WorldWideScience

Sample records for non-point source nps

  1. The non-point source (NPS) information system based on remote sensing and GIS and its preliminary application

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A non-point source information system (NPSIS) has been constructed by use of remote sensing and GIS technology, and a construction method of NPSlS introduced with Shenzhen as an example. With the remote sensing land-using images as an environment unit, the analysis modules based on NPSIS are built through combining the NPS model with NPSIS, and the preliminary NPS calculation and analysis performed.

  2. [Zoning planning in non-point source pollution control in Hanyang district].

    Science.gov (United States)

    Yang, Liu; Ma, Ke-Ming; Guo, Qing-Hai; Zhao, Jing-Zhu; Luo, Yong-Feng

    2006-01-01

    It is most important for managing urban non-point source (NPS) pollution, actualizing the urban sustainable development as well, that zoning planning of urban NPS pollution control is studied. A case study on principles and methods of zoning planning in urban NPS pollution is carried out. Principles of urban sustainable development, priority of urban NPS pollution sensitivity, similarity of urban NPS control direction and region conjugate are put forward. Besides, it is for the first time that a more quantitive method is presented, in the case of Hanyang district, Wuhan city, which is based on L-THIA model and spatial analysis technique in GIS. Assessment of NPS pollution status quo, as well as analysis of NPS sensitivity, is the kernel component of the quantitive method. Hanyang might be divided into four NPS pollution control zones. It is helpful for decision-making of regional NPS pollution control.

  3. [Impacts of the urbanization on waters non-point source pollution].

    Science.gov (United States)

    Yang, Liu; Ma, Ke-Ming; Guo, Qing-hai; Zhao, Jing-zhu

    2004-11-01

    Non-point source (NPS) pollution is the prominent source of water pollution in many countries, included America and China, of the world. Urban NPS pollution was attached little importance for long, compared with agriculture NPS pollution. While urbanization is the dominant form of land-use change in terms of impacts on water quality, the hydrology, other physical properties of watersheds as well as their NPS pollution potential at present. The formation of urban NPS pollution of water could be described by "source-process-sink". Urbanization has changed the source, process and sink of urban NPS pollution. A review was conducted on the international researches of urbanization impacts on NPS pollution in urban water environment from the point of view of "describe-predict and evaluation-application". The studies of urbanization impacts on urban NPS pollution were focused on modeling the process of urban NPS pollution by hydrologic model, predicting the pollutants load of NPS pollution. It is a fresh methodology that the relationship between urbanization and urban NPS pollution of water was analyzed by the method of landscape change and ecological process. The research on temporal-spatial comprehensive impacts of landscape pattern changes, led by urbanization, on the urban NPS pollution will be one of the hotspots.

  4. Long-term vegetation landscape pattern with non-point source nutrient pollution in upper stream of Yellow River basin

    NARCIS (Netherlands)

    Ouyang, W.; Skidmore, A.K.; Toxopeus, A.G.; Hao, F.

    2010-01-01

    Grassland, forest, and farmland are the dominant land covers in upper catchments of the Yellow River and their landscape status has direct connection with dynamics of non-point source (NPS) pollution. Understanding the correlations between landscape variables and different formats of NPS nutrients p

  5. Long-term vegetation landscape pattern with non-point source nutrient pollution in upper stream of Yellow River basin

    NARCIS (Netherlands)

    Ouyang, W.; Skidmore, A.K.; Toxopeus, A.G.; Hao, F.

    2010-01-01

    Grassland, forest, and farmland are the dominant land covers in upper catchments of the Yellow River and their landscape status has direct connection with dynamics of non-point source (NPS) pollution. Understanding the correlations between landscape variables and different formats of NPS nutrients p

  6. Relationship Between Non-Point Source Pollution and Korean Green Factor

    OpenAIRE

    Seung Chul Lee; In-Hyeok Park; Byung Sik Kim; and Sung Ryong Ha

    2015-01-01

    In determining the relationship between the rational event mean concentration (REMC) which is a volume-weighted mean of event mean concentrations (EMCs) as a non-point source (NPS) pollution indicator and the green factor (GF) as a low impact development (LID) land use planning indicator, we constructed at runoff database containing 1483 rainfall events collected from 107 different experimental catchments from 19 references in Korea. The collected data showed that EMCs were not correlated wit...

  7. Mapping the scientific research on non-point source pollution: a bibliometric analysis.

    Science.gov (United States)

    Yang, Beibei; Huang, Kai; Sun, Dezhi; Zhang, Yue

    2017-02-01

    A bibliometric analysis was conducted to examine the progress and future research trends of non-point source (NPS) pollution during the years 1991-2015 based on the Science Citation Index Expanded (SCI-Expanded) of Web of Science (WoS). The publications referencing NPS pollution were analyzed including the following aspects: document type, publication language, publication output and characteristics, subject category, source journal, distribution of country and institution, author keywords, etc. The results indicate that the study of NPS pollution demonstrated a sharply increasing trend since 1991. Article and English were the most commonly used document type and language. Environmental sciences and ecology, water resources, and engineering were the top three subject categories. Water science and technology ranked first in distribution of journal, followed by Science of the total environment and Environmental Monitoring and Assessment. The USA took a leading position in both quantity and quality, playing an important role in the research field of NPS pollution, followed by the UK and China. The most productive institution was the Chinese Academy of Sciences (Chinese Acad Sci), followed by Beijing Normal University and US Department of Agriculture's Agricultural Research Service (USDA ARS). The analysis of author keywords indicates that the major hotspots of NPS pollution from 1991 to 2015 contained "water," "model," "agriculture," "nitrogen," "phosphorus," etc. The results provide a comprehensive understanding of NPS pollution research and help readers to establish the future research directions.

  8. Controlling Non-Point Source Pollution in Australian Agricultural Systems

    Institute of Scientific and Technical Information of China (English)

    C. GOURLEY; A. RIDLEY

    2005-01-01

    The Australian farming sector is continuing to intensify, particularly within 300 km of the east and southern coastlines.In the future there will be fewer and larger farms, which will use more fertilizer, support more stock, grow more monoculture crops, and utilise more marginal soils. This is likely to increase the major environmental impacts of soil degradation, salt,nutrient and sediment contamination of waterways, and greenhouse gas emissions. Australian national water policy continues to focus on land, stream and groundwater salinity issues, although there is now a greater recognition of the importance of nitrogen and phosphorus losses from agriculture. The general philosophy of policy for dealing with nonpoint source pollution has been towards a voluntary rather than regulatory approach, with state and national governments supporting a range of programs to encourage sustainable agricultural practices. A catchment (watershed) based approach,through the use of integrated catchment management plans, is the primary way that non-point source pollution is addressed at the farm and local level. At an industry level, cotton, grains, meat, sugarcane and dairy amongst others, as well as the Australian fertilizer industry, have responded to non-point source issues by investing in research and development, and developing codes of practice aimed at abating these environmental impacts. Understanding the economic, social, political and cultural contexts of farming as well as the environmental impacts of agriculture are very important in determining the appropriateness of policy responses for Australian farming systems.

  9. Loss coefficient of nitrogenous non-point source pollution under various precipitation conditions

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In this study,calibrations of non-point source (NPS) pollution models are performed based on Black River basin historical real-time runoff data,sedimentation record data,and NPS sources survey information.The concept of NPS loss coefficient for the watershed or the loss coefficients (LC) for simplicity is brought up by examining NPS build-up and migration processes along riverbanks in natural river systems.The historical data is used for determining the nitrogenous NPS loss coefficient for five land use types including farmland,urban land,grassland,shrub land,and forest under different precipitation conditions.The comparison of outputs from Soil and Water Assessment Tool (SWAT) model and coefficient export method showed that both methods could obtain reasonable LC.The high Pearson correlation coefficient (0.94722) between those two sets of calculation results justified the consistency of those two models.Another result in the study is that different combinations of precipitation condition and land use types could significantly affect the calculated loss coefficient.As for the adsorptive nitrogen,the order of impact on LC for different land use types can be sorted as:farm land > urban land > grassland > shrub land > forest while the order was farmland > grass land > shrub land > forest > urban land for soluble nitrogen.

  10. Reducing non-point source pollution with enhancing infiltration

    Institute of Scientific and Technical Information of China (English)

    MU Huan-zhen; ZHENG Tao; HUANG Yan-chu; ZHANG Chun-ping; LIU Chen

    2006-01-01

    The rainfall system was set up on a slope land, which was used with some materials to enhance soil infiltration. The results showed that it was effective to enhance the infiltration of rainwater in soil and reduce the pollutants of surface runoff. After the soil meliorated by the lignin polymer and zeolite, runoff was delayed about 10 min and reduced by 44.40%-50.00%, synchronously, the pollutant loads, such as total suspended solids (TSS), chemical oxygen demand by ditromate (CODCr), total nitroger (TN) and total phosphorus (TP), were reduced on averages by 44.58%, 37.80%, 51.62% and 44.11%, respectively. It is an available technique to control the pollution of non-point source from sources.

  11. Application of a constructed wetland for non-point source pollution control.

    Science.gov (United States)

    Kao, C M; Wang, J Y; Lee, H Y; Wen, C K

    2001-01-01

    In Taiwan, non-point source (NPS) pollution is one of the major causes of impairment of surface waters. The main objective of this study was to evaluate the efficacy of using constructed wetlands on NPS pollutant removal and water quality improvements. A field-scale constructed wetland system was built inside the campus of National Sun Yat-Sen University (located in southern Taiwan) to remove (1) NPS pollutants due to the stormwater runoff, and (2) part of the untreated wastewater from school drains. The constructed wetland was 40 m (L) x 30 m (W) x 1 m (D), which received approximately 85 m3 per day of untreated wastewater from school drainage pipes. The plants grown on the wetland included floating (Pistia stratiotes L.) and emergent (Phragmites communis L.) species. One major storm event and baseline water quality samples were analyzed during the monitoring period. Analytical results indicate that the constructed wetland removed a significant amount of NPS pollutants and wastewater constituents. More than 88% of nitrogen, 81% of chemical oxygen demand (COD), 85% of heavy metals, and 60% of the total suspended solids (TSS) caused by the storm runoff were removed by the wetland system before discharging. Results from this study may be applied to the design of constructed wetlands for NPS pollution control and water quality improvement.

  12. Introduction: Assessing non-point source pollution in the vadose zone with advanced information technologies

    Science.gov (United States)

    Corwin, Dennis L.; Loague, Keith; Ellsworth, Timothy R.

    The information age has ushered in a global awareness of complex environmental problems that do not respect political or physical boundaries: climatic change, ozone layer depletion, deforestation, desertification, and non-point source (NPS) pollution. Among these global environmental problems, NPS pollutants represent a perfect example of a complex multidisciplinary problem that exists over multiple scales with tremendous spatial and temporal complexity. To address the NPS problem, specific to the vadose zone, advanced information technologies must be applied in a spatial context. An integrated system of advanced information technologies (i.e., global positioning, geographic information system, geostatistics, remote sensing, solute transport modeling, neural networks, transfer functions, fuzzy logic, hierarchical theory, and uncertainty analysis) provides a framework from which real-time and/or simulated assessments of NPS pollution can be made. The ability to accurately assess present and future NPS-pollution impacts on ecosystems ranging from local to global scales provides a powerful tool for environmental stewardship and guiding future human activities.

  13. [L-THIA-based management design for controlling urban non-point source pollution].

    Science.gov (United States)

    Guo, Qing-Hai; Yang, Liu; Ke-Ming, Ma

    2007-11-01

    L-THIA Model was used to simulate the amounts of NPS pollutants in 2 catchments of Sanjiao watershed (Sj1, Sj2) in Hanyang district, and the total simulated amount of NPS loads in Sj1 and Sj2 were 1.82 x 10(4) kg, 1.38 x 10(5) kg, respectively. Based on the theory of resource-sink" and interaction of pattern with process, a series of BMPs, including green roof, grassland, porous pavement, infiltration trench, vegetative filter strip and wet pond, were optimized, and effects of BMPs were simulated along the surface runoff pathway. The results show that total pollutants outputs entering Sj1 and Sj2 account for 14.65% and 6.57%, respectively. Combining L-THIA model and BMPs in series is a proper measure for non-point source pollution control and urban development planning at watershed or region scale.

  14. Parameter uncertainty analysis of non-point source pollution from different land use types.

    Science.gov (United States)

    Shen, Zhen-yao; Hong, Qian; Yu, Hong; Niu, Jun-feng

    2010-03-15

    Land use type is one of the most important factors that affect the uncertainty in non-point source (NPS) pollution simulation. In this study, seventeen sensitive parameters were screened from the Soil and Water Assessment Tool (SWAT) model for parameter uncertainty analysis for different land use types in the Daning River Watershed of the Three Gorges Reservoir area, China. First-Order Error Analysis (FOEA) method was adopted to analyze the effect of parameter uncertainty on model outputs under three types of land use, namely, plantation, forest and grassland. The model outputs selected in this study consisted of runoff, sediment yield, organic nitrogen (N), and total phosphorus (TP). The results indicated that the uncertainty conferred by the parameters differed among the three land use types. In forest and grassland, the parameter uncertainty in NPS pollution was primarily associated with runoff processes, but in plantation, the main uncertain parameters were related to runoff process and soil properties. Taken together, the study suggested that adjusting the structure of land use and controlling fertilizer use are helpful methods to control the NPS pollution in the Daning River Watershed.

  15. Regional-scale assessment of non-point source groundwater contamination

    Science.gov (United States)

    Loague, Keith; Corwin, Dennis L.

    1998-05-01

    Predictive assessments of non-point source (NPS) pollution can have great utility for environmentally focused land use decisions related to both the remediation of existing groundwater contamination and the regulation of current (and future) agrochemical use. At the regional scales associated with NPS agrochemical applications there are staggering data management problems in assessing potential groundwater vulnerability. Geographical information system (GIS) technology is a timely tool that greatly facilitates the organized characterization of regional-scale variability. In this paper we review the recently reported (Loague et al., 1998a,b) simulations of NPS groundwater vulnerability, resulting from historical applications of the agrochemical DBCP (1,2-dibromo-3-chloropropane), for east-central Fresno County (California). The Fresno case study helps to illustrate the data requirements associated with process-based three-dimensional simulations of coupled fluid flow and solute transport in the unsaturated/saturated subsurface at a regional scale. The strengths and weaknesses of using GIS in regional-scale vulnerability assessments, such as the Fresno case study, and the critical problem of estimating the uncertainties in these assessments (owing to both data and model errors) are discussed. A regional GIS-driven integrated assessment approach is proposed, which is based upon cost-benefit analysis, and incorporates both physical and economic factors that can be used in a regulatory decision process.

  16. The Roles of Countrywomen in Controlling Non-point Source Pollution

    Institute of Scientific and Technical Information of China (English)

    Jiang Dongmei; Zhou Yuanfang; Lu Genfa

    2006-01-01

    The main causes of non-point source pollution in Taihu Lake are the improper ways of crop production, animal husbandry, and daily runoff. The paper discusses the relationship between countrywomen and non-point source pollution control by 731 questionnaires in Weidu village and 466 questionnaires in Dapu Town. The roles of countrywomen have changed in families and they have close relationship with non-point source pollution. Furthermore, we discuss the possibility and methods of organizing countrywomen in non-point source pollution control.

  17. Long-term agricultural non-point source pollution loading dynamics and correlation with outlet sediment geochemistry

    Science.gov (United States)

    Ouyang, Wei; Jiao, Wei; Li, Xiaoming; Giubilato, Elisa; Critto, Andrea

    2016-09-01

    Some agricultural non-point source (NPS) pollutants accumulate in sediments in the outlet sections of watersheds. It is crucial to evaluate the historical interactions between sediment properties and watershed NPS loading. Therefore, a sediment core from the outlet of an agricultural watershed was collected. The core age was dated using the 210Pb method, and sedimentation rates were determined using the constant rate of supply (CRS) model. The total nitrogen (TN), total phosphorus (TP), Cd, Pb, Cu, Ni and Cr accumulations in the sediment generally showed fluctuating increases, with the highest sedimentation fluxes all occurring in approximately 1998. The measurement of specific mass sedimentation rates reflected a record of watershed soil erosion dynamics. Using SWAT (Soil and Water Assessment Tool) to simulate long-term watershed agricultural NPS pollution loadings, the historical interactions between sediment properties and NPS loadings were further evaluated. The N leaching process weakened these interactions, but the historical accumulations of TP and heavy metals in sediments generally correlated well with watershed NPS TP loading. The regression analysis suggested that Pb and Cr were the most suitable indexes for assessing long-term NPS TN and TP pollution, respectively. Assessing the NPS loading dynamics using the vertical characteristics of sediment geochemistry is a new method.

  18. [A landscape ecological approach for urban non-point source pollution control].

    Science.gov (United States)

    Guo, Qinghai; Ma, Keming; Zhao, Jingzhu; Yang, Liu; Yin, Chengqing

    2005-05-01

    Urban non-point source pollution is a new problem appeared with the speeding development of urbanization. The particularity of urban land use and the increase of impervious surface area make urban non-point source pollution differ from agricultural non-point source pollution, and more difficult to control. Best Management Practices (BMPs) are the effective practices commonly applied in controlling urban non-point source pollution, mainly adopting local repairing practices to control the pollutants in surface runoff. Because of the close relationship between urban land use patterns and non-point source pollution, it would be rational to combine the landscape ecological planning with local BMPs to control the urban non-point source pollution, which needs, firstly, analyzing and evaluating the influence of landscape structure on water-bodies, pollution sources and pollutant removal processes to define the relationships between landscape spatial pattern and non-point source pollution and to decide the key polluted fields, and secondly, adjusting inherent landscape structures or/and joining new landscape factors to form new landscape pattern, and combining landscape planning and management through applying BMPs into planning to improve urban landscape heterogeneity and to control urban non-point source pollution.

  19. Relationship Between Non-Point Source Pollution and Korean Green Factor

    Directory of Open Access Journals (Sweden)

    Seung Chul Lee

    2015-01-01

    Full Text Available In determining the relationship between the rational event mean concentration (REMC which is a volume-weighted mean of event mean concentrations (EMCs as a non-point source (NPS pollution indicator and the green factor (GF as a low impact development (LID land use planning indicator, we constructed at runoff database containing 1483 rainfall events collected from 107 different experimental catchments from 19 references in Korea. The collected data showed that EMCs were not correlated with storm factors whereas they showed significant differences according to the land use types. The calculated REMCs for BOD, COD, TSS, TN, and TP showed negative correlations with the GFs. However, even though the GFs of the agricultural area were concentrated in values of 80 like the green areas, the REMCs for TSS, TN, and TP were especially high. There were few differences in REMC runoff characteristics according to the GFs such as recreational facilities areas in suburbs and highways and trunk roads that connect to major roads between major cities. Except for those areas, the REMCs for BOD and COD were significantly related to the GFs. The REMCs for BOD and COD decreased when the rate of natural green area increased. On the other hand, some of the REMCs for TSS, TN, and TP were still high where the catchments encountered mixed land use patterns, especially public facility areas with bare ground and artificial grassland areas. The GF could therefore be used as a major planning indicator when establishing land use planning aimed at sustainable development with NPS management in urban areas if the weighted GF values will be improved.

  20. Assessment of Economic Loss Caused by Agricultural Non-point Source Nutrient Loss

    Institute of Scientific and Technical Information of China (English)

    FAN Liang-qian; CHEN Feng-hui

    2012-01-01

    Taking Zhejiang Province as an example, we use the JOHNES export coefficient model to estimate the total nitrogen (TN) and total phosphorus (TP) load of agricultural planting, livestock and poultry breeding and rural living non-point source in 2009. Based on the protection cost method in environmental economics, we quantitatively assess the economic loss caused by these three types of non-point source nutrient loss. The results show that in TN non-point source load, the load of land for planting accounts for 57.48%, the load of rural living accounts for 30.22%, and the load of livestock and poultry breeding accounts for 12.30%; in TP non-point source load, the load of rural living accounts for 46.18%, the load of livestock and poultry breeding accounts for 29.00%, and the load of land for planting accounts for 24.82%. The economic loss arising from the agricultural non-point source nutrient loss is equivalent to 2.329 424 7 billion yuan per year (the loss from land for planting accounts for 55.46%; the loss from rural living accounts for 31.21%; the loss from livestock and poultry breeding accounts for 13.33%). It indicates that in order to reduce the loss arising from agricultural non-point source nutrient loss, we should pay attention to controlling the land for planting and rural living source.

  1. Landscape planning for agricultural non-point source pollution reduction. II. Balancing watershed size, number of watersheds, and implementation effort.

    Science.gov (United States)

    Maxted, Jeffrey T; Diebel, Matthew W; Vander Zanden, M Jake

    2009-01-01

    Agricultural non-point source (NPS) pollution poses a severe threat to water quality and aquatic ecosystems. In response, tremendous efforts have been directed toward reducing these pollution inputs by implementing agricultural conservation practices. Although conservation practices reduce pollution inputs from individual fields, scaling pollution control benefits up to the watershed level (i.e., improvements in stream water quality) has been a difficult challenge. This difficulty highlights the need for NPS reduction programs that focus efforts within target watersheds and at specific locations within target watersheds, with the ultimate goal of improving stream water quality. Fundamental program design features for NPS control programs--i.e., number of watersheds in the program, total watershed area, and level of effort expended within watersheds--have not been considered in any sort of formal analysis. Here, we present an optimization model that explores the programmatic and environmental trade-offs between these design choices. Across a series of annual program budgets ranging from $2 to $200 million, the optimal number of watersheds ranged from 3 to 27; optimal watershed area ranged from 29 to 214 km(2); and optimal expenditure ranged from $21,000 to $35,000/km(2). The optimal program configuration was highly dependent on total program budget. Based on our general findings, we delineated hydrologically complete and spatially independent watersheds ranging in area from 20 to 100 km(2). These watersheds are designed to serve as implementation units for a targeted NPS pollution control program currently being developed in Wisconsin.

  2. Assessment of the relationship between rural non-point source pollution and economic development in the Three Gorges Reservoir Area.

    Science.gov (United States)

    Zhang, Tong; Ni, Jiupai; Xie, Deti

    2016-04-01

    This study investigates the relationship between rural non-point source (NPS) pollution and economic development in the Three Gorges Reservoir Area (TGRA) by using the Environmental Kuznets Curve (EKC) hypothesis for the first time. Five types of pollution indicators, namely, fertilizer input density (FD), pesticide input density (PD), agricultural film input density (AD), grain residues impact (GI), and livestock manure impact (MI), were selected as rural NPS pollutant variables. Rural net income per capita was used as the indicator of economic development. Pollution load was generated by agricultural inputs (consumption of fertilizer, pesticide, and agricultural film) and economic growth with invert U-shaped features. The predicted turning points for FD, PD, and AD were at rural net income per capita levels of 6167.64, 6205.02, and 4955.29 CNY, respectively, which were all surpassed. However, the features between agricultural waste outputs (grain residues and livestock manure) and economic growth were inconsistent with the EKC hypothesis, which reflected the current trends of agricultural economic structure in the TGRA. Given that several other factors aside from economic development level could influence the pollutant generation in rural NPS, a further examination with long-run data support should be performed to understand the relationship between rural NPS pollution and income level.

  3. Evaluation of the Agricultural Non-point Source Pollution in Chongqing Based on PSR Model

    Institute of Scientific and Technical Information of China (English)

    Hanwen; ZHANG; Xinli; MOU; Hui; XIE; Hong; LU; Xingyun; YAN

    2014-01-01

    Through a series of exploration based on PSR framework model,for the purpose of building a suitable Chongqing agricultural nonpoint source pollution evaluation index system model framework,combined with the presence of Chongqing specific agro-environmental issues,we build a agricultural non-point source pollution assessment index system,and then study the agricultural system pressure,agro-environmental status and human response in total 3 major categories,develope an agricultural non-point source pollution evaluation index consisting of 3 criteria indicators and 19 indicators. As can be seen from the analysis,pressures and responses tend to increase and decrease linearly,state and complex have large fluctuations,and their fluctuations are similar mainly due to the elimination of pressures and impact,increasing the impact for agricultural non-point source pollution.

  4. Game Analysis and Strategy Research of Farmers Involving in the Agricultural Non-Point Source Pollution Prevention and Control

    OpenAIRE

    Zhou Zaohong

    2013-01-01

    This thesis applies game theory to make a quantitative analysis of the outward problem of the agricultural non-point source pollution and discusses the basic starting point to study the agricultural non-point source pollution prevention and control and furthermore researches the measures and recommendations of agricultural non-point source pollution prevention and control.

  5. Game Analysis and Strategy Research of Farmers Involving in the Agricultural Non-Point Source Pollution Prevention and Control

    Directory of Open Access Journals (Sweden)

    Zhou Zaohong

    2013-06-01

    Full Text Available This thesis applies game theory to make a quantitative analysis of the outward problem of the agricultural non-point source pollution and discusses the basic starting point to study the agricultural non-point source pollution prevention and control and furthermore researches the measures and recommendations of agricultural non-point source pollution prevention and control.

  6. Impacts of DEM uncertainties on critical source areas identification for non-point source pollution control based on SWAT model

    Science.gov (United States)

    Xu, Fei; Dong, Guangxia; Wang, Qingrui; Liu, Lumeng; Yu, Wenwen; Men, Cong; Liu, Ruimin

    2016-09-01

    The impacts of different digital elevation model (DEM) resolutions, sources and resampling techniques on nutrient simulations using the Soil and Water Assessment Tool (SWAT) model have not been well studied. The objective of this study was to evaluate the sensitivities of DEM resolutions (from 30 m to 1000 m), sources (ASTER GDEM2, SRTM and Topo-DEM) and resampling techniques (nearest neighbor, bilinear interpolation, cubic convolution and majority) to identification of non-point source (NPS) critical source area (CSA) based on nutrient loads using the SWAT model. The Xiangxi River, one of the main tributaries of Three Gorges Reservoir in China, was selected as the study area. The following findings were obtained: (1) Elevation and slope extracted from the DEMs were more sensitive to DEM resolution changes. Compared with the results of the 30 m DEM, 1000 m DEM underestimated the elevation and slope by 104 m and 41.57°, respectively; (2) The numbers of subwatersheds and hydrologic response units (HRUs) were considerably influenced by DEM resolutions, but the total nitrogen (TN) and total phosphorus (TP) loads of each subwatershed showed higher correlations with different DEM sources; (3) DEM resolutions and sources had larger effects on CSAs identifications, while TN and TP CSAs showed different response to DEM uncertainties. TN CSAs were more sensitive to resolution changes, exhibiting six distribution patterns at all DEM resolutions. TP CSAs were sensitive to source and resampling technique changes, exhibiting three distribution patterns for DEM sources and two distribution patterns for DEM resampling techniques. DEM resolutions and sources are the two most sensitive SWAT model DEM parameters that must be considered when nutrient CSAs are identified.

  7. mpacts of Agricultural Non-point Pollution on Water-source Area in Songhua Dam

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The aim was to research impacts of agricultural non-point pol- lution on water-source region in Songhua Dam, laying foundation for control of water pollution and scientific protection of water-source region. [Method] Water in Muyang River, lengshui River and Zizania aquatica region were sampled to measure content of pollutants in water and conclude relation between water contamination and agri- cultural non-point pollution to find the major cause of pollution. [Result] Organic pollu- tant in Muyang River was higher; N and P contents in Lengshui River were higher; the measured indices in Zizania aquatica region excessively exceeded related stan- dard. [Conclusion] The chemical fertilizers and pesticides are the toxic materials lead- ing to water contamination and constitute a major cause of pollution in Songhua Dam water-source region. Agricultural non-point pollution should be controlled in a scientific way.

  8. Assessing the effects of rural livelihood transition on non-point source pollution: a coupled ABM-IECM model.

    Science.gov (United States)

    Yuan, Chengcheng; Liu, Liming; Ye, Jinwei; Ren, Guoping; Zhuo, Dong; Qi, Xiaoxing

    2017-04-02

    Water pollution caused by anthropogenic activities and driven by changes in rural livelihood strategies in an agricultural system has received increasing attention in recent decades. To simulate the effects of rural household livelihood transition on non-point source (NPS) pollution, a model combining an agent-based model (ABM) and an improved export coefficient model (IECM) was developed. The ABM was adopted to simulate the dynamic process of household livelihood transition, and the IECM was employed to estimate the effects of household livelihood transition on NPS pollution. The coupled model was tested in a small catchment in the Dongting Lake region, China. The simulated results reveal that the transition of household livelihood strategies occurred with the changes in the prices of rice, pig, and labor. Thus, the cropping system, land-use intensity, resident population, and number of pigs changed in the small catchment from 2000 to 2014. As a result of these changes, the total nitrogen load discharged into the river initially increased from 6841.0 kg in 2000 to 8446.3 kg in 2004 and then decreased to 6063.9 kg in 2014. Results also suggest that rural living, livestock, paddy field, and precipitation alternately became the main causes of NPS pollution in the small catchment, and the midstream region of the small catchment was the primary area for NPS pollution from 2000 to 2014. Despite some limitations, the coupled model provides an innovative way to simulate the effects of rural household livelihood transition on NPS pollution with the change of socioeconomic factors, and thereby identify the key factors influencing water pollution to provide valuable suggestions on how agricultural environmental risks can be reduced through the regulation of the behaviors of farming households in the future.

  9. Estimation of Non-point Source Pollution Loads Under Uncertain Information

    Institute of Scientific and Technical Information of China (English)

    LI Ruzhong

    2008-01-01

    Many kinds of uncertainties are involved, such as random, fuzzy, grey, unascertained property and so on, in soil erosion process. To exactly predict the non-point source pollution loads, some uncertainties should be taken into consideration. Aiming at the deficiency of present blind number theory being helpless for fuzziness, a novel blind number, i.e. extended-blind number, was introduced by substituting a set of triangular fuzzy numbers (TFNs), ex-pressed as α-cuts, for interval values in present blind number, and the expected value of extended-blind number was also brought forward by referring to the current blind number theory. On the basis of denoting the parameters of Uni-versal Soil Loss Equation (USLE) as extended-blind parameters, a novel USLE model was established for quantita-tively evaluating soil erosion loss and non-point source pollution loads. As a case, the uncertain USLE was employed for predicting the soil erosion loss and non-point source pollution loads of absorbed nitrogen and phosphorus in a dis-trict in the Hangbu-Fengle River basin, in the upstream of Chaohu Lake watershed. The results show that it is feasible in theory to extend blind number into fuzzy environment and reliable on conclusion to apply extended-blind number theory for predicting non-point source pollution loads.

  10. Another Look at the Income Elasticity of Non-point Source Air Pollutants: A Semiparametric Approach

    NARCIS (Netherlands)

    Roy, N.; Kooten, van G.C.

    2004-01-01

    In this paper, a semiparametric model is used to examine the relationship between pollution and income for three non-point source pollutants. Statistical tests reject the quadratic specification in favor of the semiparametric model in all cases. However, the results do not support the inverted-U hyp

  11. Distributed Simulation of Non-Point Source Pollution in Ashi River Basin

    Institute of Scientific and Technical Information of China (English)

    Fang Ma; Xiaofeng Jiang; Li Wang; Zhe Li; Xiongwei Liang

    2015-01-01

    In order to get a thorough understanding of non⁃point source pollution, it is essential to examine its temporal and spatial distribution. A physically⁃based distributed model, Soil and Water Assessment Tool ( SWAT) , was used in this research, to quantitatively estimate the NPS load and analyze the temporal and spatial distributions of NPS pollution in Ashi River Basin. The results indicated that SWAT was suitable to simulate stream⁃flow and water quality in Ashi River Basin. Total Nitrogen which was contributed by NPS (NPS⁃TN) accounted for 32�47%-62�61%, and Total Phosphorus which was contributed by NPS (NPS⁃TP) accounted for 22�30%-57�85% of the total load respectively. In inter⁃annual timescale, both NPS⁃TN and NPS⁃TP were influenced by stream⁃flow and fertilizer. However, when compared with fertilizer, NPS pollution was more directly affected by stream⁃flow. In annual timescale, NPS⁃TN and NPS⁃TP mainly occurred in flood season (from May to September). In the aspect of space, spatial differences of NPS⁃TN and NPS⁃TP were extremely significant. The spatial variations of NPS pollution were mainly influenced by land use, precipitation, soil and slope.

  12. THE METHOD OF CAR HEADLIGHTS LUMINOUS INTENSITY MEASURING FOR NON-POINT SOURCES OF LIGHT

    Directory of Open Access Journals (Sweden)

    A. Kupko

    2015-12-01

    Full Text Available It is shown that the measurements of luminous intensity of car headlights luminous intensity for non-point sources have pecularities. A simplified method for correction the luminous intensity at various distances is developed. The applicability of the given method with possible measurement errors is studied. The results were obtained, using a stand of the National Scientific Center “Institute of Metroligy”.

  13. Response of non-point source pollutant loads to climate change in the Shitoukoumen reservoir catchment.

    Science.gov (United States)

    Zhang, Lei; Lu, Wenxi; An, Yonglei; Li, Di; Gong, Lei

    2012-01-01

    The impacts of climate change on streamflow and non-point source pollutant loads in the Shitoukoumen reservoir catchment are predicted by combining a general circulation model (HadCM3) with the Soil and Water Assessment Tool (SWAT) hydrological model. A statistical downscaling model was used to generate future local scenarios of meteorological variables such as temperature and precipitation. Then, the downscaled meteorological variables were used as input to the SWAT hydrological model calibrated and validated with observations, and the corresponding changes of future streamflow and non-point source pollutant loads in Shitoukoumen reservoir catchment were simulated and analyzed. Results show that daily temperature increases in three future periods (2010-2039, 2040-2069, and 2070-2099) relative to a baseline of 1961-1990, and the rate of increase is 0.63°C per decade. Annual precipitation also shows an apparent increase of 11 mm per decade. The calibration and validation results showed that the SWAT model was able to simulate well the streamflow and non-point source pollutant loads, with a coefficient of determination of 0.7 and a Nash-Sutcliffe efficiency of about 0.7 for both the calibration and validation periods. The future climate change has a significant impact on streamflow and non-point source pollutant loads. The annual streamflow shows a fluctuating upward trend from 2010 to 2099, with an increase rate of 1.1 m(3) s(-1) per decade, and a significant upward trend in summer, with an increase rate of 1.32 m(3) s(-1) per decade. The increase in summer contributes the most to the increase of annual load compared with other seasons. The annual NH (4) (+) -N load into Shitoukoumen reservoir shows a significant downward trend with a decrease rate of 40.6 t per decade. The annual TP load shows an insignificant increasing trend, and its change rate is 3.77 t per decade. The results of this analysis provide a scientific basis for effective support of decision

  14. Uncertainty Analysis of non-point source pollution control facilities design techniques in Korea

    Science.gov (United States)

    Lee, J.; Okjeong, L.; Gyeong, C. B.; Park, M. W.; Kim, S.

    2015-12-01

    The design of non-point sources control facilities in Korea is divided largely by the stormwater capture ratio, the stormwater load capture ratio, and the pollutant reduction efficiency of the facility. The stormwater capture ratio is given by a design formula as a function of the water quality treatment capacity, the greater the capacity, the more the amount of stormwater intercepted by the facility. The stormwater load capture ratio is defined as the ratio of the load entering the facility of the total pollutant load generated in the target catchment, and is given as a design formula represented by a function of the stormwater capture ratio. In order to estimate the stormwater capture ratio and load capture ratio, a lot of quantitative analysis of hydrologic processes acted in pollutant emission is required, but these formulas have been applied without any verification. Since systematic monitoring programs were insufficient, verification of these formulas was fundamentally impossible. However, recently the Korean ministry of Environment has conducted an long-term systematic monitoring project, and thus the verification of the formulas became possible. In this presentation, the stormwater capture ratio and load capture ratio are re-estimated using actual TP data obtained from long-term monitoring program at Noksan industrial complex located in Busan, Korea. Through the re-estimated process, the uncertainty included in the design process that has been applied until now will be shown in a quantitative extent. In addition, each uncertainty included in the stormwater capture ratio estimation and in the stormwater load capture ratio estimation will be expressed to quantify the relative impact on the overall non-point pollutant control facilities design process. Finally, the SWMM-Matlab interlocking module for model parameters estimation will be introduced. Acknowledgement This subject is supported by Korea Ministry of Environment as "The Eco Innovation Project : Non-point

  15. Spatial-temporal characteristics of phosphorus in non-point source pollution with grid-based export coefficient model and geographical information system.

    Science.gov (United States)

    Liu, Ruimin; Dong, Guangxia; Xu, Fei; Wang, Xiujuan; He, Mengchang

    2015-01-01

    In this paper, the spatial changes and trends in non-point source (NPS) total phosphorus (TP) pollution were analyzed by land and non-land uses in the Songliao River Basin from 1986 to 2000 (14 years). A grid-based export coefficient model was used in the process of analysis based on to a geographic information system. The Songliao Basin is divided in four regions: Liaoning province, Jilin province (JL), Heilongjiang province and the eastern part of the Inner Mongolia (IM) Autonomous Region. Results indicated that the NPS phosphorus load caused by land use and non-land use increased steadily from 3.11×10(4) tons in 1986 to 3.49×10(4) tons in 2000. The southeastern region of the Songliao Plain was the most important NPS pollution contributor of all the districts. Although the TP load caused by land use decreased during the studied period in the Songliao River Basin, the contribution of land use to the TP load was dominant compared to non-land uses. The NPS pollution caused by non-land use steadily increased over the studied period. The IM Autonomous Region and JL province had the largest mean annual rate of change among all districts (more than 30%). In this area, livestock and poultry breeding had become one of the most important NPS pollution sources. These areas will need close attention in the future.

  16. The removal of nutrients from non-point source wastewater by a hybrid bioreactor.

    Science.gov (United States)

    Wu, Yonghong; Hu, Zhengyi; Yang, Linzhang; Graham, Bruce; Kerr, Philip G

    2011-02-01

    The aim of this project was to establish an economical and environmentally benign biotechnology for removing nutrients from non-point source wastewater. The proposal involves a hybrid bioreactor comprised of sequential anaerobic, anoxic and aerobic (A(2)/O) processes and an eco-ditch being constructed and applied in a suburban area, Kunming, south-western China, where wastewater was discharged from an industrial park and suburban communities. The results show that the hybrid bioreactor fosters heterotrophic and autotrophic microorganisms. When the hydraulic load is 200 m(3) per day with the running mode in 12h cycles, the removal efficiencies of the nutrients were 81% for TP, 74% for TDP, 82% for TN, 79% for NO(3)-N and 86% for NH(4)-N. The improved bacterial community structure and bacterial habitats further implied enhanced water quality and indicates that the easily-deployed, affordable and environmentally-friendly hybrid bioreactor is a promising bio-measure for removing high loadings of nutrients from non-point source wastewater.

  17. The Degree of Farmers’ Cognition on Non-point Source Pollution:Based on the Statistical Analysis of 453 Farmers

    Institute of Scientific and Technical Information of China (English)

    Qian; BI; Huaiye; WANG; Jue; PENG

    2014-01-01

    With the rapid development of modern agriculture,agricultural non-point source pollution becomes increasingly serious in China,improving farmers’ environmental protection consciousness plays a very important role in the reduction of agricultural non-point source pollution.Therefore,this investigation and study chooses rural areas of five counties from Chongqing city and Zhejiang province as our samples,our investigation and statistical analysis includes the following four aspects:farmers’ cognition on non-point source pollution concept,farmers’ cognition to rural environment satisfaction,farmers’ cognition on non-point source pollution in agricultural production and farmers’ cognition on the consequences of agricultural non-point source pollution and the effects on ecological environment.The analysis and conclusions of farmers’ consciousness cognition on agricultural non-point source pollution,provides the supports in theory and practice for optimizing the behavior of farmers,promoting the management of agricultural non-point source pollution and implementing new rural construction goal.

  18. A method to analyze "source-sink" structure of non-point source pollution based on remote sensing technology.

    Science.gov (United States)

    Jiang, Mengzhen; Chen, Haiying; Chen, Qinghui

    2013-11-01

    With the purpose of providing scientific basis for environmental planning about non-point source pollution prevention and control, and improving the pollution regulating efficiency, this paper established the Grid Landscape Contrast Index based on Location-weighted Landscape Contrast Index according to the "source-sink" theory. The spatial distribution of non-point source pollution caused by Jiulongjiang Estuary could be worked out by utilizing high resolution remote sensing images. The results showed that, the area of "source" of nitrogen and phosphorus in Jiulongjiang Estuary was 534.42 km(2) in 2008, and the "sink" was 172.06 km(2). The "source" of non-point source pollution was distributed mainly over Xiamen island, most of Haicang, east of Jiaomei and river bank of Gangwei and Shima; and the "sink" was distributed over southwest of Xiamen island and west of Shima. Generally speaking, the intensity of "source" gets weaker along with the distance from the seas boundary increase, while "sink" gets stronger.

  19. Modified Weighting for Calculating the Average Concentration of Non-Point Source Pollutant

    Institute of Scientific and Technical Information of China (English)

    牟瑞芳

    2004-01-01

    The concentration of runoff depends upon that of soil loss and the latter is assumed to be linear to the value of EI that equals the product of total storm energy E times the maximum 30-min intensity I30 for a given rainstorm. Usually, the maximum accumulative amount of rain for a rainstorm might bring on the maximum amount of runoff, but it does not equal the maximum erosion and not always lead the maximum concentration. Thus, the average concentration weighted by amount of runoff is somewhat unreasonable. An improvement for the calculation method of non-point source pollution load put forward by professor Li Huaien is proposed. In replacement of the weight of runoff, EI value of a single rainstorm is introduced as a new weight. An example of Fujing River watershed shows that its application is effective.

  20. Isotopic Tracers for Delineating Non-Point Source Pollutants in Surface Water

    Energy Technology Data Exchange (ETDEWEB)

    Davisson, M L

    2001-03-01

    This study tested whether isotope measurements of surface water and dissolved constituents in surface water could be used as tracers of non-point source pollution. Oxygen-18 was used as a water tracer, while carbon-14, carbon-13, and deuterium were tested as tracers of DOC. Carbon-14 and carbon-13 were also used as tracers of dissolved inorganic carbon, and chlorine-36 and uranium isotopes were tested as tracers of other dissolved salts. In addition, large databases of water quality measurements were assembled for the Missouri River at St. Louis and the Sacramento-San Joaquin Delta in California to enhance interpretive results of the isotope measurements. Much of the water quality data has been under-interpreted and provides a valuable resource to investigative research, for which this report exploits and integrates with the isotope measurements.

  1. Investigation and Analysis of Guangzhou Nansha Coast Park Point Source Pollution and Non-point Source Pollution

    Institute of Scientific and Technical Information of China (English)

    Ruijie; YANG; Huanghuang; ZENG; Budan; CHEN; Fang; CHEN; Shikai; WU

    2014-01-01

    [Objective]To find out the situation of Nansha Coast Park point and non-point source pollution.[Method]By investigating the park water environment,analysis of point and non-point source pollutants contribution rate,setting up water quality monitoring sites for basic data related indicators and then using national water quality standards to evaluate water quality.[Result]The Coast Park point source pollution mainly comes from the campus greeting fertilizer spraying.The COD of lakes and river outside the park and ammonia mean concentration belong to grade III.The total nitrogen of lake belongs to grade III.The total phosphorus belongs to grade IV.The total nitrogen of river is the worst.The total phosphorus is grade V.[Conclusion] The lake water quality is highly affected by the point and non-point source pollution,the quality of the river is worse than that of the lake in the park,and it needs powerful governance.

  2. Evaluating the Effects of Land Use Planning for Non-Point Source Pollution Based on a System Dynamics Approach in China.

    Science.gov (United States)

    Kuai, Peng; Li, Wei; Liu, Nianfeng

    2015-01-01

    Urbanization is proceeding rapidly in several developing countries such as China. This accelerating urbanization alters the existing land use types in a way that results in more Non-Point Source (NPS) pollution to local surface waters. Reasonable land use planning is necessary. This paper compares seven planning scenarios of a case study area, namely Wulijie, China, from the perspective of NPS pollution. A System Dynamics (SD) model was built for the comparison to adequately capture the planning complexity. These planning scenarios, which were developed by combining different land use intensities (LUIs) and construction speeds (CSs), were then simulated. The results show that compared to scenario S1 (business as usual) all other scenarios will introduce more NPS pollution (with an incremental rate of 22%-70%) to Wulijie. Scenario S6 was selected as the best because it induced relatively less NPS pollution while simultaneously maintaining a considerable development rate. Although LUIs represent a more critical factor compared to CSs, we conclude that both LUIs and CSs need to be taken into account to make the planning more environmentally friendly. Considering the power of SD in decision support, it is recommended that land use planning should take into consideration findings acquired from SD simulations.

  3. Optimization strategy integrity for watershed agricultural non-point source pollution control based on Monte Carlo simulation

    Science.gov (United States)

    Gong, Y.; Yu, Y. J.; Zhang, W. Y.

    2016-08-01

    This study has established a set of methodological systems by simulating loads and analyzing optimization strategy integrity for the optimization of watershed non-point source pollution control. First, the source of watershed agricultural non-point source pollution is divided into four aspects, including agricultural land, natural land, livestock breeding, and rural residential land. Secondly, different pollution control measures at the source, midway and ending stages are chosen. Thirdly, the optimization effect of pollution load control in three stages are simulated, based on the Monte Carlo simulation. The method described above is applied to the Ashi River watershed in Heilongjiang Province of China. Case study results indicate that the combined three types of control measures can be implemented only if the government promotes the optimized plan and gradually improves implementation efficiency. This method for the optimization strategy integrity for watershed non-point source pollution control has significant reference value.

  4. Spatio-temporal variation of erosion-type non-point source pollution in a small watershed of hilly and gully region, Chinese Loess Plateau.

    Science.gov (United States)

    Wu, Lei; Liu, Xia; Ma, Xiao-Yi

    2016-06-01

    Loss of nitrogen and phosphorus in the hilly and gully region of Chinese Loess Plateau not only decreases the utilization rate of fertilizer but also is a potential threat to aquatic environments. In order to explore the process of erosion-type non-point source (NPS) pollution in Majiagou watershed of Loess Plateau, a distributed, dynamic, and integrated NPS pollution model was established to investigate impacts of returning farmland on erosion-type NPS pollution load from 1995 to 2012. Results indicate that (1) the integrated model proposed in this study was verified to be reasonable; the general methodology is universal and can be applicable to the hilly and gully region, Loess Plateau; (2) the erosion-type NPS total nitrogen (TN) and total phosphorus (TP) load showed an overall decreasing trend; the average nitrogen and phosphorus load modulus in the last four years (2009-2012) were 1.23 and 1.63 t/km(2) · a, respectively, which were both decreased by about 35.4 % compared with the initial treatment period (1995-1998); and (3) The spatial variations of NPS pollution are closely related to spatial characteristics of rainfall, topography, and soil and land use types; the peak regions of TN and TP loss mainly occurred along the main river banks of the Yanhe River watershed from northeast to southeast, and gradually decreased with the increase of distance to the left and right river banks, respectively. Results may provide scientific basis for the watershed-scale NPS pollution control of the Loess Plateau.

  5. Pollution of surface waters by metalaxyl and nitrate from non-point sources.

    Science.gov (United States)

    Bermúdez-Couso, Alipio; Fernández-Calviño, David; Álvarez-Enjo, Manuel Ali; Simal-Gándara, Jesús; Nóvoa-Muñoz, Juan Carlos; Arias-Estévez, Manuel

    2013-09-01

    The mobility of contaminants in soil is highly dependent upon the characteristics of the contaminant chemical and the properties of the soil. In order to explore these relationships, the district of A Limia (Galicia, NW Spain) was selected as the study area--a cropland devoted to growing potatoes, where the soil had been managed intensively over the last 50 years. The soil was characterised by low slopes with the water table located very close to the soil surface. Our aim was to study the influence of high and intensive crop production on the water bodies and non-point source contamination, with a particular focus on metalaxyl and nitrate. The highest concentrations of metalaxyl occurred when rainfalls were low and in zones of the study area where natural hydrology was significantly altered by numerous drainage canals. The spatial and temporal distributions of the nitrate also showed a high variability, with the interaction between seasons and sampling area being the most significant factor in explaining the levels found. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. A distributed non-point source pollution model: calibration and validation in the Yellow River Basin.

    Science.gov (United States)

    Hao, Fang-hua; Zhang, Xue-song; Yang, Zhi-feng

    2004-01-01

    The applicability of a non-point source pollution model--SWAT(soil and water assessment tools) in a large river basin with high sediment runoff modulus(770 t/km2 in the Yellow River) was examined. The basic database, which includes DEM, soil and landuse map, weather data, and land management data, was established for the study area using GIS. A two-stage "Brute Force" optimization method was used to calibrate the parameters with the observed monthly flow and sediment data from 1992 to 1997. In the process of calibration automated digital filter technique was used to separate direct runoff and base flow. The direct runoff was firstly calibrated, and the base flow, then the total runoff was matched. The sediment yield was calibrated to match well. Keeping input parameters set during the calibration process unchanged, the model was validated with 1998--1999's observed monthly flow and sediment. The evaluation coefficients for simulated and observed flow and sediment showed that SWAT was successfully applied in the study area: relative error was within 20%, coefficient of determination and Nash-Suttcliffe simulation efficiency were all equal to or above 0.70 during calibration and validation period.

  7. Model AVSWAT apropos of simulating non-point source pollution in Taihu lake basin.

    Science.gov (United States)

    Zhang, Qiu-Ling; Chen, Ying-Xu; Jilani, Ghulam; Shamsi, Imran Haider; Yu, Qiao-Gang

    2010-02-15

    Accelerated eutrophication and nutrient loads in the lakes are of major concern for human health and environment. This study was undertaken for modeling the non-point source pollution of Taihu lake basin in eastern China. The SWAT model having an interface in ArcView GIS was employed. Model sensitive parameters related to hydrology and water quality were obtained by sensitivity analysis, and then calibrated and validated by comparing model predictions with field data. The GIS showed good potential for parameterization of hill-slopes, channels, and representative slope profiles for SWAT model simulations. In a monthly and daily time step, the model's Nash-Sutcliffe coefficient (E) and the coefficient of determination (R(2)) indicated that values of simulated runoff, NH(4)(+)-N and total phosphorus were acceptably closer to the measured data. Surface water parameters especially CN, Soil-AWC and ESCO were the most sensitive and had more recognition in the model. It is concluded that runoff carrying N and P nutrients from chemical fertilizer inputs in agricultural areas is the major contributor to NPSP in the lake basin. So, decrease in excessive use of N and P fertilizers and their synergism with organic manures is recommended that would significantly reduce nutrient pollution in the lake ecosystem.

  8. Reducing future non-point source sediment and phosphorus loading under intensifying agricultural production in the Ethiopian highlands

    Science.gov (United States)

    Mogus, Mamaru; Schmitter, Petra; Tilahun, Seifu; Steenhuise, Tammo

    2016-04-01

    Intensification of agriculture will bring along non-point source pollution in the Ethiopian highlands resulting in eutrophication of lakes. The first signs of eutrophication have been observed already in Lake Tana. The lake it supports the lives of millions in the surrounding through fishing, tourism, transportation and hydropower.Presently, information on non-point source pollution is lacking in the Ethiopian highlands. There are few studies carried out in the highlands on the extent and the source areas of pollution, and models are not available for predicting sediment and phosphorus loading other than those developed for temperate climates. The objective of this chapter is to review existing non-point source studies, report on our findings of sediment and phosphorus sources that are related the non-point source pollution of Lake Tana and to present a non-point source model for the Ethiopian highland based on the Parameter Efficient Semi-distributed Watershed Hydrology Model (PED-WHM).In our research we have found that the saturation excess runoff from valley bottoms and from degraded lands are prevalent in the Ethiopia highlands. The periodically runoff source areas are also the sources for the non-point source pollution and by concentrating best management practices in these source areas we expect that we can reduce pollution without affecting the profitability of the existing farms. The water balance component of the non-point source model has been performing well in predicting both the discharge and the location of the runoff source areas. Sediment and phosphorus prediction models have been developed and are currently being tested for the 7km2Awramba watershed and the 1350 km2Gumara basin. Initial results indicate that 11.2 ton/ha/year sediment load and an accumulation rate of 17.3 mg/kg/year of dissolved phosphorus from Gumara watershed joining the lake. By developing best management practices at this time before non-point source pollution is rampant and

  9. Long-term variation (1960-2003) and causal factors of non-point-source nitrogen and phosphorus in the upper reach of the Yangtze River.

    Science.gov (United States)

    Shen, Zhenyao; Chen, Lei; Ding, Xiaowen; Hong, Qian; Liu, Ruimin

    2013-05-15

    The knowledge of long-term variation and causal factors of non-point source (NPS) pollution in large-scale watersheds is helpful in the development of water quality control programs. In this study, the Improved Export Coefficient Model and the Revised Universal Soil Loss Equation were combined to estimate the temporal and spatial variations (1960-2003) of NPS pollution in the upper reach of the Yangtze River (URYR). Two change points for NPS pollution were successfully detected. In the URYR, the dissolved nitrogen (DN) and dissolved phosphorus (DP) increased before 2000 and decreased after 2000, whereas the inflection points from increase to decline were around 1980 for the adsorbed N (AN) and adsorbed P (AP). The results also indicated that the dissolved pollutants were mainly contributed by the anthropogenic factors, while the adsorbed pollutants were primarily exported by the natural factors. By comparing the load intensities from each source, it revealed that for the dissolved pollutants, the major source of the high load intensity transferred from urban land to dry land after 1980. Simultaneously, the high load intensity areas of the adsorbed pollutants transferred from forest to orchard around 1980, which was mainly attributed to the increasing fertilizer application. These results may be useful for planning and management of the URYR and other large-scale watersheds.

  10. Application of modified export coefficient method on the load estimation of non-point source nitrogen and phosphorus pollution of soil and water loss in semiarid regions.

    Science.gov (United States)

    Wu, Lei; Gao, Jian-en; Ma, Xiao-yi; Li, Dan

    2015-07-01

    Chinese Loess Plateau is considered as one of the most serious soil loss regions in the world, its annual sediment output accounts for 90 % of the total sediment loads of the Yellow River, and most of the Loess Plateau has a very typical characteristic of "soil and water flow together", and water flow in this area performs with a high sand content. Serious soil loss results in nitrogen and phosphorus loss of soil. Special processes of water and soil in the Loess Plateau lead to the loss mechanisms of water, sediment, nitrogen, and phosphorus are different from each other, which are greatly different from other areas of China. In this study, the modified export coefficient method considering the rainfall erosivity factor was proposed to simulate and evaluate non-point source (NPS) nitrogen and phosphorus loss load caused by soil and water loss in the Yanhe River basin of the hilly and gully area, Loess Plateau. The results indicate that (1) compared with the traditional export coefficient method, annual differences of NPS total nitrogen (TN) and total phosphorus (TP) load after considering the rainfall erosivity factor are obvious; it is more in line with the general law of NPS pollution formation in a watershed, and it can reflect the annual variability of NPS pollution more accurately. (2) Under the traditional and modified conditions, annual changes of NPS TN and TP load in four counties (districts) took on the similar trends from 1999 to 2008; the load emission intensity not only is closely related to rainfall intensity but also to the regional distribution of land use and other pollution sources. (3) The output structure, source composition, and contribution rate of NPS pollution load under the modified method are basically the same with the traditional method. The average output structure of TN from land use and rural life is about 66.5 and 17.1 %, the TP is about 53.8 and 32.7 %; the maximum source composition of TN (59 %) is farmland; the maximum source

  11. Agricultural non-point source pollution of glyphosate and AMPA at a catchment scale

    Science.gov (United States)

    Okada, Elena; Perez, Debora; De Geronimo, Eduardo; Aparicio, Virginia; Costa, Jose Luis

    2017-04-01

    Information on the actual input of pesticides into the environment is crucial for proper risk assessment and the design of risk reduction measures. The Crespo basin is found within the Balcarce County, located south-east of the Buenos Aires Province. The whole basin has an area of approximately 490 km2 and the river has a length of 65 km. This study focuses on the upper basin of the Crespo stream, covering an area of 226 km2 in which 94.7% of the land is under agricultural production representing a highly productive area, characteristic of the Austral Pampas region. In this study we evaluated the levels of glyphosate and its metabolite aminomethylphosphonic acid (AMPA) in soils; and the non-point source pollution of surface waters, stream sediments and groundwater, over a period of one year. Stream water samples were taken monthly using propylene bottles, from the center of the bridge. If present, sediment samples from the first 5 cm were collected using cylinder samplers. Groundwater samples were taken from windmills or electric pumps from different farms every two months. At the same time, composite soil samples (at 5 cm depth) were taken from an agricultural plot of each farm. Samples were analyzed for detection and quantification of glyphosate and AMPA using ultra-performance liquid chromatography coupled to a mass spectrometer (UPLC-MS/MS). The limit of detection (LD) in the soil samples was 0.5 μg Kg-1 and the limit of quantification (LQ) was 3 μg Kg-1, both for glyphosate and AMPA. In water samples the LD was 0.1 μg L-1 and the LQ was 0.5 μg L-1. The results showed that the herbicide dispersed into all the studied environmental compartments. Glyphosate and AMPA residues were detected in 34 and 54% of the stream water samples, respectively. Sediment samples had a higher detection frequency (>96%) than water samples, and there was no relationship between the presence in surface water with the detection in sediment samples. The presence in sediment samples

  12. Assessing the effects of non-point source pollution on American Samoa's coral reef communities.

    Science.gov (United States)

    Houk, Peter; Didonato, Guy; Iguel, John; Van Woesik, Robert

    2005-08-01

    Surveys were completed on Tutuila Island, American Samoa, to characterize reef development and assess the impacts of non-point source pollution on adjacent coral reefs at six sites. Multivariate analyses of benthic and coral community data found similar modern reef development at three locations; Aoa, Alofau, and Leone. These sites are situated in isolated bays with gentle sloping foundations. Aoa reefs had the highest estimates of crustose coralline algae cover and coral species richness, while Leone and Alofau showed high abundances of macroalgae and Porites corals. Aoa has the largest reef flat between watershed discharge and the reef slope, and the lowest human population density. Masefau and Fagaalu have a different geomorphology consisting of cemented staghorn coral fragments and steep slopes, however, benthic and coral communities were not similar. Benthic data suggest Fagaalu is heavily impacted compared with all other sites. Reef communities were assessed as bio-criteria indicators for waterbody health, using the EPA aquatic life use support designations of (1) fully supportive, (2) partially supportive, and (3) non-supportive for aquatic life. All sites resulted in a partially supportive ranking except Fagaalu, which was non-supportive. The results of this rapid assessment based upon relative benthic community measures are less desirable than long-term dataset analyses from monitoring programs, however it fills an important role for regulatory agencies required to report annual waterbody assessments. Future monitoring sites should be established to increase the number of replicates within each geological and physical setting to allow for meaningful comparisons along a gradient of hypothesized pollution levels.

  13. Spatial and Temporal Distribution Characteristics of Agricultural Non-point Source Pollution in Xixi Watershed of Jinjiang Basin

    Institute of Scientific and Technical Information of China (English)

    Kun RONG; Jiqiang ZHANG; Yang SHI

    2016-01-01

    The SWAT model was applied to analyze the temporal-spatial distribution patterns of non-point source pollution loads and the difference of pollution loads of different land use types in Xixi Watershed of Jinjiang Basin. The results showed that both yearly nitrogen and phosphorus pollution loads were evenly distributed during 1973 to 1979,the annual TN pollution from non-point source was 1530 t,or 6. 3 kg / ha,and the annual TP pollution from non-point source was 270 t,or 1. 1 kg / ha during 1973 to 1979 in the watershed. Considerable differences were identified on both monthly nitrogen and phosphorus pollution loads. The TN and TP pollution loads during the flood season( from April to September) accounted for 76. 2% and 75. 8% of the annual load respectively. There were great differences in both TN and TP pollution loads of different land use types in the study area,and the pollution load of both farmland and orchard was higher than that of the other land use types. TN and TP pollution loads of farmland accounted for 66% and 83% of total watershed. There was a great spatial difference in the nonpoint source pollution load of the study area. The critical source areas of non-point source pollution are mainly located at Guanqiao Town,Longmen Town,Changkeng Town,Shangqing Town and Dapu Town,where the efforts of controlling pollution should be made.

  14. Contingent Valuation of Residents' Attitudes and Willingness-to-Pay for Non-point Source Pollution Control: A Case Study in AL-Prespa, Southeastern Albania

    Science.gov (United States)

    Grazhdani, Dorina

    2015-07-01

    Recently, local governments in Albania have begun paying attention to management of small watershed, because there are specific boundaries and people living within a watershed basin tend to be more concerned about the basin's environmental, economic, and social development. But this natural resource management and non-point source (NPS) pollution control is still facing challenges. Albanian part of Prespa Park (AL-Prespa) is a good case study, as it is a protected wetland area of high biodiversity and long human history. In this framework, this study was undertaken, the main objectives of which were to explore: (1) the attitudes of the residents toward NPS pollution control, (2) their willingness-to-pay for improving water quality, and (3) factors affecting the residents' willingness-to-pay. Descriptive statistics, one-way ANOVA (analysis of variance), Chi-square analysis, and multivariate data analysis techniques were used. Findings strongly suggested that the residents' attitudes toward NPS pollution control in this area were positive. With the combination of two major contingent valuation methods—dichotomous choice and open-ended formats, the survey results indicated that the average yearly respondents' WTP was €6.4. The survey revealed that residents' yearly income and education level were the main factors affecting residents' willingness-to-pay for NPS pollution control in this area, and there was no significant correlation between residents' yearly income and their education level. The current study would lay a solid foundation on decision-making in further NPS pollution control and public participation through community-based watershed management policies in AL-Prespa watershed and similar areas.

  15. The simulation research of dissolved nitrogen and phosphorus non-point source pollution in Xiao-Jiang watershed of Three Gorges Reservoir area.

    Science.gov (United States)

    Wu, Lei; Long, Tian-Yu; Li, Chong-Ming

    2010-01-01

    Xiao-jiang, with a basin area of almost 5,276 km(2) and a length of 182.4 km, is located in the center of the Three Gorges Reservoir Area, and is the largest tributary of the central section in Three Gorges Reservoir Area, farmland accounts for a large proportion of Xiao-jiang watershed, and the hilly cropland of purple soil is much of the farmland of the watershed. After the second phase of water storage in the Three Gorges Reservoir, the majority of sub-rivers in the reservoir area experienced eutrophication phenomenon frequently, and non-point source (NPS) pollution has become an important source of pollution in Xiao-jiang Watershed. Because dissolved nitrogen and phosphorus non-point source pollution are related to surface runoff and interflow, using climatic, topographic and land cover data from the internet and research institutes, the Semi-Distributed Land-use Runoff Process (SLURP) hydrological model was introduced to simulate the complete hydrological cycle of the Xiao-jiang Watershed. Based on the SLURP distributed hydrological model, non-point source pollution annual output load models of land use and rural residents were respectively established. Therefore, using GIS technology, considering the losses of dissolved nitrogen and phosphorus in the course of transport, a dissolved non-point source pollution load dynamic model was established by the organic coupling of the SLURP hydrological model and land-use output model. Through the above dynamic model, the annual dissolved non-point source nitrogen and phosphorus pollution output as well as the load in different types were simulated and quantitatively estimated from 2001 to 2008, furthermore, the loads of Xiao-jiang Watershed were calculated and expressed by temporal and spatial distribution in the Three Gorges Reservoir Area. The simulation results show that: the temporal changes of dissolved nitrogen and phosphorus load in the watershed are close to the inter-annual changes of rainfall runoff, and the

  16. The Treatment Train approach to reducing non-point source pollution from agriculture

    Science.gov (United States)

    Barber, N.; Reaney, S. M.; Barker, P. A.; Benskin, C.; Burke, S.; Cleasby, W.; Haygarth, P.; Jonczyk, J. C.; Owen, G. J.; Snell, M. A.; Surridge, B.; Quinn, P. F.

    2016-12-01

    An experimental approach has been applied to an agricultural catchment in NW England, where non-point pollution adversely affects freshwater ecology. The aim of the work (as part of the River Eden Demonstration Test Catchment project) is to develop techniques to manage agricultural runoff whilst maintaining food production. The approach used is the Treatment Train (TT), which applies multiple connected mitigation options that control nutrient and fine sediment pollution at source, and address polluted runoff pathways at increasing spatial scale. The principal agricultural practices in the study sub-catchment (1.5 km2) are dairy and stock production. Farm yards can act as significant pollution sources by housing large numbers of animals; these areas are addressed initially with infrastructure improvements e.g. clean/dirty water separation and upgraded waste storage. In-stream high resolution monitoring of hydrology and water quality parameters showed high-discharge events to account for the majority of pollutant exports ( 80% total phosphorus; 95% fine sediment), and primary transfer routes to be surface and shallow sub-surface flow pathways, including drains. To manage these pathways and reduce hydrological connectivity, a series of mitigation features were constructed to intercept and temporarily store runoff. Farm tracks, field drains, first order ditches and overland flow pathways were all targeted. The efficacy of the mitigation features has been monitored at event and annual scale, using inflow-outflow sampling and sediment/nutrient accumulation measurements, respectively. Data presented here show varied but positive results in terms of reducing acute and chronic sediment and nutrient losses. An aerial fly-through of the catchment is used to demonstrate how the TT has been applied to a fully-functioning agricultural landscape. The elevated perspective provides a better understanding of the spatial arrangement of mitigation features, and how they can be

  17. Comparative study on nutrient removal of agricultural non-point source pollution for three filter media filling schemes in eco-soil reactors.

    Science.gov (United States)

    Du, Fuyi; Xie, Qingjie; Fang, Longxiang; Su, Hang

    2016-08-01

    Nutrients (nitrogen and phosphorus) from agricultural non-point source (NPS) pollution have been increasingly recognized as a major contributor to the deterioration of water quality in recent years. The purpose of this article is to investigate the discrepancies in interception of nutrients in agricultural NPS pollution for eco-soil reactors using different filling schemes. Parallel eco-soil reactors of laboratory scale were created and filled with filter media, such as grit, zeolite, limestone, and gravel. Three filling schemes were adopted: increasing-sized filling (I-filling), decreasing-sized filling (D-filling), and blend-sized filling (B-filling). The systems were intermittent operations via simulated rainstorm runoff. The nutrient removal efficiency, biomass accumulation and vertical dissolved oxygen (DO) distribution were defined to assess the performance of eco-soil. The results showed that B-filling reactor presented an ideal DO for partial nitrification-denitrification across the eco-soil, and B-filling was the most stable in the change of bio-film accumulation trends with depth in the three fillings. Simultaneous and highest removals of NH4(+)-N (57.74-70.52%), total nitrogen (43.69-54.50%), and total phosphorus (42.50-55.00%) were obtained in the B-filling, demonstrating the efficiency of the blend filling schemes of eco-soil for oxygen transfer and biomass accumulation to cope with agricultural NPS pollution.

  18. Non-point Source Pollution Modeling Using Geographic Information System (GIS for Representing Best Management Practices (BMP in the Gorganrood Watershed

    Directory of Open Access Journals (Sweden)

    Z. Pasandidehfard

    2014-09-01

    Full Text Available The most important pollutants that cause water pollution are nitrogen and phosphorus from agricultural runoff called Non-Point Source Pollution (NPS. To solve this problem, management practices known as BMPs or Best Management Practices are applied. One of the common methods for Non-Point Source Pollution prediction is modeling. By modeling, efficiency of many practices can be tested before application. In this study, land use changes were studied from the years 1984 till 2010 that showed an increase in agricultural lands from 516908.52 to 630737.19 ha and expansion of cities from 5237.87 to 15487.59 ha and roads from 9666.07 to 11430.24 ha. Using L-THIA model (from nonpoint source pollution models for both land use categories, the amount of pollutant and the volume of runoff were calculated that showed high growth. Then, the seventh sub-basin was recognized as a critical zone in terms of pollution among the sub-basins. In the end, land use change was considered as a BMP using Multi-Criteria Evaluation (MCE based on which a more suitable land use map was produced. After producing the new land use map, L-THIA model was run again and the result of the model was compared to the actual land use to show the effect of this BMP. Runoff volume decreased from 367.5 to 308.6 M3/ha and nitrogen in runoff was reduced from 3.26 to 1.58 mg/L and water BOD from 3.61 to 2.13 mg/L. Other pollutants also showed high reduction. In the end, land use change is confirmed as an effective BMP for Non-Point Source Pollution reduction.

  19. [Spatial discharge characteristics and total load control of non-point source pollutants based on the catchment scale].

    Science.gov (United States)

    Wang, Xia-Hui; Lu, Jun; Zhang, Qing-Zhong; Wang, Bo; Yao, Rui-Hua; Zhang, Hui-Yuan; Huang, Feng

    2011-09-01

    Agricultural non-point source pollution is one of the major causes of water quality deterioration. Based on the analysis of the spatial discharge characteristics and intensity of major pollutants from the agricultural pollution source, the establishment of spatial management subzones for controlling agricultural non-point pollution and a design of a plan for total load control of pollutants from each subzone is an important way to improve the efficiency of control measures. In this paper the Four Lake basin in Hubei Province is adopted as the research case region and a systematic research of the control countermeasures of agricultural non-point pollution based on the catchment scale is carried out. The results shows that in the Four Lake basin, the COD, total nitrogen, total phosphorus and ammonia nitrogen load of the water environment are mainly caused by agricultural non-point pollution. These four kinds of non-point source pollutants respectively account for 67.6%, 82.2%, 84.7% and 50.9% of the total pollutant discharge amount in the basin. The analysis of the spatial discharge characteristics of non-point source pollutants in the Four Lake basin shows that the major contributor source regions of non-point source pollutant in the basin are the four counties, including Honghu, Jianli, Qianjiang and Shayang where the aquatic and livestock production are relatively developed. According to the spatial discharge characteristics of the pollutants and the evaluation of the discharge intensity of pollutants, the Four Lake basin is divided into three agricultural non-point pollution management subzones, which including Changhu upstream aquatic and livestock production pollution control subzone, Four-lake trunk canal rural non-point source pollution control subzone and Honghu aquatic production pollution control subzone. Specific pollution control measures are put forward for each subzone. With a comprehensive consideration of the water quality amelioration and the

  20. [Three patterns of interaction between soil and non-point source P-pollutants in agricultural watershed].

    Science.gov (United States)

    Wang, Xia-hui; Yin, Cheng-qing; Yan, Xiao; Shan, Bao-qing; Wang, Wei-dong

    2004-07-01

    Typical agricultural watershed was selected to study the interactions between soil matrix and non-point source P-pollutants in surface runoff under simulative conditions. The soil samples were taken in different spatial locations in this watershed and were under different degree of human disturbance. The results showed that the interactions between different soil matrix and phosphorus could be divided into three patterns:retention, release and combination of retention and release. Soil of retention pattern has strong adsorption capacity of phosphate and will retain phosphorus from polluted runoff. Soil of release pattern has significant desorption capacity of phosphate and will release phosphorus to the runoff. Soil of retention and release combination pattern will retain or release phosphorus according to the phosphate concentration in the polluted runoff. These results showed that soil matrix in different spatial locations in the agricultural watershed have different ecological functions and environmental values under the processing of natural conditions and human disturbance. From the view of occurrence of non-point source pollution, these soils could become the sink of pollutants as well as the source of pollutants. Under some conditions, there has a conversion between sink and source of them. These results are valuable for control of non-point source pollution on watershed level, identification of key source area of pollutants and improvement of efficiency of control measures.

  1. SPATIAL ANALYSIS OF SOIL EROSION AND NON-POINT SOURCE POLLUTION BASED ON GIS IN ERLONG LAKE WATERSHED, JILIN PROVINCE

    Institute of Scientific and Technical Information of China (English)

    WANG Ning; ZHANG Hong-yan; WANG Hui-lian; ZHANG Zheng-xiang

    2004-01-01

    Data collection, factor composition, nappe analysis and integrative simulation of natural geographical factors in Erlong Lake watershed have been carried out based on GIS. The risk areas where non-point source pollution may occur were compartmentalized and assessed, and the total soil erosion and the runoffs of N and P with rainfall in this valley were worked out by experiment and GIS mapping. The study indicated that the main type of soil in dry land with variable slope east of the lake and the middle-south parts of steep slope mountainous region (erosion source pollution (NSP) of nitrogen and phosphorus loss was corresponded with the soil erosion. Spatial distribution and the reasons of the distribution difference have been presented and it was emphasized that the human activities among the influence factors was the most important. It surely offers a scientific basis to control and prevent non-point source pollution in the watershed.

  2. Role of rural solid waste management in non-point source pollution control of Dianchi Lake catchments, China

    Institute of Scientific and Technical Information of China (English)

    Wenjing LU; Hongtao WANG

    2008-01-01

    In recent years, with control of the main municipal and industrial point pollution sources and implementation of cleaning for some inner pollution sources in the water body, the discharge of point source pollution decreased gradually, while non-point source pollution has become increasingly distressing in Dianchi Lake catchments. As one of the major targets in non-point source pollution control, an integrated solid waste controlling strategy combined with a technological solution and management system was proposed and implemented based on the waste disposal situation and characteristics of rural solid waste in the demonstration area. As the key technoogy in rural solid waste treatment, both centralized plantscale composting and a dispersed farmer-operated waste treating system showed promise in rendering timely benefits in efficiency, large handling capacity, high quality of the end product, as well as good economic return. Problems encountered during multi-substrates co-com-posting such as pathogens, high moisture content, asyn-chronism in the decomposition of different substrates, and low quality of the end product can all be tackled. 92.5% of solid waste was collected in the demonstration area, while the treating and recycling ratio reached 87.9%, which pre-vented 32.2 t nitrogen and 3.9 t phosphorus per year from entering the water body of Dianchi Lake after imple-mentation of the project.

  3. Anthropogenic point-source and non-point-source nitrogen inputs into Huai River basin and their impacts on riverine ammonia-nitrogen flux

    Science.gov (United States)

    Zhang, W. S.; Swaney, D. P.; Li, X. Y.; Hong, B.; Howarth, R. W.; Ding, S. H.

