WorldWideScience

Sample records for non-ortho cbs measured

  1. CBS Kilen

    DEFF Research Database (Denmark)

    2006-01-01

    Præsentation af CBS Kilen ved landskabsarkitekt Marianne Levinsen med arkitekturfotografier af bl.a. Christina Capatillo......Præsentation af CBS Kilen ved landskabsarkitekt Marianne Levinsen med arkitekturfotografier af bl.a. Christina Capatillo...

  2. CBS-uddannelser

    DEFF Research Database (Denmark)

    van der Meer, Wilbert; Mondahl, Margrethe

    2016-01-01

    Berlingske bragte 20.04 artiklen »Nyuddannede CBS'ere kæmper med høj arbejdsløshed«. Artiklen kan efterlade det indtryk, at CBS på trods af relativt svage beskæftigelsestal for erhvervssproglige dimittender ikke tilpasser optag til efterspørgsel.......Berlingske bragte 20.04 artiklen »Nyuddannede CBS'ere kæmper med høj arbejdsløshed«. Artiklen kan efterlade det indtryk, at CBS på trods af relativt svage beskæftigelsestal for erhvervssproglige dimittender ikke tilpasser optag til efterspørgsel....

  3. En vision for CBS?

    DEFF Research Database (Denmark)

    Thyssen, Ole

    2015-01-01

    Kommentar. CBS’ ry for at være et moderne Business University med forskere fra hele verden og forskningsmæssig dynamik faldt på gulvet. Udfordringen er nu at få samlet CBS forskere om en fælles vision.......Kommentar. CBS’ ry for at være et moderne Business University med forskere fra hele verden og forskningsmæssig dynamik faldt på gulvet. Udfordringen er nu at få samlet CBS forskere om en fælles vision....

  4. Declining trends of polychlorinated dibenzo-p-dioxins, dibenzofurans and non-ortho PCBs in Canadian Arctic seabirds.

    Science.gov (United States)

    Braune, Birgit M; Mallory, Mark L

    2017-01-01

    Polychorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (PCBs) such as the non-ortho PCBs (nPCBs) persist in the environment despite international measures to ban their emissions. We determined congener patterns and temporal trends for PCDDs, PCDFs, nPCBs as well as their toxic equivalents (TEQs) in eggs of thick-billed murres (Uria lomvia) and northern fulmars (Fulmarus glacialis) sampled from Prince Leopold Island in the Canadian Arctic between 1975 and 2014. The dominant PCDD congeners were 1,2,3,7,8-PnCDD, 2,3,7,8-TCDD and 1,2,3,6,7,8-HxCDD, and the dominant PCDF congener was 2,3,4,7,8-PnCDF. The nPCB profile was dominated by PCB-126. The TEQ profile in the murre eggs was dominated by nPCB-TEQ whereas in the fulmar eggs, the PCDF-TEQ contribution to ΣTEQ was slightly greater than that of nPCB-TEQ. Concentrations of ΣPCDD, ΣPCDF, ΣnPCB and ΣTEQ declined between 1975 and 2014 in both murre and fulmar eggs. Based on TEQ thresholds in the literature for other species, and taking into account the trend towards declining TEQ levels, it is unlikely that current levels of PCDDs, PCDFs or nPCBs are affecting the reproductive success of thick-billed murres or northern fulmars in the Canadian Arctic.

  5. Transparens på CBS?

    DEFF Research Database (Denmark)

    Mouritsen, Johannes

    2015-01-01

    Debatindlæg. Hvad er grundlaget for CBS' strategi? Indlægget handler om transparens i organisationer. Det begynder med to cases fra biosektoren og ender med kommentarer til mangel på transparens på CBS.......Debatindlæg. Hvad er grundlaget for CBS' strategi? Indlægget handler om transparens i organisationer. Det begynder med to cases fra biosektoren og ender med kommentarer til mangel på transparens på CBS....

  6. Transparens på CBS?

    DEFF Research Database (Denmark)

    Mouritsen, Johannes

    2015-01-01

    Debatindlæg. Hvad er grundlaget for CBS' strategi? Indlægget handler om transparens i organisationer. Det begynder med to cases fra biosektoren og ender med kommentarer til mangel på transparens på CBS.......Debatindlæg. Hvad er grundlaget for CBS' strategi? Indlægget handler om transparens i organisationer. Det begynder med to cases fra biosektoren og ender med kommentarer til mangel på transparens på CBS....

  7. Levels and congener distributions of PCDDs, PCDFs and non-ortho PCBs in Belgian foodstuffs--assessment of dietary intake.

    Science.gov (United States)

    Focant, J F; Eppe, G; Pirard, C; Massart, A C; André, J E; De Pauw, E

    2002-07-01

    Congener-specific analyses of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 4 non-ortho (coplanar) polychlorinated biphenyls (cPCBs) were performed on 197 foodstuffs samples of animal origin from Belgium during years 2000 and 2001. All investigated matrices (except horse) present background levels lower than the Belgian non-commercialization value of 5 pg TEQ/g fat. Pork was the meat containing the lowest concentration of both PCDD/Fs and cPCBs. The mean background concentration of 2,3,7,8-TCDD toxicity equivalent in milk was 1.1 pg/g of fat, with a congener distribution typical of non-contaminated milk. The relative contribution of 2,3,7,8-TCDD, 2,3,7,8-TCDF, 1,2,3,7,8-PeCDD and 2,3,4,7,8-PeCDF to the PCDD/Fs TEQ was 85+/-7.9% for all investigated matrices. The cPCBs contribution to the total TEQ was 47+/-19.0% for products of terrestrial species and 69+/-20.0% for aquatic species. Once the contribution of cPCBs was added to the TEQ, few foodstuffs such as horse, sheep, beef, eggs and cheese presented levels above the future European guidelines that currently only include PCDD/Fs but will be re-evaluated later in order to include 'dioxin-like' PCBs. Based on levels measured in the samples, the estimation of the dietary intake was 65.3 pg WHO-TEQ/day for PCDD/Fs only (1.00 pg WHO-TEQ/kg bw/day, for a 65 kg person) and 132.9 pg WHO-TEQ/day if cPCBs were included (2.04 pg WHO-TEQ/kg bw/day, for a 65 kg person). Meat (mainly beef), dairy products, and fish each account for roughly one third of the intake.

  8. NOCBS -- Use of CBS mail on satellites

    Science.gov (United States)

    Allan, P. M.

    Since the introduction of version 5 of VMS and the Coloured Books software (CBS), it has been possible to send CBS mail from within VMS mail by using an address of the form "CBS%nodename::username". This only works on cluster nodes that have CBS installed. The usual situation at Starlink sites that have VAXclusters is that CBS is installed on only one machine in the cluster.

  9. Lack of global epigenetic methylation defects in CBS deficient mice.

    Science.gov (United States)

    Lee, Hyung-Ok; Wang, Liqun; Kuo, Yin-Ming; Gupta, Sapna; Slifker, Michael J; Li, Yue-Sheng; Andrews, Andrew J; Kruger, Warren D

    2017-01-01

    Cystathionine β-synthase (CBS) deficiency is a recessive inborn error of metabolism in which patients have extremely elevated plasma total homocysteine and have clinical manifestations in the vascular, visual, skeletal, and nervous systems. Homocysteine is an intermediary metabolite produced from the hydrolysis of S-adenosylhomocysteine (SAH), which is a by-product of methylation reactions involving the methyl-donor S-adenosylmethionine (SAM). Here, we have measured SAM, SAH, DNA and histone methylation status in an inducible mouse model of CBS deficiency to test the hypothesis that homocysteine-related phenotypes are caused by inhibition of methylation due to elevated SAH and reduced SAM/SAH ratio. We found that mice lacking CBS have elevated cellular SAH and reduced SAM/SAH ratios in both liver and kidney, but this was not associated with alterations in the level of 5-methylcytosine or various histone modifications. Using methylated DNA immunoprecipitation in combination with microarray, we found that of the 241 most differentially methylated promoter probes, 89 % were actually hypermethylated in CBS deficient mice. In addition, we did not find that changes in DNA methylation correlated well with changes in RNA expression in the livers of induced and uninduced CBS mice. Our data indicates that reduction in the SAM/SAH ratio, due to loss of CBS activity, does not result in overall hypomethylation of either DNA or histones.

  10. On semantics-based spatial data preprocessing: a case study in non-ortho RS images mosaic

    Science.gov (United States)

    Wang, Daojun; Gong, Jianhua; Ma, Ai-Nai

    2008-10-01

    A Three-Level Information Architecture containing Syntactic Information, Semantic Information and Pragmatic Information is put forward in Comprehensive Information Theory (CIT). From this point of view, spatial data analysis is in cooperation with semantic information and Spatial Data Preprocessing (SDP) is corresponding to syntactic information. However, in many practical applications, SDP based only on syntactic information can not get a good effect. Semantics-based preprocessing may be an effective scheme. RS images mosaic is a typical SDP where optimal mosaic line extraction is the crux. Lots of researches based on syntactic information are effective just for orthophoto maps. In this paper, an overall optimal mosaic line extraction scheme has been addressed for non-Ortho RS images. It is argued that there is no projection error in the projection datum fitted by Ground Control Points (GCPs), or regional main height surface which can be recognized in medium resolution RS images. Based on above reasons, the method suggests that GCPs collecting for precise geometrical correction should be on the main height surface, as well as the mosaic line extracting for RS images mosaic. Three sheets of CBERS CCD images of Taiyuan are taken as the experimental data. According to the afore-mentioned method, by collecting GCPs in wide riverbeds, all three sheets are rectified to an existing ETM+ mosaic image. And then, the central lines of wide riverbeds in the overlapping areas are extracted as the mosaic line. The experimental result indicates that this method can extract an overall optimal mosaic line and eliminate the visual texture seam-line effectively, even for non-Ortho RS images. It concludes that SDP based on semantic information can play a good role in spatial data applications.

  11. Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes.

    Science.gov (United States)

    Majtan, Tomas; Liu, Lu; Carpenter, John F; Kraus, Jan P

    2010-05-21

    Missense mutations represent the most common cause of many genetic diseases including cystathionine beta-synthase (CBS) deficiency. Many of these mutations result in misfolded proteins, which lack biological function. The presence of chemical chaperones can sometimes alleviate or even restore protein folding and activity of mutant proteins. We present the purification and characterization of eight CBS mutants expressed in the presence of chemical chaperones such as ethanol, dimethyl sulfoxide, or trimethylamine-N-oxide. Preliminary screening in Escherichia coli crude extracts showed that their presence during protein expression had a significant impact on the amount of recovered CBS protein, formation of tetramers, and catalytic activity. Subsequently, we purified eight CBS mutants to homogeneity (P49L, P78R, A114V, R125Q, E176K, P422L, I435T, and S466L). The tetrameric mutant enzymes fully saturated with heme had the same or higher specific activities than wild type CBS. Thermal stability measurements demonstrated that the purified mutants are equally or more thermostable than wild type CBS. The response to S-adenosyl-L-methionine stimulation or thermal activation varied. The lack of response of R125Q and E176K to both stimuli indicated that their specific conformations were unable to reach the activated state. Increased levels of molecular chaperones in crude extracts, particularly DnaJ, indicated a rather indirect effect of the chemical chaperones on folding of CBS mutants. In conclusion, the chemical chaperones present in the expression medium were able to fully restore the activity of eight CBS mutants by improving their protein folding. This finding could have direct implications for the development of a therapeutical approach to pyridoxine unresponsive homocystinuria.

  12. Prediction of CBS tidal evolution

    Science.gov (United States)

    Dryomova, G. N.

    The time series of basic processes, accompanying the tidal evolution of star components of Close Binary Systems (CBS) are predicted in the framework of evolutionary stellar models by Claret (2004). The series includes the apsidal motion period, timescale of synchronization of axial rotation of a star with the orbital revolution, the orbit circularization timescale, and the age. Data from the catalogues by Svechnikov & Perevozkina (1999) and by Torres, Andersen, Gimenez (2010) are used for testing the sensitivity of the numerical prediction algorithm.

  13. Fast kinetics of nucleotide binding to Clostridium perfringens family II pyrophosphatase containing CBS and DRTGG domains.

    Science.gov (United States)

    Jämsen, J; Baykov, A A; Lahti, R

    2012-02-01

    We earlier described CBS-pyrophosphatase of Moorella thermoacetica (mtCBS-PPase) as a novel phosphohydrolase that acquired a pair of nucleotide-binding CBS domains during evolution, thus endowing the protein with the capacity to be allosterically regulated by adenine nucleotides (Jämsen, J., Tuominen, H., Salminen, A., Belogurov, G. A., Magretova, N. N., Baykov, A. A., and Lahti, R. (2007) Biochem. J., 408, 327-333). We herein describe a more evolved type of CBS-pyrophosphatase from Clostridium perfringens (cpCBS-PPase) that additionally contains a DRTGG domain between the two CBS domains in the regulatory part. cpCBS-PPase retained the ability of mtCBS-PPase to be inhibited by micromolar concentrations of AMP and ADP and activated by ATP and was additionally activated by diadenosine polyphosphates (AP(n)A) with n > 2. Stopped-flow measurements using a fluorescent nucleotide analog, 2'(3')-O-(N-methylanthranoyl)-AMP, revealed that cpCBS-PPase interconverts through two different conformations with transit times on the millisecond scale upon nucleotide binding. The results suggest that the presence of the DRTGG domain affords greater flexibility to the regulatory part, allowing it to more rapidly undergo conformational changes in response to binding.

  14. Anatomical differences between CBS-corticobasal degeneration and CBS-Alzheimer's disease.

    Science.gov (United States)

    Josephs, Keith A; Whitwell, Jennifer L; Boeve, Bradley F; Knopman, David S; Petersen, Ronald C; Hu, William T; Parisi, Joseph E; Dickson, Dennis W; Jack, Clifford R

    2010-07-15

    We compare patterns of gray matter loss on MRI in subjects presenting as corticobasal syndrome (CBS) with Alzheimer disease pathology (CBS-AD) to those presenting as CBS with corticobasal degeneration pathology (CBS-CBD). Voxel-based morphometry was used to compare patterns of gray matter loss in pathologically confirmed CBS-AD subjects (n = 5) and CBS-CBD subjects (n = 6) to a group of healthy controls (n = 20), and to each other. Atlas based parcellation using the automated anatomic labeling atlas was also utilized in a region-of-interest analysis to account for laterality. The CBS-AD subjects were younger at the time of scan when compared with CBS-CBD subjects (median: 60 years vs. 69; P = 0.04). After adjusting for age at time of MRI scan, the CBS-AD subjects showed loss in posterior frontal, temporal, and superior and inferior parietal lobes, while CBS-CBD showed more focal loss predominantly in the posterior frontal lobes when compared with controls. In both CBS-AD and CBS-CBD groups, there was basal ganglia volume loss, yet relative sparing of hippocampi. On direct comparisons between the two subject groups, CBS-AD showed greater loss in both temporal and inferior parietal cortices than CBS-CBD. No regions showed greater loss in the CBS-CBD group compared to the CBS-AD group. These findings persisted when laterality was taken into account. In subjects presenting with CBS, prominent temporoparietal, especially posterior temporal and inferior parietal, atrophy may be a clue to the presence of underlying AD pathology.

  15. Draft Genome Sequence of Rubrivivax gelatinosus CBS

    Energy Technology Data Exchange (ETDEWEB)

    Hu, P. S.; Lang, J.; Wawrousek, K.; Yu, J. P.; Maness, P. C.; Chen, J.

    2012-06-01

    Rubrivivax gelatinosus CBS, a purple nonsulfur photosynthetic bacterium, can grow photosynthetically using CO and N{sub 2} as the sole carbon and nitrogen nutrients, respectively. R. gelatinosus CBS is of particular interest due to its ability to metabolize CO and yield H{sub 2}. We present the 5-Mb draft genome sequence of R. gelatinosus CBS with the goal of providing genetic insight into the metabolic properties of this bacterium.

  16. [Differential diagnosis of corticobasal syndrome (CBS) and its biomarkers].

    Science.gov (United States)

    Tokuda, Takahiko

    2013-01-01

    Corticobasal degeneration (CBD) was initially thought to represent a clinicopathological entity, but recent studies have shown its considerable clinicopathological heterogeneity. Thus, the constellation of the findings initially considered characteristic of CBD is now named "corticobasal syndrome" (CBS), while the term "CBD" is used for the histopathological disorder. Multiple phenotypes associated with CBD pathology and multiple diseases associated with CBS, the latter of which are called "CBD mimickers," make the correct diagnosis of these conditions on the basis of only clinical symptoms and signs difficult. Therefore, the development of specific biomarkers for diagnosing each causative disease of CBS is extremely important. Abnormal accumulations of the microtubule-associated tau protein are found in both neurons and glia, and CBD is thus considered one of the tauopathies. Although some studies have measured total and phosphorylated tau in the CSF of patients with CBS, their results are inconclusive. Disease-specific truncation and/or phosphorylation of tau protein and its fragments in the CSF would be a promising biomarker candidate; however, its effectiveness still needs to be confirmed by further studies. Progress in biomarker development to differentiate CBD from CBD mimickers has been slow, but it is essential to improve diagnostic accuracy and to develop disease-modifying therapies.

  17. [Expressions of CSE and CBS in the corpus cavernosum of spontaneous hypertensive rats].

    Science.gov (United States)

    Zhu, Xiu-Bo; Jiang, Jun; Jiang, Rui; Chen, Feng

    2014-01-01

    To investigate the expressions of cystathionine gamma-lyase (CSE) and cystathionine beta-synthase (CBS) in the corpus cavernosum of spontaneous hypertensive rats (SHR) and their relationship with erectile dysfunction. This study included 10 male SHRs and 10 healthy male Wistar-Kyoto (WKY) rats as controls, all aged 12 weeks. We applied a series of electric stimuli to the major pelvic ganglions of the rats, observed changes in the ratio of intracavernosal to mean arterial blood pressure (ICP/MAP), measured the levels of serum testosterone (T) and endogenous H2S, and determined the expressions of CSE and CBS in the corpus cavernosum by Western blot and immunohistochemistry. No obvious difference was found in the serum T level between the two groups. Compared with the WKY rats, the SHRs showed significant reduction in the ICP/MAP ratio, the contents of plasma H2S ([21.92 +/- 2.75] micromol/L vs [10.49 +/- 1.35] micromol/L, P CBS and CSE (P CBS were distributed mainly in the smooth muscle cells and vascular endothelial cells of the corpus cavernosum. The ICP/MAP ratio was highly positively correlated with the expressions of CSE (r = 0.977, P CBS (r = 0.955, P CBS in the corpus cavernosum, which might be related with hypertension-induced reduction of erectile function.

  18. Endogenous CBS-H2S Pathway Contributes to the Development of CCI-Induced Neuropathic Pain.

    Science.gov (United States)

    Gui, Yulong; Li, Aiyuan; Qiu, Bihui; Chen, Feng; Chen, Liang; Liu, Daming; Chen, Shuxian; Zhou, Wei; Zhou, Hong

    2016-06-01

    Studies showed a complex relationship between hydrogen sulfide (H2S) and neuropathic pain. In this study, the relationship between endogenous CBS-H2S pathway in L4-6 spinal cord and neuropathic pain was explored. A total of 163 adult Kunming mice were used in this study. CBS expression and H2S formation in L4-6 spinal cord were detected in the development of neuropathic pain firstly. Then, effect of AOAA, an CBS inhibitor, on treatment of neuropathic pain by chronic construction injury surgery (CCI) was detected. Pain thresholds and activation of NF-κB(p65), ERK1/2 and CREB were measured as biomarks of neuropathic pain. Results showed that CCI surgery significantly upregulated protein expression of CBS and H2S formation. Correlation analysis showed pain thresholds had negative relationships with protein expression of CBS and H2S formation. Treatment with AOAA, a CBS inhibitor, inhibited CCI-induced upregulation of CBS expression and H2S formation (P H2S pathway promoted the development of neuropathic pain. CBS-H2S pathway could be a promising target for treatment of neuropathic pain.

  19. Nitrotyrosinylation, remodeling and endothelial-myocyte uncoupling in iNOS, cystathionine beta synthase (CBS) knockouts and iNOS/CBS double knockout mice.

    Science.gov (United States)

    Kundu, Soumi; Kumar, Munish; Sen, Utpal; Mishra, Paras K; Tyagi, Neetu; Metreveli, Naira; Lominadze, David; Rodriguez, Walter; Tyagi, Suresh C

    2009-01-01

    Increased levels of homocysteine (Hcy), recognized as hyperhomocysteinemia (HHcy), were associated with cardiovascular diseases. There was controversy regarding the detrimental versus cardio protective role of inducible nitric oxide synthase (iNOS) in ischemic heart disease. The aim of this study was to test the hypothesis that the Hcy generated nitrotyrosine by inducing the endothelial nitric oxide synthase, causing endothelial-myocyte (E-M) coupling. To differentiate the role of iNOS versus constitutive nitric oxide synthase (eNOS and nNOS) in Hcy-mediated nitrotyrosine generation and matrix remodeling in cardiac dysfunction, left ventricular (LV) tissue was analyzed from cystathionine beta synthase (CBS) heterozygote knockout, iNOS homozygote knockout, CBS-/+/iNOS-/- double knockout, and wild-type (WT) mice. The levels of nitrotyrosine, MMP-2 and -9 (zymographic analysis), and fibrosis (by trichrome stain) were measured. The endothelial-myocyte function was determined in cardiac rings. In CBS-/+ mice, homocysteine was elevated and in iNOS-/- mice, nitric oxide was significantly reduced. The nitrotyrosine and matrix metalloproteinase-9 (MMP-9) levels were elevated in double knockout and CBS-/+ as compared to WT mice. Although MMP-2 levels were similar in CBS-/+, iNOS-/-, and CBS-/+/iNOS-/-, the levels were three- to fourfold higher than WT. The levels of collagen were similar in CBS-/+ and iNOS-/-, but they were threefold higher than WT. Interesting, the levels of collagen increased sixfold in double knockouts, compared to WT, suggesting synergism between high Hcy and lack of iNOS. Left ventricular hypertrophy was exaggerated in the iNOS-/- and double knockout, and mildly increased in the CBS-/+, compared to WT mice. The endothelial-dependent relaxation was attenuated to the same extent in the CBS-/+ and iNOS-/-, compared to WT, but it was robustly blunted in double knockouts. The results concluded that homocysteine generated nitrotyrosine in the vicinity of

  20. Determination of cystathionine beta-synthase activity in human plasma by LC-MS/MS: potential use in diagnosis of CBS deficiency.

    LENUS (Irish Health Repository)

    Krijt, Jakub

    2011-02-01

    Cystathionine β-synthase (CBS) deficiency is usually confirmed by assaying the enzyme activity in cultured skin fibroblasts. We investigated whether CBS is present in human plasma and whether determination of its activity in plasma could be used for diagnostic purposes. We developed an assay to measure CBS activity in 20 μL of plasma using a stable isotope substrate - 2,3,3-(2)H serine. The activity was determined by measurement of the product of enzyme reaction, 3,3-(2)H-cystathionine, using LC-MS\\/MS. The median enzyme activity in control plasma samples was 404 nmol\\/h\\/L (range 66-1,066; n = 57). In pyridoxine nonresponsive CBS deficient patients, the median plasma activity was 0 nmol\\/ho\\/L (range 0-9; n = 26), while in pyridoxine responsive patients the median activity was 16 nmol\\/hour\\/L (range 0-358; n = 28); this overlapped with the enzyme activity from control subject. The presence of CBS in human plasma was confirmed by an in silico search of the proteome database, and was further evidenced by the activation of CBS by S-adenosyl-L-methionine and pyridoxal 5\\'-phosphate, and by configuration of the detected reaction product, 3,3-(2)H-cystathionine, which was in agreement with the previously observed CBS reaction mechanism. We hypothesize that the CBS enzyme in plasma originates from liver cells, as the plasma CBS activities in patients with elevated liver aminotransferase activities were more than 30-fold increased. In this study, we have demonstrated that CBS is present in human plasma and that its catalytic activity is detectable by LC-MS\\/MS. CBS assay in human plasma brings new possibilities in the diagnosis of pyridoxine nonresponsive CBS deficiency.

  1. Determination of cystathionine beta-synthase activity in human plasma by LC-MS/MS: potential use in diagnosis of CBS deficiency.

    Science.gov (United States)

    Krijt, Jakub; Kopecká, Jana; Hnízda, Aleš; Moat, Stuart; Kluijtmans, Leo A J; Mayne, Philip; Kožich, Viktor

    2011-02-01

    Cystathionine β-synthase (CBS) deficiency is usually confirmed by assaying the enzyme activity in cultured skin fibroblasts. We investigated whether CBS is present in human plasma and whether determination of its activity in plasma could be used for diagnostic purposes. We developed an assay to measure CBS activity in 20 μL of plasma using a stable isotope substrate - 2,3,3-(2)H serine. The activity was determined by measurement of the product of enzyme reaction, 3,3-(2)H-cystathionine, using LC-MS/MS. The median enzyme activity in control plasma samples was 404 nmol/h/L (range 66-1,066; n = 57). In pyridoxine nonresponsive CBS deficient patients, the median plasma activity was 0 nmol/ho/L (range 0-9; n = 26), while in pyridoxine responsive patients the median activity was 16 nmol/hour/L (range 0-358; n = 28); this overlapped with the enzyme activity from control subject. The presence of CBS in human plasma was confirmed by an in silico search of the proteome database, and was further evidenced by the activation of CBS by S-adenosyl-L-methionine and pyridoxal 5'-phosphate, and by configuration of the detected reaction product, 3,3-(2)H-cystathionine, which was in agreement with the previously observed CBS reaction mechanism. We hypothesize that the CBS enzyme in plasma originates from liver cells, as the plasma CBS activities in patients with elevated liver aminotransferase activities were more than 30-fold increased. In this study, we have demonstrated that CBS is present in human plasma and that its catalytic activity is detectable by LC-MS/MS. CBS assay in human plasma brings new possibilities in the diagnosis of pyridoxine nonresponsive CBS deficiency.

  2. Assessment of the toxic potential of PCB congener 81 (3,4,4[prime],5-tetrachlorobiphenyl) to fish in relation to other non-ortho-substituted PCB congeners

    Energy Technology Data Exchange (ETDEWEB)

    Harris, G.E.; Kiparissis, Y.; Metcalfe, C.D. (Trent Univ., Peterborough, Ontario (Canada). Environmental and Resource Studies Program)

    1994-09-01

    PCB congener 81 (3,4,4[prime],5-tetrachlorobiphenyl) has been detected in fish tissues from various sites in North America. The embryotoxicity of this compound to medaka (Oryzias latipes) and the induction of hepatic aryl hydrocarbon hydroxylase (AHH) in rainbow trout (Oncorhynchus mykiss) were determined to assess the toxic potency of this compound relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and two other non-ortho-substituted PCB compounds, congener 77 (3,3[prime],4,4[prime]-tetrachlorobiphenyl) and congener 126 (3,3[prime],4,4[prime],5-pentachlorobiphenyl). The TCDD toxic equivalency factors (TEFs) estimated for congener 81 from two end points in the medaka embryotoxicity assay were 0.0014 (from mortality data) and 0.006 (from swim bladder inflation data). The TEF estimated for congener 81 from data on AHH induction in rainbow trout was 0.004. All TEFs were greater than those estimated for congener 77 but were less than the TEFs estimated for congener 126. On the basis of these toxicity data, it is suggested that this congener may contribute significantly to the toxic burden of planar halogenated aromatic hydrocarbon in fish.

  3. Energy Balance of the Netherlands. CBS versus IEA, Eurostat and UNFCCC; Energiebalans van Nederland. CBS versus IEA, Eurostat en UNFCCC

    Energy Technology Data Exchange (ETDEWEB)

    Segers, R.

    2010-03-15

    The physical energy system of the Netherlands is described in the so-called Energy balance of CBS (Statistics Netherlands). The statistical office of Europe (Eurostat) and the International Energy Agency (IEA) also publish an energy balance of the Netherlands. They use data provided to these organizations by CBS. The main lines of these balances are the same as the CBS balance. Nevertheless, there are quite a number of differences, despite the use of the same basic data. [Dutch] De fysieke energiehuishouding van Nederland wordt door het CBS beschreven in een zogenaamde energiebalans. Ook het statistische bureau van Europa (Eurostat) en het Internationaal Energieagentschap (IEA) publiceren een energiebalans van Nederland. Zij maken daarbij gebruik van gegevens die het CBS aan deze organisaties levert. Op hoofdlijnen lijken deze balansen op de CBS-balans. Ondanks het gebruik van dezelfde basisgegevens zijn er echter behoorlijk wat verschillen.

  4. CBS domains: Ligand binding sites and conformational variability.

    Science.gov (United States)

    Ereño-Orbea, June; Oyenarte, Iker; Martínez-Cruz, Luis Alfonso

    2013-12-01

    Cystathionine β-synthase (CBS) domains or CBS motifs are conserved structural domains that are present in thousands of non functionally-related proteins from all kingdoms of life. Their importance is underlined by the range of hereditary diseases associated with mutations in their amino acid sequence. CBS motifs associate in pairs referred to as Bateman modules. In contrast with initial assumptions, it is now well documented that CBS motifs and/or Bateman modules may suffer conformational changes upon binding of adenosine derivatives, metal ions or nucleic acids. The degree and direction of these structural changes depend on the type of ligand, the intrinsic features of the binding sites and the association manner of the Bateman modules. This review aims to provide a summary of the current knowledge on the structural basis of ligand recognition and on the structural effects caused by these ligands in CBS domain containing proteins. Copyright © 2013 Elsevier Inc. All rights reserved.

  5. Asteroseismology of the DBV star CBS 114

    Science.gov (United States)

    Chen, Yan-Hui

    2016-08-01

    Asteroseismology is a unique and powerful tool to investigate the internal structure of stars. CBS 114 is the sixth known pulsating DBV star. It was observed by Handler, Metcalfe, & Wood at the South African Astronomical Observatory over three weeks in 2001. Then, it was observed by Metcalfe et al. for seven nights (2004 Feb. 19-25) on the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory and seven nights (2004 Feb. 21-27) on the 2.1 m telescope at the McDonald Observatory. Totally two triplets, four doublets, and five singlets were identified. The frequency splitting values are very different, from 5.2 μHz to 11.9 μHz, which may reflect differential rotations. We evolve grids of white dwarf models by MESA. Cores, added with He/C envelopes, of those white dwarf models are inserted into WDEC to evolve grids of DBV star models. With those DBV star models, we calculate eigenperiods. Those calculated periods are used to fit observed periods. A best-fitting model is selected. The parameters are T eff = 25000 K, M * = 0.740 M ⊙ and log(M He/M *) = —4.5. With the relatively large stellar mass, the effective temperature is close to the previous spectroscopic result. In addition, kinetic energy distributions are calculated for the best-fitting model. We find that the observed modes with large frequency splitting values are fitted by the calculated modes with a large amount of kinetic energy distributed in the C/O core. After preliminary analysis, we suggest that the C/O core may rotate at least two times faster than the helium layer for CBS 114.

  6. X-ray imaging of differential vascular density in MMP-9-/-, PAR-1-/+, hyperhomocysteinemic (CBS-/+) and diabetic (Ins2-/+) mice.

    Science.gov (United States)

    Givvimani, S; Sen, U; Tyagi, N; Munjal, C; Tyagi, S C

    2011-02-01

    Although protease activated receptor-1 (PAR-1) and matrix metalloproteinase-9 (MMP-9) play significant role in vascular remodelling in hyperhomocysteinemia (HHcy due to cystathionine beta synthase deficiency, CBS-/+) and diabetes, mechanism remains nebulous. We hypothesized that differential vascular density and remodelling in different vascular beds in HHcy and diabetes were responsible for an adaptive metabolic homeostasis during the pathogenesis. To test this hypothesis, vascular density in mice lacking PAR-1, MMP-9, CBS and Insulin-2 gene mutant (Ins2-/+, Akita) was measured and compared with wild type (WT, C57BL/6J) mice. The vascular density was detected by x-ray angiography using KODAK 4000 MM image station, using barium sulphate as contrasting agent. The % vascular density in the hearts of WT, CBS-/+ (HHcy), MMP-9-/-, PAR-1-/+ and Ins2-/+ (type-1 diabetes) was 100 ± 2.8, 85 ± 3.3, 90 ± 3.3, 95 ± 3.8 and 73 ± 1.7, respectively. The vascular density in CBS-/+ and Akita hearts decreased while it was increased in lungs of CBS-/+ and MMP-9-/-.There was decreased vascular density in liver and kidney of Akita mice. Vascular density in brain, kidney and mesentery was decreased in CBS-/+ mice. These findings support the notation that metabolic derangement in diabetes and HHcy causes the chronic decline and/or rarefaction in vascular density.

  7. Influence of trophic ecology on the accumulation of dioxins and furans (PCDD/Fs), non-ortho polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) in Mediterranean gulls (Larus michahellis and L. audouinii): A three-isotope approach.

    Science.gov (United States)

    Roscales, Jose L; Vicente, Alba; Muñoz-Arnanz, Juan; Morales, Laura; Abad, Esteban; Aguirre, Jose I; Jiménez, Begoña

    2016-05-01

    The impact of pollution caused by severe anthropogenic pressure in the Mediterranean Sea, an important biodiversity hotspot, requires continuous research efforts. Sources of highly toxic chemicals such as Persistent Organic Pollutants (POPs) are misunderstood in representative Mediterranean species, which limits our capability to establish proper conservation strategies. In the present study, eggs of Audouin's and yellow-legged gulls (Larus audouinii and L. michahellis) were used to investigate the trophic sources, as measured by δ(13)C, δ(15)N, and δ(34)S, of legacy POPs, in particular, polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) and non-ortho polychlorinated biphenyls (no-PCBs), as well as recently-regulated POPs, e.g., polybrominated diphenyl ethers (PBDEs). Special attention was paid to the usefulness of rarely-explored δ(34)S ratios in explaining POP exposure in wildlife, and δ(34)S was the isotopic ratio that best explained POP variations among gulls in most cases, thus demonstrating its usefulness for understanding POP exposure in wildlife. Significant relationships between stable isotope signatures and POP concentrations revealed increasing levels of no-PCBs and low halogenated PCDD/Fs and PBDEs in Mediterranean gulls as the consumption of marine resources increases. In contrast, highly chlorinated and brominated congeners appeared to preferentially accumulate in gulls feeding primarily on refuse from dump sites and terrestrial food webs. The use of suitable dietary tracers in the study of POPs in yellow-legged gulls revealed the importance of dump sites as a source of POPs in Mediterranean seabirds, which has not previously been reported. In contrast, the preferential accumulation through marine food webs of low chlorinated PCCD/Fs and no-PCBs, which show the highest toxic equivalents factors (TEFs), led to a significantly greater toxicological concern in Audouin's as compared to yellow-legged gulls. Audouin's gull exposure to POPs appears

  8. The Child Behavior Scale (CBS) Revisited: A Longitudinal Evaluation of CBS Subscales with Children, Preadolescents, and Adolescents

    Science.gov (United States)

    Ladd, Gary W.; Herald-Brown, Sarah L.; Andrews, Rebecca K.

    2009-01-01

    The Child Behavior Scale (CBS) is a teacher-report instrument that was developed over a decade ago as an alternative to more costly methods for assessing children's behavior and peer relations in school contexts. This investigation was undertaken to clarify how well the CBS achieves its aims with a broader age spectrum of youth (6- to…

  9. Progression of brain atrophy in PSP and CBS over 6 months and 1 year.

    Science.gov (United States)

    Dutt, Shubir; Binney, Richard J; Heuer, Hilary W; Luong, Phi; Attygalle, Suneth; Bhatt, Priyanka; Marx, Gabe A; Elofson, Jonathan; Tartaglia, Maria C; Litvan, Irene; McGinnis, Scott M; Dickerson, Bradford C; Kornak, John; Waltzman, Dana; Voltarelli, Lisa; Schuff, Norbert; Rabinovici, Gil D; Kramer, Joel H; Jack, Clifford R; Miller, Bruce L; Rosen, Howard J; Boxer, Adam L

    2016-11-08

    To examine the utility and reliability of volumetric MRI in measuring disease progression in the 4 repeat tauopathies, progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS), to support clinical development of new tau-directed therapeutic agents. Six- and 12-month changes in regional MRI volumes and PSP Rating Scale scores were examined in 55 patients with PSP and 33 patients with CBS (78% amyloid PET negative) compared to 30 normal controls from a multicenter natural history study. Longitudinal voxel-based morphometric analyses identified patterns of volume loss, and region-of-interest analyses examined rates of volume loss in brainstem (midbrain, pons, superior cerebellar peduncle), cortical, and subcortical regions based on previously validated atlases. Results were compared to those in a replication cohort of 226 patients with PSP with MRI data from the AL-108-231 clinical trial. Patients with CBS exhibited greater baseline atrophy and greater longitudinal atrophy rates in cortical and basal ganglia regions than patients with PSP; however, midbrain and pontine atrophy rates were similar. Voxel-wise analyses showed distinct patterns of regional longitudinal atrophy in each group as compared to normal controls. The midbrain/pons volumetric ratio differed between diagnoses but remained stable over time. In both patient groups, brainstem atrophy rates were correlated with disease progression measured using the PSP Rating Scale. Volume loss is quantifiable over a period of 6 months in CBS and PSP. Future clinical trials may be able to combine CBS and PSP to measure therapeutic effects. © 2016 American Academy of Neurology.

  10. Asteroseismology of the DBV star CBS 114

    CERN Document Server

    Chen, Yanhui

    2016-01-01

    Asteroseismology is an unique and powerful tool to detect the internal structure of stars. CBS 114 is the sixth known pulsating DBV star. It was observed by Handler, Metcalfe, \\& Wood at South African Astronomical Observatory over 3 weeks in 2001. Then, it was observed by Metcalfe et al. at Bohyunsan Optical Astronomy Observatory and McDonald Observatory respectively for 7 nights in 2004. Totally 2 triplets, 4 doublets, and 5 singlets were identified. The frequency splitting values are very different, from 5.2 $\\mu$Hz to 11.9 $\\mu$Hz, which may reflect differential rotations. We evolve grids of white dwarf models by \\texttt{MESA}. Cores, added with He/C envelopes, of those white dwarf models are inserted into \\texttt{WDEC} to evolve grids of DBV star models. With those DBV star models, we calculate eigenperiods. Those calculated periods are used to fit observed periods. A best-fitting model is selected. The parameters are $T_{eff}$ = 25000 K, $M_{*}$ = 0.740 $M_{\\odot}$, and log($M_{He}/M_{*}$) = -4.5. Wi...

  11. Evaluation of CBS 600 carburized steel as a gear material

    Science.gov (United States)

    Townsend, D. P.; Parker, R. J.; Zaretsky, E. V.

    1979-01-01

    Gear endurance tests were conducted with one lot of consumable-electrode vacuum-melted (CVM) AISI 9310 gears and one lot of air-melt CBS 600 gears. The gears were 8 pitch with a pitch diameter of 8.89 centimeters (3.5 in.). Bench-type rolling-element fatigue tests were also conducted with one lot of CVM AISI 9310, three lots of CVM CBS 600, and one of air-melt CBS 600 material. The rolling-element bars were 0.952 centimeter (0.375 in.) in diameter. The CBS 600 material exhibited pitting fatigue lives in both rolling-element specimens and gears at least equivalent to that of CVM AISI 9310. Tooth fracture failure occurred with the CBS 600 gears after overrunning a fatigue spall, but it did not occur with the CVM AISI 9310 gears. Tooth fracture in the CBS 600 was attributed to excessive carbon content in the case, excessive case depth, and a higher than normal core hardness.

  12. How reliable are gas-phase proton affinity values of small carbanions? A comparison of experimental data with values calculated using Gaussian-3 and CBS compound methods

    Science.gov (United States)

    Danikiewicz, Witold

    2009-08-01

    Gas-phase proton affinities (PA) of a series of 25 small, aliphatic carbanions were computed using different Gaussian-3 methods: G3, G3(B3LYP), G3(MP2) and G3(MP2, B3LYP) and Complete Basis Set Extrapolation methods: CBS-4M, CBS-Q, CBS-QB3, and CBS-APNO. The results were compared with critically selected experimental data. The analysis of the results shows that for the majority of the studied molecules all compound methods (Gaussian-3 and CBS), except for CBS-4M, give comparable results, which differ no more than +/-2 kcal mol-1 from the experimental data. Taking into account the calculation time, G3(MP2) and G3(MP2, B3LYP) methods offer the best compromise between accuracy and computational cost. As an additional proof, the results obtained by these two methods were compared with the values obtained using CCSD(T) ab initio method with large basis set. It was found also that some of the published experimental data are erroneous and should be corrected. The results described in this work show that for the majority of the studied compounds PA values calculated using compound methods can be used with the same or even higher confidence as the experimental ones because even the largest differences between Gaussian-3 and CBS methods listed above are still comparable with the accuracy of the typical PA measurements.

  13. Benchmarking Compound Methods (CBS-QB3, CBS-APNO, G3, G4, W1BD) against the Active Thermochemical Tables: A Litmus Test for Cost-Effective Molecular Formation Enthalpies.

    Science.gov (United States)

    Simmie, John M; Somers, Kieran P

    2015-07-16

    The theoretical atomization energies of some 45 CxHyOz molecules present in the Active Thermochemical Tables compilation and of particular interest to the combustion chemistry community have been computed using five composite model chemistries as titled. The species contain between 1-8 "heavy" atoms, and a few are conformationally diverse with up to nine conformers. The enthalpies of formation at 0 and 298.15 K are then derived via the atomization method and compared against the recommended values. In general, there is very good agreement between our averaged computed values and those in the ATcT; those for 1,3-cyclopentadiene exceptionally differ considerably, and we show from isodesmic reactions that the true value for 1,3-cyclopentadiene is closer to 134 kJ mol(-1) than the reported 101 kJ mol(-1). If one is restricted to using a single method, statistical measures indicate that the best methods are in the rank order G3 ≈ G4 > W1BD > CBS-APNO > CBS-QB3. The CBS-x methods do on average predict ΔfH(⊖)(298.15 K) within ≈5 kJ mol(-1) but are prone to occasional lapses. There are statistical advantages to be gained from using a number of methods in tandem, and all possible combinations have been tested. We find that the average formation enthalpy coming from using CBS-APNO/G4, CBS-APNO/G3, and G3/G4 show lower mean signed and mean unsigned errors, and lower standard and root-mean-squared deviations, than any of these methods in isolation. Combining these methods also leads to the added benefit of providing an uncertainty rooted in the chemical species under investigation. In general, CBS-APNO and W1BD tend to underestimate the formation enthalpies of target species, whereas CBS-QB3, G3, and G4 have a tendency to overestimate the same. Thus, combining CBS-APNO with a G3/G4 combination leads to an improvement in all statistical measures of accuracy and precision, predicting the ATcT values to within 0.14 ± 4.21 kJ mol(-1), thus rivalling "chemical accuracy" (

  14. A case of homocystinuria due to CBS gene mutations revealed by cerebral venous thrombosis.

    Science.gov (United States)

    Sarov, Mariana; Not, Adeline; de Baulny, Hélène Ogier; Masnou, Pascal; Vahedi, Katayoun; Bousser, Marie-Germaine; Denier, Christian

    2014-01-15

    Homocystinuria caused by cystathionine beta synthase (CBS) deficiency is most often diagnosed in childhood and has a variable expressivity. The most frequent abnormalities include intellectual disability, ectopia lentis, myopia, skeletal abnormalities or thromboembolism. To report a case of homocystinuria unraveled by cerebral venous thrombosis (CVT). A 17 year old female was admitted in our department of neurology for subacute headache and presented seizures in the emergency room. Cerebral imaging revealed CVT. Severe hyperhomocysteinemia was found and led to the diagnosis of homocystinuria due to composite heterozygous mutations in the CBS gene. Further investigations disclosed lens subluxation in association with myopia, mild scoliosis and osteopenia. The patient was treated by heparin followed by warfarin, vitamin therapy and dietary methionine restriction. Total homocysteine and methionine levels became normal in a few weeks and the patient had a complete recovery. In patients with CVT, plasma total homocysteine measurement as part of the etiologic work up may reveal severe hyperhomocysteinemia due to CBS or remethylation defects that require specific treatment and management including perhaps protein-restricted diet and/or vitamin therapy for life. © 2013.

  15. CBS-dekan: Er godt nok godt nok?

    DEFF Research Database (Denmark)

    Møllgaard, Peter

    2017-01-01

    En evaluering af ph.d.-uddannelserne i Danmark viser, at det går godt nok. Skal de være bedre, kræver det mere tid, flere ph.d.-studerende og strukturerede forløb, skriver Peter Møllgaard, forskningsdekan på CBS......En evaluering af ph.d.-uddannelserne i Danmark viser, at det går godt nok. Skal de være bedre, kræver det mere tid, flere ph.d.-studerende og strukturerede forløb, skriver Peter Møllgaard, forskningsdekan på CBS...

  16. Lewis Acids as Activators in CBS-Catalysed Diels-Alder Reactions: Distortion Induced Lewis Acidity Enhancement of SnCl4.

    Science.gov (United States)

    Nödling, Alexander R; Möckel, Robert; Tonner, Ralf; Hilt, Gerhard

    2016-09-05

    The effect of several Lewis acids on the CBS catalyst (named after Corey, Bakshi and Shibata) was investigated in this study. While (2) H NMR spectroscopic measurements served as gauge for the activation capability of the Lewis acids, in situ FT-IR spectroscopy was employed to assess the catalytic activity of the Lewis acid oxazaborolidine complexes. A correlation was found between the Δδ((2) H) values and rate constants kDA , which indicates a direct translation of Lewis acidity into reactivity of the Lewis acid-CBS complexes. Unexpectedly, a significant deviation was found for SnCl4 as Lewis acid. The SnCl4 -CBS adduct was much more reactive than the Δδ((2) H) values predicted and gave similar reaction rates to those observed for the prominent AlBr3 -CBS adduct. To rationalize these results, quantum mechanical calculations were performed. The frontier molecular orbital approach was applied and a good correlation between the LUMO energies of the Lewis acid-CBS-naphthoquinone adducts and kDA could be found. For the SnCl4 -CBS-naphthoquinone adduct an unusual distortion was observed leading to an enhanced Lewis acidity. Energy decomposition analysis with natural orbitals for chemical valence (EDA-NOCV) calculations revealed the relevant interactions and activation mode of SnCl4 as Lewis acid in Diels-Alder reactions. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. FUS and TDP43 genetic variability in FTD and CBS.

    Science.gov (United States)

    Huey, Edward D; Ferrari, Raffaele; Moreno, Jorge H; Jensen, Christopher; Morris, Christopher M; Potocnik, Felix; Kalaria, Rajesh N; Tierney, Michael; Wassermann, Eric M; Hardy, John; Grafman, Jordan; Momeni, Parastoo

    2012-05-01

    This study aimed to evaluate genetic variability in the FUS and TDP-43 genes, known to be mainly associated with amyotrophic lateral sclerosis (ALS), in patients with the diagnoses of frontotemporal lobar degeneration (FTLD) and corticobasal syndrome (CBS). We screened the DNA of 228 patients for all the exons and flanking introns of FUS and TDP-43 genes. We identified 2 novel heterozygous missense mutations in FUS: P106L (g.22508384C>T) in a patient with behavioral variant frontotemporal dementia (bvFTD) and Q179H in several members of a family with behavioral variant FTD. We also identified the N267S mutation in TDP-43 in a CBS patient, previously only reported in 1 ALS family and 1 FTD patient. Additionally, we identified 2 previously reported heterozygous insertion and deletion mutations in Exon 5 of FUS; Gly174-Gly175 del GG (g. 4180-4185 delGAGGTG) in an FTD patient and Gly175-Gly176 ins GG (g. 4185-4186 insGAGGTG) in a patient with diagnosis of CBS. Not least, we have found a series of variants in FUS also in neurologically normal controls. In summary, we report that genetic variability in FUS and TDP-43 encompasses a wide range of phenotypes (including ALS, FTD, and CBS) and that there is substantial genetic variability in FUS gene in neurologically normal controls. Copyright © 2012 Elsevier Inc. All rights reserved.

  18. Penentuan Bilangan Iodin pada Cocoa Butter Substitute (CBS)

    OpenAIRE

    Situmorang, Dhesy Phatiarma

    2015-01-01

    Determination has been made of Iodine Value with titration methods in some Cocoa Butter Substitute (CBS) in PT. Palmcoco Laboratories, Medan. Results obtained from the average level of iodine value Cocoa Butter Substitute derives from Medan is 0,21 gr I2/100gr and from Kuala Tanjung is 0,29 gr I2/100gr. 112401022

  19. Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation.

    Science.gov (United States)

    Krahulec, Stefan; Kratzer, Regina; Longus, Karin; Nidetzky, Bernd

    2012-03-01

    The various strains of Scheffersomyces stipitis (Pichia stipitis) differ substantially with respect to their ability to ferment xylose into ethanol. Two P. stipitis strains CBS 5773 and CBS 6054 have been most often used in literature but comparison of their performance in xylose fermentation under identical conditions has not been reported so far. Conversion of xylose (22 g/L) by each of these P. stipitis strain was analyzed under anaerobic and microaerobic conditions. Ethanol yields of ∼0.41 g/g were independent of strain and conditions used. Glycerol and acetate were formed in constant yields of 0.006 g/g and 0.02 g/g, respectively. Xylitol formation decreased from ∼0.08 g/g to ∼0.05 g/g upon switch from anaerobic to microaerobic conditions. Specific activities of enzymes of the two-step oxidoreductive xylose conversion pathway (xylose reductase and xylitol dehydrogenase) matched for both strains within limits of error. When xylose was offered at 76 g/L under microaerobic reaction conditions, ethanol yields were still high (0.37-0.39 g/g) for both strains even though the xylitol yields (0.12-0.13 g/g) were increased as compared to the conditions of low xylose concentration. P. stipitis strains CBS 5773 and CBS 6054 are therefore identical by the criteria selected and show useful performance during conversion of xylose into ethanol, irrespective of the supply of oxygen.

  20. Exploring the molecular forces within and between CbsA S-layer proteins using single molecule force spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Verbelen, Claire [Unite de chimie des interfaces, Universite catholique de Louvain, Croix du Sud 2/18, B-1348 Louvain-la-Neuve (Belgium); Antikainen, Jenni; Korhonen, Timo K. [General Microbiology, Faculty of Biosciences, University of Helsinki, Helsinki, Fin-00014 (Finland); Dufrene, Yves F. [Unite de chimie des interfaces, Universite catholique de Louvain, Croix du Sud 2/18, B-1348 Louvain-la-Neuve (Belgium)], E-mail: dufrene@cifa.ucl.ac.be

    2007-10-15

    We used single molecule atomic force microscopy (AFM) to gain insight into the molecular forces driving the folding and assembly of the S-layer protein CbsA. Force curves recorded between tips and supports modified with CbsA proteins showed sawtooth patterns with multiple force peaks of 58{+-}26 pN that we attribute to the unfolding of {alpha}-helices, in agreement with earlier secondary structure predictions. The average unfolding force increased with the pulling speed but was independent on the interaction time. Force curves obtained for CbsA peptides truncated in their C-terminal region showed similar periodic features, except that fewer force peaks were seen. Furthermore, the average unfolding force was 83{+-}45 pN, suggesting the domains were more stable. By contrast, cationic peptides truncated in their N-terminal region showed single force peaks of 366{+-}149 pN, presumably reflecting intermolecular electrostatic bridges rather than unfolding events. Interestingly, these large intermolecular forces increased not only with pulling speed but also with interaction time. We expect that the intra- and intermolecular forces measured here may play a significant role in controlling the stability and assembly of the CbsA protein.

  1. [Distribution Characteristics and Potential Risk of CBs in Aquatic Organisms from Typical Epidemic Areas of Schistosomiasis Prevalence].

    Science.gov (United States)

    Li, Kun; Zhao, Gao-feng; Zhou, Huai-dong; Zhao, Jian; Zhang, Pan-wei; Liu, Qiao-na; Wang, Xing-xun; Liu, Xiao-ru

    2015-10-01

    Eleven mollusca samples and 32 fish samples were collected from typical epidemic areas of schistosomiasis prevalence. The contents of 12 CB congeners in aquatic organism samples were measured using the GC/MS technique. The results indicated that 1,3-DIC, 1,4-DIC, 1,2-DIC and HCB were the most predominant CB congeners in the samples from the studying area. The geometric average of CBs in muscle of mollusca was 11 947 ng x g(-1) lipid weight. The summarized concentration of CBs in fish muscle ranged from 1 851 to 8 159 ng x g(-1) lipid weight. The highest concentration of CBs sum was detected in the catfish, while the lowest one was found in the crucian carp. The concentrations of CBs in fish samples were much higher than those reported in other countries, while in mollusca samples were at the same level. The estimated cumulative cancerigenic risks for the local residents consumed the aquatic organisms from the studying area were 1.49 x 10(-7) and 3.73-21.1 x 10(-7), respectively for mollusca and fish, both of which were acceptable.

  2. Effect of S-adenosyl-L-methionine (SAM), an allosteric activator of cystathionine-β-synthase (CBS) on colorectal cancer cell proliferation and bioenergetics in vitro.

    Science.gov (United States)

    Módis, Katalin; Coletta, Ciro; Asimakopoulou, Antonia; Szczesny, Bartosz; Chao, Celia; Papapetropoulos, Andreas; Hellmich, Mark R; Szabo, Csaba

    2014-09-15

    Recent data show that colon cancer cells selectively overexpress cystathionine-β-synthase (CBS), which produces hydrogen sulfide (H2S), to maintain cellular bioenergetics, support tumor growth and stimulate angiogenesis and vasorelaxation in the tumor microenvironment. The purpose of the current study was to investigate the effect of the allosteric CBS activator S-adenosyl-L-methionine (SAM) on the proliferation and bioenergetics of the CBS-expressing colon cancer cell line HCT116. The non-transformed, non-tumorigenic colon epithelial cell line NCM356 was used as control. For assessment of cell proliferation, the xCELLigence system was used. Bioenergetic function was measured by Extracellular Flux Analysis. Experiments using human recombinant CBS or HCT116 homogenates complemented the cell-based studies. SAM markedly enhanced CBS-mediated H2S production in vitro, especially when a combination of cysteine and homocysteine was used as substrates. Addition of SAM (0.1-3 mM) to HCT116 cells induced a concentration-dependent increase H2S production. SAM exerted time- and concentration-dependent modulatory effects on cell proliferation. At 0.1-1 mM SAM increased HCT116 proliferation between 0 and 12 h, while the highest SAM concentration (3 mM) inhibited proliferation. Over a longer time period (12-24 h), only the lowest concentration of SAM used (0.1 mM) stimulated cell proliferation; higher SAM concentrations produced a concentration-dependent inhibition. The short-term stimulatory effects of SAM were attenuated by the CBS inhibitor aminooxyacetic acid (AOAA) or by stable silencing of CBS. In contrast, the inhibitory effects of SAM on cell proliferation was unaffected by CBS inhibition or CBS silencing. In contrast to HCT116 cells, the lower rate of proliferation of the low-CBS expressor NCM356 cells was unaffected by SAM. Short-term (1 h) exposure of HCT116 cells to SAM induced a concentration-dependent increase in oxygen consumption and bioenergetic function at 0

  3. Growth of Saccharomyces cerevisiae CBS 426 on mixtures of glucose and succinic acid: a model

    Energy Technology Data Exchange (ETDEWEB)

    Bonnet, J.A.B.A.F.; Koellmann, C.J.W.; Dekkers-de Kok, H.E.; Roels, J.A.

    1984-03-01

    Saccharomyces cerevisiae CBS 426 was grown in continuous culture in a defined medium with a mixture of glucose and succinic acid as the carbon source. Growth on succinic acid was possible after long adaptation periods. The flows of glucose, succinic acid, oxygen, carbon dioxide, and biomass to and from the system were measured. It proved necessary to expand our previous model to accommodate the active transport of succinic acid by the cell. The values found for the efficiency of the oxidative phosphorylation (PIO) and the amount of ATP needed for production of biomass from monomers gave the same values as found for substrate mixtures taken up passively. (Refs. 13).

  4. Et langt liv med styreformer på CBS

    DEFF Research Database (Denmark)

    Mouritsen, Johannes

    2016-01-01

    Artiklens forfatter, Johannes Mouritsen, fortæller med undertitlerne ’fra Røffel til Revolver’ og ’fra Hjerne til Hjerte til Hjerne’ om sine 53 år på CBS. Dette er første del af hans krønike, der handler om tiden med Professorvældet fra 1963-1972.......Artiklens forfatter, Johannes Mouritsen, fortæller med undertitlerne ’fra Røffel til Revolver’ og ’fra Hjerne til Hjerte til Hjerne’ om sine 53 år på CBS. Dette er første del af hans krønike, der handler om tiden med Professorvældet fra 1963-1972....

  5. Direct weak localization signature with ultracold atoms: the CBS revival

    Science.gov (United States)

    Josse, Vincent

    2016-05-01

    Ultracold atomic systems in presence of disorder have attracted a lot of interest over the past decade, in particular to study the physics of Anderson localization (AL) in a renewed perspective. Landmark experiments have been demonstrated, in 1D and 3D geometries. However many challenges remain and new ideas have emerged, as for instance the search for original signatures of Anderson localization in momentum space. Here I will describe our progresses along that line where a weak localization effect has been directly observed, i.e. the Coherent Backscattering (CBS) phenomenon. In particular I will report on the recent observation of suppression and revival of CBS when a controlled dephasing kick is applied to the system. This observation demonstrates a novel and general method, introduced by T. Micklitz and coworkers, to study probe phase coherence in disordered systems by manipulating time reversal symmetry.

  6. CBS/ICE program user's guide

    Energy Technology Data Exchange (ETDEWEB)

    Silver, S.C.; Jones, J.W.; Peterson, J.L.; Milbitz, A.; Hunn, B.D.

    1988-06-01

    A wide variety of tools are available for the energy analysis of buildings. These range in complexity from manual degree-day-based approaches to bin methods (often performed on microcomputer spreadsheets) to sophisticated hourly simulation codes that typically run on mainframe computers. Few of these programs have the detailed algorithms necessary to model the different HVAC system and central plant configurations found in many commercial buildings. This report documents the development of an hourly computer-simulation program for modeling static (ice-on-coil) ice-storage systems to provide thermal storage in HVAC systems applications. The program, called CBS/ICE, is structured as part of the DOE-2/CBS building energy-analysis computer program, but can be run in a stand alone mode. It simulates, in detail, the seasonal energy use and peak-demand performance of HVAC systems incorporating ice storage for use by ice-storage-system designers.

  7. The CBS domain: a protein module with an emerging prominent role in regulation.

    Science.gov (United States)

    Baykov, Alexander A; Tuominen, Heidi K; Lahti, Reijo

    2011-11-18

    Regulatory CBS (cystathionine β-synthase) domains exist as two or four tandem copies in thousands of cytosolic and membrane-associated proteins from all kingdoms of life. Mutations in the CBS domains of human enzymes and membrane channels are associated with an array of hereditary diseases. Four CBS domains encoded within a single polypeptide or two identical polypeptides (each having a pair of CBS domains at the subunit interface) form a highly conserved disk-like structure. CBS domains act as autoinhibitory regulatory units in some proteins and activate or further inhibit protein function upon binding to adenosine nucleotides (AMP, ADP, ATP, S-adenosyl methionine, NAD, diadenosine polyphosphates). As a result of the differential effects of the nucleotides, CBS domain-containing proteins can sense cell energy levels. Significant conformational changes are induced in CBS domains by bound ligands, highlighting the structural basis for their effects.

  8. An efficient extrapolation to the (T)/CBS limit

    Science.gov (United States)

    Ranasinghe, Duminda S.; Barnes, Ericka C.

    2014-05-01

    We extrapolate to the perturbative triples (T)/complete basis set (CBS) limit using double ζ basis sets without polarization functions (Wesleyan-1-Triples-2ζ or "Wes1T-2Z") and triple ζ basis sets with a single level of polarization functions (Wesleyan-1-Triples-3ζ or "Wes1T-3Z"). These basis sets were optimized for 102 species representing the first two rows of the Periodic Table. The species include the entire set of neutral atoms, positive and negative atomic ions, as well as several homonuclear diatomic molecules, hydrides, rare gas dimers, polar molecules, such as oxides and fluorides, and a few transition states. The extrapolated Wes1T-(2,3)Z triples energies agree with (T)/CBS benchmarks to within ±0.65 mEh, while the rms deviations of comparable model chemistries W1, CBS-APNO, and CBS-QB3 for the same test set are ±0.23 mEh, ±2.37 mEh, and ±5.80 mEh, respectively. The Wes1T-(2,3)Z triples calculation time for the largest hydrocarbon in the G2/97 test set, C6H5Me+, is reduced by a factor of 25 when compared to W1. The cost-effectiveness of the Wes1T-(2,3)Z extrapolation validates the usefulness of the Wes1T-2Z and Wes1T-3Z basis sets which are now available for a more efficient extrapolation of the (T) component of any composite model chemistry.

  9. An efficient extrapolation to the (T)/CBS limit.

    Science.gov (United States)

    Ranasinghe, Duminda S; Barnes, Ericka C

    2014-05-14

    We extrapolate to the perturbative triples (T)/complete basis set (CBS) limit using double ζ basis sets without polarization functions (Wesleyan-1-Triples-2ζ or "Wes1T-2Z") and triple ζ basis sets with a single level of polarization functions (Wesleyan-1-Triples-3ζ or "Wes1T-3Z"). These basis sets were optimized for 102 species representing the first two rows of the Periodic Table. The species include the entire set of neutral atoms, positive and negative atomic ions, as well as several homonuclear diatomic molecules, hydrides, rare gas dimers, polar molecules, such as oxides and fluorides, and a few transition states. The extrapolated Wes1T-(2,3)Z triples energies agree with (T)/CBS benchmarks to within ±0.65 mEh, while the rms deviations of comparable model chemistries W1, CBS-APNO, and CBS-QB3 for the same test set are ±0.23 mEh, ±2.37 mEh, and ±5.80 mEh, respectively. The Wes1T-(2,3)Z triples calculation time for the largest hydrocarbon in the G2/97 test set, C6H5Me(+), is reduced by a factor of 25 when compared to W1. The cost-effectiveness of the Wes1T-(2,3)Z extrapolation validates the usefulness of the Wes1T-2Z and Wes1T-3Z basis sets which are now available for a more efficient extrapolation of the (T) component of any composite model chemistry.

  10. An efficient extrapolation to the (T)/CBS limit

    Energy Technology Data Exchange (ETDEWEB)

    Ranasinghe, Duminda S. [Hall-Atwater Laboratories of Chemistry, Wesleyan University, Middletown, Connecticut 06459-0180 (United States); Barnes, Ericka C., E-mail: barnese8@southernct.edu [Department of Chemistry, Southern Connecticut State University, 501 Crescent Street, New Haven, Connecticut 06515-1355 (United States)

    2014-05-14

    We extrapolate to the perturbative triples (T)/complete basis set (CBS) limit using double ζ basis sets without polarization functions (Wesleyan-1-Triples-2ζ or “Wes1T-2Z”) and triple ζ basis sets with a single level of polarization functions (Wesleyan-1-Triples-3ζ or “Wes1T-3Z”). These basis sets were optimized for 102 species representing the first two rows of the Periodic Table. The species include the entire set of neutral atoms, positive and negative atomic ions, as well as several homonuclear diatomic molecules, hydrides, rare gas dimers, polar molecules, such as oxides and fluorides, and a few transition states. The extrapolated Wes1T-(2,3)Z triples energies agree with (T)/CBS benchmarks to within ±0.65 mE{sub h}, while the rms deviations of comparable model chemistries W1, CBS-APNO, and CBS-QB3 for the same test set are ±0.23 mE{sub h}, ±2.37 mE{sub h}, and ±5.80 mE{sub h}, respectively. The Wes1T-(2,3)Z triples calculation time for the largest hydrocarbon in the G2/97 test set, C{sub 6}H{sub 5}Me{sup +}, is reduced by a factor of 25 when compared to W1. The cost-effectiveness of the Wes1T-(2,3)Z extrapolation validates the usefulness of the Wes1T-2Z and Wes1T-3Z basis sets which are now available for a more efficient extrapolation of the (T) component of any composite model chemistry.

  11. [Expressions of CSE and CBS in the penile corpus cavernosum of hyperglycemia rats and their implications].

    Science.gov (United States)

    Huang, Yi-Ming; Xia, Ji-Yi; Jiang, Rui

    2014-04-01

    To investigate the impact of hyperglycemia on the hydrogen sulfide (H2S) signaling pathway in rat penile tissue and its relationship with erectile function. Twenty healthy male Sprague Dawley (SD) rats aged 8 weeks were randomly divided into groups A (4-week healthy control), B (4-week diabetes mellitus model), C (6-week healthy control) and D (6-week diabetes mellitus model). The rats in groups B and D were injected intraperitoneally with streptozotocin at 50 mg/kg to induce diabetes mellitus, while those in groups A and C with the same volume of normal saline. The animals were killed at 4 (groups A and B) and 6 weeks (groups C and D) after treatment for measurement of the maximal intracavernous pressure/mean arterial blood pressure (ICP(max)/MAP) by electrostimulation, determination of the H2S concentration in the plasma and penile tissue, and detection of the expressions of cystathionine-beta-synthetase (CBS) and cystathionine-gamma-lyase (CSE) in the penile corpus cavernosum by immunohisto- chemistry and Western blot. With electrostimulation of the pelvic ganglia at 5V and 7 V, ICP(max)/MAP was significantly reduced in groups B (0.19 +/- 0.03 and 0.29 +/- 0.04) and D (0.14 +/- 0.04 and 0.25 +/- 0.04) as compared with A (0.46 +/- 0.07 and 0.68 +/- 0.09) and C (0.43 +/- 0.07 and 0.65 +/- 0.16) (P 0.05) or between C and D ([470.44 +/- 209.28] ng/dl vs [297.01 +/- 96.58] ng/dl, P > 0.05). Groups B and D showed remarkable reduction in the H2S concentration (P CBS and CSE (P CBS and CSE expressions were even more significantly decreased in D than in B (P CBS and CSE in the penile corpus cavernosum of the diabetic rats suggested that the H2S signaling pathway might be involved in hyperglycemia-induced erectile dysfunction.

  12. beta-xylanase produced by Aureobasidium pullulans CBS 58475

    Energy Technology Data Exchange (ETDEWEB)

    Dobberstein, J.; Emeis, C.C. (Technische Hochschule Aachen (Germany, F.R.). Inst. und Lehrstuhl fuer Biologie 4 (Mikrobiologie))

    1989-12-01

    Aureobasidium pullulans CBS 58475 produced beta-xylanase with an activity of 5 units/ml culture filtrate. Xylose, xylan and complex substrates containing xylose served as strong inducers. Purification of the enzyme was achieved by two sets of gel permeation chromatography. The enzyme has an pH optimum at 4.25 and a temperature optimum at 60deg C. At slightly acid pH and temperatures up to 60deg C beta-xylanase showed good stability. The analysis of cleavage products classified the beta-xylanase as an endoenzyme. Together with an endopolygalacturonase, the beta-xylanase enhanced the maceration of carrots compared to endopolygalacturonase alone. (orig.).

  13. CBS - A program for close binary system light curve analysis

    Science.gov (United States)

    Solmi, L.; Galli, M.

    CBS is a new program for binary system light curve analysis, it generates synthetic light curves for a binary system, accounting for eclipses, tidal distortion, limb darkening, gravity darkening and reflection; it is also possible to compute the light contribution and eclipses of an accretion disk. The bolometric light curve is generated, as well as curves for the U,B,V,R,I colour bands. In the following we give a brief description of the first version of the program and show some preliminary results.

  14. CCSD(T)/CBS atomic and molecular benchmarks for H through Ar

    Science.gov (United States)

    Ranasinghe, Duminda S.; Petersson, George A.

    2013-04-01

    We extrapolate to the coupled cluster single and double excitation and the perturbative triples (CCSD(T))/complete basis set (CBS) limit with a sequence of optimized n-tuple-ζ augmented polarization augmented (nZaPa) basis sets (n = 4, 5, 6, and 7) for 115 species representing the first two rows of the Periodic Table. The species include the entire set of atoms, positive and negative atomic ions, homonuclear diatomic molecules, and hydrides. The benchmark set also includes the rare gas dimers, polar molecules such as oxides and fluorides, and a few transition states for chemical reactions. The CCSD correlation energies agree with available CCSD-F12b/3C(FIX) values to within ±0.18 mEh root-mean-square (rms) deviation. The (T) components agree to within ±0.10 mEh and the total CCSD(T) correlation energies to within ±0.26 mEh or 0.1% rms deviation, which is probably the better measure, since the largest deviation is 0.43 mEh or 0.13%. These CBS limits can now be used as benchmarks to calibrate more approximate calculations using smaller basis sets. The sequence of basis sets provides data on convergence patterns for each component of the correlation energy.

