), VitE( Vitamin E) and NO( nitric oxide) . Methods Sixty patients with vitiligo and 40 healthy volunteers were included in the study. Plasma levels of SOD, CAT,GSH-Px, MDA, VitE and NO were determined by chemical method. Results Our results revealed a significantly higher level of plasma MDA and SOD, a lower level of GSH-Px in 60 patients with vitiligo compared with the control (P <0. 0l ); Compared with the control, the MDA levels and SOD activities in plasma in the active vitiligo group were significantly increased( P < 0. 0l ), whlile the GSH-Px activities, levels in the active vitiligo group were significantly decreased (r = -0. 337 ,P < 0.01 ); With the increase of the MDA, there was a linear decrease of activities about GSH-Px in plasma and a linear increase of acitivities about SOD in plasma( r =0. 347 ,P <0.01 ). Compared with the control, the CAT, VE and NO levels in plasma in the active and stable vitiligo groups were not increased significantly ( P > 0.05 ). Conclusion Our study shows that oxidative stress is

  1. 小眼畸形相关转录因子转录调控与白癜风临床类型的相关性研究%Correlation Between Microphthalmia-associated Transcription Factor and Clinical Pattern in Vitiligo Patients

    Institute of Scientific and Technical Information of China (English)

    倪亚杰; 王平; 洪为松; 章莉; 郑俊惠

    2013-01-01

    目的 研究小眼畸形相关转录因子(MITF)及其转录调控的酪氨酸酶相关蛋白(TRPs)与白癜风临床类型的相关性.方法 选择非节段型白癜风(NSV)17例和节段型白癜风(SV) 13例,对所有病例均取白斑区、远离白斑正常肤色区的表皮片,首先应用免疫组织化学方法原位检测黑素细胞的MITF及其主要转录调控分子酪氨酸酶(TYR)、酪氨酸酶相关蛋白1(TYRPI)和酪氨酸酶相关蛋白2(TYRP2)的表达情况,并对所有30例远离白斑正常肤色区的表皮黑素细胞进行培养,应用免疫印迹方法检测培养的黑素细胞MITF及TYR、TYRP1和TYRP2的表达情况.结果 (1)原位表达结果:白斑区:所有病例MITF及TYR、TYRP1和TYRP2的表达均为阴性;正常肤色区:SV的MITF表达显著抑制,与NSV及正常人对比均具有统计学差异(P<0.05),NSV的MITF、NSV与SV的TYR、TYRP1和TYRP2表达与正常人对比均没有统计学差异(P>0.05).(2)培养黑素细胞的蛋白表达结果:NSV的TYRP2表达下调,与正常人对比具有统计学差异(P<0.05),而MITF、TYR和TYRP1表达正常;SV的MITF与NSV及正常人对比均具有统计学差异(P<0.05),而TYR、TYRP1和TYRP2正常表达.结论 NSV和SV可能存在不同的MITF转录调控机制.%Objective To analyze the expression of microphthalmia - associated transcription factor ( MITF) , tyrosinase - related proteins (TRPs) and the correlation with clinical pattern in patients with vitiligo. Methods Suction epidermal sheets were taken from le-sional and non - lesional skin from patients of 17 non - segmental vitiligo ( NSV) and 13 segmental vitiligo ( SV) respectively. MITF and its transcriptional molecules tyrosinase (TYR) , tyrosinase - related protein - 1 (TYRP1) , and tyrosinase - related protein - 2 (TYRP2) of melanocytes from lesional and non - lesional skin were detected by immunohistochemistry. MITF, TYR, TYRP1 and TYRP2 of cultured melanocytes from non -lesional vitiligo skin were detected

  2. Photo(chemotherapy for Vitiligo

    Directory of Open Access Journals (Sweden)

    Güzin Özarmağan

    2010-12-01

    Full Text Available Vitiligo is a skin disease having an impact on the quality of life. Phototherapy or photochemotherapy are the first-line treatment choices in generalized vitiligo and are among the most effective treatment modalities either alone or in combination with other therapies. Treatment methods with ultraviolet include oral / topical psoralen plus UVA (PUVA, psoralen plus sunlight (PUVAsol, UVA, broadband UVB, narrowband UVB, excimer laser, monochromatic excimer light and sunlight. This review focuses on the modalities, principals and studies of UV treatment in vitiligo.

  3. Non-recurrence of carbamazepine induced vitiligo after rechallenge with carbamazepine.

    Directory of Open Access Journals (Sweden)

    Masoomeh Saeedloo

    2013-12-01

    Full Text Available Vitiligo is a rare side effect of carbamazepine whose exact mechanism is unknown. The aim of this report is to describe a single case of vitiligo induced by carbamazepine.The case was a patient with Bipolar I disorder whose medications were changed from valproate to carbamazepine and who developed vitiligo after a short while. We followed the case for about four years when he was rechallenged with carbamazepine.When depigmentation occurred, we immediately discontinued carbamazepine after which the depigmented areas improved gradually. About three years later, he received carbamazepine again, but depigmentation did not recur.Carbamazepine-induced vitiligo is not an absolute contraindication for the prescription of carbamazepine if other choices fail to respond or are not tolerated. The case has implications for the mechanism of medication induced vitiligo.

  4. Electron microscopic observation of mitochondrial ultrastructure in melanocytes of perilesional skin from patients with vitiligo%白癜风皮损边缘黑素细胞线粒体超微结构的电镜观察

    Institute of Scientific and Technical Information of China (English)

    李雪; 周梅华; 吴迪; 孙杰; 朱文元; 赵文娥; 鲁严

    2013-01-01

    Objective To evaluate the changes to mitochondrial ultrastructure in melanocytes of perilesional skin from patients with vitiligo.Methods Skin specimens were obtained from the perilesional area (0.5-1 cm distal to vitiligo lesions) of 10 patients with progressive vitiligo and 10 patients with stable vitiligo,as well as from the normal skin of 10 healthy volunteers.The morphology of melanocytes was observed by using transmission electron microscopy (TEM).Besides,stereological parameters of mitochondria,such as volume density (Vv),surface density (Sv) and numerical density (Nv),were measured.Results In melanocytes from the healthy controls,there were a large number of melanosomes with the number of melanosomes per melanocyte being 28.57± 3.21,which were mainly at stage Ⅲ and Ⅳ; mitochondria with normal structure and densely packed cristae were regularly arranged; autophagosomes were seen occasionally.Compared with the melanocytes from healthy controls,there was an obvious decrease in the number of melanosomes (especially stage Ⅲ melanosomes) in melanocytes from the perilesional skin of patients,with the number of melanosomes per melanocyte being 22.00 ± 6.16 (P < 0.05) and 17.43 ± 6.24 (P < 0.05) in patients with progressive vitiligo and stable vitiligo,respectively.TEM also showed disorganized or disrupted mitochondria in various shapes and sizes,most of which were swelling with obscure cristae and vacuolization,in melanocytes from the perilesional skin,and no autophagy was observed.The three stereological parameters were significantly different between the three groups of tissue specimens (all P < 0.05),with the Nv,Vv and Sv of mitochondria being (7.194 ± 1.434) μm-3,(4.8 ± 1.2) %,(2.42 ± 0.86) μ m-1 respectively in melanocytes from the healthy controls,(4.055 ± 0.906) μm-3,(7.4 ± 2.1)%,(3.58 ± 1.15)μm-1 respectively from patients with progressive vitiligo,(5.311 ± 0.873) μm-3,(6.5 ± 1.4)%,(2.82 ± 0.94) μm-1 respectively from

  5. [Unilateral moyamoya disease associated with acrofacial vitiligo in a 13-year-old patient--case report].

    Science.gov (United States)

    Vranjesević, D; Jović, N; Milovanović, D; Djukić, A

    1994-01-01

    Cerebrovascular Moyamoya disease (MMD) is a clinical entity characterized by stenotic or occlusive lesions around the terminal portions of the internal carotid arteries (ICA) and the formation of abnormal vascular networks at the base or convexity. This condition is sporadic, and there are no pathological explanations for this cause. A case with unilateral juvenile MMD is reported. A 13-year-old girl with sudden episode of left limbs weakness, accompanied by transient dysphasia and seizures attack, was admitted to our hospital for further investigation. Computed tomography scanning showed an area of localized brain swelling in the right capsular-thalamic region. Angiographic findings demonstra-ted unilateral involvement. Cerebral panangiography reve-aled a high grade stenosis of the right supraclinoid ICA and a peculiar network (moyamoya phenomenon) formed by dilated basal collateral perforating arteries and produced a cloudy image resembling "a puff of smoke". Left carotid angiogram showed a collateral circulation through the anterior communicant artery and apportioned to the right cerebral hemisphere. The vertebrobasilar system was normal. An ultrasoud Doppler study demonstrated a severely decreased blood flow gradient (0.1 l/min) through the right hypoplastic ICA, while the left common and the left ICA showed remarkable increased blood flow, partly due to abondant collateral vessels. Acquired, non-progressive vitiligo with areas of complete pigment loss appeared on flexor wrists, extensor distal extremities, superior eyelids and especially on joints regions (elbow, knee and small joints of the hands and fret). This association has not been reported previosly.

  6. Childhood vitiligo : Response to methylprednisolone oral minipulse therapy and topical fluticasone combination

    Directory of Open Access Journals (Sweden)

    Majid Imran

    2009-01-01

    Full Text Available Background: Childhood vitiligo is always a challenge to treat, especially when the disease is progressing rapidly in such a patient. Oral minipulse with betamethasone has been tried in childhood vitiligo and also in some other immune mediated skin disorders with good results. Aims: The aim of the present study was to see the overall efficacy of methylprednisolone oral minipulse therapy in combination with topical fluticasone in progressive childhood vitiligo. The combination was tried to achieve a significant amount of repigmentation of vitiligo lesions already present at the initial visit. Materials and Methods: Four hundred children with progressive vitiligo were enrolled for this study and were prescribed oral methylprednisolone on two consecutive days every week in a minipulse form for a period of six months. In addition, the patients were instructed to apply fluticasone ointment topically once a day on their vitiligo lesions. The patients were assessed for the remission achieved as well as the extent of repigmentation of their already existent lesions. Results: More than 90% of patients went into complete remission after the start of the therapy. Moreover, about 65% (two-thirds of patients achieved good to excellent repigmentation of lesions at the end of six months of therapy. The therapy was also well tolerated and the side effects seen were almost negligible. Conclusions: Oral minipulse treatment with methylprednisolone is an effective treatment option for controlling the disease spread in childhood vitiligo and with the addition of topical fluticasone the extent of repigmentation achieved is also quite significant.

  7. Vitiligo: symptoms, pathogenesis and treatment.

    Science.gov (United States)

    Ghafourian, A; Ghafourian, S; Sadeghifard, N; Mohebi, R; Shokoohini, Y; Nezamoleslami, S; Hamat, R A

    2014-01-01

    Vitiligo is an acquired cutaneous disorder of pigmentation, with an incidence of 0.5% to 2% worldwide. There are three major hypotheses for the pathogenesis of vitiligo that are not exclusive of each other: biochemical/cytotoxic, neural and autoimmune. Recent data provide strong evidence supporting an autoimmune pathogenesis of vitiligo. As vitiligo can have a major effect on quality of life, treatment can be considered and should preferably begin early when then disease is active. Current treatment modalities are directed towards stopping progression of the disease and achieving repigmentation. Therapies include corticosteroids, topical immunomodulators, photo(chemo)therapy, surgery, combination therapies and depigmentation of normally pigmented skin. It seems that traditional Chinese medicine could be more effective than the current treatment for vitligo.

  8. Clinical significance on the determinations of serum autoantibodies and thyroid hormone in patients with vitiligo%白癜风患者自身抗体及甲状腺激素检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    吴俊琪; 姚爱萍; 王美燕; 蒋亦秀; 陆晓东

    2015-01-01

    Objective To investigate the levels and clinical significance of serum autoantibodies and thyroid hormone in patients with vitiligo.Methods The anti-nuclear antibody ( ANA ) , anti-nuclear antibody spectrum (ANAS), free triiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroglobulin antibody ( TG-Ab) and thyroid peroxidase antibody ( TPO-Ab) were determined in 123 patients with vitiligo and 60 healthy controls by indirect immunofluorescence, immunoblotting and chemiluminescence.The determination items were statistically compared between vitiligo group and control group.Results The positive rates of ANA, TG-Ab and TPO-Ab in vitiligo group were higher than those in control group(P0.05).FT3 and FT4 were not statistically significant in the 2 groups(P>0.05).TSH in vitiligo group was higher than that in control group (P<0.05).Conclusions The relationship is close between vitiligo and thyroid autoimmune diseases.ANA, TG-Ab, TPO-Ab and TSH determinations can be usually used in vitiligo patients with autoimmune diseases.%目的:探讨白癜风患者血清中自身抗体和甲状腺激素的水平及临床意义。方法分别用间接免疫荧光法、免疫印迹法、化学发光法检测123例白癜风患者和60名正常对照者抗核抗体( ANA )、抗核抗体谱(ANAS)、游离三碘甲状腺原氨酸( FT3)、游离甲状腺素( FT4)、促甲状腺激素( TSH)、甲状腺球蛋白抗体( TG-Ab)和甲状腺过氧化物酶抗体( TPO-Ab),并对白癜风组和正常对照组间的以上检测指标进行比较分析。结果白癜风组ANA、TG-Ab、TPO-Ab阳性率高于正常对照组(P均<0.01), ANAS阳性率2个组间比较差异无统计学意义(P>0.05);FT3、FT4水平2个组间比较差异无统计学意义(P均>0.05),TSH水平白癜风组高于正常对照组(P<0.05)。结论白癜风与甲状腺自身免疫性疾病之间关系密切, ANA、TG-Ab、TPO-Ab

  9. Highlights in pathogenesis of vitiligo

    OpenAIRE

    Ghada F. Mohammed; Gomaa, Amal HA; Al-Dhubaibi, Mohammed Saleh

    2015-01-01

    Vitiligo is a common pigmentary disorder. Many studies across decades and all over the world have attempted to illustrate the pathogenesis behind it; however, the pathogenesis of vitiligo remains elusive. This review article, we present the findings behind the most and updated theories behind this psychologically debilitating and disfiguring disease. The discussion begun with the role of genetic predisposition followed by neural theory first proposed in the 1950s. We highlight the autoimmune ...

  10. Comparative case control study of clinical features and human leukocyte antigen susceptibility between familial and nonfamilial vitiligo

    Directory of Open Access Journals (Sweden)

    Misri Rachita

    2009-01-01

    Full Text Available Background: Various studies worldwide suggest that human leukocyte antigen (HLA region may be involved in the genetic susceptibility of vitiligo but little information is available from India. Aim: To find the HLA associated susceptibility to develop vitiligo in Indian patients and to detect role of HLA in familial vitiligo. Methods: This was a case controlled study which included all patients suffering from vitiligo over a period of one and half years. Clinical details were noted and sera collected from these patients were screened for the presence of HLA class I antibodies. The clinical features and HLA antigens were assessed and comparison was made between patients with familial and nonfamilial vitiligo. Results: Out of 114 patients studied, 84 had family history and 30 had no family history. Patients with family history of vitiligo have higher chances of acquiring vitiligo if first degree relatives are affected compared to if second degree relatives are affected. Family history of vitiligo is associated with an early onset of vitiligo (< 20 years. There was no statistically significant difference in the type, stability, and severity of vitiligo in both the groups. HLA results in both the groups revealed increase in HLA A2, A11, A31, A33, B17, B35, B40, and B44 alleles while HLA A9, B13, and B53 alleles were decreased. Family history was associated with HLA A2, A28, A31, and B44 alleles. Early onset of vitiligo (< 20 years was significantly associated with HLA A2, A11, B17, B35, and B44 alleles. The patients with severe affection (> 10% area showed in significant association with HLA A10 and B8. Conclusion: Family history of vitiligo is associated with an early onset of vitiligo. There is no correlation of family history with the type of vitiligo, stability of lesions, and areas involved. Severity is not associated with family history. Apart from other alleles, alleles A2, and B44 play a significant role in vitiligo in the Indian patients.

  11. Vitiligo on the back and arm (image)

    Science.gov (United States)

    Vitiligo is characterized by patches of depigmented skin. Here, the contrast is seen very clearly. People with ... light skin may not notice small areas of vitiligo. This person is receiving ultraviolet light treatment to ...

  12. Researchers Pinpoint More Genes Linked to Vitiligo

    Science.gov (United States)

    ... 161452.html Researchers Pinpoint More Genes Linked to Vitiligo Genetic clues to this autoimmune disease could lead ... identified more genes linked to the autoimmune disease vitiligo, which causes patches of white skin and hair. ...

  13. Vitiligo and autoantibodies of celiac disease

    OpenAIRE

    Zabihollah Shahmoradi; Jamshid Najafian; Farahnaz Fatemi Naeini; Farinaz Fahimipour

    2013-01-01

    Background: Vitiligo is an acquired, idiopathic disorder characterized by circumscribed depigmented macules and patches. The exact etiology and pathogenesis of vitiligo is not clear. Many theories have been presented regarding this subject among them aautoimmune theory is the most important one. The association of vitiligo with other autoimmune disorders has been reported, but the relationship between vitiligo and celiac disease is controversial. The aim of this study was to study the frequen...

  14. Experimental Study of Changes of Skin Blister Fluid NPY, IL-12, sICAM-1 and GM-CSF Levels in Patients with Vitiligo in Progressive Stage%白癜风进展期患者皮肤疱液中NPY、sICAM-1、IL-12和GM-CSF水平变化的实验研究

    Institute of Scientific and Technical Information of China (English)

    毕鸣晔; 黄海峰

    2011-01-01

    目的:探讨白癜风病患者的发病、进展与免疫学机制的关系.方法:将确诊为进展期白癜风的80例患者分为寻常型(54例),节段型(26例)组.各以白斑处和非白斑处2组的疱液中的研究指标水平进行比较.疱液NPY和GM-CSF采用RIA;IL-12、sICAM-1水平均采用ELISA.结果:本文测定的患者疱液NPY水平显示,寻常型白癜风患者白斑处与非白斑处无显著差异(P>0.05),节段型白癜风患者白斑处与非白斑处比较则呈显著性差异(P0.05).结论:白癜风病患者的发病及病情进展与疱液NPY、IL-12、sICAM-1和GM-CSF四项指标的变化关系密切,其测定有助于了解其病因及病理学机制.%Objective To explore the significance of changes of skin blister fluid NPY, IL-12, sICAM-1 and GM-CSF levels in patients with vitiligo in progressive stage. Methods 80 patients with vitiligo in progressive stage were divided into two groups (vulgar-is vitiligo groups; n = 54, segmental vitiligo groups: n = 26) Their blister fluid levels of NPY and GM-CSF were determined by radioim-munoassay (RIA ) , and IL-12 and sICAM-1 were determined by enzyme immunoassay . Results The levels of skin blister fluid NPY were definitely higher in vitilignous skin than those in non- vitilignous patches in segmental vitiligo groups (P 0. 05) . The levels of skin blister fluid IL-12,sICAM-1 and GM-CSF were all obviously higher in vitilignous skin than that in non- vitilignous patches in vulgaris vitiligo groups (P 0. 05) . Conclusion The changes of skin blister fluid NPY, IL-12, sICAM-1 and GM-CSF levels in vitilignous skin may be closely related to development of difference type vitiligo patients with vitiligo, determination of 4 indexes might be helpful for studying the pathogenesis and clinical diagnosis of vitiligo.

  15. Stability in vitiligo: Is there a perfect way to predict it?

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    Kanika Sahni

    2013-01-01

    Full Text Available Stability is a hard-to-define concept in the setting of vitiligo, but is nonetheless extremely crucial to the planning of treatment regimens and also in prognosticating for the patient. There are several ways to judge stability in vitiligo, which include clinical features and, recently, many biochemical, cytological and ultrastructural correlates of the same. These recent advances help in not only in prognosticating individual patients but also in elucidating some of the mechanisms for the pathogenesis of vitiligo, including melanocytorrhagy and oxidative damage to melanocytes.

  16. In vivo and in vitro evidence for hydrogen peroxide (H2O2) accumulation in the epidermis of patients with vitiligo and its successful removal by a UVB-activated pseudocatalase.

    Science.gov (United States)

    Schallreuter, K U; Moore, J; Wood, J M; Beazley, W D; Gaze, D C; Tobin, D J; Marshall, H S; Panske, A; Panzig, E; Hibberts, N A

    1999-09-01

    To date there is compelling in vitro and in vivo evidence for epidermal H2O2 accumulation in vitiligo. This paper reviews the literature and presents new data on oxidative stress in the epidermal compartment of this disorder. Elevated H2O2 levels can be demonstrated in vivo in patients compared with healthy controls by utilizing Fourier-Transform Raman spectroscopy. H2O2 accumulation is associated with low epidermal catalase levels. So far, four potential sources for epidermal H2O2 generation in vitiligo have been identified: (i) perturbed (6R)-L-erythro 5,6,7,8 tetrahydrobiopterin (6BH4) de novo synthesis/recycling/regulation; (ii) impaired catecholamine synthesis with increased monoamine oxidase A activities; (iii) low glutathione peroxidase activities; and (iv) "oxygen burst" via NADPH oxidase from a cellular infiltrate. H2O2 overproduction can cause inactivation of catalase as well as vacuolation in epidermal melanocytes and keratinocytes. Vacuolation was also observed in vitro in melanocytes established from lesional and nonlesional epidermis of patients (n = 10) but was reversible upon addition of catalase. H2O2 can directly oxidize 6BH4 to 6-biopterin, which is cytotoxic to melanocytes in vitro. Therefore, we substituted the impaired catalase with a "pseudocatalase". Pseudocatalase is a bis-manganese III-EDTA-(HCO3-)2 complex activated by UVB or natural sun. This complex has been used in a pilot study on 33 patients, showing remarkable repigmentation even in long lasting disease. Currently this approach is under worldwide clinical investigation in an open trial. In conclusion, there are several lines of evidence that the entire epidermis of patients with vitiligo is involved in the disease process and that correction of the epidermal redox status is mandatory for repigmentation.

  17. The role of vitamin D in melanogenesis with an emphasis on vitiligo

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    Khalid AlGhamdi

    2013-01-01

    Full Text Available Vitiligo is a common pigmentary disorder caused by the destruction of functional melanocytes. Vitamin D is an essential hormone synthesized in the skin and is responsible for skin pigmentation. Low levels of vitamin D have been observed in vitiligo patients and in patients with other autoimmune diseases. Therefore, the relationship between vitamin D and vitiligo needs to be investigated more thoroughly. We reviewed the literature to date regarding the role of vitamin D in skin pigmentation. Our review revealed that vitamin D deficiency has been identified in many conditions, including premature and dysmature birth, pigmented skin, obesity, advanced age, and malabsorption. Vitamin D increases melanogenesis and the tyrosinase content of cultured human melanocytes by its antiapoptotic effect. However, a few growth-inhibitory effects on melanocytes were also reported. Vitamin D regulates calcium and bone metabolism, controls cell proliferation and differentiation, and exerts immunoregulatory activities. Vitamin D exerts its effect via a nuclear hormone receptor for vitamin D. The topical application of vitamin D increased the number of L-3,4-dihydroxyphenylalanine-positive melanocytes. The topical application of vitamin D yields significant results when used in combination with phototherapy and ultraviolet exposure to treat vitiligo in humans. Vitamin D decreases the expression of various cytokines that cause vitiligo. In conclusion, application of vitamin D might help in preventing destruction of melanocytes thus causing vitiligo and other autoimmune disorders. The association between low vitamin D levels and the occurrence of vitiligo and other forms of autoimmunity is to be further evaluated.

  18. Simvastatin prevents and reverses depigmentation in a mouse model of vitiligo.

    Science.gov (United States)

    Agarwal, Priti; Rashighi, Mehdi; Essien, Kingsley I; Richmond, Jillian M; Randall, Louise; Pazoki-Toroudi, Hamidreza; Hunter, Christopher A; Harris, John E

    2015-04-01

    Vitiligo is a common autoimmune disease of the skin that results in disfiguring white spots. There are no Food and Drug Administration (FDA)-approved treatments, and current treatments are time-consuming, expensive, and of low efficacy. We sought to identify new treatments for vitiligo, and first considered repurposed medications because of the availability of safety data and expedited regulatory approval. We previously reported that the IFN-γ-induced chemokine CXCL10 is expressed in lesional skin from vitiligo patients, and that it is critical for the progression and maintenance of depigmentation in our mouse model of vitiligo. We hypothesized that targeting IFN-γ signaling might be an effective new treatment strategy. Activation of signal transducer and activator of transcription 1 (STAT1) is required for IFN-γ signaling and recent studies revealed that simvastatin, an FDA-approved cholesterol-lowering medication, inhibited STAT1 activation in vitro. Therefore, we hypothesized that simvastatin may be an effective treatment for vitiligo. We found that simvastatin both prevented and reversed depigmentation in our mouse model of vitiligo, and reduced the number of infiltrating autoreactive CD8(+) T cells in the skin. Treatment of melanocyte-specific, CD8(+) T cells in vitro decreased proliferation and IFN-γ production, suggesting additional effects of simvastatin directly on T cells. Based on these data, simvastatin may be a safe, targeted treatment option for patients with vitiligo.

  19. Immunohistochemical expression of aberrant Notch-1 signaling in vitiligo: an implication for pathogenesis.

    Science.gov (United States)

    Seleit, Iman; Bakry, Ola Ahmed; Abdou, Asmaa Gaber; Dawoud, Noha Mohammed

    2014-06-01

    The etiopathogenetic mechanisms leading to pigment loss in vitiligo are not fully understood. Notch signaling is required for development and maintenance of melanocyte lineage and acts as a key component among keratinocyte-melanocyte interactions. The current study aimed to investigate the possible role of Notch signaling and its effect on the whole melanocyte lineage in vitiligo and correlating it with the different clinicopathologic parameters. Using immunohistochemical technique, Notch-1 expression was evaluated in 50 lesional and 20 perilesional biopsies of patients with vitiligo in comparison with 20 normal skin biopsies as a control group. Lesional biopsies were stained with human melanoma black-45 and tyrosinase-related protein-2 to demonstrate the melanocyte lineage. Membranous and/or nuclear expression of Notch-1 was in favor of control and perilesional skin, whereas cytoplasmic expression appeared only in vitiliginous lesions (P vitiligo were associated with mild to moderate Notch-1 intensity, whereas generalized vitiligo was associated with strong intensity of expression (P = .02). In conclusion, Notch-1 signaling is inactivated in vitiligo with consequent loss of epidermal and/or follicular active melanocytes. Aberrant Notch signaling in vitiliginous white hair and acral and segmental vitiligo may be the cause of their treatment resistance.

  20. 普特彼对白癜风患者皮肤组织液 ET-1和 SCF的影响%Influence of Protopic (tacrolimus) on expression of vitiligo skin tissue fluid of patients with ET-1 and SCF

    Institute of Scientific and Technical Information of China (English)

    朴冰; 栗玉珍; 魏烨平; 王政礼; 王洋洋; 崔英; 王丽梅

    2016-01-01

    Objective To investigate Protopic ( tacrolimus) on the expression of vitiligo skin tissue fluid of patients with ET-1 and SCF, and lay the foundation for further clarify the mechanism of Protopic treatment of vitiligo.Methods 30 cases of vitiligo patients before and after treatment with Protopic white and non-white parts of the skin tissue fluid ET-1 and SCF levels by ELISA.Results White skin tissue fluid ET-1 and SCF levels were significantly higher than non-white parts, the difference was statistically significant.Conclusions Protopic can promote keratinocytes secrete ET-1 and SCF, thereby inducing the formation of melanocytes.%目的:探讨普特彼(他克莫司)对白癜风患者皮肤组织液中ET-1和SCF的表达的影响,为进一步明确普特彼治疗白癜风的作用机理奠定基础。方法采用ELISA法检测70例白癜风患者在普特彼治疗前后白斑和非白斑部位皮肤组织液中ET-1和SCF水平。结果白斑皮肤组织液中ET-1和SCF水平明显高于非白斑部位,差异有统计学意义。结论普特彼能促进角质形成细胞分泌ET-1和SCF,从而诱导黑素细胞的形成。

  1. [Experience in introducing the method of placental therapy in vitiligo in Ukraine].

    Science.gov (United States)

    Mal'tsev, V I; Kaliuzhnaia, L D; Gubko, L M

    1995-01-01

    The preparation "melagenin" stimulating reproduction of melanocytes and synthesis of melanotic pigment was isolated from human placenta in Cuba. As many as 366 pediatric patients with vitiligo who ranged from 4 to 15 years old were followed up for 1 year. In 62 cases the area of depigmentation amounted to 71 to 100%. Treatment with melagenin proved to be effective in 83% of vitiligo patients. Indications and contraindications have been determined.

  2. Clinical analysis of thyroglobulin antibody and thyroid peroxidase antibody and their association with vitiligo

    Directory of Open Access Journals (Sweden)

    Yifen Yang

    2014-01-01

    Full Text Available Background: Recently, the abnormal presence of thyroglobulin antibody (TG-Ab and thyroid peroxidase antibody (TPO-Ab has been reported in vitiligo patients, but presence of TG-Ab and TPO-Ab in patients of different ages and gender, and its association with vitiligo and thyroid autoimmunity has rarely been reported. The aim of our research was to determine whether vitiligo was associated with thyroid autoimmunity and figure out its relationship with age and gender. Materials and Methods: We analyzed TG-Ab, TPO-Ab in age and gender matched 87 vitiligo patients and 90 healthy controls, the patients of vitiligo who were positive for the presence of TG-Ab and TPO-Ab were followed up to confirm autoimmune thyroid disease subsequently. Results: Results showed that the frequencies of TG-Ab (23.0%, 20/87 positivity and TPO-AB (24.1%, 21/87 in vitiligo patients were significantly higher than that in healthy controls (P < 0.05. Moreover, The positivity for of TG-Ab and TPO-Ab was higher in 11-20-year age group and 21-40-year age group than that in age matched healthy controls. We found female patients with vitiligo had higher positive frequencies of TG-Ab and TPO-Ab than healthy female controls. (34.1% vs. 8.8% and 34.1% vs. 11.1%, P = 0.000 and P = 0.011. When 20 patients with TG-Ab and TPO-Ab positivity were followed up for three monthes, 14 of them (70% were diagnosed as having autoimmune thyroid disease compared with age-matched healthy controls (16.7%, χ 2 = 5.4, P = 0.02. Conclusion: TG-Ab and TPO-Ab are likely to be found in female teenagers with vitiligo, and are relevant with respect to subsequent development autoimmune thyroid disease.

  3. The utility of dermoscopy in the diagnosis of evolving lesions of vitiligo

    Directory of Open Access Journals (Sweden)

    Sarvesh S Thatte

    2014-01-01

    Full Text Available Background: Early lesions of vitiligo can be confused with various other causes of hypopigmentation and depigmentation. Few workers have utilized dermoscopy for the diagnosis of evolving lesions of vitiligo. Aim: To analyze the dermoscopic findings of evolving lesions in diagnosed cases of vitiligo and to correlate them histopathologically. Methods: Dermoscopy of evolving lesions in 30 diagnosed cases of vitiligo was performed using both polarized light and ultraviolet light. Result: On polarized light examination, the pigmentary network was found to be reduced in 12 (40% of 30 patients, absent in 9 (30%, and reversed in 6 (20% patients; 2 patients (6.7% showed perifollicular hyperpigmentation and 1 (3.3% had perilesional hyperpigmentation. A diffuse white glow was demonstrable in 27 (90% of 30 patients on ultraviolet light examination. Melanocytes were either reduced in number or absent in 12 (40% of 30 patients on histopathology. Conclusion: Pigmentary network changes, and perifollicular and perilesional hyperpigmentation on polarized light examination, and a diffuse white glow on ultraviolet light examination were noted in evolving vitiligo lesions. Histopathological examination was comparatively less reliable. Dermoscopy appears to be better than routine histopathology in the diagnosis of evolving lesions of vitiligo and can obviate the need for a skin biopsy.

  4. Positive pleiotropic effects of HMG-CoA reductase inhibitor on vitiligo

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    Jobin Jean

    2004-05-01

    Full Text Available Abstract Background HMG-CoA reductase inhibitors (statins are commonly used in medicine to control blood lipid disorder. Large clinical trials have demonstrated that statins greatly reduces cardiovascular-related morbidity and mortality in patients with and without coronary artery disease. Also, the use of HMG-CoA reductase inhibitors has been reported to have immunosuppressive effects. Case presentation We describe an unusual case of regression of vitiligo in a patient treated with high dose simvastatin. The relation between simvastatin and regression of vitiligo in this case report may be related to the autoimmune pathophysiology of the disease. Conclusion This unexpected beneficial impact provides another scientific credence to the hypothesis that immune mechanisms play a role in the development of vitiligo and that the use of statins as immuno-modulator could be of use not only for treatment relative to organ transplant but in other pathologies such as vitiligo.

  5. Clinicopathologic Analysis of Stable and Unstable Vitiligo: A Study of 66 Cases.

    Science.gov (United States)

    Yadav, Amit Kumar; Singh, Priyanka; Khunger, Niti

    2016-08-01

    Vitiligo is an acquired skin disorder characterized by milky-white macules and absence of functioning melanocytes. The cornerstone of its management is the correct categorization of a case into its 2 broad types-stable and unstable vitiligo. This distinction is at present based mainly on clinical criteria because the histopathological features are not fully established. This study was thus undertaken to examine histopathological features of vitiligo and to come up with a reliable and systematic approach toward this diagnostic challenge. All patients presenting with clinical features of vitiligo at our institution were included in the study. A 3-mm punch biopsy was taken from 3 sites-lesional, perilesional, and normal skin. Histopathological examination was primarily focused on evaluating 5 histopathological variables-spongiosis, epidermal lymphocytes, basal cell vacuolation, dermal lymphocytes, and melanophages. A total number of 66 patients were included in the study. There were 30 patients in stable and 36 in unstable vitiligo groups. It was observed that all 5 histopathological pattens were associated with unstable vitiligo. All the cases were then scored using a scoring system devised by the authors and the scores obtained were correlated with clinical categorization. It was observed that while there is a definite overlap in histological findings in the 2 groups, adoption of a systematic reporting system brings more consistency and objectivity in the diagnosis. The authors have recommended diagnoses that should be reported for the various scores. This in turn will help us to more reliably and confidently manage these patients.

  6. Genetic Susceptibility to Vitiligo: GWAS Approaches for Identifying Vitiligo Susceptibility Genes and Loci

    OpenAIRE

    Shen, Changbing; Gao, Jing; Sheng, Yujun; Dou, Jinfa; Zhou, Fusheng; Zheng, Xiaodong; Ko, Randy; Tang, Xianfa; Zhu, Caihong; Yin, Xianyong; Sun, Liangdan; Cui, Yong; Zhang, Xuejun

    2016-01-01

    Vitiligo is an autoimmune disease with a strong genetic component, characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Genetic factors are known to play key roles in vitiligo through discoveries in association studies and family studies. Previously, vitiligo susceptibility genes were mainly revealed through linkage analysis and candidate gene studies. Recently, our understanding of the genetic basis of vitiligo has been rapidly advancing through genome-wid...

  7. Genetic Susceptibility to Vitiligo: GWAS Approaches for Identifying Vitiligo Susceptibility Genes and Loci

    OpenAIRE

    Chang eShen; Jing eGao; Yu Jun Sheng; Jinfa eDou; Fusheng eZhou; Xiaodong eZheng; Randy eKo; Xianfa eTang; Caihong Hong Zhu; Xianyong Yong Yin; Liangdan Dan Sun; Yong eCui; Xue Jun Zhang

    2016-01-01

    Vitiligo is an autoimmune disease with a strong genetic component, characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Genetic factors are known to play key roles in vitiligo through discoveries in association and family studies. Previously, vitiligo susceptibility genes were mainly revealed through linkage analysis and candidate gene studies. Our understanding of the genetic basis of vitiligo has been rapidly advancing through genome-wide association stud...

  8. Tumour necrosis factor-α inhibition can stabilize disease in progressive vitiligo.

    Science.gov (United States)

    Webb, K C; Tung, R; Winterfield, L S; Gottlieb, A B; Eby, J M; Henning, S W; Le Poole, I C

    2015-09-01

    Tumour necrosis factor (TNF)-α, a proinflammatory cytokine central to many autoimmune diseases, has been implicated in the depigmentation process in vitiligo. We review its role in vitiligo by exploring its pro- and anti-inflammatory properties and examine the effects of blocking its actions with TNF-α antagonist therapeutics in reports available in the literature. We found that TNF-α inhibition halts disease progression in patients with progressive vitiligo but that, paradoxically, treatment can be associated with de novo vitiligo development in some patients when used for other autoimmune conditions, particularly when using adalimumab and infliximab. These studies reinforce the importance of stating appropriate outcomes measures, as most pilot trials propose to measure repigmentation, whereas halting depigmentation is commonly overlooked as a measure of success. We conclude that TNF-α inhibition has proven useful for patients with progressive vitiligo, where TNF-α inhibition is able to quash cytotoxic T-cell-mediated melanocyte destruction. However, a lingering concern for initiating de novo disease will likely prevent more widespread application of TNF inhibitors to treat vitiligo.

  9. Topical treatment in vitiligo and the potential uses of new drug delivery systems

    Directory of Open Access Journals (Sweden)

    Garg Bhawna

    2010-01-01

    Full Text Available Vitiligo is a psychologically devastating condition. Topical therapy is employed as first-line treatment in localized vitiligo. Currently, several topical agents are available in many forms viz. methoxsalen (solution and cream, trioxsalen (solution, corticosteroids (gel, cream, ointment and solution and calcineurin inhibitors (ointment and cream. Although topical therapy has an important position in vitiligo treatment, side-effects or poor efficacy affect their utility and patient compliance. Novel drug delivery strategies can play a pivotal role in improving the topical delivery of various drugs by enhancing their epidermal localization with a concomitant reduction in their side-effects and improving their effectiveness. The current review emphasizes the potential of various phospholipid based carriers viz. liposomes, transferosomes, ethosomes, lipid emulsions, solid lipid nanoparticles and organogels in optimizing and enhancing the topical delivery of anti-vitiligo agents, whilst reducing the side effects of drugs commonly used in its topical treatment.

  10. Co-occurrence of vitiligo and Becker's nevus: A case report

    Directory of Open Access Journals (Sweden)

    Ayşegül Yalçınkaya İyidal

    2016-12-01

    Full Text Available Vitiligo is an acquired disorder with an unknown etiology in which genetic and non-genetic factors coexist. Melanocytes are destructed in the affected skin areas and clinically depigmented macules and patches appear on the skin. Becker's nevus (BN appears as hyperpigmented macule, patch or verrucous plaques with sharp and irregular margins and often unilateral occurrence and with associated hypertrichosis in various degrees. Although its pathogenesis is unknown, it is suggested to represent a hamartomatous lesion harboring androgen receptors on the lesion. In this report, we present a 19-year-old male patient who developed vitiligo lesions and then BN adjacent to the vitiligo lesion in the right upper back portion of the body ten years after the initial vitiligo lesion.

  11. Recent advances in understanding vitiligo

    Science.gov (United States)

    Manga, Prashiela; Elbuluk, Nada; Orlow, Seth J.

    2016-01-01

    Vitiligo, an acquired depigmentation disorder, manifests as white macules on the skin and can cause significant psychological stress and stigmatization. Recent advances have shed light on key components that drive disease onset and progression as well as therapeutic approaches. Vitiligo can be triggered by stress to the melanin pigment-producing cells of the skin, the melanocytes. The triggers, which range from sunburn to mechanical trauma and chemical exposures, ultimately cause an autoimmune response that targets melanocytes, driving progressive skin depigmentation. The most significant progress in our understanding of disease etiology has been made on three fronts: (1) identifying cellular responses to stress, including antioxidant pathways and the unfolded protein response (UPR), as key players in disease onset, (2) characterizing immune responses that target melanocytes and drive disease progression, and (3) identifying major susceptibility genes. The current model for vitiligo pathogenesis postulates that oxidative stress causes cellular disruptions, including interruption of protein maturation in the endoplasmic reticulum (ER), leading to the activation of the UPR and expression of UPR-regulated chemokines such as interleukin 6 (IL-6) and IL-8. These chemokines recruit immune components to the skin, causing melanocytes to be targeted for destruction. Oxidative stress can further increase melanocyte targeting by promoting antigen presentation. Two key components of the autoimmune response that promote disease progression are the interferon (IFN)-γ/CXCL10 axis and IL-17-mediated responses. Several genome-wide association studies support a role for these pathways, with the antioxidant gene NRF2, UPR gene XBP1, and numerous immune-related genes including class I and class II major histocompatibility genes associated with a risk for developing vitiligo. Novel approaches to promote repigmentation in vitiligo are being investigated and may yield effective, long

  12. Recent advances in understanding vitiligo.

    Science.gov (United States)

    Manga, Prashiela; Elbuluk, Nada; Orlow, Seth J

    2016-01-01

    Vitiligo, an acquired depigmentation disorder, manifests as white macules on the skin and can cause significant psychological stress and stigmatization. Recent advances have shed light on key components that drive disease onset and progression as well as therapeutic approaches. Vitiligo can be triggered by stress to the melanin pigment-producing cells of the skin, the melanocytes. The triggers, which range from sunburn to mechanical trauma and chemical exposures, ultimately cause an autoimmune response that targets melanocytes, driving progressive skin depigmentation. The most significant progress in our understanding of disease etiology has been made on three fronts: (1) identifying cellular responses to stress, including antioxidant pathways and the unfolded protein response (UPR), as key players in disease onset, (2) characterizing immune responses that target melanocytes and drive disease progression, and (3) identifying major susceptibility genes. The current model for vitiligo pathogenesis postulates that oxidative stress causes cellular disruptions, including interruption of protein maturation in the endoplasmic reticulum (ER), leading to the activation of the UPR and expression of UPR-regulated chemokines such as interleukin 6 (IL-6) and IL-8. These chemokines recruit immune components to the skin, causing melanocytes to be targeted for destruction. Oxidative stress can further increase melanocyte targeting by promoting antigen presentation. Two key components of the autoimmune response that promote disease progression are the interferon (IFN)-γ/CXCL10 axis and IL-17-mediated responses. Several genome-wide association studies support a role for these pathways, with the antioxidant gene NRF2, UPR gene XBP1, and numerous immune-related genes including class I and class II major histocompatibility genes associated with a risk for developing vitiligo. Novel approaches to promote repigmentation in vitiligo are being investigated and may yield effective, long

  13. Once-daily application of calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment for repigmentation of facial vitiligo.

    Science.gov (United States)

    Newman, Marissa D; Silverberg, Nanette B

    2011-11-01

    Vitiligo vulgaris is an autoimmune pigmentary disorder with no universally efficacious therapeutic options. Separate applications of calcipotriene ointment 0.005% and topical corticosteroid ointments have been successful in the repigmentation of vitiligo. We sought to examine the efficacy of a combination calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment in the repigmentation of vitiligo. An institutional review board-approved retrospective chart review was conducted in 13 pediatric and adult patients with vitiligo treated with calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment once daily for at least 2 months. Two of 3 children had 76% to 100% repigmentation of facial vitiligo with once-daily usage after 2 months. Of the 10 adults (aged 28-55 years), 1 had 100% facial repigmentation in 3 months, 1 had 76% to 99% facial repigmentation in 5 to 9 months, and 2 had 26% to 50% repigmentation in 3 months. Twelve patients developed some facial repigmentation. No patients experienced atrophy, telangiectases, or lesion enlargement during treatment. Combination calcipotriene 0.005%-betamethasone dipropionate 0.064% ointment shows promise as a once-daily vitiligo therapy. Adult and pediatric facial vitiligo patients may see repigmentation as early as 2 months after initiation of therapy. Children may experience a better response, but larger studies are needed.

  14. Suction blister grafting for vitiligo: efficacy and clinical predictive factors.

    Science.gov (United States)

    Gou, Darlene; Currimbhoy, Sharif; Pandya, Amit G

    2015-05-01

    Suction blister epidermal grafting (SBEG) is a well-established treatment modality for vitiligo, but predictive factors for outcomes are not well characterized. To determine the efficacy and predictive variables for response to SBEG in patients with vitiligo. A retrospective single-center review of all cases treated with SBEG was performed. Repigmentation was assessed by 2 independent reviewers by assessing pigment spread of grafts during the postoperative period. Repigmentation rates were then compared with patient demographics and transplant location. A total of 28 patients were enrolled in this study. The total number of grafts was 129, of which 86.8% (112/129) survived. Highest rate of graft survival was seen in patients younger than 20 years (100%) and the lowest in patients older than 40 years (75%-78%). Repigmentation was seen in 68% of patients. The highest degree of pigment spread was on the neck (283%) and face (231%), whereas the hands and feet had the least response (119%). Blister grafting is successful in most patients with vitiligo, with a high graft survival rate; however, the degree of pigment spread is variable and depends on clinical characteristics of the patient and graft site.