    2015-07-01

    This study provides a new approach to estimate both anthropogenic non-point-source and point-source nitrogen (N) inputs to the landscape, and determines their impacts on riverine ammonia-nitrogen (AN) flux, providing a foundation for further exploration of anthropogenic effects on N pollution. Our study site is Huai River basin of China, a water-shed with one of the highest levels of N input in the world. Multi-year average (2003-2010) inputs of N to the watershed are 27 200 ± 1100 kg N km-2 yr-1. Non-point sources comprised about 98 % of total N input, and only 2 % of inputs are directly added to the aquatic ecosystem as point sources. Fertilizer application was the largest non-point source of new N to the Huai River basin (69 % of net anthropogenic N inputs), followed by atmospheric deposition (20 %), N fixation in croplands (7 %), and N content of imported food and feed (2 %). High N inputs showed impacts on riverine AN flux: fertilizer application, point-source N input, and atmospheric N deposition were proved as more direct sources to riverine AN flux. Modes of N delivery and losses associated with biological denitrification in rivers, water consumption, interception by dams may influence the extent of export of riverine AN flux from N sources. Our findings highlight the importance of anthropogenic N inputs from both point sources and non-point sources in heavily polluted watersheds, and provide some implications for AN prediction and management.

  4. Modelling of point and non-point source pollution of nitrate with SWAT in the river Dill, Germany

    OpenAIRE

    T. Pohlert; J. A. Huisman; L. Breuer; Frede, H.-G.

    2005-01-01

    International audience; We used the Soil and Water Assessment Tool (SWAT) to simulate point and non-point source pollution of nitrate in a mesoscale mountainous catchment. The results show that the model efficiency for daily discharge is 0.81 for the calibration period (November 1990 to December 1993) and 0.56 for the validation period (April 2000 to January 2003). The model efficiency for monthly nitrate load is 0.66 and 0.77 for the calibration period (April 2000 to March 2002) and validati...

  5. Study on the Control Model of Rural Non-point Source Pollution——Taking Ninghe County in Tianjin as an Example

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective] The aim was to study the control model of rural non-point source pollution.[Method] Taking Ninghe County(a typical agricultural county in Tianjin) as an example,the current development of local economy and society and characteristics of rural non-point source pollution were studied firstly,then the control model of rural non-point source pollution suitable for Ninghe County was constructed,and its environmental and economic benefits were analyzed finally.[Result] According to the sources of non-...

  6. User's Guide for the Agricultural Non-Point Source (AGNPS) Pollution Model Data Generator

    Science.gov (United States)

    Finn, Michael P.; Scheidt, Douglas J.; Jaromack, Gregory M.

    2003-01-01

    BACKGROUND Throughout this user guide, we refer to datasets that we used in conjunction with developing of this software for supporting cartographic research and producing the datasets to conduct research. However, this software can be used with these datasets or with more 'generic' versions of data of the appropriate type. For example, throughout the guide, we refer to national land cover data (NLCD) and digital elevation model (DEM) data from the U.S. Geological Survey (USGS) at a 30-m resolution, but any digital terrain model or land cover data at any appropriate resolution will produce results. Another key point to keep in mind is to use a consistent data resolution for all the datasets per model run. The U.S. Department of Agriculture (USDA) developed the Agricultural Nonpoint Source (AGNPS) pollution model of watershed hydrology in response to the complex problem of managing nonpoint sources of pollution. AGNPS simulates the behavior of runoff, sediment, and nutrient transport from watersheds that have agriculture as their prime use. The model operates on a cell basis and is a distributed parameter, event-based model. The model requires 22 input parameters. Output parameters are grouped primarily by hydrology, sediment, and chemical output (Young and others, 1995.) Elevation, land cover, and soil are the base data from which to extract the 22 input parameters required by the AGNPS. For automatic parameter extraction, follow the general process described in this guide of extraction from the geospatial data through the AGNPS Data Generator to generate input parameters required by the pollution model (Finn and others, 2002.)

  7. Modelling of point and non-point source pollution of nitrate with SWAT in the river Dill, Germany

    Directory of Open Access Journals (Sweden)

    T. Pohlert

    2005-01-01

    Full Text Available We used the Soil and Water Assessment Tool (SWAT to simulate point and non-point source pollution of nitrate in a mesoscale mountainous catchment. The results show that the model efficiency for daily discharge is 0.81 for the calibration period (November 1990 to December 1993 and 0.56 for the validation period (April 2000 to January 2003. The model efficiency for monthly nitrate load is 0.66 and 0.77 for the calibration period (April 2000 to March 2002 and validation period (April 2002 to January 2003, respectively. However, the model efficiency for daily loads is low (0.15, which cannot only be attributed to the quality of input data of point source effluents. An analysis of the internal fluxes and cycles of nitrogen pointed out considerable weaknesses in the models conceptualisation of the nitrogen modules which will be improved in future research.

  8. Search for the northwest passage: the assignation of NSP (non-point source pollution) rights in nutrient trading programs.

    Science.gov (United States)

    Collentine, D

    2002-01-01

    The search for solutions to the problem of non-point source pollution (NSP) includes alternatives based on theories associated with the use of tradable pollution permits. Tradable permit programs have received significant support as a promising policy for the reduction of effluent discharges but programs in practice have not been regarded as successful. The lack of success is ascribed to the design of the programs. However, this may be a design problem which is insurmountable due to the nature of the NSP problem. Tradable permit solutions are based on an assumption that the assignation of quantifiable rights to both point and nonpoint sources, based on some predetermined ambient water quality measure, is possible. The conclusion here is that there are significant features particular to NSP that hinder the introduction of rights and significantly decrease the utility of tradable permit solutions.

  9. Using a constructed wetland for non-point source pollution control and river water quality purification: a case study in Taiwan.

    Science.gov (United States)

    Wu, C Y; Kao, C M; Lin, C E; Chen, C W; Lai, Y C

    2010-01-01

    The Kaoping River Rail Bridge Constructed Wetland, which was commissioned in 2004, is one of the largest constructed wetlands in Taiwan. This multi-function wetland has been designed for the purposes of non-point source (NPS) pollutant removal, wastewater treatment, wildlife habitat, recreation, and education. The major influents of this wetland came from the local drainage trench containing domestic, agricultural, and industrial wastewaters, and effluents from the wastewater treatment plant of a paper mill. Based on the quarterly investigation results from 2007 to 2009, more than 96% of total coliforms (TC), 48% of biochemical oxygen demand (BOD), and 40% of nutrients (e.g. total nitrogen, total phosphorus) were removed via the constructed wetland system. Thus, the wetland system has a significant effect on water quality improvement and is capable of removing most of the pollutants from the local drainage system before they are discharged into the downgradient water body. Other accomplishments of this constructed wetland system include the following: providing more green areas along the riversides, offering more water assessable eco-ponds and eco-gardens for the public, and rehabilitating the natural ecosystem. The Kaoping River Rail Bridge Constructed Wetland has become one of the most successful multi-function constructed wetlands in Taiwan. The experience obtained from this study will be helpful in designing similar natural treatment systems for river water quality improvement and wastewater treatment.

  10. Non-Point Source Pollutant Load Variation in Rapid Urbanization Areas by Remote Sensing, Gis and the L-THIA Model: A Case in Bao'an District, Shenzhen, China

    Science.gov (United States)

    Li, Tianhong; Bai, Fengjiao; Han, Peng; Zhang, Yuanyan

    2016-11-01

    Urban sprawl is a major driving force that alters local and regional hydrology and increases non-point source pollution. Using the Bao'an District in Shenzhen, China, a typical rapid urbanization area, as the study area and land-use change maps from 1988 to 2014 that were obtained by remote sensing, the contributions of different land-use types to NPS pollutant production were assessed with a localized long-term hydrologic impact assessment (L-THIA) model. The results show that the non-point source pollution load changed significantly both in terms of magnitude and spatial distribution. The loads of chemical oxygen demand, total suspended substances, total nitrogen and total phosphorus were affected by the interactions between event mean concentration and the magnitude of changes in land-use acreages and the spatial distribution. From 1988 to 2014, the loads of chemical oxygen demand, suspended substances and total phosphorus showed clearly increasing trends with rates of 132.48 %, 32.52 % and 38.76 %, respectively, while the load of total nitrogen decreased by 71.52 %. The immigrant population ratio was selected as an indicator to represent the level of rapid urbanization and industrialization in the study area, and a comparison analysis of the indicator with the four non-point source loads demonstrated that the chemical oxygen demand, total phosphorus and total nitrogen loads are linearly related to the immigrant population ratio. The results provide useful information for environmental improvement and city management in the study area.

  11. The Non-point Source Pollution Effects of Pesticides Based on the Survey of 340 Farmers in Chongqing City

    Institute of Scientific and Technical Information of China (English)

    Lianchao; YU; Limeng; GU; Qian; BI

    2015-01-01

    Using the survey data on 340 farmers in Chongqing City,this paper performs an empirical analysis of the factors influencing the non-point source pollution of pesticides. The results show that the older householders will apply more pesticides,which may be due to the weak physical strength and weak ability to accept the concept of advanced cultivation; the householders with high level of education will choose to use less pesticides; the pesticide application rate is negatively correlated with whether farmers have participated in agricultural technology training,that is,the farmers having participated in agricultural technology training have stronger ability to scientifically apply pesticides,and in-depth understanding of advanced agricultural production technology and positive and negative effects of pesticides,so they often choose to reduce the application rate of pesticide; the cognitive factor on the role of pesticides in better promoting the growth of crops is significant,which requires the government and relevant departments to carry out concrete publicity of effectiveness and negative impact of different pesticides during the popularization of agricultural science knowledge,to prompt farmers to have a systematic and in-depth understanding of the agricultural nonpoint source pollution caused by pesticides.

  12. Export of non-point source suspended sediment, nitrogen, and phosphorus from sloping highland agricultural fields in the East Asian monsoon region.

    Science.gov (United States)

    Reza, Arif; Eum, Jaesung; Jung, Sungmin; Choi, Youngsoon; Owen, Jeffrey S; Kim, Bomchul

    2016-12-01

    Excess sediment and nutrient export from agricultural fields with steep slopes is a major concern linked to surface water quality in Korea. In this study, the export of suspended sediment (SS), total nitrogen (TN), and total phosphorus (TP) and their event mean concentrations (EMCs) in surface runoff from a highland mixed land use (61% forested, 30% cropped, 9% other) watershed were quantified. In 2007, the Korean Ministry of Environment (MoE) declared the study area as a priority region for non-point source (NPS) pollution management and initiated various best management practices (BMPs) in the study watershed. SS, TN, and TP concentrations in Mandae Stream were monitored for 5 years (2009-2013) to evaluate the effectiveness of BMPs. Average EMCs for SS, TN, and TP were as high as 986, 3.4 and 0.8 mg/L, respectively. The agricultural export coefficients of agricultural land in the study watershed for SS, TN, and TP were 5611, 171, and 6.83 kg/ha/year, respectively. A comparison with results from other studies shows that both EMCs and agricultural export coefficients in the study watershed were much higher than most of the results reported for watersheds in other regions. The results show that sediment and nutrient export from intensive agriculture areas with steep slopes continue to be a major concern for the downstream reservoir, Lake Soyang. Remedial strategies should be directed towards controlling sources of SS, TN, and TP to improve downstream water quality in sloping highland agricultural areas in Korea.

  13. A distributed non-point source pollution model:calibration and validation in the Yellow River Basin

    Institute of Scientific and Technical Information of China (English)

    HAO Fang-bua; ZHANG Xue-song; YANG Zhi-feng

    2004-01-01

    The applicability of a non-point source pollution model-SWAT(soil and water assessment tools) in a large river basin with high sediment runoff modulus(770 t/km2 in the Yellow River) was examined. The basic database,which includes DEM, soil and landuse map, weather data, and land management data, was established for the study area using GIS. A two-stage "Brute Force" optimization method was used to calibrate the parameters with the observed monthly flow and sediment data from 1992 to 1997. In the process of calibration automated digital filter technique was used to separate direct runoff and base flow. The direct runoff was firstly calibrated, and the base flow, then the total runoff was matched. The sediment yield was calibrated to match well. Keeping input parameters set during the calibration process unchanged, the model was validated with 1998-1999's observed monthly flow and sediment. The evaluation coefficients for simulated and observed flow and sediment showed that SWAT was successfully applied in the study area: relative error was within 20%, coefficient of determination and Nash-Suttcliffe simulation efficiency were all equal to or above 0.70 during calibration and validation period.

  14. Farmers’ Willingness to Pay( WTP ) for Reducing Agricultural Non-point Source Pollution: Based on the Empirical Analysis of 453 Farmers

    Institute of Scientific and Technical Information of China (English)

    Qian; BI; Huaiye; WANG; Yongyan; ZUO

    2014-01-01

    With the rapid development of modern agriculture,agricultural non-point source pollution becomes increasingly serious in China,improving farmers’ environmental protection consciousness plays a very important role in the reduction of agricultural non-point source pollution,and the WTP of Farmers for controlling agricultural non-point source pollution strongly reflects the strength of their environmental protection consciousness. Therefore,this investigation and study choose rural areas of five counties from Chongqing city and Zhejiang province as our sample,respectively make interview survey with the WTP(money or voluntary work) of farmers for improving local water and soil quality. Based on the statistical analysis of survey data,this study also takes empirical test and analysis with the influence factors on the WTP of farmers for reducing agricultural non-point source pollution. The analysis and conclusions of this research provides the supports in theory and practice for optimizing farmers’ behavior,promoting the management of agricultural non-point source pollution and implementing new rural construction goal.

  15. 非点源污染负荷模型的研究进展%Research Progress of Non-point Source Pollution Models in Water Environment

    Institute of Scientific and Technical Information of China (English)

    姚瑞华; 王东; 赵越; 张晶

    2012-01-01

    Based on non-point source load model research at home and abroad, in accordance of the agriculture, urban and mixed non-point source models, the main conditions for the application model were summarized, and the development of non-point source model was prospected.%基于国内外非点源负荷污染模型的研究现状,针对农业、城市和混合三种类型非点源模型,总结并梳理了主要模型的适用条件,并对非点源模型的发展趋势进行了展望.

  16. Estimation of contribution from non-point sources to perfluorinated surfactants in a river by using boron as a wastewater tracer.

    Science.gov (United States)

    Nishikoori, Hiroshi; Murakami, Michio; Sakai, Hiroshi; Oguma, Kumiko; Takada, Hideshige; Takizawa, Satoshi

    2011-08-01

    The contribution of non-point sources to perfluorinated surfactants (PFSs) in a river was evaluated by estimating their fluxes and by using boron (B) as a tracer. The utility of PFSs/B as an indicator for evaluating the impact of non-point sources was demonstrated. River water samples were collected from the Iruma River, upstream of the intake of drinking water treatment plants in Tokyo, during dry weather and wet weather, and 13 PFSs, dissolved organic carbon (DOC), total nitrogen (TN), and B were analyzed. Perfluorohexane sulfonate (PFHxS), perfluorooctane sulfonate (PFOS), perfluoroheptanoate (PFHpA), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), perfluoroundecanoate (PFUA), and perfluorododecanoate (PFDoDA) were detected on all sampling dates. The concentrations and fluxes of perfluorocarboxylates (PFCAs, e.g. PFOA and PFNA) were higher during wet weather, but those of perfluoroalkyl sulfonates (PFASs, e.g. PFHxS and PFOS) were not. The wet/dry ratios of PFSs/B (ratios of PFSs/B during wet weather to those during dry weather) agreed well with those of PFS fluxes (ratios of PFS fluxes during wet weather to those during dry weather), indicating that PFSs/B is useful for evaluating the contribution from non-point sources to PFSs in rivers. The wet/dry ratios of PFOA and PFNA were higher than those of other PFSs, DOC, and TN, showing that non-point sources contributed greatly to PFOA and PFNA in the water. This is the first study to use B as a wastewater tracer to estimate the contribution of non-point sources to PFSs in a river.

  17. Reduction rate of nitrogen and phosphorus non-point source pollution under different riparian buffer restoring strategies%不同河岸带修复策略对氮磷非点源污染的净化作用

    Institute of Scientific and Technical Information of China (English)

    赵鹏; 胡艳芳; 林峻宇

    2015-01-01

    This study was conducted along Liuxi River watershed, a main branch of Beijiang River, in Guangdong Province. Based on the non-point source (NPS) pollution and riparian buffer’s reduction in current situations of the Liuxi River Basin, the differences of NPS pollution reduction improvement of different restoration strategies were investigated by scenario simulations. In order to compare the efficiency of different restoration strategies, the relationships between the improvement of NPS reduction rate and the increase of buffer area were interpreted by benefit-cost index. The results showed the NPS reduction rate performed remarkable spatial variation. Each scenario had improved the NPS reduction rates to varying degrees (TN: 23.36%~30.72%; TP: 27.19%~39.86%). The relationships between buffer areas and NPS reduction rate of each scenario were fitted well by the logarithmic function (P<0.05). The strategy with restoring the sub-basins of prior riparian buffer restoration, which integrated NPS loading and riparian buffers conditions, could reach the best benefit-cost index (1.19%).%选择北江的重要支流流溪河流域为研究对象,基于现有河岸带对非点源污染削减作用的模拟结果,采用情景分析法,预测不同河岸带修复策略对非点源污染的削减作用,使用效益-成本指数表征 TN 和 TP 削减率提高幅度与增加河岸带面积的关系,比较不同河岸带修复策略的效率.结果表明:河岸带对TN和TP的削减能力具有较大的空间差异性.各修复情景都在不同程度上提高了河岸带对TN和TP的削减率(TN:23.36%~30.72%;TP:27.19%~39.86%),河岸带增加总面积与削减率呈现对数增长关系(P<0.05).选择综合考虑了流域非点源负荷量以及河岸带状况的优先修复子流域进行河岸带修复时,效益-成本指数达最高为1.19%.

  18. Non-Point Source Pollutant Load Variation in Rapid Urbanization Areas by Remote Sensing, Gis and the L-THIA Model: A Case in Bao'an District, Shenzhen, China.

    Science.gov (United States)

    Li, Tianhong; Bai, Fengjiao; Han, Peng; Zhang, Yuanyan

    2016-11-01

    Urban sprawl is a major driving force that alters local and regional hydrology and increases non-point source pollution. Using the Bao'an District in Shenzhen, China, a typical rapid urbanization area, as the study area and land-use change maps from 1988 to 2014 that were obtained by remote sensing, the contributions of different land-use types to NPS pollutant production were assessed with a localized long-term hydrologic impact assessment (L-THIA) model. The results show that the non-point source pollution load changed significantly both in terms of magnitude and spatial distribution. The loads of chemical oxygen demand, total suspended substances, total nitrogen and total phosphorus were affected by the interactions between event mean concentration and the magnitude of changes in land-use acreages and the spatial distribution. From 1988 to 2014, the loads of chemical oxygen demand, suspended substances and total phosphorus showed clearly increasing trends with rates of 132.48 %, 32.52 % and 38.76 %, respectively, while the load of total nitrogen decreased by 71.52 %. The immigrant population ratio was selected as an indicator to represent the level of rapid urbanization and industrialization in the study area, and a comparison analysis of the indicator with the four non-point source loads demonstrated that the chemical oxygen demand, total phosphorus and total nitrogen loads are linearly related to the immigrant population ratio. The results provide useful information for environmental improvement and city management in the study area.

  19. Agricultural Non-point Source Pollution and Countermeasures in Yunnan%云南省农业面源污染及防治对策

    Institute of Scientific and Technical Information of China (English)

    邱成

    2014-01-01

    云南省主要农业环境污染问题包括:化肥使用量不断增加;地膜使用量及其覆盖面积不断增加;农药使用量不断增加;畜禽粪便污染日益加重。分析了问题产生的原因,提出了防治农业污染的建议。%Environmental problems are emerging due to agricultural non -point source pollution in Yunnan.The most serious problems are as follows:an increasing usage of chemical fertilizers and plastics in farm field,a rising release of pesticide,and a growing amount of waste from livestock and poultry.Agricultural non-point sources in Yunnan were identified.Suggestions were put forward to control agricultural pollution.

  20. 农业非点源污染研究进展和趋势%The Progress and Trends of Agricultural Non-point Source Pollution Research

    Institute of Scientific and Technical Information of China (English)

    李丽华; 李强坤

    2014-01-01

    根据国内外农业非点源污染研究现状,本文在探讨农业非点源污染内涵及其特征的基础上,简要总结了农业非点源污染负荷的估算模型,列举区域农业非点源污染风险评估的手段和方法,从不同角度归纳了农业非点源污染的控制技术,并提出了近期农业非点源污染急需研究的热点和趋势,以期为进一步的农业非点源污染管理和控制提供参考。%According to the current research on agricultural non-point source pollution at home and abroad, the connotation and feature of a-gricultural non-point source pollution were explored in this paper, and then the estimating model of pollution load was concluded briefly. Meanwhile, the paper also listed the means and methods of risk assessment of regional agricultural non-point source pollution and summed up the control technologies from different angles. Finally, the recent much-needed research hotspots and trends were put forward in order to provide reference for further management and control of agricultural non-point source pollution.

  1. Non-point source analysis of a railway bridge area using statistical method: case study of a concrete road-bed.

    Science.gov (United States)

    Gil, Kyungik; Im, Jiyeol

    2014-06-01

    In an effort to protect the quality of the water system, interest in non-point source pollution is increasing. Recently, studies of non-point sources pollution are continuing in relation to various land-use areas, but such studies have not been fully conducted in railway facility sites. Using monitoring data of railway bridge area with concrete road-bed, the runoff characteristics, pollutant unit loads, and first flush criteria were assessed. Railway bridge area with concrete road-bed typically show the first flush effect, and the pollutant unit load was determined to be higher than other public facilities areas. Further, the first flush criteria show an effective rainfall amount of 7 mm. In other words, from the runoff of railway facilities, considerable amounts of non-point source pollutants are occurred, indicating the need to create best management practices which are adequate for railway facility sites. Copyright © 2014 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

  2. Multi-angle Indicators System of Non-point Pollution Source Assessment in Rural Areas: A Case Study Near Taihu Lake

    Science.gov (United States)

    Huang, Lei; Ban, Jie; Han, Yu Ting; Yang, Jie; Bi, Jun

    2013-04-01

    This study aims to identify key environmental risk sources contributing to water eutrophication and to suggest certain risk management strategies for rural areas. The multi-angle indicators included in the risk source assessment system were non-point source pollution, deficient waste treatment, and public awareness of environmental risk, which combined psychometric paradigm methods, the contingent valuation method, and personal interviews to describe the environmental sensitivity of local residents. Total risk values of different villages near Taihu Lake were calculated in the case study, which resulted in a geographic risk map showing which village was the critical risk source of Taihu eutrophication. The increased application of phosphorus (P) and nitrogen (N), loss vulnerability of pollutant, and a lack of environmental risk awareness led to more serious non-point pollution, especially in rural China. Interesting results revealed by the quotient between the scores of objective risk sources and subjective risk sources showed what should be improved for each study village. More environmental investments, control of agricultural activities, and promotion of environmental education are critical considerations for rural environmental management. These findings are helpful for developing targeted and effective risk management strategies in rural areas.

  3. Detection of spatial fluctuations of non-point source fecal pollution in coral reef surrounding waters in southwestern Puerto Rico using PCR-based assays.

    Science.gov (United States)

    Bonkosky, M; Hernández-Delgado, E A; Sandoz, B; Robledo, I E; Norat-Ramírez, J; Mattei, H

    2009-01-01

    Human fecal contamination of coral reefs is a major cause of concern. Conventional methods used to monitor microbial water quality cannot be used to discriminate between different fecal pollution sources. Fecal coliforms, enterococci, and human-specific Bacteroides (HF183, HF134), general Bacteroides-Prevotella (GB32), and Clostridium coccoides group (CP) 16S rDNA PCR assays were used to test for the presence of non-point source fecal contamination across the southwestern Puerto Rico shelf. Inshore waters were highly turbid, consistently receiving fecal pollution from variable sources, and showing the highest frequency of positive molecular marker signals. Signals were also detected at offshore waters in compliance with existing microbiological quality regulations. Phylogenetic analysis showed that most isolates were of human fecal origin. The geographic extent of non-point source fecal pollution was large and impacted extensive coral reef systems. This could have deleterious long-term impacts on public health, local fisheries and in tourism potential if not adequately addressed.

  4. Screening and identification of early warning algal species for metal contamination in fresh water bodies polluted from point and non-point sources.

    Science.gov (United States)

    Rai, U N; Dubey, Smita; Shukla, O P; Dwivedi, S; Tripathi, R D

    2008-09-01

    The water bodies of Lucknow, Unnao and Kanpur (U.P.), India polluted through various point and non point sources were found to be either eutrophic or oligotrophic in nature. These water bodies supported a great number of algal diversity, which varied seasonally depending upon the physico-chemical properties of water. Further, the water bodies polluted through non point sources supports diverse algal species, while the water bodies polluted through point sources supports growth of tolerant blue green algae. High biomass producing algal species growing in these water bodies have accumulated significant amount of metals in their tissues. Maximum amount of Fe was found accumulated by species of Oedogonium sp. II (20,523.00 microg g(-1) dw) and Spirogyra sp. I (4,520.00 microg g(-1) dw), while maximum Chromium (Cr) was found accumulated in Phormedium bohneri (2,109.00 microg g(-1) dw) followed by Oscillatoria nigra (1,957.88 microg g(-1) dw) and Oedogonium sp. I (156.00 microg g(-1) dw) and Ni in Ulothrix sp. (495.00 microg g(-1) dw). Results showed that some of these forms growing in polluted environment and accumulating high amounts of toxic metals may be used as bioindicator species, however, their performance in metal contaminated water under different ecological niche is to be ascertained.

  5. Study on agricultural structure and non-point source pollution: a case in Dapu Town of Yixing City

    Institute of Scientific and Technical Information of China (English)

    Jiang Dongmei; Wang Xiyuan; Liu Minghui; Lu Genfa

    2006-01-01

    The water body of Taihu Lake has been eutrophicated because of area-source pollution. 83% of the total nitrogen and 84% of the total phosphorus of the pollutant that have washed into Taihu Lake originated from the fertilizer of crop land, rural animal husbandry and living sewage and rubbish in rural area. The goal of adjusting agricultural structure is to improve agricultural development, and to increase the peasants income by planting non-grain crop,centralizing animal husbandry, and intensifying aquaculture, etc, It is necessary to research on the influences of agriculture industrial structure on area-source pollution, This paper studies a case of Dapu Town in Yixing City, which is a typical drainage place beside Taihu Lake. On the basis of the analysis on the status quo of area-source pollution and agriculture industrial structure in Dapu Town, the conflicts between them are discussed. Non-grain crop production with a great deal of fertilizer and developing aquaculture with a great deal of organic pollutant, which are directly discharged,make area-source pollution more serious and accelerate the eutrophication in Taihu Lake. This paper suggests taking corresponding technological measures and policies, which have been tested in Dapu Town and demonstrated in Taihu Lake area.

  6. Synthesis of lignin-based copolymer LSAA and the application on controlling non-point source pollution (NPS) resulted from surface runoff

    Institute of Scientific and Technical Information of China (English)

    LIU Chen; WU Guangxia; MU Huanzhen; YUAN Zonghuan; TANG Lianyi; LIN Xiangwei

    2008-01-01

    In this article, alkali lignin separated from paper pulp waste was grafted into a novel copolymer LSAA (a copolymer of lignin, starch, acrylamide and acrylic acid). Its practical application effect and environmental safety were studied. The results of field simulation experiment indicated that the application of LSAA significantly affected the output of the runoff and pollutants. The runoff quantity was decreased by 16.67%-47.00% and the loads of total suspended solids (TSS), chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were reduced by 17.78%-62.14%, 26.32%-59.91%, 26.67%-42.86%, and 22.18%-52.78%, respectively. Tests on its environmental safety showed that LSAA did no harm the soil. Compared with polyacrylamide (PAM), a dominant product in this field, LSAA exhibited similar effects and cheap cost. Thus, this study not only created a new product for controlling runoff water quality but also offered a beneficial application for industrial paper waste.

  7. A dual-inexact fuzzy stochastic model for water resources management and non-point source pollution mitigation under multiple uncertainties

    Science.gov (United States)

    Dong, C.; Tan, Q.; Huang, G.-H.; Cai, Y.-P.

    2014-05-01

    In this research, a dual-inexact fuzzy stochastic programming (DIFSP) method was developed for supporting the planning of water and farmland use management system considering the non-point source pollution mitigation under uncertainty. The random boundary interval (RBI) was incorporated into DIFSP through integrating fuzzy linear programming (FLP) and chance-constrained programming (CCP) approaches within an interval linear programming (ILP) framework. This developed method could effectively tackle the uncertainties expressed as intervals and fuzzy sets. Moreover, the lower and upper bounds of RBI are continuous random variables, and the correlation existing between the lower and upper bounds can be tackled in RBI through the joint probability distribution function. And thus the subjectivity of decision making is greatly reduced, enhancing the stability and robustness of obtained solutions. The proposed method was then applied to solve a water and farmland use planning model (WFUPM) with non-point source pollution mitigation. The generated results could provide decision makers with detailed water supply-demand schemes involving diversified water-related activities under preferred satisfaction degrees. These useful solutions could allow more in-depth analyses of the trade-offs between humans and environment, as well as those between system optimality and reliability. In addition, comparative analyses on the solutions obtained from ICCP (Interval chance-constraints programming) and DIFSP demonstrated the higher application of this developed approach for supporting the water and farmland use system planning.

  8. Trends of nitrogen and phosphorus input into Lake Neusiedl from wastewater treatment plants and non-point sources

    Science.gov (United States)

    Kinner, Paul; Heiss, Gerhard; Soja, Gerhard

    2013-04-01

    nitrogen load amounted to 304 tons in 2010, compared to 47 tons in 2001. In the period 1992-2010 the nitrogen load caused by diffuse sources was 4.3 times higher than the point source nitrogen load (2 wastewater treatment plants). The proportion of total discharge, of inorganic nitrogen load and of phosphorus load caused by the two wastewater treatment plants depended on the discharge rate of the river Wulka (monitoring station Schützen). In 2001 (low precipitation year: 578 mm annual sum) point sources contributed about 47% of the discharge, 51% of the nitrogen load and 65% of the phosphorus load of the river Wulka. In 2010 (high precipitation year: 945 mm annual sum) point sources contributed 25% of the discharge, 11% of the nitrogen load and 31% of the total phosphorus load. In the period 1992 to 2010 the inorganic nitrogen load caused by surface water (Wulka, WWTP, creeks and channels) varied from 65 t/a to 675 t/a (mean:233 t/a).

  9. Identification of new psychoactive substances (NPS) using handheld Raman spectroscopy employing both 785 and 1064nm laser sources.

    Science.gov (United States)

    Guirguis, Amira; Girotto, Sarah; Berti, Benedetta; Stair, Jacqueline L

    2017-02-04

    The chemical identification of new psychoactive substances (NPS) in the field is challenging due not only to the plethora of substances available, but also as a result of the chemical complexity of products and the chemical similarity of NPS analogues. In this study, handheld Raman spectroscopy and the use of two excitation wavelengths, 785 and 1064nm, were evaluated for the identification of 60 NPS products. The products contained a range of NPS from classes including the aminoindanes, arylalkylamines, benzodiazepines, and piperidines & pyrrolidines. Identification was initially assessed using the instruments' in built algorithm (i.e., % HQI) and then further by visual inspection of the Raman spectra. Confirmatory analysis was preformed using gas chromatography mass spectrometry. For the 60 diverse products, an NPS was successfully identified via the algorithm in 11 products (18%) using the 785nm source and 29 products (48%) using the 1064nm source. Evaluation of the Raman spectra showed that increasing the excitation wavelength from 785 to 1064nm improved this 'first pass' identification primarily due to a significant reduction in fluorescence, which increased S/N of the characteristic peaks of the substance identified. True positive correlations between internet products and NPS signatures ranged from 57.0 to 91.3% HQI with typical RSDs<10%. Tablet formulations and branded products were particularly challenging as a result of low NPS concentration and high chemical complexity, respectively. This study demonstrates the advantage of using a 1064nm source with handheld Raman spectroscopy for improved 'first pass' NPS identification when minimal spectral processing is required, such as when working in field. Future investigations will focus on the use of mixture algorithms, effect of NPS concentration, and further improvement of spectral libraries.

  10. Study on Control Countermeasures of Agricultural Non-point Source Pollution in Lakeside Belt of Poyang Lake——Taking Duchang Section in the Lower Reaches of Poyang Lake as Example

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective] The aim was to study the control countermeasures of agricultural non-point source pollution in lakeside belt of Poyang Lake.[Method] The current situation of water quality of Poyang Lake was analyzed firstly,then the causes of agricultural non-point source pollution in Duchang section of Poyang Lake were studied,finally corresponding control countermeasures were put forward.[Result] Agricultural non-point source pollution in Duchang section of Poyang Lake was mainly related to the rapid developm...

  11. Assessment of Non-Point Source Total Phosphorus Pollution from Different Land Use and Soil Types in a Mid-High Latitude Region of China

    Directory of Open Access Journals (Sweden)

    Zhiwei Wang

    2016-11-01

    Full Text Available The transport characteristics of phosphorus in soil and the assessment of its environmental risk have become hot topics in the environmental and agricultural fields. The Sanjiang Plain is an important grain production base in China, and it is characterised by serious land use change caused by large-scale agricultural exploitation. Agricultural inputs and tillage management have destroyed the soil nutrient balance formed over long-term conditions. There are few studies on non-point source phosphorus pollution in the Sanjiang Plain, which is the largest swampy low plain in a mid-high-latitude region in China. Most studies have focused on the water quality of rivers in marsh areas, or the export mechanism of phosphorus from specific land uses. They were conducted using experimental methods or empirical models, and need further development towards mechanism models and the macro-scale. The question is how to find a way to couple processes in phosphorus cycling and a distributed hydrological model considering local hydrological features. In this study, we report an attempt to use a distributed phosphorus transport model to analyse non-point source total phosphorus pollution from different land uses and soil types on the Sanjiang Plain. The total phosphorus concentration generally shows an annually increasing trend in the study area. The total phosphorus load intensity is heterogeneous in different land use types and different soil types. The average total phosphorus load intensity of different land use types can be ranked in descending order from paddy field, dry land, wetlands, grassland, and forestland. The average total phosphorus load intensity of different soil types can be ranked in descending order: paddy soil, bog soil, planosol, meadow soil, black soil, and dark brown earth. The dry land and paddy fields account for the majority of total phosphorus load in the study area. This is mainly caused by extensive use of phosphate fertilizer on the

  12. Combination system of full-scale constructed wetlands and wetland paddy fields to remove nitrogen and phosphorus from rural unregulated non-point sources.

    Science.gov (United States)

    Sun, Haijun; Zhang, Hailin; Yu, Zhimin; Wu, Jiasen; Jiang, Peikun; Yuan, Xiaoyan; Shi, Weiming

    2013-12-01

    Constructed wetlands (CWs) have been used effectively to remove nitrogen (N) and phosphorus (P) from non-point sources. Effluents of some CWs were, however, still with high N and P concentrations and remained to be pollution sources. Widely distributed paddy fields can be exploited to alleviate this concern. We were the first to investigate a combination system of three-level CWs with wetland paddy fields in a full scale to remove N and P from rural unregulated non-point sources. The removal efficiencies (REs) of CWs reached 57.3 % (37.4-75.1 %) for N and 76.3 % (62.0-98.4 %) for P. The CWs retained about 1,278 kg N ha(-1) year(-1) and 121 kg P ha(-1) year(-1). There was a notable seasonal change in REs of N and P, and the REs were different in different processing components of CWs. The removal rates of wetland paddy fields adopt "zero-drainage" water management according to local rainfall forecast and physiological water demand of crop growth reached 93.2 kg N ha(-1) year(-1) and 5.4 kg P ha(-1) year(-1). The rice season had higher potential in removing N and P than that in the wheat season. The whole combined system (0.56 ha CWs and 5.5 ha wetland paddy fields) removed 1,790 kg N year(-1) and 151 kg P year(-1), which were higher than those from CWs functioned alone. However, another 4.7-ha paddy fields were needed to fully remove the N and P in the effluents of CWs. The combination of CWs and paddy fields proved to be a more efficient nutrient removal system.

  13. Linking monitoring and modelling for river basin management:Danish experience with combating nutrient loadings to the aquatic environment from point and non-point sources

    Institute of Scientific and Technical Information of China (English)

    KRONVANG; Brian; WINDOLF; JФrgen; GRANT; Ruth; ANDERSEN; Hans; E; THODSEN; Hans; OVESEN; Niels; B; LARSEN; SФren; E

    2009-01-01

    Nationwide monitoring of the aquatic environment was initiated in 1988 in Denmark as a means to follow the outcome of the Action Plans for nutrient pollution of the aquatic environment. Five Action Plans have been adopted by the Danish Parliament since 1985 and the nationwide monitoring programme can be used to quantify the outcome as shown by reductions in nutrient discharges from both point and non-point sources. Moreover, the empirical experience gathered from nearly 20 years of monitoring is assisting the development and calibration of models for simulation of nitrogen leaching, nitrogen removal in groundwater and surface waters and the establishment of a P-index all covering the entire land area of Denmark.

  14. Linking monitoring and modelling for river basin man-agement: Danish experience with combating nutrient loadings to the aquatic environment from point and non-point sources

    Institute of Scientific and Technical Information of China (English)

    KRONVANG Brian; WINDOLF J(φ)rgen; GRANT Ruth; ANDERSEN Hans E; THODSEN Hans; OVESEN Niels B; LARSEN S(φ)ren E

    2009-01-01

    Nationwide monitoring of the aquatic environment was initiated in 1988 in Denmark as a means to fol-low the outcome of the Action Plans for nutrient pollution of the aquatic environment.Five Action Plans have been adopted by the Danish Parliament since 1985 and the nationwide monitoring programme can be used to quantify the outcome as shown by reductions in nutrient discharges from both point and non-point sources.Moreover, the empirical experience gathered from nearly 20 years of monitoring is assisting the development and calibration of models for simulation of nitrogen leaching, nitrogen re-moval in groundwater and surface waters and the establishment of a P-index all covering the entire land area of Denmark.

  15. [Analysis on nitrogen and phosphorus loading of non-point sources in Shiqiao river watershed based on L-THIA model].

    Science.gov (United States)

    Li, Kai; Zeng, Fan-Tang; Fang, Huai-Yang; Lin, Shu

    2013-11-01

    Based on the Long-term Hydrological Impact Assessment (L-THIA) model, the effect of land use and rainfall change on nitrogen and phosphorus loading of non-point sources in Shiqiao river watershed was analyzed. The parameters in L-THIA model were revised according to the data recorded in the scene of runoff plots, which were set up in the watershed. The results showed that the distribution of areas with high pollution load was mainly concentrated in agricultural land and urban land. Agricultural land was the biggest contributor to nitrogen and phosphorus load. From 1995 to 2010, the load of major pollutants, namely TN and TP, showed an obviously increasing trend with increase rates of 17.91% and 25.30%, respectively. With the urbanization in the watershed, urban land increased rapidly and its area proportion reached 43.94%. The contribution of urban land to nitrogen and phosphorus load was over 40% in 2010. This was the main reason why pollution load still increased obviously while the agricultural land decreased greatly in the past 15 years. The rainfall occurred in the watershed was mainly concentrated in the flood season, so the nitrogen and phosphorus load of the flood season was far higher than that of the non-flood season and the proportion accounting for the whole year was over 85%. Pearson regression analysis between pollution load and the frequency of different patterns of rainfall demonstrated that rainfall exceeding 20 mm in a day was the main rainfall type causing non-point source pollution.

  16. Impact of Point and Non-point Source Pollution on Coral Reef Ecosystems In Mamala Bay, Oahu, Hawaii based on Water Quality Measurements and Benthic Surveys in 1993-1994 (NODC Accession 0001172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The effects of both point and non-point sources of pollution on coral reef ecosystems in Mamala Bay were studied at three levels of biological organization; the...

  17. 河流污染的点源和非点源负荷分割研究%The Segmentation of the Point Source and Non-point Source Pollution Load of Rivers

    Institute of Scientific and Technical Information of China (English)

    乔继平; 代俊峰

    2015-01-01

    Non-point source pollution has become the major source of pollution of water environment .Point source pollution and non-point source pollution of river water environmental monitoring to distinguish sections is very necessary .This paper mainly intro‐duces the research progress at home and abroad about non point source pollution .This paper focuses on the pollution load partition of Nanliu River Basin in Guangxi Beibu Gulf Economic Zone .The segmentation of point source pollution and non-point source pollution of potassium permanganate index ,total phosphorus and ammonia nitrogen are made based on the hydrological estimation method and the digital filtering method .The hydrological estimation method results show that three indexes of non-point source pollution the to‐tal pollution load ratio is 0 .77 ,0 .74 and 0 .76 .And the digital filtering method results show that they are 0 .59 ,0 .64 and 0 .66 .Di‐viding two methods of non-point source is close to the result ,Nanliu River Basin is more serious .%非点源污染已经成为水环境的主要污染源,区分河流水环境监测断面的点源污染与非点源污染显得十分必要。以广西入海河流南流江为研究背景,采用数字滤波法和水文估算法进行点源与非点源污染负荷的分割。分析结果显示,基于水文估算法计算的2003-2011年多年平均高锰酸盐指数、总磷、氨氮的非点源污染负荷占总负荷的比例分别为0.77、0.74、0.76;采用数字滤波法计算的2003-2011年多年平均高锰酸盐指数、总磷、氨氮的非点源污染负荷占总负荷的比例分别为0.59、0.64、0.66。结果表明,两种方法分割的非点源结果的变幅趋势相同,南流江的非点源污染在总污染负荷中的比例较高。

  18. Nitrogen Non-Point Source Pollution Identification Based on ArcSWAT in Changle River%基于ArcSWAT模型的长乐江流域非点源氮素污染源识别和分析

    Institute of Scientific and Technical Information of China (English)

    邓欧平; 孙嗣旸; 吕军

    2013-01-01

    The ArcSWAT (Soil and Water Assessment Tool) model was adopted for Non-point source ( NPS) nitrogen pollution modeling and nitrogen source apportionment for the Changle River watershed, a typical agricultural watershed in Southeast China. Water quality and hydrological parameters were monitored, and the watershed natural conditions (including soil, climate, land use, etc) and pollution sources information were also investigated and collected for SWAT database. The ArcSWAT model was established in the Changle River after the calibrating and validating procedures of the model parameters. Based on the validated SWAT model, the contributions of different nitrogen sources to river TN loading were quantified, and spatial-temporal distributions of NPS nitrogen export to rivers were addressed. The results showed that in the Changle River watershed, Nitrogen fertilizer, nitrogen air deposition and nitrogen soil pool were the prominent pollution sources, which contributed 35% , 32% and 25% to the river TN loading, respectively. There were spatial-temporal variations in the critical sources for NPS TN export to the river. Natural sources, such as soil nitrogen pool and atmospheric nitrogen deposition, should be targeted as the critical sources for river TN pollution during the rainy seasons. Chemical nitrogen fertilizer application should be targeted as the critical sources for river TN pollution during the crop growing season. Chemical nitrogen fertilizer application, soil nitrogen pool and atmospheric nitrogen deposition were the main sources for TN exported from the garden plot, forest and residential land, respectively. However, they were the main sources for TN exported both from the upland and paddy field. These results revealed that NPS pollution controlling rules should focus on the spatio-temporal distribution of NPS pollution sources.%本研究以我国东南沿海地区的典型农业流域——长乐江流域为对象,通过实地调查、数据收集和分析,

  19. {sup 37}Cl, {sup 15}N, {sup 13}C isotopic analysis of common agro-chemicals for identifying non-point source agricultural contaminants

    Energy Technology Data Exchange (ETDEWEB)

    Annable, W.K. [Department of Civil and Environmental Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1 (Canada)]. E-mail: wkannabl@uwaterloo.ca; Frape, S.K. [Department of Earth Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1 (Canada); Shouakar-Stash, O. [Department of Earth Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1 (Canada); Shanoff, T. [Department of Earth Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1 (Canada); Drimmie, R.J. [Department of Earth Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1 (Canada); Harvey, F.E. [School of Natural Resources, University of Nebraska, Lincoln, NE 68588-0517 (United States)

    2007-07-15

    The isotopic compositions of commercially available herbicides were analyzed to determine their respective {sup 15}N, {sup 13}C and {sup 37}Cl signatures for the purposes of developing a discrete tool for tracing and identifying non-point source contaminants in agricultural watersheds. Findings demonstrate that of the agrochemicals evaluated, chlorine stable isotopes signatures range between {delta}{sup 37}Cl = -4.55 per mille and +3.40 per mille , whereas most naturally occurring chlorine stable isotopes signatures, including those of road salt, sewage sludge and fertilizers, vary in a narrow range about the Standard Mean Ocean Chloride (SMOC) between -2.00 per mille and +1.00 per mille . Nitrogen stable isotope values varied widely from {delta}{sup 15}N = -10.86 per mille to +1.44 per mille and carbon stable isotope analysis gave an observed range between {delta}{sup 13}C = -37.13 per mille and -21.35 per mille for the entire suite of agro-chemicals analyzed. When nitrogen, carbon and chlorine stable isotope analyses were compared in a cross-correlation analysis, statistically independent isotopic signatures exist suggesting a new potential tracer tool for identifying herbicides in the environment.

  20. Sensitivity analysis of non-point sources in a water quality model applied to a dammed low-flow-reach river.

    Science.gov (United States)

    Silva, Nayana G M; von Sperling, Marcos

    2008-01-01

    Downstream of Capim Branco I hydroelectric dam (Minas Gerais state, Brazil), there is the need of keeping a minimum flow of 7 m3/s. This low flow reach (LFR) has a length of 9 km. In order to raise the water level in the low flow reach, the construction of intermediate dikes along the river bed was decided. The LFR has a tributary that receives the discharge of treated wastewater. As part of this study, water quality of the low-flow reach was modelled, in order to gain insight into its possible behaviour under different scenarios (without and with intermediate dikes). QUAL2E equations were implemented in FORTRAN code. The model takes into account point-source pollution and diffuse pollution. Uncertainty analysis was performed, presenting probabilistic results and allowing identification of the more important coefficients in the LFR water-quality model. The simulated results indicate, in general, very good conditions for most of the water quality parameters The variables of more influence found in the sensitivity analysis were the conversion coefficients (without and with dikes), the initial conditions in the reach (without dikes), the non-point incremental contributions (without dikes) and the hydraulic characteristics of the reach (with dikes).

  1. 拉萨河流域非点源污染输出风险评估%Risk assessment of non-point source pollution export in Lasahe basin

    Institute of Scientific and Technical Information of China (English)

    方广玲; 香宝; 杜加强; 王宝良; 张立坤; 胡钰; 徐杰

    2015-01-01

    Identification of the high risk area of non-point source pollution has important practical significance to control non-point source pollution and improve the water environment quality. Lasahe River basin located in the Tibet Autonomous Region of China is important drinking-water source for Lhasa City, Linzhou County and Dangxiong County. Because the underdevelopment of industry and the less discharge of industrial pollutants in this area, non-point source pollution is the most important contributing factor for water pollution. Lhasa River basin is the region of agricultural production base with the densest population in the Tibet Autonomous Region. This study built the output risk model that includes rainfall, topography, and fertilization influence factor, identification the output risk region unit from basin non-point source pollution at all levels object. The risk probability of non-point source pollution was classed into five levels: lowest, lower, moderate, higher and highest. Data used in this study were mainly from remote sensing image, statistical yearbook, and the parameters in output risk model were collected from the literatures. The results showed that risk probability of non-point source pollution output in 1996 and 2010 was 50%and 46.3%respectively. 17.5%in 1996 and 12.6%in 2010 of the study area showed that risk probability of non-point source pollution was more than 70%. The risk probability of non-point source pollution was spatially heterogeneous, corresponding with the attributes of land use types. The areas with highest risk of non-point source pollution were concentrated in farmland, where agricultural activities strengthened, and concentrated in unused land with great ecological vulnerability and sensitivity to external interference. Areas with lowest and lower risk of non-point source pollution mainly distributed in grassland, which has relative stability and robustness. Our study also found that risk probability of non-point source pollution

  2. 植草沟技术在面源污染控制中的研究进展%Advance on Grassed Swales Technology in Non-point Source Pollution Control

    Institute of Scientific and Technical Information of China (English)

    王健; 尹炜; 叶闽; 雷阿林; 李思敏

    2011-01-01

    Current research advances of non-point source pollution, especially urban non-point source pollution were introduced. Research advances and applications of grassed swales technology in non-point source pollution control were expounded. Main problems and application prospects of grassed swales technology in actual engineering were discussed as well. Coupling of grassed swales technology and other non-point source pollution control technology are proposed to be considered as a development direction in urban non-point source pollution control.%文章介绍了面源污染,尤其是城市面源污染的现状及其控制技术研究进展,并论述了植草沟技术的国内外研究进展及应用现状,讨论了其在工程中需解决的问题及应用前景,提出了植草沟技术与其他面源污染控制单项技术有机耦合是城市面源污染控制的一个发展方向.

  3. The urban atmosphere as a non-point source for the transport of MTBE and other volatile organic compounds (VOCS) to shallow groundwater

    Science.gov (United States)

    Pankow, J.F.; Thomson, N.R.; Johnson, R.L.; Baehr, A.L.; Zogorski, J.S.

    1997-01-01

    all no- net recharge cases. The mechanism responsible for this effect was the dispersion acting on each downward infiltration event, and also on the ET-induced flow. The ability of MTBE to reach groundwater in cases 2-5 is taken as evidence of the potential importance of urban air as a non-point source for VOCs in shallow urban groundwater. Two subcases were run for both case 4 and case 5: subcase a (water and VOCs move with ET) and subcase b (water only moves with ET).Numerical simulations were conducted using a 1-D model domain set in medium sand to provide a test of whether methyl-tert-butyl ether (MTBE) and other atmospheric volatile organic compounds could move to shallow groundwater within the 10-15 y time frame over which MTBE was used in large amounts. The gasoline additive MTBE is of special interest because of its: current levels in some urban air; strong partitioning from air into water; resistance to degradation; use as an octane-booster since the 1970s; rapidly increasing use in the 1990s to reduce CO and O3 in urban air; and its frequent detection at low microgram per liter levels in shallow urban groundwater.

  4. Discussion on the Green Tax Stimulation Measure of Nitrogen Fertilizer Non-Point Source Pollution Control - Taking the Dongting Lake Area in China as a Case

    Institute of Scientific and Technical Information of China (English)

    XIANG Ping-an; ZHOU Yan; HUANG Huang; ZHENG Hua

    2007-01-01

    A study on designing the tax of nitrogen fertilizer can provide a new method for controlling nitrogen fertilizer non-point source pollution. The tax design of nitrogen fertilizer was discussed by utilizing the external theory and the demand elasticity theory. The results indicated that the coefficient of price elasticity of nitrogen fertilizer demand is -0.21, which instructed that the market demand is in lack of elasticity in the short period and the impact of nitrogen fertilizer manufacturers is subtle. The 11 counties (cities and boroughs) in the Dongting Lake area in China, where the farmland nitrogen application surpassed the average ecological fertilization dosage, is listed to the taxation scope of nitrogen fertilizer tax. The environment loss will reduce 0.07 hundred million RMB yuan and the revenue will increase 0.89 hundred million RMB yuan in the country after levying on nitrogen fertilizer. The loss, which was brought by the decreasing food supplies production, will be 0.58 hundred million RMB yuan and the net social benefit will be 0.38 hundred million RMB yuan following revenue collection. The variation scope of the increasing expenditure of farmers will range from 0.95 to 1.49%. The variation scope of the income of farmers will range from -8.41 to 6.44%. The 5 areas, Yunxi Borough, Junshan Borough, Hanshou County, Jinshi City, and Ziyang Borough, had an increase in food supplies production after the revenue collection. The environment loss will reduce 0.01 hundred million RMB yuan and the revenue will increase 0.16 hundred million RMB yuan in the country after levying on nitrogen fertilizer. The economic benefits, which was brought by the increasing cereals production, will be 0.67 hundred million RMB yuan and the net social benefit will be 0.84 hundred million RMB yuan after revenue collection. The variation scope of the increasing expenditure of farmers will range from 0.95 to 1.06%. The variation scope of the increasing income of farmers will

  5. Spatial and temporal variability of heavy metals in streams of the Flint Creek and Flint River Watersheds from non-point sources

    OpenAIRE

    I. Abdi; T. Tsegaye; Silitonga, M.; Tadesse, W

    2009-01-01

    Throughout the United States, non-point pollution is responsible for large quantities of heavy metals entering bodies of water. Pollution as a result of heavy metals can impact drinking water supplies, recreation, fisheries, and aquatic species. Presence of heavy metals such as lead (Pb), cadmium (Cd), and chromium (Cr), in surface water may pose great risks to human health as well as to aquatic animals. In order to understand water quality changes due to heavy metal element...

  6. Spatial and temporal variability of heavy metals in streams of the Flint Creek and Flint River Watersheds from non-point sources

    OpenAIRE

    Tadesse, W; Silitonga, M.; I. Abdi; T. Tsegaye

    2009-01-01

    Throughout the United States, non-point pollution is responsible for large quantities of heavy metals entering bodies of water. Pollution as a result of heavy metals can impact drinking water supplies, recreation, fisheries, and aquatic species. Presence of heavy metals such as lead (Pb), cadmium (Cd), and chromium (Cr), in surface water may pose great risks to human health as well as to aquatic animals. In order to understand water quality changes due to heavy metal elements and pH as a resu...

  7. Spatial Distribution Pattern of Agricultural Non-point Source Pollution in Jiangxi Province%江西省农业面源污染空间分布格局

    Institute of Scientific and Technical Information of China (English)

    张文东; 许仕; 庐俊

    2012-01-01

    The agricultural non-point source pollution is becoming an increasingly important eco-envjonrmental problem, il is difficult lo control due lo its dispersive and extensive characteristics. Jiangxi Province is still at ihe initial stage ai agricultural modernization, bul its agricultural non-point source pollution is exacerbating. According to the investigation on the pollution sources of planting, animal husbandry and aq-uaculture in Jiangs i Province, the ArcGIS software was adopted to analyze the spatial and digital features of agricultural non-point source pollution. As indicated by the results, the principal pollution source is animal husbandry, then followed by planting, ihe pollution from aquarulture is becoming more and more serious. The study provided scientific basis for the evaluation of agricultural non-point source pollution in Jiangxi Province.%农业面源污染正成为生态环境的主要问题,但其分散性、广泛性等特点又决定了其治理难度较大.江西目前仍处于农业现代化的起点阶段,农业面源污染日益严重:该研究通过对江西省农业的种植业、畜禽业和水产养殖业的污染源进行调查,利用ArcGIS软件,对江西省农业面源污染进行了空间化和数字化处理.结果表明,江西省农业污染源主要是畜禽养殖业,其次是种植业,水产养殖业污染日趋严重.该研究为江西省农业面源污染评估提供了科学依据.

  8. Status of Agricultural Non-point Source Pollu-tion in China and Com-prehensive Treatment%我国农业面源污染现状及综合治理

    Institute of Scientific and Technical Information of China (English)

    李晓俐

    2015-01-01

    Agricultural non-point source pollution is worsening in China, mainly due to excessive and irrational use of pesticides, excessive use of fertil-izers, plastic sheeting pollution, straw pollution, intensive breeding farms pol-lution,etc. Agricultural non-point source pollution is harmful to water, atmosphere and soil,so the comprehensive treatment measures were put forward.%我国农业面源污染日益严重,主要原因是过量且不合理地使用农药,过量使用化肥,农膜污染、秸秆污染、集约化养殖场污染等。农业面源污染对水体、大气、土壤等造成严重危害,故提出综合治理措施。

  9. 卫星遥感技术在农业非点源污染评价中的应用分析%Analysis of Satellite Remote Sensing Technology in the Evaluation of Agricultural Non- point Source Pollution

    Institute of Scientific and Technical Information of China (English)

    陈强; 胡勇; 巩彩兰

    2011-01-01

    Non - point source pollution is an important source of water pollution, thus constituting one of the decisive factors affecting water environment. The commonly used agricultural non - point source pollution evaluation methods include statistical models and physical models of the computer. No matter what kind of modeling the researchers adopt, a variety of data acquisition types and the evaluation of the accuracy of the verification results make up the main bottleneck. In order to make people aware of the importance of satellite remote sensing technology in agricultural non - point source pollution evaluation, this paper made an application analysis of the access capability and feasibility of the satellite remote sensing technology from the angle of data types required by the study of the non - point source pollution, and also forecast the application potential of the satellite remote sensing technology in the precision verification of the agricultural non - point source pollution evaluation results.%非点源污染物是水污染的重要来源,已成为影响水环境状况的决定性因素之一.目前常用的农业非点源污染评价模型包括统计模型和机理模型两大类,而无论采用哪种建模方法,多类型数据的获取和评价结果的精度验证都是研究的主要瓶颈.为了使人们对卫星遥感技术在农业非点源污染评价中的应用有所了解,从非点源污染研究所需数据种类的角度,对卫星遥感技术的获取能力和可行性进行了应用分析,并对卫星遥感技术在农业非点源污染评价结果的精度验证中的应用潜力进行了展望.

  10. Assessment and Strategies for Watershed Agricultural Non-point Source Pollution Control%集水区农业非点源污染之评估及控制对策

    Institute of Scientific and Technical Information of China (English)

    林昭远; 陈键鑫; 颜正平

    2001-01-01

    Improper agricultural activities accelerate soil loss and water pollution. An assessment system for agricultural non-point source pollution estimation was developed in this study. Digital Elevation Model (DEM), Remote Sensing (RS) and Geographic Information Systems (GIS) couples with non-point source pollution models were applied in the system to discuss the efficiency of non-point pollution control in a watershed. Concepts of sediment delivery ratio (SDR) and placement of riparian buffer strips couples with the analysis of topography and hydrology for the watersheds of interest to classify the sensitive zone could effectively monitor and control watershed agricultural non-point-source pollution.%集水区内不当之农业活动,加速集水区土壤流失及水库水质恶化。本研究利用数值地形模型(Digital Elevation Model, DEM)、配合遥感探测 (Remote Sensing, RS)与地理信息系统(Geographic Information Systems, GIS)等技术,撰写程序建立集水区农业非点源污染评估系统,探讨集水区农业非点源污染控制之成效。利用泥砂递移率与植生缓冲带区位检视集水区内之农业非点源污染源,划定集水区环境敏感区位,针对敏感区回收造林,可有效控制集水区农业非点源污染。

  11. 基于农业面源污染控制的三峡库区种植业结构优化%Planting structure optimization based on agricultural non-point source pollution control in Three Gorges Reservoir Region

    Institute of Scientific and Technical Information of China (English)

    肖新成; 谢德体; 何丙辉; 魏朝富; 倪九派

    2014-01-01

    Agricultural non-point source pollution has gradually become a major pollution source of lake eutrophication and deterioration of water quality in recent years. The Three Gorges Reservoir Region is both an important sensitive eco-economy area and a typical poverty-stricken mountain area. However, its unique geographical location, its irrational agricultural structure and its increasing serious agricultural non-point source pollution problems challenge the environment in this region. Hence, it is a necessity to take controlling agricultural non-point source pollution from the source as one of the most effective approaches. In current research, agricultural non-point source pollution control excessively depends on engineering and technology. Anyhow, the roles of agricultural planting structure adjustment and agricultural cleaner production which can reduce non-point source pollution should not be ignored. Promoting the adjustment of planting structure, reducing the use of chemical fertilizers and pesticides, and adopting agricultural cleaner production technologies not only help improve the land production capacity and agricultural products quality, but also effectively reduce the agricultural non-point source pollution. By these important measures for energy saving and emission reduction can be realized in Three Gorges Reservoir Region. Based on the dual goals of the optimal agricultural economic benefits and non-point source pollution emission reduction, the linear programming model was used to optimize the planting structure and clean agricultural production in the Three Gorges Reservoir Region. The optimization results showed that the optimal paths to maximize the crop net income were as follows: The land area for grain production should cover 119.16×104hm2but cleaner production area of rice-wheat, corn, soybean and tuber crops needs 31.91×104, 18.69×104, 6.79×104and 19.55×104 hm2; the land area for vegetables planting should be 33.25×104hm2 and its cleaner

  12. 兰州农村面源污染现状及防治对策分析%The Analysis of the Rural Non-point Source Pollution Situation and Countermeasures in Lan Zhou City

    Institute of Scientific and Technical Information of China (English)

    桂洪杰; 赵军平; 杜海霞; 张君弟; 刘佳

    2011-01-01

    文中在兰州统计年鉴的基础上,根据面源污染来源,分析了农业、畜牧业、生产和生活活动的危害,初步估算了主要面源污染物的流失量。并根据兰州市农村具体情况提出了相应的保护和控制措施,旨在为解决农村面源污染问题和保护农村生态环境提供借鉴。%In this paper,on the basis of Lan Zhou statistical yearbook,We analyzed the dangers of agriculture,animal husbandry,production activities and life activities according to the sources of rural non-point pollution,and then,we preliminary estimate the loss of the main non-point pollutant.In order to Provide reference for solving the problem of non-point pollution and the rural ecological environment protection,we put forward the corresponding protection and control measures,according to the specific situation of Lan Zhou countryside.

  13. SWAT模型在大洋河流域非点源污染模拟中的运用研究%The application of the SWAT model in a non-point source pollution in the Dayang River basin

    Institute of Scientific and Technical Information of China (English)

    刘晓哲

    2015-01-01

    Based on the SWAT model to Ocean River watershed for the study, quantitative modeling of the ocean River 2000 - -2010 Pollution, the results show that non-point source: SWAT model is appropriate for non-point source pollu-tion ocean river basin model parameters of periodic and verification simulation of total nitrogen and total phosphorus relative er-ror is less than 30%, the simulation uncertainty factor of 0. 5 or more, to meet the non-point source pollution simulation ac-curacy; total nitrogen and total phosphorus peak ocean River mainly 7 - September. Research for Ocean River water environ-mental protection and governance provide a reference value.%基于SWAT模型,以大洋河流域为研究流域,定量模拟了大洋河流域2000 -2010 年非点源污染,研究结果表明:SWAT模型适合于大洋河流域的非点源污染模拟,模型在参数率定期和验证模拟总氮和总磷相对误差均小于30%,模拟确定性系数达到0. 5 以上,满足流域非点源污染模拟精度要求;大洋河流域总氮和总磷峰值主要集中在7 -9 月份. 研究成果对于大洋河流域水环境保护和治理提供参考价值.

  14. A review on Non-point Source Nutrient Pollution of Irrigation Plain Areas%平原灌区农田养分非点源污染研究进展

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    平原灌区作为农业规模化生产的基地,在农业乃至经济社会发展中起到了重要作用,但是随着农业的发展,平原灌区农田非点源污染问题日益严重,研究平原灌区农田非点源污染具有重要意义。文章以平原灌区污染物随水文循环迁移过程为基础对平原灌区养分非点源污染研究进行综述。(1)平原灌区农田非点源污染的主要来源是过量的化肥施用,农药和农膜,秸秆等农作物的降解,牲畜粪便,污水灌溉,灌溉引起的盐渍化以及大气的干湿沉降等;产生及影响因素主要有土壤的理化性质,水分的输入方式和人为管理措施等。(2)降雨径流及灌溉排水条件下污染物在多级渠系中的迁移规律和灌区地表水与地下水的交互耦合作用决定了平原灌区农田非点源污染的输送途径与特征。(3)在监测资料比较缺乏时,采用输出系数法进行负荷估算;在监测资料充足情况下,采用针对灌区特殊的水文特征而改进的经典水文模型对平原灌区农田非点源污染的负荷进行估算。(4)新型肥料、配方施肥、合理的耕作措施及生态沟渠的设置有利于平原灌区农田非点源污染的控制,TMDL (Total Maximum Daily Loads)为平原灌区农田非点源污染控制提供可靠的依据。针对平原灌区非点源污染研究现状,提出了中国开展平原灌区农田非点源污染研究的重点,包括养分污染物在多级沟渠中的迁移,灌区地表与地下水水量水质耦合模型的建立,分级控制单元与TMDL的制定以及多模型结合模拟等,可望在控制农业非点源污染方面起到指导作用。%Plain irrigation areas as the basis of agricultural large-scale production played an important role in agricultural, economic and social development. However, the seriousness of non-point source pollution in plain irrigation areas is increasing with the

  15. Including non-point sfources in a water quality trading permit program.

    Science.gov (United States)

    Collentine, D

    2005-01-01

    There has been overwhelming interest in addressing water quality issues through the use of economic instruments. Much of this attention has focused on the cost efficiencies offered by Transferable Discharge Permit (TDP) systems. Unfortunately, the attempts to start up permit markets which are able to exploit abatement cost differences between sources have not met with the success expected. Two of the reasons for the lack of success that have been taken up in analysis of these programs have been the problem of transaction costs and in the case of non-point sources (NPS), undefined property rights. The composite market design is a proposal for a TDP system which specifically includes agricultural non-point source (NPS) dischargers and addresses both property rights and transaction cost problems. The composite market consists of three interrelated markets each serving a particular function. When the composite market is mature, the total number of permits issued represents the cap on discharges allowed in the catchment. The structure of the composite market allows this system to be phased in over time with existing institutions and limited demands on financing.

  16. Calculation and discussion of non-point source pollution loads in Danjiangkou Reservoir Area%丹江口水库入库非点源污染负荷的计算与讨论

    Institute of Scientific and Technical Information of China (English)

    郑艳霞; 程超; 辛小康

    2015-01-01

    正确估算丹江口水库入库非点源污染负荷对于水源区水环境保护具有重要意义. 根据丹江口库区6条主要入库河流汉江、天河、堵河、丹江、老灌河、淇河控制水文站2013年逐日流量数据,采用数字滤波法对基流进行了分割. 以逐日流量、河川基流量和代表污染物(CODMn和TP)2013年逐月浓度监测值为基础,采用通量法计算了背景污染负荷和点源污染负荷、非点源污染负荷. 结果表明:(1) 6条河流入库流量占总入库流量的95. 9%,非点源污染已成为丹江口水库水质变化的主导因素. (2) 汉江是入库污染负荷的最大来源,其次是堵河. (3) 由于非点源污染伴随降雨汇入河道,水库污染负荷主要集中于丰水期,2013年度丰水期CODMn和TP的比例分别达到了80. 8%和90. 9%.%Correctly estimating the non-point source pollution loads in Danjiangkou Reservoir Area is important to the envi-ronmental protection of the water source area. The Digital Filter method is used to separate the base flows of Hanjiang River, Tianhe River, Duhe River, Danjiang River, Laoguan River and Qihe River in the reservoir basin according to the daily discharge data. Based on the daily flow, the base flow and monthly typical pollutant concentration in 2013, a flux model is set up to calcu-late the background pollution loads, point-source pollution loads and non-point source pollution loads respectively. The calcu-lation results show that in 2013, the inflow discharge of the 6 rivers accounted for 95. 9% of the total inflow, and the total pollu-tion loads of CODMn and TP from the 6 rivers are 58. 2 thousand t and 1. 86 thousand t, while the non-point source pollution loads are 39. 82 thousand t and 1. 544 thousand t, so the non-point source pollution is the decisive factor;Hanjiang River is the largest pollutants contributor to Danjiangkou Reservoir and followed by Duhe River; the non-point source pollution enters into the river channel

  17. 宁夏黄河灌区农业非点源污染损失估算%Economic loss caused by non-point source pollution ——A case study of Ningxia Yellow River Water Irrigation District

    Institute of Scientific and Technical Information of China (English)

    杨引禄; 冯永忠; 杨世琦; 曹艳春; 刘强; 杨改河

    2011-01-01

    Non-point source pollution have become more and more serious and brought enormous danger to water environment in Ningxia Yellow River Water Irrigation District, so it has important significance for ecological security to esti-mate the economic value of losses of pollution load in this area. In this paper, according to the JOHNES export coefficient method, calculation is made of non-point source pollution load in'Ningxia Yellow River Water Irrigation District, and the methoddology of enxironmental economics is used to reckon quantitatively economic loss caused by agricultural non-point source pollution. The results showed that the livestock breeding pollution contributed 41.27% to the pollution load of the area, and 37.25 % to the TN and 4.03 % to the TP pollution load of the area. The planting was the second and the rural domestic waste was the lowest proportion in non-point source pollution load, which was 34.54% and 24.2% to the pollution load of the area. The economic loss caused by the regional non-point source pollution was calculated at about 548.741 million RMB in total, of which about 40.97% was coutributed by livestock breeding pollution, while the planting and the rural areaa domestic waste respectively 35.6% and 23.4% to the all economic loss. This results illuminate that only the control of livestock breeding pollution can reduce economic loss caused by agricultural ono-point source pollution in Ningxia Yellow River Water Irrigation District.%以宁夏黄河灌区为研究区,在充分利用JOHNES输出系数法计算灌区非点源污染负荷的基础上,应用环境经济学中的恢复防护费用法对灌区农业非点源污染产生的负荷转化为经济损失进行估算.结果表明:宁夏黄河灌区禽畜养殖产生的污染负荷最高,占灌区污染负荷的41.27%,其中全氮排放占整个区域污染负荷的37.25%,全磷排放占整个区域污染负荷的4.03%,种植业产生的氮磷污染负荷次之,居民生活

  18. Present Situation and Countermeasures of Agricultural Non-point Source Pollution in Wuhan City%武汉市农业面源污染现状及治理对策

    Institute of Scientific and Technical Information of China (English)

    汪坤乾; 张凯; 苏斌; 夏杏明; 汤少云

    2014-01-01

    从化肥施用、农药使用、农膜使用现状3个方面阐述了武汉市农业面源污染现状和为害性,分析了武汉市农业面源污染产生的原因,提出了建立政府主导的农业面源污染治理机制、应用化肥减量化技术、应用农药减量化和残留控制技术、推广可降解地膜等治理面源污染的对策。%We pointed out the status and harmfulness of agricultural non-point source pollution in Wuhan from the three aspects, including the application of chemical fertilizer, pesticide and agricultural film, and analyzed the factors that caused the problem, in addition, we put forward related countermeasures, including establishing the government leading mechanism to control agricultural non-point source pollution, using chemical fertilizer reduction technology, applying pesticide reduction and residue control technology, and using biodegradable plastic film.

  19. Incorporation of Complex Hydrological and Socio-economic Factors for Non-point Source Pollution Control: A Case Study at the Yincungang Canal, the Lake Tai Basin of China

    Science.gov (United States)

    Yang, X.; Luo, X.; Zheng, Z.

    2012-04-01

    It is increasingly realized that non-point pollution sources contribute significantly to water environment deterioration in China. Compared to developed countries, non-point source pollution in China has the unique characteristics of strong intensity and composition complexity due to its special socioeconomic conditions. First, more than 50% of its 1.3 billion people are rural. Sewage from the majority of the rural households is discharged either without or only with minimal treatment. The large amount of erratic rural sewage discharge is a significant source of water pollution. Second, China is plagued with serious agricultural pollution due to widespread improper application of fertilizers and pesticides. Finally, there lack sufficient disposal and recycling of rural wastes such as livestock manure and crop straws. Pollutant loads from various sources have far exceeded environmental assimilation capacity in many parts of China. The Lake Tai basin is one typical example. Lake Tai is the third largest freshwater lake in China. The basin is located in the highly developed and densely populated Yangtze River Delta. While accounting for 0.4% of its land area and 2.9% of its population, the Lake Tai basin generates more than 14% of China's Gross Domestic Production (GDP), and the basin's GDP per capita is 3.5 times as much as the state average. Lake Tai is vital to the basin's socio-economic development, providing multiple services including water supply for municipal, industrial, and agricultural needs, navigation, flood control, fishery, and tourism. Unfortunately, accompanied with the fast economic development is serious water environment deterioration in the Lake Tai basin. The lake is becoming increasingly eutrophied and has frequently suffered from cyanobacterial blooms in recent decades. Chinese government has made tremendous investment in order to mitigate water pollution conditions in the basin. Nevertheless, the trend of deteriorating water quality has yet to

  20. Assessment of point and non-point pollution associated with the power generation sector in South Africa.

    Science.gov (United States)

    van Zyl, H D; Heath, R G

    2007-01-01

    Access to water and water availability remains a key factor in ensuring the sustainability of development in Southern Africa. The need for guidelines to improve management of this valuable resource, and to regulate pollutant discharge, is therefore of national interest. A new and growing threat to our natural water resources is non-point source (NPS) pollution. The important distinction between point pollution and NPS pollution is that the latter is difficult to identify and the entry point of contamination to resources is diffuse and not limited to a single location. NPS pollution associated with power generation includes, but is not limited to, atmospheric deposition resulting from emissions (air and water), leachate from coal storage piles and runoff from impervious areas which are covered with dust fallout from coal and ash handling operations. Emissions of primary concern are sulfur, nitrogen and mercury.

  1. Spatial Quantification of Non-Point Source Pollution in a Meso-Scale Catchment for an Assessment of Buffer Zones Efficiency

    Directory of Open Access Journals (Sweden)

    Mikołaj Piniewski

    2015-04-01

    Full Text Available The objective of this paper was to spatially quantify diffuse pollution sources and estimate the potential efficiency of applying riparian buffer zones as a conservation practice for mitigating chemical pollutant losses. This study was conducted using a semi-distributed Soil and Water Assessment Tool (SWAT model that underwent extensive calibration and validation in the Sulejów Reservoir catchment (SRC, which occupies 4900 km2 in central Poland. The model was calibrated and validated against daily discharges (10 gauges, NO3-N and TP loads (7 gauges. Overall, the model generally performed well during the calibration period but not during the validation period for simulating discharge and loading of NO3-N and TP. Diffuse agricultural sources appeared to be the main contributors to the elevated NO3-N and TP loads in the streams. The existing, default representation of buffer zones in SWAT uses a VFS sub-model that only affects the contaminants present in surface runoff. The results of an extensive monitoring program carried out in 2011–2013 in the SRC suggest that buffer zones are highly efficient for reducing NO3-N and TP concentrations in shallow groundwater. On average, reductions of 56% and 76% were observed, respectively. An improved simulation of buffer zones in SWAT was achieved through empirical upscaling of the measurement results. The mean values of the sub-basin level reductions are 0.16 kg NO3/ha (5.9% and 0.03 kg TP/ha (19.4%. The buffer zones simulated using this approach contributed 24% for NO3-N and 54% for TP to the total achieved mean reduction at the sub-basin level. This result suggests that additional measures are needed to achieve acceptable water quality status in all water bodies of the SRC, despite the fact that the buffer zones have a high potential for reducing contaminant emissions.

  2. A novel modelling framework to prioritize estimation of non-point source pollution parameters for quantifying pollutant origin and discharge in urban catchments.

