Bremsstrahlung-Based Imaging and Assays of Radioactive, Mixed and Hazardous Waste

Science.gov (United States)

Kwofie, J.; Wells, D. P.; Selim, F. A.; Harmon, F.; Duttagupta, S. P.; Jones, J. L.; White, T.; Roney, T.

2003-08-01

A new nondestructive accelerator based x-ray fluorescence (AXRF) approach has been developed to identify heavy metals in large-volume samples. Such samples are an important part of the process and waste streams of U.S Department of Energy sites, as well as other industries such as mining and milling. Distributions of heavy metal impurities in these process and waste samples can range from homogeneous to highly inhomogeneous, and non-destructive assays and imaging that can address both are urgently needed. Our approach is based on using high-energy, pulsed bremsstrahlung beams (3-6.5 MeV) from small electron accelerators to produce K-shell atomic fluorescence x-rays. In addition we exploit pair-production, Compton scattering and x-ray transmission measurements from these beams to probe locations of high density and high atomic number. The excellent penetrability of these beams allows assays and images for soil-like samples at least 15 g/cm2 thick, with elemental impurities of atomic number greater than approximately 50. Fluorescence yield of a variety of targets was measured as a function of impurity atomic number, impurity homogeneity, and sample thickness. We report on actual and potential detection limits of heavy metal impurities in a soil matrix for a variety of samples, and on the potential for imaging, using AXRF and these related probes.

Radioactive air emissions from non-uranium mining operations

International Nuclear Information System (INIS)

Silhanek, J.S.; Andrews, V.E.

1981-01-01

Section 122 of the Clean Air Act Amendments of 1977, Public Law 9595, directed the Administrator of the Environmental Protection Agency to review all relevant information and determine whether emissions of radioactive pollutants into ambient air will cause or contribute to air pollution which may reasonably be anticipated to endanger public health. A section of this document presented a theoretical analysis of the radioactive airborne emissions from several non-uranium mines including iron, copper, zinc, clay, limestone, fluorspar, and phosphate. Since 1978 EPA's Las Vegas Laboratory has been gathering field data on actual radionuclide emissions from these mines to support the earlier theoretical analysis. The purpose of this paper is to present the results of those field measurements in comparison with the assumed values for the theoretical analysis

Survey on non-nuclear radioactive waste

International Nuclear Information System (INIS)

2003-11-01

On request from the Swedish Radiation Protection Authority, the Swedish government has in May 2002 set up a non-standing committee for non-nuclear radioactive waste. The objective was to elaborate proposals for a national system for the management of all types of non-nuclear radioactive wastes with special consideration of inter alia the polluter pays principle and the responsibility of the producers. The committee will deliver its proposals to the government 1 December 2003. SSI has assisted the committee to the necessary extent to fulfill the investigation. This report is a summery of SSI's background material concerning non-nuclear radioactive waste in Sweden

Development and application of a fluorescent glucose uptake assay for the high-throughput screening of non-glycoside SGLT2 inhibitors.

Science.gov (United States)

Wu, Szu-Huei; Yao, Chun-Hsu; Hsieh, Chieh-Jui; Liu, Yu-Wei; Chao, Yu-Sheng; Song, Jen-Shin; Lee, Jinq-Chyi

2015-07-10

Sodium-dependent glucose co-transporter 2 (SGLT2) inhibitors are of current interest as a treatment for type 2 diabetes. Efforts have been made to discover phlorizin-related glycosides with good SGLT2 inhibitory activity. To increase structural diversity and better understand the role of non-glycoside SGLT2 inhibitors on glycemic control, we initiated a research program to identify non-glycoside hits from high-throughput screening. Here, we report the development of a novel, fluorogenic probe-based glucose uptake system based on a Cu(I)-catalyzed [3+2] cycloaddition. The safer processes and cheaper substances made the developed assay our first priority for large-scale primary screening as compared to the well-known [(14)C]-labeled α-methyl-D-glucopyranoside ([(14)C]-AMG) radioactive assay. This effort culminated in the identification of a benzimidazole, non-glycoside SGLT2 hit with an EC50 value of 0.62 μM by high-throughput screening of 41,000 compounds. Copyright © 2015 Elsevier B.V. All rights reserved.

SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes.

Science.gov (United States)

Tien, Wei-Ping; Lim, Gareth; Yeo, Gladys; Chiang, Suzanna Nicole; Chong, Chee-Seng; Ng, Lee-Ching; Hapuarachchi, Hapuarachchige Chanditha

2017-09-19

The monitoring of vectors is one of the key surveillance measures to assess the risk of arbovirus transmission and the success of control strategies in endemic regions. The recent re-emergence of Zika virus (ZIKV) in the tropics, including Singapore, emphasizes the need to develop cost-effective, rapid and accurate assays to monitor the virus spread by mosquitoes. As ZIKV infections largely remain asymptomatic, early detection of ZIKV in the field-caught mosquitoes enables timely implementation of appropriate mosquito control measures. We developed a rapid, sensitive and specific real-time reverse transcription polymerase chain reaction (rRT-PCR) assay for the detection of ZIKV in field-caught mosquitoes. The primers and PCR cycling conditions were optimized to minimize non-specific amplification due to cross-reactivity with the genomic material of Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, Culex tritaeniorhynchus, Culex sitiens and Anopheles sinensis, as well as accompanying microbiota. The performance of the assay was further evaluated with a panel of flaviviruses and alphaviruses as well as in field-caught Ae. aegypti mosquitoes confirmed to be positive for ZIKV. As compared to a probe-based assay, the newly developed assay demonstrated 100% specificity and comparable detection sensitivity for ZIKV in mosquitoes. Being a SYBR Green-based method, the newly-developed assay is cost-effective and easy to adapt, thus is applicable to large-scale vector surveillance activities in endemic countries, including those with limited resources and expertise. The amplicon size (119 bp) also allows sequencing to confirm the virus type. The primers flank relatively conserved regions of ZIKV genome, so that, the assay is able to detect genetically diverse ZIKV strains. Our findings, therefore, testify the potential use of the newly-developed assay in vector surveillance programmes for ZIKV in endemic regions.

Assay for dihydroorotase using high-performance liquid chromatography with radioactivity detection

International Nuclear Information System (INIS)

Mehdi, S.; Wiseman, J.S.

1989-01-01

An assay for measuring dihydroorotase activity was devised. Radiolabeled substrate and product were separated by high-performance liquid chromatography using a reverse-phase column with ion-pairing, and the radioactivity was quantitated by flow detection

Radioactive waste package assay facility. Volume 3. Data processing

International Nuclear Information System (INIS)

Creamer, S.C.; Lalies, A.A.; Wise, M.O.

1992-01-01

This report, in three volumes, covers the work carried out by Taylor Woodrow Construction Ltd, and two major sub-contractors: Harwell Laboratory (AEA Technology) and Siemens Plessey Controls Ltd, on the development of a radioactive waste package assay facility, for cemented 500 litre intermediate level waste drums. Volume 3, describes the work carried out by Siemens Plessey Controls Ltd on the data-processing aspects of an integrated waste assay facility. It introduces the need for a mathematical model of the assay process and develops a deterministic model which could be tested using Harwell experimental data. Relevant nuclear reactions are identified. Full implementation of the model was not possible within the scope of the Harwell experimental work, although calculations suggested that the model behaved as predicted by theory. 34 figs., 52 refs., 5 tabs

A Versatile System for the In-Field Non-Destructive Characterization of Radioactive Waste Packages and of Objects in the Defense against Nuclear Threats

International Nuclear Information System (INIS)

Buecherl, T.; Gostomski, Ch.-Lierse-von

2013-06-01

In-filed non-destructive characterization of radioactive waste packages and of objects in the defense of nuclear threats requires purpose-built but similar equipment. Based on commercially available measuring devices like dose-rate and gamma detectors, X-ray and gamma-transmission sources etc. a versatile and mobile mechanical positioning system for these devices is designed, assembled and operated facilitating basic to even complex measuring procedures. Several in-field measuring campaigns resulted in its further optimization. Today an highly mobile and flexible mechanical manipulator system is available supporting nearly all types of required measuring devices thus rising to nearly all occasions. (authors)

Monoclonal antibody-based dipstick assay: a reliable field applicable technique for diagnosis of Schistosoma mansoni infection using human serum and urine samples.

Science.gov (United States)

Demerdash, Zeinab; Mohamed, Salwa; Hendawy, Mohamed; Rabia, Ibrahim; Attia, Mohy; Shaker, Zeinab; Diab, Tarek M

2013-02-01

A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.

A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay.

Science.gov (United States)

Babu, Binoy; Washburn, Brian K; Ertek, Tülin Sarigül; Miller, Steven H; Riddle, Charles B; Knox, Gary W; Ochoa-Corona, Francisco M; Olson, Jennifer; Katırcıoğlu, Yakup Zekai; Paret, Mathews L

2017-09-01

Rose rosette disease, caused by Rose rosette virus (RRV; genus Emaravirus) is a major threat to the rose industry in the U.S. The only strategy currently available for disease management is early detection and eradication of the infected plants, thereby limiting its potential spread. Current RT-PCR based diagnostic methods for RRV are time consuming and are inconsistent in detecting the virus from symptomatic plants. Real-time RT-qPCR assay is highly sensitive for detection of RRV, but it is expensive and requires well-equipped laboratories. Both the RT-PCR and RT-qPCR cannot be used in a field-based testing for RRV. Hence a novel probe based, isothermal reverse transcription-recombinase polymerase amplification (RT-exoRPA) assay, using primer/probe designed based on the nucleocapsid gene of the RRV has been developed. The assay is highly specific and did not give a positive reaction to other viruses infecting roses belonging to both inclusive and exclusive genus. Dilution assays using the in vitro transcript showed that the primer/probe set is highly sensitive, with a detection limit of 1 fg/μl. In addition, a rapid technique for the extraction of viral RNA (rose varieties, collected from different states in the U.S. The entire process, including the extraction can be completed in 25min, with less sophisticated equipments. The developed assay can be used with high efficiency in large scale field testing for rapid detection of RRV in commercial nurseries and landscapes. Copyright © 2017 Elsevier B.V. All rights reserved.

Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay.

Science.gov (United States)

Tsuchiya, Akira; Asai, Daisuke; Kang, Jeong-Hun; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki

2012-02-15

To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays. Copyright © 2011 Elsevier Inc. All rights reserved.

Development of an automatic smear sampler and a polymer film for surface radioactive contamination assay

International Nuclear Information System (INIS)

Seo, B.-K.; Lee, K.-W.; Woo, Z.-H.; Jeong, K.-S.; Oh, W.-Z.; Han, M.-J.

2004-01-01

Measurement of the surface contamination by an indirect method is subject to the various kinds of error according to the sampling person and needs much time and effort in the sampling and assay. In this research, an automatic smear sampler is developed. It improved efficiency for assay work of surface contamination level achieved periodically in a radiation controlled area. Using an automatic smear sampler developed, it is confirmed that radioactive contaminated materials are uniformly transferred to smear paper more than any sampling method by an operator. Also, Solid scintillation proximity membranes were prepared for measuring the amount of radioactive contamination in laboratories contaminated by the low energy beta-ray emitter, such as 3 H and 14 C. Polysulfone scintillation proximity membranes were prepared by impregnating Cerium Activated Yttrium Silicate (CAYS), an inorganic fluor, in a membrane structure. The inorganic fluor-impregnated membranes were applied to detect the radioactive surface contamination. The preparation of membranes was divided into two processes. A supporting polymer film was made of casting solutions consisting of polysulfone and solvent, their cast film being solidified by vacuum evaporation. CAYS-dispersed polymer solutions were cast over the first, solidified polymer films and coagulated either by evaporating solvent in the solution with non-solvent in a coagulation bath. The prepared membranes had two distinguished, but tightly attached, double layers : one is the supporting layer of dense polymer film and the other results revealed that the prepared membranes were efficient to monitor radioactive contamination with reliable counting ability. For enhancement of pick-up and measurement efficiency, the membrane was prepared with the condition of different membrane solidification. The scintillation produced by interaction with radiation and CAYS was measured with photomultiplier tube. The test results showed that the prepared

Influence of non-radioactive payload parameters on radioactive shipping packages

International Nuclear Information System (INIS)

Drez, P.E.; Murthy, D.V.S.; Temus, C.J.; Quinn, G.J.; Ozaki, C.

1989-01-01

The transport of radioactive waste materials in radioactive material (RAM) packages involves two components: the packaging used for transportation, and the waste which forms the payload. The payload is usually comprised of non-radioactive materials contaminated with radionuclides. The non-radionuclide payload characteristics can often be a controlling factor in determining the restrictions imposed on the certification of the package. This paper describes these package/payload interactions and the limiting parameters for the Transuranic Package Transporter-II (TRUPACT-II), designed for the transportation of Contact Handled Transuranic (CH-TRU) waste. The parameters discussed include the physical and chemical form of the payload, the configuration of the waste, and resulting gas generation and gas release phenomena. Brief descriptions of the TRUPACT-II package and its payload are presented initially

Survey of EEC solid waste arisings and performance of non-destructive assay systems

International Nuclear Information System (INIS)

Bremner, W.B.; Adaway, D.W.; Yates, A.

1992-01-01

This report covers the work carried out during an one-year contract which surveyed the radioactive solid waste arisings in EEC Member States and also tabulated information on the performance of the non-destructive assay (NDA) system used. The work was jointly carried out with CEA partners at Cadarache and Paris. The tabulated data give information on types, packaging, associated activity, and NDA capability of the utilities or research organisations. Some short comings in NDA capabilities are identified and possible solutions are given

Potential development of non-destructive assay for nuclear safeguards

International Nuclear Information System (INIS)

Benoit, R.; Cuypers, M.; Guardini, S.

1983-01-01

After a brief summary on the role of non-destructive assay in safeguarding the nuclear fuel cycle, its evolution from NDA methods development to other areas is illustrated. These areas are essentially: a) the evaluation of the performances of NDA techniques in field conditions; b) introduction of full automation of measurement instrument operation, using interactive microprocessors and of measurement data handling evaluation and retrieval features; c) introduction of the adequate link and compatibility to assure NDA measurement data transfer in an integrated safeguards data evaluation scheme. In this field, the Joint Research Centre (JRC) of the Commission of the European Communities (CEC) is developing and implementing a number of techniques and methodologies allowing an integrated and rational treatment of the large amount of safeguards data produced. In particular for the non-destructive assay measurements and techniques, the JRC has studied and tested methodologies for the automatic generation and validation of data of inventory verification. In order to apply these techniques successfully in field, the JRC has studied the design requirements of NDA data management and evaluation systems. This paper also discusses the functional requirements of an integrated system for NDA safeguards data evaluation

Radioactive and non-radioactive polychlorinated biphenyl (PCB) management at Hanford

International Nuclear Information System (INIS)

Leonard, W.W.; Gretzinger, R.F.; Cox, G.R.

1986-01-01

Conformance to all state and federal regulations is the goal of Rockwell in the management of both radioactive and non-radioactive PCB's at Hanford. A continuing effort is being made to locate, remove, and properly dispose of all PCB's. As improved methods of management are developed, consideration will be given to them for their adaptation into the Hanford Site PCB Management Plan

Comparing differential tolerance of native and non-indigenous marine species to metal pollution using novel assay techniques

International Nuclear Information System (INIS)

Piola, Richard F.; Johnston, Emma L.

2009-01-01

Recent research suggests anthropogenic disturbance may disproportionately advantage non-indigenous species (NIS), aiding their establishment within impacted environments. This study used novel laboratory- and field-based toxicity testing to determine whether non-indigenous and native bryozoans (common within marine epibenthic communities worldwide) displayed differential tolerance to the common marine pollutant copper (Cu). In laboratory assays on adult colonies, NIS showed remarkable tolerance to Cu, with strong post-exposure recovery and growth. In contrast, native species displayed negative growth and reduced feeding efficiency across most exposure levels. Field transplant experiments supported laboratory findings, with NIS growing faster under Cu conditions. In field-based larval assays, NIS showed strong recruitment and growth in the presence of Cu relative to the native species. We suggest that strong selective pressures exerted by the toxic antifouling paints used on transport vectors (vessels), combined with metal contamination in estuarine environments, may result in metal tolerant NIS advantaged by anthropogenically modified selection regimes. - Greater tolerance to pollutants in marine NIS may increase the risk of invasion in port and harbours worldwide by providing a competitive advantage over native taxa.

Non-destructive assay of leached hulls in a nuclear fuel reprocessing plant

International Nuclear Information System (INIS)

Hofstetter, K.J.; Henderson, B.C.; Gray, J.H.; Huff, G.A.

1978-01-01

The hull monitor at the Barnwell Nuclear Fuels Plant (BNFP) will be a remotely controlled, fully automated system designed to quantitatively assay leached hulls for undissolved U and Pu. The hull monitor will assay the hulls from one metric ton of fuel per dissolver basket with the design goal of detecting 0.1% undissolved fuel and yet remain within the framework of the BNFP materials flow of five hull baskets per day. The non-destructive assay will be accomplished using a computer-based gamma-ray pulse height analysis system employing a 5 x 5 inch NaI(Tl) scintillation detector. The intense radiations from the fission product isotopes and the activation product isotopes produced in the reactor prevent direct assay of the undissolved fuel left in the hulls. The measurement will be made indirectly by demonstrating a correlation between the amount of 144 Ce undissolved and the remaining U. The isotope 144 Ce is a direct fission product with high cumulative yield. The daughter isotope 144 Pr has a gamma ray at 2.18 MeV well above other predominant radiations in the spectrum from the major interferences 60 Co, 58 Co, 95 Zr( 95 Nb), 137 Cs and 106 Ru( 106 Rh). Segmented scanning operation of the hull monitor is accomplished by rotation and vertical transversal of the hulls container past the detector station. Proper collimation and absorbers are required to maximize the 144 Ce( 144 Pr) to background ratio. A basket indexer is provided which monitors the scanning rate and ensures repositioning. The leached hull monitor system will be interfaced to a computer-based multichannel analyzer for ease of operation and data handling. A calibration basket has been fabricated to accomodate radioactive sources and inactive Zircaloy hulls

Ethanol production from rice on radioactively contaminated field toward sustainable rice farming

International Nuclear Information System (INIS)

Yokoyama, Shinya; Izumi, Bintaro; Oki, Kazuo

2011-01-01

Radioactive species such as 137 Cs were discharged from Fukushima Daiichi Nuclear Power Plant which was severely damaged by the enormous earthquake and tsunami. Cropland has been radioactively contaminated by 137 Cs etc. and it seems impossible to plant rice due to the non-suitability for food. According to the reports, 137 Cs transferred into the rice from soil is less than 1% on the average. Therefore, it is expected that the concentration of 137 Cs in bioethanol will be well below the tentative restriction value even if bioethanol could be produced from the rice. It is proposed that the rice field should be filled with water to avoid the flow of runoff contaminated by radioactive cesium compounds because they are insoluble in aqueous phase and that bioethanol should be produced from the rice in order to maintain the multifunction of rice field and to continue the agriculture. If rice farming is halted and neglected, agricultural function of rice field as well as local community will be permanently destroyed. (author)

Experience with local lymph node assay performance standards using standard radioactivity and nonradioactive cell count measurements.

Science.gov (United States)

Basketter, David; Kolle, Susanne N; Schrage, Arnhild; Honarvar, Naveed; Gamer, Armin O; van Ravenzwaay, Bennard; Landsiedel, Robert

2012-08-01

The local lymph node assay (LLNA) is the preferred test for identification of skin-sensitizing substances by measuring radioactive thymidine incorporation into the lymph node. To facilitate acceptance of nonradioactive variants, validation authorities have published harmonized minimum performance standards (PS) that the alternative endpoint assay must meet. In the present work, these standards were applied to a variant of the LLNA based on lymph node cell counts (LNCC) run in parallel as a control with the standard LLNA with radioactivity measurements, with threshold concentrations (EC3) being determined for the sensitizers. Of the 22 PS chemicals tested in this study, 21 yielded the same results from standard radioactivity and cell count measurements; only 2-mercaptobenzothiazole was positive by LLNA but negative by LNCC. Of the 16 PS positives, 15 were positive by LLNA and 14 by LNCC; methylmethacrylate was not identified as sensitizer by either of the measurements. Two of the six PS negatives tested negative in our study by both LLNA and LNCC. Of the four PS negatives which were positive in our study, chlorobenzene and methyl salicylate were tested at higher concentrations than the published PS, whereas the corresponding concentrations resulted in consistent negative results. Methylmethacrylate and nickel chloride tested positive within the concentration range used for the published PS. The results indicate cell counts and radioactive measurements are in good accordance within the same LLNA using the 22 PS test substances. Comparisons with the published PS results may, however, require balanced analysis rather than a simple checklist approach. Copyright © 2011 John Wiley & Sons, Ltd.

The local lymph node assay being too sensitive?

Science.gov (United States)

Hans-Werner, Vohr; Jürgen, Ahr Hans

2005-12-01

The local lymph node assay (LLNA) and modifications thereof were recently recognized by the OECD as stand-alone methods for the detection of skin-sensitizing potential. However, although the validity of the LLNA was acknowledged by the ICCVAM, attention was drawn to one major problem, i.e., the possibility of false positive results caused by non-specific cell activation as a result of inflammatory processes in the skin (irritation). This is based on the fact that inflammatory processes in the skin may lead to non-specific activation of dendritic cells, cell migration and non-specific proliferation of lymph node cells. Measuring cell proliferation by radioactive or non-radioactive methods, without taking the irritating properties of test items into account, leads thus to false positive reactions. In this paper, we have compared both endpoints: (1) cell proliferation alone and (2) cell proliferation in combination with inflammatory (irritating) processes. It turned out that a considerable number of tests were "false positive" to the definition mentioned above. By excluding such false positive results the LLNA seems not to be more sensitive than relevant guinea pig assays. These various methods and results are described here.

Non-radioactive waste management in a Nuclear Energy Research Institution

Energy Technology Data Exchange (ETDEWEB)

Furusawa, Helio A.; Martins, Elaine A.J.; Cotrim, Marycel E.B.; Pires, Maria A. F., E-mail: helioaf@ipen.br, E-mail: elaine@ipen.br, E-mail: mecotrim@ipen.br, E-mail: mapires@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEM-SP), Sao Paulo, SP (Brazil). Centro de Quimica e Meio Ambiente

2013-07-01

For more than 50 years, non-radioactive materials have been used in processes at IPEN to support the nuclear fuel development and all related activities. Reagents, raw materials, products and by-products have been stored. Many of these are hazardous highly toxic or reactants materials. Some years ago actions sent part of these non-radioactive waste materials to proper disposal (technical incineration) resulting in an Institutional Non-Radioactive Waste Management Program. In 2005, an internal set of procedures and information entitled - Guia de Procedimentos para Armazenamento, Tratamento e Descarte de Residuos de Laboratorio Quimico - (Guide of Procedures for Storage, Treatment, and Disposal of Chemistry Laboratory Wastes) - was published to be used at the IPEN's facilities. A data base managed by software was created in order to allow the Units to input data and information about the routinely generated wastes and those already existing. Even after disposing so huge amount of wastes, a latent demand still exists. Several goals were achieved notably a well-organized and roomy space; safer storage places; local, state, and nationwide laws enforcement (for radioactive and non-radioactive materials); and improvement in chemicals control as hazardous and aged materials are more frequently disposed. A special stress was conducted to know and follow laws, regulations, and technical norms as the entire process is very detailed and this is not a day-by-day routine for the IPEN's technical personnel. The immediate consequence is that the safer the workplace the safer the nuclear related activities are done. (author)

Non-radioactive waste management in a Nuclear Energy Research Institution

International Nuclear Information System (INIS)

Furusawa, Helio A.; Martins, Elaine A.J.; Cotrim, Marycel E.B.; Pires, Maria A. F.

2013-01-01

For more than 50 years, non-radioactive materials have been used in processes at IPEN to support the nuclear fuel development and all related activities. Reagents, raw materials, products and by-products have been stored. Many of these are hazardous highly toxic or reactants materials. Some years ago actions sent part of these non-radioactive waste materials to proper disposal (technical incineration) resulting in an Institutional Non-Radioactive Waste Management Program. In 2005, an internal set of procedures and information entitled - Guia de Procedimentos para Armazenamento, Tratamento e Descarte de Residuos de Laboratorio Quimico - (Guide of Procedures for Storage, Treatment, and Disposal of Chemistry Laboratory Wastes) - was published to be used at the IPEN's facilities. A data base managed by software was created in order to allow the Units to input data and information about the routinely generated wastes and those already existing. Even after disposing so huge amount of wastes, a latent demand still exists. Several goals were achieved notably a well-organized and roomy space; safer storage places; local, state, and nationwide laws enforcement (for radioactive and non-radioactive materials); and improvement in chemicals control as hazardous and aged materials are more frequently disposed. A special stress was conducted to know and follow laws, regulations, and technical norms as the entire process is very detailed and this is not a day-by-day routine for the IPEN's technical personnel. The immediate consequence is that the safer the workplace the safer the nuclear related activities are done. (author)

Use of a Gantry robot in the assay of radioactive materials

International Nuclear Information System (INIS)

Phelan, P.F.; Beugelsdijk, T.J.; Powell, W.D.; Schneider, D.N.; Staley, H.C.; Blankenship, R.

1989-01-01

A large industrial robot has been installed in our plutonium processing facility to speed the assay of materials in process. The robot routinely transports radioactive items weighing 20 lbs or more between an automated inventory system and the analytical instruments. Because the system operates unattended under computer control, assays are now performed around-the-clock instead of just 8 hrs per day, thereby greatly increasing the utilization of our instruments. (When the system becomes fully operational, we except a four-fold increase in productivity.) In addition, recordkeeping has improved, the plutonium is better protected from theft, and our personnel are exposed to less radiation than before. 4 figs

Non-destructive nuclear forensics of radioactive samples

Energy Technology Data Exchange (ETDEWEB)

Rogge, R.B. [Canadian Neutron Beam Centre, Chalk River, ON (Canada); Alexander, Q.; Bentoumi, G.; Dimayuga, F. [Atomic Energy of Canada Limited, Chalk River, ON (Canada); Flacau, R. [Canadian Neutron Beam Centre, Chalk River, ON (Canada); Li, G.; Li, L.; Sur, B. [Atomic Energy of Canada Limited, Chalk River, ON (Canada)

2014-07-01

It is a matter of public safety and security to be able to examine suspicious packages of unknown origin. If the package is radioactive and sealed (i.e., the radioactive materials contained in the package, including their chemical and physical forms, are unknown), there is a significant risk on how to handle the package and eventually safely dispose of its contents. Within the context of nuclear security, nuclear forensics helps address the key issue of identifying the nature and origin of radioactive and nuclear material in order to improve physical protection measures and prevent future theft or diversion of these materials. Nuclear forensics utilizes analytical techniques, destructive and non-destructive, developed for applications related to nuclear fuel cycles. This paper demonstrates the non-destructive examination techniques that can be used to inspect encapsulated radioactive samples. Results of γ spectroscopy, X-ray spectroscopy, neutron imaging, neutron diffraction, and delayed neutron analysis as applied to an examination of sealed capsules containing unknown radioactive materials are presented. The paper also highlights the value of these techniques to the overall nuclear forensic investigation to determine the origin of these unknown radioactive materials. (author)

Effects of non-radioactive material around radioactive material on PET image quality

International Nuclear Information System (INIS)

Toshimitsu, Shinya; Yamane, Azusa; Hirokawa, Yutaka; Kangai, Yoshiharu

2015-01-01

Subcutaneous fat is a non-radioactive material surrounding the radioactive material. We developed a phantom, and examined the effect of subcutaneous fat on PET image quality. We created a cylindrical non-radioactive mimic of subcutaneous fat, placed it around a cylindrical phantom in up to three layers with each layer having a thickness of 20 mm to reproduce the obesity caused by subcutaneous fat. In the cylindrical phantom, hot spheres and cold spheres were arranged. The radioactivity concentration ratio between the hot spheres and B.G. was 4:1. The radioactivity concentration of B.G. was changed as follows : 1.33, 2.65, 4.00, and 5.30 kBq/mL. 3D-PET image were collected during 10 minutes. When the thickness of the mimicked subcutaneous fat increased from 0 mm to 60 mm, noise equivalent count decreased by 58.9-60.9% at each radioactivity concentration. On the other hand, the percentage of background variability increased 2.2-5.2 times. Mimic subcutaneous fat did not decrease the percentage contrast of the hot spheres, and did not affect the cold spheres. Subcutaneous fat decreases the noise equivalent count and increases the percentage of background variability, which degrades PET image quality. (author)

Ontario hydro radioactive material transportation field guide

International Nuclear Information System (INIS)

Howe, W.

1987-01-01

The recent introduction of both the AECB Transport Packaging of Radioactive Material Regulations and Transport Canada's Transportation of Dangerous Goods Regulations have significantly altered the requirements for transporting radioactive material in Canada. Extensive additional training as well as certification of several hundred Ontario Hydro employees has been necessary to ensure compliance with the additional and revised regulatory requirements. To assist in the training of personnel, an 'active' corporate Ontario Hydro Field Guide for Radioactive Material Transport document has been developed and published. The contents of this Field Guide identify current Ontario Hydro equipment and procedures as well as the updated relevant regulatory requirements within Canada. In addition, to satisfying Ontario Hydro requirements for this type of information over two thousand of these Field Guides have been provided to key emergency response personnel throughout the province of Ontario to assist in their transportation accident response training

A high-performance liquid chromatography method for the serotonin release assay is equivalent to the radioactive method.

Science.gov (United States)

Sono-Koree, N K; Crist, R A; Frank, E L; Rodgers, G M; Smock, K J

2016-02-01

The serotonin release assay (SRA) is considered the gold standard laboratory test for heparin-induced thrombocytopenia (HIT). The historic SRA method uses platelets loaded with radiolabeled serotonin to evaluate platelet activation by HIT immune complexes. However, a nonradioactive method is desirable. We report the performance characteristics of a high-performance liquid chromatography (HPLC) SRA method. We validated the performance characteristics of an HPLC-SRA method, including correlation with a reference laboratory using the radioactive method. Serotonin released from reagent platelets was quantified by HPLC using fluorescent detection. Results were expressed as % release and classified as positive, negative, or indeterminate based on previously published cutoffs. Serum samples from 250 subjects with suspected HIT were tested in the HPLC-SRA and with the radioactive method. Concordant classifications were observed in 230 samples (92%). Sera from 41 healthy individuals tested negative. Between-run imprecision studies showed standard deviation of performance characteristics, equivalent to the historic radioactive method, but avoids the complexities of working with radioactivity. © 2015 John Wiley & Sons Ltd.

Natural radioactivity at Podravina gas fields

International Nuclear Information System (INIS)

Kovac, J.; Marovic, G.

2006-01-01

In Croatia, natural gas is an important source of energy, where its use exceeds other sources by one third. Composed primarily of the methane, natural gas from Croatian Podravina gas fields, beside other impurities, contains small amounts of radioactive elements. At Gas Treatment Plant (GTP) Molve, technological procedures for purification of natural gas and its distribution are performed. With yearly natural gas production of 3.5 109 m3 GTP Molve is major Croatian energy resource. Its safety and environment impact is matter of concern. Using different radioactivity measuring techniques the exposure of population to ionizing radiation were calculated at Central Natural Gas Station Molve and the underground wells. The measurement techniques included in-situ gamma spectrometric measurements, from which contribution to absorbed dose of the natural radionuclide in soil were calculated. Exposure dose measurements were performed using T.L.-dosimeters, and L.A.R.A. electronic dosimeters as well as field dose rate meter. Comparing used different radioactivity measuring methods, the correlations have been calculated. (authors)

Further experience with the local lymph node assay using standard radioactive and nonradioactive cell count measurements.

Science.gov (United States)

Kolle, Susanne N; Basketter, David; Schrage, Arnhild; Gamer, Armin O; van Ravenzwaay, Bennard; Landsiedel, Robert

2012-08-01

In a previous study, the predictive capacity of a modified local lymph node assay (LLNA) based on cell counts, the LNCC, was demonstrated to be closely similar to that of the original assay. In addition, a range of substances, including some technical/commercial materials and a range of agrochemical formulations (n = 180) have also been assessed in both methods in parallel. The results in the LNCC and LLNA were generally consistent, with 86% yielding an identical classification outcome. Discordant results were associated with borderline data and were evenly distributed between the two methods. Potency information derived from each method also demonstrated good consistency (n = 101), with 93% of predictions being close. Skin irritation was observed only infrequently and was most commonly associated with positive results; it was not associated with the discordant results. Where different vehicles were used with the same test material, the effect on sensitizing activity was modest, consistent with historical data. Analysis of positive control data indicated that the LNCC and LLNA displayed similar levels of biological variation. When taken in combination with the previously published results on LLNA Performance Standard chemicals, it is concluded that the LNCC provides a viable non-radioactive alternative to the LLNA for the assessment of substances, including potency predictions, as well as for the evaluation of preparations. Copyright © 2012 John Wiley & Sons, Ltd.

Investigation of radioactive contamination at non-radioactive drains of the Tsuruga Nuclear Power Station

International Nuclear Information System (INIS)

Koide, Hiroaki; Imanaka, Tetsuji; Ebisawa, Toru; Kawano, Shinji; Kobayashi, Keiji.

1982-05-01

In April, 1981, it was disclosed that a drainage area at the Tsuruga Nuclear Power Station was so much contaminated with radioactivites. Although Ministry of International Trade and Industry (MITI) officially provided an explanation of a process that resulted in the contamination, many problems remain unsolved on account of insufficient and limited investigations. The authors collected mud samples from contaminated manholes and examined radioactivities in them through the measurement of #betta#- and #betta#-spectra. Chemical separation of the samples was carried out in order to obtain precise concentration of radioactive cesium. Results are as follows: i) the concentration of radioactivities does not show monotonous decrease along the stream line but an anomalous peak at downstream manholes, ii) at the manhole specified No. 6 located rather downstream, 137 Cs concentration is significantly high and the composition of radioactive nuclides is quite different from that in the other manholes, and iii) additional radioactive contamination was observed in other manholes of non-radioactive drains which would not be influenced by the accident explained by MITI. Our present work has provided much more data than by MITI and made it clear that the overall data cnnot be consistent with the simple MITI explanation; a single radioactive release accident caused the disclosed contamination. It is concluded that non-radioactive water drains at the Tsuruga Nuclear Power Station had been under continual contamination. (author)

A flow cytometry-based assay for quantifying non-plaque forming strains of yellow fever virus.

Directory of Open Access Journals (Sweden)

Erika Hammarlund

Full Text Available Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE on cell monolayers. In our hands, the Dakar strain of yellow fever virus (YFV-Dakar could not be measured by plaque assay (PA, focus-forming assay (FFA, or by measurement of CPE. For these reasons, we developed a YFV-specific monoclonal antibody (3A8.B6 and used it to optimize a highly sensitive flow cytometry-based tissue culture limiting dilution assay (TC-LDA to measure levels of infectious virus. The TC-LDA was performed by incubating serial dilutions of virus in replicate wells of C6/36 cells and stained intracellularly for virus with MAb 3A8.B6. Using this approach, we could reproducibly quantitate YFV-Dakar in tissue culture supernatants as well as from the serum of viremic rhesus macaques experimentally infected with YFV-Dakar. Moreover, the TC-LDA approach was >10-fold more sensitive than standard plaque assay for quantitating typical plaque-forming strains of YFV including YFV-17D and YFV-FNV (French neurotropic vaccine. Together, these results indicate that the TC-LDA technique is effective for quantitating both plaque-forming and non-plaque-forming strains of yellow fever virus, and this methodology may be readily adapted for the study and quantitation of other non-plaque-forming viruses.

Management of radioactive waste from non-power applications in the Netherlands

International Nuclear Information System (INIS)

Codee, H.D.K.

2002-01-01

Radioactive waste results from the use of radioactive materials in hospitals, research establishments, industry and nuclear power plants. The Netherlands forms a good example of a country with a small and in the near future ending nuclear power programme. The radioactive waste from non-power applications therefore strongly influences the management choices. A dedicated waste management company COVRA, the Central Organisation for Radioactive Waste manages all radioactive waste produced in the Netherlands. For the small volume, but broad spectrum of radioactive waste, a management system was developed based on the principle to isolate, to control and to monitor the waste. Long-term storage is an important element in this management strategy. It is not seen as a 'wait and see' option but as a necessary step in the strategy that will ultimately result in final removal of the waste. Since the waste will remain retrievable for a long time new technologies and new disposal options can be applied when available and feasible. (author)

Can the same principles be used for the management of radioactive and non-radioactive waste?

International Nuclear Information System (INIS)

Bengtsson, Gunnar.

1989-01-01

Non-radioactive waste has a much more complex composition than radioactive waste and appears in much larger quantities. The two types of waste have, however, some properties in common when it comes to their longterm impact on health and the environment. The occurrence in both of substances that may exist for generations and may cause cancer provides one example. Both types of waste also always occur together. It is therefore proposed that the same basic principles could be applied for the management of radioactive and non-radioactive waste. By doing so one may increase the efficiency of policy development, research and practical management. This is particurlarly importand for the very costly restoration of old disposal sites which have earlier been poorly managed. (author)

Neutronic measurements of radioactive waste; Les mesures neutroniques des dechets radioactifs

Energy Technology Data Exchange (ETDEWEB)

Perot, B

1997-12-31

This document presents the general matters involved in the radioactive waste management and the different non destructive assays of radioactivity. The neutronic measurements used in the characterization of waste drums containing emitters are described with more details, especially the active neutronic interrogation assays with prompt or delayed neutron detection: physical principle, signal processing and evaluation of the detection limit. (author).

Differentiation of drug and non-drug Cannabis using a single nucleotide polymorphism (SNP) assay.

Science.gov (United States)

Rotherham, D; Harbison, S A

2011-04-15

Cannabis sativa is both an illegal drug and a legitimate crop. The differentiation of illegal drug Cannabis from non-drug forms of Cannabis is relevant in the context of the growth of fibre and seed oil varieties of Cannabis for commercial purposes. This differentiation is currently determined based on the levels of tetrahydrocannabinol (THC) in adult plants. DNA based methods have the potential to assay Cannabis material unsuitable for analysis using conventional means including seeds, pollen and severely degraded material. The purpose of this research was to develop a single nucleotide polymorphism (SNP) assay for the differentiation of "drug" and "non-drug"Cannabis plants. An assay was developed based on four polymorphisms within a 399 bp fragment of the tetrahydrocannabinolic acid (THCA) synthase gene, utilising the snapshot multiplex kit. This SNP assay was tested on 94 Cannabis plants, which included 10 blind samples, and was able to differentiate between "drug" and "non-drug"Cannabis in all cases, while also differentiating between Cannabis and other species. Non-drug plants were found to be homozygous at the four sites assayed while drug Cannabis plants were either homozygous or heterozygous. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

ASTRO Research Fellow Presentation - A comparison of the comet assay and pulsed-field gel electrophoresis as a predictive assay for radiosensitivity in human fibroblasts

International Nuclear Information System (INIS)

Sarkaria, Jann N.; Eady, John J.; Peacock, John H.; Steel, G. Gordon

1996-01-01

Purpose/Objective: To determine whether neutral lysis single-cell gel electrophoresis (comet assay) or pulsed-field gel electrophoresis (PFGE) can be used as a predictive assay for tissue response to radiotherapy as an alternative to clonogenic survival measurements. Materials and Methods: The comet assay has been widely used to measure DNA double strand breaks (dsb) in individual cells, and it has been suggested that it could be used as an alternative to clonogenic assays to measure radiosensitivity. Previous studies in this lab have demonstrated the ability of pulsed-field gel electrophoresis, which also measures DNA dsb, to accurately predict the radiosensitivity of a panel of fibroblasts based on determination of residual DNA dsb. As part of an ongoing study examining the relationship between fibroblast radiosensitivity and normal-tissue radiation reactions, we have compared the sensitivity and accuracy of the comet assay and PFGE on a different panel of non-transformed fibroblasts derived from breast cancer patients who developed severe radiation late effects and from case-matched controls. For the measurement of initial damage, cells were suspended in PBS and irradiated on ice for the comet assay and irradiated in agarose plugs on ice for pFGE. Residual damage was measured following irradiation of confluent cultures at 37 degree sign C and subsequent incubation for four hours prior to preparation of agarose slides and plugs. All irradiations were performed with a 59 TBq 60 Co source at a dose rate of 1.7 Gy/min. Electrophoresis was performed following neutral pH cell lysis. Comet images were captured and analyzed using Optimas software with DNA damage quantitated by the comet moment. PFGE gels were analyzed using a phosphor-image analysis system and damage was quantitated based on the percent of activity released from the well. Results: The comet assay was able to detect initial DNA damage at a threshold of 5 Gy and exhibited a linear dose

Separation of non-hazardous, non-radioactive components from ICPP calcine via chlorination

International Nuclear Information System (INIS)

Nelson, L.O.

1995-05-01

A pyrochemical treatment method for separating non-radioactive from radioactive components in solid granular waste accumulated at the Idaho Chemical Processing Plant was investigated. The goal of this study was to obtain kinetic and chemical separation data on the reaction products of the chlorination of the solid waste, known as calcine. Thermodynamic equilibrium calculations were completed to verify that a separation of radioactive and non-radioactive calcine components was possible. Bench-scale chlorination experiments were completed subsequently in a variety of reactor configurations including: a fixed-bed reactor (reactive gases flowed around and not through the particle bed), a packed/fluidized-bed reactor, and a packed-bed reactor (reactive gases flowed through the particle bed). Chemical analysis of the reaction products generated during the chlorination experiments verified the predictions made by the equilibrium calculations. An empirical first-order kinetic rate expression was developed for each of the reactor configurations. 20 refs., 16 figs., 21 tabs

Proposal of threshold levels for the definition of non-radioactive wastes

International Nuclear Information System (INIS)

Yoshida, Yoshikazu

1979-01-01

With increasing amounts of radioactive wastes along with the advances of nuclear power generation and radioactive material utilizations, the needs for management cost reduction and resource saving have arisen. Under the situation, the threshold levels for the definition of non-radioactive solid wastes are required. The problem has been studied by an ad hoc committee in Nuclear Safety Research Association, by the request of the Science and Technology Agency. The matters described are the procedures of deriving the threshold levels, the feasibility studies of the management of waste threshold-level with several enterprises, and future subjects of study. The threshold levels are grouped in two, i.e. the unconditional level and the conditional level. According to the unconditional threshold level, solid wastes are separated definitely into radioactive and non-radioactive ones. According to the conditional threshold level, under certain conditions, some radioactive solid wastes according to the unconditional level are regarded as non-radioactive ones. (J.P.N.)

Allergenicity evaluation of p-chloro-m-cresol and p-chloro-m-xylenol by non-radioactive murine local lymph-node assay and multiple-dose guinea pig maximization test

International Nuclear Information System (INIS)

Yamano, Tetsuo; Shimizu, Mitsuru; Noda, Tsutomu

2003-01-01

p-Chloro-m-cresol (PCMC) and p-chloro-m-xylenol (PCMX) are known to cause allergic contact dermatitis. For risk assessment of skin sensitizers, information on dose-response profiles in the induction and elicitation phases and cross-reactivity with analogous chemicals are important. In the non-radioactive local lymph-node assay (LLNA) using 5-bromo-2'-deoxyuridine instead of 3 H-methyl thymidine, significant effect on lymph node cell proliferation was detected at 10% PCMC and 25% PCMX, while in the multiple-dose guinea pig maximization test (GPMT) at least one animal tested in the group was sensitized at a 5 ppm induction dose of either chemical. When mean skin reaction score in an animal group maximally sensitized with each allergen with the GPMT was plotted against log challenge concentration, linear regression lines with high correlations were obtained in both cases. The calculated elicitation threshold was lower for PCMC than PCMX. The area under the linear regression line between the threshold point and 1% of the elicitation concentration, another index of relative elicitation potency, was also greater for PCMC. Bidirectional cross-reactivity between PCMX and PCMC was detected in the GPMT. PCMC was thus identified in both LLNA and GPMT as a stronger sensitizer than PCMX in both the induction and elicitation phases. These results suggest that the non-radioactive LLNA is a simple and useful method for evaluating allergenicity in the induction phase, while the GPMT using a maximally sensitized animal group is more suitable for assessing the dose-response profile and cross-reactivity in the elicitation phase

Selection of non-destructive assay methods: Neutron counting or calorimetric assay?

International Nuclear Information System (INIS)

Cremers, T.L.; Wachter, J.R.

1994-01-01

The transition of DOE facilities from production to D ampersand D has lead to more measurements of product, waste, scrap, and other less attractive materials. Some of these materials are difficult to analyze by either neutron counting or calorimetric assay. To determine the most efficacious analysis method, variety of materials, impure salts and hydrofluorination residues have been assayed by both calorimetric assay and neutron counting. New data will be presented together with a review of published data. The precision and accuracy of these measurements are compared to chemistry values and are reported. The contribution of the gamma ray isotopic determination measurement to the overall error of the calorimetric assay or neutron assay is examined and discussed. Other factors affecting selection of the most appropriate non-destructive assay method are listed and considered

Significantly improving nuclear resonance fluorescence non-destructive assay by using the integral resonance transmission method and photofission

International Nuclear Information System (INIS)

Angell, Christopher T.; Hayakawa, Takehito; Shizuma, Toshiyuki; Hajima, Ryoichi

2013-01-01

Non-destructive assay (NDA) of 239 Pu in spent nuclear fuel or melted fuel using a γ-ray beam is possible using self absorption and the integral resonance transmission method. The method uses nuclear resonance absorption where resonances in 239 Pu remove photons from the beam, and the selective absorption is detected by measuring the decrease in scattering in a witness target placed in the beam after the fuel, consisting of the isotope of interest, namely 239 Pu. The method is isotope specific, and can use photofission or scattered γ-rays to assay the 239 Pu. It overcomes several problems related to NDA of melted fuel, including the radioactivity of the fuel, and the unknown composition and geometry. This talk will explain the general method, and how photofission can be used to assay specific isotopes, and present example calculations. (author)

A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus.

Science.gov (United States)

Decaro, Nicola; Elia, Gabriella; Desario, Costantina; Roperto, Sante; Martella, Vito; Campolo, Marco; Lorusso, Alessio; Cavalli, Alessandra; Buonavoglia, Canio

2006-09-01

A minor groove binder (MGB) probe assay was developed to discriminate between type 2-based vaccines and field strains of canine parvovirus (CPV). Considering that most of the CPV vaccines contain the old type 2, no longer circulating in canine population, two MGB probes specific for CPV-2 and the antigenic variants (types 2a, 2b and 2c), respectively, were labeled with different fluorophores. The MGB probe assay was able to discriminate correctly between the old type and the variants, with a detection limit of 10(1) DNA copies and a good reproducibility. Quantitation of the viral DNA loads was accurate, as demonstrated by comparing the CPV DNA titres to those calculated by means of the TaqMan assay recognising all CPV types. This assay will ensure resolution of most diagnostic problems in dogs showing CPV disease shortly after CPV vaccination, although it does not discriminate between field strains and type 2b-based vaccines, recently licensed to market in some countries.

Metrological tests of a 200 L calibration source for HPGE detector systems for assay of radioactive waste drums

International Nuclear Information System (INIS)

Boshkova, T.; Mitev, K.

2016-01-01

In this work we present test procedures, approval criteria and results from two metrological inspections of a certified large volume "1"5"2Eu source (drum about 200 L) intended for calibration of HPGe gamma assay systems used for activity measurement of radioactive waste drums. The aim of the inspections was to prove the stability of the calibration source during its working life. The large volume source was designed and produced in 2007. It consists of 448 identical sealed radioactive sources (modules) apportioned in 32 transparent plastic tubes which were placed in a wooden matrix which filled the drum. During the inspections the modules were subjected to tests for verification of their certified characteristics. The results show a perfect compliance with the NIST basic guidelines for the properties of a radioactive certified reference material (CRM) and demonstrate the stability of the large volume CRM-drum after 7 years of operation. - Highlights: • Large (200 L) volume drum source designed, produced and certified as CRM in 2007. • Source contains 448 identical sealed radioactive "1"5"2Eu sources (modules). • Two metrological inspections in 2011 and 2014. • No statistically significant changes of the certified characteristics over time. • Stable calibration source for HPGe-gamma radioactive waste assay systems.

Kinetic assays for determining in vitro APS reductase activity in plants without the use of radioactive substances.

Science.gov (United States)

Brychkova, Galina; Yarmolinsky, Dmitry; Sagi, Moshe

2012-09-01

Adenosine 5'-phosphosulfate (APS) reductase (APR; EC 1.8.4.9) catalyzes the two-electron reduction of APS to sulfite and AMP, a key step in the sulfate assimilation pathway in higher plants. In spite of the importance of this enzyme, methods currently available for detection of APR activity rely on radioactive labeling and can only be performed in a very few specially equipped laboratories. Here we present two novel kinetic assays for detecting in vitro APR activity that do not require radioactive labeling. In the first assay, APS is used as substrate and reduced glutathione (GSH) as electron donor, while in the second assay APS is replaced by an APS-regenerating system in which ATP sulfurylase catalyzes APS in the reaction medium, which employs sulfate and ATP as substrates. Both kinetic assays rely on fuchsin colorimetric detection of sulfite, the final product of APR activity. Incubation of the desalted protein extract, prior to assay initiation, with tungstate that inhibits the oxidation of sulfite by sulfite oxidase activity, resulted in enhancement of the actual APR activity. The reliability of the two methods was confirmed by assaying leaf extract from Arabidopsis wild-type and APR mutants with impaired or overexpressed APR2 protein, the former lacking APR activity and the latter exhibiting much higher activity than the wild type. The assays were further tested on tomato leaves, which revealed a higher APR activity than Arabidopsis. The proposed APR assays are highly specific, technically simple and readily performed in any laboratory.

Technology Transfer of Isotopes-Based Assay: Strategies and Mechanisms

International Nuclear Information System (INIS)

Tabbada, R.S.D.C.; Rañada, M.L.O.; Mendoza, A.D.L.; Panganiban, R.; Castañeda, S.S.; Sombrito, E.Z.; Arcamo, S.V.R.

2015-01-01

Receptor Binding Assay for Paralytic Shellfish Poisoning (PSP RBA) is an isotope-based assay for detection and quantification of PSP toxins in seafood. It was established in the Philippines through a national program based on the recommendations of the Expert Mission sent by the International Atomic Energy Agency (IAEA). Through the said program, the Philippines Nuclear Research Institute (PNRI) was able to put up an RBA facility and develop expertise. Advantages of the technique against Mouse Bioassay (MBA) and high-performance Liquid Chromatography (HPLC) methods were are established. RBA is being utilized by some developed countries as screening method for Harmful Algal Bloom (HAB) Monitoring. However, it was not immediately adopted by the national HAB regulatory body for the following reasons: (1) acceptance of RBA as an official national method of analysis for PSP, (2) logistics and financial concerns in building up and maintaining a RBA facility, (3) considerations on the use of radioactive materials. To address these issues, the Philippines Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD) approved a Grants-In-Aid Project to initiate and to facilitate the transfer of the RBA technology to the monitoring and regulatory body. The project has two major objectives: capacity building and technology transfer. The capacity building focuses on human resources development of HAB monitoring personnel, specifically training on RBA and on the use of radioactive materials. On the other hand, the technology transfer deals with assistance that PNRI may render in establishing the new RBA facility and over-all know-how of the project. In this is poster, the mechanisms and strategies being undertaken by PNRI, in collaboration with the regulatory and monitoring body, to address the limitation of transferring a technology that utilizes radioactive materials including the technical difficulties are presented and discussed. (author)

Establishment of a non-radioactive cleavage assay to assess the DNA repair capacity towards oxidatively damaged DNA in subcellular and cellular systems and the impact of copper

International Nuclear Information System (INIS)

Hamann, Ingrit; Schwerdtle, Tanja; Hartwig, Andrea

2009-01-01

Oxidative stress is involved in many diseases, and the search for appropriate biomarkers is one major focus in molecular epidemiology. 8-Oxoguanine (8-oxoG), a potentially mutagenic DNA lesion, is considered to be a sensitive biomarker for oxidative stress. Another approach consists in assessing the repair capacity towards 8-oxoG, mediated predominantly by the human 8-oxoguanine DNA glycosylase 1 (hOGG1). With respect to the latter, during the last few years so-called cleavage assays have been described, investigating the incision of 32 P-labelled and 8-oxoG damaged oligonucleotides by cell extracts. Within the present study, a sensitive non-radioactive test system based on a Cy5-labelled oligonucleotide has been established. Sources of incision activity are isolated proteins or extracts prepared from cultured cells and peripheral blood mononuclear cells (PBMC). After comparing different oligonucleotide structures, a hairpin-like structure was selected which was not degraded by cell extracts. Applying this test system the impact of copper on the activity of isolated hOGG1 and on hOGG activity in A549 cells was examined, showing a distinct inhibition of the isolated protein at low copper concentration as compared to a modest inhibition of hOGG activity in cells at beginning cytotoxic concentrations. For investigating PBMC, all reaction conditions, including the amounts of oligonucleotide and cell extract as well as the reaction time have been optimized. The incision activities of PBMC protein extracts obtained from different donors have been investigated, and inter-individual differences have been observed. In summary, the established method is as sensitive and even faster than the radioactive technique, and additionally, offers the advantage of reduced costs and low health risk.

Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay

Directory of Open Access Journals (Sweden)

L Urdaneta

1998-09-01

Full Text Available Since 1984, DNA tests based on the highly repeated subtelomeric sequences of Plasmodium falciparum (rep 20 have been frequently used in malaria diagnosis. Rep 20 is very specific for this parasite, and is made of 21 bp units, organized in repeated blocks with direct and inverted orientation. Based in this particular organization, we selected a unique consensus oligonucleotide (pf-21 to drive a PCR reaction coupled to hybridization to non-radioactive labeled probes. The pf-21 unique oligo PCR (pf-21-I assay produced DNA amplification fingerprints when was applied on purified P. falciparum DNA samples (Brazil and Colombia, as well as in patient's blood samples from a large area of Venezuela. The performance of the Pf-21-I assay was compared against Giemsa stained thick blood smears from samples collected at a malaria endemic area of the Bolívar State, Venezuela, at the field station of Malariología in Tumeremo. Coupled to non-radioactive hybridization the pf-21-I performed better than the traditional microscopic method with a r=1.7:1. In the case of mixed infections the r value of P. falciparum detection increased to 2.5:1. The increased diagnostic sensitivity of the test produced with this homologous oligonucleotide could provide an alternative to the epidemiological diagnosis of P. falciparum being currently used in Venezuela endemic areas, where low parasitemia levels and asymptomatic malaria are frequent. In addition, the DNA fingerprint could be tested in molecular population studies

Comparison of cell-based and non-cell-based assay platforms for the detection of clinically relevant anti-drug neutralizing antibodies for immunogenicity assessment of therapeutic proteins.

Science.gov (United States)

Hu, Jenny; Wala, Iwona; Han, Hong; Nagatani, Janice; Barger, Troy; Civoli, Francesca; Kaliyaperumal, Arunan; Zhuang, Yao; Gupta, Shalini

2015-04-01

Anti-drug neutralizing antibodies (NAbs) formed due to unwanted immunogenicity of a therapeutic protein point towards a mature immune response. NAb detection is important in interpreting the therapeutic's efficacy and safety in vivo. In vitro cell-based NAb assays provide a physiological system for NAb detection, however are complex assays. Non-cell-based competitive ligand binding (CLB) approaches are also employed for NAb detection. Instead of cells, CLB assays use soluble receptor and conjugated reagents and are easier to perform, however have reduced physiological relevance. The aim of this study was to compare the performance of CLB assays to established cell-based assays to determine the former's ability to detect clinically relevant NAbs towards therapeutics that (i) acted as an agonist or (ii) acted as antagonists by binding to a target receptor. We performed a head-to-head comparison of the performance of cell-based and CLB NAb assays for erythropoietin (EPO) and two anti-receptor monoclonal antibodies (AMG-X and AMG 317). Clinically relevant NAb-positive samples identified previously by a cell-based assay were assessed in the corresponding CLB format(s). A panel of 12 engineered fully human anti-EPO monoclonal antibodies (MAbs) was tested in both EPO NAb assay formats. Our results showed that the CLB format was (i) capable of detecting human anti-EPO MAbs of differing neutralizing capabilities and affinities and (ii) provided similar results as the cell-based assay for detecting NAbs in patient samples. The cell-based and CLB assays also behaved comparably in detecting NAbs in clinical samples for AMG-X. In the case of anti-AMG 317 NAbs, the CLB format failed to detect NAbs in more than 50% of the tested samples. We conclude that assay sensitivity, drug tolerance and the selected assay matrix played an important role in the inability of AMG 317 CLB assays to detect clinically relevant NAbs. Copyright © 2015 Elsevier B.V. All rights reserved.

