WorldWideScience

Sample records for non-periodic housekeeping file

  1. Housekeeping. An Approach to Housekeeping Training.

    Science.gov (United States)

    Hotel and Catering Industry Training Board, Wembley (England).

    This booklet examines the training required by staff employed in housekeeping departments in the hotel and catering industry. It details specifications of particular tasks--baths/cloakrooms; service pantries and utility rooms; beds; furniture/fittings; floors/walls and ceilings; carpets/upholstery/soft furnishings; linen handling; linen room work;…

  2. Agency-Hired Hotel Housekeepers

    Science.gov (United States)

    Sanon, Marie-Anne V.

    2014-01-01

    Hotel housekeepers experience unique workplace hazards and characteristics that increase their risks for poor health outcomes. Today’s agency-hiring practices may further marginalize hotel housekeepers and negatively impact their health. Yet the impact of such hiring practices on the health of this vulnerable worker group remains unexplored. This article presents the debate regarding agency-hiring practices and how these practices may influence the health and well-being of hotel housekeepers. Implications for occupational health nurses are also discussed. PMID:24512722

  3. Hotel housekeeping work influences on hypertension management.

    Science.gov (United States)

    Sanon, Marie-Anne

    2013-12-01

    Characteristics of hotel housekeeping work increase the risk for hypertension development. Little is known about the influences of such work on hypertension management. For this qualitative study, 27 Haitian immigrant hotel housekeepers from Miami-Dade County, FL were interviewed. Interview transcripts were analyzed with the assistance of the Atlas.ti software for code and theme identification. Influences of hotel housekeeping work on hypertension management arose both at the individual and system levels. Factors at the individual level included co-worker dynamics and maintenance of transmigrant life. Factors at the system level included supervisory support, workload, work pace, and work hiring practices. No positive influences were reported for workload and hiring practices. Workplace interventions may be beneficial for effective hypertension management among hotel housekeepers. These work influences must be considered when determining effective methods for hypertension management among hotel housekeepers. © 2013 Wiley Periodicals, Inc.

  4. Hotel Housekeeping Work Influences on Hypertension Management

    Science.gov (United States)

    Sanon, Marie-Anne

    2013-01-01

    Background Characteristics of hotel housekeeping work increase the risk for hypertension development. Little is known about the influences of such work on hypertension management. Methods For this qualitative study, 27 Haitian immigrant hotel housekeepers from Miami-Dade County, FL were interviewed. Interview transcripts were analyzed with the assistance of the Atlas. ti software for code and theme identification. Results Influences of hotel housekeeping work on hypertension management arose both at the individual and system levels. Factors at the individual level included co-worker dynamics and maintenance of transmigrant life. Factors at the system level included supervisory support, workload, work pace, and work hiring practices. No positive influences were reported for workload and hiring practices. Conclusions Workplace interventions may be beneficial for effective hypertension management among hotel housekeepers. These work influences must be considered when determining effective methods for hypertension management among hotel housekeepers. PMID:23775918

  5. ORGANIZATION OF BUSINESS IN HOTEL HOUSEKEEPING

    Directory of Open Access Journals (Sweden)

    Ivica Batinić

    2015-01-01

    Full Text Available Hotel housekeeping department is represented in every organizational structure of the hotel, and its size and structure depends on the type and size of the hotel, basic offer and contents, category of the hotel, the level of usage and the duration of the hotel business. The primary role of hotel housekeeping is cleaning and maintenance of hotel units and rooms, and the selection of the head hotel housekeeper, who will successfully lead the housekeeping department is extremely important for a successful business, recognition and reputation of the hotel. In addition to higher or university education and language skills, the head housekeeper must have the management skills related to planning, organizing, leading and controlling, as well as general and professional knowledge of psychology of work and hygiene. The head hotel housekeeper, as a hotel manager, organizes, directs and controls the work of hotel housekeeping. It is responsible for the accuracy of the occupancy rate report, preparation of rooms and other hotel units, to achieve the highest level of quality of hotel services, training of employees, for the application of standards and for continuous application of business policy of the hotel

  6. Evidence based selection of housekeeping genes.

    Directory of Open Access Journals (Sweden)

    Hendrik J M de Jonge

    Full Text Available For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes is required. It is known that commonly used housekeeping genes (e.g. ACTB, GAPDH, HPRT1, and B2M vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e.g. RPS13, RPL27, RPS20 and OAZ1 with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were present in the top 50 of the most stable expressed genes. In addition, using 2,543 diverse mouse gene array samples we were able to confirm the enhanced stability of the candidate novel housekeeping genes in another mammalian species. Therefore, the identified novel candidate housekeeping genes seem to be the most appropriate choice for normalizing gene expression data.

  7. The housekeeper and the professor a novel

    CERN Document Server

    Ogawa, Yoko

    2010-01-01

    He is a brilliant math Professor with a peculiar problem—ever since a traumatic head injury, he has lived with only eighty minutes of short-term memory. She is an astute young Housekeeper—with a ten-year-old son—who is hired to care for the Professor. And every morning, as the Professor and the Housekeeper are introduced to each other anew, a strange and beautiful relationship blossoms between them. Though he cannot hold memories for long (his brain is like a tape that begins to erase itself every eighty minutes), the Professor's mind is still alive with elegant equations from the past. And the numbers, in all of their articulate order, reveal a sheltering and poetic world to both the Housekeeper and her young son. The Professor is capable of discovering connections between the simplest of quantities—like the Housekeeper's shoe size—and the universe at large, drawing their lives ever closer and more profoundly together, even as his memory slips away. Yoko Ogawa's The Housekeeper and the Professo...

  8. Housekeeping category corrective action unit work plan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    The purpose of this Corrective Action Unit (CAU) Work Plan is to provide a strategy to be used by the US Department of Energy Nevada Operations Office (DOE/NV), the US Department of Defense (DoD) Defense Special Weapons Agency (DSWA) (formerly the Defense Nuclear Agency), and contractor personnel for conducting corrective actions at the Nevada Test Site (NTS) and Nevada off-site locations including the Tonopah Test Range (TTR), the Project Shoal Area, and the Central Nevada Test Area. This Work Plan applies to housekeeping category CAUs already listed in the Federal Facility Agreement and Consent Order (FFACO) Appendices (FFACO, 1996) as well as newly identified Corrective Action Sites (CASs) that will follow the housekeeping process.

  9. KMOS housekeeping electronics and its functions

    Science.gov (United States)

    Bezawada, Nagaraja; Woodward, Brian; Rees, Phil

    2008-07-01

    KMOS is a second generation near infrared multi-object spectrograph instrument for the VLT. It is a highly complex astronomical instrument with over 60 cryo-mechanisms deploying pickoff arms, moving filter wheels and detector focus stages. The instrument houses three identical sections each consisting of 8 pickoff arms, 2 filter wheels, 8 integral field units feeding a spectrograph and its detector systems. The housekeeping electronics provides a semi-automation of the cryostat functions such as pump down, cool down and warm up sequences, vacuum and temperature measurement and control etc. The infrastructure electronics is responsible for the safe operation of the instrument. It monitors the various cryostat conditions, takes automatic corrective actions under faulty conditions and raises alarms when a manual intervention is needed. This semi-automation design is aimed at not only minimising the risk of instrument damage, but also takes into account the safety of instrument manual handling. This paper describes the design of the instrument infrastructure electronics and details its functions such as semi-automation of the cryostat procedures, housekeeping diagnostics, automatic corrective actions under faulty conditions, scheme of alarm and warnings, detector thermal protection etc. and presents the associated interfaces to the control electronics and the cable co-rotator.

  10. Perceived workplace mistreatment: Case of Latina hotel housekeepers.

    Science.gov (United States)

    Hsieh, Yu-Chin Jerrie; Sönmez, Sevil; Apostolopoulos, Yorghos; Lemke, Michael Kenneth

    2017-01-01

    Latina hotel housekeepers' social class, gender, race/ethnicity, nationality, and United States immigration status render them particularly vulnerable to workplace mistreatment. We sought to reveal the array of policy- and interpersonal-related mistreatment experienced by Latina hotel housekeepers in the southeastern United States employed at 75 local hotels which included 4-star, 3-star, 2-star, and 1-star properties. This ethnographic study involved 27 in-depth interviews with Latina hotel housekeepers. Using semi-structured in-depth interview guides, participants were interviewed until collected data reached saturation. Data were coded to explore themes and relationships for the housekeepers' work environments, and thick descriptions of these environments were developed. Participants ranged in work experience from 1 to 15 years, with all but one unable to reach full-time status, and were paid between $7.25 and $8.00 per hour. Policy-related phenomena, such as low pay, lack of paid sick leave or overtime, and absence of appropriate cleaning tools or protective equipment were all perceived as forms of mistreatment by Latina hotel housekeepers. Interpersonal mistreatment in the form of supervisor favoritism, unfair work assignments, biased allocation of cleaning supplies, disrespect, and verbal abuse due to ethnicity was also perceived. Latina hotel housekeepers endure mistreatment that impacts their psychosocial and physical occupational health. We provide recommendations to minimize workplace mistreatment and improve well-being of Latina hotel housekeepers.

  11. HOTEL AND MOTEL HOUSEKEEPING AIDE, A SUGGESTED TRAINING PROGRAM.

    Science.gov (United States)

    Office of Education (DHEW), Washington, DC.

    A LOCAL TRAINING PROGRAM TO PREPARE HOTEL AND MOTEL HOUSEKEEPING AIDES CAN BE DEVELOPED FROM RESOURCE MATERIAL IN THIS GUIDE. PROGRAM OBJECTIVES ARE TO PREPARE TRAINEES TO PERFORM THE JOBS INVOLVED IN KEEPING HOTEL OR MOTEL ROOMS CLEAN, TO FOLLOW CORRECT PROCEDURES IN USING EQUIPMENT AND SUPPLIES REQUIRED IN CARING FOR BEDROOMS AND BATHROOMS, AND…

  12. How do 'housekeeping' genes control organogenesis?--Unexpected new findings on the role of housekeeping genes in cell and organ differentiation.

    Science.gov (United States)

    Tsukaya, Hirokazu; Byrne, Mary E; Horiguchi, Gorou; Sugiyama, Munetaka; Van Lijsebettens, Mieke; Lenhard, Michael

    2013-01-01

    In recent years, an increasing number of mutations in what would appear to be 'housekeeping genes' have been identified as having unexpectedly specific defects in multicellular organogenesis. This is also the case for organogenesis in seed plants. Although it is not surprising that loss-of-function mutations in 'housekeeping' genes result in lethality or growth retardation, it is surprising when (1) the mutant phenotype results from the loss of function of a 'housekeeping' gene and (2) the mutant phenotype is specific. In this review, by defining housekeeping genes as those encoding proteins that work in basic metabolic and cellular functions, we discuss unexpected links between housekeeping genes and specific developmental processes. In a surprising number of cases housekeeping genes coding for enzymes or proteins with functions in basic cellular processes such as transcription, post-transcriptional modification, and translation affect plant development.

  13. Housekeeping and tissue-specific genes in mouse tissues

    Directory of Open Access Journals (Sweden)

    St-Amand Jonny

    2007-05-01

    Full Text Available Abstract Background This study aims to characterize the housekeeping and tissue-specific genes in 15 mouse tissues by using the serial analysis of gene expression (SAGE strategy which indicates the relative level of expression for each transcript matched to the tag. Results Here, we identified constantly expressed housekeeping genes, such as eukaryotic translation elongation factor 2, which is expressed in all tissues without significant difference in expression levels. Moreover, most of these genes were not regulated by experimental conditions such as steroid hormones, adrenalectomy and gonadectomy. In addition, we report previously postulated housekeeping genes such as peptidyl-prolyl cis-trans isomerase A, glyceraldehyde-3-phosphate dehydrogenase and beta-actin, which are expressed in all the tissues, but with significant difference in their expression levels. We have also identified genes uniquely detected in each of the 15 tissues and other tissues from public databases. Conclusion These identified housekeeping genes could represent appropriate controls for RT-PCR and northern blot when comparing the expression levels of genes in several tissues. The results reveal several tissue-specific genes highly expressed in testis and pituitary gland. Furthermore, the main function of tissue-specific genes expressed in liver, lung and bone is the cell defence, whereas several keratins involved in cell structure function are exclusively detected in skin and vagina. The results from this study can be used for example to target a tissue for agent delivering by using the promoter of tissue-specific genes. Moreover, this study could be used as basis for further researches on physiology and pathology of these tissues.

  14. Advanced Computational Methods for Optimization of Non Periodic Inspection Intervals for Aging Infrastructure

    Science.gov (United States)

    2017-01-05

    4-1 Statistical results for different number of sample ( non -periodic, true value) Number of sample Number of inspections Number of failure Number...distribution unlimited. 51 Table 5-1 Inspection schedule of non -periodic scheme by conditional probability (true value) Inspection no. Inspection time...50 elements Fig. 5-1 Reliability for the non -periodic inspection by conditional probability DISTRIBUTION A. Approved for public release

  15. De-regulation of common housekeeping genes in hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Wurmbach Elisa

    2007-07-01

    Full Text Available Abstract Background Tumorigenesis is associated with changes in gene expression and involves many pathways. Dysregulated genes include "housekeeping" genes that are often used for normalization for quantitative real-time RT-PCR (qPCR, which may lead to unreliable results. This study assessed eight stages of hepatitis C virus (HCV induced hepatocellular carcinoma (HCC to search for appropriate genes for normalization. Results Gene expression profiles using microarrays revealed differential expression of most "housekeeping" genes during the course of HCV-HCC, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH and beta-actin (ACTB, genes frequently used for normalization. QPCR reactions confirmed the regulation of these genes. Using them for normalization had strong effects on the extent of differential expressed genes, leading to misinterpretation of the results. Conclusion As shown here in the case of HCV-induced HCC, the most constantly expressed gene is the arginine/serine-rich splicing factor 4 (SFRS4. The utilization of at least two genes for normalization is robust and advantageous, because they can compensate for slight differences of their expression when not co-regulated. The combination of ribosomal protein large 41 (RPL41 and SFRS4 used for normalization led to very similar results as SFRS4 alone and is a very good choice for reference in this disease as shown on four differentially expressed genes.

  16. Comparison of Characteristics of Periodic and Non-Periodic Defected Ground Structures

    Institute of Scientific and Technical Information of China (English)

    LI Yuan; LI Huancai; DING Ronglin

    2006-01-01

    The filter characteristic of defected ground structure(DGS)is analyzed and the equivalent circuit of C-shaped DGS is extracted. The characteristics of non-periodic and periodic DGS with different dimensions are compared. Then the DGS is simulated and optimized with software, and the circuit board is manufactured and measured. The non-periodic structure is simple in structure and small in size and ripple compared with the periodic structure. Though the stop band of the non-periodic structure is narrow, it can meet the requirement of application. The C-shaped structure with two stop bands can select frequency in a special band.

  17. Identification of optimal housekeeping genes for examination of gene expression in bovine corpus luteum.

    Science.gov (United States)

    Rekawiecki, Robert; Rutkowska, Joanna; Kotwica, Jan

    2012-12-01

    The selection of proper housekeeping genes for studies requiring genes expression normalization is an important step in the appropriate interpretation of results. The expression of housekeeping genes is regulated by many factors including age, gender, type of tissue or disease. The aim of the study was to identify optimal housekeeping genes in the corpus luteum obtained from cyclic or pregnant cows. The mRNA expression of thirteen housekeeping genes: C2orf29, SUZ12, TBP, TUBB2B, ZNF131, HPRT1, 18s RNA, GAPDH, SF3A1, SDHA, MRPL12, B2M and ACTB was measured by Real-time PCR. Range of cycle threshold (C(t)) values of the tested genes varied between 12 and 30 cycles, and 18s RNA had the highest coefficient of variation, whereas C2orf29 had the smallest coefficient. GeNorm software demonstrated C2orf29 and TBP as the most stable and 18s RNA and B2M as the most unstable housekeeping genes. Using the proposed cut-off value (0.15), no more than two of the best GeNorm housekeeping genes are proposed to be used in studies requiring gene expression normalization. NormFinder software demonstrated C2orf29 and SUZ12 as the best and 18s RNA and B2M as the worst housekeeping genes. The study indicates that selection of housekeeping genes may essentially affect the quality of the gene expression results.

  18. Hybrid simulation of whistler excitation by electron beams in two-dimensional non-periodic domains

    Energy Technology Data Exchange (ETDEWEB)

    Woodroffe, J.R., E-mail: woodrofj@erau.edu; Streltsov, A.V., E-mail: streltsa@erau.edu

    2014-11-01

    We present a two-dimensional hybrid fluid-PIC scheme for the simulation of whistler wave excitation by relativistic electron beams. This scheme includes a number of features which are novel to simulations of this type, including non-periodic boundary conditions and fresh particle injection. Results from our model suggest that non-periodicity of the simulation domain results in the development of fundamentally different wave characteristics than are observed in periodic domains.

  19. Translational selection in human: More pronounced in housekeeping genes

    KAUST Repository

    Ma, Lina

    2014-07-10

    Background: Translational selection is a ubiquitous and significant mechanism to regulate protein expression in prokaryotes and unicellular eukaryotes. Recent evidence has shown that translational selection is weakly operative in highly expressed genes in human and other vertebrates. However, it remains unclear whether translational selection acts differentially on human genes depending on their expression patterns.Results: Here we report that human housekeeping (HK) genes that are strictly defined as genes that are expressed ubiquitously and consistently in most or all tissues, are under stronger translational selection.Conclusions: These observations clearly show that translational selection is also closely associated with expression pattern. Our results suggest that human HK genes are more efficiently and/or accurately translated into proteins, which will inevitably open up a new understanding of HK genes and the regulation of gene expression.Reviewers: This article was reviewed by Yuan Yuan, Baylor College of Medicine; Han Liang, University of Texas MD Anderson Cancer Center (nominated by Dr Laura Landweber) Eugene Koonin, NCBI, NLM, NIH, United States of America Sandor Pongor, International Centre for Genetic Engineering and biotechnology (ICGEB), Italy. © 2014 Ma et al.; licensee BioMed Central Ltd.

  20. Non-periodic molecular dynamics simulations of coarse grained lipid bilayer in water

    DEFF Research Database (Denmark)

    Kotsalis, E. M.; Hanasaki, I.; Walther, Jens Honore

    2010-01-01

    We present a multiscale algorithm that couples coarse grained molecular dynamics (CGMD) with continuum solver. The coupling requires the imposition of non-periodic boundary conditions on the coarse grained Molecular Dynamics which, when not properly enforced, may result in spurious fluctuations...... of the material properties of the system represented by CGMD. In this paper we extend a control algorithm originally developed for atomistic simulations [3], to conduct simulations involving coarse grained water molecules without periodic boundary conditions. We demonstrate the applicability of our method...... in simulating more complex systems by performing a non-periodic Molecular Dynamics simulation of a DPPC lipid in liquid coarse grained water....

  1. The NSL complex regulates housekeeping genes in Drosophila.

    Directory of Open Access Journals (Sweden)

    Kin Chung Lam

    Full Text Available MOF is the major histone H4 lysine 16-specific (H4K16 acetyltransferase in mammals and Drosophila. In flies, it is involved in the regulation of X-chromosomal and autosomal genes as part of the MSL and the NSL complexes, respectively. While the function of the MSL complex as a dosage compensation regulator is fairly well understood, the role of the NSL complex in gene regulation is still poorly characterized. Here we report a comprehensive ChIP-seq analysis of four NSL complex members (NSL1, NSL3, MBD-R2, and MCRS2 throughout the Drosophila melanogaster genome. Strikingly, the majority (85.5% of NSL-bound genes are constitutively expressed across different cell types. We find that an increased abundance of the histone modifications H4K16ac, H3K4me2, H3K4me3, and H3K9ac in gene promoter regions is characteristic of NSL-targeted genes. Furthermore, we show that these genes have a well-defined nucleosome free region and broad transcription initiation patterns. Finally, by performing ChIP-seq analyses of RNA polymerase II (Pol II in NSL1- and NSL3-depleted cells, we demonstrate that both NSL proteins are required for efficient recruitment of Pol II to NSL target gene promoters. The observed Pol II reduction coincides with compromised binding of TBP and TFIIB to target promoters, indicating that the NSL complex is required for optimal recruitment of the pre-initiation complex on target genes. Moreover, genes that undergo the most dramatic loss of Pol II upon NSL knockdowns tend to be enriched in DNA Replication-related Element (DRE. Taken together, our findings show that the MOF-containing NSL complex acts as a major regulator of housekeeping genes in flies by modulating initiation of Pol II transcription.

  2. The NSL Complex Regulates Housekeeping Genes in Drosophila

    Science.gov (United States)

    Raja, Sunil Jayaramaiah; Holz, Herbert; Luscombe, Nicholas M.; Manke, Thomas; Akhtar, Asifa

    2012-01-01

    MOF is the major histone H4 lysine 16-specific (H4K16) acetyltransferase in mammals and Drosophila. In flies, it is involved in the regulation of X-chromosomal and autosomal genes as part of the MSL and the NSL complexes, respectively. While the function of the MSL complex as a dosage compensation regulator is fairly well understood, the role of the NSL complex in gene regulation is still poorly characterized. Here we report a comprehensive ChIP–seq analysis of four NSL complex members (NSL1, NSL3, MBD-R2, and MCRS2) throughout the Drosophila melanogaster genome. Strikingly, the majority (85.5%) of NSL-bound genes are constitutively expressed across different cell types. We find that an increased abundance of the histone modifications H4K16ac, H3K4me2, H3K4me3, and H3K9ac in gene promoter regions is characteristic of NSL-targeted genes. Furthermore, we show that these genes have a well-defined nucleosome free region and broad transcription initiation patterns. Finally, by performing ChIP–seq analyses of RNA polymerase II (Pol II) in NSL1- and NSL3-depleted cells, we demonstrate that both NSL proteins are required for efficient recruitment of Pol II to NSL target gene promoters. The observed Pol II reduction coincides with compromised binding of TBP and TFIIB to target promoters, indicating that the NSL complex is required for optimal recruitment of the pre-initiation complex on target genes. Moreover, genes that undergo the most dramatic loss of Pol II upon NSL knockdowns tend to be enriched in DNA Replication–related Element (DRE). Taken together, our findings show that the MOF-containing NSL complex acts as a major regulator of housekeeping genes in flies by modulating initiation of Pol II transcription. PMID:22723752

  3. Escher degree of non-periodic L-tilings by 2 prototiles

    CERN Document Server

    Ahara, Kazushi; Ojiri, Anno

    2012-01-01

    For a given tiling of the euclidean plane ${\\bf E}^2$, we call the degree of freedom of perturbed edges of prototiles {\\it escher degree}. In this paper we consider non-periodic L-tilings by 2 prototiles and obtain the escher degree of them.

  4. Gene Ontology based housekeeping gene selection for RNA-seq normalization.

    Science.gov (United States)

    Chen, Chien-Ming; Lu, Yu-Lun; Sio, Chi-Pong; Wu, Guan-Chung; Tzou, Wen-Shyong; Pai, Tun-Wen

    2014-06-01

    RNA-seq analysis provides a powerful tool for revealing relationships between gene expression level and biological function of proteins. In order to identify differentially expressed genes among various RNA-seq datasets obtained from different experimental designs, an appropriate normalization method for calibrating multiple experimental datasets is the first challenging problem. We propose a novel method to facilitate biologists in selecting a set of suitable housekeeping genes for inter-sample normalization. The approach is achieved by adopting user defined experimentally related keywords, GO annotations, GO term distance matrices, orthologous housekeeping gene candidates, and stability ranking of housekeeping genes. By identifying the most distanced GO terms from query keywords and selecting housekeeping gene candidates with low coefficients of variation among different spatio-temporal datasets, the proposed method can automatically enumerate a set of functionally irrelevant housekeeping genes for pratical normalization. Novel and benchmark testing RNA-seq datasets were applied to demostrate that different selections of housekeeping gene lead to strong impact on differential gene expression analysis, and compared results have shown that our proposed method outperformed other traditional approaches in terms of both sensitivity and specificity. The proposed mechanism of selecting appropriate houskeeping genes for inter-dataset normalization is robust and accurate for differential expression analyses. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Validation of housekeeping genes for studying differential gene expression in the bovine myometrium.

    Science.gov (United States)

    Rekawiecki, Robert; Kowalik, Magdalena K; Kotwica, Jan

    2013-12-01

    The aim of this study was to determine the steady-state expression of 13 selected housekeeping genes in the myometrium of cyclic and pregnant cows. Cells taken from bovine myometrium on days 1-5, 6-10, 11-16 and 17-20 of the oestrous cycle and in weeks 3-5, 6-8 and 9-12 of pregnancy were used. Reverse transcribed RNA was amplified in real-time PCR using designed primers. Reaction efficiency was determined with the Linreg programme. The geNorm and NormFinder programmes were used to select the best housekeeping genes. They calculate the expression stability factor for each used housekeeping gene with the smallest value for most stably expressed genes. According to geNorm, the most stable housekeeping genes in the myometrium were C2orf29, TPB and TUBB2B, while the least stably expressed genes were 18S RNA, HPRT1 and GAPDH. NormFinder identified the best genes in the myometrium as C2orf29, MRPL12 and TBP, while the worst genes were 18S RNA, B2M and SF3A1. Differences in stability factors between the two programmes may also indicate that the physiological status of the female, e.g. pregnancy, affects the stability of expression of housekeeping genes. The different expression stability of housekeeping genes did not affect progesterone receptor expression but it could be important if small differences in gene expression were measured between studies.

  6. Quasi-periodic and Non-periodic Waves in (2+1)-Dimensional Nonlinear Systems

    Institute of Scientific and Technical Information of China (English)

    TANG Xiao-Yan; LOU Sen-Yue

    2005-01-01

    New exact quasi-periodic and non-periodic solutions for the (2 + 1)-dimensional nonlinear systems are studied by means of the multi-linear variable separation approach (MLVSA) and the Jacobi elliptic functions with the space-time-dependent modulus. Though the result is valid for all the MLVSA solvable models, it is explicitly shown for the long-wave and short-wave interaction model.

  7. Comparision of Incidental Reflection From Containerized Maintenance/Housekeeping Solutions and One Inch of Water

    Energy Technology Data Exchange (ETDEWEB)

    Chapman, Bryan Scott [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); MacQuigg, Michael Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Wysong, Andrew Russell [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-12-15

    This document addresses the incidental reflector reactivity worth of containerized maintenance/housekeeping fluids for use in PF-4 at Los Alamos National Laboratory (LANL). The intent of the document is to analyze containerized maintenance/housekeeping fluids which will be analyzed as water that may be present under normal conditions of an operation. The reactivity worth is compared to the reactivity worth due to I-inch of close-fitting 4n water reflection and I-inch of close-fitting radial water reflection. Both have been used to bound incidental reflection by 2-liter bottles in criticality safety evaluations. The conclusion is that, when the maintenance/housekeeping fluids are containerized the reactivity increase from a configuration which is bounding of normal conditions (up to eight bottles modeled with 2-liters of solution at varying diameter) is bound by I-inch of close fitting 4n water relection.

  8. Work Conditions and Health and Well-Being of Latina Hotel Housekeepers.

    Science.gov (United States)

    Hsieh, Yu-Chin; Apostolopoulos, Yorghos; Sönmez, Sevil

    2016-06-01

    Hotel housekeepers are exposed to a plethora of disproportionately high work-induced hazards that can lead to adverse health consequences. Latina hotel housekeepers are rendered particularly vulnerable to elevated occupational hazards and resultant health strains due to their socioeconomic status, immigration status, language barriers, and lack of access to healthcare services. The findings from the 27 interviews with Latina hotel housekeepers indicated that the interviewees were exposed to physical, chemical, and social hazards in the workplace and suffered musculoskeletal injuries. In terms of psychological wellness, the time pressure of cleaning rooms quickly and work-related stress stemming from workplace mistreatment emerged as major work-related stressors. Recommendations are made for the introduction of multilevel interventions designed to prevent work-related injuries and illnesses and to promote healthier workplaces.

  9. Non-Periodic Finite-Element Formulation of Orbital-Free Density Functional Theory

    Energy Technology Data Exchange (ETDEWEB)

    Gavini, V; Knap, J; Bhattacharya, K; Ortiz, M

    2006-10-06

    We propose an approach to perform orbital-free density functional theory calculations in a non-periodic setting using the finite-element method. We consider this a step towards constructing a seamless multi-scale approach for studying defects like vacancies, dislocations and cracks that require quantum mechanical resolution at the core and are sensitive to long range continuum stresses. In this paper, we describe a local real space variational formulation for orbital-free density functional theory, including the electrostatic terms and prove existence results. We prove the convergence of the finite-element approximation including numerical quadratures for our variational formulation. Finally, we demonstrate our method using examples.

  10. Multiscale method for Oseen problem in porous media with non-periodic grain patterns

    CERN Document Server

    Muljadi, Bagus Putra

    2016-01-01

    Accurate prediction of the macroscopic flow parameters needed to describe flow in porous media relies on a good knowledge of flow field distribution at a much smaller scale---in the pore spaces. The extent of the inertial effect in the pore spaces can not be underestimated yet is often ignored in large-scale simulations of fluid flow. We present a multiscale method for solving Oseen's approximation of incompressible flow in the pore spaces amid non-periodic grain patterns. The method is based on the multiscale finite element method (MsFEM [Hou and Wu, 1997]) and is built in the vein of Crouzeix-Raviart elements [Crouzeix and Raviart, 1973]. Simulations of inertial flow in highly non-periodic settings are conducted and presented. Convergence studies in terms of numerical errors relative to the reference solution are given to demonstrate the accuracy of our method. The weakly enforced continuity across coarse element edges is shown to maintain accurate solutions in the vicinity of the grains without the need fo...

  11. Optimization of non-periodic plasmonic light-trapping layers for thin-film solar cells.

    Science.gov (United States)

    Pala, Ragip A; Liu, John S Q; Barnard, Edward S; Askarov, Daulet; Garnett, Erik C; Fan, Shanhui; Brongersma, Mark L

    2013-01-01

    Non-periodic arrangements of nanoscale light scatterers allow for the realization of extremely effective broadband light-trapping layers for solar cells. However, their optimization is challenging given the massive number of degrees of freedom. Brute-force, full-field electromagnetic simulations are computationally too time intensive to identify high-performance solutions in a vast design space. Here we illustrate how a semi-analytical model can be used to quickly identify promising non-periodic spatial arrangements of nanoscale scatterers. This model only requires basic knowledge of the scattering behaviour of a chosen nanostructure and the waveguiding properties of the semiconductor layer in a cell. Due to its simplicity, it provides new intuition into the ideal amount of disorder in high-performance light-trapping layers. Using simulations and experiments, we demonstrate that arrays of nanometallic stripes featuring a limited amount of disorder, for example, following a quasi-periodic or Fibonacci sequence, can substantially enhance solar absorption over perfectly periodic and random arrays.

  12. Optimization of non-periodic plasmonic light-trapping layers for thin-film solar cells

    Science.gov (United States)

    Pala, Ragip A.; Liu, John S. Q.; Barnard, Edward S.; Askarov, Daulet; Garnett, Erik C.; Fan, Shanhui; Brongersma, Mark L.

    2013-07-01

    Non-periodic arrangements of nanoscale light scatterers allow for the realization of extremely effective broadband light-trapping layers for solar cells. However, their optimization is challenging given the massive number of degrees of freedom. Brute-force, full-field electromagnetic simulations are computationally too time intensive to identify high-performance solutions in a vast design space. Here we illustrate how a semi-analytical model can be used to quickly identify promising non-periodic spatial arrangements of nanoscale scatterers. This model only requires basic knowledge of the scattering behaviour of a chosen nanostructure and the waveguiding properties of the semiconductor layer in a cell. Due to its simplicity, it provides new intuition into the ideal amount of disorder in high-performance light-trapping layers. Using simulations and experiments, we demonstrate that arrays of nanometallic stripes featuring a limited amount of disorder, for example, following a quasi-periodic or Fibonacci sequence, can substantially enhance solar absorption over perfectly periodic and random arrays.

  13. Is Cleanliness Next to Godliness? The Role of Housekeeping in Impression Formation

    Science.gov (United States)

    Harris, Paul B.; Sachau, Daniel

    2005-01-01

    We conducted a study to determine if the cleanliness of an apartment would affect observer impressions of the resident. Participants (210 female and 126 male undergraduate students) read a story in which a character's apartment was described as clean or dirty or in which no information about housekeeping was provided. For each condition, half the…

  14. Adaptations of Homemaking Skills for the Aged: Housekeeping. Teacher's Manual and Participant's Manual.

    Science.gov (United States)

    Pestle, Ruth E.; Harris, Nancy C.

    This manual is designed for use with adult or secondary school home economics students to enhance their awareness of the tasks and physical limitations faced by aged homemakers, and to identify possible adaptations for making the housekeeping task easier and safer for older adults. The five most common physical limitations experienced by the aged…

  15. 9 CFR 3.84 - Cleaning, sanitization, housekeeping, and pest control.

    Science.gov (United States)

    2010-01-01

    ..., and pest control. 3.84 Section 3.84 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION..., sanitization, housekeeping, and pest control. (a) Cleaning of primary enclosures. Excreta and food waste must... from becoming soiled, and to reduce disease hazards, insects, pests, and odors. Dirt floors,...

  16. 9 CFR 3.11 - Cleaning, sanitization, housekeeping, and pest control.

    Science.gov (United States)

    2010-01-01

    ..., and pest control. 3.11 Section 3.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION..., sanitization, housekeeping, and pest control. (a) Cleaning of primary enclosures. Excreta and food waste must... contained in the primary enclosures, and to reduce disease hazards, insects, pests and odors. When steam...

  17. The sensitivity of neurons with non-periodic activity to sympathetic stimulation in rat injured dorsal root ganglion

    Institute of Scientific and Technical Information of China (English)

    Hong-Jun YANG; San-Jue HU; Pu-Lin GONG; Jian-Hong DUAN

    2006-01-01

    Objective The relationship between firing pattern and sensitivity of neurons was studied in chronically compressed dorsal root ganglion (DRG) neurons and the Hindmarsh-Rose (HR) neuronal model. Methods Spontaneous activities from single fibers of chronically compressed DRG neurons in rats were recorded, and divided into periodic and non-periodic firing patterns. The sensitivity of the two kinds of firing pattern neuron to sympathetic stimulation (SS)was compared. Result It was found that 27.3% of periodic firing neurons and 93.2% of non-periodic firing neurons responded to SS respectively ( periodic vs non-periodic, P < 0.01 ). The responses to SS with different stimulation time were greater non-periodic firing neurons than periodic firing neurons (P < 0.01 ). The non-periodic firing neurons obviously responded to SS. After the firing pattern of these neurons transformed to periodic firing pattern, their responses to SS disappeared or decreased obviously. The HR neuronal model exhibited a significantly greater response to perturbation in non-periodic (chaotic) firing pattern than in periodic firing pattern. Conclusion The non-periodic firing neurons with deterministic chaos are more sensitive to external stimuli than the periodic firing neurons.

  18. White Spot Syndrome Virus infection in Penaeus monodon is facilitated by housekeeping molecules

    Indian Academy of Sciences (India)

    Vinayak Biradar; Santosh Narwade; Mandar Paingankar; Deepti Deobagkar

    2013-12-01

    White Spot Syndrome Virus (WSSV) is a major pathogen in shrimp aquaculture, and its rampant spread has resulted in great economic loss. Identification of host cellular proteins interacting with WSSV will help in unravelling the repertoire of host proteins involved in WSSV infection. In this study, we have employed one-dimensional and two-dimension virus overlay protein binding assay (VOPBA) followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the host proteins of Penaeus monodon that could interact with WSSV. The VOPBA results suggest that WSSV interacted with housekeeping proteins such as heat shock protein 70, ATP synthase subunit , phosphopyruvate hydratase, allergen Pen m 2, glyceraldehyde-3-phosphate dehydrogenase, sarcoplasmic calcium-binding protein, actin and 14-3-3-like protein. Our findings suggest that WSSV exploits an array of housekeeping proteins for its transmission and propagation in P. monodon.

  19. Soft Topographic Maps for Clustering and Classifying Bacteria Using Housekeeping Genes

    Directory of Open Access Journals (Sweden)

    Massimo La Rosa

    2011-01-01

    Full Text Available The Self-Organizing Map (SOM algorithm is widely used for building topographic maps of data represented in a vectorial space, but it does not operate with dissimilarity data. Soft Topographic Map (STM algorithm is an extension of SOM to arbitrary distance measures, and it creates a map using a set of units, organized in a rectangular lattice, defining data neighbourhood relationships. In the last years, a new standard for identifying bacteria using genotypic information began to be developed. In this new approach, phylogenetic relationships of bacteria could be determined by comparing a stable part of the bacteria genetic code, the so-called “housekeeping genes.” The goal of this work is to build a topographic representation of bacteria clusters, by means of self-organizing maps, starting from genotypic features regarding housekeeping genes.

  20. Understanding the Role of Housekeeping and Stress-Related Genes in Transcription-Regulatory Networks

    Science.gov (United States)

    Heath, Allison; Kavraki, Lydia; Balázsi, Gábor

    2008-03-01

    Despite the increasing number of completely sequenced genomes, much remains to be learned about how living cells process environmental information and respond to changes in their surroundings. Accumulating evidence indicates that eukaryotic and prokaryotic genes can be classified in two distinct categories that we will call class I and class II. Class I genes are housekeeping genes, often characterized by stable, noise resistant expression levels. In contrast, class II genes are stress-related genes and often have noisy, unstable expression levels. In this work we analyze the large scale transcription-regulatory networks (TRN) of E. coli and S. cerevisiae and preliminary data on H. sapien. We find that stable, housekeeping genes (class I) are preferentially utilized as transcriptional inputs while stress related, unstable genes (class II) are utilized as transcriptional integrators. This might be the result of convergent evolution that placed the appropriate genes in the appropriate locations within transcriptional networks according to some fundamental principles that govern cellular information processing.

  1. Special Features In Turbulent Mixing. Comparison between Periodic and Non Periodic Case

    Directory of Open Access Journals (Sweden)

    Mihai Costescu

    2006-12-01

    Full Text Available After hundreds of years of stability study, the problems of flow kinematics are far from complete solving. A modern theory appears in this field: the mixing theory. Its mathematical methods and techniques developed the significant relation between turbulence and chaos. The turbulence is an important feature of dynamic systems with few freedom degrees, the so-called far from equilibrium systems. These are widespread between the models of excitable media.Studying a mixing for a flow implies the analysis of successive stretching and folding phenomena for its particles, the influence of parameters and initial conditions. In the previous works, the study of the 3D non-periodic models exhibited a quite complicated behavior, involving some significant events - the so-called rare events. The variation of parameters had a great influence on the length and surface deformations. The 2D (periodic case is simpler, but significant events can also issue for irrational values of the length and surface versors, as was the situation in 3D case.The comparison between 2D and 3D case revealed interesting properties; therefore a modified 2D (periodic model is tested. The numerical simulations were realized in MapleVI, for searching special mathematical events. Continuing this work both from analytical and numeric standpoint would relieve useful properties for the turbulent mixing. A proximal target is to test some special functions for the periodic model, and to study the behavior of the structures realized by the model.

  2. Statistical Approach to Diffraction of Periodic and Non-Periodic Crystals—Review

    Directory of Open Access Journals (Sweden)

    Radoslaw Strzalka

    2016-08-01

    Full Text Available In this paper, we show the fundamentals of statistical method of structure analysis. Basic concept of a method is the average unit cell, which is a probability distribution of atomic positions with respect to some reference lattices. The distribution carries complete structural information required for structure determination via diffraction experiment regardless of the inner symmetry of diffracting medium. The shape of envelope function that connects all diffraction maxima can be derived as the Fourier transform of a distribution function. Moreover, distributions are sensitive to any disorder introduced to ideal structure—phonons and phasons. The latter are particularly important in case of quasicrystals. The statistical method deals very well with phason flips and may be used to redefine phasonic Debye-Waller correction factor. The statistical approach can be also successfully applied to the peak’s profile interpretation. It will be shown that the average unit cell can be equally well applied to a description of Bragg peaks as well as other components of diffraction pattern, namely continuous and singular continuous components. Calculations performed within statistical method are equivalent to the ones from multidimensional analysis. The atomic surface, also called occupation domain, which is the basic concept behind multidimensional models, acquires physical interpretation if compared to average unit cell. The statistical method applied to diffraction analysis is now a complete theory, which deals equally well with periodic and non-periodic crystals, including quasicrystals. The method easily meets also any structural disorder.