  15. Regional variation of and association of US birthplace with vitiligo extent.

    Science.gov (United States)

    Silverberg, Jonathan I; Reja, Mishal; Silverberg, Nanette B

    2014-12-01

    IMPORTANCE Little is known about population-based risk factors and regional differences for vitiligo.OBJECTIVE To determine the impact of place of birth and residence on vitiligo extent.DESIGN, SETTING, AND PARTICIPANTS A prospective questionnaire-based study using an online questionnaire with 2786 adults (72.2%of whom resided in the United States) with a history of physician-diagnosed vitiligo.EXPOSURES Regions of birth and residence.MAIN OUTCOMES AND MEASURES Body surface area (BSA) of vitiligo lesions.RESULTS Patients with vitiligo who were born outside the United States had lower odds of vitiligo-affected BSA greater than 25%, even after controlling for race/ethnicity, sex, and current age (logistic regression; adjusted odds ratio [aOR], 0.57 [95%CI, 0.46-0.60]).Birthplace in all continents was associated with lower odds of affected BSA greater than 25%than was birthplace in North America. Adults born outside the United States had less affected BSA whether they resided inside (aOR, 0.58 [96%CI, 0.41-0.81]) or outside the United States(aOR, 0.60 [95%CI, 0.48-0.76]). Birthplace and residence at latitudes closer to the equator were associated with lower rates of affected BSA greater than 25%(P .002). The prevalence of affected BSA greater than 25%varied greatly by state of residence (range,27.3%in Maryland to 100% in North Dakota, South Dakota, and Wyoming) (global Moran index = 0.37; P vitiligo. These results suggest that previously unrecognized regional environmental risk factors, especially early in life, play an important role in vitiligo. Additional studies are needed to confirm these early findings and identify such risk factors.TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT01401374

  16. 进展期白癜风患者血清催乳素检测分析%Analysis of prolactin level of 205 aggressive-phase vitiligo patients and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    阿依努尔·艾买提; 木巴拉克·派祖拉; 艾克拜尔·安扎尔

    2011-01-01

    Objective To explore the relation between the serum prolactin level and the development of aggressive-phase vitiligo.Methods The peripheral blood serum of 205 aggressive-phase vitiligo patients ( trial group) and 98 healthy volunteer(control group) were assayed by chemiluminescence analysis.Results ( 1 ) The data of the prolactin levels and the overall distribution of sample mean had statistically significant differences between the control group and trial group( P < 0.05 ).The PRL level of the trial group [ ( 275.96 ± 178.51 ) μIU/ml ] was significantly lower than that of the control group [ (282.98 ± 115.39)μIU/ml ] ( P < 0.05 ).(2) The PRL level of the female trial group[ (288.68 ± 198.45) μ IU/ml]was significantly different from the female control group[ (303.82 ± 121.62)μIU/ml] ( P < 0.05),and the PRL level between the male trial group [ (256.54 ± 141.72) μIU/ml] and the male control group [ (236.43 ± 84.67 ) μIU/ml ] had no significant differences ( P > 0.05 ).Conclusion PRL should not directly result in or participate in development of vitiligo.So it could not be a diagnostic tests to measure the stage of vitiligo and the diagnosis basis for vitiligo's syndrome differentiation of chinese-medicine or Uygur-medicine.%目的 探讨血清催乳素(PRL)水平与进展期白癜风发生发展的关系.方法 采用化学发光法测定205例进展期白癜风患者(病例组)的PRL水平,并与健康对照组(98例)比较.结果 (1)对照组与病例组催乳素水平数据样本均数及总体分布差异有统计学意义(P<0.05),并且病例组水平[( 275.96±178.51) μIU/ml]低于对照组[(282.98±115.39) μIU/ml];(2)女性病例组的催乳素水平[(288.68±198.45) μIU/ml]与女性对照组[(303.82±121.62)μIU/ml]差异有统计学意义(P<0.05).男性病例组催乳素水平[ (256.54±141.72) μIU/ml]与男性对照组[(236.43±84.67) μIU/ml]差异则无统计学意义(P>0.05).结论 催乳素可能并非直接参与或导

  17. Chemical peeling with phenol : For the treatment of stable vitiligo and alopecia areata

    Directory of Open Access Journals (Sweden)

    Savant Satish

    1999-01-01

    Full Text Available Chemical peeling with 88% phenol was carried out on 142 sites of stable vitiligo (hairy-126, non hairy-16 and on 69 sites of alopecia areata (AA. After cleansing and defatting, phenol was applied on affected areas till a uniform frost appeared. On healing, all the lesions of vitiligo showed perifollicular pigmentation in hairy areas and perilesional repigmentation in non hairy areas. These were further treated with PUVA/PUVASOL. After the healing, 82.5% of hairy sites and 81.3% of non hairy sites showed repigmentation. In cases of AA, patients developed vellus hair. In AA, 72.5% had good regrowth and 27.5% had poor response. Side effects seen were hypopigmentation (58 AA, hyperpigmentation (11 AA, persistent erythema (42 vitiligo, 28 AA, demarcation lines (4 AA, secondary bacterial infection (2 vitiligo, 5 AA and superficial scarring (2 vitiligo, 7 AA. The wounding action of phenol is useful to repigment the vitiligo patches and for induction of regrwoth of hair in alopecia areata.

  18. Monochromatic excimer light versus combination of topical steroid with vitamin D3 analogue in the treatment of nonsegmental vitiligo: a randomized blinded comparative study.

    Science.gov (United States)

    Abdel Latif, Azmy Ahmed; Ibrahim, Shady Mahmoud Attia

    2015-01-01

    Vitiligo is a difficult disease to treat, socially stigmatizing its patients. Monochromatic excimer light (MEL) was developed for use in dermatology and adapted for the treatment of vitiligo. Comparing the efficacy of MEL versus topical combination therapy of vitamin D3 analogue and steroid in the treatment of nonsegmental vitiligo. Forty-four patients with localized and stable nonsegmental vitiligo participated in the present study. In each patient, two lesions were selected and divided randomly into two groups, group A was treated with daily topical combination of calcipotriol and betamethasone and group B was treated with biweekly sessions of MEL for 3 months. Efficacy based on repigmentation percentages were blindly evaluated by two independent physicians and patient's satisfaction. There was significant improvement in both treatment modalities at the end of the study, but without significant differences in both groups. There was a significant difference between both groups regarding the onset of repigmentation (p-value vitiligo.

  19. CHARACTERISTICS OF CHILDHOOD VITILIGO IN A TERTIARY REFERRAL CENTRE IN BANGALORE

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    Belliappa Pemmanda

    2016-05-01

    Full Text Available BACKGROUND Vitiligo is an acquired depigmentary disorder, where approximately 50% of the cases have the onset of their disease prior to the age of 20 years and 25% prior to the age of 14 years. There is limited data on the clinical characteristics including associated cutaneous and ocular abnormalities in childhood vitiligo. AIMS To evaluate the various clinical characteristics and associated cutaneous and ocular abnormalities of childhood vitiligo. METHODS In a prospective, hospital based study over a period of two years; the epidemiology of childhood vitiligo was studied including associated cutaneous and ocular abnormalities. RESULTS Of the total 122 children studied, majority of them were females (n=75, 61.5%, and the rest males (n=47, 38.5%. The mean age of presentation was 8 years. Progression of lesions was present in 36 children (29.5%. The most common site of initial lesion was head and neck followed by lower limbs, genitalia, trunk and upper limbs. Eight children (6.6% had a history of trauma prior to onset of vitiligo. Eighteen children (14.8% had a family history of vitiligo. The most common type was vitiligo vulgaris seen in 45 children (36.9% followed by segmental type in 33 children (27%. Leukotrichia was seen in 51 children (41.8%, while Koebner phenomenon was observed in 30 children (24.6%. Fifteen children (12.3% had an associated cutaneous disorder. These associated disorders were halo nevi in 6 children (4.9%, alopecia areata in 3 children (2.5%, canities in 2 children (1.6%, and cafe au lait macule, nevus depigmentosus, lichen nitidus, lichen striatus in 1 each (0.8%. Thirty children (24.6% had an associated ocular disorder. These associated disorders were eyelid vitiligo in 26 children (21.3%, depigmented spots in the iris in 2 patients (1.6%, lamellar cataract and persistent papillary membrane in 1 each (0.8%. CONCLUSIONS Childhood vitiligo in Bangalore showed preponderance in females and greater number of children (72

  20. Coexistencia de psoriasis y vitiligo.

    OpenAIRE

    María Isabel Moreno; Luis Hernando Moreno

    2009-01-01

    Se presenta el caso de un paciente de 53 años de edad, con historia de 10 años de evolución de vitiligo y quien posteriormente, sobre estas lesiones, desarrollo psoriasis en placas, manifestándose como un fenómeno isomórfico de Koebner. En la actual recibe tratamiento con fototerapia, luz ultravioleta B de banda estrecha, con resultados satisfactorios especialmente en la psoriasis.

  1. Coexistencia de psoriasis y vitiligo.

    Directory of Open Access Journals (Sweden)

    María Isabel Moreno

    2009-12-01

    Full Text Available Se presenta el caso de un paciente de 53 años de edad, con historia de 10 años de evolución de vitiligo y quien posteriormente, sobre estas lesiones, desarrollo psoriasis en placas, manifestándose como un fenómeno isomórfico de Koebner. En la actual recibe tratamiento con fototerapia, luz ultravioleta B de banda estrecha, con resultados satisfactorios especialmente en la psoriasis.

  2. Therapeutic implications of autoimmune vitiligo T cells

    NARCIS (Netherlands)

    K. Oyarbide-Valencia; J.G. van den Boorn; C.J. Denman; M. Li; J.M. Carlson; C. Hernandez; M.I. Nishimura; P.K. Das; R.M. Luiten; I.C. Le Poole

    2006-01-01

    Vitiligo is an autoimmune disease presenting with progressive loss of skin pigmentation. The disease strikes 1% of the world population, generally during teenage years. The progressive loss of melanocytes from depigmenting vitiligo skin is accompanied by cellular infiltrates containing both CD4+ and

  3. Association of Vitamin D Receptor Apa I Gene Polymorphism and Immunological Abnormality in Patients with Vitiligo%白癜风患者维生素D受体Apa I基因多态性及免疫异常的研究

    Institute of Scientific and Technical Information of China (English)

    孙越; 韩菁; 吴瑞勤; 谢匡成; 朱光斗; 施伟民

    2011-01-01

    Objective To investigate the association between vitamin D receptor (VDR) Apa Ⅰ gene polymorphism and immunological abnormality on vitiligo patients. Methods VDR genotypes of 46 vitiligo patients and 50 healthy controls were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP). We also measured the levels of immunoglobulin IgG, IgM, IgA and complements C3, C4 and T lymphocyte subpopulations CD3+, CD4+ and CD8+ of peripheral blood. Results ①There was significant difference in genotype distribution of VDR-Apa Ⅰ polymorphisms between vitiligo patients, whose frequency of aa is higher(P<0.05), and normal controls. ② Vitiligo patients with Aa, aa had higher level of IgA than control cases, and the C3 measured in aa genotype showed the same trend (P<0.01). ③ Compared with normal controls,the percentage of CD3+ was significantly lower in vitiligo patients with aa genotype (P<0.05). Conclusion It suggests that VDR is a susceptible gene for involving in vitiligo, and aa is the susceptible genotype·for vitiligo patients. Immunological abnormality may exist in vitiligo patients with aa genotype. Our study also indicates that the pathogenesis of vitiligo might have some connection with VDR gene and immunological abnormality.%目的 探讨白癜风维生素D(VDR)受体Apa I基因多态性及其与免疫异常的关系.方法 采用聚合酶链反应和限制性片段长度多态性方法,对46例白癜风患者和50例健康人的VDR Apa I基因多态性进行分析,以及各基因型与外周血免疫球蛋白IgA、IgM、IgG,补体C3、C4以及T细胞亚群CD3+、CD4+、CD8+之间的关系.结果 ①VDR-Apa I位点的aa基因型在白癜风患者中出现的频率高于正常对照组(P<.05).②Aa、aa基因型组白癜风患者血清免疫球蛋白IgA水平显著低于正常对照组(P<0.05与P<0.01),aa基因型患者补体C3水平显著低于正常对照组(P<0.01).③aa基因型组白癜风患者的CD3+T淋巴细

  4. Cytokine level mensuration of IL-22 in serum and skin blister fluid with vitiligo patients%白癜风患者血清及皮损疱液中白细胞介素-22水平测定

    Institute of Scientific and Technical Information of China (English)

    臧运书; 李洋; 纪晓丽

    2011-01-01

    目的 检测白癜风患者血清及皮损疱液中白细胞介素-22(interleukin-22,IL-22)水平,分析处于不同病期及经治疗后不同疗效的白癜风患者血清IL-22水平变化情况,探讨该因子在白癜风发病过程中的可能作用.方法 采用酶联免疫吸附测定法对56例患者、20例健康志愿者血清及15例接受表皮移植患者皮损疱液中IL-22因子水平进行测定,对接受治疗的18例患者血清中该因子水平进行监测,采用SPSS13.0软件进行数据分析.结果 进展期、稳定期、对照组血清中IL-22水平依次降低,且两两组间差异均有统计学意义(P <0.01),接受表皮移植患者皮损区疱液中IL-22含量显著高于供皮区水平,差异有统计学意义(P <0.05).18例疗效较好患者血清中IL-22水平与治疗前比较下降明显,差异有统计学意义(P <0.05).疗效较差患者血清中IL-22水平差异无统计学意义(P >0.05).结论 IL-22可能在白癜风发病及加重过程中起重要作用,IL-22水平变化可能成为预测白癜风患者病情进展及评价疗效的指标之一.%Objective To detect the serum and suction blister fluid IL-22 level in vitiligo patients, to analyze the change of serum IL-22 level in different stages and different therapeutic effects, and to explore the possible role of IL-22 in pathogenesy leading to vitiligo.Methods The serum IL-22 level in 56 patients and 20 healthy control, and those in blister fluid of 15 cases treated by epidermic grafting were examined by the ELISA nethods.The serum IL-22 level in 18 subjects who accepted the whole range combined therapy were monitored.All the data were analyzed by SPSS 13.0.Results The serum IL-22 level of active phase, stable phase and controls degrade gradually, and the interclass difference in both groups were statistically significant ( P< 0.01 ).The IL-22 level of skin lesions blister fluid were significantly higher than those of normal skin in cases treated by epidermic

  5. The psychosocial impact of acne, vitiligo, and psoriasis: a review

    Directory of Open Access Journals (Sweden)

    Nguyen CM

    2016-10-01

    Full Text Available Catherine M Nguyen,1 Kourosh Beroukhim,2 Melissa J Danesh,3 Aline Babikian,4 John Koo,3 Argentina Leon3 1University of California, Irvine School of Medicine, Irvine, 2David Geffen School of Medicine at UCLA, Los Angeles, 3Department of Dermatology, University of California San Francisco School of Medicine, San Francisco, 4Touro University College of Osteopathic Medicine, Vallejo, CA, USA Introduction: Chronic skin conditions have been well reported to affect a patient's quality of life on multiple dimensions, including the psychosocial domain. Psychosocial is defined as the interrelation of social factors with an individual's thoughts and behavior. The assessment of the psychosocial impact of skin disease on a patient can help direct the dermatologists' treatment goals. To evaluate the psychosocial impact of skin disease, we conducted a review of the literature on three skin conditions with onsets at various stages of life: acne, vitiligo, and psoriasis. Methods: A PubMed search was conducted in March 2015 using the terms “psychosocial” AND “acne”, “psychosocial” AND “vitiligo”, and “psychosocial” AND “psoriasis”. The results were limited to articles published in English in the past 5 years studying patients of all ages. Results and their references were evaluated for relevance according to their discussion of psychosocial qualities in their patients and the validity of psychosocial assessments. The search for acne yielded 51 results, and eleven were found to be relevant; vitiligo yielded 30 results with ten found to be relevant; and psoriasis yielded 70 results with seven found to be relevant. Results: According to the articles evaluated, 19.2% of adolescent patients with acne were affected in their personal and social lives. Social phobia was present in 45% of patients with acne compared to 18% of control subjects. Race and sex played a role in self-consciousness and social perceptions of the disease. Vitiligo

  6. 白癜风患者中医体质临床研究%A clinical research on the somatotypes of patients with vitiligo from traditional Chinese medical view

    Institute of Scientific and Technical Information of China (English)

    潘毅; 许灿龙; 张思; 张超; 徐丽梅; 蔡瑞康

    2016-01-01

    ObjectiveTo investigate the characteristics of the constitution of the patients with vitiligo from traditional Chinese medical view, thus to provide epidemiological evidence for traditional Chinese medicine(TCM) treatment based on differential diagnosis of diseases, symptoms and signs, and constitutions.MethodsQuestionnaires were iflled by 296 patients with vitiligo, which designed including the following items: sex, age, course of disease, somatotypes in TCM, tongue demonstrations, pulse tracings, etc. The data were statistically analyzed to summarize the relationship between somatotypes and above factors.ResultsThe constituent ratio of the pathological somatotypes and balanced somatotypes was 82.16% and 17.84% respectively. There was significant difference between the two groups (P<0.01). The difference between the constituent ratio of various somatotypes patients with vitiligo has statistical signiifcance (χ2=99.506, P=0.000). This signiifcant difference also exists in different sex or ages (χ2=25.008,P=0.002,χ2=101.236,P=0.000, respectively) but not in different course of disease (χ2=7.692, P=0.464). There was signiifcant difference between different tongue demonstrations and pulse tracings (body of the tongue:χ2=392.381,P=0.000, coated tongue:χ2=345.646,P=0.000,pulse tracings:χ2=159.986,P=0.000, respectively). Red or carmoisine in color and big or fat in shape were frequently seen in the body of the tongue, so was white and greasy fur in coated tongue. Sunken or wiry pulse with small pulses were most common in vitiligo patients (P<0.01).ConclusionThe balanced constitution is the most common type of TCM somatotypes in vitiligo patients, followed by deifciency of vital energy, yin asthenia and other pathological constitution. The somatotypes of patients with vitiligo do have correlation with sex or ages but not the course of disease.%目的:探讨白癜风患者中医体质特点,为临床辨病、辨证、辨体质结合治疗白癜风提供

  7. Tissue grafts in vitiligo surgery - past, present, and future

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    Khunger Niti

    2009-01-01

    Full Text Available Vitiligo, characterized by depigmented macules is a common disorder with a high psychosocial impact, particularly in darker skins. Surgical methods become important in cases where medical therapy fails to cause repigmentation or in cases of segmental vitiligo where the response to surgery is excellent. The basic principle of surgical treatment is autologous grafting of viable melanocytes from pigmented donor skin to recipient vitiliginous areas. Various grafting methods have been described including tissue grafts and cellular grafts. Stability of the disease is the most important criterion to obtain a successful outcome. Counseling of the patient regarding the outcome is vital before surgery. The technique and followup management of the tissue grafts has been described in detail in this review.

  8. Successful repigmentation of vitiligo after allogeneic bone marrow transplantation for Hodgkin′s lymphoma by autologous noncultured melanocyte-keratinocyte transplantation

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    Huijuan Tang

    2015-01-01

    Full Text Available The treatment of vitiligo is derisory since the pathogenesis of vitiligo is not clear at present. Most conservative treatments are difficult to approach satisfactory therapy. So transplantation is the only way left when the disease becomes insensitive to those conservative treatments. Here we describe an 18-year-old patient who developed vitiligo, which was triggered by graft-versus-host disease after a allogeneic bone marrow transplantation for the treatment of Hodgkin′s lymphoma from his sister. In the following treatment to vitiligo, the patient successfully performed the transplantation of autologous uncultured melanocyte on the premise of poor reaction to other conservative methods. We infer that transplantation can be a treatment of the vitiligo after allogeneic bone marrow transplantation.

  9. Relationship between autophagy of melanocytes in patients with vitiligo and clinical types%白癜风患者皮肤黑素细胞自噬与临床类型的相关性

    Institute of Scientific and Technical Information of China (English)

    聂慧琼; 王平; 张小燕; 丁承; 刘娇; 许爱娥

    2016-01-01

    Objective To evaluate autophagy of melanocytes and its mechanism in patients with vitiligo and to analyze its correlation with clinical types of vitiligo.Methods Nine cases of segmental vitiligo (SV) and 11 cases of generalized vitiligo (GV) were recruited in Hangzhou Third Hospital between May 2014 and June 2015.Six people with healthy skin were recruited as controls.Epidermal melanocytes were obtained from the normal colour skin around the white spot area in SV and GV patients and from foreskin in controls for culture in vitro.Cultures for each group contained negative control and rapamycin (30 nmol/L) sub-groups.The autophagy was observed using transmission electron microscopy (TEM) and immunofluorescence laser scanning confocal microscope (LSCFM).Protein expressions of microtubeassociated protein light chain 3 (LC3 Ⅱ) and microphthalmia-associated transcription factor (MITF),tyrosinase (TYR),tyrosinase related protein (TYRP) 1,and TYRP2 were detected by Western blot.Results (1) Autophagy of melanocytes was observed under TEM and LSCFM in both SV and control groups before rapamycin treatment,but not in GV patients.The expressions of autophagosome and LC3 Ⅱ protein were increased in melanocytes in SV,GV and control groups after autophagy induction (SV group 0.58 ± 0.10 vs 0.37 ±0.06;GV group 0.57 ±0.16 vs 0.22 ±0.08;control group 0.67 ±0.09 vs 0.46 ±0.12),and the autophagy intensity was higher in the GV group compared to the SV group and the control group.(2) Before autophagy induction,the expressions of MITF,TYR,TYRP1,and TYRP2 protein were lower in the GV and SV patients compared with the control group;after autophagy induction,the expressions of MITF,TYR,and TYRP1 statistically significantly increased in melanocytes in all the three groups (all P < 0.05),while TYRP2 protein expression was not significantly changed (all P > 0.05).Conclusions Autophagy of melanocytes may be present in vitiligo and affect the expression of functional molecules

  10. Anatomical segmentations in all forms of vitiligo: A new dimension to the etiopathogenesis

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    Venkata Ratnam Attili

    2016-01-01

    Full Text Available Background: We have reported segmented lesions in acral vitiligo as well as in generalized vitiligo and thereby proposed somatic mosaicism as a predisposing feature in all forms of vitiligo. This study is a further attempt to characterize and understand such segmented lesions by screening a large series of patients. Methods: We searched our electronic archives (from 2002 to 2014 and identified/reviewed the photos of 615 vitiligo patients inclusive of all clinical types. Over 3500 photographs were screened for patterns that were repeatedly seen in two or more patients and a composite picture of these were marked on a body map. Results: Similar unilateral/bilateral segmented lesions were identified among all forms of vitiligo during relatively stable phases of the disease. These appeared to be related to small and large anatomical divisions of the body. In rapidly evolving disease on the trunk, the lesions conformed to Blaschkoid patterns. Several instances of stable mirror image lesions, symmetric incremental progressions and regressions were also recorded. Limitations: These are observations of a retrospective, single-center review which need to be substantiated further in larger prospective studies. Conclusion: Similar unilateral/bilateral segmented patterns delineating major/minor anatomical divisions of the body may indicate a preexisting developmental defect (such as mosaicism.

  11. VDR基因多态性与宁夏回汉族白癜风患者的相关性研究%Investigation on association of VDR gene polymorphism with Hui and Han ethnic vitiligo patients in NingXia

    Institute of Scientific and Technical Information of China (English)

    郭睿; 郝雁杰; 汪京峡; 刘霞; 杨文斌

    2012-01-01

    目的 研究VDR基因FokI、BsmI位点与宁夏地区回汉族白癜风患者遗传易感性的关系.方法 应用PCR-RFLP技术进行了宁夏回汉族白癜风患者的VDR基因FokI、BsmI位点频率分布的检测.结果 FokI位点的基因型和等位基因的分布频率在宁夏回汉族白癜风患者和对照组中均无统计学意义.BsmI位点Bb基因型的频率分布在宁夏回族白癜风组和回族健康对照组有显著性差异(P为0.033) ;而且Bb基因型的频率在宁夏白癜风患者组和对照组组中也有统计学意义(P值为0.036).结论 VDR 基因BsmI (rs:1544410) 位点可能是宁夏地区尤其是回族人群中白癜风发病的保护因子.%Objective To evaluate the assoriation of the polymorphism of VDR gene FokI and BsmI sites and the gr netir susrrptibility of Hui and Han cthnic vitiligo patiants in XingXia. Methods Polymarase chain relation scquancc rc strirtion fragment length polymorphism (PCR RFLP ) was used to analyzc the distribution of allles and genotypes of the Fold .mel BsmT sites of the VDR gene among Hui and Han cthnir vitiligo patients in Xingxin. Results There was no smtistirally signifirant rliffercncc in the frequencic.s of VDR FokI genotypes and alleles between the vitiligo patients and healthy controls in Han and Hui nationality (P>0.05). There was statistically signifirant diffcrenre in the frequcncies of Bb genotypes of VDR BsmT site between Hui ethnir vitiligo paticnts mel Hui ethnir healthy rontrols in XingXia (P - 0. 033) , and also between vitiligo patients mel healthy rontrols in XingXia. (P -0.026). Conclusion The Bsm I ( rs: 1544410) site of the VDR gene was likely the proteet factor in the pathogrncsis of vitiligo among XingXia population, especially Hui ethnir.

  12. Vitiligo na infância: características clínicas e epidemiológicas Childhood vitiligo: clinical and epidemiological characteristics

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    Cláudia Márcia de Resende Silva

    2007-02-01

    with vitiligo seen at the Pediatric Dermatology Outpatient Clinics of the Hospital das Clinicas- Universage Federal de Minas Gerais. The variables sex, age of onset of disease and treatment, affected body surface area, clinical type, site, autoimmune disease association, family history of vitiligo and initial treatment were evaluated. The statistical analysis was performed using simple frequency and means were compared through analysis of variance. RESULTS: Females accounted for 60.3% of the sample. The mean age at onset of disease was 5.7 years and the mean age at onset of treatment was 7 years. The body surface area affected was smaller than 1% in 71.8% and the localized type was detected in 76.7%. The most common site affected was the head. Family history of vitiligo was observed in 30.1% of patients. Hypothyroidism was found in one patient and 11% reported autoimmune diseases in their families. The initial treatment was topical steroids in the majority of patients. CONCLUSIONS: the findings of vitiligo in childhood in this study are basically similar to those reported in other countries.

  13. Development of melanocye-keratinocyte co-culture model for controls and vitiligo to assess regulators of pigmentation and melanocytes

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    Ravinder Kumar

    2012-01-01

    Full Text Available Background: There is a need to develop an in vitro skin models which can be used as alternative system for research and testing pharmacological products in place of laboratory animals. Therefore to study the biology and pathophysiology of pigmentation and vitiligo, reliable in vitro skin pigmentation models are required. Aim: In this study, we used primary cultured melanocytes and keratinocytes to prepare the skin co-culture model in control and vitiligo patients. Methods: The skin grafts were taken from control and patients of vitiligo. In vitro co-culture was prepared after culturing primary melanocytes and keratinocytes. Co- cultures were treated with melanogenic stimulators and inhibitors and after that tyrosinase assay, MTT assay and melanin content assay were performed. Results: Melanocytes and keratinocytes were successfully cultured from control and vitiligo patients and after that co-culture models were prepared. After treatment of co-culture model with melanogenic stimulator we found that tyrosinase activity, cell proliferation and melanin content increased whereas after treatment with melanogenic inhibitor, tyrosinase activity, cell proliferation and melanin content decreased. We also found some differences in the control co-culture model and vitiligo co-culture model. Conclusion: We successfully constructed in vitro co-culture pigmentation model for control and vitiligo patients using primary cultured melanocytes and keratinocytes. The use of primary melanocytes and keratinocytes is more appropriate over the use of transformed cells. The only limitation of these models is that these can be used for screening small numbers of compounds.

  14. Development of melanocye-keratinocyte co-culture model for controls and vitiligo to assess regulators of pigmentation and melanocytes

    OpenAIRE

    2012-01-01

    Background: There is a need to develop an in vitro skin models which can be used as alternative system for research and testing pharmacological products in place of laboratory animals. Therefore to study the biology and pathophysiology of pigmentation and vitiligo, reliable in vitro skin pigmentation models are required. Aim: In this study, we used primary cultured melanocytes and keratinocytes to prepare the skin co-culture model in control and vitiligo patients. Methods: The skin grafts wer...

  15. Analysis of Manganese Superoxide Dismutase and Glutathione Peroxidase 1 Gene Polymorphisms in Vitiligo.

    Science.gov (United States)

    Seçkin, Havva Yıldız; Kalkan, Göknur; Bütün, İlknur; Akbaş, Ali; Baş, Yalçın; Karakuş, Nevin; Benli, İsmail

    2016-08-01

    Vitiligo is a hereditary/acquired progressive pigmentation disorder characterized by discoloration of skin as a result of melanocyte dysfunction. Recent studies have proposed that oxidant/antioxidant status plays an important role in vitiligo pathogenesis because of the toxic effects on melanocytes. In this study, we aimed to investigate possible associations of MnSOD Ala-9Val and GPx1 Pro198Leu polymorphisms with vitiligo with in Turkish population. The study group consists of 57 patients with vitiligo and 69 healthy controls. Genotyping is performed to identify MnSOD Ala-9Val and GPx1 Pro198Leu polymorphisms. The method used for genotyping was based on the PCR amplification and detection of polymorphisms by hybridization probes labeled with fluorescent dyes. Both the genotype and allele frequencies of MnSOD Ala-9Val (p = 0.817 and p = 0.553, respectively) and GPx1 Pro198Leu polymorphisms (p = 0.422 and p = 0.673, respectively) were not significantly different between vitiligo patients and the control group. Although no significant difference was found, this is the first report investigating the possible associations between the MnSOD Ala-9Val and GPx1 Pro198Leu polymorphisms in Turkish population. Further studies with large populations will be able to clarify the association better.

  16. 白癜风患者血清巨噬细胞移动抑制因子和肿瘤坏死因子α的表达%Detection of macrophage migration inhibition factor and tumor necrosis factor-α in the sera of patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    肖汉龙; 陶娟; 刘辉峰; 安湘杰; 杨柳; 涂亚庭

    2012-01-01

    Objective To determine the serum levels of macrophage migration inhibition factor (MIF) and tumor necrosis factor-α (TNF-α) in patients with vitiligo,and to investigate their clinical significance.Methods The serum concentrations of MIF and TNF-α were determined by enzyme linked immunosorbent assay (ELISA) and radioimmunoassay respectively in 66 patients with vitiligo and 30 healthy controls.Results The patients with vitiligo vulgaris showed a significant higher serum level of MIF and TNF-α compared with the healthy controls ((9.56 ± 1.65) vs.(5.18 ± 0.81 ) μg/L,(2.38 ± 0.37) vs.(1.78 ± 0.21 ) μg/L,both P < 0.01 ).There was a positive correlation between the serum level of MIF and TNF-α (r =0.89,P < 0.05).No statistical difference was observed in the serum levels of MIF or TNF-α between the healthy controls and patients with segmental vitiligo (both P > 0.05).The serum level of MIF was significantly higher in patients with progressive vitiligo than in those with stable vitiligo (P < 0.01 ).Conclusions MIF and TNF-α might play a certain role in the pathogenesis of vitiligo,and MIF may be related to the activity of vitiligo vulgaris.%目的 探讨白癜风患者血清中巨噬细胞移动抑制因子(MIF)和肿瘤坏死因子α(TNF-α)水平的变化及临床意义.方法 酶联免疫吸附试验和放射免疫法检测66例白癜风患者和30例健康对照血清MIF和TNF-α的表达.结果 寻常型白癜风患者组和健康对照组血清MIF水平分别为(9.56±1.65) μg/L和(5.18±0.81) μg/L,TNF-α水平分别为(2.38±0.37) μg/L和(1.78±0.21) μg/L,寻常型白癜风患者组均显著高于健康对照组(P值均< 0.01).寻常型白癜风患者血清MIF和TNF-α水平呈正相关(r=0.89,P< 0.05).节段型白癜风患者组血清MIF和TNF-α水平与健康对照组比较,差异均无统计学意义(P>0.05).进展期白癜风患者血清MIF水平显著高于稳定期(P<0.01).结论 MIF和TNF-α可能在白癜风的发病机制

  17. Detection of CD4+/CD8+T Lymphocyte Ratio and CD4+CD25+ Treg in Peripheral Blood of Patients with Sporadic Vitiligo%散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+T细胞的检测

    Institute of Scientific and Technical Information of China (English)

    叶蓉; 马刚; 胡小平; 彭曦

    2012-01-01

    目的 检测散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平,探讨其与散发型白癜风发病的关系.方法 散发型白癜风患者29例,男13例,女16例.通过流式细胞仪对散发型白癜风患者外周血CD4+/CD8+T细胞比值及CD4+CD25+调节性T细胞水平进行检测,并与20例健康人相比较.结果 与健康对照组相比,散发型白癜风患者外周血中CD4+/CD8+T细胞比值的差异无统计学意义(P>0.05),而CD4+CD25+调节性T细胞水平明显减少,差异有统计学意义(P<0.05),但在不同病程的患者中CD4+CD25+调节性T细胞数量的差异无统计学意义(P>0.05).结论 散发型白癜风患者外周血中存在CD4+CD25+调节性T细胞水平下降,可能与散发型白癜风的发生发展有一定关系.%Objective To detect the CD4+/CD8+ T lymphocyte ratio and the CD4+CD25+ Treg level in peripheral blood of patients with sporadic vitiligo, and to investigate its role in the pathogenesis of sporadic vitiligo. Methods Peripheral blood samples were taken from 29 outpatients with sporadic vitiligo, including 13 males and 16 females. The CD4+/CD8+ T lymphocyte ratio and the CD4+CD25+ Treg level was detected in peripheral blood of patients with sporadic vitiligo by flow cytometry, as well as controlled samples from 20 healthy human. Results There was no difference on the ratio of CD4+/CD8+ T lymphocyte between the patients with sporadic vitiligo and healthy people (P>0.05). Compared to the controlled group, the proportion of CD4+CD25+ Treg was significantly lower in sporadic vitiligo patients(P0.05). Conclusion The level of CD4+CD25+ Treg is lower in peripheral blood of sporadic vitiligo patients, which might play a role in the pathogenesis and development of sporadic vitiligo.

  18. Rapid Partial Repigmentation of Vitiligo in a Young Female Adult with a Gluten-Free Diet

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    Birgit N. Khandalavala

    2014-12-01

    Full Text Available Vitiligo is a common pigmentary skin condition with a profound impact. Despite a number of therapeutic modalities, few have been demonstrated to result in significant repigmentation within a brief period of time. Reported dietary interventions are sparse. Following exclusion of gluten in the diet, early and extensive repigmentation of facial lesions were noted in a young female adult of Asian ethnicity with acrofacial vitiligo. The majority of the benefits occurred within the first month and stabilized at 4 months. Previous topical and phototherapy had not been found to be effective. The patient was maintained on the previously prescribed dapsone therapy. Dietary elimination can potentially be a disease-modifying intervention for vitiligo and should be considered even in patients without concomitant celiac disease.

  19. Detection of auto antibodies and transplantation of cultured autologous melanocytes for the treatment of vitiligo

    Science.gov (United States)

    Zhu, Mei-Cai; Ma, Hong-Yu; Zhan, Zhi; Liu, Cheng-Gang; Luo, Wei; Zhao, Guang

    2017-01-01

    The aim of the present study was to establish an immunofluorescence method of antibody detection to identify melanocytes in the serum of vitiligo patients. Furthermore, we aimed to establish a method for the culture and proliferation of autologous pure melanocytes and to observe the effect of their transplantation for the treatment of vitiligo. Suspension of epidermal cells with melanocytes was performed using trypsin digestion of normal epiderm from eyelid operation and melanocytes were selectively cultured and proliferated in serum-free M2 medium. FITC-labeled rabbit anti-human antibody was used to detect the relative fluorescence intensity of the melanocytes. After identification with immunological and biological examinations, the melanocytes were transplanted to depigmented areas of vitiligo. Repigmentation was observed continuously. The results indicated that melanocytes could be selectively proliferated in the medium. Subsequently, pure melanocytes without contamination of fibroblast and keratinocyte were harvested. A total of 34 patients suffering vitiligo for between 3 months and 20 years with depigmented area (between 4 cm2 and 70% of body surface) were divided into 19 cases of developing stage and 15 cases of stable stage, according to the change of depigmentation. A total of 15 developing cases were positive for the antibody against melanocytes, with the positive rate of 79%. The titers of serum was >1:50 in 10 patients at the developing stage, and 5 developing patients were 1:10. Among the 15 stable cases, four were positive, with a positive rate of 27%. Fluorescence of antibody was localized in the cytoplasm of the melanocytes. Autologous melanocytes of vitiligo patients could be selectively proliferated in the medium. Next, pure melanocytes without contamination with fibroblasts and keratinocytes were harvested. A total of 16 vitiligo patients with 28 depigmented areas (2–200 cm2) were treated with transplantation of melanocytes. Repigmentation of

  20. Altered E-Cadherin Levels and Distribution in Melanocytes Precede Clinical Manifestations of Vitiligo.

    Science.gov (United States)

    Wagner, Roselyne Y; Luciani, Flavie; Cario-André, Muriel; Rubod, Alain; Petit, Valérie; Benzekri, Laila; Ezzedine, Khaled; Lepreux, Sébastien; Steingrimsson, Eirikur; Taieb, A; Gauthier, Yvon; Larue, Lionel; Delmas, Véronique

    2015-07-01

    Vitiligo is the most common depigmenting disorder resulting from the loss of melanocytes from the basal epidermal layer. The pathogenesis of the disease is likely multifactorial and involves autoimmune causes, as well as oxidative and mechanical stress. It is important to identify early events in vitiligo to clarify pathogenesis, improve diagnosis, and inform therapy. Here, we show that E-cadherin (Ecad), which mediates the adhesion between melanocytes and keratinocytes in the epidermis, is absent from or discontinuously distributed across melanocyte membranes of vitiligo patients long before clinical lesions appear. This abnormality is associated with the detachment of the melanocytes from the basal to the suprabasal layers in the epidermis. Using human epidermal reconstructed skin and mouse models with normal or defective Ecad expression in melanocytes, we demonstrated that Ecad is required for melanocyte adhesiveness to the basal layer under oxidative and mechanical stress, establishing a link between silent/preclinical, cell-autonomous defects in vitiligo melanocytes and known environmental stressors accelerating disease expression. Our results implicate a primary predisposing skin defect affecting melanocyte adhesiveness that, under stress conditions, leads to disappearance of melanocytes and clinical vitiligo. Melanocyte adhesiveness is thus a potential target for therapy aiming at disease stabilization.

  1. Preferential secretion of inducible HSP70 by vitiligo melanocytes under stress.

    Science.gov (United States)

    Mosenson, Jeffrey A; Flood, Kelsey; Klarquist, Jared; Eby, Jonathan M; Koshoffer, Amy; Boissy, Raymond E; Overbeck, Andreas; Tung, Rebecca C; Le Poole, I Caroline

    2014-03-01

    Inducible HSP70 (HSP70i) chaperones peptides from stressed cells, protecting them from apoptosis. Upon extracellular release, HSP70i serves an adjuvant function, enhancing immune responses to bound peptides. We questioned whether HSP70i differentially protects control and vitiligo melanocytes from stress and subsequent immune responses. We compared expression of HSP70i in skin samples, evaluated the viability of primary vitiligo and control melanocytes exposed to bleaching phenols, and measured secreted HSP70i. We determined whether HSP70i traffics to melanosomes to contact immunogenic proteins by cell fractionation, western blotting, electron microscopy, and confocal microscopy. Viability of vitiligo and control melanocytes was equally affected under stress. However, vitiligo melanocytes secreted increased amounts of HSP70i in response to MBEH, corroborating with aberrant HSP70i expression in patient skin. Intracellular HSP70i colocalized with melanosomes, and more so in response to MBEH in vitiligo melanocytes. Thus, whereas either agent is cytotoxic to melanocytes, MBEH preferentially induces immune responses to melanocytes.

  2. Immunological Parameters Associated With Vitiligo Treatments: A Literature Review Based on Clinical Studies

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    Ana Cláudia Guimarães Abreu

    2015-01-01

    Full Text Available Vitiligo, a depigmentary disorder, caused by the loss of melanocytes, affects approximately 1% of the world population, irrespective of skin type, with a serious psychological impact on the patient quality of life. So far, the origin of vitiligo has not been traced and the pathogenesis is complex, involving the interplay of a multitude of variables. Although there is no treatment that ensures the complete cure of the disorder, there are some pharmacological, phototherapy, and surgical therapies available. A series of variables can affect treatment outcome, such as individual characteristics, emotional issues, type of vitiligo, stability of the lesions, and immunological status. The present literature review identified the main immunological parameters associated with treatments for vitiligo. Cytotoxic CD8+ T lymphocytes are the main cell type involved in treatment success, as fewer cells in skin lesions are associated with better results. Other parameters such as cytokines and regulatory T cells may also be involved. Further clinical scientific studies are needed to elucidate the complex mechanisms underlying vitiligo and its treatments, in order to expand the range of therapeutic approaches for each individual case.

  3. Modified Protein Improves Vitiligo Symptoms in Mice

    Science.gov (United States)

    ... 2013 (historical) Modified Protein Improves Vitiligo Symptoms in Mice Altering a key protein involved in the development ... pigmentation loss associated with the skin disorder in mice, according to recent research funded by the NIH’s ...

  4. Triple combination treatment with fractional CO2 laser plus topical betamethasone solution and narrowband ultraviolet B for refractory vitiligo: a prospective, randomized half-body, comparative study.

    Science.gov (United States)

    Li, Lu; Wu, Yan; Li, Li; Sun, Yan; Qiu, Li; Gao, Xing-Hua; Chen, Hong-Duo

    2015-01-01

    Vitiligo on extremities and/or bony prominences is very resistant to treatment. Twenty-five patients with symmetrical and stable vitiligo on extremities and/or bony prominences were enrolled. The treatment side received fractional carbon dioxide laser followed by topical compound betamethasone solution and narrowband ultraviolet B phototherapy. The control side received laser treatment plus phototherapy. The result of treatment side showed that 44% patients achieved over 50% re-pigmentation and patient satisfaction score was 5.12 ± 3.23, higher than those of control (p vitiligo.