    Science.gov (United States)

    Fraga, I; Charters, F J; O'Sullivan, A D; Cochrane, T A

    2016-02-01

    Stormwater runoff in urban catchments contains heavy metals (zinc, copper, lead) and suspended solids (TSS) which can substantially degrade urban waterways. To identify these pollutant sources and quantify their loads the MEDUSA (Modelled Estimates of Discharges for Urban Stormwater Assessments) modelling framework was developed. The model quantifies pollutant build-up and wash-off from individual impervious roof, road and car park surfaces for individual rain events, incorporating differences in pollutant dynamics between surface types and rainfall characteristics. This requires delineating all impervious surfaces and their material types, the drainage network, rainfall characteristics and coefficients for the pollutant dynamics equations. An example application of the model to a small urban catchment demonstrates how the model can be used to identify the magnitude of pollutant loads, their spatial origin and the response of the catchment to changes in specific rainfall characteristics. A sensitivity analysis then identifies the key parameters influencing each pollutant load within the stormwater given the catchment characteristics, which allows development of a targeted calibration process that will enhance the certainty of the model outputs, while minimizing the data collection required for effective calibration. A detailed explanation of the modelling framework and pre-calibration sensitivity analysis is presented.

  3. Evaluation of Land Use, Land Management and Soil Conservation Strategies to Reduce Non-Point Source Pollution Loads in the Three Gorges Region, China.

    Science.gov (United States)

    Strehmel, Alexander; Schmalz, Britta; Fohrer, Nicola

    2016-11-01

    The construction of the Three Gorges Dam in China and the subsequent impoundment of the Yangtze River have induced a major land use change in the Three Gorges Reservoir Region, which fosters increased inputs of sediment and nutrients from diffuse sources into the water bodies. Several government programs have been implemented to mitigate high sediment and nutrient loads to the reservoir. However, institutional weaknesses and a focus on economic development have so far widely counteracted the effectiveness of these programs. In this study, the eco-hydrological model soil and water assessment tool is used to assess the effects of changes in fertilizer amounts and the conditions of bench terraces in the Xiangxi catchment in the Three Gorges Reservoir Region on diffuse matter releases. With this, the study aims at identifying efficient management measures, which should have priority. The results show that a reduction of fertilizer amounts cannot reduce phosphorus loads considerably without inhibiting crop productivity. The condition of terraces in the catchment has a strong impact on soil erosion and phosphorus releases from agricultural areas. Hence, if economically feasible, programmes focusing on the construction and maintenance of terraces in the region should be implemented. Additionally, intercropping on corn fields as well as more efficient fertilization schemes for agricultural land were identified as potential instruments to reduce diffuse matter loads further. While the study was carried out in the Three Gorges Region, its findings may also beneficial for the reduction of water pollution in other mountainous areas with strong agricultural use.

  4. Evaluation of Land Use, Land Management and Soil Conservation Strategies to Reduce Non-Point Source Pollution Loads in the Three Gorges Region, China

    Science.gov (United States)

    Strehmel, Alexander; Schmalz, Britta; Fohrer, Nicola

    2016-11-01

    The construction of the Three Gorges Dam in China and the subsequent impoundment of the Yangtze River have induced a major land use change in the Three Gorges Reservoir Region, which fosters increased inputs of sediment and nutrients from diffuse sources into the water bodies. Several government programs have been implemented to mitigate high sediment and nutrient loads to the reservoir. However, institutional weaknesses and a focus on economic development have so far widely counteracted the effectiveness of these programs. In this study, the eco-hydrological model soil and water assessment tool is used to assess the effects of changes in fertilizer amounts and the conditions of bench terraces in the Xiangxi catchment in the Three Gorges Reservoir Region on diffuse matter releases. With this, the study aims at identifying efficient management measures, which should have priority. The results show that a reduction of fertilizer amounts cannot reduce phosphorus loads considerably without inhibiting crop productivity. The condition of terraces in the catchment has a strong impact on soil erosion and phosphorus releases from agricultural areas. Hence, if economically feasible, programmes focusing on the construction and maintenance of terraces in the region should be implemented. Additionally, intercropping on corn fields as well as more efficient fertilization schemes for agricultural land were identified as potential instruments to reduce diffuse matter loads further. While the study was carried out in the Three Gorges Region, its findings may also beneficial for the reduction of water pollution in other mountainous areas with strong agricultural use.

  5. 西充河流域(西充县境内)面源污染现状与防治措施%Non-point Source Pollution and Control Measures of Xichong River Basin (Xi-chong Territory)

    Institute of Scientific and Technical Information of China (English)

    王小霞; 黎小东; 张洪波; 刘星; 吴碧琼

    2015-01-01

    Taking for example the 23 towns ,in Xichong River Basin (Xi-chong territory) ,the output coefficient method is used to e‐valuate the rural non-point source pollution comprehensively from the perspective of the various sources and different towns and put forward some corresponding control measures .The results show that the main pollution in the research area is the rural non-point source pollution :①83 .3% of the pollution load comes from chemical fertilizer pollution ,rural residents living sewage and backyard poultry ;②The pollution in Hong-xi River is more serious than Longtan River;③The most polluted village is Jin-cheng Town .Ac‐cording to the results of this evaluation ,this paper puts forward the corresponding comprehensive control of non-point source pollu‐tion in engineering and non-engineering measures and suggestions .%以西充河流域(西充县境内)的23个乡镇为例,采用输出系数法,从各个污染源和不同乡镇的角度出发,对西充县境内的农村面源污染进行了综合评价,并提出相应的防治措施。结果表明:研究区内的主要污染为农村面源污染:①83.3%的污染负荷来源于为化肥污染、农村居民生活污水和散养畜禽;②虹溪河支流与龙滩河相比,污染较严重;③污染最严重的乡镇为晋城镇。根据评估结果,本文提出了相应的面源污染综合治理的工程及非工程措施和建议。

  6. Non-pointSource Pollution Control Policy under the Perspective of Experimental Economics:A Review%实验经济学视角下的非点源污染控制政策研究:一个文献综述

    Institute of Scientific and Technical Information of China (English)

    Wu Dan; Zhang weiwen

    2015-01-01

    Non-point source pollution has become an important source of water pollution for many countries including China. Used for the design and evaluation of non-point pollution control policy recently,experimental economics has made remarkable achievements in this field. In contrast,related research has not been published in China so far. This paper tries to anatomize and conclude the experimental economics on non-point source control policy by summarizing it as two categories,that is,individual -performance -based and collective -performance -based, from which some enlightenment are obtained and some preliminary research ideas are put forward.

  7. Source Apportionment of Non-point Source Nitrogen Pollution in Ashi River Basin Usingδ15N Technique%阿什河流域非点源氮污染的δ15N源解析研究

    Institute of Scientific and Technical Information of China (English)

    胡钰; 王业耀; 滕彦国; 香宝; 马广文; 方广玲

    2015-01-01

    crop farming on water nitro-gen pollution were dependent on river water seasonal periods and farming cycles. During the normal water period, non-point nitrogen pollu-tion by crop farming occurred mainly in the midstream and downstream sections, with δ15N value ranging from 0.46%to 0.77%. The pollu-tion sources were primarily artificial chemical fertilizers and farmland water recession. During the high water period, the pollution from non-point nitrogen extended to the upstream area, withδ15N range of 0.19%~0.4%. The main pollution source was artificial chemical fertilizers in the upstream area, while it was soil organic nitrogen via soil erosion caused by rainfall and irrigation in the midstream and downstream. Dur-ing the low water period, however, artificial chemical fertilizers were still the main contributor of non-point nitrogen pollution, with δ15N val-ues of 0.11%to 0.39%. This was resulted from the feedback of the nitrite-nitrogen from artificial chemical fertilizers that were applied and infiltrated in the ground during the monsoon.

  8. 重庆市四面山4种森林群落面源污染控制功能分析%The non-point source pollution control function of four forest communities in Simian Mountains, Chongqing

    Institute of Scientific and Technical Information of China (English)

    荣文卓; 张洪江; 杜仕才; 程金花; 王伟; 李根平; 古德洪

    2009-01-01

    Selecting canopy interception rate, capillary porosity,non-capillary porosity and soil anti-erosion coefficient as analysis indices, the function of non-point source pollution control of four forest communities in Simian Mountains of Chongqing were ana-lyzed by Grey Relational Grade Analiysis. The results show that the grey correlation degree of natural Phyllostachys pubescens for-est is 0.887 7, hgher than other three communities, which means that the function of non-point source pollution control of Phyl-lostachys pubescens forest is the best. In the planatations types, the grey correlation degree of broadleaf forest is the highest, 0.779 4, followed by mixed broadleaf-conifer forest (0.699 2). The grey correlation degree of coniferous forest is the lowest, 0.636 1. Which means it has the lowest function for non-point source pollution control.%以重庆市四面山4种森林群落为研究对象,选取林冠截留率、林地土壤毛管孔隙度、林地土壤非毛管孔隙度和林地0~20 cm土壤抗冲刷系数为分析指标,运用灰色关联度法对4种森林群落的面源污染控制功能进行研究.结果表明:在4种森林群落中作为天然林的楠竹林灰关联度值最大为0.887 7,面源污染控制功能最好,其次是人工阔叶林(0.7794)和人工混交林(0.6992),人工针叶林(0.636 1)面源污染防控能力相对较弱.在人工林方面,阔叶林灰关联度值最大为0.7794,面源污染防控能力最好,其次是针阔混交林(0.699 2),针叶林灰关联度值最低为0.636 1,其面源污染防控能力较弱.

  9. Agriculture Non-Point Source Pollution Control

    OpenAIRE

    2003-01-01

    The Chesapeake Bay is the largest and historically most productive estuary in the United States. It is approximately 200 miles long and 35 mile wide at it broadest point. The Bay's watershed includes parts of six states (Delaware, Maryland, New York, Pennsylvania, Virginia, West Virginia, and the entire District of Columbia. This area encompasses 64,000 square-miles, 150 major rivers and s...

  10. 国内外遥感技术在非点源污染模拟中的应用%Application of Remote Sensing Technology in Non-point Source Pollution Monitoring

    Institute of Scientific and Technical Information of China (English)

    王天培

    2011-01-01

    综述了遥感技术在土壤类型和性质、植被类型和性质、水文气象以及土地利用等直接影响非点源污染产生的因素中的应用,以期为模型模拟与遥感技术联合研究非点源污染提供借鉴.%The application of remote sensing technology on the main factors, including soil types and properties, characteristics of vegetation,hydrological process, meteorological information, land use and so on were discussed in this paper so as to provide references for using modelsimulation and remote sensing technology to study the non-point source pollution.

  11. 英国农业面源污染防控对我国的启示%Prevention and control of agricultural non-point source pollutions in UK and suggestions to China

    Institute of Scientific and Technical Information of China (English)

    刘坤; 任天志; 吴文良; 孟凡乔; Jessica Bellarby; Laurence Smith

    2016-01-01

    Currently, the world is facing challenges of maintaining food production growth while improving agricultural ecological environ-mental quality. The prevention and control of agricultural non-point source pollution, a key component of these challenges, is a systematic program which integrates many factors such as technology and its extension, relevant regulation and policies. In the project of UK-China Sustainable Agriculture Innovation Network, we undertook a comprehensive analysis of the prevention and control technology, technology extension systems and related policy measures of agricultural non-point source pollution in UK. We then proposed the promotion of preven-tion and control of agricultural non-point source pollution in China. Considering the current agricultural resources and environment counter-measures of“one control”(control over the total quantity of the agricultural water and agricultural water environment pollution),“two re-duction”(reduction in quantities of fertilizers and pesticides), and “three basic countermeasures”(resource utilization, recycling, and disharm treatment of livestock and poultry wastes, agricultural plastic films and crop straws), we suggested that in the near future source control and process prevention should be technical priorities. Optimzation and reduction of chemical fertilizer, combined use of chemical and organic fertilizers, land utilization management, agricultural sector adjustment and integration of crop production with animal husbandry should be the key technologies. The role of non-governmental technical service should be promoted. Government subsidies, ecological com-pensations and other forms of economic means could be used to encourage famers actively participate in the prevention and control of nitro-gen and phosphorus losses and pollution in soil and water systems. Related technical documents and regulations should be more specific, clear, and practical. Collection, transmission and analysis of

  12. Investigation on Present Xingtai Agricultural Non-point Source Pollution and Preventive Countermeasures%邢台市农业面源污染现状及防治对策

    Institute of Scientific and Technical Information of China (English)

    陈计兵

    2014-01-01

    Xingtai has a large population, with a serious contradiction between demand for food and land supply. The development of agriculture has brought about some pollution, and agricultural non-point source pollution prevention and control is the basic guarantee to realize agricultural sustainable development. Xingtai agricultural non-point source pollution is serious. The pollutants mainly include chemical fertilizer, chemical pesticide, agricultural plastic films, livestock and poultry breeding wastes and life wastes. The pollution has caused the decrease of the farmland quality, the degradation of the quality of agricultural products and water, the damage of the ecological balance. The countermeasures include technical measures, management measures, publicity and education measures, etc.%邢台市人口数量大、粮食需求、土地供给矛盾突出,在农业发展中,产生了一些不可忽视的污染现象,而农业面源污染的防治是实现农业可持续发展的基本保障。邢台市农业面源污染比较严重,污染源主要包括化学肥料污染、化学农药污染、农膜污染、畜禽养殖业污染和生活废弃物污染;污染带来耕地质量下降、农产品和水质质量变差、破坏生态平衡等危害;防治对策包括技术措施、管理措施及宣传教育措施等。

  13. NPS TINYSCOPE Program Management

    Science.gov (United States)

    2010-09-01

    orbit and the rotation of the Earth and Moon . The planets will also perturb the orbits with Jupiter and Venus acting as the primary sources...4. TITLE AND SUBTITLE NPS TINYSCOPE Program Management 6. AUTHOR( S ) Christopher Gordon Turner 5. FUNDING NUMBERS 7. PERFORMING ORGANIZATION...NAME( S ) AND ADDRESS(ES) Naval Postgraduate School Monterey, CA 93943-5000 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING /MONITORING

  14. 模拟排水沟渠非点源溶质氮迁移实验研究%Transformation of Non-point Source Soluble Nitrogen in Simulated Drainage Ditch

    Institute of Scientific and Technical Information of China (English)

    李强坤; 宋常吉; 胡亚伟; 彭聪; 马强; 姜正曦; 琚艺萌

    2016-01-01

    The drainage ditch has a compound ecosystem structure consisting of water, sediment and plants. Migration and transformation of the non-point source solute is important to study interception, control and management of agricultural non-point source pollution in the drainage ditch. Based on the experiment on static simulation of drainage ditches, the article used typical non-point source soluble nitrogen as an example to analyze the changing process of nitrogen content in water, sediment and reeds, and to study the effects of the sediment adsorption and desorption, reeds growth and death in different periods on nitrogen concentration in water. The article discussed nitrogen migration in water-sediment- reeds compound ecosystem and its influence on nitrogen concentration in water. The results showed that both adsorption and desorption in sediment and absorption and assimilation of reeds growth had effect on nitrogen concentration in water. The effect before October was reducing the nitrogen concentration in water, which was the process of nitrogen purification in water. After October, the nitrogen concentration in water increased and made it easy to form secondary nitrogen pollution. Meanwhile, the migration in the water-sediment-seeds ecosystem in simulated drainage ditch had close ties, any migration and transformation of nitrogen in a single medium or between different mediums would cause adjustment of nitrogen concentration in water.%农田排水沟渠是由水-底泥-植物组成的复合生态结构,其间非点源溶质的迁移转化对研究沟渠拦截、控制和管理农业非点源污染具有重要意义。本研究以模拟排水沟渠静态实验为基础,以典型非点源溶质氮素为例,分析了实验期内水体、底泥及芦苇不同介质内氮含量变化过程,分析了底泥吸附与解析、芦苇生长与衰败等年内不同时期对水体中氮素浓度的影响,探讨了水-底泥-芦苇复合生态体内氮迁移及对水体中氮

  15. Research Progress on Guangdong Agricultural Non-point Source Pollution Control-Conservation Agriculture Project%广东农业面源污染治理保护性耕作项目研究进展

    Institute of Scientific and Technical Information of China (English)

    任小平; 区颖刚; 杨丹彤; 唐湘如; 胡建广

    2015-01-01

    广东省属于农业大省,近年来随着人口增加和农村经济快速发展,农业面源污染形势十分严峻.广东省承担的世界银行农业面源污染治理保护性耕作项目,通过在省内的项目示范区域进行水稻和甜玉米的示范试验,探索保护性耕作减少农业面源污染源的作用和方法.项目组设计了免耕和少耕插秧、免耕和少耕直播4种主要模式,投入了50多台套农业机械,经过4个试验点1年的试验,初步结果表明,南方高温潮湿地区水稻和甜玉米生产采用保护性耕作技术是有效果的,所选的农机具大多数是适用的,部分北方旱地机械还需要改进.讨论了广东保护性耕作存在的问题和改进的意见.%Guangdong province is an important agricultural province. The agricultural non-point source pollution situation is very grim in recent years as the population increases and the rural economy grows rapidly. Explo-ration, supported by the world bank agriculture non-point source pollution control-conservation tillage project, has been undertaken in Guangdong province to discover the roles and methods for reducing agricultural non-point source pollution by conservation tillage. The project is conducted in four paddy rice and sweet corn demonstration and experimental sites in the province. Four main models, the no-till and less-till transplanting, no-till and less-till direct seeding, have been designed by the project team. More than 50 sets of agricultural machines have been put into the experiments. After one year's experiments conducted in four sites, prelimi-nary results show that the conservation tillage technology is effective in the paddy rice and sweet corn produc-tion in southern area with high temperature and wet conditions. Most of the selected farm machines are appli-cable. Some of the northern dryland machines need to be improved. The paper discussed the problems and im-provement opinions existing in the Guangdong conservation

  16. Review of green roof in controlling unban non-point source pollution%绿色屋顶技术控制城市面源污染应用研究进展

    Institute of Scientific and Technical Information of China (English)

    王书敏; 于慧; 张彬; 邵磊

    2011-01-01

    It has been widely adopted abroad that green roof is an effective mode in controlling Urban Non-point Source Pollution(UNSP),which still belongs to new research fields in China.Based on studies in recent years home and abroad,highlight is given about green roof in controlling unban non-point source pollution from three aspects(i.e.,the reduction and retention of runoff volume,water quality from green roof and the installation method of green roof).Results show that further study is needed for a long time in order to establish the function between stormwater runoff reduction volume and influencing factors;the physical and chemical properties of plants growth media is the key problem affecting runoff water quality.It should follow a wide rang of purposes to install green roofs at home,and the design guidance for green roofs should also be made according to domestic environmental background.The plants growth substrate and the choice of plants are primary problems to be ascertained.%应用绿色屋顶技术控制城市面源污染在欧美国家已得到广泛认同和应用,但在国内尚处于起步阶段.根据国内外最近几年的研究情况,从绿色屋顶消减暴雨径流、绿色屋顶径流水质、绿色屋顶构建方法等方面详细介绍了绿色屋顶技术在控制城市面源污染中的应用研究情况.研究结果表明,建立绿色屋顶暴雨径流消减量与影响因子的映射关系仍需要大量的、长期的研究;植物生长介质的理化性质是影响绿色屋顶径流水质的关键.在国内构建绿色屋顶,应遵循构建目的多元化的原则,逐步建立起适合国内国情的新的绿色屋顶构建导则,其中,植物生长基质搭配方式和植物选取是需要重点明确的问题.

  17. Application of SWMM in the Simulation of Non-Point Source Pollution Load in Urban Residential Area%SWMM模型应用于城市住宅区非点源污染负荷模拟计算

    Institute of Scientific and Technical Information of China (English)

    马晓宇; 朱元励; 梅琨; 张艳军; 张明华

    2012-01-01

    This study was focused on the non-point source pollution loading in a typical urban residential area in Wenzhou City, Zhejiang Province. Based on the SWMM principle, parameters from the literature and field experiments were integrated, and a non-point source pollution calibration model was constructed. Four different rainfall scenarios were designed to analyze the pollution loads and the accumulation process of total suspended solids (TSS) , chemical oxygen demand (CODCr) , total nitrogen (TN) and total phosphorus ( TP). The results showed that the simulated data matched the field-monitored data satisfactorily. The relative errors between the simulated and the true values of all the four parameters were less than 10%. Under' the four rainfall conditions: 1) the highest concentrations of the pollutants were observed within 30-40 minutes; the heavier the rain, the earlier their highest concentrations appeared; 2) higher-intensity rainfall caused more serious pollution to the receiving water bodies than the low-intensity rainfall did.%以温州市典型住宅区非点源污染为对象,基于SWMM(storm water management model)模型的模拟机理,借鉴国内外相关研究的模型参数,结合降雨径流实测数据率定模型参数,将模型“本地化”,构建了基于SWMM模型的研究区非点源污染负荷计算模型,并设计了4种不同降雨情景,分析在不同降雨条件下研究区非点源污染固体悬浮物(TSS)、CODCr、TN和TP的污染负荷量及其累积变化过程.结果表明,构建的SWMM模型的模拟值可以较好地与实测值相吻合,4种污染物模拟的相对误差均小于10%.在设计的4种降雨情景下:①污染物浓度峰值出现在降雨30~40 min内,降雨强度越大,出现浓度峰值的时间越早;②高强度降雨较低强度降雨可对受纳水体造成更大的污染.

  18. 我国农业面源污染现状及其对策研究%Review of the current situation and control countermeasures in agricultural non-point source pollution control in China

    Institute of Scientific and Technical Information of China (English)

    李自林

    2013-01-01

    随着我国经济的快速发展,农业面源污染已成为我国环境污染的主要原因之一。农业面源污染制约农业可持续发展,已成为亟待解决的环境问题。目前,造成农业面源污染的原因主要是化肥农药的过量施用和流失,畜禽和水产养殖业污染,水土流失,作物秸秆大量废弃以及农膜污染。农业面源污染具有分散性、广泛性、随机性等特征,对土壤、水体、大气造成负面影响。本文针对农业面源污染问题,从生物、物理、化学以及农业管理等角度提出了相应的防治对策及措施,以期有效治理农业面源污染,保护环境,促进农业可持续发展。%Agricultural non-point source pollution is the main factor to the environmental pollution in rural China now with the development of economy in China .It has gained the attention of the government and scientist in the country , and also is the main factor to restrain the agricultural sustainable development .At present ,the reasons are list as follow :chemical fertilizer and pesticide and excessive application ;Livestock and aquatic products pollution ;soil erosion ;a ma-jority of strews abandoned ;agricultural plastic film pollution .It has the characteristics of dispensability ,universality and randomness ,which have negative effects on soil ,water and air .In terms of biological chemistry and physics and agricul-tural management ,this paper proposes countermeasures to control agricultural non -point source pollution ,protect the environment ,and promote the sustainable development of agriculture .

  19. 潮河流域非点源污染关键区识别及其管理措施研究%IDENTIFICATION OF NON-POINT SOURCE POLLUTION AND ASSESSMENT OF MANAGEMENT MEASURES IN THE CHAO RIVER BASIN

    Institute of Scientific and Technical Information of China (English)

    唐芳芳; 徐宗学; 徐华山

    2012-01-01

    选取SWAT模型,对潮河流域径流、泥沙及非点源污染过程进行模拟.在对模型进行率定和验证的基础上,参照土壤侵蚀模数与国家地表水环境质量标准对土壤侵蚀及总氮污染关键区进行识别.最后分别在土壤侵蚀和总氮污染关键区设置污染控制情景,对控制措施成效进行模拟和评价.结果表明,SWAT模型可以较好地模拟潮河流域水文过程和污染物迁移转化过程;流域20.9%的区域为轻度土壤侵蚀区,39.8%的区域为总氮风险区;梯田、等高耕作、退耕还林还草和减少化肥施用量等措施都不同程度地对非点源污染负荷起到一定的削减效果.%Chao River basin is one of the most important surface water sources for drinking water in Beijing.In recent years,the Chao River basin is facing water scarcity and water quality problem due to the impact of human activities,development of local economy and climate change.The Chao River basin was selected as the study area in this investigation.SWAT model was applied to simulate hydrological cycle and process of nutrient movements and transformation in the Chao River basin.Then critical source areas of soil erosion were identified according to soil erosion modulus,and critical source areas of TN concentration were identified based on the National Environmental Quality Standards for Surface Water.Finally,several scenarios to control non-point source pollution on critical source areas of soil erosion and TN concentration were proposed.Data showed that the performance of calibration and validation for runoff,sediments and nutrients were satisfactory.Mild soil erosion area was 1260.657 km2,accounting for 20.9% of the total basin area,while critical source area of TN was 2405.1 km2,accounting for 39.8% of the total area.Data also showed that the measures including terraces,contour farming,turning farm into forest and grasslands and fertilizer reduction played different reduction role on controlling

  20. 流域非点源污染景观源汇格局遥感解析%Remote sensing parsing on non-point pollution landscape source and assembly pattern in river basin

    Institute of Scientific and Technical Information of China (English)

    张新; 程熙; 李万庆; 罗雷

    2014-01-01

    流域非点源污染景观源汇格局解析是流域水质污染物产生、输运机理认知与有效防治措施制定的重要科学理论基础。该文以充分挖掘流域遥感数据多时空尺度的特征与优势为目标,制定了流域非点源污染景观源汇类型界定方案,提出了像元级、亚像元级以及时序更新的流域非点源污染景观源汇格局遥感解析方法。在此基础上,以福建省九龙江流域为例,对不透水面这一典型流域非点源污染“源”景观2010年空间格局进行了信息提取,并对2000、2005年对应信息进行了时序更新与空间特征分析,结果显示2000-2010年间九龙江流域内不透水面覆盖面积增长了33.38%。该研究可为中国流域非点源污染研究、生态管理与建设提供参考。%The non-point pollution landscape source and assembly pattern of a river basin and its spatio-temporal process analysis are the scientific theoretical bases for studying the pollution, production, transport, prevention, and policy of river water. In this paper, to mine the spatio-temporal character and advantages of remote sensing data, the definition schedule of the landscape of the river basin non-point pollution source and assembly was introduced. The first types of landscape in the river basin included the impervious surface area (ISA) and the pervious surface area. The second types of landscapes in the river basin consisted of 14 different types, including woodland, orchard, road, rural area, town, and paddy field, etc. The river basin non-point pollution landscape source and assembly pattern remote sensing parsing method was subsequently presented at the pixel, sub-pixel, and time-renewing levels. (1) A “globe-local” coupling information extraction model for ISA at the pixel level was established. Through the mining and integration of the spatial information in a local image area, the spectral instability of the whole scale was optimized. The

  1. 苏州市农业面源污染现状与控制研究%Current situation and control recommendations of agricultural non-point source pollution in Suzhou City

    Institute of Scientific and Technical Information of China (English)

    张贵龙; 秦伟; 管永祥; 邱丹; 杨殿林; 赵建宁

    2011-01-01

    Based on the results of national survey of pollution sources, the production and emission characteristics of pollutants from agricultural activities and the major control measures of agricultural non-point source pollution in Suzhou along the Yangtze River, Taihu Lake and Yangchenghu Lake were analyzed and demonstrated. The results showed that among the non-point source pollutants, the totalamount of nitrogen and phosphorus emitted were 11 878.9 and 1 343.8 t respectively, pesticides loss was 28.0 t, plastic membrane residual was 203.9 t, the volume of straw burned was 214 000 t, total amount of COD emitted by livestock breeding, aquaculture and rural community was 92 360.3 t; The emission amount of nitrogen and phosphorus along the Yangtze River was the largest followed by Taihu Lake, along the Yangcbenghu Lake the lowest. According to the analysis of the total amount of N and P emitted, the ranking of pollutant resource was as below: rural community > livestock breeding >aquaculture > planting; The pollution intensity caused by the four sources were different. The three watershed, the pollution intensity of farming, animal husbandry and rural communities was greater in the Yangtze River, but the pollution intensity of aquaculture was greater in Taihu Lake Therefore,in guiding concept for the prevention and control of no point source pollution, the government should pay close attention to the Yangtze River, give more weight to domestic pollution sources. In view of the above -mentioned facts,to control agricultural non-point source pollution. Some proposals were offered as follows, concentrating superior resources to dispose rural sewage, optimizing the agricultural industry structure, strengthening the ecological construction, comprehensively treating livestock pollution, promoting the utilization of crop straw resources,and reasonably planning aquaculture etc.%利用提供的区城产排污系数,对苏州市沿长江、太湖和阳澄湖流域农业面

  2. SWAT模型在洱海流域面源污染评价中的应用%Application of SWAT Model in Agricultural Non-Point Source Pollution Investigation in Lake Erhai Watershed

    Institute of Scientific and Technical Information of China (English)

    翟玥; 尚晓; 沈剑; 王欣泽

    2012-01-01

    重点污染区域和污染因子的识别是面源污染控制的基础.通过将物理过程模拟及排污系数法计算进行整合,建立了SWAT模型,以描述农业生产活动与污染入湖量之间的关联关系,并以云南洱海流域总氮污染为例,使用验证后的SWAT模型模拟计算不同空间单元和不同农业生产活动对入湖TN的污染贡献系数,定量分析流域内各区域的农业面源污染源结构,识别洱海流域重点农业污染源和农业污染村镇.结果表明,奶牛养殖、生猪养殖和大蒜种植是目前洱海流域内入湖TN污染的最重要农业污染源,占流域总污染负荷的66.12%.对入湖TN污染贡献最大的6个村镇为江尾、右所、三营、玉湖、凤仪和喜洲,占流域总污染负荷的63.41%.%The identification of key polluted areas and factors is the basis of non-point source pollution control. Based on physical processes simulation and the discharge coefficient method, the SWAT model was established to describe the relationship between agricultural production activities and pollution load into a lake. Lake Erhai watershed in Yunnan province was selected as the research area, and TN variation in the lake was calculated. The contribution coefficients of TN nonpoint source pollution to the lake by different spatial areas and different agricultural production activities were calculated with the verified SWAT model, and the pollution source composition in each spatial unit was calculated quantitatively. The key pollution sources and Bpatial units ( administrative village in this study) were identified. The results showed that dairy cattle breeding, pig farming and garlic planting were the major agricultural pollution sources, contributing 66.12% of TN load to the lake. Six villages - Jiangwei, Yousuo, Sanying, Yuhu, Fengyi and Xizhou - contributed most to TN pollution in the lake, accounting for 63.41% of the watershed total pollution load.

  3. Yield calculation of agricultural non-point source pollutants in Huntai River Basin based on SWAT model%基于SWAT模型的浑太河流域农业面源污染物产生量估算

    Institute of Scientific and Technical Information of China (English)

    付意成; 臧文斌; 董飞; 付敏; 张剑

    2016-01-01

    The establishment of non-point source pollutants output load model under the mode of rainfall-runoff and land use, the analog calculation of agricultural non-point source pollutants in the process of migration and transformation, and the systematic analysis of non-point source pollutants discharge quantity, distribution and composition characteristics are based on actual monitoring data, calibration and validation model, in consideration of underlying surface, hydrology and meteorology, and physical features of Huntai River basin. The areas 1 km away from each side of master stream Huntai River, Taizihe River and Daliaohe River and 5 km away from reservoir were defined as buffer zone, where the mode of land use was transformed so as to restore the natural ecosystem. The process of pollutant migration and conversion was simulated based on the calibration of key hydrological parameters, and the causes as well as the migratory features of non-point source pollution were investigated. The primary area of water environment pollution was mainly distributed along both sides of the water channel of the mainstreams of Huntai River. The point-source pollutant was mainly related to the distribution of industry and the amount of discharged wastewater. The risk of non-point pollution was mainly related to the pattern of agricultural plantation and farmland utilization. The secondary area of water environment pollution was mainly distributed along both sides of the water channel of tributaries. Therefore, the situation of pollutant production corresponding to the intra-regional regulation of industrial structure, land utilization pattern surrounding the water channel should be highlighted. The non-point pollution in Huntai watershed was dominated by farmland pollution, and the main indices of pollutants were total nitrogen (N) and total phosphorus (P). The contribution rate of pollutants was farmland runoff > livestock and poultry breeding > urban runoff > water and soil erosion

  4. NPS AUV Integrated Simulator

    Science.gov (United States)

    1992-03-01

    147 3. GRAPHIC OBJECT MODEI’QUSING OBJECTFILE FORMAT (cIF)...................... ........... 149. APPENDIX -C. NPS, AUV SONAR CLASSIFICATION SYSTEM...Monterey Bay .. 15 Figure 2.8 Graphics simulation for NPS AUV sonar visualization .......... 15’ Figure 3.1 Integrated simulator network physical...3.11 Integrated simulation display of AUV minefield searh ........ 39 Figure4.1 NPS AUV sonar beam profiles in the NPS pool .......... 43 Figure 42 NPS

  5. 生态景观型灌排系统面源污染防治试验及生态响应%Non-point source pollution control experiment and ecological response of ecological landscape type irrigation and drainage system

    Institute of Scientific and Technical Information of China (English)

    张雅杰; 邵庆军; 李海彩; 叶梁倩; 姚星; 胡将军

    2015-01-01

    The current irrigation and drainage system in land renovation was mainly designed and constructed to heighten efficiency of irrigation and drainage, structure safety and convenient management, which, however, somewhat neglected ecological landscape design, exerting an inappropriate influence on the biological habitat and agricultural non-point source pollution. Therefore, the aim of this paper is to design an ecological landscape irrigation and drainage system and realize more comprehensive benefits. First of all, originated from the traditional agricultural drainage and irrigation system, irrigation and drainage with an ecological ring-ditch was designed by using the principle of mutually-beneficial symbiosis of paddy, red duckweed, fish and vegetable planting plates, which realized the multifunctional stereo development of rice paddy pisciculture, vegetable planting and so on. Meanwhile, the roundabout biochemical pond was designed at the ecological ring-ditch drainage sluice, which was used as the treatment device of agricultural non-point source pollution. Then, ecological landscape lateral canal and lateral ditch was designed, which was made up of vegetation type diaphragm blocks, hornworts, vegetable planting plates, fish, Jumping type antiskid ecological plate and plant ecological community. What is more, by using the land reclamation project in shanghai as the experimental area, the rice growth cycle (6-10 months) as study period, the COD, NH4+-N, TN and TP as test indicators, and three monitoring sections as monitoring points, experimental of non-point source pollution prevention effects were made comparisons between system 1, system 2 and system3. The system 1 was constituted by the irrigation and drainage system using ecological ring-ditch, roundabout biochemical pond and lateral ditch wetlands. The system 2 was constituted by the irrigation and drainage system using underground pipe and current lateral ditch. And the system 3 was constituted by the

  6. 宁夏农村面源污染现状调查分析结果初报%Preliminary Report on Investigation and Analysis Result of Current Situation of Rural Non-point Source Pol ution in Ningxia

    Institute of Scientific and Technical Information of China (English)

    杨晓娟; 靳军良; 王金保

    2014-01-01

    通过调查监测,宁夏农村面源污染的主要来源集中体现在化肥污染、农药污染、畜禽粪便污染、农膜污染及农村生活污染5个方面,但其影响和污染主要表现在对水环境和土壤环境的污染上。其中,对水环境的污染形式则是通过地表径流和地下淋溶把过剩的氮、磷、有机农药、无机盐等带到地表水和地下水中,致使地表水富营养化,破环水生态环境,鱼虾难以生存。对土壤环境的污染形式是通过农业投入品超量施入,在改变土壤物理性状、破环土壤通透性、造成土壤板结、粘结的同时,造成重金属离子和病原菌的积累,给农产品质量安全带来危害。%Based on an investigation and monitoring,the main sources of Ningxia rural non-point source pollution includes the five aspects of chemical fertilizer pollution, pesticide pollution, livestock and poultry manure pollution, agricultural plastic film pollution and rural domestic pollution. But the impact and pollution were mainly manifested in the pollution of water envi-ronment and soil environment. The form of pollution of the water environment was bringing excess nitrogen, phosphorus, or-ganic pesticides and inorganic salt to the surface water and underground water by the surface runoff and underground leach-ing,it caused the surface water eutrophication,the destruction of the aquatic ecological environment and made the fish and shrimp hard to survive. The form of pollution of the soil environment was changing the soil physical characteristic, destroying the soil permeability, causing soil compaction and bond,and causing the accumulation of heavy metal ions and pathogenic bacteria by excessive application of agricultural inputs, and it brought harms to the quality and safety of agricultural products.

  7. Demonstration and Effects of Best Management Practices Applied to Control of Rural Non-point Source Pollution in Poyang Lake Area%鄱阳湖区农村面源污染控制中最佳管理措施示范研究

    Institute of Scientific and Technical Information of China (English)

    万金保; 孙蕾; 刘峰; 汤爱萍

    2012-01-01

    Water environment security in rural areas around Poyang Lake has been threatened by increasingly severe non-point source pollution(NPS pollution).Combined with the features of NPS pollution in the areas,the structural best management practices(BMPs) are established in the demonstration area of Xingzi County to control the rural NPS pollution.Monitoring results show that in the monitoring period,the removal amounts of COD,TP and TN by the BMPs are 568.25,3.06 and 26.90 kg,respectively.Constructed wetland maintains the better and more stable pollutant removal capability and the removal amounts of COD,TP and TN per unit area are about 40,0.4 and 3.0 g/m2,respectively.The removal amounts of the pollutants per unit area by ecological ditch vary greatly.Ecological ditch has better removal ability per unit area than constructed wetland,but has poor stability.In consideration of the advantages of ecological ditch and tri-class surface flow constructed wetland(3-SFW),the plants suitable to ecological ditch are recommended to improve its stability in pollutant removal.%鄱阳湖区农村面源污染问题日益严峻,已威胁到湖泊的水环境安全。结合该区域农村面源污染自身的变化特征,在星子县示范区内构建结构性最佳管理措施BMPs(best management practices)系统对区域内农村面源污染进行控制。结果表明,在监测期间该BMPs系统对COD,TP和TN的削减量分别为568.25,3.06和26.90kg。其中人工湿地对COD,TP和TN的单位面积削减量约达40,0.4和3.0g/m2,有较稳定的污染物去除能力;生态沟渠对各污染物单位面积削减量变化较大,与人工湿地相比虽有更强的污染物削减能力,但稳定性较弱。结合生态沟渠和三级表面流人工湿地的特点,建议通过引入适宜的植物至生态沟渠提高其处理污染物的稳定性。

  8. 林带对太湖地区农业非点源的控制效应研究%Controlling effects of forest belts on non-point source pollution of agricultural lands in Taihu Lake area, China

    Institute of Scientific and Technical Information of China (English)

    陈金林; 侍璐璐; 张爱国

    2002-01-01

    in water, crops and underground of forest, the transfer and loss of N and P (main water pollutants) in faming ecosystem were studied, and the effects of forest belts on non-point source pollution of agricultural lands was analyzed. The results indicated that the transfer and loss of N and P vary with means of rotation, types of crops and the amount of fertilizer application. Buffering forest belts betweens farmlands and ditches can effectively stop and purify such elements as N and P in soil runoffs, thus controlling non-point source pollution of agricultural lands. When the width ratio of farmland to forest belt is 100 to 40, 50.05% losing N, 29.37% losing P can be absorbed by forest under rape-rice rotation and 30.98% N, 86.73% P can be absorbed by forest under wheat-rice rotation. When the width ratio of farmland to forest belt is 150 to 40, 33.37% losing N, 19.58% losing P can be absorbed by the forest under rape-rice rotation, and under wheat-rice rotation 20.65% lost N and 57.82% lost P can be absorbed. There is only some purification effect when the width ration of farmland to forest belt is 200 to 40. Based on model of buffering forest belts, the width ratio of farmland to forest is determined between 100 to 40 and 150 to 40, because it not only can purify water, but also occupy less farmland. It is suggested that Poplars, with the characteristics of fast-growing and high value, are suitable to be planted as shelter-forest in Taihu Lake Watershed.

  9. 苏州市东山镇池塘养蟹面源污染现状及控制%Current status and control strategies of non-point source pollution from pond aquaculture in Dongshan town of Suzhou

    Institute of Scientific and Technical Information of China (English)

    宋学宏; 郭培红; 孙丽萍; 朱江

    2011-01-01

    结合全国污染源普查活动,以苏州市养殖老区东山镇养殖池塘为典型,调查其现行养殖模式、经济效益、养殖污染状况.调查结果显示,东山镇现有养殖面积为2253.33hm2,主养品种为河蟹.1个养殖周期中,养殖池塘通过沟渠排入外界的TN、TP含量分别平均为21.25kg/hm2、2.34kg/hm2;密度为9000只/hm2的低密度养殖池外排的TN含量为16.79kg/hm2,而无TP排出.分析苏州地区池塘养殖产生水环境污染的主要原因,从池塘管理体制改革、科学生态养殖技术及养殖尾水处理等方面探讨池塘养殖污水零排放技术,为苏州市养殖业面源污染的控制提供对策和措施.%Combining with national census of pollution sources and using large-scale pond aquaculture in Dongshan town in Suzhou as a case study, the current cultural modes, economic benefits, and aquaculture pollution were surveyed. The results showed that there were 2 253.33 hm2 of aquaculture in Dongshan town, and the Chinese mitten crab was the main specie. In a single aquaculture period, the discharged total nitrogen (TN) and total phosphorus (TP) from pounds to outside through canals and ditches were on average 21.25 kg/hm2 and 2.34 kg/hm2 respectively. However, in a lowdensity crab raising pond of 9000 ind/hm2, the discharged TN was 16.79 kg/hm2, and no TP was found to be discharged.Based on the case study, the main reasons for the water environment pollution by pond aquaculture were analyzed, and the zero discharge technologies for the waste water of pond aquaculture were discussed from aspects of the management system reform for pond, the scientific and ecological raising technique, and the aquaculture wastewater treatment. The possible strategies and countermeasures for controlling the aquacultural non-point source pollution in Suzhou were also provided.

  10. Spatial-temporal Variation of Agricultural Non-point Source Pollution Based on GIS Technology in Guangdong Province, China%基于GIS的广东省农业面源污染的时空分异研究

    Institute of Scientific and Technical Information of China (English)

    叶延琼; 章家恩; 李逸勉; 李韵; 吴睿珊

    2013-01-01

    近年来,广东省化肥、农药等农用化学品的投入量大增,农业面源污染问题日趋严重.采用历年统计数据以及GIS技术,对广东省农业面源污染的时空分异性进行了分析.结果表明,全省化肥、农药使用量均呈逐年增加态势,化肥使用量由1990年的162.41万t增加到2010年的237.29万t,农药由1990年的7.95万t增加到2010年的10.44万t.畜禽粪尿排放量则由2000年的339.34亿t减少到2010年的222.08亿t;在空间分布上,全省化肥投入强度超过400 kg· hm-2的县(市)由1995年的35个增加到2010年的61个,粤西、粤东全部,珠三角大部以及粤北部分地区均属化肥严重污染区,其原因主要在于对农作物产量增加的追求.农药投入强度超过30 kg· hm-2的县(市)由1995年的10个增加到2010年的27个,这主要是由于广东的气候条件及种植结构改变造成的.畜禽粪尿排放污染情况相对较好,但因粤西的雷州半岛地区以水稻生产为主,大牲畜牛的养殖规模较大,因此畜禽粪尿重度污染主要集中在这一地区.GIS空间分析的运用,能直观地反映各地区农业面源污染的时空动态变化情况,为有针对性地开展污染治理提供依据.%Agriculture non-point source pollution (ANSP) is becoming a serious environmental concern in Guangdong Province due to the increased applications of agricultural chemicals such as fertilizers and pesticides in recent years. This study investigated the spatial-temporal variation of ANSP using the historical statistical data and GIS technology. Results showed that the fertilizer, pesticide and other agrochemical applications in Guangdong Province were increased and reached quite a high level in order to secure food supply. The total amount of chemical fertilizer application increased from 1.62x1061 in 1990 to 2.73x1061 in 2010 and the total amount of pesticide application elevated from 7.95X1041 to 10.04X1041, while the total amount of animal manure

  11. Non-Point Pollution from Crop Production: Global,Regional and National Issues

    Institute of Scientific and Technical Information of China (English)

    D.NORSE

    2005-01-01

    China is now the world's largest producer and user of industrial fertilizers and manures. Consequently China plays a substantial role in global N cycle dynamics and in man's disruption of the nitrogen cycle though there are still significant uncertainties about the size and importance of emission and leaching rates. A major cause of 1China's global role is the overuse of nitrogen fertilizers, which is most serious with intensive vegetable production where application rates can be up to 50% greater than crop needs, but is also a problem with wheat, rice and maize.China's overuse of nitrogen fertiliser over the past 10-20 years has resulted in non-point source (NPS) pollution from crop production becoming a major cause of water pollution, and the situation is projected to get worse. In contrast, water pollution from point sources such as intensive livestock production and urban or industrial development is being brought more under control. The consequences for air pollution are equally serious. Emissions of nitrous oxide from fertilizers and manures may be so large that China could be responsible for 25-30% of global emissions of this damaging greenhouse gas and of the global warming resulting from it.The main national and local issues relate particularly to low fertilizer use efficiency and the losses of ammonia and NOx that lead to acid precipitation, and leaching and run-off losses that result in high nitrate levels in groundwater and eutrophication of rivers and lakes. The reasons why farmers overuse nitrogen fertilizer are complex and not fully understood. They involve agro-climate differences between provinces and counties, farming systems and farm income structures. Although there is a wide range of institutional and technological improvements that can greatly reduce this overuse rapid progress in reducing NPS is unlikely.

  12. NPS career forward

    OpenAIRE

    McLay, Deidre

    2014-01-01

    "NPS provided a critical link in my career from warfighting operator to strategic thinker and reasoner. I found the education at NPS to be superior in every respect, and I enrolled in as many classes as was physically possible during my time there so that I could take advantage of the expertise and resources that were available. My NPS education, as well as the Strategic Group XIX fellowship formulated many of the current initiatives for my Joint UAS Concepts team." US Navy (USN) author

  13. “双重失灵”视角下我国农业面源污染的法律规制%Legal Regulation of the Agricultural Non-point Source Pollution in the Perspective of “Double Failure”

    Institute of Scientific and Technical Information of China (English)

    祝创杰

    2016-01-01

    来自化肥、农药、农膜、畜禽养殖等污染源的农业面源污染已经成为我国水污染的最大污染源。现行立法对农业面源污染的规制在指导思想、具体内容和立法技术等方面仍存在一些不足,而这些不足又是忽略或缺乏经济法的思维方式所致。我国农业面源污染恶化是市场失灵和政府失灵共同作用的结果。克服“双重失灵”,有效规制面源污染,可以通过完善环境税收体系,增设生态税、建立排污权交易制度等经济法的调整手段实现。%The Agricultural Non-point Source Pollution (ANPSP),which is caused by pollution source like chemical fertilizers,pesticides,agricultural film and livestock breeding,has already be-come the largest source of pollution of our country's water pollution.The regulation of The Agricultural Non-point Source Pollution given by current legislation still has some deficiencies in guiding ideology, concrete content and legislative technique.This is caused by neglecting or lacking the thinking mode of economic law.The root cause of the deterioration of The Agricultural Non-point Source Pollution in China is the combined action of market failure and government failure.The task of overcoming “double failure”and controlling The Agricultural Non-point Source Pollution can be finished by using adjust-ment methods of economic law.The methods may include improving the environmental tax system, adding ecological taxation and establishing emission trading system.

  14. Optimization of Policy and Management Service System on Control of Rural Non-Point Source Pollution-A Case Study of the Rural Area of Shanghai%农村面源污染治理的政策与管理服务体系优化——以上海郊区农村为例

    Institute of Scientific and Technical Information of China (English)

    黄文芳; 沈哲

    2012-01-01

    It is a common view that control of rural non-point source pollution is not only a technology issue, but also a management system issue. So hard currently it has become a hot spot and a hard nut as well to probe and consummate the policy, management and service system for control of rural non-point source pollution. Selecting the rural suburbs of Shanghai for case study, the status quo of the rural non-point source pollution and its management have been analyzed and summarized. While affirming the effect of the current work on pollution management, the limitations of the work are discerned and it was pointed out that inharmony between the agriculture development policy and the environmental protection policy and the lag of the management mechanism and service system behind the change in the rural development model are the basic causes. To counter the major contributors of the rural non-point source pollution, i. e. chemical fertilizers, pesticides , livestock excretes and rural domestic sewage, countermeasures and suggestions are presented for management of the pollution from the aspects of modification of the agricultural subsidization system, reconfiguration and implementation of the rural multi-value system and consummation of agricultural production service system.%农村面源污染控制不仅是技术问题,也是管理体制与机制问题,探讨并完善农村面源污染治理的政策、管理与服务体系已成为当前研究的热点与难点.以上海郊区农村为例,梳理总结农村面源污染及其控制现状;在肯定当前治理效果的同时,识别其局限性,指出农业政策与环境保护政策的不协调、农村发展模式转变下制度与服务体系的相对滞后是根本原因;针对当前农村面源污染重要的贡献者——化肥、农药、畜禽粪便以及农村生活污水,分别从改革农业补贴制度、重构实现农村多重价值的制度以及完善农业生产服务体系等方面给出了治理的对策与建议.

  15. New psychoactive substances (NPS) on cryptomarket fora: An exploratory study of characteristics of forum activity between NPS buyers and vendors.

    Science.gov (United States)

    Van Hout, Marie Claire; Hearne, Evelyn

    2017-02-01

    The continual diversification of new psychoactive substances (NPS) circumventing legislation creates a public health and law enforcement challenge, and one particularly challenged by availability on Hidden Web cryptomarkets. This is the first study of its kind which aimed to explore and characterise cryptomarket forum members' views and perspectives on NPS vendors and products within the context of Hidden Web community dynamics. An internal site search was conducted on two cryptomarkets popular with NPS vendors and hosting fora; Alphabay and Valhalla, using the search terms of 40 popular NPS in the seven categories of stimulant/cathinone; GABA activating; hallucinogen, dissociative, cannabinoid, opioid and other/unspecified/uncategorised NPS. 852 identified threads relating to the discussion of these NPS were generated. Following exclusion of duplicates, 138 threads remained. The Empirical Phenomenological Psychological method of data analysis was applied. Four themes and 32 categories emerged. 120 vendors selling NPS were visible on Alphabay, and 21 on Valhalla. Themes were 'NPS Cryptomarkets and Crypto-community interest in NPS'; 'Motives for NPS use'; 'Indigenous Crypto Community Harm Reduction'; and 'Cryptomarket Characteristics underpinning NPS trafficking', with two higher levels of abstraction centring on 'NPS vendor reputation' and 'NPS transactioning for personal use'. NPS cryptomarket characteristics centred on generation of trust, honesty and excellent service. Users appeared well informed, with harm reduction and vendor information exchange central to NPS market dynamics. GABA activating substances appeared most popular in terms of buyer interest on cryptomarkets. Interest in sourcing 'old favorite' stimulant and dissociative NPS was evident, alongside the sequential and concurrent poly use of NPS, and use of NPS with illicit drugs such as MDMA. Continued monitoring of new trends in NPS within Surface Web and cryptomarkets are warranted. A particular

  16. Advances on agricultural non-point source pollution and the control in regions around Hung-tse Lake%环洪泽湖区域农业面源污染特征及控制对策

    Institute of Scientific and Technical Information of China (English)

    徐勇峰; 陈子鹏; 吴翼; 朱咏莉; 李萍萍

    2016-01-01

    Hung⁃tse Lake, located in the northwest of Jiangsu Province, is the fourth largest freshwater lake in China. It is also one of the key hinge lakes in the east route of the south⁃to⁃north water transfer project. However, the water quality for the lake was worse than grade Ⅴ according to the Environmental Quality Standard of Surface Water of the People�s Republic of China. Non⁃point source pollution around the lake was considered one of the main reasons. In the present study, the feature, sources, inducement, control and management of agricultural non⁃point pollution around the lake were reviewed. We held that the unreasonable application of chemical fertilizer was the largest source of the pollution. Aquaculture pollution was substantially increasing due to the facts of the ineffective management to enclosure culture and the abuse of exogenous diets. In addition, the emissions of livestock and poultry breeding, rural sewage and unordered waste management were the main contributors of the pollution. Therefore, the control strategies for the agricultural non⁃point source pollution in the regions should focused on ① developing ecological agriculture with reasonable applications of fertilizer and pesticides,②integrating and optimizing the aquaculture,③improving the treatment of rural sewage and recycling of agricultural wastes,④ reducing pollution by enhancing the land use among farmlands, woodlands and wet⁃lands.%洪泽湖是位于江苏省西北部的我国第四大淡水湖泊,同时是南水北调东线工程的重要节点湖泊。然而,目前洪泽湖水质总体状况仍呈下降趋势,其中面源污染是重要原因之一。笔者对环洪泽湖地区农业面源污染的特点、来源与诱因以及控制技术等进行系统评述,分析认为:不合理的化肥施用是该区域第一大面源污染来源;围网养殖区无序规划及大量外源性饵料的投加,使得水产养殖的污染排放呈现增加趋

  17. The Empirical Analysis of EKC on Agricultural Non-Point Source Pollution in Chongqing%重庆市农业面源污染源的 EKC实证分析

    Institute of Scientific and Technical Information of China (English)

    刘志欣; 邵景安; 李阳兵

    2015-01-01

    Based on the Environmental Kuznets Curve theory and the data from 2000-2012 of Chongqing , five indexes ,w hich are related to the agricultural non‐point source pollution ,are selected as variances and the Environmental Kuznets Curve model are established to analyze the relationship between agricultural non‐point source pollution and the agricultural economic growth .Meanwhile ,use the software of Eviews 7 .0 and SPSS 17 .0 to estimate the parameters .The conclusions can be drawn as follows :1) The chemical fertilizer application level ,the pesticide application level and the emission density of crop residues exhibited typical inverted‐U shaped curve relations with the per capita agricultural net income of farmers .And the chemical fertilizer application level ,pesticide application level and the emission density of crop residues have reduced with the agricultural economic grow th .2) During the study period ,the agricultural films ap‐plication level exhibited liner relations with the per capita agriculture net income of farmers .With the grow th of agricultural economy ,the environmental pressure caused by agricultural films will be further in‐creased .3) The pig droppings equivalent emission density of livestock and poultry manure with the per ca‐pita agricultural net income of farmers exhibited “N”shaped curve characteristics ,the curve exists two in‐flection points .Currently it’s on the right side of the second inflection point and it indicates that the envi‐ronmental pressure caused by livestock and poultry manure pollution will deteriorate again with the devel‐opment of agricultural economy .The government should take effective measures to strengthen the agricul‐tural non‐point source pollution control .%基于环境库兹涅茨曲线理论,根据重庆市2000-2012年的时序数据,选取5个与农业面源污染有关的指标作为污染变量,分析农业面源污染与农业经济增长的关系,并利用EVIEWS7.0

  18. Review on super absorbent polymer application for improving fertilizer efficiency and controlling agricultural non-point source pollutions%高吸水树脂保水剂提高肥效及减少农业面源污染

    Institute of Scientific and Technical Information of China (English)

    廖人宽; 杨培岭; 任树梅

    2012-01-01

    Super absorbent polymer (SAP) is a new type of multifunctional material in drought resistant and pollution control. SAP can quickly absorb water and fertilizer for increasing soil crumb structure and porosity, restraining soil evaporation, promoting crop physiological function and reducing fertilizer loss, so as to result in significant improvement of water and fertilizer use efficiency and marked reduction in fertilizer pollution. In recent years, SAP has been widely used in agricultural nonpoint source pollution control as a chemical product of absorbing and storing water and fertilizer. The paper expounds its acting mechanism and efficiency in controlling pollution, introduces the researches on its main antifouling function, and emphatically summarizes the researches of SAP application in agricultural production and the development of agricultural antifouling absorbent agent. In the end, the problems existing in the field are discussed and the future research fields are proposed.%高吸水树脂(保水剂)是一种新型多功能抗旱、防污材料,能够快速吸持水肥,增加土壤团粒结构和孔隙度,抑制土面蒸发,提升作物生理机能,减少养分淋失,从而达到提高水肥利用效率和减少肥料污染的作用.近年来,保水剂在农业面源污染防治方面得到了越来越广泛的应用.该文阐述了保水剂的防污作用机理及效能,介绍了保水剂主要防污作用方面的研究,重点对保水剂在农业生产上的防污应用及农用防污型保水剂的研制进行了综述,最后分析了保水剂在农业防污应用和研究过程中存在的问题,提出了保水剂未来进行防污应用的研究方向.

  19. Characterization of non point source pollutants and their dispersion ...

    African Journals Online (AJOL)

    EJIRO

    African Journal of Environmental Science and Technology Vol. 5(2), pp. ... wet season increases the concentrations of ammonia, phosphorus, nitrites and nitrates. Ammonia .... sampling coordinates were stored in a GPS and later traced during ..... threats, impacts and conservation strategies in the African Great,. Lakes.

  20. 基于改进USLE模型的北运河流域非点源污染潜在敏感区分析%Potential Sensitive Areas Analysis of Non-Point Source Pollution in North Canal Basin based on Improved USLE Model

    Institute of Scientific and Technical Information of China (English)

    靳美珠; 张晓惠; 袁雪竹

    2012-01-01

    Based on the universal soil loss equation (USLE), the model of demarcation of sensitive areas of the non-point pollution in North Canal basin is developed. With the database of TM remote sensing images, digital elevation model (DEM), and the map of soil use type in research area in 2009, distribution map of sensitive areas of non-point source in North Canal basin is produced by the model. Base on the simulation, the three risk areas (high, medium and low) and safe areas are identified. Results show that improved model can be effectively used in the analysis of sensitive areas of non-point source pollution.%基于通用土壤流失方程(USLE)建立北运河流域非点源污染敏感区的划分模型,以2009年研究区域TM遥感影像、数字高程模型(DEM)、研究区土壤利用类型图等为数据基础,在GIS9.3和ERDAS9.2平台上通过对各模型因子进行栅格运算生成北运河流域非点源污染潜在敏感区分布图,在此基础上划分了高、中、低三个风险区以及安全区.鉴于我国与美国不同的自然条件,以及土壤流失与固体垃圾的扩散之间所存在的差异,在引进和应用该模型时,对方程中各因子的算法和参数做了一定的调整和改进,结果表明改进后的模型可以有效的运用于非点源污染敏感区的分析.

  1. Today@NPS / July 2016

    OpenAIRE

    NPS Public Affairs Office

    2016-01-01

    Today@NPS showcases some of the speakers, conferences, experiments, lectures and other events that take place at the Naval Postgraduate School Contents: July 1, 2016: NPS Leadership Share Life Primers with Summer STEM Interns; July 5, 2016: Summer Quarter Welcomes Largest Incoming Class of the Year; July 6, 2016: NSAM Welcomes New Students With MWR Leisure Fair; July 7, 2016: Bowman Scholars Make the Rapid Transition to Graduate Studies; July 8, 2016: NPS Legal Details the Basics of Protec...

  2. ASSESSMENT AND CONTROL OF NITROGEN EMISSION FROM AGRICULTURAL NON-POINT SOURCE IN THE URBAN AGGLOMERATION IN THE MIDDLE-LOWER YANGTZE RIVER BELT%长江中下游城市群农业面源污染氮排放评价及调控

    Institute of Scientific and Technical Information of China (English)

    赖敏; 王伟力; 郭灵辉

    2016-01-01

    Nitrogen is an important component of protein and essential element for the growth of aquatic biomass. Excessive nitrogen input to natural water bodies leads to huge ecological pressure and environmental pollutions such as eutrophication. As point source pollution got effective control in the socio-economic system, agricultural non-point source pollution has become the main cause of eutrophication. Quantifying and regulating the agricultural non-point source pollution emissions throughout the entire socio-economic system is crucial to mitigate or avoid pro-ducing water pollution. Based on the inventory analysis method and emission coefficient method, this paper calcu-lated the nitrogen emission from the agricultural non-point source for the Urban Agglomeration in the Middle-Lower Yangtze River Belt. The results showed that the total nitrogen emission from the agricultural non -point source in the whole area was 128. 27 × 104 t in 2011, of which agricultural emission accounted for 58. 92%, and e-mission from livestock and poultry was 33 . 53%. The sensitivity analysis method and scenario analysis method were then applied to simulate the emission situation during 2011 to 2020 and 2020 to 2030 . Some conclusions were drawn as follows:Under scenario 1, if more stringent pollution control efforts were not implemented, the nitrogen e-mission from the agricultural non-point source would increase 16 . 29% during 2011 to 2020 , and 18 . 78% during 2020 to 2030. Under scenario 2 and scenario 3, by contrast, the total nitrogen emission of the four urban agglomer-ations would be 15% fewer in 2020 than in 2011 , and 25% fewer in 2030 than in 2011 . The nitrogen emission re-duction effect in scenario 2 was better than that in scenario 3;Scenario 3 was considered to be more conducive to couple the relationship between regional economic growth and environmental protection. Finally, some recommen-dations were put forward to regional emission reduction, including reducing

  3. Source apportionment and spatial heterogeneity of agricultural non-point source pollution based on water environmental function zoning%基于水环境功能区划的农业面源污染源解析及其空间异质性

    Institute of Scientific and Technical Information of China (English)

    钱晓雍; 沈根祥; 郭春霞; 顾海蓉; 朱英; 王振旗

    2011-01-01

    Taking township as basic unit, the inventory analysis method and equivalent standard method were conducted to determine the discharge amount of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) from different types of agricultural non-point sources, including chemical fertilizer application, animal manure application, crop straws, animal husbandry, aquaculture and rural sewage. The impact of agricultural non-point source pollution (ANPSP) on water environment and its spatial heterogeneity were analyzed based on water environmental function zoning at the scales of county and town. The results indicated that, the absolute discharge amount of COD, TN and TP from ANPSP were 4.42× 104,1.13× 104, 0.44× 104 t/a respectively, and the discharge amount in equivalent standard were 0.16× 104, 0.93 × 104, 1.65× 104 m3/a respectively. The primary pollution source was animal husbandry, which load ratio in equivalent standard reached 66.31%, and the primary pollutant was TP, which load ratio in equivalent standard reached 60.32%. Discharge concentration of COD, TN and TP caused by ANPSP in different districts were in the range of 4.16-40.91, 1.30-8.71, 0.23-4.94 mg/L respectively, while the average water quality index ranged from 0.67 to 5.91. From the point view of town-based pollution stress, the pollution extent of the towns in South Shanghai and Chongming Island was much higher, which had greater agriculture productive value and located near the water conservation area with stricter water quality standards.%通过清单分析方法和等标污染负荷法,以乡镇为单元研究了上海市化肥施用、有机肥施用、农作物秸秆、畜禽养殖、水产养殖、农村生活污水等农业面源污染来源化学需氧量(COD)、总氮(TN)、总磷(TP)等污染物的排放量及其贡献率,并根据各区域水环境功能区划分别在区县尺度和乡镇尺度分析了农业面源污染程度及其区域分布.结果表明,上

  4. 东江源山地果畜结合区面源污染生态化控制模式与效果分析%Study on ecological control mode of non-point source pollution from the system of orchard linked with animal husbandry in the valley of Dongjiang River Headwater

    Institute of Scientific and Technical Information of China (English)

    席运官; 刘明庆; 王磊; 汪贞; 李德波; 王宏燕

    2013-01-01

    The paper studied the structure and functions of pig-biogas-fruit-fish eco-agricultural mode in the headwater of Dongjiang River based on the Xinglin farm in Dingnan County, analyzed the controlling effect in quality and quantity to the water pollution nutrition through source controlling, drainage reducing and cleaning, and tested the draining water of Xinglin farm showing that draining water quality was better than the Ⅳ level standard of surface water. It proved that this model had the features of opearational, extentional and low input, and could be the important mode for controlling the non-point source pollution in the headwater of Dongjiang River.%  分析东江源山地果畜结合区“猪-沼-果-鱼”生态农业模式的结构与功能,定性和定量分析农庄采用生态化技术对水体污染物控源、减排、净化的效果,跟踪采样分析农庄排水水质,结果表明,农庄排水全年优于地表水Ⅳ类水标准,该模式兼具可操作、可推广、低投入特性,是东江源控制农业面源污染的重要模式。

  5. 农业面源污染治理的政策效用评估以江苏省海安县的测土配方施肥推广为例%Agricultural Non-point Source Pollution Controlling Policy's Utility Evaluation Based on Environmental Cost:A Case Study of Soil Testing and Fertilizer Recommendation Technology in Hai'an County

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    为衡量农业面源污染治理政策的作用效果,文章基于环境成本的视角,探索了农业面源污染治理政策效用评估的一般方法与步骤。首先,结合农业化肥的环境污染特点,利用输出系数模型以实现对区域内农业化肥造成的总氮TN、总磷TP排放量的合理测算。然后,建立了基于能值分析法的环境成本估计模型,并结合经济成本和经济收益,构建了基于成本-收益分析的农业面源污染治理政策效用评估的一般模型。最后,以江苏省海安县的测土配方施肥技术的推广为例,对海安县测土配方施肥节本增收效果进行综合评价,并探索了江苏省实现测土配方施肥全覆盖时的总体效果,为面源污染治理政策的评估提供思路。%In order to assess the policy effect of controlling agricultural non-point source pollution ,this paper ,based on the perspec‐tive of environmental cost ,explores the general methods and steps for utility evaluation of agricultural non-point source pollution's treatment measures .First of all ,combined with the characteristics of environmental pollution caused by agricultural fertilizer ,this paper chooses a rational export coefficient model to calculate total nitrogen (TN) ,total phosphorus (TP) emissions caused by agri‐cultural fertilizer in the region .Then ,targeted at soil testing and fertilizer recommendation technology ,this paper constructs envi‐ronmental cost estimation model based on energy analysis and general utility evaluation model based on cost-benefit analysis .Finally , this paper takes Hai'an County's promotion of soil testing and fertilizer recommendation in Jiangsu Province for example ,the synthe‐sized evaluation of the technology is achieved in saving costs and increasing income .Further ,this paper explores the beneficial se‐quence when the technology is spread to the whole Jiangsu Province ,which will provide ways to better choose

  6. 基于排水过程分析的水稻灌区农田面源污染模拟%Simulation of agricultural non-point source pollution from paddy rice irrigation district based on analyses of drainage processes

    Institute of Scientific and Technical Information of China (English)

    陈会; 王康; 周祖昊

    2012-01-01

    对前郭灌区主要面源污染物迁移、转化及汇集过程开展了2a的系统试验与监测,模拟了灌区面源污染水质水量过程,分析了灌区农田面源污染形成机制.水均衡测定结果表明,灌区排水主要由灌溉退水、稻田地表弃水和稻田渗流排水3部分组成,采用马斯京根法和连续分段马斯京根法能够有效地模拟各级排水沟道的排水过程.主要面源污染物随水体发生迁移及掺混,采用一级动力学方法描述污染物转化过程,模拟的灌区水质水量过程与实际过程符合较好,稻田地表退水主要影响水稻抽穗前的面源污染入河过程,而渗流排水则在抽穗后灌区排水水质中起主要作用.结果表明水稻灌区中地表排水和稻田渗漏排水对于面源污染过程起主要作用.%The transport and transformation processes of non-point source pollutions form paddy rice field to the main drainage canal through lateral and branch drainage canals were monitored in the Qianguo irrigation district during the rice growing seasons in 2009 and 2010. Water balance were measured in lateral canal in the controlled irrigation region. Results showed that the drainage water were composed of the rice field surface returned water, the irrigation returned water and the seepage from rice field to the drainage canals. Drainage processes in branch and main canal were simulated using the Muskingum method and the Muskingum segmentation flow routing method, respectively. The transport processes of chemical concentrations were determined by the mix and convection of water flow and the transformation processes were described using the first order kinetic equation. Drainage processes and contaminant concentration simulated showed good agreements with the measured values. The returned water and seepage from rice field played key roles in the process of agricultural non point pollution into the river. This research suggested the surface drainage and seepage

  7. 引导农户施肥行为在农业面源污染治理中的影响--基于中英项目调查分析%The Effects from Guidance of FarmersˊBehavior for Fertilizer Using in the Regulation of Agricultural Non -point Source Pollution:Based on the Survey of China -UK Educational Program

    Institute of Scientific and Technical Information of China (English)

    华春林; 陆迁; 姜雅莉

    2015-01-01

    Based on the implementation of the China -UK educational program,this paper empirically analyzes the effects from guidance of farmersˊbehavior for fertilizer using with the survey data of 331 farmers in Yangling Demonstration Zone and Wugong County of Shaanxi Province.The result indicates that the amount of farmersˊfertilizer using is significantly re-duced by correct guidance.During the process of constructing the governance micro -mechanism of agricultural non -point source pollution,it is necessary to seriously consider the guiding function of educational program.%基于中英项目实施情况,利用陕西省杨凌示范区及泾阳县331份农户实地调查数据,实证分析引导农户化肥施用行为在污染治理中的影响。分析结果表明对农户化肥施用行为的正确引导会减少化肥量的投入,最终影响我国农业面源污染的治理效果。因此,在构建我国农业面源污染微观治理机制时,要重视具有引导农户生产行为作用的治理手段。

  8. 基于“压力-响应”机制的江苏省农业面源污染源解析及其空间特征%Spatial Distribution Characteristics and Source Origin of Agricultural Non-point Source Pollution in Jiangsu Province Based on Pressure-response System

    Institute of Scientific and Technical Information of China (English)

    陆尤尤; 胡清宇; 段华平; 卞新民

    2012-01-01

    运用清单分析、等标负荷和聚类分析等方法,对江苏省农业面源污染源、影响因子、空间分布特征等进行了分析与评价.结果表明:江苏省农业面源污染化学需氧量(COD)、全氮(TN)、全磷(TP)绝对实物排放量分别为155.23×104 t/a、62.34×104 t/a、9.05×104t/a,相应的绝对等标排放量分别为7.76×104 t/a、62.34×104 t/a、45.23×104t/a.农业面源污染造成的COD、TN、TP的平均排放浓度分别为6.25 mg/L、2.53mg/L、0.36 mg/L,排放浓度地区差异显示苏北>苏中>苏南.农业面源污染综合水质指数显示,江苏省均值为2.10,达到中度污染水平.其中徐州、连云港、宿迁处于严重污染状态;淮安、盐城、泰州处于中度污染状态;南通、扬州处于轻度污染状态;南京处于警戒状态;无锡、常州、苏州、镇江处于安全状态.主要污染物依次是TN、TP,其贡献率分别为54.71%和38.86%;主要污染源依次是化肥施用、畜禽养殖、人粪尿、水产养殖,其贡献率分别为30.75%、24.94%、16.85%、15.28%;在国土面积、农业产值和农村人口几大因子中,农业产值是与污染物排放量相关性程度最高的因子.通过对”压力-响应”的表征量进行聚类分析得出江苏省农业面源污染的空间分布特征,其压力和响应基本一致.%The primary source, influence factors, and spatial distribution characteristics of agricultural non-point source pollution (NPSP) in Jiangsu Province were analyzed by inventory analysis, equivalent standard load, and cluster analysis methods. Results showed that the absolute real emissions of COD, TN and TP from NPSP were 155.23× 104t/a, 62.34×l04t/a and 9.05×l04t/a respectively. And the related standard emissions were 7.76×104 t/a, 62.34×104t/a and 45.23×104 t/a. The average concentrations of COD, TN and TP emitted from NPSP were 6.25 mg/L, 2.53 mg/L, 0.36 mg/L, respectively. The concentrations of these

  9. 考虑面源污染的中国苹果全要素生产率及其空间集聚特征分析%Total factor productivity of apple industry in China considering non-point source pollution and its spatial concentration analysis

    Institute of Scientific and Technical Information of China (English)

    冯晓龙; 霍学喜

    2015-01-01

    Apple industry has become a major industry of rural economy in most of the north region in China, which plays an important role on the adjustment of agricultural structure, the increase of farmer’s income and the export. But overall, the development of apple industry still has belonged to the traditional production style depending on fertilizer and pesticide; on one side it has brought high economic benefits for farmers, and on the other side it has been the main reason to agricultural non-point source pollution. There is no doubt that this production style has exacerbated the contradiction between the development of apple industry and the ecological environment in China. Therefore, in order to achieve the sustainable development of apple industry, the transformation of the development mode of apple industry, the improvement of resource use efficiency and the effective control of non-point source pollution in apple production should be taken into account urgently. And to achieve these goals are closely dependent on the contribution of apple total factor productivity to industry development, the reasonable estimation of apple total factor productivity, and thus the guide of the correct policy. Using the unit investigation and evaluation model, this paper calculates the non-point source pollution from apple industry, which is taken as non-ideal output and integrated into the model of total factor productivity, then analyzes the total factor productivity of apple industry under the constraint of environment of 21 provinces in China from 1994 to 2013 by applying the Malmquist-Luenberger productivity index, and finally tests the spatial autocorrelation of total factor productivity of apple industry by using Moran’s index. The results show as follows: 1) Apple total factor productivity index under the constraint of environment is lower than that without this constraint, implying that environment pollution has obvious negative effects on apple productivity in China

  10. 日本福岛第一核电站事故源项及后果评价%Fukushima Daiichi NPS Accident Source Term and Consequence Assessment

    Institute of Scientific and Technical Information of China (English)

    王海洋; 黄树明; 王晓霞; 尤伟; 米爱军; 张普忠

    2011-01-01

    根据已有的日本福岛第一核电站相关资料,利用美国核管理委员会《轻水堆核电厂事故源项》中的假设条件,计算出事故后安全壳内的放射性源项,综合考虑各种不确定性因素,得出较为保守的环境释放源项。采用美国核管理委员会RG 1.4中大气扩散模式的假设计算大气弥散因子,并应用ICRP 71号出版物F、GR 12号报告等资料中的剂量计算模式及剂量转换因子进行了事故剂量后果的估算、分析和评价。%Conservative source term of radioactive release to the environment is calculated after the accident at Fukushima Daiichi nuclear power station based on updated informational materials and the assumptions made in NUREG-1465 by U.S.Nuclear Regulatory Commission(U.S.NRC),with the consideration of the various uncertainties.And a series of atmospheric dispersion factors are obtained from U.S.NRC Regulatory Guide 1.4(RG 1.4).Finally,this paper provides calculation of the accidental dose,which is analyzed and assessed specifically,using the models and parameters in ICRP Publication 71,FGR 12 and so on.