  15. The adiabatic electron affinities (EAs) for the heteroatomic molecule SO 4: An MP2/CBS study

    Science.gov (United States)

    Zheng, Wenxu; Lau, Kai-Chung; Wong, Ning-Bew; Li, Wai-Kee

    2009-01-01

    An MP2/CBS approach has been proposed to calculate the adiabatic electron affinities (EAs) for the heteroatomic molecule SO 4. The method involves the approximation to the complete basis set (CBS) limit at the MP2 level. The zero-point vibrational energy correction, the diagonal Born-Oppenheimer correction, and the scalar relativistic effect correction have been also made in the calculations. The present MP2/CBS predictions are found to be in good accord with the available experimental values.

  16. Survey of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and non-ortho-polychlorinated biphenyls in US meat and poultry, 2012-13: toxic equivalency levels, patterns, temporal trends and implications.

    Science.gov (United States)

    Lupton, Sara J; O'Keefe, Margaret; Muñiz-Ortiz, Jorge G; Clinch, Nelson; Basu, Pat

    2017-07-11

    The US Department of Agriculture (USDA) conducts a statistically based survey of the domestic meat supply (beef, pork, chicken and turkey) to determine current levels of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and non-ortho-polychlorinated biphenyls (no-PCBs) every 5 years. Fat samples for each slaughter class were collected from US federally licensed slaughter facilities. The samples were processed and analysed for 17 PCDD/Fs and three no-PCBs. The sum of PCDD, PCDF and no-PCB toxic equivalencies (sum-TEQ) calculated using 2005 toxic-equivalency factors for all slaughter classes ranged from non-detect (n.d.) to 6.47 pg TEQ g(-1) lipid. The median sum-TEQs, when n.d. = 0.5 LOD, for beef, pork, chicken and turkey were 0.66, 0.12, 0.13 and 0.34 pg TEQ g(-1) lipid respectively. A comparison of the current survey with the previous three surveys shows a declining trend, with decreasing differences between medians; differences between the median sum-TEQs from 2007-08 and 2012-13 were -10%, -29%, -33% and -25% for beef, pork, chicken and turkey respectively. Several beef samples underwent further characterisation and congener patterns from these beef samples suggested pentachlorophenol treated wood as the likely exposure source. US consumer exposure to these compounds is relatively low and no slaughter class contributed more than 26% to the US Environmental Protection Agency (USEPA) chronic oral reference dose of 0.7 pg TEQ kg(-1) bw day(-1).

  17. Gain and Bandwidth Enhancement of Ferrite-Loaded CBS Antenna Using Material Shaping and Positioning

    Science.gov (United States)

    Askarian Amiri, Mikal

    Loading a cavity-backed slot (CBS) antenna with ferrite material and applying a biasing static magnetic field can be used to control its resonant frequency. Such a mechanism results in a frequency reconfigurable antenna. However, placing a lossy ferrite material inside the cavity can reduce the gain or negatively impact the impedance bandwidth. This thesis develops guidelines, based on a non-uniform applied magnetic field and non-uniform magnetic field internal to the ferrite specimen, for the design of ferrite-loaded CBS antennas which enhance their gain and tunable bandwidth by shaping the ferrite specimen and judiciously locating it within the cavity. To achieve these objectives, it is necessary to examine the influence of the shape and relative location of the ferrite material, and also the proximity of the ferrite specimen from the probe on the DC magnetic field and RF electric field distributions inside the cavity. The geometry of the probe and its impacts on figures-of-merit of the antenna is of interest as well. Two common cavity backed-slot antennas (rectangular and circular cross-section) were designed, and corresponding simulations and measurements were performed and compared. The cavities were mounted on 30 cm × 30 cm perfect electric conductor (PEC) ground planes and partially loaded with ferrite material. The ferrites were biased with an external magnetic field produced by either an electromagnet or permanent magnets. Simulations were performed using FEM-based commercial software, Ansys' Maxwell 3D and HFSS. Maxwell 3D is utilized to model the non-uniform DC applied magnetic field and non-uniform magnetic field internal to the ferrite specimen; HFSS however, is used to simulate and obtain the RF characteristics of the antenna. To validate the simulations they were compared with measurements performed in ASU's EM Anechoic Chamber. After many examinations using simulations and measurements, some optimal designs guidelines with respect to the gain

  18. Kommentar: Nye kontekster for områdestudier på CBS

    DEFF Research Database (Denmark)

    Helmersen, Ole; Ashbee, Edward

    2013-01-01

    Center for Amerikanske Studier er lukket ned, men dette bør ikke være enden for områdestudier på CBS.......Center for Amerikanske Studier er lukket ned, men dette bør ikke være enden for områdestudier på CBS....

  19. Novel structural arrangement of nematode cystathionine β-synthases: characterization of Caenorhabditis elegans CBS-1.

    Science.gov (United States)

    Vozdek, Roman; Hnízda, Aleš; Krijt, Jakub; Kostrouchová, Marta; Kožich, Viktor

    2012-04-15

    CBSs (cystathionine β-synthases) are eukaryotic PLP (pyridoxal 5 *-phosphate)-dependent proteins that maintain cellular homocysteine homoeostasis and produce cystathionine and hydrogen sulfide. In the present study, we describe a novel structural arrangement of the CBS enzyme encoded by the cbs-1 gene of the nematode Caenorhabditis elegans. The CBS-1 protein contains a unique tandem repeat of two evolutionarily conserved catalytic regions in a single polypeptide chain. These repeats include a catalytically active C-terminal module containing a PLP-binding site and a less conserved N-terminal module that is unable to bind the PLP cofactor and cannot catalyse CBS reactions, as demonstrated by analysis of truncated variants and active-site mutant proteins. In contrast with other metazoan enzymes, CBS-1 lacks the haem and regulatory Bateman domain essential for activation by AdoMet (S-adenosylmethionine) and only forms monomers. We determined the tissue and subcellular distribution of CBS-1 and showed that cbs-1 knockdown by RNA interference leads to delayed development and to an approximately 10-fold elevation of homocysteine concentrations in nematode extracts. The present study provides the first insight into the metabolism of sulfur amino acids and hydrogen sulfide in C. elegans and shows that nematode CBSs possess a structural feature that is unique among CBS proteins.

  20. The CBS domain protein MJ0729 of Methanocaldococcus jannaschii binds DNA.

    Science.gov (United States)

    Aguado-Llera, David; Oyenarte, Iker; Martínez-Cruz, Luis Alfonso; Neira, José L

    2010-11-05

    The cystathionine beta-synthase (CBS) domains function as regulatory motifs in several proteins. Elucidating how CBS domains exactly work is relevant because several genetic human diseases have been associated with mutations in those motifs. Here, we show, for the first time, that a CBS domain binds calf-thymus DNA and E-boxes recognized by transcription factors. We have carried out the DNA-binding characterization of the CBS domain protein MJ0729 from Methanocaldococcus jannaschii by biochemical and spectroscopic techniques. Binding induces conformational changes in the protein, and involves the sole tryptophan residue. The apparent dissociation constant for the E-boxes is ∼10 μM. These results suggest that CBS domains might interact with DNA. Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  1. Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

    Science.gov (United States)

    Li, Rongpeng; Jiang, Yuji; Wang, Xinfeng; Yang, Jingjing; Gao, Yuan; Zi, Xiaoli; Zhang, Xia; Gao, Haofeng; Hu, Nan

    2013-01-01

    A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

  2. Composition and content of CBs, CDFs and CNs of Clophen A60

    Energy Technology Data Exchange (ETDEWEB)

    Taniyasu, S.; Horii, Y.; Hanari, N.; Yamashita, N. [National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba (Japan); Falandysz, J.; Swietojanska, A.; Flisak, M. [Gdansk Univ. (Poland). Dept. of Environmental Chemistry and Ecotoxicology; Petrick, G. [Kiel Univ. (Germany). Inst. for Marine Research

    2004-09-15

    Introduction Chlorobiphenyls (CBs; polychlorinated biphenyls, PCBs) starting from the year 1929 periodically become increasingly important and highly welcomed industrial compounds but further claimed as globally diffused environmental, food and human body contaminants. Those compounds, when contained in any man-derived product at concentration exceeding 50 mg/kg nowadays are considered as hazardous waste which should be safely disposed world-wide. Chlorobiphenyls form very complex mixtures with tens of congeners which are found and quantified as principal constituents in an original technical CB formulations manufactured. The technical CB mixtures such as Aroclor, Kanechlor or Delor series usually contain by-side trace constituents and including dioxin-like highly toxic substances such as chlorodibenzofurans (CDFs; polychlorinated dibenzofurans, PCDFs) and chloronaphthalenes (CNs; polychlorinated naphthalenes, PCNs), while no chlorodibenzo-p-dioxins (CDDs; polychlorinated dibenzo-pdioxins, PCDDs) could be detected. An introduced concept ''to take a short cut'' in analysis and assessment of the total CBs content of the sample includes measurement of selected ''indicator'' congeners, i.e. IUPAC Nos. 28, 52, 138, 153 and 180. The state-of-art approach when related to CBs environmental issues is to know and understand composition, absolute concentrations, environmental and body distribution, and elimination rates of all congeners constituting or contaminating the sample. Clophen A60 is one of the several technical chlorobiphenyl formulations of the Clophen family manufactured in the past by the Bayer AG (former I.G. Farben) in Germany. Congenerspecific data on CB composition of Clophen A60 as well as of Clophen A30, A40, A50, and T64 have been a subject of several earlier investigations. In this report qualitative and quantitative data are presented on chlorobiphenyl constituents of technical Clophen A60 and on it's some

  3. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE).

    Science.gov (United States)

    Asimakopoulou, Antonia; Panopoulos, Panagiotis; Chasapis, Christos T; Coletta, Ciro; Zhou, Zongmin; Cirino, Giuseppe; Giannis, Athanassios; Szabo, Csaba; Spyroulias, Georgios A; Papapetropoulos, Andreas

    2013-06-01

    Hydrogen sulfide (H₂S) is a signalling molecule that belongs to the gasotransmitter family. Two major sources for endogenous enzymatic production of H₂S are cystathionine β synthase (CBS) and cystathionine γ lyase (CSE). In the present study, we examined the selectivity of commonly used pharmacological inhibitors of H₂S biosynthesis towards CSE and CBS. To address this question, human CSE or CBS enzymes were expressed and purified from Escherichia coli as fusion proteins with GSH-S-transferase. After purification, the activity of the recombinant enzymes was tested using the methylene blue method. β-Cyanoalanine (BCA) was more potent in inhibiting CSE than propargylglycine (PAG) (IC₅₀ 14 ± 0.2 μM vs. 40 ± 8 μM respectively). Similar to PAG, L-aminoethoxyvinylglycine (AVG) only inhibited CSE, but did so at much lower concentrations. On the other hand, aminooxyacetic acid (AOAA), a frequently used CBS inhibitor, was more potent in inhibiting CSE compared with BCA and PAG (IC₅₀ 1.1 ± 0.1 μM); the IC₅₀ for AOAA for inhibiting CBS was 8.5 ± 0.7 μM. In line with our biochemical observations, relaxation to L-cysteine was blocked by AOAA in aortic rings that lacked CBS expression. Trifluoroalanine and hydroxylamine, two compounds that have also been used to block H₂S biosynthesis, blocked the activity of CBS and CSE. Trifluoroalanine had a fourfold lower IC₅₀ for CBS versus CSE, while hydroxylamine was 60-fold more selective against CSE. In conclusion, although PAG, AVG and BCA exhibit selectivity in inhibiting CSE versus CBS, no selective pharmacological CBS inhibitor is currently available. © 2013 The Authors. British Journal of Pharmacology © 2013 The British Pharmacological Society.

  4. Betaine supplementation is less effective than methionine restriction in correcting phenotypes of CBS deficient mice.

    Science.gov (United States)

    Gupta, Sapna; Wang, Liqun; Kruger, Warren D

    2016-01-01

    Cystathionine beta synthase (CBS) deficiency is a recessive inborn error of metabolism characterized by elevated serum total homocysteine (tHcy). Betaine supplementation, which can lower tHcy by stimulating homocysteine remethylation to methionine, is often given to CBS deficient patients in combination with other treatments such as methionine restriction and supplemental B-vitamins. However, the effectiveness of betaine supplementation by itself in the treatment of CBS deficiency has not been well explored. Here, we have examined the effect of a betaine supplemented diet on the Tg-I278T Cbs (-/-) mouse model of CBS deficiency and compared its effectiveness to our previously published data using a methionine restricted diet. Tg-I278T Cbs (-/-) mice on betaine, from the time of weaning until for 240 days of age, had a 40 % decrease in mean tHcy level and a 137 % increase in serum methionine levels. Betaine-treated Tg-I278T Cbs (-/-) mice also exhibited increased levels of betaine-dependent homocysteine methyl transferase (BHMT), increased levels of the lipogenic enzyme stearoyl-coenzyme A desaturase (SCD-1), and increased lipid droplet accumulation in the liver. Betaine supplementation largely reversed the hair loss phenotype in Tg-I278T Cbs (-/-) animals, but was far less effective than methionine restriction in reversing the weight-loss, fat-loss, and osteoporosis phenotypes. Surprisingly, betaine supplementation had several negative effects in control Tg-I278T Cbs (+/-) mice including decreased weight gain, lean mass, and bone mineral density. Our findings indicate that while betaine supplementation does have some beneficial effects, it is not as effective as methionine restriction for reversing the phenotypes associated with severe CBS deficiency in mice.

  5. Polymorphisms in genes RFC-1/CBS as maternal risk factors for Down syndrome in China.

    Science.gov (United States)

    Wang, Shao-shuai; Wang, Chao; Qiao, Fu-yuan; Lv, Juan-juan; Feng, Ling

    2013-08-01

    To explore the relationship between genetic polymorphisms in reduced folate carrier 1 (RFC-1), cystathionine b-synthase (CBS), two key genes in folate metabolism, and the risk of Down syndrome in China. Genomic DNA was isolated from the peripheral lymphocytes of 104 mothers born children with Down syndrome and 184 age-matched control mothers. Polymerase chain reaction and restriction-fragment length polymorphism were used to examine the polymorphisms of RFC-1 A80G, CBS T833C and the relationship between these genotypes and the risk of Down syndrome was analyzed. We found that there were significant differences between RFC-1 G80G, CBS C833C polymorphisms among mothers of children with Down syndrome than among control mothers, with odds ratio of 1.51 (95 % CI 1.05-2.18), 1.53 (95 % CI 1.07-2.18) respectively. The combined presence of RFC1 mutant alleles and the CBS homozygous mutant allele (15/104) was associated with a 4.81-fold increased risk of having a child with Down syndrome (95 % CI 1.82-12.68, P = 0.0007). We concluded that RFC-1 and CBS gene mutation alleles are related to Down syndrome, and women with mutation RFC-1 G80G, CBS C833C OR combined with RFC-1 A80G and CBS 833TT genotype increase the risk of Down syndrome in China.

  6. The Coaching Behavior Scale for Sport (CBS-S): A psychometric evaluation of the Swedish version.

    Science.gov (United States)

    Carlsson, A; Lundqvist, C

    2016-01-01

    The present study validated a Swedish version of the 47-item Coaching Behavior Scale for Sport (CBS-S). Sample 1 consisted of 506 team sport athletes [262 men and 244 women; mean age: 22.20, standard deviation (SD) = 3.90] distributed across 41 coaches at the two highest national levels of various sports. Athletes completed the CBS-S and established questionnaires of coaching behaviors (LSS), self-confidence (CSAI-2R), and coach-athlete relationship (CART-Q). An additional sample of 39 basketball players (21 men and 18 women; mean age = 17.40, SD = 2.39) completed the CBS-S twice, approximately 4 weeks apart. Confirmatory factor analysis showed an acceptable model fit for the seven-factor version of the CBS-S, although two items of the negative personal rapport subscale displayed insufficient factor loadings. Correlations between the subscales of the CBS-S and established instruments were in accordance with theoretical expectations, supporting the concurrent validity. Cronbach's alpha (> 0.82) for all dimensions provided support for the reliability of the CBS-S, and test-retest correlations indicated moderate stability over time. Cultural differences in the assessment of coaching behaviors and the usability of the CBS-S by coaches for self-reflection and development are discussed. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. A CBS domain-containing pyrophosphatase of Moorella thermoacetica is regulated by adenine nucleotides

    Science.gov (United States)

    Jämsen, Joonas; Tuominen, Heidi; Salminen, Anu; Belogurov, Georgiy A.; Magretova, Natalia N.; Baykov, Alexander A.; Lahti, Reijo

    2007-01-01

    CBS (cystathionine β-synthase) domains are found in proteins from all kingdoms of life, and point mutations in these domains are responsible for a variety of hereditary diseases in humans; however, the functions of CBS domains are not well understood. In the present study, we cloned, expressed in Escherichia coli, and characterized a family II PPase (inorganic pyrophosphatase) from Moorella thermoacetica (mtCBS-PPase) that has a pair of tandem 60-amino-acid CBS domains within its N-terminal domain. Because mtCBS-PPase is a dimer and requires transition metal ions (Co2+ or Mn2+) for activity, it resembles common family II PPases, which lack CBS domains. The mtCBS-PPase, however, has lower activity than common family II PPases, is potently inhibited by ADP and AMP, and is activated up to 1.6-fold by ATP. Inhibition by AMP is competitive, whereas inhibition by ADP and activation by ATP are both of mixed types. The nucleotides are effective at nanomolar (ADP) or micromolar concentrations (AMP and ATP) and appear to compete for the same site on the enzyme. The nucleotide-binding affinities are thus 100–10000-fold higher than for other CBS-domain-containing proteins. Interestingly, genes encoding CBS-PPase occur most frequently in bacteria that have a membrane-bound H+-translocating PPase with a comparable PPi-hydrolysing activity. Our results suggest that soluble nucleotide-regulated PPases act as amplifiers of metabolism in bacteria by enhancing or suppressing ATP production and biosynthetic reactions at high and low [ATP]/([AMP]+[ADP]) ratios respectively. PMID:17714078

  8. Human cystathionine β-synthase (CBS) contains two classes of binding sites for S-adenosylmethionine (SAM): complex regulation of CBS activity and stability by SAM.

    Science.gov (United States)

    Pey, Angel L; Majtan, Tomas; Sanchez-Ruiz, Jose M; Kraus, Jan P

    2013-01-01

    CBS (cystathionine β-synthase) is a multidomain tetrameric enzyme essential in the regulation of homocysteine metabolism, whose activity is enhanced by the allosteric regulator SAM (S-adenosylmethionine). Missense mutations in CBS are the major cause of inherited HCU (homocystinuria). In the present study we apply a novel approach based on a combination of calorimetric methods, functional assays and kinetic modelling to provide structural and energetic insight into the effects of SAM on the stability and activity of WT (wild-type) CBS and seven HCU-causing mutants. We found two sets of SAM-binding sites in the C-terminal regulatory domain with different structural and energetic features: a high affinity set of two sites, probably involved in kinetic stabilization of the regulatory domain, and a low affinity set of four sites, which are involved in the enzyme activation. We show that the regulatory domain displays a low kinetic stability in WT CBS, which is further decreased in many HCU-causing mutants. We propose that the SAM-induced stabilization may play a key role in modulating steady-state levels of WT and mutant CBS in vivo. Our strategy may be valuable for understanding ligand effects on proteins with a complex architecture and their role in human genetic diseases and for the development of novel pharmacological strategies.

  9. A tracer test at the Beowawe geothermal field, Nevada, using fluorescein and tinopal CBS

    Energy Technology Data Exchange (ETDEWEB)

    Rose, P.E.; Adams, M.C. [Univ. of Utah, Salt Lake City, UT (United States); Benoit, D. [Oxbow Power Services, Inc., Reno, NV (United States)

    1995-12-31

    An interwell tracer test using fluorescein and tinopal CBS was performed at the Beowawe geothermal field in north-central Nevada in order to assess the effects of recent changes to the injection strategy. Fluorescein return curves established injection-production flow patterns and verified that produced water is being reinjected into a region of the reservoir that is in excellent communication with the production wells. An analysis of the tinopal CBS return curves indicated that tinopal CBS was apparently strongly adsorbed onto the reservoir rock. The fluorescein return curves were used to estimate the overall (fractures and matrix) reservoir volume.

  10. Quantitative description of the growth of Saccharomyces cerevisiae CBS 426 on a mixed substrate of glucose and ethanol

    Energy Technology Data Exchange (ETDEWEB)

    Geurts, T.G.E.; De Kok, H.E.; Roels, J.A.

    1980-01-01

    S. cerevisiae CBS 426 was grown aerobically in continuous culture with a mixture of glucose and EtOH as the C source. The flows of biomass, glucose, EtOH, O, and CO/sub 2/ were measured. A model for growth with 2 substrates was derived. Application of this model to the above-mentioned system yielded values for YATP and P/O. The joint confidence regions for these parameters were calculated. The relevance to industrial production of bakers' yeast is discussed.

  11. Cbs (Contrastrain Based Schedulling Adalah Faktor Penentu Keberhasilan Perusahanan Printing

    Directory of Open Access Journals (Sweden)

    Hendra Achmadi

    2010-06-01

    Full Text Available In a highly competitive industry faces today ranging from small or home-based printing to using machine that can print offset a hundred thousand copies per hour. But, the increasing competition resulted in requiring a faster production time from order entry, print proff until the production process to delivery to customers. Often times in case of orders which will result in the concurrent PPIC will experience vertigo in the setting of production schedules which have concurrent delivery time. Often will end up with no receipt of orders due to difficulties in the production schedule, especially if the orders require the same offset machine and cylinder wear the same length, while the number of cylinders is limited. Therefore, the printing company should be able to do so in the conduct of a penetration timing of production can easily be simulated and implemented on the ground. CBS (Base Constraint scheduling is a technique to do the scheduling of production so that production can be carried out smoothly and quickly that fulfill the promise made to customers. In scheduling, there are several techniques that can be used are: FCFS (First Came First Serve, EDD (Earliest Date, and LCLS (Last Came Last Serve. So, it is required to be able to do way better scheduling to get results quickly in this fast changing schedules.

  12. CBS (CONTRASTRAIN BASED SCHEDULLING ADALAH FAKTOR PENENTU KEBERHASILAN PERUSAHANAN PRINTING

    Directory of Open Access Journals (Sweden)

    Hendra Achmadi

    2010-05-01

    Full Text Available In a highly competitive industry faces today ranging from small or home-based printing to using machine that can print offset a hundred thousand copies per hour. But, the increasing competition resulted in requiring a faster production time from order entry, print proff until the production process to delivery to customers. Often times in case of orders which will result in the concurrent PPIC will experience vertigo in the setting of production schedules which have concurrent delivery time. Often will end up with no receipt of orders due to difficulties in the production schedule, especially if the orders require the same offset machine and cylinder wear the same length, while the number of cylinders is limited. Therefore, the printing company should be able to do so in the conduct of a penetration timing of production can easily be simulated and implemented on the ground. CBS (Base Constraint scheduling is a technique to do the scheduling of production so that production can be carried out smoothly and quickly that fulfill the promise made to customers. In scheduling, there are several techniques that can be used are: FCFS (First Came First Serve, EDD (Earliest Date, and LCLS (Last Came Last Serve. So, it is required to be able to do way better scheduling to get results quickly in this fast changing schedules.

  13. Reduced response of Cystathionine Beta-Synthase (CBS) to S-Adenosylmethionine (SAM): Identification and functional analysis of CBS gene mutations in Homocystinuria patients.

    Science.gov (United States)

    Mendes, Marisa I S; Colaço, Henrique G; Smith, Desirée E C; Ramos, Rúben J J F; Pop, Ana; van Dooren, Silvy J M; Tavares de Almeida, Isabel; Kluijtmans, Leo A J; Janssen, Mirian C H; Rivera, Isabel; Salomons, Gajja S; Leandro, Paula; Blom, Henk J

    2014-03-01

    A reduced response of cystathionine beta-synthase (CBS) to its allosteric activator S-adenosylmethionine (SAM) has been reported to be a cause of CBS dysfunction in homocystinuria patients. In this work we performed a retrospective analysis of fibroblast data from 62 homocystinuria patients and found that 13 of them presented a disturbed SAM activation. Their genotypic background was identified and the corresponding CBS mutant proteins were produced in E. coli. Nine distinct mutations were detected in 22 independent alleles: the novel mutations p.K269del, p.P427L, p.S500L and p.L540Q; and the previously described mutations p.P49L, p.C165Rfs*2, p.I278T, p.R336H and p.D444N. Expression levels and residual enzyme activities, determined in the soluble fraction of E. coli lysates, strongly correlated with the localization of the affected amino acid residue. C-terminal mutations lead to activities in the range of the wild-type CBS and to oligomeric forms migrating faster than tetramers, suggesting an abnormal conformation that might be responsible for the lack of SAM activation. Mutations in the catalytic core were associated with low protein expression levels, decreased enzyme activities and a higher content of high molecular mass forms. Furthermore, the absence of SAM activation found in the patients' fibroblasts was confirmed for all but one of the characterized recombinant proteins (p.P49L). Our study experimentally supports a deficient regulation of CBS by SAM as a frequently found mechanism in CBS deficiency, which should be considered not only as a valuable diagnostic tool but also as a potential target for the development of new therapeutic approaches in classical homocystinuria.

  14. Analysis of MTHFR, CBS, Glutathione, Taurine, and Hydrogen Sulfide Levels in Retinas of Hyperhomocysteinemic Mice.

    Science.gov (United States)

    Cui, Xuezhi; Navneet, Soumya; Wang, Jing; Roon, Penny; Chen, Wei; Xian, Ming; Smith, Sylvia B

    2017-04-01

    Hyperhomocysteinemia (Hhcy) is implicated in certain retinal neurovascular diseases, although whether it is causative remains uncertain. In isolated ganglion cells (GCs), mild Hhcy induces profound death, whereas retinal phenotypes in Hhcy mice caused by mutations in remethylation (methylene tetrahydrofolatereductase [Mthfr+/-]) or transsulfuration pathways (cystathionine β-synthase [Cbs+/-]) demonstrate mild GC loss and mild vasculopathy. The current work investigated compensation in vivo of one pathway for the other, and, because the transsulfuration pathway yields cysteine necessary for formation of glutathione (GSH), taurine, and hydrogen sulfide (H2S), they were analyzed also. Retinas isolated from wild-type (WT), Mthfr+/-, and Cbs+/- mice (12 and 22 weeks) were analyzed for methylene tetrahydrofolate reductase (MTHFR), cystathionine-β-synthase (CBS), and cystathionase (CTH) RNA/protein levels. Retinas were evaluated for levels of reduced:oxidized GSH (GSH:GSSG), Slc7a11 (xCT), taurine, taurine transporter (TAUT), and H2S. Aside from decreased CBS RNA/protein levels in Cbs+/- retinas, there were minimal alterations in remethylation/transsulfuration pathways in the two mutant mice strains. Glutathione and taurine levels in Mthfr+/- and Cbs+/- retinas were similar to WT, which may be due to robust levels of xCT and TAUT in mutant retinas. Interestingly, levels of H2S were markedly increased in retinas of Mthfr+/- and Cbs+/- mice compared with WT. Ganglion cell loss and vasculopathy observed in Mthfr+/- and Cbs+/- mouse retinas may be milder than expected, not because of compensatory increases of enzymes in remethylation/transsulfuration pathways, but because downstream transsulfuration pathway products GSH, taurine, and H2S are maintained at robust levels. Elevation of H2S is particularly intriguing owing to neuroprotective properties reported for this gasotransmitter.

  15. "To år med CBS Maritime - her er de første resultater"

    DEFF Research Database (Denmark)

    Sornn-Friese, Henrik; Hansen, Carsten Ørts

    2014-01-01

    Rederier: CBS Maritime har netop offentliggjort en statusrapport på udviklingen siden starten i 2012 og med tal for udviklingen på de nye maritime uddannelser. Forfatterne fremlægger nogle af resultaterne her.......Rederier: CBS Maritime har netop offentliggjort en statusrapport på udviklingen siden starten i 2012 og med tal for udviklingen på de nye maritime uddannelser. Forfatterne fremlægger nogle af resultaterne her....

  16. Cystathionine beta-synthase (CBS) contributes to advanced ovarian cancer progression and drug resistance.

    Science.gov (United States)

    Bhattacharyya, Sanjib; Saha, Sounik; Giri, Karuna; Lanza, Ian R; Nair, K Sreekumar; Jennings, Nicholas B; Rodriguez-Aguayo, Cristian; Lopez-Berestein, Gabriel; Basal, Eati; Weaver, Amy L; Visscher, Daniel W; Cliby, William; Sood, Anil K; Bhattacharya, Resham; Mukherjee, Priyabrata

    2013-01-01

    Epithelial ovarian cancer is the leading cause of gynecologic cancer deaths. Most patients respond initially to platinum-based chemotherapy after surgical debulking, however relapse is very common and ultimately platinum resistance emerges. Understanding the mechanism of tumor growth, metastasis and drug resistant relapse will profoundly impact the therapeutic management of ovarian cancer. Using patient tissue microarray (TMA), in vitro and in vivo studies we report a role of of cystathionine-beta-synthase (CBS), a sulfur metabolism enzyme in ovarian carcinoma. We report here that the expression of cystathionine-beta-synthase (CBS), a sulfur metabolism enzyme, is common in primary serous ovarian carcinoma. The in vitro effects of CBS silencing can be reversed by exogenous supplementation with the GSH and H2S producing chemical Na2S. Silencing CBS in a cisplatin resistant orthotopic model in vivo by nanoliposomal delivery of CBS siRNA inhibits tumor growth, reduces nodule formation and sensitizes ovarian cancer cells to cisplatin. The effects were further corroborated by immunohistochemistry that demonstrates a reduction of H&E, Ki-67 and CD31 positive cells in si-RNA treated as compared to scrambled-RNA treated animals. Furthermore, CBS also regulates bioenergetics of ovarian cancer cells by regulating mitochondrial ROS production, oxygen consumption and ATP generation. This study reports an important role of CBS in promoting ovarian tumor growth and maintaining drug resistant phenotype by controlling cellular redox behavior and regulating mitochondrial bioenergetics. The present investigation highlights CBS as a potential therapeutic target in relapsed and platinum resistant ovarian cancer.

  17. Cystathionine beta-synthase (CBS contributes to advanced ovarian cancer progression and drug resistance.

    Directory of Open Access Journals (Sweden)

    Sanjib Bhattacharyya

    Full Text Available BACKGROUND: Epithelial ovarian cancer is the leading cause of gynecologic cancer deaths. Most patients respond initially to platinum-based chemotherapy after surgical debulking, however relapse is very common and ultimately platinum resistance emerges. Understanding the mechanism of tumor growth, metastasis and drug resistant relapse will profoundly impact the therapeutic management of ovarian cancer. METHODS/PRINCIPAL FINDINGS: Using patient tissue microarray (TMA, in vitro and in vivo studies we report a role of of cystathionine-beta-synthase (CBS, a sulfur metabolism enzyme in ovarian carcinoma. We report here that the expression of cystathionine-beta-synthase (CBS, a sulfur metabolism enzyme, is common in primary serous ovarian carcinoma. The in vitro effects of CBS silencing can be reversed by exogenous supplementation with the GSH and H2S producing chemical Na2S. Silencing CBS in a cisplatin resistant orthotopic model in vivo by nanoliposomal delivery of CBS siRNA inhibits tumor growth, reduces nodule formation and sensitizes ovarian cancer cells to cisplatin. The effects were further corroborated by immunohistochemistry that demonstrates a reduction of H&E, Ki-67 and CD31 positive cells in si-RNA treated as compared to scrambled-RNA treated animals. Furthermore, CBS also regulates bioenergetics of ovarian cancer cells by regulating mitochondrial ROS production, oxygen consumption and ATP generation. This study reports an important role of CBS in promoting ovarian tumor growth and maintaining drug resistant phenotype by controlling cellular redox behavior and regulating mitochondrial bioenergetics. CONCLUSION: The present investigation highlights CBS as a potential therapeutic target in relapsed and platinum resistant ovarian cancer.

  18. Enhanced anti-topoisomerase II activity by mucoadhesive 4-CBS-chitosan/poly (lactic acid) nanoparticles.

    Science.gov (United States)

    Songsurang, Kultida; Suvannasara, Phruetchika; Phurat, Chuttree; Puthong, Songchan; Siraleartmukul, Krisana; Muangsin, Nongnuj

    2013-11-06

    In this study, the biodegradable mucoadhesive 4-carboxybenzensulfonamide chitosan (4-CBS-chitosan)/poly (lactic acid) (PLA) nanoparticles were fabricated by the electrospray ionization technique for enhancing anti-topoisomerase II (Topo II) activity. The obtained (4-CBS-chitosan/PLA)-DOX nanoparticles were characterized using SEM, particle size analyzer. We emphasis on encapsulation efficiency, in vitro drug release behavior and also performed in vitro studies of Topo II inhibitory activity using gel electrophoresis. In addition, the cytotoxicity of the 4-CBS-chitosan/PLA nanoparticles using MTT assay was also studied. The mean particle size of spherical shaped (4-CBS-chitosan/PLA)-DOX is less than 300 nm. The DOX loaded 4-CBS-chitosan/PLA composite nanoparticles produced high entrapment efficiency of 85.8% and provided the prolonged release of DOX extended to 26 days and also still had strong Topo II inhibitory activity up to 77.4%. Overall, it was shown that 4-CBS-chitosan/PLA nanoparticles could be promising carriers for controlled delivery of anticancer drugs. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. The CBS/CSE system: a potential therapeutic target in NAFLD?

    Science.gov (United States)

    Sarna, Lindsei K; Siow, Yaw L; O, Karmin

    2015-01-01

    Non-alcoholic fatty liver disease (NAFLD) is a broad spectrum liver disorder diagnosed in patients without a history of alcohol abuse. NAFLD is growing at alarming rates worldwide. Its pathogenesis is complex and incompletely understood. The cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) system regulates homocysteine and cysteine metabolism and contributes to endogenous hydrogen sulfide (H2S) biosynthesis. This review summarizes our current understanding of the hepatic CBS/CSE system, and for the first time, positions this system as a potential therapeutic target in NAFLD. As will be discussed, the CBS/CSE system is highly expressed and active in the liver. Its dysregulation, presenting as alterations in circulating homocysteine and (or) H2S levels, has been reported in NAFLD patients and in NAFLD-associated co-morbidities such as obesity and type 2 diabetes. Intricate links between the CBS/CSE system and a number of metabolic and stress related molecular mediators have also emerged. Various dysfunctions in the hepatic CBS/CSE system have been reported in animal models representative of each NAFLD spectrum. It is anticipated that a newfound appreciation for the hepatic CBS/CSE system will emerge that will improve our understanding of NAFLD pathogenesis, and give rise to new prospective targets for management of this disorder.

  20. Novel Compound Heterozygous CBS Mutations Cause Homocystinuria in a Han Chinese Family.

    Science.gov (United States)

    Gong, Bo; Liu, Liping; Li, Zhiwei; Ye, Zimeng; Xiao, Ying; Zeng, Guangqun; Shi, Yi; Wang, Yumeng; Feng, Xiaoyun; Li, Xiulan; Hao, Fang; Liu, Xiaoqi; Qu, Chao; Li, Yuanfeng; Mu, Guoying; Yang, Zhenglin

    2015-12-15

    The cystathionine β-synthase (CBS) gene has been shown to be related to homocystinuria. This study was aimed to detect the mutations in CBS in a Han Chinese family with homocystinuria. A four-generation family from Shandong Province of China was recruited in this study. All available members of the family underwent comprehensive medical examinations. Genomic DNA was collected from peripheral blood of all the participants. The coding sequence of CBS was amplified by polymerase chain reaction (PCR), followed by direct DNA sequencing. Among all the family members, three affected individuals showed typical clinical features of homocystinuria. Two novel compound heterozygous mutations in the CBS gene, c.407T > C (p. L136P) and c.473C > T (p.A158V), were identified by sequencing analysis in this family. Both of the two missense mutations were detected in the three patients. Other available normal individuals, including the patients' parents, grand parents, her younger sister and brother in this family either carried one of the two mutations, or none. In addition, the two mutations were not found in 600 ethnically matched normal controls. This study provides a mutation spectrum of CBS resulting in homocystinuriain a Chinese population, which may shed light on the molecular pathogenesis and clinical diagnosis of CBS-associated homocystinuria.

  1. The analyse of chlorobenzenes (CBS in the soil environment

    Directory of Open Access Journals (Sweden)

    Ferdi Brahushi

    2013-05-01

    Full Text Available Chlorobenzenes (CBs are a group of substituted benzene compounds with only chlorine and hydrogen atoms on the benzene ring. They are mainly used as solvents, pesticides, fire retardants, degreasers, heat transmitters, chemical intermediates and are released in the environment due to their extensive use. This study presents a developing method for determination of chlorobenzenes in the soil, including extraction techniques, concentration, clean up and injection in GC-ECD system which was equipped with a DB-5 capillary column and an AS 2000 autosampler. The detection limit of the method was calculated based on the chlorobenzene recoveries of the presented analytical method and on the detection limits in GC of each chlorobenzes. The extraction with ASE technique presented the best results as the recoveries of MCB, 1,2,4-TCB and HCB from soil with were 100,5±2,5, 88,0±3,3 and 88,0±5,0, respectively. The detection limits of chlorobenzens in the GC-ECD varied from 0,38 pg/µl for HCB to 250,00 pg/µl for MCB. Also, the total recovery of chlorobenzenes was high for HCB (84,13 ± 4,30 and very low for MCB (9,80 ± 1,16. The values of method detection limits of chlorobenzenes in soil were from 0,14 µg/kg soil for HCB to 765,31 µg/kg soil for MCB, whereas other clorobenzenes had intermediate values. Therefore, by using this determination method, high chlorinated benzenes as HCB and PCB could be detected in very small amounts in soil samples.

  2. Determination of the Component of Fluorescent Whitening Agent CBS-127 by HPLC%HPLC法测定荧光增白剂CBS-127的含量

    Institute of Scientific and Technical Information of China (English)

    李自红; 魏悦; 范毅; 董建军

    2013-01-01

    The method for determinating the content of Fluorescent Whitening Agent CBS-127 by HPLC was established.Sunfire C18 column (4.6 mm×150 mm,5 μm) was used with methanol-water (90%:10%) as the mobile phase.The flow rate of the mobile phase was 1.0 mL/min.The detection wavelength was 356 nm.The column temperature was 40 C.There was good linear relationship in the range of 0.5~2.5 μg (r=0.999 9).The recovery rate was 99.78% and RSD value is 0.7% (n=6).This method is high accuracy,good reproducibility,reliable results,which is an effective method for the Fluorescent Whitening Agent CBS-127.%建立了高效液相色谱法测定荧光增白剂CBS-127含量的方法.采用Sunfire C18 (4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水(90%:10%),流速为1.0 mL/min,紫外检测波长为356 nm,柱温40℃.CBS-127在0.5~2.5 μg范围内有良好线性关系(r=0.999 9);加样回收率为99.78%,RSD值为0.7%(n=6).该方法精密度高、重现性好、结果准确可靠,为荧光增白剂CBS-127的质量评价提供了有效的方法.

  3. CBs levels in deer tissue samples following an accidental release from a special waste treatment center : 2003 results

    Energy Technology Data Exchange (ETDEWEB)

    Mackenzie, A.; Zhang, W.; Gabos, S.; Schopflocher, D.; Robb, J. [Alberta Health and Wellness, Edmonton, AB (Canada). Health Surveillance; Ikonomou, M. [Inst. of Ocean Sciences, Sidney, BC (Canada)

    2005-07-01

    High levels of dioxins and furans (PCDD/Fs) were observed in deer and mouse tissues examined after an accidental release of PCDD/F contaminants in the Swan Hills area of Alberta. This paper provided details of follow-up sampling conducted to assess changes in CB concentrations of whitetail deer and mule deer in the region. Field collection was carried out in December 2002 and February 2003. Representative muscle, liver and fat samples were taken from each deer. Four aliquots from each sample were collected by carbon-fibre fractionation. All possible 209 CB congeners were measured with minimum isomeric interference. Fractions were analyzed using high resolution gas chromatography (HRGC) and high resolution mass spectrometry (HRMS). Concentrations of identified compounds were calculated using mean relative response factors determined from calibration standard runs. Results showed that 55 of 209 CB congeners were not detected in the fat samples. The mean level of CB homologues in the 2003 study was 39 ng/g for the lipid basis in the muscle, 92 ng/g in the liver, and 20.5 ng/g in the fat. Di-ortho CBs constituted 72 per cent to 74 per cent of CBs for the samples. Concentration levels of SCBs were lower than studies conducted in previous years. It was concluded that overall levels of SCBs and SCBs TEQ in the liver and muscle samples in 2003 significantly declined when compared with levels observed in 1997, 1999, and 2001. 6 refs., 4 tabs.

  4. Transcultural validation of the ALS-CBS Cognitive Section for the Brazilian population.

    Science.gov (United States)

    Branco, Lucas M T; Zanao, Tamires; De Rezende, Thiago J; Casseb, Raphael F; Balthazar, Marcio F; Woolley, Susan C; França, Marcondes C

    2017-02-01

    Cognitive decline (CD) is common but often under-recognized in ALS due to the scarcity of adequate cognitive screening methods. In this scenario, the Amyotrophic Lateral Sclerosis Cognitive Behavioural Screen (ALS-CBS) is the most investigated instrument and presents high sensitivity to identify CD. Currently, there are no validated cognitive screening tools for ALS patients in the Brazilian population and little is known about the frequency of ALS related CD in the country. We assessed the accuracy of the Brazilian Portuguese version of ALS-CBS Cognitive Section (ALS-CBS-Br) for classifying the cognitive status of Brazilian patients compared to a standard neuropsychological battery, and estimated the prevalence of CD in the Brazilian ALS population. Among 73 initially recruited ALS patients, 49 were included. Twenty-four patients were excluded due to severe motor disability, FTD diagnosis or non-acceptance. Ten healthy controls were also included. Ten ALS patients (20%) were diagnosed with executive dysfunction (ALSci) based on the battery results. ALS-CBS-Br scores were significantly lower in the ALSci group (p CBS-Br may facilitate the recognition of CD in routine clinical care and complement future studies in our population.

  5. TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity.

    Science.gov (United States)

    Yuan, Bo; Tang, Wei-Hong; Lu, Li-Juan; Zhou, Yuan; Zhu, Hong-Yan; Zhou, You-Lang; Zhang, Hong-Hong; Hu, Chuang-Ying; Xu, Guang-Yin

    2015-07-28

    To investigate the roles of toll-like receptor 4 (TLR4) and nuclear factor (NF)-κB on cystathionine β synthetase (CBS) expression and visceral hypersensitivity in rats. This study used 1-7-wk-old male Sprague-Dawley rats. Western blot analysis was employed to measure the expression of TLR4, NF-κB and the endogenous hydrogen sulfide-producing enzyme CBS in colon dorsal root ganglia (DRG) from control and "irritable bowel syndrome" rats induced by neonatal colonic inflammation (NCI). Colon-specific DRG neurons were labeled with Dil and acutely dissociated to measure excitability with patch-clamp techniques. Immunofluorescence was employed to determine the co-expression of TLR4, NF-κB and CBS in DiI-labeled DRG neurons. NCI significantly upregulated the expression of TLR4 in colon-related DRGs (0.34 ± 0.12 vs 0.72 ± 0.02 for the control and NCI groups, respectively, P colon-specific DRG neurons and reduced the expression of CBS (1.7 ± 0.1 vs 1.1 ± 0.04, P < 0.05) and of the NF-κB subunit p65 (0.8 ± 0.1 vs 0.5 ± 0.1, P < 0.05). Furthermore, the NF-κB-selective inhibitor pyrrolidine dithiocarbamate (PDTC) significantly reduced the upregulation of CBS (1.0 ± 0.1 vs 0.6 ± 0.1, P < 0.05) and attenuated visceral hypersensitivity in the NCI rats. In vitro, incubation of cultured DRG neurons with the TLR4 agonist lipopolysaccharide significantly enhanced the expression of p65 (control vs 8 h: 0.9 ± 0.1 vs 1.3 ± 0.1; control vs 12 h: 0.9 ± 0.1 vs 1.3 ± 0.1, P < 0.05; control vs 24 h: 0.9 ± 0.1 vs 1.6 ± 0.1, P < 0.01) and CBS (control vs 12 h: 1.0 ± 0.1 vs 2.2 ± 0.4; control vs 24 h: 1.0 ± 0.1 vs 2.6 ± 0.1, P < 0.05), whereas the inhibition of p65 via pre-incubation with PDTC significantly reversed the upregulation of CBS expression (1.2 ± 0.1 vs 0.6 ± 0.0, P < 0.01). Our results suggest that the activation of TLR4 by NCI upregulates CBS expression, which is mediated by the NF-κB signaling pathway, thus contributing to visceral hypersensitivity.

  6. Polymorphisms in MTHFR, MS and CBS genes and premature acute myocardial infarction in a Pakistani population.

    Science.gov (United States)

    Iqbal, Mohammad Perwaiz; Iqbal, Khalida; Tareen, Asal Khan; Parveen, Siddiqa; Mehboobali, Naseema; Haider, Ghulam; Iqbal, Saleem Perwaiz

    2016-11-01

    High prevalence of premature coronary heart disease in Pakistanis compared to other populations points towards the genetic predisposition of this population to develop this disease. Since no investigations have been carried out in Pakistan to study the relationship of polymorphisms in genes involved in homocysteine cycle, the objective of the present study was to find out if there is any association of methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C; methionine synthase (MS) A2756G; cystathionine-β-synthase (CBS) 844ins68, G919A polymorphisms with premature acute myocardial infarction (AMI) in a population of Pakistani patients with this disease. In a cross-sectional study, DNA samples of 143 AMI patients (age CBS844ins68 polymorphisms and premature AMI in this population. This indicates that common polymorphisms in MTHFR, MS and CBS genes have no role in premature AMI in Pakistani population.

  7. CBS 128 - A new magnetic degenerate with a 65 megagauss surface field

    Energy Technology Data Exchange (ETDEWEB)

    Saffer, R.A.; Liebert, J.; Wagner, R.M.; Sion, E.M.; Starrfield, S.G. (Steward Observatory, Tucson, AZ (USA); Ohio State Univ., Columbus (USA); Villanova Univ., PA (USA); Arizona State Univ., Tempe (USA))

    1989-08-01

    A new magnetic degenerate, CBS 128, has been discovered through follow-up spectroscopy of the Case Blue Star candidates (Wagner et al., 1988). The spectrum reveals Zeeman-shifted Balmer-line components in a 16,000 K DA atmosphere. The spectroscopic observations have been modeled with a synthetic, magnetically distorted spectrum of a star with a centered dipole field geometry (cf. Latter et al., 1987). The model-derived polar field strength for CBS 128 is 65 megagauss (MG), placing this star's field value between the 30 MG (or less) values of the majority of magnetics and the strong-field cases at or above 100 MG. CBS 128, like the vast majority of the magnetic white dwarfs, has small space motions with respect to the sun, a possible indication of youth and evolution from fairly massive progenitors. 21 refs.

  8. CBS 128 - A new magnetic degenerate with a 65 megagauss surface field

    Science.gov (United States)

    Saffer, Rex A.; Liebert, James; Wagner, R. Mark; Sion, Edward M.; Starrfield, Sumner G.

    1989-08-01

    A new magnetic degenerate, CBS 128, has been discovered through follow-up spectroscopy of the Case Blue Star candidates (Wagner et al., 1988). The spectrum reveals Zeeman-shifted Balmer-line components in a 16,000 K DA atmosphere. The spectroscopic observations have been modeled with a synthetic, magnetically distorted spectrum of a star with a centered dipole field geometry (cf. Latter et al., 1987). The model-derived polar field strength for CBS 128 is 65 megagauss (MG), placing this star's field value between the 30 MG (or less) values of the majority of magnetics and the strong-field cases at or above 100 MG. CBS 128, like the vast majority of the magnetic white dwarfs, has small space motions with respect to the sun, a possible indication of youth and evolution from fairly massive progenitors.

  9. Chaperone therapy for homocystinuria: the rescue of CBS mutations by heme arginate.

    Science.gov (United States)

    Melenovská, Petra; Kopecká, Jana; Krijt, Jakub; Hnízda, Aleš; Raková, Kateřina; Janošík, Miroslav; Wilcken, Bridget; Kožich, Viktor

    2015-03-01

    Classical homocystinuria is caused by mutations in the cystathionine β-synthase (CBS) gene. Previous experiments in bacterial and yeast cells showed that many mutant CBS enzymes misfold and that chemical chaperones enable proper folding of a number of mutations. In the present study, we tested the extent of misfolding of 27 CBS mutations previously tested in E. coli under the more folding-permissive conditions of mammalian CHO-K1 cells and the ability of chaperones to rescue the conformation of these mutations. Expression of mutations in mammalian cells increased the median activity 16-fold and the amount of tetramers 3.2-fold compared with expression in bacteria. Subsequently, we tested the responses of seven selected mutations to three compounds with chaperone-like activity. Aminooxyacetic acid and 4-phenylbutyric acid exhibited only a weak effect. In contrast, heme arginate substantially increased the formation of mutant CBS protein tetramers (up to sixfold) and rescued catalytic activity (up to ninefold) of five out of seven mutations (p.A114V, p.K102N, p.R125Q, p.R266K, and p.R369C). The greatest effect of heme arginate was observed for the mutation p.R125Q, which is non-responsive to in vivo treatment with vitamin B(6). Moreover, the heme responsiveness of the p.R125Q mutation was confirmed in fibroblasts derived from a patient homozygous for this genetic variant. Based on these data, we propose that a distinct group of heme-responsive CBS mutations may exist and that the heme pocket of CBS may become an important target for designing novel therapies for homocystinuria.

  10. CBS and CSE are critical for maintenance of mitochondrial function and glucocorticoid production in adrenal cortex.

    Science.gov (United States)

    Wang, Chang-Nan; Liu, Yu-Jian; Duan, Guo-Li; Zhao, Wei; Li, Xiao-Han; Zhu, Xiao-Yan; Ni, Xin

    2014-12-01

    Mitochondria are known to play a central role in adrenocortical steroidogenesis. Recently, hydrogen sulfide (H2S), a gaseous transmitter endogenously produced by cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), has been found to improve mitochondrial function. The present study aimed at examining whether CBS and CSE are expressed in adrenal glands, and investigated the role of these enzymes in the maintenance of mitochondrial function and the production of glucocorticoids in adrenocortical cells. Both CBS and CSE are present in murine adrenocortical cells and account for H2S generation in adrenal glands. Using a combination of both in vivo and in vitro approaches, we demonstrated that either CBS/CSE inhibitors or small interfering RNAs led to mitochondrial oxidative stress and dysfunction, which meanwhile resulted in blunted corticosterone responses to adrenocorticotropic hormone (ACTH). These effects were significantly attenuated by the treatment of H2S donor GYY4137. Lipopolysaccharide (LPS) also caused mitochondrial damage, thereby resulting in adrenal insufficiency. Moreover, LPS inhibited CBS/CSE expression and H2S production in adrenal glands, while H₂S donor GYY4137 protected against LPS-induced mitochondrial damage and hyporesponsiveness to ACTH. Local suppression of CBS or CSE in adrenal glands significantly increased the mortality in endotoxemic mice, which was also improved by GYY4137. The identification of endogenous H2S generation as critical regulators of adrenocortical responsiveness might result in the development of new therapeutic approaches for the treatment of relative adrenal insufficiency during sepsis. Endogenous H₂S plays a critical role in the maintenance of mitochondrial function in the adrenal cortex, thereby resulting in an adequate adrenocortical response to ACTH.

  11. Extension of the composite CBS-QB3 method to singlet diradical calculations

    Science.gov (United States)

    Sirjean, Baptiste; Fournet, René; Glaude, Pierre-Alexandre; Ruiz-López, Manuel F.

    2007-02-01

    The composite CBS-QB3 method is widely used to obtain accurate energies of molecules and radicals although its use in the case of singlet diradicals gives rise to some difficulties. The problem is related to the parameterized correction this method introduces to account for spin-contamination. We report a new term specifically designed to describe singlet diradicals separated by at least one CH 2 unit. As a test case, we have computed the formation enthalpy of a series of diradicals that includes hydrocarbons as well as systems involving heteroatoms (nitrogen, oxygen). The resulting CBS-QB3 energies are very close to experiment.

  12. MP2/CBS atomic and molecular benchmarks for H through Ar

    Science.gov (United States)

    Barnes, Ericka C.; Petersson, George A.

    2010-03-01

    We extrapolate to the MP2/CBS limit with a sequence of optimized n-tuple-ζ augmented polarized basis sets (n =4, 5, 6, and 7) for the entire set of 72 atoms, positive and negative atomic ions, homonuclear diatomic molecules, and hydrides representing the first two rows of the Periodic Table. The second-order correlation energies agree with accurate (±0.01 mEh) numerical values (He, Be, Ne, Mg, Ar, Zn+2, and Kr) to within ±0.1%. These MP2/CBS limits of the 72 species can now be used as benchmarks to calibrate more approximate calculations using smaller basis sets.

  13. Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress

    Directory of Open Access Journals (Sweden)

    Qingnan Hao

    2016-04-01

    Full Text Available Nitrogen is an important macronutrient required for plant growth, and is a limiting factor for crop productivity. Improving the nitrogen use efficiency (NUE is therefore crucial. At present, the NUE mechanism is unclear and information on the genes associated with NUE in soybeans is lacking. cystathionine beta synthase (CBS domain-containing proteins (CDCPs may be implicated in abiotic stress tolerance in plants. We identified and classified a CBS domain–containing protein superfamily in soybean. A candidate gene for NUE, GmCBS21, was identified. GmCBS21 gene characteristics, the temporal expression pattern of the GmCBS21 gene, and the phenotype of GmCBS21 overexpression in transgenic Arabidopsis thaliana under low nitrogen stress were analyzed. The phenotypes suggested that the transgenic Arabidopsis thaliana seedlings performed better under the nitrogen-deficient condition. GmCBS21-overexpressing transgenic plants exhibit higher low nitrogen stress tolerance than WT plants, and this suggests its role in low nitrogen stress tolerance in plants. We conclude that GmCBS21 may serve as an excellent candidate for breeding crops with enhanced NUE and better yield.

  14. Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress.

    Science.gov (United States)

    Hao, Qingnan; Shang, Weijuan; Zhang, Chanjuan; Chen, Haifeng; Chen, Limiao; Yuan, Songli; Chen, Shuilian; Zhang, Xiaojuan; Zhou, Xinan

    2016-04-26

    Nitrogen is an important macronutrient required for plant growth, and is a limiting factor for crop productivity. Improving the nitrogen use efficiency (NUE) is therefore crucial. At present, the NUE mechanism is unclear and information on the genes associated with NUE in soybeans is lacking. cystathionine beta synthase (CBS) domain-containing proteins (CDCPs) may be implicated in abiotic stress tolerance in plants. We identified and classified a CBS domain-containing protein superfamily in soybean. A candidate gene for NUE, GmCBS21, was identified. GmCBS21 gene characteristics, the temporal expression pattern of the GmCBS21 gene, and the phenotype of GmCBS21 overexpression in transgenic Arabidopsis thaliana under low nitrogen stress were analyzed. The phenotypes suggested that the transgenic Arabidopsis thaliana seedlings performed better under the nitrogen-deficient condition. GmCBS21-overexpressing transgenic plants exhibit higher low nitrogen stress tolerance than WT plants, and this suggests its role in low nitrogen stress tolerance in plants. We conclude that GmCBS21 may serve as an excellent candidate for breeding crops with enhanced NUE and better yield.

  15. Nitrotyrosinylation, Remodeling and Endothelial-Myocyte Uncoupling in iNOS, Cystathionine Beta Synthase (CBS) Knockouts and iNOS/CBS Double Knockout Mice

    Science.gov (United States)

    Kundu, Soumi; Kumar, Munish; Sen, Utpal; Mishra, Paras K.; Tyagi, Neetu; Metreveli, Naira; Lominadze, David; Rodriguez, Walter; Tyagi, Suresh C.

    2009-01-01

    Increased levels of homocysteine (Hcy), recognized as hyperhomocysteinemia (HHcy), were associated with cardiovascular diseases. There was controversy regarding the detrimental versus cardio protective role of inducible nitric oxide synthase (iNOS) in ischemic heart disease. The aim of this study was to test the hypothesis that the Hcy generated nitrotyrosine by inducing the endothelial nitric oxide synthase, causing endothelial-myocyte (E–M) coupling. To differentiate the role of iNOS versus constitutive nitric oxide synthase (eNOS and nNOS) in Hcy-mediated nitrotyrosine generation and matrix remodeling in cardiac dysfunction, left ventricular (LV) tissue was analyzed from cystathionine beta synthase (CBS) heterozygote knockout, iNOS homozygote knockout, CBS−/+/iNOS−/− double knockout, and wild-type (WT) mice. The levels of nitrotyrosine, MMP-2 and -9 (zymographic analysis), and fibrosis (by trichrome stain) were measured. The endothelial-myocyte function was determined in cardiac rings. In CBS−/+ mice, homocysteine was elevated and in iNOS−/− mice, nitric oxide was significantly reduced. The nitrotyrosine and matrix metalloproteinase-9 (MMP-9) levels were elevated in double knockout and CBS−/+ as compared to WT mice. Although MMP-2 levels were similar in CBS−/+, iNOS−/−, and CBS−/+/iNOS−/−, the levels were three- to fourfold higher than WT. The levels of collagen were similar in CBS−/+ and iNOS−/−, but they were threefold higher than WT. Interesting, the levels of collagen increased sixfold in double knockouts, compared to WT, suggesting synergism between high Hcy and lack of iNOS. Left ventricular hypertrophy was exaggerated in the iNOS−/− and double knockout, and mildly increased in the CBS−/+, compared to WT mice. The endothelial-dependent relaxation was attenuated to the same extent in the CBS−/+ and iNOS−/−, compared to WT, but it was robustly blunted in double knockouts. The results concluded that homocysteine

  16. The ^12 C(,)^16 O Nuclear Reaction Rate from Asteroseismology of the DBV White Dwarf CBS 114

    Science.gov (United States)

    Metcalfe, T. S.; Handler, G.

    We have identified seven independent pulsation modes in the helium-atmosphere variable (DBV) white dwarf star CBS 114, based on 65 hours of time-resolved CCD photometry from the 0.75-m telescope at SAAO. We interpret these pulsations as non-radial g-modes with the same spherical degree l=1, as suggested by the mean period spacing of 37.1+/-0.7 seconds. We use a genetic-algorithm-based fitting method to find the globally optimal model parameters, including the stellar mass (M_* = 0.73 M_sun), the effective temperature (T_eff = 21,000 K), the mass of the atmospheric helium layer (log[M_He/M_*] = -6.66), and the central oxygen mass fraction (X_O = 0.61). The latter value implies a rate for the ^12C(alpha,gamma)^16O reaction near S_300 = 180 keV b, consistent with laboratory measurements.

  17. An artificial compressibility CBS method for modelling heat transfer and fluid flow in heterogeneous porous materials

    CSIR Research Space (South Africa)

    Malan, AG

    2011-08-01

    Full Text Available This work is concerned with the development of an artificial compressibility version of the characteristicbased split (CBS) method proposed by Zienkiewicz and Codina (Int. J. Numer. Meth. Fluids 1995; 20:869–885). The technique is applied...

  18. CBS-QB3 calculation of quantum chemical molecular descriptors of isomeric thiadiazoles.

    Science.gov (United States)

    Glossman-Mitnik, Daniel

    2006-12-01

    The results of the calculation of several molecular descriptors of isomeric thiadiazoles through the CBS-QB3 model chemistry are presented in this work. The results could be useful in quantitative structure-activity relationship (QSAR) or quantitative structure-property relationship (QSPR) studies of derivatives of the nitrogen-containing analogs of thiophene.

  19. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

    DEFF Research Database (Denmark)

    Pel, Herman J.; de Winde, Johannes H.; Archer, David B.

    2007-01-01

    The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level...

  20. Draft Genome Sequence of the Animal and Human Pathogen Malassezia pachydermatis Strain CBS 1879

    NARCIS (Netherlands)

    Triana, Sergio; González, Andrés; Ohm, Robin A|info:eu-repo/dai/nl/304837628; Wosten, Han|info:eu-repo/dai/nl/120693186; de Cock, Hans|info:eu-repo/dai/nl/087737116; Restrepo, Silvia; Celis, Adriana

    2015-01-01

    Malassezia pachydermatis is a basidiomycetous yeast that causes infections in humans and animals. Here, we report the genome sequence of Malassezia pachydermatis strain CBS 1879, which will facilitate the study of mechanisms underlying pathogenicity of the only non-lipid-dependent Malasezzia

  1. Draft Genome Sequence of the Yeast Pachysolen tannophilus CBS 4044/NRRL Y-2460

    DEFF Research Database (Denmark)

    Liu, Xiaoying; Kaas, Rolf Sommer; Jensen, Peter Ruhdal

    2012-01-01

    A draft genome sequence of the yeast Pachysolen tannophilus CBS 4044/NRRL Y-2460 is presented. The organism has the potential to be developed as a cell factory for biorefineries due to its ability to utilize waste feedstocks. The sequenced genome size was 12,238,196 bp, consisting of 34 scaffolds...

  2. Genome Sequence of the Yeast Clavispora lusitaniae Type Strain CBS 6936.

    Science.gov (United States)

    Durrens, Pascal; Klopp, Christophe; Biteau, Nicolas; Fitton-Ouhabi, Valérie; Dementhon, Karine; Accoceberry, Isabelle; Sherman, David J; Noël, Thierry

    2017-08-03

    Clavispora lusitaniae, an environmental saprophytic yeast belonging to the CTG clade of Candida, can behave occasionally as an opportunistic pathogen in humans. We report here the genome sequence of the type strain CBS 6936. Comparison with sequences of strain ATCC 42720 indicates conservation of chromosomal structure but significant nucleotide divergence. Copyright © 2017 Durrens et al.

  3. Draft Genome of Debaryomyces fabryi CBS 789T, Isolated from a Human Interdigital Mycotic Lesion.

    Science.gov (United States)

    Tafer, Hakim; Sterflinger, Katja; Lopandic, Ksenija

    2016-02-04

    The yeast genus Debaryomyces comprises species isolated from various natural habitats, man-made environments, and clinical materials. Here, the draft genome of D. fabryi CBS 789(T), isolated from a human interdigital mycotic lesion, is presented. Copyright © 2016 Tafer et al.

  4. Draft Genome Sequence of the Animal and Human Pathogen Malassezia pachydermatis Strain CBS 1879.

    Science.gov (United States)

    Triana, Sergio; González, Andrés; Ohm, Robin A; Wösten, Han A B; de Cock, Hans; Restrepo, Silvia; Celis, Adriana

    2015-10-15

    Malassezia pachydermatis is a basidiomycetous yeast that causes infections in humans and animals. Here, we report the genome sequence of Malassezia pachydermatis strain CBS 1879, which will facilitate the study of mechanisms underlying pathogenicity of the only non-lipid-dependent Malasezzia species. Copyright © 2015 Triana et al.

  5. A convenient enantioselective CBS-reduction of arylketones in flow-microreactor systems.

    Science.gov (United States)

    De Angelis, Sonia; De Renzo, Maddalena; Carlucci, Claudia; Degennaro, Leonardo; Luisi, Renzo

    2016-05-04

    A convenient, versatile, and green CBS-asymmetric reduction of aryl and heteroaryl ketones has been developed by using the microreactor technology. The study demonstrates that it is possible to handle borane solution safely within microreactors and that the reaction performs well using 2-MeTHF as a greener solvent.

  6. Complete Genome Sequences of the Xylose-Fermenting Candida intermedia Strains CBS 141442 and PYCC 4715.

    Science.gov (United States)

    Moreno, Antonio D; Tellgren-Roth, Christian; Soler, Lucile; Dainat, Jacques; Olsson, Lisbeth; Geijer, Cecilia

    2017-04-06

    Sustainable biofuel production from lignocellulosic materials requires efficient and complete use of all abundant sugars in the biomass, including xylose. Here, we report on the de novo genome assemblies of two strains of the xylose-fermenting yeast Candida intermedia: CBS 141442 and PYCC 4715. Copyright © 2017 Moreno et al.

  7. Cystathionine β-Synthase (CBS) Domain-containing Pyrophosphatase as a Target for Diadenosine Polyphosphates in Bacteria.

    Science.gov (United States)

    Anashkin, Viktor A; Salminen, Anu; Tuominen, Heidi K; Orlov, Victor N; Lahti, Reijo; Baykov, Alexander A

    2015-11-13

    Among numerous proteins containing pairs of regulatory cystathionine β-synthase (CBS) domains, family II pyrophosphatases (CBS-PPases) are unique in that they generally contain an additional DRTGG domain between the CBS domains. Adenine nucleotides bind to the CBS domains in CBS-PPases in a positively cooperative manner, resulting in enzyme inhibition (AMP or ADP) or activation (ATP). Here we show that linear P(1),P(n)-diadenosine 5'-polyphosphates (ApnAs, where n is the number of phosphate residues) bind with nanomolar affinity to DRTGG domain-containing CBS-PPases of Desulfitobacterium hafniense, Clostridium novyi, and Clostridium perfringens and increase their activity up to 30-, 5-, and 7-fold, respectively. Ap4A, Ap5A, and Ap6A bound noncooperatively and with similarly high affinities to CBS-PPases, whereas Ap3A bound in a positively cooperative manner and with lower affinity, like mononucleotides. All ApnAs abolished kinetic cooperativity (non-Michaelian behavior) of CBS-PPases. The enthalpy change and binding stoichiometry, as determined by isothermal calorimetry, were ~10 kcal/mol nucleotide and 1 mol/mol enzyme dimer for Ap4A and Ap5A but 5.5 kcal/mol and 2 mol/mol for Ap3A, AMP, ADP, and ATP, suggesting different binding modes for the two nucleotide groups. In contrast, Eggerthella lenta and Moorella thermoacetica CBS-PPases, which contain no DRTGG domain, were not affected by ApnAs and showed no enthalpy change, indicating the importance of the DTRGG domain for ApnA binding. These findings suggest that ApnAs can control CBS-PPase activity and hence affect pyrophosphate level and biosynthetic activity in bacteria. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Benchmarking Compound Methods (CBS-QB3, CBS-APNO, G3, G4, W1BD) against the Active Thermochemical Tables: Formation Enthalpies of Radicals.

    Science.gov (United States)

    Somers, Kieran P; Simmie, John M

    2015-08-20

    The 298.15 K formation enthalpies of 38 radicals with molecular formula CxHyOz have been computed via the atomization procedure using the five title methods. The computed formation enthalpies are then benchmarked against the values recommended in the Active Thermochemical Tables (ATcT). The accuracy of the methods have been interpreted in terms of descriptive statistics, including the mean-signed error, mean-unsigned error, maximum average deviation, 2σ uncertainties, and 2×root-mean-square-deviations (2RMSD). The results highlight the following rank order of accuracy for the methods studied G4 > G3 > W1BD > CBS-APNO > CBS-QB3. The findings of this work are also considered in light of a recent companion study, which took an identical approach to quantifying the accuracies of these methods for 48 closed-shell singlet CxHyOz compounds. A similar order of accuracies and precisions were observed therein: G3 > G4 > W1BD > CBS-APNO > CBS-QB3. Both studies highlight systematic biases/deviations from the ATcT for the methods investigated, which are discussed in some detail, with methods having clear tendencies to over- or underpredict the recommended formation enthalpies for radical and/or closed-shell CxHyOz compounds. We show that one can improve the accuracy of their computation, and simultaneously reduce the uncertainty, by taking unweighted average formation enthalpies from various combinations of methods used. The reader should note that the statistical analyses preceding these conclusions also highlight that these error cancellation effects are unique for closed-shell and radical species. By extension, these error-cancellation effects can be expected to be different for various homologous series and chemical functionalities and their closed- and open-shell subgroups. Hence, further benchmarking studies are advised for other homologous series, such that the scientists and engineers (e.g., combustion/atmospheric/astrochemical) who frequently use these methods can

  9. Methodology for breakdown of the CBS statistics for domestic natural gas and electricity consumption and historical trends; Methodiek voor opsplitsing CBS-statistiek huishoudelijk gas- en elektriciteitsverbruik en historisch trends

    Energy Technology Data Exchange (ETDEWEB)

    Tigchelaar, C. [ECN Beleidsstudies, Petten (Netherlands)

    2013-12-15

    From 2014 on Statistics Netherlands (CBS) is obliged to provide more detailed data on domestic energy consumption to the European statistical office Eurostat. It concerns a breakdown of the use of specific energy sources for space heating, water heating, cooking, cooling, lighting and other appliances [Dutch] Het CBS moet vanaf 2014 meer detailgegevens over het huishoudelijk energiegebruik aanleveren aan het Europees statistisch bureau Eurostat. Het gaat om een opsplitsing van het gebruik van specifieke energiedragers voor ruimteverwarming, warm tapwater, koken, koelen, verlichting en overige apparaten. ECN heeft het CBS ondersteund bij het ontwikkelen van een methode om deze opsplitsing te maken.