  3. Focused Wave Properties Based on A High Order Spectral Method with A Non-Periodic Boundary

    Institute of Scientific and Technical Information of China (English)

    李金宣; 柳淑学

    2015-01-01

    In this paper, a numerical model is developed based on the High Order Spectral (HOS) method with a non-periodic boundary. A wave maker boundary condition is introduced to simulate wave generation at the incident boundary in the HOS method. Based on the numerical model, the effects of wave parameters, such as the assumed focused amplitude, the central frequency, the frequency bandwidth, the wave amplitude distribution and the directional spreading on the surface elevation of the focused wave, the maximum generated wave crest, and the shifting of the focusing point, are numerically investigated. Especially, the effects of the wave directionality on the focused wave properties are emphasized. The numerical results show that the shifting of the focusing point and the maximum crest of the wave group are dependent on the amplitude of the focused wave, the central frequency, and the wave amplitude distribution type. The wave directionality has a definite effect on multidirectional focused waves. Generally, it can even out the difference between the simulated wave amplitude and the amplitude expected from theory and reduce the shifting of the focusing points, implying that the higher order interaction has an influence on wave focusing, especially for 2D wave. In 3D wave groups, a broader directional spreading weakens the higher nonlinear interactions.

  4. Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus

    OpenAIRE

    Becker Sven; Scharsack Joern P; Hibbeler Sascha

    2008-01-01

    Abstract Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research. In this context, the expression of immune response genes in teleost fish has been surveyed for scientific and commercial purposes. Despite the fact that it was shown in teleostei and other taxa that the gene for beta-actin is not the most stably expressed housekeeping gene (HKG), depending on the tissue and experimental treatmen...

  5. Effects of a work injury prevention program for housekeeping in the hotel industry.

    Science.gov (United States)

    Landers, Merrill; Maguire, Lynn

    2004-01-01

    The aim of this retrospective study was to determine the effectiveness of a work injury prevention program in the housekeeping department of a hotel. Studies have validated the use of different injury prevention strategies to decrease the incidence of work-related injuries. Few studies, however, have reported the efficacy of an on-site work injury prevention program by a physical therapist. In 1995, implementation of a work injury prevention program by a physical therapist to 50 housekeeping supervisors, 60 house persons and 340 guest room attendants at a large hotel began. This program included a detailed work risk analysis of the work environment, development of job descriptions, identification of injury-related problematic work situations, and implementation of a job specific supervisor-training program. Supervisor, house person and guest room attendant training was also conducted at the end of 1995 and the beginning of 1997. Data of injury reports in 1995, 1996, and 1997 were analyzed to determine the results of the program. There was a reduction in total injury claims, total medical expenses, total lost work time and total restricted duty time. These results demonstrate the cost effectiveness of implementing a work injury prevention program for housekeeping guest room attendants in the hotel industry. Copyright 2004 IOS Press

  6. Comparative analysis of housekeeping and tissue-selective genes in human based on network topologies and biological properties.

    Science.gov (United States)

    Yang, Lei; Wang, Shiyuan; Zhou, Meng; Chen, Xiaowen; Zuo, Yongchun; Sun, Dianjun; Lv, Yingli

    2016-06-01

    Housekeeping genes are genes that are turned on most of the time in almost every tissue to maintain cellular functions. Tissue-selective genes are predominantly expressed in one or a few biologically relevant tissue types. Benefitting from the massive gene expression microarray data obtained over the past decades, the properties of housekeeping and tissue-selective genes can now be investigated on a large-scale manner. In this study, we analyzed the topological properties of housekeeping and tissue-selective genes in the protein-protein interaction (PPI) network. Furthermore, we compared the biological properties and amino acid usage between these two gene groups. The results indicated that there were significant differences in topological properties between housekeeping and tissue-selective genes in the PPI network, and housekeeping genes had higher centrality properties and may play important roles in the complex biological network environment. We also found that there were significant differences in multiple biological properties and many amino acid compositions. The functional genes enrichment and subcellular localizations analysis was also performed to investigate the characterization of housekeeping and tissue-selective genes. The results indicated that the two gene groups showed significant different enrichment in drug targets, disease genes and toxin targets, and located in different subcellular localizations. At last, the discriminations between the properties of two gene groups were measured by the F-score, and expression stage had the most discriminative index in all properties. These findings may elucidate the biological mechanisms for understanding housekeeping and tissue-selective genes and may contribute to better annotate housekeeping and tissue-selective genes in other organisms.

  7. Non-periodic one-dimensional ideal conductors and integrable turbulence

    Energy Technology Data Exchange (ETDEWEB)

    Zakharov, Dmitry V. [Courant Institute of Mathematical Sciences, New York University, 251 Mercer Street, New York, NY, 10012 (United States); Zakharov, Vladimir E. [Department of Mathematics, University of Arizona, Tucson, AZ, 85791 (United States); Dyachenko, Sergey A., E-mail: sdyachen@math.uiuc.edu [Department of Mathematics, University of Illinois, Urbana-Champaign, IL, 61801 (United States)

    2016-12-01

    Highlights: • An efficient procedure for construction of non-periodic, non-vanishing reflectionless potentials is presented. • The analytical procedure is reinforced by numerical simulation that presents some of these potentials. • The present work is a key ingredient for the study of integrable turbulence and statistical description of “solitonic gas”. - Abstract: To relate the motion of a quantum particle to the properties of the potential is a fundamental problem of physics, which is far from being solved. Can a medium with a potential which is neither periodic nor quasi-periodic be a conductor? That question seems to have been never addressed, despite being both interesting and having practical importance. Here we propose a new approach to the spectral problem of the one-dimensional Schrödinger operator with a bounded potential. We construct a wide class of potentials having a spectrum consisting of the positive semiaxis and finitely many bands on the negative semiaxis. These potentials, which we call primitive, are reflectionless for positive energy and in general are neither periodic nor quasi-periodic. Moreover, they can be stochastic, and yet allow ballistic transport, and thus describe one-dimensional ideal conductors. Primitive potentials also generate a new class of solutions of the KdV hierarchy. Stochastic primitive potentials describe integrable turbulence, which is important for hydrodynamics and nonlinear optics. We construct the potentials by numerically solving a system of singular integral equations. We hypothesize that finite-gap potentials are a subclass of primitive potentials, and prove this in the case of one-gap potentials.

  8. Needlestick and sharps injuries among housekeeping workers in hospitals of Shiraz, Iran

    Directory of Open Access Journals (Sweden)

    Lakbala Parvin

    2012-06-01

    Full Text Available Abstract Background Needlestick and sharps injuries (NSSIs are one of the major risk factors for blood-borne infections (BBPs at healthcare facilities. This study examines the current prevalence of NSSIs among housekeeping workers engaged in the handling and disposal of biomedical waste (BMW at government and private hospitals in Shiraz, Iran, and furthermore, explores strategies for preventing these injuries. Findings Using a cross-sectional study design, NSSI's and associated protective measures for housekeeping workers throughout hospitals in Shiraz were evaluated from 2009 onwards. Using a questionnaire, data was collected for 92 workers who had engaged directly with BMW. Data was analyzed using Chi-square, student t-test and where appropriate, SPSS version 12. 90.2 % of housekeeping workers were warned of the dangers associated with waste, 87.5 % in government and 93.2 % in private hospitals (P = 0.0444. 83.7 % had attended educational programs on biomedical waste (BMW management and injury prevention at their hospital in the preceding year. 16.3 % had not been trained in biomedical waste management (P = 0.0379 and 88.9 % had a sufficient supply of safety wear. Conclusions NSSIs are a common risk factor for infection among health care workers within hospitals in Iran. For the effective prevention of these injuries, health boards and hospital trusts need to formulate strategies to improve the working conditions of health care workers, discourage the excessive use of injections, and increase their adherence to universal precautions.

  9. Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae.

    Directory of Open Access Journals (Sweden)

    Chunxiao Yang

    Full Text Available Quantitative real-time PCR (qRT-PCR is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory. In this study, ten housekeeping genes, including beta-actin (Actin , elongation factor 1 α (EF1A , glyceralde hyde-3-phosphate dehydrogenase (GAPDH , ribosomal protein L13 (RPL13 , ribosomal protein 49 (RP49 , α-tubulin (Tubulin , vacuolar-type H+-ATPase (v-ATPase , succinate dehydrogenase subunit A (SDHA , 28S ribosomal RNA (28S , and 18S ribosomal RNA (18S from the two-spotted spider mite, Tetranychus urticae, were selected as the candidate reference genes. Four algorithms, geNorm, Normfinder, BestKeeper, and the ΔCt method, were used to evaluate the performance of these candidates as endogenous controls across different developmental stages. In addition, RefFinder, which integrates the above-mentioned software tools, provided the overall ranking of the stability/suitability of these candidate reference genes. Among them, PRL13 and v-ATPase were the two most stable housekeeping genes across different developmental stages. This work is the first step toward establishing a standardized qRT-PCR analysis in T. urticae following the MIQE guideline. With the recent release of the T. urticae genome, results from this study provide a critical piece for the subsequent genomics and functional genomics research in this emerging model system.

  10. The characteristics and development status of the control and housekeeping electronics of FRIDA

    Science.gov (United States)

    Díaz, J. J.; Flores-Meza, R.; Sánchez, Beatriz; Patrón, Jesús

    2014-07-01

    FRIDA (inFRared Imager and Dissector for the Adaptive optics system of the Gran Telescopio Canarias) is a diffraction limited instrument that will offer broad and narrow band imaging and integral field spectroscopy with low, intermediate and high spectral resolutions in the 0.9 - 2.5 μm wavelength range. FRIDA will be installed at a Nasmyth focus of GTC, behind the AO system. The characteristics and development status of the Control and Housekeeping Electronics are described in this contribution. FRIDA is a collaborative project between the IAC (Spain), UNAM (México), UCM (Spain) and the UF (Florida), lead by UNAM.

  11. The effects of converting wheels on housekeeping carts in a large urban hotel. Program evaluation.

    Science.gov (United States)

    Intilli, H

    1999-10-01

    Occupational and environmental health nurses can identify the causes of injuries and use analytical skills to show how the prevention of a problem can save the company money and demonstrate a caring attitude from management. Nurses can expand their traditional roles to position themselves as both advocates for the employees and profit enhancers to management as demonstrated by this program evaluation project. Initial outcomes included improved employee morale and reduced soft tissue injuries in hotel housekeeping employees. Changes in the workplace made by a proactive occupational and environmental health nurse and a committed management can reap rewards beneficial for both the employer and the work force.

  12. Developing an Interactive Instructional Compact Disk for the Course of Basic Housekeeping

    Directory of Open Access Journals (Sweden)

    Nurlaila Nurlaila

    2017-04-01

    Full Text Available This research aimed at creating the product based on the concept of learning for basic housekeeping.  The development of interactive media in the form of interactive compact disk (CD was conducted at Universitas Negeri Jakarta. Based on the review from the media expert, the total average score was categorized good i.e. 4,31. Meanwhile, from the material expert, the average score was categorized good i.e. 4,51 with the percentage of 90,28% and categorized as very good. The individual test (one to one and the limited test showed the average scores of 4,13 and 4,09 respectively which were both categorized as good. In the field study, it was tested to 23 college students mutually and the total of average score was very good, i.e. 3,8 with the percentage score of 76 %. It showed that the quality of interactive CD of housekeeping course was very good.

  13. Nurse's Aid And Housekeeping Mobile Robot For Use In The Nursing Home Workplace

    Science.gov (United States)

    Sines, John A.

    1987-01-01

    The large nursing home market has several natural characteristics which make it a good applications area for robotics. The environment is already robot accessible and the work functions require large quantities of low skilled services on a daily basis. In the near future, a commercial opportunity for the practical application of robots is emerging in the delivery of housekeeping services in the nursing home environment. The robot systems will assist in food tray delivery, material handling, and security, and will perform activities such as changing a resident's table side drinking water twice a day, and taking out the trash. The housekeeping work functions will generate cost savings of approximately 22,000 per year, at a cost of 6,000 per year. Technical system challenges center around the artificial intelligence required for the robot to map its own location within the facility, to find objects, and to avoid obstacles, and the development of an energy efficient mechanical lifting system. The long engineering and licensing cycles (7 to 12 years) required to bring this type of product to market make it difficult to raise capital for such a venture.

  14. Comparative analysis of chromatin landscape in regulatory regions of human housekeeping and tissue specific genes

    Directory of Open Access Journals (Sweden)

    Dasgupta Dipayan

    2005-05-01

    Full Text Available Abstract Background Global regulatory mechanisms involving chromatin assembly and remodelling in the promoter regions of genes is implicated in eukaryotic transcription control especially for genes subjected to spatial and temporal regulation. The potential to utilise global regulatory mechanisms for controlling gene expression might depend upon the architecture of the chromatin in and around the gene. In-silico analysis can yield important insights into this aspect, facilitating comparison of two or more classes of genes comprising of a large number of genes within each group. Results In the present study, we carried out a comparative analysis of chromatin characteristics in terms of the scaffold/matrix attachment regions, nucleosome formation potential and the occurrence of repetitive sequences, in the upstream regulatory regions of housekeeping and tissue specific genes. Our data show that putative scaffold/matrix attachment regions are more abundant and nucleosome formation potential is higher in the 5' regions of tissue specific genes as compared to the housekeeping genes. Conclusion The differences in the chromatin features between the two groups of genes indicate the involvement of chromatin organisation in the control of gene expression. The presence of global regulatory mechanisms mediated through chromatin organisation can decrease the burden of invoking gene specific regulators for maintenance of the active/silenced state of gene expression. This could partially explain the lower number of genes estimated in the human genome.

  15. Os afazeres domésticos contam Accounting for housekeeping activities

    Directory of Open Access Journals (Sweden)

    Hildete Pereira de Melo

    2007-12-01

    Full Text Available Este artigo tem como objetivo propor uma mensuração para as atividades realizadas pelas pessoas no interior dos lares, as quais têm enorme importância na reprodução da vida e no bem-estar da sociedade. Esses serviços gerados na execução dos afazeres domésticos, por não estarem associados a uma geração equivalente de renda, são ignorados pela teoria econômica que não os valora e não contabiliza no Produto Interno Bruto (PIB dos países. Uma provável interpretação para esse não-reconhecimento origina-se na histórica discriminação sofrida pelas mulheres nas diversas sociedades, a quem foi delegada a execução dos afazeres domésticos. Desconhecê-los reforça o conceito de invisibilidade, que caracteriza o trabalho doméstico e a inferioridade do papel da mulher na sociedade. Tendo por base os procedimentos usuais de estimativas de bens ou serviços não mensurados por estatísticas econômicas, estatísticas demográficas e sociais originárias da Pesquisa Nacional por Amostra de Domicílios (PNAD que, desde 2001 investiga o tempo gasto na execução de tarefas domésticas, chega-se à conclusão de que, no Brasil, esses afazeres corresponderam, em média, a 11,2% dos PIBs brasileiros do período 2001-2005.This paper proposes a measurement for housekeeping activities, which play an enormous role in the reproduction of life and in the well-being of society. These housekeeping activities are ignored in economic theory, which neither values them nor accounts for them in the Gross Domestic Product measures, as far as they are not associated with an equivalent flow of monetary revenue. A plausible interpretation for this non-consideration derives from the historical discrimination, in most societies against women, to whom the carrying out of housekeeping activities has been delegated. Ignoring housekeeping activities reinforces the concept of invisibility, which characterizes domestic labor and the inferior role of women in

  16. Spectroscopic Evidence of Sporadic Gas Accretion onto the Herbig AE Stars with Non-Periodic Algol-Type Minima

    Science.gov (United States)

    Kozlova, O. V.; Grinin, V. P.; Rostopchina, A. N.

    We present the results of simultaneous spectroscopic and photometric observations for six isolated Herbig Ae stars with non-periodic Algol-like minima: UX Ori, BF Ori, CQ Tau, SV Cep, VX Gas, and WW Vul. In all cases the Ha line has the profile typical for axially symmetric accretion. In the spectra of five stars (CQ Tau is the exception) the strong He I 5876 Å line has been observed in absorption which is not typical of normal A stars. In three cases: UX Ori, VX Cas, and WW Vul, variability of this line is found. We did not observe any correlation between the strength of this line and the brightness of the stars. These observational facts are considered as evidence for gas accretion, which is probably an important property of young stars with non-periodic Algol-type minima.

  17. Identification of reference housekeeping-genes for mRNA expression studies in patients with type 1 diabetes.

    Science.gov (United States)

    Kar, Parmita; Chawla, Himika; Saha, Soma; Tandon, Nikhil; Goswami, Ravinder

    2016-06-01

    Selection of appropriate housekeeping-genes as reference is important in mRNA expression-related experiments. It is more important in diabetes since hyperglycemia per se can influence expression of housekeeping-genes. RNA expression of Glyceraldehyde-3-phosphate-dehydrogenase, β-actin and 18S-ribosomal-RNA, Hypoxanthine-phosphoribosyl-transferase (HPRT), Tyrosine-3-monooxygenase/tryptophan (YHWAZ), β2-microglobin (β2M), TATA-binding-protein (TBP), and Ubiquitin C and cytochrome1 (CYC1) assessed in circulating-lymphocytes-(PBMC) of patients with type-1-diabetes and healthy controls. The stability ('M' value genes required for normalization in qRT-PCR were determined by 'ge-norm software.' Vitamin-D-receptor (VDR) was used as a target gene. All the nine genes tested had sufficient 'M' value in diabetes and healthy controls. However, housekeeping-genes indicated a relatively higher stability of expression in healthy controls in comparison to diabetes. Use of single housekeeping-genes brought gross variation in the calculation of VDR-mRNA copies. The ge-norm software suggested geometric mean of five housekeeping-genes for ideal normalization in diabetes (CYC1, β-actin, YHWAZ, HPRT, and β2M) and only three in controls (CYC1, β-actin, and TBP). HbA1c did not correlate with expression of any of the nine housekeeping-genes. Thus, geometric mean of CYC1, β-actin, YHWAZ, HPRT, and β2M needs to be used for ideal normalization of mRNA in type-1-diabetes. Similar studies are required in other population.

  18. Membrane gene ontology bias in sequencing and microarray obtained by housekeeping-gene analysis.

    Science.gov (United States)

    Zhang, Yijuan; Akintola, Oluwafemi S; Liu, Ken J A; Sun, Bingyun

    2016-01-10

    Microarray (MA) and high-throughput sequencing are two commonly used detection systems for global gene expression profiling. Although these two systems are frequently used in parallel, the differences in their final results have not been examined thoroughly. Transcriptomic analysis of housekeeping (HK) genes provides a unique opportunity to reliably examine the technical difference between these two systems. We investigated here the structure, genome location, expression quantity, microarray probe coverage, as well as biological functions of differentially identified human HK genes by 9 MA and 6 sequencing studies. These in-depth analyses allowed us to discover, for the first time, a subset of transcripts encoding membrane, cell surface and nuclear proteins that were prone to differential identification by the two platforms. We hope that the discovery can aid the future development of these technologies for comprehensive transcriptomic studies. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Overview: membrane traffic in multicellular systems: more than just a housekeeper.

    Science.gov (United States)

    Ohno, Hiroshi

    2006-06-01

    Membrane traffic is a fundamental cellular function by which molecules are transported between organelles in the post-Golgi network. Accumulating evidence supports the notion that membrane traffic is not only indispensable for normal cellular function and maintenance of cellular viability by playing housekeeping roles, but also critical for various functions characteristic of multicellular organisms. This Minireview series will focus on the latter aspects of membrane traffic. The topics discussed are: the pathophysiological impact of clathrin-associated adaptor protein (AP) complexes, the significance of membrane traffic in Alzheimer's disease, regulated exocytosis of insulin, secretory lysosomes in immune response, exosomes in physiology and pathology, viral and mammalian ubiquitin ligases modulating immune response, membrane traffic of bacterial toxins, and containment of bacterial infection by autophagy.

  20. Closure Report for Housekeeping Category Corrective Action Unit 524 Nevada Test Site Nevada

    Energy Technology Data Exchange (ETDEWEB)

    A. T. Urbon

    2000-11-01

    This Closure Report for Corrective Action Unit (CAU) 524 summarizes the disposition of four Corrective Action Sites (CAS) located in Area 25 of the Nevada Test Site, Nevada. The table listed in the report provides a description of each CAS and the status of its associated waste as listed in the ''Federal Facilities Agreement and Consent Order'' (FFACO, 1996). Copies of the Sectored Housekeeping Site Closure Verification Form for each CAS are included as Attachment A. Two of the sites required sampling for waste disposal purposes, CAS 25-22-18 and 25-22-20. The material sampled at these two sites were found to be not hazardous. Results of the sampling are included in Attachment B.

  1. Closure Report for Housekeeping Category Corrective Action Unit 345 Nevada Test Site, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    A. T. Urbon

    2000-11-01

    This Closure Report for Corrective Action Unit (CAU) 345 summarizes the disposition of ten Corrective Action Sites (CAS) located in Areas 2 and 9 of the Nevada Test Site, Nevada. The table listed in the report provides a description of each CAS and the status of its associated waste as listed in the ''Federal Facilities Agreement and Consent Order'' (FFACO, 1996). Copies of the Sectored Housekeeping Site Closure Verification Form for each CAS are included as Attachment A. The battery at CAS 09-24-04 required sampling for waste disposal purposes. The waste was found to be not hazardous. Results of the sampling are included in Attachment B.

  2. Closure Report for Housekeeping Category Corrective Action Unit 524 Nevada Test Site Nevada

    Energy Technology Data Exchange (ETDEWEB)

    A. T. Urbon

    2000-11-01

    This Closure Report for Corrective Action Unit (CAU) 524 summarizes the disposition of four Corrective Action Sites (CAS) located in Area 25 of the Nevada Test Site, Nevada. The table listed in the report provides a description of each CAS and the status of its associated waste as listed in the ''Federal Facilities Agreement and Consent Order'' (FFACO, 1996). Copies of the Sectored Housekeeping Site Closure Verification Form for each CAS are included as Attachment A. Two of the sites required sampling for waste disposal purposes, CAS 25-22-18 and 25-22-20. The material sampled at these two sites were found to be not hazardous. Results of the sampling are included in Attachment B.

  3. Reconstruction of Family-Level Phylogenetic Relationships within Demospongiae (Porifera) Using Nuclear Encoded Housekeeping Genes

    Science.gov (United States)

    Hill, Malcolm S.; Hill, April L.; Lopez, Jose; Peterson, Kevin J.; Pomponi, Shirley; Diaz, Maria C.; Thacker, Robert W.; Adamska, Maja; Boury-Esnault, Nicole; Cárdenas, Paco; Chaves-Fonnegra, Andia; Danka, Elizabeth; De Laine, Bre-Onna; Formica, Dawn; Hajdu, Eduardo; Lobo-Hajdu, Gisele; Klontz, Sarah; Morrow, Christine C.; Patel, Jignasa; Picton, Bernard; Pisani, Davide; Pohlmann, Deborah; Redmond, Niamh E.; Reed, John; Richey, Stacy; Riesgo, Ana; Rubin, Ewelina; Russell, Zach; Rützler, Klaus; Sperling, Erik A.; di Stefano, Michael; Tarver, James E.; Collins, Allen G.

    2013-01-01

    Background Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. Methodology/Principal Findings We generated data from each of the four sponge classes (i.e., Calcarea, Demospongiae, Hexactinellida, and Homoscleromorpha), but focused on family-level relationships within demosponges. With data for 21 newly sampled families, our Maximum Likelihood and Bayesian-based approaches recovered previously phylogenetically defined taxa: Keratosap, Myxospongiaep, Spongillidap, Haploscleromorphap (the marine haplosclerids) and Democlaviap. We found conflicting results concerning the relationships of Keratosap and Myxospongiaep to the remaining demosponges, but our results strongly supported a clade of Haploscleromorphap+Spongillidap+Democlaviap. In contrast to hypotheses based on mitochondrial genome and ribosomal data, nuclear housekeeping gene data suggested that freshwater sponges (Spongillidap) are sister to Haploscleromorphap rather than part of Democlaviap. Within Keratosap, we found equivocal results as to the monophyly of Dictyoceratida. Within Myxospongiaep, Chondrosida and Verongida were monophyletic. A well-supported clade within Democlaviap, Tetractinellidap, composed of all sampled members of Astrophorina and Spirophorina (including the only lithistid in our analysis), was consistently revealed as the sister group to all other members of Democlaviap. Within Tetractinellidap, we did not recover monophyletic Astrophorina or Spirophorina. Our results also reaffirmed the monophyly of order Poecilosclerida (excluding Desmacellidae and Raspailiidae), and polyphyly of Hadromerida and Halichondrida. Conclusions/Significance These results, using an

  4. Bypassing hazard of housekeeping genes: Their evaluation in rat granule neurons treated with cerebrospinal fluid of Multiple Sclerosis subjects

    Directory of Open Access Journals (Sweden)

    Deepali eMathur

    2015-09-01

    Full Text Available Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s based on stably expressed housekeeping genes is crucial in individual experimental conditions to obtain accurate results. In multiple sclerosis (MS, several gene expression studies have been undertaken, however, the suitability of housekeeping genes to express stably in this disease is not yet explored. Recent research suggests that their expression level may vary under different experimental conditions. Hence it is indispensible to evaluate their expression stability to accurately normalize target gene transcripts. The present study aims to evaluate the expression stability of seven housekeeping genes in rat granule neurons treated with cerebrospinal fluid of MS patients. The selected reference genes were quantified by real time PCR and their expression stability was assessed using GeNorm and NormFinder algorithms. Both methods reported transferrin receptor (Tfrc and microglobulin beta-2 (B2m the most stable genes whereas beta-actin (ActB and glyceraldehyde-3-phosphate-dehydrogenase (Gapdh the most fluctuated ones. Altogether our data demonstrate the significance of pre-validation of housekeeping genes for accurate normalization and indicates Tfrc and B2m as best endogenous controls in MS. ActB and Gapdh are not recommended in gene expression studies related to the current one.

  5. Candidate qRT-PCR reference genes for barley that demonstrate better stability than traditional housekeeping genes

    Science.gov (United States)

    Gene transcript expression analysis is a useful tool for correlating gene activity with plant phenotype. For these studies, an appropriate reference gene is necessary to quantify the expression of target genes. Classic housekeeping genes have often been used for this purpose, but may not be consis...

  6. Applications of non-periodic multilayer optics for high-resolution x-ray microscopes below 30 keVa)

    Science.gov (United States)

    Troussel, Ph.; Dennetiere, D.; Rousseau, A.; Darbon, S.; Høghøj, P.; Hedacq, S.; Krumrey, M.

    2012-10-01

    Multilayer mirrors with enhanced bandwidth were developed with special performances for dense plasma diagnostics and mainly for high spatial resolution x-ray imaging. The multilayer coatings are designed to provide broadband x-ray reflectance at low grazing incidence angles. They are deposited onto toroidal mirror substrates. Our research is directed at the development of non-periodic (depth graded) W/Si multilayer specifically designed for use in the 1 to 30 keV photon energy band. First, we present a study for a 5 to 22 keV x-ray spectral window at 0.45° grazing angle. The goal is to obtain a high and constant reflectivity. Second, we have modeled a broadband mirror coating for harder x-rays in the range from 10 to 30 keV, with a non-periodic structure containing 300 W/SiC layers with periods in the range from 0.8 to 4 nm, designed for 0.35° grazing incidence angle.

  7. File sharing

    NARCIS (Netherlands)

    van Eijk, N.

    2011-01-01

    File sharing’ has become generally accepted on the Internet. Users share files for downloading music, films, games, software etc. In this note, we have a closer look at the definition of file sharing, the legal and policy-based context as well as enforcement issues. The economic and cultural impact

  8. Definition, conservation and epigenetics of housekeeping and tissue-enriched genes

    Directory of Open Access Journals (Sweden)

    Johnson Jason M

    2009-06-01

    Full Text Available Abstract Background Housekeeping genes (HKG are constitutively expressed in all tissues while tissue-enriched genes (TEG are expressed at a much higher level in a single tissue type than in others. HKGs serve as valuable experimental controls in gene and protein expression experiments, while TEGs tend to represent distinct physiological processes and are frequently candidates for biomarkers or drug targets. The genomic features of these two groups of genes expressed in opposing patterns may shed light on the mechanisms by which cells maintain basic and tissue-specific functions. Results Here, we generate gene expression profiles of 42 normal human tissues on custom high-density microarrays to systematically identify 1,522 HKGs and 975 TEGs and compile a small subset of 20 housekeeping genes which are highly expressed in all tissues with lower variance than many commonly used HKGs. Cross-species comparison shows that both the functions and expression patterns of HKGs are conserved. TEGs are enriched with respect to both segmental duplication and copy number variation, while no such enrichment is observed for HKGs, suggesting the high expression of HKGs are not due to high copy numbers. Analysis of genomic and epigenetic features of HKGs and TEGs reveals that the high expression of HKGs across different tissues is associated with decreased nucleosome occupancy at the transcription start site as indicated by enhanced DNase hypersensitivity. Additionally, we systematically and quantitatively demonstrated that the CpG islands' enrichment in HKGs transcription start sites (TSS and their depletion in TEGs TSS. Histone methylation patterns differ significantly between HKGs and TEGs, suggesting that methylation contributes to the differential expression patterns as well. Conclusion We have compiled a set of high quality HKGs that should provide higher and more consistent expression when used as references in laboratory experiments than currently used

  9. Reconstruction of family-level phylogenetic relationships within Demospongiae (Porifera) using nuclear encoded housekeeping genes.

    Science.gov (United States)

    Hill, Malcolm S; Hill, April L; Lopez, Jose; Peterson, Kevin J; Pomponi, Shirley; Diaz, Maria C; Thacker, Robert W; Adamska, Maja; Boury-Esnault, Nicole; Cárdenas, Paco; Chaves-Fonnegra, Andia; Danka, Elizabeth; De Laine, Bre-Onna; Formica, Dawn; Hajdu, Eduardo; Lobo-Hajdu, Gisele; Klontz, Sarah; Morrow, Christine C; Patel, Jignasa; Picton, Bernard; Pisani, Davide; Pohlmann, Deborah; Redmond, Niamh E; Reed, John; Richey, Stacy; Riesgo, Ana; Rubin, Ewelina; Russell, Zach; Rützler, Klaus; Sperling, Erik A; di Stefano, Michael; Tarver, James E; Collins, Allen G

    2013-01-01

    Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. We generated data from each of the four sponge classes (i.e., Calcarea, Demospongiae, Hexactinellida, and Homoscleromorpha), but focused on family-level relationships within demosponges. With data for 21 newly sampled families, our Maximum Likelihood and Bayesian-based approaches recovered previously phylogenetically defined taxa: Keratosa(p), Myxospongiae(p), Spongillida(p), Haploscleromorpha(p) (the marine haplosclerids) and Democlavia(p). We found conflicting results concerning the relationships of Keratosa(p) and Myxospongiae(p) to the remaining demosponges, but our results strongly supported a clade of Haploscleromorpha(p)+Spongillida(p)+Democlavia(p). In contrast to hypotheses based on mitochondrial genome and ribosomal data, nuclear housekeeping gene data suggested that freshwater sponges (Spongillida(p)) are sister to Haploscleromorpha(p) rather than part of Democlavia(p). Within Keratosa(p), we found equivocal results as to the monophyly of Dictyoceratida. Within Myxospongiae(p), Chondrosida and Verongida were monophyletic. A well-supported clade within Democlavia(p), Tetractinellida(p), composed of all sampled members of Astrophorina and Spirophorina (including the only lithistid in our analysis), was consistently revealed as the sister group to all other members of Democlavia(p). Within Tetractinellida(p), we did not recover monophyletic Astrophorina or Spirophorina. Our results also reaffirmed the monophyly of order Poecilosclerida (excluding Desmacellidae and Raspailiidae), and polyphyly of Hadromerida and Halichondrida. These results, using an independent nuclear gene set

  10. Reconstruction of family-level phylogenetic relationships within Demospongiae (Porifera using nuclear encoded housekeeping genes.

    Directory of Open Access Journals (Sweden)

    Malcolm S Hill

    Full Text Available BACKGROUND: Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. METHODOLOGY/PRINCIPAL FINDINGS: We generated data from each of the four sponge classes (i.e., Calcarea, Demospongiae, Hexactinellida, and Homoscleromorpha, but focused on family-level relationships within demosponges. With data for 21 newly sampled families, our Maximum Likelihood and Bayesian-based approaches recovered previously phylogenetically defined taxa: Keratosa(p, Myxospongiae(p, Spongillida(p, Haploscleromorpha(p (the marine haplosclerids and Democlavia(p. We found conflicting results concerning the relationships of Keratosa(p and Myxospongiae(p to the remaining demosponges, but our results strongly supported a clade of Haploscleromorpha(p+Spongillida(p+Democlavia(p. In contrast to hypotheses based on mitochondrial genome and ribosomal data, nuclear housekeeping gene data suggested that freshwater sponges (Spongillida(p are sister to Haploscleromorpha(p rather than part of Democlavia(p. Within Keratosa(p, we found equivocal results as to the monophyly of Dictyoceratida. Within Myxospongiae(p, Chondrosida and Verongida were monophyletic. A well-supported clade within Democlavia(p, Tetractinellida(p, composed of all sampled members of Astrophorina and Spirophorina (including the only lithistid in our analysis, was consistently revealed as the sister group to all other members of Democlavia(p. Within Tetractinellida(p, we did not recover monophyletic Astrophorina or Spirophorina. Our results also reaffirmed the monophyly of order Poecilosclerida (excluding Desmacellidae and Raspailiidae, and polyphyly of Hadromerida and Halichondrida. CONCLUSIONS

  11. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

    Directory of Open Access Journals (Sweden)

    Callard Gloria V

    2008-11-01

    Full Text Available Abstract Background Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR, normalized to an internal reference ("housekeeping" gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping genes are known to vary with experimental conditions. To identify housekeeping genes that are stably expressed under different experimental conditions, and thus suitable as normalizers for QPCR in zebrafish, the present study evaluated the expression of eight commonly used housekeeping genes as a function of stage and hormone/toxicant exposure during development, and by tissue type and sex in adult fish. Results QPCR analysis was used to quantify mRNA levels of bactin1, tubulin alpha 1(tuba1, glyceraldehyde-3-phosphate dehydrogenase (gapdh, glucose-6-phosphate dehydrogenase (g6pd, TATA-box binding protein (tbp, beta-2-microglobulin (b2m, elongation factor 1 alpha (elfa, and 18s ribosomal RNA (18s during development (2 – 120 hr postfertilization, hpf; in different tissue types (brain, eye, liver, heart, muscle, gonads of adult males and females; and after treatment of embryos/larvae (24 – 96 hpf with commonly used vehicles for administration and agents that represent known environmental endocrine disruptors. All genes were found to have some degree of variability under the conditions tested here. Rank ordering of expression stability using geNorm analysis identified 18s, b2m, and elfa as most stable during development and across tissue types, while gapdh, tuba1, and tpb were the most variable. Following chemical treatment, tuba1, bactin1, and elfa were the most stably expressed whereas tbp, 18s, and b2m were the least stable. Data also revealed sex differences that are gene- and tissue-specific, and treatment effects that are gene-, vehicle- and ligand-specific. When the accuracy of QPCR analysis was tested using

  12. A condition-based maintenance policy with non-periodic inspections for a two-unit series system

    Energy Technology Data Exchange (ETDEWEB)

    Castanier, B. [IRCCyN/Ecole des Mines de Nantes, Departement Automatique et Productique, 4 rue Alfred Kastler, F-44307 Nantes (France)]. E-mail: bruno.castanier@emn.fr; Grall, A. [Universite de Technologie de Troyes, ISTIT-CNRS FRE 2732, Equipe Modelisation et Surete des Systemes, 12, rue Marie Curie, BP 2060-10010 Troyes Cedex (France)]. E-mail: antoine.grall@utt.fr; Berenguer, C. [Universite de Technologie de Troyes, ISTIT-CNRS FRE 2732, Equipe Modelisation et Surete des Systemes, 12, rue Marie Curie, BP 2060-10010 Troyes Cedex (France)]. E-mail: christophe.berenguer@utt.fr

    2005-01-01

    This paper considers a condition-based maintenance policy for a two-unit deteriorating system. Each unit is subject to gradual deterioration and is monitored by sequential non-periodic inspections. It can be maintained by good as new preventive or corrective replacements. Every inspection or replacement entails a set-up cost and a component-specific unit cost but if actions on the two components are combined, the set-up cost is charged only once. A parametric maintenance decision framework is proposed to coordinate inspection/replacement of the two components and minimize the long-run maintenance cost of the system. A stochastic model is developed on the basis of the semi-regenerative properties of the maintained system state and the associated cost model is used to assess and optimize the performance of the maintenance model. Numerical experiments emphasize the interest of a control of the operation groupings.

  13. Outflow boundary conditions for 3D simulations of non-periodic blood flow and pressure fields in deformable arteries

    CERN Document Server

    Vignon-Clementel, Irene; Jansen, K E; Taylor, C A; 10.1080/10255840903413565

    2010-01-01

    The simulation of blood flow and pressure in arteries requires outflow boundary conditions that incorporate models of downstream domains. We previously described a coupled multidomain method to couple analytical models of the downstream domains with 3D numerical models of the upstream vasculature. This prior work either included pure resistance boundary conditions or impedance boundary conditions based on assumed periodicity of the solution. However, flow and pressure in arteries are not necessarily periodic in time due to heart rate variability, respiration, complex transitional flow or acute physiological changes. We present herein an approach for prescribing lumped parameter outflow boundary conditions that accommodate transient phenomena. We have applied this method to compute haemodynamic quantities in different physiologically relevant cardiovascular models, including patient-specific examples, to study non-periodic flow phenomena often observed in normal subjects and in patients with acquired or congen...

  14. Closure Report for Corrective Action Unit 346: Areas 8, 10 Housekeeping Sites, Nevada Test Site, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    K. B. Campbell

    2003-08-01

    This Closure Report documents the closure activities conducted for Corrective Action Unit (CAU) 346: Areas 8, 10 Housekeeping Sites. CAU 346 is listed in Appendix III of the Federal Facility Agreement and Consent Order (FFACO, 1996) and consists of the following 14 Corrective Action Sites (CASs) located in Areas 8 and 10 of the Nevada Test Site (NTS): (1) CAS 08-22-04: Drums (2); (2) CAS 08-22-11: Drums; Bucket; (3) CAS 08-24-02: Battery; (4) CAS 10-14-01: Transformer; (5) CAS 10-22-06: Drum (Gas Block); (6) CAS 10-22-10: Drum (Gas Block); (7) CAS 10-22-12: Drum (Gas Block); (8) CAS 10-22-13: Drum (Gas Block); (9) CAS 10-22-16: Drum (Gas Block); (10) CAS 10-22-22: Drum; (11) CAS 10-22-25: Drum; (12) CAS 10-22-36: Paint Can; (13) CAS 10-22-37: Gas Block; and (14) CAS 10-24-11: Battery. Closure activities consisted of closing each CAS by removing debris and/or material, disposing of the generated waste, and verifying that each site was clean-closed by visual inspection and/or laboratory analysis of soil verification samples.