  11. Information Engineering of the curricular Officers’ Segment of a Unified Student Academic Database System for NPS

    Science.gov (United States)

    1991-09-01

    HOUSING OCCUPANT SECTION-NUMBER SPLIT SECTION PROPOSED NPS DEGREE ACCREDITATION STATUS NPS MAJOR COMMISSIONING SOURCE STUDY SPACE LOCKER NUMBER DATE OF...ACCREDITATION STATUS NPS MAJOR - COMMISSIONING- SOURCE STUDY SPACE LOCKER NUMBER DATE OF BIRTH PLACE OF BIRTH CITY PLACE OF BIRTH STATE DATE REPORTED ABOARD...LAMESA HOUSING OCCUPANT SECTION -NUMBER SPLIT SECTION - PROPOSED NPS DEGREE ACCREDITATION STATUS NPS MAJOR - COMMISSIONING- SOURCE STUDY SPACE LOCKER

  12. Evaluating Urbanization Impacts from Non-Point Stormwater Runoff using Geospatial Analysis

    Science.gov (United States)

    Zivkovich, B. R.; Mays, D. C.

    2015-12-01

    Sediments, nutrients and other chemical impairments caused by urbanization continue to deteriorate natural ecosystem processes, resulting in the current degraded state of urban surface waters. Understanding non-point source impacts on these natural ecosystems has become a prevalent topic in sustainable urban infrastructure design as efforts to restore the urban hydrologic regime continue to drive engineers, planners, and environmentalists to develop optimal design practices for rapidly expanding built environments. To best understand how and where these impairments are received, the U.S. Environmental Protection Agency and other organizations have adopted urban runoff programs to identify contributions from non-point sources. This presentation provides a geospatial analysis method for identifying non-point source watersheds, and associated sub-basins, that contribute the highest loads of pollutants to receiving urban streams and lakes. This method, using a form of linear matrix inversion, is an area-averaged weighting method for non-point pollutants that corresponds to a geospatial land cover analysis. This two-phase analysis can be used to aid all parties in understanding how different land use types affect urban stream systems and processes. Optimal locations for water quality features (i.e., best management practices) can be evaluated in order to reduce, capture, and treat stormwater runoff as close to the source as possible. These best management practices have the ability to operate most effectively when located properly, because their ability to act as a minor treatment and prevention system is of great important for the restoration of the urban hydrologic regime.

  13. Inside NPS, December 2010 [video

    OpenAIRE

    Naval Postgraduate School (U.S.)

    2010-01-01

    TNT Camp Roberts,Battlefield Medic,Paul Stockton. "Inside NPS" is a monthly show that features a wide range of university news, from research projects and student efforts to distinguished visitors and faculty honors. The monthly 30-minute broadcast is aired on the Pentagon Channel and can be seen locally on Access Monterey Television. With Host Alan Richmond.

  14. Assessing the Long-Term Hydrologic Impact of Land Use Change Using a GIS-NPS Model and the World Wide Web

    Energy Technology Data Exchange (ETDEWEB)

    Bhaduri, B.; Engel, B.; Harbor, J.; Jones, D.; Lim, K.J.

    1999-09-22

    Assessment of the long-term hydrologic impacts of land use change is important for optimizing management practices to control runoff and non-point source (NPS) pollution associated with watershed development. Land use change, dominated by an increase in urban/impervious areas, can have a significant impact on water resources. Non-point source (NPS) pollution is the leading cause of degraded water quality in the US and urban areas are an important source of NPS pollution. Despite widespread concern over the environmental impacts of land use changes such as urban sprawl, most planners, government agencies and consultants lack access to simple impact-assessment tools that can be used with readily available data. Before investing in sophisticated analyses and customized data collection, it is desirable to be able to run initial screening analyses using data that are already available. In response to this need, we developed a long-term hydrologic impact assessment technique (L-THIA) using the popular Curve Number (CN) method that makes use of basic land use, soils and long-term rainfall data. Initially developed as a spreadsheet application, the technique allows a user to compare the hydrologic impacts of past, present and any future land use change. Consequently, a NPS pollution module was incorporated to develop the L-THWNPS model. Long-term daily rainfall records are used in combination with soils and land use information to calculate average annual runoff and NPS pollution at a watershed scale. Because of the geospatial nature of land use and soils data, and the increasingly widespread use of GE by planners, government agencies and consultants, the model is linked to a Geographic Information System (GIS) that allows convenient generation and management of model input and output data, and provides advanced visualization of the model results. Manipulation of the land use layer, or provision of multiple land use layers (for different scenarios), allows for rapid and

  15. 坡耕地不同物种植物篱对面源污染物的拦截效率及影响因素%Effects of Different Plant Species Hedgerows on Interception of Non- point Source Pollutants in Sloping Cultivated Land

    Institute of Scientific and Technical Information of China (English)

    田潇; 周运超; 蔡先立; 马礼平; 刘晓芸

    2015-01-01

    Hedgerows play important roles in control over the spread of pollutants in the environment. To investigate the effect of plant species of hedgerows on the intercepting efficiency of non-point source pollutants, a field experiment was conducted in a sloping cultivated land in the upstream of Hongfeng Lake, Guizhou. Thirty runoff plots(20 m long × 5 m width)with three banded hedgerows at equal distance were established in 15 degree sloping cultivated land. The hedgerows was planted in a criss-crossed double rows with 5 m length and 0.15 m width. Quantity of surface runoff, silt and nutrient was monitored in each runoff plot under natural raining in 2010. Results showed that there were only five surface runoffs observed after rainfall in 2010, of which two were stronger while three weaker but lasted for longer time. The greatest runoff interception was observed in the plot with Tephrosia purpurea(L.)Pers. hedgerows, with relative surface runoff intercep-tion rate of 91.75%, and the greatest silt interception was found in the plot of Medicago sativa L. hedgerows, with relative interception rate of 69.25%. Tephrosia purpurea(L.)Pers. could effectively reduce N and P loss, with interception rate about 70.08% for N(283.03 g·hm-2) and 80.21%for P(185.92 g·hm-2). However, the greatest K interception was measured in the plot with Lespedeza bicolor Turcz and fol-lowed by Tephrosia purpurea(L.)Pers. Their relative interception rates were 86.08%(135.11 g·hm-2)and 79.72%(125.13 g·hm-2), re-spectively. The present results show that hedgerows would be of great significance in controlling non-point source pollution in sloping farm-ing area.%在红枫湖上游15°的坡耕地上设置30个植物篱径流小区,小区水平投影面积100 m2,顺坡长20 m,横坡宽5 m。在小区内横坡等距种植三带植物篱,植物篱带呈双行“品”字型种植模式,带长5 m,带宽0.15 m。通过监测该年度天然降雨下每个径流小区产生的地表径流、

  16. Epidermolytic Hyperkeratosis -NPS 2 Type

    Directory of Open Access Journals (Sweden)

    Das Jayanta Kumar

    2004-01-01

    Full Text Available A case of bullous congenital ichthyosiform erythroderma (epidermolytic hyperkeratosis or EHK with some unusual features is described. It was diagnosed in a 6 month old girl with no family history of either EHK or focal lesions suggestive of mosaicism. The girl probably had EHK type NPS-2; but non-involvement of face and lack of foul body odor set the case apart.

  17. Effects of Grass Hedges and No-tillage Practice Oil the Removing of Typical Agricultural Non-point Source Pollutants from Runoff%免耕和草篱措施对径流中典型农业面源污染物的去除效果

    Institute of Scientific and Technical Information of China (English)

    李霞; 陶梅; 肖波; 王庆海; 陈建平

    2011-01-01

    Chemical fertilizer and herbicide are widely used in agricultural production. These chemical substances not only significantly increase crop yields but also result in serious water pollution. This study was conducted to evaluate the effects of three practices (no-tillage, grass hedges, no-tillage and grass hedges) on the loss of nitrogen, phosphorus and atrazine with runoff. The results showed that no-tillage and grass hedges significantly reduced agricultural non-point source pollution. As compared to the control, the total nitrogen, total phosphorus and atrazine loss in the plots with no-tillage were decreased by 53%, 51% and 56%, respectively; reductions by the grass hedges were 68%, 61% and 90%, respectively; the decreases due to no-tillage and grass hedges were 77%, 76% and 92%, respectively. Moreover, the regression results showed that the losses of nitrogen, phosphorus and atrazine with runoff were positively correlated to the surface runoff. From these results could be concluded that both of no-tillage and grass hedges could significantly reduce nitrogen, phosphorus and atrazine loss with runoff, and the integration of these two practices was proved to be much more effective than their individual effects.%大量施用农业化学物质在提高作物产量的同时,也导致了严重的水体污染.在北京地区不同坡度的径流小区上,通过人工模拟降雨试验,研究免耕、草篱、以及免耕与草篱复合3种措施对农田径流中氮、磷、阿特拉津3种典型农业面源污染物的去除效果.结果表明:免耕措施可减少53%的总氮、51%的总磷和56%的阿特拉津流失;草篱措施可减少68%的总氮、61%的总磷和90%的阿特拉津流失;而免耕与草篱复合措施可减少77%的总氮、76%的总磷和92%的阿特拉津流失.另外,总氯、总磷和阿特拉津流失量均与径流量呈显著的线性正相关关系.本研究证实,免耕和草篱措施均能有效防止氮、磷以及阿特拉津随农

  18. 紫色丘陵区典型小流域非点源磷迁移特征%Characteristics of Non-point-source Phosphorus Losses in Some Representative Land-use Sub-catchments in the Hilly Area of Purple Soil

    Institute of Scientific and Technical Information of China (English)

    沈茜; 唐家良; 朱波

    2015-01-01

    Phosphorus forms and fluxes via hydrological pathways in typical rainfall events from different land uses in the hilly area of purple soil had been conducted by in-situ monitoring in some sub-catchments so as to understand“hotspot”and “critical time”for control of non-point-source pollution of phosphorus in a catchment.Rainfall-run-off process along with sediment yield,total phosphorus (TP),dissolved phosphorus (DP)and particulate phos-phorus (PP)concentrations and loadings were monitored in representative rain (small,medium,heavy and storm) events from sub-catchment of residence,forestland and cropland.The results showed that rainfall-runoff processes responded with land uses.In the residence sub-catchment,runoff started while rainfall reached 4mm,whereas, that started in much higher rainfall (20 mm)in the forestland and cropland sub-catchment,respectively.Runoff responded to rainfall rapidly at almost the same peak in the residence sub-catchment in medium,heavy and storm rain,while runoff delayed 20 -90 and 20 -120 min after rainfall in cropland and forestland sub-catchment,re-spectively.The mean runoff depth,runoff coefficient and sediment yield from residence sub-catchment were 22.4 mm,0.36 and 136.2 kg/hm2;while those were 9.5 mm,0.09 and 48.6 kg/hm2 from forestland and 12.3mm, 0.17 and 73.5 kg/hm2 from cropland,respectively.The water and soil loss from the residence is the most serious in the hilly area of purple soil.The concentration of total P (TP)and particulate phosphorus (PP)of runoff water from the residence and forestland sub-catchment reached peak quickly and decreased sharply,whereas,TP and PP of runoff water from cropland turned into a process with multi-peaks.The concentration of dissolved P (DP)of run-off water from the residence sub-catchment decreased when runoff discharge increased.DP of runoff water from the forestland sub-catchment increased during the runoff process.DP and phosphate (PO3-4 -P)concentrations of run-off from cropland

  19. USGS-NPS Servicewide Benthic Mapping Program (SBMP) workshop report

    Science.gov (United States)

    Moses, Christopher S.; Nayagandhi, Amar; Brock, John; Beavers, Rebecca

    2010-01-01

    The National Park Service (NPS) Inventory and Monitoring (I&M) Program recently allocated funds to initiate a benthic mapping program in ocean and Great Lakes parks in alignment with the NPS Ocean Park Stewardship 2007-2008 Action Plan. Seventy-four (ocean and Great Lakes) parks, spanning more than 5,000 miles of coastline, many affected by increasing coastal storms and other natural and anthropogenic processes, make the development of a Servicewide Benthic Mapping Program (SBMP) timely. The resulting maps and associated reports will be provided to NPS managers in a consistent servicewide format to help park managers protect and manage the 3 million acres of submerged National Park System natural and cultural resources. Of the 74 ocean and Great Lakes park units, the 40 parks with submerged acreage will be the focus in the early years of the SBMP. The NPS and U.S. Geological Survey (USGS) convened a workshop (June 3-5, 2008) in Lakewood, CO. The assembly of experts from the NPS and other Federal and non-Federal agencies clarified the needs and goals of the NPS SBMP and was one of the key first steps in designing the benthic mapping program. The central needs for individual parks, park networks, and regions identified by workshop participants were maps including bathymetry, bottom type, geology, and biology. This workshop, although not an exhaustive survey of data-acquisition technologies, highlighted the more promising technologies being used, existing sources of data, and the need for partnerships to leverage resources. Workshop products include recommended classification schemes and management approaches for consistent application and products similar to other long-term NPS benthic mapping efforts. As part of the SBMP, recommendations from this workshop, including application of an improved version of the Coastal and Marine Ecological Classification Standard (CMECS), will be tested in several pilot parks. In 2008, in conjunction with the findings of this workshop

  20. NPS Focus Digital Library and Research Station

    Data.gov (United States)

    National Park Service, Department of the Interior — Scope: National. NPS Focus Digital Library and Research Station information system manages images and archives of images as well as documents described by linked...

  1. ERIC-PCR Fingerprinting of Fecal Escherichia Coli and Microbial Source Tracking in Non-point Pollution of the Shellfish Culture Area of East China Sea%大肠埃希氏菌ERIC-PCR指纹图谱构建及贝类污染微生物源示踪

    Institute of Scientific and Technical Information of China (English)

    何力; 傅玲琳; 冯立芳; 励建荣

    2012-01-01

    Objective: to establish a host-orign fecal E. Coli DNA fingerprint library by rep-PCR method using ERIC primers from livestock and poultry farms in the shellfish culture area of East China Sea. Moreover, the efficacy of microbial source tracking (MST) based on ERIC-PCR for differentiating host sources of E. Coli from shellfish and their growing waters is also evaluated. Methods: fecal samples of poultry and livestock were obtained as cloacal swabs from different farm sites in Xiangshan Bay. E. Coli isolates were purified from the fecal samples by selective cultivation and API 20E tests. The genomic DNA of E. Coli isolates was extracted by bacterial genomic DNA purification kit and fingerprinted by ERIC-PCR. InfoQuestFPTM software was used to analyze the similarities, rate of correct classification(RCC) and the stability of library. Finally, host sources of E. Coli obtained from shellfish and shellfish growing waters were predicted using MST method by Multi-Dimensional Scaling (MDS). Results: cluster analysis of ERIC-PCR DNA fingerprints of 216 E. Coli isolates revealed 37 clusters. Jack-knife analysis revealed high rate of correct classification(RCC) with 91.7%, 76.9%, 100% and 94.4% of swine, chicken, duck and goose E. Coli isolates classified into the correct host source, respectively. In addition, based on the above host-origin library by discriminant analysis, 12 unknown source strains from shellfish and growing waters were successfully discriminanted with RCC value of 78.8%. Conclusion: this work suggests that ERIC-PCR fingerprinting can be a promising genotypic tool applied in the shellfish growing water management on East Chi na Sea for source identification of fecal pollution.%目的:采用rep-PCR的ERIC引物(ERIC-PCR)构建象山港周边粪污染指示因子大肠埃希氏菌(E.coli)的DNA指纹图谱库,并根据所建DNA指纹图谱库对贝类产品污染进行微生物源示踪(Microbial Source Tracking,MST).方法:在选定的贝类养殖区域周

  2. Anthropogenic point and non-point nitrogen inputs into Huai River Basin and their impacts on riverine ammonia-nitrogen flux

    Science.gov (United States)

    Zhang, W. S.; Swaney, D. P.; Li, X. Y.; Hong, B.; Howarth, R. W.; Ding, S. H.

    2015-02-01

    This study provides a new approach to estimate both anthropogenic non-point and point nitrogen (N) inputs to the landscape, and determines their impacts on riverine ammonia-nitrogen (AN) flux, providing a foundation for further exploration of anthropogenic effects on N pollution. Our study site is Huai River Basin of China, a watershed with one of the highest levels of N input in the world. Multi-year average (2003-2010) inputs of N to the watershed are 27 200 ± 1100 kg N km-2 yr-1. Non-point sources comprised about 98% of total N input and only 2% of inputs are directly added to the aquatic ecosystem as point sources. Fertilizer application was the largest non-point source of new N to the Huai River Basin (69% of net anthropogenic N inputs), followed by atmospheric deposition (20%), N fixation in croplands (7%), and N content of imported food and feed (2%). High N inputs showed impacts on riverine AN flux: fertilizer application, point N input and atmospheric N deposition were proved as more direct sources to riverine AN flux. Modes of N delivery and losses associated with biological denitrification in rivers, water consumption, interception by dams influenced the extent of export of riverine AN flux from N sources. Our findings highlight the importance of anthropogenic N inputs from point and non-point sources in heavily polluted watersheds, and provide some implications for AN prediction and management.

  3. A case study on non-point source pollution and environmental carrying capacity of animal raising industry in subtropical watershed%亚热带流域氮磷排放与养殖业环境承载力实例研究

    Institute of Scientific and Technical Information of China (English)

    孟岑; 李裕元; 许晓光; 高茹; 王毅; 张满意; 吴金水

    2013-01-01

    畜禽养殖业粪便排放已经成为我国农村地区主要的农业面源污染源之一,也是制约养殖业良性发展的主要瓶颈.本文以湖南省长沙县典型亚热带流域为研究单元,基于流域水环境定位观测、耕地氮(N)磷(P)消纳能力以及养殖业调查和土壤分析资料,初步分析了亚热带丘陵区的面源污染现状及畜禽养殖业的环境承载力.结果表明,研究区金井河流域134.4 km2范围内N、P年负荷分别为N 2.72 t·km-2和P0.11t·km-2,其中养殖粪便对水体总氮(TN)、总磷(TP)负荷的贡献率分别约为42.2%和62.0%.区内平均畜禽养殖密度为3.46 AU·hm-2(相当于流域内年出栏生猪24.39万头),显著高于现有化肥用量条件下流域的实际承载力1.13 AU·hm-2(相当于流域内年出栏生猪6.35万头),因此养殖密度过高是导致研究区水体NP负荷较高的主要原因.区内N、P盈余量分别为N 35.8 kg·hm-2、P 18.61 kg·hm-2.研究区基本不施用化肥条件下畜禽养殖业的最大环境承载力为7.26 AU·hm-2,在有机肥占合理施肥量30%条件下,当地畜禽养殖业的环境承载力为2.74AU·hm-2(相当于流域内年出栏生猪19.50万头).降低养殖密度、调整养殖业空间布局以及提高养殖废弃物的资源化利用率是防治当前面源污染的有效途径.%The discharge of faeces from animal production is a major source of nitrogen ( N) and phosphorus (P) pollutants in the subtropical region of China. To quantify the N and F load from animal production to hydro-systems in the region, surveys on pig density and N,P inputs to farmlands, soil N and P contents, and annual observations of the N and P flows in the streams and the terminal river were carried out in a hilly watershed (134.4 km2 ) in Changsha County, Hunan Province. It was showed that the total NP loads from the watershed to the terminal river was 2.72 t and 0. 11 t·km-2·a-1, respectively ; within which 42. 2% of the N and 62. 0% of

  4. NPS Ocean Acoustics Laboratory Marine Mammal Research

    OpenAIRE

    Chiu, Ching-Sang; Collins, Curtis; Joseph, John; Margolina, Tetyana; Stimpert, Alison; Miller, Chris

    2014-01-01

    The Marine Mammal Group within the Ocean Acoustics Laboratory at NPS is involved with a range of research studying marine mammal acoustics , both sound production and effects of anthropogenic sound on marine mammals. A sampling of our research is described below.

  5. Primary care NPs: Leaders in population health.

    Science.gov (United States)

    Swartwout, Kathryn D

    2016-08-18

    A 2012 Institute of Medicine report calls primary and public healthcare workers to action, tasking them with working together to improve population health outcomes. A Practical Playbook released in 2014 enables this public health/primary care integration. Primary care NPs are in an excellent position to lead the charge and make this integration happen.

  6. Nonpoint Source Pollution.

    Science.gov (United States)

    Mccoy, Nicholas; Chao, Bing; Gang, Daniel Dianchen

    2015-10-01

    The article presents a comprehensive review of research advancing in 2014 on nonpoint source pollution (NPS). The topics presented relate to nonpoint source pollution (NPS) within agricultural and urban areas. NPS pollution from agricultural areas is the main focus in this review. Management of NPS in agricultural, urban and rural areas is presented. Modeling of NPS pollution in different watersheds with various modeling tools is reviewed.

  7. Watershed Management Tool for Selection and Spacial Allocation of Non-Point Source Pollution Control Practices

    Science.gov (United States)

    Distributed-parameter watershed models are often utilized for evaluating the effectiveness of sediment and nutrient abatement strategies through the traditional {calibrate→ validate→ predict} approach. The applicability of the method is limited due to modeling approximations. In ...

  8. Palladium-phosphorus/sulfur nanoparticles (NPs) decorated on graphene oxide: synthesis using the same precursor for NPs and catalytic applications in Suzuki-Miyaura coupling

    Science.gov (United States)

    Joshi, Hemant; Sharma, Kamal Nayan; Sharma, Alpesh K.; Singh, Ajai Kumar

    2014-04-01

    PdP2 and Pd4S nanoparticles (NPs) (size: ~2-6 and 9-15 nm respectively) have been prepared for the first time from a single source precursor complex [Pd(L)Cl2] (1) by its one pot thermolysis at 200 °C in TOP and OA/ODE (1 : 1) respectively. These NPs were stirred with graphene oxide (GO) at room temperature to prepare NP composites, GO-PdP2 and GO-Pd4S. The GO-PdP2 NPs have been synthesized for the first time. The thioether ligand L prepared by reaction of 1,3-dibromo-2-propanol with the in situ generated PhSNa reacts with [PdCl2(CH3CN)2] in CH3CN at 70 °C resulting in 1. The L and 1 have been characterized by 1H and 13C{1H} NMR and HR-MS. The single crystal structure of 1 determined by X-ray diffraction reveals nearly square planar geometry around the Pd metal centre. The catalytic activities of two palladium nano-phases having phosphorus and sulphur respectively as a co-constituent for Suzuki-Miyaura coupling have been found to be exceptionally different, as PdP2 nanoparticles (NPs) grafted on graphene oxide (GO-PdP2) are significantly more efficient than Pd4S NPs grafted on GO. Without grafting PdP2 and Pd4S both have low efficiency. This is the first report comparing the influence of P and S on the catalytic activity of Pd NPs. TEM, SEM-EDX and powder-XRD have been used to authenticate all NPs. The GO-PdP2 NPs have been found to be efficient catalysts for Suzuki-Miyaura coupling reactions (yield up to 96% in 30 min) at room temperature to 80 °C. Their recyclability has been found up to 6 cycles. In contrast, GO-Pd4S NPs are little active in comparison with GO-PdP2 NPs. The size of NPs and their distribution on GO appear to be key factors affecting the catalytic efficiency of the composite NPs. Leaching of Pd from GO-PdP2 NPs contributes significantly to the catalysis as evidenced by the three phase test, hot-filtration and recycling experiments. The catalysis is almost homogeneous.PdP2 and Pd4S nanoparticles (NPs) (size: ~2-6 and 9-15 nm respectively) have

  9. Application of Potential Non-Point Pollution Index For An Urban Watershed: Istanbul, Kucukcekmece Lagoon

    Science.gov (United States)

    Musaoglu, N.; Dikerler, T.; Seker, D. Z.; Ustun, B.

    2011-12-01

    Istanbul is a major city with more than 15 million population and limited water resources. Besides, its urbanized area has been rapidly expanding for more than 30 years. Küçükçekmece Lagoon, as a potential RAMSAR site with its rich natural diversity and housing asset for birds, has been suffering from urbanization and industrial stress. With Sazlidere Dam constructed on the Lagoon's most important creek which supplies fresh water, Küçükçekmece Basin has almost 600 km2 wide area. Due to dam operation which cuts fresh water input down, water quality of the Küçükçekmece Lagoon has been deteriorating, as well as other antropogenic impacts. Potential non-point pollution index (or PNPI) is based on land use, soil and topographic data and aims to highlight the potentially polluting areas in a watershed. Denoting those areas, PNPI puts an assessment of the pressure exerted on the water bodies by different land uses. This index calculates different layers in order to represent run-off, land cover effect, and the distance of each polluting source (or pixels) in the study area. By the multiplication of those layers under GIS, a new data layer is produced showing the polluting potential of each pixel on the study area. For by Küçükçekmece Basin, Landsat ETM satellite images have been taken and its land use produced by unsupervised classification. Using this updated data, land use - land cover indicator has been calculated for the basin. Topography is another fact that is needed to produce both run-off indicator and distance indicator and it is generated by elevation data with 5m resolution. By integrating these indicator layers, PNPI analysis layer has been produced for Küçükçekmece Lagoon watershed.

  10. Piezoelectric electrospun nanocomposite comprising Au NPs/PVDF for nerve tissue engineering.

    Science.gov (United States)

    Motamedi, Asma Sadat; Mirzadeh, Hamid; Hajiesmaeilbaigi, Fereshteh; Bagheri Khoulenjani, Shadab; Shokrgozar, Mohammad Ali

    2017-03-03

    In this study, gold nanoparticles/Polyvinylidenefluoride composite electrospun mat with enhanced piezoelectricity were fabricated and characterized. Gold colloidal nanoparticles (Au NPs) were prepared via laser ablation of metallic targets in liquid media. The active Q-switched Nd:YAG laser was used as an irradiation source. Then, PVDF (Polyvinylidenefluoride) was dissolved in Au NPs colloidal solution at 30% wt for the synthesis of Au NPs/PVDF composite nanofibers by electrospinning. The optical absorbance spectra of Au NPS and the polymeric solutions were obtained by the UV-Visible spectroscopy. Moreover, the morphology of Au NPS, nanostructures of fibers and diameter size distribution of nanofibers were analyzed by Scanning Electron Microscopy (SEM), Field Emission Scanning Electron Microscopy (FESEM) and Transmitted Electron Microscopy (TEM) methods. The crystallinity and piezoelectricity of PVDF and Au NPs/PVDF composite nanofibers mats were measured by X-Ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) methods. Subsequently, in vitro cytocompatibility was evaluated by MTT assay and the attachment and morphology of PC-12 cells cultured on scaffolds were studied. It was found that laser ablated Au NPs can be used in electrospun nanofibers of PVDF with adequate structural properties and increase piezoelectricity of nanofibers which might be suitable for applying as nerve tissue engineering scaffolds. This article is protected by copyright. All rights reserved.

  11. Bio-Economic Strategy to Combat Non-Point Pollution in China

    Institute of Scientific and Technical Information of China (English)

    WEI Yong-Ping; CHEN De-Li; B.DAVIDSON; R.E.WHITE

    2005-01-01

    While non-point pollution from agriculture has become an increasingly serious problem in China, some progress has been made in studying the causal biophysical processes. However, few studies have assessed the economic consequences of non-point pollution in China or the policy options that could be employed to combat it. In this work a sustainable strategy to control non-point pollution from crop production, which involved taxing excessive inputs of irrigation water and fertilizer, was proposed. The approach taken to assess these measures combined biophysical and economic models,having a trade-off between economic returns and an improved environment. A proven and practical spatially referenced water and nutrient management model was used to determine the quantities of excessive irrigation water and fertilizer for specific soil and land use. Also, a set of indicators were proposed for evaluating the effects of agricultural economic output and agricultural practices on the environment.

  12. Individual and Co Transport Study of Titanium Dioxide NPs and Zinc Oxide NPs in Porous Media.

    Directory of Open Access Journals (Sweden)

    Jyoti Kumari

    Full Text Available The impact of pH and ionic strength on the mobility (individual and co-transport and deposition kinetics of TiO2 and ZnO NPs in porous media was systematically investigated in this study. Packed column experiments were performed over a series of environmentally relevant ionic strengths with both NaCl (0.1-10 mM and CaCl2 (0.01-0.1mM solutions and at pH 5, 7, and 9. The transport of TiO2 NPs at pH 5 was not significantly affected by ZnO NPs in solution. At pH 7, a decrease in TiO2 NP transport was noted with co-existence of ZnO NPs, while at pH 9 an increase in the transport was observed. At pH 5 and 7, the transport of ZnO NPs was decreased when TiO2 NPs was present in the solution, and at pH 9, an increase was noted. The breakthrough curves (BTC were noted to be sensitive to the solution chemistries; the decrease in the breakthrough plateau with increasing ionic strength was observed under all examined pH (5, 7, and 9. The retention profiles were the inverse of the plateaus of BTCs, as expected from mass balance considerations. Overall, the results from this study suggest that solution chemistries (ionic strength and pH are likely the key factors that govern the individual and co-transport behavior of TiO2 and ZnO NPs in sand.

  13. Can non-point pollutions emissions from agriculture be regulated efficiently using input-output taxes?

    DEFF Research Database (Denmark)

    Hansen, Line Block; Hansen, Lars Gårn

    2012-01-01

    In many parts of Europe and North America, phosphorus loss from cultivated fields is threatening natural ecosystems. Though there are similarities to other non-point agricultural emissions like nitrogen that have been studied extensively, phosphorus is often characterized by the presence of large...

  14. Eutrophication assessment and management methodology of multiple pollution sources of a landscape lake in North China.

    Science.gov (United States)

    Chen, Yanxi; Niu, Zhiguang; Zhang, Hongwei

    2013-06-01

    Landscape lakes in the city suffer high eutrophication risk because of their special characters and functions in the water circulation system. Using a landscape lake HMLA located in Tianjin City, North China, with a mixture of point source (PS) pollution and non-point source (NPS) pollution, we explored the methodology of Fluent and AQUATOX to simulate and predict the state of HMLA, and trophic index was used to assess the eutrophication state. Then, we use water compensation optimization and three scenarios to determine the optimal management methodology. Three scenarios include ecological restoration scenario, best management practices (BMPs) scenario, and a scenario combining both. Our results suggest that the maintenance of a healthy ecosystem with ecoremediation is necessary and the BMPs have a far-reaching effect on water reusing and NPS pollution control. This study has implications for eutrophication control and management under development for urbanization in China.

  15. Can non-point pollutions emissions from agriculture be regulated efficiently using input-output taxes?

    DEFF Research Database (Denmark)

    Hansen, Line Block; Gårn Hansen, Lars

    2014-01-01

    In many parts of Europe and North America, phosphorus loss from cultivated fields is threatening natural ecosystems. Though there are similarities to other non-point agricultural emissions like nitrogen that have been studied extensively, phosphorus is often characterized by the presence of large...... systems. Depending on the proportions of different types of farms in the agricultural sector, we find that an input-output tax system can be the core element of a close to efficient regulatory policy....

  16. SIMULATION OF NON-POINT POLLUTANTS EVOLUTION IN COASTAL PLAIN ISLAND-A CASE STUDY OF CHONGMING ISLAND

    Institute of Scientific and Technical Information of China (English)

    YIN Hai-long; JIANG Wen-yan; LI Jian-hua

    2008-01-01

    The coastal plain region usually shows the agricultural dominated industry mode, so more and more attention is paid to non-point pollutants discharge. In this study, the method for assessing the influence of non-point pollutants discharge on river water quality is probed which is concluded as follows: (1) Considering the costal plain island is characterized as nearly even elevation, the conventional gravitational runoff confluence method based on eight-point gravitational flowing directions (D8) is unable to compute the runoff confluence received by the surrounding rivers, so a new method of triangular confluence based on three-points of grid center and the river segments is presented. Further the equations of non-point pollutants loads estimation and non-point pollutants confluence on rivers are presented. (2) The integration mode of non-point pollutants model and river water quality model is presented so as to further assess the non-point pollutants contribution to river water quality. (3) With the Chongming Island, the third largest island in China, as an example, the above-mentioned equations are specified. For this island, the non-point pollutants loads are estimated and linked to the developed water quality model of the river network in the island, and further the non-point pollution evolution in river network is simulated. In this scenario, the non-point pollution contribution to river water quality over the whole island is clearly displayed, and the area where the river water quality is seriously influenced by non-point pollutants discharge is distinctly depicted. This scenario also shows the water quality contribution ratio of non-point pollution to point pollution can be in the range of 55.5% to 44.5% which proves the importance of non-point pollution control in costal plain islands.

  17. [Non-point loads of soluble cadmium by in situ field experiment with different landuses, in central Hunan province mining area].

    Science.gov (United States)

    Liu, Xiao-li; Zeng, Zhao-xia; Chen, Zhe; Tie, Bai-qing; Chen, Qiu-wen; Ye, Chang-cheng

    2013-09-01

    Non-point source loads of heavy metals from contaminated soil has increasingly become the major cause of heavy metal concentrations of rivers and lakes surpassed the limitation value, while only few studies had focused on quantitative monitoring of soil heavy metal transportation to water, in situ field conditions. As reported, agricultural farmland heavy metal contamination was the major contamination problem, especially for cadmium (Cd) pollution in middle and downstream of Xiangjiang River. This study selected the typical Cd polluted agricultural watershed for a case study, three typical landuse types of rice, dry farmland and unused grassland with three replicate quadrates were carried out for natural rainfall runoff hydrology processes monitoring, from 2011-2012. Results showed that, precipitation pH value increased from spring to summer, soluble Cd concentration of spring runoff was significantly higher than that of summer rainfall runoff, which presented an obviously seasonal heterogeneity and had a negative correlation with rainfall pH value, and rainfall pH value can obviously impact soil soluble Cd transportation into surface runoff charge. In the same rainfall event, soluble Cd concentration and non-point load of rice were significantly lower than those of dry land and unused grassland, while no obviously seasonal trend was found for non-point load of Cd from three typical landuse types because of the rainfall depth variance, which needs more researches and concerns in the future. These results can provide valuable data and scientific supports for watershed scale's heavy metal non-point source load quantitative estimation and water environment management and water quality diagnosis and early warning.

  18. Can non-point pollutions emissions from agriculture be regulated efficiently using input-output taxes?

    DEFF Research Database (Denmark)

    Hansen, Line Block; Hansen, Lars Gårn

    2012-01-01

    In many parts of Europe and North America, phosphorus loss from cultivated fields is threatening natural ecosystems. Though there are similarities to other non-point agricultural emissions like nitrogen that have been studied extensively, phosphorus is often characterized by the presence of large...... systems. Depending on the proportions of different types of farms in the agricultural sector, we find that an input-output tax system may be close to efficient, or in other cases must be supplemented with subsidy and manure reallocation schemes....

  19. Synthesis of Green Metallic Nanoparticles (NPs and Applications

    Directory of Open Access Journals (Sweden)

    Varahalarao Vadlapudi

    2014-01-01

    Full Text Available Nanotechnology (NT is a field that is mushrooming, making an impact in all spheres of human life. Presently available literature revealed that the NP synthesis using medicinal plants, microorganisms and algae and others as source has been unexplored and underexploited. NT is very important in developing sustainable technologies for the future, for humanity and the environment. The development of green processes for the synthesis of NP is evolving into an important branch of nanotechnology. Natural sources of nanoparticles include fires and volcanic eruptions. The nanotechnology have short to long term uses like environmental pollution cleanup, efficient and safe drug delivery mechanisms with less side effects, developments in information technology, self-cleaning window glass , ‘smart’ fabrics which adjust to suit the temperature. Plant mediated synthesis of metal nanoparticles is gaining more importance owing to its simplicity, rapid rate of synthesis of NP of attractive and diverse morphologies and elimination of elaborate maintenance of cell cultures and ecofriendliness. It has many advantages such as, ease with which the process can be measured up, economic viability and etc. Presently, the researchers are looking into the development of cost-effective procedures for producing reproducible, stable and biocompatible metal NPs.

  20. One pot biosynthesis of gold NPs using red cabbage extracts.

    Science.gov (United States)

    Lekeufack, Diane D; Brioude, Arnaud

    2012-02-07

    Red cabbage extract was used as reducing agent and capping agent for the synthesis of gold NPs. The method developed is environmentally friendly and allows the control of NPs shape and size by changing the pH value and the concentration of aqueous red cabbage extract solution.

  1. Can non-point phosphorus emissions from agriculture be regulated efficiently using input-output taxes?

    DEFF Research Database (Denmark)

    Hansen, Line Bloch; Hansen, Lars Gårn

    In many parts of Europe and North America, phosphorus loss from cultivated fields is threatening natural ecosystems. Though there are similarities to other non-point agricultural emissions like nitrogen that have been studied extensively, phosphorus is often characterised by the presence of large...... stocking capacities for phosphorus in farm soils and long time-lags between applications and emission. This makes it important to understand the dynamics of the phosphorus emission problem when designing regulatory systems. Using a model that reflects these dynamics, we evaluate alternative regulatory...... systems. Depending on the proportions of different types of farms in the agricultural sector, we find that an input-output tax system may be close to efficient, or in other cases must be supplemented with subsidy and manure reallocation schemes....

  2. Allosteric activation of the Ca2+ receptor expressed in Xenopus laevis oocytes by NPS 467 or NPS 568.

    Science.gov (United States)

    Hammerland, L G; Garrett, J E; Hung, B C; Levinthal, C; Nemeth, E F

    1998-06-01

    The Ca2+ receptor is a G protein-coupled receptor that enables parathyroid cells and certain other cells in the body to respond to changes in the concentration of extracellular Ca2+. In this study, two novel phenylalkylamine compounds, NPS 467 and NPS 568, were examined for effects on Xenopus laevis oocytes expressing the bovine or human parathyroid Ca2+ receptors. Increases in chloride current (ICl) were elicited in oocytes expressing the bovine Ca2+ receptor when the extracellular Ca2+ concentration was raised above 1.5 mM, whereas Ca2+ concentrations > 3 mM were generally necessary to elicit responses in oocytes expressing the human Ca2+ receptor. NPS 467 and NPS 568 potentiated the activation of ICl by extracellular Ca2+ in oocytes expressing either Ca2+ receptor homolog, and this resulted in a leftward shift of the Ca2+ concentration-response curve. Neither compound was active in the absence of extracellular Ca2+. Certain inorganic and organic cations known to activate the Ca2+ receptor were substituted for elevated levels of extracellular Ca2+ to increase ICl and the effects of these agonists were also potentiated by NPS 568 or NPS 467. The effects of NPS 568 were stereoselective and the R-enantiomer was about 10-fold more potent than the corresponding S-enantiomer. Neither NPS 467 nor 568 affected ICl in water-injected oocytes or in oocytes expressing the substance K receptor or the metabotropic glutamate receptor 1a. These results provide compelling evidence that NPS 467 and NPS 568 act directly upon the parathyroid Ca2+ receptor to increase its sensitivity to activation by extracellular Ca2+. This activity suggests that these compounds are positive allosteric modulators of the Ca2+ receptor. As such, these compounds define a new class of pharmacological agents with potent and selective actions on the Ca2+ receptor.

  3. Polluted dust derived from long-range transport as a major end member of urban aerosols and its implication of non-point pollution in northern China.

    Science.gov (United States)

    Yan, Y; Sun, Y B; Weiss, D; Liang, L J; Chen, H Y

    2015-02-15

    The contribution of polluted dust transported from local and distal sources remains poorly constrained due to their similar geophysical and geochemical properties. We sampled aerosols in three cities in northern China (Xi'an, Beijing, Xifeng) during the spring of 2009 to determine dust flux, magnetic susceptibility and elemental concentrations. Combining dust fluxes with wind speed and regional visibility records enabled to differentiate between dust transported from long range and derived from local sources, while the combination of magnetic susceptibility and enrichment factors (EF) of heavy metals (Pb, Zn) allowed to distinguish natural aerosols from polluted ones. Our results indicate that polluted dust from long-range transport became a major end member of urban dust aerosols. Human settlements as its potential sources were confirmed by a pollutant enriched regional dust event originating from populated areas to the south as inferred by back trajectory modeling, implying their non-point source nature of dust pollution.

  4. NPS Patent Office Helps Researchers Safeguard Intellectual Property

    OpenAIRE

    Stewart, Kenneth A.

    2014-01-01

    Associate Council, Intellectual Property Attorney Lisa Norris, and her assistant, Paralegal Kate Black-Will, are an Army of two, with a singular mission to protect the intellectual property of NPS students and faculty.

  5. NPS Defense Management Expert Elected to ASPA National Council

    OpenAIRE

    Honegger, Barbara

    2007-01-01

    Naval Postgraduate School (NPS) George F. A. Wagner Professor of Public Management Lawrence R. "Larry" Jnoes, a world renowned expert on defense budgeting and financial management, has been elected to National Council of the American Society for Public Administration (ASPA),

  6. Transport of silver nanoparticles (AgNPs) in soil.

    Science.gov (United States)

    Sagee, Omer; Dror, Ishai; Berkowitz, Brian

    2012-07-01

    The effect of soil properties on the transport of silver nanoparticles (AgNPs) was studied in a set of laboratory column experiments, using different combinations of size fractions of a Mediterranean sandy clay soil. The AgNPs with average size of ~30nm yielded a stable suspension in water with zeta potential of -39mV. Early breakthrough of AgNPs in soil was observed in column transport experiments. AgNPs were found to have high mobility in soil with outlet relative concentrations ranging from 30% to 70%, depending on experimental conditions. AgNP mobility through the column decreased when the fraction of smaller soil aggregates was larger. The early breakthrough pattern was not observed for AgNPs in pure quartz columns nor for bromide tracer in soil columns, suggesting that early breakthrough is related to the nature of AgNP transport in natural soils. Micro-CT and image analysis used to investigate structural features of the soil, suggest that soil aggregate size strongly affects AgNP transport in natural soil. The retention of AgNPs in the soil column was reduced when humic acid was added to the leaching solution, while a lower flow rate (Darcy velocity of 0.17cm/min versus 0.66cm/min) resulted in higher retention of AgNPs in the soil. When soil residual chloride was exchanged by nitrate prior to column experiments, significantly improved mobility of AgNPs was observed in the soil column. These findings point to the importance of AgNP-soil chemical interactions as a retention mechanism, and demonstrate the need to employ natural soils rather than glass beads or quartz in representative experimental investigations.

  7. Behind the scenes w/ an NPS alum (poster)

    OpenAIRE

    Reber, Ethan

    2012-01-01

    How NPS helped my Navy M&S career: "The Naval Postgraduate School provided the technical, analytical, research and critical thinking skills I required to excel as the Navigation, Seamanship, and Shiphandling Simulation Program Manage at the U.S. Navy's Shiphandling Center of Excellence, the Surface Warfare Officers School. No prgogram other than NPS' Modeling, Virtual Environments and Simulation (MOVES) curriculum provides such a full range of topics, skills, and joint service institutional ...

  8. Photoluminescent colloidal Cu@C-NPs suspensions synthesized by LASL

    Science.gov (United States)

    Reyes-Contreras, D.; González-Aguilar, M. A.; Camacho-López, M. A.; Vigueras-Santiago, E.; Camacho-López, M.

    2017-05-01

    In this work we report the synthesis of photoluminescent carbon-coated copper nanoparticles (Cu@C-NPs) based colloidal suspensions, using the laser ablation of solids in liquids technique (LASL). LASL experiments were carried out by ablating a Cu solid target immersed in acetone as the liquid medium with ns-laser pulses (1064 nm) of a Nd-YAG laser. In all experiments the per pulse laser fluence and the repetition rate frequency were kept constant and the ablation time was varied. The as obtained Cu@C-NPs suspensions were optically characterized with absorption and photoluminescence spectroscopies. Raman spectroscopy was used to give evidence of the carbon shell deposited around the Cu NPs. TEM results showed that 10 nm spheroids Cu@C-NPs were obtained. The as obtained Cu@C-NPs suspensions displayed out a PL emission band similar to that for carbon nanoparticles suspensions obtained by the same technique. We have found that the blue-green PL emission band intensity is mainly dependent on the ablation and aging time of the samples. The Cu@C-NPs-based colloidal suspensions can be proposed as multifunctional due to its absorbance and emission properties.

  9. Novel Psychoactive Substances (NPS: a Study on Persian Language Websites

    Directory of Open Access Journals (Sweden)

    Fabrizio Schi¬Fano

    2013-05-01

    Full Text Available Background: During the past few years, there has been an increasing recognition that Internet is playing a significant role in the synthesis, the distribution and the consumption of Novel Psychoactive Substances (NPS.The aim of this study was to assess the online availability of NPS in Persian language websites.Methods: The Google search engine was used to carry out an accurate qualitative assessment of information available on NPS in a sample of 104 websites.Results: The monitoring has led to the identification of 14 NPS including herbal, synthetic, pharmaceutical and combination drugs that have been sold online.Conclusion: The availability of online marketing of NPS in Persian language websites may constitute a public health challenge at least across three Farsi-speaking countries in the Middle East. Hence, descriptions of this phenomenon are valuable to clinicians and health professional in this region. Further international collaborative efforts may be able to tackle the growth and expansion of regular offer of NPS.

  10. Event-based nonpoint source pollution prediction in a scarce data catchment

    Science.gov (United States)

    Chen, Lei; Sun, Cheng; Wang, Guobo; Xie, Hui; Shen, Zhenyao

    2017-09-01

    Quantifying the rainfall-runoff-pollutant (R-R-P) process is key to regulating non-point source (NPS) pollution; however, the impacts of scarce measured data on R-R-P simulations have not yet been reported. In this study, we conducted a comprehensive study of scarce data that addressed both rainfall-runoff and runoff-pollutant processes, whereby the impacts of data scarcity on two commonly used methods, including Unit Hydrograph (UH) and Loads Estimator (LOADEST), were quantified. A case study was performed in a typical small catchment of the Three Gorges Reservoir Region (TGRR) of China. Based on our results, the classification of rainfall patterns should be carried out first when analyzing modeling results. Compared to data based on a missing rate and a missing location, key information generates more impacts on the simulated flow and NPS loads. When the scarcity rate exceeds a certain threshold (20% in this study), measured data scarcity level has clear impacts on the model's accuracy. As the model of total nitrogen (TN) always performs better under different data scarcity conditions, researchers are encouraged to pay more attention to continuous the monitoring of total phosphorus (TP) for better NPS-TP predictions. The results of this study serve as baseline information for hydrologic forecasting and for the further control of NPS pollutants.

  11. Continuous 'Passive' Registration of Non-Point Contaminant Loads Via Agricultural Subsurface Drain Tubes

    Science.gov (United States)

    Rozemeijer, J.; Jansen, S.; de Jonge, H.; Lindblad Vendelboe, A.

    2014-12-01

    Considering their crucial role in water and solute transport, enhanced monitoring and modeling of agricultural subsurface tube drain systems is important for adequate water quality management. For example, previous work in lowland agricultural catchments has shown that subsurface tube drain effluent contributed up to 80% of the annual discharge and 90-92% of the annual NO3 loads from agricultural fields towards the surface water. However, existing monitoring techniques for flow and contaminant loads from tube drains are expensive and labor-intensive. Therefore, despite the unambiguous relevance of this transport route, tube drain monitoring data are scarce. The presented study aimed developing a cheap, simple, and robust method to monitor loads from tube drains. We are now ready to introduce the Flowcap that can be attached to the outlet of tube drains and is capable of registering total flow, contaminant loads, and flow-averaged concentrations. The Flowcap builds on the existing SorbiCells, a modern passive sampling technique that measures average concentrations over longer periods of time (days to months) for various substances. By mounting SorbiCells in our Flowcap, a flow-proportional part of the drain effluent is sampled from the main stream. Laboratory testing yielded good linear relations (R-squared of 0.98) between drainage flow rates and sampling rates. The Flowcap was tested in practice for measuring NO3 loads from two agricultural fields and one glasshouse in the Netherlands. The Flowcap registers contaminant loads from tube drains without any need for housing, electricity, or maintenance. This enables large-scale monitoring of non-point contaminant loads via tube drains, which would facilitate the improvement of contaminant transport models and would yield valuable information for the selection and evaluation of mitigation options to improve water quality.

  12. Assessment of Network and Data Communication Reliability for Lungmen NPS

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, Teng Chieh; Chou, Hwai Pwu [National Tsing Hua University, Hsinchu (China); Chao, Chun Chang [The Institute of Nuclear Energy Research, Taoyuan (China)

    2011-08-15

    The Lungmen nuclear power station (NPS) is an advanced boiling water reactor (ABWR) with fully digitized instrumentation and control (I and C) system. The present work is to use the probabilistic risk assessment (PRA) technique to investigate the concerns about network reliability and data communication errors for Lungmen NPS. The reactor protection system (RPS) has chosen as the target to investigate the network and data communication reliability. A fault tree based on the RPS configuration has been modeled to evaluate the weak point in the digital logic part. A Lungmen NPS event tree model has also built to calculate the core damage frequency (CDF). Sensitivity studies were performed by assuming various data communication delays and errors and to evaluate the network affect and the influence on the CDF.

  13. A repeated-measures study of recreational water exposure, non-point source pollution, and risk of illness

    Science.gov (United States)

    Discharge of stormwater runoff onto beaches is a major cause of beach closings and advisories in the United States. Prospective studies of recreational water quality and health have often been limited to two time points (baseline and follow-up). Little is known about the risk of ...

  14. Seasonal change of non-point source pollution-induced bioavailable phosphorus loss: A case study of Southwestern China

    Science.gov (United States)

    Gao, Yang; Zhu, Bo; Wang, Tao; Wang, Yafeng

    2012-02-01

    SummaryBioavailable phosphorus (P) losses due to agriculture activity in a purple soil watershed in the Sichuan Basin of Southwestern China were monitored to define the hydrological controls of P transport. Our results indicate that the proportion of P that was transported in particulate form increased in the rainy season, and that the mass of total bioavailable P (BAP) loads exhibited seasonal fluctuations, wherein the majority (over 90%) was observed to have been exported between June and September. The proportion of bioavailable dissolved P (BDP) in the BAP discharge budget in the watershed varied between 11% and 15% during the monitoring period. The bioavailable particulate P (BPP) and BDP concentrations of stream water under rainstorm events increased by over 40% in comparison to their annual mean concentrations, and the annual BAP load was primarily dominated by the loads that occurred during rainstorm events in the study year. BAP concentration in groundwater significantly fluctuated with the seasons, and the ratio of total BAP in groundwater to that in surface water gradually increased during the rainy season. Thus, the impact of agriculture on the water quality of this watershed becomes clearly evident.

  15. WA - Impacts of non-point source pollution on the health of salmonids in urban and urbanizing watersheds

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — By the Services conducting this off-refuge EC investigation and jointly publishing the results in the two aforementioned manuscripts [see notes section of ProCite...

  16. Modeling hydrology, groundwater recharge and non-point nitrate loadings in the Himalayan Upper Yamuna basin.

    Science.gov (United States)

    Narula, Kapil K; Gosain, A K

    2013-12-01

    The mountainous Himalayan watersheds are important hydrologic systems responsible for much of the water supply in the Indian sub-continent. These watersheds are increasingly facing anthropogenic and climate-related pressures that impact spatial and temporal distribution of water availability. This study evaluates temporal and spatial distribution of water availability including groundwater recharge and quality (non-point nitrate loadings) for a Himalayan watershed, namely, the Upper Yamuna watershed (part of the Ganga River basin). The watershed has an area of 11,600 km(2) with elevation ranging from 6300 to 600 m above mean sea level. Soil and Water Assessment Tool (SWAT), a physically-based, time-continuous model, has been used to simulate the land phase of the hydrological cycle, to obtain streamflows, groundwater recharge, and nitrate (NO3) load distributions in various components of runoff. The hydrological SWAT model is integrated with the MODular finite difference groundwater FLOW model (MODFLOW), and Modular 3-Dimensional Multi-Species Transport model (MT3DMS), to obtain groundwater flow and NO3 transport. Validation of various modules of this integrated model has been done for sub-basins of the Upper Yamuna watershed. Results on surface runoff and groundwater levels obtained as outputs from simulation show a good comparison with the observed streamflows and groundwater levels (Nash-Sutcliffe and R(2) correlations greater than +0.7). Nitrate loading obtained after nitrification, denitrification, and NO3 removal from unsaturated and shallow aquifer zones is combined with groundwater recharge. Results for nitrate modeling in groundwater aquifers are compared with observed NO3 concentration and are found to be in good agreement. The study further evaluates the sensitivity of water availability to climate change. Simulations have been made with the weather inputs of climate change scenarios of A2, B2, and A1B for end of the century. Water yield estimates under

  17. Effects of Forest Ecosystems on Non-point Pollution Control%森林生态系统对非点源污染的控制机理与效果及其影响因素

    Institute of Scientific and Technical Information of China (English)

    张灿强; 张彪; 李文华; 杨艳刚; 王斌

    2011-01-01

    Non-point source pollution, particularly agricultural non-point source pollution, has become a major contributor of water pollution in many countries during recent years. Major lakes and rivers in China have suffered from serious eutrophication problems, e.g., five lakes (Poyang Lake, Dongting Lake, Taihu Lake, Hongze Lake, and Chaohu Lake), the Three Gorges Reservoir,Baiyangdian Lake, Nansi Lake, and Yilong Lake. The contribution rate of non-point pollution to eutrophication has been found to be much higher than point source pollution. Although tremendous efforts have been made to control non-point pollution, they seem to be far from effective due to uncertain emissions of the non-point pollutants. As such, people are resorting to purifying natural ecosystems, e.g., forest riparian buffer has been recommended as one of the Best Management Practices (BMP) for non-point source pollutant control in the United States. The authors comprehensively summarized studies associated with the effects of forest ecosystems on non-point source pollution control. Forest ecosystems have been shown to be of the capacity to adsorb and purify non-point source pollutants mainly through plants, canopy, ground litter, and soil layers.Plant uptake is one of important mechanisms of nutrients control. Part of pollutions carried by rainfall can be intercepted by canopy, while the nutrient content will increase because of eluviations. However, the non-point pollution can be greatly reduced after runoff flow through ground cover and soil layer. Numerous studies show that forests could reduce about 60~90% of the total nitrogen and phosphorus from upper farming areas. Non-point source pollution control is generally affected by many factors. The forest belt width has an impact on the effect of non-point source pollution reduction, and therefore the width needs to be adjusted appropriately. Different tree species show varying absorption capacities of non-point source pollutants; therefore the

  18. A Study on the Mechanism of Emission Trading between Industrial Point Pollution and Agriculture Non-Point Pollution%工业点源-农业面源排污权交易的机制创新研究

    Institute of Scientific and Technical Information of China (English)

    王奇; 王会; 陈海丹; 詹贤达

    2011-01-01

    Agriculture non-point pollution has been one of the most important sources of the water pollution in China.Compared with industrial point pollution, the emission reduction cost of agriculture non-point pollution is lower, which make the emission trade feasible. First, the characteristics of agriculture non-point pollution were recognized as that agriculture non-point pollution had many sources, uncertainty in occurring, difficulty to supervise. Then, two emission trade mechanisms had been built, one of which was that farmers changed production behavior uniformly and the other was that industrial point pollution built public pollution eliminating infrastructures. Finally, some key problem in the emission trading between industrial point pollution and agriculture non-point pollution were discussed.%当前,农业面源污染已经成为我国水环境污染的主要来源.相比较于大型工业点源,农业面源污染减排成本较低的状况为二者进行排污权交易提供了有利条件.在分析面源污染具有涉及个体多、发生不确定性强、减排结果监测困难等特征的基础上,提出了工业点源与农业面源排污权交易的两种机制:基于污染产生的"农户统一行动交易机制"和基于污染治理的"农业公共治理设施建设交易机制".最后,针对工业点源-农业面源排污权交易中存在的一些问题进行了初步探讨.

  19. Modeling hydrology, groundwater recharge and non-point nitrate loadings in the Himalayan Upper Yamuna basin

    Energy Technology Data Exchange (ETDEWEB)

    Narula, Kapil K., E-mail: kkn2104@columbia.edu [Columbia Water Center (India Office), Columbia University, New Delhi 110 016 (India); Gosain, A.K. [Department of Civil Engineering, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110 016 (India)

    2013-12-01

    The mountainous Himalayan watersheds are important hydrologic systems responsible for much of the water supply in the Indian sub-continent. These watersheds are increasingly facing anthropogenic and climate-related pressures that impact spatial and temporal distribution of water availability. This study evaluates temporal and spatial distribution of water availability including groundwater recharge and quality (non-point nitrate loadings) for a Himalayan watershed, namely, the Upper Yamuna watershed (part of the Ganga River basin). The watershed has an area of 11 600 km{sup 2} with elevation ranging from 6300 to 600 m above mean sea level. Soil and Water Assessment Tool (SWAT), a physically-based, time-continuous model, has been used to simulate the land phase of the hydrological cycle, to obtain streamflows, groundwater recharge, and nitrate (NO{sub 3}) load distributions in various components of runoff. The hydrological SWAT model is integrated with the MODular finite difference groundwater FLOW model (MODFLOW), and Modular 3-Dimensional Multi-Species Transport model (MT3DMS), to obtain groundwater flow and NO{sub 3} transport. Validation of various modules of this integrated model has been done for sub-basins of the Upper Yamuna watershed. Results on surface runoff and groundwater levels obtained as outputs from simulation show a good comparison with the observed streamflows and groundwater levels (Nash–Sutcliffe and R{sup 2} correlations greater than + 0.7). Nitrate loading obtained after nitrification, denitrification, and NO{sub 3} removal from unsaturated and shallow aquifer zones is combined with groundwater recharge. Results for nitrate modeling in groundwater aquifers are compared with observed NO{sub 3} concentration and are found to be in good agreement. The study further evaluates the sensitivity of water availability to climate change. Simulations have been made with the weather inputs of climate change scenarios of A2, B2, and A1B for end of the

  20. Regulation og non-point phosphorus emissions from the agricultural sector by use of economic incentives

    DEFF Research Database (Denmark)

    Hansen, Line Block

    . Once a soil is highly enriched it will become a significant source of P losses for a long time. In Denmark, application of manure is primarily determined by nitrogen (N) crop nutrition, implying that livestock farmers are not motivated to further reduce their surplus P applications. The high costs...

  1. Controlled-release fertilizer(CRF):A green fertilizer for controlling non-point contamination in agriculture

    Institute of Scientific and Technical Information of China (English)

    MAO Xiao-yun; SUN Ke-jun; WANG De-han; LIAO Zong-wen

    2005-01-01

    Fertilizers contribute greatly to high yields but also result in environmental non-point contamination, including the emission of greenhouse gas(N2O) and eutrophication of water bodies. How to solve this problem has become a serious challenge, especially for China as its high ecological pressure. Controlled-release fertilizer(CRF) has been developed to minimize the contamination while keeping high yield and has become a green fertilizer for agriculture. Several CRFs made with special coating technology were used for testing the fertilizer effects in yield and environment through pot experiment and field trial. The result indicated that the CRFs had higher N use efficiency, thus reducing N loss through leaching and volatilization while keeping higher yields. Comparing with imported standard CRFs,the test on CRFs showed similar fertilizer effect but with much lower cost. CRFs application is becoming a new approach for minimizing non-point contamination in agriculture.

  2. NPS Government Purchase Card Program: An Analysis of Internal Controls

    Science.gov (United States)

    2014-03-18

    monthly AO and CH account statements (GSA, n.d.-d). The government has partnerships with Citibank , JP Morgan Chase, and U.S. Bank (GSA, n.d.-b), and... Citibank to process all types of transactions, including those made with the purchase, travel, and fleet card. However, the DoD’s independent agencies also...purchase card training. Furthermore, DoD annual ethics training is required as well. Initial training with Citibank is required, and NPS also requires that

  3. NPS Solar Cell Array Tester Cubesat Flight Testing and Integration

    Science.gov (United States)

    2014-09-01

    front face of NPS-SCAT. The antenna comprises two pieces of piano wire cut to the proper length, stowed using fishing line. Once in orbit, a piece of...launch and the other satellites in the launch vehicle. This is a risk mitigation technique and is performed on every spacecraft large or small...the experiment. Instead the distinction was made after the results were evaluated. As such, there was no previous disposition to which technique

  4. Regulation og non-point phosphorus emissions from the agricultural sector by use of economic incentives

    DEFF Research Database (Denmark)

    Hansen, Line Block

    in the effort to reduce agricultural P runoff. Continued application of P in excess of crop requirements causes P stocks to build up in fields and, over time, this increases the risk of losing P to the aquatic environment through surface runoff and erosion and through leaching via the soil matrix or macropores...... of transporting and applying manure to fields means that increasing mineral-fertilizer prices does not generate a sufficient incentive for farmers to reallocate all P surpluses generated by livestock between farms and fields. The aim of the thesis is to increase the understanding of the long-term impacts....... Once a soil is highly enriched it will become a significant source of P losses for a long time. In Denmark, application of manure is primarily determined by nitrogen (N) crop nutrition, implying that livestock farmers are not motivated to further reduce their surplus P applications. The high costs...

  5. Effects of Climate Change on Non-Point Pollution in Huaibei Region and Comprehensive Regulation Strategies%气候变化对淮北地区面源污染的影响及综合调控

    Institute of Scientific and Technical Information of China (English)

    王刚; 严登华; 尹军; 翁白莎

    2011-01-01

    分析了安徽省淮北地区面源污染的现状与压力,从污染物的产生、迁移、转化与汇聚的机理讨论气候变化对面源污染的影响机制,最后从源头控制、途径控制、末端治理、调控机制建设4个层面提出综合调控措施.%In this study, the situation and pressure of non-point pollution in Huaibei region of Anhui province were analyzed. The influence mechanism of climate change on non-point pollution was discussed from mechanism of generation, migration, conversion, and aggregation of contaminants. In the end, comprehensive coping strategies were put forward from four levels: source control, process control, end treatment and construction of regulation mechanism.

  6. Encapsulation of catechin and epicatechin on BSA NPS improved their stability and antioxidant potential.

    Science.gov (United States)

    Yadav, Ramdhan; Kumar, Dharmesh; Kumari, Avnesh; Yadav, Sudesh Kumar

    2014-01-01

    Nanoencapsulation of antioxidant molecules on protein nanoparticles (NPs) could be an advanced approach for providing stable, better food nutraceuticals and anticancer drugs. The bioavailability and stability of catechin (CAT) and epicatechin (ECAT) were very poor. In the present study, the CAT and ECAT were loaded on bovine serum albumin (BSA) NPs following desolvation method. The transmission electron microscope (TEM) and atomic force microscope (AFM) recorded size of CAT-BSA NPs and ECAT-BSA NPs were 45 ± 5 nm and 48 ± 5 nm respectively. The encapsulation efficiency of CAT and ECAT on BSA NPs was found to be 60.5 and 54.5 % respectively. CAT-BSA NPs and ECAT-BSA NPs show slow and sustained in vitro release. The CAT-BSA NPs and ECAT-BSA NPs were stable in solution at various temperatures 37 °C, 47 °C and 57 °C. DPPH assay revealed that CAT and ECAT maintained their functional activity even after encapsulation on BSA NPs. Furthermore, the efficacy of CAT-BSA NPs and ECAT-BSA NPs determined against A549 cell lines was found to be improved. CAT and ECAT aptly encapsulated in BSA NPs, showed satisfactory sustained release, maintained antioxidant potential and found improved efficacy. This has thus suggested their more effective use in food and nutraceuticals as well as in medical field.

  7. Comparative study of antimicrobial activity of AgBr and Ag nanoparticles (NPs).

    Science.gov (United States)

    Suchomel, Petr; Kvitek, Libor; Panacek, Ales; Prucek, Robert; Hrbac, Jan; Vecerova, Renata; Zboril, Radek

    2015-01-01

    The diverse mechanism of antimicrobial activity of Ag and AgBr nanoparticles against gram-positive and gram-negative bacteria and also against several strains of candida was explored in this study. The AgBr nanoparticles (NPs) were prepared by simple precipitation of silver nitrate by potassium bromide in the presence of stabilizing polymers. The used polymers (PEG, PVP, PVA, and HEC) influence significantly the size of the prepared AgBr NPs dependently on the mode of interaction of polymer with Ag+ ions. Small NPs (diameter of about 60-70 nm) were formed in the presence of the polymer with low interaction as are PEG and HEC, the polymers which interact with Ag+ strongly produce nearly two times bigger NPs (120-130 nm). The prepared AgBr NPs were transformed to Ag NPs by the reduction using NaBH4. The sizes of the produced Ag NPs followed the same trends--the smallest NPs were produced in the presence of PEG and HEC polymers. Prepared AgBr and Ag NPs dispersions were tested for their biological activity. The obtained results of antimicrobial activity of AgBr and Ag NPs are discussed in terms of possible mechanism of the action of these NPs against tested microbial strains. The AgBr NPs are more effective against gram-negative bacteria and tested yeast strains while Ag NPs show the best antibacterial action against gram-positive bacteria strains.

  8. 77 FR 59222 - Proposed Information Collection; Comment Request: NPS Institutional Animal Care and Use Committee...

    Science.gov (United States)

    2012-09-26

    ... National Park Service Proposed Information Collection; Comment Request: NPS Institutional Animal Care and... Submission) used by the Institutional Animal Care and Use Committee (NPS IACUC/the Committee) to ensure... in use without an OMB Control Number. Title: NPS Institutional Animal Care and Use Committee...

  9. SITREP: The NPS Maritime Defense and Security Research Program Newsletter ; v. 38 (April 2009)

    OpenAIRE

    2009-01-01

    This April 2009 issue of the Naval Postgraduate School (NPS) Maritime Defense and Security Research Program Newsletter highlights the following research: "Join MDA [Maritime Defense and Awareness] Outreach on ODNI [Office of the Director of National Intelligence] Bridge" and "NPS Cooperative Operations and Applied Science & Technology Studies." It also provides links to future events, reports, articles, and NPS Theses regarding maritime defense and security.

  10. Controlling the Nanoscale Patterning of AuNPs on Silicon Surfaces

    Directory of Open Access Journals (Sweden)

    Chris J. Allender

    2013-03-01

    Full Text Available This study evaluates the effectiveness of vapour-phase deposition for creating sub-monolayer coverage of aminopropyl triethoxysilane (APTES on silicon in order to exert control over subsequent gold nanoparticle deposition. Surface coverage was evaluated indirectly by observing the extent to which gold nanoparticles (AuNPs deposited onto the modified silicon surface. By varying the distance of the silicon wafer from the APTES source and concentration of APTES in the evaporating media, control over subsequent gold nanoparticle deposition was achievable to an extent. Fine control over AuNP deposition (AuNPs/μm2 however, was best achieved by adjusting the ionic concentration of the AuNP-depositing solution. Furthermore it was demonstrated that although APTES was fully removed from the silicon surface following four hours incubation in water, the gold nanoparticle-amino surface complex was stable under the same conditions. Atomic force microscopy (AFM and X-ray photoelectron spectroscopy (XPS were used to study these affects.

  11. Synthesis of Silver nanoparticles (AgNPs) with Antibacterial Activity

    Science.gov (United States)

    Campillo Gloria, E.; Ederley, Vélez; Gladis, Morales; César, Hincapié; Jaime, Osorio; Oscar, Arnache; Uribe José, Ignacio; Franklin, Jaramillo

    2017-06-01

    The synthesis of nanomaterials is currently one of the most active in nanoscience branches; especially those help improve the human quality life. Silver nanoparticles (AgNPs) are an example of this as it is known to have inhibitory and bactericidal effects. In this work, we report the synthesis of silver nanoparticles by chemical reduction method of silver nitrate (AgNO3) from aqueous solution, using a mix of polivinyl pyrrolidone (PVP) - Aloe Vera as reducing agent and for stabilization and control of particle size. Silver nanoparticles obtained were characterized by Scanning Electron Microscopy (SEM), UV-visible spectroscopy and measurements using Zetasizer Nano ZS were applied to size estimation. The existence of surface plasmon resonance peak at λmax ~ 420 nm is evidence of silver nanoparticles formation. It was possible to standardize an appropriate protocol for the evaluation of bactericidal activity of the nanoparticles, for mesophilic microorganisms. Bactericidal activity above 90% against these kinds of bacteria was demonstrated.

  12. The preparation and antibacterial effects of dopa-cotton/AgNPs

    Energy Technology Data Exchange (ETDEWEB)

    Xu Hong, E-mail: hxu@dhu.edu.cn [Key Lab of Science and Technology of Eco-textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China); Shi Xue; Ma Hui; Lv Yihang; Zhang Linping [Key Lab of Science and Technology of Eco-textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China); Mao Zhiping, E-mail: zhpmao@dhu.edu.cn [Key Lab of Science and Technology of Eco-textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 (China)

    2011-05-15

    Silver nanoparticles (AgNPs) have been known to have powerful antibacterial activity. In this paper, in situ generation of AgNPs on the surface of dopamine modified cotton fabrics (dopa-cotton/AgNPs) in aqueous solution under room temperature is presented. X-ray photoelectron spectroscopy (XPS) and field emission scanning electron microscope (FE-SEM) were used to analyze the surface chemical composition and the morphology of the modified cotton fabrics, respectively. The results indicated that the surface of cotton fabrics was successfully coated with polydopamine and AgNPs. The cotton fabrics with AgNPs showed durable antibacterial activity.

  13. Examining trends in general fecal indicator bacteria and microbial source tracking genetic markers at non-point source impacted Chicago beaches

    Science.gov (United States)

    In the Chicago area, treated wastewater and storm water flow through the engineered Chicago River system to the Mississippi River, with the goal to protect Lake Michigan from urban discharges. Therefore, under dry weather conditions, nearby Lake Michigan recreational beaches shou...

  14. sources

    Directory of Open Access Journals (Sweden)

    Shu-Yin Chiang

    2002-01-01

    Full Text Available In this paper, we study the simplified models of the ATM (Asynchronous Transfer Mode multiplexer network with Bernoulli random traffic sources. Based on the model, the performance measures are analyzed by the different output service schemes.

  15. Study of energy transfer between riboflavin (vitamin B2) and AgNPs

    Science.gov (United States)

    Mokashi, Vidya V.; Walekar, Laxman S.; Anbhule, Prashant V.; Lee, Sang Hak; Patil, Shivajirao R.; Kolekar, Govind B.

    2014-03-01

    Here, we report the studies on the interaction and formation of nanobiocomplex between silver nanoparticle (AgNPs) and vitamin B2, i.e., riboflavin (RF). The binding study of AgNP to RF was studied by fluorescence, UV-Vis, and TEM techniques. AgNPs were prepared by reducing AgNO3 with trisodium citrate. Prepared nanoparticles size obtained at 20 nm having surface Plasmon resonance band at 426 nm. The absorbance band of RF at 264, 374, and 444 nm changes significantly in the presence of AgNPs suggests that there is change in the chemical environment surrounding AgNPs. A fluorescence spectral change for a solution of RF upon the addition of AgNPs and rapid quenching is suggestive of a rapid adsorption of RF on AgNPs.

  16. Enhancement of QDs photoluminescence by localized surface plasmon effect of Au-NPs

    Science.gov (United States)

    Heydari, Esmaeil; Greco, Tonino; Stumpe, Joachim

    2012-04-01

    Photoluminescence enhancement of CdSe/CdS/ZnS QDs by localized surface plasmon resonance of large Au-NPs has been investigated. The photoluminescence of the QDs with an emission wavelength at 620 nm in a PMMA matrix is enhanced by immobilized Au-NPs. By considering the lifetime and excitation dependent photoluminescence we realized that the emission and excitation rate enhancements both contributed to the total photoluminescence enhancement. PL measurements were carried out for different sizes of Au-NPs to find out their influences on the emission of QDs. The largest enhancement is achieved by applying 80 nm Au-NPs. Silanization method gives us the opportunity easily to prepare samples with different concentrations of Au-NPs. It is revealed that increasing the concentration of the Au-NPs layer provides higher scattering cross section which contributes in PL enhancement.

  17. Effects of TiO2 NPs on Silkworm Growth and Feed Efficiency.

    Science.gov (United States)

    Li, YangYang; Ni, Min; Li, FanChi; Zhang, Hua; Xu, KaiZun; Zhao, XiaoMing; Tian, JiangHai; Hu, JingSheng; Wang, BinBin; Shen, WeiDe; Li, Bing

    2016-02-01

    Silkworm (Bombyx mori) (B. mori) is an economically important insect and a model species for Lepidoptera. It has been reported that feeding of low concentrations of titanium dioxide nanoparticles (TiO2 NPs) can improve feed efficiency and increase cocoon mass, cocoon shell mass, and the ratio of cocoon shell. However, high concentrations of TiO2 NPs are toxic. In this study, we fed B. mori with different concentrations of TiO2 NPs (5, 10, 20, 40, 80, and 160 mg/L) and investigated B. mori growth, feed efficiency, and cocoon quality. We found that low concentrations of TiO2 NPs (5 and 10 mg/L) were more effective for weight gains, with significant weight gain being obtained at 72 h (P TiO2 NPs at 20 mg/L or higher had certain inhibitory effects, with significant inhibition to B. mori growth being observed at 48 h. The feed efficiency was significantly improved at low concentrations of 5 and 10 mg/L for 14.6 and 13.1 %, respectively (P TiO2 NPs showed increased cocoon mass and cocoon shell mass; at 5 and 10 mg/L TiO2 NPs, cocoon mass was significantly increased by 8.29 and 9.39 %, respectively (P TiO2 NPs promoted B. mori growth and development, improved feed efficiency, and increased cocoon production, while high concentrations (20 mg/L or higher) of TiO2 NPs showed inhibitory effect to the B. mori. Consecutive feeding of high concentrations of TiO2 NPs led to some degrees of adaptability. This study provides a reference for the research on TiO2 NPs toxicity and the basis for the development of TiO2 NPs as a feed additive for B. mori.

  18. Navy-Wide Personnel Survey (NPS) 2008: Summary of Survey Results

    Science.gov (United States)

    2009-11-01

    officers, enlisted) and, where relevant, comparisons to previous NPS results. The authors acknowledge the assistance of Ms . Evangeline Clewis with the...integration, and organizational commitment. Past NPS and the related Navy Quality of Life ( QOL ) surveys have consistently found that the quality of...psychometric analysis of the 2005 NPS (Bann, Whittam, Barnett- Walker, 2006), in which scales were pared down to their essential questions. In this way, burden

  19. GENERAL REGULARITIES ON NPS CONSTRUCTION CONCRETE CORROSION PROCESSES AND THEIR SIMULATION

    Directory of Open Access Journals (Sweden)

    S. N. Leonovich

    2010-01-01

    Full Text Available Civil engineering material science has accumulated a great amount of scientific data on corrosive processes occurring in the NPS concrete structures (including the Bushehr NPS, Islamic Republic of Iran due to environmental conditions.This practical material creates appropriate prerequisites for generalizations, presentation of results in the form of mathematical models permitting to make calculations on durability of the NPS concrete and reinforced concrete  structures with the required accuracy. 

