Status of NIF mirror technologies for completion of the NIF facility

International Nuclear Information System (INIS)

Stolz, C.J.

2008-01-01

The 1600 mirrors required for the National Ignition Facility (NIF) are now coated with the last optics currently being installed. The combined surface area of the NIF mirrors is almost 450 square meters, roughly 3.4 times greater than the surface area of the two Keck primary mirrors. Additionally, the power handling specification of NIF mirrors is 19 orders of magnitude greater than that of the Keck mirrors. The NIF laser will be at least 40x greater energy than the previous LLNL fusion laser called NOVA. To manufacture these mirrors, a number of new technologies (electrolytic in-situ dressing, ion figuring, source stabilization) were used that were not available for previous fusion laser optics. Post deposition technologies designed to increase laser resistance (off-line laser conditioning, solarization, air knives) have also been utilized. This paper summarizes the differences in technologies used to manufacture NIF mirrors from those used for previous fusion lasers and examines potential future technologies that would enable higher fluence operations and extend lifetimes

Testing and Quality Assurance of the Control System During NIF Commissioning

International Nuclear Information System (INIS)

Casavant, D.; Carey, R.; Cline, B.; Lagin, L.; Ludwigsen, P.; Reddi, U.; Van Arsdall, P.

2003-01-01

The strategy used to develop the National Ignition Facility Integrated Computer Control System (NIF ICCS) calls for incremental cycles of construction and formal test to deliver nearly one million lines of code. Software releases that implement specific functionality are approved for deployment when offline tests conducted in the ICCS Integration and Test Facility verify functional, performance and interface requirements using test procedures derived from system requirements. At this stage of the project the controls team has delivered approximately 3/4 of the planned software by performing dozens of development and test cycles within offline test facilities and followed by online tests to confirm integrated operation in the NIF. Test incidents are recorded and tracked from development to successful deployment by the verification team, with hardware and software changes approved by the appropriate change control board. Project metrics are generated by the Software Quality Assurance manager and monitored by ICCS management. Test results are summarized and reported to responsible individuals and Project managers under a work authorization and permit process that assesses risk and evaluates control system upgrade readiness. NIF is well into the first phases of its laser commissioning program to characterize and operate the first four laser beams and target systems. The integrated control system has successfully fired over 100 coordinated shots into beam diagnostics and an initial set of target diagnostics in the 10-m diameter target chamber. Extensive experience has been gained by integrating controls in prototype laboratories and in the NIF. This paper will discuss NIF's software QC and QA processes, capabilities of offline test facilities, and metrics collection

The expression of nifB gene from Herbaspirillum seropedicae is dependent upon the NifA and RpoN proteins.

Science.gov (United States)

Rego, Fabiane G M; Pedrosa, Fábio O; Chubatsu, Leda S; Yates, M Geoffrey; Wassem, Roseli; Steffens, Maria B R; Rigo, Liu U; Souza, Emanuel M

2006-12-01

The putative nifB promoter region of Herbaspirillum seropedicae contained two sequences homologous to NifA-binding site and a -24/-12 type promoter. A nifB::lacZ fusion was assayed in the backgrounds of both Escherichia coli and H. seropedicae. In E. coli, the expression of nifB::lacZ occurred only in the presence of functional rpoN and Klebsiella pneumoniae nifA genes. In addition, the integration host factor (IHF) stimulated the expression of the nifB::lacZ fusion in this background. In H. seropedicae, nifB expression occurred only in the absence of ammonium and under low levels of oxygen, and it was shown to be strictly dependent on NifA. DNA band shift experiments showed that purified K. pneumoniae RpoN and E. coli IHF proteins were capable of binding to the nifB promoter region, and in vivo dimethylsulfate footprinting showed that NifA binds to both NifA-binding sites. These results strongly suggest that the expression of the nifB promoter of H. seropedicae is dependent on the NifA and RpoN proteins and that the IHF protein stimulates NifA activation of nifB promoter.

Mutant Forms of the Azotobacter vinelandii Transcriptional Activator NifA Resistant to Inhibition by the NifL Regulatory Protein

OpenAIRE

Reyes-Ramirez, Francisca; Little, Richard; Dixon, Ray

2002-01-01

The Azotobacter vinelandii σ54-dependent transcriptional activator protein NifA is regulated by the NifL protein in response to redox, carbon, and nitrogen status. Under conditions inappropriate for nitrogen fixation, NifL inhibits transcription activation by NifA through the formation of the NifL-NifA protein complex. NifL inhibits the ATPase activity of the central AAA+ domain of NifA required to drive open complex formation by σ54-RNA polymerase and may also inhibit the activator-polymeras...

VTVH-MCD study of the Delta nifB Delta nifZ MoFe protein from Azotobacter vinelandii.

Science.gov (United States)

Cotton, Marcia S; Rupnik, Kresimir; Broach, Robyn B; Hu, Yilin; Fay, Aaron W; Ribbe, Markus W; Hales, Brian J

2009-04-08

NifZ is a member of a series of proteins associated with the maturation of the nitrogenase MoFe protein. An MCD spectroscopic study was undertaken on the Delta nifB Delta nifZ MoFe protein generated in the absence of both NifZ and NifB (deletion of NifB generates an apo-MoFe protein lacking the FeMo cofactor). Results presented here show that, in the absence of NifZ, only one of the two P-clusters of the MoFe protein is matured to the ultimate [8Fe-7S] structure. The other P-cluster site in the protein contains a [4Fe-4S] cluster pair, representing a P-cluster precursor that is electronically identical to the analogous clusters observed in the Delta nifH MoFe protein. These results suggest that the MoFe protein is synthesized in a stepwise fashion where NifZ is specifically required for the formation of the second P-cluster.

Testing and Quality Assurance of the Control System During NIF Commissioning

Energy Technology Data Exchange (ETDEWEB)

Casavant, D; Carey, R; Cline, B; Lagin, L; Ludwigsen, P; Reddi, U; Van Arsdall, P

2003-10-13

The strategy used to develop the National Ignition Facility Integrated Computer Control System (NIF ICCS) calls for incremental cycles of construction and formal test to deliver nearly one million lines of code. Software releases that implement specific functionality are approved for deployment when offline tests conducted in the ICCS Integration and Test Facility verify functional, performance and interface requirements using test procedures derived from system requirements. At this stage of the project the controls team has delivered approximately 3/4 of the planned software by performing dozens of development and test cycles within offline test facilities and followed by online tests to confirm integrated operation in the NIF. Test incidents are recorded and tracked from development to successful deployment by the verification team, with hardware and software changes approved by the appropriate change control board. Project metrics are generated by the Software Quality Assurance manager and monitored by ICCS management. Test results are summarized and reported to responsible individuals and Project managers under a work authorization and permit process that assesses risk and evaluates control system upgrade readiness. NIF is well into the first phases of its laser commissioning program to characterize and operate the first four laser beams and target systems. The integrated control system has successfully fired over 100 coordinated shots into beam diagnostics and an initial set of target diagnostics in the 10-m diameter target chamber. Extensive experience has been gained by integrating controls in prototype laboratories and in the NIF. This paper will discuss NIF's software QC and QA processes, capabilities of offline test facilities, and metrics collection.

NIF Laser Line Replaceable Units (LRUs)

International Nuclear Information System (INIS)

Larson, D W

2003-01-01

The National Ignition Facility (NIF) is designed with its high value optical systems in cassettes called Line Replaceable Units (LRUs). Virtually all of the NIF's active components are assembled in one of the ∼4000 electrical and optical LRUs that serve between two and eight of NIF's 192 laser beam lines. Many of these LRUs are optomechanical assemblies that are roughly the size of a telephone booth. The primary design challenges for this hardware include meeting stringent mechanical precision, stability and cleanliness requirements. Pre-production units of each LRU type have been fielded on the first bundle of NIF and used to demonstrate that NIF meets its performance objectives. This presentation provides an overview of the NIF LRUs, their design and production plans for building out the remaining NIF bundles

Magnetic Fields on the National Ignition Facility (MagNIF)

International Nuclear Information System (INIS)

Mason, D.; Folta, J.

2016-01-01

A magnetized target capability on the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) has been investigated. Stakeholders' needs and project feasibility analysis were considered in order to down-select from a wide variety of different potential magnetic field magnitudes and volumes. From the large range of different target platforms, laser configurations, and diagnostics configurations of interest to the stakeholders, the gas-pipe platform has been selected for the first round of magnetized target experiments. Gas pipe targets are routinely shot on the NIF and provide unique value for external collaborators. High-level project goals have been established including an experimentally relevant 20Tesla magnetic field magnitude. The field will be achieved using pulsed power-driven coils. A system architecture has been proposed. The pulsed power drive system will be located in the NIF target bay. This decision provides improved maintainability and mitigates equipment safety risks associated with explosive failure of the drive capacitor. High-level and first-level subsystem requirements have been established. Requirements have been included for two distinct coil designs - full solenoid and quasi-Helmholtz. A Failure Modes and Effects Analysis (FMEA) has been performed and documented. Additional requirements have been derived from the mitigations included in the FMEA document. A project plan is proposed. The plan includes a first phase of electromagnetic simulations to assess whether the design will meet performance requirements, then a second phase of risk mitigation projects to address the areas of highest technical risk. The duration from project kickoff to the first magnetized target shot is approximately 29 months.

Magnetic Fields on the National Ignition Facility (MagNIF)

Energy Technology Data Exchange (ETDEWEB)

Mason, D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Folta, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2016-08-12

A magnetized target capability on the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) has been investigated. Stakeholders’ needs and project feasibility analysis were considered in order to down-select from a wide variety of different potential magnetic field magnitudes and volumes. From the large range of different target platforms, laser configurations, and diagnostics configurations of interest to the stakeholders, the gas-pipe platform has been selected for the first round of magnetized target experiments. Gas pipe targets are routinely shot on the NIF and provide unique value for external collaborators. High-level project goals have been established including an experimentally relevant 20Tesla magnetic field magnitude. The field will be achieved using pulsed power-driven coils. A system architecture has been proposed. The pulsed power drive system will be located in the NIF target bay. This decision provides improved maintainability and mitigates equipment safety risks associated with explosive failure of the drive capacitor. High-level and first-level subsystem requirements have been established. Requirements have been included for two distinct coil designs – full solenoid and quasi-Helmholtz. A Failure Modes and Effects Analysis (FMEA) has been performed and documented. Additional requirements have been derived from the mitigations included in the FMEA document. A project plan is proposed. The plan includes a first phase of electromagnetic simulations to assess whether the design will meet performance requirements, then a second phase of risk mitigation projects to address the areas of highest technical risk. The duration from project kickoff to the first magnetized target shot is approximately 29 months.

Applied superconductivity and cryogenic research activities in NIFS

International Nuclear Information System (INIS)

Mito, T.; Sagara, A.; Imagawa, S.; Yamada, S.; Takahata, K.; Yanagi, N.; Chikaraishi, H.; Maekawa, R.; Iwamoto, A.; Hamaguchi, S.; Sato, M.; Noda, N.; Yamauchi, K.; Komori, A.; Motojima, O.

2006-01-01

Since the foundation of National Institute for Fusion Science (NIFS) in 1989, the primary mission of the applied superconductivity and cryogenic researches has been focused on the development of the large helical device (LHD): the largest fusion experimental apparatus exclusively utilizing superconducting technologies. The applied superconductivity and cryogenics group in NIFS was organized to be responsible for this activity. As a result of extensive research activities, the construction of LHD was completed in 1997. Since then, the LHD superconducting system has been demonstrating high availability of more than 97% during eight years operation and it keeps proving high reliability of large-scale superconducting systems. This paper describes the extensive activities of the applied superconductivity and cryogenic researches in NIFS during and after the development of LHD and the fundamental researches that aim at realizing a helical-type fusion reactor

National NIF Diagnostic Program Fiscal Year 2002 Second Quarter Report

International Nuclear Information System (INIS)

MacGowan, B

2002-01-01

Since October 2001 the development of the facility diagnostics for NIF has been funded by the NIF Director through the National NIF Diagnostic Program (NNDP). The current emphasis of the NNDP is on diagnostics for the early NIF quad scheduled to be available for experiment commissioning in FY03. During the past six months the NNDP has set in place processes for funding diagnostics, developing requirements for diagnostics, design reviews and monthly status reporting. Those processes are described in an interim management plan for diagnostics (National NIF Diagnostic Program Interim Plan, NIF-008 13 15, April 2002) and a draft Program Execution Plan (Program Execution Plan for the National NlF Diagnostic Program, NIF-0072083, October 2001) and documents cited therein. Work has been funded at Lawrence Livermore National Laboratory (LLNL), Los Alamos National Laboratory (LANL), Naval Research Laboratory (NRL), Sandia National Laboratories (SNL), Bechtel Nevada at Los Alamos and Santa Barbara. There are no major technical risks with the early diagnostics. The main concerns relate to integration of the diagnostics into the facility, all such issues are being worked. This report is organized to show the schedule and budget status and a summary of Change Control Board actions for the past six months. The following sections then provide short descriptions of the status of each diagnostic. Where design reviews or requirements documents are cited, the documents are available on the Diagnostics file server or on request

Investigation into the MgF2-NiF2, CaF2-NiF2, SrF2-NiF2 systems

International Nuclear Information System (INIS)

Ikrami, D.D.; Petrov, S.V.; Fedorov, P.P.; Ol'khovaya, L.A.; Luginina, A.A.; AN SSSR, Moscow. Inst. Fizicheskikh Problem; AN SSSR, Moscow. Inst. Kristallografii)

1984-01-01

Using the methods of differential thermal and X-ray phase analyses the systems MgF 2 -NiF 2 , CaF 2 -NiF 2 , SrF 2 -NiF 2 have been studied. In the system SrF 2 -NiF 2 the only orthorhombic compounds SrNiF 4 (a=14.43; b=3.93; c=5.66 (+-0.01 A)) is formed. SrNiF 4 density constitutes: dsub(X-ray)=4.60+-0.01 g/cm 3 , dsub(exp.)=4.60+-0.03 g/cm 3 . Refraction indices are as follows SrNiF 4 :Ng=1.500; Nsub(m)=1.497; Nsub(p)=1.479. SrNiF 4 magnetic ordering temperature Tsub(N) approximately 100 K

The National Ignition Facility (NIF): A path to fusion energy

International Nuclear Information System (INIS)

Moses, Edward I.

2008-01-01

Fusion energy has long been considered a promising, clean, nearly inexhaustible source of energy. Power production by fusion micro-explosions of inertial confinement fusion (ICF) targets has been a long-term research goal since the invention of the first laser in 1960. The National Ignition Facility (NIF) is poised to take the next important step in the journey by beginning experiments researching ICF ignition. Ignition on NIF will be the culmination of over 30 years of ICF research on high-powered laser systems such as the Nova laser at Lawrence Livermore National Laboratory (LLNL) and the OMEGA laser at the University of Rochester, as well as smaller systems around the world. NIF is a 192-beam Nd-glass laser facility at LLNL that is more than 90% complete. The first cluster of 48 beams is operational in the laser bay, the second cluster is now being commissioned, and the beam path to the target chamber is being installed. The Project will be completed in 2009, and ignition experiments will start in 2010. When completed, NIF will produce up to 1.8 MJ of 0.35-μm light in highly shaped pulses required for ignition. It will have beam stability and control to higher precision than any other laser fusion facility. Experiments using one of the beams of NIF have demonstrated that NIF can meet its beam performance goals. The National Ignition Campaign (NIC) has been established to manage the ignition effort on NIF. NIC has all of the research and development required to execute the ignition plan and to develop NIF into a fully operational facility. NIF will explore the ignition space, including direct drive, 2ω ignition, and fast ignition, to optimize target efficiency for developing fusion as an energy source. In addition to efficient target performance, fusion energy requires significant advances in high-repetition-rate lasers and fusion reactor technology. The Mercury laser at LLNL is a high-repetition-rate Nd-glass laser for fusion energy driver development. Mercury

The national ignition facility (NIF) : A path to fusion energy

International Nuclear Information System (INIS)

Moses, E. I.

2007-01-01

Fusion energy has long been considered a promising clean, nearly inexhaustible source of energy. Power production by fusion micro-explosions of inertial confinement fusion (ICF) targets has been a long term research goal since the invention of the first laser in 1960. The NIF is poised to take the next important step in the journey by beginning experiments researching ICF ignition. Ignition on NIF will be the culmination of over thirty years of ICF research on high-powered laser systems such as the Nova laser at LLNL and the OMEGA laser at the University of Rochester as well as smaller systems around the world. NIF is a 192 beam Nd-glass laser facility at LLNL that is more than 90% complete. The first cluster of 48 beams is operational in the laser bay, the second cluster is now being commissioned, and the beam path to the target chamber is being installed. The Project will be completed in 2009 and ignition experiments will start in 2010. When completed NIF will produce up to 1.8 MJ of 0.35 μm light in highly shaped pulses required for ignition. It will have beam stability and control to higher precision than any other laser fusion facility. Experiments using one of the beams of NIF have demonstrated that NIF can meet its beam performance goals. The National Ignition Campaign (NIC) has been established to manage the ignition effort on NIF. NIC has all of the research and development required to execute the ignition plan and to develop NIF into a fully operational facility. NIF will explore the ignition space, including direct drive, 2ω ignition, and fast ignition, to optimize target efficiency for developing fusion as an energy source. In addition to efficient target performance, fusion energy requires significant advances in high repetition rate lasers and fusion reactor technology. The Mercury laser at LLNL is a high repetition rate Nd-glass laser for fusion energy driver development. Mercury uses state-o-the art technology such as ceramic laser slabs and light

National Ignition Facility Project Site Safety Program

International Nuclear Information System (INIS)

Dun, C

2003-01-01

This Safety Program for the National Ignition Facility (NIF) presents safety protocols and requirements that management and workers shall follow to assure a safe and healthful work environment during activities performed on the NIF Project site. The NIF Project Site Safety Program (NPSSP) requires that activities at the NIF Project site be performed in accordance with the ''LLNL ES and H Manual'' and the augmented set of controls and processes described in this NIF Project Site Safety Program. Specifically, this document: (1) Defines the fundamental NIF site safety philosophy. (2) Defines the areas covered by this safety program (see Appendix B). (3) Identifies management roles and responsibilities. (4) Defines core safety management processes. (5) Identifies NIF site-specific safety requirements. This NPSSP sets forth the responsibilities, requirements, rules, policies, and regulations for workers involved in work activities performed on the NIF Project site. Workers are required to implement measures to create a universal awareness that promotes safe practice at the work site and will achieve NIF management objectives in preventing accidents and illnesses. ES and H requirements are consistent with the ''LLNL ES and H Manual''. This NPSSP and implementing procedures (e.g., Management Walkabout, special work procedures, etc.,) are a comprehensive safety program that applies to NIF workers on the NIF Project site. The NIF Project site includes the B581/B681 site and support areas shown in Appendix B

Federated access to heterogeneous information resources in the Neuroscience Information Framework (NIF).

Science.gov (United States)

Gupta, Amarnath; Bug, William; Marenco, Luis; Qian, Xufei; Condit, Christopher; Rangarajan, Arun; Müller, Hans Michael; Miller, Perry L; Sanders, Brian; Grethe, Jeffrey S; Astakhov, Vadim; Shepherd, Gordon; Sternberg, Paul W; Martone, Maryann E

2008-09-01

The overarching goal of the NIF (Neuroscience Information Framework) project is to be a one-stop-shop for Neuroscience. This paper provides a technical overview of how the system is designed. The technical goal of the first version of the NIF system was to develop an information system that a neuroscientist can use to locate relevant information from a wide variety of information sources by simple keyword queries. Although the user would provide only keywords to retrieve information, the NIF system is designed to treat them as concepts whose meanings are interpreted by the system. Thus, a search for term should find a record containing synonyms of the term. The system is targeted to find information from web pages, publications, databases, web sites built upon databases, XML documents and any other modality in which such information may be published. We have designed a system to achieve this functionality. A central element in the system is an ontology called NIFSTD (for NIF Standard) constructed by amalgamating a number of known and newly developed ontologies. NIFSTD is used by our ontology management module, called OntoQuest to perform ontology-based search over data sources. The NIF architecture currently provides three different mechanisms for searching heterogeneous data sources including relational databases, web sites, XML documents and full text of publications. Version 1.0 of the NIF system is currently in beta test and may be accessed through http://nif.nih.gov.

Comparison of the Three NIF Ablators

Energy Technology Data Exchange (ETDEWEB)

Kritcher, A. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Clark, D. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Haan, S. W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Yi, S. A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Zylstra, A. B. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Ralph, J. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Weber, C. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-11-01

Indirect drive implosion experiments on NIF have now been performed using three different ablator materials: glow discharge polymer (GDP) or CH, high density carbon (HDC, which we also refer to as diamond), and sputtered beryllium (Be). It has been appreciated for some time that each of these materials has specific advantages and disadvantages as an ICF ablator.[1-4] In light of experiments conducted on NIF in the last few years, how do these ablators compare? Given current understanding, is any ablator more or less likely to reach ignition on NIF? Has the understanding of their respective strengths and weaknesses changed since NIF experiments began? How are those strengths and weaknesses highlighted by implosion designs currently being tested or planned for testing soon? This document aims to address these questions by combining modern simulation results with a survey of the current experimental data base. More particularly, this document is meant to fulfill an L2 Milestone for FY17 to “Document our understanding of the relative advantages and disadvantages of CH, HDC, and Be designs.” Note that this document does not aim to recommend a down-selection of the current three ablator choices. It is intended only to gather and document the current understanding of the differences between these ablators and thereby inform the choices made in planning future implosion experiments. This document has two themes: (i) We report on a reanalysis project in which post-shot simulations were done on a common basis for layered shots using each ablator. This included data from keyholes, 2D ConA, and so forth, from each campaign, leading up to the layered shots. (“Keyholes” are shots dedicated to measuring the shock timing in a NIF target, as described in Ref. 5. “2DConAs” are backlit implosions in which the symmetry of the implosion is measured between about half and full convergence, as described in Ref. 6.) This set of common-basis postshot simulations is compared to

A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria.

Science.gov (United States)

Gaby, John Christian; Buckley, Daniel H

2014-01-01

We describe a nitrogenase gene sequence database that facilitates analysis of the evolution and ecology of nitrogen-fixing organisms. The database contains 32 954 aligned nitrogenase nifH sequences linked to phylogenetic trees and associated sequence metadata. The database includes 185 linked multigene entries including full-length nifH, nifD, nifK and 16S ribosomal RNA (rRNA) gene sequences. Evolutionary analyses enabled by the multigene entries support an ancient horizontal transfer of nitrogenase genes between Archaea and Bacteria and provide evidence that nifH has a different history of horizontal gene transfer from the nifDK enzyme core. Further analyses show that lineages in nitrogenase cluster I and cluster III have different rates of substitution within nifD, suggesting that nifD is under different selection pressure in these two lineages. Finally, we find that that the genetic divergence of nifH and 16S rRNA genes does not correlate well at sequence dissimilarity values used commonly to define microbial species, as stains having <3% sequence dissimilarity in their 16S rRNA genes can have up to 23% dissimilarity in nifH. The nifH database has a number of uses including phylogenetic and evolutionary analyses, the design and assessment of primers/probes and the evaluation of nitrogenase sequence diversity. Database URL: http://www.css.cornell.edu/faculty/buckley/nifh.htm.

Enhanced oxygen consumption in Herbaspirillum seropedicae fnr mutants leads to increased NifA mediated transcriptional activation.

Science.gov (United States)

Batista, Marcelo Bueno; Wassem, Roseli; Pedrosa, Fábio de Oliveira; de Souza, Emanuel Maltempi; Dixon, Ray; Monteiro, Rose Adele

2015-05-07

Orthologous proteins of the Crp/Fnr family have been previously implicated in controlling expression and/or activity of the NifA transcriptional activator in some diazotrophs. This study aimed to address the role of three Fnr-like proteins from H. seropedicae SmR1 in controlling NifA activity and consequent NifA-mediated transcription activation. The activity of NifA-dependent transcriptional fusions (nifA::lacZ and nifB::lacZ) was analysed in a series of H. seropedicae fnr deletion mutant backgrounds. We found that combined deletions in both the fnr1 and fnr3 genes lead to higher expression of both the nifA and nifB genes and also an increased level of nifH transcripts. Expression profiles of nifB under different oxygen concentrations, together with oxygen consumption measurements suggest that the triple fnr mutant has higher respiratory activity when compared to the wild type, which we believe to be responsible for greater stability of the oxygen sensitive NifA protein. This conclusion was further substantiated by measuring the levels of NifA protein and its activity in fnr deletion strains in comparison with the wild-type. Fnr proteins are indirectly involved in controlling the activity of NifA in H. seropedicae, probably as a consequence of their influence on respiratory activity in relation to oxygen availability. Additionally we can suggest that there is some redundancy in the physiological function of the three Fnr paralogs in this organism, since altered respiration and effects on NifA activity are only observed in deletion strains lacking both fnr1 and fnr3.

Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer.

Science.gov (United States)

Curatti, Leonardo; Rubio, Luis M

2014-08-01

Some regions of the developing world suffer low cereal production yields due to low fertilizer inputs, among other factors. Biological N2 fixation, catalyzed by the prokaryotic enzyme nitrogenase, is an alternative to the use of synthetic N fertilizers. The molybdenum nitrogenase is an O2-labile metalloenzyme composed of the NifDK and NifH proteins, which biosyntheses require a number of nif gene products. A challenging strategy to increase cereal crop productivity in a scenario of low N fertilization is the direct transfer of nif genes into cereals. The sensitivity of nitrogenase to O2 and the apparent complexity of nitrogenase biosynthesis are the main barriers identified so far. Expression of active NifH requires the products of nifM, nifH, and possibly nifU and nifS, whereas active NifDK requires the products of nifH, nifD, nifK, nifB, nifE, nifN, and possibly nifU, nifS, nifQ, nifV, nafY, nifW and nifZ. Plastids and mitochondria are potential subcellular locations for nitrogenase. Both could provide the ATP and electrons required for nitrogenase to function but they differ in their internal O2 levels and their ability to incorporate ammonium into amino acids. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Laser Science and Technology Program Annual Report-2002 NIF Programs Directorate

International Nuclear Information System (INIS)

Hackel, L; Chen, H L

2003-01-01

The Laser Science and Technology (LSandT) Program's mission is to develop advanced lasers, optics, materials technologies, and applications to solve problems and create new capabilities of importance to the nation and the Laboratory. A top, near-term priority is to provide technical support in the deployment and upgrade of the National Ignition Facility (NIF). Our other program activities synergistically develop technologies that are consistent with the goals of the NIF Directorate and develop state-of-the-art capabilities. The primary objectives of LSandT activities in 2002 have been fourfold--(a) to support deployment of hardware and to enhance laser and optics performance for NIF, (b) to develop high-energy petawatt laser science and technology for the Department of Energy (DOE), (c) to develop advanced solid-state laser systems and optical components for the Department of Defense (DoD), and (d) to invent, develop, and deliver improved concepts and hardware for other government agencies and industry. LSandT activities during 2002 focused on seven major areas: (1) NIF Project-LSandT led major advances in the deployment of NIF Final Optics Assembly (FOA) and the development of 30.1 optics processing and treatment technologies to enhance NIF's operations and performance capabilities. (2) Stockpile Stewardship Program (SSP)-LSandT personnel continued development of ultrashort-pulse lasers and high-power, large-aperture optics for applications in SSP, extreme-field science and national defense. To enhance the high-energy petawatt (HEPW) capability in NIF, LSandT continued development of advanced compressor-grating and front-end laser technologies utilizing optical-parametric chirped-pulse amplification (OPCPA). (3) High-energy-density physics and inertial fusion energy-LSandT continued development of kW- to MW-class, diode-pumped, solid-state laser (DPSSL). (4) Department of Defense (DoD)-LSandT continued development of a 100 kw-class solid-state heat-capacity laser

Oxygen sensitivity of the nifLA promoter of Klebsiella pneumoniae.

OpenAIRE

Kong, Q T; Wu, Q L; Ma, Z F; Shen, S C

1986-01-01

Oxygen sensitivity of the nifLA promoter of Klebsiella pneumoniae has been demonstrated. Studies on the oxygen regulation of nifB-lacZ and nifH-lacZ fusions in the presence of the nifLA operon, which contains either an intact or a deleted nifL gene, indicate that possibly both the nifL promoter and the nifL product are responsible for nif repression by oxygen.

Development of IFE target systems on the NIF

International Nuclear Information System (INIS)

Schultz, K.R.; Fagaly, R.L.; Bernat, T.; Meier, W.; Petzoldt, R.; Foreman, L.

1995-01-01

The Target Systems session of the Workshop on NIF Experiments for IFE developed a list of critical issues for inertial fusion energy (IFE) target systems, and considered the potential of the National Ignition Facility (NIF) to help in the resolution of these issues and in the development of IFE target systems. This paper describes the IFE Target System issues, categorized into target fabrication issues and target transport issues, describes potential NIF IFE target systems experiments, considers the impact of these experiments on the NIF and discusses the development required before these experiments could be done. Most target systems issues must be resolved by development in the laboratory, not in the NIF, and some must be resolved before the NIF can be successful. However, experiments done in the NIF could play a valuable role in developing target systems for IFE. These experiments should have modest impact on the basic design of the NIF, but could require several hundred dedicated, high yield shots

Research Performance Progress Report: Diverging Supernova Explosion Experiments on NIF

Energy Technology Data Exchange (ETDEWEB)

Plewa, Tomasz [Florida State Univ., Tallahassee, FL (United States)

2016-10-25

The aim of this project was to design a series of blast-wave driven Rayleigh-Taylor (RT) experiments on the National Ignition Facility (NIF). The experiments of this kind are relevant to mixing in core-collapse supernovae (ccSNe) and have the potential to address previously unanswered questions in high-energy density physics (HEDP) and astrophysics. The unmatched laser power of the NIF laser offers a unique chance to observe and study “new physics” like the mass extensions observed in HEDP RT experiments performed on the Omega laser [1], which might be linked to self-generated magnetic fields [2] and so far could not be reproduced by numerical simulations. Moreover, NIF is currently the only facility that offers the possibility to execute a diverging RT experiment, which would allow to observe processes such as inter-shell penetration via turbulent mixing and shock-proximity effects (distortion of the shock by RT spikes).

Intragenic complementation by the nifJ-coded protein of Klebsiella pneumoniae.

OpenAIRE

Stacey, G; Zhu, J; Shah, V K; Shen, S C; Brill, W J

1982-01-01

A single mutation, nifC1005 (Jin et al. Sci. Sin. 23:108-118, 1980), located between nifH and nifJ in the nif cluster of Klebsiella pneumoniae, genetically complemented mutations in each of the 17 known nif genes. This suggested that the mutation is located in a new nif gene. We showed by complementation analyses that only 3 of 12 nifJ mutations tested were complemented by nifC1005. Nitrogenase activity in cell extracts of the mutant with nifC1005 as well as NifJ- mutants was stimulated by th...

Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool.

Science.gov (United States)

Grabbe, Roman; Schmitz, Ruth A

2003-04-01

In Klebsiella pneumoniae, the flavoprotein, NifL regulates NifA mediated transcriptional activation of the N2-fixation (nif) genes in response to molecular O2 and ammonium. We investigated the influence of membrane-bound oxidoreductases on nif-regulation by biochemical analysis of purified NifL and by monitoring NifA-mediated expression of nifH'-'lacZ reporter fusions in different mutant backgrounds. NifL-bound FAD-cofactor was reduced by NADH only in the presence of a redox-mediator or inside-out vesicles derived from anaerobically grown K. pneumoniae cells, indicating that in vivo NifL is reduced by electrons derived from membrane-bound oxidoreductases of the anaerobic respiratory chain. This mechanism is further supported by three lines of evidence: First, K. pneumoniae strains carrying null mutations of fdnG or nuoCD showed significantly reduced nif-induction under derepressing conditions, indicating that NifL inhibition of NifA was not relieved in the absence of formate dehydrogenase-N or NADH:ubiquinone oxidoreductase. The same effect was observed in a heterologous Escherichia coli system carrying a ndh null allele (coding for NADH dehydrogenaseII). Second, studying nif-induction in K. pneumoniae revealed that during anaerobic growth in glycerol, under nitrogen-limitation, the presence of the terminal electron acceptor nitrate resulted in a significant decrease of nif-induction. The final line of evidence is that reduced quinone derivatives, dimethylnaphthoquinol and menadiol, are able to transfer electrons to the FAD-moiety of purified NifL. On the basis of these data, we postulate that under anaerobic and nitrogen-limited conditions, NifL inhibition of NifA activity is relieved by reduction of the FAD-cofactor by electrons derived from the reduced quinone pool, generated by anaerobic respiration, that favours membrane association of NifL. We further hypothesize that the quinol/quinone ratio is important for providing the signal to NifL.

Structural and phylogenetic analysis of Rhodobacter capsulatus NifF: uncovering general features of nitrogen-fixation (nif)-flavodoxins.

Science.gov (United States)

Pérez-Dorado, Inmaculada; Bortolotti, Ana; Cortez, Néstor; Hermoso, Juan A

2013-01-09

Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50's loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as "short-chain" with the firmicutes flavodoxins and the "long-chain" with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.

NIF Discovery Science Eagle Nebula

Science.gov (United States)

Kane, Jave; Martinez, David; Pound, Marc; Heeter, Robert; Casner, Alexis; Villette, Bruno; Mancini, Roberto

2017-10-01

The University of Maryland and and LLNL are investigating the origin and dynamics of the famous Pillars of the Eagle Nebula and similar parsec-scale structures at the boundaries of HII regions in molecular hydrogen clouds. The National Ignition Facility (NIF) Discovery Science program Eagle Nebula has performed NIF shots to study models of pillar formation. The shots feature a new long-duration x-ray source, in which multiple hohlraums mimicking a cluster of stars are driven with UV light in series for 10 to 15 ns each to create a 30 to 60 ns output x-ray pulse. The source generates deeply nonlinear hydrodynamics in the Eagle science package, a structure of dense plastic and foam mocking up a molecular cloud containing a dense core. Omega EP and NIF shots have validated the source concept, showing that earlier hohlraums do not compromise later ones by preheat or by ejecting ablated plumes that deflect later beams. The NIF shots generated radiographs of shadowing-model pillars, and also showed evidence that cometary structures can be generated. The velocity and column density profiles of the NIF shadowing and cometary pillars have been compared with observations of the Eagle Pillars made at the millimeter-wave BIMA and CARMA observatories. Prepared by LLNL under Contract DE-AC52-07NA27344.

NIF: IFE applications, waste management and environmental impacts

International Nuclear Information System (INIS)

Lazaro, M.A.; Kirchner, F.R.; Miley, G.H.; Petra, M.

1996-01-01

Although many energy sources have been suggested for the future, inertial confinement fusion (ICF) has been demonstrated as scientifically feasible and deserves support for continued development. The National Ignition Facility (NIF), proposed by US DOE, is a next step in that direction. NIF would use ICF technology to achieve ignition and energy gain that would allow the development and continued support of national security and other civilian applications including inertial fusion energy power plants. NIF would also guarantee US leadership in dense plasma research. Four sites are being considered for NIF: LLNL, Los Alamos, Sandia, and two NTS sites. An environmental evaluation was performed which considered all impacts. This paper provides the results of the waste management analyses conducted on the proposed NIF sites. Overall, the proposed construction and operation of NIF should qualify it as a low-hazard, non-nuclear radiological facility with minor onsite and negligible offsite environmental impacts

Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif-Flavodoxins

Directory of Open Access Journals (Sweden)

Inmaculada Pérez-Dorado

2013-01-01

Full Text Available Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50’s loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as “short-chain” with the firmicutes flavodoxins and the “long-chain” with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.

The nif Gene Operon of the Methanogenic Archaeon Methanococcus maripaludis

Science.gov (United States)

Kessler, Peter S.; Blank, Carrine; Leigh, John A.

1998-01-01

Nitrogen fixation occurs in two domains, Archaea and Bacteria. We have characterized a nif (nitrogen fixation) gene cluster in the methanogenic archaeon Methanococcus maripaludis. Sequence analysis revealed eight genes, six with sequence similarity to known nif genes and two with sequence similarity to glnB. The gene order, nifH, ORF105 (similar to glnB), ORF121 (similar to glnB), nifD, nifK, nifE, nifN, and nifX, was the same as that found in part in other diazotrophic methanogens and except for the presence of the glnB-like genes, also resembled the order found in many members of the Bacteria. Using transposon insertion mutagenesis, we determined that an 8-kb region required for nitrogen fixation corresponded to the nif gene cluster. Northern analysis revealed the presence of either a single 7.6-kb nif mRNA transcript or 10 smaller mRNA species containing portions of the large transcript. Polar effects of transposon insertions demonstrated that all of these mRNAs arose from a single promoter region, where transcription initiated 80 bp 5′ to nifH. Distinctive features of the nif gene cluster include the presence of the six primary nif genes in a single operon, the placement of the two glnB-like genes within the cluster, the apparent physical separation of the cluster from any other nif genes that might be in the genome, the fragmentation pattern of the mRNA, and the regulation of expression by a repression mechanism described previously. Our study and others with methanogenic archaea reporting multiple mRNAs arising from gene clusters with only a single putative promoter sequence suggest that mRNA processing following transcription may be a common occurrence in methanogens. PMID:9515920

Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB

Directory of Open Access Journals (Sweden)

Simon Arragain

2017-11-01

Full Text Available One of the main hurdles to engineer nitrogenase in a non-diazotrophic host is achieving NifB activity. NifB is an extremely unstable and oxygen sensitive protein that catalyzes a low-potential SAM-radical dependent reaction. The product of NifB activity is called NifB-co, a complex [8Fe-9S-C] cluster that serves as obligate intermediate in the biosyntheses of the active-site cofactors of all known nitrogenases. Here we study the diversity and phylogeny of naturally occurring NifB proteins, their protein architecture and the functions of the distinct NifB domains in order to understand what defines a catalytically active NifB. Focus is on NifB from the thermophile Chlorobium tepidum (two-domain architecture, the hyperthermophile Methanocaldococcus infernus (single-domain architecture and the mesophile Klebsiella oxytoca (two-domain architecture, showing in silico characterization of their nitrogen fixation (nif gene clusters, conserved NifB motifs, and functionality. C. tepidum and M. infernus NifB were able to complement an Azotobacter vinelandii (ΔnifB mutant restoring the Nif+ phenotype and thus demonstrating their functionality in vivo. In addition, purified C. tepidum NifB exhibited activity in the in vitro NifB-dependent nitrogenase reconstitution assay. Intriguingly, changing the two-domain K. oxytoca NifB to single-domain by removal of the C-terminal NifX-like extension resulted in higher in vivo nitrogenase activity, demonstrating that this domain is not required for nitrogen fixation in mesophiles.

Expression of the nifA gene of Herbaspirillum seropedicae: role of the NtrC and NifA binding sites and of the -24/-12 promoter element.

Science.gov (United States)

Souza, E M; Pedrosa, F O; Rigo, L U; Machado, H B; Yates, M G

2000-06-01

The nifA promoter of Herbaspirillum seropedicae contains potential NtrC, NifA and IHF binding sites together with a -12/-24 sigma(N)-dependent promoter. This region has now been investigated by deletion mutagenesis for the effect of NtrC and NifA on the expression of a nifA::lacZ fusion. A 5' end to the RNA was identified at position 641, 12 bp downstream from the -12/-24 promoter. Footprinting experiments showed that the G residues at positions -26 and -9 are hypermethylated, and that the region from -10 to +10 is partially melted under nitrogen-fixing conditions, confirming that this is the active nifA promoter. In H. seropedicae nifA expression from the sigma(N)-dependent promoter is repressed by fixed nitrogen but not by oxygen and is probably activated by the NtrC protein. NifA protein is apparently not essential for nifA expression but it can still bind the NifA upstream activating sequence.

Fault tolerance of the NIF power conditioning system

International Nuclear Information System (INIS)

Larson, D.W.; Anderson, R.; Boyes, J.

1995-01-01

The tolerance of the circuit topology proposed for the National Ignition Facility (NIF) power conditioning system to specific fault conditions is investigated. A new pulsed power circuit is proposed for the NIF which is simpler and less expensive than previous ICF systems. The inherent fault modes of the new circuit are different from the conventional approach, and must be understood to ensure adequate NIF system reliability. A test-bed which simulates the NIF capacitor module design was constructed to study the circuit design. Measurements from test-bed experiments with induced faults are compared with results from a detailed circuit model. The model is validated by the measurements and used to predict the behavior of the actual NIF module during faults. The model can be used to optimize fault tolerance of the NIF module through an appropriate distribution of circuit inductance and resistance. The experimental and modeling results are presented, and fault performance is compared with the ratings of pulsed power components. Areas are identified which require additional investigation

Environment-Dependent Distribution of the Sediment nifH-Harboring Microbiota in the Northern South China Sea

Science.gov (United States)

Yang, Jinying; Li, Jing; Luan, Xiwu; Zhang, Yunbo; Gu, Guizhou; Xue, Rongrong; Zong, Mingyue; Klotz, Martin G.

2013-01-01

The South China Sea (SCS), the largest marginal sea in the Western Pacific Ocean, is a huge oligotrophic water body with very limited influx of nitrogenous nutrients. This suggests that sediment microbial N2 fixation plays an important role in the production of bioavailable nitrogen. To test the molecular underpinning of this hypothesis, the diversity, abundance, biogeographical distribution, and community structure of the sediment diazotrophic microbiota were investigated at 12 sampling sites, including estuarine, coastal, offshore, deep-sea, and methane hydrate reservoirs or their prospective areas by targeting nifH and some other functional biomarker genes. Diverse and novel nifH sequences were obtained, significantly extending the evolutionary complexity of extant nifH genes. Statistical analyses indicate that sediment in situ temperature is the most significant environmental factor influencing the abundance, community structure, and spatial distribution of the sediment nifH-harboring microbial assemblages in the northern SCS (nSCS). The significantly positive correlation of the sediment pore water NH4+ concentration with the nifH gene abundance suggests that the nSCS sediment nifH-harboring microbiota is active in N2 fixation and NH4+ production. Several other environmental factors, including sediment pore water PO43− concentration, sediment organic carbon, nitrogen and phosphorus levels, etc., are also important in influencing the community structure, spatial distribution, or abundance of the nifH-harboring microbial assemblages. We also confirmed that the nifH genes encoded by archaeal diazotrophs in the ANME-2c subgroup occur exclusively in the deep-sea methane seep areas, providing for the possibility to develop ANME-2c nifH genes as a diagnostic tool for deep-sea methane hydrate reservoir discovery. PMID:23064334

Role of conserved cysteine residues in Herbaspirillum seropedicae NifA activity.

Science.gov (United States)

Oliveira, Marco A S; Baura, Valter A; Aquino, Bruno; Huergo, Luciano F; Kadowaki, Marco A S; Chubatsu, Leda S; Souza, Emanuel M; Dixon, Ray; Pedrosa, Fábio O; Wassem, Roseli; Monteiro, Rose A

2009-01-01

Herbaspirillum seropedicae is an endophytic diazotrophic bacterium that associates with economically important crops. NifA protein, the transcriptional activator of nif genes in H. seropedicae, binds to nif promoters and, together with RNA polymerase-sigma(54) holoenzyme, catalyzes the formation of open complexes to allow transcription initiation. The activity of H. seropedicae NifA is controlled by ammonium and oxygen levels, but the mechanisms of such control are unknown. Oxygen sensitivity is attributed to a conserved motif of cysteine residues in NifA that spans the central AAA+ domain and the interdomain linker that connects the AAA+ domain to the C-terminal DNA binding domain. Here we mutagenized this conserved motif of cysteines and assayed the activity of mutant proteins in vivo. We also purified the mutant variants of NifA and tested their capacity to bind to the nifB promoter region. Chimeric proteins between H. seropedicae NifA, an oxygen-sensitive protein, and Azotobacter vinelandii NifA, an oxygen-tolerant protein, were constructed and showed that the oxygen response is conferred by the central AAA+ and C-terminal DNA binding domains of H. seropedicae NifA. We conclude that the conserved cysteine motif is essential for NifA activity, although single cysteine-to-serine mutants are still competent at binding DNA.

The National Ignition Facility (NIF) as a User Facility

Science.gov (United States)

Keane, Christopher; NIF Team

2013-10-01

The National Ignition Facility (NIF) has made significant progress towards operation as a user facility. Through June 2013, NIF conducted over 1200 experiments in support of ICF, HED science, and development of facility capabilities. The NIF laser has met or achieved all specifications and a wide variety of diagnostic and target fabrication capabilities are in place. A NIF User Group and associated Executive Board have been formed. Two User Group meetings have been conducted since formation of the User Group. NIF experiments in fundamental science have provided important new results. NIF ramp compression experiments have been conducted using diamond and iron, with EOS results obtained at pressures up to approximately 50 Mbar and 8 Mbar, respectively. Initial experiments in supernova hydrodynamics, the fundamental physics of the Rayleigh-Taylor instability, and equation of state in the Gbar pressure regime have also been conducted. This presentation will discuss the fundamental science program at NIF, including the proposal solicitation and scientific review processes and other aspects of user facility operation. This work was performed under the auspices of the Lawrence Livermore National Security, LLC, (LLNS) under Contract No. DE-AC52-07NA27344.

NIFS symposium: toward the research of fusion burning plasmas

International Nuclear Information System (INIS)

Itoh, Sanae

1993-07-01

NIFS symposium, entitled 'Toward the research of Fusion Burning Plasmas - Present status and Future Strategy' was held at NIFS on July 15th 1992. This NIFS symposium covers various topics related to burning plasma, e.g., JET DT experiment, Plan for DT experiment on TFTR as well as the future trends among researchers. To study the critical issues and trends of future research, a questionnaire was sent to about 100 researchers. This report presents such activities in the NIFS symposium. (author)

NIF total neutron yield diagnostic

International Nuclear Information System (INIS)

Cooper, Gary W.; Ruiz, Carlos L.

2001-01-01

We have designed a total neutron yield diagnostic for the National Ignition Facility (NIF) which is based on the activation of In and Cu samples. The particular approach that we have chosen is one in which we calibrate the entire counting system and which we call the ''F factor'' method. In this method, In and/or Cu samples are exposed to known sources of DD and DT neutrons. The activated samples are then counted with an appropriate system: a high purity Ge detector for In and a NaI coincidence system for Cu. We can then calculate a calibration factor, which relates measured activity to total neutron yield. The advantage of this approach is that specific knowledge of such quantities as cross sections and detector efficiencies is not needed. Unless the actual scattering environment of the NIF can be mocked up in the calibration experiment, the F factor will have to be modified using the results of a numerical simulation of the NIF scattering environment. In this article, the calibration factor methodology will be discussed and experimental results for the calibration factors will be presented. Total NIF neutron yields of 10 9 --10 19 can be measured with this method assuming a 50 cm stand-off distance can be employed for the lower yields

At the interface of the auditory and vocal motor systems: NIf and its role in vocal processing, production and learning.

Science.gov (United States)

Lewandowski, Brian; Vyssotski, Alexei; Hahnloser, Richard H R; Schmidt, Marc

2013-06-01

Communication between auditory and vocal motor nuclei is essential for vocal learning. In songbirds, the nucleus interfacialis of the nidopallium (NIf) is part of a sensorimotor loop, along with auditory nucleus avalanche (Av) and song system nucleus HVC, that links the auditory and song systems. Most of the auditory information comes through this sensorimotor loop, with the projection from NIf to HVC representing the largest single source of auditory information to the song system. In addition to providing the majority of HVC's auditory input, NIf is also the primary driver of spontaneous activity and premotor-like bursting during sleep in HVC. Like HVC and RA, two nuclei critical for song learning and production, NIf exhibits behavioral-state dependent auditory responses and strong motor bursts that precede song output. NIf also exhibits extended periods of fast gamma oscillations following vocal production. Based on the converging evidence from studies of physiology and functional connectivity it would be reasonable to expect NIf to play an important role in the learning, maintenance, and production of song. Surprisingly, however, lesions of NIf in adult zebra finches have no effect on song production or maintenance. Only the plastic song produced by juvenile zebra finches during the sensorimotor phase of song learning is affected by NIf lesions. In this review, we carefully examine what is known about NIf at the anatomical, physiological, and behavioral levels. We reexamine conclusions drawn from previous studies in the light of our current understanding of the song system, and establish what can be said with certainty about NIf's involvement in song learning, maintenance, and production. Finally, we review recent theories of song learning integrating possible roles for NIf within these frameworks and suggest possible parallels between NIf and sensorimotor areas that form part of the neural circuitry for speech processing in humans. Copyright © 2013 Elsevier

Endophytic Herbaspirillum seropedicae expresses nif genes in gramineous plants.

Science.gov (United States)

Roncato-Maccari, Lauren D B; Ramos, Humberto J O; Pedrosa, Fabio O; Alquini, Yedo; Chubatsu, Leda S; Yates, Marshall G; Rigo, Liu U; Steffens, Maria Berenice R; Souza, Emanuel M

2003-07-01

Abstract The interactions between maize, sorghum, wheat and rice plants and Herbaspirillum seropedicae were examined microscopically following inoculation with the H. seropedicae LR15 strain, a Nif(+) (Pnif::gusA) mutant obtained by the insertion of a gusA-kanamycin cassette into the nifH gene of the H. seropedicae wild-type strain. The expression of the Pnif::gusA fusion was followed during the association of the diazotroph with the gramineous species. Histochemical analysis of seedlings of maize, sorghum, wheat and rice grown in vermiculite showed that strain LR15 colonized root surfaces and inner tissues. In early steps of the endophytic association, H. seropedicae colonized root exudation sites, such as axils of secondary roots and intercellular spaces of the root cortex; it then occupied the vascular tissue and there expressed nif genes. The expression of nif genes occurred in roots, stems and leaves as detected by the GUS reporter system. The expression of nif genes was also observed in bacterial colonies located in the external mucilaginous root material, 8 days after inoculation. Moreover, the colonization of plant tissue by H. seropedicae did not depend on the nitrogen-fixing ability, since similar numbers of cells were isolated from roots or shoots of the plants inoculated with Nif(+) or Nif(-) strains.

NIF-Scale Hohlraum Asymmetry Studies Using Point-Projection Radiograph of Thin Shells

International Nuclear Information System (INIS)

Pollaine, S.; Bradley, D.; Landen, O.; Wallace, R.; Jones, O.

2000-01-01

Our current OMEGA experimental campaign is developing the thin shell diagnostic for use on NIF with the needed accuracy. The thin shell diagnostic has the advantage of linearity over alternative measurement techniques, so that low-order modes will not corrupt the measurement of high-order modes. Although our random measurement errors are adequate, we need to monitor beam balance and ensure that the thin shells have a uniform thickness

Behavioral Model of High Performance Camera for NIF Optics Inspection

International Nuclear Information System (INIS)

Hackel, B M

2007-01-01

The purpose of this project was to develop software that will model the behavior of the high performance Spectral Instruments 1000 series Charge-Coupled Device (CCD) camera located in the Final Optics Damage Inspection (FODI) system on the National Ignition Facility. NIF's target chamber will be mounted with 48 Final Optics Assemblies (FOAs) to convert the laser light from infrared to ultraviolet and focus it precisely on the target. Following a NIF shot, the optical components of each FOA must be carefully inspected for damage by the FODI to ensure proper laser performance during subsequent experiments. Rapid image capture and complex image processing (to locate damage sites) will reduce shot turnaround time; thus increasing the total number of experiments NIF can conduct during its 30 year lifetime. Development of these rapid processes necessitates extensive offline software automation -- especially after the device has been deployed in the facility. Without access to the unique real device or an exact behavioral model, offline software testing is difficult. Furthermore, a software-based behavioral model allows for many instances to be running concurrently; this allows multiple developers to test their software at the same time. Thus it is beneficial to construct separate software that will exactly mimic the behavior and response of the real SI-1000 camera

Optimization of the NIF ignition point design hohlraum

International Nuclear Information System (INIS)

Callahan, D A; Hinkel, D E; Berger, R L; Divol, L; Dixit, S N; Edwards, M J; Haan, S W; Jones, O S; Lindl, J D; Meezan, N B; Michel, P A; Pollaine, S M; Suter, L J; Town, R P J; Bradley, P A

2008-01-01

In preparation for the start of NIF ignition experiments, we have designed a porfolio of targets that span the temperature range that is consistent with initial NIF operations: 300 eV, 285 eV, and 270 eV. Because these targets are quite complicated, we have developed a plan for choosing the optimum hohlraum for the first ignition attempt that is based on this portfolio of designs coupled with early NIF experiements using 96 beams. These early experiments will measure the laser plasma instabilities of the candidate designs and will demonstrate our ability to tune symmetry in these designs. These experimental results, coupled with the theory and simulations that went into the designs, will allow us to choose the optimal hohlraum for the first NIF ignition attempt

Optimization of the NIF ignition point design hohlraum

Science.gov (United States)

Callahan, D. A.; Hinkel, D. E.; Berger, R. L.; Divol, L.; Dixit, S. N.; Edwards, M. J.; Haan, S. W.; Jones, O. S.; Lindl, J. D.; Meezan, N. B.; Michel, P. A.; Pollaine, S. M.; Suter, L. J.; Town, R. P. J.; Bradley, P. A.

2008-05-01

In preparation for the start of NIF ignition experiments, we have designed a porfolio of targets that span the temperature range that is consistent with initial NIF operations: 300 eV, 285 eV, and 270 eV. Because these targets are quite complicated, we have developed a plan for choosing the optimum hohlraum for the first ignition attempt that is based on this portfolio of designs coupled with early NIF experiements using 96 beams. These early experiments will measure the laser plasma instabilities of the candidate designs and will demonstrate our ability to tune symmetry in these designs. These experimental results, coupled with the theory and simulations that went into the designs, will allow us to choose the optimal hohlraum for the first NIF ignition attempt.

NIF Maintenance Plan March 2011

Energy Technology Data Exchange (ETDEWEB)

Van Wonterghem, Bruno M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2014-01-30

Ensuring the reliability of the NIF, including its support systems and utilities, is essential to ensuring the availability of the NIF in its support of laser operations. This document identifies the policies and procedures necessary to perform and support the maintenance of the NIF’s systems. These systems are all encompassing and include the facility, beampath, Line Replaceable Units (LRUs), Safety Interlock System (SIS), and diagnostics and utilities that create the environments within the beampath consisting of vacuum, argon, or clean dry air.

NIF Ambient Vibration Measurements

International Nuclear Information System (INIS)

Noble, C.R.; Hoehler, M.S.; S.C. Sommer

1999-01-01

LLNL has an ongoing research and development project that includes developing data acquisition systems with remote wireless communication for monitoring the vibrations of large civil engineering structures. In order to establish the capability of performing remote sensing over an extended period of time, the researchers needed to apply this technology to a real structure. The construction of the National Ignition Facility provided an opportunity to test the data acquisition system on a large structure to monitor whether the facility is remaining within the strict ambient vibration guidelines. This document will briefly discuss the NIF ambient vibration requirements and summarize the vibration measurements performed during the Spring and Summer of 1999. In addition, a brief description of the sensors and the data acquisition systems will be provided in Appendix B

NIF ICCS network design and loading analysis

International Nuclear Information System (INIS)

Tietbohl, G; Bryant, R

1998-01-01

The National Ignition Facility (NIF) is housed within a large facility about the size of two football fields. The Integrated Computer Control System (ICCS) is distributed throughout this facility and requires the integration of about 40,000 control points and over 500 video sources. This integration is provided by approximately 700 control computers distributed throughout the NIF facility and a network that provides the communication infrastructure. A main control room houses a set of seven computer consoles providing operator access and control of the various distributed front-end processors (FEPs). There are also remote workstations distributed within the facility that allow provide operator console functions while personnel are testing and troubleshooting throughout the facility. The operator workstations communicate with the FEPs which implement the localized control and monitoring functions. There are different types of FEPs for the various subsystems being controlled. This report describes the design of the NIF ICCS network and how it meets the traffic loads that will are expected and the requirements of the Sub-System Design Requirements (SSDR's). This document supersedes the earlier reports entitled Analysis of the National Ignition Facility Network, dated November 6, 1996 and The National Ignition Facility Digital Video and Control Network, dated July 9, 1996. For an overview of the ICCS, refer to the document NIF Integrated Computer Controls System Description (NIF-3738)

Mini-chamber, an advanced protection concept for NIF

International Nuclear Information System (INIS)

Peterson, P.F.; Scott, J.M.

1996-01-01

Inertial confinement fusion (ICF) target debris and ablated near-target materials pose the primary threat to the National Ignition Facility (NIF) final optics debris shields, as well as a major challenge in future inertial fusion energy (IFE) power plants. This work discusses a NIF 'mini-chamber,' designed to mitigate the debris threat. Although the NIF base-line design protects against debris using a frost-protected target positioner and refractory first-wall coatings, the mini-chamber provides important flexibility in three areas: debris-shield protection from beyond-design basis shots (i.e. heavy hohlraums, special diagnostics, shields); fielding of large experiments with significant surface ablation; and studying key ablation and gas-dynamics issues for liquid-wall IFE power plants. Key mini-chamber modeling results are presented, followed by discussion of equipment requirements for fielding a NIF mini-chamber. 7 refs., 3 figs

Analysis of phylogeny and codon usage bias and relationship of GC content, amino acid composition with expression of the structural nif genes.

Science.gov (United States)

Mondal, Sunil Kanti; Kundu, Sudip; Das, Rabindranath; Roy, Sujit

2016-08-01

Bacteria and archaea have evolved with the ability to fix atmospheric dinitrogen in the form of ammonia, catalyzed by the nitrogenase enzyme complex which comprises three structural genes nifK, nifD and nifH. The nifK and nifD encodes for the beta and alpha subunits, respectively, of component 1, while nifH encodes for component 2 of nitrogenase. Phylogeny based on nifDHK have indicated that Cyanobacteria is closer to Proteobacteria alpha and gamma but not supported by the tree based on 16SrRNA. The evolutionary ancestor for the different trees was also different. The GC1 and GC2% analysis showed more consistency than GC3% which appeared to below for Firmicutes, Cyanobacteria and Euarchaeota while highest in Proteobacteria beta and clearly showed the proportional effect on the codon usage with a few exceptions. Few genes from Firmicutes, Euryarchaeota, Proteobacteria alpha and delta were found under mutational pressure. These nif genes with low and high GC3% from different classes of organisms showed similar expected number of codons. Distribution of the genes and codons, based on codon usage demonstrated opposite pattern for different orientation of mirror plane when compared with each other. Overall our results provide a comprehensive analysis on the evolutionary relationship of the three structural nif genes, nifK, nifD and nifH, respectively, in the context of codon usage bias, GC content relationship and amino acid composition of the encoded proteins and exploration of crucial statistical method for the analysis of positive data with non-constant variance to identify the shape factors of codon adaptation index.

Controlled expression of nif and isc iron-sulfur protein maturation components reveals target specificity and limited functional replacement between the two systems.

Science.gov (United States)

Dos Santos, Patricia C; Johnson, Deborah C; Ragle, Brook E; Unciuleac, Mihaela-Carmen; Dean, Dennis R

2007-04-01

The nitrogen-fixing organism Azotobacter vinelandii contains at least two systems that catalyze formation of [Fe-S] clusters. One of these systems is encoded by nif genes, whose products supply [Fe-S] clusters required for maturation of nitrogenase. The other system is encoded by isc genes, whose products are required for maturation of [Fe-S] proteins that participate in general metabolic processes. The two systems are similar in that they include an enzyme for the mobilization of sulfur (NifS or IscS) and an assembly scaffold (NifU or IscU) upon which [Fe-S] clusters are formed. Normal cellular levels of the Nif system, which supplies [Fe-S] clusters for the maturation of nitrogenase, cannot also supply [Fe-S] clusters for the maturation of other cellular [Fe-S] proteins. Conversely, when produced at the normal physiological levels, the Isc system cannot supply [Fe-S] clusters for the maturation of nitrogenase. In the present work we found that such target specificity for IscU can be overcome by elevated production of NifU. We also found that NifU, when expressed at normal levels, is able to partially replace the function of IscU if cells are cultured under low-oxygen-availability conditions. In contrast to the situation with IscU, we could not establish conditions in which the function of IscS could be replaced by NifS. We also found that elevated expression of the Isc components, as a result of deletion of the regulatory iscR gene, improved the capacity for nitrogen-fixing growth of strains deficient in either NifU or NifS.