  15. Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania

    Directory of Open Access Journals (Sweden)

    Celso Cruz Tavares

    1992-12-01

    Full Text Available The molecular karyotypes for 20 reference strais of species complexes of Leishmania were determined by contour-clamped homogeneous eletric field (CHEF electrosphoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of house-keeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions of optimal separation of chromosomes, wich resolved DNA molecules as large as 2,500 kilobase pairs (kb. Chromosomes were polymorphic in number (22-30 and size (200-2,500 kb of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, wich allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potencial of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.

  16. Poly(C)-binding protein 1 (PCBP1) mediates housekeeping degradation of mitochondrial antiviral signaling (MAVS)

    Institute of Scientific and Technical Information of China (English)

    Xiang Zhou; Fuping You; Huihui Chen; Zhengfan Jiang

    2012-01-01

    Mitochondrial antiviral signaling (MAVS) is a key adaptor in cellular antiviral innate immunity.We previously identified poly(C)-binding protein 2 (PCBP2) as a feedback inhibitor of MAVS that facilitates its degradation after viral infection,but little is known about the regulatory potential of poly(C)-binding protein 1 (PCBP1),which highly resembles PCBP2.Here we report that PCBP1 mediates housekeeping degradation of MAVS using the same mechanism as PCBP2 employs.Overexpression of PCBP1 impairs MAVS-mediated antiviral responses,while knockdown of PCBP1 exerts the opposite effect.The suppression is due to PCBP1-induced MAVS degradation.We observe that PCBP1 and PCBP2 show synergy in MAVS inhibition,but their expression patterns are distinct:PCBP1 is stably and abundantly expressed,while PCBP2 shows low basal expression with rapid induction after infection.Individual knockdown and subcellular fractionation analyses reveal that unlike the postinfection inhibitor PCBP2,PCBP1 continuously eliminates cellular MAVS.Our findings unravel a critical role of PCBP1 in regulating MAVS for both finetuning the antivirai immunity and preventing inflammation.

  17. Identification of housekeeping genes suitable for gene expression analysis in Jian carp (Cyprinus carpio var. jian).

    Science.gov (United States)

    Tang, Yong-kai; Yu, Ju-hua; Xu, Pao; Li, Jian-lin; Li, Hong-xia; Ren, Hong-tao

    2012-10-01

    Jian carp (Cyprinus carpio var. jian) is an important economic fish species cultured in China. In this report, we performed a systematic analysis to identify an appropriate housekeeping (HK) gene for the study of gene expression in Jian carp. For this purpose, partial DNA sequences of four potential candidate genes (elongation factor 1 alpha (EF-1α), glyceraldehyde-3-phosphate (GAPDH), beta-actin (ACTB), and 18S ribosomal RNA (18S rRNA) were isolated, and their expression levels were studied using RNA extracted from nine tissues (forebrain, hypothalamus, liver, fore-intestine, hind-intestine, ovary, muscle, heart, kidney) in juvenile and adult Jian carp. Gene expression levels were quantified by quantitative real time RT-PCR (qRT-PCR), and expression stability was evaluated by comparing the coefficients of variation (CV) of the Ct values. The results showed that EF-1α was the most suitable HK gene in all tissues of juvenile and adult Jian carp. However, at distinct juvenile and adult developmental stages, there was not a single optimal gene for normalization of expression levels in all tissues. EF-1α was the most stable gene only in forebrain, hypothalamus, liver, heart, and kidney. These results provide data that can be expected to aid gene expression analysis in Jian carp research, but underline the importance of identifying the optimal HK gene for each new experimental paradigm.

  18. Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia

    Directory of Open Access Journals (Sweden)

    Guilherme Silva Julian

    Full Text Available ABSTRACT Obstructive sleep apnea (OSA has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2−ΔCt (threshold cycle data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised.

  19. Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases.

    Science.gov (United States)

    Gutierrez, Belen; Escudero, Jose A; San Millan, Alvaro; Hidalgo, Laura; Carrilero, Laura; Ovejero, Cristina M; Santos-Lopez, Alfonso; Thomas-Lopez, Daniel; Gonzalez-Zorn, Bruno

    2012-05-01

    Arm/Rmt methyltransferases have emerged recently in pathogenic bacteria as enzymes that confer high-level resistance to 4,6-disubstituted aminoglycosides through methylation of the G1405 residue in the 16S rRNA (like ArmA and RmtA to -E). In prokaryotes, nucleotide methylations are the most common type of rRNA modification, and they are introduced posttranscriptionally by a variety of site-specific housekeeping enzymes to optimize ribosomal function. Here we show that while the aminoglycoside resistance methyltransferase RmtC methylates G1405, it impedes methylation of the housekeeping methyltransferase RsmF at position C1407, a nucleotide that, like G1405, forms part of the aminoglycoside binding pocket of the 16S rRNA. To understand the origin and consequences of this phenomenon, we constructed a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF(+), ΔrsmF, rsmF(+) rmtC(+), and ΔrsmF rmtC(+). When analyzed for the antimicrobial resistance pattern, the ΔrsmF bacteria had a decreased susceptibility to aminoglycosides, including 4,6- and 4,5-deoxystreptamine aminoglycosides, showing that the housekeeping methylation at C1407 is involved in intrinsic aminoglycoside susceptibility in E. coli. Competition experiments between the isogenic E. coli strains showed that, contrary to expectation, acquisition of rmtC does not entail a fitness cost for the bacterium. Finally, matrix-assisted laser desorption ionization (MALDI) mass spectrometry allowed us to determine that RmtC methylates the G1405 residue not only in presence but also in the absence of aminoglycoside antibiotics. Thus, the coupling between housekeeping and acquired methyltransferases subverts the methylation architecture of the 16S rRNA but elicits Arm/Rmt methyltransferases to be selected and retained, posing an important threat to the usefulness of aminoglycosides worldwide.

  20. A comparative study of the bactericidal activity and daily disinfection housekeeping surfaces by a new portable pulsed UV radiation device.

    Science.gov (United States)

    Umezawa, Kazuo; Asai, Satomi; Inokuchi, Sadaki; Miyachi, Hayato

    2012-06-01

    Daily cleaning and disinfecting of non-critical surfaces in the patient-care areas are known to reduce the occurrence of health care-associated infections. However, the conventional means for decontamination of housekeeping surfaces of sites of frequent hand contact such as manual disinfection using ethanol wipes are laborious and time-consuming in daily practice. This study evaluated a newly developed portable pulsed ultraviolet (UV) radiation device for its bactericidal activity in comparison with continuous UV-C, and investigated its effect on the labor burden when implemented in a hospital ward. Pseudomonas aeruginosa, Multidrug-resistant P. aeruginosa, Escherichia coli, Acinetobacter baumannii, Amikacin and Ciprofloxacin-resistant A. baumannii, Staphylococcus aureus, Methicillin-resistant S. aureus and Bacillus cereus were irradiated with pulsed UV or continuous UV-C. Pulsed UV and continuous UV-C required 5 and 30 s of irradiation, respectively, to attain bactericidal activity with more than 2Log growth inhibition of all the species. The use of pulsed UV in daily disinfection of housekeeping surfaces reduced the working hours by half in comparison to manual disinfection using ethanol wipes. The new portable pulsed UV radiation device was proven to have a bactericidal activity against critical nosocomial bacteria, including antimicrobial-resistant bacteria after short irradiation, and was thus found to be practical as a method for disinfecting housekeeping surfaces and decreasing the labor burden.

  1. Regulatory MicroRNA Networks: Complex Patterns of Target Pathways for Disease-related and Housekeeping MicroRNAs

    Directory of Open Access Journals (Sweden)

    Sachli Zafari

    2015-06-01

    Full Text Available Blood-based microRNA (miRNA signatures as biomarkers have been reported for various pathologies, including cancer, neurological disorders, cardiovascular diseases, and also infections. The regulatory mechanism behind respective miRNA patterns is only partially understood. Moreover, “preserved” miRNAs, i.e., miRNAs that are not dysregulated in any disease, and their biological impact have been explored to a very limited extent. We set out to systematically determine their role in regulatory networks by defining groups of highly-dysregulated miRNAs that contribute to a disease signature as opposed to preserved housekeeping miRNAs. We further determined preferential targets and pathways of both dysregulated and preserved miRNAs by computing multi-layer networks, which were compared between housekeeping and dysregulated miRNAs. Of 848 miRNAs examined across 1049 blood samples, 8 potential housekeepers showed very limited expression variations, while 20 miRNAs showed highly-dysregulated expression throughout the investigated blood samples. Our approach provides important insights into miRNAs and their role in regulatory networks. The methodology can be applied to systematically investigate the differences in target genes and pathways of arbitrary miRNA sets.

  2. Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus.

    Science.gov (United States)

    Hibbeler, Sascha; Scharsack, Joern P; Becker, Sven

    2008-01-29

    During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research. In this context, the expression of immune response genes in teleost fish has been surveyed for scientific and commercial purposes. Despite the fact that it was shown in teleostei and other taxa that the gene for beta-actin is not the most stably expressed housekeeping gene (HKG), depending on the tissue and experimental treatment, the gene has been used as a reference gene in such studies. In the three-spined stickleback, Gasterosteus aculeatus, other HKG than the one for beta-actin have not been established so far. To establish a reliable method for the measurement of immune gene expression in Gasterosteus aculeatus, sequences from the now available genome database and an EST library of the same species were used to select oligonucleotide primers for HKG, in order to perform quantitative reverse-transcription (RT) PCR. The expression stability of ten candidate reference genes was evaluated in three different tissues, and in five parasite treatment groups, using the three algorithms BestKeeper, geNorm and NormFinder. Our results showed that in most of the tissues and treatments HKG that could not be used so far due to unknown sequences, proved to be more stably expressed than the one for beta-actin. As they were the most stably expressed genes in all tissues examined, we suggest using the genes for the L13a ribosomal binding protein and ubiquitin as alternative or additional reference genes in expression analysis in Gasterosteus aculeatus.

  3. Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus

    Directory of Open Access Journals (Sweden)

    Becker Sven

    2008-01-01

    Full Text Available Abstract Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research. In this context, the expression of immune response genes in teleost fish has been surveyed for scientific and commercial purposes. Despite the fact that it was shown in teleostei and other taxa that the gene for beta-actin is not the most stably expressed housekeeping gene (HKG, depending on the tissue and experimental treatment, the gene has been used as a reference gene in such studies. In the three-spined stickleback, Gasterosteus aculeatus, other HKG than the one for beta-actin have not been established so far. Results To establish a reliable method for the measurement of immune gene expression in Gasterosteus aculeatus, sequences from the now available genome database and an EST library of the same species were used to select oligonucleotide primers for HKG, in order to perform quantitative reverse-transcription (RT PCR. The expression stability of ten candidate reference genes was evaluated in three different tissues, and in five parasite treatment groups, using the three algorithms BestKeeper, geNorm and NormFinder. Our results showed that in most of the tissues and treatments HKG that could not be used so far due to unknown sequences, proved to be more stably expressed than the one for beta-actin. Conclusion As they were the most stably expressed genes in all tissues examined, we suggest using the genes for the L13a ribosomal binding protein and ubiquitin as alternative or additional reference genes in expression analysis in Gasterosteus aculeatus.

  4. An integrated approach for non-periodic dynamic response prediction of complex structures: Numerical and experimental analysis

    Science.gov (United States)

    Rahneshin, Vahid; Chierichetti, Maria

    2016-09-01

    In this paper, a combined numerical and experimental method, called Extended Load Confluence Algorithm, is presented to accurately predict the dynamic response of non-periodic structures when little or no information about the applied loads is available. This approach, which falls into the category of Shape Sensing methods, inputs limited experimental information acquired from sensors to a mapping algorithm that predicts the response at unmeasured locations. The proposed algorithm consists of three major cores: an experimental core for data acquisition, a numerical core based on Finite Element Method for modeling the structure, and a mapping algorithm that improves the numerical model based on a modal approach in the frequency domain. The robustness and precision of the proposed algorithm are verified through numerical and experimental examples. The results of this paper demonstrate that without a precise knowledge of the loads acting on the structure, the dynamic behavior of the system can be predicted in an effective and precise manner after just a few iterations.

  5. Control of morphological defects at the boundary between the periodic and non-periodic patterns in directed self-assembly process

    Science.gov (United States)

    Yoshida, Akihisa; Yoshimoto, Kenji; Ohshima, Masahiro; Kodera, Katsuyoshi; Naka, Yoshihiro; Kanai, Hideki; Kobayashi, Sachiko; Maeda, Simon; Jiravanichsakul, Phubes; Kobayashi, Katsutoshi; Aoyama, Hisako

    2016-04-01

    In this study, we investigated a directed self-assembly (DSA) flow that could include a non-periodic pattern (i.e., wide line) lying in between the periodic line/space patterns, in a relatively simple and inexpensive way. A symmetric poly(styrene-block-methyl methacrylate) (PS-b-PMMA) with the natural periodicity (L0) of 30 nm was employed here. Our DSA flow has two key features. First, we used a hybrid approach that combined chemoepitaxy and graphoepitaxy methods to generate PMMA-attractive pinning guide patterns directly from ArF resist. Second, we attempted to utilize both the perpendicular lamellae in the periodic regions and the horizontal lamellae on the non-periodic pattern as an etch template. The advantage of this process will be a reduction of the number of lithographic processes, whereas the challenge is how to control the mixed morphologies at the boundary between the periodic and non-periodic regions. Our preliminary results from simulations and experiments showed that, in order to generate the horizontal lamellae on the non-periodic pattern, the PS-b-PMMA thickness on top of the non-periodic guide pattern should roughly match to ~1 L0, and the width of the non-periodic pattern should be larger than ~3-4 L0. In addition, the space between the periodic and non-periodic regions was found to be critical and it should be basically equal to the space between the guiding pins in the periodic regions (~75 nm) to minimize the formation of fingerprint morphology at the boundaries.

  6. Prevalence and factors associated with minor psychiatric disorders in hospital housekeeping workers.

    Science.gov (United States)

    Marconato, Cintia da Silva; Magnago, Ana Carolina de Souza; Magnago, Tânia Solange Bosi de Souza; Dalmolin, Graziele de Lima; Andolhe, Rafaela; Tavares, Juliana Petri

    2017-06-12

    Investigating the prevalence and factors associated with minor psychiatric disorders (MPDs) in Hospital housekeeping workers. A cross-sectional study carried out in 2013 with workers from the cleaning service of a public university hospital in Rio Grande do Sul, Brazil. Data were collected through a form containing sociodemographic, occupational, habits and health variables. The Self-Reporting Questionnaire-20 was used in order to evaluate MPDs. The study population consisted of 161 workers. The overall prevalence of suspected MPD was 29.3%. The chances of suspected MPDs were higher in workers with Effort-Reward Imbalance, those who did not have time or who occasionally had time for leisure activities, and those taking medications. The prevalence of MPDs was similar to that found in the literature for health workers. Therefore, we consider it important to include these workers in institutional programs for continuing health education. Investigar a prevalência e os fatores associados aos Distúrbios Psíquicos Menores (DPMs) em trabalhadores do Serviço Hospitalar de Limpeza. Estudo transversal, realizado em 2013, com trabalhadores do serviço de limpeza de um hospital universitário público do Rio Grande do Sul, Brasil. Os dados foram coletados por meio de um formulário contendo variáveis sociodemográficas, laborais, hábitos e saúde. Para avaliação dos DPMs utilizou-se do Self-Reporting Questionnaire-20. A população do estudo foi composta pelos 161 trabalhadores. A prevalência global para suspeição de DPM foi de 29,3%. As chances de suspeição de DPMs foram maiores nos trabalhadores em Desequilíbrio Esforço-Recompensa, nos que não tinham ou às vezes tinham tempo para o lazer e naqueles que faziam uso de medicação. A prevalência de DPMs assemelhou-se à encontrada na literatura em trabalhadores da área saúde. Portanto, considera-se importante a inclusão desses trabalhadores em programas institucionais de educação permanente em saúde.

  7. How to make stripes: deciphering the transition from non-periodic to periodic patterns in Drosophila segmentation.

    Science.gov (United States)

    Schroeder, Mark D; Greer, Christina; Gaul, Ulrike

    2011-07-01

    The generation of metameric body plans is a key process in development. In Drosophila segmentation, periodicity is established rapidly through the complex transcriptional regulation of the pair-rule genes. The 'primary' pair-rule genes generate their 7-stripe expression through stripe-specific cis-regulatory elements controlled by the preceding non-periodic maternal and gap gene patterns, whereas 'secondary' pair-rule genes are thought to rely on 7-stripe elements that read off the already periodic primary pair-rule patterns. Using a combination of computational and experimental approaches, we have conducted a comprehensive systems-level examination of the regulatory architecture underlying pair-rule stripe formation. We find that runt (run), fushi tarazu (ftz) and odd skipped (odd) establish most of their pattern through stripe-specific elements, arguing for a reclassification of ftz and odd as primary pair-rule genes. In the case of run, we observe long-range cis-regulation across multiple intervening genes. The 7-stripe elements of run, ftz and odd are active concurrently with the stripe-specific elements, indicating that maternal/gap-mediated control and pair-rule gene cross-regulation are closely integrated. Stripe-specific elements fall into three distinct classes based on their principal repressive gap factor input; stripe positions along the gap gradients correlate with the strength of predicted input. The prevalence of cis-elements that generate two stripes and their genomic organization suggest that single-stripe elements arose by splitting and subfunctionalization of ancestral dual-stripe elements. Overall, our study provides a greatly improved understanding of how periodic patterns are established in the Drosophila embryo.

  8. Assessment of House-Keeping Practices of Generators Used As Alternative Source of Power in Residential Buildings

    Directory of Open Access Journals (Sweden)

    Wahab, A.B.

    2016-08-01

    Full Text Available Inadequate power supply from the national grid over the years has led to the ubiquitous use of various types of generating sets by the occupants of buildings. This study was carried out to identify and examine house-keeping practices adopted by occupants of residential building in Ibadan Metropolis, Nigeria before and during use of generating sets. The study area was stratified into core, transition and suburban residential zones. Data were obtained by carrying out field observations and administering questionnaires on the occupants of residential buildings. Descriptive and inferential statistical techniques were used to analyse the data collected. The findings revealed that the most adopted house-keeping practice by residential buildings occupants in the core and transition zone was provision of balanced rest position (HKPI = 0.6425, 0.7353, and in the suburban zone, it was putting the generator in a ventilated environment (HKPI = 0.8246. The mean distance of positioning generators from external walls of buildings in the core, transition and suburban residential zones were 2.09, 3.59 and 7.39m respectively. The variation in the mode and level of house-keeping practices adopted was significantly influenced by the socio-economic characteristics of the respondents, typology of buildings and their degree of compliance with statutory environmental and planning laws. The study recommended that in the face of poor power supply in the country, building occupants should beeducated and enforced to position their generating sets in well-designed outdoor enclosure features and at specified distance limits from external walls of their buildings.

  9. Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray

    Directory of Open Access Journals (Sweden)

    Niggli Felix K

    2006-06-01

    Full Text Available Abstract Background The Epstein-Barr virus (EBV is associated with lymphoid malignancies, including Burkitt's lymphoma (BL, and can transform human B cells in vitro. EBV-harboring cell lines are widely used to investigate lymphocyte transformation and oncogenesis. Qualitative EBV gene expression has been extensively described, but knowledge of quantitative transcription is lacking. We hypothesized that transcription levels of EBNA1, the gene essential for EBV persistence within an infected cell, are similar in BL cell lines. Results To compare quantitative gene transcription in the BL cell lines Namalwa, Raji, Akata, Jijoye, and P3HR1, we developed an oligonucleotide microarray chip, including 17 housekeeping genes, six latent EBV genes (EBNA1, EBNA2, EBNA3A, EBNA3C, LMP1, LMP2, and four lytic EBV genes (BZLF1, BXLF2, BKRF2, BZLF2, and used the cell line B95.8 as a reference for EBV gene transcription. Quantitative polymerase chain reaction assays were used to validate microarray results. We found that transcription levels of housekeeping genes differed considerably among BL cell lines. Using a selection of housekeeping genes with similar quantitative transcription in the tested cell lines to normalize EBV gene transcription data, we showed that transcription levels of EBNA1 were quite similar in very different BL cell lines, in contrast to transcription levels of other EBV genes. As demonstrated with Akata cells, the chip allowed us to accurately measure EBV gene transcription changes triggered by treatment interventions. Conclusion Our results suggest uniform EBNA1 transcription levels in BL and that microarray profiling can reveal novel insights on quantitative EBV gene transcription and its impact on lymphocyte biology.

  10. ACONC Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — ACONC files containing simulated ozone and PM2.5 fields that were used to create the model difference plots shown in the journal article. This dataset is associated...

  11. 831 Files

    Data.gov (United States)

    Social Security Administration — SSA-831 file is a collection of initial and reconsideration adjudicative level DDS disability determinations. (A few hearing level cases are also present, but the...

  12. Closure Report for Housekeeping Category Corrective Action Unit 387: Spill Sites and Releases, Nevada Test Site, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    T. M. Fitzmaurice

    2001-10-01

    This Closure Report documents the closure activities conducted for CAU 387: Spill Sites and Releases. Closure activities were performed in two phases. Phase I activities consisted of collecting waste characterization samples of soil at appropriate sites. The results were used to determine how waste generated during closure activities would be handled and disposed (i.e., as nonhazardous sanitary or hazardous waste). Phase 2 activities consisted of closing each CAS by removing debris and/or soil, disposing of the generated waste, and verifying that each site was clean-closed by visual inspection and/or collecting soil verification samples for laboratory analysis. Additionally, seven sites were closed with no further action after concurrence with Nevada Division of Environmental Protection (NDEP). Four other sites were moved into different CAUs in Appendix III of the FFACO because the housekeeping process was not adequate to close them. Copies of the analytical results for the site verification samples are included in Appendix A. Copies of the Sectored Housekeeping Site Closure Verification Forms for each of the 16 CAS are included in Appendix B.

  13. Identification of housekeeping genes suitable for gene expression analysis in the pearl mussel, Hyriopsis cumingii, during biomineralization.

    Science.gov (United States)

    Bai, Zhiyi; Lin, Jingyun; Ma, Keyi; Wang, Guiling; Niu, Donghong; Li, Jiale

    2014-08-01

    Quantitative real-time polymerase chain reaction is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcript levels across different samples and tissues. The freshwater pearl, Hyriopsis cumingii (Lea), is an important economic species cultured in China. To date, no reference genes for gene expression analysis in this species have been validated. This study aimed to compare the relative expression of seven housekeeping genes across different tissue types and in the mantle or pearl sac during three biomineralization processes: seasonal shell growth, shell healing and pearl-sac formation in H. cumingii. Three programs evaluated the expression stabilities of the seven genes: BestKeeper, geNorm and NormFinder. The beta actin gene (ACTB), commonly used as a housekeeping gene in many studies, was the least stable. The expressions of Ubiquitin (Ubi) and Ribosomal protein L18 (Rpl18) and Elongation factor 1-alpha (EF1α) were more stable than the remaining four genes. Therefore, we suggest that Ubi, Rpl18 and EF1α are suitable reference genes. The three selected reference genes are expected to facilitate analysis of gene expressions during shell or pearl formation in H. cumingii.

  14. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.

    Science.gov (United States)

    Nicot, Nathalie; Hausman, Jean-François; Hoffmann, Lucien; Evers, Danièle

    2005-11-01

    Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is referred to one or several internal control genes, which should not fluctuate during treatments. Here, the non-regulation of seven housekeeping genes (beta-tubulin, cyclophilin, actin, elongation factor 1-alpha (ef1alpha), 18S rRNA, adenine phosphoribosyl transferase (aprt), and cytoplasmic ribosomal protein L2) during biotic (late blight) and abiotic stresses (cold and salt stress) was tested on potato plants using geNorm software. Results from the three experimental conditions indicated that ef1alpha was the most stable among the seven tested. The expression of the other housekeeping genes tested varied upon stress. In parallel, a study of the variability of expression of hsp20.2, shown to be implicated in late blight stress, was realized. The relative quantification of the hsp20.2 gene varied according to the internal control and the number of internal controls used, thus highlighting the importance of the choice of internal controls in such experiments.

  15. Identifiable Data Files - Denominator File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Denominator File combines Medicare beneficiary entitlement status information from administrative enrollment records with third-party payer information and GHP...

  16. Computer files.

    Science.gov (United States)

    Malik, M

    1995-02-01

    From what has been said, several recommendations can be made for users of small personal computers regardless of which operating system they use. If your computer has a large hard disk not specially required by any single application, organize the disk into a small number of volumes. You will then be using the computer as if it had several smaller disks, which will help you to create a logical file structure. The size of individual volumes has to be selected carefully with respect to the files kept in each volume. Otherwise, it may be that you will have too much space in one volume and not enough in another. In each volume, organize the structure of directories and subdirectories logically so that they correspond to the logic of your file content. Be aware of the fact that the directories suggested as default when installing new software are often not the optimum. For instance, it is better to put different graphics packages under a common subdirectory rather than to install them at the same level as all other packages including statistics, text processors, etc. Create a special directory for each task you use the computer. Note that it is a bad practice to keep many different and logically unsorted files in the root directory of any of your volumes. Only system and important service files should be kept there. Although any file may be written all over the disk, access to it will be faster if it is written over the minimum number of cylinders. From time to time, use special programs that reorganize your files in this way.(ABSTRACT TRUNCATED AT 250 WORDS)

  17. Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states

    Directory of Open Access Journals (Sweden)

    Osailan Samira

    2008-07-01

    Full Text Available Abstract Background Real-time PCR is a reliable tool with which to measure mRNA transcripts, and provides valuable information on gene expression profiles. Endogenous controls such as housekeeping genes are used to normalise mRNA levels between samples for sensitive comparisons of mRNA transcription. Selection of the most stable control gene(s is therefore critical for the reliable interpretation of gene expression data. For the purpose of this study, 7 commonly used housekeeping genes were investigated in salivary submandibular glands under normal, inflamed, atrophic and regenerative states. Results The program NormFinder identified the suitability of HPRT to use as a single gene for normalisation within the normal, inflamed and regenerative states, and GAPDH in the atrophic state. For normalisation to multiple housekeeping genes, for each individual state, the optimal number of housekeeping genes as given by geNorm was: ACTB/UBC in the normal, ACTB/YWHAZ in the inflamed, ACTB/HPRT in the atrophic and ACTB/GAPDH in the regenerative state. The most stable housekeeping gene identified between states (compared to normal was UBC. However, ACTB, identified as one of the most stably expressed genes within states, was found to be one of the most variable between states. Furthermore we demonstrated that normalising between states to ACTB, rather than UBC, introduced an approximately 3 fold magnitude of error. Conclusion Using NormFinder, our studies demonstrated the suitability of HPRT to use as a single gene for normalisation within the normal, inflamed and regenerative groups and GAPDH in the atrophic group. However, if normalising to multiple housekeeping genes, we recommend normalising to those identified by geNorm. For normalisation across the physiological states, we recommend the use of UBC.

  18. Topological and organizational properties of the products of house-keeping and tissue-specific genes in protein-protein interaction networks.

    Science.gov (United States)

    Lin, Wen-Hsien; Liu, Wei-Chung; Hwang, Ming-Jing

    2009-03-11

    Human cells of various tissue types differ greatly in morphology despite having the same set of genetic information. Some genes are expressed in all cell types to perform house-keeping functions, while some are selectively expressed to perform tissue-specific functions. In this study, we wished to elucidate how proteins encoded by human house-keeping genes and tissue-specific genes are organized in human protein-protein interaction networks. We constructed protein-protein interaction networks for different tissue types using two gene expression datasets and one protein-protein interaction database. We then calculated three network indices of topological importance, the degree, closeness, and betweenness centralities, to measure the network position of proteins encoded by house-keeping and tissue-specific genes, and quantified their local connectivity structure. Compared to a random selection of proteins, house-keeping gene-encoded proteins tended to have a greater number of directly interacting neighbors and occupy network positions in several shortest paths of interaction between protein pairs, whereas tissue-specific gene-encoded proteins did not. In addition, house-keeping gene-encoded proteins tended to connect with other house-keeping gene-encoded proteins in all tissue types, whereas tissue-specific gene-encoded proteins also tended to connect with other tissue-specific gene-encoded proteins, but only in approximately half of the tissue types examined. Our analysis showed that house-keeping gene-encoded proteins tend to occupy important network positions, while those encoded by tissue-specific genes do not. The biological implications of our findings were discussed and we proposed a hypothesis regarding how cells organize their protein tools in protein-protein interaction networks. Our results led us to speculate that house-keeping gene-encoded proteins might form a core in human protein-protein interaction networks, while clusters of tissue-specific gene

  19. Molecular Phylogenetics and Temporal Diversification in the Genus Aeromonas Based on the Sequences of Five Housekeeping Genes

    Science.gov (United States)

    Lorén, J. Gaspar; Farfán, Maribel; Fusté, M. Carmen

    2014-01-01

    Several approaches have been developed to estimate both the relative and absolute rates of speciation and extinction within clades based on molecular phylogenetic reconstructions of evolutionary relationships, according to an underlying model of diversification. However, the macroevolutionary models established for eukaryotes have scarcely been used with prokaryotes. We have investigated the rate and pattern of cladogenesis in the genus Aeromonas (γ-Proteobacteria, Proteobacteria, Bacteria) using the sequences of five housekeeping genes and an uncorrelated relaxed-clock approach. To our knowledge, until now this analysis has never been applied to all the species described in a bacterial genus and thus opens up the possibility of establishing models of speciation from sequence data commonly used in phylogenetic studies of prokaryotes. Our results suggest that the genus Aeromonas began to diverge between 248 and 266 million years ago, exhibiting a constant divergence rate through the Phanerozoic, which could be described as a pure birth process. PMID:24586399

  20. Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia.

    Science.gov (United States)

    Julian, Guilherme Silva; Oliveira, Renato Watanabe de; Tufik, Sergio; Chagas, Jair Ribeiro

    2016-01-01

    Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA) on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2-ΔCt (threshold cycle) data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised. RESUMO A apneia obstrutiva do sono (AOS) tem sido associada ao estresse oxidativo e a várias consequências cardiovasculares, tais como risco aumentado de doença cardiovascular. A PCR quantitativa em tempo real é frequentemente empregada para avaliar alterações na expressão gênica em modelos experimentais. Neste estudo, analisamos os efeitos da hipóxia intermitente crônica (um modelo experimental de AOS) na expressão de genes de referência no ventrículo cardíaco esquerdo de ratos. Análises a partir de quatro abordagens - uso dos algoritmos geNorm, BestKeeper e NormFinder e análise de dados 2-ΔCt (ciclo limiar) - produziram resultados semelhantes: todos os genes mostraram-se adequados para uso, sendo que gliceraldeído-3-fosfato desidrogenase e 18S foram classificados como o mais e o menos estável, respectivamente. A utilização de mais de um gene de referência é altamente recomendada.

  1. Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage.

    Science.gov (United States)

    Quiroz, Felipe Garcia; Posada, Olga M; Gallego-Perez, Daniel; Higuita-Castro, Natalia; Sarassa, Carlos; Hansford, Derek J; Agudelo-Florez, Piedad; López, Luis E

    2010-04-01

    Real-time reverse transcription PCR (RT-qPCR) relies on a housekeeping or normalizer gene whose expression remains constant throughout the experiment. RT-qPCR is commonly used for characterization of human bone marrow mesenchymal stem cells (hBMSCs). However, to the best of our knowledge, there are no studies validating the expression stability of the genes used as normalizers during hBMSCs differentiation. This work aimed to study the stability of the housekeeping genes beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and ribosomal protein L13A (RPL13A) during the osteogenic differentiation of hBMSCs. Their stability was evaluated via RT-qPCR in 14 and 20 day differentiation assays to the osteogenic lineage. Different normalization strategies were evaluated to quantify the osteogenic markers collagen type I, bone sialoprotein and osteonectin. Cell differentiation was confirmed via alizarin red staining. The results demonstrated up-regulation of beta-actin with maximum fold changes (MFC) of 4.38. GAPDH and RPL13A were not regulated by osteogenic media after 14 days and presented average fold changes lower than 2 in 20 day cultures. RPL13A (MFC < 2) had a greater stability when normalizing as a function of culture time compared with GAPDH (MFC

  2. Effect of experimental treatment on GAPDH mRNA expression as a housekeeping gene in human diploid fibroblasts

    Directory of Open Access Journals (Sweden)

    Zainuddin Azalina

    2010-08-01

    Full Text Available Abstract Background Several genes have been used as housekeeping genes and choosing an appropriate reference gene is important for accurate quantitative RNA expression in real time RT-PCR technique. The expression levels of reference genes should remain constant between the cells of different tissues and under different experimental conditions. The purpose of this study was to determine the effect of different experimental treatments on the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH mRNA so that the reliability of GAPDH as reference gene for quantitative real time RT-PCR in human diploid fibroblasts (HDFs can be validated. HDFs in 4 different treatment groups viz; young (passage 4, senescent (passage 30, H2O2-induced oxidative stress and γ-tocotrienol (GTT-treated groups were harvested for total RNA extraction. Total RNA concentration and purity were determined prior to GAPDH mRNA quantification. Standard curve of GAPDH expression in serial diluted total RNA, melting curve analysis and agarose gel electrophoresis were used to determine the reliability of GAPDH as reference gene. Results HDFs with different experimental treatments exhibited diverse cell morphology with different expression of senescence-associated β-galactosidase (SA β-gal activity. However the expression level of GAPDH was consistent in all treatment groups. Conclusion The study demonstrated that GAPDH is reliable as reference gene for quantitative gene expression analysis in HDFs. Therefore it can be used as housekeeping gene for quantitative real time RT-PCR technique in human diploid fibroblasts particularly in studying cellular senescence.

  3. Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection.

    Science.gov (United States)

    Palau, Montserrat; Kulmann, Marcos; Ramírez-Lázaro, María José; Lario, Sergio; Quilez, María Elisa; Campo, Rafael; Piqué, Núria; Calvet, Xavier; Miñana-Galbis, David

    2016-12-01

    Helicobacter pylori infects human stomachs of over half the world's population, evades the immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal and gastric ulcers, MALT lymphoma and progression to gastric cancer could be developed. Several virulence factors such as flagella, lipopolysaccharide, adhesins and especially the vacuolating cytotoxin VacA and the oncoprotein CagA have been described for H. pylori. Despite the extensive published data on H. pylori, more research is needed to determine new virulence markers, the exact mode of transmission or the role of multiple infection. Amplification and sequencing of six housekeeping genes (amiA, cgt, cpn60, cpn70, dnaJ, and luxS) related to H. pylori pathogenesis have been performed in order to evaluate their usefulness for the specific detection of H. pylori, the genetic discrimination at strain level and the detection of multiple infection. A total of 52 H. pylori clones, isolated from 14 gastric biopsies from 11 patients, were analyzed for this purpose. All genes were specifically amplified for H. pylori and all clones isolated from different patients were discriminated, with gene distances ranged from 0.9 to 7.8%. Although most clones isolated from the same patient showed identical gene sequences, an event of multiple infection was detected in all the genes and microevolution events were showed for amiA and cpn60 genes. These results suggested that housekeeping genes could be useful for H. pylori detection and to elucidate the mode of transmission and the relevance of the multiple infection. © 2016 John Wiley & Sons Ltd.

  4. Common File Formats.

    Science.gov (United States)

    Mills, Lauren

    2014-03-21

    An overview of the many file formats commonly used in bioinformatics and genome sequence analysis is presented, including various data file formats, alignment file formats, and annotation file formats. Example workflows illustrate how some of the different file types are typically used.

  5. HUD GIS Boundary Files

    Data.gov (United States)

    Department of Housing and Urban Development — The HUD GIS Boundary Files are intended to supplement boundary files available from the U.S. Census Bureau. The files are for community planners interested in...

  6. Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).

    Science.gov (United States)

    Song, Hao; Dang, Xin; He, Yuan-Qiu; Zhang, Tao; Wang, Hai-Yan

    2017-01-01

    The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of development. However, to date no studies have validated housekeeping genes in R. venosa for use as internal controls for qRT-PCR. In this study, we selected the following 13 candidate genes for suitability as internal controls: elongation factor-1α (EF-1α), α-actin (ACT), cytochrome c oxidase subunit 1 (COX1), nicotinamide adenine dinucleotide dehydrogenase (ubiquinone) 1α subcomplex subunit 7 (NDUFA7), 60S ribosomal protein L5 (RL5), 60S ribosomal protein L28 (RL28), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-tubulin (TUBB), 40S ribosomal protein S25 (RS25), 40S ribosomal protein S8 (RS8), ubiquitin-conjugating enzyme E2 (UBE2), histone H3 (HH3), and peptidyl-prolyl cis-trans isomerase A (PPIA). We measured the expression levels of these 13 candidate internal controls in eight different tissues and twelve larvae developmental stages by qRT-PCR. Further analysis of the expression stability of the tested genes was performed using GeNorm and RefFinder algorithms. Of the 13 candidate genes tested, we found that EF-1α was the most stable internal control gene in almost all adult tissue samples investigated with RL5 and RL28 as secondary choices. For the normalization of a single specific tissue, we suggested that EF-1α and NDUFA7 are the best combination in gonad, as well as COX1 and RL28 for intestine, EF-1α and RL5 for kidney, EF-1α and COX1 for gill, EF-1α and RL28 for Leiblein and mantle, EF-1α, RL5, and NDUFA7 for liver, GAPDH, PPIA, and RL28 for hemocyte. From a developmental perspective, we found that RL28 was the most stable gene in all developmental stages measured

  7. Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

    Directory of Open Access Journals (Sweden)

    Looft Christian

    2011-10-01

    Full Text Available Abstract Background Gene expression analysis using real-time RT-PCR (qRT-PCR is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes or internal control genes is required. The stability of reference genes has a tremendous effect on the results of relative quantification of gene expression by qRT-PCR. The expression stability of reference genes could vary according to tissues, age of individuals and experimental conditions. In the pig however, very little information is available on the expression stability of reference genes. The aim of this research was therefore to develop a new set of reference genes which can be used for normalization of mRNA expression data of genes expressed in varieties of porcine tissues at different ages. Results The mRNA expression stability of nine commonly used reference genes (B2M, BLM, GAPDH, HPRT1, PPIA, RPL4, SDHA, TBP and YWHAZ was determined in varieties of tissues collected from newborn, young and adult pigs. geNorm, NormFinder and BestKeeper software were used to rank the genes according to their stability. geNorm software revealed that RPL4, PPIA and YWHAZ showed high stability in newborn and adult pigs, while B2M, YWHAZ and SDHA showed high stability in young pigs. In all cases, GAPDH showed the least stability in geNorm. NormFinder revealed that TBP was the most stable gene in newborn and young pigs, while PPIA was most stable in adult pigs. Moreover, geNorm software suggested that the geometric mean of three most stable gene would be the suitable combination for accurate normalization of gene expression study. Conclusions Although, there was discrepancy in the ranking order of reference genes obtained by different analysing software methods, the geometric mean of the RPL4, PPIA and YWHAZ seems to be the most appropriate combination of

  8. 43 CFR 4.1352 - Who may file; where to file; when to file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; where to file; when to file... Indian Lands) § 4.1352 Who may file; where to file; when to file. (a) The applicant or operator may file... to file a timely request constitutes a waiver of the opportunity for a hearing before OSM makes...

  9. Fact File

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    @@ Are delegates selected according to predetermined quotas of ethnicity and gender? The delegates who attend the CPC National Congress represent a broad spectrum of Party members.They include leading officials at various levels, and rank and file Party members working at the front line of production and those from more regular walks of life.A large proportion of the delegates are model Party members who have made outstanding contributions in various sectors and undertakings of the economy, science and technology, national defense, politics and law, education, public relations, public health, culture and sports.

  10. Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model

    Science.gov (United States)

    Julian, Guilherme Silva; de Oliveira, Renato Watanabe; Perry, Juliana Cini; Tufik, Sergio; Chagas, Jair Ribeiro

    2014-01-01

    Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses. PMID:25289636

  11. Validation of housekeeping genes in the brains of rats submitted to chronic intermittent hypoxia, a sleep apnea model.