  20. A comparative analysis of OTF, NPS, and DQE in energy integrating and photon counting digital x-ray detectors.

    Science.gov (United States)

    Acciavatti, Raymond J; Maidment, Andrew D A

    2010-12-01

    One of the benefits of photon counting (PC) detectors over energy integrating (EI) detectors is the absence of many additive noise sources, such as electronic noise and secondary quantum noise. The purpose of this work is to demonstrate that thresholding voltage gains to detect individual x rays actually generates an unexpected source of white noise in photon counters. To distinguish the two detector types, their point spread function (PSF) is interpreted differently. The PSF of the energy integrating detector is treated as a weighting function for counting x rays, while the PSF of the photon counting detector is interpreted as a probability. Although this model ignores some subtleties of real imaging systems, such as scatter and the energy-dependent amplification of secondary quanta in indirect-converting detectors, it is useful for demonstrating fundamental differences between the two detector types. From first principles, the optical transfer function (OTF) is calculated as the continuous Fourier transform of the PSF, the noise power spectra (NPS) is determined by the discrete space Fourier transform (DSFT) of the autocovariance of signal intensity, and the detective quantum efficiency (DQE) is found from combined knowledge of the OTF and NPS. To illustrate the calculation of the transfer functions, the PSF is modeled as the convolution of a Gaussian with the product of rect functions. The Gaussian reflects the blurring of the x-ray converter, while the rect functions model the sampling of the detector. The transfer functions are first calculated assuming outside noise sources such as electronic noise and secondary quantum noise are negligible. It is demonstrated that while OTF is the same for two detector types possessing an equivalent PSF, a frequency-independent (i.e., "white") difference in their NPS exists such that NPS(PC) > or = NPS(EI) and hence DQE(PC) function given as zero or unity everywhere. In analyzing the model detector with Gaussian blurring

  1. The non-point output of different agriculture landuse types in Zhexi hydraulic region of Taihu Basin

    Institute of Scientific and Technical Information of China (English)

    LIHengpeng; LIUXiaomei; HUANGWenyu

    2004-01-01

    This paper takes Zhexi hydraulic region in Taihu Basin as a study area. On the basis of hydraulic analysis function of Arcgis8.3, the drainages were delineated by selecting the monitoring points and discharge stations as outlets. The landuse map were finished by denoting the TM/ETM image. The precipitation map was finished by spatial interpolation according to the rainfall monitoring records. Overlaying the drainage boundary, landuse map and precipitation map, the rainfall, different landuse type area, and runoff pollution concentration and runoff were calculated. Based on these data in different sub-watersheds, by Origin7.0 regression tool, an equation is established to predict runoff using the relationships between runoff, precipitation depth and land use patterns in each of the sub-watersheds. Selecting the sub-watershed which is mainly composed of forest landuse type, the mean runoff concentration (MRC) from sub-watershed has been estimated. The mean runoff concentration of farmland has been estimated by the same methods after the contribution of forestlanduse type was removed. The results are" for the forest landuse type, the mean runoff concentrationsof COD, BOD, Total N and Total P are 2.95 mg/l, 1.080 mg/l, 0.715 mg/l, and 0.039 mg/l,respectively; for the farmland, the mean runoff concentrations of COD, BOD, Total N and Total P are 5.721 rag/l, 3.097 rag/l, 2.092 rag/l, and 0.166 rag/l, respectively. By using these results, the agriculture non-point pollution loads have been assessed. The loads of COD, BOD, Total N and Total P in Zhexi region are 14,631.69 t/a, 6401.93 t/a, 4281.753 t/a and 287.67 t/a, respectively.

  2. 78 FR 9729 - Proposed Information Collection; Comment Request: NPS Institutional Animal Care and Use Committee...

    Science.gov (United States)

    2013-02-11

    ... National Park Service Proposed Information Collection; Comment Request: NPS Institutional Animal Care and... Submission, Exhibitor, Annual Review, and Amendment Forms) used by the Institutional Animal Care and Use... collection is currently in use without an OMB Control Number. Title: NPS Institutional Animal Care and...

  3. Exploring the Attractiveness of New Psychoactive Substances (NPS) among Experienced Drug Users

    NARCIS (Netherlands)

    van Amsterdam, J.G.C.; Nabben, T.; Keiman, D.; Haanschoten, G.; Korf, D.

    2015-01-01

    A growing number of New Psychoactive Substances (NPS) appear yearly on the European market (81 for the first time in 2013, adding to a total of over 350 NPS). Using semi-structured interviews with 25 Dutch experienced recreational drug users, the role of the Internet and friends in gathering and

  4. University Provost Leonard Ferrari Outlines State of NPS During Latest SGL

    OpenAIRE

    Naval Postgraduate School Public Affairs Office

    2011-01-01

    Dr. Leonard Ferrari, NPS Executive Vice President and Provost, offers the State of NPS address to students, staff and faculty on Jan. 18 in King Hall. He spoke of the School’s achievements in 2010 and expressed hope for 2011.

  5. A critical first assessment of the new pre-market approval regime for new psychoactive substances (NPS) in New Zealand.

    Science.gov (United States)

    Wilkins, Chris

    2014-10-01

    New Zealand has recently attempted to address the underlying drivers of the escalating new psychoactive substances (NPS) ('legal highs') problem by establishing the world's first pre-market approval regulatory regime for NPS. NPS products which can be shown with clinical trial data to pose a 'low risk' of harm will be approved for legal manufacture and sale. This paper critically assesses the new regime, drawing on experience of the pharmaceutical sector and legal BZP market. A number of characteristics of the recreational use of NPS may not be well addressed by standard medical clinical trials, including binge use, polydrug use, use by vulnerable groups and high-risk modes of administration. The overt advertising and covert promotion of approved NPS products on the internet may make them fairly visible to young people. The black market for unapproved NPS may be difficult to suppress given that unapproved NPS will be physically identical to approved NPS. If the legal market for NPS encourages the use of NPS, alcohol and other drugs there may be an increase in drug-related harm. Alternatively, if the legal NPS market reduces the use of more harmful drugs, there may be a considerable public health benefit. The clinical trials required for NPS products should address the characteristics of recreational NPS use. Enforcement resources and technical solutions are required to clearly distinguish legal NPS products. The impact the new NPS regime has on other drug use is a key issue and demands further study. © 2014 Society for the Study of Addiction.

  6. Sources

    OpenAIRE

    2015-01-01

    SOURCES MANUSCRITES Archives nationales Rôles de taille 1768/71 Z1G-344/18 Aulnay Z1G-343a/02 Gennevilliers Z1G-340/01 Ivry Z1G-340/05 Orly Z1G-334c/09 Saint-Remy-lès-Chevreuse Z1G-344/18 Sevran Z1G-340/05 Thiais 1779/80 Z1G-391a/18 Aulnay Z1G-380/02 Gennevilliers Z1G-385/01 Ivry Z1G-387b/05 Orly Z1G-388a/09 Saint-Remy-lès-Chevreuse Z1G-391a/18 Sevran Z1G-387b/05 Thiais 1788/89 Z1G-451/18 Aulnay Z1G-452/21 Chennevières Z1G-443b/02 Gennevilliers Z1G-440a/01 Ivry Z1G-452/17 Noiseau Z1G-445b/05 ...

  7. Exploring the Behavior and Metabolic Transformations of SeNPs in Exposed Lactic Acid Bacteria. Effect of Nanoparticles Coating Agent

    Directory of Open Access Journals (Sweden)

    Maria Palomo-Siguero

    2017-08-01

    Full Text Available The behavior and transformation of selenium nanoparticles (SeNPs in living systems such as microorganisms is largely unknown. To address this knowledge gap, we examined the effect of three types of SeNP suspensions toward Lactobacillus delbrueckii subsp. bulgaricus LB-12 using a variety of techniques. SeNPs were synthesized using three types of coating agents (chitosan (CS-SeNPs, hydroxyethyl cellulose (HEC-SeNPs and a non-ionic surfactant, surfynol (ethoxylated-SeNPs. Morphologies of SeNPs were all spherical. Transmission electron microscopy (TEM was used to locate SeNPs in the bacteria. High performance liquid chromatography (HPLC on line coupled to inductively coupled plasma mass spectrometry (ICP-MS was applied to evaluate SeNP transformation by bacteria. Finally, flow cytometry employing the live/dead test and optical density measurements at 600 nm (OD600 were used for evaluating the percentages of bacteria viability when supplementing with SeNPs. Negligible damage was detected by flow cytometry when bacteria were exposed to HEC-SeNPs or CS-SeNPs at a level of 10 μg Se mL−1. In contrast, ethoxylated-SeNPs were found to be the most harmful nanoparticles toward bacteria. CS-SeNPs passed through the membrane without causing damage. Once inside, SeNPs were metabolically transformed to organic selenium compounds. Results evidenced the importance of capping agents when establishing the true behavior of NPs.

  8. Agricultural non-point nitrogen pollution control function of different vegetation types in riparian wetlands: a case study in the Yellow River wetland in China.

    Science.gov (United States)

    Zhao, Tongqian; Xu, Huashan; He, Yuxiao; Tai, Chao; Meng, Hongqi; Zeng, Fanfu; Xing, Menglin

    2009-01-01

    Riparian wetland is the major transition zone of matter, energy and information transfer between aquatic and terrestrial ecosystems and has important functions of water purification and non-point pollution control. Using the field experiment method and an isotope tracing technique, the agricultural non-point nitrogen pollution control function of different vegetation types in riparian wetland was studied in the Kouma Section of the Yellow River. The results showed that the retention of agricultural non-point nitrogen pollution by riparian wetland soil occurs mainly in top 0-10 cm layer. The amount of nitrogen retained by surface soils associated with three types of vegetation are 0.045 mg/g for Phragmites communis Trin Linn, 0.036 mg/g for Scirpus triqueter Linn, and 0.032 mg/g for Typha angustifolia Linn, which account for 59.21%, 56.25%, and 56.14% of the total nitrogen interception, respectively. Exogenous nitrogen in 0-10 cm soil layer changes more quickly than in other layers. One month after adding K(15)NO3 to the tested vegetation, nitrogen content was 77.78% for P. communis Trin, 68.75% for T. angustifolia, and 8.33% for S. triqueter in the surface soil. After three months, nitrogen content was 93.33% for P. communis Trin, 72.22% for S. triqueter, and 37.50% for T. Angustifolia. There are large differences among vegetation communities respecting to purification of agricultural non-point nitrogen pollution. The nitrogen uptake amount decreases in the sequence: new shoots of P. communis Trin (9.731 mg/g) > old P. communis Trin (4.939 mg/g) > S. triqueter (0.620 mg/g) > T. angustifolia (0.186 mg/g). Observations indicated that the presence of riparian wetlands as buffers on and adjacent to stream banks could be recommended to control agricultural non-point pollution.

  9. Agricultural non-point nitrogen pollution control function of different vegetation types in riparian wetlands: A case study in the Yellow River wetland in China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Tongqian; XU Huashan; HE Yuxiao; TAI Chao; MENG Hongqi; ZENG Fanfu; XING Menglin

    2009-01-01

    Riparian wetland is the major transition zone of matter, energy and information transfer between aquatic and terrestrial ecosystems and has important functions of water purification and non-point pollution control. Using the field experiment method and an isotope tracing technique, the agricultural non-point nitrogen pollution control function of different vegetation types in riparian wetland was studied in the Kouma Section of the Yellow River. The results showed that the retention of agricultural non-point nitrogen pollution by riparian wetland soil occurs mainly in top 0-10 cm layer. The amount of nitrogen retained by surface soils associated with three types of vegetation are 0.045 mg/g for Phragmites communis Trin Lima, 0.036 mg/g for Scirpus triqueter Liun, and 0.032 mg/g for Typha angustifolia Linn, which account for 59.21%, 56.25%, and 56.14% of the total nitrogen interception, respectively. Exogenous nitrogen in 0-10 cm soil layer changes more quickly than in other layers. One month after adding K15NO3 to the tested vegetation, nitrogen content was 77.78% for P. Communis Trin, 68.75% for T. Angustifolia, and 8.33% for S. Triqueter in the surface soil. After three months, nitrogen content was 93.33% for P. Communis Trin, 72.22% for S. Triqueter, and 37.50% for T. Angustifolia. There are large differences among vegetation communities respecting to purification of agricultural non-point nitrogen pollution. The nitrogen uptake amount decreases in the sequence: new shoots of P. Communis Trin (9.731 mg/g)>old P. Communis Trin (4.939 mg/g)>S. Triqueter (0.620 mg/g)>T. Angustifolia (0.186 mg/g). Observations indicated that the presence of riparian wetlands as buffers on and adjacent to stream banks could be recommended to control agricultural non-point pollution.

  10. Photocatalytic and antibacterial properties of phytosynthesized CeO2 NPs using Moringa oleifera peel extract.

    Science.gov (United States)

    Surendra, T V; Roopan, Selvaraj Mohana

    2016-08-01

    Biosynthetic methods are alternative approaches which are much safer than the normal techniques (physical and chemical) used for the methods for synthesis of metal nanoparticles. The benefits are sample as it is economic and environment friendly. Herein present investigation, we have reported a microwave mediated eco-friendly synthetic approach for preparing cerium oxide (CeO2) nanoparticles. Here, we used Moringa oleifera peel as the stabilizing and reducing agent towards synthesize of Ce2O NPs via microwave irradiation. The NPs were further characterized using UV-Vis, FT-IR, XRD and HR-TEM techniques. The FTIR analysis confirmed the phytochemical involvement in NPs stabilization. The crystallinity of CeO2 nanoparticles are well demonstrated through X-ray Diffraction and HR-TEM. The TEM images reveal the spherical shape of the CeO2 NPs having an average size of 45nm. Additionally, these CeO2 NPs were used successfully as a catalyst in the degradation of the dye, crystal violet. Also the antibacterial activity of the synthesized CeO2 NPs was evaluated using Staphylococcus aureus (Gram positive bacteria) and Escherichia coli (Gram negative bacteria). CeO2 NPs showed better activity on E. coli than S. aureus. We have demonstrated an eco-friendly preparation of CeO2 nanoparticles, a good photocatalyst and having better antibacterial properties.

  11. Study on Synthesis and Antibacterial Properties of Ag NPs/GO Nanocomposites

    Directory of Open Access Journals (Sweden)

    Lei Huang

    2016-01-01

    Full Text Available Using graphene oxide as substrate and stabilizer for the silver nanoparticles, silver nanoparticles-graphene oxide (Ag NPs/GO composites with different Ag loading were synthesized through a facile solution-phase method. During the synthesis process, AgNO3 on GO matrix was directly reduced by NaBH4. The structure characterization was studied through X-ray diffraction (XRD, atomic force microscopy (AFM, high-resolution transmission electron microscope (HRTEM, ultraviolet-visible spectroscopy (UV-Vis, and selected area electron diffraction (SAED. The results show that Ag nanoparticles (Ag NPs with the sizes ranging from 5 to 20 nm are highly dispersed on the surfaces of GO sheets. The shape and size of the Ag NPs are decided by the volume of initial AgNO3 solution added in the GO. The antibacterial activities of Ag NPs/GO nanocomposites were investigated and the result shows that all the produced composites exhibit good antibacterial activities against Gram-negative (G− bacterial strain Escherichia coli (E. coli and Gram-positive (G+ strain Staphylococcus aureus (S. aureus. Moreover, the antibacterial activities of Ag NPs/GO nanocomposites gradually increased with the increasing of volume of initial AgNO3 solution added in the GO and this improvement of the antibacterial activities results from the combined action of size effect and concentration effect of Ag NPs in Ag NPs/GO nanocomposites.

  12. Green synthesized conditions impacting on the reactivity of Fe NPs for the degradation of malachite green

    Science.gov (United States)

    Huang, Lanlan; Luo, Fang; Chen, Zuliang; Megharaj, Mallavarapu; Naidu, Ravendra

    2015-02-01

    This study investigates green tea extract synthesized conditions impacting on the reactivity of iron nanoparticles (Fe NPs) used for the degradation of malachite green (MG), including the volume ratio of Fe2+ and tea extract, the solution pH and temperature. Results indicated that the reactivity of Fe NPs increased with higher temperature, but fell with increasing pH and the volume ratio of Fe2+ and tea extract. Scanning electron microscope (SEM), energy-dispersive spectrometer (EDS), Fourier transform infrared spectroscope (FTIR) and X-ray diffraction (XRD) indicated that Fe NPs were spherical in shape, their diameter was 70-80 nm and they were mainly composed of iron oxide nanoparticles. UV-visible (UV-vis) indicated that reactivity of Fe NPs used in degradation of MG significantly depended on the synthesized conditions of Fe NPs. This was due to their impact on the reactivity and morphology of Fe NPs. Finally, degradation of MG showed that 90.56% of MG was removed using Fe NPs.

  13. Design of NPS61000SHEV Extended Range Electric Bus%NPS6100SHEV增程式电动客车设计

    Institute of Scientific and Technical Information of China (English)

    徐本祥

    2011-01-01

    Through the power matching, generator-set matching, battery capacity calculation and control strategy analyzing of NPS6100SHEV extended range electric bus, the author puts forward the overall design requirement and general design method of the extended range electric bus.%通过对NPS6100SHEV增程式电动客车的动力匹配、发电机组匹配、电池容量计算和控制策略分析,提出增程式电动客车的总体设计要求和一般设计方法。

  14. Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs

    Science.gov (United States)

    Wang, Yu; Khan, Altaz; Som, Madhura; Leigh, Steven Y.; Wang, Danni; Chen, Ye; McVeigh, Patrick; Wilson, Brian C.; Liu, Jonathan T. C.

    2014-05-01

    We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted to cell-surface biomarkers in fresh tissues. Ex vivo and in vivo experiments were performed to optimize our strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5 min) topical application of SERS NPs on tissues. The simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations, potentially enabling point-of-care surgical guidance or early disease detection.

  15. Synthesis and optical properties study of nanocomposites based on AuNPs and AgNPs obtained by laser ablation in liquid monomer

    Science.gov (United States)

    Zulina, Natalia A.; Pavlovetc, Ilia M.; Baranov, Mikhail A.; Kaliabin, Viacheslav O.; Denisyuk, Igor Yu.

    2017-01-01

    In this work, Ag and Au nanoparticles (AgNPs and AuNPs) stable colloid solution were prepared by laser ablation of chemically pure silver rod and gold film in liquid monomer isodecyl acrylate (IDA). Sizes of obtained nanoparticles were determined by scanning electron microscope and vary from 30 to 130 nm. Nanocomposites films were prepared from obtained stable colloid solution by photocuring. To prepare solid film based on aliphatic polymer IDA long molecules cross-linking, 1,6-Hexandiol diacrylate was used. Obtained films were transparent, homogenous and colored in brown for AgNPs and purple for AuNPs-filled nanocomposites. Prepared nanomaterials exhibit strong third-order nonlinear optical responses under CW laser irradiation at 532 nm, which was estimated by using z-scan technique performed with open and close aperture. The real and imaginary parts of third-order nonlinear optical coefficients of the studied nanocomposites were found to be up to Reχ (3) = 1.31 × 10-5 (esu) and Imχ (3) = 7.64 × 10-5 (esu), for samples negative nonlinear refractive index was obtained, which corresponds to self-defocusing effect.

  16. Key Player in Maersk Alabama Hostage Rescue Addresses NPS Students During Guest Lecture

    OpenAIRE

    Naval Postgraduate School Public Affairs Office

    2012-01-01

    Renowned Naval Postgraduate School (NPS) alumnus and former USS Bainbridge (DDG-96) commander, U.S. Navy Capt. Frank Castellano, recently addressed students and faculty at NPS’ King Auditorium, Oct. 16.

  17. Effect of silver NPs plasmon on optical properties of fluorescein dye

    Science.gov (United States)

    Ragab, Alaa EL-din E. A.; Gadallah, A.; Mohamed, Mona B.; Azzouz, I. M.

    2013-11-01

    In this work we studied the effect of silver nanoparticles "AgNPs" on the optical properties of fluoretain-->rescein dye. Fluorescein dye solutions have been mixed with different concentrations of colloidal AgNPs. Absorption and fluorescence enhancement of fluorescein dye molecules was detected in the presence of AgNPs. Fluorescence enhancement of the dye molecules was observed with a maximal enhancement factor of about 3-fold. Enhancement of the rate of radiative transition was also detected. The enhancement mechanisms are attributed to a modification of the local density of electromagnetic modes in the vicinity of AgNPs at energies resonant with surface Plasmon. The ability of fluorophore-metal mixture to actively enhance the dye's luminescence could leads to new opportunities for technological development of light emitting and photonic devices. It also may have applications in the fields of bio-technology and medical diagnostics as new class of fluorescence based sensing.

  18. Fabrication LSPR sensor chip of Ag NPs and their biosensor application based on interparticle coupling

    Energy Technology Data Exchange (ETDEWEB)

    Ghodselahi, T., E-mail: t_ghodselahi@yahoo.com [Nano Mabna Iranian Inc., PO Box 1676664116, Tehran (Iran, Islamic Republic of); School of Physics, Institute for Research in Fundamental Sciences, PO Box 19395-5531, Tehran (Iran, Islamic Republic of); Neishaboorynejad, T. [School of Physics, Institute for Research in Fundamental Sciences, PO Box 19395-5531, Tehran (Iran, Islamic Republic of); Department of Physics, Central Tehran Branch, Islamic Azad University, Tehran (Iran, Islamic Republic of); Arsalani, S. [School of Physics, Institute for Research in Fundamental Sciences, PO Box 19395-5531, Tehran (Iran, Islamic Republic of); School of Medicine, Bam University of Medical Sciences, Bam (Iran, Islamic Republic of)

    2015-07-15

    Highlights: • Localized surface plasmon resonance (LSPR) sensor of silver nanoparticles on hydrogenated amorphous carbon thin film were synthetized by co-deposition of RF-sputtering and RF-PECVD. • Samples were characterized by XRD, XPS, AFM, and UV visible. • DNA primer at fM concentration was detected based on breaking of inter-particles coupling. • Dipolar plasmon of isolated Ag NPs, coupled Ag NPs plasmons, in-plane and out-plane coupling, and quadrupole plasmon modes were considered to explain biosensor properties. • The initial response, wavelength shift sensitivity, and response time of LSPR sensors were compared by morphology. - Abstract: We introduce a simple method to synthesize localized surface plasmon resonance (LSPR) sensor chip of Ag NPs on the hydrogenated amorphous carbon by co-deposition of RF-Sputtering and RF-PECVD. The X-ray photoelectron spectroscopy revealed the content of Ag and C atoms. X-ray diffraction profile and atomic force microscopy indicate that the Ag NPs have fcc crystal structure and spherical shape and by increasing deposition time, particle sizes do not vary and only Ag NPs aggregation occurs, resulting in LSPR wavelength shift. Firstly, by increasing Ag NPs content, in-plan interparticles coupling is dominant and causes redshift in LSPR. At the early stage of agglomeration, out-plane coupling occurs and in-plane coupling is reduced, resulting a blueshift in the LSPR. By further increasing of Ag NPs content, agglomeration is completed on the substrate and in-plan coupling rises, resulting significant redshift in the LSPR. Results were used to implement biosensor application of chips. Detection of DNA primer at fM concentration was achieved based on breaking interparticles coupling of Ag NPs. A significant wavelength shift sensitivity of 30 nm and a short response time of 30 min were obtained, where both of these are prerequisite for biosensor applications.

  19. Enhanced photocatalytic performance of morphologically tuned Bi2S3 NPs in the degradation of organic pollutants under visible light irradiation.

    Science.gov (United States)

    Sarkar, Arpita; Ghosh, Abhisek Brata; Saha, Namrata; Srivastava, Divesh N; Paul, Parimal; Adhikary, Bibhutosh

    2016-12-01

    Here in, morphologically tuned Bi2S3 NPs were successfully synthesized from a single-source precursor complex [Bi(ACDA)3] [HACDA=2-aminocyclopentene-1-dithiocarboxylic acid] by decomposing in various solvents using a simple solvothermal method. The as-obtained products were characterized by XRD, TEM, UV-vis spectroscopy and BET surface area measurements. Structural analyses revealed that the as-prepared Bi2S3 NPs can be tuned to different morphologies by varying various solvents and surfactants. The interplay of factors that influenced the size and morphology of the nanomaterials has been studied. Moreover, mastery over the morphology of nanoparticles enables control of their properties and enhancement of their usefulness for a given application. These materials emerged as a highly active visible light-driven photocatalyst towards degradation of methylene blue dye and the efficiencies are dependent on size and surface area of the NPs. In addition, photocatalytic degradation of highly toxic dichlorodiphenyltrichloroethane was studied using synthesized Bi2S3 NPs as catalyst and the rate of degradation has been found to be much better compared to that exhibited by commercial WO3. We believe that this new synthesis approach can be extended to the synthesis of other metal sulfide nanostructures and open new opportunities for device applications.

  20. Assessing the influence of different plant species in drainage ditches on mitigation of non-point source pollutants (N, P, and sediments) in the Purple Sichuan Basin.

    Science.gov (United States)

    Kumwimba, Mathieu Nsenga; Zhu, Bo; Muyembe, Diana Kavidia

    2017-06-01

    Three different types of ditches, each 300 m in length, were employed in this study. One vegetated constructed ditch (VCD), three natural vegetated soil ditches (NVSD), and three constructed ditches left unvegetated (UCD) as controls were investigated using simple in/out mass balances and uptake by plant species with a potential for phytoremediation and their mechanisms. Significant differences in the ditches were observed, suggesting the importance of plant species in nutrient mitigation. The removal rates of TN (total nitrogen) and TP (total phosphorus) were 64.28 and 58.02, 31.16 and 27.49, and 3.91 and 2.97%, respectively, in the VCD, NVSD, and UCD. Canna indica (45.12 g m(-2)) and Oenanthe javanica (21.48 g m(-2)) had the highest total N and P storage in the VCD and NVSD. Furthermore, species C. indica possessed the highest annual N and P uptake in the VCD (216.59 kg N/ha/yr and 30.73 kg P/ha/yr). In the NVSD, species O. javanica had the greatest annual N and P uptake (96.66 kg N/ha/yr and 7.94 kg P/ha/yr). Both VCD and NVSD were found to have a reasonably good outcome compared to UCD. Retention of nutrients by ditch sediments was probably the major attenuation mechanism, with subsequent plant uptake and microbial nitrification-denitrification of the nutrients as secondary removal mechanisms. Results of this study highlight the importance of taking actions for establishment of appropriate plant species inside the ditches in order to enhance its direct and indirect roles and maximize purification rate in aquatic environments.

  1. Development and application of a coupled bio-geochmical and hydrological model for point and non-point source river water pollution

    Science.gov (United States)

    Pohlert, T.

    2007-12-01

    The aim of this paper is to present recent developments of an integrated water- and N-balance model for the assessment of land use changes on water and N-fluxes for meso-scale river catchments. The semi-distributed water-balance model SWAT was coupled with algorithms of the bio-geochemical model DNDC as well as the model CropSyst. The new model that is further denoted as SWAT-N was tested with leaching data from a long- term lysimeter experiment as well as results from a 5-years sampling campaign that was conducted at the outlet of the meso-scale catchment of the River Dill (Germany). The model efficiency for N-load as well as the spatial representation of N-load along the river channel that was tested with results taken from longitudinal profiles show that the accuracy of the model has improved due to the integration of the aforementioned process-oriented models. After model development and model testing, SWAT-N was then used for the assessment of the EU agricultural policy (CAP reform) on land use change and consequent changes on N-fluxes within the Dill Catchment. giessen.de/geb/volltexte/2007/4531/

  2. Bibliometric analysis on literatures of Non-Point Source Pollution at A Watershed Scale%流域非点源污染文献计量分析

    Institute of Scientific and Technical Information of China (English)

    马鑫; 闫铁柱; 曹洪涛; 陈少波

    2014-01-01

    非点源污染成为制约中国社会经济和环境可持续发展的重要因素.为了准确掌握流域非点源污染研究的全球状况和前沿动态,客观反映相关国家或地区在该领域的科学能力和科学影响,采用ISI Web of Knowlere的Web of Science引文数据库,选用基本检索方式,以流域非点源的相关术语作为主题检索词,对1900年-2012年之间该库收录的此领域的相关文献进行计量分析.结果表明:从发文量、研究机构和研究人员、高被引文献分析可以看出,美国在此领域成绩卓越,领先于其他国家;在典型机理模型运用方面,SWAT的发文量最多,占模型运用相关文献发文量的52%;国际上该领域最主要的期刊有Journal of The American Water Resources Association、Science of The Total Environment、Journal of Hydrology和Journal of Environmental Quality;中国科学院、北京师范大学和浙江大学的流域非点源污染领域发文量在中国位居前3位.

  3. AnnAGNPS – A United States Department of Agriculture Watershed Conservation Management Planning Tool for Non-Point Source Pollution Control

    Science.gov (United States)

    A watershed scale assessment of the effect of conservation practices on the environment is critical when recommending best management practices to agricultural producers. The environmental benefits of these practices have not been widely quantified at the watershed scale, which would require extens...

  4. Governing change: land-use change and the prevention of nonpoint source pollution in the north coastal basin of California.

    Science.gov (United States)

    Short, Anne G

    2013-01-01

    Many rural areas in the United States and throughout much of the postindustrial world are undergoing significant ecological, socioeconomic, and political transformations. The migration of urban and suburban dwellers into rural areas has led to the subdivision of large tracts of land into smaller parcels, which can complicate efforts to govern human-environmental problems. Non-point source (NPS) pollution from private rural lands is a particularly pressing human-environmental challenge that may be aggravated by changing land tenure. In this article, I report on a study of the governance and management of sediment (a common NPS pollutant) in the North Coastal basin of California, a region undergoing a transition from traditional extractive and agricultural land uses to rural residential and other alternative land uses. I focus on the differences in the governance and management across private timber, ranch, residential, vacation, and other lands in the region. I find that (1) the stringency and strength of sediment regulations differ by land use, (2) nonregulatory programs tend to target working landscapes, and (3) rural residential landowners have less knowledge of sediment control and report using fewer sediment-control techniques than landowners using their land for timber production or ranching. I conclude with an exploration of the consequences of these differences on an evolving rural landscape.

  5. Hepatoma-Targeted Radionuclide Immune Albumin Nanospheres: (131)I-antiAFPMcAb-GCV-BSA-NPs.

    Science.gov (United States)

    Lin, Mei; Huang, Junxing; Zhang, Dongsheng; Jiang, Xingmao; Zhang, Jia; Yu, Hong; Xiao, Yanhong; Shi, Yujuan; Guo, Ting

    2016-01-01

    An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres ((131)I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of (131)I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of (131)I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of (131)I alone. As well, the uptake rate and retention ratios of (131)I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells. Compared to (131)I alone, (131)I-antiAFPMcAb-GCV-BSA-NPs were much more easily taken in and retained by hepatoma tissue, with a much higher T/NT. Due to good drug-loading, high encapsulation ratio, and highly selective affinity for AFP-positive tumors, the (131)I-antiAFPMcAb-GCV-BSA-NPs are promising for further effective radiation-gene therapy of hepatoma.

  6. Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs.

    Science.gov (United States)

    Jamshidi, Mitra; Ghanati, Faezeh; Rezaei, Ayatollah; Bemani, Ebrahim

    2016-05-01

    Elicitation effect of silver nano particles (AgNPs) and triggering of defence system by production of hydrogen peroxide (H2O2) as a signaling molecule in the regulation of the activity of stress-related enzymes and production of Taxol was evaluated in suspension- cultured hazel cells (Corylus avellana L.). The cells were treated with different concentrations of AgNPs (0, 2.5, 5, and 10 ppm), in their logarithmic growth phase (d7) and were harvested after 1 week. Treatment of hazel cells with AgNPs decreased the viability of the cells. Also the results showed that while the activity of certain radical scavenging enzymes in particular of catalase and peroxidase increased by 2.5 and 5 ppm AgNPs, the activity of superoxide dismutase decreased in these treatments. The highest activity of ascorbate peroxidase was observed in 10 ppm AgNPs treatments. This treatment also showed the highest contents of H2O2 and phenolic compounds, as well as the highest activity of phenylalanine ammonialyase. According to the results, 5 ppm AgNPs was the best concentration for elicitation of hazel cells to produce efficient amounts of H2O2 in order for stimulation of antioxidant defence system, production of Taxol at the highest capacity of the cells, meanwhile reserving their viability.

  7. Evaluation of genotoxic effect of silver nanoparticles (Ag-Nps) in vitro and in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Tavares, Priscila; Balbinot, Fernanda; Martins de Oliveira, Hugo; Elibio Fagundes, Gabriela [PPGCS, Universidade do Extremo Sul Catarinense, Laboratorio de Biologia Celular e Molecular (Brazil); Venancio, Mireli; Vieira Ronconi, Joao Vitor; Merlini, Aline [Universidade do Extremo Sul Catarinense, Laboratorio de Sintese de Complexos Multifuncionais (Brazil); Streck, Emilio L. [Programa de Pos-Graduacao em Ciencias da Saude, Unidade Academica de Ciencias da Saude, Universidade do Extremo Sul Catarinense, Laboratorio de Fisiopatologia Experimental (Brazil); Marques da Silva, Paula [Universidade do Extremo Sul Catarinense, Laboratorio de Sintese de Complexos Multifuncionais (Brazil); Moraes de Andrade, Vanessa, E-mail: vmoraesdeandrade@yahoo.com.br [PPGCS, Universidade do Extremo Sul Catarinense, Laboratorio de Biologia Celular e Molecular (Brazil)

    2012-03-15

    Silver nanoparticles (Ag-NPs) are the most prominent nanoproducts. Due to their antimicrobial activity, they have been incorporated in different materials, such as catheters, clothes, electric home appliance, and many others. The genotoxicity of Ag-NPs (5-45 nm), in different concentrations and times of exposure, was evaluated by the comet assay in in vitro and in vivo conditions, respectively, using human peripheral blood and Swiss mice. The results showed the genotoxic effect of Ag-NPs in vitro, in all the doses tested in the initial hour of exposure, possibly through the reactive oxygen species generation. Nevertheless, the values for this damage decrease with time, indicating that the DNA may have been restored by the repair system. In the in vivo conditions, we found no genotoxicity of Ag-NPs in any hour of exposure and any dose investigated, which can be attributed to the activation of a cellular antioxidant network and the hydrophobic nature of Ag-NPs. Now, it is absolutely necessary to investigate the role of Ag-NPs in different cell lines in vivo.

  8. Lexical Link Analysis for the Haiti Earthquake Relief Operation Using Open Data Sources

    Science.gov (United States)

    2011-06-01

    Information Systems Experimentation ( DISE ) Group Naval Postgraduate School, Monterey, CA 93943 yzhao@nps.edu, spgallup@nps.edu, djmackin@nps.edu...Using Open Data Sources Dr. Ying Zhao, Dr. Shelley P. Gallup, and Dr. Douglas J. MacKinnon Distributed Information Systems Experimentation ( DISE ...Proceedings of the Seventh Annual Acquisition Research Program. Monterey, CA: Naval Postgraduate School. DISE Distributed Information Systems

  9. Histopathological effects of nanosilver (Ag-NPs in liver after dermal exposure during wound healing

    Directory of Open Access Journals (Sweden)

    Mohammad Saeed Heydarnejad

    2014-04-01

    Full Text Available   Objective(s: With the advent of nanotechnology, significant progress has been made in the area of nanoscale materials such as nanosilver (Ag-Nps. These nanoparticles have a wide range of applications and been used for antimicrobial purposes for more than a century. However, little attention has been paid to the toxicity of nanosilver wound dressing. This study was designed to investigate the possible histopathological toxicity of Ag-NPs in liver of mice during wound healing.     Materials and Methods:   A group of 50 female BALB/c mice of about 8 weeks were randomly divided into two groups: Ag-NPs and control groups (n=25. After creating similar wound on the backs of all animals, the wound bed was treated in Ag-NPs group, with a volume of 50 microliters of the nanosilver solution (10ppm ,and in control group, with the same amount of distilled water. The experiment lasted for 14 days. Histopathaological samplings of liver were conducted on days 2, 7 and 14 of the experiment.   Results: Histopathological studies demonstrated time-dependent changes in mice liver treated with Ag-NPs compared to control group. Some changes include dilation in central venous, hyperemia, cell swelling, increase of Kupffer and inflammatory cells. Conclusion: This study suggests that use of nanosilver for wound healing may cause a mild toxicity, as indicated by time-dependent toxic responses in liver tissue. However, this issue will have to be considered more extensively in further studies.

  10. Green technology for durable finishing of viscose fibers via self-formation of AuNPs.

    Science.gov (United States)

    Emam, Hossam E; El-Hawary, Nancy S; Ahmed, Hanan B

    2017-03-01

    Sensitivity of dyes' colors to the surrounding environment causes lower durability and stability of color, which reflects the importance of durable finishing treatment. Current technique offered antimicrobial/durable finishing of viscose fibers through direct formation of AuNPs inside fibers macromolecules without using any external agents. By using the reducing properties of cellulose in viscose, Au(+3) was reduced to AuNPs and CHO/OH of cellulose subsequently were oxidized to COOH. For comparison, two different media were used; aqueous and alkaline. Increasing the reactivity and accessibility of cellulose macromolecules in alkali leaded to enlargement of the reduction process and more incorporation of AuNPs. Size of AuNPs inside fiber was recorded to be in range of 22-112nm and 14-100nm, in case of using aqueous and alkaline medium, respectively. Structure and properties of fibers were not changed by treatment according to XRD and ATR-FTIR data. The treated fibers were acquired durable violet color by the action of LSPR for AuNPs and darker color obtained using higher Au(+3) concentration. The treated fibers exhibited good inhibition against different pathogenic microbes including bacteria and fungi. One-pot, quite simple, inexpensive, green and industrial viable are the significant advantages of the current technique for viscose finishing (pigmentation and antimicrobial action). Copyright © 2016 Elsevier B.V. All rights reserved.

  11. The Interactions between ZnO Nanoparticles (NPs and α-Linolenic Acid (LNA Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Yiwei Zhou

    2017-04-01

    Full Text Available Background: Nanoparticles (NPs entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA and NPs. Methods: This study used α-linolenic acid (LNA complexed to bovine serum albumin (BSA for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results: The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis, fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells, associated with an increase of intracellular Zn ions, but not intracellular superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-6 was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA was present. The presence of LNA did not significantly affect all of these endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from the present study indicated that BSA-complexed LNA might modestly interact with ZnO NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in HepG2 cells.

  12. TEA controllable preparation of magnetite nanoparticles (Fe3O4 NPs) with excellent magnetic properties

    Science.gov (United States)

    Han, Chengliang; Zhu, Dejie; Wu, Hanzhao; Li, Yao; Cheng, Lu; Hu, Kunhong

    2016-06-01

    A fast and controllable synthesis method for superparamagnetic magnetite nanoparticles (Fe3O4 NPs) was developed in Fe(III)-triethanolamine (TEA) solution. The phase structure, morphology and particle size of the as-synthesized samples were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results showed that the magnetic particles were pure Fe3O4 with mean sizes of approximately 10 nm. The used TEA has key effects on the formation of well dispersing Fe3O4 NPs. Vibrating sample magnetometer (VSM) result indicated that the as-obtained Fe3O4 NPs exhibited superparamagnetic behavior and the saturation magnetization (Ms) was about 70 emu/g, which had potential applications in magnetic science and technology.

  13. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems.

    Science.gov (United States)

    Abdelgawad, Abdelrahman M; Hudson, Samuel M; Rojas, Orlando J

    2014-01-16

    Novel hybrid nanomaterials have been developed for antimicrobial applications. Here we introduce a green route to produce antibacterial nanofiber mats loaded with silver nanoparticles (Ag-NPs, 25 nm diameter) enveloped in chitosan after reduction with glucose. The nanofiber mats were obtained from colloidal dispersions of chitosan-based Ag-NPs blended with polyvinyl alcohol. Nanofibers (150 nm average diameter and narrow size distribution) were obtained by electrospinning and cross-linked with glutaraldhyde. The effect of crosslinking on the release of silver was studied by atomic absorption spectroscopy. Antimicrobial activity was studied by the viable cell-counting; mats loaded with silver and control samples (chitosan/PVA) with different degrees of cross-linking were compared for their effectiveness in reducing or halting the growth of aerobic bacteria. The results showed superior properties and synergistic antibacterial effects by combining chitosan with Ag-NPs.

  14. Bifunctional Nanoparticle-SILP Catalysts (NPs@SILP) for the Selective Deoxygenation of Biomass Substrates

    Energy Technology Data Exchange (ETDEWEB)

    Luska, Kylie L. [RWTH Aachen Univ. (Germany); Julis, Jennifer [RWTH Aachen Univ. (Germany); Evonik Industries AG, Marl (Germany); Stavitski, Eli [Brookhaven National Lab. (BNL), Upton, NY (United States); Zakharov, Dmitri N. [Brookhaven National Lab. (BNL), Upton, NY (United States); Adams, Alina [RWTH Aachen Univ. (Germany); Leitner, Walter [RWTH Aachen Univ. (Germany); Max Planck Inst. for Coal Research, Ruhr (Germany)

    2014-08-27

    We immobilized ruthenium nanoparticles onto an acidic supported ionic liquid phase (RuNPs@SILP) in the development of bifunctional catalysts for the selective deoxygenation of biomass substrates. RuNPs@SILPs possessed high catalytic activities, selectivities and recyclabilities in the hydrogenolytic deoxygenation and ring opening of C8- and C9-substrates derived from furfural or 5-hydroxymethylfurfural and acetone. When we tailor the acidity of the SILP through the ionic liquid loading provided a molecular parameter by which the catalytic activity and selectivity of the RuNPs@SILPs were controlled to provide a flexible catalyst system toward the formation of different classes of value-added products: cyclic ethers, primary alcohols or aliphatic ethers.

  15. Application of a Multi-Objective Optimization Method to Provide Least Cost Alternatives for NPS Pollution Control

    Science.gov (United States)

    Maringanti, Chetan; Chaubey, Indrajeet; Arabi, Mazdak; Engel, Bernard

    2011-09-01

    Nonpoint source (NPS) pollutants such as phosphorus, nitrogen, sediment, and pesticides are the foremost sources of water contamination in many of the water bodies in the Midwestern agricultural watersheds. This problem is expected to increase in the future with the increasing demand to provide corn as grain or stover for biofuel production. Best management practices (BMPs) have been proven to effectively reduce the NPS pollutant loads from agricultural areas. However, in a watershed with multiple farms and multiple BMPs feasible for implementation, it becomes a daunting task to choose a right combination of BMPs that provide maximum pollution reduction for least implementation costs. Multi-objective algorithms capable of searching from a large number of solutions are required to meet the given watershed management objectives. Genetic algorithms have been the most popular optimization algorithms for the BMP selection and placement. However, previous BMP optimization models did not study pesticide which is very commonly used in corn areas. Also, with corn stover being projected as a viable alternative for biofuel production there might be unintended consequences of the reduced residue in the corn fields on water quality. Therefore, there is a need to study the impact of different levels of residue management in combination with other BMPs at a watershed scale. In this research the following BMPs were selected for placement in the watershed: (a) residue management, (b) filter strips, (c) parallel terraces, (d) contour farming, and (e) tillage. We present a novel method of combing different NPS pollutants into a single objective function, which, along with the net costs, were used as the two objective functions during optimization. In this study we used BMP tool, a database that contains the pollution reduction and cost information of different BMPs under consideration which provides pollutant loads during optimization. The BMP optimization was performed using a NSGA

  16. Concentration-dependent, size-independent toxicity of citrate capped AuNPs in Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Giuseppe Vecchio

    Full Text Available The expected potential benefits promised by nanotechnology in various fields have led to a rapid increase of the presence of engineered nanomaterials in a high number of commercial goods. This is generating increasing questions about possible risks for human health and environment, due to the lack of an in-depth assessment of the physical/chemical factors responsible for their toxic effects. In this work, we evaluated the toxicity of monodisperse citrate-capped gold nanoparticles (AuNPs of different sizes (5, 15, 40, and 80 nm in the model organism Drosophila melanogaster, upon ingestion. To properly evaluate and distinguish the possible dose- and/or size-dependent toxicity of the AuNPs, we performed a thorough assessment of their biological effects, using two different dose-metrics. In the first approach, we kept constant the total surface area of the differently sized AuNPs (Total Exposed Surface area approach, TES, while, in the second approach, we used the same number concentration of the four different sizes of AuNPs (Total Number of Nanoparticles approach, TNN. We observed a significant AuNPs-induced toxicity in vivo, namely a strong reduction of Drosophila lifespan and fertility performance, presence of DNA fragmentation, as well as a significant modification in the expression levels of genes involved in stress responses, DNA damage recognition and apoptosis pathway. Interestingly, we found that, within the investigated experimental conditions, the toxic effects in the exposed organisms were directly related to the concentration of the AuNPs administered, irrespective of their size.

  17. Green Synthesis of AgNPs Stabilized with biowaste and their antimicrobial activities

    Directory of Open Access Journals (Sweden)

    Nakuleshwar Dut Jasuja

    2014-12-01

    Full Text Available In the present study, rapid reduction and stabilization of Ag+ ions with different NaOH molar concentration (0.5 mM, 1.0 mM and 1.5 mM has been carried out in the aqueous solution of silver nitrate by the bio waste peel extract of P.granatum. Generally, chemical methods used for the synthesis of AgNPs are quite toxic, flammable and have adverse effect in medical application but green synthesis is a better option due to eco-friendliness, non-toxicity and safe for human. Stable AgNPs were synthesized by treating 90 mL aqueous solution of 2 mM AgNO3 with the 5 mL plant peels extract (0.4% w/v at different NaOH concentration (5 mL. The synthesized AgNPs were characterized by UV-Vis spectroscopy, TEM and SEM. Further, antimicrobial activities of AgNPs were performed on Gram positive i.e. Staphylococcus aureus, Bacillus subtilius and Gram negative i.e. E. coli, Pseudomonas aeruginosa bacteria. The AgNPs synthesized at 1.5 mM NaOH concentration had shown maximum zone of inhibition (ZOI i.e. 49 ± 0.64 in E. coli, whereas Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilius had shown 40 ± 0.29 mm, 28 ± 0.13 and 42 ± 0.49 mm ZOI respectively. The MIC value of 30 g/mL observed for E. coli Whereas, Staphylococcus aureus, Bacillus subtilius and Pseudomonas aeruginosa had shown 45 µg/mL, 38 µg/mL, 35 µg/mL respectively. The study revealed that AgNPs had shown significant antimicrobial activity as compared to Streptomycin.

  18. Facile fabrication of AgNPs/(PVA/PEI) nanofibers: high electrochemical efficiency and durability for biosensors.

    Science.gov (United States)

    Zhu, Han; Du, MingLiang; Zhang, Ming; Wang, Pan; Bao, ShiYong; Wang, LiNa; Fu, YaQin; Yao, JuMing

    2013-11-15

    A novel, facile and green approach for the fabrication of H2O2, glutathione (GSH) and glucose detection biosensor using water-stable PVA and PVA/PEI nanofibers decorated with AgNPs by combining an in situ reduction approach and electrospinning technique has been demonstrated. Small, uniform and well-dispersed AgNPs embedded in the PVA nanofibers and immobilized on functionalized PVA/PEI nanofibers indicate the highly sensitive detection of H2O2 with a detection limit of 5 μM and exhibit a fast response, broad linear range, low detection limit and excellent stability and reusability.

  19. NPS State Vector Analysis and Relative Motion Plotting Software for STS-51. Appendix C

    Science.gov (United States)

    1994-03-01

    vector *’tans -in, State -vector ftans-out, * double tans buf, int fbi, int point num, Perturbations *P) *This filter, created by Lou Zyla is designed to...main menu; ups menu displayWntO; return YES; static void ups display graphics(int graph mode) void upsdisplay~ grphics (int graph mode) if (nps ub~plot...in~graphics mode = YES; else if (ups graphics info.in graphics-mode = = NO) return; closegrapho; ups graphics info. in~ grphics mode =NO; void nps

  20. Influence of particle coating and matrix constituents on the cloud point extraction efficiency of silver nanoparticles (Ag-NPs) and application for monitoring the formation of Ag-NPs from Ag(+).

    Science.gov (United States)

    Hartmann, Georg; Baumgartner, Tanja; Schuster, Michael

    2014-01-01

    For the quantification of silver nanoparticles (Ag-NPs) in environmental samples using cloud point extraction (CPE) for selective enrichment, surface modification of the Ag-NPs and matrix effects can play a key role. In this work we validate CPE with respect to the influence of different coatings and naturally occurring matrix components. The Ag-NPs tested were functionalized with inorganic and organic compounds as well as with biomolecules. Commercially available NPs and NPs synthesized according to methods published in the literature were used. We found that CPE can extract almost all Ag-NPs tested with very good efficiencies (82-105%). Only Ag-NPs functionalized with BSA (bovine serum albumin), which is a protein with the function to keep colloids in solution, cannot be extracted. No or little effect of environmentally relevant salts, organic matter, and inorganic colloids on the CPE of AgNPs was found. Additionally we used CPE to observe the in situ formation of Ag-NPs produced by the reduction of Ag(+) with natural organic matter (NOM).

  1. Green synthesis of AuNPs for eco-friendly functionalization of cellulosic substrates

    Science.gov (United States)

    Ibrahim, Nabil A.; Eid, Basma M.; Abdel-Aziz, Mohamed S.

    2016-12-01

    In this research work, extracellular biosynthesis of gold nanoparticles (AuNPs) using marine bacterial isolates (Streptomyces sp.) as a reducing/capping/stabilizing bio-agent and chlolauric acid (HAuCl4) as a precursor has been investigated. Surface modification of cotton and viscose knitted fabrics using O2-plasma followed by subsequent treatment with bio-synthesized AuNPs alone and in combination with TiO2NPs or ZnONPs to impart new functional properties namely antibacterial and UV-blocking were studied. The results show that loading of nominated nanomaterials onto the activated fabric samples results in a significant improvement in antibacterial activity against both G+ve (S. aureus) and G-ve (E. coli) along with a remarkable enhancement in the UV-protection functionality of the treated fabrics. The highest antibacterial and anti-UV values were obtained when O2-plasma treated fabrics were loaded with AuNPs/ZnONPs combination, irrespective of the used substrate. The imparted functional properties demonstrated remarkable retention even after 15 washings.

  2. 76 FR 62090 - Public Meeting for the National Park Service (NPS) Alaska Region's Subsistence Resource...

    Science.gov (United States)

    2011-10-06

    ... Education. 4. Climate Change Research. b. Subsistence Manager. c. Resource Management. 1. Wildlife (Musk Ox, Brown Bear, Sheep). 2. NPS Research/Studies. 3. Ranger Report (Education, Outreach and Visitor Protection). 9. Federal Subsistence Board Update. 10. Alaska Board of Game Update. 11. Old Business....

  3. New Tools for Characterizing Metallic Nanoparticles: AgNPs, A Case Study.

    Science.gov (United States)

    González-Fuenzalida, Rodrigo A; Moliner-Martínez, Yolanda; Molins-Legua, Carmen; Parada-Artigues, Vanesa; Verdú-Andrés, Jorge; Campins-Falcó, Pilar

    2016-01-19

    Currently, transmission electron microscopy (TEM) is the main technique for estimating the sizes of spherical nanoparticles (NPs) and through them, their concentrations. This paper demonstrates for the first time that C18 reversed-phase capillary liquid chromatography (Cap-LC) coupled to diode array detection (DAD) has the potential to estimate mean concentrations of silver nanoparticles (AgNPs) and thereby determine their average size. Direct injection of the sample without previous extraction or separation steps is carried out. Only a unique standard with a known AgNP size is needed for the calibration. In a first approach, the new method has been tested over silver nanoparticles, produced using different methods of synthesis, and their water dilutions. Good results were achieved: relative errors ranged up to 5% compared with TEM. Also stability and functionality-related NP properties, as well as nonspherical AgNPs, can be studied using this method. Moreover, by coupling online in-tube solid-phase microextraction (IT-SPME) to Cap-LC-DAD, the effect of the dilution can be studied as particles distribute by polarity in two groups, a distribution that responds to average particle size of not only AgNPs, but also gold nanoparticles (AuNPs). In such a distribution, the average particle size is correlated with the peak area ratio. Additionally, besides higher sensitivity and concentration-dependent signals, IT-SPME-Cap-LC responds to changes in the particle's hydrodynamic diameter allowing, for instance, the detection of cationic surfactants. Size-exclusion and hydrophobic effects are the mechanisms involved to explain this behavior.

  4. Modification of Thin Film Composite (TFC) Membrane by Incorporation with Copper Nanoparticles (Cu-NPs) for Antibacterial Properties

    Science.gov (United States)

    Zhong, Chen

    Membrane biofouling has been a challenging problem restricting the application of reverse osmosis (RO) desalination process. Copper is known for its antimicrobial properties and is easily available with low cost. In this paper, copper nanoparticles (Cu-NPs) with a mean diameter of 15nm were synthesized by the reduction of copper (II) chloride with sodium borohydride (NaBH4), using cetyl trimethylammonium bromide ((C16H33)N(CH3) 3Br, CTAB) as a capping agent. After purification of Cu-NPs by dialysis, the particles were successfully immobilized onto the surface of thin film composite (TFC) membranes via either electrostatic interactions or by covalent bonding with cysteamine as a linker. The electrostatic method was simply to immerse the newly made TFC membranes to the Cu-NPs suspension. Since the CTAB had formed cationic bilayer outside the Cu-NPs, the Cu-NPs was not only adsorbed on the membranes but also attached to the surface because of the electrostatic effect with the negatively charged membrane surface. The covalent bonding method utilized cysteamine (C4H12N2S 2) to activate the thin film layer with thiol functional groups first and then incorporated the metallic copper nanoparticles to form the stable covalent chemical bonding in between. The resulting membranes by these two methods were labeled as TFC-CuNPs and TFC-S-CuNPs, respectively, in this study. Scanning electron microscopy (SEM) imaging and associated energy-dispersive X-ray spectroscopy (EDS) showed that large amounts of Cu-NPs existed on both types of membranes. Surface hydrophilicity of the membranes was enhanced by the presence of Cu-NPs, as indicated by the measured contact angle of 63.25 +/- 0.75 for TFC, 38.63 +/- 2.16 for TFC-CuNPs, and 58.00 +/- 3.39 for TFC-S-CuNPs. Consistently, the water flux obtained from the RO desalination system was increased from 47.07 +/- 0.84 for TFC, 49.10 +/- 0.22 for TFC-CuNPs, and 69.13 +/- 1.43 for TFC-S-CuNPs, with this increase in hydrophilicity. The salt

  5. Study of fungicidal properties of colloidal silver nanoparticles (AgNPs on trout egg pathogen, Saprolegnia sp.

    Directory of Open Access Journals (Sweden)

    Seyed Ali Johari

    2015-05-01

    Full Text Available Silver nanoparticles (AgNPs are known to have bactericidal and fungicidal effects. Since, there is few information available on the interaction of colloidal nanosilver with fish pathogens. Hence, the current study investigated the effects of colloidal AgNPs on the in vitro growth of the fish pathogen Saprolegnia sp.. Before the experiments, various important properties of AgNPs were well-characterized. The antifungal activity of AgNPs was then evaluated by determining the minimum inhibitory concentrations (MICs using two-fold serial dilutions of colloidal nanosilver in a glucose yeast extract agar at 22ºC. The growth of Saprolegnia sp. on the AgNPs agar treatments was compared to that of nanosilver-free agar as controls. The results showed that AgNPs have an inhibitory effect on the in vitro growth of the tested fungi. The MIC of AgNPs for Saprolegnia sp. was calculated at 1800 mg/L, which is equal to 0.18 percent. It seems that AgNPs could be a proper replacement for teratogenic and toxic agents, such as malachite green. In addition, the indirect use of AgNPs could be a useful method for providing new antifungal activity in aquaculture systems.

  6. Understanding the risks associated with the use of new psychoactive substances (NPS): high variability of active ingredients concentration, mislabelled preparations, multiple psychoactive substances in single products.

    Science.gov (United States)

    Zamengo, Luca; Frison, Giampietro; Bettin, Chiara; Sciarrone, Rocco

    2014-08-17

    New psychoactive substances (NPS), are now a large group of substances of abuse not yet completely controlled by international drug conventions, which may pose a public health threat. Anxiety, paranoia, hallucinations, seizures, hyperthermia and cardiotoxicity are some of the common adverse effects associated with these compounds. In this paper, three case reports taken from the archive of processed cases of the authors' laboratory are presented and discussed to stress the risks of possible adverse consequences for NPS users: in particular, (i) the risk deriving from the difficulty of predicting the actual consumed dose, due to variability of active ingredients concentration in consumed products, (ii) the risk deriving from the difficulty of predicting the actual active ingredients present in consumed products, as opposed to those claimed by the manufacturer, and (iii) the risk deriving from the difficulty of predicting the actual pharmacological and toxicological effects related to the simultaneous consumption of different psychoactive ingredients contained in single products, whose interactions are mostly unknown. Each of them individually provide a source of concern for possible serious health related consequences. However, they should be considered in conjunction with each others, with the worldwide availability of NPS through the web and also with the incessantly growing business derived from the manipulation and synthesis of new substances. The resulting scenario is that of a cultural challenge which demands a global approach from different fields of knowledge.

  7. Molecular control of TiO₂-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins.

    Directory of Open Access Journals (Sweden)

    Yinxia Li

    Full Text Available With growing concerns of the safety of nanotechnology, the in vivo toxicity of nanoparticles (NPs at environmental relevant concentrations has drawn increasing attentions. We investigated the possible molecular mechanisms of titanium nanoparticles (Ti-NPs in the induction of toxicity at predicted environmental relevant concentrations. In nematodes, small sizes (4 nm and 10 nm of TiO₂-NPs induced more severe toxicities than large sizes (60 nm and 90 nm of TiO₂-NPs on animals using lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and reactive oxygen species (ROS production as endpoints. Locomotion behaviors could be significantly decreased by exposure to 4-nm and 10-nm TiO₂-NPs at concentration of 1 ng/L in nematodes. Among genes required for the control of oxidative stress, only the expression patterns of sod-2 and sod-3 genes encoding Mn-SODs in animals exposed to small sizes TiO₂-NPs were significantly different from those in animals exposed to large sizes of TiO₂-NPs. sod-2 and sod-3 gene expressions were closely correlated with lethality, growth, reproduction, locomotion behavior, intestinal autofluorescence, and ROS production in TiO₂-NPs-exposed animals. Ectopically expression of human and nematode Mn-SODs genes effectively prevented the induction of ROS production and the development of toxicity of TiO₂-NPs. Therefore, the altered expression patterns of Mn-SODs may explain the toxicity formation for different sizes of TiO₂-NPs at predicted environmental relevant concentrations. In addition, we demonstrated here a strategy to investigate the toxicological effects of exposure to NPs upon humans by generating transgenic strains in nematodes for specific human genes.

  8. Dynorphin-dependent reduction of excitability and attenuation of inhibitory afferents of NPS neurons in the pericoerulear region of mice

    Directory of Open Access Journals (Sweden)

    Kay eJuengling

    2016-03-01

    Full Text Available The Neuropeptide S system, consisting of the 20-amino acid peptide neuropeptide S (NPS and its G-protein coupled receptor (NPSR, modulates arousal, wakefulness, anxiety, and fear-extinction in mice. In addition, recent evidence indicates that the NPS system attenuates stress-dependent impairment of fear extinction, and that NPS-expressing neurons in close proximity to the locus coeruleus (pericoerulear, periLC region are activated by stress. Furthermore, periLC NPS neurons receive afferents from neurons of the centrolateral nucleus of the amygdala (CeL, of which a substantial population expresses the kappa opioid receptor (KOR ligand precursor prodynorphin. This study aims to identify the effect of the dynorphinergic system on NPS neurons in the periLC via pre- and postsynaptic mechanisms. Using electrophysiological recordings in mouse brain slices, we provide evidence that NPS neurons in the periLC region are directly inhibited by dynorphin A via activation of κ-opioid receptor 1 (KOR1 and a subsequent increase of potassium conductances. Thus, the dynorphinergic system is suited to inactivate NPS neurons in the periLC. In addition to this direct, somatic effect, dynorphin A reduces the efficacy of GABAergic synapses on NPS neurons via KOR1 and KOR2. In conclusion, the present study provides evidence for the interaction of the NPS and the kappa opioid system in the periLC. Therefore, the endogenous opioid dynorphin is suited to inhibit NPS neurons with a subsequent decrease in NPS release in putative target regions leading to a variety of physiological consequences such as increased anxiety or vulnerability to stress exposure.

  9. Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1.

    Science.gov (United States)

    Yamamoto, Kenta; Nishimura, Munehiro; Kato, Dai-ichiro; Takeo, Masahiro; Negoro, Seiji

    2011-06-01

    4-Nitrophenol (4-NP) is a toxic compound formed in soil by the hydrolysis of organophosphorous pesticides, such as parathion. We previously reported the presence of the 4-NP degradation gene cluster (nphRA1A2) in Rhodococcus sp. strain PN1, which encodes a two-component 4-NP hydroxylase system that oxidizes 4-NP into 4-nitrocatechol. In the current study, another gene cluster (npsC and npsRA2A1B) encoding a similar 4-NP hydroxylase system was cloned from strain PN1. The enzymes from this 4-NP hydroxylase system (NpsA1 and NpsA2) were purified as histidine-tagged (His-) proteins and then characterized. His-NpsA2 showed NADH/FAD oxidoreductase activity, and His-NpsA1 showed 4-NP oxidizing activity in the presence of His-NpsA2. In the 4-NP oxidation using the reconstituted enzyme system (His-NpsA1 and His-NpsA2), hydroquinone (35% of 4-NP disappeared) and hydroxyquinol (59% of 4-NP disappeared) were detected in the presence of ascorbic acid as a reducing reagent, suggesting that, without the reducing reagent, 4-NP was converted into their oxidized forms, 1,4-benzoquinone and 2-hydroxy-1,4-benzoquinone. In addition, in the cell extract of recombinant Escherichia coli expressing npsB, a typical spectral change showing conversion of hydroxyquinol into maleylacetate was observed. These results indicate that this nps gene cluster, in addition to the nph gene cluster, is also involved in 4-NP degradation in strain PN1.

  10. GIS-based predicative modelling of agricultural non point pollution. A case study; Modellazione previsionale GIS-based dell'inquinamento di origine diffusa da fonti agricole. Un caso di studio

    Energy Technology Data Exchange (ETDEWEB)

    Pistocchi, A. [Studio di Ingegneria per l' Ambiente e il Territorio, Cesena (Italy); Bettini, S.; Miserocchi, R. [Geaprogetti Ambiente e Territorio, Ravenna (Italy)

    2000-01-01

    A mathematical modelling approach is presented for agricultural non point source pollution, based on a GIS paradigm. The capability of GIS technology is exploited so to achieve a detailed description of the study area via simple mathematical relations. The features that make the approach preferable for land use management and planning are highlighted. An application to a case study is also given that shows how the model can be set up using currently available cartography. [Italian] Viene presentato un approccio alla modellazione matematica dell'inquinamento di origine diffusa da fonti agricole, che si basa sul paradigma dei sistemi informativi geografici e sulla possibilita' di descrivere i fenomeni idrologici mediante relazioni matematiche semplici e una quantita' di dati capillarmente distribuiti. Vengono messe in evidenza le caratteristiche che rendono questo tipo di approccio preferibile alla modellazione classica per quanto riguarda la pianificazione territoriale. Si illustra l'applicazione al caso di studio del bacino del fiume Savio in Emilia Romagna, mostrando l'applicabilita' del metodo a partire dalle conoscenze rappresentate nella cartografia corrente.

  11. Biological studies and electrical conductivity of paper sheet based on PANI/PS/Ag-NPs nanocomposite.

    Science.gov (United States)

    Youssef, A M; Mohamed, S A; Abdel-Aziz, M S; Abdel-Aziz, M E; Turky, G; Kamel, S

    2016-08-20

    Polyaniline (PANI) with/without polystyrene (PS), was successfully manufactured in the occurrence of dispersed pulp fibers via the oxidative polymerization reaction of aniline monomer to produce conductive paper sheets containing PANI, PANI/PS composites. Additionally, sliver nitrate (Ag-NO3) was added by varied loadings to the oxidative polymerization of aniline monomer to provide sliver nanoparticles (Ag-NPs) emptied into the prepared paper sheets. The prepared paper sheets were examined using scanning electron microscopy (SEM), X-ray diffraction (XRD) and infrared spectroscopy (IR), the mechanical properties of the prepared paper sheets were evaluated. Moreover, the electrical conductivity and biological studies such as cellulases assay, Microorganism & culture condition and detection of the released of Ag-NPs were evaluated. Furthermore, the prepared paper sheets were displayed good antibacterial properties contrary to gram positive and gram negative bacteria. Consequently, the prepared paper sheet may be used as novel materials for packaging applications.

  12. Monitoring new psychoactive substances (NPS) in The Netherlands : data from the drug market and the Poisons Information Centre

    NARCIS (Netherlands)

    Hondebrink, Laura|info:eu-repo/dai/nl/304833134; Nugteren-van Lonkhuyzen, Annette; Van Der Gouwe, Daan; Brunt, Tibor M

    2015-01-01

    BACKGROUND: In recent years, the number of new psychoactive substances (NPS) appearing on the illicit drug market strongly increased. However, little is known about their toxic effects and risks. Therefore, we determined the most frequently occurring NPS in The Netherlands and combined this with dat

  13. Monitoring new psychoactive substances (NPS) in The Netherlands : data from the drug market and the Poisons Information Centre

    NARCIS (Netherlands)

    Hondebrink, Laura; Nugteren-van Lonkhuyzen, Annette; Van Der Gouwe, Daan; Brunt, Tibor M

    2015-01-01

    BACKGROUND: In recent years, the number of new psychoactive substances (NPS) appearing on the illicit drug market strongly increased. However, little is known about their toxic effects and risks. Therefore, we determined the most frequently occurring NPS in The Netherlands and combined this with

  14. Monitoring new psychoactive substances (NPS) in The Netherlands : data from the drug market and the Poisons Information Centre

    NARCIS (Netherlands)

    Hondebrink, Laura; Nugteren-van Lonkhuyzen, Annette; Van Der Gouwe, Daan; Brunt, Tibor M

    2015-01-01

    BACKGROUND: In recent years, the number of new psychoactive substances (NPS) appearing on the illicit drug market strongly increased. However, little is known about their toxic effects and risks. Therefore, we determined the most frequently occurring NPS in The Netherlands and combined this with dat

  15. The Information Revolution Planning for Institutional Change, The NPS Information Technology Strategic Plan / FY 2003

    OpenAIRE

    2003-01-01

    The Joint Vision of NPS outlines the future goals for the institution within the context of national priorities and defines academic goals through excellence in scholarly accomplishment and peer recognition. It requires that our faculty and staff are experienced in using modern technologies for teaching and learning. We must nurture and sustain efforts in high performance computing, multi-media technology, educational technology, and the technology of distance education.

  16. Low-Speed Wind Tunnel Testing of the NPS/NASA Ames Mach 6 Optimized Waverider

    Science.gov (United States)

    1994-06-16

    Specifically, thanks to Prof. Richard M. Howard for providing wind tunnel equipment and guidance necessary to run the tests. Thanks to Mr. Jack King for...tuft in the column. However, each tuft was mounted perpendicular to the centerline. Professor Richard Howard, NPS Department of Aeronautical and...axial.arrayl(), sone.arrmyl() COMMON SHARF .? stwo.Arrayl(), rm.arraylf() 72 DECLARE S"P volt (nonol, nftwn, axisa], sonel, Rtwol, rml) SCREEN 9, 0 CAIIPRATION

  17. Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs

    Science.gov (United States)

    Qiu, Yuan; Rojas, Elena; Murray, Richard A.; Irigoyen, Joseba; Gregurec, Danijela; Castro-Hartmann, Pablo; Fledderman, Jana; Estrela-Lopis, Irina; Donath, Edwin; Moya, Sergio E.

    2015-04-01

    Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties.Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell

  18. Photocurrent Enhancement of P3HT:PCBM Organic Solar Cell with Cylindrical Ag-NPs by EBM.

    Science.gov (United States)

    Park, Gye-Choon

    2015-08-01

    The power conversion efficiency (PCE) of PSS/P3HT:PCBM organic solar cells(SCs) with Ag nanoparticles (NPs) on ITO is studied, and the optical absorption of the SCs with a cylindrical Ag-NPs model is investigated. The fabricated device structure is simulated with a finite difference time domain (FDTD) method. This cylindrical Ag-NPs model is able to explain the photocurrent enhancement by the localized surface plasmon resonance (LSPR) modes, and to provide a further understanding of Ag-NPs shape parameters which play an important role to determine the broadband absorption phenomena in plasmonic organic solar cells. Compared with the SCs without Ag-NPs, an increase in the PCE of the plasmonic solar cell was accurately identified. The photocurrent-voltage characteristic of the examined solar cells reveals higher efficiency of 2.75% compared with the reference cells which show 1.67%.

  19. Chronic ZnO-NPs exposure at environmentally relevant concentrations results in metabolic and locomotive toxicities in Caenorhabditis elegans.

    Science.gov (United States)

    Huang, Chi-Wei; Li, Shang-Wei; Hsiu-Chuan Liao, Vivian

    2017-01-01

    ZnO nanoparticles (ZnO-NPs) are emerging contaminants that raise the concerns of potential risk in the aquatic environment. It has been estimated that the environmental ZnO-NPs concentration is 76 μg/l in the aquatic environment. Our aim was to determine the aquatic toxicity of ZnO-NPs with chronic exposure at environmentally relevant concentrations using the nematode Caenorhabditis elegans. Two simulated environmentally relevant mediums-moderately hard reconstituted water (EPA water) and simulated soil pore water (SSPW)-were used to represent surface water and pore water in sediment, respectively. The results showed that the ZnO-NPs in EPA water has a much smaller hydrodynamic diameter than that in SSPW. Although the ionic release of Zn ions increased time-dependently in both mediums, the Zn ions concentrations in EPA water increased two-fold more than that in SSPW at 48 h and 72 h. The ZnO-NPs did not induce growth defects or decrease head thrashes in C. elegans in either media. However, chronic exposure to ZnO-NPs caused a significant reduction in C. elegans body bends in EPA water even with a relatively low concentration (0.05 μg/l); similar results were not observed in SSPW. Moreover, at the same concentrations (50 and 500 μg/l), body bends in C. elegans were reduced more severely in ZnO-NPs than in ZnCl2 in EPA water. The ATP levels were consistently and significantly decreased, and ROS was induced after ZnO-NPs exposure (50 and 500 μg/l) in EPA water. Our results provide evidences that chronic exposure to ZnO-NPs under environmentally relevant concentrations causes metabolic and locomotive toxicities implicating the potential ecotoxicity of ZnO-NPs at low concentrations in aquatic environments.

  20. Rapid Biosynthesis of AgNPs Using Soil Bacterium Azotobacter vinelandii With Promising Antioxidant and Antibacterial Activities for Biomedical Applications

    Science.gov (United States)

    Karunakaran, Gopalu; Jagathambal, Matheswaran; Gusev, Alexander; Torres, Juan Antonio Lopez; Kolesnikov, Evgeny; Kuznetsov, Denis

    2017-07-01

    Silver nanoparticles (AgNPs) are applied in various fields from electronics to biomedical applications as a result of their high surface-to-volume ratio. Even though different approaches are available for synthesis of AgNPs, a nontoxic method for the synthesis has not yet been developed. Thus, this study focused on developing an easy and ecofriendly approach to synthesize AgNPs using Azotobacter vinelandii culture extracts. The biosynthesized nanoparticles were further characterized by ultraviolet-visible (UV-Vis) spectroscopy, x-ray diffraction (XRD), Fourier transform infrared (FTIR), energy-dispersive spectrum, particle size distribution (PSD), and transmission electron microscopy (TEM). UV absorption noticed at 435 nm showed formation of AgNPs. The XRD pattern showed a face-centered cubic structure with broad peaks of 28.2°, 32.6°, 46.6°, 55.2°, 57.9°, and 67.8°. The FTIR confirmed the involvement of various functional groups in the biosynthesis of AgNPs. The PSD and TEM analyses showed spherical, well-distributed nanoparticles with an average size of 20-70 nm. The elemental studies confirmed the existence of pure AgNPs. The bacterial extract containing extracellular enzyme nitrate reductase converted silver nitrate into AgNPs. AgNPs significantly inhibited the growth of pathogenic bacteria such as Streptomyces fradiae (National Collection of Industrial Microorganisms (NCIM) 2419), Staphylococcus aureus (NCIM 2127), Escherichia coli (NCIM 2065), and Serratia marcescens (NCIM 2919). In addition, biosynthesized AgNPs were found to possess strong antioxidant activity. Thus, the results of this study revealed that biosynthesized AgNPs could serve as a lead in the development of nanomedicine.

  1. Rapid Biosynthesis of AgNPs Using Soil Bacterium Azotobacter vinelandii With Promising Antioxidant and Antibacterial Activities for Biomedical Applications

    Science.gov (United States)

    Karunakaran, Gopalu; Jagathambal, Matheswaran; Gusev, Alexander; Torres, Juan Antonio Lopez; Kolesnikov, Evgeny; Kuznetsov, Denis

    2016-11-01

    Silver nanoparticles (AgNPs) are applied in various fields from electronics to biomedical applications as a result of their high surface-to-volume ratio. Even though different approaches are available for synthesis of AgNPs, a nontoxic method for the synthesis has not yet been developed. Thus, this study focused on developing an easy and ecofriendly approach to synthesize AgNPs using Azotobacter vinelandii culture extracts. The biosynthesized nanoparticles were further characterized by ultraviolet-visible (UV-Vis) spectroscopy, x-ray diffraction (XRD), Fourier transform infrared (FTIR), energy-dispersive spectrum, particle size distribution (PSD), and transmission electron microscopy (TEM). UV absorption noticed at 435 nm showed formation of AgNPs. The XRD pattern showed a face-centered cubic structure with broad peaks of 28.2°, 32.6°, 46.6°, 55.2°, 57.9°, and 67.8°. The FTIR confirmed the involvement of various functional groups in the biosynthesis of AgNPs. The PSD and TEM analyses showed spherical, well-distributed nanoparticles with an average size of 20-70 nm. The elemental studies confirmed the existence of pure AgNPs. The bacterial extract containing extracellular enzyme nitrate reductase converted silver nitrate into AgNPs. AgNPs significantly inhibited the growth of pathogenic bacteria such as Streptomyces fradiae (National Collection of Industrial Microorganisms (NCIM) 2419), Staphylococcus aureus (NCIM 2127), Escherichia coli (NCIM 2065), and Serratia marcescens (NCIM 2919). In addition, biosynthesized AgNPs were found to possess strong antioxidant activity. Thus, the results of this study revealed that biosynthesized AgNPs could serve as a lead in the development of nanomedicine.