Tests and calibration of NIF neutron time of flight detectors.

Science.gov (United States)

Ali, Z A; Glebov, V Yu; Cruz, M; Duffy, T; Stoeckl, C; Roberts, S; Sangster, T C; Tommasini, R; Throop, A; Moran, M; Dauffy, L; Horsefield, C

2008-10-01

The National Ignition Facility (NIF) neutron time of flight (NTOF) diagnostic will measure neutron yield and ion temperature in all NIF campaigns in DD, DT, and THD(*) implosions. The NIF NTOF diagnostic is designed to measure neutron yield from 1x10(9) to 2x10(19). The NTOF consists of several detectors of varying sensitivity located on the NIF at about 5 and 20 m from the target. Production, testing, and calibration of the NIF NTOF detectors have begun at the Laboratory for Laser Energetics (LLE). Operational tests of the NTOF detectors were performed on several facilities including the OMEGA laser at LLE and the Titan laser at Lawrence Livermore National Laboratory. Neutron calibrations were carried out on the OMEGA laser. Results of the NTOF detector tests and calibration will be presented.

The presence of five nifH-like sequences in Clostridium pasteurianum: sequence divergence and transcription properties.

OpenAIRE

Wang, S Z; Chen, J S; Johnson, J L

1988-01-01

The nifH gene encodes the iron protein (component II) of the nitrogenase complex. We have previously shown the presence in Clostridium pasteurianum of two nifH-like sequences in addition to the nifH1 gene which codes for a protein identical to the isolated iron protein. In the present study, we report that there are at least five nifH-like sequences in C. pasteurianum. DNA sequencing data indicate that the six nifH (nifH1) and nifH-like (nifH2, nifH3, nifH4, nifH5 and nifH6) sequences are not...

Towards understanding the nitrogen signal transduction for nif gene expression in Klebsiella pneumoniae.

Science.gov (United States)

Glöer, Jens; Thummer, Robert; Ullrich, Heike; Schmitz, Ruth A

2008-12-01

In the diazotroph Klebsiella pneumoniae, the nitrogen sensory protein GlnK mediates the cellular nitrogen status towards the NifL/NifA system that regulates transcription of the nitrogen fixation genes in response to ammonium and molecular oxygen. To identify amino acids of GlnK essential for this signal transduction by protein-protein interaction, we performed random point mutagenesis by PCR amplification under conditions of reduced Taq polymerase fidelity. Three thousand two hundred mutated glnK genes were screened to identify those that would no longer complement a K. pneumoniaeDeltaglnK strain for growth under nitrogen fixing conditions. Twenty-four candidates resulting in a Nif(-) phenotype were identified, carrying 1-11 amino acid changes in GlnK. Based on these findings, as well as structural data, several single mutations were introduced into glnK by site-directed mutagenesis, and the Nif phenotype and the respective effects on NifA-mediated nif gene induction was monitored in K. pneumoniae using a chromosomal nifK'-'lacZ fusion. Single amino acid changes resulting in significant nif gene inhibition under nitrogen limiting conditions were located within the highly conserved T-loop (A43G, A49T and N54D), the body of the protein (G87V and K79E) and in the C-terminal region (I100M, R103S, E106Q and D108G). Complex formation analyses between GlnK (wild-type or derivatives) and NifL or NifA in response to 2-oxoglutarate indicated that: (a) besides the T-loop, the C-terminal region of GlnK is essential for the interaction with NifL and NifA and (b) GlnK binds both proteins in the absence of 2-oxoglutarate, whereas, in the presence of 2-oxoglutarate, NifA is released but NifL remains bound to GlnK.

Comparison of plastic, high-density carbon, and beryllium as NIF ablators

Science.gov (United States)

Kritcher, Andrea

2017-10-01

An effort is underway to compare the three principal ablators for National Ignition Facility (NIF) implosions: plastic (CH), High Density Carbon (HDC), and beryllium (Be). This presentation will summarize the comparison and discuss in more detail the issues pertaining to hohlraum performance and symmetry. Several aspects of the hohlraum design are affected by the ablator properties, as the ablator constrains the first shock and determines the overall pulse length. HDC targets can utilize shorter pulse lengths due to the thinner, higher density shell, and should be less susceptible to late time wall motion. However, HDC requires a larger picket energy to ensure adequate melt, leading to increased late time wall movement. Be is intermediate to CH and HDC in both these regards, and has more ablated material in the hohlraum. These tradeoffs as well as other design choices for currently fielded campaigns are assessed in this work. To assess consistently the radiation drive and symmetry, integrated postshot simulations of the hohlraum and capsule were done for each design using the same methodology. The simulation results are compared to experimental data. Using this post-shot model, we make a projection of the relative plausible performance that can be achieved, while maintaining adequate symmetry, using the full NIF laser, i.e. 1.8 MJ/500 TW Full NIF Equivalent (FNE). The hydrodynamic stability of the different ablators is also an important consideration and will be presented for the current platforms and projection to FNE. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Kinetics of Nif gene expression in a nitrogen-fixing bacterium.

Science.gov (United States)

Poza-Carrión, César; Jiménez-Vicente, Emilio; Navarro-Rodríguez, Mónica; Echavarri-Erasun, Carlos; Rubio, Luis M

2014-02-01

Nitrogen fixation is a tightly regulated trait. Switching from N2 fixation-repressing conditions to the N2-fixing state is carefully controlled in diazotrophic bacteria mainly because of the high energy demand that it imposes. By using quantitative real-time PCR and quantitative immunoblotting, we show here how nitrogen fixation (nif) gene expression develops in Azotobacter vinelandii upon derepression. Transient expression of the transcriptional activator-encoding gene, nifA, was followed by subsequent, longer-duration waves of expression of the nitrogenase biosynthetic and structural genes. Importantly, expression timing, expression levels, and NifA dependence varied greatly among the nif operons. Moreover, the exact concentrations of Nif proteins and their changes over time were determined for the first time. Nif protein concentrations were exquisitely balanced, with FeMo cofactor biosynthetic proteins accumulating at levels 50- to 100-fold lower than those of the structural proteins. Mutants lacking nitrogenase structural genes or impaired in FeMo cofactor biosynthesis showed overenhanced responses to derepression that were proportional to the degree of nitrogenase activity impairment, consistent with the existence of at least two negative-feedback regulatory mechanisms. The first such mechanism responded to the levels of fixed nitrogen, whereas the second mechanism appeared to respond to the levels of the mature NifDK component. Altogether, these findings provide a framework to engineer N2 fixation in nondiazotrophs.

Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense

Directory of Open Access Journals (Sweden)

L.M.P. Passaglia

1998-11-01

Full Text Available NifA protein activates transcription of nitrogen fixation operons by the alternative sigma54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST-NifA fusion protein and proteolytic release of GST yielded inactive and partially soluble NifA. However, the purified NifA was able to induce the production of specific anti-A. brasilense NifA-antiserum that recognized NifA from A. brasilense but not from K. pneumoniae. Both GST-NifA and NifA expressed from the E. coli tac promoter are able to activate transcription from the nifHDK promoter but only in an A. brasilense background. In order to investigate the mechanism that regulates NifA binding capacity we have used E. coli total protein extracts expressing A. brasilense nifA in mobility shift assays. DNA fragments carrying the two overlapping, wild-type or mutated UAS motifs present in the nifH promoter region revealed a retarded band of related size. These data show that the binding activity present in the C-terminal domain of A. brasilense NifA protein is still functional even in the presence of oxygen.

Sequencing and functional analysis of the nifENXorf1orf2 gene cluster of Herbaspirillum seropedicae.

Science.gov (United States)

Klassen, G; Pedrosa, F O; Souza, E M; Yates, M G; Rigo, L U

1999-12-01

A 5.1-kb DNA fragment from the nifHDK region of H. seropedicae was isolated and sequenced. Sequence analysis showed the presence of nifENXorf1orf2 but nifTY were not present. No nif or consensus promoter was identified. Furthermore, orf1 expression occurred only under nitrogen-fixing conditions and no promoter activity was detected between nifK and nifE, suggesting that these genes are expressed from the upstream nifH promoter and are parts of a unique nif operon. Mutagenesis studies indicate that nifN was essential for nitrogenase activity whereas nifXorf1orf2 were not. High homology between the C-terminal region of the NifX and NifB proteins from H. seropedicae was observed. Since the NifX and NifY proteins are important for FeMo cofactor (FeMoco) synthesis, we propose that alternative proteins with similar activities exist in H. seropedicae.

Configuring NIF for direct drive experiments

International Nuclear Information System (INIS)

Eimerl, D.; Rothenberg, J.; Key, M.

1995-01-01

The National Ignition Facility (NIF) is a proposed 1.8 MJ laser facility for carrying out experiments in inertial confinement fusion, currently designed for indirect drive experiments. The direct drive approach is being pursued at the 30 kJ Omega facility at the University of Rochester. In this paper we discuss the modifications to the NIF laser that would be required for both indirect and direct drive experiments. A primary concern is the additional cost of adding direct drive capability to the facility

NIF optical specifications - the importance of the RMS gradient specification

International Nuclear Information System (INIS)

Auerbach, J. M.; Cotton, C. T.; English, R. E.; Henesian, M. A.; Hunt, J. T.; Kelly, J. H.; Lawson, J. K.; Sacks, J. B.; Shoup, M. J.; Trenholme, W. H.

1998-01-01

The performance of the National Ignition Facility (NIF), especially in terms of laser focusability, will be determined by several key factors. One of these key factors is the optical specification for the thousands of large aperture optics that will comprise the 192 beamlines. We have previously reported on the importance of the specification of the power spectral density (PSD) on NIF performance. Recently, we have been studying the importance of long spatial wavelength (>33 mm) phase errors on focusability. We have concluded that the preferred metric for determining the impact of these long spatial wavelength phase errors is the rms phase gradient. In this paper, we outline the overall approach to NIF optical specifications, detail the impact of the rms phase gradient on NIF focusability, discuss its trade-off with the PSD in determining the spot size and review measurements of optics similar to those to be manufactured for NIF

Effect of point mutations on Herbaspirillum seropedicae NifA activity

International Nuclear Information System (INIS)

Aquino, B.; Stefanello, A.A.; Oliveira, M.A.S.; Pedrosa, F.O.; Souza, E.M.; Monteiro, R.A.; Chubatsu, L.S.

2015-01-01

NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein can still activate nif genes transcription; however, ammonium regulation is lost. In this work, we generated eight constructs resulting in point mutations in H. seropedicae NifA and analyzed their effect on nifH transcription in Escherichia coli and H. seropedicae. Mutations K22V, T160E, M161V, L172R, and A215D resulted in inactive proteins. Mutations Q216I and S220I produced partially active proteins with activity control similar to wild-type NifA. However, mutation G25E, located in the GAF domain, resulted in an active protein that did not require GlnK for activity and was partially sensitive to ammonium. This suggested that G25E may affect the negative interaction between the N-terminal GAF domain and the catalytic central domain under high ammonium concentrations, thus rendering the protein constitutively active, or that G25E could lead to a conformational change comparable with that when GlnK interacts with the GAF domain

Effect of point mutations on Herbaspirillum seropedicae NifA activity

Directory of Open Access Journals (Sweden)

B. Aquino

2015-08-01

Full Text Available NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein can still activate nif genes transcription; however, ammonium regulation is lost. In this work, we generated eight constructs resulting in point mutations in H. seropedicae NifA and analyzed their effect on nifH transcription in Escherichia coli and H. seropedicae. Mutations K22V, T160E, M161V, L172R, and A215D resulted in inactive proteins. Mutations Q216I and S220I produced partially active proteins with activity control similar to wild-type NifA. However, mutation G25E, located in the GAF domain, resulted in an active protein that did not require GlnK for activity and was partially sensitive to ammonium. This suggested that G25E may affect the negative interaction between the N-terminal GAF domain and the catalytic central domain under high ammonium concentrations, thus rendering the protein constitutively active, or that G25E could lead to a conformational change comparable with that when GlnK interacts with the GAF domain.

Effect of point mutations on Herbaspirillum seropedicae NifA activity.

Science.gov (United States)

Aquino, B; Stefanello, A A; Oliveira, M A S; Pedrosa, F O; Souza, E M; Monteiro, R A; Chubatsu, L S

2015-08-01

NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein can still activate nif genes transcription; however, ammonium regulation is lost. In this work, we generated eight constructs resulting in point mutations in H. seropedicae NifA and analyzed their effect on nifH transcription in Escherichia coli and H. seropedicae. Mutations K22V, T160E, M161V, L172R, and A215D resulted in inactive proteins. Mutations Q216I and S220I produced partially active proteins with activity control similar to wild-type NifA. However, mutation G25E, located in the GAF domain, resulted in an active protein that did not require GlnK for activity and was partially sensitive to ammonium. This suggested that G25E may affect the negative interaction between the N-terminal GAF domain and the catalytic central domain under high ammonium concentrations, thus rendering the protein constitutively active, or that G25E could lead to a conformational change comparable with that when GlnK interacts with the GAF domain.

Effect of point mutations on Herbaspirillum seropedicae NifA activity

Energy Technology Data Exchange (ETDEWEB)

Aquino, B.; Stefanello, A.A.; Oliveira, M.A.S.; Pedrosa, F.O.; Souza, E.M.; Monteiro, R.A.; Chubatsu, L.S. [Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR (Brazil)

2015-07-10

NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein can still activate nif genes transcription; however, ammonium regulation is lost. In this work, we generated eight constructs resulting in point mutations in H. seropedicae NifA and analyzed their effect on nifH transcription in Escherichia coli and H. seropedicae. Mutations K22V, T160E, M161V, L172R, and A215D resulted in inactive proteins. Mutations Q216I and S220I produced partially active proteins with activity control similar to wild-type NifA. However, mutation G25E, located in the GAF domain, resulted in an active protein that did not require GlnK for activity and was partially sensitive to ammonium. This suggested that G25E may affect the negative interaction between the N-terminal GAF domain and the catalytic central domain under high ammonium concentrations, thus rendering the protein constitutively active, or that G25E could lead to a conformational change comparable with that when GlnK interacts with the GAF domain.

Progress towards polar-drive ignition for the NIF

Science.gov (United States)

McCrory, R. L.; Betti, R.; Boehly, T. R.; Casey, D. T.; Collins, T. J. B.; Craxton, R. S.; Delettrez, J. A.; Edgell, D. H.; Epstein, R.; Frenje, J. A.; Froula, D. H.; Gatu-Johnson, M.; Glebov, V. Yu.; Goncharov, V. N.; Harding, D. R.; Hohenberger, M.; Hu, S. X.; Igumenshchev, I. V.; Kessler, T. J.; Knauer, J. P.; Li, C. K.; Marozas, J. A.; Marshall, F. J.; McKenty, P. W.; Meyerhofer, D. D.; Michel, D. T.; Myatt, J. F.; Nilson, P. M.; Padalino, S. J.; Petrasso, R. D.; Radha, P. B.; Regan, S. P.; Sangster, T. C.; Séguin, F. H.; Seka, W.; Short, R. W.; Shvydky, A.; Skupsky, S.; Soures, J. M.; Stoeckl, C.; Theobald, W.; Yaakobi, B.; Zuegel, J. D.

2013-11-01

The University of Rochester's Laboratory for Laser Energetics (LLE) performs direct-drive inertial confinement fusion (ICF) research. LLE's Omega Laser Facility is used to study direct-drive ICF ignition concepts, developing an understanding of the underlying physics that feeds into the design of ignition targets for the National Ignition Facility (NIF). The baseline symmetric-illumination, direct-drive-ignition target design consists of a 1.5 MJ multiple-picket laser pulse that generates four shock waves (similar to the NIF baseline indirect-drive design) and is predicted to produce a one-dimensional (1D) gain of 48. LLE has developed the polar-drive (PD) illumination concept (for NIF beams in the x-ray-drive configuration) to allow the pursuit of direct-drive ignition without significant reconfiguration of the beam paths on the NIF. Some less-invasive changes in the NIF infrastructure will be required, including new phase plates, polarization rotators, and a PD-specific beam-smoothing front end. A suite of PD ignition designs with implosion velocities from 3.5 to 4.3 × 107 cm s-1 are predicted to have significant 2D gains (Collins et al 2012 Bull. Am. Phys. Soc. 57 155). Verification of the physics basis of these simulations is a major thrust of direct-drive implosion experiments on both OMEGA and the NIF. Many physics issues are being examined with symmetric beam irradiation on OMEGA, varying the implosion parameters over a wide region of design space. Cryogenic deuterium-tritium target experiments with symmetric irradiation have produced areal densities of ˜0.3 g cm-2, ion temperatures over 3 keV, and neutron yields in excess of 20% of the ‘clean’ 1D predicted value. The inferred Lawson criterion figure of merit (Betti R. et al 2010 Phys. Plasmas 17 058102) has increased from 1.7 atm s (IAEA 2010) to 2.6 atm s.

Tests and Calibration of the NIF Neutron Time of Flight Detectors

International Nuclear Information System (INIS)

Ali, Z.A.; Glebov, V.Yu.; Cruz, M.; Duffy, T.; Stoeckl, C.; Roberts, S.; Sangster, T.C.; Tommasini, R.; Throop, A; Moran, M.; Dauffy, L.; Horsefield, C.

2008-01-01

The National Ignition Facility (NIF) Neutron Time of Flight (NTOF) diagnostic will measure neutron yield and ion temperature in all NIF campaigns in DD, DT, and THD (D = deuterium, T = tritium, H = hydrogen) implosions. The NIF NTOF diagnostic is designed to measure neutron yield from 10 9 to 2 x 10 19 . The NTOF consists of several detectors of varying sensitivity located on the NIF at about 5 m and 20 m from the target. Production, testing, and calibration of the NIF NTOF detectors have begun at the Laboratory for Laser Energetics (LLE). Operational tests of the NTOF detectors were performed on several facilities including the OMEGA laser at LLE and the Titan laser at Lawrence Livermore National Laboratory (LLNL). Neutron calibrations were carried out on the OMEGA laser. Results of the NTOF detectors tests and calibration will be presented

Software quality assurance plan for the National Ignition Facility integrated computer control system

Energy Technology Data Exchange (ETDEWEB)

Woodruff, J.

1996-11-01

Quality achievement is the responsibility of the line organizations of the National Ignition Facility (NIF) Project. This Software Quality Assurance Plan (SQAP) applies to the activities of the Integrated Computer Control System (ICCS) organization and its subcontractors. The Plan describes the activities implemented by the ICCS section to achieve quality in the NIF Project`s controls software and implements the NIF Quality Assurance Program Plan (QAPP, NIF-95-499, L-15958-2) and the Department of Energy`s (DOE`s) Order 5700.6C. This SQAP governs the quality affecting activities associated with developing and deploying all control system software during the life cycle of the NIF Project.

The National Ignition Facility (NIF) and the issue of nonproliferation. Final study

International Nuclear Information System (INIS)

1995-01-01

NIF, the next step proposed by DOE in a progression of Inertial Confinement Fusion (ICF) facilities, is expected to reach the goal of ICF capsule ignition in the laboratory. This report is in response to a request of a Congressman that DOE resolve the question of whether NIF will aid or hinder U.S. nonproliferation efforts. Both technical and policy aspects are addressed, and public participation was part of the decision process. Since the technical proliferation concerns at NIF are manageable and can be made acceptable, and NIF can contribute positively to U.S. arms control and nonproliferation policy goals, it is concluded that NIF supports the nuclear nonproliferation objectives of the United States

The National Ignition Facility (NIF) and the issue of nonproliferation. Final study

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-12-19

NIF, the next step proposed by DOE in a progression of Inertial Confinement Fusion (ICF) facilities, is expected to reach the goal of ICF capsule ignition in the laboratory. This report is in response to a request of a Congressman that DOE resolve the question of whether NIF will aid or hinder U.S. nonproliferation efforts. Both technical and policy aspects are addressed, and public participation was part of the decision process. Since the technical proliferation concerns at NIF are manageable and can be made acceptable, and NIF can contribute positively to U.S. arms control and nonproliferation policy goals, it is concluded that NIF supports the nuclear nonproliferation objectives of the United States.

Inter-domain cross-talk controls the NifA protein activity of Herbaspirillum seropedicae.

Science.gov (United States)

Monteiro, R A; de Souza, E M; Wassem, R; Yates, M G; Pedrosa, F O; Chubatsu, L S

2001-11-09

Herbaspirillum seropedicae is an endophytic diazotroph, which colonizes sugar cane, wheat, rice and maize. The activity of NifA, a transcriptional activator of nif genes in H. seropedicae, is controlled by ammonium ions through a mechanism involving its N-terminal domain. Here we show that this domain interacts specifically in vitro with the N-truncated NifA protein, as revealed by protection against proteolysis, and this interaction caused an inhibitory effect on both the ATPase and DNA-binding activities of the N-truncated NifA protein. We suggest that the N-terminal domain inhibits NifA-dependent transcriptional activation by an inter-domain cross-talk between the catalytic domain of the NifA protein and its regulatory N-terminal domain in response to fixed nitrogen.

National Ignition Facility (NIF) FY2015 Facility Use Plan

Energy Technology Data Exchange (ETDEWEB)

Folta, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wisoff, Jeff [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2014-12-18

Major features of the FY2015 NIF Use Plan include: • Performing a record number of layered DT experiments with 28 planned compared with 15 in FY2014. Executing the first plutonium experiments on the NIF in support of the Science Campaigns. • Over 300 targets shots, a 57% increase compared to FY14. This is a stretch goal defined in the 120-Day Study document, and relies upon the success of many shot-rate improvement actions, as well as on the distribution of shot type selected by the users. While the Plan is consistent with this goal, the increased proportion of layered DT experiments described above reduces the margin against this goal. • Commissioning of initial ARC capability, which will support both SSP-HED and SSPICF programs. • Increase in days allocated to Discovery Science to a level that supports an ongoing program for academic use of NIF and an annual solicitation for new proposals. • Six Facility Maintenance and Reconfiguration (FM&R) periods totaling 30 days dedicated to major facility maintenance and modifications. • Utilization of the NIF Facility Advisory Schedule Committee (FASC) to provide stakeholder review and feedback on the NIF schedule. The Use Plan assumes a total FY2015 LLNL NIF Operations funding in MTE 10.7 of $229.465M and in MTE 10.3 of 47.0M. This Use Plan will be revised in the event of significant changes to the FY2015 funding or if NNSA provides FY2016 budget guidance significantly reduced compared to FY2015.

National Ignition Facility (NIF) Control Network Design and Analysis

International Nuclear Information System (INIS)

Bryant, R M; Carey, R W; Claybourn, R V; Pavel, G; Schaefer, W J

2001-01-01

The control network for the National Ignition Facility (NIF) is designed to meet the needs for common object request broker architecture (CORBA) inter-process communication, multicast video transport, device triggering, and general TCP/IP communication within the NIF facility. The network will interconnect approximately 650 systems, including the embedded controllers, front-end processors (FEPs), supervisory systems, and centralized servers involved in operation of the NIF. All systems are networked with Ethernet to serve the majority of communication needs, and asynchronous transfer mode (ATM) is used to transport multicast video and synchronization triggers. CORBA software infra-structure provides location-independent communication services over TCP/IP between the application processes in the 15 supervisory and 300 FEP systems. Video images sampled from 500 video cameras at a 10-Hz frame rate will be multicast using direct ATM Application Programming Interface (API) communication from video FEPs to any selected operator console. The Ethernet and ATM control networks are used to broadcast two types of device triggers for last-second functions in a large number of FEPs, thus eliminating the need for a separate infrastructure for these functions. Analysis, design, modeling, and testing of the NIF network has been performed to provide confidence that the network design will meet NIF control requirements

National Ignition Facility quality assurance plan for laser materials and optical technology

Energy Technology Data Exchange (ETDEWEB)

Wolfe, C.R.

1996-05-01

Quality achievement is the responsibility of the line organizations of the National Ignition Facility (NIF) Project. This subtier Quality Assurance Plan (QAP) applies to activities of the Laser Materials & Optical Technology (LM&OT) organization and its subcontractors. It responds to the NIF Quality Assurance Program Plan (QAPP, L-15958-2, NIF-95-499) and Department of Energy (DOE) Order 5700.6C. This Plan is organized according to 10 Quality Assurance (QA) criteria and subelements of a management system as outlined in the NIF QAPP. This Plan describes how those QA requirements are met. This Plan is authorized by the Associate Project Leader for the LM&OT organization, who has assigned responsibility to the Optics QA engineer to maintain this plan, with the assistance of the NIF QA organization. This Plan governs quality-affecting activities associated with: design; procurement; fabrication; testing and acceptance; handling and storage; and installation of NIF Project optical components into mounts and subassemblies.

National Ignition Facility quality assurance plan for laser materials and optical technology

International Nuclear Information System (INIS)

Wolfe, C.R.

1996-05-01

Quality achievement is the responsibility of the line organizations of the National Ignition Facility (NIF) Project. This subtier Quality Assurance Plan (QAP) applies to activities of the Laser Materials ampersand Optical Technology (LM ampersand OT) organization and its subcontractors. It responds to the NIF Quality Assurance Program Plan (QAPP, L-15958-2, NIF-95-499) and Department of Energy (DOE) Order 5700.6C. This Plan is organized according to 10 Quality Assurance (QA) criteria and subelements of a management system as outlined in the NIF QAPP. This Plan describes how those QA requirements are met. This Plan is authorized by the Associate Project Leader for the LM ampersand OT organization, who has assigned responsibility to the Optics QA engineer to maintain this plan, with the assistance of the NIF QA organization. This Plan governs quality-affecting activities associated with: design; procurement; fabrication; testing and acceptance; handling and storage; and installation of NIF Project optical components into mounts and subassemblies

IFE chamber technology testing program in NIF and chamber development test plan

International Nuclear Information System (INIS)

Abdou, M.A.

1995-01-01

Issues concerning chamber technology testing program in NIF involving: criteria for evaluation/prioritization of experiments, engineering scaling requirements for test article design and material selection and R and D plan prior to NIF testing were addressed in this paper. In order to maximize the benefits of testing program in NIF, the testing in NIF should provide the experimental data relevant to DEMO design choice or to DEMO design predictive capability by utilizing engineering scaling test article designs. Test plans were developed for 2 promising chamber design concepts. Early testing in non-fusion/non-ignition prior to testing in ignition facility serves a critical role in chamber R and D test plans in order to reduce the risks and costs of the more complex experiments in NIF

Control of autogenous activation of Herbaspirillum seropedicae nifA promoter by the IHF protein.

Science.gov (United States)

Wassem, Roseli; Pedrosa, Fábio O; Yates, Marshall G; Rego, Fabiane G M; Chubatsu, Leda S; Rigo, Liu U; Souza, Emanuel M

2002-07-02

Analysis of the expression of the Herbaspirillum seropedicae nifA promoter in Escherichia coli and Herbaspirillum seropedicae, showed that nifA expression is primarily dependent on NtrC but also required NifA for maximal expression under nitrogen-fixing conditions. Deletion of the IHF (integration host factor)-binding site produced a promoter with two-fold higher activity than the native promoter in the H. seropedicae wild-type strain but not in a nifA strain, indicating that IHF controls NifA auto-activation. IHF is apparently required to prevent overexpression of the NifA protein via auto-activation under nitrogen-fixing conditions in H. seropedicae.

Target diagnostic system for the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Leeper, R.J.; Chandler, G.A.; Cooper, G.W.; Derzon, M.S.

1996-01-01

A review of recent progress on the design of a diagnostic system proposed for ignition target experiments on the National Ignition Facility (NIF) will be presented. This diagnostic package contains an extensive suite of optical, x-ray, gamma-ray, and neutron diagnostics that enable measurements of the performance of both direct and indirect driven NIF targets. The philosophy used in designing all of the diagnostics in the set has emphasized redundant and independent measurement of fundamental physical quantities relevant to the operation of the NIF target. A unique feature of these diagnostics is that they are being designed to be capable of operating, in the high radiation, EMP, and debris backgrounds expected on the NIF facility. The diagnostic system proposed can be categorized into three broad areas: laser characterization, hohlraum characterization, and capsule performance diagnostics. The operating principles of a representative instrument from each class of diagnostic employed in this package will be summarized and illustrated with data obtained in recent prototype diagnostic tests

Inkludering av ungdom med minoritetsbakgrunn i NIF-organisert idrett

Directory of Open Access Journals (Sweden)

Mari Kristin Sisjord

2014-10-01

Full Text Available Norges Idrettsforbund og Olympiske og Paralympiske Komité (NIF har en uttalt målsetting om en åpen og inkluderende idrett. Hvordan kommer dette til uttrykk i den praktiske virksomheten? Denne artikkelen, som bygger på data fra en undersøkelse om likestilling og mangfold i den organiserte idretten (NIF, retter søkelyset mot arbeid med inklusjon av ungdom med minoritetsbakgrunn på ulike organisasjonsnivå i NIF: særforbund, idrettskretser og idrettslag. Datamaterialet er kvalitative intervju med representanter fra ulike organisasjonsnivå. Resultatene viser at NIFs overordnede politikk i varierende grad nedfelles i særforbundenes virksomhet, i idrettskretsene og i idrettslagene. Av særforbundene skiller Fotballforbundet og Klatreforbundet seg ut som aktive pådrivere i arbeidet med inkludering. Mange idrettslag oppfattet slike spørsmål som lite aktuelle i sitt rekrutteringsområde. Representanter for lag som hadde erfaring med inkludering og rekruttering av minoritetsungdom, tilkjennega varierte erfaringer og synspunkter.

Expression, purification, and functional analysis of the C-terminal domain of Herbaspirillum seropedicae NifA protein.

Science.gov (United States)

Monteiro, Rose A; Souza, Emanuel M; Geoffrey Yates, M; Steffens, M Berenice R; Pedrosa, Fábio O; Chubatsu, Leda S

2003-02-01

The Herbaspirillum seropedicae NifA protein is responsible for nif gene expression. The C-terminal domain of the H. seropedicae NifA protein, fused to a His-Tag sequence (His-Tag-C-terminal), was over-expressed and purified by metal-affinity chromatography to yield a highly purified and active protein. Band-shift assays showed that the NifA His-Tag-C-terminal bound specifically to the H. seropedicae nifB promoter region in vitro. In vivo analysis showed that this protein inhibited the Central + C-terminal domains of NifA protein from activating the nifH promoter of K. pneumoniae in Escherichia coli, indicating that the protein must be bound to the NifA-binding site (UAS site) at the nifH promoter region to activate transcription. Copyright 2002 Elsevier Science (USA)

Directed mutagenesis affects recombination in Azospirillum brasilense nif genes

Directory of Open Access Journals (Sweden)

C.P. Nunes

2000-12-01

Full Text Available In order to improve the gene transfer/mutagenesis system for Azospirillum brasilense, gene-cartridge mutagenesis was used to replace the nifD gene with the Tn5 kanamycin resistance gene. The construct was transferred to A. brasilense by electrotransformation. Of the 12 colonies isolated using the suicide plasmid pSUP202 as vector, only four did not show vector integration into the chromosome. Nevertheless, all 12 colonies were deficient in acetylene reduction, indicating an Nif- phenotype. Four Nif- mutants were analyzed by Southern blot, using six different probes spanning the nif and Km r genes and the plasmid vector. Apparently, several recombination events occurred in the mutant genomes, probably caused mainly by gene disruption owing to the mutagenesis technique used: resistance gene-cartridge mutagenesis combined with electrotransformation.Com o objetivo de melhorar os sistemas de transferência gênica e mutagênese para Azospirillum brasilense, a técnica de mutagênese através do uso de um gene marcador ("gene-cartridge mutagenesis" foi utilizada para substituir a região genômica de A. brasilense correspondente ao gene nifD por um segmento de DNA do transposon Tn5 contendo o gene que confere resistência ao antibiótico canamicina. A construção foi transferida para a linhagem de A. brasilense por eletrotransformação. Doze colônias transformantes foram isoladas com o plasmídeo suicida pSUP202 servindo como vetor. Dessas, somente quatro não possuíam o vetor integrado no cromossomo da bactéria. Independentemente da integração ou não do vetor, as 12 colônias foram deficientes na redução do gás acetileno, evidenciando o fenótipo Nif -. Quatro mutantes Nif - foram analisados através da técnica de Southern blot, utilizando-se seis diferentes fragmentos contendo genes nif, de resistência à canamicina e do vetor como sondas. Os resultados sugerem a ocorrência de eventos recombinacionais variados no genoma dos mutantes. A

Advances in inertial confinement fusion at the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Moses, Edward I.

2010-01-01

The 192-beam National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (LLNL) in Livermore, CA, is now operational and conducting experiments. NIF, the flagship facility of the U.S. Inertial Confinement Fusion (ICF) Program, will achieve high-energy-density conditions never previously obtained in the laboratory-temperatures over 100 million K, densities of 1000 g/cm 3 , and pressures exceeding 100 billion atmospheres. Such conditions exist naturally only in the interiors of the stars and during thermonuclear burn. Demonstration of ignition and thermonuclear burn in the laboratory is a major NIF goal. To date, the NIF laser has demonstrated all pulse shape, beam quality, energy, and other specifications required to meet the ignition challenge. On March 10, 2009, the NIF laser delivered 1.1 MJ of ultraviolet laser energy to target chamber center, approximately 30 times more energy than any previous facility. The ignition program at NIF is the National Ignition Campaign (NIC), a national collaboration for ignition experimentation with participation from General Atomics, LLNL, Los Alamos National Laboratory (LANL), Sandia National Laboratories (SNL), and the University of Rochester Laboratory for Laser Energetics (LLE). The achievement of ignition at NIF will demonstrate the scientific feasibility of ICF and focus worldwide attention on fusion as a viable energy option. A particular energy concept under investigation is the LIFE (Laser Inertial Fusion Energy) scheme. The LIFE engine is inherently safe, minimizes proliferation concerns associated with the nuclear fuel cycle, and can provide a sustainable carbon-free energy generation solution in the 21st century. This talk will describe NIF and its potential as a user facility and an experimental platform for high-energy-density science, NIC, and the LIFE approach for clean, sustainable energy.

Advances in Inertial Confinement Fusion at the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Moses, E.

2009-01-01

The 192-beam National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (LLNL) in Livermore, CA, is now operational and conducting experiments. NIF, the flagship facility of the U.S. Inertial Confinement Fusion (ICF) Program, will achieve high-energy-density conditions never previously obtained in the laboratory - temperatures over 100 million K, densities of 1,000 g/cm3, and pressures exceeding 100 billion atmospheres. Such conditions exist naturally only in the interiors of the stars and during thermonuclear burn. Demonstration of ignition and thermonuclear burn in the laboratory is a major NIF goal. To date, the NIF laser has demonstrated all pulse shape, beam quality, energy, and other specifications required to meet the ignition challenge. On March 10, 2009, the NIF laser delivered 1.1 MJ of ultraviolet laser energy to target chamber center, approximately 30 times more energy than any previous facility. The ignition program at NIF is the National Ignition Campaign (NIC), a national collaboration for ignition experimentation with participation from General Atomics, LLNL, Los Alamos National Laboratory (LANL), Sandia National Laboratories (SNL), and the University of Rochester Laboratory for Laser Energetics (LLE). The achievement of ignition at NIF will demonstrate the scientific feasibility of ICF and focus worldwide attention on fusion as a viable energy option. A particular energy concept under investigation is the LIFE (Laser Inertial Fusion Energy) scheme. The LIFE engine is inherently safe, minimizes proliferation concerns associated with the nuclear fuel cycle, and can provide a sustainable carbon-free energy generation solution in the 21st century. This talk will describe NIF and its potential as a user facility and an experimental platform for high-energy-density science, NIC, and the LIFE approach for clean, sustainable energy.

Progress towards polar-drive ignition for the NIF

International Nuclear Information System (INIS)

McCrory, R.L.; Betti, R.; Boehly, T.R.; Collins, T.J.B.; Craxton, R.S.; Delettrez, J.A.; Edgell, D.H.; Epstein, R.; Froula, D.H.; Glebov, V.Yu.; Goncharov, V.N.; Harding, D.R.; Hohenberger, M.; Hu, S.X.; Igumenshchev, I.V.; Kessler, T.J.; Knauer, J.P.; Casey, D.T.; Frenje, J.A.; Gatu-Johnson, M.

2013-01-01

The University of Rochester's Laboratory for Laser Energetics (LLE) performs direct-drive inertial confinement fusion (ICF) research. LLE's Omega Laser Facility is used to study direct-drive ICF ignition concepts, developing an understanding of the underlying physics that feeds into the design of ignition targets for the National Ignition Facility (NIF). The baseline symmetric-illumination, direct-drive–ignition target design consists of a 1.5 MJ multiple-picket laser pulse that generates four shock waves (similar to the NIF baseline indirect-drive design) and is predicted to produce a one-dimensional (1D) gain of 48. LLE has developed the polar-drive (PD) illumination concept (for NIF beams in the x-ray–drive configuration) to allow the pursuit of direct-drive ignition without significant reconfiguration of the beam paths on the NIF. Some less-invasive changes in the NIF infrastructure will be required, including new phase plates, polarization rotators, and a PD-specific beam-smoothing front end. A suite of PD ignition designs with implosion velocities from 3.5 to 4.3 × 10 7 cm s −1 are predicted to have significant 2D gains (Collins et al 2012 Bull. Am. Phys. Soc. 57 155). Verification of the physics basis of these simulations is a major thrust of direct-drive implosion experiments on both OMEGA and the NIF. Many physics issues are being examined with symmetric beam irradiation on OMEGA, varying the implosion parameters over a wide region of design space. Cryogenic deuterium–tritium target experiments with symmetric irradiation have produced areal densities of ∼0.3 g cm −2 , ion temperatures over 3 keV, and neutron yields in excess of 20% of the ‘clean’ 1D predicted value. The inferred Lawson criterion figure of merit (Betti R. et al 2010 Phys. Plasmas 17 058102) has increased from 1.7 atm s (IAEA 2010) to 2.6 atm s. (paper)

NifH- Harboring Bacterial Community Composition Across an Alaskan Permafrost Thaw Gradient

Directory of Open Access Journals (Sweden)

Christopher Ryan Penton

2016-11-01

Full Text Available Since nitrogen (N is often limiting in permafrost soils, we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal soil. Samples from minimally, moderately and extensively thawed sites were taken to a depth of 79 cm to encompass zones above and below the depth of the water table. NifH reads were translated with frameshift correction and 112,476 sequences were clustered at 5% amino acid dissimilarity resulting in 1,631 OTUs. Sample depth in relation to water table depth was correlated to differences in the NifH sequence classes. NifH sequences most closely related to group I nifH-harboring Alpha- and Beta Proteobacteria were in higher abundance above water table depth while those related to group III nifH-harboring Delta and Gamma Proteobacteria were more abundant below. The most dominant below water table depth NifH sequences, comprising 1/3 of the total, were distantly related to Verrucomicrobia-Opitutaceae. Overall, these results suggest that permafrost thaw alters the class-level composition of N2-fixing communities in the thawed soil layers and that this distinction corresponds to the depth of the water table. These nifH data were also compared to nifH sequences obtained from a study at an Alaskan taiga site, and to those of other geographically distant, non-permafrost sites. The two Alaska sites were differentiated largely by changes in relative abundances of the same OTUs, whereas the non-Alaska sites were differentiated by the lack of many Alaskan OTUs, and the presence of unique halophilic, sulfate- and iron-reducing taxa in the Alaska sites.

Song decrystallization in adult zebra finches does not require the song nucleus NIf.

Science.gov (United States)

Roy, Arani; Mooney, Richard

2009-08-01

In adult male zebra finches, transecting the vocal nerve causes previously stable (i.e., crystallized) song to slowly degrade, presumably because of the resulting distortion in auditory feedback. How and where distorted feedback interacts with song motor networks to induce this process of song decrystallization remains unknown. The song premotor nucleus HVC is a potential site where auditory feedback signals could interact with song motor commands. Although the forebrain nucleus interface of the nidopallium (NIf) appears to be the primary auditory input to HVC, NIf lesions made in adult zebra finches do not trigger song decrystallization. One possibility is that NIf lesions do not interfere with song maintenance, but do compromise the adult zebra finch's ability to express renewed vocal plasticity in response to feedback perturbations. To test this idea, we bilaterally lesioned NIf and then transected the vocal nerve in adult male zebra finches. We found that bilateral NIf lesions did not prevent nerve section-induced song decrystallization. To test the extent to which the NIf lesions disrupted auditory processing in the song system, we made in vivo extracellular recordings in HVC and a downstream anterior forebrain pathway (AFP) in NIf-lesioned birds. We found strong and selective auditory responses to the playback of the birds' own song persisted in HVC and the AFP following NIf lesions. These findings suggest that auditory inputs to the song system other than NIf, such as the caudal mesopallium, could act as a source of auditory feedback signals to the song motor network.

Software quality assurance plan for the National Ignition Facility integrated computer control system

International Nuclear Information System (INIS)

Woodruff, J.

1996-11-01

Quality achievement is the responsibility of the line organizations of the National Ignition Facility (NIF) Project. This Software Quality Assurance Plan (SQAP) applies to the activities of the Integrated Computer Control System (ICCS) organization and its subcontractors. The Plan describes the activities implemented by the ICCS section to achieve quality in the NIF Project's controls software and implements the NIF Quality Assurance Program Plan (QAPP, NIF-95-499, L-15958-2) and the Department of Energy's (DOE's) Order 5700.6C. This SQAP governs the quality affecting activities associated with developing and deploying all control system software during the life cycle of the NIF Project

Science on high-energy lasers: From today to the NIF

International Nuclear Information System (INIS)

Lee, R.W.; Petrasso, R.; Falcone, R.W.

1995-01-01

This document presents both a concise definition of the current capabilities of high energy lasers and a description of capabilities of the NIF (National Ignition Facility). Five scientific areas are discussed (Astrophysics, Hydrodynamics, Material Properties, Plasma Physics, Radiation Sources, and Radiative Properties). In these five areas we project a picture of the future based on investigations that are being carried on today. Even with this very conservative approach we find that the development of new higher energy lasers will make many extremely exciting areas accessible to us

User Interface Framework for the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Fisher, J M; Bowers, G A; Carey, R W; Daveler, S A; Herndon Ford, K B; Ho, J C; Lagin, L J; Lambert, C J; Mauvais, J; Stout, E A; West, S L

2007-01-01

A user interface (UI) framework supports the development of user interfaces to operate the National Ignition Facility (NIF) using the Integrated Computer Control System (ICCS). [1] This framework simplifies UI development and ensures consistency for NIF operators. A comprehensive, layered collection of UIs in ICCS provides interaction with system-level processes, shot automation, and subsystem-specific devices. All user interfaces are written in Java, employing CORBA to interact with other ICCS components. ICCS developers use these frameworks to compose two major types of user interfaces: broadviews and control panels. Broadviews provide a visual representation of the NIF beamlines through interactive schematic drawings. Control panels provide status and control at a device level. The UI framework includes a suite of display components to standardize user interaction through data entry behaviors, common connection and threading mechanisms, and a common appearance. With these components, ICCS developers can more efficiently address usability issues in the facility when needed. The ICCS UI framework helps developers create consistent and easy-to-understand user interfaces for NIF operators

National Ignition Facility system design requirements NIF integrated computer controls SDR004

International Nuclear Information System (INIS)

Bliss, E.

1996-01-01

This System Design Requirement document establishes the performance, design, development, and test requirements for the NIF Integrated Computer Control System. The Integrated Computer Control System (ICCS) is covered in NIF WBS element 1.5. This document responds directly to the requirements detailed in the NIF Functional Requirements/Primary Criteria, and is supported by subsystem design requirements documents for each major ICCS Subsystem

National Ignition Facility project acquisition plan

International Nuclear Information System (INIS)

Callaghan, R.W.

1996-04-01

The purpose of this National Ignition Facility Acquisition Plan is to describe the overall procurement strategy planned for the National Ignition Facility (NIF) Project. The scope of the plan describes the procurement activities and acquisition strategy for the following phases of the NIF Project, each of which receives either plant and capital equipment (PACE) or other project cost (OPC) funds: Title 1 and 2 design and Title 3 engineering (PACE); Optics manufacturing facilitization and pilot production (OPC); Convention facility construction (PACE); Procurement, installation, and acceptance testing of equipment (PACE); and Start-up (OPC). Activities that are part of the base Inertial Confinement Fusion (ICF) Program are not included in this plan. The University of California (UC), operating Lawrence Livermore National Laboratory (LLNL) and Los Alamos National Laboratory, and Lockheed-Martin, which operates Sandia National Laboratory (SNL) and the University of Rochester Laboratory for Laser Energetics (UR-LLE), will conduct the acquisition of needed products and services in support of their assigned responsibilities within the NIF Project structure in accordance with their prime contracts with the Department of Energy (DOE). LLNL, designated as the lead Laboratory, will have responsibility for all procurements required for construction, installation, activation, and startup of the NIF

Iron depletion affects nitrogenase activity and expression of nifH and nifA genes in Herbaspirillum seropedicae.

Science.gov (United States)

Rosconi, Federico; Souza, Emanuel M; Pedrosa, Fabio O; Platero, Raúl A; González, Cecilia; González, Marcela; Batista, Silvia; Gill, Paul R; Fabiano, Elena R

2006-05-01

Herbaspirillum seropedicae Z67 is a nitrogen-fixing bacterium able to colonize the rhizosphere and the interior of several plants. As iron is a key element for nitrogen fixation, we examined the response of this microorganism to iron deficiency under nitrogen fixing conditions. We identified a H. seropedicae exbD gene that was induced in response to iron limitation and is involved in iron homeostasis. We found that an exbD mutant grown in iron-chelated medium is unable to fix nitrogen. Moreover, we provide evidence that expression of the nifH and nifA genes is iron dependent in a H. seropedicae genetic background.

Orchestrating Shots for the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Mathisen, D G; Bettenhausen, R C; Beeler, R G; Bowers, G A; Carey, R W; Casavant, D D; Cline, B D; Demaret, R D; Domyancic, D M; Elko, S D; Fisher, J M; Krammen, J E; Lagin, L J; Ludwigsen, A P; Patterson, R W; Sanchez, R J; Stout, E A

2005-01-01

The National Ignition Facility (NIF), currently under construction at the Lawrence Livermore National Laboratory, is a stadium-sized facility containing a 192-beam, 1.8 Megajoule, 500-Terawatt, ultra-violet laser system together with a 10-meter diameter target chamber with room for nearly 100 experimental diagnostics. When completed, NIF will be the world's largest and most energetic laser experimental system, providing an international center to study inertial confinement fusion and physics of matter at extreme densities and pressures. The NIF is operated by the Integrated Computer Control System (ICCS), which is a layered architecture of over 700 lower-level front-end processors attached to nearly 60,000 control points and coordinated by higher-level supervisory subsystems in the main control room. A shot automation framework has been developed and deployed during the past year to orchestrate and automate shots performed at the NIF using the ICCS. The Shot Automation framework is designed to automate 4-8 hour shot sequences, that includes deriving shot goals from an experiment definition, set up of the laser and diagnostics, automatic alignment of laser beams, and a countdown to charge and fire the lasers. These sequences consist of set of preparatory verification shots, leading to amplified system shots followed by post-shot analysis and archiving. The framework provides for a flexible, model-based work-flow execution, driven by scripted automation called macro steps. The shot director software is the orchestrating component of a very flexible automation layer which allows us to define, coordinate and reuse simpler automation sequences. This software provides a restricted set of shot life cycle state transitions to 26 collaboration supervisors that automate 8-laser beams (bundle) and a common set of shared resources. Each collaboration supervisor commands approximately 10 subsystem shot supervisors that perform automated control and status verification

NIF diagnostic damage and design issues

International Nuclear Information System (INIS)

Landen, N.

1999-01-01

The NIF target environment is evaluated with respect to target and diagnostic debris and with respect to instrument survivability in the presence of target debris and radiation. Quantitative estimates are arrived at by extrapolating from Nova and Omega experience using simple scaling arguments. Specifically, we evaluate the closest distance of approach of various components of DIM-based diagnostics such as target mounted pinhole arrays, open detectors, filters, x-ray optics, and spectrometers. We also include constraints on achieving adequate signal-to-noise on x-ray diagnostics. Four of the most important conclusions are as follows: (1) The required full NIF stand-off distance for heavily filtered detectors (e.g. multi-keV x-ray and particle detectors) as determined by concerns of diagnostic debris and diagnostic survivability to debris and radiation is no more than 100 cm. (2) Target mounted pinhole arrays and slits mounted a few cm from chamber center at NIF will survive long enough to record data and should be an acceptable source of shrapnel debris. (3) DIM-based instrument stand-off distances are compatible with achieving the same photon statistics (or better with ongoing improvements in detector resolution and noise) than available with current Nova and Omega SIM- or TIM-based instrumentation. Section II reviews target and diagnostic debris with respect to final optics. Section III reviews debris and radiation with respect to all diagnostic components. The following laser scaling between Nova/Omega and NIF is used throughout unless otherwise specified: laser energy E = 100x, drive duration τ = 6x and hence for a given laser intensity or hohlraum temperature, target size r approximately √(E/τ) = 4x. The 100x increase in E accounts for the fact that many Nova shots were performed with only 20 kJ and all LLNL Omega shots were performed with only 15 kJ

NIF ICCS Test Controller for Automated and Manual Testing

International Nuclear Information System (INIS)

Zielinski, J S

2007-01-01

The National Ignition Facility (NIF) Integrated Computer Control System (ICCS) is a large (1.5 MSLOC), hierarchical, distributed system that controls all aspects of the NIF laser [1]. The ICCS team delivers software updates to the NIF facility throughout the year to support shot operations and commissioning activities. In 2006, there were 48 releases of ICCS: 29 full releases, 19 patches. To ensure the quality of each delivery, thousands of manual and automated tests are performed using the ICCS Test Controller test infrastructure. The TestController system provides test inventory management, test planning, automated test execution and manual test logging, release testing summaries and test results search, all through a web browser interface. Automated tests include command line based frameworks server tests and Graphical User Interface (GUI) based Java tests. Manual tests are presented as a checklist-style web form to be completed by the tester. The results of all tests, automated and manual, are kept in a common repository that provides data to dynamic status reports. As part of the 3-stage ICCS release testing strategy, the TestController system helps plan, evaluate and track the readiness of each release to the NIF facility

Management Prestart Review Phase 1 for the NIF Optics Assembly Building (OAB)

International Nuclear Information System (INIS)

Dragoo, V

2000-01-01

A Management Prestart Review (MPR) for the National Ignition Facility (NIF) Optics Assembly Building (OAB) was conducted from June, 2000, through October, 2000. This review was performed to determine readiness of the facility and management to transfer the facility from the construction to the commissioning and operations phase. This review process provides assurance that the appropriate line management is in place to effect the turnover. Completion and acceptance of this report constitutes a turnover of facility and equipment operational responsibility from the Beampath Infrastructure System Construction organization to the Assembly Installation and Refurbishment Operations (assembly equipment installation/activation and mechanical cleaning operations) and the Beampath Infrastructure System (BIS) Commissioning and Operations Organizations (conventional facility operations). The OAB MPR provides to the NIF Project Manager an independent, systematic assessment of: (1) Readiness of line management for the turnover to take place, (2) Completeness of the equipment and facility installation of the OAB, (3) Readiness of personnel to operate within the facility, and (4) Implementation and efficacy of key management control processes and procedures. The MPR process assures that the technical, cost, and schedule risk associated with the installation/activation of OAB special equipment, mechanical cleaning, and conventional facility operations within the OAB are evaluated and are acceptable. Specifically, the scope of the review addresses technical and operational attributes of the equipment and facility systems that have been determined to have significant project risk. This report implements the LLNL requirement that MPRs shall be conducted before all new facilities are brought into operation, Lawrence Livermore National Laboratory (LLNL) ES and H Manual (M-010), Section 2.2.5, Pre-Start Reviews. The MPR process is an essential part of the ISM work authorization and

NIF-type iron-sulfur cluster assembly system is duplicated and distributed in the mitochondria and cytosol of Mastigamoeba balamuthi.

Science.gov (United States)

Nývltová, Eva; Šuták, Robert; Harant, Karel; Šedinová, Miroslava; Hrdy, Ivan; Paces, Jan; Vlček, Čestmír; Tachezy, Jan

2013-04-30

In most eukaryotes, the mitochondrion is the main organelle for the formation of iron-sulfur (FeS) clusters. This function is mediated through the iron-sulfur cluster assembly machinery, which was inherited from the α-proteobacterial ancestor of mitochondria. In Archamoebae, including pathogenic Entamoeba histolytica and free-living Mastigamoeba balamuthi, the complex iron-sulfur cluster machinery has been replaced by an ε-proteobacterial nitrogen fixation (NIF) system consisting of two components: NifS (cysteine desulfurase) and NifU (scaffold protein). However, the cellular localization of the NIF system and the involvement of mitochondria in archamoebal FeS assembly are controversial. Here, we show that the genes for both NIF components are duplicated within the M. balamuthi genome. One paralog of each protein contains an amino-terminal extension that targets proteins to mitochondria (NifS-M and NifU-M), and the second paralog lacks a targeting signal, thereby reflecting the cytosolic form of the NIF machinery (NifS-C and NifU-C). The dual localization of the NIF system corresponds to the presence of FeS proteins in both cellular compartments, including detectable hydrogenase activity in Mastigamoeba cytosol and mitochondria. In contrast, E. histolytica possesses only single genes encoding NifS and NifU, respectively, and there is no evidence for the presence of the NIF machinery in its reduced mitochondria. Thus, M. balamuthi is unique among eukaryotes in that its FeS cluster formation is mediated through two most likely independent NIF machineries present in two cellular compartments.

FY17 NIF Performance Quad Campaign: laser performance results and conclusions

Energy Technology Data Exchange (ETDEWEB)

Di Nicola, J. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Mennerat, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Widmayer, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-10-09

The FY17 NIF Performance Quad Campaign exercised a single quad of NIF (Q45T) at elevated energy to assess the impact of recent improvements to the infrared (1ω) and ultraviolet (3ω) section of the laser on integrated performance.

Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7

Directory of Open Access Journals (Sweden)

Potrich D.P.

2001-01-01

Full Text Available A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In this region three nif operons have been identified: nifHDKorf1Y, nifENXorf3orf5fdxAnifQ and orf2nifUSVorf4. The operons containing nifENX and nifUSV genes are separated from the structural nifHDKorf1Y operon by about 5 kb and 10 kb, respectively. The present study shows the sequence analysis of the 6045-bp DNA region containing the nifENX genes. The deduced amino acid sequences from the open reading frames were compared to the nif gene products of other diazotrophic bacteria and indicate the presence of seven ORFs, all reading in the same direction as that of the nifHDKorf1Y operon. Consensus sigma54 and NifA-binding sites are present only in the promoter region upstream of the nifE gene. This promoter is activated by NifA protein and is approximately two-times less active than the nifH promoter, as indicated by the ß-galactosidase assays. This result suggests the differential expression of the nif genes and their respective products in Azospirillum.

A new synthetic approach to the science of complexity: the MISSION project at NIFS

International Nuclear Information System (INIS)

Tetsuya Sato

1999-01-01

The present day stage of computer simulation has entered into the third phase. The age of computer simulation in plasma physics dawned in the late 1950's when Oscar Buneman and John Dawson developed the sheet particle model. In 1960's and the early part of 1970's, one devoted oneself to refine the particle model and to develop, more practically, fluid magnetic hydrodynamic models so that the feasibility of the computer simulation methodology could be tested. This age can be called 'dawning of computer simulation'. The so-called supercomputer appeared in the late 1970's and the computer simulation entered the second phase where individual nonlinear phenomena have become possible to be attacked. At present when a supercomputer with the ability of higher than 100 GFlops speed and lager than 10GBytes common memory is available, almost any individual nonlinear phenomenon, whatever it may look complex, can be solved. This age may well be called 'the age of nonlinear solver'. However, as far as the authors are satisfied with using a supercomputer for simply solving an individual nonlinear problem, the computer simulation plays only a passive role in science and would never cause a catastrophic transition to it. Then, the modern science of the 20th century based on reductionism would continue in the coming 21st century, thus the 21st century's science would stay boring and tedious. It must be the computer simulation that can refresh this boring state. At NIFS an extensive effort has been made to establish a new paradigm of science in the 21st century by developing a new synthetic methodology of computer simulation, which the authors call the MISSION Project. The authors present this MISSION Project and propose a working hypothesis of the science of complexity in this talk

Interaction of an IHF-like protein with the Rhizobium etli nifA promoter.

Science.gov (United States)

Benhassine, Traki; Fauvart, Maarten; Vanderleyden, Jos; Michiels, Jan

2007-06-01

The nifA gene fulfills an essential role in the regulation of nitrogen fixation genes in Rhizobium etli. Transcription analysis of the nifA gene, assessed using promoter deletions, indicated an oxygen-independent expression, threefold higher during symbiosis as compared with free-living conditions. Electrophoretic mobility shift assays using those nifA promoter deletion fragments, which were actively transcribed, demonstrated the specific interaction with R. etli cellular protein(s) resulting in the formation of two DNA-protein complexes. An interacting protein was purified by liquid chromatography on Heparin Sepharose and Mono S columns. The purified 12 kDa R. etli protein cross-reacted with antibodies directed against Escherichia coli integration host factor (IHF). Furthermore, purified E. coli IHF was able to specifically bind to the R. etli nifA promoter region. These results point to an as yet undisclosed function of IHF in the regulation of R. etli nifA expression.

NIF special equipment construction health and safety plan

Energy Technology Data Exchange (ETDEWEB)

Sawicki, R.H.

1997-07-28

The purpose of this plan is to identify how the construction and deployment activities of the National Ignition Facility (NIF) Special Equipment (SE) will be safely executed. This plan includes an identification of (1) the safety-related responsibilities of the SE people and their interaction with other organizations involved; (2) safety related requirements, policies, and documentation; (3) a list of the potential hazards unique to SE systems and the mechanisms that will be implemented to control them to acceptable levels; (4) a summary of Environmental Safety and Health (ES&H) training requirements; and (5) requirements of contractor safety plans that will be developed and used by all SE contractors participating in site activities. This plan is a subsidiary document to the NIF Construction Safety Program (CSP) and is intended to compliment the requirements stated therein with additional details specific to the safety needs of the SE construction-related activities. If a conflict arises between these two documents, the CSP will supersede. It is important to note that this plan does not list all of the potential hazards and their controls because the design and safety analysis process is still ongoing. Additional safety issues win be addressed in the Final Safety Analysis Report, Operational Safety Procedures (OSPs), and other plans and procedures as described in Section 3.0 of this plan.

NIF special equipment construction health and safety plan

International Nuclear Information System (INIS)

Sawicki, R.H.

1997-01-01

The purpose of this plan is to identify how the construction and deployment activities of the National Ignition Facility (NIF) Special Equipment (SE) will be safely executed. This plan includes an identification of (1) the safety-related responsibilities of the SE people and their interaction with other organizations involved; (2) safety related requirements, policies, and documentation; (3) a list of the potential hazards unique to SE systems and the mechanisms that will be implemented to control them to acceptable levels; (4) a summary of Environmental Safety and Health (ES ampersand H) training requirements; and (5) requirements of contractor safety plans that will be developed and used by all SE contractors participating in site activities. This plan is a subsidiary document to the NIF Construction Safety Program (CSP) and is intended to compliment the requirements stated therein with additional details specific to the safety needs of the SE construction-related activities. If a conflict arises between these two documents, the CSP will supersede. It is important to note that this plan does not list all of the potential hazards and their controls because the design and safety analysis process is still ongoing. Additional safety issues win be addressed in the Final Safety Analysis Report, Operational Safety Procedures (OSPs), and other plans and procedures as described in Section 3.0 of this plan

Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.