  10. THE ADAPTATION OF COACHING BEHAVIOR SCALE FOR SPORT (CBS-S) INTO TURKISH: A VALIDITY AND RELIABILITY STUDY

    OpenAIRE

    YAPAR, Ahmet; İNCE, Mustafa Levent

    2016-01-01

    The aim of the study was to test the construct validity and reliability of the Turkish version of Coaching Behavior Scale for Sport (CBS-S; Côté, et al., 1999). Totally, 171 men (%57,8) and 125 women (%42,2) athletes (n=296) aged between 14-22 years old completed the Turkish version of CBS-S. Participants were from Basketball (n=124, %42), Swimming (n=59, %20), Badminton (n=58, %19) and Track and Field Athletics (n=55, %19). CBS-S includes 47 items and 7 sub-dimensions. Each item is rated in ...

  11. Simplification of the CBS-QB3 method for predicting gas-phase deprotonation free energies

    Science.gov (United States)

    Casasnovas, Rodrigo; Frau, Juan; Ortega-Castro, Joaquín; Salvà, Antoni; Donoso, Josefa; Muñoz, Francisco

    Simplified versions of CBS-QB3 model chemistry were used to calculate the free energies of 36 deprotonation reactions in the gas phase. The best such version, S9, excluded coupled cluster calculation [CCSD(T)], and empirical (ΔEemp) and spin-orbit (ΔEint) correction terms. The mean absolute deviation and root mean square thus obtained (viz. 1.24 and 1.56 kcal/mol, respectively) were very-close to those provided by the original CBS-QB3 method (1.19 and 1.52 kcal/mol, respectively). The high-accuracy of the proposed simplification and its computational expeditiousness make it an excellent choice for energy calculations on gas-phase deprotonation reactions in complex systems.

  12. Abundances in the hot DZ star CBS 127 - How efficient is hydrogen screening

    Energy Technology Data Exchange (ETDEWEB)

    Sion, E.M.; Hammond, G.L.; Wagner, R.M.; Starrfield, S.G.; Liebert, J. (Villanova Univ., PA (USA) South Florida Univ., Tampa, FL (USA) Ohio State Univ., Columbus (USA) Arizona State Univ., Tempe (USA) Steward Observatory, Tucson, AZ (USA))

    1990-10-01

    A revised and updated grid of cool helium-rich model atmospheres which includes the effect of varying hydrogen abundance is used to analyze the temperature and abundance of the DZ white dwarf CBS 127. Comparisons of the star to several other DZ white dwarfs with similar T(e)s show that CBS 137 is the hottest DZ star and lies near the boundary temperature above which it has been proposed that hydrogen accretion may be prevented. Based on the results, a specifically defined single lower boundary temperature for screening is rejected, and it is demonstrated that the propeller must operate over a very broad range of T(eff), down to T(eff) as low as 5800 K, with highly variable efficiency. 39 refs.

  13. CBS 78 - A second extremely rare DBZ degenerate star with accreted calcium

    Science.gov (United States)

    Sion, E. M.; Shipman, H. L.; Wagner, R. M.; Starrfield, S. G.; Liebert, J.

    1986-09-01

    The paper reports the discovery of a second, extremely rare DBZ degenerate CBS 78 which reveals photospheric calcium in its nearly pure helium atmosphere. The presence of calcium strongly suggests accretion of interstellar matter as its origin, but hydrogen is not detected. The properties of CBS 78 are compared with other cool helium-rich degenerates with metals. The suggested existence of an empirical correlation between the hydrogen-to-calcium abundance ratio and effective temperature is confirmed. This correlation shows that above a critical temperature of about 11,000 K, a screening mechanism is operative which prevents accretion of interstellar hydrogen onto white dwarfs; this mechanism apparently does not operate at lower temperatures.

  14. Extension of the composite CBS-QB3 method to singlet diradical calculations.

    OpenAIRE

    Sirjean, Baptiste; Fournet, René; Glaude, Pierre-Alexandre; Ruiz-Lopez, M. F.

    2007-01-01

    International audience; The composite CBS-QB3 method is widely used to obtain accurate energies of molecules and radicals although its use in the case of singlet diradicals gives rise to some difficulties. The problem is related to the parameterized correction this method introduces to account for spin-contamination. We report a new term specifically designed to describe singlet diradicals separated by at least one CH2 unit. As a test case, we have computed the formation enthalpy of a series ...

  15. Simultaneous saccharification and fermentation (SSF) using cellobiose fermenting yeast Brettanomyces custersii (CBS 5512)

    Energy Technology Data Exchange (ETDEWEB)

    Spindler, D.D.; Grohmann, K.; Wyman, C.E.

    1991-01-16

    A process for producing ethanol from plant biomass includes forming a substrate from the biomass with the substrate including hydrolysates of cellulose and hemicellulose. A species of the yeast Brettanomyces custersii (CBS 5512), which has the ability to ferment both cellobiose and glucose to ethanol, is then selected and isolated. The substrate is inoculated with this yeast, and the inoculated substrate is then fermented under conditions favorable for cell viability and conversion of hydrolysates to ethanol.

  16. Peroxyoxalate-chemiluminescence of Tinopal CBS as a commercially important optical brightener: Mechanistic study and quantification

    Energy Technology Data Exchange (ETDEWEB)

    Shamsipur, Mojtaba, E-mail: mshamsipur@yahoo.co [Department of Chemistry, Razi University, Kermanshah (Iran, Islamic Republic of); Zargoosh, Kiomars [Department of Chemistry, Tarbiat Modares University, Tehran (Iran, Islamic Republic of); Javad Chaichi, Mohammad; Tajbakhsh, Mahmood; Parach, Ali [Department of Chemistry, Mazandaran University, Babolsar (Iran, Islamic Republic of)

    2010-05-15

    The peroxyoxalate-chemiluminescence arising from reaction of bis(2,4,6-trichlorophenyl)oxalate with hydrogen peroxide in the presence of a brightener Tinopal CBS (2,2'-((1,1'-biphenyl)-4,4'-diyldi-2.1-ethenediyl)bisbenzene sulfonic acid, disodium salt) has been studied. The relationship between the chemiluminescence intensity and concentrations of bis(2,4,6-trichlorophenyl)oxalate, sodium salicylate (as catalyst), hydrogen peroxide and Tinopal CBS is reported. The chemiluminescence parameters including intensity at maximum chemiluminescence, time at maximum intensity, total light yield, theoretical maximum level of intensity and pseudo-first-order rate constants for the rise and fall of the chemiluminescence burst (k{sub r} and k{sub f}) were evaluated from computer fitting of the resulting intensity-time plots. The activation parameters E{sub a}, DELTAH, DELTAS and DELTAG for the rise and fall steps were evaluated from the temperature dependence of k{sub r} and k{sub f} values. The results were discussed in terms of chemically initiated electron transfer between a reactive intermediate and Tinopal CBS as fluorescence activator. A possible mechanism involving dioxetanone derivatives as intermediates is proposed. Since there is a linear relationship between reciprocal of chemiluminescence intensity and reciprocal of fluorescer concentration, an analytical method based on partial least squares (PLS) regression was proposed for quantitative determination of Tinopal CBS. Satisfactory results were obtained with percent relative prediction error (RPE%) of 2.52 and detection limit of 2.7x10{sup -5} M.

  17. Draft Genome Sequence of Lachancea lanzarotensis CBS 12615T, an Ascomycetous Yeast Isolated from Grapes.

    Science.gov (United States)

    Sarilar, Véronique; Devillers, Hugo; Freel, Kelle C; Schacherer, Joseph; Neuvéglise, Cécile

    2015-04-16

    We report the genome sequencing of the yeast Lachancea lanzarotensis CBS 12615(T). The assembly comprises 24 scaffolds, for a total size of 11.46 Mbp. The annotation revealed 5,058 putative protein-coding genes. Detection of seven centromeres supports a chromosome fusion, which occurred after divergence from Lachancea thermotolerans and Lachancea kluyveri. Copyright © 2015 Sarilar et al.

  18. Draft Genome Sequence of the Mycoparasitic Oomycete Pythium oligandrum Strain CBS 530.74.

    Science.gov (United States)

    Kushwaha, Sandeep K; Vetukuri, Ramesh R; Grenville-Briggs, Laura J

    2017-05-25

    The oomycete Pythium oligandrum is a mycoparasite and licenced biological control agent. Here, we report the draft genome sequence of P. oligandrum strain CBS 530.74, which is 36.80 Mb. It contains 341 scaffolds and 11,647 predicted protein-coding genes. As reported for plant-pathogenic Pythium species, RXLR-type effector sequences are absent. Copyright © 2017 Kushwaha et al.

  19. Draft Genome Sequence of Sporidiobolus salmonicolor CBS 6832, a Red-Pigmented Basidiomycetous Yeast.

    Science.gov (United States)

    Coelho, Marco A; Almeida, João M G C F; Hittinger, Chris Todd; Gonçalves, Paula

    2015-05-21

    We report the genome sequencing and annotation of the basidiomycetous red-pigmented yeast Sporidiobolus salmonicolor strain CBS 6832. The current assembly contains 395 scaffolds, for a total size of about 20.5 Mb and a G+C content of ~61.3%. The genome annotation predicts 5,147 putative protein-coding genes. Copyright © 2015 Coelho et al.

  20. CCSD(T)/CBS fragment-based calculations of lattice energy of molecular crystals

    Science.gov (United States)

    Červinka, Ctirad; Fulem, Michal; Růžička, Květoslav

    2016-02-01

    A comparative study of the lattice energy calculations for a data set of 25 molecular crystals is performed using an additive scheme based on the individual energies of up to four-body interactions calculated using the coupled clusters with iterative treatment of single and double excitations and perturbative triples correction (CCSD(T)) with an estimated complete basis set (CBS) description. The CCSD(T)/CBS values on lattice energies are used to estimate sublimation enthalpies which are compared with critically assessed and thermodynamically consistent experimental values. The average absolute percentage deviation of calculated sublimation enthalpies from experimental values amounts to 13% (corresponding to 4.8 kJ mol-1 on absolute scale) with unbiased distribution of positive to negative deviations. As pair interaction energies present a dominant contribution to the lattice energy and CCSD(T)/CBS calculations still remain computationally costly, benchmark calculations of pair interaction energies defined by crystal parameters involving 17 levels of theory, including recently developed methods with local and explicit treatment of electronic correlation, such as LCC and LCC-F12, are also presented. Locally and explicitly correlated methods are found to be computationally effective and reliable methods enabling the application of fragment-based methods for larger systems.

  1. CO-CBS-H2 S Axis: From Vascular Mediator to Cancer Regulator.

    Science.gov (United States)

    Suematsu, Makoto; Nakamura, Takashi; Tokumoto, Yasuhito; Yamamoto, Takehiro; Kajimura, Mayumi; Kabe, Yasuaki

    2016-04-01

    CO is a gaseous mediator generated by HO. Our previous studies revealed that CO generated from inducible HO-1 or from constitutive HO-2 modulates function of different heme proteins or enzymes through binding to their prosthetic ferrous heme to alter their structures, regulating biological function of cells and organs. Such CO-directed target macromolecules include sGC and CBS. In the liver, CO serves as a sinusoidal dilator through its action on sGC in hepatic stellate cells, while the same gas accounts for vasoconstrictor that inhibits H2S generated by CO-sensitive CBS in astrocytes. Since molecular O2 is a substrate for HO, the latter mechanism contributes to hypoxic vasodilation in neurovascular units. We have recently uncovered that stress-inducible CO in and around cancer cells suppresses CBS to result in decreased methylation of PFKFB3, the enzyme regulating PFK-1, leading to a shift of glucose biotransformation from glycolysis toward pentose phosphate pathway; such a metabolic remodeling causes chemoresistance through increasing NADPH and reduced glutathione under stress conditions for cancer cells. This article reviews the intriguing networks of CO-sensitive metabolic regulatory mechanisms in microcirculation and cancer. © 2015 John Wiley & Sons Ltd.

  2. Sequences of metanicins, 20-residue peptaibols from the ascomycetous fungus CBS 597.80.

    Science.gov (United States)

    Kimonyo, Anastase; Brückner, Hans

    2013-05-01

    Four linear 20-residue peptaibols, named metanicins (MTCs) A-D, were isolated from submerged cultures of the ascomycetous fungus CBS 597.80. Structure elucidation was performed by a combination of fast-atom-bombardment mass spectrometry (FAB-MS), electrospray ionization MS, Edman degradation of isolated fragments, and amino acid analysis by ion-exchange and gas chromatography, and enantioselective HPLC. The sequences of MTC A(B) are (amino acid exchange in B and C in parentheses): Ac-Aib-Ala-Aib-Ala-Aib-Ala-Gln-Aib-Val-Aib-Gly-Leu-Aib-Pro-Val-Aib-Aib(D-Iva)-Gln-Gln-Pheol and of MTC C(D) Ac-Aib-Ala-Aib-Ala-Aib-Ala-Gln-Aib-Val-Aib-Gly-Leu-Aib-Pro-Val-Aib-Aib(D-Iva)-Gln-Gln-Pheol (Ac, acetyl; Aib, α-aminoisobutyric acid; Iva, isovaline; Pheol, L-phenylalaninol). The peptides are related, and some of the sequences are identical, to other 20-residue peptaibols isolated from Trichoderma species. MTCs show moderate activities against Micrococcus luteus, Enterococcus faecalis, and Staphylococcus aureus, and very low activities against Bacillus subtilis. The producer has originally been identified and deposited as Metarhizium anisopliae var. anisopliae CBS 597.80. Although this identification has been withdrawn by Centralbureau voor Schimmelcultures (CBS) in the meantime, the accession number will be retained - independently from any taxonomic revisions. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.

  3. ANALISIS PENGARUH KESUKSESAN IMPLEMENTASI CORE BANKING SYSTEM (CBS DENGAN BERBASIS MODEL DELONE DAN MCLEAN

    Directory of Open Access Journals (Sweden)

    Mardiana Andarwati

    2016-10-01

    Full Text Available AbstractCore Banking System (CBS is banking application system implementation to improve service customers, but whether the bank knew that CBS was categorized applied successfully or not.The purpose of theseresearch are determine the successful implementation of CBS using models DeLone and Mclean IS Success consisting of six variables are system quality , information quality, usage , user satisfaction , individual impact , and impact organizations. Test the hypothesis using the Partial Least Suare (PLS.The results of this study are the quality of the system on the intensity of use and user satisfaction are positive and significant,the quality of information on the intensity of use is positive and significant and then user satisfaction isnegative and not significant, the influence of the intensity of use of the employment impacts of individual positive and significant, user satisfaction to the impact of individual positivel and significant, the intensity of use of the user satisfaction the positive and significant, and the impact individual on the impact of the organizationis positive and significant. So, as to impact individual the best relationship of the impact organization 

  4. Genome Sequence of Type Strain Fonsecaea multimorphosa CBS 980.96T, a Causal Agent of Feline Cerebral Phaeohyphomycosis

    Science.gov (United States)

    Leao, Aniele C. Ribas; Weiss, Vinicius Almir; Vicente, Vania Aparecida; Costa, Flavia; Bombassaro, Amanda; Raittz, Roberto Tadeu; Steffens, Maria Berenice R.; Pedrosa, Fabio Oliveira; Gomes, Renata R.; Baura, Valter; Faoro, Helisson; Sfeir, Michelle Zibetti Tadra; Balsanelli, Eduardo; Moreno, Leandro F.; Najafzadeh, M. Javad

    2017-01-01

    ABSTRACT A draft genome sequence of type strain Fonsecaea multimorphosa CBS 980.96T was obtained. This species was first isolated from a cat with cerebral phaeohyphomycosis in Queensland, Australia. PMID:28209838

  5. Genome Sequence of Type Strain Fonsecaea multimorphosa CBS 980.96(T), a Causal Agent of Feline Cerebral Phaeohyphomycosis.

    Science.gov (United States)

    Leao, Aniele C Ribas; Weiss, Vinicius Almir; Vicente, Vania Aparecida; Costa, Flavia; Bombassaro, Amanda; Raittz, Roberto Tadeu; Steffens, Maria Berenice R; Pedrosa, Fabio Oliveira; Gomes, Renata R; Baura, Valter; Faoro, Helisson; Sfeir, Michelle Zibetti Tadra; Balsanelli, Eduardo; Moreno, Leandro F; Najafzadeh, M Javad; de Hoog, Sybren; Souza, Emanuel Maltempi

    2017-02-16

    A draft genome sequence of type strain Fonsecaea multimorphosa CBS 980.96(T) was obtained. This species was first isolated from a cat with cerebral phaeohyphomycosis in Queensland, Australia. Copyright © 2017 Leao et al.

  6. Boryl substitution of acetaldehyde makes it an enol: inconsistency between Gn/CBS and ab initio/DFT data.

    Science.gov (United States)

    Balabin, Roman M

    2010-03-18

    Tautomerism, a particular case of isomerism, plays an important role in modern organic chemistry, biochemistry, medicinal chemistry, pharmacology, and molecular biology. Inconsistency between results of complex energy computation methods Gn/CBS (G2, G3, CBS-4M, and CBS-QB3) and high-level ab initio/DFT ones (CCSD(T)/CBS, MP2/CBS, and B3LYP/aug-cc-pVTZ) is found. Gn/CBS methods provide a qualitatively different description of tautomeric (keto-enol) equilibrium in 2-substituted acetaldehydes. According to valence focal point analysis (FPA) based on CCSD(T)/aug-cc-pVTZ, MP3/aug-cc-pVQZ, and MP2/aug-cc-pV5Z energies, boryl substitution of acetaldehyde makes it an enol. In other words, enol was found to be the global minimum on the potential energy surface (PES) of C(2)H(5)BO. Gn/CBS methods predict the keto form to be the minimum. The relative energy of alkenol, CH(BH(2))=CH(OH), is calculated to be -1.67 +/- 0.82 kcal mol(-1) at CCSD(T)/CBS level of theory. Hydrogen shift effects are also calculated in two other 2-substituted acetaldehydes, namely, 3-oxopropanenitrile (C(3)H(3)NO) and ethanal (C(2)H(4)O), with a general formula of XH(2)C-CHO (X = BH(2), CN, and H). Electron density (charge) transfer between the C=C double bond and the free p orbital of the boron atom (B) in a boryl group (BH(2)) greatly stabilizes enol with respect to ketone, CH(2)(BH(2))-CHO. The first known stabilization of enol in an acetaldehyde derivative, without an intramolecular hydrogen bond (H-bond), questions the accuracy of complex energy computation methods for boron-containing molecules. The possible reasons and consequences of this finding are discussed.

  7. DNA hypomethylation of CBS promoter induced by folate deficiency is a potential noninvasive circulating biomarker for colorectal adenocarcinomas.

    Science.gov (United States)

    Xue, Geng; Lu, Chao-Jing; Pan, Shu-Jun; Zhang, Yin-Ling; Miao, Hui; Shan, Shi; Zhu, Xiao-Ting; Zhang, Yi

    2017-08-01

    Aberrant DNA methylation patterns, which induced by folate deficiency, play important roles in tumorigenesis of colorectal cancer (CRC). Some DNA methylation alterations can also be detected in cell-free DNA (cfDNA) of patients' plasma, making cfDNA an ideal noninvasive circulating biomarker. However, exact DNA methylation alterations induced by folate deficiency in tumorigenesis of CRC and exact potential circulating cfDNA methylation biomarker are still unclear. Therefore, DNA methylation patterns of the normal human colon mucosal epithelial cell line (NCM460), cultured with normal or low folate content, were screened and the DNA hypomethylation of cystathionine-beta-synthase (CBS) promoter was further validated in vitro and vivo. Then, the correlation analysis between folate level, DNA methylation alteration in promoter and expression of CBS was carried out in vitro and vivo. Further, the methylation patterns of CBS promoter in plasma cfDNA were detected and statistically correlated with pathological parameters and clinical outcome. Our study showed that DNA hypomethylation in CBS promoter, induced by folate deficiency, would lead to up-regulation of CBS both in vitro and vivo. Patients with cfDNA hypomethylation of CBS promoter in plasma were correlated with high tumor stage and poor clinical outcome. In addition, cfDNA hypomethylation of CBS promoter in plasma was shown to be an independent prognostic factor for recurrence and cancer-related death in CRC. Our results indicated that DNA hypomethylation of CBS promoter induced by folate deficiency could serve as a potential noninvasive circulating biomarker and may be helpful in developing more effective prognostic markers for CRC.

  8. Separation of polychlorinated dibenzo-p-dioxins/furans, non-ortho/mono/di/tri/tetra-ortho-polychlorinated biphenyls, and polybrominated diphenyl ethers groups of compounds prior to their determination with large volume injection gas chromatography-Quadrupole ion storage tandem mass spectrometry.

    Science.gov (United States)

    Roszko, Marek; Szymczyk, Krystyna; Jędrzejczak, Renata

    2013-10-17

    Polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are important environmental contaminants. Their maximum legally allowable levels in food and environment are in the low pg g(-1) range. Therefore some highly selective and sensitive analytical methods must be used to determine them. Prior to final determination by GC/MS the cleaned-up samples have to be split into some fractions in view of large differences in concentrations of various analytes and existence of numerous chromatographic co-elutions (which in any case cannot be fully avoided). The aim of this study was to: (i) develop a robust, time-saving analytical method to isolate, clean-up and fractionate PCBs, PBDEs and PCDD/Fs prior to their determination with gas chromatography/ion trap mass spectrometry; (ii) assess method performance using laboratory validation data and some certified reference materials; (iii) use the developed method to assess PCB/PBDE/PCDD/F levels in samples of commercially available food. A combination of alumina, florisil, modified silica gel and two carbon columns were used for sample cleanup and fractionation. Separate fractions containing dioxins/furans, PBDE, non-ortho, mono-ortho and di-/tri-/tetra-ortho PCBs were obtained. The method statistical parameters were compatible with 1883/2006 EC Regulation (80-120%, RSD below 15%). The method performance was successfully used to evaluation of some real life food samples. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Improving cyanobacterail O2-tolerance using CBS hydrogenase for hydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Maness, Pin-Ching [National Renewable Energy Lab. (NREL), Golden, CO (United States); Eckert, Carrie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Wawrousek, Karen [National Renewable Energy Lab. (NREL), Golden, CO (United States); Noble, Scott [National Renewable Energy Lab. (NREL), Golden, CO (United States); Pennington, Grant [National Renewable Energy Lab. (NREL), Golden, CO (United States); Yu, Jianping [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-11-11

    Cyanobacterial H2 production is a viable path to renewable H2 with water serving as the electron donor and sunlight the energy source. A grand challenge is the sensitivity of the underlying hydrogenase to O2, the latter an inherent byproduct of oxygenic photosynthesis. This challenge has been identified as a technical barrier in the Fuel Cell Technologies Office (FCTO) Multi-year Research, Development and Deployment Plan. One solution is to express in cyanobacterium an O2-tolerant hydrogenase to circumvent this barrier. We have uncovered an O2-tolerant hydrogenase from a photosynthetic bacterium Rubrivivax gelatinosus CBS (Casa Bonita Strain; hereafter “CBS”) with a half-life near 21 h when exposed to ambient O2. We sequenced the CBS genome and identified two sets of maturation machineries hyp1 and hyp2. Transcripts expression analysis and mutagenesis revealed that hyp1 is responsible for the assembly of the O2-tolerant CO-oxidation (Coo) hydrogenase and hyp2 is involved in the maturation of a H2-uptake hydrogenase. The structural genes encoding the O2-tolerant hydrogenase (cooLXUH) and maturation genes hyp1FABCDE were therefore cloned and expressed in the model cyanobacterium Synechocystis sp. PCC 6803. We obtained several recombinants displaying hydrogenase activity in a Synechocystis host lacking background activity, suggesting that the CBS hydrogenase is active in Synechocystis. Yet the activity is extremely low. To ensure balanced protein expression, we systematically optimized heterologous expression of 10 CBS genes by using stronger promoters and better ribosome binding site. Moreover we attempted the expression of cooM and cooK genes, verified to be important in CBS to afford activity. CooM is a very large protein and both CooM and CooK are membrane-associated. These properties limited our success in expressing both genes in Synechocystis, although they

  10. Serum cystathionine β-synthase (CBS, cystathionine γ-lyase (CSE and cytochrome c oxidase (COX in copper mine miners potentially expose d to hydrogen sulfide

    Directory of Open Access Journals (Sweden)

    Anna Skoczyńska

    2015-08-01

    Full Text Available Background: The aim of the study was to evaluate serum levels of the target enzyme for H2S toxicity – cytochrome c oxidase (COX and enzymes involved in the synthesis of H2S – cystathionine β-synthase (CBS and cystathionine γ-lyase (CSE in copper mine miners. Material and Methods: The initial and basic study was conducted respectively in 237 and 88 miners, working in 2 mining shafts: I – no H2S emissions recorded in the last 10 years (study group A and II – H2S emissions occurred (study group B. A medical examination was performed and 10 ml of blood was collected from miners immediately after exiting the mine. Results: There were no clinical or biochemical changes typical for H2S toxicity. Sulfhemoglobine was undetectable and there were no changes in the red-ox system. However, in group B, regulatory changes were found; a tendency to higher concentration of CBS and CSE, a higher activity of angiotensin converting enzyme (ACE compared to group A (p < 0.05 and a linear relationship between ACE and CSE (r = 0.6927; p < 0.001. It has been shown that cigarette smoking decreases COX (p < 0.05, however, in miners working in shaft II, the decreased level of COX may result also from the presence of H2S in the gaseous emissions. Conclusions: COX concentration can be a sensitive indicator of exposure to H2S. The measurements of blood H2S concentrations carried out in workplaces should explain the cause of the changes observed in the COX, CBS and CSE activity. Med Pr 2015;66(4:539–548

  11. Hydrogen sulfide generation in mammals: the molecular biology of cystathionine-β- synthase (CBS) and cystathionine-γ-lyase (CSE).

    Science.gov (United States)

    Renga, Barbara

    2011-04-01

    Cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) are two key enzymes involved in the synthesis of hydrogen sulphide (H(2)S). CBS catalyzes the pyridoxal 5'-phosphate (PLP)-dependent conversion of homocysteine in Cystathionine whilst CSE the pyridoxal 5'-phosphate (PLP)-dependent synthesis of L-cysteine from Cystathionine. In mammals, CBS gene transcription is poorly investigated and the activity of the enzyme is highly regulated. In fact, the CBS enzyme contains a heme cofactor that functions as a redox sensor and utilizes S-adenosylmethionine (SAM) as an allosteric activator. Impaired CBS activity causes hyperhomocystinuria and hyperhomocysteinemia, both risk factors for cardiovascular diseases. Murine CSE gene regulation is well characterized but little is known about the human counterpart and there is no information regarding the enzyme activity regulation. Recently it has been demonstrated that CSE transcription is regulated by the nuclear receptor Farnesoid X Receptor (FXR). Mutations that decrease the activity of CSE cause cystathioninuria, hypercystathioninemia and increase the risk of developing atherosclerosis and bladder cancer. This review focuses on the recent aspects of the molecular regulation of both CBS and CSE and highlights the possibility that members of the nuclear receptors superfamily might be involved in the regulation of hydrogen sulphide metabolism.

  12. Serotonin and dopamine protect from hypothermia/rewarming damage through the CBS/H2S pathway.

    Science.gov (United States)

    Talaei, Fatemeh; Bouma, Hjalmar R; Van der Graaf, Adrianus C; Strijkstra, Arjen M; Schmidt, Martina; Henning, Robert H

    2011-01-01

    Biogenic amines have been demonstrated to protect cells from apoptotic cell death. Herein we show for the first time that serotonin and dopamine increase H(2)S production by the endogenous enzyme cystathionine-β-synthase (CBS) and protect cells against hypothermia/rewarming induced reactive oxygen species (ROS) formation and apoptosis. Treatment with both compounds doubled CBS expression through mammalian target of rapamycin (mTOR) and increased H(2)S production in cultured rat smooth muscle cells. In addition, serotonin and dopamine treatment significantly reduced ROS formation. The beneficial effect of both compounds was minimized by inhibition of their re-uptake and by pharmacological inhibition of CBS or its down-regulation by siRNA. Exogenous administration of H(2)S and activation of CBS by Prydoxal 5'-phosphate also protected cells from hypothermic damage. Finally, serotonin and dopamine pretreatment of rat lung, kidney, liver and heart prior to 24 h of hypothermia at 3°C followed by 30 min of rewarming at 37°C upregulated the expression of CBS, strongly reduced caspase activity and maintained the physiological pH compared to untreated tissues. Thus, dopamine and serotonin protect cells against hypothermia/rewarming induced damage by increasing H(2)S production mediated through CBS. Our data identify a novel molecular link between biogenic amines and the H(2)S pathway, which may profoundly affect our understanding of the biological effects of monoamine neurotransmitters.

  13. Mg2+-dependent Interactions of ATP with the Cystathionine-β-Synthase (CBS) Domains of a Magnesium Transporter*

    Science.gov (United States)

    Hirata, Yusuke; Funato, Yosuke; Takano, Yu; Miki, Hiroaki

    2014-01-01

    Ancient conserved domain protein/cyclin M (CNNM) family proteins are evolutionarily conserved Mg2+ transporters. However, their biochemical mechanism of action remains unknown. Here, we show the functional importance of the commonly conserved cystathionine-β-synthase (CBS) domains and reveal their unique binding ability to ATP. Deletion mutants of CNNM2 and CNNM4, lacking the CBS domains, are unable to promote Mg2+ efflux. Furthermore, the substitution of one amino acid residue in the CBS domains of CNNM2, which is associated with human hereditary hypomagnesemia, abrogates Mg2+ efflux. Binding analyses reveal that the CBS domains of CNNM2 bind directly to ATP and not AMP in a manner dependent on the presence of Mg2+, which is inhibited in a similar pattern by the disease-associated amino acid substitution. The requirement of Mg2+ for these interactions is a unique feature among CBS domains, which can be explained by the presence of highly electronegative surface potentials around the ATP binding site on CNNM2. These results demonstrate that the CBS domains play essential roles in Mg2+ efflux, probably through interactions with ATP. Interactions with ATP, which mostly forms complexes with Mg2+ in cells, may account for the rapid Mg2+ transport by CNNM family proteins. PMID:24706765

  14. Mg2+-dependent interactions of ATP with the cystathionine-β-synthase (CBS) domains of a magnesium transporter.

    Science.gov (United States)

    Hirata, Yusuke; Funato, Yosuke; Takano, Yu; Miki, Hiroaki

    2014-05-23

    Ancient conserved domain protein/cyclin M (CNNM) family proteins are evolutionarily conserved Mg(2+) transporters. However, their biochemical mechanism of action remains unknown. Here, we show the functional importance of the commonly conserved cystathionine-β-synthase (CBS) domains and reveal their unique binding ability to ATP. Deletion mutants of CNNM2 and CNNM4, lacking the CBS domains, are unable to promote Mg(2+) efflux. Furthermore, the substitution of one amino acid residue in the CBS domains of CNNM2, which is associated with human hereditary hypomagnesemia, abrogates Mg(2+) efflux. Binding analyses reveal that the CBS domains of CNNM2 bind directly to ATP and not AMP in a manner dependent on the presence of Mg(2+), which is inhibited in a similar pattern by the disease-associated amino acid substitution. The requirement of Mg(2+) for these interactions is a unique feature among CBS domains, which can be explained by the presence of highly electronegative surface potentials around the ATP binding site on CNNM2. These results demonstrate that the CBS domains play essential roles in Mg(2+) efflux, probably through interactions with ATP. Interactions with ATP, which mostly forms complexes with Mg(2+) in cells, may account for the rapid Mg(2+) transport by CNNM family proteins. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  15. Serotonin and dopamine protect from hypothermia/rewarming damage through the CBS/H2S pathway.

    Directory of Open Access Journals (Sweden)

    Fatemeh Talaei

    Full Text Available Biogenic amines have been demonstrated to protect cells from apoptotic cell death. Herein we show for the first time that serotonin and dopamine increase H(2S production by the endogenous enzyme cystathionine-β-synthase (CBS and protect cells against hypothermia/rewarming induced reactive oxygen species (ROS formation and apoptosis. Treatment with both compounds doubled CBS expression through mammalian target of rapamycin (mTOR and increased H(2S production in cultured rat smooth muscle cells. In addition, serotonin and dopamine treatment significantly reduced ROS formation. The beneficial effect of both compounds was minimized by inhibition of their re-uptake and by pharmacological inhibition of CBS or its down-regulation by siRNA. Exogenous administration of H(2S and activation of CBS by Prydoxal 5'-phosphate also protected cells from hypothermic damage. Finally, serotonin and dopamine pretreatment of rat lung, kidney, liver and heart prior to 24 h of hypothermia at 3°C followed by 30 min of rewarming at 37°C upregulated the expression of CBS, strongly reduced caspase activity and maintained the physiological pH compared to untreated tissues. Thus, dopamine and serotonin protect cells against hypothermia/rewarming induced damage by increasing H(2S production mediated through CBS. Our data identify a novel molecular link between biogenic amines and the H(2S pathway, which may profoundly affect our understanding of the biological effects of monoamine neurotransmitters.

  16. Theoretical Calculation of Jet Fuel Thermochemistry. 1; Tetrahydrodicylopentadiene (JP10) Thermochemistry Using the CBS-QB3 and G3(MP2)//B3LYP Methods

    Science.gov (United States)

    Zehe, Michael J.; Jaffe, Richard L.

    2010-01-01

    High-level ab initio calculations have been performed on the exo and endo isomers of gas-phase tetrahydrodicyclopentadiene (THDCPD), a principal component of the jet fuel JP10, using the Gaussian Gx and Gx(MPx) composite methods, as well as the CBS-QB3 method, and using a variety of isodesmic and homodesmotic reaction schemes. The impetus for this work is to help resolve large discrepancies existing between literature measurements of the formation enthalpy Delta (sub f)H deg (298) for exo-THDCPD. We find that use of the isodesmic bond separation reaction C10H16 + 14CH4 yields 12C2H6 yields results for the exo isomer (JP10) in between the two experimentally accepted values, for the composite methods G3(MP2), G3(MP2)//B3LYP, and CBS-QB3. Application of this same isodesmic bond separation scheme to gas-phase adamantane yields a value for Delta (sub f)H deg (298) within 5 kJ/mol of experiment. Isodesmic bond separation calculations for the endo isomer give a heat of formation in excellent agreement with the experimental measurement. Combining our calculated values for the gas-phase heat of formation with recent measurements of the heat of vaporization yields recommended values for Delta (sub f)H deg (298)liq of -126.4 and -114.7 kJ/mol for the exo and endo isomers, respectively.

  17. UTC Power/Delphi SECA CBS Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gorman, Michael; Kerr, Rich

    2013-04-04

    The subject report summarizes the results of solid oxide fuel cell development conducted by UTC Power in conjunction with Delphi Automotive Systems under a cost-share program with from October 2008 through March of 2013. Over that period Delphi Automotive Systems developed a nearly four times larger area solid oxide fuel cell stack capable of operating on pre-reformed natural gas and simulated coal gas with durability demonstrated to 5,000 hours and projected to exceed 10,000 hours. The new stack design was scaled to 40-cell stacks with power output in excess of 6.25kW. Delphi also made significant strides in improving the manufacturability, yield and production cost of these solid oxide fuel cells over the course of the program. Concurrently, UTC Power developed a conceptual design for a 120 MW Integrated Gasification Fuel Cell (IGFC) operating on coal syngas with as high as 57% Higher Heating Value (HHV) efficiency as a measure of the feasibility of the technology. Subsequently a 400 kW on-site system preliminary design with 55% Lower Heating Value (LHV) efficiency operating on natural gas was down-selected from eighteen candidate designs. That design was used as the basis for a 25kW breadboard power plant incorporating four Delphi cell stacks that was tested on natural gas before the program was discontinued due to the sale of UTC Power in early 2013. Though the program was cut short of the endurance target of 3,000 hours, many aspects of the technology were proven including: large-area, repeatable cell manufacture, cell stack operation on simulated coal gas and natural gas and integrated power plant operation on natural gas. The potential of the technology for high efficiency stationary electric power generation is clear. Acceptable production costs, durability, and reliability in real world environments are the remaining challenges to commercialization.

  18. A Database of Formation Enthalpies of Nitrogen Species by Compound Methods (CBS-QB3, CBS-APNO, G3, G4).

    Science.gov (United States)

    Simmie, John M

    2015-10-22

    Accurate thermochemical data for compounds containing C/H/N/O are required to underpin kinetics simulation and modeling of the reactions of these species in different environments. There is a dearth of experimental data so computational quantum chemistry has stepped in to fill this breach and to verify whether particular experiments are in need of revision. A number of composite model chemistries (CBS-QB3, CBS-APNO, G3, and G4) are used to compute theoretical atomization energies and hence enthalpies of formation at 0 and 298.15 K, and these are benchmarked against the best available compendium of values, the Active Thermochemical Tables or ATcT. In general the agreement is very good for some 28 species with the only discrepancy being for hydrazine. It is shown that, although individually the methods do not perform that well, collectively the mean unsigned error is <1.7 kJ mol(-1); hence, this approach provides a useful tool to screen published values and validate new experimental results. Using multiple model chemistries does have some drawbacks but can produce good results even for challenging molecules like HOON and CN2O2. The results for these smaller validated molecules are then used as anchors for determining the formation enthalpies of larger species such as methylated hydrazines and diazenes, five- and six-membered heterocyclics via carefully chosen isodesmic working reactions with the aim of resolving some discrepancies in the literature and establishing a properly validated database. This expanded database could be useful in testing the performance of computationally less-demanding density function methods with newer functionals that have the capacity to treat much larger systems than those tested here.

  19. centrosomin's beautiful sister (cbs) encodes a GRIP-domain protein that marks Golgi inheritance and functions in the centrosome cycle in Drosophila.

    Science.gov (United States)

    Eisman, Robert C; Stewart, Natasha; Miller, David; Kaufman, Thomas C

    2006-08-15

    The mechanism of inheritance of the Golgi complex is an important problem in cell biology. In this study, we examine the localization and function of a Golgi protein encoded by centrosomin's beautiful sister (cbs) during cleavage in Drosophila melanogaster. Cbs contains a GRIP domain that is 57% identical to vertebrate Golgin-97. Cbs undergoes a dramatic relocalization during mitosis from the cytoplasm to an association with chromosomes from late prometaphase to early telophase, by a transport mechanism that requires the GRIP domain and Arl1, the product of the Arf72A locus. Additionally, Cbs remains independent of the endoplasmic reticulum throughout cleavage. The use of RNAi, Arf72A mutant analysis and ectopic expression of the GRIP domain, shows that cycling of Cbs during mitosis is required for the centrosome cycle. The effects on the centrosome cycle depend on Cbs concentration and Cbs transport from the cytoplasm to DNA. When Cbs levels are reduced centrosomes fail to mature, and when Cbs transport is impeded by ectopic expression of the GRIP domain, centrosomes undergo hypertrophy. We propose that, Cbs is a trans-Golgi protein that links Golgi inheritance to the cell cycle and the Drosophila Golgi is more vertebrate-like than previously recognized.

  20. The association between the 844ins68 polymorphism in the CBS gene and breast cancer.

    Science.gov (United States)

    Gallegos-Arreola, Martha Patricia; Figuera-Villanueva, Luis Eduardo; Ramos-Silva, Adriana; Salas-González, Efraín; Puebla-Pérez, Ana María; Peralta-Leal, Valeria; García-Ortiz, José Elías; Dávalos-Rodríguez, Ingrid Patricia; Zúñiga-González, Guillermo Moisés

    2014-12-22

    The cystathionine beta synthase (CBS) gene plays an important role in homocysteine metabolism because it catalyzes the first step of the transsulfuration pathway, during which homocysteine is converted to cystathionine. Polymorphisms of CBS have been associated with cancer. We examined the role of the 844ins68 polymorphism by comparing the genotypes of 371 healthy Mexican women with the genotypes of 323 Mexican women with breast cancer (BC). The observed genotype frequencies for controls and BC patients were 1% and 2% for Ins/Ins, 13% and 26% for W/Ins, and 86% and 72% for W/W, respectively. We found that the odds ratio (OR) was 2.2, with a 95% confidence interval (95% CI) of 1.5-3.3, p = 0.0001. The association was also evident when comparing the distribution of the W/Ins-Ins/Ins genotypes in patients in the following categories: 1) menopause and high γ-glutamyltransferase (GGT) levels (OR of 2.17, 95% CI: 1.17-4.26, p = 0.02), 2) chemotherapy response and high lactate dehydrogenase (LDH) levels (OR 2.2, 95% CI: 1.08-4.4, p = 0.027), 3) chemotherapy response and high GGT levels (OR 2.46, 95% CI: 1.2-4.8, p = 0.007), and 4) body mass index (BMI) and III-IV tumor stage (OR 3.2, 95% CI: 1.2-8.3, p = 0.013). We conclude that the genotypes W/Ins-Ins/Ins of the 844ins68 polymorphism in the CBS gene contribute significantly to BC susceptibility in the analyzed sample from the Mexican population.

  1. The association between the 844ins68 polymorphism in the CBS gene and breast cancer

    Science.gov (United States)

    Figuera-Villanueva, Luis Eduardo; Ramos-Silva, Adriana; Salas-González, Efraín; Puebla-Pérez, Ana María; Peralta-Leal, Valeria; García-Ortiz, José Elías; Dávalos-Rodríguez, Ingrid Patricia; Zúñiga-González, Guillermo Moisés

    2014-01-01

    Introduction The cystathionine beta synthase (CBS) gene plays an important role in homocysteine metabolism because it catalyzes the first step of the transsulfuration pathway, during which homocysteine is converted to cystathionine. Polymorphisms of CBS have been associated with cancer. Material and methods We examined the role of the 844ins68 polymorphism by comparing the genotypes of 371 healthy Mexican women with the genotypes of 323 Mexican women with breast cancer (BC). Results The observed genotype frequencies for controls and BC patients were 1% and 2% for Ins/Ins, 13% and 26% for W/Ins, and 86% and 72% for W/W, respectively. We found that the odds ratio (OR) was 2.2, with a 95% confidence interval (95% CI) of 1.5–3.3, p = 0.0001. The association was also evident when comparing the distribution of the W/Ins-Ins/Ins genotypes in patients in the following categories: 1) menopause and high γ-glutamyltransferase (GGT) levels (OR of 2.17, 95% CI: 1.17–4.26, p = 0.02), 2) chemotherapy response and high lactate dehydrogenase (LDH) levels (OR 2.2, 95% CI: 1.08–4.4, p = 0.027), 3) chemotherapy response and high GGT levels (OR 2.46, 95% CI: 1.2–4.8, p = 0.007), and 4) body mass index (BMI) and III–IV tumor stage (OR 3.2, 95% CI: 1.2–8.3, p = 0.013). Conclusions We conclude that the genotypes W/Ins-Ins/Ins of the 844ins68 polymorphism in the CBS gene contribute significantly to BC susceptibility in the analyzed sample from the Mexican population. PMID:25624861

  2. Polymorphisms in MTHFR, MS and CBS genes and homocysteine levels in a Pakistani population.

    Science.gov (United States)

    Yakub, Mohsin; Moti, Naushad; Parveen, Siddiqa; Chaudhry, Bushra; Azam, Iqbal; Iqbal, Mohammad Perwaiz

    2012-01-01

    Hyperhomocysteinemia (>15 µmol/L) is highly prevalent in South Asian populations including Pakistan. In order to investigate the genetic determinants of this condition, we studied 6 polymorphisms in genes of 3 enzymes--methylenetetrahydrofolate reductase (MTHFR; C677T; A1298C), methionine synthase (MS; A2756G), cystathionine-β-synthase (CBS; T833C/844ins68, G919A) involved in homocysteine metabolism and investigated their interactions with nutritional and environmental factors in a Pakistani population. In a cross-sectional survey, 872 healthy adults (355 males and 517 females; age 18-60 years) were recruited from a low-income urban population in Karachi. Fasting venous blood was obtained and assessed for plasma/serum homocysteine; folate, vitamin B12, pyridoxal phosphate and blood lead. DNA was isolated and genotyping was performed by PCR-RFLP (restriction-fragment-length-polymorphism) based assays. The average changes in homocysteine levels for MTHFR 677CT and TT genotypes were positive [β(SE β), 2.01(0.63) and 16.19(1.8) µmol/L, respectively]. Contrary to MTHFR C677T polymorphism, the average changes in plasma homocysteine levels for MS 2756AG and GG variants were negative [β(SE β), -0.56(0.58) and -0.83(0.99) µmol/L, respectively]. The average change occurring for CBS 844ins68 heterozygous genotype (ancestral/insertion) was -1.88(0.81) µmol/L. The combined effect of MTHFR C677T, MS A2756G and CBS 844ins68 genotypes for plasma homocysteine levels was additive (p value CBS 844ins68 compared to homozygous ancestral type [OR (95% CI); 0.58 (0.34-0.99)]. Individuals with MTHFR 677CT or TT genotypes were at a greater risk of hyperhomocysteinemia in folate and vitamin B12 deficiencies and high blood lead (p value <0.05) level. Gene polymorphism (especially MTHFR C677T transition), folate and vitamin B12 deficiencies, male gender and high blood lead level appear to be contributing towards the development of hyperhomocysteinemia in a Pakistani population.

  3. Efter- og videreuddannelsesaktiviteter på FSKK på CBS

    Directory of Open Access Journals (Sweden)

    Henrik Køhler Simonsen

    2006-06-01

    ISSN 1603-5518. Den moderne vidensmedarbejder har behov for bedre og mere fleksibel efter- og videreuddannelse. Meget tyder på, at dette behov vil blive endnu mere udtalt, når efter- og videreuddannelse måske kommer på dagsordenen i forbindelse med næste års overenskomstforhandlinger. Artiklen diskuterer en række strategiske og praktiske overvejelser i forbindelse med udvikling af Efter- og videreuddannelsesområdet på Fakultetet for Sprog, Kultur og Kommunikation på CBS. Den giver bl.a. nogle konkrete forslag til modeller til udvikling og udbud af efter- og videreuddannelsesaktiviteter.

  4. CBS Genome Atlas Database: a dynamic storage for bioinformatic results and sequence data

    DEFF Research Database (Denmark)

    Hallin, Peter Fischer; Ussery, David

    2004-01-01

    , these results counts to more than 220 pieces of information. The backbone of this solution consists of a program package written in Perl, which enables administrators to synchronize and update the database content. The MySQL database has been connected to the CBS web-server via PHP4, to present a dynamic web...... content for users outside the center. This solution is tightly fitted to existing server infrastructure and the solutions proposed here can perhaps serve as a template for other research groups to solve database issues....

  5. Polymorphisms in MTHFR, MS and CBS genes and homocysteine levels in a Pakistani population.

    Directory of Open Access Journals (Sweden)

    Mohsin Yakub

    Full Text Available BACKGROUND: Hyperhomocysteinemia (>15 µmol/L is highly prevalent in South Asian populations including Pakistan. In order to investigate the genetic determinants of this condition, we studied 6 polymorphisms in genes of 3 enzymes--methylenetetrahydrofolate reductase (MTHFR; C677T; A1298C, methionine synthase (MS; A2756G, cystathionine-β-synthase (CBS; T833C/844ins68, G919A involved in homocysteine metabolism and investigated their interactions with nutritional and environmental factors in a Pakistani population. METHODOLOGY/PRINCIPAL FINDINGS: In a cross-sectional survey, 872 healthy adults (355 males and 517 females; age 18-60 years were recruited from a low-income urban population in Karachi. Fasting venous blood was obtained and assessed for plasma/serum homocysteine; folate, vitamin B12, pyridoxal phosphate and blood lead. DNA was isolated and genotyping was performed by PCR-RFLP (restriction-fragment-length-polymorphism based assays. The average changes in homocysteine levels for MTHFR 677CT and TT genotypes were positive [β(SE β, 2.01(0.63 and 16.19(1.8 µmol/L, respectively]. Contrary to MTHFR C677T polymorphism, the average changes in plasma homocysteine levels for MS 2756AG and GG variants were negative [β(SE β, -0.56(0.58 and -0.83(0.99 µmol/L, respectively]. The average change occurring for CBS 844ins68 heterozygous genotype (ancestral/insertion was -1.88(0.81 µmol/L. The combined effect of MTHFR C677T, MS A2756G and CBS 844ins68 genotypes for plasma homocysteine levels was additive (p value <0.001. Odds of having hyperhomocysteinemia with MTHFR 677TT genotype was 10-fold compared to MTHFR 677CC genotype [OR (95%CI; 10.17(3.6-28.67]. Protective effect towards hyperhomocysteinemia was observed with heterozygous (ancestral/insertion genotype of CBS 844ins68 compared to homozygous ancestral type [OR (95% CI; 0.58 (0.34-0.99]. Individuals with MTHFR 677CT or TT genotypes were at a greater risk of hyperhomocysteinemia in folate and

  6. Mechanisms of hyperhomocysteinemia induced skeletal muscle myopathy after ischemia in the CBS-/+ mouse model.

    Science.gov (United States)

    Veeranki, Sudhakar; Tyagi, Suresh C

    2015-01-06

    Although hyperhomocysteinemia (HHcy) elicits lower than normal body weights and skeletal muscle weakness, the mechanisms remain unclear. Despite the fact that HHcy-mediated enhancement in ROS and consequent damage to regulators of different cellular processes is relatively well established in other organs, the nature of such events is unknown in skeletal muscles. Previously, we reported that HHcy attenuation of PGC-1α and HIF-1α levels enhanced the likelihood of muscle atrophy and declined function after ischemia. In the current study, we examined muscle levels of homocysteine (Hcy) metabolizing enzymes, anti-oxidant capacity and focused on protein modifications that might compromise PGC-1α function during ischemic angiogenesis. Although skeletal muscles express the key enzyme (MTHFR) that participates in re-methylation of Hcy into methionine, lack of trans-sulfuration enzymes (CBS and CSE) make skeletal muscles more susceptible to the HHcy-induced myopathy. Our study indicates that elevated Hcy levels in the CBS-/+ mouse skeletal muscles caused diminished anti-oxidant capacity and contributed to enhanced total protein as well as PGC-1α specific nitrotyrosylation after ischemia. Furthermore, in the presence of NO donor SNP, either homocysteine (Hcy) or its cyclized version, Hcy thiolactone, not only increased PGC-1α specific protein nitrotyrosylation but also reduced its association with PPARγ in C2C12 cells. Altogether these results suggest that HHcy exerts its myopathic effects via reduction of the PGC-1/PPARγ axis after ischemia.

  7. Galactose utilization sheds new light on sugar metabolism in the sequenced strain Dekkera bruxellensis CBS 2499.

    Science.gov (United States)

    Moktaduzzaman, Md; Galafassi, Silvia; Capusoni, Claudia; Vigentini, Ileana; Ling, Zhihao; Piškur, Jure; Compagno, Concetta

    2015-03-01

    Dekkera bruxellensis and Saccharomyces cerevisiae are considered two phylogenetically distant relatives, but they share several industrial relevant traits such as the ability to produce ethanol under aerobic conditions (Crabtree effect), high tolerance towards ethanol and acids, and ability to grow without oxygen. Beside a huge adaptability, D. bruxellensis exhibits a broader spectrum in utilization of carbon and nitrogen sources in comparison to S. cerevisiae. With the aim to better characterize its carbon source metabolism and regulation, the usage of galactose and the role that glucose plays on sugar metabolism were investigated in D. bruxellensis CBS 2499. The results indicate that in this yeast galactose is a non-fermentable carbon source, in contrast to S. cerevisiae that can ferment it. In particular, its metabolism is affected by the nitrogen source. Interestingly, D. bruxellensis CBS 2499 exhibits the 'short-term Crabtree effect', and the expression of genes involved in galactose utilization and in respiratory metabolism is repressed by glucose, similarly to what occurs in S. cerevisiae. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

  8. Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii

    Energy Technology Data Exchange (ETDEWEB)

    Fernández-Millán, Pablo; Kortazar, Danel; Lucas, María [Unidad de Cristalografía Macromolecular, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 800, 48160 Derio, Vizcaya (Spain); Martínez-Chantar, María Luz [Unidad de Metabolómica, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 801, 48160 Derio, Vizcaya (Spain); Astigarraga, Egoitz; Fernández, José Andrés [Departamento de Química-Física, Universidad del País Vasco UPV-EHU, Barrio Sarriena s/n, E-48940 Leioa (Spain); Sabas, Olatz [Unidad de Cristalografía Macromolecular, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 800, 48160 Derio, Vizcaya (Spain); Albert, Armando [Instituto de Química-Física ‘Rocasolano’, CSIC, c/Serrano 119, 28006 Madrid (Spain); Mato, Jose M. [Unidad de Metabolómica, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 801, 48160 Derio, Vizcaya (Spain); Martínez-Cruz, Luis Alfonso, E-mail: amartinez@cicbiogune.es [Unidad de Cristalografía Macromolecular, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 800, 48160 Derio, Vizcaya (Spain)

    2008-07-01

    Trigonal crystals of MJ0729 showing different degrees of merohedral twinning that may vary from perfect hemihedral twinning to perfect tetartohedral twinning were obtained upon slight variation of the pH. CBS domains are small protein motifs, usually associated in tandem, that are implicated in binding to adenosyl groups. Several genetic diseases in humans have been associated with mutations in CBS sequences, which has made them very promising targets for rational drug design. Trigonal crystals of the CBS-domain protein MJ0729 from Methanococcus jannaschii were grown by the vapour-diffusion method at acidic pH. Preliminary analysis of nine X-ray diffraction data sets using Yeates statistics and Britton plots showed that slight variation in the pH as well as in the buffer used in the crystallization experiments led to crystals with different degrees of merohedral twinning that may vary from perfect hemihedral twinning to perfect tetartohedral twinning.

  9. Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii

    Science.gov (United States)

    Fernández-Millán, Pablo; Kortazar, Danel; Lucas, María; Martínez-Chantar, María Luz; Astigarraga, Egoitz; Fernández, José Andrés; Sabas, Olatz; Albert, Armando; Mato, Jose M.; Martínez-Cruz, Luis Alfonso

    2008-01-01

    CBS domains are small protein motifs, usually associated in tandem, that are implicated in binding to adenosyl groups. Several genetic diseases in humans have been associated with mutations in CBS sequences, which has made them very promising targets for rational drug design. Trigonal crystals of the CBS-domain protein MJ0729 from Methanococcus jannaschii were grown by the vapour-diffusion method at acidic pH. Preliminary analysis of nine X-ray diffraction data sets using Yeates statistics and Britton plots showed that slight variation in the pH as well as in the buffer used in the crystallization experiments led to crystals with different degrees of merohedral twinning that may vary from perfect hemihedral twinning to perfect tetartohedral twinning. PMID:18607087

  10. Absorption, distribution, metabolism and excretion of N-cyclohexyl-2-benzothiazyl sulfenamide (CBS), a vulcanizing accelerator, in rats

    Energy Technology Data Exchange (ETDEWEB)

    Adachi, Tohru; Tanaka, Akira; Yamaha, Tsutomu (National Inst. of Hygienic Sciences, Tokyo (Japan))

    1989-05-01

    Absorption, distribution, metabolism and excretion were studied in rats following a single oral administration of N-cyclohexyl-2-benzothiazyl sulfenamide (CBS) at a dose of 250 mg/kg. About 65% and 24% of the dose were excreted into urine and feces, respectively, for 3 days after administration of labeled CBS (cyclohexyl-/sup 14/C). Biliary excretion amounted to about 5% of the dose for 3 days. While about 92% of the dose was recovered in urine and feces at a ratio of 1 : 1 within 3 days when /sup 14/C-2CBS was given. No specific organ-affinity was observed in distribution study. Cyclohexylamine and 2-mercaptobenzothiazole were identified as urinary metabolites. (author).

  11. Cystathionine β-synthase (CBS) domains confer multiple forms of Mg2+-dependent cooperativity to family II pyrophosphatases.

    Science.gov (United States)

    Salminen, Anu; Anashkin, Viktor A; Lahti, Matti; Tuominen, Heidi K; Lahti, Reijo; Baykov, Alexander A

    2014-08-15

    Regulated family II pyrophosphatases (CBS-PPases) contain a nucleotide-binding insert comprising a pair of cystathionine β-synthase (CBS) domains, termed a Bateman module. By binding with high affinity to the CBS domains, AMP and ADP usually inhibit the enzyme, whereas ATP activates it. Here, we demonstrate that AMP, ADP, and ATP bind in a positively cooperative manner to CBS-PPases from four bacteria: Desulfitobacterium hafniense, Clostridium novyi, Clostridium perfringens, and Eggerthella lenta. Enzyme interaction with substrate as characterized by the Michaelis constant (Km) also exhibited positive catalytic cooperativity that decreased in magnitude upon nucleotide binding. The degree of both types of cooperativity increased with increasing concentration of the cofactor Mg(2+) except for the C. novyi PPase where Mg(2+) produced the opposite effect on kinetic cooperativity. Further exceptions from these general rules were ADP binding to C. novyi PPase and AMP binding to E. lenta PPase, neither of which had any effect on activity. A genetically engineered deletion variant of D. hafniense PPase lacking the regulatory insert was fully active but differed from the wild-type enzyme in that it was insensitive to nucleotides and bound substrate non-cooperatively and with a smaller Km value. These results indicate that the regulatory insert acts as an internal inhibitor and confers dual positive cooperativity to CBS domain-containing PPases, making them highly sensitive regulators of the PPi level in response to the changes in cell energy status that control adenine nucleotide distribution. These regulatory features may be common among other CBS domain-containing proteins. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. CBS c.844ins68 Polymorphism Frequencies in Control Populations: Implications on Nonsyndromic Cleft Lip With or Without Cleft Palate.

    Science.gov (United States)

    Murthy, Jyotsna; Lakkakula, Saikrishna; Gurramkonda, Venkatesh Babu; Pathapati, Ram Mohan; Maram, Rajasekhar; Lakkakula, Bhaskar V K S

    2015-01-01

    Nonsyndromic cleft lip with or without cleft palate (NSCLP) is a common birth defect with substantial clinical and social impact. Folate deficiency is one of the factors that have been associated with increased risk for NSCLP. Polymorphisms in folate and homocysteine pathway genes may act as susceptibility factors. The objective of this study was to evaluate prevalence estimates of cystathionine beta-synthase (CBS) insertion of 68-bp (c.844ins68) polymorphisms and their correlation with NSCLP. A total of 236 unrelated individuals from seven Indian populations and an additional 355 cases with NSCLP and 357 controls without NSCLP were included in this study. We investigated the CBS c.844ins68 polymorphism in all samples. Genotyping was performed with polymerase chain reaction and electrophoresis. The data were statistically analyzed using the chi-square test. The CBS c.844ins68 allele is present in six of the seven populations analyzed, and allele frequencies range from 1.5% in Balija to 9.1% in Sugali populations. The CBS c.844ins68 polymorphism showed a significant protective effect on NSCLP at both genotype (WW versus WI: odds ratio [OR] = 0.54, 95% confidence interval [CI] = 0.31 to 0.95, P = .149) and allele levels (W versus I: OR = 0.56, 95% CI = 0.32 to 0.96, P = .033). The current study observed significant differences in the frequency of the CBS 844ins68 allele across populations. There is a significant association between CBS c.844ins68 polymorphism and cleft lip and palate in the Indian population. Additional studies are warranted to identify the functional variants in the genes controlling homocysteine as etiological contributors to the formation of oral clefts.

  13. Down-regulated CBS/H2S pathway is involved in high-salt-induced hypertension in Dahl rats.

    Science.gov (United States)

    Huang, Pan; Chen, Siyao; Wang, Yuan; Liu, Jia; Yao, Qiuyu; Huang, Yaqian; Li, Hongxia; Zhu, Mingzhu; Wang, Suxia; Li, Lin; Tang, Chaoshu; Tao, Yinghong; Yang, Guosheng; Du, Junbao; Jin, Hongfang

    2015-04-30

    The study was designed to explore the significance of endogenous H2S in the development of high-salt-induced hypertension in rats. High-salt-induced hypertension rat model was made by feeding Dahl rat high-salt diet containing 8% NaCl for 8 weeks with SD rats as control. SBP and aorta structure in rats were observed. Endogenous H2S content and expression of cystathionine β-lyase (CBS), cystathionine γ-lyase and mercaptopyruvate sulfurtransferase in renal tissues were detected. Mechanisms for the impact of high-salt on CBS/H2S in renal tissues were studied, targeting HIF-1α pathway. The effect of H2S on RAS in serum and renal tissue of rats were tested. High-salt reduced endogenous H2S content and inhibited the expression of CBS in renal tissue in salt-sensitive Dahl rats. H2S donor, however, inhibited salt-sensitive hypertension, reversed aortic structural remodeling and inhibited activation of the RAS system in renal tissues in Dahl rats. Expression of HIF-1α was decreased but expression of PHD2 was increased in renal tissue of Dahl rats with high-salt diet, whereas they did not alter in renal tissue of SD rats with high-salt diet. Ex vivo experiment showed that inhibitor of HIF-1α degradation could rescue down-regulated CBS/H2S pathway in renal tissue of Dahl rats with high-salt. In contrast, inhibitor of HIF-1α activity decreased the CBS/H2S pathway in the renal tissue of SD rats treated with high-salt. Down-regulated CBS/H2S pathway in renal tissues under high-salt insult might be an important pathogenesis of salt-sensitive hypertension. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Folding and activity of mutant cystathionine β-synthase depends on the position and nature of the purification tag: characterization of the R266K CBS mutant.

    Science.gov (United States)

    Majtan, Tomas; Kraus, Jan P

    2012-04-01

    Cystathionine β-synthase (CBS), a heme-containing pyridoxal-5-phosphate (PLP)-dependent enzyme, catalyzes the condensation of serine and homocysteine to yield cystathionine. Missense mutations in CBS, the most common cause of homocystinuria, often result in misfolded proteins. Arginine 266, where the pathogenic missense mutation R266K was identified, appears to be involved in the communication between heme and the PLP-containing catalytic center. Here, we assessed the effect of a short affinity tag (6xHis) compared to a bulky fusion partner (glutathione S-transferase - GST) on CBS wild type (WT) and R266K mutant enzyme properties. While WT CBS was successfully expressed either in conjunction with a GST or with a 6xHis tag, the mutant R266K CBS had no activity, did not form native tetramers and did not respond to chemical chaperone treatment when expressed with a GST fusion partner. Interestingly, expression of R266K CBS constructs with a 6xHis tag at either end yielded active enzymes. The purified, predominantly tetrameric, R266K CBS with a C-terminal 6xHis tag had ∼82% of the activity of a corresponding WT CBS construct. Results from thermal pre-treatment of the enzyme and the denaturation profile of R266K suggests a lower thermal stability of the mutant enzyme compared to WT, presumably due to a disturbed heme environment. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. Crystal structures of the CBS and DRTGG domains of the regulatory region of Clostridiumperfringens pyrophosphatase complexed with the inhibitor, AMP, and activator, diadenosine tetraphosphate.

    Science.gov (United States)

    Tuominen, H; Salminen, A; Oksanen, E; Jämsen, J; Heikkilä, O; Lehtiö, L; Magretova, N N; Goldman, A; Baykov, A A; Lahti, R

    2010-05-07

    Nucleotide-binding cystathionine beta-synthase (CBS) domains serve as regulatory units in numerous proteins distributed in all kingdoms of life. However, the underlying regulatory mechanisms remain to be established. Recently, we described a subfamily of CBS domain-containing pyrophosphatases (PPases) within family II PPases. Here, we express a novel CBS-PPase from Clostridium perfringens (CPE2055) and show that the enzyme is inhibited by AMP and activated by a novel effector, diadenosine 5',5-P1,P4-tetraphosphate (AP(4)A). The structures of the AMP and AP(4)A complexes of the regulatory region of C. perfringens PPase (cpCBS), comprising a pair of CBS domains interlinked by a DRTGG domain, were determined at 2.3 A resolution using X-ray crystallography. The structures obtained are the first structures of a DRTGG domain as part of a larger protein structure. The AMP complex contains two AMP molecules per cpCBS dimer, each bound to a single monomer, whereas in the activator-bound complex, one AP(4)A molecule bridges two monomers. In the nucleotide-bound structures, activator binding induces significant opening of the CBS domain interface, compared with the inhibitor complex. These results provide structural insight into the mechanism of CBS-PPase regulation by nucleotides. Copyright 2010 Elsevier Ltd. All rights reserved.

  16. Cystathionine beta-Synthase (CBS) Domains 1 and 2 Fulfill Different Roles in Ionic Strength Sensing of the ATP-binding Cassette (ABC) Transporter OpuA

    NARCIS (Netherlands)

    Karasawa, Akira; Erkens, Guus B.; Berntsson, Ronnie P. -A.; Otten, Renee; Schuurman-Wolters, Gea K.; Mulder, Frans A. A.; Poolman, Bert

    2011-01-01

    The cystathionine beta-synthase module of OpuA in conjunction with an anionic membrane surface acts as a sensor of internal ionic strength, which allows the protein to respond to osmotic stress. We now show by chemical modification and cross-linking studies that CBS2-CBS2 interface residues are crit

  17. Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

    NARCIS (Netherlands)

    Martínez, B.; Sillanpää, J.; Smit, E.; Korhonen, T.K.; Pouwels, P.H.

    2000-01-01

    The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting

  18. Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

    NARCIS (Netherlands)

    Martínez, B.; Sillanpää, J.; Smit, E.; Korhonen, T.K.; Pouwels, P.H.

    2000-01-01

    The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting sig

  19. Detailed kinetic study of the ring opening of cycloalkanes by CBS-QB3 calculations

    CERN Document Server

    Sirjean, Baptiste; Ruiz-Lopez, M F; Fournet, Ren{é}

    2006-01-01

    This work reports a theoretical study of the gas phase unimolecular decomposition of cyclobutane, cyclopentane and cyclohexane by means of quantum chemical calculations. A biradical mechanism has been envisaged for each cycloalkane, and the main routes for the decomposition of the biradicals formed have been investigated at the CBS-QB3 level of theory. Thermochemical data (\\delta H^0_f, S^0, C^0_p) for all the involved species have been obtained by means of isodesmic reactions. The contribution of hindered rotors has also been included. Activation barriers of each reaction have been analyzed to assess the 1 energetically most favorable pathways for the decomposition of biradicals. Rate constants have been derived for all elementary reactions using transition state theory at 1 atm and temperatures ranging from 600 to 2000 K. Global rate constant for the decomposition of the cyclic alkanes in molecular products have been calculated. Comparison between calculated and experimental results allowed to validate the ...

  20. Funding 3D-CBS:. Changing the Role of PET for Cancer Screening

    Science.gov (United States)

    Crosetto, Dario B.

    2010-04-01

    The role of Positron Emission Technology (PET) should be changed with use of the 30-CBS (Three Dimensional Complete Body screening) for maximizing the capture of signals that will detect minimum abnormal metabolism (or other biological processes), achievable by capturing simultaneously and accurately as many signals as possible from the tumor markers from all organs of the body in order to identify the smallest anomaly, at the lowest cost per signal captured and requiring the minimum radiation to the patient. This paper provides scientific arguments for setting new parameters for industry to establish the correct relation between the goal of obtaining substantial reduction in cancer deaths and the implementation of innovations and technology that will provide the expected results through early cancer detection.

  1. Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68.

    Science.gov (United States)

    Samadlouie, Hamid-Reza; Hamidi-Esfahani, Zohreh; Alavi, Seyed-Mehdi; Varastegani, Boshra

    2014-01-01

    The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.