  2. n vivo retention of ingested Au NPs by Daphnia magna: No evidence for trans-epithelial alimentary uptake

    Science.gov (United States)

    Khan, Farhan R.; Kennaway, Gabrielle M.; Croteau, Marie-Noële; Dybowska, Agnieszka; Smith, Brian D.; Nogueira, António J.A.; Rainbow, Philip S.; Luoma, Samuel N.; Valsami-Jones, Eugenia

    2014-01-01

    In vivo studies with Daphnia magna remain inconclusive as to whether engineered nanoparticles (NPs) are internalized into tissues after ingestion. Here we used a three-pronged approach to study the in vivo retention and efflux kinetics of 20 nm citrate stabilized Au NPs ingested by this key aquatic species. Daphnids were exposed to suspended particles (600 μg L−1) for 5 h after which they were depurated for 24 h in clean water containing algae. Light microscopy was used to follow the passage of Au NPs through the gastrointestinal tract, Au body burdens were determined by ICP-MS (inductively coupled plasma mass spectrometry), and transmission electron microscopy (TEM) was used to examine the presence and distribution of Au NPs in tissues. Results revealed that the elimination of Au NPs was bi-phasic. The fast elimination phase lasted −1 (±SE) which accounted for ∼75% of the ingested Au. The remaining ∼25% of the ingested Au NPs was eliminated at a 100-fold slower rate. TEM analysis revealed that Au NPs in the midgut were in close proximity to the peritrophic membrane after 1 and 24 h of depuration. There were no observations of Au NP uptake at the microvilli. Thus, although Au NPs were retained in the gut lumen, there was no observable internalization into the gut epithelial cells. Similar to carbon nanotubes and CuO NPs, our findings indicate that in daphnids the in vivo retention of Au NPs does not necessarily result in their internalization.

  3. 城市化进程中武昌沙湖流域面源污染负荷时空分布模拟%Simulation of spatial temporal distribution of NPS loadings in Shahu Lake watershed during process of urbanization

    Institute of Scientific and Technical Information of China (English)

    曾庆慧; 庄艳华; 洪松

    2012-01-01

    The paper uses TM remote sensing data to classify the land use type of Shahu Lake watershed and uses cellular automata model to forecast the transformation of land use type from 2011 to 2015;and then conbining combine the forecast results with non-point source pollution model to simulate temporal variation and spatial distribution of NPS Loadings of Shahu Lake in 2015.The results are as follows: During 2003-2010,urban area was expanding continuously,other types of land use were decreasing at a speed of: farmland 56.8%,greenland 35.1%,water area 23.9%,barren land 8.9.During 2011-2015,the other types of land use have the trend to transform into urban area.From the volume dose variation of NPS pollutants we can see that: compared with 2003,the volume dose of these three pollutants had increased substantially by 2015;the increasing speed are: TN 30.11%,TP 17.32%,BOD 43.38%.From the spatial distribution of NPS pollutants loadings we can see that: in 2003,the spatial distribution of TN was urban area greenland farmland barren land,while in 2015,the regularity of distribution becomes urban area barren land farmland greenland;in 2015,the spatial distribution of TP and BOD are the same with 2003;the regularity of distribution are urban area barren land farmland greenland.%利用遥感影像图进行武昌沙湖流域的土地利用分类,利用元胞自动机(CA)模型预测2011-2015年的土地利用变化,将CA预测结果与面源模型相结合,预测2015年沙湖流域面源污染物总氮(TN)、总磷(TP)、生化需氧量(BOD)负荷的时空分布.研究结果表明2003-2010年间,建设用地面积不断扩展,其他地类向建设用地转变速度依次为:农田56.8%、绿地35.1%、水域23.9%、未利用地8.9%.2011-2015年间各用地类型均有继续向建设用地转变的趋势.从污染物负荷总量的变化情况上看,2015年3种污染物的负荷总量较2003年均有较大幅度增长,其中:TN增长30.11%,TP增长17.32%,BOD

  4. Study on the Effect of γ-Irradiation on Gadolinium Oxysulfide Nanophosphors (Gd2O2S-NPs

    Directory of Open Access Journals (Sweden)

    Muhammad Hassyakirin Hasim

    2017-01-01

    Full Text Available Gadolinium oxysulfide nanophosphors (Gd2O2S-NPs have been successfully synthesized using γ-irradiation and hydrogenation treatment. The primary stage of Gd2O2S-NPs synthesis was carried out using various doses of γ-irradiation to form diverse sizes of Gd2(SO43 precursor, followed by hydrogenation treatment at 900°C for 2 hours to form Gd2O2S-NPs. Then, the nanophosphors were characterized for the structure, morphology, and luminescence properties through X-ray diffraction (XRD, field emission scanning electron microscopy (FESEM, and photoluminescence spectrometer (PL. Pure hexagonal phase of Gd2O2S-NPs was obtained with high crystallinity and without any impurities. The morphologies were observed from grain-like nanostructures transformed to spherical shape as the irradiation dose reached 40 kGy. Besides, Gd2O2S-NPs which were prepared at highest irradiation dose of 40 kGy show highest intensity of emission peak at 548 nm and corresponded to Stark level transition from the GJ6 state of Gd3+ ion. It can be emphasized that the different doses of γ-irradiation influenced the nucleation event of Gd2(SO43 precursor thus affecting the morphology and size particles of Gd2O2S-NPs. Hence, from the results, it can be suggested that Gd2O2S-NPs can be a promising host for optical applications.

  5. Structural, chemical and optical properties of SnO2 NPs obtained by three different synthesis routes

    Science.gov (United States)

    Drzymała, Elżbieta; Gruzeł, Grzegorz; Depciuch, Joanna; Budziak, Andrzej; Kowal, Andrzej; Parlinska-Wojtan, Magdalena

    2017-08-01

    Polyol (P), chemical precipitation (C) and microwave-assisted (M) syntheses were chosen to produce SnO2 nanoparticles with uniform size and minimum agglomeration. Their structural, chemical and optical properties were investigated using dynamic light scattering (DLS), scanning transmission electron microscopy (STEM), Raman, Fourier Transform Infrared (FTIR) using the Attenuated Total Reflectance (ATR) technique and Ultraviolet-Visible (UV-Vis) spectroscopies. STEM observations showed that the SnO2(P) and SnO2(C) nanoparticles (NPs) are combined into larger agglomerates with heterogeneous thickness, while the microwave-assisted NPs form a uniform thin layer across the TEM grid. The strongest agglomeration of the SnO2(C) NPs, observed by DLS, STEM and UV-Vis is explained by the very moderate amount of water present on the surface of the NPs identified by FTIR spectroscopy. High resolution STEM combined with SAED and X-ray diffraction (XRD) patterns confirmed the crystalline character of the NPs. In the nanoparticles from polyol synthesis, chlorine from the remains of metal precursors during reduction was detected by energy dispersive spectroscopy (EDS), contrary to the NPs obtained by the chemical precipitation and microwave-assisted methods. All three syntheses routes lead to small, 2-10 nm SnO2 NPs, which were the result of the low concentration of Cl ions in the solutions.

  6. Ratiometric fluorescence detection of superoxide anion based on AuNPs-BSA@Tb/GMP nanoscale coordination polymers.

    Science.gov (United States)

    Liu, Nan; Hao, Juan; Cai, Keying; Zeng, Mulan; Huang, Zhenzhong; Chen, Lili; Peng, Bingxian; Li, Ping; Wang, Li; Song, Yonghai

    2017-08-03

    A novel ratiometric fluorescence nanosensor for superoxide anion (O2(•-) ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb(3+) and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O2(•-) . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O2(•-) demonstrated high sensitivity and selectivity with a wide linear response range (14 nM-10 μM) and a low detection limit (4.7 nM). Copyright © 2017 John Wiley & Sons, Ltd.

  7. The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

    Science.gov (United States)

    Urbańska, Kaja; Pająk, Beata; Orzechowski, Arkadiusz; Sokołowska, Justyna; Grodzik, Marta; Sawosz, Ewa; Szmidt, Maciej; Sysa, Paweł

    2015-03-01

    Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated. Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

  8. Study on Management and Control of Nonpoint Source Pollution from Urban Surface Runoff

    Institute of Scientific and Technical Information of China (English)

    Chen Keliang; Zhu Xiaodong; Wang Xianghua; Ma Yan

    2007-01-01

    Urbanization is the dominant form of land-use change in terms of impacts on water quality, hydrology, physical properties of watersheds and their nonpoint source (NPS) pollution potential at present. Urbanization has changed the source, process and sink of urban NPS pollution, especially raised the pollution load of urban runoff NPS in receiving water. Urban runoff pollution is a hot spot of research on NPS. This paper analyzed type,source and harm of the NPS pollutants of urban runoff and its influence on the receiving water. Through estimating NPS pollution load of urban runoff and summarizing the law and characteristics of urban runoff NPS systemically, study on management and control of urban runoff NPS pollution was focused on the application of BMPs (best management practices). It is a fresh methodology that management and control on NPS pollution from urban surface runoff was analyzed by methods of landscape ecology,environmental economics and environmental management. The paper provided a scientific reference for mitigating urban water environment pressure and an effective method for management and control of NPS pollution from urban surface runoff..

  9. Investigations of spherical Cu NPs in sodium lauryl sulphate with Tb{sup 3+} ions dispersed in PVA films

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Brijesh; Kaur, Gagandeep, E-mail: gagandeep_bhu@yahoo.com; Rai, S.B., E-mail: sbrai49@yahoo.co.in

    2016-03-15

    Highlights: • Cu NPs were prepared in SDS using 1064 nm laser radiation at fluence 37, 64 and 88 J/cm{sup 2}. • Spherical Cu NPs with average diameter varying between 10 and 50 nm atdifferent fluence. • PL of Tb3+ ions in PVA polymer film is maximum with Cu NPS at fluence 37 J/cm{sup 2}. • PVA films of Cu NPs displayed a highly temperature-dependent electrical conductivity. • These copper NPs embedded PVA films can be used as novel, low-cost sensor materials. - Abstract: Cu nanoparticles (NPs) have been prepared in SDS solution using 1064 nm laser radiation at different fluence 37 J/cm{sup 2}, 64 J/cm{sup 2} and 88 J/cm{sup 2} and structurally characterized. The TEM measurements reveal the presence of nanoparticles of spherical shape with different size. The size of the nanoparticles and their concentration increases with the increase of fluence.The effect of these Cu nanoparticles on the emissive properties of Tb{sup 3+} ion in polymer films has been studied. It is found that emission intensity of Tb{sup 3+} first increases and then deceases both with concentration of Cu NPs as well as with sizes. The PL intensity of Tb{sup 3+} ions is minimum for Cu NPs prepared with highest fluence. It has been explained in term of local field effect. This was also verified by life time measurements. These thin PVA films of copper nanoparticles displayed a highly temperature-dependent electrical conductivity with sensitivity at least comparable to commercial materials which suggest the use of these copper NPs embedded PVA films as novel, low-cost sensor materials.

  10. Impact of bio-palladium nanoparticles (bio-Pd NPs) on the activity and structure of a marine microbial community.

    Science.gov (United States)

    Nuzzo, Andrea; Hosseinkhani, Baharak; Boon, Nico; Zanaroli, Giulio; Fava, Fabio

    2017-01-01

    Biogenic palladium nanoparticles (bio-Pd NPs) represent a promising catalyst for organohalide remediation in water and sediments. However, the available information regarding their possible impact in case of release into the environment, particularly on the environmental microbiota, is limited. In this study the toxicity of bio-Pd NPs on the model marine bacterium V. fischeri was assessed. The impacts of different concentrations of bio-Pd NPs on the respiratory metabolisms (i.e. organohalide respiration, sulfate reduction and methanogenesis) and the structure of a PCB-dechlorinating microbial community enriched form a marine sediment were also investigated in microcosms mimicking the actual sampling site conditions. Bio-Pd NPs had no toxic effect on V. fischeri. In addition, they had no significant effects on PCB-dehalogenating activity, while showing a partial, dose-dependent inhibitory effect on sulfate reduction as well as on methanogenesis. No toxic effects by bio-Pd NPs could be also observed on the total bacterial community structure, as its biodiversity was increased compared to the not exposed community. In addition, resilience of the microbial community to bio-Pd NPs exposure was observed, being the final community organization (Gini coefficient) of samples exposed to bio-Pd NPs similar to that of the not exposed one. Considering all the factors evaluated, bio-Pd NPs could be deemed as non-toxic to the marine microbiota in the conditions tested. This is the first study in which the impact of bio-Pd NPs is extensively evaluated over a microbial community in relevant environmental conditions, providing important information for the assessment of their environmental safety.

  11. Influence of HCl on the NPs-CdSe synthesis prepared by the colloidal method

    Directory of Open Access Journals (Sweden)

    J. Sarmiento Arellano

    2016-08-01

    Full Text Available Cadmium selenide nanoparticles (NPs-CdSe were synthesized by colloidal route at room temperature and atmospheric pressure using cadmium chloride (CdCl2·2.5 hydrate and elemental selenium (Se as precursors. Sodium borohydride (NaBH4 was used as reducing agent to obtain Se2− ions and an aqueous solution with a NaOH and Penta sodium tripolyphosphate (STPP was used to protect Cd2+ ions. To remove the by-products generated during the chemical reaction and to promote the precipitation of NPs-CdSe, a cleaning process with an aqueous solution of HCl was performed. The HCl volume was varied from 0.2 to 1.2 ml during the cleaning process to study its effects on CdSe synthesis. The crystalline structure was analyzed by inspection of the high-resolution transmission electron microscope (HR-TEM and X-ray diffraction (XRD. This analysis showed that crystals of CdSe exhibit a face-centered cubic structure (FCC. The calculated crystallite size is 3.5 nm and increases to 4.5 nm as the HCl volume increases. The morphologies of the products were observed by SEM and TEM techniques. HRTEM images showed that NPs-CdSe synthetized to 0.8 ml are composed of a great number of homogeneous and smooth nanospheres which are not appreciable in SEM but are observable in TEM. By contrast, 0.2 and 1.2 ml HCl samples are comprised of a great deal of rods of compounds of Se mixed with CdSe spheres nanostructures. This work, which did not require the use of surfactants complexes or specials environment, is considered to have advantages over other works.

  12. Colorimetric detection of melamine based on p-chlorobenzenesulfonic acid-modified AuNPs

    Science.gov (United States)

    Li, Jianfang; Huang, Pengcheng; Wu, Fangying

    2016-06-01

    A highly selective and sensitive method is developed for colorimetric detection of melamine using gold nanoparticles (AuNPs) functionalized with p-chlorobenzenesulfonic acid. The addition of melamine induced the aggregation of AuNPs, as evidenced from the morphological characterizations and the color changed from red wine to blue, which could also be monitored by the UV-visible spectrometer and even naked eyes. This process caused a significant increase in the absorbance ratio (A650nm/A520nm) of p-chlorobenzenesulfonic acid-AuNPs. Under optimized conditions, the system exhibited a linear response to melamine in the range of 6.0 × 10-7-1.5 × 10-6 mol L-1 with a correlation coefficient of 0.997, and the limit of detection can even be 2.3 nM, which was much lower than some other methods and the safe limits (20 μM in both the USA and EU, 8.0 μM for infant formula in China, 1.2 μM in the CAC (Codex Alimentarius Commission) review for melamine in liquid infant formula). More importantly, the developed method presented excellent tolerance to coexisting common metal ions such as Ca2+, Zn2+, whose concentration is 1000 times of melamine, so that it had been applied to the analysis of melamine in liquid milk and milk powder with the recovery of 97.0-101 % and 100-103 %, respectively, indicating that the proposed method is quite a highly effective means to determine melamine in milk products.

  13. Navy-Wide Personnel Survey (NPS) 1992: Graphic Presentation of Results for Officers

    Science.gov (United States)

    1993-08-01

    CHILDRE N’S S EJADEOT TIME TO MOVING LENGTH OF NEW AW/T0oITH f- o 10 20 30 40 s0 0 70 0 90 100 Percent NPS-92 MULTIPLE RESPONSE QUESTION 014 REASON WHY...Sexual harassment is a form of sex " relationship.. which Is unduly familiar and discrimination that involves unwelcome - does not respect differences...harassment, both women M subordinate supervisory relationship exists. and men can be sexual harassers; people M can sexually harass persons of their own sex

  14. Connecting the dots: a collaborative USGS-NPS effort to expand the utility of monitoring data

    Science.gov (United States)

    Grace, James B.; Schoolmaster, Donald R.; Schweiger, E. William; Mitchell, Brian R.; Miller, Kathryn; Guntenspergen, Glenn R.

    2014-01-01

    The Natural Resource Challenge (National Park Service 1999) was a call to action. It constituted a mandate for monitoring based on the twin premises that (1) natural resources in national parks require active management and stewardship if we are to protect them from gradual degradation, and (2) we cannot protect what we do not understand. The intent of the challenge was embodied in its original description: We must expand existing inventory programs and develop efficient ways to monitor the vital signs of natural systems. We must enlist others in the scientific community to help, and also facilitate their inquiry. Managers must have and apply this information to preserve our natural resources. In this article, we report on ongoing collaborative work between the National Park Service (NPS) and the US Geological Survey (USGS) that seeks to add to our scientific understanding of the ecological processes operating behind vital signs monitoring data. The ultimate goal of this work is to provide insights that can facilitate an understanding of the systems and identify potential opportunities for active stewardship by NPS managers (Bennetts et al. 2007; Mitchell et al. 2014). The bulk of the work thus far has involved Acadia and Rocky Mountain national parks, but there are plans for extending the work to additional parks. Our story stats with work designed to consider ways of assessing the status and condition of natural resources and the potential for historical or ongoing influences of human activities. In the 1990s, the concept of "biotic integrity" began to take hold as an aspiration for developing quantitative indices describing how closely the conditions at a site resemble those found at pristine, unimpacted sites. Quantitative methods for developing indices of biotic integrity (IBIs) and elaborations of that idea (e.g., ecological integrity) have received considerable attention and application of these methods to natural resources has become widespread (Karr 1991

  15. Spectroscopic probe to contribution of physicochemical transformations in the toxicity of aged ZnO NPs to Chlorella vulgaris: new insight into the variation of toxicity of ZnO NPs under aging process.

    Science.gov (United States)

    Zhang, Hong; Huang, Qing; Xu, An; Wu, Lijun

    2016-10-01

    Zinc oxide nanoparticles (ZnO NPs) are one of the most abundantly applied nanomaterials in nanotechnology-based industries and they may cause unexpected environmental and health risks with their physicochemical transformations in the environment. Currently, there is still a lack of the in-depth understanding of the toxicity of aged ZnO NPs to aquatic organisms, particularly demanding quantitative analysis of the physicochemical transformations to distinguish their contributions in the toxicity assessment. For this purpose, therefore, we initiated the study of the toxicity of aged ZnO NPs to the model aquatic microalga, i.e. Chlorella vulgaris, and with the aid of spectroscopic tools for characterization and quantification of the physicochemical transformations, we scrutinized the toxicity variations for ZnO NPs with different aging times. As a result, we found that the toxicity altered in an abnormal manner with the aging time, i.e. the toxicity of aged ZnO NPs for 30 days showed the higher toxicity to the green alga than the fresh ZnO NPs or the ZnO NPs aged for longer time (e.g. 120 and 210 days). Through spectroscopic tools such as XRD, FTIR and Raman spectroscopy, we made both the qualitative and quantitative assessments of the physicochemical changes of the ZnO NPs, and confirmed that in the early stage, the toxicity mainly stemmed from the release of zinc ions, but with longer aging time, the neoformation of the nanoparticles played the critical role, leading to the overall reduced toxicity due to the less toxic hydrozincite and zinc hydroxide in the transformed compounds.

  16. The inhibitory effect of disulfiram encapsulated PLGA NPs on tumor growth: Different administration routes.

    Science.gov (United States)

    Fasehee, Hamidreza; Zarrinrad, Ghazaleh; Tavangar, Seyed Mohammad; Ghaffari, Seyed Hamidollah; Faghihi, Shahab

    2016-06-01

    The strong anticancer activity of disulfiram is hindered by its rapid degradation in blood system. A novel folate-receptor-targeted poly (lactide-co-glycolide) (PLGA)-polyethylene glycol (PEG) nanoparticle (NP) is developed for encapsulation and delivery of disulfiram into breast cancer tumor using passive (EPR effect) and active (folate receptor) targeting. The anticancer activity of disulfiram and its effect on caspase-3 activity and cell cycle are studied. The administration of encapsulated PLGA NPs using intra-peritoneal, intravenous and intra-tumor routes is investigated using animal model. Disulfiram shows strong cytotoxicity against MCF7 cell line. The activity of caspase-3 inhibited with disulfiram via dose dependent manner while the drug causes cell cycle arrest in G0/G1 and S phase time-dependently. The encapsulated disulfiram shows higher activity in apoptosis induction as compared to free drug. In nontoxic dose of encapsulated disulfiram, the highest and lowest efficacy of NPs in tumor growth inhibition is observed for intravenous injection and intraperitoneal injection. It is suggested that administration of disulfiram by targeted PLGA nanoparticles using intravenous injection would present an alternative therapeutic approach for solid tumor treatment.

  17. Surface-Renewable AgNPs/CNT/rGO Nanocomposites as Bifunctional Impedimetric Sensors

    Institute of Scientific and Technical Information of China (English)

    Azadeh Azadbakht; Amir Reza Abbasi; Zohreh Derikvand; Ziba Karimi; Mahmoud Roushani

    2017-01-01

    In this study, glassy carbon electrode modified by silver nanoparticles/carbon nanotube/reduced graphene oxide (AgNPs/CNT/rGO) composite has been utilized as a platform to immobilize cis-dioxomolybdenum (VI)–salicylaldehyde-histidine (MoO2/Sal-His). The modified electrode shows two reversible redox couples for MoO2/Sal-His. Electrocatalytic oxidation of cysteine (CySH) and electrocatalytic reduction of iodate on the surface of the modified electrode were investigated with cyclic voltammetry and electrochemical impedance spectroscopy methods. The presence of MoO2/Sal-His on AgNPs/CNT/rGO shifted the catalytic current of iodate reduction to a more positive potential and the catalytic current of cysteine oxidation to a more negative potential. The change of interfacial charge transfer resistance (Rct) recorded by the modified electrode was monitored for sensitive quantitative detection of CySH and iodate. Moreover, the sensor has a good stability, and it can be renewed easily and repeatedly through a mechanical or electrochemical process.

  18. Enhance antimicrobial activity of ZnO nanomaterial's (QDs and NPs) and their analytical applications

    Science.gov (United States)

    Wahab, Rizwan; Khan, Farheen; Lutfullah; Singh, R. B.; Khan, Asif

    2014-08-01

    The small scale nanomaterial's such as quantum dots and nanoparticles, which have unique properties studied under varying conditions. Various techniques were used in order to best understand the structure of ZnO nanoparticles and quantum dots, which inhibit the growth of Escherichia coli bacteria under dose dependent manner. The results of obtained datas were confirmed with statistical analytical methods. The linear regressive equation data obtained from UV-visible spectra, which give satisfactory results and allows to quickly and easily calculate statistical datas such as: concentration range of analytes, mean, standard deviation, variance and standard analytical error, relative standard deviation, coefficient of correlation, variance of regression line errors in the slope and the intercept, confidence limit for the slope and the intercept. Instead, validation is an important trait measure by any methods due to near to the quality of results. The proposed method validated by specificity, precision, accuracy, limit detection and quantitation. The absorption spectra of sample solution were recorded maximum absorbance for both nanoparticles (NPs) and quantum dots (QDs) at ʎmax 600 nm, this wavelength was suitable and selected for all operational analytical measurements. The size and shape of the prepared NPs and QDs were examined with sophisticated instruments such as FESEM and TEM, whereas crystallinity of the prepared materials was analyzed with X-ray diffraction pattern (XRD).

  19. NPS: A Tested Platform for Political Transformation Against Anger & Apathy in Pakistan

    Directory of Open Access Journals (Sweden)

    Allah Nawaz

    2013-07-01

    Full Text Available Political inaction is a major ‘Barrier’ to the progress of democratic values and systems in a society. It is more critical in the developing countries like Pakistan. A huge body of research on political apathy and anger in Pakistan is reporting over and over on the causes and consequences of this pathetic psychology. All that is true however, rays of hope are always there provided nations continue searching for the opportunities through scientific and rigorous research accompanied with sincerity and sense of responsibility at all the decision making levels of the state. This paper postulates a solution model for the issue in the perspectives of Pakistan by capitalizing on the role of Information and Communication Technologies (ICT in the Instant Political Transformation of the developing countries like Egypt & Lybia. The New Public Sphere (NPS is populated with Global Civil Society (GCS where International Citizens are connected together 24/7 from any corner of the Global Village and involved in use of ICT for Social Activism. Pakistan now has millions of Internet and Cell-users who are the part of GCS and waiting for a ‘Trigger’ to switch from the ‘Informal Activism to Formal & Political Activism’ through NPS.

  20. The utilization of SiNWs/AuNPs-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor

    Energy Technology Data Exchange (ETDEWEB)

    Rashid, Jahwarhar Izuan Abdul [Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Department of Chemistry and Biology, Centre for Defense Foundation Studies, National Defense University of Malaysia, Sungai Besi Camp, 57000 Kuala Lumpur (Malaysia); Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Yusof, Nor Azah, E-mail: azahy@upm.edu.my [Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Abdullah, Jaafar [Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Hashim, Uda [Institute of Nanoelectronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis (Malaysia); Hajian, Reza, E-mail: rezahajian@upm.edu.my [Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia)

    2014-12-01

    This work describes the incorporation of SiNWs/AuNPs composite as a sensing material for DNA detection on indium tin-oxide (ITO) coated glass slide. The morphology of SiNWs/AuNPs composite as the modifier layer on ITO was studied by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The morphological studies clearly showed that SiNWs were successfully decorated with 20 nm-AuNPs using self-assembly monolayer (SAM) technique. The effective surface area for SiNWs/AuNPs-modified ITO enhanced about 10 times compared with bare ITO electrode. SiNWs/AuNPs nanocomposite was further explored as a matrix for DNA probe immobilization in detection of dengue virus as a bio-sensing model to evaluate its performance in electrochemical sensors. The hybridization of complementary DNA was monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as the redox indicator. The fabricated biosensor was able to discriminate significantly complementary, non-complementary and single-base mismatch oligonucleotides. The electrochemical biosensor was sensitive to target DNA related to dengue virus in the range of 9.0–178.0 ng/ml with detection limit of 3.5 ng/ml. In addition, SiNWs/AuNPs-modified ITO, regenerated up to 8 times and its stability was up to 10 weeks at 4 °C in silica gel. - Highlights: • A sensitive biosensor is presented for detection of dengue virus. • SiNWs and AuNPs used as nanocomposite layers on ITO for construction of biosensor • The detection mechanism is based on the interaction of MB with DNA bonded on AuNPs. • The reduction signal of MB decreases upon complementary hybridization.

  1. Development of a LIDAR for integration with the Naval Postgraduate School Infrared Search and Target Designation (NPS-IRSTD) system

    OpenAIRE

    Regush, Murray Michael

    1993-01-01

    Approved for public release; distribution is unlimited. A lidar was designed and manufactured at the Naval Postgraduate School, Monterey, CA, to provide range information to atmospheric features, such as clouds. It is further planned to integrate the lidar with the NPS-IRSTD system at some future date. The NPS-IRSTD uses two vertical linear focal plane arrays for target detection and target direction can be determined very accurately but the system does not provide any useful range informa...

  2. A Whole City Response to the Use of Novel Psychoactive Substances (NPS) by Young People in Newcastle-upon-Tyne

    OpenAIRE

    2015-01-01

    In recent years, Novel Psychoactive Substances (NPS) (otherwise known as ‘legal highs’) have posed an increasingly significant problem for practitioners and policy makers due to their widespread availability, largely unknown chemical composition and negative effects on health and wellbeing. Young people (aged 10-25) seem to be at particular risk of using NPS and experiencing the associated harms. As such, the Department of Social Sciences and Languages at Northumbria University were commissio...

  3. The utilization of SiNWs/AuNPs-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor.

    Science.gov (United States)

    Rashid, Jahwarhar Izuan Abdul; Yusof, Nor Azah; Abdullah, Jaafar; Hashim, Uda; Hajian, Reza

    2014-12-01

    This work describes the incorporation of SiNWs/AuNPs composite as a sensing material for DNA detection on indium tin-oxide (ITO) coated glass slide. The morphology of SiNWs/AuNPs composite as the modifier layer on ITO was studied by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The morphological studies clearly showed that SiNWs were successfully decorated with 20 nm-AuNPs using self-assembly monolayer (SAM) technique. The effective surface area for SiNWs/AuNPs-modified ITO enhanced about 10 times compared with bare ITO electrode. SiNWs/AuNPs nanocomposite was further explored as a matrix for DNA probe immobilization in detection of dengue virus as a bio-sensing model to evaluate its performance in electrochemical sensors. The hybridization of complementary DNA was monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as the redox indicator. The fabricated biosensor was able to discriminate significantly complementary, non-complementary and single-base mismatch oligonucleotides. The electrochemical biosensor was sensitive to target DNA related to dengue virus in the range of 9.0-178.0 ng/ml with detection limit of 3.5 ng/ml. In addition, SiNWs/AuNPs-modified ITO, regenerated up to 8 times and its stability was up to 10 weeks at 4°C in silica gel.

  4. Calcium-sensing receptor antagonist NPS2390 attenuates neuronal apoptosis though intrinsic pathway following traumatic brain injury in rats.

    Science.gov (United States)

    Xue, Zhaoliang; Song, Zhengfei; Wan, Yingfeng; Wang, Kun; Mo, Lianjie; Wang, Yirong

    2017-04-29

    Traumatic brain injury (TBI) initiates a complex cascade of neurochemical and signaling changes that leads to neuronal apoptosis, which contributes to poor outcomes for patients with TBI. Previous study indicates that calcium-sensing receptor (CaSR) activation contributes to neuron death in focal cerebral ischemia-reperfusion mice, however, its role in neuronal apoptosis after TBI is not well-established. Using a controlled cortical impact model in rats, the present study was designed to determine the effect of CaSR inhibitor NPS2390 upon neuronal apoptosis after TBI. Rats were randomly distributed into three groups undergoing the sham surgery or TBI procedure, and NPS2390 (1.5 mg/kg) was infused subcutaneously at 30 min and 120 min after TBI. All rats were sacrificed at 24 h after TBI. Our data indicated that NPS2390 significantly reduced the brain edema and improved the neurological function after TBI. In addition, NPS2390 decreased caspase-3 levels and the number of apoptotic neurons. Furthermore, NPS2390 up-regulated anti-apoptotic protein Bcl-2 expression and down-regulated pro-apoptotic protein Bax, and reduced subsequent release of cytochrome c into the cytosol. In summary, this study indicated that inhibition of CaSR by NPS2390 attenuates neuronal apoptosis after TBI, in part, through modulating intrinsic apoptotic pathway. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Facile biosynthesis of Ag-NPs using Otostegia limbata plant extract: Physical characterization and auspicious biological activities

    Science.gov (United States)

    Kausar, Rizwan; Shaheen, Muhammad Ashraf; Maqbool, Qaisar; Naz, Sania; Nazar, Mudassar; Abbas, Fazal; Hussain, Talib; Younas, Umer; Shams, Muhammad Fahad

    2016-09-01

    Silver nanoparticles (Ag-NPs) synthesized through reduction by Otostegia limbata green extract are, hereby, reported for the first time. It is very interesting to observe that in this case, O. limbata plant extract acts as a strong chelating agent in Ag-NPs formation through AgNO3. Scanning electron microscope (SEM) studies expose that Ag-NPs formation is highly homogenous and spherical with mean particle size of 32 ±0.8 nm. A typical Ag absorption peak has been observed at 419 nm by ultra violet (UV)-visible spectroscopy which have endorsed the successful formation of single phase Ag-NPs. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) examination further validates the crystalline pure phase structure of Ag-NPs. Promising results have been recorded against protein kinase inhibition assay and antibacterial assay having prominent pathogenic strains. Our present study explores that biosynthesized eco-friendly Ag-NPs have great potential, in the future, for anticancer drug development with wide range pharmaceutical applications.

  6. Effects of computing parameters and measurement locations on the estimation of 3D NPS in non-stationary MDCT images.

    Science.gov (United States)

    Miéville, Frédéric A; Bolard, Gregory; Bulling, Shelley; Gudinchet, François; Bochud, François O; Verdun, François R

    2013-11-01

    The goal of this study was to investigate the impact of computing parameters and the location of volumes of interest (VOI) on the calculation of 3D noise power spectrum (NPS) in order to determine an optimal set of computing parameters and propose a robust method for evaluating the noise properties of imaging systems. Noise stationarity in noise volumes acquired with a water phantom on a 128-MDCT and a 320-MDCT scanner were analyzed in the spatial domain in order to define locally stationary VOIs. The influence of the computing parameters in the 3D NPS measurement: the sampling distances bx,y,z and the VOI lengths Lx,y,z, the number of VOIs NVOI and the structured noise were investigated to minimize measurement errors. The effect of the VOI locations on the NPS was also investigated. Results showed that the noise (standard deviation) varies more in the r-direction (phantom radius) than z-direction plane. A 25 × 25 × 40 mm(3) VOI associated with DFOV = 200 mm (Lx,y,z = 64, bx,y = 0.391 mm with 512 × 512 matrix) and a first-order detrending method to reduce structured noise led to an accurate NPS estimation. NPS estimated from off centered small VOIs had a directional dependency contrary to NPS obtained from large VOIs located in the center of the volume or from small VOIs located on a concentric circle. This showed that the VOI size and location play a major role in the determination of NPS when images are not stationary. This study emphasizes the need for consistent measurement methods to assess and compare image quality in CT.

  7. Specimens: "most of" generic NPs in a contextually flexible type theory

    CERN Document Server

    Retoré, Christian

    2011-01-01

    This paper proposes to compute the meanings associated to sentences with generic NPs corresponding to the most of generalized quantifier. We call these generics specimens and they resemble stereotypes or prototypes in lexical semantics. The meanings are viewed as logical formulae that can be thereafter interpreted in your favorite models. We rather depart from the dominant Fregean single untyped universe and go for type theory with hints from Hilbert epsilon calculus and from medieval philosophy. Our type theoretic analysis bears some resemblance with on going work in lexical semantics. Our model also applies to classical examples involving a class (or a generic element of this class) which is provided by the context. An outcome of this study is that, in the minimalism-contextualism debate, if one adopts a type theoretical view, terms encode the purely semantic meaning component while their typing is pragmatically determined.

  8. Rapid detection of NBOME's and other NPS on blotter papers by direct ATR-FTIR spectrometry.

    Science.gov (United States)

    Coelho Neto, José

    2015-07-01

    Blotter paper is among the most common forms of consumption of new psychotropic substances (NPS), formerly referred as designer drugs. In many cases, users are misled to believe they are taking LSD when, in fact, they are taking newer and less known drugs like the NBOMEs or other substituted phenethylamines. We report our findings in quick testing of blotter papers for illicit substances like NBOMEs and other NPS by taking ATR-FTIR spectra directly from blotters seized on the streets, without any sample preparation. Both sides (front and back) of each blotter were tested. Collected data were analyzed by single- and multi-component spectral matching and submitted to chemometric discriminant analysis. Our results showed that, on 66.7% of the cases analyzed, seized blotters contained one or more types of NBOMEs, confirming the growing presence of this novel substances on the market. Matching IR signals were detected on both or just one side of the blotters and showed variable strength. Although no quantitative analysis was made, detection of these substances by the proposed approach serves as indication of variable and possibly higher dosages per blotter when compared to LSD, which showed to be below the detection limit of the applied method. Blotters containing a mescaline-like compound, later confirmed by GC-MS and LC-MS to be MAL (methallylescaline), a substance very similar to mescaline, were detected among the samples tested. Validity of direct ATR-FTIR testing was confirmed by checking the obtained results against independent GC-MS or LC-MS results for the same cases/samples.

  9. Estimation on the Soil Erosion Type Non-point Pollution based on the DEM%基于 DEM 的水土流失型非点源污染估算

    Institute of Scientific and Technical Information of China (English)

    陈邦雄

    2014-01-01

    Soil erosion is not only the non -point pollution form but also the carrier of other non -point pollutant los-ses.Taking the Daya Bay watershed as the example , the basic materials were gained by using the Digital Elevation Model( DEM) .Based on the analogy of the related research findings at home and abroad , it was discussed the rapid appraisal method to the large scale soil erosion type non -point pollution load to get of the parameters of export coeffi-cient model for the estimation of the soil erosion type non -point pollution load .%水土流失既是一种非点源污染形式,同时又是其他非点源污染物流失的载体。本文以大亚湾流域为例,利用数字高程模型( DEM)获取基础资料,通过类比国内外的相关研究成果而获得的输出系数模型参数,采用输出系数模型法估算水土流失型非点源污染负荷,探讨快速评估大尺度水土流失型非点源污染负荷的方法。

  10. Neuropharmacology of new psychoactive substances (NPS: focus on the rewarding and reinforcing properties of cannabimimetics and amphetamine-like stimulants

    Directory of Open Access Journals (Sweden)

    Cristina eMiliano

    2016-04-01

    Full Text Available New psychoactive substances (NPS are a heterogeneous and rapidly evolving class of molecules available on the global illicit drug market (e.g smart shops, internet, dark net as a substitute for controlled substances. The use of NPS, mainly consumed along with other drugs of abuse and/or alcohol, has resulted in a significantly growing number of mortality and emergency admissions for overdoses, as reported by several poison centers from all over the world. The fact that the number of NPS have more than doubled over the last 10 years, is a critical challenge to governments, the scientific community, and civil society (UNODC, World Drug Report, 2014; EMCDDA, European Drug Report 2014: Trends and developments. The chemical structure (phenethylamines, piperazine, cathinones, tryptamines, synthetic cannabinoids of NPS and their pharmacological and clinical effects (hallucinogenic, anesthetic, dissociative, depressant help classify them into different categories. In the recent past, 50% of newly identified NPS have been classified as synthetic cannabinoids followed by new phenethylamines (17%(WDR, 2014. Besides peripheral toxicological effects, many NPS seem to have addictive properties. Behavioral, neurochemical, and electrophysiological evidence can help in detecting them. This manuscript will review existing literature about the addictive and rewarding properties of the most popular NPS classes: cannabimimetics (JWH, HU, CP series and amphetamine-like stimulants (amphetamine, methamphetamine, methcathinone and MDMA analogues. Moreover, the review will include recent data from our lab which links JWH-018, a CB1 and CB2 agonist more potent than Δ9-THC, to other cannabinoids with known abuse potential, and to other classes of abused drugs that increase dopamine signaling in the Nucleus Accumbens (NAc shell. Thus the neurochemical mechanisms that produce the rewarding properties of JWH-018, which most likely contributes to the greater incidence of

  11. Neuropharmacology of New Psychoactive Substances (NPS): Focus on the Rewarding and Reinforcing Properties of Cannabimimetics and Amphetamine-Like Stimulants.

    Science.gov (United States)

    Miliano, Cristina; Serpelloni, Giovanni; Rimondo, Claudia; Mereu, Maddalena; Marti, Matteo; De Luca, Maria Antonietta

    2016-01-01

    New psychoactive substances (NPS) are a heterogeneous and rapidly evolving class of molecules available on the global illicit drug market (e.g smart shops, internet, "dark net") as a substitute for controlled substances. The use of NPS, mainly consumed along with other drugs of abuse and/or alcohol, has resulted in a significantly growing number of mortality and emergency admissions for overdoses, as reported by several poison centers from all over the world. The fact that the number of NPS have more than doubled over the last 10 years, is a critical challenge to governments, the scientific community, and civil society [EMCDDA (European Drug Report), 2014; UNODC, 2014b; Trends and developments]. The chemical structure (phenethylamines, piperazines, cathinones, tryptamines, synthetic cannabinoids) of NPS and their pharmacological and clinical effects (hallucinogenic, anesthetic, dissociative, depressant) help classify them into different categories. In the recent past, 50% of newly identified NPS have been classified as synthetic cannabinoids followed by new phenethylamines (17%) (UNODC, 2014b). Besides peripheral toxicological effects, many NPS seem to have addictive properties. Behavioral, neurochemical, and electrophysiological evidence can help in detecting them. This manuscript will review existing literature about the addictive and rewarding properties of the most popular NPS classes: cannabimimetics (JWH, HU, CP series) and amphetamine-like stimulants (amphetamine, methamphetamine, methcathinone, and MDMA analogs). Moreover, the review will include recent data from our lab which links JWH-018, a CB1 and CB2 agonist more potent than Δ(9)-THC, to other cannabinoids with known abuse potential, and to other classes of abused drugs that increase dopamine signaling in the Nucleus Accumbens (NAc) shell. Thus the neurochemical mechanisms that produce the rewarding properties of JWH-018, which most likely contributes to the greater incidence of dependence associated

  12. Green synthesis of ZnO NPs from various parts of Azhadirachta indica (neem) plant as biotemplates for anti-bacterial applications

    Science.gov (United States)

    Sharma, Savita; Oudhia, Anjali

    2016-05-01

    A green and cost effective method of production of ZnO nanoparticles (NPs) was developed using extracts from different parts of Neem plant (e.g. leaf, seed, and stem) as bio templates. ZnO NPs obtained through these bio-templates exhibit different structures, morphologies and optical properties, showing potential of the present method in preparing NPs with desired properties, FTIR and XRD techniques were used for characterization. A comparison of antibacterial activities of ZnO NPs show decreasing zone of inhibition with increasing particle sizes. The as prepared ZnO NPs functionalized with various materials to make them bio compatible. It was observed that natural tri-peptide GSH was most effective capping agents for controlling the toxicity of ZnO NPs. A mechanism is proposed to explain the variation in growth patterns and properties of ZnO NPs obtained from the aforementioned bio-templates.

  13. High-performance SERS substrate based on hybrid structure of graphene oxide/AgNPs/Cu film@pyramid Si

    Science.gov (United States)

    Li, Zhe; Xu, Shi Cai; Zhang, Chao; Liu, Xiao Yun; Gao, Sai Sai; Hu, Li Tao; Guo, Jia; Ma, Yong; Jiang, Shou Zhen; Si, Hai Peng

    2016-12-01

    We present a novel surface-enhanced Raman scattering (SERS) substrate based on graphene oxide/silver nanoparticles/copper film covered silicon pyramid arrays (GO/AgNPs/PCu@Si) by a low-cost and simple method. The GO/AgNPs/PCu@Si substrate presents high sensitivity, good homogeneity and well stability with R6G molecules as a probe. The detected concentration of Rhodamine 6 G (R6G) is as low as 10-15 M. These sensitive SERS behaviors are also confirmed in theory via a commercial COMSOL software, the electric field enhancement is not only formed between the AgNPs, but also formed between the AgNPs and Cu film. And the GO/AgNPs/PCu@Si substrates also present good property on practical application for the detection of methylene blue (MB) and crystal violet (CV). This work may offer a novel and practical method to facilitate the SERS applications in areas of medicine, food safety and biotechnology.

  14. Laminin Receptor-Avid Nanotherapeutic EGCg-AuNPs as a Potential Alternative Therapeutic Approach to Prevent Restenosis

    Directory of Open Access Journals (Sweden)

    Menka Khoobchandani

    2016-03-01

    Full Text Available In our efforts to develop new approaches to treat and prevent human vascular diseases, we report herein our results on the proliferation and migration of human smooth muscles cells (SMCs and endothelial cells (ECs using epigallocatechin-3-gallate conjugated gold nanoparticles (EGCg-AuNPs as possible alternatives to drug coated stents. Detailed in vitro stability studies of EGCg-AuNPs in various biological fluids, affinity and selectivity towards SMCs and ECs have been investigated. The EGCg-AuNPs showed selective inhibitory efficacy toward the migration of SMCs. However, the endothelial cells remained unaffected under similar experimental conditions. The cellular internalization studies have indicated that EGCg-AuNPs internalize into the SMCs and ECs within short periods of time through laminin receptor mediated endocytosis mode. Favorable toxicity profiles and selective affinity toward SMCs and ECs suggest that EGCg-AuNPs may provide attractive alternatives to drug coated stents and therefore offer new therapeutic approaches in treating cardiovascular diseases.

  15. A ratiometric nanosensor based on conjugated polyelectrolyte-stabilized AgNPs for ultrasensitive fluorescent and colorimetric sensing of melamine.

    Science.gov (United States)

    Zhu, Xixi; Xiao, Yi; Jiang, Xiaoying; Li, Jiahui; Qin, Hongling; Huang, Hongmei; Zhang, Youyu; He, Xiaoxiao; Wang, Kemin

    2016-05-01

    A new ratiometric nanosensor is developed for selective and ultrasensitive detection of melamine based on conjugated polyelectrolyte (CPE)-stabilized silver nanoparticles (P1-AgNPs) by perfectly combining the advantages of CPE and AgNPs. P1 featuring a π-delocalized backbone bearing pyridinyl groups can act as an excellent dual-emission fluorescent probe as well as a polymer localizer for AgNPs. In the presence of melamine, the fluorescence intensity at 386nm increases owing to the turn-on of the fluorescence of P1, whereas FL intensity at 488nm decreases due to the melamine-induced aggregation and subsequent aggregation-enhanced emission quenching of P1-AgNPs, therefore leading to the ratiometric fluorescent sensing of analyte. Moreover, analyte-induced aggregation of P1-AgNPs also allows the ratiometric colorimetric measurement of melamine. Under the optimum conditions, this facile ratiometric nanosensor favors the fluorescent and colorimetric determination of melamine in liquid milk products with the detection limit as low as 0.1 and 0.45nM, respectively.

  16. Electrochemical Characterization of Graphene and MWCNT Screen-Printed Electrodes Modified with AuNPs for Laccase Biosensor Development

    Directory of Open Access Journals (Sweden)

    Gabriele Favero

    2015-11-01

    Full Text Available The aim of this work is to show how the integration of gold nanoparticles (AuNPs into multi-wall-carbon-nanotubes (MWCNTs based screen-printed electrodes and into graphene-based screen-printed electrodes (GPHs could represent a potential way to further enhance the electrochemical properties of those electrodes based on nanoparticles. Laccase from Trametes versicolor (TvL was immobilized over MWCNTs and GPH previously modified with AuNPs (of 5 and 10 nm. The characterization of the modified electrode surface has been carried out by cyclic voltammetry. The results showed that the use of AuNPs for modification of both graphene and MWCNTs screen-printed electrode surfaces would increase the electrochemical performances of the electrodes. MWCNTs showed better results than GPH in terms of higher electroactive area formation after modification with AuNPs. The two modified nanostructured electrodes were successively proven to efficiently immobilize the TvL; the electrochemical sensing properties of the GPH- and MWCNT-based AuNPs-TvL biosensors were investigated by choosing 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid diammonium salt (ABTS, catechol and caffeic acid as laccase mediators; and the kinetic parameters of the laccase biosensor were carefully evaluated.

  17. High-performance SERS substrate based on hybrid structure of graphene oxide/AgNPs/Cu film@pyramid Si.

    Science.gov (United States)

    Li, Zhe; Xu, Shi Cai; Zhang, Chao; Liu, Xiao Yun; Gao, Sai Sai; Hu, Li Tao; Guo, Jia; Ma, Yong; Jiang, Shou Zhen; Si, Hai Peng

    2016-12-07

    We present a novel surface-enhanced Raman scattering (SERS) substrate based on graphene oxide/silver nanoparticles/copper film covered silicon pyramid arrays (GO/AgNPs/PCu@Si) by a low-cost and simple method. The GO/AgNPs/PCu@Si substrate presents high sensitivity, good homogeneity and well stability with R6G molecules as a probe. The detected concentration of Rhodamine 6 G (R6G) is as low as 10(-15) M. These sensitive SERS behaviors are also confirmed in theory via a commercial COMSOL software, the electric field enhancement is not only formed between the AgNPs, but also formed between the AgNPs and Cu film. And the GO/AgNPs/PCu@Si substrates also present good property on practical application for the detection of methylene blue (MB) and crystal violet (CV). This work may offer a novel and practical method to facilitate the SERS applications in areas of medicine, food safety and biotechnology.

  18. Preparation of reduced graphene oxide/meso-TiO{sub 2}/AuNPs ternary composites and their visible-light-induced photocatalytic degradation n of methylene blue

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Yongfang; Ma, Zheng; Xu, Lidong [School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130 (China); Wang, Hefang, E-mail: whf0618@163.com [School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130 (China); Fu, Nian, E-mail: funian3678@163.com [School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130 (China); College of Physics Science and Technology of Hebei University, Baoding 071002 (China)

    2016-04-30

    Graphical abstract: Reduced graphene oxide/meso-TiO{sub 2}/AuNPs (RGO/meso-TiO{sub 2}/AuNPs) ternary composites were prepared via the addition of graphene oxide to the dispersion of meso-TiO{sub 2}/AuNPs under a hydrothermal condition. The RGO/meso-TiO{sub 2}/AuNPs ternary composites show high photocatalytic activity toward MB. - Highlights: • RGO/meso-TiO{sub 2}/AuNPs were obtained by addition of graphene oxide to meso-TiO{sub 2}/AuNPs. • Au NPs in the mesopores of meso-TiO{sub 2} reduce the recombination of charge carriers. • RGO covered with the surface of the meso-TiO{sub 2} enhance the adsorption of MB. • RGO/meso-TiO{sub 2}/AuNPs composites show high photocatalytic performance toward MB. - Abstract: Reduced graphene oxide/meso-TiO{sub 2}/AuNPs (RGO/meso-TiO{sub 2}/AuNPs) ternary composites were prepared via the addition of graphene oxide to the dispersion of meso-TiO{sub 2}/AuNPs under hydrothermal conditions. The structure and the morphology of the RGO/meso-TiO{sub 2}/AuNPs materials were characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The photocatalytic activity of RGO/meso-TiO{sub 2}/AuNPs was evaluated by degradation of methyl blue (MB) under visible-light illumination. The ternary composites present an extended light absorption range, efficient charge separation properties, high adsorption ability for MB and high photocatalytic degradation activity of MB compared to the meso-TiO{sub 2} and meso-TiO{sub 2}/AuNPs.

  19. Antibacterial and antifouling activities of chitosan/TiO2/Ag NPs nanocomposite films against packaged drinking water bacterial isolates.

    Science.gov (United States)

    Natarajan, Saravanan; Bhuvaneshwari, M; Lakshmi, D Shanthana; Mrudula, P; Chandrasekaran, N; Mukherjee, Amitava

    2016-10-01

    TiO2 and Ag NPs are widely used as antibacterial agents against many bacterial pathogens. Chitosan (polymer) itself acts as a strong antibacterial agent. Hence, chitosan/TiO2/Ag NPs incorporated nanocomposite film was prepared against packed drinking water bacterial strains. A concentration-dependent increase in the reduction of cell viability was observed in all the isolates under UV-C and dark exposure conditions. The bacteria consortium showed greater resistance against antibacterial effects of chitosan/TiO2/Ag nanocomposite as compared to single isolates. Glycocalyx test and mass assessment conclude the effective antibacterial activity by inhibiting bacterial adhesion on the film surface. The release of LDH and generation of ROS act as the predominant antibacterial mechanism induced by TiO2/Ag NPs. Surface characterization of chitosan/TiO2/Ag nanocomposite was studied by FTIR and XRD analyses and SEM analysis after interaction with the bacteria.

  20. Gold nanoparticles supported on mesoporous silica: origin of high activity and role of Au NPs in selective oxidation of cyclohexane

    Science.gov (United States)

    Wu, Pingping; Bai, Peng; Yan, Zifeng; Zhao, George X. S.

    2016-01-01

    Homogeneous immobilization of gold nanoparticles (Au NPs) on mesoporous silica has been achieved by using a one-pot synthesis method in the presence of organosilane mercapto-propyl-trimethoxysilane (MPTMS). The resultant Au NPs exhibited an excellent catalytic activity in the solvent-free selective oxidation of cyclohexane using molecular oxygen. By establishing the structure-performance relationship, the origin of the high activity of mesoporous supported Au catalyst was identified to be due to the presence of low-coordinated Au (0) sites with high dispersion. Au NPs were confirmed to play a critical role in the catalytic oxidation of cyclohexane by promoting the activation of O2 molecules and accelerating the formation of surface-active oxygen species.

  1. An Analysis of the Non-point Pollution Load on the Urban Roof in Xinxiang City%新乡市城区屋面非点源污染负荷分析

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    The purpose of this study is probing into the pollutants variation characteristics of urban roof runoff ,the quantification of the pollution load ,as well as analyzing the effect on the receiving water .The aim is to provide a basis for using urban rainfall effec‐tively ,controlling non-point source pollution ,improving water environment ,and so on .Taking urban roof as the catchments area in Xinxiang City ,we do research on the hydrology and water quality process of each rainfall event with natural rain condition .The sam‐ples were chosen to analyze the contents of SS ,COD and TN .The results show that the volume of urban roof runoff is 14 .786 4 mil‐lion m3 in 2012 .Meanwhile ,based on the event mean concentration as the main parameter ,the pollution load of SS ,COD and TN are 982 .96 t ,2 871 .62 t and 225 .71 t respectively ,and the proportions which discharges into the receiving water are 31 .7% , 15 .4% and 8 .5% of the total pollution load .%对城区屋面径流污染变化特征以及污染负荷的定量化研究,并分析其对受纳水体的影响,可为城市利用雨水、非点源污染控制、水环境改善等提供依据。以屋面作为汇水区域,开展天然降雨情况下场次降雨径流水文水质过程试验监测,水质指标包括SS、COD、T N。研究结果表明:2012年新乡市城区屋面径流量为1478.64万m3;以场次平均浓度作为主要参数,计算SS、COD、T N污染负荷量分别为982.96 t、2871.62 t、225.71 t ,分别占排入受纳水体总污染负荷的31.7%、15.4%、8.5%。

  2. The effect of CuO NPs on reactive oxygen species and cell cycle gene expression in roots of rice.

    Science.gov (United States)

    Wang, Shuling; Liu, Hanzhu; Zhang, Yuxi; Xin, Hua

    2015-03-01

    To evaluate the effect of CuO nanoparticles (NPs) on root growth, root reactive oxygen species (ROS) production, and the expression of 2 genes (OsCDC2 and OsCYCD) associated with root growth of Oryza sativa (rice), rice roots were treated with 5 mg/L CuO NP suspension, 5 mg/L CuO bulk particle suspension, and 0.27 mg/L CuSO4  · 5H2 O solution, with distilled water as control. The results indicated that CuO NPs and Cu(2+) severely inhibited the elongation and biomass of rice roots after 72-h exposure. Dyeing with 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) showed that in all 3 treatment groups, the fluorescence was primarily located in the meristem zone, demonstrating that the meristem zone was where ROS were primarily generated. In addition, a significant increase in ROS was detected in the meristem zone of roots treated with the CuO NP suspension and the CuSO4  · 5H2 O solution, both of which greatly influenced the expression level of OsCDC2 and OsCYCD. The impact of Cu(2+) on these 2 genes was smaller than that of CuO NPs. The Cu content in roots of rice after treatment with CuO NPs was much higher than that found after the other treatments, which indicated that CuO NPs may have been absorbed into root tissue. Collectively, these data suggest that growth inhibition, higher ROS production, and gene expression inhibition may be caused not only by the ions themselves, but also the NPs. © 2014 SETAC.

  3. Optimization of ZnO-NPs to Investigate Their Safe Application by Assessing Their Effect on Soil Nematode Caenorhabditis elegans.

    Science.gov (United States)

    Gupta, Shruti; Kushwah, Tanuja; Vishwakarma, Ashutosh; Yadav, Shweta

    2015-12-01

    Zinc oxide nanoparticles (ZnO-NPs) are increasingly receiving attention due to their widespread application in cosmetics, pigments and coatings. This has raised concerns in the public and scientific communities regarding their unexpected health effects. Toxicity effect of ZnO-NPs on the environment was assessed in the present study using Caenorhabditis elegans. Multiple toxicity end points including their mortality, behaviour, reproduction, in vitro distribution and expression of stress response mtl-1 and sod-1 genes were observed to evaluate safe application of ZnO-NPs. C. elegans were exposed to 10, 50, and 100 nm ZnO-NPs (0.1 to 2.0 g/l). Application of 10 nm ≥0.7g/l adversely affects the survivability of worms and was significantly not affected with exposure of 50 and 100 nm ≤1.0 g/l. However, reproduction was affected at much low concentration as compared to their survivability. LC50 was recorded 1.0 ± 0.06 (g/l) for 100 nm, 0.90 ± 0.60 for 50 nm and 0.620 ± 0.08 for 10 nm. Expression of mtl-1 and sod-1 was significantly increased with application of 10 nm ≥0.7g/l and significantly unaffected with exposure of 50 and 100 nm at the same concentration. ZnO-NPs (10 nm) had shown even distribution extended nearly the entire length of the body. The distribution pattern of ZnO-NPs indicates that the intestine is the major target tissues for NP toxicity. Study demonstrates that small-sized (10 nm) ZnO-NPs ≥0.7g/l is more toxic than larger-sized particles. This may be suggested on the basis of available data; application of 50 and 100 nm ≤1.0 g/l ZnO-NPs may be used to the environment as this shows no significant toxicity. However, further calibration is warranted to explore safe dose on soil compartments prior to their field application.

  4. Optimization of ZnO-NPs to Investigate Their Safe Application by Assessing Their Effect on Soil Nematode Caenorhabditis elegans

    Science.gov (United States)

    Gupta, Shruti; Kushwah, Tanuja; Vishwakarma, Ashutosh; Yadav, Shweta

    2015-07-01

    Zinc oxide nanoparticles (ZnO-NPs) are increasingly receiving attention due to their widespread application in cosmetics, pigments and coatings. This has raised concerns in the public and scientific communities regarding their unexpected health effects. Toxicity effect of ZnO-NPs on the environment was assessed in the present study using Caenorhabditis elegans. Multiple toxicity end points including their mortality, behaviour, reproduction, in vitro distribution and expression of stress response mtl-1 and sod-1 genes were observed to evaluate safe application of ZnO-NPs. C. elegans were exposed to 10, 50, and 100 nm ZnO-NPs (0.1 to 2.0 g/l). Application of 10 nm ≥0.7g/l adversely affects the survivability of worms and was significantly not affected with exposure of 50 and 100 nm ≤1.0 g/l. However, reproduction was affected at much low concentration as compared to their survivability. LC50 was recorded 1.0 ± 0.06 (g/l) for 100 nm, 0.90 ± 0.60 for 50 nm and 0.620 ± 0.08 for 10 nm. Expression of mtl-1 and sod-1 was significantly increased with application of 10 nm ≥0.7g/l and significantly unaffected with exposure of 50 and 100 nm at the same concentration. ZnO-NPs (10 nm) had shown even distribution extended nearly the entire length of the body. The distribution pattern of ZnO-NPs indicates that the intestine is the major target tissues for NP toxicity. Study demonstrates that small-sized (10 nm) ZnO-NPs ≥0.7g/l is more toxic than larger-sized particles. This may be suggested on the basis of available data; application of 50 and 100 nm ≤1.0 g/l ZnO-NPs may be used to the environment as this shows no significant toxicity. However, further calibration is warranted to explore safe dose on soil compartments prior to their field application.

  5. Unraveling the complex behavior of AgNPs driving NP-Cell interactions and toxicity to algal cells

    DEFF Research Database (Denmark)

    Malysheva, Anzhela; Voelcker, Nicolas; Holm, Peter Engelund

    2016-01-01

    toxicity test. In particular, we investigated NP aggregation and dissolution by time-resolved inductively coupled plasma mass spectrometry and ultrafiltration and performed mass balance measurements to study the distribution of Ag in the test system. We also determined the amount of extra......- and intracellular Ag by chemically etching AgNPs on the surface of algal cells and used dark field microscopy for their imaging. We observed that positively charged branched polyethilenimine (bPEI)-coated AgNPs tend to aggregate in the presence of algae and interact with test vessels and algal cells, while citrate...

  6. Antioxidant enzyme activities as biomarkers of fluvial biofilm to ZnO NPs ecotoxicity and the Integrated Biomarker Responses (IBR) assessment.

    Science.gov (United States)

    Hou, Jun; You, Guoxiang; Xu, Yi; Wang, Chao; Wang, Peifang; Miao, Lingzhan; Dai, Shanshan; Lv, Bowen; Yang, Yangyang

    2016-11-01

    The presence of ZnO nanoparticles (ZnO NPs) in natural waters has raised concerns about their environmental impacts, but the potential influences of ZnO NPs on fluvial biofilm have not been reported. In this study, the utility of antioxidant enzyme activities (AEA) as biomarkers of fluvial biofilm to ZnO NPs toxicity and a method that combines AEA into an index of "Integrated Biomarker Responses (IBR)" were studied. Compared with the absence of ZnO NPs, scanning electron microscopy (SEM) images revealed that a large amount of ZnO NPs were adsorbed onto biofilm and these NPs exerted adverse effects on the viability of bacteria in biofilm. The production of reactive oxygen species (ROS) with high concentrations (30 and 100mg/L) of ZnO NPs exposure reached to 184% and 244% of the control, while no cell leakage and membrane damage were observed. After exposure to ZnO NPs for 0.25 and 3 days, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR), glutathione peroxidase (GSH-Px) were significantly increased, respectively. At the end of exposure period (21 days), the AEA with the presence of 1mg/L ZnO NPs exposure were comparable to the control, while most of those in high concentrations of ZnO NPs were decreased. The results of IBR showed that the biofilm can adapt to 1mg/L ZnO NPs exposure, while be seriously damaged by 30 and 100mg/L ZnO NPs after 3 and 0.25 days. IBR can be used as an appropriate evaluation system of the toxicity effects of ZnO NPs on fluvial biofim.

  7. Antimicrobial kinetics of Alstonia scholaris bark extract-mediated AgNPs

    Science.gov (United States)

    Supraja, N.; Prasad, T. N. V. K. V.; David, E.; Giridhara Krishna, T.

    2016-06-01

    Nanobiotechnology is considered as one of the important branches of nanotechnology, and research on synthesis of nanoscale materials, silver in particular, using plant and plant parts has been progressing rapidly. Herein, we used bark extract of Alstonia scholaris one of the most important medicinal plants to synthesize silver nanoparticles (AgNPs) which exhibited excellent antimicrobial properties against biofilm formed in drinking water PVC pipes. The biosynthesis of silver nanoparticles was done by treating 90 mL of 1 mM AgNO3 aqueous solution with 10 mL of 5 % bark extract. As-prepared silver nanoparticles were characterized using the biophysical techniques such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, transmission electron microscopy, and dynamic light scattering for the measurement of hydrodynamic diameter and zeta potential. The kinetics of the antimicrobial activity against PVC biofilm of prepared silver nanoparticles were done using comparative solution suspension time-killing assessments and which are evidenced in Epi-fluorescent microscopic observations.

  8. Lignin-AuNPs liquid marble for remotely-controllable detection of Pb2+

    Science.gov (United States)

    Han, Guocheng; Wang, Xiaoying; Hamel, Jonathan; Zhu, Hongli; Sun, Runcang

    2016-12-01

    This work reported the green and facile fabrication of a versatile lignin-AuNP composite, which was readily and remotely encapsulated to form novel liquid marbles. The marbles can stay suspended in water, and show excellent photothermal conversion properties, as well as visual detection and adsorption towards Pb2+. More importantly, the marbles can simultaneously remotely detect and adsorb Pb2+ via co-precipitation by simply controlling the near infrared (NIR) irradiation. It is believed that the remotely-controllable NIR-responsive lignin-AuNPs liquid marble can be used in Pb2+-related reactions. The liquid marble can be placed in the system at the very beginning of the reaction and stably stays on the surface until the reaction has ended. After reacting, upon remote NIR irradiation, the liquid marble bursts to adsorb Pb2+, and the residual Pb2+ can be collected. This facile manipulation strategy does not use complicated nanostructures or sophisticated equipment, so it has potential applications for channel-free microfluidics, smart microreactors, microengines, and so on.

  9. 78 FR 14589 - Notice of Open Public Meetings for the National Park Service (NPS) Alaska Region's Subsistence...

    Science.gov (United States)

    2013-03-06

    ... Stat. 770), the NPS is hereby giving notice that the Gates of the Arctic National Park Subsistence... the Arctic National Park SRC Meeting Date and Location: The Gates of the Arctic National Park SRC will... School in Ambler, AK. SRC meeting locations and dates may change based on inclement weather...

  10. 77 FR 14828 - Notice of Public Meeting for the National Park Service (NPS) Alaska Region's Subsistence Resource...

    Science.gov (United States)

    2012-03-13

    ... changed based on inclement weather or exceptional circumstances. Gates of the Arctic National Park SRC Meeting Date and Location: The Gates of the Arctic National Park SRC will meet in Anaktuvuk Pass, Alaska... the Arctic National Park SRC will meet to develop and continue work on NPS subsistence...

  11. Cocos nucifera coir-mediated green synthesis of Pd NPs and its investigation against larvae and agricultural pest.

    Science.gov (United States)

    Elango, Ganesh; Mohana Roopan, Selvaraj; Abdullah Al-Dhabi, Naif; Arasu, Mariadhas Valan; Irukatla Damodharan, Kasinathan; Elumalai, Kuppuswamy

    2016-12-01

    In recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. Cocos nucifera (C. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. Cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help C. nucifera coir, Palladium nanoparticles (Pd NPs) were synthesized. Green-synthesized spherical-shape Pd NPs were over layered by secondary metabolites from C. nucifera coir extract and with an average particle size of 62 ± 2 nm, which were confirmed by morphological analysis. Eco-friendly mediated Pd NPs were further subjected to several biological applications like larvicidal against Aedes aegypti (A. aegypti) and anti-feedent, ovicidal, and oviposition deterrent against agricultural pest Callasobruchus maculates (C. maculates) and compared with C. nuciferacoir methanolic extract, which results in LC50 value of 288.88 ppm and LC90 value of 483.06 ppm using LSD-Tukey's test against dengue vector (A. aegypti). Cocos nucifera coir methanolic extract shows significant output while compared with Pd NPs towards anti-feedent assays; ovicidal activity and oviposition deterrent were discussed here.

  12. 75 FR 65377 - Notice of Public Meeting for the National Park Service (NPS) Alaska Region's Subsistence Resource...

    Science.gov (United States)

    2010-10-22

    ... subsistence hunting program recommendations and other related subsistence management issues. The NPS SRC... Station Hotel, 1717 University Avenue, Fairbanks, AK 99709, (907) 479-3650. The meeting may end early if... Manager's Report 10. Park Staff Reports a. Resource Management Update b. Ranger Division Update c...

  13. Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs on Cells

    Directory of Open Access Journals (Sweden)

    Smitha Rao

    2014-01-01

    Full Text Available Nanoparticles (NPs have been introduced as a suitable alternative in many in vivo bioapplications. The risks of utilizing nanoparticles continue to be an ongoing research. Furthermore, the various chemicals used in their synthesis influence the cytotoxic effects of nanoparticles. We have investigated the cytotoxicity of Porous Hollow Au Nanoparticles (PHAuNPs on cancer cell lines PC-3, PC-3ML, and MDA-MB-231 and the normal cell line PNT1A. Cell proliferation for the different cells in the presence of different concentrations of the PHAuNPs was assessed after 24 hours and 72 hours of incubation using MTT assay. The study also included the cytotoxic evaluation of pegylated PHAuNPs. Identical cell seeding densities, particle concentrations, and incubation times were employed for these two types of Au nanoparticles. Our results indicated that (1 impact on cell proliferation was concentration dependent and was different for the different cell types without cellular necrosis and (b cellular proliferation might be impacted more based on the cell line.

  14. Zinc (hydr)oxide/graphite oxide/AuNPs composites: role of surface features in H₂S reactive adsorption.

    Science.gov (United States)

    Giannakoudakis, Dimitrios A; Bandosz, Teresa J

    2014-12-15

    Zinc hydroxide/graphite oxide/AuNPs composites with various levels of complexity were synthesized using an in situ precipitation method. Then they were used as H2S adsorbents in visible light. The materials' surfaces were characterized before and after H2S adsorption by various physical and chemical methods (XRD, FTIR, thermal analysis, potentiometric titration, adsorption of nitrogen and SEM/EDX). Significant differences in surface features and synergistic effects were found depending on the materials' composition. Addition of graphite oxide and the deposition of gold nanoparticles resulted in a marked increase in the adsorption capacity in comparison with that on the zinc hydroxide and zinc hydroxide/AuNP. Addition of AuNPs to zinc hydroxide led to a crystalline ZnO/AuNP composite while the zinc hydroxide/graphite oxide/AuNP composite was amorphous. The ZnOH/GO/AuNPs composite exhibited the greatest H2S adsorption capacity due to the increased number of OH terminal groups and the conductive properties of GO that facilitated the electron transfer and consequently the formation of superoxide ions promoting oxidation of hydrogen sulfide. AuNPs present in the composite increased the conductivity, helped with electron transfer to oxygen, and prevented the fast recombination of the electrons and holes. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. 浅析NPS顾客忠诚度测评法%Analysis of NPS Customer Loyalty Evaluation Method

    Institute of Scientific and Technical Information of China (English)

    梁淑琴

    2015-01-01

    With the rapid development of mobile Internet, great changes have taken place in consumer behavior, coupled with the homogenization of products and the market is relatively saturated, how to improve customer loyalty in front of the operator. This paper introduces the NPS system, understand the advantages of NPS and NPS, combining with the practical work, the operator NPS promotion strategy, to improve customer loyalty, and promote the healthy development of enterprises.%面对移动互联网的飞速发展,用户消费行为发生了巨大的变化,加之产品同质化与市场相对饱和,如何提升客户忠诚度摆在了运营商的面前。文章通过介绍NPS体系,了解NPS的优点,并将NPS与实际工作相结合,提出运营商NPS提升策略,以提高客户忠诚度,促进企业良性发展。

  16. Application of the PJ and NPS evaporation duct models over the South China Sea (SCS) in winter.

    Science.gov (United States)

    Yang, Shaobo; Li, Xingfei; Wu, Chao; He, Xin; Zhong, Ying

    2017-01-01

    The detection of duct height has a significant effect on marine radar or wireless apparatus applications. The paper presents two models to verify the adaptation of evaporation duct models in the SCS in winter. A meteorological gradient instrument used to measure evaporation ducts was fabricated using hydrological and meteorological sensors at different heights. An experiment on the adaptive characteristics of evaporation duct models was carried out over the SCS. The heights of the evaporation ducts were measured by means of log-linear fit, Paulus-Jeske (PJ) and Naval Postgraduate School (NPS) models. The results showed that NPS model offered significant advantages in stability compared with the PJ model. According the collected data computed by the NPS model, the mean deviation (MD) was -1.7 m, and the Standard Deviation (STD) of the MD was 0.8 m compared with the true value. The NPS model may be more suitable for estimating the evaporation duct height in the SCS in winter due to its simpler system characteristics compared with meteorological gradient instruments.

  17. Solid-Phase Synthesis of New Trp(Nps-Containing Dipeptide Derivatives as TRPV1 Channel Blockers

    Directory of Open Access Journals (Sweden)

    Rosario González-Muñiz

    2010-07-01

    Full Text Available Trp(Nps-Lys-NH2 derivatives, bearing alkyl or guanidine groups either at the N–terminus or on the Lys side-chain or at both positions were conveniently prepared on solid-phase and evaluated as TRPV1 channel antagonists.

  18. Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay.

    Science.gov (United States)

    Maisano, Maria; Cappello, Tiziana; Catanese, Eva; Vitale, Valeria; Natalotto, Antonino; Giannetto, Alessia; Barreca, Davide; Brunelli, Elvira; Mauceri, Angela; Fasulo, Salvatore

    2015-10-01

    The embryotoxicity of CuO NPs was evaluated in the black sea urchin Arbacia lixula embryos, by using 24-well plates. Fertilized eggs were exposed to five doses of CuO NPs ranging from 0.07 to 20 ppb, until pluteus stage. CuO NPs suspensions in artificial seawater formed agglomerates of 80-200 nm size, and copper uptake was 2.5-fold up in larvae exposed to high NP concentrations in respect to control. Developmental delay and morphological alteration, including skeletal abnormalities, were observed, as well as impairment in cholinergic and serotonergic nervous systems. These findings suggest the potential of CuO NPs to interfere with the normal neurotransmission pathways, thus affecting larval morphogenesis. Overall, the embryotoxicity tests are effective for evaluation of nanoparticle effects on the health of aquatic biota. Furthermore, as the black sea urchin A. lixula demonstrated to be vulnerable to NP exposure, it may be a valid bioindicator in marine biomonitoring and ecotoxicological programmes.

  19. Preparation of reduced graphene oxide/meso-TiO2/AuNPs ternary composites and their visible-light-induced photocatalytic degradation n of methylene blue

    Science.gov (United States)

    Yang, Yongfang; Ma, Zheng; Xu, Lidong; Wang, Hefang; Fu, Nian

    2016-04-01

    Reduced graphene oxide/meso-TiO2/AuNPs (RGO/meso-TiO2/AuNPs) ternary composites were prepared via the addition of graphene oxide to the dispersion of meso-TiO2/AuNPs under hydrothermal conditions. The structure and the morphology of the RGO/meso-TiO2/AuNPs materials were characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The photocatalytic activity of RGO/meso-TiO2/AuNPs was evaluated by degradation of methyl blue (MB) under visible-light illumination. The ternary composites present an extended light absorption range, efficient charge separation properties, high adsorption ability for MB and high photocatalytic degradation activity of MB compared to the meso-TiO2 and meso-TiO2/AuNPs.