OpenAIRE

MacNeil, D; Brill, W J

1980-01-01

Four Nif+ revertants from strains with polar insertions in nifL, were insensitive to ammonium and amino acid repression of nitrogenase synthesis. These strains have mutations located in or near the nifL region. The derepressed phenotype was dominant in a merodiploid containing a nif+ plasmid. These nif regulatory mutations also suppressed the Nif- phenotype of Gln- strains. Thus, regulation by fixed nitrogen (possible via glutamine synthetase) occurs on the nifLA operon but not on the other s...

NIF Target Assembly Metrology Methodology and Results

Energy Technology Data Exchange (ETDEWEB)

Alger, E. T. [General Atomics, San Diego, CA (United States); Kroll, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dzenitis, E. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Montesanti, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hughes, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Swisher, M. [IAP, Livermore, CA (United States); Taylor, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Segraves, K. [IAP, Livermore, CA (United States); Lord, D. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Reynolds, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Castro, C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Edwards, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2011-01-01

During our inertial confinement fusion (ICF) experiments at the National Ignition Facility (NIF) we require cryogenic targets at the 1-cm scale to be fabricated, assembled, and metrologized to micron-level tolerances. During assembly of these ICF targets, there are physical dimensmetrology is completed using optical coordinate measurement machines that provide repeatable measurements with micron precision, while also allowing in-process data collection for absolute accuracy in assembly. To date, 51 targets have been assembled and metrologized, and 34 targets have been successfully fielded on NIF relying on these metrology data. In the near future, ignition experiments on NIF will require tighter tolerances and more demanding target assembly and metrology capability. Metrology methods, calculations, and uncertainty estimates will be discussed. Target diagnostic port alignment, target position, and capsule location results will be reviewed for the 2009 Energetics Campaign. The information is presented via control charts showing the effect of process improvements that were made during target production. Certain parameters, including capsule position, met the 2009 campaign specifications but will have much tighter requirements in the future. Finally, in order to meet these new requirements assembly process changes and metrology capability upgrades will be necessary.

Automated optics inspection analysis for NIF

International Nuclear Information System (INIS)

Kegelmeyer, Laura M.; Clark, Raelyn; Leach, Richard R.; McGuigan, David; Kamm, Victoria Miller; Potter, Daniel; Salmon, J. Thad; Senecal, Joshua; Conder, Alan; Nostrand, Mike; Whitman, Pamela K.

2012-01-01

The National Ignition Facility (NIF) is a high-energy laser facility comprised of 192 beamlines that house thousands of optics. These optics guide, amplify and tightly focus light onto a tiny target for fusion ignition research and high energy density physics experiments. The condition of these optics is key to the economic, efficient and maximally energetic performance of the laser. Our goal, and novel achievement, is to find on the optics any imperfections while they are tens of microns in size, track them through time to see if they grow and if so, remove the optic and repair the single site so the entire optic can then be re-installed for further use on the laser. This paper gives an overview of the image analysis used for detecting, measuring, and tracking sites of interest on an optic while it is installed on the beamline via in situ inspection and after it has been removed for maintenance. In this way, the condition of each optic is monitored throughout the optic's lifetime. This overview paper will summarize key algorithms and technical developments for custom image analysis and processing and highlight recent improvements. (Associated papers will include more details on these issues.) We will also discuss the use of OI Analysis for daily operation of the NIF laser and its extension to inspection of NIF targets.

Automated optics inspection analysis for NIF

Energy Technology Data Exchange (ETDEWEB)

Kegelmeyer, Laura M., E-mail: kegelmeyer1@llnl.gov [Lawrence Livermore National Laboratory, Livermore, CA (United States); Clark, Raelyn; Leach, Richard R.; McGuigan, David; Kamm, Victoria Miller; Potter, Daniel; Salmon, J. Thad; Senecal, Joshua; Conder, Alan; Nostrand, Mike; Whitman, Pamela K. [Lawrence Livermore National Laboratory, Livermore, CA (United States)

2012-12-15

The National Ignition Facility (NIF) is a high-energy laser facility comprised of 192 beamlines that house thousands of optics. These optics guide, amplify and tightly focus light onto a tiny target for fusion ignition research and high energy density physics experiments. The condition of these optics is key to the economic, efficient and maximally energetic performance of the laser. Our goal, and novel achievement, is to find on the optics any imperfections while they are tens of microns in size, track them through time to see if they grow and if so, remove the optic and repair the single site so the entire optic can then be re-installed for further use on the laser. This paper gives an overview of the image analysis used for detecting, measuring, and tracking sites of interest on an optic while it is installed on the beamline via in situ inspection and after it has been removed for maintenance. In this way, the condition of each optic is monitored throughout the optic's lifetime. This overview paper will summarize key algorithms and technical developments for custom image analysis and processing and highlight recent improvements. (Associated papers will include more details on these issues.) We will also discuss the use of OI Analysis for daily operation of the NIF laser and its extension to inspection of NIF targets.

NIF Ignition Target 3D Point Design

Energy Technology Data Exchange (ETDEWEB)

Jones, O; Marinak, M; Milovich, J; Callahan, D

2008-11-05

We have developed an input file for running 3D NIF hohlraums that is optimized such that it can be run in 1-2 days on parallel computers. We have incorporated increasing levels of automation into the 3D input file: (1) Configuration controlled input files; (2) Common file for 2D and 3D, different types of capsules (symcap, etc.); and (3) Can obtain target dimensions, laser pulse, and diagnostics settings automatically from NIF Campaign Management Tool. Using 3D Hydra calculations to investigate different problems: (1) Intrinsic 3D asymmetry; (2) Tolerance to nonideal 3D effects (e.g. laser power balance, pointing errors); and (3) Synthetic diagnostics.

NIF: Impacts of chemical accidents and comparison of chemical/radiological accident approaches

International Nuclear Information System (INIS)

Lazaro, M.A.; Policastro, A.J.; Rhodes, M.

1996-01-01

The US Department of Energy (DOE) proposes to construct and operate the National Ignition Facility (NIF). The goals of the NIF are to (1) achieve fusion ignition in the laboratory for the first time by using inertial confinement fusion (ICF) technology based on an advanced-design neodymium glass solid-state laser, and (2) conduct high-energy-density experiments in support of national security and civilian applications. The primary focus of this paper is worker-public health and safety issues associated with postulated chemical accidents during the operation of NIF. The key findings from the accident analysis will be presented. Although NIF chemical accidents will be emphasized, the important differences between chemical and radiological accident analysis approaches and the metrics for reporting results will be highlighted. These differences are common EIS facility and transportation accident assessments

Diversity and expression of nitrogenase genes (nifH) from ectomycorrhizas of Corsican pine (Pinus nigra).

Science.gov (United States)

Izumi, Hironari; Anderson, Ian C; Alexander, Ian J; Killham, Ken; Moore, Edward R B

2006-12-01

The diversity of bacterial nitrogenase genes (nifH) and their mRNA transcription in ectomycorrhizas of Corsican pine (Pinus nigra) were examined. DNA and RNA were extracted from surface-sterilized and non-sterilized Corsican pine roots colonized by the ectomycorrhizal (ECM) fungi, Suillus variegatus and Tomentellopsis submollis. DNA-derived nifH polymerase chain reaction (PCR) products were obtained from all samples, but only a few reverse transcription PCRs for nifH mRNA were successful, suggesting that nitrogenase genes were not always transcribed. Several different nifH sequences were detected and the bacteria actively transcribing nifH were different from those whose genes were detected through DNA-based PCR. Putative nitrogenase amino acid sequences revealed that more than half of the nifH products were derived from methylotrophic bacteria, such as Methylocella spp. The next most frequent sequence types were similar to those from Burkholderia.

Quality Control, Testing, and Deployment Results in the NIF ICCS

International Nuclear Information System (INIS)

Woodruff, J P; Casavant, D; Cline, B D; Gorvad, M R

2001-01-01

The strategy used to develop the NIF Integrated Computer Control System (ICCS) calls for incremental cycles of construction and formal test to deliver a total of 1 million lines of code. Each incremental release takes four to six months to implement specific functionality and culminates when offline tests conducted in the ICCS Integration and Test Facility verify functional, performance, and interface requirements. Tests are then repeated on line to confirm integrated operation in dedicated laser laboratories or ultimately in the NIF. Test incidents along with other change requests are recorded and tracked to closure by the software change control board (SCCB). Annual independent audits advise management on software process improvements. Extensive experience has been gained by integrating controls in the prototype laser preamplifier laboratory. The control system installed in the preamplifier lab contains five of the ten planned supervisory subsystems and seven of sixteen planned front-end processors (FEPs). Beam alignment, timing, diagnosis and laser pulse amplification up to 20 joules was tested through an automated series of shots. Other laboratories have provided integrated testing of six additional FEPs. Process measurements including earned-value, product size, and defect densities provide software project controls and generate confidence that the control system will be successfully deployed

Interaction of GlnK with the GAF domain of Herbaspirillum seropedicae NifA mediates NH₄⁺-regulation.

Science.gov (United States)

Oliveira, Marco A S; Aquino, Bruno; Bonatto, Ana Claudia; Huergo, Luciano F; Chubatsu, Leda S; Pedrosa, Fábio O; Souza, Emanuel M; Dixon, Ray; Monteiro, Rose A

2012-04-01

Nitrogen fixation in Herbaspirillum seropedicae is transcriptionally regulated by NifA, a σ(54) transcriptional activator with three structural domains: an N-terminal GAF domain, a catalytic AAA+ domain and a C-terminal DNA-binding domain. NifA is only active in H. seropedicae when cultures are grown in the absence of fixed nitrogen and at low oxygen tensions. There is evidence that the inactivation of NifA in response to fixed nitrogen is mediated by the regulatory GAF domain. However, the mechanism of NifA repression by the GAF domain, as well as the transduction of nitrogen status to NifA, is not understood. In order to study the regulation of NifA activity by fixed nitrogen independently of oxygen regulation, we constructed a chimeric protein containing the GAF domain of H. seropedicae NifA fused to the AAA+ and C-terminal domains of Azotobacter vinelandii NifA. This chimeric protein (NifAQ1) lacks the cysteine motif found in oxygen sensitive NifA proteins and is not oxygen responsive in vivo. Our results demonstrate that NifAQ1 responds to fixed nitrogen and requires GlnK protein for activity, a behavior similar to H. seropedicae NifA. In addition, protein footprinting analysis indicates that this response probably involves a protein-protein contact between the GAF domain and the GlnK protein. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Regulation of nif expression in Methanococcus maripaludis: roles of the euryarchaeal repressor NrpR, 2-oxoglutarate, and two operators.

Science.gov (United States)

Lie, Thomas J; Wood, Gwendolyn E; Leigh, John A

2005-02-18

The methanogenic archaean Methanococcus maripaludis can use ammonia, alanine, or dinitrogen as a nitrogen source for growth. The euryarchaeal nitrogen repressor NrpR controls the expression of the nif (nitrogen fixation) operon, resulting in full repression with ammonia, intermediate repression with alanine, and derepression with dinitrogen. NrpR binds to two tandem operators in the nif promoter region, nifOR(1) and nifOR(2). Here we have undertaken both in vivo and in vitro approaches to study the way in which NrpR, nifOR(1), nifOR(2), and the effector 2-oxoglutarate (2OG) combine to regulate nif expression, leading to a comprehensive understanding of this archaeal regulatory system. We show that NrpR binds as a dimer to nifOR(1) and cooperatively as two dimers to both operators. Cooperative binding occurs only with both operators present. nifOR(1) has stronger binding and by itself can mediate the repression of nif transcription during growth on ammonia, unlike the weakly binding nifOR(2). However, nifOR(2) in combination with nifOR(1) is critical for intermediate repression during growth on alanine. Accordingly, NrpR binds to both operators together with higher affinity than to nifOR(1) alone. NrpR responds directly to 2OG, which weakens its binding to the operators. Hence, 2OG is an intracellular indicator of nitrogen deficiency and acts as an inducer of nif transcription via NrpR. This model is upheld by the recent finding (J. A. Dodsworth and J. A. Leigh, submitted for publication) in our laboratory that 2OG levels in M. maripaludis vary with growth on different nitrogen sources.

Advances in target design and fabrication for experiments on NIF

Directory of Open Access Journals (Sweden)

Obrey K.

2013-11-01

Full Text Available The ability to build target platforms for National Ignition Facility (NIF is a key feature in LANL's (Los Alamos National Laboratory Target Fabrication Program. We recently built and manufactured the first LANL targets to be fielded on NIF in March 2011. Experiments on NIF require precision component manufacturing and accurate knowledge of the materials used in the targets. The characterization of foams and aerogels, the Be ignition capsule, and machining unique components are of main material focus. One important characterization metric the physics' have determined is that the knowledge of density gradients in foams is important. We are making strides in not only locating these density gradients in aerogels and foams as a result of how they are manufactured and machined but also quantifying the density within the foam using 3D confocal micro x-ray fluorescence (μXRF imaging and 3D x-ray computed tomography (CT imaging. In addition, collaborative efforts between General Atomics (GA and LANL in the characterization of the NIF Ignition beryllium capsule have shown that the copper in the capsule migrates radially from the capsule center.

NifI inhibits nitrogenase by competing with Fe protein for binding to the MoFe protein

International Nuclear Information System (INIS)

Dodsworth, Jeremy A.; Leigh, John A.

2007-01-01

Reduction of substrate by nitrogenase requires direct electron transfer from the Fe protein to the MoFe protein. Inhibition of nitrogenase activity in Methanococcus maripaludis occurs when the regulatory protein NifI 1,2 binds the MoFe protein. This inhibition is relieved by 2-oxoglutarate. Here we present evidence that NifI 1,2 binding prevents association of the two nitrogenase components. Increasing amounts of Fe protein competed with NifI 1,2 , decreasing its inhibitory effect. NifI 1,2 prevented the co-purification of MoFe protein with a mutant form of the Fe protein that forms a stable complex with the MoFe protein, and NifI 1,2 was unable to bind to an AlF 4 - -stabilized Fe protein:MoFe protein complex. NifI 1,2 inhibited ATP- and MoFe protein-dependent oxidation of the Fe protein, and 2OG relieved this inhibition. These results support a model where NifI 1,2 competes with the Fe protein for binding to MoFe protein and prevents electron transfer

The role of the NIF in the development of inertial fusion energy

International Nuclear Information System (INIS)

Logan, B.G.

1995-01-01

Recent decisions by DOE to proceed with the National Ignition Facility (NIF) and the first half of the Induction Systems Linac Experiments (ILSE) can provide the scientific basis for inertial fusion ignition and high-repetition heavy-ion driver physics, respectively. Both are critical to Inertial Fusion Energy (IFE). A conceptual design has been completed for a 1.8-MJ, 500-TW, 0.35-microm-solid-state laser system, the NIF. The NIF will demonstrate inertial fusion ignition and gain for national security applications, and for IFE development. It will support science applications using high-power lasers. The demonstration of inertial fusion ignition and gain, along with the parallel demonstration of the feasibility of an efficient, high-repetition-rate driver, would provide the basis for a follow-on Engineering Test Facility (ETF) identified in the National Energy Policy Act of 1992. The ETF would provide an integrated testbed for the development and demonstration of the technologies needed for IFE power plants. In addition to target physics of ignition, the NIF will contribute important data on IFE target chamber issues, including neutron damage, activation, target debris clearing, operational experience in many areas prototypical to future IFE power plants, and an opportunity to provide tests of candidate low-cost IFE targets and injection systems. An overview of the NIF design and the target area environments relevant to conducting IFE experiments are described in Section 2. In providing this basic data for IFE, the NIF will provide confidence that an ETF can be successful in the integration of drivers, target chambers, and targets for IFE

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense

Directory of Open Access Journals (Sweden)

C.Y. Nishikawa

2012-02-01

Full Text Available Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL. A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH4Cl but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense

Energy Technology Data Exchange (ETDEWEB)

Nishikawa, C.Y.; Araújo, L.M.; Kadowaki, M.A.S.; Monteiro, R.A.; Steffens, M.B.R.; Pedrosa, F.O.; Souza, E.M.; Chubatsu, L.S. [Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR (Brazil)

2012-01-27

Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ{sup 54} factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH{sub 4}Cl) but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense.

Science.gov (United States)

Nishikawa, C Y; Araújo, L M; Kadowaki, M A S; Monteiro, R A; Steffens, M B R; Pedrosa, F O; Souza, E M; Chubatsu, L S

2012-02-01

Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ(54) co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH(4)Cl) but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense

International Nuclear Information System (INIS)

Nishikawa, C.Y.; Araújo, L.M.; Kadowaki, M.A.S.; Monteiro, R.A.; Steffens, M.B.R.; Pedrosa, F.O.; Souza, E.M.; Chubatsu, L.S.

2012-01-01

Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ 54 factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH 4 Cl) but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription

Hierarchical material models for fragmentation modeling in NIF-ALE-AMR

International Nuclear Information System (INIS)

Fisher, A C; Masters, N D; Koniges, A E; Anderson, R W; Gunney, B T N; Wang, P; Becker, R; Dixit, P; Benson, D J

2008-01-01

Fragmentation is a fundamental process that naturally spans micro to macroscopic scales. Recent advances in algorithms, computer simulations, and hardware enable us to connect the continuum to microstructural regimes in a real simulation through a heterogeneous multiscale mathematical model. We apply this model to the problem of predicting how targets in the NIF chamber dismantle, so that optics and diagnostics can be protected from damage. The mechanics of the initial material fracture depend on the microscopic grain structure. In order to effectively simulate the fragmentation, this process must be modeled at the subgrain level with computationally expensive crystal plasticity models. However, there are not enough computational resources to model the entire NIF target at this microscopic scale. In order to accomplish these calculations, a hierarchical material model (HMM) is being developed. The HMM will allow fine-scale modeling of the initial fragmentation using computationally expensive crystal plasticity, while the elements at the mesoscale can use polycrystal models, and the macroscopic elements use analytical flow stress models. The HMM framework is built upon an adaptive mesh refinement (AMR) capability. We present progress in implementing the HMM in the NIF-ALE-AMR code. Additionally, we present test simulations relevant to NIF targets

Hierarchical material models for fragmentation modeling in NIF-ALE-AMR

Energy Technology Data Exchange (ETDEWEB)

Fisher, A C; Masters, N D; Koniges, A E; Anderson, R W; Gunney, B T N; Wang, P; Becker, R [Lawrence Livermore National Laboratory, PO Box 808, Livermore, CA 94551 (United States); Dixit, P; Benson, D J [University of California San Diego, 9500 Gilman Dr., La Jolla. CA 92093 (United States)], E-mail: fisher47@llnl.gov

2008-05-15

Fragmentation is a fundamental process that naturally spans micro to macroscopic scales. Recent advances in algorithms, computer simulations, and hardware enable us to connect the continuum to microstructural regimes in a real simulation through a heterogeneous multiscale mathematical model. We apply this model to the problem of predicting how targets in the NIF chamber dismantle, so that optics and diagnostics can be protected from damage. The mechanics of the initial material fracture depend on the microscopic grain structure. In order to effectively simulate the fragmentation, this process must be modeled at the subgrain level with computationally expensive crystal plasticity models. However, there are not enough computational resources to model the entire NIF target at this microscopic scale. In order to accomplish these calculations, a hierarchical material model (HMM) is being developed. The HMM will allow fine-scale modeling of the initial fragmentation using computationally expensive crystal plasticity, while the elements at the mesoscale can use polycrystal models, and the macroscopic elements use analytical flow stress models. The HMM framework is built upon an adaptive mesh refinement (AMR) capability. We present progress in implementing the HMM in the NIF-ALE-AMR code. Additionally, we present test simulations relevant to NIF targets.

Optomechanical considerations for the VISAR diagnostic at the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Morris I. Kaufman, John R. Celeste, Brent C. Frogget, Tony L. Lee, Brian J. GacGowan, Robert M. Malone, Edmund W. Ng, Tom W. Tunnell, Phillip W. Watts

2006-01-01

The National Ignition Facility (NIF) requires optical diagnostics for measuring shock velocities in shock physics experiments. The velocity interferometer for any reflector measures shock velocities at a location remote to the NIF target chamber. Our team designed two systems, one for a polar port orientation, and the other to accommodate two equatorial ports. The polar-oriented design requires a 48-m optical relay to move the light from inside the target chamber to a separately housed measurement and laser illumination station. The currently operational equatorial design requires a much shorter relay of 21 m. Both designs posed significant optomechanical challenges due to the long optical path length, large quantity of optical elements, and stringent NIF requirements. System design had to tightly control the use of lubricants and materials, especially those inside the vacuum chamber; tolerate earthquakes and radiation; and consider numerous other tolerance, alignment, and steering adjustment issues. To ensure compliance with NIF performance requirements, we conducted a finite element analysis

NIF Double Shell outer/inner shell collision experiments

Science.gov (United States)

Merritt, E. C.; Loomis, E. N.; Wilson, D. C.; Cardenas, T.; Montgomery, D. S.; Daughton, W. S.; Dodd, E. S.; Desjardins, T.; Renner, D. B.; Palaniyappan, S.; Batha, S. H.; Khan, S. F.; Smalyuk, V.; Ping, Y.; Amendt, P.; Schoff, M.; Hoppe, M.

2017-10-01

Double shell capsules are a potential low convergence path to substantial alpha-heating and ignition on NIF, since they are predicted to ignite and burn at relatively low temperatures via volume ignition. Current LANL NIF double shell designs consist of a low-Z ablator, low-density foam cushion, and high-Z inner shell with liquid DT fill. Central to the Double Shell concept is kinetic energy transfer from the outer to inner shell via collision. The collision determines maximum energy available for compression and implosion shape of the fuel. We present results of a NIF shape-transfer study: two experiments comparing shape and trajectory of the outer and inner shells at post-collision times. An outer-shell-only target shot measured the no-impact shell conditions, while an `imaging' double shell shot measured shell conditions with impact. The `imaging' target uses a low-Z inner shell and is designed to perform in similar collision physics space to a high-Z double shell but can be radiographed at 16keV, near the viable 2DConA BL energy limit. Work conducted under the auspices of the U.S. DOE by LANL under contract DE-AC52-06NA25396.

The National Ignition Facility (NIF) Diagnostic Set at the Completion of the National Ignition Campaign (NIC) September 2013

Energy Technology Data Exchange (ETDEWEB)

Kilkenny, J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bell, P. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bradley, D. K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bleuel, D. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Caggiano, J. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dewald, E. L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hsing, W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kalantar, H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kauffman, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Moody, J. D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Schneider, M. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Shaughnessy, D. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Shelton, R. T. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Yeamans, C. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Batha, S. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Grim, G. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Herrmann, H. W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Merrill, F. E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Leeper, R. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sangster, T. C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Edgell, D. H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glebov, V. Y. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Regan, S. P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Frenje, J. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gatu-Johnson, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Petrasso, R. D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Rindernecht, H. G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Zylstra, A. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Cooper, G. W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ruiz, C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-01-05

At the completion of the National Ignition Campaign NIF had about 36 different types of diagnostics. These were based on several decades of development on Nova and OMEGA and involved the whole US ICF community. A plan for a limited of NIF Diagnostics was documented by the Joint Central Diagnostic Team in the NIF Conceptual Design Report in 1994. These diagnostics and many more were installed diagnostics by two decades later. We give a short description of each of the 36 different types of NIC diagnostics grouped by the function of the diagnostics, namely target drive, target response and target assembly, stagnation and burn. A comparison of NIF diagnostics with the Nova diagnostics shows that the NIF diagnostic capability is broadly equivalent to that of Nova’s in 1999. NIF diagnostics have a much greater degree of automation and rigor than Nova’s and the NIF diagnostic suite incorporates some scientific innovation compared to Nova and OMEGA namely one much higher speed x-ray imager. Directions for future NIF diagnostics are discussed.

Nitrogenase activity of Herbaspirillum seropedicae grown under low iron levels requires the products of nifXorf1 genes.

Science.gov (United States)

Klassen, Giseli; de Oliveira Pedrosa, Fábio; de Souza, Emanuel M; Yates, M Geoffrey; Rigo, Liu Un

2003-07-29

Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the contiguous gene orf1 are essential for maximum nitrogen fixation under iron limitation and are probably involved in synthesis of nitrogenase iron or iron-molybdenum clusters.

Characterization of a 65 kDa NIF in the nuclear matrix of the monocot Allium cepa that interacts with nuclear spectrin-like proteins.

Science.gov (United States)

Pérez-Munive, Clara; Blumenthal, Sonal S D; de la Espina, Susana Moreno Díaz

2012-01-01

Plant cells have a well organized nucleus and nuclear matrix, but lack orthologues of the main structural components of the metazoan nuclear matrix. Although data is limited, most plant nuclear structural proteins are coiled-coil proteins, such as the NIFs (nuclear intermediate filaments) in Pisum sativum that cross-react with anti-intermediate filament and anti-lamin antibodies, form filaments 6-12 nm in diameter in vitro, and may play the role of lamins. We have investigated the conservation and features of NIFs in a monocot species, Allium cepa, and compared them with onion lamin-like proteins. Polyclonal antisera against the pea 65 kDa NIF were used in 1D and 2D Western blots, ICM (imunofluorescence confocal microscopy) and IEM (immunoelectron microscopy). Their presence in the nuclear matrix was analysed by differential extraction of nuclei, and their association with structural spectrin-like proteins by co-immunoprecipitation and co-localization in ICM. NIF is a conserved structural component of the nucleus and its matrix in monocots with Mr and pI values similar to those of pea 65 kDa NIF, which localized to the nuclear envelope, perichromatin domains and foci, and to the nuclear matrix, interacting directly with structural nuclear spectrin-like proteins. Its similarities with some of the proteins described as onion lamin-like proteins suggest that they are highly related or perhaps the same proteins.

Studies on the roles of GlnK and GlnB in regulating Klebsiella pneumoniae NifL-dependent nitrogen control.

NARCIS (Netherlands)

Arcondeguy, T.; van Heeswijk, W.C.; Merrick, M.

1999-01-01

In Klebsiella pneumoniae, nitrogen fixation (nif) genes are regulated in response to fixed nitrogen and oxygen. The activity of the nif-specific transcriptional activator NifA is modulated by NifL, which mediates both oxygen and nitrogen control. The signal transduction protein GlnK is required to

Capsule physics comparison of different ablators for NIF implosion designs

Science.gov (United States)

Clark, Daniel; Kritcher, Andrea; Yi, Austin; Zylstra, Alex; Haan, Steven; Ralph, Joseph; Weber, Christopher

2017-10-01

Indirect drive implosion experiments on the Naitonal Ignition Facility (NIF) have now tested three different ablator materials: glow discharge polymer (GDP) plastic, high density carbon (HDC), and beryllium. How do these different ablator choices compare in current and future implosion experiments on NIF? What are the relative advantages and disadvantages of each? This talk compares these different ablator options in capsule-only simulations of current NIF experiments and proposed future designs. The simulations compare the impact of the capsule fill tube, support tent, and interface surface roughness for each case, as well as all perturbations in combination. According to the simulations, each ablator is impacted by the various perturbation sources differently, and each material poses unique challenges in the pursuit of ignition. This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Organization of BSc and MSc projects in project families

DEFF Research Database (Denmark)

Ottosen, Lisbeth M.; Goltermann, Per; Jensen, Pernille Erland

2014-01-01

This work reports organization of student thesis projects in project families, with the benefit to both teaching and learning. The project organization went from student projects broadly distributed on topics related to different research issues and individual supervision to project families with...

The NIF: An international high energy density science and inertial fusion user facility

Directory of Open Access Journals (Sweden)

Moses E.I.

2013-11-01

Full Text Available The National Ignition Facility (NIF, a 1.8-MJ/500-TW Nd:Glass laser facility designed to study inertial confinement fusion (ICF and high-energy-density science (HEDS, is operational at Lawrence Livermore National Laboratory (LLNL. A primary goal of NIF is to create the conditions necessary to demonstrate laboratory-scale thermonuclear ignition and burn. NIF experiments in support of indirect-drive ignition began late in FY2009 as part of the National Ignition Campaign (NIC, an international effort to achieve fusion ignition in the laboratory. To date, all of the capabilities to conduct implosion experiments are in place with the goal of demonstrating ignition and developing a predictable fusion experimental platform in 2012. The results from experiments completed are encouraging for the near-term achievement of ignition. Capsule implosion experiments at energies up to 1.6 MJ have demonstrated laser energetics, radiation temperatures, and symmetry control that scale to ignition conditions. Of particular importance is the demonstration of peak hohlraum temperatures near 300 eV with overall backscatter less than 15%. Important national security and basic science experiments have also been conducted on NIF. Successful demonstration of ignition and net energy gain on NIF will be a major step towards demonstrating the feasibility of laser-driven Inertial Fusion Energy (IFE. This paper will describe the results achieved so far on the path toward ignition, the beginning of fundamental science experiments and the plans to transition NIF to an international user facility providing access to HEDS and fusion energy researchers around the world.

The NIF: An international high energy density science and inertial fusion user facility

Science.gov (United States)

Moses, E. I.; Storm, E.

2013-11-01

The National Ignition Facility (NIF), a 1.8-MJ/500-TW Nd:Glass laser facility designed to study inertial confinement fusion (ICF) and high-energy-density science (HEDS), is operational at Lawrence Livermore National Laboratory (LLNL). A primary goal of NIF is to create the conditions necessary to demonstrate laboratory-scale thermonuclear ignition and burn. NIF experiments in support of indirect-drive ignition began late in FY2009 as part of the National Ignition Campaign (NIC), an international effort to achieve fusion ignition in the laboratory. To date, all of the capabilities to conduct implosion experiments are in place with the goal of demonstrating ignition and developing a predictable fusion experimental platform in 2012. The results from experiments completed are encouraging for the near-term achievement of ignition. Capsule implosion experiments at energies up to 1.6 MJ have demonstrated laser energetics, radiation temperatures, and symmetry control that scale to ignition conditions. Of particular importance is the demonstration of peak hohlraum temperatures near 300 eV with overall backscatter less than 15%. Important national security and basic science experiments have also been conducted on NIF. Successful demonstration of ignition and net energy gain on NIF will be a major step towards demonstrating the feasibility of laser-driven Inertial Fusion Energy (IFE). This paper will describe the results achieved so far on the path toward ignition, the beginning of fundamental science experiments and the plans to transition NIF to an international user facility providing access to HEDS and fusion energy researchers around the world.

Spectroscopic and functional characterization of iron-bound forms of Azotobacter vinelandii (Nif)IscA.

Science.gov (United States)

Mapolelo, Daphne T; Zhang, Bo; Naik, Sunil G; Huynh, Boi Hanh; Johnson, Michael K

2012-10-16

The ability of Azotobacter vinelandii(Nif)IscA to bind Fe has been investigated to assess the role of Fe-bound forms in NIF-specific Fe-S cluster biogenesis. (Nif)IscA is shown to bind one Fe(III) or one Fe(II) per homodimer and the spectroscopic and redox properties of both the Fe(III)- and Fe(II)-bound forms have been characterized using the UV-visible absorption, circular dichroism, and variable-temperature magnetic circular dichroism, electron paramagnetic resonance, Mössbauer and resonance Raman spectroscopies. The results reveal a rhombic intermediate-spin (S = 3/2) Fe(III) center (E/D = 0.33, D = 3.5 ± 1.5 cm(-1)) that is most likely 5-coordinate with two or three cysteinate ligands and a rhombic high spin (S = 2) Fe(II) center (E/D = 0.28, D = 7.6 cm(-1)) with properties similar to reduced rubredoxins or rubredoxin variants with three cysteinate and one or two oxygenic ligands. Iron-bound (Nif)IscA undergoes reversible redox cycling between the Fe(III)/Fe(II) forms with a midpoint potential of +36 ± 15 mV at pH 7.8 (versus NHE). l-Cysteine is effective in mediating release of free Fe(II) from both the Fe(II)- and Fe(III)-bound forms of (Nif)IscA. Fe(III)-bound (Nif)IscA was also shown to be a competent iron source for in vitro NifS-mediated [2Fe-2S] cluster assembly on the N-terminal domain of NifU, but the reaction occurs via cysteine-mediated release of free Fe(II) rather than direct iron transfer. The proposed roles of A-type proteins in storing Fe under aerobic growth conditions and serving as iron donors for cluster assembly on U-type scaffold proteins or maturation of biological [4Fe-4S] centers are discussed in light of these results.

Enhanced hydrogen storage properties of MgH2 co-catalyzed with K2NiF6 and CNTs.

Science.gov (United States)

Sulaiman, N N; Ismail, M

2016-12-06

The composite of MgH 2 /K 2 NiF 6 /carbon nanotubes (CNTs) is prepared by ball milling, and its hydrogenation properties are studied for the first time. MgH 2 co-catalyzed with K 2 NiF 6 and CNTs exhibited an improvement in the onset dehydrogenation temperature and isothermal de/rehydrogenation kinetics compared with the MgH 2 -K 2 NiF 6 composite. The onset dehydrogenation temperature of MgH 2 doped with 10 wt% K 2 NiF 6 and 5 wt% CNTs is 245 °C, which demonstrated a reduction of 25 °C compared with the MgH 2 + 10 wt% K 2 NiF 6 composite. In terms of rehydrogenation kinetics, MgH 2 doped with 10 wt% K 2 NiF 6 and 5 wt% CNTs samples absorbed 3.4 wt% of hydrogen in 1 min at 320 °C, whereas the MgH 2 + 10 wt% K 2 NiF 6 sample absorbed 2.6 wt% of hydrogen under the same conditions. For dehydrogenation kinetics at 320 °C, the MgH 2 + 10 wt% K 2 NiF 6 + 5 wt% CNTs sample released 3.3 wt% hydrogen after 5 min of dehydrogenation. By contrast, MgH 2 doped with 10 wt% K 2 NiF 6 released 3.0 wt% hydrogen in the same time period. The apparent activation energy, E a , for the dehydrogenation of MgH 2 doped with 10 wt% K 2 NiF 6 reduced from 100.0 kJ mol -1 to 70.0 kJ mol -1 after MgH 2 was co-doped with 10 wt% K 2 NiF 6 and 5 wt% CNTs. Based on the experimental results, the hydrogen storage properties of the MgH 2 /K 2 NiF 6 /CNTs composite is enhanced because of the catalytic effects of the active species of KF, KH and Mg 2 Ni that are formed in situ during dehydrogenation, as well as the unique structure of CNTs.

Molybdenum x-ray absorption studies of the mutant Kp nifV of nitrogenase MO-FE protein

International Nuclear Information System (INIS)

Eidsness, M.K.; Smith, B.E.; Flood, A.C.; Garner, C.D.; Cramer, S.P.

1985-01-01

The nifV mutant nitrogenase enzyme of Klebsiella pheumoniae exhibits altered substrate reducing activity. This nitrogenase mutant cannot fix N 2 in vivo but can reduce C 2 H 2 to C 2 H 4 . The nifV mutant enzyme differs further from the wild-type enzyme by CO inhibition of its H 2 evolution activity, up to 80%. The NifV - phenotype (NifV - Kp1) has been shown to be associated with the iron-molybdenum cofactor (FeMoco) in the Mo Fe protein which is generally accepted as the site for substrate reduction. An X-Ray absorption study of the Mo site in this mutant may reveal a difference in its FeMoco structure. The authors report here a comparison of Mo X-Ray absorption data from the nitrogenase enzymes of the wild-type and NifV - strains in three different forms: (1) as isolated, (2) dye-oxidized, and (3) fixing enzyme systems. Mo edge structure of NifV - Kp1 and wild-type enzymes are nearly identical. Small shifts to higher energies are observed in the oxidized and fixing states. Mo EXAFS of NifV - Kp1 and wild-type in the ''as isolated'' state appear indistinguishable. Curve fitting results best describe the molybdenum in FeMoco as bound by 4-5 S atoms at 2.36 A ,3 Fe atoms at 2.69 A, and 0-2 O(N) atoms at 2.19 A. The spectral similarity of these results concerning the nifV mutant FeMoco structure is discussed

The National Ignition Facility (NIF) and High Energy Density Science Research at LLNL (Briefing Charts)

Science.gov (United States)

2013-06-21

The National Ignition Facility ( NIF ) and High Energy Density Science Research at LLNL Presentation to: IEEE Pulsed Power and Plasma Science...Conference C. J. Keane Director, NIF User Office June 21, 2013 1491978-1-4673-5168-3/13/$31.00 ©2013 IEEE Report Documentation Page Form ApprovedOMB No...4. TITLE AND SUBTITLE The National Ignition Facility ( NIF ) and High Energy Density Science Research at LLNL 5a. CONTRACT NUMBER 5b. GRANT

Report on the B-Fields at NIF Workshop Held at LLNL October 12-13, 2015

International Nuclear Information System (INIS)

Fournier, K. B.; Moody, J. D.

2015-01-01

A national ICF laboratory workshop on requirements for a magnetized target capability on NIF was held by NIF at LLNL on October 12 and 13, attended by experts from LLNL, SNL, LLE, LANL, GA, and NRL. Advocates for indirect drive (LLNL), magnetic (Z) drive (SNL), polar direct drive (LLE), and basic science needing applied B (many institutions) presented and discussed requirements for the magnetized target capabilities they would like to see. 30T capability was most frequently requested. A phased operation increasing the field in steps experimentally can be envisioned. The NIF management will take the inputs from the scientific community represented at the workshop and recommend pulse-powered magnet parameters for NIF that best meet the collective user requests. In parallel, LLNL will continue investigating magnets for future generations that might be powered by compact laser-B-field generators (Moody, Fujioka, Santos, Woolsey, Pollock). The NIF facility engineers will start to analyze compatibility of the recommended pulsed magnet parameters (size, field, rise time, materials) with NIF chamber constraints, diagnostic access, and final optics protection against debris in FY16. The objective of this assessment will be to develop a schedule for achieving an initial Bfield capability. Based on an initial assessment, room temperature magnetized gas capsules will be fielded on NIF first. Magnetized cryo-ice-layered targets will take longer (more compatibility issues). Magnetized wetted foam DT targets (Olson) may have somewhat fewer compatibility issues making them a more likely choice for the first cryo-ice-layered target fielded with applied Bz.

Antagonism of CD11b with neutrophil inhibitory factor (NIF) inhibits vascular lesions in diabetic retinopathy.

Science.gov (United States)

Veenstra, Alexander A; Tang, Jie; Kern, Timothy S

2013-01-01

Leukocytes and proteins that govern leukocyte adhesion to endothelial cells play a causal role in retinal abnormalities characteristic of the early stages of diabetic retinopathy, including diabetes-induced degeneration of retinal capillaries. Leukocyte integrin αmβ2 (CD11b/CD18, MAC1), a protein mediating adhesion, has been shown to mediate damage to endothelial cells by activated leukocytes in vitro. We hypothesized that Neutrophil Inhibitory Factor (NIF), a selective antagonist of integrin αmβ2, would inhibit the diabetes-induced degeneration of retinal capillaries by inhibiting the excessive interaction between leukocytes and retinal endothelial cells in diabetes. Wild type animals and transgenic animals expressing NIF were made diabetic with streptozotocin and assessed for diabetes-induced retinal vascular abnormalities and leukocyte activation. To assess if the leukocyte blocking therapy compromised the immune system, animals were challenged with bacteria. Retinal superoxide production, leukostasis and leukocyte superoxide production were increased in wild type mice diabetic for 10 weeks, as was the ability of leukocytes isolated from diabetic animals to kill retinal endothelial cells in vitro. Retinal capillary degeneration was significantly increased in wild type mice diabetic 40 weeks. In contrast, mice expressing NIF did not develop any of these abnormalities, with the exception that non-diabetic and diabetic mice expressing NIF generated greater amounts of superoxide than did similar mice not expressing NIF. Importantly, NIF did not significantly impair the ability of mice to clear an opportunistic bacterial challenge, suggesting that NIF did not compromise immune surveillance. We conclude that antagonism of CD11b (integrin αmβ2) by NIF is sufficient to inhibit early stages of diabetic retinopathy, while not compromising the basic immune response.

LANL C10.2 Projects in FY13

International Nuclear Information System (INIS)

Batha, Steven H.; Fincke, James R.; Schmitt, Mark J.

2012-01-01

LANL has two projects in C10.2: Defect-Induced Mix Experiment (DIME) (ongoing, several runs at Omega; NIF shots this summer); and Shock/Shear (tested at Omega for two years; NIF shots in second half of FY13). Each project is jointly funded by C10.2, other C10 MTEs, and Science Campaigns. DIME is investigating 4π and feature-induced mix in spherically convergent ICF implosions by using imaging of the mix layer. DIME prepared for NIF by demonstrating its PDD mix platform on Omega including imaging mid-Z doped layers and defects. DIME in FY13 will focus on PDD symmetry-dependent mix and moving burn into the mix region for validation of mix/burn models. Re-Shock and Shear are two laser-driven experiments designed to study the turbulent mixing of materials. In FY-2012 43 shear and re-shock experimental shots were executed on the OMEGA laser and a complete time history obtained for both. The FY-2013 goal is to transition the experiment to NIF where the larger scale will provide a longer time period for mix layer growth.

Producing KDP and DKDP crystals for the NIF laser

International Nuclear Information System (INIS)

Atherton, L. J.; Burnham, A. K.; Combs, R. C.; Couture, S. A.; De Yoreo, J. J.; Hawley-Fedder, R. A.; Montesant, R. C.; Robey, H. F.; Runkel, M.; Staggs, M.; Wegner, P. J.; Yan, M.; Zaitseva, N. P.

1999-01-01

The cost and physics requirements of the NIF have established two important roles for potassium dihydrogen phosphate (KDP) crystals. 1. To extract more laser energy per unit of flashlamp light and laser glass, the NIF has adopted a multipass architecture as shown in Figure 1. Light is injected in the transport spatial filter, first traverses the power amplifiers, and then is directed to main amplifiers, where it makes four passes before being redirected through the power amplifiers towards the target. To enable the multipass of the main amplifiers, a KDP-containing Pockels cell rotates the polarization of the beam to make it either transmit through or reflect off a polarizer held at Brewster's angle within the main laser cavity. If transmitted, the light reflects off a mirror and makes another pass through the cavity. If reflected, it proceeds through the power amplifier to the target. the original seed crystal as the pyramid faces grow. Unfortunately, this pyramidal growth is very slow, and it takes about two years to grow a crystal to NIF size. To provide more programmatic flexibility and reduce costs in the long run, we have developed an alternative technology commonly called rapid growth. Through a combination of higher temperatures and higher supersaturation of the growth solution, a NIF-size boule can be grown in 1 to 2 months from a small ''point'' seed. However, growing boules of adequate size is not sufficient. Care must be taken to prevent inclusions of growth solution and incorporation of atomically substituted 2. Implosions for ICF work far better at shorter wavelengths due to less generation of hot electrons, which preheat the fuel and make it harder to compress. Compromising between optic lifetime and implosion efficiency, both Nova and the NIF operate at a tripled frequency of the 1053-nm fundamental frequency of a neodymium glass laser. This tripling is accomplished by two crystals, one made of KDP and one made of deuterated KDP (DKDP). The first

NIF unconverted light and its influence on DANTE measurements.

Science.gov (United States)

Girard, Frederic; Suter, Larry; Landen, Otto; Munro, Dave; Regan, Sean; Kline, John

2009-06-01

NIF laser facility produces 1053 nm light and a fundamental requirement for NIF is to give up to 1.8 MJ of 351 nm light for target physics experiments. The 351 nm light is provided by frequency tripling the 1053 nm light in nonlinear crystals in the final optics assembly, just before the laser light enters the target chamber. Since this tripling process is not 100% efficient, unconverted light from the conversion process also enters the chamber. This unconverted light does not directly hit the target but it can strike target support structures at average intensities of few TW/cm2 where it can generate unwanted, background soft x-rays that are measured by the soft x-ray diagnostic DANTE installed on the NIF target chamber. This diagnostic quantifies the x-radiation intensity inside the hohlraum by measuring the x-ray flux coming from the target's laser entrance hole. Due to its centimeter wide field of view, it integrates x-ray emission from both the flux exiting a hohlraum laser entrance hole and from the target support structure irradiated by residual 1omega and 2omega unconverted light. This work gives quantitative evaluations of the unconverted light for the first time and the effects on DANTE measurements for the future NIF tuning experiment called "Shock timing." Emission spectra are significantly modified leading to an overestimation of radiative temperature during the foot of the laser pulse since background x-rays are predominant in first two DANTE channel measurements. Mitigations of these effects by coating silicon paddle with plastic, using a smaller collimator to reduce DANTE field of view or eliminating DANTE channels in the analysis have been investigated.

Target Diagnostics Supports NIF's Path to Ignition

International Nuclear Information System (INIS)

Shelton, R.

2011-01-01

The physics requirements derived from the National Ignition Facility (NIF) experimental campaigns are leading to a wide variety of target diagnostics. Software development for the control and analysis of these diagnostics is included in the NIF Integrated Computer Control System, Diagnostic Control System and Data Visualization. These projects implement the configuration, controls, data analysis and visual representation of most of these diagnostics. To date, over 40 target diagnostics have been developed to support NIF experiments. In 2011 diagnostics were developed or enhanced to measure Ignition performance in a high neutron yield environment. Performance is optimized around four key variables: Adiabat (a) which is the strength and timing of four shocks delivered to the target, Velocity (V) of the imploding target, Mix (M) is the uniformity of the burn, and the Shape (S) of the imploding Deuterium Tritium (DT) hot spot. The diagnostics used to measure each of these parameters is shown in figure 1. Adiabat is measured using the Velocity Interferometer System for Any Reflector (VISAR) diagnostic consisting of three streak cameras. To provide for more accurate adiabat measurements the VISAR streak cameras were enhanced in FY11 with a ten comb fiducial signal controller to allow for post shot correction of the streak camera sweep non-linearity. Mix is measured by the Neutron Time of Flight (NTOF) and Radiochemical Analysis of Gaseous Samples (RAGS) diagnostics. To accommodate high neutron yield shots, NTOF diagnostic controls are being modified to use Mach Zehnder interferometer signals to allow the digitizers to be moved from near the target chamber to the neutron shielded diagnostic mezzanine. In December 2011 the first phase of RAGS diagnostic commissioning will be completed. This diagnostic will analyze the tracers that are added to NIF target capsules that undergo nuclear reactions during the shot. These gases are collected and purified for nuclear counting by

Spectroscopic and functional characterization of iron-sulfur cluster-bound forms of Azotobacter vinelandii (Nif)IscA.

Science.gov (United States)

Mapolelo, Daphne T; Zhang, Bo; Naik, Sunil G; Huynh, Boi Hanh; Johnson, Michael K

2012-10-16

The mechanism of [4Fe-4S] cluster assembly on A-type Fe-S cluster assembly proteins, in general, and the specific role of (Nif)IscA in the maturation of nitrogen fixation proteins are currently unknown. To address these questions, in vitro spectroscopic studies (UV-visible absorption/CD, resonance Raman and Mössbauer) have been used to investigate the mechanism of [4Fe-4S] cluster assembly on Azotobacter vinelandii(Nif)IscA, and the ability of (Nif)IscA to accept clusters from NifU and to donate clusters to the apo form of the nitrogenase Fe-protein. The results show that (Nif)IscA can rapidly and reversibly cycle between forms containing one [2Fe-2S](2+) and one [4Fe-4S](2+) cluster per homodimer via DTT-induced two-electron reductive coupling of two [2Fe-2S](2+) clusters and O(2)-induced [4Fe-4S](2+) oxidative cleavage. This unique type of cluster interconversion in response to cellular redox status and oxygen levels is likely to be important for the specific role of A-type proteins in the maturation of [4Fe-4S] cluster-containing proteins under aerobic growth or oxidative stress conditions. Only the [4Fe-4S](2+)-(Nif)IscA was competent for rapid activation of apo-nitrogenase Fe protein under anaerobic conditions. Apo-(Nif)IscA was shown to accept clusters from [4Fe-4S] cluster-bound NifU via rapid intact cluster transfer, indicating a potential role as a cluster carrier for delivery of clusters assembled on NifU. Overall the results support the proposal that A-type proteins can function as carrier proteins for clusters assembled on U-type proteins and suggest that they are likely to supply [2Fe-2S] clusters rather than [4Fe-4S] for the maturation of [4Fe-4S] cluster-containing proteins under aerobic or oxidative stress growth conditions.

Abundance and genetic diversity of nifH gene sequences in anthropogenically affected Brazilian mangrove sediments.

Science.gov (United States)

Dias, Armando Cavalcante Franco; Pereira e Silva, Michele de Cassia; Cotta, Simone Raposo; Dini-Andreote, Francisco; Soares, Fábio Lino; Salles, Joana Falcão; Azevedo, João Lúcio; van Elsas, Jan Dirk; Andreote, Fernando Dini

2012-11-01

Although mangroves represent ecosystems of global importance, the genetic diversity and abundance of functional genes that are key to their functioning scarcely have been explored. Here, we present a survey based on the nifH gene across transects of sediments of two mangrove systems located along the coast line of São Paulo state (Brazil) which differed by degree of disturbance, i.e., an oil-spill-affected and an unaffected mangrove. The diazotrophic communities were assessed by denaturing gradient gel electrophoresis (DGGE), quantitative PCR (qPCR), and clone libraries. The nifH gene abundance was similar across the two mangrove sediment systems, as evidenced by qPCR. However, the nifH-based PCR-DGGE profiles revealed clear differences between the mangroves. Moreover, shifts in the nifH gene diversities were noted along the land-sea transect within the previously oiled mangrove. The nifH gene diversity depicted the presence of nitrogen-fixing bacteria affiliated with a wide range of taxa, encompassing members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and also a group of anaerobic sulfate-reducing bacteria. We also detected a unique mangrove-specific cluster of sequences denoted Mgv-nifH. Our results indicate that nitrogen-fixing bacterial guilds can be partially endemic to mangroves, and these communities are modulated by oil contamination, which has important implications for conservation strategies.

Abundance and Genetic Diversity of nifH Gene Sequences in Anthropogenically Affected Brazilian Mangrove Sediments

Science.gov (United States)

Dias, Armando Cavalcante Franco; Pereira e Silva, Michele de Cassia; Cotta, Simone Raposo; Dini-Andreote, Francisco; Soares, Fábio Lino; Salles, Joana Falcão; Azevedo, João Lúcio; van Elsas, Jan Dirk

2012-01-01

Although mangroves represent ecosystems of global importance, the genetic diversity and abundance of functional genes that are key to their functioning scarcely have been explored. Here, we present a survey based on the nifH gene across transects of sediments of two mangrove systems located along the coast line of São Paulo state (Brazil) which differed by degree of disturbance, i.e., an oil-spill-affected and an unaffected mangrove. The diazotrophic communities were assessed by denaturing gradient gel electrophoresis (DGGE), quantitative PCR (qPCR), and clone libraries. The nifH gene abundance was similar across the two mangrove sediment systems, as evidenced by qPCR. However, the nifH-based PCR-DGGE profiles revealed clear differences between the mangroves. Moreover, shifts in the nifH gene diversities were noted along the land-sea transect within the previously oiled mangrove. The nifH gene diversity depicted the presence of nitrogen-fixing bacteria affiliated with a wide range of taxa, encompassing members of the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and also a group of anaerobic sulfate-reducing bacteria. We also detected a unique mangrove-specific cluster of sequences denoted Mgv-nifH. Our results indicate that nitrogen-fixing bacterial guilds can be partially endemic to mangroves, and these communities are modulated by oil contamination, which has important implications for conservation strategies. PMID:22941088

AN UPDATE ON THE STATUS OF THE NIF POWER CONDITIONING SYSTEM

International Nuclear Information System (INIS)

Arnold, P A; Hulsey, S; Ullery, G T; Petersen, D E; Pendleton, D L; Ollis, C W; Newton, M A; Harwell, T; Cordoza, D; Hadovski, L

2007-01-01

The National Ignition Facility (NIF) Power Conditioning System provides the pulsed excitation required to drive flashlamps in the laser's optical amplifiers. Modular in design, each of the 192 Main Energy Storage Modules (MESMs) stores up to 2.2 MJ of electrical energy in its capacitor bank before delivering the energy to 20 pairs of flashlamps in a 400 (micro)s pulse (10% power points). The peak current of each MESM discharge is 0.5 MA. Production, installation, commissioning and operation of the NIF Power Conditioning continue to progress rapidly, with the goals of completing accelerated production and commissioning by early 2008, while maintaining an aggressive operation schedule. To date, more than 97% of the required modules have been assembled, shipped and installed in the facility, representing more that 380 MJ of stored energy available for driving NIF flashlamps. The MESMs have displayed outstanding reliability during daily, multiple-shift operations

Control of Herbaspirillum seropedicae NifA Activity by Ammonium Ions and Oxygen

Science.gov (United States)

Souza, E. M.; Pedrosa, F. O.; Drummond, M.; Rigo, L. U.; Yates, M. G.

1999-01-01

The activity of a truncated form of Herbaspirillum seropedicae NifA in different genetic backgrounds showed that its regulatory domain is involved in nitrogen control but not in O2 sensitivity or Fe dependence. The model for nitrogen control involving PII could thus apply to the proteobacteria at large. NifA may have a role in controlling ADP-ribosylation of nitrogenase in Azospirillum brasilense. PMID:9882688

Antagonism of CD11b with neutrophil inhibitory factor (NIF inhibits vascular lesions in diabetic retinopathy.

Directory of Open Access Journals (Sweden)

Alexander A Veenstra

Full Text Available Leukocytes and proteins that govern leukocyte adhesion to endothelial cells play a causal role in retinal abnormalities characteristic of the early stages of diabetic retinopathy, including diabetes-induced degeneration of retinal capillaries. Leukocyte integrin αmβ2 (CD11b/CD18, MAC1, a protein mediating adhesion, has been shown to mediate damage to endothelial cells by activated leukocytes in vitro. We hypothesized that Neutrophil Inhibitory Factor (NIF, a selective antagonist of integrin αmβ2, would inhibit the diabetes-induced degeneration of retinal capillaries by inhibiting the excessive interaction between leukocytes and retinal endothelial cells in diabetes. Wild type animals and transgenic animals expressing NIF were made diabetic with streptozotocin and assessed for diabetes-induced retinal vascular abnormalities and leukocyte activation. To assess if the leukocyte blocking therapy compromised the immune system, animals were challenged with bacteria. Retinal superoxide production, leukostasis and leukocyte superoxide production were increased in wild type mice diabetic for 10 weeks, as was the ability of leukocytes isolated from diabetic animals to kill retinal endothelial cells in vitro. Retinal capillary degeneration was significantly increased in wild type mice diabetic 40 weeks. In contrast, mice expressing NIF did not develop any of these abnormalities, with the exception that non-diabetic and diabetic mice expressing NIF generated greater amounts of superoxide than did similar mice not expressing NIF. Importantly, NIF did not significantly impair the ability of mice to clear an opportunistic bacterial challenge, suggesting that NIF did not compromise immune surveillance. We conclude that antagonism of CD11b (integrin αmβ2 by NIF is sufficient to inhibit early stages of diabetic retinopathy, while not compromising the basic immune response.

Organizations, projects and culture

NARCIS (Netherlands)

J. van Cleeff; Pieter van Nispen tot Pannerden

2016-01-01

Purpose: to explore and demonstrate the effects of organizational culture on projects, in particular project culture and project management style. Methodology/approach: descriptive and explorative; through students’ groups. Findings: the cultural relationship between organizations, their projects

Functional participation of a nifH-arsA2 chimeric fusion gene in arsenic reduction by Escherichia coli

International Nuclear Information System (INIS)

Lahiri, Surobhi; Pulakat, Lakshmi; Gavini, Nara

2008-01-01

The NifH (dimer) and ArsA proteins are structural homologs and share common motifs like nucleotide-binding domains, signal transduction domains and also possible similar metal center ligands. Given the similarity between two proteins, we investigated if the NifH protein from Azotobacter vinelandii could functionally substitute for the ArsA1 half of the ArsA protein of Escherichia coli. The chimeric NifH-ArsA2 protein was expressed and detected in the E. coli strain by Western blotting. Growth comparisons of E. coli strains containing plasmids encoding for complete ArsA, partial ArsA (ArsA2) or chimeric ArsA (NifH-ArsA2) in media with increasing sodium arsenite concentrations (0-5 mM) showed that the chimeric NifH-ArsA2 could substitute for the ArsA. This functional complementation demonstrated the strong conservation of essential domains that have been maintained in NifH and ArsA even after their divergence to perform varied functions

3(omega) Power Balance Procedure on the NIF

International Nuclear Information System (INIS)

Glenzer, S; Jones, O; Speck, D R; Munro, D; Lerche, R; Salmon, T; Bliss, E; Gates, A; Boyd, B; Auerbach, J; Williams, W; Saroyan, A; Kalantar, D; MacGowan, B; Zacharias, R; Hayman, C; Sacks, R

2001-01-01

This document defines the detailed NIF full system shot procedure to obtain 8% power balance as specified by the SDR002 3.2.1.04. Because the 48 quads of the NIF will be set up over a period of five years, obtaining power balance will naturally be accomplished in two steps. First, as each quad is brought online, the four laser beams within each quad will be tuned by setting the PABTS splitter ratios so that each beam will give the same laser power on target during low energy square pulse shots. During the quad activation period all of the technical tools and procedures will be developed that are needed for attaining full laser power balance. After the initial settings of the 48 PABTS, if no other tuning is done the overall NIF power balance is expected to be about <15%. In the second step, an iteration procedure with approximately 18 full laser system shots will be needed to obtain 8% power balance by tuning out the remaining systematic differences among the quads to an acceptable small difference of 2% rms (at 3ω). This rms difference is smaller than the expected variation of the injection energy or the amplifier gain, and is also of the same order as the laser energy diagnostic accuracy. Therefore, 8% power balance will require a number of precision measurements that will need accurate calibrations combined with a laser performance model that accounts and corrects for variations of the injection energy and the amplifier gain. This document is intended to specify the procedure and the flow-down of requirements from the system design requirement of 8% power balance. It is further intended to help guide the laser shot planning, the laser controls, and the laser performance operations model groups. It should provide input relevant to power balance tuning for the development of an operations model that includes post-shot analysis (as described in NIF-0046491), shot planning (as described in this memo), and pre-shot analysis

Alternative irradiation schemes for NIF and LMJ hohlraums

Science.gov (United States)

Bourgade, Jean-Luc; Bowen, Christopher; Gauthier, Pascal; Landen, Otto

2018-02-01

We explore two alternative irradiation schemes for the large (‘outer’) and small (‘inner’) angle beams that currently illuminate National Ignition Facility (NIF) and Laser Mégajoule cavities. In the first, while the outer laser beams enter through the usual end laser entrance holes (LEH), the inner beams enter through slots along the cavity axis wall, illuminating the back wall of the cavity. This avoids the current interaction of the inner laser beams with the gold wall bubbles generated by the outer beams, which leads to large time-dependent changes in drive symmetry. Another scheme potentially useful for NIF uses only the outer beams. The radiative losses through the slots or from the use of outer beams only are compensated by using a smaller cavity and LEH.

Role of the laboratory for laser energetics in the National Ignition Facility Project

International Nuclear Information System (INIS)

Soures, J.M.; Loucks, S.J.; McCrory, R.L.

1996-01-01

The National Ignition Facility (NIF) is a 192-beam, 1.8-MJ (ultraviolet) laser facility that is currently planned to start operating in 2002. The NIF mission is to provide data critical to this Nation's science-based stockpile stewardship (SBSS) program and to advance the understanding of inertial confinement fusion and assess its potential as an energy source. The NIF project involves a collaboration among the Lawrence Livermore National Laboratory (LLNL), Los Alamos National Laboratory (LANL), Sandia National Laboratory (SNL), and the University of Rochester's Laboratory for Laser Energetics (UR/LLE). In this paper, the role of the University of Rochester in the research, development, and planning required to assure the success of the NIF will be presented. The principal roles of the UR/LLE in the NIF are (1) validation of the direct-drive approach to NIF using the OMEGA 60-beam, 40-kJ UV laser facility; (2) support of indirect-drive physics experiments using OMEGA in collaboration with LLNL and LANL; (3) development of plasma diagnostics for NIF; (4) development of beam-smoothing techniques; and (5) development of thin-film coatings for NIF and cryogenic-fuel-layer targets for eventual application to NIF. 3 refs., 6 figs

Normalized impact factor (NIF): an adjusted method for calculating the citation rate of biomedical journals.