  2. Open Science CBS Neuroimaging Repository: Sharing ultra-high-field MR images of the brain.

    Science.gov (United States)

    Tardif, Christine Lucas; Schäfer, Andreas; Trampel, Robert; Villringer, Arno; Turner, Robert; Bazin, Pierre-Louis

    2016-01-01

    Magnetic resonance imaging at ultra high field opens the door to quantitative brain imaging at sub-millimeter isotropic resolutions. However, novel image processing tools to analyze these new rich datasets are lacking. In this article, we introduce the Open Science CBS Neuroimaging Repository: a unique repository of high-resolution and quantitative images acquired at 7 T. The motivation for this project is to increase interest for high-resolution and quantitative imaging and stimulate the development of image processing tools developed specifically for high-field data. Our growing repository currently includes datasets from MP2RAGE and multi-echo FLASH sequences from 28 and 20 healthy subjects respectively. These datasets represent the current state-of-the-art in in-vivo relaxometry at 7 T, and are now fully available to the entire neuroimaging community. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Ethanol fermentation of red oak acid prehydrolysate by the yeast Pichia stipitis CBS 5776

    Energy Technology Data Exchange (ETDEWEB)

    Tran, A.V.; Chambers, R.P.

    1986-07-01

    Xylose, the dominant sugar in red oak acid prehydrolysate, was fermented to ethanol. In batch cultures of xylose concentrations ranging from 10 to 100 g/l, Pichia stipitis CBS 5776 yielded 0.50-0.40 g ethanol per g xylose consumed. Model compounds of inhibitors generated in the acid prehydrolysis of red oak hemicelluloses, lignin and extractives, hindered the fermentation. Recycled yeasts and treatments with molecular sieve or mixed bed ion resins facilitated the ethanol fermentation of red oak acid prehydrolysate. A maximal ethanol concentration of 9.9 g/l was obtained from an acid prehydrolysate containing 21.7 g/l of xylose. Fermentation inhibitors derived from red oak lignin and extractives were identified. 20 references.

  4. Efter- og videreuddannelsesaktiviteter på FSKK på CBS

    Directory of Open Access Journals (Sweden)

    Henrik Køhler Simonsen

    2006-05-01

    Full Text Available Første gang publiceret i UNEV nr. 8: Tilrettelæggelse af efter- og videreuddannelse på universitetet, nov. 2006, red. Sanne Almeborg og Tom Nyvang.
    ISSN 1603-5518.

    Den moderne vidensmedarbejder har behov for bedre og mere fleksibel efter- og videreuddannelse. Meget tyder på, at dette behov vil blive endnu mere udtalt, når efter- og videreuddannelse måske kommer på dagsordenen i forbindelse med næste års overenskomstforhandlinger. Artiklen diskuterer en række strategiske og praktiske overvejelser i forbindelse med udvikling af Efter- og videreuddannelsesområdet på Fakultetet for Sprog, Kultur og Kommunikation på CBS. Den giver bl.a. nogle konkrete forslag til modeller til udvikling og udbud af efter- og videreuddannelsesaktiviteter.

  5. Geosmin and Related Volatiles in Bioreactor-Cultured Streptomyces citreus CBS 109.60

    Science.gov (United States)

    Pollak, F. C.; Berger, R. G.

    1996-01-01

    Streptomyces citreus CBS 109.60 produced geosmin and a complex pattern of other volatile compounds during cultivation in a 2.5-liter laboratory bioreactor. Volatiles were isolated from disrupted cells, from the culture medium, and from the waste air of the bioreactor by adsorption on Lewatit OC 1064MD. Quantitative and qualitative analyses were carried out using capillary gas chromatography and coupled gas chromatography-mass spectroscopy. S. citreus produced 56 volatile compounds, which were mainly terpenoids but also included aliphatic ketones, alcohols, esters, pyrazines, furan(on)es, and aromatic types during the growth phase. The major components were geosmin and a germacradienol. A biosynthetic pathway for geosmin including eudesmanolides is proposed. PMID:16535293

  6. Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68

    Directory of Open Access Journals (Sweden)

    Hamid-Reza Samadlouie

    2014-06-01

    Full Text Available The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.

  7. Isolation of mutants of Talaromyces emersonii CBS 814. 70 with enhanced cellulase activity

    Energy Technology Data Exchange (ETDEWEB)

    Moloney, A.P.; Hackett, T.J.; Considine, P.J.; Coughlan, M.P.

    1983-07-01

    By a combination of genetic mutation and modification of growth medium the cellulase (see 1,4-(1,3,1,4)-beta-d-glucan 4-glucanohydrolase, EC 3.2 1.4 etc.) activity of culture filtrates of Talaromyces emersonii CBS 814.70 has been increased four-fold to approximately 2 U/ml and a productivity of 20-25 U/lh. At 50 degrees C this system was completely stable for at least 24 h. At 60 degrees C in static reaction mixtures 19% of the original activity was lost compared with 21% when mixtures were shaken. At 70 degrees C the loss of activity after 4 h was 64% without shaking and 70% when shaken. The cellulase system from Trichoderma reesei was decidedly less stable than that of Talaromyces emersonii under each of the above conditions. The ability of each enzyme system, separately and together, to digest beet pulp was investigated. (35 Refs.)

  8. Thermomyces lanuginosus CBS 395.62/b Strain as Rich Source of α-Galactosidase Enzyme

    Directory of Open Access Journals (Sweden)

    Quang D. Nguyen

    2003-01-01

    Full Text Available Seventeen Thermomyces lanuginosus strains, cultivated on raffinose and sucrose, were ranked on the basis of α-galactosidase activities. T. lanuginosus CBS 395.62/b strain showed the highest α-galactosidase activity on both investigated carbohydrates. Several carbon sources were tested as potential inducers for the α-galactosidase synthesis. On melibiose substrate α-galactosidase activity was higher in the intracellular fraction than in the filtrate of the fermentation broth, although both values were very low and did not reach the value of 1 U/mL. Raffinose, sucrose and Lactosucrose® proved to be inducers for α-galactosidase production. The highest titer (about 30 U/mL was achieved on 1 % sucrose and 0.45 % ammonium acetate. The optimum sucrose and ammonium acetate concentrations, at which about 90 U/mL α-galactosidase activity was reached during an 8-day fermentation, were 3 and 0.9 %, respectively.

  9. Novel progranulin mutation: screening for PGRN mutations in a Portuguese series of FTD/CBS cases.

    Science.gov (United States)

    Guerreiro, Rita Joao; Santana, Isabel; Bras, Jose Miguel; Revesz, Tamas; Rebelo, Olinda; Ribeiro, Maria Helena; Santiago, Beatriz; Oliveira, Catarina Resende; Singleton, Andrew; Hardy, John

    2008-07-15

    Mutations in the progranulin (PGRN) gene were recently described as the cause of ubiquitin positive frontotemporal dementia (FTD) in many families. Different frequencies of these genetic changes have been reported in diverse populations leading us to determine if these mutations were a major cause of FTD in the Portuguese population. The entire coding sequence plus exon 0 of PGRN were sequenced in a consecutive series of 46 FTD/CBS Portuguese patients. Two mutations were found: a novel pathogenic insertion (p.Gln300GlnfsX61) and a previously described point variant (p.T182M) of unclear pathogenicity. Pathogenic mutations in the PGRN gene were found in one of the 36 probands studied (3% of the probands in our series) who had a corticobasal syndrome presentation, indicating that in the Portuguese population, mutations in this gene are not a major cause of FTD.

  10. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H₂S pathway.

    Science.gov (United States)

    Wang, Z; Liu, D-X; Wang, F-W; Zhang, Q; Du, Z-X; Zhan, J-M; Yuan, Q-H; Ling, E-A; Hao, A-J

    2013-05-01

    Growing evidence has suggested that hydrogen sulfide (H₂S) acts as a novel neuro-modulator and neuroprotective agent; however, it remains to be investigated whether H2S has a direct effect on neural stem cells (NSCs). We report here that NSCs expressed cystathionine β synthase (CBS) and addition of exogenous H2S donor, L-cysteine, stimulated proliferation and increased the differentiation potential of NSCs to neurons and astroglia. Moreover, pre-treatment with aminooxyacetic acid, the inhibitor of CBS or knockdown of CBS in using siRNA, significantly attenuated the effects of L-cysteine on elevated H₂S levels and the cell proliferation; it also effectively suppressed L-cysteine-induced neurogenesis and astrocytogenesis. Further analysis revealed that L-cysteine-induced proliferation was associated with phosphorylation of extracellular signal-regulated kinases 1/2 and differentiation with altered expression of differentiation-related genes. Taken together, the present data suggest that L-cysteine can enhance proliferation and differentiation of NSCs via the CBS/H2S pathway, which may serve as a useful inference for elucidating its role in regulating the fate of NSCs in physiological and pathological settings. Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

  11. An assessment of selected properties of the fluorescent tracer, Tinopal CBS-X related to conservative behavior, and suggested improvements

    Science.gov (United States)

    Licha, Tobias; Niedbala, Anne; Bozau, Elke; Geyer, Tobias

    2013-03-01

    SummaryA conservative or well known reactive behavior of water tracers is a prerequisite in the quantitative evaluation of their tracer breakthrough curves. The fluorescent dye, Tinopal CBS-X, is one of the few licensed fluorescent dyes for water tracing with safe use. Its main advantage is its blue fluorescence, which is barely visible to the human eye and thus can be used when coloring water must be avoided. However, scientists have described the recovery of this dye as poor to very poor in field tracer experiments. Hence, this study focuses on examining the interaction of the main water chemistry with Tinopal CBS-X by determining the solubility products of the dye with most common cations. The findings of this investigation reveal that the tracer forms precipitates of very low aqueous solubility with di- and trivalent cations (pKsp 6.4-16.8). As a consequence, Tinopal CBS-X is not a conservative tracer and respective tracer breakthrough curves will exhibit strong tailings at least in part, as result of precipitations formed. The addition of a chemical modifier, EDTA, is suggested to enhance the solubility of Tinopal CBS-X in order to overcome its non-conservative behavior. Equations for estimating the amount of EDTA necessary are provided. In the light of these results, earlier reported tracer breakthrough curves are revisited and re-interpreted.

  12. Long-term performance of thermoplastic composite material with cotton burr and stem (CBS) as a partial filler

    Science.gov (United States)

    Rationale: Cotton burr and stem (CBS) fraction of cotton gin byproducts has shown promise as a fiber filler in thermoplastic composites, with physical and mechanical properties comparable to that made with wood fiber fillers. However, the long-term performance of this composite material is not known...

  13. Surface fatigue life and failure characteristics of EX-53, CBS 1000M, and AISI 9310 gear materials

    Science.gov (United States)

    Townsend, D. P.

    1985-01-01

    Spur gear endurance tests and rolling-element surface fatigue tests are conducted to investigate EX-53 and CBS 1000M steels for use as advanced application gear materials, to determine their endurance characteristics, and to compare the results with the standard AISI 9310 gear material. The gear pitch diameter is 8.89 cm (3.50 in). Gear test conditions are an oil inlet temperature of 320 K (116 F), an oil outlet temperature of 350 K (170 F), a maximum Hertz stress of 1.71 GPa (248 ksi), and a speed of 10,000 rpm. Bench-type rolling-element fatigue tests are conducted at ambient temperature with a bar specimen speed of 12,500 rpm and a maximum Hertz stress of 4.83 GPa (700 ksi). The EX-53 test gears have a surface fatigue life of twice that of the AISI 9310 spur gears. The CBS 1000M test gears have a surface fatigue life of more than twice that of the AISI 9310 spur gears. However, the CBS 1000M gears experience a 30-percent tooth fracture failure which limits its use as a gear material. The rolling-contact fatigue lines of RC bar specimens of EX-53 and ASISI 9310 are approximately equal. However, the CBS 1000M RC specimens have a surface fatigue life of about 50 percent that of the AISI 9310.

  14. Serotonin and dopamine protect from hypothermia/rewarming damage through the CBS/H2S pathway

    NARCIS (Netherlands)

    Talaei, Fatemeh; Bouma, Hjalmar; Van der Graaf, Adrianus; Strijkstra, Arjen; Schmidt, Martina; Henning, Robert

    2011-01-01

    Biogenic amines have been demonstrated to protect cells from apoptotic cell death. Herein we show for the first time that serotonin and dopamine increase H2S production by the endogenous enzyme cystathionine-beta-synthase (CBS) and protect cells against hypothermia/rewarming induced reactive oxygen

  15. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88

    DEFF Research Database (Denmark)

    Andersen, Mikael Rørdam; Salazar, Margarita Pena; Schaap, Peter J.

    2011-01-01

    in glucoamylase A, tRNA-synthases, and protein transporters in the protein producing CBS 513.88 strain. Our results and data sets from this integrative systems biology analysis resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi....

  16. Mutational analysis of residues in the regulatory CBS domains of Moorella thermoacetica pyrophosphatase corresponding to disease-related residues of human proteins.

    Science.gov (United States)

    Jämsen, Joonas; Tuominen, Heidi; Baykov, Alexander A; Lahti, Reijo

    2011-02-01

    mtCBS-PPase [CBS (cystathionine β-synthase) domain-containing pyrophosphatase from Moorella thermoacetica] contains a pair of CBS domains that strongly bind adenine nucleotides, thereby regulating enzyme activity. Eight residues associated with the CBS domains of mtCBS-PPase were screened to explore possible associations with regulation of enzyme activity. The majority of the substitutions (V99A, R168A, Y169A, Y169F, Y188A and H189A) enhanced the catalytic activity of mtCBS-PPase, two substitutions (R170A and R187G) decreased activity, and one substitution (K100G) had no effect. AMP-binding affinity was markedly decreased in the V99A, R168A and Y169A mutant proteins, and elevated in the R187G and H189A mutant proteins. Remarkably, the R168A and Y169A substitutions changed the effect of AMP from inhibition to activation. The stoichiometry of AMP binding increased from one to two AMP molecules per CBS domain pair in the Y169F, R170A, R187G and Y188A variants. The ADP-binding affinity decreased in three and increased in four mutant proteins. These findings identify residues determining the strength and selectivity of nucleotide binding, as well as the direction (inhibition or activation) of the subsequent effect. The data suggest that mutations in human CBS domain-containing proteins can be translated into a bacterial context. Furthermore, our data support the hypothesis that the CBS domains act as an 'internal inhibitor' of mtCBS-PPase.

  17. Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.

    Science.gov (United States)

    Zhang, Bi-li; Xu, Rong-liang; Zhang, Jing; Zhao, Xian-xian; Wu, Hong; Ma, Li-ping; Hu, Jian-qiang; Zhang, Jian-liang; Ye, Zhong; Zheng, Xing; Qin, Yong-wen

    2013-10-01

    PRKAG2 gene encodes the γ2 regulatory subunit of AMP-activated protein kinase (AMPK) that acts as a sensor of cellular energy status, and its germline mutations are responsible for PRKAG2 cardiac syndrome (PCS). The majority of missense mutations of cystathionine beta-synthase (CBS) domains found in PCS impair the binding activity of PRKAG2 to adenosine derivatives, and therefore lead to PRKAG2 function impairment and AMPK activity alteration, resulting in a familial syndrome of ventricular preexcitation, conduction defects, and cardiac hypertrophy. However, it is unclear about the PRKAG2 mutation in the non-CBS domain. Here, a Chinese family exhibiting the cardiac syndrome associated with a novel heterozygous PRKAG2 mutation (Gly100Ser) mapped to exon 3 encoding a non-CBS domain is described and the function of this novel mutation was investigated in vitro. The PRKAG2 G100S and R302Q mutations were constructed by a two-step polymerase chain reaction and then transfected into CCL13 cells by lentivirus vectors. Wild-type PRKAG2 gene transfection was used as a negative control. PRKAG2 expression was determined by Western blot. Immunofluorescence was used to localize the intracellular PRKAG2 proteins. MTT assay was performed to explore the effect of mutations on cell proliferation. Periodic acid-Schiff staining was used for detecting glycogen accumulation. AMPK concentration was measured with enzyme-linked immunosorbent assay. Our results showed neither intracellular localization of PRKAG2 nor cell growth was altered. In contrast, PRKAG2 protein expression levels were significantly reduced by this mutation. Furthermore, PRKAG2-mediated activity of AMPK was attenuated, resulting in glycogen metabolism dysregulation. These findings revealed that non-CBS domains of PRKAG2 were essential to the regulation of AMPK activity, similar to CBS. Our study ascribes a crucial regulatory role to the novel PRKAG2 G100S mutation, and reiterates that PCS occurs as a consequence of

  18. Detailed kinetic study of the ring opening of cycloalkanes by CBS-QB3 calculations.

    Science.gov (United States)

    Sirjean, B; Glaude, P A; Ruiz-Lopez, M F; Fournet, R

    2006-11-23

    This work reports a theoretical study of the gas-phase unimolecular decomposition of cyclobutane, cyclopentane and cyclohexane by means of quantum chemical calculations. A biradical mechanism has been envisaged for each cycloalkane, and the main routes for the decomposition of the biradicals formed have been investigated at the CBS-QB3 level of theory. Thermochemical data(DeltaHf(o), S(o), Cp(o)) for all the involved species have been obtained by means of isodesmic reactions. The contribution of hindered rotors has also been included. Activation barriers of each reaction have been analyzed to assess the energetically most favorable pathways for the decomposition of biradicals. Rate constants have been derived for all elementary reactions using transition-state theory at 1 atm and temperatures ranging from 600 to 2000 K. Global rate constant for the decomposition of the cyclic alkanes in molecular products have been calculated. Comparison between calculated and experimental results allowed us to validate the theoretical approach. An important result is that the rotational barriers between the conformers, which are usually neglected, are of importance in decomposition rate of the largest biradicals. Ring strain energies (RSE) in transition states for ring opening have been estimated and show that the main part of RSE contained in the cyclic reactants is removed upon the activation process.

  19. Investigation of CBS, MTR, RFC-1 and TC polymorphisms as maternal risk factors for Down syndrome.

    Science.gov (United States)

    Fintelman-Rodrigues, N; Corrêa, J C; Santos, J M; Pimentel, M M G; Santos-Rebouças, C B

    2009-01-01

    Recent evidence shows that almost 92% of the DS children are born from young mothers, suggesting that other risk factors than advanced maternal age must be involved. In this context, some studies demonstrated a possible link between DS and maternal polymorphisms in genes involved in folate metabolism. These polymorphisms, as well as low intake of folate could generate genomic instability, DNA hypomethylation and abnormal segregation, leading to trisomy 21. We compared the frequency of CBS 844ins68, MTR 2756A>G, RFC-1 80G> A and TC 776C>G polymorphisms among 114 case mothers and 110 matched controls, in order to observe whether these variants act as risk factors for DS. The genotype distributions revealed that there were not significant differences between both samples. However, when we proceed the multiplicative interaction analyses between the four polymorphisms described above together with the previously studied MTHFR 677C>T, MTHFR 1298A>C and MTRR 66A>G polymorphisms, our results show that the combined genotype TC 776CC / MTHFR 677TT and TC 776CC / MTR 2756AG were significantly higher in the control sample. Nevertheless, there was no significant association after Bonferroni correction. Our results suggest that maternal folate-related polymorphisms studied here have no influence on trisomy 21 susceptibility in subjects of Brazilian population.

  20. Three New Butenolides from the Fungus Aspergillus sp. CBS-P-2

    Directory of Open Access Journals (Sweden)

    Xiao An

    2016-10-01

    Full Text Available Three new butenolides aspernolides H–J (1–3 together with seven known ones (4–10 were isolated from the fungus Aspergillus sp. CBS-P-2. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopic data, HR-ESI-MS analysis, and their absolute configuration were determined by circular dichroism (CD analysis. All the compounds were evaluated for the antioxidant effects by DPPH and ABTS methods, the antitumor activities against four human tumor cell lines (HL-60, ASPC1, HCT-116 and PC-3 and antimicrobial activities. Compounds 4–10 showed significant activity against DPPH (IC50 = 15.9–34.3 μM and compounds 1–10 exhibited significant ABTS free radical scavenging activity (IC50 = 2.8–33.1 μM. Compounds 2, 5 and 11 showed potent cytotoxic activities against HL-60 cell lines with IC50 values of 39.4, 13.2 and 16.3 μM, respectively. Compound 10 showed good antimicrobial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC of 21.3 μM.

  1. Development of AN Innovative Three-Dimensional Complete Body Screening Device - 3D-CBS

    Science.gov (United States)

    Crosetto, D. B.

    2004-07-01

    This article describes an innovative technological approach that increases the efficiency with which a large number of particles (photons) can be detected and analyzed. The three-dimensional complete body screening (3D-CBS) combines the functional imaging capability of the Positron Emission Tomography (PET) with those of the anatomical imaging capability of Computed Tomography (CT). The novel techniques provide better images in a shorter time with less radiation to the patient. A primary means of accomplishing this is the use of a larger solid angle, but this requires a new electronic technique capable of handling the increased data rate. This technique, combined with an improved and simplified detector assembly, enables executing complex real-time algorithms and allows more efficiently use of economical crystals. These are the principal features of this invention. A good synergy of advanced techniques in particle detection, together with technological progress in industry (latest FPGA technology) and simple, but cost-effective ideas provide a revolutionary invention. This technology enables over 400 times PET efficiency improvement at once compared to two to three times improvements achieved every five years during the past decades. Details of the electronics are provided, including an IBM PC board with a parallel-processing architecture implemented in FPGA, enabling the execution of a programmable complex real-time algorithm for best detection of photons.

  2. Utilisation of aromatic organosulfur compounds as sulfur sources by Lipomyces starkeyi CBS 1807.

    Science.gov (United States)

    Linder, Tomas

    2016-10-01

    Aromatic organosulfur compounds that contain aryl carbon-sulfur bonds are rare in nature but occur frequently in industrial processes and products. The utilisation of aryl sulfur compounds as a sulfur source has not been described previously among the budding yeasts (phylum Ascomycota, sub-phylum Saccharomycotina). A total of 31 strains representing 30 species and 28 genera of the budding yeasts were assayed for utilisation of benzene sulfonate as source of sulfur. Lipomyces starkeyi CBS 1807 was the only strain in this screen that was able to utilise benzene sulfonate as a sulfur source. Further study of L. starkeyi demonstrated that it could also utilise methyl phenyl sulfoxide, 1-naphthalene sulfonate, 2-naphthalene sulfonate, 1-anthraquinone sulfonate and 2-anthraquinone sulfonate as sulfur sources but not diphenylsulfone. This study represents the first reported case of the utilisation of sulfonated naphthalenes and anthraquinones as sulfur sources by a fungus. However, it should be stressed that the remaining 30 strains in this study were not assayed for the utilisation of these additional aryl sulfur compounds as sulfur sources. The present study highlights the potential of L. starkeyi and related species as a source of novel enzyme activities with possible applications in chemical manufacturing, disposal of xenobiotics and bioremediation.

  3. PSP-CBS with Dopamine Deficiency in a Female with a FMR1 Premutation.

    Science.gov (United States)

    Paucar, Martin; Beniaminov, Stanislav; Paslawski, Wojciech; Svenningsson, Per

    2016-10-01

    Premutations in the fragile X mental retardation 1 (FMR1) gene cause fragile X-associated tremor/ataxia syndrome (FXTAS) and FMR1-related primary ovarian insufficiency (POI). Female FMR1 premutation carriers rarely develop motor features. Dual pathology is an emerging phenomenon among FMR1 premutation carriers. Here, we describe a family affected by FMR1-related disorders in which the female index case has developed a rapidly progressive and disabling syndrome of atypical parkinsonism. This syndrome consists of early onset postural instability, echolalia, dystonia, and varying types of apraxia like early onset orobuccal apraxia and oculomotor apraxia. She has also developed supranuclear gaze palsy, increased latency of saccade initiation, and slow saccades. These features are compatible with progressive supranuclear palsy (PSP) of a corticobasal syndrome (CBS) variant. Imaging displays a marked reduction of presynaptic dopaminergic uptake and cerebrospinal fluid analysis showed reduced dopamine metabolism; however, the patient is unresponsive to levodopa. Midbrain atrophy ("hummingbird sign") and mild cerebellar atrophy were found on brain MRI. Her father was affected by a typical FXTAS presentation but also displayed dopamine deficiency along with the hummingbird sign. The mechanisms by which FMR1 premutations predispose to atypical parkinsonism and dopamine deficiency await further elucidation.

  4. Three New Butenolides from the Fungus Aspergillus sp. CBS-P-2.

    Science.gov (United States)

    An, Xiao; Pei, Yuehu; Chen, Shaofei; Li, Shengge; Hu, Xiaolong; Chen, Gang; Lin, Bin; Wang, Haifeng

    2016-10-13

    Three new butenolides aspernolides H-J (1-3) together with seven known ones (4-10) were isolated from the fungus Aspergillus sp. CBS-P-2. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopic data, HR-ESI-MS analysis, and their absolute configuration were determined by circular dichroism (CD) analysis. All the compounds were evaluated for the antioxidant effects by DPPH and ABTS methods, the antitumor activities against four human tumor cell lines (HL-60, ASPC1, HCT-116 and PC-3) and antimicrobial activities. Compounds 4-10 showed significant activity against DPPH (IC50 = 15.9-34.3 μM) and compounds 1-10 exhibited significant ABTS free radical scavenging activity (IC50 = 2.8-33.1 μM). Compounds 2, 5 and 11 showed potent cytotoxic activities against HL-60 cell lines with IC50 values of 39.4, 13.2 and 16.3 μM, respectively. Compound 10 showed good antimicrobial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 21.3 μM.

  5. Production, Distribution, and Kinetic Properties of Inulinase in Continuous Cultures of Kluyveromyces marxianus CBS 6556

    Science.gov (United States)

    Rouwenhorst, Robert J.; Visser, Leo E.; Van Der Baan, Adriaan A.; Scheffers, W. Alexander; Van Dijken, Johannes P.

    1988-01-01

    From a screening of several Kluyveromyces strains, the yeast Kluyveromyces marxianus CBS 6556 was selected for a study of the parameters relevant to the commercial production of inulinase (EC 3.2.1.7). This yeast exhibited superior properties with respect to growth at elevated temperatures (40 to 45°C), substrate specificity, and inulinase production. In sucrose-limited chemostat cultures growing on mineral medium, the amount of enzyme decreased from 52 U mg of cell dry weight−1 at D = 0.1 h−1 to 2 U mg of cell dry weight−1 at D = 0.8 h−1. Experiments with nitrogen-limited cultures further confirmed that synthesis of the enzyme is negatively controlled by the residual sugar concentration in the culture. High enzyme activities were observed during growth on nonsugar substrates, indicating that synthesis of the enzyme is a result of a derepression/repression mechanism. A substantial part of the inulinase produced by K. marxianus was associated with the cell wall. The enzyme could be released from the cell wall via a simple chemical treatment of cells. Results are presented on the effect of cultivation conditions on the distribution of the enzyme. Inulinase was active with sucrose, raffinose, stachyose, and inulin as substrates and exhibited an S/I ratio (relative activities with sucrose and inulin) of 15 under standard assay conditions. The enzyme activity decreased with increasing chain length of the substrate. PMID:16347626

  6. Investigation of CBS, MTR, RFC-1 and TC Polymorphisms as Maternal Risk Factors for Down Syndrome

    Directory of Open Access Journals (Sweden)

    N. Fintelman-Rodrigues

    2009-01-01

    Full Text Available Recent evidence shows that almost 92% of the DS children are born from young mothers, suggesting that other risk factors than advanced maternal age must be involved. In this context, some studies demonstrated a possible link between DS and maternal polymorphisms in genes involved in folate metabolism. These polymorphisms, as well as low intake of folate could generate genomic instability, DNA hypomethylation and abnormal segregation, leading to trisomy 21. We compared the frequency of CBS 844ins68, MTR 2756A>G, RFC-1 80G> A and TC 776C>G polymorphisms among 114 case mothers and 110 matched controls, in order to observe whether these variants act as risk factors for DS. The genotype distributions revealed that there were not significant differences between both samples. However, when we proceed the multiplicative interaction analyses between the four polymorphisms described above together with the previously studied MTHFR 677C>T, MTHFR 1298A>C and MTRR 66A>G polymorphisms, our results show that the combined genotype TC 776CC / MTHFR 677TT and TC 776CC / MTR 2756AG were significantly higher in the control sample. Nevertheless, there was no significant association after Bonferroni correction. Our results suggest that maternal folate-related polymorphisms studied here have no influence on trisomy 21 susceptibility in subjects of Brazilian population.

  7. Investigation of CBS, MTR, RFC-1 and TC Polymorphisms as Maternal Risk Factors for Down Syndrome

    Science.gov (United States)

    Fintelman-Rodrigues, N.; Corrêa, J. C.; Santos, J. M.; Pimentel, M. M. G.; Santos-Rebouças, C. B.

    2009-01-01

    Recent evidence shows that almost 92% of the DS children are born from young mothers, suggesting that other risk factors than advanced maternal age must be involved. In this context, some studies demonstrated a possible link between DS and maternal polymorphisms in genes involved in folate metabolism. These polymorphisms, as well as low intake of folate could generate genomic instability, DNA hypomethylation and abnormal segregation, leading to trisomy 21. We compared the frequency of CBS 844ins68, MTR 2756A>G, RFC-1 80G> A and TC 776C>G polymorphisms among 114 case mothers and 110 matched controls, in order to observe whether these variants act as risk factors for DS. The genotype distributions revealed that there were not significant differences between both samples. However, when we proceed the multiplicative interaction analyses between the four polymorphisms described above together with the previously studied MTHFR 677C>T, MTHFR 1298A>C and MTRR 66A>G polymorphisms, our results show that the combined genotype TC 776CC / MTHFR 677TT and TC 776CC / MTR 2756AG were significantly higher in the control sample. Nevertheless, there was no significant association after Bonferroni correction. Our results suggest that maternal folate-related polymorphisms studied here have no influence on trisomy 21 susceptibility in subjects of Brazilian population. PMID:19729796

  8. Analysing the Suzaku spectra of the broad-line Seyfert 1 galaxy CBS 126

    Science.gov (United States)

    Chiang, Chia-Ying; Reis, R. C.; Fabian, A. C.; Grupe, D.; Tsuruta, S.

    2012-09-01

    We analysed new simultaneous Suzaku and Swift data of the broad-line Seyfert 1 (BLS1) galaxy CBS 126. A clear Fe emission line and a strong soft excess are present in the source spectra. We fit the spectra with a relativistic reflection model and find that the model tends to fit the data with a high iron abundance possibly due to the large soft excess present. By checking the difference and the rms spectra, we find that there is likely an absorption edge at ˜0.89 keV, which might be caused by oxygen or neon. We also produced an analysis of the time-resolved spectra in order to examine the existence of the edge. Although high iron abundance is required more in the time-resolved spectra, a model of solar iron abundance together with an absorption edge gives a more physical explanation. Variation of the ionization parameter is an alternative, plausible explanation for the excess seen in the difference spectra. It is difficult to know if there are warm absorbers in this source from the current data. To further investigate the presence of possible warm absorbers, higher signal-to-noise ratio low-energy data are needed. The work presented here tentatively suggests that the spectra of a BLS1 galaxy can be explained by a relativistic reflection model similar to that often seen in their narrow-line cousins.

  9. Chaetochromones A and B, Two New Polyketides from the Fungus Chaetomium indicum (CBS.860.68

    Directory of Open Access Journals (Sweden)

    Xuewei Wang

    2013-09-01

    Full Text Available Chaetochromones A (1 and B (2, two novel polyketides, were isolated from the crude extract of fungus Chaetomium indicum (CBS.860.68 together with three known analogues PI-3(3, PI-4 (4 and SB236050 (5. The structures of these compounds were determined by HRESI-MS and NMR experiments. Chaetochromones A (1 and B (2 are a member of the polyketides family, which might originate from a similar biogenetic pathway as the known compounds PI-3 (3, PI-4 (4 and SB236050 (5. The biological activities of these secondary metabolites were evaluated against eight plant pathogens, including Alternaria alternata, Ilyonectria radicicola, Trichoderma viride pers, Aspergillus niger, Fusarium verticillioide, Irpex lacteus (Fr., Poria placenta (Fr. Cooke and Coriolus versicolor (L. Quél. Compound 1 displayed moderate inhibitory rate (>60% against the brown rot fungus Poria placenta (Fr. Cooke, which causes significant wood decay. In addition, the cytotoxic activities against three cancer cell lines A549, MDA-MB-231, PANC-1 were also tested, without any inhibitory activities being detected.

  10. Cloning and Characterization of Two Pyruvate Decarboxylase Genes from Pichia stipitis CBS 6054

    Science.gov (United States)

    Lu, Ping; Davis, Brian P.; Jeffries, Thomas W.

    1998-01-01

    In Pichia stipitis, fermentative and pyruvate decarboxylase (PDC) activities increase with diminished oxygen rather than in response to fermentable sugars. To better characterize PDC expression and regulation, two genes for PDC (PsPDC1 and PsPDC2) were cloned and sequenced from P. stipitis CBS 6054. Aside from Saccharomyces cerevisiae, from which three PDC genes have been characterized, P. stipitis is the only organism from which multiple genes for PDC have been identified and characterized. PsPDC1 and PsPDC2 have diverged almost as far from one another as they have from the next most closely related known yeast gene. PsPDC1 contains an open reading frame of 1,791 nucleotides encoding 597 amino acids. PsPDC2 contains a reading frame of 1,710 nucleotides encoding 570 amino acids. An 81-nucleotide segment in the middle of the β domain of PsPDC1 codes for a unique segment of 27 amino acids, which may play a role in allosteric regulation. The 5′ regions of both P. stipitis genes include two putative TATA elements that make them similar to the PDC genes from S. cerevisiae, Kluyveromyces marxianus, and Hanseniaspora uvarum. PMID:9435065

  11. Ethanol production using whole plant biomass of Jerusalem artichoke by Kluyveromyces marxianus CBS1555.

    Science.gov (United States)

    Kim, Seonghun; Park, Jang Min; Kim, Chul Ho

    2013-03-01

    Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.

  12. Assessment of Performance of G3B3 and CBS-QB3 Methods in Calculation of Bond Dissociation Energies

    Institute of Scientific and Technical Information of China (English)

    亓秀娟; 封勇; 刘磊; 郭庆祥

    2005-01-01

    The performance of the newly developed G3B3 and CBS-QB3 methods in calculating absolute bond dissociation energy (BDE) was assessed. It was found that these two methods could predict the BDE with an accuracy of about 8.4 kJ/mol and therefore, they exlfibited similar performance as the standard G3 and CBS-Q methods. On the other hand, it was demonstrated that the B3LYP method significantly underestimated the absolute BDE by 16.7—20.9 kJ/mol. This finding was valuable and timely because many researchers could use this relatively cheap method in studying radical reactions. Finally, 38 compounds were showed for which the theoretical BDE seriously deviated from the experimental data.

  13. Stacking of benzene with metal chelates: calculated CCSD(T)/CBS interaction energies and potential-energy curves.

    Science.gov (United States)

    Malenov, Dušan P; Ninković, Dragan B; Sredojević, Dušan N; Zarić, Snežana D

    2014-08-25

    Accurate values for the energies of stacking interactions of nickel- and copper-based six-membered chelate rings with benzene are calculated at the CCSD(T)/CBS level. The results show that calculations made at the ωB97xD/def2-TZVP level are in excellent agreement with CCSD(T)/CBS values. The energies of [Cu(C3H3O2)(HCO2)] and [Ni(C3H3O2)(HCO2)] chelates stacking with benzene are -6.39 and -4.77 kcal mol(-1), respectively. Understanding these interactions might be important for materials with properties that are dependent on stacking interactions. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. A scandal on screen : a gendered reading of Arthur Conan Doyle's characters in BBC's 'Sherlock' and CBS's 'Elementary'

    OpenAIRE

    2014-01-01

    The object of this paper is to denaturalise and deconstruct the way in which gender is written and represented in popular television. To do so, I will examine two recent television adaptations of Sir Arthur Conan Doyle's Sherlock Holmes detective stories: BBC's Sherlock (2010–) and CBS's Elementary (2012–). This choice responds to the fact that although both series are based on the same original text, each takes a completely different position in terms of gender representation. I will argue t...

  15. Cognitive impairment in ALS patients and validation of the Spanish version of the ALS-CBS test.

    Science.gov (United States)

    Turon-Sans, Janina; Gascon-Bayarri, Jordi; Reñé, Ramon; Rico, Imma; Gámez, Cristina; Paipa, Andres; Povedano, Monica

    2016-01-01

    Our aim was to develop and validate the Spanish version of the Amyotrophic Lateral Sclerosis Cognitive Behavioural Screen (ALS-CBS) and investigate behavioural/cognitive impairment in our ALS patients. We enrolled 50 patients with definite or probable ALS, evaluated by the Motor Neuron Disease Unit (using El Escorial criteria) and Dementia Unit, and assessed with the Spanish ALS-CBS. The patients' cognitive/behavioural status was classified according to current criteria. Patients were classified into each diagnostic category: ALS-no impairment, 36%; ALS-mild cognitive impairment, 34%; ALS-mild behavioural impairment, 6%; ALS-mild cognitive/behavioural impairment, 12%; ALS-frontotemporal dementia, 12%. Cognitive impairment was more common in bulbar (90.9%) than spinal (48.7%) forms (p CBS was validated. Performance to differentiate normal vs. impaired individuals was: 1) cognition (cut-off 15; AUC, 84.7%): sensitivity 86.2%, specificity 62%, positive predictive value 75.8%, negative predictive value 76.5%; 2) behaviour (cut-off 36; AUC, 83.3%): sensitivity 93.3%, specificity 74.3%, positive predictive value 61%, negative predictive value 96.3%. Performance to differentiate between patients with and without dementia: 1) cognition (cut-off 8; AUC, 87.3%): sensitivity 83.3%, specificity 75%, positive predictive value 31.3%, negative predictive value 97.1%; 2) behaviour (cut-off 35; AUC, 80.9%): sensitivity 83.3%, specificity 69%, positive predictive value 25%, negative predictive value 96.7%. In conclusion, cognitive impairment is common in ALS patients, particularly in bulbar forms. The Spanish version of the ALS-CBS is useful for screening cognitive/behavioural impairment in this population.

  16. A missense mutation in the cystathionine {beta}-synthase (CBS) gene associated with pyridoxine (B{sub 6}) responsive homocystinuria

    Energy Technology Data Exchange (ETDEWEB)

    Shih, V.E.; Fringer, J.M.; Mandell, R. [Massachusetts General Hospital, Boston, MA (United States)] [and others

    1994-09-01

    CBS deficiency is an autosomal recessive disorder characterized by homocystinuria and multisystem clinical disease. B{sub 6} responsive patients usually have a milder clinical phenotype than B{sub 6} nonresponsive patients. In our ongoing studies of the molecular defects in CBS deficiency, we reported a T-833 to C transition causing a substitution of threonine for isoleucine at position 278 (I278T). By PCR amplification and sequencing of exon 8 from genomic DNA we have now identified 2 index patients who are homozygous and 5 who are heterozygous for this mutation in a group of 32 patients with CBS deficiency. The mutation was detected in 7 of 10 unrelated families with in vivo B{sub 6} responsiveness, including one with a partial response, and in 0 of 22 B{sub 6} nonresponsive patients. The mutations on the other allele in the compound heterozygotes are still under investigation. We have now observed the I278T mutation in 9 of 20 independent alleles of varied ethnic backgrounds in the subgroup of B{sub 6} responsive patients. These findings, together with the previous report of this mutation in one allele of a B{sub 6} responsive patient, suggest that the I278T mutation is associated with B{sub 6} responsiveness in CBS deficiency. In compound heterozygotes, the degree of B{sub 6} responsiveness may also depend upon the nature of the other mutant allele and/or the interaction between the polypeptide subunits produced by the two mutant allelic genes.

  17. Transcriptome of the Alternative Ethanol Production Strain Dekkera bruxellensis CBS 11270 in Sugar Limited, Low Oxygen Cultivation

    OpenAIRE

    Ievgeniia A Tiukova; Petterson, Mats E.; Christian Tellgren-Roth; Ignas Bunikis; Thomas Eberhard; Olga Vinnere Pettersson; Volkmar Passoth

    2013-01-01

    Dekkera bruxellensis can outcompete Saccharomyces cerevisiae in environments with low sugar concentrations. It is usually regarded as a spoilage yeast but has lately been identified as an alternative ethanol production organism. In this study, global gene expression in the industrial isolate D. bruxellensis CBS 11270 under oxygen and glucose limitation was investigated by whole transcriptome sequencing using the AB SOLiD technology. Among other observations, we noted expression of respiratory...

  18. Hartree-Fock and density functional complete basis set (CBS) predicted shielding anisotropy and shielding tensor components.

    Energy Technology Data Exchange (ETDEWEB)

    Kupka, T.; Ruscic, B.; Botto, R. E.; Chemistry

    2003-05-01

    The nuclear shielding anisotropy and shielding tensor components calculated using the hybrid density functional B3PW91 are reported for a model set of compounds comprised of N{sub 2}, NH{sub 3}, CH{sub 4}, C{sub 2}H{sub 4}, HCN and CH{sub 3}CN. An estimation of density functional theory (DFT) and Hartree-Fock complete basis-set limit (CBS) parameters from a 2 (3) point exact fit vs. least-squares fit was obtained with the cc-pVxZ and aug-cc-pVxZ basis sets (x=D, T, Q, 5, 6). Both Hartree-Fock- and DFT-predicted CBS shielding anisotropies and shielding tensor components of the model molecules were in reasonable agreement with available experimental data. The utility of using a limited CBS approach for calculating accurate anisotropic shielding parameters of larger molecules as complementary methods to solid-state NMR is proposed.

  19. CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations

    Science.gov (United States)

    Scott, John W.; Hawley, Simon A.; Green, Kevin A.; Anis, Miliea; Stewart, Greg; Scullion, Gillian A.; Norman, David G.; Hardie, D. Grahame

    2004-01-01

    CBS domains are defined as sequence motifs that occur in several different proteins in all kingdoms of life. Although thought to be regulatory, their exact functions have been unknown. However, their importance was underlined by findings that mutations in conserved residues within them cause a variety of human hereditary diseases, including (with the gene mutated in parentheses): Wolff-Parkinson-White syndrome (γ2 subunit of AMP-activated protein kinase); retinitis pigmentosa (IMP dehydrogenase-1); congenital myotonia, idiopathic generalized epilepsy, hypercalciuric nephrolithiasis, and classic Bartter syndrome (CLC chloride channel family members); and homocystinuria (cystathionine β-synthase). AMP-activated protein kinase is a sensor of cellular energy status that is activated by AMP and inhibited by ATP, but the location of the regulatory nucleotide-binding sites (which are prime targets for drugs to treat obesity and diabetes) was not characterized. We now show that tandem pairs of CBS domains from AMP-activated protein kinase, IMP dehydrogenase-2, the chloride channel CLC2, and cystathionine β-synthase bind AMP, ATP, or S-adenosyl methionine,while mutations that cause hereditary diseases impair this binding. This shows that tandem pairs of CBS domains act, in most cases, as sensors of cellular energy status and, as such, represent a newly identified class of binding domain for adenosine derivatives. PMID:14722619

  20. Structural basis for substrate activation and regulation by cystathionine beta-synthase (CBS) domains in cystathionine [beta]-synthase

    Energy Technology Data Exchange (ETDEWEB)

    Koutmos, Markos; Kabil, Omer; Smith, Janet L.; Banerjee, Ruma (Michigan-Med)

    2011-08-17

    The catalytic potential for H{sub 2}S biogenesis and homocysteine clearance converge at the active site of cystathionine {beta}-synthase (CBS), a pyridoxal phosphate-dependent enzyme. CBS catalyzes {beta}-replacement reactions of either serine or cysteine by homocysteine to give cystathionine and water or H{sub 2}S, respectively. In this study, high-resolution structures of the full-length enzyme from Drosophila in which a carbanion (1.70 {angstrom}) and an aminoacrylate intermediate (1.55 {angstrom}) have been captured are reported. Electrostatic stabilization of the zwitterionic carbanion intermediate is afforded by the close positioning of an active site lysine residue that is initially used for Schiff base formation in the internal aldimine and later as a general base. Additional stabilizing interactions between active site residues and the catalytic intermediates are observed. Furthermore, the structure of the regulatory 'energy-sensing' CBS domains, named after this protein, suggests a mechanism for allosteric activation by S-adenosylmethionine.

  1. CBS: an open platform that integrates predictive methods and epigenetics information to characterize conserved regulatory features in multiple Drosophila genomes

    Directory of Open Access Journals (Sweden)

    Blanco Enrique

    2012-12-01

    Full Text Available Abstract Background Information about the composition of regulatory regions is of great value for designing experiments to functionally characterize gene expression. The multiplicity of available applications to predict transcription factor binding sites in a particular locus contrasts with the substantial computational expertise that is demanded to manipulate them, which may constitute a potential barrier for the experimental community. Results CBS (Conserved regulatory Binding Sites, http://compfly.bio.ub.es/CBS is a public platform of evolutionarily conserved binding sites and enhancers predicted in multiple Drosophila genomes that is furnished with published chromatin signatures associated to transcriptionally active regions and other experimental sources of information. The rapid access to this novel body of knowledge through a user-friendly web interface enables non-expert users to identify the binding sequences available for any particular gene, transcription factor, or genome region. Conclusions The CBS platform is a powerful resource that provides tools for data mining individual sequences and groups of co-expressed genes with epigenomics information to conduct regulatory screenings in Drosophila.

  2. Unique gating properties of C. elegans ClC anion channel splice variants are determined by altered CBS domain conformation and the R-helix linker.

    Science.gov (United States)

    Dave, Sonya; Sheehan, Jonathan H; Meiler, Jens; Strange, Kevin

    2010-01-01

    All eukaryotic and some prokaryotic ClC anion transport proteins have extensive cytoplasmic C-termini containing two cystathionine-β-synthase (CBS) domains. CBS domain secondary structure is highly conserved and consists of two α-helices and three β-strands arranged as β1-α1-β2-β3-α2. ClC CBS domain mutations cause muscle and bone disease and alter ClC gating. However, the precise functional roles of CBS domains and the structural bases by which they regulate ClC function are poorly understood. CLH-3a and CLH-3b are C. elegans ClC anion channel splice variants with strikingly different biophysical properties. Splice variation occurs at cytoplasmic N- and C-termini and includes several amino acids that form α2 of the second CBS domain (CBS2). We demonstrate that interchanging α2 between CLH-3a and CLH-3b interchanges their gating properties. The "R-helix" of ClC proteins forms part of the ion-conducting pore and selectivity filter and is connected to the cytoplasmic C-terminus via a short stretch of cytoplasmic amino acids termed the "R-helix linker". C-terminus conformation changes could cause R-helix structural rearrangements via this linker. X-ray structures of three ClC protein cytoplasmic C-termini suggest that α2 of CBS2 and the R-helix linker could be closely apposed and may therefore interact. We found that mutating apposing amino acids in α2 and the R-helix linker of CLH-3b was sufficient to give rise to CLH-3a-like gating. We postulate that the R-helix linker interacts with CBS2 α2, and that this putative interaction provides a pathway by which cytoplasmic C-terminus conformational changes induce conformational changes in membrane domains that in turn modulate ClC function.

  3. A novel transgenic mouse model of CBS-deficient homocystinuria does not incur hepatic steatosis or fibrosis and exhibits a hypercoagulative phenotype that is ameliorated by betaine treatment.

    Science.gov (United States)

    Maclean, Kenneth N; Sikora, Jakub; Kožich, Viktor; Jiang, Hua; Greiner, Lori S; Kraus, Eva; Krijt, Jakub; Overdier, Katherine H; Collard, Renata; Brodsky, Gary L; Meltesen, Lynne; Crnic, Linda S; Allen, Robert H; Stabler, Sally P; Elleder, Milan; Rozen, Rima; Patterson, David; Kraus, Jan P

    2010-01-01

    Cystathionine beta-synthase (CBS) catalyzes the condensation of homocysteine (Hcy) and serine to cystathionine, which is then hydrolyzed to cysteine by cystathionine gamma-lyase. Inactivation of CBS results in CBS-deficient homocystinuria more commonly referred to as classical homocystinuria, which, if untreated, results in mental retardation, thromboembolic complications, and a range of connective tissue disorders. The molecular mechanisms that underlie the pathology of this disease are poorly understood. We report here the generation of a new mouse model of classical homocystinuria in which the mouse cbs gene is inactivated and that exhibits low-level expression of the human CBS transgene under the control of the human CBS promoter. This mouse model, designated "human only" (HO), exhibits severe elevations in both plasma and tissue levels of Hcy, methionine, S-adenosylmethionine, and S-adenosylhomocysteine and a concomitant decrease in plasma and hepatic levels of cysteine. HO mice exhibit mild hepatopathy but, in contrast to previous models of classical homocystinuria, do not incur hepatic steatosis, fibrosis, or neonatal death with approximately 90% of HO mice living for at least 6months. Tail bleeding determinations indicate that HO mice are in a hypercoagulative state that is significantly ameliorated by betaine treatment in a manner that recapitulates the disease as it occurs in humans. Our findings indicate that this mouse model will be a valuable tool in the study of pathogenesis in classical homocystinuria and the rational design of novel treatments. Copyright © 2010 Elsevier Inc. All rights reserved.

  4. Identification of Lipstatin-Producing Ability in Streptomyces virginiae CBS 314.55 Using Dereplication Approach

    Directory of Open Access Journals (Sweden)

    Gordan Sladič

    2014-01-01

    Full Text Available Streptomyces species are prolific producers of bioactive metabolites, such as β-lactone-containing lipstatin produced by Streptomyces toxytricini, an intermediate used in semi-synthetic process for production of anti-obesity drug orlistat. Understanding the distribution of identical or structurally similar molecules produced by a taxonomic group is of particular importance when trying to isolate novel biologically active compounds or strains producing known metabolites of medical importance with potentially improved properties. Until now, only two independent isolates of S. toxytricini species have been known to be producers of lipstatin. According to the current taxonomic criteria, S. toxytricini belongs to Streptomyces lavendulae phenotypic cluster. Taxonomy-based dereplication approach coupled with in vitro assay was applied to screen the S. lavendulae phenotypic cluster for production of lipstatin-like lipase inhibitors using synthetic p-nitrophenol derivatives of C4 and C16 lipids. Screening the available strains from public collections belonging to S. lavendulae phenotypic cluster, high lipase inhibitory activity was identified in the Streptomyces virginiae CBS 314.55 culture supernatants. HPLC and LC-MS/MS confirmed lipstatin production by a new Streptomyces species for the first time. We have demonstrated that the new lipstatin-producing strain S. virginiae morphologically and physiologically differs from S. toxytricini substantially; however, the production capacity of the newly identified lipstatin-producing species S. virginiae is comparable to S. toxytricini. We have thus demonstrated the effectiveness of a simple and affordable dereplication approach for identification of potentially novel and useful industrial strains available in public culture collections.

  5. Impaired CBS-H2S signaling axis contributes to MPTP-induced neurodegeneration in a mouse model of Parkinson's disease.

    Science.gov (United States)

    Yuan, Yu-Qing; Wang, Ya-Li; Yuan, Bao-Shi; Yuan, Xin; Hou, Xiao-Ou; Bian, Jin-Song; Liu, Chun-Feng; Hu, Li-Fang

    2017-08-01

    Hydrogen sulfide (H2S), a novel neuromodulator, is linked to the pathogenesis of several neurodegenerative disorders. Exogenous application of H2S exerts neuroprotection via anti-inflammation and anti-oxidative stress in animal and cellular models of Parkinson's disease (PD). However, the role of endogenous H2S and the contribution of its various synthases in PD remain unclear. In the present study, we found a decline of plasma and striatal sulfide level in 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced PD mouse model. Interestingly, among the three H2S generating enzymes, only cystathionine β-synthase (CBS) expression was largely reduced in the striatum of MPTP-treated mice. The in vitro study confirmed a significant decrease of CBS expression in 1-methyl-4-phenylpyridinium (MPP(+))-stimulated astrocytes and microglia, but not in neurons or SH-SY5Y dopaminergic cells. Striatal CBS overexpression, elicited by stereotaxic delivery with Cbs gene using recombinant adeno-associated-virus (rAAV-Cbs), successfully enhanced the sulfide level in the striatum and partially rescued the MPTP-induced dopaminergic neurotoxicity in the midbrain. Specifically, striatal CBS overexpression alleviated the motor deficits and dopaminergic neuron losses in the nigro-striatal pathway, with a concomitant inhibition of glial activation in MPTP-treated mice. Furthermore, compared to rAAV-Vector, rAAV-Cbs injection reduced the aberrant accumulation of nitric oxide and 3-nitrotyrosine (an indicator of protein nitration) in the striatum of MPTP-treated mice. Notably, it also attenuated the increase of nitrated α-synuclein level in MPTP mice. The in vitro study demonstrated that lentivirus-mediated CBS overexpression elevated the sulfide generation in glial cells. Moreover, glial CBS overexpression offered protection to midbrain dopaminergic neurons through repressing nitric oxide overproduction in both glial and neuronal cells induced by MPP(+). Taken together, our data suggest

  6. Recovery of fermented inulin fiber by lactic acid bacteria (LAB) from inulin hydrolysate using fungi inulinase enzymes of Scopulariopsis sp.-CBS1 and class of Deuteromycetes-CBS4 as cholesterol binder

    Science.gov (United States)

    Susilowati, Agustine; Melanie, Hakiki; Maryati, Yati; Aspiyanto

    2017-01-01

    Fermentation of Lactobacillus Acid Bacteria (LAB) which are mixtures of Lactobacillus acidophilus, Bifidobacteriumbifidum, Lactobacillus bulgaricus and Streptococcus thermophillus on hydrolysate as a result of inulin hydrolysis using inulinase enzymes obtained from endophytic fungi ofScopulariopsis sp.-CBS1 (inulin hydrolysate of S) and Class of Deuteromycetes-CBS4 (inulin hydrolysate of D) generate potential fermented inulin fiber as cholesterol binder. Fermentation process was conducted under concentrations of inulin hydrolysate 50% (w/v), LAB 15% (v/v) and skim milk 12.5% (w/v) at room temperature and 40°C for 0, 12, 24, 36 and 48 hours, respectively. Result of experimental work showed that longer time of LAB fermentation increased total acids, TPC and CBC at pH 2, but decreased total sugar, reducing, IDF, SDF, CBC pH 2 and CBC pH 7. Based on Cholesterol Binding Capacity (CBC), optimization of fermentation process on inulin hydrolysate of S was achieved by combining treatment at 40°C for 24 hours resulted in CBC pH 2 of 19.11 mg/g TDF and inulin hydrolysate of D was achieved by fermentation at 40 °C for 48 hours resulted in CBC pH 2 of 24.28 mg/g TDF. Inulin hydrolysate of class of Deutrymecetes CBS4 fermented by LAB had better functional property as cholesterol binder than that inulin hydrolysate of S fermented by LAB. This is due to cholesterol binder and cholesterol derivatives as a result of degradation of LAB on digestive system (stomach) when compared to higher colon under optimal process condition.

  7. Gene-environment and gene-gene interactions of specific MTHFR, MTR and CBS gene variants in relation to homocysteine in black South Africans.

    Science.gov (United States)

    Nienaber-Rousseau, Cornelie; Ellis, Suria M; Moss, Sarah J; Melse-Boonstra, Alida; Towers, G Wayne

    2013-11-01

    The methylenetetrahydrofolate reductase (MTHFR), cystathione-β-synthase (CBS) and methionine synthase (MTR) genes interact with each other and the environment. These interactions could influence homocysteine (Hcy) and diseases contingent thereon. We determined single nucleotide polymorphisms (SNPs) within these genes, their relationships and interactions with total Hcy concentrations within black South Africans to address the increased prevalence of diseases associated with Hcy. The MTHFR 677 TT and MTR 2756 AA genotypes were associated with higher Hcy concentrations (16.6 and 10.1 μmol/L; pCBS genotypes did not influence Hcy. We demonstrated interactions between the area of residence and the CBS T833C/844ins68 genotypes (p=0.005) so that when harboring the wildtype allele, rural subjects had significantly higher Hcy than their urban counterparts, but when hosting the variant allele the environment made no difference to Hcy. Between the CBS T833C/844ins68 or G9276A and MTHFR C677T genotypes, there were two-way interactions (p=0.003 and=0.004, respectively), with regard to Hcy. Subjects harboring the MTHFR 677 TT genotype in combination with the CBS 833 TT/homozygous 844 non-insert or the MTHFR 677 TT genotype in combination with the CBS 9276 GA/GG displayed higher Hcy concentrations. Therefore, some of the investigated genotypes affected Hcy; residential area changed the way in which the CBS T833C/844ins68 SNPs influenced Hcy concentrations highlighting the importance of environmental factors; and gene-gene interactions allude to epistatic effects. © 2013 Elsevier B.V. All rights reserved.

  8. The association between CBS 844ins68 polymorphism and head and neck squamous cell carcinoma risk - a case-control analysis.

    Science.gov (United States)

    Galbiatti, Ana L S; Ruiz, Mariangela T; Raposo, Luis S; Maniglia, José V; Pavarino-Bertelli, Erika C; Goloni-Bertollo, Eny M

    2010-10-01

    Susceptibility to head and neck squamous cell carcinoma may be modified by functional polymorphisms in genes involved in the folate pathway, such as cystathionine beta-synthase (CBS). The CBS 844ins68 polymorphism is associated with DNA methylation changes and cancer development. A case-control retrospective study was conducted in 322 patients with head and neck squamous cell carcinoma and in 531 control subjects without cancer. The polymerase chain reaction-restriction fragment length polymorphism technique was used to genotype the polymorphism. For statistical analysis, χ(2) test was conducted to examine whether the genotypic frequency of CBS 844ins68 was in Hardy-Weinberg equilibrium and multiple logistic regression was used for comparisons between groups, and for interactions between the polymorphism and risk factors and clinical histopathological parameters. No significant difference in CBS 844ins68 genotypic distribution was observed between the groups. Age > 50 years, male gender and tobacco consumption were predictors of the disease with increased risk of 7.89 (95% CI: 5.56-11.21), 2.49 (95% CI: 1.72-3.62), 6.44 (95% CI: 4.63-8.96) and 2.29 times (95% CI: 1.71-3.06) respectively. There was no association between the distribution of the CBS 844ins68 genotype and risk factors for this disease. According to clinical histopathological parameters, CBS 884ins68 polymorphism presented high frequency in oral cavity (p CBS 844ins68 polymorphism is not associated with HNSCC risk and there is increased risk of this disease in male gender individuals smokers aged over 50 years. In adittion, the polymorphism is more frequent in patients with oral cavity as primary site and in patients with less survival time.

  9. Determination of Tinopal CBS-X in rice papers and rice noodles using HPLC with fluorescence detection and LC-MS/MS.

    Science.gov (United States)

    Ko, Kyung Yuk; Lee, Chae A; Choi, Jae Chon; Kim, Meehye

    2014-01-01

    To date there have been no reports of methods to determine Tinopal CBS-X. We developed a rapid and simple method to determine the Tinopal CBS-X content in rice noodles and rice papers using HPLC equipped with fluorescence detection. Heating the rice noodles and rice papers to 80°C after adding 75% methanol solution induced the release of Tinopal CBS-X from processed rice products. Tinopal CBS-X was separated using an isocratic mobile phase comprising 50% acetonitrile/water containing 0.4% tetrabutyl ammonium hydrogen sulphate at pH 8.0. The samples suspected to be positive by HPLC analysis were then confirmed by LC-MS/MS analysis. This study also investigated the Tinopal CBS-X content of three rice noodle products and two rice papers. The limits of quantification for rice papers and rice noodles were 1.58 and 1.51 µg kg(-1), respectively, and their correlation curves showed good linearity with r(2) ≥ 0.9997 and ≥ 0.9998, respectively. Moreover, rice papers had recoveries of 70.3-83.3% with precision ranging from 5.0% to 7.9%, whereas rice noodles had slightly lower recoveries of 63.4-78.7% and precisions of 8.5-11.5%. Only one rice noodle product contained Tinopal CBS-X, at around 2.1 mg kg(-1), whereas it was not detected in four other samples. Consequently, Tinopal CBS-X from rice noodles and rice papers can be successfully detected using the developed pre-treatment and ion-pairing HPLC system coupled with fluorescence detection.

  10. Comparative genomics of citric-acid producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88

    Energy Technology Data Exchange (ETDEWEB)

    Andersen, Mikael R.; Salazar, Margarita; Schaap, Peter; van de Vondervoort, Peter; Culley, David E.; Thykaer, Jette; Frisvad, Jens C.; Nielsen, Kristian F.; Albang, Richard; Albermann, Kaj; Berka, Randy; Braus, Gerhard; Braus-Stromeyer, Susanna A.; Corrochano, Luis; Dai, Ziyu; van Dijck, Piet; Hofmann, Gerald; Lasure, Linda L.; Magnuson, Jon K.; Menke, Hildegard; Meijer, Martin; Meijer, Susan; Nielsen, Jakob B.; Nielsen, Michael L.; van Ooyen, Albert; Pel, Herman J.; Poulsen, Lars; Samson, Rob; Stam, Hein; Tsang, Adrian; van den Brink, Johannes M.; ATkins, Alex; Aerts, Andrea; Shapiro, Harris; Pangilinan, Jasmyn; Salamov, Asaf; Lou, Yigong; Lindquist, Erika; Lucas, Susan; Grimwood, Jane; Grigoriev, Igor V.; Kubicek, Christian P.; Martinez, Diego; van Peij, Noel; Roubos, Johannes A.; Nielsen, Jens B.; Baker, Scott E.

    2011-06-01

    The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenicity. Although the genome of an industrial enzyme-producing A. niger strain (CBS 513.88) has already been sequenced, the versatility and diversity of this species compels additional exploration. We therefore undertook whole genome sequencing of the acidogenic A. niger wild type strain (ATCC 1015), and produced a genome sequence of very high quality. Only 15 gaps are present in the sequence and half the telomeric regions have been elucidated. Moreover, sequence information from ATCC 1015 was utilized to improve the genome sequence of CBS 513.88. Chromosome-level comparisons uncovered several genome rearrangements, deletions, a clear case of strain-specific horizontal gene transfer, and identification of 0.8 megabase of novel sequence. Single nucleotide polymorphisms per kilobase (SNPs/kb) between the two strains were found to be exceptionally high (average: 7.8, maximum: 160 SNPs/kb). High variation within the species was confirmed with exo-metabolite profiling and phylogenetics. Detailed lists of alleles were generated, and genotypic differences were observed to accumulate in metabolic pathways essential to acid production and protein synthesis. A transcriptome analysis revealed up-regulation of the electron transport chain, specifically the alternative oxidative pathway in ATCC 1015, while CBS 513.88 showed significant up regulation of genes associated with biosynthesis of amino acids that are abundant in glucoamylase A, tRNA-synthases and protein transporters.

  11. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88

    Science.gov (United States)

    Andersen, Mikael R.; Salazar, Margarita P.; Schaap, Peter J.; van de Vondervoort, Peter J.I.; Culley, David; Thykaer, Jette; Frisvad, Jens C.; Nielsen, Kristian F.; Albang, Richard; Albermann, Kaj; Berka, Randy M.; Braus, Gerhard H.; Braus-Stromeyer, Susanna A.; Corrochano, Luis M.; Dai, Ziyu; van Dijck, Piet W.M.; Hofmann, Gerald; Lasure, Linda L.; Magnuson, Jon K.; Menke, Hildegard; Meijer, Martin; Meijer, Susan L.; Nielsen, Jakob B.; Nielsen, Michael L.; van Ooyen, Albert J.J.; Pel, Herman J.; Poulsen, Lars; Samson, Rob A.; Stam, Hein; Tsang, Adrian; van den Brink, Johannes M.; Atkins, Alex; Aerts, Andrea; Shapiro, Harris; Pangilinan, Jasmyn; Salamov, Asaf; Lou, Yigong; Lindquist, Erika; Lucas, Susan; Grimwood, Jane; Grigoriev, Igor V.; Kubicek, Christian P.; Martinez, Diego; van Peij, Noël N.M.E.; Roubos, Johannes A.; Nielsen, Jens; Baker, Scott E.

    2011-01-01

    The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenicity. Although the genome of an industrial enzyme-producing A. niger strain (CBS 513.88) has already been sequenced, the versatility and diversity of this species compel additional exploration. We therefore undertook whole-genome sequencing of the acidogenic A. niger wild-type strain (ATCC 1015) and produced a genome sequence of very high quality. Only 15 gaps are present in the sequence, and half the telomeric regions have been elucidated. Moreover, sequence information from ATCC 1015 was used to improve the genome sequence of CBS 513.88. Chromosome-level comparisons uncovered several genome rearrangements, deletions, a clear case of strain-specific horizontal gene transfer, and identification of 0.8 Mb of novel sequence. Single nucleotide polymorphisms per kilobase (SNPs/kb) between the two strains were found to be exceptionally high (average: 7.8, maximum: 160 SNPs/kb). High variation within the species was confirmed with exo-metabolite profiling and phylogenetics. Detailed lists of alleles were generated, and genotypic differences were observed to accumulate in metabolic pathways essential to acid production and protein synthesis. A transcriptome analysis supported up-regulation of genes associated with biosynthesis of amino acids that are abundant in glucoamylase A, tRNA-synthases, and protein transporters in the protein producing CBS 513.88 strain. Our results and data sets from this integrative systems biology analysis resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi. PMID:21543515

  12. Yerel zemin özelliklerinin deprem hasarı üzerindeki etkisinin CBS ile değerlendirilmesi

    Directory of Open Access Journals (Sweden)

    Sedat Sert

    2016-12-01

    Full Text Available Orta büyüklükte depremler Sakarya bölgesini 10-30 yıl aralıklarla etkilemektedir. Bunların en sonuncusu olan 1999 depremleri bölgede zemin özelliklerine bağlı olarak farklı hasara neden olmuştur. Bu çalışmada, Sakarya Üniversitesi tarafından farklı zemin özellikleri gösterdiği bilinen Geyve ve Arifiye ilçe merkezlerinde yürütülen arazi ve laboratuvar çalışmalarından elde edilen sonuçlar CBS ortamında değerlendirilmiştir. Araştırma konusu iki ilçede yapılan sondajlar ve koni penetrasyon deneylerinden gelen bilgiler bir veri tabanına işlenmiş ve CBS tabanlı bir yazılımla değerlendirilmiştir. İncelenen 15 m derinlik boyunca tematik haritalar hazırlanarak, deprem sonrası oluşan hasar jeolojik, jeomorfolojik ve geoteknik açıdan değerlendirilmiştir. CBS ortamında hazırlanan haritalar yerel zemin özelliklerinin davranışı etkilediğini göstermekte, artan taşıma gücüyle ağır hasarlı bina sayısının azaldığı görülmektedir. Ayrıca, killi zeminlerin hakim olduğu bölgelerde sıvılaşma hasarının görülmediği teyit edilmektedir.

  13. The mechanism of NO formation from the decomposition of dialkylamino diazeniumdiolates: density functional theory and CBS-QB3 predictions.

    Science.gov (United States)

    Dutton, Andrew S; Fukuto, Jon M; Houk, K N

    2004-02-09

    The mechanism of decomposition of dialkylamino diazeniumdiolate salts, R(2)N[NONO](-)M(+), to form nitric oxide, NO, was explored using theoretical methods. B3LYP/6-311+G(d) density functional theory calculations gave the optimized geometries, and energetics were further evaluated with the high accuracy CBS-QB3 method, when feasible. Relative pK(a) values were estimated using the PCM model for aqueous solvation. The terminal oxygen is the most basic site, followed closely by the internal oxygen. Protonation of these sites does not lead to decomposition. However, protonation of the weakly basic amino nitrogen leads to very rapid decomposition and NO generation.

  14. Mechanisms of HNO and NO production from Angeli's salt: density functional and CBS-QB3 theory predictions.

    Science.gov (United States)

    Dutton, Andrew S; Fukuto, Jon M; Houk, K N

    2004-03-31

    The mechanism of decomposition of Angeli's salt, Na(2)N(2)O(3), was explored with B3LYP and CBS-QB3 computational methods. Angeli's salt produces both nitroxyl (HNO) and nitric oxide (NO), depending upon the pH of the solution. These calculations show that protonation on N(2), while less favorable than O protonation, leads spontaneously to HNO production, while diprotonation at O(3) leads to NO generation. K(a) values for protonation at different centers and rate constants have been found which reproduce experimental data satisfactorily.

  15. Comparative genomics of citric-acid producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88

    Energy Technology Data Exchange (ETDEWEB)

    Grigoriev, Igor V.; Baker, Scott E.; Andersen, Mikael R.; Salazar, Margarita P.; Schaap, Peter J.; Vondervoot, Peter J.I. van de; Culley, David; Thykaer, Jette; Frisvad, Jens C.; Nielsen, Kristen F.; Albang, Richard; Albermann, Kaj; Berka, Randy M.; Braus, Gerhard H.; Braus-Stromeyer, Susanna A.; Corrochano, Luis M.; Dai, Ziyu; Dijck, Piet W.M. van; Hofmann, Gerald; Lasure, Linda L.; Magnusson, Jon K.; Meijer, Susan L.; Nielsen, Jakob B.; Nielsen, Michael L.; Ooyen, Albert J.J. van; Panther, Kathyrn S.; Pel, Herman J.; Poulsen, Lars; Samson, Rob A.; Stam, Hen; Tsang, Adrian; Brink, Johannes M. van den; Atkins, Alex; Aerts, Andrea; Shapiro, Harris; Pangilinan, Jasmyn; Salamov, Asaf; Lou, Yigong; Lindquist, Erika; Lucas, Susan; Grimwood, Jane; Kubicek, Christian P.; Martinez, Diego; Peij, Noel N.M.E. van; Roubos, Johannes A.; Nielsen, Jens

    2011-04-28

    The filamentous fungus Aspergillus niger exhibits great diversity in its phenotype. It is found globally, both as marine and terrestrial strains, produces both organic acids and hydrolytic enzymes in high amounts, and some isolates exhibit pathogenicity. Although the genome of an industrial enzyme-producing A. niger strain (CBS 513.88) has already been sequenced, the versatility and diversity of this species compels additional exploration. We therefore undertook whole genome sequencing of the acidogenic A. niger wild type strain (ATCC 1015), and produced a genome sequence of very high quality. Only 15 gaps are present in the sequence and half the telomeric regions have been elucidated. Moreover, sequence information from ATCC 1015 was utilized to improve the genome sequence of CBS 513.88. Chromosome-level comparisons uncovered several genome rearrangements, deletions, a clear case of strain-specific horizontal gene transfer, and identification of 0.8 megabase of novel sequence. Single nucleotide polymorphisms per kilobase (SNPs/kb) between the two strains were found to be exceptionally high (average: 7.8, maximum: 160 SNPs/kb). High variation within the species was confirmed with exo-metabolite profiling and phylogenetics. Detailed lists of alleles were generated, and genotypic differences were observed to accumulate in metabolic pathways essential to acid production and protein synthesis. A transcriptome analysis revealed up-regulation of the electron transport chain, specifically the alternative oxidative pathway in ATCC 1015, while CBS 513.88 showed significant up-regulation of genes relevant to glucoamylase A production, such as tRNA-synthases and protein transporters. Our results and datasets from this integrative systems biology analysis resulted in a snapshot of fungal evolution and will support further optimization of cell factories based on filamentous fungi.[Supplemental materials (10 figures, three text documents and 16 tables) have been made available

  16. Ethanol reassimilation and ethanol tolerance in Pitchia stipitis CBS 6054 as studied by [sup 13]C nuclear magnetic resonance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Skoog, K.; Hahn-Haegerdal, B. (Univ. of Lund (Sweden)); Degn, H.; Jacobsen, H.S.; Jacobsen, J.P. (Univ. of Odense (Denmark))

    1992-08-01

    Ethanol reassimilation in Pichia stipitis CBS 6054 was studied by using continuous cultures, and the oxidation of [1-[sup 13]C] ethanol was monitored by in vivo and in vitro [sup 13]C nuclear magnetic resonance spectroscopy. Acetate was formed when ethanol was reassimilated. The ATP/ADP ratio and the carbon dioxide production decreased, whereas the malate dehydrogenase activity increased, in ethanol-reassimilating cells. The results are discussed in terms of the low ethanol tolerance in P. stipitis compared with that in Saccharomyces cerevisiae.

  17. Comparative fermentability of enzymatic and acid hydrolysates of steam-pretreated aspenwood hemicellulose by Pichia stipitis CBS 5776

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, J.J.; Deschatelets, L.; Nishikawa, N.K. (Forintek Canada Corp., Ottawa, ON (Canada). Biotechnology and Chemistry Dept.)

    1989-10-01

    Enzymatic hydrolysates of hemicellulose from steam-pretreated aspenwood were more fermentable than the acid hydrolysate after rotoevaporation or ethyl acetate extraction treatments to remove acetic acid and sugar- and lignin-degradation products prior to fermentation by Pichia stipitis CBS 5776. Total xylose and xylobiose utilization from 5.0% (w/v) ethyl acetate extracted enzymatic hydrolysate was observed with an ethanol yield of 0.47 g ethanol/g total available substrate and an ethanol production rate of 0.20 gxl{sup -1} per hour in 72 h batch fermentation. (orig.).

  18. Havza morfolojik özelliklerinin belirlenmesinde coğrafi bilgi sistemlerinin (CBS etkin rolü

    Directory of Open Access Journals (Sweden)

    Cüneyt Bağdatlı

    2014-04-01

    Full Text Available Bu çalışmada, sayısal yükseklik modelleri üzerinden havza morfolojik özelliklerinin belirlenmesinde Coğrafi BilgiSistemlerinin (CBS etkin rolünün ortaya konulması amaçlanmıştır. Bu bağlamda havza karakteristik özelliklerinden;havza sınırı, alanı, çevresi, akış yönleri, ağırlık merkezleri ile drenaj ağları, eğim ve bakı özellikleri belirlenmiştir.Çalışmada 1\\25.000 ölçekli sayısal topografik haritalar üzerinden CBS ortamında materyal olarak seçilen Çorlu Deresihavza alanına ilişkin tüm havza karakteristik özellikleri belirlenmiş ve havza alanına ilişkin temel altlığı oluşturacaksayısal yükseklik modelleri ortaya konulmuştur. Araştırma kapsamında, Arc GIS 10.2 CBS yazılımının Spatial Analystmodülünden yararlanılmıştır. Sayısal topografik harita üzerinden klasik yöntemlerle belirlenmesi zor ve zaman alıcıolan havza sınırlarının ve gerekli morfolojik özelliklerinin CBS yardımıyla oluşturulacak sayısal yükseklik modelikullanılarak ne kadar kolay ve hızlı bir şekilde yapılabileceğine ilişkin etkin yaklaşımlar ortaya konulmuştur.

  19. Low sulfide levels and a high degree of cystathionine β-synthase (CBS) activation by S-adenosylmethionine (SAM) in the long-lived naked mole-rat.

    Science.gov (United States)

    Dziegelewska, Maja; Holtze, Susanne; Vole, Christiane; Wachter, Ulrich; Menzel, Uwe; Morhart, Michaela; Groth, Marco; Szafranski, Karol; Sahm, Arne; Sponholz, Christoph; Dammann, Philip; Huse, Klaus; Hildebrandt, Thomas; Platzer, Matthias

    2016-08-01

    Hydrogen sulfide (H2S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS), an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  20. Low sulfide levels and a high degree of cystathionine β-synthase (CBS activation by S-adenosylmethionine (SAM in the long-lived naked mole-rat

    Directory of Open Access Journals (Sweden)

    Maja Dziegelewska

    2016-08-01

    Full Text Available Hydrogen sulfide (H2S is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS, an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration.

  1. Effects of B2O3 content and sintering temperature on crystallization and microstructure of CBS glass-ceramic coatings

    Science.gov (United States)

    Li, Pengyang; Wang, Shubin; Liu, Jianggao; Feng, Mengjie; Yang, Xinwang

    2015-11-01

    Borosilicate glass-ceramics precursors with varying compositional ratios in the CaO-SiO2-B2O3 (CBS) system were synthesized by sol-gel method. The precursors were calcined at 1200 °C for 2 h to form glass powders. The glass-ceramics were prepared by overlaying glass slurries on the substrates before sintering at different temperatures. The as-prepared glasses and glass-ceramics were characterized by differential scanning calorimetry and X-ray diffraction. The crystallization activation energies (Ec) were calculated using the Kissinger method from DSC results. The morphology and crystallization behavior of the glass-ceramics were monitored by scanning electron microscopy. Both glass transition and crystallization temperatures decreased, however, the metastable zone increased. The Ec values of CBS glasses and glass-ceramics were 254.1, 173.2 and 164.4 kJ/mol with increasing B2O3 content, whereas that of the calcined G3 glass was 104.9 kJ/mol. Finally, the coatings were prepared at a low temperature (700 °C). The crystals that grew on the surface of multilayer coatings demonstrated heterogeneous surface nucleation and crystallization after heat-treatment from 700 °C to 850 °C for 4 h.

  2. Rs6586282 of the CBS Gene: Its Lack of Eff ect on Homocysteine Concentrations, and Interaction Eff ects on Body Weight in Elderly Women.

    Science.gov (United States)

    Chmurzynska, Agata; Malinowska, Anna M; Twardowska-Rajewska, Jolanta; Gawecki, Jan

    2017-08-15

    The aim of the present study is to evaluate the effect of the rs6586282 polymorphism of the cystathionine-β-synthase (CBS) gene, and of the intake of B vitamins on anthropometric parameters, tHcy levels, and lipoprotein levels in women over 60 years of age. 122 volunteers were supplemented with 400 μg/day folic acid for 8 weeks. The intake of B vitamins above the median value was associated with lower levels of blood biomarkers: folate with tHcy post supplementation (6.21 ± 0.24 μM vs 7.11 ± 0.32 μM; p CBS gene does not alter either tHcy or the effectiveness of folic acid supplementation. The CBS SNP at rs6586282 may infl uence anthropometric parameters, though only in case of low folate intake.

  3. Comparative Proteome Analysis between High Lipid-Producing Strain Mucor circinelloides WJ11 and Low Lipid-Producing Strain CBS 277.49.

    Science.gov (United States)

    Tang, Xin; Chen, Haiqin; Gu, Zhennan; Zhang, Hao; Chen, Yong Q; Song, Yuanda; Chen, Wei

    2017-06-21

    Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis. Proteome analysis demonstrated that the branched-chain amino acid and lysine metabolism, glycolytic pathway, and pentose phosphate pathway in WJ11 were up-regulated, while the activities of tricarboxylic acid cycle and branch point enzyme for synthesis of isoprenoids were retarded compared with CBS 277.49. The coordinated regulation at proteome level indicate that more acetyl-CoA and NADPH are provided for fatty acid biosynthesis in WJ11 compared with CBS 277.49.

  4. Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L. Heynh and Oryza sativa L. reveals their developmental and stress regulation

    Directory of Open Access Journals (Sweden)

    Sopory Sudhir K

    2009-04-01

    Full Text Available Abstract Background In Arabidopsis thaliana (L. Heynh and Oryza sativa L., a large number of genes encode proteins of unknown functions, whose characterization still remains one of the major challenges. With an aim to characterize these unknown proteins having defined features (PDFs in plants, we have chosen to work on proteins having a cystathionine β-synthase (CBS domain. CBS domain as such has no defined function(s but plays a regulatory role for many enzymes and thus helps in maintaining the intracellular redox balance. Its function as sensor of cellular energy has also been widely suggested. Results Our analysis has identified 34 CBS domain containing proteins (CDCPs in Arabidopsis and 59 in Oryza. In most of these proteins, CBS domain coexists with other functional domain(s, which may indicate towards their probable functions. In order to investigate the role(s of these CDCPs, we have carried out their detailed analysis in whole genomes of Arabidopsis and Oryza, including their classification, nomenclature, sequence analysis, domain analysis, chromosomal locations, phylogenetic relationships and their expression patterns using public databases (MPSS database and microarray data. We have found that the transcript levels of some members of this family are altered in response to various stresses such as salinity, drought, cold, high temperature, UV, wounding and genotoxic stress, in both root and shoot tissues. This data would be helpful in exploring the so far obscure functions of CBS domain and CBS domain-containing proteins in plant stress responses. Conclusion We have identified, classified and suggested the nomenclature of CDCPs in Arabidopsis and Oryza. A comprehensive analysis of expression patterns for CDCPs using the already existing transcriptome profiles and MPSS database reveals that a few CDCPs may have an important role in stress response/tolerance and development in plants, which needs to be validated further through

  5. Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation.

    Science.gov (United States)

    Kushwaha, Hemant R; Singh, Anil K; Sopory, Sudhir K; Singla-Pareek, Sneh L; Pareek, Ashwani

    2009-04-28

    In Arabidopsis thaliana (L.) Heynh and Oryza sativa L., a large number of genes encode proteins of unknown functions, whose characterization still remains one of the major challenges. With an aim to characterize these unknown proteins having defined features (PDFs) in plants, we have chosen to work on proteins having a cystathionine beta-synthase (CBS) domain. CBS domain as such has no defined function(s) but plays a regulatory role for many enzymes and thus helps in maintaining the intracellular redox balance. Its function as sensor of cellular energy has also been widely suggested. Our analysis has identified 34 CBS domain containing proteins (CDCPs) in Arabidopsis and 59 in Oryza. In most of these proteins, CBS domain coexists with other functional domain(s), which may indicate towards their probable functions. In order to investigate the role(s) of these CDCPs, we have carried out their detailed analysis in whole genomes of Arabidopsis and Oryza, including their classification, nomenclature, sequence analysis, domain analysis, chromosomal locations, phylogenetic relationships and their expression patterns using public databases (MPSS database and microarray data). We have found that the transcript levels of some members of this family are altered in response to various stresses such as salinity, drought, cold, high temperature, UV, wounding and genotoxic stress, in both root and shoot tissues. This data would be helpful in exploring the so far obscure functions of CBS domain and CBS domain-containing proteins in plant stress responses. We have identified, classified and suggested the nomenclature of CDCPs in Arabidopsis and Oryza. A comprehensive analysis of expression patterns for CDCPs using the already existing transcriptome profiles and MPSS database reveals that a few CDCPs may have an important role in stress response/tolerance and development in plants, which needs to be validated further through functional genomics.

  6. STATUS GIZI DAN FUNGSI HATI MENCIT ( GALUR CBS-SWISS DAN TIKUS PUTIH (GALUR WISTAR DI LABORATORIUM HEWAN PERCOBAAN PUSLITBANG BIOMEDIS DAN FARMASI

    Directory of Open Access Journals (Sweden)

    Marice Sihombing

    2012-12-01

    Full Text Available Mice (CBS-Swiss strain and albino rats (Wistar strain are experimental animals bred and kept in Centre for Biomedical and Pharmaceuticals Research and Development, National Institute of Health Research and Development (NIHRD Jakarta. The objective of this study is to elucidate the health  profile of these animals and to assess their suitability as experimental animals. Parameters being measured include body weight gain, histopathology of the liver, blood test (SGPT, SCOT, billirubin scale and internal organ weights (liver, kidney, lungs and lymph nodes. In this study, particularly forthe body weight, used 180 mice and 180 rats, divided into 3 age groups 1, 2 and 3 months old. Each age groups consisted of 60 animals (30 male and 30 female. For the measurement of blood test, weight of internal organs and histopathologic of the liver examination used 60 mice and 60 rats from these animals. Which are divided into 3 groups 1, 2 and 3 months old, each groups were composed of 20 (10 male and 10 female animals. Mice and rats are taken at random sampling. Data analysis were conducted by ANOVA one-way test, except for organ weight (liver and lungs for mice and liver foralbino rats where data were analysed using a non-parametric test. Linear regression test was conducted for measurement of body weight gain in each age group. The result of the study suggested that the weight gain in mice and albino rats correlate with increasing of age. Histopathological assessment of liver did not show any cell damage or degeneration of parenchyma cells in both species.The blood test also showed normal values, and in general the organ weights (liver, kidney, lungs and lymph nodes showed an increase that correlated with age. The study conclude that in general the conditions of these animals and their physiological state are good, and they are fit to be used as experimental animals. Keywords: mice, albino rats, body weight, blood test parameters, liver

  7. 十六烷基三甲基溴化铵增敏荧光光度法测定荧光增白剂CBS-X%Determination of Fluorescent Whitening Agent CBS-X by Sensitization Fluorescent Spectrophotometry with Cetyl Trimethyl Ammonium Bromide

    Institute of Scientific and Technical Information of China (English)

    潘可亮; 李树伟; 杨利

    2011-01-01

    The fluorescence characteristics of fluorescent whitening agent CBS-X in different medium and the sensitization mechanism of cetyl trimethyl ammonium bromide (CTAB) to CBS-X have been studied. A method on sensitization fluorescence determinations of the CBS -X was therefore developed. This method has the advantages of easy handle of the specimen, quickly test and low detection limit. The method has been used for the detection of synthetic detergent with satisfactory results. The linear regression equation is If = 1. 31+ 2. 85×109c (mol/L) with a correlation coefficient (R) of 0. 9995 and the linear range from 0 to 1. 73 ×10-7mol/L. The detection limit is 0. 45 ng/mL.%研究了不同条件下荧光增白剂CBS-X的荧光光谱,探讨了十六烷基三甲基溴化铵(CTAB)对荧光增白剂CBS-X荧光的增敏机理.建立了在pH=8的Tris-HCl缓冲溶液中,CTAB增敏荧光光度法测定CBS -X含量的新方法.方法的线性范围为0~1.73×10-7 mol/L,相关系数为0.9995,检出限为0.45 ng/mL.该方法用于合成洗涤剂中CBS-X的测定,得到满意结果.

  8. Synthesis of R-3-quinine alcohol by CBS catalysis%CBS催化合成R-3-奎宁醇

    Institute of Scientific and Technical Information of China (English)

    任彦荣

    2012-01-01

    The chiral synthesis technique was applied to prepare R-3-quinine alcohol by catalytic reduction of 3-quinine ketone with the help of CBS catalyst. The yield and ee value were above 92% and 98% .respectively. The proposed synthesis method appeared to be convenient, high-yield, and applicable for industrial purpose.%探讨R-3-奎宁醇的合成工艺.通过手性合成的方法,以3-奎宁酮盐酸盐为原料,经CBS催化剂催化还原得R-3-奎宁醇.收率可达到92%以上,ee值可达98%以上.该合成路线操作步骤简单、收率高,具有工业应用价值.

  9. Detoxification of corn stover prehydrolyzate by trialkylamine extraction to improve the ethanol production with Pichia stipitis CBS 5776.

    Science.gov (United States)

    Zhu, Junjun; Yong, Qiang; Xu, Yong; Yu, Shiyuan

    2011-01-01

    In order to realize the separated ethanol fermentation of glucose and xylose, prehydrolysis of corn stover with sulfuric acid at moderate temperature was applied, while inhibitors were produced inevitably. A complex extraction was adopted to detoxify the prehydrolyzate before fermentation to ethanol with Pichia stipitis CBS 5776. The best proportion of mixed extractant was 30% trialkylamine-50% n-octanol -20% kerosene. Detoxification results indicated that 73.3% of acetic acid, 45.7% of 5-hydroxymethylfurfural and 100% of furfural could be removed. Compared with the undetoxified prehydrolyzate, the fermentability of the detoxified prehydrolyzate was significantly improved. After 48 h fermentation of the detoxified prehydrolyzate containing 7.80 g/l of glucose and 52.8 g/l of xylose, the sugar utilization ratio was 93.2%; the ethanol concentration reached its peak value of 21.8 g/l, which was corresponding to 82.3% of the theoretical value.

  10. Nitrile bioconversion by Microbacterium imperiale CBS 498-74 resting cells in batch and ultrafiltration membrane bioreactors.

    Science.gov (United States)

    Cantarella, M; Cantarella, L; Gallifuoco, A; Spera, A

    2006-03-01

    The biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the NHase activity contained in resting cells of Microbacterium imperiale CBS 498-74 was operated at 5, 10 and 20 degrees C in laboratory-scale batch and membrane bioreactors. The bioreactions were conducted in buffered medium (50 mM Na(2)HPO(4)/NaH(2)PO(4), pH 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. The integral bioreactor performances were studied with a cell loading (dry cell weight; DCW) varying from 0.1 mg(DCW) per reactor to 16 mg(DCW) per reactor, in order to realize near 100% bioconversion of acrylonitrile, propionitrile and benzonitrile without consistent loss of NHase activity.

  11. Over-Expression of Rice CBS Domain Containing Protein, OsCBSX3, Confers Rice Resistance to Magnaporthe oryzae Inoculation.

    Science.gov (United States)

    Mou, Shaoliang; Shi, Lanping; Lin, Wei; Liu, Yanyan; Shen, Lei; Guan, Deyi; He, Shuilin

    2015-07-13

    Cystathionine β-synthase (CBS) domain containing proteins (CDCPs) constitute a big family in plants and some members in this family have been implicated in a variety of biological processes, but the precise functions and the underlying mechanism of the majority of this family in plant immunity remain to be elucidated. In the present study, a CBS domain containing protein gene, OsCBSX3, is functionally characterized in rice resistance against Magnaporthe oryzae (M. oryzae). By quantitative real-time PCR, transcripts of OsCBSX3 are up-regulated significantly by inoculation of M. oryzae and the exogenously applied salicylic acid (SA) and methyl jasmonate (MeJA). OsCBSX3 is exclusively localized to the plasma membrane by transient expression of OsCBSX3 fused to green fluorescent protein (GFP) through approach of Agrobacterium infiltration in Nicotiana benthamiana leaves. The plants of homozygous T3 transgenic rice lines of over-expressing OsCBSX3 exhibit significant enhanced resistance to M. oryzae inoculation, manifested by decreased disease symptoms, and inhibition of pathogen growth detected in DNA. Consistently, the over-expression of OsCBSX3 enhances the transcript levels of immunity associated marker genes including PR1a, PR1b, PR5, AOS2, PAL, NH1, and OsWRKY13 in plants inoculated with M. oryzae. These results suggest that OsCBSX3 acts as a positive regulator in resistance of rice to M. oryzae regulated by SA and JA-mediated signaling pathways synergistically.

  12. Over-Expression of Rice CBS Domain Containing Protein, OsCBSX3, Confers Rice Resistance to Magnaporthe oryzae Inoculation

    Directory of Open Access Journals (Sweden)

    Shaoling Mou

    2015-07-01

    Full Text Available Cystathionine β-synthase (CBS domain containing proteins (CDCPs constitute a big family in plants and some members in this family have been implicated in a variety of biological processes, but the precise functions and the underlying mechanism of the majority of this family in plant immunity remain to be elucidated. In the present study, a CBS domain containing protein gene, OsCBSX3, is functionally characterized in rice resistance against Magnaporthe oryzae (M. oryzae. By quantitative real-time PCR, transcripts of OsCBSX3 are up-regulated significantly by inoculation of M. oryzae and the exogenously applied salicylic acid (SA and methyl jasmonate (MeJA. OsCBSX3 is exclusively localized to the plasma membrane by transient expression of OsCBSX3 fused to green fluorescent protein (GFP through approach of Agrobacterium infiltration in Nicotiana benthamiana leaves. The plants of homozygous T3 transgenic rice lines of over-expressing OsCBSX3 exhibit significant enhanced resistance to M. oryzae inoculation, manifested by decreased disease symptoms, and inhibition of pathogen growth detected in DNA. Consistently, the over-expression of OsCBSX3 enhances the transcript levels of immunity associated marker genes including PR1a, PR1b, PR5, AOS2, PAL, NH1, and OsWRKY13 in plants inoculated with M. oryzae. These results suggest that OsCBSX3 acts as a positive regulator in resistance of rice to M. oryzae regulated by SA and JA-mediated signaling pathways synergistically.

  13. Novel alleles of 31-bp VNTR polymorphism in the human cystathionine -synthase (CBS) gene were detected in healthy Asians

    Indian Academy of Sciences (India)

    Yik-Yuen Gan; Chuan-Fei Chen

    2010-12-01

    A 31-bp variable number of tandem repeats (VNTR) polymorphism of the cystathionine -synthase (CBS) gene was earlier reported in Caucasians of predominantly European descent and Indo–Caucasoid populations.We report here for the first time, the detection of allele 20, which was absent in Caucasian and Indo–Caucasoid populations, as a common allele present in Singaporean Chinese (6.25%), Indians (11.7%), and Malays (11.5%). Hence, allele 20 might be a specific allele for Asian populations. A relatively common allele 19 found in the Caucasian and Indo–Caucasoid populations (10.4%–10.6%) was absent in the Asian samples of this study. Therefore, allele 19 might be a specific allele for the Caucasian populations. A novel and rare allele 13, which was not reported before in the Caucasian and Indo–Caucasoid populations, was found in 0.5% of Singaporean Chinese as genotype 13/17 heterozygotes. The presence of alleles 13 and 20 were verified by DNA sequencing. There were five new genotypes (13/17, 16/20, 17/20, 18/20 and 20/20) not reported before in the Caucasian and Indo–Caucasoid populations, detected in this study. Nine genotypes (15/18, 16/18, 16/21, 17/19, 18/19, 18/21, 19/19, 19/21 and 21/21) which were present in the Caucasian and/or Indo–Caucasoid populations were absent in this study. Our results showed that CBS 31-bp VNTR polymorphism has a distinct genetic difference in allele and genotype frequencies between the European Caucasians, Indo–Caucasoid and Asian populations.

  14. Growth of the yeast Kluyveromyces marxianus CBS 6556 on different sugar combinations as sole carbon and energy source.

    Science.gov (United States)

    Fonseca, Gustavo Graciano; de Carvalho, Nuno Miguel Barbosa; Gombert, Andreas Karoly

    2013-06-01

    The yeast Kluyveromyces marxianus has been pointed out as a promising microorganism for a variety of industrial bioprocesses. Although genetic tools have been developed for this yeast and different potential applications have been investigated, quantitative physiological studies have rarely been reported. Here, we report and discuss the growth, substrate consumption, metabolite formation, and respiratory parameters of K. marxianus CBS 6556 during aerobic batch bioreactor cultivations, using a defined medium with different sugars as sole carbon and energy source, at 30 and 37 °C. Cultivations were carried out both on single sugars and on binary sugar mixtures. Carbon balances closed within 95 to 101 % in all experiments. Biomass and CO2 were the main products of cell metabolism, whereas by-products were always present in very low proportion (<3 % of the carbon consumed), as long as full aerobiosis was guaranteed. On all sugars tested as sole carbon and energy source (glucose, fructose, sucrose, lactose, and galactose), the maximum specific growth rate remained between 0.39 and 0.49 h(-1), except for galactose at 37 °C, which only supported growth at 0.31 h(-1). Different growth behaviors were observed on the binary sugar mixtures investigated (glucose and lactose, glucose and galactose, lactose and galactose, glucose and fructose, galactose and fructose, fructose and lactose), and the observations were in agreement with previously published data on the sugar transport systems in K. marxianus. We conclude that K. marxianus CBS 6556 does not present any special nutritional requirements; grows well in the range of 30 to 37 °C on different sugars; is capable of growing on sugar mixtures in a shorter period of time than Saccharomyces cerevisiae, which is interesting from an industrial point of view; and deviates tiny amounts of carbon towards metabolite formation, as long as full aerobiosis is maintained.

  15. Deciphering the hybridisation history leading to the Lager lineage based on the mosaic genomes of Saccharomyces bayanus strains NBRC1948 and CBS380.

    Directory of Open Access Journals (Sweden)

    Huu-Vang Nguyen

    Full Text Available Saccharomyces bayanus is a yeast species described as one of the two parents of the hybrid brewing yeast S. pastorianus. Strains CBS380(T and NBRC1948 have been retained successively as pure-line representatives of S. bayanus. In the present study, sequence analyses confirmed and upgraded our previous finding: S. bayanus type strain CBS380(T harbours a mosaic genome. The genome of strain NBRC1948 was also revealed to be mosaic. Both genomes were characterized by amplification and sequencing of different markers, including genes involved in maltotriose utilization or genes detected by array-CGH mapping. Sequence comparisons with public Saccharomyces spp. nucleotide sequences revealed that the CBS380(T and NBRC1948 genomes are composed of: a predominant non-cerevisiae genetic background belonging to S. uvarum, a second unidentified species provisionally named S. lagerae, and several introgressed S. cerevisiae fragments. The largest cerevisiae-introgressed DNA common to both genomes totals 70kb in length and is distributed in three contigs, cA, cB and cC. These vary in terms of length and presence of MAL31 or MTY1 (maltotriose-transporter gene. In NBRC1948, two additional cerevisiae-contigs, cD and cE, totaling 12kb in length, as well as several smaller cerevisiae fragments were identified. All of these contigs were partially detected in the genomes of S. pastorianus lager strains CBS1503 (S. monacensis and CBS1513 (S. carlsbergensis explaining the noticeable common ability of S. bayanus and S. pastorianus to metabolize maltotriose. NBRC1948 was shown to be inter-fertile with S. uvarum CBS7001. The cross involving these two strains produced F1 segregants resembling the strains CBS380(T or NRRLY-1551. This demonstrates that these S. bayanus strains were the offspring of a cross between S. uvarum and a strain similar to NBRC1948. Phylogenies established with selected cerevisiae and non-cerevisiae genes allowed us to decipher the complex hybridisation

  16. Accurate reaction barrier heights of pericyclic reactions: Surprisingly large deviations for the CBS-QB3 composite method and their consequences in DFT benchmark studies.

    Science.gov (United States)

    Karton, Amir; Goerigk, Lars

    2015-04-05

    Accurate barrier heights are obtained for the 26 pericyclic reactions in the BHPERI dataset by means of the high-level Wn-F12 thermochemical protocols. Very often, the complete basis set (CBS)-type composite methods are used in similar situations, but herein it is shown that they in fact result in surprisingly large errors with root mean square deviations (RMSDs) of about 2.5 kcal mol(-1). In comparison, other composite methods, particularly G4-type and estimated coupled cluster with singles, doubles, and quasiperturbative triple excitations [CCSD(T)/CBS] approaches, show deviations well below the chemical-accuracy threshold of 1 kcal mol(-1). With the exception of SCS-MP2 and the herein newly introduced MP3.5 approach, all other tested Møller-Plesset perturbative procedures give poor performance with RMSDs of up to 8.0 kcal mol(-1). The finding that CBS-type methods fail for barrier heights of these reactions is unexpected and it is particularly troublesome given that they are often used to obtain reference values for benchmark studies. Significant differences are identified in the interpretation and final ranking of density functional theory (DFT) methods when using the original CBS-QB3 rather than the new Wn-F12 reference values for BHPERI. In particular, it is observed that the more accurate Wn-F12 benchmark results in lower statistical errors for those methods that are generally considered to be robust and accurate. Two examples are the PW6B95-D3(BJ) hybrid-meta-general-gradient approximation and the PWPB95-D3(BJ) double-hybrid functionals, which result in the lowest RMSDs of the entire DFT study (1.3 and 1.0 kcal mol(-1), respectively). These results indicate that CBS-QB3 should be applied with caution in computational modeling and benchmark studies involving related systems.

  17. ANÁLISE DA EXECUÇÃO DE UM ALGORITMO NUMÉRICO CBS PARA SIMULAÇÃO DE ESCOAMENTO UTILIZANDO ELEMENTOS FINITOS ANALYSIS OF CBS NUMERICAL ALGORITHM EXECUTION TO FLOW SIMULATION USING THE FINITE ELEMENT METHOD

    Directory of Open Access Journals (Sweden)

    Marcos Paulo de Carvalho Oliveira

    2009-08-01

    Full Text Available Neste trabalho é realizada uma análise da aplicação e do tempo de execução de um algoritmo numérico desenvolvido para a simulação de escoamento isotérmico de fluido incompressível. No código, emprega-se o algoritmo denominado esquema baseado nas características (CBS - characteristic based split, em que é usado o acoplamento da compressibilidade artificial (AC para as equações da pressão e velocidades. A discretização foi realizada com o método dos elementos finitos, empregando-se uma malha de elementos bilineares. O programa GNU-Octave foi utilizado para implementar e executar as rotinas. Por meio do problema clássico da cavidade recirculante, foram avaliados resultados para diversos valores do número de Reynolds. Esses resultados mostraram boa concordância quando comparados aos disponíveis na literatura. A análise do tempo de execução das rotinas mostrou ainda que a montagem das matrizes é a etapa que exige maior consumo de tempo da implementação.This work intends to analyze the application and execution time of a numerical algorithm that simulates incompressible and isothermal flows. It was used the explicit scheme of the "Characteristic Based Split" (CBS algorithm and the "Artificial Compressibility" (AC scheme for coupling pressure-velocity equations. The discretization was done with the finite element method using a bilinear elements grid. The free software GNU-Octave was used for implementation and execution of routines. The results were analyzed using the classic lid-driven cavity problem. This work shows results for tests with several Reynolds' number. The results for these tests show a good agreement when compared with previous ones obtained from bibliography. The code runtime's analysis shows yet that the matrix's assembly is the part of greater consumption time in the implementation.

  18. Calcitriol prevents peripheral RSC96 Schwann neural cells from high glucose & methylglyoxal-induced injury through restoration of CBS/H2S expression.

    Science.gov (United States)

    Zhang, Hui; Zhuang, Xiao-dong; Meng, Fu-hui; Chen, Li; Dong, Xiao-bian; Liu, Guo-Hui; Li, Jian-hua; Dong, Qi; Xu, Ji-de; Yang, Chun-tao

    2016-01-01

    A meta-analysis has suggested that vitamin D deficiency is involved in diabetic peripheral neuropathy (DPN) and the levels of hydrogen sulfide (H2S) are also decreased in type 2 diabetes. The injection of vitamin D induces cystathionine-β-synthase (CBS) expression and H2S generation. However, it remains unclear whether the supplementation of vitamin D prevents DPN through improvement of CBS/H2S expression. In the present study, RSC96 cells, a rat Schwann cell line, were exposed to high glucose and methylglyoxal (HG&MG) to simulate diabetic peripheral nerve injury in vivo. Before the exposure to HG&MG, the cells were preconditioned with calcitriol (CCT), an active form of vitamin D, and then CCT-mediated neuroprotection was investigated in respect of cellular viability, superoxide anion (O2(-)) generation, inducible nitric oxide (NO) synthase (iNOS)/NO expression, mitochondrial membrane potential (MMP), as well as CBS expression and activity. It was found that both high glucose and MGO decreased cell viability and co-treatment with the two induced a more serious injury in RSC96 cells. Therefore, the exposure to HG&MG was used in the present study. The exposure to HG&MG markedly induced iNOS expression, NO and O2(-) generation, as well as MMP loss. In addition, the exposure to HG&MG depressed CBS expression and activity in RSC96 cells. However, the preconditioning with CCT significantly antagonized HG&MG-induced cell injury including the decreased viability, iNOS overexpression, NO and O2(-) accumulation, as well as MMP loss. CCT also partially restored the decreased CBS expression and activity triggered by HG&MG, while the inhibition of CBS with hydroxylamine attenuated CCT-mediated neuroprotection. Moreover, the exogenous donation of H2S produced similar cellular protective effects to CCT. The data indicate that the supplementation of vitamin D prevents HG&MG-induced peripheral nerve injury involving the restoration of endogenous H2S system, which may provide a

  19. Mapping of DNA Hypermethylation and Hypomethylation induced by Folate Deficiency in Sporadic Colorectal Cancer and Clinical Implication Analysis of Hypermethylation Pattern in CBS Promoter.

    Science.gov (United States)

    Zhang, Zaizhong; He, Yang; Tu, Xiaohuang; Huang, Sheng; Chen, Zhuo; Wang, Lie; Song, Jingxiang

    2017-04-01

    Aberrant DNA methylation patterns play a major role in tumorigenesis and the effects of nutrients, especially folate in the diet, on methylation changes is of great importance in colorectal cancer (CRC). Folate deficiency would disrupt methylation patterns; however, its exact effects on DNA methylation patterns in CRC are unclear. This study was performed to gain insight into the methylation changes induced by folate deficiency and the putative role of methylation pattern diversities of related genes in the clinical outcome of CRC. The NimbleGen MeDIP chip (Methylated DNA Immunoprecipitation chip) assay was used in high-resolution mapping of DNA methylation patterns in the normal human colon mucosal epithelial cell line, NCM460 cultured with or without folate. Aberrant CpG island methylation patterns in the promoter of genes were identified by chip assay and then were confirmed in paired colorectal tissues and corresponding non-malignant tissues obtained from patients by bisulfate sequencing PCR (BSP). Of the total, the expression of cystathionine-beta-synthase (CBS) involved in methyl metabolism and its important substrate, homocysteine, were all detected by realtime RT-PCR and immunostaining. We also analyzed the data of its hypermethylation level statistically correlated with pathological parameters and the clinical outcome in malignant tissues. The chip assay showed that there are 17 genes with hyper or hypomethylation in CpG islands of promoter on chromosome 21, and 8 of them seemed to be associated with tumorigenesis. Among the total, a hypermethylation patterns existed in the promoter of CBS in CRC (p CBS and the accumulation of homocysteine in vitro and vivo (p CBS hypermethylation level is correlated with age (p CBS hypermethylation level significantly correlated with recurrence rate (p = 0.039) and overall survival (p = 0.012) independent of pT stage, pN stage, and liver metastasis. Folate deficiency could induce aberrant DNA methylation patterns and gene

  20. The CBS-The Most Cost Effective and High Performance Carbon Beam Source Dedicated for a New Generation Cancer Therapy

    CERN Document Server

    Kumada, Masayuki; Leivichev, E B; Parkhomchuk, Vasily; Podgorny, Fedor; Rastigeev, Sergey; Reva, Vladimir B; Skrinsky, Aleksander Nikolayevich; Vostrikov, Vladimir

    2005-01-01

    A Carbon ion beam is a superior tool to x-rays or a proton beam in both physical and biological doses in treating a cancer. A Carbon beam has an advantage in treating radiation resistant and deep-seated tumors. Its radiological effect is of a mitotic independent nature. These features improve hypofractionation, typically reducing the number of irradiations per patient from 35 to a few. It has been shown that a superior QOL(Quality Of Life) therapy is possible by a carbon beam.The only drawback is its high cost. Nevertheless, tens of Prefectures and organizations are eagerly considering the possibility of having a carbon ion therapy facility in Japan. Germany, Austria, Italy, China, Taiwan and Korea also desire to have one.A carbon beam accelerator of moderate cost is about 100 Million USD. With the "CBS" design philosophy, which will be described in this paper, the cost could be factor of 2 or 3 less, while improving its performance more than standard designs. Novel extraction techniques, a new approach to a ...

  1. Stabilized MLPG-VF-based method with CBS scheme for laminar flow at high Reynolds and Rayleigh numbers

    Science.gov (United States)

    Enjilela, Vali; Salimi, Davood; Tavasoli, Ali; Lotfi, Mohsen

    2016-02-01

    In the present work, the meshless local Petrov-Galerkin vorticity-stream function (MLPG-VF) method is extended to solve two-dimensional laminar fluid flow and heat transfer equations for high Reynolds and Rayleigh numbers. The characteristic-based split (CBS) scheme which uses unity test function is employed for discretization, and the moving least square (MLS) method is used for interpolation of the field variables. Four test cases are considered to evaluate the present algorithm, namely lid-driven cavity flow with Reynolds numbers up to and including 104, flow over a backward-facing step at Reynolds number of 800, natural convection in a square cavity for Rayleigh numbers up to and including 108, and natural convection in a concentric square outer cylinder and circular inner cylinder annulus for Rayleigh numbers up to and including 107. In each case, the result obtained using the proposed algorithm is either compared with the results from the literatures or with those obtained using conventional numerical techniques. The present algorithm shows stable results at lower or equal computational cost compared to the other upwinding schemes usually employed in the MLPG method. Close agreements between the compared results as well as higher accuracy of the proposed method show the ability of this stabilized algorithm.

  2. Transcriptome of the alternative ethanol production strain Dekkera bruxellensis CBS 11270 in sugar limited, low oxygen cultivation.

    Science.gov (United States)

    Tiukova, Ievgeniia A; Petterson, Mats E; Tellgren-Roth, Christian; Bunikis, Ignas; Eberhard, Thomas; Pettersson, Olga Vinnere; Passoth, Volkmar

    2013-01-01

    Dekkera bruxellensis can outcompete Saccharomyces cerevisiae in environments with low sugar concentrations. It is usually regarded as a spoilage yeast but has lately been identified as an alternative ethanol production organism. In this study, global gene expression in the industrial isolate D. bruxellensis CBS 11270 under oxygen and glucose limitation was investigated by whole transcriptome sequencing using the AB SOLiD technology. Among other observations, we noted expression of respiratory complex I NADH-ubiquinone reductase although D. bruxellensis is a Crabtree positive yeast. The observed higher expression of NADH-generating enzymes compared to NAD(+)-generating enzymes might be the reason for the previously observed NADH imbalance and resulting Custer effect in D. bruxellensis. Low expression of genes involved in glycerol production is probably the molecular basis for high efficiency of D. bruxellensis metabolism under nutrient limitation. No D. bruxellensis homologs to the genes involved in the final reactions of glycerol biosynthesis were detected. A high number of expressed sugar transporter genes is consistent with the hypothesis that the competitiveness of D. bruxellensis is due to a higher affinity for the limiting substrate.

  3. Transcriptome of the alternative ethanol production strain Dekkera bruxellensis CBS 11270 in sugar limited, low oxygen cultivation.

    Directory of Open Access Journals (Sweden)

    Ievgeniia A Tiukova

    Full Text Available Dekkera bruxellensis can outcompete Saccharomyces cerevisiae in environments with low sugar concentrations. It is usually regarded as a spoilage yeast but has lately been identified as an alternative ethanol production organism. In this study, global gene expression in the industrial isolate D. bruxellensis CBS 11270 under oxygen and glucose limitation was investigated by whole transcriptome sequencing using the AB SOLiD technology. Among other observations, we noted expression of respiratory complex I NADH-ubiquinone reductase although D. bruxellensis is a Crabtree positive yeast. The observed higher expression of NADH-generating enzymes compared to NAD(+-generating enzymes might be the reason for the previously observed NADH imbalance and resulting Custer effect in D. bruxellensis. Low expression of genes involved in glycerol production is probably the molecular basis for high efficiency of D. bruxellensis metabolism under nutrient limitation. No D. bruxellensis homologs to the genes involved in the final reactions of glycerol biosynthesis were detected. A high number of expressed sugar transporter genes is consistent with the hypothesis that the competitiveness of D. bruxellensis is due to a higher affinity for the limiting substrate.

  4. Cold exposure affects carbohydrates and lipid metabolism, and induces Hog1p phosphorylation in Dekkera bruxellensis strain CBS 2499.

    Science.gov (United States)

    Galafassi, Silvia; Toscano, Marco; Vigentini, Ileana; Zambelli, Paolo; Simonetti, Paolo; Foschino, Roberto; Compagno, Concetta

    2015-05-01

    Dekkera bruxellensis is a yeast known to affect the quality of wine and beer. This species, due to its high ethanol and acid tolerance, has been reported also to compete with Saccharomyces cerevisiae in distilleries producing fuel ethanol. In order to understand how this species responds when exposed to low temperatures, some mechanisms like synthesis and accumulation of intracellular metabolites, changes in lipid composition and activation of the HOG-MAPK pathway were investigated in the genome sequenced strain CBS 2499. We show that cold stress caused intracellular accumulation of glycogen, but did not induce accumulation of trehalose and glycerol. The cellular fatty acid composition changed after the temperature downshift, and a significant increase of palmitoleic acid was observed. RT-PCR analysis revealed that OLE1 encoding for Δ9-fatty acid desaturase was up-regulated, whereas TPS1 and INO1 didn't show changes in their expression. In D. bruxellensis Hog1p was activated by phosphorylation, as described in S. cerevisiae, highlighting a conserved role of the HOG-MAP kinase signaling pathway in cold stress response.

  5. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

    Directory of Open Access Journals (Sweden)

    Tomasz Kulik

    2016-11-01

    Full Text Available Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

  6. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

    Science.gov (United States)

    Kulik, Tomasz; Buśko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; Perkowski, Juliusz; Stenglein, Sebastian

    2016-01-01

    Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium. PMID:27845742

  7. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina.

    Science.gov (United States)

    Kulik, Tomasz; Buśko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D; Perkowski, Juliusz; Stenglein, Sebastian

    2016-11-12

    Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

  8. Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100

    Science.gov (United States)

    Lucas, María; Kortazar, Danel; Astigarraga, Egoitz; Fernández, José A.; Mato, Jose M.; Martínez-Chantar, María Luz; Martínez-Cruz, Luis Alfonso

    2008-01-01

    CBS domains are small protein motifs consisting of a three-stranded β-sheet and two α-helices that are present in proteins of all kingdoms of life and in proteins with completely different functions. Several genetic diseases in humans have been associated with mutations in their sequence, which has made them promising targets for rational drug design. The C-terminal domain of the Methanococcus jannaschii protein MJ0100 includes a CBS-domain pair and has been overexpressed, purified and crystallized. Crystals of selenomethionine-substituted (SeMet) protein were also grown. The space group of both the native and SeMet crystals was determined to be orthorhombic P212121, with unit-cell parameters a = 80.9, b = 119.5, c = 173.3 Å. Preliminary analysis of the X-ray data indicated that there were eight molecules per asymmetric unit in both cases. PMID:18931440

  9. Neuroprotective Roles of l-Cysteine in Attenuating Early Brain Injury and Improving Synaptic Density via the CBS/H2S Pathway Following Subarachnoid Hemorrhage in Rats.

    Science.gov (United States)

    Li, Tong; Wang, Lingxiao; Hu, Quan; Liu, Song; Bai, Xuemei; Xie, Yunkai; Zhang, Tiantian; Bo, Shishi; Gao, Xiangqian; Wu, Shuhua; Li, Gang; Wang, Zhen

    2017-01-01

    l-Cysteine is a semi-essential amino acid and substrate for cystathionine-β-synthase (CBS) in the central nervous system. We previously reported that NaHS, an H2S donor, significantly alleviated brain damage after subarachnoid hemorrhage (SAH) in rats. However, the potential therapeutic value of l-cysteine and the molecular mechanism supporting these beneficial effects have not been determined. This study was designed to investigate whether l-cysteine could attenuate early brain injury following SAH and improve synaptic function by releasing endogenous H2S. Male Wistar rats were subjected to SAH induced by cisterna magna blood injection, and l-cysteine was intracerebroventricularly administered 30 min after SAH induction. Treatment with l-cysteine stimulated CBS activity in the prefrontal cortex (PFC) and H2S production. Moreover, l-cysteine treatment significantly ameliorated brain edema, improved neurobehavioral function, and attenuated neuronal cell death in the PFC; these effects were associated with a decrease in the Bax/Bcl-2 ratio and the suppression of caspase-3 activation 48 h after SAH. Furthermore, l-cysteine treatment activated the CREB-brain-derived neurotrophic factor (BDNF) pathway and intensified synaptic density by regulating synapse proteins 48 h after SAH. Importantly, all the beneficial effects of l-cysteine in SAH were abrogated by amino-oxyacetic acid, a CBS inhibitor. Based on these findings, l-cysteine may play a neuroprotective role in SAH by inhibiting cell apoptosis, upregulating CREB-BDNF expression, and promoting synaptic structure via the CBS/H2S pathway.

  10. Neuroprotective Roles of l-Cysteine in Attenuating Early Brain Injury and Improving Synaptic Density via the CBS/H2S Pathway Following Subarachnoid Hemorrhage in Rats

    Directory of Open Access Journals (Sweden)

    Gang Li

    2017-05-01

    Full Text Available l-Cysteine is a semi-essential amino acid and substrate for cystathionine-β-synthase (CBS in the central nervous system. We previously reported that NaHS, an H2S donor, significantly alleviated brain damage after subarachnoid hemorrhage (SAH in rats. However, the potential therapeutic value of l-cysteine and the molecular mechanism supporting these beneficial effects have not been determined. This study was designed to investigate whether l-cysteine could attenuate early brain injury following SAH and improve synaptic function by releasing endogenous H2S. Male Wistar rats were subjected to SAH induced by cisterna magna blood injection, and l-cysteine was intracerebroventricularly administered 30 min after SAH induction. Treatment with l-cysteine stimulated CBS activity in the prefrontal cortex (PFC and H2S production. Moreover, l-cysteine treatment significantly ameliorated brain edema, improved neurobehavioral function, and attenuated neuronal cell death in the PFC; these effects were associated with a decrease in the Bax/Bcl-2 ratio and the suppression of caspase-3 activation 48 h after SAH. Furthermore, l-cysteine treatment activated the CREB–brain-derived neurotrophic factor (BDNF pathway and intensified synaptic density by regulating synapse proteins 48 h after SAH. Importantly, all the beneficial effects of l-cysteine in SAH were abrogated by amino-oxyacetic acid, a CBS inhibitor. Based on these findings, l-cysteine may play a neuroprotective role in SAH by inhibiting cell apoptosis, upregulating CREB–BDNF expression, and promoting synaptic structure via the CBS/H2S pathway.

  11. Assessment of CCSD(T), MP2, DFT-D, CBS-QB3, and G4(MP2) methods for conformational study of alanine and proline dipeptides

    Science.gov (United States)

    Kang, Young Kee; Park, Hae Sook

    2014-04-01

    The CCSD(T), MP2, dispersion-corrected DFT, CBS-QB3, and G4(MP2) levels of theory with various basis sets are assessed for their ability to describe the conformational preferences of the Ala and Pro dipeptides. The ωB97X-D/6-311++G(d,p) level of theory provided the rotational constants of the most stable conformer of the Ala dipeptide consistent with the values from microwave experiments. The double-hybrid DSD-PBEP86-D3BJ DFT method provided the best performance for relative energies of both dipeptides consistent with CCSD(T)/CBS limit values. The DSD-PBEP86-D3BJ/cc-pVTZ//M06-2X/6-31+G(d), B2PLYPD-D3BJ/cc-pVTZ//M06-2X/6-31+G(d), and M06-2X/cc-pVTZ//M06-2X/6-31+G(d) levels of theory may be an alternative to the CCSD(T)/CBS limit//ωB97X-D/6-311++G(d,p) level of theory with marginal deviations for conformational study of peptides.

  12. Sex-specific association of sequence variants in CBS and MTRR with risk for promoter hypermethylation in the lung epithelium of smokers.

    Science.gov (United States)

    Flores, Kristina G; Stidley, Christine A; Mackey, Amanda J; Picchi, Maria A; Stabler, Sally P; Siegfried, Jill M; Byers, Tim; Berwick, Marianne; Belinsky, Steven A; Leng, Shuguang

    2012-08-01

    Gene promoter hypermethylation is now regarded as a promising biomarker for the risk and progression of lung cancer. The one-carbon metabolism pathway is postulated to affect deoxyribonucleic acid (DNA) methylation because it is responsible for the generation of S-adenosylmethionine (SAM), the methyl donor for cellular methylation reactions. This study investigated the association of single nucleotide polymorphisms (SNPs) in six one-carbon metabolism-related genes with promoter hypermethylation in sputum DNA from non-Hispanic white smokers in the Lovelace Smokers Cohort (LSC) (n = 907). Logistic regression was used to assess the association of SNPs with hypermethylation using a high/low methylation cutoff. SNPs in the cystathionine beta synthase (CBS) and 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR) genes were significantly associated with high methylation in males [CBS rs2850146 (-8283G > C), OR = 4.9; 95% CI: 1.98, 12.2, P = 0.0006] and low methylation in females [MTRR rs3776467 (7068A > G), OR = 0.57, 95% CI: 0.42, 0.77, P = 0.0003]. The variant allele of rs2850146 was associated with reduced gene expression and increased plasma homocysteine (Hcy) concentrations. Three plasma metabolites, Hcy, methionine and dimethylglycine, were associated with increased risk for gene methylation. These studies suggest that SNPs in CBS and MTRR have sex-specific associations with aberrant methylation in the lung epithelium of smokers that could be mediated by the affected one-carbon metabolism and transsulfuration in the cells.

  13. [Serum cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE ) and cytochrome c oxidase (COX) in copper mine miners potentially expose d to hydrogen sulfide].

    Science.gov (United States)

    Skoczyńska, Anna; Gruszczyński, Leszek; Turczyn, Barbara; Ścieszka, Marek; Wojakowska, Anna; Pawłowski, Tomasz; Schmidt, Edward

    2015-01-01

    The aim of the study was to evaluate serum levels of the target enzyme for H2S toxicity--cytochrome c oxidase (COX) and enzymes involved in the synthesis of H2S--cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) in copper mine miners. The initial and basic study was conducted respectively in 237 and 88 miners, working in 2 mining shafts: I--no H2S emissions recorded in the last 10 years (study group A) and II--H2S emissions occurred (study group B). A medical examination was performed and 10 ml of blood was collected from miners immediately after exiting the mine. There were no clinical or biochemical changes typical for H2S toxicity. Sulfhemoglobine was undetectable and there were no changes in the red-ox system. However, in group B, regulatory changes were found; a tendency to higher concentration of CBS and CSE, a higher activity of angiotensin converting enzyme (ACE) compared to group A (pCBS and CSE activity. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.

  14. rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment.

    Science.gov (United States)

    Russo, Annapina; Saide, Assunta; Cagliani, Roberta; Cantile, Monica; Botti, Gerardo; Russo, Giulia

    2016-12-07

    5-FU is a chemotherapy drug commonly used for the treatment of human cancers; however drug resistance represents a major challenge for its clinical application. In the present study, we reporte that rpL3 induced by 5-FU treatment in Calu-6 cells represses CBS transcription and reduces CBS protein stability leading to a decrease of CBS protein levels. rpL3 also regulates negatively the activation of NFκB by preventing NFκB nuclear translocation through IκB-α up-regulation. Furthermore, we demonstrate that rpL3 significantly enhances the apoptosis of 5-FU treated Calu-6 cells promoting the overexpression of the pro-apoptotic proteins Bax and the inhibition of the anti-apoptotic protein Bcl-2. We finally demonstrate that rpL3 potentiates 5-FU efficacy inhibiting cell migration and invasion. Our results suggest that combination of rpL3 and 5-FU is a promising strategy for chemotherapy of lung cancers lacking functional p53 that are resistant to 5-FU.

  15. Urothelium muscarinic activation phosphorylates CBS(Ser227) via cGMP/PKG pathway causing human bladder relaxation through H2S production.

    Science.gov (United States)

    d'Emmanuele di Villa Bianca, Roberta; Mitidieri, Emma; Fusco, Ferdinando; Russo, Annapina; Pagliara, Valentina; Tramontano, Teresa; Donnarumma, Erminia; Mirone, Vincenzo; Cirino, Giuseppe; Russo, Giulia; Sorrentino, Raffaella

    2016-08-11

    The urothelium modulates detrusor activity through releasing factors whose nature has not been clearly defined. Here we have investigated the involvement of H2S as possible mediator released downstream following muscarinic (M) activation, by using human bladder and urothelial T24 cell line. Carbachol stimulation enhances H2S production and in turn cGMP in human urothelium or in T24 cells. This effect is reversed by cysthationine-β-synthase (CBS) inhibition. The blockade of M1 and M3 receptors reverses the increase in H2S production in human urothelium. In T24 cells, the blockade of M1 receptor significantly reduces carbachol-induced H2S production. In the functional studies, the urothelium removal from human bladder strips leads to an increase in carbachol-induced contraction that is mimicked by CBS inhibition. Instead, the CSE blockade does not significantly affect carbachol-induced contraction. The increase in H2S production and in turn of cGMP is driven by CBS-cGMP/PKG-dependent phosphorylation at Ser(227) following carbachol stimulation. The finding of the presence of this crosstalk between the cGMP/PKG and H2S pathway downstream to the M1/M3 receptor in the human urothelium further implies a key role for H2S in bladder physiopathology. Thus, the modulation of the H2S pathway can represent a feasible therapeutic target to develop drugs for bladder disorders.

  16. CBS方法对单杂环体系C—H键解离焓的实验值评估%Assessment on Experimental Values of C—H Bond Dissociation Enthalpies of Single Heterocyclic Molecular with CBS Methods

    Institute of Scientific and Technical Information of China (English)

    吴晨波; 王颖星; 郑文锐

    2014-01-01

    用高精度计算方法CBS-4M,CBS-Q和CBS-QB3评估了26种单杂环分子的C—H键解离焓的实验值.结果表明,CBS-QB3方法最好,MD、MAD及RMSE分别为9.3、9.3和11.1 kJ/mol.理论值与实验值的线性相关方程为y=0.928x+ 21.3,相关系数平方R2达0.980.通过自然键轨道分析后发现,杂原子的电负性使得五元杂环的自然电荷分布发生变化.

  17. Model selection for global and diffuse radiation over the Central Black Sea (CBS) region of Turkey

    Energy Technology Data Exchange (ETDEWEB)

    Tarhan, Sefa [Gaziosmanpasa Univ., Dept. of Farm Machinery, Tokat (Turkey); Sari, Ahmet [Gaziosmanpasa Univ., Dept. of Chemistry, Tokat (Turkey)

    2005-03-01

    Solar radiation over the Central Black Sea Region of Turkey, covering the five provinces (Amasya, Corum, Ordu, Samsun and Tokat), was analyzed to guide future projects. The maximum monthly average global solar radiation ranged from 20.05 (for Ordu) to 23.71 MJ m{sup -2} (for Tokat). The maximum monthly average daily measured sunshine duration ranged from 6.89 (for Ordu) to 11 h (for Corum). A quadratic polynomial equation was empirically developed to predict the monthly average daily global radiation. A hybrid model was also developed based on the predictions of six existing models to predict the monthly average daily diffuse and beam radiation. Various statistical tests (analysis of variance for model adequacy and t-test for significance of model parameter etc.) have shown that the models developed in this study are adequate for solar radiation prediction. (Author)

  18. On the nature of stabilization in weak, medium, and strong charge-transfer complexes: CCSD(T)/CBS and SAPT calculations.

    Science.gov (United States)

    Karthikeyan, S; Sedlak, Robert; Hobza, Pavel

    2011-09-01

    Weak, medium, and strong charge-transfer (CT) complexes containing various electron donors (C(2)H(4), C(2)H(2), NH(3), NMe(3), HCN, H(2)O) and acceptors (F(2), Cl(2), BH(3), SO(2)) were investigated at the CCSD(T)/complete basis set (CBS) limit. The nature of the stabilization for these CT complexes was evaluated on the basis of perturbative NBO calculations and DFT-SAPT/CBS calculations. The structure of all of the complexes was determined by the counterpoise-corrected gradient optimization performed at the MP2/cc-pVTZ level, and most of complexes possess a linear-like contact structure. The total stabilization energies lie between 1 and 55 kcal/mol and the strongest complexes contain BH(3) as an electron acceptor. When ordering the electron donors and electron acceptors on the basis of these energies, we obtain the same order as that based on the perturbative E2 charge-transfer energies, which provides evidence that the charge-transfer term is the dominant energy contribution. The CCSD(T) correction term, defined as the difference between the CCSD(T) and MP2 interaction energies, is mostly small, which allows the investigation of the CT complexes of this type at the "cheap" MP2/CBS level. In the case of weak and medium CT complexes (with stabilization energy smaller than about 15 kcal/mol), the dominant stabilization originates in the electrostatic term; the dispersion as well as induction and δ(HF) terms covering the CT energy contribution are, however, important as well. For strong CT complexes, induction energy is the second (after electrostatic) most important energy term. The role of the induction and δ(HF) terms is unique and characteristic for CT complexes. For all CT complexes, the CCSD(T)/CBS and DFT-SAPT/CBS stabilization energies are comparable, and surprisingly, it is true even for very strong CT complexes with stabilization energy close to 50 kcal/mol characteristic by substantial charge transfer (more than 0.3 e). It is thus possible to conclude

  19. Molecular dynamics simulations of fluid cyclopropane with MP2/CBS-fitted intermolecular interaction potentials.

    Science.gov (United States)

    Ho, Yen-Ching; Wang, Yi-Siang; Chao, Sheng D

    2017-08-14

    Modeling fluid cycloalkanes with molecular dynamics simulations has proven to be a very challenging task partly because of lacking a reliable force field based on quantum chemistry calculations. In this paper, we construct an ab initio force field for fluid cyclopropane using the second-order Møller-Plesset perturbation theory. We consider 15 conformers of the cyclopropane dimer for the orientation sampling. Single-point energies at important geometries are calibrated by the coupled cluster with single, double, and perturbative triple excitation method. Dunning's correlation consistent basis sets (up to aug-cc-pVTZ) are used in extrapolating the interaction energies at the complete basis set limit. The force field parameters in a 9-site Lennard-Jones model are regressed by the calculated interaction energies without using empirical data. With this ab initio force field, we perform molecular dynamics simulations of fluid cyclopropane and calculate both the structural and dynamical properties. We compare the simulation results with those using an empirical force field and obtain a quantitative agreement for the detailed atom-wise radial distribution functions. The experimentally observed gross radial distribution function (extracted from the neutron scattering measurements) is well reproduced in our simulation. Moreover, the calculated self-diffusion coefficients and shear viscosities are in good agreement with the experimental data over a wide range of thermodynamic conditions. To the best of our knowledge, this is the first ab initio force field which is capable of competing with empirical force fields for simulating fluid cyclopropane.

  20. Molecular dynamics simulations of fluid cyclopropane with MP2/CBS-fitted intermolecular interaction potentials

    Science.gov (United States)

    Ho, Yen-Ching; Wang, Yi-Siang; Chao, Sheng D.

    2017-08-01

    Modeling fluid cycloalkanes with molecular dynamics simulations has proven to be a very challenging task partly because of lacking a reliable force field based on quantum chemistry calculations. In this paper, we construct an ab initio force field for fluid cyclopropane using the second-order Møller-Plesset perturbation theory. We consider 15 conformers of the cyclopropane dimer for the orientation sampling. Single-point energies at important geometries are calibrated by the coupled cluster with single, double, and perturbative triple excitation method. Dunning's correlation consistent basis sets (up to aug-cc-pVTZ) are used in extrapolating the interaction energies at the complete basis set limit. The force field parameters in a 9-site Lennard-Jones model are regressed by the calculated interaction energies without using empirical data. With this ab initio force field, we perform molecular dynamics simulations of fluid cyclopropane and calculate both the structural and dynamical properties. We compare the simulation results with those using an empirical force field and obtain a quantitative agreement for the detailed atom-wise radial distribution functions. The experimentally observed gross radial distribution function (extracted from the neutron scattering measurements) is well reproduced in our simulation. Moreover, the calculated self-diffusion coefficients and shear viscosities are in good agreement with the experimental data over a wide range of thermodynamic conditions. To the best of our knowledge, this is the first ab initio force field which is capable of competing with empirical force fields for simulating fluid cyclopropane.

  1. Theoretical reduction potentials for nitrogen oxides from CBS-QB3 energetics and (C)PCM solvation calculations.

    Science.gov (United States)

    Dutton, Andrew S; Fukuto, Jon M; Houk, Kendall N

    2005-05-30

    The complete basis set method, CBS-QB3, is used in combination with two continuum solvation models for aqueous solvation to compute reduction potentials previously determined experimentally for 36 nitrogen oxides and related species of the general formula H(V)C(W)N(X)O(Y)Cl(Z). The PCM model led to the correlation E(o)exp (vs NHE) = 0.84E(o)calc + 0.03 V with an average error of 0.12 V (2.8 kcal/mol) and a maximum error of 0.32 V (7.4 kcal/mol). The CPCM/UAKS model gave E(o)exp (vs NHE) = 0.83E(o)calc + 0.11 V with the same average error. This general method was used to predict reduction potentials (+/-0.3 V) for nitrogen oxides for which reduction potentials are not known with certainty: NO2/NO2- (0.6 V), NO3/NO3- (1.9 V), N2O3-/N2O3(2-) (0.5 V), HN2O3/HN2O3- (0.9 V), HONNO,H+/HONNOH (1.6 V), 2NO,H+/HONNO (0.0 V), 2NO/ONNO- (-0.1 V), ONNO-/ONNO(2-) (-0.4 V), HNO,H+/H2NO (0.6 V), H2NO,H+/H2NOH (0.9 V), HNO,2H+/H2NOH (0.8 V), and HNO/HNO- (-0.7 V).

  2. Head Space Solid Phase Micro-Extraction (HS - SPME of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636

    Directory of Open Access Journals (Sweden)

    Eunice Valduga

    2010-12-01

    Full Text Available The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636 using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME. Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm, temperature (25-60 ºC, extraction time (10-30 minutes, and sample volume (2-3 mL. The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD. The conditions that resulted in maximum extraction were: 60 ºC, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v. In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm, temperature (23-33 ºC, pH (4.0-8.0, precursor concentration (0.02-0.1%, mannitol (0-6%, and asparagine concentration (0-0.2% was investigated. The bio-production at 28 ºC, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.

  3. Study on Environmental Causes and SNPs of MTHFR, MS and CBS Genes Related to Congenital Heart Disease.

    Directory of Open Access Journals (Sweden)

    Hui Shi

    Full Text Available Congenital heart diseases (CHD are among the most common birth defects in China. Environmental causes and folate metabolism changes may alter susceptibility to CHD. The aim of this study is to evaluate the relevant risk-factors of children with CHD and their mothers.138 children with CHD and 207 normal children for controls were recruited. Their mothers were also enlisted in this study and interviewed following a questionnaire about their pregnant history and early pregnancy situation. Five single nucleotide polymorphisms (SNPs in methylenetetrahydrofolate reductase (MTHFR, methionine synthase (MS and cystathionine β-synthase (CBS of mothers and children were genotyped.There were significant differences in the gender of children, occupation of mothers, family history with CHD, history of abortion, history of adverse pregnancy, early pregnancy health, fetus during pregnancy, pesticide exposure and drug exposure in CHD group and control group ( P < 0.05. Logistic regression analyses showed that after adjustment for above factors, MTHFR rs1801131 were significantly associated with their offspring CHD risk in mothers. Compared with the mothers whose MTHFR were rs1801131 AA and AC genotypes, the mothers who got a mutation of MTHFR rs1801131 CC genotypes had a 267% increase in risk of given birth of a CHD children (OR = 3.67,95%CI = 1.12-12.05. Meanwhile, MTHFR rs1801131 were significantly associated with CHD susceptibility in children (OR = 1.42, 95% CI = 1.00-2.44 in additive model.Besides mothers' social and fertility characteristics, our results suggested that the genetic variants in folate metabolism pathway might be one of the most related risk-factors of CHD. MTHFR rs1801131 were identified as loci in Chinese population that were involved in CHD.

  4. Study on Environmental Causes and SNPs of MTHFR, MS and CBS Genes Related to Congenital Heart Disease.

    Science.gov (United States)

    Shi, Hui; Yang, Shiwei; Liu, Yan; Huang, Peng; Lin, Ning; Sun, Xiaoru; Yu, Rongbin; Zhang, Yuanyuan; Qin, Yuming; Wang, Lijuan

    2015-01-01

    Congenital heart diseases (CHD) are among the most common birth defects in China. Environmental causes and folate metabolism changes may alter susceptibility to CHD. The aim of this study is to evaluate the relevant risk-factors of children with CHD and their mothers. 138 children with CHD and 207 normal children for controls were recruited. Their mothers were also enlisted in this study and interviewed following a questionnaire about their pregnant history and early pregnancy situation. Five single nucleotide polymorphisms (SNPs) in methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MS) and cystathionine β-synthase (CBS) of mothers and children were genotyped. There were significant differences in the gender of children, occupation of mothers, family history with CHD, history of abortion, history of adverse pregnancy, early pregnancy health, fetus during pregnancy, pesticide exposure and drug exposure in CHD group and control group ( P < 0.05). Logistic regression analyses showed that after adjustment for above factors, MTHFR rs1801131 were significantly associated with their offspring CHD risk in mothers. Compared with the mothers whose MTHFR were rs1801131 AA and AC genotypes, the mothers who got a mutation of MTHFR rs1801131 CC genotypes had a 267% increase in risk of given birth of a CHD children (OR = 3.67,95%CI = 1.12-12.05). Meanwhile, MTHFR rs1801131 were significantly associated with CHD susceptibility in children (OR = 1.42, 95% CI = 1.00-2.44 in additive model). Besides mothers' social and fertility characteristics, our results suggested that the genetic variants in folate metabolism pathway might be one of the most related risk-factors of CHD. MTHFR rs1801131 were identified as loci in Chinese population that were involved in CHD.

  5. Chemical Composition and Medicinal Value of the New Ganoderma tsugae var. jannieae CBS-120304 Medicinal Higher Basidiomycete Mushroom.