  5. Vitiligo treatment with vitamins, minerals and polyphenol supplementation

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    Jalel Akrem

    2009-01-01

    Full Text Available Background: Mammalian pigmentation results from the synthesis and accumulation of photo protective epidermal melanin. Melanin was formed from the amino acid precursor L-tyrosine within specialized cells, the melanocytes. Oxidative stress has been suggested to be the initial pathogenetic event in melanocyte degeneration with H 2 O 2 accumulation in the epidermis of patients with active disease. Auto immunity has been also suggested as another hypothesis in the pathogenesis of depigmentation disorders. Topical corticosteroids and phototherapy as common treatment modalities have been prescribed in patients with vitiligo. However, they are often not effective and safe (epidermal atrophy. Therefore, research for alternative therapies continues. Aims: To evaluate the beneficial effects of a supplementation with antioxidant vitamins (A, C, E and minerals (zinc, selenium for vitiligo treatment. Methods: Forty experimental autoimmune vitiligo mice C57BL6, aged from 5 to 12 months showing visible signs of induced vitiligo, were sequentially randomized into five parallel groups (8 mice per group. Each group mice was allocated an identical pre coded cage. the first group (SZV received the ED+1,4 g zinc (Zn+0.04 g selenium (Se+vitamins (A 118 UI, C 8,5 mg, E 5,4 UI /kg diet, the second group (PSZV received the ED+1,4 g zinc (Zn+0.04 g selenium (Se+vitamins (A 118 UI, C 8.5 mg, E 5,4 UI/kg diet+Polyphenol orally, the group 3 (PSZ received the ED+green tea decoction prepared from 100 g/l (polyphenol orally+1,4 g Zn+0.04 g Se, the 4 (P received the ED+green tea decoction prepared green tea decoction prepared from 100 g/l, the control group 5(C received the ED++ distilled water. Cure was defined as repigmentation of treated sites. Photographic and optical techniques were used both at the baseline and on weekly basis. Results: By the end of the study, mices showed visible repigmentation. Using the investigator′s global assessment, therapeutic success in terms

  6. Effectiveness and safety of topical tacrolimus in treatment of vitiligo

    Directory of Open Access Journals (Sweden)

    Ghasem Rahmatpour Rokni

    2017-01-01

    Full Text Available Vitiligo is one of the most primitive well-known dermatoid disorders with different suggested therapies. Therefore, this study investigated the efficiency and safety of topical tacrolimus in treatment of patients with vitiligo. This study was a clinical randomized designed study pre- post-test method, has been conducted on thirty cases with vitiligo who have referred to polyclinic and dermatology clinic. Participant′s evaluated and demographic information recorded in designed checklist. In the next stage, the disease activity scored by vitiligo index disease activity system. Photography and depigmentation percent has recorded before treatment and further in 4 th , 8 th , 12 th , 16 th , 20 th , and 24 th weeks. Finally, gathered data compared through SPSS-20 software. The final sample comprised 30 persons including: 12 men (40% and 18 women (60%. The average of patient′s age in this study was 26/13 ΁ 18/20 (2-76-year-old. Eleven persons was ≤15 years old and rest was older than 15. Sixty-six lesions have funded in patients that maximum has accrued on face and neck (37/87% and trunk (21/21%. In addition, minimum of lesions is related to genitalia (9/09%. In the in 4 th , 8 th , 12 th , 16 th weeks, improvement in face and neck had increased significantly, into the past weeks. In the 20 th and 24 th weeks, the improvement has increased although it was not significant enhancement. Also about trunk, in the 4 th week the improvement does not have significant increasing in compare to the past week. In the eighth, 12 th , 16 th , 20 th , and 24 th weeks the improvement has been increased significantly in compare to the past weeks. Although in the case of limbs and genitalia, the improvement was lower. There was no significant difference between male and females and age. Although the improvement was, slow in older persons. Study results, has presented applying topical tacrolimus in vitiligo, particularly in face and neck, could be effective and

  7. Engineering a new mouse model for vitiligo.

    Science.gov (United States)

    Manga, Prashiela; Orlow, Seth J

    2012-07-01

    Although the precise mechanisms that trigger vitiligo remain elusive, autoimmune responses mediate its progression. The development of therapies has been impeded by a paucity of animal models, since mice lack interfollicular melanocytes, the primary targets in vitiligo. In this issue, Harris et al. describe a mouse model in which interfollicular melanocytes are retained by Kit ligand overexpression and an immune response is initiated by transplanting melanocyte-targeting CD8+ T cells.

  8. Comparative histopathology of vitiligo and contact depigmentation

    Directory of Open Access Journals (Sweden)

    Mittal R

    1992-01-01

    Full Text Available Fifteen cases of vitiligo and 12 cases of contact depigmentation were compared histopathologically. Incontinence of melanin in dermis was seen in 33% cases of contact depigmentation and 6.6% cases of vitiligo. Mononuclear infiltrate was more in contact depigmentation and had lichenoid character at places. Vasculitis with perivascular mononuclear infiltrate was seen in all cases of contact depigmentation. It is proposed that chemicals responsible for contact destruction of melanocytes.

  9. Vitiligo following intense pulsed light treatment.

    Science.gov (United States)

    Shin, Jung U; Roh, Mi Ryung; Lee, Ju Hee

    2010-07-01

    Vitiligo is an acquired depigmenting disorder characterized by the progressive loss of melanocytes from the epidermis and epidermal appendages, which results in milky-white macular lesions. Various factors are suspected to affect the induction and progression of vitiligo such as emotional shock, sunburn, pregnancy, physical illness and trauma. The intense pulsed light (IPL) device which mostly affects redness and dyspigmentation has a broad spectrum of emissions of white light with wavelengths between approximately 515 and 1200 nm. Adverse effects such as purpura and pigmentary changes are known to be rare. We present a 41-year-old woman who developed multiple round, hypopigmented macules on both the cheek and mandibular area following the treatment with IPL for lentigines and dyspigmentation. Based on biopsy and Wood's lamp examination, diagnosis as vitiligo was made. She was treated with a 308-nm excimer laser. After 3 months of treatment, almost complete repigmentation was seen but another coin-sized hypopigmented patch was noted after 5 months later. Herein, we report a case of vitiligo which developed after IPL treatment. This is the first case to be reported which vitiligo developed after IPL treatment. Therefore, dermatologists should be aware of unsighted vitiligo lesion before IPL treatment.

  10. Acridone acetic acid, sodium salt, as an agent to stop vitiligo progression: a pilot study.

    Science.gov (United States)

    Korobko, Igor V; Lomonosov, Konstantin M

    2014-01-01

    Vitiligo progression is attributed to immune system malfunctioning, thus immunomodulating compounds might be beneficial in stopping vitiligo progression which is a prerequisite for successful repigmentation. The goal of this study was to assess efficacy of acridone acetic acid, sodium salt (Na-AAA), an immunomodulating compound with favorable safety profile, in stabilizing active vitiligo, and to reveal prognostic factors of treatment outcome. Sixty consecutive patients with progressing nonsegmental vitiligo were treated with 10 i.m. injections of Na-AAA every other day. Disease stability was assessed in 1, 3, 6, and 12 months post-treatment. Statistical analysis was applied to correlate treatment outcome and available clinical parameters. Of the 60 patients treated, vitiligo stopped progression in 44 patients (73.3%). Older age (p = 0.0219), age of 35 and older (p = 0.0189, odds ratio (OR) = 5.2, 95% confidence interval (CI) 1.30-20.84) or age of 40 and older (p = 0.0039, OR = 6.48, 95% CI 1.86-22.61), longer disease duration (p = 0.0234), pre-treatment interleukin-6 level over 2 pg/mL (p = 0.0005, OR = 13.7, 95% CI 2.97-63), and over the reference threshold value 5.9 pg/mL (p = 0.0009, OR = 25.8, 95% CI 2.8-239) as well as presence of other autoimmune diseases (p = 0.038, OR = 7.0, 95% CI 1.14-42.97) were negative prognostic factors of treatment success. In conclusion, acridone acetic acid, sodium salt, emerges as an efficient option for stopping vitiligo progression.

  11. Some psychological characteristics of children and adolescents with vitiligo: Our results

    Directory of Open Access Journals (Sweden)

    Prćić Sonja

    2006-01-01

    Full Text Available Introduction. Numerous studies have characterized patients with chronic skin disease as psychologically vulnerable, mainly due to the fact that their condition affects their social relations and all other aspects of life. The purpose of this work was to determine whether there are significant differences in the level of anxiety, severity of depressive symptoms, and presence of stressful life events between adolescent patients with vitiligo and healthy peers. Material and methods. 33 patients with vitiligo aged 10-15 years, and a control group of 60 healthy subjects of the same age, were included in this prospective study. A clinical examination was performed to determine the clinical types of vitiligo, estimate depressive symptoms using the Birleson Depression Scale, and anxiety was evaluated by the Spielberger's scale (State-Trait Anxiety Inventory. For determination of the frequency of stressful events, the Risk Scale was used. Results. Adolescents with vitiligo did not show more pronounced signs of anxiety or depression than healthy subjects; differences were not apparent in the Risk Scale either, considering stressful events. Discussion and conclusion. The lack of differences between the two examined groups might be due to prepubertal age of the majority of subjects. 63.63% of all children included in this study were in the prepubertal age (10-12 years, which is the period when they are still not focused on their own body and changes to physical appearance. It is possible that early onset of vitiligo is a "protective factor", enabling the child to attain compensatory mechanisms to solve the problem of vitiligo through various interests and aspirations, which do not depend on physical appearance. .

  12. 自制“白癜风搽剂”治疗白癜风的临床观察%Clinical observation on treating vitiligo with the homemade vitiligo liniment

    Institute of Scientific and Technical Information of China (English)

    薛晓东; 宋红霞

    2013-01-01

      目的:评价自制“白癜风搽剂”治疗白癜风的疗效与安全性。方法:40例白癜风患者外涂自制“白癜风搽剂”,3个月为1个疗程,观察1个疗程,记录疗效及不良反应。结果:痊愈率为22.5%,总有效率为80%,治疗过程中未发现任何不良反应。结论:自制“白癜风搽剂”对白癜风有较好的治疗效果。%Objective:To evaluate the therapeutic efficacy and safety of homemade vitiligo liniment on treating vitiligo. Methods:40 patients with vitiligo was treated with homemade vitiligo liniment, 3 months for a course of treatment, observed a course of treatment, Record efficacy and adverse reactions. Results:The cure rate was 22.5%, the total efficiency was 80%, the course of treatment did not find any adverse reactions. The homemade vitiligo liniment had a better therapeutic effect on treating vitiligo.

  13. Current and emerging therapy for the management of vitiligo

    OpenAIRE

    Alicia Cecile Borderé; Jo Lambert; Nanny van Geel

    2009-01-01

    Alicia Cecile Borderé, Jo Lambert, Nanny van GeelUniversity Hospital of Ghent, Department of Dermatology, Ghent, BelgiumAbstract: Vitiligo is an acquired cutaneous disorder of pigmentation, with an incidence of 0.5% to 2% worldwide. There are three major hypotheses for the pathogenesis of vitiligo that are not exclusive of each other: biochemical/cytotoxic, neural and autoimmune. Recent data provide strong evidence supporting an autoimmune pathogenesis of vitiligo. As vitiligo can ...

  14. 白癜风患者血清和皮肤提取液中氧化与抗氧化能力的比较研究%A comparative study of oxidation and antioxidant capacity of skin and serum in patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    曾洲平; 林宝珠; 葛天华; 黎超伟

    2015-01-01

    Objective To evaluate the oxidation and antioxidant capacity of skin and serum in the control and pa ̄tients with advanced and stable vitiligo,explore the role of oxidative stress in the pathogenesis of vitiligo. Methods The activity of T-AOC, SOD, CAT and GSH-Px, and the content of MDA of skin and serum were detecte in 50 healthy volunteers and 50 cases with vitiligo. Results The MDA content of skin and serum in the patients with vitili ̄go was significantly higher than that of control ( P0.05) . Conclusion The oxidation and antioxidant capacity was correlated with the activity of advance Vitiligo.%目的:比较白癜风患者与正常对照组人群、进展期与稳定期白癜风患者皮肤和血清中氧化与抗氧化能力关键指标,探讨氧化应激在白癜风患者中的作用与其机制。方法经被检测人员同意,随机选取50例白癜风患者(标记为观察组其中进展期患者30例,稳定期患者20例)和50名健康志愿者(标记为对照组)。试剂盒检测皮肤和血清中T-AOC、SOD,CAT,GSH-Px的活性及MDA含量。结果观察组患者皮肤提取液和血清中MDA含量明显高于对照组( P0�05)。结论进展期患者的白癜风病情活动与皮肤和血清中氧化与抗氧化能力密切相关。

  15. 150例白癜风患者个性特征的调查分析%Personality investigation and analysis on 150 patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    徐智广; 张峻岭

    2011-01-01

    Objective Probe into thepersonal characteristic of sufferers of vitiligo. Methods Eysenck personnality questionnaire,EPQ,test 150 sufferers aging from 16 to 70years old, and go further comparison with the normal standard in each graph,T digit and individual composition. Results Among male of vitiligo, the digital of EPQ were higher than the normal standard (P:P<0.05,E.N.L:P<0.01). Among female, the digital of P.E were both higher than normal standard (P<0.01).But there was no obvious difference between male and female. Conclusion The sufferers have their own characteristics of outgoing and neurotic, and the male's spirit-illed characteristic is obvious. So I pointed out that the characteristic ofvitiligo is one of the reason of causing the illness and recuring ,and meanwhile I suggest that we should pay more attention to and intervene their psychology when we are treating them medically.%目的:探讨白癜风患者的个性特征.方法:应用艾森克个性问卷(Eysenck personality questionnaire,EPQ)(成人版),对150例16~70岁的白癜风患者进行测试,并进一步与全国常模比较EPQ各量表粗分均值,T值及个性构成.结果:白癜风男性患者中P、E、N、L分值均高于常模(P分值P<0.05,E、N、L分值P<0.01);女性患者中P、E分值均高于常模(P<0.01),但不同性别各组间无显著性差异.结论:白癜风患者具有外向、精神质的个性特征,提示白癜风患者的个性特征可能是疾病发生与复发的原因之一,建议在时白癜风病患者进行药物治疗的同时,应及时予以心理关注和干预.

  16. Inflammatory vitiligo versus hypopigmented mycosis fungoides in a 58-year-old Indian female

    Directory of Open Access Journals (Sweden)

    Luis A Soro

    2013-01-01

    Full Text Available Vitiligo, particularly the rarer inflammatory variant, may be difficult to distinguish from hypopigmented mycosis fungoides (MF clinically. Complicating the distinction is that when biopsies are taken from the periphery of early vitiliginous lesions or from lesions with an inflammatory border (inflammatory vitiligo, a dermal lymphocytic infiltrate, exocytosis, interface dermatitis, and mild spongiosis may be seen, all resembling the findings seen in hypopigmented MF. We present a case demonstrating the difficulty in differentiating between these two diseases and examine some characteristic clinical and histopathological features of each. Often, a conclusive diagnosis cannot be made, necessitating close follow-up of the patient and monitoring for progression of their disease over time.

  17. 白癜风患者外周血单一核细胞Toll样受体7、9mRNA表达的初步研究%Expressions of Toll-like receptor 7 and 9 mRNA in peripheral blood mononuclear cells from patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    唐亚平; 李振洁; 林春生; 黎小东

    2012-01-01

    Objective To detect the expressions of Toll-like receptor (TLR) 7 and 9 mRNA in peripheral blood mononuclear cells (PBMCs) from patients with vitiligo and their significance.Methods Real-time fluorescence-based quantitative reverse transcription-PCR was performed to detect the expressions of TLR7 and TLR9 mRNA in PBMCs from 50 patients with vitiligo and 25 normal human controls.Two-sample t test was conducted by using SPSS 11.5 software to compare the expression difference of TLR7 and TLR9 mRNA between the patients and controls.Results The mRNA expression level (the ratio of the absolute copy number of a target gene to a control gene) of TLR7 and TLR9 in PBMCs were significantly higher in patients with vitiligo than in the normal human controls (0.85 ± 1.90 vs.0.44 ± 1.18,P < 0.05; 0.94 ± 2.25 vs.0.11 ±0.31,P < 0.01 ).No significant difference was observed in the expression level of TLR7 or TLR9 mRNA in PBMCs between patients with stable and active vitiligo,or between patients with localized and generalized vitiligo (both P > 0.05).Conclusions The expression level of TLR7 and TLR9 mRNA is elevated in PBMCs from patients with vitiligo,which may be involved in the pathogenesis of vitiligo.%目的 探讨Toll样受体7、9(TLR7、TLR9)mRNA在白癜风患者外周血单一核细胞(PBMC)中的表达及其意义.方法 应用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测50例白癜风患者及25例正常人对照PBMC中TLR7、TLR9 mRNA的表达.采用SPSS11.5软件进行统计学分析,两样本均数比较采用f检验.结果 白癜风患者PBMC中TLR7 mRNA(目的基因绝对定量校正拷贝数为0.85±1.90)、TLR9 mRNA(0.94±2.25)表达均明显高于对照组(分别为0.44±1.18和0.11±0.31,P<0.05和<0.01);稳定期与进展期、局限型与泛发型白癜风患者TLR7、TLR9 mRNA表达差异均无统计学意义(P值均> 0.05).结论 白癜风患者PBMC中TLR7、TLR9 mRNA的表达均上调,TLR7、TLR9的异常表达可能参与白癜风的发病机制.

  18. The psychosocial impact of acne, vitiligo, and psoriasis: a review

    Science.gov (United States)

    Nguyen, Catherine M; Beroukhim, Kourosh; Danesh, Melissa J; Babikian, Aline; Koo, John; Leon, Argentina

    2016-01-01

    Introduction Chronic skin conditions have been well reported to affect a patient’s quality of life on multiple dimensions, including the psychosocial domain. Psychosocial is defined as the interrelation of social factors with an individual’s thoughts and behavior. The assessment of the psychosocial impact of skin disease on a patient can help direct the dermatologists’ treatment goals. To evaluate the psychosocial impact of skin disease, we conducted a review of the literature on three skin conditions with onsets at various stages of life: acne, vitiligo, and psoriasis. Methods A PubMed search was conducted in March 2015 using the terms “psychosocial” AND “acne”, “psychosocial” AND “vitiligo”, and “psychosocial” AND “psoriasis”. The results were limited to articles published in English in the past 5 years studying patients of all ages. Results and their references were evaluated for relevance according to their discussion of psychosocial qualities in their patients and the validity of psychosocial assessments. The search for acne yielded 51 results, and eleven were found to be relevant; vitiligo yielded 30 results with ten found to be relevant; and psoriasis yielded 70 results with seven found to be relevant. Results According to the articles evaluated, 19.2% of adolescent patients with acne were affected in their personal and social lives. Social phobia was present in 45% of patients with acne compared to 18% of control subjects. Race and sex played a role in self-consciousness and social perceptions of the disease. Vitiligo negatively affected marriage potential and caused relationship problems in >50% of patients. Psoriasis negatively affected multiple domains of life, including work, relationships, and social activities. Anxiety and depression affected not only psoriasis patients but also their cohabitants; up to 88% of cohabitants had an impaired quality of life. Conclusion Though all three skin conditions resulted in an increase

  19. Strict Anatomical Colocalization of Vitiligo and Elastolytic Granulomas

    Directory of Open Access Journals (Sweden)

    N. Merino de Paz

    2010-02-01

    Full Text Available Vitiligo is the most common depigmenting disorder, with a worldwide occurrence of 0.1–2% in the general population. Multiple conditions have been described colocalized in vitiligo patches, like psoriasis or lichen planus. However, actinic granuloma has not been described in association with vitiligo lesions so far.

  20. Relationship between anxiety and serum β-endorphin in patients with vitiligo%白癜风患者焦虑状态与血清β-内啡肽水平的关系

    Institute of Scientific and Technical Information of China (English)

    臧运书; 纪晓丽

    2012-01-01

    Objective To investigate the relationship between anxiety and serum p-endorphin in patients with vitiligo. Methods Eighty patients with developing vitiligo ( case group) and 40 healthy controls ( control group) were selected. ZUNG self-rating anxiety scale ( SAS) was employed to determine the anxiety status, and enzyme linked immunosorbent assay ( ELISA) was adopted to measure the serum p-endorphin levels. Results The SAS scores in case group (50. 85 ± 12. 23) was significantly higher than that in control group (37. 86 ±11. 23) (t = 5. 63, P 6 months and between patients with higher education and those without higher education (P>0.05).The mass concentration of serum p-endorphin in case group was (219. 11 ±30. 18) ng/L, which was significantly higher than that in control group[ ( 162. 16 ± 19. 65) ng/L] ( t = 9. 12, P < 0. 01) . In case group, the mass concentration of serum p-endorphin in patients with anxiety was (241.71 ± 31.76) ng/L, which was significantly higher than that in patients without anxiety [ (197. 91 ± 23. 29) ng/L] (t = 8. 66, P < 0. 01). Conclusion Patients with vitiligo may be affected by anxiety, and the serum p-endorphin levels may be associated with vitiligo and anxiety.%目的 探讨白癜风患者焦虑情绪与血清β-内啡肽水平的关系.方法 选择80例进展期白癜风患者(病例组)和40例健康体检者(对照组)为研究对象,采用ZUNG焦虑自评量表(SAS)进行焦虑情绪评分,应用酶联免疫吸附法(ELISA)测定血清β-内啡肽水平.结果 病例组SAS评分(50.85±12.23)显著高于对照组(37.86±11.23)(t=5.63,P<0.01).病例组焦虑发生率(47.5%,38/80)显著高于对照组(5.0%,2/40) (x2 =21,67,P<0.01).在病例组中,对于SAS评分和焦虑发生率,女性患者高于男性患者,未婚患者高于已婚患者,皮肤损伤发生于暴露部位的患者高于发生于非暴露部位的患者,16 ~24岁的患者高于25岁以上的患者,差异均有统计学意义(P <0.05或P<0.01)

  1. 308nm准分子激光治疗对白癜风患者外周血Th1及Th2细胞因子的影响%Effect of 308nm excimer laser therapy on Th1/Th2 cytokines in peripheral blood of patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    李建英; 刘津民; 曹海育; 张玉红; 刘英权

    2014-01-01

    Objective To study the level of Th1 cytokine(IFN-γ) and Th2 cytokine (IL-4) in peripher-al blood of patients with vitiligo , and to explore the possible immune mechanism of 308 nm excimer laser for vitiligo therapy.Methods 50 cases of vitiligo were treated by 308nm excimer laser therapy , twice a week for 24 times.The level of IFN-γand IL-4 in peripheral blood from 50 vitiligo patients before and after treatment and 20 healthy per-son in control group were measured by enzyme linked immunosorbent assay .Results There was no difference be-tween two groups on the level of IL -4 (P>0.05).The level of IFN-γin vitiligo patients was higher than that in the control group (P0.05). Conclusion Th1 cytokine take advantage of vitiligo patients and involved in the pathogenesis of vitiligo .308nm ex-cimer laser therapy can restore the Th 1/Th2 balance .%目的:研究308 nm准分子激光对白癜风患者外周血Th1型细胞因子干扰素γ( IFN-γ)和Th2型细胞因子白细胞介素-4( IL-4)水平的影响,探讨其可能的作用机制。方法50例白癜风患者均采用308 nm准分子激光治疗,每周2次共治疗24次。采用酶联免疫吸附法( ELISA法)检测50例白癜风患者治疗前、后及20例正常健康者外周血IFN-γ及IL-4的水平,并统计临床疗效。结果治疗组治疗前IFN-γ水平较正常对照组升高,比较差异有统计学意义(P<0.05);治疗后较治疗前明显降低(P<0.05),且与正常对照组比较差异无统计学意义(P>0.05)。治疗组治疗前IL-4水平与正常对照组及本组治疗后比较差异均无统计学意义( P>0.05)。结论白癜风患者体内Th1型细胞因子占优势,308 nm准分子激光可恢复白癜风患者Th1及Th2的平衡状态。

  2. The role of IL-17 in vitiligo: A review.

    Science.gov (United States)

    Singh, Rasnik K; Lee, Kristina M; Vujkovic-Cvijin, Ivan; Ucmak, Derya; Farahnik, Benjamin; Abrouk, Michael; Nakamura, Mio; Zhu, Tian Hao; Bhutani, Tina; Wei, Maria; Liao, Wilson

    2016-04-01

    IL-17 is involved in the pathogenesis of several autoimmune diseases; however its role in vitiligo has not been well defined. Emerging human and mouse studies have demonstrated that systemic, tissue, and cellular levels of IL-17 are elevated in vitiligo. Many studies have also shown significant positive correlations between these levels and disease activity, extent, and severity. Treatments that improve vitiligo, such as ultraviolet B phototherapy, also modulate IL-17 levels. This review synthesizes our current understanding of how IL-17 may influence the pathogenesis of autoimmune vitiligo at the molecular level. This has implications for defining new vitiligo biomarkers and treatments.

  3. Smashed skin grafting or smash grafting - a novel method of vitiligo surgery.

    Science.gov (United States)

    Krishnan, Ajay; Kar, Sumit

    2012-10-01

    Vitiligo is an acquired progressive disorder characterized by destruction of melanocytes in the lesion and the lesional hair follicles. A number of new therapeutic options for vitiligo have become available over the last decade or so both on the medical as well as surgical side. One among them is the smashed skin grafting or simply smash grafting, which is a modification of split-thickness grafting. In this method, the graft undergoes "smashing" before being applied to the recipient site. Though a simple and effective procedure, very few people are doing the procedure either due to lack of awareness or due to lack of published data. Smash skin grafting was performed in 26 patients with vitiligo in Mahatma Gandhi Institute of Medical Sciences, Sewagram, Maharashtra, India. Smashed skin grafting helped to achieve more than 90% repigmentation of the vitiligo patches in our patients. Smashed skin grafting is a simple procedure with fewer side effects, better outcome, and high patient satisfaction or, in simple words, it can be considered as an alternative to various conventional surgical modalities like punch grafting and melanocyte cell culture methods. This article deals with the steps of the procedure, results of the surgery in a small group of patients, and the necessity of increasing the awareness of smash grafting. © 2012 The International Society of Dermatology.

  4. Depigmentation therapy in vitiligo universalis with topical 4-methoxyphenol and the Q-switched ruby laser

    NARCIS (Netherlands)

    Njoo, MD; Vodegel, RM; Westerhof, W

    2000-01-01

    Background: Monobenzylether of hydroquinone is used worldwide to remove residual pigment in patients with vitiligo universalis. Because of the side effects reported with this drug, the use of monobenzylether of hydroquinone has been restricted in The Netherlands. Objective: Our purpose was to evalua

  5. miR-196a-2 rs11614913 polymorphism is associated with vitiligo by affecting heterodimeric molecular complexes of Tyr and Tyrp1.

    Science.gov (United States)

    Cui, T-T; Yi, X-L; Zhang, W-G; Wei, C; Zhou, F-B; Jian, Z; Wang, G; Gao, T-W; Li, C-Y; Li, K

    2015-10-01

    Tyrosinase and tyrosinase-related protein 1 (Tyr-Tyrp1) complex plays a critical role in the synthesis of melanin intermediates, which involves the production of reactive oxygen species (ROS) and contributes to the development of vitiligo. Based on our previous observation that rs11614913 single nucleotide polymorphism (SNP) in miR-196a-2 could affect the risk of vitiligo by influencing Tyrp1, we hypothesized that the same SNP could also regulate the level of Tyr in vitiligo. The aim of this study was to evaluate the potential association between rs11614913 SNP in miR-196a-2 and serum Tyr level in vitiligo and the regulatory role of miR-196a-2 in the expression of Tyr in melanocytes. The serum Tyr level was detected in 116 patients with vitiligo and 116 controls by ELISA plate assay. The expression level of Tyrp1 and Tyr in PIG1(normal melanocyte cell lines) cells was analyzed by western blotting. The ROS level and apoptosis rate in PIG1 cells transfected with si-Tyr or control siRNA were tested by flow cytometry. The results show that the individuals with TT+TC genotypes in miR-196a-2 and higher Tyr level in serum had an increased risk of vitiligo compared with those who had the CC genotype and lower Tyr level (P vitiligo.

  6. Clinical patterns of vitiligo and its associated co morbidities: A prospective controlled cross-sectional study in South India

    Directory of Open Access Journals (Sweden)

    D S Krupa Shankar

    2012-01-01

    Full Text Available Aim: The purpose of this study is to assess the clinical patterns and associations of vitiligo, audiometric functions, and ocular involvement and to correlate the morphology, clinical behaviour and comorbidities associated with vitiligo. Settings and Design: For this prospective and cross-sectional study 80 self-reporting patients in the age group 7-75 years with vitiligo attending the outpatient department of Manipal hospital during the period August 2008 to February 2010 were selected and the data was analysed. Materials and Methods: The patients were subjected to detailed history, clinical examination and investigations [complete blood count (CBC, absolute eosinophil count (AEC, erythrocyte sedimentation rate (ESR, thyroid stimulating hormone (TSH, vitamin B12 estimation, fasting blood sugar (FBS, and post prandial blood sugar (PPBS,antibody titre estimations that is antithyroid peroxidase (ATPA, antithyroglobulin (ATA, antinuclear antibodies (ANA,urine analysis], audiometric evaluation and ophthalmic examination. Statistical Analysis Used: The Fisher exact test has been used to find the significance of study parameters on categorical scale between two or more groups. Results: In the present series of 80 cases, 41 (51.25% were males and 39 (48.75% were females. The male to female ratio was 1.05:1. In our study 20% cases gave definite family history of vitiligo and patients in the age group of 20 - 30 years were the most commonly affected. Generalized vitiligo (31.3% was the most common type followed by segmental (30%, focal (18.8%, acrofacial (8.8%, and mucosal vitiligo (11.3%. In the present study there was a high incidence of autoantibodies (22.5%, vitamin B12 deficiency (30%, hypothyroidism (11.3%, elevated absolute eosinophil count (16.3%, hypoacusis (10% and retinal changes (8.8%. This suggests multisystem autoimmunity in vitiligo.

  7. A comparative study on 100% tca versus 88% phenol for the treatment of vitiligo

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    Neerja Puri

    2012-07-01

    Full Text Available There are various medical and surgical modalities for the treatment of vitiligo. Surgical modalities are used in the patients who fail to respond to medical therapy. We selected thirty patients of stable vitiligo from the department of dermatology for the study. The patients were divided into two groups of 15 patients each. In Group I patients application of 100% TCA was done on the vitiliginous sites and in Group II patients 88% phenol was applied on the affected sites. Comparing the results of repigmentation in both the groups it was seen that marked pigmentation was seen in 66.6% patients in the TCA group and 80% in the Phenol group. Moderate pigmentation was seen in 13.3% patients in both the groups and mild pigmentation was seen in 20% patients in the TCA group and 6.6% in the Phenol group.

  8. Vitiligo

    Science.gov (United States)

    ... skin (topically). This is followed by carefully timed exposure to sunlight or to ultraviolet A (UVA) light that comes from a special lamp. You must minimize exposure to sunlight at other times. Known side effects of oral ...

  9. Vitiligo

    Science.gov (United States)

    ... you trust, whether that's a family member, friend, teacher, counselor, or support group. There are plenty of people who love you just the way you ... Hair, and Nails Body Image and Self-Esteem Skin Biopsy Alopecia: Kayla's Story ...

  10. Vitiligo

    Science.gov (United States)

    ... the body in a symmetrical pattern, like a mirror image. This is the most common type of ... athletes use. Photochemotherapy (also known as PUVA ). PUVA therapy has two steps: first, a medication called psoralen ...

  11. The therapeutic effects of EGCG on vitiligo.

    Science.gov (United States)

    Zhu, Yiping; Wang, Suiquan; Lin, Fuquan; Li, Qing; Xu, Aie

    2014-12-01

    Epigallocatechin-3-gallate (EGCG) is one of the main chemical constituents of green tea, which has been used as an important traditional Chinese medicine. Green tea has anti-inflammatory, anti-oxidant, and immunomodulatory properties. However, the effects of EGCG on vitiligo are not known. We assessed the role of EGCG in vitiligo induced by monobenzone in mice. We demonstrated that EGCG: delayed the time of depigmentation; reduced the prevalence of depigmentation; and decreased the area of depigmentation. Examination of depigmented skin treated with EGCG by reflectance confocal microscopy suggested increased numbers of epidermal melanocytes and histologic examination showed decreased perilesional accumulation of CD8(+) T cells. To further investigate the mechanism of the anti-inflammatory effects of EGCG, levels of inflammatory mediator tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-6 were tested by enzyme-linked immunosorbent assay. Serum cytokine levels were significantly decreased after administration of EGCG compared with the model group. These results suggested that EGCG may have protective effects against vitiligo, and that it could contribute to suppression of activation of CD8(+) T cells and inflammatory mediators. Based on these results, 5% EGCG was considered to be the most suitable concentration for treating vitiligo, and was used for further study. In addition, we investigated the gene-expression profile of this model in relation to EGCG. Using a 4×44K whole genome oligo microarray assay, 1264 down-regulated genes and 1332 up-regulated genes were recorded in the 5% EGCG group compared with the model group, and selected genes were validated by real-time polymerase chain reaction. Our study demonstrated that EGCG administration was significantly associated with a decreased risk of vitiligo. EGCG could be a new preventive agent against vitiligo in the clinical setting.

  12. Possible mechanisms in the induction of vitiligo-like hypopigmentation by topical imiquimod.

    Science.gov (United States)

    Mashiah, J; Brenner, S

    2008-01-01

    The pathogenesis of vitiligo was examined for clues to the pigmentary changes that may occur after treatment with topical imiquimod. The literature varies on the pigmentary changes induced by topical use of imiquimod. The US Food and Drugs Administration lists 68 reports of pigmentary changes out of a total of 1257 reports related to imiquimod lodged from 1997 to 2003. Some studies describe vitiligo-like hypopigmentation associated with imiquimod treatment of genital warts (as with the patient described in this report), molluscum contagiosum, basal cell carcinoma, extramammary Paget's disease and murine melanoma. Other studies report hyperpigmentation associated with imiquimod. The possible mechanisms of hypopigmentation associated with imiquimod treatment are discussed, including antibodies found in sera of patients with vitiligo to nonpigment cell antigens, cytoplasmic pigment cell antigens and pigment cell-surface antigens; stimulation by imiquimod of both the innate immune response and cell-mediated adaptive immunity; and increased sensitivity of melanocytes to oxidative stress. The vitiligo-like hypopigmentation following topical imiquimod treatment is in line with the mode of action of this drug.

  13. New-onset vitiligo during long-term, stable infliximab treatment of pityriasis rubra pilaris.

    Science.gov (United States)

    Mattox, Adam R; Chappell, Jeaneen A; Hurley, M Yadira

    2013-02-01

    Incidents of new-onset vitiligo attributed to infliximab therapy for rheumatoid arthritis and ulcerative colitis have been reported. Reported cases share a common theme in that symptoms manifested in close proximity to the initiation or significant dose increase of the medication. This case describes the presentation of infliximab-induced vitiligo in a patient using it for long-term treatment of stable pityriasis rubra pilaris. The patient was initiated and titrated to a stable dose of infliximab totaling 27 months' duration. He was able to achieve near-complete resolution of symptoms before developing depigmented patches consistent with vitiligo. Infliximab was discontinued. Tacrolimus 0.1% ointment and narrow-band ultraviolet B light successfully repigmented the patches. The association of discontinuing infliximab and resolution of vitiligo suggests infliximab had a role in this case. Though the mechanism of involvement is undetermined, infliximab may have induced an autoimmune process by paradoxically activating lymphocytes. Alternatively, infliximab antibodies may have led to the process by disrupting the normal balance of cytokines.

  14. Vitiligo after Diphencyprone for Alopecia Areata

    Science.gov (United States)

    Pires, Mario Cezar; Martins, João Mauricio; Montealegre, F.; Gatti, Flávia Romero

    2010-01-01

    The topical immunotherapy is used to treat alopecia areata and recalcitrant warts since the 1970s. Diphencyprone is a contact sensitizer used to treat dermatological conditions resulting from as altered immunological state, such as extensive alopecia areata, being partially effective and safe. Side effects include local eczema with blistering, regional lymphadenopathy and contact urticaria. Rare adverse effects include an erythema multiforme-like reaction, hyperpigmenttion, hypopigmentation, and vitiligo. We report a 30-year-old, Brazilian male who developed vitiligo lesions following DPCP therapy for alopecia areata. PMID:20585597

  15. Vitiligo after Diphencyprone for Alopecia Areata

    Directory of Open Access Journals (Sweden)

    Mario Cezar Pires

    2010-01-01

    Full Text Available The topical immunotherapy is used to treat alopecia areata and recalcitrant warts since the 1970s. Diphencyprone is a contact sensitizer used to treat dermatological conditions resulting from as altered immunological state, such as extensive alopecia areata, being partially effective and safe. Side effects include local eczema with blistering, regional lymphadenopathy and contact urticaria. Rare adverse effects include an erythema multiforme-like reaction, hyperpigmenttion, hypopigmentation, and vitiligo. We report a 30-year-old, Brazilian male who developed vitiligo lesions following DPCP therapy for alopecia areata.

  16. Clinical Observation of Traditional Chinese Medicine Prescription Freatiag Vitiligo%活血袪风法治疗白癜风的疗效观察

    Institute of Scientific and Technical Information of China (English)

    唐艳丽; 许爱娥

    2012-01-01

    目的:观察活血祛风法治疗进展期白癜风的临床效果.方法:根据中医辨证,证型为风血相搏的患者,口服以活血祛风法为主要治则的方剂治疗白癜风患者.结果:活血祛风法治疗白癜风具有较好的疗效,口服此方能有效控制进展期白癜风的发展.%Objective: The clinical efficacy of Traditional Chinese Medicine prescription which is used to treat 63 patients with vitiligo was observed. Medthods: According to treatment syndrome and differentiation,63 patients with vitiligo take the prescription,twice a day for 3 months. Results-. Traditional Chinese Medicine prescription has better efficacy on treating vitiligo and could control effectively the progress of vitiligo.

  17. Revised classification/nomenclature of vitiligo and related issues: the Vitiligo Global Issues Consensus Conference

    OpenAIRE

    2012-01-01

    During the 2011 International Pigment Cell Conference (IPCC), the Vitiligo European Taskforce (VETF) convened a consensus conference on issues of global importance for vitiligo clinical research. As suggested by an international panel of experts, the conference focused on four topics: classification and nomenclature; definition of stable disease; definition of Koebner’s phenomenon (KP); and ‘autoimmune vitiligo’. These topics were discussed in seven working groups representing different geogr...

  18. Association analysis revealed one susceptibility locus for vitiligo with immune-related diseases in the Chinese Han population.

    Science.gov (United States)

    Li, Shu; Yao, Weiyi; Pan, Qian; Tang, Xianfa; Zhao, Suli; Wang, Wenjun; Zhu, Zhengwei; Gao, Jinping; Sheng, Yujun; Zhou, Fusheng; Zheng, Xiaodong; Zuo, Xianbo; Sun, Liangdan; Zhang, Anping

    2015-07-01

    Generalized vitiligo is an autoimmune disease characterized by melanocyte loss, which results in patchy depigmentation of skin and hair, and is associated with an elevated risk of other immune-related diseases. However, there is no reported study on the associations between immune susceptibility polymorphisms and the risk of vitiligo with immune-related diseases. The aim of this study was to evaluate the potential influence of 10 single-nucleotide polymorphisms (SNPs) at 18q21.31 (rs10503019), 4p16.1 (rs11940117), 3q28 (rs1464510), 14q12 (rs2273844), 12q13.2 (rs2456973), 16q12.2 (rs3213758), 10q25.3 (rs4353229), 3q13.33 (rs59374417), and 10p15.1 (rs706779 and rs7090530) on vitiligo with immune-related diseases in the Chinese Han population. All SNPs were genotyped in 552 patients with vitiligo-associated immune-related diseases and 1656 controls using the Sequenom MassArray system. Data were analyzed with PLINK 1.07 software. The C allele of rs2456973 at 12q13.2 was observed to be significantly associated with vitiligo-associated immune-related diseases (autoimmune diseases and allergic diseases) (P = 0.0028, odds ratio (OR) = 1.27). In subphenotype analysis, the rs2456973 C allele was also significantly associated with early-onset vitiligo by comparing with controls (P = 0.0001) and in the case-only analysis (P = 0.0114). We confirmed that 12q13.2 was an important candidate locus for vitiligo with immune-related diseases (autoimmune diseases and allergic diseases) and affected disease phenotypes with early onset.

  19. Associations between TNF-α polymorphisms and susceptibility to rheumatoid arthritis and vitiligo: a meta-analysis.

    Science.gov (United States)

    Lee, Y H; Bae, S C

    2015-05-25

    We investigated whether the tumor necrosis factor-a (TNF-α) promoter -238 A/G and -308 A/G polymorphisms are associated with rheumatoid arthritis (RA) and vitiligo susceptibility. MEDLINE and EMBASE databases and a manual search were used to identify articles in which TNF-α polymorphisms were determined in RA or vitiligo patients and controls. Meta-analysis was used to examine the associations between the TNF-α -238 A/G polymorphism and RA and vitiligo using the allelic contrast and dominant models. Fifteen studies (10 RA and 5 vitiligo) involving 3678 cases and 4400 controls were considered. We observed an association between the TNF-α -238 A allele and RA when all subjects were considered [odds ratio (OR) = 0.686, 95% confidence interval (CI) = 0.476-0.968, P = 0.043]. After stratification by ethnicity, we found no association between the TNF-α -238 A allele and RA in European or Asian populations. We observed no association between the TNF-α -308 A allele and vitiligo (OR = 1.787, 95%CI = 0.894-3.573, P = 0.101). However, the adjusted OR by the trim-and-fill technique was significant (OR = 2.064, 95%CI = 1.138- 3.743). After stratification by geographic continent, the TNF-α -308 A allele was significantly associated with vitiligo in Middle Eastern populations (OR = 1.569, 95%CI = 1.224-2.013, P = 3.8 x 10(-5)). The TNF-α -238 A/G polymorphism was associated with RA susceptibility, and the TNF-α -308 A/G polymorphism may be a significant risk factor for vitiligo in Middle Eastern populations.

  20. Reduced immunohistochemical expression of adhesion molecules in vitiligo skin biopsies.

    Science.gov (United States)

    Reichert Faria, Adriane; Jung, Juliana Elizabeth; Silva de Castro, Caio César; de Noronha, Lucia

    2017-03-01

    Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin. The average value of the immunohistochemically positive reaction area of the vitiligo specimens was 3053.2μm(2), compared with the observed value of 3431.8μm(2) in non vitiligo skin (p=0.003) for laminin. The immuno-positive area was 7174.6μm(2) (vitiligo) and 8966.7μm(2) (non lesional skin) for integrin (p=0.042). A reduction in ICAM-1 and VCAM-1 expression in the basal layer of the epidermis in vitiligo samples was also observed (p=0.001 and pskin. Our results suggest that an impairment in adhesion exists in vitiligo skin, which is supported by the diminished immunohistochemical expression of laminin, beta1 integrin, ICAM-1 and VCAM-1.

  1. Clinical analysis of 339 cases of late onset vitiligo in Zhejiang province%浙江省339例迟发型白癜风临床分析

    Institute of Scientific and Technical Information of China (English)

    刘晓玉; 许爱娥; 金永红; 章莉; 孙学成; 黄博; 阮高波

    2009-01-01

    目的 探讨迟发型白癜风的临床特征和流行病学情况.方法 采用问卷调查形式对339例迟发型白癜风患者(发病年龄>30岁)进行临床研究,并以早发型患者(发病年龄0.05),while increased incidence of thyroid diseases and diabetes mellitus was noted in patients with late onset vitiligo(both P0.05).Compared with early onset vitiligo,late onset vitiligo was more vulnerable to environmental factors,and mental factors appeared to be the primary influencing factor.Conclusion There are some differences in clinical and epidemiological profiles between late onset vitiligo and early onset vitiligo.

  2. 他克莫司联合窄谱中波紫外线治疗白癜风的临床效果及其影响因素分析%Clinical effects and influence factors of tacrolimus combined with narrow band ultraviolet B in patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    侯占英; 刘芳; 黄珍; 刘万红

    2015-01-01

    目的:探讨他克莫司软膏联合窄谱中波紫外线治疗白癜风的临床效果及其影响因素。方法172例白癜风患者随机分为观察组与对照组各86例,观察组给予他克莫司软膏联合窄谱中波紫外线治疗,对照组仅给予窄谱中波紫外线照射治疗,对比两组患者的临床效果。根据治疗效果,分析性别、年龄、病程、病变类型、皮损位置等因素对观察组患者疗效的影响。结果观察组有效率为76.7%(66/86),显著高于对照组的52.3%(45/86)(P0.05)。结论他克莫司软膏联合窄谱中波紫外线照射能够快速、有效的恢复白癜风患者患处肤色、缩小皮损面积,但患者年龄、病程、皮损位置、病变类型等因素对疗效影响较大。%Objective To discuss the clinical effects and influence factors of tacrolimus combined with (NB-UVB) in patients with vitiligo. Methods A total of 172 patients with vitiligo were randomly divided into the ob-servation group (n=86) and the control group (n=86). The observation group were treated with tacrolimus combined with NB-UVB, while the control group were treated with NB-UVB only. Clinical effects were contrasted between the two groups. Then gender, age, course of disease, pathological type, vitiligo patches were analyzed according to the clinical effects to discuss the influence to the clinical effects. Results The effective rate of the observation group was 76.7%(66/86), significantly higher than 52.3%(45/86) in the control group (P0.05). Conclusion Tacrolimus ointment com-bined with NB-UVB can rapidly, effectively recover skin color and shrink lesion area in patient's with vitiligo, but pa-tient's age, course of disease, lesion location, pathological type and other factors would influence the curative effect to a large extent.

  3. Alopecia areata and vitiligo as primary presentations in a young male with human immunodeficiency virus

    Directory of Open Access Journals (Sweden)

    Li Xuan

    2014-01-01

    Full Text Available A 26-year-old Chinese male consulted with the team regarding his alopecia areata and vitiligo for which previous treatment was ineffective. The patient, a homosexual man, denied having a history of drug abuse and of blood transfusion. No member of his family had vitiligo or alopecia. Laboratory studies revealed that the serum for anti-human immunodeficiency virus (HIV antibody was positive. The patient′s CD4 lymphocyte count and CD4/CD8 ratio were both strikingly low (20 cells/mL and 0.04, but no other complaints or opportunistic infections were reported. One month after antiretroviral therapy, the patient′s alopecia areata dramatically improved, but no evident improvement in his vitiligo was found. This case is a very rare case of alopecia areata and vitiligo associated with HIV infection that might be attributed to the generation and maintenance of self-reactive CD8+ T-cells due to chronic immune activation with progressive immune exhaustion in HIV infection.