Science.gov (United States)

Owlia, P; Vasei, M; Goliaei, B; Nassiri, I

2011-04-01

The interests in journal impact factor (JIF) in scientific communities have grown over the last decades. The JIFs are used to evaluate journals quality and the papers published therein. JIF is a discipline specific measure and the comparison between the JIF dedicated to different disciplines is inadequate, unless a normalization process is performed. In this study, normalized impact factor (NIF) was introduced as a relatively simple method enabling the JIFs to be used when evaluating the quality of journals and research works in different disciplines. The NIF index was established based on the multiplication of JIF by a constant factor. The constants were calculated for all 54 disciplines of biomedical field during 2005, 2006, 2007, 2008 and 2009 years. Also, ranking of 393 journals in different biomedical disciplines according to the NIF and JIF were compared to illustrate how the NIF index can be used for the evaluation of publications in different disciplines. The findings prove that the use of the NIF enhances the equality in assessing the quality of research works produced by researchers who work in different disciplines. Copyright © 2010 Elsevier Inc. All rights reserved.

COATING AND MANDREL EFFECTS ON FABRICATION OF GLOW DISCHARGE POLYMER NIF SCALE INDIRECT DRIVE CAPSULES

International Nuclear Information System (INIS)

NIKROO, A.; PONTELANDOLFO, J.M.; CASTILLO, E.R.

2002-01-01

OAK A271 COATING AND MANDREL EFFECTS ON FABRICATION OF GLOW DISCHARGE POLYMER NIF SCALE INDIRECT DRIVE CAPSULES. Targets for the National Ignition Facility (NIF) need to be about 200 (micro)m thick and 2 mm in diameter. These dimensions are well beyond those currently fabricated on a routine basis. They have investigated fabrication of near NIF scale targets using the depolymerizable mandrel technique. Poly-alpha-methylstyrene (PAMS) mandrels, about 2 mm in diameter, of varying qualities were coated with as much as 125 (micro)m of glow discharge polymer (GDP). The surface finish of the final shells was examined using a variety of techniques. A clear dependence of the modal spectrum of final GDP shell on the quality of the initial PAMS mandrels was observed. isolated features were found to be the greatest cause for a shell not meeting the NIF standard

Gain measurements on a prototype NIF/LMJ amplifier pump cavity

International Nuclear Information System (INIS)

Rotter, M.D.; McCracken, R.; Erlandson, A.; Guenet, M.

1996-12-01

We are currently developing large-aperture amplifiers for the National Ignition Facility (NIF) and Laser Megajoules (LMJ) lasers. These multisegment amplifiers are of the flashlamp-pumped, Nd:Glass qW and are designed to propagate a nominally 36 cm square beam. The apertures within a particular amplifier bundle are arranged in a four-high by two-wide configuration and utilize two side lamp arrays and a central flashlamp array for pumping. The configuration is very similar to that used in the Beamlet laser, a single-beam prototype for the NIF/LMJ lasers, which has four apertures arranged in a two- high by two-wide configuration

Atomic and Molecular Data Activities at NIFS in 2009 - 2011

International Nuclear Information System (INIS)

Murakami, I.

2011-01-01

We open and maintain the NIFS atomic and molecular numerical databases. Numbers of data records increase to 476,048 in total (as of Aug. 23, 2011) and mainly new data are added for AMDIS (electron impact ionization, excitation, and recombination cross sections and rate coefficients) and CHART (charge transfer of atom - ion collisions cross sections) during last two years. A collaboration group has started for research on atomic and molecular processes in plasma using the Large Helical Device and we measure visible and extreme ultraviolet spectra of W and rare earth elements. We also organize a collaboration group with atomic physicists from Japanese universities for research on W to study atomic data, spectra and collisional-radiative models for W ions. (author)

Fnr is involved in oxygen control of Herbaspirillum seropedicae N-truncated NifA protein activity in Escherichia coli.

Science.gov (United States)

Monteiro, Rose A; de Souza, Emanuel M; Yates, M Geoffrey; Pedrosa, Fabio O; Chubatsu, Leda S

2003-03-01

Herbaspirillum seropedicae is an endophytic diazotroph belonging to the beta-subclass of the class Proteobacteria, which colonizes many members of the Gramineae. The activity of the NifA protein, a transcriptional activator of nif genes in H. seropedicae, is controlled by ammonium ions through its N-terminal domain and by oxygen through mechanisms that are not well understood. Here we report that the NifA protein of H. seropedicae is inactive and more susceptible to degradation in an fnr Escherichia coli background. Both effects correlate with oxygen exposure and iron deprivation. Our results suggest that the oxygen sensitivity and iron requirement for H. seropedicae NifA activity involve the Fnr protein.

Enriching project organizations with formal change agents

DEFF Research Database (Denmark)

Eskerod, Pernille; Justesen, Just Bendix; Sjøgaard, Gisela

2017-01-01

Purpose: Project success requires effective and efficient cooperation between the project organization and the permanent organization in which the project takes place. The purpose of this paper is to discuss potentials and pitfalls from enriching project organizations by appointing peers as formal...... and middle and top management support are major determinants of success within change projects. To select change agents that the employees respect and can identify with, combined with top management prioritization, is important in order for the project organization to benefit from the additional role...... change agents. Design/methodology/approach: The paper is based on a literature review and a multiple-case study in which six organizations participated in an action-oriented research project. The aim for the organizations was to obtain a better health status among the employees by accomplishing...

Molecular characterization of Azotobacter spp. nifH gene Isolated ...

African Journals Online (AJOL)

STORAGESEVER

2009-12-15

Dec 15, 2009 ... Available online at http://www.academicjournals.org/AJB. ISSN 1684–5315 ... Molecular characterization of Azotobacter spp. nifH .... MATERIALS AND METHODS ..... rapidly expanding and is currently composed of over.

Progress on establishing guidelines for National Ignition Facility (NIF) experiments to extend debris shield lifetime

International Nuclear Information System (INIS)

Tobin, M.; Eder, D.; Braun, D.; MacGowan, B.

2002-01-01

The survivability of the debris shields on the National Ignition Facility (NIF) are a key factor for the affordable operation of the facility. The improvements required over Nova debris shields are described. Estimates of debris shield lifetimes in the presence of target emissions with 4-8 J/cm 2 laser fluences indicate lifetimes that may contribute unacceptably to operations costs for NIF. We are developing detailed suggested guidance for target and experiment designers for NIF to assist in minimizing the damage to, and therefore the cost of, maintaining NIF debris shields. The guidance suggests a target mass quantity that as particulate on the debris shields (300 mg) may be within current operating budgets. It also suggests the amount of material that should become shrapnel on a shot (10 mg). Finally, it suggests the level of non-volatile residue (NVR) that would threaten the sol-gel coatings on the debris shields (1 μg/cm 2 ). We review the experimentation on the Nova chamber that included measuring quantities of particulate on debris shields by element and capturing shrapnel pieces in aerogel samples mounted in the chamber. We also describe computations of X-ray emissions from a likely NIF target and the associated ablation expected from this X-ray exposure on supporting target hardware. We describe progress in assessing the benefits of a pre-shield and the possible impact on the guidance for target experiments on NIF. Plans for possible experimentation on Omega and other facilities to improve our understanding of target emissions and their impacts are discussed. Our discussion of planned future work provides a forum to invite possible collaboration with the IFE community

D₂ and DT Liquid-Layer Target Shots on NIF

Energy Technology Data Exchange (ETDEWEB)

Walters, Curtis [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Alger, Ethan [General Atomics, San Diego, CA (United States); Bhandarkar, Suhas [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boehm, Kurt [General Atomics, San Diego, CA (United States); Braun, Tom [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Espinosaloza, Francisco [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Haid, Benjamin [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Heredia, Ricardo [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kline, John [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kozioziemski, Bernard [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kroll, Jeremy [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Malone, Daniel [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Nikroo, Abbas [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Opsahl, Patrick [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sater, James [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Zylstra, Alex [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-09-22

Experiments at the National Ignition Facility (NIF) using targets containing a Deuterium-Tritium (DT) fuel layer have, until recently, required that a high-quality layer of solid deuterium-tritium (herein referred to as an "ice-layer") be formed in the capsule. The development of a process to line the inner surface of a target capsule with a foam layer of a thickness that is typical of icelayers has resulted in the ability to field targets with liquid layers wetting the foam. Successful fielding of liquid-layer targets on NIF required not only a foam lined capsule, but also changes to the capsule filling process and the manner with which the inventory is maintained in the capsule. Additionally, changes to target heater power and the temperature drops across target components were required in order to achieve the desired range of shot temperatures. These changes, and the target's performance during four target shots on NIF will be discussed.

Annual report of National Institute for Fusion Science. April 2011 - March 2012

International Nuclear Information System (INIS)

2012-01-01

This annual report summarizes the research activities at NIFS (the National Institute for Fusion Science) between April 2011 and March 2012. NIFS is pursuing the integration of science and technology to realize a fusion power plant. The systematization of plasma physics, and research and development of reactor relevant engineering are key elements in our strategy. NIFS has been exploiting its role as an inter-university research organization and executing a variety of excellent collaborating studies together with universities and research institutes abroad as well as in Japan. The major projects of NIFS are the Large Helical Device (LHD) Project, the Numerical Simulation Research Project, the Fusion Engineering Research Project and the Coordination Research Project. These major projects are accompanied by unique supporting research. Advanced engineering and fusion reactor design studies are strongly promoted. (J.P.N.)

Annual report of National Institute for Fusion Science. April 2009 - March 2010

International Nuclear Information System (INIS)

2010-01-01

This annual report summarizes the research activities at NIFS (the National Institute for Fusion Science) between April 2009 and March 2010. NIFS is pursuing the integration of science and technology to realize a fusion power plant. The systematization of plasma physics, and research and development of reactor relevant engineering are key elements in our strategy. NIFS has been exploiting its role as an inter-university research organization and executing a variety of excellent collaborating studies together with universities and research institutes abroad as well as in Japan. The major projects of NIFS are the Large Helical Device (LHD) Project, the Numerical Simulation Research Project, the Fusion Engineering Research Project and the Coordination Research Project. These major projects are accompanied by unique supporting research. Advanced engineering and fusion reactor design studies are strongly promoted. (J.P.N.)

Annual report of National Institute for Fusion Science. April 2012 - March 2013

International Nuclear Information System (INIS)

2013-01-01

This annual report summarizes the research activities at NIFS (the National Institute for Fusion Science) between April 2012 and March 2013. NIFS is pursuing the integration of science and technology to realize a fusion power plant. The systematization of plasma physics, and research and development of reactor relevant engineering are key elements in our strategy. NIFS has been exploiting its role as an inter-university research organization and executing a variety of excellent collaborating studies together with universities and research institutes abroad as well as in Japan. The major projects of NIFS are the Large Helical Device (LHD) Project, the Numerical Simulation Research Project, the Fusion Engineering Research Project and the Coordination Research Project. These major projects are accompanied by unique supporting research. Advanced engineering and fusion reactor design studies are strongly promoted. (J.P.N.)

Laser coupling to reduced-scale targets at Nif Early Light

Energy Technology Data Exchange (ETDEWEB)

Hinkel, D.E.; Schneider, M.B.; Young, B.K.; Holder, J.P.; Langdon, A.B.; Bonanno, G.; Bower, D.E.; Bruns, H.C.; Campbell, K.M.; Celeste, J.R.; Compton, S.; Costa, R.L.; Dewald, E.L.; Dixit, S.N.; Eckart, M.J.; Eder, D.C.; Edwards, M.J.; Ellis, A.D.; Emig, J.A.; Froula, D.H.; Glenzer, S.H.; Hargrove, D.; Haynam, C.A.; Heeter, R.F.; Henesian, M.A.; Holtmeier, G.; James, D.L.; Jancaitis, K.S.; Kalantar, K.H.; Kamperschroer, J.H.; Kauffman, R.L.; Kimbrough, J.; Kirkwood, R.K.; Koniges, A.E.; Landen, O.L.; Landon, M.; Lee, F.D.; MacGowan, B.J.; Mackinnon, A.J.; Manes, K.R.; Marshall, C.; May, M.J.; McDonald, J.W.; Menapace, J.; Moses, S.E.I.; Munro, D.H.; Murray, J.R.; Niemann, C.; Power, G.D.; Rekow, V.; Ruppe, J.A.; Schein, J.; Shepherd, R.; Singh, M.S.; Springer, P.T.; Still, C.H.; Suter, L.J.; Tietbohl, G.L.; Turner, R.E.; VanWonterghem, B.M.; Wallace, R.J.; Warrick, A.; Weber, F.; Wegner, P.J.; Williams, E.A.; Young, P.E. [Lawrence Livermore National Laboratory, Livermore, CA (United States); Baldis, H.A. [California at Davis Univ., CA (United States); Pellinen, D.; Watts, P. [Bechtel Nevada Corporation, Livermore, CA (United States)

2006-06-15

Deposition of maximum laser energy into a small, high-Z enclosure in a short laser pulse creates a hot environment. Such targets were recently included in an experimental campaign using the first four of the 192 beams of the National Ignition Facility (NIF) under construction at the University of California Lawrence Livermore National Laboratory. These targets demonstrate good laser coupling, reaching a radiation temperature of 340 eV. In addition, the Raman backscatter spectrum contains features consistent with Brillouin backscatter of Raman forward scatter. Also, NIF Early Light diagnostics indicate that 20% of the direct backscatter from these reduced-scale targets is in the polarization orthogonal to that of the incident light. (authors)

NIF small optics laser damage test specifications

International Nuclear Information System (INIS)

Sheehan, L

1999-01-01

The Laser Damage Group is currently conducting tests on small optics samples supplied for initial evaluation of potential NIF suppliers. This document is meant to define the specification of laser-induced damage for small optics and the test methods used to collect the data. A rating system which will be applied for vendor selection is presented

NIFS contributions to 19th IAEA fusion energy conference

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-11-01

NIFS has presented 21 papers at the 19th IAEA Fusion Energy Conference (Lyon, France, 14-19 October 2002). The contributed papers are collected in this report. The 21 papers are indexed individually. (J.P.N.)

Evidence for high-temperature in situ nifH transcription in an alkaline hot spring of Lower Geyser Basin, Yellowstone National Park.

Science.gov (United States)

Loiacono, Sara T; Meyer-Dombard, D'Arcy R; Havig, Jeff R; Poret-Peterson, Amisha T; Hartnett, Hilairy E; Shock, Everett L

2012-05-01

Genes encoding nitrogenase (nifH) were amplified from sediment and photosynthetic mat samples collected in the outflow channel of Mound Spring, an alkaline thermal feature in Yellowstone National Park. Results indicate the genetic capacity for nitrogen fixation over the entire range of temperatures sampled (57.2°C to 80.2°C). Amplification of environmental nifH transcripts revealed in situ expression of nifH genes at temperatures up to 72.7°C. However, we were unable to amplify transcripts of nifH at the higher-temperature locations (> 72.7°C). These results indicate that microbes at the highest temperature sites contain the genetic capacity to fix nitrogen, yet either do not express nifH or do so only transiently. Field measurements of nitrate and ammonium show fixed nitrogen limitation as temperature decreases along the outflow channel, suggesting nifH expression in response to the downstream decrease in bioavailable nitrogen. Nitrogen stable isotope values of Mound Spring sediment communities further support geochemical and genetic data. DNA and cDNA nifH amplicons form several unique phylogenetic clades, some of which appear to represent novel nifH sequences in both photosynthetic and chemosynthetic microbial communities. This is the first report of in situ nifH expression in strictly chemosynthetic zones of terrestrial (non-marine) hydrothermal systems, and sets a new upper temperature limit (72.7°C) for nitrogen fixation in alkaline, terrestrial hydrothermal environments. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Project-based knowledge in organizing open innovation

CERN Document Server

Comacchio, Anna; Pizzi, Claudio

2014-01-01

Enriching understanding of the current theoretical debate on project-based open innovation, ‘Project-based Knowledge in Organizing Open Innovation’ draws on innovation management literature and knowledge-based perspectives to investigate the relationship between knowledge development at project level and the strategic organization of open innovation. Addressing the still open issue of how the firm level should be complemented by studies at the project level of analysis, this book provides theoretical and empirical arguments on the advantages of a more fine-grained level of analysis to understand how firms organize their innovation processes across boundaries. The book also addresses the emerging interest in the management literature on project-based organizations, and on the relevance of project forms of organizing in a knowledge-based economy. Through field research in different industrial settings , this book provides empirical evidence on how firms design open innovation project-by-project and it will ...

In silico characterization and transcriptomic analysis of nif family genes from Anabaena sp. PCC7120.

Science.gov (United States)

Singh, Shilpi; Shrivastava, Alok Kumar

2017-10-01

In silico approaches in conjunction with morphology, nitrogenase activity, and qRT-PCR explore the impact of selected abiotic stressor such as arsenic, salt, cadmium, copper, and butachlor on nitrogen fixing (nif family) genes of diazotrophic cyanobacterium Anabaena sp. PCC7120. A total of 19 nif genes are present within the Anabaena genome that is involved in the process of nitrogen fixation. Docking studies revealed the interaction between these nif gene-encoded proteins and the selected abiotic stressors which were further validated through decreased heterocyst frequency, fragmentation of filaments, and downregulation of nitrogenase activity under these stresses indicating towards their toxic impact on nitrogen fixation potential of filamentous cyanobacterium Anabaena sp. PCC7120. Another appealing finding of this study is even though having similar binding energy and similar interacting residues between arsenic/salt and copper/cadmium to nif-encoded proteins, arsenic and cadmium are more toxic than salt and copper for nitrogenase activity of Anabaena which is crucial for growth and yield of rice paddy and soil reclamation.

The NifA-RpoN regulon of Mesorhizobium loti strain R7A and its symbiotic activation by a novel LacI/GalR-family regulator.

Directory of Open Access Journals (Sweden)

John T Sullivan

Full Text Available Mesorhizobium loti is the microsymbiont of Lotus species, including the model legume L. japonicus. M. loti differs from other rhizobia in that it contains two copies of the key nitrogen fixation regulatory gene nifA, nifA1 and nifA2, both of which are located on the symbiosis island ICEMlSym(R7A. M. loti R7A also contains two rpoN genes, rpoN1 located on the chromosome outside of ICEMlSym(R7A and rpoN2 that is located on ICEMlSym(R7A. The aims of the current work were to establish how nifA expression was activated in M. loti and to characterise the NifA-RpoN regulon. The nifA2 and rpoN2 genes were essential for nitrogen fixation whereas nifA1 and rpoN1 were dispensable. Expression of nifA2 was activated, possibly in response to an inositol derivative, by a novel regulator of the LacI/GalR family encoded by the fixV gene located upstream of nifA2. Other than the well-characterized nif/fix genes, most NifA2-regulated genes were not required for nitrogen fixation although they were strongly expressed in nodules. The NifA-regulated nifZ and fixU genes, along with nifQ which was not NifA-regulated, were required in M. loti for a fully effective symbiosis although they are not present in some other rhizobia. The NifA-regulated gene msi158 that encodes a porin was also required for a fully effective symbiosis. Several metabolic genes that lacked NifA-regulated promoters were strongly expressed in nodules in a NifA2-dependent manner but again mutants did not have an overt symbiotic phenotype. In summary, many genes encoded on ICEMlSym(R7A were strongly expressed in nodules but not free-living rhizobia, but were not essential for symbiotic nitrogen fixation. It seems likely that some of these genes have functional homologues elsewhere in the genome and that bacteroid metabolism may be sufficiently plastic to adapt to loss of certain enzymatic functions.

Current progress in NIF target concepts

International Nuclear Information System (INIS)

Gobby, P.L.; Foreman, L.R.; Thoma, D.J.; Jacobson, L.A.; Hollis, R.V.; Barrera, J.; Mitchell, M.A.; Salazar, M.A.; Salzer, L.J.

1996-01-01

Target concepts for the National Ignition Facility (NIF) require progress in the art and science of target fabrication. Three distinct issues are addressed: beryllium fuel capsules, foam-buffered direct drive, and high-density gas-filled hohlraums. In all cases experiments on the existing Nova laser at LLNL are either in progress or planned for the near future to test the various concepts. Consequently, target fabrication must be able to deliver targets appropriate for each

Sensorimotor nucleus NIf is necessary for auditory processing but not vocal motor output in the avian song system.

Science.gov (United States)

Cardin, Jessica A; Raksin, Jonathan N; Schmidt, Marc F

2005-04-01

Sensorimotor integration in the avian song system is crucial for both learning and maintenance of song, a vocal motor behavior. Although a number of song system areas demonstrate both sensory and motor characteristics, their exact roles in auditory and premotor processing are unclear. In particular, it is unknown whether input from the forebrain nucleus interface of the nidopallium (NIf), which exhibits both sensory and premotor activity, is necessary for both auditory and premotor processing in its target, HVC. Here we show that bilateral NIf lesions result in long-term loss of HVC auditory activity but do not impair song production. NIf is thus a major source of auditory input to HVC, but an intact NIf is not necessary for motor output in adult zebra finches.

The NIF LinkOut broker: a web resource to facilitate federated data integration using NCBI identifiers.

Science.gov (United States)

Marenco, Luis; Ascoli, Giorgio A; Martone, Maryann E; Shepherd, Gordon M; Miller, Perry L

2008-09-01

This paper describes the NIF LinkOut Broker (NLB) that has been built as part of the Neuroscience Information Framework (NIF) project. The NLB is designed to coordinate the assembly of links to neuroscience information items (e.g., experimental data, knowledge bases, and software tools) that are (1) accessible via the Web, and (2) related to entries in the National Center for Biotechnology Information's (NCBI's) Entrez system. The NLB collects these links from each resource and passes them to the NCBI which incorporates them into its Entrez LinkOut service. In this way, an Entrez user looking at a specific Entrez entry can LinkOut directly to related neuroscience information. The information stored in the NLB can also be utilized in other ways. A second approach, which is operational on a pilot basis, is for the NLB Web server to create dynamically its own Web page of LinkOut links for each NCBI identifier in the NLB database. This approach can allow other resources (in addition to the NCBI Entrez) to LinkOut to related neuroscience information. The paper describes the current NLB system and discusses certain design issues that arose during its implementation.

Near Field Intensity Trends of Main Laser Alignment Images in the National Ignition Facility (NIF)

Energy Technology Data Exchange (ETDEWEB)

Leach, R R; Beltsar, I; Burkhart, S; Lowe-Webb, R; Kamm, V M; Salmon, T; Wilhelmsen, K

2015-01-22

The National Ignition Facility (NIF) utilizes 192 high-energy laser beams focused with enough power and precision on a hydrogen-filled spherical, cryogenic target to potentially initiate a fusion reaction. NIF has been operational for six years; during that time, thousands of successful laser firings or shots have been executed. Critical instrument measurements and camera images are carefully recorded for each shot. The result is a massive and complex database or ‘big data’ archive that can be used to investigate the state of the laser system at any point in its history or to locate and track trends in the laser operation over time. In this study, the optical light throughput for more than 1600 NIF shots for each of the 192 main laser beams and 48 quads was measured over a three year period from January 2009 to October 2012. The purpose was to verify that the variation in the transmission of light through the optics over time performed within design expectations during this time period. Differences between average or integrated intensity from images recorded by the input sensor package (ISP) and by the output sensor package (OSP) in the NIF beam-line were examined. A metric is described for quantifying changes in the integrated intensity measurements and was used to view potential trends. Results are presented for the NIF input and output sensor package trends and changes over the three year time-frame.

Plasma Electrode Pockels Cells for the Beamlet and NIF lasers

International Nuclear Information System (INIS)

Rhodes, M.A.; Woods, B.; DeYoreo, J.; Atherton, J.

1994-05-01

We describe Plasma Electrode Pockels Cells (PEPC) for the Beamlet laser and the proposed National Ignition Facility (NIF) laser. These PEPCs, together with passive polarizers, function as large aperture (> 35 x 35 cm 2 ) optical switches enabling the design of high-energy (> 5 kJ), multipass laser amplifiers. In a PEPC, plasma discharges form on both sides of a thin (1 cm) electro-optic crystal (KDP). These plasma discharges produce highly conductive and transparent electrodes that facilitate rapid (< 100 ns) and uniform charging of the KDP up to the half-wave voltage (17 kV) and back to zero volts. We discuss the operating principles, design, and optical performance of the Beamlet PEPC and briefly discuss our plans to extend PEPC technology for the NIF

The Design of Project Management Structural Organization

OpenAIRE

Dumitru Constantinescu; Cristian Etegan

2007-01-01

The relationships organization-suppliers-customers have recently known major changes in the structure of services and have made the organization develop its managerial and professional competencies in order to do projects. The qualified organization is the most trust-worthy in the process of doing a project. The participation of an organization in doing projects depends on a multitude of factors. Out of these factors, the structural organization comes forth, as it represents the variable with...

Functional NifD-K fusion protein in Azotobacter vinelandii is a homodimeric complex equivalent to the native heterotetrameric MoFe protein

International Nuclear Information System (INIS)

Lahiri, Surobhi; Pulakat, Lakshmi; Gavini, Nara

2005-01-01

The MoFe protein of the complex metalloenzyme nitrogenase folds as a heterotetramer containing two copies each of the homologous α and β subunits, encoded by the nifD and the nifK genes respectively. Recently, the functional expression of a fusion NifD-K protein of nitrogenase was demonstrated in Azotobacter vinelandii, strongly implying that the MoFe protein is flexible as it could accommodate major structural changes, yet remain functional [M.H. Suh, L. Pulakat, N. Gavini, J. Biol. Chem. 278 (2003) 5353-5360]. This finding led us to further explore the type of interaction between the fused MoFe protein units. We aimed to determine whether an interaction exists between the two fusion MoFe proteins to form a homodimer that is equivalent to native heterotetrameric MoFe protein. Using the Bacteriomatch Two-Hybrid System, translationally fused constructs of NifD-K (fusion) with the full-length λCI of the pBT bait vector and also NifD-K (fusion) with the N-terminal α-RNAP of the pTRG target vector were made. To compare the extent of interaction between the fused NifD-K proteins to that of the β-β interactions in the native MoFe protein, we proceeded to generate translationally fused constructs of NifK with the α-RNAP of the pTRG vector and λCI protein of the pBT vector. The strength of the interaction between the proteins in study was determined by measuring the β-galactosidase activity and extent of ampicillin resistance of the colonies expressing these proteins. This analysis demonstrated that direct protein-protein interaction exists between NifD-K fusion proteins, suggesting that they exist as homodimers. As the interaction takes place at the β-interfaces of the NifD-K fusion proteins, we propose that these homodimers of NifD-K fusion protein may function in a similar manner as that of the heterotetrameric native MoFe protein. The observation that the extent of protein-protein interaction between the β-subunits of the native MoFe protein in Bacterio

The neutron imaging diagnostic at NIF (invited).

Science.gov (United States)

Merrill, F E; Bower, D; Buckles, R; Clark, D D; Danly, C R; Drury, O B; Dzenitis, J M; Fatherley, V E; Fittinghoff, D N; Gallegos, R; Grim, G P; Guler, N; Loomis, E N; Lutz, S; Malone, R M; Martinson, D D; Mares, D; Morley, D J; Morgan, G L; Oertel, J A; Tregillis, I L; Volegov, P L; Weiss, P B; Wilde, C H; Wilson, D C

2012-10-01

A neutron imaging diagnostic has recently been commissioned at the National Ignition Facility (NIF). This new system is an important diagnostic tool for inertial fusion studies at the NIF for measuring the size and shape of the burning DT plasma during the ignition stage of Inertial Confinement Fusion (ICF) implosions. The imaging technique utilizes a pinhole neutron aperture, placed between the neutron source and a neutron detector. The detection system measures the two dimensional distribution of neutrons passing through the pinhole. This diagnostic has been designed to collect two images at two times. The long flight path for this diagnostic, 28 m, results in a chromatic separation of the neutrons, allowing the independently timed images to measure the source distribution for two neutron energies. Typically the first image measures the distribution of the 14 MeV neutrons and the second image of the 6-12 MeV neutrons. The combination of these two images has provided data on the size and shape of the burning plasma within the compressed capsule, as well as a measure of the quantity and spatial distribution of the cold fuel surrounding this core.

The neutron imaging diagnostic at NIF (invited)

Energy Technology Data Exchange (ETDEWEB)

Merrill, F. E.; Clark, D. D.; Danly, C. R.; Drury, O. B.; Fatherley, V. E.; Gallegos, R.; Grim, G. P.; Guler, N.; Loomis, E. N.; Martinson, D. D.; Mares, D.; Morley, D. J.; Morgan, G. L.; Oertel, J. A.; Tregillis, I. L.; Volegov, P. L.; Wilde, C. H.; Wilson, D. C. [Los Alamos National Laboratory, Los Alamos, New Mexico 87544 (United States); Bower, D.; Dzenitis, J. M. [Livermore National Laboratory, Livermore, California 94550 (United States); and others

2012-10-15

A neutron imaging diagnostic has recently been commissioned at the National Ignition Facility (NIF). This new system is an important diagnostic tool for inertial fusion studies at the NIF for measuring the size and shape of the burning DT plasma during the ignition stage of Inertial Confinement Fusion (ICF) implosions. The imaging technique utilizes a pinhole neutron aperture, placed between the neutron source and a neutron detector. The detection system measures the two dimensional distribution of neutrons passing through the pinhole. This diagnostic has been designed to collect two images at two times. The long flight path for this diagnostic, 28 m, results in a chromatic separation of the neutrons, allowing the independently timed images to measure the source distribution for two neutron energies. Typically the first image measures the distribution of the 14 MeV neutrons and the second image of the 6-12 MeV neutrons. The combination of these two images has provided data on the size and shape of the burning plasma within the compressed capsule, as well as a measure of the quantity and spatial distribution of the cold fuel surrounding this core.

Managing NIF safety equipment in a high neutron and gamma radiation environment.

Science.gov (United States)

Datte, Philip; Eckart, Mark; Jackson, Mark; Khater, Hesham; Manuel, Stacie; Newton, Mark

2013-06-01

The National Ignition Facility (NIF) is a 192 laser beam facility that supports the Inertial Confinement Fusion program. During the ignition experimental campaign, the NIF is expected to perform shots with varying fusion yield producing 14 MeV neutrons up to 20 MJ or 7.1 × 10(18) neutrons per shot and a maximum annual yield of 1,200 MJ. Several infrastructure support systems will be exposed to varying high yield shots over the facility's 30-y life span. In response to this potential exposure, analysis and testing of several facility safety systems have been conducted. A detailed MCNP (Monte Carlo N-Particle Transport Code) model has been developed for the NIF facility, and it includes most of the major structures inside the Target Bay. The model has been used in the simulation of expected neutron and gamma fluences throughout the Target Bay. Radiation susceptible components were identified and tested to fluences greater than 10(13) (n cm(-2)) for 14 MeV neutrons and γ-ray equivalent. The testing includes component irradiation using a 60Co gamma source and accelerator-based irradiation using 4- and 14- MeV neutron sources. The subsystem implementation in the facility is based on the fluence estimates after shielding and survivability guidelines derived from the dose maps and component tests results. This paper reports on the evaluation and implementation of mitigations for several infrastructure safety support systems, including video, oxygen monitoring, pressure monitors, water sensing systems, and access control interfaces found at the NIF.

IGNITOR, ITER and NIF in the Context of the World Effort on Fusion Burning Plasmas

Science.gov (United States)

Azizov, E.; Coppi, B.; Velikhov, E.

2012-03-01

As of last summer, the ITER program has been recognized as being directed at providing an ``International Platform for Fusion Technology.'' Then, the two experimental programs that have the explicit goal to approach ignition conditions with D-T plasmas are NIF and IGNITOR. NIF, the National Ignition Facility, is based on the inertial confinement principle using a laser system capable of delivering 1.6 MJ and is being operated in Livermore. IGNITOR will be operated by the Kurchatov Institute within the research center of Troitzk presently owned by Rosatom and involves a high level collaboration between Italy and Russia. For this, Ignitor has been defined as a Flagship Project by Italy and the construction of its core has been funded. The Ignitor design is based on the experimental results obtained by the high field line of experiments carried out at MIT, within the Alcator Program, and in Italy within the Frascati Torus Program. A wide set of experiments in Japan, on high density plasmas, in the US, Russia and Europe have produced plasma physics results and technology developments that have guided the evolution of the Ignitor design. The main theoretical plasma physics issues to be dealt with in connection with this program are discussed.

NIF capsule performance modeling

Directory of Open Access Journals (Sweden)

Weber S.

2013-11-01

Full Text Available Post-shot modeling of NIF capsule implosions was performed in order to validate our physical and numerical models. Cryogenic layered target implosions and experiments with surrogate targets produce an abundance of capsule performance data including implosion velocity, remaining ablator mass, times of peak x-ray and neutron emission, core image size, core symmetry, neutron yield, and x-ray spectra. We have attempted to match the integrated data set with capsule-only simulations by adjusting the drive and other physics parameters within expected uncertainties. The simulations include interface roughness, time-dependent symmetry, and a model of mix. We were able to match many of the measured performance parameters for a selection of shots.

Crystallization and preliminary crystallographic data of the PAS domain of the NifL protein from Azotobacter vinelandii.

NARCIS (Netherlands)

Hefti, M.H.; Hendle, J.; Enroth, C.; Vervoort, J.J.M.; Tucker, P.A.

2001-01-01

The Azotobacter vinelandii NifL protein is a redox-sensing flavoprotein which inhibits the activity of the nitrogen-specific transcriptional activator NifA. The N-terminal PAS domain has been overexpressed in Escherichia coli and crystallized by the hanging-drop vapour-diffusion method. The crystal

Symbiotic Burkholderia Species Show Diverse Arrangements of nif/fix and nod Genes and Lack Typical High-Affinity Cytochrome cbb3 Oxidase Genes.

Science.gov (United States)

De Meyer, Sofie E; Briscoe, Leah; Martínez-Hidalgo, Pilar; Agapakis, Christina M; de-Los Santos, Paulina Estrada; Seshadri, Rekha; Reeve, Wayne; Weinstock, George; O'Hara, Graham; Howieson, John G; Hirsch, Ann M

2016-08-01

Genome analysis of fourteen mimosoid and four papilionoid beta-rhizobia together with fourteen reference alpha-rhizobia for both nodulation (nod) and nitrogen-fixing (nif/fix) genes has shown phylogenetic congruence between 16S rRNA/MLSA (combined 16S rRNA gene sequencing and multilocus sequence analysis) and nif/fix genes, indicating a free-living diazotrophic ancestry of the beta-rhizobia. However, deeper genomic analysis revealed a complex symbiosis acquisition history in the beta-rhizobia that clearly separates the mimosoid and papilionoid nodulating groups. Mimosoid-nodulating beta-rhizobia have nod genes tightly clustered in the nodBCIJHASU operon, whereas papilionoid-nodulating Burkholderia have nodUSDABC and nodIJ genes, although their arrangement is not canonical because the nod genes are subdivided by the insertion of nif and other genes. Furthermore, the papilionoid Burkholderia spp. contain duplications of several nod and nif genes. The Burkholderia nifHDKEN and fixABC genes are very closely related to those found in free-living diazotrophs. In contrast, nifA is highly divergent between both groups, but the papilionoid species nifA is more similar to alpha-rhizobia nifA than to other groups. Surprisingly, for all Burkholderia, the fixNOQP and fixGHIS genes required for cbb3 cytochrome oxidase production and assembly are missing. In contrast, symbiotic Cupriavidus strains have fixNOQPGHIS genes, revealing a divergence in the evolution of two distinct electron transport chains required for nitrogen fixation within the beta-rhizobia.

Managing a project's legacy: implications for organizations and project management

Science.gov (United States)

Cooper, Lynne P.; Hecht, Michael H.; Majchrzak, Ann

2003-01-01

Organizations that rely on projects to implement their products must find effective mechanisms for propagating lessons learned on one project throughout the organization. A broad view of what constitutes a project's 'legacy' is presented that includes not just the design products and leftover parts, but new processes, relationships, technology, skills, planning data, and performance metrics. Based on research evaluating knowledge reuse in innovative contexts, this paper presents an approach to project legacy management that focuses on collecting and using legacy knowledge to promote organizational learning and effective reuse, while addressing factors of post-project responsibility, information obsolescence, and the importance of ancillary contextual information. .

Design calculations for NIF convergent ablator experiments

Directory of Open Access Journals (Sweden)

Olson R.E.

2013-11-01

Full Text Available The NIF convergent ablation tuning effort is underway. In the early experiments, we have discovered that the design code simulations over-predict the capsule implosion velocity and shock flash ρr, but under-predict the hohlraum x-ray flux measurements. The apparent inconsistency between the x-ray flux and radiography data implies that there are important unexplained aspects of the hohlraum and/or capsule behavior.

An exploration of Lasnex designs to support the MShock campaign at NIF

Energy Technology Data Exchange (ETDEWEB)

DeVolder, Barbara Gloria [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-10-11

In support of planned MShock experiments at NIF, Lasnex is being used to explore possible laser sources and halfraum designs that launch two shocks into an ablator - shock tube configuration. Two design features were investigated: (1) launching a two-component laser source consisting of an early pre-pulse separated in time from a later main pulse, to create two shocks, and (2) adding a liner on the inner surface of the gold wall of the halfraum, to delay or prevent gold stagnation on-axis and to preserve the effectiveness of the second laser pulse. The clearest indication of two shocks propagating in the shock tube occurred when the pre-pulse mimicked a direct drive, achieved by re-pointing the inner beam cones, and when a liner was used to ensure that the free-electron number density was below the critical density for NIF. Additional simulations (presented in the Appendix) that used the two-component laser source but focused on specific choices in laser-pulse design space, as proposed by Kirk Flippo for use in the first MShock experiments at NIF, also demonstrated the presence of two shocks.

Measuring the absolute DT neutron yield using the Magnetic Recoil Spectrometer at OMEGA and the NIF

Energy Technology Data Exchange (ETDEWEB)

Mackinnon, A; Casey, D; Frenje, J A; Johnson, M G; Seguin, F H; Li, C K; Petrasso, R D; Glebov, V Y; Katz, J; Knauer, J; Meyerhofer, D; Sangster, T; Bionta, R; Bleuel, D; Hachett, S P; Hartouni, E; Lepape, S; Mckernan, M; Moran, M; Yeamans, C

2012-05-03

A Magnetic Recoil Spectrometer (MRS) has been installed and extensively used on OMEGA and the National Ignition Facility (NIF) for measurements of the absolute neutron spectrum from inertial confinement fusion (ICF) implosions. From the neutron spectrum measured with the MRS, many critical implosion parameters are determined including the primary DT neutron yield, the ion temperature, and the down-scattered neutron yield. As the MRS detection efficiency is determined from first principles, the absolute DT neutron yield is obtained without cross-calibration to other techniques. The MRS primary DT neutron measurements at OMEGA and the NIF are shown to be in excellent agreement with previously established yield diagnostics on OMEGA, and with the newly commissioned nuclear activation diagnostics on the NIF.

Nitrogen Fixation Aligns with nifH Abundance and Expression in Two Coral Trophic Functional Groups

KAUST Repository

Pogoreutz, Claudia; Radecker, Nils; Cardenas, Anny; Gä rdes, Astrid; Wild, Christian; Voolstra, Christian R.

2017-01-01

Microbial nitrogen fixation (diazotrophy) is a functional trait widely associated with tropical reef-building (scleractinian) corals. While the integral role of nitrogen fixation in coral nutrient dynamics is recognized, its ecological significance across different coral functional groups remains yet to be evaluated. Here we set out to compare molecular and physiological patterns of diazotrophy (i.e., nifH gene abundance and expression as well as nitrogen fixation rates) in two coral families with contrasting trophic strategies: highly heterotrophic, free-living members of the family Fungiidae (Pleuractis granulosa, Ctenactis echinata), and mostly autotrophic coral holobionts with low heterotrophic capacity (Pocilloporidae: Pocillopora verrucosa, Stylophora pistillata). The Fungiidae exhibited low diazotroph abundance (based on nifH gene copy numbers) and activity (based on nifH gene expression and the absence of detectable nitrogen fixation rates). In contrast, the mostly autotrophic Pocilloporidae exhibited nifH gene copy numbers and gene expression two orders of magnitude higher than in the Fungiidae, which coincided with detectable nitrogen fixation activity. Based on these data, we suggest that nitrogen fixation compensates for the low heterotrophic nitrogen uptake in autotrophic corals. Consequently, the ecological importance of diazotrophy in coral holobionts may be determined by the trophic functional group of the host.

Nitrogen Fixation Aligns with nifH Abundance and Expression in Two Coral Trophic Functional Groups

KAUST Repository

Pogoreutz, Claudia

2017-06-28

Microbial nitrogen fixation (diazotrophy) is a functional trait widely associated with tropical reef-building (scleractinian) corals. While the integral role of nitrogen fixation in coral nutrient dynamics is recognized, its ecological significance across different coral functional groups remains yet to be evaluated. Here we set out to compare molecular and physiological patterns of diazotrophy (i.e., nifH gene abundance and expression as well as nitrogen fixation rates) in two coral families with contrasting trophic strategies: highly heterotrophic, free-living members of the family Fungiidae (Pleuractis granulosa, Ctenactis echinata), and mostly autotrophic coral holobionts with low heterotrophic capacity (Pocilloporidae: Pocillopora verrucosa, Stylophora pistillata). The Fungiidae exhibited low diazotroph abundance (based on nifH gene copy numbers) and activity (based on nifH gene expression and the absence of detectable nitrogen fixation rates). In contrast, the mostly autotrophic Pocilloporidae exhibited nifH gene copy numbers and gene expression two orders of magnitude higher than in the Fungiidae, which coincided with detectable nitrogen fixation activity. Based on these data, we suggest that nitrogen fixation compensates for the low heterotrophic nitrogen uptake in autotrophic corals. Consequently, the ecological importance of diazotrophy in coral holobionts may be determined by the trophic functional group of the host.

Political hybrids: tocquevillian views on project organizations

NARCIS (Netherlands)

Clegg, S.R.; Courpasson, D.

2004-01-01

For the past decade, project organization has become increasingly central to management and organization studies, particularly as these seek to discern the contours of post-modern organizations. Yet, these contours frequently seem to be sighted without bearings on the current realities of project

Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial mat

DEFF Research Database (Denmark)

Steunou, Anne-Soisig; Jensen, Sheila I; Brecht, Eric

2008-01-01

Nitrogen fixation, a prokaryotic, O(2)-inhibited process that reduces N(2) gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle...... in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night...

Extending the NIF DISCO framework to automate complex workflow: coordinating the harvest and integration of data from diverse neuroscience information resources.

Science.gov (United States)

Marenco, Luis N; Wang, Rixin; Bandrowski, Anita E; Grethe, Jeffrey S; Shepherd, Gordon M; Miller, Perry L

2014-01-01

This paper describes how DISCO, the data aggregator that supports the Neuroscience Information Framework (NIF), has been extended to play a central role in automating the complex workflow required to support and coordinate the NIF's data integration capabilities. The NIF is an NIH Neuroscience Blueprint initiative designed to help researchers access the wealth of data related to the neurosciences available via the Internet. A central component is the NIF Federation, a searchable database that currently contains data from 231 data and information resources regularly harvested, updated, and warehoused in the DISCO system. In the past several years, DISCO has greatly extended its functionality and has evolved to play a central role in automating the complex, ongoing process of harvesting, validating, integrating, and displaying neuroscience data from a growing set of participating resources. This paper provides an overview of DISCO's current capabilities and discusses a number of the challenges and future directions related to the process of coordinating the integration of neuroscience data within the NIF Federation.

Measuring the absolute deuterium-tritium neutron yield using the magnetic recoil spectrometer at OMEGA and the NIF.

Science.gov (United States)

Casey, D T; Frenje, J A; Gatu Johnson, M; Séguin, F H; Li, C K; Petrasso, R D; Glebov, V Yu; Katz, J; Knauer, J P; Meyerhofer, D D; Sangster, T C; Bionta, R M; Bleuel, D L; Döppner, T; Glenzer, S; Hartouni, E; Hatchett, S P; Le Pape, S; Ma, T; MacKinnon, A; McKernan, M A; Moran, M; Moses, E; Park, H-S; Ralph, J; Remington, B A; Smalyuk, V; Yeamans, C B; Kline, J; Kyrala, G; Chandler, G A; Leeper, R J; Ruiz, C L; Cooper, G W; Nelson, A J; Fletcher, K; Kilkenny, J; Farrell, M; Jasion, D; Paguio, R

2012-10-01

A magnetic recoil spectrometer (MRS) has been installed and extensively used on OMEGA and the National Ignition Facility (NIF) for measurements of the absolute neutron spectrum from inertial confinement fusion implosions. From the neutron spectrum measured with the MRS, many critical implosion parameters are determined including the primary DT neutron yield, the ion temperature, and the down-scattered neutron yield. As the MRS detection efficiency is determined from first principles, the absolute DT neutron yield is obtained without cross-calibration to other techniques. The MRS primary DT neutron measurements at OMEGA and the NIF are shown to be in excellent agreement with previously established yield diagnostics on OMEGA, and with the newly commissioned nuclear activation diagnostics on the NIF.

Breakthrough at NIF 'unlikely' in 2010

Science.gov (United States)

Harris, Margaret

2010-05-01

Hopes of reaching a milestone in fusion research by the end of 2010 have dimmed following a US government report that plays down the chances of an early breakthrough and sharply criticizes management of the 4bn National Ignition Facility (NIF). In the report, officials from the Government Accountability Office (GAO) state that ignition - fusion's "break-even" point - is "unlikely" to occur at the laser-fusion lab this year and that "significant scientific and technical challenges" could delay or even prevent the facility from achieving ignition by 2012.

Technical documentation in support of the project-specific analysis for construction and operation of the National Ignition Facility

Energy Technology Data Exchange (ETDEWEB)

Lazaro, M.A.; Vinikour, W. [Argonne National Lab., IL (United States). Environmental Assessment Div.; Allison, T. [Argonne National Lab., IL (United States). Decision and Information Sciences Div.] [and others

1996-09-01

This document provides information that supports or supplements the data and impact analyses presented in the National Ignition Facility (NIF) Project-Specific Analysis (PSA). The purposes of NIF are to achieve fusion ignition in the laboratory for the first time with inertial confinement fusion (ICF) technology and to conduct high- energy-density experiments ins support of national security and civilian application. NIF is an important element in the DOE`s science-based SSM Program, a key mission of which is to ensure the reliability of the nation`s enduring stockpile of nuclear weapons. NIF would also advance the knowledge of basic and applied high-energy- density science and bring the nation a large step closer to developing fusion energy for civilian use. The NIF PSA includes evaluations of the potential environmental impacts of constructing and operating the facility at one of five candidate site and for two design options.

Transcriptional profiling of nitrogen fixation and the role of NifA in the diazotrophic endophyte Azoarcus sp. strain BH72.

Directory of Open Access Journals (Sweden)

Abhijit Sarkar

Full Text Available BACKGROUND: The model endophyte Azoarcus sp. strain BH72 is known to contribute fixed nitrogen to its host Kallar grass and also expresses nitrogenase genes endophytically in rice seedlings. Availability of nitrogen is a signal regulating the transcription of nitrogenase genes. Therefore, we analysed global transcription in response to differences in the nitrogen source. METHODOLOGY/PRINCIPAL FINDINGS: A DNA microarray, comprising 70-mer oligonucleotides representing 3989 open reading frames of the genome of strain BH72, was used for transcriptome studies. Transcription profiles of cells grown microaerobically on N2 versus ammonium were compared. Expression of 7.2% of the genes was significantly up-regulated, and 5.8% down-regulated upon N2 fixation, respectively. A parallel genome-wide prediction of σ(54-type promoter elements mapped to the upstream region of 38 sequences of which 36 were modulated under the N2 response. In addition to modulation of genes related to N2 fixation, the expressions of gene clusters that might be related to plant-microbe interaction and of several transcription factors were significantly enhanced. While comparing under N2-fixation conditions the transcriptome of wild type with a nifLA(- insertion mutant, NifA being the essential transcriptional activator for nif genes, 24.5% of the genome was found to be affected in expression. A genome-wide prediction of 29 NifA binding sequences matched to 25 of the target genes whose expression was differential during microarray analysis, some of which were putatively negatively regulated by NifA. For selected genes, differential expression was corroborated by real time RT-PCR studies. CONCLUSION/SIGNIFICANCE: Our data suggest that life under conditions of nitrogen fixation is an important part of the lifestyle of strain BH72 in roots, as a wide range of genes far beyond the nif regulon is modulated. Moreover, the NifA regulon in strain BH72 appears to encompass a wider range of

NIF Rugby High Foot Campaign from the design side

Science.gov (United States)

Leidinger, J.-P.; Callahan, D. A.; Berzak-Hopkins, L. F.; Ralph, J. E.; Amendt, P.; Hinkel, D. E.; Michel, P.; Moody, J. D.; Ross, J. S.; Rygg, J. R.; Celliers, P.; Clouët, J.-F.; Dewald, E. L.; Kaiser, P.; Khan, S.; Kritcher, A. L.; Liberatore, S.; Marion, D.; Masson-Laborde, P.-E.; Milovich, J. L.; Morice, O.; Pak, A. E.; Poujade, O.; Strozzi, D.; Hurricane, O. A.

2016-05-01

The NIF Rugby High Foot campaign results, with 8 shots to date, are compared with the 2D FCI2 design simulations. A special emphasis is placed on the predictive features and on those areas where some work is still required to achieve the best possible modelling of these MJ-class experiments.

National Ignition Facility project acquisition plan revision 1

International Nuclear Information System (INIS)

Clobes, A.R.

1996-01-01

The purpose of this National Ignition Facility Acquisition Plan is to describe the overall procurement strategy planned for the National Ignition Facility M Project. It was prepared for the NIP Prood Office by the NIF Procurement Manager

Dilation x-ray imager a new/faster gated x-ray imager for the NIF [DIXI (Dilation x-ray imager) a new/faster gated x-ray imager for the NIF

Energy Technology Data Exchange (ETDEWEB)

Nagel, S. R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hilsabeck, T. J.; Bell, P. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Bradley, D. K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ayers, M. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Barrios, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Felker, B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Smith, R. F. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Collins, G. W. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Jones, O. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kilkenny, J. D. [General Atomics, San Diego, CA (United States); Chung, T. [General Atomics, San Diego, CA (United States); Piston, K. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Raman, K. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Sammuli, B. [General Atomics, San Diego, CA (United States); Hares, J. D. [Kentech Instruments Ltd., Wallingford, Oxfordshire (United Kingdom); Dymoke-Bradshaw, A. K. L. [Kentech Instruments Ltd., Wallingford, Oxfordshire (United Kingdom)

2012-07-19

As the yield on implosion shots increases it is expected that the peak x-ray emission reduces to a duration with a FWHM as short as 20 ps for ~7 10¹⁸ neutron yield. However, the temporal resolution of currently used gated x-ray imagers on the NIF is 40-100 ps. We discuss the benefits of the higher temporal resolution for the NIF and present performance measurements for DIXI, which utilizes pulse-dilation technology [1] to achieve x-ray imaging with temporal gate times below 10 ps. Lastly, the measurements were conducted using the COMET laser, which is part of the Jupiter Laser Facility at the Lawrence Livermore National Laboratory.

The NIF DISCO Framework: facilitating automated integration of neuroscience content on the web.

Science.gov (United States)

Marenco, Luis; Wang, Rixin; Shepherd, Gordon M; Miller, Perry L

2010-06-01

This paper describes the capabilities of DISCO, an extensible approach that supports integrative Web-based information dissemination. DISCO is a component of the Neuroscience Information Framework (NIF), an NIH Neuroscience Blueprint initiative that facilitates integrated access to diverse neuroscience resources via the Internet. DISCO facilitates the automated maintenance of several distinct capabilities using a collection of files 1) that are maintained locally by the developers of participating neuroscience resources and 2) that are "harvested" on a regular basis by a central DISCO server. This approach allows central NIF capabilities to be updated as each resource's content changes over time. DISCO currently supports the following capabilities: 1) resource descriptions, 2) "LinkOut" to a resource's data items from NCBI Entrez resources such as PubMed, 3) Web-based interoperation with a resource, 4) sharing a resource's lexicon and ontology, 5) sharing a resource's database schema, and 6) participation by the resource in neuroscience-related RSS news dissemination. The developers of a resource are free to choose which DISCO capabilities their resource will participate in. Although DISCO is used by NIF to facilitate neuroscience data integration, its capabilities have general applicability to other areas of research.

Designing the owner's nuclear project management organization

International Nuclear Information System (INIS)

Cooke, T.C.; Peck, B.H.

1976-01-01

Few decisions are more important to an electric utility company than the one to build a nuclear generating facility. This decision will require continuous management attention to the nuclear project for periods of up to ten years on the part of the utility. Effective management of such a large, complex project requires an owner's organization skilled in such areas as engineering, heavy construction, procurement, and project control. The paper describes a method for designing the owner's nuclear organization. Factors considered include the identification of milestone events and phases of the project and identification of key organizational groups and their degree of involvement. A series of important decision milestones is also identified for structuring the organization. A step-by-step analysis involving a set of evaluation criteria results in a recommended organization that can be staffed by the owner according to the degree of involvement desired. This technique of analysis could also be performed using different evaluation criteria resulting in other options for the owner's organization

Charged-particle spectroscopy for diagnosing shock ρR and strength in NIF implosions.

Science.gov (United States)

Zylstra, A B; Frenje, J A; Séguin, F H; Rosenberg, M J; Rinderknecht, H G; Johnson, M Gatu; Casey, D T; Sinenian, N; Manuel, M J-E; Waugh, C J; Sio, H W; Li, C K; Petrasso, R D; Friedrich, S; Knittel, K; Bionta, R; McKernan, M; Callahan, D; Collins, G W; Dewald, E; Döppner, T; Edwards, M J; Glenzer, S; Hicks, D G; Landen, O L; London, R; Mackinnon, A; Meezan, N; Prasad, R R; Ralph, J; Richardson, M; Rygg, J R; Sepke, S; Weber, S; Zacharias, R; Moses, E; Kilkenny, J; Nikroo, A; Sangster, T C; Glebov, V; Stoeckl, C; Olson, R; Leeper, R J; Kline, J; Kyrala, G; Wilson, D

2012-10-01

The compact Wedge Range Filter (WRF) proton spectrometer was developed for OMEGA and transferred to the National Ignition Facility (NIF) as a National Ignition Campaign diagnostic. The WRF measures the spectrum of protons from D-(3)He reactions in tuning-campaign implosions containing D and (3)He gas; in this work we report on the first proton spectroscopy measurement on the NIF using WRFs. The energy downshift of the 14.7-MeV proton is directly related to the total ρR through the plasma stopping power. Additionally, the shock proton yield is measured, which is a metric of the final merged shock strength.

The magnetic recoil spectrometer for measurements of the absolute neutron spectrum at OMEGA and the NIF.

Science.gov (United States)

Casey, D T; Frenje, J A; Johnson, M Gatu; Séguin, F H; Li, C K; Petrasso, R D; Glebov, V Yu; Katz, J; Magoon, J; Meyerhofer, D D; Sangster, T C; Shoup, M; Ulreich, J; Ashabranner, R C; Bionta, R M; Carpenter, A C; Felker, B; Khater, H Y; LePape, S; MacKinnon, A; McKernan, M A; Moran, M; Rygg, J R; Yeoman, M F; Zacharias, R; Leeper, R J; Fletcher, K; Farrell, M; Jasion, D; Kilkenny, J; Paguio, R

2013-04-01

The neutron spectrum produced by deuterium-tritium (DT) inertial confinement fusion implosions contains a wealth of information about implosion performance including the DT yield, ion-temperature, and areal-density. The Magnetic Recoil Spectrometer (MRS) has been used at both the OMEGA laser facility and the National Ignition Facility (NIF) to measure the absolute neutron spectrum from 3 to 30 MeV at OMEGA and 3 to 36 MeV at the NIF. These measurements have been used to diagnose the performance of cryogenic target implosions to unprecedented accuracy. Interpretation of MRS data requires a detailed understanding of the MRS response and background. This paper describes ab initio characterization of the system involving Monte Carlo simulations of the MRS response in addition to the commission experiments for in situ calibration of the systems on OMEGA and the NIF.

The magnetic recoil spectrometer for measurements of the absolute neutron spectrum at OMEGA and the NIF

International Nuclear Information System (INIS)

Casey, D. T.; Frenje, J. A.; Gatu Johnson, M.; Séguin, F. H.; Li, C. K.; Petrasso, R. D.; Glebov, V. Yu.; Katz, J.; Magoon, J.; Meyerhofer, D. D.; Sangster, T. C.; Shoup, M.; Ulreich, J.; Ashabranner, R. C.; Bionta, R. M.; Carpenter, A. C.; Felker, B.; Khater, H. Y.; LePape, S.; MacKinnon, A.

2013-01-01

The neutron spectrum produced by deuterium-tritium (DT) inertial confinement fusion implosions contains a wealth of information about implosion performance including the DT yield, ion-temperature, and areal-density. The Magnetic Recoil Spectrometer (MRS) has been used at both the OMEGA laser facility and the National Ignition Facility (NIF) to measure the absolute neutron spectrum from 3 to 30 MeV at OMEGA and 3 to 36 MeV at the NIF. These measurements have been used to diagnose the performance of cryogenic target implosions to unprecedented accuracy. Interpretation of MRS data requires a detailed understanding of the MRS response and background. This paper describes ab initio characterization of the system involving Monte Carlo simulations of the MRS response in addition to the commission experiments for in situ calibration of the systems on OMEGA and the NIF.

Perspectives: Using Historical Documents To Think about NIF Issues.

Science.gov (United States)

National Archives and Records Service (GSA), Washington, DC.

The purpose of using historical documents in the classroom is to generate and enhance discussion by providing a historical perspective for issues. Five documents are included in this packet and are to be used as a supplemental material for the National Issues Forum (NIF) topics. Issues raised include (1) an analysis of the documents and (2)…

Analysis of NIF experiments with the minimal energy implosion model

Energy Technology Data Exchange (ETDEWEB)

Cheng, B., E-mail: bcheng@lanl.gov; Kwan, T. J. T.; Wang, Y. M.; Merrill, F. E.; Batha, S. H. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Cerjan, C. J. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States)

2015-08-15

We apply a recently developed analytical model of implosion and thermonuclear burn to fusion capsule experiments performed at the National Ignition Facility that used low-foot and high-foot laser pulse formats. Our theoretical predictions are consistent with the experimental data. Our studies, together with neutron image analysis, reveal that the adiabats of the cold fuel in both low-foot and high-foot experiments are similar. That is, the cold deuterium-tritium shells in those experiments are all in a high adiabat state at the time of peak implosion velocity. The major difference between low-foot and high-foot capsule experiments is the growth of the shock-induced instabilities developed at the material interfaces which lead to fuel mixing with ablator material. Furthermore, we have compared the NIF capsules performance with the ignition criteria and analyzed the alpha particle heating in the NIF experiments. Our analysis shows that alpha heating was appreciable only in the high-foot experiments.

Analysis of NIF experiments with the minimal energy implosion model

International Nuclear Information System (INIS)

Cheng, B.; Kwan, T. J. T.; Wang, Y. M.; Merrill, F. E.; Batha, S. H.; Cerjan, C. J.

2015-01-01

We apply a recently developed analytical model of implosion and thermonuclear burn to fusion capsule experiments performed at the National Ignition Facility that used low-foot and high-foot laser pulse formats. Our theoretical predictions are consistent with the experimental data. Our studies, together with neutron image analysis, reveal that the adiabats of the cold fuel in both low-foot and high-foot experiments are similar. That is, the cold deuterium-tritium shells in those experiments are all in a high adiabat state at the time of peak implosion velocity. The major difference between low-foot and high-foot capsule experiments is the growth of the shock-induced instabilities developed at the material interfaces which lead to fuel mixing with ablator material. Furthermore, we have compared the NIF capsules performance with the ignition criteria and analyzed the alpha particle heating in the NIF experiments. Our analysis shows that alpha heating was appreciable only in the high-foot experiments

Successful healthcare programs and projects: organization portfolio management essentials.

Science.gov (United States)

Pickens, Scott; Solak, Jamie

2005-01-01

Many healthcare organization projects take more time and resources than planned and fail to deliver desired business outcomes. Healthcare IT is a major component of many projects and often undeservedly receives the blame for failure. Poor results are often not a result of faulty healthcare IT or poor project management or poor project execution alone. Many projects fail because of poor portfolio management--poor planning and management of the portfolio of initiatives designed to meet an organization's strategic goals. Because resources are limited, portfolio management enables organizations to more strategically allocate and manage their resources so care delivery, service delivery, and initiatives that advance organizations toward their strategic goals, including healthcare IT initiatives, can be accomplished at the levels of quality and service desired by an organization. Proper portfolio management is the essential foundation for program and project success and supports overall organization success. Without portfolio management, even programs and projects that execute flawlessly may not meet desired objectives. This article discusses the essential requirements for porfolio management. These include opportunity identification, return on investment (ROI) forecast, project prioritization, capacity planning (inclusive of human, financial, capital, and facilities resources), work scheduling, program and project management and execution, and project performance and value assessment. Portfolio management is essential to successful healthcare project execution. Theories are drawn from the Organizational Project Management Maturity Model (OPM3) work of the Project Management Institute and other leading strategy, planning, and organization change management research institutes.

February 2017 - NIF Highlights

Energy Technology Data Exchange (ETDEWEB)

Fournier, K. B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-03-13

February was a very productive month with only 20 shot days on the calendar. There were 41 target shots performed for the HED, ICF, and the Discovery Science (DS) program. The DS program had a week dedicated to their experiments that was extraordinarily fruitful: 14 target shots were performed for five independent teams, each of whom had a unique experimental platform to field. The teams and the facility worked extraordinarily well to pull off this feat! Additionally, the facility developed high-energy laser operations on a demonstration quad to investigate taking NIF to a new level of performance, and the ICF program demonstrated a 40% increase in the yield from a capsule that had a new, 5-μm-diameter fill tube that apparently mitigates some of the issues that have affected implosions to date. Details follow below.

Enhanced NIF neutron activation diagnostics.

Science.gov (United States)

Yeamans, C B; Bleuel, D L; Bernstein, L A

2012-10-01

The NIF neutron activation diagnostic suite relies on removable activation samples, leading to operational inefficiencies and a fundamental lower limit on the half-life of the activated product that can be observed. A neutron diagnostic system measuring activation of permanently installed samples could remove these limitations and significantly enhance overall neutron diagnostic capabilities. The physics and engineering aspects of two proposed systems are considered: one measuring the (89)Zr/(89 m)Zr isomer ratio in the existing Zr activation medium and the other using potassium zirconate as the activation medium. Both proposed systems could improve the signal-to-noise ratio of the current system by at least a factor of 5 and would allow independent measurement of fusion core velocity and fuel areal density.

Analysis of hohlraum energetics of the SG series and the NIF experiments with energy balance model

Directory of Open Access Journals (Sweden)

Guoli Ren

2017-01-01

Full Text Available The basic energy balance model is applied to analyze the hohlraum energetics data from the Shenguang (SG series laser facilities and the National Ignition Facility (NIF experiments published in the past few years. The analysis shows that the overall hohlraum energetics data are in agreement with the energy balance model within 20% deviation. The 20% deviation might be caused by the diversity in hohlraum parameters, such as material, laser pulse, gas filling density, etc. In addition, the NIF's ignition target designs and our ignition target designs given by simulations are also in accordance with the energy balance model. This work confirms the value of the energy balance model for ignition target design and experimental data assessment, and demonstrates that the NIF energy is enough to achieve ignition if a 1D spherical radiation drive could be created, meanwhile both the laser plasma instabilities and hydrodynamic instabilities could be suppressed.

A Platform for X-Ray Thomson Scattering Measurements of Radiation Hydrodynamics Experiments on the NIF

Science.gov (United States)

Lefevre, Heath; Ma, Kevin; Belancourt, Patrick; MacDonald, Michael; Doeppner, Tilo; Keiter, Paul; Kuranz, Carolyn

2017-10-01

A recent experiment on the National Ignition Facility (NIF) radiographed the evolution of the Rayleigh-Taylor (RT) instability under high and low drive cases. This experiment showed that under a high drive the growth rate of the RT instability is reduced relative to the low drive case. The high drive launches a radiative shock, increases the temperature of the post-shock region, and ablates the spikes, which reduces the RT growth rate. The plasma parameters must be measured to validate this claim. We present a target design for making X-Ray Thomson Scattering (XRTS) measurements on radiation hydrodynamics experiments on NIF to measure the electron temperature of the shocked region in the above cases. Specifically, we show that a previously fielded NIF radiation hydrodynamics platform can be modified to allow sufficient signal and temperature resolution for XRTS measurements. This work is funded by the NNSA-DS and SC-OFES Joint Program in High-Energy-Density Laboratory Plasmas, Grant Number DE-NA0002956 and the National Science Foundation through the Basic Plasma Science and Engineering program.

Polarimetry of uncoupled light on the NIF.

Science.gov (United States)

Turnbull, D; Moody, J D; Michel, P; Ralph, J E; Divol, L

2014-11-01

Polarimetry has been added to the full aperture backscatter diagnostic on the NIF. Wollaston prisms are used to sample a small region of a beam's backscatter, effectively separating it into two linear polarizations, one of which is parallel to the incident beam. A time-averaged measurement of each polarization is obtained by imaging the separated spots off of a scatter plate. Results have improved understanding of crossed beam energy transfer, glint, and sidescatter, and motivated plans to upgrade to a time-resolved polarimeter measuring the full Stokes vector.

Polarimetry of uncoupled light on the NIF

Energy Technology Data Exchange (ETDEWEB)

Turnbull, D., E-mail: turnbull2@llnl.gov; Moody, J. D.; Michel, P.; Ralph, J. E.; Divol, L. [National Ignition Facility and Photon Science, Lawrence Livermore National Laboratory, Livermore, California 94550 (United States)

2014-11-15

Polarimetry has been added to the full aperture backscatter diagnostic on the NIF. Wollaston prisms are used to sample a small region of a beam's backscatter, effectively separating it into two linear polarizations, one of which is parallel to the incident beam. A time-averaged measurement of each polarization is obtained by imaging the separated spots off of a scatter plate. Results have improved understanding of crossed beam energy transfer, glint, and sidescatter, and motivated plans to upgrade to a time-resolved polarimeter measuring the full Stokes vector.

Nitrogenase (nifH gene expression in diazotrophic cyanobacteria in the Tropical North Atlantic in response to nutrient amendments

Directory of Open Access Journals (Sweden)

Kendra A Turk-Kubo

2012-11-01

Full Text Available The Tropical North Atlantic (TNAtl plays a critical role in the marine nitrogen cycle, as it supports high rates of biological nitrogen (N2 fixation, yet it is unclear whether this process is limited by the availability of iron (Fe, phosphate (P or is co-limited by both. In order to investigate the impact of nutrient limitation on the N2-fixing microorganisms (diazotrophs in the TNAtl, trace metal clean nutrient amendment experiments were conducted, and the expression of nitrogenase (nifH in cyanobacterial diazotrophs in response to the addition of Fe, P, or Fe+P was measured using quantitative PCR. To provide context, N2 fixation rates associated with the <10 μm community and diel nifH expression in natural cyanobacterial populations were measured. In the western TNAtl, nifH expression in Crocosphaera, Trichodesmium, and Richelia was stimulated by Fe and Fe+P additions, but not by P, implying that diazotrophs may be Fe-limited in this region. In the eastern TNAtl, nifH expression in unicellular cyanobacteria UCYN-A and Crocosphaera was stimulated by P, implying P-limitation. In equatorial waters, nifH expression in Trichodesmium was highest in Fe+P treatments, implying co-limitation in this region. Nutrient additions did not measurably stimulate N2 fixation rates in the <10 μm fraction in most of the experiments, even when upregulation of nifH expression was evident. These results demonstrate the utility of using gene expression to investigate the physiological state of natural populations of microorganisms, while underscoring the complexity of nutrient limitation on diazotrophy, and providing evidence that diazotroph populations are slow to respond to the addition of limiting nutrients and may be limited by different nutrients on basin-wide spatial scales. This has important implications for our current understanding of controls on N2 fixation in the TNAtl and may partially explain why it appears to be intermittently limited by Fe, P, or

OMEGA ICF experiments and preparations for direct drive on NIF

International Nuclear Information System (INIS)

McCrory, R.L.; Bahr, R.E.; Betti, R.

2001-01-01

Direct-drive laser-fusion ignition experiments rely on detailed understanding and control of irradiation uniformity, the Rayleigh-Taylor instability, and target fabrication. LLE is investigating various theoretical aspects of a direct-drive NIF ignition target based on an 'all-DT' design: a spherical target of ∼3.4-mm diameter, 1 to 2 μm of CH wall thickness, and an ∼340-μm DT-ice layer near the triple point of DT (∼19 K). OMEGA experiments are designed to address the critical issues related to direct-drive laser fusion and to provide the necessary data to validate the predictive capability of LLE computer codes. The cryogenic targets to be used on OMEGA are hydrodynamically equivalent to those planned for the NIF. The current experimental studies on OMEGA address the essential components of direct-drive laser fusion: irradiation uniformity and laser imprinting, Rayleigh-Taylor growth and saturation, compressed core performance and shell fuel mixing, laser plasma interactions and their effect on target performance, and cryogenic target fabrication and handling. (author)

Data Analysis of the Gated-LEH X-Ray Imaging Diagnostic at the NIF

Science.gov (United States)

Thibodeau, Matthew; Chen, Hui

2017-10-01

The Gated Laser Entrance Hole (G-LEH) x-ray imaging diagnostic in use at the NIF offers a desirable combination of spatial and temporal resolution. By looking inside of NIF hohlraums with time resolution, G-LEH measures target features including LEH size and capsule size. A framework is presented for automated and systematic analysis of G-LEH images that measures several physical parameters of interest and their evolution over time. The results from these analyses enable comparisons with hohlraum models and allow model validation of LEH closure velocity and the extent of capsule blow-off. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1.

Science.gov (United States)

Garapaty, Shivani; Mahajan, Muktar A; Samuels, Herbert H

2008-03-14

CCR4-NOT is an evolutionarily conserved, multicomponent complex known to be involved in transcription as well as mRNA degradation. Various subunits (e.g. CNOT1 and CNOT7/CAF1) have been reported to be involved in influencing nuclear hormone receptor activities. Here, we show that CCR4/CNOT6 and RCD1/CNOT9, members of the CCR4-NOT complex, potentiate nuclear receptor activity. RCD1 interacts in vivo and in vitro with NIF-1 (NRC-interacting factor), a previously characterized nuclear receptor cotransducer that activates nuclear receptors via its interaction with NRC. As with NIF-1, RCD1 and CCR4 do not directly associate with nuclear receptors; however, they enhance ligand-dependent transcriptional activation by nuclear hormone receptors. CCR4 mediates its effect through the ligand binding domain of nuclear receptors and small interference RNA-mediated silencing of endogenous CCR4 results in a marked decrease in nuclear receptor activation. Furthermore, knockdown of CCR4 results in an attenuated stimulation of RARalpha target genes (e.g. Sox9 and HoxA1) as shown by quantitative PCR assays. The silencing of endogenous NIF-1 also resulted in a comparable decrease in the RAR-mediated induction of both Sox9 and HoxA1. Furthermore, CCR4 associates in vivo with NIF-1. In addition, the CCR4-enhanced transcriptional activation by nuclear receptors is dependent on NIF-1. The small interference RNA-mediated knockdown of NIF-1 blocks the ligand-dependent potentiating effect of CCR4. Our results suggest that CCR4 plays a role in the regulation of certain endogenous RARalpha target genes and that RCD1 and CCR4 might mediate their function through their interaction with NIF-1.

Testing a new NIF neutron time-of-flight detector with a bibenzyl scintillator on OMEGA.

Science.gov (United States)

Glebov, V Yu; Forrest, C; Knauer, J P; Pruyne, A; Romanofsky, M; Sangster, T C; Shoup, M J; Stoeckl, C; Caggiano, J A; Carman, M L; Clancy, T J; Hatarik, R; McNaney, J; Zaitseva, N P

2012-10-01

A new neutron time-of-flight (nTOF) detector with a bibenzyl crystal as a scintillator has been designed and manufactured for the National Ignition Facility (NIF). This detector will replace a nTOF20-Spec detector with an oxygenated xylene scintillator currently operational on the NIF to improve the areal-density measurements. In addition to areal density, the bibenzyl detector will measure the D-D and D-T neutron yield and the ion temperature of indirect- and direct-drive-implosion experiments. The design of the bibenzyl detector and results of tests on the OMEGA Laser System are presented.

Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense

International Nuclear Information System (INIS)

Sotomaior, P.; Araújo, L.M.; Nishikawa, C.Y.; Huergo, L.F.; Monteiro, R.A.; Pedrosa, F.O.; Chubatsu, L.S.; Souza, E.M.

2012-01-01

Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate

Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense.

Science.gov (United States)

Sotomaior, P; Araújo, L M; Nishikawa, C Y; Huergo, L F; Monteiro, R A; Pedrosa, F O; Chubatsu, L S; Souza, E M

2012-12-01

Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate.

Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense

Energy Technology Data Exchange (ETDEWEB)

Sotomaior, P.; Araújo, L.M.; Nishikawa, C.Y.; Huergo, L.F.; Monteiro, R.A.; Pedrosa, F.O.; Chubatsu, L.S.; Souza, E.M. [Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR (Brazil)

2012-09-21

Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate.

Multiple levels in the organisation of innovation : project organization in single-firm projects and multi-firm projects

NARCIS (Netherlands)

Jaspers, F.P.H.; Ende, van den J.C.M.; Borgh, van der W.; Yin, Jie

2008-01-01

Studies about how the organization of new product (and new service) development projects (NPD) projects influences project performance typically investigate this in Single-firm projects, i.e. projects with high ownership integration. However, NPD projects are often performed by two or more

Neutron peak velocity measurements at the National Ignition Facility (NIF) using novel quartz detectors

Science.gov (United States)

Grim, Gary; Eckart, Mark; Hartouni, Edward; Hatarik, Robert; Moore, Alastair; Root, Jaben; Sayre, Daniel; Schlossberg, David; Waltz, Cory

2017-10-01

In mid-2017 the NIF implemented quartz based neutron time-of-flight (nToF) detectors which have a faster and narrower impulse response function (IRF) relative to traditional scintillator detectors. In this presentation we report on comparisons between fusion neutron first moments as measured by quartz and scintillator based detectors using DT layered implosions at the NIF. We report on the change in precision presaged by the quartz converter and quantify the change in both in shot, line-of-site velocity variability. as well as, shot-to-shot variation. Work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344. LLNL-ABS-734511-DRAFT.

p35 regulates the CRM1-dependent nucleocytoplasmic shuttling of nuclear hormone receptor coregulator-interacting factor 1 (NIF-1.

Directory of Open Access Journals (Sweden)

Xiao-Su Zhao

Full Text Available Cyclin-dependent kinase 5 (Cdk5 is a proline-directed serine/threonine kinase, which plays critical roles in a wide spectrum of neuronal functions including neuronal survival, neurite outgrowth, and synapse development and plasticity. Cdk5 activity is controlled by its specific activators: p35 or p39. While knockout studies reveal that Cdk5/p35 is critical for neuronal migration during early brain development, functions of Cdk5/p35 have been unraveled through the identification of the interacting proteins of p35, most of which are Cdk5/p35 substrates. However, it remains unclear whether p35 can regulate neuronal functions independent of Cdk5 activity. Here, we report that a nuclear protein, nuclear hormone receptor coregulator (NRC-interacting factor 1 (NIF-1, is a new interacting partner of p35. Interestingly, p35 regulates the functions of NIF-1 independent of Cdk5 activity. NIF-1 was initially discovered as a transcriptional regulator that enhances the transcriptional activity of nuclear hormone receptors. Our results show that p35 interacts with NIF-1 and regulates its nucleocytoplasmic trafficking via the nuclear export pathway. Furthermore, we identified a nuclear export signal on p35; mutation of this site or blockade of the CRM1/exportin-dependent nuclear export pathway resulted in the nuclear accumulation of p35. Intriguingly, blocking the nuclear export of p35 attenuated the nuclear accumulation of NIF-1. These findings reveal a new p35-dependent mechanism in transcriptional regulation that involves the nucleocytoplasmic shuttling of transcription regulators.

p35 regulates the CRM1-dependent nucleocytoplasmic shuttling of nuclear hormone receptor coregulator-interacting factor 1 (NIF-1).

Science.gov (United States)

Zhao, Xiao-Su; Fu, Wing-Yu; Chien, Winnie W Y; Li, Zhen; Fu, Amy K Y; Ip, Nancy Y

2014-01-01

Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine kinase, which plays critical roles in a wide spectrum of neuronal functions including neuronal survival, neurite outgrowth, and synapse development and plasticity. Cdk5 activity is controlled by its specific activators: p35 or p39. While knockout studies reveal that Cdk5/p35 is critical for neuronal migration during early brain development, functions of Cdk5/p35 have been unraveled through the identification of the interacting proteins of p35, most of which are Cdk5/p35 substrates. However, it remains unclear whether p35 can regulate neuronal functions independent of Cdk5 activity. Here, we report that a nuclear protein, nuclear hormone receptor coregulator (NRC)-interacting factor 1 (NIF-1), is a new interacting partner of p35. Interestingly, p35 regulates the functions of NIF-1 independent of Cdk5 activity. NIF-1 was initially discovered as a transcriptional regulator that enhances the transcriptional activity of nuclear hormone receptors. Our results show that p35 interacts with NIF-1 and regulates its nucleocytoplasmic trafficking via the nuclear export pathway. Furthermore, we identified a nuclear export signal on p35; mutation of this site or blockade of the CRM1/exportin-dependent nuclear export pathway resulted in the nuclear accumulation of p35. Intriguingly, blocking the nuclear export of p35 attenuated the nuclear accumulation of NIF-1. These findings reveal a new p35-dependent mechanism in transcriptional regulation that involves the nucleocytoplasmic shuttling of transcription regulators.

Performance of the NIF prototype beamlet

International Nuclear Information System (INIS)

Van Wonterghem, B.M.; Murray, J.R.; Speck, D.R.; Campbell, J.H.

1994-01-01

Beamlet is a full scale single beam prototype laser system, built to demonstrate the laser technology and performance of the 192 beam National Ignition Facility (NIF) fusion laser driver. Both laser systems apply multipass amplifier architectures. By passing the beam four times through the large aperture amplifier sections, the small signal gain during the first few passes is used efficiently to reduce expensive staged amplifier chains. The beamlet prototype laser integrates results of development programs for large aperture components: large aperture optical switch, polarizers, 2 x 2 multisegment amplifiers and new pulse generation and pre-amplification techniques. The authors report on performance test results of the recently completed 1 ω-laser section of Beamlet

Cherenkov radiation conversion and collection considerations for a gamma bang time/reaction history diagnostic for the NIF.

Science.gov (United States)

Herrmann, Hans W; Mack, Joseph M; Young, Carlton S; Malone, Robert M; Stoeffl, Wolfgang; Horsfield, Colin J

2008-10-01

Bang time and reaction history measurements are fundamental components of diagnosing inertial confinement fusion (ICF) implosions and will be essential contributors to diagnosing attempts at ignition on the National Ignition Facility (NIF). Fusion gammas provide a direct measure of fusion interaction rate without being compromised by Doppler spreading. Gamma-based gas Cherenkov detectors that convert fusion gamma rays to optical Cherenkov photons for collection by fast recording systems have been developed and fielded at Omega. These systems have established their usefulness in illuminating ICF physics in several experimental campaigns. Bang time precision better than 25 ps has been demonstrated, well below the 50 ps accuracy requirement defined by the NIF system design requirements. A comprehensive, validated numerical study of candidate systems is providing essential information needed to make a down selection based on optimization of sensitivity, bandwidth, dynamic range, cost, and NIF logistics. This paper presents basic design considerations arising from the two-step conversion process from gamma rays to relativistic electrons to UV/visible Cherenkov radiation.

Cherenkov radiation conversion and collection considerations for a gamma bang time/reaction history diagnostic for the NIF

International Nuclear Information System (INIS)

Herrmann, Hans W.; Mack, Joseph M.; Young, Carlton S.; Malone, Robert M.; Stoeffl, Wolfgang; Horsfield, Colin J.

2008-01-01

Bang time and reaction history measurements are fundamental components of diagnosing inertial confinement fusion (ICF) implosions and will be essential contributors to diagnosing attempts at ignition on the National Ignition Facility (NIF). Fusion gammas provide a direct measure of fusion interaction rate without being compromised by Doppler spreading. Gamma-based gas Cherenkov detectors that convert fusion gamma rays to optical Cherenkov photons for collection by fast recording systems have been developed and fielded at Omega. These systems have established their usefulness in illuminating ICF physics in several experimental campaigns. Bang time precision better than 25 ps has been demonstrated, well below the 50 ps accuracy requirement defined by the NIF system design requirements. A comprehensive, validated numerical study of candidate systems is providing essential information needed to make a down selection based on optimization of sensitivity, bandwidth, dynamic range, cost, and NIF logistics. This paper presents basic design considerations arising from the two-step conversion process from γ rays to relativistic electrons to UV/visible Cherenkov radiation.

Backscatter measurements for NIF ignition targets (invited).

Science.gov (United States)

Moody, J D; Datte, P; Krauter, K; Bond, E; Michel, P A; Glenzer, S H; Divol, L; Niemann, C; Suter, L; Meezan, N; MacGowan, B J; Hibbard, R; London, R; Kilkenny, J; Wallace, R; Kline, J L; Knittel, K; Frieders, G; Golick, B; Ross, G; Widmann, K; Jackson, J; Vernon, S; Clancy, T

2010-10-01

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of ∼15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Backscatter measurements for NIF ignition targets (invited)

Energy Technology Data Exchange (ETDEWEB)

Moody, J. D.; Datte, P.; Krauter, K.; Bond, E.; Michel, P. A.; Glenzer, S. H.; Divol, L.; Suter, L.; Meezan, N.; MacGowan, B. J.; Hibbard, R.; London, R.; Kilkenny, J.; Wallace, R.; Knittel, K.; Frieders, G.; Golick, B.; Ross, G.; Widmann, K.; Jackson, J. [Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, California 94550 (United States); and others

2010-10-15

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of {approx}15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Backscatter measurements for NIF ignition targets (invited)

International Nuclear Information System (INIS)

Moody, J. D.; Datte, P.; Krauter, K.; Bond, E.; Michel, P. A.; Glenzer, S. H.; Divol, L.; Suter, L.; Meezan, N.; MacGowan, B. J.; Hibbard, R.; London, R.; Kilkenny, J.; Wallace, R.; Knittel, K.; Frieders, G.; Golick, B.; Ross, G.; Widmann, K.; Jackson, J.

2010-01-01

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of ∼15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Stability analysis of directly driven Nif capsules

Energy Technology Data Exchange (ETDEWEB)

Goncharov, V.N.; Skupsky, S.; McKenty, P.W.; Delettrez, J.A.; Town, R.P.J. [Rochester Univ., NY (United States). Lab. for Laser Energetics; Cherfils-Clerouin, C. [CEA/DAM-Ile de France, DIF, 91 - Bruyeres Le Chatel (France)

2000-07-01

An analytical model is presented to study perturbation evolution at the ablation and inner surfaces of the imploding shell. The model describes the ablative Rayleigh-Taylor and Bell-Plesset instabilities. The initial conditions for the model are determined by using existing theories of laser imprint, ablative Richtmyer-Meshkov instability, 'feed-out' and by performing a series of 2-D ORCHID simulations. The model and simulations show that the direct-drive cryogenic {alpha} = 3 NIF capsules remain intact during the implosion if laser nonuniformities are smoothed by 2-D SSD used in the current direct-drive target designs. (authors)

DIM and diagnostic placement for NIF experiments

International Nuclear Information System (INIS)

Kalantar, D.

1999-01-01

The input that has been provided on the NIF experiment setup sheets has allowed us to review the diagnostic and DIM placement as well as the baseline unconverted light management plan. We have done an iteration to identify common diagnostic lines of sight, and with additional requirements defined by specific experiments, we propose (1) a baseline plan for DIM placement requiring only five DIMs that may be moved between up to seven DIM ports, and (2) a modified baseline unconverted light management plan. We request additional input to identify primary vs. secondary diagnostics for each experiment definition

First Results from the South Pole Bang Time (SPBT) Diagnostic on the NIF*

Science.gov (United States)

Edgell, D. H.; Glebov, V. Yu.; Magoon, J.; Sangster, T. C.; Shoup, M. J., III; Stoeckl, C.; Macphee, A.; Bradley, D. K.; Burns, S.; Celeste, J.; Eckart, M. J.; Jones, O. S.; Kilkenny, J. D.; Kimbrough, J. R.; MacKinnon, A. J.; Parker, J.; Thomas, T.

2011-10-01

The south pole bang time (SPBT) x-ray diagnostic has been successfully fielded on the NIF. SPBT consists of chemical-vapor-deposition diamond detectors, with different filtrations, located 3 m directly below target chamber center, viewing the implosion through the hohlraum laser entrance hole. The diamond detectors are sensitive to both x rays and neutrons. HOPG crystal mirror monochromators increase the x-ray signal to background ratio. SPBT is designed to measure the x-ray bang time with an accuracy of a few tens of picoseconds. SPBT x-ray and neutron results from NIF implosions are presented along with timing and error analysis. *This work was supported by the U.S. Department of Energy Office of Inertial Confinement Fusion under Cooperative Agreement No. DE-FC52-08NA28302.

X-ray flux and X-ray burn through experiments on reduced-scale targets at the Nif and OMEGA lasers

International Nuclear Information System (INIS)

Schneider, M.B.; Hinkel, D.E.; Young, B.K.; Holder, J.P.; Langdon, A.B.; Bower, D.E.; Bruns, H.C.; Campbell, K.M.; Celeste, J.R.; Compton, S.; Costa, R.L.; Dewald, E.L.; Dixit, S.N.; Eckart, M.J.; Eder, D.C.; Edwards, M.J.; Ellis, A.D.; Emig, J.A.; Froula, D.H.; Glebov, V.; Glenzer, S.H.; Hargrove, D.; Haynam, C.A.; Heeter, R.F.; Henesian, M.A.; Holtmeier, G.; James, D.L.; Jancaitis, K.S.; Kalantar, D.H.; Kamperschroer, J.H.; Kauffman, R.L.; Kimbrough, J.; Kirkwood, R.; Koniges, A.E.; Landen, O.L.; Landon, M.; Lee, F.D.; MacGowan, B.J.; Mackinnon, A.J.; Manes, K.R.; Marshall, C.; May, M.J.; McDonald, J.W.; Menapace, J.; Moon, S.J.; Moses, E.I.; Munro, D.H.; Murray, J.R.; Niemann, C.; Piston, K.; Power, G.D.; Rekow, V.; Ruppe, J.A.; Schein, J.; Shepherd, R.; Singh, M.S.; Sorce, C.; Springer, P.T.; Still, C.H.; Suter, L.J.; Tietbohl, G.L.; Turner, R.E.; Van Wonterghem, B.M.; Wallace, R.J.; Warrick, A.; Weber, F.; Wegner, P.J.; Williams, E.A.; Young, P.E.; Baldis, H.A.; Constantin, C.G.; Bahr, R.; Roberts, S.; Seka, W.; Stoeckl, C.; Pellinen, D.; Watts, P.

2006-01-01

An experimental campaign to maximize radiation drive in small-scale hohlraums has been carried out at the National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (Livermore, USA) and at the OMEGA laser at the Laboratory for Laser Energetics (Rochester, USA). The small-scale hohlraums, laser energy, laser pulse, and diagnostics were similar at both facilities but the geometries were very different. The NIF experiments used on-axis laser beams whereas the OMEGA experiments used 19 beams in three beam cones. In the cases when the lasers coupled well and produced similar radiation drive, images of X-ray burn-through and laser deposition indicate the pattern of plasma filling is very different. The OMEGA targets fill faster than the NIF targets, which helps explain the time behavior of the X-ray fluences. (authors)

X-ray flux and X-ray burn through experiments on reduced-scale targets at the Nif and OMEGA lasers

Energy Technology Data Exchange (ETDEWEB)

Schneider, M.B.; Hinkel, D.E.; Young, B.K.; Holder, J.P.; Langdon, A.B.; Bower, D.E.; Bruns, H.C.; Campbell, K.M.; Celeste, J.R.; Compton, S.; Costa, R.L.; Dewald, E.L.; Dixit, S.N.; Eckart, M.J.; Eder, D.C.; Edwards, M.J.; Ellis, A.D.; Emig, J.A.; Froula, D.H.; Glebov, V.; Glenzer, S.H.; Hargrove, D.; Haynam, C.A.; Heeter, R.F.; Henesian, M.A.; Holtmeier, G.; James, D.L.; Jancaitis, K.S.; Kalantar, D.H.; Kamperschroer, J.H.; Kauffman, R.L.; Kimbrough, J.; Kirkwood, R.; Koniges, A.E.; Landen, O.L.; Landon, M.; Lee, F.D.; MacGowan, B.J.; Mackinnon, A.J.; Manes, K.R.; Marshall, C.; May, M.J.; McDonald, J.W.; Menapace, J.; Moon, S.J.; Moses, E.I.; Munro, D.H.; Murray, J.R.; Niemann, C.; Piston, K.; Power, G.D.; Rekow, V.; Ruppe, J.A.; Schein, J.; Shepherd, R.; Singh, M.S.; Sorce, C.; Springer, P.T.; Still, C.H.; Suter, L.J.; Tietbohl, G.L.; Turner, R.E.; Van Wonterghem, B.M.; Wallace, R.J.; Warrick, A.; Weber, F.; Wegner, P.J.; Williams, E.A.; Young, P.E. [Lawrence Livermore National Lab., Livermore, CA (United States); Baldis, H.A.; Constantin, C.G. [California at Davis Univ., CA (United States); Bahr, R.; Roberts, S.; Seka, W.; Stoeckl, C. [Laboratory for Laser Energetics, Rochester, NY (United States); Pellinen, D.; Watts, P. [Bechtel Nevada Corporation, Livermore, CA (United States)

2006-06-15

An experimental campaign to maximize radiation drive in small-scale hohlraums has been carried out at the National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (Livermore, USA) and at the OMEGA laser at the Laboratory for Laser Energetics (Rochester, USA). The small-scale hohlraums, laser energy, laser pulse, and diagnostics were similar at both facilities but the geometries were very different. The NIF experiments used on-axis laser beams whereas the OMEGA experiments used 19 beams in three beam cones. In the cases when the lasers coupled well and produced similar radiation drive, images of X-ray burn-through and laser deposition indicate the pattern of plasma filling is very different. The OMEGA targets fill faster than the NIF targets, which helps explain the time behavior of the X-ray fluences. (authors)

Precision Neutron Time-of-Flight Detectors Provide Insight into NIF Implosion Dynamics

Science.gov (United States)

Schlossberg, David; Eckart, M. J.; Grim, G. P.; Hartouni, E. P.; Hatarik, R.; Moore, A. S.; Waltz, C. S.

2017-10-01

During inertial confinement fusion, higher-order moments of neutron time-of-flight (nToF) spectra can provide essential information for optimizing implosions. The nToF diagnostic suite at the National Ignition Facility (NIF) was recently upgraded to include novel, quartz Cherenkov detectors. These detectors exploit the rapid Cherenkov radiation process, in contrast with conventional scintillator decay times, to provide high temporal-precision measurements that support higher-order moment analyses. Preliminary measurements have been made on the NIF during several implosions and initial results are presented here. Measured line-of-sight asymmetries, for example in ion temperatures, will be discussed. Finally, advanced detector optimization is shown to advance accessible physics, with possibilities for energy discrimination, gamma source identification, and further reduction in quartz response times. Work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344.

Organizational Enablers for Project Governance and Governmentality in Project-based Organizations

DEFF Research Database (Denmark)

Pemsel, Sofia; Müller, Ralf; Shao, Jingting

for governmentality belong mainly to the cultural-cognitive pillar. Collectively these OEs provide for an ambidexterity of flexibility and stability, which allows organizations to align their internal characteristics with their organizational context. Managerial and theoretical implications of the results......This study identifies organizational enablers (OEs) for governance and governmentality in the realm of projects in project-based organizations (PBOs). We use a multiple case study design with six firms in Sweden and China to identify OEs. Institutional theory serves as theoretical perspective...

Organizational Enablers for Project Governance and Governmentality in Project-based Organizations

DEFF Research Database (Denmark)

Müller, Ralf; Pemsel, Sofia; Shao, Jingting

2015-01-01

for governmentality belong mainly to the cultural–cognitive pillar. Collectively these OEs provide for an ambidexterity of flexibility and stability, which allows organizations to align their internal characteristics with their organizational context. Managerial and theoretical implications of the results......This study identifies organizational enablers (OEs) for governance and governmentality in the realm of projects in project-based organizations (PBOs). We use a multiple case study design with six firms in Sweden and China to identify OEs. Institutional theory serves as theoretical perspective...

Dilation x-ray imager a new∕faster gated x-ray imager for the NIF.

Science.gov (United States)

Nagel, S R; Hilsabeck, T J; Bell, P M; Bradley, D K; Ayers, M J; Barrios, M A; Felker, B; Smith, R F; Collins, G W; Jones, O S; Kilkenny, J D; Chung, T; Piston, K; Raman, K S; Sammuli, B; Hares, J D; Dymoke-Bradshaw, A K L

2012-10-01

As the yield on implosion shots increases it is expected that the peak x-ray emission reduces to a duration with a FWHM as short as 20 ps for ∼7 × 10(18) neutron yield. However, the temporal resolution of currently used gated x-ray imagers on the NIF is 40-100 ps. We discuss the benefits of the higher temporal resolution for the NIF and present performance measurements for dilation x-ray imager, which utilizes pulse-dilation technology [T. J. Hilsabeck et al., Rev. Sci. Instrum. 81, 10E317 (2010)] to achieve x-ray imaging with temporal gate times below 10 ps. The measurements were conducted using the COMET laser, which is part of the Jupiter Laser Facility at the Lawrence Livermore National Laboratory.

The involvement of the nif-associated ferredoxin-like genes fdxA and fdxN of Herbaspirillum seropedicae in nitrogen fixation.

Science.gov (United States)

Souza, André L F; Invitti, Adriana L; Rego, Fabiane G M; Monteiro, Rose A; Klassen, Giseli; Souza, Emanuel M; Chubatsu, Leda S; Pedrosa, Fábio O; Rigo, Liu U

2010-02-01

The pathway of electron transport to nitrogenase in the endophytic beta-Proteobacterium Herbaspirillum seropedicae has not been characterized. We have generated mutants in two nif-associated genes encoding putative ferredoxins, fdxA and fdxN. The fdxA gene is part of the operon nifHDKENXorf1orf2fdxAnifQmodABC and is transcribed from the nifH promoter, as revealed by lacZ gene fusion. The fdxN gene is probably cotranscribed with the nifB gene. Mutational analysis suggests that the FdxA protein is essential for maximum nitrogenase activity, since the nitrogenase activity of the fdxA mutant strain was reduced to about 30% of that of the wild-type strain. In addition, the fdxA mutation had no effect on the nitrogenase switch-off in response to ammonium. Nitrogenase activity of a mutant strain lacking the fdxN gene was completely abolished. This phenotype was reverted by complementation with fdxN expressed under lacZ promoter control. The results suggest that the products of both the fdxA and fdxN genes are probably involved in electron transfer during nitrogen fixation.

Management of Multi-projects In a Process oriented Organization

OpenAIRE

Macheridis, Nikos; Nilsson, Carl-Henrik

2006-01-01

When projects are used as an organisational platform to conduct business, a project can be the only project in the organisation or one amongst several others. The latter case is called multi-project organisation. Usually an organization with a multi-project environment has a base organisation, which can be functional, matrix structure or another. The purpose of this article is to develop a model based on a process oriented organization as a complement to functional or matrix organizational st...

A trial to combine heterogeneous computer systems in NIFS

International Nuclear Information System (INIS)

Emoto, M.; Watanabe, K.; Ohdachi, S.; Matsunami, S.; Yamaguchi, S.; Sudo, S.; Okumura, H.

2000-01-01

Several computer systems in NIFS (the National Institute for Fusion Science) are involved in the operation of the LHD (Large Helical Device) for plasma experiments. Because these systems are independent of each other, it is burdensome to the programmers and the researchers to use several of them for one project. Currently, the programmers must write low-level data access routines for each system, then the researchers, after having learned these routines, can install the applications to retrieve the data on each system. In order to improve this situation, we have been developing a new system. This system employs two technologies, the signed applet and HORB, to combine the independent systems. These technologies were chosen for the following reasons: (1) The signed applet is an applet free from the general restrictions of applets, and can connect to any server and save the retrieved data to client machines; and (2) HORB is the Java-based ORB (Object Request Broker), the use of which makes it easy to build a Java-based, distributed environment. When the system is completed, it will allow users to obtain data from the above independent systems by means of ordinary Web browsers, without having to install additional applications

Progress Toward Modeling Spectroscopic Signatures of Mix on Omega and NIF

Science.gov (United States)

Tregillis, I. L.; Schmitt, M. J.; Hsu, S. C.; Wysocki, F. J.; Cobble, J. A.; Murphy, T. J.

2011-10-01

Defect-induced mix processes may degrade the performance of ICF and ICF-like targets at Omega and NIF. An improved understanding of the relevant physics requires an experimental program built on a foundation of radiation-hydrodynamic simulations plus reliable synthetic diagnostic outputs. To that end, the Applications of Ignition (AoI) and Defect Implosion Experiment (DIME) efforts at LANL have focused on directly driven plastic capsules containing high-Z dopants and manufactured with an equatorial ``trench'' defect. One of the key diagnostic techniques for detecting and diagnosing the migration of dopant material into the hot core is Multi-Monochromatic X-ray Imaging (MMI). This talk will focus on recent efforts to model spectroscopic signatures of mix processes in AoI/DIME capsules via simulated MMI-type diagnostic instruments. It will also include data from recent Omega shots and calculations in support of Tier 1 experiments at NIF in FY2012. This work is supported by US DOE/NNSA, performed at LANL, operated by LANS LLC under contract DE-AC52-06NA25396.

Precision Cleaning and Protection of Coated Optical Components for NIF Small Optics

Energy Technology Data Exchange (ETDEWEB)

Phelps, Jim [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-10-19

The purpose of this procedure shall be to define the precision cleaning of finished, coated, small optical components for NIF at Lawrence Livermore National Laboratories. The term “small optical components” includes coated optics that are set into simple mounts, as well as coated, un-mounted optics.

Final report for NIF chamber dynamics studies

International Nuclear Information System (INIS)

Burnham, A; Peterson, P F; Scott, J M

1998-01-01

The National Ignition Facility (NIF), a 1.8 MJ, 192 laser beam facility, will have anticipated fusion yields of up to 20 MJ from D-T pellets encased in a gold hohlraum target. The energy emitted from the target in the form of x rays, neutrons, target debris kinetic energy, and target shrapnel will be contained in a 5 m. radius spherical target chamber. various diagnostics will be stationed around the target at varying distances from the target. During each shot, the target will emit x rays that will vaporize nearby target facing surfaces including those of the diagnostics, the target positioner, and other chamber structures. This ablated vapor will be transported throughout the chamber, and will eventually condense and deposit on surfaces in the chamber, including the final optics debris shields. The research at the University of California at Berkeley relates primarily to the NIF chamber dynamics. The key design issues are the ablation of the chamber structures, transport of the vapor through the chamber and the condensation or deposition processes of those vaporized materials. An understanding of these processes is essential in developing a concept for protecting the fina optics debris shields from an excessive coating (> 10 A) of target debris and ablated material, thereby prolonging their lifetime between change-outs. At Berkeley, we have studied the physical issues of the ablation process and the effects of varying materials, the condensation process of the vaporized material, and design schemes that can lower the threat posed to the debris shields by these processes. The work or portions of the work completed this year have been published in several papers and a dissertation [l-5

Status Update: Modeling Energy Balance in NIF Hohlraums

Energy Technology Data Exchange (ETDEWEB)

Jones, O. S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-07-22

We have developed a standardized methodology to model hohlraum drive in NIF experiments. We compare simulation results to experiments by 1) comparing hohlraum xray fluxes and 2) comparing capsule metrics, such as bang times. Long-pulse, high gas-fill hohlraums require a 20-28% reduction in simulated drive and inclusion of ~15% backscatter to match experiment through (1) and (2). Short-pulse, low fill or near-vacuum hohlraums require a 10% reduction in simulated drive to match experiment through (2); no reduction through (1). Ongoing work focuses on physical model modifications to improve these matches.

Differential accumulation of nif structural gene mRNA in Azotobacter vinelandii.

Science.gov (United States)

Hamilton, Trinity L; Jacobson, Marty; Ludwig, Marcus; Boyd, Eric S; Bryant, Donald A; Dean, Dennis R; Peters, John W

2011-09-01

Northern analysis was employed to investigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif structural genes and in the intergenic noncoding regions. The results indicate that intergenic RNA secondary structures effect the differential accumulation of transcripts, supporting the high Fe protein-to-MoFe protein ratio required for optimal diazotrophic growth.

AXIS: An instrument for imaging Compton radiographs using the Advanced Radiography Capability on the NIF

Energy Technology Data Exchange (ETDEWEB)

Hall, G. N., E-mail: hall98@llnl.gov; Izumi, N.; Tommasini, R.; Carpenter, A. C.; Palmer, N. E.; Zacharias, R.; Felker, B.; Holder, J. P.; Allen, F. V.; Bell, P. M.; Bradley, D.; Montesanti, R.; Landen, O. L. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550 (United States)

2014-11-15

Compton radiography is an important diagnostic for Inertial Confinement Fusion (ICF), as it provides a means to measure the density and asymmetries of the DT fuel in an ICF capsule near the time of peak compression. The AXIS instrument (ARC (Advanced Radiography Capability) X-ray Imaging System) is a gated detector in development for the National Ignition Facility (NIF), and will initially be capable of recording two Compton radiographs during a single NIF shot. The principal reason for the development of AXIS is the requirement for significantly improved detection quantum efficiency (DQE) at high x-ray energies. AXIS will be the detector for Compton radiography driven by the ARC laser, which will be used to produce Bremsstrahlung X-ray backlighter sources over the range of 50 keV–200 keV for this purpose. It is expected that AXIS will be capable of recording these high-energy x-rays with a DQE several times greater than other X-ray cameras at NIF, as well as providing a much larger field of view of the imploded capsule. AXIS will therefore provide an image with larger signal-to-noise that will allow the density and distribution of the compressed DT fuel to be measured with significantly greater accuracy as ICF experiments are tuned for ignition.

AXIS: an instrument for imaging Compton radiographs using the Advanced Radiography Capability on the NIF.

Science.gov (United States)

Hall, G N; Izumi, N; Tommasini, R; Carpenter, A C; Palmer, N E; Zacharias, R; Felker, B; Holder, J P; Allen, F V; Bell, P M; Bradley, D; Montesanti, R; Landen, O L

2014-11-01

Compton radiography is an important diagnostic for Inertial Confinement Fusion (ICF), as it provides a means to measure the density and asymmetries of the DT fuel in an ICF capsule near the time of peak compression. The AXIS instrument (ARC (Advanced Radiography Capability) X-ray Imaging System) is a gated detector in development for the National Ignition Facility (NIF), and will initially be capable of recording two Compton radiographs during a single NIF shot. The principal reason for the development of AXIS is the requirement for significantly improved detection quantum efficiency (DQE) at high x-ray energies. AXIS will be the detector for Compton radiography driven by the ARC laser, which will be used to produce Bremsstrahlung X-ray backlighter sources over the range of 50 keV-200 keV for this purpose. It is expected that AXIS will be capable of recording these high-energy x-rays with a DQE several times greater than other X-ray cameras at NIF, as well as providing a much larger field of view of the imploded capsule. AXIS will therefore provide an image with larger signal-to-noise that will allow the density and distribution of the compressed DT fuel to be measured with significantly greater accuracy as ICF experiments are tuned for ignition.

National Ignition Facility site requirements

International Nuclear Information System (INIS)

1996-07-01

The Site Requirements (SR) provide bases for identification of candidate host sites for the National Ignition Facility (NIF) and for the generation of data regarding potential actual locations for the facilities. The SR supplements the NIF Functional Requirements (FR) with information needed for preparation of responses to queries for input to HQ DOE site evaluation. The queries are to include both documents and explicit requirements for the potential host site responses. The Sr includes information extracted from the NIF FR (for convenience), data based on design approaches, and needs for physical and organization infrastructure for a fully operational NIF. The FR and SR describe requirements that may require new construction or may be met by use or modification of existing facilities. The SR do not establish requirements for NIF design or construction project planning. The SR document does not constitute an element of the NIF technical baseline

Low fuel convergence path to ignition on the NIF

Science.gov (United States)

Schmitt, M. J.; Molvig, Kim; Gianakon, T. A.; Woods, C. N.; Krasheninnikova, N. S.; Hsu, S. C.; Schmidt, D. W.; Dodd, E. S.; Zylstra, Alex; Scheiner, B.; McKenty, P.; Campbell, E. M.; Froula, D.; Betti, R.; Michel, T.

2017-10-01

A novel concept for achieving ignition on the NIF is proposed that obviates current issues plaguing single-shell high-convergence capsules. A large directly-driven Be shell is designed to robustly implode two nested internal shells by efficiently converting 1.7MJ of laser energy from a 6 ns, low intensity laser pulse, into a 1 ns dynamic pressure pulse to ignite and burn a central liquid DT core after a fuel convergence of only 9. The short, low intensity laser pulse mitigates LPI allowing more uniform laser drive of the target and eliminates hot e-, preheat and laser zooming issues. Preliminary rad-hydro simulations predict ignition initiation with 90% maximum inner shell velocity, before deceleration Rayleigh-Taylor growth can cause significant pusher shell mix into the compressed DT fuel. The gold inner pusher shell reduces pre-ignition radiation losses from the fuel allowing ignition to occur at 2.5keV. Further 2D simulations show that the short pulse design results in a spatially uniform kinetic drive that is tolerant to variations in laser cone power. A multi-pronged effort, in collaboration with LLE, is progressing to optimize this design for NIF's PDD laser configuration. Work performed under the auspices of the U.S. Dept. of Energy by the Los Alamos National Security, LLC, Los Alamos National Laboratory under contract DE-FG02-051ER54810.

Dilation x-ray imager a new/faster gated x-ray imager for the NIF

Energy Technology Data Exchange (ETDEWEB)

Nagel, S. R.; Bell, P. M.; Bradley, D. K.; Ayers, M. J.; Barrios, M. A.; Felker, B.; Smith, R. F.; Collins, G. W.; Jones, O. S.; Piston, K.; Raman, K. S. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550 (United States); Hilsabeck, T. J.; Kilkenny, J. D.; Chung, T.; Sammuli, B. [General Atomics, P.O. Box 85608, San Diego, California 92186-5608 (United States); Hares, J. D.; Dymoke-Bradshaw, A. K. L. [Kentech Instruments Ltd., Wallingford, Oxfordshire OX10 (United Kingdom)

2012-10-15

As the yield on implosion shots increases it is expected that the peak x-ray emission reduces to a duration with a FWHM as short as 20 ps for {approx}7 Multiplication-Sign 10{sup 18} neutron yield. However, the temporal resolution of currently used gated x-ray imagers on the NIF is 40-100 ps. We discuss the benefits of the higher temporal resolution for the NIF and present performance measurements for dilation x-ray imager, which utilizes pulse-dilation technology [T. J. Hilsabeck et al., Rev. Sci. Instrum. 81, 10E317 (2010)] to achieve x-ray imaging with temporal gate times below 10 ps. The measurements were conducted using the COMET laser, which is part of the Jupiter Laser Facility at the Lawrence Livermore National Laboratory.

Molecular phylogeny, population genetics, and evolution of heterocystous cyanobacteria using nifH gene sequences

Czech Academy of Sciences Publication Activity Database

Singh, P.; Singh, S. S.; Elster, Josef; Mishra, A. K.

2013-01-01

Roč. 250, č. 3 (2013), s. 751-764 ISSN 0033-183X Institutional support: RVO:67985939 Keywords : evolution * heterocystous cyanobacteria * nifH gene Subject RIV: EH - Ecology, Behaviour Impact factor: 3.171, year: 2013

Group Organized Project Work in Distance Education

DEFF Research Database (Denmark)

Helbo, Jan; Knudsen, Morten; Jensen, Lars Peter

2001-01-01

Project organized problem based learning is a successful concept for on-campus education at Aalborg University. Recently this "Aalborg concept" has been used in networked distance education as well. This paper describes the experiences from two years of Internet-mediated project work in a new...... Master of Information Technology education. The main conclusions are, that the project work is a strong learning motivator, enhancing peer collaboration, for off-campus students as well. However, the concept cannot be directly transferred to off-campus learning. The main reasons are that the students...... must communicate electronically, and that they are under a fierce time strain, studying part time and typically with a full time job and a family. In this paper, the main problems experienced with group organized project work in distance education are described, and some possible solutions are listed...

Project organized Problem-based learning in Distance Education

DEFF Research Database (Denmark)

Jensen, Lars Peter; Helbo, Jan; Knudsen, Morten

2002-01-01

Project organized problem based learning is a successful concept for on-campus engineering education at Aalborg University. Recently this "Aalborg concept" has been used in networked distance education as well. This paper describes the experiences from two years of Internet-mediated project work...... in a new Master of Information Technology education. The main conclusions are, that the project work is a strong learning motivator, enhancing peer collaboration, for off-campus students as well. However, the concept cannot be directly transferred to off-campus learning. In this paper, the main problems...... experienced with group organized project work in distance education are described, and some possible solutions are listed....

Project-Organized Problem-Based Learning in Distance Education

DEFF Research Database (Denmark)

Jensen, Lars Peter; Helbo, Jan; Knudsen, Morten

2003-01-01

Project organized problem based learning is a successful concept for on-campus engineering education at Aalborg University. Recently this "Aalborg concept" has been used in networked distance education as well. This paper describes the experiences from two years of Internet-mediated project work...... in a new Master of Information Technology education. The main conclusions are, that the project work is a strong learning motivator, enhancing peer collaboration, for off-campus students as well. However, the concept cannot be directly transferred to off-campus learning. In this paper, the main problems...... experienced with group organized project work in distance education are described, and some possible solutions are listed....

An assessment of the 3D geometric surrogacy of shock timing diagnostic techniques for tuning experiments on the NIF

Energy Technology Data Exchange (ETDEWEB)

Robey, H F; Munro, D H; Spears, B K; Marinak, M M; Jones, O S; Patel, M V; Haan, S W; Salmonson, J D; Landen, O L [Lawrence Livermore National Laboratory, Livermore, CA (United States); Boehly, T R [Laboratory for Laser Energetics, Rochester, NY (United States); Nikroo, A [General Atomics, San Diego, CA (United States)], E-mail: robey1@llnl.gov

2008-05-15

Ignition capsule implosions planned for the National Ignition Facility (NIF) require a pulse shape with a carefully designed series of four steps, which launch a corresponding series of shocks through the ablator and DT ice shell. The relative timing of these shocks is critical for maintaining the DT fuel on a low adiabat. The current NIF specification requires that the timing of all four shocks be tuned to an accuracy of {<=} +/- 100ps. To meet these stringent requirements, dedicated tuning experiments are being planned to measure and adjust the shock timing on NIF. These tuning experiments will be performed in a modified hohlraum geometry, where a re-entrant Au cone is added to the standard NIF hohlraum to provide optical diagnostic (VISAR and SOP) access to the shocks as they break out of the ablator. This modified geometry is referred to as the 'keyhole' hohlraum and introduces a geometric difference between these tuning-experiments and the full ignition geometry. In order to assess the surrogacy of this modified geometry, 3D simulations using HYDRA [1] have been performed. The results from simulations of a quarter of the target geometry are presented. Comparisons of the hohlraum drive conditions and the resulting effect on the shock timing in the keyhole hohlraum are compared with the corresponding results for the standard ignition hohlraum.

An assessment of the 3D geometric surrogacy of shock timing diagnostic techniques for tuning experiments on the NIF

International Nuclear Information System (INIS)

Robey, H F; Munro, D H; Spears, B K; Marinak, M M; Jones, O S; Patel, M V; Haan, S W; Salmonson, J D; Landen, O L; Boehly, T R; Nikroo, A

2008-01-01

Ignition capsule implosions planned for the National Ignition Facility (NIF) require a pulse shape with a carefully designed series of four steps, which launch a corresponding series of shocks through the ablator and DT ice shell. The relative timing of these shocks is critical for maintaining the DT fuel on a low adiabat. The current NIF specification requires that the timing of all four shocks be tuned to an accuracy of ≤ +/- 100ps. To meet these stringent requirements, dedicated tuning experiments are being planned to measure and adjust the shock timing on NIF. These tuning experiments will be performed in a modified hohlraum geometry, where a re-entrant Au cone is added to the standard NIF hohlraum to provide optical diagnostic (VISAR and SOP) access to the shocks as they break out of the ablator. This modified geometry is referred to as the 'keyhole' hohlraum and introduces a geometric difference between these tuning-experiments and the full ignition geometry. In order to assess the surrogacy of this modified geometry, 3D simulations using HYDRA [1] have been performed. The results from simulations of a quarter of the target geometry are presented. Comparisons of the hohlraum drive conditions and the resulting effect on the shock timing in the keyhole hohlraum are compared with the corresponding results for the standard ignition hohlraum

On the design of the NIF Continuum Spectrometer

Science.gov (United States)

Thorn, D. B.; MacPhee, A.; Ayers, J.; Galbraith, J.; Hardy, C. M.; Izumi, N.; Bradley, D. K.; Pickworth, L. A.; Bachmann, B.; Kozioziemski, B.; Landen, O.; Clark, D.; Schneider, M. B.; Hill, K. W.; Bitter, M.; Nagel, S.; Bell, P. M.; Person, S.; Khater, H. Y.; Smith, C.; Kilkenny, J.

2017-08-01

In inertial confinement fusion (ICF) experiments on the National Ignition Facility (NIF), measurements of average ion temperature using DT neutron time of flight broadening and of DD neutrons do not show the same apparent temperature. Some of this may be due to time and space dependent temperature profiles in the imploding capsule which are not taken into account in the analysis. As such, we are attempting to measure the electron temperature by recording the free-free electron-ion scattering-spectrum from the tail of the Maxwellian temperature distribution. This will be accomplished with the new NIF Continuum Spectrometer (ConSpec) which spans the x-ray range of 20 keV to 30 keV (where any opacity corrections from the remaining mass of the ablator shell are negligible) and will be sensitive to temperatures between ˜ 3 keV and 6 keV. The optical design of the ConSpec is designed to be adaptable to an x-ray streak camera to record time resolved free-free electron continuum spectra for direct measurement of the dT/dt evolution across the burn width of a DT plasma. The spectrometer is a conically bent Bragg crystal in a focusing geometry that allows for the dispersion plane to be perpendicular to the spectrometer axis. Additionally, to address the spatial temperature dependence, both time integrated and time resolved pinhole and penumbral imaging will be provided along the same polar angle. The optical and mechanical design of the instrument is presented along with estimates for the dispersion, solid angle, photometric sensitivity, and performance.

Project in determination of crystal structure of nitrogen fixation proteins from azospirilum brasiliense and herbaspirilum seropedicae by synchrotron x-ray diffraction

International Nuclear Information System (INIS)

Barbosa, Valma M.; Leggs, Luciana A.; Delboni, Luis F.; Chubatsu, LedaS.; Souza, Emanuel M.; Machado, Hidevaldo B.; Yates, Geoffrey M.; Pedrosa, Fabio O.

1996-01-01

Full text. Biological nitrogen fixation is essential for maintaining the nitrogen cycle on earth and of high importance for Brazilian agriculture. The nitrogenase enzyme system, which provides the biochemical machinery for nitrogen fixation, consists of two component metalloproteins, the molybdenumiron (Mo Fe) protein and the iron (Fe) protein. Nitrogen fixation is a very energy-intensive process, requiring around 16 moles of ATP for each mol of N 2 fixed (reduced). As a consequence, synthesis and activity of nitrogenase is tighty regulated at two levels: general and specific. The general level regulation is mediated by the ntr (nitrogen regulation) system. Two gene products are involved: the ntrB gene product (NtrB) is responsible for the activation of the ntrC gene product (NtrC) by phosphorylating a conserved Asp54, which activates the expression of the nifA gene. The nif specific control system is mediated by the NifA protein, which binds to a DNA specific sequence (UAS, Upstream Activator Sequence) and activates nif promoter transcriptions by RNA polymerase- α54 , following ATP hydrolysis. The aim of this project is to solve the crystal structure of dinitrogenase reductase (iron protein) and dinitrogenase (molybdenum-iron protein) from Azospirilim brasiliense and the regulatory proteins NifA from Herbaspirillum seropedicae and NtrC Azospirillum brasiliense. The three dimensional structure of the proteins involved in this project will allow a better understanding of the mechanism of biological nitrogen fixation. To this end, the data collection will probably be done at the LNLS facilities which will be available in the near future. (author)

Project in determination of crystal structure of nitrogen fixation proteins from azospirilum brasiliense and herbaspirilum seropedicae by synchrotron x-ray diffraction

Energy Technology Data Exchange (ETDEWEB)

Barbosa, Valma M.; Leggs, Luciana A.; Delboni, Luis F.; Chubatsu, LedaS.; Souza, Emanuel M.; Machado, Hidevaldo B.; Yates, Geoffrey M.; Pedrosa, Fabio O. [Parana Univ., Curitiba, PR (Brazil). Dept. de Bioquimica

1996-12-31

Full text. Biological nitrogen fixation is essential for maintaining the nitrogen cycle on earth and of high importance for Brazilian agriculture. The nitrogenase enzyme system, which provides the biochemical machinery for nitrogen fixation, consists of two component metalloproteins, the molybdenumiron (Mo Fe) protein and the iron (Fe) protein. Nitrogen fixation is a very energy-intensive process, requiring around 16 moles of ATP for each mol of N{sub 2} fixed (reduced). As a consequence, synthesis and activity of nitrogenase is tighty regulated at two levels: general and specific. The general level regulation is mediated by the ntr (nitrogen regulation) system. Two gene products are involved: the ntrB gene product (NtrB) is responsible for the activation of the ntrC gene product (NtrC) by phosphorylating a conserved Asp54, which activates the expression of the nifA gene. The nif specific control system is mediated by the NifA protein, which binds to a DNA specific sequence (UAS, Upstream Activator Sequence) and activates nif promoter transcriptions by RNA polymerase-{sup {alpha}54}, following ATP hydrolysis. The aim of this project is to solve the crystal structure of dinitrogenase reductase (iron protein) and dinitrogenase (molybdenum-iron protein) from Azospirilim brasiliense and the regulatory proteins NifA from Herbaspirillum seropedicae and NtrC Azospirillum brasiliense. The three dimensional structure of the proteins involved in this project will allow a better understanding of the mechanism of biological nitrogen fixation. To this end, the data collection will probably be done at the LNLS facilities which will be available in the near future. (author)

Science of NIF scale capsule development (activities for FY97)

International Nuclear Information System (INIS)

Hamilton, K.E.; Buckley, S.R.; Cook, R.R.

1997-01-01

The focus of this work is the production of 2-mm PαMS mandrels by microencapsulation for use as National Ignition Facility (NIF) laser targets. It is our findings thus far that the processing techniques used previously for the 0.5-mm and 1.0-mm targets are no longer useful for preparation of the larger targets for a few fundamental reasons. The driving force for sphericity (from the minimization of interracial energy) decreases as the radius of curvature increases. Simultaneously, the mechanical robustness /stability of the water-oil-water emulsion droplets decreases as the droplet size increases. The impact of these physical conditions and the possibilities of circumventing these limitations have been examined while attempting to meet the NIF shell power spectrum criteria. Identifying the key parameters in the transition (solidification) from a w-o-w droplet to a solid polymer shell has been understood implicitly to be the paramount goal. It is believed through the knowledge gained that it will be possible to minimize the deleterious forces and maximize shell sphericity. At this point it is believed that properties intrinsic to the polymer (i.e., PαMS) such as its solution behavior and evolution of film stresses control the overall shell sphericity

Long-duration planar direct-drive hydrodynamics experiments on the NIF

Science.gov (United States)

Casner, A.; Mailliet, C.; Khan, S. F.; Martinez, D.; Izumi, N.; Kalantar, D.; Di Nicola, P.; Di Nicola, J. M.; Le Bel, E.; Igumenshchev, I.; Tikhonchuk, V. T.; Remington, B. A.; Masse, L.; Smalyuk, V. A.

2018-01-01

The advent of high-power lasers facilities such as the National Ignition Facility (NIF) and the laser megajoule provide unique platforms to study the physics of turbulent mixing flows in high energy density plasmas. We report here on the commissioning of a novel planar direct-drive platform on the NIF, which allows the acceleration of targets during 30 ns. Planar plastic samples were directly irradiated by 300-450 kJ of UV laser light (351 nm) and a very good planarity of the laser drive is demonstrated. No detrimental effect of imprint is observed in the case of these thick plastic targets (300 μm), which is beneficial for future academic experiments requesting similar irradiation conditions. The long-duration direct-drive (DD) platform is thereafter harnessed to study the ablative Rayleigh-Taylor instability (RTI) in DD. The growth of two-dimensional pre-imposed perturbations is quantified through time-resolved face-on x-ray radiography and used as a benchmark for radiative hydrocode simulations. The ablative RTI is then quantified in its highly nonlinear stage starting from intentionally large 3D imprinted broadband modulations. Two generations of bubble mergers is observed for the first time in DD, as a result of the unprecedented long laser acceleration.

Science of NIF scale capsule development (activities for FY97)

Energy Technology Data Exchange (ETDEWEB)

Hamilton, K.E.; Buckley, S.R.; Cook, R.R.

1997-11-12

The focus of this work is the production of 2-mm P{alpha}MS mandrels by microencapsulation for use as National Ignition Facility (NIF) laser targets. It is our findings thus far that the processing techniques used previously for the 0.5-mm and 1.0-mm targets are no longer useful for preparation of the larger targets for a few fundamental reasons. The driving force for sphericity (from the minimization of interracial energy) decreases as the radius of curvature increases. Simultaneously, the mechanical robustness /stability of the water-oil-water emulsion droplets decreases as the droplet size increases. The impact of these physical conditions and the possibilities of circumventing these limitations have been examined while attempting to meet the NIF shell power spectrum criteria. Identifying the key parameters in the transition (solidification) from a w-o-w droplet to a solid polymer shell has been understood implicitly to be the paramount goal. It is believed through the knowledge gained that it will be possible to minimize the deleterious forces and maximize shell sphericity. At this point it is believed that properties intrinsic to the polymer (i.e., P{alpha}MS) such as its solution behavior and evolution of film stresses control the overall shell sphericity.

Review of the project risk management plan in the capital projects organization at ConocoPhillips

OpenAIRE

Meidell, Camilla

2011-01-01

Master's thesis in Risk management Project Risk Management (PRM) has in recent years become an important aspect of business organization and project management. There has always been a requirement for some risk management at COPNO. However about 3 years ago the process became much more defined and has become a requirement for the contingency used on projects to be based upon the risking process. Since risk management in projects is a requirement in the CP organization it is ...

FABRICATION OF WINDOW SADDLES FOR NIF CRYOGENIC HOHLRAUMS

International Nuclear Information System (INIS)

GIRALDEZ, E; KAAE, J.L

2003-09-01

OAK-B135 A planar diagnostic viewing port attached to the cylindrical wall of the NIF cryogenic hohlraum requires a saddle-like transition piece. While the basic design of this window saddle is straightforward, its fabrication is not, given the scale and precision of the component. They solved the problem through the use of a two segment copper mandrel to electroform the gold window saddle. The segments were micro-machined using a combination of single-point diamond turning and single point diamond milling. These processes as well as the electroplating conditions, final machining and mandrel removal are described in this paper

Organizing Cultural Projects Through Legitimising as Cultural Entrepreneurship

DEFF Research Database (Denmark)

Lindkvist, Lars; Hjorth, Daniel

2015-01-01

Purpose This paper is a study of creating organization in the case of cultural projects; cultural entrepreneurship. This includes taking advantage of opportunities and using ones social capital through networks. It is a case study of Vandalorum which is an Swedish international art- and design...... and Design Centre. Findings The case of Vandalorum shows that organizing a cultural project like Vandalorum is characterized by no well-defined starting and stopping point, but – quite typically for entrepreneurship as an organization-creation process – builds momentum and legitimacy narratively. Originality...

Modeling NIF experimental designs with adaptive mesh refinement and Lagrangian hydrodynamics

Science.gov (United States)

Koniges, A. E.; Anderson, R. W.; Wang, P.; Gunney, B. T. N.; Becker, R.; Eder, D. C.; MacGowan, B. J.; Schneider, M. B.

2006-06-01

Incorporation of adaptive mesh refinement (AMR) into Lagrangian hydrodynamics algorithms allows for the creation of a highly powerful simulation tool effective for complex target designs with three-dimensional structure. We are developing an advanced modeling tool that includes AMR and traditional arbitrary Lagrangian-Eulerian (ALE) techniques. Our goal is the accurate prediction of vaporization, disintegration and fragmentation in National Ignition Facility (NIF) experimental target elements. Although our focus is on minimizing the generation of shrapnel in target designs and protecting the optics, the general techniques are applicable to modern advanced targets that include three-dimensional effects such as those associated with capsule fill tubes. Several essential computations in ordinary radiation hydrodynamics need to be redesigned in order to allow for AMR to work well with ALE, including algorithms associated with radiation transport. Additionally, for our goal of predicting fragmentation, we include elastic/plastic flow into our computations. We discuss the integration of these effects into a new ALE-AMR simulation code. Applications of this newly developed modeling tool as well as traditional ALE simulations in two and three dimensions are applied to NIF early-light target designs.

Modeling NIF Experimental Designs with Adaptive Mesh Refinement and Lagrangian Hydrodynamics

International Nuclear Information System (INIS)

Koniges, A E; Anderson, R W; Wang, P; Gunney, B N; Becker, R; Eder, D C; MacGowan, B J

2005-01-01

Incorporation of adaptive mesh refinement (AMR) into Lagrangian hydrodynamics algorithms allows for the creation of a highly powerful simulation tool effective for complex target designs with three-dimensional structure. We are developing an advanced modeling tool that includes AMR and traditional arbitrary Lagrangian-Eulerian (ALE) techniques. Our goal is the accurate prediction of vaporization, disintegration and fragmentation in National Ignition Facility (NIF) experimental target elements. Although our focus is on minimizing the generation of shrapnel in target designs and protecting the optics, the general techniques are applicable to modern advanced targets that include three-dimensional effects such as those associated with capsule fill tubes. Several essential computations in ordinary radiation hydrodynamics need to be redesigned in order to allow for AMR to work well with ALE, including algorithms associated with radiation transport. Additionally, for our goal of predicting fragmentation, we include elastic/plastic flow into our computations. We discuss the integration of these effects into a new ALE-AMR simulation code. Applications of this newly developed modeling tool as well as traditional ALE simulations in two and three dimensions are applied to NIF early-light target designs

Modeling Nif experimental designs with adaptive mesh refinement and Lagrangian hydrodynamics

International Nuclear Information System (INIS)

Koniges, A.E.; Anderson, R.W.; Wang, P.; Gunney, B.T.N.; Becker, R.; Eder, D.C.; MacGowan, B.J.; Schneider, M.B.

2006-01-01

Incorporation of adaptive mesh refinement (AMR) into Lagrangian hydrodynamics algorithms allows for the creation of a highly powerful simulation tool effective for complex target designs with three-dimensional structure. We are developing an advanced modeling tool that includes AMR and traditional arbitrary Lagrangian-Eulerian (ALE) techniques. Our goal is the accurate prediction of vaporization, disintegration and fragmentation in National Ignition Facility (NIF) experimental target elements. Although our focus is on minimizing the generation of shrapnel in target designs and protecting the optics, the general techniques are applicable to modern advanced targets that include three-dimensional effects such as those associated with capsule fill tubes. Several essential computations in ordinary radiation hydrodynamics need to be redesigned in order to allow for AMR to work well with ALE, including algorithms associated with radiation transport. Additionally, for our goal of predicting fragmentation, we include elastic/plastic flow into our computations. We discuss the integration of these effects into a new ALE-AMR simulation code. Applications of this newly developed modeling tool as well as traditional ALE simulations in two and three dimensions are applied to NIF early-light target designs. (authors)

Money and Politics: Who Owns Democracy? NIF Report on the Issues, 2001.

Science.gov (United States)

National Issues Forums, Dayton, OH.

National Issues Forums (NIF) bring together citizens to deliberate and make choices about challenging social and political issues of the day. These forums have addressed issues such as the economy, education, health care, foreign affairs, and crime. This report is an analysis of what happened in a forum on "Money and Politics" that took…

Enhancing Ignition Probability and Fusion Yield in NIF Indirect Drive Targets with Applied Magnetic Fields

Science.gov (United States)

Perkins, L. John; Logan, B. Grant; Ho, Darwin; Zimmerman, George; Rhodes, Mark; Blackfield, Donald; Hawkins, Steven

2017-10-01

Imposed magnetic fields of tens of Tesla that increase to greater than 10 kT (100 MGauss) under capsule compression may relax conditions for ignition and propagating burn in indirect-drive ICF targets. This may allow attainment of ignition, or at least significant fusion energy yields, in presently-performing ICF targets on the National Ignition Facility that today are sub-marginal for thermonuclear burn through adverse hydrodynamic conditions at stagnation. Results of detailed 2D radiation-hydrodynamic-burn simulations applied to NIF capsule implosions with low-mode shape perturbations and residual kinetic energy loss indicate that such compressed fields may increase the probability for ignition through range reduction of fusion alpha particles, suppression of electron heat conduction and stabilization of higher-mode RT instabilities. Optimum initial applied fields are around 50 T. Off-line testing has been performed of a hohlraum coil and pulsed power supply that could be integrated on NIF; axial fields of 58T were obtained. Given the full plasma structure at capsule stagnation may be governed by 3-D resistive MHD, the formation of closed magnetic field lines might further augment ignition prospects. Experiments are now required to assess the potential of applied magnetic fields to NIF ICF ignition and burn. Work performed under auspices of U.S. DOE by LLNL under Contract DE-AC52-07NA27344.

Omega experiments and preparation for moderate-gain direct-drive experiments on Nif

International Nuclear Information System (INIS)

Mr Crory, R.L.; Bahr, R.E.; Boehly, T.R.

2000-01-01

Direct-drive laser-fusion ignition experiments rely on detailed understanding and control of irradiation uniformity, Rayleigh-Taylor instability, and target fabrication. LLE is investigating various theoretical aspects of a direct-drive NIF ignition target based on an 'all-DT' design: a spherical target of ∼ 3.5 mm diameter, 1 to 2 μm if CH wall thickness, and a ∼ 350 μm DT-ice layer near the triple point of DT (μ19K). OMEGA experiments are designed to address the critical issues related to direct-drive laser fusion and to provide the necessary data to validate the predictive capability of LLE computer codes. The future cryogenic targets used on OMEGA are hydrodynamically equivalent to those planned for the NIF. The current experimental studies on OMEGA address all of the essential components of direct-drive laser fusion: irradiation uniformity and laser imprinting, Rayleigh-Taylor growth and saturation, compressed core performance and shell-fuel mixing, laser-plasma interactions and their effect on target performance, and cryogenic target fabrication and handling. (authors)

Influence of ammonium availability on expression of nifD and amtB genes during biostimulation of a U(VI) contaminated aquifer: implications for U(VI) removal and monitoring the metabolic state of Geobacteraceae

Energy Technology Data Exchange (ETDEWEB)

Mouser, Paula J.; N' Guessan, A. Lucie; Elifantz, Hila; Holmes, Dawn E.; Williams, Kenneth H; Wilkins, Michael J.; Long, Philip E.; Lovley, Derek R.

2009-03-25

The influence of ammonium availability on bacterial community structure and the physiological status of Geobacter species during in situ bioremediation of uranium-contaminated groundwater was evaluated. Ammonium concentrations varied by 2 orders of magnitude (<4 to 400 ?M) across the study site. Analysis of 16S rRNA sequences suggested that ammonium may have been one factor influencing the community composition prior to acetate amendment with Rhodoferax species predominating over Geobacter species with higher ammonium and Dechloromonas species dominating at the site with lowest ammonium. However, once acetate was added and dissimilatory metal reduction was stimulated, Geobacter species became the predominant organisms at all locations. Rates of U(VI) reduction appeared to be more related to acetate concentrations rather than ammonium levels. In situ mRNA transcript abundance of the nitrogen fixation gene, nifD, and the ammonium transporter gene, amtB, in Geobacter species indicated that ammonium was the primary source of nitrogen during uranium reduction. The abundance of amtB was inversely correlated to ammonium levels, whereas nifD transcript levels were similar across all sites examined. These results suggest that nifD and amtB expression are closely regulated in response to ammonium availability to ensure an adequate supply of nitrogen while conserving cell resources. Thus, quantifying nifD and amtB transcript expression appears to be a useful approach for monitoring the nitrogen-related physiological status of subsurface Geobacter species. This study also emphasizes the need for more detailed analysis of geochemical and physiological interactions at the field scale in order to adequately model subsurface microbial processes during bioremediation.

Formation of a homocitrate-free iron-molybdenum cluster on NifEN: implications for the role of homocitrate in nitrogenase assembly.

Science.gov (United States)

Fay, Aaron Wolfe; Blank, Michael Aaron; Yoshizawa, Janice Mariko; Lee, Chi Chung; Wiig, Jared Andrew; Hu, Yilin; Hodgson, Keith Owen; Hedman, Britt; Ribbe, Markus Walter

2010-03-28

Molybdenum (Mo)-dependent nitrogenase is a complex metalloprotein that catalyzes the biological reduction of dinitrogen (N(2)) to ammonia (NH(3)) at the molybdenum-iron cofactor (FeMoco) site of its molybdenum-iron (MoFe) protein component. Here we report the formation of a homocitrate-free, iron-molybdenum ("FeMo") cluster on the biosynthetic scaffold of FeMoco, NifEN. Such a NifEN-associated "FeMo" cluster exhibits EPR features similar to those of the NifEN-associated, fully-complemented "FeMoco", which originate from the presence of Mo in both cluster species; however, "FeMo" cluster and "FeMoco" display different temperature-dependent changes in the line shape and the signal intensity of their respective EPR features, which reflect the impact of homocitrate on the redox properties of these clusters. XAS/EXAFS analysis reveals that the Mo centers in both "FeMo" cluster and "FeMoco" are present in a similar coordination environment, although Mo in "FeMo" cluster is more loosely coordinated as compared to that in "FeMoco" with respect to the Mo-O distances in the cluster, likely due to the absence of homocitrate that normally serves as an additional ligand for the Mo in the cluster. Subsequent biochemical investigation of the "FeMo" cluster not only facilitates the determination of the sequence of events in the mobilization of Mo and homocitrate during FeMoco maturation, but also permits the examination of the role of homocitrate in the transfer of FeMoco between NifEN and MoFe protein. Combined outcome of these studies establishes a platform for future structural analysis of the interactions between NifEN and MoFe protein, which will provide useful insights into the mechanism of cluster transfer between the two proteins.

Concept of operations for channel characterization and simulation of coaxial transmission channels at the National Ignition Facility (NIF)

Energy Technology Data Exchange (ETDEWEB)

Brown, Jr., Charles G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-03-23

The National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) executes experiments for inertial con nement fusion (ICF), world-class high energy density physics (HEDP), and critical national security missions. While the laser systems, target positioners, alignment systems, control systems, etc. enable the execution of such experiments, NIF’s utility would be greatly reduced without its suite of diagnostics. It would be e ectively “blind” to the incredible physics unleashed in its target chamber. Since NIF diagnostics are such an important part of its mission, the quality and reliability of the diagnostics, and of the data recorded from them, is crucial.

THE USE OF PROJECTS IN GENERATING CHANGE IN ORGANIZATIONS

Directory of Open Access Journals (Sweden)

A. Vacar

2015-10-01

Full Text Available The article above is part of a research conducted in order to evidence the role of leaders in generating change in organizations and to observe the methods and techniques used, knowing that projects and their management are tools for implementing change in organizations. Leaders, by their influence and not by force, can determine their team to initiate new projects, considered by specialists and practitioners also, real practices of generating change in organizations.

ORGANIZATION OF FUTURE ENGINEERS' PROJECT-BASED LEARNING WHEN STUDYING THE PROJECT MANAGEMENT METHODOLOGY

Directory of Open Access Journals (Sweden)

Halyna V. Lutsenko

2015-02-01

Full Text Available The peculiarities of modern world experience of implementation of project-based learning in engineering education have been considered. The potential role and place of projects in learning activity have been analyzed. The methodology of organization of project-based activity of engineering students when studying the project management methodology and computer systems of project management has been proposed. The requirements to documentation and actual results of students' projects have been described in detail. The requirements to computer-aided systems of project management developed by using Microsoft Project in the scope of diary scheduling and resources planning have been formulated.

Transport Simulations for Fast Ignition on NIF

Energy Technology Data Exchange (ETDEWEB)

Strozzi, D J; Tabak, M; Grote, D P; Cohen, B I; Shay, H D; Town, R J; Kemp, A J; Key, M

2009-10-26

We are designing a full hydro-scale cone-guided, indirect-drive FI coupling experiment, for NIF, with the ARC-FIDO short-pulse laser. Current rad-hydro designs with limited fuel jetting into cone tip are not yet adequate for ignition. Designs are improving. Electron beam transport simulations (implicit-PIC LSP) show: (1) Magnetic fields and smaller angular spreads increase coupling to ignition-relevant 'hot spot' (20 um radius); (2) Plastic CD (for a warm target) produces somewhat better coupling than pure D (cryogenic target) due to enhanced resistive B fields; and (3) The optimal T{sub hot} for this target is {approx} 1 MeV; coupling falls by 3x as T{sub hot} rises to 4 MeV.

Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America.

Science.gov (United States)

Haukka, K; Lindström, K; Young, J P

1998-02-01

The diversity and phylogeny of nodA and nifH genes were studied by using 52 rhizobial isolates from Acacia senegal, Prosopis chilensis, and related leguminous trees growing in Africa and Latin America. All of the strains had similar host ranges and belonged to the genera Sinorhizobium and Mesorhizobium, as previously determined by 16S rRNA gene sequence analysis. The restriction patterns and a sequence analysis of the nodA and nifH genes divided the strains into the following three distinct groups: sinorhizobia from Africa, sinorhizobia from Latin America, and mesorhizobia from both regions. In a phylogenetic tree also containing previously published sequences, the nodA genes of our rhizobia formed a branch of their own, but within the branch no correlation between symbiotic genes and host trees was apparent. Within the large group of African sinorhizobia, similar symbiotic gene types were found in different chromosomal backgrounds, suggesting that transfer of symbiotic genes has occurred across species boundaries. Most strains had plasmids, and the presence of plasmid-borne nifH was demonstrated by hybridization for some examples. The nodA and nifH genes of Sinorhizobium teranga ORS1009T grouped with the nodA and nifH genes of the other African sinorhizobia, but Sinorhizobium saheli ORS609T had a totally different nodA sequence, although it was closely related based on the 16S rRNA gene and nifH data. This might be because this S. saheli strain was originally isolated from Sesbania sp., which belongs to a different cross-nodulation group than Acacia and Prosopis spp. The factors that appear to have influenced the evolution of rhizobial symbiotic genes vary in importance at different taxonomic levels.

Knowledge Governance in Project-Based Organizations

DEFF Research Database (Denmark)

Pemsel, Sofia; Wiewiora, Anna; Müller, Ralf

This paper conceptualizes knowledge governance (KG) in project-based organizations (PBOs) and its methodological approaches for empirical investigation. Three key contributions towards a multi-faceted view of KG and an understanding of KG in PBOs are advanced. These contributions include a defini......This paper conceptualizes knowledge governance (KG) in project-based organizations (PBOs) and its methodological approaches for empirical investigation. Three key contributions towards a multi-faceted view of KG and an understanding of KG in PBOs are advanced. These contributions include...... a definition of KG in PBOs, a conceptual framework to investigate KG and a methodological framework for empirical inquiry into KG in PBO settings. Our definition highlights the contingent nature of KG processes in relation to their organizational context. The conceptual framework addresses macro- and micro...

Demonstration of high coupling efficiency to Al capsule in rugby hohlraum on NIF

Science.gov (United States)

Ping, Y.; Smalyuk, V.; Amendt, P.; Bennett, D.; Chen, H.; Dewald, E.; Goyon, C.; Graziani, F.; Johnson, S.; Khan, S.; Landen, O.; Nikroo, A.; Pino, J.; Ralph, J.; Seugling, R.; Strozzi, D.; Tipton, R.; Tommasini, R.; Wang, M.; Loomis, E.; Merritt, E.; Montgomery, D.

2017-10-01

A new design of the double-shell approach predicts a high coupling efficiency from the hohlraum to the capsule, with 700 kJ in the capsule instead of 200kJ in the conventional low-Z single-shell scheme, improving prospects of double-shell performance. A recent experiment on NIF has evaluated a first step toward this goal of energy coupling using 0.7x subscale Al capsule, Au rugby hohlraum and 1MJ drive. A shell velocity of 150 μm/ns was measured, DANTE peak temperature of 255 eV was measured, and shell kinetic energy of 36 kJ was inferred using a rocket model, all close to predictions and consistent with 330kJ of total energy coupled to the capsule. Data analysis and more results from subsequent experiments will be presented. In the next step, an additional 2x increase of total coupled energy up to 700 kJ is projected for full-scale 2-MJ drive in U Rugby hohlraum. This work was performed under DOE contract DE-AC52-07NA27344.

A novel particle time of flight diagnostic for measurements of shock- and compression-bang times in D3He and DT implosions at the NIF.

Science.gov (United States)

Rinderknecht, H G; Johnson, M Gatu; Zylstra, A B; Sinenian, N; Rosenberg, M J; Frenje, J A; Waugh, C J; Li, C K; Sèguin, F H; Petrasso, R D; Rygg, J R; Kimbrough, J R; MacPhee, A; Collins, G W; Hicks, D; Mackinnon, A; Bell, P; Bionta, R; Clancy, T; Zacharias, R; Döppner, T; Park, H S; LePape, S; Landen, O; Meezan, N; Moses, E I; Glebov, V U; Stoeckl, C; Sangster, T C; Olson, R; Kline, J; Kilkenny, J

2012-10-01

The particle-time-of-flight (pTOF) diagnostic, fielded alongside a wedge range-filter (WRF) proton spectrometer, will provide an absolute timing for the shock-burn weighted ρR measurements that will validate the modeling of implosion dynamics at the National Ignition Facility (NIF). In the first phase of the project, pTOF has recorded accurate bang times in cryogenic DT, DT exploding pusher, and D(3)He implosions using DD or DT neutrons with an accuracy better than ±70 ps. In the second phase of the project, a deflecting magnet will be incorporated into the pTOF design for simultaneous measurements of shock- and compression-bang times in D(3)He-filled surrogate implosions using D(3)He protons and DD-neutrons, respectively.

A plasma amplifier to combine multiple beams at NIF

Science.gov (United States)

Kirkwood, R. K.; Turnbull, D. P.; Chapman, T.; Wilks, S. C.; Rosen, M. D.; London, R. A.; Pickworth, L. A.; Colaitis, A.; Dunlop, W. H.; Poole, P.; Moody, J. D.; Strozzi, D. J.; Michel, P. A.; Divol, L.; Landen, O. L.; MacGowan, B. J.; Van Wonterghem, B. M.; Fournier, K. B.; Blue, B. E.

2018-05-01

Combining laser beams in a plasma is enabled by seeded stimulated Brillouin scattering which allows cross-beam energy transfer (CBET) to occur and re-distributes the energy between beams that cross with different incident angles and small differences in wavelength [Kirkwood et al. Phys. Plasmas 4, 1800 (1997)]. Indirect-drive implosions at the National Ignition Facility (NIF) [Haynam et al. Appl. Opt. 46, 3276-3303 (2007)] have controlled drive symmetry by using plasma amplifiers to transfer energy between beams [Kirkwood et al., Plasma Phys. Controlled Fusion 55, 103001 (2013); Lindl et al., Phys. Plasmas 21, 020501 (2014); and Hurricane et al. Nature 506, 343-348 (2014)]. In this work, we show that the existing models are well enough validated by experiments to allow a design of a plasma beam combiner that, once optimized, is expected to produce a pulse of light in a single beam with the energy greatly enhanced over existing sources. The scheme combines up to 61 NIF beams with 120 kJ of available energy into a single f/20 beam with a 1 ns pulse duration and a 351 nm wavelength by both resonant and off-resonance CBET. Initial experiments are also described that have already succeeded in producing a 4 kJ, 1 ns pulse in a single beam by combination of up to eight incident pump beams containing <1.1 kJ/beam, which are maintained near resonance for CBET in a plasma that is formed by 60 pre-heating beams [Kirkwood et al., Nat. Phys. 14, 80 (2018)].

NIF optics phase gradient specfication

International Nuclear Information System (INIS)

Williams, W.; Auerbach, J.; Hunt, J.; Lawson, L.; Manes, K.; Orth, C.; Sacks, R.; Trenholme, J.; Wegner, P.

1997-01-01

A root-mean-square (rms) phase gradient specification seems to allow a good connection between the NIP optics quality and focal spot requirements. Measurements on Beamlet optics individually, and as a chain, indicate they meet the assumptions necessary to use this specification, and that they have a typical rms phase gradient of ∼80 angstrom/cm. This may be sufficient for NIP to meet the proposed Stockpile Stewardship Management Program (SSMP) requirements of 80% of a high- power beam within a 200-250 micron diameter spot. Uncertainties include, especially, the scale length of the optics phase noise, the ability of the adaptive optic to correct against pump-induced distortions and optics noise, and the possibility of finding mitigation techniques against whole-beam self-focusing (e.g. a pre- correction optic). Further work is needed in these areas to better determine the NIF specifications. This memo is a written summary of a presentation on this topic given by W. Williams 24 April 1997 to NIP and LS ampersand T personnel

Sensitivity of Inferred Electron Temperature from X-ray Emission of NIF Cryogenic DT Implosions

Energy Technology Data Exchange (ETDEWEB)

Klem, Michael [Univ. of Dallas, Irving, TX (United States)

2015-05-01

The National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory seeks to achieve thermonuclear ignition through inertial confinement fusion. The accurate assessment of the performance of each implosion experiment is a crucial step. Here we report on work to derive a reliable electron temperature for the cryogenic deuteriumtritium implosions completed on the NIF using the xray signal from the Ross filter diagnostic. These Xrays are dominated by bremsstrahlung emission. By fitting the xray signal measured through each of the individual Ross filters, the source bremsstrahlung spectrum can be inferred, and an electron temperature of the implosion hot spot inferred. Currently, each filter is weighted equally in this analysis. We present work quantifying the errors with such a technique and the results from investigating the contribution of each filter to the overall accuracy of the temperature inference. Using this research, we also compare the inferred electron temperature against other measured implosion quantities to develop a more complete understanding of the hotspot physics.

Genetic diversity of nifH gene sequences in Paenibacillus azotofixans strains and soil samples analyzed by denaturing gradiënt gel electrophoresis of PCR-amplified gene fragments

NARCIS (Netherlands)

Rosado, A.S.; Duarte, G.F.; Seldin, L.; Elsas, van J.D.

1998-01-01

The diversity of dinitrogenase reductase gene (nifH) fragments in Paenibacillus azotofixans strains was investigated by using molecular methods. The partial nifH gene sequences of eight P. azotofixans strains, as well as one strain each of the close relatives Paenibacillus durum, Paenibacillus

Waste Tank Organic Safety Project organic concentration mechanisms task. FY 1994 progress report

International Nuclear Information System (INIS)

Gerber, M.A.

1994-09-01

The Pacific Northwest Laboratory (PNL), Waste Tank Organic Safety Project is conducting research to support Westinghouse Hanford Company's (WHC) Waste Tank Safety Program, sponsored by the U.S. Department of Energy's Tank Farm Project Office. The goal of PNL's program is to provide a scientific basis for analyzing organics in Hanford's underground storage tanks (USTs) and for determining whether they are at concentrations that pose a potentially unsafe condition. Part of this research is directed toward determining what organic concentrations are safe by conducting research on organic aging mechanisms and waste energetics to assess the conditions necessary to produce an uncontrolled energy release in tanks due to reactions between the organics and the nitrate and nitrate salts in the tank wastes. The objective of the Organic Concentration Mechanisms Task is to assess the degree of localized enrichment of organics to be expected in the USTs due to concentration mechanisms. This report describes the progress of research conducted in FY 1994 on two concentration mechanisms of interest to the tank safety project: (1) permeation of a separate organic liquid phase into the interstitial spaces of the tank solids during the draining of free liquid from the tanks; and (2) concentration of organics on the surfaces of the solids due to adsorption. Three experiments were conducted to investigate permeation of air and solvent into a sludge simulant that is representative of single-shell tank sludge. The permeation behavior of air and solvent into the sludge simulant can be explained by the properties of the fluid pairs (air/supernate and solvent supernate) and the sludge. One important fluid property is the interfacial tension between the supernate and either the solvent or air. In general, the greater the interfacial tension between two fluids, the more difficult it will be for the air or solvent to displace the supernate during dewatering of the sludge

Observations Of The LCROSS Impact With NIFS On The Gemini North Telescope

Science.gov (United States)

Roth, Katherine; Stephens, A. W.; Trujillo, C. A.; McDermid, R. M.; Woodward, C. E.; Walls, B. D.; Coulson, D. M.; Matulonis, A. C.; Ball, J. G.; Wooden, D. H.

2010-01-01

The Lunar CRater Observation and Sensing Satellite (LCROSS) Centaur rocket impacted a permanently shadowed crater near the south pole of the Moon at 11:31 UTC 2009 October 09. Gemini, one of several telescopes in a coordinated network observing the impact, conducted observations using NIFS to obtain 3D K-band imaging spectroscopy to detect water ice in the ejected plume of material. The spectral slope of the NIFS data can constrain the grain size and height distribution as the plume evolves, measuring the total mass and the water ice concentration in the plume. These observations provided an engineering challenge for Gemini, including the need to track non-sidereal with constantly changing track rates and guide on small bright moon craters, in order to keep the impact site within the NIFS field-of-view. High quality images taken by GMOS-N, NIRI and the acquisition camera during engineering periods at specific lunar libration and illumination were also used by the LCROSS ground based observing team to supplement slit positioning and offset plans for other ground based observatories. LCROSS mission support and engineering has resulted in improved telescope functionality for non-sidereal targets, including the ability to upload and import target ephemerides directly into the TCS, starting in semester 2010B. In this poster we present the engineering results and observing improvements which will facilitate enhanced user capabilities of the Gemini telescopes arising from the intensive LCROSS support challenge. Gemini Observatory is operated by AURA, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the NSF (United States), the STFC (United Kingdom), the NRC (Canada), CONICYT (Chile), the ARC (Australia), Ministério da Ciência e Tecnologia (Brazil), and Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina). In part this research was supported by NASA through contracts to SWRI and NSF grant AST-0706980 to the U

Analysis of the project parameters of the fixed price projects in the IT services delivery organization

Directory of Open Access Journals (Sweden)

Miroslav Kral

2012-04-01

Full Text Available The success rate of IS/IT projects is low. Thus, in response, a large variety of methods and approaches to managing IS/IT projects has been developed over the years. The purpose of this paper is to analyse the relationships between the project parameters of fixed-price projects that take place within an IT service delivery organization. There is some evidence that simply using traditional project parameters (such as project duration, costs, reserves, and so on isn't enough to increase the success of projects; rather, new approaches and methods, including our applied systems approach, should also be considered. This paper aims to highlight issues from the perspective of an IT services delivery organization from the CEE region. The output of the paper should be a contribution to the project management of IS/IT projects and to systems research, as well.

Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer.

Science.gov (United States)

Coelho, Marcia Reed Rodrigues; de Vos, Marjon; Carneiro, Newton Portilho; Marriel, Ivanildo Evódio; Paiva, Edilson; Seldin, Lucy

2008-02-01

The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha(-1)). The nifH gene was amplified directly from DNA extracted from the rhizospheres, and the PCR products cloned and sequenced. Four clone libraries were generated from the nifH fragments and 245 sequences were obtained. Most of the clones (57%) were closely related to nifH genes of uncultured bacteria. NifH clones affiliated with Azohydromonas spp., Ideonella sp., Rhizobium etli and Bradyrhizobium sp. were found in all libraries. Sequences affiliated with Delftia tsuruhatensis were found in the rhizosphere of both cultivars sown with high levels of nitrogen, while clones affiliated with Methylocystis sp. were detected only in plants sown under low levels of nitrogen. Moreover, clones affiliated with Paenibacillus durus could be found in libraries from the cultivar IS 5322-C sown either in high or low amounts of fertilizer. This study showed that the amount of nitrogen used for fertilization is the overriding determinative factor that influenced the nitrogen-fixing community structures in sorghum rhizospheres cultivated in Cerrado soil.

Vesicular glutamate transporter 2 (VGLUT2) is co-stored with PACAP in projections from the rat melanopsin-containing retinal ganglion cells

DEFF Research Database (Denmark)

Engelund, Anna Iversen; Fahrenkrug, Jan; Harrison, Adrian Paul

2010-01-01

The retinal ganglion cell layer of the eye comprises a subtype of cells characterized by their intrinsic photosensitivity and expression of melanopsin (ipRGCs). These cells regulate a variety of non-image-forming (NIF) functions such as light entrainment of circadian rhythms, acute suppression......-localized in their projections in the suprachiasmatic nucleus, the intergeniculate leaflet, and the olivary pretectal nucleus. We conclude that there is evidence to support the use of glutamate and PACAP as neurotransmitters in NIF photoperception by rat ipRGCs, and that these neurotransmitters are co-stored and probably...

Ultra-stable, diode-pumped Nd-doped glass regenerative amplifier for the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Crane, J.K.; Martinez, M.; Beach, R.J.; Mitchell, S.; Pratt, G.; Christensen, J.J.

1995-12-01

We describe a diode laser-pumped Nd:glass regenerative amplifier that amplifies temporally shaped pulses with low distortion, high pulse-to- pulse stability, and high gain. This laser amplifier is a prototype subsystem for the National Ignition Facility (NIF) laser system. 2 refs., 1 fig

Change management in information systems projects for public organizations

Directory of Open Access Journals (Sweden)

Ewa Ziemba

2015-08-01

Full Text Available It can be argued that public organizations, in order to provide the public with sufficient services in the current, highly competitive and continuously evolving environment, require changes. The changes that become necessary are often related to the implementation of information systems (IS. Moreover, when organizations are faced with changes, a change management (CM process needs to be put in place. CM theories that are currently available to practitioners and academics are often contradictory; they mostly lack empirical evidence and are supported by unchallenged hypotheses concerning the nature of the contemporary CM. The aim of this paper is, therefore, to identify critical success factors (CFSs for CM in IS projects. In order to reach this aim an explanation of changes in public organizations and the nature of CM are presented. Following this, a framework of CFSs for CM in IS projects are identified based on the literature review. The paper also examines two IS projects and uses them to demonstrate CFSs influencing CM in IS projects in Polish pubic organizations. A discussion of the research findings is provided and the paper concludes with a presentation of the study’s contributions and limitations as well as the stream of future work.

Abundance and genetic diversity of nifH gene sequences in anthropogenically affected Brazilian mangrove sediments

NARCIS (Netherlands)

Franco Dias, Armando Cavalcante; Pereira e Silva, Michele de Cassia; Cotta, Simone Raposo; Dini Andreote, Francisco; Soares, Fabio Lino; Salles, Joana Falcao; Azevedo, Joao Lucio; van Elsas, Jan Dirk; Andreote, Fernando Dini

Although mangroves represent ecosystems of global importance, the genetic diversity and abundance of functional genes that are key to their functioning scarcely have been explored. Here, we present a survey based on the nifH gene across transects of sediments of two mangrove systems located along

The Virtual Design Team: Designing Project Organizations as Engineers Design Bridges

Directory of Open Access Journals (Sweden)

Raymond E. Levitt

2012-08-01

Full Text Available This paper reports on a 20-year program of research intended to advance the theory and practice of organization design for projects from its current status as an art practiced by a handful of consultants worldwide, based on their intuition and tacit knowledge, to: (1 an “organizational engineering” craft, practiced by a new generation of organizational designers; and (2 an attractive and complementary platform for new modes of “virtual synthetic organization theory research.” The paper begins with a real-life scenario that provided the motivation for developing the Virtual Design Team (VDT, an agent-based project organizational simulation tool to help managers design the work processes and organization of project teams engaged in large, semi-routine but complex and fast-paced projects. The paper sets out the underlying philosophy, representation, reasoning, and validation of VDT, and it concludes with suggestions for future research on computational modeling for organization design to extend the frontiers of organizational micro-contingency theory and expand the range of applicability and usefulness of design tools for project organizations and supply-chain networks based on this theory.

Development of the re-emit technique for ICF foot symmetry tuning for indirect drive ignition on NIF

Science.gov (United States)

Dewald, Eduard; Milovich, Jose; Edwards, John; Thomas, Cliff; Kalantar, Dan; Meeker, Don; Jones, Ogden

2007-11-01

Tuning of the the symmetry of the hohlraum radiation drive for the first 2 ns of the ICF pulse on NIF will be assessed by the re-emit technique [1] which measures the instantaneous x-ray drive asymmetry based on soft x-ray imaging of the re-emission of a high-Z sphere surrogate capsule. We will discuss the design of re-emit foot symmetry tuning measurements planned on NIF and their surrogacy for ignition experiments, including assessing the residual radiation asymmetry of the patches required for soft x-ray imaging. We will present the tuning strategy and expected accuracies based on calculations, analytical estimates and first results from scaled experiments performed at the Omega laser facility. [1] N. Delamater, G. Magelssen, A. Hauer, Phys. Rev. E 53, 5241 (1996.)

Evaulation of B4C as an ablator material for NIF capsules. Revision 1

International Nuclear Information System (INIS)

Burnham, A.K.; Alford, C.S.; Makowiecki, D.M.; Dittrich, T.R.; Wallace, R.J.; Honea, E.C.; King, C.M.; Steinman, D.

1997-01-01

Boron carbide (B 4 C) is examined as a potential fuel container and ablator for implosion capsules on the National Ignition Facility (NIF). A capsule of pure B 4 C encasing a layer of solid DT implodes stably and ignites with anticipated NIF x-ray drives, producing 18 MJ of energy. Thin films of B 4 C were found to be resistant to oxidation and modestly transmitting in the infrared (IR), possibly enabling IR fuel characterization and enhancement for thin permeation barriers but not for full-thickness capsules. Polystyrene mandrels 0.5 mm in diameter were successfully coated with 0.15-2.0 micrometers of B 4 C. Thickness estimated from optical density agreed well with those measured by scanning electron microscopy (SEM). The B 4 C microstructure was columnar but finer than for Be made at the same conditions. B 4 C is a very strong material, with a fiber tensile strength capable of holding NIF fill pressures at room temperature, but it is also very brittle, and microscopic flaws or grain structure may limit the noncryogenic fill pressure. Argon (Ar) permeation rates were measured for a few capsules that had been further coated with 5 micrometers of plasma polymer. The B 4 C coatings tended to crack under tensile load. Some shells filled more slowly than they leaked, suggesting that the cracks open and close under opposite pressure loading. As observed earlier for Ti coatings, 0.15-micrometer layers of B 4 C had better gas retention properties than 2-micrometer layers, possibly because of fewer cracks. Permeation and fill strength issues for capsules with a full ablator thickness of B 4 C are unresolved. 21 refs., 6 figs

Simulated impact of self-generated magnetic fields in the hot-spot of NIF implosions

Science.gov (United States)

Partha, M. A.; Haan, S. W.; Koning, J.; Marinak, M. M.; Weber, C. R.; Clark, D. S.

2016-10-01

Deviations from sphericity in an imploded hot-spot result in magnetic fields generated by the Biermann battery effect. The magnetic field can reduce thermal conductivity, affect α transport, change instability growth, and cause magnetic pressure. Previous estimates of these effects have indicated that they are not of great consequence, but have suggested that they could plausibly affect NIF observables such as yield and ion temperature by 5-25%. Using the MHD capability in the Hydra code, we evaluated the impact of these processes in a post-shot model for a typical NIF implosion. Various implosion asymmetries were implemented, with the goal of surveying plausible implosion configurations to find the geometry in which the MHD effects were the most significant. Magnetic fields are estimated to approach 104 Tesla, and to affect conductivity locally by more than 50%, but global impact on observables is small in most cases. Work performed under the auspices of the U.S. D.O.E. by Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344.

Environmental conditions outweigh geographical contiguity in determining the similarity of nifH-harboring microbial communities in sediments of two disconnected marginal seas

Directory of Open Access Journals (Sweden)

Haixia Zhou

2016-07-01

Full Text Available Ecological evidence suggests that heterotrophic diazotrophs fueled by organic carbon respiration in sediments play an important role in marine nitrogen fixation. However, fundamental knowledge about the identities, abundance, diversity, biogeography and controlling environmental factors of nitrogen-fixing communities in open ocean sediments is still elusive. Surprisingly, little is known also about nitrogen-fixing communities in sediments of the more research-accessible marginal seas. Here we report on an investigation of the environmental geochemistry and putative diazotrophic microbiota in the sediments of Bohai Sea, an eutrophic marginal sea of the western Pacific Ocean. Diverse and abundant nifH gene sequences were identified and sulfate-reducing bacteria were found to be the dominant putative nitrogen-fixing microbes. Community statistical analyses suggested bottom water temperature, bottom water chlorophyll a content (or the covarying turbidity and sediment porewater Eh (or the covarying pH as the most significant environmental factors controlling the structure and spatial distribution of the putative diazotrophic communities, while sediment Hg content, sulfide content and porewater SiO32--Si content were identified as the key environmental factors correlated positively with the nifH gene abundance in Bohai Sea sediments. Comparative analyses between the Bohai Sea and the northern South China Sea identified a significant composition difference of the putative diazotrophic communities in sediments between the shallow-water (estuarine and nearshore and deep-water (offshore and deep-sea environments, and sediment porewater dissolved oxygen content, water depth and in situ temperature as the key environmental factors tentatively controlling the species composition, community structure and spatial distribution of the marginal sea sediment nifH-harboring microbiota. This confirms the ecophysiological specialization and niche differentiation

Project-matrix models of marketing organization

Directory of Open Access Journals (Sweden)

Gutić Dragutin

2009-01-01

Full Text Available Unlike theory and practice of corporation organization, in marketing organization numerous forms and contents at its disposal are not reached until this day. It can be well estimated that marketing organization today in most of our companies and in almost all its parts, noticeably gets behind corporation organization. Marketing managers have always been occupied by basic, narrow marketing activities as: sales growth, market analysis, market growth and market share, marketing research, introduction of new products, modification of products, promotion, distribution etc. They rarely found it necessary to focus a bit more to different aspects of marketing management, for example: marketing planning and marketing control, marketing organization and leading. This paper deals with aspects of project - matrix marketing organization management. Two-dimensional and more-dimensional models are presented. Among two-dimensional, these models are analyzed: Market management/products management model; Products management/management of product lifecycle phases on market model; Customers management/marketing functions management model; Demand management/marketing functions management model; Market positions management/marketing functions management model. .

Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial mat.

Science.gov (United States)

Steunou, Anne-Soisig; Jensen, Sheila I; Brecht, Eric; Becraft, Eric D; Bateson, Mary M; Kilian, Oliver; Bhaya, Devaki; Ward, David M; Peters, John W; Grossman, Arthur R; Kühl, Michael

2008-04-01

Nitrogen fixation, a prokaryotic, O2-inhibited process that reduces N2 gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night, and only declined when the mat became oxic in the morning. Nitrogenase activity was low throughout the night; however, it exhibited two peaks, a small one in the evening and a large one in the early morning, when light began to stimulate cyanobacterial photosynthetic activity, but O2 consumption by respiration still exceeded the rate of O2 evolution. Once the irradiance increased to the point at which the mat became oxic, the nitrogenase activity was strongly inhibited. Transcripts for proteins associated with energy-producing metabolisms in the cell also followed diel patterns, with fermentation-related transcripts accumulating at night, photosynthesis- and respiration-related transcripts accumulating during the day and late afternoon, respectively. These results are discussed with respect to the energetics and regulation of N2 fixation in hot spring mats and factors that can markedly influence the extent of N2 fixation over the diel cycle.

The Distinct Characteristics and Strategic Impact of Emergent Projects in Large Organizations

OpenAIRE

Chiu, Lang-Hua; Minas, Henok

2009-01-01

Despite the many researches made on emergent strategies and project portfolio management, one can hardly find studies on the link between them. It can easily be assumed that emergent strategies and market dynamics have considerable effect on the portfolio of projects in organizations and, supposedly, give rise to emergent projects. We defined emergent projects to be untypical or irregular projects for the organization which are at the borderline or even outside the mainstream of the current p...

Knowledge Governance Strategies in Project-based Organizations

DEFF Research Database (Denmark)

Pemsel, Sofia; Müller, Ralf; Söderlund, Jonas

2016-01-01

Knowledge governance (KG) aims at strategically influencing knowledge processes by implementing governance mechanisms. Little is known about whether, how, or why such strategies differ among firms. We utilize a large-scale empirical study of 20 organizations to develop a typology of KG strategies...... in project-based organizations; we then explore how these strategies emerge and affect organizational knowledge processes. Six strategies are identified: Protector, Deliverer, Polisher, Explorer, Supporter, and Analyzer. This paper posits a multi-level categorization model to facilitate comparisons among KG...... strategies. We uncover three main drivers of organizations' chosen knowledge governance strategies-namely, attitudes about humans, knowledge, and knowledge control....

Advances in NIF Shock Timing Experiments

Science.gov (United States)

Robey, Harry

2012-10-01

Experiments are underway to tune the shock timing of capsule implosions on the National Ignition Facility (NIF). These experiments use a modified cryogenic hohlraum geometry designed to precisely match the performance of ignition hohlraums. The targets employ a re-entrant Au cone to provide optical access to multiple shocks as they propagate in the liquid deuterium-filled capsule interior. The strength and timing of all four shocks is diagnosed with VISAR (Velocity Interferometer System for Any Reflector). Experiments are now routinely conducted in a mirrored keyhole geometry, which allows for simultaneous diagnosis of the shock timing at both the hohlraum pole and equator. Further modifications are being made to improve the surrogacy to ignition hohlraums by replacing the standard liquid deuterium (D2) capsule fill with a deuterium-tritium (DT) ice layer. These experiments will remove any possible surrogacy difference between D2 and DT as well as incorporate the physics of shock release from the ice layer, which is absent in current experiments. Experimental results and comparisons with numerical simulation are presented.

Organ Donation European Quality System: ODEQUS project methodology.

Science.gov (United States)

Manyalich, M; Guasch, X; Gomez, M P; Páez, G; Teixeira, L

2013-01-01

Differences in the number of organ donors among hospitals cannot be explained only by the number of intensive care unit beds used or neurologic patients treated. The figures obtained are influenced by the organizational structure of the donation process and how efficient it is. The Organ Donation European Quality System (ODEQUS) is a 3-year project (from October 2010 to September 2013) co-financed by the European Agency for Health and Consumers (EAHC20091108) which aims to define a methodology to evaluate organ procurement performance at the hospital level. ODEQUS's specific objectives are to identify quality criteria and to develop quality indicators in three types of organ donation (after brain death, after cardiac death, and living donation). Those tools will be useful for hospitals' self-assessment as well as for developing an international auditing model. A consortium has been established involving 14 associated partners from Austria, Croatia, France, Germany, Italy, Poland, Portugal, Romania, Spain, Sweden, and the United Kingdom, as well as five collaborating partners from Greece, Hungary, Malta, Slovenia, and Turkey. The project has been established in three steps: 1) Design of a survey about the use of quality tools in a wide sample of European hospitals; 2) Development of quality criteria and quality indicators by the project experts. The main fields considered have been organizational structures, clinical procedures, and outcomes; and 3) Elaboration of an evaluation system to test the quality indicators in 11 European hospitals. Two types of training have been designed and performed: one concerns the development of quality criteria and quality indicators, whereas another is focused on how to use evaluation tools. Following this methodology, the project has so far identified 131 quality criteria and developed 31 quality indicators. Currently, the quality indicators are being tested in 11 selected hospitals. Copyright © 2013 Elsevier Inc. All rights

Regulation of Development and Nitrogen Fixation in Anabaena

Energy Technology Data Exchange (ETDEWEB)

James W. Golden

2008-10-17

The regulation of development and cellular differentiation is important for all multicellular organisms. The nitrogen-fixing filamentous cyanobacterium Anabaena (also Nostoc) sp. PCC 7120 (hereafter Anabaena) provides a model of multicellular microbial development and pattern formation. Anabaena reduces N2 to ammonia in specialized terminally differentiated cells called heterocysts. A one-dimensional developmental pattern of single heterocysts regularly spaced along filaments of photosynthetic vegetative cells is established to form a multicellular organism composed of these two interdependent cell types. This multicellular growth pattern, the distinct phylogeny of cyanobacteria, and the suspected antiquity of heterocyst development make this an important model system. Our long-term goal is to understand the regulatory network required for heterocyst development and nitrogen fixation. This project is focused on two key aspects of heterocyst regulation: one, the mechanism by which HetR controls the initiation of differentiation, and two, the cis and trans acting factors required for expression of the nitrogen-fixation (nif) genes. HetR is thought to be a central regulator of heterocyst development but the partners and mechanisms involved in this regulation are unknown. Our recent results indicate that PatS and other signals that regulate heterocyst pattern cannot interact, directly or indirectly, with a R223W mutant of HetR. We plan to use biochemical and genetic approaches to identify proteins that interact with the HetR protein, which will help reveal the mechanisms underlying its regulation of development. Our second goal is to determine how the nif genes are expressed. It is important to understand the mechanisms controlling nif genes since they represent the culmination of the differentiation process and the essence of heterocyst function. The Anabaena genome lacks the genes required for expression of nif genes present in other organisms such as rpoN (sigma 54

Summary of Disposable Debris Shields (DDS) Analysis for Development of Solid Debris Collection at NIF

International Nuclear Information System (INIS)

Shaughnessy, D.A.; Moody, K.J.; Grant, P.M.; Lewis, L.A.; Hutcheon, I.D.; Lindvall, R.; Gostic, J.M.

2011-01-01

Collection of solid debris from the National Ignition Facility (NIF) is being developed both as a diagnostic tool and as a means for measuring nuclear reaction cross sections relevant to the Stockpile Stewardship Program and nuclear astrophysics. The concept is straightforward; following a NIF shot, the debris that is produced as a result of the capsule and hohlraum explosion would be collected and subsequently extracted from the chamber. The number of nuclear activations that occurred in the capsule would then be measured through a combination of radiation detection and radiochemical processing followed by mass spectrometry. Development of the catcher is challenging due to the complex environment of the NIF target chamber. The collector surface is first exposed to a large photon flux, followed by the debris wind that is produced. The material used in the catcher must be mechanically strong in order to withstand the large amount of energy it is exposed to, as well as be chemically compatible with the form and composition of the debris. In addition, the location of the catcher is equally important. If it is positioned too close to the center of the target chamber, it will be significantly ablated, which could interfere with the ability of the debris to reach the surface and stick. If it is too far away, the fraction of the debris cloud collected will be too small to result in a statistically significant measurement. Material, geometric configuration, and location must all be tested in order to design the optimal debris collection system for NIF. One of the first ideas regarding solid debris collection at NIF was to use the disposable debris shields (DDS), which are fielded over the final optics assemblies (FOA) 7 m away from the center of the target chamber. The DDS are meant to be replaced after a certain number of shots, and if the shields could be subsequently analyzed after removal, it would serve as a mechanism for fielding a relatively large collection area

An assessment of the 3D geometric surrogacy of shock timing diagnostic techniques for tuning experiments on the NIF

Science.gov (United States)

Robey, H. F.; Munro, D. H.; Spears, B. K.; Marinak, M. M.; Jones, O. S.; Patel, M. V.; Haan, S. W.; Salmonson, J. D.; Landen, O. L.; Boehly, T. R.; Nikroo, A.

2008-05-01

Ignition capsule implosions planned for the National Ignition Facility (NIF) require a pulse shape with a carefully designed series of four steps, which launch a corresponding series of shocks through the ablator and DT ice shell. The relative timing of these shocks is critical for maintaining the DT fuel on a low adiabat. The current NIF specification requires that the timing of all four shocks be tuned to an accuracy of surrogacy of this modified geometry, 3D simulations using HYDRA [1] have been performed. The results from simulations of a quarter of the target geometry are presented. Comparisons of the hohlraum drive conditions and the resulting effect on the shock timing in the keyhole hohlraum are compared with the corresponding results for the standard ignition hohlraum.

13 CFR 305.5 - Project administration by District Organization.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Project administration by District Organization. 305.5 Section 305.5 Business Credit and Assistance ECONOMIC DEVELOPMENT ADMINISTRATION... business exists that could administer the Project in a more efficient or cost-effective manner than the...

The effect of laser pulse shape variations on the adiabat of NIF capsule implosions

Energy Technology Data Exchange (ETDEWEB)

Robey, H. F.; MacGowan, B. J.; Landen, O. L.; LaFortune, K. N.; Widmayer, C.; Celliers, P. M.; Moody, J. D.; Ross, J. S.; Ralph, J.; LePape, S.; Berzak Hopkins, L. F.; Spears, B. K.; Haan, S. W.; Clark, D.; Lindl, J. D.; Edwards, M. J. [LLNL, Livermore, California 94550 (United States)

2013-05-15

Indirectly driven capsule implosions on the National Ignition Facility (NIF) [Moses et al., Phys. Plasmas 16, 041006 (2009)] are being performed with the goal of compressing a layer of cryogenic deuterium-tritium (DT) fuel to a sufficiently high areal density (ρR) to sustain the self-propagating burn wave that is required for fusion power gain greater than unity. These implosions are driven with a temporally shaped laser pulse that is carefully tailored to keep the DT fuel on a low adiabat (ratio of fuel pressure to the Fermi degenerate pressure). In this report, the impact of variations in the laser pulse shape (both intentionally and unintentionally imposed) on the in-flight implosion adiabat is examined by comparing the measured shot-to-shot variations in ρR from a large ensemble of DT-layered ignition target implosions on NIF spanning a two-year period. A strong sensitivity to variations in the early-time, low-power foot of the laser pulse is observed. It is shown that very small deviations (∼0.1% of the total pulse energy) in the first 2 ns of the laser pulse can decrease the measured ρR by 50%.

General aspects of project organization

International Nuclear Information System (INIS)

Staebler, K.

1975-01-01

For power plant construction and errection the Federal Republic of Germany has a very well experienced industry with remarkable results in the past time. The 'bottle-neck' is not so much the design and manufacturing, it is also not in the area of sub-suppliers but it is more the personal for mounting and commissioning of the plant. In the report are only touched the various problems of project organization by the point of view of the utility. (orig./FW) [de

Project-Based Organizations and the New Spirit of Capitalism

DEFF Research Database (Denmark)

Jagd, Søren

Luc Boltanski and Eve Chiapello’s analysis of recent changes in capitalism – Le nouvel esprit du capitalisme - is an important contribution to the sociological analysis of capitalism. The aim of this paper is to present their main argument. It is argued that a particular important aspect...... of their analysis is the ideal-typical model of the new spirit of capitalism, the ideological underpinning of project-based organizations in ‘The Project World’. It is argued that the ideal-typical model of the Project World is the most elaborated description of the ‘cultural code’ of the emergent form...... of capitalist organizations. Last, it is discussed how we may obtain empirical evidence to test the hypothesis of a major change in capitalist organizational forms as well as in the recent spirit of capitalism....

Metagenomic of Actinomycetes Based on 16S rRNA and nifH Genes in Soil and Roots of Four Indonesian Rice Cultivars Using PCR-DGGE

Directory of Open Access Journals (Sweden)

Mahyarudin

2015-07-01

Full Text Available The research was conducted to study the metagenomic of actinomycetes based on 16S ribosomal RNA (rRNA and bacterial nifH genes in soil and roots of four rice cultivars. The denaturing gradient gel electrophoresis profile based on 16S rRNA gene showed that the diversity of actinomycetes in roots was higher than soil samples. The profile also showed that the diversity of actinomycetes was similar in four varieties of rice plant and three types of agroecosystem. The profile was partially sequenced and compared to GenBank database indicating their identity with closely related microbes. The blast results showed that 17 bands were closely related ranging from 93% to 100% of maximum identity with five genera of actinomycetes, which is Geodermatophilus, Actinokineospora, Actinoplanes, Streptomyces and Kocuria. Our study found that Streptomyces species in soil and roots of rice plants were more varied than other genera, with a dominance of Streptomyces alboniger and Streptomyces acidiscabies in almost all the samples. Bacterial community analyses based on nifH gene denaturing gradient gel electrophoresis showed that diversity of bacteria in soils which have nifH gene was higher than that in rice plant roots. The profile also showed that the diversity of those bacteria was similar in four varieties of rice plant and three types of agroecosystem. Five bands were closely related with nifH gene from uncultured bacterium clone J50, uncultured bacterium clone clod-38, and uncultured bacterium clone BG2.37 with maximum identity 99%, 98%, and 92%, respectively. The diversity analysis based on 16S rRNA gene differed from nifH gene and may not correlate with each other. The findings indicated the diversity of actinomycetes and several bacterial genomes analyzed here have an ability to fix nitrogen in soil and roots of rice plant.

Combining a thermal-imaging diagnostic with an existing imaging VISAR diagnostic at the National Ignition Facility (NIF)

International Nuclear Information System (INIS)

Robert M, Malone; John R, Celesteb; Peter M, Celliers; Brent C, Froggeta; Robert L, Guyton; Morris I, Kaufman; Tony L, Lee; Brian J, MacGowan; Edmund W, Ng; Imants P, Reinbachs; Ronald B, Robinson; Lynn G, Seppala; Tom W, Tunnell; Phillip W, Watts

2005-01-01

Optical diagnostics are currently being designed to analyze high-energy density physics experiments at the National Ignition Facility (NIF). Two independent line-imaging Velocity Interferometer System for Any Reflector (VISAR) interferometers have been fielded to measure shock velocities, breakout times, and emission of targets having sizes of 1-5 mm. An 8-inch-diameter, fused silica triplet lens collects light at f/3 inside the 30-foot-diameter NIF vacuum chamber. VISAR recordings use a 659.5-nm probe laser. By adding a specially coated beam splitter to the interferometer table, light at wavelengths from 540 to 645 nm is spilt into a thermal-imaging diagnostic. Because fused silica lenses are used in the first triplet relay, the intermediate image planes for different wavelengths separate by considerable distances. A corrector lens on the interferometer table reunites these separated wavelength planes to provide a good image. Thermal imaging collects light at f/5 from a 2-mm object placed at Target Chamber Center (TCC). Streak cameras perform VISAR and thermal-imaging recording. All optical lenses are on kinematic mounts so that pointing accuracy of the optical axis may be checked. Counter-propagating laser beams (orange and red) are used to align both diagnostics. The red alignment laser is selected to be at the 50 percent reflection point of the beam splitter. This alignment laser is introduced at the recording streak cameras for both diagnostics and passes through this special beam splitter on its way into the NIF vacuum chamber

APOM-project : an investigation of pharmacy organization and management

NARCIS (Netherlands)

Mobach, Mark P.; Werf, Jos J. van der; Tromp, T.F.J.