    Science.gov (United States)

    Chan, Jannie Siew Lee; Asatiani, Mikheil D; Sharvit, Lital E; Trabelcy, Beny; Barseghyan, Gayane S; Wasser, Solomon P

    2015-01-01

    In this research, the chemical composition and anticancer and antioxidant activity of the new medicinal mushroom Ganoderma tsugae var. jannieae CBS-120304 were evaluated. The chemical composition assay includes amounts of total carbohydrates and proteins, amino acids, fatty acids, micro- and macroelements, and vitamins. The investigated medicinal mushroom seemed to be a rich source of nutritional components. Mycelium accumulated more than 2-fold more total protein compared with the fruiting body and reached 37% and 16% of dry weight, respectively. Carbohydrate content in the fruiting body seemed to be conspicuously higher than in the mycelium (50% of dry weight) and reached 80% of dry weight. Quantification of the identified fatty acids indicated that, in general, palmitic acid, oleic acid, and linoleic acid were the major fatty acids. Toxic elements, such as silver, arsenic, cadmium, and mercury, were found only in trace amounts in mycelium and were not detected in the fruiting body. Furthermore, the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay was used to evaluate antioxidant activity. The highest radical scavenging activity was 9.0 mg/mL (65.9%) by ethanol extract. In addition, mycelial extracts were tested to inhibit MCF7 breast cancer cells. Ganoderma tsugae var. jannieae ethyl acetate extract (GTEAE) extract showed high potential by inhibiting reporter activity by more than 70%. Results demonstrated that GTEAE had a strong effect on inhibitory protein κΒα level in the higher concentration used (200 gg/mL), which could be compared with the effect of parthenolide. Furthermore, GTEAE demonstrated strong inhibition of IκΒα phosphorylation.

  6. Reference MP2/CBS and CCSD(T) quantum-chemical calculations on stacked adenine dimers. Comparison with DFT-D, MP2.5, SCS(MI)-MP2, M06-2X, CBS(SCS-D) and force field descriptions.

    Science.gov (United States)

    Morgado, Claudio A; Jurecka, Petr; Svozil, Daniel; Hobza, Pavel; Sponer, Jirí

    2010-04-14

    We have performed reference quantum-chemical calculations for about 130 structures of adenine dimers in stacked conformations, with special attention given to dimers that are either vertically compressed (parallel structures) or contain close interatomic contacts (non-parallel structures). Such geometries are sampled during thermal fluctuations of nucleic acids and contribute to the local conformational variability of these systems. Their theoretical characterization requires a good description of interaction energies in the short-range repulsion region. The reference calculations have been performed with the CBS(T) method, i.e., MP2/CBS computations corrected for higher-order electron-correlation effects using the CCSD(T) method. These benchmark data have been used to examine the performance of the DFT-D, SCS(MI)-MP2, MP2.5, M06-2X and CBS(SCS-D) quantum-mechanical methods, and of the AMBER Cornell et al. force field. The present results, as well as those of our previous study on stacked uracil dimers, confirm that the force field severely exaggerates the repulsion at short intermolecular distances. This behavior complicates the use of the force field in scans of the stacking-energy dependence on local conformational parameters in nucleic acids. Compared against the previous results obtained in the uracil dimer study, the performance of DFT-D to describe stacking at short intermolecular distances has worsened, showing for the adenine dimers a larger exaggeration of the repulsion, especially for structures where the monomers are parallel to each other. Despite these deviations, the performance of DFT-D is still reasonably good and this method provides, for example, a relatively inexpensive way to monitor stacking energies along molecular dynamics trajectories. The best performers are the MP2.5, SCS(MI)-MP2, and CBS(SCS-D) methods. In addition, the energy profiles given by the SCS(MI)-MP2 and CBS(SCS-D) methods are the ones that most closely resemble the CBS(T) data

  7. Nucleotide binding triggers a conformational change of the CBS module of the magnesium transporter CNNM2 from a twisted towards a flat structure.

    Science.gov (United States)

    Corral-Rodríguez, María Ángeles; Stuiver, Marchel; Abascal-Palacios, Guillermo; Diercks, Tammo; Oyenarte, Iker; Ereño-Orbea, June; de Opakua, Alain Ibáñez; Blanco, Francisco J; Encinar, José Antonio; Spiwok, Vojtêch; Terashima, Hiroyuki; Accardi, Alessio; Müller, Dominik; Martínez-Cruz, Luis Alfonso

    2014-11-15

    Recent studies suggest CNNM2 (cyclin M2) to be part of the long-sought basolateral Mg2+ extruder at the renal distal convoluted tubule, or its regulator. In the present study, we explore structural features and ligand-binding capacities of the Bateman module of CNNM2 (residues 429-584), an intracellular domain structurally equivalent to the region involved in Mg2+ handling by the bacterial Mg2+ transporter MgtE, and AMP binding by the Mg2+ efflux protein CorC. Additionally, we studied the structural impact of the pathogenic mutation T568I located in this region. Our crystal structures reveal that nucleotides such as AMP, ADP or ATP bind at only one of the two cavities present in CNNM2429-584. Mg2+ favours ATP binding by alleviating the otherwise negative charge repulsion existing between acidic residues and the polyphosphate group of ATP. In crystals CNNM2429-584 forms parallel dimers, commonly referred to as CBS (cystathionine β-synthase) modules. Interestingly, nucleotide binding triggers a conformational change in the CBS module from a twisted towards a flat disc-like structure that mostly affects the structural elements connecting the Bateman module with the transmembrane region. We furthermore show that the T568I mutation, which causes dominant hypomagnesaemia, mimics the structural effect induced by nucleotide binding. The results of the present study suggest that the T568I mutation exerts its pathogenic effect in humans by constraining the conformational equilibrium of the CBS module of CNNM2, which becomes 'locked' in its flat form.

  8. Applying the health belief model to examine news coverage regarding steroids in sports by ABC, CBS, and NBC between March 1990 and May 2008.

    Science.gov (United States)

    Quick, Brian L

    2010-04-01

    The investigation described here examined ABC, CBS, and NBC news coverage of steroids in sports between March 1990 and May 2008. Employing a framing analysis guided by the health belief model (HBM), coverage of the barriers and benefits of using steroids is reported. Overall, the trend by these three news affiliates was to emphasize the illegality of using steroids, whereas considerably less coverage was devoted to the health costs, in terms of both severity and susceptibility, of using steroids. Furthermore, of the health costs reported, the specific consequences of steroid use varied considerably. The results are reported across four timeframes: 1990-2008, 1990-1996, 1997-2002, and 2003-2008.

  9. Cloning and characterization of an endo-b-1,3(4) glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938

    DEFF Research Database (Denmark)

    Villadsen, Ingrid; Bang, M_L; Sandal, T.

    1999-01-01

    We describe the identification and expression cloning of two novel enzymes, a P-glucanase and an aspartic protease, secreted from the basidiomycetous yeast Phaffia rhodozyma. A cDNA library from P. rhodozyma CBS 6938 was constructed, and full-length cDNA encoding an endo-1,3(4)-beta-glucanase (bg1......) and an aspartic protease (pr1) were cloned by expression cloning in Saccharomyces cerevisiae W3124. The bgl cDNA encodes a 424-residue precursor protein with a putative signal peptide. The prl cDNA encodes a 405-residue prepropolypeptide with an 81-residue leader peptide. The aspartic protease was purified...

  10. Effects of B{sub 2}O{sub 3} content and sintering temperature on crystallization and microstructure of CBS glass–ceramic coatings

    Energy Technology Data Exchange (ETDEWEB)

    Li, Pengyang [School of Materials Science and Engineering, Beihang University, Beijing 100191 (China); Wang, Shubin, E-mail: shubinwang@buaa.edu.cn [School of Materials Science and Engineering, Beihang University, Beijing 100191 (China); Key Laboratory of Aerospace Materials and Performance (Ministry of Education), School of Materials and Engneering, Beihang University, Beijing 100191 (China); Liu, Jianggao; Feng, Mengjie; Yang, Xinwang [School of Materials Science and Engineering, Beihang University, Beijing 100191 (China)

    2015-11-30

    Graphical abstract: (a) TEM photogram of CG3 sintered at 800 °C, crystals are obvious; (b) the XRD patterns of CG3 glass samples sintered at various temperatures; (c) SEM photogram of CG3 sintered at 800 °C; (d) Kissinger, Augis–Bennett and Ozawa kinetics plots of CG3 glass samples. - Highlights: • Combining sol–gel method with direct sintering method to reduce the temperature of coatings formation. • Characterizing CaO–SiO{sub 2}–B{sub 2}O{sub 3} glass–ceramic coatings on porous substrates. • Surface crystallization of CBS glass–ceramic coatings: nucleation and kinetics. • Activation energies for crystal growth in CBS glass–ceramics with different contents of B{sub 2}O{sub 3}. - Abstract: Borosilicate glass–ceramics precursors with varying compositional ratios in the CaO–SiO{sub 2}–B{sub 2}O{sub 3} (CBS) system were synthesized by sol–gel method. The precursors were calcined at 1200 °C for 2 h to form glass powders. The glass–ceramics were prepared by overlaying glass slurries on the substrates before sintering at different temperatures. The as-prepared glasses and glass–ceramics were characterized by differential scanning calorimetry and X-ray diffraction. The crystallization activation energies (E{sub c}) were calculated using the Kissinger method from DSC results. The morphology and crystallization behavior of the glass–ceramics were monitored by scanning electron microscopy. Both glass transition and crystallization temperatures decreased, however, the metastable zone increased. The E{sub c} values of CBS glasses and glass–ceramics were 254.1, 173.2 and 164.4 kJ/mol with increasing B{sub 2}O{sub 3} content, whereas that of the calcined G3 glass was 104.9 kJ/mol. Finally, the coatings were prepared at a low temperature (700 °C). The crystals that grew on the surface of multilayer coatings demonstrated heterogeneous surface nucleation and crystallization after heat-treatment from 700 °C to 850 °C for 4 h.

  11. Genome sequence of the type strain CLIB 1764T (=CBS 14374T of the yeast species Kazachstania saulgeensis isolated from French organic sourdough

    Directory of Open Access Journals (Sweden)

    Véronique Sarilar

    2017-09-01

    Full Text Available Kazachstania saulgeensis is a recently described species isolated from French organic sourdough. Here, we report the high quality genome sequence of a monosporic segregant of the type strain of this species, CLIB 1764T (=CBS 14374T. The genome has a total length of 12.9 Mb and contains 5326 putative protein-coding genes, excluding pseudogenes and transposons. The nucleotide sequences were deposited into the European Nucleotide Archive under the genome assembly accession numbers FXLY01000001–FXLY01000017.

  12. Genome sequence of the type strain CLIB 1764(T) (= CBS 14374(T)) of the yeast species Kazachstania saulgeensis isolated from French organic sourdough.

    Science.gov (United States)

    Sarilar, Véronique; Sterck, Lieven; Matsumoto, Saki; Jacques, Noémie; Neuvéglise, Cécile; Tinsley, Colin R; Sicard, Delphine; Casaregola, Serge

    2017-09-01

    Kazachstania saulgeensis is a recently described species isolated from French organic sourdough. Here, we report the high quality genome sequence of a monosporic segregant of the type strain of this species, CLIB 1764(T) (= CBS 14374(T)). The genome has a total length of 12.9 Mb and contains 5326 putative protein-coding genes, excluding pseudogenes and transposons. The nucleotide sequences were deposited into the European Nucleotide Archive under the genome assembly accession numbers FXLY01000001-FXLY01000017.

  13. Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

    OpenAIRE

    Ilmi, Miftahul; Hidayat, C.; Hastuti, P .; Heeres, Hero; Maarel, Marc van der

    2017-01-01

    Jatropha curcas L. is a tropical plant that grows well on poor soils in arid regions and produces oil-rich, unedible seeds. The oil from these seeds can be used in the production of biodiesel. In addition, the press-cake waste that is left after oil extraction contains approximately 40% of protein. The use of Jatropha press-cake as substrate for fungal growth and the production of lipase by Aspergillus niger 65I6 isolated from Indonesia and Rhizomucor miehei CBS 260.62 was investigated. Hydro...

  14. Binding of S-methyl-5'-thioadenosine and S-adenosyl-L-methionine to protein MJ0100 triggers an open-to-closed conformational change in its CBS motif pair.

    Science.gov (United States)

    Lucas, María; Encinar, José Antonio; Arribas, Egoitz Astigarraga; Oyenarte, Iker; García, Inmaculada Gómez; Kortazar, Danel; Fernández, José A; Mato, Jose M; Martínez-Chantar, María Luz; Martínez-Cruz, Luis Alfonso

    2010-02-26

    Cystathionine beta-synthase (CBS) domains are small motifs that are present in proteins with completely different functions. Several genetic diseases in humans have been associated with mutations in their sequence, which has made them promising targets for rational drug design. The protein MJ0100 from Methanocaldococcus jannaschii includes a DUF39 domain of so far unknown function and a CBS domain pair (Bateman domain) at its C-terminus. This work presents the crystallographic analysis of four different states of the CBS motif pair of MJ0100 in complex with different numbers of S-adenosyl-L-methionine (SAM) and S-methyl-5'-thioadenosine (MTA) ligands, providing evidence that ligand-induced conformational reorganization of Bateman domain dimers could be an important regulatory mechanism. These observations are in contrast to what is known from most of the other Bateman domain structures but are supported by recent studies on the magnesium transporter MgtE. Our structures represent the first example of a CBS domain protein complexed with SAM and/or MTA and might provide a structural basis for understanding the molecular mechanisms regulated by SAM upon binding to the C-terminal domain of human CBS, whose structure remains unknown. Copyright (c) 2009. Elsevier Ltd. All rights reserved.

  15. Breaking the bottleneck: Use of molecular tailoring approach for the estimation of binding energies at MP2/CBS limit for large water clusters

    Science.gov (United States)

    Singh, Gurmeet; Nandi, Apurba; Gadre, Shridhar R.

    2016-03-01

    A pragmatic method based on the molecular tailoring approach (MTA) for estimating the complete basis set (CBS) limit at Møller-Plesset second order perturbation (MP2) theory accurately for large molecular clusters with limited computational resources is developed. It is applied to water clusters, (H2O)n (n = 7, 8, 10, 16, 17, and 25) optimized employing aug-cc-pVDZ (aVDZ) basis-set. Binding energies (BEs) of these clusters are estimated at the MP2/aug-cc-pVNZ (aVNZ) [N = T, Q, and 5 (whenever possible)] levels of theory employing grafted MTA (GMTA) methodology and are found to lie within 0.2 kcal/mol of the corresponding full calculation MP2 BE, wherever available. The results are extrapolated to CBS limit using a three point formula. The GMTA-MP2 calculations are feasible on off-the-shelf hardware and show around 50%-65% saving of computational time. The methodology has a potential for application to molecular clusters containing ˜100 atoms.

  16. Detection of the gene encoding the small subunit of the CO dehydrogenase enzyme in the H{sub 2}-evolving bacterium Rubrivivax gelatinosus CBS

    Energy Technology Data Exchange (ETDEWEB)

    Kish, A.; Levin, D. [Victoria Univ., BC (Canada)]|[Victoria Univ., BC (Canada)

    2001-06-01

    A purple non-sulfur bacterium, Rubrivivax gelatinosus CBS presents great opportunities, on a commercial scale, for the biological hydrogen production. A water-gas shift reaction is catalyzed when the bacterium is cultured in the presence of carbon oxide in the dark. The result is carbon monoxide (and water) being shifted into hydrogen (H{sub 2}) and carbon dioxide in near stoichiometric quantities. The production of hydrogen as a clean alternative fuel could be accomplished by using carbon monoxide generated from gasified waste biomass, using the bacterial water-gas shift reaction for that purpose. The characterization of three key enzymes and the genes encoding them was performed in a closely related purple non-sulfur bacterium called Rhodospirillum rubrum. They were: (1) a carbon monoxide dehydrogenase (CODH), (2) the ferredoxin-like electron-carrier small subunit of the CODH enzyme, and (3) an hydrogen-evolving hydrogenase. A transcriptional unit separate from the genes encoding the CODH and its ferredoxin-like small subunit encode the genes for the hydrogenase. A fragment of the Rhodospirillum rubrum ferredoxin-like subunit gene was amplified through the use of a polymerase chain reaction. Southern blots of restriction endonuclease digested genomic deoxyribonucleic acid (DNA) extracted from Rubrivivax gelatinosus CBS was probed with the fragment of the Rhodospirillum rubrum previously amplified using the polymerase chain reaction. Confirmation of the identification is being confirmed, while the gene is sequenced. 25 refs., 2 figs.

  17. Efficacy and gastrointestinal tolerability of ML3403, a selective inhibitor of p38 MAP kinase and CBS-3595, a dual inhibitor of p38 MAP kinase and phosphodiesterase 4 in CFA-induced arthritis in rats.

    Science.gov (United States)

    Koch, Diana A; Silva, Rodrigo B M; de Souza, Alessandra H; Leite, Carlos E; Nicoletti, Natália F; Campos, Maria M; Laufer, Stefan; Morrone, Fernanda B

    2014-03-01

    Mitogen-activated protein kinase (MAPK) p38 inhibitors have entered the clinical phase, although many of them have failed due to high toxicity and lack of efficacy. In the present study we compared the effects of the selective p38 inhibitor ML3403 and the dual p38-PDE4 inhibitor CBS-3595, on inflammatory and nociceptive parameters in a model of polyarthritis in rats. Male Wistar rats (180-200 g) were used for the complete Freund's adjuvant (CFA)-induced arthritis model and they were evaluated at 14-21 days. We also analysed the effects of these pharmacological tools on liver and gastrointestinal toxicity and on cytokine levels. Repeated CBS-3595 (3 mg/kg) or ML3403 (10 mg/kg) administration produced significant anti-inflammatory actions in the chronic arthritis model induced by CFA. CBS-3595 and ML3403 treatment also markedly reduced the production of the proinflammatory cytokine IL-6 in the paw tissue, whereas it widely increased the levels of the anti-inflammatory cytokine IL-10. Moreover, CBS-3595 produced partial anti-allodynic effects in the CFA model at 4 and 8 days after treatment. Notably, ML3403 and CBS-3595 did not show marked signs of hepatoxicity, as supported by unaltered histological observations in the liver sections. Finally, both compounds were safe in the gastrointestinal tract, according to evaluation of intestinal biopsies. CBS-3595 displayed a superior profile regarding its anti-inflammatory effects. Thus p38 MAPK/PDE4 blocking might well constitute a relevant strategy for the treatment of RA.

  18. Transition states for the dimerization of 1,3-cyclohexadiene: a DFT, CASPT2, and CBS-QB3 quantum mechanical investigation.

    Science.gov (United States)

    Ess, Daniel H; Hayden, Amy E; Klärner, Frank-Gerrit; Houk, K N

    2008-10-03

    Quantum mechanical calculations using restricted and unrestricted B3LYP density functional theory, CASPT2, and CBS-QB3 methods for the dimerization of 1,3-cyclohexadiene (1) reveal several highly competitive concerted and stepwise reaction pathways leading to [4 + 2] and [2 + 2] cycloadducts, as well as a novel [6 + 4] ene product. The transition state for endo-[4 + 2] cycloaddition (endo-2TS, DeltaH(double dagger)(B3LYP(0K)) = 28.7 kcal/mol and DeltaH(double dagger)(CBS-QB3(0K)) = 19.0 kcal/mol) is not bis-pericyclic, leading to nondegenerate primary and secondary orbital interactions. However, the C(s) symmetric second-order saddle point on the B3LYP energy surface is only 0.3 kcal/mol above endo-2TS. The activation enthalpy for the concerted exo-[4 + 2] cycloaddition (exo-2TS, DeltaH(double dagger)(B3LYP(0K)) = 30.1 kcal/mol and DeltaH(double dagger)(CBS-QB3(0K)) = 21.1 kcal/mol) is 1.4 kcal/mol higher than that of the endo transition state. Stepwise pathways involving diallyl radicals are formed via two different C-C forming transition states (rac-5TS and meso-5TS) and are predicted to be competitive with the concerted cycloaddition. Transition states were located for cyclization from intermediate rac-5 leading to the endo-[4 + 2] (endo-2) and exo-[2 + 2] (anti-3) cycloadducts. Only the endo-[2 + 2] (syn-3) transition state was located for cyclization of intermediate meso-5. The novel [6 + 4] "concerted" ene transition state (threo-4TS, DeltaH(double dagger)(UB3LYP(0K)) = 28.3 kcal/mol) is found to be unstable with respect to an unrestricted calculation. This diradicaloid transition state closely resembles the cyclohexadiallyl radical rather than the linked cyclohexadienyl radical. Several [3,3] sigmatropic rearrangement transition states were also located and have activation enthalpies between 27 and 31 kcal/mol.

  19. Dissociation of protonated oxalic acid [HOOC-C(OH)2]+ into H3O+ + CO + CO2: An experimental and CBS-QB3 computational study

    Science.gov (United States)

    Ervasti, Henri K.; Lee, Richard; Burgers, Peter C.; Ruttink, Paul J. A.; Terlouw, Johan K.

    2006-03-01

    The predominant dissociation process observed for metastable protonated oxalic acid ions HOOC-C(OH)2+ (generated by self-protonation) leads to H3O++ CO + CO2. We have traced the mechanism of this intriguing reaction using the CBS-QB3 model chemistry. Our calculations show that a unique ter-body complex, OCO...H3O+...CO, plays a key role in the rearrangement process. This complex can also dissociate to the proton bound dimers [H2O...H...OCO]+ and [H2O...H...CO]+ which are minor processes observed in the metastable ion mass spectrum. A further minor process leads to the proton bound dimer OCO...H+...CO which is formed by water extrusion from the ter-body complex. Arguments are provided that the ter-body complex is also generated in the ion source by the collision encounter between neutral and ionized oxalic acid.

  20. G4MP2, DFT and CBS-Q calculation of proton and electron affinities, gas phase basicities and ionization energies of hydroxylamines and alkanolamines

    Indian Academy of Sciences (India)

    Younes Valadbeigi; Hossein Farrokhpour; Mahmoud Tabrizchi

    2014-07-01

    The proton affinities, gas phase basicities and adiabatic ionization energies and electron affinities of some important hydroxylamines and alkanolamines were calculated using B3LYP, CBS-Q and G4MP2 methods. Also, the B3LYP method was used to calculate vertical ionization energies and electron affinities of the molecules. The calculated ionization energies are in the range of 8-10.5 eV and they decrease as the number of carbon atoms increases. Computational results and ion mobility spectrometry study confirm that some alkanolamines lose a water molecule due to protonation at oxygen site and form cationic cyclic compounds. Effect of different substitutions on the cyclization of ethanolamine was studied theoretically.

  1. Cellulase production by Talaromyces emersonii CBS 814. 70 and a mutant UV7 during growth on cellulose, lactose and glucose containing media

    Energy Technology Data Exchange (ETDEWEB)

    Morrison, J.; McCarthy, U.; McHale, A.P.

    1987-07-01

    Recently it has been reported that Talaromyces emersonii CBS 814.70 is capable of growth on lactose containing media. During growth on such media large amounts of beta-glucosidase are secreted into the medium. In addition, low levels of endoglucanase activity have been detected. In order to enhance endoglucanase production, u.v. irradiation and a modified selection procedure yielded a number of mutants. One of these, UV7, was capable of increased cellulase production during growth on cellulose, lactose and glucose containing media. Comparative studies between the wild-type organism and the mutant have shown that the former apparently produces constitutive levels of both endoclucanase and beta-glucosidase. The form of beta-glucosidase that appears to be constitutive is that form previously named BG-1. (Refs. 12).

  2. Het benaderen van de onbetrouwbaarheidsmarges van Onderzoek VerplaatsingsGedrag OVG-cijfers : toepassing van de approximatie-methode van het Centraal Bureau voor de Statistiek CBS. In opdracht van het Directoraat-Generaal Rijkswaterstaat, Adviesdienst Verkeer en Vervoer AVV.

    NARCIS (Netherlands)

    Bijleveld, F.D.

    1999-01-01

    The National Travel Survey (NTS), which is continuous, is carried out by Statistics Netherlands, the Dutch Central Bureau of Statistics (CBS). A sample of the population of the Netherlands is surveyed about their travel behaviour: how many kilometres, which modes of transport, during which period. T

  3. Interactions among methylenetetrahydrofolate reductase (MTHFR) and cystathionine β-synthase (CBS) polymorphisms - a cross-sectional study: multiple heterozygosis as a risk factor for higher homocysteine levels and vaso-occlusive episodes.

    Science.gov (United States)

    Amaral, F M; Miranda-Vilela, A L; Lordelo, G S; Ribeiro, I F; Daldegan, M B; Grisolia, C K

    2017-02-23

    High plasma homocysteine (Hcy) ​​levels may be responsible for vaso-occlusive episodes and may have acquired and/or genetic causes. This cross-sectional study aimed to investigate the role of methylenetetrahydrofolate reductase (MTHFR; C677T; A1298C) and cystathionine-β-synthase (CBS; T833C/844ins68, G919A) polymorphisms in serum levels of folic acid, vitamin B12 and Hcy, and to verify a possible association between these polymorphisms and the clinical variability. Blood samples of Brazilian patients with a diagnosis of thrombosis were submitted to genotyping by PCR-based methods and serum dosages of folic acid, vitamin B12 and Hcy. Except for the CBS G919A polymorphism, all other genetic markers were in Hardy-Weinberg equilibrium. An increased risk for venous thrombosis was found for the MTHFR 1298CC carriers (OR = 1.688; 95%CI = 0.839-3.398, P = 0.018) and those homozygously mutant for the CBS haplotype 844ins68/T833C (OR = 2.488; 95%CI = 0.501-12.363, P = 0.031), while heterozygous for this CBS haplotype showed an increased risk for higher Hcy levels (OR = 5.900; 95%CI = 1.003-34.691, P = 0.030). Significant interactions were observed among the MTHFR C677T, MTHFR A1298C and CBS haplotype 844ins68/T833C polymorphisms in the results for Hcy levels (P = 0.000), where heterozygous had higher values. Interactions among these polymorphisms can affect serum Hcy levels, where multiple heterozygosis could be a risk factor for vaso-occlusive episodes.

  4. Lipid, Oxidative and Inflammatory Profile and Alterations in the Enzymes Paraoxonase and Butyrylcholinesterase in Plasma of Patients with Homocystinuria Due CBS Deficiency: The Vitamin B12 and Folic Acid Importance.

    Science.gov (United States)

    Vanzin, Camila Simioni; Mescka, Caroline Paula; Donida, Bruna; Hammerschimidt, Tatiane Grazieli; Ribas, Graziela S; Kolling, Janaína; Scherer, Emilene B; Vilarinho, Laura; Nogueira, Célia; Coitinho, Adriana Simon; Wajner, Moacir; Wyse, Angela T S; Vargas, Carmen Regla

    2015-08-01

    Cystathionine-β-synthase (CBS) deficiency is the main cause of homocystinuria. Homocysteine (Hcy), methionine, and other metabolites of Hcy accumulate in the body of affected patients. Despite the fact that thromboembolism represents the major cause of morbidity in CBS-deficient patients, the mechanisms of cardiovascular alterations found in homocystinuria remain unclear. In this work, we evaluated the lipid and inflammatory profile, oxidative protein damage, and the activities of the enzymes paraoxonase (PON1) and butyrylcholinesterase (BuChE) in plasma of CBS-deficient patients at diagnosis and during the treatment (protein-restricted diet supplemented with pyridoxine, folic acid, betaine, and vitamin B12). We also investigated the effect of folic acid and vitamin B12 on these parameters. We found a significant decrease in HDL cholesterol and apolipoprotein A1 (ApoA-1) levels, as well as in PON1 activity in both untreated and treated CBS-deficient patients when compared to controls. BuChE activity and IL-6 levels were significantly increased in not treated patients. Furthermore, significant positive correlations between PON1 activity and sulphydryl groups and between IL-6 levels and carbonyl content were verified. Moreover, vitamin B12 was positively correlated with PON1 and ApoA-1 levels, while folic acid was inversely correlated with total Hcy concentration, demonstrating the importance of this treatment. Our results also demonstrated that CBS-deficient patients presented important alterations in biochemical parameters, possibly caused by the metabolites of Hcy, as well as by oxidative stress, and that the adequate adherence to the treatment is essential to revert or prevent these alterations.

  5. Energies and 2'-Hydroxyl Group Orientations of RNA Backbone Conformations. Benchmark CCSD(T)/CBS Database, Electronic Analysis, and Assessment of DFT Methods and MD Simulations.

    Science.gov (United States)

    Mládek, Arnošt; Banáš, Pavel; Jurečka, Petr; Otyepka, Michal; Zgarbová, Marie; Šponer, Jiří

    2014-01-14

    Sugar-phosphate backbone is an electronically complex molecular segment imparting RNA molecules high flexibility and architectonic heterogeneity necessary for their biological functions. The structural variability of RNA molecules is amplified by the presence of the 2'-hydroxyl group, capable of forming multitude of intra- and intermolecular interactions. Bioinformatics studies based on X-ray structure database revealed that RNA backbone samples at least 46 substates known as rotameric families. The present study provides a comprehensive analysis of RNA backbone conformational preferences and 2'-hydroxyl group orientations. First, we create a benchmark database of estimated CCSD(T)/CBS relative energies of all rotameric families and test performance of dispersion-corrected DFT-D3 methods and molecular mechanics in vacuum and in continuum solvent. The performance of the DFT-D3 methods is in general quite satisfactory. The B-LYP-D3 method provides the best trade-off between accuracy and computational demands. B3-LYP-D3 slightly outperforms the new PW6B95-D3 and MPW1B95-D3 and is the second most accurate density functional of the study. The best agreement with CCSD(T)/CBS is provided by DSD-B-LYP-D3 double-hybrid functional, although its large-scale applications may be limited by high computational costs. Molecular mechanics does not reproduce the fine energy differences between the RNA backbone substates. We also demonstrate that the differences in the magnitude of the hyperconjugation effect do not correlate with the energy ranking of the backbone conformations. Further, we investigated the 2'-hydroxyl group orientation preferences. For all families, we conducted a QM and MM hydroxyl group rigid scan in gas phase and solvent. We then carried out set of explicit solvent MD simulations of folded RNAs and analyze 2'-hydroxyl group orientations of different backbone families in MD. The solvent energy profiles determined primarily by the sugar pucker match well with the

  6. The high-capacity specific fructose facilitator ZrFfz1 is essential for the fructophilic behavior of Zygosaccharomyces rouxii CBS 732T.

    Science.gov (United States)

    Leandro, Maria José; Cabral, Sara; Prista, Catarina; Loureiro-Dias, Maria C; Sychrová, Hana

    2014-11-01

    Zygosaccharomyces rouxii is a fructophilic yeast that consumes fructose preferably to glucose. This behavior seems to be related to sugar uptake. In this study, we constructed Z. rouxii single-, double-, and triple-deletion mutants in the UL4 strain background (a ura3 strain derived from CBS 732(T)) by deleting the genes encoding the specific fructose facilitator Z. rouxii Ffz1 (ZrFfz1), the fructose/glucose facilitator ZrFfz2, and/or the fructose symporter ZrFsy1. We analyzed the effects on the growth phenotype, on kinetic parameters of fructose and glucose uptake, and on sugar consumption profiles. No growth phenotype was observed on fructose or glucose upon deletion of FFZ genes. Deletion of ZrFFZ1 drastically reduced fructose transport capacity, increased glucose transport capacity, and eliminated the fructophilic character, while deletion of ZrFFZ2 had almost no effect. The strain in which both FFZ genes were deleted presented even higher consumption of glucose than strain Zrffz1Δ, probably due to a reduced repressing effect of fructose. This study confirms the molecular basis of the Z. rouxii fructophilic character, demonstrating that ZrFfz1 is essential for Z. rouxii fructophilic behavior. The gene is a good candidate to improve the fructose fermentation performance of industrial Saccharomyces cerevisiae strains. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  7. Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit.

    Science.gov (United States)

    Nakatsu, Yusuke; Iwashita, Misaki; Sakoda, Hideyuki; Ono, Hiraku; Nagata, Kengo; Matsunaga, Yasuka; Fukushima, Toshiaki; Fujishiro, Midori; Kushiyama, Akifumi; Kamata, Hideaki; Takahashi, Shin-Ichiro; Katagiri, Hideki; Honda, Hiroaki; Kiyonari, Hiroshi; Uchida, Takafumi; Asano, Tomoichiro

    2015-10-02

    AMP-activated protein kinase (AMPK) plays a critical role in metabolic regulation. In this study, first, it was revealed that Pin1 associates with any isoform of γ, but not with either the α or the β subunit, of AMPK. The association between Pin1 and the AMPK γ1 subunit is mediated by the WW domain of Pin1 and the Thr(211)-Pro-containing motif located in the CBS domain of the γ1 subunit. Importantly, overexpression of Pin1 suppressed AMPK phosphorylation in response to either 2-deoxyglucose or biguanide stimulation, whereas Pin1 knockdown by siRNAs or treatment with Pin1 inhibitors enhanced it. The experiments using recombinant Pin1, AMPK, LKB1, and PP2C proteins revealed that the protective effect of AMP against PP2C-induced AMPKα subunit dephosphorylation was markedly suppressed by the addition of Pin1. In good agreement with the in vitro data, the level of AMPK phosphorylation as well as the expressions of mitochondria-related genes, such as PGC-1α, which are known to be positively regulated by AMPK, were markedly higher with reduced triglyceride accumulation in the muscles of Pin1 KO mice as compared with controls. These findings suggest that Pin1 plays an important role in the pathogenic mechanisms underlying impaired glucose and lipid metabolism, functioning as a negative regulator of AMPK. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Investigation of the effect of glucose syrup and brown sugar as low-cost substrate for lipid production by Mortierella alpine CBS 754.68

    Directory of Open Access Journals (Sweden)

    saed Montazeri

    2016-06-01

    Full Text Available Introduction: Arachidonic acid is an important essential fatty acid in human nutrition. The filamentous fungus Mortierella alpina has been identified as a promising producer of arachidonic acid. Mortierella alpine can accumulate up to 40% (w/w lipid, of which up to 40% can be arachidonic acid. Materials and methods: Mortierella alpina CBS 754.68 was cultivated in low cost substrate such as glucose syrup, brown sugar and starch for lipid and arachidonic acid production. The reduced sugar, total lipids and content of ARA were determined by dinitrosalicylic acid method, soxhlet and Gas chromatography–mass spectrometry (GC-MS respectively. Results: The carbon sources were applied at 70 g/l and nitrogen source (soybean powder at 10 g/lit. The results showed that lipid in dry biomass in glucose syrup, starch and brown sugar media were obtained 32, 25 and 13 % w/w respectively. The arachidonic acid contents of lipid in the glucose syrup, starch and brown sugar media were 41, 33 and 31 % w/w respectively. Discussion and conclusion: Lipid fatty acid compositions are affected by the growth of microorganism. Cell membrane fatty acids such as stearic acid and oleic acid increased substantially concomitant with increases in the amount of biomass. Biomass and oil production efficiency fell due to inappropriate brown sugar medium.

  9. Gibbs energy of activation for thermal isomerization of (1Z)-acetaldehyde hydrazone and (1Z)-acetaldehyde N,N-dimethylhydrazone by Gaussian-4 theory and CCSD(T)/CBS computations.

    Science.gov (United States)

    Lu, Shih-I

    2009-11-15

    In this article, we examined the Gibbs energy of activation for the Z/E thermal isomerization reaction of (1Z)-acetaldehyde hydrazone and (1Z)-acetaldehyde N,N-dimethylhydrazone, at 298.15 K in the solvent of cyclohexane. We carried out computations employing both the Gaussian-4 (G4) theory and the coupled cluster method using both single and double substitutions and triple excitations noniteratively, CCSD(T). The CCSD(T) energy is extrapolated to the complete basis set (CBS). We compared the calculated results to the available experimental observation. It appeared that both G4 and CCSD(T)/CBS computations overestimated the experimental value by as much as about 6 and 12 kcal/mol in the present two cases. We discussed possible sources of error and proposed the experimental kinetic data could be questionable.

  10. Madeup and Application of New CBS Package Material for 66kV Directly-Buried HV Cable%66 kV 直埋高压电缆新型 CBS 包裹料配制及应用

    Institute of Scientific and Technical Information of China (English)

    潘纪良; 李炳汉

    2013-01-01

    The new CBS package material is first applied to the 66 kV directly-buried HV cable of CP1 Project, Qatar.The sand-dune sand which is rich locally is utilized as the raw material of the package one.This not only reduces cost but also is convenient for construc-tion and assures the construction quality.In this paper, one new cable package materials satisfying design requirement, namely, CBS ca-ble package material, is described in accordance with the study on the package material for 66 kV directly-buried HV cable.Meanwhile, one package of construction technology and methods which are worth of developing in construction in the gulf region is concluded .It pro-vides potential sequent similar construction with scientific basis.%卡塔尔路赛CP1项目66 kV直埋式高压电缆采用CBS包裹料尚属首次使用,该包裹料选用了当地丰富沙丘沙为原材料,不但节约了施工成本而且易于施工,质量有保证。文章通过对66 kV直埋高压电缆包裹料研究,配制出了一种满足设计要求的新型电缆包裹料,即CBS电缆包裹料,同时根据CBS料的基本特性,总结出一套在中东海湾地区具有推广价值的施工工艺和施工方法,为后续类似工程施工提供科学的依据。

  11. Complete Genome Sequence of a High Lipid-Producing Strain of Mucor circinelloides WJ11 and Comparative Genome Analysis with a Low Lipid-Producing Strain CBS 277.49.

    Science.gov (United States)

    Tang, Xin; Zhao, Lina; Chen, Haiqin; Chen, Yong Q; Chen, Wei; Song, Yuanda; Ratledge, Colin

    2015-01-01

    The genome of a high lipid-producing fungus Mucor circinelloides WJ11 (36% w/w lipid, cell dry weight, CDW) was sequenced and compared with that of the low lipid-producing strain, CBS 277.49 (15% w/w lipid, CDW), which had been sequenced by Joint Genome Institute. The WJ11 genome assembly size was 35.4 Mb with a G+C content of 39.7%. The general features of WJ11 and CBS 277.49 indicated that they have close similarity at the level of gene order and gene identity. Whole genome alignments with MAUVE revealed the presence of numerous blocks of homologous regions and MUMmer analysis showed that the genomes of these two strains were mostly co-linear. The central carbon and lipid metabolism pathways of these two strains were reconstructed and the numbers of genes encoding the enzymes related to lipid accumulation were compared. Many unique genes coding for proteins involved in cell growth, carbohydrate metabolism and lipid metabolism were identified for each strain. In conclusion, our study on the genome sequence of WJ11 and the comparative genomic analysis between WJ11 and CBS 277.49 elucidated the general features of the genome and the potential mechanism of high lipid accumulation in strain WJ11 at the genomic level. The different numbers of genes and unique genes involved in lipid accumulation may play a role in the high oleaginicity of strain WJ11.

  12. Crystal structure of a hypothetical protein, TTHA0829 from Thermus thermophilus HB8, composed of cystathionine-β-synthase (CBS) and aspartate-kinase chorismate-mutase tyrA (ACT) domains.

    Science.gov (United States)

    Nakabayashi, Makoto; Shibata, Naoki; Ishido-Nakai, Emi; Kanagawa, Mayumi; Iio, Yota; Komori, Hirofumi; Ueda, Yasufumi; Nakagawa, Noriko; Kuramitsu, Seiki; Higuchi, Yoshiki

    2016-05-01

    TTHA0829 from Thermus thermophilus HB8 has a molecular mass of 22,754 Da and is composed of 210 amino acid residues. The expression of TTHA0829 is remarkably elevated in the latter half of logarithmic growth phase. TTHA0829 can form either a tetrameric or dimeric structure, and main-chain folding provides an N-terminal cystathionine-β-synthase (CBS) domain and a C-terminal aspartate-kinase chorismate-mutase tyrA (ACT) domain. Both CBS and ACT are regulatory domains to which a small ligand molecule can bind. The CBS domain is found in proteins from organisms belonging to all kingdoms and is observed frequently as two or four tandem copies. This domain is considered as a small intracellular module with a regulatory function and is typically found adjacent to the active (or functional) site of several enzymes and integral membrane proteins. The ACT domain comprises four β-strands and two α-helices in a βαββαβ motif typical of intracellular small molecule binding domains that help control metabolism, solute transport and signal transduction. We discuss the possible role of TTHA0829 based on its structure and expression pattern. The results imply that TTHA0829 acts as a cell-stress sensor or a metabolite acceptor.

  13. Towards high-level theoretical studies of large biodiesel molecules: an ONIOM [QCISD(T)/CBS:DFT] study of hydrogen abstraction reactions of C(n)H(2n+1)COOC(m)H(2m+1) + H.

    Science.gov (United States)

    Zhang, Lidong; Zhang, Peng

    2015-01-07

    Recent interest in biodiesel combustion urges the need for the theoretical chemical kinetics of large alkyl ester molecules. This is, however, computationally challenging for prevalent high-level electronic structure theory based methods. The hydrogen abstraction reactions of alky esters CnH2n+1COOCmH2m+1 (n = 1-5, 9, 15; m = 1, 2) by a hydrogen radical were investigated by a computational technique based on a two-layer ONIOM method, employing a QCISD(T)/CBS method for the high layer and a DFT method for the low layer. The calculated energy barriers and heats of reaction, using the ONIOM method with a minimum of the required chemically active portion, are in very good agreement with those obtained using the widely accepted high-level QCISD(T)/CBS theory because the computational errors were less than 0.1 kcal mol(-1) for all the tested cases. The ONIOM[QCISD(T)/CBS:DFT] method provides a computationally accurate and affordable approach to the high-level theoretical chemical kinetics of large biodiesel molecules.

  14. Complete Genome Sequence of a High Lipid-Producing Strain of Mucor circinelloides WJ11 and Comparative Genome Analysis with a Low Lipid-Producing Strain CBS 277.49.

    Directory of Open Access Journals (Sweden)

    Xin Tang

    Full Text Available The genome of a high lipid-producing fungus Mucor circinelloides WJ11 (36% w/w lipid, cell dry weight, CDW was sequenced and compared with that of the low lipid-producing strain, CBS 277.49 (15% w/w lipid, CDW, which had been sequenced by Joint Genome Institute. The WJ11 genome assembly size was 35.4 Mb with a G+C content of 39.7%. The general features of WJ11 and CBS 277.49 indicated that they have close similarity at the level of gene order and gene identity. Whole genome alignments with MAUVE revealed the presence of numerous blocks of homologous regions and MUMmer analysis showed that the genomes of these two strains were mostly co-linear. The central carbon and lipid metabolism pathways of these two strains were reconstructed and the numbers of genes encoding the enzymes related to lipid accumulation were compared. Many unique genes coding for proteins involved in cell growth, carbohydrate metabolism and lipid metabolism were identified for each strain. In conclusion, our study on the genome sequence of WJ11 and the comparative genomic analysis between WJ11 and CBS 277.49 elucidated the general features of the genome and the potential mechanism of high lipid accumulation in strain WJ11 at the genomic level. The different numbers of genes and unique genes involved in lipid accumulation may play a role in the high oleaginicity of strain WJ11.

  15. Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen‑glucose deprivation‑induced endoplasmic reticulum stress in PC12 cells.

    Science.gov (United States)

    Zhang, Rui; Lin, Yong-Quan; Wang, Wei-Sheng; Wang, Xin-Qiang

    2017-08-01

    Hypoxic‑ischemia stress causes severe brain injury, leading to death and disability worldwide. Although it has been reported that endoplasmic reticulum (ER) stress is an essential step in the progression of hypoxia or ischemia‑induced brain injury, the underlying molecular mechanisms are and have not yet been fully elucidated. Accumulating evidence has indicated that both nitric oxide (NO) and hydrogen sulfide (H2S) play an important role in the development of cerebral ischemic injury. In the present study, we aimed to investigate the effect of the association between NO signaling and the cystathionine β‑synthase (CBS)/H2S system on ER stress in a cell model of cerebral hypoxia‑ischemia injury. We found that oxygen‑glucose deprivation (OGD) markedly increased the NO level and neuronal NO synthase (nNOS) activity. 3‑Bromo‑7‑nitroindazole (3‑Br‑7‑NI), a relatively selective nNOS inhibitor, abolished the OGD‑induced inhibition of cell viability and the increased expression of ER stress‑related proteins, including glucose‑regulated protein 78 (GRP78), C/EBP homologous protein (CHOP) and cleaved caspase‑12 in PC12 cells, indicating the contribution of excessive nNOS/NO signaling to OGD‑induced ER stress. Furthermore, we found that OGD increased the phosphorylated AMP‑activated protein kinase (p‑AMPK)/AMPK ratio, and the AMPK activator, 5‑aminoimidazole‑4‑carboxamide‑1‑β‑D‑ribofuranoside (AICAR), attenuated the effects on OGD‑induced ER stress, suggesting that OGD‑induced NO overproduction results in AMPK activation in PC12 cells. We also found that OGD induced the downregulation of the CBS/H2S system, as indicated by the decreased H2S level in the culture supernatant and CBS activity in PC12 cells. In addition, we found that treatment with NaHS (a H2S donor) or S‑adenosyl‑L‑methionine (SAM, a CBS agonist) mitigated OGD‑induced ER stress, as well as the NO level, nNOS activity and AMPK

  16. Kinetic and redox properties of MnP II, a major manganese peroxidase isoenzyme from Panus tigrinus CBS 577.79.

    Science.gov (United States)

    Petruccioli, Maurizio; Frasconi, Marco; Quaratino, Daniele; Covino, Stefano; Favero, Gabriele; Mazzei, Franco; Federici, Federico; D'Annibale, Alessandro

    2009-11-01

    A manganese peroxidase (MnP) isoenzyme from Panus tigrinus CBS 577.79 was produced in a benchtop stirred-tank reactor and purified to apparent homogeneity. The purification scheme involving ultrafiltration, affinity chromatography on concanavalin-A Sepharose, and gel filtration led to a purified MnP, termed "MnP II," with a specific activity of 288 IU mg(-1) protein and a final yield of 22%. The enzyme turned out to be a monomeric protein with molecular mass of 50.5 kDa, pI of 4.07, and an extent of N-glycosylation of about 5.3% of the high-mannose type. The temperature and pH optima for the formation of malonate manganic chelates were 45 degrees C and 5.5, respectively. MnP II proved to be poorly thermostable at 50 and 60 degrees C, with half-lives of 11 min and 105 s, respectively. K (m) values for H(2)O(2) and Mn(2+) were 16 and 124 microM, respectively. Although MnP II was able to oxidize veratryl alcohol and to catalyze the Mn(2+)-independent oxidation of several phenols, it cannot be assigned to the versatile peroxidase family. As opposed to versatile peroxidase oxidation, veratryl alcohol oxidation required the simultaneous presence of H(2)O(2) and Mn(2+); in addition, low turnover numbers and K (m) values higher than 300 microM characterized the Mn(2+)-independent oxidation of substituted phenols. Kinetic properties and the substrate specificity of the enzyme markedly differed from those reported for MnP isoenzymes produced by the reference strain P. tigrinus 8/18. To our knowledge, this study reports for the first time a thorough electrochemical characterization of a MnP from this fungus.

  17. Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products.

    Science.gov (United States)

    Ortiz, Gastón E; Guitart, María E; Cavalitto, Sebastián F; Albertó, Edgardo O; Fernández-Lahore, Marcelo; Blasco, Martín

    2015-11-01

    The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94% were optimal for cellulases production. Under these conditions, 8-10 IU gds(-1) of FPA and 15.6-17.8 IU gds(-1) of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds(-1) FPA and 13.5 IU gds(-1) EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe(+2) and a slightly greater tolerance to alkaline pH in comparison to Celluclast(®). These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.

  18. Purification and some properties of component B of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS 3.

    Science.gov (United States)

    Schweizer, D; Markus, A; Seez, M; Ruf, H H; Lingens, F

    1987-07-05

    4-Chlorophenylacetate 3,4-dioxygenase system from Pseudomonas sp. CBS 3 consists of two protein components, a red-brown iron-sulfur protein (component A) which functions as dioxygenase and an orange-colored reductase (component B). Component B was purified by a five-step procedure. Criterion of purity was sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which also showed that the protein consists of one polypeptide species with a molecular weight of 35,000. With gel chromatography on Sephadex G-100 also, a molecular weight of 35,000 was found for the native enzyme, suggesting a monomeric structure for the reductase enzyme. The isoelectric point was determined as pH 4.8. The visible absorption spectrum of the homogeneous protein exhibits maxima at 336, 394, and 458 nm. One mol of FMN, 2.1 mol of iron, and 1.7 mol of acid-labile sulfide were found in one mol of component B. The EPR-spectrum of the reduced form of the enzyme (with NADH) showed two types of signals. The signal at g values of 2.03, 1.94, and 1.90 was assigned to an iron-sulfur cluster of the [2Fe-2S]-type. The properties of the other signal type at g = 2.004 are characteristic of flavosemiquinone radical which does not show coupling to an other paramagnetic center. The apparent Km values for 2,6-dichlorophenolindophenol, cytochrome c, and NADH were calculated from Lineweaver-Burk plots as 6.3, 2.3, and 32 microM, respectively. Inhibitors of the reductase are metal-chelating reagents and sulfhydryl-inhibiting compounds. Component B reduces several redox compounds: 2,6-dichlorophenolindophenol, potassium hexacyanoferrat III, cytochrome c, methylene blue, and nitro blue tetrazolium.

  19. Measuring $\

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, Jessica Sarah [Univ. of Cambridge (United Kingdom)

    2011-01-01

    The MINOS Experiment consists of two steel-scintillator calorimeters, sampling the long baseline NuMI muon neutrino beam. It was designed to make a precise measurement of the ‘atmospheric’ neutrino mixing parameters, Δm2 atm. and sin2 (2 atm.). The Near Detector measures the initial spectrum of the neutrino beam 1km from the production target, and the Far Detector, at a distance of 735 km, measures the impact of oscillations in the neutrino energy spectrum. Work performed to validate the quality of the data collected by the Near Detector is presented as part of this thesis. This thesis primarily details the results of a vμ disappearance analysis, and presents a new sophisticated fitting software framework, which employs a maximum likelihood method to extract the best fit oscillation parameters. The software is entirely decoupled from the extrapolation procedure between the detectors, and is capable of fitting multiple event samples (defined by the selections applied) in parallel, and any combination of energy dependent and independent sources of systematic error. Two techniques to improve the sensitivity of the oscillation measurement were also developed. The inclusion of information on the energy resolution of the neutrino events results in a significant improvement in the allowed region for the oscillation parameters. The degree to which sin2 (2θ )= 1.0 could be disfavoured with the exposure of the current dataset if the true mixing angle was non-maximal, was also investigated, with an improved neutrino energy reconstruction for very low energy events. The best fit oscillation parameters, obtained by the fitting software and incorporating resolution information were: | Δm2| = 2.32+0.12 -0.08×10-3 eV2 and sin2 (2θ ) > 0.90(90% C.L.). The analysis provides the current world best measurement of the atmospheric neutrino mass

  20. Probing the crucial role of Leu31 and Thr33 of the Bacillus pumilus CBS alkaline protease in substrate recognition and enzymatic depilation of animal hide.

    Directory of Open Access Journals (Sweden)

    Nadia Zaraî Jaouadi

    Full Text Available The sapB gene, encoding Bacillus pumilus CBS protease, and seven mutated genes (sapB-L31I, sapB-T33S, sapB-N99Y, sapB-L31I/T33S, sapB-L31I/N99Y, sapB-T33S/N99Y, and sapB-L31I/T33S/N99Y were overexpressed in protease-deficient Bacillus subtilis DB430 and purified to homogeneity. SAPB-N99Y and rSAPB displayed the highest levels of keratinolytic activity, hydrolysis efficiency, and enzymatic depilation. Interestingly, and at the semi-industrial scale, rSAPB efficiently removed the hair of goat hides within a short time interval of 8 h, thus offering a promising opportunity for the attainment of a lime and sulphide-free depilation process. The efficacy of the process was supported by submitting depilated pelts and dyed crusts to scanning electron microscopic analysis, and the results showed well opened fibre bundles and no apparent damage to the collagen layer. The findings also revealed better physico-chemical properties and less effluent loads, which further confirmed the potential candidacy of the rSAPB enzyme for application in the leather industry to attain an ecofriendly process of animal hide depilation. More interestingly, the findings on the substrate specificity and kinetic properties of the enzyme using the synthetic peptide para-nitroanilide revealed strong preferences for an aliphatic amino-acid (valine at position P1 for keratinases and an aromatic amino-acid (phenylalanine at positions P1/P4 for subtilisins. Molecular modeling suggested the potential involvement of a Leu31 residue in a network of hydrophobic interactions, which could have shaped the S4 substrate binding site. The latter could be enlarged by mutating L31I, fitting more easily in position P4 than a phenylalanine residue. The molecular modeling of SAPB-T33S showed a potential S2 subside widening by a T33S mutation, thus suggesting its importance in substrate specificity.

  1. Yerleşime uygunluğun Coğrafi Bilgi Sistemleri (CBS ile sorgulanması, Esenler (İstanbul örneği

    Directory of Open Access Journals (Sweden)

    T. Fikret Kurnaz

    2014-12-01

    Full Text Available Yerleşim alanlarının planlamalarında, zemin özellikleri açısından güvenli ve riskli olan bölgelerin tespit edilmesinin gerekliliği, özellikle deprem, heyelan vb. doğal afetler göz önüne alındığında oldukça önem arz etmektedir. Özellikle 1999 Marmara depreminde yaşanan kayıpların ardından, ülkemizde yeni yerleşim alanlarının seçiminde ve mevcut yerleşim alanlarının kullanım açısından planlanmasında, Coğrafi Bilgi Sistemleri’nden (CBS yararlanılarak hazırlanan çalışmalar etkin bir rol almaktadır. CBS’nin sağladığı en önemli avantajlarından birisi, oluşturulan veri tabanları içinde farklı türde sorgulamalar yapılabilmesidir. Bu çalışmada, İstanbul’un Avrupa yakasında yer alan Esenler ilçesinin zemin özellikleri jeolojik, jeofizik ve jeoteknik verilerin bir arada kullanımı ile araştırılmış ve yerleşime uygunluk durumu CBS’nin sorgulama özelliği ile değerlendirilmiştir. Bu değerlendirmede, ilçe zeminlerine ait SPT-N30 darbe sayıları, kayma dalgası hızları (Vs, yerel zemin sınıfları, taşıma gücü (qnet, zemin büyütme ve yamaç stabilite durumları kullanılmıştır. Araştırma sonuçlarına göre, ilçenin kuzey kesimleri, yerleşime uygunluk açısından güney kesimlere oranla çok daha güvenilir jeoteknik koşullara sahiptir.

  2. Probing the crucial role of Leu31 and Thr33 of the Bacillus pumilus CBS alkaline protease in substrate recognition and enzymatic depilation of animal hide.

    Science.gov (United States)

    Zaraî Jaouadi, Nadia; Jaouadi, Bassem; Ben Hlima, Hajer; Rekik, Hatem; Belhoul, Mouna; Hmidi, Maher; Ben Aicha, Houda Slimene; Hila, Chiraz Gorgi; Toumi, Abdessatar; Aghajari, Nushin; Bejar, Samir

    2014-01-01

    The sapB gene, encoding Bacillus pumilus CBS protease, and seven mutated genes (sapB-L31I, sapB-T33S, sapB-N99Y, sapB-L31I/T33S, sapB-L31I/N99Y, sapB-T33S/N99Y, and sapB-L31I/T33S/N99Y) were overexpressed in protease-deficient Bacillus subtilis DB430 and purified to homogeneity. SAPB-N99Y and rSAPB displayed the highest levels of keratinolytic activity, hydrolysis efficiency, and enzymatic depilation. Interestingly, and at the semi-industrial scale, rSAPB efficiently removed the hair of goat hides within a short time interval of 8 h, thus offering a promising opportunity for the attainment of a lime and sulphide-free depilation process. The efficacy of the process was supported by submitting depilated pelts and dyed crusts to scanning electron microscopic analysis, and the results showed well opened fibre bundles and no apparent damage to the collagen layer. The findings also revealed better physico-chemical properties and less effluent loads, which further confirmed the potential candidacy of the rSAPB enzyme for application in the leather industry to attain an ecofriendly process of animal hide depilation. More interestingly, the findings on the substrate specificity and kinetic properties of the enzyme using the synthetic peptide para-nitroanilide revealed strong preferences for an aliphatic amino-acid (valine) at position P1 for keratinases and an aromatic amino-acid (phenylalanine) at positions P1/P4 for subtilisins. Molecular modeling suggested the potential involvement of a Leu31 residue in a network of hydrophobic interactions, which could have shaped the S4 substrate binding site. The latter could be enlarged by mutating L31I, fitting more easily in position P4 than a phenylalanine residue. The molecular modeling of SAPB-T33S showed a potential S2 subside widening by a T33S mutation, thus suggesting its importance in substrate specificity.

  3. Chemical Composition and Medicinal Value of Fruiting Bodies and Submerged Cultured Mycelia of Caterpillar Medicinal Fungus Cordyceps militaris CBS-132098 (Ascomycetes).

    Science.gov (United States)

    Chan, Jannie Siew Lee; Barseghyan, Gayane S; Asatiani, Mikheil D; Wasser, Solomon P

    2015-01-01

    In this paper, we report the results of a proximate analysis (i.e., moisture, ash, protein, fat, carbohydrates, and energy); a bioactive compounds analysis (i.e., cordycepin and ergothioneine); fatty and amino acid analysis; and analyses of vitamin content, macro- and microelement composition of fruiting body (FB), and mycelial biomass (MB) of medicinal caterpillar fungus Cordyceps militaris strain CBS-132098. These results demonstrate that the FB and MB of C. militaris are good sources of proteins: 59.8% protein content in the FB and 39.5% in the MB. The MB was distinguished by its carbohydrate content (39.6%), which was higher than that of the FB (29.1% carbohydrate). In the FB of C. militaris, the total amino acid content was 57.39 mg/g and in the MB it was 24.98 mg/g. The quantification of the identified fatty acids indicated that palmitic acid, oleic acid, linoleic acid, and linolenic acid were the major fatty acids. The micro- and macroelement compositions were studied. The highest results were calcium (797 mg/kg FB; 11 mg/kg MB); potassium (15,938 mg/kg FB 12,183 mg/kg MB); magnesium (4,227 mg/kg FB; 3,414 mg/kg MB); sodium (171 mg/kg FB; 1,567 mg/kg MB); phosphorus (7,196 mg/kg FB; 14,293 mg/kg MB); and sulfur (5,088 mg/kg FB; 2,558 mg/kg MB). The vitamin composition was studied, and the most abundant vitamins were vitamin A, vitamin B3, and vitamin E. The bioactive components were cordycepin, cordycepic acid (D-mannitol), and ergothioneine. There were differences in cordycepin and ergothioneine contents between the FB and the MB. The cordycepin concentration was 0.11% in the FB and 0.182% in the MB, the cordycepic acid was 4.7 mg/100g in the FB and 5.2 mg/100 g in the MB, and the ergothioneine content was 782.37 mg/kg in the FB and 130.65 mg/kg in the MB. The nutritional values of the FB and the MB of C. militaris detected indicate its potential use in well-balanced diets and sources of bioactive compounds.

  4. Therapeutic effects of L-Cysteine in newborn mice subjected to hypoxia-ischemia brain injury via the CBS/H2S system: Role of oxidative stress and endoplasmic reticulum stress.

    Science.gov (United States)

    Liu, Song; Xin, Danqing; Wang, Lingxiao; Zhang, Tiantian; Bai, Xuemei; Li, Tong; Xie, Yunkai; Xue, Hao; Bo, Shishi; Liu, Dexiang; Wang, Zhen

    2017-10-01

    Neonatal hypoxic-ischemic (HI) injury is a major cause of neonatal death and neurological dysfunction. H2S has been shown to protect against hypoxia-induced injury and apoptosis of neurons. L-Cysteine is catalyzed by cystathionine-β-synthase (CBS) in the brain and sequentially produces endogenous H2S. The present study was designed to investigate whether L-Cysteine could attenuate the acute brain injury and improve neurobehavioral outcomes following HI brain injury in neonatal mice by releasing endogenous H2S. L-Cysteine treatment significantly attenuated brain edema and decreased infarct volume and neuronal cell death, as shown by a decrease in the Bax/Bcl-2 ratio, suppression of caspase-3 activation, and reduced phosphorylation of Akt and ERK at 72h after HI. Additionally, L-Cysteine substantially up-regulated NF-E2-related factor 2 and heme oxygenase-1 expression. L-Cysteine also decreased endoplasmic reticulum (ER) stress-associated pro-apoptotic protein expression. Furthermore, L-Cysteine had long-term effects by protecting against the loss of ipsilateral brain tissue and improving neurobehavioral outcomes. Importantly, pre-treatment with a CBS inhibitor significantly attenuated the neuroprotection of L-Cysteine on HI insult. Thus, L-Cysteine exerts neuroprotection against HI-induced injury in neonates via the CBS/H2S pathway, mediated in part by anti-apoptotic effects and reduced oxidative stress and ER stress. Thus, L-Cysteine may be a promising treatment for HI. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  5. Relationship of the MTHFD1 (rs2236225, eNOS (rs1799983, CBS (rs2850144 and ACE (rs4343 gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects

    Directory of Open Access Journals (Sweden)

    Mehri Khatami

    2017-09-01

    Full Text Available Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case–control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively. The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD.

  6. Relationship of the MTHFD1 (rs2236225), eNOS (rs1799983), CBS (rs2850144) and ACE (rs4343) gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects.

    Science.gov (United States)

    Khatami, Mehri; Ratki, Farzaneh Morteza; Tajfar, Saba; Akrami, Fatemeh

    2017-09-01

    Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs) in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case-control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively). The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD. Copyright © 2017. Published by Elsevier Taiwan.

  7. Therapeutic effects of L-Cysteine in newborn mice subjected to hypoxia-ischemia brain injury via the CBS/H2S system: Role of oxidative stress and endoplasmic reticulum stress

    Directory of Open Access Journals (Sweden)

    Song Liu

    2017-10-01

    Full Text Available Neonatal hypoxic-ischemic (HI injury is a major cause of neonatal death and neurological dysfunction. H2S has been shown to protect against hypoxia-induced injury and apoptosis of neurons. L-Cysteine is catalyzed by cystathionine-β-synthase (CBS in the brain and sequentially produces endogenous H2S. The present study was designed to investigate whether L-Cysteine could attenuate the acute brain injury and improve neurobehavioral outcomes following HI brain injury in neonatal mice by releasing endogenous H2S. L-Cysteine treatment significantly attenuated brain edema and decreased infarct volume and neuronal cell death, as shown by a decrease in the Bax/Bcl-2 ratio, suppression of caspase-3 activation, and reduced phosphorylation of Akt and ERK at 72 h after HI. Additionally, L-Cysteine substantially up-regulated NF-E2-related factor 2 and heme oxygenase-1 expression. L-Cysteine also decreased endoplasmic reticulum (ER stress-associated pro-apoptotic protein expression. Furthermore, L-Cysteine had long-term effects by protecting against the loss of ipsilateral brain tissue and improving neurobehavioral outcomes. Importantly, pre-treatment with a CBS inhibitor significantly attenuated the neuroprotection of L-Cysteine on HI insult. Thus, L-Cysteine exerts neuroprotection against HI-induced injury in neonates via the CBS/H2S pathway, mediated in part by anti-apoptotic effects and reduced oxidative stress and ER stress. Thus, L-Cysteine may be a promising treatment for HI.

  8. The 'grey' contributions to StatLine. Synthesis of CBS-data and ECN knowledge with regard to conversion and consumption of energy; De 'grijze' bijdragen aan StatLine. Synthese van CBS-data en ECN-kennis met betrekking tot omzetting en verbruik van energie

    Energy Technology Data Exchange (ETDEWEB)

    Van Arkel, W.G. [ECN Beleidsstudies, Petten (Netherlands)

    2000-07-01

    In the period 1990-1999 the Dutch database NEEDIS (National Energy and Efficiency Data Information System) was set up and maintained. The purpose of NEEDIS was to compile and maintain a consistent framework in the field of energy consumption and energy conservation. The title system is developed and maintained by ECN Policy Studies. In the NEEDIS database a large amount of energy consumption data on different aggregation levels (i.e. type of energy carrier, consuming sector, energy function and type of installation) has been inputted. Based on energy consumption the main groups are determined and described in more detail with regard to the production processes, the size of the companies involved and its economic parameters as value added and turnover. Data are presented on the specific energy consumption for the most important (energy consuming) processes and ways to improve energy conservation outlined. During the project a direct link has been established between the presentation of energy data on the internet-site StatLine of Statistics Netherlands (CBS) and the corresponding, so called 'grey data', from the EDN (Energy Databank Netherlands) internet-site of ECN. These data concern more detailed items on energy use, production, building specifications, etc. In this report an overview is given of the available 'grey data' on the ECN-site. Special attention is paid to the energy use of buildings. 70 refs.

  9. Upregulation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway.

    Science.gov (United States)

    Wang, Xiang-Yu; Yang, Hong-Wei

    2016-08-01

    Glutamate-induced neurotoxicity involves in overproduction of nitric oxide (NO) and oxidative stress. Our previous data demonstrated that asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS) inhibitor, has a protective effect against glutamate-induced neurotoxicity. Hydrogen sulfide (H2S), the third endogenous gaseous mediator, has potential therapeutic value for oxidative stress-induced neural damage. Therefore, we hypothesized that ADMA provides protection against the neurotoxicity of glutamate by regulating endogenous H2S generation. In the present study, we found that ADMA prevented glutamate-triggered decrease in endogenous H2S generation in PC12 cells and reversed glutamate-induced suppression in the expression and activity of cystathionine-β-synthetase (CBS), the predominant enzymatic source of H2S in PC12 cells. Furthermore, AOAA, a potent inhibitor of CBS, significantly abolished the protective action of ADMA against glutamate-induced neurotoxicity to PC12 cells. We also showed that ADMA suppressed glutamate-elicited NOS excessive activation and NO overproduction in PC12 cells. These data indicate that the protection of ADMA against glutamate-induced neurotoxicity is by promoting endogenous H2S generation, resulting from suppression in NOS excessive activation and NO overproduction. These findings provide a novel mechanism underlying the protection of ADMA against glutamate-induced neurotoxicity. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Acidities of arsenic (III) and arsenic (V) thio- and oxyacids in aqueous solution using the CBS-QB3/CPCM method.

    Science.gov (United States)

    Zimmermann, Merle D; Tossell, John A

    2009-04-30

    A number of calculations of acidic As(III) and As(V) species formed with sulfur and oxygen (H(3)AsS(3), H(2)AsS(3)(-), HAsS(3)(2-), AsS(3)(3-), H(3)AsS(4), H(2)AsS(4)(-), HAsS(4)(2-), AsS(4)(3-), H(3)AsO(3), H(2)AsO(3)(-), HAsO(3)(2-), AsO(3)(3-), H(3)AsO(4), H(2)AsO(4)(-), HAsO(4)(2-), and AsO(4)(3-)) are presented. pK(a)s for successive deprotonations in both the gas phase and aqueous solution (using both explicit water molecules and a self-consistent reactive field conductive polarizable continuum model (SCRF CPCM) for solvation) are fitted to known experimental values for the H(3)PO(4) and H(3)AsO(4) series' of deprotonations with a linear extrapolation showing r(2) values of 0.97 for the CBS-QB3 method with a single explicit water molecule in the CPCM. Though the unfitted pK(a)s of H(3)AsO(4) (pK(a1) = 4.6, pK(a2) = 17.7, and pK(a3) = 28.6) compare unfavorably with experimental values of pK(a1) = 2.3, pK(a2) = 7, pK(a3) = 13, by the linear fit: 0.42907 x pK(a) - 0.23, the predicted pK(a)s become pK(a1) = 1.7, pK(a2) = 7.3, and pK(a3) = 12.1, which correspond well. The H(3)PO(4) calculation shows a similar trend; pK(a1) = 4.9, pK(a2) = 19.9, and pK(a3) = 29.8 becomes pK(a1) = 1.9, pK(a2) = 8.3, pK(a3) = 12.6. Experimentally, H(3)PO(4) has pK(a1) = 2.2, pK(a2) = 7.1, and pK(a3) = 12.3. With the same extrapolation, we predict H(2)SO(3) to have pK(a1) = 1.1 and pK(a2) = 8.1, which compare favorably with the experimental values of pK(a1) = 1.9 and pK(a2) = 7.2.

  11. Comparison of flux measurement by open-path and close-path eddy covariance systems

    Institute of Scientific and Technical Information of China (English)

    SONG; Xia; YU; Guirui; LIU; Yunfen; SUN; Xiaomin; REN; Chua

    2005-01-01

    For flux measurement, the eddy covariance technique supplies a possibility to directly measure the exchange between vegetation and atmosphere; and there are two kinds of eddy covariance systems, open-path and close-path systems. For the system error, it may result in difference of flux measurements by two systems. Therefore, it is necessary to compare the measured results from them. ChinaFLUX covers of eight sites applied the micrometeorological method, in which Changbai Mountains (CBS) and Qianyanzhou (QYZ) carried out open-path eddy covariance (OPEC) and close-path eddy covariance (CPEC) measurements synchronously.In this paper the data sets of CBS and QYZ were employed. The delay time of close-path analyzer to the open-path analyzer was calculated; the spectra and cospectra of time-series data of OPEC and CPEC were analyzed; the open-path flux measurement was used as a standard comparison, the close-path flux measurement results were evaluated. The results show that, at two sites the delay time of CO2 density for close-path analyzer was about 7.0-8.0 s, H2O density about 8.0-9.0 s; the spectrum from the open-path, close-path and 3D sonic anemometer was consistent with the expected -2/3 slope (log-log plot), and the cospectra showed the expected slope of -4/3 in the internal subrange; the CO2 flux measured by the close-path sensor was about 84% of that of open-path measurement at QYZ, about 80% at CBS, and the latent heat flux was balanced for two systems at QYZ, 86% at CBS. From the flux difference between open-path and close-path analyzers, it could be inferred that the attenuation of turbulent fluctuations in flow through tube of CPEC affected H2O flux more significantly than CO2 flux. The gap between two systems was bigger at CBS than at QYZ; the diurnal variation in CO2 flux of two measurement systems was very consistent.

  12. Mechanism of pH-dependent decomposition of monoalkylamine diazeniumdiolates to form HNO and NO, deduced from the model compound methylamine diazeniumdiolate, density functional theory, and CBS-QB3 calculations.

    Science.gov (United States)

    Dutton, Andrew S; Suhrada, Christopher P; Miranda, Katrina M; Wink, David A; Fukuto, Jon M; Houk, K N

    2006-03-20

    Isopropylamine diazeniumdiolate, IPA/NO, the product of the reaction of isopropylamine and nitric oxide, NO, decomposes in a pH-dependent manner to afford nitroxyl, HNO, in the pH range of 13 to above 5, and NO below pH 7. Theoretical studies using B3LYP/6-311+G(d) density functional theory, the polarizable continuum and conductor-like polarizable continuum solvation models, and the high-accuracy CBS-QB3 method on the simplified model compound methylamine diazeniumdiolate predict a mechanism involving HNO production via decomposition of the unstable tautomer MeNN+(O-)NHO-. The production of NO at lower pH is predicted to result from fragmentation of the amide/NO adduct upon protonation of the amine nitrogen.

  13. Mechanism of pH Dependent Decomposition of Monoalkylamine Diazeniumdiolates to Form HNO and NO, Deduced from the Model Compound Methylamine Diazeniumdiolate, Density Functional Theory and CBS-QB3 Calculations

    Science.gov (United States)

    Dutton, Andrew S.; Suhrada, Christopher P.; Miranda, Katrina M.; Wink, David A.; Fukuto, Jon M.; Houk, K. N.

    2011-01-01

    Isopropylamine diazeniumdiolate, IPA/NO, the product of the reaction of isopropylamine and nitric oxide, NO, decomposes in a pH dependent manner to afford nitroxyl, HNO, in the pH range of 13 to above 5 and NO below pH 7. Theoretical studies using B3LYP/6-311+G(d) density functional theory, the PCM and CPCM solvation models and the high accuracy CBS-QB3 method on the simplified model compound, methylamine diazeniumdiolate, predict a mechanism involving HNO production via decomposition of the unstable tautomer MeNN+(O−)NHO−. The production of NO at lower pH is predicted to result from fragmentation of the amide/NO adduct upon protonation of the amine nitrogen. PMID:16529464

  14. Effect of combination of CBS and PBL in teaching rounds led by nursing students%CBS联合PBL用于实习护生主查房教学的研究

    Institute of Scientific and Technical Information of China (English)

    张雅丽; 汪小冬; 章丽丽

    2011-01-01

    目的 探讨以病例为引导(CBS)联合以问题为基础的学习(PBL)模式指导下的实习护生主查房在临床带教中的应用效果.方法 将轮转实习的护生80名随机分成观察组和对照组各40名.观察组采用以护生为主查人的教学查房,查房中应用CBS联合PBL模式;对照组常规由护生参与科室组织的教学查房.均每周1次.结果 观察组护生出科考试成绩、患者对护生的评价、带教老师对护生的评价和护生实习结束后自我评价显著优于对照组(P<0.05,P<0.01).结论 护生主查房充分调动护生主动思考和学习,将CBS和PBL联合应用有助于临床实践和理论知识相结合,提高护生的学习针对性,提高学习效率,并深化记忆,充分调动护生的学习兴趣,还能培养和提高与患者之间的亲和力,增强护生的自我价值感.%Objective To study the effects of combination of case-based study (CBS) and problem-based learning (PBL) in teaching rounds led by nursing students. Methods A total of 80 nursing interns who did clinical rotation, were randomly divided into an observation group and a control group, with 40 students in each group. The observation group students led the teaching rounds during which CBS and PBL were employed, while their counterparts in the control group participated into the teaching rounds organized by clinical mentors working in the same departments. Teaching rounds were held once a week. Results Compared with the control group, the observation group achieved higher scores in examinations taken before they switched their rotation to another departments, and they received higher appraisal from patients and teachers; they also had higher recognition about themselves (P< 0. 05,P<0. 01). Conclusion Teaching rounds led by nursing students fully exhibit their activeness in thinking and learning. Combination of CBS and PBL enables the integration of theory into clinical practice, which is specifically targeted to

  15. Application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product).

    Science.gov (United States)

    Kammoun, Radhouane; Naili, Belgacem; Bejar, Samir

    2008-09-01

    The production optimization of alpha-amylase (E.C.3.2.1.1) from Aspergillus oryzae CBS 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. The optimisation of temperature, agitation and inoculum size was attempted using a Box-Behnken design under the response surface methodology. The screening of nineteen nutrients for their influence on alpha-amylase production was achieved using a Plackett-Burman design. KH(2)PO(4), urea, glycerol, (NH(4))(2)SO(4), CoCl(2), casein hydrolysate, soybean meal hydrolysate, MgSO(4) were selected based on their positive influence on enzyme formation. The optimized nutrients concentration was obtained using a Taguchi experimental design and the analysis of the data predicts a theoretical increase in the alpha-amylase expression of 73.2% (from 40.1 to 151.1 U/ml). These conditions were validated experimentally and revealed an enhanced alpha-amylase yield of 72.7%.

  16. A Response Surface Methodology Approach to Investigate the Effect of Sulfur Dioxide, pH, and Ethanol on DbCD and DbVPR Gene Expression and on the Volatile Phenol Production in Dekkera/Brettanomyces bruxellensis CBS2499.

    Science.gov (United States)

    Valdetara, Federica; Fracassetti, Daniela; Campanello, Alessia; Costa, Carlo; Foschino, Roberto; Compagno, Concetta; Vigentini, Ileana

    2017-01-01

    Dekkera/Brettanomyces bruxellensis, the main spoilage yeast in barrel-aged wine, metabolize hydroxycinnamic acids into off-flavors, namely ethylphenols. Recently, both the enzymes involved in this transformation, the cinnamate decarboxylase (DbCD) and the vinylphenol reductase (DbVPR), have been identified. To counteract microbial proliferation in wine, sulfur dioxide (SO2) is used commonly to stabilize the final product, but limiting its use is advised to preserve human health and boost sustainability in winemaking. In the present study, the influence of SO2 was investigated in relation with pH and ethanol factors on the expression of DbCD and DbVPR genes and volatile phenol production in D. bruxellensis CBS2499 strain under different model wines throughout a response surface methodology (RSM). In order to ensure an exact quantification of DbCD and DbVPR expression, an appropriate housekeeping gene was sought among DbPDC, DbALD, DbEF, DbACT, and DbTUB genes by GeNorm and Normfinder algorithms. The latter gene showed the highest expression stability and it was chosen as the reference housekeeping gene in qPCR assays. Even though SO2 could not be commented as main factor because of its statistical irrelevance on the response of DbCD gene, linear interactions with pH and ethanol concurred to define a significant effect (p bruxellensis spoilage.

  17. The study on MTHFR and CBS gene polymorphisms,plasma homocysteine concentration and metabolic related factors in Chinese isolated systolic hypertension patients%单纯收缩期高血压患者MTHFR、CBS基因多态性及代谢相关因子研究

    Institute of Scientific and Technical Information of China (English)

    李玉明; 孙晓楠; 郭宏

    2003-01-01

    Objective:To study the relationship between isolated systolic hypertenson and MTHFR,CBS the keys enzyme of homocysteine metabolism,gene polymorphisms,related variation of homocysteine level,their metabolic factors,so as to screen the predisposing gene and intermediate phenotype of ISH.Methods:From Tianjin hypertension prevention and cure area selected by examinations to get 55 examples,matched control 46 example,apply the molecular biology method to detect MTHFR C677,CBS G919,T833,C341 different site gene polymorphisms,and measurese the concentration of Hcy,folic acid,vitamin B12 and B6 at the same time,then proceed the relativity analysis.Results:The genotype frequency of ISH group is different from that of control group(P<0.05).The higher frequency of T allele of MTHFR is 69.1%,observed in ISH group(P<0.01); and the mutation allelt frequency of CBS is 15.2%,different from matched control.The Hcy levels of ISH group(17.7±8.5μmol s/l)are higher than those of matched control(10.65±3.1μmol s/l,P<0.05).But the concentration of folic acid,vitamin B12 and B6 are lower compared to the controlled(P<0.05).There are more hyperhomocysteinemia patients in ISH group(P<0.05). Plasma homocyeteine play apernicious role in ISH pathogeine and folic acid may have negative effect on ISH.Conclusion:Mutation of MTHFR or CBS can both cause the elevation of plasma homocysteine so as to boost the onset of ISH.Then we can draw the conclusion that MTHFR and CBS genes may be the predisposing gene of ISH,Hcy my be the improtant intermiediate phenotype.Reasonable complementation of folic acid,vitamin B6,vitaminB12 can lower the plasma Hcy level,and defer ISH occurrence and progress.

  18. 高分辨率熔解曲线法检测CBS基因SNP的技术优化%The Optimization of High Resolution Melting Analysis for SNP of CBS Gene Detection

    Institute of Scientific and Technical Information of China (English)

    于坤坤; 刘毅; 张坤; 李栋; 马衍辉

    2014-01-01

    Objective To explore the optimal reaction condition of high resolution melting (HRM)for detecting SNPs of Cystathionine Beta Synthetase(CBS),and establish the fast and effective way of genotyping.Methods The annealing temperature range was firstly determined by common polymerase chain reaction,then optimal annealing tem-perature was adjusted by qPCR-HRM.The qPCR-HRM reaction system,including reaction process,DNA template,and MgCl2 concentration,were optimized to obtain the best result.Finally,the genotyping results were varified by Sanger sequencing.In this way,the qPCR-HRM of detecting CBS SNP was established.Results The optimal annealing tem-perature was 62℃;the best reaction system of qPCR-HRM includs primers 0.2μmol/L,Mg2+ 2.5μmol/L,and DNA template 60 ng in 20μl.The results of HRM and direct sequencing were consistent.Conclusion HRM could serve as an accurate,fast,and effective method for detecting SNPs of genes.%目的:探索高分辨率熔解曲线法(high resolution melting ,HRM)检测胱硫醚β-合酶(CBS)基因 SNP位点所需的最佳反应体系和条件,建立快速高效的对CBS基因分型的方法。方法通过普通 PCR初步确立引物的退火温度范围,在实时荧光定量PCR(qPCR)-HRM反应中调整确定最佳退火温度。对影响 qPCR-HRM的因素包括反应程序、模板DNA、MgCl2再分别进行优化,确立最佳反应体系和反应条件,对在此基础上得出的基因分型结果通过测序验证其准确性,从而建立系统的 HRM检测CBS基因SNP的方法。结果 HRM检测CBS基因该片段最佳退火温度为62℃,qPCR-HRM最佳反应体系为20μl,引物浓度为0.2μmol/L,Mg2+为2.5μmol/L,模板DNA为60 ng。在优化的体系条件下筛选出的突变型样本经测序验证与实验结果一致。结论 HRM可以作为检测 SNP准确、经济、高效的方法。

  19. Functional analysis and transcriptional regulation of two orthologs of ARO10, encoding broad-substrate-specificity 2-oxo-acid decarboxylases, in the brewing yeast Saccharomyces pastorianus CBS1483.

    Science.gov (United States)

    Bolat, Irina; Romagnoli, Gabriele; Zhu, Feibai; Pronk, Jack T; Daran, Jean-Marc

    2013-09-01

    The hybrid genomes of Saccharomyces pastorianus consist of subgenomes similar to those of S. cerevisiae and S. eubayanus, and impact of the genome structure on flavour production and its regulation is poorly understood. This study focuses on ARO10, a 2-oxo-acid decarboxylase involved in production of higher alcohols. In S. pastorianus CBS1483, four ARO10 copies were identified, three resembled S. cerevisiae ARO10 and one S. eubayanus ARO10. Substrate specificities of lager strain (Lg)ScAro10 and LgSeubAro10 were compared by individually expressing them in a pdc1Δ-pdc5Δ-pdc6Δ-aro10Δ-thi3Δ S. cerevisiae strain. Both isoenzymes catalysed decarboxylation of the 2-oxo-acids derived from branched-chain, sulphur-containing amino acids and preferably phenylpyruvate. Expression of both alleles was induced by phenylalanine, however in contrast to the S. cerevisiae strain, the two genes were not induced by leucine. Additionally, LgSeubARO10 showed higher basal expression levels during growth with ammonia. ARO80, which encodes ARO10 transcriptional activator, is located on CHRIV and counts three Sc-like and one Seub-like copies. Deletion of LgSeubARO80 did not affect LgSeubARO10 phenylalanine induction, revealing 'trans' regulation across the subgenomes. ARO10 transcript levels showed a poor correlation with decarboxylase activities. These results provide insights into flavour formation in S. pastorianus and illustrate the complexity of functional characterization in aneuploid strains.

  20. CBS på rekrutteringsjagt i USA

    DEFF Research Database (Denmark)

    Irwin, Alan

    2014-01-01

    Forskningsdekan Alan Irwin skriver i månedens klumme om CBS’ rekruttering af forsknings- og undervisningstalent.......Forskningsdekan Alan Irwin skriver i månedens klumme om CBS’ rekruttering af forsknings- og undervisningstalent....

  1. Structure of Debaryomyces castellii CBS 2923 phytase

    Science.gov (United States)

    Ragon, M.; Hoh, F.; Aumelas, A.; Chiche, L.; Moulin, G.; Boze, H.

    2009-01-01

    Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982 ▶). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 Å resolution. The crystals belonged to space group P6522, with unit-cell parameters a = 121.65, c = 332.24 Å. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R free = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules. PMID:19342770

  2. Covariant quantization of the CBS superparticle

    Energy Technology Data Exchange (ETDEWEB)

    Grassi, P.A. E-mail: pag5@nyu.edu; Policastro, G.; Porrati, M

    2001-07-09

    The quantization of the Casalbuoni-Brink-Schwarz superparticle is performed in an explicitly covariant way using the antibracket formalism. Since an infinite number of ghost fields are required, within a suitable off-shell twistor-like formalism, we are able to fix the gauge of each ghost sector without modifying the physical content of the theory. The computation reveals that the antibracket cohomology contains only the physical degrees of freedom.

  3. Covariant quantization of the CBS superparticle

    Science.gov (United States)

    Grassi, P. A.; Policastro, G.; Porrati, M.

    2001-07-01

    The quantization of the Casalbuoni-Brink-Schwarz superparticle is performed in an explicitly covariant way using the antibracket formalism. Since an infinite number of ghost fields are required, within a suitable off-shell twistor-like formalism, we are able to fix the gauge of each ghost sector without modifying the physical content of the theory. The computation reveals that the antibracket cohomology contains only the physical degrees of freedom.

  4. Structure of Debaryomyces castellii CBS 2923 phytase.

    Science.gov (United States)

    Ragon, M; Hoh, F; Aumelas, A; Chiche, L; Moulin, G; Boze, H

    2009-04-01

    Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.

  5. Rapid sample processing for intracellular metabolite studies in Penicillium ochrochloron CBS 123.824: the FiltRes-device combines cold filtration of methanol quenched biomass with resuspension in extraction solution.

    Science.gov (United States)

    Vrabl, Pamela; Artmann, Desiree J; Schinagl, Christoph W; Burgstaller, Wolfgang

    2016-01-01

    Many issues concerning sample processing for intracellular metabolite studies in filamentous fungi still need to be solved, e.g. how to reduce the contact time of the biomass to the quenching solution in order to minimize metabolite leakage. Since the required time to separate the biomass from the quenching solution determines the contact time, speeding up this step is thus of utmost interest. Recently, separation approaches based on cold-filtration were introduced as promising alternative to cold-centrifugation, which exhibit considerably reduced contact times. In previous works we were unable to obtain a compact pellet from cold methanol quenched samples of the filamentous fungus Penicillium ochrochloron CBS 123.824 via centrifugation. Therefore our aim was to establish for this organism a separation technique based on cold-filtration to determine intracellular levels of a selected set of nucleotides. We developed a cold-filtration based technique as part of our effort to revise the entire sample processing method and analytical procedure. The Filtration-Resuspension (FiltRes) device combined in a single apparatus (1) a rapid cold-filtration and (2) a rapid resuspension of the biomass in hot extraction solution. Unique to this is the injection of the extraction solution from below the membrane filter (FiltRes-principle). This caused the mycelial cake to detach completely from the filter membrane and to float upwards so that the biomass could easily be transferred into preheated tubes for metabolite extraction. The total contact time of glucose-limited chemostat mycelium to the quenching solution could be reduced to 15.7 ± 2.5 s, whereby each washing step added another 10-15 s. We evaluated critical steps like filtration time, temperature profile, reproducibility of results, and using the energy charge (EC) as a criterion, effectiveness of enzyme destruction during the transition in sample temperature from cold to hot. As control we used total broth samples

  6. A Response Surface Methodology Approach to Investigate the Effect of Sulfur Dioxide, pH, and Ethanol on DbCD and DbVPR Gene Expression and on the Volatile Phenol Production in Dekkera/Brettanomyces bruxellensis CBS2499

    Directory of Open Access Journals (Sweden)

    Federica Valdetara

    2017-09-01

    Full Text Available Dekkera/Brettanomyces bruxellensis, the main spoilage yeast in barrel-aged wine, metabolize hydroxycinnamic acids into off-flavors, namely ethylphenols. Recently, both the enzymes involved in this transformation, the cinnamate decarboxylase (DbCD and the vinylphenol reductase (DbVPR, have been identified. To counteract microbial proliferation in wine, sulfur dioxide (SO2 is used commonly to stabilize the final product, but limiting its use is advised to preserve human health and boost sustainability in winemaking. In the present study, the influence of SO2 was investigated in relation with pH and ethanol factors on the expression of DbCD and DbVPR genes and volatile phenol production in D. bruxellensis CBS2499 strain under different model wines throughout a response surface methodology (RSM. In order to ensure an exact quantification of DbCD and DbVPR expression, an appropriate housekeeping gene was sought among DbPDC, DbALD, DbEF, DbACT, and DbTUB genes by GeNorm and Normfinder algorithms. The latter gene showed the highest expression stability and it was chosen as the reference housekeeping gene in qPCR assays. Even though SO2 could not be commented as main factor because of its statistical irrelevance on the response of DbCD gene, linear interactions with pH and ethanol concurred to define a significant effect (p < 0.05 on its expression. The DbCD gene was generally downregulated respect to a permissive growth condition (0 mg/L mol. SO2, pH 4.5 and 5% v/v ethanol; the combination of the factor levels that maximizes its expression (0.83-fold change was calculated at 0.25 mg/L mol. SO2, pH 4.5 and 12.5% (v/v ethanol. On the contrary, DbVPR expression was not influenced by main factors or by their interactions; however, its expression is maximized (1.80-fold change at the same conditions calculated for DbCD gene. While no linear interaction between factors influenced the off-flavor synthesis, ethanol and pH produced a significant effect as

  7. Activation energies of pericyclic reactions: performance of DFT, MP2, and CBS-QB3 methods for the prediction of activation barriers and reaction energetics of 1,3-dipolar cycloadditions, and revised activation enthalpies for a standard set of hydrocarbon pericyclic reactions.

    Science.gov (United States)

    Ess, Daniel H; Houk, K N

    2005-10-27

    Activation barriers and reaction energetics for the three main classes of 1,3-dipolar cycloadditions, including nine different reactions, were evaluated with the MPW1K and B3LYP density functional methods, MP2, and the multicomponent CBS-QB3 method. The CBS-QB3 values were used as standards for 1,3-dipolar cycloaddition activation barriers and reaction energetics, and the density functional theory (DFT) and MP2 methods were benchmarked against these values. The MPW1K/6-31G* method and basis set performs best for activation barriers, with a mean absolute deviation (MAD) value of 1.1 kcal/mol. The B3LYP/6-31G* method and basis set performs best for reaction enthalpies, with a MAD value of 2.4 kcal/mol, while the MPW1K method shows large errors for reaction energetics. The MP2 method gives the expected systematic underestimation of barriers. Concerted and nearly synchronous transition structures are predicted by all DFT and MP2 methods. Also reported are revised estimated 0 K experimental activation enthalpies for a standard set of hydrocarbon pericyclic reactions and updated comparisons to experiment for DFT, ab initio, and multicomponent methods. B3LYP and MPW1K methods with MAD values of 1.5 and 2.1 kcal/mol, respectively, fortuitously outperform the multicomponent CBS-QB3 method, which has a MAD value of 2.3. The MAD value of the O3LYP functional improves to 2.4 kcal/mol from the previously reported 3.0 kcal/mol.

  8. A mathematical model for the study of lipid accumulation in oleaginous microorganisms. I. Lipid accumulation during growth of Mucor circinelloides CBS 172-27 on a vegetable oil

    Directory of Open Access Journals (Sweden)

    Aggelis, G.

    1995-06-01

    Full Text Available    The accumulation of lipids In microorganisms cultivated In growth media having as sole carbon and energy source vegetable or animal fat has been an object of research and industrial interest for many years. Interestingly, the accumulated fat often has a composition and structure much different from that of the fat present In the substrate.
       The present work describes a mathematical approach to the accumulation of fat by oleaginous microorganisms growing on medium containing vegetable oil as carbon source. A mathematical model, correlating the accumulation of reserve fat with the growth of microbial population and the available quantity of exocellular fat, is proposed. This model is verified by experimental data taken by cultivation of Mucor circinelloides CBS 172-27 on sunflower oil.
       The proposed model is described by the equation: XL = XLo + Lo(1-e-k2.t– (lnx-lnxo/k1    where XL(mg/l the concentration of reserve lipids at time t(h, XLo(mg/l the concentration of lipid reserves at time t=o, Lo(mg/l the initial concentration of exocellular fat (a t=o, X(mg/l the concentration of fat-free biomass at a given time t and Xo the concentration of fat-free biomass at time t=o; k1 and k2 constants.

       Durante muchos años la acumulación de lípidos en microorganismos desarrollados en medio de cultivo, tomando como única fuente de carbono y energía grasas vegetales o animales, ha sido objeto de investigación e Interés industrial.    Interesadamente, la grasa acumulada tiene a menudo una composición y estructura muy diferente de la que tiene la grasa presente en el sustrato.    El presente trabajo describe una aproximación matemática a la acumulaci

  9. Developing Islamic Banking Performance Measures Based on Maqasid Al-Shari’ah Framework: Cases of 24 Selected Banks

    Directory of Open Access Journals (Sweden)

    Mustafa Omar Mohammed

    2015-08-01

    Full Text Available Islamic banking has achieved remarkable growth that has surpassed the growth of the conventional banking system. Yet, studies show that the performances of Islamic banks (IBs seem to be trailing behind the conventional banks (CBs. Is the poor performance of IBs the result of mismatch between their objectives and their performance measurement criteria or it is a reflection of their true performances? The objectives of Islamic banking had not been formally addressed. Mustafa and Taib (2009 derived the objectives of Islamic banking from Abu Zahara’s theory of the objectives of Shari’ah (Maqasid al-Shari’ah and, based on these Shari’ah objectives, developed a model of Islamic banking performance measures. They named it as Performance Measures based on Maqasid al-Shari’ah framework or the PMMS model. This paper has tested the PMMS model on a sample 24 banks (12 IBs and 12 CBs. The twenty four banks were also evaluated using the traditional conventional financial measures. Mann-Whitney U-Test results show IBs faring well in their performances when measured using the PMMS model than when they are measured using the conventional banking performance yardstick. Policy prescriptions and recommendations for further studies are provided at the concluding section of the paper.

  10. A mixture of dioxins, furans, and non-ortho PCBs based upon consensus toxic equivalency factors produces dioxin-like reproductive effects.

    Science.gov (United States)

    Hamm, J T; Chen, C-Y; Birnbaum, L S

    2003-07-01

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; dioxin) and related polyhalogenated aromatic hydrocarbons (PHAHs) alter the reproductive development of laboratory animals. Therefore, we exposed animals to a mixture of dioxins, furans, and polychlorinated biphenyls (PCBs) that included TCDD, 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PeCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF), 1,2,3,7,8-pentachlorodibenzofuran (1-PeCDF), 2,3,4,7,8-pentachlorodibenzofuran (4-PeCDF), octachlorodibenzofuran (OCDF), 3,3',4,4'-tetrachlorobiphenyl (PCB77), 3,3',4,4',5-pentachlorobiphenyl (PCB126), and 3,3',4,4',5,5'-hexachlorobiphenyl (PCB169). The mixture composition approximated the relative abundance of these compounds in foodstuff (L. S. Birnbaum and M. J. DeVito, 1995, Toxicology Vol. 105, pp. 391-401). Following the work of Gray et al. with TCDD (1997, Toxicology and Applied Pharmacology Vol. 146, pp. 11-20), we exposed time-pregnant dams on gestation day (GD) 15 at doses up to 1.0 microgram TCDD toxic equivalency (TEQ)/kg and the development of offspring was monitored. This mixture significantly increased the time to puberty in both male and female offspring. At postnatal day (PND) 32 seminal vesicle weights were decreased; however, only ventral prostate weight was affected at PND 49 and no effects were seen at PND 63. In female offspring, the mixture caused dose-dependent increases in the incidence of vaginal thread. Ethoxyresorufin-O-deethylase (EROD) activity was higher than with TCDD the comparable TEQ exposure. Based on the slightly lowered responsiveness to the mixture, we used 2.0 microgram TEQ/kg to examine reproductive effects. This dose elicited the responses observed with 1.0 microgram TCDD/kg. Results indicate that the mixture causes a similar spectrum of effects seen with TCDD and the slightly lowered degree of response based on administered dose appears to be due to decreased transfer of mixture components to the offspring. Thus, the use of the WHO consensus TEFs (M. Van den Berg et al., 1998, Environ. Health Perspec. 106, 775-792) reasonably predicts the developmental toxicity of this mixture of dioxin-like PHAHs.

  11. Köyceğiz Gölü ve Dalyan Kanallarında Kıyı Kenar Çizgisinin UA ve CBS Teknikleri ile Belirlenmesi, Alanda Karşılaşılan Sorunlar

    Directory of Open Access Journals (Sweden)

    Serdar SELİM

    2016-06-01

    Full Text Available Köyceğiz-Dalyan Havzası, sahip olduğu doğal-kültürel varlık ve değerleri sebebiyle 1988 yılında ülkemizin ilk Özel Çevre Koruma Bölgesi (ÖÇKB olarak ilan edilmiştir. Yöre; su ve kıyı ekosistemini oluşturan Köyceğiz Gölü, Dalyan Kanalları, Dalyan Lagün Ağzı ve İztuzu kıyısı ile uluslararası ölçekte önem taşımaktadır. Ancak havzada kıyı kenar çizgisi henüz net bir şekilde ortaya konulmuş değildir. Bu durum kıyı kullanımlarında sorunlara ve kıyı alanları üzerine baskılara neden olmaktadır. Bu çalışmada, Köyceğiz Gölü ve Dalyan Kanallarında uzaktan algılama (UA ve coğrafi bilgi sistemleri (CBS kullanılarak, 3621 sayılı Kıyı Kanunu ile Kıyı Kanunun Uygulanmasına Dair Yönetmelik çerçevesinde kıyı kenar çizgisi tespit edilmiştir. Kıyı çizgisi ve kıyı kenar çizgisinin tespitinde, alana ait temel toprak haritalarının, ortofotoların ve güncel uydu verilerinin jeoreferanslama ve sayısallaştırma işlemleri gerçekleştirilmiş, haritalar çakıştırılmış ve arazi kontrolleri ile karşılaştırılmıştır. Sonuç olarak, herkesin eşit ve serbest olarak yararlanmasına açık olan Köyceğiz Gölü, Dalyan Kanalları ve İztuzu kıyısında ‘Kıyıda ve sahil şeridinde planlama ve uygulama yapılabilmesi için kıyı kenar çizgisinin tespiti zorunludur’ kanun hükmünün henüz uygulanmadığı görülmüştür. Yapılan analizlerde ulusal kıyı mevzuatına aykırı kullanımlar tespit edilmiştir. Uzaktan algılama ve coğrafi bilgi sistemleri teknikleriyle bölgede kıyı çizgisi ve kıyı kenar çizgisi tespit edilerek bu çalışmanın bölge kıyılarındaki planlama kararlarında belirleyici olması hedeflenmiştir. 

  12. PBL+CBS宣教模式在提升2型糖尿病患者治疗依从性的效果研究%The effect study of PBL + CBS health education missionary mode for enhancing the treatment compliance for patients with type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    余梅

    2014-01-01

    目的:探讨PBL+CBS宣教模式指导下的健康宣教对2型糖尿病患者治疗依从性的效果。方法选择2型糖尿病患者60例,随机分为观察组和对照组各30例,观察组采用PBL+CBS宣教模式的健康宣教,对照组采用常规宣教。比较两组患者干预后糖尿病知识掌握情况及住院期间患者的满意度。结果观察组患者的糖尿病知识掌握、合理饮食、运动锻炼、规律服药、定时检测血糖、戒烟戒酒的依从性评分及住院满意度得分均明显高于对照组(P<0.05)。结论 PBL+CBS宣教模式有利于提升2型糖尿病患者治疗依从性,值得推广和应用。%Objective To investigate the effect of PBL+CBS health education missionary mode for enhancing the treatment compliance for patients with type 2 diabetes. Methods 60 cases type 2 diabetic patients were randomly divided into observation group and control group of 30 cases, the observation group took health education PBL+CBS missionary mode, the control group took conventional missions mode. The diabetes knowledge level and patient satisfaction during hospitalization were compared between two groups.ResultsThe diabetes knowledge level, proper diet, exercise, regular medication, regular testing of blood glucose, tobacco and alcohol compliance score and hospital satisfaction scores of observation group were significantly higher than the control group(P<0.05). Conclusion PBL+CBS missionary mode can help enhance the treatment compliance for patients with type 2 diabetes, and it is worthy of promotion and application.

  13. CBS Kullanılarak Kıbrıs’ta Fırtına Riski Duyarlılık Analizi Storm Risk Sensitivity Analysis In Cyprus Using GIS

    Directory of Open Access Journals (Sweden)

    Emre ÖZŞAHİN

    2012-12-01

    ılda ortalama 2685 kişi etkilenirken, ekonomik olarak 14.340 milyon dolar zarar yaşanmıştır. Bu afetler içerisinde ise en büyük ekonomik zararı fırtınalar meydana getirmiştir. Yıllık ortalama 0.10 olarak yaşanan fırtınalardan çeşitli yıllarda 40 kişi etkilenirken, 10 milyon dolar ekonomik zarar yaşanmıştır.Bu çalışmada Kıbrıs’taki ülke ekonomilerine çok ciddi manada zarar veren fırtınalar, Coğrafi Bilgi Sistemleri (CBS yöntem ve tekniklerinden yararlanılarak meydana getirebileceği risk kapsamında analiz edilecektir. Acaba Kıbrıs adasında fırtına riskinin boyutları nasıldır? Adadaki hangi alanlar risk altındadır? Bu risk hangi yerleşmeleri en fazla etkileyecektir? Bu kapsamda yapılması gerekenler nelerdir? şeklindeki araştırma sorularına yanıtlar aranacaktır. Çalışmada değişik ölçeklerde ve farklı kaynaklardan elde edilen faktör haritalarından yararlanılmıştır. Bu haritalardan elde edilen jeoloji, jeomorfoloji, eğim, bakı, yağış, akarsuya uzaklık, yeraltı suyu, toprak ve arazi kullanım özelliklerine ait veriler koşullara bağlı ağırlıklı çakıştırma yöntemi ile birleştirilerek, taşkın risk alanları belirlenmiş ve haritalanmıştır.Çalışmanın zarar analizi sonuçlarına göre Kıbrıs’ta, geçmiş yıllarda yaşanmış, günümüz ve gelecekte yaşanabilecek fırtına değerleri geleceğe doğru 24.514.7 oranında artmaktadır. Fırtına risk analizi sonucuna göre ise adanın % 51.19’unun risk altında, % 27.51’ininde risk altında olmadığı anlaşılmıştır. Adada en riskli alanlar Lefkoşa yerleşim merkezi çevresidir. Riskin en az olduğu alanlar ise Girne ve Limasol çevreleri ile Trodos Dağları civarıdır. Bu kapsamda öncelikli olarak multidisipliner bir bilim anlayışıyla daha kapsamlı planlamaların yapılması ve halkın konu hakkında bilinçlendirilmesi gerekmektedir.

  14. Discovery of N-{4-[5-(4-Fluorophenyl)-3-methyl-2-methylsulfanyl-3H-imidazol-4-yl]-pyridin-2-yl}-acetamide (CBS-3595), a Dual p38α MAPK/PDE-4 Inhibitor with Activity against TNFα-Related Diseases.

    Science.gov (United States)

    Albrecht, Wolfgang; Unger, Anke; Bauer, Silke M; Laufer, Stefan A

    2017-07-13

    The anti-inflammatory potential of p38 mitogen-activated protein kinase (MAPK) inhibitors was coincidentally expanded to a dual inhibition of p38α MAPK and phosphodiesterase 4 (PDE4), and the potential benefits arising from the blockage of both inflammation-related enzymes were thoroughly investigated. The most promising compound, CBS-3595 (1), was successively evaluated in in vitro experiments as well as in ex vivo and in vivo preclinical studies after administration of 1 to rodents, dogs, and monkeys. The resulting data clearly indicated a potent suppression of tumor necrosis factor alpha release. For reconfirming the findings of the animal studies when administering 1 to healthy human volunteers, a phase I clinical trial was conducted. Apart from further information regarding the pharmacokinetic and pharmacodynamic characteristics of 1, it was demonstrated that dual inhibition of p38α MAPK and PDE4 is able to synergistically attenuate the excessive anti-inflammatory response.

  15. Towards measurement of political pressure on central banks in the emerging market economies: the case of the central bank of Egypt

    Directory of Open Access Journals (Sweden)

    Ibrahim L. Awad

    2008-05-01

    Full Text Available This paper assesses whether the legal independence granted to the Central Bank of Egypt (CBE under the latest legislation is factual. I followed Fry’s methodology, which assumes that the level of independence of the central bank is determined by fiscal attributes. In an attempt to develop Fry’s method, I used a simple criterion to assess the central bank’s independence, namely, that the central bank is actually independent if it can fulfill its money supply target. Applying this criterion to the CBE and some other CBs in the developed countries and emerging market economies, we find that: (i the legal independence granted to the CBE under the latest legislation is not factual; although the final objective of monetary policy is to achieve price stability, the CBE failed to fulfill its money supply target and achieve price stability, because it was responsive to political pressure and did not react to fulfill its money supply target; (ii such political pressure on the CBE is due to fiscal attributes, as measured by domestic credit to the government; (iii CBs whose independence is factual, according to our criterion, showed a negative relationship between the legal indices, as measured by the GMT index, and the fiscal attributes measured by DCGY. However, the relationship was anomalous when measured by the rate of inflation

  16. 孕期饮用酒精对子代大鼠学习记忆及海马H2S/CBS系统的影响%Effect of alcohol exposure during pregnancy on learning and memory and H2S/CBS in the hippocampus of infant rats

    Institute of Scientific and Technical Information of China (English)

    徐春阳; 杜爱林; 姜洪波; 张艳; 郝伟; 张瑞岭

    2012-01-01

    Objective To observe the effect of alcohol exposure during pregnancy on learning and memory and content of hydrogen sulfide(H2S) in the hippocampus of infant rats.Methods The animal models of alcohol exposure during pregnancy were made,and the learning and memory were evaluated by Y-maze in adult offspring.Content of H2S and activity of cystathionine-beta-synthase(GBS) in the hippocampus of the brain were evaluated with spectrophotometry;and CBS protein expression in the hippocampus was detected by immunohistochemistry.Results The learning and memory ( (43.00 ± 15.33 ) times) of alcohol exposure during pregnancy group was significantly decreased compared with that of control and drinking groups (( 25.13 ± 12.35 )times and (26.12 ±11.95 ) times,P < 0.05 ) ; spectrophotometry results showed that the content of H2S ( ( 30.32 ± 5.84 ) nmoL/g) of alcohol exposure during pregnancy group was significantly increased compared with that of control ( ( 52.51 ±7.85 ) nmol/g) and drinking groups( (49.93 ± 4.29 ) nmol/g),and the activity of CBS( ( 55.13 ± 4.45 ) nmol/g)of alcohol exposure during pregnancy group was significantly increased (P < 0.01 ) compared with that of control ( (71.06 ± 5.58 ) nmol/g) and drinking groups( (69.96 ± 6.13 ) nmol/g) ; immunohistochemistry showed that the expression of CBS protein of alcohol exposure during pregnancy group was significantly increased.Conclusion The damage effect of alcohol exposure during pregnancy on nerve system of infant rats may interrelate with down-regulation of H2S/CBS in the hippocampus.%目的 研究孕期饮用酒精对子代大鼠学习记忆及海马硫化氢(H2S)的影响.方法 建立大鼠孕期饮用酒精模型,子代成年后,检测学习记忆、海马组织H2S含量、胱硫醚β-合酶(cystathionine-betasynthase,CBS)活性,免疫组化检测海马区CBS蛋白表达.结果 学习记忆测试结果显示孕期饮用酒精组子鼠学习记忆成绩[(43.00±15.33)次]比正常对照组

  17. Köyyollarının Takibi, Bakım Ve Onarımı İçin Coğrafi Bilgi Sistemi (CBS Esaslı Yönetim Modeli: Denizli Örneği

    Directory of Open Access Journals (Sweden)

    Yetiş Şazi Murat

    2013-06-01

    Full Text Available Coğrafi Bilgi Sistemleri (CBS özellikle gelişmiş ülkelerde her alanda sıklıkla kullanılan bir araç haline gelmişken Türkiye' de, merkezi ve yerel yönetimlerce benimsenmiş olmakla birlikte kamu hizmetlerinde kullanımı ise henüz emekleme aşamasındadır. Denizli İl Özel İdaresi Yol ve Ulaşım Hizmetleri Müdürlüğü bünyesinde il sınırları içindeki sorumluluk ve hizmet alanında bulunan köyyolları envanterinin güncellenmesi, farklı analiz sonuçları üzerinden Müdürlüğün kırsal altyapı hizmetlerine modern ve teknik yöntemlerle yön verilmesi amaçlı CBS destekli bir çalışma ortaya konulmuştur. Strateji Geliştirme Müdürlüğü ile birlikte yürütülen bu çalışmada farklı amaçlar için geliştirilmiş olan katmanlar, topoğrafik haritalar, uydu fotoğrafları vb. araçlardan istifade edilerek köyyolları ile ilgili yürütülen hizmetin doğru ve güncel verilere dayalı olmasına çalışılmıştır. Kamu kaynaklarının etkin kullanılmasını sağlayabilecek hassas ve hızlı analiz sonuçlarına göre köyyolları ulaşımının yeniden planlanması, eksik ve hatalı durumların tespiti ile tam ve bütünleşik bir yönetim sistemi kurulması amaçlı pilot bir proje ortaya konulmaya ve ürün olarak kullanılması yoluna gidilmiştir.

  18. What quantum measurements measure

    Science.gov (United States)

    Griffiths, Robert B.

    2017-09-01

    A solution to the second measurement problem, determining what prior microscopic properties can be inferred from measurement outcomes ("pointer positions"), is worked out for projective and generalized (POVM) measurements, using consistent histories. The result supports the idea that equipment properly designed and calibrated reveals the properties it was designed to measure. Applications include Einstein's hemisphere and Wheeler's delayed choice paradoxes, and a method for analyzing weak measurements without recourse to weak values. Quantum measurements are noncontextual in the original sense employed by Bell and Mermin: if [A ,B ]=[A ,C ]=0 ,[B ,C ]≠0 , the outcome of an A measurement does not depend on whether it is measured with B or with C . An application to Bohm's model of the Einstein-Podolsky-Rosen situation suggests that a faulty understanding of quantum measurements is at the root of this paradox.

  19. Measuring unrecorded measurement

    Science.gov (United States)

    Revzen, M.; Mann, A.

    2016-08-01

    Projective (von Neumann) measurement of an operator (i.e., a dynamical variable) selected from a prescribed set of operators is termed unrecorded measurement (URM) when both the selected operator and the measurement outcome are unknown, i.e., “lost”. Within classical physics a URM is completely inconsequential: the state is unaffected by measurement. Within quantum physics a measurement leaves a mark. The present study provides protocols that allow the retrieval of some of the data lost in a URM. The study is shown as supportive of viewing quantum measurement as made up of both classical-like and pure quantum components.

  20. Measures for Measures

    DEFF Research Database (Denmark)

    Jørgensen, Sune Lehman; A.D., Jackson; Lautrup, B.E.

    2006-01-01

    Are some ways of measuring scientific quality better than others? Sune Lehmann, Andrew D. Jackson and Benny E. Lautrup analyse the reliability of commonly used methods for comparing citation records....

  1. Body measurements and slaughter parameters in four close bred stocks of japanese quail reared under different levels of dietary lysine

    Directory of Open Access Journals (Sweden)

    S. Ahmad

    2013-05-01

    Full Text Available The aim of study was to evaluate body measurements and slaughter parameters in four close bred stock (CBS of Japanese quail (named as Major (M, Kaleem (K, Saadat (S and Zahid (Z reared under different levels of dietary lysine at Avian Research and Training (ART Centre, UVAS Lahore, Pakistan. For this purpose 1440 broiler quail chick were procured form ART Centre hatchery, birds were reared at different levels of dietary lysine  i.e. 1.3% lysine for 28 days (Phase-I, 1.4% lysine for 1st 14 days and 1.2% lysine for last 14 o (Phase-II, 1.5% lysine for 9 days, 1.3% lysine for next 10 days and 1.1% lysine for last 9 days (Phase-III. A total of 108 birds {4(CBS×3(Phases×9 birds}, were slaughtered at the age of 27 weeks and their body measurements and slaughter parameters were recorded. The data thus obtained were statistically analyzed through Randomized Complete Block Design (RCBD. The means were separated out through Duncan’s Multiple Range test using SAS 9.1 for windows. Statistical analysis revealed significant differences (P < 0.05 among different CBS and Phases. In body measurements body, shank and drumstick length and drumstick circumference showed significant difference (P<0.05 while wing spread, keel length and shank circumferences showed non-significant difference. In slaughter parameters carcass, heart and gizzard weight, and intestinal length showed significant differences (P<0.05 whereas body, liver and intestinal weight showed non-significant differences.

  2. Full CI Benchmark Potentials for the 6e^- System Li_2 with a CBS Extrapolation from aug-cc-pCV5Z and aug-cc-pCV6Z Basis Sets Using Fciqmc and Dmrg

    Science.gov (United States)

    Dattani, Nikesh S.; Sharma, Sandeep; Alavi, Ali

    2016-06-01

    Being the simplest uncharged homonuclear dimer after H_2 that has a stable ground state, Li_2 is one of the most important benchmark systems for theory and experiment. In 1930, Delbruck used Li_2 to test his theory of homopolar binding, and it was used again and again as a prototype to test what have now become some of the most ubiquitous concepts in molecular physics (LCAO, SCF, MO, just to name a few). Experimentally, Roscoe and Schuster studied alkali dimers back in 1874. At the dawn of quantum mechanics, the emerging types of spectroscopic analyses we now use today, were tested on Li_2 in the labs of Wurm (1928), Harvey (1929), Lewis (1931), and many others, independently. Li_2 was at the centre of the development of PFOODR in the 80s, and PAS in the 90s; and Lithium Bose-Einstein condensates were announced only 1 month after the Nobel Prize winning BEC announcement in 1995. Even now in the 2010s, numerous experimental and theoretical studies on Li have tested QED up to the 7th power of the fine structure constant. Li_2 has also been of interest to sub-atomic physicists, as it was spectroscopic measurements on ^7Li_2 that determined the spin of ^7Li to be 3/2 in 1931; and Li_2 has been proposed in 2014 as a candidate for the first ``halo nucleonic molecule". The lowest triplet state a(1^3Σ_u^+) is an excellent benchmark system for all newly emerging ab initio techniques because it has only 6e^-, its potential is only 334 cm-1 deep, it avoids harsh complications from spin-orbit coupling, and it is the deepest potential for which all predicted vibrational energy levels have been observed with 0.0001 cm-1 precision. However the current best ab initio potentials do not even yield all vibrational energy spacings correct to within 1 cm-1. This could be because the calculation was only done on a cc-pV5Z basis set, or because the QCISD(T,full) method that the authors used, only considered triple excitations while a full CI calculation should include up to hexuple

  3. Measure for measure

    Science.gov (United States)

    Weinberger, Peter

    2012-06-01

    The present system of measures for length, weight and capacity (volume) originates from scientific ideas expressed during the French Revolution in 1789. The history of a compatible unit of length, however, turns out to be less of a scientific but rather of a political character. Here reports to the Philosophical Magazine made in the first quarter of the nineteenth century are used to trace the cultural split between meters and inches, and between kilograms and pounds, that can be experienced in many parts of the world.

  4. CBS-institutleder: Der er masser af muligheder i blockchain

    DEFF Research Database (Denmark)

    Damsgaard, Jan

    2017-01-01

    Flere virksomheder fokuserer på blockchain, ligesom flere iværksættere og uddannelsesinstitutioner også gør. Derfor er der masser af muligheder i blockchain, der venter på at blive taget i brug......Flere virksomheder fokuserer på blockchain, ligesom flere iværksættere og uddannelsesinstitutioner også gør. Derfor er der masser af muligheder i blockchain, der venter på at blive taget i brug...

  5. Case report 476: Chondrosarcoma of base of skull (CBS)

    Energy Technology Data Exchange (ETDEWEB)

    Boorstein, J.M.; Spizarny, D.L.

    1988-04-01

    In summary, focal neurologic findings in a 29-year-old woman were secondary to a mass at the skull base. Skull roentgenograms and CT localized a partially calcified, non-enhancing, left parasellar mass, which was partially resected. Two years later the patient presented with similar symptoms and locally recurrent mass. Oseochondroma was originally considered; however, tumor recurrence, radiographic manifestations, and morphologic features were felt more consistent with chondrosarcoma. This patient illustrates that diagnosis of low grade chondrosarcoma requires cooperation between skeletal radiologist, skeletal pathologist and surgeon. (orig./SHA).

  6. CBS domain-containing proteins are Rhizopus oryzae ferrioxamine receptors

    Science.gov (United States)

    Background: Iron-overload patients treated with deferoxamine are uniquely susceptible to mucormycosis, because Rhizopus spp. can obtain iron from ferrioxamine (deferoxamine + Fe**3+). Previously we have identified two closely related, ferrioxamine-inducible R. oryzae genes (FOB1 and FOB2) in which ...

  7. 棘阿米巴土壤分离株CB/S1的线粒体DNA限制性片段长度多态性%MITOCHONDRIAL DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) OF ACANTHAMOEBA SP. CB/S1 ISOLAT ED FROM SOIL

    Institute of Scientific and Technical Information of China (English)

    崔春权; 玄英花; 郑善子; 金松竹; 杨香

    2012-01-01

    [目的]观察棘阿米巴土壤分离株CB/S1的线粒体DNA限制性片段长度多态性(RFLP).[方法]从棘阿米巴CB/S1提取线粒体DNA用Eco RI酶切,与土壤分离株CJY/S4比较观察限制性片段长度多态性.用地衣红-卡红染色观察棘阿米巴内共生细菌.[结果]棘阿米巴土壤分离株CB/S1线粒体DNA RFLP与CJY/S4相比除了额外的片段(extra bands),两个分离株具有相同的片段模式.经地衣红染色观察CB/S1内共生细菌呈黑色、棒状、不规则的分布在胞质内.[结论]额外的片段表明棘阿米巴CB/S1内含有内共生细菌的环状DNA,并且除了内共生细菌的存在棘阿米巴CB/S1与土壤分离株CJY/S4具有密切的亲缘关系.线粒体DNA限制性片段长度多态性是棘阿米巴分类及发现内共生细菌的简便而有效的方法.%[Objective] To survey characterization of Mitochondrial DNA Restriction Fragment Length Polymorphism (MtDNA RFLP) of Aeanthamoeba sp. CB/SI isolated from soil of china. [Methods] The extracted MlDNA was digested with the Eco RI restriction enzyme, electrophoresed in 0.7% agarose gel. The endosymbiont of Acanthamoeba sp. was characterized with orcein-camiime staining [Results] Acanthamoeba sp. CB/SI showED extra bands as weli as common bands with the other Aeanthamoeba sp. CJY/S4 strain isolated from soil, rode-shaped endosymbionts were randomly distributed within the cytoplasm of trophozoite of Aeanthamoeba sp. CB/SI. [Conclusion] These results indicated that the.Acanlhamoeba sp. CB/SI may possi-bly harbor intracellular endosymbiotic bacteria containing circular DNAs. With the exception of the presence of endosymbionts, Aeanthamoeba sp. CB/S1 with Aeanthamoeba CJY/S4 were found to be closely phylogenetically related.

  8. Respiratory capacities of mitochondria of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 grown under glucose limitation

    NARCIS (Netherlands)

    Urk, Hendrik van; Bruinenberg, Peter M.; Veenhuis, Marten; Scheffers, W. Alexander; Dijken, Johannes P. van

    1989-01-01

    A comparative study was made of the in vitro respiratory capacity of mitochondria isolated from Saccharomyces cerevisiae and Candida utilis grown in glucose-limited chemostat cultures. An electron-microscopic analysis of whole cells revealed that the volume density of mitochondria was the same in

  9. Concentrations of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in blood collected from 195 pregnant women in Sapporo City, Japan.

    Science.gov (United States)

    Todaka, Takashi; Hirakawa, Hironori; Kajiwara, Jumboku; Hori, Tsuguhide; Tobiishi, Kazuhiro; Onozuka, Daisuke; Kato, Shizue; Sasaki, Seiko; Nakajima, Sonomi; Saijo, Yasuaki; Sata, Fumihiro; Kishi, Reiko; Iida, Takao; Furue, Masutaka

    2007-10-01

    We measured the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), non-ortho coplanar polychlorinated biphenyls (non-ortho PCBs), and mono-ortho coplanar polychlorinated biphenyls (mono-ortho PCBs) in blood collected between 2002 and 2004 from 195 pregnant women living in Sapporo City of Hokkaido Prefecture, Japan. The present study is one of the few studies in which PCDDs, PCDFs, and dioxin-like PCBs have been measured in the blood of pregnant women. Of the 195 pregnant women, 101 were primipara (mean: 28.8 years) and 94 were multipara (mean: 32.4 years). The arithmetic mean total TEQ concentrations of PCDDs, PCDFs, non-ortho PCBs, and mono-ortho PCBs of primiparous and multiparous mothers in Sapporo City were 17.4 (median: 15.7) and 14.0 (median: 13.9)pgTEQ/glipid, respectively, and the concentrations were in the range of 6.6-43.4 and 3.4-28.2pgTEQ/glipid, respectively. In regard to the relationship between the total TEQ concentrations of PCDDs, PCDFs, non-ortho PCBs, and mono-ortho PCBs in maternal blood and the number of deliveries or age of primiparous and multiparius mothers, the levels of these dioxin-like compounds tended to decrease with increases in the number of deliveries and significantly increased with increasing maternal age in both groups. Furthermore, significant correlations were observed between the total TEQ concentrations of these dioxin-like compounds and the age of primiparae and multiparae. The total TEQ concentration of PCDDs, PCDFs, non-ortho PCBs, and mono-ortho PCBs measured in 195 pregnant women has decreased compared to past levels in Japan for the last several decades. The results of the present study have indicated that dioxin contamination in the blood of young women in Japan is continuing to decrease.

  10. s Measurements

    Indian Academy of Sciences (India)

    Sunanda Banerjee

    2003-11-01

    The strong coupling constant, s, has been determined from many pure inclusive and semi-inclusive measurements. All these measurements, measured at different scales, are consistent among each other and the measurements can be combined to give s()=0.118 ± 0.003.

  11. Measuring Savings

    OpenAIRE

    Mark Schreiner

    2001-01-01

    Development depends on saving. But what exactly is saving, and how is it measured? This paper defines saving and describes several measures of financial savings. The measures account for the passage of time and for the three stages of saving: putting in (depositing), keeping in (maintaining a balance), and taking out (withdrawing). Together, the different measures capture how people move financial resources through time.

  12. Measuring Happiness

    OpenAIRE

    Richter, Luise; Unger, Cindie

    2015-01-01

    The project at hand aims at outlining if and how happiness measurements can constitute an alternative to the neoliberal measurements of prosperity dominating today. For this purpose, four cases, namely the UN 2013 World Happiness Report and the OECD 2013 Guidelines on measuring subjective well-being as one entity, the Happy Planet Index 2012 from the New Economics Foundation and the report on the Bhutanese Gross National Happiness measurements (2012), have been selected. These perspectives, w...

  13. Measure Lines

    Science.gov (United States)

    Crissman, Sally

    2011-01-01

    One tool for enhancing students' work with data in the science classroom is the measure line. As a coteacher and curriculum developer for The Inquiry Project, the author has seen how measure lines--a number line in which the numbers refer to units of measure--help students not only represent data but also analyze it in ways that generate…

  14. Measurement evaluation

    CERN Document Server

    Boros, A

    1990-01-01

    The information obtained about a measured object is called ``crude'' measurement information and must be related to the conditions under which the measurement took place. Using ``crude'' measurement information as a starting point, evaluation produces physically correctly interpreted data with their estimated (or corrected) error. Although a number of works deal with the evaluation of measurements, they either appeared a long time ago or serve essentially different aims. This book gives a comprehensive and current overview on the basic principles, aids, devices, and methods in the eval

  15. Quantum measurement

    CERN Document Server

    Busch, Paul; Pellonpää, Juha-Pekka; Ylinen, Kari

    2016-01-01

    This is a book about the Hilbert space formulation of quantum mechanics and its measurement theory. It contains a synopsis of what became of the Mathematical Foundations of Quantum Mechanics since von Neumann’s classic treatise with this title. Fundamental non-classical features of quantum mechanics—indeterminacy and incompatibility of observables, unavoidable measurement disturbance, entanglement, nonlocality—are explicated and analysed using the tools of operational quantum theory. The book is divided into four parts: 1. Mathematics provides a systematic exposition of the Hilbert space and operator theoretic tools and relevant measure and integration theory leading to the Naimark and Stinespring dilation theorems; 2. Elements develops the basic concepts of quantum mechanics and measurement theory with a focus on the notion of approximate joint measurability; 3. Realisations offers in-depth studies of the fundamental observables of quantum mechanics and some of their measurement implementations; and 4....

  16. Field Measurements

    CERN Document Server

    Bottura, L

    2004-01-01

    The measurement of the magnetic field is often the final verification of the complex design and fabrication process of a magnetic system. In several cases, when seeking high accuracy, the measurement technique and its realization can result in a considerable effort. This note describes most used measurement techniques, such as nuclear magnetic resonance, fluxmeters and Hall generators, and their typical range of application. In addition some of less commonly used techniques, such as magneto-optical, SQUIDs, or particle beams methods, are listed.

  17. Load Measurements

    DEFF Research Database (Denmark)

    Vesth, Allan; Kock, Carsten Weber

    The report describes Load measurements carried out on a given wind turbine. The aim of the measurement program regarding the loads on the turbine is to verify the basic characteristics of the wind turbine and loads on the blades, the rotor and the tower, using [Ref 1], [Ref2] and [Ref 3]. Regardi...

  18. Load Measurements

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    The report describes Load measurements carried out on a given wind turbine. The aim of the measurement program regarding the loads on the turbine is to verify the basic characteristics of the wind turbine and loads on the blades, the rotor and the tower, using [Ref 1], [Ref2] and [Ref 3]. Regarding...

  19. Measuring Colour

    CERN Document Server

    Hunt, R W G

    2011-01-01

    The classic authority on colour measurement now fully revised and updated with the latest CIE recommendations The measurement of colour is of major importance in many commercial applications, such as the textile, paint, and foodstuff industries; as well as having a significant role in the lighting, paper, printing, cosmetic, plastics, glass, chemical, photographic, television, transport, and communication industries. Building upon the success of earlier editions, the 4th edition of Measuring Colour has been updated throughout with new chapters on colour rendering by light sources; colorimetry

  20. Mechanical measurements

    CERN Document Server

    Venkateshan, S P

    2015-01-01

    The first edition of this book was co-published by Ane Books India, and CRC Press in 2008. This second edition is an enlarged version of the web course developed by the author at IIT Madras, and also a modified and augmented version of the earlier book.  Major additions/modifications presented are in the treatment of errors in measurement, temperature measurement, measurement of thermo-physical properties, and data manipulation. Many new worked examples have been introduced in this new and updated second edition. 

  1. Diversity Measures

    Directory of Open Access Journals (Sweden)

    MSc. Mentor Ademaj

    2012-06-01

    Full Text Available Diversity measures are a type of non-criminal measures foreseen in the Chapter IV of the Code of Juvenile Justice, which may be imposed on juvenile perpetrators of criminal acts. These measures can be applied in cases of minor offenses, for which is foreseen the criminal sanction with a fine or imprisonment up to three years or for criminal offenses committed by negligence for which is foreseen the sentence up to five years of imprisonment, except those cases that result in death. With the imposition of these measures is intended to prevent criminal proceedings against juveniles whenever is possible, rehabilitation and reintegration of juvenile in his/her community and the prevention of recidivist behaviour. Competent authority to impose them is the public prosecutor, the juvenile judge and juvenile court. And they are executed by the Kosovo Correctional Service.

  2. Horizon measures

    KAUST Repository

    Zhang, Eugene

    2016-11-28

    In this paper we seek to answer the following question: where do contour lines and visible contour lines (silhouette) tend to occur in a 3D surface. Our study leads to two novel shape descriptors, the horizon measure and the visible horizon measure, which we apply to the visualization of 3D shapes including archeological artifacts. In addition to introducing the shape descriptors, we also provide a closed-form formula for the horizon measure based on classical spherical geometry. To compute the visible horizon measure, which depends on the exact computation of the surface visibility function, we instead of provide an image-based approach which can process a model with high complexity within a few minutes.

  3. Interoperability Measurement

    Science.gov (United States)

    2008-08-01

    measurement methods (non-maturity model-based methods in italics, maturity-based methods in boldface) .................... 11  Figure 3. LISI ...OIM LISI , IAM QoIM SoIM Figure 2. Chronology of published interoperability measurement methods (non-maturity model- based methods in italics... method called Levels of Information System Interoperability ( LISI ) (Figure 3). (Ibid) This method was eventually formalized and mandated in CJCSI

  4. Measuring needs

    Directory of Open Access Journals (Sweden)

    Laura Murianni

    2008-06-01

    Full Text Available The interest in measuring the health of populations, through measuring the demand and offers of health care, is deeply rooted in history. Population health indicators in use today are built upon from mortality measures from the 1500s; regular census information starting from the 1800s; civil registration records of vital statistics commencing in the 1850s; regular national surveys, which were first initiated in the 1950s; and health system and other administrative databases used widely since the 1960s. The ancient Greeks believed that the God of medicine had two daughters: Hygeia and Panacea, the first was the goddess of prevention and wellness, while the second was the goddess of treatment. Thus suggesting that people have long believed that there is more to health than health care. Today the actual concept of population health recognizes many interconnected aspects of society, the environment, and individuals all contributing to health. To increase opportunities for comparability, more valid, comprehensive and standardized ways of measuring and reporting on population health indicators are needed. The use of health indicators contributes to overall population health goals, namely improving the health populations, reducing health inequalities and measuring the performance of health care system. The objective of performance assessment is to provide governments and populations with information about the state of their health care system.

  5. When Measurement Benefits the Measured

    Science.gov (United States)

    2014-04-23

    5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Carnegie Mellon University ,Software Engineering Institute,Pittsburgh,PA...15213 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR...versionone.com/assets/ img /files/CHAOSManifesto2013.pdf 4 When Measurement Benefits the Measured Kasunic & Nichols, April 23, 2014 © 2014 Carnegie Mellon

  6. Measurement uncertainty.

    Science.gov (United States)

    Bartley, David; Lidén, Göran

    2008-08-01

    The reporting of measurement uncertainty has recently undergone a major harmonization whereby characteristics of a measurement method obtained during establishment and application are combined componentwise. For example, the sometimes-pesky systematic error is included. A bias component of uncertainty can be often easily established as the uncertainty in the bias. However, beyond simply arriving at a value for uncertainty, meaning to this uncertainty if needed can sometimes be developed in terms of prediction confidence in uncertainty-based intervals covering what is to be measured. To this end, a link between concepts of accuracy and uncertainty is established through a simple yet accurate approximation to a random variable known as the non-central Student's t-distribution. Without a measureless and perpetual uncertainty, the drama of human life would be destroyed. Winston Churchill.

  7. Microwave Measurements

    CERN Document Server

    Skinner, A D

    2007-01-01

    The IET has organised training courses on microwave measurements since 1983, at which experts have lectured on modern developments. Their lecture notes were first published in book form in 1985 and then again in 1989, and they have proved popular for many years with a readership beyond those who attended the courses. The purpose of this third edition of the lecture notes is to bring the latest techniques in microwave measurements to this wider audience. The book begins with a survey of the theory of current microwave circuits and continues with a description of the techniques for the measureme

  8. SAFETY MEASURES

    CERN Multimedia

    Relations with the Host States Service; Tel. 75152

    2001-01-01

    Following the recent terrorist attacks, the French authorities have introduced increased-vigilance measures («Vigipirate renforcé») as part of their prevention of terrorism campaign and have expressed the wish to extend these measures to the French part of the CERN site. The Organization has acceded to this request with the understanding that its international status will be respectd and has granted the French Gendarmerie right of access to the Prévessin site and to the LEP/LHC sites on French territory.

  9. Load Measurements

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    The report describes Load measurements carried out on a given wind turbine. The aim of the measurement program regarding the loads on the turbine is to verify the basic characteristics of the wind turbine and loads on the blades, the rotor and the tower, using [Ref 1], [Ref2] and [Ref 3]. Regarding...... the fatigue loads, the rotor, blades and tower moments are presented. The fatigue loads are evaluated using rainflow counting described in detail in Ref. [1]. The 1Hz equivalent load ranges are calculated at different wind speeds. All information regarding the instrumentation is collected in [ref 4] and [ref...

  10. Measuring Up

    Science.gov (United States)

    Hart, Holly; Healey, Kaleen; Sporte, Susan E.

    2014-01-01

    Moving teacher evaluation systems from measuring teachers' performance to improving their practice requires much greater attention to communication and support. In the fall of 2012, Chicago Public Schools (CPS) instituted a sweeping reform of its teacher evaluation system with the introduction of REACH Students (Recognizing Educators Advancing…

  11. Happiness measures

    NARCIS (Netherlands)

    R. Veenhoven (Ruut)

    2014-01-01

    markdownabstract__Abstract__ The word “happiness” is used for different meanings, and these meanings all require different measures. This lemma is about happiness in the sense of subjective enjoyment of one’s life as a whole that is also called “life satisfaction.” Two “components” of

  12. Measuring Resilience.

    Science.gov (United States)

    Hoffman, Robert R; Hancock, P A

    2017-06-01

    As human factors and ergonomics (HF/E) moves to embrace a greater systems perspective concerning human-machine technologies, new and emergent properties, such as resilience, have arisen. Our objective here is to promote discussion as to how to measure this latter, complex phenomenon. Resilience is now a much-referenced goal for technology and work system design. It subsumes the new movement of resilience engineering. As part of a broader systems approach to HF/E, this concept requires both a definitive specification and an associated measurement methodology. Such an effort epitomizes our present work. Using rational analytic and synthetic methods, we offer an approach to the measurement of resilience capacity. We explicate how our proposed approach can be employed to compare resilience across multiple systems and domains, and emphasize avenues for its future development and validation. Emerging concerns for the promise and potential of resilience and associated concepts, such as adaptability, are highlighted. Arguments skeptical of these emerging dimensions must be met with quantitative answers; we advance one approach here. Robust and validated measures of resilience will enable coherent and rational discussions of complex emergent properties in macrocognitive system science.

  13. Measurement of \

    CERN Document Server

    Aguilar-Arevalo, A A; Bazarko, A O; Brice, S J; Brown, B C; Bugel, L; Cao, J; Coney, L; Conrad, J M; Cox, D C; Curioni, A; Djurcic, Z; Finley, D A; Fleming, B T; Ford, R; Garcia, F G; Garvey, G T; Gonzales, J; Grange, J; Green, C; Green, J A; Hart, T L; Hawker, E; Imlay, R; Johnson, R A; Karagiorgi, G; Kasper, P; Katori, T; Kobilarcik, T; Kourbanis, I; Koutsoliotas, S; Laird, E M; Linden, S K; Link, J M; Liu, Y; Louis, W C; Mahn, K B M; Marsh, W; Mauger, C; McGary, V T; McGregor, G; Metcalf, W; Meyers, P D; Mills, F; Mills, G B; Monroe, J; Moore, C D; Mousseau, J; Nelson, R H; Nienaber, P; Nowak, J A; Osmanov, B; Ouedraogo, S; Patterson, R B; Pavlovic, Z; Perevalov, D; Polly, C C; Prebys, E; Raaf, J L; Ray, H; Roe, B P; Russell, A D; Sandberg, V; Schirato, R; Schmitz, D; Shaevitz, M H; Shoemaker, F C; Smith, D; Soderberg, M; Sorel, M; Spentzouris, P; Spitz, J; Stancu, I; Stefanski, R J; Sung, M; Tanaka, H A; Tayloe, R; Tzanov, M; Van de Water, R G; Wascko, M O; White, D H; Wilking, M J; Yang, H J; Zeller, G P; Zimmerman, E D

    2009-01-01

    MiniBooNE reports the first absolute cross sections for neutral current single \\pi^0 production on CH_2 induced by neutrino and antineutrino interactions measured from the largest sets of NC \\pi^0 events collected to date. The principal result consists of differential cross sections measured as functions of \\pi^0 momentum and \\pi^0 angle averaged over the neutrino flux at MiniBooNE. We find total cross sections of (4.76+/-0.05_{stat}+/-0.40_{sys})*10^{-40} cm^2/nucleon at a mean energy of =808 MeV and (1.48+/-0.05_{stat}+/-0.14_{sys})*10^{-40} cm^2/nucleon at a mean energy of =664 MeV for \

  14. Measurement of \

    Energy Technology Data Exchange (ETDEWEB)

    Aguilar-Arevalo, A.A.; /Mexico U., ICN; Anderson, C.E.; /Yale U.; Bazarko, A.O.; /Princeton U.; Brice, S.J.; /Fermilab; Brown, B.C.; /Fermilab; Bugel, L.; /Columbia U.; Cao, J.; /Michigan U.; Coney, L.; /Columbia U.; Conrad, J.M.; /MIT; Cox, D.C.; /Indiana U.; Curioni, A.; /Yale U. /Columbia U.

    2009-11-01

    MiniBooNE reports the first absolute cross sections for neutral current single {pi}{sup 0} production on CH{sub 2} induced by neutrino and antineutrino interactions measured from the largest sets of NC {pi}{sup 0} events collected to date. The principal result consists of differential cross sections measured as functions of {pi}{sup 0} momentum and {pi}{sup 0} angle averaged over the neutrino flux at MiniBooNE. We find total cross sections of (4.76 {+-} 0.05{sub stat} {+-} 0.40{sub sys}) x 10{sup -40} cm{sup 2}/nucleon at a mean energy of = 808 MeV and (1.48 {+-} 0.05{sub stat} {+-} 0.14{sub sys}) x 10{sup -40} cm{sup 2}/nucleon at a mean energy of = 664 MeV for {nu}{sub {mu}} and {bar {nu}}{sub {mu}} induced production, respectively. In addition, we have included measurements of the neutrino and antineutrino total cross sections for incoherent exclusive NC 1{pi}{sup 0} production corrected for the effects of final state interactions to compare to prior results.