    Science.gov (United States)

    Julian, Guilherme Silva; de Oliveira, Renato Watanabe; Perry, Juliana Cini; Tufik, Sergio; Chagas, Jair Ribeiro

    2014-01-01

    Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses.

  12. Validation of housekeeping genes in the brains of rats submitted to chronic intermittent hypoxia, a sleep apnea model.

    Directory of Open Access Journals (Sweden)

    Guilherme Silva Julian

    Full Text Available Obstructive sleep apnea (OSA is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH, which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses.

  13. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file consists of two data files, one for CLIA labs and one for 18 other provider types. The file names are CLIA and OTHER. If downloading the file, note it...

  14. Validation of housekeeping genes as internal controls for studying the gene expression in Pyropia haitanensis (Bangiales, Rhodophyta) by quantitative real-time PCR

    Institute of Scientific and Technical Information of China (English)

    LI Bing; CHEN Changsheng; XU Yan; JI Dehua; XIE Chaotian

    2014-01-01

    Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these characteristics, gene expression has been investigated at the whole transcriptome level. Gene expres-sion studies using quantitative real-time PCR should start by selecting an appropriate internal control gene;therefore, the absolute expression abundance of six housekeeping genes (18S rRNA (18S), ubiquitin-conju-gating enzyme (UBC), actin (ACT),β-tubulin (TUB), elongation factors 2 (EF2), and glyceraldehyde-3-phos-phate dehydrogenase (GAPDH) examined by the quantitative real-time PCR in samples corresponding to different strains, life-cycle stages and abiotic stress treatments. Their expression stabilities were assessed by the comparative cycle threshold (Ct) method and by two different software packages:geNorm and Norm-Finder. The most stable housekeeping gene is UBC and the least stable housekeeping is GADPH. Thus, it is proposed that the most appropriate internal control gene for expression analyses in P. haitanensis is UBC. The results pave the way for further gene expression analyses of different aspects of P. haitanensis biology including different strains, life-history stages and abiotic stress responses.

  15. Validation of housekeeping genes for the normalization of RT-qPCR expression studies in oral squamous cell carcinoma cell line treated by 5 kinds of chemotherapy drugs.

    Science.gov (United States)

    Song, W; Zhang, W H; Zhang, H; Li, Y; Zhang, Y; Yin, W; Yang, Q

    2016-11-30

    Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become a frequently used strategy in gene expression studies. The relative quantification method is an important and commonly used method for the evaluation of RT-qPCR data. The key of this method is to identify an applicable internal control gene because the usage of different internal control genes may lead to distinct conclusions. Herein, we report the validation of 12 common housekeeping genes for RT-qPCR for gene expression analysis in the Oral squamous cell carcinoma (OSCC) cell line (KB and Tca-8113) treated by 5 kinds of Chemotherapy Drugs. The gene expression stability and applicability of the 12 housekeeping gene candidates were determined using the geNorm, NormFinder, and BestKeeper software programs. Comprehensive analyzing the results of the three software, ALAS1/GAPDH, ALAS1 and GUSB were suggested to be the most stable candidate genes for the study of both KB and Tca-8113 cell line together, KB cell line, and Tca-8113 cell line, respectively. This study provides useful information to normalize gene expression accurately for the investigation of target gene profiling in cell lines of OSCC. Further clarification of tumor molecular expression markers with our recommended housekeeping genes may improve the accuracy of diagnosis and estimation of prognostic factors as well as provide novel personalized treatments for OSCC patients.

  16. The housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT regulates multiple developmental and metabolic pathways of murine embryonic stem cell neuronal differentiation.

    Directory of Open Access Journals (Sweden)

    Tae Hyuk Kang

    Full Text Available The mechanisms by which mutations of the purinergic housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT cause the severe neurodevelopmental Lesch Nyhan Disease (LND are poorly understood. The best recognized neural consequences of HPRT deficiency are defective basal ganglia expression of the neurotransmitter dopamine (DA and aberrant DA neuronal function. We have reported that HPRT deficiency leads to dysregulated expression of multiple DA-related developmental functions and cellular signaling defects in a variety of HPRT-deficient cells, including human induced pluripotent stem (iPS cells. We now describe results of gene expression studies during neuronal differentiation of HPRT-deficient murine ESD3 embryonic stem cells and report that HPRT knockdown causes a marked switch from neuronal to glial gene expression and dysregulates expression of Sox2 and its regulator, genes vital for stem cell pluripotency and for the neuronal/glial cell fate decision. In addition, HPRT deficiency dysregulates many cellular functions controlling cell cycle and proliferation mechanisms, RNA metabolism, DNA replication and repair, replication stress, lysosome function, membrane trafficking, signaling pathway for platelet activation (SPPA multiple neurotransmission systems and sphingolipid, sulfur and glycan metabolism. We propose that the neural aberrations of HPRT deficiency result from combinatorial effects of these multi-system metabolic errors. Since some of these aberrations are also found in forms of Alzheimer's and Huntington's disease, we predict that some of these systems defects play similar neuropathogenic roles in diverse neurodevelopmental and neurodegenerative diseases in common and may therefore provide new experimental opportunities for clarifying pathogenesis and for devising new potential therapeutic targets in developmental and genetic disease.

  17. Use of PMA1 as a housekeeping biomarker for assessment of toxicant-induced stress in Saccharomyces cerevisiae.

    Science.gov (United States)

    Schmitt, Marcel; Schwanewilm, Petra; Ludwig, Jost; Lichtenberg-Fraté, Hella

    2006-02-01

    The brewer's yeast Saccharomyces cerevisiae has emerged as a versatile and robust model system for laboratory use to study toxic effects of various substances. In this study, toxicant-induced stresses of pure compounds were investigated in Saccharomyces cerevisiae utilizing a destabilized version of the green fluorescent protein optimized for expression in yeast (yEGFP3) under control of the promoter of the housekeeping plasma membrane ATPase gene PMA1. The responses of the biomarker upon increasing test compound concentrations were monitored by determining the decrease in fluorescence. The reporter assay deployed a simple and robust protocol for the rapid detection of toxic effects within a 96-well microplate format. Fluorescence emissions were normalized to cell growth determined by absorption and were correlated to internal reference standards. The results were expressed as effective concentrations (EC20). Dose-response experiments were conducted in which yeast cells were exposed in minimal medium and in the presence of 20% fetal calf serum to sublethal concentrations of an array of heavy metals, salt, and a number of stress-inducing compounds (Diclofenac, Lindane, methyl-N-nitro-N-nitrosoguanidine [MNNG], hydroxyurea, and caffeine). Long-term exposure (7 h) played a considerable role in the adaptive response to intoxication compared to early responses at 4 h exposure. The data obtained after 4 h of exposure and expressed as EC20 were compared to 50% inhibitory concentration values derived from cell line and ecotoxicological tests. This study demonstrates the versatility of the novel biomarker to complement existing test batteries to assess contaminant exposure and effects.

  18. Characterization of Rhizobium naphthalenivorans sp. nov. with special emphasis on aromatic compound degradation and multilocus sequence analysis of housekeeping genes.

    Science.gov (United States)

    Kaiya, Shinichi; Rubaba, Owen; Yoshida, Naoko; Yamada, Takeshi; Hiraishi, Akira

    2012-01-01

    Three strains of aerobic chemoorganotrophic naphthalene-degrading bacteria (designated TSY03b(T), TSY04, and TSW01) isolated from sediment of a polychlorinated-dioxin-transforming microcosm were characterized. These strains had Gram-negative-stained, rod-shaped cells measuring 0.6‒0.9 μm in width and 1.2‒3.0 μm in length and were motile by means of peritrichous flagella. Naphthalene was utilized as the sole carbon and energy source, and the transcription of a putative aromatic-ring hydroxylating gene was inducible by naphthalene. The major component of cellular fatty acids was summed feature 8 (C18:1ω7c and/or C18:1ω6c), and significant proportions of C18:0 and C19:0 cyclo ω8cis were also found. The major respiratory quinone was ubiquinone-10. The G+C content of the DNA was 60.3‒60.9 mol%. Phylogenetic analyses by studying sequence information on the housekeeping atpD, dnaK, glnII, gyrB, and recA genes as well as on 16S rRNA genes and the 16S-23S rDNA internal transcribed spacer region revealed that the strains grouped with members of the genus Rhizobium, with Rhizobium selenitireducens as their closest relative but formed a distinct lineage at the species level. This was confirmed by genomic DNA-DNA hybridization studies. These phenotypic, genotypic, and phylogenetic data strongly suggest that our isolates should be classified under a novel species of the genus Rhizobium. Thus, we propose the name Rhizobium naphthalenivorans sp. nov. to accommodate the novel isolates. The type strain is TSY03b(T) (= NBRC 107585T = KCTC 23252T).

  19. Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR

    Directory of Open Access Journals (Sweden)

    Reith Michael

    2008-03-01

    Full Text Available Abstract Background Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using quantitative real-time PCR (qRT-PCR technique. Therefore, adequate reference genes for accurate normalization are required. Results The stability of 12 potential reference genes was examined during larval development in Senegalese sole (Solea senegalensis and Atlantic halibut (Hippoglossus hippoglossus to determine the most suitable genes for qRT-PCR analysis. Transcription levels of genes encoding β-Actin (ACTB, glyceraldehyde-3P-dehydrogenase (GAPDH, annexin A2 (ANXA2, glutathione S-transferase (GST, ornithine decarboxylase (ODC, hypoxanthine phosphoribosyltransferase (HPRT1, ubiquitin (UBQ, elongation factor 1 alpha (eEF1A1, 18S ribosomal RNA, and the ribosomal proteins S4 (RPS4 and L13a (RPL13a were quantitated. Two paralogous genes for ACTB were analyzed in each of both flatfish species. In addition, two paralogous genes for GAPDH were studied in Senegalese sole. RPL13a represented non-orthologous genes between both flatfish species. GeNorm and NormFinder analyses for expression stability revealed RPS4, UBQ and eEF1A1 as the most stable genes in Senegalese sole, Atlantic halibut and in a combined analysis. In all cases, paralogous genes exhibited differences in expression stability. Conclusion This work suggests RPS4, UBQ, and eEF1A1 genes as useful reference genes for accurate normalization in qRT-PCR studies in Senegalese sole and Atlantic halibut larvae. The congruent results between both species in spite of the drastic differences in larval development suggest that selected housekeeping genes (HKGs could be useful in other flatfish species. However, the finding of paralogous gene copies differentially expressed during development in some HKGs underscores the necessity to

  20. Decay data file based on the ENSDF file

    Energy Technology Data Exchange (ETDEWEB)

    Katakura, J. [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1997-03-01

    A decay data file with the JENDL (Japanese Evaluated Nuclear Data Library) format based on the ENSDF (Evaluated Nuclear Structure Data File) file was produced as a tentative one of special purpose files of JENDL. The problem using the ENSDF file as primary source data of the JENDL decay data file is presented. (author)

  1. Structural Differences between the Streptococcus agalactiae Housekeeping and Pilus-Specific Sortases: SrtA and SrtC1

    Energy Technology Data Exchange (ETDEWEB)

    Khare, B.; Krishnan, V.; Rajashankar, K.R.; I-Hsiu, H.; Xin, M.; Ton-That, H.; Narayana, S.V. (Texas-HSC); (Cornell); (UAB)

    2011-10-21

    The assembly of pili on the cell wall of Gram-positive bacteria requires transpeptidase enzymes called sortases. In Streptococcus agalactiae, the PI-1 pilus island of strain 2603V/R encodes two pilus-specific sortases (SrtC1 and SrtC2) and three pilins (GBS80, GBS52 and GBS104). Although either pilus-specific sortase is sufficient for the polymerization of the major pilin, GBS80, incorporation of the minor pilins GBS52 and GBS104 into the pilus structure requires SrtC1 and SrtC2, respectively. The S. agalactiae housekeeping sortase, SrtA, whose gene is present at a different location and does not catalyze pilus polymerization, was shown to be involved in cell wall anchoring of pilus polymers. To understand the structural basis of sortases involved in such diverse functions, we determined the crystal structures of S. agalactiae SrtC1 and SrtA. Both enzymes are made of an eight-stranded beta-barrel core with variations in their active site architecture. SrtA exhibits a catalytic triad arrangement similar to that in Streptococcus pyogenes SrtA but different from that in Staphylococcus aureus SrtA. In contrast, the SrtC1 enzyme contains an N-terminal helical domain and a 'lid' in its putative active site, which is similar to that seen in Streptococcus pneumoniae pilus-specific sortases, although with subtle differences in positioning and composition. To understand the effect of such differences on substrate recognition, we have also determined the crystal structure of a SrtC1 mutant, in which the conserved DP(W/F/Y) motif was replaced with the sorting signal motif of GBS80, IPNTG. By comparing the structures of WT wild type SrtA and SrtC1 and the 'lid' mutant of SrtC1, we propose that structural elements within the active site and the lid may be important for defining the role of specific sortase in pili biogenesis.

  2. Phylogenetic-signal dissection of nuclear housekeeping genes supports the paraphyly of sponges and the monophyly of Eumetazoa.

    Science.gov (United States)

    Sperling, Erik A; Peterson, Kevin J; Pisani, Davide

    2009-10-01

    The relationships at the base of the metazoan tree have been difficult to robustly resolve, and there are several different hypotheses regarding the interrelationships among sponges, cnidarians, ctenophores, placozoans, and bilaterians, with each hypothesis having different implications for the body plan of the last common ancestor of animals and the paleoecology of the late Precambrian. We have sequenced seven nuclear housekeeping genes from 17 new sponges, bringing the total to 29 species analyzed, including multiple representatives of the Demospongiae, Calcarea, Hexactinellida, and Homoscleromorpha, and analyzed a data set also including six nonmetazoan outgroups and 36 eumetazoans using a variety of phylogenetic methods and evolutionary models. We used leaf stability to identify rogue taxa and investigate their effect on the support of the nodes in our trees, and we identified clades most likely to represent phylogenetic artifacts through the comparison of trees derived using different methods (and models) and through site-stripping analyses. Further, we investigated compositional heterogeneity and tested whether amino acid composition bias affected our results. Finally, we used Bayes factors to compare our results against previously published phylogenies. All our maximum likelihood (ML) and Bayesian analyses find sponges to be paraphyletic, with all analyses finding three extant paraphyletic sponge lineages, Demospongiae plus Hexactinellida, Calcarea, and Homoscleromorpha. All but one of our ML and Bayesian analyses support the monophyly of Eumetazoa (here Cnidaria + Bilateria) and a sister group relationship between Placozoa (here Trichoplax adhaerens) and Eumetazoa. Bayes factors invariably provide decisive support in favor of poriferan paraphyly when compared against either a sister group relationship between Porifera and Cnidaria or with a monophyletic Porifera with respect to a monophyletic Eumetazoa. Although we were able to recover sponge monophyly

  3. Structure and specificity of a new class of Ca(2+) independent housekeeping sortase from Streptomyces avermitilis provides insights into its non-canonical substrate preference.

    Science.gov (United States)

    Das, Sreetama; Pawale, Vijaykumar S; Dadireddy, Venkatareddy; Singh, Avinash Kumar; Ramakumar, Suryanarayanarao; Roy, Rajendra P

    2017-03-07

    Surface proteins in Gram-positive bacteria are incorporated into the cell wall through a peptide ligation reaction catalyzed by transpeptidase sortase. Six main classes (A-F) of sortase have been identified of which class A sortase is meant for housekeeping functions. The prototypic housekeeping sortase A (SaSrtA) from Staphylococcus aureus cleaves LPXTG-containing proteins at the scissile T-G peptide bond and ligates Protein-LPXT to the terminal Gly residue of the nascent cross-bridge of peptidoglycan Lipid II precursor. Sortase-mediated ligation ('sortagging') of LPXTG-containing substrates and Gly-terminated nucleophiles occurs in vitro as well as in cellulo in the presence of Ca(2+) and has been applied extensively for protein conjugations. Although majority of applications emanate from SaSrtA, low catalytic efficiency, LPXTG specificity restriction, and Ca(2+) requirement (particularly for in cellulo applications) remains a drawback. Given that Gram-positive bacterial genomes encode a variety of sortases, natural sortase mining can be a viable complementary approach akin to engineering of wild type SaSrtA. Here we describe the structure and specificity of a new class E sortase (SavSrtE) annotated to perform housekeeping roles in Streptomyces avermitilis Biochemical experiments define the attributes of an optimum peptide substrate, demonstrate Ca(2+)-independent activity and provide insights about contrasting functional characteristics of SavSrtE and SaSrtA. Crystal structure, substrate docking and mutagenesis experiments have identified a critical residue that dictates the preference for a non-canonical LAXTG recognition motif over LPXTG. These results have implications for rational tailoring of substrate tolerance in sortases. Besides, Ca(2+) independent orthogonal specificity of SavSrtE is likely to expand the sortagging toolkit.

  4. Standard interface file handbook

    Energy Technology Data Exchange (ETDEWEB)

    Shapiro, A.; Huria, H.C. (Cincinnati Univ., OH (United States))

    1992-10-01

    This handbook documents many of the standard interface file formats that have been adopted by the US Department of Energy to facilitate communications between and portability of, various large reactor physics and radiation transport software packages. The emphasis is on those files needed for use of the VENTURE/PC diffusion-depletion code system. File structures, contents and some practical advice on use of the various files are provided.

  5. PCF File Format.

    Energy Technology Data Exchange (ETDEWEB)

    Thoreson, Gregory G [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-08-01

    PCF files are binary files designed to contain gamma spectra and neutron count rates from radiation sensors. It is the native format for the GAmma Detector Response and Analysis Software (GADRAS) package [1]. It can contain multiple spectra and information about each spectrum such as energy calibration. This document outlines the format of the file that would allow one to write a computer program to parse and write such files.

  6. 43 CFR 4.1381 - Who may file; when to file; where to file.

    Science.gov (United States)

    2010-10-01

    ... may file; when to file; where to file. (a) Any person who receives a written decision issued by OSM under 30 CFR 773.28 on a challenge to an ownership or control listing or finding may file a request for... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file; when to file; where to...

  7. On a family of KP multi-line solitons associated to rational degenerations of real hyperelliptic curves and to the finite non-periodic Toda hierarchy

    Science.gov (United States)

    Abenda, Simonetta

    2017-09-01

    We continue the program started in Abenda and Grinevich (2015) of associating rational degenerations of M-curves to points in GrTNN(k , n) using KP theory for real finite gap solutions. More precisely, we focus on the inverse problem of characterizing the soliton data which produce Krichever divisors compatible with the KP reality condition when Γ is a certain rational degeneration of a hyperelliptic M-curve. Such choice is motivated by the fact that Γ is related to the curves associated to points in GrTP(1 , n) and in GrTP(n - 1 , n) in Abenda and Grinevich (2015). We prove that the reality condition on the Krichever divisor on Γ singles out a special family of KP multi-line solitons (T-hyperelliptic solitons) in GrTP(k , n) , k ∈ [ n - 1 ] , naturally connected to the finite non-periodic Toda hierarchy. We discuss the relations between the algebraic-geometric description of KP T-hyperelliptic solitons and of the open Toda system. Finally, we also explain the effect of the space-time transformation which conjugates soliton data in GrTP(k , n) to soliton data in GrTP(n - k , n) on the Krichever divisor for such KP solitons.

  8. Bayesian reliability analysis for non-periodic inspection with estimation of uncertain parameters; Bayesian shinraisei kaiseki wo tekiyoshita hiteiki kozo kensa ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Itagaki, H. [Yokohama National University, Yokohama (Japan). Faculty of Engineering; Asada, H.; Ito, S. [National Aerospace Laboratory, Tokyo (Japan); Shinozuka, M.

    1996-12-31

    Risk assessed structural positions in a pressurized fuselage of a transport-type aircraft applied with damage tolerance design are taken up as the subject of discussion. A small number of data obtained from inspections on the positions was used to discuss the Bayesian reliability analysis that can estimate also a proper non-periodic inspection schedule, while estimating proper values for uncertain factors. As a result, time period of generating fatigue cracks was determined according to procedure of detailed visual inspections. The analysis method was found capable of estimating values that are thought reasonable and the proper inspection schedule using these values, in spite of placing the fatigue crack progress expression in a very simple form and estimating both factors as the uncertain factors. Thus, the present analysis method was verified of its effectiveness. This study has discussed at the same time the structural positions, modeling of fatigue cracks generated and develop in the positions, conditions for destruction, damage factors, and capability of the inspection from different viewpoints. This reliability analysis method is thought effective also on such other structures as offshore structures. 18 refs., 8 figs., 1 tab.

  9. Validation of housekeeping genes as an internal control for gene expression studies in Giardia lamblia using quantitative real-time PCR.

    Science.gov (United States)

    Marcial-Quino, Jaime; Fierro, Francisco; De la Mora-De la Mora, Ignacio; Enríquez-Flores, Sergio; Gómez-Manzo, Saúl; Vanoye-Carlo, America; Garcia-Torres, Itzhel; Sierra-Palacios, Edgar; Reyes-Vivas, Horacio

    2016-04-25

    The analysis of transcript levels of specific genes is important for understanding transcriptional regulation and for the characterization of gene function. Real-time quantitative reverse transcriptase PCR (RT-qPCR) has become a powerful tool to quantify gene expression. The objective of this study was to identify reliable housekeeping genes in Giardia lamblia. Twelve genes were selected for this purpose, and their expression was analyzed in the wild type WB strain and in two strains with resistance to nitazoxanide (NTZ) and metronidazole (MTZ), respectively. RefFinder software analysis showed that the expression of the genes is different in the three strains. The integrated data from the four analyses showed that the NADH oxidase (NADH) and aldolase (ALD) genes were the most steadily expressed genes, whereas the glyceraldehyde-3-phosphate dehydrogenase gene was the most unstable. Additionally, the relative expression of seven genes were quantified in the NTZ- and MTZ-resistant strains by RT-qPCR, using the aldolase gene as the internal control, and the results showed a consistent differential pattern of expression in both strains. The housekeeping genes found in this work will facilitate the analysis of mRNA expression levels of other genes of interest in G. lamblia.

  10. Screening and Validation of Housekeeping Genes of the Root and Cotyledon of Cunninghamia lanceolata under Abiotic Stresses by Using Quantitative Real-Time PCR

    Directory of Open Access Journals (Sweden)

    Wenlong Bao

    2016-07-01

    Full Text Available Cunninghamia lanceolata (Chinese fir is a fast-growing and commercially important conifer of the Cupressaceae family. Due to the unavailability of complete genome sequences and relatively poor genetic background information of the Chinese fir, it is necessary to identify and analyze the expression levels of suitable housekeeping genes (HKGs as internal reference for precise analysis. Based on the results of database analysis and transcriptome sequencing, we have chosen five candidate HKGs (Actin, GAPDH, EF1a, 18S rRNA, and UBQ with conservative sequences in the Chinese fir and related species for quantitative analysis. The expression levels of these HKGs in roots and cotyledons under five different abiotic stresses in different time intervals were measured by qRT-PCR. The data were statistically analyzed using the following algorithms: NormFinder, BestKeeper, and geNorm. Finally, RankAggreg was applied to merge the sequences generated from three programs and rank these according to consensus sequences. The expression levels of these HKGs showed variable stabilities under different abiotic stresses. Among these, Actin was the most stable internal control in root, and GAPDH was the most stable housekeeping gene in cotyledon. We have also described an experimental procedure for selecting HKGs based on the de novo sequencing database of other non-model plants.

  11. 高星级酒店客房部绩效考核浅析%Analysis of High-star Hotel Housekeeping Performance Appraisal

    Institute of Scientific and Technical Information of China (English)

    吕伟成

    2013-01-01

    酒店业在快速发展的同时,对员工绩效的考核就变得越来越重要。本人在苏州某酒店客房部挂职期间,发现员工绩效考评中出现的种种不合理状况。本文针对这类状况做了探究分析,全方位对客房部员工的绩效考评的合理性、公平性提供了新策略。%With the rapid development of hotel industry, the employee performance appraisal becomes increasingly important. When taking a temporary post in housekeeping of one Suzhou hotel, the author found unreasonable situations in staff performance appraisal. This paper analyzed and explored the situations, and provided new strategies for the reasonableness and fairness of housekeeping staff performance appraisal.

  12. Identifiable Data Files - Name and Address File and Vital...

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Names and Addresses File and the Vital Status File are subsets of the data elements in the Enrollment Database (EDB). The particular information in each file is...

  13. Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia

    Directory of Open Access Journals (Sweden)

    Serena Joaquín

    2009-06-01

    Full Text Available Abstract Background Studies of gene expression in experimental cerebral ischaemia models can contribute to understanding the pathophysiology of brain ischaemia and to identifying prognostic markers and potential therapeutic targets. The normalization of relative qRT-PCR data using a suitable reference gene is a crucial prerequisite for obtaining reliable conclusions. No validated housekeeping genes have been reported for the relative quantification of the mRNA expression profile activated in in-vitro ischaemic conditions, whereas for the in-vivo model different reference genes have been used. The present study aims to determine the expression stability of ten housekeeping genes (Gapdh, β2m, Hprt, Ppia, Rpl13a, Oaz1, 18S rRNA, Gusb, Ywhaz and Sdha to establish their suitability as control genes for in-vitro and in-vivo cerebral ischaemia models. Results The expression stability of the candidate reference genes was evaluated using the 2-ΔC'T method and ANOVA followed by Dunnett's test. For the in-vitro model using primary cultures of rat astrocytes, all genes analysed except for Rpl13a and Sdha were found to have significantly different levels of mRNA expression. These different levels were also found in the case of the in-vivo model of pMCAO in rats except for Hprt, Sdha and Ywhaz mRNA, where the expression did not vary. Sdha and Ywhaz were identified by geNorm and NormFinder as the two most stable genes. Conclusion We have validated endogenous control genes for qRT-PCR analysis of gene expression in in-vitro and in-vivo cerebral ischaemia models. For normalization purposes, Rpl13a and Sdha are found to be the most suitable genes for the in-vitro model and Sdha and Ywhaz for the in-vivo model. Genes previously used as housekeeping genes for the in-vivo model in the literature were not validated as good control genes in the present study, showing the need for careful evaluation for each new experimental setup.

  14. Validation of housekeeping genes as internal controls for studying gene expression during Pacific oyster (Crassostrea gigas) development by quantitative real-time PCR.

    Science.gov (United States)

    Du, Yishuai; Zhang, Linlin; Xu, Fei; Huang, Baoyu; Zhang, Guofan; Li, Li

    2013-03-01

    Hatchery-reared larvae of the Pacific oyster (Crassostrea gigas) often suffer from massive mortality induced by Ostreid herpesvirus 1 (OsHV-1) infection, indicating the importance of better understanding of oyster immune defense systems. The accuracy of measurements of gene expression levels based on quantitative real-time PCR assays relies on the use of housekeeping genes as internal controls; however, few studies have focused on the selection of such internal controls. In this study, we conducted a comprehensive investigation of internal control genes during oyster development in virus-infected and uninfected samples. Transcriptome data for 38 developmental stages were downloaded and the gene expression patterns were classified into 30 clusters. A total of 317 orthologs of classical housekeeping genes in the oyster genome were annotated. After combining the expression profiles and oyster housekeeping gene dataset, 14 candidate internal controls were selected for further investigation: Elongation factor-1α (EF-1α), 18S rRNA (18S), 28S rRNA (28S), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin (ACT), Ribosomal protein L7 (RL7), Ribosomal protein L27 (RL27), Ribosomal protein L36 (RL36), Ribosomal protein S18 (RS18), Heterogeneous nuclear ribonucleoprotein A2/B1 (RO21), Eukaryotic translation elongation factor 2 (EF2), Ubiquitin-conjugating enzyme E2D2 (UBCD1), S-phase kinase-associated protein 1 (SKP1) and Heterogeneous nuclear ribonucleoprotein Q (HNRPQ). RNA was extracted from oyster larvae infected with OsHV-1 (group A; GA), and OsHV-1 free larvae (group B; GB). The expression levels of the 14 candidate internal controls were studied in GA and GB larvae by real-time PCR. Their expression stabilities were further analyzed using the GeNorm program. RL7 and RS18 were the most stable genes in both OsHV-1 infected (GA) and uninfected (GB) larvae. These results suggest that RL7 and RS18 could be used as internal controls for studying gene expression in

  15. Patient Assessment File (PAF)

    Data.gov (United States)

    Department of Veterans Affairs — The Patient Assessment File (PAF) database compiles the results of the Patient Assessment Instrument (PAI) questionnaire filled out for intermediate care Veterans...

  16. Sensitivity Data File Formats

    Energy Technology Data Exchange (ETDEWEB)

    Rearden, Bradley T. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-04-01

    The format of the TSUNAMI-A sensitivity data file produced by SAMS for cases with deterministic transport solutions is given in Table 6.3.A.1. The occurrence of each entry in the data file is followed by an identification of the data contained on each line of the file and the FORTRAN edit descriptor denoting the format of each line. A brief description of each line is also presented. A sample of the TSUNAMI-A data file for the Flattop-25 sample problem is provided in Figure 6.3.A.1. Here, only two profiles out of the 130 computed are shown.

  17. RRB Earnings File (RRBERN)

    Data.gov (United States)

    Social Security Administration — RRBERN contains records for all beneficiaries on the RRB's PSSVES file who's SSNs are validated through the SVES processing. Validated output is processed through...

  18. Register file soft error recovery

    Science.gov (United States)

    Fleischer, Bruce M.; Fox, Thomas W.; Wait, Charles D.; Muff, Adam J.; Watson, III, Alfred T.

    2013-10-15

    Register file soft error recovery including a system that includes a first register file and a second register file that mirrors the first register file. The system also includes an arithmetic pipeline for receiving data read from the first register file, and error detection circuitry to detect whether the data read from the first register file includes corrupted data. The system further includes error recovery circuitry to insert an error recovery instruction into the arithmetic pipeline in response to detecting the corrupted data. The inserted error recovery instruction replaces the corrupted data in the first register file with a copy of the data from the second register file.

  19. Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2016-01-01

    Full Text Available A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identification by 16S rRNA sequencing suggested that the isolate is R. terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpoB, gyrA, and parC with phylogenetic analysis. To our knowledge, this is the first report of subungual abscess caused by R. terrigena, a rare case of human infection due to soil bacterium. Our study highlights the technique importance on this pathogen identification.

  20. Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing.

    Science.gov (United States)

    Wang, Yu; Jiang, Xiawei; Xu, Zemin; Ying, Chaoqun; Yu, Wei; Xiao, Yonghong

    2016-01-01

    A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identification by 16S rRNA sequencing suggested that the isolate is R. terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpoB, gyrA, and parC) with phylogenetic analysis. To our knowledge, this is the first report of subungual abscess caused by R. terrigena, a rare case of human infection due to soil bacterium. Our study highlights the technique importance on this pathogen identification.

  1. Phylogenetic analyses and characterization of RNase X25 from Drosophila melanogaster suggest a conserved housekeeping role and additional functions for RNase T2 enzymes in protostomes.

    Directory of Open Access Journals (Sweden)

    Linda Ambrosio

    Full Text Available Ribonucleases belonging to the RNase T2 family are enzymes associated with the secretory pathway that are almost absolutely conserved in all eukaryotes. Studies in plants and vertebrates suggest they have an important housekeeping function in rRNA recycling. However, little is known about this family of enzymes in protostomes. We characterized RNase X25, the only RNase T2 enzyme in Drosophila melanogaster. We found that RNase X25 is the major contributor of ribonuclease activity in flies as detected by in gel assays, and has an acidic pH preference. Gene expression analyses showed that the RNase X25 transcript is present in all adult tissues and developmental stages. RNase X25 expression is elevated in response to nutritional stresses; consistent with the hypothesis that this enzyme has a housekeeping role in recycling RNA. A correlation between induction of RNase X25 expression and autophagy was observed. Moreover, induction of gene expression was triggered by oxidative stress suggesting that RNase X25 may have additional roles in stress responses. Phylogenetic analyses of this family in protostomes showed that RNase T2 genes have undergone duplication events followed by divergence in several phyla, including the loss of catalytic residues, and suggest that RNase T2 proteins have acquired novel functions. Among those, it is likely that a role in host immunosuppression evolved independently in several groups, including parasitic Platyhelminthes and parasitoid wasps. The presence of only one RNase T2 gene in the D. melanogaster genome, without any other evident secretory RNase activity detected, makes this organism an ideal system to study the cellular functions of RNase T2 proteins associated with RNA recycling and maintenance of cellular homeostasis. On the other hand, the discovery of gene duplications in several protostome genomes also presents interesting new avenues to study additional biological functions of this ancient family of proteins.

  2. Phylogenetic analyses and characterization of RNase X25 from Drosophila melanogaster suggest a conserved housekeeping role and additional functions for RNase T2 enzymes in protostomes.

    Science.gov (United States)

    Ambrosio, Linda; Morriss, Stephanie; Riaz, Ayesha; Bailey, Ryan; Ding, Jian; MacIntosh, Gustavo C

    2014-01-01

    Ribonucleases belonging to the RNase T2 family are enzymes associated with the secretory pathway that are almost absolutely conserved in all eukaryotes. Studies in plants and vertebrates suggest they have an important housekeeping function in rRNA recycling. However, little is known about this family of enzymes in protostomes. We characterized RNase X25, the only RNase T2 enzyme in Drosophila melanogaster. We found that RNase X25 is the major contributor of ribonuclease activity in flies as detected by in gel assays, and has an acidic pH preference. Gene expression analyses showed that the RNase X25 transcript is present in all adult tissues and developmental stages. RNase X25 expression is elevated in response to nutritional stresses; consistent with the hypothesis that this enzyme has a housekeeping role in recycling RNA. A correlation between induction of RNase X25 expression and autophagy was observed. Moreover, induction of gene expression was triggered by oxidative stress suggesting that RNase X25 may have additional roles in stress responses. Phylogenetic analyses of this family in protostomes showed that RNase T2 genes have undergone duplication events followed by divergence in several phyla, including the loss of catalytic residues, and suggest that RNase T2 proteins have acquired novel functions. Among those, it is likely that a role in host immunosuppression evolved independently in several groups, including parasitic Platyhelminthes and parasitoid wasps. The presence of only one RNase T2 gene in the D. melanogaster genome, without any other evident secretory RNase activity detected, makes this organism an ideal system to study the cellular functions of RNase T2 proteins associated with RNA recycling and maintenance of cellular homeostasis. On the other hand, the discovery of gene duplications in several protostome genomes also presents interesting new avenues to study additional biological functions of this ancient family of proteins.

  3. FHEO Filed Cases

    Data.gov (United States)

    Department of Housing and Urban Development — The dataset is a list of all the Title VIII fair housing cases filed by FHEO from 1/1/2007 - 12/31/2012 including the case number, case name, filing date, state and...

  4. Targeted disruption of the housekeeping gene encoding glucose 6-phosphate dehydrogenase (G6PD-null): G6PD is dispensable for pentose synthesis but essential for defense against oxidative stress.

    NARCIS (Netherlands)

    P.P. Pandolfi; F. Sonati; R. Rivi; P. Mason; F.G. Grosveld (Frank); L. Luzzatto

    1995-01-01

    textabstractGlucose 6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme encoded in mammals by an X-linked gene. It has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH. In h

  5. Selective File Dumper

    Science.gov (United States)

    Bassetti, Nanni; Frati, Denis

    During a computer forensics investigation we faced a problem how to get all the interesting files we need fast. We work, mainly, using the Open Source software products and Linux OS, and we consider the Sleuthkit and the Foremost two very useful tools, but for reaching our target they were too complicated and time consuming to use. For this reason we developed the Selective File Dumper, a Linux Bash script which makes it possible to extract all the referenced, deleted and unallocated files and finally to perform a keyword search, in a simple way.

  6. Value-Based File Retention: File Attributes as File Value and Information Waste Indicators

    NARCIS (Netherlands)

    Wijnhoven, Fons; Amrit, Chintan; Dietz, Pim

    2014-01-01

    Several file retention policy methods propose that a file retention policy should be based on file value. Though such a retention policy might increase the value of accessible files, the method to arrive at such a policy is underresearched. This article discusses how one can arrive at a method for d

  7. Closed Claim Query File

    Data.gov (United States)

    Social Security Administration — This file is used to hold information about disability claims that have been closed and have been selected for sampling.Sampling is the process whereby OQR reviews...

  8. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file contains data on characteristics of hospitals and other types of healthcare facilities, including the name and address of the facility and the type of...

  9. MMLEADS Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare-Medicaid Linked Enrollee Analytic Data Source (MMLEADS) Public Use File (PUF) contains demographic, enrollment, condition prevalence, utilization, and...

  10. Patient Treatment File (PTF)

    Data.gov (United States)

    Department of Veterans Affairs — This database is part of the National Medical Information System (NMIS). The Patient Treatment File (PTF) contains a record for each inpatient care episode provided...

  11. USEEIO Satellite Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — These files contain the environmental data as particular emissions or resources associated with a BEA sectors that are used in the USEEIO model. They are organized...

  12. Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

    Directory of Open Access Journals (Sweden)

    Barbara Bennani-Baiti

    Full Text Available Endothelial cells are often present at inflammation sites. This is the case of endothelial cells of the blood-brain barrier (BBB of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases of bacterial meningitis, trauma, or tumor-associated ischemia. Inflammation is a known modulator of gene expression through the activation of transcription factors, mostly NF-κB. RLIP76 (a.k.a. RALBP1, an ATP-dependent transporter of electrophile-glutathione conjugates, modulates BBB permeability through the regulation of tight junction function, cell adhesion, and exocytosis. Genes and pathways regulated by RLIP76 are transcriptional targets of tumor necrosis factor alpha (TNF-α pro-inflammatory molecule, suggesting that RLIP76 may also be an inflammation target. To assess the effects of TNF-α on RLIP76, we faced the problem of choosing reference genes impervious to TNF-α. Since such genes were not known in human BBB endothelial cells, we subjected these to TNF-α, and measured by quantitative RT-PCR the expression of housekeeping genes commonly used as reference genes. We find most to be modulated, and analysis of several inflammation datasets as well as a metaanalysis of more than 5000 human tissue samples encompassing more than 300 cell types and diseases show that no single housekeeping gene may be used as a reference gene. Using three different algorithms, however, we uncovered a reference geneset impervious to TNF-α, and show for the first time that RLIP76 expression is induced by TNF-α and follows the induction kinetics of inflammation markers, suggesting that inflammation can influence RLIP76 expression at the BBB. We also show that MRP1 (a.k.a. ABCC1, another electrophile-glutathione transporter, is not modulated in the same cells and conditions, indicating that RLIP76 regulation by TNF-α is not a general property of glutathione transporters. The reference geneset

  13. Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.