Chromatin structure influence of DNA damage measurements by four assays: pulsed- and constant-field gel electrophoresis, DNA precipitation and non-denaturing filter elution

International Nuclear Information System (INIS)

Wlodek, D.; Olive, P.L.

1996-01-01

The of elution of DNA during non-denaturing filter elution (NFE) often correlates with cell sensitivity to radiation. The elution rate is influenced by two cellular factors: chromatin structure and the number of DNA-strand breaks (DSBs) produced in an intact cell by ionizing radiation. To determine which of the above factors is relevant to cell radiosensitivity, four assays were used to measure induction of DNA damage in three cell lines varying in radiosensitivity (V79, CHO, and L5178Y-R). Each of the assays, neutral filter elution (NFE), DNA precipitation, constant (CFGE) and pulsed field gel electrophoresis (PFGE) have different physical basis for DNA damage measurement and might be differently affected by chromatin structure. Three of the methods used to measure DNA double-strand breaks gave different results: NFE was dependent on cell type and location of DNA relative to the replication fork, gel electrophoresis was independent of cell type but was affected by proximity to the replication fork, and the precipitation assay was independent of both cell type and replication status. Pulsed field gel electrophoresis produced the same results and constant field gel electrophoresis for 3 cell lines examined. Only NFE showed differences in sensitivity which correlated with cell survival following irradiation. The results suggest that three is the same initial amount of DSBs in cells from all three lines and that the sensitivity to radiation is determined by some additional factors, probably chromatin structure. (author). 18 refs, 5 figs

Non radioactive precursor import into chloroplasts

International Nuclear Information System (INIS)

Lombardo, V.A.; Ottado, J.

2003-01-01

Full text: Eukaryotic cells have a subcellular organization based on organelles. Protein transport to these organelles is quantitatively important because the majority of cellular proteins are codified in nuclear genes and then delivered to their final destination. Most of the chloroplast proteins are translated on cytoplasmic ribosomes as larger precursors with an amino terminal transit peptide that is necessary and sufficient to direct the precursor to the chloroplast. Once inside the organelle the transit peptide is cleaved and the mature protein adopts its folded form. In this work we developed a system for the expression and purification of the pea ferredoxin-NADP + reductase precursor (preFNR) for its import into chloroplasts in non radioactive conditions. We constructed a preFNR fused in its carboxy terminus to a 6 histidines peptide (preFNR-6xHis) that allows its identification using a commercial specific antibody. The construction was expressed, purified, processed and precipitated, rendering a soluble and active preFNR-6xHis that was used in binding and import into chloroplasts experiments. The reisolated chloroplasts were analyzed by SDS-PAGE, electro-blotting and revealed by immuno-detection using either colorimetric or chemiluminescent reactive. We performed also import experiments labeling preFNR and preFNR-6xHis with radioactive methionine as controls. We conclude that preFNR-6xHis is bound and imported into chloroplasts as the wild type preFNR and that both colorimetric or chemiluminescent detection methods are useful to avoid the manipulation of radioactive material. (author)

National policy for control of radioactive sources and radioactive waste from non-power applications in Lithuania

International Nuclear Information System (INIS)

Klevinskas, G.; Mastauskas, A.

2001-01-01

According to the Law on Radiation Protection of the Republic of Lithuania (passed in 1999), the Radiation Protection Centre of the Ministry of Health is the regulatory authority responsible for the radiation protection of public and of workers using sources of ionizing radiation in Lithuania. One of its responsibilities is the control of radioactive sources from the beginning of their 'life cycle', when they are imported in, used, transported and placed as spent into the radioactive waste storage facilities. For the effective control of sources there is national authorization system (notification- registration-licensing) based on the international requirements and recommendations introduced, which also includes keeping and maintaining the Register of Sources, controlling and investigating events while illegally carrying on or in possession of radioactive material, decision making and performing the state radiation protection supervision and control of users of radioactive sources, controlling, within the limits of competence, the radioactive waste management activities in nuclear and non-nuclear power applications. According to the requirements set out in the Law on Radiation Protection and the Government Resolution 'On Establishment of the State Register of the Sources of Ionizing Radiation and Exposure of Workers' (1999) and supplementary legal acts, all licence-holders conducting their activities with sources of ionizing radiation have to present all necessary data to the State Register after annual inventory of sources, after installation of new sources, after decommissioning of sources, after disposal of spent sources, after finishing the activities with the generators of ionizing radiation. The information to the Radiation Protection Centre has to be presented every week from the Customs Department of the Ministry of Finance about all sources of ionizing radiation imported to or exported from Lithuania and the information about the companies performed these

A quantification method for peroxyacetyl nitrate (PAN) using gas chromatography (GC) with a non-radioactive pulsed discharge detector (PDD)

Science.gov (United States)

Zhang, Lei; Jaffe, Daniel A.; Gao, Xin; McClure, Crystal D.

2018-04-01

In this study, we developed a method for continuous PAN measurements by gas chromatography (GC) with a non-radioactive pulsed discharge detector (PDD). Operational parameters were optimized based on the ratio of peak height over baseline noise (P/N ratio). The GC/PDD system was compared with a traditional radioactive electron-capture detector (ECD). In the lab, the method detection limit (MDL) of the new GC/PDD method (9 pptv) was lower than the radioactive GC/ECD method (15 pptv), demonstrating its excellent potential. The MDL of GC/PDD in the field campaign at the Mt. Bachelor Observatory (MBO) was 23 pptv, higher than in the lab. This was caused in part by the decreased slope of the calibration curve resulting from the low air pressure level at MBO. However, the MDL level of GC/PDD at MBO is still low enough for accurate PAN measurements, although special attention should be paid to its application at high-elevation sites. Observations of PAN were conducted at MBO in the summer of 2016 with the GC/PDD system, and provided more evidence of the performance of the system. PAN was found to be highly correlated with CO. The promising performance of GC/PDD which does not require a radioactive source makes it a useful approach for accurate PAN measurements in the field.

Destructive and non-destructive tests for radioactive waste packages Task 3 Characterization of radioactive waste forms. A series of final reports (1985-89) No 43

International Nuclear Information System (INIS)

Odoj, R.

1991-01-01

On the basis of preliminary waste acceptance requirements quality control of radioactive waste has to be performed prior to interim storage or final disposal. The quality control can either be achieved by random tests on conditioned radioactive waste packages or by process qualification of the conditioning processes. One of the most important criteria is the activity of the radioactive waste product or packages. To get some first information on the waste package γ-spectrometric measurement is performed as non-destructive test. Besides the γ-emitting nuclides the α and β-emitting nuclides can be estimated by calculation if the waste was generated in nuclear power plants and the nuclide relations are known. If the non-destructive determination of nuclides is not sufficient or the non-radioactive content of the waste packages has to be identified sampling from the waste packages has to be performed. This can best be done by core drilling. To avoid the need of water for cooling the drill head, air cooled core drilling is investigated. As mixed wastes is not allowed for final disposal the determination of possible organic toxic materials like PCB, dioxin and furane-compounds in cemented wastes is conducted by GC-MS-investigations. For getting more knowledge in the field of process qualification concerning super compaction, instrumentation of the super compaction process is investigated and tested

Radioactive waste package assay facility. Final report - V. A

International Nuclear Information System (INIS)

Molesworth, T.V.; Strachan, N.R.; Findlay, D.J.S.; Wise, M.O.; Forrest, K.R.; Rogers, J.D.

1993-01-01

This report provides a summary of research work carried out in support of the development of an integrated assay system for the quality checking of Intermediate Level Waste encapsulated in cement. Four non-destructive techniques were originally identified as being viable methods for obtaining radiometric inventory data from a cemented 500 litre ILW package. The major part of the programme was devoted to the development of two interrogation techniques; active neutron for measuring the total fissile content and active gamma for measuring the total actinide content. An electron linear accelerator was used to supply the interrogating beam for these two methods. In addition the linear accelerator beam could be used for high energy radiography. The results of this work are described and the performances and limitations of the non-destructive methods are summarised. The main engineering and operational features which influence the design of an integrated assay facility are outlined and a conceptual layout for a facility to inspect 750 ILW drums a year is described. Details of the detection methods, data processing and potential application of the assay facility are given in three associated HMIP reports. (Author)

Criteria and Processes for the Certification of Non-Radioactive Hazardous and Non-Hazardous Wastes

International Nuclear Information System (INIS)

Dominick, J.

2008-01-01

This document details Lawrence Livermore National Laboratory's (LLNL) criteria and processes for determining if potentially volumetrically contaminated or potentially surface contaminated wastes are to be managed as material containing residual radioactivity or as non-radioactive. This document updates and replaces UCRL-AR-109662, Criteria and Procedures for the Certification of Nonradioactive Hazardous Waste (Reference 1), also known as 'The Moratorium', and follows the guidance found in the U.S. Department of Energy (DOE) document, Performance Objective for Certification of Non-Radioactive Hazardous Waste (Reference 2). The 1992 Moratorium document (UCRL-AR-109662) is three volumes and 703 pages. The first volume provides an overview of the certification process and lists the key radioanalytical methods and their associated Limits of Sensitivities. Volumes Two and Three contain supporting documents and include over 30 operating procedures, QA plans, training documents and organizational charts that describe the hazardous and radioactive waste management system in place in 1992. This current document is intended to update the previous Moratorium documents and to serve as the top-tier LLNL institutional Moratorium document. The 1992 Moratorium document was restricted to certification of Resource Conservation and Recovery Act (RCRA), State and Toxic Substances Control Act (TSCA) hazardous waste from Radioactive Material Management Areas (RMMA). This still remains the primary focus of the Moratorium; however, this document increases the scope to allow use of this methodology to certify other LLNL wastes and materials destined for off-site disposal, transfer, and re-use including non-hazardous wastes and wastes generated outside of RMMAs with the potential for DOE added radioactivity. The LLNL organization that authorizes off-site transfer/disposal of a material or waste stream is responsible for implementing the requirements of this document. The LLNL Radioactive and

Expert system for transuranic waste assay

Energy Technology Data Exchange (ETDEWEB)

Zoolalian, M.L.; Gibbs, A.; Kuhns, J.D.

1989-01-01

Transuranic wastes are generated at the Savannah River Site (SRS) as a result of routine production of nuclear materials. These wastes contain Pu-238 and Pu-239 and are placed into lined 55-gallon waste drums. The drums are placed on monitored storage pads pending shipment to the Waste Isolation Pilot Plant in New Mexico. A passive-active neutron (PAN) assay system is used to determine the mass of the radioactive material within the waste drums. Assay results are used to classify the wastes as either low-level or transuranic (TRU). During assays, the PAN assay system communicates with an IBM-AT computer. A Fortran computer program, called NEUT, controls and performs all data analyses. Unassisted, the NEUT program cannot adequately interpret assay results. To eliminate this limitation, an expert system shell was used to write a new algorithm, called the Transuranic Expert System (TRUX), to drive the NEUT program and add decision making capabilities for analysis of the assay results. The TRUX knowledge base was formulated by consulting with human experts in the field of neutron assay, by direct experimentation on the PAN assay system, and by observing operations on a daily basis. TRUX, with its improved ability to interpret assay results, has eliminated the need for close supervision by a human expert, allowing skilled technicians to operate the PAN assay system. 4 refs., 1 fig., 4 tabs.

Expert system for transuranic waste assay

International Nuclear Information System (INIS)

Zoolalian, M.L.; Gibbs, A.; Kuhns, J.D.

1989-01-01

Transuranic wastes are generated at the Savannah River Site (SRS) as a result of routine production of nuclear materials. These wastes contain Pu-238 and Pu-239 and are placed into lined 55-gallon waste drums. The drums are placed on monitored storage pads pending shipment to the Waste Isolation Pilot Plant in New Mexico. A passive-active neutron (PAN) assay system is used to determine the mass of the radioactive material within the waste drums. Assay results are used to classify the wastes as either low-level or transuranic (TRU). During assays, the PAN assay system communicates with an IBM-AT computer. A Fortran computer program, called NEUT, controls and performs all data analyses. Unassisted, the NEUT program cannot adequately interpret assay results. To eliminate this limitation, an expert system shell was used to write a new algorithm, called the Transuranic Expert System (TRUX), to drive the NEUT program and add decision making capabilities for analysis of the assay results. The TRUX knowledge base was formulated by consulting with human experts in the field of neutron assay, by direct experimentation on the PAN assay system, and by observing operations on a daily basis. TRUX, with its improved ability to interpret assay results, has eliminated the need for close supervision by a human expert, allowing skilled technicians to operate the PAN assay system. 4 refs., 1 fig., 4 tabs

Proceedings of the NEA Workshop on the Management of Non-Nuclear Radioactive Waste

International Nuclear Information System (INIS)

Zafiropoulos, Demetre; Dilday, Daniel; Siemann, Michael; Ciambrella, Massimo; Lazo, Edward; Sartori, Enrico; ); Dionisi, Mario; Long, Juliet; Nicholson, David; Chambers, Douglas; Garcia Alves, Joao Henrique; McMahon, Ciara; Bruno, Gerard; Fan, Zhiwen; ); Ripani, Marco; Nielsen, Mette; Solente, Nicolas; Templeton, John; Paratore, Angelo; Feinhals, Joerg; Pandolfi, Dana; Sarchiapone, Lucia; Picentino, Bruno; Simms, Helen; Beer, Hans-Frieder; Deryabin, Sergey; Ulrici, Luisa; Bergamaschi, Carlo; Nottestad, Stacy; Anagnostakis, Marios

2017-05-01

All NEA member countries, whether or not they have nuclear power plants, are faced with appropriately managing non-nuclear radioactive waste produced through industrial, research and medical activities. Sources of such waste can include national laboratory and university research activities, used and lost industrial gauges and radiography sources, hospital nuclear medicine activities and in some circumstances, naturally occurring radioactive material (NORM) activities. Although many of these wastes are not long-lived, the shear variety of sources makes it difficult to generically assess their physical (e.g. volume, chemical form, mixed waste) or radiological (e.g. activity, half-life, concentration) characteristics. Additionally, the source-specific nature of these wastes poses questions and challenges to their regulatory and practical management at a national level. This had generated interest from both the radiological protection and radioactive waste management communities, and prompted the Committee on Radiological Protection and Public Health (CRPPH) to organise, in collaboration with the Radioactive Waste Management Committee (RWMC), a workshop tackling some of the key issues of this challenging topic. The key objectives of the NEA Workshop on the Management of Non-Nuclear Radioactive Waste were to address the particularities of managing non-nuclear waste in all its sources and forms and to share and exchange national experiences. Presentations and discussions addressed both technical aspects and national frameworks. Technical aspects included: - the range of non-nuclear waste sources, activities, volumes and other relevant characteristics; - waste storage and repository capacities and life cycles; - safety considerations for mixed wastes management; - human resources and knowledge management; - legal, regulatory and financial assurance, and liability issues. Taking into account the entire non-nuclear waste life-cycle, the workshop covered planning and

EL4 cell-based colorimetric toxin neutralization activity assays for determination of neutralizing anti-ricin antibodies.

Science.gov (United States)

Lindsey, Changhong Y; Brown, J Edward; Torabazar, Nahid R; Smith, Leonard A

2013-01-01

A recombinant ricin toxin A-chain 1-33/44-198 vaccine (RVEc), developed at the United States Army Medical Research Institute of Infectious Diseases as a vaccine candidate, is under investigation in a phase 1 clinical study. To effectively evaluate the immunogenicity of this ricin vaccine and to eliminate the use of radioactive material, an EL4 cell-based colorimetric toxin neutralization activity (TNA) assay using a CellTiter 96 AQueous One Solution Cell Proliferation Assay Reagent has been developed, optimized, and applied in the vaccine efficacy studies. The TNA assay measures the protective neutralizing anti-ricin antibodies in animal sera by determining the cell viability after ricin exposure in the assay system and comparing it to a purified mouse polyclonal antiricin IgG standard curve. The standard curve of the anti-ricin TNA assay closely fits a four-parameter logistic regression model. The unknown test sample concentration was expressed as microg/mL, but not the 50% effective concentration (EC50), which was determined by most TNA assays. The neutralizing endpoint titers, not the 50% effective dilution (ED50), of human specimens were measured with the TNA assay in support of the clinical study of the RVEc vaccine. The optimal amount of ricin toxin, EL4 cells, and concentration of standards used in the assay system was established to minimize false-negative and false-positive results of serum specimens from the nonclinical and clinical studies of RVEc. The testing conditions were adjusted to optimize assay performance. The colorimetric TNA assay replaced a radioactive TNA assay previously used in the ricin vaccine studies.

Development of an expert system for radioactive material transportation

International Nuclear Information System (INIS)

Tamanoi, K.; Ishitobi, M.; Shinohara, Y.

1990-01-01

An expert system to deal with radioactive material transportation was developed. This expert system is based on 'Regulations for the Safe Transport of Radioactive Material' by IAEA issued 1985. IAEA published the regulations under such environments that safety transportation has become increasingly being focused as uses of radioactive materials are more pervasive, not only in nuclear field but also in non-nuclear purposes. Attentions are payed for operators and environment to establish safety in handling radioactive materials. In the 1985 regulations, detailed categorization of radioactive materials and, correspondingly, new classification of packages are introduced. This categorization is more complicated than old regulations, leading us to develop an expert system to evaluate easily the packages categorization. (author)

Microbead agglutination based assays

KAUST Repository

Kodzius, Rimantas

2013-01-21

We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

Assay for intrinsic factor based on blocking of the R binder of gastric juice by cobinamide

International Nuclear Information System (INIS)

Begley, J.A.; Trachtenberg, A.

1979-01-01

An in vitro assay for measurement of gastric juice intrinsic factor (IF) was developed based on the ability of the cobinamide (Cbi) [(CN, OH) Cbi] to bind to the gastric juice R-type binders of cobalamin (Cbl) and not to the IF binder. Subsequently added radioactive Cbl, CN-[ 57 Co] Cbl, binds only to the IF binders and allows for direct measurement of this Cbl binding protein. This Cbi blocking assay was found to function as well as the more conventional methods of IF measurement, G-100 column chromatography, and IF blocking antibody assay. The present assay has the advantage of eliminating the need for elaborate forms of protein separation and does not rely on a source of antibody

Improvement of non-destructive fissile mass assays in α low-level waste drums: A matrix correction method based on neutron capture gamma-rays and a neutron generator

Science.gov (United States)

Jallu, F.; Loche, F.

2008-08-01

Within the framework of radioactive waste control, non-destructive assay (NDA) methods may be employed. The active neutron interrogation (ANI) method is now well-known and effective in quantifying low α-activity fissile masses (mainly 235U, 239Pu, 241Pu) with low densities, i.e. less than about 0.4, in radioactive waste drums of volumes up to 200 l. The PROMpt Epithermal and THErmal interrogation Experiment (PROMETHEE [F. Jallu, A. Mariani, C. Passard, A.-C. Raoux, H. Toubon, Alpha low level waste control: improvement of the PROMETHEE 6 assay system performances. Nucl. Technol. 153 (January) (2006); C. Passard, A. Mariani, F. Jallu, J. Romeyer-Dherber, H. Recroix, M. Rodriguez, J. Loridon, C. Denis, PROMETHEE: an alpha low level waste assay system using passive and active neutron measurement methods. Nucl. Technol. 140 (December) (2002) 303-314]) based on ANI has been under development since 1996 to reach the incinerating α low level waste (LLW) criterion of about 50 Bq[α] per gram of crude waste (≈50 μg Pu) in 118 l drums on the date the drums are conditioned. Difficulties arise when dealing with matrices containing neutron energy moderators such as H and neutron absorbents such as Cl. These components may have a great influence on the fissile mass deduced from the neutron signal measured by ANI. For example, the calibration coefficient measured in a 118 l drum containing a cellulose matrix (density d = 0.144 g cm -3) may be 50 times higher than that obtained in a poly-vinyl-chloride matrix ( d = 0.253 g cm -3). Without any information on the matrix, the fissile mass is often overestimated due to safety procedures and by considering the most disadvantageous calibration coefficient corresponding to the most absorbing and moderating calibration matrix. The work discussed in this paper was performed at the CEA Nuclear Measurement Laboratory in France. It concerns the development of a matrix effect correction method, which consists in identifying and quantifying

Improvement of non-destructive fissile mass assays in α low-level waste drums: A matrix correction method based on neutron capture gamma-rays and a neutron generator

International Nuclear Information System (INIS)

Jallu, F.; Loche, F.

2008-01-01

Within the framework of radioactive waste control, non-destructive assay (NDA) methods may be employed. The active neutron interrogation (ANI) method is now well-known and effective in quantifying low α-activity fissile masses (mainly 235 U, 239 Pu, 241 Pu) with low densities, i.e. less than about 0.4, in radioactive waste drums of volumes up to 200 l. The PROMpt Epithermal and THErmal interrogation Experiment (PROMETHEE [F. Jallu, A. Mariani, C. Passard, A.-C. Raoux, H. Toubon, Alpha low level waste control: improvement of the PROMETHEE 6 assay system performances. Nucl. Technol. 153 (January) (2006); C. Passard, A. Mariani, F. Jallu, J. Romeyer-Dherber, H. Recroix, M. Rodriguez, J. Loridon, C. Denis, PROMETHEE: an alpha low level waste assay system using passive and active neutron measurement methods. Nucl. Technol. 140 (December) (2002) 303-314]) based on ANI has been under development since 1996 to reach the incinerating α low level waste (LLW) criterion of about 50 Bq[α] per gram of crude waste (∼50 μg Pu) in 118 l drums on the date the drums are conditioned. Difficulties arise when dealing with matrices containing neutron energy moderators such as H and neutron absorbents such as Cl. These components may have a great influence on the fissile mass deduced from the neutron signal measured by ANI. For example, the calibration coefficient measured in a 118 l drum containing a cellulose matrix (density d = 0.144 g cm -3 ) may be 50 times higher than that obtained in a poly-vinyl-chloride matrix (d = 0.253 g cm -3 ). Without any information on the matrix, the fissile mass is often overestimated due to safety procedures and by considering the most disadvantageous calibration coefficient corresponding to the most absorbing and moderating calibration matrix. The work discussed in this paper was performed at the CEA Nuclear Measurement Laboratory in France. It concerns the development of a matrix effect correction method, which consists in identifying and

Improvement of non-destructive fissile mass assays in {alpha} low-level waste drums: A matrix correction method based on neutron capture gamma-rays and a neutron generator

Energy Technology Data Exchange (ETDEWEB)

Jallu, F. [Commissariat a l' Energie Atomique, CEA, DEN, Nuclear Measurement Laboratory, Bat. 224, 13108 Saint Paul lez Durance (France)], E-mail: fanny.jallu@cea.fr; Loche, F. [Commissariat a l' Energie Atomique, CEA, DEN, Nuclear Measurement Laboratory, Bat. 224, 13108 Saint Paul lez Durance (France)

2008-08-15

Within the framework of radioactive waste control, non-destructive assay (NDA) methods may be employed. The active neutron interrogation (ANI) method is now well-known and effective in quantifying low {alpha}-activity fissile masses (mainly {sup 235}U, {sup 239}Pu, {sup 241}Pu) with low densities, i.e. less than about 0.4, in radioactive waste drums of volumes up to 200 l. The PROMpt Epithermal and THErmal interrogation Experiment (PROMETHEE [F. Jallu, A. Mariani, C. Passard, A.-C. Raoux, H. Toubon, Alpha low level waste control: improvement of the PROMETHEE 6 assay system performances. Nucl. Technol. 153 (January) (2006); C. Passard, A. Mariani, F. Jallu, J. Romeyer-Dherber, H. Recroix, M. Rodriguez, J. Loridon, C. Denis, PROMETHEE: an alpha low level waste assay system using passive and active neutron measurement methods. Nucl. Technol. 140 (December) (2002) 303-314]) based on ANI has been under development since 1996 to reach the incinerating {alpha} low level waste (LLW) criterion of about 50 Bq[{alpha}] per gram of crude waste ({approx}50 {mu}g Pu) in 118 l drums on the date the drums are conditioned. Difficulties arise when dealing with matrices containing neutron energy moderators such as H and neutron absorbents such as Cl. These components may have a great influence on the fissile mass deduced from the neutron signal measured by ANI. For example, the calibration coefficient measured in a 118 l drum containing a cellulose matrix (density d = 0.144 g cm{sup -3}) may be 50 times higher than that obtained in a poly-vinyl-chloride matrix (d = 0.253 g cm{sup -3}). Without any information on the matrix, the fissile mass is often overestimated due to safety procedures and by considering the most disadvantageous calibration coefficient corresponding to the most absorbing and moderating calibration matrix. The work discussed in this paper was performed at the CEA Nuclear Measurement Laboratory in France. It concerns the development of a matrix effect correction

Radioactive waste from non-licensed activities - identification of waste, compilation of principles and guidance, and proposed system for final management

International Nuclear Information System (INIS)

Jones, C.; Pers, K.

2001-07-01

Presently national guidelines for the handling of radioactive waste from non-licensed activities are lacking in Sweden. Results and information presented in this report are intended to form a part of the basis for decisions on further work within the Swedish Radiation Protection Institute on regulations or other guidelines on final management and final disposal of this type of waste. An inventory of radioactive waste from non-licensed activities is presented in the report. In addition, existing rules and principles used in Sweden - and internationally - on the handling of radioactive and toxic waste and non-radioactive material are summarized. Based on these rules and principles a system is suggested for the final management of radioactive material from non-licensed activities. A model is shown for the estimation of dose as a consequence of leaching of radio-nuclides from different deposits. The model is applied on different types of waste, e.g. peat ashes, light concrete and low-level waste from a nuclear installation

Training of personnel in the field of radioactive materials transport

International Nuclear Information System (INIS)

Fasten, Ch.

1997-01-01

Training of personnel in the whole nuclear fuel cycle and also in the other fields of the use of radioactivity is one of the essentials with respect to compliance assurance. The transport of radioactive material is the only activity that takes place outside a facility: on roads, on railways, on the sea or in the air. A high level of safety is therefore an absolute requirement for all transport operations. To ensure this high level the training of the personnel involved in these activities plays an important role. Many studies show that most of the incidents in radioactive materials transport are caused by man-made errors: even so there have been no events with serious radiological consequences anywhere worldwide. There are many requirements in the various national and international regulations for the safe transport of radioactive material with regard to training. An overview is given of the special regulations, e.g. for road transport drivers, for safety advisers in the whole field of the transport of dangerous goods, for specially educated personnel in sea and air transports. In addition, the newest developments in the European Community in this field are discussed. An evaluation of the present regulations and proposals for further rules are also given. (Author)

Size measurement of radioactive aerosol particles in intense radiation fields using wire screens and imaging plates

Energy Technology Data Exchange (ETDEWEB)

Oki, Yuichi; Tanaka, Toru; Takamiya, Koichi; Ishi, Yoshihiro; UesugI, Tomonori; Kuriyama, Yasutoshi; Sakamoto, Masaaki; Ohtsuki, Tsutomu [Kyoto University Research Reactor Institute, Osaka (Japan); Nitta, Shinnosuke [Graduate School of Engineering, Kyoto University, Kyoto (Japan); Osada, Naoyuki [Advanced Science Research Center, Okayama University, Okayama (Japan)

2016-09-15

Very fine radiation-induced aerosol particles are produced in intense radiation fields, such as high-intensity accelerator rooms and containment vessels such as those in the Fukushima Daiichi nuclear power plant (FDNPP). Size measurement of the aerosol particles is very important for understanding the behavior of radioactive aerosols released in the FDNPP accident and radiation safety in high-energy accelerators. A combined technique using wire screens and imaging plates was developed for size measurement of fine radioactive aerosol particles smaller than 100 nm in diameter. This technique was applied to the radiation field of a proton accelerator room, in which radioactive atoms produced in air during machine operation are incorporated into radiation-induced aerosol particles. The size of 11C-bearing aerosol particles was analyzed using the wire screen technique in distinction from other positron emitters in combination with a radioactive decay analysis. The size distribution for 11C-bearing aerosol particles was found to be ca. 70 μm in geometric mean diameter. The size was similar to that for 7Be-bearing particles obtained by a Ge detector measurement, and was slightly larger than the number-based size distribution measured with a scanning mobility particle sizer. The particle size measuring method using wire screens and imaging plates was successfully applied to the fine aerosol particles produced in an intense radiation field of a proton accelerator. This technique is applicable to size measurement of radioactive aerosol particles produced in the intense radiation fields of radiation facilities.

Adaptive assay of pulmonary radioactive aerosol with an external detector

International Nuclear Information System (INIS)

Talmor, A.

1989-12-01

The applicability of the adaptive assay method was examined and then used to reduce the error caused by non-uniform spatial distribution. A computer program simulates adaptive assay of pulmonary aerosol within a standard man lungs, and compares its results with the results of static measurement. In the extreme hypothetical situation in which the aerosol is concentrated entirely in the left lung, and the static measurement is performed under the right arm, the errors obtained by calibration the static measurement on assumption of uniform spatial distribution, is as large as a factor 5 of the true value. In the same situation the adaptive assay result errs by less than 20%. In another situation, in which the aerosol is distributed in both lungs, and its concentration is higher in the pleura and near the back, the error obtained by calibrating the static measurement on the assumption of uniform spatial distribution, is as large as 30%, while the adaptive assay result errs by less than 2%. (author)

Application of PINS and GNAT to the assay of 55-gal containers of radioactive waste

International Nuclear Information System (INIS)

Gehrke, R.J.; Aryaeinejad, R.; Watts, K.D.; Staples, D.R.; Akers, D.W.

1994-03-01

The Portable Isotropic Neutron Spectroscopy (PINS) and Gamma Neutron Assay Technique (GNAT) assay systems that were developed with funding from the office of Research and Development (NN20), were taken to the Stored Waste Examination Pilot Plant (SWEPP) facility at the Radioactive Waste Management Complex (RWMC) and applied to the assay of surrogate and Rocky Flats Plant waste contained in 55-gal drums. PINS, a portable prompt γ neutron activation analysis technique, was able to identify key elements in both the surrogate and real waste so that three-main waste categories: metal, combustible material, and cemented chlorinated sludge wastes could be identified. GNAT, a γ, neutron assay technique for the identification and quantification of fissioning isotopes, was able to identify 240 Pu in surrogate waste in which nine 1-g nuclear accident dosimeters were inserted. GNAT was also able to identify 24O Pu in real 55-gal waste drums containing 15- and 40-g of plutonium even in the presence of high activity concentrations of 241 Am

International conference on management of radioactive waste from non-power applications - Sharing the experience. Book of extended synopses

International Nuclear Information System (INIS)

2001-01-01

The primary objective of the conference is to provide an opportunity for experts in this field to meet and exchange information, and to discuss experience, specific practices and technical solutions used in the management of radioactive waste derived from different non-power applications. This includes waste from the operation of research reactors, and from the production and application of radioisotopes, labelled compounds and sealed radioactive sources in industry, medicine, agriculture, research and education. The discussion may also include management of specific waste types, such as waste from radiological accidents, waste from remediation activities connected with old, inadequate waste management facilities, etc. The conference may also address the issues of management of very low level radioactive waste (VLLRW) and of technologically enhanced naturally occurring radioactive materials (TENORMs) to identify the existing scale of the problems and to analyse current approaches of Member States to their solution. The conference is also intended to identify the most important and problematic components of the subject and to facilitate the sharing of experience in improving efficiency, safety and economy in the management of radioactive waste from non-power nuclear applications. This publication contains 89 extended synopses of the oral and poster presentations delivered at the conference. Each of them was indexed separately

Neutron Based Non-Destructive Assay (NDA) Measurement Systems for Safeguard

Energy Technology Data Exchange (ETDEWEB)

Swinhoe, Martyn Thomas [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-09-21

The objectives of this project are to introduce the assay methods for plutonium measurements using the HLNC; introduce the assay method for bulk uranium measurements using the AWCC; and introduce the assay method for fuel assembly measurements using the UNCL.

An indicator cell assay for blood-based diagnostics.

Directory of Open Access Journals (Sweden)

Samuel A Danziger

Full Text Available We have established proof of principle for the Indicator Cell Assay Platform™ (iCAP™, a broadly applicable tool for blood-based diagnostics that uses specifically-selected, standardized cells as biosensors, relying on their innate ability to integrate and respond to diverse signals present in patients' blood. To develop an assay, indicator cells are exposed in vitro to serum from case or control subjects and their global differential response patterns are used to train reliable, disease classifiers based on a small number of features. In a feasibility study, the iCAP detected pre-symptomatic disease in a murine model of amyotrophic lateral sclerosis (ALS with 94% accuracy (p-Value = 3.81E-6 and correctly identified samples from a murine Huntington's disease model as non-carriers of ALS. Beyond the mouse model, in a preliminary human disease study, the iCAP detected early stage Alzheimer's disease with 72% cross-validated accuracy (p-Value = 3.10E-3. For both assays, iCAP features were enriched for disease-related genes, supporting the assay's relevance for disease research.

Ratio methods for cost-effective field sampling of commercial radioactive low-level wastes

International Nuclear Information System (INIS)

Eberhardt, L.L.; Simmons, M.A.; Thomas, J.M.

1985-07-01

In many field studies to determine the quantities of radioactivity at commercial low-level radioactive waste sites, preliminary appraisals are made with field radiation detectors, or other relatively inaccurate devices. More accurate determinations are subsequently made with procedures requiring chemical separations or other expensive analyses. Costs of these laboratory determinations are often large, so that adequate sampling may not be achieved due to budget limitations. In this report, we propose double sampling as a way to combine the expensive and inexpensive aproaches to substantially reduce overall costs. The underlying theory was developed for human and agricultural surveys, and is partially based on assumptions that are not appropriate for commercial low-level waste sites. Consequently, extensive computer simulations were conducted to determine whether the results can be applied in circumstances of importance to the Nuclear Regulatory Commission. This report gives the simulation details, and concludes that the principal equations are appropriate for most studies at commercial low-level waste sites. A few points require further research, using actual commercial low-level radioactive waste site data. The final section of the report provides some guidance (via an example) for the field use of double sampling. Details of the simulation programs are available from the authors. Major findings are listed in the Executive Summary. 9 refs., 9 figs., 30 tabs

Radioactive waste from non-power applications in Sweden

International Nuclear Information System (INIS)

Haegg, Ann-Christin; Lindbom, Gunilla; Persson, Monica

2001-01-01

Full text: The system for handling of radioactive waste from the Nuclear Fuel Cycle in Sweden is well established and has been in use for many years. Radioactive waste from other sources is not always handled as rigorously. The Swedish Radiation Protection Institute, SSI has identified the issue and therefore initiated a study with the aim to achieve a sufficient system for handling and disposal of radioactive waste from all sources of radioactive waste. In this paper we discuss some of the sources of radioactive waste and the specific problems they represent. We give a brief description on how they are regulated and handled today and identify some interesting issues. Conventional industry, hospitals, research and education: In the conventional industry the use of different types of radioactive sources is common. The size and type of radioactive source depends on the application (from some megaBq up to thousands of terraBq). The radioactive waste from hospitals, research institutions and pharmaceutical or bio-technical industries consists mainly of very short-lived radionuclides. Also most sealed sources used in the medical field contains short-lived radionuclides. According to the Swedish Radiation Protection Act a licence is needed for the use of sealed sources exceeding 50 kiloBq. For hospitals and research institutes the SSI issues one license covering all radioactive sources below 500 megaBq up to a summary limit depending on the application. All sources with activity exceeding 500 megaBq require a separate license. SSI has issued about 2500 licences. For each licence an annual fee is paid to the SSI. When the radioactive source has fulfilled its purpose the licensee is obliged to inform the SSI that the source is no longer in use and show a certificate from the recognised waste facility. Not until this has been done the licensee is released from its responsibilities. SSI has issued regulations on Radioactive Waste Not Associated with Nuclear Energy. These

Survey on non-nuclear radioactive waste; Kartlaeggning av radioaktivt avfall fraan icke kaernteknisk verksamhet

Energy Technology Data Exchange (ETDEWEB)

NONE

2003-11-01

On request from the Swedish Radiation Protection Authority, the Swedish government has in May 2002 set up a non-standing committee for non-nuclear radioactive waste. The objective was to elaborate proposals for a national system for the management of all types of non-nuclear radioactive wastes with special consideration of inter alia the polluter pays principle and the responsibility of the producers. The committee will deliver its proposals to the government 1 December 2003. SSI has assisted the committee to the necessary extent to fulfill the investigation. This report is a summery of SSI's background material concerning non-nuclear radioactive waste in Sweden.

Local mat-forming cyanobacteria effectively facilitate decontamination of radioactive cesium in rice fields

International Nuclear Information System (INIS)

Yamamoto, Atsushi; Yoshida, Shigeru; Okumura, Hiroshi; Inagaki, Masayo; Yamanishi, Hirokuni; Ito, Tetsuo; Furukawa, Michio

2015-01-01

The most effective and widespread method to decontaminate radioactive cesium from the Fukushima Daiichi Nuclear Power Plant Disaster was peeling topsoil. But the method had problems, such as large amounts of discarded soil and large-scale work. In nature, cyanobacteria formed biomats on the ground surface and facilitated peeling topsoil when the biomats dried. The cyanobacteria-facilitating peeling decontamination method utilized these cyanobacterial properties. Cyanobacteria are located all over Japan and 'local' cyanobacteria could be used for decontamination without introducing new species. Utilizing cyanobacteria could decrease the amount of discarded soil to about 30% and downsize the execution-scale to individual locations. Cyanobacterial biomats were easily cultivated, especially in rice fields, by maintaining wet conditions and exposure to 100 - 83% solar radiation. Shading by a thin net was helpful in maintaining an environment suitable for cyanobacteria. Nowadays, to prevent uptake of radioactive cesium into rice, K + is usually added to fertilizer in rice fields. The K + fertilization in rice fields might also enhance cyanobacterial capture of radioactive cesium, because high concentrations of K + enhanced cyanobacterial uptake of Cs + . Cyanobacteria could also mitigate the risk of radioactive cesium moving away from a decontaminating rice field. Therefore, the cyanobacteria-facilitating peeling decontamination method was proposed as an easy and safe 'D.I.Y.' method for both farmers and the environment. Besides, plowing rice fields with water before peeling improved the efficiency of this method, because plowing increased the radioactive cesium concentration in the topsoil. (author)

Genotoxicity and ELF-magnetic fields: a review through the micronucleus assay

International Nuclear Information System (INIS)

Alcaraz, M.; Andreu-Galvez, M.; Sanchez-Villalobos, J. M.; Achel, D. G.; Olmos, E.; Martinez-Hernandez, C. M.

2012-01-01

Thirty for (34) published studies, conducted from 1994 to the present to evaluate the genotoxic effect of magnetic fields using ELF-EMF and diagnostic resonance on humans by the micronucleus assay have been reviewed. some characteristics of the assay methods, their significance to genotoxicity and basic interpretations of the results of these assays are discussed. of the studies analysed 70.5% implicated genotoxic effects induced by these magnetic fields: 52.9% were due to exposure to magnetic fields only and 17,6% by exposure to magnetic fields in combination with some treatment types, resulting in additive or synergistic effect. Evidence exist to support the notion that exposure of humans to magnetic fields stimulates genotoxic effects, although the actual mechanisms of action or even the true human health consequences resulting from these exposure still remain unclear. (Author) 80 refs.

Non-Enzymatic Detection of Bacterial Genomic DNA Using the Bio-Barcode Assay

Science.gov (United States)

Hill, Haley D.; Vega, Rafael A.; Mirkin, Chad A.

2011-01-01

The detection of bacterial genomic DNA through a non-enzymatic nanomaterials based amplification method, the bio-barcode assay, is reported. The assay utilizes oligonucleotide functionalized magnetic microparticles to capture the target of interest from the sample. A critical step in the new assay involves the use of blocking oligonucleotides during heat denaturation of the double stranded DNA. These blockers bind to specific regions of the target DNA upon cooling, and prevent the duplex DNA from re-hybridizing, which allows the particle probes to bind. Following target isolation using the magnetic particles, oligonucleotide functionalized gold nanoparticles act as target recognition agents. The oligonucleotides on the nanoparticle (barcodes) act as amplification surrogates. The barcodes are then detected using the Scanometric method. The limit of detection for this assay was determined to be 2.5 femtomolar, and this is the first demonstration of a barcode type assay for the detection of double stranded, genomic DNA. PMID:17927207

Mesoscale meteorological model based on radioactive explosion cloud simulation

International Nuclear Information System (INIS)

Zheng Yi; Zhang Yan; Ying Chuntong

2008-01-01

In order to simulate nuclear explosion and dirty bomb radioactive cloud movement and concentration distribution, mesoscale meteorological model RAMS was used. Particles-size, size-active distribution and gravitational fallout in the cloud were considered. The results show that the model can simulate the 'mushroom' clouds of explosion. Three-dimension fluid field and radioactive concentration field were received. (authors)

Radioactivity Measurements on Glazed Ceramic Surfaces

OpenAIRE

Hobbs, Thomas G.

2000-01-01

A variety of commonly available household and industrial ceramic items and some specialty glass materials were assayed by alpha pulse counting and ion chamber voltage measurements for radioactivity concentrations. Identification of radionuclides in some of the items was performed by gamma spectroscopy. The samples included tableware, construction tiles and decorative tiles, figurines, and other products with a clay based composition. The concentrations of radioactivity ranged from near backgr...

Preliminary report of the comparison of multiple non-destructive assay techniques on LANL Plutonium Facility waste drums

International Nuclear Information System (INIS)

Bonner, C.; Schanfein, M.; Estep, R.

1999-01-01

Prior to disposal, nuclear waste must be accurately characterized to identify and quantify the radioactive content. The DOE Complex faces the daunting task of measuring nuclear material with both a wide range of masses and matrices. Similarly daunting can be the selection of a non-destructive assay (NDA) technique(s) to efficiently perform the quantitative assay over the entire waste population. In fulfilling its role of a DOE Defense Programs nuclear User Facility/Technology Development Center, the Los Alamos National Laboratory Plutonium Facility recently tested three commercially built and owned, mobile nondestructive assay (NDA) systems with special nuclear materials (SNM). Two independent commercial companies financed the testing of their three mobile NDA systems at the site. Contained within a single trailer is Canberra Industries segmented gamma scanner/waste assay system (SGS/WAS) and neutron waste drum assay system (WDAS). The third system is a BNFL Instruments Inc. (formerly known as Pajarito Scientific Corporation) differential die-away imaging passive/active neutron (IPAN) counter. In an effort to increase the value of this comparison, additional NDA techniques at LANL were also used to measure these same drums. These are comprised of three tomographic gamma scanners (one mobile unit and two stationary) and one developmental differential die-away system. Although not certified standards, the authors hope that such a comparison will provide valuable data for those considering these different NDA techniques to measure their waste as well as the developers of the techniques

Settlement process of radioactive dust to the ground inferred from the atmospheric electric field measurement

Science.gov (United States)

Yamauchi, M.; Takeda, M.; Makino, M.; Owada, T.; Miyagi, I.

2012-01-01

Radioactive materials from the accident at Fukushima Dai-ichi nuclear power plant (FNPP) in March 2011 spread over a large area, increasing the atmospheric electric conductivity by their ionizing effect, and reducing the vertical (downward) component of the DC electric field near the ground, or potential gradient (PG). PG data at Kakioka, 150 km away from the FNPP, showed independent changes compared to the radiation dose rate, and a comparison of these data revealed the local dynamics of the radioactive dust. (1) The initial drop of the PG to almost zero during 14-15 March is most likely due to radioactive dust suspended in the air near the ground during cloudy weather. (2) An episode of PG increase to more than 50 V m-1 on 16 March is most likely due to the re-suspension of the radioactive dust from the surface and subsequent removal from Kakioka by the strong wind from the non-contaminated area. (3) Low but finite values of the PG during 16-20 March most likely reflect a reduced amount of radioactive material near the ground after the above wind transported away the majority of the suspended radioactive dust. (4) Very low values of the PG after substantial rain on 20-22 March most likely reflect settlement of the radioactive material by rain-induced fallout. (5) Temporal recovery of daily variations from the end of March to the middle of April with low nighttime fair-weather baseline PG most likely reflects re-suspension of the radioactive dust into the air from the ground and trees, and subsequent transport to the other region or fallout to the ground until late April. (6) Weakening of the daily variation and gradual recovery of the nighttime fair-weather baseline after mid-April suggests a complete settlement of the radioactive material to the ground with partial migration to the subsurface.

Colorimetric detection with aptamer-gold nanoparticle conjugates coupled to an android-based color analysis application for use in the field.

Science.gov (United States)

Smith, Joshua E; Griffin, Daniel K; Leny, Juliann K; Hagen, Joshua A; Chávez, Jorge L; Kelley-Loughnane, Nancy

2014-04-01

The feasibility of using aptamer-gold nanoparticle conjugates (Apt-AuNPs) to design colorimetric assays for in the field detection of small molecules was investigated. An assay to detect cocaine was designed using two clones of a known cocaine-binding aptamer. The assay was based on the AuNPs difference in affinity for single-stranded DNA (non-binding) and double stranded DNA (target bound). In the first assay, a commonly used design was followed, in which the aptamer and target were incubated to allow binding followed by exposure to the AuNPs. Interactions between the non-bound analytes and the AuNPs surface resulted in a number of false positives. The assay was redesigned by incubating the AuNPs and the aptamer prior to target addition to passivate the AuNPs surface. The adsorbed aptamer was able to bind the target while preventing non-specific interactions. The assay was validated with a number of masking and cutting agents and other controlled substances showing minimal false positives. Studies to improve the assay performance in the field were performed, showing that assay activity could be preserved for up to 2 months. To facilitate the assay analysis, an android application for automatic colorimetric characterization was developed. The application was validated by challenging the assay with cocaine standards of different concentrations, and comparing the results to a conventional plate reader, showing outstanding agreement. Finally, the rapid identification of cocaine in mixtures mimicking street samples was demonstrated. This work established that Apt-AuNPs can be used to design robust assays to be used in the field. Copyright © 2013 Elsevier B.V. All rights reserved.

Results of field testing of radioactive waste forms using lysimeters

International Nuclear Information System (INIS)

McConnell, J.W., Jr.; Rogers, R.D.; Jastrow, J.D.; Wickliff, D.S.

1992-01-01

The Field Lysimeter Investigation: Low-Level Waste Data Base Development Program is obtaining informaiton on the performance of radioactive waste in a disposal environment. Waste forms fabricated using ion-exchange resins from EPICOR-II prefilters employed in the cleanup of the Three Mile Island (TMI) Nuclear Power Station are being tested to develop a low-level waste data base and to obtain information on survivability of waste forms in a disposal environment. In this paper, radionuclide releases from waste forms in the first six years of sampling are presented and discussed. Application of lysimeter data to use in performance assessment models is presented. Initial results from use of data in a performance assessment model are discussed

Field experience with a mobile tomographic nondestructive assay system

International Nuclear Information System (INIS)

Prettyman, T.H.; Betts, S.E.; Taggart, D.P.; Estep, R.J.; Nicholas, N.J.; Lucas, M.C.; Harlan, R.A.

1995-01-01

A mobile tomographic gamma-ray scanner (TGS) developed by Los Alamos National Laboratory was recently demonstrated at the Rocky Flats Environmental Technology Site and is currently in use at Los Alamos waste storage areas. The scanner was developed to assay radionuclides in low-level, transuranic, and mixed waste in containers ranging in size from 2 ft 3 boxes to 83-gallon overpacks. The tomographic imaging capability provides a complete correction for source distribution and matrix attenuation effects, enabling accurate assays of Pu-239 and other gamma-ray emitting isotopes. In addition, the system can reliably detect self-absorbing material such as plutonium metal shot, and can correct for bias caused by self-absorption. The system can be quickly configured to execute far-field scans, segmented gamma-ray scans, and a host of intermediate scanning protocols, enabling higher throughput (up to 20 drums per 8-hour shift). In this paper, we will report on the results of field trials of the mobile system at Rocky Flats and Los Alamos. Assay accuracy is confirmed for cases in which TGS assays can be compared with assays (e.g. with calorimetry) of individual packages within the drums. The mobile tomographic technology is expected to considerably reduce characterization costs at DOE production and environmental technology sites

Solute movement observation in the field soils by means of radioactive tracers

International Nuclear Information System (INIS)

Lichner, L.

1986-01-01

The radioactive tracer method is discussed as applied to transfer velocity measurements of solutions in unsaturated soils, its applicability and the criteria for the choice of the tracer. The method is based on measurement of the radioactive tracer velocity in the field and on laboratory determination of the equilibrium distribution coefficients of the tracer and the solute in the same field soil. From these results and from the soil characteristics (porosity, bulk density) the solute transfer velocity in the field soil can be calculated. The results are presented of 131 I - velocity measurements in the loamy soil in the region of water source Cunovo near Bratislava, which equals 9.29x10 -9 m/s, and in the downstream slope of the earth dam Rozgrund near Banska Stiavnica where the velocity of 131 I - near the dam foot was found to be 2.03 - 2.86 times greater than near the top. Results are also presented of 131 I - , [ 60 Co-EDTA] - and 60 Co 2+ velocity measurements in clay-loam soil at the experimental field of the Research Institute of Irrigation in Most near Bratislava. The results are applicable to evaluation of surface damage to embankments and earth dams, to determination of the conservation zone around water sources, and the establishment of the level of ground water pollution from different sources (waste disposal, agriculture, etc.)

High content screening for G protein-coupled receptors using cell-based protein translocation assays

DEFF Research Database (Denmark)

Grånäs, Charlotta; Lundholt, Betina Kerstin; Heydorn, Arne

2005-01-01

G protein-coupled receptors (GPCRs) have been one of the most productive classes of drug targets for several decades, and new technologies for GPCR-based discovery promise to keep this field active for years to come. While molecular screens for GPCR receptor agonist- and antagonist-based drugs...... will continue to be valuable discovery tools, the most exciting developments in the field involve cell-based assays for GPCR function. Some cell-based discovery strategies, such as the use of beta-arrestin as a surrogate marker for GPCR function, have already been reduced to practice, and have been used...... as valuable discovery tools for several years. The application of high content cell-based screening to GPCR discovery has opened up additional possibilities, such as direct tracking of GPCRs, G proteins and other signaling pathway components using intracellular translocation assays. These assays provide...

Remediation Of Radioactive Contaminated Soil in Oil Fields

International Nuclear Information System (INIS)

Taha, A.A.; Hassib, G.M.; Ibrahim, Z.A.

2011-01-01

Radioactive contamination by naturally occurring radioactive materials (NORM) in evaporation pond has been evaluated. At several onshore oil field locations, the produced water is discharged to form artificial lagoons or ponds. Subsequently, the released waters drain to the ground leaving radioactive deposits associated with the soil that eventually require remedial action in accordance with radiation protection principles. The present study aims to investigate the remediation of contaminated soil in some oil fields and in this concern, two scenarios were proposed. The first scenario is studying the feasibility of using soil washing technique (a physical-chemical separation process) for removing radium-226 from the contaminated soil samples collected from an evaporating pond. The size/activity distribution analyses were carried out. The data obtained showed that almost 68 % of the investigated soil was coarse sand (≥ 300 μm), 28 % was medium and fine sand (≤300 μm and (≥75 μm) and only small fraction of 4 % was silt and clay (≤75 μm). A series of mild acids such as HCl and mild NaCl/HCl (chloride washing) were used for washing the investigated soil fractions. The obtained data showed that the coarse fraction ≥ 300 μm can be re mediated below a regulatory level of 1Bq/g. and the radium from this coarse fraction could be easily removed by screening and chloride washing. For the remediation of (≤ 300 μm and (≥ 75 μm soil fractions, a series of mild chloride washing experiments also showed that the chloride base (NaCl/HCl) was found to be potentially useful. However, there was a difficulty in achieving a low radium value in the fine (≥ 75 μm size fractions using chloride washing. The second scenario is to get rid of all contaminated soil and store it in a concrete basin through the program of radiological protection of personnel and environment. Preliminary gamma survey of contaminated soil showed that the significant area of the investigated

Parameter determination in a groundwater field polluted by radioactive pollutant

International Nuclear Information System (INIS)

Sidauruk, P.; Barokah A; Syafalni; Wibagiyo

1998-01-01

The determination of source location and the corresponding parameters in a contaminated groundwater is very important. To be able to predict the distribution of radioactive contaminant in a contaminated field, the knowledge about the source location and the corresponding parameters is a necessity. The model developed in this paper is based on the fact that the relation between the logarithm of the concentration of the radio active contaminant with the squared coordinate is linear. The contaminant transport parameters as well as the a straight line. In other words, the parameters and the source location are determined in a such way that the linear correlation coefficient between the logarithm of the concentration of the radio active contaminant with the squared coordinate is optimized. The developed model is tested with a synthetic data with a satisfactory results. The synthetic data is generated such that can represent the real field. The synthetic data are generated because the real field data is not available. (authors)

Design and Validation Testing of TruckScan to Assay Large Sacks of Fukushima Radioactive Debris on a Truck

Energy Technology Data Exchange (ETDEWEB)

Suzuki, Atsuo [Canberra-Japan (Japan); Bronson, Frazier [Canberra Industries Inc. (United States)

2015-07-01

As a result of the March 2011 earthquake and resulting tsunami in Japan, there was a serious accident at the Fukushima Dai-ichi Nuclear Power Plant. This accident has contaminated soil and vegetation in a wide area around the plant. Decontamination projects over the last 4 years have resulted in large numbers of 1 cubic meter canvas bags of debris, commonly called Super Sacks [SS]. These are currently stored nearby where they were generated, but starting in 2015, they will be moved to various Interim Storage Facilities [ISF]. Trucks will typically carry 8-20 of these SSs. When the trucks arrive at the ISF they need to be rapidly sorted into groups according to radioactivity level, for efficient subsequent processing. Canberra Industries, Inc. [CI] has designed a new truck monitoring system 'TruckScan' for use at these ISFs. The TruckScan system must measure the entire truck loaded with multiple closely packed SSs, and generate a nuclide specific assay report showing the radioactivity in each individual SS. The Canberra-Japan office, along with Obayashi Corporation have performed validation testing to demonstrate to the regulatory authorities that the proposed technique was sufficiently accurate. These validation tests were conducted at a temporary storage area in Fukushima prefecture. Decontaminated waste of various representative types and of various levels of radioactivity was gathered and mixed to create homogeneous volumes. These volumes were sampled multiple times and assayed with laboratory HPGe detectors to determine the reference concentration of each pile. Multiple SSs were loaded from each pile. Some of the SSs were filled 50% full, others 75% full, and others 100% full, to represent the typical loading configuration of the existing SSs in the field. The content of the SSs are either sand, soil, or vegetation with densities ranging from 0.3 g/cc - 1.6 g/cc. These SSs with known concentrations of Cs-134 and Cs-137 were then loaded onto trucks in

Design and Validation Testing of TruckScan to Assay Large Sacks of Fukushima Radioactive Debris on a Truck

International Nuclear Information System (INIS)

Suzuki, Atsuo; Bronson, Frazier

2015-01-01

As a result of the March 2011 earthquake and resulting tsunami in Japan, there was a serious accident at the Fukushima Dai-ichi Nuclear Power Plant. This accident has contaminated soil and vegetation in a wide area around the plant. Decontamination projects over the last 4 years have resulted in large numbers of 1 cubic meter canvas bags of debris, commonly called Super Sacks [SS]. These are currently stored nearby where they were generated, but starting in 2015, they will be moved to various Interim Storage Facilities [ISF]. Trucks will typically carry 8-20 of these SSs. When the trucks arrive at the ISF they need to be rapidly sorted into groups according to radioactivity level, for efficient subsequent processing. Canberra Industries, Inc. [CI] has designed a new truck monitoring system 'TruckScan' for use at these ISFs. The TruckScan system must measure the entire truck loaded with multiple closely packed SSs, and generate a nuclide specific assay report showing the radioactivity in each individual SS. The Canberra-Japan office, along with Obayashi Corporation have performed validation testing to demonstrate to the regulatory authorities that the proposed technique was sufficiently accurate. These validation tests were conducted at a temporary storage area in Fukushima prefecture. Decontaminated waste of various representative types and of various levels of radioactivity was gathered and mixed to create homogeneous volumes. These volumes were sampled multiple times and assayed with laboratory HPGe detectors to determine the reference concentration of each pile. Multiple SSs were loaded from each pile. Some of the SSs were filled 50% full, others 75% full, and others 100% full, to represent the typical loading configuration of the existing SSs in the field. The content of the SSs are either sand, soil, or vegetation with densities ranging from 0.3 g/cc - 1.6 g/cc. These SSs with known concentrations of Cs-134 and Cs-137 were then loaded onto trucks in

Radioactive aerosol detection station for near real-time atmospheric monitoring

International Nuclear Information System (INIS)

Mason, L.R.; Bohner, John D.