  15. Antenna Measurement

    OpenAIRE

    Picard, Dominique

    2010-01-01

    Currently it is possible to measure all the characteristics of an antenna with a good accuracy. Far-field ranges do not have a very good accuracy, due to parasitic reflections for the outdoor ranges and because of the limited distance between the source antenna and the tested antenna for the indoor ranges. The compact range allows one to obtain a direct farfield cut in a relatively short time. The near-field techniques are the most accurate and the most convenient for global antenna radiation...

  16. Measuring Fractality

    Directory of Open Access Journals (Sweden)

    Tatjana eStadnitski

    2012-05-01

    Full Text Available When investigating fractal phenomena, the following questions are fundamental for the applied researcher: (1 What are essential statistical properties of 1/f noise? (2 Which estimators are available for measuring fractality? (3 Which measurement instruments are appropriate and how are they applied? The purpose of this article is to give clear and comprehensible answers to these questions. First, theoretical characteristics of a fractal pattern (self-similarity, long memory, power law and the related fractal parameters (the Hurst coefficient, the scaling exponent, the fractional differencing parameter d of the ARFIMA methodology, the power exponent of the spectral analysis are discussed. Then, estimators of fractal parameters from different software packages commonly used by applied researchers (R, SAS, SPSS are introduced and evaluated. Advantages, disadvantages, and constrains of the popular estimators are illustrated by elaborate examples. Finally, crucial steps of fractal analysis (plotting time series data, autocorrelation and spectral functions; performing stationarity tests; choosing an adequate estimator; estimating fractal parameters; distinguishing fractal processes from short memory patterns are demonstrated with empirical time series.

  17. Measuring fractality.

    Science.gov (United States)

    Stadnitski, Tatjana

    2012-01-01

    WHEN INVESTIGATING FRACTAL PHENOMENA, THE FOLLOWING QUESTIONS ARE FUNDAMENTAL FOR THE APPLIED RESEARCHER: (1) What are essential statistical properties of 1/f noise? (2) Which estimators are available for measuring fractality? (3) Which measurement instruments are appropriate and how are they applied? The purpose of this article is to give clear and comprehensible answers to these questions. First, theoretical characteristics of a fractal pattern (self-similarity, long memory, power law) and the related fractal parameters (the Hurst coefficient, the scaling exponent α, the fractional differencing parameter d of the autoregressive fractionally integrated moving average methodology, the power exponent β of the spectral analysis) are discussed. Then, estimators of fractal parameters from different software packages commonly used by applied researchers (R, SAS, SPSS) are introduced and evaluated. Advantages, disadvantages, and constrains of the popular estimators ([Formula: see text] power spectral density, detrended fluctuation analysis, signal summation conversion) are illustrated by elaborate examples. Finally, crucial steps of fractal analysis (plotting time series data, autocorrelation, and spectral functions; performing stationarity tests; choosing an adequate estimator; estimating fractal parameters; distinguishing fractal processes from short-memory patterns) are demonstrated with empirical time series.

  18. Multipath Measurements

    Science.gov (United States)

    1974-08-01

    positioned to the elevation desired for the measure- men , sequences and reflectec pulse amplitude levels recorded as a function of time as discussed...31 NRNOBINT(XNR*IG) YNBNS«NRN6 1F(YWHN0,LT,XNBNG)MH»JC»’’»NG«1 * 1F(RFS0LTN.U1 .» HVP »Hf2nN^NC»NCC 9 GO TO «oOO 821 00 850 J»l,NBE»MS CALL...RRHOARYflO) COMMON/TC*INP/GIR(«),G2P(«) CUMMnN/TC*!N0/r.2nPf*O,Gl0P(«) CüMMON/OERV/P*RH*V(l?7),* HVP »Hf5)»CBETZ0(l0),C»0.G20(J0)iNP» 1 OIROOH.DFLT

  19. 210Pb and 137Cs measurements in the Circum Bohai Sea coastal region: sedimentation rates and implications

    Institute of Scientific and Technical Information of China (English)

    Fu WANG; Hong WANG; Jianfen LI; Yandong PEI; Changfu FAN; Lizhu TIAN; Zhiwen SHANG; Meiyu SONG; Yan GENG

    2008-01-01

    The representative 210Pb and 137Cs age-depth profiles were selected from more than 100 sites in the Circum Bohai Sea (CBS)coastal region to examine the sedimentation rates and associated environmental changes.There are three unique 210Pbexc distribution patterns:1)ideal-decaying type,representing relatively constant sediment supply and quieter environmental setting;2)wiggling type,but with approximately equivalent amplitude,representing coarser sediments and associated stronger flow current environment;and 3) episodic-eventinfluencing type,each representing typhoon storm-influenced depositional environment.Our results also show that there is a lower sedimentation rate(ca.0.1 cm/a)in the coastal lowlands,a higher sedimentation rate(ca.0.53 cm/a)in the intertidal flat and an intermediate rate of ca.0.58 cm/a in the subtidal zone near the Huanghua Harbor.The interbedded silt layer occurring in the core sediment reveals lower 210Pb activities,indicating a discontinuous sedimentation mostly due to typhoon events which dwarfs against 210Pb and 137Cs applicability.Overall,the 210Pb and 137Cs measurements of the present study provide physical insight into the evaluation of the coastal-marine ecological environment and associated management.

  20. Energy saving by improvement in heat storage tank. Complex building `Sun Pedeck` management by CBS; Chikunetsuso kairyonado de shoene. Sogo tatemono `Sun Pedeck` (kabushiki kaisha CBS)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    An example in which energy was considerably saved by improving the underground heat storage tank in complex building `Sun Pedeck` in front of Tsudanuma Station is introduced as the interview of an excellent business. Many speciality shops and restaurants move into this complex building, and the building consists of three buildings containing offices and cultural facilities. Therefore, the complicated management that the complex building is in the open state throughout the year and variously limited in equipment replacement is required. Since a cold-water tank of 450 tons under the ground is an initial heat storage tank, cold water is accumulated only at the bottom. This was very low in efficiency. A weir was thus installed in the cool-water tank to improve so that the water flow meanders up and down. At 10:00 p.m. to 8:00 a.m. that is the midnight time zone of power, a turbo-refrigerating machine for regenerative heat storage operates between 7:00 and 8:00 in the early morning so as to produce cool water. The annual power rate could be reduced 6,000,000 yen by circulating the heat-reserved cool water between 2:30 p.m. and 3:30 p.m. 6 figs.

  1. Measurements and Terminology of Different Measure Methods

    Institute of Scientific and Technical Information of China (English)

    FANG Fang; ZHANG Wei-yuan; ZHANG Wen-bin

    2005-01-01

    Body measuring is very important for garment sizing and pattern making. In this paper, we study the difference of the landmarks between the traditional method and 3D scanner and we also select the 19 circumference measurements,29 height and length measurements, 18 breadth and depth measurements and 3 other measurements, which are quite important in fashion body measuring, to compare the terminology of them in these two measuring method. 3D scanners seem better than the traditional method on these aspects, which are the number of measurements, speed,privacy and data accuracy, but they are limited on measuring posture. And there is no uniform standard for the scanners and the definitions of the measurements in the scanners are diversified.

  2. Time change, jumping measure and Feller measure

    OpenAIRE

    He, Ping

    2007-01-01

    In this paper, we shall investigate some potential theory for time change of Markov processes. Under weak duality, it is proved that the jumping measure and Feller measure are actually independent of time change, and the jumping measure of a time changed process induced by a PCAF supported on $V$ coincides with the sum of the Feller measure on $V$ and the trace of the original jumping measure on $V$.

  3. Positive motivic measures are counting measures

    CERN Document Server

    Ellenberg, Jordan S

    2009-01-01

    Let K be a field. A positive motivic measure on the Grothendieck ring K_0(Var_K) is a homomorphism from K_0(Var_K) to the real numbers assigning a nonnegative value to every variety. In this note we show that the only positive motivic measures are the counting measures: measures on K_0(Var_{F_q}) which send a variety to its number of rational points over some fixed finite extension of F_q.

  4. Work measurement as a generalized quantum measurement.

    Science.gov (United States)

    Roncaglia, Augusto J; Cerisola, Federico; Paz, Juan Pablo

    2014-12-19

    We present a new method to measure the work w performed on a driven quantum system and to sample its probability distribution P(w). The method is based on a simple fact that remained unnoticed until now: Work on a quantum system can be measured by performing a generalized quantum measurement at a single time. Such measurement, which technically speaking is denoted as a positive operator valued measure reduces to an ordinary projective measurement on an enlarged system. This observation not only demystifies work measurement but also suggests a new quantum algorithm to efficiently sample the distribution P(w). This can be used, in combination with fluctuation theorems, to estimate free energies of quantum states on a quantum computer.

  5. A designated centre for people with disabilities operated by Brothers of Charity Services Galway - Galway

    LENUS (Irish Health Repository)

    Krijt, Jakub

    2011-02-01

    Cystathionine β-synthase (CBS) deficiency is usually confirmed by assaying the enzyme activity in cultured skin fibroblasts. We investigated whether CBS is present in human plasma and whether determination of its activity in plasma could be used for diagnostic purposes. We developed an assay to measure CBS activity in 20 μL of plasma using a stable isotope substrate - 2,3,3-(2)H serine. The activity was determined by measurement of the product of enzyme reaction, 3,3-(2)H-cystathionine, using LC-MS\\/MS. The median enzyme activity in control plasma samples was 404 nmol\\/h\\/L (range 66-1,066; n = 57). In pyridoxine nonresponsive CBS deficient patients, the median plasma activity was 0 nmol\\/ho\\/L (range 0-9; n = 26), while in pyridoxine responsive patients the median activity was 16 nmol\\/hour\\/L (range 0-358; n = 28); this overlapped with the enzyme activity from control subject. The presence of CBS in human plasma was confirmed by an in silico search of the proteome database, and was further evidenced by the activation of CBS by S-adenosyl-L-methionine and pyridoxal 5\\'-phosphate, and by configuration of the detected reaction product, 3,3-(2)H-cystathionine, which was in agreement with the previously observed CBS reaction mechanism. We hypothesize that the CBS enzyme in plasma originates from liver cells, as the plasma CBS activities in patients with elevated liver aminotransferase activities were more than 30-fold increased. In this study, we have demonstrated that CBS is present in human plasma and that its catalytic activity is detectable by LC-MS\\/MS. CBS assay in human plasma brings new possibilities in the diagnosis of pyridoxine nonresponsive CBS deficiency.

  6. MEASURE FOR MEASURE: A THRICE TOLD TALE

    Directory of Open Access Journals (Sweden)

    HALIMAH MOHAMED ALI

    2009-01-01

    Full Text Available This paper argues that Measure for Measure is a difficult play to perform because it has problematic themes, especially the theme of sexuality, that clash with the way of life and thinking of contemporary society. As such, any director who chooses to stage it must consider these difficulties and how to present them in a natural manner without making the audience feel that the whole production is contrived. The directors of the two major productions discussed in this paper tried their best to present a Measure for Measure that would be acceptable to the modern society. It is evident that there are many interpretations of the Duke and Isabella's characters and also of Isabella's reaction to the Duke's proposal at the end of the play. It can be concluded that no interpretation is wrong because each actor or director brings with him his own reading of the play, and every reading has been influenced by other performances and textual criticisms. Since Measure for Measure is a thematically rich play, it should not be confined to a single interpretation. The different performances of Measure for Measure have proven that theatre is experimental as well as ageless. Because it is a brilliant play with a myriad of interpretations, Measure for Measure will not cease to be a favourite for directors in times to come. It is not wrong to predict that fans of Shakespeare in general and of Measure for Measure in particular can look forward to many more productions.For experienced Shakespeare observers all performances are thrice-told tales. The perceiving eye absorbs the performance even as the mind's eye attends to the text. Both are augmented by the inner ear buzzing with those other voices, both critical and dramatic, carried by the observer into the theatre.(Crowl, Samuel 1992: 3

  7. Measurements on Hearing

    DEFF Research Database (Denmark)

    Poulsen, Torben

    1996-01-01

    Background material for measurements of hearing for grammar school pupils. The note gives the necessary background for the exercise 'Measurement on Hearing'. The topics comprise sound and decibel, the ear, basic psychoacoustics, hearing threshold, audiometric measurement methods, speech and speech...

  8. Measuring Test Measurement Error: A General Approach

    Science.gov (United States)

    Boyd, Donald; Lankford, Hamilton; Loeb, Susanna; Wyckoff, James

    2013-01-01

    Test-based accountability as well as value-added asessments and much experimental and quasi-experimental research in education rely on achievement tests to measure student skills and knowledge. Yet, we know little regarding fundamental properties of these tests, an important example being the extent of measurement error and its implications for…

  9. Semiconductor Electrical Measurements Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Semiconductor Electrical Measurements Laboratory is a research laboratory which complements the Optical Measurements Laboratory. The laboratory provides for Hall...

  10. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

    DEFF Research Database (Denmark)

    Pel, Herman J.; de Winde, Johannes H.; Archer, David B.

    2007-01-01

    of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli...

  11. Duration of University Studies - The Case of cand.merc. EAF at CBS

    DEFF Research Database (Denmark)

    Milhøj, Anders; laCour, Lisbeth

    2014-01-01

    In this paper the duration of studying the master program Applied Economics and Finance at Copenhagen Business School is studied. The data applied are all students beginning the program summer 2010. The length of their study time is modeled using various independent variables such as age, gender ...

  12. Velkommen til 2015 på CBS - Velkommen til et år med udfordringer

    DEFF Research Database (Denmark)

    Molin, Jan

    2015-01-01

    I denne måneds klumme fra Direktionen skriver uddannelsesdekan Jan Molin om det første af en række paradokser, han vil beskrive i løbet af året i sine klummer. Månedens klumme drejer sig om paradokset "kontrol er en forudsætning for tillid" og nødvendigheden af at opgive illusionen om kontrol....

  13. Statistical estimates and physics of close binary systems (CBS) of neutron stars.

    Science.gov (United States)

    Callegari, G.; Bregola, M.; Ferrario, C.; Passerini, A.

    After some statistical considerations about the number of CBSs with one neutron star in our galaxy, the authors study the possible kinds of emission of CBSs with two neutron stars. They give considerations on the dimension of such a system at the moment of its formation. From the comparison of different kinds of power emitted they deduce that the orbital gravitational emission Pgo dominates. The dynamical evolution of the orbital elements a and e is determined by Pgo and by tidal friction, which produce opposite effects. The quantitative comparison between Pgo and Ptf (work made per unit time by tidal torque) says that Pgo > Ptf. Therefore the authors conclude that a < 0 and e < 0; that is the evolution of the systems is towards collapse.

  14. Draft Genome Sequence of the Ant-Associated Fungus Phialophora attae (CBS 131958).

    Science.gov (United States)

    Moreno, Leandro F; Stielow, J Benjamin; de Vries, Michel; Weiss, Vinicius A; Vicente, Vania A; de Hoog, Sybren

    2015-11-19

    The black yeast Phialophora attae was isolated from the cuticle of tropical ant gynes. The ant-fungus association is sustained due to symbiotic evolutionary adaptations that allow fungal assimilation and tolerance of toxic compounds produced by the ant. The genome sequence of the first ant-associated fungus, P. attae, is presented here. Copyright © 2015 Moreno et al.

  15. Draft Genome Sequence of the Mycoparasitic Oomycete Pythium periplocum Strain CBS 532.74.

    Science.gov (United States)

    Kushwaha, Sandeep K; Vetukuri, Ramesh R; Grenville-Briggs, Laura J

    2017-03-23

    The oomycete Pythium periplocum is an aggressive mycoparasite of a number of plant pathogenic fungi and oomycetes and therefore has potential as a biological control agent. Here, we report the first draft genome sequence of P. periplocum, which comprises 35.89 Mb. It contains 1,043 scaffolds and 14,399 predicted protein-coding genes. Copyright © 2017 Kushwaha et al.

  16. The CBS spectra investigation as method of the PN chemical composition analysis

    Science.gov (United States)

    Shimanskaya, N. N.; Bikmaev, I. F.; Shimansky, V. V.; Sakhibullin, N. A.; Zhuchkov, R. Ya.; Shigapov, R. R.

    2007-10-01

    We report the results of the investigations of chemical composition of close binaries which had gone through the stage of common envelope and which are the remnants of planetary nebular cores. High resolution spectra for different phases of orbital period of V471 Tau were taken by RTT-150 telescope and were investigated by the modified SYNTH program. The spectra show noticeable variability with an appearance of emission components dependent of the orbital period phase. For chemical composition determination, the "solar" oscillator strengths of 700 lines were determined. It was found that the chemical content of V471 Tau is a composite one and is characterized by excess of α-process elements in the contrast to small underabundance of iron-peak elements. An estimation of different element abundances in the star allows to determine their contents in planetary nebular phase.

  17. Draft Genome Sequence of Fonsecaea nubica Strain CBS 269.64, Causative Agent of Human Chromoblastomycosis.

    Science.gov (United States)

    Costa, Flávia F; de Hoog, Sybren; Raittz, Roberto T; Weiss, Vinicius A; Leão, Aniele C R; Bombassaro, Amanda; Sun, Jiufeng; Moreno, Leandro F; Souza, Emanuel M; Pedrosa, Fabio O; Steffens, Maria Berenice R; Baura, Valter; Tadra-Sfeir, Michele Z; Balsanelli, Eduardo; Najafzadeh, M Javad; Gomes, Renata R; Felipe, Maria S; Teixeira, Marcus; Santos, Germana D; Xi, Liyan; Alves de Castro, Mauro Antônio; Vicente, Vânia A

    2016-08-04

    On the basis of multilocus phylogenetic data, Fonsecaea nubica was described in 2010 as a molecular sibling of F. monophora, an established agent of the human skin disease chomoblastomycosis in tropical zones. Genome analysis of these pathogens is mandatory to identify genes involved in the interaction with host and virulence. Copyright © 2016 Costa et al.

  18. Draft Genome Sequence of the Ant-associated Fungus Phialophora attae (CBS 131958)

    NARCIS (Netherlands)

    de Hoog, S.

    2015-01-01

    The black yeast Phialophora attae was isolated from the cuticle of tropical ant gynes. The ant-fungus association is sustained due to symbiotic evolutionary adaptations that allow fungal assimilation and tolerance of toxic compounds produced by the ant. The genome sequence of the first ant-associate

  19. Towards implementing CLIL (Content and Language Integrated Learning at CBS (Tunja, Colombia

    Directory of Open Access Journals (Sweden)

    Claudia Marina Mariño

    2014-09-01

    Full Text Available Lately, the use of CLIL in classrooms has been expanding due to the interest in educating bilingual children. In Colombia, this approach is becoming known and is being applied in many schools, mainly bilingual. This article presents a case study research that sought to investigate how CLIL is applied at Country Bilingual School to contribute to the bilingual education offered to these students. The study was supported by the latest theoretical constructs about CLIL from representative authors such as Coyle, Hood and Marsh (2010, among others. The data consisted of six videotaped classes from fifth grade, a class observation form, a students’ questionnaire, an informal interview, a teacher’s journal and other documents, such as the Teacher’s lesson plans. The results were qualitatively and quantitatively analyzed using Seliger and Shohamy (1990 techniques. The study revealed that, although the observed classes meet most of the standards of a good class in terms of language teaching methodology, there are many aspects that need to be considered and applied in order to use CLIL effectively.

  20. Complete hydrolysis of myo-inositol hexakisphosphate by a novel phytase from Debaryomyces castellii CBS 2923.

    Science.gov (United States)

    Ragon, Mélanie; Aumelas, André; Chemardin, Patrick; Galvez, Santiago; Moulin, Guy; Boze, Hélène

    2008-02-01

    Debaryomyces castellii phytase was purified to homogeneity in a single step by hydrophobic interaction chromatography. Its molecular mass is 74 kDa with 28.8% glycosylation. Its activity was optimal at 60 degrees C and pH 4.0. The K (m) value for sodium phytate was 0.532 mM. The enzyme exhibited a low specificity and hydrolyzed many phosphate esters. The phytase fully hydrolyzed myo-inositol hexakisphosphate (or phytic acid, Ins P(6)) to inositol and inorganic phosphate. The sequence of Ins P(6) hydrolysis was determined by combining results from high-performance ionic chromatography and nuclear magnetic resonance. D. castellii phytase is a 3-phytase that sequentially releases phosphate groups through Ins (1,2,4,5,6) P(5), Ins (1,2,5,6) P(4), Ins (1,2,6) P(3), Ins (1,2) P(2), Ins (1 or 2) P(1), and inositol (notation 3/4/5/6/1 or 2).

  1. Are the Weak Measurements Really Measurements?

    Directory of Open Access Journals (Sweden)

    Dmitri Sokolovski

    2013-05-01

    Full Text Available Weak measurements can be seen as an attempt at answering the Which way? question without destroying interference between the pathways involved. Unusual mean values obtained in such measurements represent the response of a quantum system to this forbidden question, in which the true composition of virtual pathways is hidden from the observer. Such values indicate a failure of a measurement where the uncertainty principle says it must fail, rather than provide an additional insight into physical reality.Quanta 2013; 2: 50–57.

  2. QCD measurements in ATLAS

    CERN Document Server

    Llorente Merino, Javier; The ATLAS collaboration

    2017-01-01

    Several measurements in jet and photon physics are presented. The jet physics results include measurements of jet cross sections as well as various measurements on jet substructure and the precise determination of the strong coupling constant. Photon results include the measurements of several processes involving photons (inclusive, diphoton, photon + jet)

  3. Completely random signed measures

    DEFF Research Database (Denmark)

    Hellmund, Gunnar

    Completely random signed measures are defined, characterized and related to Lévy random measures and Lévy bases.......Completely random signed measures are defined, characterized and related to Lévy random measures and Lévy bases....

  4. Can Virtue Be Measured?

    Science.gov (United States)

    Curren, Randall; Kotzee, Ben

    2014-01-01

    This article explores some general considerations bearing on the question of whether virtue can be measured. What is moral virtue? What are measurement and evaluation, and what do they presuppose about the nature of what is measured or evaluated? What are the prospective contexts of, and purposes for, measuring or evaluating virtue, and how would…

  5. Measuring agricultural policy bias

    DEFF Research Database (Denmark)

    Jensen, Henning Tarp; Robinson, Sherman; Tarp, Finn

    2010-01-01

    Measurement is a key issue in the literature on price incentive bias induced by trade policy. We introduce a general equilibrium measure of the relative effective rate of protection, which generalizes earlier protection measures. For our fifteen sample countries, results indicate that the agricul......Measurement is a key issue in the literature on price incentive bias induced by trade policy. We introduce a general equilibrium measure of the relative effective rate of protection, which generalizes earlier protection measures. For our fifteen sample countries, results indicate...... protection measure is therefore uniquely suited to capture the full impact of trade policies on relative agricultural price incentives....

  6. Evaluating Measurement Accuracy

    CERN Document Server

    Rabinovich, Semyon G

    2010-01-01

    The goal of Evaluating Measurement Accuracy: A Practical Approach is to present methods for estimating the accuracy of measurements performed in industry, trade, and scientific research. Although multiple measurements are the focus of current theory, single measurements are the ones most commonly used. This book answers fundamental questions not addressed by present theory, such as how to discover the complete uncertainty of a measurement result. In developing a general theory of processing experimental data, this book, for the first time, presents the postulates of the theory of measurements. It introduces several new terms and definitions about the relationship between the accuracy of measuring instruments and measurements utilizing these instruments. It also offers well-grounded and practical methods for combining the components of measurement inaccuracy. From developing the theory of indirect measurements to proposing new methods of reduction in place of the traditional ones, this work encompasses the ful...

  7. How to Measure the Quantum Measure

    CERN Document Server

    Frauca, Álvaro Mozota

    2016-01-01

    The histories-based framework of Quantum Measure Theory assigns a generalized probability or measure $\\mu(E)$ to every (suitably regular) set $E$ of histories. Even though $\\mu(E)$ cannot in general be interpreted as the expectation value of a selfadjoint operator (or POVM), we describe an arrangement which makes it possible to determine $\\mu(E)$ experimentally for any desired $E$. Taking, for simplicity, the system in question to be a particle passing through a series of Stern-Gerlach devices or beam-splitters, we show how to couple a set of ancillas to it, and then to perform on them a suitable unitary transformation followed by a final measurement, such that the probability of a final outcome of "yes" is related to $\\mu(E)$ by a known factor of proportionality. Finally, we discuss in what sense a positive outcome of the final measurement should count as a minimally disturbing verification that the microscopic event $E$ actually happened.

  8. Finding generically stable measures

    CERN Document Server

    Simon, Pierre

    2010-01-01

    We discuss two constructions for obtaining generically stable Keisler measures in an NIP theory. First, we show how to symmetrize an arbitrary invariant measure to obtain a generically stable one from it. Next, we show that suitable sigma-additive probability measures give rise to generically stable measures. Also included is a proof that generically stable measures over o-minimal theories and the p-adics are smooth.

  9. Reactive and regulative temperament in patients with compulsive buying and non-clinical controls measured by self-report and performance-based tasks.

    Science.gov (United States)

    Voth, Eva M; Claes, Laurence; Georgiadou, Ekaterini; Selle, Janine; Trotzke, Patrick; Brand, Matthias; de Zwaan, Martina; Müller, Astrid

    2014-10-01

    To examine reactive and regulative temperament in patients with compulsive buying (CB) by means of self-report measures and performance-based tasks and to explore the relationship between both measurement approaches. The study included 31 treatment-seeking patients with CB (25 women, 6 men) and an age and gender matched non-clinical control group without CB (CG). All participants answered the Compulsive Buying Scale (CBS). Reactive temperament was assessed using the Behavioral Inhibition System/Behavioral Activation System Scales (BIS/BAS) and the Iowa Gambling Task (IGT). Regulative temperament was measured using the Effortful Control subscale of the Adult Temperament Questionnaire (ATQ-EC) and a computerized version of the Stroop Task. To control the results for depression, the Patient Health Questionnaire-Depression Scale (PHQ-9) was administered. Crude group comparisons revealed higher BIS and BAS scores, poorer IGT performance and lower ATQ-EC scores in the CB-group compared to the CG. The groups did not differ in their performance on the Stroop task. After controlling for depressive symptoms that were significantly higher in the CB-group, only the group differences in BAS reactivity remained significant. No significant associations were found between questionnaires and performance-based tasks. Overall, the findings indicate that CB in the present clinical sample of treatment-seeking patients was mainly associated with higher approach tendencies and more depressive symptoms. The lacking correlation between self-reports and performance-based tasks is in line with prior research and suggests that both methodologies tap into different aspects of temperament. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Temperature Measurements in the Magnetic Measurement Facility

    Energy Technology Data Exchange (ETDEWEB)

    Wolf, Zachary

    2010-12-13

    Several key LCLS undulator parameter values depend strongly on temperature primarily because of the permanent magnet material the undulators are constructed with. The undulators will be tuned to have specific parameter values in the Magnetic Measurement Facility (MMF). Consequently, it is necessary for the temperature of the MMF to remain fairly constant. Requirements on undulator temperature have been established. When in use, the undulator temperature will be in the range 20.0 {+-} 0.2 C. In the MMF, the undulator tuning will be done at 20.0 {+-} 0.1 C. For special studies, the MMF temperature set point can be changed to a value between 18 C and 23 C with stability of {+-}0.1 C. In order to ensure that the MMF temperature requirements are met, the MMF must have a system to measure temperatures. The accuracy of the MMF temperature measurement system must be better than the {+-}0.1 C undulator tuning temperature tolerance, and is taken to be {+-}0.01 C. The temperature measurement system for the MMF is under construction. It is similar to a prototype system we built two years ago in the Sector 10 alignment lab at SLAC. At that time, our goal was to measure the lab temperature to {+-}0.1 C. The system has worked well for two years and has maintained its accuracy. For the MMF system, we propose better sensors and a more extensive calibration program to achieve the factor of 10 increase in accuracy. In this note we describe the measurement system under construction. We motivate our choice of system components and give an overview of the system. Most of the software for the system has been written and will be discussed. We discuss error sources in temperature measurements and show how these errors have been dealt with. The calibration system is described in detail. All the LCLS undulators must be tuned in the Magnetic Measurement Facility at the same temperature to within {+-}0.1 C. In order to ensure this, we are building a system to measure the temperature of the

  11. Power Curve Measurements REWS

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Vesth, Allan

    This report describes the power curve measurements carried out on a given wind turbine in a chosen period. The measurements were carried out following the measurement procedure in the draft of IEC 61400-12-1 Ed.2 [1], with some deviations mostly regarding uncertainty calculation. Here, the refere......This report describes the power curve measurements carried out on a given wind turbine in a chosen period. The measurements were carried out following the measurement procedure in the draft of IEC 61400-12-1 Ed.2 [1], with some deviations mostly regarding uncertainty calculation. Here......, the reference wind speed used in the power curve is the equivalent wind speed obtained from lidar measurements at several heights between lower and upper blade tip, in combination with a hub height meteorological mast. The measurements have been performed using DTU’s measurement equipment, the analysis...

  12. Quality measurement in healthcare.

    Science.gov (United States)

    Lazar, Eliot J; Fleischut, Peter; Regan, Brian K

    2013-01-01

    Measurement is the basis for assessing potential improvements in healthcare quality. Measures may be classified into four categories: volume, structure, outcome, and process (VSOP). Measures of each type should be used with a full understanding of their cost and benefit. Although volume and structure measures are easily collected, impact on healthcare results is not always clear. Process measures are generally more difficult and expensive to collect, and the relationship between process and outcomes is only recently being explored. Knowledge of measure types and relationships among them, as well as emerging evidence on the role of patient satisfaction, must be used to guide improvements and ultimately for demonstrating value in healthcare.

  13. Power Curve Measurements REWS

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Villanueva, Héctor

    This report describes the power curve measurements carried out on a given wind turbine in a chosen period. The measurements were carried out following the measurement procedure in the draft of IEC 61400-12-1 Ed.2 [1], with some deviations mostly regarding uncertainty calculation. Here......, the reference wind speed used in the power curve is the equivalent wind speed obtained from lidar measurements at several heights between lower and upper blade tip, in combination with a hub height meteorological mast. The measurements have been performed using DTU’s measurement equipment, the analysis...

  14. Split-second recognition: what makes outdoor advertising work?

    NARCIS (Netherlands)

    van Meurs, A.; Aristoff, M.

    2009-01-01

    CBS Outdoor used a tachistoscope to determine how long it takes to recognize the brand/product advertised in 187 outdoor posters in the Netherlands. Additionally, CBS Outdoor measured the creative appeal of these advertisements. Using 80 content and format variables, an explanatory model was develop

  15. Split-second recognition: what makes outdoor advertising work?

    NARCIS (Netherlands)

    van Meurs, A.; Aristoff, M.

    2009-01-01

    CBS Outdoor used a tachistoscope to determine how long it takes to recognize the brand/product advertised in 187 outdoor posters in the Netherlands. Additionally, CBS Outdoor measured the creative appeal of these advertisements. Using 80 content and format variables, an explanatory model was

  16. MDS Quality Measures

    Data.gov (United States)

    U.S. Department of Health & Human Services — A list of the quality measures displayed on Nursing Home Compare, excluding Measures of Rehospitalization, Emergency Room Visit, and Community Discharge. Each row...

  17. Global Climate Measurement Project

    Data.gov (United States)

    National Aeronautics and Space Administration — When investigating the processes in the atmosphere, measurement of trace gas concentrations is essential.  However, measuring trace gas concentrations is...

  18. Measuring Physical Activity Intensity

    Medline Plus

    Full Text Available ... Older Adults Overcoming Barriers Measuring Physical Activity Intensity Target Heart Rate & Estimated Maximum Heart Rate Perceived Exertion ( ... a heavy backpack Other Methods of Measuring Intensity Target Heart Rate and Estimated Maximum Heart Rate Perceived ...

  19. Structural Measures - Hospital

    Data.gov (United States)

    U.S. Department of Health & Human Services — A list of hospitals and the availability of structural measures at that hospital. A structural measure reflects the environment in which hospitals care for patients....

  20. Developing Effective Performance Measures

    Science.gov (United States)

    2014-10-14

    University When Performance Measurement Goes Bad Laziness Vanity Narcissism Too Many Pettiness Inanity 52 Developing Effective...Kasunic, October 14, 2014 © 2014 Carnegie Mellon University Narcissism Measuring performance from the organization’s point of view, rather than from

  1. Ethernet traffic measurement

    OpenAIRE

    Santschi, Yann; Corthay, François

    2012-01-01

    The goal of this project is to create a FPGA Ethernet traffic measurement point and interface it with an existing measurement network, named DPMI, that is developed in Blekinge Institute of Technology.

  2. Emissions & Measurements - Black Carbon

    Science.gov (United States)

    Emissions and Measurement (EM) research activities performed within the National Risk Management Research Lab NRMRL) of EPA's Office of Research and Development (ORD) support measurement and laboratory analysis approaches to accurately characterize source emissions, and near sour...

  3. Conductivity Measurements of Silverpastes

    Directory of Open Access Journals (Sweden)

    M. Dirix

    2010-01-01

    Full Text Available The development of three-dimensional printed circuit boards requires research on new materials which can easily be deformed. Conducting pastes are well suited for deformation even after they are applied to the dielectric carrier. This paper deals with measurements of the electrical conductivity of these conducting pastes. Two different conductivity measurement techniques are explained and carried out. The resulting measurements give an overview of the conductivity of several measured samples.

  4. Foundations of measurement

    CERN Document Server

    Suppes, Patrick

    1989-01-01

    Foundations of Measurement offers the most coherently organized treatment of the topics and issues central to measurement. Much of the research involved has been scattered over several decades and a multitude of journals--available in many instances only to specialties. With the publication of Volumes two and three of this important work, Foundations of Measurement is the most comprehensive presentation in the area of measurement.

  5. Software engineering measurement

    CERN Document Server

    Munson, PhD, John C

    2003-01-01

    By demonstrating how to develop simple experiments for the empirical validation of theoretical research and showing how to convert measurement data into meaningful and valuable information, this text fosters more precise use of software measurement in the computer science and software engineering literature. Software Engineering Measurement shows you how to convert your measurement data to valuable information that can be used immediately for software process improvement.

  6. Spectrum and network measurements

    CERN Document Server

    Witte, Robert A

    2014-01-01

    This new edition of Spectrum and Network Measurements enables readers to understand the basic theory, relate it to measured results, and apply it when creating new designs. This comprehensive treatment of frequency domain measurements successfully consolidates all the pertinent theory into one text. It covers the theory and practice of spectrum and network measurements in electronic systems. It also provides thorough coverage of Fourier analysis, transmission lines, intermodulation distortion, signal-to-noise ratio and S-parameters.

  7. Top quark measurements

    CERN Document Server

    Iorio, Alberto Orso Maria

    2016-01-01

    Measurements of top quarks from Run-I and Run-II of the LHC are presented. Results on dif- ferential and inclusive top quark production cross sections, measured by the ATLAS, CMS and LHCb experiments, and measurements of top quark properties and mass are reported.

  8. Measurement of Color Texture

    NARCIS (Netherlands)

    Hoang, M.A.; Geusebroek, J.M.; Chantler, M.

    2002-01-01

    In computer vision, measurement of image properties such as color or texture is essential. In this paper, we propose a solid framework for the local measurement of texture in color images. We give a physical basis for the integration of the well-known Gabor filters with the measurement of color. Our

  9. Measurement of Color Texture

    NARCIS (Netherlands)

    Hoang, M.A.; Geusebroek, J.M.; Deprettere, E.F.; Belloum, A.; Heijnsdijk, J.W.J.; van der Stappen, F.

    2002-01-01

    In computer vision, measurement of image properties such as color or texture is essential. However, existing methods for measuring color and texture in combination are not well-defined neither from a measurement theoretical basis nor from a physical point of view. We propose a solid framework for th

  10. Coordinate measuring machines

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    This document is used in connection with three exercises of 2 hours duration as a part of the course GEOMETRICAL METROLOGY AND MACHINE TESTING. The exercises concern three aspects of coordinate measuring: 1) Measuring and verification of tolerances on coordinate measuring machines, 2) Traceability...

  11. Measurement System & Calibration report

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    This Measurement System & Calibration report is describing DTU’s measurement system installed at a specific wind turbine. A major part of the sensors has been installed by others (see [1]) the rest of the sensors have been installed by DTU. The results of the measurements, described in this report...

  12. Measurement Practice Guide

    Science.gov (United States)

    College and Career Readiness and Success Center, 2014

    2014-01-01

    This discussion guide is part of a larger practice guide designed to help state education agencies (SEAs) define measurement goals, select college and career readiness measures and indicators designed to support those goals, and use the data gathered with those measures and indicators to make informed decisions about college and career readiness…

  13. Teaching Measurement with Literature

    Science.gov (United States)

    Bintz, William P.; Moore, Sara D.

    2011-01-01

    Measurement is a difficult concept for many children. Trend data from the National Association of Educational Progress (NAEP) indicate that student achievement with measurement is disappointing, given the amount of instructional time it receives in K-grade 5. In an attempt to address the problem of student achievement with measurement, the authors…

  14. Measurement and Research Tools.

    Science.gov (United States)

    1997

    This document contains four papers from a symposium on measurement and research tools for human resource development (HRD). "The 'Best Fit' Training: Measure Employee Learning Style Strengths" (Daniel L. Parry) discusses a study of the physiological aspect of sensory intake known as modality, more specifically, modality as measured by…

  15. Power Curve Measurements

    DEFF Research Database (Denmark)

    Vesth, Allan; Kock, Carsten Weber

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present anal...

  16. Power Curve Measurements

    DEFF Research Database (Denmark)

    Villanueva, Héctor; Gómez Arranz, Paula

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...

  17. Power Curve Measurements FGW

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Villanueva, Héctor

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...

  18. Power Curve Measurements

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Vesth, Allan

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...

  19. Power Curve Measurements, FGW

    DEFF Research Database (Denmark)

    Vesth, Allan; Yordanova, Ginka

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...

  20. Power Curve Measurements, REWS

    DEFF Research Database (Denmark)

    Villanueva, Héctor; Gómez Arranz, Paula

    This report describes the power curve measurements carried out on a given wind turbine in a chosen period. The measurements were carried out following the measurement procedure in the draft of IEC 61400-12-1 Ed.2 [1], with some deviations mostly regarding uncertainty calculation. Here, the refere...

  1. Power Curve Measurements

    DEFF Research Database (Denmark)

    Federici, Paolo; Kock, Carsten Weber

    This report describes the power curve measurements performed with a nacelle LIDAR on a given wind turbine in a wind farm and during a chosen measurement period. The measurements and analysis are carried out in accordance to the guidelines in the procedure “DTU Wind Energy-E-0019” [1]. The reporting...

  2. The Measurement of Meaning.

    Science.gov (United States)

    Osgood, Charles E.; And Others

    The problem of measuring meaning viewed strictly as a psychological conception via the application of semantic differential theory is presented in this study. The first half of the book discusses theoretical background, logic of semantic measurement, basic factor analysis research, and a critical scientific evaluation of measuring procedures. The…

  3. Power Curve Measurements

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Federici, Paolo

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...... analyze of power performance of the turbine....

  4. Power Curve Measurements, FGW

    DEFF Research Database (Denmark)

    Kock, Carsten Weber; Vesth, Allan

    The report describes power curve measurements carried out on a given wind turbine. The measurements are carried out in accordance to Ref. [1]. A site calibration has been carried out; see Ref. [2], and the measured flow correction factors for different wind directions are used in the present...... analyze of power performance of the turbine....

  5. Measure Free Martingales and Martingale Measures

    Indian Academy of Sciences (India)

    M G Nadkarni; B Rajeev

    2009-11-01

    Let $T\\subset\\mathbb{R}$ be a countable set, not necessarily discrete. Let $f_t,t\\in T$, be a family of real-valued functions defined on a set . We discuss conditions which imply that there is a probability measure on under which the family $f_t,t\\in T$, is a martingale.

  6. Measurement of ambiguity tolerance.

    Science.gov (United States)

    Norton, R W

    1975-12-01

    Presented definitions for the construct of ambiguity tolerance. The measure of ambiguity tolerance (MAT-50) had high internal reliability (r = .88) and high test-retest reliability (r = .86) over a 10-to-12 week period. A content analysis of the measure and a subjective analysis by 20 graduate students indicated adequate content validity. A multivariate comparison with two other ambiguity measures, two rigidity measures, and a short dogmatism measure provided strong evidence for criteria-related validity. Finally, four independent empirical studies showed good construct validity.

  7. Current measurement apparatus

    Science.gov (United States)

    Umans, Stephen D.

    2008-11-11

    Apparatus and methods are provided for a system for measurement of a current in a conductor such that the conductor current may be momentarily directed to a current measurement element in order to maintain proper current without significantly increasing an amount of power dissipation attributable to the current measurement element or adding resistance to assist in current measurement. The apparatus and methods described herein are useful in superconducting circuits where it is necessary to monitor current carried by the superconducting elements while minimizing the effects of power dissipation attributable to the current measurement element.

  8. Stability of boundary measures

    CERN Document Server

    Chazal, Frédéric; Mérigot, Quentin

    2007-01-01

    We introduce the boundary measure at scale r of a compact subset of the n-dimensional Euclidean space. We show how it can be computed for point clouds and suggest these measures can be used for feature detection. The main contribution of this work is the proof a quantitative stability theorem for boundary measures using tools of convex analysis and geometric measure theory. As a corollary we obtain a stability result for Federer's curvature measures of a compact, allowing to compute them from point-cloud approximations of the compact.

  9. Consistent quantum measurements

    Science.gov (United States)

    Griffiths, Robert B.

    2015-11-01

    In response to recent criticisms by Okon and Sudarsky, various aspects of the consistent histories (CH) resolution of the quantum measurement problem(s) are discussed using a simple Stern-Gerlach device, and compared with the alternative approaches to the measurement problem provided by spontaneous localization (GRW), Bohmian mechanics, many worlds, and standard (textbook) quantum mechanics. Among these CH is unique in solving the second measurement problem: inferring from the measurement outcome a property of the measured system at a time before the measurement took place, as is done routinely by experimental physicists. The main respect in which CH differs from other quantum interpretations is in allowing multiple stochastic descriptions of a given measurement situation, from which one (or more) can be selected on the basis of its utility. This requires abandoning a principle (termed unicity), central to classical physics, that at any instant of time there is only a single correct description of the world.

  10. Traceability and Measurement Uncertainty

    DEFF Research Database (Denmark)

    Tosello, Guido; De Chiffre, Leonardo

    2004-01-01

    respects necessary scientific precision and problem-solving approach of the field of engineering studies. Competences should be presented in a way that is methodologically and didactically optimised for employees with a mostly work-based vocational qualification and should at the same time be appealing...... and motivating to this important group. The developed e-learning system consists on 12 different chapters dealing with the following topics: 1. Basics 2. Traceability and measurement uncertainty 3. Coordinate metrology 4. Form measurement 5. Surface testing 6. Optical measurement and testing 7. Measuring rooms 8....... Machine tool testing 9. The role of manufacturing metrology for QM 10. Inspection planning 11. Quality management of measurements incl. Documentation 12. Advanced manufacturing measurement technology The present report (which represents the section 2 - Traceability and Measurement Uncertainty – of the e...

  11. Measuring social value orientation

    Directory of Open Access Journals (Sweden)

    Ryan O. Murphy

    2011-12-01

    Full Text Available Narrow self-interest is often used as a simplifying assumption when studying people making decisions in social contexts. Nonetheless, people exhibit a wide range of different motivations when choosing unilaterally among interdependent outcomes. Measuring the magnitude of the concern people have for others, sometimes called Social Value Orientation (SVO, has been an interest of many social scientists for decades and several different measurement methods have been developed so far. Here we introduce a new measure of SVO that has several advantages over existent methods. A detailed description of the new measurement method is presented, along with norming data that provide evidence of its solid psychometric properties. We conclude with a brief discussion of the research streams that would benefit from a more sensitive and higher resolution measure of SVO, and extend an invitation to others to use this new measure which is freely available.

  12. Comparing Measures of Sparsity

    CERN Document Server

    Hurley, Niall P

    2008-01-01

    Sparsity of representations of signals has been shown to be a key concept of fundamental importance in fields such as blind source separation, compression, sampling and signal analysis. The aim of this paper is to compare several commonlyused sparsity measures based on intuitive attributes. Intuitively, a sparse representation is one in which a small number of coefficients contain a large proportion of the energy. In this paper six properties are discussed: (Robin Hood, Scaling, Rising Tide, Cloning, Bill Gates and Babies), each of which a sparsity measure should have. The main contributions of this paper are the proofs and the associated summary table which classify commonly-used sparsity measures based on whether or not they satisfy these six propositions and the corresponding proofs. Only one of these measures satisfies all six: The Gini Index. measures based on whether or not they satisfy these six propositions and the corresponding proofs. Only one of these measures satisfies all six: The Gini Index.

  13. Locke on measurement.

    Science.gov (United States)

    Anstey, Peter R

    2016-12-01

    Like many virtuosi in his day, the English philosopher John Locke maintained an active interest in metrology. Yet for Locke, this was no mere hobby: questions concerning measurement were also implicated in his ongoing philosophical project to develop an account of human understanding. This paper follows Locke's treatment of four problems of measurement from the early Drafts A and B of the Essay concerning Human Understanding to the publication of this famous book and its aftermath. It traces Locke's attempt to develop a natural or universal standard for the measure of length, his attempts to grapple with the measurement of duration, as well as the problems of determining comparative measures for secondary qualities, and the problem of discriminating small differences in the conventional measures of his day. It is argued that the salient context for Locke's treatment of these problems is the new experimental philosophy and its method of experimental natural history.

  14. Optimized measurement of gaps

    Science.gov (United States)

    Harding, Kevin; Ramamurthy, Rajesh

    2017-05-01

    Gaps are important in a wide range of measurements in manufacturing, from the fitting of critical assemblies too cosmetic features on cars. There are a variety of potential sensors that can measure a gap opening, each with aspects of gap measurements that they do well and other aspects where the technology may lack capability. This paper provides a review of a wide range of optical gages from structured light to passive systems and from line to area measurement. Each technology is considered relative to the ability to accurately measure a gap, including issues of edge effects, edge shape, surface finish, and transparency. Finally, an approach will be presented for creating an optimize measurement off gap openings for critical assembly applications.

  15. Regularities of Multifractal Measures

    Indian Academy of Sciences (India)

    Hun Ki Baek

    2008-05-01

    First, we prove the decomposition theorem for the regularities of multifractal Hausdorff measure and packing measure in $\\mathbb{R}^d$. This decomposition theorem enables us to split a set into regular and irregular parts, so that we can analyze each separately, and recombine them without affecting density properties. Next, we give some properties related to multifractal Hausdorff and packing densities. Finally, we extend the density theorem in [6] to any measurable set.

  16. Relativistic Quantum Noninvasive Measurements

    CERN Document Server

    Bednorz, Adam

    2014-01-01

    Quantum weak, noninvasive measurements are defined in the framework of relativity. Invariance with respect to reference frame transformations of the results in different models is discussed. Surprisingly, the bare results of noninvasive measurements are invariant for certain class of models, but not the detection error. Consequently, any stationary quantum realism based on noninvasive measurements will break, at least spontaneously, relativistic invariance and correspondence principle at zero temperature.

  17. Semiconductor Laser Measurements Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Semiconductor Laser Measurements Laboratory is equipped to investigate and characterize the lasing properties of semiconductor diode lasers. Lasing features such...

  18. Aperture area measurement facility

    Data.gov (United States)

    Federal Laboratory Consortium — NIST has established an absolute aperture area measurement facility for circular and near-circular apertures use in radiometric instruments. The facility consists of...

  19. Precision Electroweak Measurements

    CERN Document Server

    Przysiezniak, H

    2000-01-01

    This talk describes some of the precision electroweak measurements from around the world, namely those related to the Z and W bosons, the top quark mass, sin2 theta_ W at NuTeV, and three other fundamental measurements: alpha-1 m2_ Z, (g-2)_mu at the E821 BNL experiment as well as the atomic parity violation (APV) measurement for the Cesium atom. These and other measurements are set in the context of the Standard Model (SM) and of the electroweak fit predictions. Future prospects for forthcoming experiments are briefly discussed.

  20. Gamma measurements at LHCb

    CERN Document Server

    Cheung, Faye

    2016-01-01

    Recent measurements of the CKM angle g from charged and neutral B$\\rightarrow$ DK(*) decays, with a variety of D final states, are presented. The measurements are performed using proton-proton collision data corresponding to an integrated luminosity of 3 fb$^{-1}$ collected at the LHCb experiment during Run I of the LHC. The results of a combination of LHCb g measurements are discussed. The combined value of $\\gamma$ is $(70.9^{+7.1}_{-8.5})^{\\circ}$ and is the most precise measurement of $\\gamma$ from a single experiment.

  1. Coherent states measurement entropy

    CERN Document Server

    Kwapien, J; Zyczkowski, K; Kwapien, Jaroslaw; Slomczynski, Wojciech; Zyczkowski, Karol

    1996-01-01

    Coherent states (CS) quantum entropy can be split into two components. The dynamical entropy is linked with the dynamical properties of a quantum system. The measurement entropy, which tends to zero in the semiclassical limit, describes the unpredictability induced by the process of a quantum approximate measurement. We study the CS--measurement entropy for spin coherent states defined on the sphere discussing different methods dealing with the time limit n \\to \\infty. In particular we propose an effective technique of computing the entropy by iterated function systems. The dependence of CS--measurement entropy on the character of the partition of the phase space is analysed.

  2. Measuring Physical Activity Intensity

    Medline Plus

    Full Text Available ... Adults Overcoming Barriers Measuring Physical Activity Intensity Target Heart Rate & Estimated Maximum Heart Rate Perceived Exertion (Borg Rating of Perceived Exertion Scale) ...

  3. Photothermal measurements of superconductors

    Energy Technology Data Exchange (ETDEWEB)

    Kino, G.S.; Studenmund, W.R.; Fishman, I.M. [Stanford Univ., Stanford, CA (United States)

    1996-12-31

    A photothermal technique has been used to measure diffusion and critical temperature in high temperature superconductors. The technique is particularly suitable for determining material quality and inhomogeneity.

  4. Systemic risk measures

    Science.gov (United States)

    Guerra, Solange Maria; Silva, Thiago Christiano; Tabak, Benjamin Miranda; de Souza Penaloza, Rodrigo Andrés; de Castro Miranda, Rodrigo César

    2016-01-01

    In this paper we present systemic risk measures based on contingent claims approach and banking sector multivariate density. We also apply network measures to analyze bank common risk exposure. The proposed measures aim to capture credit risk stress and its potential to become systemic. These indicators capture not only individual bank vulnerability, but also the stress dependency structure between them. Furthermore, these measures can be quite useful for identifying systemically important banks. The empirical results show that these indicators capture with considerable fidelity the moments of increasing systemic risk in the Brazilian banking sector in recent years.

  5. Atmospheric Measurements Laboratory (AML)

    Data.gov (United States)

    Federal Laboratory Consortium — The Atmospheric Measurements Laboratory (AML) is one of the nation's leading research facilities for understanding aerosols, clouds, and their interactions. The AML...

  6. Measuring Mobile Phone Use

    DEFF Research Database (Denmark)

    Boase, Jeff; Ling, Richard

    2013-01-01

    In this paper we examine how well two types of self-report measures adequately operationalize frequency of mobile phone use by comparing them to server log data. Our self-report measures of voice and SMS text messaging activity are drawn from a nationally representative survey of adults living in...

  7. Dynamics via measurability

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available Generators f for σ -algebras can be used to view the dynamics of an invertible measurable transformation T in terms of the range values of f ∘ T . Such generators are the norm rather than the exception. Related measurable and quantitative methods of estimating a function from the behavior of ergodic averages are also discussed.

  8. Higgs Measurement from ATLAS

    CERN Document Server

    Liu, Bo; The ATLAS collaboration

    2015-01-01

    Using run-I data record by ATLAS detector, many searches on the higgs decay modes have been done. Also some properties measurement of the Higgs Boson has been done. This talk will summarise the recent result of Higgs search and measurement from ATLAS.

  9. Three measures of longevity

    DEFF Research Database (Denmark)

    Canudas-Romo, Vladimir

    2010-01-01

    This article examines the trend over time in the measures of "typical" longevity experienced by members of a population: life expectancy at birth, and the median and modal ages at death. The article also analyzes trends in record values observed for all three measures. The record life expectancy ...

  10. Measures of Biochemical Sociology

    Science.gov (United States)

    Snell, Joel; Marsh, Mitchell

    2008-01-01

    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  11. Measures of Pleasures

    NARCIS (Netherlands)

    S.J. Magala (Slawomir)

    2002-01-01

    textabstractMeasuring culture originated in cultural anthropology, but all social sciences contributed to comparative cultural studies. Tracing critical approaches towards a measurement of cultural values one is bound to strip the biases and stereotypes bare and to invade numerous academic fiefs. Ho

  12. Measuring News Media Literacy

    Science.gov (United States)

    Maksl, Adam; Ashley, Seth; Craft, Stephanie

    2015-01-01

    News media literacy refers to the knowledge and motivations needed to identify and engage with journalism. This study measured levels of news media literacy among 500 teenagers using a new scale measure based on Potter's model of media literacy and adapted to news media specifically. The adapted model posits that news media literate individuals…

  13. Measuring innovative work behaviour

    NARCIS (Netherlands)

    de Jong, J.; den Hartog, D.

    2010-01-01

    Both scientists and practitioners emphasize the importance of innovative work behaviour (IWB) of individual employees for organizational success, but the measurement of IWB is still at an evolutionary stage. This article is concerned with developed a measure of IWB with four potential dimensions: th

  14. Isothermal and Adiabatic Measurements.

    Science.gov (United States)

    McNairy, William W.

    1996-01-01

    Describes the working of the Adiabatic Gas Law Apparatus, a useful tool for measuring the pressure, temperature, and volume of a variety of gases undergoing compressions and expansions. Describes the adaptation of this apparatus to perform isothermal measurements and discusses the theory behind the adiabatic and isothermal processes. (JRH)

  15. Modeling typical performance measures

    NARCIS (Netherlands)

    Weekers, Anke Martine

    2009-01-01

    In the educational, employment, and clinical context, attitude and personality inventories are used to measure typical performance traits. Statistical models are applied to obtain latent trait estimates. Often the same statistical models as the models used in maximum performance measurement are appl

  16. Measuring Relational Reasoning

    Science.gov (United States)

    Alexander, Patricia A.; Dumas, Denis; Grossnickle, Emily M.; List, Alexandra; Firetto, Carla M.

    2016-01-01

    Relational reasoning is the foundational cognitive ability to discern meaningful patterns within an informational stream, but its reliable and valid measurement remains problematic. In this investigation, the measurement of relational reasoning unfolded in three stages. Stage 1 entailed the establishment of a research-based conceptualization of…

  17. Measuring Social Capital

    DEFF Research Database (Denmark)

    Svendsen, Gert Tinggaard; Bjørnskov, Christian

    2007-01-01

    How to construct a robust measure of social capital? This paper contains two contributions. The first is an attempt to establish a broad social capital measure based on four indicators, the Freedom House Index, an index of perceived corruption from Transparency International, and scores on civic...

  18. Faraday rotation measure synthesis

    NARCIS (Netherlands)

    Brentjens, MA; de Bruyn, AG

    2005-01-01

    We extend the rotation measure work of Burn ( 1966, MNRAS, 133, 67) to the cases of limited sampling of lambda(2) space and non-constant emission spectra. We introduce the rotation measure transfer function (RMTF), which is an excellent predictor of n pi ambiguity problems with the lambda(2) coverag

  19. Global Precipitation Measurement Poster

    Science.gov (United States)

    Azarbarzin, Art

    2010-01-01

    This poster presents an overview of the Global Precipitation Measurement (GPM) constellation of satellites which are designed to measure the Earth's precipitation. It includes the schedule of launches for the various satellites in the constellation, and the coverage of the constellation, It also reviews the mission capabilities, and the mission science objectives.

  20. Hand measuring eqipment

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    This note is used in connection with a 3 x 2 hours laboratory exercise as a part of the course GEOMETRICAL METROLOGY AND MACHINE TESTING. The laboratory includes a demonstration of a series of hand measuring tools as well as a number of exercises, illustrating the use of hand measuring equipment...

  1. Mechanical Measurements Laboratory

    CERN Multimedia

    Maximilien Brice

    2007-01-01

    The CERN mechanical measurements team check the sensors on one of the ATLAS inner detector end-caps using high precision measurement equipment. Remote checks like this must be made on these sensitive detector components before they can be transported to make sure that all systems are working correctly.

  2. Measuring innovative work behaviour

    NARCIS (Netherlands)

    de Jong, J.; den Hartog, D.

    2010-01-01

    Both scientists and practitioners emphasize the importance of innovative work behaviour (IWB) of individual employees for organizational success, but the measurement of IWB is still at an evolutionary stage. This article is concerned with developed a measure of IWB with four potential dimensions:

  3. Measurement of surface roughness

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    This document is used in connection with two 3 hours laboratory exercises that are part of the course GEOMETRICAL METROLOGY AND MACHINE TESTING. The laboratories include a demonstration of the function of roughness measuring instruments plus a series of exercises illustrating roughness measurement...

  4. Measuring internet skills

    NARCIS (Netherlands)

    Deursen, van A.J.A.M.; Dijk, van J.A.G.M.

    2010-01-01

    Research that considers Internet skills often lacks theoretical justifications and does not go beyond basic button knowledge. There is a strong need for a measurement framework that can guide future research. In this article, operational definitions for measuring Internet skills are proposed, applie

  5. Measuring Facial Movement

    Science.gov (United States)

    Ekman, Paul; Friesen, Wallace V.

    1976-01-01

    The Facial Action Code (FAC) was derived from an analysis of the anatomical basis of facial movement. The development of the method is explained, contrasting it to other methods of measuring facial behavior. An example of how facial behavior is measured is provided, and ideas about research applications are discussed. (Author)

  6. Measures of Biochemical Sociology

    Science.gov (United States)

    Snell, Joel; Marsh, Mitchell

    2008-01-01

    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  7. Metrology Measurement Capabilities

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Glen E. Gronniger

    2007-10-02

    This document contains descriptions of Federal Manufacturing & Technologies (FM&T) Metrology capabilities, traceability flow charts, and the measurement uncertainty of each measurement capability. Metrology provides NIST traceable precision measurements or equipment calibration for a wide variety of parameters, ranges, and state-of-the-art uncertainties. Metrology laboratories conform to the requirements of the Department of Energy Development and Production Manual Chapter 13.2, ANSI/ISO/IEC ANSI/ISO/IEC 17025:2005, and ANSI/NCSL Z540-1. FM&T Metrology laboratories are accredited by NVLAP for the parameters, ranges, and uncertainties listed in the specific scope of accreditation under NVLAP Lab code 200108-0. See the Internet at http://ts.nist.gov/Standards/scopes/2001080.pdf. These parameters are summarized. The Honeywell Federal Manufacturing & Technologies (FM&T) Metrology Department has developed measurement technology and calibration capability in four major fields of measurement: (1) Mechanical; (2) Environmental, Gas, Liquid; (3) Electrical (DC, AC, RF/Microwave); and (4) Optical and Radiation. Metrology Engineering provides the expertise to develop measurement capabilities for virtually any type of measurement in the fields listed above. A strong audit function has been developed to provide a means to evaluate the calibration programs of our suppliers and internal calibration organizations. Evaluation includes measurement audits and technical surveys.

  8. Measuring Poverty: A Rejoinder

    Science.gov (United States)

    Iceland, John

    2005-01-01

    This article presents the author's rejoinder on commentaries of his article which illustrate the variety of perspectives with which people approach poverty measurement issues. Some of the comments highlight the theoretical concerns underpinning poverty measurement efforts, whereas others focus on empirical considerations. As a social scientist,…

  9. Measure Free Martingales

    Indian Academy of Sciences (India)

    Rajeeva L Karandikar; M G Nadkarni

    2005-02-01

    We give a necessary and sufficient condition on a sequence of functions on a set under which there is a measure on which renders the given sequence of functions a martingale. Further such a measure is unique if we impose a natural maximum entropy condition on the conditional probabilities.

  10. Electron measurement in PHENIX

    Energy Technology Data Exchange (ETDEWEB)

    Akiba, Y. [Univ. of Tokyo, Tanashi (Japan)

    1995-07-15

    Electron Measurement in PHENIX detector at RHIC is discussed. The yield and S/N ratio at vector meson peaks ({phi}, {omega}, {rho}{sup o}, and J/{psi}) are evaluated. The electrons from open charm decay, and its consequence to the di-electron measurements is discussed.

  11. Refractive index based measurements

    DEFF Research Database (Denmark)

    2014-01-01

    A refractive index based measurement of a property of a fluid is measured in an apparatus comprising a variable wavelength coherent light source (16), a sample chamber (12), a wavelength controller (24), a light sensor (20), a data recorder (26) and a computation apparatus (28), by - directing...

  12. Refractive index based measurements

    DEFF Research Database (Denmark)

    2014-01-01

    In a method for performing a refractive index based measurement of a property of a fluid such as chemical composition or temperature by observing an apparent angular shift in an interference fringe pattern produced by back or forward scattering interferometry, ambiguities in the measurement caused...

  13. Bullen Reading Attitude Measure.

    Science.gov (United States)

    Bullen, Gertrude F.

    The Bullen Reading Attitude Measure (BRAM) is an instrument that was developed to serve as a diagnostic aid in assessing reading attitudes of elementary school children in grades one through six. The objectives of the test are to measure the subject's attitude toward reading at home or school, visiting the library, owning and buying books,…

  14. Aerial measurements in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Winkelmann, I.; Thomas, M.; Buchroeder, H.; Brummer, C. [Federal Office for Radiation Protection, Berlin (Germany); Carloff, G. [German Federal Border Police, Grenzschutz-Fliegergruppe, Sankt Augustin (Germany)

    1997-12-31

    Aerial measurements were performed to determine the {sup 137}Cs soil contamination in a given region to detect unknown radiation sources and to assess their activity. For these measurements a computerized gamma ray spectrometer, equipped with a high purity Ge-semiconductor detector and a 12 l volume Nal(Tl)-detector was used. HPGe-detector measurements from different altitudes over area I were done to test and re-calibrate the aerial measuring system. The known {sup 137}Cs contamination of (50.7 {+-} 5.2) kBq m{sup -2} could be confirmed by the measured value of (57 {+-} 10) kBq m{sup -2}. the Nal(Tl)-detector was re-calibrated at that site for further {sup 137}Cs measurements over area II. The area II was surveyed from an altitude of about 70 m and at a parallel line distance of 150 m at an flying speed of 100 km h{sup -1} to determine the {sup 137}Cs soil contamination. The measuring time was two seconds for the Nal(Tl)-detector. For the spectra measured with the HPGe-detector, a measuring time of 30 s each was chosen. From the Nal(Tl)-measurements, a mean {sup 137}Cs value of (60 {+-} 20) kBq m{sup -2} was determined with a maximum value of 90 kBq m{sup -2}. The corresponding values measured by HPGe-detector were (70 {+-} 20) kBq m{sup -2} and 120 kBq m{sup -2}, respectively. For the evaluation of the HPGe-spectra a depth distribution parameter {alpha}/{rho} = (0.44 {+-} 0.21) cm{sup 2} g{sup -1} for {sup 137}Cs was used measured from soil samples. From data measured with the Nal(Tl)-detector during flights over area III, three{sup 60}Co-sources and one {sup 137}Cs source could be detected, localized and their activity assessed. By HPGe-detector measurements, only scattered {sup 192}lr radiation was registered. (au).

  15. W mass measurement

    CERN Document Server

    Andari, Nansi; The ATLAS collaboration

    2017-01-01

    A first measurement of the W-boson mass at the LHC is presented based on about 4.6 fb^-1 of proton–proton collision data recorded in 2011 at a centre-of-mass energy of 7 TeV with the ATLAS detector. The measured value is 80370±19 MeV, consistent with the Standard Model prediction, with the combined values measured at the LEP and Tevatron colliders, and with the world average. The ATLAS result equals in precision the previous best measurement of the W mass, performed by the CDF collaboration. The CMS results for the W-like measurement of the Z boson mass using dimuon events are also shown. An overview of the analyses is shown with a special emphasis on the evaluation of the experimental systematic uncertainties, as well as on the uncertainties due to the modelling of the vector boson production and decay.

  16. Measuring static complexity

    Directory of Open Access Journals (Sweden)

    Ben Goertzel

    1992-01-01

    Full Text Available The concept of “pattern” is introduced, formally defined, and used to analyze various measures of the complexity of finite binary sequences and other objects. The standard Kolmogoroff-Chaitin-Solomonoff complexity measure is considered, along with Bennett's ‘logical depth’, Koppel's ‘sophistication'’, and Chaitin's analysis of the complexity of geometric objects. The pattern-theoretic point of view illuminates the shortcomings of these measures and leads to specific improvements, it gives rise to two novel mathematical concepts--“orders” of complexity and “levels” of pattern, and it yields a new measure of complexity, the “structural complexity”, which measures the total amount of structure an entity possesses.

  17. Undoing a quantum measurement.

    Science.gov (United States)

    Schindler, Philipp; Monz, Thomas; Nigg, Daniel; Barreiro, Julio T; Martinez, Esteban A; Brandl, Matthias F; Chwalla, Michael; Hennrich, Markus; Blatt, Rainer

    2013-02-15

    In general, a quantum measurement yields an undetermined answer and alters the system to be consistent with the measurement result. This process maps multiple initial states into a single state and thus cannot be reversed. This has important implications in quantum information processing, where errors can be interpreted as measurements. Therefore, it seems that it is impossible to correct errors in a quantum information processor, but protocols exist that are capable of eliminating them if they affect only part of the system. In this work we present the deterministic reversal of a fully projective measurement on a single particle, enabled by a quantum error-correction protocol in a trapped ion quantum information processor. We further introduce an in-sequence, single-species recooling procedure to counteract the motional heating of the ion string due to the measurement.

  18. Measurement of academic entitlement.

    Science.gov (United States)

    Miller, Brian K

    2013-10-01

    Members of Generation Y, or Millennials, have been accused of being lazy, whiny, pampered, and entitled, particularly in the college classroom. Using an equity theory framework, eight items from a measure of work entitlement were adapted to measure academic entitlement in a university setting in three independent samples. In Study 1 (n = 229), confirmatory factor analyses indicated good model fit to a unidimensional structure for the data. In Study 2 (n = 200), the questionnaire predicted unique variance in university satisfaction beyond two more general measures of dispositional entitlement. In Study 3 (n = 161), the measure predicted unique variance in perceptions of grade fairness beyond that which was predicted by another measure of academic entitlement. This analysis provides evidence of discriminant, convergent, incremental, concurrent criterion-related, and construct validity for the Academic Equity Preference Questionnaire.

  19. Stability of Curvature Measures

    CERN Document Server

    Chazal, Frédéric; Lieutier, André; Thibert, Boris

    2008-01-01

    We address the problem of curvature estimation from sampled compact sets. The main contribution is a stability result: we show that the gaussian, mean or anisotropic curvature measures of the offset of a compact set K with positive $\\mu$-reach can be estimated by the same curvature measures of the offset of a compact set K' close to K in the Hausdorff sense. We show how these curvature measures can be computed for finite unions of balls. The curvature measures of the offset of a compact set with positive $\\mu$-reach can thus be approximated by the curvature measures of the offset of a point-cloud sample. These results can also be interpreted as a framework for an effective and robust notion of curvature.

  20. Measurement uncertainty and probability

    CERN Document Server

    Willink, Robin

    2013-01-01

    A measurement result is incomplete without a statement of its 'uncertainty' or 'margin of error'. But what does this statement actually tell us? By examining the practical meaning of probability, this book discusses what is meant by a '95 percent interval of measurement uncertainty', and how such an interval can be calculated. The book argues that the concept of an unknown 'target value' is essential if probability is to be used as a tool for evaluating measurement uncertainty. It uses statistical concepts, such as a conditional confidence interval, to present 'extended' classical methods for evaluating measurement uncertainty. The use of the Monte Carlo principle for the simulation of experiments is described. Useful for researchers and graduate students, the book also discusses other philosophies relating to the evaluation of measurement uncertainty. It employs clear notation and language to avoid the confusion that exists in this controversial field of science.