    Science.gov (United States)

    Bennani-Baiti, Barbara; Toegel, Stefan; Viernstein, Helmut; Urban, Ernst; Noe, Christian R; Bennani-Baiti, Idriss M

    2015-01-01

    Endothelial cells are often present at inflammation sites. This is the case of endothelial cells of the blood-brain barrier (BBB) of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases of bacterial meningitis, trauma, or tumor-associated ischemia. Inflammation is a known modulator of gene expression through the activation of transcription factors, mostly NF-κB. RLIP76 (a.k.a. RALBP1), an ATP-dependent transporter of electrophile-glutathione conjugates, modulates BBB permeability through the regulation of tight junction function, cell adhesion, and exocytosis. Genes and pathways regulated by RLIP76 are transcriptional targets of tumor necrosis factor alpha (TNF-α) pro-inflammatory molecule, suggesting that RLIP76 may also be an inflammation target. To assess the effects of TNF-α on RLIP76, we faced the problem of choosing reference genes impervious to TNF-α. Since such genes were not known in human BBB endothelial cells, we subjected these to TNF-α, and measured by quantitative RT-PCR the expression of housekeeping genes commonly used as reference genes. We find most to be modulated, and analysis of several inflammation datasets as well as a metaanalysis of more than 5000 human tissue samples encompassing more than 300 cell types and diseases show that no single housekeeping gene may be used as a reference gene. Using three different algorithms, however, we uncovered a reference geneset impervious to TNF-α, and show for the first time that RLIP76 expression is induced by TNF-α and follows the induction kinetics of inflammation markers, suggesting that inflammation can influence RLIP76 expression at the BBB. We also show that MRP1 (a.k.a. ABCC1), another electrophile-glutathione transporter, is not modulated in the same cells and conditions, indicating that RLIP76 regulation by TNF-α is not a general property of glutathione transporters. The reference geneset uncovered herein should

  14. Closure Report for Corrective Action Unit 548: Areas 9, 10, 18, 19, and 20 Housekeeping Sites, Nevada National Security Site, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    NSTec Environmental Restoration

    2012-08-27

    This Closure Report (CR) documents closure activities for Corrective Action Unit (CAU) 548, Areas 9, 10, 18, 19, and 20 Housekeeping Sites, and complies with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the State of Nevada; the U.S. Department of Energy (DOE), Environmental Management; the U.S. Department of Defense; and DOE, Legacy Management (FFACO, 1996 as amended). CAU 548 consists of the following Corrective Action Sites (CASs), located in Areas 9, 10, 12, 18, 19, and 20 of the Nevada National Security Site: · CAS 09-99-02, Material Piles (2) · CAS 09-99-04, Wax, Paraffin · CAS 09-99-05, Asbestos, Vermiculite · CAS 09-99-07, Tar Spill · CAS 10-22-02, Drums · CAS 10-22-05, Gas Block · CAS 10-22-07, Gas Block · CAS 10-22-34, Drum · CAS 10-22-38, Drum; Cable · CAS 12-99-04, Epoxy Tar Spill · CAS 12-99-08, Cement Spill · CAS 18-14-01, Transformers (3) · CAS 19-22-01, Drums · CAS 19-22-11, Gas Block (2) · CAS 19-44-01, Fuel Spill · CAS 20-22-07, Drums (2) · CAS 20-22-09, Drums (3) · CAS 20-22-14, Drums (2) · CAS 20-22-16, Drums (2) · CAS 20-24-09, Battery Closure activities began in July 2011 and were completed in December 2011 and included removal and disposal of material piles, spills, sanitary debris, a lead acid battery, lead and steel shot, and stained soil. Activities were conducted according to the Sectored Clean-up Work Plan for Housekeeping Category Waste Sites (U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office [NNSA/NSO], 2003). Closure activities generated sanitary waste, hydrocarbon waste, low-level waste, hazardous waste, and mixed waste. Some wastes exceeded land disposal limits and required offsite treatment prior to disposal. Other wastes met land disposal restrictions and were disposed in appropriate onsite or offsite landfills. NNSA/NSO requests the following: · A Notice of Completion from the Nevada Division of Environmental Protection to NNSA/NSO for

  15. File System Virtual Appliances

    Science.gov (United States)

    2010-05-01

    some of my closest friendships. Ashraf, Ippo and Neil made life much more fun. Mike Merideth introduced me to fine scotch, hi-fi speakers, and piano ...system virtual appliances (4) We analyze the sources of latency in traditional inter-VM communica- tion techniques and present a novel energy- and...tiple file system implementations within the Sun UNIX kernel” [52]. This was achieved through two techniques . First, outside the file system layer

  16. INTERNET: PENGIRIMAN FILE

    Directory of Open Access Journals (Sweden)

    Zainul Bakri

    2012-10-01

    Full Text Available Salah satu alasan pengguna komputer untuk berhubungan dengan Internet adalah mendapat kesempatan untuk menyalin ('download' informasi yang tersimpan dari server jaringan komputer lain (misalnya menyalin program aplikasi komputer, data mentah, dan sebagainya. File Transfer  Protocol (FfP adalah cara di Internet untuk mengirim file dari satu tempat ke komputer pengguna. Untuk keperluan ini dapat menggunakan program FTP khusus atau dengan menggunakan Web browser.

  17. Identification and analysis of house-keeping and tissue-specific genes based on RNA-seq data sets across 15 mouse tissues.

    Science.gov (United States)

    Zeng, Jingyao; Liu, Shoucheng; Zhao, Yuhui; Tan, Xinyu; Aljohi, Hasan Awad; Liu, Wanfei; Hu, Songnian

    2016-01-15

    Recently, RNA-seq has become widely used technology for transcriptome profiling due to its single-base accuracy and high-throughput speciality. In this study, we applied a computational approach on an integrated RNA-seq dataset across 15 normal mouse tissues, and consequently assigned 8408 house-keeping (HK) genes and 2581 tissue-specific (TS) genes among UCSC RefGene annotation. Apart from some basic genomic features, we also performed expression, function and pathway analysis with clustering, DAVID and Ingenuity Pathway Analysis, indicating the physiological connections (tissues) and diverse biological roles of HK genes (fundamental processes) and TS genes (tissue-corresponding processes). Moreover, we used RT-PCR method to test 18 candidate HK genes and finally identified a novel list of highly stable internal control genes: Ywhae, Ddb 1, Eif4h, etc. In summary, this study provides a new HK gene and TS gene resource for further genetic and evolution research and helps us better understand morphogenesis and biological diversity in mouse.

  18. DSSTOX MASTER STRUCTURE-INDEX FILE: SDF FILE AND DOCUMENTATION

    Science.gov (United States)

    The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website.

  19. DSSTOX MASTER STRUCTURE-INDEX FILE: SDF FILE AND ...

    Science.gov (United States)

    The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website. The DSSTox Master Structure-Index File serves to consolidate, manage, and ensure quality and uniformity of the chemical and substance information spanning all DSSTox Structure Data Files, including those in development but not yet published separately on this website.

  20. Chemical genetic screen identifies lithocholic acid as an anti-aging compound that extends yeast chronological life span in a TOR-independent manner, by modulating housekeeping longevity assurance processes

    Science.gov (United States)

    Goldberg, Alexander A.; Richard, Vincent R.; Kyryakov, Pavlo; Bourque, Simon D.; Beach, Adam; Burstein, Michelle T.; Glebov, Anastasia; Koupaki, Olivia; Boukh-Viner, Tatiana; Gregg, Christopher; Juneau, Mylène; English, Ann M.; Thomas, David Y.; Titorenko, Vladimir I.

    2010-01-01

    In chronologically aging yeast, longevity can be extended by administering a caloric restriction (CR) diet or some small molecules. These life-extending interventions target the adaptable target of rapamycin (TOR) and cAMP/protein kinase A (cAMP/PKA) signaling pathways that are under the stringent control of calorie availability. We designed a chemical genetic screen for small molecules that increase the chronological life span of yeast under CR by targeting lipid metabolism and modulating housekeeping longevity pathways that regulate longevity irrespective of the number of available calories. Our screen identifies lithocholic acid (LCA) as one of such molecules. We reveal two mechanisms underlying the life-extending effect of LCA in chronologically aging yeast. One mechanism operates in a calorie availability-independent fashion and involves the LCA-governed modulation of housekeeping longevity assurance pathways that do not overlap with the adaptable TOR and cAMP/PKA pathways. The other mechanism extends yeast longevity under non-CR conditions and consists in LCA-driven unmasking of the previously unknown anti-aging potential of PKA. We provide evidence that LCA modulates housekeeping longevity assurance pathways by suppressing lipid-induced necrosis, attenuating mitochondrial fragmentation, altering oxidation-reduction processes in mitochondria, enhancing resistance to oxidative and thermal stresses, suppressing mitochondria-controlled apoptosis, and enhancing stability of nuclear and mitochondrial DNA. PMID:20622262

  1. Product identification file

    Energy Technology Data Exchange (ETDEWEB)

    Gray, C.E. (ed.)

    1978-06-01

    This product identification file was compiled as an aid to the industrial hygienist who may encounter the products listed in surveys for and studies of occupational health hazards. It is pointed out that the chemical composition of a product may vary from year to year and some components may be added or deleted without an indication on the label. Some of the data in this file may not be complete depending on the analysis requested. For example, a solvent may be the only component for which the product was analyzed. The file is arranged by listing the chemical manufacturer, followed by the trade name. In cases where no manufacturer is known, the trade name appears in alphabetical order. The log number and the chemist who analyzed the product are listed for reference.

  2. Instabilities in fluid layers and in reaction-diffusion systems: Steady states, time-periodic solutions, non-periodic attractors, and related convective and otherwise non-linear phenomena

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Velarde, M.

    1977-07-01

    Thermo convective instabilities in horizontal fluid layers are discussed with emphasis on the Rayleigh-Bernard model problem. Steady solutions and time-dependent phenomena (relaxation oscillations and transition to turbulence) are studied within the nonlinear Boussinesq-Oberbeck approximation. Homogeneous steady solutions, limit cycles, and inhomogeneous (ordered) spatial structures are also studied in simple reaction-diffusion systems. Lastly, the non-periodic attractor that appears at large Rayleigh numbers in the truncated Boussinesq-Oberbeck model of Lorenz, is constructed, and a discussion of turbulent behavior is given. (Author) 105 refs.

  3. Formalizing a Hierarchical File System

    NARCIS (Netherlands)

    Hesselink, Wim H.; Lali, M.I.

    2009-01-01

    In this note, we define an abstract file system as a partial function from (absolute) paths to data. Such a file system determines the set of valid paths. It allows the file system to be read and written at a valid path, and it allows the system to be modified by the Unix operations for removal (rm)

  4. 76 FR 39757 - Filing Procedures

    Science.gov (United States)

    2011-07-06

    ... an accurate filing history can be maintained. (b) Changes to user information other than the Firm... computer or internet resources. If the request is granted, the Secretary will promptly inform you and... applicable hardware and software requirements for electronic filing. (2) To file or submit a document in...

  5. Formalizing a hierarchical file system

    NARCIS (Netherlands)

    Hesselink, Wim H.; Lali, Muhammad Ikram

    2012-01-01

    An abstract file system is defined here as a partial function from (absolute) paths to data. Such a file system determines the set of valid paths. It allows the file system to be read and written at a valid path, and it allows the system to be modified by the Unix operations for creation, removal, a

  6. Selection of ovine housekeeping genes for normalisation by real-time RT-PCR; analysis of PrP gene expression and genetic susceptibility to scrapie

    Directory of Open Access Journals (Sweden)

    Hurtado Ana

    2005-09-01

    Full Text Available Abstract Background Cellular prion protein expression is essential for the development of transmissible spongiform encephalopathies (TSEs, and in sheep, genetic susceptibility to scrapie has been associated to PrP gene polymorphisms. To test the hypothetical linkage between PrP gene expression and genetic susceptibility, PrP mRNA levels were measured by real-time RT-PCR in six ovine tissues of animals with different genotypes. Results Previous to the PrP gene expression analysis the stability of several housekeeping (HK genes was assessed in order to select the best ones for relative quantification. The normalisation of gene expression was carried out using a minimum of three HK genes in order to detect small expression differences more accurately than using a single control gene. The expression stability analysis of six HK genes showed a large tissue-associated variation reflecting the existence of tissue-specific factors. Thereby, a specific set of HK genes was required for an accurate normalisation of the PrP gene expression within each tissue. Statistical differences in the normalised PrP mRNA levels were found among the tissues, obtaining the highest expression level in obex, followed by ileum, lymph node, spleen, cerebellum and cerebrum. A tendency towards increased PrP mRNA levels and genetic susceptibility was observed in central nervous system. However, the results did not support the hypothesis that PrP mRNA levels vary between genotypes. Conclusion The results on PrP gene expression presented here provide valuable baseline data for future studies on scrapie pathogenesis. On the other hand, the results on stability data of several HK genes reported in this study could prove very useful in other gene expression studies carried out in these relevant ovine tissues.

  7. ATP8B1 gene expression is driven by a housekeeping-like promoter independent of bile acids and farnesoid X receptor.

    Directory of Open Access Journals (Sweden)

    Dita Cebecauerová

    Full Text Available BACKGROUND: Mutations in ATP8B1 gene were identified as a cause of low γ-glutamyltranspeptidase cholestasis with variable phenotype, ranging from Progressive Familial Intrahepatic Cholestasis to Benign Recurrent Intrahepatic Cholestasis. However, only the coding region of ATP8B1 has been described. The aim of this research was to explore the regulatory regions, promoter and 5'untranslated region, of the ATP8B1 gene. METHODOLOGY/PRINCIPAL FINDINGS: 5'Rapid Amplification of cDNA Ends using human liver and intestinal tissue was performed to identify the presence of 5' untranslated exons. Expression levels of ATP8B1 transcripts were determined by quantitative reverse-transcription PCR and compared with the non-variable part of ATP8B1. Three putative promoters were examined in vitro using a reporter gene assay and the main promoter was stimulated with chenodeoxycholic acid. Four novel untranslated exons located up to 71 kb upstream of the previously published exon 1 and twelve different splicing variants were found both in the liver and the intestine. Multiple transcription start sites were identified within exon -3 and the proximal promoter upstream of this transcription start site cluster was proven to be an essential regulatory element responsible for 70% of total ATP8B1 transcriptional activity. In vitro analysis demonstrated that the main promoter drives constitutive ATP8B1 gene expression independent of bile acids. CONCLUSIONS/SIGNIFICANCE: The structure of the ATP8B1 gene is complex and the previously published transcription start site is not significant. The basal expression of ATP8B1 is driven by a housekeeping-like promoter located 71 kb upstream of the first protein coding exon.

  8. 43 CFR 4.1371 - Who may file, where to file, when to file.

    Science.gov (United States)

    2010-10-01

    ... of proposed suspension or rescission under 30 CFR 773.22 or a notice of suspension or rescission under 30 CFR 773.23 may file a request for review with the Hearings Division, Office of Hearings and... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file, where to file, when to...

  9. 78 FR 17394 - Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing...

    Science.gov (United States)

    2013-03-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing Process; Notice of Technical Conference Take notice that on April 16, 2013,...

  10. 11 CFR 100.19 - File, filed or filing (2 U.S.C. 434(a)).

    Science.gov (United States)

    2010-01-01

    ... a facsimile machine or by electronic mail if the reporting entity is not required to file..., including electronic reporting entities, may use the Commission's website's on-line program to file 48-hour... the reporting entity is not required to file electronically in accordance with 11 CFR 104.18....

  11. Discussion on Recovery Protection for HVDC Thyristor Valve in the Non-periodic Firing Tests%HVDC晶闸管换流阀非周期触发试验中恢复期保护的探讨

    Institute of Scientific and Technical Information of China (English)

    陆建挺; 杨涛; 申萌; 钟磊; 周敏刚

    2011-01-01

    The non-periodic firing test is an important test of valve type tests for HVDC thyristor valve. For a complete valve, parallel capacitor method is normally adopted for testing. Some issues about recovery protection in the non-periodic firing tests have been discussed in this paper. Tests show that after the thyristor triggered, in recovery period, over high Aid At or re-triggered the thyristor, this may cause damage to thyristor. Base on this, the appropriate recommendations are proposed.%高压直流输电HVDC晶闸管换流阀的非周期触发试验是换流阀型式试验中的一项重要试验.对于完整的阀,通常采用并联电容器法进行试验.笔者比较了几个不同工程晶闸管换流阀的试验数据,探讨了晶闸管非周期触发试验中恢复期保护的几个问题.实验证明,晶闸管触发后恢复期内过高的du/dt或再次触发将可能导致晶闸管的损害,并提出了相应的建议.

  12. Download this PDF file

    African Journals Online (AJOL)

    Buchi

    Method: The case files of the 225 maternal deaths which occurred between ... cause related to or aggravated by the pregnancy or its ... Multiple factors have been suggested for this fall ..... responsible for only 2, (0.9%) of deaths. In other.

  13. Download this PDF file

    African Journals Online (AJOL)

    User

    MARC (machine-readable catalogue) format. The records are ... software in the library, while in Web OPAC,. HTML files are used ... training in other fields of applied learning that is relevant to the ..... the Libraries of Engineering. Colleges in ...

  14. Download this PDF file

    African Journals Online (AJOL)

    Fr. Ikenga

    Nigeria; and the gains and challenges of utilizing e-taxation in tax ... teething problems usually encountered in all new schemes. ... is to help tax authorities reduce and possibly eliminate tax evasion. .... issues that include how to use the tax authorities' electronic tax filing system and ..... financial institution for Direct Deposit.

  15. The New Resource File

    Science.gov (United States)

    Luck, Donald D.

    2011-01-01

    The development of the resource file is a common experience in teacher preparation programs. The author examines strategies for transforming what has been a project composed of physical resources to one emphasizing digital resources. Methods for finding, tagging, storing and retrieving resources are explored.

  16. Permanent-File-Validation Utility Computer Program

    Science.gov (United States)

    Derry, Stephen D.

    1988-01-01

    Errors in files detected and corrected during operation. Permanent File Validation (PFVAL) utility computer program provides CDC CYBER NOS sites with mechanism to verify integrity of permanent file base. Locates and identifies permanent file errors in Mass Storage Table (MST) and Track Reservation Table (TRT), in permanent file catalog entries (PFC's) in permit sectors, and in disk sector linkage. All detected errors written to listing file and system and job day files. Program operates by reading system tables , catalog track, permit sectors, and disk linkage bytes to vaidate expected and actual file linkages. Used extensively to identify and locate errors in permanent files and enable online correction, reducing computer-system downtime.

  17. Huygens file service and storage architecture

    NARCIS (Netherlands)

    Bosch, Peter; Mullender, Sape; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage architectu

  18. Huygens file server and storage architecture

    NARCIS (Netherlands)

    Bosch, Peter; Mullender, Sape; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage architectu

  19. 12 CFR 5.4 - Filing required.

    Science.gov (United States)

    2010-01-01

    ... CORPORATE ACTIVITIES Rules of General Applicability § 5.4 Filing required. (a) Filing. A depository institution shall file an application or notice with the OCC to engage in corporate activities and... advise an applicant through a pre-filing communication to send the filing or submission directly to...

  20. PFS: A Distributed and Customizable File System

    NARCIS (Netherlands)

    Bosch, Peter

    1996-01-01

    In this paper we present our ongoing work on the Pegasus File System (PFS), a distributed and customizable file system that can be used for off-line file system experiments and on-line file system storage. PFS is best described as an object-oriented component library from which either a true file sy

  1. 43 CFR 4.1362 - Where to file; when to file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Where to file; when to file. 4.1362... file; when to file. (a) The request for review shall be filed with the Hearings Division, Office of... bear the expense for this service. (b) Failure to file a request for review within the time...

  2. JENDL fusion file 99

    Energy Technology Data Exchange (ETDEWEB)

    Chiba, Satoshi; Fukahori, Tokio; Shibata, Keiichi [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Yu Baosheng [China Institute of Atomic Energy, Beijing (China); Kosako, Kazuaki [Sumitomo Atomic Industries, Tokyo (Japan); Yamamuro, Nobuhiro [Data Engineering Co. Ltd., Yokohama, Kanagawa (Japan)

    2002-02-01

    The double-differential cross sections (DDXs) of secondary neutrons have been evaluated for 79 isotopes and 13 natural elements ranging from H to Bi to improve the accuracy of predictions for the neutronics calculations in the D-T thermonuclear fusion applications. The data given in JENDL-3.1, which was the newest version of JENDL general purpose file when this project was initiated, was combined with new calculations based on the optical model, DWBA, pre-equilibrium and multi-step statistical models, and the DDX data were generated based on various kinds of systematics for medium-mass nuclei. Different methods were employed for light nuclei to which the above method could not be applied. In addition, the DDXs for emission of charged particles (p, d, t, {sup 3}He and {alpha}-particle) were given for {sup 2}H, {sup 9}Be and elements heavier or equal to F. The present results give an overall good description of the measured DDX data of both the neutron and charged particles emission channels. The data were compiled in ENDF-6 format, and released in 1999 as a special purpose file of JENDL family, namely, JENDL Fusion File 99. (author)

  3. 76 FR 48836 - Combined Notice of Filings

    Science.gov (United States)

    2011-08-09

    ... Line Company, LLC submits tariff filing per 154.403: S-2 Tracker Filing to be effective 8/1/2011. Filed...-000. Applicants: Cimarron River Pipeline, LLC. Description: 2011 Annual Report of Cash Out Activity...

  4. Introduction to Hadoop Distributed File System

    Directory of Open Access Journals (Sweden)

    Vaibhav Gopal korat

    2012-04-01

    Full Text Available HDFS is a distributed file system designed to hold very large amounts of data (terabytes or even petabytes, and provide high-throughput access to this information. Files are stored in a redundant fashion across multiple machines to ensure their durability to failure and high availability to very parallel applications. This paper includes the step by step introduction to the file system to distributed file system and to the Hadoop Distributed File System. Section I introduces What is file System, Need of File System, Conventional File System, its advantages, Need of Distributed File System, What is Distributed File System and Benefits of Distributed File System. Also the analysis of large dataset and comparison of mapreducce with RDBMS, HPC and Grid Computing communities have been doing large-scale data processing for years. Sections II introduce the concept of Hadoop Distributed File System. Lastly section III contains Conclusion followed with the References.

  5. 78 FR 76293 - Combined Notice of Filings

    Science.gov (United States)

    2013-12-17

    ...: RP14-274-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission, Inc. submits tariff filing per 154.204: NGO Transmission, Inc.--Negotiated Rate Filing to be effective 1/1/2014. Filed...

  6. 75 FR 47296 - Combined Notice of Filings

    Science.gov (United States)

    2010-08-05

    ... Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.203: Tres Palacios Gas Storage LLC, Baseline Tariff Filing to be effective 7/28/2010. Filed Date:...

  7. Earnings Public-Use File, 2006

    Data.gov (United States)

    Social Security Administration — Social Security Administration released Earnings Public-Use File (EPUF) for 2006. File contains earnings information for individuals drawn from a systematic random...

  8. Portable File Format (PFF) specifications

    Energy Technology Data Exchange (ETDEWEB)

    Dolan, Daniel H. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-02-01

    Created at Sandia National Laboratories, the Portable File Format (PFF) allows binary data transfer across computer platforms. Although this capability is supported by many other formats, PFF files are still in use at Sandia, particularly in pulsed power research. This report provides detailed PFF specifications for accessing data without relying on legacy code.

  9. The School Library Vertical File.

    Science.gov (United States)

    Smallwood, Carol

    1990-01-01

    Discusses the maintenance of vertical files in the school library. Topics covered include circulation, weeding, using materials for special displays, acquiring materials, policies on advertising and controversial issues, cross-references, subject headings, introducing students to vertical files, beginning a collection, and preservation. (MES)

  10. Mixed-Media File Systems

    NARCIS (Netherlands)

    Bosch, Hendrikus Gerardus Petrus

    1999-01-01

    This thesis addresses the problem of implementing mixed-media storage systems. In this work a mixed-media file system is defined to be a system that stores both conventional (best-effort) file data and real-time continuous-media data. Continuous-media data is usually bulky, and servers storing and r

  11. XML Files: MedlinePlus

    Science.gov (United States)

    ... Health Terms: Vitamins XML (9 K) XML Schema Definition (XSD, 2 K) Health Services Vocabulary This file contains information on all of ... Local Web site. The file contains service terms definitions and see references service types for each service term The National Library ...

  12. The DNA Files

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-06-09

    The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the second quarter of 1998. It includes the spring/summer 1998 newsletter, the winter 1998 newsletter, the program clock, and the latest flyer.

  13. 38 CFR 19.51 - Officials authorized to file administrative appeals and time limits for filing.

    Science.gov (United States)

    2010-07-01

    ... file administrative appeals and time limits for filing. 19.51 Section 19.51 Pensions, Bonuses, and... Administrative Appeals § 19.51 Officials authorized to file administrative appeals and time limits for filing... to file administrative appeals within specified time limits, as follows: (a) Central...

  14. 78 FR 27372 - Combined Notice of Filings

    Science.gov (United States)

    2013-05-10

    ... 2013 Filing to be effective 8/ 1/2013. Filed Date: 5/1/13. Accession Number: 20130501-5062. Comments Due: 5 p.m. ET 5/13/13. Docket Numbers: RP13-863-000. Applicants: Big Sandy Pipeline, LLC. Description: FOSA Modifications May 2013 Filing to be effective 8/ 1/2013. Filed Date: 5/1/13. Accession...

  15. 77 FR 20810 - Combined Notice of Filings

    Science.gov (United States)

    2012-04-06

    ...: Big Sandy-EQT Capacity Release Waiver Request to be effective N/A. Filed Date: 3/30/12. Accession... and Sales Report of Rockies Express Pipeline LLC. Filed Date: 3/30/12. Accession Number: 20120330-5140.... Description: QEP 37657-15 Amendment to Negotiated Rate Agreement filing to be effective 4/1/2012. Filed...

  16. 48 CFR 1404.802 - Contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract files. 1404.802 Section 1404.802 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.802 Contract files. In addition to the requirements in FAR 4.802, files...

  17. 50 CFR 20.155 - Public file.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public file. 20.155 Section 20.155... § 20.155 Public file. (a) Establishment. A public file will be established for each rulemaking to which.... 552, a public file established pursuant to paragraph (a) of this section will contain: (1) The...

  18. 77 FR 56197 - Combined Notice of Filings

    Science.gov (United States)

    2012-09-12

    ... received the following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP11-2473-000. Applicants: Gulf South Pipeline Company, LP. Description: Annual Cash-In/Cash-Out Filing--2012. Filed Date: 8/31/12. Accession Number: 20120831-5044. Comments Due: 5 p.m. ET...

  19. Konaklama İşletmelerinde Kat Hizmetleri Çalışanlarının Hizmet İçi Eğitim Kalite Algısı ile Motivasyon Düzeyleri Arasındaki İlişki: İstanbul Bölgesinde Bir Araştırma(Relationship Between In-Service Training Quality Perception and Motivation Factors of Hospitality Businesses Housekeeping Employees: A Research In Istanbul Area)

    National Research Council Canada - National Science Library

    Haluk TANRIVERDİ; Mehmet Altuğ ŞAHİN

    2014-01-01

    .... Surveys were conducted on 110 housekeeping employees of two hospitality businesses’; scales of in-service training quality perception, intrinsic job motivational factors and extrinsic job motivational factors are being...

  20. PC Inspector File Recovery

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    PC Inspector File Recovery是一个数据修复软件,它支持Fat12/16/32和NTFS文件系统。3.x的新特点:1,即使在引导区或FAT被删除或损坏的情况下,也能自动寻找到驱动器(对NTFS系统不具有该功能)。2.按照原始的时间和日期重新恢复文件。3.支持恢复文件的储存及网络驱。

  1. PKA spectrum file

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, M. [Toshiba Corp., Kawasaki, Kanagawa (Japan). Nuclear Engineering Lab.

    1997-03-01

    In the Japanese Nuclear Data Committee, the PKA/KERMA file containing PKA spectra, KERMA factors and DPA cross sections in the energy range between 10{sup -5} eV and 50 MeV is being prepared from the evaluated nuclear data. The processing code ESPERANT was developed to calculate quantities of PKA, KERMA and DPA from evaluated nuclear data for medium and heavy elements by using the effective single particle emission approximation (ESPEA). For light elements, the PKA spectra are evaluated by the SCINFUL/DDX and EXIFON codes, simultaneously with other neutron cross sections. The DPA cross sections due to charged particle emitted from light elements are evaluated for high neutron energy above 20 MeV. (author)

  2. Physician Compare National Downloadable File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Physician Compare National Downloadable File is organized at the individual eligible professional level; each line is unique at the professional/enrollment...

  3. Registering Researchers in Authority Files

    NARCIS (Netherlands)

    Altman, M.; Conlon, M.; Cristan, A.L.; Dawson, L.; Dunham, J.; Hickey, T.; Hook, D.; Horstmann, W.; MacEwan, A.; Schreur, P.; Smart, L.; Smith-Yoshimura, K.; Wacker, M.; Woutersen, S.

    2014-01-01

    Registering researchers in some type of authority file or identifier system has become more compelling as both institutions and researchers recognize the need to compile their scholarly output. The report presents functional requirements and recommendations for six stakeholders: researchers, funders

  4. Cytoscape file of chemical networks

    Data.gov (United States)

    U.S. Environmental Protection Agency — The maximum connectivity scores of pairwise chemical conditions summarized from Cmap results in a file with Cytoscape format (http://www.cytoscape.org/). The figures...

  5. Denominator File - Limited Data Set

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Denominator File combines Medicare beneficiary entitlement status information from administrative enrollment records with third-party payer information and GHP...

  6. Curved canals: Ancestral files revisited

    Directory of Open Access Journals (Sweden)

    Jain Nidhi

    2008-01-01

    Full Text Available The aim of this article is to provide an insight into different techniques of cleaning and shaping of curved root canals with hand instruments. Although a plethora of root canal instruments like ProFile, ProTaper, LightSpeed ® etc dominate the current scenario, the inexpensive conventional root canal hand files such as K-files and flexible files can be used to get optimum results when handled meticulously. Special emphasis has been put on the modifications in biomechanical canal preparation in a variety of curved canal cases. This article compiles a series of clinical cases of root canals with curvatures in the middle and apical third and with S-shaped curvatures that were successfully completed by employing only conventional root canal hand instruments.

  7. 柠檬明串珠菌及相近种部分持家基因的系统发育分析%Phylogenetic analysis of closely related Leuconostoc citreum species based on partial housekeeping genes

    Institute of Scientific and Technical Information of China (English)

    吕嫱; 陈明; 徐海燕; 宋宇琴; 孙志宏; 丹彤; 孙天松

    2013-01-01

    [Objective] Using the 16S rRNA,dnaA,murC and pyrG gene sequences,we identified the phylogenetic relationship among closely related Leuconostoc citreum species.[Methods] Seven Leu.citreum strains originally isolated from sourdough were characterized by PCR methods to amplify the dnaA,murC and pyrG gene sequences,which were determined to assess the suitability as phylogenetic markers.Then,we estimated the genetic distance and constructed the phylogenetic trees including 16S rRNA and above mentioned three housekeeping genes combining with published corresponding sequences.[Results] By comparing the phylogenetic trees,the topology of three housekeeping genes trees were consistent with that of 16S rRNA gene.The homology of closely related Leu.citreum species among dnaA,murC,pyrG and 16S rRNA gene sequences were different,ranged from75.5% to 97.2%,50.2% to 99.7%,65.0% to 99.8% and 98.5% 100%,respectively.[Conclusion] The phylogenetic relationship of three housekeeping genes sequences were highly consistent with the results of 16S rRNA gene sequence,while the genetic distance of these housekeeping genes were extremely high than 16S rRNA gene.Consequently,the dnaA,murC and pyrG gene are suitable for classification and identification closely related Leu.citreum species.%[目的]利用16S rRNA、dnaA、murC和pyrG基因分子标记研究Leuconostoc citreum(Leu.citreum)及相近种间的种系发育关系,并比较这些基因序列对Leu.citreum及相近种的区分能力.[方法]以分离自酸面团中的7株Leu.citreum为研究对象,以dnaA、murC和pyrG基因片段为标记,通过PCR扩增、测序,结合已公布的近缘种及亚种相应序列,计算遗传距离,构建系统发育树,并与16S rRNA基因进行比较.[结果]研究发现Leu.citreum及相近种间的dnaA、murC和pyrG基因构建的系统发育树拓扑结构与16S rRNA基因基本一致,区别在于相似性的不同,其分别为75.5%-97.2%、50.2%-99.7%、65.0%-99.8%和98

  8. Search the SEC website for the latest EDGAR filings

    Data.gov (United States)

    Securities and Exchange Commission — This listing contains the most recent filings for the current official filing date (including filings made after the 5:30pm deadline on the previous filing day)....

  9. Dynamic Metadata Management in Semantic File Systems

    Directory of Open Access Journals (Sweden)

    T. Anand

    2015-03-01

    Full Text Available The progression in data capacity and difficulty inflicts great challenges for file systems. To address these contests, an inventive namespace management scheme is in distracted need to deliver both the ease and competence of data access. For scalability, each server makes only local, autonomous decisions about relocation for load balancing. Associative access is provided by a traditional extension to present tree-structured file system conventions, and by protocols that are intended specifically for content based access.Rapid attributebased accesstofile system contents is fulfilled by instinctive extraction and indexing of key properties of file system objects. The programmed indexing of files and calendars is called “semantic” because user programmable transducers use data about the semantics of efficient file system objects to extract the properties for indexing. Tentative results from a semantic file system execution support the thesis that semantic file systems present a more active storage abstraction than do traditional tree planned file systems for data sharing and command level programming. Semantic file system is executed as a middleware in predictable file systems and works orthogonally with categorized directory trees. The semantic relationships and file groups recognized in file systems can also be used to facilitate file prefetching among other system-level optimizations. Allencompassing trace-driven experiments on our sample implementation validate the efficiency and competence.

  10. 12 CFR 1780.9 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing of papers. 1780.9 Section 1780.9 Banks... papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed... Director or the presiding officer. All papers filed by electronic media shall also concurrently be filed...

  11. OTS: a program for converting Noldus Observer data files to SDIS files.

    Science.gov (United States)

    Bakeman, R; Quera, V

    2000-02-01

    A program for converting Noldus Observer data files (ODF) to sequential data interchange standard (SDIS) files is described. Observer users who convert their data files can then take advantage of various flexible and powerful data modification and computational procedures available in the Generalized Sequential Querier, a program that assumes SDIS-formatted files.

  12. Did accelerated filing requirements and SOX Section 404 affect the timeliness of 10-K filings?

    NARCIS (Netherlands)

    J. Impink (Joost); M. Lubberink (Martien); B. van Praag (Bart); D. Veenman (David)

    2012-01-01

    textabstractThis paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing. While Section 404 compliance does not affect filing tim

  13. Did Accelerated Filing Requirements and Sox Section 404 Affect the Timeliness of 10-K Filings?

    NARCIS (Netherlands)

    Impink, J.; Lubberink, M.; van Praag, B.; Veenman, D.

    2012-01-01

    This paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing. While Section 404 compliance does not affect filing timeliness for

  14. Did accelerated filing requirements and SOX section 404 affect the timeliness of 10-K filings?

    NARCIS (Netherlands)

    Impink, J.; Lubberink, M.; van Praag, B.; Veenman, D.

    2010-01-01

    This paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing; that Section 404 compliance does not affect filing timeliness for f

  15. Cut-and-paste file-systems: integrating simulators and file-systems

    NARCIS (Netherlands)

    Bosch, Peter; Mullender, Sape J.

    1996-01-01

    We have implemented an integrated and configurable file system called the PFS and a trace-driven file-system simulator called Patsy. Patsy is used for off-line analysis of file-system algorithms, PFS is used for on-line file-system data storage. Algorithms are first analyzed in Patsy and when we are

  16. Cut-and-paste file-systems: integrating simulators and file systems

    NARCIS (Netherlands)

    Bosch, Peter; Mullender, Sape J.

    1995-01-01

    We have implemented an integrated and configurable file system called the Pegasus filesystem (PFS) and a trace-driven file-system simulator called Patsy. Patsy is used for off-line analysis of file-systemalgorithms, PFS is used for on-line file-systemdata storage. Algorithms are first analyzed in Pa

  17. 49 CFR 564.5 - Information filing; agency processing of filings.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Information filing; agency processing of filings... HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION REPLACEABLE LIGHT SOURCE INFORMATION (Eff. until 12-01-12) § 564.5 Information filing; agency processing of filings. (a) Each...

  18. WATSTORE Stream Flow Basin Characteristics File

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Stream Flow Basin Characteristics file contains information about the drainage basins of selected USGS gaging stations. Data elements of this file were converted...

  19. 77 FR 13604 - Notice of Agreements Filed

    Science.gov (United States)

    2012-03-07

    ...-Linie GmbH & Cie. KG. Filing Party: Wade S. Hooker, Esquire; 211 Central Park W.; New York, NY 10024.... Parties: Great White Fleet (US) Ltd. and Agriculture Investment Export, Inc. Filing Party: Wade S. Hooker...

  20. 77 FR 506 - Notice of Agreements Filed

    Science.gov (United States)

    2012-01-05

    ... Express, LLC. Filing Party: Wade S. Hooker, Esq.; 211 Central Park W; New York, NY 10024. Synopsis: The... Interoceanica, S.A and Hamburg Sudamerikanische Dampfschiffahrts-Gesellschaft KG. Filing Party: Wade S. Hooker...

  1. 78 FR 76624 - Notice of Agreements Filed

    Science.gov (United States)

    2013-12-18

    ... & Logistic GmbH & Co. KG; Industrial Maritime Carriers LLC; Seaboard Marine, Ltd. Filing Party: Wade S...H & Cie. KG. Filing Party: Wade S. Hooker, Esq.; 211 Central Park W; New York, NY 10024. Synopsis...

  2. 77 FR 60433 - Notice of Agreements Filed

    Science.gov (United States)

    2012-10-03

    ... Express, Inc. and Great White Fleet Liner Services Ltd. Filing Party: Wade S. Hooker, Esq., 211 Central... White Fleet Liner Services Ltd. and Crowley Latin American Services, LLC. Filing Party: Wade S. Hooker...

  3. 77 FR 40350 - Combined Notice of Filings

    Science.gov (United States)

    2012-07-09

    ... Due: 5 p.m. ET 7/11/12. Docket Numbers: RP12-833-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission--Filing to Update Contact Information to be effective 8/1/2012. Filed Date:...

  4. 75 FR 39229 - Combined Notice of Filings

    Science.gov (United States)

    2010-07-08

    ..., July 06, 2010. Docket Numbers: RP10-875-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission, Inc. submits tariff filing per 154.203: NGO Transmission--Baseline eTariff Filing to be...

  5. Chronic Condition Public Use File (PUF)

    Data.gov (United States)

    U.S. Department of Health & Human Services — This release contains the Chronic Conditions Public Use Files (PUF) with information from Medicare claims. The CMS Chronic Conditions PUF is an aggregated file in...

  6. Fail-over file transfer process

    Science.gov (United States)

    Semancik, Susan K. (Inventor); Conger, Annette M. (Inventor)

    2005-01-01

    The present invention provides a fail-over file transfer process to handle data file transfer when the transfer is unsuccessful in order to avoid unnecessary network congestion and enhance reliability in an automated data file transfer system. If a file cannot be delivered after attempting to send the file to a receiver up to a preset number of times, and the receiver has indicated the availability of other backup receiving locations, then the file delivery is automatically attempted to one of the backup receiving locations up to the preset number of times. Failure of the file transfer to one of the backup receiving locations results in a failure notification being sent to the receiver, and the receiver may retrieve the file from the location indicated in the failure notification when ready.

  7. 76 FR 4101 - Combined Notice of Filings

    Science.gov (United States)

    2011-01-24

    ...-1001-001. Applicants: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.203: Tres Palacios Gas Storage--Address Change to be effective 2/7/2011. Filed...

  8. 76 FR 54756 - Combined Notice of Filings

    Science.gov (United States)

    2011-09-02

    ... Numbers: RP11-2436-000. Applicants: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.402: Tres Palacios Gas Storage LLC-ACA Tariff Filing to be...

  9. source files for manuscript in tex format

    Data.gov (United States)

    U.S. Environmental Protection Agency — Source tex files used to create the manuscript including original figure files and raw data used in tables and inline text. This dataset is associated with the...

  10. Structure and Application pf WAV File

    Institute of Scientific and Technical Information of China (English)

    Guo,Xingji

    2005-01-01

    As regards audio digitization, the researcher introduced several computer process means of audio information, and then, presented application patterns based on WAV file after analyzing thoroughly the structure of the widely used WAV File in computer application field.

  11. Physician Fee Schedule Carrier Specific Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Centers for Medicare and Medicaid Services (CMS) has condensed all 56 Physician Fee Schedule (PFS) carrier specific pricing files into one zip file. It is...

  12. Fermi FT2 Spacecraft Pointing Files

    Data.gov (United States)

    National Aeronautics and Space Administration — This utility permits you to download the most current version of the spacecraft (FT2) file predicting the LAT's pointing for a given mission week. The FT2 file is a...