1997-01-01

A radionuclide aerosol detection station has been developed to measure radioactivity in the environment. The objective is to monitor the atmosphere for anthropogenic radioactivity that could be indicative of nuclear weapons tests to verify the Comprehensive Nuclear Test Ban Treaty. Eighty stations will form the backbone of the International Monitoring System in which stations are linked to a central analysis facility called the International Data Centre. Data are transmitted to this centre in near real-time to facilitate rapid detection. Principal process of the field measurement are collection, separation, and assay. Collection of airborne radioactivity is achieved through high-volume air sampling. Aerosols separation is accomplished by high-efficiency particulate filtration. Radionuclides assay is achieved by in-situ high resolution gamma spectrometry. These modules are integrated into a unit that provides power, control, and communication support subsystems. Station operation is semi-automatic requiring only minimal human interaction. (author). 6 refs., 3 figs., 3 tabs

Metrological tests of a 200 L calibration source for HPGE detector systems for assay of radioactive waste drums.

Science.gov (United States)

Boshkova, T; Mitev, K

2016-03-01

In this work we present test procedures, approval criteria and results from two metrological inspections of a certified large volume (152)Eu source (drum about 200L) intended for calibration of HPGe gamma assay systems used for activity measurement of radioactive waste drums. The aim of the inspections was to prove the stability of the calibration source during its working life. The large volume source was designed and produced in 2007. It consists of 448 identical sealed radioactive sources (modules) apportioned in 32 transparent plastic tubes which were placed in a wooden matrix which filled the drum. During the inspections the modules were subjected to tests for verification of their certified characteristics. The results show a perfect compliance with the NIST basic guidelines for the properties of a radioactive certified reference material (CRM) and demonstrate the stability of the large volume CRM-drum after 7 years of operation. Copyright © 2015 Elsevier Ltd. All rights reserved.

A novel multiparametric flow cytometry-based cytotoxicity assay simultaneously immunophenotypes effector cells: comparisons to a 4 h 51Cr-release assay.

Science.gov (United States)

Kim, G G; Donnenberg, V S; Donnenberg, A D; Gooding, W; Whiteside, T L

2007-08-31

Natural killer (NK) cell-or T cell-mediated cytotoxicity traditionally is measured in 4-16 h (51)Cr-release assays (CRA). A new four-color flow cytometry-based cytotoxicity assay (FCC) was developed to simultaneously measure NK cell cytotoxicity and NK cell phenotype (CD3(-)CD16(+)CD56(+)). Target cells, K562 or Daudi, were labeled with Cell Tracker Orange (CTO) prior to the addition of effector cells. Following co-incubation, 7 amino-actinomycin D (7-AAD) was added to measure death of target cells. The phenotype of effectors, viability of targets, the formation of tumor-effector cell conjugates and absolute numbers of all cells were measured based on light scatter (FSC/SSC), double discrimination of the fluorescence peak integral and height, and fluorescence intensity. Kinetic studies (0.5 and 1 to 4 h) at different effector to target (E:T) cell ratios (50, 25, 12, and 6) confirmed that the 3 h incubation was optimal. The FCC assay is more sensitive than the CRA, has a coefficient of variation (CV) 8-13% and reliably measures NK cell-or lymphokine-activated killer (LAK) cell-mediated killing of target cells in normal controls and subjects with cancer. The FCC assay can be used to study a range of phenotypic attributes, in addition to lytic activity of various subsets of effector cells, without radioactive tracers and thus, it is relatively inexpensive. The FCC assay has a potential for providing information about molecular interactions underlying target cell lysis and thus becoming a major tool for studies of disease pathogenesis as well as development of novel immune therapies.

Skin sensitization potency and cross-reactivity of p-phenylenediamine and its derivatives evaluated by non-radioactive murine local lymph node assay and guinea-pig maximization test.

Science.gov (United States)

Yamano, Tetsuo; Shimizu, Mitsuru

2009-04-01

p-Phenylenediamine (PPD)-related chemicals have been used as antioxidants in rubber products, and many cases of contact dermatitis caused by these chemicals have been reported. The aim of this study was to investigate relative sensitizing potency and cross-reactivity among PPD derivatives. Five PPD derivatives, p-aminodiphenylamine (PADPA), N,N'-diphenyl-p-phenylenediamine (DPPD), N-isopropyl-N'-phenyl-p-phenylenediamine (IPPD), N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (DMBPPD), N-(1-methylheptyl)-N'-phenyl-p-phenylenediamine (MHPPD), and the core chemical PPD were evaluated for their sensitizing potency and cross-reactivity using the non-radioactive murine local lymph node assay (LLNA) and the guinea-pig maximization test (GPMT). PPD and all the derivatives were identified as primary sensitizers in both tests. The order of potency in the LLNA was as follows: IPPD and PADPA > PPD > DMBPPD and MHPPD > DPPD. In the GPMT, all six groups of animals sensitized with one of these chemicals cross-reacted to four other derivatives. Specifically, the five groups that have a common basic PADPA structure, that is PADPA, DPPD, IPPD, DMBPPD, and MHPPD, all reacted to each other at almost the same scores, while none of them reacted to PPD. The cross-reactivity profile found in the study was to some extent different from that in previous human data, where distinction between cross-reaction and concomitant primary sensitization is not always clear.

Quantitative comparison of the results obtained by the multiple-dose guinea pig maximization test and the non-radioactive murine local lymph-node assay for various biocides.

Science.gov (United States)

Yamano, Tetsuo; Shimizu, Mitsuru; Noda, Tsutomu

2005-07-01

We compared the results of the multiple-dose guinea pig maximization test (GPMT) and the non-radioactive murine local lymph-node assay (LLNA) for various biocides. Thirteen out of 17 positive biocides in the GPMT gave positive results in the LLNA. In the GPMT, the minimum first induction doses ranged over four orders (0.00005-0.5%), while elicitation-threshold doses, which were evaluated using an optimally sensitized group of animals in the multiple-dose studies, ranged over five orders (0.00006-2.8%). In the LLNA, minimum induction doses ranged over more than three orders (0.01-30%). With respect to 13 biocides that were positive in both the GPMT and the LLNA, results were quantitatively compared. When compared after conversion to corresponding area doses (microg/cm), the minimum doses required to elicit skin reaction in guinea pigs were always lower than that for induction in mice with all biocides. Correlation between minimum induction doses from the GPMT and the LLNA seemed poor (r=0.57), while that between minimum induction doses in the LLNA and elicitation-threshold doses in the GPMT was relatively good (r=0.73). The results suggest the possibility to estimate human elicitation-threshold doses, which are definitely lacking in the process of risk assessment for skin-sensitizers, from the data of the LLNA.

Quantitative comparison of the results obtained by the multiple-dose guinea pig maximization test and the non-radioactive murine local lymph-node assay for various biocides

International Nuclear Information System (INIS)

Yamano, Tetsuo; Shimizu, Mitsuru; Noda, Tsutomu

2005-01-01

We compared the results of the multiple-dose guinea pig maximization test (GPMT) and the non-radioactive murine local lymph-node assay (LLNA) for various biocides. Thirteen out of 17 positive biocides in the GPMT gave positive results in the LLNA. In the GPMT, the minimum first induction doses ranged over four orders (0.00005-0.5%), while elicitation-threshold doses, which were evaluated using an optimally sensitized group of animals in the multiple-dose studies, ranged over five orders (0.00006-2.8%). In the LLNA, minimum induction doses ranged over more than three orders (0.01-30%). With respect to 13 biocides that were positive in both the GPMT and the LLNA, results were quantitatively compared. When compared after conversion to corresponding area doses (μg/cm), the minimum doses required to elicit skin reaction in guinea pigs were always lower than that for induction in mice with all biocides. Correlation between minimum induction doses from the GPMT and the LLNA seemed poor (r = 0.57), while that between minimum induction doses in the LLNA and elicitation-threshold doses in the GPMT was relatively good (r = 0.73). The results suggest the possibility to estimate human elicitation-threshold doses, which are definitely lacking in the process of risk assessment for skin-sensitizers, from the data of the LLNA

Radioactivity Measurements on Glazed Ceramic Surfaces.

Science.gov (United States)

Hobbs, T G

2000-01-01

A variety of commonly available household and industrial ceramic items and some specialty glass materials were assayed by alpha pulse counting and ion chamber voltage measurements for radioactivity concentrations. Identification of radionuclides in some of the items was performed by gamma spectroscopy. The samples included tableware, construction tiles and decorative tiles, figurines, and other products with a clay based composition. The concentrations of radioactivity ranged from near background to about four orders of magnitude higher. Almost every nuclide identification test demonstrated some radioactivity content from one or more of the naturally occurring radionuclide series of thorium or uranium. The glazes seemed to contribute most of the activity, although a sample of unglazed pottery greenware showed some activity. Samples of glazing paints and samples of deliberately doped glass from the World War II era were included in the test, as was a section of foam filled poster board. A glass disc with known (232)Th radioactivity concentration was cast for use as a calibration source. The results from the two assay methods are compared, and a projection of sensitivity from larger electret ion chamber devices is presented.

Settlement process of radioactive dust to the ground inferred from the atmospheric electric field measurement

Directory of Open Access Journals (Sweden)

M. Yamauchi

2012-01-01

Full Text Available Radioactive materials from the accident at Fukushima Dai-ichi nuclear power plant (FNPP in March 2011 spread over a large area, increasing the atmospheric electric conductivity by their ionizing effect, and reducing the vertical (downward component of the DC electric field near the ground, or potential gradient (PG. PG data at Kakioka, 150 km away from the FNPP, showed independent changes compared to the radiation dose rate, and a comparison of these data revealed the local dynamics of the radioactive dust. (1 The initial drop of the PG to almost zero during 14–15 March is most likely due to radioactive dust suspended in the air near the ground during cloudy weather. (2 An episode of PG increase to more than 50 V m−1 on 16 March is most likely due to the re-suspension of the radioactive dust from the surface and subsequent removal from Kakioka by the strong wind from the non-contaminated area. (3 Low but finite values of the PG during 16–20 March most likely reflect a reduced amount of radioactive material near the ground after the above wind transported away the majority of the suspended radioactive dust. (4 Very low values of the PG after substantial rain on 20–22 March most likely reflect settlement of the radioactive material by rain-induced fallout. (5 Temporal recovery of daily variations from the end of March to the middle of April with low nighttime fair-weather baseline PG most likely reflects re-suspension of the radioactive dust into the air from the ground and trees, and subsequent transport to the other region or fallout to the ground until late April. (6 Weakening of the daily variation and gradual recovery of the nighttime fair-weather baseline after mid-April suggests a complete settlement of the radioactive material to the ground with partial migration to the subsurface.

In-situ measurement of environment radioactivity by mobile nuclear field laboratory (MNFL)

International Nuclear Information System (INIS)

Gopalani, Deepak; Mathur, A.P.; Rawat, D.K.; Barala, S.S.; Singhal, K.P.; Singh, G.P.; Samant, R.P.

2008-01-01

In-situ measurement of environment radioactivity is useful tool for determine the unusual increase of radioactivity at any place due to any nuclear eventuality take place. A mobile nuclear field laboratory has been designed and developed for in-situ measurement of environment radioactivity at any desired location. This vehicle is equipped with different monitoring and analysis instruments. These equipment can be operated while vehicle is moving. The measured data can be stored in computer. This vehicle has the space for storage of various environmental matrices of affected area and these can analysis in laboratory. (author)

Compliance assurance in the field of radioactive material transport in Russia

International Nuclear Information System (INIS)

Ershov, V.; Syssoev, M.

1999-01-01

The main provisions of the system of compliance assurance, as understood in the IAEA Safety Regulations, are presented in this article as they are applied in Russia in the field of transport of radioactive materials. The urgency of the development and enactment of the uniform programme of compliance assurance in this area is underlined since it is foreseen by the new national regulations for the safety of radioactive material transport in Russia. (author)

Radioactive Plumes Monitoring Simulator

International Nuclear Information System (INIS)

Kapelushnik, I.; Sheinfeld, M.; Avida, R.; Kadmon, Y.; Ellenbogen, M.; Tirosh, D.

1999-01-01

The Airborne Radiation Monitoring System (ARMS) monitors air or ground radioactive contamination. The contamination source can be a radioactive plume or an area contaminated with radionuclides. The system is based on two major parts, an airborne unit carried by a helicopter and a ground station carried by a truck. The system enables real time measurement and analysis of radioactive plumes as well as post flight processing. The Radioactive Plumes Monitoring Simulator purpose is to create a virtual space where the trained operators experience full radiation field conditions, without real radiation hazard. The ARMS is based on a flying platform and hence the simulator allows a significant reduction of flight time costs

A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer.

Science.gov (United States)

Altevogt, Dominik; Hrenn, Andrea; Kern, Claudia; Clima, Lilia; Bannwarth, Willi; Merfort, Irmgard

2009-10-07

Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

A fluorescence sedimentation assay for dsDNA antibodies

DEFF Research Database (Denmark)

Duus, K; Draborg, A H; Güven, E

2017-01-01

The Farr assay is a radioimmunoassay (RIA) for dsDNA antibodies, based on antibody precipitation using ammonium sulphate and quantification using radio-labelled dsDNA. The RIA-Farr assay offers outstanding clinical specificity and sensitivity for systemic lupus erythematosus (SLE) compared to other...... on precipitation with polyethylene glycol (PEG) and fluorescence of EvaGreen intercalated in dsDNA as detection principle. As dsDNA antibodies are quantified using fluorescence, the disadvantages of working with radioactivity are eliminated. The Fluoro-Farr assay was developed and validated, and the diagnostic...

Report of the consultants' meeting on radioimmunometric assays for non-clinical applications

International Nuclear Information System (INIS)

2000-01-01

A meeting between three experts and various Agency staff with specific areas of expertise was convened to review present status of radiolabelled immunoassays i.e. radioimmunoassay (RIA) and immunradiometric assay (IRMA) and their relevance to non- clinical applications. For many years, the Agency has been active in supporting the devolution of RIA and IRMA expertise in clinical applications, particularly in the field of thyroid disease and its management. The Agency's strategy was based on a perceived need to reduce the high cost of imported reagents and the poor working practices that this encouraged. This has been achieved through the use of bulk reagents (rather than 'kits') and the transfer of existing technology. The success of the Agency's approach is evident from the high degree of self- sufficiency now available in many developing countries. In recent years, the increasing workload for clinical analyses has necessitated an increase in automation, which in turn has required a change to more non-isotopic alternatives. This change seen in the more developed countries, and beginning to be introduced elsewhere, is likely lo continue. However, RIA/IRMA will continue to be used in many parts of the world for some time to come. In recent years there has been an increasing interest in the use of immunoassay technology in non-clinical applications, e.g., environmental monitoring, quality control in food production and livestock management. RIA and IRMA continue to play a major role in research

Transport of radioactive materials

International Nuclear Information System (INIS)

1988-07-01

The norm which establishes the requirements of radiation protection and safety related to the transport of radioactive materials, aiming to keep a suitable control level of eventual exposure of personnels, materials and environment of ionizing radiation, including: specifications on radioactive materials for transport, selection of package type; specification of requirements of the design and assays of acceptance of packages; disposal related to the transport; and liability and administrative requirements, are presented. This norm is applied to: truckage, water carriage and air service; design, fabrication, assays and mantenaince of packages; preparation, despatching, handling, loading storage in transition and reception in the ultimate storage of packages; and transport of void packages which have been contained radioactive materials. (M.C.K.) [pt

Radioactive waste processing field

International Nuclear Information System (INIS)

Ito, Minoru.

1993-01-01

Storing space for radioactive wastes (storage tunnels) are formed underground of the sea bottom along coast. A plurality of boreholes through which sea water flows are pored vertically in a direction intersecting underground streams of brine in the ground between the tunnels and seaside. Sea water introduction pipes are joined to the upper side walls of the boreholes. The sea water introduction pipes have introduction ports protruded under the sea level of the coastal sea area region. Since sea water flows from the introduction ports to the boreholes passing through the sea water introduction pipes, sea water is always filled in the boreholes. Therefore, brine is sufficiently supplied toward the land by sea water from the boreholes, the underground stream of brine is negligibly small. This can prevent radioactive contamination due to flow of the underground water when radioactive wastes are buried in the underground near coast. (I.N.)

Pollution by radioactive iodine and radioactive cesium in fruit fields

International Nuclear Information System (INIS)

Ohtsuki, Tsutomu; Kikunaga, Hidetoshi; Izumi, Yuichi

2011-01-01

A lot of radioactive materials were spread out by the accident of the Fukushima Daiichi nuclear power station caused by the tsunami accompanying earthquake in the area of Tohoku district-Pacific Ocean, in 2011/3/11. In early stage, short half-life nuclides, such as "1"3"2Te, "1"3"1I, "1"3"6Cs, as well as "1"3"4Cs, "1"3"7Cs were observed in fields. Now "1"3"4Cs and "1"3"7Cs which are comparatively long half-life became dominant observing nuclides. The nuclear power plant accident brought serious damages in many directions, especially, the influence of the field pollution by these nuclides is great in the agriculture, and the quick actions and the measures are needed. The influence of "1"3"4Cs and "1"3"7Cs to the fruit tree were investigated in cooperation with Fukushima agricultural center, Institute of Fruit Tree Science. We report several facts of the transfer and translocation mechanism of "1"3"4Cs and "1"3"7Cs in fruit trees. (author)

TRU waste-assay instrumentation and application in nuclear-facility decommissioning

International Nuclear Information System (INIS)

Umbarger, C.J.

1982-01-01

The Los Alamos TRU waste assay program is developing measurement techniques for TRU and other radioactive waste materials generated by the nuclear industry, including decommissioning programs. Systems are now being fielded for test and evaluation purposes at DOE TRU waste generators. The transfer of this technology to other facilities and the commercial instrumentation sector is well in progress. 6 figures

Requirements for a radioactive waste data base

International Nuclear Information System (INIS)

Sato, Y.; Kobayashi, I.; Kikuchi, M.

1990-01-01

With the progress of nuclear fuel cycle in Japan, various types of radioactive waste will generate at each nuclear facility in the cycle. Therefor generated volume and stored quantity of waste will be supposed to increase. From the viewpoints of safety and public acceptance, by using mainframe computer it is necessary that the treatment of historical waste data, the estimation of generated waste volume and stored quantity and the investigation of research and development status of waste processing and disposal are carried out. This paper proposes design and development of the radioactive waste data base which is able to properly and correctly manage and grasp numerical and/or documentary information for generated radioactive waste. So the data base will be expected to use for planning the future management of radioactive waste. (author)

Development of system for management of radioactive waste from non-nuclear application in Russia

International Nuclear Information System (INIS)

Barinov, A.

2000-01-01

The 'Radon' system serves for collecting, transporting, conditioning and disposal of radioactive waste with low and intermediate level activity and spent ionizing sources. The technical policy in this field is embodied most completely in the 'Concept of RF Minatom on radioactive waste management', which outlines the activities till 2025. The main organizational and technical measures in enhancing safety in the process of radioactive waste management as well as the organization of radiation control are described. The main statements of the quality assurance programme for the radioactive waste management are presented

Development of system for management of radioactive waste from non-nuclear application in Russia

Energy Technology Data Exchange (ETDEWEB)

Barinov, A [SIA ' Radon' , Moskow (Russian Federation)

2000-07-01

The 'Radon' system serves for collecting, transporting, conditioning and disposal of radioactive waste with low and intermediate level activity and spent ionizing sources. The technical policy in this field is embodied most completely in the 'Concept of RF Minatom on radioactive waste management', which outlines the activities till 2025. The main organizational and technical measures in enhancing safety in the process of radioactive waste management as well as the organization of radiation control are described. The main statements of the quality assurance programme for the radioactive waste management are presented.

The Belgian approach and status on the radiological surveillance of radioactive substances in metal scrap and non-radioactive waste and the financing of orphan sources

International Nuclear Information System (INIS)

Braeckeveldt, Marnix; Preter, Peter De; Michiels, Jan; Pepin, Stephane; Schrauben, Manfred; Wertelaers, An

2007-01-01

Numerous facilities in the non-nuclear sector in Belgium (e.g. in the non-radioactive waste processing and management sector and in the metal recycling sector) have been equipped with measuring ports for detecting radioactive substances. These measuring ports prevent radioactive sources or radioactive contamination from ending up in the material fluxes treated by the sectors concerned. They thus play an important part in the protection of the workers and the people living in the neighbourhood of the facilities, as well as in the protection of the population and the environment in general. In 2006, Belgium's federal nuclear control agency (FANC/AFCN) drew up guidelines for the operators of non-nuclear facilities with a measuring port for detecting radioactive substances. These guidelines describe the steps to be followed by the operators when the port's alarm goes off. Following the publication of the European guideline 2003/122/EURATOM of 22 December 2003 on the control of high-activity sealed radioactive sources and orphan sources, a procedure has been drawn up by FANC/AFCN and ONDRAF/NIRAS, the Belgian National Agency for Radioactive Waste and Enriched Fissile Materials, to identify the responsible to cover the costs relating to the further management of detected sealed sources and if not found to declare the sealed source as an orphan source. In this latter case and from mid-2006 the insolvency fund managed by ONDRAF/NIRAS covers the cost of radioactive waste management. At the request of the Belgian government, a financing proposal for the management of unsealed orphan sources as radioactive waste was also established by FANC/AFCN and ONDRAF/NIRAS. This proposal applies the same approach as for sealed sources and thus the financing of unsealed orphan sources will also be covered by the insolvency fund. (authors)

Advantages of the segregation step for the radioactive waste management

International Nuclear Information System (INIS)

Medeiros, Regina Bitelli; Mattos, Maria Fernanda S.S.

2002-01-01

Due to the increasing use of radioactive materials in the research activities, the waste management is essential to guarantee personnel safety and the preservation of the environmental quality. It is possible to determine the date of discharge in the public sewage treatment system based on the estimated activity of the radioactive waste and on the solid waste discharge limit of 74 Bq/g as recommended by the radiation protection rules. The goal of this work is to demonstrate the advantages of the waste segregation by specific activity as a means of minimization of the stored waste. The residual specific activity and volume were estimated in the several steps of assays using 32 P and 3 H. The storage times were calculated and compared with the estimated time considering the residual activity as 2 % of the total activity used in the experiment. The segregation by steps of the assay allowed for the reduction of the waste volume and stored time. In the assays with 3 H only 20 % of the total waste generated was stored and in the assays with 32 P it was possible to discharge 90 % of the radioactive waste after 38 days. (author)

Management of radioactive wastes from non-power applications. The Cuban experience

International Nuclear Information System (INIS)

Benitez, J.C.; Salgado, M.; Jova, L.

2001-01-01

Full text: Origin of Radioactive Wastes. The wastes arisen from the applications of radioisotopes in medicine are mainly liquids and solid materials contaminated with short lived radionuclides and sealed sources used in radiotherapy and for sterilization of medical materials. Radioactive wastes from industrial applications are generally disused sealed sources used in level detection, quality control, smoke detection and non-destructive testing. The principal forms of wastes generated by research institutes are miscellaneous liquids, trash, biological wastes, and scintillation vials, sealed sources and targets. Solid radioactive wastes are mainly produced during research works, cleaning and decontamination activities and they consist of rags, paper, cellulose, plastics, gloves, clothing, overshoes, etc. Laboratory materials such as cans, polyethylene bags and glass bottles also contribute to the solid waste inventory. Small quantities of non-compactable wastes are also collected and received for treatment. They include wood pieces, metal scrap, defective components and tools. Radioactive Waste Management Policy and Infrastructure. Since 1994 the Cuban integral policy of nuclear development is entrusted to the Nuclear Energy Agency of the Ministry of Science, Technology and Environment (CITMA). The National Center for Nuclear Safety (CNSN) is responsible for the licensing and supervision of radioactive and nuclear installations. The CPHR is in charge of waste management policy and therefore is responsible for centralized collection, transportation, treatment, conditioning, long term storage, and disposal of radioactive waste, as well as for developing new waste conditioning and containment methods. Radioactive Waste Management Facilities. Waste Treatment and Conditioning Plant (WTCP). The present facility is a building that includes a technological area of 100 m 2 and a laboratory area with a surface of around 30 m 2 . Other areas to be distinguished inside the

Study on a new calibration methods of in-situ HPGe γ spectrometers used for non-destructive analyzing radioactivity in nuclear facilities decommissioning

International Nuclear Information System (INIS)

Xiao Xuefu; Song Lijun; Wang Yulai; Wen Fuping; Liao Haitao; Ban Ying; Xia Yihua; Li Ruixiang; Li Hang; Tu Xingmin

2007-06-01

A new calibration technique, which is the Monte Carlo modeling technique, of in-situ HPGe γ spectrometers used for non-destructive analyzing radioactivity in nuclear facilities decommissioning, is presented. A series of assay for some stainless steel pipes and tanks in some nuclear facilities/laboratories of CIAE are taken on site with the in-situ HPGe γ spectrometer. At the same time, some examples are taken and analyzed in laboratories. The relative bias/variation between the values of activity measured by in-situ HPGe γ spectrometers on site and that analyzed in laboratory is less than ±45.0%. (authors)

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells.

Science.gov (United States)

Casanova, Alain; Low, Shyan H; Emmenlauer, Mario; Conde-Alvarez, Raquel; Salcedo, Suzana P; Gorvel, Jean-Pierre; Dehio, Christoph

2016-08-05

Brucella species are facultative intracellular pathogens that infect animals as their natural hosts. Transmission to humans is most commonly caused by direct contact with infected animals or by ingestion of contaminated food and can lead to severe chronic infections. Brucella can invade professional and non-professional phagocytic cells and replicates within endoplasmic reticulum (ER)-derived vacuoles. The host factors required for Brucella entry into host cells, avoidance of lysosomal degradation, and replication in the ER-like compartment remain largely unknown. Here we describe two assays to identify host factors involved in Brucella entry and replication in HeLa cells. The protocols describe the use of RNA interference, while alternative screening methods could be applied. The assays are based on the detection of fluorescently labeled bacteria in fluorescently labeled host cells using automated wide-field microscopy. The fluorescent images are analyzed using a standardized image analysis pipeline in CellProfiler which allows single cell-based infection scoring. In the endpoint assay, intracellular replication is measured two days after infection. This allows bacteria to traffic to their replicative niche where proliferation is initiated around 12 hr after bacterial entry. Brucella which have successfully established an intracellular niche will thus have strongly proliferated inside host cells. Since intracellular bacteria will greatly outnumber individual extracellular or intracellular non-replicative bacteria, a strain constitutively expressing GFP can be used. The strong GFP signal is then used to identify infected cells. In contrast, for the entry assay it is essential to differentiate between intracellular and extracellular bacteria. Here, a strain encoding for a tetracycline-inducible GFP is used. Induction of GFP with simultaneous inactivation of extracellular bacteria by gentamicin enables the differentiation between intracellular and extracellular

European Community legislation and recommendations in the field of radioactive waste management and disposal

International Nuclear Information System (INIS)

Schaller, K.H.; Orlowski, S.

1993-01-01

Radiation protection principles are the foundation for national and European Community legislation and recommendations in the field of management and disposal of radioactive waste. Directives set up at Community level, which are to be implemented in the national legislative framework of the Member States are summarized. Policies and strategies in radioactive waste management are of national competence, as well as ensuring safety of all steps of handling, storage and final disposal; recommendations at Community level are developed to assist national authorities and agencies in determining their strategy. Existing recommendations are described and a preview of recommendations under preparation is given. Legislation in this field is mainly aimed at radioactive waste arising from operation and decommissioning of nuclear installations; for radioactive waste produced outside the nuclear fuel cycle and for material with significant levels of naturally occurring radionuclides special situations exist, and harmonization of legislation and the development of common practices on the management of such wastes may be desirable

Scoping study to expedite development of a field deployable and portable instrument for UF6 enrichment assay

Energy Technology Data Exchange (ETDEWEB)

Chan, George; Valentine, John D.; Russo, Richard E.

2017-09-14

The primary objective of the present study is to identity the most promising, viable technologies that are likely to culminate in an expedited development of the next-generation, field-deployable instrument for providing rapid, accurate, and precise enrichment assay of uranium hexafluoride (UF6). UF6 is typically involved, and is arguably the most important uranium compound, in uranium enrichment processes. As the first line of defense against proliferation, accurate analytical techniques to determine the uranium isotopic distribution in UF6 are critical for materials verification, accounting, and safeguards at enrichment plants. As nuclear fuel cycle technology becomes more prevalent around the world, international nuclear safeguards and interest in UF6 enrichment assay has been growing. At present, laboratory-based mass spectrometry (MS), which offers the highest attainable analytical accuracy and precision, is the technique of choice for the analysis of stable and long-lived isotopes. Currently, the International Atomic Energy Agency (IAEA) monitors the production of enriched UF6 at declared facilities by collecting a small amount (between 1 to 10 g) of gaseous UF6 into a sample bottle, which is then shipped under chain of custody to a central laboratory (IAEA’s Nuclear Materials Analysis Laboratory) for high-precision isotopic assay by MS. The logistics are cumbersome and new shipping regulations are making it more difficult to transport UF6. Furthermore, the analysis is costly, and results are not available for some time after sample collection. Hence, the IAEA is challenged to develop effective safeguards approaches at enrichment plants. In-field isotopic analysis of UF6 has the potential to substantially reduce the time, logistics and expense of sample handling. However, current laboratory-based MS techniques require too much infrastructure and operator expertise for field deployment and operation. As outlined in the IAEA Department of Safeguards Long

Colloid Facilitated Transport of Radioactive Cations in the Vadose Zone: Field Experiments Oak Ridge

Energy Technology Data Exchange (ETDEWEB)

James E. Saiers

2012-09-20

The overarching goal of this study was to improve understanding of colloid-facilitated transport of radioactive cations through unsaturated soils and sediments. We conducted a suite of laboratory experiments and field experiments on the vadose-zone transport of colloids, organic matter, and associated contaminants of interest to the U.S. Department of Energy (DOE). The laboratory and field experiments, together with transport modeling, were designed to accomplish the following detailed objectives: 1. Evaluation of the relative importance of inorganic colloids and organic matter to the facilitation of radioactive cation transport in the vadose zone; 2. Assessment of the role of adsorption and desorption kinetics in the facilitated transport of radioactive cations in the vadose zone; 3. Examination of the effects of rainfall and infiltration dynamics and in the facilitated transport of radioactive cations through the vadose zone; 4. Exploration of the role of soil heterogeneity and preferential flow paths (e.g., macropores) on the facilitated transport of radioactive cations in the vadose zone; 5. Development of a mathematical model of facilitated transport of contaminants in the vadose zone that accurately incorporates pore-scale and column-scale processes with the practicality of predicting transport with readily available parameters.

Immunophenotyping does not improve predictivity of the local lymph node assay in mice.

Science.gov (United States)

Strauss, Volker; Kolle, Susanne N; Honarvar, Naveed; Dammann, Martina; Groeters, Sibylle; Faulhammer, Frank; Landsiedel, Robert; van Ravenzwaay, Bennard

2015-04-01

The local lymph node assay (LLNA) is a regulatory accepted test for the identification of skin sensitizing substances by measuring radioactive thymidine incorporation into the lymph node. However, there is evidence that LLNA is overestimating the sensitization potential of certain substance classes in particular those exerting skin irritation. Some reports describe the additional use of flow cytometry-based immunophenotyping to better discriminate irritants from sensitizing irritants in LLNA. In the present study, the 22 performance standards plus 8 surfactants were assessed using the radioactive LLNA method. In addition, lymph node cells were immunophenotyped to evaluate the specificity of the lymph node response using cell surface markers such as B220 or CD19, CD3, CD4, CD8, I-A(κ) and CD69 with the aim to allow a better discrimination above all between irritants and sensitizers, but also non-irritating sensitizers and non-sensitizers. However, the markers assessed in this study do not sufficiently differentiate between irritants and irritant sensitizers and therefore did not improve the predictive capacity of the LLNA. Copyright © 2014 John Wiley & Sons, Ltd.

Measurability of non-abelium gauge fields

Energy Technology Data Exchange (ETDEWEB)

Ivanenko, D.D.; Obukhov, Yu.N.

New estimations of the accuracy of measurement of non-abeliar gauge field components are obtained on the base of qualitative analysis of the test body equations of motion. They generalize the Bohr and Rosenfeld results on the measurability of an electomagnetic field for the case of an arbitrary gauge group.

An ultrafiltration assay for lysyl oxidase

International Nuclear Information System (INIS)

Shackleton, D.R.; Hulmes, D.J.

1990-01-01

A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Enzyme activity is determined by scintillation counting of the ultrafiltrate, after subtraction of radioactivity released in the presence of beta-aminopropionitrile, a specific inhibitor of the enzyme. Conditions are described which optimize both the sensitivity and the efficient use of substrate. The assay shows linear inhibition of activity in up to 1 M urea; hence, as the enzyme is normally diluted in the assay, samples in 6 M urea can be assayed directly, without prior dialysis, and corrected for partial inhibition. Comparable results are obtained when enzyme activity is assayed by ultrafiltration or microdistillation. The assay is simple and convenient and, by using disposable containers throughout, it eliminates the need for time-consuming decontamination of radioactive glassware

Microbead agglutination based assays

KAUST Repository

Kodzius, Rimantas; Castro, David; Foulds, Ian G.; Parameswaran, Ash M.; Sumanpreet, K. Chhina

2013-01-01

We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling

Challenges of Non-Destructive Assay Waste Measurement

International Nuclear Information System (INIS)

Shull, A.H.

2003-01-01

Historically, the Savannah River Site (SRS) routinely produced special nuclear material (SNM), which provided stable measurement conditions for the non-destructive assay (NDA) methods. However, the main mission of SRS has changed from the production of SNM to the processing of waste and material stabilization. Currently, the purpose of processing is to recover the SNM from the waste and stabilization materials, much of which is from other DOE facilities. These missions are usually of a short duration, but require non-destructive assay (NDA) accountability measurements on materials of varying composition and geometric configuration. These missions usually have cost and time constraints, which sometimes require re-application of existing NDA methods to waste measurements. Usually, each new material or re-application of the NDA method to a different SNM campaign requires new standards and timely re-calibration of the method. These constraints provide numerous challenges for the NDA methods, particularly in the area of measurement uncertainty. This paper will discuss the challenges of these situations, mainly from a measurement and statistical point of view and provide some possible solutions to the problems encountered. Specific examples will be discussed for the segmented gamma scanner (SGS), neutron multiplicity counter (NMC) and passive neutron coincidence counter (PNCC), which are some of the most common NDA instruments at SRS

Treatment and storage of radioactive wastes at Institute for Energy Technology, Kjeller, Norway and a short survey of non-radioactive hazardous wastes in Norway

International Nuclear Information System (INIS)

Lundby, J.E.

1988-08-01

The treatment and storage of low-level and intermediate-level radioactive wastes in Norway is described. A survey of non-radioactive hazardous wastes and planned processing methods for their treatment in Norway is given. It seems that processing methods developed for radioactive wastes to a greater extent could be adopted to hazardous wastes, and that an increased interdisciplinary waste cooperation could be a positive contribution to the solution of the hazardous waste problems

Application of a microcystin extraction method specific for enzyme inhibition assays

International Nuclear Information System (INIS)

Sevilla Miguel, E.; Simienk, H.; Calvin Tienza, V.; Razquin Casquero, P.; Peleato Sanchez, M. L.; Mata Vallespin, L.

2009-01-01

A method for the determination of intracellular and dissolved microcystins in non treated water is proposed. The results obtained with this method, based on a phosphatase inhibition assay, are compared with those for HPLC- UV. Potential interferences of the phosphatase inhibition assays like pigments or the endogenous phosphatase activity present in cyanobacteria did not have any adverse effect on assay results. Besides, the recovery of microcystins in field samples with the proposed method was found to be high than 90% in all tested samples. A number of samples from different origins and appearances were also analyzed for their microcystin content. (Author) 27 refs

High-throughput kinase assays with protein substrates using fluorescent polymer superquenching

Directory of Open Access Journals (Sweden)

Weatherford Wendy

2005-05-01

Full Text Available Abstract Background High-throughput screening is used by the pharmaceutical industry for identifying lead compounds that interact with targets of pharmacological interest. Because of the key role that aberrant regulation of protein phosphorylation plays in diseases such as cancer, diabetes and hypertension, kinases have become one of the main drug targets. With the exception of antibody-based assays, methods to screen for specific kinase activity are generally restricted to the use of small synthetic peptides as substrates. However, the use of natural protein substrates has the advantage that potential inhibitors can be detected that affect enzyme activity by binding to a site other than the catalytic site. We have previously reported a non-radioactive and non-antibody-based fluorescence quench assay for detection of phosphorylation or dephosphorylation using synthetic peptide substrates. The aim of this work is to develop an assay for detection of phosphorylation of chemically unmodified proteins based on this polymer superquenching platform. Results Using a modified QTL Lightspeed™ assay, phosphorylation of native protein was quantified by the interaction of the phosphorylated proteins with metal-ion coordinating groups co-located with fluorescent polymer deposited onto microspheres. The binding of phospho-protein inhibits a dye-labeled "tracer" peptide from associating to the phosphate-binding sites present on the fluorescent microspheres. The resulting inhibition of quench generates a "turn on" assay, in which the signal correlates with the phosphorylation of the substrate. The assay was tested on three different proteins: Myelin Basic Protein (MBP, Histone H1 and Phosphorylated heat- and acid-stable protein (PHAS-1. Phosphorylation of the proteins was detected by Protein Kinase Cα (PKCα and by the Interleukin -1 Receptor-associated Kinase 4 (IRAK4. Enzyme inhibition yielded IC50 values that were comparable to those obtained using

High-throughput kinase assays with protein substrates using fluorescent polymer superquenching.

Science.gov (United States)

Rininsland, Frauke; Stankewicz, Casey; Weatherford, Wendy; McBranch, Duncan

2005-05-31

High-throughput screening is used by the pharmaceutical industry for identifying lead compounds that interact with targets of pharmacological interest. Because of the key role that aberrant regulation of protein phosphorylation plays in diseases such as cancer, diabetes and hypertension, kinases have become one of the main drug targets. With the exception of antibody-based assays, methods to screen for specific kinase activity are generally restricted to the use of small synthetic peptides as substrates. However, the use of natural protein substrates has the advantage that potential inhibitors can be detected that affect enzyme activity by binding to a site other than the catalytic site. We have previously reported a non-radioactive and non-antibody-based fluorescence quench assay for detection of phosphorylation or dephosphorylation using synthetic peptide substrates. The aim of this work is to develop an assay for detection of phosphorylation of chemically unmodified proteins based on this polymer superquenching platform. Using a modified QTL Lightspeed assay, phosphorylation of native protein was quantified by the interaction of the phosphorylated proteins with metal-ion coordinating groups co-located with fluorescent polymer deposited onto microspheres. The binding of phospho-protein inhibits a dye-labeled "tracer" peptide from associating to the phosphate-binding sites present on the fluorescent microspheres. The resulting inhibition of quench generates a "turn on" assay, in which the signal correlates with the phosphorylation of the substrate. The assay was tested on three different proteins: Myelin Basic Protein (MBP), Histone H1 and Phosphorylated heat- and acid-stable protein (PHAS-1). Phosphorylation of the proteins was detected by Protein Kinase Calpha (PKCalpha) and by the Interleukin -1 Receptor-associated Kinase 4 (IRAK4). Enzyme inhibition yielded IC50 values that were comparable to those obtained using peptide substrates. Statistical parameters that

Development and assembly of equipment for non destructive assay system control using nuclear radiation

International Nuclear Information System (INIS)

Melo, Jose Altino Tupinamba

2006-01-01

Nondestructive Assay (NDA) is applied to machines and components quality tests. These elements would not have a good performance if they were conceived without concern about the mechanical project quality, used materials, manufacture processes and inspection and maintenance methodology. There are constant developments in high level of technology with the objective of guaranteeing the components quality and the good functioning of these machines, in the mechanics, naval, aeronautical, petrochemical and steel industry, energy and nuclear generation as well. The globalization in the industry lines is a fact, leading to an increase in the multinational projects and products. The following questions arise: how to assure the high quality of components and processes? How to optimize the test methods to assure that the materials do not have defects affecting the performance of the components? The answers to the questions above are found in the application of NDA. The complex materials analysis (inhomogeneous) using NDA requires a detailed study of the sensors response signal. In this work, a measure and control system of non destructive processes was developed, using a radioactive source with a defined energy in function of the material to be analyzed. This system involves: (a) Interface of input/output (I/O) (the Hardware) and (b) graphical Interface (Software). In the non destructive analysis, it is made the comparison of the signal proceeding from the sensor with a signal preset (Set Point) or analogical signal of reference (Base Line), which is adjusted in the I/O Interface. Analyzed the signal, the system will make the decision: (a) to reject or (b) to accept the analyzed material. The I/O Interface is implemented by electronic equipment with a MCS51. The purpose of this interface is to supply conditions to exchange information, using serial RS232, between the sensor and the microcomputer. The graphical Interface (software) is written in visual C++ language (author)

Radioactivity around naval nuclear bases

International Nuclear Information System (INIS)

1989-02-01

Between 12th July and 2nd August 1988, Greenpeace took sediment samples from around four Royal Navy bases in the United Kingdom. Faslane, where nuclear powered submarines are berthed; Devonport and Rosyth where refit work is carried out and Portsmouth where US and UK nuclear submarines often visit. Samples were also taken from the US Navy base at Holy Loch, Scotland, where nuclear powered ballistic missile submarines are based. The samples were analysed at St Bartholomew Hospital Medical School, London. Identical samples were provided to the MoD personnel at Faslane and Devonport on the date taken. The purpose of carrying out the sampling programme was to highlight the fact that publicly available statistics from Ministry of Agriculture Fisheries and Food (MAFF) are too limited in range, concentrating as they do merely on radioactive levels found between tides. The findings point to the necessity of monitoring subtidal as well as intertidal areas since levels of radioactivity found in the samples at Faslane and Holy Loch were four to nine times the figures published by MAFF. Until such time as nuclear power is no longer used at sea, it is the contention of Greenpeace that a more independent and comprehensive picture of the nature of radioactive contamination from around UK naval establishments must be obtained, than that presently available from MAFF. (author)

The distribution of radioiodine administrated to pregnant mice and the effect of non radioactive iodide

International Nuclear Information System (INIS)

Okui, Toyo; Kobayashi, Satoshi

1987-01-01

Radioiodine, 131 I, which has a high fission yield in the nuclear reactor, is easily taken into the human body, accumilating in the thyroid gland, when released to the environment. 131 I was administrated orally to pregnant mice, and its transportation to the tissues, particularly the fetus, was examined closely. And further, the non-radioactive iodide, i.e., KI, was administrated to see its radiation protection effect. The transportation of 131 I to the fetus is the second highest, following the thyroid gland in the mother mouse. This transportation to the fetus becomes the higher, the larger the gestation period at which the 131 I administration is made. The administration of the non-radioactive iodide has large radiation protection effect in the thyroid gland of the mother mouse and of the fetus. But, depending on its concentration, the non-radioactive iodide may conversely increase overall exposure of the fetus. (Mori, K.)

Natural occurring radioactivity materials (NORM) in Ecuadorian oil fields

International Nuclear Information System (INIS)

Vasquez, R.; Enriquez, F.; Reinoso, T.

2008-01-01

Full text: Many natural elements contain radioactive isotopes, and most of them are present in the soil. In the gas and oil industries the most important radio nuclides are Ra-226 from the decay series of U-238, and in lower grade Ra-228 from the decay series of Th-232. Water exit from the perforation and perforation mud in the Oil towers drowns the NORM materials. Changes in temperature and pressure, allows the presence of sulphates and carbonates in pipes and internal areas of equipment. A Ra and Ba similarity leads to the selective co-precipitation in mud and incrustations of radioisotopes. A measure made in the pipe lines show that these industries generate important doses overcoming the levels of exemption and even the limits of established doses. The research was done by finding a pipe at Shushufindi 52 B well of production near by Coca city in the Ecuadorian jungle. The 'Comision Ecuatoriana de Energia Atomica' (CEEA), supervises the pipe line and accessories that are used in PETROPRODUCION fields accomplishing the radiological characterization, identifying the useless pipes and separate them in order of take care the good ones. Meanwhile the identification of the radioactive isotopes the CEEA proceed with the isolation of the radioactive disposals. From 57.830 pipes and accessories there were 1.607 useless ones, 56.223 didn't show radioactivity. Those pipes were monitored from the PETRODUCCION'S warehouses in Coca, Lago Agrio and Guarumo from September 12 th 2005 to September 12 th 2006. The CEEA is interested in NORMS because inadvertent workers may get high levels of radioactivity exposition. The Oil industries should have a manual about the complete handling of these materials. (author)

Estimation of radioactive contamination of soils from the "Balapan" and the "Experimental field" technical areas of the Semipalatinsk nuclear test site.

Science.gov (United States)

Evseeva, T; Belykh, E; Geras'kin, S; Majstrenko, T

2012-07-01

In spite of the long history of the research, radioactive contamination of the Semipalatinsk nuclear test site (SNTS) in the Republic of Kazakhstan has not been adequately characterized. Our cartographic investigation has demonstrated highly variable radioactive contamination of the SNTS. The Cs-137, Sr-90, Eu-152, Eu-154, Co-60, and Am-241 activity concentrations in soil samples from the "Balapan" site were 42.6-17646, 96-18250, 1.05-11222, 0.6-4865, 0.23-4893, and 1.2-1037 Bq kg(-1), correspondingly. Cs-137 and Sr-90 activity concentrations in soil samples from the "Experimental field" site were varied from 87 up to 400 and from 94 up to 1000 Bq kg(-1), respectively. Activity concentrations of Co-60, Eu-152, and Eu-154 were lower than the minimum detectable activity of the method used. Concentrations of naturally occurring radionuclides (K-40, Ra-226, U-238, and Th-232) in the majority of soil samples from the "Balapan" and the "Experimental field" sites did not exceed typical for surrounding of the SNTS areas levels. Estimation of risks associated with radioactive contamination based on the IAEA clearance levels for a number of key radionuclides in solid materials shows that soils sampled from the "Balapan" and the "Experimental field" sites might be considered as radioactive wastes. Decrease in specific activity of soil from the sites studied up to safety levels due to Co-60, Cs-137, Sr-90, Eu-152, Eu-154 radioactive decay and Am-241 accumulation-decay will occur not earlier than 100 years. In contrast, soils from the "Experimental field" and the "Balapan" sites (except 0.5-2.5 km distance from the "Chagan" explosion point) cannot be regarded as the radioactive wastes according safety norms valid in Russia and Kazakhstan. Copyright © 2012 Elsevier Ltd. All rights reserved.

Results after ten years of field testing low-level radioactive waste forms using lysimeters

International Nuclear Information System (INIS)

McConnell, J.W. Jr.; Rogers, R.D.; Jastrow, J.D.; Sanford, W.E.; Larsen, I.L.; Sullivan, T.M.

1995-01-01

The Field Lysimeter Investigations: Low-Level Waste Data Base Development Program is obtaining information on the performance of radioactive waste forms. Ion-exchange resins from a commercial nuclear power station were solidified into waste forms using portland cement and vinyl esterstyrene. These waste forms are being tested to: (a) obtain information on performance of waste forms in typical disposal environments, (b) compare field results with bench leach studies, (c) develop a low-level waste data base for use in performance assessment source term calculations, and (d) apply the DUST computer code to compare predicted cumulative release to actual field data. The program, funded by the Nuclear Regulatory Commission (NRC), includes observed radionuclide releases from waste forms in field lysimeters. The purpose of this paper is to present the experimental results of two lysimeter arrays over 10 years of operation, and to compare those results to bench test results and to DUST code predicted releases. Further analysis of soil cores taken to define the observed upward migration of radionuclides in one lysimeter is also presented

GPU-based parallel computing in real-time modeling of atmospheric transport and diffusion of radioactive material

Energy Technology Data Exchange (ETDEWEB)

Santos, Marcelo C. dos; Pereira, Claudio M.N.A.; Schirru, Roberto; Pinheiro, André, E-mail: jovitamarcelo@gmail.com, E-mail: cmnap@ien.gov.br, E-mail: schirru@lmp.ufrj.br, E-mail: apinheiro99@gmail.com [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Coordenacao de Pos-Graduacao e Pesquisa de Engenharia (COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear

2017-07-01

Atmospheric radionuclide dispersion systems (ARDS) are essential mechanisms to predict the consequences of unexpected radioactive releases from nuclear power plants. Considering, that during an eventuality of an accident with a radioactive material release, an accurate forecast is vital to guide the evacuation plan of the possible affected areas. However, in order to predict the dispersion of the radioactive material and its impact on the environment, the model must process information about source term (radioactive materials released, activities and location), weather condition (wind, humidity and precipitation) and geographical characteristics (topography). Furthermore, ARDS is basically composed of 4 main modules: Source Term, Wind Field, Plume Dispersion and Doses Calculations. The Wind Field and Plume Dispersion modules are the ones that require a high computational performance to achieve accurate results within an acceptable time. Taking this into account, this work focuses on the development of a GPU-based parallel Plume Dispersion module, focusing on the radionuclide transport and diffusion calculations, which use a given wind field and a released source term as parameters. The program is being developed using the C ++ programming language, allied with CUDA libraries. In comparative case study between a parallel and sequential version of the slower function of the Plume Dispersion module, a speedup of 11.63 times could be observed. (author)

GPU-based parallel computing in real-time modeling of atmospheric transport and diffusion of radioactive material

International Nuclear Information System (INIS)

Santos, Marcelo C. dos; Pereira, Claudio M.N.A.; Schirru, Roberto; Pinheiro, André; Coordenacao de Pos-Graduacao e Pesquisa de Engenharia

2017-01-01

Atmospheric radionuclide dispersion systems (ARDS) are essential mechanisms to predict the consequences of unexpected radioactive releases from nuclear power plants. Considering, that during an eventuality of an accident with a radioactive material release, an accurate forecast is vital to guide the evacuation plan of the possible affected areas. However, in order to predict the dispersion of the radioactive material and its impact on the environment, the model must process information about source term (radioactive materials released, activities and location), weather condition (wind, humidity and precipitation) and geographical characteristics (topography). Furthermore, ARDS is basically composed of 4 main modules: Source Term, Wind Field, Plume Dispersion and Doses Calculations. The Wind Field and Plume Dispersion modules are the ones that require a high computational performance to achieve accurate results within an acceptable time. Taking this into account, this work focuses on the development of a GPU-based parallel Plume Dispersion module, focusing on the radionuclide transport and diffusion calculations, which use a given wind field and a released source term as parameters. The program is being developed using the C ++ programming language, allied with CUDA libraries. In comparative case study between a parallel and sequential version of the slower function of the Plume Dispersion module, a speedup of 11.63 times could be observed. (author)

Control of the surface radioactive contamination in the field of biological research

International Nuclear Information System (INIS)

Calvo, S.; Encina, A. de la; Gaspar, J.; Macias, M. T.; Sanchez, A.; Usera, F.

2012-01-01

The manipulation of unsealed sources in biomedical research involves significant risk of radioactive contamination. the aim of this study has been to analyze the radioactive contamination occurring in the field of biomedical research, assessing its magnitude, identifying the equipment that can be contaminated with higher probability and monitoring the evolution of the contaminations production taking into account the radioisotopes and the activities uses, and the radiation protection control applied. The data used for this study correspond to a very lengthy period of time and it have been collected in the radioactive facility, of the Centro Nacional de Biotecnologia (CSIC), a very large biological research centre that can be used perfectly as a reference for this area. The results obtained show a gradual and significant decrease in the incidence of the radioactive contamination. This is due to the optimization of radiation protection standards applied and the implementation or a systematic operational radiation protection program. (Author) 13 refs.

Savannah River Site Experiences in In Situ Field Measurements of Radioactive Materials

International Nuclear Information System (INIS)

Moore, F.S.

1999-01-01

This paper discusses some of the field gamma-ray measurements made at the Savannah River Site, the equipment used for the measurements, and lessons learned during in situ identification and characterization of radioactive materials

Image-based ELISA on an activated polypropylene microtest plate--a spectrophotometer-free low cost assay technique.

Science.gov (United States)

Parween, Shahila; Nahar, Pradip

2013-10-15

In this communication, we report ELISA technique on an activated polypropylene microtest plate (APPµTP) as an illustrative example of a low cost diagnostic assay. Activated test zone in APPµTP binds a capture biomolecule through covalent linkage thereby, eliminating non-specific binding often prevalent in absorption based techniques. Efficacy of APPµTP is demonstrated by detecting human immunoglobulin G (IgG), human immunoglobulin E (IgE) and Aspergillus fumigatus antibody in patient's sera. Detection is done by taking the image of the assay solution by a desktop scanner and analyzing the color of the image. Human IgE quantification by color saturation in the image-based assay shows excellent correlation with absorbance-based assay (Pearson correlation coefficient, r=0.992). Significance of the relationship is seen from its p value which is 4.087e-11. Performance of APPµTP is also checked with respect to microtiter plate and paper-based ELISA. APPµTP can quantify an analyte as precisely as in microtiter plate with insignificant non-specific binding, a necessary prerequisite for ELISA assay. In contrast, paper-ELISA shows high non-specific binding in control sera (false positive). Finally, we have carried out ELISA steps on APPµTP by ultrasound waves on a sonicator bath and the results show that even in 8 min, it can convincingly differentiate a test sample from a control sample. In short, spectrophotometer-free image-based miniaturized ELISA on APPµTP is precise, reliable, rapid, and sensitive and could be a good substitute for conventional immunoassay procedures widely used in clinical and research laboratories. Copyright © 2013 Elsevier B.V. All rights reserved.

Unusual dileptions at RHIC a field theoretic approach based on a non-equilibrium chiral phase transition

International Nuclear Information System (INIS)

Cooper, F.

1997-01-01

This paper contains viewgraphs on unusual dileptons at Brookhaven RHIC. A field theory approach is used based on a non-equilibrium chiral phase transformation utilizing the schroedinger and Heisenberg picture

Unusual dileptions at RHIC a field theoretic approach based on a non-equilibrium chiral phase transition

Energy Technology Data Exchange (ETDEWEB)

Cooper, F. [Los Alamos National Labs., NM (United States)

1997-09-22

This paper contains viewgraphs on unusual dileptons at Brookhaven RHIC. A field theory approach is used based on a non-equilibrium chiral phase transformation utilizing the schroedinger and Heisenberg picture.

Non-Destructive Testing for Control of Radioactive Waste Package

Science.gov (United States)

Plumeri, S.; Carrel, F.

2015-10-01

Characterization and control of radioactive waste packages are important issues in the management of a radioactive waste repository. Therefore, Andra performs quality control inspection on radwaste package before disposal to ensure the compliance of the radwast characteristics with Andra waste disposal specifications and to check the consistency between Andra measurements results and producer declared properties. Objectives of this quality control are: assessment and improvement of producer radwaste packages quality mastery, guarantee of the radwaste disposal safety, maintain of the public confidence. To control radiological characteristics of radwaste package, non-destructive passive methods (gamma spectrometry and neutrons counting) are commonly used. These passive methods may not be sufficient, for instance to control the mass of fissile material contained inside radwaste package. This is particularly true for large concrete hull of heterogeneous radwaste containing several actinides mixed with fission products like 137Cs. Non-destructive active methods, like measurement of photofission delayed neutrons, allow to quantify the global mass of actinides and is a promising method to quantify mass of fissile material. Andra has performed different non-destructive measurements on concrete intermediate-level short lived nuclear waste (ILW-SL) package to control its nuclear material content. These tests have allowed Andra to have a first evaluation of the performance of photofission delayed neutron measurement and to identify development needed to have a reliable method, especially for fissile material mass control in intermediate-level long lived waste package.