  20. Evaluation of AgClNPs@SBA-15/IL nanoparticle-induced oxidative stress and DNA mutation in Escherichia coli.

    Science.gov (United States)

    Karimi, Farrokh; Dabbagh, Somayyeh; Alizadeh, Sina; Rostamnia, Sadegh

    2016-08-01

    The bactericidal effects of silver nanoparticles have been demonstrated in the past years. Recently, the new antimicrobial compounds of silver nanoparticles with different formulations have been developed. In this work, AgClNPs@SBA-15/IL as a new compound of Ag nanoparticles, was synthesized and characterized by XRD, TEM, SEM, FTIR, and EDX. The antibacterial activity and the molecular mechanism effects of AgClNPs@SBA-15/IL nanoparticles (SNPs) on Escherichia coli DH5α cells were investigated by analyzing the growth inhibitory, H2O2 level, catalase activity, DNA mutation, and plasmid copy number following treatment with AgClNPs@SBA-15/IL nanoparticles. In experimental results, the minimum inhibitory concentration (MIC) was observed in 75 μg/ml and the antibacterial efficacy (ABE) in CFU analysis was estimated 95.3 %. In bacterial cells treated with 75 and 100 μg/ml, H2O2 level significantly increased and catalase activity decreased compared with control. The random amplified polymorphic DNA (RAPD) was used to evaluate the effect of AgClNPs@SBA-15/IL nanoparticles in DNA damages and mutation in E. coli genome. RADP-PCR results indicated different banding patterns including appearance or disappearance of bands and differences in their intensity. Cluster analysis of the RAPD-PCR results based on genetic similarity showed genetic difference between E. coli cells treated with AgClNPs@SBA-15/IL nanoparticles, and control and phylogenetic tree were divided to two clusters. Plasmid copy number analysis indicated that after 8 h incubation of E. coli cells with 50, 75, and 100 μg/ml AgClNPs@SBA-15/IL nanoparticles, copy number of pET21a (+) significantly decreased compared with control which indicating DNA replication inhibition by Ag nanoparticles. In conclusion, the results of this study indicated that AgClNPs@SBA-15/IL nanoparticles can be used as an effective bactericidal agent against bacterial cells.

  1. Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

    Science.gov (United States)

    Azeez, Luqmon; Lateef, Agbaje; Adebisi, Segun A.

    2017-02-01

    This study investigates the influence of different concentrations of AgNPs biologically synthesized using pod extract of Cola nitida on antioxidant activity, phenolic contents, flavonoid contents and compositions of Amaranthus caudatus L. AgNPs of 25, 50, 75, 100 and 150 ppm were utilized in growing A. caudatus while water was used as control. Delayed germination for two days was observed for A. caudatus grown with 150 ppm of AgNPs, while others showed no difference. There were 43.3, 38.7, 26.7 and 6.48% improvements in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity of A. caudatus grown with 25, 50, 75 and 100 ppm of AgNPs, respectively, compared to control. Antioxidant activity of A. caudatus grown with AgNPs reduced with increase in the concentrations of AgNPs. A. caudatus grown with 50 ppm of AgNPs was the most potent with the least IC50 of 0.67 mg/ml. Significant improvements obtained for phenolic and flavonoid contents grown with AgNPs were concentration dependent. Enhancements of 21.9, 68.19, and 1.98% in phenolic contents were achieved in treatments with 25, 50 and 75 ppm AgNPs, respectively, while 32.58, 35.80, and 7.20% improvement in flavonoids were obtained for 25, 50 and 100 ppm treatments, respectively. Kaempferol and quercetin were the most abundant flavonoids in A. caudatus treated with 50 ppm of AgNPs, showing the highest flavonoid composition. This further confirms A. caudatus grown with 50 ppm of AgNPs as the most potent. This study has shown that concentration-dependent AgNPs can be used to boost antioxidant activity and phytochemical contents of vegetables.

  2. Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

    Science.gov (United States)

    Azeez, Luqmon; Lateef, Agbaje; Adebisi, Segun A.

    2017-01-01

    This study investigates the influence of different concentrations of AgNPs biologically synthesized using pod extract of Cola nitida on antioxidant activity, phenolic contents, flavonoid contents and compositions of Amaranthus caudatus L. AgNPs of 25, 50, 75, 100 and 150 ppm were utilized in growing A. caudatus while water was used as control. Delayed germination for two days was observed for A. caudatus grown with 150 ppm of AgNPs, while others showed no difference. There were 43.3, 38.7, 26.7 and 6.48% improvements in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity of A. caudatus grown with 25, 50, 75 and 100 ppm of AgNPs, respectively, compared to control. Antioxidant activity of A. caudatus grown with AgNPs reduced with increase in the concentrations of AgNPs. A. caudatus grown with 50 ppm of AgNPs was the most potent with the least IC50 of 0.67 mg/ml. Significant improvements obtained for phenolic and flavonoid contents grown with AgNPs were concentration dependent. Enhancements of 21.9, 68.19, and 1.98% in phenolic contents were achieved in treatments with 25, 50 and 75 ppm AgNPs, respectively, while 32.58, 35.80, and 7.20% improvement in flavonoids were obtained for 25, 50 and 100 ppm treatments, respectively. Kaempferol and quercetin were the most abundant flavonoids in A. caudatus treated with 50 ppm of AgNPs, showing the highest flavonoid composition. This further confirms A. caudatus grown with 50 ppm of AgNPs as the most potent. This study has shown that concentration-dependent AgNPs can be used to boost antioxidant activity and phytochemical contents of vegetables.

  3. The Neutrophil-Platelet Score (NPS Predicts Survival in Primary Operable Colorectal Cancer and a Variety of Common Cancers.

    Directory of Open Access Journals (Sweden)

    David G Watt

    Full Text Available Recent in-vitro studies have suggested that a critical checkpoint early in the inflammatory process involves the interaction between neutrophils and platelets. This confirms the importance of the innate immune system in the elaboration of the systemic inflammatory response. The aim of the present study was to examine whether a combination of the neutrophil and platelet counts were predictive of survival in patients with cancer.Patients with histologically proven colorectal cancer who underwent potentially curative resection at a single centre between March 1999 and May 2013 (n = 796 and patients with cancer from the Glasgow Inflammation Outcome Study, who had a blood sample taken between January 2000 and December 2007 (n = 9649 were included in the analysis.In the colorectal cancer cohort, there were 173 cancer and 135 non-cancer deaths. In patients undergoing elective surgery, cancer-specific survival (CSS at 5 years ranged from 97% in patients with TNM I disease and NPS = 0 to 57% in patients with TNM III disease and NPS = 2 (p = 0.019 and in patients undergoing elective surgery for node-negative colon cancer from 98% (TNM I, NPS = 0 to 65% (TNM II, NPS = 2 (p = 0.004. In those with a variety of common cancers there were 5218 cancer and 929 non-cancer deaths. On multivariate analysis, adjusting for age and sex and stratified by tumour site, incremental increase in the NPS was significantly associated with poorer CSS (p<0.001.The neutrophil-platelet score predicted survival in a variety of common cancers and highlights the importance of the innate immune system in patients with cancer.

  4. Electrochemical preparation of Au-PtNPs/SWNT modified electrode and its application%Au-PtNPs/SWNT复合材料修饰电极的电化学制备及其应用

    Institute of Scientific and Technical Information of China (English)

    李春兰; 朱效华; 朱旭; 徐茂田

    2013-01-01

    The Au-PtNPs/SWNT modified electrode was prepared by an electrochemical method at room temperature. The surface morphology of the modified electrode was measured by AFM and the results indicate that there are dispersions and high loadings of Au-Pt nanoparticles on SWNT. Under the optimal modification conditions(Electrodeposition of SWNT for 30 s,soaking in H2PtO6 for 10 minutes,multi-step deposition of gold nanoparticles for 45 cycles(glucose) or 30 cycles( methanol) ) ,glucose and methanol can be electrocatalytically oxidized on the surface of Au-PtNPs/SWNT modified electrode in alkaline environment Thus,the Au-Pt-NPs/SWNT/GCE is expected to be applied as a nonenzymatic glucose sensor or in the filed of methanol fuel cells.%在室温条件下,利用恒电位吸附法和多电位阶跃法制备了金-铂纳米粒子(Au-PtNPs)/单壁碳纳米管(SWNT)复合材料修饰电极,并利用电化学方法和原子力显微镜(AFM)对其进行了表征.结果表明:Au-Pt-NPs可很好的结合在SWNT表面,在该电极的最佳修饰条件下(SWNT分散液中电沉积30 s,H2PtO6中浸泡10min,循环阶跃沉积金纳米粒子45次(葡萄糖)或30次(甲醇))可以较好的电催化氧化碱性环境中的葡萄糖及甲醇,有望在葡萄糖无酶传感器及甲醇燃料电池中得到应用.

  5. Piezoresistive Sensor with High Elasticity Based on 3D Hybrid Network of Sponge@CNTs@Ag NPs.

    Science.gov (United States)

    Zhang, Hui; Liu, Nishuang; Shi, Yuling; Liu, Weijie; Yue, Yang; Wang, Siliang; Ma, Yanan; Wen, Li; Li, Luying; Long, Fei; Zou, Zhengguang; Gao, Yihua

    2016-08-31

    Pressure sensors with high elasticity are in great demand for the realization of intelligent sensing, but there is a need to develope a simple, inexpensive, and scalable method for the manufacture of the sensors. Here, we reported an efficient, simple, facile, and repeatable "dipping and coating" process to manufacture a piezoresistive sensor with high elasticity, based on homogeneous 3D hybrid network of carbon nanotubes@silver nanoparticles (CNTs@Ag NPs) anchored on a skeleton sponge. Highly elastic, sensitive, and wearable sensors are obtained using the porous structure of sponge and the synergy effect of CNTs/Ag NPs. Our sensor was also tested for over 2000 compression-release cycles, exhibiting excellent elasticity and cycling stability. Sensors with high performance and a simple fabrication process are promising devices for commercial production in various electronic devices, for example, sport performance monitoring and man-machine interfaces.

  6. Nata de coco (NDC) hydrogel as nanoreactors for preparation iron nanoparticles (FeNps) from ferrocenium reduction

    Energy Technology Data Exchange (ETDEWEB)

    Andarini, Mellissa; Lazim, Azwan [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor (Malaysia)

    2014-09-03

    This study focuses on hydrogel as nano template to produce iron nanoparticles (FeNps). Radical polymerization was used to synthesize the hydrogel from nata de coco (NDC-g-PAA). Ferrocenium (FcCL) with 1 × 10{sup −4} g/ml has successfully incorporated with NDC-g-PAA hydrogel system and reduce using sodium hydroxide (NaOH) at different concentrations. Transmission electron microscopy (TEM) result demonstrates that the size of FeNps produced was about 5 – 20 nm. Morphological analysis of hydrogel is carried out by scanning electron microscopy (SEM), SEM-EDEX is used to determine percentage of iron (Fe) in hydrogel. The results offer a wide range of application in various areas, especially the use of hydrogel system as a responsive template.

  7. Long-term effects of cupric oxide nanoparticles (CuO NPs) on the performance, microbial community and enzymatic activity of activated sludge in a sequencing batch reactor.

    Science.gov (United States)

    Wang, Sen; Li, Zhiwei; Gao, Mengchun; She, Zonglian; Ma, Bingrui; Guo, Liang; Zheng, Dong; Zhao, Yangguo; Jin, Chunji; Wang, Xuejiao; Gao, Feng

    2017-02-01

    The long-term effects of cupric oxide nanoparticles (CuO NPs) on the performance, microbial activity and microbial community of activated sludge were investigated in a sequencing batch reactor (SBR). The SBR performance had no evident change at 0-10 mg/L CuO NPs, whereas the CuO NPs concentration at 30-60 mg/L affected the COD, NH4(+)-N and soluble orthophosphate (SOP) removal, nitrogen and phosphorus removal rate and microbial enzymatic activity of activated sludge. Some CuO NPs might be absorbed on the surface of activated sludge or penetrate the microbial cytomembrane into the microbial cell interior of activated sludge. Compared to 0 mg/L CuO NPs, the reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) release increased by 43.6% and 56.4% at 60 mg/L CuO NPs, respectively. The variations of ROS production and LDH release demonstrated that CuO NPs could induce the toxicity towards the microorganisms and destroy the integrity of microbial cytomembrane in the activated sludge. High throughput sequencing of 16S rDNA indicated that CuO NPs could evidently impact on the microbial richness, diversity and composition of activated sludge in the SBR.

  8. Aminated polyethersulfone-silver nanoparticles (AgNPs-APES) composite membranes with controlled silver ion release for antibacterial and water treatment applications.

    Science.gov (United States)

    Haider, M Salman; Shao, Godlisten N; Imran, S M; Park, Sung Soo; Abbas, Nadir; Tahir, M Suleman; Hussain, Manwar; Bae, Wookeun; Kim, Hee Taik

    2016-05-01

    The present study reports the antibacterial disinfection properties of a series of silver nanoparticle (AgNP) immobilized membranes. Initially, polyethersulfone (PES) was functionalized through the introduction of amino groups to form aminated polyethersulfone (NH2-PES, APES). AgNPs were then coordinately immobilized on the surface of the APES composite membrane to form AgNPs-APES. The properties of the obtained membrane were examined by FT-IR, XPS, XRD, TGA, ICP-OES and SEM-EDAX analyses. These structural characterizations revealed that AgNPs ranging from 5 to 40 nm were immobilized on the surface of the polymer membrane. Antibacterial tests of the samples showed that the AgNPs-APES exhibited higher activity than the AgNPs-PES un-functionalized membrane. Generally, the AgNPs-APES 1 cm × 3 cm strip revealed a four times longer life than the un-functionalized AgNPs polymer membranes. The evaluation of the Ag(+) leaching properties of the obtained samples indicated that approximately 30% of the AgNPs could be retained, even after 12 days of operation. Further analysis indicated that silver ion release can be sustained for approximately 25 days. The present study provides a systematic and novel approach to synthesize water treatment membranes with controlled and improved silver (Ag(+)) release to enhance the lifetime of the membranes.

  9. Nonpoint Source Pollution Control Projects Grants (Section 319) - 2008 active projects

    Data.gov (United States)

    U.S. Environmental Protection Agency — The California Nonpoint Source (NPS) Program allocates about $4.5 million of CWA Section 319 funding from the U.S. Environmental Protection Agency annually to...

  10. NPS comparison of anatomical noise characteristics in mammography, tomosynthesis, and breast CT images using power law metrics

    Science.gov (United States)

    Chen, Lin; Boone, John M.; Nosratieh, Anita; Abbey, Craig K.

    2011-03-01

    Digital mammography is the current standard for breast cancer screening, however breast tomosynthesis and breast CT (bCT) have been studied in clinical trials. At our institution, 30 women (BIRADS 4 and 5) underwent IRB-approved imaging by mammography, breast tomosynthesis, and bCT on the same day. Twenty three data sets were used for analysis. The 2D noise power spectrum (NPS) was computed and averaged for each data set. The NPS was computed for different slice thicknesses of dx × N, where dx ~ 0.3 mm and N=1-64, on the bCT data. Each 2D NPS was radially averaged, and the 1D data were fit using a power law function as proposed by Burgess: NPS(f) = αf-β. The value of β was determined over a range of frequencies corresponding to anatomical noise, for each patient and each modality. Averaged over the 30 women (26 for bCT, 28 for tomosynthesis, 28 for mammography), for mammography β=3.06 (0.25), for CC tomosynthesis β=2.91 (0.35), and for axial bCT β=1.72 (0.47). For sagittal bCT β=1.77 (0.36) and for coronal bCT, β=1.88 (0.45). The computation of β versus slice thickness on the coronal bCT data set led to β~1.7 for N=1, asymptotically reaching β ~ 3 for larger slice thickness. These results suggest that there is a fundamental difference in breast anatomic noise as characterized by β, between thin slices (<2 mm) and thicker slices. Tomosynthesis was found to have anatomic noise properties closer to mammography than breast CT, most likely due to the relatively thick slice sensitivity profile of tomosynthesis.

  11. Design, simulation, and experimental verification of a computer model and enhanced position estimator for the NPS AUV II

    OpenAIRE

    Warner, David C.

    1991-01-01

    A full six-degree-of-freedom computer model of the Naval Postgraduate School Autonomous Underwater Vehicle (NPS AUV II) is developed. Hydrodynamic Coefficients are determined by geometric similarity with an existing swimmer delivery vehicle and analysis of initial open loop AUV II trials. Comparisons between simulated and experimental results demonstrate the validity of the model and the techniques used. A reduced order observer of lateral velocity was produced to provide an input for an enha...

  12. Abnormal response to stress and impaired NPS-induced hyperlocomotion, anxiolytic effect and corticosterone increase in mice lacking NPSR1

    OpenAIRE

    Zhu, Hongyan; Mingler, Melissa K; McBride, Melissa L.; Murphy, Andrew J.; Valenzuela, David M.; Yancopoulos, George D.; Williams, Michael T.; Vorhees, Charles V.; Rothenberg, Marc E.

    2010-01-01

    NPSR1 is a G protein coupled receptor expressed in multiple brain regions involved in modulation of stress. Central administration of NPS, the putative endogenous ligand of NPSR1, can induce hyperlocomotion, anxiolytic effects and activation of the HPA axis. The role of NPSR1 in the brain remains unsettled. Here we used NPSR1 gene-targeted mice to define the functional role of NPSR1 under basal conditions on locomotion, anxiety- and/or depression-like behavior, corticosterone levels, acoustic...

  13. STUDYING THE INTERSTELLAR MEDIUM AND THE INNER REGION OF NPS/LOOP 1 WITH SHADOW OBSERVATIONS TOWARD MBM36

    Energy Technology Data Exchange (ETDEWEB)

    Ursino, E.; Galeazzi, M.; Liu, W., E-mail: galeazzi@physics.miami.edu [Physics Department, University of Miami, Coral Gables, FL 33155 (United States)

    2016-01-01

    We analyzed data from a shadow observation of the high density molecular cloud MBM36 (l ∼ 4°, b ∼ 35°) with Suzaku. MBM36 is located in a region that emits relatively weakly in the 3/4 keV band compared to the surrounding North Polar Spur (NPS)/Loop 1 structure and the Galactic Bulge (GB). The contrast between high and low density targets in the MBM36 area allows one to separate the local and distant contributors to the soft diffuse X-ray background, providing a much better characterization of the individual components compared to single pointing observations. We identify two non-local thermal components, one at kT ≈ 0.12 keV and one at kT ≈ 0.29 keV. The colder component matches well with models of emission from the higher latitude region of the GB. The emission of the warmer component is in agreement with models predicting that the NPS is due to a hypershell from the center of the Milky Way. Geometrical and pressure calculations rule out a nearby bubble as responsible for the emission associated with the NPS. Any Galactic Halo/circumgalactic halo emission, if present, is outshined by the other components. We also report an excess emission around 0.9 keV, likely due to an overabundance of Ne ix.

  14. Development of a lidar for integration with the Naval Postgraduate School Infrared Search and Target Designation (NPS-IRSTD) system

    Science.gov (United States)

    Regush, Murray M.

    1993-06-01

    A lidar was designed and manufactured at the Naval Postgraduate School, Monterey, CA, to provide range information to atmospheric features, such as clouds. It is further planned to integrate the lidar with the NPS-IRSTD system at some future date. The NPS-IRSTD uses two vertical linear focal plane arrays for target detection and target direction can be determined very accurately but the system does not provide any useful range information. The lidar was proposed as the solution for this shortcoming. The lidar used a frequency-doubled Nd:YAG laser which had an energy output of 2 millijoules. The laser beam was expanded to 17.75 inches using a Dall-Kirkham telescope to operate within laser safety limitations. The theoretical analysis of the 'Klett' method for the inversion of lidar returns was derived and a MATLAB program was written to demonstrate the process. A daytime and nighttime maximum range equation for the lidar was developed. The considerations for integrating the lidar with the NPS-IRSTD were listed and a solution was proposed to obtain the mean extinction coefficient along the path in the infrared spectrum using the lidar inversion extinction coefficient profile at 532 nanometers.

  15. BIOSYNTHESIS OF AgNPs WITH THREE WIDESPREAD LOAM FUNGI VIA ASPERGILLUS FUMIGATUS, FUSARIUM SPP. RHIZOPUS SPP.

    Directory of Open Access Journals (Sweden)

    P. SHIVAKUMAR SINGH

    2016-05-01

    Full Text Available Objectives: Biosynthesis of silver nanoparticles using fungi Aspergillus fumigatus, Fusarium spp. and Rhizopus spp. for the potential synthesis of metal nanoparticles. Methods: The standard techniques have been followed for the AgNPs synthesis characterizations. Spectral analysis of UV-visible spectroscopy, Transmission Electron Microscopy, X-ray diffraction studies, energy dispersive X-ray, Fourier transforms infrared spectroscopy. Results: The hurried decline of silver (Ag+ ions was monitored using a UV-visible spectrophotometer and showed the formation of silver nanoparticles within 28 minutes. Transmission electron microscopy (TEM showed that the synthesized silver nanoparticles are varied from 15-50 nm and have the varying in shapes like round, rod, uneven. Further, the XRD analysis confirms the nano-crystalline phase of the silver structure. FTIR examinations confirm the Silver particles. The present study, it reveals the increasing broth concentration increases the rate of reduction and decreases the particle size. Conclusion: The AgNPs were biologically synthesized using isolated fungal species biomass from the soil of loam. The cell filtrate of fungi was challenged with 1mm Silver nitrate, change of mixture from colorless to orange-brown indicates the synthesis of AgNPs in the reaction mixture. The isolated fungi are an important producer of Silver nanoparticles.

  16. Studying the Interstellar Medium and the inner region of NPS/Loop 1 with shadow observations toward MBM36

    CERN Document Server

    Ursino, E; Liu, W

    2015-01-01

    We analyzed data from a shadow observation of the high density molecular cloud MBM36 (l~4{\\deg}, b~35{\\deg}) with Suzaku. MBM36 is located in a region that emits relatively weakly in the 3/4~keV band, compared to the surrounding NPS/Loop 1 structure and the Galactic Bulge. The contrast between a high and low density targets in the MBM36 area allows one to separate the local and distant contributors to the Soft Diffuse X-ray Background, providing a much better characterization of the individual components compared to single pointing observations. We identify two non-local thermal components, one at kT~0.12 keV and one at kT~0.29keV. The colder component matches well with models of emission from the higher latitude region of the Galactic Bulge. The emission of the warmer component is in agreement with models predicting that the NPS is due to a hypershell from the center of the Milky Way. Geometrical and pressure calculations rule out a nearby bubble as responsible for the emission associate with the NPS. Any Ga...

  17. Fluorescence turn-on recognition of chiral amino acids using dye incorporated β-CD functionalized AuNPs assembly

    Energy Technology Data Exchange (ETDEWEB)

    Aswathy, B., E-mail: aswathybv@gmail.com; Sony, G., E-mail: emailtosony@gmail.com

    2014-10-15

    An assembly of dye incorporated β-cyclodextrin (βCD) functionalized AuNPs for the fluorescent probing of chiral amino acids is presented. Gold nanoparticles (AuNPs) possessing a high extinction coefficient function can be used as excellent fluorescent quenchers in AuNP–fluorophore system. Inclusion of fluorescein (FL) into β-cyclodextrin (βCD) makes energy transfer to occur through the donor and quencher nearby. This energy transfer switches off by virtue of the analyte induced release of FL from β-CD cavity, which results in the fluorescence recovery of the quenched dye. Analysis suggests that the assembly of AuNPs–βCDs–FL is effective as a turn-on fluorescent probe for the chiroselective optical discrimination between D,L-tryptophan, D,L-phenyl alanine and D,L-tyrosine. The detection limits for analyzing L-tryptophan, L-phenyl alanine and L-tyrosine were found to be 0.59, 1.2 and 1.5 μM respectively. - Highlights: • Fluorescence quenching AuNP–βCD–dye assembly via energy transfer. • Energy transfer from dye to AuNPs is a SET process. • Fluorescence turn-on detection of amino acids by the competitive binding method. • Chiroselective discrimination between enantiomeric amino acids.

  18. An Electrochemical Microsensor Based on a AuNPs-Modified Microband Array Electrode for Phosphate Determination in Fresh Water Samples

    Directory of Open Access Journals (Sweden)

    Fangfang Wang

    2014-12-01

    Full Text Available This work describes the fabrication, characterization, and application of a gold microband array electrode (MAE for the determination of phosphate in fresh water samples. The working principle of this MAE is based on the reduction of a molybdophosphate complex using the linear sweep voltammetric (LSV method. The calibration of this microsensor was performed with standard phosphate solutions prepared with KH2PO4 and pH adjusted to 1.0. The microsensor consists of a platinum counter electrode, a gold MAE as working electrode, and an Ag/AgCl electrode as reference electrode. The microelectrode chips were fabricated by the Micro Electro-Mechanical System (MEMS technique. To improve the sensitivity, gold nanoparticles (AuNPs were electrodeposited on the working electrode. With a linear range from 0.02 to 0.50 mg P/L, the sensitivity of the unmodified microsensor is 2.40 µA per (mg P/L (R2 = 0.99 and that of the AuNPs-modified microsensor is 7.66 µA per (mg P/L (R2 = 0.99. The experimental results showed that AuNPs-modified microelectrode had better sensitivity and a larger current response than the unmodified microelectrode.

  19. The fate of iron nanoparticles in environmental waters treated with nanoscale zero-valent iron, FeONPs and Fe3O4NPs.

    Science.gov (United States)

    Peeters, Kelly; Lespes, Gaëtane; Zuliani, Tea; Ščančar, Janez; Milačič, Radmila

    2016-05-01

    Among the different nanoparticles (NPs) that are used in the remediation of contaminated environmental waters, iron nanoparticles (FeNPs) are the most frequently applied. However, if these FeNPs remain in the waters after the treatment, they can cause a hazard to the environment. In this work the time dependent size distribution of iron particles was investigated in Milli-Q water, forest spring water and landfill leachate after a variety of FeNP treatments. The efficiency of the metal removal by the FeNPs was also examined. The concentrations of the metals in the aqueous samples were determined before and after the nano-remediation by inductively coupled plasma mass spectrometry (ICP-MS). The data revealed that the settling and removal of the FeNPs after the treatment of the waters was related to the sample characteristics and the ways of dispersing the NPs. When mixing was used for the dispersion, the nano zero-valent iron (nZVI), FeONPs and Fe3O4NPs settled quickly in the Milli-Q water, the forest spring water and the landfill leachate. Dispersion with tertramethylammonium hydroxide (TMAH) resulted in a slower settling of the iron aggregates. In the Milli-Q and forest spring waters treated with FeONPs and dispersed by TMAH, the nanosized iron remained in solution as long as 24 h after the treatment and could represent a potential threat in environmental waters with a low ionic strength. The removal of the metals strongly depended on the type of FeNPs, the chemical speciation of the elements and the sample matrix. If the FeNPs are contaminated by a particular metal, this contaminant could be, during the NPs treatment, released into the water that is being remediated.

  20. Dynamic equilibrium of endogenous selenium nanoparticles in selenite-exposed cancer cells: a deep insight into the interaction between endogenous SeNPs and proteins.

    Science.gov (United States)

    Bao, Peng; Chen, Song-Can; Xiao, Ke-Qing

    2015-12-01

    Elemental selenium (Se) was recently found to exist as endogenous nanoparticles (i.e., SeNPs) in selenite-exposed cancer cells. By sequestrating critical intracellular proteins, SeNPs appear capable of giving rise to multiple cytotoxicity mechanisms including inhibition of glycolysis, glycolysis-dependent mitochondrial dysfunction, microtubule depolymerization and inhibition of autophagy. In this work, we reveal a dynamic equilibrium of endogenous SeNP assembly and disassembly in selenite-exposed H157 cells. Endogenous SeNPs are observed both in the cytoplasm and in organelles. There is an increase in endogenous SeNPs between 24 h and 36 h, and a decrease between 36 h and 72 h according to transmission electron microscopy results and UV-Vis measurements. These observations imply that elemental Se in SeNPs could be oxidized back into selenite by scavenging superoxide radicals and ultimately re-reduced into selenide; then the assembly and disassembly of SeNPs proceed simultaneously with the sequestration and release of SeNP high-affinity proteins. There is also a possibility that the reduction of elemental Se to selenide pathway may lie in selenite-exposed cancer cells, which results in the assembly and disassembly of endogenous SeNPs. Genome-wide expression analysis results show that endogenous SeNPs significantly altered the expression of 504 genes, compared to the control. The endogenous SeNPs induced mitochondrial impairment and decreasing of the annexin A2 level can lead to inhibition of cancer cell invasion and migration. This dynamic flux of endogenous SeNPs amplifies their cytotoxic potential in cancer cells, thus provide a starting point to design more efficient intracellular self-assembling systems for overcoming multidrug resistance.

  1. Multi-agency analysis of periphyton, fish, and benthic macroinvertebrate communities and the effects of point and non-point sources in the Indian Creek Watershed, Tazewell County, Virginia

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Chemical, physical, and biological indices were used in this study. Water and sediment samples were analyzed for EPA’s priority pollutants. Other measurements such...

  2. 生物移动床处理农村面源生活污水试验研究%TREATMENT OF RURAL NON-POINT SOURCE DOMESTIC WATER BY MOVING BED BIOFILM REACTOR

    Institute of Scientific and Technical Information of China (English)

    贾丽萍; 姜彬慧; 王一迪; 王海燕; 胡筱敏

    2016-01-01

    在实验室常温条件下,利用生物移动床(MBBR)处理模拟农村生活污水,通过检测反应器进出水中各污染物的浓度变化特性,确定MBBR挂膜条件及对污水的处理效果.研究表明,启动期活性污泥产生的黏性膨胀对挂膜有较大的影响,通过调节ρ(C)∶ρ(N)∶ρ(P)=100∶5∶1,可以改善此现象;MBBR运行稳定后,进水ρ(COD)、ρ(NH4+-N)、ρ(TP)分别为400,35,7 mg/L时,在pH约为7、ρ(DO)为2-4 mg/L、HRT为3h的条件下,COD、NH4+-N 、TN、TP的去除率分别达到85%、85%、60%、70%.MBBR对农村面源污水有较好的处理效果,适合于处理污水管网系统落后的地区和乡镇的面源污水.

  3. 生物滞留技术控制城市面源污染的作用与机理%EFFECT AND MECHANISM OF BIORETENTION TECHNOLOGY FOR URBAN NON-POINT SOURCE POLLUTIONS CONTROL

    Institute of Scientific and Technical Information of China (English)

    李平; 王晟

    2014-01-01

    生物滞留设施可以有效削减降雨径流,并通过过滤、吸附和生物降解去除雨水径流水中的氮、磷、多环芳烃、油脂、重金属、细菌和病毒等,其主要设计参数是:暴雨重现期、面积、稳定下渗率、下凹深度和淹水时间.建议改造城市附属绿地为生物滞留,加快该技术在我国的推广应用.

  4. Predicting precipitation on nonpoint source pollutant exports in the source area of the Liao River, China.

    Science.gov (United States)

    Wang, Y; Bian, J M; Wang, S N; Nie, S Y

    The source area of the Liao River is an important grain growing area in China which experiences serious problems with agricultural nonpoint source pollution (NPS) which is impacting the regional economy and society. In order to address the water quality issues it is necessary to understand the spatial distribution of NPS in the Liao River source area. This issue has been investigated by coupling a wavelet artificial neural network (WA-ANN) precipitation model with a soil and water assessment tool (SWAT) model to assess the export of nonpoint source pollutants from the Liao River source area. The calibration and validation of these models are outlined. The WA-ANN models and the SWAT model were run to generate the spatial distribution of nonpoint source nutrient (nitrogen and phosphorus) exports in the source area of the Liao River. It was found that the SWAT model identified the sub-catchments which not only receive high rainfall but are also densely populated with high agricultural production from dry fields and paddy fields, which are large users of pesticides and chemical fertilizer, as the primary source areas for nutrient exports. It is also concluded that the coupled WA-ANN models and the SWAT model provide a tool which will inform the identification of NPS issues and will facilitate the identification of management practices to improve the water environments in the source area of the Liao River.

  5. Asymmetrical flow field-flow fractionation coupled to inductively coupled plasma mass spectrometry for sizing SeNPs for packaging applications

    Science.gov (United States)

    Palomo-Siguero, María; Vera, Paula; Echegoyen, Yolanda; Nerin, Cristina; Cámara, Carmen; Madrid, Yolanda

    2017-06-01

    This paper describes the application of Asymmetrical Flow Field-Flow Fractionation (AF4) coupled to diode array detector (DAD) and inductively coupled plasma mass spectrometry (AF4-UV-ICP-MS) to characterize selenium nanoparticles (SeNPs) in an aqueous acrylic adhesive to be used in a multilayer food packaging material. SeNPs were synthesized using a solution-phase approach based on the reduction of selenite with ascorbic acid in presence of different stabilizers compatible with food industry such as polysaccharides (chitosan (poly(D-glucosamine) and hydroxyethylcellulose (HEC)) and non-ionic surfactants (Triton X-100 (t-octylphenoxypolyethoxyethanol), 2,4,7,9-tetramethyl 5decyne-4,7-diol ethoxylate, and isotridecanol ethoxylate). Several parameters such as pH, ascorbic acid and stabilizers concentration, and compatibility of the stabilizer with the adhesive were evaluated. SeNPs suspensions with spherical morphology were obtained except when isotridecanol ethoxylate was employed which provides SeNPs with a nanorod morphology. AF4-DAD-ICP-MS was further applied for sizing the different SeNPs preparations. DAD was used as detector for selecting the best AF4 separation conditions before coupling to ICP-MS to ensure unequivocal identification of NPs. AF4 calibration with polystyrene latex (PSL) beads of known sizes allowed size determination of the different SeNPs. The following estimated hydrodynamic sizes (expressed as the mean ± standard deviation, n = 6 replicates) were found: chitosan-SeNPs- (26 ± 3 nm), TritonX100-SeNPs (22 ± 10 nm) HEC- SeNPs (91 ± 8 nm) and 2,4,7,9-tetramethyl 5decyne-4,7-diol ethoxylate- SeNPs (59 ± 4 nm). The proposed methodology was successfully applied to the characterization in terms of size of aqueous acrylic adhesives containing SeNPs Results from AF4-ICP-MS and TEM shown that only those SeNPs obtained with non-ionic surfactants and HEC were compatible with the adhesive. The results reported here evidence the usefulness of AF4-ICP

  6. Mitigation of nonpoint source pollution in rural areas: From control to synergies of multi ecosystem services.

    Science.gov (United States)

    Wu, Yonghong; Liu, Junzhuo; Shen, Renfang; Fu, Bojie

    2017-12-31

    Nonpoint source (NPS) pollution produced by human activities in rural areas has induced excessive nutrient input into surface waters and the decline of water quality. The essence of NPS pollution is the transport of nutrients between soil and water. Traditional NPS pollution control strategies, however, are mainly based on the solid and liquid phases, with little focus on the bio-phase between water and soil. The pollutants produced from NPS can be regarded as a resource if recycled or reused in an appropriate way in the agricultural ecosystem. This mini review proposes novel strategies for NPS pollution control based on three phases (liquid, solid and bio-phase) and highlights the regulating services of an agricultural ecosystem by optimizing land use/cover types. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Fabrication of ITO-rGO/Ag NPs nanocomposite by two-step chronoamperometry electrodeposition and its characterization as SERS substrate

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Rong [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Analytical and Testing Center, Sichuan University of Science & Engineering, Zigong, Sichuan 643000 (China); Xu, Yi [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Wang, Chunyan [Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); School of Optoelectronic Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Zhao, Huazhou; Wang, Renjie; Liao, Xin [Chemistry and Chemical Engineering College, Chongqing University, Shapingba, Chongqing 400044 (China); Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); Chen, Li; Chen, Gang [Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing 400044 (China); School of Optoelectronic Engineering, Chongqing University, Shapingba, Chongqing 400044 (China)

    2015-09-15

    Highlights: • A novel structure of ITO-rGO/Ag NPs substrate was developed for SERS application. • Two-step chronoamperometry deposition method was used to prepare SERS substrate. • The SERS substrate had high SERS activity, good uniformity and reproducibility. - Abstract: A novel composite structure of reduced graphene oxide (rGO)–Ag nanoparticles (Ag NPs) nanocomposite, which was integrated on the indium tin oxide (ITO) glass by a facile and rapid two-step chronoamperometry electrodeposition route, was proposed and developed in this paper. SERS-activity of the rGO/Ag NPs nanocomposite was mainly affected by the structure and size of the fabricated rGO/Ag NPs nanocomposite. In the experiments, the operational conditions of electrodeposition process were studied in details. The electrodeposited time was the important controllable factor, which decided the particle size and surface coverage of the deposited Ag NPs on ITO glass. Under the optimized conditions, the detection limit for rhodamine6G (R6G) was as low as 10{sup −11} M and the Raman enhancement factor was as large as 5.9 × 10{sup 8}, which was 24 times higher than that for the ITO–Ag NPs substrate. Apart from this higher enhancement effect, it was also illustrated that extremely good uniformity and reproducibility with low standard deviation could be obtained by the prepared ITO-rGO/Ag NPs nanocomposite for SRES detection.

  8. An investigation on cytotoxic effect of bioactive AgNPs synthesized using Cassia fistula flower extract on breast cancer cell MCF-7

    Directory of Open Access Journals (Sweden)

    R.R. Remya

    2015-12-01

    Full Text Available A single step protocol to produce biofunctionalized silver nanoparticles (AgNPs using the aqueous extract of Cassia fistula flower as “natural factory” was investigated. The reaction between silver ions and aqueous flower extract after the bioreduction process has resulted in the formation of reddish brown color colloidal solution. XRD pattern showed the face centered cubic crystalline structure of AgNPs and exhibited spherical morphology as characterized by FE-SEM. FTIR studies identified different functional groups involved in effective capping of AgNPs. The zeta potential affirmed the phytoreduced AgNPs possess good stability and the size of the particle was measured by DLS. The synthesized AgNPs displayed effective cytotoxic potential against MCF7 and the inhibitory concentration (IC50 was recorded at 7.19 μg/mL. The apoptotic effects of the AgNPs were also confirmed by AO/EB staining. The investigation presents preliminary evidence that biosynthesized AgNPs can be used in the development of novel anticancer drugs.

  9. Synthesis of metal oxide nanoparticles (CuO and ZnO NPs) via biological template and their optical sensor applications

    Science.gov (United States)

    Maruthupandy, Muthuchamy; Zuo, Yong; Chen, Jing-Shuai; Song, Ji-Ming; Niu, He-Lin; Mao, Chang-Jie; Zhang, Sheng-Yi; Shen, Yu-Hua

    2017-03-01

    The present study is focused on employing Camellia japonica leaf extract as inductive and stabilizing agent to synthesis CuO and ZnO nanoparticles (NPs). The chemicals, such as (Cu(NO3)2·3H2O) and (Zn(NO3)2·6H2O) were converted into copper and zinc ions, respectively because of the different natural products present in the C. japonica leaf extract. The UV-vis spectra of CuO and ZnO NPs showed absorption peak at 290 nm and 301 nm, respectively. The XRD result revealed crystalline nature of the metal oxide NPs and the TEM images indicated that average sizes of the synthesized CuO and ZnO NPs were ∼17 nm and ∼20 nm, respectively. The FTIR spectra of C. japonica leaf extract showed the presence of organic groups, such as, sbnd OH, sbnd Csbnd N, and N-H, which would be responsible for forming CuO and ZnO NPs. The synthesized CuO and ZnO NPs were tested for the optical sensing of metal ions, viz. Li+ and Ag+ that illustrated excellent outcome and hence this method offers a novel lane for the synthesis of metal oxide NPs, which can be used as optical sensor for the detection of metal ions.

  10. Modification-free and N-acetyl-L-cysteine-induced colorimetric response of AuNPs: A mechanistic study and sensitive Hg(2+) detection.

    Science.gov (United States)

    Tang, Jie; Wu, Peng; Hou, Xiandeng; Xu, Kailai

    2016-10-01

    A facile yet sensitive and selective method was proposed for Hg(2+) detection based on N-acetyl-L-cysteine(NAC)-induced colorimetric response of AuNPs. The proposed method can be easily performed by introducing the premixing of NAC and Hg(2+) into as-prepared citrate-capped AuNPs solution. A combination of experimental and theoretical studies was applied to illustrate the mechanism of this AuNPs colorimetric system. The strong interaction of NAC and AuNPs through Au-S bond could lead to the aggregation of AuNPs, but the formation of NAC-Hg-NAC complex decreased the affinity between NAC and AuNPs and resulted in an anti-aggregation effect. Therefore, the color of the AuNPs solution would progress from purple to red with the increase of Hg(2+) concentration. The proposed method had a high sensitivity with a limit of detection of 9.9nM. Coexistent metal ions, including Cd(2+), Mn(2+), Al(3+), Ag(+), K(+), Mg(2+), Ca(2+), Cr(3+), Cu(2+), Fe(3+), Pb(2+), Ni(2+) and Zn(2+), did not interfere with the detection of Hg(2+). This method can be used to monitor Hg(2+) in tap water.

  11. Green synthesis, characterisation and biological evaluation of AgNPs using Agave americana, Mentha spicata and Mangifera indica aqueous leaves extract.

    Science.gov (United States)

    Ahmad, Bashir; Shireen, Farah; Bashir, Shumaila; Khan, Ibrar; Azam, Sadiq

    2016-10-01

    The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) of Agave americana, Mentha spicata and Mangifera indica leaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano-particles was confirmed by transmission electron microscopy (30-150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds. A. americana AgNPs showed significant (96%) activity against Methicillin resistant Staphylococcus aureus, Escherichia coli (95%) and Fusarium oxysporum (89%). Good antioxidant activity was shown by M. spicata AgNPs at 300 µl (79%). M. indica AgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.

  12. Evaluation of environmental safety concentrations of DMSA Coated Fe2O3-NPs using different assay systems in nematode Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Qiuli Wu

    Full Text Available Dimercaptosuccinic acid (DMSA coating improves the uptake efficiency presumably by engendering the Fe(2O(3-NPs. In the present study, we investigated the possible environmental safety concentrations of Fe(2O(3-NPs using different assay systems in nematode Caenorhabditis elegans with lethality, development, reproduction, locomotion behavior, pharyngeal pumping, defecation, intestinal autofluorescence and reactive oxygen species (ROS production as the endpoints. After exposure from L4-larvae for 24-hr, DMSA coated Fe(2O(3-NPs at concentrations more than 50 mg/L exhibited adverse effects on nematodes. After exposure from L1-larvae to adult, DMSA coated Fe(2O(3-NPs at concentrations more than 500 μg/L had adverse effects on nematodes. After exposure from L1-larvae to day-8 adult, DMSA coated Fe(2O(3-NPs at concentrations more than 100 μg/L resulted in the adverse effects on nematodes. Accompanied with the alterations of locomotion behaviors, ROS production was pronouncedly induced by exposure to DMSA coated Fe(2O(3-NPs in the examined three assay systems, and the close associations of ROS production with lethality, growth, reproduction, locomotion behavior, pharyngeal pumping, defecation, or intestinal autofluorescence in nematodes exposed to DMSA coated Fe(2O(3-NPs were confirmed by the linear regression analysis. Moreover, mutations of sod-2 and sod-3 genes, encoding Mn-SODs, showed more susceptible properties than wild-type when they were used for assessing the DMSA coated Fe(2O(3-NPs-induced toxicity, and the safety concentrations for DMSA coated Fe(2O(3-NPs should be defined as concentrations lower than 10 μg/L in sod-2 and sod-3 mutant nematodes.

  13. Formyl-peptide Receptor Agonists and Amorphous SiO2-NPs Synergistically and Selectively Increase the Inflammatory Responses of Human Monocytes and PMNs

    Directory of Open Access Journals (Sweden)

    Regina Tavano

    2016-02-01

    ligands, but not toward FPR2-specific ones. Conversely, the chemotaxis of monocytes toward FPR2-specific peptides was inhibited by SiO2-NPs. NADPH-oxidase activation triggered by FPR1- and FPR2-specific ligands in both cell types was not altered by SiO2-NPs. Microbial and tissue danger signals sensed by FPRs selectively amplified the functional responses of monocytes and PMNS to SiO2-NPs, and should be carefully considered in the assessment of the risk associated with nanoparticle exposure.

  14. Integrated mRNA and micro RNA profiling reveals epigenetic mechanism of differential sensitivity of Jurkat T cells to AgNPs and Ag ions.

    Science.gov (United States)

    Eom, Hyun-Jeong; Chatterjee, Nivedita; Lee, Jeongsoo; Choi, Jinhee

    2014-08-17

    In our previous in vitro study of the toxicity on silver nanoparticles (AgNPs), we observed a dramatically higher sensitivity of Jurkat T cells to AgNPs than to Ag ions, and DNA damage and apoptosis were found to be involved in that toxicity. In this study, to understand underlying mechanism of different sensitivity of Jurket T cells to AgNPs and Ag ions, mRNA microarray and micro RNA microarray were concomitantly conducted on AgNPs and Ag ions exposed Jurkat T cells. Surprisingly only a small number of genes were differentially expressed by exposure to each of the silver (15 altered mRNA by AgNPs exposure, whereas 4 altered mRNA by Ag ions exposure, as determined 1.5-fold change as the cut-off value). miRNA microarray revealed that the expression of 63 miRNAs was altered by AgNPs exposure, whereas that of 32 miRNAs was altered by Ag ions exposure. An integrated analysis of mRNA and miRNA expression revealed that the expression of hsa-miR-219-5p, was negatively correlated with the expression of metallothionein 1F (MT1F) and tribbles homolog 3 (TRIB3), in cells exposed to AgNPs; whereas, the expression of hsa-miR-654-3p was negatively correlated with the expression of mRNA, endonuclease G-like 1 (EDGL1) in cells exposed to Ag ions. Network analysis were further conducted on mRNA-miRNA pairs, which revealed that miR-219-5p-MT1F and -TRIB3 pairs by AgNPs are being involved in various cellular processes, such as, oxidative stress, cell cycle and apoptosis, whereas, miR-654-3p and ENDOGL1 pair by Ag ions generated a much simpler network. The putative target genes of AgNPs-induced miR-504, miR-33 and miR-302 identified by Tarbase 6.0 are also found to be involved in DNA damage and apoptosis. These results collectively suggest that distinct epigenetic regulation may be an underlying mechanism of different sensitivity of Jurkat T cells to AgNPs and Ag ion. Further identification of putative target genes of DE miRNA by AgNPs and Ag ions may provide additional clues for the

  15. ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor

    Science.gov (United States)

    Arciniegas-Grijalba, P. A.; Patiño-Portela, M. C.; Mosquera-Sánchez, L. P.; Guerrero-Vargas, J. A.; Rodríguez-Páez, J. E.

    2017-06-01

    In this work, a methodology of synthesis was designed to obtain ZnO nanoparticles (ZnO NPs) in a controlled and reproducible manner. The nanoparticles obtained were characterized using infrared spectroscopy, X-ray diffraction, and transmission electron microscopy (TEM). Also, we determined the antifungal capacity in vitro of zinc oxide nanoparticles synthesized, examining their action on Erythricium salmonicolor fungy causal of pink disease. To determine the effect of the quantity of zinc precursor used during ZnO NPs synthesis on the antifungal capacity, 0.1 and 0.15 M concentrations of zinc acetate were examined. To study the inactivation of the mycelial growth of the fungus, different concentrations of ZnO NPs of the two types of synthesized samples were used. The inhibitory effect on the growth of the fungus was determined by measuring the growth area as a function of time. The morphological change was observed with high-resolution optical microscopy (HROM), while TEM was used to observe changes in its ultrastructure. The results showed that a concentration of 9 mmol L-1 for the sample obtained from the 0.15 M and at 12 mmol L-1 for the 0.1 M system significantly inhibited growth of E. salmonicolor. In the HROM images a deformation was observed in the growth pattern: notable thinning of the fibers of the hyphae and a clumping tendency. The TEM images showed a liquefaction of the cytoplasmic content, making it less electron-dense, with the presence of a number of vacuoles and significant detachment of the cell wall.

  16. Two 24-hour Studies of Water Quality in the Ala Wai Canal during March and July, 1994 for the Mamala Bay Study, Pollutant Source Identification Project MB-3, (NODC Accession 0001188)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset focuses on studies of water quality in the Ala Wai Canal in order to determine its role of point and non point source disharge into Mamala Bay. The...

  17. Enhancement of TiO2 NPs Activity by Fe3O4 Nano-Seeds for Removal of Organic Pollutants in Water

    National Research Council Canada - National Science Library

    Villa, Silvia; Caratto, Valentina; Locardi, Federico; Alberti, Stefano; Sturini, Michela; Speltini, Andrea; Maraschi, Federica; Canepa, Fabio; Ferretti, Maurizio

    2016-01-01

    .... Photocatalytic activity data, i.e., the degradation of methylene blue and the Ofloxacin fluoroquinolone emerging pollutant, give evidence that the increased crystalline structure of the NPs, even if correlated to a reduced surface to mass ratio...

  18. Electrochemiluminescence induced photoelectrochemistry for sensing of the DNA based on DNA-linked CdS NPs superstructure with intercalator molecules.

    Science.gov (United States)

    Guo, Yingshu; Sun, Yuanshun; Zhang, Shusheng

    2011-02-07

    A novel detection protocol of DNA was developed using electrochemiluminescence (ECL) induced photoelectrochemistry (PEC) synthesis based on DNA-linked CdS NPs superstructure with methylene blue as the intercalator molecule.

  19. Bathymetry 2M Grid, of NPS's Virgin Islands Coral Reef National Monument (Offshore), St. John, US Virgin Islands, 2005, UTM 20 NAD83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Grid with 2 meter cell size representing the bathymetry of an offshore portion of the NPS's Virgin Islands Coral Reef National...

  20. Bathymetry 2M Grid of NPS's Salt River Bay National Historical Park and Ecological Reserve, St. Croix, US Virgin Islands, 2005, UTM 20 NAD83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Grid with 2 meter cell size representing the bathymetry of the a portion of the NPS's Salt River Bay National Historical Park and...

  1. Bathymetry of NPS's Virgin Islands Coral Reef National Monument (Inshore), St. John, US Virgin Islands 2005, 1M Grid, UTM 20 NAD83

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an ESRI Grid with 1 meter cell size representing the bathymetry of an inshore portion of the NPS's Virgin Islands Coral Reef National Monument,...

  2. Neurite outgrowth stimulatory effects of myco synthesized AuNPs from Hericium erinaceus (Bull.: Fr.) Pers. on pheochromocytoma (PC-12) cells.

    Science.gov (United States)

    Raman, Jegadeesh; Lakshmanan, Hariprasath; John, Priscilla A; Zhijian, Chan; Periasamy, Vengadesh; David, Pamela; Naidu, Murali; Sabaratnam, Vikineswary

    2015-01-01

    Hericium erinaceus has been reported to have a wide range of medicinal properties such as stimulation of neurite outgrowth, promotion of functional recovery of axonotmetic peroneal nerve injury, antioxidant, antihypertensive, and antidiabetic properties. In recent years, the green synthesis of gold nanoparticles (AuNPs) has attracted intense interest due to the potential use in biomedical applications. The aim of this study was to investigate the effects of AuNPs from aqueous extract of H. erinaceus on neurite outgrowth of rat pheochromocytoma (PC-12) cells. The formation of AuNPs was characterized by UV-visible spectrum, energy dispersive X-ray (EDX), field-emission scanning electron microscope (FESEM), transmission electron microscopy (TEM), particle size distribution, and Fourier transform-infrared spectroscopy (FTIR). Furthermore, the neurite extension study of synthesized AuNPs was evaluated by in vitro assay. The AuNPs exhibited maximum absorbance between 510 and 600 nm in UV-visible spectrum. FESEM and TEM images showed the existence of nanoparticles with sizes of 20-40 nm. FTIR measurements were carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the nanoparticles. The purity and the crystalline properties were confirmed by EDX diffraction analysis, which showed strong signals with energy peaks in the range of 2-2.4 keV, indicating the existence of gold atoms. The synthesized AuNPs showed significant neurite extension on PC-12 cells. Nerve growth factor 50 ng/mL was used as a positive control. Treatment with different concentrations (nanograms) of AuNPs resulted in neuronal differentiation and neuronal elongation. AuNPs induced maximum neurite outgrowth of 13% at 600 ng/mL concentration. In this study, the AuNPs synthesis was achieved by a simple, low-cost, and rapid bioreduction approach. AuNPs were shown to have potential neuronal differentiation and stimulated neurite outgrowth. The water

  3. Cytotoxicity of ZnO NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C, visible and dark conditions

    Energy Technology Data Exchange (ETDEWEB)

    Bhuvaneshwari, M.; Iswarya, V. [Centre for Nanobiotechnology, VIT University, Vellore 632014 (India); Archanaa, S. [Department of Biotechnology, IIT Madras (India); Madhu, G.M. [Department of Chemical Engineering, M.S. Ramaiah Institute of Technology, Bangalore (India); Kumar, G.K. Suraish [Department of Biotechnology, IIT Madras (India); Nagarajan, R. [Department of Chemical Engineering, IIT Madras (India); Chandrasekaran, N. [Centre for Nanobiotechnology, VIT University, Vellore 632014 (India); Mukherjee, Amitava, E-mail: amit.mookerjea@gmail.com [Centre for Nanobiotechnology, VIT University, Vellore 632014 (India)

    2015-05-15

    Highlights: • The cytotoxicity of ZnO NPs with two hydrodynamic sizes towards freshwater algae. • Size dependent toxicity under UV-C, dark and visible light conditions. • Cytotoxicity principally due to ZnO NPs not the released Zn{sup 2+} ions. • The internalization of ZnO NPs leads to membrane damage and ROS production. - Abstract: Continuous increase in the usage of ZnO nanoparticles in commercial products has exacerbated the risk of release of these particles into the aquatic environment with possible harmful effects on the biota. In the current study, cytotoxic effects of two types of ZnO nanoparticles, having different initial effective diameters in filtered and sterilized lake water medium [487.5 ± 2.55 nm for ZnO-1 NPs and 616.2 ± 38.5 nm for ZnO-2 NPs] were evaluated towards a dominant freshwater algal isolate Scenedesmus obliquus in UV-C, visible and dark conditions at three exposure concentrations: 0.25, 0.5 and 1 mg/L. The toxic effects were found to be strongly dependent on the initial hydrodynamic particle size in the medium, the exposure concentrations and the irradiation conditions. The loss in viability, LDH release and ROS generation were significantly enhanced in the case of the smaller sized ZnO-1 NPs than in the case of ZnO-2 NPs under comparable test conditions. The toxicity of both types of ZnO NPs was considerably elevated under UV-C irradiation in comparison to that in dark and visible light conditions, the effects being more enhanced in case of ZnO-1 NPs. The size dependent dissolution of the ZnO NPs in the test medium and possible toxicity due to the released Zn{sup 2+} ions was also noted. The surface adsorption of the nanoparticles was substantiated by scanning electron microscopy. The internalization/uptake of the NPs by the algal cells was confirmed by fluorescence microscopy, transmission electron microscopy, and elemental analyses.

  4. Enhanced corrosion resistance and biocompatibility of AZ31 Mg alloy using PCL/ZnO NPs via electrospinning

    Science.gov (United States)

    Kim, Jinwoo; Mousa, Hamouda M.; Park, Chan Hee; Kim, Cheol Sang

    2017-02-01

    In the efforts to improve corrosion resistance and biocompatibility of magnesium alloys, polycarprolactone (PCL) and zinc oxide nanoparticles (ZnO NPs) composite coatings were applied onto AZ31 Mg alloys via electrospinning technique in this study. The PCL/ZnO composite coatings on Mg alloys were characterized by using FE-SEM, EDX, XPS, and FT-IR. Moreover, coating adhesion test, electrochemical corrosion test, and biocompatibility test in vitro were performed to measure coating performance. Our results revealed that the increase in the content of ZnO NPs in the composite coatings not only improved the coating adhesion of composite coatings on Mg alloys, but also increased the corrosion resistance. Furthermore, the biocompatibility of MC3T3-E1 osteoblasts of the PCL/ZnO composite coated samples was superior to the biocompatibility of the bare samples. Such data suggest that applying PCL/ZnO composite coating to the magnesium alloys has suitable potential in biomedical applications.

  5. DETERMINING THE ACUTE TOXIC EFFECTS OF POLY(VINYLFERROCENIUM SUPPORTED PLATINUM NANOPARTICLE (PT/PVF+ NPS ON APIS MELLIFERA

    Directory of Open Access Journals (Sweden)

    Yeşim Dağlıoğlu

    2016-12-01

    Full Text Available The use of engineering nanomaterials on a large scale along with their production, and their potential effects on the environment and on human health as well as their environmental emission have increased these concerns. For this reason, nanoparticles which are released into the environment are necessary determine the toxicity by using indicator organisms. With this study, it was aimed that the acute toxic effects of Polyvinylferrocene (PVF+- supported platinum (Pt nanoparticle (Pt/PVF+ NPs, Poly(vinylferrocenium (PVF+ and K2PtCl4 be evaluted comparatively by using the honey bees (Apis Mellifera. LC50 values for 48 and 96 hours of these substances respectively; 713.290 ve 6.899 mg/l for K2PtCl4; 12458374.000 ve 178.262 mg/l for Pt/PVF+ NPs and 148.153 ve 0.344 mg/l for PVF+. When we look at this value, the toxic effect for all three substance had increased on a serious level, depending on the exposure time.

  6. Effects of reducibility of graphene oxide nanosheets on preparation of AgNPs/GO nanocomposites and their electrocatalytic performance

    Energy Technology Data Exchange (ETDEWEB)

    Dou, Yanpeng; Peng, Junjun, E-mail: john-peng@whu.edu.cn; Li, Wei; Li, Ming; Liu, Huihong; Zhang, Hanmin [Wuhan Textile University, College of Chemistry and Chemical Engineering (China)

    2015-12-15

    Silver nanoparticles/graphene oxide (AgNPs/GO) nanocomposites were prepared in a solution of AgNO{sub 3} and GO. The GO serves not only as a reductant but also as a substrate to support the as-reduced silver nanoparticles. The reducibility of GO was investigated by analyzing the influence factors such as pH, duration, the reaction temperature, and the weight ratio of AgNO{sub 3} and GO in the AgNP/GO nanocomposite mixture, which were evaluated by the UV–vis absorption spectroscopy. The results demonstrated that Ag nanoparticles with an average diameter of 5–10 nm were uniformly dispersed on the surface of GO nanosheets under the optimum synthesis conditions of pH between 8 and 11, weight ratio of AgNO{sub 3} and GO between 55 % and 60 %, and at 80 °C for 6 h. Moreover, the obtained AgNPs/GO nanocomposites exhibit good electrocatalytic activity for the reduction of p-nitrophenol to 4-(hydroxyamino) phenol.

  7. Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate

    Directory of Open Access Journals (Sweden)

    Xiangyou Wang

    2013-04-01

    Full Text Available In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au nanocomposites were modified onto the surface of a glass carbon electrode (GCE via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD, ultraviolet and visible absorption spectroscopy (UV-vis and scanning electron microscopy (SEM, respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3. The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication.

  8. Amperometric immunosensor for carbofuran detection based on MWCNTs/GS-PEI-Au and AuNPs-antibody conjugate.

    Science.gov (United States)

    Zhu, Ying; Cao, Yaoyao; Sun, Xia; Wang, Xiangyou

    2013-04-19

    In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs) and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au) nanocomposites were modified onto the surface of a glass carbon electrode (GCE) via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab) and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD), ultraviolet and visible absorption spectroscopy (UV-vis) and scanning electron microscopy (SEM), respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3). The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication.

  9. Cytotoxicity of ZnO NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C, visible and dark conditions.

    Science.gov (United States)

    Bhuvaneshwari, M; Iswarya, V; Archanaa, S; Madhu, G M; Kumar, G K Suraish; Nagarajan, R; Chandrasekaran, N; Mukherjee, Amitava

    2015-05-01

    Continuous increase in the usage of ZnO nanoparticles in commercial products has exacerbated the risk of release of these particles into the aquatic environment with possible harmful effects on the biota. In the current study, cytotoxic effects of two types of ZnO nanoparticles, having different initial effective diameters in filtered and sterilized lake water medium [487.5±2.55 nm for ZnO-1 NPs and 616.2±38.5 nm for ZnO-2 NPs] were evaluated towards a dominant freshwater algal isolate Scenedesmus obliquus in UV-C, visible and dark conditions at three exposure concentrations: 0.25, 0.5 and 1 mg/L. The toxic effects were found to be strongly dependent on the initial hydrodynamic particle size in the medium, the exposure concentrations and the irradiation conditions. The loss in viability, LDH release and ROS generation were significantly enhanced in the case of the smaller sized ZnO-1 NPs than in the case of ZnO-2 NPs under comparable test conditions. The toxicity of both types of ZnO NPs was considerably elevated under UV-C irradiation in comparison to that in dark and visible light conditions, the effects being more enhanced in case of ZnO-1 NPs. The size dependent dissolution of the ZnO NPs in the test medium and possible toxicity due to the released Zn(2+) ions was also noted. The surface adsorption of the nanoparticles was substantiated by scanning electron microscopy. The internalization/uptake of the NPs by the algal cells was confirmed by fluorescence microscopy, transmission electron microscopy, and elemental analyses.

  10. Nano-pulse stimulation (NPS) ablate tumors and inhibit lung metastasis on both canine spontaneous osteosarcoma and murine transplanted hepatocellular carcinoma with high metastatic potential

    OpenAIRE

    Chen, Xinhua; Chen, Yiling; Jiang, Jianwen; Wu, Liming; Yin, Shengyong; Miao, Xudong; Swanson, Robert J.; Zheng, Shusen

    2017-01-01

    Background Nanosecond pulsed electric field (nsPEF), which is also termed as nano-pulse stimulation (NPS), has the potential of stimulating immune responses toward cancer cells. The current study investigates its local and systemic antitumor efficacy in vivo in late stage tumors with lung metastasis. Method The 12 canines with spontaneous osteosarcomas and 12 nude mice transplanted with human hepatocellular carcinoma were divided randomly and were given NPS treatment, surgery or no treatment ...

  11. Synthesis, characterization and in vitro study of biocompatible cinnamaldehyde functionalized magnetite nanoparticles (CPGF Nps for hyperthermia and drug delivery applications in breast cancer.

    Directory of Open Access Journals (Sweden)

    Kirtee D Wani

    Full Text Available Cinnamaldehyde, the bioactive component of the spice cinnamon, and its derivatives have been shown to possess anti-cancer activity against various cancer cell lines. However, its hydrophobic nature invites attention for efficient drug delivery systems that would enhance the bioavailability of cinnamaldehyde without affecting its bioactivity. Here, we report the synthesis of stable aqueous suspension of cinnamaldehyde tagged Fe3O4 nanoparticles capped with glycine and pluronic polymer (CPGF NPs for their potential application in drug delivery and hyperthermia in breast cancer. The monodispersed superparamagnetic NPs had an average particulate size of ∼ 20 nm. TGA data revealed the drug payload of ∼ 18%. Compared to the free cinnamaldehyde, CPGF NPs reduced the viability of breast cancer cell lines, MCF7 and MDAMB231, at lower doses of cinnamaldehyde suggesting its increased bioavailability and in turn its therapeutic efficacy in the cells. Interestingly, the NPs were non-toxic to the non-cancerous HEK293 and MCF10A cell lines compared to the free cinnamaldehyde. The novelty of CPGF nanoparticulate system was that it could induce cytotoxicity in both ER/PR positive/Her2 negative (MCF7 and ER/PR negative/Her2 negative (MDAMB231 breast cancer cells, the latter being insensitive to most of the chemotherapeutic drugs. The NPs decreased the growth of the breast cancer cells in a dose-dependent manner and altered their migration through reduction in MMP-2 expression. CPGF NPs also decreased the expression of VEGF, an important oncomarker of tumor angiogenesis. They induced apoptosis in breast cancer cells through loss of mitochondrial membrane potential and activation of caspase-3. Interestingly, upon exposure to the radiofrequency waves, the NPs heated up to 41.6 °C within 1 min, suggesting their promise as a magnetic hyperthermia agent. All these findings indicate that CPGF NPs prove to be potential nano-chemotherapeutic agents in breast cancer.

  12. Climate Change Education in Protected Areas: Highlights from the Earth to Sky NASA-NPS-USFWS Partnership

    Science.gov (United States)

    Davis, A.; Morris, J.; Paglierani, R.

    2009-12-01

    National Parks, Hatcheries, Refuges, and other protected lands provide ideal settings for communicating the immediate and obvious effects of climate change, from rapidly melting glaciers, increased intensity and length of fire seasons, to flooding of archeological and historical treasures. Our nation's protected areas demonstrate clearly that climate change is happening now, and the impacts are affecting us all. Highlights of interpretive, educational and informational products presented in these sites, and developed through the Earth to Sky (ETS) partnership are described. The visiting public in our nation's parks, refuges, cultural sites and other protected lands wants to learn more about climate change, and is asking questions—often, complex questions. A broad array of educational programs and media are delivered in these unique settings, to diverse audiences. To be good "honest brokers" of the best information, staff needs access to accurate, up-to-date data, descriptions, analysis, and imagery that make the issues understandable. Pairing real world experiences of climate effects such as glacial retreat or beetle infestations, with NASA’s unique planetary perspective provides opportunities to link local, regional, and global effects in the minds and hearts of the public and students. The perspective afforded by such linkages can create powerful and long lasting impressions, and will likely provoke further learning about this topic. About Earth to Sky Earth to Sky is a partnership between NASA's Space and Earth Science disciplines, the US Fish and Wildlife Service (USFWS), and the National Park Service (NPS). The partnership actively fosters collaborative work between the science and interpretation/education communities of NPS, USFWS, and NASA, centering around a series of professional development workshops aimed at informal educators. The workshops weave NASA content with NPS and USFWS interpretation and environmental education methodology, and use best

  13. Biogenic SeNPs from Bacillus mycoides SelTE01 and Stenotrophomonas maltophilia SelTE02: Characterization with reference to their associated organic coating

    Science.gov (United States)

    Piacenza, Elena; Bulgarini, Alessandra; Lampis, Silvia; Vallini, Giovanni; Turner, Raymond J.

    2017-08-01

    The exploitation of biological systems (i.e. plants, fungi and bacteria) for the production of nanomaterials relies on their ability to bioconvert toxic metal(loid) ions into their less toxic and bioavailable elemental states forming mainly nanoparticles (NPs) or nanorods (NRs). Further, these methods of nanomaterial production are nowadays recognized as eco-friendly alternatives to the chemical synthesis processes. A common feature among the so-called biogenic nanomaterials is the presence of an organic layer surrounding them. However, we are just learning the existing relation between biogenic nanostructures and their organic material. Our work is focused on the study of bacterial strains for the production of selenium nanoparticles (SeNPs) as end product of selenite (SeO32 -) bioconversion. In this context, our previous reports described the ability of two bacteria, namely Bacillus mycoides SelTE01 and Stenotrophomonas maltophilia SelTE02, to generate SeNPs, which were surrounded by organic material. Here, the potential role of this organic material as stabilizing agent of SeNPs was investigated altering both the bacteria cells culturing and the SeNPs extraction procedure, in order to understand the interaction between these two elements in suspension. As a result, SeNPs produced by both bacterial strains showed the tendency to aggregate when subjected to the treatments tested, suggesting an involvement of the surrounding organic material in their stabilization in suspension.

  14. Synthesis, kinetics and photocatalytic study of "ultra-small" Ag-NPs obtained by a green chemistry method using an extract of Rosa 'Andeli' double delight petals.

    Science.gov (United States)

    Suárez-Cerda, Javier; Alonso-Nuñez, Gabriel; Espinoza-Gómez, Heriberto; Flores-López, Lucía Z

    2015-11-15

    This paper reports the effect of different concentrations of Rosa 'Andeli' double delight petals aqueous extract (PERA) in the synthesis of silver nanoparticles (Ag-NPs), using an easy green chemistry method. Its kinetics study and photocatalytic activity were also evaluated. The Ag-NPs were obtained using an aqueous silver nitrate solution (AgNO3) with 9.66% w/v, 7.25% w/v, and 4.20% w/v PERA as both reducing-stabilizing agent. The formation of the Ag-NPs was demonstrated by analysis of UV-vis spectroscopy, scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and transmission electron microscopy (TEM). TEM analysis shows spherical nanoparticles in shape and size between ∼0.5 and 1.4nm. A comparative study was done to determine which concentration was the best reducing-stabilizing agent, and we found out that "ultra-small" nanoparticles (0.5-1.1nm) were obtained with 9.66% w/v of PERA. The size of the Ag-NPs depends on the concentration of PERA and Ag(I). The reaction of formation of "ultra-small" Ag-NPs, proved to be first order for metallic precursor (silver) and second order for reducing-stabilizing agent (PERA). The Ag-NPs showed photocatalytic activity, in degradation of commercial dye with an efficiency of 95%.

  15. A simple electrochemical biosensor based on AuNPs/MPS/Au electrode sensing layer for monitoring carbamate pesticides in real samples.

    Science.gov (United States)

    Song, Yonghai; Chen, Jingyi; Sun, Min; Gong, Coucong; Shen, Yuan; Song, Yonggui; Wang, Li

    2016-03-05

    A simple electrochemical biosensor for quantitative determination of carbamate pesticide was developed based on a sensing interface of citrate-capped gold nanoparticles (AuNPs)/(3-mercaptopropyl)-trimethoxysilane (MPS)/gold electrode (Au). The biosensor was fabricated by firstly assembling three-dimensional (3D) MPS networks on Au electrode and subsequently assembling citrate-capped AuNPs on 3D MPS network via AuS bond. The interface of AuNPs/MPS/Au was negatively charged originating from the citrate coated on AuNPs that would repulse the negatively charged ferricyanide ([Fe(CN)6](3-/4-)) to produce a negative response. In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. The resulted positively charged AuNPs/MPS/Au then attracted the [Fe(CN)6](3-/4-) to produce a positive response. Based on the inhibition of carbamate pesticides on the activity of AChE, the pesticide could be quantitatively determined at a very low potential. The linear range was from 0.003 to 2.00 μM. The sensing platform was also proved to be suitable for carbamate pesticides detection in practical sample. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Decorating CdTe QD-Embedded Mesoporous Silica Nanospheres with Ag NPs to Prevent Bacteria Invasion for Enhanced Anticounterfeit Applications.

    Science.gov (United States)

    Gao, Yangyang; Dong, Qigeqi; Lan, Shi; Cai, Qian; Simalou, Oudjaniyobi; Zhang, Shiqi; Gao, Ge; Chokto, Harnoode; Dong, Alideertu

    2015-05-13

    Quantum dots (QDs) as potent candidates possess advantageous superiority in fluorescence imaging applications, but they are susceptible to the biological circumstances (e.g., bacterial environment), leading to fluorescence quenching or lose of fluorescent properties. In this work, CdTe QDs were embedded into mesoporous silica nanospheres (m-SiO2 NSs) for preventing QD agglomeration, and then CdTe QD-embedded m-SiO2 NSs (m-SiO2/CdTe NSs) were modified with Ag nanoparticles (Ag NPs) to prevent bacteria invasion for enhanced anticounterfeit applications. The m-SiO2 NSs, which serve as intermediate layers to combine CdTe QDs with Ag NPs, help us establish a highly fluorescent and long-term antibacterial system (i.e., m-SiO2/CdTe/Ag NSs). More importantly, CdTe QD-embedded m-SiO2 NSs showed fluorescence quenching when they encounter bacteria, which was avoided by attaching Ag NPs outside. Ag NPs are superior to CdTe QDs for preventing bacteria invasion because of the structure (well-dispersed Ag NPs), size (small diameter), and surface charge (positive zeta potentials) of Ag NPs. The plausible antibacterial mechanisms of m-SiO2/CdTe/Ag NSs toward both Gram-positive and Gram-negative bacteria were established. As for potential applications, m-SiO2/CdTe/Ag NSs were developed as fluorescent anticounterfeiting ink for enhanced imaging applications.

  17. Application of Oligonucleotide Microarrays for Bacterial Source Tracking of Environmental Enterococcus sp. Isolates

    OpenAIRE

    Furey, John S.; Kelley Betts; Indest, Karl J.

    2005-01-01

    In an effort towards adapting new and defensible methods for assessing and managing the risk posed by microbial pollution, we evaluated the utility of oligonucleotide microarrays for bacterial source tracking (BST) of environmental Enterococcus sp. isolates derived from various host sources. Current bacterial source tracking approaches rely on various phenotypic and genotypic methods to identify sources of bacterial contamination resulting from point or non-point pollution. For this study Ent...

  18. Green synthesis of anticancerous honeycomb PtNPs clusters: Their alteration effect on BSA and HsDNA using fluorescence probe.

    Science.gov (United States)

    Pansare, Amol V; Kulal, Dnyaneshwar K; Shedge, Amol A; Patil, Vishwanath R

    2016-09-01

    The screening and characterization of cancer cells has been challenging due to sample insufficiency and extravagant. In this article, we highlighted easy green synthesis of Platinum nanoparticles (PtNPs) in the honeycomb like clusters, and their optical properties (by HRTEM, XRD, DLS, Zeta potential, EDAX, and UV-Visible techniques). PtNPs were responsive of binding mechanisms with the bovine serum albumin (BSA), herring sperm deoxyribonucleic acid (HsDNA) and cytotoxicity of human carcinomas cell. We are able to elucidate the responses of various concentrations of PtNPs for the control of MDA-MB-468 cell and binding conformation of BSA and HsDNA by using multi-spectroscopic techniques under the physiological conditions. The extent of quenching was in agreement of PtNPs-BSA binding reaction was mainly a static. The Ksv, K, the number of binding sites at different temperatures and the thermodynamic parameters between BSA and PtNPs were calculated. The positive ΔS(0) and negative ΔH(0), ΔG(0) values indicated that the binding pattern was determined by spontaneous hydrogen bond electrostatic interaction of BSA with esterage like activity. The binding properties of the PtNPs with HsDNA have been investigated by thermal denaturation, competitive DNA-binding studies with ethidium bromide (EB), Hochest-33258 and relative viscosity. The negative ΔH(0), ΔS(0) and ΔG(0) values indicated that the hydrophilic interaction were main force in spontaneity in binding mechanism of PtNPs to HsDNA. GI50 value of PtNPs demonstrated that these nanoparticles showed cytotoxicity against MDA-MB-468 human breast cancer cell line. Our results also clarified that PtNPs bind to BSA and can be effectively transported in the body and eliminated. PtNPs showed minor groove binding with HsDNA, which could be a useful guideline for further versatile approach to develop biomedical coatings with different functions of drug design.

  19. Effect of alginate on the aggregation kinetics of copper oxide nanoparticles (CuO NPs): bridging interaction and hetero-aggregation induced by Ca(2.).