  13. 12 CFR 303.221 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall...

  14. Ambulance Reasonable Charge Public Use Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Ambulance Reasonable Charge public use files for calendar years (CY) 2003 through 2005 are located in the Downloads section below. These public use files are...

  15. Physician-Supplier Procedure Summary Master File

    Data.gov (United States)

    U.S. Department of Health & Human Services — This file is a 100 percent summary of all Part B Carrier and DMERC Claims processed through the Common Working File and stored in the National Claims History...

  16. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Science.gov (United States)

    2010-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If...

  17. System for Multicast File Transfer

    Directory of Open Access Journals (Sweden)

    Dorin Custura

    2012-03-01

    Full Text Available The distribution of big files over the network from a single source to a large number of recipients is not efficient by using standard client-server or even peer-to peer file transfer protocols.  Thus, the transfer of a hierarchy of big files to multiple destinations can be optimized in terms of bandwidth usage and data storage reads by using multicast networking. In order to achieve that, a simple application layer protocol can be imagined. It uses multicast UDP as transport and it provides a mechanism for data ordering and retransmission. Some security problems are also considered in this protocol, because at this time the Internet standards supporting multicast security are still in the development stage.

  18. 78 FR 14779 - Combined Notice of Filings

    Science.gov (United States)

    2013-03-07

    ... calendar year 2012 activity Filed Date: 2/28/13 Accession Number: 20130228-5084 Comments Due: 5 p.m. ET 3... Applicants: Tennessee Gas Pipeline Company, L.L.C. Description: Fuel Tracker 2013 to be effective 4/1/2013... Tracker Filing effective April 1, 2013 to be effective 4/1/2013 Filed Date: 3/1/13 Accession...

  19. 47 CFR 1.1405 - File numbers.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false File numbers. 1.1405 Section 1.1405... Procedures § 1.1405 File numbers. Each complaint which appears to be essentially complete under § 1.1404 will be accepted and assigned a file number. Such assignment is for administrative purposes only and...

  20. 76 FR 45788 - Combined Notice of Filings

    Science.gov (United States)

    2011-08-01

    ...: Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011. Accession Number...: ETNG Modifications for Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011... Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011. Accession...

  1. 48 CFR 204.802 - Contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contract files. 204.802 Section 204.802 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE GENERAL ADMINISTRATIVE MATTERS Contract Files 204.802 Contract files. Official...

  2. Glue: Linked data visualizations across multiple files

    Science.gov (United States)

    Beaumont, Chris; Robitaille, Thomas; Borkin, Michelle

    2014-02-01

    Glue, written in Python, links visualizations of scientific datasets across many files, allowing for interactive, linked statistical graphics of multiple files. It supports many file formats including common image formats (jpg, tiff, png), ASCII tables, astronomical image and table formats (FITS, VOT, IPAC), and HDF5. Custom data loaders can also be easily added. Glue is highly scriptable and extendable.

  3. 76 FR 53674 - Combined Notice of Filings

    Science.gov (United States)

    2011-08-29

    .... Applicants: Sabine Pipe Line LLC. Description: Sabine Pipe Line LLC submits tariff filing per 154.205(b): Sabine Pipe Line LLC Amendment to 2011 ACA Filing to be effective 10/1/2011. Filed Date: 08/18/2011... Numbers: RP11-2387-002. Applicants: Sabine Pipe Line LLC. Description: Sabine Pipe Line LLC submits...

  4. 10 CFR 501.35 - Public file.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Public file. 501.35 Section 501.35 Energy DEPARTMENT OF... Hearings and Conferences During Administrative Proceedings § 501.35 Public file. (a) Contents. The public file shall consist of the rule, order, or petition, with supporting data and supplemental...

  5. 40 CFR 720.95 - Public file.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Public file. 720.95 Section 720.95... PREMANUFACTURE NOTIFICATION Confidentiality and Public Access to Information § 720.95 Public file. All..., will become part of the public file for that notice, unless such materials are claimed confidential....

  6. 40 CFR 725.95 - Public file.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Public file. 725.95 Section 725.95... Public file. All information submitted, including any health and safety study of a microorganism and other supporting documentation, will become part of the public file for that submission, unless...

  7. 37 CFR 1.251 - Unlocatable file.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Unlocatable file. 1.251 Section 1.251 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... § 1.251 Unlocatable file. (a) In the event that the Office cannot locate the file of an...

  8. 47 CFR 73.1943 - Political file.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Political file. 73.1943 Section 73.1943... Rules Applicable to All Broadcast Stations § 73.1943 Political file. (a) Every licensee shall keep and permit public inspection of a complete and orderly record (political file) of all requests for...

  9. 47 CFR 76.1701 - Political file.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Political file. 76.1701 Section 76.1701... CABLE TELEVISION SERVICE Documents to be Maintained for Inspection § 76.1701 Political file. (a) Every... (political file) of all requests for cablecast time made by or on behalf of a candidate for public...

  10. 78 FR 41049 - Combined Notice of Filings

    Science.gov (United States)

    2013-07-09

    ... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP13-995-000. Applicants: Natural Gas Pipeline Company of America. Description: Natural Gas... effective 7/ 1/2013. Filed Date: 06/25/2013. Accession Number: 20130625-5059. Comment Date: 5:00...

  11. 78 FR 59663 - Combined Notice of Filings

    Science.gov (United States)

    2013-09-27

    ... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP09-427-000. Applicants: Southern Natural Gas Company, L.L.C. Description: Report/Form of Southern Natural Gas Company, L.L.C. Filed Date: 9/3/13. Accession Number: 20130903-5037. Comments Due: 5...

  12. Creating Graphics Files for SGML Documents.

    Science.gov (United States)

    Lunemann, Rhonda S.

    1995-01-01

    Suggests that, when creating graphics files for documents in a Standard Generalized Markup Language (SGML) environment, technical writers should consider the differences between bitmapped and vector file formats; be familiar with available file formats; determine which graphics format best meets the needs; and incorporate the graphic into the SGML…

  13. 11 CFR 9006.2 - Filing dates.

    Science.gov (United States)

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Filing dates. 9006.2 Section 9006.2 Federal Elections FEDERAL ELECTION COMMISSION PRESIDENTIAL ELECTION CAMPAIGN FUND: GENERAL ELECTION FINANCING REPORTS AND RECORDKEEPING § 9006.2 Filing dates. The reports required to be filed under 11 CFR...

  14. FileMaker Pro 9

    CERN Document Server

    Coffey, Geoff

    2007-01-01

    FileMaker Pro 9: The Missing Manual is the clear, thorough and accessible guide to the latest version of this popular desktop database program. FileMaker Pro lets you do almost anything with the information you give it. You can print corporate reports, plan your retirement, or run a small country -- if you know what you're doing. This book helps non-technical folks like you get in, get your database built, and get the results you need. Pronto.The new edition gives novices and experienced users the scoop on versions 8.5 and 9. It offers complete coverage of timesaving new features such as the Q

  15. The Crystallographic Information File (CIF

    Directory of Open Access Journals (Sweden)

    I D Brown

    2006-11-01

    Full Text Available The Crystallographic Information File (CIF, owned by the International Union of Crystallography, is a file structure based on tag-value ASCII pairs with tags defined in machine-readable dictionaries. The crystallographic community publishes and archives large quantities of numeric information generated by crystal structure determinations, and CIF's acceptance was assured by its adoption as the submission format for Acta Crystallographica and by the obvious needs of the community. CIF's strength lies in its dictionaries, which define most of the concepts of crystallography; its weakness is the difficulty of writing software that exploits its full potential.

  16. Protecting your files on the AFS file system

    CERN Multimedia

    2011-01-01

    The Andrew File System is a world-wide distributed file system linking hundreds of universities and organizations, including CERN. Files can be accessed from anywhere, via dedicated AFS client programs or via web interfaces that export the file contents on the web. Due to the ease of access to AFS it is of utmost importance to properly protect access to sensitive data in AFS. As the use of AFS access control mechanisms is not obvious to all users, passwords, private SSH keys or certificates have been exposed in the past. In one specific instance, this also led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed in April 2010 to apply more stringent folder protections to all AFS user folders. The goal of this data protection policy is to assist users in...

  17. Adolescent Fertility: National File [Machine-Readable Data File].

    Science.gov (United States)

    Moore, Kristin A.; And Others

    This computer file contains recent cross sectional data on adolescent fertility in the United States for 1960, 1965, 1970, 1975 and 1980-85. The following variables are included: (1) births; (2) birth rates; (3) abortions; (4) non-marital childbearing; (5) infant mortality; and (6) low birth weight. Data for both teenagers and women aged 20-24 are…

  18. Protecting your files on the DFS file system

    CERN Multimedia

    Computer Security Team

    2011-01-01

    The Windows Distributed File System (DFS) hosts user directories for all NICE users plus many more data.    Files can be accessed from anywhere, via a dedicated web portal (http://cern.ch/dfs). Due to the ease of access to DFS with in CERN it is of utmost importance to properly protect access to sensitive data. As the use of DFS access control mechanisms is not obvious to all users, passwords, certificates or sensitive files might get exposed. At least this happened in past to the Andrews File System (AFS) - the Linux equivalent to DFS) - and led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed recently to apply more stringent protections to all DFS user folders. The goal of this data protection policy is to assist users in pro...

  19. The validation of housekeeping genes as a reference in quantitative Real Time PCR analysis: application in the milk somatic cells and frozen whole blood of goats infected with caprine arthritis encephalitis virus.

    Science.gov (United States)

    Jarczak, Justyna; Kaba, Jarosław; Bagnicka, Emilia

    2014-10-10

    The validation of housekeeping genes (HKGs) for normalization of RNA expression in Real-Time PCR is crucial to obtain the most reliable results. There is limited information on reference genes used in the study of gene expression in milk somatic cells and the frozen whole blood of goats. Thus, the aim of this study was to propose the most stable housekeeping genes that can be used as a reference in Real-Time PCR analysis of milk somatic cells and whole blood of goats infected with caprine arthritis encephalitis virus (CAEV). Animals were divided into two groups: non-infected (N=13) and infected with CAEV (N=13). Biological material (milk somatic cells and whole blood) was collected 4 times during the lactation period (7, 30, 100 and 240days post-partum). The expression levels of candidate reference genes were analyzed using geNorm and NormFinder software. The stability of candidates for reference gene expression was analyzed for CAEV-free (control) and CAEV-infected groups, and also for both groups together (combined group). The stability of expression of β-actin (ACTB), glyceraldehyde-3P-dehydrogenase (GAPDH), cyclophilin A (PPIA), RNA18S1, ubiquilin (UBQLN1) and ribosomal protein large subunit P0 (RPLP0) was determined in milk somatic cells, while ACTB, PPIA, RPLP0, succinate dehydrogenase complex subunit A (SDHA), zeta polypeptide (YWHAZ), battenin (CLN3), eukaryotic translation initiation factor 3K (EIF3K) and TATA box-binding protein (TBP) were measured in frozen whole blood of goats. PPIA and RPLP0 were considered as the most suitable internal controls as they were stably expressed in milk somatic cells regardless of disease status, according to NormFinder software. Furthermore, geNorm results indicated the expression of PPIA/RPLP0 genes as the best combination under these experimental conditions. The results of frozen whole blood analysis using NormFinder software revealed that the most stable reference gene in control, CAEV-infected and combined groups is

  20. Registering Researchers in Authority Files

    Science.gov (United States)

    Smith-Yoshimura, Karen; Altman, Micah; Conlon, Michael; Cristán, Ana Lupe; Dawson, Laura; Dunham, Joanne; Hickey, Thom; Hook, Daniel; Horstmann, Wolfram; MacEwan, Andrew; Schreur, Philip; Smart, Laura; Wacker, Melanie; Woutersen, Saskia

    2014-01-01

    Written by OCLC Research Program Officer Karen Smith-Yoshimura and the 13 members of the Registering Researchers in Authority Files Task Group comprised of specialists from the US, UK, and the Netherlands, this report summarizes their research into approaches to providing authoritative researcher identifiers. Registering researchers in some type…

  1. Analyzing data files in SWAN

    CERN Document Server

    Gajam, Niharika

    2016-01-01

    Traditionally analyzing data happens via batch-processing and interactive work on the terminal. The project aims to provide another way of analyzing data files: A cloud-based approach. It aims to make it a productive and interactive environment through the combination of FCC and SWAN software.

  2. When Husbands File for Divorce.

    Science.gov (United States)

    Dixon, Ruth B.; Weitzman, Lenore J.

    1982-01-01

    Explores the effects of no-fault divorce laws on the motivations of husbands who file for divorce. Tested a resource model and a normative model on divorce records of couples receiving final decrees in California. Husbands' motives were more clearly revealed in their requests for divorce settlements, particularly child custody. (Author/RC)

  3. A Metadata-Rich File System

    Energy Technology Data Exchange (ETDEWEB)

    Ames, S; Gokhale, M B; Maltzahn, C

    2009-01-07

    Despite continual improvements in the performance and reliability of large scale file systems, the management of file system metadata has changed little in the past decade. The mismatch between the size and complexity of large scale data stores and their ability to organize and query their metadata has led to a de facto standard in which raw data is stored in traditional file systems, while related, application-specific metadata is stored in relational databases. This separation of data and metadata requires considerable effort to maintain consistency and can result in complex, slow, and inflexible system operation. To address these problems, we have developed the Quasar File System (QFS), a metadata-rich file system in which files, metadata, and file relationships are all first class objects. In contrast to hierarchical file systems and relational databases, QFS defines a graph data model composed of files and their relationships. QFS includes Quasar, an XPATH-extended query language for searching the file system. Results from our QFS prototype show the effectiveness of this approach. Compared to the defacto standard, the QFS prototype shows superior ingest performance and comparable query performance on user metadata-intensive operations and superior performance on normal file metadata operations.

  4. Semantic-Discovery of Construction Project Files

    Institute of Scientific and Technical Information of China (English)

    PAN Jiayi; Chimay J. Anumba

    2008-01-01

    The dynamics of a construction project generates a huge amount of information for processing.Most project information is recorded in digital files and managed in a decentralized way. Current document management tools do not consider the semantic relationships between files and then cannot discover files based on their content or ensure information consistency across various information repositories. This paper presents a semantic-discovery method for accessing project information across repositories. By adopting semantic web technologies, including extensible markup language (XML), ontology, and logic rules, the se-mantic-discovery tool can access project files by their properties and relations. The method has been ap-plied to searching for files using documents from a real project. The results show that the semantic content of a file can be used to ensure that only related files are retrieved.

  5. Desocupación, trabajo doméstico y desigualdad: una mirada desde el uso del tiempo en Rosario, Argentina Unemployment, housekeeping and inequality: a reading from a time use survey in Rosario, Argentina

    Directory of Open Access Journals (Sweden)

    María Andrea Delfino

    2012-12-01

    Full Text Available Las transformaciones en el mercado de trabajo y en los sistemas de bienestar conllevan una fuerte tensión entre los recursos disponibles y las necesidades existentes en los hogares. Esa inadecuación entre recursos y necesidades es cubierta - en parte - con un incremento del trabajo no remunerado realizado al interior de los hogares. En este sentido, este artículo tiene por objetivo mensurar el tiempo asignado por un grupo de desocupados asistidos por el Estado al trabajo doméstico, describir el tipo de actividades básicas que componen este trabajo y caracterizar el reparto diferencial de esas actividades entre hombres y mujeres. Para alcanzar los objetivos planteados se recurrió a la metodología de uso del tiempo a través de la técnica del diario autoadministrado.The changes in the work market and in the welfare systems imply a strong tension between the available resources and the existing household needs. Such inadequacy between resources and needs is compensated -in part - by an increase in unpaid housework. In this sense, this article aims to estimate the time assigned by a group of unemployed people aided by the State to housekeeping; describe which kind of basic activities this work requires; and analyze the dissimilar division of activities between men and women. In order to attain the expected objectives, a time use survey was carried out through a self-administrated diary technique.

  6. Exploration based on robot housekeeper of smart home automation%“机器人管家”主理下的智能家庭自动化初探

    Institute of Scientific and Technical Information of China (English)

    孙少山

    2014-01-01

    Based on the analysis and understanding of existing scientific and technological achievements of the automatic con -trol, this article does the research and scientific predictions of the tendency of home automation development .Studies suggest that home automation will make progress toward integrated automation and intelligent control automation ; robot housekeeper will become an important member of smart home .Smart home automation will really push human life to be a smart era .%在分析和把握现有自动控制科技成果的基础上,对家庭自动化的发展趋势进行了研究和科学预测。研究认为,家庭自动化将向着综合自动化和智能自动化发展,“机器人管家”将成为智能家居中的核心部件和关键。智能家庭自动化将人类生活真正推向智能时代。

  7. Small file aggregation in a parallel computing system

    Science.gov (United States)

    Faibish, Sorin; Bent, John M.; Tzelnic, Percy; Grider, Gary; Zhang, Jingwang

    2014-09-02

    Techniques are provided for small file aggregation in a parallel computing system. An exemplary method for storing a plurality of files generated by a plurality of processes in a parallel computing system comprises aggregating the plurality of files into a single aggregated file; and generating metadata for the single aggregated file. The metadata comprises an offset and a length of each of the plurality of files in the single aggregated file. The metadata can be used to unpack one or more of the files from the single aggregated file.

  8. File Transfer Algorithm for Autonomous Decentralized System

    Institute of Scientific and Technical Information of China (English)

    GUI Xun; TAN Yong-dong; Qian Qing-quan

    2008-01-01

    A file transfer algorithm based on ADP (autonomous decentralized protocol) was proposed to solve the problem that the ADS (autonomous decentralized system) middleware (NeXUS/Dlink) lacks of file transfer functions for Windows. The algorithm realizes the peer-to-peer file transfer, one-to-N inquiry/multi-response file transfer and one-to-N file distribution in the same data field based on communication patterns provided by the ADP. The peer-to-peer file transfer is implemented through a peer-to-peer communication path, one-to-N inquiry/multi-response file transfer and one-to-N file distribution are implemented through multicast commtmieation. In this algorithm, a file to be transferred is named with a GUID ( global unique identification), every data packet is marked with a sequence number, and file-receiving in parallel is implemented by caching DPOs (data processing objects) and multithread technologies. The algorithm is applied in a simulation system of the decentralized control platform, and the test results and long time stable mrming prove the feasibility of the algorithm.

  9. Flat Files - JSNP | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ... Data file File name: jsnp_flat_files File URL: ftp://ftp.biosciencedbc.jp/archiv...his Database Database Description Download License Update History of This Database Site Policy | Contact Us Flat Files - JSNP | LSDB Archive ...

  10. 12 CFR 908.25 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing of papers. 908.25 Section 908.25 Banks... RULES OF PRACTICE AND PROCEDURE IN HEARINGS ON THE RECORD General Rules § 908.25 Filing of papers. (a) Filing. Any papers required to be filed shall be addressed to the presiding officer and filed with...

  11. 29 CFR 1978.102 - Filing of discrimination complaint.

    Science.gov (United States)

    2010-07-01

    ... or other adverse action; or where the discrimination is in the nature of a continuing violation. The... 29 Labor 9 2010-07-01 2010-07-01 false Filing of discrimination complaint. 1978.102 Section 1978... § 1978.102 Filing of discrimination complaint. (a) Who may file. An employee may file, or have filed...

  12. 46 CFR 531.5 - Duty to file.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Duty to file. 531.5 Section 531.5 Shipping FEDERAL... General Provisions § 531.5 Duty to file. (a) The duty under this part to file NSAs, amendments and notices.... (d) Registration—(1) Application. Authority to file or delegate the authority to file must...

  13. Study on the Career Fatigue of Junior Housekeeping Department Staff:A Study Based on Five-star Hotels in Xiamen%客房基层员工职业倦怠研究--以厦门市五星级饭店为例

    Institute of Scientific and Technical Information of China (English)

    刘永涓; 柯煌强

    2013-01-01

    At present, the research of career fatigue has been carried out in many fields, however the study of the career fatigue of Junior Staff of hotel Housekeeping Department is inadequate and the pre-vious research mainly focused on causes. Drawing upon previous research at home and abroad, based on a survey of junior housekeeping staff of eight five-star hotels in Xiamen which investigated housekeep-ing employees' reaction to fatigue from three aspects, the author concludes that: the junior employee's career fatigue main manifested as emotional drain and dehumanization, while the decline of sense of personal achievement is relatively less obvious. Hotel staff of different demographic traits exhibited great differences in the three dimensions career fatigue.%目前关于职业倦怠的研究在许多领域开展,但是,饭店行业基层员工职业倦怠的研究偏少,而且主要是集中在影响因素方面的研究。通过借鉴国内外在职业倦怠方面的研究,以厦门市8家五星级饭店客房基层员工为调查对象,考察客房基层员工在职业倦怠三个维度的反应情况,得出如下结论:饭店客房基层员工的职业倦怠主要表现在情感耗竭和去人性化两个维度,而在个人成就感降低的维度上表现不强;不同人口学特征的客房基层员工在职业倦怠的三个维度上有显著的差异。

  14. File Type Identification of File Fragments using Longest Common Subsequence (LCS)

    Science.gov (United States)

    Rahmat, R. F.; Nicholas, F.; Purnamawati, S.; Sitompul, O. S.

    2017-01-01

    Computer forensic analyst is a person in charge of investigation and evidence tracking. In certain cases, the file needed to be presented as digital evidence was deleted. It is difficult to reconstruct the file, because it often lost its header and cannot be identified while being restored. Therefore, a method is required for identifying the file type of file fragments. In this research, we propose Longest Common Subsequences that consists of three steps, namely training, testing and validation, to identify the file type from file fragments. From all testing results we can conlude that our proposed method works well and achieves 92.91% of accuracy to identify the file type of file fragment for three data types.

  15. Verifying compiled file system code

    OpenAIRE

    Mühlberg, Jan Tobias; Lüttgen, Gerald

    2011-01-01

    This article presents a case study on retrospective verification of the Linux Virtual File System (VFS), which is aimed at checking violations of API usage rules and memory properties. Since VFS maintains dynamic data structures and is written in a mixture of C and inlined assembly, modern software model checkers cannot be applied. Our case study centres around our novel automated software verification tool, the SOCA Verifier, which symbolically executes and analyses compi...

  16. Evaluated nuclear data file of Th-232

    Energy Technology Data Exchange (ETDEWEB)

    Meadows, J.; Poenitz, W.; Smith, A.; Smith, D.; Whalen, J.; Howerton, R.

    1977-09-01

    An evaluated nuclear data file for thorium is described. The file extends over the energy range 0.049 (i.e., the inelastic-scattering threshold) to 20.0 MeV and is formulated within the framework of the ENDF system. The input data base, the evaluation procedures and judgments, and ancillary experiments carried out in conjunction with the evaluation are outlined. The file includes: neutron total cross sections, neutron scattering processes, neutron radiative capture cross sections, fission cross sections, (n;2n) and (n;3n) processes, fission properties (e.g., nu-bar and delayed neutron emission) and photon production processes. Regions of uncertainty are pointed out particularly where new measured results would be of value. The file is extended to thermal energies using previously reported resonance evaluations thereby providing a complete file for neutronic calculations. Integral data tests indicated that the file was suitable for neutronic calculations in the MeV range.

  17. Creating and Viewing HTML Files with Python

    Directory of Open Access Journals (Sweden)

    William J. Turkel

    2012-07-01

    Full Text Available This lesson uses Python to create and view an HTML file. If you write programs that output HTML, you can use any browser to look at your results. This is especially convenient if your program is automatically creating hyperlinks or graphic entities like charts and diagrams. Here you will learn how to create HTML files with Python scripts, and how to use Python to automatically open an HTML file in Firefox.

  18. 76 FR 43679 - Filing via the Internet; Notice of Additional File Formats for efiling

    Science.gov (United States)

    2011-07-21

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Notice of Additional File Formats for efiling Take notice that the Commission has added to its list of acceptable file formats the...

  19. 76 FR 70651 - Fee for Filing a Patent Application Other Than by the Electronic Filing System

    Science.gov (United States)

    2011-11-15

    .... Information concerning electronic filing via EFS-Web is available from the USPTO's Patent Electronic Business... the Electronic Filing System AGENCY: United States Patent and Trademark Office, Commerce. ACTION... for a design, plant, or provisional application, that is not filed by electronic means as...

  20. 76 FR 49467 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-10

    ...: Modification of its Transmission Depreciation Rate to be effective 10/1/2011. Filed Date: 08/02/2011. Accession... submits tariff filing per 35.17(b): Amendment to the APS depreciation filing to add affidavit of Z....

  1. 76 FR 59674 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-09-27

    ... Logan Interconnect, LLC. Description: Peetz Logan Interconnect, LLC submits tariff filing per 35: Peetz Logan Interconnect, LLC OATT Compliance Filing to Attachment C to be effective 11/13/2011. Filed...

  2. City locations for all places in the TIGER files

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — City locations for all places in the TIGER files; this file was extracted from dbf files posted on the internet by the Bureau of the Census. This is basically a...

  3. 78 FR 34362 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-06-07

    ... Fortistar Methane Group Gas Recovery Systems, LLC. Filed Date: 5/23/13. Accession Number: 20130523-5138.... Description: Amendment to Application and Initial Baseline Tariff Filing to be effective 8/1/2013. Filed...

  4. 75 FR 47291 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-08-05

    ...: PPL Brunner Island, LLC. Description: PPL Brunner Island, LLC submits tariff filing per 35.12: PPL Brunner Island, LLC Baseline Filing to be effective 7/28/ 2010. Filed Date: 07/28/2010. Accession...

  5. 75 FR 80805 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-12-23

    ...: RP11-1613-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission, Inc. submits tariff filing per 154.204: NGO Transmission--Negotiated Rate Filing, to be effective 1/1/ 2011. Filed Date:...

  6. 75 FR 54862 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-09-09

    ... Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.203: Tres Palacios Gas Storage LLC--Order No. 587-U Compliance Filing to be effective N/A. Filed Date:...

  7. 75 FR 53287 - Combined Notice of Filings No. 2

    Science.gov (United States)

    2010-08-31

    .... Applicants: Sycamore Cogeneration Company. Description: Sycamore Cogeneration Company submits tariff filing per 35.12: Sycamore Cogeneration Company Baseline Filing to be effective 8/25/2010. Filed Date:...

  8. 76 FR 14964 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-18

    ..., LLC submits tariff filing per 35.17(b): Amendment to Evergreen Wind Power III, LLC MBR Application to... per 35.13(a)(2)(iii: ICT Extension Compliance Filing to be effective 11/17/ 2010. Filed Date:...

  9. 78 FR 77116 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-20

    ..., Inc. Description: Southwest Power Pool, Inc. submits tariff filing per 35.13(a)(2)(iii: KCPL and GMO....ferc.gov/docs-filing/efiling/filing-req.pdf . For other information, call (866) 208-3676 (toll free...

  10. 76 FR 17409 - Combined Notice of Filings #

    Science.gov (United States)

    2011-03-29

    ...% ownership interest in Green Mountain Power Corporation etc. Filed Date: 03/21/2011 Accession Number... Applicants: Ameren Energy Marketing Company Description: Ameren Energy Marketing Company submits tariff...

  11. Evaluated nuclear-data file for niobium

    Energy Technology Data Exchange (ETDEWEB)

    Smith, A.B.; Smith, D.L.; Howerton, R.J.

    1985-03-01

    A comprehensive evaluated nuclear-data file for elemental niobium is provided in the ENDF/B format. This file, extending over the energy range 10/sup -11/-20 MeV, is suitable for comprehensive neutronic calculations, particulary those dealing with fusion-energy systems. It also provides dosimetry information. Attention is given to the internal consistancy of the file, energy balance, and the quantitative specification of uncertainties. Comparisons are made with experimental data and previous evaluated files. The results of integral tests are described and remaining outstanding problem areas are cited. 107 refs.

  12. File caching in data intensive scientific applications

    Energy Technology Data Exchange (ETDEWEB)

    Otoo, Ekow; Rotem, Doron; Romosan, Alexandru; Seshadri, Sridhar

    2004-07-18

    We present some theoretical and experimental results of animportant caching problem that arises frequently in data intensivescientific applications. In such applications, jobs need to processseveral files simultaneously, i.e., a job can only be serviced if all itsneeded files are present in the disk cache. The set of files requested bya job is called a file-bundle. This requirement introduces the need forcache replacement algorithms based on file-bundles rather then individualfiles. We show that traditional caching algorithms such Least RecentlyUsed (LRU), and GreedyDual-Size (GDS), are not optimal in this case sincethey are not sensitive to file-bundles and may hold in the cachenon-relevant combinations of files. In this paper we propose and analyzea new cache replacement algorithm specifically adapted to deal withfile-bundles. We tested the new algorithm using a disk cache simulationmodel under a wide range of parameters such as file requestdistributions, relative cache size, file size distribution,and queuesize. In all these tests, the results show significant improvement overtraditional caching algorithms such as GDS.

  13. A Method to Defend File-Attacking

    Institute of Scientific and Technical Information of China (English)

    HE Hongjun; LUO Li; CAO Sihua; FENG Tao; PAN Li; ZOU Zhiji

    2006-01-01

    The paper points out that the deep reason why modern computer system fails to defense malware lies in that user has no right to control the access of information, and proposes an explicit authorization mechanism. Its basic idea is that user explicitly authorizes program the file set it can access, and monitor all file access operations; once program requests to access file out of the authorized file set, refuse it, and this means that the program is malicious or has design errors. Computers based on this novel mechanism can protect information from attacking reliably, and have good software and hardware compatibility. A testing system is presented to validate our theory.

  14. 78 FR 25071 - Combined Notice of Filings

    Science.gov (United States)

    2013-04-29

    ... Public Convenience and Necessity to Lease Pipeline Facilities and for Related Authorizations. Filed Date... abandon pipeline facility by transfer and amending certificated of public convenience and necessity....

  15. An Atempt to Search EMBASE File

    Science.gov (United States)

    Asakura, Hajime

    A brief structure of EMBASE file was explained from a standpoint of actual on-line and manual searching, and compared with MEDLINE file. EMBASE and MEDLINE files were atempted to search using the same key words as searching tags, although the former comprises an enormous number of synonyms and the latter is completely controlled by MeSH terms, subheadings and the like. As was expected, certain documents were found in EMBASE only, which suggested that these files filled up each other to provide a complete result.

  16. Qualidade de vida e sintomas osteomusculares em trabalhadores de higiene e limpeza hospitalar Calidad de vida y síntomas osteomusculares en trabajadores de la higiene y limpieza hospitalaria Quality of life and musculoskeletal symptoms in hospital housekeeping workers

    Directory of Open Access Journals (Sweden)

    Norton de Assumpção Martarello

    2009-06-01

    apuntados en el Cuestionario Nórdico y obtenidos con la aplicación del cuestionario genérico de evaluación de la Calidad de Vida (SF-36 se reveló significativo en los dominios Capacidad Funcional, Dolor, Estado General de Salud, Vitalidad e Salud Mental.This retrospective descriptive study, using structured validated questionnaires, analyzed 86 hospital housekeeping workers who were exposed to a huge diversity of occupational risks at a public municipal teaching and emergency hospital. The purpose of this study was to identify the quality of life aspects and musculoskeletal symptoms in hospital housekeeping workers. The results found by administering the Nordic Questionnaire confirmed that workers had problems in some part of their bodies. This finding accused musculoskeletal symptoms, mainly involving the following body parts: shoulders, upper back, neck and lower back. The difference between the groups of workers with or without musculoskeletal symptoms pointed out on the Nordic Questionnaire and obtained by applying the generic Quality of Life evaluation questionnaire (SF-36 was significant for the Functional Capacity, Pain, General Health Condition, Vitality and Mental Health domains.

  17. DATA Act File C Award Financial - Social Security

    Data.gov (United States)

    Social Security Administration — The DATA Act Information Model Schema Reporting Submission Specification File C. File C includes the agency award information from the financial accounting system at...

  18. PEMBUATAN PERANGKAT LUNAK WAVE MANIPULATOR UNTUK MEMANIPULASI FILE WAV

    Directory of Open Access Journals (Sweden)

    Ibnu Gunawan

    2005-01-01

    Full Text Available WAV File is a standard audio file used in Windows. Manipulation of WAV File can be done to obtain different output from the WAV File. These manipulation processes are made into an application that is capable to make changes on WAV File. WAVE Manipulator is an audio application used to change voice from audio files like WAV file. The objective of this project is to develop an application that can be used to change or give effect to the popular audio file known as WAV File using Borland Delphi 7. Application is designed using standard view to make it easier to use. The output of this research is a WAVE Manipulator Software that function as a tool to manipulate WAV File with the ability to choose part of sample for the manipulation, so that user can easily manipulate a WAV File. Abstract in Bahasa Indonesia : File WAV adalah file audio standar yang digunakan oleh Windows. Manipulasi file WAV bisa dilakukan untuk mendapatkan hasil suara yang berbeda-beda. Proses manipulasi file WAV tersebut akan dikemas menjadi suatu applikasi yang akan mampu melakukan perubahan-perubahan pada file WAV. WAVE Manipulator adalah nama dari suatu aplikasi audio yang akan dibuat untuk mengubah hasil suara dari file-file audio seperti file WAV. Tujuan dari pembuatan perangkat lunak ini adalah untuk membangun suatu aplikasi yang dapat digunakan untuk mengubah atau memberi efek pada file WAV yang merupakan salah satu file audio yang sangat dikenal dengan menggunakan Borland Delphi 7. Hasil dari penelitian ini adalah sebuah perangkat lunak yang bernama WAVE Manipulator yang akan berguna untuk melakukan manipulasi pada file WAV dilengkapi dengan kemampuan untuk menentukan bagian dari sample yang akan dimanipulasi dan lainnya untuk memudahkan pemakai program dalam melakukan manipulasi file WAV. Kata kunci: manipulasi, WAV, WAVE

  19. Experiences on File Systems: Which is the best file system for you?

    CERN Document Server

    Blomer, J

    2015-01-01

    The distributed file system landscape is scattered. Besides a plethora of research file systems, there is also a large number of production grade file systems with various strengths and weaknesses. The file system, as an abstraction of permanent storage, is appealing because it provides application portability and integration with legacy and third-party applications, including UNIX utilities. On the other hand, the general and simple file system interface makes it notoriously difficult for a distributed file system to perform well under a variety of different workloads. This contribution provides a taxonomy of commonly used distributed file systems and points out areas of research and development that are particularly important for high-energy physics.

  20. Analyzing Service Rates for File Transfers in Peer-to-peer File Sharing Systems

    Institute of Scientific and Technical Information of China (English)

    WANG Kai; PAN Li; LI Jian-hua

    2008-01-01

    When examining the file transfer performance in a peer-to-peer file sharing system, a fundamental problem is how to describe the service rate for a file transfer.In this paper, the problem is examined by analyzing the distribution of server-like nodes' upstream-bandwidth among their concurrent transfers.A sufficient condition for the service rate, what a receiver obtains for downloading a file, to asymptotically be uniform is presented.On the aggregate service rate for transferring a file in a system, a sufficient condition for it to asymptotically follow a Zipf distribution is presented.These asymptotic equalities are both in the mean square sense.These analyses and the sufficient conditions provide a mathematic base for modeling file transfer processes in peer-to-peer file sharing systems.

  1. X-Files opjat v stroju

    Index Scriptorium Estoniae

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  2. Vertical Files in Midlands Academic Libraries.

    Science.gov (United States)

    Lillis, John G.

    1991-01-01

    Reviews survey responses from 127 nonmedical academic libraries in Nebraska, Iowa, and Kansas regarding their vertical files (e.g., acquisitions, weeding, size, nature, collection management, frequency of use, maintenance of statistics, types of users, circulation, and security), reporting that 109 had vertical files, with most emphasizing topics…

  3. 37 CFR 401.16 - Electronic filing.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Electronic filing. 401.16... OF COMMERCE RIGHTS TO INVENTIONS MADE BY NONPROFIT ORGANIZATIONS AND SMALL BUSINESS FIRMS UNDER GOVERNMENT GRANTS, CONTRACTS, AND COOPERATIVE AGREEMENTS § 401.16 Electronic filing. Unless...

  4. New details emerge from the Einstein files

    CERN Multimedia

    Overbye, D

    2002-01-01

    For many years the FBI spied on Einstein. New details of this surveilance are emerging in "The Einstein File: J. Edgar Hoover's Secret War Against the World's Most Famous Scientist," by Fred Jerome, who sued the government with the help of the Public Citizen Litigation Group to obtain a less censored version of the file (1 page).

  5. 77 FR 76024 - Combined Notice of Filings

    Science.gov (United States)

    2012-12-26

    ... Number: 20121218-5086. Comments Due: 5 p.m. ET 12/31/12. Docket Numbers: RP13-399-000. Applicants: NGO Transmission, Inc. Description: NGO Transmission--Negotiated Rate Filing to be effective 1/1/2013. Filed...

  6. Aspects on Transfer of Aided - Design Files

    Science.gov (United States)

    Goanta, A. M.; Anghelache, D. G.

    2016-08-01

    At this stage of development of hardware and software, each company that makes design software packages has a certain type of file created and customized in time to distinguish that company from its competitors. Thus today are widely known the DWG files belonging AutoCAD, IPT / IAM belonging to Inventor, PAR / ASM of Solid Edge's, PRT from the NX and so on. Behind every type of file there is a mathematical model which is common to more types of files. A specific aspect of the computer -aided design is that all softwares are working with both individual parts and assemblies, but their approach is different in that some use the same type of file both for each part and for the whole (PRT ), while others use different types of files (IPT / IAM, PAR / ASM, etc.). Another aspect of the computer -aided design is to transfer files between different companies which use different software packages or even the same software package but in different versions. Each of these situations generates distinct issues. Thus, to solve the partial reading by a project different from the native one, transfer files of STEP and IGES type are used

  7. A progress report on progress files

    OpenAIRE

    East, Rob

    2005-01-01

    Abstract In order to facilitate personal development planning (PDP), the UK higher education sector is committed to introducing progress files. This article explores the experience of one institution in seeking to establish a system of progress files. It identifies the main practical problems in doing this, highlighting the lack of agreement on the skills that higher education students are expected to acqui...

  8. 76 FR 69260 - Combined Notice of Filings

    Science.gov (United States)

    2011-11-08

    ... Transmission, LLC. Description: ConEd 2011-11-01 Release to Infinite Energy to be effective 11/1/2011. Filed... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings November 2, 2011. Take notice that the...

  9. 78 FR 64940 - Notice of Agreements Filed

    Science.gov (United States)

    2013-10-30

    .... Agreement No.: 012230. Title: P3 Network Vessel Sharing Agreement. Parties: A.P. Moller-Maersk A/S trading under the name Maersk Line; CMA CGM S.A.; and MSC Mediterranean Shipping Company, S.A. Filing Party... Yusen Kaisha and Eukor Car Carrier Inc. ] Filing Party: Robert Shababb, Corporate Counsel, NYK...

  10. 48 CFR 4.802 - Contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Contract files. 4.802 Section 4.802 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE... locator system should be established to ensure the ability to locate promptly any contract files....

  11. 76 FR 39085 - Combined Notice of Filings

    Science.gov (United States)

    2011-07-05

    ...: Big Sandy Pipeline, LLC. Description: Big Sandy Pipeline, LLC submits tariff filing per 154.204..., July 05, 2011. ] Docket Numbers: RP11-2211-000. Applicants: Eastern Shore Natural Gas Company. Description: Eastern Shore Natural Gas Company submits tariff filing per 154.204: Form of Service Change for...

  12. 78 FR 72877 - Combined Notice of Filings

    Science.gov (United States)

    2013-12-04

    ...),: Statement of Operating Conditions Baseline to be effective 11/21/2013. Filed Date: 11/21/13. Accession...: RP14-200-000. Applicants: Southern Star Central Gas Pipeline, Inc. Description: Annual Operational Flow... Cash-Out Refund Report of Southern Star Central Gas Pipeline, Inc. Filed Date: 11/22/13. Accession...

  13. X-Files opjat v stroju

    Index Scriptorium Estoniae

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  14. Clockwise: a mixed-media file system

    NARCIS (Netherlands)

    Bosch, Peter; Jansen, Pierre G.; Mullender, Sape J.

    1999-01-01

    This paper presents Clockwise, a mixed-media file system. The primary goal of Clockwise is to provide a storage architecture that supports the storage and retrieval of best-effort and real-time file system data. Clockwise provides an abstraction called a dynamic partition that groups lists of relate

  15. Automating Your Ready-Reference File.

    Science.gov (United States)

    Thomas, Joy; Sottong, Stephen P.

    1994-01-01

    Describes the automation of the ready reference card file at the California State University, Long Beach library. Topics discusses include the use of dBase III Plus software; keyword fields for multiple access points; the source field and other fields; a menu-driven interface; and file creation and maintenance. (Contains five references.) (LRW)

  16. 40 CFR 78.4 - Filings.

    Science.gov (United States)

    2010-07-01

    ... Appeals Board or the Presiding Officer. (3) If any part of any filing is in a foreign language, it shall be accompanied by an English translation verified by the person making the translation, under oath... qualifications of the person making the translation. Translations filed of material originally produced in...

  17. 78 FR 25260 - Combined Notice of Filings

    Science.gov (United States)

    2013-04-30

    .... Docket Numbers: RP13-807-000. Applicants: Natural Gas Pipeline Company of America. Description... Due: 5 p.m. ET 5/6/13. Docket Numbers: RP13-806-001. Applicants: Portland Natural Gas Transmission... has received the following Natural Gas Pipeline Rate and Refund Report filings: Filings...

  18. 78 FR 70298 - Combined Notice of Filings

    Science.gov (United States)

    2013-11-25

    ...: 20131101-5081. Comments Due: 5 p.m. ET 11/22/13. Docket Numbers: RP13-1230-000. Applicants: El Paso Natural....m. ET 11/19/13. Docket Numbers: RP14-157-000. Applicants: Northern Natural Gas Company. Description... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings...

  19. 78 FR 50053 - Combined Notice of Filings

    Science.gov (United States)

    2013-08-16

    .... Docket Numbers: RP13-1231-000. Applicants: El Paso Natural Gas Company, L.L.C. Description: Non... Existing Proceedings Docket Numbers: RP10-1083-007. Applicants: Eastern Shore Natural Gas Company... received the following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting...

  20. 78 FR 75345 - Notice of Agreements Filed

    Science.gov (United States)

    2013-12-11

    ...: Compania Sud Americana de Vapores S.A. and Trans Global Shipping NV. Filing Party: Walter H. Lion Esq.; Mc... Agreement. Parties: Swordfish Shipping Inc. and Trans Global Shipping NV. Filing Party: Walter H. Lion, Esq... York and New Jersey. Parties: New York Shipping Association, Inc. and the International...

  1. Athos: Efficient Authentication of Outsourced File Systems

    DEFF Research Database (Denmark)

    Triandopoulos, Nikolaos; Goodrich, Michael T.; Papamanthou, Charalampos

    2008-01-01

    outsourced storage. Using light-weight cryptographic primitives and efficient data-structuring techniques, we design authentication schemes that allow a client to efficiently verify that the file system is fully consistent with the exact history of updates and queries requested by the client. In Athos, file-system......We study the problem of authenticated storage, where we wish to construct protocols that allow to outsource any complex file system to an untrusted server and yet ensure the file-system's integrity. We introduce Athos, a new, platform-independent and user-transparent architecture for authenticated...... operations are verified in time that is logarithmic in the size of the file system using optimal storage complexity-constant storage overhead at the client and asymptotically no extra overhead at the server. We provide a prototype implementation of Athos validating its performance and its authentication...

  2. Cellular Auxin Homeostasis:Gatekeeping Is Housekeeping

    Institute of Scientific and Technical Information of China (English)

    Michel Ruiz Rosquete; Elke Barbez; Jürgen Kleine-Vehn

    2012-01-01

    The phytohormone auxin is essential for plant development and contributes to nearly every aspect of the plant life cycle.The spatio-temporal distribution of auxin depends on a complex interplay between auxin metabolism and cell-to-cell auxin transport.Auxin metabolism and transport are both crucial for plant development;however,it largely remains to be seen how these processes are integrated to ensure defined cellular auxin levels or even gradients within tissues or organs.In this review,we provide a glance at very diverse topics of auxin biology,such as biosynthesis,conjugation,oxidation,and transport of auxin.This broad,but certainly superficial,overview highlights the mutual importance of auxin metabolism and transport.Moreover,it allows pinpointing how auxin metabolism and transport get integrated to jointly regulate cellular auxin homeostasis.Even though these processes have been so far only separately studied,we assume that the phytohormonal crosstalk integrates and coordinates auxin metabolism and transport.Besides the integrative power of the global hormone signaling,we additionally introduce the hypothetical concept considering auxin transport components as gatekeepers for auxin responses.

  3. Microbiological Assessment of Housekeeping Practices and ...

    African Journals Online (AJOL)

    SARAH

    2016-03-31

    Mar 31, 2016 ... operation are reflected in the present microbiological analyses. However, the TCC of effluents ... coupled with the poor status of public infrastructures ... bodies from abattoir waste constitutes significant ... E. coli. However, others may include Hafnia alvei and strains belonging to genera such as Buttiauxella,.

  4. Aging mechanisms in fruit files.

    Science.gov (United States)

    Tower, J

    1996-10-01

    Genetic analysis of Drosophila has provided evidence in support of two proposed evolutionary genetic mechanisms of aging: mutation accumulation and antagonistic pleiotropy. Both mechanisms result from the lack of natural selection acting on old organisms. Analyses of large numbers of files have revealed that mortality rates do not continue to rise with age as previously thought, but plateau at advanced ages. This phenomenon has implications both for models and for definitions of aging, and may be explained by the evolutionary theories. The physiological processes and genes most relevant to aging are being identified using Drosophila lines selected in the laboratory for postponed senescence. Oxidative stress and insufficient metabolic reserves/capacity may be particularly important factors in limiting the fruitfly lifespan. Genes which exhibit aging-related changes in expression are now being identified. Transgenic files are being used to analyze the mechanisms of such aging-related gene expression, and to test the effects of specific genes on aging and aging-related deterioration.

  5. 75 FR 81595 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-12-28

    ...: ER11-111-001. Applicants: Public Service Company of New Mexico. Description: Public Service Company of New Mexico submits tariff filing per 35: PNM-WAPA Compliance Filing to be effective 10/15/2010. Filed...: Cambria Submits Corrected App. B. Filed Date: 12/16/2010. Accession Number: 20101216-5124. Comment Date: 5...

  6. 21 CFR 314.420 - Drug master files.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Drug master files. 314.420 Section 314.420 Food... master files. (a) A drug master file is a submission of information to the Food and Drug Administration by a person (the drug master file holder) who intends it to be used for one of the following...

  7. Using Hierarchical Folders and Tags for File Management

    Science.gov (United States)

    Ma, Shanshan

    2010-01-01

    Hierarchical folders have been widely used for managing digital files. A well constructed hierarchical structure can keep files organized. A parent folder can have several subfolders and one subfolder can only reside in one parent folder. Files are stored in folders or subfolders. Files can be found by traversing a given path, going through…

  8. 12 CFR 308.10 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Filing of papers. 308.10 Section 308.10 Banks... AND PROCEDURE Uniform Rules of Practice and Procedure § 308.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding documents produced in response to a discovery request pursuant...

  9. 42 CFR 430.63 - Filing and service of papers.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Filing and service of papers. 430.63 Section 430.63... Conformity of State Medicaid Plans and Practice to Federal Requirements § 430.63 Filing and service of papers. (a) Filing. All papers in the proceedings are filed with the CMS Docket Clerk, in an original and...

  10. 12 CFR 509.10 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Filing of papers. 509.10 Section 509.10 Banks... IN ADJUDICATORY PROCEEDINGS Uniform Rules of Practice and Procedure § 509.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding documents produced in response to a discovery...

  11. 12 CFR 19.10 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Filing of papers. 19.10 Section 19.10 Banks and... Rules of Practice and Procedure § 19.10 Filing of papers. (a) Filing. Any papers required to be filed...) Delivering the papers to a reliable commercial courier service, overnight delivery service, or to the...

  12. 12 CFR 747.10 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Filing of papers. 747.10 Section 747.10 Banks... Practice and Procedure § 747.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding...) Delivering the papers to a reliable commercial courier service, overnight delivery service, or to the...

  13. 12 CFR 263.10 - Filing of papers.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Filing of papers. 263.10 Section 263.10 Banks... OF PRACTICE FOR HEARINGS Uniform Rules of Practice and Procedure § 263.10 Filing of papers. (a) Filing. Any papers required to be filed, excluding documents produced in response to a discovery...

  14. 45 CFR 1386.85 - Filing and service of papers.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Filing and service of papers. 1386.85 Section 1386... Requirements General § 1386.85 Filing and service of papers. (a) All papers in the proceedings must be filed... transcripts of testimony need be filed. (b) Copies of papers in the proceedings must be served on all...

  15. 48 CFR 1404.805 - Disposal of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Disposal of contract files. 1404.805 Section 1404.805 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.805 Disposal of contract files. Disposition of files shall...

  16. 75 FR 74034 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-30

    ...: Sunoco Power Marketing, LLC. Description: Sunoco Power Marketing, LLC submits tariff filing per 35: Sunoco Power Marketing Baseline Filing to be effective 11/18/2010. Filed Date: 11/18/2010. Accession... filing per 35.13(a)(2)(iii): Helper City Morgantown POD Construction Agreement to be effective 11/3/...

  17. 10 CFR 205.81 - What to file.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false What to file. 205.81 Section 205.81 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Interpretation § 205.81 What to file. (a) A person filing under this subpart shall file a “Request for Interpretation,” which should be clearly labeled...

  18. 46 CFR 530.5 - Duty to file.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Duty to file. 530.5 Section 530.5 Shipping FEDERAL... Provisions § 530.5 Duty to file. (a) The duty under this part to file service contracts, amendments and... conditions as the parties may agree. (c) Registration—(1) Application. Authority to file or delegate...

  19. 78 FR 79429 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-30

    ... 1000 Compliance Filing to be effective 2/17/2014. Filed Date: 12/18/13. Accession Number: 20131218-5197...: Midcontinent Independent System Operator, Inc. submits 12-16-2013 Entergy IAs Succession Filing Part 1 to be...: Midcontinent Independent System Operator, Inc. submits 12-16-2013 Entergy IAs Succession Filing Part 2 to...

  20. 76 FR 16621 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-24

    ... II Pie B, L.P., LS Power Partners II, L.P., Bridgeport Energy, LLC. Description: Application of LS..., Inc. submits tariff filing per 35: ICT Extension Compliance Filing to be effective 11/17/2010. Filed.... Description: Amendment to Application of Adagio Energy LLC. Filed Date: 03/16/2011. Accession Number:...

  1. JENDL FP decay data file 2000

    Energy Technology Data Exchange (ETDEWEB)

    Katakura, Jun-ichi [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Yoshida, Tadashi [Musashi Inst. of Tech., Tokyo (Japan); Oyamatsu, Kazuhiro [Aichi Shukutoku Univ., Nagakute, Aichi (Japan); Tachibana, Takahiro [Waseda Univ., Tokyo (Japan)

    2001-07-01

    A decay data file of fission product (FP) nuclides has been developed for the use in nuclear technology field as one of special purpose files of JENDL (Japanese Evaluated Nuclear Data Library) in the format of ENDF/B and it is called JENDL FP Decay Data File 2000. The file includes the decay data for 1229 fission product nuclides: 142 stable and 1087 unstable nuclides. The data included for a nuclide are decay modes, their Q values and branching ratios, average decay energy values of beta-rays, gamma-rays and alpha-particles and their spectral data. The primary source of the decay data is ENSDF (Evaluated Nuclear Structure Data File), which is the internationally recognized data file of nuclear structure properties. The data in ENSDF, however, cover only measured ones. The data of the short-lived nuclides needed for the application fields such as decay heat prediction are often incomplete or not measured because of their short half-lives. For such nuclides a theoretical model calculation is applied to derive the needed data such as average decay energies and spectral data. The data in JENDL FP Decay Data File 2000 have been tested by summation calculation comparing its results with measured data of decay heat values and aggregate fission product spectra of various fissioning nuclei. The comparison showed good agreement between the calculated results and the measured values. (author)

  2. 78 FR 21930 - Aquenergy Systems, Inc.; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2013-04-12

    ... Energy Regulatory Commission Aquenergy Systems, Inc.; Notice of Intent To File License Application... Filing: Notice of Intent to File License Application and Request to Use the Traditional Licensing Process. b. Project No.: P-2428-004. c. Date Filed: November 11, 2012. d. Submitted by: Aquenergy...

  3. Interoperability format translation and transformation between IFC architectural design file and simulation file formats

    Science.gov (United States)

    Chao, Tian-Jy; Kim, Younghun

    2015-01-06

    Automatically translating a building architecture file format (Industry Foundation Class) to a simulation file, in one aspect, may extract data and metadata used by a target simulation tool from a building architecture file. Interoperability data objects may be created and the extracted data is stored in the interoperability data objects. A model translation procedure may be prepared to identify a mapping from a Model View Definition to a translation and transformation function. The extracted data may be transformed using the data stored in the interoperability data objects, an input Model View Definition template, and the translation and transformation function to convert the extracted data to correct geometric values needed for a target simulation file format used by the target simulation tool. The simulation file in the target simulation file format may be generated.

  4. Interoperability format translation and transformation between IFC architectural design file and simulation file formats

    Energy Technology Data Exchange (ETDEWEB)

    Chao, Tian-Jy; Kim, Younghun

    2015-02-03

    Automatically translating a building architecture file format (Industry Foundation Class) to a simulation file, in one aspect, may extract data and metadata used by a target simulation tool from a building architecture file. Interoperability data objects may be created and the extracted data is stored in the interoperability data objects. A model translation procedure may be prepared to identify a mapping from a Model View Definition to a translation and transformation function. The extracted data may be transformed using the data stored in the interoperability data objects, an input Model View Definition template, and the translation and transformation function to convert the extracted data to correct geometric values needed for a target simulation file format used by the target simulation tool. The simulation file in the target simulation file format may be generated.

  5. File format for storage of scalable video

    Institute of Scientific and Technical Information of China (English)

    BAI Gang; SUN Xiao-yan; WU Feng; YIN Bao-cai; LI Shi-peng

    2006-01-01

    A file format for storage of scalable video is proposed in this paper. A generic model is presented to enable a codec independent description of scalable video stream. The relationships, especially the dependencies, among sub-streams in a scalable video stream are specified sufficiently and effectively in the proposed model. Complying with the presented scalable video stream model, the file format for scalable video is proposed based on ISO Base Media File Format, which is simple and flexible enough to address the demands of scalable video application as well as the non-scalable ones.

  6. [Single file endodontic treatment: a new era?].

    Science.gov (United States)

    Shay, B; Moshonov, J

    2013-04-01

    Root canal shaping is one of the key stages of endodontic treatment, when performed properly, it is a predictive factor for the outcome of the treatment. It is critical for an adequate disinfection, which is more effective at the completion of a proper shaping procedure. The introduction of NiTi instruments into endodontic greatly improved the root canal shaping and decreased the time required for a full mechanical preparation. Over the last 2 decades, numerous attempts have been made to further improve the procedure with a wide range of rotary NiTi endodontic file systems. All these systems require several subsequent files. Recently, three different concepts of single-file systems have been introduced: 1. The single file reciprocating working motion: it consists of a reciprocating counterclockwise and a clockwise motion. This reciprocal motion reduces torsional loads thus, allows safer instrumentation with less working time. 2. Single-file instrumentation using full 360 degrees rotational movement to be used in a full clockwise rotation. Some of these files also have an Anti Breakage Control (ABC) which protects from catastrophic procedural malfunction by unwinding of these file before breakage. 3. The Self-Adjusting File (SAF) which is a thin-walled, pointed cylinder, hollow nickel-titanium endodontic file that is elastically compressible into a root canal that has been previously negotiated using a #20 hand file and can widened inside it, facilitating removal of debris and dentin from the canal wall. The file conforms to the canal shape and permits irrigant flow through the file. The SAF works in a combined vibrating and partial rotational motions, and circumferentially simultaneously enlarging and irrigating the canal. The objectives of these new approaches is to reduce the working time and cost and improve safety of the shaping procedure, and suggest to have a lower procedural errors incidents. Few studies, conducted in the recent years, using these new

  7. Data Mining of NS-2 Trace File

    Directory of Open Access Journals (Sweden)

    Ahmed Jawad Kadhim

    2014-11-01

    Full Text Available Data mining is important process to extract the use ful information and pattern from huge amount of dat a. NS-2 is an efficient tool to build the environment of network. The results from simulate these environ ment in NS-2 is trace file that contains several columns and lines represent the network events. This trace file can be used to analyse the network according to per formance metrics but it has redundant columns and rows. So, this paper is to perform the data mining in order to find only the necessary information in analysis operation to reduce the execution time and the storage size of the trace file.

  8. Online file sharing innovations in media consumption

    CERN Document Server

    Andersson Schwarz, Jonas

    2013-01-01

    It is apparent that file sharing on the Internet has become an emerging norm of media consumption-especially among young people. This book provides a critical perspective on this phenomenon, exploring issues related to file sharing, downloading, peer-to-peer networks, ""piracy,"" and (not least) policy issues regarding these practices. Andersson Schwartz critically engages with the justificatory discourses of the actual file-sharers, taking Sweden as a geographic focus. By focusing on the example of Sweden-home to both The Pirate Bay and Spotify-he provides a unique insight into a mentality th

  9. Detection Of Alterations In Audio Files Using Spectrograph Analysis

    Directory of Open Access Journals (Sweden)

    Anandha Krishnan G

    2015-08-01

    Full Text Available The corresponding study was carried out to detect changes in audio file using spectrograph. An audio file format is a file format for storing digital audio data on a computer system. A sound spectrograph is a laboratory instrument that displays a graphical representation of the strengths of the various component frequencies of a sound as time passes. The objectives of the study were to find the changes in spectrograph of audio after altering them to compare altering changes with spectrograph of original files and to check for similarity and difference in mp3 and wav. Five different alterations were carried out on each audio file to analyze the differences between the original and the altered file. For altering the audio file MP3 or WAV by cutcopy the file was opened in Audacity. A different audio was then pasted to the audio file. This new file was analyzed to view the differences. By adjusting the necessary parameters the noise was reduced. The differences between the new file and the original file were analyzed. By adjusting the parameters from the dialog box the necessary changes were made. The edited audio file was opened in the software named spek where after analyzing a graph is obtained of that particular file which is saved for further analysis. The original audio graph received was combined with the edited audio file graph to see the alterations.

  10. Single file reciprocating technique using conventional nickel-titanium rotary endodontic files.

    Science.gov (United States)

    Jin, So-Youn; Lee, WooCheol; Kang, Mo K; Hur, Bock; Kim, Hyeon-Cheol

    2013-01-01

    This study was aimed to evaluate the applicability of a reciprocating movement technique with conventional nickel-titanium files for root canal preparation. Forty-four simulated canals in resin blocks were used in this study and divided as following four groups according to the instruments used and preparation methods. Group CP (n = 12) and CR (n = 12) were instrumented with continuous rotation using four files of ProFile and RaCe, respectively. Group RP (n = 10) and RR (n = 10) were instrumented with a reciprocation movement by using a single ProFile and RaCe file, respectively. The resin blocks were scanned before and after instrumentation, and the images were superimposed. To compare the efficiency of canal shaping, the preparation time, and centering ratio were calculated. Morphologic changes of tested files were examined by scanning electron microscopy (SEM). Data were analyzed by ANOVA and Duncan's post hoc test at p files used for Groups CP and CR showed no distortion under the SEM evaluation, the files used for Groups RP and RR had considerable torsional distortion. This study suggests that the reciprocating instrumentation technique using conventional nickel-titanium rotary file systems might have a comparable efficacy for the root canal shaping with reduced shaping time. Although the reciprocating technique seems to be an effective alternative to the conventional rotation technique, the risk of torsional distortion and fracture should be considered before clinical application.

  11. Museums Universe Data File (MUDF) FY 2013

    Data.gov (United States)

    Institute of Museum and Library Services — The Museum Universe Data File (MUDF) contains information about known museums in the United States using data collected and aggregated from a variety of sources.

  12. National Drug File - Reference Terminology API

    Data.gov (United States)

    U.S. Department of Health & Human Services — The National Drug File - Reference Terminology (NDF-RT) is produced by the U.S. Department of Veterans Affairs, Veterans Health Administration (VHA). NDF-RT is an...

  13. Medicare Geographic Variation - Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Geographic Variation Public Use File provides the ability to view demographic, utilization and quality indicators at the state level (including...

  14. Health Insurance Marketplace Public Use Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — A set of seven (7) public use files containing information on health insurance issuers participating in the Health Insurance Marketplace and certified qualified...

  15. 75 FR 42445 - Notice of Agreement Filed

    Science.gov (United States)

    2010-07-21

    ... No.: 012105. Title: SCM Lines Transportes/CCNI Agreement. Parties: Compania Chilena de Navegacion Interoceanica S.A. and SCM Lines Transportes Maritimos Sociedade Unipessoal, LDA. Filing Party: John P....

  16. FDIC Summary of Deposits (SOD) Download File

    Data.gov (United States)

    Federal Deposit Insurance Corporation — The FDIC's Summary of Deposits (SOD) download file contains deposit data for branches and offices of all FDIC-insured institutions. The Federal Deposit Insurance...

  17. 75 FR 2141 - Notice of Agreements Filed

    Science.gov (United States)

    2010-01-14

    ..., Inc.; Costa Cruise Lines; Crystal Cruises; Cunard Line; Disney Cruise Line; Holland America Line... Integrated Shipping Services Ltd. Filing Party: John Longstreth, Esq.; K & L Gates LLP; 1601 K Street NW...

  18. 75 FR 61757 - Notice of Agreements Filed

    Science.gov (United States)

    2010-10-06

    ... Lines, Ltd.; APL Co. PTE, Ltd.; and Mitsui O.S.K. Lines, Ltd. Filing Party: David B. Cook, Esq.; Goodwin...; Goodwin Procter LLP; 901 New York Avenue, NW., Washington, DC 20001. Synopsis: The amendment updates...

  19. NCPC Central Files Information System (CFIS)

    Data.gov (United States)

    National Capital Planning Commission — This dataset contains records from NCPC's Central Files Information System (CFIS), which is a comprehensive database of projects submitted to NCPC for design review...

  20. 78 FR 70300 - Notice of Agreements Filed

    Science.gov (United States)

    2013-11-25

    ....: 012230. Title: P3 Network Vessel Sharing Agreement. Parties: A.P. Moller-Maersk A/S trading under the name Maersk Line; CMA CGM S.A.; and MSC Mediterranean Shipping Company, S.A. Filing Party: Wayne...

  1. 75 FR 7265 - Notice of Agreement Filed

    Science.gov (United States)

    2010-02-18

    .... Moller-Maersk A/S; APL Co. Pte Ltd.; Compania Chilena de Navigacion Interoceanica, S.A.; Compania Sud... Shipping Line. Filing Party: Wayne R. Rohde, Esq.; Sher & Blackwell LLP; 1850 M Street, NW.; Suite...

  2. 76 FR 36537 - Notice of Agreement Filed

    Science.gov (United States)

    2011-06-22

    ... No.: 012130. Title: Maersk Line/HLAG Latin America Slot Exchange Agreement. Parties: A.P. Moller-Maersk A/S and Hapag-Lloyd AG. Filing Parties: Wayne Rohde, esq.; Cozen O'Connor; 1627 I Street,...

  3. FileMaker 85 Integrating the Web

    CERN Document Server

    Prosser, Susan

    2006-01-01

    FileMaker Pro, famed for power and ease of use, has added a suite of new features that can seriously boost your database productivity. This tutorial helps you take full advantage of the fresh stuff. It focuses on FileMaker's terrific new tool for integrating the Web with your databases: the Web Viewer. Step-by-step instructions help you create a Web Viewer from one of FileMaker's templates or a totally custom version of your own. But the tutorial doesn't stop there. It goes on to cover Object Naming, including FileMaker's rules for Object Names and how to use them in scripts; new scripts; ne

  4. 76 FR 12950 - Combined Notice of Filings

    Science.gov (United States)

    2011-03-09

    ... Monday, March 14, 2011. Docket Numbers: RP11-1863-000. Applicants: Stingray Pipeline Company, L.L.C. Description: Report of Stingray Pipeline Company, L.L.C. Filed Date: 03/01/2011. Accession Number:...

  5. 76 FR 51018 - Combined Notice of Filings

    Science.gov (United States)

    2011-08-17

    ... 22, 2011. Docket Numbers: RP11-2359-000. Applicants: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.204: Tres Palacios Gas Storage LLC--Revisions...

  6. 77 FR 74654 - Combined Notice of Filings

    Science.gov (United States)

    2012-12-17

    ... Existing Proceedings Docket Numbers: RP12-1068-001. Applicants: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC--Compliance with Order Accepting 587-V Filing to be effective...

  7. 78 FR 5174 - Combined Notice of Filings

    Science.gov (United States)

    2013-01-24

    ...: 20130111-5100. Comments Due: 5 p.m. ET 1/23/13. Docket Numbers: RP12-1068-002. Applicants: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC--Revised Compliance Filing to be...

  8. 77 FR 32115 - Notice of Agreements Filed

    Science.gov (United States)

    2012-05-31

    ... Agreement Western Mediterranean-U.S. East Coast. Parties: CMA CGM, S.A. and A.P. Moller-Maersk A/S. Filing.... Synopsis: The amendment would add Morocco to the geographic scope of the agreement. Agreement No.:...

  9. Source Reference File Usage: Management Summary

    Data.gov (United States)

    Social Security Administration — A report by region then by state that details how often medical sources from the Source Reference File are used when taking disability claims vs. sources that are...

  10. 77 FR 31346 - Combined Notice of Filings

    Science.gov (United States)

    2012-05-25

    ... Numbers: RP12-732-000. Applicants: Golden Triangle Storage, Inc. Description: Revisions to Request for.... Description: Negotiated Rate Service Agreement--Rice to be effective 5/16/2012. Filed Date: 5/15/12. Accession...

  11. Identifiable Data Files - Medicare Provider Analysis and ...

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Provider Analysis and Review (MEDPAR) File contains data from claims for services provided to beneficiaries admitted to Medicare certified inpatient...

  12. Identifiable Data Files - Health Outcomes Survey (HOS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Health Outcomes Survey (HOS) identifiable data files are comprised of the entire national sample for a given 2-year cohort (including both respondents...

  13. 76 FR 30336 - Combined Notice of Filings

    Science.gov (United States)

    2011-05-25

    ... Date: 5 p.m. Eastern Time on Tuesday, May 17, 2011. Docket Numbers: RP11-2094-000. Applicants: Sabine Pipe Line LLC. Description: Sabine Pipe Line LLC submits tariff filing per 154.204: Sabine Pipe...

  14. 19 CFR 10.591 - Filing procedures.

    Science.gov (United States)

    2010-04-01

    ... States Free Trade Agreement Post-Importation Duty Refund Claims § 10.591 Filing procedures. (a) Place of... preferential tariff treatment; (3) A written statement indicating whether the importer of the good provided...

  15. Energy Based Multiscale Modeling with Non-Periodic Boundary Conditions

    Science.gov (United States)

    2013-05-13

    subroutine and Python script are highlighted in Figure 14. 1. Reads the EVOL , or current element volume for all elements 2...Extracts the element stresses within the unit-cell RVE 3. Computes the volume average of the stresses based on the EVOL values 4. Using the volume

  16. Frustration and defects in non-periodic solids

    Science.gov (United States)

    Mosseri, Rémy; Sadoc, Jean-François

    2014-01-01

    Geometrical frustration arises whenever a local preferred configuration (lower energy for atomic systems, or best packing for hard spheres) cannot be propagated throughout space without defects. A general approach, using unfrustrated templates defined in curved space, have been previously applied to analyse a large number of cases like complex crystals, amorphous materials, liquid crystals, foams, and even biological organizations, with scales ranging from the atomic level up to macroscopic scales. In this paper, we discuss the close sphere packing problem, which has some relevance to the structural problem in amorphous metals, quasicrystals and some periodic complex metallic structures. The role of sets of disclination line defects is addressed, in particular with comparison with the major skeleton occurring in complex large-cell metals (Frank-Kasper phases). An interesting example of 12-fold symmetric quasiperiodic Frank-Kasper phase, and its disclination network, is also described.

  17. File level provenance tracking in CMS

    Energy Technology Data Exchange (ETDEWEB)

    Jones, C.D.; Kowalkowski, J.; Paterno, M.; Sexton-Kennedy, L.; Tanenbaum, W.; /Fermilab; Riley, D.S.; /Cornell U., LEPP

    2009-05-01

    The CMS off-line framework stores provenance information within CMS's standard ROOT event data files. The provenance information is used to track how each data product was constructed, including what other data products were read to do the construction. We will present how the framework gathers the provenance information, the efforts necessary to minimize the space used to store the provenance in the file and the tools that will be available to use the provenance.

  18. File Level Provenance Tracking in CMS

    CERN Document Server

    Jones, C D; Paterno, M; Sexton-Kennedy, L; Tanenbaum, W; Riley, D S

    2009-01-01

    The CMS off-line framework stores provenance information within CMS's standard ROOT event data files. The provenance information is used to track how each data product was constructed, including what other data products were read to do the construction. We will present how the framework gathers the provenance information, the efforts necessary to minimise the space used to store the provenance in the file and the tools that will be available to use the provenance.

  19. File level provenance tracking in CMS

    Energy Technology Data Exchange (ETDEWEB)

    Jones, C D; Kowalkowski, J; Paterno, M; Sexton-Kennedy, E; Tanenbaum, W [Fermi National Accelerator Laboratory, PO Box 500, Batavia IL 60510-5011 (United States); Riley, D S, E-mail: cdj@fnal.go [Wilson Laboratory, Cornell University, Ithaca NY 14853-8001 (United States)

    2010-04-01

    The CMS off-line framework stores provenance information within CMS's standard ROOT event data files. The provenance information is used to track how each data product was constructed, including what other data products were read to do the construction. We will present how the framework gathers the provenance information, the efforts necessary to minimise the space used to store the provenance in the file and the tools that will be available to use the provenance.

  20. Files supporting "Talking Politics on Social Media"

    OpenAIRE

    Fredheim, Rolf; Moore, Alfred

    2015-01-01

    This repository includes a description of the files needed to replicate the findings reported in 'Talking Politics on Social Media' by Rolf Fredheim and Alfred Moore --README.txt --anon.Rdata Rdata file containing deidentified commenting data needed to reproduce main figures and findings reported in the paper. --reproduction.py script used to identify reason-giving, agreement, disagreement, and insults. This script requires Python 2, version 2.5 or higher, and the modules codecs, ner, pyencha...

  1. Collective operations in a file system based execution model

    Science.gov (United States)

    Shinde, Pravin; Van Hensbergen, Eric

    2013-02-19

    A mechanism is provided for group communications using a MULTI-PIPE synthetic file system. A master application creates a multi-pipe synthetic file in the MULTI-PIPE synthetic file system, the master application indicating a multi-pipe operation to be performed. The master application then writes a header-control block of the multi-pipe synthetic file specifying at least one of a multi-pipe synthetic file system name, a message type, a message size, a specific destination, or a specification of the multi-pipe operation. Any other application participating in the group communications then opens the same multi-pipe synthetic file. A MULTI-PIPE file system module then implements the multi-pipe operation as identified by the master application. The master application and the other applications then either read or write operation messages to the multi-pipe synthetic file and the MULTI-PIPE synthetic file system module performs appropriate actions.

  2. An inconvenient truth : file-level metadata and in-file metadata caching in the (file-agnostic) ATLAS event store.

    Energy Technology Data Exchange (ETDEWEB)

    Malon, D.; van Gemmeren, P.; Hawkings, R.; Schaffer, A.; High Energy Physics; CERN; Univ. Paris-Sud

    2008-01-01

    In the ATLAS event store, files are sometimes 'an inconvenient truth.' From the point of view of the ATLAS distributed data management system, files are too small - datasets are the units of interest. From the point of view of the ATLAS event store architecture, files are simply a physical clustering optimization: the units of interest are event collections - sets of events that satisfy common conditions or selection predicates - and such collections may or may not have been accumulated into files that contain those events and no others. It is nonetheless important to maintain file-level metadata, and to cache metadata in event data files. When such metadata may or may not be present in files, or when values may have been updated after files are written and replicated, a clear and transparent model for metadata retrieval from the file itself or from remote databases is required. In this paper we describe how ATLAS reconciles its file and non-file paradigms, the machinery for associating metadata with files and event collections, and the infrastructure for metadata propagation from input to output for provenance record management and related purposes.

  3. An inconvenient truth: file-level metadata and in-file metadata caching in the (file-agnostic) ATLAS event store

    Energy Technology Data Exchange (ETDEWEB)

    Malon, D; Gemmeren, P van [Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Hawkings, R [European Organization for Nuclear Research, CERN CH-1211 Geneve 23 (Switzerland); Schaffer, A [LAL, Univ Paris-Sud, IN2P3/CNRS, Orsay (France)], E-mail: malon@anl.gov

    2008-07-15

    In the ATLAS event store, files are sometimes 'an inconvenient truth'. From the point of view of the ATLAS distributed data management system, files are too small-datasets are the units of interest. From the point of view of the ATLAS event store architecture, files are simply a physical clustering optimization: the units of interest are event collections-sets of events that satisfy common conditions or selection predicates-and such collections may or may not have been accumulated into files that contain those events and no others. It is nonetheless important to maintain file-level metadata, and to cache metadata in event data files. When such metadata may or may not be present in files, or when values may have been updated after files are written and replicated, a clear and transparent model for metadata retrieval from the file itself or from remote databases is required. In this paper we describe how ATLAS reconciles its file and non-file paradigms, the machinery for associating metadata with files and event collections, and the infrastructure for metadata propagation from input to output for provenance record management and related purposes.

  4. The Effects of Ni-Ti Hand Files, Ni-Ti Engine Files, and K-Flex Files on Root Canal Morphology

    Science.gov (United States)

    1992-09-30

    the first ni-ti root canal files that were machined from orthodontic wire. The ni-ti files were found to have two to three times more elastic... recurrent caries detection. Dentomaxillofac. Radiol.. 1992: 21:59-64. 23. Product Information and Instructions for use of Engine-Driven NT files. Dr

  5. Staphylococcus aureus detection in the mouth of housekeepers Detección de Staphylococcus aureus en la boca de trabajadores de la limpieza hospitalaria Detecção de Staphylococcus aureus na boca de trabalhadores da limpeza hospitalar

    Directory of Open Access Journals (Sweden)

    Elaine Drehmer de Almeida Cruz

    2011-02-01

    Full Text Available This study assessed the prevalence of colonization by Staphylococcus aureus in hospital housekeepers, and their knowledge and beliefs regarding this problem. Three saliva samples were collected and a questionnaire regarding knowledge and beliefs was applied. Of the 92 workers, 63 (68.5% participated in the study; 20 were not and 43 were colonized; 13 by methicillin resistant Staphylococcus aureus and 30 by methicillin sensitive Staphylococcus aureus. Persistent carrier status of methicillin resistant Staphylococcus aureus was detected in 15.4% of cases. Low knowledge and perception of occupational risk were observed. The mouth was identified as an important reservoir of methicillin resistant Staphylococcus aureus. Analyzing knowledge and beliefs, as well as the state of carrier, is an important strategy to be added to educational actions for the prevention of workers' colonization.Este estudio evaluó la prevalencia de la colonización por Staphylococcus aureus en trabajadores de limpieza hospitalaria, y su conocimiento y creencias acerca de la problemática. Fueron recolectadas tres muestras de saliva y aplicado un cuestionario referente al conocimiento y creencias. De 92 trabajadores, 63 (68,5% participaron del estudio; 20 se presentaron no colonizados y 43 colonizados; 13 para Staphylococcus aureus resistente a la meticilina y 30 para Staphylococcus aureus sensibles a la meticilina. El estado de portador persistente por Staphylococcus aureus resistente a la meticilina fue detectado en 15,4% de los casos. Bajo conocimiento y percepción del riesgo ocupacional fueron observados. La boca fue identificada como importante reservatorio de Staphylococcus aureus resistente a la meticilina. Analizar el conocimiento y creencias juntamente con la investigación del estado de portador es una importante estrategia a ser agregada a las acciones educativas para la prevención de la colonización de trabajadores.Este estudo avaliou a prevalência da coloniza

  6. 77 FR 12830 - Pershing County Water Conservation District; Notice of Intent To File License Application, Filing...

    Science.gov (United States)

    2012-03-02

    ... Pershing County, Nevada. The project occupies 0.01 acre of United States lands administered by the Bureau... Energy Regulatory Commission Pershing County Water Conservation District; Notice of Intent To File....: 14327-000. c. Date Filed: November 22, 2011. d. Submitted by: Pershing County Water...

  7. 19 CFR 149.5 - Eligibility to file an Importer Security Filing, authorized agents.

    Science.gov (United States)

    2010-04-01

    ... transmission must be an importer acting on its own behalf or a licensed customs broker. (b) Bond required. The... 19 Customs Duties 2 2010-04-01 2010-04-01 false Eligibility to file an Importer Security Filing, authorized agents. 149.5 Section 149.5 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT...

  8. 40 CFR 22.5 - Filing, service, and form of all filed documents; business confidentiality claims.

    Science.gov (United States)

    2010-07-01

    ... documents; business confidentiality claims. 22.5 Section 22.5 Protection of Environment ENVIRONMENTAL... of all filed documents; business confidentiality claims. (a) Filing of documents. (1) The original... knowledge, information and belief, the statements made therein are true, and that it is not interposed...

  9. Securing the AliEn File Catalogue - Enforcing authorization with accountable file operations

    Science.gov (United States)

    Schreiner, Steffen; Bagnasco, Stefano; Sankar Banerjee, Subho; Betev, Latchezar; Carminati, Federico; Vladimirovna Datskova, Olga; Furano, Fabrizio; Grigoras, Alina; Grigoras, Costin; Mendez Lorenzo, Patricia; Peters, Andreas Joachim; Saiz, Pablo; Zhu, Jianlin

    2011-12-01

    The AliEn Grid Services, as operated by the ALICE Collaboration in its global physics analysis grid framework, is based on a central File Catalogue together with a distributed set of storage systems and the possibility to register links to external data resources. This paper describes several identified vulnerabilities in the AliEn File Catalogue access protocol regarding fraud and unauthorized file alteration and presents a more secure and revised design: a new mechanism, called LFN Booking Table, is introduced in order to keep track of access authorization in the transient state of files entering or leaving the File Catalogue. Due to a simplification of the original Access Envelope mechanism for xrootd-protocol-based storage systems, fundamental computational improvements of the mechanism were achieved as well as an up to 50% reduction of the credential's size. By extending the access protocol with signed status messages from the underlying storage system, the File Catalogue receives trusted information about a file's size and checksum and the protocol is no longer dependent on client trust. Altogether, the revised design complies with atomic and consistent transactions and allows for accountable, authentic, and traceable file operations. This paper describes these changes as part and beyond the development of AliEn version 2.19.

  10. An in vitro comparison of torsional stress and cyclic fatigue resistance of ProFile GT and ProFile GT Series X rotary nickel-titanium files.

    Science.gov (United States)

    Kramkowski, Thomas R; Bahcall, James

    2009-03-01

    The purpose of this in vitro study was to compare the torsional stress and cyclic fatigue characteristics of ProFile GT (Dentsply Tulsa Dental, Tulsa, OK) and ProFile GT Series X (Dentsply Tulsa Dental). Files of 0.04 and 0.06 taper, 25 mm in length, and ISO sizes of 20 and 30 tips were compared (n = 25 per test group). Torque stress resistance was evaluated by measuring the torque in gram-centimeters (g-cm) and angle of deflection (degrees of rotation) required for instrument separation with use of a torsiometer instrument. Cyclic fatigue was determined by recording the time until breakage of a file rotating in a simulated canal with an applied 45 degrees or 60 degrees curve. The files were operated in a cyclic fatigue instrument that simulated clinical rotary file usage with a constant cyclical axial motion. There was no statistical difference (p > 0.05) when comparing the torque (g-cm) required to induce a torsional failure of ProFile GT and ProFile GT Series X files of identical file sizes. The angle of deflection (degrees of rotation) of ProFile GT was significantly greater (p X for all file sizes tested except 20/.04 (p > 0.05). There was no statistical difference (p > 0.05) in cyclic fatigue failure for ProFile GT and ProFile GT Series X in a canal with a curvature of 45 degrees . In the 60 degrees canal curvature, ProFile GT was found to be significantly more resistant (p = 0.005) to fracture because of cyclic fatigue than ProFile GT Series X for file size 30/.06 and significant (p files sizes 20/.06 and 30/.04. There was no difference (p > 0.05) in cyclic fatigue resistance in the 60 degrees canal for ProFile GT and ProFile GT Series X for file size 20/.04.

  11. Tracing clonality of Helicobacter pylori infecting family members from analysis of DNA sequences of three housekeeping genes (ureI, atpA and ahpC), deduced amino acid sequences, and pathogenicity-associated markers (cagA and vacA).

    Science.gov (United States)

    Owen, Robert J; Xerry, Jacqueline

    2003-06-01

    Helicobacter pylori, a Gram-negative bacterium, is a causal agent of peptic ulcers and is estimated to infect the gastric mucosa of at least half of the world's population. As primary infections are acquired mainly by household contact, studies on family clusters provide a model for investigating transmission and the natural history of initial infection. Here, sequence typing exploiting genetic variation in core fragments of three key housekeeping loci (ureI, atpA and ahpC) was used to determine clonal descent amongst isolates of ten members of four families in Northern Ireland and a family with three generations in central England. Phylogenetic analysis of each locus for 73 strains of H. pylori from 11 countries indicated high background intraspecific diversity, apart from identical paired isolates from five unrelated patients and strains with identical sequence types (STs) detected in adult members of two families. In several families carrying strains with different STs, evidence of residual clonal descent was detected at one or two loci by comparison of nucleotide and amino acid sequences. Pathogenicity-associated genotypes were heterogeneous with respect to ST and amino acid type. Analysis of these three housekeeping genes provides unique evidence for precise tracing of clonal descent in isolates of H. pylori in family groups.

  12. Image File - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ormat) for each project. One project has one pathway file originally drawn by Cell Illustrator. Data file File name: tp_atlas..._png.zip File URL: ftp://ftp.biosciencedbc.jp/archive/tp_atlas/LATEST/tp_atlas

  13. File Caving of Oracle Data File%Oracle数据文件的文件雕复

    Institute of Scientific and Technical Information of China (English)

    武振东; 汪中夏

    2013-01-01

    在系统元信息被破坏或不信任的环境下,传统数据恢复方法难以完整地恢复Oracle数据文件。为此,提出不依赖系统元信息,不使用Oracle控制文件和日志文件信息,而是基于Oracle数据文件内部结构特征进行文件雕复的数据恢复方法。采用并行唯一路径、二分碎片间隙雕复和熵值等方法进行碎片侦测,设计一种基于文件头关键字和文件地址的雕复方法。实验结果表明,该方法在无系统元信息的情况下,能有效提取Oracle数据文件,摆脱传统数据恢复对系统元信息的依赖性。%In the situation that the metadata of file system is distrusted or cannot be trusted, it is difficult to recovery the Oracle data files completely with the traditional method of data recovery. Rather than rely on the metadata of file system, Oracle control file and log file information, the file carving based on the characteristics of the Oracle data files’ internal structure is realized. File carving strategies like ideas parallel unique path, bi-fragment gap carving, entropy and so on are adopted to detect the fragment of Oracle data files. It proposes a file caving algorithm based on the keywords of the file header and file addresses. Experimental results show that this algorithm can also extract Oracle data files effectively with no metadata of file system while it gets rid of the traditional data recovery dependences on the metadata of file system.

  14. Contents of GPS Data Files

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, John P. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Carver, Matthew Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Norman, Benjamin [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-12-09

    There are no very detailed descriptions of most of these instruments in the literature – we will attempt to fix that problem in the future. The BDD instruments are described in [1]. One of the dosimeter instruments on CXD boxes is described in [2]. These documents (or web links to them) and a few others are in this directory tree. The cross calibration of the CXD electron data with RBSP is described in [3]. Each row in the data file contains the data from one time bin from a CXD or BDD instrument along with a variety of parameters derived from the data. Time steps are commandable but 4 minutes is a typical setting. These instruments are on many (but not all) GPS satellites which are currently in operation. The data come from either BDD instruments on GPS Block IIR satellites (SVN41 and 48), or else CXD-IIR instruments on GPS Block IIR and IIR-M satellites (SVN53-61) or CXD-IIF instruments on GPS block IIF satellites (SVN62-73). The CXD-IIR instruments on block IIR and IIR(M) satellites use the same design.

  15. 14 CFR 221.180 - Requirements for electronic filing of tariffs.

    Science.gov (United States)

    2010-01-01

    ... defined in this subpart. (12) The filer must provide a field in the Government Filing File for the... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Requirements for electronic filing of... Requirements for electronic filing of tariffs. (a) No carrier or filing agent shall file an electronic tariff...

  16. File access prediction using neural networks.

    Science.gov (United States)

    Patra, Prashanta Kumar; Sahu, Muktikanta; Mohapatra, Subasish; Samantray, Ronak Kumar

    2010-06-01

    One of the most vexing issues in design of a high-speed computer is the wide gap of access times between the memory and the disk. To solve this problem, static file access predictors have been used. In this paper, we propose dynamic file access predictors using neural networks to significantly improve upon the accuracy, success-per-reference, and effective-success-rate-per-reference by using neural-network-based file access predictor with proper tuning. In particular, we verified that the incorrect prediction has been reduced from 53.11% to 43.63% for the proposed neural network prediction method with a standard configuration than the recent popularity (RP) method. With manual tuning for each trace, we are able to improve upon the misprediction rate and effective-success-rate-per-reference using a standard configuration. Simulations on distributed file system (DFS) traces reveal that exact fit radial basis function (RBF) gives better prediction in high end system whereas multilayer perceptron (MLP) trained with Levenberg-Marquardt (LM) backpropagation outperforms in system having good computational capability. Probabilistic and competitive predictors are the most suitable for work stations having limited resources to deal with and the former predictor is more efficient than the latter for servers having maximum system calls. Finally, we conclude that MLP with LM backpropagation algorithm has better success rate of file prediction than those of simple perceptron, last successor, stable successor, and best k out of m predictors.

  17. 77 FR 3759 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-01-25

    ... following electric rate filings: Docket Numbers: ER12-21-002. Applicants: Agua Caliente Solar, LLC. Description: Clarification of Notice of Change in Status for Agua Caliente. Filed Date: 1/13/12....

  18. 77 FR 10737 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-02-23

    ... following electric rate filings: Docket Numbers: ER12-21-003. Applicants: Agua Caliente Solar, LLC. Description: Notice of Change in Status of Agua Caliente Solar, LLC. Filed Date: 2/14/12. Accession...

  19. 76 FR 77992 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-12-15

    ... the following foreign utility company status filings: Docket Numbers: FC12-5-000. Applicants: Bangkok Cogeneration Co., Ltd. Description: Self-Certification of FC of Bangkok Cogeneration Co., Ltd. Filed Date:...

  20. 21 CFR 1316.45 - Filings; address; hours.

    Science.gov (United States)

    2010-04-01

    ... permitted to be filed in, and correspondence relating to, hearings governed by the regulations in this chapter shall be filed with the Hearing Clerk, Drug Enforcement Administration. See the Table of DEA...

  1. 78 FR 13871 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-03-01

    ...-964-000. Applicants: EquiPower Resources Management, LLC. Description: Normal filing to be effective 4....L.C. submits tariff filing per 35.13(a)(2)(iii: Original Service Agreement No. 3488; Queue No....

  2. 76 FR 64936 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-19

    .... Applicants: Tenaska Power Management, LLC. Description: Tenaska Power Management, LLC submits tariff filing... Interconnection, L.L.C. submits tariff filing per 35.13(a)(2)(iii: PJM Queue No. M24; Original Service...

  3. 77 FR 51991 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-08-28

    ... following electric corporate filings: Docket Numbers: EC12-135-000. Applicants: Energia Sierra Juarez U.S., LLC. Description: Section 203 Application of Energia Sierra Juarez U.S., LLC. Filed Date:...

  4. 76 FR 53674 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-29

    ... following electric corporate filings: Docket Numbers: EC11-107-000. Applicants: Portsmouth Genco, LLC... Expedited Action of Portsmouth Genco, LLC. Filed Date: 08/19/2011. Accession Number: 20110819-5131....

  5. Fair Housing Act Cases Filed by Year and State

    Data.gov (United States)

    Department of Housing and Urban Development — The 'Fair Housing Act Cases Filed by Year and State with the Bases and Outcomes - 2000-2013' dataset summarizes the cases filed as a result of complaints made...

  6. Supplemental Security Income Public-Use Microdata File, 2001 Data

    Data.gov (United States)

    Social Security Administration — The SSI Public-Use Microdata File contains an extract of data fields from SSA's Supplemental Security Record file and consists of a 5 percent random, representative...

  7. 75 FR 61731 - Combined Notice of Filings #2

    Science.gov (United States)

    2010-10-06

    ... County Wind Farm LLC. Description: Benton County Wind Farm LLC submits tariff filing per 35.12: Benton.... The filings in the above proceedings are accessible in the Commission's eLibrary system by clicking on...

  8. 77 FR 45348 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-07-31

    ... following electric rate filings: Docket Numbers: ER12-2055-001. Applicants: San Gorgonio Farms, Inc... filings are accessible in the Commission's eLibrary system by clicking on the links or querying the docket...

  9. 77 FR 73027 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-12-07

    .... Description: 2501 Waverly Wind Farm, LLC GIA to be effective 11/14/ 2012. Filed Date: 11/28/12. Accession.../12. The filings are accessible in the Commission's eLibrary system by clicking on the links or...

  10. 77 FR 21760 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-04-11

    .... Docket Numbers: ER12-1433-000. Applicants: Fowler Ridge Wind Farm LLC. Description: Compliance Filing.... Comments Due: 5 p.m. ET 4/24/12. The filings are accessible in the Commission's eLibrary system by clicking...

  11. 76 FR 67434 - Combined Notice of Filings #3

    Science.gov (United States)

    2011-11-01

    .... Description: Southwest Power Pool, Inc. submits tariff filing per 35.13(a)(2)(iii): 2261 Alexander Wind Farm... Monday, November 14, 2011. The filings are accessible in the Commission's eLibrary system by clicking on...

  12. 77 FR 22566 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-04-16

    ... Farms, Inc. to be effective 3/23/2012. Filed Date: 4/6/12. Accession Number: 20120406-5097. Comments Due.... The filings are accessible in the Commission's eLibrary system by clicking on the links or querying...

  13. 29 CFR 801.71 - Filing and service.

    Science.gov (United States)

    2010-07-01

    ... EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Administrative Proceedings Modification Or Vacation of Decision and Order of Administrative Law Judge § 801.71 Filing and service. (a) Filing. All documents...

  14. 29 CFR 500.267 - Filing and service.

    Science.gov (United States)

    2010-07-01

    ... SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Modification Or Vacation of Order of Administrative Law Judge § 500.267 Filing and service. (a) Filing. All documents submitted to the Secretary...

  15. 78 FR 32383 - Combined Notice of Filings #2

    Science.gov (United States)

    2013-05-30

    ... qualifying facility filings: Docket Numbers: QF13-444-000. Applicants: The Procter & Gamble Paper Products Company. Description: Form 556 of The Procter & Gamble Paper Products Company. Filed Date: 5/21/13...

  16. FDIC Institution Directory (ID) -- Insured Insitution Download File

    Data.gov (United States)

    Federal Deposit Insurance Corporation — The FDIC's Institution Directory (ID) download file provides a list of all FDIC-insured institutions. The file includes demographic information related to the...

  17. 75 FR 23752 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-05-04

    ... received the following electric rate filings: Docket Numbers: ER06-560-008. Applicants: Credit Suisse Energy LLC. Description: Notice of Non-Material Change in Status of Credit Suisse Energy LLC. Filed Date...

  18. 76 FR 60012 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-09-28

    ... following electric rate filings: Docket Numbers: ER10-1786-003. Applicants: Credit Suisse Energy LLC. Description: Notice of Change in Status by Credit Suisse Energy LLC. Filed Date: 09/20/2011. Accession Number...

  19. 75 FR 59250 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-09-27

    ... rate filings: Docket Numbers: ER06-560-010. Applicants: Credit Suisse Energy LLC. Description: Credit Suisse Energy LLC Notice of Non-Material Change in Status. Filed Date: 09/17/2010. Accession Number...

  20. 75 FR 76721 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-12-09

    ... filing, a list of planned Project Investments and projected Project Investment costs for calendar year... per 35.13(a)(2)(iii): LGIA Mojave Solar Project SA 94 to be effective 1/30/2011. Filed Date:...

  1. 76 FR 21728 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-04-18

    .... Applicants: Stingray Pipeline Company, L.L.C. Description: Stingray Pipeline Company, L.L.C. submits tariff filing per 154.312: Stingray General Section 4 Rate Case to be effective 5/1/2011. Filed Date:...

  2. 76 FR 4099 - Combined Notice of Filings No. 2

    Science.gov (United States)

    2011-01-24

    ..., January 25, 2011. Docket Numbers: RP10-778-002. Applicants: Stingray Pipeline Company, L.L.C. Description: Stingray Pipeline Company, L.L.C. submits tariff filing per 154.203: Baseline Compliance Filing to...

  3. 75 FR 54864 - Combined Notice of Filings No. 2

    Science.gov (United States)

    2010-09-09

    ...: Tres Palacios Gas Storage LLC. Description: Tres Palacios Gas Storage LLC submits tariff filing per 154.203: Tres Palacios Gas Storage LLC--Compliance Filing Order No. 587-U to be effective 11/1/2010....

  4. 76 FR 22686 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-04-22

    ... proceedings must file in accordance with Rules 211 and 214 of the Commission's Rules of Practice and Procedure...: Market based rate tariff database to be effective 4/ 15/2011. Filed Date: 04/14/2011. Accession...

  5. 75 FR 74707 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-12-01

    ... proceedings must file in accordance with Rules 211 and 214 of the Commission's Rules of Practice and Procedure...: Cancel Tariff Database to be effective 1/10/2011. Filed Date: 11/12/2010. Accession Number:...

  6. 29 CFR 1980.103 - Filing of discrimination complaint.

    Science.gov (United States)

    2010-07-01

    ... THE CORPORATE AND CRIMINAL FRAUD ACCOUNTABILITY ACT OF 2002, TITLE VIII OF THE SARBANES-OXLEY ACT OF... communication will be considered to be the date of filing; if the complaint is filed in person, by...

  7. 75 FR 38804 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-07-06

    ... wholesale generator filings: Docket Numbers: EG10-49-000. Applicants: Synergics Roth Rock Wind Energy, LLC. Description: Self-Certification of EWG Status of Synergics Roth Rock Wind Energy, LLC. Filed Date:...

  8. 14 CFR 248.4 - Time for filing reports.

    Science.gov (United States)

    2010-01-01

    ...) ECONOMIC REGULATIONS SUBMISSION OF AUDIT REPORTS § 248.4 Time for filing reports. The report required by this part shall be filed with the Office of Airline Information within 15 days after the due date of...

  9. 75 FR 62522 - Combined Notice of Filings No. 3

    Science.gov (United States)

    2010-10-12

    .... Description: CenterPoint Energy Gas Transmission Company submits tariff filing per 154.203: NAESB EDI Form... River Transmission Corporation submits tariff filing per 154.203: NAESB 1.9 EDI Form to be effective...

  10. Medical Expenditure Panel Survey (MEPS) Restricted Data Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — Restricted Data Files Available at the Data Centers Researchers and users with approved research projects can access restricted data files that have not been...

  11. 76 FR 30332 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-05-25

    ... Report filings: Docket Numbers: RP11-2094-000. Applicants: Sabine Pipe Line LLC. Description: Sabine Pipe Line LLC submits tariff filing per 154.204: Sabine Pipe Line LLC System Map to be effective...

  12. 76 FR 69252 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-11-08

    ... following exempt wholesale generator filings: Docket Numbers: EG12-7-000. Applicants: Manzana Wind LLC. Description: Self-Certification of Exempt Wholesale Generator Status of Manzana Wind LLC. Filed Date:...

  13. 76 FR 61351 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-04

    ... Monday, October 3, 2011. Docket Numbers: ER11-4606-000. Applicants: Dragon Energy, LLC. Description: Dragon Energy, LLC submits tariff filing per 35.1: FERC Electric MBR Baseline Tariff Filing to...

  14. WFC3/UVIS: Updates to SYNPHOT Reference Files and IMPHTTAB

    Science.gov (United States)

    Deustua, S. E.; Bajaj, V.

    2017-05-01

    Updates since February 2016 to SYNPHOT configuration reference files and the photometry lookup up table, IMPHTTAB, for WFC3/UVIS are described. We also document the history and description of changes implemented for previous versions of the same reference files.

  15. 75 FR 70227 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-17

    ... Operations Company submits tariff filing per 35: GMO Order 739 Compliance Filing (Section 23.1) to be... FERC Online service, please e-mail FERCOnlineSupport@ferc.gov or call (866) 208-3676 (toll free). For...

  16. Bin-Carver: Automatic Recovery of Binary Executable Files

    Science.gov (United States)

    2012-05-01

    wild daily, recovery of binary executable files becomes an important problem, especially for the case in which malware deletes itself after compromising...traditional file carving mainly focuses on document and image files such as PDF and JPEG. With the vast amount of malware code appearing in the wild ...Annual Network and Distributed System Security Symposium (NDSS’12), San Diego, CA, 2012. [18] M. Karresand, N. Shahmehri, Oscar – file type identification

  17. ESUSA: U. S. endangered species distribution file

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, J; Calef, C E

    1978-05-01

    A file containing distribution data on federally listed or proposed endangered species of the United States is described. Included are (a) the common name, (b) the scientific name, (c) the taxonomic family, (d) the OES/FWS/USDI group (mammal, bird, etc.), (e) the status, (f) the geographic distribution by counties, and (g) Federal Register references. Status types are endangered, threatened, proposed, under review, deleted, and rejected. Distribution is by Federal Information Processing Standard (FIPS) county code and is of four types: designated critical habitat, present range, potential range, and historic range. The file is currently being used in conjunction with similar data on projected future energy facilities to anticipate possible conflicts. However, the file would be useful to any project correlating endangered species with location information expressed by county. An example is as an aid in evaluating Forest Service or Bureau of Land Management proposed wilderness areas.

  18. Auto Draw from Excel Input Files

    Science.gov (United States)

    Strauss, Karl F.; Goullioud, Renaud; Cox, Brian; Grimes, James M.

    2011-01-01

    The design process often involves the use of Excel files during project development. To facilitate communications of the information in the Excel files, drawings are often generated. During the design process, the Excel files are updated often to reflect new input. The problem is that the drawings often lag the updates, often leading to confusion of the current state of the design. The use of this program allows visualization of complex data in a format that is more easily understandable than pages of numbers. Because the graphical output can be updated automatically, the manual labor of diagram drawing can be eliminated. The more frequent update of system diagrams can reduce confusion and reduce errors and is likely to uncover symmetric problems earlier in the design cycle, thus reducing rework and redesign.

  19. Tuning HDF5 for Lustre File Systems

    Energy Technology Data Exchange (ETDEWEB)

    Howison, Mark; Koziol, Quincey; Knaak, David; Mainzer, John; Shalf, John

    2010-09-24

    HDF5 is a cross-platform parallel I/O library that is used by a wide variety of HPC applications for the flexibility of its hierarchical object-database representation of scientific data. We describe our recent work to optimize the performance of the HDF5 and MPI-IO libraries for the Lustre parallel file system. We selected three different HPC applications to represent the diverse range of I/O requirements, and measured their performance on three different systems to demonstrate the robustness of our optimizations across different file system configurations and to validate our optimization strategy. We demonstrate that the combined optimizations improve HDF5 parallel I/O performance by up to 33 times in some cases running close to the achievable peak performance of the underlying file system and demonstrate scalable performance up to 40,960-way concurrency.

  20. Next generation WLCG File Transfer Service (FTS)

    CERN Document Server

    CERN. Geneva

    2012-01-01

    LHC experiments at CERN and worldwide utilize WLCG resources and middleware components to perform distributed computing tasks. One of the most important tasks is reliable file replication. It is a complex problem, suffering from transfer failures, disconnections, transfer duplication, server and network overload, differences in storage systems, etc. To address these problems, EMI and gLite have provided the independent File Transfer Service (FTS) and Grid File Access Library (GFAL) tools. Their development started almost a decade ago, in the meantime, requirements in data management have changed - the old architecture of FTS and GFAL cannot keep support easily these changes. Technology has also been progressing: FTS and GFAL do not fit into the new paradigms (cloud, messaging, for example). To be able to serve the next stage of LHC data collecting (from 2013), we need a new generation of  these tools: FTS 3 and GFAL 2. We envision a service requiring minimal configuration, which can dynamically adapt to the...

  1. 20 CFR 655.15 - Required pre-filing recruitment.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Required pre-filing recruitment. 655.15... States (H-2B Workers) § 655.15 Required pre-filing recruitment. (a) Time of filing of application. An... recruitment steps set forth in this section have been fully satisfied, except where specifically exempted from...

  2. 77 FR 53883 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-09-04

    ... filings: Docket Numbers: ER12-1470-001. Applicants: Energia Sierra Juarez U.S., LLC. Description: Energia Sierra Juarez U.S. LLC Revised MBR Tariff to be effective 10/1/2012. Filed Date: 8/13/12. Accession... Service Company of New Mexico. Description: OATT Attachment R Compliance Filing to be effective 7/...

  3. 77 FR 1483 - Notice of Filing; City of Anaheim, CA

    Science.gov (United States)

    2012-01-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Filing; City of Anaheim, CA Take notice that on December 14, 2011, City of Anaheim, California submitted its tariff filing per 35.28(e): 2012 TRBAA Update Filing, to...

  4. 77 FR 1483 - Notice of Filing; City of Azusa, CA

    Science.gov (United States)

    2012-01-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Filing; City of Azusa, CA Take notice that on December 29, 2011, City of Azusa, California submitted its tariff filing per 35.28(e): 2012 TRBAA Update Filing, to...

  5. 77 FR 1484 - Notice of Filing; City of Pasadena, CA

    Science.gov (United States)

    2012-01-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Filing; City of Pasadena, CA Take notice that on December 14, 2011, City of Pasadena, California submitted its tariff filing per 35.28(e): 2012 TRBAA Update Filing, to...

  6. 19 CFR 177.26 - Where request filed.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Where request filed. 177.26 Section 177.26 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... request filed. The request shall be filed with the Executive Director, Regulations and Rulings, Office...

  7. Digital Libraries: The Next Generation in File System Technology.

    Science.gov (United States)

    Bowman, Mic; Camargo, Bill

    1998-01-01

    Examines file sharing within corporations that use wide-area, distributed file systems. Applications and user interactions strongly suggest that the addition of services typically associated with digital libraries (content-based file location, strongly typed objects, representation of complex relationships between documents, and extrinsic…

  8. 75 FR 79364 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-12-20

    ...): Piedmont Green Power LGIA Amendment Filing to be effective 9/23/2010. Filed Date: 12/02/2010. Accession.... Applicants: Brookfield Energy Marketing Inc. Description: Brookfield Energy Marketing Inc. submits tariff filing per 35.13(a)(2)(iii): Brookfield Energy Marketing Inc. Market-Based Rate Tariff to be effective 12...

  9. 19 CFR 191.0a - Claims filed under NAFTA.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Claims filed under NAFTA. 191.0a Section 191.0a Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) DRAWBACK § 191.0a Claims filed under NAFTA. Claims for drawback filed under the...

  10. 77 FR 16827 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-03-22

    ... following electric corporate filings: Docket Numbers: EC12-80-000. Applicants: Pioneer Trail Wind Farm, LLC... Filing Requirements, Expedited Review And Confidential Treatment of Pioneer Trail Wind Farm, LLC. Filed... the Commission's eLibrary system by clicking on the links or querying the docket number. Any person...

  11. 76 FR 32182 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-06-03

    ... following exempt wholesale generator filings: Docket Numbers: EG11-87-000. Applicants: Sherbino II Wind Farm... Wind Farm LLC. Filed Date: 05/26/2011. Accession Number: 20110526-5128. ] Comment Date: 5 p.m. Eastern.... The filings in the above proceedings are accessible in the Commission's eLibrary system by clicking on...

  12. 76 FR 51019 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-17

    ... following exempt wholesale generator filings: Docket Numbers: ER11-2935-002. Applicants: Paulding Wind Farm II LLC. Description: Notice of Non-Material Change in Status of Paulding Wind Farm II LLC. Filed Date... Date: None Applicable. The filings are accessible in the Commission's eLibrary system by clicking on...

  13. 76 FR 12353 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-03-07

    ... Washington, NC Walmart. Filed Date: 07/02/2010. Accession Number: 20100702-5029. Comment Date: None... PowerSecure Inc. at Laurinburg, NC Walmart. Filed Date: 07/02/2010. Accession Number: 20100702-5032...: Self-Certification of PowerSecure Inc. at Wilson, NC Walmart. Filed Date: 07/02/2010. Accession...

  14. 47 CFR 1.1705 - Forms; electronic and manual filing.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Forms; electronic and manual filing. 1.1705... Operations and Licensing System (COALS) § 1.1705 Forms; electronic and manual filing. (a) Application forms... via COALS in Adobe Acrobat Portable Document Format (PDF) whenever possible. (c) Manual filing....

  15. 30 CFR 706.15 - Where to file.

    Science.gov (United States)

    2010-07-01

    ... file his or her statement of employment and financial interests with the Director. (b) Each Department... the Act, shall file a statement of employment and financial interests with his or her appropriate... performs a function or duty under the Act shall file a statement of employment and financial interests...

  16. 20 CFR 404.632 - Statements filed with a hospital.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Statements filed with a hospital. 404.632... § 404.632 Statements filed with a hospital. A statement (generally a hospital admission form) filed with a hospital may serve as a written statement under § 404.630 if the requirements of this section are...

  17. 76 FR 69263 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-11-08

    ... submits tariff filing per 35.13(a)(2)(i): MUN-1 2012 Small Rate Increase to be effective 1/1/ 2012. Filed... Marketing Affiliates. Description: Request Of Virginia Electric And Power Company And Its Market-Regulated Power Sales Affiliates For Waivers Of Certain Affiliate Restrictions Requirement. Filed Date: 11/01/2011...

  18. 30 CFR 28.30 - Quality control plans; filing requirements.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Quality control plans; filing requirements. 28... PROTECTION FOR TRAILING CABLES IN COAL MINES Quality Control § 28.30 Quality control plans; filing... part, each applicant shall file with MSHA a proposed quality control plan which shall be designed...

  19. 28 CFR 10.5 - Incorporation of papers previously filed.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Incorporation of papers previously filed... CARRYING ON ACTIVITIES WITHIN THE UNITED STATES Registration Statement § 10.5 Incorporation of papers previously filed. Papers and documents already filed with the Attorney General pursuant to the said act...

  20. 17 CFR 201.152 - Filing of papers: Form.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Filing of papers: Form. 201... PRACTICE Rules of Practice General Rules § 201.152 Filing of papers: Form. (a) Specifications. Papers filed... white paper measuring 81/2×11 inches, except that, to the extent that the reduction of larger...

  1. 45 CFR 99.5 - Filing and service of papers.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Filing and service of papers. 99.5 Section 99.5... THE CHILD CARE AND DEVELOPMENT FUND General § 99.5 Filing and service of papers. (a) An original and two copies of all papers in the proceedings shall be filed with the presiding officer. For...

  2. 10 CFR 13.26 - Filing and service of papers.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Filing and service of papers. 13.26 Section 13.26 Energy NUCLEAR REGULATORY COMMISSION PROGRAM FRAUD CIVIL REMEDIES § 13.26 Filing and service of papers. (a... paragraph (a)(1) of this section. Such a participant will file paper documents physically or by mail to...

  3. 37 CFR 11.41 - Filing of papers.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Filing of papers. 11.41... Disciplinary Proceedings; Jurisdiction, Sanctions, Investigations, and Proceedings § 11.41 Filing of papers. (a... papers filed after the complaint and prior to entry of an initial decision by the hearing officer...

  4. 12 CFR 308.158 - Filing papers and effective date.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Filing papers and effective date. 308.158 Section 308.158 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE... Section 19 of the FDIA § 308.158 Filing papers and effective date. (a) Filing with the regional...

  5. 32 CFR 150.5 - Place for filing papers.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false Place for filing papers. 150.5 Section 150.5... MILITARY JUSTICE COURTS OF CRIMINAL APPEALS RULES OF PRACTICE AND PROCEDURE § 150.5 Place for filing papers. When the filing of a notice of appearance, brief, or other paper in the office of a Judge...

  6. 49 CFR 1152.24 - Filing and service of application.

    Science.gov (United States)

    2010-10-01

    ... be submitted to cover the applicable filing fee. If the applicant carrier is in bankruptcy, the application shall also be filed on the bankruptcy court. (b) The applicant shall tender with its application... which business for the line is received or forwarded). A certificate of service shall be promptly filed...

  7. 75 FR 6377 - Dynegy Services Plum Point; Notice of Filing

    Science.gov (United States)

    2010-02-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Dynegy Services Plum Point; Notice of Filing February 2, 2010. Take notice that, on February 1, 2010, Dynegy Services Plum Point filed to supplement its filing in the...

  8. 77 FR 62505 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-10-15

    ... the following exempt wholesale generator filings: Docket Numbers: EG13-1-000. Applicants: Big Blue Wind Farm, LLC. Description: Self-Certification of EWG Status of Big Blue Wind Farm, LLC. Filed Date... and Related Requests of LDH Rensselaer LLC and Louis Dreyfus Energy Services, L.P. Filed Date:...

  9. 75 FR 56085 - Combined Notice of Filings # 1

    Science.gov (United States)

    2010-09-15

    ... Numbers: ER10-1521-001 Applicants: Occidental Power Marketing, L.P. Description: Occidental Power Marketing, L.P. submits tariff filing per 35: Change in Status to be effective 9/8/2010. Filed Date: 09/07...: Black Hills Power, Inc. Description: Black Hills Power, Inc. submits tariff filing per 35.12:...

  10. 78 FR 76605 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-18

    ...-2013 SA 2017 G540/G548 Barton-ITC GIA Compliance Filing to be effective 11/6/2013. Filed Date: 12/10/13.... submits 2635 Lincoln Electric System GIA to be effective 11/26/2013. Filed Date: 12/10/13....

  11. 76 FR 31600 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-06-01

    ..., Inc. submits tariff filing per 35.17(b): J143 GIA Amendment, to be effective 3/19/2011. File Date: 05... Power Pool, Inc. submits tariff filing per 35.13(a)(2)(iii: 2208 Ensign Wind, LLC GIA to be effective...

  12. 76 FR 21723 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-04-18

    ... Independent Transmission System Operator, Inc. submits tariff filing per 35.13(a)(2)(iii: G931 Amended GIA to... tariff filing per 35.13(a)(2)(iii: G996 Amended GIA to be effective 4/9/2011. Filed Date:...

  13. 76 FR 42704 - Sky River LLC; Notice of Filing

    Science.gov (United States)

    2011-07-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Sky River LLC; Notice of Filing Take notice that, on July 8, 2011, Sky River LLC filed to amend its Open Access Transmission Tariff (OATT) filing, submitted on April 1, 2011...

  14. 48 CFR 2904.800-70 - Contents of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Contents of contract files. 2904.800-70 Section 2904.800-70 Federal Acquisition Regulations System DEPARTMENT OF LABOR GENERAL ADMINISTRATIVE MATTERS Government Contract Files 2904.800-70 Contents of contract files. (a) The reports...

  15. 48 CFR 1304.804 - Closeout of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Closeout of contract files. 1304.804 Section 1304.804 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL ADMINISTRATIVE MATTERS Government Contract Files 1304.804 Closeout of contract files....

  16. 48 CFR 4.804 - Closeout of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Closeout of contract files. 4.804 Section 4.804 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL ADMINISTRATIVE MATTERS Government Contract Files 4.804 Closeout of contract files....

  17. 48 CFR 904.803 - Contents of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contents of contract files. 904.803 Section 904.803 Federal Acquisition Regulations System DEPARTMENT OF ENERGY GENERAL ADMINISTRATIVE MATTERS Government Contract Files 904.803 Contents of contract files. (a) (29) The record copy...

  18. 48 CFR 204.805 - Disposal of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Disposal of contract files. 204.805 Section 204.805 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE GENERAL ADMINISTRATIVE MATTERS Contract Files 204.805 Disposal of contract files....

  19. 48 CFR 1504.804 - Closeout of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Closeout of contract files. 1504.804 Section 1504.804 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY GENERAL ADMINISTRATIVE MATTERS Contract Files 1504.804 Closeout of contract files....

  20. 48 CFR 904.804 - Closeout of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Closeout of contract files. 904.804 Section 904.804 Federal Acquisition Regulations System DEPARTMENT OF ENERGY GENERAL ADMINISTRATIVE MATTERS Government Contract Files 904.804 Closeout of contract files....

  1. 48 CFR 1404.804 - Closeout of contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Closeout of contract files. 1404.804 Section 1404.804 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.804 Closeout of contract files....

  2. 75 FR 32453 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-06-08

    ... received the following electric rate filings: Docket Numbers: ER10-1093-001. Applicants: Delaware City Refining Company LLC. Description: Delaware City Refining Company LLC submits tariff filing per 35.17(b...: PBF Power Marketing LLC. Description: PBF Power Marketing LLC submits tariff filing per...

  3. 77 FR 30272 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-05-22

    .... 3319; Queue No. W4- 004A--AT10 to be effective 5/1/2012. Filed Date: 5/14/12. Accession Number... System Operator Corporation. Description: 2012-05-14 CAISO Credit Reforms Comp Filing to be effective 4... filings are accessible in the Commission's eLibrary system by clicking on the links or querying the...

  4. 77 FR 3468 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-01-24

    ...-701-001. Applicants: New York Independent System Operator, Inc. Description: New York Independent System Operator, Inc. submits tariff filing per 35.17(b): NYISO Amendment to 12/28/11 Filing re... Service Agreement No. 3171; Queue Position W1-127 to be effective 12/13/2011. Filed Date:...

  5. 78 FR 67355 - Combined Notice of Filings #2

    Science.gov (United States)

    2013-11-12

    ... Distribution Service Agmt with NRG Solar Blythe II LLC to be effective 12/31/2013. Filed Date: 10/31/13...--Wine Country to be effective 12/31/2013. Filed Date: 10/31/13. Accession Number: 20131031-5169..., protests, service, and qualifying facilities filings can be found at:...

  6. 76 FR 17642 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-30

    ... Central Company. Description: AEP Texas Central Company submits tariff filing per 35.15: 20110322 TCC...): 20110322 TCC-Magic Valley GIA to be effective 2/25/ 2011. Filed Date: 03/22/2011. Accession Number...(a)(2)(iii): 20110322 TCC-Los Vientos GIA to be effective 3/7/ 2011. Filed Date:...

  7. 76 FR 76961 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-12-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice that the Commission received the... Participants Committee. Description: Dec 2011 Membership Filing to be effective 11/1/2011. Filed Date:...

  8. 77 FR 53801 - Nexira; Filing of Food Additive Petition; Amendment

    Science.gov (United States)

    2012-09-04

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 172 Nexira; Filing of Food Additive Petition... Administration (FDA) is amending the filing notice for a food additive petition filed by Nexira proposing that the food additive regulations be amended to provide for the expanded safe use of acacia gum...

  9. 77 FR 5201 - Ecolab, Inc.; Filing of Food Additive Petition

    Science.gov (United States)

    2012-02-02

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 173 Ecolab, Inc.; Filing of Food Additive... Administration (FDA) is announcing that Ecolab, Inc., has filed a petition proposing that the food additive...) (21 U.S.C. 348(b)(5))), notice is given that a food additive petition (FAP 2A4785) has been filed...

  10. 76 FR 78866 - Nexira; Filing of Food Additive Petition

    Science.gov (United States)

    2011-12-20

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 172 Nexira; Filing of Food Additive Petition... Administration (FDA) is announcing that Nexira has filed a petition proposing that the food additive regulations... given that a food additive petition (FAP 1A4784) has been filed by Nexira, c/o Keller and Heckman...

  11. 46 CFR 232.6 - Financial report filing requirement.

    Science.gov (United States)

    2010-10-01

    ... RELATED ACTIVITIES UNIFORM FINANCIAL REPORTING REQUIREMENTS Income Statement § 232.6 Financial report filing requirement. (a) Reporting Frequency and Due Dates. The contractor shall file a semiannual... 46 Shipping 8 2010-10-01 2010-10-01 false Financial report filing requirement. 232.6 Section...

  12. 29 CFR 409.3 - Time for filing annual report.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 2 2010-07-01 2010-07-01 false Time for filing annual report. 409.3 Section 409.3 Labor... STANDARDS REPORTS BY SURETY COMPANIES § 409.3 Time for filing annual report. Each surety company required to file an annual report by section 211 of the Labor-Management Reporting and Disclosure Act of 1959...

  13. 29 CFR 4003.53 - Where to file.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Where to file. 4003.53 Section 4003.53 Labor Regulations... OF AGENCY DECISIONS Administrative Appeals § 4003.53 Where to file. An appeal or a request for an... additional information on where to file....

  14. 43 CFR 4.1301 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1301 Section 4.1301 Public... Individual Civil Penalty Assessments Under Section 518(f) of the Act § 4.1301 Who may file. Any individual served a notice of proposed individual civil penalty assessment may file a petition for review with...

  15. 43 CFR 4.1161 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1161 Section 4.1161 Public... Violation and Orders of Cessation § 4.1161 Who may file. A permittee issued a notice or order by the....1160 may file an application for review with the Hearings Division, OHA, 801 North Quincy...

  16. 43 CFR 4.1361 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1361 Section 4.1361 Public... Lands) and for Coal Exploration Permits (federal Program) § 4.1361 Who may file. The applicant... forth in § 4.1360 may file a request for review of that decision....

  17. 27 CFR 24.117 - Maintenance of application file.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Maintenance of application file. 24.117 Section 24.117 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Maintenance of application file. The proprietor shall maintain an application file with the...

  18. 43 CFR 4.1290 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1290 Section 4.1290 Public Lands: Interior Office of the Secretary of the Interior DEPARTMENT HEARINGS AND APPEALS PROCEDURES... Expenses Under Section 525(e) of the Act § 4.1290 Who may file. (a) Any person may file a petition...

  19. 43 CFR 4.1150 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1150 Section 4.1150 Public Lands: Interior Office of the Secretary of the Interior DEPARTMENT HEARINGS AND APPEALS PROCEDURES... Assessments of Civil Penalties § 4.1150 Who may file. Any person charged with a civil penalty may file...

  20. 10 CFR 205.13 - Where to file.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Where to file. 205.13 Section 205.13 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS General Provisions § 205.13 Where to file. (a) Except as otherwise specifically provided in other subparts of this part, all documents to be filed with...