Radioactive aerosols. [In Russian

Energy Technology Data Exchange (ETDEWEB)

Natanson, G L

1956-01-01

Tabulations are given presenting various published data on safe atmospheric concentrations of various radioactive and non-radioactive aerosols. Methods of determination of active aerosol concentrations and dispersion as well as the technical applications of labeled aerosols are discussed. The effect of atomic explosions are analyzed considering the nominal atomic bomb based on /sup 235/U and /sup 232/Pu equivalent to 20,000 tons of TNT.

Comparative field study: impact of laboratory assay variability on the assessment of recombinant factor IX Fc fusion protein (rFIXFc) activity.

Science.gov (United States)

Sommer, Jurg M; Buyue, Yang; Bardan, Sara; Peters, Robert T; Jiang, Haiyan; Kamphaus, George D; Gray, Elaine; Pierce, Glenn F

2014-11-01

Due to variability in the one-stage clotting assay, the performance of new factor IX (FIX) products should be assessed in this assay. The objective of this field study was to evaluate the accuracy of measuring recombinant FIX Fc fusion protein (rFIXFc) activity in clinical haemostasis laboratories using the one-stage clotting assay. Human haemophilic donor plasma was spiked with rFIXFc or BeneFIX® at 0.80, 0.20, or 0.05 IU/ml based on label potency. Laboratories tested blinded samples using their routine one-stage assay and in-house FIX plasma standard. The mean spike recoveries for BeneFIX (n=30 laboratories) were 121 %, 144 %, and 168 % of expected at nominal 0.80, 0.20, and 0.05 IU/ml concentrations, respectively. Corresponding rFIXFc spike recoveries were 88 %, 107 %, and 132 % of expected, respectively. All BeneFIX concentrations were consistently overestimated by most laboratories. rFIXFc activity was reagent-dependent; ellagic acid and silica gave higher values than kaolin, which underestimated rFIXFc. BeneFIX demonstrated significantly reduced chromogenic assay activity relative to one-stage assay results and nominal activity, while rFIXFc activity was close to nominal activity at three concentrations with better dilution linearity than the typical one-stage assay. In conclusion, laboratory- and reagent-specific assay variabilities were revealed, with progressively higher variability at lower FIX concentrations. Non-parallelism against the FIX plasma standard was observed in all one-stage assays with rFIXFc and BeneFIX, leading to significant overestimation of FIX activity at lower levels and generally high inter-laboratory variability. Compared to the accuracy currently achieved in clinical laboratories when measuring other rFIX products, most laboratories measured rFIXFc activity with acceptable accuracy and reliability using routine one-stage assay methods and commercially available plasma standards.

A prototype of radioactive waste drum monitor by non-destructive assays using gamma spectrometry

International Nuclear Information System (INIS)

Thanh, Tran Thien; Trang, Hoang Thi Kieu; Chuong, Huynh Dinh; Nguyen, Vo Hoang; Tran, Le Bao; Tam, Hoang Duc; Tao, Chau Van

2016-01-01

In this work, segmented gamma scanning and the gamma emission tomography were used to locate unknown sources in a radioactive waste drum. The simulated detector response function and full energy peak efficiency are compared to corresponding experimental data and show about 5.3% difference for an energy ranging from 81 keV to 1332.5 keV for point sources. Computation of the corresponding activity is in good agreement with the true values. - Highlights: • Segmented gamma scanning and gamma emission tomography are used to locate point source in waste drums. • The PENELOPE software is used to compute the detection efficiency of the localized point source in the waste drum. • The activity of "1"3"7Cs and "6"0Co point source could be determined with an accuracy better than 10% for air and sand matrices.

MS transport assays for γ-aminobutyric acid transporters--an efficient alternative for radiometric assays.

Science.gov (United States)

Schmitt, Sebastian; Höfner, Georg; Wanner, Klaus T

2014-08-05

Transport assays for neurotransmitters based on radiolabeled substrates are widely spread and often indispensable in basic research and the drug development process, although the use of radioisotopes is inherently coupled to issues concerning radioactive waste and safety precautions. To overcome these disadvantages, we developed mass spectrometry (MS)-based transport assays for γ-aminobutyric acid (GABA), which is the major inhibitory neurotransmitter in the central nervous system (CNS). These "MS Transport Assays" provide all capabilities of [(3)H]GABA transport assays and therefore represent the first substitute for the latter. The performance of our approach is demonstrated for GAT1, the most important GABA transporter (GAT) subtype. As GABA is endogenously present in COS-7 cells employed as hGAT1 expression system, ((2)H6)GABA was used as a substrate to differentiate transported from endogenous GABA. To record transported ((2)H6)GABA, a highly sensitive, short, robust, and reliable HILIC-ESI-MS/MS quantification method using ((2)H2)GABA as an internal standard was developed and validated according to the Center for Drug Evaluation and Research (CDER) guidelines. Based on this LC-MS quantification, a setup to characterize hGAT1 mediated ((2)H6)GABA transport in a 96-well format was established, that enables automated processing and avoids any sample preparation. The K(m) value for ((2)H6)GABA determined for hGAT1 is in excellent agreement with results obtained from [(3)H]GABA uptake assays. In addition, the established assay format enables efficient determination of the inhibitory potency of GAT1 inhibitors, is capable of identifying those inhibitors transported as substrates, and furthermore allows characterization of efflux. The approach described here combines the strengths of LC-MS/MS with the high efficiency of transport assays based on radiolabeled substrates and is applicable to all GABA transporter subtypes.

Low-level radioactive wastes

International Nuclear Information System (INIS)

Garbay, H.; Chapuis, A.M.

1988-01-01

During dismantling operations of nuclear facilities radioctive and non radioactive wastes are produced. The distinction between both kinds of wastes is not easy. In each dismantling operation special care and rules are defined for the separation of wastes. Each case must be separately studied. The volume and the surface activites are analyzed. Part of the wastes had been disposed in a public environment. The regulations, the international recommendations, thetheoretical and experimental investigations in this field are presented. A regulation principle and examples of radioactivity limits, on the basis of international recommendations, are provided. Those limits are calculated from individual radiation dose that may reach human beings [fr

Assay strategies and methods for phospholipases

International Nuclear Information System (INIS)

Reynolds, L.J.; Washburn, W.N.; Deems, R.A.; Dennis, E.A.

1991-01-01

Of the general considerations discussed, the two issues which are most important in choosing an assay are (1) what sensitivity is required to assay a particular enzyme and (2) whether the assay must be continuous. One can narrow the options further by considering substrate availability, enzyme specificity, assay convenience, or the presence of incompatible side reactions. In addition, the specific preference of a particular phospholipase for polar head group, micellar versus vesicular substrates, and anionic versus nonionic detergents may further restrict the options. Of the many assays described in this chapter, several have limited applicability or serious drawbacks and are not commonly employed. The most commonly used phospholipase assays are the radioactive TLC assay and the pH-stat assay. The TLC assay is probably the most accurate, sensitive assay available. These aspects often outweigh the disadvantages of being discontinuous, tedious, and expensive. The radioactive E. coli assay has become popular recently as an alternative to the TLC assay for the purification of the mammalian nonpancreatic phospholipases. The assay is less time consuming and less expensive than the TLC assay, but it is not appropriate when careful kinetics are required. Where less sensitivity is needed, or when a continuous assay is necessary, the pH-stat assay is often employed. With purified enzymes, when free thiol groups are not present, a spectrophotometric thiol assay can be used. This assay is ∼ as sensitive as the pH-stat assay but is more convenient and more reproducible, although the substrate is not available commercially. Despite the many assay choices available, the search continues for a convenient, generally applicable assay that is both sensitive and continuous

The microculture tetrazolium assay (MTA): another colorimetric method of testing Plasmodium falciparum chemosensitivity.

Science.gov (United States)

Delhaes, L; Lazaro, J E; Gay, F; Thellier, M; Danis, M

1999-01-01

Malarial lactate dehydrogenase (LDH), which uses 3-acetyl pyridine adenine dinucleotide as coenzyme in a reaction leading to the formation of pyruvate from L-lactate, may be used to study the susceptibility of Plasmodium falciparum to a drug in vitro. Several methods to determine the activity of this enzyme are available. One, the colorimetric method of Makler and colleagues, was modified slightly, by using sodium-2,3-bis-[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5 - carboxanilide (XTT) and following the reaction by measuring the optical density at 450 nm. Using two, culture-adapted strains of P. falciparum, this LDH assay was compared with the unmodified Makler's assay and with the isotopic microtest based on the incorporation of tritium-labelled hypoxanthine. Fresh, clinical P. falciparum isolates were also tested in the presence of several drugs, including chloroquine, mefloquine, quinine, halofantrine, atovaquone and qinghaosu derivatives. The results of the three assays were correlated for all the drugs tested except atovaquone. The two enzymatic assays are non-radioactive, rapid, reliable, inexpensive to perform and semi-automatic. However, they do require an initial parasitaemia of 2% with a haematocrit of 1.8%.

A non-isotopic assay uses bromouridine and RNA synthesis to detect DNA damage responses.

Science.gov (United States)

Hasegawa, Mayu; Iwai, Shigenori; Kuraoka, Isao

2010-06-17

Individuals with inherited xeroderma pigmentosum (XP) disorder and Cockayne syndrome (CS) are deficient in nucleotide excision repair and experience hypersensitivity to sunlight. Although there are several diagnostic assays for these disorders, the recovery of RNA synthesis (RRS) assay that can discriminate between XP cells and CS cells is very laborious. Here, we report on a novel non-radioisotope RRS assay that uses bromouridine (a uridine analog) as an alternative to (3)H-uridine. This assay can easily detect RNA polymerase I transcription in nucleoli and RNA polymerase II transcription in nuclei. The non-RI RSS assay also can rapidly detect normal RRS activity in HeLa cells. Thus, this assay is useful as a novel and easy technique for CS diagnosis. Because RRS is thought to be related to transcription-coupled DNA repair, which is triggered by the blockage of transcriptional machinery by DNA lesions, this assay may be of use for analysis of DNA repair, transcription, and/or genetic toxicity. Copyright 2010 Elsevier B.V. All rights reserved.

Public relations work in the field of disposal of radioactive wastes in the Federal Republic of Germany

International Nuclear Information System (INIS)

Schmitt, R.

1996-01-01

Many institutions have participated in public relations work concerning the final disposal of radioactive wastes in Germany since preliminary research work in this field was started in the early 1960's. The large number of institutions involved necessitates a uniform phraseology, kept up to date by permanent exchanges of information. The Press and Public Information Section of the Bundesamt fur Strahlenschutz (BfS) is responsible for co-ordination, with the aim of presenting both the general disposal concept of the Federal Government, and the work done within the scope of individual projects in the field of final waste disposal. Public relations activities are supported by the Deutsche Gesellschaft zum Bau and Betrieb von Endlagern fur Abfallstoffe mbH (DBE) which conducts public relations work at site level. Since the early sixties radioactive waste disposal policy in Germany has been based on the decision that all kinds of radioactive wastes should be disposed of in deep geological formations. One operating repository and two different disposal projects are currently under was in Germany. The paper discusses these three sites and their public relations efforts. A lot of work has been done in the public relations field in Germany, for more than 15 years now. Open, comprehensive information and reporting are essential for transparent and credible representations of the activities necessary for the disposal of radioactive waste, as well as to create a climate of confidence in order to fashion a position identity for such large-scale projects. In addition to personal contacts, target-group oriented information meetings and scientifically understandable information material are important means to achieved this end. The permanent presence of contact persons at the sites supports this objective, especially with regard to information directed towards the general public. (author)

Non-destructive assay of radioactive waste

International Nuclear Information System (INIS)

Eid, C.; Bernard, P.

1990-01-01

The nuclear fuel cycle generates a large variety of waste containing Pu. After treatment and conditioning the final destination of this waste is either to be disposed by shallow land burial or in underground geological repositories. The method of disposal is determined by the quantity of Pu contained in the waste to be disposed of. For this reason and taking into account the rigorous requirements of the safety authorities concerning the protection of people and the environment, it is most important to determine accurately the Pu contents in the waste. Separate abstracts were prepared for 28 papers in this book

Development of Spherical Near Field Model for Geological Radioactive Waste Repository

International Nuclear Information System (INIS)

Kim, S. Y.; Lee, K. J.; Chang, S. H.; Lee, K. J.; Chang, S. H.

2012-01-01

Modeling for geological radioactive waste repository can be divided into 3 parts. They are near field modeling related to engineered barrier, far field modeling related to natural barrier and biosphere modeling. In order to make the general application for safety assessment of geological waste repository, spherical geometry near field model has been developed. This model can be used quite extensively when users calculate equivalent spherical geometry for specific engineered barrier like equivalent waste radius, equivalent barrier radius and etc. Only diffusion was considered for general purpose but advection part can be updated. Goldsim and Goldsim Radionuclide Transport (RT) module were chosen and used as developing tool for the flexible modeling. Developer can freely make their own model with developer friendly graphic interface by using Goldsim. Furthermore, model with user friendly graphic interface can be developed by using Goldsim Dashboard Authoring module. The model has been validated by comparing the result with that of another model, inserting similar inputs and conditions. The model has been proved to be reasonably operating from the comparison result by validation process. Cylindrical model can be developed as a further work based on the knowledge and experience from this research

Development of Spherical Near Field Model for Geological Radioactive Waste Repository

Energy Technology Data Exchange (ETDEWEB)

Kim, S. Y.; Lee, K. J.; Chang, S. H. [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of); Lee, K. J.; Chang, S. H. [Khalifa Univ. of Science/Technology and Research, Abu Dhabi (United Arab Emirates)

2012-03-15

Modeling for geological radioactive waste repository can be divided into 3 parts. They are near field modeling related to engineered barrier, far field modeling related to natural barrier and biosphere modeling. In order to make the general application for safety assessment of geological waste repository, spherical geometry near field model has been developed. This model can be used quite extensively when users calculate equivalent spherical geometry for specific engineered barrier like equivalent waste radius, equivalent barrier radius and etc. Only diffusion was considered for general purpose but advection part can be updated. Goldsim and Goldsim Radionuclide Transport (RT) module were chosen and used as developing tool for the flexible modeling. Developer can freely make their own model with developer friendly graphic interface by using Goldsim. Furthermore, model with user friendly graphic interface can be developed by using Goldsim Dashboard Authoring module. The model has been validated by comparing the result with that of another model, inserting similar inputs and conditions. The model has been proved to be reasonably operating from the comparison result by validation process. Cylindrical model can be developed as a further work based on the knowledge and experience from this research.

Operational experience in the non-destructive assay of fissile material in General Electric's nuclear fuel fabrication facility

International Nuclear Information System (INIS)

Stewart, J.P.

1976-01-01

Operational experience in the non-destructive assay of fissile material in a variety of forms and containers and incorporation of the assay devices into the accountability measurement system for General Electric's Wilmington Fuel Fabrication Facility measurement control programme is detailed. Description of the purpose and related operational requirements of each non-destructive assay system is also included. In addition, the accountability data acquisition and processing system is described in relation to its interaction with the various non-destructive assay devices and scales used for accountability purposes within the facility. (author)

Implementation and Use of State-of-the-Art, Cell-Based In Vitro Assays.

Science.gov (United States)

Langer, Gernot

2016-01-01

assay results in a systematic fashion and a timely manner. This microplate-based assay development strategy should result in the setting up of more robust and reliable test systems that ensure and increase the confidence in the statistical significance of the actual data generated. And, although assay miniaturisation is essential in order to achieve this, most, if not all, cell-based assays can be easily reformatted and adapted to be used in this format in a straightforward manner. This synopsis aims at summarising valuable, general observations made when implementing a diverse set of functional cellular in vitro assays at Bayer Pharma AG without claiming to deeply review all of the literature available in each and every detail. In addition, phenotypic assays (Moffat et al. 2014) or label-free detection methods (Minor 2008) are not discussed. Although this essay tries to cover the most relevant technological developments in the field, it nevertheless may express personal preferences and peculiarities of the author's approach to state-of-the-art cell-based assay development. For additional reviews covering the actual field, see Wunder et al. (2008) and Michelini et al. (2010).

Lessons to be learned from radioactive waste disposal practices for non-radioactive hazardous waste management

International Nuclear Information System (INIS)

Merz, E.R.

1991-01-01

The criteria to be set up for any kind of hazardous waste disposal must always be put in perspective: 1. what are the waste characteristics? 2. what time period for safe isolation is of interest? 3. which geological disposal alternatives exist? Different approaches may be used in the short- and long-term perspective. In either case, a general procedure is recommended which involves concentrating, containing and isolating the source of toxicity, both radioactive and chemotoxic substances, as far as practicable. Waste characterization of either chemotoxic or radioactive wastes should be performed applying comparable scientifically based principles. The important question which arises is whether their hazard potential can be quantified on the basis of dose comparison regarding the morbidity effects of radiation and of chemical pollutants. Good control over the consequences of hazardous waste disposal requires threat detailed criteria for tolerable contamination of radioactive as well as chemical pollutants should be established, and that compliance with these criteria can be demonstrated. As yet, there are no well developed principles for assessing the detriment from most types of genotoxic waste other than radioactive material. The time horizon discussed for both categories of waste for their proof of safe isolation differs by a factor of about one hundred. (au)

Portable scintillation gamma-spectrometer for field measurement of radioactivity in extensive objects

International Nuclear Information System (INIS)

Besshejko, O.A.; Vishnevskij, I.N.; Denisenko, R.V.; Malyuk, I.A.; Petrosyan, Eh.E.; Karpenko, S.A.; Prijmak, V.N.

2011-01-01

The portable scintillated gamma-spectrometer for field measurement of radioactivity in the volume objects was designed. The crystal CdWO 4 that has weak dependence of light yield from temperature in combination with PMT was applied as the gamma-rays detector. The design of the device provides the possibility for measuring radioactivity of the extensive objects in 4π-geometry without background measurements. In this case the value of the efficiency needed for specific activity calculation in close approximation depends only from crystal geometry and density of measuring object and may be set as the device parameter without efficiency calibration procedure during the operation. The spectrometer does not have an auxiliary radio-active source and connecting cables in the composition. The smartphone operated under Windows Mobile is used as the control module. Bluetooth connection provides data exchange between smartphone and measurement head.

Radiorespirometic assay device

International Nuclear Information System (INIS)

Levin, G.V.; Straat, P.A.

1981-01-01

A radiorespirometic assay device is described in which the presence of microorganisms in a sample is determined by placing the sample in contact with a metabolisable radioactive labelled substrate, collecting any gas evolved, exposing a photosensitive material to the gas and determining if a spot is produced on the material. A spot indicates the presence of radioactivity showing that the substrate has been metabolized by a microorganism. Bacteria may be detected in body fluids, hospital operating rooms, water, food, cosmetics and drugs. (U.K.)

Applying thermal neutron radiography to non-destructive assays of dynamic systems

International Nuclear Information System (INIS)

Silvani, Maria I.; Almeida, Gevaldo L. de; Goncalves, Marcelo J.; Lopes, Ricardo T.

2008-01-01

Dynamic processes or systems frequently can not have their behavior directly analyzed due to safety reasons or because they require destructive assays, which can not be always afforded when high-cost equipment, devices and components are involved. Under these circumstances, some kind of non-destructive technique should be applied to preserve the safety of the personnel performing the assay, as well as the integrity of the piece being inspected. Thermal neutrons are specially suited as a tool for this purpose, thanks to their capability to pass through metallic materials, which could be utterly opaque to X-rays. This paper describes the accomplishments achieved at the Instituto de Engenharia Nuclear / CNEN, Brazil, aiming at the development of an Image Acquisition System capable to perform non-destructive assays using thermal neutrons. It is comprised of a thermal neutron source provided by the Argonauta research reactor, a converter-scintillating screen, and a CCD-based video camera optically coupled to the screen through a dark chamber equipped with a mirror. The developed system has been used to acquire 2D neutron radiographic images of static devices to reveal their inner structure, as well as movies of running systems and working devices to verify its functioning and soundness. Radiographic images of objects taken at different angles would be later on used as projections to retrieve - through a proper unfolding software - their 3D images expressed as attenuation coefficients for thermal neutrons. A quantitative performance of the system has been assessed through its Modulation Transfer Function - MTF. In order to determine this curve, unique collimators designed to simulate different spatial frequencies have been manufactured. Besides that, images of some objects have been acquired with the system being developed as well as using the conventional radiographic film, allowing thus a qualitative comparison between them. (author)

Treating radioactive effluent

International Nuclear Information System (INIS)

Kirkham, I.A.

1981-01-01

In the treatment of radioactive effluent it is known to produce a floc being a suspension of precipitates carrying radioactive species in a mother liquor containing dissolved non-radioactive salts. It is also known and accepted practice to encapsulate the floc in a solid matrix by treatment with bitumen, cement and the like. In the present invention the floc is washed with water prior to encapsulation in the solid matrix whereby to displace the mother liquor containing the dissolved non-radioactive salts. This serves to reduce the final amount of solidified radioactive waste with consequent advantages in the storage and disposal thereof. (author)

Study by X-ray diffraction of the crystalline structure versus time of a radioactive implanted coral and of a non radioactive implanted coral

International Nuclear Information System (INIS)

Irigaray, J.L.; Oudadesse, H.; Sauvage, T.; El Fadl, H.

1993-01-01

The corals used as biomaterials in bone surgery consist of 98% calcium carbonate in the form of aragonite and have orthorhombic crystalline structure. This structure changes progressively into a bone structure in an hexagonal form when the coral is implanted in cortical or spongy surroundings. For this experiment, a radioactive and a non radioactive coral have been implanted in the metaphysics of the ovine femur. The transformation of the orthorhombic structure into the hexagonal bone structure has been studied for the two types of implant. This makes it possible to verify if radioactivity modifies the process of transformation of the implanted biocoral. (K.A.) 3 refs.; 7 figs

Study by X-ray diffraction of the crystalline structure versus time of a radioactive implanted coral and of a non radioactive implanted coral

Energy Technology Data Exchange (ETDEWEB)

Irigaray, J.L.; Oudadesse, H.; Sauvage, T.; El Fadl, H. [Clermont-Ferrand-2 Univ., 63 - Aubiere (France). Lab. de Physique Corpusculaire; Lefevre, J.; Barlet, J.P. [Institut National de Recherches Agronomiques, 63 -Saint-Genes-Champanelle (France)

1993-12-31

The corals used as biomaterials in bone surgery consist of 98% calcium carbonate in the form of aragonite and have orthorhombic crystalline structure. This structure changes progressively into a bone structure in an hexagonal form when the coral is implanted in cortical or spongy surroundings. For this experiment, a radioactive and a non radioactive coral have been implanted in the metaphysics of the ovine femur. The transformation of the orthorhombic structure into the hexagonal bone structure has been studied for the two types of implant. This makes it possible to verify if radioactivity modifies the process of transformation of the implanted biocoral. (K.A.) 3 refs.; 7 figs.

Application of digital radiography for the non-destructive characterization of radioactive waste packages

International Nuclear Information System (INIS)

Lierse, C.; Goebel, H.; Kaciniel, E.; Buecherl, T.; Krebs, K.

1995-01-01

Digital radiography (DR) using gamma-rays is a powerful tool for the non-destructive determination of various parameters which are relevant within the quality control procedure of radioactive waste packages prior to an interim storage or a final disposal. DR provides information about the waste form and the extent of filling in a typical container. It can identify internal structures and defects, gives their geometric dimensions and helps to detect non-declared inner containers, shielding materials etc. From a digital radiographic image the waste matrix homogeneity may be determined and mean attenuation coefficients as well as density values for selected regions of interest can be calculated. This data provides the basis for an appropriate attenuation correction of gamma emission measurements (gamma scanning) and makes a reliable quantification of gamma emitters in waste containers possible. Information from DR measurements are also used for the selection of interesting height positions of the object which are subsequently inspected in more detail by other non-destructive methods, e. g. by transmission computerized tomography (TCT). The present paper gives important technical specifications of an integrated tomography system (ITS) which is used to perform digital radiography as well as transmission/emission computerized tomography (TCT/ECT) on radioactive waste packages. It describes the DR mode and some of its main applications and shows typical examples of radiographs of real radioactive waste drums

Transfer of radioactive and chemical pollutants into irrigated rice fields

International Nuclear Information System (INIS)

Myttenaere, C.; Mousny, J.-M.; Dabin, P.; Bittel, R.

1975-01-01

In a general study on the consequences of radioactive and chemical releases in continental waters, flooded rice fields must be considered as a very important ecosystem due to the very large quantities of water used. In order to approach as much as possible to the natural conditions (irrigated rice fields of Northern Italy) ''mini-rice fields'' were built and local practices were respected. The behavior of activation and fission products ( 137 Cs, 60 Co, 65 Zn, 51 Cr...) and heavy metals pollutants (Cd, Cr, Sn) was studied and the transfer from water to soil and plant was followed. Concentration factors were calculated for the different organs of the plant and the impact of rice ingested to the dose delivered to man was evaluated [fr

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Directory of Open Access Journals (Sweden)

Zhang PF

2015-09-01

Full Text Available Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Faculty of Science, Tanta University, Tanta, Egypt*These authors contributed equally to this workAbstract: Convenient and rapid immunofiltration assays (IFAs enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP. CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.Keywords: C-reactive proteins, point-of-care test, Glutathione capped QDs, PEGylation

The Tradescantia micronucleus assay is a highly sensitive tool for the detection of low levels of radioactivity in environmental samples.

Science.gov (United States)

Mišík, Miroslav; Krupitza, Georg; Mišíková, Katarina; Mičieta, Karol; Nersesyan, Armen; Kundi, Michael; Knasmueller, Siegfried

2016-12-01

Environmental contamination with radioactive materials of geogenic and anthropogenic origin is a global problem. A variety of mutagenicity test procedures has been developed which enable the detection of DNA damage caused by ionizing radiation which plays a key role in the adverse effects caused by radioisotopes. In the present study, we investigated the usefulness of the Tradescantia micronucleus test (the most widely used plant based genotoxicity bioassay) for the detection of genetic damage caused by environmental samples and a human artifact (ceramic plate) which contained radioactive elements. We compared the results obtained with different exposure protocols and found that direct exposure of the inflorescences is more sensitive and that the number of micronuclei can be further increased under "wet" conditions. The lowest dose rate which caused a significant effect was 1.2 μGy/h (10 h). Comparisons with the results obtained with other systems (i.e. with mitotic cells of higher plants, molluscs, insects, fish and human lymphocytes) show that the Tradescantia MN assay is one to three orders of magnitude more sensitive as other models, which are currently available. Taken together, our findings indicate that this method is due to its high sensitivity a unique tool, which can be used for environmental biomonitoring in radiation polluted areas. Copyright © 2016 Elsevier Ltd. All rights reserved.

AlphaScreen-based homogeneous assay using a pair of 25-residue artificial proteins for high-throughput analysis of non-native IgG.

Science.gov (United States)

Senga, Yukako; Imamura, Hiroshi; Miyafusa, Takamitsu; Watanabe, Hideki; Honda, Shinya

2017-09-29

Therapeutic IgG becomes unstable under various stresses in the manufacturing process. The resulting non-native IgG molecules tend to associate with each other and form aggregates. Because such aggregates not only decrease the pharmacological effect but also become a potential risk factor for immunogenicity, rapid analysis of aggregation is required for quality control of therapeutic IgG. In this study, we developed a homogeneous assay using AlphaScreen and AF.2A1. AF.2A1 is a 25-residue artificial protein that binds specifically to non-native IgG generated under chemical and physical stresses. This assay is performed in a short period of time. Our results show that AF.2A1-AlphaScreen may be used to evaluate the various types of IgG, as AF.2A1 recognizes the non-native structure in the constant region (Fc region) of IgG. The assay was effective for detection of non-native IgG, with particle size up to ca. 500 nm, generated under acid, heat, and stirring conditions. In addition, this technique is suitable for analyzing non-native IgG in CHO cell culture supernatant and mixed with large amounts of native IgG. These results indicate the potential of AF.2A1-AlphaScreen to be used as a high-throughput evaluation method for process monitoring as well as quality testing in the manufacturing of therapeutic IgG.

The non-agricultural areas of Canada and radioactivity

International Nuclear Information System (INIS)

Meyerhof, Dorothy; Marshall, Heather

1990-01-01

Approximately 90% of the Canadian land mass is non-agricultural. It is a source of food to native peoples and sport hunters. Although agricultural areas have been extensively monitored for the transfer of radionuclides through the food chain, very little work has been done on radionuclides in the natural environment in Canada. The exceptions are specific problems such as radiocesium in the lichen-caribou food chain in the Arctic and natural radioactivity in the vicinity of uranium mines. A systematic study of natural food chains is being initiated. This paper presents the results of the study so far and proposed future directions. (author)

Sensitive cell-based assay for determination of human immunodeficiency virus type 1 coreceptor tropism.

Science.gov (United States)

Weber, Jan; Vazquez, Ana C; Winner, Dane; Gibson, Richard M; Rhea, Ariel M; Rose, Justine D; Wylie, Doug; Henry, Kenneth; Wright, Alison; King, Kevin; Archer, John; Poveda, Eva; Soriano, Vicente; Robertson, David L; Olivo, Paul D; Arts, Eric J; Quiñones-Mateu, Miguel E

2013-05-01

CCR5 antagonists are a powerful new class of antiretroviral drugs that require a companion assay to evaluate the presence of CXCR4-tropic (non-R5) viruses prior to use in human immunodeficiency virus (HIV)-infected individuals. In this study, we have developed, characterized, verified, and prevalidated a novel phenotypic test to determine HIV-1 coreceptor tropism (VERITROP) based on a sensitive cell-to-cell fusion assay. A proprietary vector was constructed containing a near-full-length HIV-1 genome with the yeast uracil biosynthesis (URA3) gene replacing the HIV-1 env coding sequence. Patient-derived HIV-1 PCR products were introduced by homologous recombination using an innovative yeast-based cloning strategy. The env-expressing vectors were then used in a cell-to-cell fusion assay to determine the presence of R5 and/or non-R5 HIV-1 variants within the viral population. Results were compared with (i) the original version of Trofile (Monogram Biosciences, San Francisco, CA), (ii) population sequencing, and (iii) 454 pyrosequencing, with the genotypic data analyzed using several bioinformatics tools, i.e., the 11/24/25 rule, Geno2Pheno (2% to 5.75%, 3.5%, or 10% false-positive rate [FPR]), and webPSSM. VERITROP consistently detected minority non-R5 variants from clinical specimens, with an analytical sensitivity of 0.3%, with viral loads of ≥1,000 copies/ml, and from B and non-B subtypes. In a pilot study, a 73.7% (56/76) concordance was observed with the original Trofile assay, with 19 of the 20 discordant results corresponding to non-R5 variants detected using VERITROP and not by the original Trofile assay. The degree of concordance of VERITROP and Trofile with population and deep sequencing results depended on the algorithm used to determine HIV-1 coreceptor tropism. Overall, VERITROP showed better concordance with deep sequencing/Geno2Pheno at a 0.3% detection threshold (67%), whereas Trofile matched better with population sequencing (79%). However, 454

Portable scintillation gamma-spectrometer for field measurement of radioactivity in extensive objects

Directory of Open Access Journals (Sweden)

O. A. Bezshyyko

2011-12-01

Full Text Available The portable scintillated gamma-spectrometer for field measurement of radioactivity in the volume objects was designed. The crystal CdWO4 that has weak dependence of light yield from temperature in combination with PMT was applied as the gamma-rays detector. The design of the device provides the possibility for measuring radioactivity of the extensive objects in 4-geometry without background measurements. In this case the value of the efficiency needed for specific activity calculation in close approximation depends only from crystal geometry and density of measuring object and may be set as the device parameter without efficiency calibration procedure during the operation. The spectrometer does not have an auxiliary radio-active source and connecting cables in the composition. The smartphone operated under Windows Mobile is used as the control module. Bluetooth connection provides data exchange between smartphone and measurement head.

Results after nine years of field testing low-level radioactive waste forms using lysimeters

International Nuclear Information System (INIS)

McConnell, J.W. Jr.; Rogers, R.D.; Jastrow, J.D.; Sanford, W.E.; Sullivan, T.M.

1995-01-01

The Field Lysimeter Investigations: Low-Level Waste Data Base Development Program is obtaining information on the performance of radioactive waste forms. Ion-exchange resins from a nuclear power station were solidified into waste forms using Portland cement and vinyl ester-styrene. These waste forms are being tested to develop a low-level waste data base and to obtain information on survivability of waste forms in a disposal environment. This paper reviews radionuclide releases from those waste forms in the first 9 years of sampling. Included is a discussion of the recently discovered upward migration of radionuclides. Also, lysimeter data are applied to a performance assessment source term model, and initial results are presented

Sensitive non-radioactive determination of aminotransferase stereospecificity for C-4' hydrogen transfer on the coenzyme.

Science.gov (United States)

Jomrit, Juntratip; Summpunn, Pijug; Meevootisom, Vithaya; Wiyakrutta, Suthep

2011-02-25

A sensitive non-radioactive method for determination of the stereospecificity of the C-4' hydrogen transfer on the coenzymes (pyridoxal phosphate, PLP; and pyridoxamine phosphate, PMP) of aminotransferases has been developed. Aminotransferase of unknown stereospecificity in its PLP form was incubated in (2)H(2)O with a substrate amino acid resulted in PMP labeled with deuterium at C-4' in the pro-S or pro-R configuration according to the stereospecificity of the aminotransferase tested. The [4'-(2)H]PMP was isolated from the enzyme protein and divided into two portions. The first portion was incubated in aqueous buffer with apo-aspartate aminotransferase (a reference si-face specific enzyme), and the other was incubated with apo-branched-chain amino acid aminotransferase (a reference re-face specific enzyme) in the presence of a substrate 2-oxo acid. The (2)H at C-4' is retained with the PLP if the aminotransferase in question transfers C-4' hydrogen on the opposite face of the coenzyme compared with the reference aminotransferase, but the (2)H is removed if the test and reference aminotransferases catalyze hydrogen transfer on the same face. PLP formed in the final reactions was analyzed by LC-MS/MS for the presence or absence of (2)H. The method was highly sensitive that for the aminotransferase with ca. 50 kDa subunit molecular weight, only 2mg of the enzyme was sufficient for the whole test. With this method, the use of radioactive substances could be avoided without compromising the sensitivity of the assay. Copyright © 2011 Elsevier Inc. All rights reserved.

Radiation and environmental radioactivity

International Nuclear Information System (INIS)

Muhamat Omar; Ismail Sulaiman; Zalina Laili

2015-01-01

This book is written based on 25 years authors experience especially in scientifc research of radiation and environmental radioactivity field at Malaysian Nuclear Agency (Nuklear Malaysia). Interestingly, from the authors experience in managing the services and consultancies for radiological environmental monitoring, it is also helpful in preparing the ideas for this book. Although this book focuses on Malaysian radiation information environmental radioactivity, but the data collected by the international bodies are also included in this book.

Method for assay of radioactivity in waste soil

International Nuclear Information System (INIS)

Bramlitt, E.T.; Willhoite, S.B.

1991-01-01

Contaminated soil is a result of many nuclear operations. During facility decommissioning or site cleanup, it may be packaged for disposal. The waste soil must be assayed for contaminants to follow transport regulations and waste handling facility requirements. Methods used for assay include the following: (1) sampling the ground before excavation and assuming ground data apply to soil when packaged; (2) analyzing samples taken from the soil added to a package; (3) counting radiation at the exterior of the package; and (4) measuring neutron absorption by packaged waste soil. The Defense Nuclear Agency (DNA) worked with Eberline Instruments Corporation (EIC) to develop an automated assay method for the waste stream in a plutonium-contaminated soil cleanup at Johnston Atoll in the North Pacific Ocean. The perfected method uses a personal computer, an electronic weighing scale, and a programmable radiation counter. Computer programs get weight and radiation counts at frequent intervals as packages fill, calculate activity in the waste, and produce reports. The automated assay method is an efficient one-person routine that steadfastly collects data and produces a comprehensive record on packaged waste

Assessment of statistic analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with α-hexylcinnamic aldehyde as an example

International Nuclear Information System (INIS)

Takeyoshi, Masahiro; Sawaki, Masakuni; Yamasaki, Kanji; Kimber, Ian

2003-01-01

The murine local lymph node assay (LLNA) is used for the identification of chemicals that have the potential to cause skin sensitization. However, it requires specific facility and handling procedures to accommodate a radioisotopic (RI) endpoint. We have developed non-radioisotopic (non-RI) endpoint of LLNA based on BrdU incorporation to avoid a use of RI. Although this alternative method appears viable in principle, it is somewhat less sensitive than the standard assay. In this study, we report investigations to determine the use of statistical analysis to improve the sensitivity of a non-RI LLNA procedure with α-hexylcinnamic aldehyde (HCA) in two separate experiments. Consequently, the alternative non-RI method required HCA concentrations of greater than 25% to elicit a positive response based on the criterion for classification as a skin sensitizer in the standard LLNA. Nevertheless, dose responses to HCA in the alternative method were consistent in both experiments and we examined whether the use of an endpoint based upon the statistical significance of induced changes in LNC turnover, rather than an SI of 3 or greater, might provide for additional sensitivity. The results reported here demonstrate that with HCA at least significant responses were, in each of two experiments, recorded following exposure of mice to 25% of HCA. These data suggest that this approach may be more satisfactory--at least when BrdU incorporation is measured. However, this modification of the LLNA is rather less sensitive than the standard method if employing statistical endpoint. Taken together the data reported here suggest that a modified LLNA in which BrdU is used in place of radioisotope incorporation shows some promise, but that in its present form, even with the use of a statistical endpoint, lacks some of the sensitivity of the standard method. The challenge is to develop strategies for further refinement of this approach

Assessment of statistic analysis in non-radioisotopic local lymph node assay (non-RI-LLNA) with alpha-hexylcinnamic aldehyde as an example.

Science.gov (United States)

Takeyoshi, Masahiro; Sawaki, Masakuni; Yamasaki, Kanji; Kimber, Ian

2003-09-30

The murine local lymph node assay (LLNA) is used for the identification of chemicals that have the potential to cause skin sensitization. However, it requires specific facility and handling procedures to accommodate a radioisotopic (RI) endpoint. We have developed non-radioisotopic (non-RI) endpoint of LLNA based on BrdU incorporation to avoid a use of RI. Although this alternative method appears viable in principle, it is somewhat less sensitive than the standard assay. In this study, we report investigations to determine the use of statistical analysis to improve the sensitivity of a non-RI LLNA procedure with alpha-hexylcinnamic aldehyde (HCA) in two separate experiments. Consequently, the alternative non-RI method required HCA concentrations of greater than 25% to elicit a positive response based on the criterion for classification as a skin sensitizer in the standard LLNA. Nevertheless, dose responses to HCA in the alternative method were consistent in both experiments and we examined whether the use of an endpoint based upon the statistical significance of induced changes in LNC turnover, rather than an SI of 3 or greater, might provide for additional sensitivity. The results reported here demonstrate that with HCA at least significant responses were, in each of two experiments, recorded following exposure of mice to 25% of HCA. These data suggest that this approach may be more satisfactory-at least when BrdU incorporation is measured. However, this modification of the LLNA is rather less sensitive than the standard method if employing statistical endpoint. Taken together the data reported here suggest that a modified LLNA in which BrdU is used in place of radioisotope incorporation shows some promise, but that in its present form, even with the use of a statistical endpoint, lacks some of the sensitivity of the standard method. The challenge is to develop strategies for further refinement of this approach.

Analysis of cesium-137 vertical distribution in the profile of plowed chernozems at different schemes of their assaying

International Nuclear Information System (INIS)

Paramonova, T.A.; Komissarova, O.L.; Belyaev, V.R.; Ivanov, M.M.

2016-01-01

In 2011-2015 the assessment of profile cesium-137 distribution in agrocenosis of chernozem zone on the territory of the Plavsk radioactive spot in Tula region formed after the Chernobyl accident has been carried out. It is shown that up until now non-uniformity of cesium-137 vertical distribution over the plowed chernozems profile may be occurred, it should be taken into account at radioecological survey of post-Chernobyl landscapes. For correct evaluation of radioecological state of plowed soils their systematic monitoring on the base of preliminary analysis of cesium-137 distribution and also with the account of agrotechnical peculiarities of various crops cultivation is recommended. On the Plavsk radioactive spot territory the most adequate assessments of cesium-137 stores in plowed chernozems one can obtain on the base of assaying the upper 30-cm soil depth, including not only current topsoil, but also old-arable horizon formed by deep rehabilitation plowing [ru

Radiometric measurements on the fabrication of non-destructive assay standards for WIPP-Performance Demonstration Program

International Nuclear Information System (INIS)

Wong, A.S.; Marshall, R.S.

1997-04-01

The Inorganic Elemental Analysis Group of LANL has prepared several different sets of working reference materials (WRMs). These WRMs are prepared by blending quantities of nuclear materials (plutonium, americium, and enriched uranium) with diatomaceous earth. The blends are encapsulated in stainless steel cylinders. These WRMs are being measured as blind controls in neutron and gamma based non-destructive assay (NDA) instruments. Radiometric measurements on the blending homogeneity and verification on a set of sixty three plutonium based WRMs are discussed in this paper

Development of plastic scintillator based food radioactivity contamination monitoring system

International Nuclear Information System (INIS)

Parihar, A.; Sahani, R.M.; Mahala, V.K.; Vaijapurkar, S.G.

2016-01-01

Radioactivity is naturally present in soil, water and food stuffs. Food can be contaminated after discharge of radioactivity into the environment from industries that concentrate natural radionuclide and from civil or military nuclear operations. The contamination can be in three ways; by direct deposition, through the food chain and induced radioactivity due to exposure of high neutron flux. The health effects on human depend on the type of radionuclide and the length of time people are exposed to it. The studies of fission product behaviour in the food chain have revealed radionuclide Strontium-90, Caesium 137 and Iodine-131 are of major concern. Plastic scintillator is already developed indigenously at Defence Laboratory, Jodhpur. Efforts has been made to develop a portable field instrument using plastic scintillator for assessment of beta ( 90 Sr) and gamma ( 137 Cs and 131 I) radioactivity in food

A National system for the Management of Non-nuclear Radioactive Waste in Sweden

International Nuclear Information System (INIS)

Lindhe, J. C.

2004-01-01

The Swedish government in May 2002 set up a non-standing committee for non-nuclear radioactive waste. The objective was to suggest a national system for the management of all types of non-nuclear radioactive waste with special consideration to the principle of polluter pays and the responsibility of the producers. The committee delivered its recommendations to the government at the end of last year. Funding for future costs for nuclear waste management and final storage is collected in a state governed funding system. For non-nuclear waste, however, there are no means today to secure the funding. If a company goes bankrupt and leaves radioactive waste behind it might be up to the taxpayers to pay for its safe management. This is due to the fact that the cost for the waste is paid at the time one wants to dispose of it and it is usually the last owner of a product etc. that has to pay. Sometimes the price comes as a surprise and the owner might not have the money available. Thus the waste might be kept longer than otherwise and might even end up as orphan waste. To solve this dilemma the committee recommends a funding system in parallel with the system for the nuclear waste. The cost for the waste should be paid up front before the waste has been created. E.g. when a customer buys a product the cost for the future waste management would be included in the price and he will not have to pay for this the day he disposes the product by returning it to the producer or leaves it to a waste-collecting organisation. It should be the responsibility of the producer (manufacturer, importer or re-seller) to guarantee the funding for the waste management. In summary the non-nuclear radioactive waste is divided into three main groups: waste from products, waste from practices and other waste. Waste from products includes household products as well as products used in research, industry and hospitals etc. For this category it is easy to identify a producer who imports or

Medical investigation on the effects of radioactivity in the field of petrochemistry

International Nuclear Information System (INIS)

Li Xinluan; Gao Min; Shao Hua; Liu Wei; Li Qingjie; Hou Dianjun; Qiao Jianwei; Lin Haiqun; Shang Ximei

2005-01-01

Objective: To investigate the health condition of the people who work in the petrochemical field and to provide the basis for the policy-making of control and survey of the radiology disease. Methods: To measure the volume of radioactivity individual in the environment of the people who work in the field of petrochemistry and consequently to cheek and analysize the changes in physical and biochemistry. Results: The annual volume of radioactivity exposure individual is 5.667 mSv·a -1 , >50 mSv·a -1 20. Consequently, this volume can induce many abnormalities in human body. Compare to the control group, the abnormalities include high blood pressure of abnormal EKG, eye disease, low white blood ceels, the changed rate of CD 4 /CD 8 , distortion of DNA and appearances of higher micronucleus. Conclusion: Long time exposure to low volume of radiation will cause some of the abnormalities to humanbody. Therefore, the health protection measures should be strengthened. (authors)

Regulatory inspection practices for radioactive and non-radioactive waste management facilities

International Nuclear Information System (INIS)

Roy, Amitava

2017-01-01

Management of nuclear waste plays an important role in the nuclear energy programme of the country. India has adopted the Closed Fuel Cycle option, where the spent nuclear fuel is treated as a material of resource and the nuclear waste is wealth. Closed fuel cycle aims at recovery and recycle of valuable nuclear materials in to reactors as fuel and also separation of useful radio isotopes for the use in health care, agriculture and industry. India has taken a lead role in the waste management activities and has reached a level of maturity over a period of more than forty decades. The nuclear waste management primarily comprises of waste characterization, segregation, conditioning, treatment, immobilization of radionuclides in stable and solid matrices and interim retrievable storage of conditioned solid waste under surveillance. The waste generated in a nuclear facility is in the form of liquid and solid, and it's classification depends on the content of radioactivity. The liquid waste is characterized as Low level (LLW), Intermediate level (ILW) and High Level (HLW). The LLW is relatively large in volume and much lesser radioactive. The LLW is subjected to chemical precipitation using various chemicals based on the radionuclides present, followed by filtration, settling, ion exchange and cement fixation. The conditioning and treatment processes of ILW uses ion exchange, alkali hydrolysis for spent solvent, phase separation and immobilization in cement matrix. The High Level Waste (HLW), generated during spent fuel reprocessing and containing more than 99 percent of the total radioactivity is first subjected to volume reduction/concentration by evaporation and then vitrified in a meIter using borosilicate glass. Presently, Joule Heated Ceramic Meter is used in India for Vitrification process. Vitrified waste products (VWP) are stored for interim period in a multibarrier, air cooled facility under surveillance

Evaluation of Two Lyophilized Molecular Assays to Rapidly Detect Foot-and-Mouth Disease Virus Directly from Clinical Samples in Field Settings.

Science.gov (United States)

Howson, E L A; Armson, B; Madi, M; Kasanga, C J; Kandusi, S; Sallu, R; Chepkwony, E; Siddle, A; Martin, P; Wood, J; Mioulet, V; King, D P; Lembo, T; Cleaveland, S; Fowler, V L

2017-06-01

Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot-and-mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories. However, transport of samples to these centralized facilities can be a lengthy process that can impose delays on critical decision making. These concerns have motivated work to evaluate simple-to-use technologies, including molecular-based diagnostic platforms, that can be deployed closer to suspect cases of FMD. In this context, FMD virus (FMDV)-specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) and real-time RT-PCR (rRT-PCR) assays, compatible with simple sample preparation methods and in situ visualization, have been developed which share equivalent analytical sensitivity with laboratory-based rRT-PCR. However, the lack of robust 'ready-to-use kits' that utilize stabilized reagents limits the deployment of these tests into field settings. To address this gap, this study describes the performance of lyophilized rRT-PCR and RT-LAMP assays to detect FMDV. Both of these assays are compatible with the use of fluorescence to monitor amplification in real-time, and for the RT-LAMP assays end point detection could also be achieved using molecular lateral flow devices. Lyophilization of reagents did not adversely affect the performance of the assays. Importantly, when these assays were deployed into challenging laboratory and field settings within East Africa they proved to be reliable in their ability to detect FMDV in a range of clinical samples from acutely infected as well as convalescent cattle. These data support the use of highly sensitive molecular assays into field settings for simple and rapid detection of FMDV. © 2015 The Authors. Transboundary and Emerging Diseases Published by Blackwell Verlag GmbH.

Non-fuel cycle radioactive waste policy in Turkey

International Nuclear Information System (INIS)

Izmir, A.I.; Uslu, I.

2001-01-01

2000. By categorizing the disposal of 'solid', 'liquid' and 'gaseous' waste, an efficient management system is achieved. Solid radioactive waste consists mainly of protective clothing, plastic sheets and bags, gloves, masks, organs and tissues, animal carcasses, filters, overshoes, paper wipes, towels, metal and glass, hand tools, discarded radiopharmaceuticals containers and discarded equipment. It generally contains a relatively low level of radioactivity when compared to liquid wastes. Special consideration should always be given to the management of contaminated sharp objects, such as needles and syringes, scalpel blades, blood lancets, glass ampoules, etc. Short-lived solid radioactive wastes are stored in the waste storage rooms of the facilities until their activities reduce to an acceptable level to be released to the municipal waste disposal area. The liquid waste can be discharged to sewage system when its activity concentration come down to permissible discharge level which is based on IAEA S S-70. The liquid waste from iodine therapy patients is mostly collected and stored in storage tanks. If the treated patient number is low the waste should be collected separately in shielded drums and stored in waste storage rooms of the facilities until its activity concentration level decreases to an acceptable level. b) Management of Sealed Sources. Sealed radiation sources are widely used in industry, medicine and research in Turkey. Sealed sources have a life cycle, which begins with manufacture and culminates in disposal. Each source life cycle comprises a number of potential stages. A source life cycle can involve individuals in the following key organisations: regulator, manufacturer, Original Equipment Manufacturer, distributor, user (one or subsequent users), waste management organisation, and operator of storage or disposal facility. The large number of organisations potentially involved and their interactions mean that life cycles tend to be complex and can

Disposal of radioactive and other hazardous wastes

International Nuclear Information System (INIS)

Boge, R.; Bergman, C.; Bergvall, S.; Gyllander, C.

1989-01-01

The purpose of the workshop was discuss legal, scientific and practical aspects of disposal of low- and intermediate-level radioactive waste and other types of hazardous waste. During the workshop the non-radioactive wastes discussed were mainly wastes from energy production, but also industrial, chemical and household wastes. The workshop gave the participants the opportunity to exchange information on policies, national strategies and other important matters. A number of invited papers were presented and the participants brought background papers, describing the national situation, that were used in the working groups. One of the main aims of the workshop was to discuss if the same basic philosophy as that used in radiation protection could be used in the assessment of disposal of non-radioactive waste, as well as to come up with identifications of areas for future work and to propose fields for research and international cooperation. The main text of the report consists of a summary of the discussions and the conclusions reached by the workshop

Non-invasive terahertz field imaging inside parallel plate waveguides

DEFF Research Database (Denmark)

Iwaszczuk, Krzysztof; Andryieuski, Andrei; Lavrinenko, Andrei

2011-01-01

We present a non-invasive broadband air photonic method of imaging of the electric field of THz pulses propagating inside a tapered parallel plate waveguide. The method is based on field-enhanced second harmonic generation of the fundamental laser beam in an external electric field. We apply...

Determination of the effective diffusion coefficient of water through cement-based materials when applying an electrical field

International Nuclear Information System (INIS)

Wattez, T.

2013-01-01

The safety and the reliability of a radioactive waste repository rely essentially on the confinement ability of the waste package and the storing structure. In the case of the low-level and intermediate level short-lived radioactive waste, the confinement property, relying on solid matrices made of cement-based materials, is assessed through a natural diffusion test, using a radioactive tracer, from which an effective diffusion coefficient is deduced. The evolution of the materials and more particularly the enhancement of the confinement properties of cement-based materials lead to test duration from a couple of months to a couple of years. The main objective of the present work involves the determination of the effective diffusion coefficient of reference chemical species, in our case the tritiated water, within a shorter time. The theoretical foundation is based on the description of ionic species mass transfer under the effects of an electrical field. With the definitions of a precise experimental protocol and of a formation factor, considered as an intrinsic topological feature of the porous network, it is possible to determine the effective diffusion coefficient of tritiated water for various types of concretes and mortars, and this within a few hours only. The comparison between the developed accelerated test, based on the application of a constant electrical field, and the normed natural diffusion test, using tritiated water, underlined two critical issues. First, omitting the impact of the radioactive decay of tritium during a natural diffusion test, leads to a non-negligible underestimation of the effective diffusion coefficient. Second, maintaining samples in high relative humidity conditions after casting is essential in order to avoid contrasted and unrelated results when performing the electrokinetic tests. Eventually, the validation of the electrokinetics technique, main objective of this work, rests on the assessment of the theoretical hypothesis

Development of field-based real-time reverse transcription-polymerase chain reaction assays for detection of Chikungunya and O'nyong-nyong viruses in mosquitoes.

Science.gov (United States)

Smith, Darci R; Lee, John S; Jahrling, Jordan; Kulesh, David A; Turell, Michael J; Groebner, Jennifer L; O'Guinn, Monica L

2009-10-01

Chikungunya (CHIK) and O'nyong-nyong (ONN) are important emerging arthropod-borne diseases. Molecular diagnosis of these two viruses in mosquitoes has not been evaluated, and the effects of extraneous mosquito tissue on assay performance have not been tested. Additionally, no real-time reverse transcription-polymerase chain reaction (RT-PCR) assay exists for detecting ONN virus (ONNV) RNA. We describe the development of sensitive and specific real-time RT-PCR assays for detecting CHIK and ONN viral RNA in mosquitoes, which have application for field use. In addition, we compared three methods for primer/probe design for assay development by evaluating their sensitivity and specificity. This comparison resulted in development of virus-specific assays that could detect less than one plaque-forming unit equivalent of each of the viruses in mosquitoes. The use of these assays will aid in arthropod-borne disease surveillance and in the control of the associated diseases.

Environmental pathways of radioactivity to man

International Nuclear Information System (INIS)

Johns, T.F.

1979-12-01

The report reviews and discusses the environmental pathways by which radioactive materials can lead to the irradiation of man, in a way that should be understood by non-specialists who have neither the time nor the knowledge to study all of the relevant literature on this subject. The role of these environmental pathways in the general structure of radiological protection is considered, and the various mechanisms which lead to the dispersion or re-concentration of radioactive materials are discussed at some length. Particular groups of radionuclides from the nuclear power industry are considered in some detail. Similarly the question of the corresponding pathways from naturally-occurring radioactive materials is covered. The doses to animals and plants resulting from the nuclear industry are examined, and it is concluded that there is no reason to expect that these doses will lead to significant harm. Finally a summary is presented, and it is noted that it has been possible to obtain a very extensive knowledge of the behaviour of radionuclides in the environment only because of the extreme sensitivity of the techniques available for their detection, identification and assay. As a result a fund of knowledge has been built up about the behaviour of radioactive materials in the environment which is far more extensive than our knowledge of the behaviour of many highly toxic chemicals which are also discharged into the environment. (UK)

Radioactive waste data base through the net: A tool to improve the development of waste management

International Nuclear Information System (INIS)

Sanhueza Mir, Azucena

2003-01-01

One of the duties in Chilean Commission for Nuclear Energy (CCHEN) is the timely reply to the International Atomic Energy Agency (IAEA) Net enable waste management data base (NEWMDB) in the waste management field. This duty is carried out by the Radioactive Waste Management Section. CCHEN has complete this data base from about one decade ago. Through the time, the data base has changed according to new available information technologies, to the point that the access using the international net is a need today. The NEWMDB objective is to exchange information and knowledge between member states related to radioactive waste management situation and to conform a world inventory of radioactive waste. The Chilean experience got from the NEWMDB first data collection cycle (1999-2000) is presented here, and recommendations to be considered for incorporation in the domestic waste management system are exposed. In so doing, the data base answer should be easy to do and totally understood by everyone whose job is waste management around the world, in the context of the glossary, criteria and conventions on this data base is supported. The composition of the NEWMDB considers a General Frame which indicates the way in which the waste management is enfaced in the country, regulations, authorities, policies, infrastructure; a Waste Classification matrix which give the equivalence between proper country waste classification and that recommended by IAEA; Waste Data which give the quantities and situation of waste in the different steps of the management such as: conditioned waste, unconditioned stored waste, etc. Finally, the Sustainable Development for radioactive waste management Indicators (SDI) for the safety and environmental radioactive waste management are estimated (Au)

Recent developments at French atomic energy commission relating to non destructive nuclear waste assay by using electron accelerator

International Nuclear Information System (INIS)

Lvoussi, A.; Romeyer-Dhebey, J.; Jallu, F.; Passard, C.; Mariani, A.; Recroix, H.; Payan, E.; Denis, C.; Loridon, J.; Buisson, A.; Nurdin, G.; Allano, J.; Jaureguy, J.C.

2000-01-01

An important program is currently in progress at several laboratories over the world for the development of sensitive, practical non-destructive assay techniques for the quantification of low level transuranics (TRU) in solid wastes. The wide variety of materials and contaminants, the low concentrations and large volumes involve, all make this kind of assay a complicated affair. Over the last few years, considerable progress has been made in the field of assay techniques for low level contaminated wastes. This report describes the methods being developed at French Atomic Energy Commission (C.E.A.) in Cadarache to assay high density TRU waste packages by using photon, neutron or both photon and neutron as interrogating particles. All of these particles are produced by using a pulsed electron linear accelerator from which the photons are produced following Bremsstrahlung phenomena on a heavy metallic converter and the neutrons are generated in appropriate low level photoneutron threshold target (e.g. Beryllium). The dynamic of photonuclear interactions and photoneutron production, use of an electron linear accelerator as a particle source, experimental and electronics details, experimental results, simulation to experiment performances and future experimental and theoretical studies are discussed. (authors)

National facilities for the management of institutional radioactive waste in Romania: 25 years of operation for radioactive waste treatment plant, Bucharest-Magurele, 15 years of operation for national radioactive repository, Baita-Bihor

International Nuclear Information System (INIS)

Rotarescu, Gh.; Turcanu, C.; Dragolici, F.; Lungu, L.; Nicu, M.; Cazan, L.; Matei, G.; Guran, V.

1999-01-01

The management of the non-fuel cycle radioactive wastes in Romania is centralized at IFIN-HH in the Radioactive Waste Treatment Plant (STDR) Bucharest-Magurele and the National Repository of Radioactive Waste (DNDR) Baita-Bihor. From November 1974 to November 1999 there were treated at STDR nearly 26,000 m 3 LLAW, 2,100 m 3 LLSW and 4,000 spent sources resulting over 5,500 conditioned packages disposed at DNDR. After 25 years of operation for STDR and 15 years of operation for DNDR an updating programme started in 1991. The R and D programme will improve the basic knowledge and waste management practices for the increasing of nuclear safety in the field. (authors)

A flow cytometric assay technology based on quantum dots-encoded beads

International Nuclear Information System (INIS)

Wang Haiqiao; Liu Tiancai; Cao Yuancheng; Huang Zhenli; Wang Jianhao; Li Xiuqing; Zhao Yuandi

2006-01-01

A flow cytometric detecting technology based on quantum dots (QDs)-encoded beads has been described. Using this technology, several QDs-encoded beads with different code were identified effectively, and the target molecule (DNA sequence) in solution was also detected accurately by coupling to its complementary sequence probed on QDs-encoded beads through DNA hybridization assay. The resolution of this technology for encoded beads is resulted from two longer wavelength fluorescence identification signals (yellow and red fluorescent signals of QDs), and the third shorter wavelength fluorescence signal (green reporting signal of fluorescein isothiocyanate (FITC)) for the determination of reaction between probe and target. In experiment, because of QDs' unique optical character, only one excitation light source was needed to excite the QDs and probe dye FITC synchronously comparing with other flow cytometric assay technology. The results show that this technology has present excellent repeatability and good accuracy. It will become a promising multiple assay platform in various application fields after further improvement

Low-level radioactive wastes. A bibliography of additions to the Energy Data Base in 1981

International Nuclear Information System (INIS)

Grissom, M.C.

1981-12-01

Access to information on management of low-level radioactive wastes is provided by this special bibliography and other products of the Technical Information Center. Citations to 215 references on low-level radioactive wastes added to the Energy Data Base in 1981 are presented. The abstracts are grouped in broad subject categories. Within each category the arrangement is by report number for reports, followed by non-reports in reverse chronological order. These citations are to research reports, journal articles, books, patents, theses, and conference papers. Five indexes, each preceded by a brief description, are provided: Corporate Author, Personal Author, Subject, Contract Number, and Report Number

Dynamic optical tweezers based assay for monitoring early drug resistance

International Nuclear Information System (INIS)

Wu, Xiaojing; Zhu, Siwei; Feng, Jie; Zhang, Yuquan; Min, Changjun; Yuan, X-C

2013-01-01

In this letter, a dynamic optical tweezers based assay is proposed and investigated for monitoring early drug resistance with Pemetrexed-resistant non-small cell lung cancer (NSCLC) cell lines. The validity and stability of the method are verified experimentally in terms of the physical parameters of the optical tweezers system. The results demonstrate that the proposed technique is more convenient and faster than traditional techniques when the capability of detecting small variations of the response of cells to a drug is maintained. (letter)

The E.C.C. programme in the field of radioactive wastes

International Nuclear Information System (INIS)

Orlowski, S.

1976-01-01

After a description of the aims of the E.C.C. in the field of radioactive wastes, the contents and the structure of the programme developed for this purpose as well as its time schedule and financing, are presented. The state of work progress and the first results of the actions taken, whose application is marked by an active cooperation of numerous national experts are quoted [fr

Separation of non-stationary multi-source sound field based on the interpolated time-domain equivalent source method

Science.gov (United States)

Bi, Chuan-Xing; Geng, Lin; Zhang, Xiao-Zheng

2016-05-01

In the sound field with multiple non-stationary sources, the measured pressure is the sum of the pressures generated by all sources, and thus cannot be used directly for studying the vibration and sound radiation characteristics of every source alone. This paper proposes a separation model based on the interpolated time-domain equivalent source method (ITDESM) to separate the pressure field belonging to every source from the non-stationary multi-source sound field. In the proposed method, ITDESM is first extended to establish the relationship between the mixed time-dependent pressure and all the equivalent sources distributed on every source with known location and geometry information, and all the equivalent source strengths at each time step are solved by an iterative solving process; then, the corresponding equivalent source strengths of one interested source are used to calculate the pressure field generated by that source alone. Numerical simulation of two baffled circular pistons demonstrates that the proposed method can be effective in separating the non-stationary pressure generated by every source alone in both time and space domains. An experiment with two speakers in a semi-anechoic chamber further evidences the effectiveness of the proposed method.

Magnetic nanostructures: radioactive probes and recent developments

International Nuclear Information System (INIS)

Prandolini, M J

2006-01-01

The miniaturization of magnetic sensors and storage devices down to the nano-scale leads to drastic changes in magnetic phenomena compared with the same devices with a larger size. Excited-nuclear-probe (radioactive probe) techniques are ideal for investigating these new magnetic nanostructures. By observing the magnetic hyperfine fields (and in some cases the electric-field-gradients (EFGs)) at the nuclei of radioactive probes, microscopic information about the magnetic environment of the probes is acquired. The magnetic hyperfine field is particularly sensitive to the s-spin polarization of the conduction electrons and to the orbital magnetic moment of the probe atom. Three methods of inserting radioactive probes into magnetic nanostructures are presented; neutron activation, recoil implantation and 'soft-landing', followed by descriptions of their application to selected examples. In some cases, these methods offer the simultaneous creation and observation of new magnetic materials at the atomic scale. This review focuses firstly on the induced magnetism in noble-metal spacer layers between either ferromagnetic (FM) or FM/antiferromagnetic (AFM) layers in a trilayer structure. Using the method of low-temperature nuclear orientation, the s-spin polarization of noble-metal probes was measured and was found to be very sensitive to the magnetic properties at both the FM and AFM interfaces. Secondly, the recoil implantation of radioactive Fe probes into rare-earth hosts and d-band alloys and subsequent measurement using time-differential perturbed angular distribution offer the possibility of controlling the chemical composition and number of nearest-neighbours. This method was used to prepare local 3d-magnetic clusters in a non-magnetic matrix and to observe their magnetic behaviour. Finally, non-magnetic radioactive probes were 'soft-landed' onto Ni surfaces and extremely lattice-expanded ultrathin Ni films. By measuring the magnetic hyperfine fields and EFGs at

Evaluation of NDA techniques applying to the measurement of alpha-activities in medium or low level radioactive reprocessing wastes (part 5). The development of LiF coating semiconductor detector

Energy Technology Data Exchange (ETDEWEB)

Hattori, Takatoshi; Ishii, Keiichiro [Central Research Inst. of Electric Power Industry, Komae, Tokyo (Japan). Komae Research Lab.

1988-12-01

In order to design the neutron detection system in the non-destructive assay device applying to the measurement of alpha-activity in medium or low level radioactive reprocessing wastes, we examined the property of the LiF coating semiconductor detector applying to gamma-radiation field. The sensitivity for thermal neutron of LiF coating ion implanted detector is 1.6 cps/nv, and reduced to half in about 300 R/h gamma-radiation field. Compared with the other neutron detectors, for example proportional counters, the performance of this detector is almost similar to that of them. This new detector has a merit to be possible to use a dry battery instead of a high voltage supply. (author).

Development of a non invasion real-time PCR assay for the quantitation of chicken parvovirus in fecal swabs

Science.gov (United States)

The present study describes the development of a real time Taqman polymerase chain reaction (PCR) assay using a fluorescent labeled probe for the detection and quantitation of chicken parvovirus (ChPV) in feces. The primers and probes were designed based on the nucleotide sequence of the non struct...

Enzyme-linked immunosorbent assays for Z-DNA.

OpenAIRE

Thomas, M J; Strobl, J S

1988-01-01

Dot blot and transblot enzyme-linked immunosorbent assays (e.l.i.s.a.) are described which provide sensitive non-radioactive methods for screening Z-DNA-specific antisera and for detecting Z-DNA in polydeoxyribonucleotides and supercoiled plasmids. In the alkaline phosphatase dot blot e.l.i.s.a., Z-DNA, Br-poly(dG-dC).poly(dG-dC), or B-DNA, poly(dG-dC).poly(dG-dC), poly(dA-dT).poly(dA-dT), Br-poly(dI-dC).poly(dI-dC), or salmon sperm DNA were spotted onto nitrocellulose discs and baked. The e....

Supergravity, Non-Conformal Field Theories and Brane-Worlds

CERN Document Server

Gherghetta, Tony; Gherghetta, Tony; Oz, Yaron

2002-01-01

We consider the supergravity dual descriptions of non-conformal super Yang-Mills theories realized on the world-volume of Dp-branes. We use the dual description to compute stress-energy tensor and current correlators. We apply the results to the study of dilatonic brane-worlds described by non-conformal field theories coupled to gravity. We find that brane-worlds based on D4 and D5 branes exhibit a localization of gauge and gravitational fields. We calculate the corrections to the Newton and Coulomb laws in these theories.

Beyond low-level activity: On a 'non-radioactive' gas mantle

International Nuclear Information System (INIS)

Poljanc, Karin; Steinhauser, Georg; Sterba, Johannes H.; Buchtela, Karl; Bichler, Max

2007-01-01

Gas mantles for camping gas lanterns sometimes contain thorium compounds. During the last years, the use of thorium-free gas mantles has become more and more popular due to the avoidance of a radioactive heavy metal. We investigated a gas mantle type that is declared to be 'non-radioactive' and that can be bought in Austria at the moment. Methods used were Instrumental Neutron Activation Analysis (INAA), γ-spectroscopy, and Liquid Scintillation Counting (LSC). We found massive thorium contents of up to 259 mg per gas mantle. Leaching experiments showed that only 0.4% of the Th but approximately 90% of the decay products of 232 Th can be leached under conditions simulating sucking and chewing with human saliva. In this paper, the investigation of these gas mantles including the consideration of the environmental hazard caused by disposed mantles and the health hazard for unsuspecting consumers is presented and legal consequences are discussed for this fraud

A Novel High Sensitivity Sensor for Remote Field Eddy Current Non-Destructive Testing Based on Orthogonal Magnetic Field

Directory of Open Access Journals (Sweden)

Xiaojie Xu

2014-12-01

Full Text Available Remote field eddy current is an effective non-destructive testing method for ferromagnetic tubular structures. In view of conventional sensors’ disadvantages such as low signal-to-noise ratio and poor sensitivity to axial cracks, a novel high sensitivity sensor based on orthogonal magnetic field excitation is proposed. Firstly, through a three-dimensional finite element simulation, the remote field effect under orthogonal magnetic field excitation is determined, and an appropriate configuration which can generate an orthogonal magnetic field for a tubular structure is developed. Secondly, optimized selection of key parameters such as frequency, exciting currents and shielding modes is analyzed in detail, and different types of pick-up coils, including a new self-differential mode pick-up coil, are designed and analyzed. Lastly, the proposed sensor is verified experimentally by various types of defects manufactured on a section of a ferromagnetic tube. Experimental results show that the proposed novel sensor can largely improve the sensitivity of defect detection, especially for axial crack whose depth is less than 40% wall thickness, which are very difficult to detect and identify by conventional sensors. Another noteworthy advantage of the proposed sensor is that it has almost equal sensitivity to various types of defects, when a self-differential mode pick-up coil is adopted.

Development of a novel non-radioactive cell-based method for the screening of SGLT1 and SGLT2 inhibitors using 1-NBDG.

Science.gov (United States)

Chang, Hung-Chi; Yang, Su-Fu; Huang, Ching-Chun; Lin, Tzung-Sheng; Liang, Pi-Hui; Lin, Chun-Jung; Hsu, Lih-Ching

2013-08-01

Sodium-coupled glucose co-transporters SGLT1 and SGLT2 play important roles in intestinal absorption and renal reabsorption of glucose, respectively. Blocking SGLT2 is a novel mechanism for lowering the blood glucose level by inhibiting renal glucose reabsorption and selective SGLT2 inhibitors are under development for treatment of type 2 diabetes. Furthermore, it has been reported that perturbation of SGLT1 is associated with cardiomyopathy and cancer. Therefore, both SGLT1 and SGLT2 are potential therapeutic targets. Here we report the development of a non-radioactive cell-based method for the screening of SGLT inhibitors using COS-7 cells transiently expressing human SGLT1 (hSGLT1), CHO-K1 cells stably expressing human SGLT2 (hSGLT2), and a novel fluorescent d-glucose analogue 1-NBDG as a substrate. Our data indicate that 1-NBDG can be a good replacement for the currently used isotope-labeled SGLT substrate, (14)C-AMG. The Michaelis constant of 1-NBDG transport (0.55 mM) is similar to that of d-glucose (0.51 mM) and AMG (0.40 mM) transport through hSGLT1. The IC50 values of a SGLT inhibitor phlorizin for hSGLT1 obtained using 1-NBDG and (14)C-AMG were identical (0.11 μM) in our cell-based system. The IC50 values of dapagliflozin, a well-known selective SGLT2 inhibitor, for hSGLT2 and hSGLT1 determined using 1-NBDG were 1.86 nM and 880 nM, respectively, which are comparable to the published results obtained using (14)C-AMG. Compared to (14)C-AMG, the use of 1-NBDG is cost-effective, convenient and potentially more sensitive. Taken together, a non-radioactive system using 1-NBDG has been validated as a rapid and reliable method for the screening of SGLT1 and SGLT2 inhibitors.

Non-local charges in local quantum field theory

International Nuclear Information System (INIS)

Buchholz, D.; Lopuszanski, J.T.; Rabsztyn, S.

1985-05-01

Non-local charges are studied in the general setting of local quantum field theory. It is shown, that these charges can be represented as polynomials in the incoming respectively outgoing fields with coefficients (kernels) which are subject to specific constraints. For the restricted class of models of a scalar, massive, self interacting particle in four dimensions, a more detailed analysis shows that all non-local charges of the generic type (genus 2) are products of generators of the Poincare group. This analysis, which is based on the macroscopic causality properties of the S-matrix, seems to indicate that less trivial examples of non-local charges can only exist in two dimensions. (orig.)

The fundamentals of the Russian Federation National Policy in the non-nuclear fuel cycle radioactive waste management

International Nuclear Information System (INIS)

Latypov, E.M.; Rikunov, V.A.

1995-01-01

Extensive manufacture and use of sources of ionizing radiation result inevitably in the generation of a considerable amount of radioactive waste. The crucial objective within the context of the general problem of radioactive waste management involves the safe isolation of radioactive waste from the environment for the entire period of the existence of their potential hazardous impacts upon it. The complex nature of the problem requires substantial efforts to be placed for the establishment of an integrated radioactive waste management system providing a national control in medicine, industry and science. To this end, the fundamentals of the national policy for the safe management of radioactive waste from non-nuclear fuel cycle activities are being developed in the Russian Federation. The essential components of the national policy are: development of a scientifically sound concept of radioactive waste management; adoption of legislative documents such as standards and acts, relevant to this area; implementation and enforcement of state regulations and supervision of the relevant activities; development of a national programme on radioactive waste management; provision and maintaining of a national radioactive waste inventory; radiation monitoring

A biorthogonal decomposition for the identification and simulation of non-stationary and non-Gaussian random fields

Energy Technology Data Exchange (ETDEWEB)

Zentner, I. [IMSIA, UMR EDF-ENSTA-CNRS-CEA 9219, Université Paris-Saclay, 828 Boulevard des Maréchaux, 91762 Palaiseau Cedex (France); Ferré, G., E-mail: gregoire.ferre@ponts.org [CERMICS – Ecole des Ponts ParisTech, 6 et 8 avenue Blaise Pascal, Cité Descartes, Champs sur Marne, 77455 Marne la Vallée Cedex 2 (France); Poirion, F. [Department of Structural Dynamics and Aeroelasticity, ONERA, BP 72, 29 avenue de la Division Leclerc, 92322 Chatillon Cedex (France); Benoit, M. [Institut de Recherche sur les Phénomènes Hors Equilibre (IRPHE), UMR 7342 (CNRS, Aix-Marseille Université, Ecole Centrale Marseille), 49 rue Frédéric Joliot-Curie, BP 146, 13384 Marseille Cedex 13 (France)

2016-06-01

In this paper, a new method for the identification and simulation of non-Gaussian and non-stationary stochastic fields given a database is proposed. It is based on two successive biorthogonal decompositions aiming at representing spatio–temporal stochastic fields. The proposed double expansion allows to build the model even in the case of large-size problems by separating the time, space and random parts of the field. A Gaussian kernel estimator is used to simulate the high dimensional set of random variables appearing in the decomposition. The capability of the method to reproduce the non-stationary and non-Gaussian features of random phenomena is illustrated by applications to earthquakes (seismic ground motion) and sea states (wave heights).

Radioactivity

International Nuclear Information System (INIS)

2002-01-01

This pedagogical document presents the origin, effects and uses of radioactivity: where does radioactivity comes from, effects on the body, measurement, protection against radiations, uses in the medical field, in the electric power industry, in the food (ionization, radio-mutagenesis, irradiations) and other industries (radiography, gauges, detectors, irradiations, tracers), and in research activities (dating, preservation of cultural objects). The document ends with some examples of irradiation levels (examples of natural radioactivity, distribution of the various sources of exposure in France). (J.S.)

The equipment for low radioactivity measurements in industrial and field conditions

International Nuclear Information System (INIS)

Malik, R.; Owczarczyk, A.; Szpilowski, S.; Zenczykiewicz, Z.

1992-01-01

The equipment for low radioactivity measurements in industrial and field conditions has been worked out. Three scintillation detectors applied work in coincidence system. Their scintillation crystals are divided one to another by lead shieldings. All measuring system is situated in a lead container with lead cover. The measuring vessel fills practically all free volume of the lead container. Their shape ensures the best possible measurement geometry. (author). 3 figs

Radioactive waste disposal; Le stockage des dechets radioactifs (aspects non techniques)

Energy Technology Data Exchange (ETDEWEB)

Petit, J.C

1998-04-01

A deep gap, reflecting a persisting fear, separates the viewpoints of the experts and that of the public on the issue of the disposal of nuclear WASTES. The history of this field is that of the proliferation with time of spokesmen who pretend to speak in the name of the both humans and non humans involved. Three periods can be distinguished: 1940-1970, an era of contestation and confusion when the experts alone represents the interest of all; 1970-1990, an era of contestation and confusion when spokespersons multiply themselves, generating the controversy and the slowing down of most technological projects; 1990-, an era of negotiation, when viewpoints, both technical and non technical, tend to get closer and, let us be optimistic, leading to the overcome of the crisis. We show that, despite major differences, the options and concepts developed by the different actors are base on two categories of resources, namely Nature and Society, and that the consensus is built up through their `hydridation`. we show in this part that the perception of nuclear power and, in particular of the underground disposal of nuclear wastes, involves a very deep psychological substrate. Trying to change mentalities in the domain by purely scientific and technical arguments is thus in vain. The practically instinctive fear of radioactivity, far from being due only to lack of information (and education), as often postulated by scientists and engineers, is rooted in archetypical structures. These were, without doubt, reactivated in the 40 s by the traumatizing experience of the atomic bomb. In addition, anthropological-linked considerations allow us to conclude that he underground disposal of wastes is seen as a `rape` and soiling of Mother Earth. This contributes to explaining, beyond any rationality, the refusal of this technical option by some persons. However, it would naturally be simplistic and counter-productive to limit all controversy in this domain to these psychological aspects

Radioactive source monitoring system based on RFID and GPRS

International Nuclear Information System (INIS)

He Haiyang; Zhou Hongliang; Zhang Hongjian; Zhang Sheng; Zhou Junru; Weng Guojie

2011-01-01

Nuclear radiation produced by radioactive source is harmful to the health of human body, and the lost and theft of radioactive source will cause environmental pollution and social panic. In order to solve the abnormal leaks, accidental loss, theft and other problems of the radioactive source, a radioactive source monitoring system based on RFID, GPS, GPRS and GSM technology is put forward. Radiation dose detector and GPS wireless location module are used to obtain the information of radiation dose and location respectively, RFID reader reads the status of a tag fixed on the bottom of the radioactive source. All information is transmitted to the remote monitoring center via GPRS wireless transmission. There will be an audible and visual alarm when radiation dose is out of limits or the state of radioactive source is abnormal, and the monitoring center will send alarming text messages to the managers through GSM Modem at the same time. Thus, the functions of monitoring and alarming are achieved. The system has already been put into operation and is being kept in functional order. It can provide stable statistics as well as accurate alarm, improving the supervision of radioactive source effectively. (authors)

Radioactive scrap metal (RSM) inventory ampersand tracking system and prototype RSM field survey

International Nuclear Information System (INIS)

Thomas, T.R.

1994-09-01

Based on very preliminary information, it has been estimated that the radioactive scrap metal (RSM) inventories at DOE facilities amount to about 1.5 million tons and a much larger amount will be generated from decontamination and decommissioning of surplus DOE facilities. To implement a national DOE program for beneficial reuse of RSM, it will be necessary to known the location and characteristics of RSM inventories that are available and will be generated to match them with product demands. It is the intent of this task to provide a standardized methodology via a RSM database for recording, tracking, and reporting data on RSM inventories. A multiple relational database in dBASE IV was designed and a PC-based code was written in Clipper 5.0 syntax to expedite entry, editing, querying, and reporting of RSM survey data. The PC based-code, the multiple relational database files, and other external files used by the code to generate reports and queries constitute a customized software application called the RSM Inventory ampersand Tracking System (RSM I ampersand TS). A prototype RSM field survey was conducted at the Nevada Test Site (NTS) to demonstrate the field use of the RSM I ampersand TS and logistics of conducting the survey. During the demonstration, about 50 tons of RSM were sized, characterized, sorted, and packaged in transport containers

Preparation process of a stable non radioactive carrier intended for radiodiagnosis

International Nuclear Information System (INIS)

Molinski, V.J.; Wilczewski, J.A.

1976-01-01

The description is given of a stable non radioactive carrier with a pH of around 2 and around 4, for cardiological studies, exploring the blood in circulation and exploring the brain, after labelling with sup(99m)Tc. It includes human serum albumin (SAH) and stannous tartrate, the proportion in weight between the SAH and stannous tartrate being between around 50:1 and around 150:1 [fr

Improvement of equipment needed for the detection and characterization of nuclear material and radioactive samples at borders

International Nuclear Information System (INIS)

Arlt, R.; Kovar, A.; Swoboda, M.; Beck, P.; Duftschmid, K.E.; Lewis, W.

2001-01-01

Nuclear radiation measurement instruments and devices for the detection and characterisation of nuclear material and radioactive isotopes at borders play a decisive role in the set of technical measures needed to deal with the problem of illicit trafficking. Therefore the availability of a range of devices with adequate performance at affordable prices is essential. The needed equipment can be classified into the following categories: Detection equipment (border monitors and radiation pagers); Hand held devices for neutron/gamma source search and in-field characterisation of radiation sources by customs inspectors; NDA (non-destructive assay) instruments for in-depth investigation of a seized item by experts either in the field or under laboratory conditions. Instruments and techniques, which use destructive assay methods, are not considered in this paper. The most recent overview on the performance of such instruments is given in the Final Report of ITRAP (Illicit Trafficking Radiation Detection Assessment Program). According to the findings of ITRAP, although in all above mentioned categories equipment is commercially available, deficiencies and shortcomings were identified. In this paper we describe our effort in addressing some of the most essential equipment problems, identified in ITRAP - the sensitivity of border monitors with respect to the detection of nuclear material and the performance and usability of hand held and portable devices needed for in-field characterisation of seized items by customs inspectors

Field test results for radioactive waste drum characterization with Waste Inspection Tomography (WIT)

Energy Technology Data Exchange (ETDEWEB)

Bernardi, R.T. [Bio-Imaging Research, Inc., Lincolnshire, IL (United States)

1997-11-01

This paper summarizes the design, fabrication, factory testing, evaluation and demonstration of waste inspection tomography (WIT). WIT consists of a self-sufficient, mobile semi-trailer for Non-Destructive Evaluation and Non-Destructive Assay (NDE/NDA) characterization of nuclear waste drums using X-ray and gamma-ray tomographic techniques. The 23-month WIT Phase I initial test results include 2 MeV Digital Radiography (DR), Computed Tomography (CT), Anger camera imaging, Single Photon Emission Computed Tomography (SPECT), Gamma-Ray Spectroscopy, Collimated Gamma Scanning (CGS), and Active and Passive Computed Tomography (A&PCT) using a 1.4 mCi source of {sup 166}Ho. These techniques were initially demonstrated on a 55-gallon phantom drum with three simulated waste matrices of combustibles, heterogeneous metals, and cement using check sources of gamma active isotopes. Waste matrix identification, isotopic identification, and attenuation-corrected gamma activity determination were all demonstrated nondestructively and noninvasively. Preliminary field tests results with nuclear waste drums are summarized. WIT has inspected drums with 0 to 20 grams plutonium 239. The minimum measured was 0.131 gram plutonium 239 in cement. 8 figs.

Formation of filtration fields close to near-surface radioactive waste storages

International Nuclear Information System (INIS)

Mart'yanov, V.V.

2008-01-01

Data on the formation of filtration fields in the location of near-surface radioactive waste storages for the conditions of uniformly isotropic properties of bearing strata are demonstrated. The possibility for changing parameters of mean-caused ground flow depending on water permeability of the storages and their dimensions in plan is noted. Comparison of different filtration fields permits to determine a state of its isolating properties. Assessment criteria of the storage engineering barriers integrity are given. Conditions for uniformly isotropic properties of bearing strata by three scenarios, when engineering barriers of the storage are waterproof, distracted or lost protective properties in full, have been determined. Changing filtration field, geochemical and radiochemical situations in bearing strata are noted to represent one of basic characteristics of the integrity of the storage [ru

Some important concepts in development of radioimmunoassay (RIA) for hormones, viruses and drugs. [Peptide hormones, non-peptide hormones

Energy Technology Data Exchange (ETDEWEB)

Shah, K B; Mani, R S [Bhabha Atomic Research Centre, Bombay (India). Radiopharmaceuticals Section

1981-01-01

Radioimmunoassay (RIA) procedures for the quantitative measurement of polypeptide and steroid hormones and other substances of medical and biological interest constitute one of the most important and rapidly expanding groups of applications of radioactive tracers in analytical chemistry and the life sciences. The method consists in setting up assays wherein the isotopically tagged substance is allowed to compete with its non-radioactive counterpart for the limited binding sites of a specific antibody. The degree of competitive inhibition of binding of labelled tracer is determined by measuring the radioactivity of the bound or unbound (free) complex, and comparing with the corresponding values for standard solutions of known concentration. This paper outlines the salient features, and specific problems associated with the preparation, purification of immunoreactive labelled tracers, and other suitable RIA reagents, the stability and storage conditions and standardisation of assay procedure. The characteristics of the assay systems have been investigated in detail and regular quality control has been instituted for evaluating various statistical parameters, inter-assay and intra-assay variances, and effective shelf life of the RIA reagents, with specific reference to assays of insulin growth hormones and thyroid and pregnancy hormones.

Effect of Radioactivity of Technetium-99m on the Autosterilization Process of non-sterile Tetrofosmin Kits

Directory of Open Access Journals (Sweden)

Widyastuti Widyastuti

2017-03-01

Full Text Available Technetium-99m labeled radiopharmaceutical is commonly used in nuclear medicines as a diagnostic agent, by mixing the sterile kit with Tc-99m. Manufacturing of kits requires an aseptic facility which need to be well designed and maintained according to cGMP, since mostly kits can not be terminally sterilized. Radiopharmaceuticals as pharmaceuticals containing radionuclide is assumed to have an autosterilization property, but correlation between radioactivity and capability of killing microorganisms has to be studied so far. The aim of this study is to investigate the effect of radioactivity on the autosterilization process of radiopharmaceuticals. The study was carried out by adding Tc-99m of various radioactivity into non-sterile tetrofosmin kits, then the samples were tested for sterility. Sterile tetrofosmin kit and non-sterile kit with no Tc-99m added will be used as a negative control and positive control respectively. The sterility was tested using standard direct inoculation method, by inoculating samples in culture media for both bacteria and fungi and observing qualitatively within 14 days. The results showed that the samples with radioactivity of 1, 3 and 5 mCi changed the clarity of the media to turbid, conformed with the performance of positive controls but samples with radioactivity of 10 mCi and 20 mCi did not change the clarity of the media, conformed with the performance of negative control, indicating neither growth of bacteria nor fungi. It is concluded that Tc-99m behaves as an autosterilizing agent at certain radioactivity. Therefore the preparation of Tc-99m radiopharmaceutical can be considered as terminal sterilization rather than aseptic preparation.

Performance values for non destructive assay (NDA) techniques applied to safeguards: the 2002 evaluation by the ESARDA NDA Working Group

International Nuclear Information System (INIS)

Guardini, S.

2003-01-01

The first evaluation of NDA performance values undertaken by the ESARDA Working Group for Standards and Non Destructive Assay Techniques (WGNDA) was published in 1993. Almost 10 years later the Working Group decided to review those values, to report about improvements and to issue new performance values for techniques which were not applied in the early nineties, or were at that time only emerging. Non-Destructive Assay techniques have become more and more important in recent years, and they are used to a large extent in nuclear material accountancy and control both by operators and control authorities. As a consequence, the performance evaluation for NDA techniques is of particular relevance to safeguards authorities in optimising Safeguards operations and reducing costs. Performance values are important also for NMAC regulators, to define detection levels, limits for anomalies, goal quantities and to negotiate basic audit rules. This paper presents the latest evaluation of ESARDA Performance Values (EPVs) for the most common NDA techniques currently used for the assay of nuclear materials for Safeguards purposes. The main topics covered by the document are: techniques for plutonium bearing materials: PuO 2 and MOX; techniques for U-bearing materials; techniques for U and Pu in liquid form; techniques for spent fuel assay. This issue of the performance values is the result of specific international round robin exercises, field measurements and ad hoc experiments, evaluated and discussed in the ESARDA NDA Working Group. (author)

Radioactive waste management glossary

International Nuclear Information System (INIS)

1988-01-01

The Waste Management Glossary defines over 300 terms in the English language that have special meanings when they are used in the context of radioactive waste management. The Glossary is intended to provide a consistent reference for these terms for specialists in this field. It also will assist non-specialists who read IAEA reports dealing with waste management. This is the second edition of the Glossary. It is intended to update and replace its predecessor, TECDOC-264, that was issued in 1982. (author)

Discovery of novel poly(ADP-ribose) glycohydrolase inhibitors by a quantitative assay system using dot-blot with anti-poly(ADP-ribose)

International Nuclear Information System (INIS)

Okita, Naoyuki; Ashizawa, Daisuke; Ohta, Ryo; Abe, Hideaki; Tanuma, Sei-ichi

2010-01-01

Poly(ADP-ribosyl)ation, which is mainly regulated by poly(ADP-ribose) polymerase (PARP) and poly(ADP-ribose) glycohydrolase (PARG), is a unique protein modification involved in cellular responses such as DNA repair and replication. PARG hydrolyzes glycosidic linkages of poly(ADP-ribose) synthesized by PARP and liberates ADP-ribose residues. Recent studies have suggested that inhibitors of PARG are able to be potent anti-cancer drug. In order to discover the potent and specific Inhibitors of PARG, a quantitative and high-throughput screening assay system is required. However, previous PARG assay systems are not appropriate for high-throughput screening because PARG activity is measured by radioactivities of ADP-ribose residues released from radioisotope (RI)-labeled poly(ADP-ribose). In this study, we developed a non-RI and quantitative assay system for PARG activity based on dot-blot assay using anti-poly(ADP-ribose) and nitrocellulose membrane. By our method, the maximum velocity (V max ) and the michaelis constant (k m ) of PARG reaction were 4.46 μM and 128.33 μmol/min/mg, respectively. Furthermore, the IC50 of adenosine diphosphate (hydroxymethyl) pyrrolidinediol (ADP-HPD), known as a non-competitive PARG inhibitor, was 0.66 μM. These kinetics values were similar to those obtained by traditional PARG assays. By using our assay system, we discovered two novel PARG inhibitors that have xanthene scaffold. Thus, our quantitative and convenient method is useful for a high-throughput screening of PARG specific inhibitors.

Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors.

Science.gov (United States)

Wynn, Daniel; Deo, Sapna; Daunert, Sylvia

2017-01-01

Bacterial whole cell-based biosensors have been genetically engineered to achieve selective and reliable detection of a wide range of hazardous chemicals. Although whole-cell biosensors demonstrate many advantages for field-based detection of target analytes, there are still some challenges that need to be addressed. Most notably, their often modest shelf life and need for special handling and storage make them challenging to use in situations where access to reagents, instrumentation, and expertise are limited. These problems can be circumvented by developing biosensors in Bacillus spores, which can be engineered to address all of these concerns. In its sporulated state, a whole cell-based biosensor has a remarkably long life span and is exceptionally resistant to environmental insult. When these spores are germinated for use in analytical techniques, they show no loss in performance, even after long periods of storage under harsh conditions. In this chapter, we will discuss the development and use of whole cell-based sensors, their adaptation to spore-based biosensors, their current applications, and future directions in the field. © 2017 Elsevier Inc. All rights reserved.

Calibration with MCNP of NaI detector for the determination of natural radioactivity levels in the field.

Science.gov (United States)

Cinelli, Giorgia; Tositti, Laura; Mostacci, Domiziano; Baré, Jonathan

2016-05-01

In view of assessing natural radioactivity with on-site quantitative gamma spectrometry, efficiency calibration of NaI(Tl) detectors is investigated. A calibration based on Monte Carlo simulation of detector response is proposed, to render reliable quantitative analysis practicable in field campaigns. The method is developed with reference to contact geometry, in which measurements are taken placing the NaI(Tl) probe directly against the solid source to be analyzed. The Monte Carlo code used for the simulations was MCNP. Experimental verification of the calibration goodness is obtained by comparison with appropriate standards, as reported. On-site measurements yield a quick quantitative assessment of natural radioactivity levels present ((40)K, (238)U and (232)Th). On-site gamma spectrometry can prove particularly useful insofar as it provides information on materials from which samples cannot be taken. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

The hell of a radioactivity

International Nuclear Information System (INIS)

Lambert, G.

2004-01-01

Nuclear accident, contaminations, pollutions and never-ending hazards: nuclear energy and especially radioactivity frightens the collective unconscious in an unreasoned way. The object of this book is to explain in a simple manner the bases and real values of natural radioactivity, nuclear energy and even of nuclear bombs. Without any will of proselytism, but with all the necessary scientific exactness, the author uses a novel style and the fancied story of a young couple for the exploration of the industrial and natural environment of radioactivity. From field measurements to laboratory visits, the reader, whatever his scientific culture, will be able to make his own opinion about this important question of science and society. (J.S.)

Non-fuel cycle radioactive waste policy in Turkey

International Nuclear Information System (INIS)

Demirel, H.

2003-01-01

Radioactive wastes generated in Turkey are mostly low level radioactive waste generated from the operation of one research reactor, research centers and universities, hospitals, and from radiological application of various industries. Disused sealed sources which potentially represent medium and high radiological risks in Turkey are mainly Am-241, Ra-226, Kr-85, Co-60, Ir-192 and Cs-137. All radioactive waste produced in Turkey is collected, segregated, conditioned and stored at CWPSF. Main components of the facility are listed below: Liquid waste is treated in chemical processing unit where precipitation is applied. Compactable solids are compressed in a compaction cell. Spent sources are embedded into cement mortar with their original shielding. If the source activities are in several millicuries, sometimes dismantling is applied and segregated sources are conditioned in shielded drums. Due to increasing number of radiation and nuclear related activities, the waste facility of CNAEM is now becoming insufficient to meet the storage demand of the country. TAEA is now in a position to establish a new radioactive waste management facility and studies are now being carried out on the selection of best place for the final storage of processed radioactive wastes. Research and development studies in TAEA should continue in radioactive waste management with the aim of improving data, models, and concepts related to long-term safety of disposal of long-lived waste

Adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided versus empirical chemotherapy in unresectable non-small cell lung cancer.

Science.gov (United States)

Moon, Yong Wha; Sohn, Joo Hyuk; Kim, Yong Tai; Chang, Hyun; Jeong, Jae Heon; Lee, Young Joo; Chang, Joon; Kim, Se Kyu; Jung, Minkyu; Hong, Soojung; Choi, Sung Ho; Kim, Joo-Hang

2009-10-01

We retrospectively compared adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided and empirical chemotherapies for unresectable non-small cell lung cancer (NSCLC) in this case-control study. Unresectable NSCLC patients receiving ATP-CRA-guided platinum-based doublets as first-line therapy were enrolled as cases (n=27; 14 platinum-sensitive and 13 platinum-resistant patients). Performance status, stage, and chemotherapeutic regimen-matched patients receiving empirical chemotherapy were selected from the retrospective database as controls (n=93) in a case to control ratio of approximately 1:3. Response rate and survival (progression-free; overall) in both groups were not significantly different. However, the platinum-sensitive subgroup by ATP-CRA showed a higher response rate than the empirical group (71 versus 38%; p=0.023) with a trend toward longer progression-free survival (8.7 versus 4.8 months for platinum-sensitive versus empirical; p=0.223) and overall survival (not reached versus 12.6 months for platinum-sensitive versus empirical for p=0.134). ATP-CRA may be helpful in selecting platinum-responsive patients in unresectable NSCLC. We consider that nonplatinum doublets in platinum-resistant patients by ATP-CRA may be a more adapted approach than platinum-based doublets in future clinical trials.

Radioactive air sampling methods

CERN Document Server

Maiello, Mark L

2010-01-01

Although the field of radioactive air sampling has matured and evolved over decades, it has lacked a single resource that assimilates technical and background information on its many facets. Edited by experts and with contributions from top practitioners and researchers, Radioactive Air Sampling Methods provides authoritative guidance on measuring airborne radioactivity from industrial, research, and nuclear power operations, as well as naturally occuring radioactivity in the environment. Designed for industrial hygienists, air quality experts, and heath physicists, the book delves into the applied research advancing and transforming practice with improvements to measurement equipment, human dose modeling of inhaled radioactivity, and radiation safety regulations. To present a wide picture of the field, it covers the international and national standards that guide the quality of air sampling measurements and equipment. It discusses emergency response issues, including radioactive fallout and the assets used ...

Proton radioactivity at non-collective prolate shape in high spin state of 94Ag

International Nuclear Information System (INIS)

Aggarwal, Mamta

2010-01-01

We predict proton radioactivity and structural transitions in high spin state of an excited exotic nucleus near proton drip line in a theoretical framework and investigate the nature and the consequences of the structural transitions on separation energy as a function of temperature and spin. It reveals that the rotation of the excited exotic nucleus 94 Ag at excitation energies around 6.7 MeV and angular momentum near 21h generates a rarely seen prolate non-collective shape and proton separation energy becomes negative which indicates proton radioactivity in agreement with the experimental results of Mukha et al. for 94 Ag.

Study of particle size distribution and formation mechanism of radioactive aerosols generated in high-energy neutron fields

CERN Document Server

Endo, A; Noguchi, H; Tanaka, S; Iida, T; Furuichi, S; Kanda, Y; Oki, Y

2003-01-01

The size distributions of sup 3 sup 8 Cl, sup 3 sup 9 Cl, sup 8 sup 2 Br and sup 8 sup 4 Br aerosols generated by irradiations of argon and krypton gases containing di-octyl phthalate (DOP) aerosols with 45 MeV and 65 MeV quasi-monoenergetic neutrons were measured in order to study the formation mechanism of radioactive particles in high energy radiation fields. The effects of the size distribution of the radioactive aerosols on the size of the added DOP aerosols, the energy of the neutrons and the kinds of nuclides were studied. The observed size distributions of the radioactive particles were explained by attachment of the radioactive atoms generated by the neutron-induced reactions to the DOP aerosols. (author)

Mobility of radioactive colloidal particles in groundwater

International Nuclear Information System (INIS)

Nuttall, H.E.; Long, R.L.

1993-01-01

Radiocolloids are a major factor in the rapid migration of radioactive waste in groundwater. For at least two Los Alamos National Laboratory (LANL) sites, researchers have shown that groundwater colloidal particles were responsible for the rapid transport of radioactive waste material in groundwater. On an international scale, a review of reported field observations, laboratory column studies, and carefully collected field samples provides compelling evidence that colloidal particles enhance both radioactive and toxic waste migration. The objective of this project is to understand and predict colloid-contaminant migration through fundamental mathematical models, water sampling, and laboratory experiments and use this information to develop an effective and scientifically based colloid immobilization strategy. The article focuses on solving the suspected radiocolloid transport problems at LANL's Mortandad Canyon site. (author) 6 figs., 5 tabs., 18 refs

Field testing an OREX reg-sign based open-quotes point of generationclose quotes low-level radioactive waste reduction program at FP ampersand L's St. Lucie Plant

International Nuclear Information System (INIS)

Payne, K.; Haynes, B.

1996-01-01

Nuclear power facilities, both commercial and government operated, generate material called Dry Active Waste (DAW). DAW is a by-product of maintenance and operation of the power systems which contain radioactive materials. DAW can be any material contaminated with radioactive particles as long as it is not a fluid, typically: paper, cardboard, wood, plastics, cloth, and any other solid which is contaminated and determined to be dry. DAW is generated when any material is exposed to loose radioactive particles and subsequently becomes contaminated. In the United States, once a material is contaminated it must be treated as radioactive waste and disposed of in accordance with the requirements of Title 10 of the Code of Federal Regulations. Problems facing all commercial and non-commercial nuclear facilities are escalating costs of processing DAW and volumetric reduction of the DAW generated. Currently, approximately 85% of all DAW generated at a typical facility is comprised of anti-contamination clothing and protective barrier materials. Facilities that generate low-level radioactive waste need to dramatically reduce their waste volumes. This curtailment is required for several reasons: the number of radioactive waste repositories now accepting new waste is limited; the current cost of burial at an operating dump site is significant. Costs can be as high as $4,000 for a single 55 gallon drum; the cost of burial is constantly increasing; onsite storage of low-level radioactive waste is costly and results in a burial fee at plant decommissioning

Radiochemical assay for determination of dihydropyrimidinase activity using reversed-phase high-performance liquid chromatography

NARCIS (Netherlands)

van Kuilenburg, A. B.; van Lenthe, H.; van Gennip, A. H.

1999-01-01

A radiochemical assay was developed to measure the activity of dihydropyrimidinase (DHP) in human liver homogenates. The method is based on the separation of radiolabeled dihydrouracil from N-carbamyl-beta-alanine by HPLC with on-line detection of radioactivity combined with detection of 14CO2 by

Selection of optimal treatment procedures for non-standard radioactive waste arising from decommissioning of NPP after accident

Energy Technology Data Exchange (ETDEWEB)

Strážovec, Roman, E-mail: strazovec.roman@javys.sk [Institute of Nuclear and Physical Engineering, Slovak University of Technology in Bratislava, Ilkovičova 3, 812 19 Bratislava (Slovakia); JAVYS, a.s., Tomášikova 22, 821 02 Bratislava (Slovakia); Hrnčíř, Tomáš [DECOM, a.s., Sibírska 1, 917 01 Trnava (Slovakia); Lištjak, Martin [Institute of Nuclear and Physical Engineering, Slovak University of Technology in Bratislava, Ilkovičova 3, 812 19 Bratislava (Slovakia); VUJE, a.s., Okružná 5, 918 64 Trnava (Slovakia); Nečas, Vladimír [Institute of Nuclear and Physical Engineering, Slovak University of Technology in Bratislava, Ilkovičova 3, 812 19 Bratislava (Slovakia)

2016-05-15

The decommissioning of nuclear power plants is becoming a standard industrial activity where the optimization processes of partial activities are inevitable mainly for technical and economic reasons. In Slovakia, the decommissioning of A1 NPP is very specific case because A1 NPP is rare type of NPP (prototype) and furthermore its operation was affected by the accident. A large number of specific non-standard radioactive waste, such as long-time storage cases (hereinafter LSC), that is not usually present within the decommissioning projects of NPP with a regular termination of operation, represent one of the significant consequences of the accident and issues arisen from follow-up activities. The presented article describes the proposal of processing and conditioning of non-standard radioactive waste (such as LSC), together with description of methodology applied in the proposal for update of waste acceptance criteria for the processing and conditioning of radioactive waste (hereinafter RAW) within Bohunice Radioactive waste Treatment and Conditioning Centre (hereinafter RWTC). The results of performed detailed analysis are summarized into new waste acceptance criteria for technological lines keeping in mind safety principles and requirements for protection of operating personnel, the public and the environment.

[Exposure degree of important non-target arthropods to Cry2Aa in Bt rice fields].

Science.gov (United States)

Zhang, Qing-Ling; Li, Yun-He; Hua, Hong-Xia; Yang, Chang-Ju; Wu, Hong-Jin; Peng, Yu-Fa

2013-06-01

Based on the principle of "risk = hazard x exposure", the selected representative nontarget organisms in the assessment of the potential effects of insect-resistant genetically modified (GM) crops on non-target arthropods in laboratory are generally the arthropod species highly exposed to the insecticidal proteins expressed by the GM crops in farmland ecosystem. In order to understand the exposure degree of the important arthropod species to Cry proteins in Bt rice fields, and to select the appropriate non-target arthropods in the risk assessment of insect-resistant GM crops, the enzyme-linked immunosorbent assay (ELISA) was conducted to measure the Cry2Aa protein concentration in the arthropods collected from the cry2Aa rice fields at different rice growth stages. The results showed that there was a significant difference in the Cry2Aa content protein concentration in different arthropod species. Some species did not contain Cry2Aa protein, while some species contained larger amounts of Cry2Aa protein. Relative to the arthropods colleted after rice anthesis, the arthropods colleted in rice anthesis contained relative higher concentrations of Cry2Aa protein, especially for the predacious arthropods. No Cry proteins were detected in parasitic arthropods. This study provided references for the laboratory assessment of the effects of GM rice on nontarget arthropods.

Non-minimally coupled tachyon field in teleparallel gravity

Energy Technology Data Exchange (ETDEWEB)

Fazlpour, Behnaz [Department of Physics, Babol Branch, Islamic Azad University, Shariati Street, Babol (Iran, Islamic Republic of); Banijamali, Ali, E-mail: b.fazlpour@umz.ac.ir, E-mail: a.banijamali@nit.ac.ir [Department of Basic Sciences, Babol University of Technology, Shariati Street, Babol (Iran, Islamic Republic of)

2015-04-01

We perform a full investigation on dynamics of a new dark energy model in which the four-derivative of a non-canonical scalar field (tachyon) is non-minimally coupled to the vector torsion. Our analysis is done in the framework of teleparallel equivalent of general relativity which is based on torsion instead of curvature. We show that in our model there exists a late-time scaling attractor (point P{sub 4}), corresponding to an accelerating universe with the property that dark energy and dark matter densities are of the same order. Such a point can help to alleviate the cosmological coincidence problem. Existence of this point is the most significant difference between our model and another model in which a canonical scalar field (quintessence) is used instead of tachyon field.

Non-minimally coupled tachyon field in teleparallel gravity

International Nuclear Information System (INIS)

Fazlpour, Behnaz; Banijamali, Ali

2015-01-01

We perform a full investigation on dynamics of a new dark energy model in which the four-derivative of a non-canonical scalar field (tachyon) is non-minimally coupled to the vector torsion. Our analysis is done in the framework of teleparallel equivalent of general relativity which is based on torsion instead of curvature. We show that in our model there exists a late-time scaling attractor (point P 4 ), corresponding to an accelerating universe with the property that dark energy and dark matter densities are of the same order. Such a point can help to alleviate the cosmological coincidence problem. Existence of this point is the most significant difference between our model and another model in which a canonical scalar field (quintessence) is used instead of tachyon field

Chemistry and radioactivity: a century after Marie Curie

International Nuclear Information System (INIS)

Guillaumont, R.

2011-01-01

Coupling chemistry and radioactivity has led to radiochemistry, the part of chemistry dealing with the behaviour of radioactive materials. Many activities are of concern, as well in basic research as in the fields of health and energy. They call researches going from the study of the extremely diluted radioactive material (environment) until that of the most man-made radioactive material ever produced (spent nuclear fuel from reactors). When radiochemistry is not the mirror of the traditional chemistry, it uses in radioactive surroundings its own methods based on the measurement of the emitted rays. Radiochemistry will have in the next decades a major input to prepare the nuclear energy of the future. (author)

Field-Deployable Reverse Transcription-Insulated Isothermal PCR (RT-iiPCR) Assay for Rapid and Sensitive Detection of Foot-and-Mouth Disease Virus.

Science.gov (United States)

Ambagala, A; Fisher, M; Goolia, M; Nfon, C; Furukawa-Stoffer, T; Ortega Polo, R; Lung, O

2017-10-01

Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals, which can decimate the livestock industry and economy of countries previously free of this disease. Rapid detection of foot-and-mouth disease virus (FMDV) is critical to containing an FMD outbreak. Availability of a rapid, highly sensitive and specific, yet simple and field-deployable assay would support local decision-making during an FMDV outbreak. Here we report validation of a novel reverse transcription-insulated isothermal PCR (RT-iiPCR) assay that can be performed on a commercially available, compact and portable POCKIT ™ analyser that automatically analyses data and displays '+' or '-' results. The FMDV RT-iiPCR assay targets the 3D region of the FMDV genome and was capable of detecting 9 copies of in vitro-transcribed RNA standard with 95% confidence. It accurately identified 63 FMDV strains belonging to all seven serotypes and showed no cross-reactivity with viruses causing similar clinical diseases in cloven-hoofed animals. The assay was able to identify FMDV RNA in multiple sample types including oral, nasal and lesion swabs, epithelial tissue suspensions, vesicular and oral fluid samples, even before the appearance of clinical signs. Clinical sensitivity of the assay was comparable or slightly higher than the laboratory-based real-time RT-PCR assay in use. The assay was able to detect FMDV RNA in vesicular fluid samples without nucleic acid extraction. For RNA extraction from more complex sample types, a commercially available taco ™ mini transportable magnetic bead-based, automated extraction system was used. This assay provides a potentially useful field-deployable diagnostic tool for rapid detection of FMDV in an outbreak in FMD-free countries or for routine diagnostics in endemic countries with less structured laboratory systems. © 2016 Her Majesty the Queen in Right of Canada.

Degradation Analysis of Field-Exposed Photovoltaic Modules with Non-Fluoropolymer-Based Backsheets

Energy Technology Data Exchange (ETDEWEB)

Kempe, Michael D [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Fairbrother, Andrew [National Institute of Standards and Technology (NIST); Julien, Scott [Northeastern University; Wan, Kai-Tak [Northeastern University; Ji, Liang [Underwriters Laboratory; Boyce, Kenneth [Underwriters Laboratory; Merzlic, Sebastien [Arkema; Lefebvre, Amy [Arkema; O' Brien, Greg [Arkema; Wang, Yu [Case Western Reserve University; Bruckman, Laura [Case Western Reserve University; French, Roger [Case Western Reserve University; Gu, Xiaohong [National Institute of Standards and Technology (NIST)

2017-08-23

The selection of polymeric materials utilized in photovoltaic (PV) modules has changed relatively little since the inception of the PV industry, with ethylene-vinyl acetate (EVA), polyethylene terephthalate (PET), and fluoropolymer-based laminates being the most widely adopted primary components of the encapsulant and backsheet materials. The backsheet must serve to electrically insulate the solar cells and protect them from the effects of weathering. Due to continued downward pressure on cost, other polymeric materials are being formulated to withstand outdoor exposure for use in backsheets to replace either the PET film, the fluoropoymer film, or both. Because of their relatively recent deployment, less is known about their reliability and if they are durable enough to fulfill the greater than or equal to 25 year warranties of current PV modules. This work presents a degradation analysis of field-exposed modules with polyamide- and polyester-based backsheets. Modules were exposed for up to five years in different geographic locations: USA (Maryland, Ohio), China, and Italy. Surface and cross-sectional analysis included visual inspection, colorimetry, glossimetry, and Fourier-transform infrared spectroscopy. Each module experienced different types of degradation depending on the exposure site, even for the same material and module brand. For instance, the polyamide-based backsheet experienced hairline cracking and greater yellowing and chemical changes in China (Changsu, humid subtropical climate), while in Italy (Rome, hot-summer Mediterranean climate) it underwent macroscopic cracking and greater losses in gloss. Spectroscopic studies have permitted identification of degradation products and changes in polymer structure over time. Comparisons are made to fielded modules with fluoropolymer-based backsheets, as well as backsheet materials in accelerated laboratory exposures. Implications for qualification testing and service life prediction of the non-fluoropolymer-based

Degradation analysis of field-exposed photovoltaic modules with non-fluoropolymer-based backsheets

Science.gov (United States)

Fairbrother, Andrew; Julien, Scott; Wan, Kai-Tak; Ji, Liang; Boyce, Kenneth; Merzlic, Sebastien; Lefebvre, Amy; O'Brien, Greg; Wang, Yu; Bruckman, Laura; French, Roger; Kempe, Michael; Gu, Xiaohong

2017-08-01

The selection of polymeric materials utilized in photovoltaic (PV) modules has changed relatively little since the inception of the PV industry, with ethylene-vinyl acetate (EVA), polyethylene terephthalate (PET), and fluoropolymer-based laminates being the most widely adopted primary components of the encapsulant and backsheet materials. The backsheet must serve to electrically insulate the solar cells and protect them from the effects of weathering. Due to continued downward pressure on cost, other polymeric materials are being formulated to withstand outdoor exposure for use in backsheets to replace either the PET film, the fluoropoymer film, or both. Because of their relatively recent deployment, less is known about their reliability and if they are durable enough to fulfill the >=25 year warranties of current PV modules. This work presents a degradation analysis of field-exposed modules with polyamide- and polyester-based backsheets. Modules were exposed for up to five years in different geographic locations: USA (Maryland, Ohio), China, and Italy. Surface and cross-sectional analysis included visual inspection, colorimetry, glossimetry, and Fourier-transform infrared spectroscopy. Each module experienced different types of degradation depending on the exposure site, even for the same material and module brand. For instance, the polyamide-based backsheet experienced hairline cracking and greater yellowing and chemical changes in China (Changsu, humid subtropical climate), while in Italy (Rome, hot-summer Mediterranean climate) it underwent macroscopic cracking and greater losses in gloss. Spectroscopic studies have permitted identification of degradation products and changes in polymer structure over time. Comparisons are made to fielded modules with fluoropolymer-based backsheets, as well as backsheet materials in accelerated laboratory exposures. Implications for qualification testing and service life prediction of the non-fluoropolymer-based backsheets are

Site Study Plan for background environmental radioactivity, Deaf Smith County site, Texas: Environmental Field Program: Preliminary draft

International Nuclear Information System (INIS)

1987-06-01

The Background Environmental Radioactivity Site Study Plan describes a field program consisting of an initial radiological survey and a radiological sampling program. The field program includes measurement of direct radiation and collection and analysis of background radioactivity samples of air, precipitation, soil, water, milk, pasture grass, food crops, meat, poultry, game, and eggs. The plan describes for each study: the need for the study, the study design, data management and use, schedule of proposed activities, and quality assurance requirements. These studies will provide data needed to satisfy requirements contained in, or derived from, the Salt Repository Project (SRP) Requirements Document. 50 refs., 11 figs., 7 tabs

A simple method for the verification of clearance levels for non-radioactive solid waste

International Nuclear Information System (INIS)

Holland, B.

1997-01-01

ANSTO's radiopharmaceutical production laboratories generate 25 m 3 of solid waste per month. Most of this waste is not radioactive. Up until recently the non-radioactive waste was cleared from the controlled area and stored for 10 halflives prior to disposal as normal solid refuse. To eliminate the storage and ''double handling'' of the large quantities of non-radioactive waste a simple clearance method was devised to allow direct disposal. This paper describes how clearance levels were determined. Here the term ''clearance level'' is used as a general term for the release of material regardless of whether it was previously subject to regulatory control. This contrasts with the IAEA definition of a clearance level and highlights a potential problem with the implementation of exemption levels to keep material out of regulatory control and the use of clearance levels to allow removal of materials from regulatory control. Several common hand held contamination monitors were tested to determine their limits of detection and ability to meet these clearance levels. The clearance method includes waste segregation and size limitation features to ensure the waste is monitored in a consistent manner, compatible with the limits of detection. The clearance levels achieved were subsequently found to be compatible with some of the unconditional clearance levels in IAEA-TECDOC-855 and the measurement method also meets the required features of that document. The ANSTO non-radioactive waste clearance system has been in operation for more than 12 months and has proved simple and effective to operate. Approximately 12m 3 of the solid waste is now been treated directly as normal solid refuse. This paper describes the ANSTO clearance system, the contamination monitor tests and details practical problems associated with the direct monitoring of solid waste, including averaging of the activity in the package. The paper also briefly highlights the potential problem with the use of

Management of liquid radioactive waste from non-power applications at MosNPO 'Radon'

International Nuclear Information System (INIS)

Karlin, Yu.; Barinov, A.; Volkov, A.; Dmitriev, S.; Iljin, V.; Savkin, A.; Sobolev, I.; Flit, V.

2001-01-01

MosNPO 'Radon', founded in 1961, is an enterprise intended for the collecting, transportation, treatment, conditioning and disposal of radioactive waste formed outside of the nuclear fuel cycle, in the central part of Russia. Besides the main activity, MosNPO 'Radon' carries out a lot of research and design efforts in the field of management with solid radioactive waste (SRW) and liquid radioactive waste (LRW). Up to 10% LRW, being formed at Zagorsk branch of MosNPO 'Radon', are directed to the cementation without any concentrating. These are mainly radioactive waters with salt content more than 20 g/l. The rest LRW are concentrated in stationary and mobile installations. Concentrates (regenerates of ion-exchange filters, brine and spent sorbents) are also directed to the cementation. The cleaned waters (according to MosNPO 'Radon' radiation safety norms) are dropped out into the sewage. The cement compound, obtained on the base of LRW, is used for filling cavities in SRW tanks and metal barrels, which are used as packing of radioactive waste. The waters of surface flow are not LRW, as the common contents of radionuclides in these waters more often are less than 1 Bk/l. The technologies for the management with these waters at MosNPO 'Radon' are described in the paper. The experience of MosNPO 'Radon' on LRW cleaning-up at other organizations is described in the paper. For the realization of such works MosNPO 'Radon' has a mobile installation 'ECO-2' and modular water-cleaning complexes 'Aqua-Express' (it is located at Zagorsk branch of MosNPO 'Radon') and 'ECO-3M' (it is located at GMP 'Zvezdochka', in Severodvinsk, for cleaning radioactive waters formed during repairs of atomic submarines). Productivity of any installations by the cleaned water is from 0,2 m 3 /h up to 1 m 3 /h, depending on the LRW composition. The concentration degree of LRW is not less than 10, but more often is from 30 up to 100. Development of perspective technologies and elaboration of new

Performance validation of commercially available mobile waste-assay systems: Preliminary report

International Nuclear Information System (INIS)

Schanfein, M.; Bonner, C.; Maez, R.

1997-01-01

Prior to disposal, nuclear waste must be accurately characterized to identify and quantify the radioactive content to reduce the radioactive hazard to the public. Validation of the waste-assay systems' performance is critical for establishing the credibility of the assay results for storage and disposal purposes. Canberra Nuclear has evaluated regulations worldwide and identified standard, modular, neutron- and gamma-waste-assay systems that can be used to characterize a large portion of existing and newly generated transuranic (TRU) and low-level waste. Before making claims of guaranteeing any system's performance for specific waste types, the standardized systems' performance be evaluated. 7 figs., 11 tabs

Performance validation of commercially available mobile waste-assay systems: Preliminary report

Energy Technology Data Exchange (ETDEWEB)

Schanfein, M.; Bonner, C.; Maez, R. [Los Alamos National Lab., NM (United States)] [and others

1997-11-01

Prior to disposal, nuclear waste must be accurately characterized to identify and quantify the radioactive content to reduce the radioactive hazard to the public. Validation of the waste-assay systems` performance is critical for establishing the credibility of the assay results for storage and disposal purposes. Canberra Nuclear has evaluated regulations worldwide and identified standard, modular, neutron- and gamma-waste-assay systems that can be used to characterize a large portion of existing and newly generated transuranic (TRU) and low-level waste. Before making claims of guaranteeing any system`s performance for specific waste types, the standardized systems` performance be evaluated. 7 figs., 11 tabs.

Development of a toxR-based loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus

Directory of Open Access Journals (Sweden)

Ge Beilei

2010-02-01

Full Text Available Abstract Background Vibrio parahaemolyticus is a leading cause of seafood-related bacterial gastroenteritis and outbreaks worldwide. Sensitive and specific detection methods are needed to better control V. parahaemolyticus infections. This study aimed at developing a highly specific and sensitive loop-mediated isothermal amplification (LAMP assay for detecting V. parahaemolyticus in oysters. A set of five LAMP primers, two outer, two inner, and one loop were designed based on the published V. parahaemolyticus toxR sequence. Specificity of the assay was evaluated using a panel of 36 V. parahaemolyticus and 39 other strains. The assay sensitivity was determined using serial dilutions of V. parahaemolyticus ATCC 27969 culture ranging from 108 CFU/ml to extinction. The assay was also tested in experimentally inoculated oyster samples. Results The toxR-based LAMP assay was able to specifically detect all of the 36 V. parahaemolyticus strains without amplification from 39 other strains. The detection limit was 47-470 cells per reaction in pure culture, up to 100-fold more sensitive than that of toxR-PCR. When applied in spiked oysters, the assay was able to detect 1.1 × 105 V. parahaemolyticus cells per gram of oyster without enrichment, up to 100-fold more sensitive than that of toxR-PCR. Standard curves generated for detecting V. parahaemolyticus in both pure culture and spiked oyster samples showed good linear relationship between cell numbers and the fluorescence or turbidity signals. Conclusions The toxR-based LAMP assay developed in this study was sensitive, specific, and quantitative, holding great potential for future field detection of V. parahaemolyticus in raw oysters.

U Y 105 standard use of non sealed radioactive sources in nuclear medicine: approve for Industry energy and Mining Ministry 28/6/2002 Resolution

International Nuclear Information System (INIS)

2002-01-01

Establish minimal requirements radiological safety for use non sealed radioactive sources in nuclear medicine.The present standard is used in operation or nuclear medicine practices using non sealed radioactive sources with diagnostic and therapeutic purposes in vivo and in vitro

Recombinant immunoblot assay reaction patterns and hepatitis C virus RNA in blood donors and non-A, non-B hepatitis patients

NARCIS (Netherlands)

Bresters, D.; Zaaijer, H. L.; Cuypers, H. T.; Reesink, H. W.; Winkel, I. N.; van Exel-Oehlers, P. J.; van Drimmelen, A. A.; Jansen, P. L.; van der Poel, C. L.; Lelie, P. N.

1993-01-01

To establish the value of the second-generation recombinant immunoblot assay (RIBA-2) and cDNA polymerase chain reaction (cDNA PCR) for confirmation of hepatitis C virus (HCV) infection, anti-HCV reaction patterns and the presence of HCV RNA were examined in 610 blood donors and 255 non-A, non-B

Unveiling a spinor field classification with non-Abelian gauge symmetries

Science.gov (United States)

Fabbri, Luca; da Rocha, Roldão

2018-05-01

A spinor fields classification with non-Abelian gauge symmetries is introduced, generalizing the U(1) gauge symmetries-based Lounesto's classification. Here, a more general classification, contrary to the Lounesto's one, encompasses spinor multiplets, corresponding to non-Abelian gauge fields. The particular case of SU(2) gauge symmetry, encompassing electroweak and electromagnetic conserved charges, is then implemented by a non-Abelian spinor classification, now involving 14 mixed classes of spinor doublets. A richer flagpole, dipole, and flag-dipole structure naturally descends from this general classification. The Lounesto's classification of spinors is shown to arise as a Pauli's singlet, into this more general classification.

Test plan for Digface Chemical and Radiation Assay System

International Nuclear Information System (INIS)

Akers, D.W.

1993-07-01

The Digface Chemical and Radiation Assay System (CRAS) Project will develop a sensor using Prompt Gamma Neutron Activation Analysis (PGNAA) that can detect the present of hazardous chemicals and radioactive materials. The CRAS is being designed for in situ assay of closed drums and contaminated soils for gamma-ray emitting radionuclides and hazardous elements. The CRAS is based upon the use of 252 Cf PGNAA with a germanium gamma-ray spectrometer as the analyzer. Tasks being performed include determining detection limits for a number of hazardous chemicals and assessing matrix and transmission effects through soil. Initial analyses suggest that the technique is applicable to a number of hazardous materials such as trichloroethane and carbon tetrachloride

Assay of /sup 32/Si by liquid scintillation counting

Energy Technology Data Exchange (ETDEWEB)

Cumming, J B [Brookhaven National Lab., Upton, NY (USA)

1983-10-03

Application of the radioactivity of /sup 32/Si to problems of interest in geo- and cosmo-chemistry has been hampered by uncertainties in the half-life of this nuclide. A procedure for the stimulation assay of /sup 32/Si and its /sup 32/P daughter utilizing a liquid scintillation detector in association with a pulse height analyzer is described. The results indicate that /sup 32/Si can be assayed to an accuracy of a few percent by liquid scintillation counting techniques which do not require the preparation of an organic Si derivative. Combination of the mean specific activity with the /sup 32/Si abundance determined by accelerator-based mass spectrometry gave the reported 101+-18 year half-life.

The influence of organic materials on the near field of an intermediate level radioactive waste repository

International Nuclear Information System (INIS)

Wilkins, J.D.

1988-01-01

The influence of organic materials which are present in some intermediate level wastes on the chemistry of the near field of a radioactive waste repository is discussed. Particular attention is given to the possible formation of water soluble complexing agents as a result of the radiation field and chemical conditions. The present state of the research is reviewed. (author)

Performance Values for Non-Destructive Assay (NDA) Technique Applied to Wastes: Evaluation by the ESARDA NDA Working Group

International Nuclear Information System (INIS)

Rackham, Jamie; Weber, Anne-Laure; Chard, Patrick

2012-01-01

The first evaluation of NDA performance values was undertaken by the ESARDA Working Group for Standards and Non Destructive Assay Techniques and was published in 1993. Almost ten years later in 2002 the Working Group reviewed those values and reported on improvements in performance values and new measurement techniques that had emerged since the original assessment. The 2002 evaluation of NDA performance values did not include waste measurements (although these had been incorporated into the 1993 exercise), because although the same measurement techniques are generally applied, the performance is significantly different compared to the assay of conventional Safeguarded special nuclear material. It was therefore considered more appropriate to perform a separate evaluation of performance values for waste assay. Waste assay is becoming increasingly important within the Safeguards community, particularly since the implementation of the Additional Protocol, which calls for declaration of plutonium and HEU bearing waste in addition to information on existing declared material or facilities. Improvements in the measurement performance in recent years, in particular the accuracy, mean that special nuclear materials can now be accounted for in wastes with greater certainty. This paper presents an evaluation of performance values for the NDA techniques in common usage for the assay of waste containing special nuclear material. The main topics covered by the document are: 1- Techniques for plutonium bearing solid wastes 2- Techniques for uranium bearing solid wastes 3 - Techniques for assay of fissile material in spent fuel wastes. Originally it was intended to include performance values for measurements of uranium and plutonium in liquid wastes; however, as no performance data for liquid waste measurements was obtained it was decided to exclude liquid wastes from this report. This issue of the performance values for waste assay has been evaluated and discussed by the ESARDA

Performance Values for Non-Destructive Assay (NDA) Technique Applied to Wastes: Evaluation by the ESARDA NDA Working Group

Energy Technology Data Exchange (ETDEWEB)

Rackham, Jamie [Babcock International Group, Sellafield, Seascale, Cumbria, (United Kingdom); Weber, Anne-Laure [Institut de Radioprotection et de Surete Nucleaire Fontenay-Aux-Roses (France); Chard, Patrick [Canberra, Forss Business and Technology park, Thurso, Caithness (United Kingdom)

2012-12-15

The first evaluation of NDA performance values was undertaken by the ESARDA Working Group for Standards and Non Destructive Assay Techniques and was published in 1993. Almost ten years later in 2002 the Working Group reviewed those values and reported on improvements in performance values and new measurement techniques that had emerged since the original assessment. The 2002 evaluation of NDA performance values did not include waste measurements (although these had been incorporated into the 1993 exercise), because although the same measurement techniques are generally applied, the performance is significantly different compared to the assay of conventional Safeguarded special nuclear material. It was therefore considered more appropriate to perform a separate evaluation of performance values for waste assay. Waste assay is becoming increasingly important within the Safeguards community, particularly since the implementation of the Additional Protocol, which calls for declaration of plutonium and HEU bearing waste in addition to information on existing declared material or facilities. Improvements in the measurement performance in recent years, in particular the accuracy, mean that special nuclear materials can now be accounted for in wastes with greater certainty. This paper presents an evaluation of performance values for the NDA techniques in common usage for the assay of waste containing special nuclear material. The main topics covered by the document are: 1- Techniques for plutonium bearing solid wastes 2- Techniques for uranium bearing solid wastes 3 - Techniques for assay of fissile material in spent fuel wastes. Originally it was intended to include performance values for measurements of uranium and plutonium in liquid wastes; however, as no performance data for liquid waste measurements was obtained it was decided to exclude liquid wastes from this report. This issue of the performance values for waste assay has been evaluated and discussed by the ESARDA

Decontamination and radioactivity measurement on building surfaces related to dismantling of Japan power demonstration reactor (JPDR)

International Nuclear Information System (INIS)

Hatakeyama, Mutsuo; Tachibana, Mitsuo; Yanagihara, Satoshi

1997-12-01

In the final stage of dismantling activities for decommissioning a nuclear power plant, building structures have to be demolished to release the site for unrestricted use. Since building structures are generally made from massive reinforced concrete materials, it is not a rational way to treat all concrete materials arising from its demolition as radioactive waste. Segregation of radioactive parts from building structures is therefore indispensable. The rational procedures were studied for demolition of building structures by treating arising waste as non-radioactive materials, based on the concept established by Nuclear Safety Commission, then these were implemented in the following way by the JPDR dismantling demonstration project. Areas of the JPDR facilities are categorized into two groups : possibly contaminated areas, and possibly non-contaminated areas, based on the document of the reactor operation. Radioactivity on the building surfaces was then measured to confirm that the qualitative categorization is reasonable. After that, building surfaces were decontaminated in such a way that the contaminated layers were removed with enough margin to separate radioactive parts from non-radioactive building structures. Thought it might be possible to demolish the building structures by treating arising waste as non-radioactive materials, confirmation survey for radioactivity was conducted to show that there is no artificial radioactive nuclides produced by operation in the facility. This report describes the procedures studied on measurement of radioactivity and decontamination, and the results of its implementation in the JPDR dismantling demonstration project. (author)

Management of radioactive solid waste arisings from PFR reprocessing

International Nuclear Information System (INIS)

Allardice, R.H.; Hackney, S.; Bailey, G.; Bremner, W.; Lillyman, E.; Pugh, O.; Reekie, J.

1982-01-01

A description is given of the solid radioactive waste management facilities for dealing with the arisings from PFR reprocessing at the Dounreay Nuclear Power Development Establishment. Four major categories of solid waste are identified. The 'La Calhene' posting system for the transfer of active wastes which has been installed is discussed. The three new retrievable stores for high α#betta##betta#, high α low #betta##betta# and low α high #betta##betta# are described. The methods of waste categorisation by non-destructive assay techniques are outlined. Finally a review of operating experience with the facilities is presented. (U.K.)

Radioactive material inventory control at a waste characterization facility

International Nuclear Information System (INIS)

Yong, L.K.; Chapman, J.A.; Schultz, F.J.

1996-01-01

Due to the recent introduction of more stringent Department of Energy (DOE) regulations and requirements pertaining to nuclear and criticality safety, the control of radioactive material inventory has emerged as an important facet of operations at DOE nuclear facilities. In order to comply with nuclear safety regulations and nuclear criticality requirements, radioactive material inventories at each nuclear facility have to be maintained below limits specified for the facility in its safety authorization basis documentation. Exceeding these radioactive material limits constitutes a breach of the facility's nuclear and criticality safety envelope and could potentially result in an accident, cause a shut-down of the facility, and bring about imminent regulatory repercussions. The practice of maintaining control of radioactive material, especially sealed and unsealed sources, is commonplace and widely implemented; however, the requirement to track the entire radioactivity inventory at each nuclear facility for the purpose of ensuring nuclear safety is a new development. To meet the new requirements, the Applied Radiation Measurements Department at Oak Ridge National Laboratory (ORNL) has developed an information system, called the open-quotes Radioactive Material Inventory Systemclose quotes (RMIS), to track the radioactive material inventory at an ORNL facility, the Waste Examination and Assay Facility (WEAF). The operations at WEAF, which revolve around the nondestructive assay and nondestructive examination of waste and related research and development activities, results in an ever-changing radioactive material inventory. Waste packages and radioactive sources are constantly being brought in or taken out of the facility; hence, use of the RMIS is necessary to ensure that the radioactive material inventory limits are not exceeded

Radioactivity monitoring in Romania

International Nuclear Information System (INIS)

Alexandrescu, M.; Milu, C.

1996-01-01

Radioactivity monitoring in Romania is based on National Regulations for Radiation Protection enforced in 1976, on other environment protection laws enforced in the last years and on the recommendations of IAEA. Accordingly two systems of radioactive monitoring are to date operational in this field: the first one is the self-control of the radioactive emissions in the environment generated by the own nuclear activities (of nuclear units like the Cernavoda NPP, the Institute of Atomic Physics at Magurele-Bucharest, the Institute for Nuclear Research at Pitesti, the R Plant at Feldioara, Uranium mining units, etc.), while the other is based on two national agencies (the National Network of Environment Radiation Monitoring of the Ministry of Waters, Forests and Environment Protection and the Network of Radiation Hygiene Laboratories of the Health Ministry). The authors review and discuss the radiation protection legislation, the structure and the organizational operations of the national monitoring systems and the co-operation of the national monitoring systems with international authorities or programmes. 3 Figs., 1 tab., 11 refs

Regulation of naturally occurring radioactive materials in non-nuclear industries

International Nuclear Information System (INIS)

Scott, L.M.

1997-01-01

The volume and concentrations of naturally occurring radioactive material is large across a variety of industries commonly thought not to involve radioactive material. The regulation of naturally occurring radioactive material in the United States is in a state of flux. Inventory of naturally occurring radioactive materials is given, along with a range of concentrations. Current and proposed regulatory limits are presented. (author)

Radioactive materials

International Nuclear Information System (INIS)

Sugiura, Yoshio; Shimizu, Makoto.

1975-01-01

The problems of radioactivity in the ocean with marine life are various. Activities in this field, especially the measurements of the radioactivity in sea water and marine life are described. The works first started in Japan concerning nuclear weapon tests. Then the port call to Japan by U.S. nuclear-powered naval ships began. On the other hand, nuclear power generation is advancing with its discharge of warm water. The radioactive pollution of sea water, and hence the contamination of marine life are now major problems. Surveys of the sea areas concerned and study of the radioactivity intake by fishes and others are carried out extensively in Japan. (Mori, K.)

Processing radioactive wastes using membrane (UF/HF/RO) systems

International Nuclear Information System (INIS)

Doyle, R.D.

1988-01-01

Over the years many technologies have been utilized to process low level radioactive waste streams generated by the nuclear industry, including: demineralization, evaporation, reverse osmosis and filtration. In the early 1980's interest was generated in membrane technologies and their application to radioactive wastes. This interest was generated based on the capabilities shown by membrane systems in non-radioactive environments and the promise that reverse osmosis systems showed in early testing with radioactive wastes. Membrane technologies have developed from the early development of reverse osmosis system to also include specifically designed membranes for ultrafiltration and hyperfiltration applications

Radioactive ores from Agostinho field, Pocos de Caldas (MG), Brazil

International Nuclear Information System (INIS)

Fujimori, K.

1974-01-01

Aiming to characterize the radioactive minerals of Campo Agostinho, Pocos de Caldas - Brazil, the survey of all natural radiactive elements and their geochemical behaviors, decays and radioactive equilibrium was done. Several models of radioactive instability of the minerals or the radioactive samples were proposed to characterize the geochemistry and the mineralization of radioactive elements. The complete isotopic analysis of the elements was done by high resolution gamma spectrometry, using a Ge(Li) detector, at the temperature of liquid nitrogen, coupled to a multichannel analyser. The sample in radioactive equilibrium of Atomic Energy Comission of United States - A.S. n.6, NBL - New Brunswick Laboratory, was considered as standard sample. Fluorite, zircons, pirite, molibinite, rutile, anatase, niobophyllite, and in small ratio (bellow 0.1%) uranothorianite, coffinite, pyrochlore, monazite and apatite. (M.C.K.) [pt

Release process for non-real property containing residual radioactive material

International Nuclear Information System (INIS)

Ranek, N.L.; Chen, S.Y.; Kamboj, S.; Hensley, J.; Burns, D.; Fleming, R.; Warren, S.; Wallo, A.

1997-01-01

It is DOE's objective to operate its facilities and to conduct its activities so that radiation exposures to members of the public are maintained within acceptable limits and exposures to residual radioactive materials are controlled. To accomplish this, DOE has adopted Order DOE 5400.51 'Radiation Protection of the Public and the Environment', and will be promulgating IO CR Part 834 to codify and clarify the requirements of DOE 5400.5. Under both DOE 5400.5 and 10 CR Part 834, radioactively contaminated DOE property is prohibited from release unless specific actions have been completed prior to the release. This paper outlines a ten-step process that, if followed, will assist DOE Operations and contractor personnel in ensuring that the required actions established by Order DOE 5400.5 and 10 CR Part 834 have been appropriately completed prior to the release for reuse or recycle of non-real property (e.g., office furniture, computers, hand tools, machinery, vehicles and scrap metal). Following the process will assist in ensuring that radiological doses to the public from the released materials will meet applicable regulatory standards and be as low as reasonably achievable (ALARA)

Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays.

Science.gov (United States)

Fischer, Janine; Prosenc, Marc H; Wolff, Martin; Hort, Norbert; Willumeit, Regine; Feyerabend, Frank

2010-05-01

Magnesium (Mg) alloys are promising materials for the development of biodegradable implants. However, the current in vitro test procedures for cytotoxicity, cell viability and proliferation are not always suitable for this class of materials. In this paper we show that tetrazolium-salt-based assays, which are widely used in practice, are influenced by the corrosion products of Mg-based alloys. Corroded Mg converts tetrazolium salts to formazan, leading to a higher background and falsifying the results of cell viability. Tetrazolium-based assays are therefore not a useful tool for testing the cytotoxicity of Mg in static in vitro assays. Copyright (c) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Computer-determined assay time based on preset precision

International Nuclear Information System (INIS)

Foster, L.A.; Hagan, R.; Martin, E.R.; Wachter, J.R.; Bonner, C.A.; Malcom, J.E.

1994-01-01

Most current assay systems for special nuclear materials (SNM) operate on the principle of a fixed assay time which provides acceptable measurement precision without sacrificing the required throughput of the instrument. Waste items to be assayed for SNM content can contain a wide range of nuclear material. Counting all items for the same preset assay time results in a wide range of measurement precision and wastes time at the upper end of the calibration range. A short time sample taken at the beginning of the assay could optimize the analysis time on the basis of the required measurement precision. To illustrate the technique of automatically determining the assay time, measurements were made with a segmented gamma scanner at the Plutonium Facility of Los Alamos National Laboratory with the assay time for each segment determined by counting statistics in that segment. Segments with very little SNM were quickly determined to be below the lower limit of the measurement range and the measurement was stopped. Segments with significant SNM were optimally assays to the preset precision. With this method the total assay time for each item is determined by the desired preset precision. This report describes the precision-based algorithm and presents the results of measurements made to test its validity

Measurement of g factors of excited states in radioactive beams by the transient field technique: 132Te

International Nuclear Information System (INIS)

Benczer-Koller, N.; Kumbartzki, G.; Gurdal, G; Gross, Carl J; Krieger, B; Hatarik, Robert; O'Malley, Patrick; Pain, S. D.; Segen, L.; Baktash, Cyrus; Bingham, C. R.; Danchev, M.; Grzywacz, R.; Mazzocchi, C.

2008-01-01

The g factor of the 2 1 + state in 52 132 Te, E(2 1 + ) = 0.9739 MeV, r = 2.6 ps, was measured by the transient field technique applied to a radioactive beam. The development of an experimental approach necessary for work in radioactive beam environments is described. The result g = 0.28(15) agrees with the previous measurement by the recoil-in-vacuum technique, but here the sign of the g factor is measured as well

A model to determine the radiological implications of non-fixed radioactive contamination on the surfaces of packages and conveyances

International Nuclear Information System (INIS)

Hughes, J.S.; Warner Jones, S.M.; Lizot, M.T.; Perrin, M.L.; Thierfeld, S.; Schroedl, E.; Schwarz, G.; Rawl, R.; Munakata, M.; Hirose, M.

2004-01-01

The surfaces of packages and conveyances used to transport radioactive materials can sometimes become contaminated with radioactive material. This usually occurs as a result of the transfer of radioactive material from areas in which these packages and conveyances are handled. This contamination may subsequently be transferred to transport equipment, workers and to areas accessible to the public. This can represent a significant radiation safety issue that requires careful management. The current regulatory limits for non-fixed contamination on packages and conveyances have been in use for over 40 years, and are based on a simple exposure model. However, the bases on which these limits were derived have been subject to changes, as a result of successive revisions of international recommendations. In recognition of this need for a review and analysis of the current contamination limits an IAEA Co-ordinated Research Project (CRP) on the ''Radiological Aspects of Package and Conveyance Non-Fixed Contamination'' was initiated to review the scientific basis for the current regulatory limits for surface contamination. The CRP was also to develop guidance material for evaluating the radiological significance of surface contamination to workers and the public in light of state-of-the-art research, technical developments and current transport practices. The specific objectives of the work undertaken within this multi-national CRP were, in accordance with the terms of reference: To ensure that appropriate models exist for all package types including consideration of the aspects pertinent for assessing and revising a surface contamination model for transport. To collect - where possible - contamination, operational and dosimetric data to ensure modelling consistency. To use models for assessing the limitations and optimisation of radiation doses incurred in transport operations, and to consider preventive methods for package and conveyance contamination

Scientific co-operation with IAEA in the field of marine radioactivity

International Nuclear Information System (INIS)

Bologa, S.Alexandru

1998-01-01

A short presentation of the communication presented in the Seminar on public information on peaceful uses of nuclear energy, NUCInfo'98, is given. It is emphasized the formation of RMRI specialists, its scientific co-operations with IAEA between 1986-1998 and participations in related scientific events. In the domain of specialists' formation there are mentioned the participation of members of the Institute in the training courses on marine radioecology, in the regional course on strategies and methodologies for applied marine radioactivity and environmental isotope studies for the Black Sea, and in the inter-regional course devoted to determination of radionuclides in environmental samples, held in 1986, 1994 and 1997, respectively. In the field of research co-operation there are presented the achievements obtained in the framework of five programs dealing mainly with monitoring of marine water, sediment and biota radioactivity in Romanian sector of Black Sea. Also reported are the participations and the contributions in the international scientific events devoted to marine environmental studies and the contributions to international databases

Calibration method based on direct radioactivity measurement for radioactive gas monitoring instruments

International Nuclear Information System (INIS)

Yoshida, Makoto; Ohi, Yoshihiro; Chida, Tohru; Wu, Youyang.

1993-01-01

A calibration method for radioactive gas monitoring instruments was studied. In the method, gaseous radioactivity standards were provided on the basis of the direct radioactivity measurement by the diffusion-in long proportional counter method (DLPC method). The radioactivity concentration of the gas mixture through a monitoring instrument was determined by sampling the known volume of the gas mixture into the proportional counter used for the DLPC method. Since oxygen in the gas mixture decreased the counting efficiency in a proportional counter, the influence on calibration was experimentally estimated. It was not serious and able to be easily corrected. By the present method, the relation between radioactivity concentration and ionization current was determined for a gas-flow ionization chamber with 1.5 l effective volume. It showed good agreement with the results in other works. (author)

New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection.

Science.gov (United States)

Anumula, Kalyan Rao

2012-07-01

Assays were developed using the unique labeling chemistry of 2-aminobenzoic acid (2AA; anthranilic acid, AA) for measuring activities of both β1-4 galactosyltransferase (GalT-1) and α2-6 sialyltransferase (ST-6) by high-performance liquid chromatography (HPLC) with fluorescence detection (Anumula KR. 2006. Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates. Anal Biochem. 350:1-23). N-Acetylglucosamine (GlcNAc) and N-acetyllactosamine were used as acceptors and uridine diphosphate (UDP)-galactose and cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) as donors for GalT-1 and ST-6, respectively. Enzymatic products were labeled in situ with AA and were separated from the substrates on TSKgel Amide 80 column using normal-phase conditions. Enzyme units were determined from the peak areas by comparison with the concomitantly derivatized standards Gal-β1-4GlcNAc and NANA-α2-6 Gal-β1-4GlcNAc. Linearity (time and enzyme concentration), precision (intra- and interassay) and reproducibility for the assays were established. The assays were found to be useful in monitoring the enzyme activities during isolation and purification. The assays were highly sensitive and performed equal to or better than the traditional radioactive sugar-based measurements. The assay format can also be used for measuring the activity of other transferases, provided that the carbohydrate acceptors contain a reducing end for labeling. An assay for glycoprotein acceptors was developed using IgG. A short HPLC profiling method was developed for the separation of IgG glycans (biantennary G0, G1, G2, mono- and disialylated), which facilitated the determination of GalT-1 and ST-6 activities in a rapid manner. Furthermore, this profiling method should prove useful for monitoring the changes in IgG glycans in clinical settings.

Development of a database system for the management of non-treated radioactive waste

Energy Technology Data Exchange (ETDEWEB)

Pinto, Antônio Juscelino; Freire, Carolina Braccini; Cuccia, Valeria; Santos, Paulo de Oliveira; Seles, Sandro Rogério Novaes; Haucz, Maria Judite Afonso, E-mail: ajp@cdtn.br, E-mail: cbf@cdtn.br, E-mail: vc@cdtn.br, E-mail: pos@cdtn.br, E-mail: seless@cdtn.br, E-mail: hauczmj@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

2017-07-01

The radioactive waste produced by the research laboratories at CDTN/CNEN, Belo Horizonte, is stored in the Non-Treated Radwaste Storage (DRNT) until the treatment is performed. The information about the waste is registered and the data about the waste must to be easily retrievable and useful for all the staff involved. Nevertheless, it has been kept in an old Paradox database, which is now becoming outdated. Thus, to achieve this goal, a new Database System for the Non-treated Waste will be developed using Access® platform, improving the control and management of solid and liquid radioactive wastes stored in CDTN. The Database System consists of relational tables, forms and reports, preserving all available information. It must to ensure the control of the waste records and inventory. In addition, it will be possible to carry out queries and reports to facilitate the retrievement of the waste history and localization and the contents of the waste packages. The database will also be useful for grouping the waste with similar characteristics to identify the best type of treatment. The routine problems that may occur due to change of operators will be avoided. (author)

Development of a database system for the management of non-treated radioactive waste

International Nuclear Information System (INIS)

Pinto, Antônio Juscelino; Freire, Carolina Braccini; Cuccia, Valeria; Santos, Paulo de Oliveira; Seles, Sandro Rogério Novaes; Haucz, Maria Judite Afonso

2017-01-01

The radioactive waste produced by the research laboratories at CDTN/CNEN, Belo Horizonte, is stored in the Non-Treated Radwaste Storage (DRNT) until the treatment is performed. The information about the waste is registered and the data about the waste must to be easily retrievable and useful for all the staff involved. Nevertheless, it has been kept in an old Paradox database, which is now becoming outdated. Thus, to achieve this goal, a new Database System for the Non-treated Waste will be developed using Access® platform, improving the control and management of solid and liquid radioactive wastes stored in CDTN. The Database System consists of relational tables, forms and reports, preserving all available information. It must to ensure the control of the waste records and inventory. In addition, it will be possible to carry out queries and reports to facilitate the retrievement of the waste history and localization and the contents of the waste packages. The database will also be useful for grouping the waste with similar characteristics to identify the best type of treatment. The routine problems that may occur due to change of operators will be avoided. (author)

COWAM 2 : A European Contribution to the Improvement of Governance in the Field of Radioactive Waste Management

International Nuclear Information System (INIS)

Heriard Dubreuil, Gilles

2006-01-01

The quality of decision-making processes in radioactive waste management (RWM) was explored within the European 5th Framework Research Programme, through a Concerted Action project known as COWAM (Community Waste Management). COWAM (2000-2003) was established as a network, gathering European local communities, together with representatives of regulators, implementers and experts. The project made a major contribution to framing the governance of radioactive waste management by identifying key needs: early involvement of local stakeholders in the decision making processes on radioactive waste management; effective implementation of local democracy; access to expertise; influence of local actors on a defined national framework for radioactive waste management; and sustainable regional development of communities hosting radioactive waste management facilities. As a follow-on project from COWAM, COWAM 2 has been established within the European 6th Framework Research Programme. COWAM 2 started in January 2004 and will be completed at the end of 2006. The project format evolved from that of a network for collective reflection into a pluralistic research partnership, aimed at practical implementation. The involvement of stakeholders continues to be an essential element of the project, in terms of the quality, legitimacy and robustness of the results. Representatives of local communities and NGOs as well as institutional stakeholders contribute expertise on issues with which they are directly concerned, working in conjunction with specialists from various fields of study relevant to the governance of radioactive waste management. A stakeholder Steering Committee oversees the project. The objective of COWAM 2 is to contribute to a concrete improvement in RWM governance, by: better identifying and understanding societal expectations, needs and concerns as regards decision-making processes, notably at the local and regional levels, taking into account past and ongoing

COWAM 2 : A European Contribution to the Improvement of Governance in the Field of Radioactive Waste Management

Energy Technology Data Exchange (ETDEWEB)

Heriard Dubreuil, Gilles [MUTADIS, Paris (FR)] (and others)

2006-09-15

The quality of decision-making processes in radioactive waste management (RWM) was explored within the European 5th Framework Research Programme, through a Concerted Action project known as COWAM (Community Waste Management). COWAM (2000-2003) was established as a network, gathering European local communities, together with representatives of regulators, implementers and experts. The project made a major contribution to framing the governance of radioactive waste management by identifying key needs: early involvement of local stakeholders in the decision making processes on radioactive waste management; effective implementation of local democracy; access to expertise; influence of local actors on a defined national framework for radioactive waste management; and sustainable regional development of communities hosting radioactive waste management facilities. As a follow-on project from COWAM, COWAM 2 has been established within the European 6th Framework Research Programme. COWAM 2 started in January 2004 and will be completed at the end of 2006. The project format evolved from that of a network for collective reflection into a pluralistic research partnership, aimed at practical implementation. The involvement of stakeholders continues to be an essential element of the project, in terms of the quality, legitimacy and robustness of the results. Representatives of local communities and NGOs as well as institutional stakeholders contribute expertise on issues with which they are directly concerned, working in conjunction with specialists from various fields of study relevant to the governance of radioactive waste management. A stakeholder Steering Committee oversees the project. The objective of COWAM 2 is to contribute to a concrete improvement in RWM governance, by: better identifying and understanding societal expectations, needs and concerns as regards decision-making processes, notably at the local and regional levels, taking into account past and ongoing

Assay method and compositions

International Nuclear Information System (INIS)

1977-01-01

Methods are described for measuring catecholamine levels in human and animal body fluids and tissues using the catechol-O-methyl-transferase (COMT) radioassay. The assay involves incubating the biological sample with COMT and the tritiated methyl donor, S-adenosyl-L-methionine( 3 H)-methyl. The O-methylated ( 3 H) epinephrine and/or norepinephrine are extracted and oxidised to vanillin- 3 H which in turn is extracted and its radioactivity counted. When analysing dopamine levels the assay is extended by vanillin- 3 H and raising the pH of the aqueous periodate phase from which O-methylated ( 3 H) dopamine is extracted and counted. The assay may be modified depending on whether measurements of undifferentiated total endogenous catecholamine levels or differential analyses of the catecholamine levels are being performed. The sensitivity of the assay can be as low as 5 picograms for norepinephrine and epinephrine and 12 picograms for dopamine. The assemblance of the essential components of the assay into a kit for use in laboratories is also described. (U.K.)

Studies of hyperfine magnetic fields in transition metals by radioactive ion implantation

International Nuclear Information System (INIS)

Kawase, Yoichi; Uehara, Shin-ichi; Nasu, Saburo; Ni Xinbo.

1994-01-01

In order to investigate hyperfine magnetic fields in transition metals by a time-differential perturbed angular correlation (TDPAC) technique, radioactive probes of 140 Cs obtained by KUR-ISOL have been implanted on transition metals of Fe, Ni and Co. Lamor precessions of 140 Ce used as a probe nucleus have been observed clearly and the hyperfine fields have been determined precisely corresponding to implanted sites in host metal. The irradiation effects caused by implantation have been examined by annealing the irradiated specimen at about 723 K. Some of the Lamor precessions have disappeared by the annealing. Discussions have been made on the occupied sites after implantation and the recovery process of induced damages by annealing. (author)

Regulations for the disposal of radioactive waste in the Konrad repository - 59105

International Nuclear Information System (INIS)

Jung, Hagen G.; Bandt, Gabriele

2012-01-01

In Germany low / medium level waste, which is classified here as radioactive waste with negligible heat generation, will be disposed of in the Konrad underground repository. The construction and the operation of this nuclear facility required authorization by different fields of law, i.e., by nuclear law, mining law and water law. Whereas the nuclear law considers solely radiological aspects, the relevant permit issued according to the water law considers the impact of radioactive as well as non-radioactive harmful substances. The Federal Office for Radiation Protection (BfS) as operator of the repository and permit holder has (a) to record the disposed of radioactive and non-radioactive harmful substances and (b) to balance them. To meet these requirements BfS has developed a concept, which led to a site specific solution. Threshold values were defined for recording and for balancing the harmful substances. It had to be verified that by disposal of radioactive waste packages according to these values an adverse effect on the near-surface groundwater can be excluded. The Lower Saxony Water Management, Coastal Protection and Nature Conservation Agency (NLWKN) as the responsible water law regulatory authority approved the operator's concept as appropriate to comply with the requirements of the Water Law Permit. Nonetheless, collateral clauses were imposed to assure this. (authors)

A highly scalable peptide-based assay system for proteomics.

Directory of Open Access Journals (Sweden)

Igor A Kozlov

Full Text Available We report a scalable and cost-effective technology for generating and screening high-complexity customizable peptide sets. The peptides are made as peptide-cDNA fusions by in vitro transcription/translation from pools of DNA templates generated by microarray-based synthesis. This approach enables large custom sets of peptides to be designed in silico, manufactured cost-effectively in parallel, and assayed efficiently in a multiplexed fashion. The utility of our peptide-cDNA fusion pools was demonstrated in two activity-based assays designed to discover protease and kinase substrates. In the protease assay, cleaved peptide substrates were separated from uncleaved and identified by digital sequencing of their cognate cDNAs. We screened the 3,011 amino acid HCV proteome for susceptibility to cleavage by the HCV NS3/4A protease and identified all 3 known trans cleavage sites with high specificity. In the kinase assay, peptide substrates phosphorylated by tyrosine kinases were captured and identified by sequencing of their cDNAs. We screened a pool of 3,243 peptides against Abl kinase and showed that phosphorylation events detected were specific and consistent with the known substrate preferences of Abl kinase. Our approach is scalable and adaptable to other protein-based assays.

Radioactivity nuclide identification based on BP and LM algorithm neural network

International Nuclear Information System (INIS)

Wang Jihong; Sun Jian; Wang Lianghou

2012-01-01

The paper provides the method which can identify radioactive nuclide based on the BP and LM algorithm neural network. Then, this paper compares the above-mentioned method with FR algorithm. Through the result of the Matlab simulation, the method of radioactivity nuclide identification based on the BP and LM algorithm neural network is superior to the FR algorithm. With the better effect and the higher accuracy, it will be the best choice. (authors)

Battery operated preconcentration-assisted lateral flow assay.

Science.gov (United States)

Kim, Cheonjung; Yoo, Yong Kyoung; Han, Sung Il; Lee, Junwoo; Lee, Dohwan; Lee, Kyungjae; Hwang, Kyo Seon; Lee, Kyu Hyoung; Chung, Seok; Lee, Jeong Hoon

2017-07-11

Paper-based analytical devices (e.g. lateral flow assays) are highly advantageous as portable diagnostic systems owing to their low costs and ease of use. Because of their low sensitivity and detection limits for biomolecules, these devices have several limitations in applications for real-field diagnosis. Here, we demonstrate a paper-based preconcentration enhanced lateral flow assay using a commercial β-hCG-based test. Utilizing a simple 9 V battery operation with a low power consumption of approximately 81 μW, we acquire a 25-fold preconcentration factor, demonstrating a clear sensitivity enhancement in the colorimetric lateral flow assay; consequently, clear colors are observed in a rapid kit test line, which cannot be monitored without preconcentration. This device can also facilitate a semi-quantitative platform using the saturation value and/or color intensity in both paper-based colorimetric assays and smartphone-based diagnostics.

Study of radiation dose from radioactive shipments to cargo handlers at O'Hare Field

International Nuclear Information System (INIS)

1975-06-01

The relationship between the amount of radioactive materials that pass through the cargo areas at O'Hare Field, Chicago, and the resulting radiation dose to the workers handling the packages was determined. Measurements of radiation exposure rates at various distances from the radioactive packages were made. Handling or exposure times for personnel during several unit operations were measured with a watch. These data were used to determine the body dose from the unit operation. In randomly selected cases workers were assigned TLD dosimeters in the form of a ring to be worn on the hand or in the form of a clip to be attached to the clothing for measurement of the hand or body dose from a unit operation. During each survey, several workers were given TLDs that were worn for the duration of the shift, and some workers, in addition, were given dosimeters to be worn for the duration of the study. Results showed that the workers who handled the radioactive packages did not receive any significant radiation exposure. (U.S.)

The importance of international cooperation in the field of high level radioactive waste management

International Nuclear Information System (INIS)

Isaacs, Thomas H.

1992-01-01

This paper discusses the importance of international collaboration in the field of radioactive waste management and points out how cooperation has benefited the U.S. civilian waste management program. The U.S. Department of Energy's Office of Civilian Radioactive Waste Management (OCRWM) oversees the handling, transportation, storage, and final deposition of high-level radioactive wastes for the U.S. commercial sector. Because OCRWM shares many of the same waste management concerns as various other countries with nuclear programs, and since one country's waste management program will ultimately have an impact on the waste management programs of other countries, it is clearly in the interest of all countries to work together in search of solutions to common waste management problems. To facilitate this. cooperation, OCRWM is a participating member of international organizations, such as the IAEA and the OECD/NEA. OCRWM further has in place several bilateral agreements with various individual countries and with the Commission of the European Communities (CEC). Other international waste management initiatives are also currently being considered. (author)

Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays

Science.gov (United States)

de Moraes, Marcela Cristina; Cardoso, Carmen Lucia; Seidl, Claudia; Moaddel, Ruin; Cass, Quezia Bezerra

2016-01-01

Affinity-based chromatography assays encompass the use of solid supports containing immobilized biological targets to monitor binding events in the isolation , identification and/or characterization of bioactive compounds. This powerful bioanalytical technique allows the screening of potential binders through fast analyses that can be directly performed using isolated substances or complex matrices. An overview of the recent researches in frontal and zonal affinity-based chromatography screening assays, which has been used as a tool in the identification and characterization of new anti-cancer agents, is discussed. In addition, a critical evaluation of the recently emerged ligands fishing assays in complex mixtures is also discussed. PMID:27306095

Reentrainment of radioactive nuclei from filters

International Nuclear Information System (INIS)

Dincklage, R.-D. von

1982-01-01

The possible relevance of atomic phenomena for the reentrainment of radioactive nuclei is discussed. The considerations are based on the coulombic fragmentation mechanism. Nuclei of potential interest in reprocessing technology are identified. Future experiments have been shown to be of definite need in this field. (author)

Beyond low-level activity: On a 'non-radioactive' gas mantle

Energy Technology Data Exchange (ETDEWEB)

Poljanc, Karin [Atominstitut der Osterreichischen Universitaeten, Vienna University of Technology, Stadionallee 2, 1020 Vienna (Austria); Steinhauser, Georg [Atominstitut der Osterreichischen Universitaeten, Vienna University of Technology, Stadionallee 2, 1020 Vienna (Austria)]. E-mail: georg.steinhauser@ati.ac.at; Sterba, Johannes H. [Atominstitut der Osterreichischen Universitaeten, Vienna University of Technology, Stadionallee 2, 1020 Vienna (Austria); Buchtela, Karl [Atominstitut der Osterreichischen Universitaeten, Vienna University of Technology, Stadionallee 2, 1020 Vienna (Austria); Bichler, Max [Atominstitut der Osterreichischen Universitaeten, Vienna University of Technology, Stadionallee 2, 1020 Vienna (Austria)

2007-03-01

Gas mantles for camping gas lanterns sometimes contain thorium compounds. During the last years, the use of thorium-free gas mantles has become more and more popular due to the avoidance of a radioactive heavy metal. We investigated a gas mantle type that is declared to be 'non-radioactive' and that can be bought in Austria at the moment. Methods used were Instrumental Neutron Activation Analysis (INAA), {gamma}-spectroscopy, and Liquid Scintillation Counting (LSC). We found massive thorium contents of up to 259 mg per gas mantle. Leaching experiments showed that only 0.4% of the Th but approximately 90% of the decay products of {sup 232}Th can be leached under conditions simulating sucking and chewing with human saliva. In this paper, the investigation of these gas mantles including the consideration of the environmental hazard caused by disposed mantles and the health hazard for unsuspecting consumers is presented and legal consequences are discussed for this fraud.

Proton radioactivity at non-collective prolate shape in high spin state of {sup 94}Ag

Energy Technology Data Exchange (ETDEWEB)

Aggarwal, Mamta, E-mail: mamta.a4@gmail.co [UM-DAE Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Mumbai 400 098 (India)

2010-10-11

We predict proton radioactivity and structural transitions in high spin state of an excited exotic nucleus near proton drip line in a theoretical framework and investigate the nature and the consequences of the structural transitions on separation energy as a function of temperature and spin. It reveals that the rotation of the excited exotic nucleus {sup 94}Ag at excitation energies around 6.7 MeV and angular momentum near 21h generates a rarely seen prolate non-collective shape and proton separation energy becomes negative which indicates proton radioactivity in agreement with the experimental results of Mukha et al. for {sup 94}Ag.

Monitoring system with integrated measuring sensors for radioactively contaminated iron and non-iron scrap metal (MerEN). Final report; Ueberwachungssystem mit integrierter Messsensorik fuer radioaktiv belastete Eisen- und Nichteisenschrotte (MerEN). Abschlussbericht

Energy Technology Data Exchange (ETDEWEB)

Celebic, Enis; Gentes, Sascha [Karlsruher Institut fuer Technologie (KIT), Karlsruhe (Germany). Inst. fuer Technologie und Management im Baubetrieb; Rutschmann, Michael; Goerisch, Uwe [Prof. Dr.-Ing. Uwe Goerisch GmbH Ingenieurbuero fuer Abfallwirtschaft, Karlsruhe (Germany); Wetzel, Ramona [Schrott Wetzel GmbH, Mannheim (Germany)

2015-08-15

Radioactive sources are used in the industry, in nuclear medicine, the military, as well as in research. Accidents and losses rarely occur, a proper and responsible handling of those sources provided. Radioactive sources represent a risk when divulged, moved, passed on without authorization or lost. Time and again, radioactive sources are found at scrap yards and metal processing facilities. The supervision of these radioactive materials is gaining importance in the light of the worldwide import and export of ferrous and non-ferrous scrap. The aim of the project was to develop a space monitoring system for radioactively contaminated ferrous and non-ferrous scrap, so it can be removed from the operating range and to protect staff. The monitoring system combines technical and application-specific requirements. As part of the research project, the system was designed based on the operational framework conditions, technical and economic possibilities, and the findings from the experimental phase. The prototype mainly consists of a mainframe computer, stationary and mobile detection units, and the data transfer technology. This has successfully been tested at a scrap yard. The effects of vibrations that occur on scrapyards were investigated. This was necessary to obtain functionality of the hardware. The experimental phase was carried out based on a pre-defined set-up. The aim was to test the individual scenarios, processing and logging of the date as well to interpret the test results. In the event of radioactive sources being found in discarded metal, a standard sequence of actions was designed to protect the yard's processes and its personnel against further radioactive damage. For the first time, active radiation monitoring was performed on scrap-processing machines and in the working range of mobile devices. With this, scrap yard operators will have the opportunity to detect radioactively contaminated material at an early stage and before radiation sources are

Clinical evaluation of the Abbott RealTime MTB Assay for direct detection of Mycobacterium tuberculosis-complex from respiratory and non-respiratory samples.

Science.gov (United States)

Hinić, Vladimira; Feuz, Kinga; Turan, Selda; Berini, Andrea; Frei, Reno; Pfeifer, Karin; Goldenberger, Daniel

2017-05-01

Rapid and reliable diagnosis is crucial for correct management of tuberculosis. The Abbott RealTime MTB Assay represents a novel qualitative real-time PCR assay for direct detection of M. tuberculosis-complex (MTB) DNA from respiratory samples. The test targets two highly conserved sequences, the multi-copy insertion element IS6110 and the protein antigen B (PAB) gene of MTB, allowing even the detection of IS6610-deficient strains. We evaluated this commercial diagnostic test by analyzing 200 respiratory and, for the first time, 87 non-respiratory clinical specimens from our tertiary care institution and compared its results to our IS6110-based in-house real-time PCR for MTB as well as MTB culture. Overall sensitivity for Abbott RealTime MTB was 100% (19/19) in smear positive and 87.5% (7/8) in smear negative specimens, while the specificity of the assay was 100% (260/260). For both non-respiratory smear positive and smear negative specimens Abbott RealTime MTB tests showed 100% (8/8) sensitivity and 100% (8/8) specificity. Cycle threshold (Ct) value analysis of 16 MTB positive samples showed a slightly higher Ct value of the Abbott RealTime MTB test compared to our in-house MTB assay (mean delta Ct = 2.55). In conclusion, the performance of the new Abbott RealTime MTB Assay was highly similar to culture and in-house MTB PCR. We document successful analysis of 87 non-respiratory samples with the highly automated Abbott RealTime MTB test with no inhibition observed. Copyright © 2017 Elsevier Ltd. All rights reserved.

Radiant-heat spray-calcination process for the solid fixation of radioactive waste. Part 1, Non-radioactive pilot unit

Energy Technology Data Exchange (ETDEWEB)

Allemann, R.T.; Johnson, B.M. Jr.

1960-11-14

The fixation of radioactive waste in a stable solid media by means of calcination of these aqueous solutions has been the subject of considerable-effort throughout the U. S. Atomic Energy Commission and by atomic energy organizations in other countries. Several methods of doing this on a continuous or semi-continuous basis have been devised, and a fev have been demonstrated to be feasible for the handling of non-radioactive, or low-activity, simulated wastes. Notable among methods currently under development are: (a) batch-operated pot calcination of waste generated from reprocessing stainless steel clad fuel elements (Darex process) and Purex waste, (b) combination rotary kiln and ball mill calcination of aluminum nitrate (TBP-25 and Redox process), and (c) fluidized bed calcination of TBP-25 and Purex wastes. Although a considerable amount of engineering experience has been obtained on the calcination of dissolved salts in a fluidized bed, and the other methods have been the subjects of a great deal of study, none of them have been developed to-the extent which would rule out the desirability of further investigation of other possible methods of calcination.

Luminescence resonance energy transfer-based nucleic acid hybridization assay on cellulose paper with upconverting phosphor as donors.

Science.gov (United States)

Zhou, Feng; Noor, M Omair; Krull, Ulrich J

2014-03-04

A bioassay based on DNA hybridization on cellulose paper is a promising format for gene fragment detection that may be suited for in-field and rapid diagnostic applications. We demonstrate for the first time that luminescence resonance energy transfer (LRET) associated with upconverting phosphors (UCPs) can be used to develop a paper-based DNA hybridization assay with high sensitivity, selectivity and fast response. UCPs with strong green emission were synthesized and subsequently functionalized with streptavidin (UCP-strep). UCP-strep particles were immobilized on cellulose paper, and then biotinylated single-stranded oligonucleotide probes were conjugated onto the UCPs via streptavidin-biotin linkage. The UCPs served as donors that were LRET-paired with Cy3-labeled target DNA. Selective DNA hybridization enabled the proximity required for LRET-sensitized emission from Cy3, which was used as the detection signal. Hybridization was complete within 2 min, and the limit of detection of the method was 34 fmol, which is a significant improvement in comparison to an analogous fluorescence resonance energy transfer (FRET) assay based on quantum dots. The assay exhibited excellent resistance to nonspecific adsorption of noncomplementary short/long DNA and protein. The selectivity of the assay was further evaluated by one base pair mismatched (1BPM) DNA detection, where a maximum signal ratio of 3.1:1 was achieved between fully complementary and 1BPM samples. This work represents a preliminary but significant step for the development of paper-based UCP-LRET nucleic acid hybridization assays, which offer potential for lowering the limit of detection of luminescent hybridization assays due to the negligible background signal associated with optical excitation by near-infrared (NIR) light.

High-performance liquid chromatographic radioenzymatic assay for plasma catecyholamines

International Nuclear Information System (INIS)

Klaniecki, T.S.; Corder, C.N.; McDonald, R.H. Jr.; Feldman, J.A.

1977-01-01

A new assay method for plasma catecholamimes (CA) requiring only 50 μl has been developed, which uses high performance liquid chromatography (HPLC). The norepinephrine (NE), dopamine (D), and epinephrine (E) compounds found in plasma are radioactively o-methylated with S-[methyl- 3 H]-adenosyl-L-methionine ( 3 H-SAM) 3 H-SAM by the reaction of catechol-o-methyl transferase (COMT). The reaction is terminated and a standard mixture of nonradioactive o-methylated analogues of NE, D, and E is added to act as a carrier. Following separation by HPLC, the D,L-normetanephrine (NMN), 3-methoxy-4-hydroxyphenylethyl-amine or 3-methoxytyramine (3-MOT), and metanephrine (MN) radioactive peaks are collected which represent NE, D, and E, respectively. Then MNM and MN are oxidized to vanillin, and 3-MOT is acetylated. The products are subsequently separated by solvent extraction. This is necessary in order to avoid high radioactive blanks and to allow quantitation of the radioactivity by liquid scintillation spectrometry. The mean supine levels of NE, D, and E in normal subjects were respectively 182, 33, and 87 pg/ml of plasma. Similar assays on patients with pheochromocytoma revealed 797, 80, and 470 pg/ml

Development of an ELISA assay for screening inhibitors against divalent metal ion dependent alphavirus capping enzyme.