    Science.gov (United States)

    Miao, Lingzhan; Wang, Chao; Hou, Jun; Wang, Peifang; Ao, Yanhui; Li, Yi; Lv, Bowen; Yang, Yangyang; You, Guoxiang; Xu, Yi

    2016-06-01

    The stability of CuO nanoparticles (NPs) is expected to play a key role in the environmental risk assessment of nanotoxicity in aquatic systems. In this study, the effect of alginate (model polysaccharides) on the stability of CuO NPs in various environmentally relevant ionic strength conditions was investigated by using time-resolved dynamic light scattering. Significant aggregation of CuO NPs was observed in the presence of both monovalent and divalent cations. The critical coagulation concentrations (CCC) were 54.5 and 2.9 mM for NaNO3 and Ca(NO3)2, respectively. The presence of alginate slowed nano-CuO aggregation rates over the entire NaNO3 concentration range due to the combined electrostatic and steric effect. High concentrations of Ca(2+) (>6 mM) resulted in stronger adsorption of alginate onto CuO NPs; however, enhanced aggregation of CuO NPs occurred simultaneously under the same conditions. Spectroscopic analysis revealed that the bridging interaction of alginate with Ca(2+) might be an important mechanism for the enhanced aggregation. Furthermore, significant coagulation of the alginate molecules was observed in solutions of high Ca(2+) concentrations, indicating a hetero-aggregation mechanism between the alginate-covered CuO NPs and the unabsorbed alginate. These results suggested a different aggregation mechanism of NPs might co-exist in aqueous systems enriched with natural organic matter, which should be taken into consideration in future studies. Graphical abstract Hetero-aggregation mechanism of CuO nanoparticles and alginate under high concentration of Ca(2.)

  20. Fallout and drinking water contamination by I-131 and Cs-134, 137 in Japan, from the Fukushima Daiichi NPS accident

    Energy Technology Data Exchange (ETDEWEB)

    Kelecom, Alphonse; Miyashita, Erika; Kelecom, Patrick Vicent [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil)

    2011-07-01

    The earthquake followed by a tsunami in Japan, on last March 11, seriously damaged four of the six reactors of the Fukushima Daiichi nuclear power station (NPS). Radioactive smokes and highly contaminated water were released for weeks to the environment. Since March 12, when the plant operator TEPCO and Japan's nuclear agency (NISA) confirmed the presence of radionuclides near the NPS, a giant environmental monitoring operation was set up, covering the entire Japanese territory. Daily thousands measurements are realized. We here analyze data released during 60 days on I-131 and Cs-134,137 radioactive concentrations in drinking water and fallout for 45 prefectures. Miyagi and Fukushima, that requires a separate study, are not considered here. Drinking water contamination by I-131 was observed in 13 prefectures, including Tokyo. The most impacted one was Tochigi (maximum of 110 Bq/l, March 24). This value turned water not drinkable for infants and babies. Cs-137 was detected in drinking water in 8 prefectures, with a maximum level of 18 Bq/l in Ibaraki. These levels do not affect potability of tap water. I-131 was observed in fallout in 27 prefectures, with level reaching 93 kBq/m2 in Ibaraki and 36 kBq/m{sup 2} in Tokyo on March 21 and 23 respectively. Fallout of Cs-137 was observed in 19 prefectures. The maximum deposition occurred again in Ibaraki (13kBq/m{sup 2}, March 21) and in Tokyo (5.3 kBq/m2, March 22). Since mid April, only trace contamination has been observed for both radionuclides in drinking water. Sporadically medium levels of Cs-137 are still observed in fallout. (author)

  1. Zoning of rural water conservation in China: A case study at Ashihe River Basin

    OpenAIRE

    2015-01-01

    With the effective control of point source (PS) pollution accomplished, water pollution problems caused by non-point source (NPS) pollution have increased in recent years. The worsening agricultural NPS pollution has drawn the attention of the Chinese Government and researcher scientists and has resulted in the often mentioned “three red lines” on water resources management. One of the red lines is to control water pollution within a rational range. The Agricultural NPS pollution, which inclu...

  2. Proteomic response of mussels Mytilus galloprovincialis exposed to CuO NPs and Cu²⁺: an exploratory biomarker discovery.

    Science.gov (United States)

    Gomes, Tânia; Chora, Suze; Pereira, Catarina G; Cardoso, Cátia; Bebianno, Maria João

    2014-10-01

    CuO NPs are one of the most used metal nanomaterials nowadays with several industrial and other commercial applications. Nevertheless, less is known about the mechanisms by which these NPs inflict toxicity in mussels and to what extent it differs from Cu(2+). The aim of this study was to investigate changes in protein expression profiles in mussels Mytilus galloprovincialis exposed for 15 days to CuO NPs and Cu(2+) (10 μg L(-1)) using a proteomic approach. Results demonstrate that CuO NPs and Cu(2+) induced major changes in protein expression in mussels' showing several tissue and metal-dependent responses. CuO NPs showed a higher tendency to up-regulate proteins in the gills and down-regulate in the digestive gland, while Cu(2+) showed the opposite tendency. Distinctive sets of differentially expressed proteins were found, either common or specific to each Cu form and tissue, reflecting different mechanisms involved in their toxicity. Fifteen of the differentially expressed proteins from both tissues were identified by MALDI-TOF-TOF. Identified proteins indicate common response mechanisms induced by CuO NPs and Cu(2+), namely in cytoskeleton and cell structure (actin, α-tubulin, paramyosin), stress response (heat shock cognate 71, putative C1q domain containing protein), transcription regulation (zinc-finger BED domain-containing protein 1, nuclear receptor subfamily 1G) and energy metabolism (ATP synthase F0 subunit 6). CuO NPs alone also had a marked effect on other biological processes, namely oxidative stress (GST), proteolysis (cathepsin L) and apoptosis (caspase 3/7-1). On the other hand, Cu(2+) affected a protein associated with adhesion and mobility, precollagen-D that is associated with the detoxification mechanism of Cu(2+). Protein identification clearly showed that the toxicity of CuO NPs is not solely due to Cu(2+) dissolution and can result in mitochondrial and nucleus stress-induced cell signalling cascades that can lead to apoptosis. While the

  3. Effect and Properties of Surface-Modified Copper Doped ZnO Nanoparticles (Cu:ZnO NPs on Killing Curves of Bacterial Pathogens

    Directory of Open Access Journals (Sweden)

    Enayatollah Kalantar

    2015-10-01

    Full Text Available Background: The current study aimed to determine the effect and properties of surface-modified copper doped Cu:ZnO NPs on killing curves of bacterial pathogens.Methods:  Preparation  of  in  situ  surface-modified  copper  doped  ZnOnanoparticles (Cu:ZnO NPs was done according to standard procedure.Assay for antimicrobial activity of Cu:ZnO NPs against bacterial pathogens was carried out based on disc diffusion method. Determination of shelf life, thermal and pH stability of antibacterial activity of Cu:ZnO NPs was done and residual activity was determined against the target cultures.Results: FTIR spectra indicate that the nanomaterials synthesized have higher peak intensity compared with reagent grade ZnO. According to the SEM image the nanoparticles synthesized have different size and heterogeneous morphology.  400  ppm  of  Cu:ZnO  NPs  gave  zones  of  inhibition  with diameters of 9.0 – 16 mm against the target cultures. Amongst the target cultures, Escherchia coli was the most sensitive to the Cu:ZnO NPs inhibition zone diameter 16 mm; whereas, 9 mm wide inhibition zone was obtained against  Staphylococcus  aureus. The  Cu:ZnO  NPs  was  fairly stable  for a period of 60 days at room temperature (RT showing lost of only 20% and30%     antibacterial  activity  as  tested  against  E.  coli  and  S.  aureus, respectively. The Cu:ZnO NPs was quite stable at this pH and temperaturerange tested against both E. coli and S. aureus.Conclusion: Surface-modified copper doped Cu:ZnO NPs have significant potential for their usefulness as antibacterial agents.

  4. Coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites for the detection of BCR/ABL fusion gene

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Xueping [Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 (China); Wang, Li [Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 (China); Department of Medical Laboratory, Chongqing Emergency Medical Center (Chongqing The Fourth Hospital), Chongqing, 400016 (China); Sheng, Shangchun [The No.2 Peoples' Hospital of Yibin, Sichuan, 644000 (China); Wang, Teng; Yang, Juan [Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 (China); Xie, Guoming, E-mail: guomingxie@cqmu.edu.cn [Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 (China); Feng, Wenli, E-mail: fengwlcqmu@sina.com [Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 (China)

    2015-08-19

    This article described a novel method by coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites (GS/PANI/AuNPs) for highly sensitive and specific detection of BCR/ABL fusion gene (bcr/abl) in chronic myeloid leukemia (CML). DNA circuit known as catalyzed hairpin assembly (CHA) is enzyme-free and can be simply operated to achieve exponential amplification, which has been widely employed in biosensing. However, application of CHA has been hindered by the need of specially redesigned sequences for each single-stranded DNA input. Herein, a transducer hairpin (HP) was designed to obtain a universal DNA circuit with favorable signal-to-background ratio. To further improve signal amplification, GS/PANI/AuNPs with excellent conductivity and enlarged effective area were introduced into this DNA circuit. Consequently, by combining the advantages of CHA and GS/PANI/AuNPs, bcr/abl could be detected in a linear range from 10 pM to 20 nM with a detection limit of 1.05 pM. Moreover, this protocol showed excellent specificity, good stability and was successfully applied for the detection of real sample, which demonstrated its great potential in clinical application. - Highlights: • A transducer hairpin was designed to improve the versatility of DNA circuit. • GS/PANI/AuNPs were introduced to the DNA circuit for further signal amplification. • The established biosensor displayed high sensitivity and good specificity.

  5. Does Physical Production of Nanoparticles Reduce Their Ecotoxicity? A Case of Lower Toxicity of AgNPs Produced by Laser Ablation to Zebrafish (Danio rerio

    Directory of Open Access Journals (Sweden)

    Seyed Ali Johari

    2014-09-01

    Full Text Available Use of nano-materials has increased in various aspects of human life. However, possible outbreak of nano-materials toxicity in humans and other organisms is one of the future challenges. Different chemical precursors which are used in chemical approaches for production of nano-materials may have secondary and sometimes toxic effects in living organisms. These secondary effects may be reduced in physical approaches due to not use of chemicals. To test this hypothesis, acute toxic effects of two types of silver nanoparticles (AgNPs which were produced by physical (top-down and chemical (bottom-up methods on survival rate of Zebrafish (Danio rerio were compared. According to the results, AgNPs produced by physical method were 38 times less toxic than ones generated by chemical method and therefore, the hypothesis was approved. The estimated 96 hr LC50 values of AgNPs produced by physical and chemical methods for zebra fish were 0.540 ± 0.032 and 0.014 ± 0.001 mg/L, respectively. According to these values and regarding the rules of European Union, both types of AgNPs are considered as highly toxic chemicals to aquatic organisms. Generally, AgNPs seems to have toxic effects on aquatic organisms regardless of the method used for their production, and so, their accidental or intentional entrance into the aquatic ecosystems should be inhibited.

  6. Enhancement of TiO2 NPs Activity by Fe3O4 Nano-Seeds for Removal of Organic Pollutants in Water

    Directory of Open Access Journals (Sweden)

    Silvia Villa

    2016-09-01

    Full Text Available The enhancement of the photocatalytic activity of TiO2 nanoparticles (NPs, synthesized in the presence of a very small amount of magnetite (Fe3O4 nanoparticles, is here presented and discussed. From X-ray diffraction (XRD and differential scanning calorimetry (DSC analyses, the crystallinity of TiO2 nanoparticles (NPs seems to be affected by Fe3O4, acting as nano-seeds to improve the tetragonal TiO2 anatase structure with respect to the amorphous one. Photocatalytic activity data, i.e., the degradation of methylene blue and the Ofloxacin fluoroquinolone emerging pollutant, give evidence that the increased crystalline structure of the NPs, even if correlated to a reduced surface to mass ratio (with respect to commercial TiO2 NPs, enhances the performance of this type of catalyst. The achievement of a relatively well-defined crystal structure at low temperatures (Tmax = 150 °C, preventing the sintering of the TiO2 NPs and, thus, preserving the high density of active sites, seems to be the keystone to understand the obtained results.

  7. Photoconversion of 4-nitrophenol in the presence of hydrazine with AgNPs-TiO{sub 2} nanoparticles prepared by the sol–gel method

    Energy Technology Data Exchange (ETDEWEB)

    Hernández-Gordillo, Agileo, E-mail: agileo12@hotmail.com [División de Materiales Avanzados, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055 Col. Lomas 4a. sección, C.P. 78216 San Luis Potosí, SLP, México (Mexico); CIIEMAD, Instituto Politécnico Nacional, Calle 30 de Junio de 1520 s/n, Barrio la Laguna Ticomán, C.P. 07340, Del. Gustavo A. Madero, DF, México (Mexico); Arroyo, Missael [División de Materiales Avanzados, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055 Col. Lomas 4a. sección, C.P. 78216 San Luis Potosí, SLP, México (Mexico); Zanella, R. [Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, A.P. 70-186, Delegación Coyoacán, C.P. 04510 México, DF, México (Mexico); and others

    2014-03-01

    Graphical abstract: - Highlights: • AgNPs are well dispersed on TiO{sub 2} semiconductor surface. • TiO{sub 2} with 1.5 wt% of Ag{sup 0} is the most photoactive for the 4-nitrophenol conversion. • High conversion of 4-nitrofenol to 4-aminophenol was achieved with hydrazine. • Enhanced photoactivity of Ag-TiO{sub 2} is associated with the ability to oxidize hydrazine. - Abstract: The photocatalytic properties of functionalized TiO{sub 2} with silver nanoparticles (AgNPs) for the conversion of 4-nitrophenol to 4-aminophenol in the presence of hydrazine were investigated. The TiO{sub 2} semiconductor synthesized by the sol–gel method was functionalized with AgNPs at different loadings, and their structural and optical properties were characterized by several techniques. The functionalized TiO{sub 2} with 1.5 wt% AgNPs presented the highest photocatalytic activity for the conversion of 4-nitrophenol with appropriate hydrazine concentrations (0.5 M). The photoefficiency enhancement under UV light irradiation was attributed to the electron transfer from the TiO{sub 2} semiconductor surface to the adsorbed acceptor reactant (4-nitrophenol) through the deposited AgNPs.

  8. Metabolic responses of the growing Daphnia similis to chronic AgNPs exposure as revealed by GC-Q-TOF/MS and LC-Q-TOF/MS.

    Science.gov (United States)

    Zhang, Bo; Zhang, Hui; Du, Chunlei; Ng, Qin Xiang; Hu, Chaoyang; He, Yiliang; Ong, Choon Nam

    2017-05-01

    Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials. Their fast-growing utilization has increased the occurrence of AgNPs in the environment, posing potential health and ecological risks. In this study, we conducted chronic toxicity tests and investigated the metabolic changes of the growing Daphna similis with exposure to 0, 0.02, and 1 ppb AgNPs, using non-targeted mass spectrometry-based metabolomics. To the best of our knowledge, this study is the first to report the baseline metabolite change of a common aquatic organism Daphnia crustacean through its life-cycle. The results show a dynamic kinetic pattern of the growing Daphnia's metabolome underwent a cycle from day 0 to day 21, with the level of metabolites gradually increasing from day 0 to day 13, before falling back to the baseline level of day 0 on day 21. As for the samples exposed to environmental concentrations of AgNPs, although without morphological or structural changes, numerous metabolite changes occurred abruptly during the first 10 days, and these changes reached steady state by day 13. The significant changes in certain metabolites, such as amino acids (serine, threonine and tyrosine), sugars (d-allose) and fatty acids (arachidonic acid) revealed new insights into how these metabolites in Daphnia respond to chronic AgNPs stress. These findings highlight the capability of metabolomics to discover early metabolic responses to environmental silver nanoparticles. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Core-Shell γ-Fe2O3/SiO2/PCA/Ag-NPs Hybrid Nanomaterials as a New Candidate for Future Cancer Therapy

    Science.gov (United States)

    Soleyman, R.; Pourjavadi, A.; Masoud, N.; Varamesh, A.

    2014-04-01

    In the current study, γ-Fe2O3/SiO2/PCA/Ag-NPs hybrid nanomaterials were successfully synthesized and characterized. At first, prepared γ-Fe2O3 core nanoparticles were modified by SiO2 layer. Then they were covered by poly citric acid (PCA) via melting esterification method as well. PCA shell acts as an effective linker, and provides vacancies for conveying drugs. Moreover, this shell as an effective capping agent directs synthesis of silver nanoparticles (Ag-NPs) via in situ photo-reduction of silver ions by sunlight-UV irradiation. This system has several benefits as a suitable cancer therapy nanomaterial. Magnetic nanoparticles (MNPs) can guide Ag-NPs and drugs to cancer cells and then Ag-NPs can affect those cells via Ag-NPs anti-angiogenesis effect. Size and structure of the prepared magnetic hybrid nanomaterials were characterized using FTIR and UV-Vis spectra, AFM and TEM pictures and XRD data.

  10. Improved nonlinear optical and optical limiting properties in non-covalent functionalized reduced graphene oxide/silver nanoparticle (NF-RGO/Ag-NPs) hybrid

    Science.gov (United States)

    Sakho, El hadji Mamour; Oluwafemi, Oluwatobi S.; Sreekanth, P.; Philip, Reji; Thomas, Sabu; Kalarikkal, Nandakumar

    2016-08-01

    Nonlinear optical (NLO) response under near infrared (800 nm) and visible (532 nm) laser excitations, of 100 fs (fs) and 5 ns (ns) pulse durations respectively, of reduced graphene oxide (RGO), non-covalent functionalized reduced graphene oxide (NF-RGO) and NF-RGO decorated with various concentration of silver nanoparticles (NF-RGO/Ag-NPs) have been investigated using the Open-aperture Z-Scan technique. For both femtosecond and nanosecond laser excitations, the studied graphene-based materials exhibit good nonlinear optical power limiting properties (OL), with NF-RGO/Ag-NPs sample prepared with 0.1 M AgNO3 showing the best nonlinear optical properties. For the ns regime, the optical limiting threshold decreased from 8.3 J/cm2 in NF-RGO to 4.3 J/cm2 in NF-RGO/Ag-NPs, while at fs regime, the nonlinear absorption coefficient (β) was found to increase with decrease in concentration of Ag-NPs in the hybrid. Two-photon absorption (2 PA) in combination with saturable absorption (SA) in femtosecond regime, and reverse saturable absorption (RSA) along with saturable absorption (SA) in the nanosecond regime, are responsible for the observed nonlinear optical absorption (NLA) behavior in these materials. These findings show that the as-synthesized NF-RGO/Ag-NPs hybrid is a relatively better material for nonlinear optical limiting applications.

  11. Effects of biochar and lime additives on non-point load of heavy metals in paddy soil%生物炭与石灰添加对稻田土壤重金属面源负荷影响

    Institute of Scientific and Technical Information of China (English)

    刘孝利; 曾昭霞; 陈求稳; 邹慧玲

    2014-01-01

    Owing to the strong ability to immobilize some toxic heavy metals immobilization in contaminated soils,biochar and lime received increasing interests from the community of soil remediation and crops safe⁃ty in recent years. This study conducted a two-year in situ field experiment in a paddy soil which had been contaminated by complex heavy metals,in Hunan province,China. The objective is to investigate the effects of different treatments of biochars and lime additives on the solution and non-pointe load of Cd, Pb,Zn. The additive rate was 20 t/hm2 for biochars and 750 kg/hm2 for lime. The results show that rice bio⁃char additive evidently reduced soluble Cd, Pb, Zn concentrations in waterlogged paddy by 56 %~80 %, 60 %~75 %, 63 %~90 % respectively, and bean biochar additive reduced soluble Cd, Pb, Zn concentra⁃tions by 61%~83%,51%~76% and 55%~80% respectively,comparing to the control treatment. No ob⁃vious changes of soluble Cd, Pb, Zn, As concentrations was observed in the treatment of lime application compared to the control treatment. However,both rice and bean biochar additives significantly increased sol⁃uble metalloid As concentrations in waterlogged paddy,compared to the lime additive and the control treat⁃ments. In conclusion, both rice and bean biochar applications could significantly decrease the non-point load of Cd, Pb and Zn during rainfall events, while increase the non-point load of metalloid As, leading to higher risk of As contamination of the receiving water. No obvious seasonality of non-point load of Cd, Pb,Zn and As was observed in this study,which may be due to the large variation of precipitation.%生物炭、石灰以其良好的有毒金属固化与生态环境效应成为近年来土壤污染修复及作物安全生产研究的热点。选取典型重金属复合污染水稻田开展原位实验,分析不同原料生物炭、石灰添加处理对稻田淹水溶解态 Cd、Pb、Zn、As析出特征及其自然降雨事件面

  12. Physical and thermo-mechanical properties of bionano reinforced poly(butylene adipate-co-terephthalate, hemp/CNF/Ag-NPs composites

    Directory of Open Access Journals (Sweden)

    Harikrishnan Pulikkalparambil

    2017-07-01

    Full Text Available A facile approach to prepare bionanocomposites of poly(butylene adipate-co-terephthalate (PBAT is reported in this paper. The effect of different wt% of hemp/Sihemp, carbon nanofiber (CNF and silver nanoparticle (Ag-NPs on the density, water absorption, melting and crystallization behavior, thermal stability, mechanical properties and morphology was investigated. The density of the composites was reduced except for Ag-NPs reinforced nanocomposites while diffusion coefficient and maximum water absorption were decreased for Sihemp reinforced composites making it a suitable material to replace conventional polymers. Significant improvement in tensile strength (TS and tensile modulus (TM was observed for the PBAT/Sihemp composites. For CNF and Ag-NPs reinforced nanocomposites, mechanical properties were retained at lower filler concentration. But as the concentration increased, there was a tendency for the nanofillers to agglomerate, which resulted in a reduction in mechanical properties.

  13. One-step synthesis of graphene-AuNPs by HMTA and the electrocatalytical application for O2 and H2O2.

    Science.gov (United States)

    Hu, Jianguo; Li, Fenghua; Wang, Kaikai; Han, Dongxue; Zhang, Qixian; Yuan, Junhua; Niu, Li

    2012-05-15

    A green, one-step method for synthesis of graphene-Au nanoparticles (graphene-AuNPs) was introduced in this article, using an environmentally benign hexamethylenetetramine (HMTA) as reducing and stabilizing agent. HMTA slowly was hydrolyzed to generate aldehyde ammonia to reduce graphene oxides (GO) and hydrogen tetrachloroaurate (Au precursor). The structure and composition of the graphene-AuNPs nanocomposites were studied by means of ultraviolet visible (UV) absorption spectra, X-ray photoelectron spectroscopy (XPS) and Transmission electron microscopy (TEM). The AuNPs are well-dispersed on graphene nanosheets in narrow size range. The nanocomposites have excellent electrocatalytical properties for catalytic reduction of O(2) and H(2)O(2).

  14. Anthelmintic Effect of Biocompatible Zinc Oxide Nanoparticles (ZnO NPs on Gigantocotyle explanatum, a Neglected Parasite of Indian Water Buffalo.

    Directory of Open Access Journals (Sweden)

    Yasir Akhtar Khan

    Full Text Available Helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. With the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. In the present study, biocompatible zinc oxide nanoparticles (ZnO NPs were used to see their in vitro effect on the biliary amphistomes, Gigantocotyle explanatum, infecting Bubalus bubalis because these nanoparticles are involved in generation of free radicals that induce oxidative stress, resulting in disruption of cellular machinery. The ZnO NPs were synthesized by using egg albumin as a biotemplate and subsequently characterized by Scanning Electron Microscopy (SEM, Transmission Electron Microscopy (TEM, X-ray Diffraction and Spectrophotometrical, which showed that ZnO NPs were highly purified wurtzite type polycrystals, with a mean size of 16.7 nm. When the parasites were treated with lower concentrations (0.004% and 0.008% of the ZnO NPs, the worms mounted a protective response by stimulating the antioxidant system but the treatment of G. explanatum with 0.012% ZnO NPs produced significant inhibition of the antioxidant enzymes like superoxide dismutase (SOD (p< 0.05 and glutathione S- transferase (GST (p<0.01, while the level of malondialdehyde (MDA, a lipid peroxidation marker, was significantly (p< 0.01 elevated. SEM and histopathology revealed pronounced tegumental damage showing the disruption of surface papillae and the annulations, particularly in the posterior region near acetabulum. The under expression of a number of polypeptides, loss of worm motility in a time dependent manner, further reflect strong anthelmintic potential of ZnO NPs. It can be concluded that the anthelmintic effect might be due to the production of reactive oxygen species that target a variety of macromolecules such as nucleic acid, protein and lipids which are involved in different cellular processes.

  15. Comparative phytotoxicity of ZnO NPs, bulk ZnO, and ionic zinc onto the alfalfa plants symbiotically associated with Sinorhizobium meliloti in soil.

    Science.gov (United States)

    Bandyopadhyay, Susmita; Plascencia-Villa, Germán; Mukherjee, Arnab; Rico, Cyren M; José-Yacamán, Miguel; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge L

    2015-05-15

    ZnO nanoparticles (NPs) are reported as potentially phytotoxic in hydroponic and soil media. However, studies on ZnO NPs toxicity in a plant inoculated with bacterium in soil are limited. In this study, ZnO NPs, bulk ZnO, and ZnCl₂ were exposed to the symbiotic alfalfa (Medicago sativa L.)-Sinorhizobium meliloti association at concentrations ranging from 0 to 750 mg/kg soil. Plant growth, Zn bioaccumulation, dry biomass, leaf area, total protein, and catalase (CAT) activity were measured in 30 day-old plants. Results showed 50% germination reduction by bulk ZnO at 500 and 750 mg/kg and all ZnCl₂ concentrations. ZnO NPs and ionic Zn reduced root and shoot biomass by 80% and 25%, respectively. Conversely, bulk ZnO at 750 mg/kg increased shoot and root biomass by 225% and 10%, respectively, compared to control. At 500 and 750 mg/kg, ZnCl₂ reduced CAT activity in stems and leaves. Total leaf protein significantly decreased as external ZnCl₂ concentration increased. STEM-EDX imaging revealed the presence of ZnO particles in the root, stem, leaf, and nodule tissues. ZnO NPs showed less toxicity compared to ZnCl₂ and bulk ZnO found to be growth enhancing on measured traits. These findings are significant to reveal the toxicity effects of different Zn species (NPs, bulk, and ionic Zn) into environmentally important plant-bacterial system in soil.

  16. Anisotropic In Situ-Coated AuNPs on Screen-Printed Carbon Surface for Enhanced Prostate-Specific Antigen Impedimetric Aptasensor

    Science.gov (United States)

    Do, Tram T. N.; Van Phi, Toan; Nguy, Tin Phan; Wagner, Patrick; Eersels, Kasper; Vestergaard, Mun'delanji C.; Truong, Lien T. N.

    2016-12-01

    An impedimetric aptasensor has been used to study the effect of charge transfer on the binding of prostate-specific antigen (PSA) to its aptamer. Full understanding of this mechanism will be beneficial to further improve its sensitivity for PSA detection in human semen at physiologically relevant concentrations. Bare gold electrodes (SPAuEs) and gold nanoparticles (AuNPs)-coated screen-printed carbon ink electrodes (AuNPs/SPCEs) were coated with aptamer solution at various concentrations and the sensor response to increasing PSA concentration in buffer solution examined. AuNPs were deposited onto carbon electrodes in 10 cycles. AuNPs/SPCEs were then coated with a self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid prior to aptamer immobilization at dose of 5 μg mL-1. The results indicate that anisotropic AuNPs/SPCEs outperform bare gold electrodes in terms of decreased amount of aptamer bunches as well as the number of intermediate PSA-aptamer complexes formed on the electrode surface. The key finding is that the fabricated aptasensor is sensitive enough [limit of detection (LoD) 1.95 ng mL-1] for early diagnosis of prostate cancer and displays linear response in the physiologically relevant concentration range (0 ng mL-1 to 10 ng mL-1), as shown by the calibration curve of the relative change in electron transfer resistance (ΔR CT) versus PSA concentration when aptamer/SAM/AuNPs/SPCEs were exposed to buffer containing PSA at different concentrations.

  17. Identifying English BaseNPs Through a Combination of Maximum Entropy Approach and Brill Approach%最大熵和Brill方法结合识别英语BaseNPs

    Institute of Scientific and Technical Information of China (English)

    吕琳; 刘玉树

    2006-01-01

    为了进一步提高基本名词短语(BaseNPs)的识别精度,针对最大熵方法和Brill方法各自的特点,提出基于两者相结合的英语基本名词短语识别算法.该算法是在高准确率词性标注的基础上实现的.在训练和测试两个阶段中,均先采用最大熵方法识别基本名词短语,然后将已具有很高精度的识别结果作为初始标注结果运用于Brill方法中.实验结果表明,此联合算法达到了94%的准确率和召回率,充分融合了最大熵方法和Brill方法的优点,可与基于相同训练和测试语料的目前最理想的英语基本名词短语识别结果相比.

  18. NPS Defense Affordability Panel

    Science.gov (United States)

    2012-05-01

    MEADS CAP AMRAAM (AIM-120) MQ-9 UAS REAPER AMF JTRS Chem Demil -ACWA TRIDENT II MISSILE USMC H-1 UPGRADES LHA 6 JLENS 100 50 0 50 100 150 200 250 300...DDG 1000, E-2D AHE, EFV(2)**, RMS, VH-71* – Air Force: AEHF, C-5 RERP, C-130 AMP, EELV(2), Global Hawk, JASSM, JPATS, WGS – DoD: Chem Demil -ACWA(2...Javelin, JLENS – Navy: H-1 Upgrades – Air Force: AEHF, C-27J, NPOESS, C-130 AMP – DoD: Chem Demil -ACWA, JTRS GMR * This program reported

  19. Solution-Processed rGO/AgNPs/rGO Sandwich Structure as a Hole Extraction Layer for Polymer Solar Cells

    Directory of Open Access Journals (Sweden)

    Quang Trung Tran

    2015-01-01

    Full Text Available We found that inserting silver nanoparticles (AgNPs between two layers of reduced grapheme oxide (rGO has an effect on tailoring the work function of rGO. The utilization of rGO/AgNPs/rGO sandwich structure as the hole extraction layer in polymer solar cells is demonstrated. Solution-processable fabrication of this sandwich structure at the ITO/active layer interface facilitates the extraction of hole from active layer into ITO anode because of lowering the barrier level alignment at the interface. It results in an improvement of the short circuit current density and the overall photovoltaic performance.

  20. United Nations deliberations of the use of nuclear power sources in space: 1978-1987

    Science.gov (United States)

    Bennett, Gary L.; Sholtis, Joseph A., Jr.; Rashkow, Bruce C.

    1988-01-01

    The United Nations (U.N.) is continuing its deliberations on the use of nuclear power sources (NPS) in space. Although no complete set of legal principles has yet been agreed upon, certain scientific and technical criteria for the safe design and use of NPS have been accepted. In this respect, it should be noted that in its 1981 report, the Working Group on the Use of Power Sources in Outer Space concluded that power sources can be used safely in outer space, provided that all necessary safety requirements are met. This is also a succinct statement of the U.S. position.

  1. Proteomic response of mussels Mytilus galloprovincialis exposed to CuO NPs and Cu{sup 2+}: An exploratory biomarker discovery

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Tânia, E-mail: tania.gomes@niva.no; Chora, Suze; Pereira, Catarina G.; Cardoso, Cátia; Bebianno, Maria João

    2014-10-15

    Highlights: • Different protein expression patterns, tissue and Cu form dependent. • Different cellular mechanisms involved in CuO NPs and Cu{sup 2+} toxicity. • CuO NPs toxicity mediated by cell signalling cascades that result in apoptosis. • Caspase 3/7–1, catL, Zn-finger, precol-D as new molecular targets for both Cu forms in mussels. - Abstract: CuO NPs are one of the most used metal nanomaterials nowadays with several industrial and other commercial applications. Nevertheless, less is known about the mechanisms by which these NPs inflict toxicity in mussels and to what extent it differs from Cu{sup 2+}. The aim of this study was to investigate changes in protein expression profiles in mussels Mytilus galloprovincialis exposed for 15 days to CuO NPs and Cu{sup 2+} (10 μg L{sup −1}) using a proteomic approach. Results demonstrate that CuO NPs and Cu{sup 2+} induced major changes in protein expression in mussels’ showing several tissue and metal-dependent responses. CuO NPs showed a higher tendency to up-regulate proteins in the gills and down-regulate in the digestive gland, while Cu{sup 2+} showed the opposite tendency. Distinctive sets of differentially expressed proteins were found, either common or specific to each Cu form and tissue, reflecting different mechanisms involved in their toxicity. Fifteen of the differentially expressed proteins from both tissues were identified by MALDI-TOF-TOF. Identified proteins indicate common response mechanisms induced by CuO NPs and Cu{sup 2+}, namely in cytoskeleton and cell structure (actin, α-tubulin, paramyosin), stress response (heat shock cognate 71, putative C1q domain containing protein), transcription regulation (zinc-finger BED domain-containing protein 1, nuclear receptor subfamily 1G) and energy metabolism (ATP synthase F0 subunit 6). CuO NPs alone also had a marked effect on other biological processes, namely oxidative stress (GST), proteolysis (cathepsin L) and apoptosis (caspase 3/7-1). On

  2. Determination of Gallic Acid by Flow Injection Analysis Based on LuminoI-AgNO3-Ag NPs Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    李世凤; 孙惠敏; 王东; 钱莉; 朱燕; 陶善军

    2012-01-01

    A novel flow injection procedure has been developed for the determination of gallic acid based on the en- hancement function for luminol-AgNO3-Ag NPs chemiluminescence (CL) system by gallic acid. The enhancement mechanism was proposed for the reinforcing effect of the gallic acid on the CL system. The UV-vis absorption spectrum and CL emission spectrum were applied to confirm the mechanism. The method is simple, rapid and sen- sitive with a detection limit of 5 × 10^-10 g.mL-l and a linear range of 8.0 × 10^-10- 1.0 × 10-7 g.mL-1. The relative standard deviation (RSD) is 1.3% for eleven measurements of 5 × 10^-8 g-mL-1 gallic acid. The method has been successfully applied to the determination of gallic acid in Chinese proprietary medicine--Jianmin Yanhou tablets and synthesized samples.

  3. PDMS-based Optical Leaky Waveguide Coated with Self-assemble Au-NPs for Bio-analytical Detections

    Directory of Open Access Journals (Sweden)

    Yi-Chieh Chen

    2012-03-01

    Full Text Available This paper presents a novel method for fabricating PDMS-based optical leaky waveguides coated with self-assembled gold nano-particles (Au-NP for bio-analytical detection utilizing the localized surface plasmon resonance (LSPR effect. In the presented method, a PDMS optical waveguide is first cast in Teflon tubing to form a cylindrical leaky waveguide structure. The de-molded PDMS optical waveguide is then modified with PDDA molecules and coated with a layer of 13 nm Au-NPs for inducing the LSPR effect. The fabricated LSPR sensor is finally connected to multi-mode optic fibers for guiding the detection light. The measured sensitivity of the PDMS waveguide based LSPR sensor for detecting diluted glycerol solutions was 7.25 AU/RIU and 325.97 nm/RIU. Experimental results of a label-free detection of DNA hybridization show that the presented PDMS waveguide based LSPR sensor has a good linear response and has a detection limit of about 10pM, confirming the detection performance of the developed PDMS waveguide-based LSPR sensor.

  4. Role of Carboxylate ligands in the Synthesis of AuNPs: Size Control, Molecular Interaction and Catalytic Activity

    KAUST Repository

    Aljohani, Hind Abdullah

    2016-05-22

    Nanoparticles (NPs) are the basis of nanotechnology and finding numerous applications in various fields such as health, electronics, environment, personal care products, transportation, and catalysis. To fulfill these functions, the nanoparticles must be synthesized, passivated to control their chemical reactivity, stabilized against aggregation and functionalized to achieve specific performances. The chemistry of metal nanoparticles especially that of noble metals (Gold, Platinum…) is a growing field. The nanoparticles have indeed different properties from those of the corresponding bulk material. These properties are largely influenced by several parameters; the most important are the size, shape, and the local environment of the nanoparticles. One of the most common synthetic methods for the preparation of gold nanoparticles (AuNPs) is based on stabilization by citrate. Since it was reported first by Turkevich et al. in 1951, this synthetic scheme has been widely used, studied and a substantial amount of important information regarding this system has been reported in the literature. The most popular method developed by Frens for controlling the size of the noble gold nanoparticles based on citrate was achieved by varying the concentration of sodium citrate. Despite a large number of investigations focused on utilizing Cit-AuNPs, the structural details of citrate anions adsorbed on the AuNP surface are still unknown. It is known only that citrate anions “coordinate” to the metal surface by inner sphere complexation of the carboxylate groups and there are trace amounts of AuCl4−, Cl−, and OH− on the metal surface. Moreover, it is generally accepted that the ligand shell morphology of Au nanoparticles can be partly responsible for important properties such as oxidation of carbon monoxide. The use of Au-NPs in heterogeneous catalysis started mostly with Haruta who discovered the effect of particle size on the activity for carbon monoxide oxidation at

  5. Physicochemical and biological properties of hydrogel/gelatin/hydroxyapatite PAA/G/HAp/AgNPs composites modified with silver nanoparticles.

    Science.gov (United States)

    Sobczak-Kupiec, Agnieszka; Malina, Dagmara; Piatkowski, Marek; Krupa-Zuczek, Kinga; Wzorek, Zbigniew; Tyliszczak, Bozena

    2012-12-01

    Composites comprising biodegradable polymer matrix, bioactive ceramic fillers and metallic nanoparticles can be applied in the substitution of bone tissue and many others medical and dental applications. Recently, fully resorbable composite materials applicable as bone substitutes are the subject of intensive studies in surgical reconstruction and bone tissue engineering. Biological composites, such as bone and teeth, contain hydroxyapatite (HAp), a mineral component with composition Ca10(PO4)6(OH)2. Silver nanoparticles or silver ions have long been known to have strong inhibitory and bactericidal effects as well as a broad spectrum of antimicrobial activities. In this study we applied natural origin hydroxyapatite obtained from pork bone sludge. As polymer matrix gelatin and poly(acrylic acid) were used. Composite materials were obtained with the use of microwave irradiation. The influence of metallic nanoparticles presence on the degradation process of composite materials was investigated by pH and conductivity analyses of water filtrates. In vitro tests in simulated body fluid (SBF) and artificial saliva confirmed that it is possible to produce hydroxyapatite/polymer composites doped with silver nanoparticles for medical applications. Tests proved that content of silver nanoparticles in composites had influence on degradation behaviour of HA/Polymer/AgNPs in artificial media such as simulated body fluid and saliva.

  6. Biocompatible synthesis of silver nanoparticles (AgNPs using marine Vibrio sp. and study their pharmacological applications

    Directory of Open Access Journals (Sweden)

    M. Surya

    2015-09-01

    Full Text Available This report presents a rapid and a green biogenic approach for the biosynthesis of silver nanoparticles using marine bacteria such as Vibrio sp. The bioactive compounds of Vibrio sp. induced the reduction of Ag+ ions from AgNO3, which resulted in the formation of bacterial mediated silver nanoparticles (AgNPs. The reduction of Ag+ ions to elemental silver was characterized by UV-vis spectroscopy, Fourier transform infra-red spectroscopy (FTIR, Scanning electron microscopy (SEM and Dynamic light scattering (DLS. The nanoparticles show maximum absorbance at 421 nm on ultraviolet-visible spectra. The presence of biomolecules were identified by Fourier transform–infrared spectroscopy. The bioactive compounds and nanoparticles were found to direct different shape and sized and their size/ shape were varied. The silver nanoparticle synthesized were spherical, in the range of 40-80 nm in size. In antimicrobial screening, all the pathogens were fairly affected and nil effect was not observed in the test samples, In bacteria, the test sample was most effective against Enterococcus faecalis B2 and Salmonella typhimurium B5. In fungi, which was effective against Candida albicans F1, Microsporum canis F3 and Trichophyton rubrum F4.

  7. The Electrochemical Behavior of Au/AuNPs/PNA/ZnSe-QD/ACA Electrode Towards CySH Oxidation

    Institute of Scientific and Technical Information of China (English)

    Azadeh Azadbakht; Amir Reza Abbasi; Zohreh Derikvand; Ziba Karimi

    2015-01-01

    This work describes the electrochemical behavior of azodicarboxamide (ACA) film immobilized on the surface of penicillamine (PNA)/ZnSe-quantum dot (ZnSe-QD) gold nanoparticle (AuNPs) Au electrode. Electrocatalytic activity of modified electrode toward the oxidation of cysteine (CySH) was investigated. The surface structure and composition of the sensor were characterized by scanning electron microscopy (SEM). Oxidation of CySH on the surface of modified electrode was investigated with cyclic voltammetry, electrochemical impedance spectroscopy (EIS), hydrodynamic voltammetry and chronoamperometry methods. The results show that the PNA/ZnSe-QD/ACA film displays excellent electrochemical catalytic activities towards CySH oxidation. The modified electrode shows repro-ducible behavior and high level of stability during the electrochemical experiments. Also it has short response time, low detection limit, high sensitivity and low operation potential, which can be used as an amperometric sensor for moni-toring of CySH. The proposed modified electrode was successfully used for determination of CySH in real sample such as human serum.

  8. Spatial and Seasonal Variations of Heavy Metal Loads in Uyo Urban Drainage Stream under PS and NPS Pollution

    Directory of Open Access Journals (Sweden)

    O. E. Essien

    2012-12-01

    Full Text Available The concentration of heavy metals (HM in the 4km urban drainage stream in Uyo was studied using grab and composite sampling of water from six stations on the stream, and analyzed by a multipurpose atomic absorption spectrophotometer. The data were statistically analyzed using SPSS version 17 software arid correlated between stations and among samples. HM contamination was evaluated with the coefficient and rate of dispersion between stations and the Normalized Scatter Coefficient (NSC. The seasonal distribution of metal pollution varied individually amongst metals at stations. The Fe and Pb concentrations exceeded the safe drinking water standard, rendering the water quality not acceptable for drinking; however, the quality was within the safe limit for crop production along the river bank. The relative.dominance of heavy metals followed a different sequence in upstream leachate effluent from all downstream stations. The NSC in dry-wet season was higher than in wet season, and was in the order: Fe>Cu>Zn>Pb, showing that large but variable concentration of Fe from PS and NPS contaminated the stream at faster rate in the wet season while Pb contaminated at nearly constant rate. However, in the dry season, Fe and Pb depleted at downstream at rates far higher than Zn as Cu was increasing. Fe and Pb could be good pollution monitor for total maximum daily load (TMDL pollution monitoring programme.

  9. Comparative effects on rat primary astrocytes and C6 rat glioma cells cultures after 24-h exposure to silver nanoparticles (AgNPs)

    Energy Technology Data Exchange (ETDEWEB)

    Salazar-García, Samuel; Silva-Ramírez, Ana Sonia; Ramirez-Lee, Manuel A.; Rosas-Hernandez, Hector [Universidad Autonoma de San Luis Potosi, Facultad de Ciencias Quimicas (Mexico); Rangel-López, Edgar [Instituto Nacional de Neurologia y Neurocirugia Manuel Velasco Suárez, Laboratorio de Aminoacidos Excitadores (Mexico); Castillo, Claudia G. [Facultad de Medicina, Universidad Autonoma de San Luis Potosi (Mexico); Santamaría, Abel [Instituto Nacional de Neurologia y Neurocirugia Manuel Velasco Suárez, Laboratorio de Aminoacidos Excitadores (Mexico); Martinez-Castañon, Gabriel A. [Universidad Autonoma de San Luis Potosi, Facultad de Estomatologia (Mexico); Gonzalez, Carmen, E-mail: cgonzalez.uaslp@gmail.com, E-mail: gonzalez.castillocarmen@fcq.uaslp.mx [Universidad Autonoma de San Luis Potosi, Facultad de Ciencias Quimicas (Mexico)

    2015-11-15

    The aim of this work was to compare the effects of 24-h exposure of rat primary astrocytes and C6 rat glioma cells to 7.8 nm AgNPs. Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor and current treatments lead to diverse side-effects; for this reason, it is imperative to investigate new approaches, including those alternatives provided by nanotechnology, like nanomaterials (NMs) such as silver nanoparticles. Herein, we found that C6 rat glioma cells, but no primary astrocytes, decreased cell viability after AgNPs treatment; however, both cell types diminished their proliferation. The decrease of glioma C6 cells proliferation was related with necrosis, while in primary astrocytes, the decreased proliferation was associated with the induction of apoptosis. The ionic control (AgNO{sub 3}) exerted a different profile than AgNPs; the bulk form did not modify the basal effect in each determination, whereas cisplatin, a well-known antitumoral drug used as a comparative control, promoted cytotoxicity in both cell types at specific concentrations. Our findings prompt the need to determine the fine molecular and cellular mechanisms involved in the differential biological responses to AgNPs in order to develop new tools or alternatives based on nanotechnology that may contribute to the understanding, impact and use of NMs in specific targets, like glioblastoma cells.

  10. High-throughput screening for new psychoactive substances (NPS) in whole blood by DLLME extraction and UHPLC-MS/MS analysis.

    Science.gov (United States)

    Odoardi, Sara; Fisichella, Marco; Romolo, Francesco Saverio; Strano-Rossi, Sabina

    2015-09-01

    The increasing number of new psychoactive substances (NPS) present in the illicit market render their identification in biological fluids/tissues of great concern for clinical and forensic toxicology. Analytical methods able to detect the huge number of substances that can be used are sought, considering also that many NPS are not detected by the standard immunoassays generally used for routine drug screening. The aim of this work was to develop a method for the screening of different classes of NPS (a total of 78 analytes including cathinones, synthetic cannabinoids, phenethylamines, piperazines, ketamine and analogues, benzofurans, tryptamines) from blood samples. The simultaneous extraction of analytes was performed by Dispersive Liquid/Liquid Microextraction DLLME, a very rapid, cheap and efficient extraction technique that employs microliters amounts of organic solvents. Analyses were performed by a target Ultrahigh Performance Liquid Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) method in multiple reaction monitoring (MRM). The method allowed the detection of the studied analytes with limits of detection (LODs) ranging from 0.2 to 2ng/mL. The proposed DLLME method can be used as an alternative to classical liquid/liquid or solid-phase extraction techniques due to its rapidity, necessity to use only microliters amounts of organic solvents, cheapness, and to its ability to extract simultaneously a huge number of analytes also from different chemical classes. The method was then applied to 60 authentic real samples from forensic cases, demonstrating its suitability for the screening of a wide number of NPS.

  11. Silica (NPs) supported Fe (III) as a reusable heterogeneous catalyst for the one-pot synthesis of 1, 4-dihydropyridines under mild conditions

    Indian Academy of Sciences (India)

    Javad Safaei-Ghomi; Abolfazl Ziarati; Safura Zahedi

    2012-07-01

    A cheap and recyclable silica (NPs) supported Fe (III) was prepared as heterogeneous catalyst for the synthesis of various substituted 1,4-dihydropyridines via condensation of aldehydes with ethyl acetoacetate and ammonium acetate in ethanol. The products were separated from the catalyst simply by filtration and the catalyst could be recycled and reused for several times without noticeable decrease in the catalytic activity.

  12. Bioinspired polydopamine as the scaffold for the active AuNPs anchoring and the chemical simultaneously reduced graphene oxide: characterization and the enhanced biosensing application.

    Science.gov (United States)

    Tian, Juan; Deng, Sheng-Yuan; Li, Da-Li; Shan, Dan; He, Wei; Zhang, Xue-Ji; Shi, You

    2013-11-15

    We report here an efficient approach to enhance the performance of biosensing platform based on graphene or graphene derivate. Initially, graphene oxides (GO) nanosheets were reduced and surface functionalized by one-step oxidative polymerization of dopamine in basic solution at environment friendly condition to obtain the polydopamine (Pdop) modified reduced graphene oxides (PDRGO). The bioinspired surface was further used as a support to anchor active gold nanoparticles (AuNPs). The morphology and structure of the as-prepared AuNPs/PDRGO nanocomposite were investigated by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FT-IR). Electrochemical studies demonstrate that the as-prepared AuNPs/PDRGO hybrid materials possess excellent electrochemical properties and electrocatalytic activity toward the oxidation of NADH at low potential (0.1 V vs. SCE) with the fast response (15s) and the broad linear range (5.0 × 10(-8)-4.2 × 10(-5)M). Thus, this AuNPs/PDRGO nanocomposite can be further used to fabricate a sensitive alcohol biosensor using alcohol dehydrogenase (ADH), by simply incorporating the specific enzyme within the composite matrix with the aid of chitosan (Chit).

  13. From concept(ion) to life after death/the grave: The 'natural' history and life cycle(s) of novel psychoactive substances (NPS).

    Science.gov (United States)

    Corkery, John Martin; Orsolini, Laura; Papanti, Duccio; Schifano, Fabrizio

    2017-05-01

    A range of information needs should be met in order to better understand and predict the longevity/existence of novel psychoactive substances (NPS). This conceptual paper argues that one way of assessing how long a molecule may be around is to document how the life cycles or natural histories of 'traditional' drugs and NPS evolve. The earliest indication of the possible appearance of a new substance might be evidenced on the DeepWeb. However, this means they are less visible, in line with the clandestine nature of drug use and supply. Therefore, monitoring discussion groups/fora needs the development of new methods compared to those used in the Surface Net. Issues needing consideration in establishing NPS life cycles are outlined here, together with the probable outcomes that could result. The approach advocated means that it should be easier to identify which NPS are likely to come up or are emerging in real time, and, therefore, pre-empt/prevent their supply. Copyright © 2017 John Wiley & Sons, Ltd.

  14. Development of sensitive amperometric hydrogen peroxide sensor using a CuNPs/MB/MWCNT-C{sub 60}-Cs-IL nanocomposite modified glassy carbon electrode

    Energy Technology Data Exchange (ETDEWEB)

    Roushani, Mahmoud, E-mail: mahmoudroushani@yahoo.com; Bakyas, Kobra; Zare Dizajdizi, Behruz

    2016-07-01

    A sensitive hydrogen peroxide (H{sub 2}O{sub 2}) sensor was constructed based on copper nanoparticles/methylene blue/multiwall carbon nanotubes–fullerene–chitosan–ionic liquid (CuNPs/MB/MWCNTs–C{sub 60}–Cs–IL) nanocomposites. The MB/MWCNTs–C{sub 60}–Cs–IL and CuNPs were modified glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of copper nitrate solution, respectively. The physical morphology and chemical composition of the surface of modified electrode was investigated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), respectively. The electrochemical properties of CuNPs/MB/MWCNTs–C{sub 60}–Cs–IL/GCE were investigated by cyclic voltammetry (CV) and amperometry techniques and the sensor exhibited remarkably strong electrocatalytic activities toward the reduction of hydrogen peroxide. The peak currents possess a linear relationship with the concentration of H{sub 2}O{sub 2} in the range of 0.2 μM to 2.0 mM, and the detection limit is 55.0 nM (S/N = 3). In addition, the modified electrode was used to determine H{sub 2}O{sub 2} concentration in human blood serum sample with satisfactory results. - Highlights: • CuNPs/MB/MWCNT-C{sub 60}-Cs-IL/GC electrode was constructed by layer-by-layer method. • The catalytic performance of the sensor was studied with the use of amperometric technique. • The constructed sensor showed enhanced electrocatalytic activity toward the reduction of H{sub 2}O{sub 2}. • The CuNPs/MB/MWCNT-C{sub 60}-Cs-IL/GC electrode demonstrated high stability for the detection of H{sub 2}O{sub 2}.

  15. Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn(2+) Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots.

    Science.gov (United States)

    Ahmed, Bilal; Dwivedi, Sourabh; Abdin, Malik Zainul; Azam, Ameer; Al-Shaeri, Majed; Khan, Mohammad Saghir; Saquib, Quaiser; Al-Khedhairy, Abdulaziz A; Musarrat, Javed

    2017-01-25

    Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn(2+)) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO2(-) at 1240 cm(-1) and stretching of C-O ribose at 1060 cm(-1), suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn(2+) in plants.

  16. Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots

    Science.gov (United States)

    Ahmed, Bilal; Dwivedi, Sourabh; Abdin, Malik Zainul; Azam, Ameer; Al-Shaeri, Majed; Khan, Mohammad Saghir; Saquib, Quaiser; Al-Khedhairy, Abdulaziz A.; Musarrat, Javed

    2017-01-01

    Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn2+) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 ± 8.7%) in % mitotic index and increase in chromosomal aberrations (18 ± 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-à-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in ΔΨm were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO2− at 1240 cm−1 and stretching of C-O ribose at 1060 cm−1, suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn2+ in plants. PMID:28120857

  17. Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode.

    Science.gov (United States)

    Kim, Gang-Il; Kim, Kyung-Woo; Oh, Min-Kyu; Sung, Yun-Mo

    2010-03-15

    A new electrochemical technique for the detection of vascular endothelial growth factors (VEGFs) as a cancer-related biomarker is presented in this paper. Gold nanoparticles (Au NPs) were self-assembled onto an indium tin oxide (ITO) electrode to prepare a modified sandwich type electrochemical immunoassay platform. VEGF antibodies were cleaved into two half-fragments by 2-mercaptoethylamine-HCl (2-MEA) and the fragments were immobilized onto the Au NP substrates by their thiol groups. Through this strategy, randomly oriented attachment of antibodies was prevented which frequently occurs in a general use of whole antibody and reduces the number of available sites for the attachment of target molecules. VEGF target molecules were applied to the immunoelectrodes and they combined with the antibody fragments covering the Au NP electrode, forming antigen-antibody complexes. Then, ferrocene-tagged antibodies, which release electrons under a proper applied potential, were added to the system and they combined with the VEGF molecules pre-attached to the antibody fragments. The redox current of ferrocene measured by the differential pulse voltammetry (DPV) increased almost linearly from 1.27 x 10(-4) to 4.17 x 10(-4)A according to the increase in the concentration of the VEGF target molecules from 100 to 600 pg/ml. The measured current values represent the concentration of the VEGF since they are proportional to the number of ferrocene molecules which is in turn proportional to the concentration of VEGF target molecules. Using this modified sandwich immunoassay with the Au NP/ITO electrode, VEGFs as low as 100 pg/ml were detected with high specificity.

  18. Are the TiO2 NPs a "Trojan horse" for personal care products (PCPs) in the clam Ruditapes philippinarum?

    Science.gov (United States)

    Sendra, M; Pintado-Herrera, M G; Aguirre-Martínez, G V; Moreno-Garrido, I; Martin-Díaz, L M; Lara-Martín, P A; J, Blasco

    2017-10-01

    In recent years, increasing quantities of personal care products (PCPs) are being released into the environment. However, data about bioaccumulation and toxicity are scarce; and extraction and analytical approaches are not well developed. In this work, the marine clam Ruditapes philippinarum, selected as model organism, has been employed to investigate bioaccumulation, antioxidant enzyme activities and DNA damage due to exposure to TiO2 nanoparticles and bulk TiO2 (inorganic compounds that are frequent components of PCPs, plastics, paints and coatings, foods and disinfectant water treatments). We have also studied the joint effect of both forms of inorganic TiO2 combined with four organic compounds (mixture exposures) commonly used in PCPs: an antimicrobial (triclosan), a fragrance (OTNE) and two UV filters (benzophenone-3 and octocrylene). Bioaccumulation of the inorganic compound, TiO2, was almost immediate and constant over exposure time. With respect to the organic compounds in mixtures, they were mediated by TiO2 and bioaccumulation is driven by reduced size of the particles. In fact, nanoparticles can be considered as a vector to organic compounds, such as triclosan and benzophenone-3. After a week of depuration, TiO2 NPs and TiO2 bulk in clams showed similar levels of concentration. Some organic compounds with bioactivity (Log Kow >3), like OTNE, showed low depuration after one week. The joint action of the organic compound mixture and either of the two forms of TiO2 provoked changes in enzyme activity responses. However, for the mixtures, DNA damage was found only after the depuration period. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Micro- and Nanoscale Energetic Materials as Effective Heat Energy Sources for Enhanced Gas Generators.

    Science.gov (United States)

    Kim, Sang Beom; Kim, Kyung Ju; Cho, Myung Hoon; Kim, Ji Hoon; Kim, Kyung Tae; Kim, Soo Hyung

    2016-04-13

    In this study, we systematically investigated the effect of micro- and nanoscale energetic materials in formulations of aluminum microparticles (Al MPs; heat source)/aluminum nanoparticles (Al NPs; heat source)/copper oxide nanoparticles (CuO NPs; oxidizer) on the combustion and gas-generating properties of sodium azide microparticles (NaN3 MPs; gas-generating agent) for potential applications in gas generators. The burn rate of the NaN3 MP/CuO NP composite powder was only ∼0.3 m/s. However, the addition of Al MPs and Al NPs to the NaN3 MP/CuO NP matrix caused the rates to reach ∼1.5 and ∼5.3 m/s, respectively. In addition, the N2 gas volume flow rate generated by the ignition of the NaN3 MP/CuO NP composite powder was only ∼0.6 L/s, which was significantly increased to ∼1.4 and ∼3.9 L/s by adding Al MPs and Al NPs, respectively, to the NaN3 MP/CuO NP composite powder. This suggested that the highly reactive Al MPs and NPs, with the assistance of CuO NPs, were effective heat-generating sources enabling the complete thermal decomposition of NaN3 MPs upon ignition. Al NPs were more effective than Al MPs in the gas generators because of the increased reactivity induced by the reduced particle size. Finally, we successfully demonstrated that a homemade airbag with a specific volume of ∼140 mL could be rapidly and fully inflated by the thermal activation of nanoscale energetic material-added gas-generating agents (i.e., NaN3 MP/Al NP/CuO NP composites) within the standard time of ∼50 ms for airbag inflation.

  20. Reducing Nonpoint Source Pollution Through Collaboration: Policies and Programs Across the U.S. States

    Science.gov (United States)

    Hardy, Scott D.; Koontz, Tomas M.

    2008-03-01

    Nonpoint source (NPS) pollution has emerged as the largest threat to water quality in the United States, influencing policy makers and resource managers to direct more attention toward NPS prevention and remediation. In response, the United States Environmental Protection Agency (USEPA) spent more than 204 million in fiscal year (FY) 2006 on the Clean Water Act’s Section 319 program to combat NPS pollution, much of it on the development and implementation of watershed-based plans. State governments have also increasingly allocated financial and technical resources to collaborative watershed efforts within their own borders to fight NPS pollution. With increased collaboration among the federal government, states, and citizens to combat NPS pollution, more information is needed to understand how public resources are being used, by whom, and for what, and what policy changes might improve effectiveness. Analysis from a 50-state study suggests that, in addition to the average 35% of all Section 319 funds per state that are passed on to collaborative watershed groups, 35 states have provided financial assistance beyond Section 319 funding to support collaborative watershed initiatives. State programs frequently provide technical assistance and training, in addition to financial resources, to encourage collaborative partnerships. Such assistance is typically granted in exchange for requirements to generate a watershed action plan and/or follow a mutually agreed upon work plan to address NPS pollution. Program managers indicated a need for greater fiscal resources and flexibility to achieve water quality goals.

  1. Investigating the effects of point source and nonpoint source pollution on the water quality of the East River (Dongjiang) in South China

    Science.gov (United States)

    Wu, Yiping; Chen, Ji

    2013-01-01

    Understanding the physical processes of point source (PS) and nonpoint source (NPS) pollution is critical to evaluate river water quality and identify major pollutant sources in a watershed. In this study, we used the physically-based hydrological/water quality model, Soil and Water Assessment Tool, to investigate the influence of PS and NPS pollution on the water quality of the East River (Dongjiang in Chinese) in southern China. Our results indicate that NPS pollution was the dominant contribution (>94%) to nutrient loads except for mineral phosphorus (50%). A comprehensive Water Quality Index (WQI) computed using eight key water quality variables demonstrates that water quality is better upstream than downstream despite the higher level of ammonium nitrogen found in upstream waters. Also, the temporal (seasonal) and spatial distributions of nutrient loads clearly indicate the critical time period (from late dry season to early wet season) and pollution source areas within the basin (middle and downstream agricultural lands), which resource managers can use to accomplish substantial reduction of NPS pollutant loadings. Overall, this study helps our understanding of the relationship between human activities and pollutant loads and further contributes to decision support for local watershed managers to protect water quality in this region. In particular, the methods presented such as integrating WQI with watershed modeling and identifying the critical time period and pollutions source areas can be valuable for other researchers worldwide.

  2. Solution-Processed CuS NPs as an Inorganic Hole-Selective Contact Material for Inverted Planar Perovskite Solar Cells.

    Science.gov (United States)

    Rao, Haixia; Sun, Weihai; Ye, Senyun; Yan, Weibo; Li, Yunlong; Peng, Haitao; Liu, Zhiwei; Bian, Zuqiang; Huang, Chunhui

    2016-03-01

    Organic-inorganic hybrid perovskite solar cells (PSCs) have drawn worldwide intense research in recent years. Herein, we have first applied another p-type inorganic hole-selective contact material, CuS nanoparticles (CuS NPs), in an inverted planar heterojunction (PHJ) perovskite solar cell. The CuS NP-modification of indium tin oxide (ITO) has successfully tuned the surface work function from 4.9 to 5.1 eV but not affect the surface roughness and transmittance, which can effectively reduce the interfacial carrier injection barrier and facilitate high hole extraction efficiency between the perovskite and ITO layers. After optimization, the maximum power conversion efficiency (PCE) has been over 16% with low J-V hysteresis and excellent stability. Therefore, the low-cost solution-processed and stable CuS NPs would be an alternative interfacial modification material for industrial production in perovskite solar cells.

  3. Fabrication of friction-reducing texture surface by selective laser melting of ink-printed (SLM-IP) copper (Cu) nanoparticles(NPs)

    Science.gov (United States)

    Wang, Xinjian; Liu, Junyan; Wang, Yang; Fu, Yanan

    2017-02-01

    This paper reports a process of selective laser melting of ink-printed (SLM-IP) copper (Cu) nanoparticles(NPs) for the fabrication of full dense Cu friction-reducing texture on the metallic surface in ambient condition. This technique synthesizes pure Cu by chemical reduction route using an organic solvent during laser melting in the atmosphere environment, and provides a flexible additive manufacture approach to form complex friction-reduction texture on the metallic surface. Microtextures of ring and disc arrays have been fabricated on the stainless steel surface by SLM-IP Cu NPs. The friction coefficient has been measured under the lubricating condition of the oil. Disc texture surface (DTS) has a relatively low friction coefficient compared with ring texture surface (RTS), Cu film surface (Cu-FS) and the untreated substrate. The study suggests a further research on SLM-IP approach for complex microstructure or texture manufacturing, possibly realizing its advantage of flexibility.

  4. Functionalization of Fe3O4 NPs by Silanization: Use of Amine (APTES and Thiol (MPTMS Silanes and Their Physical Characterization

    Directory of Open Access Journals (Sweden)

    Silvia Villa

    2016-10-01

    Full Text Available In this paper the results concerning the synthesis of magnetite (Fe3O4 nanoparticles (NPs, their functionalization using silane derivatives, such as (3-Aminopropyltriethoxysilane (APTES and (3-mercaptopropyltrimethoxysilane (MPTMS, and their exhaustive morphological and physical characterization by field emission scanning electron microscopy (FE-SEM with energy dispersion X-ray spectrometer (EDX analysis, AC magnetic susceptibility, UV-VIS and IR spectroscopy, and thermogravimetric (TGA analyses are reported. Two different paths were adopted to achieve the desired functionalization: (1 the direct reaction between the functionalized organo-silane molecule and the surface of the magnetite nanoparticle; and (2 the use of an intermediate silica coating. Finally, the occurrence of both the functionalization with amino and thiol groups has been demonstrated by the reaction with ninhydrin and the capture of Au NPs, respectively.

  5. Stop-and-go, stepwise and "ligand-free" nucleation, nanocrystal growth and formation of Au-NPs in ionic liquids (ILs).

    Science.gov (United States)

    Redel, Engelbert; Walter, Michael; Thomann, Ralf; Hussein, Laith; Krüger, Michael; Janiak, Christoph

    2010-02-21

    Ionic liquids function as a nanosynthetic template for a "ligand-free" stepwise formation of gold nanoparticles (Au-NPs) which can be stopped and resumed at different color steps and Au-NP sizes (between 2.6 and 200 nm median diameter) and which can be reasoned by the DFT-calculation stabilization order: H(2)O (no Au-NP stabilization) IL anion, e.g. BF(4)(-) approximately = PH(3) < citrate

  6. Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells.

    Science.gov (United States)

    Bai, Zhihao; Li, Guiyin; Liang, Jingtao; Su, Jing; Zhang, Yue; Chen, Huaizhou; Huang, Yong; Sui, Weiguo; Zhao, Yongxiang

    2016-08-15

    A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0×10(-8)M to 3.0×10(-6)M with a correlation coefficient of R(2)=0.9968 and with logarithm of H2O2 concentration from 6.0×10(-6)M to 1.0×10(-2)M with a correlation coefficient of R(2)=0.9887, the low detection limit of 20nM was obtained at the signal/noise (S/N) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells.

  7. Toward Quantifying the Impact of Atmospheric Forcing on Arctic Sea Ice Variability Using the NPS 1/12 Degree Pan-Arctic Coupled Ice-Ocean Model

    Science.gov (United States)

    2010-03-01

    Mode NAME Naval Postgraduate School Modeling Effort NAO North Atlantic Oscillation NAVFOR Navy Forces NPS Naval Postgraduate School NSPD...Iceland, as well as non-Arctic nations, China , Japan, South Korea and the European Union are equally ready to preserve their rights to the resources in the...determining speed and direction of the wind and ice. (Note the North Atlantic Oscillation ( NAO ) is similar to that of the AO, or the Northern Annular

  8. Fabrication of highly sensitive and selective nanocomposite film based on CuNPs/fullerene-C60/MWCNTs: An electrochemical nanosensor for trace recognition of paracetamol.

    Science.gov (United States)

    Brahman, Pradeep Kumar; Suresh, Lakkavarapu; Lokesh, Veeramacheneni; Nizamuddin, Syed

    2016-04-21

    Designing an electrochemical sensor for versatile clinical applications is a sophisticated task and how dedicatedly functionalized composite materials can perform on this stage is a challenge for today and tomorrow's Nanoscience and Nanotechnology. In the present work, we demonstrate a new strategy for the development of novel electrochemical sensor based on catalytic nanocomposite film. Fullerene-C60 and multi-walled carbon nanotubes (MWCNTs) were dropped on the pre-treated carbon paste electrode (CPE) and copper nanoparticles (CuNPs) electrochemically deposited on the modified CPE to form nanocomposite film of CuNPs/C60/MWCNTs/CPE. In this work, an electrochemical method based on square wave voltammetry (SWV) employing CuNPs/C60/MWCNTs/CPE has been presented for the recognition and determination of paracetamol (PT). Developed electrochemical sensor was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronocoulometry. The composite film made the fabricated sensor to display high sensitivity and good selectivity for PT detection. The influence of the optimization parameters such as pH, accumulation time, deposition potential, scan rate and effect of loading of composite mixture of C60-MWCNTs and CuNPs on the electrochemical performance of the sensor were evaluated. A linear range from 4.0 × 10(-9) to 4.0 × 10(-7) M for PT detection was obtained with a detection limit of 7.3 × 10(-11) M. The fabricated sensor was successfully applied to the detection of PT in biological samples with good recovery ranging from 99.21 to 103%.

  9. A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA–AuNPs probe

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Dan; Yan, Yurong; Lei, Pinhua; Shen, Bo [Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China); Cheng, Wei [Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China); The Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016 (China); Ju, Huangxian [Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China); State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); Ding, Shijia, E-mail: dingshijia@163.com [Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)

    2014-10-10

    A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA–AuNPs probe. - Highlights: • This paper presented a novel sensing strategy for the rapid and ultrasensitive detection for Salmonella. • Combination of rolling circle amplification and DNA–AuNPs probe is the first time for Salmonella electrochemical detection. • The method displayed excellent sensitivity and specificity for detection of Salmonella. • The fabricated biosensor was successfully applied to detect Salmonella in milk samples. - Abstract: A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA–AuNPs probe. The target DNA could be specifically captured by probe 1 on the sensing interface. Then the circularization mixture was added to form a typical sandwich structure. In the presence of dNTPs and phi29 DNA polymerase, the RCA was initiated to produce micrometer-long single-strand DNA. Finally, the detection probe (DNA–AuNPs) could recognize RCA product to produce enzymatic electrochemical signal. Under optimal conditions, the calibration curve of synthetic target DNA had good linearity from 10 aM to 10 pM with a detection limit of 6.76 aM (S/N = 3). The developed method had been successfully applied to detect Salmonella as low as 6 CFU mL{sup −1} in real milk sample. This proposed strategy showed great potential for clinical diagnosis, food safety and environmental monitoring.

  10. Probing of Ehrlich ascites carcinoma cell using in situ aggregates of Au-NPs as SERS label created by plasmon exciting hybrid- TEM*11 laser mode

    Science.gov (United States)

    Kumar, R.; Mehta, D. S.; Saraswati, S.; Shakher, C.

    2012-02-01

    Apart from commonly employed target-specific labeling/adsorption of antibodies over Au-NPs surface for the creation of localized aggregates, an alternative approach using optical tweezers (OT) driven by hybrid-TEM*11 mode has been devised and exploited for in vitro detection of Ehrlich ascites carcinoma cells (EAC) relying on enhanced scattering. Intra-cavity generated spatially featured asymmetric (SFA) laser beam (λ = 532 nm) has effected simultaneous trapping of mice-EAC cells and in-situ crowd/assembly of incubated Au-NPs/small gold nano-aggregates (created from two or more individual Au-NPs). Relatively larger focus spot created by tightly focused SFA beam than frequently employed Gaussian-mode in OT has offered an extended working area and hence dilute heating has taken care of EAC cells. GNA improves significantly the sensitivity of diagnostics relying on scattered light and the safety and efficacy of therapeutic nanotechnologies for the diseases of cancer and vascular system in medicine.

  11. Antioxidant and antigenotoxic properties of CeO2 NPs and cerium sulphate: Studies with Drosophila melanogaster as a promising in vivo model.

    Science.gov (United States)

    Alaraby, Mohamed; Hernández, Alba; Annangi, Balasubramanyam; Demir, Esref; Bach, Jordi; Rubio, Laura; Creus, Amadeu; Marcos, Ricard

    2015-01-01

    Although in vitro approaches are the most used for testing the potential harmful effects of nanomaterials, in vivo studies produce relevant information complementing in vitro data. In this context, we promote the use of Drosophila melanogaster as a suitable in vivo model to characterise the potential risks associated to nanomaterials exposure. The main aim of this study was to evaluate different biological effects associated to cerium oxide nanoparticles (Ce-NPs) and cerium (IV) sulphate exposure. The end-points evaluated were egg-to-adult viability, particles uptake through the intestinal barrier, gene expression and intracellular reactive oxygen species (ROS) production by haemocytes, genotoxicity and antigenotoxicity. Transmission electron microscopy images showed internalisation of Ce-NPs by the intestinal barrier and haemocytes, and significant expression of Hsp genes was detected. In spite of these findings, neither toxicity nor genotoxicity related to both forms of cerium were observed. Interestingly, Ce-NPs significantly reduced the genotoxic effect of potassium dichromate and the intracellular ROS production. No morphological malformations were detected after larvae treatment. This study highlights the importance of D. melanogaster as animal model in the study of the different biological effects caused by nanoparticulated materials, at the time that shows its usefulness to study the role of the intestinal barrier in the transposition of nanomaterials entering via ingestion.

  12. Dairy Streptococcus thermophilus improves cell viability of Lactobacillus brevis NPS-QW-145 and its γ-aminobutyric acid biosynthesis ability in milk.

    Science.gov (United States)

    Wu, Qinglong; Law, Yee-Song; Shah, Nagendra P

    2015-08-06

    Most high γ-aminobutyric acid (GABA) producers are Lactobacillus brevis of plant origin, which may be not able to ferment milk well due to its poor proteolytic nature as evidenced by the absence of genes encoding extracellular proteinases in its genome. In the present study, two glutamic acid decarboxylase (GAD) genes, gadA and gadB, were found in high GABA-producing L. brevis NPS-QW-145. Co-culturing of this organism with conventional dairy starters was carried out to manufacture GABA-rich fermented milk. It was observed that all the selected strains of Streptococcus thermophilus, but not Lactobacillus delbrueckii subsp. bulgaricus, improved the viability of L. brevis NPS-QW-145 in milk. Only certain strains of S. thermophilus improved the gadA mRNA level in L. brevis NPS-QW-145, thus enhanced GABA biosynthesis by the latter. These results suggest that certain S. thermophilus strains are highly recommended to co-culture with high GABA producer for manufacturing GABA-rich fermented milk.

  13. Various types of semiconductor photocatalysts modified by CdTe QDs and Pt NPs for toluene photooxidation in the gas phase under visible light

    Science.gov (United States)

    Marchelek, M.; Grabowska, E.; Klimczuk, T.; Lisowski, W.; Zaleska-Medynska, A.

    2017-01-01

    A novel synthesis process was used to prepare TiO2 microspheres, TiO2 P-25, SrTiO3 and KTaO3 decorated by CdTe QDs and/or Pt NPs. The effect of semiconductor matrix, presence of CdTe QDs and/or Pt NPs on the semiconductor surface as well as deposition technique of Pt NPs (photodeposition or radiolysis) on the photocatalytic activity were investigated. The as-prepared samples were characterized by X-ray powder diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) with energy-dispersive X-ray (EDX) spectroscopy, scanning electron microscopy (SEM), photoluminescence spectrometry (PL), Fourier transform infrared (FT-IR) and Raman spectra, diffuse reflectance spectroscopy (DRS) and BET surface area analysis. The photocatalytic decomposition of toluene in gas phase, activated by light-emitting diodes (LEDs), with the CdTe/Pt nanoparticles-modified TiO2 microspheres, P25, SrTiO3 and KTaO3 semiconductors was investigated under UV-vis and visible irradiation.The results showed that the photoactivity depends on semiconductor matrix. The highest photoactivity under Vis light was observed for KTaO3/CdTe-Pt(R) sample (56% of toluene was decompose after 30 min of irradiation). The efficiency of the most active sample was 3 times higher than result for P25 and two times higher than for unmodified KTaO3.

  14. Apoferritin-templated biosynthesis of manganese nanoparticles and investigation of direct electron transfer of MnNPs-HsAFr at modified glassy carbon electrode.

    Science.gov (United States)

    Rafipour, Ronak; Kashanian, Soheila; Hashemi, Sadegh; Omidfar, Kobra; Ezzati Nazhad Dolatabadi, Jafar

    2017-01-01

    Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl