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Sample records for nci-h838 cells accompanied

  1. Metastatic Basal Cell Carcinoma Accompanying Gorlin Syndrome

    Directory of Open Access Journals (Sweden)

    Yeliz Bilir

    2014-01-01

    Full Text Available Gorlin-Goltz syndrome or basal cell nevus syndrome is an autosomal dominant syndrome characterized by skeletal anomalies, numerous cysts observed in the jaw, and multiple basal cell carcinoma of the skin, which may be accompanied by falx cerebri calcification. Basal cell carcinoma is the most commonly skin tumor with slow clinical course and low metastatic potential. Its concomitance with Gorlin syndrome, resulting from a mutation in a tumor suppressor gene, may substantially change morbidity and mortality. A 66-year-old male patient with a history of recurrent basal cell carcinoma was presented with exophthalmus in the left eye and the lesions localized in the left lateral orbita and left zygomatic area. His physical examination revealed hearing loss, gapped teeth, highly arched palate, and frontal prominence. Left orbital mass, cystic masses at frontal and ethmoidal sinuses, and multiple pulmonary nodules were detected at CT scans. Basal cell carcinoma was diagnosed from biopsy of ethmoid sinus. Based on the clinical and typical radiological characteristics (falx cerebri calcification, bifid costa, and odontogenic cysts, the patient was diagnosed with metastatic skin basal cell carcinoma accompanied by Gorlin syndrome. Our case is a basal cell carcinoma with aggressive course accompanying a rarely seen syndrome.

  2. Metastatic Basal cell carcinoma accompanying gorlin syndrome.

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    Bilir, Yeliz; Gokce, Erkan; Ozturk, Banu; Deresoy, Faik Alev; Yuksekkaya, Ruken; Yaman, Emel

    2014-01-01

    Gorlin-Goltz syndrome or basal cell nevus syndrome is an autosomal dominant syndrome characterized by skeletal anomalies, numerous cysts observed in the jaw, and multiple basal cell carcinoma of the skin, which may be accompanied by falx cerebri calcification. Basal cell carcinoma is the most commonly skin tumor with slow clinical course and low metastatic potential. Its concomitance with Gorlin syndrome, resulting from a mutation in a tumor suppressor gene, may substantially change morbidity and mortality. A 66-year-old male patient with a history of recurrent basal cell carcinoma was presented with exophthalmus in the left eye and the lesions localized in the left lateral orbita and left zygomatic area. His physical examination revealed hearing loss, gapped teeth, highly arched palate, and frontal prominence. Left orbital mass, cystic masses at frontal and ethmoidal sinuses, and multiple pulmonary nodules were detected at CT scans. Basal cell carcinoma was diagnosed from biopsy of ethmoid sinus. Based on the clinical and typical radiological characteristics (falx cerebri calcification, bifid costa, and odontogenic cysts), the patient was diagnosed with metastatic skin basal cell carcinoma accompanied by Gorlin syndrome. Our case is a basal cell carcinoma with aggressive course accompanying a rarely seen syndrome.

  3. Massively augmented hippocampal dentate granule cell activation accompanies epilepsy development

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    Dengler, Christopher G.; Yue, Cuiyong; Takano, Hajime; Coulter, Douglas A.

    2017-01-01

    In a mouse model of temporal lobe epilepsy, multicellular calcium imaging revealed that disease emergence was accompanied by massive amplification in the normally sparse, afferent stimulation-induced activation of hippocampal dentate granule cells. Patch recordings demonstrated reductions in local inhibitory function within the dentate gyrus at time points where sparse activation was compromised. Mimicking changes in inhibitory synaptic function and transmembrane chloride regulation was sufficient to elicit the dentate gyrus circuit collapse evident during epilepsy development. Pharmacological blockade of outward chloride transport had no effect during epilepsy development, and significantly increased granule cell activation in both control and chronically epileptic animals. This apparent occlusion effect implicates reduction in chloride extrusion as a mechanism contributing to granule cell hyperactivation specifically during early epilepsy development. Glutamine plays a significant role in local synthesis of GABA in synapses. In epileptic mice, sparse granule cell activation could be restored by glutamine application, implicating compromised GABA synthesis. Glutamine had no effect on granule cell activation earlier, during epilepsy development. We conclude that compromised feedforward inhibition within the local circuit generates the massive dentate gyrus circuit hyperactivation evident in animals during and following epilepsy development. However, the mechanisms underlying this disinhibition diverge significantly as epilepsy progresses. PMID:28218241

  4. Apoptosis during embryonic tissue remodeling is accompanied by cell senescence

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    Lorda-Diez, Carlos I.; Garcia-Riart, Beatriz; Montero, Juan A.; Rodriguez-León, Joaquín; Garcia-Porrero, Juan A; Hurle, Juan M.

    2015-01-01

    This study re-examined the dying process in the interdigital tissue during the formation of free digits in the developing limbs. We demonstrated that the interdigital dying process was associated with cell senescence, as deduced by induction of β-gal activity, mitotic arrest, and transcriptional up-regulation of p21 together with many components of the senescence-associated secretory phenotype. We also found overlapping domains of expression of members of the Btg/Tob gene family of antiproliferative factors in the regressing interdigits. Notably, Btg2 was up-regulated during interdigit remodeling in species with free digits but not in the webbed foot of the duck. We also demonstrate that oxidative stress promoted the expression of Btg2, and that FGF2 and IGF1 which are survival signals for embryonic limb mesenchyme inhibited Btg2 expression. Btg2 overexpression in vivo and in vitro induced all the observed changes during interdigit regression, including oxidative stress, arrest of cell cycle progression, transcriptional regulation of senescence markers, and caspase-mediated apoptosis. Consistent with the central role of p21 on cell senescence, the transcriptional effects induced by overexpression of Btg2 are attenuated by silencing p21. Our findings indicate that cell senescence and apoptosis are complementary processes in the regression of embryonic tissues and share common regulatory signals. PMID:26568417

  5. Apoptosis during embryonic tissue remodeling is accompanied by cell senescence

    OpenAIRE

    2015-01-01

    This study re-examined the dying process in the interdigital tissue during the formation of free digits in the developing limbs. We demonstrated that the interdigital dying process was associated with cell senescence, as deduced by induction of β-gal activity, mitotic arrest, and transcriptional up-regulation of p21 together with many components of the senescence-associated secretory phenotype. We also found overlapping domains of expression of members of the Btg/Tob gene family of antiprolif...

  6. Anti-metastatic Potential of Amide-linked Local Anesthetics: Inhibition of Lung Adenocarcinoma Cell Migration and Inflammatory Src Signaling Independent of Sodium Channel Blockade

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    Piegeler, Tobias; Votta-Velis, E. Gina; Liu, Guoquan; Place, Aaron T.; Schwartz, David E.; Beck-Schimmer, Beatrice; Minshall, Richard D.; Borgeat, Alain

    2012-01-01

    Background Retrospective analysis of patients undergoing cancer surgery suggests the use of regional anesthesia may reduce cancer recurrence and improve survival. Amide-linked local anesthetics have anti-inflammatory properties, although the mechanism of action in this regard is unclear. As inflammatory processes involving Src tyrosine protein kinase and intercellular adhesion molecule-1 are important in tumor growth and metastasis, we hypothesized that amide-linked local anesthetics may inhibit inflammatory Src-signaling involved in migration of adenocarcinoma cells. Methods NCI-H838 lung cancer cells were incubated with Tumor Necrosis Factor-α in absence/presence of ropivacaine, lidocaine, or chloroprocaine (1nM-100μM). Cell migration and total cell lysate Src-activation and Intercellular Adhesion Molecule-1 phosphorylation were assessed. The role of voltage-gated sodium-channels in the mechanism of local anesthetic effects was also evaluated. Results Ropivacaine treatment (100μM) of H838 cells for 20 minutes decreased basal Src activity by 62% (p=0.003), and both ropivacaine and lidocaine co-administered with Tumor Necrosis Factor-α statistically significantly decreased Src-activation and Intercellular Adhesion Molecule-1 phosphorylation, whereas chloroprocaine had no such effect. Migration of these cells at 4 hours was inhibited by 26% (p=0.005) in presence of 1μM ropivacaine and 21% by 1μM lidocaine (p=0.004). These effects of ropivacaine and lidocaine were independent of voltage-gated sodium-channel inhibition. Conclusions This study indicates that amide-, but not ester-linked local anesthetics may provide beneficial anti-metastatic effects. The observed inhibition of NCI-H838 cell migration by lidocaine and ropivacaine was associated with the inhibition of Tumor Necrosis Factor-α-induced Src-activation and Intercellular Adhesion Molecule-1 phosphorylation, providing the first evidence of a molecular mechanism which appears to be independent of their

  7. Autophagy Accompanied with Bisdemethoxycurcumin-induced Apoptosis in Non-small Cell Lung Cancer Cells

    Institute of Scientific and Technical Information of China (English)

    XU Jin Hong; YANG He Ping; ZHOU Xiang Dong; WANG Hai Jing; GONG Liang; TANG Chun Lan

    2015-01-01

    Objective To investigate the effects of bisdemethoxycurcumin (BDMC) on non-small cell lung cancer (NSCLC) cell line, A549, and the highly metastatic lung cancer 95D cells. Methods CCK-8 assay was used to assess the effect of BDMC on cytotoxicity. Flow cytometry was used to evaluate apoptosis. Western blot analysis, electron microscopy, and quantification of GFP-LC3 punctuates were used to test the effect of BDMC on autophagy and apoptosis of lung cancer cells. Results BDMC inhibited the viability of NSCLC cells, but had no cytotoxic effects on lung small airway epithelial cells (SAECs). The apoptotic cell death induced by BDMC was accompanied with the induction of autophagy in NSCLC cells. Blockage of autophagy by the autophagy inhibitor 3-methyladenine (3-MA) repressed the growth inhibitory effects and induction of apoptosis by BDMC. In addition, BDMC treatment significantly decreased smoothened (SMO) and the transcription factor glioma-associated oncogene 1 (Gli1) expression. Furthermore, depletion of Gli1 by siRNA and cyclopamine (a specific SMO inhibitor) induced autophagy. Conclusion Aberrant activation of Hedgehog (Hh) signaling has been implicated in several human cancers, including lung cancers. The present findings provide direct evidence that BDMC-induced autophagy plays a pro-death role in NSCLC, in part, by inhibiting Hedgehog signaling.

  8. Whole-cell chloride currents in rat astrocytes accompany changes in cell morphology.

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    Lascola, C D; Kraig, R P

    1996-04-15

    Astrocytes can change shape dramatically in response to increased physiological and pathological demands, yet the functional consequences of morphological change are unknown. We report the expression of Cl- currents after manipulations that alter astrocyte morphology. Whole-cell Cl- currents were elicited after (1) rounding up cells by brief exposure to trypsin; (2) converting cells from a flat polygonal to a process-bearing (stellate) morphology by exposure to serum-free Ringer's solution; and (3) swelling cells by exposure to hypo-osmotic solution. Zero-current potentials approximated the Nernst for Cl-, and rectification usually followed that predicted by the constant-field equation. We observed heterogeneity in the activation and inactivation kinetics, as well as in the relative degree of outward versus inward rectification. Cl- conductances were inhibited by 4, 4-diisothiocyanostilbene-2,2'-disulfonic acid (200 microM) and by Zn2+ (1 mM). Whole-cell Cl- currents were not expressed in cells without structural change. We investigated whether changes in cytoskeletal actin accompanying changes in astrocytic morphology play a role in the induction of shape-dependent Cl- currents. Cytochalasins, which disrupt actin polymers by enhancing actin-ATP hydrolysis, elicited whole-cell Cl- conductances in flat, polygonal astrocytes. In stellate cells, elevated intracellular Ca2+ (2 microM), which can depolymerize actin, enhanced Cl- currents, and high intracellular ATP (5 mM), required for repolymerization, reduced Cl- currents. Modulation of Cl- current by Ca2+ and ATP was blocked by concurrent whole-cell dialysis with phalloidin and DNase, respectively. Phalloidin stabilizes actin polymers and DNase inhibits actin polymerization. Dialysis with phalloidin also prevented hypo-osmotically activated Cl- currents. These results demonstrate how the expression of astrocyte Cl- currents can be dependent on cell morphology, the structure of actin, Ca2+ homeostasis, and metabolism.

  9. Primary CD56+ NK/T-cell lymphoma of the rectum accompanied with refractory ulcerative colitis.

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    Kakimoto, Kazuki; Inoue, Takuya; Nishikawa, Takashi; Ishida, Kumi; Kawakami, Ken; Kuramoto, Takanori; Abe, Yosuke; Morita, Eijiro; Murano, Naoko; Toshina, Ken; Murano, Mitsuyuki; Umegaki, Eiji; Egashira, Yutaro; Okuda, Junji; Tanigawa, Nobuhiko; Hirata, Ichiro; Katsu, Ken-Ichi; Higuchi, Kazuhide

    2008-01-01

    A case of primary NK/T-cell lymphoma of the rectum accompanied with ulcerative colitis (UC) in a 73-year-old man is reported. He had a 6-year history of repeated admission to our hospital for UC. Total colonoscopy performed 4 months after resolution of refractory UC complicated by cytomegalovirus colitis showed a markedly submucosal tumor in the rectum, which was histologically diagnosed as malignant lymphoma. The findings of computed tomography of the chest and abdomen, gallium scintigraphy, abdominal ultrasonography, and upper gastrointestinal endoscopy showed no abnormal lesions. Therefore, based on a diagnosis of localized rectal lymphoma with UC, proctocolectomy was performed. The resected specimen showed three submucosal tumors in the rectum with local nodal involvement. Histologically, the tumors were characterized by diffusely infiltrating sheets of large atypical lymphoid cells, which were negative for CD4, CD8, and CD20 but were positive for CD56, CD3, and granzyme B. The presence of Epstein-Barr virus (EBV) infection in neoplastic cells was shown by in situ hybridization for EBV-encoded early small RNA1 (EBER-1). Based on these findings, the patient was diagnosed with primary CD56+ NK/T-cell lymphoma of the rectum (stage IIE). This is the first case report of primary rectal NK/T-cell lymphoma accompanied with UC.

  10. Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells.

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    Znidar, Katarina; Bosnjak, Masa; Cemazar, Maja; Heller, Loree C

    2016-06-07

    In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of several proinflammatory cytokines. These observations implicate the binding and activation of intracellular DNA-specific pattern recognition receptors or DNA sensors in response to DNA electrotransfer. In tumors, IFNβ mRNA and protein levels significantly increased. The mRNAs of several DNA sensors were detected, and DAI, DDX60, and p204 tended to be upregulated. These effects were accompanied with reduced tumor growth and increased tumor necrosis. In B16.F10 cells in culture, IFNβ mRNA and protein levels were significantly upregulated. The mRNAs for several DNA sensors were present in these cells; DNA-dependent activator of interferon regulatory factor (DAI), DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (DDX60), and p204 were significantly upregulated while DDX60 protein levels were coordinately upregulated. Upregulation of DNA sensors in tumors could be masked by the lower transfection efficiency compared to in vitro or to dilution by other tumor cell types. Mirroring the observation of tumor necrosis, cells underwent a significant DNA concentration-dependent decrease in proliferation and survival. Taken together, these results indicate that DNA electrotransfer may cause the upregulation of several intracellular DNA sensors in B16.F10 cells, inducing effects in vitro and potentially in vivo.

  11. Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells

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    Katarina Znidar

    2016-01-01

    Full Text Available In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of several proinflammatory cytokines. These observations implicate the binding and activation of intracellular DNA-specific pattern recognition receptors or DNA sensors in response to DNA electrotransfer. In tumors, IFNβ mRNA and protein levels significantly increased. The mRNAs of several DNA sensors were detected, and DAI, DDX60, and p204 tended to be upregulated. These effects were accompanied with reduced tumor growth and increased tumor necrosis. In B16.F10 cells in culture, IFNβ mRNA and protein levels were significantly upregulated. The mRNAs for several DNA sensors were present in these cells; DNA-dependent activator of interferon regulatory factor (DAI, DEAD (Asp-Glu-Ala-Asp box polypeptide 60 (DDX60, and p204 were significantly upregulated while DDX60 protein levels were coordinately upregulated. Upregulation of DNA sensors in tumors could be masked by the lower transfection efficiency compared to in vitro or to dilution by other tumor cell types. Mirroring the observation of tumor necrosis, cells underwent a significant DNA concentration-dependent decrease in proliferation and survival. Taken together, these results indicate that DNA electrotransfer may cause the upregulation of several intracellular DNA sensors in B16.F10 cells, inducing effects in vitro and potentially in vivo.

  12. Whole-Cell Chloride Currents in Rat Astrocytes Accompany Changes in Cell Morphology

    OpenAIRE

    Lascola, Christopher D.; Kraig, Richard P.

    1996-01-01

    Astrocytes can change shape dramatically in response to increased physiological and pathological demands, yet the functional consequences of morphological change are unknown. We report the expression of Cl− currents after manipulations that alter astrocyte morphology. Whole-cell Cl− currents were elicited after (1) rounding up cells by brief exposure to trypsin; (2) converting cells from a flat polygonal to a process-bearing (stellate) morphology by exposure to serum-free Ringer’s solution; a...

  13. Cell differentiation along multiple pathways accompanied by changes in histone acetylation status.

    Science.gov (United States)

    Legartová, Soňa; Kozubek, Stanislav; Franek, Michal; Zdráhal, Zbyněk; Lochmanová, Gabriela; Martinet, Nadine; Bártová, Eva

    2014-04-01

    Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events, including differentiation. In this study, we analyzed acetylated forms of histones H2A, H2B, and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-, di-, and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes, mono-acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs, we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-, di-, and tri-acetylation of H4 were reduced, manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation, whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.

  14. Structural alteration of cell wall pectins accompanies pea development in response to cold.

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    Baldwin, Laëtitia; Domon, Jean-Marc; Klimek, John F; Fournet, Françoise; Sellier, Hélène; Gillet, Françoise; Pelloux, Jérôme; Lejeune-Hénaut, Isabelle; Carpita, Nicholas C; Rayon, Catherine

    2014-08-01

    Pea (Pisum sativum) cell wall metabolism in response to chilling was investigated in a frost-sensitive genotype 'Terese' and a frost-tolerant genotype 'Champagne'. Cell walls isolated from stipules of cold acclimated and non-acclimated plants showed that cold temperatures induce changes in polymers containing xylose, arabinose, galactose and galacturonic acid residues. In the tolerant cultivar Champagne, acclimation is accompanied by increases in homogalacturonan, xylogalacturonan and highly branched Rhamnogalacturonan I with branched and unbranched (1→5)-α-arabinans and (1→4)-β-galactans. In contrast, the sensitive cultivar Terese accumulates substantial amounts of (1→4)-β-xylans and glucuronoxylan, but not the pectins. Greater JIM7 labeling was observed in Champagne compared to Terese, indicating that cold acclimation also induces an increase in the degree of methylesterification of pectins. Significant decrease in polygalacturonase activities in both genotypes were observed at the end of cold acclimation. These data indicate a role for esterified pectins in cold tolerance. The possible functions for pectins and their associated arabinans and galactans in cold acclimation are discussed.

  15. Cancer cell-selective promoter recognition accompanies antitumor effect by glucocorticoid receptor-targeted gold nanoparticle

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    Sau, Samaresh; Agarwalla, Pritha; Mukherjee, Sudip; Bag, Indira; Sreedhar, Bojja; Pal-Bhadra, Manika; Patra, Chitta Ranjan; Banerjee, Rajkumar

    2014-05-01

    Nanoparticles, such as gold nanoparticles (GNP), upon convenient modifications perform multi tasks catering to many biomedical applications. However, GNP or any other type of nanoparticles is yet to achieve the feat of intracellular regulation of endogenous genes of choice such as through manipulation of a gene-promoter in a chromosome. As for gene modulation and delivery, GNP (or other nanoparticles) showed only limited gene therapy potential, which relied on the delivery of `exogenous' genes invoking gene knockdown or replacement. Practically, there are no instances for the nanoparticle-mediated promoter regulation of `endogenous' genes, more so, as a cancer selective phenomenon. In this regard, we report the development of a simple, easily modifiable GNP-formulation, which promoted/up-regulated the expression of a specific category of `endogenous' genes, the glucocorticoid responsive genes. This genetic up-regulation was induced in only cancer cells by modified GNP-mediated transcriptional activation of its cytoplasmic receptor, glucocorticoid receptor (GR). Normal cells and their GR remained primarily unperturbed by this GNP-formulation. The most potent gene up-regulating GNP-formulation down-regulated a cancer-specific proliferative signal, phospho-Akt in cancer cells, which accompanied retardation of tumor growth in the murine melanoma model. We show that GR-targeted GNPs may find potential use in the targeting and modulation of genetic information in cancer towards developing novel anticancer therapeutics.Nanoparticles, such as gold nanoparticles (GNP), upon convenient modifications perform multi tasks catering to many biomedical applications. However, GNP or any other type of nanoparticles is yet to achieve the feat of intracellular regulation of endogenous genes of choice such as through manipulation of a gene-promoter in a chromosome. As for gene modulation and delivery, GNP (or other nanoparticles) showed only limited gene therapy potential, which relied

  16. Psychosocial Accompaniment

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    Mary Watkins

    2015-08-01

    Full Text Available This essay advocates for a paradigm shift in psychology toward the activity and ethics of accompaniment. Accompaniment requires a reorientation of the subjectivity, interpersonal practices, and critical understanding of the accompanier so that (she can stand alongside others who desire listening, witnessing, advocacy, space to develop critical inquiry and research, and joint imagination and action to address desired and needed changes. The idea of “accompaniment” emerged in liberation theology in Latin America, and migrated into liberatory forms of psychology as “psychosocial accompaniment.” This essay explores accompaniment and its ethics from a phenomenological perspective, highlighting differences from mainstream stances in psychology. Attention is also given to the effects of accompaniment on the accompanier. Efforts to decolonize psychology require careful attention to the psychic decolonization of its practitioners and to the cultivation of decolonizing interpersonal practices that provide a relational and ethical foundation for joint research, restorative healing, and transformative action. Such practices endeavor through dialogue to build mutual respect and understanding, promote effective solidarity, and contribute to the empowerment of those marginalized. The decolonization of psychology should enable practitioners to be more effective in working for increased social, economic, and environmental justice; peace building and reconciliation; and local and global ecological sustainability.

  17. Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

    Science.gov (United States)

    Pokrzywinski, Kaytee L.; Tilney, Charles L.; Warner, Mark E.; Coyne, Kathryn J.

    2017-03-01

    Bacteria may play a role in regulating harmful algal blooms, but little is known about the biochemical and physiological changes associated with cell death induced by algicidal bacteria. Previous work characterized an algicidal exudate (IRI-160AA) produced by Shewanella sp. IRI-160 that is effective against dinoflagellates, while having little to no effect on other phytoplankton species in laboratory culture experiments. The objective of this study was to evaluate biochemical changes associated with cell death and impacts on the cell cycle in three dinoflagellate species (Prorocentrum minimum, Karlodinium veneficum and Gyrodinium instriatum) after exposure to IRI-160AA. In this study, IRI-160AA induced cell cycle arrest in all dinoflagellates examined. Several indicators for programmed cell death (PCD) that are often observed in phytoplankton in response to a variety of stressors were also evaluated. Cell death was accompanied by significant increases in DNA degradation, intra- and extracellular ROS concentrations and DEVDase (caspase-3 like) protease activity, which have been associated with PCD in other phytoplankton species. Overall, results of this investigation provide strong evidence that treatment with the bacterial algicide, IRI-160AA results in cell cycle arrest and induces biochemical changes consistent with stress-related cell death responses observed in other phytoplankton.

  18. Genes and Gene Networks Involved in Sodium Fluoride-Elicited Cell Death Accompanying Endoplasmic Reticulum Stress in Oral Epithelial Cells

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    Yoshiaki Tabuchi

    2014-05-01

    Full Text Available Here, to understand the molecular mechanisms underlying cell death induced by sodium fluoride (NaF, we analyzed gene expression patterns in rat oral epithelial ROE2 cells exposed to NaF using global-scale microarrays and bioinformatics tools. A relatively high concentration of NaF (2 mM induced cell death concomitant with decreases in mitochondrial membrane potential, chromatin condensation and caspase-3 activation. Using 980 probe sets, we identified 432 up-regulated and 548 down-regulated genes, that were differentially expressed by >2.5-fold in the cells treated with 2 mM of NaF and categorized them into 4 groups by K-means clustering. Ingenuity® pathway analysis revealed several gene networks from gene clusters. The gene networks Up-I and Up-II included many up-regulated genes that were mainly associated with the biological function of induction or prevention of cell death, respectively, such as Atf3, Ddit3 and Fos (for Up-I and Atf4 and Hspa5 (for Up-II. Interestingly, knockdown of Ddit3 and Hspa5 significantly increased and decreased the number of viable cells, respectively. Moreover, several endoplasmic reticulum (ER stress-related genes including, Ddit3, Atf4 and Hapa5, were observed in these gene networks. These findings will provide further insight into the molecular mechanisms of NaF-induced cell death accompanying ER stress in oral epithelial cells.

  19. Hypoxia preconditioning of mesenchymal stromal cells enhances PC3 cell lymphatic metastasis accompanied by VEGFR-3/CCR7 activation.

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    Huang, Xin; Su, Kunkai; Zhou, Limin; Shen, Guofang; Dong, Qi; Lou, Yijia; Zheng, Shu

    2013-12-01

    Mesenchymal stromal cells (MSCs) in bone marrow may enhance tumor metastases through the secretion of chemokines. MSCs have been reported to home toward the hypoxic tumor microenvironment in vivo. In this study, we investigated prostate cancer PC3 cell behavior under the influence of hypoxia preconditioned MSCs and explored the related mechanism of prostate cancer lymphatic metastases in mice. Transwell assays revealed that VEGF-C receptor, VEGFR-3, as well as chemokine CCL21 receptor, CC chemokine receptor 7 (CCR7), were responsible for the migration of PC3 cells toward hypoxia preconditioned MSCs. Knock-in Ccr7 in PC3 cells also improved cell migration in vitro. Furthermore, when PC3 cells were labeled using the hrGfp-lentiviral vector, and were combined with hypoxia preconditioned MSCs for xenografting, it resulted in an enhancement of lymph node metastases accompanied by up-regulation of VEGFR-3 and CCR7 in primary tumors. Both PI3K/Akt/IκBα and JAK2/STAT3 signaling pathways were activated in xenografts in the presence of hypoxia-preconditioned MSCs. Unexpectedly, the p-VEGFR-2/VEGFR-2 ratio was attenuated accompanied by decreased JAK1 expression, indicating a switching-off of potential vascular signal within xenografts in the presence of hypoxia-preconditioned MSCs. Unlike results from other studies, VEGF-C maintained a stable expression in both conditions, which indicated that hypoxia preconditioning of MSCs did not influence VEGF-C secretion. Our results provide the new insights into the functional molecular events and signalings influencing prostate tumor metastases, suggesting a hopeful diagnosis and treatment in new approaches.

  20. A case of Fanconi syndrome accompanied by crystal depositions in tubular cells in a patient with multiple myeloma

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    Do Hee Kim

    2014-06-01

    Full Text Available Fanconi syndrome (FS is a rare condition that is characterized by defects in the proximal tubular function. A 48-year-old woman was admitted for evaluation of proteinuria. The patient showed normal anion gap acidosis, normoglycemic glycosuria, hypophosphatemia, and hypouricemia. Thus, her condition was compatible with FS. The M peak was found behind the beta globulin region in urine protein electrophoresis. Upon bone marrow examination, we found that 24% of cells were CD138+ plasma cells with kappa restriction. From a kidney biopsy, we found crystalline inclusions within proximal tubular epithelial cells. Thereafter, she was diagnosed with FS accompanied by multiple myeloma. The patient received chemotherapy and autologous stem cell transplantation, and obtained very good partial hematologic response. However, proximal tubular dysfunction was persistent until 1 year after autologous stem cell transplantation. In short, we report a case of FS accompanied by multiple myeloma, demonstrating crystalline inclusion in proximal tubular cells on kidney biopsy.

  1. Increased genome instability is not accompanied by sensitivity to DNA damaging agents in aged yeast cells

    NARCIS (Netherlands)

    Novarina, Daniele; Mavrova, Sara N.; Janssens, Georges E.; Rempel, Irina L.; Veenhoff, Liesbeth M.; Chang, Michael

    The budding yeast Saccharomyces cerevisiae divides asymmetrically, producing a new daughter cell from the original mother cell. While daughter cells are born with a full lifespan, a mother cell ages with each cell division and can only generate on average 25 daughter cells before dying. Aged yeast

  2. Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells

    OpenAIRE

    Jen-Yu Hung; Ching-Wen Wen; Ya-Ling Hsu; En-Shyh Lin; Ming-Shyan Huang; Chung-Yi Chen; Po-Lin Kuo

    2013-01-01

    This study investigated the anticancer effects of subamolide A (Sub-A), isolated from Cinnamomum subavenium, on human nonsmall cell lung cancer cell lines A549 and NCI-H460. Treatment of cancer cells with Sub-A resulted in decreased cell viability of both lung cancer cell lines. Sub-A induced lung cancer cell death by triggering mitotic catastrophe with apoptosis. It triggered oxidant stress, indicated by increased cellular reactive oxygen species (ROS) production and decreased glutathione le...

  3. [The role of T-regulatory cells in the pathogenesis of immunological disturbances accompanying obesity and atherosclerosis].

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    Luczyński, Włodzimierz; Bossowski, Artur; Głowińska-Olszewska, Barbara; Kos, Justyna; Stasiak-Barmuta, Anna

    2010-03-30

    Obesity and atherosclerosis, and their consequences, including cardiovascular disease, are plagues of the 21st century. Chronic inflammation, whose mechanism is not well understood, underlies the pathophysiological bases of both processes. T lymphocytes, macrophages, and the proinflammatory cytokines produced by these cells play key roles in the immunological disturbances accompanying obesity and atherosclerosis. It was recently shown that T-regulatory cells can play a role in these processes. T-regulatory cells are a small subpopulation of T cells which are responsible for inhibition of the immune response. In this review, experiments conducted in mice and human models on the role of diminished number and/or function of T-regulatory lymphocytes in the pathogenesis of immune disturbances accompanying obesity and atherosclerosis are discussed. The results of studies using T-regulatory cells to stabilize and decrease atherosclerotic lesions in blood vessel walls are also summarized. The results of experiments performed so far are encouraging and give some hope for the future use of T-regulatory cells in the therapy of obesity and atherosclerosis.

  4. The role of T-regulatory cells in the pathogenesis of immunological disturbances accompanying obesity and atherosclerosis

    Directory of Open Access Journals (Sweden)

    Włodzimierz Łuczyński

    2010-03-01

    Full Text Available Obesity and atherosclerosis, and their consequences, including cardiovascular disease, are plagues of the 21st century. Chronic inflammation, whose mechanism is not well understood, underlies the pathophysiological bases of both processes. T lymphocytes, macrophages, and the proinflammatory cytokines produced by these cells play key roles in the immunological disturbances accompanying obesity and atherosclerosis. It was recently shown that T-regulatory cells can play a role in these processes. T-regulatory cells are a small subpopulation of T cells which are responsible for inhibition of the immune response. In this review, experiments conducted in mice and human models on the role of diminished number and/or function of T-regulatory lymphocytes in the pathogenesis of immune disturbances accompanying obesity and atherosclerosis are discussed. The results of studies using T-regulatory cells to stabilize and decrease atherosclerotic lesions in blood vessel walls are also summarized. The results of experiments performed so far are encouraging and give some hope for the future use of T-regulatory cells in the therapy of obesity and atherosclerosis.

  5. Subamolide a induces mitotic catastrophe accompanied by apoptosis in human lung cancer cells.

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    Hung, Jen-Yu; Wen, Ching-Wen; Hsu, Ya-Ling; Lin, En-Shyh; Huang, Ming-Shyan; Chen, Chung-Yi; Kuo, Po-Lin

    2013-01-01

    This study investigated the anticancer effects of subamolide A (Sub-A), isolated from Cinnamomum subavenium, on human nonsmall cell lung cancer cell lines A549 and NCI-H460. Treatment of cancer cells with Sub-A resulted in decreased cell viability of both lung cancer cell lines. Sub-A induced lung cancer cell death by triggering mitotic catastrophe with apoptosis. It triggered oxidant stress, indicated by increased cellular reactive oxygen species (ROS) production and decreased glutathione level. The elevated ROS triggered the activation of ataxia-telangiectasia mutation (ATM), which further enhanced the ATF3 upregulation and subsequently enhanced p53 function by phosphorylation at Serine 15 and Serine 392. The antioxidant, EUK8, significantly decreased mitotic catastrophe by inhibiting ATM activation, ATF3 expression, and p53 phosphorylation. The reduction of ATM and ATF3 expression by shRNA decreased Sub-A-mediated p53 phosphorylation and mitotic catastrophe. Sub-A also caused a dramatic 70% reduction in tumor size in an animal model. Taken together, cell death of lung cancer cells in response to Sub-A is dependent on ROS generation, which triggers mitotic catastrophe followed by apoptosis. Therefore, Sub-A may be a novel anticancer agent for the treatment of nonsmall cell lung cancer.

  6. Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells

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    Jen-Yu Hung

    2013-01-01

    Full Text Available This study investigated the anticancer effects of subamolide A (Sub-A, isolated from Cinnamomum subavenium, on human nonsmall cell lung cancer cell lines A549 and NCI-H460. Treatment of cancer cells with Sub-A resulted in decreased cell viability of both lung cancer cell lines. Sub-A induced lung cancer cell death by triggering mitotic catastrophe with apoptosis. It triggered oxidant stress, indicated by increased cellular reactive oxygen species (ROS production and decreased glutathione level. The elevated ROS triggered the activation of ataxia-telangiectasia mutation (ATM, which further enhanced the ATF3 upregulation and subsequently enhanced p53 function by phosphorylation at Serine 15 and Serine 392. The antioxidant, EUK8, significantly decreased mitotic catastrophe by inhibiting ATM activation, ATF3 expression, and p53 phosphorylation. The reduction of ATM and ATF3 expression by shRNA decreased Sub-A-mediated p53 phosphorylation and mitotic catastrophe. Sub-A also caused a dramatic 70% reduction in tumor size in an animal model. Taken together, cell death of lung cancer cells in response to Sub-A is dependent on ROS generation, which triggers mitotic catastrophe followed by apoptosis. Therefore, Sub-A may be a novel anticancer agent for the treatment of nonsmall cell lung cancer.

  7. Anisomycin-induced GATA-6 degradation accompanying a decrease of proliferation of colorectal cancer cell

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    Ushijima, Hironori; Horyozaki, Akiko; Maeda, Masatomo, E-mail: mmaeda@nupals.ac.jp

    2016-09-09

    Transcription factor GATA-6 plays a key role in normal cell differentiation of the mesoderm and endoderm. On the other hand, GATA-6 is abnormally overexpressed in many clinical gastrointestinal cancer tissue samples, and accelerates cell proliferation or an anti-apoptotic response in cancerous tissues. We previously showed that activation of the JNK signaling cascade causes proteolysis of GATA-6. In this study, we demonstrated that anisomycin, a JNK activator, stimulates nuclear export of GATA-6 in a colorectal cancer cell line, DLD-1. Concomitantly, anisomycin remarkably inhibits the proliferation of DLD-1 cells via G2/M arrest in a plate culture. However, it did not induce apoptosis under growth arrest conditions. Furthermore, the growth of DLD-1 cells in a spheroid culture was suppressed by anisomycin. Although 5-FU showed only a slight inhibitory effect on 3D spheroid cultures, the same concentration of 5-FU together with a low concentration of anisomycin exhibited strong growth inhibition. These results suggest that the induction of GATA-6 dysfunction may be more effective for chemotherapy for colorectal cancer, although the mechanism underlying the synergistic effect of 5-FU and anisomycin remains unknown. - Highlights: • Anisomycin induces proteolysis of GATA-6 in DLD-1 cells. • Anisomycin remarkably inhibits the proliferation of DLD-1 cells via G2/M arrest. • Anisomycin suppresses the growth of spheroids of DLD-1, and enhances the effect of 5-FU.

  8. Caspase-like activities accompany programmed cell death events in developing barley grains.

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    Van Tran

    Full Text Available Programmed cell death is essential part of development and cell homeostasis of any multicellular organism. We have analyzed programmed cell death in developing barley caryopsis at histological, biochemical and molecular level. Caspase-1, -3, -4, -6 and -8-like activities increased with aging of pericarp coinciding with abundance of TUNEL positive nuclei and expression of HvVPE4 and HvPhS2 genes in the tissue. TUNEL-positive nuclei were also detected in nucellus and nucellar projection as well as in embryo surrounding region during early caryopsis development. Quantitative RT-PCR analysis of micro-dissected grain tissues revealed the expression of HvVPE2a, HvVPE2b, HvVPE2d, HvPhS2 and HvPhS3 genes exclusively in the nucellus/nucellar projection. The first increase in cascade of caspase-1, -3, -4, -6 and -8-like activities in the endosperm fraction may be related to programmed cell death in the nucellus and nucellar projection. The second increase of all above caspase-like activities including of caspase-9-like was detected in the maturating endosperm and coincided with expression of HvVPE1 and HvPhS1 genes as well as with degeneration of nuclei in starchy endosperm and transfer cells. The distribution of the TUNEL-positive nuclei, tissues-specific expression of genes encoding proteases with potential caspase activities and cascades of caspase-like activities suggest that each seed tissue follows individual pattern of development and disintegration, which however harmonizes with growth of the other tissues in order to achieve proper caryopsis development.

  9. Sterile subperiosteal fluid collections accompanying orbital wall infarction in sickle-cell disease.

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    Huckfeldt, Rachel M; Shah, Ankoor S

    2014-10-01

    Infarction of the orbital wall is an uncommon manifestation of sickle cell disease (SCD) that may mimic an infectious process. We report a patient with two separate orbital infarctions with different presenting symptoms involving different bones. Radiologic-guided sampling of a periosteal fluid collection in the first episode showed likely sterile inflammatory exudates. This case highlights the range of findings in orbital wall infarction in SCD as well as helpful clinical and imaging entities that may differentiate infarction from infection, allowing early diagnosis and appropriate management.

  10. Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells

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    Barbora Brodská

    2014-01-01

    Full Text Available While p53-dependent apoptosis is triggered by combination of methyltransferase inhibitor decitabine (DAC and histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA in leukemic cell line CML-T1, reactive oxygen species (ROS generation as well as survivin and Bcl-2 deregulation participated in DAC + SAHA-induced apoptosis in p53-deficient HL-60 cell line. Moreover, decrease of survivin expression level is accompanied by its delocalization from centromere-related position in mitotic cells suggesting that both antiapoptotic and cell cycle regulation roles of survivin are affected by DAC + SAHA action. Addition of subtoxic concentration of all-trans-retinoic acid (ATRA increases the efficiency of DAC + SAHA combination on viability, apoptosis induction, and ROS generation in HL-60 cells but has no effect in CML-T1 cell line. Peripheral blood lymphocytes from healthy donors showed no damage induced by DAC + SAHA + ATRA combination. Therefore, combination of ATRA with DAC and SAHA represents promising tool for therapy of leukemic disease with nonfunctional p53 signalization.

  11. A Case of Malignant T-Cell Lymphoma of Gastric Origin Accompanied by Pyothorax

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    Naoki Asakage

    2009-06-01

    Full Text Available The patient was a 74-year-old man suffering from tuberculotic chronic pyothorax. He had hematemesis in January 2006. Hb was 6.1 g/dl. A type 2 tumor 3 cm in diameter was found in the vaulted region on the greater curvature side. It was diagnosed as a malignant lymphoma. WBC and differential count were normal, and the patient tested negative for HTVL-1 antibody. sIL2-R was elevated to 1,500 U/ml. The superficial lymph nodes were not palpable. CT examination was not remarkable for the liver and spleen. There was no generalized lymph node enlargement. Based on these findings, a diagnosis of malignant lymphoma of gastric origin was made. As the patient had respiratory disorders, too, wedge-shaped gastrectomy was performed to inhibit invasion. Pathological examination revealed CD3 positive large atypical lymphocytes diffusely, EBV positive, HP negative. As a result, a diagnosis of non-Hodgkin T-cell lymphoma was made. The tumor did not return for 1 year and 8 months after surgery, but the patient died of sudden aggravation of respiratory disorders in September 2007. Pathological anatomy was performed. The gastric remnant was left with lymphoma, and the bone marrow and systemic lymph nodes were negative for a malignant lymphoma. The possibility of stomach metastasis from the preoperative pyothorax-related malignant lymphoma was considered, but was ruled out because the lungs were devoid of a malignant lymphoma. We report a case of an extremely rare malignant T-cell lymphoma of gastric origin.

  12. Ultrastructural and cytochemical identification of apoptotic cell death accompanying development of the fetal rat olfactory nerve layer.

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    Pellier, V; Saucier, D; Oestreicher, A B; Astic, L

    1996-07-01

    It has been previously shown that the embryonic olfactory nerve contains, in addition to glial ensheathing cells, a large population of differentiated neurons that migrate from the developing olfactory epithelium, in close association with the olfactory axon fascicles. The purpose of our study was to verify the hypothesis according to which a process of physiological cell death might be involved in the progressive disappearance of these migrating neurons that has been reported during late embryonic stages in several immunocytochemical studies. To do so, we have investigated the development of the olfactory nerve layer in rat embryos by using light and electron microscopy, with special reference to the presence of cell death processes within this structure. We have also applied the histochemical TUNEL method allowing in situ visualization of cells degenerating by apoptosis. In order to determine if neurons were present among dying cells, a procedure of double-labeling was performed by combining the DNA-specific bisbenzimide with two neuronal markers, the protein B-50/GAP-43 and the lectin Ulex europaeus I. Results brought out the precise temporal and spatial patterns of programmed cell death accompanying the morphogenesis of the olfactory nerve layer. A cell death process was observed within the olfactory nerve layer from its onset at embryonic day 13 (E13). While only few pycnotic cells were observed in E13 and E14 embryos, their number increased from E15 to reach a maximum at E16 and then diminished. Few dying cells were also observed along the olfactory axon fascicles when they penetrated the olfactory nerve layer. Degenerating cells appeared strongly TUNEL-labeled and exhibited morphological features of cell death by apoptosis. Double-labeling experiments revealed that some of the apoptotic cells were neurons. These observations indicate that apoptosis may account for the progressive decrease in the number of migrating neurons present within the embryonic

  13. Langerhans Cell Histiocytosis of the Thyroid with Multiple Cervical Lymph Node Involvement Accompanying Metastatic Thyroid Papillary Carcinoma

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    Ceyran, A. Bahar; Şenol, Serkan; Bayraktar, Barış; Özkanlı, Şeyma; Cinel, Z. Leyla; Aydın, Abdullah

    2014-01-01

    A 37-year-old male case was admitted with goiter. Ultrasonography of thyroid showed a 5 cm cystic nodule in the left lobe with a 1.5 cm solid component. Fine needle aspiration biopsy revealed atypia of undetermined significance or follicular lesion. The patient was operated on. The pathological diagnosis was reported as papillary thyroid carcinoma. The immunohistochemical examination showed multiple foci of Langerhans cell histiocytosis involving both lobes. The patient died due to cardiac arrest with respiratory causes in the early postoperative period. Langerhans cell histiocytosis is a rare primary condition which involves abnormal clonal proliferation of Langerhans cells in various tissues and organs. Thyroid involvement is infrequently seen. Although the etiology is unknown, genetic components may be linked to the disease. It is also associated with a family history of thyroid disease. Papillary thyroid carcinoma is the most common malignant epithelial tumor of the thyroid gland. Langerhans cell histiocytosis presenting with papillary thyroid carcinoma is rare. The privilege of our case is langerhans cell histiocytosis of the thyroid with multiple cervical lymph node involvement accompanying cervical lymph node metastatic thyroid papillary carcinoma. PMID:25349760

  14. NMDA receptor antagonist prevents cell death in the hippocampal dentate gyrus induced by hyponatremia accompanying adrenal insufficiency in rats.

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    Izumida, Hisakazu; Takagi, Hiroshi; Fujisawa, Haruki; Iwata, Naoko; Nakashima, Kohtaro; Takeuchi, Seiji; Iwama, Shintaro; Namba, Takashi; Komatu, Yukio; Kaibuchi, Kozo; Oiso, Yutaka; Arima, Hiroshi; Sugimura, Yoshihisa

    2017-01-01

    Selective apoptosis of granule cells in the hippocampal dentate gyrus (DG) of rats with bilateral adrenalectomy (ADX) and in patients who died of adrenal insufficiency has been reported. Although adrenal insufficiency is a common disease and is usually associated with hyponatremia, its effect on the central nervous system and in apoptosis in the hippocampus remain to be elucidated. Using rat models to represent clinical hyponatremia accompanying adrenal insufficiency, we show that reduced serum [Na(+)] was associated with selective apoptosis in the DG. Nine days after ADX, apoptotic cells were observed in the DG of rats whose serum [Na(+)] was <125mEq/L (moderate hyponatremia), but rarely in those whose serum [Na(+)] was ≥125mEq/L or in normonatremic rats. Although all hyponatremic ADX rats survived following treatment with corticosterone and saline started 7days after ADX when apoptosis had not yet occurred, selective apoptosis on day 9 was not prevented in moderately hyponatremic rats. Interestingly, treatment with memantine, a noncompetitive NMDAR antagonist, prevented the selective apoptosis in the DG in moderately hyponatremic, ADX rats, and improved electrophysiological dysfunction, including impaired basal synaptic transmission and long-term potentiation at the entorhinal cortex-DG synapses. These results demonstrated that in adrenal insufficient rats, hyponatremia was associated with apoptosis in the DG, and that memantine prevented the apoptosis and improved cell function. Our data imply the importance of assessing the possibility of neurological impairments after treatment with CORT in patients with moderate or severe hyponatremia accompanying adrenal insufficiency and that memantine may represent a beneficial therapeutic strategy to prevent neurological impairments in such patients. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Peripheral T-cell lymphoma, not otherwise specified accompanied by central nervous system involvement with features of lymphomatosis cerebri.

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    Fuseya, Hoyuri; Nakao, Takafumi; Hashimura, Mitsuharu; Horiuchi, Mirei; Hayashi, Yoshiki; Hagihara, Kiyoyuki; Kanashima, Hiroshi; Okuno, Takahiro; Fukushima, Hiroko; Inoue, Takeshi; Manabe, Takao; Yamane, Takahisa

    Lymphomatosis cerebri (LC) is a rare variant of primary central nervous system lymphoma, and it is characterized by diffuse cerebral infiltration of malignant lymphoma cells without evidence of a mass lesion. Herein, we report a patient with systemic peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) who had central nervous system involvement mimicking LC. A 72-year-old immunocompetent male presented with rapidly progressive dementia. Fluor-deoxy-glucose (FDG) -positron emission tomography revealed increased FDG uptake in the bone and skin. Histopathological examination of the skin lesion revealed PTCL-NOS infiltration. A FLAIR MRI scan of the brain revealed diffuse hyperintense lesions in the cerebral white matter of both hemispheres. These lesions were not enhanced with gadolinium, and there was no perceptible mass effect. We performed a brain biopsy, and the histology results were consistent with PTCL-NOS. The patient was treated with corticosteroid and chemotherapy; however the disease progressed, and he died 4 months after the diagnosis. This was a rare case of systemic lymphoma accompanied with central nervous system involvement mimicking LC.

  16. Fisetin and hesperetin induced apoptosis and cell cycle arrest in chronic myeloid leukemia cells accompanied by modulation of cellular signaling.

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    Adan, Aysun; Baran, Yusuf

    2016-05-01

    Fisetin and hesperetin, naturally occurring flavonoids, have been reported as novel antioxidants with chemopreventive/chemotherapeutic potential against various types of cancer. However, their mechanism of action in CML is still unknown. This particular study aims to evaluate the therapeutic potentials of fisetin and hesperetin and their effects on cell proliferation, apoptosis, and cell cycle progression in human K562 CML cells. The results indicated that fisetin and hesperetin inhibited cell proliferation and triggered programmed cell death in these cells. The latter was confırmed by mitochondrial membrane depolarization and an increase in caspase-3 activation. In addition to that, we have detected S and G2/M cell cycle arrests and G0/G1 arrest upon fisetin and hesperetin treatment, respectively. To identify the altered genes and genetic networks in response to fisetin and hesperetin, whole-genome microarray analysis was performed. The microarray gene profiling analysis revealed some important signaling pathways including JAK/STAT pathway, KIT receptor signaling, and growth hormone receptor signaling that were altered upon fisetin and hesperetin treatment. Moreover, microarray data suggested potential candidate genes for targeted CML therapy. Fisetin and hesperetin significantly modulated the expression of genes involved in cell proliferation and division, apoptosis, cell cycle regulation, and other significant cellular processes such as replication, transcription, and translation. In conclusion, our results suggest that fisetin and hesperetin as potential natural agents for CML therapy.

  17. ATM activation accompanies histone H2AX phosphorylation in A549 cells upon exposure to tobacco smoke

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    Traganos Frank

    2007-06-01

    Full Text Available Abstract Background In response to DNA damage or structural alterations of chromatin, histone H2AX may be phosphorylated on Ser139 by phosphoinositide 3-kinase related protein kinases (PIKKs such as ataxia telangiectasia mutated (ATM, ATM-and Rad-3 related (ATR kinase, or by DNA dependent protein kinase (DNA-PKcs. When DNA damage primarily involves formation of DNA double-strand breaks (DSBs, H2AX is preferentially phosphorylated by ATM rather than by the other PIKKs. We have recently reported that brief exposure of human pulmonary adenocarcinoma A549 cells or normal human bronchial epithelial cells (NHBE to cigarette smoke (CS induced phosphorylation of H2AX. Results We report here that H2AX phosphorylation in A549 cells induced by CS was accompanied by activation of ATM, as revealed by ATM phosphorylation on Ser1981 (ATM-S1981P detected immunocytochemically and by Western blotting. No cell cycle-phase specific differences in kinetics of ATM activation and H2AX phosphorylation were observed. When cells were exposed to CS from cigarettes with different tobacco and filter combinations, the expression levels of ATM-S1981P correlated well with the increase in expression of phosphorylated H2AX (γH2AX (R = 0.89. In addition, we note that while CS-induced γH2AX expression was localized within discrete foci, the activated ATM was distributed throughout the nucleoplasm. Conclusion These data implicate ATM as the PIKK that phosphorylates H2AX in response to DNA damage caused by CS. Based on current understanding of ATM activation, expression and localization, these data would suggest that, in addition to inducing potentially carcinogenic DSB lesions, CS may also trigger other types of DNA lesions and cause chromatin alterations. As checkpoint kinase (Chk 1, Chk2 and the p53 tumor suppressor gene are known to be phosphorylated by ATM, the present data indicate that exposure to CS may lead to their phosphorylation, with the downstream consequences

  18. Hippocampal cell loss and propagation of abnormal discharges accompanied with the expression of tonic convulsion in the spontaneously epileptic rat.

    Science.gov (United States)

    Hanaya, Ryosuke; Sasa, Masashi; Sugata, Sei; Tokudome, Mai; Serikawa, Tadao; Kurisu, Kaoru; Arita, Kazunori

    2010-04-30

    Spontaneously epileptic rats (SER) are double mutants with both tonic convulsion and absence-like seizures from the age of 8 weeks. Hippocampal CA3 neurons in SER display a long-lasting depolarizing shift accompanied by repetitive firing (attributed to abnormalities of the Ca(2+) channels) with a single stimulation of the mossy fibers. In the present investigation, we examined if the seizure discharges of SER were correlated with the hippocampal abnormality of SER using electrophysiological and histological methods. In CA1 neurons of seizure-susceptible mature SER, higher-voltage (<8-11 V) stimulations induced a long depolarization shift (in 25% of neurons) with repetitive firing (in 12.5% of neurons). However, the tremor rat, one of the parent strains of SER, did not exhibit such abnormal firing in the CA3 region of the hippocampus. The number of CA3 neurons in SER was significantly (p<0.01) lower than that in tremor rats and Wistar rats, although no significant difference was established in the hilus. Sprouting of mossy fiber was observed in the dentate of mature SER; however, negligible staining was spotted in the dentate of both mature tremor and Wistar rats. Interestingly, expression of the brain-derived neurotrophic factor was higher in the hilus, CA3, and granular cell layer of dentate gyrus in SER than normal Wistar rats. The expression levels of TUNEL, bax, and Caspase-3 did not show significant changes between the SER and Wistar rats. SER exhibited hippocampal sclerosis-like changes which did not have enough potential for epileptogenesis. Repetitive tonic seizures and vulnerable CA3 neurons of SER could be involved in the induction of sclerosis-like changes in the hippocampus.

  19. Transition from Preinvasive Carcinoma In Situ to Seminoma Is Accompanied by a Reduction of Connexin 43 Expression in Sertoli Cells and Germ Cells

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    Ralph Brehm

    2006-06-01

    Full Text Available Carcinoma in situ (CIS represents the preinvasive stage of human germ cell tumors, but the mechanism leading to pubertal proliferation and invasive malignancy remains unknown. Among testicular gap functional proteins, connexin 43 (Cx43 represents the predominant Cx, and, previously, an inverse correlation between synthesis of Cx43 protein and progression of tumor development was detected. In the present study, using cDNA microarray analysis, in situ hybridization, semi quantitative reverse transcription-polymerase chain reaction (RT-PCR from tissue homogenates, RT-PCR from microdissected tubules with normal spermatogenesis and CIS, and seminoma cells from invasive seminoma, we asked whether reduction of Cx43 protein is accompanied by a change of Cx43 transcripts. We detected a significant downregulation of Cx43 at mRNA level in Sertoli and germ cells starting in seminiferous tubules infiltrated with CIS and resulting in a complete loss in seminoma cells. It was demonstrated that downregulation of Cx43 expression in neoplastic human testis takes place at the transcriptional level and starts in CIS. This reduction of Cx43 expression further suggests that early intratubular derangement in Cx43 gene expression and disruption of intercellular communication between Sertoli cells and/or Sertoli and preinvasive tumor cells may play a role in the progression phase of human seminoma development.

  20. Transition from Preinvasive Carcinoma In Situ to Seminoma Is Accompanied by a Reduction of Connexin 43 Expression in Sertoli Cells and Germ Cells1

    Science.gov (United States)

    Brehm, Ralph; Rüttinger, Christina; Fischer, Petra; Gashaw, Isabella; Winterhager, Elke; Kliesch, Sabine; Bohle, Rainer M; Steger, Klaus; Bergmann, Martin

    2006-01-01

    Abstract Carcinoma in situ (CIS) represents the preinvasive stage of human germ cell tumors, but the mechanism leading to pubertal proliferation and invasive malignancy remains unknown. Among testicular gap junctional proteins, connexin 43 (Cx43) represents the predominant Cx, and, previously, an inverse correlation between synthesis of Cx43 protein and progression of tumor development was detected. In the present study, using cDNA microarray analysis, in situ hybridization, semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) from tissue homogenates, RT-PCR from microdissected tubules with normal spermatogenesis and CIS, and seminoma cells from invasive seminoma, we asked whether reduction of Cx43 protein is accompanied by a change of Cx43 transcripts. We detected a significant downregulation of Cx43 at mRNA level in Sertoli and germ cells starting in seminiferous tubules infiltrated with CIS and resulting in a complete loss in seminoma cells. It was demonstrated that downregulation of Cx43 expression in neoplastic human testis takes place at the transcriptional level and starts in CIS. This reduction of Cx43 expression further suggests that early intratubular derangement in Cx43 gene expression and disruption of intercellular communication between Sertoli cells and/or Sertoli and preinvasive tumor cells may play a role in the progression phase of human seminoma development. PMID:16820096

  1. Phosphorylation and sulfation of arylsulfatase A accompanies biosynthesis of the enzyme in normal and carcinoma cell lines.

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    Waheed, A; van Etten, R L

    1985-10-30

    Arylsulfatase A (arylsulfate sulfohydrolase, EC 3.1.6.1), a mammalian lysosomal enzyme, is initially synthesized as a 69, 67 and 64 kDa precursor polypeptide in a prostate carcinoma cell line PC-3SF12, in HeLa cells and in a normal human embryonic lung cell line WI-38, respectively. These precursor polypeptides are secreted into the medium or processed to mature enzymes of apparent molecular mass 66, 64 or 62 kDa in PC-3SF12, HeLa or WI-38 cells, respectively. The precursor and mature polypeptides in WI-38 cells are phosphorylated, and the phosphate is lost upon treatment with endo-beta-hexosaminidase H. Arylsulfatase A is also shown to be sulfated in WI-38 cells. The presence of castanospermine, an inhibitor of sulfation of the second N-acetylglucosamine residue of the chitobiose core, does not reduce the extent of sulfation of arylsulfatase A, suggesting that either terminal sugars or the protein is sulfated. Sulfation may have a protective function similar to that of terminal sialic acid residues in glycoproteins. Although the subcellular location of arylsulfatase A is identical in PC-3SF12 and in WI-38 cells, pulse-chase experiments indicate that arylsulfatase A protein has a slower turnover in the prostate carcinoma cell line than it does in the normal human lung cell line. The differences in the apparent molecular weights of arylsulfatase A in the normal and carcinoma cell lines are shown to be due to variations in the carbohydrate content of the enzyme. The apparent molecular mass of the polypeptide chain obtained after endo-beta-hexosaminidase H treatment is 59 kDa, a value which is identical for all three cell lines studied here. These results suggest the possibility of an enhanced activity of terminal glucosyltransferase enzymes in carcinoma cell lines and in tumor tissues. Arylsulfatase A may be a useful marker for studying transformation-related processes in human cell lines.

  2. Lactogenic differentiation of HC11 cells is not accompanied by downregulation of AP-2 transcription factor genes

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    Schorle Hubert

    2008-06-01

    Full Text Available Abstract Background During pregnancy the mammary epithelium undergoes a complex developmental process which culminates in the generation of the milk-secreting epithelium. Secretory epithelial cells display lactogenic differentiation which is characterized by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP. Transcription factors AP-2alpha and AP-2gamma are downregulated during lactation, and their overexpression in transgenic mice impaired the secretory differentiation of the mammary epithelium, resulting in lactation failure. To explore whether the downregulation of AP-2alpha and AP-2gamma is of functional significance for lactogenic differentiation, we analyzed the expression of the AP-2 family members during the lactogenic differentiation of HC11 mammary epithelial cells in vitro. Differentiation of HC11 cells was induced following established protocols by applying the lactogenic hormones prolactin, dexamethasone and insulin. Findings HC11 cells express all AP-2 family members except AP-2delta. Using RT-PCR we could not detect a downregulation of any of these genes during the lactogenic differentiation of HC11 cells in vitro. This finding was confirmed for AP-2alpha and AP-2gamma using Northern analysis. Differentiating HC11 cells displayed lower expression levels of milk protein genes than mammary glands of mid-pregnant or lactating mice. Conclusion The extent of lactogenic differentiation of HC11 cells in vitro is limited compared to mammary epithelium undergoing secretory differentiation in vivo. Downregulation of AP-2 transcription factor genes is not required for lactogenic differentiation of HC11 cells but may functionally be involved in aspects of lactogenic differentiation in vivo that are not reflected by the HC11 system.

  3. Misregulation of Stromelysin-1 in Mouse Mammary Tumor Cells Accompanies Acquisition of Stromelysin-1 dependent Invasive Properties

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    Lochter, A.; Srebrow, A.; Sympson, C.J.; Terracio, N.; Werb, Z.; Bissell, M.J.

    1997-02-21

    Stromelysin-1 is a member of the metalloproteinase family of extracellular matrix-degrading enzymes that regulates tissue remodeling. We previously established a transgenic mouse model in which rat stromelysin-1 targeted to the mammary gland augmented expression of endogenous stromelysin-1, disrupted functional differentiation, and induced mammary tumors. A cell line generated from an adenocarcinoma in one of these animals and a previously described mammary tumor cell line generated in culture readily invaded both a reconstituted basement membrane and type I collagen gels, whereas a nonmalignant, functionally normal epithelial cell line did not. Invasion of Matrigel by tumor cells was largely abolished by metalloproteinase inhibitors, but not by inhibitors of other proteinase families. Inhibition experiments with antisense oligodeoxynucleotides revealed that Matrigel invasion of both cell lines was critically dependent on stromelysin-1 expression. Invasion of collagen, on the other hand, was reduced by only 40-50%. Stromelysin-1 was expressed in both malignant and nonmalignant cells grown on plastic substrata. Its expression was completely inhibited in nonmalignant cells, but up-regulated in tumor cells, in response to Matrigel. Thus misregulation of stromelysin-1 expression appears to be an important aspect of mammary tumor cell progression to an invasive phenotype. The matrix metalloproteinases (MMPs) are a family of extracellular matrix (ECM)-degrading enzymes that have been implicated in a variety of normal developmental and pathological processes, including tumorigenesis. The MMP family comprises at least 15 members with different, albeit overlapping, substrate specificities. During activation of latent MMPs, their propeptides are cleaved and they are converted to a lower molecular weight form by other enzymes, including serine proteinases, and by autocatalytic cleavage. Among the MMPs, stromelysin-1 (SL1) possesses the broadest substrate specificity. Despite

  4. Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli.

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    Krejčí, Jana; Legartová, Soňa; Bártová, Eva

    2017-01-01

    Cajal bodies (CBs) are important compartments containing accumulated proteins that preferentially regulate RNA-related nuclear events, including splicing. Here, we studied the nuclear distribution pattern of CBs in neurogenesis. In adult brains, coilin was present at a high density, but CB formation was absent in the nuclei of the choroid plexus of the lateral ventricles. Cells of the adult hippocampus were characterized by a crescent-like morphology of coilin protein. We additionally observed a 70 kDa splice variant of coilin in adult mouse brains, which was different to embryonic brains and mouse pluripotent embryonic stem cells (mESCs), characterized by the 80 kDa standard variant of coilin. Here, we also showed that depletion of coilin is induced during neural differentiation and HDAC1 deficiency in mESCs caused coilin accumulation inside the fibrillarin-positive region of the nucleoli. A similar distribution pattern was observed in adult brain hippocampi, characterized by lower levels of both coilin and HDAC1. In summary, we observed that neural differentiation and HDAC1 deficiency lead to coilin depletion and coilin accumulation in body-like structures inside the nucleoli.

  5. Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli

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    Jana Krejčí

    2017-01-01

    Full Text Available Cajal bodies (CBs are important compartments containing accumulated proteins that preferentially regulate RNA-related nuclear events, including splicing. Here, we studied the nuclear distribution pattern of CBs in neurogenesis. In adult brains, coilin was present at a high density, but CB formation was absent in the nuclei of the choroid plexus of the lateral ventricles. Cells of the adult hippocampus were characterized by a crescent-like morphology of coilin protein. We additionally observed a 70 kDa splice variant of coilin in adult mouse brains, which was different to embryonic brains and mouse pluripotent embryonic stem cells (mESCs, characterized by the 80 kDa standard variant of coilin. Here, we also showed that depletion of coilin is induced during neural differentiation and HDAC1 deficiency in mESCs caused coilin accumulation inside the fibrillarin-positive region of the nucleoli. A similar distribution pattern was observed in adult brain hippocampi, characterized by lower levels of both coilin and HDAC1. In summary, we observed that neural differentiation and HDAC1 deficiency lead to coilin depletion and coilin accumulation in body-like structures inside the nucleoli.

  6. Mycobacterium tuberculosis PE_PGRS17 Promotes the Death of Host Cell and Cytokines Secretion via Erk Kinase Accompanying with Enhanced Survival of Recombinant Mycobacterium smegmatis

    Science.gov (United States)

    Chen, Tian; Zhao, Quanju; Li, Wu

    2013-01-01

    Tuberculosis (TB) remains a serious threat to global public health, largely due to the successful manipulation of the host immunity by its etiological agent Mycobacterium tuberculosis. The PE_PGRS protein family of M. tuberculosis might be a contributing factor. To investigate the roles of PE_PGRS17, the gene of PE_PGRS 17 was expressed in nonpathogenic fast growing Mycobacterium smegmatis. We found that the recombinant strain survives better than the control in macrophage cultures, accompanied by more host cell death and a marked higher secretion of tumor necrosis factor-alpha by a recombinant strain compared with control. Blocking the action of Erk kinase by an inhibitor can abolish the above effects. In brief, our data showed that PE_PGRS 17 might facilitate pathogen survival and disserve the host cell via remodeling the macrophages immune niche largely consisting of inflammatory cytokines. This furnishes a novel insight into the immune role of this mycobacterium unique gene family. PMID:23663047

  7. Characteristic Changes in Cell Surface Glycosylation Accompany Intestinal Epithelial Cell (IEC) Differentiation: High Mannose Structures Dominate the Cell Surface Glycome of Undifferentiated Enterocytes.

    Science.gov (United States)

    Park, Dayoung; Brune, Kristin A; Mitra, Anupam; Marusina, Alina I; Maverakis, Emanual; Lebrilla, Carlito B

    2015-11-01

    Changes in cell surface glycosylation occur during the development and differentiation of cells and have been widely correlated with the progression of several diseases. Because of their structural diversity and sensitivity to intra- and extracellular conditions, glycans are an indispensable tool for analyzing cellular transformations. Glycans present on the surface of intestinal epithelial cells (IEC) mediate interactions with billions of native microorganisms, which continuously populate the mammalian gut. A distinct feature of IECs is that they differentiate as they migrate upwards from the crypt base to the villus tip. In this study, nano-LC/ESI QTOF MS profiling was used to characterize the changes in glycosylation that correspond to Caco-2 cell differentiation. As Caco-2 cells differentiate to form a brush border membrane, a decrease in high mannose type glycans and a concurrent increase in fucosylated and sialylated complex/hybrid type glycans were observed. At day 21, when cells appear to be completely differentiated, remodeling of the cell surface glycome ceases. Differential expression of glycans during IEC maturation appears to play a key functional role in regulating the membrane-associated hydrolases and contributes to the mucosal surface innate defense mechanisms. Developing methodologies to rapidly identify changes in IEC surface glycans may lead to a rapid screening approach for a variety of disease states affecting the GI tract. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells

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    Akiyama, Tomohiko; Xin, Li; Oda, Mayumi; Sharov, Alexei A.; Amano, Misa; Piao, Yulan; Cadet, J. Scotty; Dudekula, Dawood B.; Qian, Yong; Wang, Weidong; Ko, Shigeru B. H.; Ko, Minoru S. H.

    2015-01-01

    Mouse embryonic stem cells (mESCs) have a remarkable capacity to maintain normal genome stability and karyotype in culture. We previously showed that infrequent bursts of Zscan4 expression (Z4 events) are important for the maintenance of telomere length and genome stability in mESCs. However, the molecular details of Z4 events remain unclear. Here we show that Z4 events involve unexpected transcriptional derepression in heterochromatin regions that usually remain silent. During a Z4 event, we see rapid derepression and rerepression of heterochromatin leading to a burst of transcription that coincides with transient histone hyperacetylation and DNA demethylation, clustering of pericentromeric heterochromatin around the nucleolus, and accumulation of activating and repressive chromatin remodelling complexes. This heterochromatin-based transcriptional activity suggests that mESCs may maintain their extraordinary genome stability at least in part by transiently resetting their heterochromatin. PMID:26324425

  9. Pure red cell aplasia accompanied by autoimmune hemolytic anemia in a patient with type A viral hepatitis.

    Science.gov (United States)

    Koiso, Hiromi; Kobayashi, Satsuki; Ueki, Kazue; Hamada, Tetsuya; Tsukamoto, Norifumi; Karasawa, Masamitsu; Murakami, Hirokazu; Nojima, Yoshihisa

    2009-05-01

    A rare case of acute hepatitis A associated with autoimmune hemolytic anemia (AIHA) and pure red cell aplasia (PRCA) is reported. A 55-year-old woman consulted a doctor because of common cold-like symptoms and she was referred to our hospital in January 2007. Laboratory findings showed a marked elevation of serum transaminase and total bilirubin levels (AST 9,605 IU/l, ALT 5,546 IU/l and T-bil 4.14 mg/dl), and prolonged prothrombin time, findings which suggested the risk of progression to fulminant hepatitis, and she was treated with plasmapheresis and hemodialysis filtration on the first and second hospital days. She was diagnosed with severe acute hepatitis A based on the elevation of serum IgM anti-hepatitis A virus. On the 20th hospital day, her hemoglobin level began to decrease in spite of improving transaminase levels without any signs of gastrointestinal bleeding. Bilirubin and LDH elevation, haptoglobin decline and a positive direct Coombs test were detected and these findings indicated AIHA complication; however, the reticulocyte count decreased and bone marrow showed marked erythroid hypoplasia so the co-existence of PRCA was diagnosed. After oral prednisolone administration (1 mg/kg/day), her hemolytic anemia rapidly improved.

  10. Overexpression of CD44 accompanies acquired tamoxifen resistance in MCF7 cells and augments their sensitivity to the stromal factors, heregulin and hyaluronan

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    Hiscox Stephen

    2012-10-01

    Full Text Available Abstract Background Acquired resistance to endocrine therapy in breast cancer is a significant problem with relapse being associated with local and/or regional recurrence and frequent distant metastases. Breast cancer cell models reveal that endocrine resistance is accompanied by a gain in aggressive behaviour driven in part through altered growth factor receptor signalling, particularly involving erbB family receptors. Recently we identified that CD44, a transmembrane cell adhesion receptor known to interact with growth factor receptors, is upregulated in tamoxifen-resistant (TamR MCF7 breast cancer cells. The purpose of this study was to explore the consequences of CD44 upregulation in an MCF7 cell model of acquired tamoxifen resistance, specifically with respect to the hypothesis that CD44 may influence erbB activity to promote an adverse phenotype. Methods CD44 expression in MCF7 and TamR cells was assessed by RT-PCR, Western blotting and immunocytochemistry. Immunofluorescence and immunoprecipitation studies revealed CD44-erbB associations. TamR cells (± siRNA-mediated CD44 suppression or MCF7 cells (± transfection with the CD44 gene were treated with the CD44 ligand, hyaluronon (HA, or heregulin and their in vitro growth (MTT, migration (Boyden chamber and wound healing and invasion (Matrigel transwell migration determined. erbB signalling was assessed using Western blotting. The effect of HA on erbB family dimerisation in TamR cells was determined by immunoprecipitation in the presence or absence of CD44 siRNA. Results TamR cells overexpressed CD44 where it was seen to associate with erbB2 at the cell surface. siRNA-mediated suppression of CD44 in TamR cells significantly attenuated their response to heregulin, inhibiting heregulin-induced cell migration and invasion. Furthermore, TamR cells exhibited enhanced sensitivity to HA, with HA treatment resulting in modulation of erbB dimerisation, ligand-independent activation of erbB2

  11. Acute Exacerbations of Chronic Hepatitis B Are Accompanied by Decline of Core Antigen-Specific Regulatory T-Cell Frequencies: Implications for Successful Anti-HBV Treatments

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    Sun-Lung Tsai

    2007-11-01

    Full Text Available Background and Aims: Acute exacerbations (AEs of perinatally-acquired chronic hepatitis B (CHB are accompanied by increased T cell responses to hepatitis B core and e antigens (HBcAg & HBeAg. Naturally-arising forkhead transcription factor Foxp3 (forkhead box p3-expressing CD4+CD25+ regulatory T (Treg cells are thought to be important in the control of infectious diseases. This study aimed to investigate whether HBcAg-specific Treg cells play a role in modulating spontaneous AEs and in influencing the outcome of anti-hepatitis B virus (HBV treatments.Methods: The SYFPEITHI scoring system was employed to predict epitope peptides on HBcAg overlapping with HBeAg for the construction of peptide-HLA class II tetramers to measure HBcAg-specific Treg cell frequencies (Treg f . Results: HBcAg-specific Treg f declined significantly in association with increased HBcAg-specific cytotoxic T lymphocyte frequencies during spontaneous AEs without treatment. Vigorous in vitro expansion of CD4+CD25+ Treg cells from CHB patients responding to HBcAg and/or its peptides plus interleukin-2 (IL-2 was consistently detected. Depletion of Treg cells from peripheral blood mononuclear cells enhanced proliferation to HBcAg. In contrast, patients with AEs who received anti-HBV treatments with oral nucleoside analogues or interferon-alpha injection revealed that more post-treatment increase of HBcAg-specific Treg f correlated with a higher sustained remission rate to the therapy.Conclusions: These data indicate that HBcAg-specific Treg cells from perinatally-acquired CHB patients are proliferative to HBcAg and its peptides and exhibit suppressor activity. They play a crucial role in modulating spontaneous AEs and in successful anti-HBV treatments.

  12. Dehydration induced loss of photosynthesis in Arabidopsis leaves during senescence is accompanied by the reversible enhancement in the activity of cell wall β-glucosidase.

    Science.gov (United States)

    Patro, Lichita; Mohapatra, Pranab Kishor; Biswal, Udaya Chand; Biswal, Basanti

    2014-08-01

    The physiology of loss of photosynthetic production of sugar and the consequent cellular sugar reprogramming during senescence of leaves experiencing environmental stress largely remains unclear. We have shown that leaf senescence in Arabidopsis thaliana causes a significant reduction in the rate of oxygen evolution and net photosynthetic rate (Pn). The decline in photosynthesis is further aggravated by dehydration. During dehydration, primary photochemical reaction of thylakoids and net photosynthesis decrease in parallel with the increase in water deficit. Senescence induced loss in photosynthesis is accompanied by a significant increase in the activity of cell wall hydrolyzing enzyme such as β-glucosidase associated with cell wall catabolism. The activity of this enzyme is further enhanced when the senescing leaves experience dehydration stress. It is possible that both senescence and stress separately or in combination result in the loss in photosynthesis which could be a signal for an enhancement in the activity of β-glucosidase that breaks down cell wall polysaccharides to sugar to sustain respiration for metabolic activities of plants experiencing stress. Thus dehydration response of cell wall hydrolases of senescing leaves is considered as plants' strategy to have cell wall polysaccharides as an alternative energy source for completion of energy requiring senescence process, stress survival and maintenance of recovery potential of energy deficit cells in the background of loss in photosynthesis. Withdrawal of stress (rehydration) distinctly exhibits recovery of photosynthesis and suppression of enzyme activity. Retention of the signaling for sugar reprogramming through breakdown of cell wall polysaccharides in the senescing leaves exposed to severe drought stress suggests that senescing leaves like mature ones possess potential for stress recovery. The precise mechanism of stress adaptation of senescing leaves is yet to be known. A significant

  13. Acquired resistance of pancreatic cancer cells to treatment with gemcitabine and HER-inhibitors is accompanied by increased sensitivity to STAT3 inhibition.

    Science.gov (United States)

    Ioannou, Nikolaos; Seddon, Alan M; Dalgleish, Angus; Mackintosh, David; Solca, Flavio; Modjtahedi, Helmout

    2016-03-01

    Drug-resistance is a major contributing factor for the poor prognosis in patients with pancreatic cancer. We have shown previously that the irreversible ErbB family blocker afatinib, is more effective than the reversible EGFR tyrosine kinase inhibitor erlotinib in inhibiting the growth of human pancreatic cancer cells. The aim of this study was to develop human pancreatic cancer cell (BxPc3) variants with acquired resistance to treatment with gemcitabine, afatinib, or erlotinib, and to investigate the molecular changes that accompany the acquisition of a drug-resistant phenotype. We also investigated the therapeutic potential of various agents in the treatment of such drug-resistant variants. Three variant forms of BxPc3 cells with acquired resistance to gemcitabine (BxPc3GEM), afatinib (BxPc3AFR) or erlotinib (BxPc3OSIR) were developed following treatment with increasing doses of such drugs. The expression level, mutational and phosphorylation status of various growth factor receptors and downstream cell signaling molecules were determined by FACS, human phopsho-RTK array, and western blot analysis while the sulforhodamine B assay was used for determining the effect of various agents on the growth of such tumours. We found that all three BxPc3 variants with acquired resistance to gemcitabine (BxPc3GEM), afatinib (BxPc3AFR) or erlotinib (BxPc3OSIR) also become less sensitive to treatment with the two other agents. Acquisition of resistance to these agents was accompanied by upregulation of p-c-MET, p-STAT3, CD44, increased autocrine production of EGFR ligand amphiregulin and differential activation status of EGFR tyrosine residues as well as downregulation of total and p-SRC. Of all therapeutic interventions examined, including the addition of an anti-EGFR antibody ICR62, an anti-CD44 monoclonal antibody, and of STAT3 or c-MET inhibitors, only treatment with the STAT3 inhibitor Stattic produced a higher growth inhibitory effect in all three drug-resistant variants

  14. Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency

    Science.gov (United States)

    Lee, Kiho; Kwon, Deug-Nam; Ezashi, Toshihiko; Choi, Yun-Jung; Park, Chankyu; Ericsson, Aaron C.; Brown, Alana N.; Samuel, Melissa S.; Park, Kwang-Wook; Walters, Eric M.; Kim, Dae Young; Kim, Jae-Hwan; Franklin, Craig L.; Murphy, Clifton N.; Roberts, R. Michael; Prather, Randall S.; Kim, Jin-Hoi

    2014-01-01

    Pigs with severe combined immunodeficiency (SCID) may provide useful models for regenerative medicine, xenotransplantation, and tumor development and will aid in developing therapies for human SCID patients. Using a reporter-guided transcription activator-like effector nuclease (TALEN) system, we generated targeted modifications of recombination activating gene (RAG) 2 in somatic cells at high efficiency, including some that affected both alleles. Somatic-cell nuclear transfer performed with the mutated cells produced pigs with RAG2 mutations without integrated exogenous DNA. Biallelically modified pigs either lacked a thymus or had one that was underdeveloped. Their splenic white pulp lacked B and T cells. Under a conventional housing environment, the biallelic RAG2 mutants manifested a “failure to thrive” phenotype, with signs of inflammation and apoptosis in the spleen compared with age-matched wild-type animals by the time they were 4 wk of age. Pigs raised in a clean environment were healthier and, following injection of human induced pluripotent stem cells (iPSCs), quickly developed mature teratomas representing all three germ layers. The pigs also tolerated grafts of allogeneic porcine trophoblast stem cells. These SCID pigs should have a variety of uses in transplantation biology. PMID:24799706

  15. Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency.

    Science.gov (United States)

    Lee, Kiho; Kwon, Deug-Nam; Ezashi, Toshihiko; Choi, Yun-Jung; Park, Chankyu; Ericsson, Aaron C; Brown, Alana N; Samuel, Melissa S; Park, Kwang-Wook; Walters, Eric M; Kim, Dae Young; Kim, Jae-Hwan; Franklin, Craig L; Murphy, Clifton N; Roberts, R Michael; Prather, Randall S; Kim, Jin-Hoi

    2014-05-20

    Pigs with severe combined immunodeficiency (SCID) may provide useful models for regenerative medicine, xenotransplantation, and tumor development and will aid in developing therapies for human SCID patients. Using a reporter-guided transcription activator-like effector nuclease (TALEN) system, we generated targeted modifications of recombination activating gene (RAG) 2 in somatic cells at high efficiency, including some that affected both alleles. Somatic-cell nuclear transfer performed with the mutated cells produced pigs with RAG2 mutations without integrated exogenous DNA. Biallelically modified pigs either lacked a thymus or had one that was underdeveloped. Their splenic white pulp lacked B and T cells. Under a conventional housing environment, the biallelic RAG2 mutants manifested a "failure to thrive" phenotype, with signs of inflammation and apoptosis in the spleen compared with age-matched wild-type animals by the time they were 4 wk of age. Pigs raised in a clean environment were healthier and, following injection of human induced pluripotent stem cells (iPSCs), quickly developed mature teratomas representing all three germ layers. The pigs also tolerated grafts of allogeneic porcine trophoblast stem cells. These SCID pigs should have a variety of uses in transplantation biology.

  16. Tetrandrine Induces Apoptosis of Human Nasopharyngeal Carcinoma NPC-TW 076 Cells through Reactive Oxygen Species Accompanied by an Endoplasmic Reticulum Stress Signaling Pathway

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    Ya-Jing Lin

    2016-10-01

    Full Text Available Nasopharyngeal carcinoma (NPC is an epithelial malignancy of the head and neck and the incidence is higher in Southeast Asia. Tetrandrine (TET is a bisbenzylisoquinoline alkaloid, a natural product, and exhibits biological activities including action against many human cancer cell lines. However, the molecular mechanism of TET-induced cell apoptosis in human NPC cells is still unclear. In the present study, we investigated TET-induced apoptotic cell death and associated possible signal pathways on human nasopharyngeal carcinoma NPC-TW 076 cells in vitro. Phase contrast microscopy was used to examine cell morphology and DAPI staining was used to examine chromatin condensation. Flow cytometry assay was used to measure total viable cells, cell cycle and sub-G1 phase distribution, reactive oxygen species (ROS, Ca2+, and mitochondria membrane potential (ΔΨm in NPC-TW 076 cells. Results indicate that TET induced cell death through the cell morphological changes, caused G0/G1 phase arrest, increased ROS and Ca2+ production, and finally caused apoptotic cell death in NPC-TW 076 cells. There was no influence on the level of ΔΨm after TET treatment. Western blotting indicated that TET increased endoplasmic reticulum (ER stress associated protein expression such as GADD153, GRP78, ATF-6α and ATF-6 βwhich indicated that TET induced cell death through ER stress. ER stress is a potential target in cancer treatment, so the ability of TET to induce ER stress response and to activate programming cell death in NPC-TW 076 cells make this molecule become a promising anticancer agent.

  17. Tetrandrine Induces Apoptosis of Human Nasopharyngeal Carcinoma NPC-TW 076 Cells through Reactive Oxygen Species Accompanied by an Endoplasmic Reticulum Stress Signaling Pathway.

    Science.gov (United States)

    Lin, Ya-Jing; Peng, Shu-Fen; Lin, Meng-Liang; Kuo, Chao-Lin; Lu, Kung-Wen; Liao, Ching-Lung; Ma, Yi-Shih; Chueh, Fu-Shin; Liu, Kuo-Ching; Yu, Fu-Shun; Chung, Jing-Gung

    2016-10-12

    Nasopharyngeal carcinoma (NPC) is an epithelial malignancy of the head and neck and the incidence is higher in Southeast Asia. Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid, a natural product, and exhibits biological activities including action against many human cancer cell lines. However, the molecular mechanism of TET-induced cell apoptosis in human NPC cells is still unclear. In the present study, we investigated TET-induced apoptotic cell death and associated possible signal pathways on human nasopharyngeal carcinoma NPC-TW 076 cells in vitro. Phase contrast microscopy was used to examine cell morphology and DAPI staining was used to examine chromatin condensation. Flow cytometry assay was used to measure total viable cells, cell cycle and sub-G₁ phase distribution, reactive oxygen species (ROS), Ca(2+), and mitochondria membrane potential (ΔΨm) in NPC-TW 076 cells. Results indicate that TET induced cell death through the cell morphological changes, caused G₀/G₁ phase arrest, increased ROS and Ca(2+) production, and finally caused apoptotic cell death in NPC-TW 076 cells. There was no influence on the level of ΔΨm after TET treatment. Western blotting indicated that TET increased endoplasmic reticulum (ER) stress associated protein expression such as GADD153, GRP78, ATF-6α and ATF-6 βwhich indicated that TET induced cell death through ER stress. ER stress is a potential target in cancer treatment, so the ability of TET to induce ER stress response and to activate programming cell death in NPC-TW 076 cells make this molecule become a promising anticancer agent.

  18. C-cell hyperplasia accompanying thyroid diseases other than medullary carcinoma: an immunocytochemical study by means of antibodies to calcitonin and somatostatin.

    Science.gov (United States)

    Scopsi, L; Di Palma, S; Ferrari, C; Holst, J J; Rehfeld, J F; Rilke, F

    1991-05-01

    Eighteen normal thyroid glands and unaffected thyroid tissue adjacent to 37 follicular cell-derived benign and malignant tumors and to ten thyroid metastases were studied immunocytochemically with calcitonin (CT) and prosomatostatin/somatostatin (SMS) antibodies. CT- and SMS-immunoreactive cells were found in 100% of cases, though with ample variations in number. Most but not all SMS-immunoreactive cells also contained CT. Diffuse and/or nodular C-cell hyperplasia was seen in 30% of pathological thyroid glands; in concomitance with follicular adenomas, the mean C-cell number more than doubled that found in normal glands. Furthermore the proportion of SMS-immunoreactive C-cells increased from about 1% of CT-immunoreactive cells in normal adult thyroid glands to 2.5% in follicular adenomas, 3% in follicular carcinomas, 4.6% in papillary carcinomas, and 5.7% in metastases. The findings suggest that C-cell hyperplasia may be causally related to pathologic disorders affecting follicular cells. Furthermore, the demonstration that the intrathyroidal SMS cell mass is readily affected by alterations of the follicular structure of the gland suggests a possible regulatory role of SMS in the thyroidal microenvironment.

  19. Age-related decrease in the proportion of germinal center B cells from mouse Peyer's patches is accompanied by an accumulation of somatic mutations in their immunoglobulin genes.

    Science.gov (United States)

    González-Fernández, A; Gilmore, D; Milstein, C

    1994-11-01

    Somatic hypermutation of immunoglobulin genes and the generation of memory B cells seems to take place in germinal centers, which are chronically present in Peyer's patches. Age-associated changes in the germinal center B cell compartment of Peyer's patches and in the mutations of a kappa light chain transgene were analyzed in unimmunized mice. Somatic mutations accumulate in germinal center B cells slowly and continuously to reach an apparent plateau when the animals are around 5 months old. In contrast, the proportion of germinal center B cells reaches a maximum in very young mice (about 2 months old) and decreases progressively thereafter. These results suggest that the highly mutated B cells in older mice arise by the successive accumulation of mutations in memory cells. The data also show that the optimum time for the analysis of hypermutation of transgenes in Peyer's patches is when the mice are about 5 months old.

  20. Impact of cycling at low temperatures on the safety behavior of 18650-type lithium ion cells: Combined study of mechanical and thermal abuse testing accompanied by post-mortem analysis

    Science.gov (United States)

    Friesen, Alex; Horsthemke, Fabian; Mönnighoff, Xaver; Brunklaus, Gunther; Krafft, Roman; Börner, Markus; Risthaus, Tim; Winter, Martin; Schappacher, Falko M.

    2016-12-01

    The impact of cycling at low temperatures on the thermal and mechanical abuse behavior of commercial 18650-type lithium ion cells was compared to fresh cells. Post-mortem analyses revealed a deposition of high surface area lithium (HSAL) metal on the graphite surface accompanied by severe electrolyte decomposition. Heat wait search (HWS) tests in an accelerating rate calorimeter (ARC) were performed to investigate the thermal abuse behavior of aged and fresh cells under quasi-adiabatic conditions, showing a strong shift of the onset temperature for exothermic reactions. HSAL deposition promotes the reduction of the carbonate based electrolyte due to the high reactivity of lithium metal with high surface area, leading to a thermally induced decomposition of the electrolyte to produce volatile gaseous products. Nail penetration tests showed a change in the thermal runaway (TR) behavior affected by the decomposition reaction. This study indicates a greater thermal hazard for LIB cells at higher SOC and experiencing aging at low temperature.

  1. [Ischemic Changes in the Electrocardiogram and Circulatory Collapse Accompanied by Severe Anemia Owing to the Delay of Red Blood Cell Concentrate Transfusion in Two Patients with Intraoperative Massive Bleeding].

    Science.gov (United States)

    Horiuchi, Toshinori; Noguchi, Teruo; Kurita, Naoko; Yamaguchi, Ayako; Takeda, Masafumi; Sha, Keiichi; Nagahata, Toshihiro

    2016-01-01

    We present two patients developing intraoperative massive bleeding and showed ischemic changes in the electrocardiogram and circulatory collapse accompanied by severe anemia owing to the delay of red blood cell concentrate transfusion. One patient underwent hepatectomy and the other pancreaticoduodenectomy. Their lowest hemoglobin concentration was around 2 g x dl(-1), and they showed ischemic changes in the electrocardiogram and severe decreases in blood pressure. The former received compatible red blood cell concentrate and the latter received uncrossmatched same blood group red blood cell concentrate immediately, and their electrocardiogram and blood pressure quickly improved. To avoid life-threatening anemia, emergency red blood cell concentrate transfusion including compatible different blood group transfusion should be applied for intraoperative massive bleeding.

  2. Pathogen-induced proapoptotic phenotype and high CD95 (Fas expression accompany a suboptimal CD8+ T-cell response: reversal by adenoviral vaccine.

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    José Ronnie Vasconcelos

    Full Text Available MHC class Ia-restricted CD8(+ T cells are important mediators of the adaptive immune response against infections caused by intracellular microorganisms. Whereas antigen-specific effector CD8(+ T cells can clear infection caused by intracellular pathogens, in some circumstances, the immune response is suboptimal and the microorganisms survive, causing host death or chronic infection. Here, we explored the cellular and molecular mechanisms that could explain why CD8(+ T cell-mediated immunity during infection with the human protozoan parasite Trypanosoma cruzi is not optimal. For that purpose, we compared the CD8(+ T-cell mediated immune responses in mice infected with T. cruzi or vaccinated with a recombinant adenovirus expressing an immunodominant parasite antigen. Several functional and phenotypic characteristics of specific CD8(+ T cells overlapped. Among few exceptions was an accelerated expansion of the immune response in adenoviral vaccinated mice when compared to infected ones. Also, there was an upregulated expression of the apoptotic-signaling receptor CD95 on the surface of specific T cells from infected mice, which was not observed in the case of adenoviral-vaccinated mice. Most importantly, adenoviral vaccine provided at the time of infection significantly reduced the upregulation of CD95 expression and the proapoptotic phenotype of pathogen-specific CD8(+ cells expanded during infection. In parallel, infected adenovirus-vaccinated mice had a stronger CD8 T-cell mediated immune response and survived an otherwise lethal infection. We concluded that a suboptimal CD8(+ T-cell response is associated with an upregulation of CD95 expression and a proapoptotic phenotype. Both can be blocked by adenoviral vaccination.

  3. 18 SrRNA Degradation is Not Accompanied by Altered rRNA Transport at Early Times Following Irradiation of HeLa Cells

    Science.gov (United States)

    1990-01-01

    Altered rRNA Transport at Early Times following Irradiation of HeLa Cells PINHAS Ft CHS.* JOHN M. KROLAKt DAVID MCCLAINt AND KENNETH W. MINTONt "I...ratio of 2:1 that is ob- high as 1.6:1 during the interval 5 to 20 h following irradiation of HeLa cells at _7.5 Gy. Alterations in 28 S:18 S ratio were...m 2 5 15 20 GY findings that the movement of the newly formed 40 S ribo- 10 somal subunits from the nuclei in HeLa cells proceeds more 2swiftly

  4. Increased levels of soluble CD226 in sera accompanied by decreased membrane CD226 expression on peripheral blood mononuclear cells from cancer patients

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    Xu Zhuwei

    2009-06-01

    Full Text Available Abstract Background As a cellular membrane triggering receptor, CD226 is involved in the NK cell- or CTL-mediated lysis of tumor cells of different origin, including freshly isolated tumor cells and tumor cell lines. Here, we evaluated soluble CD226 (sCD226 levels in sera, and membrane CD226 (mCD226 expression on peripheral blood mononuclear cells (PBMC from cancer patients as well as normal subjects, and demonstrated the possible function and origin of the altered sCD226, which may provide useful information for understanding the mechanisms of tumor escape and for immunodiagnosis and immunotherapy. Results Soluble CD226 levels in serum samples from cancer patients were significantly higher than those in healthy individuals (P P Conclusion These findings suggest that sCD226 might be shed from cell membranes by certain proteases, and, further, sCD226 may be used as a predictor for monitoring cancer, and more important, a possible immunotherapy target, which may be useful in clinical application.

  5. Apoptosis induction in human breast cancer (MCF-7) cells by a novel venom L-amino acid oxidase (Rusvinoxidase) is independent of its enzymatic activity and is accompanied by caspase-7 activation and reactive oxygen species production.

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    Mukherjee, Ashis K; Saviola, Anthony J; Burns, Patrick D; Mackessy, Stephen P

    2015-10-01

    We report the elucidation of a mechanism of apoptosis induction in breast cancer (MCF-7) cells by an L-amino acid oxidase (LAAO), Rusvinoxidase, purified from the venom of Daboia russelii russelii. Peptide mass fingerprinting analysis of Rusvinoxidase, an acidic monomeric glycoprotein with a mass of ~57 kDa, confirmed its identity as snake venom LAAO. The enzymatic activity of Rusvinoxidase was completely abolished after two cycles of freezing and thawing; however, its cytotoxicity toward MCF-7 cells remained unaffected. Dose- and time-dependent induction of apoptosis by Rusvinoxidase on MCF-7 cells was evident from changes in cell morphology, cell membrane integrity, shrinkage of cells and apoptotic body formation accompanied by DNA fragmentation. Rusvinoxidase induced apoptosis in MCF-7 cells by both the extrinsic (death-receptor) and intrinsic (mitochondrial) signaling pathways. The former pathway of apoptosis operated through activation of caspase-8 that subsequently activated caspase-7 but not caspase-3. Rusvinoxidase-induced intrinsic pathway of apoptosis was accompanied by a time-dependent depolarization of the mitochondrial membrane through the generation of reactive oxygen species, followed by a decrease in cellular glutathione content and catalase activity, and down-regulation of expression of anti-apoptotic proteins Bcl-XL and heat-shock proteins (HSP-90 and HSP-70). Rusvinoxidase treatment resulted in increase of the pro-apoptotic protein Bax, subsequently leading to the release of cytochrome c from mitochondria to the cytosol and activating caspase-9, which in turn stimulated effector caspase-7. Rusvinoxidase at a dose of 4 mg/kg was non-toxic in mice, indicating that it may be useful as a model for the development of peptide-based anticancer drugs.

  6. PE11 (Rv1169c Selectively Altering Fatty Acid Components of Mycobacterium smegmatis and Host interleukin-6 Level Accompany with Cell Death

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    Wanyan eDeng

    2015-06-01

    Full Text Available PE/PPE family proteins, named after their conserved PE (Pro-Glu and PPE (Pro-Pro-Glu domains of N-terminal, are most intriguing aspects of pathologic mycobacterial genome. The roles of most members of this family remain unknown, although selected genes of this family are related to the virulence of Mycobacterium tuberculosis. In order to decipher the role of Rv1169c, the Mycobacterium smegmatis strain heterologously expressed this ORF was constructed and identified that Rv1169c was a cell wall associated protein with a novel function in modifying the cell wall fatty acid component.. The growth of Rv1169c expressing strain was affected under surface stress, acidic condition and antibiotics norfloxacin and rifampicin treatment. M. smegmatis expressing Rv1169c induced necrotic cell death of macrophage after infection and significantly decreased interlukin-6 production compared to controls. In general, these results underscore a proposing role of Rv1169c in virulence of M. tuberculosis, as it’s role in the susceptibility of anti-mycobacteria factors caused by modified cell wall fatty acid, and the induced necrotic cell death by Rv1169c is crucial for M. tuberculosis virulence during infection.

  7. Exposure of JB-6 mouse epidermal cells to 12-O-tetradecanoyl-phorbol-13-acetate is not accompanied by a significant change in total DNA-cytosine methylation.

    Science.gov (United States)

    Bondy, G P; Denhardt, D T

    1983-12-01

    The extent of methylation of the cytosine bases in DNA is believed to be a major factor influencing gene expression in eukaryotic cells. We have asked whether the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) alters the amount of 5-methylcytosine in DNA. The amount and relative distribution of 5-methylcytosine in the DNA of two subclones of the JB-6 mouse epidermal cell line were determined respectively by high performance liquid chromatography and digestion with the restriction enzymes MspI and HpaII. Exposure to TPA for up to several cell generations had no detectable effect on the degree of DNA methylation (3.9% of the total cytosine) in the two JB-6 lines or Friend erythroleukemia cells. Reduced methylation was readily detected in DNA extracted from cells exposed to 5-azacytidine. The data suggest that tumor promotion (at least that induced by TPA) is likely not the consequence of a generalized elevation or reduction in the amount of 5-methyl-cytosine in the DNA.

  8. Hypothyroidism Induces a Moderate Steatohepatitis Accompanied by Liver Regeneration, Mast Cells Infiltration, and Changes in the Expression of the Farnesoid X Receptor.

    Science.gov (United States)

    Rodríguez-Castelán, J; Corona-Pérez, A; Nicolás-Toledo, L; Martínez-Gómez, M; Castelán, F; Cuevas-Romero, E

    2017-03-01

    Hypothyroidism is associated with the development of non-alcoholic steatohepatitis, but cellular mechanisms have been scarcely analyzed. Thyroid hormones regulate the synthesis and secretion of bile acids that are endogenous ligands of the farnesoid receptor (FXRα), which have been involved in the development of non-alcoholic steatohepatitis. However, the relationship between thyroid hormones and FXRα expression in the liver is yet unknown. Control (n=6) and methimazole-induced hypothyroid (n=6) female rabbits were used to evaluate the amount of lipids and glycogen, vascularization, hepatocytes proliferation, immune cells infiltration, and expression of FXRα. Student-t or Mann-Whitney U tests were carried out to determine significant differences. Hypothyroidism induced steatosis, glycogen loss, fibrosis, and a minor vascularization in the liver. In contrast, hypothyroidism increased the proliferation of hepatocytes and the infiltration of mast cells, but did not modify the number of immune cells into sinusoids. These changes were associated with a minor anti-FXRα immunoreactivity of periportal hepatocytes and pericentral immune cells. Our results suggest that hypothyroidism induces a moderate non-alcoholic steatohepatitis, alllowing the hepatic regeneration. The FXRα may be involved in the development of non-alcoholic steatohepatitis in hypothyroid subjects.

  9. Grape seed extract induces apoptotic death of human prostate carcinoma DU145 cells via caspases activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release.

    Science.gov (United States)

    Agarwal, Chapla; Singh, Rana P; Agarwal, Rajesh

    2002-11-01

    Grape seed extract (GSE), rich in the bioflavonoids commonly known as procyanidins, is one of the most commonly consumed dietary supplements in the United States because of its several health benefits. Epidemiological studies show that many prostate cancer (PCA) patients use herbal extracts as dietary supplements in addition to their prescription drugs. Accordingly, in recent years, we have focused our attention on assessing the efficacy of GSE against PCA. Our studies showed that GSE inhibits growth and induces apoptotic death of human PCA cells in culture and in nude mice. Here, we performed detailed studies to define the molecular mechanism of GSE-induced apoptosis in advanced human PCA DU145 cells. GSE treatment of cells at various doses (50-200 micro g/ml) for 12-72 h resulted in a moderate to strong apoptotic death in a dose- and time-dependent manner. In the studies assessing the apoptotic-signaling pathway induced by GSE, we observed an increase in cleaved fragments of caspases 3, 7 and 9 as well as PARP in GSE-treated cells after 48 and 72 h of treatment. Pre-treatment of cells with general caspases inhibitor, z-Val-Ala-Asp(OMe)-FMK or caspase 3-like proteases inhibitor [z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK], almost completely (approximately 90%) inhibited the GSE-induced apoptotic cell death. In a later case, GSE-induced caspase-3 activity was completely inhibited. Selective caspase 9 inhibitor [z-Leu-Glu(OMe)-His-Asp(OMe)-FMK] showed only partial inhibition of GSE-induced apoptosis whereas GSE-induced protease activity of caspase 9 was completely inhibited. Upstream of caspase cascade, GSE showed disappearance of mitochondrial membrane potential and an increase in cytochrome c release in cytosol. Together, these results suggest that GSE possibly causes mitochondrial damage leading to cytochrome c release in cytosol and activation of caspases resulting in PARP cleavage and execution of apoptotic death of human PCA DU145 cells. Furthermore, GSE

  10. High-dose survival in the lymphocytic choriomeningitis virus infection is accompanied by suppressed DTH but unaffected T-cell cytotoxicity

    DEFF Research Database (Denmark)

    Marker, O; Thomsen, Allan Randrup; Volkert, M

    1985-01-01

    cells and infected sensitive targets in the central nervous system. On the other hand, high doses of virus caused a clear suppression of the LCMV-specific delayed-type hypersensitivity (DTH). In addition, when splenocytes from high-dose animals were transferred either intravenously or locally...... in mice infected with these doses of virus. In the high-dose mice we found generally higher organ virus titres and serum interferon titres than in the low-dose mice. Since we could demonstrate that virus-specific T-cell cytotoxicity in spleen, peripheral blood, and meningeal exudate was similar after...... intracerebral infection with large and small virus doses, and since the LCMV infection in the brain qualitatively and quantitatively was independent of the size of virus inoculum, the explanation for the survival of the high-dose animals is obviously not lack of possibilities for interaction between cytotoxic T...

  11. Acute NMDA toxicity in cultured rat cerebellar granule neurons is accompanied by autophagy induction and late onset autophagic cell death phenotype

    Directory of Open Access Journals (Sweden)

    Kobeissy Firas H

    2010-02-01

    Full Text Available Abstract Background Autophagy, an intracellular response to stress, is characterized by double membrane cytosolic vesicles called autophagosomes. Prolonged autophagy is known to result in autophagic (Type II cell death. This study examined the potential role of an autophagic response in cultured cerebellar granule neurons challenged with excitotoxin N-methyl-D-aspartate (NMDA. Results NMDA exposure induced light chain-3 (LC-3-immunopositive and monodansylcadaverine (MDC fluorescent dye-labeled autophagosome formation in both cell bodies and neurites as early as 3 hours post-treatment. Elevated levels of Beclin-1 and the autophagosome-targeting LC3-II were also observed following NMDA exposure. Prolonged exposure of the cultures to NMDA (8-24 h generated MDC-, LC3-positive autophagosomal bodies, concomitant with the neurodegenerative phase of NMDA challenge. Lysosomal inhibition studies also suggest that NMDA-treatment diverted the autophagosome-associated LC3-II from the normal lysosomal degradation pathway. Autophagy inhibitor 3-methyladenine significantly reduced NMDA-induced LC3-II/LC3-I ratio increase, accumulation of autophagosomes, and suppressed NMDA-mediated neuronal death. ATG7 siRNA studies also showed neuroprotective effects following NMDA treatment. Conclusions Collectively, this study shows that autophagy machinery is robustly induced in cultured neurons subjected to prolonged exposure to excitotoxin, while autophagosome clearance by lysosomal pathway might be impaired. Our data further show that prolonged autophagy contributes to cell death in NMDA-mediated excitotoxicity.

  12. 18S rRNA degradation is not accompanied by altered rRNA transport at early times following irradiation of HeLa cells

    Energy Technology Data Exchange (ETDEWEB)

    Fuchs, P.; Krolak, J.M.; McClain, D.; Minton, K.W.

    1990-01-01

    In recent investigations on the effects of radiation on rRNA processing in HeLa S3 cells, the authors pulse-labeled the cells with uridine immediately prior to irradiation. The 45 S rRNA precursor, which undergoes nuclear processing to form one each of its major daughter species, 28S and 18S rRNA, was separated from the daughter species by gel electrophoresis and the radiolabel in each species determined at various times after irradiation. By pulse-labeling the cells prior to irradiation, superimposed effects caused by radiation-induced alterations of rRNA transcription and Refs. therein were minimized, permitting selective analysis of the processing of that fraction of 45S precursor that had been synthesized (radiolabeled) predominantly prior to irradiation. They now report more detailed studies on 45S rRNA processing within the first 2 h following irradiation in which they have found a maximum 28 S:18 S ratio of 2:1 that is observed about 1 h following irradiation of 5 or 10 Gy.

  13. [Acquired angioedema with C1-INH deficiency and accompanying chronic spontaneous urticaria in a patient with chronic lymphatic B cell leukemia].

    Science.gov (United States)

    Klossowski, N; Braun, S A; von Gruben, V; Losem, C; Plewe, D; Homey, B; Meller, S

    2015-10-01

    Acquired angioedema due to C1 inhibitor deficiency (C1-INH-AAE) is characterized by recurrent edema of the subcutaneous and/or submucosal tissue without wheals and negative family history of angioedema. Here, we present the case of a patient with a chronic lymphatic B cell leukemia who suffered from both C1-INH-AAE and chronic spontaneous urticaria. Oral corticosteroids, antihistamines, and the anti-IgE antibody omalizumab were applied to treat the chronic urticaria in combination with the plasma-derived C1 esterase inhibitor concentrate Berinert® and the bradykinin B2 receptor antagonist icatibant, but the symptoms did not improved significantly. Thus, polychemotherapy targeting the slow-growing lymphoproliferative disease including rituximab was initiated, which resulted in remission of both the urticaria and the angioedema.

  14. Third cranial nerve palsy (ptosis, diplopia accompanied by orbital swelling: case report of unusual clinical presentation of giant cell arteritis associated with polymyalgia rheumatica

    Directory of Open Access Journals (Sweden)

    Prassede Bravi

    2012-12-01

    Full Text Available IntroductionGiant cell arteritis (GCA is the most common systemic vasculitis in older individuals, characterized by granulomatosus inflammation of the wall of large and medium-sized arteries. The wide spectrum of arterial sites involved leads to ischemia of different organs resulting in a wide range of clinical signs and symptoms. Temporal artery is commonly involved (temporal arteritis. Unusual patterns of presentation, such as extraocular motility disorders and orbital swelling, may be early and transient manifestations of GCA and precede the permanent visual loss due to ischemic optic neuropathy.Case reportWe describe a patient with uncommon manifestations of GCA consisting of transient recurrent diplopia, ptosis, orbital swelling together with more typical clinical features of the disease such as musculoskeletal manifestations (polymyalgia rheumatica and facial pain: all signs and symptoms promptly resolved under corticosteroid therapy without relapse.Conclusions A high level of suspicion of GCA in individuals over the age of 50 years is needed to prevent the development of severe complications. Clinicians should be aware of uncommon manifestations of the disease such as head–neck swelling and ophthalmoplegia: management guidelines have stated that prompt administration of adequate dose of corticosteroids as soon as ocular manifestations of GCA are noted may almost totally prevent blindness.

  15. Release of Apical Dominance in Potato Tuber Is Accompanied by Programmed Cell Death in the Apical Bud Meristem[C][W

    Science.gov (United States)

    Teper-Bamnolker, Paula; Buskila, Yossi; Lopesco, Yael; Ben-Dor, Shifra; Saad, Inbal; Holdengreber, Vered; Belausov, Eduard; Zemach, Hanita; Ori, Naomi; Lers, Amnon; Eshel, Dani

    2012-01-01

    Potato (Solanum tuberosum) tuber, a swollen underground stem, is used as a model system for the study of dormancy release and sprouting. Natural dormancy release, at room temperature, is initiated by tuber apical bud meristem (TAB-meristem) sprouting characterized by apical dominance (AD). Dormancy is shortened by treatments such as bromoethane (BE), which mimics the phenotype of dormancy release in cold storage by inducing early sprouting of several buds simultaneously. We studied the mechanisms governing TAB-meristem dominance release. TAB-meristem decapitation resulted in the development of increasing numbers of axillary buds with time in storage, suggesting the need for autonomous dormancy release of each bud prior to control by the apical bud. Hallmarks of programmed cell death (PCD) were identified in the TAB-meristems during normal growth, and these were more extensive when AD was lost following either extended cold storage or BE treatment. Hallmarks included DNA fragmentation, induced gene expression of vacuolar processing enzyme1 (VPE1), and elevated VPE activity. VPE1 protein was semipurified from BE-treated apical buds, and its endogenous activity was fully inhibited by a cysteinyl aspartate-specific protease-1-specific inhibitor N-Acetyl-Tyr-Val-Ala-Asp-CHO (Ac-YVAD-CHO). Transmission electron microscopy further revealed PCD-related structural alterations in the TAB-meristem of BE-treated tubers: a knob-like body in the vacuole, development of cytoplasmic vesicles, and budding-like nuclear segmentations. Treatment of tubers with BE and then VPE inhibitor induced faster growth and recovered AD in detached and nondetached apical buds, respectively. We hypothesize that PCD occurrence is associated with the weakening of tuber AD, allowing early sprouting of mature lateral buds. PMID:22362870

  16. Antiproliferative potential of a novel parthenin analog P16 as evident by apoptosis accompanied by down-regulation of PI3K/AKT and ERK pathways in human acute lymphoblastic leukemia MOLT-4 cells.

    Science.gov (United States)

    Goswami, Akshra; Shah, Bhahwal Ali; Kumar, Ajay; Rizvi, Masood Ahmad; Kumar, Suresh; Bhushan, Shashi; Malik, Fayaz Ahmed; Batra, Navneet; Joshi, Amit; Singh, Jagtar

    2014-10-05

    Leukemia is one of the deadliest types of cancer. Lack of effective treatment strategies has resulted in an extensive quest for new therapeutic molecules against it. This study explores the molecular mechanism of anticancer activity of P16, a semisynthetic analog of parthenin, against the human acute lymphoblastic leukemia MOLT-4 cells. P16 displayed antiproliferative activity in different cancer cell lines; however, MOLT-4 cells showed highest sensitivity for P16 with IC50 value of 0.6μM. Further studies revealed that P16 induced cell death by apoptosis. It caused mitochondrial stress, which was mediated by the translocation of Bax from cytosol to mitochondria and release of cytochrome c into the cytosol and consequent activation of caspase-9. However, P16 was also able to activate caspase-8, thus involving both extrinsic and intrinsic pathways of apoptosis. Further, activation of caspase-3 led to cleavage of its target proteins PARP-1 and ICAD, which resulted in apoptotic DNA damage. P16 induced apoptosis was accompanied by the down-regulation of important leukemic cell survival proteins like pAKT (S473), pAKT (T308), pP70S6K, pCRAF, and pERK1/2. However, inhibition of caspases by Z-VAD-FMK reversed the down-regulatory effect of P16 on pAKT (S473) and pP70S6K, as evident by the cell viability assay and flow cytometric analysis but this inhibition did not completely reverse the antiproliferative effect of P16, thereby indicating the role of additional factors apart from caspases in P16 induced apoptosis in MOLT-4 cells. Owing to its antiproliferative potential against leukemia cells, P16 can further be explored as an effective therapeutics against leukemia. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  17. Neutronless -accompanied ternary fission of

    Science.gov (United States)

    Sandulescu, A.; Cârstoiu, F.; Misicu, S.; Florescu, A.; Ramayya, A. V.; Hamilton, J. H.; Greiner, W.

    1998-01-01

    A new type of decay corresponding to the neutronless -accompanied fragmentation of is studied. We employ a cluster model similar to the model used for the description of cluster radioactivity. No preformation factors were considered. The ternary relative isotopic yields were calculated as the ratio of the penetrability of a given ternary fragmentation over the sum of penetrabilities of all possible ternary neutronless fragmentations. The corresponding barriers between the light and heavy fragments and between the cluster and the two heavier fragments were computed with the help of a double-folding potential generated by M3Y-NN effective interaction and realistic fragment ground-state deformations. Also, we studied the influence of the fragment excitation energies on the yields, by including the level densities and the -stretching of the fragments. The new phenomenon could be experimentally observed by the triple-gamma coincidence technique between the fragments and .

  18. [Wernicke encephalopathy accompanying linitis plastica].

    Science.gov (United States)

    Soós, Zsuzsanna; Salamon, Mónika; Oláh, Roland; Czégeni, Anna; Salamon, Ferenc; Folyovich, András; Winkler, Gábor

    2014-01-05

    Wernicke encephalopathy (or Wernicke-Korsakoff encephalopathy) is a rarely diagnosed neurological disorder, which is caused by vitamin B1 deficiency. In the classical form it is characterized by a typical triad (confusion, oculomotor disturbance and ataxia), however, in the majority of the cases only confusion is present. It can be frequently observed in subjects with chronic alcohol consumption, but it may accompany different pathological states of which end stage malignant diseases are the most importants, where confusion may have different backgrounds. The authors present the case of an old male patient with advanced gastric cancer recognised and treated vitamin B1 deficiency, and they draw attention to difficulties of the diagnosis of Wernicke's disease.

  19. "Wilms Tumor Protein 1" (WT1) peptide vaccination-induced complete remission in a patient with acute myeloid leukemia is accompanied by the emergence of a predominant T-cell clone both in blood and bone marrow.

    Science.gov (United States)

    Ochsenreither, Sebastian; Fusi, Alberto; Busse, Antonia; Bauer, Sandra; Scheibenbogen, Carmen; Stather, David; Thiel, Eckhard; Keilholz, Ulrich; Letsch, Anne

    2011-01-01

    Within the last few years, the first peptide vaccination trials for treatment of acute myeloid leukemia (AML) have been initiated. Athough the presence of epitope-specific T cells could be seen both in bone marrow (BM) and peripheral blood (PB), nothing is known about their clonal composition. In this study, we analyzed material from a patient with recurrent AML vaccinated with "Wilms Tumor Protein 1" (WT1) peptide, who achieved a complete remission (CR) lasting for 12 months. For identification of expanded WT1-specific T-cell clones, enrichment by tetramer and IFNγ secretion were followed by comparative quantitative reverse transcribed PCR (qRT PCR) quantification of all TCR Vβ-families. Vβ-families with increase in the enriched fraction were cloned and sequenced. A predominant clone was quantified by clonotypic qRT PCR from PB and BM. Quantity and functionality of WT1-specific cells were assessed by tetramer analyses and intracellular IFNγ staining. A specific predominant clone was identified during clinical remission. Clone-specific qRT PCR showed an increase both in PB and BM after 8 vaccinations. Six months after achieving CR, the transcript levels in BM decreased. Relapse was accompanied by secondary rise of the WT1-specific clone in PB but not in BM. In parallel, a lack of vaccine-induced WT1 specific IFNγ production was observed at that timepoint. In conclusion, we provide first data regarding evolution and compartmentalization of a peptide vaccine-induced T-cell clone in PB and BM of an AML patient. At the time of relapse, the same clone reappeared spontaneously in PB but not in BM showing impaired functionality.

  20. 19 CFR 148.4 - Accompanying articles.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Accompanying articles. 148.4 Section 148.4 Customs... (CONTINUED) PERSONAL DECLARATIONS AND EXEMPTIONS General Provisions § 148.4 Accompanying articles. (a) Generally. Articles shall be considered as accompanying a passenger or brought in by him if the...

  1. Adult-Onset Acquired Partial Lipodystrophy Accompanied by Rheumatoid Arthritis

    Directory of Open Access Journals (Sweden)

    Yusuke Muto

    2015-04-01

    Full Text Available Lipodystrophy is a group of metabolic disorders, possibly caused by autoimmune disease. In this report, we describe a case of adult-onset acquired partial lipodystrophy accompanied by rheumatoid arthritis without a family history. Interestingly, immunohistochemical staining revealed dense infiltration of IL-27-producing cells as well as MMP-7- and MMP-28-expressing cells, both of which have been reported to facilitate the development of autoimmune disease. Our present case might suggest possible mechanisms for acquired partial lipodystrophy.

  2. Creating and Editing Video to Accompany Manuscripts.

    Science.gov (United States)

    Gordon, Shayna L; Porto, Dennis A; Ozog, David M; Council, M Laurin

    2016-02-01

    The use of video can enhance the learning experience by demonstrating procedural techniques that are difficult to relay in writing. Several peer-reviewed journals allow publication of videos alongside articles to complement the written text. The purpose of this article is to instruct the dermatologic surgeon on how to create and edit a video using a smartphone, to accompany a article. The authors describe simple tips to optimize surgical videography. The video that accompanies this article further demonstrates the techniques described. Creating a surgical video requires little experience or equipment and can be completed in a modest amount of time. Making and editing a video to accompany a article can be accomplished by following the simple recommendations in this article. In addition, the increased use of video in dermatologic surgery education can enhance the learning opportunity.

  3. Novice Collaboration in Solo and Accompaniment Improvisation

    DEFF Research Database (Denmark)

    Hansen, Anne-Marie; Andersen, Hans Jørgen

    2012-01-01

    This study investigates how non-musicians engaged in a solo-accompaniment music improvisation relationship. Seven user teams interacted with two electronic music instruments integrated in two pen tablets. One instrument was a melody instrument and the other a chord instru-ment. The study was done...

  4. Khz (fusion product of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in human colon carcinoma HCT116 cells, accompanied by an increase in reactive oxygen species, activation of caspase 3, and increased intracellular Ca²⁺.

    Science.gov (United States)

    Kim, Tae Hwan; Kim, Ju Sung; Kim, Zoo Haye; Huang, Ren Bin; Chae, Young Lye; Wang, Ren Sheng

    2015-03-01

    Khz (a fusion mycelium of Ganoderma lucidum and Polyporus umbellatus mycelia) is isolated from ganoderic acid and P. umbellatus and it exerts antiproliferative effects against malignant cells. However, no previous study has reported the inhibitory effects of Khz on the growth of human colon cancer cells. In the present study, we found that Khz suppressed cell division and induced apoptosis in HCT116 cells. Khz cytotoxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Khz reduced cell viability and mitochondrial membrane potential levels and it also induced disruption of the mitochondrial membrane potential and increased calcium concentration and reactive oxygen species generation. Khz increased caspase 3, PARP, caspase 7, and caspase 9 levels, but reduced Bcl-2 protein levels. Flow cytometry showed that the percentage of HCT116 cells in the sub-G1 phase of the cell cycle increased in response to Khz treatment.

  5. Solutions manual to accompany Fundamentals of calculus

    CERN Document Server

    Morris, Carla C

    2015-01-01

    Solutions Manual to Accompany Fundamentals of Calculus the text that encourages students to use power, quotient, and product rules for solutions as well as stresses the importance of modeling skills.  In addition to core integral and differential calculus coverage, the core book features finite calculus, which lends itself to modeling and spreadsheets.  Specifically, finite calculus is applied to marginal economic analysis, finance, growth, and decay.  Includes: Linear Equations and Functions The Derivative Using the Derivative Exponential and Logarithmic

  6. Severe Hypoglycemia Accompanied with Thyroid Crisis

    Directory of Open Access Journals (Sweden)

    Yuki Nakatani

    2012-01-01

    Full Text Available We report a 32-year-old Japanese women with severe hypoglycemia accompanied with thyroid crisis. She complained of dyspnea, general fatigue, and leg edema. She was diagnosed with hyperthyroidism with congestive heart failure and liver dysfunction. Soon after admission, sudden cardiopulmonary arrest occurred. She was then transferred to the intensive care unit. Her serum glucose level was 7 mg/dl. Intravenous glucose, hydrocortisone, diuretics, and continuous hemodiafiltration (CHDF saved her. We considered that hypoglycemia occurred due to heart failure and liver dysfunction due to thyroid crisis.

  7. Solutions manual to accompany Nonlinear programming

    CERN Document Server

    Bazaraa, Mokhtar S; Shetty, C M

    2014-01-01

    As the Solutions Manual, this book is meant to accompany the main title, Nonlinear Programming: Theory and Algorithms, Third Edition. This book presents recent developments of key topics in nonlinear programming (NLP) using a logical and self-contained format. The volume is divided into three sections: convex analysis, optimality conditions, and dual computational techniques. Precise statements of algortihms are given along with convergence analysis. Each chapter contains detailed numerical examples, graphical illustrations, and numerous exercises to aid readers in understanding the concepts a

  8. The ACOSTA accompanying measure A2106.

    Science.gov (United States)

    Thayer, C; De Moor, G; Van Goor, J

    1994-10-01

    At a time when the informatics and telecommunications industries are looking for new markets to exploit in relation, for example, to the emerging ISDN and broadband communications networks, there is a need to create a broad consensus in Europe by bringing together systematically the relevant industries including telecom service providers, health care providers, insurance organisations, standardisation experts and policy makers. The aim of the ACOSTA (Consensus Formation and Standardisation Promotion) Accompanying Measure is the creation of more general awareness of the relevant environment among all the parties, better specification of common requirements and options taking better account of the real needs of the users, and enlargement of the common market in health care telematics.

  9. Bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis

    Directory of Open Access Journals (Sweden)

    Nilufer Kocak

    2014-01-01

    Full Text Available X-linked juvenile retinoschisis is a rare hereditary retinal disease characterized by a tangential splitting of the neurosensory retina which may cause early-onset visual impairment. Existence of the retinal neurosensory layer splitting on cross-sectional images of optical coherance tomography (OCT and the absence of leakage on fluorescein angiography (FA help confirming the diagnosis. Such diagnostic tests are also helpful in determining the management of the disease. However, most of the retinoschisis cavities remain stable and rarely extend to the posterior pole, many authors suggest laser prophylaxis to avoid the potential risk of retinal detachment due to holes in the outer retinal layer. Herein, we report a case with bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis who was evaluated with detailed ophthalmologic examination. Visual acuity, fundoscopy, OCT, and FA remained stable in the second year of follow-up after prophylactic argon laser treatment.

  10. Tumefactive Brain Demyelination Accompanying MADSAM Neuropathy

    Directory of Open Access Journals (Sweden)

    Şefik Evren Erdener

    2015-09-01

    Full Text Available Multifocal acquired demyelinating sensory and motor (MADSAM neuropathy is characterized by asymmetric multifocal motor and sensory loss and conduction blocks in peripheral nerves. Peripheral demyelinating diseases may be accompanied by demyelination in central nervous system (CNS. In this report, a MADSAM patient with a solitary tumefactive demyelinating lesion in brain is presented. Neuroimaging due to a visual field defect revealed a right parietooccipital lesion, which was initially misdiagnosed as a tumor. Pathological examination showed that it was demyelinating in nature. Peripheral nervous symptoms of the patient developed two years later and she was then diagnosed with MADSAM. There was prominent clinical and electrophysiological response to steroid treatment. Tumefactive brain involvement was not previously reported for MADSAM neuropathy, although it was documented in a single case with typical chronic inflammatory demyelinating polyneuropathy (CIDP. CNS involvement should therefore be considered in MADSAM patients.

  11. RPF101, a new capsaicin-like analogue, disrupts the microtubule network accompanied by arrest in the G2/M phase, inducing apoptosis and mitotic catastrophe in the MCF-7 breast cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Sá-Júnior, Paulo Luiz de [Laboratory of Genetics, Butantan Institute, Vital Brasil Avenue 1500, Postal Code: 05503-900, Sao Paulo (Brazil); Pasqualoto, Kerly Fernanda Mesquita [Biochemistry and Biophysical Laboratory, Butantan Institute, Vital Brasil Avenue 1500, Postal Code: 05503-900, Sao Paulo (Brazil); Ferreira, Adilson Kleber [Laboratory of Genetics, Butantan Institute, Vital Brasil Avenue 1500, Postal Code: 05503-900, Sao Paulo (Brazil); Tavares, Maurício Temotheo; Damião, Mariana Celestina Frojuello Costa Bernstorff [Department of Pharmacy, School of Pharmaceutical Sciences, University of Sao Paulo, Prof. Lineu Prestes Avenue, 580, Postal Code: 05508-000, Sao Paulo (Brazil); Azevedo, Ricardo Alexandre de [Biochemistry and Biophysical Laboratory, Butantan Institute, Vital Brasil Avenue 1500, Postal Code: 05503-900, Sao Paulo (Brazil); Câmara, Diana Aparecida Dias; Pereira, Alexandre; Madeiro de Souza, Dener [Laboratory of Genetics, Butantan Institute, Vital Brasil Avenue 1500, Postal Code: 05503-900, Sao Paulo (Brazil); Parise Filho, Roberto, E-mail: roberto.parise@usp.br [Department of Pharmacy, School of Pharmaceutical Sciences, University of Sao Paulo, Prof. Lineu Prestes Avenue, 580, Postal Code: 05508-000, Sao Paulo (Brazil)

    2013-02-01

    Breast cancer is the world's leading cause of death among women. This situation imposes an urgent development of more selective and less toxic agents. The use of natural molecular fingerprints as sources for new bioactive chemical entities has proven to be a quite promising and efficient method. Capsaicin, which is the primary pungent compound in red peppers, was reported to selectively inhibit the growth of a variety tumor cell lines. Here, we report for the first time a novel synthetic capsaicin-like analogue, RPF101, which presents a high antitumor activity on MCF-7 cell line, inducing arrest of the cell cycle at the G2/M phase through a disruption of the microtubule network. Furthermore, it causes cellular morphologic changes characteristic of apoptosis and a decrease of Δψm. Molecular modeling studies corroborated the biological findings and suggested that RPF101, besides being a more reactive molecule towards its target, may also present a better pharmacokinetic profile than capsaicin. All these findings support the fact that RPF101 is a promising anticancer agent. -- Highlights: ► We report for the first time that RPF101 possesses anticancer properties. ► RPF101 induces apoptosis of human breast cancer cells. ► RPF 101 decreases mitochondrial potential and induces DNA fragmentation.

  12. Change of the equivalent circuit constants accompanied by the degradation and recovery of efficiency on a-Si solar cells; A-Si taiyo denchi no koritsu no rekka to kaifuku ni tomonau toka kairo teisu no henka

    Energy Technology Data Exchange (ETDEWEB)

    Takahisa, K.; Kojima, T.; Nakamura, K.; Koyanagi, T.; Yanagisawa, T. [Electrotechnical Laboratory, Tsukuba (Japan)

    1997-11-25

    Investigations were given on how the equivalent circuit constants change when efficiency of amorphous silicon solar cells changes with time in light degradation and temperature recovery. In the experiment, light irradiation tests under a constant temperature and light intensity condition, followed by recovery tests under a constant temperature and constant weak light intensity or constant temperature condition were repeated continuously. According to the result of an experiment on single layer type cells, the change in each equivalent circuit constant in association with degradation in efficiency and file factor and variation in recovery is reversible mostly. However, a slightly irreversible component was recognized only in the initial degradation process in series resistance and diode factor values. With regard to stacked cells, it was suggested that the main players to determine cell characteristics during the processes of deterioration and recovery take turns among the three layers as follows: the shape of the time-based change in the efficiency comes different and is not saturating; as the efficiency decreases, the extent of the change increases in the diode factor and series resistance; and the path the deterioration takes differs from that the recovery takes. 2 refs., 12 figs.

  13. Trimethyltin-evoked apoptosis of murine hippocampal granule neurons is accompanied by the expression of interleukin-1beta and interleukin-1 receptor antagonist in cells of ameboid phenotype, the majority of which are NG2-positive.

    Science.gov (United States)

    Fiedorowicz, Anna; Figiel, Izabela; Zaremba, Małgorzata; Dzwonek, Karolina; Schliebs, Reinhard; Oderfeld-Nowak, Barbara

    2008-09-05

    Interleukin-1beta (IL-1beta) has been implicated in various neuropathologies, while IL-1 receptor antagonist (IL-1ra) has been shown to reduce neuronal injury. We investigated the pattern of expression of both cytokines in murine hippocampus after trimethyltin (TMT) intoxication. Using a ribonuclease protection assay, we demonstrated induction of transcription of IL-1beta and IL-1ra 3 days following TMT treatment which correlated with the peak of neuronal apoptosis. At this time, immunocytochemical staining revealed enhanced expression of both cytokines in NG2 proteoglycan expressing ameboid cells located at the site of neurotoxic insult, some of which bound also the microglial marker, lectin. There was some overlap between NG2 and lectin staining. Our results suggest that the two cytokines are involved in apoptotic processes in dentate granule cells and indicate that the pro-apoptotic effect of IL-1beta prevails over the presumed protective action of IL-1ra. The novel finding of expression of both cytokines in NG2(+) cells of ameboid phenotype indicates that these cells, through the regulatory roles of pro- and anti-inflammatory cytokines, may be involved in control of neuronal death or survival after injury.

  14. Acquisition of docetaxel resistance in breast cancer cells reveals upregulation of ABCB1 expression as a key mediator of resistance accompanied by discrete upregulation of other specific genes and pathways

    DEFF Research Database (Denmark)

    Ninel Hansen, Stine; Westergaard, David; Borg Houlberg Thomsen, Mathilde

    2015-01-01

    to be prominent at higher docetaxel concentrations (second-phase response). Additional resistance mechanisms were indicated by gene expression profiling, including genes in the interferon-inducible protein family in MCF7RES and cancer testis antigen family in MDARES. Also, upregulated expression of various ABC...... resistance and thereby identify key molecular mechanisms and predictive molecular characteristics to docetaxel resistance. Two docetaxel-resistant cell lines, MCF7RES and MDARES, were generated from their respective parental cell lines MCF-7 and MDA-MB-231 by stepwise selection in docetaxel dose increments...... analysis singled out ABCB1, which encodes permeability glycoprotein (Pgp), as the top upregulated gene in both MCF7RES and MDARES. Functional validation revealed Pgp as a key resistance mediator at low docetaxel concentrations (first-phase response), whereas additional resistance mechanisms appeared...

  15. Production of interleukin (IL)-5 and IL-10 accompanies T helper cell type 1 (Th1) cytokine responses to a major thyroid self-antigen, thyroglobulin, in health and autoimmune thyroid disease

    DEFF Research Database (Denmark)

    Nielsen, C H; Hegedüs, L; Rieneck, K;

    2007-01-01

    Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma exert detrimental effects in organ-specific autoimmune disease, while both destructive and protective roles have been demonstrated for interleukin (IL)-10, IL-4 and IL-5. We examined the production of these cytokines by peripheral bloo......-gamma, IL-5 and IL-10 responses were markedly inhibited by partial denaturation of Tg by boiling. We hypothesize that autoantibodies and complement may promote mixed Th1/Th2 cell cytokine responses by enhancing the uptake of autoantigens by antigen-presenting cells....... appeared to promote the production of IL-2 and particularly IL-5, the levels of which were reduced by neutralization of complement by heat- or zymosan treatment. The production of IFN-gamma and IL-2 of the three groups together correlated directly with the serum anti-Tg activity. Moreover, TNF-alpha, IFN...

  16. Production of interleukin (IL)-5 and IL-10 accompanies T helper cell type 1 (Th1) cytokine responses to a major thyroid self-antigen, thyroglobulin, in health and autoimmune thyroid disease

    DEFF Research Database (Denmark)

    Nielsen, Claus H; Hegedüs, L; Rieneck, Klaus

    2007-01-01

    Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma exert detrimental effects in organ-specific autoimmune disease, while both destructive and protective roles have been demonstrated for interleukin (IL)-10, IL-4 and IL-5. We examined the production of these cytokines by peripheral bloo......-gamma, IL-5 and IL-10 responses were markedly inhibited by partial denaturation of Tg by boiling. We hypothesize that autoantibodies and complement may promote mixed Th1/Th2 cell cytokine responses by enhancing the uptake of autoantigens by antigen-presenting cells...... appeared to promote the production of IL-2 and particularly IL-5, the levels of which were reduced by neutralization of complement by heat- or zymosan treatment. The production of IFN-gamma and IL-2 of the three groups together correlated directly with the serum anti-Tg activity. Moreover, TNF-alpha, IFN...

  17. Expression pattern of transcription factors and intracellular cytokines reveals that clinically cured tuberculosis is accompanied by an increase in Mycobacterium-specific Th1, Th2, and Th17 cells.

    Science.gov (United States)

    da Silva, Marcos V; Massaro Junior, Vladimir J; Machado, Juliana R; Silva, Djalma A A; Castellano, Lúcio R; Alexandre, Patricia B D; Rodrigues, Denise B R; Rodrigues, Virmondes

    2015-01-01

    Tuberculosis (TB) remains a major global health problem and is the second biggest cause of death by infectious disease worldwide. Here, we investigate in vitro the Th1, Th2, Th17, and Treg cytokines and transcriptional factors produced after Mycobacterium-specific antigen stimulation in patients with active pulmonary tuberculosis, clinically cured pulmonary tuberculosis, and healthy donors with a positive tuberculin skin test (TST+). Together, our data indicate that clinical cure after treatment increases the percentages of Mycobacterium-specific Th1, Th2, and Th17 cells compared with those found in active-TB and TST+ healthy donors. These results show that the host-parasite equilibrium in latent TB breaks in favor of the microorganism and that the subsequent clinical recovery posttreatment does not return the percentage levels of such cells to those observed in latent tuberculosis. Additionally, our results indicate that rather than showing an increase in the percentage of Mycobacterium-specific Tregs, active-TB patients display lower Th1 : Treg and Th17 : Treg ratios. These data, together with lower Th1 : Th2 and Th17 : Th2 ratios, may indicate a mechanism by which the breakdown of the host-parasite equilibrium leads to active-TB and changes in the repertoire of Mycobacterium-specific Th cells that are associated with clinical cure after treatment of pulmonary tuberculosis.

  18. Expression Pattern of Transcription Factors and Intracellular Cytokines Reveals That Clinically Cured Tuberculosis Is Accompanied by an Increase in Mycobacterium-Specific Th1, Th2, and Th17 Cells

    Directory of Open Access Journals (Sweden)

    Marcos V. da Silva

    2015-01-01

    Full Text Available Tuberculosis (TB remains a major global health problem and is the second biggest cause of death by infectious disease worldwide. Here, we investigate in vitro the Th1, Th2, Th17, and Treg cytokines and transcriptional factors produced after Mycobacterium-specific antigen stimulation in patients with active pulmonary tuberculosis, clinically cured pulmonary tuberculosis, and healthy donors with a positive tuberculin skin test (TST+. Together, our data indicate that clinical cure after treatment increases the percentages of Mycobacterium-specific Th1, Th2, and Th17 cells compared with those found in active-TB and TST+ healthy donors. These results show that the host-parasite equilibrium in latent TB breaks in favor of the microorganism and that the subsequent clinical recovery posttreatment does not return the percentage levels of such cells to those observed in latent tuberculosis. Additionally, our results indicate that rather than showing an increase in the percentage of Mycobacterium-specific Tregs, active-TB patients display lower Th1 : Treg and Th17 : Treg ratios. These data, together with lower Th1 : Th2 and Th17 : Th2 ratios, may indicate a mechanism by which the breakdown of the host-parasite equilibrium leads to active-TB and changes in the repertoire of Mycobacterium-specific Th cells that are associated with clinical cure after treatment of pulmonary tuberculosis.

  19. Anaplastic thyroid carcinoma accompanied by uncontrollable eosinophilia.

    Science.gov (United States)

    Shiraishi, Jun; Koyama, Hidenori; Seki, Miwako; Hatayama, Miki; Naka, Mariko; Kurajoh, Masafumi; Okazaki, Hirokazu; Shoji, Takuhito; Moriwaki, Yuji; Yamamoto, Tetsuya; Tsuchida, Yasuaki; Tsukamoto, Yoshitane; Hirota, Seiichi; Onoda, Naoyoshi; Namba, Mitsuyoshi

    2015-01-01

    Anaplastic thyroid carcinoma is a rare disease, and cases associated with eosinophilia are even rarer. We herein report a case of anaplastic thyroid carcinoma accompanied by remarkable and uncontrollable eosinophilia. A 71-year-old man was diagnosed with end-stage anaplastic thyroid carcinoma. Throughout the aggressive clinical course of the cancer, eosinophilia dramatically progressed and became extremely refractory to steroid treatment. We measured the serum levels of hematopoietic cytokines potentially involved in eosinophilia, including granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. Although the GM-CSF level was moderately elevated, both the IL-3 and IL-5 levels were within the normal ranges. In this case, the patient's eosinophilia may have been related to his severe dyspnea and was likely responsible for the allergic reaction to the anticancer drug. Therefore, it is essential to elucidate the etiology of eosinophilia in patients with thyroid cancer in order to improve the treatment for patients with anaplastic thyroid carcinoma.

  20. Hemimegalencephaly accompanied by myoclonic status epilepticus.

    Science.gov (United States)

    Nakashima, Kentarou; Ogihara, Masaaki; Kasai-Yoshida, Emi; Tsuruta, Shio; Nakagawa, Machiko; Kusakawa, Isao

    2012-07-01

    We describe a boy (aged 2 years and 7 months) with hemimegalencephaly who developed myoclonic status, which improved dramatically after total callosotomy. The patient experienced seizures beginning at age 2 days, at which time electroencephalography revealed a right unilateral burst suppression pattern, and cranial magnetic resonance imaging revealed an enlarged right hemisphere. At age 8 months, habitual seizures increased to more than daily frequency. At the same time, myoclonic status epilepticus appeared with frequent erratic, partial, massive myoclonic seizures and clouding of consciousness. These signs were accompanied by diffuse spike and spike-wave patterns on electroencephalography, indicating myoclonic status in nonprogressive encephalopathy. Total callosotomy performed at age 10 months resulted in the complete disappearance of myoclonic status and prominent decrease in habitual seizures. This description of hemimegalencephaly is the first, to our knowledge, in which total callosotomy alleviated myoclonic status epilepticus. Although the mechanism of myoclonic status epilepticus remains unknown, our results suggest that cortico-cortical pathways are involved in this type of myoclonic status. Copyright © 2012 Elsevier Inc. All rights reserved.

  1. Review: Regional land subsidence accompanying groundwater extraction

    Science.gov (United States)

    Galloway, Devin L.; Burbey, Thomas J.

    2011-01-01

    The extraction of groundwater can generate land subsidence by causing the compaction of susceptible aquifer systems, typically unconsolidated alluvial or basin-fill aquifer systems comprising aquifers and aquitards. Various ground-based and remotely sensed methods are used to measure and map subsidence. Many areas of subsidence caused by groundwater pumping have been identified and monitored, and corrective measures to slow or halt subsidence have been devised. Two principal means are used to mitigate subsidence caused by groundwater withdrawal—reduction of groundwater withdrawal, and artificial recharge. Analysis and simulation of aquifer-system compaction follow from the basic relations between head, stress, compressibility, and groundwater flow and are addressed primarily using two approaches—one based on conventional groundwater flow theory and one based on linear poroelasticity theory. Research and development to improve the assessment and analysis of aquifer-system compaction, the accompanying subsidence and potential ground ruptures are needed in the topic areas of the hydromechanical behavior of aquitards, the role of horizontal deformation, the application of differential synthetic aperture radar interferometry, and the regional-scale simulation of coupled groundwater flow and aquifer-system deformation to support resource management and hazard mitigation measures.

  2. Changes of the temperature coefficients of the characteristics which accompany degradation and recovery of a-Si solar cells; A-Si taiyo denchi no hikari (denryu) rekka oyobi kaifuku ni tomonau tokusei ondo keisu no henka

    Energy Technology Data Exchange (ETDEWEB)

    Yanagisawa, T.; Koyanagi, T.; Nakamura, K.; Takahisa, K.; Kojima, T. [electrotechnical Laboratory, Tsukuba (Japan)

    1996-10-27

    Pursuant to the measuring of temperature dependency of the characteristics such as conversion efficiency, during the process of degradation in a-Si solar cells due to light and electric current and the process of recovery by annealing, this paper describes changes in temperature coefficients, correlation between the characteristic parameters and the degradation, and the results of the examination of their characteristics. The conversion efficiency {mu} degraded approximately by 45% of the initial value each by the irradiation under a light intensity with 3 SUN accelerated and by the infusion of current at 20mA/cm{sup 2}; and then, the efficiency recovered to 70-75% of the degradation by subsequent annealing. In addition, in the temperature dependency at 80{degree}C against at 20{degree}C, Isc slightly increased while Vcc greatly decreased. This slight increase in Isc was mainly due to the decrease in the width of the forbidden band, while the decrease in Vcc was due to the increase in the reverse saturation current. The temperature dependency of {mu}N was negative, becoming small in accordance with the degradation. The temperature dependency of FF/FFO was negative initially both in light and current, but it decreased with the degradation and turned to positive. The temperature coefficients of I-V parameters reversibly changed corresponding to the degradation and recovery of these parameters and stayed in a good correlation. 7 refs., 8 figs., 1 tab.

  3. Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation.

    Directory of Open Access Journals (Sweden)

    Yui Terayama

    Full Text Available Hyperplastic candidiasis is characterized by thickening of the mucosal epithelia with Candida albicans infection with occasional progression to squamous cell carcinoma (SCC. C. albicans is a critical factor in tumor development; however, the oncogenic mechanism is unclear. We have previously produced an animal model for hyperplastic candidiasis in the rat forestomach. In the present study, we investigate whether impaired DNA methylation and associated protein expression of tumor suppressor and DNA repair genes are involved in the SCC carcinogenesis process using this hyperplastic candidiasis model. Promoter methylation and protein expression were analyzed by methylation specific PCR and immunohistochemical staining, respectively, of 5 areas in the forestomachs of alloxan-induced diabetic rats with hyperplastic candidiasis: normal squamous epithelia, squamous hyperplasia, squamous hyperplasia adjacent to SCC, squamous hyperplasia transitioning to SCC, and SCC. We observed nuclear p16 overexpression despite increases in p16 gene promoter methylation during the carcinogenic process. TIMP3 and RAR-β2 promoter methylation progressed until the precancerous stage but disappeared upon malignant transformation. In comparison, TIMP3 protein expression was suppressed during carcinogenesis and RAR-β2 expression was attenuated in the cytoplasm but enhanced in nuclei. ERCC1 and BRCA1 promoters were not methylated at any stage; however, their protein expression disappeared beginning at hyperplasia and nuclear protein re-expression in SCC was observed only for ERCC1. These results suggest that aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 expression might occur that is inconsistent with the respective gene promoter methylation status, and that this overexpression might serve to promote the inflammatory carcinogenesis caused by C. albicans infection.

  4. Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation.

    Science.gov (United States)

    Terayama, Yui; Matsuura, Tetsuro; Ozaki, Kiyokazu

    2016-01-01

    Hyperplastic candidiasis is characterized by thickening of the mucosal epithelia with Candida albicans infection with occasional progression to squamous cell carcinoma (SCC). C. albicans is a critical factor in tumor development; however, the oncogenic mechanism is unclear. We have previously produced an animal model for hyperplastic candidiasis in the rat forestomach. In the present study, we investigate whether impaired DNA methylation and associated protein expression of tumor suppressor and DNA repair genes are involved in the SCC carcinogenesis process using this hyperplastic candidiasis model. Promoter methylation and protein expression were analyzed by methylation specific PCR and immunohistochemical staining, respectively, of 5 areas in the forestomachs of alloxan-induced diabetic rats with hyperplastic candidiasis: normal squamous epithelia, squamous hyperplasia, squamous hyperplasia adjacent to SCC, squamous hyperplasia transitioning to SCC, and SCC. We observed nuclear p16 overexpression despite increases in p16 gene promoter methylation during the carcinogenic process. TIMP3 and RAR-β2 promoter methylation progressed until the precancerous stage but disappeared upon malignant transformation. In comparison, TIMP3 protein expression was suppressed during carcinogenesis and RAR-β2 expression was attenuated in the cytoplasm but enhanced in nuclei. ERCC1 and BRCA1 promoters were not methylated at any stage; however, their protein expression disappeared beginning at hyperplasia and nuclear protein re-expression in SCC was observed only for ERCC1. These results suggest that aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 expression might occur that is inconsistent with the respective gene promoter methylation status, and that this overexpression might serve to promote the inflammatory carcinogenesis caused by C. albicans infection.

  5. Hiperplasia de células neuroendócrinas pulmonares difusas com obstrução ao fluxo aéreo Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia accompanied by airflow obstruction

    Directory of Open Access Journals (Sweden)

    Ester Nei Aparecida Martins Coletta

    2009-05-01

    Full Text Available A hiperplasia de células neuroendócrinas pulmonares difusas com obstrução ao fluxo aéreo é uma lesão pulmonar rara. Todos os casos publicados foram diagnosticados por biópsia pulmonar cirúrgica. Apenas três casos relatados apresentavam opacidades intersticiais difusas na TCAR. Nós relatamos três casos adicionais desta entidade. Todos eram mulheres, com obstrução leve ou moderada ao fluxo aéreo. No primeiro caso, uma biópsia transbrônquica, associada com dados de imagem foram considerados suficientes para o diagnóstico. Um padrão em mosaico foi observado nos três casos, mas no terceiro um infiltrado pulmonar difuso foi também observado. Em casos muito raros, o aspecto na TCAR pode simular aquele encontrado em outras doenças pulmonares intersticiais.Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia with airflow obstruction is a rare form of lung injury. All of the reported cases have been diagnosed by surgical lung biopsy. Only three of the reported cases presented with diffuse interstitial lung opacities on HRCT scans. We report three additional cases of this entity. All of the patients were female and presented with mild-to-moderate airflow obstruction. In the first case, transbronchial biopsy and imaging data were sufficient to make the diagnosis. Although the HRCT scans of all three cases revealed a mosaic pattern, that of the third patient also revealed diffuse interstitial infiltrate. In extremely rare cases, HRCT findings can simulate those seen in other interstitial lung diseases.

  6. 16 CFR 1500.125 - Labeling requirements for accompanying literature.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Labeling requirements for accompanying literature. 1500.125 Section 1500.125 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL... REGULATIONS § 1500.125 Labeling requirements for accompanying literature. When any accompanying...

  7. Relationship between Obstructive Sleep Apnea Syndrome Accompanied by Hypertension and Red Blood Cell Distribution Width%阻塞性睡眠呼吸暂停综合征合并高血压与红细胞分布宽度的关系

    Institute of Scientific and Technical Information of China (English)

    徐劲松; 程亚慧; 夏国际

    2014-01-01

    目的:探讨阻塞性睡眠呼吸暂停综合征(OSAS)合并高血压与红细胞分布宽度(RDW)的关系。方法选择2011年1月至2013年8月在中国人民解放军第九四医院呼吸内科门诊及住院的 OSAS 合并高血压患者(OSAS合并高血压组)44例和体检中心体检的原发性高血压患者(高血压组)57例、健康体检者(正常对照组)52例。各组清晨空腹抽取肘静脉血2 mL,用全自动五分类血细胞分析仪检测红细胞(RBC)计数、血红蛋白(Hb)、RDW 及白细胞(WBC)计数,比较各组 RDW 的差异,并进行相关性分析。结果高血压组 RBC 计数、Hb、RDW 和 WBC 计数与正常对照组比较差异均无统计学意义(均 P >0.05);OSAS 合并高血压组 BMI、DBP、RBC 计数、Hb 和 RDW 均明显高于正常对照组、高血压组,SBP 明显高于正常对照组、明显低于高血压组(均 P <0.05);高血压组 SBP、DBP均明显高于正常对照组(均 P <0.05)。相关性分析结果显示,OSAS 合并高血压组 RDW 与睡眠呼吸暂停低通气指数(AHI)呈正相关(r=0.426,P <0.05),与最低氧饱和度呈负相关(r=-0.509,P <0.05)。结论高血压患者RDW 无明显改变,RDW 增高可能是 OSAS 合并高血压患者心脑血管事件的标志物。%Objective To explore the relationship between obstructive sleep apnea syndrome (OSAS)accompanied by hypertension and red blood cell distribution width(RDW).Methods Forty-four patients with OSAS accompanied by hypertension who were treated in the department of respiratory medicine in the 94th Hospital of the Chinese People’s Liberation Army from January 2011 to August 2013(OSAS accompanied by hypertension group)and 57 patients with primary hypertension(hypertension group)and 52 healthy physical examinees(normal control group)who were examined at physical examination center during the same period were selected in this

  8. The accompanying adult: authority to give consent in the UK.

    Science.gov (United States)

    Lal, Seema Madhur Lata; Parekh, Susan; Mason, Carol; Roberts, Graham

    2007-05-01

    Children may be accompanied by various people when attending for dental treatment. Before treatment is started, there is a legal requirement that the operator obtain informed consent for the proposed procedure. In the case of minors, the person authorized to give consent (parental responsibility) is usually a parent. To ascertain if accompanying persons of children attending the Department of Paediatric Dentistry at the Eastman Dental Hospital, London were empowered to give consent for the child's dental treatment. A total of 250 accompanying persons of children attending were selected, over a 6-month period. A questionnaire was used to establish whether the accompanying person(s) were authorized to give consent. The study showed that 12% of accompanying persons had no legal authority to give consent for the child's dental treatment. Clinicians need to be aware of the status of persons accompanying children to ensure valid consent is obtained.

  9. 9 CFR 93.307 - Articles accompanying horses.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying horses. 93.307... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses § 93.307 Articles accompanying horses. No..., blankets, or other things used for or about horses governed by the regulations this part, shall be...

  10. Harmonizing Melody with Meta-Structure of Piano Accompaniment Figure

    Institute of Scientific and Technical Information of China (English)

    Yin Feng; Kui Chen; Xiang-Bin Liu

    2011-01-01

    In this paper,a meta-structure of piano accompaniment figure (meta-structure for short) is proposed to harmonize a melodic piece of music so as to construct a multi-voice music.Here we approach melody harmonization with piano accompaniment as a machine learning task in a probabilistic framework.A series of piano accompaniment figures are collected from the massive existing sample scores and converted into a set of meta-structure.After the procedure of samples training,a model is formulated to generate a proper piano accompaniment figure for a harmonizing unit in the context.This model is flexible in harmonizing a melody with piano accompaniment.The experimental results are evaluated and discussed.

  11. Herpes zoster laryngitis accompanied by Ramsay Hunt syndrome.

    Science.gov (United States)

    Lee, Dong Hoon; Yoon, Tae Mi; Lee, Joon Kyoo; Joo, Young Eun; Lim, Sang Chul

    2013-01-01

    The most common presentation of herpes zoster in the head and neck region is called Ramsay Hunt syndrome (RHS), which rarely accompanies multiple cranial neuropathy. Herpes zoster also involves the mucous membrane of the tongue, palate, pharynx, and larynx. Herpes zoster infection of the larynx accompanied by Ramsay Hunt syndrome with cranial polyneuropathy is extremely rare, with only few reported cases in the literature. At the time of this report, a review of the medical literature disclosed 4 reported cases of herpes zoster laryngitis accompanied by Ramsay Hunt syndrome. Herein, we present 2 additional cases and report the clinical outcome of cranial polyneuropathy with a review of the literature.

  12. Correlation between thyroid function and nodular goiter accompanied with gallstone

    Institute of Scientific and Technical Information of China (English)

    Shihong Ma; Qinjiang Liu; Xiaofeng Hou

    2014-01-01

    The-purpose-of-the-study-wass-to-explore-the-correlation-between-thyroid-function-and-nodular-goiter-accompanied-with-gal-stone.-Methods:We-col-ected-120-cases-about-nodular-goiter-accompanied-with-gal-stone-and-128-cases-about-nodular-goiter-and-establish-50-healthy-control-groups.-Detected-t-level-of-hyrotropic-hormone-(TSH),-total-tri-o-dothyronine-(TT3),-total-thyroxine-in-the-peripheral-venous-blood-of-these-cases-in-the-three-groups-by-using-electrochemilu-minescence-immunoassay,-measure-level-of-total-cholesterol-(TC),-high-density-lipoprotein-cholesterol-(HDL-C),-low-density-lipoprotein-cholesterol-(LDL-C)-and-total-bile-acid-(TBA)-levels-by-using-enzymic-method,-and-observed-the-changes-of-thyroid-function-and-blood-lipid-among-the-three-groups.-Results:The-serum-TT3-level-in-nodular-goiter-accompanied-with-gal-stone-group-and-the-nodular-goiter-group-was-significantly-lower-than-that-in-control-group-(P0.05).-Accordingly,-TC-and-LDL-C-level-in-nodular-goiter-accompanied-with-gal-stone-group-was-significantly-higher-than-that-in-nodular-goiter-and-control-group-(P0.05).-The-HDL-C-level-in-nodular-goiter-accompanied-with-gal-stone-group-and-control-group-was-higher-than-that-in-simple-nodular-goiter-group-(P<0.01).-Conclusion:The-originating-etiologic-factor-of-nodular-goiter-accompanied-with-gal-stone-may-be-related-to-that-the-decreased-TT3-induced-sub-clinical-hypothyroidism.

  13. Epo receptors are not detectable in primary human tumor tissue samples.

    Directory of Open Access Journals (Sweden)

    Steve Elliott

    Full Text Available Erythropoietin (Epo is a cytokine that binds and activates an Epo receptor (EpoR expressed on the surface of erythroid progenitor cells to promote erythropoiesis. While early studies suggested EpoR transcripts were expressed exclusively in the erythroid compartment, low-level EpoR transcripts were detected in nonhematopoietic tissues and tumor cell lines using sensitive RT-PCR methods. However due to the widespread use of nonspecific anti-EpoR antibodies there are conflicting data on EpoR protein expression. In tumor cell lines and normal human tissues examined with a specific and sensitive monoclonal antibody to human EpoR (A82, little/no EpoR protein was detected and it was not functional. In contrast, EpoR protein was reportedly detectable in a breast tumor cell line (MCF-7 and breast cancer tissues with an anti-EpoR polyclonal antibody (M-20, and functional responses to rHuEpo were reported with MCF-7 cells. In another study, a functional response was reported with the lung tumor cell line (NCI-H838 at physiological levels of rHuEpo. However, the specificity of M-20 is in question and the absence of appropriate negative controls raise questions about possible false-positive effects. Here we show that with A82, no EpoR protein was detectable in normal human and matching cancer tissues from breast, lung, colon, ovary and skin with little/no EpoR in MCF-7 and most other breast and lung tumor cell lines. We show further that M-20 provides false positive staining with tissues and it binds to a non-EpoR protein that migrates at the same size as EpoR with MCF-7 lysates. EpoR protein was detectable with NCI-H838 cells, but no rHuEpo-induced phosphorylation of AKT, STAT3, pS6RP or STAT5 was observed suggesting the EpoR was not functional. Taken together these results raise questions about the hypothesis that most tumors express high levels of functional EpoR protein.

  14. Anterior internal lenticonus accompanied by congenital nuclear cataract

    Institute of Scientific and Technical Information of China (English)

    LIU Zhe; SUN Chuan-bin; YAO Ke

    2011-01-01

    Internal lenticonus is a very rare morphologic abnormality of crystalline lens which has been reported in only several cases in the literature.We herein reported the clinical characteristics and surgical findings of the anterior internal lenticonus accompanied by congenital nuclear cataract.Cataract extraction accompanied with intraocular lens implantation was uneventfully performed,and a good visual outcome was achieved in this case.Viral infection during embryonal and fetal period might account for the formation of the anterior internal lenticonus and congenital nuclear cataract in our case.

  15. 29 CFR 575.5 - Supporting data to accompany application.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Supporting data to accompany application. 575.5 Section 575.5 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS WAIVER OF CHILD LABOR PROVISIONS FOR AGRICULTURAL EMPLOYMENT OF 10 AND 11 YEAR OLD MINORS IN HAND...

  16. The Global Education Practicum: Perspectives from Accompanying Academics

    Science.gov (United States)

    Lang, Catherine; Cacciattolo, Marcelle; Kidman, Gillian

    2017-01-01

    The benefits of international education experiences for students are well documented. The effect on the individual of international experiences has been researched and theorised by authors for at least the last 20 years. In this paper the experiences of three academics who accompanied pre-service teachers on a 3 week international practicum are…

  17. 9 CFR 93.208 - Articles accompanying poultry.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying poultry. 93.208 Section 93.208 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH, AND POULTRY,...

  18. Hypertrophic pachymeningitis accompanying neuromyelitis optica spectrum disorder: A case report.

    Science.gov (United States)

    Kon, Tomoya; Nishijima, Haruo; Haga, Rie; Funamizu, Yukihisa; Ueno, Tatsuya; Arai, Akira; Suzuki, Chieko; Nunomura, Jin-ichi; Baba, Masayuki; Takahashi, Toshiyuki; Tomiyama, Masahiko

    2015-10-15

    We report a case of idiopathic cerebral hypertrophic pachymeningitis accompanying neuromyelitis optica spectrum disorder. No other identifiable cause of pachymeningitis was detected. Corticosteroid therapy was effective for both diseases. Hypertrophic pachymeningitis is closely related to autoimmune inflammatory disease of the central nervous system. This case supports the hypothesis that hypertrophic pachymeningitis can be a rare comorbidity of neuromyelitis optica spectrum disorder.

  19. Critical thinking of student nurses during clinical accompaniment

    Directory of Open Access Journals (Sweden)

    BY Uys

    2005-09-01

    Full Text Available The purpose of this study was to investigate the methods of clinical accompaniment used by clinical facilitators in practice. The findings of the study also reflected facilitators’ perceptions regarding critical thinking and the facilitation thereof. A quantitative research design was used. A literature study was conducted to identify the methods of accompaniment that facilitate critical thinking. Data was collected by means of a questionnaire developed for that purpose. Making a content-related validity judgment, and involving seven clinical facilitators in an academic institution, ensured the validity of the questionnaire. The results of the study indicated that various clinical methods of accompaniment were used. To a large extent, these methods correlated with those discussed in the literature review. The researcher further concluded that the concepts ‘critical thinking’ and ‘facilitation’ were not interpreted correctly by the respondents, and would therefore not be implemented in a proper manner in nursing practice. Furthermore, it seemed evident that tutor-driven learning realised more often than student-driven learning. In this regard, the requirement of outcomes-based education was not satisfied. The researcher is therefore of the opinion that a practical programme for the development of critical thinking skills during clinical accompaniment must be developed within the framework of outcomes-based education.

  20. Accompanying Partners of International Students: Reflections on Three Issues

    Science.gov (United States)

    Cui, Dan; Arthur, Nancy; Domene, José F.

    2017-01-01

    This paper reviews and critiques the existing literature on accompanying partners of international students (APIS), who are often an ignored population in programs and services for the internationalization of Canadian higher education. Particularly, we identify three issues. First, we argue that current research on this group overwhelmingly…

  1. Teacher's Manual to Accompany "How to Get a Job."

    Science.gov (United States)

    Webster, John D.

    Prepared for teachers of mentally handicapped students, this teacher's guide accompanies the student booklet, "How to Get A Job," and contains suggestions for learning activities and vocabulary development for each subdivision of all four major units in the student booklet: choosing the right kind of work, looking for the job, going on an…

  2. Observation of T lymphocyte subsets in the liver of patients with advanced schistosomiasis and advanced schistosomiasis accompanied with hepatitis B.

    Science.gov (United States)

    Zhang, S; Li, Z; Cai, S; Zeng, L

    2000-01-01

    T lymphocyte subsets in the liver were detected by Avidin-Biotin Complex (ABC) assay in 22 patients with advanced schistosomiasis (AS) and 5 cases of AS accompanied with hepatitis B. T lymphocytes in the liver of AS patients were distributed in the peripheral layer of egg granuloma or the area near eggs in non-granuloma. No infiltrative T lymphocytes were observed in area with extensive fibrosis. There was infiltration of many T cells in the portal tract, piecemeal and focal necrosis area as well as in hepatic sinus in AS patients accompanied with hepatitis B. CD8+ T cells (suppressor/cytotoxic T cells, Ts/Tc) in the liver were predominant in the two groups. In AS patients, marked hepatic fibrosis, a small number of T cell infiltration and slight hepatocellular degeneration and necrosis were observed. However, obvious hepatocellular degeneration and necrosis were seen in AS patients accompanied with hepatitis B, and 3 cases of them developed active liver cirrhosis. The results indicated immune response was weak in the liver in AS patients and Ts cells might be predominant in the subset of CD8+ T lymphocytes. Cellular immune response was relatively strong in AS patients accompanied with hepatitis B and the infiltrative CD8+ T lymphocytes might be mainly Tc cells.

  3. Spectral distribution of solo voice and accompaniment in pop music.

    Science.gov (United States)

    Borch, Daniel Zangger; Sundberg, Johan

    2002-01-01

    Singers performing in popular styles of music mostly rely on feedback provided by monitor loudspeakers on the stage. The highest sound level that these loudspeakers can provide without feedback noise is often too low to be heard over the ambient sound level on the stage. Long-term-average spectra of some orchestral accompaniments typically used in pop music are compared with those of classical symphonic orchestras. In loud pop accompaniment the sound level difference between 0.5 and 2.5 kHz is similar to that of a Wagner orchestra. Long-term-average spectra of pop singers' voices showed no signs of a singer's formant but a peak near 3.5 kHz. It is suggested that pop singers' difficulties to hear their own voices may be reduced if the frequency range 3-4 kHz is boosted in the monitor sound.

  4. Bremsstrahlung emission of photons accompanying ternary fission of 252Cf

    Science.gov (United States)

    Maydanyuk, S. P.; Olkhovsky, V. S.; Mandaglio, G.; Manganaro, M.; Fazio, G.; Giardina, G.; Saccá, C.

    2011-02-01

    We present the first results on the bremsstrahlung emission of photons accompanying ternary spontaneous fission of the 252Cf nucleus. We also compare our calculations on the basis of quantum model with preliminary experimental data and find a good agreement between theory and experiment for photon energies up to 500 keV, when the α-particle emission is in presence of the field of two fission fragments of the daughter nucleus.

  5. The Raven,a Loner’s Psychological Accompanier

    Institute of Scientific and Technical Information of China (English)

    商滔

    2015-01-01

    Edgar Allan Poe’s The Raven is well-known to the world for its detailed psychological descriptions in the author’s eyes of this essay.Whereas,the Raven is the only one accompanie and the best listener listening to Poe’s psychological cry for his lover.The author will try to present the scene of the sadness from Poe by employing Carl G.Jung’s theories about consciousness and unconsciousness.

  6. The Raven,a Loner’s Psychological Accompanier

    Institute of Scientific and Technical Information of China (English)

    商滔

    2015-01-01

    Edgar Allan Poe’s The Raven is well-known to the world for its detailed psychological descriptions in the author’s eyes of this essay.Whereas,the Raven is the only one accompanier and the best listener listening to Poe’s psychological cry for his lover.The author will try to present the scene of the sadness from Poe by employing Carl G.Jung’s theories about consciousness and unconsciousness.

  7. Right Lung Agenesis; Isolated and with Accompanied Anomalies

    Directory of Open Access Journals (Sweden)

    Yakup Canıtez

    2013-12-01

    Full Text Available Right lung agenesis is a rare anomaly that can be isolated or accompanied by system anomalies such as cardiac, skeletal or urinary systems. Case 1, a four-month-old girl, was brought because of respiratory distress. Patient had polydactyly, syndactyly of right thumb, right mandibular hypoplasia and low-set dysmorphic ears. Lung x-ray and thorax computerized tomography (CT were consistent with right pulmonary agenesis and butterfly vertebra was evident in the 7th thoracic level. Thoracic CT angiography revealed narrowing of the left main bronchus and esophagus due to compression of aorta, left pulmonary artery and right atrium. In bronchoscopy, narrowing due to compression at the carina level was seen and right main bronchus was not seen. In abdominal ultrasonography, right kidney placement anomaly (pelvic ectopia was present and renal scintigraphy revealed fusion in both kidneys. With these findings, it was found that right pulmonary agenesis was accompanied by ipsilateral radial ray anomaly, renal anomaly, vertebral anomaly and hemifacial microsomia. Case 2, a fifteen-year-old male patient, was diagnosed as pulmonary agenesis via chest x-ray, pulmonary CT and bronchoscopy after a wheezing episode when he was 2 months old. Patient had no complaint except for exhaustion that is aggravated by exercise during last year. In physical examination, he had a mild scoliosis toward right, respiratory sounds were diminished on right hemithorax and heart sounds were heard on right side. Chest x-ray and thoracic CT were consistent with right lung agenesis. No abnormality was found in echocardiography and abdominal ultrasonography was normal. Accompanied cardiovascular anomalies, distortions of intrathoracic structures and recurrent infections are main factors that affect mortality and morbidity. Here, two cases with right lung agenesis, isolated and accompanied by multiple anomalies, were presented. (Jo­ur­nal of Cur­rent Pe­di­at­rics 2013; 11: 134-7

  8. Klinefelter's syndrome accompanied by diabetes mellitus and diabetes insipidus.

    Science.gov (United States)

    Isobe, K; Niwa, T; Ohkubo, M; Ohba, M; Shikano, M; Watanabe, Y

    1992-07-01

    The first case of Klinefelter's syndrome accompanied by diabetes insipidus and diabetes mellitus is reported. A 41-year-old man admitted for hyperosmolar diabetic coma with a past history of diabetes insipidus was diagnosed as having Klinefelter's syndrome by endocrinological examination and sex chromosome analysis. In this case, glucose tolerance test was normalized half a year later and blood glucose was well controlled with diet therapy alone.

  9. A case of peribiliary cysts accompanying bile duct carcinoma

    Institute of Scientific and Technical Information of China (English)

    Fumihiko Miura; Tadahiro Takada; Hodaka Amano; Masahiro Yoshida; Takahiro Isaka; Naoyuki Toyota; Keita Wada; Kenji Takagi; Kenichiro Karo

    2006-01-01

    A rare case of peribiliary cysts accompaying bile duct carcinoma is presented. A 54-year-old man was diagnosed as having lower bile duct carcinoma and peribiliary cysts by diagnostic imaging. He underwent pylorus preserving pancreatoduodenectomy. As for the peribiliary cysts, a course of observation was taken.Over surgery due to misdiagnosis of patients with biliary malignancy accompanied by peribiliary cysts should be avoided.

  10. The Stress and Vascular Catastrophes in Newborn Rats: Mechanisms Preceding and Accompanying the Brain Hemorrhages

    Science.gov (United States)

    Semyachkina-Glushkovskaya, Oxana; Borisova, Ekaterina; Abakumov, Maxim; Gorin, Dmitry; Avramov, Latchezar; Fedosov, Ivan; Namykin, Anton; Abdurashitov, Arkady; Serov, Alexander; Pavlov, Alexey; Zinchenko, Ekaterina; Lychagov, Vlad; Navolokin, Nikita; Shirokov, Alexander; Maslyakova, Galina; Zhu, Dan; Luo, Qingming; Chekhonin, Vladimir; Tuchin, Valery; Kurths, Jürgen

    2016-01-01

    In this study, we analyzed the time-depended scenario of stress response cascade preceding and accompanying brain hemorrhages in newborn rats using an interdisciplinary approach based on: a morphological analysis of brain tissues, coherent-domain optical technologies for visualization of the cerebral blood flow, monitoring of the cerebral oxygenation and the deformability of red blood cells (RBCs). Using a model of stress-induced brain hemorrhages (sound stress, 120 dB, 370 Hz), we studied changes in neonatal brain 2, 4, 6, 8 h after stress (the pre-hemorrhage, latent period) and 24 h after stress (the post-hemorrhage period). We found that latent period of brain hemorrhages is accompanied by gradual pathological changes in systemic, metabolic, and cellular levels of stress. The incidence of brain hemorrhages is characterized by a progression of these changes and the irreversible cell death in the brain areas involved in higher mental functions. These processes are realized via a time-depended reduction of cerebral venous blood flow and oxygenation that was accompanied by an increase in RBCs deformability. The significant depletion of the molecular layer of the prefrontal cortex and the pyramidal neurons, which are crucial for associative learning and attention, is developed as a consequence of homeostasis imbalance. Thus, stress-induced processes preceding and accompanying brain hemorrhages in neonatal period contribute to serious injuries of the brain blood circulation, cerebral metabolic activity and structural elements of cognitive function. These results are an informative platform for further studies of mechanisms underlying stress-induced brain hemorrhages during the first days of life that will improve the future generation's health. PMID:27378933

  11. Real-time implementation of an interactive jazz accompaniment system

    Science.gov (United States)

    Deshpande, Nikhil

    Modern computational algorithms and digital signal processing (DSP) are able to combine with human performers without forced or predetermined structure in order to create dynamic and real-time accompaniment systems. With modern computing power and intelligent algorithm layout and design, it is possible to achieve more detailed auditory analysis of live music. Using this information, computer code can follow and predict how a human's musical performance evolves, and use this to react in a musical manner. This project builds a real-time accompaniment system to perform together with live musicians, with a focus on live jazz performance and improvisation. The system utilizes a new polyphonic pitch detector and embeds it in an Ableton Live system - combined with Max for Live - to perform elements of audio analysis, generation, and triggering. The system also relies on tension curves and information rate calculations from the Creative Artificially Intuitive and Reasoning Agent (CAIRA) system to help understand and predict human improvisation. These metrics are vital to the core system and allow for extrapolated audio analysis. The system is able to react dynamically to a human performer, and can successfully accompany the human as an entire rhythm section.

  12. Test bank to accompany Computers data and processing

    CERN Document Server

    Deitel, Harvey M

    1980-01-01

    Test Bank to Accompany Computers and Data Processing provides a variety of questions from which instructors can easily custom tailor exams appropriate for their particular courses. This book contains over 4000 short-answer questions that span the full range of topics for introductory computing course.This book is organized into five parts encompassing 19 chapters. This text provides a very large number of questions so that instructors can produce different exam testing essentially the same topics in succeeding semesters. Three types of questions are included in this book, including multiple ch

  13. Study guide to accompany computers data and processing

    CERN Document Server

    Deitel, Harvey M

    1985-01-01

    Study Guide to Accompany Computer and Data Processing provides information pertinent to the fundamental aspects of computers and computer technology. This book presents the key benefits of using computers.Organized into five parts encompassing 19 chapters, this book begins with an overview of the evolution of modern computing systems from the earliest mechanical calculating devices to microchips. This text then introduces computer hardware and describes the processor. Other chapters describe how microprocessors are made and describe the physical operation of computers. This book discusses as w

  14. Bronchial Sparganosis mansoni accompanied by abnormal hyperplasia diagnosed by bronchoscopy

    Institute of Scientific and Technical Information of China (English)

    BAI Jing; HE Zhi-yi; LIU Guang-nan; ZHANG Jian-quan; DENG Jing-min; LI Mei-hua; ZHONG Xiao-ning

    2012-01-01

    Pulmonary sparganosis mansoni is rare in humans and bronchial sparganosis mansoni has not been reported.We reported a patient with a soft-tissue mass in the right hilum area on a chest computed tomography (CT) scan that was suspected of being lung cancer.Bronchoscopy identified sparganum larvae.Bronchial sparganosis mansoni accompanied by abnormal hyperplasia was diagnosed by histopathology.We introduced our experience and reviewed the clinical characteristics of three pulmonary sparganosis mansoni cases and three pleural cavity sparganosis mansoni cases that have been reoorted.

  15. Mucocele Accompanied by a Traumatic Neuroma: A Case Report

    Directory of Open Access Journals (Sweden)

    Jaafari Ashkavandi Z.

    2013-03-01

    Full Text Available Mucocele and traumatic neuroma are two lesions related to the traumatic events; however there is only one reported case in which these two entities were perceived simultaneously. The current study reported a 21-year-old man who complained of painless recurrent swelling, accompanied by paresthesia on his left lower labial mucosa. He had a previous history of similar lesion and had been treated with surgery and cauterization last year. The primary clinical impression was a recurrent mucocele. Microscopic surveys displayed a traumatic neuroma in the vicinity of a mucocele which seems to be arising from the previous surgical treatment.

  16. Persistent Postconcussive Symptoms Are Accompanied by Decreased Functional Brain Oxygenation.

    Science.gov (United States)

    Helmich, Ingo; Saluja, Rajeet S; Lausberg, Hedda; Kempe, Mathias; Furley, Philip; Berger, Alisa; Chen, Jen-Kai; Ptito, Alain

    2015-01-01

    Diagnostic methods are considered a major concern in the determination of mild traumatic brain injury. The authors examined brain oxygenation patterns in subjects with severe and minor persistent postconcussive difficulties and a healthy control group during working memory tasks in prefrontal brain regions using functional near-infrared spectroscopy. The results demonstrated decreased working memory performances among concussed subjects with severe postconcussive symptoms that were accompanied by decreased brain oxygenation patterns. An association appears to exist between decreased brain oxygenation, poor performance of working memory tasks, and increased symptom severity scores in subjects suffering from persistent postconcussive symptoms.

  17. Optic Neuritis Associated with Myelodysplastic Syndrome Accompanied by Eosinophilic Crisis.

    Science.gov (United States)

    Nagasaki, Joji; Nishimoto, Mitsutaka; Nakamae, Hirohisa; Nakane, Takahiko; Koh, Hideo; Yoshimoto, Kumiko; Shiraki, Kunihiko; Hino, Masayuki

    2015-01-01

    Myelodysplastic syndrome (MDS) was diagnosed in a 64-year-old man. Three months later, he presented with right-sided visual loss. A diagnosis of optic neuritis caused by both ischemic and non-ischemic changes was established. Concurrently, prominent eosinophilia was seen in both the peripheral blood and bone marrow. A partial improvement of visual loss was obtained concomitant with a rapid decrease of the eosinophils after treatment with corticosteroids. Optic neuritis related to MDS is a rare condition and its etiology has not yet been identified. We herein report a case of optic neuritis associated with MDS and accompanied by an eosinophilic crisis.

  18. Solutions manual to accompany finite mathematics models and applications

    CERN Document Server

    Morris, Carla C

    2015-01-01

    A solutions manual to accompany Finite Mathematics: Models and Applications In order to emphasize the main concepts of each chapter, Finite Mathematics: Models and Applications features plentiful pedagogical elements throughout such as special exercises, end notes, hints, select solutions, biographies of key mathematicians, boxed key principles, a glossary of important terms and topics, and an overview of use of technology. The book encourages the modeling of linear programs and their solutions and uses common computer software programs such as LINDO. In addition to extensive chapters on pr

  19. Value-sensitive clinical accompaniment in community nursing science

    Directory of Open Access Journals (Sweden)

    Ebin Arries

    2010-03-01

    Full Text Available Clinical community health facilities where undergraduate students are placed for their practical work in community nursing science are dynamic and have undergone major transformation over the past few years. In the clinical field, community nurses and undergraduate students are representative of the different races and language and ethnic groups in the South African population, with each group espousing different value systems. Both parties – students and community nurses – report that, due to these differences, value conflicts are experienced during clinical accompaniment and that this has negative effects on clinical learning in community nursing science.

    The goal of this study was to explore and describe the experiences of students with regard to value-sensitive clinical accompaniment in the community nursing environment. An exploratory, descriptive and contextual design was used. Interactions between community nurses and students during clinical accompaniment were explored for value sensitivity by means of video recordings,participant observation and focus group interviews. Data were collected by means of video recordings, participant observation and focus group interviews. The data were analysed and coded by the researcher and the external coder, using an inductive descriptive method to identify important segments of the regularity of behaviour. The focus group interviews were transcribed, analysed and coded by the researcher and the external coder, using Tesch’s steps of analysis (Creswell 1994:155–156.Lincoln and Guba’s criteria (1985:290 for trustworthiness were applied to the study.

    The general findings indicate that clinical accompaniment in community nursing is not value sensitive and, as a result, guidelines for value-sensitive clinical accompaniment need to be developed for undergraduate students in the community nursing environment. The following values (values for which guidelines need to be developed were

  20. Middleware Design for Swarm-Driving Robots Accompanying Humans.

    Science.gov (United States)

    Kim, Min Su; Kim, Sang Hyuck; Kang, Soon Ju

    2017-02-17

    Research on robots that accompany humans is being continuously studied. The Pet-Bot provides walking-assistance and object-carrying services without any specific controls through interaction between the robot and the human in real time. However, with Pet-Bot, there is a limit to the number of robots a user can use. If this limit is overcome, the Pet-Bot can provide services in more areas. Therefore, in this study, we propose a swarm-driving middleware design adopting the concept of a swarm, which provides effective parallel movement to allow multiple human-accompanying robots to accomplish a common purpose. The functions of middleware divide into three parts: a sequence manager for swarm process, a messaging manager, and a relative-location identification manager. This middleware processes the sequence of swarm-process of robots in the swarm through message exchanging using radio frequency (RF) communication of an IEEE 802.15.4 MAC protocol and manages an infrared (IR) communication module identifying relative location with IR signal strength. The swarm in this study is composed of the master interacting with the user and the slaves having no interaction with the user. This composition is intended to control the overall swarm in synchronization with the user activity, which is difficult to predict. We evaluate the accuracy of the relative-location estimation using IR communication, the response time of the slaves to a change in user activity, and the time to organize a network according to the number of slaves.

  1. 食管癌伴神经内分泌分化的临床病理特点及诊断标志物%Clinico-pathological characteristics and diagnostic biomarkers in patients with esophageal carcinoma accompanied by neuroendocrine cell differentiation

    Institute of Scientific and Technical Information of China (English)

    刘连科; 邵明雯; 马兰; 孙婧; 管丹; 束永前

    2015-01-01

    Objective To investigate the clinico-pathological characteristics and diagnostic markers in esophageal carci-noma accompanied by neuroendocrine cell differentiation (E-NED).Methods The clinico-pathological data of 378 patients with poorly differentiated esophageal carcinoma from Jan.2008 to Dec.2013,including 349 without neuroen-docrine differentiation (E-NNED)and 29 with neuroendocrine differentiation (E-NED),were retrospectively analyzed. The expressions of synaptophysin (SYN),chromogranin A (CgA),neuron-specific enolase (NSE),neural cell adhe-sion molecule (CD56),protein gene product (PGP9.5 ),secretagogin (SCGN)and thyroid transcription factor 1 (TTF-1)in the esophageal carcinoma tissues of 29 patients with E-NED and 20 ones randomly selected from the 349 patients with E-NNED were detected using immunohistochemistry.Results There were no significant differences between the patients with E-NED and E-NNED in terms of the age,gender,length of lesion and other clinico-pathologi-cal factors.Except for TTF-1,the expressions of Syn,CgA,NSE,CD56,PGP9.5 and SCGN were significantly higher in patients with E-NED than those with E-NNED (P diagnostic accuracy were all higher in the combined detection of Syn and CD56,PGP9.5 or SCGN than Syn and CgA (P <0.05).There were no significant differences between the patients with E-NED and E-NNED regarding 1,2 and 3-year survival rates.Conclusion E-NED and E-NNED may be different pathological types of esophageal carcinoma. Both PGP9.5 and SCGN can be considered as the diagnostic markers of E-NED,and the combined detection of Syn and CD56,PGP9.5 or SCGN can improve the sensitivity and diagnostic accuracy.%目的:探讨食管癌伴神经内分泌分化(E-NED)的临床病理特点及诊断标志物。方法回顾性分析2008年1月至2013年12月经病理证实的低分化食管癌378例患者的临床病理资料,其中无 NED(E-NNED)349例, E-NED 29例,免疫组织化学检测29例 E-NED 以及随机选取20例 E

  2. Treatment of lingual traumatic ulcer accompanied with fungal infections

    Directory of Open Access Journals (Sweden)

    Sella Sella

    2011-09-01

    Full Text Available Background: Traumatic ulcer is a common form of ulceration occured in oral cavity caused by mechanical trauma, either acute or chronic, resulting in loss of the entire epithelium. Traumatic ulcer often occurs in children that are usually found on buccal mucosa, labial mucosa of upper and lower lip, lateral tongue, and a variety of areas that may be bitten. To properly diagnose the ulcer, dentists should evaluate the history and clinical description in detail. If the lesion is allegedly accompanied by other infections, such as fungal, bacterial or viral infections, microbiological or serological tests will be required. One of the initial therapy given for fungal infection is nystatin which aimed to support the recovery and repair processes of epithelial tissue in traumatic ulcer case. Purpose: This case report is aimed to emphasize the importance of microbiological examination in suspected cases of ulcer accompanied with traumatic fungal infection. Case: A 12-year-old girl came to the clinic of Pediatric Dentistry, Faculty of Dentistry, University of Indonesia on June 9, 2011 accompanied with her mother. The patient who had a history of geographic tongue came with complaints of injury found in the middle of the tongue. The main diagnosis was ulcer accompanied with traumatic fungal infection based on the results of swab examination. Case management: This traumatic ulcer case was treated with Dental Health Education, oral prophylaxis, as well as prescribing and usage instructions of nystatin. The recovery and repair processes of mucosal epithelium of the tongue then occured after the use of nystatin. Conclusion: It can be concluded that microbiological examination is important to diagnose suspected cases of ulcer accompanied with traumatic fungal infection. The appropriate treatment such as nystatin can be given for traumatic fungal infection.Latar belakang: Ulkus traumatic merupakan bentuk umum dari ulserasi rongga mulut yang terjadi akibat trauma

  3. 19 CFR 133.24 - Restrictions on articles accompanying importer and mail importations.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Restrictions on articles accompanying importer and... accompanying importer and mail importations. (a) Detention. Articles accompanying an importer and mail... given in the following manner: (1) Articles accompanying importer. When the articles are carried...

  4. Neutronless $^{10}Be$-Accompanied Ternary Fission of $^{252}Cf$

    CERN Document Server

    Sandulescu, A; Misicu, S; Florescu, A; Ramayya, A V; Hamilton, J H; Greiner, W

    1998-01-01

    A new type of decay corresponding to the neutronless $^{10}$Be-accompanied fragmentation of $^{252}$Cf is studied. We employ a cluster model similar to the model used for the description of cluster radioactivity. No preformation factors were considered. The ternary relative isotopic yields were calculated as the ratio of the penetrability of a given ternary fragmentation over the sum of penetrabilities of all possible ternary neutronless fragmentations. The corresponding barriers between the light and heavy fragment and between the $^{10}$Be cluster and the two heavier fragments were computed with the help of a double folding potential generated by M3Y-$NN$ effective interaction and realistic fragment ground state deformations. Also, we studied the influence of the fragment excitation energies on the yields, by including the level densities and the $\\beta$-stretching of the fragments. The new phenomenon could be experimentally observed by the triple gamma coincidence technique between the fragments and $^{10}...

  5. Transient positive and negative experiences accompanying stage hypnosis.

    Science.gov (United States)

    Crawford, H J; Kitner-Triolo, M; Clarke, S W; Olesko, B

    1992-11-01

    Frequency of positive and negative experiences accompanying stage hypnosis was assessed in follow-up interviews with 22 participants of university-sponsored performances. Most subjects described their experience positively (relaxing, interesting, exciting, satisfying, illuminating, and pleasurable), but some described it negatively (confusing, silly, annoying, and frightening). Five subjects (22.7%) reported partial or complete amnesia; all were highly responsive to the stage hypnosis suggestions. One subject was completely unable to breach amnesia and felt annoyed and frightened. Five subjects (22.7%) believed the hypnotist had control over their behavior. Participants (n = 15) tested subsequently on the Stanford Hypnotic Susceptibility Scale, Form C (Weitzenhoffer & Hilgard, 1962) were mostly moderately to highly hypnotizable (M = 7.07), and the scores correlated significantly (r = .68) with the percentage of passed stage hypnosis suggestions.

  6. [Aspects of psychotherapy in diabetes mellitus with accompanying alcoholic intoxication].

    Science.gov (United States)

    Sidorov, P I; Novikova, I A; Solov'ev, A G

    2001-01-01

    120 patients with different types of diabetes mellitus were divided into groups by the presence and intensity of accompanying alcoholic intoxication: group 1--no alcohol intake; group 2--rare alcohol intake; group 3--moderate alcohol intake; group 4--alcoholic abuse. Experimental psychological methods were used together with psychotherapeutic approaches: Wiesbaden's questionnaire (WIPPF), Lusher's and MMPI tests. For each group of the patients some recommendations are given for application of the above techniques. Thus in patients from the 1-3 groups it is worthwhile to perform a positive psychotherapy directed to the development of the body/sensation sphere (according to WIPPF), that is a care about physical and mental state (including autotraining and training of communication). In the alcohol abusing patients the main point should be fantasy/future sphere (according to WIPPF) in the ranges of the same positive psychotherapy directed to forming positive world view and correction of the interpersonal relations.

  7. Lithium isotope effect accompanying electrochemical intercalation of lithium into graphite

    CERN Document Server

    Yanase, S; Oi, T

    2003-01-01

    Lithium has been electrochemically intercalated from a 1:2 (v/v) mixed solution of ethylene carbonate (EC) and methylethyl carbonate (MEC) containing 1 M LiClO sub 4 into graphite, and the lithium isotope fractionation accompanying the intercalation was observed. The lighter isotope was preferentially fractionated into graphite. The single-stage lithium isotope separation factor ranged from 1.007 to 1.025 at 25 C and depended little on the mole ratio of lithium to carbon of the lithium-graphite intercalation compounds (Li-GIC) formed. The separation factor increased with the relative content of lithium. This dependence seems consistent with the existence of an equilibrium isotope effect between the solvated lithium ion in the EC/MEC electrolyte solution and the lithium in graphite, and with the formation of a solid electrolyte interfaces on graphite at the early stage of intercalation. (orig.)

  8. Thermal expansion accompanying the glass-liquid transition and crystallization

    Directory of Open Access Journals (Sweden)

    M. Q. Jiang

    2015-12-01

    Full Text Available We report the linear thermal expansion behaviors of a Zr-based (Vitreloy 1 bulk metallic glass in its as-cast, annealed and crystallized states. Accompanying the glass-liquid transition, the as-cast Vitreloy 1 shows a continuous decrease in the thermal expansivity, whereas the annealed glass shows a sudden increase. The crystallized Vitreloy 1 exhibits an almost unchanged thermal expansivity prior to its melting. Furthermore, it is demonstrated that the nucleation of crystalline phases can induce a significant thermal shrinkage of the supercooled liquid, but with the growth of these nuclei, the thermal expansion again dominates. These results are explained in the framework of the potential energy landscape, advocating that the configurational and vibrational contributions to the thermal expansion of the glass depend on both, structure and temperature.

  9. ACCOMPANYING THERAPY IN PATIENTS AFTER ENDOSCOPIC LARYNGEAL SURGERY

    Directory of Open Access Journals (Sweden)

    E. N. Novozhilova

    2014-01-01

    Full Text Available The paper describes the experience with accompanying therapy in patients after endoscopic laryngeal surgery, which has been gained at the Unit of Head and Neck Tumors, Moscow City Cancer Hospital Sixty-Two. Endolaryngeal operations have been performed using robotic CO2 laser and alternative modes of mechanical ventilation. Methods for abolishing laser-induced reactive tissue changes with different groups of pharmaceuticals are considered. Both the possible side effects of some drugs and their potential interaction are taken into account. The high efficacy of current inhalation systems (PARI delivering the required doses of medicaments over a short period of time and with minimal losses and regulating their dispersion in relation to the drug used is noted.

  10. Comprehensive analysis of microorganisms accompanying human archaeological remains.

    Science.gov (United States)

    Philips, Anna; Stolarek, Ireneusz; Kuczkowska, Bogna; Juras, Anna; Handschuh, Luiza; Piontek, Janusz; Kozlowski, Piotr; Figlerowicz, Marek

    2017-07-01

    Metagenome analysis has become a common source of information about microbial communities that occupy a wide range of niches, including archaeological specimens. It has been shown that the vast majority of DNA extracted from ancient samples come from bacteria (presumably modern contaminants). However, characterization of microbial DNA accompanying human remains has never been done systematically for a wide range of different samples. We used metagenomic approaches to perform comparative analyses of microorganism communities present in 161 archaeological human remains. DNA samples were isolated from the teeth of human skeletons dated from 100 AD to 1200 AD. The skeletons were collected from 7 archaeological sites in Central Europe and stored under different conditions. The majority of identified microbes were ubiquitous environmental bacteria that most likely contaminated the host remains not long ago. We observed that the composition of microbial communities was sample-specific and not correlated with its temporal or geographical origin. Additionally, traces of bacteria and archaea typical for human oral/gut flora, as well as potential pathogens, were identified in two-thirds of the samples. The genetic material of human-related species, in contrast to the environmental species that accounted for the majority of identified bacteria, displayed DNA damage patterns comparable with endogenous human ancient DNA, which suggested that these microbes might have accompanied the individual before death. Our study showed that the microbiome observed in an individual sample is not reliant on the method or duration of sample storage. Moreover, shallow sequencing of DNA extracted from ancient specimens and subsequent bioinformatics analysis allowed both the identification of ancient microbial species, including potential pathogens, and their differentiation from contemporary species that colonized human remains more recently. © The Authors 2017. Published by Oxford University

  11. Brain-immune interaction accompanying odor-evoked autobiographic memory.

    Science.gov (United States)

    Matsunaga, Masahiro; Bai, Yu; Yamakawa, Kaori; Toyama, Asako; Kashiwagi, Mitsuyoshi; Fukuda, Kazuyuki; Oshida, Akiko; Sanada, Kazue; Fukuyama, Seisuke; Shinoda, Jun; Yamada, Jitsuhiro; Sadato, Norihiro; Ohira, Hideki

    2013-01-01

    The phenomenon in which a certain smell evokes a specific memory is known as the Proust phenomenon. Odor-evoked autobiographic memories are more emotional than those elicited by other sensory stimuli. The results of our previous study indicated that odor-evoked autobiographic memory accompanied by positive emotions has remarkable effects on various psychological and physiological activities, including the secretion of cytokines, which are immune-signaling molecules that modulate systemic inflammation. In this study, we aimed to clarify the neural substrates associated with the interaction between odor-evoked autobiographic memory and peripheral circulating cytokines. We recruited healthy male and female volunteers and investigated the association between brain responses and the concentration of several cytokines in the plasma by using positron emission tomography (PET) recordings when an autographic memory was evoked in participants by asking them to smell an odor that was nostalgic to them. Participants experienced positive emotions and autobiographic memories when nostalgic odors were presented to them. The levels of peripheral proinflammatory cytokines, such as the tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), were significantly reduced after experiencing odor-evoked autobiographic memory. Subtraction analysis of PET images indicated that the medial orbitofrontal cortex (mOFC) and precuneus/posterior cingulate cortex (PCC) were significantly activated during experiences of odor-evoked autobiographic memory. Furthermore, a correlation analysis indicated that activities of the mOFC and precuneus/PCC were negatively correlated with IFN-γ concentration. These results indicate that the neural networks including the precuneus/PCC and mOFC might regulate the secretion of peripheral proinflammatory cytokines during the experience of odor-evoked autobiographic memories accompanied with positive emotions.

  12. Middleware Design for Swarm-Driving Robots Accompanying Humans

    Science.gov (United States)

    Kim, Min Su; Kim, Sang Hyuck; Kang, Soon Ju

    2017-01-01

    Research on robots that accompany humans is being continuously studied. The Pet-Bot provides walking-assistance and object-carrying services without any specific controls through interaction between the robot and the human in real time. However, with Pet-Bot, there is a limit to the number of robots a user can use. If this limit is overcome, the Pet-Bot can provide services in more areas. Therefore, in this study, we propose a swarm-driving middleware design adopting the concept of a swarm, which provides effective parallel movement to allow multiple human-accompanying robots to accomplish a common purpose. The functions of middleware divide into three parts: a sequence manager for swarm process, a messaging manager, and a relative-location identification manager. This middleware processes the sequence of swarm-process of robots in the swarm through message exchanging using radio frequency (RF) communication of an IEEE 802.15.4 MAC protocol and manages an infrared (IR) communication module identifying relative location with IR signal strength. The swarm in this study is composed of the master interacting with the user and the slaves having no interaction with the user. This composition is intended to control the overall swarm in synchronization with the user activity, which is difficult to predict. We evaluate the accuracy of the relative-location estimation using IR communication, the response time of the slaves to a change in user activity, and the time to organize a network according to the number of slaves. PMID:28218650

  13. TMJ degeneration in SAMP8 mice is accompanied by deranged Ihh signaling.

    Science.gov (United States)

    Ishizuka, Y; Shibukawa, Y; Nagayama, M; Decker, R; Kinumatsu, T; Saito, A; Pacifici, M; Koyama, E

    2014-03-01

    The temporomandibular joint (TMJ) functions as a load-bearing diarthrodial joint during mastication, and its continuous use and stress can lead to degeneration over age. Using senescence-accelerated (SAMP8) mice that develop early osteoarthritis-like changes in synovial joints at high frequency, we analyzed possible molecular mechanisms of TMJ degeneration and tested whether and how malocclusion may accelerate it. Condylar articular cartilage in young SAMP8 mice displayed early-onset osteoarthritic changes that included reductions in superficial/chondroprogenitor cell number, proteoglycan/collagen content, and Indian hedgehog (Ihh)-expressing chondrocytes. Following malocclusion induced by tooth milling, the SAMP8 condyles became morphologically defective, displayed even lower proteoglycan levels, and underwent abnormal chondrocyte maturation compared with malocclusion-treated condyles in wild-type mice. Malocclusion also induced faster progression of pathologic changes with increasing age in SAMP8 condyles as indicated by decreased PCNA-positive proliferating chondroprogenitors and increased TUNEL-positive apoptotic cells. These changes were accompanied by steeper reductions in Ihh signaling and by expression of matrix metalloproteinase 13 at the chondro-osseous junction in SAMP8 articular cartilage. In sum, we show for the first time that precocious TMJ degeneration in SAMP8 mice is accompanied by--and possibly attributable to--altered Ihh signaling and that occlusal dysfunction accelerates progression toward degenerative TMJ disease in this model.

  14. Effects of SCR-3 on the immunosuppression accompanied with the systemic inflammatory response syndrome.

    Science.gov (United States)

    Li, Jun; Niu, Jie; Ou, Shan; Ye, Zhan-Yong; Liu, Deng-Qun; Wang, Feng-Chao; Su, Yong-Ping; Wang, Jun-Ping

    2012-05-01

    Steroid receptor coactivator-3 (SRC-3) is a multifunctional protein that plays an important role in mammary gland growth, development, and tumorigenesis. In this study, SCR-3 gene knockout mice were used to study the effects of SCR-3 on the immunosuppression accompanied with systemic inflammatory response syndrome (SIRS). Bacterial clearance assay was performed by blood culture and frozen sections, and the results showed that the absence of SCR-3 protein serious damaged the innate immune system and the body's ability to inactivate or phagocytosis of bacteria was significantly decreased, and the absence of SCR-3 protein also weakened phagocytes' ability to degrade bacteria and their metabolites. Furthermore, animal model of inflammatory reaction was established and the immune function was determined, and the results revealed that SRC-3 protein may play an important role in maintenance of T-cells' immune function, and severe T-cell immune function disorder would be resulted once SRC-3 protein is missing. In addition, the results of our study showed the steady-state of lymphocyte subsets was destroyed after SIRS, leading the suppression of cellular immune function, and the absence of SCR-3 protein may aggravate the suppression of T-lymphocyte function. Therefore, the present study demonstrated that the absence of SCR-3 protein would aggravate immunosuppression. In addition, SRC-3 protein is a significant regulator of infection and inflammation, and SRC-3 protein play an essential role in the development of immunosuppression accompanied with SIRS.

  15. Soundtracks to Accompany Visualizations of Nuclear Pasta Simulations

    Science.gov (United States)

    Clark, Emily

    2014-09-01

    Nuclear pasta is a substance found in neutron stars and core-collapse supernovae, arising at the extreme densities near nuclear saturation, when the attractive nuclear and repulsive coulomb forces mold the dense sea of protons and neutrons into shapes such as spheres, tubes, and slabs, which somewhat resemble different types of pasta. The structures are analyzed using molecular dynamical simulations for different proton fractions, temperatures, densities, and number of nucleons. The system is stressed by stretching it, squeezing it, or subjecting it to some outside force. In order to obtain a more complete representation of how the nuclear pasta responds, sound tracks were produced to accompany videos of stretching simulations. The audio tracks were made by assuming sound waves are produced from changes in the nucleon density. This density was calculated within a small region at frequent time intervals during the run. The resulting sound track was then synced with a video of the run in order to emphasize the development of the system as the pasta moves and breaks. Nuclear pasta is a substance found in neutron stars and core-collapse supernovae, arising at the extreme densities near nuclear saturation, when the attractive nuclear and repulsive coulomb forces mold the dense sea of protons and neutrons into shapes such as spheres, tubes, and slabs, which somewhat resemble different types of pasta. The structures are analyzed using molecular dynamical simulations for different proton fractions, temperatures, densities, and number of nucleons. The system is stressed by stretching it, squeezing it, or subjecting it to some outside force. In order to obtain a more complete representation of how the nuclear pasta responds, sound tracks were produced to accompany videos of stretching simulations. The audio tracks were made by assuming sound waves are produced from changes in the nucleon density. This density was calculated within a small region at frequent time intervals

  16. Reactivation from latency displays HIV particle budding at plasma membrane, accompanying CD44 upregulation and recruitment

    Directory of Open Access Journals (Sweden)

    Sano Kouichi

    2009-07-01

    Full Text Available Abstract Background It has been accepted that HIV buds from the cell surface in T lymphocytes, whereas in macrophages it buds into intracellular endosomes. Recent studies, on the other hand, suggest that HIV preferentially buds from the cell surface even in monocytic cells. However, most studies are based on observations in acutely infected cells and little is known about HIV budding concomitant with reactivation from latency. Such studies would provide a better understanding of a reservoir for HIV. Results We observed HIV budding in latently infected T lymphocytic and monocytic cell lines following TNF-α stimulation and examined the upregulation of host factors that may be involved in particle production. Electron microscopy analysis revealed that reactivation of latently infected J1.1 cells (latently infected Jurkat cells with HIV-1 and U1 cells (latently infected U937 cells with HIV-1 displayed HIV particle budding predominantly at the plasma membrane, a morphology that is similar to particle budding in acutely infected Jurkat and U937 cells. When mRNA expression levels were quantified by qRT-PCR, we found that particle production from reactivated J1.1 and U1 cells was accompanied by CD44 upregulation. This upregulation was similarly observed when Jurkat and U937 cells were acutely infected with HIV-1 but not when just stimulated with TNF-α, suggesting that CD44 upregulation was linked with HIV production but not with cell stimulation. The molecules in endocytic pathways such as CD63 and HRS were also upregulated when U1 cells were reactivated and U937 cells were acutely infected with HIV-1. Confocal microscopy revealed that these upregulated host molecules were recruited to and accumulated at the sites where mature particles were formed at the plasma membrane. Conclusion Our study indicates that HIV particles are budded at the plasma membrane upon reactivation from latency, a morphology that is similar to particle budding in acute

  17. Melatonin production accompanies arousal from daily torpor in Siberian hamsters.

    Science.gov (United States)

    Larkin, Jennie E; Yellon, Steven M; Zucker, Irving

    2003-01-01

    Arousal from deep hibernation is accompanied by a transient rise of melatonin (Mel) in circulation; there are no comparable analyses of Mel concentrations in species that undergo much shallower, shorter duration episodes of daily torpor. Serum Mel concentrations were determined during arousal from both natural daily torpor and torpor induced by 2-deoxy-D-glucose (2-DG) treatment (2,500 mg/kg, intraperitoneal [IP]); blood samples were drawn from the retro-orbital sinus of anesthetized Siberian hamsters. For animals kept in darkness during torpor, Mel concentrations were highest during early arousal when thermogenesis is maximal, and they decreased as body temperature increased during arousal and returned to baseline once euthermia was reestablished. In hamsters kept in the light during the torpor bout, Mel concentrations were elevated above basal values during arousal, but the response was significantly blunted in comparison with values recorded in darkness. Increased Mel concentrations were detected in hamsters only during arousal from torpor (either natural or 2-DG induced) and were not simply a result of the drug treatment; hamsters that remained euthermic or manifested mild hypothermia after drug treatment maintained basal Mel concentrations. We propose that increased Mel production may reflect enhanced sympathetic activation associated with intense thermogenesis during arousal from torpor rather than an adjustment of the circadian rhythm of Mel secretion.

  18. The evolution of aposematism is accompanied by increased diversification.

    Science.gov (United States)

    Przeczek, Kara; Mueller, Carla; Vamosi, Steven M

    2008-09-01

    Although the use of distinct colors to indicate unprofitability to predators (i.e. aposematism) is commonly thought of as an adaptation, our knowledge of its macroevolutionary effects is limited. Because aposematism is expected to decrease attacks by predators, we hypothesized that aposematic lineages should be larger on average than their non-aposematic sister groups because of the decreased probability of extinction and/or increased probability of speciation (i.e. increased diversification). The results of our sister-group analysis are consistent with the hypothesis that the evolution of aposematism is accompanied by increased diversification of lineages, with the aposematic focal group having more species in 11 of 14 pairs of sister groups. Despite the apparent advantages of reduced predation risk on diversification rates, the evolution of aposematism is relatively rare and reversions to a cryptic state are not uncommon. In addition to the difficulties in evolving a trait that initially decreases the survivorship of prey among naive predators, we discuss other factors that may limit the apparent prevalence and success of aposematism. It is hoped that the results of our analysis will encourage further analyses of the phylogenetic relationships among aposematic groups and their relatives, and of the evolutionary time scales over which the benefits of aposematism are the greatest to lineages with this condition. © 2008 ISZS, Blackwell Publishing and IOZ/CAS.

  19. Radio rebrightening of the GRB afterglow by the accompanying supernova

    CERN Document Server

    Duran, Rodolfo Barniol

    2015-01-01

    The gamma-ray burst (GRB) jet powers the afterglow emission by shocking the surrounding medium, and radio afterglow can now be routinely observed to almost a year after the explosion. Long-duration GRBs are accompanied by supernovae (SNe) that typically contain much more energy than the GRB jet. Here we consider the fact that the SN blast wave will also produce its own afterglow, which will peak at much later time (since it is non-relativistic), when the SN blast wave transitions from a coasting phase to a decelerating Sedov-Taylor phase. We predict that this component will peak generally a few tens of years after the explosion and it will outshine the GRB powered afterglow well-before its peak emission. In the case of GRB 030329, where the external density is constrained by the $\\sim 10$-year coverage of the radio GRB afterglow, the radio emission is predicted to start rising over the next decade and to continue to increase for the following decades up to a level of $\\sim 0.5$ mJy. Detection of the SN-powere...

  20. Heat transfer enhancement accompanying Leidenfrost state suppression at ultrahigh temperatures.

    Science.gov (United States)

    Shahriari, Arjang; Wurz, Jillian; Bahadur, Vaibhav

    2014-10-14

    The well-known Leidenfrost effect is the formation of a vapor layer between a liquid and an underlying hot surface. This insulating vapor layer severely degrades heat transfer and results in surface dryout. We measure the heat transfer enhancement and dryout prevention benefits accompanying electrostatic suppression of the Leidenfrost state. Interfacial electric fields in the vapor layer can attract liquid toward the surface and promote wetting. This principle can suppress dryout even at ultrahigh temperatures exceeding 500 °C, which is more than 8 times the Leidenfrost superheat for organic solvents. Robust Leidenfrost state suppression is observed for a variety of liquids, ranging from low electrical conductivity organic solvents to electrically conducting salt solutions. Elimination of the vapor layer increases heat dissipation capacity by more than 1 order of magnitude. Heat removal capacities exceeding 500 W/cm(2) are measured, which is 5 times the critical heat flux (CHF) of water on common engineering surfaces. Furthermore, the heat transfer rate can be electrically controlled by the applied voltage. The underlying science is explained via a multiphysics analytical model which captures the coupled electrostatic-fluid-thermal transport phenomena underlying electrostatic Leidenfrost state suppression. Overall, this work uncovers the physics underlying dryout prevention and demonstrates electrically tunable boiling heat transfer with ultralow power consumption.

  1. Ophthalmic manifestations of methylmalonic aciduria accompanied with homocystinuria

    Directory of Open Access Journals (Sweden)

    Qiu-Jing Huang

    2015-12-01

    Full Text Available Methylmalonicaciduia(MMAaccompanied with homocystinuria is a rare autosomal-recessive with congenital metabolic disorder of Vitamin B12. There are three subtypes, cblC, cblD, cblF, in which cblC is the most common one. The diagnostic tests are tandem mass spectrometry and gas chromatography-mass spectrometry. Tests for activity of enzyme in fibroblasts from skin, complementary assay and genetic analysis can be used to make the subtype clear. Early-onset patients, defined by onset of symptoms before the age of 1 year, may have severe ocular involvement, including visual loss, nystagmus, strabismus, retinopathy, maculopathy, optic atrophy, abnormal electroretinography. Late-onset patients, defined by onset of symptoms after the age of 4 year, rarely have ocular manifestations. The pathogenesis of the ophthalmic symptoms may be related to the high level of homocystine, oxidative stress and the abnormal development of nervous systems. The treatment for MMA accompanied with homocystinuria is mostly symptomatic based. Ophthalmic treatment is limited. Early supplement of methionine,GSH or other antioxidants may be helpful for retinopathy. There is no standard ophthalmological examination for those patients in China. It is critical to set up inter-departmental cooperation and early stage examination for the treatments and outcomes of the patients.

  2. Hemiplegic peripheral neuropathy accompanied with multiple cranial nerve palsy

    Directory of Open Access Journals (Sweden)

    Hirohisa Okuma

    2012-03-01

    Full Text Available A 32-year-old man experienced double vision around January, 2010, followed by weakness of his left upper and lower extremities. Articulation disorders and loss of hearing in his left ear developed, and he was admitted to our hospital on February 14, 2010. Physical examination was normal, and neurological examination showed clear consciousness with no impairment of cognitive function, but with articulation disorders. Olfactory sensation was reduced. Left ptosis and left gaze palsy, complete left facial palsy, perceptive deafness of the left ear, and muscle weakness of the left trapezius muscle were observed. Paresis in the left upper and lower extremities was graded 4/5 through manual muscle testing. Sensory system evaluation revealed complete left-side palsy, including the face. Deep tendon reflexes were slightly diminished equally on both sides; no pathologic reflex was seen. No abnormality of the brain parenchyma, cerebral nerves or cervicothoracolumbar region was found on brain magnetic resonance imaging. On electroencephalogram, alpha waves in the main frequency band of 8 to 9 Hz were recorded, indicating normal findings. Brain single photon emission computed tomography (SPECT scan showed reduced blood flow in the right inner frontal lobe and both occipital lobes. Nerve biopsy (left sural nerve showed reduction of nerve density by 30%, with demyelination. The patient also showed manifestations of multiple cranial nerve disorder, i.e., of the trigeminal nerve, glossopharyngeal nerve, vagus nerve, and hypoglossal nerve. Whole-body examination was negative. Finally, based on ischemic brain SPECT images, spinal fluid findings and nerve biopsy results, peripheral neuropathy accompanied with multiple cranial nerve palsy was diagnosed.

  3. Analgesia accompanying food consumption requires ingestion of hedonic foods.

    Science.gov (United States)

    Foo, H; Mason, Peggy

    2009-10-14

    Animals eat rather than react to moderate pain. Here, we examined the behavioral, hedonic, and neural requirements for ingestion analgesia in ad libitum fed rats. Noxious heat-evoked withdrawals were similarly suppressed during self-initiated chocolate eating and ingestion of intraorally infused water, sucrose, or saccharin, demonstrating that ingestion analgesia does not require feeding motivation, self-initiated food procurement, sucrose, or calories. Rather, food hedonics is important because neither salt ingestion nor quinine rejection elicited analgesia. During quinine-induced nausea and lipopolysaccharide (LPS)-induced illness, conditions when chocolate eating was presumably less pleasurable, analgesia accompanying chocolate consumption was attenuated, yet analgesia during water ingestion was preserved in LPS-injected rats who showed enhanced palatability for water within this context. The dependence of ingestion analgesia on the positive hedonics of an ingestate was confirmed in rats with a conditioned taste aversion to sucrose: after paired exposure to sucrose and LPS, rats no longer showed analgesia during sucrose ingestion but continued to show analgesia during chocolate consumption. Eating pauses tended to occur less often and for shorter durations in the presence of ingestion analgesia than in its absence. Therefore, we propose that ingestion analgesia functions to defend eating from ending. Muscimol inactivation of the medullary raphe magnus blocked the analgesia normally observed during water ingestion, showing the involvement of brainstem endogenous pain inhibitory mechanisms in ingestion analgesia. Brainstem-mediated defense of the consumption of palatable foods may explain, at least in part, why overeating tasty foods is so irresistible even in the face of opposing cognitive and motivational forces.

  4. EEG signatures accompanying auditory figure-ground segregation.

    Science.gov (United States)

    Tóth, Brigitta; Kocsis, Zsuzsanna; Háden, Gábor P; Szerafin, Ágnes; Shinn-Cunningham, Barbara G; Winkler, István

    2016-11-01

    In everyday acoustic scenes, figure-ground segregation typically requires one to group together sound elements over both time and frequency. Electroencephalogram was recorded while listeners detected repeating tonal complexes composed of a random set of pure tones within stimuli consisting of randomly varying tonal elements. The repeating pattern was perceived as a figure over the randomly changing background. It was found that detection performance improved both as the number of pure tones making up each repeated complex (figure coherence) increased, and as the number of repeated complexes (duration) increased - i.e., detection was easier when either the spectral or temporal structure of the figure was enhanced. Figure detection was accompanied by the elicitation of the object related negativity (ORN) and the P400 event-related potentials (ERPs), which have been previously shown to be evoked by the presence of two concurrent sounds. Both ERP components had generators within and outside of auditory cortex. The amplitudes of the ORN and the P400 increased with both figure coherence and figure duration. However, only the P400 amplitude correlated with detection performance. These results suggest that 1) the ORN and P400 reflect processes involved in detecting the emergence of a new auditory object in the presence of other concurrent auditory objects; 2) the ORN corresponds to the likelihood of the presence of two or more concurrent sound objects, whereas the P400 reflects the perceptual recognition of the presence of multiple auditory objects and/or preparation for reporting the detection of a target object.

  5. International accompaniment in violent scenarios. A performative reading of Peace Brigades International in Colombia

    OpenAIRE

    Fabra Mata, Javier

    2006-01-01

    Since its first application in Central America in the 1980s, the practice of nonviolent international accompaniment of civil society members threatened with political violence has undergone substantial growth. This thesis provides an understanding of protective international accompaniment as a communicative phenomenon. It shows the intricate dynamics of international accompaniment and its impact on two major interlocutors: the host government and the accompanied, under a discursive focus ...

  6. Magnetic Resonance Imaging of Mitochondrial Dysfunction and Metabolic Activity, Accompanied by Overproduction of Superoxide.

    Science.gov (United States)

    Bakalova, Rumiana; Georgieva, Ekaterina; Ivanova, Donika; Zhelev, Zhivko; Aoki, Ichio; Saga, Tsuneo

    2015-12-16

    This study shows that a mitochondria-penetrating nitroxide probe (mito-TEMPO) allows detection of superoxide and visualization of mitochondrial dysfunction in living cells due to the effect of T1 shortening in MRI. Mitochondrial dysfunction was induced by treatment of cells with rotenone and 2-methoxyestradiol (2-ME/Rot). The MRI measurements were performed on 7T MRI. The 2-ME/Rot-treated cells were characterized by overproduction of superoxide, which was confirmed by a conventional dihydroethidium test. In the presence of mito-TEMPO, the intensity of MRI signal in 2-ME/Rot-treated cells was ∼30-40% higher, in comparison with that in untreated cells or culture media. In model (cell-free) systems, we observed that superoxide, but not hydrogen peroxide, increased the intensity of T1-weighted MRI signal of mito-TEMPO. Moreover, the superoxide restores the T1-weighted MRI contrast of mito-TEMPOH, a noncontrast (diamagnetic) analogue of mito-TEMPO. This was also confirmed by using EPR spectroscopy. The results demonstrate that superoxide radical is involved in the enhancement of T1-weighted MRI contrast in living cells, in the absence and presence of mito-TEMPO. This report gives a direction for discovering new opportunities for functional MRI, for detection of metabolic activity, accompanied by overproduction of superoxide, as well as by disturbance of the balance between superoxide and hydrogen peroxide, a very important approach to clarify the fine molecular mechanisms in the regulation of many pathologies. The visualization of mitochondrial activity in real-time can be crucial to clarify the molecular mechanism of the functional MRI in its commonly accepted definition, as a method for detection of neurovascular coupling.

  7. Research on the dry intrusion accompanying the low vortex precipitation

    Institute of Scientific and Technical Information of China (English)

    YAO; XiuPing; WU; GuoXiong; ZHAO; BingKe; YU; YuBin; YANG; GuiMing

    2007-01-01

    By employing the 6.7μm satellite vapor cloud images and NCEP/NCAR 1°×1° reanalysis datasets, the characteristics and mechanism of the dry intrusion, as well as its impacts on the low vortex precipitation at the Meiyu front are explored in this paper. It is found that the formation,development and maintenance of the low vortex precipitation at the Meiyu front are closely related to the evolution of the dry intrusion. The dry intrusion is characterized by high potential vorticity (PV), low humidity and cold air. The dry intrusion exhibits as an obvious dark zone on vapor cloud images, an area in which atmospheric relative humidity is lower than 60%. However, the features of the dry intrusion on the vapor images are clearer than that of the humidity field, for the former is the digital vapor cloud images with high temporal and spatial resolution, and it can be used to explore the finer characteristics of the development, evolution and supplement of the intrusion during the development of the low vortex. The dry intrusion impacts accompanying the low vortex precipitation at the Meiyu front come from all levels of the troposphere, with the strongest intrusion located at the upper troposphere. The dry and cold air intrudes the vicinity of the low vortex from the upper isentropic surface to the lower one, slanting east-ward from lower to higher level. The low vortex precipitation region is usually situated in front of the dry intrusion where the relative humidity gradient is higher. The research also reveals that the mechanism of the dry intrusion is that the high potential vorticity descends from the upper troposphere to the lower level, therefore, the dry intrusion can be used as an important index of the high PV forcing. To the west of the low vortex precipitation, the upper level northerlies descend across the isentropic surface, then the dry cold advection can trigger the instable development in the midlow troposphere. The dry intru-sion enhances the low vortex

  8. Critical transition in tissue homeostasis accompanies murine lung senescence.

    Directory of Open Access Journals (Sweden)

    Carla L Calvi

    Full Text Available BACKGROUND: Respiratory dysfunction is a major contributor to morbidity and mortality in aged populations. The susceptibility to pulmonary insults is attributed to "low pulmonary reserve", ostensibly reflecting a combination of age-related musculoskeletal, immunologic and intrinsic pulmonary dysfunction. METHODS/PRINCIPAL FINDINGS: Using a murine model of the aging lung, senescent DBA/2 mice, we correlated a longitudinal survey of airspace size and injury measures with a transcriptome from the aging lung at 2, 4, 8, 12, 16 and 20 months of age. Morphometric analysis demonstrated a nonlinear pattern of airspace caliber enlargement with a critical transition occurring between 8 and 12 months of age marked by an initial increase in oxidative stress, cell death and elastase activation which is soon followed by inflammatory cell infiltration, immune complex deposition and the onset of airspace enlargement. The temporally correlative transcriptome showed exuberant induction of immunoglobulin genes coincident with airspace enlargement. Immunohistochemistry, ELISA analysis and flow cytometry demonstrated increased immunoglobulin deposition in the lung associated with a contemporaneous increase in activated B-cells expressing high levels of TLR4 (toll receptor 4 and CD86 and macrophages during midlife. These midlife changes culminate in progressive airspace enlargement during late life stages. CONCLUSION/SIGNIFICANCE: Our findings establish that a tissue-specific aging program is evident during a presenescent interval which involves early oxidative stress, cell death and elastase activation, followed by B lymphocyte and macrophage expansion/activation. This sequence heralds the progression to overt airspace enlargement in the aged lung. These signature events, during middle age, indicate that early stages of the aging immune system may have important correlates in the maintenance of tissue morphology. We further show that time-course analyses of aging

  9. Autoimmune myelofibrosis accompanied by Sjögren's syndrome in a 47, XXX/46, XX mosaic woman.

    Science.gov (United States)

    Takahashi, Tohru

    2014-01-01

    This report describes a patient with autoimmune myelofibrosis accompanied by Sjögren's syndrome (SS). A 36-year-old woman was admitted due to petechiae, purpura, gingival bleeding, dyspnea on exertion, and a lack of concentration. She had pancytopenia and was diagnosed with SS. A bone marrow study showed hypercellular marrow with reticulin fibrosis. Lymphocytic infiltrates and aggregates composed of a mixture of T and B cells in the marrow were also observed. A chromosomal analysis of the marrow cells showed 47, XXX and an analysis of peripheral lymphocytes revealed 47, XXX/46, XX mosaic results. The patient's cytopenia resolved following treatment with oral prednisolone.

  10. Neuromodulation of Limb Proprioceptive Afferents Decreases Apnea of Prematurity and Accompanying Intermittent Hypoxia and Bradycardia.

    Directory of Open Access Journals (Sweden)

    Kalpashri Kesavan

    Full Text Available Apnea of Prematurity (AOP is common, affecting the majority of infants born at <34 weeks gestational age. Apnea and periodic breathing are accompanied by intermittent hypoxia (IH. Animal and human studies demonstrate that IH exposure contributes to multiple pathologies, including retinopathy of prematurity (ROP, injury to sympathetic ganglia regulating cardiovascular action, impaired pancreatic islet cell and bone development, cerebellar injury, and neurodevelopmental disabilities. Current standard of care for AOP/IH includes prone positioning, positive pressure ventilation, and methylxanthine therapy; these interventions are inadequate, and not optimal for early development.The objective is to support breathing in premature infants by using a simple, non-invasive vibratory device placed over limb proprioceptor fibers, an intervention using the principle that limb movements trigger reflexive facilitation of breathing.Premature infants (23-34 wks gestational age, with clinical evidence of AOP/IH episodes were enrolled 1 week after birth. Caffeine treatment was not a reason for exclusion. Small vibration devices were placed on one hand and one foot and activated in 6 hour ON/OFF sequences for a total of 24 hours. Heart rate, respiratory rate, oxygen saturation (SpO2, and breathing pauses were continuously collected.Fewer respiratory pauses occurred during vibration periods, relative to baseline (p<0.005. Significantly fewer SpO2 declines occurred with vibration (p<0.05, relative to control periods. Significantly fewer bradycardic events occurred during vibration periods, relative to no vibration periods (p<0.05.In premature neonates, limb proprioceptive stimulation, simulating limb movement, reduces breathing pauses and IH episodes, and lowers the number of bradycardic events that accompany aberrant breathing episodes. This low-cost neuromodulatory procedure has the potential to provide a non-invasive intervention to reduce apnea, bradycardia and

  11. Spontaneous necrosis of solid gallbladder adenocarcinoma accompanied with pancreaticobiliary maljunction

    Institute of Scientific and Technical Information of China (English)

    Tomohide Hori; Takashi Wagata; Kenji Takemoto; Takanobu Shigeta; Haruko Takuwa; Koichiro Hata; Shinji Uemoto; Naoki Yokoo

    2008-01-01

    A 71-year-old Japanese man with acute cholecystitis and an incarcerated gallbladder (GB) stone was admitted. Plain ultrasonography (US) incidentally detected a mass-like lesion in the fundus. Doppler US revealed that this elevated lesion had no blood flow. Computed tomography showed a relatively low-density mass, measuring 5 cm×4 cm in diameter, with no positive enhancement. Magnetic resonance imaging showed a mass in the fundus with a slightly low intensity on T1-weighted images and a slightly high intensity on T2-weighted images. We were agonized in making the qualitative diagnosis of mass-like lesions of the fundus, such as a benign tumor, cancer, or debris. We performed laparoscopic cholecystectomy, because the incarcerated GB stone clearly caused acute cholecystitis. Intra-operative cholangiography clearly revealed pancreaticobiliary maljunction. Amylase levels in the common bile duct and gallbladder were quite high. The elevated lesion in the fundus clearly showed severe necrosis. Although this necrotic nodule included non-viable adenocarcinoma cells, viable cancer cell nests were located in the muscularis propria and subcutaneous layer. Histopathological examination confirmed a solid adenocarcinoma. Thus, we diagnosed it as a gallbladder cancer, based on histopathological analysis of the resected specimen. We therefore undertook radical surgery, including wedge resection of the liver, radical dissection of regional lymph nodes, and resection of the extrahepatic bile duct. Histopathological findings revealed no cancer, hyperplasia or dysplasia in the additionally resected specimens. The patient was finally staged as T2, NO, H0, P0, M(-), stage 11. We present the first case of spontaneous necrosis of solid gallbladder adenocarcinoma, with a review of previous studies.

  12. V-region-related and -unrelated immunosupression accompanying infections

    Directory of Open Access Journals (Sweden)

    Mario Arala-Chaves

    1992-01-01

    Full Text Available This paper discusses current evidence for the relationship between polyclonal lymphocyte activation, specific immunossupression with decreased resistance, and autoimmune pathology, that are all often found associated with infections by a variety of virus, bacteria and parasites . The central question of class determination of immune effector activities is considered in the context of the cellular targets for nonspecific mitogenic activities associated with infection. A model is presented to integrate these findings: mitogenens produced by the microorganism or the infected cells are preferentially active on CD5 B cells, the resulting over-production of IL-10 will tend to bias all immune activities in to a Th-2mode of effector functions, with high titers of polyclonal antibodies and litle or no production of gamma IFN and other "inflamatory"lymphokines that often mediate resistance. In turn these conditions allow for parasite persistence and the corresponding long-term disregulation of self-directed immune reactivities, resulting in autoimmunity in the chronic phase. This model would predict that selective immunization with the mitogenic principles involved in desregulation, could stand better chances than strategies of vaccination based on immunopotentiation against othere, functionally neutral antigenic epitopes. It is argued, however, that the complexity of immune responses and their regulation together with our ignorance on the genetic controls of class-determination, offer poor prospects for a scientifically-based, rational development of vaccines in the near future. It is suggested that empirically-based and technologically developed vaccines might suceed, while basic scientific approaches are reinforced and given the time provide a better understanding of those process.

  13. LTP is accompanied by an enhanced local excitability of pyramidal neuron dendrites.

    Science.gov (United States)

    Frick, Andreas; Magee, Jeffrey; Johnston, Daniel

    2004-02-01

    The propagation and integration of signals in the dendrites of pyramidal neurons is regulated, in part, by the distribution and biophysical properties of voltage-gated ion channels. It is thus possible that any modification of these channels in a specific part of the dendritic tree might locally alter these signaling processes. Using dendritic and somatic whole-cell recordings, combined with calcium imaging in rat hippocampal slices, we found that the induction of long-term potentiation (LTP) was accompanied by a local increase in dendritic excitability that was dependent on the activation of NMDA receptors. These changes favored the back-propagation of action potentials into this dendritic region with a subsequent boost in the Ca(2+) influx. Dendritic cell-attached patch recordings revealed a hyperpolarized shift in the inactivation curve of transient, A-type K(+) currents that can account for the enhanced excitability. These results suggest an important mechanism associated with LTP for shaping signal processing and controlling dendritic function.

  14. Chronic myelogenous leukemia accompanied by megaloblastic anemia showing atypical clinical features.

    Science.gov (United States)

    Koiso, Hiromi; Tsukamoto, Norifumi; Shimano, Shun-ichi; Karasawa, Masamitsu; Murakami, Hirokazu; Nojima, Yoshihisa

    2011-11-01

    Leukocytosis, splenomegaly, and an increased vitamin B(12) level are characteristic findings of chronic myelogenous leukemia in the chronic phase (CML-CP). Here, we report a patient with CML-CP accompanied by megaloblastic anemia. A 61-year-old man consulted our hospital because of anemia and thrombocytopenia. On physical examination, there were no remarkable findings; there was no hepatosplenomegaly. Laboratory findings were: hemoglobin 6.0 g/dl; MCV 113.6 fl; platelet count 100×10(9)/l; white cell count 8.66×10(9)/l; and LDH 1,236 IU/l. Peripheral blood smear demonstrated hypersegmented neutrophils and megalocytes with emergence of myeloblasts, giant metamyelocytes, and nucleated red cells. Vitamin B(12) and folic acid levels were low. Bone marrow examination showed megaloblastic change in the erythroblasts and myeloid hyperplasia. Following vitamin B(12) and folic acid administration, anemia and thrombocytopenia rapidly improved; thereafter, marked leukocytosis became evident. Based on the presence of t(9;22)(q34;q11) on cytogenetic study and a positive result for Major bcr/abl fusion gene, a diagnosis of CML-CP was established. This case illustrates that ineffective erythropoiesis results in anemia and thrombocytopenia in CML with vitamin B12 and/or folic acid deficiency.

  15. A Case of Eosinophilic Esophagitis Accompanying Familial Mediterranean Fever

    Directory of Open Access Journals (Sweden)

    Pejman Rohani

    2017-01-01

    Full Text Available Background. Eosinophilic esophagitis is an inflammatory condition where there is a dense infiltration of eosinophils typically exceeding fifteen cells per high power field. Familial Mediterranean fever is an autosomal recessive disorder characterized by brief, acute, and self-limited episodes of fever and polyserositis that recur at irregular intervals. Case Presentation. A three-year-and-nine-month-old Iranian girl was admitted to our center. The patient’s parents complained of a history of abdominal pain, poor appetite, and poor weight gain from 1.5 years ago and episodes of food impaction after starting solid foods. Eosinophilic esophagitis was diagnosed based on histology. Because of continuing abdominal pain after treatment of eosinophilic esophagitis, the episodic nature of disease, and the presence of fever with pain, screening for familial Mediterranean fever mutation was performed and the patient was found to be heterozygote for Mediterranean fever. Conclusion. We have reported a case of eosinophilic esophagitis coexisting with familial Mediterranean fever which has not been described previously.

  16. A Case of Eosinophilic Esophagitis Accompanying Familial Mediterranean Fever.

    Science.gov (United States)

    Rohani, Pejman; Najafi Sani, Mehri; Ahmadi, Mitra; Ziaee, Vahid

    2017-01-01

    Background. Eosinophilic esophagitis is an inflammatory condition where there is a dense infiltration of eosinophils typically exceeding fifteen cells per high power field. Familial Mediterranean fever is an autosomal recessive disorder characterized by brief, acute, and self-limited episodes of fever and polyserositis that recur at irregular intervals. Case Presentation. A three-year-and-nine-month-old Iranian girl was admitted to our center. The patient's parents complained of a history of abdominal pain, poor appetite, and poor weight gain from 1.5 years ago and episodes of food impaction after starting solid foods. Eosinophilic esophagitis was diagnosed based on histology. Because of continuing abdominal pain after treatment of eosinophilic esophagitis, the episodic nature of disease, and the presence of fever with pain, screening for familial Mediterranean fever mutation was performed and the patient was found to be heterozygote for Mediterranean fever. Conclusion. We have reported a case of eosinophilic esophagitis coexisting with familial Mediterranean fever which has not been described previously.

  17. A Case of Eosinophilic Esophagitis Accompanying Familial Mediterranean Fever

    Science.gov (United States)

    Rohani, Pejman; Najafi Sani, Mehri; Ahmadi, Mitra

    2017-01-01

    Background. Eosinophilic esophagitis is an inflammatory condition where there is a dense infiltration of eosinophils typically exceeding fifteen cells per high power field. Familial Mediterranean fever is an autosomal recessive disorder characterized by brief, acute, and self-limited episodes of fever and polyserositis that recur at irregular intervals. Case Presentation. A three-year-and-nine-month-old Iranian girl was admitted to our center. The patient's parents complained of a history of abdominal pain, poor appetite, and poor weight gain from 1.5 years ago and episodes of food impaction after starting solid foods. Eosinophilic esophagitis was diagnosed based on histology. Because of continuing abdominal pain after treatment of eosinophilic esophagitis, the episodic nature of disease, and the presence of fever with pain, screening for familial Mediterranean fever mutation was performed and the patient was found to be heterozygote for Mediterranean fever. Conclusion. We have reported a case of eosinophilic esophagitis coexisting with familial Mediterranean fever which has not been described previously. PMID:28255474

  18. Unusual Paraneoplastic Syndrome Accompanies Neuroendocrine Tumours of the Pancreas

    Directory of Open Access Journals (Sweden)

    Helga Bertani

    2011-01-01

    Full Text Available Neuroendocrine tumours comprise a small percentage of pancreatic neoplasia (10% (1. Diagnosis of neuroendocrine tumours is difficult, especially if the tumours are small and nonfunctional. CT scans, MRI, and nuclear scans are sufficiently sensitive assessment tools for tumours with diameters of at least 2 cm; otherwise, the sensitivity and specificity of these techniques is less than 50% (2. Myasthenia gravis (MG is a heterogeneous neuromuscular junction disorder that is primarily caused when antibodies form against the acetylcholine receptors (Ab-AchR. MG can develop in conjunction with neoplasia, making MG a paraneoplastic disease. In those cases, MG is most commonly associated with thymomas and less frequently associated with extrathymic malignancies. The mechanism underlying this paraneoplastic syndrome has been hypothesized to involve an autoimmune response against the tumour cells (3. No published reports have linked malignant pancreatic diseases with MG. Here, we report the case of a young woman, negative for Ab-AchR, with a neuroendocrine tumour in the pancreatic head, who experienced a complete resolution of her MG-like syndrome after surgical enucleation of the tumour.

  19. Sacculo-collic pathway dysfunction accompanying auditory neuropathy.

    Science.gov (United States)

    Sheykholeslami, Kianoush; Schmerber, Sébastien; Habiby Kermany, Mohammad; Kaga, Kimitaka

    2005-07-01

    In a patient with bilateral auditory neuropathy (AN), the vestibular-evoked myogenic potential (VEMP) was probably absent because of a neuropathy involving the inferior vestibular nerve and/or its end organ, the saccule. Our result can therefore be interpreted as a concomitant unilateral sacculo-collic neuropathy. We suggest the use of more precise terms to characterize AN patients with involvement of different parts of the inner ear and its innervations. We encourage detailed vestibular assessment in patients with AN in order to assess the co-existence of any symptomatic or asymptomatic vestibular disorder. Information such as that provided in this report will be valuable for clinicians caring for this group of patients. AN is a disorder characterized by the absence or severe impairment of auditory brainstem responses in the presence of normal cochlear outer hair cell function as revealed by otoacoustic emissions (OAEs) and/or electrocochleography (ECoG). A variety of processes and etiologies are thought to be involved in its pathophysiology. In most literature reports the auditory profile of patients with AN is discussed. However, the extent of vestibular involvement, especially that involving the saccule, is not known. We performed vestibular tests to assess the status of the saccule in a patient with AN. One patient with AN was studied. The patient was a right-handed 21-year-old female with chief complaints of hearing loss and speech perception difficulty. The auditory test results were consistent with the diagnosis of AN, i.e. absent auditory brainstem responses, moderate hearing loss, an inappropriately profound speech discrimination score and the presence of OAEs and measurable cochlear microphonics on ECoG. On neurological examination, gait and balance tests were normal. Ice-water caloric testing induced a sensation of dizziness in both ears. Short tone-burst VEMPs showed no response on left-ear stimulation and a biphasic response with normal latency and

  20. 22 CFR 40.102 - Guardian required to accompany excluded alien.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Guardian required to accompany excluded alien. 40.102 Section 40.102 Foreign Relations DEPARTMENT OF STATE VISAS REGULATIONS PERTAINING TO BOTH... Guardian required to accompany excluded alien. INA 212(a)(9)(B) is not applicable at the time of visa...

  1. Study on feasibility of laser reflective tomography with satellite-accompany

    Science.gov (United States)

    Gu, Yu; Hu, Yi-hua; Hao, Shi-qi; Gu, You-lin; Zhao, Nan-xiang; Wang, Yang-yang

    2015-10-01

    Laser reflective tomography is a long-range, high-resolution active detection technology, whose advantage is that the spatial resolution is unrelated with the imaging distance. Accompany satellite is a specific satellite around the target spacecraft with encircling movement. When using the accompany satellite to detect the target aircraft, multi-angle echo data can be obtained with the application of reflective tomography imaging. The feasibility of such detection working mode was studied in this article. Accompany orbit model was established with horizontal circular fleet and the parameters of accompany flight was defined. The simulation of satellite-to-satellite reflective tomography imaging with satellite-accompany was carried out. The operating mode of reflective tomographic data acquisition from monostatic laser radar was discussed and designed. The flight period, which equals to the all direction received data consuming time, is one of the important accompany flight parameters. The azimuth angle determines the plane of image formation while the elevation angle determines the projection direction. Both of the azimuth and elevation angles guide the satellite attitude stability controller in order to point the laser radar spot on the target. The influences of distance between accompany satellite and target satellite on tomographic imaging consuming time was analyzed. The influences of flight period, azimuth angle and elevation angle on tomographic imaging were analyzed as well. Simulation results showed that the satellite-accompany laser reflective tomography is a feasible and effective method to the satellite-to-satellite detection.

  2. An extension of the Noether theorem: Accompanying equations possessing conservation laws

    Science.gov (United States)

    Dorodnitsyn, V. A.; Ibragimov, N. H.

    2014-02-01

    It is shown that the Noether theorem can be extended for some equations associated (accompanying) with Euler-Lagrange equation. Each symmetry of Lagrangian yields a class of accompanying equations possessing conservation law (first integral). The generalization is done for canonical Hamiltonian equations as well.

  3. 30 CFR 250.246 - What mineral resource conservation information must accompany the DPP or DOCD?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What mineral resource conservation information... Coordination Documents (docd) § 250.246 What mineral resource conservation information must accompany the DPP or DOCD? The following mineral resource conservation information, as applicable, must accompany...

  4. 39 CFR 320.7 - Suspension for advertisements accompanying parcels or periodicals.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Suspension for advertisements accompanying parcels... advertisements accompanying parcels or periodicals. (a) The operation of 39 U.S.C. 601(a) (1) through (6) and § 310.2(b) (1) through (6) of this chapter is suspended on all post routes for advertisements...

  5. 76 FR 44926 - Renewal of Declaration Regarding Emergency Use of Doxycycline Hyclate Tablets Accompanied by...

    Science.gov (United States)

    2011-07-27

    ... Declaration Regarding Emergency Use of Doxycycline Hyclate Tablets Accompanied by Emergency Use Information and Amendment To Include All Oral Formulations of Doxycycline AGENCY: Office of the Secretary (OS... authorization of emergency use of doxycycline hyclate tablets accompanied by emergency use information...

  6. 30 CFR 250.215 - What hydrogen sulfide (H2S) information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What hydrogen sulfide (H2S) information must... Contents of Exploration Plans (ep) § 250.215 What hydrogen sulfide (H2S) information must accompany the EP? The following H2S information, as applicable, must accompany your EP: (a) Concentration. The estimated...

  7. 7 CFR 319.37-12 - Prohibited articles accompanying restricted articles.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Prohibited articles accompanying restricted articles... Stock, Plants, Roots, Bulbs, Seeds, and Other Plant Products 1,2 § 319.37-12 Prohibited articles accompanying restricted articles. A restricted article for importation into the United States shall not...

  8. Values of Seven Tumor Markers in Identiifcation and Diagnosis of Esophageal Carcinoma Accompanied by Neuroendocrine Differentiation

    Institute of Scientific and Technical Information of China (English)

    LIU Lian-ke; SHAO Ming-wen; MA Lan; SUN Jing; GUAN Dan; SHU Yongqian

    2015-01-01

    Objective:To explore the values of seven tumor markers in the identiifcation and diagnosis of esophageal carcinoma accompanied by neuroendocrine differentiation (E-NED). Methods:A total of 378 patients diagnosed as low differentiation of esophageal carcinoma in The First Affiliated Hospital with Nanjing Medical University from Jan., 2008 to Dec., 2013 were selected, in which there were 349 with esophageal carcinoma with no neuroendocrine differentiation (E-NNED, E-NNED group) and 29 with E-NED (E-NED group). The levels of seven tumor markers including synaptophysin (Syn), Chromogranin A (CgA), neuron-specific enolase (NSE), neural cell adhesion molecule (CD56), protein gene product 9.5 (PGP9.5), secretagogue (SCGN) and thyroid transcription factor-1 (TTF-1) of both groups were detected with histoimmunochemical method and the influences of the single and combined detection of above indexes on E-NED patients were analyzed. Results:Except TTF-1, expressions of Syn, CgA, NSE, CD56, PGP9.5 and SCGN in E-NED group were evidently higher than those in E-NNED group and the differences were significant (P Conclusion:PGP9.5 and SCGN can be used as neuroendocrine markers for the pathological diagnosis of E-NED and Syn + CD56, Syn + PGP9.5 and Syn + SCGN can all be used as combined detection.

  9. Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis.

    Science.gov (United States)

    Hoque, Tahsina Sharmin; Uraji, Misugi; Ye, Wenxiu; Hossain, Mohammad Anowar; Nakamura, Yoshimasa; Murata, Yoshiyuki

    2012-07-01

    Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive α-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca²⁺](cyt)) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca²⁺](cyt) oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca²⁺](cyt) oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca²⁺](cyt) oscillations. Copyright © 2012 Elsevier GmbH. All rights reserved.

  10. Homology analysis detects topological changes of Iba1 localization accompanied by microglial activation.

    Science.gov (United States)

    Sawano, Toshinori; Tsuchihashi, Ryo; Morii, Eiichi; Watanabe, Fumiya; Nakane, Kazuaki; Inagaki, Shinobu

    2017-03-27

    The state of microglial activation provides important information about the central nervous system. However, a reliable index of microglial activation in histological samples has yet to be established. Here, we show that microglial activation induces topological changes of Iba1 localization that can be detected by analysis based on homology theory. Analysis of homology was applied to images of Iba1-stained tissue sections, and the 0-dimentional Betti number (b0: the number of solid components) and the 1-dimentional Betti number (b1: the number of windows surrounded by solid components) were obtained. We defined b1/b0 as the Homology Value (HV), and investigated its validity as an index of microglial activation using cerebral ischemia model mice. Microglial activation was accompanied by changes to Iba1 localization and morphology of microglial processes. In single microglial cells, the change of Iba1 localization increased b1. Conversely, thickening or retraction of microglial processes decreased b0. Consequently, microglial activation increased the HV. The HV of a tissue area increased with proximity to the ischemic core and showed a high degree of concordance with the number of microglia expressing activation makers. Furthermore, the HV of human metastatic brain tumor tissue also increased with proximity to the tumor. These results suggest that our index, based on homology theory, can be used to correctly evaluate microglial activation in various tissue images. Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

  11. Monitoring of the Enzymatic Degradation of Protein Corona and Evaluating the Accompanying Cytotoxicity of Nanoparticles.

    Science.gov (United States)

    Ma, Zhifang; Bai, Jing; Jiang, Xiue

    2015-08-19

    Established nanobio interactions face the challenge that the formation of nanoparticle-protein corona complexes shields the inherent properties of the nanoparticles and alters the manner of the interactions between nanoparticles and biological systems. Therefore, many studies have focused on protein corona-mediated nanoparticle binding, internalization, and intracellular transportation. However, there are a few studies to pay attention to if the corona encounters degradation after internalization and how the degradation of the protein corona affects cytotoxicity. To fill this gap, we prepared three types of off/on complexes based on gold nanoparticles (Au NPs) and dye-labeled serum proteins and studied the extracellular and intracellular proteolytic processes of protein coronas as well as their accompanying effects on cytotoxicity through multiple evaluation mechanisms, including cell viability, adenosine triphosphate (ATP) content, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS). The proteolytic process was confirmed by recovery of the fluorescence of the dye-labeled protein molecules that was initially quenched by Au NPs. Our results indicate that the degradation rate of protein corona is dependent on the type of the protein based on systematical evaluation of the extracellular and intracellular degradation processes of the protein coronas formed by human serum albumin (HSA), γ-globulin (HGG), and serum fibrinogen (HSF). Degradation is the fastest for HSA corona and the slowest for HSF corona. Notably, we also find that the Au NP-HSA corona complex induces lower cell viability, slower ATP production, lower MMP, and higher ROS levels. The cytotoxicity of the nanoparticle-protein corona complex may be associated with the protein corona degradation process. All of these results will enrich the database of cytotoxicity induced by nanomaterial-protein corona complexes.

  12. Vascular Injury Accompanying Displaced Proximal Humeral Fractures: Two Cases and a Review of the Literature

    Directory of Open Access Journals (Sweden)

    Martijn Hofman

    2011-01-01

    Full Text Available We present two cases in which displaced proximal humeral fractures are accompanied by vascular injury. These Injuries are very rare but severe and the accompanying vascular impairment can have great clinical consequences. Therefore, we try to emphasize on the importance of thorough and accurate diagnostics, because it is obligatory for early diagnosis and improving the eventual outcome of these injuries. The specific order in treatment (internal fixation first or vascular repair first depends on the severity of the accompanying vascular injury. The increasing use of endovascular procedures to treat vascular lesions is a very interesting development with several advantages, especially in elderly and multimorbid patients.

  13. Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles.

    Science.gov (United States)

    Sinturel, Flore; Gerber, Alan; Mauvoisin, Daniel; Wang, Jingkui; Gatfield, David; Stubblefield, Jeremy J; Green, Carla B; Gachon, Frédéric; Schibler, Ueli

    2017-05-04

    The liver plays a pivotal role in metabolism and xenobiotic detoxification, processes that must be particularly efficient when animals are active and feed. A major question is how the liver adapts to these diurnal changes in physiology. Here, we show that, in mice, liver mass, hepatocyte size, and protein levels follow a daily rhythm, whose amplitude depends on both feeding-fasting and light-dark cycles. Correlative evidence suggests that the daily oscillation in global protein accumulation depends on a similar fluctuation in ribosome number. Whereas rRNA genes are transcribed at similar rates throughout the day, some newly synthesized rRNAs are polyadenylated and degraded in the nucleus in a robustly diurnal fashion with a phase opposite to that of ribosomal protein synthesis. Based on studies with cultured fibroblasts, we propose that rRNAs not packaged into complete ribosomal subunits are polyadenylated by the poly(A) polymerase PAPD5 and degraded by the nuclear exosome. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Christoph Rothmann's discourse on the comet of 1585 an edition and translation with accompanying essays

    CERN Document Server

    Granada, Miguel A; Jardine, Nicholas

    2014-01-01

    Christoph Rothmann's Discourse on the Comet of 1585 offers the first edition of the Latin treatise after it was published in 1619. It is accompanied by an English translation and a full introduction and commentary.

  15. [Letter and accompanying Bill proposing the creation of the Agassiz Wilderness

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This reference contains both the letter, addressed to the Speaker of the House, proposing the creation of thirty-seven new wilderness areas, and the accompanying...

  16. [Introduction of accompanying documents on the new EMC standard of medical electrical equipment].

    Science.gov (United States)

    Wang, Weiming

    2013-07-01

    The article introduces the requirements of accompanying documents on the new EMC standard of medical electrical equipment (YY 0505-2012), hope it can be useful for the manufacturers of medical electrical equipment.

  17. HEREDITARY DISEASES AND SYNDROMES ACCOMPANIED BY FEBRILE CONVULSIONS: CLINICAL AND GENETIC CHARACTERISTICS AND DIAGNOSTIC PROCEDURES

    National Research Council Canada - National Science Library

    E. L. Dadali; A. A. Sharkov; I. V. Sharkova; I. V. Kanivets; F. A. Konovalov; I. A. Akimova

    2016-01-01

    The authors provide a review of the clinical and genetic characteristics of hereditary diseases and syndromes accompanied by febrile convulsions, which is illustrated by examples of their own observations...

  18. Results of dental implantation at patients with the accompanying somatic pathology

    OpenAIRE

    2016-01-01

    Until recently operations of dental implantation were performed at patients of not having associated diseases. Now researches of opportunities of dental implantation at different types of the accompanying somatopathies are conducted.

  19. Red Ear and More: Facial and Extrafacial Erythema Accompanying Migraine Attacks.

    Science.gov (United States)

    Velasco, Elena Martínez; Mesonero, Luis López; Hueso, María Isabel Pedraza; Piñero, Marina Ruiz; de Lera Alfonso, Mercedes; Peral, Ángel Luis Guerrero

    2016-01-01

    Cutaneous manifestations of migraine are infrequent and their spectrum is reduced to the red ear syndrome (RES) and eyelid disorders. We report a case of a 26-year-old woman with migraine accompanied by extensive erythema, which involved right ear and cheek and left hemithorax. She fulfilled proposed criteria of RES. We started preventive therapy with a significant response. This is the first description in the literature of an erythema accompanying migraine attacks broadly exceeding the ear.

  20. A Rare Entity: Bilateral First Rib Fractures Accompanying Bilateral Scapular Fractures

    Directory of Open Access Journals (Sweden)

    Gultekin Gulbahar

    2015-01-01

    Full Text Available First rib fractures are scarce due to their well-protected anatomic locations. Bilateral first rib fractures accompanying bilateral scapular fractures are very rare, although they may be together with scapular and clavicular fractures. According to our knowledge, no case of bilateral first rib fractures accompanying bilateral scapular fractures has been reported, so we herein discussed the diagnosis, treatment, and complications of bone fractures due to thoracic trauma in bias of this rare entity.

  1. Refractory ulcerative colitis accompanied with cytomegalovirus colitis and multiple liver abscesses:A case report

    Institute of Scientific and Technical Information of China (English)

    Takuya Inoue; Ken Toshina; Takashi Nishikawa; Norihiro Hamamoto; Ken Nakagawa; Ken-Ichi Katsu; Ichiro Hirata; Yutaro Egashira; Kumi Ishida; Ken Kawakami; Eijiro Morita; Naoko Murano; Shingo Yasumoto; Mitsuyuki Murano

    2005-01-01

    Various hepato-biliary complications are an increased incidence in patients with inflammatory bowel disease,and portal bacteremia is well documented in patients with ulcerative colitis (UC). However, few reports mention UC in association with liver abscesses. Recently, there are several reports describing cytomegalovirus (CMV) infection in association with disease exacerbation and steroid refractoriness in patients with UC. Here we present a case of refractory UC accompanied with multiple liver abscesses and CMV colitis. The patient, a 72-year-old male, with a five-year history of repeated admissions to our hospital for UC, presented with an exacerbation of his UC.Sigmoidoscopy performed on admission suggested that his UC was exacerbated, then he was given prednisolone and mesalazine orally, and betamethasone enemas.However, he had exacerbated symptoms. Repeat sigmoidoscopy revealed multiple longitudinal ulcers and pseudopolyps in the rectosigmoid colon. Although immunohistochemical staining of biopsy specimens and the serum testing for antigenemia were negative on admission and after the repeat sigmoidoscopy, they became histologically positive for CMV. Nonetheless, the patient developed spiking fevers, soon after ganciclovir was administered. Laboratory studies revealed an increased white cell count with left shift, and Enterococcus fecalis grew in blood cultures. An abdominal computed tomography (CT) scan was obtained and the diagnosis of liver abscesses associated with UC was made, based on CT results. The hepatic abscesses were successfully treated with intravenous meropenem for 6 wk, without further percutaneous drainage. To our knowledge, this is the first reported case of multiple liver abscesses that develop during UC exacerbation complicated by CMV colitis.

  2. Kimura's disease accompanied by mediastinal lymphadenopathy misdiagnosed as lung cancer: Case report and review of literatures

    Directory of Open Access Journals (Sweden)

    Xi SUN

    2012-06-01

    Full Text Available Objective  To further investigate the diagnostic pitfalls of Kimura's disease(KD. Methods  We report one case of Kimura's disease accompanied with mediastinal lymphadenopathy admitted to General Hospital of Chinese PLA in the year 2010. After searching on PubMed and Chinese Medical Journal Searching Engines(Wanfang data base,Cqvip data base,we have found literatures reporting another 3 cases of Kimura's disease with mediastinal lymphadenopathy. Plus the one we presented, all four patients' clinical data were collected and analyzed thereafter. Up-to-date advancement on KD's diagnosis and therapy would also be reviewed. Results  Our case, a 61-year-old male, was initially misdiagnosed as lung cancer with mediastinal metastasis in 2008, but histopathology had confirmed only eosinophil infiltration in enlarged mediasinal lymph nodes, no malignant cells were identified. In the year 2010, he complained bilateral orbital masses, one of which was surgically removed, and was later confirmed as Kimura's disease by histopathology. Clinical features of 4 patients, including our case and the other 3 retrieved in literatures, were available, 2 of whom were misdiagnosed as lung cancer while the other 2 as lymphoma in the beginning. All the 4 patients were responsive to steroid therapy. Conclusions  Kimura's disease could be associated with mediastinal lymphadenopathy which may confuse the physicians in diagnosing. Newly developed minimal invasive approaches such as endoscopic ultrasound-guided fine needle aspiration (EUS-FNA and endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA are helpful for the differential diagnosis.

  3. Increased vascular density and vitreo-retinal membranes accompany vascularization of the pigment epithelium in the dystrophic rat retina.

    Science.gov (United States)

    Caldwell, R B; Roque, R S; Solomon, S W

    1989-09-01

    Observations of vascularization of the retinal pigment epithelium (RPE) and formation of vitreo-retinal membranes (VRMs) in Royal College of Surgeons (RCS) rats with inherited retinal dystrophy suggest that vascular proliferation occurs in this model. To test this hypothesis, we studied the progression of vascular changes in RCS and age-matched control rats using quantitative light microscope morphometry and electron microscopy. At 2 weeks, prior to photoreceptor degeneration, the dystrophic retina is comparable with the control. By 2 months, extensive degeneration of photoreceptor cells results in significant thinning of the dystrophic retina as compared with the control. Signs of vascular degeneration are evident at the electron microscope level--"ghost" vessels consisting of acellular basal lamina surrounded by amorphous electron-dense material; degenerating endothelial cells and pericytes; and abnormal deposits of extracellular matrix (ECM) material around blood vessels. Vascular degeneration is accompanied by glial changes in the form of necrotic perivascular glial processes and abnormal ECM deposits among the altered Muller cell processes. At 2-4 months in the dystrophic retina, numbers of vessel profiles in dystrophic retinas are decreased as compared with controls. However, vascular degeneration is overshadowed by the formation of numerous capillary tufts within the RPE layer, which together with retinal thinning results in increased vessel density. Between 4-12 months, the retinal thickness diminishes further, vascularization of the RPE increases, vitreo-retinal membranes are formed, and vascular density increases. In summary, following an initial period of vascular degeneration, vascularization of the RPE is accompanied by an increase in retinal vessel density and by the formation of vitreo-retinal membranes.

  4. Knowledge and expectations of the accompanying member concerning illnesses and hospitalization of the newborn

    Directory of Open Access Journals (Sweden)

    Maria da Gloria Machado de Oliveira

    2015-02-01

    Full Text Available This study aimed at describing the knowledge, difficulties and expectations of accompanying members concerning illnesses and hospitalization of the newborn. It is a qualitative study with 11 accompanying members of infants admitted in public hospital, in Fortaleza, CE, Brazil. It consists of semi-structured interviews carried out and recorded in June 2012. It was based on the content analysis that emerged from three categories: knowing the health problems of the newborn, expectations in the hospital context and difficulties faced by accompanying members. It was observed that the accompanying members had limited knowledge about the health conditions that resulted in the hospitalization of the newborn, although there was a professional health team for the guidelines, for the affected needs. Commonly speaking, it expresses limited information, but possible to be understood. It is essential that the health professionals provide to the accompanying members on the disease, treatment and recovery of the newborn so that they can understand the process of hospitalization and provide proper assistance.

  5. Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.

    Science.gov (United States)

    Thakur, Anita; Bhatla, Satish C

    2015-01-01

    A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20-30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20-30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes. These include proteins involved in energy metabolism, reactive oxygen scavenging, proteolysis and protein turnover, signaling, oleosin and oil body biogenesis-associated proteins, desiccation and cytoskeleton. At 30 DAA stage, exclusive expressions of enzymes belonging to energy metabolism, desiccation and cytoskeleton were evident which indicated an increase in the metabolic and enzymatic activity in the cells at this stage of seed development (seed filling). Increased expression of cruciferina-like protein and dehydrin at 30 DAA stage marks the onset of desiccation. The data has been analyzed and discussed to highlight desiccation stage-associated metabolic events during oilseed development.

  6. The Evaluation of Adrenal Function in Two Cases of Hypocortisolism Accompanied by Liver Cirrhosis.

    Science.gov (United States)

    Yamashita, Maki; Kageyama, Kazunori; Murakami, Hiroshi; Sugiyama, Aya; Yanagimachi, Miyuki; Sato, Eri; Murasawa, Shingo; Matsui, Jun; Tamasawa, Naoki; Daimon, Makoto

    2016-01-01

    Adrenal insufficiency may occur in patients with liver cirrhosis. The assessment of hypothalamus-pituitary-adrenal function is important in such patients, but there is no consensus as to how it should be performed. We herein report the results of our evaluation of the adrenal function in two patients with hypocortisolism accompanied by liver cirrhosis. The patients lacked the typical features of hypocortisolism. One was diagnosed with hypocortisolism accompanied by liver cirrhosis while the other had secondary adrenal insufficiency caused by a hypothalamic disorder. Hypocortisolism accompanied by liver cirrhosis should be evaluated by endocrine tests to determine its pathogenesis. A low-dose adrenocorticotropic hormone test may be appropriate for non-critically ill cirrhotic patients.

  7. 78 FR 66357 - Proposed Changes to FCC Form 499-A, FCC Form 499-Q, and Accompanying Instructions

    Science.gov (United States)

    2013-11-05

    ... COMMISSION Proposed Changes to FCC Form 499-A, FCC Form 499-Q, and Accompanying Instructions AGENCY: Federal... annual Telecommunications Reporting Worksheet, FCC Form 499-A (Form 499-A) and accompanying instructions... Telecommunications Reporting Worksheet, FCC Form 499-Q (Form 499-Q) and accompanying instructions (Form...

  8. Steam thermolysis of tire shreds: modernization in afterburning of accompanying gas with waste steam

    Science.gov (United States)

    Kalitko, V. A.

    2010-03-01

    On the basis of experience in the commercial operation of tire-shred steam thermolysis in EnresTec Inc. (Taiwan) producing high-grade commercial carbon, liquid pyrolysis fuel, and accompanying fuel gas by this method, we have proposed a number of engineering solutions and calculated-analytical substantiations for modernization and intensification of the process by afterburning the accompanying gas with waste steam condensable in the scrubber of water gas cleaning of afterburning products. The condensate is completely freed of the organic pyrolysis impurities and the necessity of separating it from the liquid fuel, as is the case with the active process, is excluded.

  9. Totally laparoscopic gastrectomy for early gastric cancer accompanied by huge hiatal hernia: A case report.

    Science.gov (United States)

    Hagiwara, Chie; Yajima, Kazuhito; Iwasaki, Yoshiaki; Oohinata, Ryouki; Yuu, Ken; Ishiyama, Satoshi; Amaki, Misato; Nakano, Daisuke; Yamaguchi, Tatsuro; Matsumoto, Hiroshi; Takahashi, Keiichi

    2016-02-01

    We herein present a case in which we used a totally laparoscopic approach for early gastric cancer accompanied by a huge hiatal hernia. An 80-year-old Japanese woman was referred with a chief complaint of dysphagia. A clinical diagnosis of early gastric cancer, T1b (SM) N0M0, stage IA, accompanied by hiatal hernia, was made. Distal gastrectomy with D1 plus lymphadenectomy was carried out. After the gastrectomy, the hernial sac was excised and the hernial orifice was closed. Reconstruction using the Roux-en-Y method was selected. The postoperative course was uneventful and she was discharged on postoperative day 10.

  10. Training shortens search times in children with visual impairment accompanied by nystagmus

    NARCIS (Netherlands)

    Huurneman, B.; Boonstra, F.N.

    2014-01-01

    Perceptual learning (PL) can improve near visual acuity (NVA) in 4-9 year old children with visual impairment (VI). However, the mechanisms underlying improved NVA are unknown. The present study compares feature search and oculomotor measures in 4-9 year old children with VI accompanied by nystagmus

  11. [Clinical features of sudden sensorineural hearing loss accompanied with inner ear hemorrhage].

    Science.gov (United States)

    Chen, Xihang; Lin, Chang; Fang, Zheming; Chen, Xi; Ye, Shengnan; Cheng, Jinmei; Zhang, Rong

    2015-12-01

    To investigate the clinical features, diagnosis and prognosis of sudden sensorineural hearing loss accompanied with inner ear hemorrhage. Eleven cases of sudden sensorineural hearing loss accompanied with inner ear hemorrhage were retrospectively analyzed, including clinical manifestation, hematological and audiological examinations, as well as characteristics of inner ear three-dimensional fluid-attenuated inversion recovery magnetic resonance imaging (3D-FLAIR MRI). Eleven cases of sudden hearing loss with inner ear hemorrhage were accompanied by varying degrees of vertigo, lasting from several minutes to several hours, nine of whom had persistent tinnitus. The audiometry curves of the 11 cases included total deafness or flat descending type. The inner ear 3D-FLAIR MRI studies revealed abnormal high signals at different positions of the inner ear, one case with endolymphatic hydrops. After the treatment, seven cases were invalid, two cases had notable effect, and two cases were effective. Abnormal high signals in the inner ear were sensitively detected by 3D-FLAIR MRI sequence, which was applied for the diagnosis of the sudden hearing loss accompanied with inner ear hemorrhage. The effect of conventional treatment was not good and the appropriate treatments for this type of sudden hearing loss need further investigation.

  12. 41 CFR 101-25.110-3 - Tires accompanying new motor vehicles.

    Science.gov (United States)

    2010-07-01

    ... motor vehicles. 101-25.110-3 Section 101-25.110-3 Public Contracts and Property Management Federal...-GENERAL 25.1-General Policies § 101-25.110-3 Tires accompanying new motor vehicles. The tire identifications and recordkeeping regulations issued by the Department of Transportation require each...

  13. UNIQUENESS OF LIMIT CYCLE FOR A CLASS OF QUARTIC SYSTEM ACCOMPANYING WITH QUADRATIC SYSTEM

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In this paper,we consider a class of quartic system,which is more general and realistic than the quartic accompanying system. Consequently,we obtain sufficient conditions concerning the uniqueness of limit cycle as well as some other in-depth conclusions.

  14. 30 CFR 250.226 - What Coastal Zone Management Act (CZMA) information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What Coastal Zone Management Act (CZMA) information must accompany the EP? 250.226 Section 250.226 Mineral Resources MINERALS MANAGEMENT SERVICE... and Information Contents of Exploration Plans (ep) § 250.226 What Coastal Zone Management Act...

  15. Clinical accompaniment: the critical care nursing students’ experiences in a private hospital

    Directory of Open Access Journals (Sweden)

    N. Tsele

    2000-09-01

    Full Text Available The quality of clinical accompaniment of the student enrolled for the post-basic diploma in Medical and Surgical Nursing Science: Critical Care Nursing (General is an important dimension of the educational/learning programme. The clinical accompanist/mentor is responsible for ensuring the student’s compliance with the clinical outcomes of the programme in accordance with the requirements laid down by the Nursing Education Institution and the South African Nursing Council. The purpose of this study was to explore and describe the experiences of the students enrolled for a post-basic diploma in Medical and Surgical Nursing Science: Critical Care Nursing (General, in relation to the clinical accompaniment in a private hospital in Gauteng. An exploratory, descriptive and phenomenological research design was utilised and individual interviews were conducted with the ten students in the research hospital. A content analysis was conducted and the results revealed both positive and negative experiences by the students in the internal and external worlds. The recommendations include the formulation of standards for clinical accompaniment of students. the evaluation of the quality of clinical accompaniment of students and empowerment of the organisation, clinical accompanists/mentors and clinicians.

  16. STUDY OF THE GAMMA EMISSION PROBABILITY ACCOMPANYING THE SPONTANEOUS FISSION OF CF-252

    NARCIS (Netherlands)

    VANDERPLOEG, H; LAURENS, CR; BACELAR, JCS; BUDA, A; GAARDHOJE, JJ; VANTHOF, G; KALANTARNAYESTANAKI, N; VANDERWOUDE, A; ZELAZNY, Z

    1994-01-01

    A study of the gamma emission accompanying the spontaneous fission process of Cf-252 has been performed. The photon emission probability between 3 and 70 MeV and its angular dependence with respect to the fission direction were measured. These measurements were performed as a function of the mass as

  17. Congenital nasal pyriform aperture stenosis: A case with accompanying short lingual frenulum

    Directory of Open Access Journals (Sweden)

    Ehsan Amini

    2016-01-01

    Full Text Available Congenital nasal pyriform aperture stenosis (CNPAS is a rare cause of nasal obstruction. We presented a case of CNPAS with accompanying short lingual frenulum. Surgical dilatation without osteotomy was used, and the infant had normal growth and development. In these cases, the less invasive surgical methods can be effective.

  18. 30 CFR 250.223 - What mitigation measures information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What mitigation measures information must... INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents of Exploration Plans (ep) § 250.223 What mitigation measures information must accompany the EP?...

  19. 30 CFR 250.254 - What mitigation measures information must accompany the DPP or DOCD?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What mitigation measures information must... Information Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents (docd) § 250.254 What mitigation measures information must accompany the DPP or DOCD? (a) If...

  20. 30 CFR 250.243 - What general information must accompany the DPP or DOCD?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What general information must accompany the DPP or DOCD? 250.243 Section 250.243 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE... Contents of Development and Production Plans (dpp) and Development Operations Coordination Documents...

  1. Analysis on the environmental problems accompanying the high-speed urbanization of small towns in China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The environmental problems accompanying the rapid urbanization of many small towns like Keqiao, SHaoxing County, and their countermeasures are presented. To settle these problems rationally as well as effectively will not only conduce to boost the environmental quality and life quality of cities, but also help to promote the development of regional economy and the realization of sustainable development.

  2. Analysis on the environmental problems accompanying the high-speed urbanization of small towns in China.

    Science.gov (United States)

    Xu, Lei; Ge, Jian; Hua, Ying

    2002-01-01

    The environmental problems accompanying the rapid urbanization of many small towns like Keqiao, Shaoxing County of China and their countermeasures are presented. To settle these problems rationally as well as effectively will not only conduce to boost the environmental quality and life quality of cities, but also help to promote the development of regional economy and the realization of sustainable development.

  3. Measurement of isolated photons accompanied by jets in deep inelastic ep scattering

    NARCIS (Netherlands)

    Abramowicz, H.; Abt, I.; Adamczyk, L.; Adamus, M.; Aggarwal, R.; Antonelli, S.; Antonioli, P.; Antonov, A.; Arneodo, M.; Aushev, V.; Aushev, Y.; Bachynska, O.; Bamberger, A.; Barakbaev, A. N.; Barbagli, G.; Bari, G.; Barreiro, F.; Bartosik, N.; Bartsch, D.; Basile, M.; Behnke, O.; Behr, J.; Behrens, U.; Bellagamba, L.; Bertolin, A.; Bhadra, S.; Bindi, M.; Blohm, C.; Bokhonov, V.; Bold, T.; Bondarenko, K.; Boos, E. G.; Borras, K.; Boscherini, D.; Brock, I.; Brownson, E.; Brugnera, R.; Bruemmer, N.; Bruni, A.; Bruni, G.; Brzozowska, B.; Bussey, P. J.; Bylsma, B.; Caldwell, A.; Capua, M.; Carlin, R.; Catterall, C. D.; Chekanov, S.; Chwastowski, J.; Ciborowski, J.; Ciesielski, R.; Cifarelli, L.; Cindolo, F.; Contin, A.; Cooper-Sarkar, A. M.; Coppola, N.; Corradi, M.; Corriveau, F.; Costa, M.; D'Agostini, G.; Dal Corso, F.; del Peso, J.; Dementiev, R. K.; De Pasquale, S.; Derrick, M.; Devenish, R. C. E.; Dobur, D.; Dolgoshein, B. A.; Dolinska, G.; Doyle, A. T.; Drugakov, V.; Durkin, L. S.; Dusini, S.; Eisenberg, Y.; Ermolov, P. F.; Eskreys, A.; Fazio, S.; Ferrando, J.; Ferrero, M. I.; Figiel, J.; Forrest, M.; Foster, B.; Gach, G.; Galas, A.; Gallo, E.; Garfagnini, A.; Geiser, A.; Gialas, I.; Gizhko, A.; Gladilin, L. K.; Gladkov, D.; Glasman, C.; Gogota, O.; Golubkov, Yu. A.; Goettlicher, P.; Grabowska-Bold, I.; Grebenyuk, J.; Gregor, I.; Grigorescu, G.; Grzelak, G.; Gueta, O.; Guzik, M.; Gwenlan, C.; Haas, T.; Hain, W.; Hamatsu, R.; Hart, J. C.; Hartmann, H.; Hartner, G.; Hilger, E.; Hochman, D.; Hori, R.; Huettmann, A.; Ibrahim, Z. A.; Iga, Y.; Ingbir, R.; Ishitsuka, M.; Jakob, H. -P.; Januschek, F.; Jones, T. W.; Juengst, M.; Kadenko, I.; Kahle, B.; Kananov, S.; Kanno, T.; Karshon, U.; Karstens, F.; Katkov, I. I.; Kaur, M.; Kaur, P.; Keramidas, A.; Khein, L. A.; Kim, J. Y.; Kisielewska, D.; Kitamura, S.; Klanner, R.; Klein, U.; Kondrashova, N.; Kononenko, O.; Kooijman, P.; Korol, Ie; Korzhavina, I. A.; Kotanski, A.; Koetz, U.; Kowalski, H.; Kuprash, O.; Kuze, M.; Lee, A.; Levchenko, B. B.; Libov, V.; Limentani, S.; Ling, T. Y.; Lisovyi, M.; Lobodzinska, E.; Lohmann, W.; Loehr, B.; Lohrmann, E.; Long, K. R.; Longhin, A.; Lontkovskyi, D.; Lukina, O. Yu; Maeda, J.; Magill, S.; Makarenko, I.; Malka, J.; Mankel, R.; Margotti, A.; Marini, G.; Mastroberardino, A.; Mattingly, M. C. K.; Melzer-Pellmann, I. -A.; Mergelmeyer, S.; Miglioranzi, S.; Idris, F. Mohamad; Monaco, V.; Montanari, A.; Mujkic, K.; Musgrave, B.; Nagano, K.; Namsoo, T.; Nania, R.; Nigro, A.; Ning, Y.; Nobe, T.; Notz, D.; Nowak, R. J.; Nuncio-Quiroz, A. E.; Oh, B. Y.; Okazaki, N.; Olkiewicz, K.; Onishchuk, Yu.; Papageorgiu, K.; Parenti, A.; Pawlak, J. M.; Pawlik, B.; Pelfer, P. G.; Pellegrino, A.; Perlanski, W.; Perrey, H.; Piotrzkowski, K.; Plucinski, P.; Pokrovskiy, N. S.; Polini, A.; Proskuryakov, A. S.; Przybycien, M.; Raval, A.; Reeder, D. D.; Reisert, B.; Ren, Z.; Repond, J.; Ri, Y. D.; Robertson, A.; Roloff, P.; Rubinsky, I.; Ruspa, M.; Sacchi, R.; Samson, U.; Sartorelli, G.; Savin, A. A.; Saxon, D. H.; Schioppa, M.; Schlenstedt, S.; Schleper, P.; Schmidke, W. B.; Schneekloth, U.; Schoenberg, V.; Schoerner-Sadenius, T.; Schwartz, J.; Sciulli, F.; Shcheglova, L. M.; Shehzadi, R.; Singh, I.; Skillicorn, I. O.; Slominski, W.; Smith, W. H.; Sola, V.; Solano, A.; Son, D.; Sosnovtsev, V.; Spiridonov, A.; Stadie, H.; Stanco, L.; Stefaniuk, N.; Stern, A.; Stewart, T. P.; Stifutkin, A.; Stopa, P.; Suchkov, S.; Susinno, G.; Suszycki, L.; Sztuk-Dambietz, J.; Szuba, D.; Szuba, J.; Tapper, A. D.; Tassi, E.; Terron, J.; Theedt, T.; Tiecke, H.; Tokushuku, K.; Tomaszewska, J.; Trusov, V.; Tsurugai, T.; Turcato, M.; Turkot, O.; Tymieniecka, T.; Vazquez, M.; Verbytskyi, A.; Viazlo, O.; Vlasov, N. N.; Walczak, R.; Abdullah, W. A. T. Wan; Whitmore, J. J.; Wichmann, K.; Wing, M.; Wlasenko, M.; Wolf, G.; Wolfe, H.; Wrona, K.; Yaguees-Molina, A. G.; Yamada, S.; Yamazaki, Y.; Yoshida, R.; Youngman, C.; Zabiegalov, O.; Zarnecki, A. F.; Zawiejski, L.; Zenaiev, O.; Zeuner, W.; Zhautykov, B. O.; Zhmak, N.; Zichichi, A.; Zolkapli, Z.; Zotkin, D. S.

    2012-01-01

    The production of isolated high-energy photons accompanied by jets has been measured in deep inelastic ep scattering with the ZEUS detector at HERA, using an integrated luminosity of 326 pb(-1). Measurements were made for exchanged photon virtualities Q(2), in the range 10 to 350 GeV2. The photons w

  4. Prepositions in Use: Prepositions of Standard, Prepositions of Possession and Prepositions of Accompaniment

    Science.gov (United States)

    AlQbailat, Naji Masned; Al-Momani, Islam M.; Almahameed, Yazan Shaker

    2016-01-01

    The current research paper attempted at investigating the use of prepositions of standard, prepositions of possession and prepositions of accompaniment by some Jordanian learners of English. A total of 53 Jordanian English Majors participated in the study from the department of English language at Princess Alia University College. In collecting…

  5. Effects of Music Instruction with Bamboo Xylophone Accompaniment on Singing Achievement among Second-Grade Children

    Science.gov (United States)

    Simeon, Jinky Jane C.; Ku, Agnes Chun Moi

    2015-01-01

    The aim of this research is to determine the effect of music instruction with bamboo xylophone as harmonic accompaniment on the singing achievement of second-grade children. Eighty children (N = 80) from four randomly selected classes in two different public schools in the city of Kota Kinabalu participated in this study and they were assigned to…

  6. 75 FR 61489 - Renewal of Declaration Regarding Emergency Use of Doxycycline Hyclate Tablets Accompanied by...

    Science.gov (United States)

    2010-10-05

    ... HUMAN SERVICES Office of the Secretary Renewal of Declaration Regarding Emergency Use of Doxycycline... use of doxycycline hyclate tablets accompanied by emergency use information subject to the terms of..., Michael O. Leavitt, declared an emergency justifying the authorization of the emergency use of...

  7. String Instrument Intonation in Upper and Lower Registers: The Effects of Accompaniment.

    Science.gov (United States)

    Kantorski, Vincent J.

    1986-01-01

    Investigated the effects of different accompaniment and register conditions on string instrumentalists' intonation performance of scalar passages. Both upper and lower register performances were significantly sharper than the computer-generated model. Violinist evidenced the least deviation, followed in order by violists, cellists, and bassists.…

  8. Handbook of Remedial or Developmental Activities to Accompany the Illinois Test of Psycholinguistic Abilities.

    Science.gov (United States)

    Brown, Drucilla, Comp.

    This handbook, intended to accompany the Illinois Test of Psycholinguistic Abilities, describes numerous remedial and developmental activities for perceptual motor and psychomotor skills. Observable classroom behaviors associated with various perceptual motor and psychomotor disabilities (visual-motor channel disability, auditory-vocal channel…

  9. Effects of Music Instruction with Bamboo Xylophone Accompaniment on Singing Achievement among Second-Grade Children

    Science.gov (United States)

    Simeon, Jinky Jane C.; Ku, Agnes Chun Moi

    2015-01-01

    The aim of this research is to determine the effect of music instruction with bamboo xylophone as harmonic accompaniment on the singing achievement of second-grade children. Eighty children (N = 80) from four randomly selected classes in two different public schools in the city of Kota Kinabalu participated in this study and they were assigned to…

  10. 76 FR 69738 - Revised 2011 Annual Telecommunications Reporting Worksheet (FCC Form 499-A) and Accompanying...

    Science.gov (United States)

    2011-11-09

    ... COMMISSION Revised 2011 Annual Telecommunications Reporting Worksheet (FCC Form 499-A) and Accompanying... Competition Bureau released the revised Telecommunications Reporting Worksheet (FCC Form 499-A) and... Competition Bureau, Telecommunications Access Policy Division, at (202) 418-7400 or via the Internet...

  11. Changes in Illness-Related Behavior and Dysphoria Accompanying Behavioral Treatment of Recurrent Headache.

    Science.gov (United States)

    Rains, Jeanetta C.; Lohr, Jeffrey M.

    Psychophysiologic research indicates that muscle-contraction headache often is not characterized by excessive or unusual muscular activity, and muscular changes seldom accompany headache improvement resulting from treatment. Multidimensional models have been proposed, which emphasize the interdependence of physiological and psychological systems…

  12. STUDY OF THE GAMMA EMISSION PROBABILITY ACCOMPANYING THE SPONTANEOUS FISSION OF CF-252

    NARCIS (Netherlands)

    VANDERPLOEG, H; LAURENS, CR; BACELAR, JCS; BUDA, A; GAARDHOJE, JJ; VANTHOF, G; KALANTARNAYESTANAKI, N; VANDERWOUDE, A; ZELAZNY, Z

    1994-01-01

    A study of the gamma emission accompanying the spontaneous fission process of Cf-252 has been performed. The photon emission probability between 3 and 70 MeV and its angular dependence with respect to the fission direction were measured. These measurements were performed as a function of the mass

  13. Accompanying Symptoms Overlap during Attacks in Menière’s Disease and Vestibular Migraine

    Science.gov (United States)

    Lopez-Escamez, Jose Antonio; Dlugaiczyk, Julia; Jacobs, Julien; Lempert, Thomas; Teggi, Roberto; von Brevern, Michael; Bisdorff, Alexandre

    2014-01-01

    Menière’s disease and vestibular migraine (VM) are the most common causes of spontaneous recurrent vertigo. The current diagnostic criteria for the two disorders are mainly based on patients’ symptoms, and no biological marker is available. When applying these criteria, an overlap of the two disorders is occasionally observed in clinical practice. Therefore, the present prospective multicenter study aimed to identify accompanying symptoms that may help to differentiate between MD, VM, and probable vestibular migraine (pVM). Two hundred and sixty-eight patients were included in the study (MD: n = 119, VM: n = 84, pVM: n = 65). Patients with MD suffered mainly from accompanying auditory symptoms (tinnitus, fullness of ear, and hearing loss), while accompanying migraine symptoms (migraine-type headache, photo-/phonophobia, visual aura), anxiety, and palpitations were more common during attacks of VM. However, it has to be noted that a subset of MD patients also experienced (migraine-type) headache during the attacks. On the other hand, some VM/pVM patients reported accompanying auditory symptoms. The female/male ratio was statistically higher in VM/pVM as compared to MD, while the age of onset was significantly lower in the former two. The frequency of migraine-type headache was significantly higher in VM as compared to both pVM and MD. Accompanying headache of any type was observed in declining order in VM, pVM, and MD. In conclusion, the present study confirms a considerable overlap of symptoms in MD, VM, and pVM. In particular, we could not identify any highly specific symptom for one of the three entities. It is rather the combination of symptoms that should guide diagnostic reasoning. The identification of common symptom patterns in VM and MD may help to refine future diagnostic criteria for the two disorders. PMID:25566172

  14. Characteristics of Extraordinary Religious Phenomena Accompanying the Christian Religious Experience—Reflection

    Directory of Open Access Journals (Sweden)

    Stanisław Głaz

    2014-12-01

    Full Text Available This paper presents an attempt to discuss in more detail the question of understanding of religious experience in the context of the Christian religion, as well as to show the characteristic extraordinary religious experiences accompanying many people during that experience. The question of experiences evoked by psychedelic drugs, such as the issue of a vision and chemical ecstasy, are not discussed here, and instead the author has chosen to present only typical phenomena accompanying religious experience, which are caused by God’s doing, like visions, ecstasy, glossolalia (speaking in tongues or stigmata. The paper also presents their role and contribution to the process of a human being’s religious development.

  15. Triple Peripheral Nerve Injury Accompanying to Traumatic Brain Injury: A Case Report

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    Ižlknur Can

    2014-02-01

    Full Text Available Secondary injuries especially extremity fractures may be seen concurrently with traumatic brain injury (TBI. Peripheral nerve damages may accompany to these fractures and may be missed out, especially in acute stage. In this case report; damage of radial, ulnar and median nerves which was developed secondarily to distal humerus fracture that could not be detected in acute stage, in a patient who had motor vehicle accident (MVA. 29-year-old male patient was admitted with weakness in the right upper extremity. 9 months ago, he had traumatic brain injury because of MVA, and fracture of distal humerus was detected in follow-ups. Upon the suspect of the peripheral nerve injury, the diagnosis was confirmed with ENMG. The patient responded well to the rehabilitation program treatment. In a TBI patient, it must be kept in mind that there might be a secondary trauma and therefore peripheral nerve lesions may accompany to TBI.

  16. HEREDITARY DISEASES AND SYNDROMES ACCOMPANIED BY FEBRILE CONVULSIONS: CLINICAL AND GENETIC CHARACTERISTICS AND DIAGNOSTIC PROCEDURES

    Directory of Open Access Journals (Sweden)

    E. L. Dadali

    2016-01-01

    Full Text Available The authors provide a review of the clinical and genetic characteristics of hereditary diseases and syndromes accompanied by febrile convulsions, which is illustrated by examples of their own observations. The paper sets forth the possibilities and limitations of using current methods for the molecular genetic diagnosis of idiopathic and symptomatic epilepsies. The most effective and less expensive technique of molecular genetic analysis is shown to be an exome sequencing test using the panels of genes responsible for the occurrence of diseases with simi1ar clinical symptoms. The paper also presents the structure of the panel of genes responsible for the occurrence of monogenic epilepsies, which has been designed at the Genomed Clinic and includes 448 genetic variants. It also determines the significance of using a chromosomal microarray analysis to diagnose both chromosomal and monogenic diseases accompanied by convulsions. 

  17. Grain growth and thermal stability accompanying recrystallization in undercooled Ni-3at.%Sn alloy

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Z., E-mail: chenzheng1218@163.com [School of Material Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116 (China); State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi' an, Shaanxi 710016 (China); Chen, Q.; Shen, C.J. [School of Material Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116 (China); Liu, F. [State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi' an, Shaanxi 710016 (China)

    2015-10-15

    The grain growth and thermal stability after recrystallization in as-solidified highly undercooled Ni-3at.%Sn alloy melt were investigated. As for undercooled Ni-3at.%Sn alloy, a transition from dendritic to granular crystals occurred when ΔT≥ΔT{sup *}, which was induced by the plastic deformation of dendrites and subsequent recrystallization. On this basis, the subsequent grain growth and solute segregation accompanying recalescence were calculated by a recently proposed thermo-kinetic model, which showed close agreement with the experimental results. It is concluded that the grain growth process was interrelated to recalescence, solute trapping and solute segregation of Sn atoms captured by solute trapping, which was responsible for the reduction of grain boundary energy and improvement of thermal stability. - Highlights: • A transition from dendritic to granular crystals occurred when ΔT≥ΔT{sup *}. • The grain growth accompanying recalescence was calculated. • A close agreement with the experimental results was shown.

  18. Quotidian of accompanying family members in an environment of care: the emergence of hospital tribes

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    Silvia da Silva Santos Passos

    2016-06-01

    Full Text Available ABSTRACT OBJECTIVE Understand the quotidian relationships of accompanying family members in an environment of care, which are close to the metaphor of a tribe in hospital environment. METHODQualitative study with data gathered from semi-structured interviews and observations with 16 family members accompanying hospitalized individuals with dependence on self-care. Data were submitted to thematic analysis, and analyzed through the metaphor of "tribe" proposed by comprehensive sociology. RESULTS Family members build up social clusters around caring, where we find traits typical of tribes: emotional ambience; solidarity based on links of sympathy and mutual assistance; an affectual nebula in the process of interaction; a logic of fusion in tactile relations; and communion/religiosity in the process of connecting in a collective identity. CONCLUSION In the presence of tragedy, families build social clusters similar to tribes having care as a totem.

  19. Pitch-informed solo and accompaniment separation towards its use in music education applications

    Science.gov (United States)

    Cano, Estefanía; Schuller, Gerald; Dittmar, Christian

    2014-12-01

    We present a system for the automatic separation of solo instruments and music accompaniment in polyphonic music recordings. Our approach is based on a pitch detection front-end and a tone-based spectral estimation. We assess the plausibility of using sound separation technologies to create practice material in a music education context. To better understand the sound separation quality requirements in music education, a listening test was conducted to determine the most perceptually relevant signal distortions that need to be improved. Results from the listening test show that solo and accompaniment tracks pose different quality requirements and should be optimized differently. We propose and evaluate algorithm modifications to better understand their effects on objective perceptual quality measures. Finally, we outline possible ways of optimizing our separation approach to better suit the requirements of music education applications.

  20. Prevalence of open angle glaucoma in accompanying first degree relatives of patients with glaucoma

    Directory of Open Access Journals (Sweden)

    Franciele Vegini

    2008-01-01

    Full Text Available OBJECTIVE: The aim of this study was to determine the prevalence of open angle glaucoma in first-degree relatives accompanying POAG patients during routine examination in a reference hospital. METHOD: First-degree relatives of primary open angle glaucoma patients who accompanied their relatives to the glaucoma service of a reference hospital were screened for glaucoma. RESULTS: One-hundred and one first-degree relatives were examined, of which 56.4% had never had their intraocular pressure measured. 10.9% had previously been diagnosed with glaucoma, and 5.9% were newly diagnosed during this study. CONCLUSIONS: The eye examination of first-degree relatives identified a significant percentage of individuals with glaucoma. Despite being first-degree relatives of glaucoma patients, 56.4% of the companions had never had their eye pressure measured, demonstrating a lack of awareness about this disease.

  1. Optical coherence tomographic evaluation of foveal hard exudates in patients with diabetic maculopathy accompanying macular detachment.

    Science.gov (United States)

    Ota, Masafumi; Nishijima, Kazuaki; Sakamoto, Atsushi; Murakami, Tomoaki; Takayama, Kohei; Horii, Takahiro; Yoshimura, Nagahisa

    2010-10-01

    To study morphologic changes of serous retinal detachment (SRD) and hyperreflective dots, which have been reported to be precursors of hard exudates, detectable in SRD using optical coherence tomography (OCT) to assess whether or not the OCT findings are correlated with the subfoveal deposition of hard exudates in patients with diabetic macular edema (DME) accompanied by SRD. Retrospective chart review. Twenty-eight eyes of 19 patients with DME accompanied by SRD. We imaged SRD and the hyperreflective dots in SRD using spectral domain OCT (SD-OCT). The number and distribution of the hyperreflective dots in SRD were evaluated before the initial treatment at our hospital for DME accompanied by SRD. Based on a difference in the SD-OCT findings, the study eyes were divided into 2 groups: eyes with a few dots and those with many dots. We studied the clinical course of these 2 groups to assess whether or not the findings of SRD and hyperreflective dots on the SD-OCT images were correlated with deposition of hard exudates in the subfoveal space during follow-up. Correlation of the SD-OCT findings of SRD and hyperreflective dots with deposition of hard exudates in the subfovea of patients with DME accompanied by SRD. Subfoveal deposition of hard exudates was seen in 11 of the 28 eyes at the final examination. Before initial treatment at our hospital, 14 eyes had a few hyperreflective dots SRD and 14 eyes had many hyperreflective dots. Whereas no deposition of hard exudates in the subfoveal space was seen in the former eyes, it was seen in 11 of the latter 14 eyes (P exudates during follow-up. Copyright © 2010 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  2. CLINICAL AND FUNCTIONAL FEATURES IN COURSE CHRONIC PANCREATITIS WITH ACCOMPANYING DUODENOSTASIS

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    Ya. M. Vahrushev

    2016-01-01

    Full Text Available The aim. Research the clinical features, functional state of duodenum among patients with chronic pancreatitis and accompanying duodenostasis.Materials and methods. The clinical course of chronic pancreatitis with accompanying duodenostasis (85 cases and isolated chronic pancreatitis (56 cases has been studied. Along with the general clinical data the study includes the results of exocrine pancreatic function examination (fecal elastase-1, blood alpha amylase and lipase and its endocrine function (insulin and С-peptide. Regulating hormonal factors (gastrin and somatotropin have been studied. Was used results of rentgenology and endoscopic examinations, intraduodenal manometry results in verification of duodenostasis.Results. In the observation group resistant pain syndrome was revealed in 93% cases (at patient with isolated pancreatitis in 57% cases and in more degree was expressed coprological syndromes (amilorhea in 82,29% cases, creatorhea in 82,14% cases, steatorhea in 87,5% cases. In the observation group were significantly more diagnosed hyperperistaltic (in 88% cases of observation group and in 9,4% cases of the comparison group and duodenal hypertension (in 22% cases of observation group and in 0.0% cases of the comparison group. The phenomenon of «semolina» was reveals more often in observation group (in 31,9% cases of observation group and in 5% cases of the comparison group.Among patients with chronic pancreatitis and accompanying duodenostasis decrease the level of C-peptid and increase secretion of insulin, somatotropin and gastrin.Conclusion. It reveals that according to the comprehensive clinical and functional investigation chronic pancreatitis with accompanying duodenostasis gets a more severe course in comparison with isolated pancreatitis.

  3. Multiple dental anomalies accompany unilateral disturbances in abducens and facial nerves: A case report

    Directory of Open Access Journals (Sweden)

    Elham Talatahari

    2016-01-01

    Full Text Available This article describes the oral rehabilitation of an 8-year-old girl with extensively affected primary and permanent dentition. This report is unique in which distinct dental anomalies including enamel hypoplasia, irregular dentin formation, taurodontism, hpodontia and dens in dente accompany unilateral disturbance of abducens and facial nerves which control the lateral eye movement, and facial expression, respectively.   Keywords: enamel hypoplasia; irregular dentin formation; taurodontism; hypodontia; dens in dente; abducens and facial nerves;

  4. HIFI: a computer code for projectile fragmentation accompanied by incomplete fusion

    Energy Technology Data Exchange (ETDEWEB)

    Wu, J.R.

    1980-07-01

    A brief summary of a model proposed to describe projectile fragmentation accompanied by incomplete fusion and the instructions for the use of the computer code HIFI are given. The code HIFI calculates single inclusive spectra, coincident spectra and excitation functions resulting from particle-induced reactions. It is a multipurpose program which can calculate any type of coincident spectra as long as the reaction is assumed to take place in two steps.

  5. Colonic perforation in a patient with systemic lupus erythematosus accompanied by cytomegalovirus infection: A case report

    OpenAIRE

    Yuichi Tachikawa; Hiroaki Nozawa; Junichiro Tanaka; Takeshi Nishikawa; Toshiaki Tanaka; Tomomichi Kiyomatsu; Keisuke Hata; Kazushige Kawai; Shinsuke Kazama; Hironori Yamaguchi; Soichiro Ishihara; Eiji Sunami; Joji Kitayama; Madoka Fujisawa; Katutoshi Takahashi

    2016-01-01

    Introduction: Cytomegalovirus (CMV) infection of the gastrointestinal tract is an uncommon illness, but can be observed in immunocompromised patients. Systemic lupus erythematosus (SLE) patients are generally at high risk of CMV infection. Here we report a subacute progressive case of colitis in SLE accompanied by cytomegalovirus infection. Presentation of case: The patient, a 79-year-old woman, was hospitalized complaining of fever, polyarthritis, and skin ulcer that had lasted seven days...

  6. EVALUATION OF INFLAMMATORY CHANGES IN JOINTS AT PATIENTS WITH HEMOPHILIA ACCOMPANIED RECURRENT HEMARTHROSIS

    OpenAIRE

    I. L. Davydkin; Y. A. Kosyakova; Y. V. Lartsev; A V Kapishnikov

    2012-01-01

    Objective: to search for new diagnostic approaches for estimating the prevalence of posthemorrhagic phase of inflammation in the joints of patients with hemophilia accompanied recurrent hemarthrosis. Using the method of computerized infrared thermography measured the temperature above the knee, ankle and lower leg. Analysis of the data held with a specially developed algorithm that forms the basis of a computer program for computer (2009). It is established that with increasing temperature ov...

  7. Surgical guidance system using hand-held probe with accompanying positron coincidence detector

    Energy Technology Data Exchange (ETDEWEB)

    Majewski, Stanislaw; Weisenberger, Andrew G.

    2017-10-10

    A surgical guidance system offering different levels of imaging capability while maintaining the same hand-held convenient small size of light-weight intra-operative probes. The surgical guidance system includes a second detector, typically an imager, located behind the area of surgical interest to form a coincidence guidance system with the hand-held probe. This approach is focused on the detection of positron emitting biomarkers with gamma rays accompanying positron emissions from the radiolabeled nuclei.

  8. Modified Latarjet Procedure for Patients with Glenoid Bone Defect Accompanied with Anterior Shoulder Instability.

    OpenAIRE

    Demirhan, Mehmet Selahattin; Atalar, Ata Can; Bilsel, Kerem; Eren, İlker; Çelik, Derya; Çil, Hilal

    2013-01-01

    Abstract: Objective: The aim of this study was to assess the effects of coracoid bone block (modified Latarjet) procedure on clinical and functional results in cases with glenoid bone defect accompanied with anterior shoulder instability. Methods: The study included 35 patients (average age: 35 years; range: 20 to 58 years) with glenoid bone defect and recurrent dislocations treated with the modified Latarjet procedure. There were 12 sports injuries, 5 post-epileptic cases and 18 recurrent an...

  9. A case of recurrent complex regional pain syndrome accompanying Raynaud's disease: a prospective coincidence?

    Science.gov (United States)

    Kesikburun, Serdar; Günendi, Zafer; Aydemir, Koray; Özgül, Ahmet; Tan, Arif Kenan

    2013-01-01

    Complex regional pain syndrome (CPRS) and Raynaud's disease are disorders characterized by vasomotor disturbances associating with abnormal autonomic nervous system. We present a case of CRPS involving a history of recurrence and no initiating event. Raynaud's disease accompanying CRPS was diagnosed clinically in the patient. We propose that a sympathetic dysfunction underlies the pathophysiologies of both disorders and may be responsible for the coexistence of these two distinct entities. Recurrence and unknown etiology of CRPS might account for temporary alterations in sympathetic function.

  10. A qualitative investigation into the characteristics and effects of music accompanying exercise

    OpenAIRE

    Priest, DL; Karageorghis, CI

    2008-01-01

    The purpose of the present study was to identify the characteristics of music used to accompany physical exercise and investigate the effects of such music using a qualitative approach. This work underpins the further development of a theoretical structure that is still relatively new. Semi-structured interviews were conducted with a sample of exercise participants (N = 13), seven males and six females, all with at least two years’ experience of exercising to music. An inductive content analy...

  11. Frontal mucocele with an accompanying orbital abscess mimicking a fronto-orbital mucocele: case report

    OpenAIRE

    Akkuzu Guzin; Aydin Erdinc; Akkuzu Babur; Bilezikci Banu

    2006-01-01

    Abstract Background Mucoceles are slowly expanding cystic lesions with respiratory epithelium containing mucus most commonly affecting the frontal and ethmoidal sinuses. They are caused by obstruction of sinus ostium. Mucoceles exert pressure on the bony boundaries and due to the proximity to the brain and orbit extension to these areas are common. Case presentation A case of a frontal mucocele with an accompanying orbital abscess mimicking a fronto-orbital mucocele is reported. A 77 year old...

  12. α-accompanied cold ternary fission of Pu-244238 isotopes in equatorial and collinear configuration

    Science.gov (United States)

    Santhosh, K. P.; Krishnan, Sreejith; Priyanka, B.

    2015-04-01

    The cold ternary fission of 238Pu, 240Pu, 242Pu, and 244Pu isotopes, with 4He as light charged particle, in equatorial and collinear configuration has been studied within the unified ternary fission model. The fragment combination 100Zr+4He+134Te possessing the near doubly magic nuclei 134Te(N =82 ,Z =52 ) gives the highest yield in the α-accompanied ternary fission of 238Pu. For the α-accompanied ternary fission of 240Pu, 242Pu, and 244Pu isotopes, the highest yield was found for the fragment combination with doubly magic nuclei 132Sn(N =82 ,Z =50 ) as the heavier fragment. The deformation and orientation of fragments have also been taken into account for the α-accompanied ternary fission of Pu-244238 isotopes, and it has been found that, in addition to the closed-shell effect, ground-state deformation also plays an important role in determining the isotopic yield in the ternary fission process. The emission probability and kinetic energy of long-range α particles have been calculated and are found to be in good agreement with the experimental data.

  13. [Longjintonglin Capsules for type IIIA prostatitis accompanied by abnormal semen liquefaction: A clinical observation].

    Science.gov (United States)

    Cai, Hong-cai; Wan, Chang-chun; Geng, Qiang; Liu, Wei; Zhang, Guo-wei; Shang, Xue-jun; Huang, Yu-feng

    2016-01-01

    To evaluate the therapeutic effect of Longjintonglin Capsules on type IIIA prostatitis accompanied by abnormal semen liquefaction. We selected 140 patients with type IIIA prostatitis accompanied by abnormal semen liquefaction according to the diagnostic standards of the American Institutes of Health (NIH) and treated them with Longjintonglin Capsules orally 3 capsules once tid for 12 weeks. We obtained the NIH Chronic Prostatitis Symptom Indexes (NIH-CPSI), traditional Chinese medicine (TCM) syndrome scores, leukocyte count in the expressed prostatic secretion (EPS), semen liquefaction time, and the results of semen analysis and compared these indicators before and after the treatment. Of the 140 cases, 132 were included in this study, excluding 8 due to their incomplete case histories. Before and after 4, 8 and 12 weeks of medication, the total NIH-CPSI scores were 24.52 ± 5.43, 21.28 ± 4.85, 18.01 ± 4.28, and 14.49 ± 3.65 (P Capsules, with its advantages of safety, effectiveness and no obvious adverse effects, deserve to be recommended for the treatment of type IIIA prostatitis accompanied by abnormal semen liquefaction.

  14. Mulberry hypertrophy and accompanying sinonasal pathologies: A review of 68 cases.

    Science.gov (United States)

    Akduman, Davut; Haksever, Mehmet; Yanilmaz, Muhammed; Solmaz, Fevzi

    2016-08-01

    Mulberry hypertrophy occasionally coexists with sinonasal pathologies. There are very few reports in the literature on this clinical entity. We conducted a retrospective study to draw attention to this condition in the context of accompanying sinonasal pathologies. Our study group was made up of 68 patients-51 males and 17 females, aged 13 to 57 years (mean: 34.9)-who had been diagnosed with mulberry hypertrophy and at least one accompanying sinonasal pathology. All patients had a long-standing chronic discharge. Forty-nine of these patients (72.1%) had unilateral mulberry hypertrophy. The most common concomitant pathologies were chronic rhinosinusitis and ostiomeatal complex disease; others included septal deviation, nasal polyposis, allergic rhinitis, and concha bullosa. Thirty-six patients (52.9%) with varying degrees of choanal/nasal obstruction were operated on with endoscopic excision to treat the mulberry hypertrophy. In all, most patients underwent some sort of surgery to treat either the mulberry hypertrophy or the accompanying sinonasal pathology. Based on our findings, we suggest a clinical staging system to serve as a way to standardize management and guide future basic and clinical research.

  15. Light charged particle accompanied ternary fission of {sup 242}Cm using the Coulomb and proximity potential

    Energy Technology Data Exchange (ETDEWEB)

    Santhosh, K.P.; Krishnan, Sreejith; Priyanka, B. [Kannur University, School of Pure and Applied Physics, Kerala (India)

    2014-04-15

    The cold ternary fission of {sup 242}Cm with {sup 4}He, {sup 10}Be and {sup 14}C as light charged particle has been studied by taking the interacting barrier as the sum of Coulomb and proximity potential with the fragments in equatorial configuration. The favorable fragment combinations are obtained from the cold valley plot (plot of driving potential vs. mass number of fragments) and by calculating the yield for charge minimized fragments. The maximum yield in the {sup 4}He accompanied ternary fission is obtained for the fragmentation channel {sup 104}Mo + {sup 134}Te + {sup 4}He and for the {sup 10}Be accompanied ternary fission, the maximum yield is found for the fragmentation channel {sup 98}Zr + {sup 134}Te + {sup 10}Be. It is to be noted that, in the case of {sup 14}C accompanied ternary fission, the maximum yield is obtained for the fragmentation channel {sup 94}Sr + {sup 134}Te + {sup 14}C and the next higher yield is found for the fragmentation channel {sup 96}Zr + {sup 132}Sn + {sup 14}C. Thus, the fragment combinations with maximum yields reveal the role of doubly magic and near doubly magic nuclei in cold ternary fission. (orig.)

  16. The strain of an accompanying conspecific affects the efficacy of social buffering in male rats.

    Science.gov (United States)

    Nakamura, Kayo; Ishii, Akiko; Kiyokawa, Yasushi; Takeuchi, Yukari; Mori, Yuji

    2016-06-01

    Social buffering is a phenomenon in which stress in an animal is ameliorated when the subject is accompanied by a conspecific animal(s) during exposure to distressing stimuli. We previously reported that in male Wistar rats, the presence of another Wistar rat mitigates conditioned fear responses to an auditory conditioned stimulus (CS). Subsequent analyses revealed several characteristics of this social buffering of conditioned fear responses. However, information regarding the specificity of accompanying conspecifics is still limited. In the present study, we assessed whether rats of other strains could induce social buffering in Wistar rats. When a fear-conditioned Wistar subject was re-exposed to the CS alone, we observed increased freezing and decreased investigation and walking, as well as elevated corticosterone levels. The presence of a Wistar, Sprague-Dawley, or Long-Evans rat blocked these responses, suggesting that social buffering was induced by these strains of rats. In contrast, a Fischer 344 rat did not induce social buffering in the Wistar subject. We further found that an inbred Lewis rat induced social buffering whereas a Brown Norway rat, a strain that has been established independently from Wistar rats, did not. These results suggest that the difference in origin, rather than the inbred or outbred status of the associate rat, seemed to account for the lack of social buffering induced by the F344 rats. Based on these findings, we conclude that strains of an accompanying conspecific can affect the efficacy of social buffering in rats.

  17. Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae.

    Science.gov (United States)

    Thrower, Douglas A; Stemple, Jennifer; Yeh, Elaine; Bloom, Kerry

    2003-02-01

    Dicentric chromosomes undergo breakage during mitosis as a result of the attachment of two centromeres on one sister chromatid to opposite spindle poles. Studies utilizing a conditional dicentric chromosome III in Saccharomyces cerevisiae have shown that dicentric chromosome repair occurs primarily by deletion of one centromere via a RAD52-dependent recombination pathway. We report that dicentric chromosome resolution requires RAD1, a gene involved in the single-strand annealing DNA repair pathway. We additionally show that single-strand annealing repair of a dicentric chromosome can occur in the absence of RAD52. RAD52-independent repair requires the adaptation-defective cdc5-ad allele of the yeast polo kinase and the DNA damage checkpoint gene RAD9. Dicentric chromosome breakage in cdc5-ad rad52 mutant cells is associated with a prolonged mitotic arrest, during which nuclei undergo microtubule-dependent oscillations, accompanied by dynamic changes in nuclear morphology. We further demonstrate that the frequency of spontaneous direct repeat recombination is suppressed in yeast cells treated with benomyl, a drug that perturbs microtubules. Our findings indicate that microtubule-dependent processes facilitate recombination.

  18. [Physiognomy-accompanying auditory hallucinations in schizophrenia: psychopathological investigation of 10 patients].

    Science.gov (United States)

    Nagashima, Hideaki; Kobayashi, Toshiyuki

    2010-01-01

    We previously reported two schizophrenic patients with characteristic hallucinations consisting of auditory hallucinations accompanied by visual hallucinations of the speaker's face. The patient sees the face of the hallucinatory speaker in his/her mind and hears the voice talking inwardly. We termed these experiences physiognomy-accompanying auditory hallucinations. In this report, we present 10 patients with schizophrenia showing physiognomy-accompanying auditory hallucinations and evaluate the characteristics of these clinical symptoms. Moreover we consider what the symptoms mean for patients and the metabasis from structural aspects. Lastly, we consider how we can treat these patients living autistic lives with the symptoms. During physiognomy-accompanying auditory hallucinations, the realistic face moves its mouth and talks to the patient expressively. In early onset cases, the faces of various real people appear talking about ordinary things while in late onset cases, the faces can be imaginary but are mainly real people talking about ordinary or delusional things. We suppose that these characteristics of the symptoms unify the schizophrenic world overwhelmed by "a force of non-sense" to "the sense field". "The force of non-sense" is a substantial power but cannot be reduced to the real meaning. And we suppose that not visual reality but the intensity of auditory hallucinations of the face brings about the overwhelming intensity of symptoms and the substantiality of this intensity depends on the states of excessive fullness of "the force of non-sense". With these symptoms patients see the narration of auditory hallucinations through the facial image and the content of auditory hallucinations is compressed into the movement of visual hallucinations of the speaker's face. The form of symptoms is realistic but the speaker's face and voice are beyond ordinary time and space. The symptoms are essentially different from ordinary perception. The visual

  19. [About the last transit. Accompaniment of the soul and the personality].

    Science.gov (United States)

    Pilar Fenés, María

    2013-03-01

    "Death and its approach represent a field of study for nurses": thus ended his article Montserrat Salvador Borrell in Revista ROL de Enfermería in November 2010. Address it also from the point of view of the soul brings perspective and gives horizon in the sense of one greater understanding. Death, contemplated and lived exclusively from the material side, flees from as a not only traumatic event for which the experience but also unsurpassed for loved ones. Paradoxically, it's a more natural and frequent phenomenon. Why humans so hard that naturalness? Because we have not cultivated, sometimes not even recognized, other spiritual nature which forms us and who has the key to give sense to the decisive events. For many spiritual traditions, death is birth to another world that, from here, we call beyond. In accompaniment of soul seminars, based on the teachings transmitted by Elisabeth Kübler Ros and Marie Lise Labonte, patterns are learned to accompany the personality and soul--which does not always go according to the time of split--and to help the soul to rediscover his way toward the light, toward the origin. The soul knows it well, but the emotional ties forged during his earthly journey can retain it and hinder the process, which becomes slow and painful. The accompaniment of the soul is a "service" human, social and spiritual. Guides in a forgotten and often feared territory is a relief. You have guides to prepare the great journey allows, especially, make peace with himself with others I with the world. The Egyptians used the symbol of the solar boat, which waits for the deceased to cross the great waters. Nurses are, unknowingly, barqueras of small and large transits of existence.

  20. Learning to sing with the accompaniment of the gusle in Serbia in the XXI century

    Directory of Open Access Journals (Sweden)

    Lajić-Mihajlović Danka

    2012-01-01

    Full Text Available In this paper, learning to sing with the accompaniment of the gusle is reconsidered within the concept of the preservation of traditional folk music as an intangible cultural heritage, that is, as a specific form of music education. Having dealt with a traditional way of providing continuity in the practice of guslars` (i. e. gusle players - which is learning by observing and imitating more experienced family members or guslars from nearby surrounding - some crucial moments in the history of singing with the accompaniment of the gusle, which also influenced the process of transmitting this skill, are demonstrated. In recent times, singing with the accompaniment of the gusle has been mastered not only within family circles but also in associations of guslars, at private schools of music, and lately there has also been an opportunity to learn this skill at some state schools of music. Accordingly, interviews with the members of an Association of guslars association that is particularly distinguished by the fostering young guslars, and interviews with gusle teachers and pupils who attend schools of music were used as primary sources for this paper, together with the specific experience of the author as a president of the jury in a young guslars’ competition. Since the poetic content of the songs is primarily learnt from printed collections or audio recordings, future guslars in fact master the musical skills of playing and singing along the instrument, that is, the skill of the vocal interpretation of a chosen written poetic text. Concerning these, some programmes and methods are discussed, as well as their effects on young guslars’ competence, principally from the point of style and aesthetics. Attention is particularly drawn to flaws in the teaching concept of future gusle players at state schools of music. In conclusion, the necessity of applying ethnomusicological findings to activities concerning the preservation of an intangible cultural

  1. Accompanying conditions in patients with chronic spontaneous urticaria and urticarial vasculitis: Results of a retrospective study

    Directory of Open Access Journals (Sweden)

    Sevgi Akarsu

    2015-03-01

    Full Text Available Background and Design: Chronic spontaneous urticaria (CSU, the most common form of chronic urticaria, is characterized by spontaneous wheals and/or angioedema lasting longer than six weeks. Urticarial vasculitis (UV is a small vessel vasculitis; but is also included in the various classification systems of chronic urticaria by some authors. The aim of our study was to evaluate the frequency of accompanying conditions, and to compare the demographic, clinical and laboratory features of patients with CSU and UV. Materials and Methods: In this study, the files of CSU (n=146 and UV (n=43 patients who had been hospitalized between January 2010-December 2013 were retrospectively reviewed in terms of demographic data, disease-specific history, accompanying diseases, medications and laboratory findings. Results: When comparing between the two groups for statistical differences, it was found that the frequencies of personal atopy and angioedema were significantly higher in patients with CSU; however, the patients with UV had significantly higher rates for female gender and the presence of systemic symptoms compared to those with CSU. It was determined that the mean values for erythrocyte sedimentation rate and C-reactive protein, as well as the rates of antinuclear antibody positivity and hypocomplementemia were significantly higher in the UV group, compared with those in the CSU group. Accompanying factors were determined as infections, medications, autoimmune diseases and malignities in order of frequency in both CSU and UV groups, but only the frequency of autoimmune diseases was significantly higher in patients with UV group. Conclusion: Although various authors have reported that a large number (up to 40-60% of patients with UV may present only with wheals (clinically indistinguishable from CSU; we assumed that it would be more appropriate to be considered UV as a priority in patients especially who had systemic symptom, autoimmune disease

  2. 78 FR 69415 - Proposed Changes to FCC Form 499-A, FCC Form 499-Q, and Accompanying Instructions.

    Science.gov (United States)

    2013-11-19

    ... COMMISSION Proposed Changes to FCC Form 499-A, FCC Form 499-Q, and Accompanying Instructions. AGENCY: Federal... to (1) the annual Telecommunications Reporting Worksheet, FCC Form 499-A (Form 499-A) and... quarterly Telecommunications Reporting Worksheet, FCC Form 499-Q (Form 499-Q) and accompanying...

  3. Personality Pathology of Adults with Autism Spectrum Disorder without Accompanying Intellectual Impairment in Comparison to Adults with Personality Disorders

    Science.gov (United States)

    Strunz, Sandra; Westphal, Linda; Ritter, Kathrin; Heuser, Isabella; Dziobek, Isabel; Roepke, Stefan

    2015-01-01

    Differentiating autism spectrum disorders (ASDs) without accompanying intellectual impairment from personality disorders is often challenging. Identifying personality traits and personality pathology specific to ASD might facilitate diagnostic procedure. We recruited a sample of 59 adults with ASD without accompanying intellectual impairment, 62…

  4. Personality Pathology of Adults with Autism Spectrum Disorder without Accompanying Intellectual Impairment in Comparison to Adults with Personality Disorders

    Science.gov (United States)

    Strunz, Sandra; Westphal, Linda; Ritter, Kathrin; Heuser, Isabella; Dziobek, Isabel; Roepke, Stefan

    2015-01-01

    Differentiating autism spectrum disorders (ASDs) without accompanying intellectual impairment from personality disorders is often challenging. Identifying personality traits and personality pathology specific to ASD might facilitate diagnostic procedure. We recruited a sample of 59 adults with ASD without accompanying intellectual impairment, 62…

  5. Music Supports the Processing of Song Lyrics and Changes their Contents: : Effects of Melody, Silences and Accompaniment

    NARCIS (Netherlands)

    Schotanus, Yke

    2016-01-01

    A growing body of evidence indicates that singing might affect the perception of language both in a supportive and in a detrimental way. Accompaniment might be a factor of interest, because loud rests (on-beat silences) and out-of-key notes may confuse listeners, while accompaniment might elucidate

  6. 30 CFR 250.245 - What hydrogen sulfide (H2S) information must accompany the DPP or DOCD?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What hydrogen sulfide (H2S) information must... Documents (docd) § 250.245 What hydrogen sulfide (H2S) information must accompany the DPP or DOCD? The following H2S information, as applicable, must accompany your DPP or DOCD: (a) Concentration. The estimated...

  7. Music Supports the Processing of Song Lyrics and Changes their Contents: : Effects of Melody, Silences and Accompaniment

    NARCIS (Netherlands)

    Schotanus, Yke

    2016-01-01

    A growing body of evidence indicates that singing might affect the perception of language both in a supportive and in a detrimental way. Accompaniment might be a factor of interest, because loud rests (on-beat silences) and out-of-key notes may confuse listeners, while accompaniment might elucidate

  8. Solutions manual to accompany An introduction to numerical methods and analysis

    CERN Document Server

    Epperson, James F

    2014-01-01

    A solutions manual to accompany An Introduction to Numerical Methods and Analysis, Second Edition An Introduction to Numerical Methods and Analysis, Second Edition reflects the latest trends in the field, includes new material and revised exercises, and offers a unique emphasis on applications. The author clearly explains how to both construct and evaluate approximations for accuracy and performance, which are key skills in a variety of fields. A wide range of higher-level methods and solutions, including new topics such as the roots of polynomials, sp

  9. The emission of atoms and molecules accompanying fracture of single-crystal MgO

    Science.gov (United States)

    Dickinson, J. T.; Jensen, L. C.; Mckay, M. R.; Freund, F.

    1986-01-01

    The emission of particles due to deformation and fracture of materials has been investigated. The emission of electrons (exoelectron emission), ions, neutral species, photons (triboluminescence), as well as long wavelength electromagnetic radiation was observed; collectively these emissions are referred to as fractoemission. This paper describes measurements of the neutral emission accompanying the fracture of single-crystal MgO. Masses detected are tentatively assigned to the emission of H2, CH4, H2O, CO, O2, CO2, and atomic Mg. Other hydrocarbons are also observed. The time dependencies of some of these emissions relative to fracture are presented for two different loading conditions.

  10. [Patients at the end of life in the intensive care unit: cultural aspects of accompaniment].

    Science.gov (United States)

    Grom, I-U; Vagts, D A; Kampa, U; Pfeiffer, G; Schreiber-Winzig, L; Wiese, C H R

    2013-06-01

    The accompaniment of people in the face of death offers insights into dimensions which are mostly not seen in ordinary life. These insights also exist in intensive care in German hospitals and are highly relevant in medical decision making. End-of-life decisions in particular often determine medical, cultural and spiritual aspects concerning medical treatment and therapeutic targets and if necessary new therapy targets. The following article especially illuminates cultural aspects and their characteristics in patients at the end of life in the intensive care unit.

  11. Influence of Accompanying Anions on Potassium Retention and Leaching in Potato Growing Alluvial Soils

    Institute of Scientific and Technical Information of China (English)

    V.SHARMA; K.N.SHARMA

    2013-01-01

    A column study was carried out to assess the influence of accompanying anions on potassium (K) leaching at potato growing sites with different soil textures (sandy loam and clay loam) in northwestern India.Potassium was applied in the top 15 cm layer of soil column at 30 and 60 mg K kg-1 through different sources having different accompanying anions (Cl-,SO42-,NO3-and H2PO4-).Maximum K was retained in the top 0-15 cm layer with a sharp decrease in K content occurring in 15-30 cm layer of the soil column.The trend was similar for both levels of applied K as well as frequency of leaching and soil type.The decrease of K content in soil column after four leaching events was maximum in case of Khanaura sandy loam,while only minor decrease was observed in Hundowal clay loam when K was applied at 60 mg K kg-1,indicating higher potential of clay rich soil to adsorb K.In general,the K leaching in presence of the accompanying anions followed the order of SO42-≤ H2PO42-< NO3-=Cl-.Highest 1 mol L-1 CH3COONH4-extractable K was retained when K was applied along with SO42-and H2PO4-anions,and the least was retained when accompanying anion was Cl-.The influence of anions was more pronounced in the light textured soil and at high amounts of K application.Higher levels of K application resulted in higher losses of K,especially in sandy loam soil as observed from the leachate concentration.Among the different K sources,the maximum amount of K leaching was noticed in the soil column amended with KCl.After four leachings,the maximum amount of K leached out was 6.40 mg L-1 in Hundowal clay loam and 9.29 mg L-1 in Khanaura sandy loam at 60 mg K kg-1 of soil application through KCl.These concentrations were lower than the recommended guideline of the World Health Organisation (12.00 mg L-1).

  12. Solutions manual to accompany Statistics and probability with applications for engineers and scientists

    CERN Document Server

    Gupta, Bhisham C

    2013-01-01

    A solutions manual to accompany Statistics and Probability with Applications for Engineers and ScientistsUnique among books of this kind, Statistics and Probability with Applications for Engineers and Scientists covers descriptive statistics first, then goes on to discuss the fundamentals of probability theory. Along with case studies, examples, and real-world data sets, the book incorporates clear instructions on how to use the statistical packages Minitab® and Microsoft® Office Excel® to analyze various data sets. The book also features: Detailed discussions on sampling distributions, stati

  13. X-ray microbeam quantification of grain subdivision accompanying large deformations of copper

    Energy Technology Data Exchange (ETDEWEB)

    Butler, G.C.; Guvenilir, A.; McDowell, D.L.; Stock, S.R. [Georgia Inst. of Tech., Atlanta, GA (United States)

    1998-12-31

    Polychromatic synchrotron x-ray microbeams offer a very efficient alternative to electron beam methods for quantifying the amount and character of grain subdivision accompanying large deformations. With a 0.01 mm diameter collimator, bending magnet radiation from a 3.0 GeV source and image storage plates, samples of copper with thicknesses greater than 0.1 mm have been studied. Results from an as-received sample and a sample deformed to 100% torsion are compared and illustrate how efficiently grain subdivision can be quantified with polychromatic microbeam diffraction.

  14. Production of jets accompanied by W and Z bosons at LHC startup

    CERN Document Server

    Dobur, Didar

    2009-01-01

    We report on potential for measurement of W and Z boson production accompanied by jets. Of particular interest are jet multiplicity and $P_{\\rm T}$ distributions. The 10 to 100 $pb^{-1}$ datasets expected in the startup year of operation of LHC are likely to already provide information beyond the reach of the Tevatron collider both in jet multiplicity and $P_{\\rm T}$ range. We are especially interested in understanding the ratios of W+jets to Z+jets distributions by comparing them to next-to-leading order Monte Carlo generators, as these processes present a formidable background for searches of new physics phenomena.