  4. Protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T ceils from vitiligo patients%茶多酚对CD8+T细胞杀伤白癜风患者黑素细胞的保护作用研究

    Institute of Scientific and Technical Information of China (English)

    欧阳杰; 吴纪龙; 周妙妮; 付丽芳; 许爱娥

    2013-01-01

    Objective To evaluate the protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T cells from vitiligo patients.Methods Skin tissue was resected from the margin of vitiligo lesions followed by the isolation and culture of CD8+ T lymphocytes,and from the normal skin of vitiligo patients followed by the isolation and culture of melanocytes.Flow cytometry was carried out to evaluate the purity of CD8+ T cells.The melanocytes were cocultured with the CD8 + T cells at different ratios followed by the evaluation of killing effect of CD8+ T cells.Various concentrations (200 and 400 μg/ml) of tea polyphenols were added into the co-culture system of CD8+ T cells and melanocytes at a ratio of 5 ∶ 1 followed by an additional culture of 48 hours.Then,flow cytometry was performed to detect the apoptosis in melanocytes in the coculture system.Results CD8+ T lymphocytes were successfully obtained from the marginal area of vitiligo lesions with a purity of more than 90%,which highly expressed the antigens CD137 and CD69.The coculture with CD8+ T cells markedly accelerated the apoptosis in melanocytes,while the accelerative effect was inhibited by tea polyphenols of 200 and 400 μg/ml.Conclusions The CD8+ T cells infiltrating the edge of vitiligo lesions display a potential destructive effect on autologous melanocytes from vitiligo patients,and tea polyphenols have a protective effect against the destruction of melanocytes by CD8+ T cells.%目的 探讨茶多酚对CD8+T细胞杀伤白癜风患者黑素细胞的保护作用.方法 取白癜风患者白斑边缘处皮片组织,培养CD8+T淋巴细胞,细胞纯度用流式细胞仪进行鉴定,通过和黑素细胞共培养验证其针对黑素细胞的杀伤作用.将不同浓度茶多酚加入CD8+T细胞和黑素细胞共培养的体系,用流式细胞仪检测黑素细胞的凋亡.结果 取白癜风皮损边缘表皮组织中成功培养CD8+T淋巴细胞,纯度达90%

  5. New approach in the treatment of refractory vitiligo: CO2 laser combined with betamethasone and salicylic acid solution.

    Science.gov (United States)

    Cunha, Paulo Rowilson; Scabine Pessotti, Nabila; Bonati Mattos, Camila; Salai, Ana Flavia

    2017-01-01

    The aim of this study is to evaluate the use of fractional carbon dioxide laser (CO2 ) with betamethasone and salicylic acid solution in the treatment of patients with refractory vitiligo in hands. Each hand of the patient was randomly assigned to one of two groups: lesion treated with fractional carbon dioxide laser associated with betamethasone and salicylic acid solution administration or lesion treated only with betamethasone and salicylic acid solution. We conclude that combined treatment with fractional carbon dioxide laser and betamethasone associated with salicylic acid solution could effectively and safely be used in the treatment of refractory vitiligo.

  6. Recent progress in the genetics of generalized vitiligo

    Institute of Scientific and Technical Information of China (English)

    Richard A. Spritz

    2011-01-01

    Vitiligo is an acquired disease characterized principally by patchy depigmentation of skin and overlying hair. Generalized vitiligo (GV), the predominant form of the disorder, results from autoimmune loss of melanocytes from affected regions. GV is a “complex trait”, inherited in a non-Mendelian polygenic, multifactorial manner. GV is epidemiologically associated with other autoimmune diseases, both in GV patients and in their close relatives, suggesting that shared genes underlie susceptibility to this group of diseases. Early candidate gene association studies yielded a few successes, such as PTPN22, but most such reports now appear to be false-positives. Subsequent genomewide linkage studies identified NLRPI and XBPI, apparent true GV susceptibility genes involved in immune regulation, and recent genome-wide association studies (GWAS) of GV in Caucasian and Chinese populations have yielded a large number of additional validated GV susceptibility genes.Together, these genes highlight biological systems and pathways that reach from the immune cells to the melanocyte, and provide insights into both disease pathogenesis and potential new targets for both treatment and even prevention of GV and other autoimmune diseases in genetically susceptible individuals.

  7. Vitiligo: a possible model of degenerative diseases.

    Directory of Open Access Journals (Sweden)

    Barbara Bellei

    Full Text Available Vitiligo is characterized by the progressive disappearance of pigment cells from skin and hair follicle. Several in vitro and in vivo studies show evidence of an altered redox status, suggesting that loss of cellular redox equilibrium might be the pathogenic mechanism in vitiligo. However, despite the numerous data supporting a pathogenic role of oxidative stress, there is still no consensus explanation underlying the oxidative stress-driven disappear of melanocytes from the epidermis. In this study, in vitro characterization of melanocytes cultures from non-lesional vitiligo skin revealed at the cellular level aberrant function of signal transduction pathways common with neurodegenerative diseases including modification of lipid metabolism, hyperactivation of mitogen-activated protein kinase (MAPK and cAMP response element-binding protein (CREB, constitutive p53-dependent stress signal transduction cascades, and enhanced sensibility to pro-apoptotic stimuli. Notably, these long-term effects of subcytotoxic oxidative stress are also biomarkers of pre-senescent cellular phenotype. Consistent with this, vitiligo cells showed a significant increase in p16 that did not correlate with the chronological age of the donor. Moreover, vitiligo melanocytes produced many biologically active proteins among the senescence-associated secretory phenotype (SAPS, such as interleukin-6 (IL-6, matrix metallo proteinase-3 (MMP3, cyclooxygenase-2 (Cox-2, insulin-like growth factor-binding protein-3 and 7 (IGFBP3, IGFBP7. Together, these data argue for a complicated pathophysiologic puzzle underlying melanocytes degeneration resembling, from the biological point of view, neurodegenerative diseases. Our results suggest new possible targets for intervention that in combination with current therapies could correct melanocytes intrinsic defects.

  8. Vitiligo: A Possible Model of Degenerative Diseases

    Science.gov (United States)

    Bellei, Barbara; Pitisci, Angela; Ottaviani, Monica; Ludovici, Matteo; Cota, Carlo; Luzi, Fabiola; Dell'Anna, Maria Lucia; Picardo, Mauro

    2013-01-01

    Vitiligo is characterized by the progressive disappearance of pigment cells from skin and hair follicle. Several in vitro and in vivo studies show evidence of an altered redox status, suggesting that loss of cellular redox equilibrium might be the pathogenic mechanism in vitiligo. However, despite the numerous data supporting a pathogenic role of oxidative stress, there is still no consensus explanation underlying the oxidative stress-driven disappear of melanocytes from the epidermis. In this study, in vitro characterization of melanocytes cultures from non-lesional vitiligo skin revealed at the cellular level aberrant function of signal transduction pathways common with neurodegenerative diseases including modification of lipid metabolism, hyperactivation of mitogen-activated protein kinase (MAPK) and cAMP response element-binding protein (CREB), constitutive p53-dependent stress signal transduction cascades, and enhanced sensibility to pro-apoptotic stimuli. Notably, these long-term effects of subcytotoxic oxidative stress are also biomarkers of pre-senescent cellular phenotype. Consistent with this, vitiligo cells showed a significant increase in p16 that did not correlate with the chronological age of the donor. Moreover, vitiligo melanocytes produced many biologically active proteins among the senescence-associated secretory phenotype (SAPS), such as interleukin-6 (IL-6), matrix metallo proteinase-3 (MMP3), cyclooxygenase-2 (Cox-2), insulin-like growth factor-binding protein-3 and 7 (IGFBP3, IGFBP7). Together, these data argue for a complicated pathophysiologic puzzle underlying melanocytes degeneration resembling, from the biological point of view, neurodegenerative diseases. Our results suggest new possible targets for intervention that in combination with current therapies could correct melanocytes intrinsic defects. PMID:23555779

  9. Vitiligo: a possible model of degenerative diseases.

    Science.gov (United States)

    Bellei, Barbara; Pitisci, Angela; Ottaviani, Monica; Ludovici, Matteo; Cota, Carlo; Luzi, Fabiola; Dell'Anna, Maria Lucia; Picardo, Mauro

    2013-01-01

    Vitiligo is characterized by the progressive disappearance of pigment cells from skin and hair follicle. Several in vitro and in vivo studies show evidence of an altered redox status, suggesting that loss of cellular redox equilibrium might be the pathogenic mechanism in vitiligo. However, despite the numerous data supporting a pathogenic role of oxidative stress, there is still no consensus explanation underlying the oxidative stress-driven disappear of melanocytes from the epidermis. In this study, in vitro characterization of melanocytes cultures from non-lesional vitiligo skin revealed at the cellular level aberrant function of signal transduction pathways common with neurodegenerative diseases including modification of lipid metabolism, hyperactivation of mitogen-activated protein kinase (MAPK) and cAMP response element-binding protein (CREB), constitutive p53-dependent stress signal transduction cascades, and enhanced sensibility to pro-apoptotic stimuli. Notably, these long-term effects of subcytotoxic oxidative stress are also biomarkers of pre-senescent cellular phenotype. Consistent with this, vitiligo cells showed a significant increase in p16 that did not correlate with the chronological age of the donor. Moreover, vitiligo melanocytes produced many biologically active proteins among the senescence-associated secretory phenotype (SAPS), such as interleukin-6 (IL-6), matrix metallo proteinase-3 (MMP3), cyclooxygenase-2 (Cox-2), insulin-like growth factor-binding protein-3 and 7 (IGFBP3, IGFBP7). Together, these data argue for a complicated pathophysiologic puzzle underlying melanocytes degeneration resembling, from the biological point of view, neurodegenerative diseases. Our results suggest new possible targets for intervention that in combination with current therapies could correct melanocytes intrinsic defects.

  10. Vitiligo: A review of some facts lesser known about depigmentation

    Directory of Open Access Journals (Sweden)

    James J Nordlund

    2011-01-01

    Full Text Available Vitiligo is a disorder that causes the destruction of melanocytes. It has three important factors underlying this destruction. The depigmented skin has many aberrant functions such as a muted response to contact allergens, a phenomenon also seen in mice that depigment. The white skin of those with vitiligo does not form non-melanoma skin cancers although the white skin of albinos, which has a similar color as vitiligo, is highly susceptible to skin cancer.

  11. Interferon-gamma Inhibits Melanogenesis and Induces Apoptosis in Melanocytes: A Pivotal Role of CD8+ Cytotoxic T Lymphocytes in Vitiligo.

    Science.gov (United States)

    Yang, Lili; Wei, Yi; Sun, Yue; Shi, Weimin; Yang, Ji; Zhu, Lubing; Li, Ming

    2015-07-01

    Increased expression of the cytokine interferon (IFN)-γ plays a pivotal role in vitiligo-induced depigmentation. However, the major source of IFN-γ in vitiligo patients and the mechanisms underlying melanocyte destruction are unknown. In this study, a large number of skin infiltrating IFN-γ+ cells and CD8+ T cells were detected in progressive vitiligo. Among the peripheral blood mononuclear cells (PBMCs) of vitiligo patients, CD8+ cytotoxic T lymphocytes (CTLs) that express IFN-γ exhibited significant expansion, which suggests that activated CTLs are the main source of increased IFN-γ in progressive vitiligo. An in vitro analysis demonstrated that IFN-γ inhibits melanogenesis in primary cultured human melanocytes by altering melanogenic enzyme mRNA expression and, more importantly, that IFN-γ directly induces melanocyte apoptosis. Our data indicate that vitiligo pathophysiology may be linked to globally activated CD8+ CTL subpopulations, which produce increased IFN-γ and induce melanocyte dysfunction and apoptosis.

  12. Clinical analysis of Liquid Nitrogen Cryotherapy combined with Vitiligo Tincture in treatment of focal vitiligo%液氮冷冻联合白癜酊治疗局限性白癜风临床分析

    Institute of Scientific and Technical Information of China (English)

    杨龙波; 林兰

    2011-01-01

    Objective :To investigate the clinical efficacy of liquid nitrogen cryotherapy and vitiligo tincture in treatment of limit vitiligo.Methods :140 cases of limit vitiligo patients were in our hospital from October 2008 to September 2010.80 patients were given liquid nitrogen cryotherapy and vitiligo tincture treatment,60 patients were given liquid nitrogen cryotherapy treatment only,observe treatment effect.Results :To evaluate treatment effect after 2 months treatment,efficient rate of combined treatment group was 93.7%, cryotherapy group was 76.7%, the difference of two groups was statistically significant(x2=14.38,P<0.05).Conclusion:The efficient rate of liquid nitrogen cryotherapy combined with vitiligo tincture in treatment of vitiligo is good, and it is worthy of clinical application.%目的:探讨液氮冷冻联合白癜酊治疗局限性白癜风的临床疗效.方法:选择2008年10月~2010年9月本院收治的局限性白癜风患者140例,其中80例给予液氮冷冻联合白癜酊治疗,60例给予液氮冷冻治疗,观察两组治疗效果.结果:治疗2个月后评价治疗效果,联合治疗组有效率为93.7%,冷冻治疗组有效率为76.7%,两组比较差异有统计学意义(χ2=14.38,P<0.05).结论:液氮冷冻联合药物外涂治疗白癜风有效率较高,值得临床应用.

  13. Meta-analysis of the TNF-α-308G/A polymorphism and vitiligo risk.

    Science.gov (United States)

    Nie, G; Qi, J H; Huang, C W; Yang, T; Shi, N; Chen, Y J

    2015-12-17

    Several case-control studies have been conducted to investigate the association between the tumor necrosis factor-α (TNF-α)-308G/A polymorphism and vitiligo risk. However, the results of these studies are inconsistent; therefore, we attempted to comprehensively evaluate the association between TNF-α-308G/A polymorphism and vitiligo risk via a meta-analysis. Studies reporting the association between TNF-α-308G/A polymorphism and vitiligo risk were retrieved from PubMed and EmBase databases. Data were extracted from these studies and the pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association. Six case-control studies including 1391 vitiligo cases and 2455 control subjects were included in this meta-analysis. The overall results showed the lack of a significant difference in TNF-α-308G/A genotype distribution between the patients and controls when the G allele and GG, GG + GA, GG, and GG genotypes were compared against the A allele and the GA + AA, AA, AA, and GA genotypes, respectively (ORs = 0.65, 0.53, 0.63, 0.41, 0.55; 95%CI = 0.35-1.23, 0.24-1.18, 0.10-4.09, 0.08-1.97, 0.25-1.21; P = 0.188, 0.121, 0.627, 0.264, 0.135, respectively). This meta-analysis suggests that the TNF-α-308G/A polymorphism may not be associated with vitiligo risk. As few studies are available in this field and current evidence remains limited, these results must be corroborated with well-designed and larger studies in the future.

  14. Comparative Study of Efficacy of Epidermal Melanocyte Transfer Versus Hair Follicular Melanocyte Transfer in Stable Vitiligo

    Science.gov (United States)

    Donaparthi, Navya; Chopra, Ajay

    2016-01-01

    Background: Vitiligo surgery has come up a long way from punch skin grafts to epidermal cell suspension and latest to the extracted hair follicle outer root sheath cell suspension (EHFORSCS) transplantation. The progressive development from one technique to the other is always on a quest for the best. In the latest development, EHFORSCS, which is an enriched source of follicular inactive melanocyte (melanocyte stem cells), seems to be a good addition to the prevailing cell-based therapies for vitiligo. However, it needs to be explored further in larger, clinical trials. Methodology: A total of 11 patients with sixty stable vitiligo sites attending dermatology outpatient department were included for the open-labeled, prospective, comparative study. The sites were sequentially distributed into two groups of thirty each. Sites of one group were subjected to epidermal melanocyte transfer (EMT) and the others to hair follicular melanocyte transfer (HFMT). Response to treatment was evaluated on the basis of degree of repigmentation; final evaluation of area of involvement was done after completion of 6 months. Results: At the end of 6 months, repigmentation >90% was observed in 83.33% patches of EMT group and 43.33% in HFMT group. Repigmentation >75% was observed in 90% of patches in Group A and 43.34% of patches in Group B, respectively. There was statistically significant difference in the overall pigmentation between these two groups. Conclusion: Both noncultured autologous epidermal cell suspension transfer and noncultured EHFORSCS transfer are safe and effective surgical modalities in the management of stable vitiligo though EMT has shown a better response in the present study. Outer root sheath cell suspension transfer is a novel, minimally invasive technique in its nascent stage in the surgical management of vitiligo which requires further larger clinical trials for evaluation of its efficacy. PMID:27904182

  15. 白癜风表皮黑素细胞超微结构及小眼畸形相关转录因子转录调控研究%Ultrastructure and transcriptional activity of MITF in epidermal melanocytes from patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    王平; 洪为松; 章莉; 洪健; 郑俊惠; 许爱娥

    2010-01-01

    Objective To analyze the relationship of melanocyte ultrastructure and expression of microphthalmia-associated transcription factor (MITF) as well as tyrosinase-related proteins (TRP) transcriptionally modulated by MITF to clinical types and duration of vitiligo.Methods Epidermal sheets were taken by suction blisters respectively from lesional,perilesional,and normal skin of 12 patients with vitiligo vulgaris (VV) and 8 with segmental vitiligo (SV).The duration of vitiligo varied from 3 to 300 months in these patients. Transmission electron microscopy was performed in 10 patients with vitiligo,including 6 cases of VV and 4 cases of SV.Epidermal melanocytes from normal skin of 20 patients were subjected to culture followed by Western blot to detect the expression level of MITF and some molecules transcriptionally modulated by MITF,including tyrosinase (TYR),TYR-related protein-1(TYRP1),and TYR-related protein-2 (TYRP2) in cultured melanocytes.Results Epidermal melanocytes were absent in lesional skin of 7 out of 10 patients observed for ultrastructural alterations,whereas melanocytes with reduced or absent melanin (melanosome) could accidently be seen in lesional skin of 1 patient with short-standing vitiligo and 2 patients with long-standing vitiligo.In perilesional skin.abnormal ultrastructure of melanocytes was found in 3 with a duration of vitiligo less than 15 months among 6 patients with VV,and in 1 out of 4 patients with SV.The down-regulated expression of MITF was consistent with that of TYR,TYRP1 and TYRP2 in cultured epidermal melanocytes from normal skin of patients with VV;in those from patients with SV,the down-regulated expression was observed only in MITF,while the expressions of TYR,TYRP1 and TYRP2 were nearly normal.Conclusion Differences may exist between VV and SV in the ultrastructure as well as mechanisms of transcriptional modulation by MITF in epidermal melanocytes.%目的 研究白癜风黑素细胞超微结构和小眼畸形相关转录因

  16. Catalase (CAT) promoter and 5'-UTR genetic variants lead to its altered expression and activity in vitiligo.

    Science.gov (United States)

    Mansuri, M S; Jadeja, S D; Singh, M; Laddha, N C; Dwivedi, M; Begum, R

    2017-05-25

    Oxidative stress is considered to be the initial event during the course of vitiligo. Catalase is mainly involved in the defense against oxidizing agents in the cell through detoxifying H2 O2 . Aims of the present study were 1) to assess the erythrocyte catalase activity and LPO levels, as well as CAT mRNA expression in skin and blood; 2) to investigate CAT promoter rs7943316, rs1001179; 5'-UTR rs1049982 and exon (rs17886350, rs11032709, rs17880442, rs35677492) polymorphisms and; 3) to perform genotype-phenotype/ haplotype correlation analyses, in vitiligo patients and controls from Gujarat. Catalase activity and lipid peroxidation levels were measured spectrophotometrically. CAT mRNA levels were estimated using Real-time PCR by SYBR-Green method. SNP genotyping was performed using PCR-RFLP/ ARMS-PCR methods. Vitiligo patients showed significantly decreased CAT mRNA-expression in lesional, non-lesional skin and in blood with reduced catalase activity compared to that of controls. CAT -89A/T and -20T/C polymorphisms were significantly associated with patients, especially with active and generalized vitiligo whereas, no association was observed for -262G/A and exon polymorphisms. 'A-262 T-89 C-20 ' haplotype with variant alleles was found to be associated with 6.4-fold risk of vitiligo. Genotype/ haplotype-phenotype correlation analyses revealed that individuals with susceptible genotypes/ haplotype for CAT -89 A/T and -20 T/C polymorphisms showed significantly decreased catalase mRNA/activity, and only -89 A/T polymorphisms showed significantly increased lipid peroxidation levels, as compared to wild type genotypes/ haplotype. In conclusion, the present study proposes the crucial role of catalase and its allelic variants in oxidative stress mediated pathogenesis of vitiligo. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  17. EFFICACY OF LEECH THERAPY AND AYURVEDIC COMPOUND IN VITILIGO: A QUASI EXPERIMENTAL STUDY

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    Panigrahi Hemanta kumar

    2012-12-01

    Full Text Available This interventional, quasi-experimental study (pre-post test design was conducted at Ayurveda Central Research Institute, New Delhi to study the efficacy of Leech Therapy along with some Ayurvedic medicine in vitiligo. Patients were selected by non-probability purposive sampling method after obtaining an informed consent. Twenty patients suffering from vitiligo (12 male and 08 female, aged more than 15 years and involving less than 25% of body surface area were enrolled. All the patients were kept in a single group. All the patients were called to OPD for Leech application once in a week. The patients were followed up weekly for first month and later on fortnightly for five months to monitor the improvement and adverse effects. The mean re-pigmentation achieved was 60 % (VASI score of 3.69, while the arrest of progression (stationary was noted in 35 % of patients. In 5% of patient it was spreading. We recommend that leech therapy along with internal administration of Ayurvedic medicine is effective in the treatment of Vitiligo.

  18. Positive pleiotropic effects of HMG-CoA reductase inhibitor on vitiligo

    OpenAIRE

    2004-01-01

    Abstract Background HMG-CoA reductase inhibitors (statins) are commonly used in medicine to control blood lipid disorder. Large clinical trials have demonstrated that statins greatly reduces cardiovascular-related morbidity and mortality in patients with and without coronary artery disease. Also, the use of HMG-CoA reductase inhibitors has been reported to have immunosuppressive effects. Case presentation We describe an unusual case of regression of vitiligo in a patient treated with high dos...

  19. The Vitiligo Working Group recommendations for narrowband ultraviolet B light phototherapy treatment of vitiligo.

    Science.gov (United States)

    Mohammad, Tasneem F; Al-Jamal, Mohammed; Hamzavi, Iltefat H; Harris, John E; Leone, Giovanni; Cabrera, Raúl; Lim, Henry W; Pandya, Amit G; Esmat, Samia M

    2017-05-01

    Treatment of vitiligo with narrowband ultraviolet B light (NBUVB) is an important component of the current standard of care. However, there are no consistent guidelines regarding the dosing and administration of NBUVB in vitiligo, reflected by varied treatment practices around the world. To create phototherapy recommendations to facilitate clinical management and identify areas requiring future research. The Vitiligo Working Group (VWG) Phototherapy Committee addressed 19 questions regarding the administration of phototherapy over 3 conference calls. Members of the Photomedicine Society and a group of phototherapy experts were surveyed regarding their phototherapy practices. Based on comparison and analysis of survey results, expert opinion, and discussion held during conference calls, expert recommendations for the administration of NBUVB phototherapy in vitiligo were created. There were several areas that required further research before final recommendations could be made. In addition, no standardized methodology was used during literature review and to assess the strength of evidence during the development of these recommendations. This set of expert recommendations by the VWG is based on the prescribing practices of phototherapy experts from around the world to create a unified, broadly applicable set of recommendations on the use of NBUVB in vitiligo. Copyright © 2017 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.

  20. Myths and facts about vitiligo: An epidemiological study

    Directory of Open Access Journals (Sweden)

    Suja Abraham

    2015-01-01

    Full Text Available Vitiligo is a pigmentary disorder associated with many disease conditions that necessitates multiple drug regimens, which make the treatment complicated. This stigmatic disease forces the patient to approach all system of medicines as well as alternative medicines of non proven value, which further worsens the situation. At the same time the nonadherence to the treatment reflects poor prognosis, which is misunderstood for lack of response resulting in poor faith to the medications. The aim of this work was to assess the patient compliance and the factors affecting, and to monitor the adverse effects as well as drug interactions. The study was carried out in the Institute of Applied Dermatology for a period of one year. Patient compliance was assessed using Morisky Medication Adherence Scale and found that 71% of the patients were low adherent to medications. Family support, faith in doctor, higher educational status and effectiveness of the treatment were some of the reasons for medication adherence whereas forgetfulness, feasibility, occupational problem, polypharmacy, longer duration of treatment and the feeling that the disease under is control were some of the reasons listed for nonadherence. Three adverse reactions were reported with narrow band ultraviolet B and topical tacrolimus therapy and they were categorized into possible and probable according to causality assessment by Naranjo scale. Five drug interactions were reported and the causality assessment was done using drug interaction probability scale. None of the reactions were serious or life threatening. The present study revealed the hurdles in providing safe and effective treatment to the patients and also it suggest the need of doing more research on this disease since there is a general belief that vitiligo is an incurable disease.

  1. Study of palmar dermatoglyphics in vitiligo and normal individuals

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    Azra Mubeen Karnul

    2015-04-01

    Full Text Available Background & Objective: Vitiligo is a common idiopathic & hereditary disease characterized by one or more patches of de-pigmented skin due to degeneration or disappearance of cutaneous melanocytes. Variations in dermatoglyphic pattern are noted in many genetic & hereditary disorders. Altered dermatoglyphics pattern is well established as a diagnostic aid in such disorders. Early diagnosis is also possible. Therefore this Study was conducted to analyse dermatoglyphic patterns in Vitiligo cases & their deviations from normal individuals & to find out any existence of specific dermatoglyphic pattern in Vitiligo cases. Method: Study conducted across 100 Vitiligo cases and 100 normal individuals. Finger tips and palmar prints were studied. Result: Finger tips pattern of Vitiligo males showed increased loop patterns on 2nd, 3rd & 4th finger tips of both hands. There is an overall highly significant diminution in whorl pattern in hands of males. Arch pattern is shown in all finger tips of female cases exceedingly .The main line formula evidenced in Vitiligo cases is 7 6 5’ & while as reference sample 11 9 7 in both sexes. Distal displacement of axial triradius is increased significantly in left hands of female cases. ATD angle is reduced significantly in male vitiligo cases. Classical distribution formula for palmar pattern frequency is altered as the pattern frequency is increased in hypothenar & ID2 of right hands of vitiligo males while as hypothenar & ID1 in right hands of vitiligo females. Conclusion: Deviations in dermatoglyphic pattern might be a marker for individual diagnosis of vitiligo & a simple & inexpensive means to determine the disease which have hereditary background & can be employed as a method for screening Vitiligo.

  2. 抗核抗体、免疫球蛋白及补体在儿童白癜风疾病中的价值探讨%Analysis on values of ANAs,immunoglobulins and complements in children patients with vitiligo

    Institute of Scientific and Technical Information of China (English)

    蒋跃根; 曾学思; 程险峰; 王维; 王峰来; 蔡秀玲

    2016-01-01

    目的:探讨白癜风患儿抗核抗体、免疫球蛋白(IgG 、IgM 和 IgA)和补体(C3和 C4)在本病中的临床价值。方法以调查表形式收集145例白癜风患儿的临床资料,应用统计学软件对数据进行统计学分析。采用间接免疫荧光法和免疫印迹法检测患儿血清抗核抗体及12种自身抗体谱,免疫透射比浊法检测 IgG 、IgM 、IgA 和C3、C4。结果调查发现,145例患儿的病程为1个月至5年,白斑的初发部位以头面部最为多见,以轻度和散发型白斑类型居多;大部分患儿病情处于进展期,30.35%患儿有白癜风或者自身免疫性疾病的家族史。对其血清抗核抗体检测结果发现,共有13例为阳性,其中8例存在特定自身抗体阳性。白癜风组 IgG 、IgA 水平明显低于对照组,差异有统计学意义(P<0.01),而 IgM 水平差异无统计学意义(P>0.05)。白癜风组患儿 C4血清水平明显低于对照组,差异有统计学意义(P=0.035),但 C3水平差异无统计学意义(P>0.05)。结论白癜风患儿的抗核抗体水平与健康儿童几乎无异,但存在较为明显的体液免疫功能异常。%Objective To study the clinical value of antinuclear antibodies(ANA) ,immunoglobulins(IgG ,IgM and IgA) and complements(C3 and C4) in the children patients with vitiligo .Methods The clinical data of 145 pedi-atric patients with vitiligo were collected by using the uniform questionnaire and the data were processed by using the statistical software .The indirect immunofluorescence and immunoblotting method were adopted to detect serum AN-As and 12 kinds of autoantibodies .IgG ,IgM ,IgG and C3 ,C4 were detected by using the immunological transmission turbidimetry method .Results The investigation found that the disease course in 145 cases of vitiligo was from 1 month to 5 years ,the primary sites of vitiligo were mainly the head ,which were dominated by mild and

  3. Fabrication of anti-vitiligo ointment containing Psoralea corylifolia: in vitro and in vivo characterization

    Science.gov (United States)

    Hussain, Irshad; Hussain, Nisar; Manan, Abdul; Rashid, Abdur; Khan, Barkat; Bakhsh, Sattar

    2016-01-01

    Background Vitiligo is a repugnant and odious dermatological malady of the time. It has an detrimental impact on the pigmentation of the human skin as a result of the destruction of cutaneous melanocytes. It affects 1%–2% of the population worldwide. Different therapeutic regimens have been deployed to treat vitiligo, but none of them could stand alone to be stated as a perfect cure. Recently, a change has been observed through novel experimental-designed optimization leading to the development of an anti-vitiligo ointment containing Psoralea corylifolia (PC) seed powder. Aim The aim of this study was to explore the clinical outcomes of ointment containing powdered seeds of PC. Materials and methods Guided by the protocol Response Surface Methodology, 13 formulations of concentration variance of permeation enhancers were prepared. The formulation fulfilling the required criteria (pH; temperature stability tests at 8°C±0.1°C, 25°C±0.1°C and 40°C±0.1°C; and the physical properties such as color, bleeding and rheology) was selected for clinical trials. Fourier transform infrared spectroscopy studies of seed powder of PC and selected formulation of the seed powder were performed. After obtaining informed consents and with prior approval of university and hospital ethical review boards, 20 patients (age range 25–65 years) were included in the present study. Formulations were applied on the affected body parts of patients, and some affected portion of the same patient was taken as control (self-control study design). The pigmentation of white spots of vitiligo was photographically evaluated before, during and after 12 weeks of treatment. Analysis of the measured values was performed using GraphPad Prism version 5 statistical software. A paired sample t-test was performed to observe variation between repigmented patches and white patches of self-control. Results Hydrophilic ointment (10% w/w) prepared with seed powder of PC was fabricated. The ointment was

  4. Efficacy of topical calcineurin inhibitors in vitiligo.

    Science.gov (United States)

    Wong, Russell; Lin, Andrew N

    2013-04-01

    Topical tacrolimus and pimecrolimus are indicated for the treatment of atopic dermatitis, but they have been studied in many off-label uses. We reviewed the English language literature to define their roles in treatment of vitiligo. Double-blind studies show that tacrolimus 0.1% ointment combined with excimer laser is superior to placebo, especially for UV resistant areas, such as bony prominences of the extremities. When used alone, tacrolimus 0.1% ointment is almost as effective as clobetasol propionate 0.05% ointment. Other studies suggest it can also be effective for facial lesions. Double blind studies show that pimecrolimus 1% cream combined with narrow band UVB is superior to placebo, especially for facial lesions. Additional studies would further clarify the role of topical calcineurin inhibitors in vitiligo. © 2013 The International Society of Dermatology.

  5. Is lesional stability in vitiligo more important than disease stability for performing surgical interventions? results from a multicentric study

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    Imran Majid

    2016-01-01

    Full Text Available Background: Ensuring stability of the disease process is essential for undertaking surgical intervention in vitiligo. However, there is no consensus regarding the minimum duration of stability or the relative importance of disease and lesional stability in selecting patients for vitiligo grafting. Aim: This multicentric study aims to assess the relative importance of lesional and disease stability on selecting patients for vitiligo grafting. Materials and Methods: One hundred seventy patients were recruited into the study and divided into two groups: Group A with lesional stability of >1 year but overall disease stability of only 6-11 months and Group B with overall disease stability of >1 year. Patients underwent either tissue or cellular vitiligo grafting on the selected lesions and the repigmentation achieved was scored from 0 (no repigmentation to 6 (100% repigmentation. Repigmentation achieved on different sites of the body was compared between the two groups. Adverse effects at both the donor and the recipient sites were also compared. Results: Of the 170 patients who were enrolled, 82 patients were placed in Group A and 88 patients in Group B. Average repigmentation achieved (on scale of 0 to 6 was 3.8 and 4.04 in Group A and Group B, respectively. In Group A, ≥90% repigmentation was achieved in 36.6% (30/82 patients, while 37.5% (33/88 achieved similar results in Group B. Additionally, 47.6% (39/82 and 53.4% (47/88 of cases achieved partial repigmentation in Group A and Group B, respectively. Perigraft halo was the commonest adverse effect observed in both groups. Statistical analysis revealed no significant differences between the two groups with respect to the repigmentation achieved or adverse effects observed. Repigmentation achieved was the best on the face and neck area, while acral areas responded the least. Conclusions: Lesional stability seems to be as relevant as the overall disease stability in selecting patients for

  6. Oxidative stress-induced overexpression of miR-25: the mechanism underlying the degeneration of melanocytes in vitiligo.

    Science.gov (United States)

    Shi, Q; Zhang, W; Guo, S; Jian, Z; Li, S; Li, K; Ge, R; Dai, W; Wang, G; Gao, T; Li, C

    2016-03-01

    Oxidative stress has a critical role in the pathogenesis of vitiligo. However, the specific molecular mechanism involved in oxidative stress-induced melanocyte death is not well characterized. Given the powerful role of microRNAs (miRNAs) in the regulation of cell survival as well as the fact that the generation of miRNAs can be affected by oxidative stress, we hypothesized that miRNAs may participate in vitiligo pathogenesis by modulating the expression of vital genes in melanocytes. In the present study, we initially found that miR-25 was increased in both serum and lesion samples from vitiligo patients, and its serum level was correlated with the activity of vitiligo. Moreover, restoration of miR-25 promoted the H2O2-induced melanocyte destruction and led to the dysfunction of melanocytes. Further experiments proved that MITF, a master regulator in melanocyte survival and function, accounted for the miR-25-caused damaging impact on melanocytes. Notably, other than the direct role on melanocytes, we observed that miR-25 inhibited the production and secretion of SCF and bFGF from keratinocytes, thus impairing their paracrine protective effect on the survival of melanocytes under oxidative stress. At last, we verified that oxidative stress could induce the overexpression of miR-25 in both melanocytes and keratinocytes possibly by demethylating the promoter region of miR-25. Taken together, our study demonstrates that oxidative stress-induced overexpression of miR-25 in vitiligo has a crucial role in promoting the degeneration of melanocytes by not only suppressing MITF in melanocytes but also impairing the paracrine protective effect of keratinocytes. Therefore, it is worthy to investigate the possibility of miR-25 as a potential drug target for anti-oxidative therapy in vitiligo.

  7. Melanoma versus vitiligo: Clinical aspects and implications for immunotherapy

    NARCIS (Netherlands)

    Teulings, H.E.

    2015-01-01

    Het onderzoek beschreven in dit proefschrift richt zich op de klinische en immunologische aspecten van twee huidziekten, melanoom en vitiligo, waarbij pigment een grote rol speelt. Vitiligo is een auto-immuunziekte, gericht tegen de melanocyten (pigmentcellen) in de huid, die zich kenmerkt door het

  8. Lived Experience of Women Suffering from Vitiligo: A Phenomenological Study

    Science.gov (United States)

    Borimnejad, Leili; Yekta, Zohreh Parsa; Nasrabadi, Alireza Nikbakht

    2006-01-01

    Vitiligo is a chronic skin disease, which through change of appearance and body image, exerts a devastating effect on people, especially women. The objective of this study is to explore lived experience of women with Vitiligo by the hermeneutic phenomenology method. The purposive sample consisted of 16 Iranian women. Data analysis followed…

  9. Angiotensin converting enzyme (ACE) gene polymorphism in vitiligo: protective and predisposing effects of genotypes in disease susceptibility and progression.

    Science.gov (United States)

    Tippisetty, Surekha; Ishaq, Mohammed; Komaravalli, Prasanna Latha; Jahan, Parveen

    2011-01-01

    Vitiligo is a depigmenting skin disorder with profound heterogenity in its aetio-pathophysiology, and is associated with inter-individual variation in progression of disease. Angiotensin converting enzyme (ACE) is a regulator of renin angiotensin system (RAS) that plays an important role in the physiology of the vasculature, blood pressure, inflammation, adipocyte distribution of various diseases. The present study was carried out in 243 vitiligo patients (132 males and 111 females), aged between 3-62 years with a mean age at onset of 21.6  ±  13.6 yrs, and in 205 healthy controls of south Indian origin. The main objectives of the present study were to evaluate the ACE I/D (insertion/deletion) polymorphism in the patient and control groups. Further, I/D genotypes were compared among the patients with and without the family history of vitiligo as well as the progression of the disease, through polymerase chain reaction (PCR) methods.The results revealed a highly significant association of DD genotype with disease susceptibility (p vitiligo (p < 0.05) in terms of early age at onset. Further, the pre-dominance of ID genotype among patients revealed its association with a slow progression of the disease (p < 0.05). The present study is the first report to highlight the protective role of II genotype and the significant association of ID genotype with slow progression of the disease.

  10. Vitiligo nonsurgical treatment: a review of latest treatment researches.

    Science.gov (United States)

    Zhang, Yanfei; Mooneyan-Ramchurn, Jessini Soranambal; Zuo, Nan; Feng, Yiguo; Xiao, Shengxiang

    2014-01-01

    Vitiligo is a common depigmenting acquired disorder affecting about 1-2% of the world population, regardless of race, ethnic background, or gender. It is characterized by the appearance of milky white maculae because of a loss of melanocytes. The disfiguring nature of vitiligo causes high psychosocial morbidity. This is especially pronounced in populations with darker skin tone, likely because of the marked contrast. A variety of nonsurgical treatment regimens are currently employed in vitiligo. We reviewed the latest studies carried out on different nonsurgical treatment modalities used in vitiligo. All nonsurgical treatment aid to repigment or depigmentation the skin, however, many of them require a prolonged treatment course and may yield minimal results as well as carry unwanted side effects. There is a need for further research into the causes of vitiligo and into discovering better treatments.

  11. GRACE评分对NSTEACS合并CKD患者住院期间全因病死率的预测价值%Predictive Value of the Global Registry of Acute Coronary Event Score for In-hospital Mortality of Patients with Non-segment Elevated Acute Coronary Syndrome and Chronic Kidney Diseases

    Institute of Scientific and Technical Information of China (English)

    李红; 金泽宁; 孟帅; 唐熠达

    2016-01-01

    目的 探讨全球急性冠状动脉事件注册(global registry of acute coronary events,GRACE)评分对非ST段抬高型急性冠状动脉综合征(non-segment elevated acute coronary syndromes,NSTEACS)合并慢性肾脏疾病(chronic kidney disease,CKD)患者住院期间全因病死率的预测价值.方法 选取2012年1月-2014年12月在我院住院期间发生全因死亡的NSTEACS合并CKD 60例作为病死组,随机抽取同期未发生死亡的NSTEACS合并CKD 60例作为生存组,两组均进行GRACE评分.比较两组一般资料、GRACE评分危险分层构成比及中位数(四分位数).采用受试者工作特征(ROC)曲线分析GRACE评分对NSTEACS合并CKD患者住院期间全因病死率的预测价值.结果 两组一般资料中Killip分级、心电图ST段改变、低密度脂蛋白胆固醇及空腹血糖比较差异有统计学意义(P<0.05);两组GRACE评分危险分层构成比比较差异有统计学意义(P<0.05),病死组GRACE评分中位数(四分位数)明显高于生存组,差异亦具有统计学意义(P<0.05).ROC曲线显示GRACE评分对NSTEACS合并CKD患者住院期间全因病死率预测价值良好[AUC 0.864,95%可信区间(0.763,0.911)].结论 GRACE评分对NSTEACS合并CKD患者住院期间全因病死率具有良好的预测价值.

  12. Current and emerging therapy for the management of vitiligo

    Directory of Open Access Journals (Sweden)

    Alicia Cecile Borderé

    2009-03-01

    Full Text Available Alicia Cecile Borderé, Jo Lambert, Nanny van GeelUniversity Hospital of Ghent, Department of Dermatology, Ghent, BelgiumAbstract: Vitiligo is an acquired cutaneous disorder of pigmentation, with an incidence of 0.5% to 2% worldwide. There are three major hypotheses for the pathogenesis of vitiligo that are not exclusive of each other: biochemical/cytotoxic, neural and autoimmune. Recent data provide strong evidence supporting an autoimmune pathogenesis of vitiligo. As vitiligo can have a major effect on quality of life, treatment can be considered and should preferably begin early when the disease is active. Current treatment modalities are directed towards stopping progression of the disease and achieving repigmentation. Therapies include corticosteroids, topical immunomodulators, photo(chemotherapy, surgery, combination therapies and depigmentation of normally pigmented skin. Topical class 3 corticosteroids can be used for localized vitiligo. The use of topical immunomodulators (TIMs in vitiligo seems to be equally effective as topical steroids, especially when used in the face and neck region. In photo(chemotherapy, narrowband ultraviolet-B therapy (NB-UVB seems to be superior to psoralen ultraviolet-A therapy (PUVA and broadband UVB. In surgical techniques, split-thickness grafting and epidermal blister grafting were shown to be effective methods, although the non-cultured epidermal suspension technique has many advantages and seems to be a promising development. Depigmentation therapy can be considered if vitiligo affects more than 60% to 80% of the body. Complementary therapies such as Polypodium leucotomos show promising results in combination with UVB therapy. No causative treatment for vitiligo is currently available. More randomized controlled trials on the treatment of vitiligo are necessary.Keywords: vitiligo, non-surgical treatment, surgical treatment

  13. Enhanced bleaching treatment: opportunities for immune-assisted melanocyte suicide in vitiligo.

    Science.gov (United States)

    Webb, Kirsten C; Eby, Jonathan M; Hariharan, Vidhya; Hernandez, Claudia; Luiten, Rosalie M; Le Poole, I Caroline

    2014-08-01

    Depigmentation in vitiligo occurs by progressive loss of melanocytes from the basal layer of the skin, and can be psychologically devastating to patients. T cell-mediated autoimmunity explains the progressive nature of this disease. Rather than being confronted with periods of rapid depigmentation and bouts of repigmentation, patients with long-standing, treatment-resistant vitiligo can undergo depigmentation treatment. The objective is to remove residual pigmentation to achieve a cosmetically acceptable result--that of skin with a uniform appearance. In the United States, only the use of mono-benzyl ether of hydroquinone (MBEH) is approved for this purpose. However, satisfactory results can take time to appear, and there is a risk of repigmentation. MBEH induces necrotic melanocyte death followed by a cytotoxic T-cell response to remaining, distant melanocytes. As cytotoxic T-cell responses are instrumental to depigmentation, we propose that combining MBEH with immune adjuvant therapies will accelerate immune-mediated melanocyte destruction to achieve faster, more definitive depigmentation than with MBEH alone. As Toll-like Receptor (TLR) agonists--imiquimod, CpG, and Heat Shock Protein 70 (HSP 70)--all support powerful Th1 responses, we propose that using MBEH in combination with these agents can achieve superior depigmentation results for vitiligo patients.

  14. Clinical analysis of 396 cases of vitiligo in childhood%儿童白癜风396例临床分析

    Institute of Scientific and Technical Information of China (English)

    黄博; 许爱娥; 卢良君; 陈梅花; 常淑彪

    2008-01-01

    Objective To determine the clinical features of childhood vitiligo in Han nationality.Methods A standardized questionnaire was used to clinically investigate 396 cases of vitiligo in patients aged less than 12 years;825 adult patients with vitiligo served as controls.Data analysis was done by SPSS 10.0.Results In child patients with vitiligo,46.2% were males,and 53.8%females;there was no statistically significant difference in gender ratio between the childhood and adult groups(P<0.05).The incidence of segmental vitiligo was 33.3%in children,significantly higher than that in adult patients(13.5%).On the contrary,the incidence of generalized vitiligo was significantly lower in children han in adult patients(4.3%vs 11.6%).A family history of vitiligo was observed in 12.4%of children,and in 12.7% of adults,withoutsignificant difference between the two groups.For both childhood and adult vitiligo patients,the incidence of vitiligo was significantly higher in firSt-degree relatives than in second-degree relatives.The most common precipitating factor of childhood vitiligo was trauma.The incidence of halo nevi was higher in patients with childhood vitiligo compared to patients with adult vitiligo,while thyroid disease was seen more frequently in adult patients.Conclusion There are some differences in clinical characteristics of vitiligo between children and adults.%目的 探讨汉族儿童白癜风的临床特点.方法 采用问卷调查方式对浙江省396例儿童白癜风及825例成人白癜风患者进行临床分析,用SPSS 10.0软件包对资料进行分析.结果 396例儿童白癜风患者中男183例占46.2%,女213例占53.8%,与成年患者相比,男女之间无性别差异.儿童患者中节段型的比例为33.3%,明显高于成人患者的13.5%,泛发型比例为4.3%,显著低于成人患者的11.6%.儿童患者家族史阳性比例为12.4%,成人患者家族史阳性比例为12.7%,两组间差异无统计学意义.有家族史患者一级亲属的发

  15. [Usefulness of corrective make-up in children with vitiligo coordinated by dermatology nursing].

    Science.gov (United States)

    Padilla-España, Laura; Ramírez-López, Belén; Fernández-Sánchez, Encarnación

    2014-01-01

    There are certain skin disorders such as vitiligo, acne, vascular malformations and postoperative scars that can affect the quality of life of children and especially adolescents. It can become an obstacle to their psychosocial development. A review was conducted on 4 patients with vitiligo located on face, who took part in a camouflage treatment course from January to December 2012. The impact of the skin disorder on quality of life was assessed before and after the therapeutic make-up sessions. Corrective makeup can be a complementary, reproducible, cost-effective, non-invasive, and useful technique in the management of dermatological diseases that have a physical and emotional impact in childhood. Copyright © 2013 Elsevier España, S.L. All rights reserved.

  16. Can systemically generated reactive oxygen species help to monitor disease activity in generalized vitiligo? A pilot study

    Directory of Open Access Journals (Sweden)

    Richeek Pradhan

    2014-01-01

    Full Text Available Background: Generalized vitiligo is a disease with unpredictable bursts of activity, goal of treatment during the active phase being to stabilize the lesions. This emphasizes the need for a prospective marker for monitoring disease activity to help decide the duration of therapy. Aims and Objectives: In the present study, we examined whether reactive oxygen species (ROS generated in erythrocytes can be translated into a marker of activity in vitiligo. Materials and Methods: Level of intracellular ROS was measured flow cytometrically in erythrocytes from venous blood of 21 patients with generalized vitiligo and 21 healthy volunteers using the probe dichlorodihydrofluorescein diacetate. Results: The levels of ROS differed significantly between patients and healthy controls, as well as between active versus stable disease groups. In the active disease group, ROS levels were significantly lower in those being treated with systemic steroids than those that were not. ROS levels poorly correlated with disease duration or body surface area involved. Conclusion: A long-term study based on these findings can be conducted to further validate the potential role of ROS in monitoring disease activity vitiligo.

  17. 白癜风治疗指南文献分析%Guidelines for the treatment of vitiligo: a review of literature

    Institute of Scientific and Technical Information of China (English)

    李阳; 许爱娥

    2012-01-01

    Vitiligo is a common disease characterized by hypopigmentation of skin and mucous membrane.It is easy to diagnosis,but difficult to treat.Recently,many new strategies have emerged at home and abroad for the treatment of vitiligo with the main purpose to control the progress of vitiligo and restore the color of lesions.Up to now,guidelines have been developed for the treatment of vitiligo in England,America,Holland,Saudi Arabia,Greece,China,and so on.The choice of vitiligo treatment options depends on culture background and clinical manifestations of patients.The authors review and compare the guidelines for vitiligo treatment from different countries,in hope to offer a reference for the treatment of vitiligo.%白癜风是一种常见的色素脱失性疾病,以皮肤或黏膜色素减退为特征,易诊而难治.近年来,国内外对其治疗方案很多,其目的主要是控制白斑的进展,使白斑复色直至正常的皮肤.目前英国、美国、荷兰、沙特阿拉伯、希腊、中国等国家已有针对白癜风的治疗指南.但是白癜风治疗方案的选择受不同文化背景和疾病临床特征的影响.概述国内外有关白癜风的治疗指南及不同国家的治疗指南做一简要比较,为白癜风患者的治疗提供参考方案.

  18. Follicular unit extraction as a therapeutic option for Vitiligo

    Directory of Open Access Journals (Sweden)

    S Sacchidanand

    2013-01-01

    Full Text Available Follicular unit extraction (FUE is a surgical procedure, which can be used to transplant follicular units into vitiliginous areas. Such follicular unit transplant has been recently used to repigment stable vitiligo patches. FUE was done for a 12-year-old female with a stable vitiligo patch with leukotrichia on the eyebrow. Repigmentation was noted in 6 weeks and complete pigmentation seen at 12 weeks. Leukotrichia resolved over a period of 6 months. No recurrence was noted at the end of 6 months follow-up with excellent colour match. This case is presented to highlight the simplicity, safety and effectiveness of FUE in stable vitiligo patches with leukotrichia.

  19. Autologous Epidermal Grafting Using a Novel Negative Pressure Epidermal Harvesting System in a Case of Stable Vitiligo

    Science.gov (United States)

    Thirunavukkarasu, Vanathi; Navaneetha Krishnan, Paru Priyadarshini; Danasekaran, Nithya Gayathri Devi; Rajendrabose, Ratnavel

    2016-01-01

    Vitiligo is a common pigmentary disorder of the skin with a great amount of social stigma attached to it. Though various medical modalities are available for the treatment of stable vitiligo, surgical modality remains the treatment of choice for stable and localized vitiligo. The surgical options range from simple punch grafting to the recent epidermal harvesting methods using a negative pressure unit. Although successful use of multiple methods of epidermal grafting has been reported, most of them are cumbersome and time-consuming. The new automated epidermal harvesting system now commercially available involves a tool that applies both heat and suction concurrently to normal skin to induce epidermal micrografts. Hence it serves as a safe, quick and cost-effective method without anesthesia, with a very minimal downtime for healing and requires an optimal expertise. The duration of repigmentation seems to be faster and more uniform compared to other procedures. We would like to share our experience with the negative pressure epidermal harvesting method in a patient with stable vitiligo. PMID:28003945

  20. Serum IL-2, IL-6, IL-10 and TNF-α Levels in Patients with Vitiligo before and after Treatment%白癜风患者治疗前后血清IL-2、IL-6、IL-10和TNF-α水平

    Institute of Scientific and Technical Information of China (English)

    孙岩; 钱立

    2011-01-01

    目的:探讨白癜风患者治疗前后血清IL-2、IL-6、IL-10、和TNF-α水平及临床意义.方法:应用放射免疫分析法检测32例白癜风患者治疗前后血清IL-2、IL-6、IL-10和TNF-α水平,以35名健康体检者为对照.结果:白癜风患者在治疗前血清IL-6、IL-10、TNF-α水平非常显著地高于对照组(P<0.01),IL-2水平非常显著地低于对照组(P<0.01);经治疗3个月后与对照组比较仍有显著性差异(P<0.05),且血清IL-2水平与IL-6、IL-10、TNF-α水平呈负相关(r=-0.482 2、-0.501 8、-0.613 4.P<0.01).结论:白癜风患者存在自身免疫调节的异常,检测血清IL-2、IL-6、IL-10和TNF-α水平的变化对疾病的治疗和预后具有重要的临床价值.%Objective: To detect serum levels of IL-2, IL-6, IL-10, and TNF-a in patients with vitili-go and to investigate its clinical significance. Methods:Radioimmunoassay was adopted to detect serum levels of IL-2, IL-6, IL-10 and TNF-α of 32 patients with vitiligo before and after treatment. The results were compared with those of 35 healthy people. Results: Serum levels of IL-6, IL-10, TNF-a in vitiligo patients before treatment were significantly higher than those of healthy people ( P<0.01) , while IL-2 level was significantly lower than that of healthy people (P<0.01). After treatment for 3 months, the differences between the two groups were significant (P<0. 05 ) , and serum IL-2 level was negatively correlated with IL-6, IL-10 and TNF-a levels (r=-0.482 2, -0.5018, -0.613 4.P <0.01). Conclusion: Autoimmune regulation is abnormal in vitiligo patients. Detection of serum IL-2, IL-6, IL-10, IL-6 and TNF-a levels have important clinical value in treatment and prognosis of the disease.

  1. Treatment with low-dose cytokines reduces oxidative-mediated injury in perilesional keratinocytes from vitiligo skin.

    Science.gov (United States)

    Barygina, Victoria; Becatti, Matteo; Lotti, Torello; Moretti, Silvia; Taddei, Niccolò; Fiorillo, Claudia

    2015-08-01

    Vitiligo is a systemic dermatological disorder characterized by the loss of skin pigmentation due to melanocyte injury or aberrant functioning. Recent data underline its multifactorial etiology with significant involvement of autoimmune and redox alterations. The major role in vitiligo cellular immunity is displayed by augmented Th1 and Th17 and suppressed TREGs and Th2 lymphocyte populations. Our previous studies indicate a marked redox imbalance in perilesional ("PL", i.e. obtained from visibly unaffected skin surrounding the depigmented area in vitiligo patients) keratinocytes where the massive infiltration of inflammatory cells takes place. No defined therapy exists for vitiligo. Although a number of approaches have been used for the induction of TREGs and Th2 cells, they may be associated with significant off-target effects. In order to identify a targeted approach for vitiligo treatment we, first, aimed to investigate the possible source of ROS overproduction in PL keratinocytes. Second, we tested the effect of low-dose selected cytokines, on intra- and extracellular ROS production, cell viability and cell cycle of PL keratinocytes. The in vitro study was conducted on primary PL keratinocytes obtained from the skin of vitiligo patients in our previous studies. The activity of NADPH oxidase was measured on intact PL and control keratinocytes, treated or not with cytokines, by luminometric assay. The following cytokines were selected for PL keratinocytes treatment: IL-10 and IL-4 (produced by TREGs and Th2, respectively), basic fibroblasts growth factor (bFGF) and neuropeptide β-endorphin (modulating the cellular resistance to oxidative stress and the immune response, respectively). All cytokines were used at concentration of 10fg/ml and were prepared by sequential-kinetic-activation (SKA). Intracellular ROS production and cell cycle were analyzed by flow cytometry using H2DCFDA and propidium iodide dyes, respectively. Cell viability was measured by

  2. CD207在白癜风发病机制中的作用研究%The role of CD207 in the pathogenesis of vitiligo

    Institute of Scientific and Technical Information of China (English)

    徐武生; 张江安; 于建斌; 刘冬冬; 李税琪; 郑良娟; 王绘霞

    2014-01-01

    目的:观察白癜风皮损中CD207的表达,探讨白癜风的免疫发病机制。方法采用免疫组织化学法检测12例节段型白癜风、25例寻常型白癜风和10例正常皮肤组织中CD207的表达水平,并比较其差异性。结果节段型白癜风组、寻常型白癜风组表皮内CD207表达分别为(61.2±10.9)和(64.3±12.5)个/mm,均高于正常对照组(52.4±10.3)个/mm,差异有统计学意义(P﹤0.05)。节段型白癜风组与寻常型白癜风组相比,CD207表达差异无统计学意义( P﹥0.05)。结论节段型白癜风、寻常型白癜风皮损组织中CD207的表达高于正常对照组,提示其发病机制与朗格汉斯细胞参与的细胞免疫反应有关。%Objective To observe the expression of CD207 in the lesions of vitiligo and the immune pathogenesis of vitiligo. Meth-ods The CD207 expression level was detected by immunohistochemistry in 12 patients with segmental vitiligo,25 patients with vitiligo vulgaris and 10 normal skin tissues,and the results were compared. Re-sults The expression of CD207 in segmental vitiligo group and vitiligo vulgaris group was(61. 2 ± 10. 9) and(64. 3 ± 12. 5)count/mm respectively,(52. 4 ± 10. 3)count/mm in control group. The differences were statistically significant( P﹤0. 05 ). Conclusions The expression of CD207 in the lesions of seg-mental vitiligo and vitiligo vulgaris is higher than that in the normal control group,indicating that the mechanism of vitiligo is associated with the cellular immunity response which langerhans cell was in-volved.

  3. Observation of roof of suction blister from normal skin of patients with vitiligo by Masson-Fontana stain%正常皮肤负压吸疱疱顶Masson Fontana染色分析

    Institute of Scientific and Technical Information of China (English)

    康尔恂; 毛玲娥; 罗邦国; 郑捷

    2001-01-01

    为研究负压吸疱法表皮移植治疗白癜风的机理,对16例来自白癜风病人色素正常部位皮肤负压吸引产生水疱的 疱顶组织进行HE和MassonFontana染色,观察水疱的位置及有无黑素细胞的存在。结果负压吸引产生的水疱位于棘细胞 层,无黑素细胞存在。治疗白癜风表皮移植的机理黑素细胞被移植这一假说不能成立。%To study the mechanism of treating vitiligo by epidermaltransplantation, the roof of suction blister from normal skin of 16 cases of vitiligo with HE and Masson-Fontana stain was observed to study the position of the vesicles and the presence of melanocytes in the roof of suction blisters、 The results showed that the vesicles were located in the spinous layer and no melanocytes were found. lt is concluded that the hypothesis that in epidermal transplantation melanocytes are transplanted is untenable.

  4. A Rare Colocalization of Lichen Planus and Vitiligo

    Directory of Open Access Journals (Sweden)

    David Veitch

    2015-01-01

    Full Text Available We report an unusual manifestation of vitiligo colocalizing with lichen planus (LP. A 76-year-old Greek male presented with a history of a red, scaly, itchy, asymmetrical patch located at the umbilicus within a well-demarcated depigmented macule of vitiligo. Histology showed features of a lichenoid interface dermatitis, favouring a diagnosis of LP. Colocalization of LP and vitiligo has rarely been reported in the literature. After reviewing the literature, we believe that at present there is insufficient evidence to resolve the uncertainties in the aetiology of this colocalization. It seems to us that the association between LP and vitiligo is more than coincidental, but none of the theories discussed in this paper can sufficiently account for it. Rather, the association is likely to be multifactorial in its pathogenesis.

  5. Goodbye warts, hello vitiligo: Candida antigen-induced depigmentation.

    Science.gov (United States)

    Wilmer, Erin N; Burkhart, Craig N; Morrell, Dean S

    2013-01-01

    Depigmentation after the use of topical immune modulators is a rare but reported event. Herein we present what is to our knowledge the first case of vitiligo at a site of Candida antigen injection. © 2012 Wiley Periodicals, Inc.

  6. 白癜风表皮黑素细胞及真皮噬黑素细胞的研究%Investigation of epidermal melanocytes and dermal melanophages in the vitiligo

    Institute of Scientific and Technical Information of China (English)

    李税琪; 李庆研; 刘冬冬; 徐武生; 李虎羽; 于建斌

    2014-01-01

    Objective To observe the changes of epidermal melanocytes and dermal melanoph-ages in vitiligo and investigate its significance. Methods Thirty-eight cases of vitiligo were divided into progressive stage group,stable stage group in nonsegmental vitiligo patients,and progressive stage group, stable stage group in segmental vitiligo patients. Normal controls of 10 cases were selected from the plas-tic surgery deparment in the same period. Melan-A positive cells and dermal melanophages were deter-mined by immunohistochemical staining and HE staining. Results Compared with normal controls,Me-lan-A positive cells counts were apparently lower and melanophages counts were higher in each vitiligo groups than those of normal controls,the differences were significant(P 0. 05). Melanophages counts of each vitiligo groups in pro-gressive stage were significantly higher than those of stable stage,the differences were significant(P 0.05)。同类型进展期组噬黑素细胞高于稳定期组,差异有统计学意义(P <0.01)。结论白癜风患者皮损中表皮黑素细胞明显减少,真皮噬黑素细胞明显增多,可能存在黑素细胞的真皮丢失途径。

  7. Effect of narrow band ultraviolet B phototherapy as monotherapy or combination therapy for vitiligo: a meta-analysis.

    Science.gov (United States)

    Li, Ronghua; Qiao, Meng; Wang, Xiaoyan; Zhao, Xintong; Sun, Qing

    2017-01-01

    The treatment of vitiligo is still one of the most difficult dermatological challenges, although there are many therapeutic options. Narrow band ultraviolet B (NB-UVB) phototherapy is considered to be a very important modality for generalized vitiligo. The aim of this study was to explore whether a combination of NB-UVB and topical agents would be superior to NB-UVB alone for treating vitiligo. We searched the electronic databases such as PUBMED, EMBASE, Cochrane Library, and Web of Science. The primary outcome was the proportion of ≥50% repigmentation (a clinical significance), and secondary outcome was the proportion of ≥75% repigmentation (an excellent response). Seven randomized controlled trials (RCTs) involving 240 patients (413 lesions) were included in this meta-analysis. The study showed no significant difference between NB-UVB combination therapy (NB-UVB and topical calcineurin inhibitor or vitamin D analogs) and NB-UVB monotherapy in the outcomes of ≥50% repigmentation and ≥75% repigmentation. However, lesions located on the face and neck had better results in ≥50% repigmentation (RR = 1.40, 95% CI 1.08-1.81) and ≥75% repigmentation (RR = 1.88, 95% CI 1.10-3.20) with NB-UVB and topical calcineurin inhibitor combination therapy vs. NB-UVB monotherapy. The meta-analysis suggested that adding neither topical calcineurin inhibitors nor topical vitamin-D3 analogs on NB-UVB can yield significantly superior outcomes than NB-UVB monotherapy for treatment of vitiligo. However, addition of topical calcineurin inhibitors to NB-UVB may increase treatment outcomes in vitiligo affecting face and neck. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  8. Determination of endothelin and stem cell factors in epidermal tissue fluid at the donor site and recipient site in patients with vitiligo receiving autologous epidermal grafting%白癜风自体表皮移植处内皮素与干细胞因子的检测

    Institute of Scientific and Technical Information of China (English)

    陈惠英; 关翠萍; 郑冬娣; 樊奇敏; 许爱娥

    2008-01-01

    Objective To investigate the relationship between the efficacy of autologous epidermal grafting and the levels of epidermal cytokines in vitiligo. Methods A total of 57 patients with stable vitiligo receiving autologous epidermal grafting were included in this study. Before grafting, 17 patients were irradiated with narrow-band UVB on vitiliginous sites. Suction blister fluid was collected from the recipient site (vitiligous lesions) and donor site (normal skin) in these patients (including the 17 patients irradiated with NB-UVB). ELISA was used to detect the levels of endothelin-1 (ET-1 ) and stem cell factors (SCF) in suction blister fluid. Clinical efficacy was evaluated through a 3-month follow-up. Resttlts Among these 57 patients, 45 successfully responded to autologous epidermal grafting. In these 45 patients, the levels of ET-1 and SCF in vitiligous lesions were 728.97±286.12 ng/L and 329.97±114.13 ng/L respectively, significantly higher than those in nomal skin (503.16±251.44 ng/L, 224.73±107.91 ng/L, t = 5.443, 5.897, respectively, both P 0.05). In those patients who responded successfully, the level of ET-1 was lower in the 15 patients exposed to NB-UVB compared to the other 30 patients without exposure (548.48±230.22 ng/L vs 794.60±278.72 ng/L, P0.05.在移植成功者中,行紫外线照射的15例内皮素-1为(548.48±230.22)ng/L,未照光组为(794.60±278.72)ng/L(P<0.05);干细胞因子浓度差异则无统计学意义. 结论 内皮素-1、干细胞因子在白癜风的色素恢复中起作用,其中内皮素-1的作用可能更为重要.

  9. A multicentric observational study to evaluate the role of homoeopathic therapy in vitiligo

    Directory of Open Access Journals (Sweden)

    P S Chakraborty

    2015-01-01

    Results: The changes in the mean VSS at intervals of every 6 months was found to be statistically significant. Homoeopathic treatment was found to be useful in relieving vitiligo in varying degrees in 126 patients, out of which 4 (2.94% cases showed marked improvement, 15 (11.03% cases showed moderate improvement, 77 (56.62% cases showed mild improvement, and 30 patients although improved, fell in the category of not significant improvement group (below 25% improvement. Ten homoeopathic medicines were found useful in the study of which Sulphur (n = 27, Arsenicum album (n = 19, Phosphorus (n = 19, and Lycopodium clavatum (n = 10 were the most commonly indicated and useful medicines.

  10. Meshed split skin graft for extensive vitiligo

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    Srinivas C

    2004-05-01

    Full Text Available A 30 year old female presented with generalized stable vitiligo involving large areas of the body. Since large areas were to be treated it was decided to do meshed split skin graft. A phototoxic blister over recipient site was induced by applying 8 MOP solution followed by exposure to UVA. The split skin graft was harvested from donor area by Padgett dermatome which was meshed by an ampligreffe to increase the size of the graft by 4 times. Significant pigmentation of the depigmented skin was seen after 5 months. This procedure helps to cover large recipient areas, when pigmented donor skin is limited with minimal risk of scarring. Phototoxic blister enables easy separation of epidermis thus saving time required for dermabrasion from recipient site.

  11. A systematic review of natural health product treatment for vitiligo

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    Boon Heather S

    2008-05-01

    Full Text Available Abstract Background Vitiligo is a hypopigmentation disorder affecting 1 to 4% of the world population. Fifty percent of cases appear before the age of 20 years old, and the disfigurement results in psychiatric morbidity in 16 to 35% of those affected. Methods Our objective was to complete a comprehensive, systematic review of the published scientific literature to identify natural health products (NHP such as vitamins, herbs and other supplements that may have efficacy in the treatment of vitiligo. We searched eight databases including MEDLINE and EMBASE for vitiligo, leucoderma, and various NHP terms. Prospective controlled clinical human trials were identified and assessed for quality. Results Fifteen clinical trials were identified, and organized into four categories based on the NHP used for treatment. 1 L-phenylalanine monotherapy was assessed in one trial, and as an adjuvant to phototherapy in three trials. All reported beneficial effects. 2 Three clinical trials utilized different traditional Chinese medicine products. Although each traditional Chinese medicine trial reported benefit in the active groups, the quality of the trials was poor. 3 Six trials investigated the use of plants in the treatment of vitiligo, four using plants as photosensitizing agents. The studies provide weak evidence that photosensitizing plants can be effective in conjunction with phototherapy, and moderate evidence that Ginkgo biloba monotherapy can be useful for vitiligo. 4 Two clinical trials investigated the use of vitamins in the therapy of vitiligo. One tested oral cobalamin with folic acid, and found no significant improvement over control. Another trial combined vitamin E with phototherapy and reported significantly better repigmentation over phototherapy only. It was not possible to pool the data from any studies for meta-analytic purposes due to the wide difference in outcome measures and poor quality ofreporting. Conclusion Reports investigating the

  12. The effectiveness of excimer laser on vitiligo treatment in comparison with a combination therapy of Excimer laser and tacrolimus in an Iranian population.

    Science.gov (United States)

    Matin, Marzieh; Latifi, Sahar; Zoufan, Nasrin; Koushki, Davood; Mirjafari Daryasari, Seyedeh Adeleh; Rahdari, Fereshteh

    2014-10-01

    Usage of 308-nm excimer laser (EL) is an effective treatment in vitiligo. As genetic predispositions along with type of skin and rate of sun exposure are known factors influencing the treatment outcomes, we tried to evaluate the treatment results of using 308 nm-excimer laser alone in comparison with the combined therapy of EL and topical tacrolimus in Iranian patients with vitiligo. We reviewed the medical files of 150 patients with vitiligo who were referred to the Behsima Laser Center between April 2012 and April 2013. Seventy five patients who received combined therapy of 308 nm EL and topical tacrolimus three times a week entered the study. Seventy-five controls with matched characteristics and who received only EL were also selected. The amount of repigmentation was estimated by an expert dermatologist and was classified in 5 ranks. In the case group, 33.3% (n: 25) showed 50-75% repigmentaion and 49.3% (n:37) had more than 75% response to therapy, whereas among the patients in the control group 29.3% (n: 22) showed no repigmentation and only 8% (n: 6) demonstrated more than 75% repigmentation response. The higher efficiency of the combination therapy on repigmentation was statistically significant (P: 0.006). Our study shows that Iranian patients with vitiligo who received a combined treatment course with 308-nm EL and tacrolimus 0.1% experienced significantly higher levels of repigmentation in comparison with laser therapy alone.

  13. Bullous contact dermatitis caused by self-applied crushed Paederus fuscipes for the treatment of vitiligo.

    Science.gov (United States)

    You, Dong-O; Kang, Jung-Dae; Youn, Nyoung-Hoon; Park, Seok-Don

    2003-11-01

    Paederus dermatitis is a linear, blistering contact dermatitis caused by pederin, a potent vesicant agent that is contained in insects belonging to the genus Paederus. This form of dermatitis usually occurs accidentally in those who have contact with this insect during the summer season. We report a peculiar case of a patient developing severe chemical burnlike lesions after application to her skin of many crushed Paederus fuscipes that she collected from the soil of a riverbank in the early spring for the treatment of her vitiligo.

  14. A Study of Noncultured Extracted Hair Follicle Outer Root Sheath Cell Suspension for Transplantation in Vitiligo

    Science.gov (United States)

    Shah, Aarti N; Marfatia, Ritu K; Saikia, Siddhartha S

    2016-01-01

    Context: Vitiligo surgeries have come a long way from tissue grafts to cultured and non cultured cell transplantation. Extracted hair follicle outer root sheath cell transplantation (EHF ORS) suspension is more enriched with melanocyte. In a hair bulb, there is one melanocyte for every five keratinocytes which is much higher than the epidermal melanin unit. Aims: To analyse the effectiveness of cultured EHF ORS and to perform objective evaluation based on clinical improvement & photographic evidence. To observe any untoward events or side effects. Settings and Design: The study was open and uncontrolled. All the patients were screened at preliminary visit. Reviews were done every two weeks. The endpoint selected was six months post procedure. Materials and Methods: Twenty five patients of stable Vitiligo were included in the study and follicular unit were harvested by Follicular Unit Extraction method. Outer root sheath cells were extracted by trypsinization. The solution was transplanted over dermabraded recipient site. Pressure dressing was given. Patients were followed up regularly. Statistical Analysis Used: Descriptive Statistics, Chi-Square. Results: Mean ± SD repigmentation was 80.15% ± 22.9% with excellent repigmentation (90-100%) in 60% of patients. Conclusions: This method is safe, effective, and simpler than the other methods involving cell culturing and requiring a laboratory set-up but selection of patients is crucial for the success of the outcome. PMID:27601859

  15. A Practical Approach to the Diagnosis and Treatment of Vitiligo in Children.

    Science.gov (United States)

    Ezzedine, Khaled; Silverberg, Nanette

    2016-07-01

    Vitiligo is a common inflammatory skin disease with a worldwide prevalence of 0.5% to 2.0% of the population. In the pediatric population, the exact prevalence of vitiligo is unknown, although many studies state that most cases of vitiligo are acquired early in life. The disease is disfiguring, with a major psychological impact on children and their parents. Half of vitiligo cases have a childhood onset, needing thus a treatment approach that will minimize treatment side effects while avoiding psychological impacts. Management of vitiligo should take into account several factors, including extension, psychological impact, and possible associations with other autoimmune diseases. This review discusses the epidemiology of vitiligo and outlines the various clinical presentations associated with the disorder and their differential diagnosis. In addition, the pathophysiology and genetic determinants, the psychological impact of vitiligo, and management strategies are reviewed.

  16. Dermatology life quality index scores in vitiligo: Reliability and validity of the Tunisian version

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    Jalel Akrem

    2009-01-01

    Full Text Available Background: Vitiligo is an important skin disease that can alter individual self-image and thus have major impact on the quality of life. Aims: The objective of this study was to translate and to test the reliability and validity of the 10-item Dermatology Life Quality Index (DLQI questionnaire in Tunisian vitiliginous patients. Methods: Using a standard "forward-backward" translation procedure, the English language version of the questionnaire was translated into Persian (the Iranian official language by two bilinguals. Seventy patients with vitiligo attending the Department of Dermatology, Regional Hospital, Medenine, Tunisia, were enrolled in this study. The reliability and internal consistency of the questionnaire were assessed by Cronbach′s “ coefficient and Spearman′s correlation, respectively. Validity was performed using convergent validity. Results: In all, 70 people entered into the study. The mean age of respondents was 28.3 (SD=11.09 years. Scores on the DLQI ranged from 0 to 24 (mean±SD, 7.05±5 .13. Reliability analysis showed satisfactory result (Cronbach′s “ coefficient=0.77. There were no statistically significant differences between daily activity (DA and personal relationship (PR scale mean scores in generalized versus focal-segmental involvement in sufferers ( P=0.056, P=0.053, respectively. There were also strong differences between the mean scores of the PR scale with the involvement of covered only and covered/uncovered areas ( P =0.016 that was statistically significant in the second group. Conclusions: The study findings showed that the Tunisian version of the DLQI questionnaire has a good structural characteristic and is a reliable and valid instrument that can be used for measuring the effects of vitiligo on quality of life.

  17. 儿童白癜风1125例的临床特征与治疗总结%Analysis of Clinical Characteristics and Theraputic of Children Vitiligo

    Institute of Scientific and Technical Information of China (English)

    李舒丽; 李春英; 刘玲; 坚哲; 黄也; 石琼; 韦超; 李强; 李凯; 高天文

    2012-01-01

    Objective To summarize and analyze the clinical characteristics and treatment of 1125 cases of children vitiligo, and to provide information for early prevention and proper treatment. Methods A retrospective study was conducted on 1 125 children vitiligo diagnosed at the Department of Dermatology in Xijing Hospital. Results Of the 1 125 children, the ratio of boys and girls was 1.04:1,with a mean onset age of 7.03 years. The most frequent age of onset was between 5 to 9 years (62.04%).The mean duration of vitiligo was 15.32 months. Patients (12.09%) recalled family history of vitiligo. The onset or progression of disease usually occurred in summer and spring, 15.29% patients had precipitating factors such as trauma, stress, and solarization. The most common type of vitiligo was vitiligo vulgaris(86.31%),6.84% presented combined diseases, Halo nevi was present in 50 (4.44%) cases, who revealed an earlier onset of vitiligo. The treatment of children vitiligo included traditional Chinese drug, topical or systemic corticosteroids, immunomodulator, phototherapy and surgical therapy, mainly in combination. 54.93% children with vitiligo responded to treatment of one year. Conclusion The family vitiligo history, halo nevi association and oxidative stress are risk factors for vitiligo in children. Management is difficult as therapeutic options are restricted in children, desiring further research.%目的:通过对1125例儿童白癜风患者的临床特点和治疗进行总结,以期对白癜风的早期预防及治疗提供依据.方法:对1125例第四军医大学西京医院皮肤科确诊的儿童白癜风病例进行回顾性分析.结果:1125例白癜风患儿中,男女比1.04:1,平均始发年龄7.03岁,发病年龄以5~9岁最多见(62.04%),平均病程15.32月.227例(20.1%)有家族史,春夏季发病或病情加重者所占百分比高;15.29%患儿发病前有外伤、精神因素、日晒等诱因.临床表现以寻常型最常见(86.31%),6.84%伴

  18. Ultrastructural changes of epidermal calcium gradient and lipid lamellar membrane in depigmented skin lesions of vitiligo patients%白癜风皮损表皮钙离子梯度与角质层板层脂膜超微结构观察

    Institute of Scientific and Technical Information of China (English)

    刘子重; 雷铁池

    2016-01-01

    characteristic alternating electron-dense and electron-lucent pattern.Expanded intercellular space,fragmentation and lamellar separation were observed in the depigmented skin lesions from vitiligo patients,the bulbous regions of lipid lamellae were filled with electron-dense amorphous materials.Large clumps of calcimn precipitates were visualized in the stratum granulosum (SG) of normal skin,fine calcium precipitates and stage Ⅳ melanosomes were noted within the normal stratum basale (SB).In depigmented skin lesions of vitiligo,both the size and number of calcium precipitates in the SG were dramatically decreased.Melanosome was barely seen in the vitiligo SB.Conclusion Disrupted ultrastructure of SC lamellar membranes and disappearance of calcium gradient co-exist in the skin lesion of vitiligo,indicating that melanin in epidermis may play a role in formation of epidermal calcium gradient and maintenance of structural integrity of permeability barrier.

  19. Effect of topical calcineurin inhibitors as monotherapy or combined with phototherapy for vitiligo treatment: a meta-analysis.

    Science.gov (United States)

    Dang, Yu-Ping; Li, Qiang; Shi, Fei; Yuan, Xiao-Ying; Liu, Wei

    2016-01-01

    Vitiligo is a common skin disease for which immunomodulating calcineurin inhibitors have been considered reasonable treatment. We searched the MEDLINE, Embase, and Cochrane central register of controlled trials databases for articles published prior to September 2014. Thirteen studies were included in the meta-analysis. After pooling the trials, we concluded that calcineurin inhibitors showed a better therapeutic effect on vitiligo than placebo, according to lesion report (RR = 2.62, 95%CI, 1.39-4.93, p = 0.003) and patient report (RR = 1.42, 95%, 0.87-2.31, p = 0.157). Subgroup analysis was performed to determine whether the combination with phototherapy was a source of heterogeneity. The trial sequence analysis indicated that the results of combined therapy by lesion report were reliable and conclusive. However, in the patient report trials, the frequency of lesions on the hand and foot was higher, and the effect of combined therapy was still not significant. Calcineurin inhibitors showed a better therapeutic effect than placebo in the treatment of vitiligo with phototherapy. However, the typical UV-resistant sites (i.e., hand and foot) were still difficult to cure even with combined therapy. Because of concerns about photocarcinogenesis, the clinical application of combined therapy should be explored with caution.

  20. Treatment experience of 2 631 cases of vitiligo%白癜风2246例治疗体会

    Institute of Scientific and Technical Information of China (English)

    李淑娟

    2015-01-01

    目的:探讨四联疗法治疗白癜风的效果。方法:2012-2014年收治白癜风患者2246例,采用四联疗法进行治疗,观察治疗效果。结果:2246例患者中,临床治愈1123例,有效1112例,无效11例。结论:四联疗法治疗白癜风取得了满意的效果。%Objective:To explore the curative effect of quartet therapy in the treatment of vitiligo.Methods:2 631 cases of patients with vitiligo from 2012 to 2014 were treated with quartet therapy.We observed the therapeutic effect.Results:In 2 631 cases of patients,1 123 cases got clinical cure;1 112 cases were treated effectively;11 cases were invalid.Conclusion:Quartet therapy has achieved satisfactory curative effect in the treatment of vitiligo.

  1. Helium-neon laser irradiation stimulates migration and proliferation in melanocytes and induces repigmentation in segmental-type vitiligo.

    Science.gov (United States)

    Yu, Hsin-Su; Wu, Chieh-Shan; Yu, Chia-Li; Kao, Ying-Hsien; Chiou, Min-Hsi

    2003-01-01

    Low-energy helium-neon lasers (632.8 nm) have been employed in a variety of clinical treatments including vitiligo management. Light-mediated reaction to low-energy laser irradiation is referred to as biostimulation rather than a thermal effect. This study sought to determine the theoretical basis and clinical evidence for the effectiveness of helium-neon lasers in treating vitiligo. Cultured keratinocytes and fibroblasts were irradiated with 0.5-1.5 J per cm2 helium-neon laser radiation. The effects of the helium-neon laser on melanocyte growth and proliferation were investigated. The results of this in vitro study revealed a significant increase in basic fibroblast growth factor release from both keratinocytes and fibroblasts and a significant increase in nerve growth factor release from keratinocytes. Medium from helium-neon laser irradiated keratinocytes stimulated [3H]thymidine uptake and proliferation of cultured melanocytes. Furthermore, melanocyte migration was enhanced either directly by helium-neon laser irradiation or indirectly by the medium derived from helium-neon laser treated keratinocytes. Thirty patients with segmental-type vitiligo on the head and/or neck were enrolled in this study. Helium-neon laser light was administered locally at 3.0 J per cm2 with point stimulation once or twice weekly. The percentage of repigmented area was used for clinical evaluation of effectiveness. After an average of 16 treatment sessions, initial repigmentation was noticed. Marked repigmentation (>50%) was observed in 60% of patients with successive treatments. Basic fibroblast growth factor is a putative melanocyte growth factor, whereas nerve growth factor is a paracrine factor for melanocyte survival in the skin. Both nerve growth factor and basic fibroblast growth factor stimulate melanocyte migration. It is reasonable to propose that helium-neon laser irradiation clearly stimulates melanocyte migration and proliferation and mitogen release for melanocyte growth

  2. Blister roof grafting, cultured melanocytes transplantation and non-cultured epidermal cell suspension transplantation in treating stable vitiligo: A mutual self-control study.

    Science.gov (United States)

    Bao, Huaye; Hong, Weisong; Fu, Lifang; Wei, Xiaodong; Qian, Guopei; Xu, Aie

    2015-01-01

    To compare the efficacy of blister roof grafting (BG), cultured melanocytes transplantation (CMT) and non-cultured epidermal cell suspension transplantation (NCES) in the treatment of stable vitiligo. In each person of 83 vitiligo patients one vitiligo macule was selected and divided in three areas for separate treatment with BG, CMT and NCES in the same session. The results were evaluated 12-month post-surgery for the extent of repigmentation and color match. A satisfactory result (>50% repigmentation) was achieved in 92%, 82% and 81% of the 83 patients with the BG, CMT and NCES methods, respectively. Significant differences between the BG and CMT groups (p = 0.038), and between BG and NCES groups (p = 0.017) were observed, but not between the CMT and NCES groups (p = 0.986). The extent of repigmentation on the head neck and trunk was superior to that of the extremities by all the three methods. A difference in the time of onset of repigmentation was observed, with repigmentation first appearing after 10 days, 20-30 days and >30 days in the BG, CMT and NCES groups, respectively. All the three methods are safe and effective to treat vitiligo. Future studies with larger groups are warranted to confirm our results.

  3. Molecular Mechanisms of Innate Immune Inhibition by Non-Segmented Negative-Sense RNA Viruses

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    Chatterjee, Srirupa; Basler, Christopher F.; Amarasinghe, Gaya K.; Leung, Daisy W.

    2016-08-01

    The host innate immune system serves as the first line of defense against viral infections. Germline-encoded pattern recognition receptors detect molecular patterns associated with pathogens and activate innate immune responses. Of particular relevance to viral infections are those pattern recognition receptors that activate type I interferon responses, which establish an antiviral state. The order Mononegavirales is composed of viruses that possess single-stranded, non-segmented negative-sense (NNS) RNA genomes and are important human pathogens that consistently antagonize signaling related to type I interferon responses. NNS viruses have limited encoding capacity compared to many DNA viruses, and as a likely consequence, most open reading frames encode multifunctional viral proteins that interact with host factors in order to evade host cell defenses while promoting viral replication. In this review, we will discuss the molecular mechanisms of innate immune evasion by select NNS viruses. A greater understanding of these interactions will be critical in facilitating the development of effective therapeutics and viral countermeasures.

  4. Evaluation of the Efficacy of Topical Ethyl Vanillate in Enhancing the Effect of Narrow Band Ultraviolet B against Vitiligo: A Double Blind Randomized, Placebo-Controlled Clinical Trial

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    Mohammad Reza Namazi

    2015-11-01

    Full Text Available Background: Vitiligo is an acquired disease of skin that presents with depigmented patches due to lack of melanocytes in the epidermis. Accumulation of toxic free radicals like hydrogen peroxide in the epidermis may be responsible for melanocytes death. Since ethyl vanillate (vanillic acid ethyl ester is a strong hydrogen peroxide scavenger, it may be effective against vitiligo. This study was carried out to evaluate the effect of ethyl vanillate cream on vitiligo patients receiving phototherapy. Methods: A double-blind placebo-controlled clinical trial using ethyl vanillate cream 20% was performed on 30 cases of generalized stable vitiligo (randomly selected who were receiving phototherapy in the outpatient clinic of Faghihi Hospital (Shiraz, Iran. The patients randomly applied ethyl vanillate on an assigned lesion (left or right side of the body and placebo on the opposite side lesion (almost the same size and location twice a day for 3 months, while receiving a narrow band ultraviolet B (NB-UVB 2-3 times weekly. Photos were taken at the beginning of the trial and at the end of 4th, 8th, and 12th weeks. Then, images were compared with the photos from the beginning of the trial based on VASI score. Results: There was a significant change in pigmentation after applying ethyl vanillate compared with baseline in medication side (P=0.002, but no significant change in placebo side (P=0.066. Additionally, there was a significant difference between medication and placebo sides in pigmentation (P=0.005. Conclusion: Ethyl vanillate may serve as an adjunct therapy for the treatment of vitiligo, although changes in pigmentation are mild clinically.

  5. Vitiligo: How do oxidative stress-induced autoantigens trigger autoimmunity?

    Science.gov (United States)

    Xie, Heng; Zhou, Fubo; Liu, Ling; Zhu, Guannan; Li, Qiang; Li, Chunying; Gao, Tianwen

    2016-01-01

    Vitiligo is a common depigmentation disorder characterized by a loss of functional melanocytes and melanin from epidermis, in which the autoantigens and subsequent autoimmunity caused by oxidative stress play significant roles according to hypotheses. Various factors lead to reactive oxygen species (ROS) overproduction in the melanocytes of vitiligo: the exogenous and endogenous stimuli that cause ROS production, low levels of enzymatic and non-enzymatic antioxidants, disturbed antioxidant pathways and polymorphisms of ROS-associated genes. These factors synergistically contribute to the accumulation of ROS in melanocytes, finally leading to melanocyte damage and the production of autoantigens through the following ways: apoptosis, accumulation of misfolded peptides and cytokines induced by endoplasmic reticulum stress as well as the sustained unfolded protein response, and an 'eat me' signal for phagocytic cells triggered by calreticulin. Subsequently, autoantigens presentation and dendritic cells maturation occurred mediated by the release of antigen-containing exosomes, adenosine triphosphate and melanosomal autophagy. With the involvement of inducible heat shock protein 70, cellular immunity targeting autoantigens takes the essential place in the destruction of melanocytes, which eventually results in vitiligo. Several treatments, such as narrow band ultraviolet, quercetin and α-melanophore-stimulating hormone, are reported to be able to lower ROS thereby achieving repigmentation in vitiligo. In therapies targeting autoimmunity, restore of regulatory T cells is absorbing attention, in which narrow band ultraviolet also plays a role.

  6. Clinical analysis and experimental study of vitiligo%白癜风临床分析及实验研究

    Institute of Scientific and Technical Information of China (English)

    李春英; 高天文; 李强; 李志强; 王秋枫

    2001-01-01

    AIM To probe into the clinical features and the cause of vitiligo. METHODS Clinical data of 2008 patients with vitiligo were reviewed; anti-pigment cell IgG antibodies in the sera of 283 patients with vitiligo was detected by ELISA; anti-pigment cell IgG antibodies and IgM antibodies in the sera of 45 patients with vitiligo were detected by Immunohistological methods; cytotoxic experiment mediated by complement was studied with the sera of 25 patients with active vitiligo. RESULTS ① Of the total 2008 vitiligo patients, the male -to -female ratio was 1.06. The average age of the patients at visit was 23.2 years old. The average of the onset age was 20 years old. There were 6 patients who fell ill when they were born. Head and neck were the most involved locations. Unilateral type was of the most. 82.2% patients were in advancing stage when they came to see the doctor. 303 patients (15.0%) were accompanied with other diseases. 110 (5.5%) of them were accompanied with halo nevi.156 (7.8%) patients have family history. 337 (16.4%) patients confirmed provocated factors. ② Of the sera of 25 patients with active vitiligo, IgG antibody was detected in 19 (76%) patients and IgM was detected in 21 (84%) patients, which was significantly higher than that in patients with inactive disease (35%, 10%) or controls (10%, 0) (P<0.01). ③ Of the sera of 25 patients with active vitiligo, cytotoxity mediated by the complement was observed in the sera of 9 (36%) patients. No cytotoxity was observed in the sera of the normal controls and in the fibroblasts. CONCLUSION Congenital vitiligo has been noticed in a few patients. Further more, we highlight the important role of autoantibodies in the pathogenesis of vitiligo.%目的了解白癜风的临床特点,探讨其发病机制. 方法回顾分析2008例白癜风患者的临床资料;以酶联免疫吸附法检测283例白癜风患者血清中的抗黑素细胞IgG抗体;以免疫组织化学染色法检测45例白癜风患者血清

  7. Effects of exercising on ET-1 in plasma and tissue fluid of vitiligo patients undergoing medicament treatment%运动对药物治疗白癜风患者血浆及组织液内皮素-1的影响

    Institute of Scientific and Technical Information of China (English)

    刘福泉; 王松涛; 刘忠海

    2014-01-01

    In order to probe into the effects of exercising of different intensities combined with medicament treat-ment on the area of skin lesion areas and ET-1 in plasma and tissue fluid of vitiligo patients, the authors selected 45 vitiligo patients and divided them randomly into 3 groups:a calm control (pure medicament) group, a medium in-tensity exercising (medium intensity exercising + medicament treatment) group, and a high intensity exercising (high intensity exercising+medicament treatment) group (each group included 15 patients), let the patients in the medium intensity exercising group run for 1 hour per time, at an exercising intensity that maintained the heart rate at 120-140 beats/min, let the patients in the high intensity exercising group do a rope skipping exercise for accumula-tively 1 hour per day and maintain their average heat rate at 160-170 beats/min during exercising, let the patients in the two exercising groups exercise for totally 16 weeks, 5 days per week, drew the patients’ blood and tissue fluid before and after 16-week exercising intervention, measured ET-1 mass concentration by applying radioimmunoas-say, determined the changing of copper ions by measuring trace elements in plasma, checked the changing of collat-eral blood vessels and vessel diameters by means of extremity vascular microcirculation, and revealed the following findings: 1)exercising increased ET-1 mass concentration in plasma and tissue fluid significantly, the patients in the high intensity exercising group had the most significant increase;2)before and after 16-week exercising, the patients in calm control group and the two exercising groups had an increased number of serum copper ions, the patients in the calm control group had a significant increase, while the patients in the exercising groups had only a slight increase;3)long term regular exercising increased the patients’ collateral blood vessels and vessel diameters, boosted blood cir-culation;4)After the experiment

  8. 先天性白癜风73例临床特征分析与治疗总结%Clinical features and therapy of 73 cases of congenital vitiligo

    Institute of Scientific and Technical Information of China (English)

    韦超; 马翠玲; 高天文; 李春英

    2012-01-01

    目的:总结分析先天性白癜风患者的临床特点及治疗方法,比较先天性白癜风与儿童白癜风的异同点.方法:对本院2000年1月~2011年12月诊治的73例先天性白癜风患者的临床资料进行回顾性分析.结果:73例先天性白癜风患者男女比例为0.92:1.神经节段型占15.1%,病情处于进展期者占56.2%,伴发晕痣者3例,有阳性家族史者占21.9%,治疗后好转或稳定者占86.7%.结论:①白癜风并非完全后天发病,出生时即可发生;②日晒可能是一种诱发加重因素;③有阳性家族史者符合多基因遗传规律;④联合治疗有较好的效果.%Objective To investigate the clinical characteristics of congenital vitiligo and find out its effective treatment, compared with a control group of vitiligo in children. Methods The clinical data and therapeutic conditions of 73 patients diagnosed with congenital vitiligo from 2000 to 2011 were retrospectively analyzed. Results 73 cases of congenital vitiligo were evaluated. Males constituted 47.9% (35) of the group and female 52.1%(38). Segmental vitiligo was in 11 (15.1%) patients. Condition in progress was present in 41 (56.2%) patients. Family history of vitiligo was present in 16 (21.9%) patients. Halo nevus was showed in 3 patients. The proportion of patients with improved condition after treatment was 86.7%. Conclusion ①Vitiligo can onset while born, not all acquired. ②Solarization may be an aggressive factor. ③lt is accorded with polygene heredity rule in family vitiligo. ④Combination therapy has good effect.

  9. Estudo comparativo entre vitiligo, nevo halo e lúpus eritematoso vitiligóide por meio de métodos imunológicos, histológicos e imuno-histoquímicos Comparative study of vitiligo, halo nevus, and vitiligoid variant of lupus erythematosus by immunological, histological, and immunohistochemical methods

    Directory of Open Access Journals (Sweden)

    Luiz Gonzaga C. Souza Filho

    2005-04-01

    verify whether the depigmentation observed in the diverse clinical forms of lupus is due to post-inflammatory destruction or to specific immunological attack on melanocytes. METHODS: 1- Detection of melanocyte antibodies: by direct and indirect immunofluorescence on nevus and melanoma cells; 2- Cytotoxicity evaluation: study of the activity of NK cells against cultivated melanoma cells; 3- Histopathological study of melanocytes and melanin: histopathology with hematoxylin-eosin, Fontana- Masson, Dopa and Dopa + silver and S-100 protein test by immunoperoxidase. RESULTS: Vitiligo and halo nevus patients presented to antimelanocyte antibodies in 25% of cases. Patients with vitiligoid variant of lupus erythematous also presented these antibodies. The presence of risk factors favoring cellular cytotoxicity was demonstrated in vitiligo and/or halo nevus, as well as in the vitiligoid variant of lupus erythematous. Staining with Dopa + silver nitrate was superior to traditional staining and to S-100 protein to detect melanocytes and/or melanin in depigmented lesions of vitiligo and/or halo nevus and vitiligoid variant of lupus erythematous. CONCLUSION: The results confirm the existence of antimelanocyte antibodies in vitiligo and halo nevus. It is not possible to rule out some immunological phenomena similar to those occurring in vitiligo and halo nevus in the genesis of vitiligoid lesions in lupus erythematous. The detection of melanocytes in achromic lesions of vitiligo suggests the predominance of a functional inhibitory mechanism rather than cell destruction in the genesis of the disease.

  10. 黑素细胞移植治疗甲状腺功能异常白癜风的临床观察%Autologous melanocyte transplantation for the treatment of vitiligo in patients with abnormal thyroid function:a clinical observation

    Institute of Scientific and Technical Information of China (English)

    李伟; 洪为松; 傅丽芳; 尉晓冬; 许爱娥

    2014-01-01

    Objective To evaluate the therapeutic efficacy of autologous melanocyte transplantation for the treatment of vitiligo in patients with abnormal thyroid function.Methods A total of 60 patients with vitiligo were enrolled in this study,including 30 with abnormal thyroid function and 30 without.Epidermal sheets were obtained by suction blister biopsy from the normal skin of all the patients followed by melanocyte isolation and culture.After 2-5 passages of subculture,the melanocytes were transplanted onto vitiliginous lesions,which were abraded previously by ultra-pulsed CO2 laser,in the corresponding patients.All the patients were followed for 6-12 months.Results Of the 30 patients with abnormal thyroid function,7 patients achieved more than 90% repigmentation,9 patients 50%-89% repigmentation,53.3% more than 50% repigmentation,with the average repigmentation rate being 47% within 6 months after the transplantation.Meanwhile,13 out of the 30 patients without abnormal thyroid function showed more than 90% repigmentation,11 showed 50%-89% repigmentation,with the average repigmentation rate being 75%.Both the cure rate and response rate were significantly higher in the patients without abnormal thyroid function than in those with (cure rate,43.3% vs.23.3%,P< 0.05; response rate,80% vs.53.3%,P< 0.05).Significant differences were also found in the response rate for lesions on the face or neck and for those sized more than 20 cm2 between the two groups of patients (both P < 0.05).The lesions transplanted with epidermal melanocytes from the waist exhibited the lowest cure rate and response rate.Conclusion Clinical or subclinical thyroid dysfunction may have a negative impact on the efficacy of autologous melanocyte transplantation in vitiligo.%目的 探讨自体培养黑素细胞移植治疗甲状腺功能异常白癜风的临床疗效.方法 白癜风患者分为2组,甲状腺功能异常组30例和无甲状腺功能异常组30例.负压

  11. Clinical and immunological features of childhood vitiligo%儿童白癜风临床及免疫学研究

    Institute of Scientific and Technical Information of China (English)

    林晓; 傅雯雯

    2008-01-01

    目的 探讨儿童白癜风与免疫的关系.方法 采用统一设计的儿童白癜风临床特点调查表对本院门诊的14岁以下患儿及其家属进行调查.对270例患儿检测外周血免疫球蛋白、补体水平和T细胞亚群.结果 白癜风患儿620例中男302例(48.71%),女318例(51.29%),平均就诊年龄8.81岁,平均发病年龄7.57岁,平均病程8.14个月.累及头颈部453例,占73.06%.节段型160例,占25.81%.发病季节和疾病进展均以春夏季为多.73例患儿伴有晕痣,占11.77%,以散发型者居多.有家族史者84例,占13.55%.节段型患儿中,进展期的患儿血C3、c4水平明显低于稳定期患儿(P<0.05).寻常型患儿中,进展期的患儿血CD3+,CD4+细胞水平和CD4+/CD8+比值明显低于稳定期患儿(P<0.01).结论 白癜风患儿体内存在某些免疫指标的异常,可能与免疫紊乱有关.%Objective To discuss the relationship between immunity and childhood vitiligo. Methods A questionnaire was designed to investigate the clinical characteristic of patients younger than 14 years with vi-tiligo and their relatives. The serum levels of immunoglobulin, complement and T-lymphocyte subsets were de-tected in 270 cases of pediatric vitiligo. Results A total of 620 patients were investigated. Of them, 302(48.71%) were boys and 318 (51.29% ) were girls, with a mean age of 8.81 years and mean onset age at 7.57years. The mean disease duration was 8.14 months. The involvement of head and neck was observed in 453(73.06%) children. Segmental vitiligo was diagnosed in 160 (25.81%) children. Spring and summer are predilected seasons of pediatric vitiligo. Halo nevi were found in 73 (11.77%) children,and most in those with scattered vitiligo. Family history was observed in 84 (13.55%) children. A significant decrease of serum C3 and C4 was observed in patients with segmental vitiligo in active phase compared with those in stable phase (P < 0.05). Similarly, the count of CD4+ and CD3+ lymphocytes in

  12. Clinical analysis on 826 cases of vitiligo%白癜风826例临床分析

    Institute of Scientific and Technical Information of China (English)

    鲍迎秋; 傅裕; 常建民

    2009-01-01

    对1998年至2008年我院白癜风门诊就诊的826例患者的临床资料做回顾性分析.结果 示其发病时间主要在青少年期;有阳性家族史者占20.6%(170例),一、二级亲属共患率分别为44.1%(75例)和41.2%(70例),高于三级亲属;家族史阳性患者的平均发病年龄(25±16)岁,早于家族史阴性患者[(28±17)岁],两者差异有统计学意义(t=1.97,P<0.05).合并自身免疫性疾病的患者67例,占8.1%;儿童白癜风患者比其他年龄患者更易发生同形反应和出现晕痣(P<0.05).%We retrospectively reviewed the clinical data of 826 cases with vitiligo treated in Beijing Hospital from 1998 to 2008. Most of patients present vitiligo in early age;20. 6% patients had positive family history. There were 44. 12 %, 41.18% and 10. 00 % of patients with vitiligo in their first, second and third degree relatives, respectively. Patients with family history had an earlier onset than those without a family history ( t = 1.97, P < 0. 05 ). Vitiligo vulgaris was found in 91.40 % patients. The most common affected site was head and face (31.5%) ;8. 1% of the patients was associated with other autoimmune diseases, including thyroid diseases, diabetes. There were more females than males in patients aged below 14. The child patients were more likely to present with Koebner' sign and halo nevus than the elder ones (P<0.05).

  13. Depigmentation with Q-switched Nd:YAG laser in universal vitiligo: a long-term follow-up study of 4 years.

    Science.gov (United States)

    Majid, Imran; Imran, Saher

    2017-05-01

    Q-switched (QS) lasers are used for depigmentation in universal vitiligo, but there is limited data on long-term results after laser treatment. This study was conducted to assess the safety and long-term follow-up results of QS Nd:YAG laser treatment in combination with need-based topical depigmentation therapy for universal vitiligo. A retrospective study was performed on patients with universal vitiligo who had received QS Nd:YAG laser treatment from 2010 to 2013. All patients were contacted and called for follow-up. Patients who reported were assessed clinically and information regarding adverse effects and relapse (repigmentation) in the follow-up period were obtained. Any need for topical depigmenting creams or other interventions and overall satisfaction to treatment were also documented. Records of 34 patients were retrieved, out of whom 28 cases reported for the follow-up visit. Duration of follow-up ranged from 2 to 5 years (mean, 2.78). No long-term adverse effects were reported and the majority of patients were highly satisfied with treatment. Satisfactory results with >90% clearance of pigment was reported by 89.3% (25/28) of patients, out of whom 72% (18/25) had retained the therapeutic effect with the use of sunscreens with/without intermittent topical depigmentation therapies. In seven cases, partial to complete relapse was observed. QS Nd:YAG laser is an effective tool for treating residual pigmentation in universal vitiligo, and the therapeutic effect can be maintained in most cases with regular sunscreen use and need-based topical therapies.

  14. Herpes zoster on segmental vitiligo: Wolf’s isotopic response?

    Directory of Open Access Journals (Sweden)

    Mankesh Lal Gambhir

    2014-04-01

    Full Text Available “Wolf’s isotopic response” describes the occurrence of a new skin disorder at the site of another, unrelated and already healed skin disease. In most cases of isotopic response, the initial dermatosis is herpes zoster, herpes simplex, varicella, thrombophlebitis, scrofuloderma and striae distense. The most frequent second dermatoses are granulomatous reactions, particularly granuloma annulare, and lichenoid diseases. Various etiological reasons including viral, immunologic, neural and vascular have been put forth. We report here a case in which the second disease was herpes zoster that appeared over the same dermatomes of pre-existing segmental vitiligo. The occurrence of vitiligo as first and herpes zoster as second disease in the “Wolf’s isotopic response” has not, to the best of our knowledge, been reported previously.

  15. Advances in Vitiligo: An Update on Medical and Surgical Treatments

    Science.gov (United States)

    Dillon, Alexander B.; Sideris, Andrew; Hadi, Ali

    2017-01-01

    Vitiligo is one of the most common cutaneous disorders of depigmentation. Although its underlying causes are still being studied and no definitive cure currently exists, recent research has provided insight into pathogenic mechanisms and new treatment options. Objective: The aim of this paper is to provide a comprehensive overview of the medical and surgical therapies for vitiligo with emphasis on the most recent treatment modalities. Design: This review was conducted through a literature search using PubMed and the National institutes of Health’s clinicalTrials.gov databases from January 2010 to July 2015. This yielded 86 studies, 12 of which were excluded, and 74 of which were reviewed. Results: Recent studies and ongoing clinical trials indicate that there are many promising new medical and surgical treatment modalities for this chronic condition. Conclusion: A combination of traditional and newer treatments may work synergistically to provide additional improvement in patients’ disease state and quality of life.

  16. Squamous cell carcinoma complicating vitiligo in an Indian man

    Directory of Open Access Journals (Sweden)

    Amit Kumar Dhawan

    2012-01-01

    Full Text Available An elderly man, a known case of generalized vitiligo of long duration, presented to us with an ulcerated exophytic growth arising from the vitiliginous skin. The histopathological study confirmed the clinical suspicion of squamous cell carcinoma. Cutaneous neoplasia arising from the vitiliginous skin is a rare situation. Lack of melanin leaves the skin vulnerable to ultraviolet radiation damage, which may predispose to cutaneous neoplasia. Therefore, the importance of photoprotection has been stressed upon through this illustration.

  17. Possible contribution of GSTP1 and other xenobiotic metabolizing genes to vitiligo susceptibility.

    Science.gov (United States)

    Minashkin, Mikhail M; Salnikova, Lubov E; Lomonosov, Konstantin M; Korobko, Igor V; Tatarenko, Andrey O

    2013-04-01

    Vitiligo is an acquired pigmentary disorder with several proposed pathogenesis mechanisms and complex multifactorial genetic predisposition. We analyzed 65 polymorphisms in genes potentially relevant to vitiligo pathogenesis mechanism to reveal novel and confirm reported genetic risk factors in general Russian population. We found that polymorphism rs1138272 (TC + CC) in GSTP1 gene encoding enzyme involved in xenobiotic metabolism is associated with vitiligo (Bonferroni adjusted P value 0.0015) with extraordinary high odds ratio 13.03, and haplotype analysis confirmed association of GSTP1 gene with vitiligo risk. Moreover, analysis of variations in several genes encoding enzymes of xenobiotic metabolism showed that higher risk of vitiligo is associated with higher number of risk alleles. This finding reveals possible contribution of genetic background to observed imbalance of oxidative stress control in vitiligo through cumulative effect of multiple genetic variations in xenobiotic metabolizing genes, supporting the concept of multigenic nature of vitiligo with multiple low-risk alleles cumulatively contributing to vitiligo risk.

  18. Dysregulated autophagy increased melanocyte sensitivity to H2O2-induced oxidative stress in vitiligo

    Science.gov (United States)

    He, Yuanmin; Li, Shuli; Zhang, Weigang; Dai, Wei; Cui, Tingting; Wang, Gang; Gao, Tianwen; Li, Chunying

    2017-01-01

    In vitiligo, melanocytes are particularly vulnerable to oxidative stress owing to the pro-oxidant state generated during melanin synthesis and to the genetic antioxidant defects. Autophagy is a controlled self-digestion process which can protect cells against oxidative damage. However, the exact role of autophagy in vitiligo melanocytes in response to oxidative stress and the mechanism involved are still not clear. To determine the implications of autophagy for melanocyte survival in response to oxidative stress, we first detected the autophagic flux in normal melanocytes exposure to H2O2, and found that autophagy was significantly enhanced in normal melanocytes, for protecting cells against H2O2-induced oxidative damage. Nevertheless, vitiligo melanocytes exhibited dysregulated autophagy and hypersensitivity to H2O2-induced oxidative injury. In addition, we confirmed that the impairment of Nrf2-p62 pathway is responsible for the defects of autophagy in vitiligo melanocytes. Noteworthily, upregulation of the Nrf2-p62 pathway or p62 reduced H2O2-induced oxidative damage of vitiligo melanocytes. Therefore, our data demonstrated that dysregulated autophagy owing to the impairment of Nrf2-p62 pathway increase the sensitivity of vitiligo melanocytes to oxidative stress, thus promote the development of vitiligo. Upregulation of p62-dependent autophagy may be applied to vitiligo treatment in the future. PMID:28186139

  19. MOLECULAR HETEROGENEITY OF THE 4TH COMPONENT OF COMPLEMENT (C4) AND ITS GENES IN VITILIGO

    NARCIS (Netherlands)

    VENNEKER, GT; WESTERHOF, W; DEVRIES, IJ; DRAYER, NM; WOLTHERS, BG; DEWAAL, LP; BOS, JD; ASGHAR, SS

    1992-01-01

    In view of evidence suggesting vitiligo is an autoimmune disease, we investigated whether vitiligo is associated with inherited deficiencies of the fourth (C4) and second (C2) component of complement and with certain human leukocyte antigens (HLA). Analysis of functional activities of C4 and C2 in s

  20. Autologous miniature punch skin grafting in stable vitiligo

    Directory of Open Access Journals (Sweden)

    Savant S

    1992-01-01

    Full Text Available Autologous split thickness miniature punch skin grafting is one of the surgical modes of treatment of stable vitiligo. Out of 87 different sites, of stable vitiligo, occurring in 62 cases, (32 focal, 22 segmental and 8 generalised 75 sites showed total repigmentation with excellent cosmetic colour match. Out of the 62 cases, 46 cases who were treated postsurgically with PUVA therapy repigmented within 2 ½ to 3 months, 10 cases, who received no treatment postsurgically repigmented by 3 ½ to 6 months. In addition 6 cases in whom no treatment was given postsurgically had to be given PUVA therapy 3 months after surgery as there was poor repigmentation. The complications seen were graft rejection due to improper immobilization in 6 cases, graft rejection due to secondary infection in 1, contact allergic dermatitis to framycetin in 3, and reactivation of vitiligo in 2. Side effects seen were cobblestoning in 32, sinking pits in 12, variegated appearance in 4, and superficial scarring at donor site in all 62 cases.

  1. Promoter polymorphism -119C/G in MYG1 (C12orf10) gene is related to vitiligo susceptibility and Arg4Gln affects mitochondrial entrance of Myg1

    DEFF Research Database (Denmark)

    Philips, Mari-Anne; Kingo, Külli; Karelson, Maire;

    2010-01-01

    MYG1 (Melanocyte proliferating gene 1, also C12orf10 in human) is a ubiquitous nucleo-mitochondrial protein, involved in early developmental processes and in adult stress/illness conditions. We recently showed that MYG1 mRNA expression is elevated in the skin of vitiligo patients. Our aim was to ...

  2. Promoter polymorphism -119C/G in MYG1 (C12orf10) gene is related to vitiligo susceptibility and Arg4Gln affects mitochondrial entrance of Myg1

    DEFF Research Database (Denmark)

    Philips, Mari-Anne; Kingo, Külli; Karelson, Maire

    2010-01-01

    MYG1 (Melanocyte proliferating gene 1, also C12orf10 in human) is a ubiquitous nucleo-mitochondrial protein, involved in early developmental processes and in adult stress/illness conditions. We recently showed that MYG1 mRNA expression is elevated in the skin of vitiligo patients. Our aim...

  3. [A clinical constelation in disuse: vitiligo, mastocytosis of the bone marrow, circulating heparinoid, chronic leg ulcer, hypoalbuminemia, ulcerative colitis and hepatic fibrosis].

    Science.gov (United States)

    Goihman Yahr, M; Goldstein, C; González, Y; Aranzazu, N; Convit, J; Acevedo Gallegos, F; Grases, P

    1977-01-01

    We report an unusual case. Our patient had vitiligo, ulcerative colitis, hepatic fibrosis, hypoalbuminemia, bone marrow mastocytosis, a circulating heparinoid and chronic leg ulcers. The relationship between some of the components of this constellation seems logical. This is the case with bone marrow mastocytosis and circulating heparinoid. Also, between hypoalbuminemia and colitis and liver disfunction. On the other hand, the relationship between other features does not seem clear in the light of current knowledge.

  4. Photodynamic therapy with low-strength ALA, repeated applications and short contact periods (40-60 minutes) in acne, photoaging and vitiligo.

    Science.gov (United States)

    Serrano, Gabriel; Lorente, Matilde; Reyes, Madga; Millán, Fernando; Lloret, Adrián; Melendez, Joaquín; Navarro, Maria; Navarro, Miguel

    2009-06-01

    Topical aminolevulinic acid (ALA) photodynamic therapy (PDT) is currently being used for the treatment of actinic keratosis of the face and scalp. This study reports the results obtained after three to four treatments with ALA-PDT in patients with acne (n=12), photoaging (n=8) and vitiligo (n=6). ALA was applied on large areas (e.g., full face) and at very low strengths (1-2%). Side effects were minimal and self-limited.

  5. Polymorphism of the E-cadherin gene CDH1 is associated with susceptibility to vitiligo.

    Science.gov (United States)

    Tarlé, Roberto Gomes; Silva de Castro, Caio Cesar; do Nascimento, Liliane Machado; Mira, Marcelo Távora

    2015-04-01

    Vitiligo is a depigmenting disorder characterized by loss of functional melanocytes from the epidermis. Experimental data suggest that defective melanocyte adhesion may underlie the pathogenesis of the disease. In particular, association between vitiligo and genetic variants of the DDR1 gene involved in melanocyte adhesion has been recently published. A subsequent, independent study revealed lower expression of DDR1 in vitiligo lesions. Here, we expand this investigation by testing for association between vitiligo and polymorphisms of CDH1, IL1B and NOV (formerly CCN3), genes belonging to the DDR1 adhesion pathway, in two population samples of distinct design. Our results reveal that alleles of marker rs10431924 of the CDH1 gene are associated with vitiligo, especially in the presence of autoimmune comorbidities.

  6. Effect of 308 nm Excimer Laser on TNF-α,bFGF in the Skin Tissue Fluid of Patients with Vitiligo Vulgaris in Stable Stage%308 nm 准分子激光对寻常型稳定期白癜风患者皮肤组织液 TNF-α、bFGF 水平的影响

    Institute of Scientific and Technical Information of China (English)

    皮肖冰; 谢志红; 潘景良

    2014-01-01

    Objective:To evaluate the influence of the 308 nm excimer laser on the level of the tumor necrosis factor-α( TNF-α) and the basic fibroblastic growth factor ( bFGF) in the skin tissue fluid of patients with vitiligo vulgaris in stable stage .Methods:28 patients with vitiligo vulgaris in stable stage receiving autologous epidermal grafting were included in this study .The vitiliginous lesion , the perilesional skin and the normal skin were divided into laser treatment section and non-laser treatment section . Before grafting , the laser treatment section were irradiated with the 308 nm excimer laser for ten times .The suction blister fluid in different section were collected , and the level of TNF-αand bFGF of those fluids in different section were measured by the enzyme linked immunosorbent assay .Results:In vitiliginous lesion , perilesional skin and normal skin , the TNF-αlevel in the laser treatment section were lower than that in the non-laser treatment section , while the bFGF level in the laser treatment section were higher than that in the non-laser treatment section(P0.05).Conclusion:The 308 nm excimer laser can inhibit the keratinocyte to secret TNF-αand upgrade the level of bFGF in tissue fluids , which would be one of mechanisms of the 308 nm excimer laser in treatment of vitiligo on inducing repigmentation of skin lesions .%目的:评价308 nm 准分子激光对寻常型稳定期白癜风患者皮肤组织液肿瘤坏死因子( TNF-α)、碱性成纤维细胞生长因子( bFGF)水平的影响。方法:对28例寻常型稳定期白癜风患者进行负压吸疱表皮移植,先将白斑、围白斑、正常皮肤分为非激光区和激光区。在移植前,激光区接受308 nm准分子激光治疗10次;非激光区不作处理。吸疱移植时收集各区疱液,采用酶联免疫吸附法( ELISA)测定皮肤组织液中TNF-α和bFGF水平。结果:白斑、围白斑、正常皮肤的激光区分别与各自的非激光

  7. Treatment of 30 Cases of Vitiligo by Cupping Method plus External Application of Chinese Herbs

    Institute of Scientific and Technical Information of China (English)

    ZHANG Shu-qing; CHE Jie

    2004-01-01

    在皮损区拔罐,然后外涂中药治疗白癜风患者30例,对照组予西药治疗,3个疗程后,治疗组总有效率96.7%,对照组总有效率76.7%.治疗组疗效好于对照组(P<0.05).%Thirty patients with vitiligo were treated by cupping of skin lesion and compress of Chinese herbs, compared with western medical treatment. After 3-course's treatments the total effective rate ws 96.7% in treatment group and 76.7% in control group. There was a significant difference (P<0.05)between the two groups shown by statistical analysis.

  8. 临床特征和皮肤CT特征判定白癜风分期%Vitiligo staging based on clinical and skin computed tomography features

    Institute of Scientific and Technical Information of China (English)

    刘涛; 许爱娥

    2015-01-01

    目的 采用临床特征和皮肤CT特征来判定白癜风分期.方法 200例白癜风患者按照临床特征问卷和皮肤CT特征进行分期:>2分为快速进展期,1~2分为缓慢进展期,<1分为稳定期.选择进展期和稳定期白癜风患者各5例,在皮肤CT检测的区域进行HE染色分析.结果 用临床特征和皮肤CT特征判定200例白癜风患者的分期,差异无统计学意义.临床特征:进展期为白斑边缘隆起或与周围正常皮肤边界不清,三色白癜风,皮损颜色呈灰白色或浅白色;稳定期为白斑区与正常皮肤边界清晰,皮损颜色呈乳白色或瓷白色,可见色素岛.皮肤CT:进展期为表真皮交界处色素环失去完整性,与周边正常皮肤边界不清,在表真皮交界、边缘处可以看到高折光性细胞.稳定期为表真皮交界处色素环完全缺失,与周边正常皮肤边界清,有树突状高折射光的黑素细胞存在.HE染色结果显示,进展期在真皮乳头层内的病灶的边缘可见大量的CD8T淋巴细胞.稳定期在真皮乳头层内的病灶边缘未见CD8T淋巴细胞.结论 临床特征和皮肤CT特征可以用来判定白癜风的分期,结果与进展期组织病理学基本一致.%Objective To determine the stage of vitiligo according to clinical and skin computed tomography (CT) features.Methods The stage of vitiligo was determined in 200 patients according to a questionnaire-based analysis of clinical features and skin CT findings respectively.Rapid progressive stage was defined as the score for vitiligous lesions or CT findings being higher than 2,slow progressive stage as the score varying from 1 to 2,and stable stage as the score less than 1.Skin samples were obtained at lesional sites receiving CT examination from 5 patients with progressive and 5 patients with stable vitiligo,and subjected to hematoxylin-eosin (HE) staining.Results No significant difference was observed between staging results based on clinical features and

  9. 幽门螺杆菌感染与白癜风的关联性分析%Correlation analysis between helicobacter pyloriinfection and vitiligo

    Institute of Scientific and Technical Information of China (English)

    朱建萍; 黄小雄; 解士海

    2015-01-01

    目的:探讨幽门螺杆菌(Hp)感染与白癜风之间的联系。方法80例白癜风患者(观察组)与100例健康体检者(健康对照组),分别采用酶联免疫吸附试验法(ELISA)检测两组血清抗幽门螺杆菌血清抗体。结果观察组62例(77.5%)阳性,而健康对照组11例(11.0%)阳性,观察组Hp阳性率明显高于健康对照组(χ2=81.53, P0.05)。结论白癜风患者的血清Hp IgG抗体阳性率显著高于正常人群,提示幽门螺杆菌与部分白癜风的发病有一定关系。%Objective To investigate correlation between helicobacter pylori (Hp) infection and vitiligo. Methods There were 80 vitiligo patients (observation group) and 100 healthy people (healthy control group), and they received enzyme-linked immuno sorbent assay (ELISA) for detection of serum anti-helicobacter pylori antibody.Results There were 62 positive cases (77.5%) in the observation group and 11 positive cases (11.0%) in the healthy control group. The observation group had much higher Hp positive rate than the healthy control group (χ2=81.53,P0.05).Conclusion Vitiligo patients had higher positive rate of serum Hp IgG antibody than normal people. This can show correlation between helicobacter pylori and vitiligo.

  10. Clinical observation on treating 56 cases of vitiligo in the integrative medicine%中西医结合治疗皮肤白斑56例临床观察

    Institute of Scientific and Technical Information of China (English)

    郭秀霞

    2013-01-01

      目的:观察中药配合维生素B12治疗皮肤白斑的临床疗效。方法:用中药配合维生素B12注射液为56例皮肤白斑患者治疗,疗程3周。结果:采用中药配合维生素B12注射液治疗皮肤白斑患者,治愈率高,副作用少。结论:中药配合维生素B12注射液治疗皮肤白斑具有良好的临床应用效果。%Objective:To investigate the clinical effect of TCM combined with Vitamin B12 injection on treating vitiligo. Methods:56 patients of vitiligo were treated with TCM combined with Vitamin B12 injection. Results:The vitiligo patients treated with TCM and Vitamin B12 injection had high cure rate and little adverse effects. Conclusion: TCM combined with Vitamin B12 injection was significantly effective on vitiligo.

  11. Fluorescence excitation-emission matrix spectroscopy of vitiligo skin in vivo (Conference Presentation)

    Science.gov (United States)

    Zhao, Jianhua; Richer, Vincent; Al Jasser, Mohammed; Zandi, Soodabeh; Kollias, Nikiforos; Kalia, Sunil; Zeng, Haishan; Lui, Harvey

    2016-02-01

    Fluorescence signals depend on the intensity of the exciting light, the absorption properties of the constituent molecules, and the efficiency with which the absorbed photons are converted to fluorescence emission. The optical features and appearance of vitiligo have been explained primarily on the basis of reduced epidermal pigmentation, which results in abnormal white patches on the skin. The objective of this study is to explore the fluorescence properties of vitiligo and its adjacent normal skin using fluorescence excitation-emission matrix (EEM) spectroscopy. Thirty five (35) volunteers with vitiligo were acquired using a double-grating spectrofluorometer with excitation and emission wavelengths of 260-450 nm and 300-700 nm respectively. As expected, the most pronounced difference between the spectra obtained from vitiligo lesions compared to normally pigmented skin was that the overall fluorescence was much higher in vitiligo; these differences increased at shorter wavelengths, thus matching the characteristic spectral absorption of epidermal melanin. When comparing the fluorescence spectra from vitiligo to normal skin we detected three distinct spectral bands centered at 280nm, 310nm, and 335nm. The 280nm band may possibly be related to inflammation, whereas the 335 nm band may arise from collagen or keratin cross links. The source of the 310 nm band is uncertain; it is interesting to note its proximity to the 311 nm UV lamps used for vitiligo phototherapy. These differences are accounted for not only by changes in epidermal pigment content, but also by other optically active cutaneous biomolecules.

  12. MHC class II super-enhancer increases surface expression of HLA-DR and HLA-DQ and affects cytokine production in autoimmune vitiligo.

    Science.gov (United States)

    Cavalli, Giulio; Hayashi, Masahiro; Jin, Ying; Yorgov, Daniel; Santorico, Stephanie A; Holcomb, Cherie; Rastrou, Melinda; Erlich, Henry; Tengesdal, Isak W; Dagna, Lorenzo; Neff, C Preston; Palmer, Brent E; Spritz, Richard A; Dinarello, Charles A

    2016-02-02

    Genetic risk for autoimmunity in HLA genes is most often attributed to structural specificity resulting in presentation of self-antigens. Autoimmune vitiligo is strongly associated with the MHC class II region. Here, we fine-map vitiligo MHC class II genetic risk to three SNPs only 47 bp apart, located within a predicted super-enhancer in an intergenic region between HLA-DRB1 and HLA-DQA1, localized by a genome-wide association study of 2,853 Caucasian vitiligo patients. The super-enhancer corresponds to an expression quantitative trait locus for expression of HLA-DR and HLA-DQ RNA; we observed elevated surface expression of HLA-DR (P = 0.008) and HLA-DQ (P = 0.02) on monocytes from healthy subjects homozygous for the high-risk SNP haplotype. Unexpectedly, pathogen-stimulated peripheral blood mononuclear cells from subjects homozygous for the high-risk super-enhancer haplotype exhibited greater increase in production of IFN-γ and IL-1β than cells from subjects homozygous for the low-risk haplotype. Specifically, production of IFN-γ on stimulation of dectin-1, mannose, and Toll-like receptors with Candida albicans and Staphylococcus epidermidis was 2.5- and 2.9-fold higher in high-risk subjects than in low-risk subjects, respectively (P = 0.007 and P = 0.01). Similarly, production of IL-1β was fivefold higher in high-risk subjects than in low-risk subjects (P = 0.02). Increased production of immunostimulatory cytokines in subjects carrying the high-risk haplotype may act as an "adjuvant" during the presentation of autoantigens, tying together genetic variation in the MHC with the development of autoimmunity. This study demonstrates that for risk of autoimmune vitiligo, expression level of HLA class II molecules is as or more important than antigen specificity.

  13. Clinical application of phototherapy in the treatment of vitiligo%光疗在白癜风治疗中的临床应用

    Institute of Scientific and Technical Information of China (English)

    龚洋洋; 陈崑

    2016-01-01

    白癜风是一种常见的慢性获得性色素减少性疾病,其发病机制尚不清楚,可能的机制有自身免疫、黑素细胞自毁、神经精神因素、表皮因子失衡及遗传因素等.临床上治疗白癜风的方法很多,光疗及其联合疗法是目前有效的治疗方法之一,尤其适用于泛发性及难治性白癜风.而临床应用中,光疗疗效受多因素影响,如疾病分型、患病年龄、皮损部位等.因此了解各种光疗的作用机制,适应证,临床疗效及不良反应,有助于临床医生选择最佳的治疗方案.%Vitiligo is a kind of common chronic acquired hypopigmented skin disease with unclear pathogenesis,which may involve autoimmunity,self-destruction of melanocytes,neural or psychic factors,imbalance of epidermal factors,genetic factors,and so on.There are many treatments of vitiligo in clinic,and phototherapy alone or in combination with other therapy is effective,especially for generalized and refractory vitiligo.In practice,the efficacy of phototherapy is associated with various factors,such as types of vitiligo,sites of lesions,patients' age,etc.Thus,to understand action mechanisms,indications,therapeutic effects and adverse effects of phototherapy may be helpful in selecting the best treatment protocol.

  14. Gingival Vitiligo: Report of a Case and Review of the Literature

    Directory of Open Access Journals (Sweden)

    Nipun Ashok

    2014-01-01

    Full Text Available Rarely cases have been reported regarding depigmented lesions of the oral cavity. On reviewing the literature, only few cases of gingival vitiligo or similar lesions have been reported till date. These lesions pose a cosmetic challenge. We present here a case of vitiligo affecting gingiva. Vitiligo has been defined as an acquired, slowly progressive loss of cutaneous pigment which occurs as irregular, sharply defined patches which may or may not be surrounded by macroscopic hyperpigmentation. Differential diagnosis, detailed clinical history, histopathology, immunohistochemistry, and pathogenesis of this condition are discussed.

  15. Evolution and evaluation of autologous mini punch grafting in vitiligo

    Directory of Open Access Journals (Sweden)

    Lahiri Koushik

    2009-01-01

    Full Text Available Vitiligo is a result of disrupted epidermal melanization with an undecided etiology and incompletely understood pathogenesis. Various treatment options have resulted in various degrees of success. Various surgical modalities and transplantation techniques have evolved during the last few decades. Of them, miniature punch grafting (PG has established its place as the easiest, fastest, and least expensive method. Various aspects of this particular procedure have been discussed here. The historical perspective, the instruments, evolution of mini grafting down the ages, and the methodology, advantages, and disadvantages have been discussed. A detailed discussion on the topic along with a review of relevant literature has been provided in this article.

  16. Therapeutic spot and regional dermabrasion in stable vitiligo

    Directory of Open Access Journals (Sweden)

    Savant S

    1996-01-01

    Full Text Available Therapeutic spot or regional dermabrasion was carried out at 64 sites located over cosmetically unimportant hairy areas (52 and non-hairy areas (12 in 15 cases of stable vitiligo. Lesions were individually dermabraded first with either electric or manual dermabraders till pinpoint bleeding occurred. They were further deep demabraded to an appropriate depth manually. On healing, all the 64 sites were further treated with PUVA or PUVASOL. Out of 52 hairy sites 46 sites (88.5% showed total pigmentation, 2 showed partial pigmentation and in 4 sites there was no pigmentation. Out of 12 non-hairy sites, 10 sites (88.3% showed only perilesional hyperpigmentation along the borders with no change in the remaining 2 sites. Side effects were superficial scarring (5 and hypopigmentation (4 which improved over next 6 months. Complications were deep scarring (3 and secondary infection (1. Therapeutic spot or regional dermabrasion is useful alone or in combination with PUVA/PUVASOL for treating stable vitiligo (hairy areas.

  17. Functionally stable plasminogen activator inhibitor-1 in a family with cardiovascular disease and vitiligo.

    Science.gov (United States)

    Agirbasli, Mehmet; Eren, Mesut; Yasar, Songul; Delil, Kenan; Goktay, Fatih; Oner, Ebru Toksoy; Vaughan, Douglas E

    2014-07-01

    Vitiligo is a common skin condition with a complex pathophysiology characterized by the lack of pigmentation due to melanocyte degeneration. In this study, we investigated PAI-1 antigen (Ag) and activity levels in a 34 year old male with extensive vascular disease, alopecia areata and vitiligo. Fasting PAI-1 Ag and activity levels were measured at 9 a.m. in the subject and family members. Both PAI-1 Ag (67 ± 38 vs. 18.6 ± 6.5 ng/ml, P vitiligo is not known, it is likely due to post-translational modifications or increased binding affinity for a stabilizing cofactor. In conclusion, enhanced stability of PAI-1 may contribute to the pathophysiology of vascular disease and associated melanocyte degeneration. Systemic or local treatment with PAI-1 inhibitors may offer a potential treatment alternative to the near orphan status for vitiligo drug development.

  18. Rapid Partial Repigmentation of Vitiligo in a Young Female Adult with a Gluten-Free Diet

    OpenAIRE

    Birgit N. Khandalavala; Nirmalraj, Maya C.

    2014-01-01

    Vitiligo is a common pigmentary skin condition with a profound impact. Despite a number of therapeutic modalities, few have been demonstrated to result in significant repigmentation within a brief period of time. Reported dietary interventions are sparse. Following exclusion of gluten in the diet, early and extensive repigmentation of facial lesions were noted in a young female adult of Asian ethnicity with acrofacial vitiligo. The majority of the benefits occurred within the first month and ...

  19. A novel scoring system for evaluation of results of autologous transplantation methods in vitiligo

    Directory of Open Access Journals (Sweden)

    Gupta Somesh

    2002-01-01

    Full Text Available Autologous transplantation of melanocyte/melanocytes bearing epidermis for vitiligo can be done by mini-punch grafting (MPG, suction blister epidermal grafting (SBEG, thin split -thickness skin grafting (TSTG, transplantation of basal cell layer enriched suspension and cultured epithelial/melanocyte transplantation. To date no comparative study of these transplantation procedures has been published. Comparison of different studies carried out at different centers may be difficult in the absence of uniform evaluation criteria. In most of the published studies, the results were evaluated in terms of extent of pigmentation. Complictions and color match were evaluated separately. This approach, however, may not give a fair idea about the results. We have developed a scoring system with holistic approach considering the extent of pigmentation, color match and the complications of both the donor and the recipient areas, all taken together. In the scoring system, the score for individual criteria was multiplied with a factor, the value of which was decided on the basis of relative importance of each criteria. The use of this scoring system is exemplified in twelve patients who underwent TSTG, SBEG and MPG. In the scoring system the results were judged as excellent and fair in 3 patients each, as good in 4 patients and as poor in 2 patients.

  20. 儿童节段型白癜风的临床特点分析%Clinical analysis of segmental vitiligo in children

    Institute of Scientific and Technical Information of China (English)

    李阳; 祝逸萍; 许爱娥

    2013-01-01

    Objective To analyze the clinical features and evolution ot segmental vitiligo in children.Methods A standardized questionnaire was used to clinically investigate segmental vitiligo in 170 children aged < or =12 years and 174 adults with an age of onset > 18 years.Data analysis was done by the software SPSS16.0.Results The female to male ratio was 108:62 in the 170 child patients,significantly different from that in the adult patients (x2 =14.44,P < 0.05).Localized vitiligo occurred as the initial presentation in 82.9% (141/170) ofthe children and 76.4% (133/174) of the adults,and progressed into segmental vitiligo in half a year in 76.5% of the 141 children and 63.9% of the 133 adults.Vitiligo entered the quiescent stage after half-a-year progression in 71.6% of the children and 67.3% of the adults with localized vitiligo as the initial presentation,and after one-month progression in 62.1% of the children and 41.5% of the adults with segmental vitiligo as the first presentation (x2 =8.39,P < 0.01).Head and face were affected at the onset of segmental vitiligo in 44.1% of the childhood cases and 56.9% of the adult cases.Single nerve segments were involved at the onset in 94.7% of the child patients and 86.8% of the adult patients (x2 =0.04,P > 0.05).The proportions of patients with white hairs,autoimmune diseases,and family history were significantly lower in the child patients than in the adult patients (x2 =15.88,5.62,6.66 respectively,all P < 0.05).Conclusions Childhood-onset segmental vitiligo shows a predilection for males,usually presents as localized vitiligo at the onset,and becomes quiescent after half-a-year progression in more than 70% of patients.%目的 了解儿童节段型白癜风的临床特点及其病情变化.方法 采用问卷调查方法收集2011年10月至2012年7月于我科门诊就诊的170例儿童节段型白癜风患者的临床流行病学资料,对照组为发病年龄在18岁以上的174例

  1. Relationship between vitiligo and thyroid diseases%白癜风与甲状腺疾病的相关性研究

    Institute of Scientific and Technical Information of China (English)

    蒋跃根; 王维; 王峰来; 蔡秀玲; 程险峰

    2015-01-01

    目的:研究白癜风与甲状腺疾病的相关性。方法采用化学发光法检测289例白癜风患者和128例健康者血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)、抗甲状腺过氧化物酶抗体(TPO‐Ab)、抗甲状腺球蛋白抗体(TG‐Ab)水平。对检测结果进行统计学分析。结果白癜风患者FT3、TG‐Ab、TPO‐Ab异常率高于健康者(P<0.05)。白癜风合并甲状腺疾病发病率与白癜风患者年龄、性别有关(P<0.05)。结论白癜风患者存在不同程度的甲状腺功能指标及自身抗体水平的异常,男性和青少年白癜风患者更为明显。因此有必要对白癜风患者进行甲状腺疾病指标检测。%Objective To investigate the relationship between vitiligo and thyroid diseases .Methods Chemiluminescence was used to detect serum levels of free triiodothyronine (FT3) ,free thyroxine(FT4) ,thyrotropic stimulating hormone(TSH) ,anti‐thy‐roglobulin antibody(TG‐Ab) and anti‐thyroperoxidase antibody(TPO‐Ab) in 289 vitiligo patients and 128 healthy subjects .All re‐sults were statistically analyzed .Results The abnormal rates of FT3 ,TG‐Ab and TPO‐Ab in vitiligo patients were higher than healthy subjects (P<0 .05) ,which might be correlated with the age and gender of vilitigo patients .Conclusion Levels of thyroid function and autoantibody might be abnormal in vilitigo patients ,which could be more obvious in male and adolescent patients .It could be advantageous to screen thyroid function and antibody levels in patients with vitiligo .

  2. A comparative study of mitochondrial ultrastructure in melanocytes from perilesional vitiligo skin and perilesional halo nevi skin.

    Science.gov (United States)

    Ding, Gao-Zhong; Zhao, Wen-E; Li, Xue; Gong, Qing-Li; Lu, Yan

    2015-04-01

    Vitiligo and halo nevi are both pigmentary disorders of the skin characterized by the acquired loss of functional epidermal melanocytes manifesting as white macules and patches. The cellular mechanism(s) and biochemical changes that result in the appearance of these two types of achromic lesions are still uncertain; and the relationship between vitiligo and halo nevi has been in dispute. In this study, we investigated the ultrastructure of mitochondria in melanocytes and in keratinocytes from perilesional vitiligo skin and from perilesional halo nevi skin using Transmission Electron Microscopy. Furthermore, we performed a quantitative analysis of mitochondrial morphology through a stereological study. As previously reported, we found that melanocytes from perilesional active vitiligo skin were loosely connected with their surroundings by their retracted dendrites. The surface density and the volume density of mitochondria in melanocytes and in keratinocytes from perilesional vitiligo skin are increased significantly compared with the controls, especially in active vitiligo. In contrast, there are no significant differences in mitochondria in melanocytes and in keratinocytes from perilesional halo nevi skin compared with the controls. In summary, the tendency of different morphologic alterations in mitochondria from perilesional vitiligo skin and from perilesional halo nevi skin reflect heterogeneous backgrounds between the two diseases, revealing that vitiligo and halo nevi may have separate pathogenic mechanisms. These findings may help elucidate the relationship of these two diseases and their underlying mechanisms.

  3. Why treatments do(n't) work in vitiligo: An autoinflammatory perspective.

    Science.gov (United States)

    Speeckaert, Reinhart; Speeckaert, Marijn M; van Geel, Nanja

    2015-04-01

    Vitiligo is a recalcitrant depigmentary skin disorder with significant effects on the quality of life and a frequent association with other autoimmune disorders. The results of the current therapeutic options remain variable and treatment resistance is often encountered. The mainstay of treatment remains topical corticosteroids, topical calcineurin inhibitors and UVB therapy. In more extensive or progressive cases, systemic corticosteroids are effective although their prolonged use is hampered due to safety concerns. A lot of topical and systemic treatments have been investigated during the last decades. Given the elevated TNF-α levels in vitiligo lesions, the failure and even paradoxal effects of TNF-α inhibitors were highly remarkable. Nonetheless, a lot of progress has been made to unravel the pathophysiology of vitiligo. In this review, we provide an overview of the currently known underlying mechanisms leading to vitiligo and link this to the success or failure of treatments that have been used in clinical trials. We believe that this overview can direct future vitiligo research and rationalise the treatment options.

  4. Updata of trichrome vitiligo%三色白癜风研究进展

    Institute of Scientific and Technical Information of China (English)

    柳君如; 刘卫兵; 张峻岭

    2015-01-01

    Trichrome vitiligo is a special type of vitiligo. Trichrome vitiligo consists of an intermediate zone of hypopigmentation located between the depigmented center and the normal unaffected skin. The onset of tri-chrome vitiligo is associated with immunity, nerve chemical factors and gene. In this paper, we reviewed the pathogenesis, clinical and pathological characteristics, and treatment of trichrome vitiligo.%三色白癜风是寻常型白癜风临床表现之一,指正常肤色与色素完全脱失斑之间有宽窄不一的中间带,中间带的颜色淡于正常肤色而深于色素完全脱失斑,其发病往往与自身免疫、神经化学因子、遗传有关。本文对三色白癜风的发病机制、临床病理特征及治疗进展作一综述。

  5. Vitiligo and systemic disorders%白癜风与系统性疾病

    Institute of Scientific and Technical Information of China (English)

    吴辛刚; 许爱娥; 宋秀祖

    2009-01-01

    Vitiligo is a common skin disease characterized by depigmented macules.It is closely associated with the pathogenesis of several systemic disorders.In addition,vitiligo-like depigmentary patches may be concurrent with the development of some systemic diseases,which makes it difficult to difTerentiate from vitiligo.This article presents the relationship between vitiligo and some systemic disordcrs.which will contribute to a better diagnosis and treatment of vitiligo.%白癜风是一种常见的、以色素脱失斑为主要表现的皮肤病.临床上一些系统性疾病的发病过程中出现白癜风或其发病与白癜风密切相关.此外,一些系统性疾病的发病过程中表现为类似白癜风样的色素脱失斑,与白癜风鉴别较为困难.为此介绍这些系统性疾病与白癜风的相关性,以便做出正确的诊断和治疗.

  6. Psoriasis lineal y vitiligo segmentario, manifestación sobrepuesta de dos enfermedades frecuentes Lineal psoriasis and segmental vitiligo overlapped. Presentation of two frequent conditions

    Directory of Open Access Journals (Sweden)

    MC Valbuena

    Full Text Available La asociación de psoriasis y vitiligo es un evento bien documentado, con una incidencia del 3%, según diferentes series de casos, aunque no hallamos trabajos de superposición de ambas enfermedades siguiendo las líneas de Blaschko, en la literatura revisada por nosotros. Presentamos el caso de una mujer de 47 años de edad con lesiones de vitiligo en rostro y en abdomen, desde la infancia, que se distribuyeron sobre las líneas de Blaschko; después de 30 años aparecieron placas eritematoescamosas sobre las manchas acrómicas del abdomen, que clínica e histopatológicamente correspondieron a psoriasis y mejoraron parcialmente con fototerapia UVB de banda estrecha. La psoriasis y el vitiligo son entidades multifactoriales, poligénicas, que pueden exhibir patrones de mosaicismo cutáneo. Se han planteado algunas teorías para explicar este fenómeno, pero todavía no son conocidos todos los factores que influyen en este tipo de presentación.The association of psoriasis and vitiligo is a well documented event, with an incidence of 3% according to different case series, but reports of an overlap of both diseases following Blaschko lines were not found in the reviewed literature. We present the case of a 47 years old woman with vitiligo lesions in the face and abdomen, starting since childhood, distributed over the Blaschko lines; thirty years later erythematous and squamous plaques appeared over the achromic macules of the abdominal region which were clinically and histopathologically compatible with psoriasis and improved partially with narrow band UVB phototherapy. Psoriasis and vitíligo are multifactorial and polygenic skin disorders that can show patterns of cutaneous mosaicism. Some theories have tried to explain this phenomenon, but the factors that influence this presentation are still unclear.

  7. Clinical features of 378 cases of childhood vitiligo%儿童白癜风378例临床分析

    Institute of Scientific and Technical Information of China (English)

    蒋小音; 袁江; 沈斌; 王文娟

    2011-01-01

    Objective The clinical data of 378 childhood vitiligo patients were analyzed in this study in order to understand the clinical characteris tics of vitiligo in children. Methods Questionnaires have been used to collect the clinical data on 378 cases childhood vitiligo patient for statistical analysis. Results Of the 378 children 183 (48.2%) were boys and 195 (51.8%) were girls. The mean age of the disease was (9.56±2.21) years. The mean age of onset of the disease was (7.69± 2.31) years. The most common clinical type was localized type; the common season of onset was summer; 43 cases (11.4%) had family history, 262 patients (69.3%) are in advanced stage, 18 cases (4.8%) with halo nevus, 56 cases of partial eclipse (14.8%), history of trauma in 22 cases (5.8%), psychological factors 16 cases (4.2%) were the main triggers of children with vitiligo, There were significantly different in the incidence of rural and urban children (P<0.05), but combined autoimmune diseases were rare. Conclusion Childhood vitiligo may is distinct type of vitiligo.%目的:通过分析378例儿童白癜风的临床资料,了解儿童白癜风的临床特点.方法:采取调查问卷方式收集378例儿童白癜风门诊患者临床资料并进行整理、统计分析.结果:儿童患者共378例,其中男183例(48.2%),女195例(51.8%),平均年龄为(9.56±2.21)岁,平均病程为(20.0±22.08)个月,平均发病年龄为(7.69±2.31)岁,局限性白癜风多见,好发季节为夏季,43例(11.4%)有家族史,262例(69.3%)处于进展期,18例(4.8%)伴有晕痣,偏食56例(14.8%)、外伤史22例(5.8%)、精神因素16例(4.2%)是儿童白癜风主要的诱发因素,城乡患儿发病率有显著性差异(P<0.05),伴发自身免疫性疾病少见.结论:儿童型白癜风可能是一种特殊类型的白癜风.

  8. Expression of the CLEC2B mRNA and Protein in Vitiligo%白癜风患者皮损CLEC2B mRNA及其蛋白表达

    Institute of Scientific and Technical Information of China (English)

    于旺; 张峻岭; 马秀亮; 索丹凤; 闫翠彦

    2013-01-01

    Objective To investigate the CLEC2B mRNA expression level and the protein location in the lesion of vitiligo,and to define the cellular localization of CLEC2B.Methods Use real-time fluorescence quantitative reverse transcription-polymerase chain reaction method (RT-PCR) to detect the CLEC2B mRNA expression levels of vitiligo patients and normal control group lesion,take the higher transcriptional expression level of CLEC2B as the object,to make sure the positioning of the protein of CLEC2B in vitiligo lesion using immunohistochemical methods.Results The expression of CLEC2B mRNA in vitiligo lesions was significantly higher than that of normal control (t=4.432,P<0.05).The CLEC2B protein was expressed in the dermal layer of lymphocyte nucleus of vitiligo lesions.Conclusion The CLEC2B is highly expressed in the lesion of vitiligo,in the exact,and also in the dermal layer of lymphocyte nucleus.%目的 探讨白癜风患者皮损区CLEC2B(C-type lectin domain family 2,member B)mRNA表达水平及其蛋白的表达,明确CLEC2B基因表达的细胞定位.方法 应用实时荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测白癜风患者与正常对照组皮损区中CLEC2B mRNA表达量,取CLEC2B转录表达明显增高的白癜风患者皮损组织,用免疫组织化学方法进行皮损区CLEC2B蛋白的定位表达.结果 患者组皮损中CLEC2B mRNA表达量高于正常对照组(t=4.432,P<0.05),在白癜风皮肤组织真皮层的淋巴细胞中有CLEC2B蛋白阳性表达.结论 CLEC2B基因在白癜风皮损中高表达,且在真皮层淋巴细胞的细胞核中也有表达.

  9. 唯尔本治疗白癜风及其对患者免疫功能的影响%Effect of immunological function of vitiligo treated with Weierben

    Institute of Scientific and Technical Information of China (English)

    孙祥银; 缪泽群; 陈宏翔

    2001-01-01

    We examined lymphocyte transformation efficiency, IgG, IgA and IgM of 46 cases of vitiligo. The patients were treated with Weierben injection. The immunologic indexes were compared in 20 cases randomly in antero-and posterio-treatment(P<0.05). The results showed that the patients with vitiligo had immunologic abnormity apparently, especially in evolution period. The abnormity had nothing to do with the period of illness and types of the disease. After treated with Weierben, the patients with vitiligo vulgaris were cured in 13.15% and improved in 64.86%; and vitiligo segmentum cases were improved in 77.78%.%检测了46例患者的淋巴细胞转化率,IgG,IgA,IgM,应用唯尔本进行了治疗。结果表明:白癜风患者确有免疫功能异常,进展期尤为明显,而免疫功能的异常与患者的病期及临床表现类型无明显的关系;唯尔本对寻常型患者治愈率为13.15%,总有效率为64.86%;对节段型总有效率也达77.78%。20例患者治疗前后细胞免疫及体液免疫指标具有显著性差异。

  10. 白癜风患者CLEC2B mRNA差异表达与临床相关性研究%Differential expression of CLEC2B mRNA in vitiligo and its clinical implications

    Institute of Scientific and Technical Information of China (English)

    张峻岭; 于旺; Youwen Zhou; 马秀亮; 索丹凤; 闫翠彦

    2013-01-01

    Objective To evaluate the role of C-type lectin domain family 2,member B (CLEC2B) gene in the pathogenesis of vitiligo.Methods Real time fluorescence-based PCR was performed to detect the expression of CLEC2B mRNA in the peripheral blood and lesional skin of 37 patients with vitiligo as well as in the peripheral blood and normal skin of 40 healthy controls.Data were statistically analyzed by t test and chisquare test.Results Among the 37 patients,23 had progressive vitiligo,14 stable vitiligo,31 vitiligo vulgaris,6 segmental vitiligo.The expression level of CLEC2B mRNA was significantly higher in vitiligo lesions than in the control skin (1.21 ± 0.03 vs.1.00,t =4.432,P < 0.05),but was of no significant difference in peripheral blood between the patients and healthy controls (1.02 ± 0.05 vs.1.00,t =1.435,P > 0.05).Increased expression of CLEC2B mRNA was noted in lesions of vulgaris vitiligo compared with those of segmental vitiligo (1.21 ± 0.03 vs.1.02 ± 0.01,t =5.330,P < 0.05),as well as in lesions of progressive vitiligo compared with those of stable vitiligo (1.25 ± 0.05 vs.1.08 ± 0.03,t =3.046,P < 0.05).No significant difference was observed in the expression of CLEC2B mRNA among lesions of vitiligo with different courses (P > 0.05).Conclusion The differential expression of CLEC2B mRNA may take part in the pathogenesis of vitiligo.%目的 探讨CLEC2B(C-type lectin domain family 2,member B)基因在白癜风发病中的作用.方法 实时荧光定量PCR方法检测白癜风患者皮损区及外周血中CLEC2B mRNA表达,比较不同临床类型、病期、病程CLEC2B mRNA的差异表达.结果 白癜风患者组皮损中,CLEC2B mRNA表达量高于健康对照组(两组表达量分别为1.21±0.03,1.00),差异有统计学意义(t=4.432,P<0.05).白癜风患者与健康对照组外周血CLEC2B mRNA表达量比较,差异无统计学意义(两组表达量分别为1.02±0.05,1.00;t=1.435,P> 0.05).寻常型白癜风皮损组中,CLEC2B mRNA表达

  11. A Comparative Study Of Psychiatric Morbidity In Dermatological Patients

    Directory of Open Access Journals (Sweden)

    Sharma Neelu

    2003-01-01

    Full Text Available The psychiatric morbidity in five chronic and disfiguring diseases, namely psoriasis, chronic urticaria, leprosy, vitiligo and lichen simplex chronicus (LSC was assessed and compared using the standardized Hindi (Vernacular languages version of General Health Questionnaire (GHQ-H. Thirty new untreated patients of each of the above skin diseases between the age group of 18-60 years were included in the study. The overall prevalence of the psychiatric morbidity was found to be 39%, depression and anxiety were present in 13% and 10.66% of the patients and suicidal ideations and somatisation in 16% and 13% of the patients respectively. Prevalence of interpersonal conflict and suicidal attempt were 10% and 2.6% respectively. On comparative analysis of psychiatric morbidity, significant difference was observed between vitiligo and other disorders (p=0.0028, i.e., chronic urticaria (p=0.0242 and psoriasis and other disorders (p=0.0028, however no significant difference could be elicited between psoriasis and leprosy or leprosy and vitiligo. Comparative analysis of anxiety revealed statistically significant difference between the patients of LSC and vitiligo (p=0.02 or vitiligo and chronic urticaria (p=0.04 but no significant difference was observed for vitiligo and leprosy of psoriasis and leprosy. The prevalence of somatic complaints showed significant difference between the patients of LSC and Leprosy.

  12. Efficacy of 308 nm excimer laser on vitiligo%308 nm 准分子激光治疗白癜风

    Institute of Scientific and Technical Information of China (English)

    王国学

    2015-01-01

    目的:探讨308 nm 准分子激光局部照射治疗白癜风的疗效。方法选择2010年8月至2012年10月收治的白癜风患者56例,给予308 nm 准分子激光局部照射,每周2次,随访6个月,观察其疗效。结果经过准分子光局部照射平均26.3次,76.5%皮损有不同程度色素恢复,色素恢复随疗程延长而增加,照射45次总有效率为92.6%。躯干、颈部、头部疗效优于四肢、手足,泛发型和节段型白癜风。主要不良反应为局部水疱和疼痛,患者能耐受。结论准分子光局部照射治疗白癜风疗效显著且不良反应少,其疗效与皮损解剖部位相关。%Objective To investigate the efficacy of 308 nm excimer laser on vitiligo. Methods From Aygyst 2010 to Octo-ber 2012,fifty six patients with vitiligo were selected and were given 308 nm excimer laser,twice a week and followed yp for 6 months,the effect was observed. Results After average 26. 3 times of local irradiation of excimer light,76. 5% had varying de-grees of repigmentation of lesions,pigmented recovered increases with prolonged treatment,irradiation of 45 times the total effi-ciency rate was 92. 6% . Torso,neck,head were better than the limbs,hands and feet,generalized and segmental vitiligo had a good effect. The main adverse reactions were local blisters and pain,and the patients coyld tolerate. Conclusion The effect of excimer laser on vitiligo is significantly and has fewer adverse reactions,and its efficacy is associated with lesions anatomy.

  13. The Clinical Analysis of Treatment Vitiligo with Intense Pulse Light%强脉冲光治疗白癜风的临床分析

    Institute of Scientific and Technical Information of China (English)

    黄伟

    2010-01-01

    Objective To evaluate the clinical efficacy of treatment vitiligo with 320 nm intense pulse light.Methods 126 cases of patients with vitiligo vulgaris were irradiated with 320 nm intense pulse light on personalized treatment parameters once a week,and the clinical efficacy was evaluated after 10 and 20 times treatment.Results After 10 and 20 times of treatment,the total effective rate of 126 patients with vitiligo was 77.0%(97/126)and 90.5 %(114/126)respectively.From the perspective of skin lesions,the effective rate in face-neck,trunk and extremities was 83.1%(49/59),79.6%(35/44)and 56.5%(13/23)respectively after 10 times treatment.The rate in face-neck and turnk was higher than in extremities(P < 0.05),but the rate in face-neck and in trunk was not obvious differences(P < 0.05).Conclusion Treatment vitiligo with 320 nm intense pulse light is effective,convenient and safe.%目的 观察应用320 nm强脉冲光治疗白癜风的临床效果.方法 126例寻常型白癜风患者,应用320 nm强脉冲光选择个性化治疗参数对皮损进行照射,1次/周,10次和20次治疗后评价其临床效果.结果 10次治疗结束后,总有效率为77.0%(97/126),20次治疗结束后,有效率为90.5%(114/126);从皮损部位看,面颈部、躯干部和四肢在10次治疗结束后的有效率,分别为83.1%(49/59)、79.6%(35/44)和56.5%(13/23),面颈部和躯干的有效率明显高于四肢(P<0.05),而颈部与躯干部的有效率无明显差异(P>0.05).结论 应用320 nm强脉冲光治疗白癜风的临床效果肯定,方便安全.

  14. Topical delivery of clobetasol propionate loaded microemulsion based gel for effective treatment of vitiligo--part II: rheological characterization and in vivo assessment through dermatopharmacokinetic and pilot clinical studies.

    Science.gov (United States)

    Patel, Hetal K; Barot, Bhavesh S; Parejiya, Punit B; Shelat, Pragna K; Shukla, Arunkumar

    2014-07-01

    Vitiligo is a non contagious acquired pigmentation disorder with limited treatment possibilities. Clobetasol propionate (CP) is the drug-of-choice for vitiligo which suppresses the immune system by reducing immunoglobulin action and causes the restoration of melanocytes leading to repigmentation of skin. However, despite being effective, its low and variable bioavailability prompt for development of novel carrier that could effectively target CP to site of action without producing undesirable side-effects. Low solubility of CP in subsequent poor in vivo bioavailability was overcome by formulating microemulsion based gel of CP (MBC) which would enhance the percutaneous transport of CP into and across the skin barrier. Comprehensive characterization of MBC was carried out for viscosity, gel strength and rheological behavior. In vitro studies revealed much higher drug release, skin penetration and enhanced skin accumulation as compared to control (Cream of CP). In vitro and in vivo occlusion studies demonstrated similar occlusiveness for MBC and control. MBC exhibited 3.16 times higher stratum corneum CP levels compared to control. Visualization of cutaneous uptake in vivo using laser scanning microscopy confirmed targeting of CP to epidermis and dermis. Dermatopharmacokinetic studies of MBC showed enhanced drug deposition of CP in skin layers. MBC was assessed for in vivo efficacy by single blind randomized pilot clinical study. The efficacy was assessed by vitiligo area scoring index (VASI) method. After completion of trial, repigmentation of vitiligo patches in patients were evaluated and scored. MBC was superior in terms of faster repigmentation and efficacy when compared with control (p valuevitiligo patients.

  15. Updata on transplantion of melanocytes in the treatment of vitiligo%黑素细胞移植治疗白癜风的研究进展

    Institute of Scientific and Technical Information of China (English)

    秦晓明; 柯国琳; 杨孔超

    2015-01-01

    Transplantion of melanocytes is one of the methods in the treatment of vitiligo at stable stage, including cultured melanocyte transplantation and epidermal cells suspension transplantation. Co-culturing of melanocytes with adipose-derived stem cells may be a potential substitute for co-culturing with keratino-cytes. The efficiency of autologous melanocytes transplantation in vitiligo patients may be predicted by perile-sional skin-homing CD8+T cell activities and the dermal mesenchymal stem cells might be used as auxiliary agent to improve the efficacy. The progress on transplantion of melanocytes in the treatment of vitiligo is re-viewed.%黑素细胞移植是治疗稳定期白癜风的方法之一,主要包括培养的黑素细胞移植和表皮细胞悬液移植。近年来动物实验证实脂肪干细胞可代替角质形成细胞与黑素细胞共培养移植治疗白癜风。白癜风患者自体黑素细胞的移植效率可通过皮损周围皮肤归巢CD8+T细胞活动性来进行预测,真皮间充质干细胞可作为黑素细胞移植治疗白癜风的辅助剂。本文综述黑素细胞移植治疗白癜风的国内外进展,旨在为临床提供参考。

  16. Use of tacrolimus ointment in vitiligo alone or in combination therapy.

    Science.gov (United States)

    Berti, S; Buggiani, G; Lotti, T

    2009-05-01

    Current treatments for vitiligo are largely unsatisfactory. Topical corticosteroids and phototherapy (narrow-band UVB and psoralen+UVA) are the most prescribed, however, these therapies are often not effective and have important side-effect, especially when used for a long time. Many studies have reported the efficacy and safety of tacrolimus ointment in adults and children with vitiligo, particularly when located on the head and neck. Successful treatment is possible when it is combined with other therapies, such as narrow-band UVB, microphototherapy, helium-neon laser, or narrow-band excimer laser.

  17. A Comparative Study on Levels of Oxidant-antioxidant in the Tissue Fluid from Skin of Vitiligo%白癜风患者皮肤组织液氧化-抗氧化物水平比较分析

    Institute of Scientific and Technical Information of China (English)

    李伟; 李世远; 陆健群; 黄耿; 赵进

    2012-01-01

    目的 探讨体内氧化-抗氧化状态与白癜风发病的关系.方法 采用化学比色法,对24例白癜风患者白斑和非白斑(正常部位皮肤)以及10例健康者组织液进行过氧化氢(H2O2)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)检测.结果 白癜风患者白斑H2O2水平(53.97±19.03)mmol/L明显高于健康组(28.98±22.81) mmol/L,进展期患者白斑H2O2水平(56.64±19.91) mmol/L明显高于非白斑(34.71±22.23)mmol/L,差异均有统计学意义(P均<0.01);白癜风白斑CAT水平(17.34±11.05)U/mL明显低于健康组(41.29±16.57)U/mL,差异有统计学意义(P<0.01),进展期白斑CAT水平(13.63±8.32)U/mL低于非白斑(35.72±16.14)U/mL,差异有统计学意义(P<0.05);进展期患者的白斑与非白斑处GSH-PX均高于健康组,差异有统计学意义(P均< 0.05).结论 白癜风发病可能与H2O2增高、CAT降低等氧化-抗氧化失衡有关.%Objective To explore the role of the oxidant-antioxidant status in the pathogenesis of vitiligo. Methods The hydrogen peroxide (H2O2) ,catalase (CAT) and glutathione peroxide (GSH-PX) in suction blister fluid from involved skin and uninvolved skin of 24 vitiligo patients and 10 healthy controls were detected. Results The H2O2 levels which was(53.97±19.03)mmol/L in suction blister of involved skin of vitiligo was significantly higher than those in controls which was (28.98±22.81)mmol/L (P<0.01). Those levels which was (56.64±19.91)mmol/L in involved skin of active vitiligo was significantly higher than those in its uninvolved skin which was (34.71 ±22.23)mmol/L (P<0.01). The CAT activities which was (17.34±11.05) U/mL of involved skin in vitiligo was significantly lower than those in controls which was (41.29±16.57) U/mL (P<0.01). Those levels which was (13.63±8.32)U/mL in involved skin of active vitiligo was lower than those in its uninvolved skin which was(35.72±16.14)U/mL(P< 0.05). Both GSH-PX activities in involved skin and uninvolved

  18. The Newest Hypothesis about Vitiligo: Most of the Suggested Pathogeneses of Vitiligo Can Be Attributed to Lack of One Factor, Zinc-α2-Glycoprotein

    OpenAIRE

    Bagherani, Nooshin

    2012-01-01

    Zinc- α 2-glycoprotein (ZAG) is a recently identified adipokine, assigned to the chromosome 7q22.1. It is a multidisciplinary protein, which is secreted in various body fluids. The ZAG plays roles in lipolysis, regulation of metabolism, cell proliferation and differentiation, regulation of melanin synthesis, cell adhesion, immunoregulation, and so forth. Vitiligo is the most common depigmenting skin disorder, characterized by acquired, progressive, and circumscribed amelanosis of the skin and...

  19. Clinical efficacy of narrow-band UVB phototherapy combined with tacrolimus ointment in the treatment of vitiligo%窄谱中波紫外线联合他克莫司软膏治疗白癜风的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘树雷; 廖军; 邓晓玲; 王鹰; 孙仁美; 贺旭

    2014-01-01

    目的:探讨窄谱中波紫外线联合他克莫司软膏对白癜风的治疗作用。方法:收集我院2011年至2013年门诊共90例白癜风患者,随机分为两组,每组各45例患者,分为窄谱中波紫外线联合他克莫司软膏治疗组及他克莫司软膏治疗组。随访6个月,分析两组患者治愈率,并对不同部位、不同期、不同类型白癜风的治疗情况进行比较分析。结果:窄谱中波紫外线联合他克莫司软膏对白癜风的治愈率明显高于单用他克莫司软膏组,头面部白癜风治疗有效率优于肢端部、躯干四肢部,肢端部白癜风治疗效果最差。寻常型白癜风的疗效较节段型白癜风疗效好。进展期白癜风较稳定期疗效好。结论:窄谱中波紫外线联合他克莫司软膏对白癜风具有较好的治疗作用。%Objective: To investigate the therapeutic effect of narrow-band UVB combined with tacrolimus ointment for vitiligo treament. Methods:From 2011 to 2013, 90 cases of patients with vitiligo in our hospital , were randomly divided into two groups, each group 45 patients. They were divided into narrow-band UVB phototherapy combined with tacrolimus ointment treatment group and ta-crolimus ointment treatment group.Those patients were followed-up for 6 months.Two groups' cute rate were analyzed and different parts of the different phases, different types of vitiligo treatment were compared.Results: Narrow-band UVB phototherapy combined with tacrolimus ointment for vitiligo cure rate was significantly higher than that of single use of tacrolimus ointment group, the head and face of vitiligo treatment effective rate higher than the extremities, trunk limbs acral of vitiligo treatment effect is the worst.Treat-ment effect of vitiligo vulgaris is better than segmental vitiligo. Treatment effect of Vitiligo in progress period is better than it in table period. Conclusion:narrow-band UVB phototherapy combined with tacrolimus ointment

  20. Clinical characteristics and therapeutic efficacy analysis on 10000 cases of vitiligo%白癜风10000例临床特征及治疗效果分析

    Institute of Scientific and Technical Information of China (English)

    坚哲; 周福波; 栾琪; 马翠玲; 李春英; 高天文; 李凯; 刘邦民; 李强; 刘玲; 宋璞; 王筱雯; 李舒丽; 石琼

    2012-01-01

    Objective To evaluate clinical characteristics and therapeutic efficacy of vitiligo and probe into its pathogenesis. Methods Information on the clinical characteristics and therapeutic efficacy of patients with vitiligo were collected by a uniform questionnaire. Sex, onset age, duration of disease, occurrence site, lesion distribution, lesion stages, clinical type, lesion scope, iesion area, causes, complication, family history, occurrence season, the levels of cellular immunity and therapeutic efficacy were analyzed. Results 10000 patients with vitiligo were evaluated. The constitute rate of males to females was 1.14 : 1, and the average onset age was 19.98 years old. The results showed that 71% cases betide before age 25. Trunk and limbs were the most sites of onset. The most frequent occurrence season is summer. About 21% have definite causes. The case of vitiligo vulgaris was more than the segmental type and the number of sporadic type was the most type in vitiligo vulgaris. Patients with progressive vitiligo were more than the patients in stable period. 1294-cases had family history. The localized type presented better therapeutic efficacy. 77 cases were fully recovered and 554 cases were markedly improved after treatment for 6 months. The total efficiency was 38.12%. Conclusion Patients with vitiligo had younger onset age and the etiology is unclear. Some patients have a family history or cellular immune dysfunction. The therapeutic efficacy remains to be improved.%目的:总结与分析白癜风的临床特征和治疗效果,探讨白瘢风发病机理.方法:采用调查表的形式收集10000例白癜风患者的临床和治疗资料,从性别构成、发病年龄、病期、发病部位、皮损分布、皮损范围、皮损面积、诱发因素、疾病分期及分型、伴发疾病、家族史、发病或加重季节、细胞免疫水平、治疗效果等方面进行分析.结果:10000例白癜风患者男女性别构成约为1.14:1,平均发病年龄

  1. 免疫球蛋白和补体与白癜风发病的相关性研究%Correlation of Immunoglobulins and Complements with the Pathogenesis of Vitiligo

    Institute of Scientific and Technical Information of China (English)

    徐士福; 张峻岭; 马秀亮; 柳君如; 程琳

    2012-01-01

    Objective To investigate the correlation of serum immunoglobulins and complements with the pathogenesis of vitiligo. Methods The serum levels of IgG, IgA, IgM, C3, and C4 in 120 patients with vitiligo were determined by immu-noturbidimetry and then compared with those in 60 healthy cases. These 120 vitiligo patients were also divided into four groups according to their subtypes, and the serum levels of the above items were compared inter - groups. Results The serum C3 level in patients with vitiligo was significantly lower than that in the healthy controls ( P 0. 05 ). Conclusion The pathogenesis of vitiligo is associated with the humoral immunity to some extent.%目的 探讨免疫球蛋白和补体与白癜风发病的关系.方法 采用免疫散射比浊法检测120例白癜风患者血清免疫球蛋白IgG、IgA、IgM 和补体C3、C4水平,根据不同分型分为4组,并以60例正常人作为对照组进行比较.结果 白癜风组血清补体C3水平低于对照组,差异有统计学意义(P<0.01),IgG、IgA、IgM和C4水平与对照组比较,差异均无统计学意义(P>0.05).不同类型的白癜风患者血清IgG、IgA、IgM、C3和C4水平比较,差异均无统计学意义(P>0.05).结论 白癜风的发病与体液免疫有一定的关系.

  2. Recurrent post-herpetic erythema multiforme, herpes labialis and secondary vitiligo in siblings

    Directory of Open Access Journals (Sweden)

    Mittal R

    1996-01-01

    Full Text Available Presence of recurrent herpes labialis with resultant secondary vitiligo associated with recurrent erythema multiforme further leading to depigmentation of skin in relation to target shaped maculopapular lesions in two real sisters was observed as a unique phenomenon which probably has not been reported earlier.

  3. Occupational vitiligo due to unsuspected presence of phenolic antioxidant byproducts in commercial bulk rubber

    Energy Technology Data Exchange (ETDEWEB)

    O' Malley, M.A.; Mathias, C.G.; Priddy, M.; Molina, D.; Grote, A.A.; Halperin, W.E.

    1988-06-01

    We investigated the occurrence of cutaneous depigmentation (vitiligo) among employees of a company that manufactured hydraulic pumps. The interiors of these pumps were injection-molded with rubber. We identified a small but significant cluster of vitiligo cases among a group of employees who frequently handled the rubber used in this injection molding process. Although none of the additives specified in the rubber formulations was a phenolic or catecholic derivative, known to be potential causes of chemically induced vitiligo, gas chromatographic analysis identified a para-substituted phenol (2,4-di-tert-butylphenol, DTBP) in solid samples of the most frequently used rubber. Surface wipe analysis confirmed that workers could be exposed to DTBP from simple handling of the rubber. We subsequently established that the solid bulk rubber used as the base in these stock rubber formulations contained both DTBP and smaller quantities of p-tert-butylphenol. Both had formed as unsuspected byproducts during chemical synthesis of two antioxidants added to the solid bulk rubber by a major rubber supplier. We conclude that the unsuspected presence of potential chemical depigmenting agents in solid bulk rubber, from which industrial rubber products are formulated, may contribute to the occurrence of occupational vitiligo, and that a simple review of ingredients in rubber formulations is inadequate to detect their presence.

  4. 从血论治白癜风%Treatment of Vitiligo Based on Blood

    Institute of Scientific and Technical Information of China (English)

    陈艳萍; 王波

    2012-01-01

    Vitiligo is a common acquired skin depigmentation disease. Ancient physicians believed etiology and pathogenesis of vitiligo were exogenous pathogenic wind and blood problems. This paper analyzed and summarized that the main pathogenic factor is wind based on the naming of vitiligo. And according to the therapy of treating blood for wind diseases for activated blood making wind disappear, vitiligo should be treated based on treating blood.%白癜风是一种常见的后天性表皮色素脱失性皮肤病.古代医家对白癜风病因病机的认识多以风邪相搏、气血失和立论.文章试从白癜风的病名分析、归纳、总结出其主要致病因素为六淫之首的“风”邪,并将“治风先治血,血行风自灭”立为理论依据,以血论治白癜风.

  5. The effects of galangin on a mouse model of vitiligo induced by hydroquinone.

    Science.gov (United States)

    Huo, Shi-Xia; Liu, Xin-Ming; Ge, Chun-Hui; Gao, Li; Peng, Xiao-Ming; Zhao, Ping-Ping; Yan, Ming

    2014-10-01

    Galangin, the main active component of Alpinia officinarum Hance, was tested in a mouse model of vitiligo induced in C57BL/6 mice by the topical application of 2 mL of 2.5% hydroquinone daily to shaved areas (2 × 2 cm) of dorsal skin for 60 days. Thirty days after the final application of hydroquinone, galangin (0.425, and 4.25 mg/kg) was administered orally for 30 days. The hair colour darkened when it grew back after treatment, and histological analysis showed that the number of melanin-containing hair follicles had increased after treatment with all doses of galangin groups and 8-methoxypsoralen (8-MOP, the positive control) compared with the untreated vitiligo group (p vitiligo induced by hydroquinone in mice, with the activity related to concentrations of TYR, expression of TYR protein, activity of malondialdehyde and content of cholinesterase. Galangin may therefore be a potential candidate for the treatment of vitiligo, subject to further investigation.

  6. Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: formulation optimization, in vitro evaluation and preclinical assessment.

    Science.gov (United States)

    Garg, Bhawna Jain; Garg, Neeraj K; Beg, Sarwar; Singh, Bhupinder; Katare, Om Prakash

    2016-01-01

    The present investigation aimed for the development and characterization of ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery and effective treatment against vitiligo. The ethosomes were prepared by central composite design (CCD) and characterized for various quality attributes like vesicle shape, size, zeta potential, lamellarity, drug entrapment and drug leaching. The optimized ethosomes were subsequently incorporated int Carbopol® 934 gel and characterized for drug content, rheological behavior, texture profile, in vitro release, ex vivo skin permeation and retention, skin photosensitization and histopathological examination. Ethosomes were found to be spherical and multilamellar in structures having nanometric size range with narrow size distribution, and high encapsulation efficiency. Ethosomal formulations showed significant skin permeation and accumulation in the epidermal and dermal layers. The fluorescence microscopy study using 123 Rhodamine exhibited enhanced permeation of the drug-loaded ethosomes in the deeper layers of skin. Also, the developed formulation showed insignificant phototoxicity and erythema vis-à-vis the conventional cream. The results were cross-validated using histopathological examination of skin segments. In a nutshell, the ethosomes-based hydrogel formulation was found to be a promising drug delivery system demonstrating enhanced percutaneous penetration of methoxsalen with reduced phototoxicity and erythema, thus leading to improved patient compliance for the treatment against vitiligo.

  7. Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: formulation optimization, in vitro evaluation and preclinical assessment.

    Science.gov (United States)

    Garg, Bhawna Jain; Garg, Neeraj K; Beg, Sarwar; Singh, Bhupinder; Katare, Om Prakash

    2016-03-01

    The present investigation aimed for the development and characterization of ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery and effective treatment against vitiligo. The ethosomes were prepared by central composite design (CCD) and characterized for various quality attributes like vesicle shape, size, zeta potential, lamellarity, drug entrapment and drug leaching. The optimized ethosomes were subsequently incorporated int Carbopol® 934 gel and characterized for drug content, rheological behavior, texture profile, in vitro release, ex vivo skin permeation and retention, skin photosensitization and histopathological examination. Ethosomes were found to be spherical and multilamellar in structures having nanometric size range with narrow size distribution, and high encapsulation efficiency. Ethosomal formulations showed significant skin permeation and accumulation in the epidermal and dermal layers. The fluorescence microscopy study using 123 Rhodamine exhibited enhanced permeation of the drug-loaded ethosomes in the deeper layers of skin. Also, the developed formulation showed insignificant phototoxicity and erythema vis-à-vis the conventional cream. The results were cross-validated using histopathological examination of skin segments. In a nutshell, the ethosomes-based hydrogel formulation was found to be a promising drug delivery system demonstrating enhanced percutaneous penetration of methoxsalen with reduced phototoxicity and erythema, thus leading to improved patient compliance for the treatment against vitiligo.

  8. Role of recipient-site preparation techniques and post-operative wound dressing in the surgical management of vitiligo

    Directory of Open Access Journals (Sweden)

    Nour Al-Hadidi

    2015-01-01

    Full Text Available Vitiligo is an acquired skin disorder characterized by the destruction of melanocytes resulting in achromic macules and patches involving the affected skin. Multiple methods of treatments have emerged to manage vitiligo, including medical and surgical techniques. Among the surgical techniques described in the management of vitiligo are minipunch grafting, split-thickness skin grafting, hair follicle transplantation, suction blister grafting, and cultured and non-cultured autologous melanocyte transplantation. However, prior to grafting optimal recipient-site preparation is needed for graft survival and successful repigmentation outcomes. Similarly, post-operative care of the recipient site is vital to yielding a viable graft irrespective of the transplantation technique employed. This article reviews the multiple methods employed to prepare the recipient site in vitiligo surgeries and the post-surgical conditions which optimize graft viability. A pubmed search was conducted utilizing the key words listed below.

  9. Vitiligo em crianças: uma revisão de classificação, hipóteses sobre patogênese e tratamento Vitiligo in children: a review of classification, hypotheses of pathogenesis and treatment

    Directory of Open Access Journals (Sweden)

    Jane S. Bellet

    2005-12-01

    Full Text Available Vitiligo é um achado dermatológico relativamente comum, observado desde a Antiguidade. A doença caracteriza-se por despigmentação da pele, com perda de melanócitos ao exame histológico. Diversos fenótipos clínicos resultam em diferentes graus de morbidade. A causa do vitiligo ainda é desconhecida e a etiologia mais provável parece ser auto-imune. O tratamento é difícil e várias alternativas mostram um potencial terapêutico significativo. Nesta revisão, abordaremos a classificação do vitiligo na infância, as hipóteses sobre a patogênese e o tratamento.Vitiligo is a relatively common dermatologic finding and one that has been observed since ancient times. Depigmentation of the skin, with loss of melanocytes on histology characterizes this disorder. A range of clinical phenotypes lead to varying degrees of morbidity. The cause of vitiligo remains unknown, although an autoimmune pathogenesis seems most likely. Treatment also remains difficult. A number of new therapies show significant potential. In this review, we will focus on the classification of childhood vitiligo, hypotheses of pathogenesis and treatment.

  10. A Novel Linkage to Generalized Vitiligo on 4q13-q21 Identified in a Genomewide Linkage Analysis of Chinese Families

    OpenAIRE

    Chen, Jian-Jun; Huang, Wei; Gui, Jin-Ping; Yang, Sen; Zhou, Fu-Sheng; Xiong, Quan-Geng; Wu, Hong-Bo; Cui, Yong; Gao, Min; Li, Wei; Li, Jin-Xian; Yan, Kai-Lin; Yuan, Wen-tao; Xu, Shi-Jie; Liu, Jian-Jun

    2005-01-01

    Generalized vitiligo is a common, autoimmune, familial-clustering depigmentary disorder of the skin and hair that results from selective destruction of melanocytes. Generalized vitiligo is likely a heterogeneous disease, with five susceptibility loci reported so far—on chromosomes 1p31, 6p21, 7q, 8p, and 17p13—in white populations. To investigate vitiligo susceptibility loci in the Chinese population, we performed a genomewide linkage analysis in 57 multiplex Chinese families, each with at le...

  11. Predisposing genes for vitiligo%白癜风易感基因的研究进展

    Institute of Scientific and Technical Information of China (English)

    彭吕娇; 康晓静

    2014-01-01

    白癜风是一种获得性色素脱失性皮肤疾病,其发病机制复杂.近年来研究表明,白癜风是一种多基因遗传病,尤其是泛发性白癜风与遗传密切相关.在近年研究中,认为谷胱甘肽巯基转移酶基因、过氧化氢酶基因、Toll样受体家族基因、酪氨酸酶基因、盘状结构域受体基因、人类白细胞抗原等位基因和超氧化物歧化酶基因等是白癜风易感基因.探讨白癜风的易感基因为进一步阐明其发病机制提供依据,对白癜风尤其是泛发性白癜风的早期诊断、治疗、预防具有重要作用.%Vitiligo is an acquired dermatosis characterized principally by skin depigmentation with complex pathogenesis.Recent studies have indicated that vitiligo is a polygenic disease,and generalized vitiligo is particularly closely related to heredity.Many predisposing genes have been discovered for vitiligo,including the glutathione S-transferase gene,Catalase gene,Toll-like receptor gene family,tyrosinase gene,discoidin domain receptor 1 gene,human leukocyte antigen alleles,superoxide dismutase gene,etc.To study the susceptible genes for vitiligo may provide a basis for the elucidation of vitiligo pathogenesis,and greatly benefit the early diagnosis,treatment and prevention of vitiligo,especially generalized vitiligo.

  12. Low-energy helium-neon laser therapy induces repigmentation and improves the abnormalities of cutaneous microcirculation in segmental-type vitiligo lesions.

    Science.gov (United States)

    Wu, Chieh-Shan; Hu, Stephen Chu-Sung; Lan, Cheng-Che E; Chen, Gwo-Shing; Chuo, Wen-Ho; Yu, Hsin-Su

    2008-04-01

    Segmental vitiligo (SV) is a special form of vitiligo occurring in a dermatomal distribution, and an abnormality involving the sympathetic nerves supplying the affected dermatome is known to underlie this disorder. Previously, we have shown that SV is associated with an abnormal increase in cutaneous blood flow and adrenoceptor responses in the affected areas. Since SV is resistant to conventional forms of therapy, its management represents a challenge for dermatologists. Low energy helium-neon lasers (He-Ne laser, wavelength 632.8 nm) have been employed as a therapeutic instrument in many clinical situations, including vitiligo management and repair of nerve injury. The purpose of this study was to evaluate the effectiveness and safety of He-Ne lasers in treating SV, and determine their effects on the repair of sympathetic nerve dysfunction. Forty patients with stable-stage SV on the head and/or neck were enrolled in this study. He-Ne laser irradiation was administered locally at 3.0 J/cm2 with point stimulation once or twice weekly. Cutaneous microcirculatory assessments in six SV patients were performed using a laser Doppler flowmeter. The sympathetic adrenoceptor response of cutaneous microcirculation was determined by measuring cutaneous blood flow before, during and after iontophoresis with sympathomimetic drugs (phenylephrine, clonidine and propranolol). All measurements of microcirculation obtained at SV lesions were simultaneously compared with contralateral normal skin, both before and after He-Ne laser treatment. After an average of 17 treatment sessions, initial repigmentation was noticed in the majority of patients. Marked repigmentation (> 50%) was observed in 60% of patients with successive treatments. Cutaneous blood flow was significantly higher at SV lesions compared with contralateral skin, but this was normalized after He-Ne laser treatment. In addition, the abnormal decrease in cutaneous blood flow in response to clonidine was improved by He

  13. An open labeled, comparative clinical study on efficacy and tolerability of oral minipulse of steroid (OMP alone, OMP with PUVA and broad / narrow band UVB phototherapy in progressive vitiligo

    Directory of Open Access Journals (Sweden)

    Rath Namita

    2008-01-01

    Full Text Available Background: Several modalities of treatment have been tried in vitiligo with varied results; however, Indian data on comparative studies of two or more therapies are limited. Aims: We compared different phototherapy methods with an oral steroid as an adjunct to determine the method with the best tolerability and efficacy. Methods: Eighty-six patients with progressive vitiligo were randomly assigned to different study groups according to a continuous selection method over a period of one year. Group 1 was given OMP + PUVA, group 2 OMP + UVB (NB, group 3 OMP + UVB (BB and group 4 was given OMP alone. Each patient was followed up for six months and then released from treatment. Clinical evaluation was made at the end of three and six months. Results: In group 1 (OMP + PUVA, marked improvement was seen in 18.51% while moderate improvement was seen in 66.66% of the patients. Marked improvement was seen in 37.03% in group 2 (OMP + NB-UVB while 44.44% had moderate improvement. In group 3 (OMP + BB UVB, 8.33% showed marked improvement while moderate improvement was seen in 25% of the patients. Marked and moderate improvement was seen in 5 and 10% of group 4 (OMP patients, respectively. Conclusions: Our study compared four treatment modalities in vitiligo patients, out of which oral minipulse of steroids (OMP only had an adjunct value and was not very effective by itself. Narrow band UVB has a definite edge over broad band UVB and should be preferred when both options are available. NB-UVB and PUVA showed comparable efficacy.

  14. 节段型白癜风的研究进展%Update on segmental vitiligo

    Institute of Scientific and Technical Information of China (English)

    毛秋霞; 贾苇雪; 肖学敏; 李诚让

    2015-01-01

    Segmental vitiligo,a subtype of vitiligo,is distributed segmentally along cutaneous nerves.Compared with other types of vitiligo,segmental vitiligo has distinctive clinical manifestations,pathogenesis and treatment.It is characterized by early onset,asymmetric skin lesions,rapid progression,and low likelihood of spreading to other sites or being accompanied by other autoimmune diseases.The etiology and pathogenesis of segmental vitiligo remain unclear,and several hypotheses have been put forward,such as the neural theory,genetic theory,immunological theory and a new three-step theory.The first-line treatment of segmental vitiligo is avoidance of causative factors,topical application of glucocorticoids,calcineurin inhibitors,vitamin D3 derivatives,and oral administration of a small dose of glucocorticoids at rapid progression stage.The second-line treatment is narrow-band ultraviolet B,excimer laser or helium-neon laser,etc.Surgery may serve as the third-line treatment when repigmentation is unsatisfying.%节段型白癜风是白癜风的一种亚型,沿皮神经节段分布.与其他类型白癜风相比,其在临床表现、发病机制和治疗等方面均有其自身特点.临床上具有发病早、皮损不对称、进展迅速的特点,很少向其他部位蔓延,很少伴有其他自身免疫病.病因及发病机制尚不明确,目前有神经学说、遗传学说、免疫学说及三步骤理论等.节段型白癜风的一线治疗是避免诱发因素,局部外用糖皮质激素、钙调神经磷酸酶抑制剂、维生素D3衍生物等,快速进展期可考虑系统给予小剂量糖皮质激素;二线治疗为窄谱中波紫外线、准分子激光或氦氖激光等;三线治疗是复色仍不理想,考虑手术治疗.

  15. A case of blue vitiligo%蓝色白癜风一例

    Institute of Scientific and Technical Information of China (English)

    韦旭华; 张江安; 于建斌; 李小红; 赵冰洁; 吕艳; 石卫华

    2016-01-01

    患者男,26岁,面部白斑10年余,局部变蓝灰7年。皮损变蓝灰前,曾因外用自配药物而发生接触性皮炎。皮肤科情况:左上唇、颞部有蓝灰色色素沉着,唇部皮损内可见白色毛发。皮肤镜:左上唇部可见蓝灰色斑,深浅不一,形状不规则,局部与白斑相间,白斑内见白色毛发残端。颞部组织病理:表皮局部黑素细胞减少,基底层、基底上层可见色素沉着,真皮浅层血管周围有少量慢性炎症细胞及嗜黑素细胞浸润,真皮汗管周围也见嗜黑素细胞浸润。诊断:蓝色白癜风。患者拒绝再治疗,仍在随访中。%A 26⁃year⁃old male patient presented with facial depigmented patches for 10 years, some of which turned to blue⁃grey 7 years prior to the presentation. Before the white patches turned blue⁃gray, the patient developed contact dermatitis due to topical application of self⁃made drugs. Skin examination showed blue⁃gray hyperpigmentation on the left upper lip and in the temporal region, with white hairs in the hyperpigmented lesions on the left upper lip. Dermoscopy revealed irregularly shaped, light to dark blue⁃gray patches on the left upper lip, which were intermingled with white patches in some regions, and white hair stubs were observed in the white patches. Histopathological examination of temporal lesions showed decreased melanocytes in some regions in the epidermis, pigmentation in both basal and suprabasal layers, perivascular infiltration of a small number of chronic inflammatory cells and melanophages in the superficial dermis, and melanophage infiltration around sweat ducts. Finally, the patient was diagnosed with blue vitiligo. He refused to receive any treatments, and follow⁃up was under way.

  16. Treating Vitiligo in Progression Stage With Traditional Chinese Medicine Anti-vitiligo Capsule Joint Stimulation Melanocyte Granules%中药克白抑扩胶囊联合促黑冲剂治疗进展期白癜风

    Institute of Scientific and Technical Information of China (English)

    薛长连; 张克凌; 路建伟

    2011-01-01

    Objective: To observed the clinical curative effect of treating vitiligo with traditional Chinese medicine " Anti-vitiligo Capsule" joint "Stimulation Melanocyte Granules" . Methods: The 80 cases of vitiligo in progression stage were divided randomly into two groups. The treating group had 40 cases treated by traditional Chinese medicine "Anti-vitiligo Capsule" joint "Stimulation Melanocyte Granules", and treated by the "Anti-vitiligo Tincture" for external application. The control group had also 40 cases treated by "Anti-vitiligo Capsule", and treated by the "Anti-vitiligo Tincture" for external application. Result: The total effective rate of treating group and control group were 97.1% and 96.2%. There are no significant Different between the two group therapeutic effect ( P>0.001 ). The total recovery rate of treating group and control group were 48.6% and 36.4%. There are significantly different between the two group therapeutic effect ( P<0.001 ). Conclusion: The result suggested that traditional Chinese medicine "Anti-vitiligo Capsule" joint "Stimulation Melanocyte Granules" is better for treating vitiligo.%目的:观察中药克白抑扩胶囊联合促黑冲剂治疗白癜风的进展期疗效.方法:对80例进展期白癜风患者进行随机分组,治疗组40例口服中药克白抑扩胶囊联合促黑冲剂,克白酊外涂;对照组40例口服中药克白抑扩胶囊,克白酊外涂.结果:治疗组与对照组总有效率97.1%和96.2%,两组比较P>0.001,表明两组总有效率无明显差异.痊愈率分别为48.6%和36.4%,两组比较P<0.001,表明治疗组的痊愈率明显优于对照组.结论:中药克白抑扩胶囊联合促黑冲剂能够提高白癜风的痊愈率,治疗白癜风进展期疗效确切.

  17. Treatment of vitiligo vulgaris with the combination therapy of topical steroid and vitamin D3 compound

    Directory of Open Access Journals (Sweden)

    Yoko Konishi

    2012-06-01

    Full Text Available We reported here two cases of vitiligo vulgaris successfully treated with the combination therapy of topical steroid and vitamin D3 compound and currently maintained by vitamin D3 analog without any adverse effects: skin atrophy, striae or telangiectasia on the exposed areas. The best-known mechanism of topical vitamin D3 analog is the enhancement of keratinocytes differentiation and anti-proliferative effects. Vitamin D3 analog is also reported to suppress T-cell mediated immunity, T-cell skin recruitment, and skin infiltration via down-regulating cutaneous lymphocyte antigen expression. Furthermore, vitamin D3 compounds are known to influence melanocyte maturation and differentiation and also to up-regulate melanogenesis. Autoreactive lymphocytes against melanocytes are one of the causes. Topical vitamin D3 analog may control vitiligo itself, however stronger immunosuppressive effects of topical corticosteroid may contribute to rapid re-pigmentation suppressing auto-reactive lymphocytes. The topical combination therapy is a simple, effective and safe option for vitiligo vulgaris in sun-exposed areas.

  18. More than horse MoDeHe scurfpea fruit tincture treatment of vitiligo clinical curative effect observation%匹多莫德和补骨脂酊治疗白癜风的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    杨军; 施林林; 戚建明

    2012-01-01

    目的:观察匹多莫德口服液和补骨脂酊联合治疗白癜风的临床疗效.方法:对治疗组48例患者联合使用匹多莫德口服液和补骨脂酊,对照组48例患者使用补骨脂酊进行治疗.结果:治疗3个月后观察,治疗组的总有效率为66.67%,对照组的为29.17%,两组进行比较,差异具有显著性意义(P<0.05).结论:白癜风的发病与自身免疫功能紊乱有关,匹多莫德联合补骨脂酊可通过调节免疫功能发挥疗效,为白癜风患者带来新的希望.%Objective To observe more horse hogsmeade oral liquid and fructus psoraleae tincture combination therapy of vitiligo clinical curative effect. Method The treatment group in 48 patients combined use horse many hogsmeade oral liquid and fructus psoraleae tincture and control group in 48 patients use scurfpea fruit tincture treatment. Results Observation 3 months after treatment, the treatment group total effective rate is 66.67% and 29.17% in the control group, two groups of comparison, with significant difference significance (P < 0.05). Conclusions The pathogenesis of vitiligo and autoimmune disorders related, horse hogsmeade more joint scurfpea fruit tincture can adjust the immune function curative effect for patients with vitiligo bring new hope.

  19. To Observe the Clinical Curative Effect of NB-UVB Treatment of Vitiligo%观察窄谱中波紫外线治疗白癜风的临床疗效

    Institute of Scientific and Technical Information of China (English)

    于子红

    2016-01-01

    目的:窄谱中波紫外线治疗白癜风的临床疗效。方法方便选取2014年1—12月期间该院收治白癜风患者50例,以窄谱中波紫外线法对患者进行治疗,并将其设为观察组。同期选择白癜风患者50例,以氟轻松外涂治疗,并将其设为对照组。分析比较两组患者的临床治疗效果。结果12个月后对比疗效,观察组为84%,对照组为60%,组间差异有统计学意义(P<0.05)。同时,观察组患者治疗过程中未见明显不良反应,治疗安全性较高。结论窄谱中波紫外线治疗白癜风临床效果显著,安全性高,可在临床上进行推广应用。%Objective To observe the narrow-band UVB treatment of vitiligo clinical efficacy. Methods January 2014 to December,The hospital treated 50 patients with vitiligo, a narrow-band UVB treatment method for patients, and observation group. Choose the same period 50 patients with vitiligo, treatment with fluocinolone treated, and set the control group. Com-parative analysis of two clinical patient outcomes. Results To compare the efficacy of 12 months after the observation group was 84% vs 60%, with statistical significance (P<0.05) between groups. At the same time, the course of treatment in the observation group were no significant adverse reactions, the treatment of high security. Conclusion Narrow-band UVB treatment of vitiligo significant clinical effect, safe and can be popularized in clinical practice.

  20. Narrow Band Ultraviolet B Treatment for Human Vitiligo Is Associated with Proliferation, Migration, and Differentiation of Melanocyte Precursors.

    Science.gov (United States)

    Goldstein, Nathaniel B; Koster, Maranke I; Hoaglin, Laura G; Spoelstra, Nicole S; Kechris, Katerina J; Robinson, Steven E; Robinson, William A; Roop, Dennis R; Norris, David A; Birlea, Stanca A

    2015-08-01

    In vitiligo, the autoimmune destruction of epidermal melanocytes produces white spots that can be repigmented by melanocyte precursors from the hair follicles, following stimulation with UV light. We examined by immunofluorescence the distribution of melanocyte markers (C-KIT, DCT, PAX3, and TYR) coupled with markers of proliferation (KI-67) and migration (MCAM) in precursors and mature melanocytes from the hair follicle and the epidermis of untreated and narrow band UVB (NBUVB)-treated human vitiligo skin. NBUVB was associated with a significant increase in the number of melanocytes in the infundibulum and with restoration of the normal melanocyte population in the epidermis, which was lacking in the untreated vitiligo. We identified several precursor populations (melanocyte stem cells, melanoblasts, and other immature phenotypes), and progressively differentiating melanocytes, some with putative migratory and/or proliferative abilities. The primary melanocyte germ was present in the untreated and treated hair follicle bulge, whereas a possible secondary melanocyte germ composed of C-KIT+ melanocytes was found in the infundibulum and interfollicular epidermis of UV-treated vitiligo. This is an exceptional model for studying the mobilization of melanocyte stem cells in human skin. Improved understanding of this process is essential for designing better treatments for vitiligo, ultimately based on melanocyte stem cell activation and mobilization.

  1. 钙泊三醇倍他米松软膏治疗非泛发型白癜风的疗效观察%Effect of calcipotriol betamethasone ointment on treatment of non-generalized vitiligo

    Institute of Scientific and Technical Information of China (English)

    周隽; 李剑; 傅雯雯

    2013-01-01

    目的 观察钙泊三醇倍他米松软膏治疗非泛发型白癜风的临床疗效和安全性.方法 51例非泛发型白癜风患者外用钙泊三醇倍他米松软膏,每日1次涂于患处,连续使用3个月,每月1次观察临床疗效及不良反应.结果 51例患者中,痊愈8例,显效21例,好转10例,无效12例;显效率56.86%(29/51,复色面积≥50%),有效率76.47%(39/51,复色面积≥10%).结论 钙泊三醇倍他米松软膏治疗非泛发型白癜风安全、有效,不良反应小.%Objective To evaluate the therapeutic effect and safety of calcipotriol betamethasone ointment on the treatment of non-generalized vitiligo. Methods Calcipotriol betamethasone ointment was topically applied to the vitiliginous patches once daily in 51 patients with non-generalized vitiligo for 3 months, and the therapeutic effect and adverse effect were observed in each month. Results Of these 51 patients, 8 had complete repigmentation, 21 achieved excellent response, 10 experienced improvement, and the other 12 achieved almost no response. The excellent response rate was 56.86% (29/51, area of repigmentation ≥ 50% ), and the effective rate was 76.47% (39/51, area of repigmentation ≥ 10%). Conclusion Calcipotriol betamethasone ointment is effective and safe in the treatment of vitiligo, with less adverse effect.

  2. 护理干预在退白汤联合NB-UVB紫外线照射治疗寻常型白癜风中的应用%Application of nursing intervention for treating vltiligo vulgaris by Anti-Vitiligo Decoction associated with NB-UVB irradiation

    Institute of Scientific and Technical Information of China (English)

    黄红玫

    2011-01-01

    目的 探讨护理干预在退白汤联合NB-UVB紫外线照射治疗寻常型白癜风中的应用效果.方法 将178例寻常型白癜风患者按就诊顺序采用单双号抽签法分为实验组89例和对照组89例;实验组内服退白汤联合NB-UVB紫外线照射,同时配合适当的护理干预;对照组仅内服退白汤联合NB-UVB紫外线照射,两组患者疗程均为3个月.采用统一的疗效评定标准进行疗效和安全性评价.结果 实验组有效率75.28%,对照组有效率为60.67%,两组比较差异有统计学意义(x2=4.361 6,P<0.05);实验组治疗寻常型白癜风局限性疗效明显优于治疗散发性疗效,差异有统计学意义(85.42%比63.41%,x2=5.752 3,P<0.05);两组患者总不良反应发生率比较,差异无统计学意义(x2=0.0465,P>0.05).结论 退白汤内服联合NB-UVB紫外线照射并配合适当的护理干预是治疗寻常型白癜风的有效方法.%Objective To investigate the clinical effect of nursing intervention for treating vitiligo vulgaris by Anti-Vitiligo Decoction associated with NB-UVB irradiation.Methods 178 patients with vitiligo vulgaris were divided into two groups according to the treatment sequence balloting with odd and even numbers.89 patients in treating group and 89 patients in control group took Anti-Vitiligo Decoction associated with NB-UVB irradiation, besides that patients in treating group received the appropriated nursing intervention.The effectiveness and safety were appreciated by the standard of PASI.Results The effect ratio of the two groups were 75.28% and 60.67% respectively, and there was statistically significant difference between the two groups (x2 = 4.361 6,P < 0.05).In treating group, the effect of treating limited vitiligo vulgaris was significantly better than treating the scattered vitiligo vulgaris (85.42% vs 63.41%, x2 = 5.752 3, P < 0.05).The incidence of side effects was similar in the two groups (x2 = 0.046 5, P > 0.05).Conclusions It is a

  3. Dysfunction of Autophagy: A Possible Mechanism Involved in the Pathogenesis of Vitiligo by Breaking the Redox Balance of Melanocytes.

    Science.gov (United States)

    Qiao, Zhuhui; Wang, Xiuxiu; Xiang, Leihong; Zhang, Chengfeng

    2016-01-01

    Vitiligo is a common chronic acquired pigmentation disorder characterized by loss of functional melanocytes from the epidermis and follicular reservoir. Among multiple hypotheses which have been proposed in the pathogenesis of vitiligo, autoimmunity and oxidative stress-mediated toxicity in melanocytes remain most widely accepted. Macroautophagy is a lysosome-dependent degradation pathway which widely exists in eukaryotic cells. Autophagy participates in the oxidative stress response in many cells, which plays a protective role in preventing damage caused by oxidative stress. Recent studies have enrolled autophagy as an important regulator in limiting damage caused by UV light and lipid oxidation, keeping oxidative stress in a steady state in epidermal keratinocytes and maintaining normal proliferation and aging of melanocytes. Impairment of autophagy might disrupt the antioxidant defense system which renders melanocytes to oxidative insults. These findings provide supportive evidence to explore new ideas of the pathogenesis of vitiligo and other pigmentation disorders.

  4. 儿童白癜风治疗进展%Update on the vitiligo treatment in children

    Institute of Scientific and Technical Information of China (English)

    王林; 曹燕; 贾虹; 杨雪源

    2016-01-01

    There are many treatment modalities for vitiligo, but the treatment is generally more effective in children than in adult. The aim in the treatment of vitiligo is to control the progression and to repigment the lesions. The treatment of vitiligo in children was updated in this paper.%白癜风治疗方法很多,但一般而言,儿童及青少年患者治疗效果要优于成人。白癜风治疗的关键在于稳定疾病进展、促使皮损复色,本文对临床常用治疗儿童白癜风的方法进行了综述。

  5. Retrospective Survey and Analysis on Vitiligo Diagnosed by Uygur Medicine%维吾尔医诊断白癜风回顾性调查分析

    Institute of Scientific and Technical Information of China (English)

    吐尔洪·艾买尔; 居来提·托合提; 斯拉甫·艾白; 艾合买提江·买买提; 玉素甫·买提努尔; 肖开提·阿不都拉; 茹仙古丽·沙吾尔; 库尔班·艾力

    2011-01-01

    Objective To review the vitiligo treatment materials of five Xinjiang Uygur medical hospitals in recent 10 years, summarize and analyze of the regularity of distribution and pathogenesis, and methods and principles of Uygur medical diagnosis. Methods Five hundred vitiligo cases diagnosed and hospitalized by five Xinjiang Uygur medical hospital from 1998 to 2008 were retrieved. Based on the demographic data and diagnostics material, the clinical diagnosis materials were analyzed and summarized. Results 362 of the 500 cases met the standard. Age, gender, the abnormal change of Hilit tabiyi {normal body fluids),physical examination and other infection, environment, mental, genetic factors were related indirectly with the disease. Uygur medical syndrome differentiation, distribution of symptoms, pathogenic site, differential diagnosis all had its own characteristics. Conclusion Age, gender, marriage, skin sensitivity, degree of education, nutritional status and syndrome differentiation of uygur medicine have the different diagnostic characters. The patients with vitiligo, caused by Hilit ghayri tabiyi (abnormal body fluids), has different mizaj (temperament), and show different signs, symptoms and vitiligo characteristic symptoms, which highlights the type classification of Hilit ghayri tabiyi and the characteristics of individualized diagnosis method. Correct conclusion of syndrome differentiation of Uygur medicine can play important role in the judgement of patient's condition and diagnosis.%目的 回顾新疆5家维吾尔医院10年以来白癜风治疗资料,总结分析白癜风在人群中的分布、发病规律及维吾尔医诊断原则和方法等.方法 检索1998-2008年新疆5家维吾尔医医院确诊为白癜风并接受住院治疗的500例患者住院病历,以人口学资料、诊断学资料为主要调查内容,进行回顾性调查、分析.结果 收集到的500例住院病历中有362例达到纳入标准,年龄、性别、合立体台比依(

  6. 白癜风皮损中非黑素细胞进展%Non-melanocytes in vitiligo lesions

    Institute of Scientific and Technical Information of China (English)

    金永红; 许爱娥

    2008-01-01

    The pathogenesis of vitiligo is still unclear.In addition to melanocytes,non-melanocytes are involved in the pathogenesis of vitiligo.More and more studies have been conducted on non.melanocytes in vitiligo recently.It has been shown that keratinocytes,Langerhans cells and fibroblasts affect the migration,proliteration and differentiation of melanocytes.The changes of cytokines secreted by and ultrastructural abnormality of non-melanocytes may affect the activity and apoptosis of melanocytes in vitiligo lesions,influence pigrnentogenesis in skin,thereafter,take part in the pathogenesis of vitiligo.This paper presents the latest investigation of keratinocytes,Langerhans cells and fibroblasts in vitiligo.%白癜风的发病机制尚未完全明了.其发病除黑素细胞异常外,还涉及非黑素细胞的变化,近年来非黑素细胞在白癜风中的研究逐渐增多.研究表明,一些皮肤非黑素细胞如角质形成细胞、朗格汉斯细胞、成纤维细胞等与黑素细胞关系密切,这些细胞影响黑素细胞的迁移、增殖、分化等功能.白癜风皮损中非黑素细胞超微结构的异常和分泌细胞因子的变化可影响黑素细胞的活性及凋亡,影响皮肤色素生成,从而参与白癜风发病.

  7. Advances in animal models of vitiligo%白癜风动物模型的研究进展

    Institute of Scientific and Technical Information of China (English)

    祝逸平; 许爱娥

    2013-01-01

    白癜风是一种皮肤黏膜色素脱失性疾病,其病因及发病机制尚未明确.多种常规应用模式的白癜风动物模型存在局限性.随着现代科学技术的发展,许多新型白癜风动物模型的建立,如酪氨酸酶相关、CD8+T细胞相关和热休克蛋白70相关的白癜风动物模型等,这些模型与人白癜风相类似度、重复性和专一性等方面都有一定程度的提高,成为研究白癜风发病机制,治疗靶点和治疗药物等方面研究的主要基础.%Vitiligo is an autoimmune hypopigmented disorder of the skin and mucosa with unclear etiology and pathogenesis.There are a variety of animal models for vitiligo,but most of them have limitations.With the development of modern science and technology,many new animal models have been established for vitiligo,such as animal models of vitiligo related to tyrosinase,CD8+ T cells,heat shock protein (HSP) 70,and so on.The similarity to human vitiligo,reproducibility and specificity have been increased to some extent in these novel models,which may provide a basis for further studies into vitiligo,including the pathogenesis,therapeutic targets and medicines,and so on.

  8. 儿童白癜风与甲状腺功能指标异常及其他免疫性疾病的关系%Abnormality of parameters of thyroid function and incidence of autoimmune diseases in children with vitiligo

    Institute of Scientific and Technical Information of China (English)

    杨芸; 骆肖群; 傅雯雯

    2009-01-01

    目的 探讨儿童白癜风与甲状腺功能指标异常及其他免疫性疾病的关系.方法 对363例白癜风儿童(男198例,女165例)和93例对照儿童(男55例,女38例)进行甲状腺功能指标的检查.结果 363例白癜风儿童中有43例(11.8%)儿童有不同程度的甲状腺功能指标的异常,93例对照组正常儿童中有4例儿童甲状腺功能指标异常,两者比较差异有统计学意义.白癜风儿童甲状腺功能指标异常发生率明显增高(P<0.05).而43例甲状腺功能异常的白癜风儿童中,寻常型白癜风儿童为39例(13.6%),节段型白癜风儿童为4例(5.3%),寻常型比节段型白癜风儿童甲状腺功能指标异常发生率有明显增高(P<0.05).结论 儿童寻常型白癜风患者的甲状腺功能指标异常的发生率明显增高.%Objective To investigate the abnormality of parameters of thyroid function and incidence of autoimmune diseases in children with vitiligo.Methods A total of 363 children with vitiligo,including 198 males and 165 females were recruited into this study together with 93 normal human controls(55 males and 38 females).The serum levels of free tetraiodothyronine,free triiodothyronine,thyroid stimulating hormone,antithyroperoxidase antibody and thyroglobulin antibody were determined by chemiluminescent immunoassay in these subiects.Results The abnormality of parameters of thyroid function was observed in 43 out of 363(11.8%)patients affected by vitiligo and in 4 out of 93(4.3%)normal human controls;a significant difference was observed between the two groups (P<0.05).Of the 43 patients wim abnormality of parameters of thyroid function,39 were diagnosed as vitiligo vulgaris,4 as segmental vitiligo.A significant increase Was observed in the incidence of abnormality of parameters of thyroid function in patients with vitiligo vulgaris compared with those with segmental vitiligo(P<0.05).Conclusion There is an increase in tbe abnormality of parameters of thyroid

  9. 自体黑素细胞培养移植治疗白癜风的临床研究%Clinical research of autologous melanocytes culture and transplantation for the treatment of vitiligo

    Institute of Scientific and Technical Information of China (English)

    罗卫; 马春林; 吕俊卿; 蔡瑞康

    2012-01-01

    目的 进一步研究和探索黑素细胞培养移植治疗白癜风的方法 和疗效.方法 从发疱壁上获取黑素细胞,行纯黑素细胞培养与增殖,采用移植区刮除种植法行自体黑素细胞培养移植治疗白癜风.结果 本组18例白癜风患者(21块皮损)进行了自体黑素细胞培养移植,总有效率90.48%.结论 此方法较简单,可治疗面积大,治疗后色素分部均匀,值得临床推广和应用.%Objective To explore the method and efficacy of the autologous melanocytes culture and transplantation for the treatment of vitiligo. Methods The melanocytes obtained from suction blisters wall and then culture and proliferation the melanocytes. The vitiliginous lesion is scratched and then transplanted the cultured melanocytes. Results Totally 21 lesions of 18 patients with vitiligo were performed the treatment of autologous melanocytes culture and transplantation, and the total effective rate was 90. 48% . Conclusion It is an optimal approach of autologous melanocytes culture and transplantation for the treatment of vitiligo, which is easy to perform that could cure bigger area with equally distributed pigment. It is worth spreading in clinic.

  10. AHR promoter variant modulates its transcription and downstream effectors by allele-specific AHR-SP1 interaction functioning as a genetic marker for vitiligo.

    Science.gov (United States)

    Wang, Xiaowen; Li, Kai; Liu, Ling; Shi, Qiong; Song, Pu; Jian, Zhe; Guo, Sen; Wang, Gang; Li, Chunying; Gao, Tianwen

    2015-09-15

    Vitiligo is an acquired depigmentation disorder largely caused by defective melanocyte- or autoimmunity-induced melanocyte destruction. The aryl hydrocarbon receptor (AHR) is essential for melanocyte homeostasis and immune process, and abnormal AHR was observed in vitiligo. We previously identified the T allele of AHR -129C > T variant as a protective factor against vitiligo. However, biological characterization underlying such effects is not fully certain, further validation by mechanistic research is warranted and was conducted in the present study. We showed that -129T allele promoted AHR transcriptional activity through facilitating its interaction with SP1 transcription factor (SP1) compared with -129C allele. We subsequently found reduced peripheral AHR and SP1 transcript expressions in vitiligo and a negative correlation of AHR level with disease duration. We also investigated AHR-related cytokines and observed increased serum TNF-α concentration and diminished serum levels of IL-10 and TGF-β1 in vitiligo. Further genetic analysis showed that -129T carriers possessed higher levels of AHR and IL-10 than -129C carriers. Therefore, our study indicates that the modulation of AHR transcription by a promoter variant has a profound influence on vitiligo, not only advancing our understanding on AHR function but also providing novel insight into the pathogenesis of degenerative or autoimmune diseases including vitiligo.

  11. New progress in genetic background of vitiligo immunology%白癜风免疫学遗传背景新进展

    Institute of Scientific and Technical Information of China (English)

    王倩倩; 项蕾红

    2014-01-01

    近来白癜风致病基因被证实可以调控与白癜风相关自身免疫性疾病的发病,这说明白癜风与其他自身免疫性疾病发病密切关联,它们有共同的致病因素,受共同易感基因调控。因此探讨白癜风免疫学遗传背景可以为白癜风的治疗提供良好的科研基础。%Recently, vitiligo pathogenic genes were confirmed to regulate the incidence of vitiligo associated autoimmune diseases, which indicated that vitiligo and some other autoimmune diseases may be closely related. Common pathogenetic factors and predisposing genes regulation may affect both vitiligo and vitiligo associated autoimmune diseases. Therefore, the investigation of the genetic background of vitiligo immunology will provide good scientific research basement for the vitiligo treatment.

  12. Demonstration of tyrosinase in the vitiligo skin of human beings by a sensitive fluorometric method as well as by 14C(U)-L-tyrosine incorporation into melanin

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    Husain, I.; Vijayan, E.; Ramaiah, A.; Pasricha, J.S.; Madan, N.C.

    1982-03-01

    Tyrosinase activity (Monophenol, dihydroxyphenylalanine: oxygen oxidoreductase EC 1.14.18.1) in vitiligo and normal epidermal homogenates of skin from human beings was measured by estimating beta 3,4-dihydroxyphenylalanine (dopa) by a highly sensitive fluorometric method described in this paper. The tyrosine activity in the vitiligo skin was about 4 to 37% of corresponding normal skin. The activity of tyrosinase in normal human skin from different individuals and from different regions of the body was in the range of 4 to 140 picomoles of beta 3,4-dihydroxyphenylalanine formed per min/mg protein of epidermal homogenate. The enzyme from vitiligo and normal skin was severely inhibited by substance(s) of low molecular weight. The enzyme exhibits a lag of about 4 hr in the absence of added beta 3,4-dihydroxyphenylalanine and 1 hr in presence of 5 microM dopa. Tyrosinase from the normal and vitiligo skin was inhibited by excess concentration of tyrosine. The homogenates from vitiligo skin could synthesize melanin from C14(U)-L-Tyrosine. The rate of tyrosine incorporation into melanin by the epidermal homogenates is increased by 3,4-dihydroxyphenylalanine (dopa) disproportionate to its effect on tyrosinase activity. Based on the data presented in this paper it is concluded that melanocytes are present in the vitiligo skin. A tentative hypothesis is put forward to explain the lack of melanin synthesis by the vitiligo skin under in vivo conditions, although melanocytes are present.

  13. Vitiligo: A quantitative analysis of the world research output during 2001-2012

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    Raj Kumar Bhardwaj

    2013-01-01

    Full Text Available The study aimed to evaluates vitiligo research carried out in different countries of the world during 2001-2012 using bibliometric indicators. Data of the present study has been obtained from Scopus (http://www.scopus database using the keywords "Alopecia universalis" "onychodystrophy" "Schrander-Stumpel Theunissen Hulsmans syndrome", "Spastic paraplegia", "Pigmentation Disorders", "Hypopigmentation" during the period of study. The study examined the type of documents, country-wise contribution, subject-wise distribution, most productive institutions, most productive authors, highly productive journals and highly cited papers in vitiligo research. A total of 4091 papers were published during 2001-2012 at the annual growth rate of (20.24%. USA is the most productive country which shared (19.41% of the world publications. India holds 2 nd rank with 388 (9.48% papers. Overall, 50 or more citations have been witnessed to 207 papers. Germany, France, Spain and Japan activity index has been come down during the period of 2007-2012. However, majority of leading institutions are located in developed countries such as USA, UK, France and Germany. Nevertheless, Postgraduate Institute of Medical Education and Research, Chandigarh has been identified the most productive institution in vitiligo research. Besides this, Karin Uta Schallreuter from University of Bradford is found the most productive author who has contributed 45 articles. Journal of the European Academy of Dermatology and Venereology′ is identified the most productive journal. In the last two decades considerable progress has been made to cope this affliction, nevertheless scientist are not able to develop a vaccine. It is expected that in near future a vaccine will be available in the market to counter the disease.

  14. 自拟中药退白汤联合窄谱紫外线照射治疗白癜风53例观察%Traditional Chinese Medicine Decoction Combined with Narrow-band Ultraviolet Irradiation Annealing Treatment of Vitiligo Clinical Observation of 53 Cases

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    严霞; 顾锐龙; 谢勇

    2014-01-01

    目的探讨中药退白汤联合窄谱紫外线治疗寻常型白癜风的临床疗效。方法我们于2012年9月~2014年4月运用自拟的中药退白汤内服,联合全身窄谱紫外线(NB-UVB)局部照射治疗寻常性白癜风53例,对照组给予窄谱紫外线照射,治疗组内服退白汤,并给予窄谱紫外线照射。结果联合治疗组有效率73.58%,明显优于对照组的有效率51.22%。结论退白汤联合NB-UVB治疗稳定期寻常型白癜风安全、有效,尤其适用于局限性白癜风。%Objective To explore the clinical curative ef ect of traditional Chinese medicine decoction combined with narrow band ultraviolet back in the treatment of vitiligo vulgaris. Methods We in 2012 September~2014 April since the use of traditional Chinese medicine decoction oral y taken back, combined with systemic narrow spectrum ultraviolet (NB-UVB) in 53 cases of the treatment of vitiligo vulgaris local irradiation, the control group was given the narrow spectrum ultraviolet irradiation, patients in the treatment group took back soup, and give the narrow spectrum ultraviolet irradiation. Results The ef ective rate of treatment group was 73.58%, significantly bet er than the control group on the ef iciency of 51.22%. Conclusion The decoction combined with NB-UVB in the treatment of stable phase back vitiligo vulgaris is safe and ef ective, especial y for the limitations of vitiligo.

  15. Narrowband Ultraviolet B Treatment in Skin Diseases Beyond Vitiligo, Psoriasis and Mycosis Fungoides

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    Dilek Seçkin

    2009-09-01

    Full Text Available Narrowband ultraviolet B, with its nonspecific immunmodulatuar actions, is a potential treatment alternative in many skin diseases. Various studies have shown its effects in psoriasis and vitiligo, treatment protocols have been developed especially for psoriasis. Apart from these, narrowband ultraviolet B is used as first-line therapy in skin conditions that are common in clinical practice. However, its efficacies in such diseases are not very well-known. The effects of narrowband ultraviolet B in atopic dermatitis, pityriasis lichenoides, lichen planus, pruritus, chronic urticaria, symptomatic dermographism and polymorphic light eruption are discussed here in light of the literature data.

  16. Application of Epidemiology and Statistical methods in understanding disease aetiology-study on Vitiligo.

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    Bharat Bhavsar

    2010-07-01

    Full Text Available The disease results from the interaction between the person’s genetic make up and his outer environment.1 The proportionate role of both the factors varies from disease to disease. There are some diseases with unclear aetiology; Vitiligo is one of them. The purpose of this paper is to show the application of Epidemiology and the statistical methods to understand the disease causation. The epidemiological study of skin conditions among school children in Urban and Rural areas of Surat district carried out by the author 2,3 brought out following observations........

  17. Syndrome Differentiation and Treatment Experience of Vitiligo%白癜风证治经验

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    周宝宽

    2011-01-01

    Objective:To summarize syndrome differentiation and treatment experience of vitiligo in clinic. Methods:Discuss etiopathogenisis , pathogenesis, pattern of syndrome, method of treatment, formulas and medicinals based on medical records. Results:Etiopathogenisis and pathogenesis are natural endowment weakness, being invaded by exogenous pathogen, blocking meridian, Qi and blood to be on bad terms; moodiness internal injury, depression of liver-QI, stagnation of liver-QI, disease secondly induced by wind evil, meridian blocked, failure of skin and muscle to be nourished.Cmmon Syndromes of Vitiligo are QI-blood disharmony, stagnation of QI due to depression of the liver, hepatic and renal yin deficiency, stagnation of vital energy and blood stasis.Treatment principles of vitiligo are coordinating qi and blood, disperse the depressed liver-energy and regulating vital energy, nourishing liver and kidney, activating blood circulation to dissipate blood stasis, dispelling wind and removing obstruction in the meridians. Conclusions:Syndrome differentiation and treatment shows a better therapeutic effect for vitiligo.%目的:总结周宝宽主任医师辨证论治白癜风经验.方法:以医案的形式阐述白癜风的病因病机、证型、治法、方药.结果:白癜风的病因病机为禀赋不耐,感受外邪,阻滞经络,气血失和;情志内伤,肝气郁结,复感风邪,肌肤失养;禀赋不足,肝肾亏虚,久病失治或复感风邪,脉络瘀滞,肌肤失养.常见证型为气血失和、肝郁气滞、肝肾阴虚、气滞血瘀.常用治法为调和气血、疏肝理气、滋补肝肾、活血化瘀、祛风通络.结论:辨证论治白癜风有一定疗效.

  18. Expression of NF-E2 related factor in vitiligo lesions%白癜风患者皮损NF-E2相关因子2的表达

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    关翠萍; 尉晓冬; 周妙妮; 李永伟; 赵德矿; 许爱娥

    2008-01-01

    Objective To investigate the level of NF-E2 related factor 2 (Nrf 2) in vitiligo lesions.Methods Tissue samples were obtained by press suction blisters at lesional and donor sites of 12 patients with vitiligo who were managed with epidermal transplantation. Four lesional samples from the patients were subjected to primary culture and the level of Nrf 2 was detected by AEC immunohistochemistry after 48hours of culture. Western blotting was utilized to further detect the level of cytoplasmic and nuclear Nrf 2 in tissue samples from the other 8 patients with vitiligo. Results Immunohistochemistry revealed that Nrf 2 was predominantly expressed in cytoplasm, rather than nuclei, of keratinocytes in vitiligo lesions compared with the normal skin of patients. The level of nuclear Nrf 2 was significantly lower in lesions than that in normal skin (0.10 ± 0.03 vs 0.26 ± 0.03, P < 0.01) of the patients. In contrast, there was no significant dif- ference in the level of cytoplasmic Nrf2 between lesional and normal skin (0.61 ± 0.03 vs 0.60 ~ 0.02, P >0.05) of patients. Conclusion These results reveal an abnormality of nuclear translocation of Nrf 2 in vitili-go lesions.%目的 分析白癜风患者皮损处Nrf2表达水平.方法 采用AEC免疫组化法检测4例表皮移植的寻常型白癜风患者皮损处Nrf 2的表达,蛋白印迹分析表皮移植的8例寻常型白癜风患者皮损组织和其正常供皮处组织细胞核和细胞质中Nrf 2的表达水平,并进行统计学差异分析.结果 与白癜风患者正常供皮处组织相比,皮损处Nrf 2的表达多数集中在角质形成细胞的胞质中,细胞核中表达量非常低.蛋白印迹结果表明,白癜风患者皮损处核蛋白中Nrf 2表达水平(0.10 ±0.03)显著低于正常部位(0.26 ±0.03,P<0.01),胞质蛋白中皮损处Nrf 2表达水平(0.61±0.03)与正常部位(0.60 ±0.02)差异无统计学意义(P>0.05).结论 白癜风患者皮损处存在Nrf 2核转位异常.

  19. 308 nm准分子激光联合胡椒碱治疗白癜风疗效观察%Efficacy of 308 nm excimer laser plus Piperine in the treatment of vitiligo

    Institute of Scientific and Technical Information of China (English)

    刘畅; 吴一菲

    2016-01-01

    目的:评价308nm准分子激光联合胡椒碱对白癜风的临床疗效和安全性。方法将入选的87例白癜风患者按就诊顺序随机分为2组,共80例患者完成试验观察,治疗组予308nm准分子激光局部照射每周2次,联合胡椒碱酊局部外用2次/d治疗,对照组予308nm准分子激光局部照射每周2次,联合8-甲氧基补骨脂素(8-MOP)溶液局部外用2次/d治疗。治疗时间为4周一疗程,治疗3个疗程,比较2组患者在白癜风皮损复色上的差异。结果治疗组的显效率90%,对照组为77.5%。2组显效率差异有统计学意义(P<0.05),治疗中未出现严重的不良反应。结论308nm准分子激光联合胡椒碱对于治疗白癜风有明显的效果,且无明显不良反应,对白癜风的治疗是一个很好的选择。%Objective To investigate the curative effect and security of the 308nm excimer laser combined with Piperine for vitiligo. Methods eighty-sev-en patients with vitiligo were randomly divided into two groups,and there were eighty patients complete the observation,the patients in treatment group were treated twice weekly with 308nm excimer laser combined with topical application of Piperine twice daily,the patients in control group were treated twice week-ly with 308nm excimer laser combined with topical application 8-MOP twice daily. Four weeks constituted a course of treatment , after 3 courses treatment, the pigmentation level of vitiligo lesions were compared between the 2 groups of patients. Results The total excellence rate was 90%, 77. 5% in treatment group ,and control group respectively, which showed significant difference between the two groups (P<0. 05). No serious adverse events were recorded in the two groups. Conclusion The combination of 308nm excimer laser with topical Piperine for the treatment of vitiligo has obvious effect, and no obvious ad-verse reactions, which is a very good choice for the treatment of vitiligo.

  20. Association of HLA-DQA1*0302, DQB1*0303 alleles with vitiligo in Uygur nationality in Xinjiang Uygur Autonomous Region%HLA-DQA1*0302、DQB1*0303与新疆维吾尔族白癜风的相关性研究

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    辛宁; 唐小辉; 陈军; 康晓静

    2011-01-01

    目的 探讨HLA-DQA1、DQB1等位基因与新疆维吾尔族白癜风相关性。方法 聚合酶链反应-序列特异性引物(PCR-SSP)检测300例维吾尔族白癜风患者HLA-DQA1*0302、DQB 1*0303等位基因。结果 与300例维吾尔族正常人对照组相比,①白癜风患者DQAI *0302(20.5%比13.83%)、DQB1*0303(30.17%比13.33%)等位基因频率显著增高(P<0.01);②HLA-DQA1*0302、DQB1 *0303等位基因频率在成人型(发病年龄> 12岁)及儿童型(发病年龄≤12岁)的白癜风患者中均增高(P<0.01);③HLA-DQB1*0303等位基因频率在有、无家族史的白癜风患者中均增高(P<0.01),HLA -DQA 1*0302等位基因频率在无家族史病例中显著增高(P<0.01);④白癜风组儿童型和成人型两组间比较及有、无家族史两组间比较,DQA1*0302、DQB1 *0303等位基因频率差异无统计学意义(P>0.05)。结论 HLADQA1*0302、DQB1 *0303等位基因可能与新疆维吾尔族白癜风相关,儿童型和成人型及有、无家族史的白癜风在其遗传背景上可能存在异质性。%Objective To identify the association of HLA-DQA1*0302 and DQB1*0303 alleles with vitiligo in Uygur nationality in Xinjiang Uygur Autonomous Region. Methods Polymerase chain reaction with sequence-specific primers (PCR-SSP) was performed to analyze the distribution of HLA-DQA1*0302 and HLA-DQB1*0303 alleles among 300 patients with vitiligo and 300 normal human controls of Uygur nationality in Xinjiang region. Results A significant increase was observed in the frequency of HLA-DQA1*0302 and -DQB1*0303 alleles in patients with vitiligo compared with the controls (20.5% vs. 13.83%, 30.17% vs. 13.33%, both P < 0.01 ). Increas