Science.gov (United States)

Kaur, Ramanjit; Mudgal, Rajat; Narwal, Manju; Tomar, Shailly

2018-06-26

Alphavirus non-structural protein, nsP1 has a distinct molecular mechanism of capping the viral RNAs than the conventional capping mechanism of host. Thus, alphavirus capping enzyme nsP1 is a potential drug target. nsP1 catalyzes the methylation of guanosine triphosphate (GTP) by transferring the methyl group from S-adenosylmethionine (SAM) to a GTP molecule at its N7 position with the help of nsP1 methyltransferase (MTase) followed by guanylylation (GT) reaction which involves the formation of m 7 GMP-nsP1 covalent complex by nsP1 guanylyltransferase (GTase). In subsequent reactions, m 7 GMP moiety is added to the 5' end of the viral ppRNA by nsP1 GTase resulting in the formation of cap0 structure. In the present study, chikungunya virus (CHIKV) nsP1 MTase and GT reactions were confirmed by an indirect non-radioactive colorimetric assay and western blot assay using an antibody specific for the m 7 G cap, respectively. The purified recombinant CHIKV nsP1 has been used for the development of a rapid and sensitive non-radioactive enzyme linked immunosorbent assay (ELISA) to identify the inhibitors of CHIKV nsP1. The MTase reaction is followed by GT reaction and resulted in m 7 GMP-nsP1 covalent complex formation. The developed ELISA nsP1 assay measures this m 7 GMP-nsP1 complex by utilizing anti-m 7 G cap monoclonal antibody. The mutation of a conserved residue Asp63 to Ala revealed its role in nsP1 enzyme reaction. Inductively coupled plasma mass spectroscopy (ICP-MS) was used to determine the presence of magnesium ions (Mg 2+ ) in the purified nsP1 protein. The divalent metal ion selectivity and investigation show preference for Mg 2+ ion by CHIKV nsP1. Additionally, using the developed ELISA nsP1 assay, the inhibitory effects of sinefungin, aurintricarboxylic acid (ATA) and ribavirin were determined and the IC 50 values were estimated to be 2.69 µM, 5.72 µM and 1.18 mM, respectively. Copyright © 2018. Published by Elsevier B.V.

Nondestructive assay of TRU waste using gamma-ray active and passive computed tomography

International Nuclear Information System (INIS)

Roberson, G.P.; Decman, D.; Martz, H.; Keto, E.R.; Johansson, E.M.

1995-01-01

The authors have developed an active and passive computed tomography (A and PCT) scanner for assaying radioactive waste drums. Here they describe the hardware components of their system and the software used for data acquisition, gamma-ray spectroscopy analysis, and image reconstruction. They have measured the performance of the system using ''mock'' waste drums and calibrated radioactive sources. They also describe the results of measurements using this system to assay a real TRU waste drum with relatively low Pu content. The results are compared with X-ray NDE studies of the same TRU waste drum as well as assay results from segmented gamma scanner (SGS) measurements

The influence of non-radiation factors on the kinetics of radioactive iodine metabolism in thyroid

International Nuclear Information System (INIS)

Kalistratova, V.S.; Filatov, V.V.; Shavrina, E.A.

1985-01-01

Estimation of the role non-radiation factors which may influence the dose formation in the most of all irradiated organs is considered very important for the normalization of radioactive isotopes of iodine. The significance of this problem was noted in some recent publications of NCARE. Since a human population which is being irradiated can be affected by the environmental temperature (43-45 deg and 4-6 deg C) and light (complete darkness and darkness for 21 hours) as well as vaccines (dysentery and typhoid) and alcohol (ethanol solutions of 0,02; 0,2; 2,0; and 20,0% concentration) were used as modifying factors. Iodine-125 administrated per os (0,8-1,3 MBq/rat) was used as a model for studying metabolism kinetics of radioactive iodine isotopes. Vaccination of animals results in a change of iodine-125 accumulation level in the thyroid depending on the time interval between an injection of the vaccine and administration of the radionuclide: one day after vaccination the levels of accumulation and the tissue doses formed by radionuclide in the thyroid were decreased by 1,5-2,0 times;14 days after vaccination they were increased by 1,5 times. Accumulation of iodine-125 in other endocrinal organs (pituitary and adrenal glands) was increased. The effect is independent of the type of injected vaccine. A single administration of 2,0-20,0% ethanol caused an increase of iodine-125 content in the thyroid. Chronical exposure to ethanol resulted in a decrease of iodine accumulation in the critical organ by 30% and the tissue doses accumulated by the thyroid, accordingly. Administration 0,02-0,2% ethanol did not affect metabolism kinetics of iodine-125. A single and chronical exposure to higher temperatures (43-45 deg C) decreased accumulation levels and absorbed doses of iodine-125 in the thyroid (by 30-50%) and delayed an elimination of the radionuclide from this organ. Lower temperatures of the environment (4-6 deg C), shorter light day and complete darkness did not affect

The IAEA's activities in the field of radioactive waste management

International Nuclear Information System (INIS)

Semenov, B.A.

1984-01-01

The IAEA has been concerned with radioactive waste management since its inception. Its programme in this area was expanded in the mid 1970s as questions related to the management and disposal of radioactive wastes came into focus in conjunction with the further industrial development of nuclear power. The objectives of the Agency's waste management programme are to assist its Member States in the safe and effective management of wastes by organizing the exchange and dissemination of information, providing guidance and technical assistance and supporting research. The current programme addresses all aspects of the industrial use of nuclear power under the aspects (a) technology of handling and treatment of wastes, (b) underground disposal of wastes, (c) environmental aspects of nuclear energy, including sea disposal of radioactive wastes. Systematic reviews have been made and publications issued concerning the technology of handling, treating, conditioning, and storing various categories of wastes, including liquid and gaseous wastes, wastes from nuclear power plants, spent fuel reprocessing and mining and milling of uranium ores, as well as wastes from decommissioning of nuclear facilities. As waste disposal is the current issue of highest interest, an Agency programme was set up in 1977 to develop a set of guidelines on the safe underground disposal of low-, intermediate- and high-level wastes in shallow ground, rock cavities or deep geological repositories. This programme will continue until 1990. Eleven Safety Series and Technical Documents and Reports have been published under this programme so far, which also addresses safety and other criteria for waste disposal. The environmental part of the waste management programme is concerned with the assessment of radiological and non-radiological consequences of discharges from nuclear facilities, including de minimis concepts in waste disposal and environmental models and data for radionuclide releases

A high-resolution melting (HRM) assay for the differentiation between Israeli field and Neethling vaccine lumpy skin disease viruses.

Science.gov (United States)

Menasherow, Sophia; Erster, Oran; Rubinstein-Giuni, Marisol; Kovtunenko, Anita; Eyngor, Evgeny; Gelman, Boris; Khinich, Evgeny; Stram, Yehuda

2016-06-01

Lumpy skin disease (LSD) is a constant threat to the Middle East including the State of Israel. During vaccination programs it is essential for veterinary services and farmers to be able to distinguish between animals affected by the cattle-borne virulent viruses and vaccinated animals, subsequently affected by the vaccine strain. This study describes an improved high resolution-melting (HRM) test that exploits a 27 base pair (bp) fragment of the LSDV126 extracellular enveloped virion (EEV) gene that is present in field viruses but is absent from the Neethling vaccine strain. This difference leads to ∼0.5 °C melting point change in the HRM assay, when testing the quantitative PCR (qPCR) products generated from the virulent field viruses compared to the attenuated vaccine. By exploiting this difference, it could be shown using the newly developed HRM assay that virus isolated from vaccinated cattle that developed disease symptoms behave similarly to vaccine virus control, indicating that the vaccine virus can induce disease symptoms. This assay is not only in full agreement with the previously published PCR gradient and restriction fragment length polymorphism (RFLP) tests but it is faster with, fewer steps, cheaper and dependable. Copyright © 2016 Elsevier B.V. All rights reserved.

High Level Radioactive Waste Management

International Nuclear Information System (INIS)

1991-01-01

The proceedings of the second annual international conference on High Level Radioactive Waste Management, held on April 28--May 3, 1991, Las Vegas, Nevada, provides information on the current technical issue related to international high level radioactive waste management activities and how they relate to society as a whole. Besides discussing such technical topics as the best form of the waste, the integrity of storage containers, design and construction of a repository, the broader social aspects of these issues are explored in papers on such subjects as conformance to regulations, transportation safety, and public education. By providing this wider perspective of high level radioactive waste management, it becomes apparent that the various disciplines involved in this field are interrelated and that they should work to integrate their waste management activities. Individual records are processed separately for the data bases

Sensing colorimetric approaches based on gold and silver nanoparticles aggregation: Chemical creativity behind the assay. A review

Energy Technology Data Exchange (ETDEWEB)

Vilela, Diana; Gonzalez, Maria Cristina [Departamento de Quimica Analitica e Ingenieria Quimica, Facultad de Quimica, Edificio Polivalente, Universidad de Alcala, Ctra. Madrid-Barcelona km 33,600, 28871 Alcala de Henares, Madrid (Spain); Escarpa, Alberto, E-mail: alberto.escarpa@uah.es [Departamento de Quimica Analitica e Ingenieria Quimica, Facultad de Quimica, Edificio Polivalente, Universidad de Alcala, Ctra. Madrid-Barcelona km 33,600, 28871 Alcala de Henares, Madrid (Spain)

2012-11-02

Highlights: Black-Right-Pointing-Pointer Visual detection based gold and silver nanoparticles aggregation. Black-Right-Pointing-Pointer Functionalized and non-functionalized nanoparticles. Black-Right-Pointing-Pointer High selectivity and sensitivity. Black-Right-Pointing-Pointer No complex instrumentation is required/chemical creativity for analyte detection. - Abstract: Localized surface plasmon resonance (LSPR) is one of the most remarkable features of gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs). Due to these inherent optical properties, colloidal solutions of Au and Ag NPs have high extinction coefficients and different colour in the visible region of the spectrum when they are well-spaced in comparison with when they are aggregated. Therefore, a well-designed chemical interaction between the analyte and NPs surroundings leads to a change of colour (red to blue for Au NPs and yellow to brown for Ag NPs from well-spaced to aggregated ones, respectively) allowing the visual detection of the target analyte. These approaches have exhibited an excellent analytical performance with high sensitivities due to the strong LSPR and excellent selectivity strategically driven by the interaction analyte-NPs surroundings involving mainly electrostatic and hydrogen bond interactions as well as donor-acceptor chemical reactions, among others. In addition, this kind of colorimetric assays has received considerable attention in the analytical field because of their simplicity and low cost since they do not require any expensive or complex instrumentation. As a consequence of this, detection of molecules with a high significance in the bio-medical, clinical, food safety and environmental fields including DNA, proteins and a wide spectrum of organic molecules as well as inorganic ions have been impressively reported in the most relevant literature using these assays. This timely review offers a rational vision of the main achievements yielded in the relevant

Sensing colorimetric approaches based on gold and silver nanoparticles aggregation: Chemical creativity behind the assay. A review

International Nuclear Information System (INIS)

Vilela, Diana; González, María Cristina; Escarpa, Alberto

2012-01-01

Highlights: ► Visual detection based gold and silver nanoparticles aggregation. ► Functionalized and non-functionalized nanoparticles. ► High selectivity and sensitivity. ► No complex instrumentation is required/chemical creativity for analyte detection. - Abstract: Localized surface plasmon resonance (LSPR) is one of the most remarkable features of gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs). Due to these inherent optical properties, colloidal solutions of Au and Ag NPs have high extinction coefficients and different colour in the visible region of the spectrum when they are well-spaced in comparison with when they are aggregated. Therefore, a well-designed chemical interaction between the analyte and NPs surroundings leads to a change of colour (red to blue for Au NPs and yellow to brown for Ag NPs from well-spaced to aggregated ones, respectively) allowing the visual detection of the target analyte. These approaches have exhibited an excellent analytical performance with high sensitivities due to the strong LSPR and excellent selectivity strategically driven by the interaction analyte-NPs surroundings involving mainly electrostatic and hydrogen bond interactions as well as donor–acceptor chemical reactions, among others. In addition, this kind of colorimetric assays has received considerable attention in the analytical field because of their simplicity and low cost since they do not require any expensive or complex instrumentation. As a consequence of this, detection of molecules with a high significance in the bio-medical, clinical, food safety and environmental fields including DNA, proteins and a wide spectrum of organic molecules as well as inorganic ions have been impressively reported in the most relevant literature using these assays. This timely review offers a rational vision of the main achievements yielded in the relevant literature according to this exciting and creative analytical field.

Preclinical detection of porcine circovirus type 2 infection using an ultrasensitive nanoparticle DNA probe-based PCR assay.

Directory of Open Access Journals (Sweden)

Yong Huang

Full Text Available Porcine circovirus type 2 (PCV2 has emerged as one of the most important pathogens affecting swine production globally. Preclinical identification of PCV2 is very important for effective prophylaxis of PCV2-associated diseases. In this study, we developed an ultrasensitive nanoparticle DNA probe-based PCR assay (UNDP-PCR for PCV2 detection. Magnetic microparticles coated with PCV2 specific DNA probes were used to enrich PCV2 DNA from samples, then gold nanoparticles coated with PCV2 specific oligonucleotides were added to form a sandwich nucleic acid-complex. After the complex was formed, the oligonucleotides were released and characterized by PCR. This assay exhibited about 500-fold more sensitive than conventional PCR, with a detection limit of 2 copies of purified PCV2 genomic DNA and 10 viral copies of PCV2 in serum. The assay has a wide detection range for all of PCV2 genotypes with reliable reproducibility. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1, porcine parvovirus, porcine pseudorabies virus, porcine reproductive and respiratory syndrome virus and classical swine fever virus. The positive detection rate of PCV2 specific UNDP-PCR in 40 preclinical field samples was 27.5%, which appeared greater than that by conventional and real-time PCR and appeared application potency in evaluation of the viral loads levels of preclinical infection samples. The UNDP-PCR assay reported here can reliably rule out false negative results from antibody-based assays, provide a nucleic acid extraction free, specific, ultrasensitive, economic and rapid diagnosis method for preclinical PCV2 infection in field, which may help prevent large-scale outbreaks.

The Application and Regulation of Non-Medical radioactive Substances in Taiwan, China

International Nuclear Information System (INIS)

Chang, Chihchien; Chou, Keiden; Wang, Songfeng

1998-01-01

Based on the Atomic Energy Law of Taiwan and regulations regarding radiation protection, an operating system has been established for the approval and regulation of import (production), installation, licensing, safety inspection, record keeping, storage, transfer, transportation and abandonment of nonmedical radioactive materials and equipment capable of producing ionizing radiation. In order to ensure that all equipment capable of producing ionizing radiation can meet the respective standard of radiation protection in accordance with the ALARA principle, nonmedical equipment capable of producing ionizing radiation is divided into six categories depending on its inherent shielding ability, operation limit, characteristics of the radiation and the required degree of surveillance for achieving the purpose of radiation protection. The six categories are: 1. Protective equipment, 2. Immobile closed equipment, 3. Automatic operating equipment, 4. Mobile equipment, 5. Unsealed radioactive substances, 6. Consumer products and other radioactive sources with different properties. Each category has its specific requirements in radiation protection. (author)

Development of a solid-phase assay for measurement of proteolytic enzyme activity

International Nuclear Information System (INIS)

Varani, J.; Johnson, K.; Kaplan, J.

1980-01-01

A solid-phase, plate assay was developed for the measurement of proteolytic enzyme activity. In this assay procedure, radiolabeled substrates were dried onto the surface of microtiter wells. Following drying, the wells were washed two times with saline to remove the nonadherent substrate. When proteolytic enzymes were added to the wells, protein hydrolysis occurred, releasing radioactivity into the supernatant fluid. The amount of protein hydrolysis that occurred was reflected by the amount of radioactivity in the supernatant fluid. When 125 I-hemoglobin was used as the substrate, it was as susceptible to hydrolysis by trypsin in the solid-phase assay as it was in solution in a standard assay procedure. Protease activity from a variety of sources (including from viable cells as well as from extracellular sources) were also able to hydrolyze the hemoglobin on the plate. 125 I-Labeled serum albumen, fibrinogen, and rat pulmonary basement membrane were also susceptible to hydrolysis by trypsin in the solid phase. When [ 14 C]elastin was dried onto the plate, it behaved in a similar manner to elastin in solution. It was resistant to hydrolysis by nonspecific proteases such as trypsin and chymotrypsin but was highly susceptible to hydrolysis by elastase. The solid-phase plate assay has several features which recommended it for routine use. It is as sensitive as standard tube assays (and much more sensitive than routinely used colormetric assays). It is quick and convenient; there are no precipitation, centrifugation, or filtration steps. In addition, very small volumes of radioactive wastes are generated. Another advantage of the solid-phase plate assay is the resistance of the dried substrates to spontaneous breakdown and to microbial contamination. Finally, this assay is suitable for use with viable cells as well as for extracellular proteases

Radioactive Waste Repositories Administration - SURAO

International Nuclear Information System (INIS)

Kucerka, M.

1998-01-01

The Atomic Act specifies, among other things, responsibilities of the government in the field of safe disposal of radioactive wastes. To satisfy this responsibility, the Ministry of Industry and Trade has established the Radioactive Waste Repositories Administration (SURAO). SURAO's major responsibilities include: (a) the preparation, construction, commissioning, operation, and decommissioning of radioactive waste repositories and the monitoring of their environmental impacts; (b) radioactive waste management; (c) spent or irradiated nuclear fuel processing into a form suitable for storage/disposal or reuse; (d) record-keeping of received radioactive wastes and their producers; (e) administration of fund transfers as stipulated by the Atomic Act, Article 27; (f) development of proposals for specification of fees to be paid to the Nuclear Account; (g) responsibility for and coordination of research and development in the field of radioactive waste handling and management; (h) supervision of licensees' margin earmarked for the decommissioning of their facilities; (i) providing services in radioactive waste handling and management; (j) handling and management of radioactive wastes that have been transferred to the Czech Republic from abroad and cannot be sent back; (k) interim administration of radioactive wastes that have become state property. The Statute of the Administration is reproduced in full. (P.A.)

Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?

International Nuclear Information System (INIS)

Klobucar, Goeran I.V.; Stambuk, Anamaria; Srut, Maja; Husnjak, Ivana; Merkas, Martina; Traven, Luka; Cvetkovic, Zelimira

2011-01-01

There is a growing interest for the application of biomakers to field-collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field-collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm the employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys. - Research highlights: → Native A. caliginosa has shown significant biological effect measured by the Comet and NRRT assays. → The Comet assay on A. caliginosa and E. fetida has shown to be of similar sensitivity as the NRRT assay. → A. caliginosa is a suitable species for the in situ soil toxicity and genotoxicity field surveys. - Native populations of endogeic earthworm Aporrectodea caliginosa can be successfully applied in the genotoxicity field surveys using Comet assay.

Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?

Energy Technology Data Exchange (ETDEWEB)

Klobucar, Goeran I.V., E-mail: gklobuca@zg.biol.pmf.hr [Department of Zoology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb (Croatia); Stambuk, Anamaria; Srut, Maja [Department of Zoology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb (Croatia); Husnjak, Ivana [Ministry of Environmental Protection, Physical Planning and Construction, Ulica Republike Austrije 14, Zagreb (Croatia); Merkas, Martina [Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 12, 10000 Zagreb (Croatia); Traven, Luka [Department of Environmental Medicine, Medical Faculty, University of Rijeka, Brace Branchetta 20a, 51000 Rijeka (Croatia); Teaching Institute of Public Health of the Primorsko-goranska County, Kresimirova 52a, 51000 Rijeka (Croatia); Cvetkovic, Zelimira [Department of Ecology, Institute of Public Health, Mirogojska c. 16, 10000 Zagreb (Croatia)

2011-04-15

There is a growing interest for the application of biomakers to field-collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field-collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm the employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys. - Research highlights: > Native A. caliginosa has shown significant biological effect measured by the Comet and NRRT assays. > The Comet assay on A. caliginosa and E. fetida has shown to be of similar sensitivity as the NRRT assay. > A. caliginosa is a suitable species for the in situ soil toxicity and genotoxicity field surveys. - Native populations of endogeic earthworm Aporrectodea caliginosa can be successfully applied in the genotoxicity field surveys using Comet assay.

Polarized secondary radioactive beams

International Nuclear Information System (INIS)

Zaika, N.I.

1992-01-01

Three methods of polarized radioactive nuclei beam production: a) a method nuclear interaction of the non-polarized or polarized charged projectiles with target nuclei; b) a method of polarization of stopped reaction radioactive products in a special polarized ion source with than following acceleration; c) a polarization of radioactive nuclei circulating in a storage ring are considered. Possible life times of the radioactive ions for these methods are determined. General schemes of the polarization method realizations and depolarization problems are discussed

Radioactive waste from non-licensed activities - identification of waste, compilation of principles and guidance, and proposed system for final management; Radioaktivt avfall fraan icke tillstaandsbunden verksamhet (RAKET) - identifiering av aktuellt avfall, sammanstaellning av relevanta regler och principer, foerslag paa system foer omhaendertagande

Energy Technology Data Exchange (ETDEWEB)

Jones, C.; Pers, K. [Kemakta Konsult AB, Stockholm (Sweden)

2001-07-01

Presently national guidelines for the handling of radioactive waste from non-licensed activities are lacking in Sweden. Results and information presented in this report are intended to form a part of the basis for decisions on further work within the Swedish Radiation Protection Institute on regulations or other guidelines on final management and final disposal of this type of waste. An inventory of radioactive waste from non-licensed activities is presented in the report. In addition, existing rules and principles used in Sweden - and internationally - on the handling of radioactive and toxic waste and non-radioactive material are summarized. Based on these rules and principles a system is suggested for the final management of radioactive material from non-licensed activities. A model is shown for the estimation of dose as a consequence of leaching of radio-nuclides from different deposits. The model is applied on different types of waste, e.g. peat ashes, light concrete and low-level waste from a nuclear installation.

Neutral atom traps of radioactives

International Nuclear Information System (INIS)

Behr, J.A.

2003-01-01

Neutral atoms trapped with modern laser cooling techniques offer the promise of improving several broad classes of experiments with radioactive isotopes. In nuclear β decay, neutrino spectroscopy from beta-recoil coincidences, along with highly polarized samples, enable experiments to search for non-Standard Model interactions, test whether parity symmetry is maximally violated, and search for new sources of time reversal violation. Ongoing efforts at TRIUMF, Los Alamos and Berkeley will be highlighted. The traps also offer bright sources for Doppler-free spectroscopy, particularly in high-Z atoms where precision measurements could measure the strength of weak neutral nucleon-nucleon and electron-nucleon interactions. Physics with francium atoms has been vigorously pursued at Stony Brook. Several facilities plan work with radioactive atom traps; concrete plans and efforts at KVI Groningen and Legnaro will be among those summarized. Contributions to the multidisciplinary field of trace analysis will be left up to other presenters

Neutral atom traps of radioactives

CERN Document Server

Behr, J A

2003-01-01

Neutral atoms trapped with modern laser cooling techniques offer the promise of improving several broad classes of experiments with radioactive isotopes. In nuclear beta decay, neutrino spectroscopy from beta-recoil coincidences, along with highly polarized samples, enable experiments to search for non-Standard Model interactions, test whether parity symmetry is maximally violated, and search for new sources of time reversal violation. Ongoing efforts at TRIUMF, Los Alamos and Berkeley will be highlighted. The traps also offer bright sources for Doppler-free spectroscopy, particularly in high-Z atoms where precision measurements could measure the strength of weak neutral nucleon-nucleon and electron-nucleon interactions. Physics with francium atoms has been vigorously pursued at Stony Brook. Several facilities plan work with radioactive atom traps; concrete plans and efforts at KVI Groningen and Legnaro will be among those summarized. Contributions to the multidisciplinary field of trace analysis will be left...

The influence of organic materials on the near field of an intermediate level waste radioactive waste repository

International Nuclear Information System (INIS)

Wilkins, J.D.

1988-02-01

The influence of organic materials, which are present in some intermediate level wastes, on the chemistry of the near field of a radioactive waste repository is discussed. Particular attention is given to the possible formation of water soluble complexing agents formed as a result of the radiation field and chemical conditions. The present state of the research is reviewed. (author)

Incineration of European non-nuclear radioactive waste in the USA

International Nuclear Information System (INIS)

Moloney, B. P.; Ferguson, D.; Stephenson, B.

2013-01-01

Incineration of dry low level radioactive waste from nuclear stations is a well established process achieving high volume reduction factors to minimise disposal costs and to stabilise residues for disposal. Incineration has also been applied successfully in many European Union member countries to wastes arising from use of radionuclides in medicine, nonnuclear research and industry. However, some nations have preferred to accumulate wastes over many years in decay stores to reduce the radioactive burden at point of processing. After decay and sorting the waste, they then require a safe, industrial scale and affordable processing solution for the large volumes accumulated. This paper reports the regulatory, logistical and technical issues encountered in a programme delivered for Eckert and Ziegler Nuclitec to incinerate safely 100 te of waste collected originally from German research, hospital and industrial centres, applying for the first time a 'burn and return' process model for European waste in the US. The EnergySolutions incinerators at Bear Creek, Oak Ridge, Tennessee, USA routinely incinerate waste arising from the non-nuclear user community. To address the requirement from Germany, EnergySolutions had to run a dedicated campaign to reduce cross-contamination with non-German radionuclides to the practical minimum. The waste itself had to be sampled in a carefully controlled programme to ensure the exacting standards of Bear Creek's license and US emissions laws were maintained. Innovation was required in packaging of the waste to minimise transportation costs, including sea freight. The incineration was inspected on behalf of the German regulator (the BfS) to ensure suitability for return to Germany and disposal. This first 'burn and return' programme has safely completed the incineration phase in February and the arising ash will be returned to Germany presently. The paper reports the main findings and lessons learned on this first

Ambient radioactivity levels and radiation doses. Annual report 2009

International Nuclear Information System (INIS)

Bernhard-Stroel, Claudia; Hachenburger, Claudia; Trugenberger-Schnabel, Angela; Peter, Josef

2010-12-01

The annual report on environmental radioactivity and radiation exposure 2009 consists of two parts. Part A: General information: natural environmental radioactivity, artificial radioactivity in the environment, occupational radiation exposure, radiation exposures from medical applications, the handling of radioactive materials and sources of ionizing radiation, non-ionizing radiation. Part B includes current data and their evaluation for natural environmental radioactivity, artificial radioactivity in the environment, occupational radiation exposure, radiation exposures from medical applications, the handling of radioactive materials and sources of ionizing radiation, non-ionizing radiation.

Radioactive probes as diagnostic tools for rice tungro viruses

International Nuclear Information System (INIS)

Azzam, O.; Arboleda, M.; Reyes. J. de los

1996-01-01

Rice tungro bacilliform (RTBV) and rice tungro spherical viruses (RTSV) are the two viral components responsible for rice tungro disease which has seriously affected the irrigated rice ecosystem in Southeast Asia for the last 30 years. RTBV has an 8 Kb double-stranded DNA circular genome, and it is primarily responsible for induction of symptoms in infected plants. RTSV has a 12 kb single-stranded RNA genome. It does not induce any apparent symptoms in the infected plant, and it is transmitted by greenleafhopper. RTBV depends upon RTSV for its own transmission. The two viruses are limited to the vascular tissue of the rice plant and are present at a low titer. Most of the detection methods used for the identification of these viruses have relied on the virus protein properties and therefore, early detection of the virus activity was not possible. We were interested in evaluating tissue printing, dot blot, and southern techniques for early detection of virus nucleic acids in rice plant using radioactive and non radioactive probes. 32 P-labeled T7 or SP6 RNA polymerase transcripts complementary to the RTBV genome and RTSV coat protein genes were used as probes of the positive stand of both viruses. For nonradioactive probes, RTBV DNA genome was labeled using the ECL detection kit (Amersham). Preliminary results show that viral nucleic acids of RTBV and RTSV could be detected using both labelling systems. Non radioactive probes were comparable in their sensitivity to the radioactive probes. Less than 100 pg of viral DNA was detected in the dot-blot assays. More data will be presented to compare the efficiency and reliability of these two techniques in detecting early virus activity in the rice plant. (author)

Developing a yeast-based assay protocol to monitor total ...

African Journals Online (AJOL)

A yeast-based assay protocol developed for detecting oestrogenic activity in activated sludge (AS) supernatant is described. The protocol used Saccharomyces cerevisiae construct RMY/ER-ERE with human oestrogen receptor (ERα) and lacZ reporter genes, and was developed by modifying existing assays for use with AS ...

Radioactivity; La radioactivite

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-07-01

This pedagogical document presents the origin, effects and uses of radioactivity: where does radioactivity comes from, effects on the body, measurement, protection against radiations, uses in the medical field, in the electric power industry, in the food (ionization, radio-mutagenesis, irradiations) and other industries (radiography, gauges, detectors, irradiations, tracers), and in research activities (dating, preservation of cultural objects). The document ends with some examples of irradiation levels (examples of natural radioactivity, distribution of the various sources of exposure in France). (J.S.)

Radioactive waste management for a radiologically contaminated hospitalized patient

International Nuclear Information System (INIS)

Pina Jomir, G.; Michel, X.; Lecompte, Y.; Chianea, N.; Cazoulat, A.

2015-01-01

Radioactive waste management in the post-accidental phase following caring for a radiologically contaminated patient in a hospital decontamination facility must be anticipated at a local level to be truly efficient, as the volume of waste could be substantial. This management must comply with the principles set out for radioactive as well as medical waste. The first step involves identification of radiologically contaminated waste based on radioactivity measurement for volume reduction. Then, the management depends on the longest radioactive half-life of contaminative radionuclides. For a half-life inferior to 100 days, wastes are stored for their radioactivity to decay for at least 10 periods before disposal like conventional medical waste. Long-lived radioactive waste management implies treatment of liquid waste and special handling for sorting and packaging before final elimination at the French National Agency for Radioactive Waste Management (ANDRA). Following this, highly specialized waste management skills, financial responsibility issues and detention of non-medical radioactive sources are questions raised by hospital radioactive waste management in the post-accidental phase. (authors)

A development of radioactive material tracking and location control system

International Nuclear Information System (INIS)

Joo, Gwang Tae; Jung Seung Yong; Song, Jung Ho

2005-01-01

As the whole industry fields such as construction, chemistry, machine, medicine including nuclear-related field have extended the range of radioactive material uses, it is tendency that domestic uses of radioactive material have been increased in quantity and number. In addition, as the transportation, transfer and use of radioactive material have been frequent, its loss, robbery, and carelessness of handling may cause not only employees in charge but the public to worry about damage of explosion and put an obstacle to increase trust in nuclear-related industry. At present, though the transportation, use and storage of radioactive material conform to the institution and standard of the atomic energy law, if we tracking radioactive material in real time, we can take immediate actions to prevent its loss, robbery. As our research institute developed a terminal that control location and tracking real time location for gamma-ray projector used in transporting, transferring, and using nondestructive test, we take a good look at utilities by using GPS-Cell ID bases location control

Ecotoxicological assays of Diethyltoluamide and Lemongrass Essencial Oil in irradiated and non-irradiated aquatic organisms

International Nuclear Information System (INIS)

Gimiliani, Giovana T.; Rogero, Sizue O.; Martini, Gisela A.; Rogero, Jose R.

2015-01-01

Aquatic invertebrates can be potentially exposed to nonradioactive contaminants in conjunction with ionizing radiation, especially in highly industrialized areas surrounding nuclear facilities, where radionuclides can accidentally be discharged in the aquatic environment containing stable chemicals. The aquatic organisms have continually been exposed to chemical contaminants like personal care products (PCPs) which have been found in various environmental matrices and may cause adverse effects to aquatic life and human health as radioactive products. In this study was used C. silvestrii as bioindicator organism in chronic ecotoxicity assays with lemongrass essencial oil (LEO) and Diethyltoluamide (DEET), both are insect repellent. In addition to exposition of the compounds, the organisms were irradiated with gamma rays from Co-60 source. Thus, the aim of this study was to evaluate the possible synergistic effect of gamma radiation and mosquito repellent products in the reproduction of Ceriodaphnia silvestrii utilizing standardized ecotoxicological tests. The C. silvestrii inhibition concentration (IC25; 7 days) result after DEET exposition was 16.4 ± 1.4 mg L -1 and for LEO was 3.1 ± 1.4 mg L -1 . In the irradiated (25 Gy) C. silvestrii exposed to DEET and LEO, the concentration that inhibited reproduction was 16.1 ± 0.9 mg L -1 and 2.4 ± 0.3 mg L -1 respectively. The results showed that the reproduction of irradiated C. silvestrii was not significantly affected when compared with non-irradiated organisms when exposed to DEET or LEO. (author)

Ecotoxicological assays of Diethyltoluamide and Lemongrass Essencial Oil in irradiated and non-irradiated aquatic organisms

Energy Technology Data Exchange (ETDEWEB)

Gimiliani, Giovana T.; Rogero, Sizue O.; Martini, Gisela A.; Rogero, Jose R., E-mail: sorogero@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

2015-07-01

Aquatic invertebrates can be potentially exposed to nonradioactive contaminants in conjunction with ionizing radiation, especially in highly industrialized areas surrounding nuclear facilities, where radionuclides can accidentally be discharged in the aquatic environment containing stable chemicals. The aquatic organisms have continually been exposed to chemical contaminants like personal care products (PCPs) which have been found in various environmental matrices and may cause adverse effects to aquatic life and human health as radioactive products. In this study was used C. silvestrii as bioindicator organism in chronic ecotoxicity assays with lemongrass essencial oil (LEO) and Diethyltoluamide (DEET), both are insect repellent. In addition to exposition of the compounds, the organisms were irradiated with gamma rays from Co-60 source. Thus, the aim of this study was to evaluate the possible synergistic effect of gamma radiation and mosquito repellent products in the reproduction of Ceriodaphnia silvestrii utilizing standardized ecotoxicological tests. The C. silvestrii inhibition concentration (IC25; 7 days) result after DEET exposition was 16.4 ± 1.4 mg L{sup -1} and for LEO was 3.1 ± 1.4 mg L{sup -1}. In the irradiated (25 Gy) C. silvestrii exposed to DEET and LEO, the concentration that inhibited reproduction was 16.1 ± 0.9 mg L{sup -1} and 2.4 ± 0.3 mg L{sup -1} respectively. The results showed that the reproduction of irradiated C. silvestrii was not significantly affected when compared with non-irradiated organisms when exposed to DEET or LEO. (author)

Screening calculations for radioactive waste releases from non-nuclear facilities

International Nuclear Information System (INIS)

Xu, Shulan; Soederman, Ann-Louis

2009-02-01

A series of screening calculations have been performed to assess the potential radiological consequences of discharges of radioactive substances to the environment arising from waste from non-nuclear practices. Solid waste, as well as liquids that are not poured to the sewer, are incinerated and ashes from incineration and sludge from waste water treatment plants are disposed or reused at municipal disposal facilities. Airborne discharges refer to releases from an incineration facility and liquid discharges refer both to releases from hospitals and laboratories to the sewage system, as well as leakage from waste disposal facilities. The external exposure of workers is estimated both in the waste water treatment plant and at the disposal facility. The calculations follow the philosophy of the IAEA's safety guidance starting with a simple assessment based on very conservative assumptions which may be iteratively refined using progressively more complex models, with more realistic assumptions, as necessary. In the assessments of these types of disposal, with cautious assumptions, carried out in this report we conclude that the radiological impacts on representative individuals in the public are negligible in that they are small with respect to the target dose of 10 μSv/a. A Gaussian plume model was used to estimate the doses from airborne discharges from the incinerator and left a significant safety margin in the results considering the conservative assumptions in the calculations. For the sewage plant workers the realistic approach included a reduction in working hours and the shorter exposure time resulted in maximum doses around 10 μSv/a. The calculations for the waste disposal facility show that the doses are higher or in the range of the target dose. The excess for public exposure is mainly caused by H-3 and C-14. The assumption used in the calculation is that all of the radioactive substances sent to the incineration facility and waste water treatment plant

Screening calculations for radioactive waste releases from non-nuclear facilities

Energy Technology Data Exchange (ETDEWEB)

Shulan Xu; Soederman, Ann-Louis

2009-02-15

A series of screening calculations have been performed to assess the potential radiological consequences of discharges of radioactive substances to the environment arising from waste from non-nuclear practices. Solid waste, as well as liquids that are not poured to the sewer, are incinerated and ashes from incineration and sludge from waste water treatment plants are disposed or reused at municipal disposal facilities. Airborne discharges refer to releases from an incineration facility and liquid discharges refer both to releases from hospitals and laboratories to the sewage system, as well as leakage from waste disposal facilities. The external exposure of workers is estimated both in the waste water treatment plant and at the disposal facility. The calculations follow the philosophy of the IAEA's safety guidance starting with a simple assessment based on very conservative assumptions which may be iteratively refined using progressively more complex models, with more realistic assumptions, as necessary. In the assessments of these types of disposal, with cautious assumptions, carried out in this report we conclude that the radiological impacts on representative individuals in the public are negligible in that they are small with respect to the target dose of 10 muSv/a. A Gaussian plume model was used to estimate the doses from airborne discharges from the incinerator and left a significant safety margin in the results considering the conservative assumptions in the calculations. For the sewage plant workers the realistic approach included a reduction in working hours and the shorter exposure time resulted in maximum doses around 10 muSv/a. The calculations for the waste disposal facility show that the doses are higher or in the range of the target dose. The excess for public exposure is mainly caused by H-3 and C-14. The assumption used in the calculation is that all of the radioactive substances sent to the incineration facility and waste water treatment

A compact HV supply for field/PC based nuclear instrumentation

International Nuclear Information System (INIS)

Manna, A.; Nikhare, D.M.; Madhavi, V.; Bayala, A.K.; Mukhopadhyay, P.K.; Kataria, S.K.

2001-01-01

In the recent years, most of the nuclear instruments that were earlier based on NIM Bin standards, are becoming available as PC Add-on cards. This trend is due to the decreasing prices of desktop personal computers and the necessity for automation in radioactivity measurements. This paper describes the design and development of a HV supply module and its PC Add-on card version for field portable/ PC based nuclear instrumentation. The HV supply though being very compact in size meets all the stringent specifications required for detector biasing applications and it has been tested for use with NaI, BF 3 . (author)

Non-Invasive Imaging Method of Microwave Near Field Based on Solid State Quantum Sensing

OpenAIRE

Yang, Bo; Du, Guanxiang; Dong, Yue; Liu, Guoquan; Hu, Zhenzhong; Wang, Yongjin

2018-01-01

In this paper, we propose a non-invasive imaging method of microwave near field using a diamond containing nitrogen-vacancy centers. We applied synchronous pulsed sequence combined with charge coupled device camera to measure the amplitude of the microwave magnetic field. A full reconstruction formulation of the local field vector, including the amplitude and phase, is developed by measuring both left and right circular polarizations along the four nitrogen-vacancy axes. Compared to the raste...

Comet assay as a procedure for detecting possible genotoxicity induced by non-ionizing radiation

Directory of Open Access Journals (Sweden)

Zsuzsanna Nemeth

2015-05-01

In our laboratory we use comet assay for testing genotoxicity of non-ionizing radiation for more than ten years. In the experiments we use whole blood samples (human or dog, cell lines (e.g. H295R cell line or 3 dimensional in vitro skin tissue (epidermis models. In our protocol a slightly modified alkaline Comet assay method of Singh et al. (1988 is used. On our poster there will be presented a brief summary of our experiments with exposure to different types of radiation (ELF, RF, and intermediate frequency. In our protocols the non-ionizing radiation was often combined with ionizing radiation to see whether the non-ionizing radiation can influence the repair of the DNA damage induced by ionizing radiation. For the evaluation of the slides mainly Komet 4.0 image analysis system software (Kinetic Imaging, Liverpool, UK was used, but as we got familiarized with other methods for slide evaluation like grading the comets by visual scoring into 5 categories or the CaspLab software, the comparison of these three methods will be also presented.

Non-Abelian gauge fields

Science.gov (United States)

Gerbier, Fabrice; Goldman, Nathan; Lewenstein, Maciej; Sengstock, Klaus

2013-07-01

interesting and related effect, which arises from the interplay between strong magnetic field and lattice potentials, is the famous Hofstadter butterfly: the energy spectrum of a single particle moving on a lattice and subjected to a strong magnetic field displays a beautiful fractal structure as a function of the magnetic flux penetrating each elementary plaquette of the lattice. When the effects of interparticle interactions become dominant, two-dimensional gases of electrons exhibit even more exotic behaviour leading to the fractional quantum Hall effect. In certain conditions such a strongly interacting electron gas may form a highly correlated state of matter, the prototypical example being the celebrated Laughlin quantum liquid. Even more fascinating is the behaviour of bulk excitations (quasi-hole and quasi-particles): they are neither fermionic nor bosonic, but rather behave as anyons with fractional statistics intermediate between the two. Moreover, for some specific filling factors (ratio between the electronic density and the flux density), these anyons are proven to have an internal structure (several components) and non-Abelian braiding properties. Many of the above statements concern theoretical predictions—they have never been observed in condensed matter systems. For instance, the fractional values of the Hall conductance is seen as a direct consequence of the fractional statistics, but to date direct observation of anyons has not been possible in two-dimensional semiconductors. Realizing these predictions in experiments with atoms, ions, photons etc, which potentially allow the experimentalist to perform measurements complementary to those made in condensed matter systems, is thus highly desirable! Non-Abelian gauge fields couple the motional states of the particles to their internal degrees of freedom (such as hyperfine states for atoms or ions, electronic spins for electrons, etc). In this sense external non-Abelian fields extend the concept of spin

Validation of Non-Invasive Waste Assay System (Gamma Box Counter) Performance at AECL Whiteshell Laboratories - 13136

International Nuclear Information System (INIS)

Attas, E.M.; Bialas, E.; Rhodes, M.J.

2013-01-01

Low-level radioactive waste (LLW) in solid form, resulting from decommissioning and operations activities at AECL's Whiteshell Laboratories (WL), is packaged in B-25 and B-1000 standard waste containers and characterized before it is shipped to an on-site interim storage facility, pending AECL decisions on long term management of its LLW. Assay of the waste packages before shipment contributes to an inventory of the interim storage facility and provides data to support acceptance at a future repository. A key characterization step is a gamma spectrometric measurement carried out under standard conditions using an automated, multi-detector Waste Assay System (WAS), purchased from Antech Corporation. A combination of ORTEC gamma acquisition software and custom software is used in this system to incorporate multiple measurements from two collimated high-resolution detectors. The software corrects the intensities of the gamma spectral lines for geometry and attenuation, and generates a table of calculated activities or limits of detection for a user-defined list of radioisotopes that may potentially be present. Validation of WAS performance was a prerequisite to routine operation. Documentation of the validation process provides assurance of the quality of the results produced, which may be needed one or two decades after they were generated. Aspects of the validation included setting up a quality control routine, measurements of standard point sources in reproducible positions, study of the gamma background, optimization of user-selectable software parameters, investigation of the effect of non-uniform distribution of materials and radionuclides, and comparison of results with measurements made using other gamma detector systems designed to assay bulk materials. The following key components of the validation process have been established. A daily quality control routine has been instituted, to verify stability of the gamma detector operation and the background levels

Mixed radioactive and chemotoxic wastes (RMW)

International Nuclear Information System (INIS)

Dejonghe, I.P.

1991-01-01

During the first decades of development of nuclear energy, organizations involved in the management of nuclear wastes had their attention focused essentially on radioactive components. The impression may have prevailed that, considering the severe restrictions on radioactive materials, the protection measured applied for radioactive components of wastes would be more than adequate to cope with potential hazards from non radioactive components associated with radioactive wastes. More recently it was acknowledged that such interpretation is not necessarily justified in all cases since certain radioactive wastes also contain non-negligible amounts of heavy metals or hazardous organic components which, either, do not decay, or are subject to completely different decay (decomposition) mechanisms. The main purposes of the present study are to analyze whether mixed radioactive wastes are likely to occur in Europe and in what form, whether one needs a basis for integration for evaluating various forms of toxicity and by which practical interventions possible problems can be avoided or at least reduced. (au)

A tissue biopsy-based epigenetic multiplex PCR assay for prostate cancer detection

Directory of Open Access Journals (Sweden)

Van Neste Leander

2012-06-01

Full Text Available Abstract Background PSA-directed prostate cancer screening leads to a high rate of false positive identifications and an unnecessary biopsy burden. Epigenetic biomarkers have proven useful, exhibiting frequent and abundant inactivation of tumor suppressor genes through such mechanisms. An epigenetic, multiplex PCR test for prostate cancer diagnosis could provide physicians with better tools to help their patients. Biomarkers like GSTP1, APC and RASSF1 have demonstrated involvement with prostate cancer, with the latter two genes playing prominent roles in the field effect. The epigenetic states of these genes can be used to assess the likelihood of cancer presence or absence. Results An initial test cohort of 30 prostate cancer-positive samples and 12 cancer-negative samples was used as basis for the development and optimization of an epigenetic multiplex assay based on the GSTP1, APC and RASSF1 genes, using methylation specific PCR (MSP. The effect of prostate needle core biopsy sample volume and age of formalin-fixed paraffin-embedded (FFPE samples was evaluated on an independent follow-up cohort of 51 cancer-positive patients. Multiplexing affects copy number calculations in a consistent way per assay. Methylation ratios are therefore altered compared to the respective singleplex assays, but the correlation with patient outcome remains equivalent. In addition, tissue-biopsy samples as small as 20 μm can be used to detect methylation in a reliable manner. The age of FFPE-samples does have a negative impact on DNA quality and quantity. Conclusions The developed multiplex assay appears functionally similar to individual singleplex assays, with the benefit of lower tissue requirements, lower cost and decreased signal variation. This assay can be applied to small biopsy specimens, down to 20 microns, widening clinical applicability. Increasing the sample volume can compensate the loss of DNA quality and quantity in older samples.

A novel quantitative kinase assay using bacterial surface display and flow cytometry.

Directory of Open Access Journals (Sweden)

Sónia Troeira Henriques

Full Text Available The inhibition of tyrosine kinases is a successful approach for the treatment of cancers and the discovery of kinase inhibitor drugs is the focus of numerous academic and pharmaceutical laboratories. With this goal in mind, several strategies have been developed to measure kinase activity and to screen novel tyrosine kinase inhibitors. Nevertheless, a general non-radioactive and inexpensive approach, easy to implement and adapt to a range of applications, is still missing. Herein, using Bcr-Abl tyrosine kinase, an oncogenic target and a model protein for cancer studies, we describe a novel cost-effective high-throughput screening kinase assay. In this approach, named the BacKin assay, substrates displayed on a Bacterial cell surface are incubated with Kinase and their phosphorylation is examined and quantified by flow cytometry. This approach has several advantages over existing approaches, as using bacteria (i.e. Escherichia coli to display peptide substrates provides a self renewing solid support that does not require laborious chemical strategies. Here we show that the BacKin approach can be used for kinetic and mechanistic studies, as well as a platform to characterize and identify small-molecule or peptide-based kinase inhibitors with potential applications in drug development.

The technologically-reinforced natural radioactivity

International Nuclear Information System (INIS)

2005-01-01

Technologically-reinforced natural radioactivity comes from mining industries, geological resources and ores de-confinement, and from separation, purification, transformation and use of by-products or products. Partly based on a survey and questionnaires sent to industrial organisations, this report proposes a large and detailed overview of this kind of radioactivity for different sectors or specific activities: the French phosphate sector, the international rare Earth and heavy ores sector, the French monazite sector, the ilmenite sector, the French and international zirconium sector, the non-ferrous metal sector, the international and French drinkable, mineral and spring water sector, the international wastewater sector, the French drilling sector, the international and French geothermal sector, the international and French gas and oil sector, the international and French coal sector, the international and French biomass sector, the international and French paper-making industry, and the management of wastes with technologically-reinforced natural radioactivity in France

Teaching concepts in the field of radioactivity and nuclear energy

International Nuclear Information System (INIS)

Bleichroth, W.

1982-01-01

This issue of 'Naturwissenschaften im Unterricht', dealing with the subjects of physics and chemistry, gives an account of the fundamentals of radioactivity, nuclear fission, controlled nuclear fission, nuclear power plants, dosimetry, safety engineering, shielding measures to prevent the release of radioactive substances, pressure engineering, emergency core cooling systems, waste disposal and natural and artificial environmental radioactivity. Teachers will find helpful hints as to the method of instruction and the explanation of problems. (DG) [de

Noether symmetry for non-minimally coupled fermion fields

International Nuclear Information System (INIS)

Souza, Rudinei C de; Kremer, Gilberto M

2008-01-01

A cosmological model where a fermion field is non-minimally coupled with the gravitational field is studied. By applying Noether symmetry the possible functions for the potential density of the fermion field and for the coupling are determined. Cosmological solutions are found showing that the non-minimally coupled fermion field behaves as an inflaton describing an inflationary scenario, whereas the minimally coupled fermion field describes a decelerated period, behaving as a standard matter field

Low-waste technology of prevention, decontamination and localization of radioactive contamination

International Nuclear Information System (INIS)

Kizhnerov, L. V.; Konstantinov, Ye. A.; Prokopenko, V. A.; Sorokin, N. M.

1997-01-01

The report presents the results of research in developing a low-waste technology of prevention, decontamination and localization of radioactive contamination founded on the of easily removed protective polymeric coating based on water and alcohol latexes and dispersion of polymers with special activating additives. The developed technology provides for the reduction of weakly fixed radioactive contamination of non-painted and painted surfaces to admissible levels (as a rule), it securely prevents and localizes contamination and does not generate secondary liquid radioactive wastes

[Safety Evaluation of Rare Sugar Syrup: Single-dose Oral Toxicity in Rats, Reverse Mutation Assay, Chromosome Aberration Assay, and Acute Non-Effect Level for Diarrhea of a Single Dose in Humans].

Science.gov (United States)

Yamada, Takako; Iida, Tetsuo; Takamine, Satoshi; Hayashi, Noriko; Okuma, Kazuhiro

2015-01-01

The safety of rare sugar syrup obtained from high-fructose corn syrup under slightly alkaline conditions was studied. Mutagenicity of rare sugar syrup was assessed by a reverse mutation assay using Salmonella typhimurium and Escherichia coli, and an in vitro chromosomal aberration assay using Chinese hamster lung cell line (CHL/IU). No mutagenicity of rare sugar syrup was detected under these experimental conditions. Oral administration of single dose (15,000 mg/kg) of rare sugar syrup to rats caused no abnormalities, suggesting no adverse effect of rare sugar syrup. In humans, the acute non-effect level of rare sugar syrup for causing diarrhea was estimated as 0.9 g/kg body weight as dry solid base in both males and females.

Modeling and risk assessment of a 30-Year-old subsurface radioactive-liquid drain field

Science.gov (United States)

Dawson, Lon A.; Pohl, Phillip I.

1997-11-01

The contamination from a 30-year-old radioactive liquid drain field was assessed for movement in the subsurface and potential risks to humans. This assessment included determining field concentrations of cesium 137 (137Cs) and other inorganic contaminants and modeling of the flow and transport of the liquid waste that was sent to the drain field. The field investigation detected no contamination deeper than 15 feet (4.6 m) from the bottom of the drain field. Prediction of the water content of the vadose zone showed no saturated conditions for times greater than 10 years after the known infiltration. Sensitivity analysis of the modeling parameters showed the equilibrium sorption coefficient to be the most important factor in predicting the contaminant plumes. Calibration of modeling results with field data gave a 137Cs sorption coefficient that is within the range of values found in the literature. The risk assessment for the site showed that the contamination poses no significant risk to human health.

Non-linear degradation model of cement barriers in a borehole repository for disused radioactive sources

International Nuclear Information System (INIS)

Gharbieh, Heidar K.; Cota, Stela

2015-01-01

Narrow diameter borehole facilities (a few tens of centimeters), like the BOSS concept developed by the IAEA, provide a safe and cost effective disposal option for radioactive waste and particularly disused sources. The BOSS concept (borehole disposal of sealed radioactive sources) comprises a multi-barrier system of cement grout and stainless steel components. In order to predict the long-time performance of the cement barriers as an input of a future safety assessment under the specific hydrochemical and hydrological conditions, a non-linear degradation model was developed in this work. With the assistance of the program 'PHREEQC' it describes the change of the porosity and the hydraulic conductivity with time, which also let to conclusions concerning the change of the sorption capacity of the cement grout. This work includes the theoretical approach and illustrates the non-liner degradation by means of an exemplary water composition found in the saturated zone and the dimensions of the backfill made of cement grout representing a barrier of the BOSS borehole facility. (author)

Towards a high throughput droplet-based agglutination assay

KAUST Repository

Kodzius, Rimantas; Castro, David; Foulds, Ian G.

2013-01-01

This work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.

Towards a high throughput droplet-based agglutination assay

KAUST Repository

Kodzius, Rimantas

2013-10-22

This work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.

Inspection with non destructive assay techniques of the aluminium coating of the TRIGA Mark III reactor vat

International Nuclear Information System (INIS)

Reyes A, A.I.; Gonzalez M, A.; Castaneda J, G.; Rivera M, H.; Sandoval G, I.

2001-01-01

In June 2000, the Reactor Department assigned to the Scientific Research Direction of the National Institute of Nuclear Research requested to the Non-destructive Assays Laboratory (LEND), assigned to the Materials Science Management, the inspection and measurement of thickness of the aluminium coating (liner) of the TRIGA Mark III reactor vat with non-destructive assay techniques, due to that the aluminium coating is exposed mainly to undergo slimming on its back side due to corrosion phenomena. Activity that was able to be carried out from april until august 2001. It is worth pointing out that this type of inspection with these techniques was realized by first time. The non-destructive assays (NDA) are techniques which use indirect physical methods for inspecting the sanitation of components in process or in service, for detect lack of continuity or defects which affect their quality or usefulness. The application of those do not alter the physical, chemical, mechanical or dimensional properties of the part subject of inspection. The results of the application of the ultrasound inspection techniques, industrial radiography and penetrating liquids are presented. (Author)

Numerical analysis of thermal process in the near field around vertical disposal of high-level radioactive waste

Directory of Open Access Journals (Sweden)

H.G. Zhao

2014-02-01

Full Text Available For deep geological disposal of high-level radioactive waste (HLW in granite, the temperature on the HLW canisters is commonly designed to be lower than 100 °C. This criterion dictates the dimension of the repository. Based on the concept of HLW disposal in vertical boreholes, thermal process in the near field (host rock and buffer surrounding HLW canisters has been simulated by using different methods. The results are drawn as follows: (a the initial heat power of HLW canisters is the most important and sensitive parameter for evolution of temperature field; (b the thermal properties and variations of the host rock, the engineered buffer, and possible gaps between canister and buffer and host rock are the additional key factors governing the heat transformation; (c the gaps width and the filling by water or air determine the temperature offsets between them.

Simplified scheme or radioactive plume calculations

International Nuclear Information System (INIS)

Gibson, T.A.; Montan, D.N.

1976-01-01

A simplified mathematical scheme to estimate external whole-body γ radiation exposure rates from gaseous radioactive plumes was developed for the Rio Blanco Gas Field Nuclear Stimulation Experiment. The method enables one to calculate swiftly, in the field, downwind exposure rates knowing the meteorological conditions and γ radiation exposure rates measured by detectors positioned near the plume source. The method is straightforward and easy to use under field conditions without the help of mini-computers. It is applicable to a wide range of radioactive plume situations. It should be noted that the Rio Blanco experiment was detonated on May 17, 1973, and no seep or release of radioactive material occurred

Radioactive waste management in Mexico

International Nuclear Information System (INIS)

Paredes, L.; Reyes L, J.; Jimenez D, J.

2000-01-01

This paper describes the radioactive waste management in Mexico, particularly the activities that the National Institute of Nuclear Research (NINR) is undertaking in this field. Classification and annual generation of radioactive waste, together with practices and facilities relating to the management of radioactive waste are addressed. The respective national legal framework and policy are outlined. (author)

Emerging concepts and requirements for the long-term management of non-radioactive hazardous wastes - would geological disposal be an appropriate solution for some of these wastes

International Nuclear Information System (INIS)

Rein, K. von

1994-01-01

This work deals with the emerging concepts and requirements for the long-term management of non-radioactive hazardous wastes. After some generalities on the pollution of natural environment and the legislations taken by the swedish government the author tries to answer to the question : would geological disposal be an appropriate solution for the non-radioactive hazardous wastes? Then is given the general discussion of the last three articles concerning the background to current environmental policies and their implementation and more particularly the evolution and current thoughts about environmental policies, the managing hazardous activities and substances and the emerging concepts and requirements for the long-term management of non-radioactive hazardous wastes. Comments and questions concerning the similarity or otherwise between the present position of radioactive waste disposal and the background to current environmental policies are indicated. (O.L.)