  15. Production of e+ e- pairs accompanied by nuclear dissociation in ultraperipheral heavy-ion collisions

    Science.gov (United States)

    Adams, J.; Aggarwal, M. M.; Ahammed, Z.; Amonett, J.; Anderson, B. D.; Arkhipkin, D.; Averichev, G. S.; Bai, Y.; Balewski, J.; Barannikova, O.; Barnby, L. S.; Baudot, J.; Bekele, S.; Belaga, V. V.; Bellwied, R.; Berger, J.; Bezverkhny, B. I.; Bharadwaj, S.; Bhatia, V. S.; Bichsel, H.; Billmeier, A.; Bland, L. C.; Blyth, C. O.; Bonner, B. E.; Botje, M.; Boucham, A.; Brandin, A.; Bravar, A.; Bystersky, M.; Cadman, R. V.; Cai, X. Z.; Caines, H.; Sánchez, M. Calderón; Carroll, J.; Castillo, J.; Cebra, D.; Chajecki, Z.; Chaloupka, P.; Chattopdhyay, S.; Chen, H. F.; Chen, Y.; Cheng, J.; Cherney, M.; Chikanian, A.; Christie, W.; Coffin, J. P.; Cormier, T. M.; Cramer, J. G.; Crawford, H. J.; Das, D.; Das, S.; Moura, M. M.; Derevschikov, A. A.; Didenko, L.; Dietel, T.; Dong, W. J.; Dong, X.; Draper, J. E.; Du, F.; Dubey, A. K.; Dunin, V. B.; Dunlop, J. C.; Mazumdar, M. R.; Eckardt, V.; Edwards, W. R.; Efimov, L. G.; Emelianov, V.; Engelage, J.; Eppley, G.; Erazmus, B.; Estienne, M.; Fachini, P.; Faivre, J.; Fatemi, R.; Fedorisin, J.; Filimonov, K.; Filip, P.; Finch, E.; Fine, V.; Fisyak, Y.; Foley, K. J.; Fomenko, K.; Fu, J.; Gagliardi, C. A.; Gans, J.; Ganti, M. S.; Gaudichet, L.; Geurts, F.; Ghazikhanian, V.; Ghosh, P.; Gonzalez, J. E.; Grachov, O.; Grebenyuk, O.; Grosnick, D.; Guertin, S. M.; Guo, Y.; Gupta, A.; Gutierrez, T. D.; Hallman, T. J.; Hamed, A.; Hardtke, D.; Harris, J. W.; Heinz, M.; Henry, T. W.; Hepplemann, S.; Hippolyte, B.; Hirsch, A.; Hjort, E.; Hoffmann, G. W.; Huang, H. Z.; Huang, S. L.; Hughes, E. W.; Humanic, T. J.; Igo, G.; Ishihara, A.; Jacobs, P.; Jacobs, W. W.; Janik, M.; Jiang, H.; Jones, P. G.; Judd, E. G.; Kabana, S.; Kang, K.; Kaplan, M.; Keane, D.; Khodyrev, V. Yu.; Kiryluk, J.; Kisiel, A.; Kislov, E. M.; Klay, J.; Klein, S. R.; Klyachko, A.; Koetke, D. D.; Kollegger, T.; Kopytine, M.; Kotchenda, L.; Kramer, M.; Kravtsov, P.; Kravtsov, V. I.; Krueger, K.; Kuhn, C.; Kulikov, A. I.; Kumar, A.; Kunz, C. L.; Kutuev, R. Kh.; Kuznetsov, A. A.; Lamont, M. A.; Landgraf, J. M.; Lange, S.; Laue, F.; Lauret, J.; Lebedev, A.; Lednicky, R.; Lehocka, S.; Levine, M. J.; Li, C.; Li, Q.; Li, Y.; Lindenbaum, S. J.; Lisa, M. A.; Liu, F.; Liu, L.; Liu, Q. J.; Liu, Z.; Ljubicic, T.; Llope, W. J.; Long, H.; Longacre, R. S.; Lopez-Noriega, M.; Love, W. A.; Lu, Y.; Ludlam, T.; Lynn, D.; Ma, G. L.; Ma, J. G.; Ma, Y. G.; Magestro, D.; Mahajan, S.; Mahapatra, D. P.; Majka, R.; Mangotra, L. K.; Manweiler, R.; Margetis, S.; Markert, C.; Martin, L.; Marx, J. N.; Matis, H. S.; Matulenko, Yu. A.; McClain, C. J.; McShane, T. S.; Meissner, F.; Melnick, Yu.; Meschanin, A.; Miller, M. L.; Milosevich, Z.; Minaev, N. G.; Mironov, C.; Mischke, A.; Mishra, D.; Mitchell, J.; Mohanty, B.; Molnar, L.; Moore, C. F.; Mora-Corral, M. J.; Morozov, D. A.; Morozov, V.; Munhoz, M. G.; Nandi, B. K.; Nayak, T. K.; Nelson, J. M.; Netrakanti, P. K.; Nikitin, V. A.; Nogach, L. V.; Norman, B.; Nurushev, S. B.; Odyniec, G.; Ogawa, A.; Okorokov, V.; Oldenburg, M.; Olson, D.; Pal, S. K.; Panebratsev, Y.; Panitkin, S. Y.; Pavlinov, A. I.; Pawlak, T.; Peitzmann, T.; Perevoztchikov, V.; Perkins, C.; Peryt, W.; Petrov, V. A.; Phatak, S. C.; Picha, R.; Planinic, M.; Pluta, J.; Porile, N.; Porter, J.; Poskanzer, A. M.; Potekhin, M.; Potrebenikova, E.; Potukuchi, B. V.; Prindle, D.; Pruneau, C.; Putschke, J.; Rai, G.; Rakness, G.; Raniwala, R.; Raniwala, S.; Ravel, O.; Ray, R. L.; Razin, S. V.; Reichhold, D.; Reid, J. G.; Renault, G.; Retiere, F.; Ridiger, A.; Ritter, H. G.; Roberts, J. B.; Rogachevskiy, O. V.; Romero, J. L.; Rose, A.; Roy, C.; Ruan, L.; Sakrejda, I.; Salur, S.; Sandweiss, J.; Savin, I.; Sazhin, P. S.; Schambach, J.; Scharenberg, R. P.; Schmitz, N.; Schroeder, L. S.; Schweda, K.; Seger, J.; Seyboth, P.; Shahaliev, E.; Shao, M.; Shao, W.; Sharma, M.; Shen, W. Q.; Shestermanov, K. E.; Shimanskiy, S. S.; Simon, F.; Singaraju, R. N.; Skoro, G.; Smirnov, N.; Snellings, R.; Sood, G.; Sorensen, P.; Sowinski, J.; Speltz, J.; Spinka, H. M.; Srivastava, B.; Stadnik, A.; Stanislaus, T. D.; Stock, R.; Stolpovsky, A.; Strikhanov, M.; Stringfellow, B.; Suaide, A. A.; Sugarbaker, E.; Suire, C.; Sumbera, M.; Surrow, B.; Symons, T. J.; Toledo, A. Szanto; Szarwas, P.; Tai, A.; Takahashi, J.; Tang, A. H.; Tarnowsky, T.; Thein, D.; Thomas, J. H.; Timoshenko, S.; Tokarev, M.; Trainor, T. A.; Trentalange, S.; Tribble, R. E.; Tsai, O.; Ulery, J.; Ullrich, T.; Underwood, D. G.; Urkinbaev, A.; Buren, G. Van; Leeuwen, M. Van; Molen, A. M.; Varma, R.; Vasilevski, I. M.; Vasiliev, A. N.; Vernet, R.; Vigdor, S. E.; Viyogi, V. P.; Vokal, S.; Voloshin, S. A.; Vznuzdaev, M.; Waggoner, B.; Wang, F.; Wang, G.; Wang, G.; Wang, X. L.; Wang, Y.; Wang, Y.; Wang, Z. M.; Ward, H.; Watson, J. W.; Webb, J. C.; Wells, R.; Westfall, G. D.; Wetzler, A.; Whitten, C.; Wieman, H.; Wissink, S. W.; Witt, R.; Wood, J.; Wu, J.; Xu, N.; Xu, Z.; Xu, Z. Z.; Yamamoto, E.; Yepes, P.; Yurevich, V. I.; Zanevsky, Y. V.; Zhang, H.; Zhang, W. M.; Zhang, Z. P.; Zolnierczuk, P. A.; Zoulkarneev, R.; Zoulkarneeva, Y.; Zubarev, A. N.

    2004-09-01

    We present data on e+ e- pair production accompanied by nuclear breakup in ultraperipheral gold-gold collisions at a center of mass energy of 200 GeV per nucleon pair. The nuclear breakup requirement selects events at small impact parameters, where higher-order diagrams for pair production should be enhanced. We compare the data with two calculations: one based on the equivalent photon approximation, and the other using lowest-order quantum electrodynamics (QED). The data distributions agree with both calculations, except that the pair transverse momentum spectrum disagrees with the equivalent photon approach. We set limits on higher-order contributions to the cross section.

  16. A typical case of hydrallantois accompanied by fetal monstrosity in a local ewe of Kashmir

    Directory of Open Access Journals (Sweden)

    Hiranya Kumar Bhattacharyya

    2012-09-01

    Full Text Available A full termed local ewe with the history of continuous straining with labored breathing for last 24 hours was presented. The animal was disinclined to move with tense and round abdomen which developed rapidly during last two weeks. Caesarean section revealed hydrallantois accompanied by multiple fetal congenital abnormalities. The ewe was under observation for four weeks. Metritis developed 12 days post-operation and was treated successfully. The ewe was found active on 25 days post-surgery with gain of extra 3 kg bodyweight.

  17. The structure and energetics of midlatitude disturbances accompanying cold-air outbreaks over East Asia

    Science.gov (United States)

    Lau, N.-C.; Lau, K.-M.

    1984-01-01

    The evolution of extratropical transient waves as they propagate eastward from the Eurasian land mass toward the Pacific during selected cold surge events in the winter Monsoon Experiment (MONEX) is studied. The outstanding cold surge episodes during MONEX are first identified, and the salient synoptic features related to these events are described using composite streamline charts. The structure of rapidly varying disturbances accompanying the cold surges and the associated energetics are examined, and the behavior of those fluctuations over relatively longer time scales is addressed.

  18. Solutions manual to accompany Classical geometry Euclidean, transformational, inversive, and projective

    CERN Document Server

    Leonard, I E; Liu, A C F; Tokarsky, G W

    2014-01-01

    Solutions Manual to accompany Classical Geometry: Euclidean, Transformational, Inversive, and Projective Written by well-known mathematical problem solvers, Classical Geometry: Euclidean, Transformational, Inversive, and Projective features up-to-date and applicable coverage of the wide spectrum of geometry and aids readers in learning the art of logical reasoning, modeling, and proof. With its reader-friendly approach, this undergraduate text features self-contained topical coverage and provides a large selection of solved exercises to aid in reader comprehension. Material in this text can be tailored for a one-, two-, or three-semester sequence.

  19. [Multi-center study on the treatment of sudden deafness accompanied with tinnitus].

    Science.gov (United States)

    Zhao, Hui; Dong, Hong; Cheng, Yan; Ma, Yuanxu; Lin, Peng; Zhong, Shixun; Kang, Houyong; Qian, Yi; Hu, Guohua

    2015-06-01

    To investigate the clinical characteristics and the effects of different drug therapies in patients of sudden deafness accompanied with tinnitus. The international standardized clinical research methods, the unified design and program were used in the study. The patients of sudden deafness accompanied with tinnitus, aged between 18 to 65 years old, were recruited, whose duration was less than two weeks with no medication. The patients were divided into four types according to the hearing curve: type A was acute sensorineural hearing loss in low tone frequencies, type B was acute sensorineural hearing loss in high tone frequencies, type C was acute sensorineural hearing loss in all frequencies and type D was total deafness. Each type had four different treatment programs, based on the unified designed randomized table. A total of 1024 cases with single side sudden deafness were recruited in the study by 33 hospitals in China from August 2007 to October 2011. Among the 1024 cases, 922 cases were accompanied with tinnitus (90.04%). By classification of audiogram, among the 922 cases, 169 cases were type A (82.44%), 127 cases were type B (90.07%), 370 cases were type C (92.04%), and 256 cases were type D (92.75%). The tinnitus mostly was persistent and low tone tinnitus. The degree of the tinnitus was mostly 2-3 grade. The curative effects of different types were analyzed, type A had the highest rate of 96.18%, type C was 87.75%, type B was 81.51%, and type D had the lowest rate of 75.32%. Significant difference of curative rate between different types was detected (χ² = 125.33, P = 0.000). There had no significant difference between the four different treatment groups (all P > 0.05). In the cases with single side sudden deafness accompanied with tinnitus, the type in low tone frequencies has the best curative effect, followed by the type in all frequencies. The type in high tone frequencies and the total deafness type have poor curative results. The steroid plays a

  20. The Rate for $B\\bbar$ Production Accompanied by a Single Pion

    CERN Document Server

    Randall, Lisa; Randall, Lisa; Sather, Eric

    1993-01-01

    We study the rate for the production of ${B B^\\pm \\pi^\\mp}$, where the sign of the charged pion tags the flavor content of the neutral $B$ meson. We estimate this branching ratio, employing the heavy meson chiral effective theory. We find that at center of mass energy of approximately 12 GeV, a $B$ meson pair should be produced as often with and without an accompanying charged pion. We also calculate two pion production at this center of mass energy, and find that it is negligible, as is the rate for rho production. We consider the implications for CP violation studies. (Invited Talk, 1993 Electroweak Rencontres de Moriond)

  1. Solutions manual to accompany Combinatorial reasoning an introduction to the art of counting

    CERN Document Server

    DeTemple, Duane

    2014-01-01

    This is a solutions manual to accompany Combinatorial Reasoning: An Introduction to the Art of CountingWritten by well-known scholars in the field, Combinatorial Reasoning: An Introduction to the Art of Counting introduces combinatorics alongside modern techniques, showcases the interdisciplinary aspects of the topic, and illustrates how to problem solve with a multitude of exercises throughout. The authors'' approach is very reader-friendly and avoids the ""scholarly tone"" found in many books on this topic.  

  2. In situ electrochemical scanning tunneling microscopy investigation of renewing graphite surface accompanied by electrochemical reaction

    Science.gov (United States)

    Zhang, Jingdong; Zhu, Guoyi; Wang, Erkang

    1996-08-01

    In situ electrochemical scanning tunneling microscopy (ECSTM) has been employed to follow the renewal process of a graphite electrode accompanied by flavin adenine dinucleotide (FAD) electrochemical reaction which involves adsorption of the reduced form (FADH 2) and desorption of the oxidized form (FAD). The renewal process initiates from steps or kinks on the electrode surface, which provide high active sites for adsorption. This renewal depends on the working electrode potential, especially in the range near the FAD redox potential. Our experiment suggests that delamination of the graphite surface is caused by interaction between the substrate and adsorbed molecules. A simple model is proposed to explain this phenomenon.

  3. Umbilicoplasty for large protruding umbilicus accompanying umbilical hernia: a simple and effective technique.

    Science.gov (United States)

    Ikeda, Hitoshi; Yamamoto, Hideki; Fujino, Junko; Kisaki, Yoshiyuki; Uchida, Hiroo; Ishimaru, Yuki; Hasumi, Toshiaki; Hamajima, Akito

    2004-02-01

    A large umbilical protrusion with redundant skin accompanying an umbilical hernia sometimes needs umbilicoplasty. Although several different techniques for making umbilical depression have been used, the results of the plastic surgery are sometimes unsatisfactory due to postoperative flattening or disappearance of the umbilical depression. To make a permanent umbilical depression that is cosmetically acceptable, we have modified the techniques. Umbilicoplasty was performed in 14 children whose ages ranged from 6 months-6 years and 3 months (median, 1 year and 10 months) and who had umbilical hernia with a large umbilical protrusion. After the fascial defect was closed, the diameter of the umbilicus was reduced to half that before surgery by removing fan-shaped skin flaps and approximating skin edges, and then inverting the umbilicus and fixing it caudally to the fascia and skin. There were no postoperative complications, and no flattening or disappearance of umbilical depression was observed during the follow-up of 10-19 months. The authors' technique of umbilicoplasty for a large protruding umbilicus accompanying umbilical hernia is a simple method that produces acceptable cosmetic results.

  4. Increased susceptibility to fungal disease accompanies adaptation to drought in Brassica rapa.

    Science.gov (United States)

    O'Hara, Niamh B; Rest, Joshua S; Franks, Steven J

    2016-01-01

    Recent studies have demonstrated adaptive evolutionary responses to climate change, but little is known about how these responses may influence ecological interactions with other organisms, including natural enemies. We used a resurrection experiment in the greenhouse to examine the effect of evolutionary responses to drought on the susceptibility of Brassica rapa plants to a fungal pathogen, Alternaria brassicae. In agreement with previous studies in this population, we found an evolutionary shift to earlier flowering postdrought, which was previously shown to be adaptive. Here, we report the novel finding that postdrought descendant plants were also more susceptible to disease, indicating a rapid evolutionary shift to increased susceptibility. This was accompanied by an evolutionary shift to increased specific leaf area (thinner leaves) following drought. We found that flowering time and disease susceptibility displayed plastic responses to experimental drought treatments, but that this plasticity did not match the direction of evolution, indicating that plastic and evolutionary responses to changes in climate can be opposed. The observed evolutionary shift to increased disease susceptibility accompanying adaptation to drought provides evidence that even if populations can rapidly adapt in response to climate change, evolution in other traits may have ecological effects that could make species more vulnerable.

  5. Measurement of the specific airway resistance by plethysmography in young children accompanied by an adult

    DEFF Research Database (Denmark)

    Klug, B; Bisgaard, H

    1997-01-01

    The purpose of this study was to evaluate a procedure for measurement of specific airway resistance (sRaw) by whole body plethysmography in young awake children accompanied by an adult. sRaw was measured by a single-step procedure, omitting the measurement of the thoracic gas volume. The frequency...... dependency of sRaw was investigated and the accuracy of simulating body temperature, atmospheric pressure and saturation with water vapour (BTPS) conditions by electronic compensation was assessed. One hundred and thirty one children with asthma were studied. In 57 children (mean (SD) age 5.6 (1.8) yrs) who...... performed measurements with and without an accompanying adult, the mean value of sRaw was 1.45 (0.36) and 1.44 (0.38) kPa x s, respectively, with a mean difference of 0.008 (0.152) kPa x s, and mean within-subject coefficients of variations (CV) of 8% and 10%, respectively. In 52 children (mean age 3.3 (0...

  6. Theta burst stimulation in the treatment of incapacitating tinnitus accompanied by severe depression.

    Science.gov (United States)

    Soekadar, Surjo R; Arfeller, Carola; Rilk, Albrecht; Plontke, Stefan K; Plewnia, Christian

    2009-04-01

    This case report describes the use of transcranial magnetic theta burst stimulation (TBS) in the treatment of incapacitating tinnitus accompanied by symptoms of severe depression. Tinnitus is known to be associated with hyperactivity and maladaptive cortical reorganization of the central auditory system. Combined with anxiety and depression, it can occasionally constitute a psychiatric emergency. Recently, it has been demonstrated that tinnitus can be temporarily suppressed by non-invasive transcranial magnetic stimulation. TBS is a newly developed technique for rapid and lasting modulation of cortical excitability. Herein, we present a case of a 54-year-old woman with incapacitating tinnitus that has significantly decreased after three cycles of 1-week treatment with continuous TBS to the temporo-parietal auditory association cortex. According to the Tinnitus Questionnaire, tinnitus intensity decreased from 84 points before to 59 points after treatment. Hamilton Rating Scale for Depression score dropped from 44 to 23 points. TBS showed to be efficient, well-tolerated, and practical in the management of distressing tinnitus accompanied by symptoms of severe depression.

  7. The Harmonic Walk: An Interactive Physical Environment to Learn Tonal Melody Accompaniment

    Directory of Open Access Journals (Sweden)

    Marcella Mandanici

    2016-01-01

    Full Text Available The Harmonic Walk is an interactive physical environment designed for learning and practicing the accompaniment of a tonal melody. Employing a highly innovative multimedia system, the application offers to the user the possibility of getting in touch with some fundamental tonal music features in a very simple and readily available way. Notwithstanding tonal music is very common in our lives, unskilled people as well as music students and even professionals are scarcely conscious of what these features actually are. The Harmonic Walk, through the body movement in space, can provide all these users a live experience of tonal melody structure, chords progressions, melody accompaniment, and improvisation. Enactive knowledge and embodied cognition allow the user to build an inner map of these musical features, which can be acted by moving on the active surface with a simple step. Thorough assessment tests with musicians and nonmusicians high school students could prove the high communicative power and efficiency of the Harmonic Walk application both in improving musical knowledge and in accomplishing complex musical tasks.

  8. Analysis of Pathogen Distribution and Drug Resistance of Nosocomial Infections Accompanied in Patients with Malignant Tumor

    Institute of Scientific and Technical Information of China (English)

    YAO Dong-mei; CAO Wei; QING Zhi-ju

    2008-01-01

    Objective:To investigate the pathogen distribution and drug resistance of nosocomial infections accompanied in patients with malignant tumor.Methods:The pathogen culture and drug-sensitivity data of 107 specimens isolated from malignant tumor patients accompanied with nosocomial infection were retrospectively analyzed.Results:Among 118 strains of pathogens isolated from 107 specimens,77 were gram-negative bacillus(65.3%),26 were gram-positive coccus(65.3%),and 15 were fungus(12.7%).Eleven specimens were revealed to have combined infection of bacterium and fungus.Gram-negative bacillus showed high sensitivity to amikacin,ciprofloxacin,and tienam.Gram-positive cocci were highly sensitive to tienam and vancomycin.The bacteria were resistant to other antibiotics in different degrees.Vancomycin-resistant staphylococcus was not detected.Candida was sensitive to antifungals.Conclusion:Conditional pathogenic bacteria were mainly responsible for nosocomial infections in malignant tumor patients with considerable drug resistance.This shows that bacterial tests and the rational use of antibiotics should be emphasized in clinical practice to prevent the formation of drug resistant strains and further endogenous infections.

  9. Metastatic Crohn's disease accompanying granulomatous vasculitis and lymphangitis in the vulva.

    Science.gov (United States)

    Ishida, Mitsuaki; Iwai, Muneo; Yoshida, Keiko; Kagotani, Akiko; Okabe, Hidetoshi

    2013-01-01

    Metastatic Crohn's disease (CD) is an extremely rare extragastrointestinal manifestation of CD, and is characterized histopathologically by the presence of non-caseating granulomatous inflammation. Granulomatous vasculitis and lymphangitis have rarely been documented in metastatic CD. Herein, we report the first documented case of metastatic CD accompanied by both granulomatous vasculitis and lymphangitis in the vulva. A 35-year-old Japanese female with CD presented with multiple small nodules in her vulva. Biopsy was performed under a clinical diagnosis of genital warts. A histopathological study revealed marked lymphangiectasia in the papillary dermis. Within the dilated lymphatics, lymphocytes and aggregates of macrophages were present, which are typical features of granulomatous lymphangitis. Tiny non-caseating granulomas and granulomatous vasculitis were also observed. Accordingly, a diagnosis of metastatic CD accompanied by both granulomatous vasculitis and lymphangitis was made. The occurrence of cutaneous lesions in patients with CD is well known. Albeit extremely rare, lymphangiectasia has been reported in the vulva of CD patients that clinically mimicked viral warts, as in the present case. The diagnosis of metastatic CD in the present case was not difficult because characteristic histopathological features were present, and a clinical history of CD was available. However, a few cases of genital swelling associated with granulomatous inflammation prior to a diagnosis of gastrointestinal CD have been documented. Therefore, granulomatous vasculitis and lymphangitis in the external genitals should be considered as potential indication of metastatic CD even in cases without a history of gastrointestinal CD.

  10. Bound magnetic exciton polaron accompanied by large lattice displacement and luminescence in EuSe

    Science.gov (United States)

    Umehara, Masakatsu

    1998-08-01

    We have studied the luminescence in EuSe using the photoinduced localized magnetic polaron (PILMP) model previously proposed for EuTe (Phys. Rev. B 52 (1995) 8140). This paper shows the characteristics of the luminescence around 1.47 eV recently reported (J. Phys. Soc. Japan 63 (1994) 4616) for EuSe is consistent with this model. The PILMP is bound to the localized 4f hole that is created in the photoabsorption process, and accompanies a large lattice displacement induced by the 4f hole. By taking into account this large lattice displacement, we can understand the characteristics of the luminescence, such as the emission energy, the Stokes shift and the spectrum width, quantitatively. The existence of the large lattice displacement is a common feature for EuTe and EuSe. On the other hand, the magnetic polaron effect in EuSe is quite large in comparison to that in EuTe, which causes the difference in the temperature dependence of the emission energy and the spectrum width between EuTe and EuSe. Through the analysis of the luminescence in EuSe, we can see a typical magnetic polaron bound to the 4f hole, that is a bound magnetic exciton polaron accompanied by the large lattice displacement.

  11. Acrolein and Human Disease: Untangling the Knotty Exposure Scenarios Accompanying Several Diverse Disorders.

    Science.gov (United States)

    Burcham, Philip C

    2017-01-17

    Acrolein is a highly toxic electrophile that participates in many diseases, yet efforts to delineate its precise mechanistic contributions to specific conditions are complicated by its wide distribution within human environments. This Perspective develops the proposal that due to its mixed status as environmental pollutant, metabolic byproduct, and endotoxicant which forms via ubiquitous pathophysiological processes, many diseases likely involve acrolein released from multiple sources. Although the category boundaries are indistinct, at least four identifiable exposure scenarios are identifiable. First, in some syndromes, such as those accompanying chronic or acute intoxication with smoke, whatever role acrolein plays in disease pathogenesis mainly traces to exogenous sources such as the combustion of tobacco or other organic matter. A second exposure category involves xenobiotics that undergo metabolism within the body to release acrolein. Still other health conditions, however, involve acrolein that forms via several endogenous pathways, some of which are activated upon intoxication with xenobiotics (i.e., Exposure Category 3), while still others accompany direct physical trauma to body tissues (Exposure Category 4). Further complicating efforts to clarify the role of endogenous acrolein in human disease is the likelihood that many such syndromes are complex phenomena that resemble "chemical mixture exposures" by involving multiple toxic substances simultaneously. This Perspective contends that while recent decades have witnessed much progress in describing the deleterious effects of acrolein at the cellular and molecular levels, more work is needed to define the contributions of different acrolein sources to "real-world" health conditions in human subjects.

  12. Influence of accompanying substances of hemp fibres on their electric resistance

    Directory of Open Access Journals (Sweden)

    Pejić Biljana

    2006-01-01

    Full Text Available Hemp fibres belong to the group of natural, cellulose bast fibres. These fibres have exceptional properties such as: antimicrobial effect, absence of allergy effect, extraordinary sorption properties, good electro-physical properties (small static electricity in regard to other cellulose fibres as well as high values of breaking strength (the natural fibre with the highest strength. However, hemp fibres have some defects: heterogeneous chemical composition, large quantity of accompanying substances (lignin pectins, waxes and unsatisfactory fineness and eveness. It is possible to a great extent to eliminate or reduce, the defects of hemp fibres by of appropriate modification treatments. In order to determine the appropriate modification treatment of hemp fibres, the dependences between the chemical composition, fineness and electric resistance of hemp fibres were presented in this paper. In the experimental part of the paper, by the application of a procedure for the determination of the chemical composition, the accompanying supstances of hemp fibres were gradually removed. After each phase some fibrous substrates were separated. After that the fineness and electric resistance were determined. This experiment was conducted in order to define the influence of each component of hemp fibres on the fineness and electric resistance. In this paper, hemp fibres were modified by an aqueous solution of sodium hydroxide, under different conditions of modification. The influence of modification conditions on the fineness and electric resistance were studied.

  13. Case of absence of the septum pellucidum accompanied with hydrocephalus and detected by computerized tomography

    Energy Technology Data Exchange (ETDEWEB)

    Tominaga, K.; Kobayashi, E.; Yamamoto, K.; Asakura, T. (Kagoshima Univ. (Japan). Faculty of Medicine)

    1981-06-01

    A case of absence of the septum pellucidum accompanied with hydrocephalus in a 56 year old woman who had complained headache, dizziness and numbness of left leg was reported. Absence of the septum pellucidum in this case had not been detected until CT scan was performed. Ventriculoperitoneal shunt was performed, then her complaints were cleared up. The discussion was limited as far as possible to absence of the septum pellucidum without agenesis of the corpus callosum with a few references. Encephalographic characteristics of absence of the septum pellucidum were first noted by Foster in 1935. The real prevalence should lie at about 1 : 400 in a sufficiently large encephalographical series, which should be an estimate between 2 and 3 per 100,000 of population. Encephalography, although, afflicts patients with headache, nausea and vomiting, moreover it accompanied with possible intracranial infection. On the other hand, CT scan has cleared up these problems, we can perform it easily in many cases, and know about status of the septum pellucidum. Further examination for more distinct information are taken by coronal section and ''high definition 5 mm slice thickness'' of CT scan.

  14. Should parents accompany pediatric interfacility ground ambulance transports? The parent's perspective.

    Science.gov (United States)

    Woodward, G A; Fleegler, E W

    2000-12-01

    Parental accompaniment can be a positive experience for the parent and the patient, as perceived by the parent. It can also be accomplished effectively without hindering the delivery of intratransport medical care by a nurse/nurse or nurse/physician transport team. This survey, along with the responses from other parents, led our team to adopt the position that a parent is welcome and encouraged to accompany the transport team if he or she wishes to. The team has recognized the importance of the family unit during the stressful period surrounding an acute medical issue and interfacility transport. The transport team reserves the option to ask that a parent not ride along if they suspect the parent might not function as a supportive team member (ie, the parent is belligerent, inebriated, or hostile). The parent normally rides in the passenger seat of the ambulance, and we encourage him or her to interact with the patient as much as possible. Occasionally parents ride in the back of the ambulance if the patient's situation allows for that option (ie, no anticipated need for potential interventions, number of team personnel, etc.).

  15. Seizure-induced damage to substantia nigra and globus pallidus is accompanied by pronounced intra- and extracellular acidosis

    Energy Technology Data Exchange (ETDEWEB)

    Inamura, K.; Smith, M.L.; Hansen, A.J.; Siesjoe, B.K. (Univ. of Lund (Sweden))

    1989-12-01

    Status epilepticus of greater than 30-min duration in rats gives rise to a conspicuous lesion in the substantia nigra pars reticulata (SNPR) and globus pallidus (GP). The objective of the present study was to explore whether the lesion, which encompasses necrosis of both neurons and glial cells, is related to intra- and extracellular acidosis. Using the flurothyl model previously described to produce seizures, we assessed regional pH values with the autoradiographic 5,5-dimethyl(2-14C)oxazolidine-2,4-dione technique. Regional pH values were assessed in animals with continuous seizures for 20 and 60 min, as well as in those allowed to recover for 30 and 120 min after seizure periods of 20 or 60 min. In additional animals, changes in extracellular fluid pH (pHe) were measured with ion-selective microelectrodes, and extracellular fluid (ECF) volume was calculated from the diffusion profile for electrophoretically administered tetramethylammonium. In structures such as the neocortex and the hippocampus, which show intense metabolic activation during seizures, status epilepticus of 20- and 60-min duration was accompanied by a reduction of the composite tissue pH (pHt) of 0.2-0.3 unit. Recovery of pHt was observed upon termination of seizures. In SNPR and in GP, the acidosis was marked to excessive after 20 and 60 min of seizures (delta pHt approximately 0.6 after 60 min).

  16. Membrane partitioning of anionic, ligand-coated nanoparticles is accompanied by ligand snorkeling, local disordering, and cholesterol depletion.

    Directory of Open Access Journals (Sweden)

    Paraskevi Gkeka

    2014-12-01

    Full Text Available Intracellular uptake of nanoparticles (NPs may induce phase transitions, restructuring, stretching, or even complete disruption of the cell membrane. Therefore, NP cytotoxicity assessment requires a thorough understanding of the mechanisms by which these engineered nanostructures interact with the cell membrane. In this study, extensive Coarse-Grained Molecular Dynamics (MD simulations are performed to investigate the partitioning of an anionic, ligand-decorated NP in model membranes containing dipalmitoylphosphatidylcholine (DPPC phospholipids and different concentrations of cholesterol. Spontaneous fusion and translocation of the anionic NP is not observed in any of the 10-µs unbiased MD simulations, indicating that longer timescales may be required for such phenomena to occur. This picture is supported by the free energy analysis, revealing a considerable free energy barrier for NP translocation across the lipid bilayer. 5-µs unbiased MD simulations with the NP inserted in the bilayer core reveal that the hydrophobic and hydrophilic ligands of the NP surface rearrange to form optimal contacts with the lipid bilayer, leading to the so-called snorkeling effect. Inside cholesterol-containing bilayers, the NP induces rearrangement of the structure of the lipid bilayer in its vicinity from the liquid-ordered to the liquid phase spanning a distance almost twice its core radius (8-10 nm. Based on the physical insights obtained in this study, we propose a mechanism of cellular anionic NP partitioning, which requires structural rearrangements of both the NP and the bilayer, and conclude that the translocation of anionic NPs through cholesterol-rich membranes must be accompanied by formation of cholesterol-lean regions in the proximity of NPs.

  17. Membrane partitioning of anionic, ligand-coated nanoparticles is accompanied by ligand snorkeling, local disordering, and cholesterol depletion.

    Science.gov (United States)

    Gkeka, Paraskevi; Angelikopoulos, Panagiotis; Sarkisov, Lev; Cournia, Zoe

    2014-12-01

    Intracellular uptake of nanoparticles (NPs) may induce phase transitions, restructuring, stretching, or even complete disruption of the cell membrane. Therefore, NP cytotoxicity assessment requires a thorough understanding of the mechanisms by which these engineered nanostructures interact with the cell membrane. In this study, extensive Coarse-Grained Molecular Dynamics (MD) simulations are performed to investigate the partitioning of an anionic, ligand-decorated NP in model membranes containing dipalmitoylphosphatidylcholine (DPPC) phospholipids and different concentrations of cholesterol. Spontaneous fusion and translocation of the anionic NP is not observed in any of the 10-µs unbiased MD simulations, indicating that longer timescales may be required for such phenomena to occur. This picture is supported by the free energy analysis, revealing a considerable free energy barrier for NP translocation across the lipid bilayer. 5-µs unbiased MD simulations with the NP inserted in the bilayer core reveal that the hydrophobic and hydrophilic ligands of the NP surface rearrange to form optimal contacts with the lipid bilayer, leading to the so-called snorkeling effect. Inside cholesterol-containing bilayers, the NP induces rearrangement of the structure of the lipid bilayer in its vicinity from the liquid-ordered to the liquid phase spanning a distance almost twice its core radius (8-10 nm). Based on the physical insights obtained in this study, we propose a mechanism of cellular anionic NP partitioning, which requires structural rearrangements of both the NP and the bilayer, and conclude that the translocation of anionic NPs through cholesterol-rich membranes must be accompanied by formation of cholesterol-lean regions in the proximity of NPs.

  18. Effects of voice timbre and accompaniment on working memory as measured by sequential monosyllabic digit recall performance.

    Science.gov (United States)

    Silverman, Michael J; Schwartzberg, Edward T

    2014-01-01

    Information is often paired with music in an attempt to facilitate recall and enhance learning. However, there is a lack of basic research investigating how music carrying information might facilitate recall and subsequent learning. The purpose of the current study was to determine the effects of voice timbre and accompaniment on working memory as measured by recall performance on a sequential digit recall task. Specific research questions were as follows: (a) How might female and male voice timbres affect serial recall? (b) How might piano, guitar, and no accompaniment affect serial recall? (c) Do music majors have enhanced recall accuracy when compared to nonmusic majors? The recall of information paired with six different melodies was tested on 60 university students. Melodies were composed and recorded using female and male voices with three levels of accompaniment: guitar, piano, and no accompaniment. Participants had more accurate recall during the male voice and piano and no accompaniment conditions and least accurate recall during the female voice and guitar accompaniment conditions. As participants had most accurate recall during the male voice and with piano or no accompaniment, clinicians are encouraged to consider using no accompaniment or piano accompaniment when initially teaching social and academic information paired with music for later recall. When possible, vocal timbre (i.e., the potential benefit of male voicing) should also be considered. Implications for clinical practice, limitations of the study, and suggestions for future research are provided. © the American Music Therapy Association 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  19. Comprehensive evaluation of functional and anatomical disorders of the patients with distal occlusion and accompanying obstructive sleep apnea syndrome

    Science.gov (United States)

    Nabiev, F. H.; Dobrodeev, A. S.; Libin, P. V.; Kotov, I. I.; Ovsyannikov, A. G.

    2015-11-01

    The paper defines the therapeutic and rehabilitation approach to the patients with Angle's classification Class II dento-facial anomalies, accompanied by obstructive sleep apnea (OSA). The proposed comprehensive approach to the diagnostics and treatment of patients with posterior occlusion, accompanied by OSA, allows for objective evaluation of intensity of a dento-facial anomaly and accompanying respiratory disorders in the nasal and oral pharynx, which allows for the pathophysiological mechanisms of OSA to be identified, and an optimal plan for surgical procedures to be developed. The proposed comprehensive approach to the diagnostics and treatment of patients with Angle's classification Class II dento-facial anomalies provides high functional and aesthetic results.

  20. Student Solutions Manual to accompany Fundamentals of Physics,7th Edition

    Science.gov (United States)

    Halliday, David; Resnick, Robert; Walker, Jearl; Christman, J. Richard

    2004-06-01

    Work more effectively and check solutions as you go along with the text! This Student Solutions Manual that accompanies Fundamentals of Physics, 7th Edition, provides readers with complete, worked-out solutions to 30% of the end-of-chapter problems. These problems are indicated in the text by an ssm icon. No other book on the market today can match the 30-year success of Halliday, Resnick and Walker's Fundamentals of Physics! In a breezy, easy-to-understand style this Seventh Edition offers a solid understanding of fundamental physics concepts, and helps readers apply this conceptual understanding to quantitative problem solving. This book offers a unique combination of authoritative content and stimulating applications.

  1. Nonunion of the First Sternocostal Synchondrosis Accompanied by Sternoclavicular Joint Synovitis

    Directory of Open Access Journals (Sweden)

    Makoto Takeuchi

    2014-01-01

    Full Text Available Injury to the sternocostal synchondrosis of the first rib is quite rare. We report one such case in a 50-year-old man with nonunion of the first sternocostal synchondrosis accompanied by synovitis of the sternoclavicular joint. He first underwent arthroscopic surgery of the left sternoclavicular joint. Postoperatively, the patient’s symptoms decreased by half, but another pain and crepitus at the inferior lateral portion of the sternoclavicular joint developed. Since MRI and functional CT reexaminations revealed nonunion of the first sternocostal synchondrosis, resection arthroplasty of the first sternocostal joint was performed. This resulted in immediate resolution of the symptoms. At 2-year follow-up, his symptoms disappeared entirely with no limited range of motion of the shoulder.

  2. Cannon Fire Soon to Accompany Bugle Call at Fort Detrick | Poster

    Science.gov (United States)

    Beginning June 14, the familiar bugle calls at Fort Detrick will be joined by a special percussion instrument: a cannon. A single cannon shot will be fired on the first note of “Reveille,” which signals the start of each day and is accompanied by the raising of the American flag. “Reveille” sounds at 6:30 a.m. At 5 p.m., Fort Detrick plays “Retreat,” which alerts the post that the flag is about to be lowered. The cannon will fire on the last note of “Retreat.” After a five-second pause, “To the Color” plays as the flag is lowered.

  3. Adult onset Still's disease accompanied by acute respiratory distress syndrome: A case report.

    Science.gov (United States)

    Xi, Xiao-Tu; Wang, Mao-Jie; Huang, Run-Yue; Ding, Bang-Han

    2016-09-01

    Adult onset Still's disease (AOSD) is a systemic inflammatory disorder characterized by rash, leukocytosis, fever and arthralgia/arthritis. The most common pulmonary manifestations associated with AOSD are pulmonary infiltrates and pleural effusion. The present study describes a 40-year-old male with AOSD who developed fever, sore throat and shortness of breath. Difficulty breathing promptly developed, and the patient was diagnosed with acute respiratory distress syndrome (ARDS). The patient did not respond to antibiotics, including imipenem, vancomycin, fluconazole, moxifloxacin, penicillin, doxycycline and meropenem, but was sensitive to glucocorticoid treatment, including methylprednisolone sodium succinate. ARDS accompanied by AOSD has been rarely reported in the literature. In conclusion, in a patient with ARDS who does not respond to antibiotic treatment, the involvement of AOSD should be considered.

  4. Anaesthesia management for acute appendicitis in cases with Sjogren's syndrome accompanying autoimmune hepatitis.

    Science.gov (United States)

    Demirel, Ismail; Ozer, Ayse Belin; Bayar, Mustafa K; Erhan, Omer L

    2013-04-30

    Characterised by lymphocytic infiltration of exocrine glands, Sjögren's syndrome (SS) is a chronic autoimmune disease. Symptoms belonging to the involved systems may occur owing to the fact that it affects multiple systems. While rheumatoid arthritis is observed concomitantly, its co-occurrence with autoimmune hepatitis is astonishingly common. Through this case report, we intended to review issues that should be attended to while administering anaesthesia to a patient with SS accompanying autoimmune hepatitis. In the light of literature, we aimed to discuss anaesthesia management to the patient with SS and issues stemming from the clinical features of SS. In SS, the expected issues are liver problems related autoimmune hepatitis and respiratory problems related pulmonary fibrosis. A careful preoperative evaluation, a comprehensive preparation against difficulty in intubation, a selective anaesthesia management in terms of autoimmune hepatitis and close monitoring of postoperative respiration may prevent or decrease possible complications.

  5. Evidence for coral range expansion accompanied by reduced diversity of Symbiodinium genotypes

    Science.gov (United States)

    Grupstra, Carsten G. B.; Coma, Rafel; Ribes, Marta; Leydet, Karine Posbic; Parkinson, John Everett; McDonald, Kelly; Catllà, Marc; Voolstra, Christian R.; Hellberg, Michael E.; Coffroth, Mary Alice

    2017-09-01

    Zooxanthellate corals are threatened by climate change but may be able to escape increasing temperatures by colonizing higher latitudes. To determine the effect of host range expansion on symbiont genetic diversity, we examined genetic variation among populations of Symbiodinium psygmophilum associated with Oculina patagonica, a range-expanding coral that acquires its symbionts through horizontal transmission. We optimized five microsatellite primer pairs for S. psygmophilum and tested them on Oculina spp. samples from the western North Atlantic and the Mediterranean. We then used them to compare symbiont genotype diversity between an Iberian core and an expansion front population of O. patagonica. Only one multilocus S. psygmophilum genotype was identified at the expansion front, and it was shared with the core population, which harbored seven multilocus genotypes. This pattern suggests that O. patagonica range expansion is accompanied by reduced symbiont genetic diversity, possibly due to limited dispersal of symbionts or local selection.

  6. EVALUATION OF INFLAMMATORY CHANGES IN JOINTS AT PATIENTS WITH HEMOPHILIA ACCOMPANIED RECURRENT HEMARTHROSIS

    Directory of Open Access Journals (Sweden)

    I. L. Davydkin

    2012-01-01

    Full Text Available Objective: to search for new diagnostic approaches for estimating the prevalence of posthemorrhagic phase of inflammation in the joints of patients with hemophilia accompanied recurrent hemarthrosis. Using the method of computerized infrared thermography measured the temperature above the knee, ankle and lower leg. Analysis of the data held with a specially developed algorithm that forms the basis of a computer program for computer (2009. It is established that with increasing temperature over the joint at high risk of recurrence monthly hemarthrosis. If the joint hemarthrosis was not, then an increase in temperature may indicate a latent flowing inflammation. Assessing the prevalence of inflammation in the joints of patients with hemophilia has prognostic value: widespread inflammation is characterized by continuous recurrent course, for a limited - monthly recurrent hemarthrosis, in the absence of inflammation in the joint, as evidenced by normal or low-grade fever, recurrent hemarthrosis are likely rare.

  7. [The efficacy and safety of terazosin and tamsulosin in patients with urinary disturbance accompanying prostatic hypertrophy].

    Science.gov (United States)

    Suzuki, Y; Katoh, T; Isurugi, K; Obara, W; Omori, S; Goto, Y; Fujioka, T; Numasato, S

    2001-01-01

    Terazosin (TE) and tamsulosin (TA) were allocated randomly to 38 patients who had urinary disturbance accompanying prostatic hypertrophy, and the efficacy and safety of the drugs were examined. Subjective symptoms due to I-PSS were improved significantly in both TE and TA groups. On the other hand, objective symptoms such as the maximum urinary flow and mean urinary flow were improved more in the TE group. TE showed hypotensive and cholesterol-decreasing effects in patients who also had hypertension and hyperlipemia. No unknown adverse reactions were observed in either groups, and the drugs were shown to be highly safe. TE was considered to be useful as the first choice drug for the patients with hypertension and or hyperlipemia and those with severe objective symptoms. TA was considered to be useful for the patients with impaired drug compliance or those with severe subjective symptoms though objective symptoms were not so severe.

  8. DNA barcoding should accompany taxonomy - the case of Cerebratulus spp (Nemertea).

    Science.gov (United States)

    Sundberg, P; Thuroczy Vodoti, E; Strand, M

    2010-03-01

    Many issues in DNA barcoding need to be solved before it can reach its goal to become a general database for species identification. While species delimitations are more or less well established in several taxa, there are still many groups where this is not the case. Without the proper taxonomic background/knowledge and corroboration with other kinds of data, the DNA barcoding approach may fail to identify species accurately. The classification and taxonomy of phylum Nemertea (nemerteans, ribbon worms) are traditionally based on morphology, but are not corroborated by an increasing amount of genetic data when it comes to classification either into species or into higher taxa. The taxonomy of the phylum needs to be improved before the full potential of DNA barcoding can be utilized to make sure that valid Linnean names accompany the barcode sequences. We illustrate the problematic situation in the phylum Nemertea by a case study from the genus Cerebratulus. © 2009 Blackwell Publishing Ltd.

  9. Evidence for coral range expansion accompanied by reduced diversity of Symbiodinium genotypes

    KAUST Repository

    Grupstra, Carsten G. B.

    2017-05-15

    Zooxanthellate corals are threatened by climate change but may be able to escape increasing temperatures by colonizing higher latitudes. To determine the effect of host range expansion on symbiont genetic diversity, we examined genetic variation among populations of Symbiodinium psygmophilum associated with Oculina patagonica, a range-expanding coral that acquires its symbionts through horizontal transmission. We optimized five microsatellite primer pairs for S. psygmophilum and tested them on Oculina spp. samples from the western North Atlantic and the Mediterranean. We then used them to compare symbiont genotype diversity between an Iberian core and an expansion front population of O. patagonica. Only one multilocus S. psygmophilum genotype was identified at the expansion front, and it was shared with the core population, which harbored seven multilocus genotypes. This pattern suggests that O. patagonica range expansion is accompanied by reduced symbiont genetic diversity, possibly due to limited dispersal of symbionts or local selection.

  10. Theory of the Jitter radiation in a magnetized plasma accompanying temperature gradient

    CERN Document Server

    Hattori, Makoto

    2016-01-01

    The linear stability of a magnetized plasma accompanying temperature gradient was reexamined by using plasma kinetic theory. The anisotropic velocity distribution function was decomposed into two components. One is proportional to the temperature gradient parallel to and the other is proportional to the temperature gradient perpendicular to the back ground magnetic field. Since the amplitude of the anisotropic velocity distribution function is proportional to the heat conductivity and the heat conductivities perpendicular to the magnetic field is strongly reduced, the first component of the anisotropic velocity distribution function is predominant. The anisotropic velocity distribution function induced by the temperature gradient along the back ground magnetic field drives plasma kinetic instability and the circular polarized magnetic plasma waves are excited. The instability is almost identical to Weibel instability in weakly magnetized plasma. However, depending on whether wave vectors of modes are parallel...

  11. The impact of foreign postings on accompanying military spouses: an ethnographic study

    Directory of Open Access Journals (Sweden)

    Gillian Blakely

    2014-08-01

    Full Text Available As part of an ethnographic study, the impact of foreign postings on spouses who accompany military personnel was explored. Individual interviews and focus groups with 34 British military spouses based in one location in southern Europe were conducted. Key findings suggested that reaction to a foreign posting was a reflection of personal attitudes, prior experiences, support, ability to adjust to change and strength of relationship with the serving spouse and community. For many the experience was positive due to the increased opportunity for family time, for others this helped to compensate for the difficulties experienced. Some military spouses experienced significant distress on the posting, particularly if the family was not well-supported. The potential implications of military spouses not adapting to foreign postings have significant implications for healthcare practice. Provision of more appropriate support resources before and during the posting would facilitate the transition for the military spouse and their family.

  12. Cannon Fire Soon to Accompany Bugle Call at Fort Detrick | Poster

    Science.gov (United States)

    Beginning June 14, the familiar bugle calls at Fort Detrick will be joined by a special percussion instrument: a cannon. A single cannon shot will be fired on the first note of “Reveille,” which signals the start of each day and is accompanied by the raising of the American flag. “Reveille” sounds at 6:30 a.m. At 5 p.m., Fort Detrick plays “Retreat,” which alerts the post that the flag is about to be lowered. The cannon will fire on the last note of “Retreat.” After a five-second pause, “To the Color” plays as the flag is lowered.

  13. Quotidian of accompanying family members in an environment of care: the emergence of hospital tribes.

    Science.gov (United States)

    Passos, Silvia da Silva Santos; Pereira, Álvaro; Nitschke, Rosane Gonçalves

    2016-01-01

    Compreender o relacionamento no quotidiano dos familiares acompanhantes nos cenários de cuidado que se aproximam da metáfora da tribo no ambiente hospitalar. Estudo qualitativo com dados coletados a partir de entrevistas semiestruturadas e observação com 16 familiares acompanhantes de pessoas hospitalizadas com dependência para o autocuidado. Os dados foram submetidos à análise temática e analisados através da metáfora da tribo proposta pela sociologia compreensiva. Os familiares formam um agrupamento social em torno do cuidado onde encontramos as características das tribos: a ambiência emocional; a solidariedade baseada nos vínculos de simpatia e ajuda mútua; a nebulosa afetual no processo interacional; a lógica da fusão nas relações tácteis e a comunhão/religiosidade no processo de ligação numa identidade coletiva. Os familiares na presença do trágico criam agrupamentos sociais que se assemelham a tribos cujo totem é o cuidado. Understand the quotidian relationships of accompanying family members in an environment of care, which are close to the metaphor of a tribe in hospital environment. METHODQualitative study with data gathered from semi-structured interviews and observations with 16 family members accompanying hospitalized individuals with dependence on self-care. Data were submitted to thematic analysis, and analyzed through the metaphor of "tribe" proposed by comprehensive sociology. Family members build up social clusters around caring, where we find traits typical of tribes: emotional ambience; solidarity based on links of sympathy and mutual assistance; an affectual nebula in the process of interaction; a logic of fusion in tactile relations; and communion/religiosity in the process of connecting in a collective identity. In the presence of tragedy, families build social clusters similar to tribes having care as a totem.

  14. The role of semantic abstractness and perceptual category in processing speech accompanied by gestures

    Directory of Open Access Journals (Sweden)

    Arne eNagels

    2013-12-01

    Full Text Available Space and shape are distinct perceptual categories. In language, perceptual information can also be used to describe abstract semantic concepts like a ‘rising income’ (space or a ‘square personality’ (shape. Despite being inherently concrete, co-speech gestures depicting space and shape can accompany concrete or abstract utterances. Here, we investigated the way that abstractness influences the neural processing of the perceptual categories of space and shape in gestures. Thus, we tested the hypothesis that the neural processing of perceptual categories is highly dependent on language context.In a two-factorial design, we investigated the neural basis for the processing of gestures containing shape (SH and spatial information (SP when accompanying concrete (c or abstract (a verbal utterances. During fMRI data acquisition participants were presented with short video clips of the four conditions (cSP, aSP, cSH, aSH while performing an independent control task.Abstract (a as opposed to concrete (c utterances activated temporal lobes bilaterally and the left inferior frontal gyrus (IFG for both shape-related (SH and space-related (SP utterances. An interaction of perceptual category and semantic abstractness in a more anterior part of the left IFG and inferior part of the posterior temporal lobe indicates that abstractness strongly influenced the neural processing of space and shape information. Despite the concrete visual input of co-speech gestures in all conditions, space and shape information is processed differently depending on the semantic abstractness of its linguistic context.

  15. Effects of Herbal Acupuncture(Soyeum on Cancer Patients Accompanied by Pain

    Directory of Open Access Journals (Sweden)

    Hwa-Seung Yoo

    2003-02-01

    Full Text Available Objectives : This study was designed to evaluate effects of "Soyeum" on cancer patients accompanied by pain. Materials and Methods : We retrospectively analyzed the medical records of 9 patients accompanied by pain who had been injected with "Soyeum" for over 14 days continuously in East-West Cancer Center of Oriental Hospital of Daejeon University from June 2002 through August 2002. Results : The statistical significance between the pre-treatment and post-treatment results (Changes of Cytokine Level, QOL, BPQ and Pain relief after pain management were analyzed. Analysis of cytokines (IL-12, IFN-γ level showed that the percentage of increase of IL-12 is 60.0%, IFN-γ is 60.0%. Analysis of QOL showed that the percentage of maintenance and improvement is 77.8%. 55.5% of the patients reported a "worst pain" intensity score of 3 or greater, 44.5% reported a "least pain" intensity score of 2 or greater, and 66.7% reported "average pain" intensity score of 2 or greater. 33.3% of the patients were in pain at the time of interview and 22.2% had a current intensity score of 2 or greater. Analysis of pain relief after pain management showed that the percentage of pain relief score of 2 and 3 is 55.6%. The data was expressed as Mean±SE by using descriptive statistics. Statistical significance examined by using paired t-test. Conclusions : It is suggested that "Soyeum" has effects on pain of cancer patients, also expected that "Soyeum" is useful to improve immunoactivity and for cancer patients.

  16. Segmental arterial mediolysis accompanied by renal infarction and pancreatic enlargement: a case report

    Directory of Open Access Journals (Sweden)

    Ito Nobuhisa

    2012-09-01

    Full Text Available Abstract Introduction Due to recent advances in imaging diagnostic techniques, there are an increasing number of case reports of segmental arterial mediolysis. However, there are only a limited number of reports on segmental arterial mediolysis-related abnormalities of abdominal organs other than the intestine. This report describes a case of segmental arterial mediolysis accompanied by abnormalities of abdominal organs without clinical symptoms. Case presentation A 52-year-old Japanese man with hematuria and no prior medical history was referred to a urologist and was diagnosed as having urinary bladder cancer. He underwent trans-urethral resection of the bladder tumor and intra-vesical instillation therapy, which was followed by observation. During follow-up, although no abdominal symptoms were observed, an abdominal computed tomography scan revealed a dissection of the superior mesenteric artery. A false lumen partially occluded by a thrombus was located distal to this occlusion. The lumen was irregularly shaped with narrow and wide sections. Similar irregularities were also observed in the wall of the inferior mesenteric artery. Arterial dissection with thromboembolism in the left renal artery and renal infarction was also observed. Follow-up computed tomography after two months revealed an enlargement of the pancreatic tail adjacent to the splenic artery. Follow-up three-dimensional computed tomography showed gradual re-expansion of the true lumen of the superior mesenteric artery, improvement in arterial wall irregularities, and a reduction in the pancreas enlargement and renal infarction. Over the following 15 months, these changes gradually normalized. On the basis of the vascular changes in multiple arterial systems that resolved spontaneously, we considered that the lesions were associated with segmental arterial mediolysis. Conclusions We present a rare case of segmental arterial mediolysis accompanied by abnormalities of abdominal

  17. Temporal modulations of agonist and antagonist muscle activities accompanying improved performance of ballistic movements.