1998-01-01

Recently, in a joint cooperation of Stichting VNA, SAL Apotheken, the Faculty of Management and Organization, and the University Centre for Pharmacy, University of Groningen in the Netherlands, a Ph.D-study started regarding Apot(he)ek, Organization and Management (APOM). The APOM-project deals with

Multiple Perspectives on Organizing: projects between tyranny and perforation

DEFF Research Database (Denmark)

Koch, Christian; Bendixen, Mads

2005-01-01

engineering organizations are the need for multiple organizing principles and management to bridge contradictory and competing concerns for skill development, resource alignment and innovation efforts; and a strong need to focus on soft management practices such as mediation, brokering and coaching....... its projects and the spatial/community dimension. This enables an understanding of the multiple, often contrasting, organizing dynamics in the organization as well as diverse interests and groups found within this type of organization. It finds that it is not only the clients of the company who rule...

Recent results from the first polar direct drive plastic capsule implosions on NIF

Science.gov (United States)

Schmitt, Mark J.

2012-10-01

Polar direct drive (PDD) offers a simplified platform for conducting strongly driven implosions on NIF to investigate mix, hydro-burn and ignition-relevant physics. Its successful use necessitates a firm understanding and predictive capability of its implosion characteristics including hydro performance, symmetry and yield. To assess this capability, the first two PDD implosions of deuterium filled CH capsules were recently conducted at NIF. The P2 Legendre mode symmetry seen in these implosions agreed with pre-shot predictions even though the 700kJ drive energy produced intensities that far exceeded thresholds for both Raman and Brillouin stimulated scattering. These shots were also the first to employ image backlighting driven by two laser quads. Preliminary results indicate that the yield from the uncoated 2.25 mm diameter, 42 μm thick, CH shells was reduced by about a factor of two owing to as-shot laser drive asymmetries. Similarly, a small (sim50 μm) centroid offset between the upper and lower shell hemispheres seen in the first shot appears to be indicative of the laser quad energies. Overall, the implosion trajectories agreed with pre-shot predictions of bangtime. The second shot incorporated an 80 ?m wide,10 ?m deep depression encircling the equator of the capsule. This engineered feature was imposed to test our capability to predict the effect of high-mode features on yield and mix. A predicted yield reduction factor of 3 was not observed.[4pt] In collaboration with P. A. Bradley, J. A. Cobble, P. Hakel, S. C. Hsu, N. S. Krasheninnikova, G. A. Kyrala, G. R. Magelssen, T. J. Murphy, K. A. Obrey, R. C. Shah, I. L. Tregillis and F. J. Wysocki of Los Alamos National Laboratory; M. Marinak, R. Wallace, T. Parham, M. Cowan, S. Glenn, R. Benedetti and the NIF Operations Team of Lawrence Livermore National Laboratory; R. S. Craxton and P. W. McKenty of the Univ. Rochester; P. Fitzsimmons and A. Nikroo of General Atomics; H. Rinderknecht, M. Rosenberg, and M. G

Static and time-resolved 10-1000 keV x-ray imaging detector options for NIF

International Nuclear Information System (INIS)

Landen, O.L.; Bell, P.M.; McDonald, J.W.; Park, H.-S.; Weber, F.; Moody, J.D.; Lowry, M.E.; Stewart, R.E.

2004-01-01

High energy (>10 keV) x-ray self-emission imaging and radiography will be essential components of many NIF high energy density physics experiments. In preparation for such experiments, we have evaluated the pros and cons of various static [x-ray film, bare charge-coupled device (CCD), and scintillator + CCD] and time-resolved (streaked and gated) 10-1000 keV detectors

IT PROJECT PORTFOLIO MANAGEMENT: MODULARITY PROBLEMS IN A PUBLIC ORGANIZATION

DEFF Research Database (Denmark)

Hansen, Lars Kristian; Mengiste, Shegaw Anagaw

2012-01-01

As today’s public and private sector organizations heavily rely on Information Technology (IT) to provide faster cycle times and better services, IT Project Portfolio Management (IT PPM) has become a high priority issue. This research adopts engaged scholarship to investigate IT PPM practices...... within a large local government. The investigation applies Modularity theory to analyze rich data from the local government covering several units with quite diverse functions to address the following two questions (1) which modularity problems does a public organization have in its IT PPM practices...... suggest a model addressing the identified problems by organizing IT PPM in three modules connected by three gateways. These results may be used to inform further research into IT PPM and to help managers improve IT PPM practices in public organizations. Keywords: IT Project Portfolio Management (IT PPM...

High-resolution 3D simulations of NIF ignition targets performed on Sequoia with HYDRA

Science.gov (United States)

Marinak, M. M.; Clark, D. S.; Jones, O. S.; Kerbel, G. D.; Sepke, S.; Patel, M. V.; Koning, J. M.; Schroeder, C. R.

2015-11-01

Developments in the multiphysics ICF code HYDRA enable it to perform large-scale simulations on the Sequoia machine at LLNL. With an aggregate computing power of 20 Petaflops, Sequoia offers an unprecedented capability to resolve the physical processes in NIF ignition targets for a more complete, consistent treatment of the sources of asymmetry. We describe modifications to HYDRA that enable it to scale to over one million processes on Sequoia. These include new options for replicating parts of the mesh over a subset of the processes, to avoid strong scaling limits. We consider results from a 3D full ignition capsule-only simulation performed using over one billion zones run on 262,000 processors which resolves surface perturbations through modes l = 200. We also report progress towards a high-resolution 3D integrated hohlraum simulation performed using 262,000 processors which resolves surface perturbations on the ignition capsule through modes l = 70. These aim for the most complete calculations yet of the interactions and overall impact of the various sources of asymmetry for NIF ignition targets. This work was performed under the auspices of the Lawrence Livermore National Security, LLC, (LLNS) under Contract No. DE-AC52-07NA27344.

Comparisons of NIF convergent ablation simulations with radiograph data.

Science.gov (United States)

Olson, R E; Hicks, D G; Meezan, N B; Koch, J A; Landen, O L

2012-10-01

A technique for comparing simulation results directly with radiograph data from backlit capsule implosion experiments will be discussed. Forward Abel transforms are applied to the kappa*rho profiles of the simulation. These provide the transmission ratio (optical depth) profiles of the simulation. Gaussian and top hat blurs are applied to the simulated transmission ratio profiles in order to account for the motion blurring and imaging slit resolution of the experimental measurement. Comparisons between the simulated transmission ratios and the radiograph data lineouts are iterated until a reasonable backlighter profile is obtained. This backlighter profile is combined with the blurred, simulated transmission ratios to obtain simulated intensity profiles that can be directly compared with the radiograph data. Examples will be shown from recent convergent ablation (backlit implosion) experiments at the NIF.

NIF target fill, transport and insertion cryostat

International Nuclear Information System (INIS)

Warren, R.

1994-01-01

A cryostat to support the fielding of a cryogenic target within the NIF is described. The present design is predicated upon fuel layer symmetry being achieved with the β layering process and modifications needed for other fuel symmetrization processes are discussed. These include the vertically differentially heated capsule with a uniform liquid layer stabilized by a surface tension gradient, foam supported liquid layers and solid D 2 or HD layers symmetrized by bulk irradiation from a laser source. The cold sinks to be incorporated in these techniques could, in principal, be cooled with the high pressure helium envisioned for the heat sink rings of the present design. Supplementary laser access would be provided for differential heating of the capsule for surface tension gradient stabilization of a liquid layer or bulk heating of a solid layer. The cryostat in each of these cases would look substantially the same as in the present case with the only significant differences being in the details of the design in the immediate vicinity of the target

Beam control and diagnostic functions in the NIF transport spatial filter

International Nuclear Information System (INIS)

Holdener, F.R.; Ables, E.; Bliss, E.S.

1996-10-01

Beam control and diagnostic systems are required to align the National Ignition Facility (NIF) laser prior to a shot as well as to provide diagnostics on 192 beam lines at shot time. A design that allows each beam's large spatial filter lenses to also serve as objective lenses for beam control and diagnostic sensor packages helps to accomplish the task at a reasonable cost. However, this approach also causes a high concentration of small optics near the pinhole plane of the transport spatial filter (TSF) at the output of each beam. This paper describes the optomechanical design in and near the central vacuum vessel of the TSF

Building Bridges between Science Courses Using Honors Organic Chemistry Projects

Science.gov (United States)

Hickey, Timothy; Pontrello, Jason

2016-01-01

Introductory undergraduate science courses are traditionally offered as distinct units without formalized student interaction between classes. To bridge science courses, the authors used three Honors Organic Chemistry projects paired with other science courses. The honors students delivered presentations to mainstream organic course students and…

Handbook of selected organ doses for projections common in pediatric radiology

International Nuclear Information System (INIS)

Rosenstein, M.; Beck, T.J.; Warner, G.G.

1979-05-01

This handbook contains data from which absorbed dose (mrad) to selected organs can be estimated for common projections in pediatric radiology. The organ doses are for three reference patients: a newborn (0 to 6 months), a 1-year old child, and a 5-year old child. One intent of the handbook is to permit the user to evaluate the effect on organ dose to these reference pediatric patients as a function of certain changes in technical parameters used in or among facilities. A second intent is to permit a comparison to be made of organ doses as a function of age. This comparison can be extended to a reference adult by referring to the previous Handbook of Selected Organ Doses fo Projections Common in Diagnostic Radiology, FDA 76-8031. Assignment of organ doses to individual pediatric patients using the Handbook data is not recommended unless the physical characteristics of the patient closely correlate with one of the three reference pediatric patients given in Appendix A

 Project Management as a Global Trend for Organization Work

DEFF Research Database (Denmark)

Kampf, Constance

in multination and global companies, understanding the power of visual rhetoric, genre and writing processes in the context of project management documentation can be an advantage for technical communicators.  In addition, project management tools and online documentation spaces are objects which cross...... Project Management as a Global Trend for Organization Work: Implications for Technical Communication Project Management tools and processes offer a visual approach to producing knowledge about a project in order to complete it.  As project management practices are used with increasing frequency......-cultural teams use to function.  This presentation will explore the potential of Project Management to be tightly integrated in Technical Communication curricula through a communications approach to project management.  Questions for discussion include: How tightly is project management integrated into different...

SemanticOrganizer: A Customizable Semantic Repository for Distributed NASA Project Teams

Science.gov (United States)

Keller, Richard M.; Berrios, Daniel C.; Carvalho, Robert E.; Hall, David R.; Rich, Stephen J.; Sturken, Ian B.; Swanson, Keith J.; Wolfe, Shawn R.

2004-01-01

SemanticOrganizer is a collaborative knowledge management system designed to support distributed NASA projects, including diverse teams of scientists, engineers, and accident investigators. The system provides a customizable, semantically structured information repository that stores work products relevant to multiple projects of differing types. SemanticOrganizer is one of the earliest and largest semantic web applications deployed at NASA to date, and has been used in diverse contexts ranging from the investigation of Space Shuttle Columbia's accident to the search for life on other planets. Although the underlying repository employs a single unified ontology, access control and ontology customization mechanisms make the repository contents appear different for each project team. This paper describes SemanticOrganizer, its customization facilities, and a sampling of its applications. The paper also summarizes some key lessons learned from building and fielding a successful semantic web application across a wide-ranging set of domains with diverse users.

Integration and the hold-up problem in the design organization for engineering projects

NARCIS (Netherlands)

Zerjav, Vedran; Hartmann, Timo; Javernick-Will, A.; Chinowsky, P.

2012-01-01

The paper presents a perspective of the design organization in engineering projects based on the economic concept of the hold-up problem. By integrating the economic theories on the boundaries of organizations into the existing knowledge on design in engineering projects, the paper hypothesizes a

Imaging of high-energy x-ray emission from cryogenic thermonuclear fuel implosions on the NIF.

Science.gov (United States)

Ma, T; Izumi, N; Tommasini, R; Bradley, D K; Bell, P; Cerjan, C J; Dixit, S; Döppner, T; Jones, O; Kline, J L; Kyrala, G; Landen, O L; LePape, S; Mackinnon, A J; Park, H-S; Patel, P K; Prasad, R R; Ralph, J; Regan, S P; Smalyuk, V A; Springer, P T; Suter, L; Town, R P J; Weber, S V; Glenzer, S H

2012-10-01

Accurately assessing and optimizing the implosion performance of inertial confinement fusion capsules is a crucial step to achieving ignition on the NIF. We have applied differential filtering (matched Ross filter pairs) to provide broadband time-integrated absolute x-ray self-emission images of the imploded core of cryogenic layered implosions. This diagnostic measures the temperature- and density-sensitive bremsstrahlung emission and provides estimates of hot spot mass, mix mass, and pressure.

Imaging of high-energy x-ray emission from cryogenic thermonuclear fuel implosions on the NIF

Energy Technology Data Exchange (ETDEWEB)

Ma, T.; Izumi, N.; Tommasini, R.; Bradley, D. K.; Bell, P.; Cerjan, C. J.; Dixit, S.; Doeppner, T.; Jones, O.; Landen, O. L.; LePape, S.; Mackinnon, A. J.; Park, H.-S.; Patel, P. K.; Prasad, R. R.; Ralph, J.; Smalyuk, V. A.; Springer, P. T.; Suter, L.; Town, R. P. J. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); and others

2012-10-15

Accurately assessing and optimizing the implosion performance of inertial confinement fusion capsules is a crucial step to achieving ignition on the NIF. We have applied differential filtering (matched Ross filter pairs) to provide broadband time-integrated absolute x-ray self-emission images of the imploded core of cryogenic layered implosions. This diagnostic measures the temperature- and density-sensitive bremsstrahlung emission and provides estimates of hot spot mass, mix mass, and pressure.

Design calculations for a xenon plasma x-ray shield to protect the NIF optical Thomson scattering diagnostic.

Science.gov (United States)

Swadling, G F; Ross, J S; Datte, P; Moody, J; Divol, L; Jones, O; Landen, O

2016-11-01

An Optical Thomson Scattering (OTS) diagnostic is currently being developed for the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory. This diagnostic is designed to make measurements of the hohlraum plasma parameters, such as the electron temperature and the density, during inertial confinement fusion (ICF) experiments. NIF ICF experiments present a very challenging environment for optical measurements; by their very nature, hohlraums produce intense soft x-ray emission, which can cause "blanking" (radiation induced opacity) of the radiation facing optical components. The soft x-ray fluence at the surface of the OTS blast shield, 60 cm from the hohlraum, is estimated to be ∼8 J cm -2 . This is significantly above the expected threshold for the onset of "blanking" effects. A novel xenon plasma x-ray shield is proposed to protect the blast shield from x-rays and mitigate "blanking." Estimates suggest that an areal density of 10 19 cm -2 Xe atoms will be sufficient to absorb 99.5% of the soft x-ray flux. Two potential designs for this shield are presented.

Collecting lessons learned : How project-based organizations in the oil and gas industry learn from their projects

NARCIS (Netherlands)

Buttler, T.

2016-01-01

Project-based organizations collect lessons learned in order to improve the performance of projects. They aim to repeat successes by using positive lessons learned, and to avoid repeating negative experiences by using negative lessons learned. Cooke-Davies (2002) claimed that the ability to learn

Comparison of plastic, high density carbon, and beryllium as indirect drive NIF ablators

Science.gov (United States)

Kritcher, A. L.; Clark, D.; Haan, S.; Yi, S. A.; Zylstra, A. B.; Callahan, D. A.; Hinkel, D. E.; Berzak Hopkins, L. F.; Hurricane, O. A.; Landen, O. L.; MacLaren, S. A.; Meezan, N. B.; Patel, P. K.; Ralph, J.; Thomas, C. A.; Town, R.; Edwards, M. J.

2018-05-01

Detailed radiation hydrodynamic simulations calibrated to experimental data have been used to compare the relative strengths and weaknesses of three candidate indirect drive ablator materials now tested at the NIF: plastic, high density carbon or diamond, and beryllium. We apply a common simulation methodology to several currently fielded ablator platforms to benchmark the model and extrapolate designs to the full NIF envelope to compare on a more equal footing. This paper focuses on modeling of the hohlraum energetics which accurately reproduced measured changes in symmetry when changes to the hohlraum environment were made within a given platform. Calculations suggest that all three ablator materials can achieve a symmetric implosion at a capsule outer radius of ˜1100 μm, a laser energy of 1.8 MJ, and a DT ice mass of 185 μg. However, there is more uncertainty in the symmetry predictions for the plastic and beryllium designs. Scaled diamond designs had the most calculated margin for achieving symmetry and the highest fuel absorbed energy at the same scale compared to plastic or beryllium. A comparison of the relative hydrodynamic stability was made using ultra-high resolution capsule simulations and the two dimensional radiation fluxes described in this work [Clark et al., Phys. Plasmas 25, 032703 (2018)]. These simulations, which include low and high mode perturbations, suggest that diamond is currently the most promising for achieving higher yields in the near future followed by plastic, and more data are required to understand beryllium.

Impact of a quality improvement project on deceased organ donor management

Science.gov (United States)

Olmos, Andrea; Feiner, John; Hirose, Ryutaro; Swain, Sharon; Blasi, Annabel; Roberts, John P.; Niemann, Claus U.

2017-01-01

Context Donors showed poor glucose control in the period between declaration of brain death and organ recovery. The level of hyperglycemia in the donors was associated with a decline in terminal renal function. Objective To determine whether implementation of a quality improvement project improved glucose control and preserved renal function in deceased organ donors. Methods Data collected retrospectively included demographics, medical history, mechanism of death, laboratory values, and data from the United Network for Organ Sharing. Results After implementation of the quality improvement project, deceased donors had significantly lower mean glucose concentrations (mean [SD], 162 [44] vs 212 [42] mg/dL; P donor cohorts from before and after the quality improvement project were analyzed together, mean glucose concentration remained a significant predictor of terminal creatinine level (P donors indicated that higher terminal creatinine level was associated with delayed graft function in recipients (P donor glucose homeostasis, and the data confirm that poor glucose homeostasis is associated with worsening terminal renal function. PMID:26645930

Calibration of a High Resolution X-ray Spectrometer for High-Energy-Density Plasmas on NIF

Science.gov (United States)

Kraus, B.; Gao, L.; Hill, K. W.; Bitter, M.; Efthimion, P.; Schneider, M. B.; Chen, H.; Ayers, J.; Beiersdorfer, P.; Liedahl, D.; Macphee, A. G.; Thorn, D. B.; Bettencourt, R.; Kauffman, R.; Le, H.; Nelson, D.

2017-10-01

A high-resolution, DIM-based (Diagnostic Instrument Manipulator) x-ray crystal spectrometer has been calibrated for and deployed at the National Ignition Facility (NIF) to diagnose plasma conditions and mix in ignition capsules near stagnation times. Two conical crystals in the Hall geometry focus rays from the Kr He- α, Ly- α, and He- β complexes onto a streak camera for time-resolved spectra, in order to measure electron density and temperature by observing Stark broadening and relative intensities of dielectronic satellites. Signals from these two crystals are correlated with a third crystal that time-integrates the intervening energy range. The spectrometer has been absolutely calibrated using a microfocus x-ray source, an array of CCD and single-photon-counting detectors, and K- and L-absorption edge filters. Measurements of the integrated reflectivity, energy range, and energy resolution for each crystal will be presented. The implications of the calibration on signal levels from NIF implosions and x-ray filter choices will be discussed. This work was performed under the auspices of the U.S. DoE by Princeton Plasma Physics Laboratory under contract DE-AC02-09CH11466 and by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344.

Selecting Large Portfolios of Social Projects in Public Organizations

Directory of Open Access Journals (Sweden)

Igor Litvinchev

2014-01-01

Full Text Available We address the portfolio selection of social projects in public organizations considering interdependencies (synergies affecting project funds requirements and tasks. A mixed integer linear programming model is proposed incorporating the most relevant aspects of the problem found in the literature. The model supports both complete (all or nothing and partial (a certain amount from a given interval of funding resource allocation policies. Numerical results for large-scale problem instances are presented.

A Conceptualization of Knowledge Governance in Project-based Organizations

DEFF Research Database (Denmark)

Pemsel, Sofia; Wiewiorac, Anna; Müller, Ralf

2014-01-01

This paper conceptualizes and defines knowledge governance (KG) in project-based organizations (PBOs). Two key contributions towards a multi-faceted view of KG and an understanding of KG in PBOs are advanced, as distinguished from knowledge management and organizational learning concepts. The con......This paper conceptualizes and defines knowledge governance (KG) in project-based organizations (PBOs). Two key contributions towards a multi-faceted view of KG and an understanding of KG in PBOs are advanced, as distinguished from knowledge management and organizational learning concepts....... The conceptual framework addresses macro- and micro-level elements of KG and their interaction. Our definition of KG in PBOs highlights the contingent nature of KG processes in relation to their organizational context. These contributions provide a novel platform for understanding KG in PBOs....

Spatial conditions - the 3. supervisor in project organized teaching?

DEFF Research Database (Denmark)

Bering Keiding, Tina

through reflection of its own border: is this new issue inside or outside the project. Individual rooms make it easier for the project to stabilize around its eigenvalues, but also enhance the risk of cognitive jog-trot. The challenge in this case becomes to seek out disturbances which can initiate new......The contribution presents a pilot study on project work situated in different spatial contexts. The main question for the study is if and how spatial conditions influence the interaction within and between projects. Possible answers to the question are sought through observations, informal...... interviews and questionnaires. The empirical findings are reflected in a Luhmann-based re-description of project organized group based teaching (Keiding 2008). Preliminary conclusions indicate that one big difference between open-space studio and individual group rooms is related to the immediate public...

The project organization as a policy tool in implementing welfare reforms in the public sector.

Science.gov (United States)

Jensen, Christian; Johansson, Staffan; Löfström, Mikael

2013-01-01

Organizational design is considered in policy literature as a forceful policy tool to put policy to action. However, previous research has not analyzed the project organization as a specific form of organizational design and, hence, has not given much attention to such organizations as a strategic choice when selecting policy tools. The purpose of the article is to investigate the project as a policy tool; how do such temporary organizations function as a specific form of organization when public policy is implemented? The article is based on a framework of policy implementation and is illustrated with two welfare reforms in the Swedish public sector, which were organized and implemented as project organizations. The case studies and the analysis show that it is crucial that a project organization fits into the overall governance structure when used as a policy tool. If not, the project will remain encapsulated and will not have sufficient impact on the permanent organizational structure. The concept of encapsulation indicates a need to protect the project from a potential hostile environment. The implication of this is that organizational design as a policy tool is a matter that deserves more attention in the strategic discussion on implementing public policies and on the suitability of using certain policy tools. Copyright © 2012 John Wiley & Sons, Ltd.

Online tutoring procedure for research project supervision: management, organization and key elements

Directory of Open Access Journals (Sweden)

Antònia Darder Mesquida

2015-07-01

Full Text Available Research project tutoring appears as a crucial element for teaching; it is a planned action based on the relationship between a tutor and a student. This paper presents the findings of a design and development research which has as its main aim to create an organization system for the tutoring of online research projects. That system seeks to facilitate the tutoring and supervision task with trainee researchers, providing guidance for its management and instruments for its implementation. The main conclusions arising from this research derive from considering the need to offer a solution to the problem of distance research project supervision and has materialized in organization and sequencing through a model about the variables that influence the research project tutoring problem.

Conceptual design of the National Ignition Facility

International Nuclear Information System (INIS)

Paisner, J.A.; Kumpan, S.A.; Lowdermilk, W.H.; Boyes, J.D.; Sorem, M.

1995-01-01

DOE commissioned a Conceptual Design Report (CDR) for the National Ignition Facility (NIF) in January 1993 as part of a Key Decision Zero (KDO), justification of Mission Need. Motivated by the progress to date by the Inertial Confinement Fusion (ICF) program in meeting the Nova Technical Contract goals established by the National Academy of Sciences in 1989, the Secretary requested a design using a solid-state laser driver operating at the third harmonic (0.35 μm) of neodymium (Nd) glass. The participating ICF laboratories signed a Memorandum of Agreement in August 1993, and established a Project organization, including a technical team from the Lawrence Livermore National Laboratory (LLNL), Los Alamos National Laboratory (LANL), Sandia National Laboratories (SNL), and the Laboratory for Laser Energetics at the University of Rochester. Since then, we completed the NIF conceptual design, based on standard construction at a generic DOE Defense Program's site, and issued a 7,000-page, 27-volume CDR in May 1994.2 Over the course of the conceptual design study, several other key documents were generated, including a Facilities Requirements Document, a Conceptual Design Scope and Plan, a Target Physics Design Document, a Laser Design Cost Basis Document, a Functional Requirements Document, an Experimental Plan for Indirect Drive Ignition, and a Preliminary Hazards Analysis (PHA) Document. DOE used the PHA to categorize the NIF as a low-hazard, non-nuclear facility. On October 21, 1994 the Secretary of Energy issued a Key Decision One (KD1) for the NIF, which approved the Project and authorized DOE to request Office of Management and Budget-approval for congressional line-item FY 1996 NIF funding for preliminary engineering design and for National Environmental Policy Act activities. In addition, the Secretary declared Livermore as the preferred site for constructing the NIF. The Project will cost approximately $1.1 billion and will be completed at the end of FY 2002

Information System Development: Can Traditional Project Management Tools Be Successful in Post-Industrial Organizations?

DEFF Research Database (Denmark)

Carugati, Andrea

2002-01-01

Literature on project management of information systems development suggests practices based on success stories in industrial organizations. Recent trends towards post-industrial organizational forms and loosely coupled networked organizations raise questions regarding the applicability of these ...... of information systems development projects in post-industrial organizations. The guidelines are both the result of post-rationalization of the team members and of the case analysis....

Implementing a Student-Designed Green Chemistry Laboratory Project in Organic Chemistry

Science.gov (United States)

Graham, Kate J.; Jones, T. Nicholas; Schaller, Chris P.; McIntee, Edward J.

2014-01-01

A multiweek organic chemistry laboratory project is described that emphasizes sustainable practices in experimental design. An emphasis on student-driven development of the project is meant to mirror the independent nature of research. Students propose environmentally friendly modifications of several reactions. With instructor feedback, students…

Upgrade and benchmarking of the NIFS physics-engineering-cost code

International Nuclear Information System (INIS)

Dolan, T.J.; Yamazaki, K.

2004-07-01

The NIFS Physics-Engineering-Cost (PEC) code for helical and tokamak fusion reactors is upgraded by adding data from three blanket-shield designs, a new cost section based on the ARIES cost schedule, more recent unit costs, and improved algorithms for various computations. The PEC code is also benchmarked by modeling the ARIES-AT (advanced technology) tokamak and the ARIES-SPPS (stellarator power plant system). The PEC code succeeds in predicting many of the pertinent plasma parameters and reactor component masses within about 10%. There are cost differences greater than 10% for some fusion power core components, which may be attributed to differences of unit costs used by the codes. The COEs estimated by the PEC code differ from the COEs of the ARIES-AT and ARIES-SPPS studies by 5%. (author)

Acute Radiation Risk and BRYNTRN Organ Dose Projection Graphical User Interface

Science.gov (United States)

Cucinotta, Francis A.; Hu, Shaowen; Nounu, Hateni N.; Kim, Myung-Hee

2011-01-01

The integration of human space applications risk projection models of organ dose and acute radiation risk has been a key problem. NASA has developed an organ dose projection model using the BRYNTRN with SUM DOSE computer codes, and a probabilistic model of Acute Radiation Risk (ARR). The codes BRYNTRN and SUM DOSE are a Baryon transport code and an output data processing code, respectively. The risk projection models of organ doses and ARR take the output from BRYNTRN as an input to their calculations. With a graphical user interface (GUI) to handle input and output for BRYNTRN, the response models can be connected easily and correctly to BRYNTRN. A GUI for the ARR and BRYNTRN Organ Dose (ARRBOD) projection code provides seamless integration of input and output manipulations, which are required for operations of the ARRBOD modules. The ARRBOD GUI is intended for mission planners, radiation shield designers, space operations in the mission operations directorate (MOD), and space biophysics researchers. BRYNTRN code operation requires extensive input preparation. Only a graphical user interface (GUI) can handle input and output for BRYNTRN to the response models easily and correctly. The purpose of the GUI development for ARRBOD is to provide seamless integration of input and output manipulations for the operations of projection modules (BRYNTRN, SLMDOSE, and the ARR probabilistic response model) in assessing the acute risk and the organ doses of significant Solar Particle Events (SPEs). The assessment of astronauts radiation risk from SPE is in support of mission design and operational planning to manage radiation risks in future space missions. The ARRBOD GUI can identify the proper shielding solutions using the gender-specific organ dose assessments in order to avoid ARR symptoms, and to stay within the current NASA short-term dose limits. The quantified evaluation of ARR severities based on any given shielding configuration and a specified EVA or other mission

Human resource management in the project-oriented organization: Employee well-being and ethical treatment

NARCIS (Netherlands)

Turner, R.; Huemann, M.; Keegan, A.

2008-01-01

As part of a wider study into human resource management (HRM) practices in project-oriented organizations, we investigated the issue of employee well-being. Project-oriented organizations adopt temporary work processes to deliver products and services to clients. This creates a dynamic work

The Development of Digital Collections and Resources Organization Related Projects in Taiwan

Directory of Open Access Journals (Sweden)

Hsueh-Hua Chen

2001-12-01

Full Text Available With the development of Internet, digital libraries/museums have received worldwide attention and many developed countries are doing extensive researches on digital libraries/museums. In Taiwan, many institutions have digitized their rare collections. This paper introduces the recent development of digital projects in Taiwan, including: Digital Museum Project, National Digital Collection Project and National Culture Database Project, and also especially introduces some resources organization related projects. [Article content in Chinese

Preliminary report: NIF laser bundle review

International Nuclear Information System (INIS)

Tietbohl, G.L.; Larson, D.W.; Erlandson, A.C.

1995-01-01

As requested in the guidance memo 1 , this committe determined whether there are compelling reasons to recommend a change from the NIF CDR baseline laser. The baseline bundle design based on a tradeoff between cost and technical risk, which is replicated four times to create the required 192 beams. The baseline amplifier design uses bottom loading 1x4 slab and flashlamp cassettes for amplifier maintenance and large vacuum enclosures (2.5m high x 7m wide in cross-section for each of the two spatial filters in each of the four bundles. The laser beams are arranged in two laser bays configured in a u-shape around the target area. The entire bundle review effort was performed in a very short time (six weeks) and with limited resources (15 personnel part-time). This should be compared to the effort that produced the CDR design (12 months, 50 to 100 personnel). This committee considered three alternate bundle configurations (2x2, 4x2, and 4x4 bundles), and evaluated each bundle against the baseline design using the seven requested issues in the guidance memo: Cost; schedule; performance risk; maintainability/operability; hardware failure cost exposure; activation; and design flexibility. The issues were reviewed to identify differences between each alternate bundle configuration and the baseline

Design calculations for a xenon plasma x-ray shield to protect the NIF optical Thomson scattering diagnostic

Energy Technology Data Exchange (ETDEWEB)

Swadling, G. F.; Ross, J. S.; Datte, P.; Moody, J.; Divol, L.; Jones, O.; Landen, O. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States)

2016-11-15

An Optical Thomson Scattering (OTS) diagnostic is currently being developed for the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory. This diagnostic is designed to make measurements of the hohlraum plasma parameters, such as the electron temperature and the density, during inertial confinement fusion (ICF) experiments. NIF ICF experiments present a very challenging environment for optical measurements; by their very nature, hohlraums produce intense soft x-ray emission, which can cause “blanking” (radiation induced opacity) of the radiation facing optical components. The soft x-ray fluence at the surface of the OTS blast shield, 60 cm from the hohlraum, is estimated to be ∼8 J cm{sup −2}. This is significantly above the expected threshold for the onset of “blanking” effects. A novel xenon plasma x-ray shield is proposed to protect the blast shield from x-rays and mitigate “blanking.” Estimates suggest that an areal density of 10{sup 19} cm{sup −2} Xe atoms will be sufficient to absorb 99.5% of the soft x-ray flux. Two potential designs for this shield are presented.

Deuterium-tritium neutron yield measurements with the 4.5 m neutron-time-of-flight detectors at NIF.

Science.gov (United States)

Moran, M J; Bond, E J; Clancy, T J; Eckart, M J; Khater, H Y; Glebov, V Yu

2012-10-01

The first several campaigns of laser fusion experiments at the National Ignition Facility (NIF) included a family of high-sensitivity scintillator∕photodetector neutron-time-of-flight (nTOF) detectors for measuring deuterium-deuterium (DD) and DT neutron yields. The detectors provided consistent neutron yield (Y(n)) measurements from below 10(9) (DD) to nearly 10(15) (DT). The detectors initially demonstrated detector-to-detector Y(n) precisions better than 5%, but lacked in situ absolute calibrations. Recent experiments at NIF now have provided in situ DT yield calibration data that establish the absolute sensitivity of the 4.5 m differential tissue harmonic imaging (DTHI) detector with an accuracy of ± 10% and precision of ± 1%. The 4.5 m nTOF calibration measurements also have helped to establish improved detector impulse response functions and data analysis methods, which have contributed to improving the accuracy of the Y(n) measurements. These advances have also helped to extend the usefulness of nTOF measurements of ion temperature and downscattered neutron ratio (neutron yield 10-12 MeV divided by yield 13-15 MeV) with other nTOF detectors.

Ultrasonic Testing of NIF Amplifier FAU Top Plates

International Nuclear Information System (INIS)

Chinn, D.J.; Huber, R.D.; Haskins, J.J.; Rodriguez, J.A.; Souza, P.R.; Le, T.V.

2002-01-01

A key component in the National Ignition Facility (NIF) laser optic system is the amplifier frame assembly unit (FAU). The cast aluminum top plate that supports the FAU is required to withstand loads that would occur during an earthquake with a recurrence period of 1000 years. The stringent seismic requirements placed on the FAU top plate induced a study of the cast aluminum material used in the top plate. Ultrasonic testing was used to aid in characterizing the aluminum material used in the plates. This report documents the work performed using contact ultrasonic testing to characterize the FAU top plate material. The ultrasonic work reported here had 3 objectives: (1) inspect the plate material before cyclic testing conducted at the Pacific Earthquake Engineering Research Center (PEER); (2) determine the overall quality of individual plates; and (3) detect large defects in critical areas of individual plates. Section III, ''Pre-cyclic test inspection'', describes work performed in support of Objective 1. Section IV, ''Ultrasonic field measurements'', describes work performed in support of Objectives 2 and 3

Ultra High Mode Mix in NIF NIC Implosions

Science.gov (United States)

Scott, Robbie; Garbett, Warren

2017-10-01

This work re-examines a sub-set of the low adiabat implosions from the National Ignition Campaign in an effort to better understand potential phenomenological sources of `excess' mix observed experimentally. An extensive effort has been made to match both shock-timing and backlit radiography (Con-A) implosion data in an effort to reproduce the experimental conditions as accurately as possible. Notably a 30% reduction in ablation pressure at peak drive is required to match the experimental data. The reduced ablation pressure required to match the experimental data allows the ablator to decompress, in turn causing the DT ice-ablator interface to go Rayleigh-Taylor unstable early in the implosion acceleration phase. Post-processing the runs with various mix models indicates high-mode mix from the DT ice-ablator interface may penetrate deep into the hotspot. This work offers a potential explanation of why these low-adiabat implosions exhibited significantly higher levels of mix than expected from high-fidelity multi-dimensional simulations. Through this new understanding, a possible route forward for low-adiabat implosions on NIF is suggested.

Problems and Concerns Regarding Access Control System Construction in Radiation Facilities Based on the NIFS Experience

International Nuclear Information System (INIS)

Kawano, T.; Inoue, N.; Sakuma, Y.; Motojima, O.

2001-01-01

Full text: In 1998, access control system for the large helical device (LHD) experimental hall was constructed and put into operation at the National Institute for Fusion Science (NIFS) in Toki, Japan. Since then, the system has been continuously improved. It now controls access into the LHD controlled area through four entrances. The system has five turnstile gates and enables control of access at the four entrances. The system is always checking whether the shielding doors are open or closed at eight positions. The details pertaining to the construction of the system were reported at IRPA-10 held in Hiroshima, Japan, in 2000. Based on our construction experience of the NIFS access control system, we will discuss problems related to software and operational design of the system. We will also discuss some concerns regarding the use of the system in radiation facilities. The problems we will present concern, among other thing, individual registration, time control, turnstile control, interlock signal control, data aggregation and transactions, automatic and manual control, and emergency procedures. For example, in relation to the time control and turnstile control functions, we will discuss the gate-opening time interval for an access event, the timing of access data recording, date changing, turn bar control, double access, and access error handling. (author)

Prediction of scaling physics laws for proton acceleration with extended parameter space of the NIF ARC

Science.gov (United States)

Bhutwala, Krish; Beg, Farhat; Mariscal, Derek; Wilks, Scott; Ma, Tammy

2017-10-01

The Advanced Radiographic Capability (ARC) laser at the National Ignition Facility (NIF) at Lawrence Livermore National Laboratory is the world's most energetic short-pulse laser. It comprises four beamlets, each of substantial energy ( 1.5 kJ), extended short-pulse duration (10-30 ps), and large focal spot (>=50% of energy in 150 µm spot). This allows ARC to achieve proton and light ion acceleration via the Target Normal Sheath Acceleration (TNSA) mechanism, but it is yet unknown how proton beam characteristics scale with ARC-regime laser parameters. As theory has also not yet been validated for laser-generated protons at ARC-regime laser parameters, we attempt to formulate the scaling physics of proton beam characteristics as a function of laser energy, intensity, focal spot size, pulse length, target geometry, etc. through a review of relevant proton acceleration experiments from laser facilities across the world. These predicted scaling laws should then guide target design and future diagnostics for desired proton beam experiments on the NIF ARC. This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344 and funded by the LLNL LDRD program under tracking code 17-ERD-039.

Imaging of High-Energy X-Ray Emission from Cryogenic Thermonuclear Fuel Implosions on the NIF

International Nuclear Information System (INIS)

Ma, T.

2012-01-01

Accurately assessing and optimizing the implosion performance of inertial confinement fusion capsules is a crucial step to achieving ignition on the NIF. We have applied differential filtering (matched Ross filter pairs) to provide spectrally resolved time-integrated absolute x-ray self-emission images of the imploded core of cryogenic layered targets. Using bremsstrahlung assumptions, the measured absolute x-ray brightness allows for the inference of electron temperature, electron density, hot spot mass, mix mass, and pressure. Current inertial confinement fusion (ICF) experiments conducted on the National Ignition Facility (NIF) seek to indirectly drive a spherical implosion, compressing and igniting a deuterium-tritium fuel. This DT fuel capsule is cryogenically prepared as a solid ice layer surrounded by a low-Z ablator material. Ignition will occur when the hot spot approaches sufficient temperature (∼3-4 keV) and ρR (∼0.3 g/cm 2 ) such that alpha deposition can further heat the hot spot and generate a self-sustaining burn wave. During the implosion, the fuel mass becomes hot enough to emit large amounts of x-ray radiation, the spectra and spatial variation of which contains key information that can be used to evaluate the implosion performance. The Ross filter diagnostic employs differential filtering to provide spectrally resolved, time-integrated, absolute x-ray self-emission images of the imploded core of cryogenic layered targets.

3D Simulations of NIF Wetted Foam Experiments to Understand the Transition from 2D to 3D Implosion Behavior

Science.gov (United States)

Haines, Brian; Olson, Richard; Yi, Austin; Zylstra, Alex; Peterson, Robert; Bradley, Paul; Shah, Rahul; Wilson, Doug; Kline, John; Leeper, Ramon; Batha, Steve

2017-10-01

The high convergence ratio (CR) of layered Inertial Confinement Fusion capsule implosions contribute to high performance in 1D simulations yet make them more susceptible to hydrodynamic instabilities, contributing to the development of 3D flows. The wetted foam platform is an approach to hot spot ignition to achieve low-to-moderate convergence ratios in layered implosions on the NIF unobtainable using an ice layer. Detailed high-resolution modeling of these experiments in 2D and 3D, including all known asymmetries, demonstrates that 2D hydrodynamics explain capsule performance at CR 12 but become less suitable as the CR increases. Mechanisms for this behavior and detailed comparisons of simulations to experiments on NIF will be presented. To evaluate the tradeoff between increased instability and improved 1D performance, we present a full-scale wetted foam capsule design with 17

Exploring the influence of external actors on the cooperation in public–private project organizations for constructing infrastructure

NARCIS (Netherlands)

Koops, L.S.W.; Bosch-Rekveldt, M.G.C.; Bakker, H.L.M.; Hertogh, M.J.C.M.

2017-01-01

Though different forms of public–private partnerships exist, in the organizational structure of most forms a public and a private project organization can be derived, resulting in two collaborating project organizations. The literature on project management however mostly considers one project

Laser coupling to reduced-scale targets at NIF Early Light

International Nuclear Information System (INIS)

Hinkel, D E; Schneider, M B; Young, B K; Holder, J P; Langdon, A B; Baldis, H A; Bonanno, G; Bower, D E; Bruns, H C; Campbell, K M; Celeste, J R; Compton, S; Costa, R L; Dewald, E L; Dixit, S N; Eckart, M J; Eder, D C; Edwards, M J; Ellis, A D; Emig, J A; Froula, D H; Glenzer, S H; Hargrove, D; Haynam, C A; Heeter, R F; Henesian, M A; Holtmeier, G; James, D L; Jancaitis, K S; Kalantar, D H; Kamperschroer, J H; Kauffman, R L; Kimbrough, J; Kirkwood, R K; Koniges, A E; Landen, O L; Landon, M; Lee, F D; MacGowan, B J; Mackinnon, A J; Manes, K R; Marshall, C; May, M J; McDonald, J W; Menapace, J; Moses, S I; Munro, D H; Murray, J R; Niemann, C; Pellinen, D; Power, G D; Rekow, V; Ruppe, J A; Schein, J; Shepherd, R; Singh, M S; Springer, P; Still, C H; Suter, L J; Tietbohl, G L; Turner, R E; VanWonterghem, B M; Wallace, R J; Warrick, A; Watts, P; Weber, F; Wegner, P J; Williams, E A; Young, P E

2005-01-01

Deposition of maximum laser energy into a small, high-Z enclosure in a short laser pulse creates a hot environment. Such targets were recently included in an experimental campaign using the first four of the 192 beams of the National Ignition Facility [J. A. Paisner, E. M. Campbell, and W. J. Hogan, Fusion Technology 26 26, 755 (1994)], under construction at the University of California Lawrence Livermore National Laboratory. These targets demonstrate good laser coupling, reaching a radiation temperature of 340 eV. In addition, the Raman backscatter spectrum contains features consistent with Brillouin backscatter of Raman forward scatter [A. B. Langdon and D. E. Hinkel, Physical Review Letters 89, 015003 (2002)]. Also, NIF Early Light diagnostics indicate that 20% of the direct backscatter from these reduced-scale targets is in the polarization orthogonal to that of the incident light

Culturally appropriate organization of water and sewerage projects built through public private partnerships.

Directory of Open Access Journals (Sweden)

Jessica A Kaminsky

Full Text Available This paper contributes to the pursuit of socially sustainable water and sanitation infrastructure for all people by discovering statistically robust relationships between Hofstede's dimensions of cross-cultural comparison and the choice of contract award types, project type, and primary revenue sources. This analysis, which represents 973 projects distributed across 24 low- and middle-income nations, uses a World Bank dataset describing high capital cost water and sewerage projects funded through private investment. The results show that cultural dimensions explain variation in the choice of contract award types, project type, and primary revenue sources. These results provide empirical evidence that strategies for water and sewerage project organization are not culturally neutral. The data show, for example, that highly individualistic contexts are more likely to select competitive contract award types and to depend on user fees to provide the primary project revenue stream post-construction. By selecting more locally appropriate ways to organize projects, project stakeholders will be better able to pursue the construction of socially sustainable water and sewerage infrastructure.

Culturally appropriate organization of water and sewerage projects built through public private partnerships.

Science.gov (United States)

Kaminsky, Jessica A

2017-01-01

This paper contributes to the pursuit of socially sustainable water and sanitation infrastructure for all people by discovering statistically robust relationships between Hofstede's dimensions of cross-cultural comparison and the choice of contract award types, project type, and primary revenue sources. This analysis, which represents 973 projects distributed across 24 low- and middle-income nations, uses a World Bank dataset describing high capital cost water and sewerage projects funded through private investment. The results show that cultural dimensions explain variation in the choice of contract award types, project type, and primary revenue sources. These results provide empirical evidence that strategies for water and sewerage project organization are not culturally neutral. The data show, for example, that highly individualistic contexts are more likely to select competitive contract award types and to depend on user fees to provide the primary project revenue stream post-construction. By selecting more locally appropriate ways to organize projects, project stakeholders will be better able to pursue the construction of socially sustainable water and sewerage infrastructure.

Organization of spinocerebellar projection map in three types of agranular cerebellum: Purkinje cells vs. granule cells as organizer element

International Nuclear Information System (INIS)

Arsenio Nunes, M.L.; Sotelo, C.; Wehrle, R.

1988-01-01

The organization of the spinocerebellar projection was analysed by the anterograde axonal WGA-HRP (horseradish peroxidase-wheat germ agglutinin conjugate) tracing method in three different types of agranular cerebellar cortex either induced experimentally by X-irradiation or occurring spontaneously in weaver (wv/wv) and staggerer (sg/sg) mutant mice. The results of this study show that in the X-irradiated rat and weaver mouse, in both of which the granule cells are directly affected and die early in development, the spinal axons reproduce, with few differences, the normal spinocerebellar pattern. Conversely, in staggerer mouse, in which the Purkinje cells are intrinsically affected and granule neurons do not seem to be primarily perturbed by the staggerer gene action, the spinocerebellar organization is severely modified. These findings appear somewhat paradoxical because if granule cells, the synaptic targets of mossy spinocerebellar fibers, were necessary for the organization of spinocerebellar projection, the staggerer cerebellum would exhibit a much more normal projectional map than the weaver and the X-irradiated cerebella. It is, therefore, obvious that granule cells, and even specific synaptogenesis, are not essential for the establishment of the normal spinocerebellar topography. On the other hand, the fact that the Purkinje cells are primarily affected in the unique agranular cortex in which the spinocerebellar organization is severely modified suggests that these neurons could be the main element in the organization of the spinocerebellar projection map. This hypothesis is discussed in correlation with already-reported findings on the zonation of the cerebellar cortex by biochemically different clusters of Purkinje cells

A magnetic particle time-of-flight (MagPTOF) diagnostic for measurements of shock- and compression-bang time at the NIF (invited)

Energy Technology Data Exchange (ETDEWEB)

Rinderknecht, H. G., E-mail: hgr@mit.edu; Sio, H.; Frenje, J. A.; Gatu Johnson, M.; Zylstra, A. B.; Sinenian, N.; Rosenberg, M. J.; Li, C. K.; Sèguin, F. H.; Petrasso, R. D. [Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Magoon, J.; Agliata, A.; Shoup, M.; Glebov, V. U.; Hohenberger, M.; Stoeckl, C.; Sangster, T. C. [Laboratory for Laser Energetics, Rochester, New York 14623 (United States); Ayers, S.; Bailey, C. G.; Rygg, J. R. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); and others

2014-11-15

A magnetic particle time-of-flight (MagPTOF) diagnostic has been designed to measure shock- and compression-bang time using D{sup 3}He-fusion protons and DD-fusion neutrons, respectively, at the National Ignition Facility (NIF). This capability, in combination with shock-burn weighted areal density measurements, will significantly constrain the modeling of the implosion dynamics. This design is an upgrade to the existing particle time-of-flight (pTOF) diagnostic, which records bang times using DD or DT neutrons with an accuracy better than ±70 ps [H. G. Rinderknecht et al., Rev. Sci. Instrum. 83, 10D902 (2012)]. The inclusion of a deflecting magnet will increase D{sup 3}He-proton signal-to-background by a factor of 1000, allowing for the first time simultaneous measurements of shock- and compression-bang times in D{sup 3}He-filled surrogate implosions at the NIF.

A magnetic particle time-of-flight (MagPTOF) diagnostic for measurements of shock- and compression-bang time at the NIF (invited).

Science.gov (United States)

Rinderknecht, H G; Sio, H; Frenje, J A; Magoon, J; Agliata, A; Shoup, M; Ayers, S; Bailey, C G; Gatu Johnson, M; Zylstra, A B; Sinenian, N; Rosenberg, M J; Li, C K; Sèguin, F H; Petrasso, R D; Rygg, J R; Kimbrough, J R; Mackinnon, A; Bell, P; Bionta, R; Clancy, T; Zacharias, R; House, A; Döppner, T; Park, H S; LePape, S; Landen, O; Meezan, N; Robey, H; Glebov, V U; Hohenberger, M; Stoeckl, C; Sangster, T C; Li, C; Parat, J; Olson, R; Kline, J; Kilkenny, J

2014-11-01

A magnetic particle time-of-flight (MagPTOF) diagnostic has been designed to measure shock- and compression-bang time using D(3)He-fusion protons and DD-fusion neutrons, respectively, at the National Ignition Facility (NIF). This capability, in combination with shock-burn weighted areal density measurements, will significantly constrain the modeling of the implosion dynamics. This design is an upgrade to the existing particle time-of-flight (pTOF) diagnostic, which records bang times using DD or DT neutrons with an accuracy better than ±70 ps [H. G. Rinderknecht et al., Rev. Sci. Instrum. 83, 10D902 (2012)]. The inclusion of a deflecting magnet will increase D(3)He-proton signal-to-background by a factor of 1000, allowing for the first time simultaneous measurements of shock- and compression-bang times in D(3)He-filled surrogate implosions at the NIF.

Neutron Radiation Shielding For The NIF Streaked X-Ray Detector (SXD) Diagnostic

Energy Technology Data Exchange (ETDEWEB)

Song, P; Holder, J; Young, B; Kalantar, D; Eder, D; Kimbrough, J

2006-11-02

The National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) is preparing for the National Ignition Campaign (NIC) scheduled in 2010. The NIC is comprised of several ''tuning'' physics subcampaigns leading up to a demonstration of Inertial Confinement Fusion (ICF) ignition. In some of these experiments, time-resolved x-ray imaging of the imploding capsule may be required to measure capsule trajectory (shock timing) or x-ray ''bang-time''. A capsule fueled with pure tritium (T) instead of a deutriun-tritium (DT) mixture is thought to offer useful physics surrogacy, with reduced yields of up to 5e14 neutrons. These measurements will require the use of the NIF streak x-ray detector (SXD). The resulting prompt neutron fluence at the planned SXD location ({approx}1.7 m from the target) would be {approx}1.4e9/cm{sup 2}. Previous measurements suggest the onset of significant background at a neutron fluence of {approx} 1e8/cm{sup 2}. The radiation damage and operational upsets which starts at {approx}1e8 rad-Si/sec must be factored into an integrated experimental campaign plan. Monte Carlo analyses were performed to predict the neutron and gamma/x-ray fluences and radiation doses for the proposed diagnostic configuration. A possible shielding configuration is proposed to mitigate radiation effects. The primary component of this shielding is an 80 cm thickness of Polyethylene (PE) between target chamber center (TCC) and the SXD diagnostic. Additionally, 6-8 cm of PE around the detector provide from the large number of neutrons that scatter off the inside of the target chamber. This proposed shielding configuration reduces the high-energy neutron fluence at the SXD by approximately a factor {approx}50.

Neutron Radiation Shielding For The NIF Streaked X-Ray Detector (SXD) Diagnostic

International Nuclear Information System (INIS)

Song, P; Holder, J; Young, B; Kalantar, D; Eder, D; Kimbrough, J

2006-01-01

The National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) is preparing for the National Ignition Campaign (NIC) scheduled in 2010. The NIC is comprised of several ''tuning'' physics subcampaigns leading up to a demonstration of Inertial Confinement Fusion (ICF) ignition. In some of these experiments, time-resolved x-ray imaging of the imploding capsule may be required to measure capsule trajectory (shock timing) or x-ray ''bang-time''. A capsule fueled with pure tritium (T) instead of a deutriun-tritium (DT) mixture is thought to offer useful physics surrogacy, with reduced yields of up to 5e14 neutrons. These measurements will require the use of the NIF streak x-ray detector (SXD). The resulting prompt neutron fluence at the planned SXD location (∼1.7 m from the target) would be ∼1.4e9/cm 2 . Previous measurements suggest the onset of significant background at a neutron fluence of ∼ 1e8/cm 2 . The radiation damage and operational upsets which starts at ∼1e8 rad-Si/sec must be factored into an integrated experimental campaign plan. Monte Carlo analyses were performed to predict the neutron and gamma/x-ray fluences and radiation doses for the proposed diagnostic configuration. A possible shielding configuration is proposed to mitigate radiation effects. The primary component of this shielding is an 80 cm thickness of Polyethylene (PE) between target chamber center (TCC) and the SXD diagnostic. Additionally, 6-8 cm of PE around the detector provide from the large number of neutrons that scatter off the inside of the target chamber. This proposed shielding configuration reduces the high-energy neutron fluence at the SXD by approximately a factor ∼50

Contributions to 30th European Physical Society conference on controlled fusion and plasma physics (St. Petersburg, Russia, 7-11 July 2003) from NIFS

International Nuclear Information System (INIS)

2003-08-01

25 contributed papers to the 30th European Physical Society Conference on Controlled Fusion and Plasma Physics (St. Petersburg, Russia, 7-11 July 2003) from the activity of NIFS are collected in this report. (author)

Balancing management ‘for’ and ‘of’ stakeholders in projects and in temporary organizations

DEFF Research Database (Denmark)

Aagaard, Annabeth; Eskerod, Pernille; Huemann, Martina

need stakeholder resources, e.g. expertise and raw materials, and how this is managed appears to have an impact on the success of the project. This paper addressed the question on how sustainable development can be enhanced by building bridge between two fundamentally different management approaches......The global developments within society have an impact on all organizations and therefore also on temporary organizations, like projects. However, these temporary organizations are often managed differently than permanent organizations, as projects are more fluid and time restricted. Yet, they still...... to stakeholders: management ‘of’ versus ‘for’ stakeholders. The former derives from strategic management, ‘how do we make stakeholders comply with the organization’s needs?’, whereas the latter derives from business ethics, ‘how do we accommodate the needs of the stakeholders?’. A conceptual framework pointing...

Project based learning in organizations: towards a methodology for learning in groups

NARCIS (Netherlands)

Poell, R.F.; Krogt, F.J. van der

2003-01-01

This article introduces a methodology for employees in organizations to set up and carry out their own group learning projects. It is argued that employees can use project-based learning to make their everyday learning more systematic at times, without necessarily formalizing it. The article

Project-based learning in organizations : Towards a methodology for learning in groups

NARCIS (Netherlands)

Poell, R.F.; van der Krogt, F.J.

2003-01-01

This article introduces a methodology for employees in organizations to set up and carry out their own group learning projects. It is argued that employees can use project-based learning to make their everyday learning more systematic at times, without necessarily formalizing it. The article

NIF laser bundle review. Final report

International Nuclear Information System (INIS)

Tietbohl, G.L.; Larson, D.W.; Erlandson, A.C.

1995-01-01

We performed additional bundle review effort subsequent to the completion of the preliminary report and are revising our original recommendations. We now recommend that the NIF baseline laser bundle size be changed to the 4x2 bundle configuration. There are several 4x2 bundle configurations that could be constructed at a cost similar to that of the baseline 4x12 (from $11M more to about $11M less than the baseline; unescalated, no contingency) and provide significant system improvements. We recommend that the building cost estimates (particularly for the in-line building options) be verified by an architect/engineer (A/E) firm knowledgeable about building design. If our cost estimates of the in-line building are accurate and therefore result in a change from the baseline U-shaped building layout, the acceptability of the in-line configuration must be reviewed from an operations viewpoint. We recommend that installation, operation, and maintenance of all laser components be reviewed to better determine the necessity of aisles, which add to the building cost significantly. The need for beam expansion must also be determined since it affects the type of bundle packing that can be used and increases the minimum laser bay width. The U-turn laser architecture (if proven viable) offers a reduction in building costs since this laser design is shorter than the baseline switched design and requires a shorter laser bay

Short pulse, high resolution, backlighters for point projection high-energy radiography at the National Ignition Facility

Science.gov (United States)

Tommasini, R.; Bailey, C.; Bradley, D. K.; Bowers, M.; Chen, H.; Di Nicola, J. M.; Di Nicola, P.; Gururangan, G.; Hall, G. N.; Hardy, C. M.; Hargrove, D.; Hermann, M.; Hohenberger, M.; Holder, J. P.; Hsing, W.; Izumi, N.; Kalantar, D.; Khan, S.; Kroll, J.; Landen, O. L.; Lawson, J.; Martinez, D.; Masters, N.; Nafziger, J. R.; Nagel, S. R.; Nikroo, A.; Okui, J.; Palmer, D.; Sigurdsson, R.; Vonhof, S.; Wallace, R. J.; Zobrist, T.

2017-05-01

High-resolution, high-energy X-ray backlighters are very active area of research for radiography experiments at the National Ignition Facility (NIF) [Miller et al., Nucl. Fusion 44, S228 (2004)], in particular those aiming at obtaining Compton-scattering produced radiographs from the cold, dense fuel surrounding the hot spot. We report on experiments to generate and characterize point-projection-geometry backlighters using short pulses from the advanced radiographic capability (ARC) [Crane et al., J. Phys. 244, 032003 (2010); Di Nicola et al., Proc. SPIE 2015, 93450I-12], at the NIF, focused on Au micro-wires. We show the first hard X-ray radiographs, at photon energies exceeding 60 keV, of static objects obtained with 30 ps-long ARC laser pulses, and the measurements of strength of the X-ray emission, the pulse duration and the source size of the Au micro-wire backlighters. For the latter, a novel technique has been developed and successfully applied.

NIFS joint research meeting on plasma facing components, PSI, and heat/particle control

International Nuclear Information System (INIS)

Yamashina, T.

1997-10-01

The LHD collaboration has been started in 1996. Particle and heat control is one of the categories for the collaboration, and a few programs have been nominated in these two years. A joint research meeting on PFC, PSI, heat and particle meeting was held at NIFS on June 27, 1997, in which present status of these programs were reported. This is a collection of the notes and view graphs presented in this meeting. Brief reviews and research plan of each program are included in relation to divertor erosion and sputtering, impurity generation, hydrogen recycling, edge plasma structure, edge transport and its control, heat removal, particle exhaust, wall conditioning etc. (author)

Organizational Performance: Integration of the Value, Rarity, Imitability and Organization Framework in Project Management

Directory of Open Access Journals (Sweden)

Ronielton Rezende Oliveira

2017-08-01

Full Text Available Organizational performance is often associated with competitive advantage. In strategy studie saimed at expanding communication on a global vision of organizations and their initiatives, strategic project management stands out, whichis related to the mechanisms and set of management decisions that determine long term organizational performance. This study verify how the Resource-based view adds value to organizational initiatives seeking to achieve organizational performance through projects. The study considers strategic management practiced by the project managers o asto present a conceptual framework with four propositions that demonstrate the integration of the Value, Rarity, Imitability and Organization framework with project management and its relations to organizational performance.

Case Writing Projects in Co-Operation with Companies and Organizations

OpenAIRE

Bengtsson, Lars; Asplund, Carl-Johan

2008-01-01

The purpose of this paper is to present the process and evaluation of case writing projects in co-operation with companies and organizations in a course for engineering students. The case writing projects could provide an illustration and example framework for working with companies in constructing cases. Normally cases are constructed for teaching purposes in higher education. However, in order to get closer co-operation and more interest from the companies the authors encouraged the student...

Integrated computer control system CORBA-based simulator FY98 LDRD project final summary report

International Nuclear Information System (INIS)

Bryant, R M; Holloway, F W; Van Arsdall, P J.

1999-01-01

The CORBA-based Simulator was a Laboratory Directed Research and Development (LDRD) project that applied simulation techniques to explore critical questions about distributed control architecture. The simulator project used a three-prong approach comprised of a study of object-oriented distribution tools, computer network modeling, and simulation of key control system scenarios. This summary report highlights the findings of the team and provides the architectural context of the study. For the last several years LLNL has been developing the Integrated Computer Control System (ICCS), which is an abstract object-oriented software framework for constructing distributed systems. The framework is capable of implementing large event-driven control systems for mission-critical facilities such as the National Ignition Facility (NIF). Tools developed in this project were applied to the NIF example architecture in order to gain experience with a complex system and derive immediate benefits from this LDRD. The ICCS integrates data acquisition and control hardware with a supervisory system, and reduces the amount of new coding and testing necessary by providing prebuilt components that can be reused and extended to accommodate specific additional requirements. The framework integrates control point hardware with a supervisory system by providing the services needed for distributed control such as database persistence, system start-up and configuration, graphical user interface, status monitoring, event logging, scripting language, alert management, and access control. The design is interoperable among computers of different kinds and provides plug-in software connections by leveraging a common object request brokering architecture (CORBA) to transparently distribute software objects across the network of computers. Because object broker distribution applied to control systems is relatively new and its inherent performance is roughly threefold less than traditional point

Seminar on the organization and management of a commercial nuclear power project

International Nuclear Information System (INIS)

Peddicord, K.L.; Alvis, J.M.; Chitkara, K.K.

1986-01-01

A main function of student branches of the American Nuclear Society (ANS) is to identify activities that contribute to the professional development and growth of its student members. Over the past several years, the ANS student branch at Texas A and M University has toured the construction site of the South Texas Nuclear Project (STNP), which is jointly owned by the Houston Lighting and Power Company (HL and P), the City of San Antonio, the Central Power and Light Company, and the City of Austin. This year, student branch organizers recognized that another aspect of a commercial nuclear power plant was not being covered either in the academic course work or the plant tours. This facet includes the organization and management required to undertake a major nuclear power project. To fill this gap, HL and P sponsored a one-day seminar that covered the various managerial functions for STNP. The seminar on the Organization and Management at a Commercial Nuclear Power Project was very interesting and beneficial. Other ANS branches and utilities may find this to be a useful model for future activities

NiF2/NaF:CaF2/Ca Solid-State High-Temperature Battery Cells

Science.gov (United States)

West, William; Whitacre, Jay; DelCastillo, Linda

2009-01-01

Experiments and theoretical study have demonstrated the promise of all-solid-state, high-temperature electrochemical battery cells based on NiF2 as the active cathode material, CaF2 doped with NaF as the electrolyte material, and Ca as the active anode material. These and other all-solid-state cells have been investigated in a continuing effort to develop batteries for instruments that must operate in environments much hotter than can be withstood by ordinary commercially available batteries. Batteries of this type are needed for exploration of Venus (where the mean surface temperature is about 450 C), and could be used on Earth for such applications as measuring physical and chemical conditions in geothermal wells and oil wells. All-solid-state high-temperature power cells are sought as alternatives to other high-temperature power cells based, variously, on molten anodes and cathodes or molten eutectic salt electrolytes. Among the all-solid-state predecessors of the present NiF2/NaF:CaF2/Ca cells are those described in "Solid-State High-Temperature Power Cells" (NPO-44396), NASA Tech Briefs, Vol. 32, No. 5 (May 2008), page 40. In those cells, the active cathode material is FeS2, the electrolyte material is a crystalline solid solution of equimolar amounts of Li3PO4 and LiSiO4, and the active anode material is Li contained within an alloy that remains solid in the intended high operational temperature range.

Recent developments in the structural organization and regulation of nitrogen fixation genes in Herbaspirillum seropedicae.

Science.gov (United States)

Pedrosa, F O; Benelli, E M; Yates, M G; Wassem, R; Monteiro, R A; Klassen, G; Steffens, M B; Souza, E M; Chubatsu, L S; Rigo, L U

2001-10-04

Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrogen fixation bacteria. Nitrogen fixation control in H. seropedicae, of the beta-subgroup of Proteobacteria, has regulatory features in common with Klebsiella pneumoniae, of the gamma-subgroup, at the level of nifA expression and with rhizobia and Azospirillum brasilense, of the alpha-subgroup, at the level of control of NifA by oxygen.

Damage sources for the NIF Grating Debris Shield (GDS) and methods for their mitigation

Science.gov (United States)

Carr, C. W.; Bude, J.; Miller, P. E.; Parham, T.; Whitman, P.; Monticelli, M.; Raman, R.; Cross, D.; Welday, B.; Ravizza, F.; Suratwala, T.; Davis, J.; Fischer, M.; Hawley, R.; Lee, H.; Matthews, M.; Norton, M.; Nostrand, M.; Vanblarcom, D.; Sommer, S.

2017-11-01

The primary sources of damage on the National Ignition Facility (NIF) Grating Debris Shield (GDS) are attributed to two independent types of laser-induced particulates. The first comes from the eruptions of bulk damage in a disposable debris shield downstream of the GDS. The second particle source comes from stray light focusing on absorbing glass armor at higher than expected fluences. We show that the composition of the particles is secondary to the energetics of their delivery, such that particles from either source are essentially benign if they arrive at the GDS with low temperatures and velocities.

Technology projects for characterization--monitoring of volatile organic compounds (VOCs)

Energy Technology Data Exchange (ETDEWEB)

Junk, G.A.; Haas, W.J. Jr.

1992-07-01

One hundred thirty technology project titles related to the characterization of volatile organic compounds (VOCs) at an arid site are listed alphabetically by first contact person in a master compilation that includes phone numbers, addresses, keywords, and short descriptions. Separate tables are presented for 62 field-demonstrated, 36 laboratory-demonstrated, and 35 developing technology projects. The technology projects in each of these three categories are also prioritized in separate summary tables. Additional tables are presented for a number of other categorizations of the technology projects: In Situ; Fiberoptic; Mass Spectrometer; Optical Spectroscopy; Raman or SERS; Ion Mobility or Acoustic; Associated; and Commercial. Four lists of contact person names are provided so details concerning the projects that deal with sampling, and VOCs in gases, waters, and soils (sediments) can be obtained. Finally, seven wide-ranging conclusions based on observations and experiences during this work are presented.

Technology projects for characterization--monitoring of volatile organic compounds (VOCs)

International Nuclear Information System (INIS)

Junk, G.A.; Haas, W.J. Jr.

1992-07-01

One hundred thirty technology project titles related to the characterization of volatile organic compounds (VOCs) at an arid site are listed alphabetically by first contact person in a master compilation that includes phone numbers, addresses, keywords, and short descriptions. Separate tables are presented for 62 field-demonstrated, 36 laboratory-demonstrated, and 35 developing technology projects. The technology projects in each of these three categories are also prioritized in separate summary tables. Additional tables are presented for a number of other categorizations of the technology projects: In Situ; Fiberoptic; Mass Spectrometer; Optical Spectroscopy; Raman or SERS; Ion Mobility or Acoustic; Associated; and Commercial. Four lists of contact person names are provided so details concerning the projects that deal with sampling, and VOCs in gases, waters, and soils (sediments) can be obtained. Finally, seven wide-ranging conclusions based on observations and experiences during this work are presented

Acoustic Longitudinal Field NIF Optic Feature Detection Map Using Time-Reversal & MUSIC

Energy Technology Data Exchange (ETDEWEB)

Lehman, S K

2006-02-09

We developed an ultrasonic longitudinal field time-reversal and MUltiple SIgnal Classification (MUSIC) based detection algorithm for identifying and mapping flaws in fused silica NIF optics. The algorithm requires a fully multistatic data set, that is one with multiple, independently operated, spatially diverse transducers, each transmitter of which, in succession, launches a pulse into the optic and the scattered signal measured and recorded at every receiver. We have successfully localized engineered ''defects'' larger than 1 mm in an optic. We confirmed detection and localization of 3 mm and 5 mm features in experimental data, and a 0.5 mm in simulated data with sufficiently high signal-to-noise ratio. We present the theory, experimental results, and simulated results.

Analysis of BigFoot HDC SymCap experiment N161205 on NIF

Science.gov (United States)

Dittrich, T. R.; Baker, K. L.; Thomas, C. A.; Berzak Hopkins, L. F.; Harte, J. A.; Zimmerman, G. B.; Woods, D. T.; Kritcher, A. L.; Ho, D. D.; Weber, C. R.; Kyrala, G.

2017-10-01

Analysis of NIF implosion experiment N161205 provides insight into both hohlraum and capsule performance. This experiment used an undoped High Density Carbon (HDC) ablator driven by a BigFoot x-ray profile in a Au hohlraum. Observations from this experiment include DT fusion yield, bang time, DSR, Tion and time-resolved x-ray emission images around bang time. These observations are all consistent with an x-ray spectrum having significantly reduced Au m-band emission that is present in a standard hohlraum simulation. Attempts to justify the observations using several other simulation modifications will be presented. This work was performed under the auspices of the Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344.

3D studies of the NIF symmetry tuning targets

Science.gov (United States)

Milovich, J.; Jones, O.; Edwards, M.; Weber, S.; Dewald, E.; Landen, O.; Marinak, M.

2009-11-01

Minimizing radiation drive asymmetries is necessary for a successful ignition campaign. Since the ignition capsule symmetry is most sensitive to the foot (first 2 ns) and the peak of the laser pulse, two different targets will be fielded on the NIF: re-emit and symmetry capsules (Sym-Caps). The first measures the incoming flux asymmetries during the foot by observing the re-radiated flux of a high-Z ball in place of the ignition capsule. The Sym-Caps resemble the ignition target with the frozen DT layer replaced by an equivalent mass of ablator material, thus preserving the hydrodynamic implosion properties. By measuring the x-ray self-emission near peak compression the ignition capsule core shape can be tuned. Simulations with 2D radiation-hydrodynamic simulations codes omit 3D effects in the hohlraum such as diagnostic holes, capsule roughness, shot-to-shot variations caused by laser beam power imbalances and pointing errors. We study these effects by performing 3D simulations using HYDRA and found that tuning the laser pulse using a finite number of shots is not substantially compromised.

The Ignition Physics Campaign on NIF: Status and Progress

International Nuclear Information System (INIS)

Edwards, M. J.

2016-01-01

We have made significant progress in ICF implosion performance on NIF since the 2011 IFSA. Employing a 3-shock, high adiabat CH (“High-Foot”) design, total neutron yields have increased 10-fold to 6.3 x10 15 (a yield of ∼ 17 kJ, which is greater than the energy invested in the DT fuel ∼ 12kJ). At that level, the yield from alpha self-heating is essentially equivalent to the compression yield, indicating that we are close to the alpha self-heating regime. Low adiabat, 4-shock High Density Carbon (HDC) capsules have been imploded in conventional gas-filled hohlraums, and employing a 6 ns, 2-shock pulse, HDC capsules were imploded in near-vacuum hohlraums with overall coupling ∼ 98%. Both the 4- and 2-shock HDC capsules had very low mix and high yield over simulated performance. Rugby holraums have demonstrated uniform x-ray drive with minimal Cross Beam Energy Transfer (CBET), and we have made good progress in measuring and modelling growth of ablation front hydro instabilities. (paper)

Progress towards monochromatic imaging of mix at the NIF

Science.gov (United States)

Kyrala, G. A.; Murphy, T. J.; Bradley, P. A.; Krashenninnikova, N. S.; Tregillis, I. L.; Obrey, K.; Shah, R. C.; Hakel, P.; Kline, J. L.; Grim, G. P.; Schmitt, M. J.; Kanzleiter, R. J.; Regan, S. P.; Barrios, M. A.

2013-10-01

Mix of non-hydrogenic (Z >1) material into the hydrogenic (D and T) ICF capsule fuel degrades implosion performance. The amount of degradation depends on the degree and the spatial distribution of mix. Experiments are underway at NIF to quantify the mix of shell material into fuel using directly driven capsules. CH or CD shells with various dopants, implanted at different depths in the shell are being used to change the amount of dopant mix. Spatially and spectrally resolved emission from the ionized dopants will be used to generate spatially and temporally dependent density and temperature maps of the ionized dopants that are mixed and heated in the core plasma. This information will be used to validate different mix models. This talk will describe the search for the appropriate dopant that gave a radiation spectrum that could be used to record images with the MMI diagnostic. This work is supported by US DOE/NNSA, performed at LANL, operated by LANS LLC under contract DE-AC52-06NA25396.

Rethinking project portfolio management: A case study of a public organization

DEFF Research Database (Denmark)

Hansen, Lars Kristian; Svejvig, Per

2018-01-01

analytical perspectives. One perspective, denoted as classical project management (CPM), highlights key characteristics of traditional PPM research. A second perspective, denoted as rethinking project management (RPM), highlights characteristics of progressive PPM research. Against this background, we...... diagnose PPM practices in a large public organization and find that organizational top management use the vocabulary and request the values emphasized by the CPM perspective, such as executability, controllability, instrumentality and linearity. However, below top management level, we find managers...... practices. Taking into account the limitations of our single case study design, our research shows how an organization, which differs considerably from advise provided by the conventional CPM perspective, and best PPM practices, may be well functioning – or at least, in large, meets the organization’ PPM...

Using deep neural networks to augment NIF post-shot analysis

Science.gov (United States)

Humbird, Kelli; Peterson, Luc; McClarren, Ryan; Field, John; Gaffney, Jim; Kruse, Michael; Nora, Ryan; Spears, Brian

2017-10-01

Post-shot analysis of National Ignition Facility (NIF) experiments is the process of determining which simulation inputs yield results consistent with experimental observations. This analysis is typically accomplished by running suites of manually adjusted simulations, or Monte Carlo sampling surrogate models that approximate the response surfaces of the physics code. These approaches are expensive and often find simulations that match only a small subset of observables simultaneously. We demonstrate an alternative method for performing post-shot analysis using inverse models, which map directly from experimental observables to simulation inputs with quantified uncertainties. The models are created using a novel machine learning algorithm which automates the construction and initialization of deep neural networks to optimize predictive accuracy. We show how these neural networks, trained on large databases of post-shot simulations, can rigorously quantify the agreement between simulation and experiment. This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Washoe Tribe Nevada Inter-Tribal Energy Consortium Energy Organization Enhancement Project Final Report

Energy Technology Data Exchange (ETDEWEB)

Johnson, Jennifer [Washoe Tribe of NV and Ca

2014-11-06

The Washoe Tribe of Nevada and California was awarded funding from the Department of Energy to complete the Nevada Inter-Tribal Energy Consortium Energy Organization Enhancement Project. The main goal of the project was to enhance the capacity of the Nevada Inter-Tribal Energy Consortium (NITEC) to effectively assist tribes within Nevada to technically manage tribal energy resources and implement tribal energy projects.

Overview of the gamma reaction history diagnostic for the national ignition facility (NIF)

International Nuclear Information System (INIS)

Kim, Yong Ho; Evans, Scott C.; Herrmann, Hans W.; Mack, Joseph M.; Young, Carl S.; Malone, Robert M.; Cox, Brian C.; Frogget, Brent C.; Kaufman, Morris I.; Tunnell, Thomas W.; Tibbitts, Aric; Palagi, Martin J.; Stoeffl, Wolfgang

2010-01-01

The National Ignition Facility (NIF) has a need for measuring gamma radiation as part of a nuclear diagnostic program. A new gamma-detection diagnostic uses 900 off-axis parabolic mirrors to rel ay Cherenkov light from a volume of pressurized gas. This non imaging optical system has the high-speed detector placed at a stop position with the Cherenkov light delayed until after the prompt gammas have passed through the detector. Because of the wavelength range (250 to 700 nm), the optical element surface finish was a key design constraint. A cluster of four channels (each set to a different gas pressure) will collect the time histories for different energy ranges of gammas.

Hohlraum glint and laser pre-pulse detector for NIF experiments using velocity interferometer system for any reflector.

Science.gov (United States)

Moody, J D; Clancy, T J; Frieders, G; Celliers, P M; Ralph, J; Turnbull, D P

2014-11-01

Laser pre-pulse and early-time laser reflection from the hohlraum wall onto the capsule (termed "glint") can cause capsule imprint and unwanted early-time shocks on indirect drive implosion experiments. In a minor modification to the existing velocity interferometer system for any reflector diagnostic on NIF a fast-response vacuum photodiode was added to detect this light. The measurements show evidence of laser pre-pulse and possible light reflection off the hohlraum wall and onto the capsule.

Auxins upregulate nif and fix genes.

Science.gov (United States)

Bianco, Carmen; Defez, Roberto

2010-10-01

In a recent publication we analyzed the global effects triggered by IAA overproduction in S. meliloti RD64 under free-living conditions by comparing the gene expression pattern of wild type 1021 with that of RD64 and 1021 treated with IAA and other four chemically or functionally related molecules. Among the genes differentially expressed in RD64 and IAA-treated 1021 cells we found two genes of pho operon, phoT and phoC. Based on this finding we examined the mechanisms for mineral P solubilization in RD64 and the potential ability of this strain to improve Medicago growth under P-starved conditions. Here, we further analyze the expression profiles obtained in microarray analysis and evaluate the specificity and the extent of overlap between all treatments. Venn diagrams indicated that IAA- and 2,4-D-regulated genes were closely related. Furthermore, most differentially expressed genes from pSymA were induced in 1021 cells treated with 2,4-D, ICA, IND and Trp as compared to the untreated 1021 cells. RT-PCR analysis was employed to analyze the differential expression patterns of nitrogen fixation genes under free-living and symbiotic conditions. Under symbiotic condition, the relative expression levels of nif and fix genes were significantly induced in Mt- RD64 plants and in Mt-1021 plants treated with IAA and 2,4-D whereas they were unchanged or repressed in Mt-1021 plants treated with the other selected compounds when compared to the untreated Mt-1021 plants. © 2010 Landes Bioscience

The coincidence counting technique for orders of magnitude background reduction in data obtained with the magnetic recoil spectrometer at OMEGA and the NIF

International Nuclear Information System (INIS)

Casey, D. T.; Frenje, J. A.; Seguin, F. H.; Li, C. K.; Rosenberg, M. J.; Rinderknecht, H.; Manuel, M. J.-E.; Gatu Johnson, M.; Schaeffer, J. C.; Frankel, R.; Sinenian, N.; Childs, R. A.; Petrasso, R. D.; Glebov, V. Yu.; Sangster, T. C.; Burke, M.; Roberts, S.

2011-01-01

A magnetic recoil spectrometer (MRS) has been built and successfully used at OMEGA for measurements of down-scattered neutrons (DS-n), from which an areal density in both warm-capsule and cryogenic-DT implosions have been inferred. Another MRS is currently being commissioned on the National Ignition Facility (NIF) for diagnosing low-yield tritium-hydrogen-deuterium implosions and high-yield DT implosions. As CR-39 detectors are used in the MRS, the principal sources of background are neutron-induced tracks and intrinsic tracks (defects in the CR-39). The coincidence counting technique was developed to reduce these types of background tracks to the required level for the DS-n measurements at OMEGA and the NIF. Using this technique, it has been demonstrated that the number of background tracks is reduced by a couple of orders of magnitude, which exceeds the requirement for the DS-n measurements at both facilities.

The coincidence counting technique for orders of magnitude background reduction in data obtained with the magnetic recoil spectrometer at OMEGA and the NIF.

Science.gov (United States)

Casey, D T; Frenje, J A; Séguin, F H; Li, C K; Rosenberg, M J; Rinderknecht, H; Manuel, M J-E; Gatu Johnson, M; Schaeffer, J C; Frankel, R; Sinenian, N; Childs, R A; Petrasso, R D; Glebov, V Yu; Sangster, T C; Burke, M; Roberts, S

2011-07-01

A magnetic recoil spectrometer (MRS) has been built and successfully used at OMEGA for measurements of down-scattered neutrons (DS-n), from which an areal density in both warm-capsule and cryogenic-DT implosions have been inferred. Another MRS is currently being commissioned on the National Ignition Facility (NIF) for diagnosing low-yield tritium-hydrogen-deuterium implosions and high-yield DT implosions. As CR-39 detectors are used in the MRS, the principal sources of background are neutron-induced tracks and intrinsic tracks (defects in the CR-39). The coincidence counting technique was developed to reduce these types of background tracks to the required level for the DS-n measurements at OMEGA and the NIF. Using this technique, it has been demonstrated that the number of background tracks is reduced by a couple of orders of magnitude, which exceeds the requirement for the DS-n measurements at both facilities.

Projections in donor organs available for liver transplantation in the United States: 2014-2025.

Science.gov (United States)

Parikh, Neehar D; Hutton, David; Marrero, Wesley; Sanghani, Kunal; Xu, Yongcai; Lavieri, Mariel

2015-06-01

With the aging US population, demographic shifts, and obesity epidemic, there is potential for further exacerbation of the current liver donor shortage. We aimed to project the availability of liver grafts in the United States. We performed a secondary analysis of the Organ Procurement and Transplantation Network database of all adult donors from 2000 to 2012 and calculated the total number of donors available and transplanted donor livers stratified by age, race, and body mass index (BMI) group per year. We used National Health and Nutrition Examination Survey and Centers for Disease Control and Prevention historical data to stratify the general population by age, sex, race, and BMI. We then used US population age and race projections provided by the US Census Bureau and the Weldon Cooper Center for Public Service and made national and regional projections of available donors and donor liver utilization from 2014 to 2025. We performed sensitivity analyses and varied the rate of the rise in obesity, proportion of Hispanics, population growth, liver utilization rate, and donation after cardiac death (DCD) utilization. The projected adult population growth in the United States from 2014 to 2025 will be 7.1%. However, we project that there will be a 6.1% increase in the number of used liver grafts. There is marked regional heterogeneity in liver donor growth. Projections were significantly affected by changes in BMI, DCD utilization, and liver utilization rates but not by changes in the Hispanic proportion of the US population or changes in the overall population growth. Overall population growth will outpace the growth of available donor organs and thus potentially exacerbate the existing liver graft shortage. The projected growth in organs is highly heterogeneous across different United Network for Organ Sharing regions. Focused strategies to increase the liver donor pool are warranted. © 2015 American Association for the Study of Liver Diseases.

Team effectiveness in Non-Governmental Organizations (NGOs) projects.

Science.gov (United States)

Latif, Khawaja Fawad; Williams, Nigel

2017-10-01

The incorporation of team context into research and practice regarding team effectiveness in NGOs projects is a constant challenge. The research seeks to address the gap and identify the critical determinants of team effectiveness in projects undertaken by non-governmental organizations. Using a systematic process, the study involved both literature and focus group discussions to generate the required items. A total of 157 respondents (Team Members and Team Leaders) were part of the study that filled the questionnaires. Using exploratory factor analysis followed by confirmatory factor analysis, both convergent and discriminant validity was established. The present study found that team effectiveness in NGO social projects has a total of seven dimensions namely: Inter team coordination, community social linkage, team performance, knowledge, skills, and attitudes, leadership communication and engagement, decision making and information sharing, and team formation. There is a significant lack of research on team effectiveness in NGO projects. Where considerably large proportion of research on team effectiveness has focused on the corporate sector, the non-governmental teams have been neglected. This study clearly highlights the determinants that make up team effectiveness in NGOs. The determinants identified will help to specifically look at the effectiveness of teams in NGO projects. The study would help NGOs identify the dimensions in which they may be performing in a weaker manner and direct their energies in improving the factors. Copyright © 2017 Elsevier Ltd. All rights reserved.

Online Tutoring Procedure for Research Project Supervision: Management, Organization and Key Elements

Science.gov (United States)

Darder Mesquida, Antònia; Pérez Garcias, Adolfina

2015-01-01

Research project tutoring appears as a crucial element for teaching; it is a planned action based on the relationship between a tutor and a student. This paper presents the findings of a design and development research which has as its main aim to create an organization system for the tutoring of online research projects. That system seeks to…

Posttranslational modification of Klebsiella pneumoniae flavodoxin by covalent attachment of coenzyme A, shown by sup 31 P NMR and electrospray mass spectrometry, prevents electron transfer from the nifJ protein to nitrogenase. A possible new regulatory mechanism for biological nitrogen fixation

Energy Technology Data Exchange (ETDEWEB)

Thorneley, R.N.F.; Ashby, G.A.; Drummond, M.H.; Eady, R.R.; Huff, S.; Macdonald, C.J. (Univ. of Sussex, Brighton (United Kingdom)); Abell, C.; Schneier, A. (Univ. Chemical Lab., Cambridge (United Kingdom))

1992-02-04

A strain of Escherichia coli (71-18) that produces ca. 15% of its soluble cytoplasmic protein as a flavodoxin, the Klebsiella pneumoniae nifF gene product, has been constructed. The flavodoxin was purified using FPLC and resolved into two forms, designated KpFldI and KpFldII, which were shown to have identical N-terminal amino acid sequences (30 residues) in agreement with that predicted by the K. pneumoniae nifF DNA sequence. {sup 31}P NMR, electrospray mass spectrometry, UV-visible spectra, and thiol group estimations showed that the single cysteine residue (position 68) of KpFldI is posttranslationally modified in KpFldII by the covalent, mixed disulfide, attachment of coenzyme A. KpFldII was inactive as an electron carrier between the K. pneumoniae nifJ product (a pyruvate-flavodoxin oxidoreductase) and K. pneumoniae nifH product (the Fe-protein of nitrogenase). This novel posttranslational modification of a flavodoxin is discussed in terms of the regulation of nitrogenase activity in vivo in response to the level of dissolved O{sub 2} and the carbon status of diazotrophic cultures.

Human resource management in the project-oriented organization: Employee well-being and ethical treatment

OpenAIRE

Turner, R.; Huemann, M.; Keegan, A.

2008-01-01

As part of a wider study into human resource management (HRM) practices in project-oriented organizations, we investigated the issue of employee well-being. Project-oriented organizations adopt temporary work processes to deliver products and services to clients. This creates a dynamic work environment, where additional pressures can be imposed on the employee from fluctuating work-loads, uncertain requirements, and multiple role demands. These pressures can create issues for employee well-be...

Spectroscopic diagnostics of NIF ICF implosions using line ratios of Kr dopant in the ignition capsule

Science.gov (United States)

Dasgupta, Arati; Ouart, Nicholas; Giuiani, John; Clark, Robert; Schneider, Marilyn; Scott, Howard; Chen, Hui; Ma, Tammy

2017-10-01

X ray spectroscopy is used on the NIF to diagnose the plasma conditions in the ignition target in indirect drive ICF implosions. A platform is being developed at NIF where small traces of krypton are used as a dopant to the fuel gas for spectroscopic diagnostics using krypton line emissions. The fraction of krypton dopant was varied in the experiments and was selected so as not to perturb the implosion. Our goal is to use X-ray spectroscopy of dopant line ratios produced by the hot core that can provide a precise measurement of electron temperature. Simulations of the krypton spectra using a 1 in 104 atomic fraction of krypton in direct-drive exploding pusher with a range of electron temperatures and densities show discrepancies when different atomic models are used. We use our non-LTE atomic model with a detailed fine-structure level atomic structure and collisional-radiative rates to investigate the krypton spectra at the same conditions. Synthetic spectra are generated with a detailed multi-frequency radiation transport scheme from the emission regions of interest to analyze the experimental data with 0.02% Kr concentration and compare and contrast with the existing simulations at LLNL. Work supported by DOE/NNSA; Part of this work was also done under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344.

Experimental study on the resistance to hydrogen embrittlement of NIFS-V4Cr4Ti alloy

International Nuclear Information System (INIS)

Chen Jiming; Xu Zengyu; Den Ying; Muroga, T.

2002-01-01

SWIP (Southwestern Institute of Physics) has joined an international collaboration on the hydrogen embrittlement resistance evaluation of the vanadium alloy. This paper presents some experiments on the tensile properties and Charpy impact properties of the NIFS-V4Cr4Ti alloy with high-level hydrogen concentration. The experiment results show different properties against hydrogen embrittlement in static tension and impact load. The critical hydrogen concentration required to embrittle the alloy was about 215 - 310 mg·kg -1 on static tension load, but less than 130 mg·kg -1 on impact loading

Exploring the universe through Discovery Science on NIF

Science.gov (United States)

Remington, Bruce

2017-10-01

New regimes of science are being experimentally studied at high energy density facilities around the world, spanning drive energies from microjoules to megajoules, and time scales from femtoseconds to microseconds. The ability to shock and ramp compress samples to very high pressures and densities allows new states of matter relevant to planetary and stellar interiors to be studied. Shock driven hydrodynamic instabilities evolving into turbulent flows relevant to the dynamics of exploding stars (such as supernovae), accreting compact objects (such as white dwarfs, neutron stars, and black holes), and planetary formation dynamics (relevant to the exoplanets) are being probed. The dynamics of magnetized plasmas relevant to astrophysics, both in collisional and collisionless systems, are starting to be studied. High temperature, high velocity interacting flows are being probed for evidence of astrophysical collisionless shock formation, the turbulent magnetic dynamo effect, magnetic reconnection, and particle acceleration. And new results from thermonuclear reactions in hot dense plasmas relevant to stellar and big bang nucleosynthesis are starting to emerge. A selection of examples of frontier research through NIF Discovery Science in the coming decade will be presented. This work was performed under the auspices of U.S. DOE by LLNL under Contract DE-AC52-07NA27344.

Temporal multiplexing for economical measurement of power versus time on NIF

International Nuclear Information System (INIS)

Thomas, S.; Boyd, B.; Davis, D.T.; Hall, B.

1996-10-01

The researchers have designed an economical device to measure the power time history in the National Ignition Facility's (NIF) 192 beam laser. The heart of the system is a commercial, high-speed, four-channel digitizer with a 15,000 point record length. Samples of several beams are taken with fiberoptic pickoffs, separated in time with appropriate fiberoptic delays and presented to high-speed vacuum photodiodes, which convert the samples to electrical signals for the digitizer. Amplitude and time multiplexing are used to cover the required dynamic range and to record 12 samples on the digitizer, making the cost per sample competitive with alternative approaches. Forty-eight digitizers can record the required three samples from each of the 192 beams. An additional similar but lower bandwidth system is used to record the backreflected light from the main laser amplifiers and elsewhere. The recording electronics are discussed in detail

Temporal multiplexing for economical measurement of power versus time on NIF

Energy Technology Data Exchange (ETDEWEB)

Thomas, S.; Boyd, B.; Davis, D.T.; Hall, B.

1996-10-01

The researchers have designed an economical device to measure the power time history in the National Ignition Facility`s (NIF) 192 beam laser. The heart of the system is a commercial, high-speed, four-channel digitizer with a 15,000 point record length. Samples of several beams are taken with fiberoptic pickoffs, separated in time with appropriate fiberoptic delays and presented to high-speed vacuum photodiodes, which convert the samples to electrical signals for the digitizer. Amplitude and time multiplexing are used to cover the required dynamic range and to record 12 samples on the digitizer, making the cost per sample competitive with alternative approaches. Forty-eight digitizers can record the required three samples from each of the 192 beams. An additional similar but lower bandwidth system is used to record the backreflected light from the main laser amplifiers and elsewhere. The recording electronics are discussed in detail.

Project implementation : classification of organic soils and classification of marls - training of INDOT personnel.

Science.gov (United States)

2012-09-01

This is an implementation project for the research completed as part of the following projects: SPR3005 Classification of Organic Soils : and SPR3227 Classification of Marl Soils. The methods developed for the classification of both soi...

The Magnetic Recoil Spectrometer for time-resolved neutron measurements (MRSt) at the NIF

Science.gov (United States)

Parker, C. E.; Frenje, J. A.; Wink, C. W.; Gatu Johnson, M.; Lahmann, B.; Li, C. K.; Seguin, F. H.; Petrasso, R. D.; Hilsabeck, T. J.; Kilkenny, J. D.; Bionta, R.; Casey, D. T.; Khater, H. Y.; Forrest, C. J.; Glebov, V. Yu.; Sorce, C.; Hares, J. D.; Siegmund, O. H. W.

2017-10-01

The next-generation Magnetic Recoil Spectrometer, called MRSt, will provide time-resolved measurements of the DT-neutron spectrum. These measurements will provide critical information about the time evolution of the fuel assembly, hot-spot formation, and nuclear burn in Inertial Confinement Fusion (ICF) implosions at the National Ignition Facility (NIF). The neutron spectrum in the energy range 12-16 MeV will be measured with high accuracy ( 5%), unprecedented energy resolution ( 100 keV) and, for the first time ever, time resolution ( 20 ps). An overview of the physics motivation, conceptual design for meeting these performance requirements, and the status of the offline tests for critical components will be presented. This work was supported in part by the U.S. DOE, LLNL, and LLE.

Characterizing NIF hohlraum energy and particle transport using mid-Z spectroscopic tracer materials

Science.gov (United States)

Moody, J. D.; Barrios, M. A.; Widmann, K.; Suter, L. J.; Liedahl, D. A.; Schneider, M. B.; Thorn, D. B.; Farmer, W. A.; Landen, O. L.; Kauffman, R. L.; Jarrott, C.; Sherlock, M. W.; Chen, H.; Jones, O.; MacLaren, S. A.; Eder, D.; Strozzi, D. J.; Meezan, N. B.; Nikroo, A.; Kroll, J. J.; Johnson, S.; Jaquez, J.; Huang, H.

2017-10-01

Line emission from mid-Z dopants placed at several spatial locations is used to determine the electron temperature (Te) and plasma flow in NIF hohlraums. Laser drive ablates the dopant and launches it on a trajectory recorded with a framing camera. Analysis of temporally streaked spectroscopy provides an estimate of the time-resolved Te. The estimated temperature gradients show evidence for significantly restricted thermal conduction. Non-local thermal conductivity can account for part of this; additional effects due to magnetic fields, return-current instabilities, ion acoustic turbulence and other physics are considered. We describe our findings and discuss interpretations. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

Organization of visual cortical projections to the claustrum in the cat

International Nuclear Information System (INIS)

Jayaraman, A.; Updyke, B.V.

1979-01-01

The projection patterns from different visual areas of the parieto-occipital cortex to the claustrum were studied autoradiographically in cats. When [ 3 H] was injected into 17, 18, 19 or Clare-Bishop areas, the label was transported to an area restricted to the dorsal and caudal parts of the claustrum without any suggestion of retinotopic organization. Injection in each of these visual areas resulted in individual patterns of projection but with overlapping fields of termination, a pattern similar to corticocaudate projection. When injected into area 7, a region shown to have neurons involved in visuomotor mechanisms, the label was transported to the same area as that of the visual projection. These and other findings suggest that claustrum may be reciprocally and topographically connected with the cerebral cortex. (Auth.)

Diagnosing implosion performance at the National Ignition Facility (NIF) by means of neutron spectrometry

International Nuclear Information System (INIS)

Frenje, J.A.; Casey, D.T.; Johnson, M. Gatu; Bionta, R.; Bond, E.J.; Caggiano, J.A.; Cerjan, C.; Edwards, J.; Eckart, M.; Fittinghoff, D.N.; Friedrich, S.; Glenzer, S.; Haan, S.; Hatarik, R.; Hatchett, S.; Jones, O.S.; Glebov, V.Yu.; Knauer, J.P.; Grim, G.; Kilkenny, J.D.

2013-01-01

The neutron spectrum from a cryogenically layered deuterium–tritium (dt) implosion at the National Ignition Facility (NIF) provides essential information about the implosion performance. From the measured primary-neutron spectrum (13–15 MeV), yield (Y n ) and hot-spot ion temperature (T i ) are determined. From the scattered neutron yield (10–12 MeV) relative to Y n , the down-scatter ratio, and the fuel areal density (ρR) are determined. These implosion parameters have been diagnosed to an unprecedented accuracy with a suite of neutron-time-of-flight spectrometers and a magnetic recoil spectrometer implemented in various locations around the NIF target chamber. This provides good implosion coverage and excellent measurement complementarity required for reliable measurements of Y n , T i and ρR, in addition to ρR asymmetries. The data indicate that the implosion performance, characterized by the experimental ignition threshold factor, has improved almost two orders of magnitude since the first shot taken in September 2010. ρR values greater than 1 g cm −2 are readily achieved. Three-dimensional semi-analytical modelling and numerical simulations of the neutron-spectrometry data, as well as other data for the hot spot and main fuel, indicate that a maximum hot-spot pressure of ∼150 Gbar has been obtained, which is almost a factor of two from the conditions required for ignition according to simulations. Observed Y n are also 3–10 times lower than predicted. The conjecture is that the observed pressure and Y n deficits are partly explained by substantial low-mode ρR asymmetries, which may cause inefficient conversion of shell kinetic energy to hot-spot thermal energy at stagnation. (paper)

NeuroMorpho.Org implementation of digital neuroscience: dense coverage and integration with the NIF.

Science.gov (United States)

Halavi, Maryam; Polavaram, Sridevi; Donohue, Duncan E; Hamilton, Gail; Hoyt, Jeffrey; Smith, Kenneth P; Ascoli, Giorgio A

2008-09-01

Neuronal morphology affects network connectivity, plasticity, and information processing. Uncovering the design principles and functional consequences of dendritic and axonal shape necessitates quantitative analysis and computational modeling of detailed experimental data. Digital reconstructions provide the required neuromorphological descriptions in a parsimonious, comprehensive, and reliable numerical format. NeuroMorpho.Org is the largest web-accessible repository service for digitally reconstructed neurons and one of the integrated resources in the Neuroscience Information Framework (NIF). Here we describe the NeuroMorpho.Org approach as an exemplary experience in designing, creating, populating, and curating a neuroscience digital resource. The simple three-tier architecture of NeuroMorpho.Org (web client, web server, and relational database) encompasses all necessary elements to support a large-scale, integrate-able repository. The data content, while heterogeneous in scientific scope and experimental origin, is unified in format and presentation by an in house standardization protocol. The server application (MRALD) is secure, customizable, and developer-friendly. Centralized processing and expert annotation yields a comprehensive set of metadata that enriches and complements the raw data. The thoroughly tested interface design allows for optimal and effective data search and retrieval. Availability of data in both original and standardized formats ensures compatibility with existing resources and fosters further tool development. Other key functions enable extensive exploration and discovery, including 3D and interactive visualization of branching, frequently measured morphometrics, and reciprocal links to the original PubMed publications. The integration of NeuroMorpho.Org with version-1 of the NIF (NIFv1) provides the opportunity to access morphological data in the context of other relevant resources and diverse subdomains of neuroscience, opening

Hot spot mix in ICF implosions on the NIF

Science.gov (United States)

Ma, Tammy

2016-10-01

In the quest to achieve ignition through the inertial confinement fusion scheme, one of the critical challenges is to drive a symmetric implosion at high velocity without hydrodynamic instabilities becoming detrimental. These instabilities, primarily at the ablation front and the fuel-ablator interface, can cause mix of the higher-Z shell into the hot spot, resulting in increased radiation loss and thus reduced temperature and neutron yield. To quantify the level of mix, we developed a model that infers the level of hot spot contamination using the ratio of the enhanced x-ray production relative to the neutron yield. Applying this methodology to the full ensemble of indirect-drive National Ignition Facility (NIF) cryogenically layered DT implosions provides insight on the sensitivity of performance to the level of ablator-hot spot mix. In particular, the improvement seen with the High Foot design can be primarily attributed to a reduction in ablation-front instability mix that enabled the implosions to be pushed to higher velocity and performance. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344, Lawrence Livermore National Security, LLC.

First Laser-Plasma Interaction and Hohlraum Experiments on NIF

International Nuclear Information System (INIS)

Dewald, E L; Glenzer, S H; Landen, O L; Suter, L J; Jones, O S; Schein, J; Froula, D; Divol, L; Campbell, K; Schneider, M S; McDonald, J W; Niemann, C; Mackinnon, A J

2005-01-01

Recently the first hohlraum experiments have been performed at the National Ignition Facility (NIF) in support of indirect drive Inertial Confinement Fusion (ICF) designs. The effects of laser beam smoothing by spectral dispersion (SSD) and polarization smoothing (PS) on the beam propagation in long scale gas-filled pipes has been studied at plasma scales as found in indirect drive gas filled ignition hohlraum designs. The long scale gas-filled target experiments have shown propagation over 7 mm of dense plasma without filamentation and beam break up when using full laser smoothing. Vacuum hohlraums have been irradiated with laser powers up to 6 TW, 1-9 ns pulse lengths and energies up to 17 kJ to activate several diagnostics, to study the hohlraum radiation temperature scaling with the laser power and hohlraum size, and to make contact with hohlraum experiments performed at the NOVA and Omega laser facilities. Subsequently, novel long laser pulse hohlraum experiments have tested models of hohlraum plasma filling and long pulse hohlraum radiation production. The validity of the plasma filling assessment in analytical models and in LASNEX calculations has been proven for the first time. The comparison of these results with modeling will be discussed

THE THEORETIC-METHODOLOGICAL TOOLS OF EFFECTIVE ESTIMATION OF ORGANIZATION TECHNOLOGICAL SOLUTIONS OF CONSTRUCTION PROJECT

Directory of Open Access Journals (Sweden)

E. Yu. Antipenko

2010-03-01

Full Text Available In the article the structural analysis of efficiency indices elements of organization-and-technology solutions of construction project scheduling is executed for preparation of high-quality base of providing the planning processes and subsequent realization of the projects.

In-house coordination project for organ and tissue procurement: social responsibility and promising results.

Science.gov (United States)

Silva, Vanessa Silva E; Moura, Luciana Carvalho; Martins, Luciana Ribeiro; Santos, Roberta Cristina Cardoso Dos; Schirmer, Janine; Roza, Bartira de Aguiar

2016-01-01

to report the results of evaluation regarding changes in the number of potential donor referrals, actual donors, and conversion rates after the implementation of an in-house organ and tissue donation for transplantation coordination project. epidemiological study, both retrospective and transversal, was performed with organ donation data from the Secretariat of Health for the State and the in-house organ donation coordination project of a beneficent hospital. The data was compared using nonparametric statistical Mann-Whitney test, and the Student's t-test, considering a significance level of 5% (p <0.05). there were statistically significant differences (p < 0.05), before and after the implementation of the project on the number of potential donor notification/month (3.05 - 4.7 ), number of actual donor/month (0.78 to 1.60) and rate of conversion ( 24.7 to 34.8 %). The hospitals 1, 2, 7 and 8 had significant results in potential donor, actual donor or conversion rate. the presence of an in-house coordinator is promising and beneficial, the specialist is important to change the indicators of efficiency, which consequently reduces the waiting lists for organ transplants.

The Impact of Organization, Project and Governance Variables on Software Quality and Project Success

OpenAIRE

Abbas, Noura; Gravell, Andy; Wills, Gary

2010-01-01

In this paper we present a statistically tested evidence about how quality and success rate are correlated with variables reflecting the organization and aspects of its project’s governance, namely retrospectives and metrics. The results presented in this paper are based on the Agile Projects Governance Survey that collected 129 responses. This paper discuss the deep analysis of this survey, and the main findings suggest that when applying agile software development, the quality of software i...

Exploring nuclear reactions relevant to Stellar and Big-Bang Nucleosynthesis using High-Energy-Density plasmas at OMEGA and the NIF

Science.gov (United States)

Gatu Johnson, M.

2017-10-01

Thermonuclear reaction rates and nuclear processes have been explored traditionally by means of accelerator experiments, which are difficult to execute at conditions relevant to Stellar Nucleosynthesis (SN) and Big Bang Nucleosynthesis (BBN). High-Energy-Density (HED) plasmas closely mimic astrophysical environments and are an excellent complement to accelerator experiments in exploring SN and BBN-relevant nuclear reactions. To date, our work using HED plasmas at OMEGA and NIF has focused on the complementary 3He+3He, T+3He and T +T reactions. First studies of the T +T reaction indicated the significance of the 5He ground-state resonance in the T +T neutron spectrum. Subsequent T +T experiments showed that the strength of this resonance varies with center-of-mass (c-m) energy in the range of 16-50 keV, a variation that is not fundamentally understood. Studies of the 3He+3He and T+3He reactions have also been conducted at OMEGA at c-m energies of 165 keV and 80 keV, respectively, and the results revealed three things. First, a large cross section for the T+3He- γ branch can be ruled out as an explanation for the anomalously high abundance of 6Li in primordial material. Second, the results contrasted to theoretical modeling indicate that the mirror-symmetry assumption is not enough to capture the differences between T +T and 3He+3He reactions. Third, the elliptical spectrum assumed in the analysis of 3He+3He data obtained in accelerator experiments is incorrect. Preliminary data from recent experiments at the NIF exploring the 3He+3He reaction at c-m energies of 60 keV and 100 keV also indicate that the underlying physics changes with c-m energy. In this talk, we describe these findings and future directions for exploring light-ion reactions at OMEGA and the NIF. The work was supported in part by the US DOE, LLE, and LLNL.

Demonstration of Efficient Core Heating of Magnetized Fast Ignition in FIREX project

Science.gov (United States)

Johzaki, Tomoyuki

2017-10-01

Extensive theoretical and experimental research in the FIREX ``I project over the past decade revealed that the large angular divergence of the laser generated electron beam is one of the most critical problems inhibiting efficient core heating in electron-driven fast ignition. To solve this problem, beam guiding using externally applied kilo-tesla class magnetic field was proposed, and its feasibility has recently been numerically demonstrated. In 2016, integrated experiments at ILE Osaka University demonstrated core heating efficiencies reaching > 5 % and heated core temperatures of 1.7 keV. In these experiments, a kilo-tesla class magnetic field was applied to a cone-attached Cu(II) oleate spherical solid target by using a laser-driven capacitor-coil. The target was then imploded by G-XII laser and heated by the PW-class LFEX laser. The heating efficiency was evaluated by measuring the number of Cu-K- α photons emitted. The heated core temperature was estimated by the X-ray intensity ratio of Cu Li-like and He-like emission lines. To understand the detailed dynamics of the core heating process, we carried out integrated simulations using the FI3 code system. Effects of magnetic fields on the implosion and electron beam transport, detailed core heating dynamics, and the resultant heating efficiency and core temperature will be presented. I will also discuss the prospect for an ignition-scale design of magnetized fast ignition using a solid ball target. This work is partially supported by JSPA KAKENHI Grant Number JP16H02245, JP26400532, JP15K21767, JP26400532, JP16K05638 and is performed with the support and the auspices of the NIFS Collaboration Research program (NIFS12KUGK057, NIFS15KUGK087).

Processors for wavelet analysis and synthesis: NIFS and TI-C80 MVP

Science.gov (United States)

Brooks, Geoffrey W.

1996-03-01

Two processors are considered for image quadrature mirror filtering (QMF). The neuromorphic infrared focal-plane sensor (NIFS) is an existing prototype analog processor offering high speed spatio-temporal Gaussian filtering, which could be used for the QMF low- pass function, and difference of Gaussian filtering, which could be used for the QMF high- pass function. Although not designed specifically for wavelet analysis, the biologically- inspired system accomplishes the most computationally intensive part of QMF processing. The Texas Instruments (TI) TMS320C80 Multimedia Video Processor (MVP) is a 32-bit RISC master processor with four advanced digital signal processors (DSPs) on a single chip. Algorithm partitioning, memory management and other issues are considered for optimal performance. This paper presents these considerations with simulated results leading to processor implementation of high-speed QMF analysis and synthesis.

Implementation of ISO 10110 optics drawing standards for the National Ignition Facility

International Nuclear Information System (INIS)

Aikens, D. M.; English, R. E.; Wang, D. Y.

1999-01-01

The National Ignition Facility (NIF) project elected to implement ISO 10110 standard for the specifications of NIF optics drawings in 1996. More than 7,000 NIF large optics and 20,000 NIF small optics will be manufactured based on ISO 10110 indications. ISO 10110 standard meets many of the needs of the NIF optics specifications. It allows the optical engineer to quantify and clearly communicate the desired optical specifications. While no single drawing standard specifies all the requirements of high energy laser system, a combination of ISO 10110 standard with detailed notes make it possible to apply international drawing standards to the NIF laser system. This paper will briefly describe LLNL's interpretation and implementation of the ISO 10110 drawing standard, present some examples of NIF optics drawings, and discuss pros and cons of the indications from the perspective of this application. Emphasis will be given to the surface imperfection specifications, known as 5/, for the NIF optics

Implementation of ISO 10110 optics drawing standards for the National Ignition Facility

Science.gov (United States)

Wang, David Y.; English, R. Edward, Jr.; Aikens, David M.

1999-11-01

The National Ignition Facility (NIF) project elected to implement ISO 10110 standard for the specifications of NIF optics drawings in 1996. More than 7,000 NIF large optics and 20,000 NIF small optics will be manufactured based on ISO 10110 indications. ISO 10110 standard meets many of the needs of the NIF optics specifications. It allows the optical engineer to quantify and clearly communicate the desired optical specifications. While no single drawing standard specifies all the requirements of high energy laser system, a combination of ISO 10110 standard with detailed notes make it possible to apply international drawing standards to the NIF laser system. This paper will briefly describe LLNL's interpretation and implementation of the ISO 10110 drawing standard, present some examples of NIF optics drawings, and discuss pros and cons of the indications from the perspective of this application. Emphasis will be given to the surface imperfection specifications, known as 5/, for the NIF optics.

Matching of Resources and the Design of Organizations for Project Management

NARCIS (Netherlands)

Cunningham, S.

2012-01-01

This paper addresses a problem common to many high-technology firms. How can firms select and resource appropriate projects while balancing the needs of management as well as technologists? We argue that such problems result in social dilemmas for organizations, requiring practical institutional

Modeling Laser-Plasma Interaction over a Suite of NIF Experiments

Science.gov (United States)

Strozzi, D. J.; Berger, R. L.; Jones, O. S.; Chapman, T.; Woods, D. T.; MacLaren, S. A.; Michel, P.; Divol, L.

2017-10-01

We systematically study laser-plasma interaction (LPI) on NIF indirect-drive experiments, namely backscatter and cross-beam energy transfer. LLNL's best practice radiation-hydrodynamic simulation methodology in the Lasnex simulation code is employed without ad-hoc tuning to match experimental data. This entails converged numerical resolution, an improved DCA model for coronal (ne 1 keV) gold opacity, electron heat flux strongly limited to 0.03neTe3 / 2 me- 1 / 2 , and the inline CBET model. The rad-hydro plasma conditions are used for LPI analysis, namely linear instability gains, and the paraxial-envelope code pF3D. Simulated scattered-light spectra are also compared to measurements. We initially focus on shots with low backscatter, so its self-consistent treatment should not be important. These shots have low hohlraum fill density and short laser pulses, and the only significant backscatter is outer-beams Brillouin. Our long-term goals are to understand reflectivity trends to guide target design and develop LPI mitigation strategies. Work performed under auspices of US DoE by LLNL under Contract DE-AC52-07NA27344.

Advanced Low Temperature Geothermal Power Cycles (The ENTIV Organic Project) Final Report

Energy Technology Data Exchange (ETDEWEB)

Mugerwa, Michael [Technip USA, Inc., Claremont, CA (United States)

2015-11-18

Feasibility study of advanced low temperature thermal power cycles for the Entiv Organic Project. Study evaluates amonia-water mixed working fluid energy conversion processes developed and licensed under Kalex in comparison with Kalina cycles. Both cycles are developed using low temperature thermal resource from the Lower Klamath Lake Geothermal Area. An economic feasibility evaluation was conducted for a pilot plant which was deemed unfeasible by the Project Sponsor (Entiv).

A concept to collect neutron and x-ray images on the same line of sight at NIF

Energy Technology Data Exchange (ETDEWEB)

Merrill, F. E., E-mail: fmerrill@lanl.gov; Danly, C. R.; Grim, G. P.; Volegov, P. L.; Wilde, C. H. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Izumi, N.; Jedlovec, D.; Fittinghoff, D. N.; Pak, A.; Park, H.-S. [Livermore National Laboratory, Livermore, California 94551 (United States)

2014-11-15

Neutron and x-ray images are collected at the National Ignition Facility (NIF) to measure the size and shape of inertial confinement fusion implosions. The x-ray images provide a measure of the size and shape of the hot region of the deuterium-tritium fuel while the neutron images provide a measure of the size and shape of the burning plasma. Although these two types of images are collected simultaneously, they are not collected along the same line of sight (LOS). One 14 MeV neutron image is collected on the NIF equator, and two x-ray images are collected along the polar axis and nearly perpendicular to the neutron imaging line of sight on the equator. Both measurements use pinhole apertures to form the images, but existing x-ray imaging provides time-resolved measurements while the neutron images are time-integrated. Detailed comparisons of the x-ray and neutron images can provide information on the fuel assembly, but these studies have been limited because the implosions are not azimuthally symmetric and the images are collected along different LOS. We have developed a conceptual design of a time-integrated x-ray imaging system that could be added to the existing neutron imaging LOS. This new system would allow these detailed studies, providing important information on the fuel assembly of future implosions. Here we present this conceptual design and the expected performance characteristics.

A concept to collect neutron and x-ray images on the same line of sight at NIF.

Science.gov (United States)

Merrill, F E; Danly, C R; Izumi, N; Jedlovec, D; Fittinghoff, D N; Grim, G P; Pak, A; Park, H-S; Volegov, P L; Wilde, C H

2014-11-01

Neutron and x-ray images are collected at the National Ignition Facility (NIF) to measure the size and shape of inertial confinement fusion implosions. The x-ray images provide a measure of the size and shape of the hot region of the deuterium-tritium fuel while the neutron images provide a measure of the size and shape of the burning plasma. Although these two types of images are collected simultaneously, they are not collected along the same line of sight (LOS). One 14 MeV neutron image is collected on the NIF equator, and two x-ray images are collected along the polar axis and nearly perpendicular to the neutron imaging line of sight on the equator. Both measurements use pinhole apertures to form the images, but existing x-ray imaging provides time-resolved measurements while the neutron images are time-integrated. Detailed comparisons of the x-ray and neutron images can provide information on the fuel assembly, but these studies have been limited because the implosions are not azimuthally symmetric and the images are collected along different LOS. We have developed a conceptual design of a time-integrated x-ray imaging system that could be added to the existing neutron imaging LOS. This new system would allow these detailed studies, providing important information on the fuel assembly of future implosions. Here we present this conceptual design and the expected performance characteristics.

Model and Algorithm for Substantiating Solutions for Organization of High-Rise Construction Project

Directory of Open Access Journals (Sweden)

Anisimov Vladimir

2018-01-01

Full Text Available In the paper the models and the algorithm for the optimal plan formation for the organization of the material and logistical processes of the high-rise construction project and their financial support are developed. The model is based on the representation of the optimization procedure in the form of a non-linear problem of discrete programming, which consists in minimizing the execution time of a set of interrelated works by a limited number of partially interchangeable performers while limiting the total cost of performing the work. The proposed model and algorithm are the basis for creating specific organization management methodologies for the high-rise construction project.

Model and Algorithm for Substantiating Solutions for Organization of High-Rise Construction Project

Science.gov (United States)

Anisimov, Vladimir; Anisimov, Evgeniy; Chernysh, Anatoliy

2018-03-01

In the paper the models and the algorithm for the optimal plan formation for the organization of the material and logistical processes of the high-rise construction project and their financial support are developed. The model is based on the representation of the optimization procedure in the form of a non-linear problem of discrete programming, which consists in minimizing the execution time of a set of interrelated works by a limited number of partially interchangeable performers while limiting the total cost of performing the work. The proposed model and algorithm are the basis for creating specific organization management methodologies for the high-rise construction project.

The national ignition facility: path to ignition in the laboratory

International Nuclear Information System (INIS)

Moses, E.I.; Bonanno, R.E.; Haynam, C.A.; Kauffman, R.L.; MacGowan, B.J.; Patterson Jr, R.W.; Sawicki, R.H.; Van Wonterghem, B.M.

2007-01-01

The National Ignition Facility (NIF) is a 192-beam laser facility presently under construction at Lawrence Livermore National Laboratory. When completed, NIF will be a 1.8-MJ, 500-TW ultraviolet laser system. Its missions are to obtain fusion ignition of deuterium-tritium plasmas in ICF (Inertial Confinement Fusion) targets and to perform high energy density experiments in support of the U.S. nuclear weapons stockpile. The NIF facility will consist of 2 laser bays, 4 capacitor areas, 2 laser switchyards, the target area and the building core. The laser is configured in 4 clusters of 48 beams, 2 in each laser bay. Four of the NIF beams have been already commissioned to demonstrate laser performance and to commission the target area including target and beam alignment and laser timing. During this time, NIF has demonstrated on a single-beam basis that it will meet its performance goals and has demonstrated its precision and flexibility for pulse shaping, pointing, timing and beam conditioning. It also performed 4 important experiments for ICF and High Energy Density Science. Presently, the project is installing production hardware to complete the project in 2009 with the goal to begin ignition experiments in 2010. An integrated plan has been developed including the NIF operations, user equipment such as diagnostics and cryogenic target capability, and experiments and calculations to meet this goal. This talk will provide NIF status, the plan to complete NIF, and the path to ignition. (authors)

Leadership in project-based organizations: Dealing with complex and paradoxical demands

NARCIS (Netherlands)

Havermans, L.A.

2014-01-01

Despite the overwhelming amount of attention given by scientists and practitioners to leadership, most theories of leadership have been developed to explain the role of leadership in traditional line organizations, not to explain how leaders deal with complex and paradoxical demands in project-based

Organization of project management

International Nuclear Information System (INIS)

Schmidt, R.

1975-01-01

When speaking about interfaces within a project and their management, one has to understand and define what an interface is. In general, each component facing another one and each person working on a project with another person represents an interface. Therefore a project will consist practically in its entirety of interfaces with components and people sandwiched between them. This paper is limited to the most important interfaces with a focus on the problems occuring at them and their resolution. (orig.) [de

Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer

NARCIS (Netherlands)

Coelho, M.R.R.; Vos, de M.; Carneiro, N.P.; Marriel, I.E.; Paiva, E.; Seldin, L.

2008-01-01

The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha(-1)). The nifH gene was amplified directly from DNA extracted from the rhizospheres,

ORGANIZATION OF INDEPENDENT WORK STUDENTS OF MASTER OF TELECOMMUNICATION TRAINING PROJECT

Directory of Open Access Journals (Sweden)

L. Perminova

2013-03-01

Full Text Available The article explains the need to implement active learning of students in the practice of the organization їh homework. In particular, the author proved the rationality of the use of telecommunications projects and use the web in quest of independent study students graduate.

A Neural Code That Is Isometric to Vocal Output and Correlates with Its Sensory Consequences.

Directory of Open Access Journals (Sweden)

Alexei L Vyssotski

2016-10-01

Full Text Available What cortical inputs are provided to motor control areas while they drive complex learned behaviors? We study this question in the nucleus interface of the nidopallium (NIf, which is required for normal birdsong production and provides the main source of auditory input to HVC, the driver of adult song. In juvenile and adult zebra finches, we find that spikes in NIf projection neurons precede vocalizations by several tens of milliseconds and are insensitive to distortions of auditory feedback. We identify a local isometry between NIf output and vocalizations: quasi-identical notes produced in different syllables are preceded by highly similar NIf spike patterns. NIf multiunit firing during song precedes responses in auditory cortical neurons by about 50 ms, revealing delayed congruence between NIf spiking and a neural representation of auditory feedback. Our findings suggest that NIf codes for imminent acoustic events within vocal performance.

Can Maine metropolitan planning organizations (MPOs) finance transportation projects through bond financing?

Science.gov (United States)

2008-12-01

In January 2008 the Maine metropolitan planning organizations (MPOs) requested the Transportation : Research Division of the Maine Department of Transportation to conduct research to determine if it is : possible for MPOs to finance projects through ...

Expression of the N2 fixation gene operon of Paenibacillus sp. WLY78 under the control of the T7 promoter in Escherichia coli BL21.

Science.gov (United States)

Zhang, Lihong; Liu, Xiaomeng; Li, Xinxin; Chen, Sanfeng

2015-10-01

To investigate the transcription and translation and nitrogenase activity of the nine N2-fixing-gene (nif) operon (nifBHDKENXhesAnifX) of Paenibacillus sp. WLY78 under the control of the T7 promoter in Escherichia coli BL21 under different conditions. The Paenibacillus nif operon under the control of the T7 promoter is significantly transcribed and effectively translated in E. coli BL21 when grown in medium containing organic N compounds (yeast extract and Tryptone) or NH4+ by using RT-PCR and Western blot analysis. Transcription and translation of foreign nif genes in E. coli are not inhibited by environmental organic or inorganic N compounds or O2. However, contrary to transcription and translation, nitrogenase activity is 4% lower in the recombinant E. coli 78-32 compared to the native Paenibacillus sp. WLY78. The Paenibacillus nif operon under the control of T7 promoter enables E. coli BL21 to synthesize active nitrogenase. This study shows how the nif gene operon can be transferred to non-N2-fixing bacteria or to eukaryotic organelles.