Multiplex PCR-based assay for detection of Bordetella pertussis in nasopharyngeal swab specimens.

Science.gov (United States)

Wadowsky, R M; Michaels, R H; Libert, T; Kingsley, L A; Ehrlich, G D

1996-11-01

A multiplex PCR-based assay was developed for the detection of Bordetella pertussis in nasopharyngeal swab specimens. The assay simultaneously amplified two separate DNA targets (153 and 203 bp) within a B. pertussis repetitive element and a 438-bp target within the beta-actin gene of human DNA (PCR amplification control). PCR products were detected by a sensitive and specific liquid hybridization gel retardation assay. A total of 496 paired nasopharyngeal swab specimens were tested by both the PCR-based assay and culture. Although 30 (6%) of the specimens inhibited the amplification of the beta-actin target, in all 29 specimens studied, the inhibition disappeared on repeat testing or was easily overcome with a 1:8 dilution or less of specimen digest. Of the 495 specimen pairs yielding a final evaluable result by the PCR-based assay, 19.0% were positive by the PCR-based assay, whereas 13.9% were positive by culture (P < 0.0001). After resolving the PCR-positive, culture-negative results by testing an additional aliquot from these specimens by the multiplex PCR-based assay, the PCR-based assay had a sensitivity and specificity of 98.9 and 99.7%, respectively, compared with values of 73.4 and 100%, respectively, for culture. In comparison with patients with culture-confirmed pertussis, those with PCR-positive, culture-negative results were older and more likely to have had prolonged cough, immunization with pertussis vaccine, or treatment with erythromycin. This multiplex PCR-based assay is substantially more sensitive than culture and identifies specimens that contain inhibitors of PCR.

System for disposing of radioactive waste

International Nuclear Information System (INIS)

Gablin, K.A.; Hansen, L.J.

1979-01-01

A system is described for disposing of radioactive waste material from nuclear reactors by solidifying the liquid components to produce an encapsulated mass adapted for disposal by burial. The method contemplates mixing of radioactive waste materials, with or without contained solids, with a setting agent capable of solidifying the waste liquids into a free standing hardened mass, placing the resulting liquid mixture in a container with a proportionate amount of a curing agent to effect solidification under controlled conditions, and thereafter burying the container and contained solidified mixture. The setting agent is a water-extendable polymer consisting of a suspension of partially polymerized particles of urea formaldehyde in water, and the curing agent is sodium bisulfate. Methods are disclosed for dewatering slurry-like mixtures of liquid and particulate radioactive waste materials, such as spent ion exchange resin beads, and for effecting desired distribution of non-liquid radioactive materials in the central area of the container prior to solidification, so that the surrounding mass of lower specific radioactivity acts as a partial shield against higher radioactivity of the non-liquid radioactive materials. The methods also provide for addition of non-radioactive filler materials to dilute the mixture and lower the overall radioactivity of the hardened mixture to desired Lowest Specific Activity counts. An inhibiting agent is added to the liquid mixture to adjust the solidification time, and provision is made for adding additional amounts of setting agent and curing agent to take up any free water and further encapsulate the hardened material within the container. 30 claims

System for disposing of radioactive waste

International Nuclear Information System (INIS)

Gablin, K.A.; Hansen, L.J.

1977-01-01

A system is described for disposing of radioactive waste material from nuclear reactors by solidifying the liquid components to produce an encapsulated mass adapted for disposal by burial. The method contemplates mixing of radioactive waste materials, with or without contained solids, with a setting agent capable of solidifying the waste liquids into a free standing hardened mass, placing the resulting liquid mixture in a container with a proportionate amount of a curing agent to effect solidification under controlled conditions, and thereafter burying the container and contained solidified mixture. The setting agent is a water-extendable polymer consisting of a suspension of partially polymerized particles of urea formaldehyde in water, and the curing agent is sodium bisulfate. Methods are disclosed for dewatering slurry-like mixtures of liquid and particulate radioactive waste materials, such as spent ion exchange resin beads, and for effecting desired distribution of non-liquid radioactive materials in the central area of the container prior to solidification, so that the surrounding mass of lower specific radioactivity acts as a partial shield against higher radioactivity of the non-liquid radioactive materials. The methods also provide for addition of non-radioactive filler materials to dilute the mixture and lower the overall radioactivity of the hardened mixture to desired Lowest Specific Activity counts. An inhibiting agent is added to the liquid mixture to adjust the solidification time, and provision is made for adding additional amounts of setting agent and curing agent to take up any free water and further encapsulate the hardened material within the container

Non destructive assay of nuclear LEU spent fuels for burnup credit application

International Nuclear Information System (INIS)

Lebrun, A.; Bignan, G.

2001-01-01

Criticality safety analysis devoted to spent fuel storage and transportation has to be conservative in order to be sure no accident will ever happen. In the spent fuel storage field, the assumption of freshness has been used to achieve the conservative aspect of criticality safety procedures. Nevertheless, after being irradiated in a reactor core, the fuel elements have obviously lost part of their original reactivity. The concept of taking into account this reactivity loss in criticality safety analysis is known as Burnup credit. To be used, Burnup credit involves obtaining evidence of the reactivity loss with a Burnup measurement. Many non destructive assays (NDA) based on neutron as well as on gamma ray emissions are devoted to spent fuel characterization. Heavy nuclei that compose the fuels are modified during irradiation and cooling. Some of them emit neutrons spontaneously and the link to Burnup is a power link. As a result, burn-up determination with passive neutron measurement is extremely accurate. Some gamma emitters also have interesting properties in order to characterize spent fuels but the convenience of the gamma spectrometric methods is very dependent on characteristics of spent fuel. In addition, contrary to the neutron emission, the gamma signal is mostly representative of the peripheral rods of the fuels. Two devices based on neutron methods but combining different NDA methods which have been studied in the past are described in detail: 1. The PYTHON device is a combination of a passive neutron measurement, a collimated total gamma measurement, and an online depletion code. This device, which has been used in several Nuclear Power Plants in western Europe, gives the average Burnup within a 5% uncertainty and also the extremity Burnup, 2. The NAJA device is an automatic device that involves three nuclear methods and an online depletion code. It is designed to cover the whole fuel assembly panel (Active Neutron Interrogation, Passive Neutron

Environmental radioactivity and radiation exposure in 2015; Umweltradioaktivitaet und Strahlenbelastung im Jahr 2015

Energy Technology Data Exchange (ETDEWEB)

NONE

2017-07-20

The information of the German Federal Government on the environmental radioactivity and radiation exposure in 2015 covers the following issues: selected topics of radiation protection, natural radiation exposure; civilizing (artificial) radiation exposure: nuclear power plants and other nuclear facilities, uranium mine recultivation, radioactive materials in industry and households, fallout from nuclear weapon testing and reactor accidents; occupational radiation exposure: exposed personnel in nuclear facilities, aviation personnel, radiation accidents; medical radiation exposure: nuclear medical diagnostics and therapy; non-ionizing radiation: electromagnetic fields, UV radiation, optical radiation.

Towers of Function Fields over Non-prime Finite Fields

DEFF Research Database (Denmark)

Bassa, Alp; Beelen, Peter; Garcia, Arnaldo

2015-01-01

Over all non-prime finite fields, we construct some recursive towers of function fields with many rational places. Thus we obtain a substantial improvement on all known lower bounds for Ihara’s quantity A(ℓ), for ℓ = pn with p prime and n > 3 odd. We relate the explicit equations to Drinfeld modu...

Learning more about radioactivity

International Nuclear Information System (INIS)

2008-01-01

This digest brochure explains what radioactivity is, where it comes from, how it is measured, what are its effects on the body and the way to protect it against these effects, the uses of radioactivity (In the medical field, In industry, In the food industry, and In the cultural world). It ends with some examples of irradiation levels, of natural radioactivity and with the distribution in France of various sources of exposure. (J.S.)

Substrate coated with receptor and labelled ligand for assays

International Nuclear Information System (INIS)

1980-01-01

Improvements in the procedures for assaying ligands are described. The assay consists of a polystyrene tube on which receptors are present for both the ligand to be assayed and a radioactively labelled form of the ligand. The receptors on the bottom portion of the tube are also coated with labelled ligands, thus eliminating the necessity for separate addition of the labelled ligand and sample during an assay. Examples of ligands to which this method is applicable include polypeptides, nucleotides, nucleosides and proteins. Specific examples are given in which the ligand to be assayed is digoxin, the labelled form of the ligand is 3-0-succinyl digoxyigenin tyrosine ( 125 I) and the receptor is digoxin antibody. (U.K.)

Induced radioactivity at CERN

CERN Multimedia

1970-01-01

A description of some of the problems and some of the advantages associated with the phenomenon of induced radioactivity at accelerator centres such as CERN. The author has worked in this field for several years and has recently written a book 'Induced Radioactivity' published by North-Holland.

Diagnosis of HEV infection by serological and real-time PCR assays: a study on acute non-A-C hepatitis collected from 2004 to 2010 in Italy

Directory of Open Access Journals (Sweden)

Candido Angela

2012-06-01

Full Text Available Abstract Background The impact of hepatitis E in developed countries, like Italy, still requires a clear definition. In the present study, we evaluated HEV infection in patients with acute non-A-C hepatitis by an approach comparing data from Real-time PCR and serological assays. Methods In a first analysis, sera from 52 patients hospitalized with a diagnosis of acute viral non-A-C hepatitis in Italy were tested by in-house Real-Time PCR assay for identification of Hepatitis E Virus (HEV RNA and by anti-HEV IgM and IgG assays. In a subsequent analysis, selected samples were evaluated by additional IgM tests to confirm diagnosis. Results Among the 52 samples, 21 showed positive results for all three markers (IgM, IgG and HEV RNA. One patient showed HEV RNA as single marker. Uncertain results were found in 8 samples while the remaining 22 were negative for all markers. Further analysis of the 8 undefined samples by additional IgM tests confirmed HEV infection in 1 patient. Overall, acute HEV infections were reliably identified in 23 (44.2% out of 52 patients. Conclusions In the present paper, we performed a study evaluating HEV infection in 52 sporadic non-A-C acute hepatitis cases. All samples were collected from 2004 to 2010 in Italy. By a diagnostic strategy based on genomic and serological assays we identified HEV infections in 23 out of 52 patients (44.2%, a percentage higher than previous estimates. Thus, the actual impact of HEV infections in Italy needs to be further evaluated on a national scale by a diagnostic strategy based on multiple and last generation assays.

A new seed-based assay for meiotic recombination in Arabidopsis thaliana.

NARCIS (Netherlands)

Melamed-Bessudo, C.; Yehuda, E.; Stuitje, A.R.; Levy, A.A.

2005-01-01

Meiotic recombination is a fundamental biological process that plays a central role in the evolution and breeding of plants. We have developed a new seed-based assay for meiotic recombination in Arabidopsis. The assay is based on the transformation of green and red fluorescent markers expressed

Random assay in radioimmunoassay: Feasibility and application compared with batch assay

Energy Technology Data Exchange (ETDEWEB)

Lee, Jung Min; Lee, Hwan Hee; Park, Sohyun; Kim, Tae Sung; Kim, Seok Ki [Dept. of Nuclear MedicineNational Cancer Center, Goyang (Korea, Republic of)

2016-12-15

The batch assay has been conventionally used for radioimmunoassay (RIA) because of its technical robustness and practical convenience. However, it has limitations in terms of the relative lag of report time due to the necessity of multiple assays in a small number of samples compared with the random assay technique. In this study, we aimed to verify whether the random assay technique can be applied in RIA and is feasible in daily practice. The coefficients of variation (CVs) of eight standard curves within a single kit were calculated in a CA-125 immunoradiometric assay (IRMA) for the reference of the practically ideal CV of the CA-125 kit. Ten standard curves of 10 kits from 2 prospectively collected lots (pLot) and 85 standard curves of 85 kits from 3 retrospectively collected lots (Lot) were obtained. Additionally, the raw measurement data of both 170 control references and 1123 patients' sera were collected retrospectively between December 2015 and January 2016. A standard curve of the first kit of each lot was used as a master standard curve for a random assay. The CVs of inter-kits were analyzed in each lot, respectively. All raw measurements were normalized by decay and radioactivity. The CA-125 values from control samples and patients' sera were compared using the original batch assay and random assay. In standard curve analysis, the CVs of inter-kits in pLots and Lots were comparable to those within a single kit. The CVs from the random assay with normalization were similar to those from the batch assay in the control samples (CVs % of low/high concentration; Lot1 2.71/1.91, Lot2 2.35/1.83, Lot3 2.83/2.08 vs. Lot1 2.05/1.21, Lot2 1.66/1.48, Lot3 2.41/2.14). The ICCs between the batch assay and random assay using patients' sera were satisfactory (Lot1 1.00, Lot2 0.999, Lot3 1.00). The random assay technique could be successfully applied to the conventional CA-125 IRMA kits. The random assay showed strong agreement with the batch assay. The

Radioactive waste management and their ultimate disposal technologies in India: recent practices and developments

International Nuclear Information System (INIS)

Bajpai, R.K.

2015-01-01

The radioactive wastes that arise from the use of uranium, thorium and other radioactive isotopes during their various applications in the field of power generation, industry, agriculture and medicine are among the most talked about and at time even highly dreaded substances created by man in recent past. Most of the fears owe their genesis to extraordinary periods of time over which such waste remain radioactive and continue to emit radiations which are invisible to human eyes. These time periods run into tens of thousands of years and as a result any solution for management and permanent isolation of such waste needs to demonstrate safety of mankind and environment over such lengthy time frames. For example a radioactive isotope of strontium, known as 90 Sr takes about 300 years to loose its entire radioactivity. Similarly a popular isotope of plutonium 239 Pu needs almost 2.5 lakhs years to shed its entire radioactivity by converting itself to a non radioactive element. In this talk some salient aspects of radioactive waste management and solutions for their permanent isolation under consideration in India are discussed

Cluster radioactive decay within the preformed cluster model using relativistic mean-field theory densities

International Nuclear Information System (INIS)

Singh, BirBikram; Patra, S. K.; Gupta, Raj K.

2010-01-01

We have studied the (ground-state) cluster radioactive decays within the preformed cluster model (PCM) of Gupta and collaborators [R. K. Gupta, in Proceedings of the 5th International Conference on Nuclear Reaction Mechanisms, Varenna, edited by E. Gadioli (Ricerca Scientifica ed Educazione Permanente, Milano, 1988), p. 416; S. S. Malik and R. K. Gupta, Phys. Rev. C 39, 1992 (1989)]. The relativistic mean-field (RMF) theory is used to obtain the nuclear matter densities for the double folding procedure used to construct the cluster-daughter potential with M3Y nucleon-nucleon interaction including exchange effects. Following the PCM approach, we have deduced empirically the preformation probability P 0 emp from the experimental data on both the α- and exotic cluster-decays, specifically of parents in the trans-lead region having doubly magic 208 Pb or its neighboring nuclei as daughters. Interestingly, the RMF-densities-based nuclear potential supports the concept of preformation for both the α and heavier clusters in radioactive nuclei. P 0 α(emp) for α decays is almost constant (∼10 -2 -10 -3 ) for all the parent nuclei considered here, and P 0 c(emp) for cluster decays of the same parents decrease with the size of clusters emitted from different parents. The results obtained for P 0 c(emp) are reasonable and are within two to three orders of magnitude of the well-accepted phenomenological model of Blendowske-Walliser for light clusters.

One-step fabrication of nanostructure-covered microstructures using selective aluminum anodization based on non-uniform electric field

Science.gov (United States)

Park, Yong Min; Kim, Byeong Hee; Seo, Young Ho

2016-06-01

This paper presents a selective aluminum anodization technique for the fabrication of microstructures covered by nanoscale dome structures. It is possible to fabricate bulging microstructures, utilizing the different growth rates of anodic aluminum oxide in non-uniform electric fields, because the growth rate of anodic aluminum oxide depends on the intensity of electric field, or current density. After anodizing under a non-uniform electric field, bulging microstructures covered by nanostructures were fabricated by removing the residual aluminum layer. The non-uniform electric field induced by insulative micropatterns was estimated by computational simulations and verified experimentally. Utilizing computational simulations, the intensity profile of the electric field was calculated according to the ratio of height and width of the insulative micropatterns. To compare computational simulation results and experimental results, insulative micropatterns were fabricated using SU-8 photoresist. The results verified that the shape of the bottom topology of anodic alumina was strongly dependent on the intensity profile of the applied electric field, or current density. The one-step fabrication of nanostructure-covered microstructures can be applied to various fields, such as nano-biochip and nano-optics, owing to its simplicity and cost effectiveness.

A kinematic-based methodology for radiological protection: Runoff analysis to calculate the effective dose for internal exposure caused by ingestion of radioactive isotopes

Science.gov (United States)

Sasaki, Syota; Yamada, Tadashi; Yamada, Tomohito J.

2014-05-01

We aim to propose a kinematic-based methodology similar with runoff analysis for readily understandable radiological protection. A merit of this methodology is to produce sufficiently accurate effective doses by basic analysis. The great earthquake attacked the north-east area in Japan on March 11, 2011. The system of electrical facilities to control Fukushima Daiichi nuclear power plant was completely destroyed by the following tsunamis. From the damaged reactor containment vessels, an amount of radioactive isotopes had leaked and been diffused in the vicinity of the plant. Radiological internal exposure caused by ingestion of food containing radioactive isotopes has become an issue of great interest to the public, and has caused excessive anxiety because of a deficiency of fundamental knowledge concerning radioactivity. Concentrations of radioactivity in the human body and internal exposure have been studied extensively. Previous radiologic studies, for example, studies by International Commission on Radiological Protection(ICRP), employ a large-scale computational simulation including actual mechanism of metabolism in the human body. While computational simulation is a standard method for calculating exposure doses among radiology specialists, these methods, although exact, are too difficult for non-specialists to grasp the whole image owing to the sophistication. In this study, the human body is treated as a vessel. The number of radioactive atoms in the human body can be described by an equation of continuity, which is the only governing equation. Half-life, the period of time required for the amount of a substance decreases by half, is only parameter to calculate the number of radioactive isotopes in the human body. Half-life depends only on the kinds of nuclides, there are no arbitrary parameters. It is known that the number of radioactive isotopes decrease exponentially by radioactive decay (physical outflow). It is also known that radioactive isotopes

Risk based regulation: a convenient concept for legislation and regulation in the field of technical risks?

International Nuclear Information System (INIS)

Seiler, J.H.

1998-01-01

Legislation and regulation concerning risk activities are traditionally based on deterministic safety measures. This may lead to inefficient results: sometimes the law requires safety measures which are - from an economic viewpoint - not justified because of their poor cost-effectiveness; sometimes it does not require safety measures although they would be very efficient. The risk based regulation approach wants to make the law more efficient and to get more safety at less costs. Legislation and regulation should be based on terms of risk rather than on deterministic rules. Risk should be expressed in quantitative terms and risk regulation should be based on the cost-effectiveness of safety measures. Thus a most efficient (in the sense of the economic analysis of the law) strategy for safety and environmental law could be established. The approach is economically reasonable and theoretically convincing. Its practical implementation however raises a lot of technical and legal questions. The project 'Risk Based Regulation' (1996-1999), sponsored by the Swiss National Fund for Scientific Research, intends to evaluate the practical feasibility of the approach from a technical and a legal view. It contains a general part which describes the risk based regulation approach and its legal and technical questions, case studies which try to practically implement the risk based regulation approach; the case studies are: storage and management of explosives in the army, storage and management of explosives for non-military purposes, safety at work, accident prevention in the non-professional field (mainly road accidents), fire protection, transportation of dangerous goods, waste disposal: traditional waste, waste disposal: radioactive waste, nuclear energy (reactor safety), a synthesis with recommendations for the future legislation and regulation in the field of technical risks. The paper presents the project and its preliminary results. (author)

Particle size of radioactive aerosols generated during machine operation in high-energy proton accelerators

International Nuclear Information System (INIS)

Oki, Yuichi; Kanda, Yukio; Kondo, Kenjiro; Endo, Akira

2000-01-01

In high-energy accelerators, non-radioactive aerosols are abundantly generated due to high radiation doses during machine operation. Under such a condition, radioactive atoms, which are produced through various nuclear reactions in the air of accelerator tunnels, form radioactive aerosols. These aerosols might be inhaled by workers who enter the tunnel just after the beam stop. Their particle size is very important information for estimation of internal exposure doses. In this work, focusing on typical radionuclides such as 7 Be and 24 Na, their particle size distributions are studied. An aluminum chamber was placed in the EP2 beam line of the 12-GeV proton synchrotron at High Energy Accelerator Research Organization (KEK). Aerosol-free air was introduced to the chamber, and aerosols formed in the chamber were sampled during machine operation. A screen-type diffusion battery was employed in the aerosol-size analysis. Assuming that the aerosols have log-normal size distributions, their size distributions were obtained from the radioactivity concentrations at the entrance and exit of the diffusion battery. Radioactivity of the aerosols was measured with Ge detector system, and concentrations of non-radioactive aerosols were obtained using condensation particle counter (CPC). The aerosol size (radius) for 7 Be and 24 Na was found to be 0.01-0.04 μm, and was always larger than that for non-radioactive aerosols. The concentration of non-radioactive aerosols was found to be 10 6 - 10 7 particles/cm 3 . The size for radioactive aerosols was much smaller than ordinary atmospheric aerosols. Internal doses due to inhalation of the radioactive aerosols were estimated, based on the respiratory tract model of ICRP Pub. 66. (author)

Radioactive waste and transport. Chapter 6

International Nuclear Information System (INIS)

1978-01-01

A brief definition of the nature of radioactive waste is followed by a more detailed discussion of high level waste, its composition the amounts involved, storage in liquid and in solid form and the storage of non-reprocessed spent fuel. The final disposal of high level waste in deep geological structures is then described, based on the Swedish KBS study. The effectiveness of the artificial and natural barriers in preventing the radioactive substances from reaching the biosphere is discussed. American and Swedish risk analyses are briefly discussed, and practical experience presented. Low and medium level wastes are thereafter treated in a similar, though briefer manner. Transport of radioactive materials, fresh fuel, spent fuel and waste is then dealt with. Regulations for the containers and their tests are briefly presented and the risk of accidents, theft and sabotage during transport are discussed. (JIW)

Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae.

Science.gov (United States)

Bobosha, Kidist; Tjon Kon Fat, Elisa M; van den Eeden, Susan J F; Bekele, Yonas; van der Ploeg-van Schip, Jolien J; de Dood, Claudia J; Dijkman, Karin; Franken, Kees L M C; Wilson, Louis; Aseffa, Abraham; Spencer, John S; Ottenhoff, Tom H M; Corstjens, Paul L A M; Geluk, Annemieke

2014-05-01

Field-applicable tests detecting asymptomatic Mycobacterium leprae (M. leprae) infection or predicting progression to leprosy, are urgently required. Since the outcome of M. leprae infection is determined by cellular- and humoral immunity, we aim to develop diagnostic tests detecting pro-/anti-inflammatory and regulatory cytokines as well as antibodies against M. leprae. Previously, we developed lateral flow assays (LFA) for detection of cytokines and anti-PGL-I antibodies. Here we evaluate progress of newly developed LFAs for applications in resource-poor settings. The combined diagnostic value of IP-10, IL-10 and anti-PGL-I antibodies was tested using M. leprae-stimulated blood of leprosy patients and endemic controls (EC). For reduction of the overall test-to-result time the minimal whole blood assay time required to detect distinctive responses was investigated. To accommodate LFAs for field settings, dry-format LFAs for IP-10 and anti-PGL-I antibodies were developed allowing storage and shipment at ambient temperatures. Additionally, a multiplex LFA-format was applied for simultaneous detection of anti-PGL-I antibodies and IP-10. For improved sensitivity and quantitation upconverting phosphor (UCP) reporter technology was applied in all LFAs. Single and multiplex UCP-LFAs correlated well with ELISAs. The performance of dry reagent assays and portable, lightweight UCP-LF strip readers indicated excellent field-robustness. Notably, detection of IP-10 levels in stimulated samples allowed a reduction of the whole blood assay time from 24 h to 6 h. Moreover, IP-10/IL-10 ratios in unstimulated plasma differed significantly between patients and EC, indicating the feasibility to identify M. leprae infection in endemic areas. Dry-format UCP-LFAs are low-tech, robust assays allowing detection of relevant cytokines and antibodies in response to M. leprae in the field. The high levels of IP-10 and the required shorter whole blood assay time, render this cytokine useful to

Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae.

Directory of Open Access Journals (Sweden)

Kidist Bobosha

2014-05-01

Full Text Available BACKGROUND: Field-applicable tests detecting asymptomatic Mycobacterium leprae (M. leprae infection or predicting progression to leprosy, are urgently required. Since the outcome of M. leprae infection is determined by cellular- and humoral immunity, we aim to develop diagnostic tests detecting pro-/anti-inflammatory and regulatory cytokines as well as antibodies against M. leprae. Previously, we developed lateral flow assays (LFA for detection of cytokines and anti-PGL-I antibodies. Here we evaluate progress of newly developed LFAs for applications in resource-poor settings. METHODS: The combined diagnostic value of IP-10, IL-10 and anti-PGL-I antibodies was tested using M. leprae-stimulated blood of leprosy patients and endemic controls (EC. For reduction of the overall test-to-result time the minimal whole blood assay time required to detect distinctive responses was investigated. To accommodate LFAs for field settings, dry-format LFAs for IP-10 and anti-PGL-I antibodies were developed allowing storage and shipment at ambient temperatures. Additionally, a multiplex LFA-format was applied for simultaneous detection of anti-PGL-I antibodies and IP-10. For improved sensitivity and quantitation upconverting phosphor (UCP reporter technology was applied in all LFAs. RESULTS: Single and multiplex UCP-LFAs correlated well with ELISAs. The performance of dry reagent assays and portable, lightweight UCP-LF strip readers indicated excellent field-robustness. Notably, detection of IP-10 levels in stimulated samples allowed a reduction of the whole blood assay time from 24 h to 6 h. Moreover, IP-10/IL-10 ratios in unstimulated plasma differed significantly between patients and EC, indicating the feasibility to identify M. leprae infection in endemic areas. CONCLUSIONS: Dry-format UCP-LFAs are low-tech, robust assays allowing detection of relevant cytokines and antibodies in response to M. leprae in the field. The high levels of IP-10 and the required

Comparison of flow cytometry and immunohistochemistry in non-radioisotopic murine lymph node assay using bromodeoxyuridine.

Science.gov (United States)

Jung, Kyoung-Mi; Bae, Il-Hong; Kim, Bae-Hwan; Kim, Wang-Ki; Chung, Jin-Ho; Park, Young-Ho; Lim, Kyung-Min

2010-02-01

Non-radioisotopic local lymph node assay (LLNA) employing 5-bromo-2'-deoxyuridine (BrdU) with flow cytometry (FACS) or immunohistochemistry (IHC) is gaining attention due to a regulatory issue of using radioisotope, (3)H-thymidine, in vivo in traditional LLNA. In this study, to compare the performance of these non-radioisotopic endpoints, 7 chemicals with known sensitizing potencies were examined in LLNA. Mice were topically treated with chemicals or vehicle on both ears for 3 days. After intraperitoneal injection of BrdU, bilateral lymph nodes were isolated separately and undergone respectively, FACS or IHC to determine BrdU incorporated lymph node cells (LNCs). Weight and histology of treated ears were also examined to evaluate chemical-induced edema and irritation. Both FACS and IHC could successively identify the skin sensitizers from non-sensitizers. Comparison of FACS and IHC with traditional LLNA revealed that FACS has a higher sensitivity although both assays produced comparable sensitivity and performance to traditional LLNA. In conclusion, non-radioisotopic LLNA using FACS and IHC can successfully detect sensitizers with a good correlation to traditional LLNA. Notably, FACS showed almost equivalent sensitivity and accuracy to traditional LLNA. 2009 Elsevier Ireland Ltd. All rights reserved.

PROMETHEE: a versatile R and D measurement device for low level waste assay

International Nuclear Information System (INIS)

Romeyer Dherby, J.; Passard, C.; Mariani, A.

1996-01-01

The accurate measurement of heavy nuclide masses and activities in radioactive wastes drums is an important part of waste management. The Active/Passive non destructive assay of radioactive waste drums using a 14 MeV neutron generator is particularly interesting for alpha low level measurements or for gamma irradiating wastes. The development, optimisation, and validation of such a device for industrial use necessitate the building of a demonstrator. In 1985, the CEA decided to build at Cadarache the PROMETHEE modular system for experimenting the pulsed generator techniques, and since then, this device has led us to define several specific systems. At the present time, in the frame of COGEMA actions to reduce the volume of the reprocessing waste, a new strategy of drumming and incineration is going to start at LA HAGUE and MARCOULE, for the low level waste planned for surface storage. This strategy depends on the performance improvement of non destructive measurements systems used for the alpha waste evaluation. In this goal, a developments and tests are carried out on the PROMETHEE research and development facility at CEA CADARACHE, in order to obtain the required performances

PROMETHEE: a versatile R and D measurement device for low level waste assay

Energy Technology Data Exchange (ETDEWEB)

Romeyer Dherby, J.; Passard, C.; Mariani, A

1996-12-31

The accurate measurement of heavy nuclide masses and activities in radioactive wastes drums is an important part of waste management. The Active/Passive non destructive assay of radioactive waste drums using a 14 MeV neutron generator is particularly interesting for alpha low level measurements or for gamma irradiating wastes. The development, optimisation, and validation of such a device for industrial use necessitate the building of a demonstrator. In 1985, the CEA decided to build at Cadarache the PROMETHEE modular system for experimenting the pulsed generator techniques, and since then, this device has led us to define several specific systems. At the present time, in the frame of COGEMA actions to reduce the volume of the reprocessing waste, a new strategy of drumming and incineration is going to start at LA HAGUE and MARCOULE, for the low level waste planned for surface storage. This strategy depends on the performance improvement of non destructive measurements systems used for the alpha waste evaluation. In this goal, a developments and tests are carried out on the PROMETHEE research and development facility at CEA CADARACHE, in order to obtain the required performances.

Status of operation of radionuclides assay system in Korean nuclear power plant

International Nuclear Information System (INIS)

Hwang, K.H.; Lee, K.J.; Jeong, C.W.; Ahn, S.M.

2003-01-01

In Korea, 17 nuclear power plants composed of 13 pressurized water reactors and 4 CANDU reactors are currently in operation. The cumulative amounts of low and intermediate level radioactive waste in nuclear power plant reached 58,718 drums (unit: 200 liter) in 2001. Efforts to construct LILW disposal facility are continued and its first operation is planned in the year 2008. Its first stage capacity is assumed to be 100,000 drums and total capacity will reach to 800,000 drums. Radwaste disposal site selection is an urgent national project at present time. Regulations and guidelines require detailed information about the radioactive waste package and its contents prior to the transport to the disposal sites. The Enforcement Decree of the Korean Atomic Energy Act (articles 234-17) requires the Minister of Science and Technology (MOST) of Korea to establish regulation for the waste acceptance (MOST notice. 1196-10). It requires detailed information about the radioactive waste package and its contents such as activity of radionuclides, total activities, types and characteristics of waste. For the measurement of the concentrations and activities of radionuclides in radwaste drum, a radionuclides assay system is installed at Korean nuclear power plant (KORI site) in 1996. The waste drum can be measured in the vertical direction with eight vertical segments while in the radial direction also with eight segments. Using this measurement method, homogeneous and non-homogeneous waste drum can be measured. Scaling factor methods have been played a dominant role in the determination of the radionuclides concentration in this system. For corrosion product, generic scaling factors were used due to the similarity and better-characterized properties of Korean analyzed data as compared with the worldwide data base of PWR industry. For fission product and TRU nuclides, it is not easy to determine the generic scaling factors. Thus simple model reflecting the operation history of power

Receptor-based screening assays for the detection of antibiotics residues - A review.

Science.gov (United States)

Ahmed, Saeed; Ning, Jianan; Cheng, Guyue; Ahmad, Ijaz; Li, Jun; Mingyue, Liu; Qu, Wei; Iqbal, Mujahid; Shabbir, M A B; Yuan, Zonghui

2017-05-01

Consumer and regulatory agencies have a high concern to antibiotic residues in food producing animals, so appropriate screening assays of fast, sensitive, low cost, and easy sample preparation for the identification of these residues are essential for the food-safety insurance. Great efforts in the development of a high-throughput antibiotic screening assay have been made in recent years. Concerning the screening of antibiotic residue, this review elaborate an overview on the availability, advancement and applicability of antibiotic receptor based screening assays for the safety assessment of antibiotics usage (i.e. radio receptor assay, enzyme labeling assays, colloidal gold receptor assay, enzyme colorimetry assay and biosensor assay). This manuscript also tries to shed a light on the selection, preparation and future perspective of receptor protein for antibiotic residue detection. These assays have been introduced for the screening of numerous food samples. Receptor based screening technology for antibiotic detection has high accuracy. It has been concluded that at the same time, it can detect a class of drugs for certain receptor, and realize the multi-residue detection. These assays offer fast, easy and precise detection of antibiotics. Copyright © 2017 Elsevier B.V. All rights reserved.

Fusion reactor radioactive waste management

International Nuclear Information System (INIS)

Kaser, J.D.; Postma, A.K.; Bradley, D.J.

1976-01-01

Quantities and compositions of non-tritium radioactive waste are estimated for some current conceptual fusion reactor designs, and disposal of large amounts of radioactive waste appears necessary. Although the initial radioactivity of fusion reactor and fission reactor wastes are comparable, the radionuclides in fusion reactor wastes are less hazardous and have shorter half-lives. Areas requiring further research are discussed

Incorporation of Savannah River Plant radioactive waste into concrete

International Nuclear Information System (INIS)

Stone, J.A.

1975-01-01

Results are reported of a laboratory-scale experimental program at the Savannah River Laboratory to gain information on the fixation of high-level radioactive wastes in concrete. Two concrete formulations, a High-Alumina Cement and a Portland Pozzalanic cement, were selected on the bases of leachability and compressive strength for the fixation of non-radioactive simulated wastes. Therefore, these two cements were selected for current studies for the fixation of actual Savannah River Plant high-level wastes. (U.S.)

Transportation legislative data base: State radioactive materials transportation statute compilation, 1989--1993

International Nuclear Information System (INIS)

1994-04-01

The Transportation Legislative Data Base (TLDB) is a computer-based information service containing summaries of federal, state and certain local government statutes and regulations relating to the transportation of radioactive materials in the United States. The TLDB has been operated by the National Conference of State Legislatures (NCSL) under cooperative agreement with the US Department of Energy's (DOE) Office of Civilian Radioactive Waste Management since 1992. The data base system serves the legislative and regulatory information needs of federal, state, tribal and local governments, the affected private sector and interested members of the general public. Users must be approved by DOE and NCSL. This report is a state statute compilation that updates the 1989 compilation produced by Battelle Memorial Institute, the previous manager of the data base. This compilation includes statutes not included in the prior compilation, as well as newly enacted laws. Statutes not included in the prior compilation show an enactment date prior to 1989. Statutes that deal with low-level radioactive waste transportation are included in the data base as are statutes from the states of Alaska and Hawaii. Over 155 new entries to the data base are summarized in this compilation

Human neuron-astrocyte 3D co-culture-based assay for evaluation of neuroprotective compounds.

Science.gov (United States)

Terrasso, Ana Paula; Silva, Ana Carina; Filipe, Augusto; Pedroso, Pedro; Ferreira, Ana Lúcia; Alves, Paula Marques; Brito, Catarina

Central nervous system drug development has registered high attrition rates, mainly due to the lack of efficacy of drug candidates, highlighting the low reliability of the models used in early-stage drug development and the need for new in vitro human cell-based models and assays to accurately identify and validate drug candidates. 3D human cell models can include different tissue cell types and represent the spatiotemporal context of the original tissue (co-cultures), allowing the establishment of biologically-relevant cell-cell and cell-extracellular matrix interactions. Nevertheless, exploitation of these 3D models for neuroprotection assessment has been limited due to the lack of data to validate such 3D co-culture approaches. In this work we combined a 3D human neuron-astrocyte co-culture with a cell viability endpoint for the implementation of a novel in vitro neuroprotection assay, over an oxidative insult. Neuroprotection assay robustness and specificity, and the applicability of Presto Blue, MTT and CytoTox-Glo viability assays to the 3D co-culture were evaluated. Presto Blue was the adequate endpoint as it is non-destructive and is a simpler and reliable assay. Semi-automation of the cell viability endpoint was performed, indicating that the assay setup is amenable to be transferred to automated screening platforms. Finally, the neuroprotection assay setup was applied to a series of 36 test compounds and several candidates with higher neuroprotective effect than the positive control, Idebenone, were identified. The robustness and simplicity of the implemented neuroprotection assay with the cell viability endpoint enables the use of more complex and reliable 3D in vitro cell models to identify and validate drug candidates. Copyright © 2016 Elsevier Inc. All rights reserved.

Heterocyclic Schiff bases as non toxic antioxidants: Solvent effect, structure activity relationship and mechanism of action

Science.gov (United States)

Shanty, Angamaly Antony; Mohanan, Puzhavoorparambil Velayudhan

2018-03-01

Phenolic heterocyclic imine based Schiff bases from Thiophene-2-carboxaldehyde and Pyrrole-2-carboxaldehyde were synthesized and characterized as novel antioxidants. The solvent effects of these Schiff bases were determined and compared with standard antioxidants, BHA employing DPPH assay and ABTS assay. Fixed reaction time and Steady state measurement were used for study. IC50 and EC50 were calculated. Structure-activity relationship revealed that the electron donating group in the phenolic ring increases the activity where as the electron withdrawing moiety decreases the activity. The Schiff base derivatives showed antioxidant property by two different pathways namely SPLET and HAT mechanisms in DPPH assay. While in ABTS method, the reaction between ABTS radical and Schiff bases involves electron transfer followed by proton transfer (ET-PT) mechanism. The cytotoxicity of these compounds has been evaluated by MTT assay. The results showed that all these compounds are non toxic in nature.

Ion sources development at GANIL for radioactive beams and high charge state ions

International Nuclear Information System (INIS)

Leroy, R.; Barue, C.; Canet, C.; Dupuis, M.; Flambard, J.L.; Gaubert, G.; Gibouin, S.; Huguet, Y.; Jardin, P.; Lecesne, N.; Leherissier, P.; Lemagnen, F.; Pacquet, J.Y.; Pellemoine-Landre, F.; Rataud, J.P.; Saint-Laurent, M.G.; Villari, A.C.C.; Maunoury, L.

2001-01-01

The GANIL laboratory has in charge the production of ion beams for nuclear and non nuclear physics. This article reviews the last developments that are underway in the fields of radioactive ion beam production, increase of the metallic ion intensities and production of highly charges ion beams. (authors)

General Atomic's radioactive gas recovery system

International Nuclear Information System (INIS)

Mahn, J.A.; Perry, C.A.

1975-01-01

General Atomic Company has developed a Radioactive Gas Recovery System for the HTGR which separates, for purposes of retention, the radioactive components from the non-radioactive reactor plant waste gases. This provides the capability for reducing to an insignificant level the amount of radioactivity released from the gas waste system to the atmosphere--a most significant improvement in reducing total activity release to the environment. (U.S.)

Membrane Treatment of Liquid Salt Bearing Radioactive Wastes

International Nuclear Information System (INIS)

Dmitriev, S. A.; Adamovich, D. V.; Demkin, V. I.; Timofeev, E. M.

2003-01-01

The main fields of introduction and application of membrane methods for preliminary treatment and processing salt liquid radioactive waste (SLRW) can be nuclear power stations (NPP) and enterprises on atomic submarines (AS) utilization. Unlike the earlier developed technology for the liquid salt bearing radioactive waste decontamination and concentrating this report presents the new enhanced membrane technology for the liquid salt bearing radioactive waste processing based on the state-of-the-art membrane unit design, namely, the filtering units equipped with the metal-ceramic membranes of ''TruMem'' brand, as well as the electrodialysis and electroosmosis concentrators. Application of the above mentioned units in conjunction with the pulse pole changer will allow the marked increase of the radioactive waste concentrating factor and the significant reduction of the waste volume intended for conversion into monolith and disposal. Besides, the application of the electrodialysis units loaded with an ion exchange material at the end polishing stage of the radioactive waste decontamination process will allow the reagent-free radioactive waste treatment that meets the standards set for the release of the decontaminated liquid radioactive waste effluents into the natural reservoirs of fish-farming value

Method of solidifying radioactive laundry wastes

International Nuclear Information System (INIS)

Yasumura, Keijiro

1984-01-01

Purpose: To enable to solidify radioactive laundry wastes containing non-ionic liquid detergents less solidifiable by plastic solidification process in liquid laundry wastes for cloths or the likes discharged from a nuclear power plant. Method: Radioactive laundry wastes are solidified by using plastic solidifying agent comprising, as a main ingredient, unsaturated polyester resins and methylmethacrylate monomers. The plastic solidifying agents usable herein include, for example, unsaturated polyester resins prepared by condensating maleic anhydride and phthalic anhydride with propylene glycol and incorporated with methylmethacrylate monomers. The mixing ratio of the methylmethacrylate monomers is preferably 30 % by weight based on the unsaturated polyester resins. (Aizawa, K.)

Analysis of human blood plasma cell-free DNA fragment size distribution using EvaGreen chemistry based droplet digital PCR assays.

Science.gov (United States)

Fernando, M Rohan; Jiang, Chao; Krzyzanowski, Gary D; Ryan, Wayne L

2018-04-12

Plasma cell-free DNA (cfDNA) fragment size distribution provides important information required for diagnostic assay development. We have developed and optimized droplet digital PCR (ddPCR) assays that quantify short and long DNA fragments. These assays were used to analyze plasma cfDNA fragment size distribution in human blood. Assays were designed to amplify 76,135, 490 and 905 base pair fragments of human β-actin gene. These assays were used for fragment size analysis of plasma cell-free, exosome and apoptotic body DNA obtained from normal and pregnant donors. The relative percentages for 76, 135, 490 and 905 bp fragments from non-pregnant plasma and exosome DNA were 100%, 39%, 18%, 5.6% and 100%, 40%, 18%,3.3%, respectively. The relative percentages for pregnant plasma and exosome DNA were 100%, 34%, 14%, 23%, and 100%, 30%, 12%, 18%, respectively. The relative percentages for non-pregnant plasma pellet (obtained after 2nd centrifugation step) were 100%, 100%, 87% and 83%, respectively. Non-pregnant Plasma cell-free and exosome DNA share a unique fragment distribution pattern which is different from pregnant donor plasma and exosome DNA fragment distribution indicating the effect of physiological status on cfDNA fragment size distribution. Fragment distribution pattern for plasma pellet that includes apoptotic bodies and nuclear DNA was greatly different from plasma cell-free and exosome DNA. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Parallel force assay for protein-protein interactions.

Science.gov (United States)

Aschenbrenner, Daniela; Pippig, Diana A; Klamecka, Kamila; Limmer, Katja; Leonhardt, Heinrich; Gaub, Hermann E

2014-01-01

Quantitative proteome research is greatly promoted by high-resolution parallel format assays. A characterization of protein complexes based on binding forces offers an unparalleled dynamic range and allows for the effective discrimination of non-specific interactions. Here we present a DNA-based Molecular Force Assay to quantify protein-protein interactions, namely the bond between different variants of GFP and GFP-binding nanobodies. We present different strategies to adjust the maximum sensitivity window of the assay by influencing the binding strength of the DNA reference duplexes. The binding of the nanobody Enhancer to the different GFP constructs is compared at high sensitivity of the assay. Whereas the binding strength to wild type and enhanced GFP are equal within experimental error, stronger binding to superfolder GFP is observed. This difference in binding strength is attributed to alterations in the amino acids that form contacts according to the crystal structure of the initial wild type GFP-Enhancer complex. Moreover, we outline the potential for large-scale parallelization of the assay.

Development of a heavy metals enzymatic-based assay using papain

International Nuclear Information System (INIS)

Shukor, Yunus; Baharom, Nor Azlan; Rahman, Fadhil Abd.; Abdullah, Mohd. Puad; Shamaan, Nor Aripin; Syed, Mohd. Arif

2006-01-01

A heavy metals enzymatic-based assay using papain was developed. Papain was assayed using the Casein-coomassie-dye-binding assay. The assay is sensitive to several heavy metals. The IC 50 (concentration of toxicant giving 50% inhibition) of Hg 2+ , Ag 2+ , Pb 2 , Zn 2+ is 0.39, 0.40, 2.16, 2.11 mg l -1 , respectively. For Cu 2+ and Cd 2+ the LOQ (limits of quantitation) is 0.004 and 0.1 mg l -1 , respectively. The IC 50 and LOQ values were found to be generally comparable to several other enzymatic and bioassays tests such as: immobilized urease, 15-min Microtox TM , 48 h Daphnia magna, and 96 h Rainbow trout. The papain assay is xenobiotics tolerant, has a wide pH for optimum activity, is temperature stable, and has a relatively quick assay time. The papain assay was used to identify polluted water samples from industrial sources in Penang, Malaysia. We found one site where the assay gave a positive toxic response. The toxicity of the site was confirmed using Atomic Emission Spectrometry analysis

Radioactive waste disposal

International Nuclear Information System (INIS)

Bohm, H.; Closs, K.D.; Kuhn, K.

1981-01-01

The solutions to the technical problem of the disposal of radioactive waste are limited by a) the state of knowledge of reprocessing possibilites, b) public acceptance of the use of those techniques which are known, c) legislative procedures linking licensing of new nuclear power plants to the solution of waste problems, and d) other political constraints. Wastes are generated in the mining and enriching of radioactive elements, and in the operation of nuclear power plants as well as in all fields where radioactive substances may be used. Waste management will depend on the stability and concentration of radioactive materials which must be stored, and a resolution of the tension between numerous small storage sites and a few large ones, which again face problems of public acceptability

Enhancing usability of augmented-reality-based mobile escape guidelines for radioactive accidents

International Nuclear Information System (INIS)

Tsai, Ming-Kuan; Yau, Nie-Jia

2013-01-01

When radioactive accidents occur, modern tools in information technology for emergency response are good solutions to reduce the impact. Since few information-technology-based applications were developed for people during radioactive accidents, a previous study (Tsai et al., 2012) proposed augmented-reality-based mobile escape guidelines. However, because of the lack of transparent escape routes and indoor escape guidelines, the usability of the guidelines is limited. Therefore, this study introduces route planning and mobile three-dimensional (3D) graphics techniques to address the identified problems. The proposed approach could correctly present the geographical relationship from user locations to the anticipated shelters, and quickly show the floor-plan drawings as users are in the buildings. Based on the testing results, in contrast to the previous study, this study offered better escape routes, when the participants performed self-evacuation in outdoor and indoor environments. Overall, this study is not only a useful reference for similar studies, but also a beneficial tool for emergency response during radioactive accidents. -- Highlights: ► Enhancing the efficiency when people escape from radioactive accidents. ► The spatial relationship is transparently displayed in real time. ► In contrast to a previous study, this study offers better escape guidelines

Quantifying sublethal effects of glyphosate and Roundup® to Daphnia magna using a fluorescence based enzyme activity assay and video tracking

DEFF Research Database (Denmark)

Roslev, Peter; R. Hansen, Lone; Ørsted, Michael

Glyphosate (N-(phosphonomethyl)glycine) is the active ingredient in a range of popular broad-spectrum, non-selective herbicide formulations. The toxicity of this herbicide to non-target aquatic organisms such as Daphnia magna is often evaluated using conventional toxicity assays that focus...... on endpoints such as immobility and mortality. In this study, we investigated sublethal effects of glyphosate and Roundup® to D. magna using video tracking for quantifying behavioral changes, and a novel fluorescence based assay for measuring in vivo hydrolytic enzyme activity (FLEA assay). Roundup® exposure...... resulted in concentration-dependent inhibition of alkaline phosphatase activity in D. magna. The inhibition of alkaline phosphatase by Roundup® was temperature-dependent with lowest inhibition at 14 °C and greater inhibition at 20 and 26 °C. Exposure of D. magna to sublethal concentrations of glyphosate...

Incineration of Non-radioactive Simulated Waste

International Nuclear Information System (INIS)

Ahmed, A.Z.; Abdelrazek, I.D.

1999-01-01

An advanced controlled air incinerator has been investigated, developed and put into successful operation for both non radioactive simulated and other combustible solid wastes. Engineering efforts concentrated on providing an incinerator which emitted a clean, easily treatable off-gas and which produced minimum amounts of secondary waste. Feed material is fed by gravity into the gas reactor without shredding or other pretreatment. The temperature of the waste is gradually increased in a reduced oxygen atmosphere as the resulting products are introduced into the combustion chamber. Steady burning is thus accomplished under easily controlled excess air conditions with the off-gas then passing through a simple dry cleaning-up system. Experimental studies showed that, at lower temperature, CO 2 , and CH 4 contents in gas reactor effluent increase by the increase of glowing bed temperature, while H 2 O, H 2 and CO decrease . It was proved that, a burn-out efficiency (for ash residues) and a volume reduction factor appeared to be better than 95.5% and 98% respectively. Moreover, high temperature permits increased volumes of incinerated material and results in increased gasification products. It was also found that 8% by weight of ashes are separated by flue gas cleaning system as it has chemical and size uniformity. This high incineration efficiency has been obtained through automated control and optimization of process variables like temperature of the glowing bed and the oxygen feed rate to the gas reactor

Radioactive Waste and Clean-up Division

International Nuclear Information System (INIS)

Collard, G.

2001-01-01

The main objectives of the Radioactive Waste and Clean-up division of SCK-CEN are outlined. The division's programme consists of research, development and demonstration projects and aims to contribute to the objectives of Agenda 21 on sustainable development in the field of radioactive waste and rehabilitation of radioactively contaminated sites

Radioactive waste processing

International Nuclear Information System (INIS)

Dejonghe, P.

1978-01-01

This article gives an outline of the present situation, from a Belgian standpoint, in the field of the radioactive wastes processing. It estimates the annual quantity of various radioactive waste produced per 1000 MW(e) PWR installed from the ore mining till reprocessing of irradiated fuels. The methods of treatment concentration, fixation, final storable forms for liquid and solid waste of low activity and for high level activity waste. The storage of radioactive waste and the plutonium-bearing waste treatement are also considered. The estimated quantity of wastes produced for 5450 MW(e) in Belgium and their destination are presented. (A.F.)

Miniaturized Aptamer-Based Assays for Protein Detection

Directory of Open Access Journals (Sweden)

Alessandro Bosco

2016-09-01

Full Text Available The availability of devices for cancer biomarker detection at early stages of the disease is one of the most critical issues in biomedicine. Towards this goal, to increase the assay sensitivity, device miniaturization strategies empowered by the employment of high affinity protein binders constitute a valuable approach. In this work we propose two different surface-based miniaturized platforms for biomarker detection in body fluids: the first platform is an atomic force microscopy (AFM-based nanoarray, where AFM is used to generate functional nanoscale areas and to detect biorecognition through careful topographic measurements; the second platform consists of a miniaturized electrochemical cell to detect biomarkers through electrochemical impedance spectroscopy (EIS analysis. Both devices rely on robust and highly-specific protein binders as aptamers, and were tested for thrombin detection. An active layer of DNA-aptamer conjugates was immobilized via DNA directed immobilization on complementary single-stranded DNA self-assembled monolayers confined on a nano/micro area of a gold surface. Results obtained with these devices were compared with the output of surface plasmon resonance (SPR assays used as reference. We succeeded in capturing antigens in concentrations as low as a few nM. We put forward ideas to push the sensitivity further to the pM range, assuring low biosample volume (μL range assay conditions.

Development of a VHH-Based Erythropoietin Quantification Assay

DEFF Research Database (Denmark)

Kol, Stefan; Beuchert Kallehauge, Thomas; Adema, Simon

2015-01-01

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against...... human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification...

Injectable iodine-125 labeled tissue marker for radioactive localization of non-palpable breast lesions

DEFF Research Database (Denmark)

Schaarup-Jensen, Henrik; Jensen, Andreas Ingemann; Hansen, Anders Elias

2018-01-01

to be spatially well-defined and stable over a seven day period with excellent CT contrast (>1500 HU), enabling fluoroscopic visualization of markers during placement. The radioactivity remains strongly associated with the marker during the implantation period, which limits exposure to healthy tissue....... The surgical guidance marker is based on derivatives of the biomaterial sucrose acetate isobutyrate and unlike currently used markers it is injectable in the tissue using thin needles, reducing the discomfort to the patients significantly. The marker confers CT contrast and has radioactive properties, meaning...

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus.

Science.gov (United States)

Zhu, Yu; Wang, Gui-Hua; Cui, Yu-Dong; Cui, Shang-Jin

2016-09-01

Porcine epidemic diarrhea virus (PEDV) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. Previous studies have indicated that PEDV vaccine strains have a partial deletion in ORF3. In this study, a nanoparticle-assisted polymerase chain reaction (nanoparticle-assisted RT-PCR) assay targeting the ORF3 of PEDV was developed to distinguish PEDV field strains from attenuated strains by using a specific pair of primers. The PCR products of field strains and attenuated strains were 264 bp and 215 bp in length, respectively. The sensitivity and specificity of this assay were also assessed. The nanoparticle-assisted RT-PCR assay was 10-100 times more sensitive than the conventional RT-PCR assay, with no cross-reactions when amplifying porcine pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), classical swine fever virus (CSFV), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine rotavirus (RV), and porcine transmissible gastroenteritis virus (TGEV). The nanoparticle-assisted RT-PCR assay we describe here can be used to distinguish field strains from vaccine strains of PEDV, and it shows promise for reducing economic loss due to PEDV infection.

An optimization of a GPU-based parallel wind field module

International Nuclear Information System (INIS)

Pinheiro, André L.S.; Shirru, Roberto

2017-01-01

Atmospheric radionuclide dispersion systems (ARDS) are important tools to predict the impact of radioactive releases from Nuclear Power Plants and guide people evacuation from affected areas. Four modules comprise ARDS: Source Term, Wind Field, Plume Dispersion and Doses Calculations. The slowest is the Wind Field Module that was previously parallelized using the CUDA C language. The statement purpose of this work is to show the speedup gain with the optimization of the already parallel code of the GPU-based Wind Field module, based in WEST model (Extrapolated from Stability and Terrain). Due to the parallelization done in the wind field module, it was observed that some CUDA processors became idle, thus contributing to a reduction in speedup. It was proposed in this work a way of allocating these idle CUDA processors in order to increase the speedup. An acceleration of about 4 times can be seen in the comparative case study between the regular CUDA code and the optimized CUDA code. These results are quite motivating and point out that even after a parallelization of code, a parallel code optimization should be taken into account. (author)

An optimization of a GPU-based parallel wind field module

Energy Technology Data Exchange (ETDEWEB)

Pinheiro, André L.S.; Shirru, Roberto [Coordenacao de Pos-Graduacao e Pesquisa de Engenharia (PEN/COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear; Pereira, Cláudio M.N.A., E-mail: apinheiro99@gmail.com, E-mail: schirru@lmp.ufrj.br, E-mail: cmnap@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

2017-07-01

Atmospheric radionuclide dispersion systems (ARDS) are important tools to predict the impact of radioactive releases from Nuclear Power Plants and guide people evacuation from affected areas. Four modules comprise ARDS: Source Term, Wind Field, Plume Dispersion and Doses Calculations. The slowest is the Wind Field Module that was previously parallelized using the CUDA C language. The statement purpose of this work is to show the speedup gain with the optimization of the already parallel code of the GPU-based Wind Field module, based in WEST model (Extrapolated from Stability and Terrain). Due to the parallelization done in the wind field module, it was observed that some CUDA processors became idle, thus contributing to a reduction in speedup. It was proposed in this work a way of allocating these idle CUDA processors in order to increase the speedup. An acceleration of about 4 times can be seen in the comparative case study between the regular CUDA code and the optimized CUDA code. These results are quite motivating and point out that even after a parallelization of code, a parallel code optimization should be taken into account. (author)