    Science.gov (United States)

    Liang, Nan; Yamashita, Takamasa; Ni, Zhen; Takahashi, Makoto; Murakami, Tsuneji; Yahagi, Susumu; Kasai, Tatsuya

    2008-02-01

    Although many studies have examined performance improvements of ballistic movement through practice, it is still unclear how performance advances while maintaining maximum velocity, and how the accompanying triphasic electromyographic (EMG) activity is modified. The present study focused on the changes in triphasic EMG activity, i.e., the first agonist burst (AG1), the second agonist burst (AG2), and the antagonist burst (ANT), that accompanied decreases in movement time and error. Twelve healthy volunteers performed 100 ballistic wrist flexion movements in ten 10-trial sessions under the instruction to "maintain maximum velocity throughout the experiment and to stop the limb at the target as fast and accurately as possible". Kinematic parameters (position and velocity) and triphasic EMG activities from the agonist (flexor carpi radialis) and antagonist (extensor carpi radialis) muscles were recorded. Comparison of the results obtained from the first and the last 10 trials, revealed that movement time, movement error, and variability of amplitudes reduced with practice, and that maximum velocity and time to maximum velocity remained constant. EMG activities showed that AG1 and AG2 durations were reduced, whereas ANT duration did not change. Additionally, ANT and AG2 latencies were reduced. Integrated EMG of AG1 was significantly reduced as well. Analysis of the alpha angle (an index of the rate of recruitment of the motoneurons) showed that there was no change in either AG1 or AG2. Correlation analysis of alpha angles between these two bursts further revealed that the close relationship of AG1 and AG2 was kept constant through practice. These findings led to the conclusion that performance improvement in ballistic movement is mainly due to the temporal modulations of agonist and antagonist muscle activities when maximum velocity is kept constant. Presumably, a specific strategy is consistently applied during practice.

  18. Chicken spermatogenesis is accompanied by a genomic-wide loss of DNA methylation.

    Science.gov (United States)

    Rocamora, N; Mezquita, C

    1989-04-24

    Renaturation kinetics of DNA obtained from chicken testis cell nuclei separated by sedimentation at unit gravity showed that the undermethylation, previously observed in meiotic and postmeiotic cells, is not a peculiarity of repetitive sequences, but is also a feature of unique sequences. The large proportion of slowly renaturing, intermediately renaturing and rapidly renaturing DNAs contain 27, 32 and 31% less methylcytosines in meiotic and postmeiotic cells than the corresponding fractions of premeiotic cells. DNA methyltransferase activity is lower in meiotic cells containing undermethylated DNA than in immature testis, enriched in spermatogonia, with higher levels of DNA methylation.

  19. Reducing the Risk of Damage to Power Transformers of 110 kV and Above Accompanying Internal Short Circuits

    Energy Technology Data Exchange (ETDEWEB)

    L’vova, M. M. [JSC “R& D Center at Federal Grid Company of the Unified Power System” (Russian Federation); L’vov, S. Yu. [Presselektro LLC (Russian Federation); Komarov, V. B. [IPCE RAS (Russian Federation); Lyut’ko, E. O. [JSC “R& D Center at Federal Grid Company of the Unified Power System” (Russian Federation); Vdoviko, V. P. [EMA Ltd. (Russian Federation); Demchenko, V. V. [JSC “Boguchanskaya HPP” (Russian Federation); Belyaev, S. G. [PKF Konif Ltd. (Russian Federation); Savel’ev, V. A. [Ivanovo State Power University (Russian Federation); L’vov, M. Yu., E-mail: timashova@nte-power.ru; L’vov, Yu. N. [JSC “R& D Center at Federal Grid Company of the Unified Power System” (Russian Federation)

    2015-03-15

    Methods of increasing the operating reliability of power transformers, autotransformers and shunting reactors in order to reduce the risk of damage, which accompany internal short circuits and equipment fires and explosions, are considered.

  20. Efficacy and safety of exogenous melatonin for secondary sleep disorders and sleep disorders accompanying sleep restriction: meta-analysis

    OpenAIRE

    2006-01-01

    Objective To conduct a systematic review of the efficacy and safety of exogenous melatonin in managing secondary sleep disorders and sleep disorders accompanying sleep restriction, such as jet lag and shiftwork disorder.

  1. Effect of a virtual reality exercise program accompanied by cognitive tasks on the balance and gait of stroke patients

    OpenAIRE

    Lee, In-Wook; Kim, Yong-Nam; Lee, Dong-Kyu

    2015-01-01

    [Purpose] This study aimed to assess the effect of a virtual reality exercise program accompanied by cognitive tasks on the balance and gait of stroke patients. [Subjects] Twenty stroke patients were randomly assigned to two groups 10 to an experimental group that performed a virtual reality exercise program accompanied by cognitive tasks and 10 to a control group. The control group performed a proprioceptive neuromuscular facilitation exercise program. Balance was measured with the Berg Bala...

  2. Initial phases of explosion earthquakes accompanying Vulcanian eruptions at Lokon-Empung volcano, Indonesia

    Science.gov (United States)

    Yamada, Taishi; Aoyama, Hiroshi; Nishimura, Takeshi; Yakiwara, Hiroshi; Nakamichi, Haruhisa; Oikawa, Jun; Iguchi, Masato; Hendrasto, Muhamad; Suparman, Yasa

    2016-11-01

    We examine the initial phases of explosion earthquakes accompanying Vulcanian eruptions at Lokon-Empung volcano in Indonesia to reveal the triggering process of explosive eruptions. In 2012-2013, 56 Vulcanian eruptions at Lokon-Empung were observed by our temporary observation network being comprised of four broadband seismometers and two infrasound microphones at 1.6-6.8 km from the active vent. The seismic records of each explosion earthquake share almost the same waveform characteristics of initial phases, consisting of a small compressional onset (P phase) and a subsequent large dilatational phase (D phase). Particle orbits of both phases show straight motion from beneath the active vent, which suggests that these phases are composed of a longitudinal body wave. For each explosion, the origin times of the P phase precedes 0.8-2.5 s before the occurrence of an explosion at the vent that are detected by infrasound data. Since the signal-to-noise ratio of the P phase is insufficient for a quantitative analysis, we analyze the D phase dominating the initial phases. Our analysis for the signals of 0.2-1.0 Hz shows the D phase are well explained by a cylindrical contraction source with a half-cosine shaped time function located at 1.0-1.3 km depth beneath the active vent. We also recognize that some explosions are followed by a prominent tremor that coincides with continuous ash emission (ET). The seismic amplitudes and intensity of the D phase of events in ET are larger than the explosions without accompanying tremor (EX). The frequency distribution of the time interval from the previous eruption is also different in the events in ET and EX. The implosion source in the initial phases of explosion earthquakes at several km beneath the active vent has been reported at Sakurajima volcano. Since our result shows considerable agreement with the previous works at Sakurajima, both Lokon-Empung and Sakurajima may share similar initial processes of Vulcanian eruptions.

  3. Evaluation of thermal water in patients with functional dyspepsia and irritable bowel syndrome accompanying constipation

    Institute of Scientific and Technical Information of China (English)

    Giovanni Gasbarrini; Marcello Candelli; RiccardoGiuseppe Graziosetto; Sergio Coccheri; Ferdinando Di Iorio; Giuseppe Nappi

    2006-01-01

    AIM: To evaluate the efficacy of water supplementation treatment in patients with functional dyspepsia or irritable bowe syndrome (IBS) accompanying predominant constipation.METHODS: A total of 3 872 patients with functional dyspepsia and 3609 patients with irritable bowel syndrome were enrolled in the study by 18 Italina thermal centres.Patients underwent a first cycle of thermal therapy for 21 d. A year later patients were re-evaluated at the same centre and received another cycle of thermal therapy.A questionnare to collect personal data on social and occupational status, family and pathological case history,life style, clinical records, utilisation of welfare and health structure and devices was administered to each patient at basal time and one year after each thermal treatment.Sixty patients with functional dyspepsia and 20 with IBS and 80 healthy controls received an evaluation of gastric output and oro-cecal transit time by breath test analysis.Breath test was performed at basal time and after water supplementaton therapies. Gastrointestinal symptoms were evaluated at the same time points. Breath samples were analyzed with a mass spectometer and a gascromatograph. Results were expressed as T1/2 and T-lag for octanoic acid breath test and as oro-cecal transit time for lactulose breath test.RESULTS: A significant reduction of prevalence of symptoms was observed at the end of the first and second cycles of thermal therapy in dyspeptic and IBS patients.The analysis of variance showed a real and persistant improvement of symptoms in all patients. After water supplementation for 3 wk a reduction of gastric output was observed in 49 (87.5%) of 56 dyspeptic patients.Both T1/2 and T-lag were significantly reduced after the therapy compared to basal values [91± 12 (T1/2) and 53 ± 11 (T-lag), Tables 1 and 2] with results of octanoic acid breath test similar to healthy subjects. After water supplementation for 3 wk oro-cecal transit time was shorter than that at the

  4. [A case of Guillain-Barré syndrome accompanied by syndrome of inappropriate secretion of antidiuretic hormone].

    Science.gov (United States)

    Inoue, Manabu; Kojima, Yasuhiro; Shirakashi, Yoshitomo; Kanda, Masutarou; Shibasaki, Hiroshi

    2010-10-01

    A 73-year-old Japanese male was admitted because of difficulty in standing up after acute upper respiratory inflammation with mild fever followed by watery diarrhea. Neurological examination revealed moderate proximal muscle weakness and loss of tendon reflexes in all extremities. The blood sodium level was 106 mEq/l on admission. The blood level of antidiuretic hormone (ADH), renin and aldsterone was 11.3 pg/ml (normal value 0.3-4.2), 0.2 ng/ml/h (0.2-2.7) and less than 10.0 pg/ml (38.9-307.0), respectively. The plasma osmolarity was 221 mOsm/kg (270-295), and the urine osmolarity was 416 mOsm/kg (50-1400). EMG and nerve conduction studies suggested acute demyelination in the motor and sensory nerves. CSF revealed 10 cells/mm3 and elevated protein to 98 mg/dl. The clinical course, laboratory data and electrophysiological findings suggested coexistence of the syndrome of inappropriate secretion of ADH (SIADH) and Guillain-Barré syndrome (GBS) from the very early clinical stage of the diseases. The clinical and laboratory findings improved after intravenous administration of saline over three weeks. When GBS is associated with SIADH, hyponatremia is commonly seen at the peak of motor paralysis, often accompanied by autonomic or respiratory failure requiring mechanical ventilation. This was not the case in the present patient. It is postulated that SIADH, like GBS, might be caused by an autoimmune mechanism.

  5. Training shortens search times in children with visual impairment accompanied by nystagmus

    Directory of Open Access Journals (Sweden)

    Bianca eHuurneman

    2014-09-01

    Full Text Available Perceptual learning (PL can improve near visual acuity in 4-9 year old children with visual impairment. However, the mechanisms underlying improved near visual acuity are unknown. The present study compares feature search and oculomotor measures in 4-9 year old children with visual impairment accompanied by nystagmus (VI+nys [n=33] and children with normal vision (NV [n=29]. Children in the VI+nys group were divided into three training groups: an experimental PL group, a control PL group, and a magnifier group. They were seen before (baseline and after six weeks of training. Children with NV were only seen at baseline. The feature search task entailed finding a target E among distractor E’s (pointing right with element spacing varied in four steps: 0.04°, 0.5°, 1°, and 2°. At baseline, children with VI+nys showed longer search times, shorter fixation durations, and larger saccade amplitudes than children with NV. After training, all training groups showed shorter search times. Only the experimental PL group showed prolonged fixation duration after training at 0.5° and 2° spacing, p’s respectively .033 and .021. Prolonged fixation duration was associated with reduced crowding and improved crowded near visual acuity. One of the mechanisms underlying improved crowded near visual acuity after PL in children with VI+nys seems to be prolonged fixation duration.

  6. Three-cluster model for the α-accompanied fission of californium nuclei

    Science.gov (United States)

    Manimaran, K.; Balasubramaniam, M.

    2009-02-01

    A three-cluster model is proposed to explain the particle-accompanied binary fission of radioactive nuclei. The model is developed as an extension of the preformed cluster model of Gupta and collaborators. The advantage of this model is that, for a fixed third fragment, we can calculate the fragmentation potential minimized in charge coordinate. For our study we chose the various neutron-deficient to neutron-rich californium nuclei, whose analysis reveals that the closed-shell effect of any one of the fragments in ternary fragmentation presents itself as the most favorable configuration to be observed. As one goes from a neutron-deficient to a neutron-rich californium isotope, the role of the neutron closed shell associated with any one of the preferred fragments changes to that of the proton closed shell, and for very neutron rich isotopes of californium the presence of a double closed shell nucleus enhances the decay probability. The quadrupole deformation of the light fragment (A2) associated with the preferred configuration in the symmetric mass region also has a transition from positive to negative deformation as one goes from neutron-deficient to neutron-rich californium isotopes. The calculated relative yields of different fragmentation channels are compared with the available experimental yields for Cf252.

  7. Angular momenta of fission fragments in the α-accompanied fission of 252Cf

    Science.gov (United States)

    Jandel, M.; Kliman, J.; Krupa, L.; Morháč, M.; Hamilton, J. H.; Kormicki, J.; Ramayya, A. V.; Hwang, J. K.; Luo, Y. X.; Fong, D.; Gore, P.; Ter-Akopian, G. M.; Oganessian, Yu. Ts.; Rodin, A. M.; Fomichev, A. S.; Popeko, G. S.; Daniel, A. V.; Rasmussen, J. O.; Macchiavelli, A. O.; Stoyer, M. A.; Donangelo, R.; Cole, J. D.

    2005-06-01

    For the first time, average angular momenta of the ternary fission fragments 100, 102Zr, 106Mo, 144, 146Ba and 138, 140, 142Xe from the α-accompanied fission of 252Cf were obtained from relative intensities of prompt γ-ray transitions with the use of the statistical model calculation. Average values of the angular momenta were compared with the corresponding values for the same fission fragments from the binary fission of 252Cf. Results indicate the presence of a decreasing trend in the average values of angular momenta induced in ternary fission fragments compared to the same binary fission fragments. On the average, the total angular momentum extracted for ternary fission fragments is ˜1.4 ℏ lower than in binary fission. Consequently, results indicate that the mechanism of the ternary α-particles emission may directly effect an induction of angular momenta of fission fragments, and possible scenarios of such mechanisms are discussed. Further, the dependence of the angular momenta of 106Mo and 140Xe on the number of emitted neutrons from correlated pairs of primary fragments was obtained also showing a decreasing dependence of average angular momenta with increasing number of emitted neutrons. Consequences are briefly discussed.

  8. Long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome

    KAUST Repository

    Röthig, Till

    2016-02-03

    Scleractinian corals are assumed to be stenohaline osmoconformers, although they are frequently subjected to variations in seawater salinity due to precipitation, freshwater runoff, and other processes. Observed responses to altered salinity levels include differences in photosynthetic performance, respiration, and increased bleaching and mortality of the coral host and its algal symbiont, but a study looking at bacterial community changes is lacking. Here we exposed the coral Fungia granulosa to strongly increased salinity levels in short- and long-term experiments to disentangle temporal and compartment effects of the coral holobiont (i.e. coral host, symbiotic algae, and associated bacteria). Our results show a significant reduction in calcification and photosynthesis, but a stable microbiome after short-term exposure to high salinity levels. By comparison, long-term exposure yielded unchanged photosynthesis levels and visually healthy coral colonies indicating long-term acclimation to high salinity levels that were accompanied by a major coral microbiome restructuring. Importantly, a bacterium in the family Rhodobacteraceae was succeeded by Pseudomonas veronii as the numerically most abundant taxon. Further, taxonomy-based functional profiling indicates a shift in the bacterial community towards increased osmolyte production, sulfur oxidation, and nitrogen fixation. Our study highlights that bacterial community composition in corals can change within days to weeks under altered environmental conditions, where shifts in the microbiome may enable adjustment of the coral to a more advantageous holobiont composition.

  9. Pseudoaneurysm Accompanied by Crowe Type IV Developmental Dysplasia of the Hip: A Case Report

    Directory of Open Access Journals (Sweden)

    Hirotake Yo

    2012-01-01

    Full Text Available We report the case of a 72-year-old woman whose pseudoaneurysm was difficult to diagnose and treat. The patient had a history of congenital dislocated hip and was undergoing anticoagulation therapy with warfarin due to the mitral valve replacement. Her chief complaint was pain and enlargement of the left buttock, and the laboratory tests revealed severe anemia. However, her elderly depression confused her chief complaint, and she was transferred to a psychiatric hospital. Two months after the onset of the symptoms, she was finally diagnosed with a pseudoaneurysm by contrast-enhanced CT and angiography. IDC coils were used for embolization. A plain CT showed hemostasis as well as a reduced hematoma at 2 months after the embolization. The possible contributing factors for the pseudoaneurysm included bleeding due to warfarin combined with an intramuscular hematoma accompanied by Crowe type IV developmental dysplasia of the hip that led to an arterial rupture by impingement between pelvis and femoral head. Since the warfarin treatment could not be halted due to the valve replacement, embolization was chosen for her treatment, and the treatment outcome was favorable.

  10. Intracranial Hypertension in a Patient with a Chiari Malformation Accompanied by Hyperthyroidism.

    Science.gov (United States)

    Pang, Chang Hwan; Lee, Soo Eon; Kim, Chi Heon; Chung, Chun Kee

    2015-09-01

    The Chiari malformation is an infrequently detected congenital anomaly characterized by the downward displacement of the cerebellum with a tonsillar herniation below the foramen magnum that may be accompanied by either syringomyelia or hydrocephalus. Surgery, such as foramen magnum decompression, is indicated for a symptomatic Chiari malformation, although an incidental lesion may be followed-up without further treatment. Infrequently, increased intracranial pressure emerges due to hyperthyroidism. A nineteen-year-old girl visited our outpatient clinic presented with a headache, nausea and vomiting. A brain and spinal magnetic resonance image study (MRI) indicated that the patient had a Chiari I malformation without syringomyelia or hydrocephalus. An enlarged thyroid gland was detected on a physical examination, and serum markers indicated Graves' disease. The patient started anti-hyperthyroid medical treatment. Subsequently, the headache disappeared after the medical treatment of hyperthyroidism without surgical intervention for the Chiari malformation. A symptomatic Chiari malformation is indicated for surgery, but a surgeon should investigate other potential causes of the symptoms of the Chiari malformation to avoid unnecessary surgery.

  11. Associations between ulnar nerve strain and accompanying conditions in patients with cubital tunnel syndrome.

    Science.gov (United States)

    Ochi, Kensuke; Horiuchi, Yukio; Nakamura, Toshiyasu; Sato, Kazuki; Morita, Kozo; Horiuchi, Koichi

    2014-01-01

    Pathophysiology of cubital tunnel syndrome (CubTS) is still controversial. Ulnar nerve strain at the elbow was measured intraoperatively in 13 patients with CubTS before simple decompression. The patients were divided into three groups according to their accompanying conditions: compression/adhesion, idiopathic, and relaxation groups. The mean ulnar nerve strain was 43.5 ± 30.0%, 25.5 ± 14.8%, and 9.0 ± 5.0% in the compression/adhesion, idiopathic, and relaxation groups respectively. The mean ulnar nerve strains in patients with McGowan's classification grades I, II, and III were 18.0 ± 4.2%, 27.1 ± 22.7%, and 33.7 ± 24.7%, respectively. The Jonckheere-Terpstra test showed that there were significant reductions in the ulnar nerve strain among the first three groups, but not in the three groups according to McGowan's classification. Our results suggest that the pathophysiology, not disease severity, of CubTS may be explained at least in part by the presence of ulnar nerve strain.

  12. Risk Factors Accompanied with Nephropathy in Patients with Type II Diabetes; Test of the Biopsychosocial Model

    Directory of Open Access Journals (Sweden)

    I. Rahimian Boogar

    2012-07-01

    Full Text Available Introduction & Objective: The study of biopsychosocial factors influencing nephropathy as a most serious complication of type II diabetes is important. This study aimed to investigate risk factors accompanied with nephropathy in patients with type II diabetes based on the biopsychosocial model. Materials & Methods: In a cross-sectional descriptive study, 295 patients with type II diabetes were selected by convenience sampling in Tehran Shariati hospital outpatient clinics. The data were collected by demographical information questionnaire along with disease characteristics and depression anxiety stress scales (dass, quality of life scale (who- qol- bref, diabetes self-management scale (dsms, and diabetes knowledge scale (dks, then analyzed by chi-square, independent t-test and logistic regression with pasw software. Results: Hypertension (OR=3.841 & P0.05.Conclusion: It is important to pay attention to hypertension, glycated hemoglobin, body mass index, diabetes self-management, depression, quality of life, and diabetes knowledge for therapeutic intervention programming and diabetes complications control protocols for diabetic patients.(Sci J Hamadan Univ Med Sci 2012;19(2:44-53

  13. Policies of access to healthcare services for accompanied asylum-seeking children in the Nordic countries.

    Science.gov (United States)

    Sandahl, Hinuga; Norredam, Marie; Hjern, Anders; Asher, Henry; Nielsen, Signe Smith

    2013-08-01

    Asylum-seeking children constitute a vulnerable group with high prevalence and risk for mental health problems. The aim of this study was to compare policies of access to healthcare services, including physical examination and screening for mental health problems on arrival, for accompanied asylum-seeking children in the Nordic countries. This study was based on the national reports "Reception of refugee children in the Nordic countries" written by independent national experts for the Nordic Network for Research on Refugee Children, supplemented by information from relevant authorities. In Sweden, Norway and Iceland, asylum-seeking children had access to healthcare services equal to children in the general population. On a policy level, Denmark imposed restrictions on non-acute hospitalisations and prolonged specialist treatments. Regarding health examinations, Sweden deviated from the Nordic pattern by not performing these systematically. In Denmark, Iceland, and some counties in Sweden, but not in Norway, screening for mental health problems was offered to asylum-seeking children. Access to healthcare services for asylum-seeking children differs in the Nordic countries; the consequences of these systematic differences for the individual asylum-seeking child are unknown. For asylum-seeking children, access to healthcare has to be considered in a wider context that includes the core conditions of being an asylum-seeker. A comparative study at policy level needs to be supplemented with empirical follow-up studies of the well-being of the study population to document potential consequences of policies in practice.

  14. Similarity dependency of the change in ERP component N1 accompanying with the object recognition learning.

    Science.gov (United States)

    Tokudome, Wataru; Wang, Gang

    2012-01-01

    Performance during object recognition across views is largely dependent on inter-object similarity. The present study was designed to investigate the similarity dependency of object recognition learning on the changes in ERP component N1. Human subjects were asked to train themselves to recognize novel objects with different inter-object similarity by performing object recognition tasks. During the tasks, images of an object had to be discriminated from the images of other objects irrespective of the viewpoint. When objects had a high inter-object similarity, the ERP component, N1 exhibited a significant increase in both the amplitude and the latency variation across objects during the object recognition learning process, and the N1 amplitude and latency variation across the views of the same objects decreased significantly. In contrast, no significant changes were found during the learning process when using objects with low inter-object similarity. The present findings demonstrate that the changes in the variation of N1 that accompany the object recognition learning process are dependent upon the inter-object similarity and imply that there is a difference in the neuronal representation for object recognition when using objects with high and low inter-object similarity.

  15. Disturbance in the lower ionosphere that accompanied the reentry of the Chelyabinsk cosmic body

    Science.gov (United States)

    Chernogor, L. F.

    2017-09-01

    The paper describes quasi-periodic and aperiodic variations in the phase and amplitude of radio waves of LF and VLF ranges, which accompanied the flight and explosion of the Chelyabinsk meteoroid. Quasi-periodic variations in the phase have been explained by the generation of acoustic-gravity waves in the atmosphere, which modulate the electron density in the ionosphere and the phase of radio waves. Aperiodic variations in the phase and amplitude of radio waves are associated with an increase in the electron density in the lower ionosphere (at altitudes of 65-70 km). This increase was most likely caused by the interactions of subsystems in the Earth-atmosphere-ionosphere-magnetosphere system or, more correctly, by the precipitation of high-energy electrons from the magnetosphere into the lower ionosphere, which was stimulated by the flight and explosion of a cosmic body. According to the estimates, the density of the flux of electrons with energies of 100 KeV should be on the order of 106 m-2 s-1.

  16. Rapid magmatic processes accompany arc-continent collision: the Western Bismarck arc, Papua New Guinea

    Science.gov (United States)

    Cunningham, Heather; Gill, Jim; Turner, Simon; Caulfield, John; Edwards, Louise; Day, Simon

    2012-11-01

    New U-Th-Ra, major and trace element, and Sr-Nd-Pb isotope data are presented for young lavas from the New Britain and Western Bismarck arcs in Papua New Guinea. New Britain is an oceanic arc, whereas the latter is the site of an arc-continent collision. Building on a recent study of the Manus Basin, contrasts between the two arcs are used to evaluate the processes and timescales of magma generation accompanying arc-continent collision and possible slab detachment. All three suites share many attributes characteristic of arc lavas that can be ascribed to the addition of a regionally uniform subduction component derived from the subducting altered oceanic crust and sediment followed by dynamic melting of the modified mantle. However, the Western Bismarck arc lavas diverge from the Pb isotope mixing array formed by the New Britain and the Manus Basin lavas toward elevated 208Pb/204Pb. We interpret this to reflect a second and subsequent addition of sediment melt at crustal depth during collision. 238U and 226Ra excesses are preserved in all of the lavas and are greatest in the Western Bismarck arc. High-Mg andesites with high Sr/Y ratios in the westernmost arc are attributed to recent shallow mantle flux melting at the slab edge. Data for two historical rhyolites are also presented. Although these rhyolites formed in quite different tectonic settings and display different geochemical and isotopic compositions, both formed from mafic parents within millennia.

  17. Periodic fever accompanied by aphthous stomatitis, pharyngitis and cervical adenitis syndrome (PFAPA syndrome) in adults.

    Science.gov (United States)

    Padeh, Shai; Stoffman, Nava; Berkun, Yackov

    2008-05-01

    The new syndrome, known as PFAPA, of periodic fever characterized by abrupt onset of fever, malaise, aphthous stomatitis, tonsillitis, pharyngitis and cervical adenopathy has been described only in pediatric patients. It usually begins before the age of 5 years and in most cases resolves spontaneously before age 10. To describe a series of adults with PFAPA syndrome. This 6 year retrospective descriptive study includes all newly diagnosed incident adult cases aged 18 years and over referred to our center with symptomatology suggestive of PFAPA syndrome. Patients' medical records were reviewed for past history of the disease, demographic characteristics, symptoms and signs, course of the disease, laboratory findings, and outcome following corticosteroid therapy. The comparison group included our pediatric cohort children (N=320, age 0-18 years) followed for the last 14 years (1994-2008). Fifteen adult patients were diagnosed with PFAPA syndrome. Episodes of fever occurred at 4.6 +/- 1.3 week intervals, beginning at the age of 20.9 +/- 7.5. All patients had monthly attacks at the peak of the disease, with attacks recurring at 4-8 week intervals over the years. Between episodes the patients were apparently healthy, without any accompanying diseases. Attacks were aborted by a single 60 mg dose of oral prednisone in all patients. This study reports the presence of PFAPA syndrome in adult patients. Although the disease is rare, an increased awareness by both patients and family physicians of this clinical syndrome has resulted in more frequent diagnosis in adult patients.

  18. LUCC and Accompanied Soil Degradation in China from 1960's to 1990's

    Institute of Scientific and Technical Information of China (English)

    JIANG Qigang; Shintaro GOTO; Hiroki TAKAMURA

    2002-01-01

    This paper tries to qualitatively analyze land use and cover changes (LUCC) and accompanied soil degradation in China, the data of World Atlas of Agriculture in 1969, Land Cover Data of Asia in 1992 and Global Assessment of Human Induced Soil Degradation in 1992, etc. have been used. From 1960' s to 1990's, the area of forestland had increased, arable land and paddy as well as grassland had significantly decreased in China. The major type of soil degradation is due to water erosion, which is widely distributed especially in Loess Plateau and in Southeast and Southwest China, this happened in forestland, grassland and arable land and mainly resulted from deforestation. The secondary type is wind erosion, which is mainly distributed in Northwest China, it happened in arable land and grassland and was caused by overgrazing. Chemical deterioration is distributed in North and Northwest China, which happened in paddy, arable land and grassland. Physical deterioration only concentrated in local area in North China, it happened in arable and paddy. Chemical and physical deteriora-tion mainly resulted from unreasonable activity. Because of different human activities, different LUCC and different natural conditions, the types and strength of soil degradation can be different.

  19. Spin-flop transition accompanied with changing the type of magnetic ordering

    Science.gov (United States)

    Syromyatnikov, A. V.

    2017-03-01

    We discuss theoretically rather rear example of spin-flop transition which is accompanied with changing the type of magnetic ordering and which seemingly has not been addressed yet. We demonstrate that changing the type of magnetic ordering can manifest itself in antiferromagnetic (AF) resonance experiments as an apparent peculiar switching of the anisotropy at the transition from the easy-axis type to the easy-plane one. We argue that this kind of spin-flop transition is observed recently by Povarov et al. (2013) [12] in Cu(pz)2(ClO4)2 (where pz denotes pyrazine), one of the best realizations of spin-1/2 Heisenberg AFs on square lattice having a very small anisotropy. We show that the magnetic ordering changes at the spin-flop transition in this material in the direction perpendicular to AF square planes. We examine the microscopic mechanism of such behavior in Cu(pz)2(ClO4)2 and find that dipolar forces and extremely small exchange coupling between spins from neighboring planes are responsible for it.

  20. "El Sexo no es Malo": Maternal Values Accompanying Contraceptive Use Advice to Young Latina Adolescent Daughters.

    Science.gov (United States)

    Romo, Laura F; Bravo, Magali; Cruz, Maria Elena; Rios, Rebeca M; Kouyoumdjian, Claudia

    2010-06-01

    In this study, we utilized observational methods to identify maternal values and concerns accompanying contraceptive use advice in Latina mother-daughter sexuality conversations. The sample included non-sexually active early adolescents around 12 years of age and their mostly Spanish-speaking Latina mothers. Videotaped conversations were coded for the prevalence of messages related to four sexual values (abstinence, delay sex until older, sex is "normal", sex is "improper") and concerns about pregnancy and STD transmission. We examined whether the duration of time spent conversing about these messages was associated with participant characteristics, general communication openness, and the amount of time the dyads spent discussing contraceptive use. Results indicated that Latina mothers who had fewer years of education and lower family income talked longer to their daughters about the need to delay sex, avoid risky situations that would increase their chances of getting pregnant or acquiring an STD, and engage in self-protective practices. Less perceived openness in general communication as reported by both the mothers and the daughters was associated with increased time discussing that sex is improper. Although the duration of contraceptive use messages was brief, mothers and daughters who discussed the fact that sex is normal, and who communicated more about the importance of delaying sex, talked longer about contraceptive use practices compared to mothers and daughters who engaged in minimal discussion of these sexual values.

  1. Rapid increases in ventilation accompany the transition from passive to active movement.

    Science.gov (United States)

    Bell, Harold J; Duffin, James

    2006-06-01

    We used a novel movement transition technique to look for evidence of a rapid onset drive to breathe related to the active component of exercise in humans. Ten volunteers performed the following transitions in a specially designed tandem exercise chair apparatus: rest to passive movement, passive to active movement, and rest to active movement. The transition from rest to active exercise was accompanied by an immediate increase in ventilation, as was the transition from rest to passive leg movement (Delta = 6.06 +/- 1.09 l min(-1), p ventilation again increased immediately and significantly (Delta = 2.55 +/- 0.52 l min(-1), p = 0.032). Ventilation at the first point of active exercise was the same when started either from rest or from a background of passive leg movement (p = 1.00). We conclude that the use of a transition from passive to active leg movements in humans recruits a ventilatory drive related to the active component of exercise, and this can be discerned as a rapid increase in breathing.

  2. Anomaly in neural phase coherence accompanies reduced sensorimotor integration in adults who stutter.

    Science.gov (United States)

    Sengupta, Ranit; Shah, Shalin; Gore, Katie; Loucks, Torrey; Nasir, Sazzad M

    2016-12-01

    Despite advances in our understanding of the human speech system, the neurophysiological basis of stuttering remains largely unknown. Here, it is hypothesized that the speech of adults who stutter (AWS) is susceptible to disruptions in sensorimotor integration caused by neural miscommunication within the speech motor system. Human speech unfolds over rapid timescales and relies on a distributed system of brain regions working in a parallel and synchronized manner, and a breakdown in neural communication between the putative brain regions could increase susceptibility to dysfluency. Using a speech motor adaptation paradigm under altered auditory feedback with simultaneous recording of EEG, the oscillatory cortical dynamics was investigated in stuttering and fluent adults (FA). Auditory feedback perturbation involved the shifting of the formant frequencies of the target vowel sound. Reduced adaptation in response to the feedback error was observed in AWS and was accompanied by differences in EEG spectral powers and anomalies in phase coherence evolving over the course of speech motor training. It is understood that phase coherence possibly captures neural communication within speech motor networks. Thus, the phase coherence network of the two groups exhibited differences involving the EEG frequency bands. These findings in anomalous neural synchrony provide novel evidence for compromised neuronal communication at short time scales within the speech motor network of AWS.

  3. Solvent fluctuations around solvophobic, solvophilic, and patchy nanostructures and the accompanying solvent mediated interactions

    Science.gov (United States)

    Chacko, Blesson; Evans, Robert; Archer, Andrew J.

    2017-03-01

    Using classical density functional theory (DFT), we calculate the density profile ρ (𝐫 ) and local compressibility χ (𝐫 ) of a simple liquid solvent in which a pair of blocks with (microscopic) rectangular cross section are immersed. We consider blocks that are solvophobic, solvophilic and also ones that have both solvophobic and solvophilic patches. Large values of χ (𝐫 ) correspond to regions in space where the liquid density is fluctuating most strongly. We seek to elucidate how enhanced density fluctuations correlate with the solvent mediated force between the blocks, as the distance between the blocks and the chemical potential of the liquid reservoir vary. For sufficiently solvophobic blocks, at small block separations and small deviations from bulk gas-liquid coexistence, we observe a strongly attractive (near constant) force, stemming from capillary evaporation to form a low density gas-like intrusion between the blocks. The accompanying χ (𝐫 ) exhibits a structure which reflects the incipient gas-liquid interfaces that develop. We argue that our model system provides a means to understanding the basic physics of solvent mediated interactions between nanostructures, and between objects such as proteins in water that possess hydrophobic and hydrophilic patches.

  4. Chaos in oscillatory heat evolution accompanying the sorption of hydrogen and deuterium in palladium

    CERN Document Server

    Lalik, Erwin

    2012-01-01

    Aperiodic oscillations in the sorption of H2 or D2 in metallic Pd powder have been observed, and a novel method to confirm their deterministic rather than random character has been devised. A theorem relating the square of a function, with the derivative and integral with variable upper limit of the same function has been proved and proposed to be used as a base for a chaos-vs-random test. Both the experimental and the computed time series may be tested to detect determinism. The result is a single number within the interval [0,2]. The test is designed in such a way that its result is close to zero for the datasets that are deterministic and smooth, and close to 2 for the datasets that are non deterministic (random) or non smooth (discrete). A large variety of the test results has been obtained for the calorimetric time series recorded in thermokinetic oscillations, periodic and quasiperiodic, accompanying the sorption of H2 or D2 with Pd as well as for several non oscillatory calorimetric curves recorded in ...

  5. Morphological brain plasticity induced by musical expertise is accompanied by modulation of functional connectivity at rest.

    Science.gov (United States)

    Fauvel, Baptiste; Groussard, Mathilde; Chételat, Gaël; Fouquet, Marine; Landeau, Brigitte; Eustache, Francis; Desgranges, Béatrice; Platel, Hervé

    2014-04-15

    The aim of this study was to explore whether musical practice-related gray matter increases in brain regions are accompanied by modifications in their resting-state functional connectivity. 16 young musically experienced adults and 17 matched nonmusicians underwent an anatomical magnetic resonance imaging (MRI) and a resting-state functional MRI (rsfMRI). A whole-brain two-sample t test run on the T1-weighted structural images revealed four clusters exhibiting significant increases in gray matter (GM) volume in the musician group, located within the right posterior and middle cingulate gyrus, left superior temporal gyrus and right inferior orbitofrontal gyrus. Each cluster was used as a seed region to generate and compare whole-brain resting-state functional connectivity maps. The two clusters within the cingulate gyrus exhibited greater connectivity for musicians with the right prefrontal cortex and left temporal pole, which play a role in autobiographical and semantic memory, respectively. The cluster in the left superior temporal gyrus displayed enhanced connectivity with several language-related areas (e.g., left premotor cortex, bilateral supramarginal gyri). Finally, the cluster in the right inferior frontal gyrus displayed more synchronous activity at rest with claustrum, areas thought to play a role in binding sensory and motor information. We interpreted these findings as the consequence of repeated collaborative use in general networks supporting some of the memory, perceptual-motor and emotional features of musical practice. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Mass changes accompanying the pseudocapacitance of hydrous RuO{sub 2} under different experimental conditions

    Energy Technology Data Exchange (ETDEWEB)

    Sopcic, Suzana; Rokovic, Marijana Kraljic [Faculty of Chemical Engineering and Technology, Department of Electrochemistry, University of Zagreb, Marulicev trg 19, HR-10000 Zagreb (Croatia); Mandic, Zoran, E-mail: zmandic@fkit.h [Faculty of Chemical Engineering and Technology, Department of Electrochemistry, University of Zagreb, Marulicev trg 19, HR-10000 Zagreb (Croatia); Roka, Andras [Department of Physical Chemistry, Eoetvoes Lorand University, Pazmany Peter setany 1/A, H-1117 Budapest (Hungary); Inzelt, Gyoergy, E-mail: inzeltgy@chem.elte.h [Department of Physical Chemistry, Eoetvoes Lorand University, Pazmany Peter setany 1/A, H-1117 Budapest (Hungary)

    2011-04-01

    Pseudocapacitance reaction of hydrous ruthenium oxide was investigated by cyclic voltammetry combined with electrochemical quartz-crystal nanobalance (EQCN) in sulfuric acid as well as in neutral solutions of Na{sub 2}SO{sub 4} and K{sub 2}SO{sub 4}. The ruthenium oxide electrode was prepared by attaching the ruthenium oxide particles on gold covered quartz electrode. The results show that there are different types of charge taking place simultaneously during the redox reaction of ruthenium oxide electrode. Their contribution to the overall charge depends on the experimental conditions. Depending on the potential and electrolyte used the redox reaction of ruthenium oxide is accompanied either by mass loss or by mass gain. The average molar masses of the species exchanged between the solid phase and the electrolyte solution depend on the potential and scan rate. The effect of Nafion{sup TM} top layer was also investigated. It has been found that it does not affect significantly the overall specific capacitance of ruthenium oxide electrode but the apparent molar masses of exchanged species decrease in comparison with the uncovered electrodes.

  7. A fatal case of severe serotonin syndrome accompanied by moclobemide and paroxetine overdose

    Directory of Open Access Journals (Sweden)

    Sener Serkan

    2005-01-01

    Full Text Available Aim: To present a fatal case of serotonin syndrome accompanied by moclobemide and paroxetine overdose. Case presentation: A 34-year-old married woman was presented following intentional ingestion of 3.5 g moclobemide and 2.6 g paroxetine. She was drowsy, agitated, and having rigor. In 1 h she developed myoclonus and diffuse muscle rigidity prominent in lower extremities. All laboratory tests were unremarkable except hyperglycemia (160 mg/dl, sinus tachycardia (103/min, and metabolic acidosis (7.051 pH, 52 mmHg pO2, 74.7 mmHg pCO2, 15% HCO3, 77% SaO2. Despite oxygen supplementation, her respiratory acidosis got worse and the SaO2 concentration decreased to 72%. Endotracheal intubation and paralysis were decided to control muscle hyperactivity followed by hyperthermia (max. 42.3°C unresponsive to benzodiazepine. Even aggressive supportive treatment (mechanical ventilation, buffer replacement, cyproheptadine, and dantrolene were applied, the patient could not recover and suffered cardiopulmonary arrest 20 h after presentation. Conclusion: Physicians working in the emergency departments and intensive care units, managing patients presenting with acute ingestion of selective serotonin reuptake inhibitors combined with monoamine oxidase inhibitors, should be aware of recognizing and treating serotonin stndrome. This is because many of these patients may require intensive care monitoring as well as tracheal intubation and ventilatory support.

  8. [A synovial cyst accompanied by asymptomatic lumbar vertebral fracture requiring differentiation from spinal metastasis].

    Science.gov (United States)

    Miura, Isamu; Ujiie, Hiroshi; Nakagawa, Masanori; Saito, Taiichi; Shiono, Saori; Okada, Yoshikazu

    2015-06-01

    We experienced a case with a synovial cyst accompanied by asymptomatic lumbar vertebral fracture that required differentiation from spinal metastasis. An 82-year-old man suffered from right leg and anal pain. Computed tomography (CT) showed L5 spondylolysis. Magnetic resonance images (MRI) revealed an intra spinal cyst and acute lumbar vertebral fracture of L5 vertebral body. The surrounding area of the cyst presented contrast enhancement, and the extradural mass compressed the dural sac. Bone scintigraphy with 99m technetium-MDP demonstrated intense uptake on the right first, fourth, fifth, and seventh ribs and L2, L3, and L5 vertebra. The F-18 fluorodeoxyglucose positron emission tomography (FDG-PET) image demonstrated an increased radiotracer uptake in the L5 vertebra(standardized uptake value(SUV) max=3.5). Spinal metastasis was suspected. Because of the cauda equina compression syndrome, it was surgically removed. Intraoperatively, a well-demarcated extradural cyst was found and compressed the dural sac markedly. The cyst capsule was thin and contained clear, thin fluid with no signs of bleeding. The histological diagnosis was a synovial cyst. His neurological symptoms improved after the surgery. The synovial cyst may enlarge after asymptomatic vertebral fractures.

  9. Long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome.

    Science.gov (United States)

    Röthig, Till; Ochsenkühn, Michael A; Roik, Anna; van der Merwe, Riaan; Voolstra, Christian R

    2016-03-01

    Scleractinian corals are assumed to be stenohaline osmoconformers, although they are frequently subjected to variations in seawater salinity due to precipitation, freshwater run-off and other processes. Observed responses to altered salinity levels include differences in photosynthetic performance, respiration and increased bleaching and mortality of the coral host and its algal symbiont, but a study looking at bacterial community changes is lacking. Here, we exposed the coral Fungia granulosa to strongly increased salinity levels in short- and long-term experiments to disentangle temporal and compartment effects of the coral holobiont (i.e. coral host, symbiotic algae and associated bacteria). Our results show a significant reduction in calcification and photosynthesis, but a stable microbiome after short-term exposure to high-salinity levels. By comparison, long-term exposure yielded unchanged photosynthesis levels and visually healthy coral colonies indicating long-term acclimation to high-salinity levels that were accompanied by a major coral microbiome restructuring. Importantly, a bacterium in the family Rhodobacteraceae was succeeded by Pseudomonas veronii as the numerically most abundant taxon. Further, taxonomy-based functional profiling indicates a shift in the bacterial community towards increased osmolyte production, sulphur oxidation and nitrogen fixation. Our study highlights that bacterial community composition in corals can change within days to weeks under altered environmental conditions, where shifts in the microbiome may enable adjustment of the coral to a more advantageous holobiont composition.

  10. Late time observations of GRB080319B: jet break, host galaxy and accompanying supernova

    CERN Document Server

    Tanvir, Nial R; Levan, Andrew; Fruchter, Andrew; Granot, Jonathan; Svensson, Karl M; O'Brien, Paul T; Wiersema, Klaas; Starling, Rhaana L C; Jakobsson, Pall; Fynbo, Johan; Hjorth, Jens; Curran, Peter; van der Horst, Alexander J; Kouveliotou, Chryssa; Racusin, Judith L; Burrows, David N; Genet, Frank

    2008-01-01

    The Swift-discovered GRB080319B was by far the most distant source ever observed at naked eye brightness, reaching a peak magnitude of 5.3 at a redshift of z=0.937. We present our late time optical and X-ray observations, which confirm that an achromatic break occurred in the power-law afterglow light curve at ~10^6 s post-burst. This most likely indicates that the gamma-ray burst (GRB) outflow was collimated, which for a uniform jet would imply a total energy in the jet E_{jet} \\gsim 10^{52.5} erg. Our observations also show a late-time excess of red light, which is well explained if the GRB was accompanied by a supernova, similar to those seen in some other long-duration GRBs. The latest observations are dominated by light from the host and show that the GRB took place in a faint dwarf galaxy (r(AB) = 27.2, rest-frame M_B = -17.3). This galaxy is small even by the standards of other GRB hosts, which is suggestive of a low metallicity environment.

  11. Motor imagery modulation of postural sway is accompanied by changes in the EMG-COP association.

    Science.gov (United States)

    Lemos, Thiago; Rodrigues, Erika C; Vargas, Claudia D

    2014-08-08

    Motor imagery (MI) performed in an upright stance promotes increases in postural sway without changes in usual amplitude measures of calf muscle EMG. However, postural muscle activity can also be determined from the temporal association between EMG and center of pressure (COP) displacements. In this study we investigated whether the MI modulation of postural sway is accompanied by changes in EMG-COP association. Surface EMG from the lateral gastrocnemius (LG) muscle and COP coordinates were collected from 12 subjects while they imagined themselves performing a rising on tiptoes movement via kinesthetic or visual imagery. As a control condition subjects were requested to imagine singing a song. The standard deviation of the forward-backward COP sway and the coefficient of variation of the EMG were calculated and compared across tasks. The degree of association between COP sways and LG activity was evaluated through a cross-correlation function. Kinesthetic imagery promoted a larger COP displacement than both visual and control imagery (pEMG amplitude was observed across imagery tasks (p=0.08). Crucially, we found a stronger EMG-COP association during kinesthetic imagery compared to control imagery (p=0.02), whereas the EMG-COP association in visual imagery was not different from that observed during kinesthetic or control imagery (p>0.19). In conclusion, kinesthetic imagery resulted in a higher EMG-COP temporal association. Subliminal fringe mechanisms may account for the imagery effects on muscle activity and postural sway during upright stance.

  12. GRB off-axis afterglows and the emission from the accompanying supernovae

    CERN Document Server

    Kathirgamaraju, Adithan; Giannios, Dimitrios

    2016-01-01

    Gamma-Ray Burst (GRB) afterglows are likely produced in the shock that is driven as the GRB jet interacts with the external medium. Long duration GRBs are also associated with powerful supernovae (SN). We consider the optical and radio afterglows of long GRBs for both blasts viewed along the jet axis ("on-axis" afterglows) and misaligned observes ("off-axis" afterglows). Comparing the optical emission from the afterglow with that of the accompanying SN, using SN 1998bw as an archetype, we find that only a few percent of afterglows viewed off-axis are brighter than the SN. For observable optical off-axis afterglows the viewing angle is at most twice the half-opening angle of the GRB jet. Radio off-axis afterglows should be detected with upcoming radio surveys within a few hundred Mpc. We propose that these surveys will act as "radio triggers," and that dedicated radio facilities should follow-up these sources. Follow-ups can unveil the presence of the radio supernova remnant, if present. In addition, they can ...

  13. GRB off-axis afterglows and the emission from the accompanying supernovae

    Science.gov (United States)

    Kathirgamaraju, Adithan; Barniol Duran, Rodolfo; Giannios, Dimitrios

    2016-09-01

    Gamma-ray burst (GRB) afterglows are likely produced in the shock that is driven as the GRB jet interacts with the external medium. Long-duration GRBs are also associated with powerful supernovae (SNe). We consider the optical and radio afterglows of long GRBs for both blasts viewed along the jet axis (`on-axis' afterglows) and misaligned observes (`off-axis' afterglows). Comparing the optical emission from the afterglow with that of the accompanying SN, using SN 1998bw as an archetype, we find that only a few per cent of afterglows viewed off-axis are brighter than the SN. For observable optical off-axis afterglows, the viewing angle is at most twice the half-opening angle of the GRB jet. Radio off-axis afterglows should be detected with upcoming radio surveys within a few hundred Mpc. We propose that these surveys will act as `radio triggers', and that dedicated radio facilities should follow-up these sources. Follow-ups can unveil the presence of the radio SN remnant, if present. In addition, they can probe the presence of a mildly relativistic component, either associated with the GRB jet or the SN ejecta, expected in these sources.

  14. Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems.

    Science.gov (United States)

    Kunugi, Motoshi; Satoh, Soichirou; Ihara, Kunio; Shibata, Kensuke; Yamagishi, Yukimasa; Kogame, Kazuhiro; Obokata, Junichi; Takabayashi, Atsushi; Tanaka, Ayumi

    2016-06-01

    Photosynthetic organisms have various pigments enabling them to adapt to various light environments. Green plants are divided into two groups: streptophytes and chlorophytes. Streptophytes include some freshwater green algae and land plants, while chlorophytes comprise the other freshwater green algae and seawater green algae. The environmental conditions driving the divergence of green plants into these two groups and the changes in photosynthetic properties accompanying their evolution remain unknown. Here, we separated the core antennae of PSI and the peripheral antennae [light-harvesting complexes (LHCs)] in green plants by green-native gel electrophoresis and determined their pigment compositions. Freshwater green algae and land plants have high Chl a/b ratios, with most Chl b existing in LHCs. In contrast, seawater green algae have low Chl a/b ratios. In addition, Chl b exists not only in LHCs but also in PSI core antennae in these organisms, a situation beneficial for survival in deep seawater, where blue-green light is the dominant light source. Finally, low-energy Chl (red Chl) of PSI was detected in freshwater green algae and land plants, but not in seawater green algae. We thus conclude that the different level of Chl b accumulation in core antennae and differences in PSI red Chl between freshwater and seawater green algae are evolutionary adaptations of these algae to their habitats, especially to high- or low-light environments.

  15. Frontal mucocele with an accompanying orbital abscess mimicking a fronto-orbital mucocele: case report

    Directory of Open Access Journals (Sweden)

    Akkuzu Guzin

    2006-04-01

    Full Text Available Abstract Background Mucoceles are slowly expanding cystic lesions with respiratory epithelium containing mucus most commonly affecting the frontal and ethmoidal sinuses. They are caused by obstruction of sinus ostium. Mucoceles exert pressure on the bony boundaries and due to the proximity to the brain and orbit extension to these areas are common. Case presentation A case of a frontal mucocele with an accompanying orbital abscess mimicking a fronto-orbital mucocele is reported. A 77 year old female patient suffering from left sided proptosis and pain around the left eye was admitted to our department. She had a history of left frontal sinus mucocele one year ago that was offered an osteoplastic frontal sinus surgery that the patient refused. Patient had limitation of eye movements. Fundoscopic examination revealed a minimal papilledema. Coronal computerized tomography and orbital magnetic resonance imaging showed a frontal mucocele with suspicious erosion of the orbital roof and a superiorly localized extraconal mass displacing the orbit lateroinferiorly. Frontal and orbital masses had similar intensities. Thus surgery was planned for a fronto-orbital mucocele. During surgery no defect was found on the orbital roof. Frontal mucocele and orbital cystic mass was removed separately. Pathological examination showed a frontal mucocele and an orbital abscess wall. Postoperatively eye movements returned to normal and papilledema resolved. Conclusion Fronto-orbital mucoceles are commonly encountered pathologies, but frontal mucocele with an orbital abscess is a rarely seen and should be kept in mind because their treatments differ.

  16. Neuroinflammation and α-synuclein accumulation in response to glucocerebrosidase deficiency are accompanied by synaptic dysfunction.

    Science.gov (United States)

    Ginns, Edward I; Mak, Sally K-K; Ko, Novie; Karlgren, Juliane; Akbarian, Schahram; Chou, Vivian P; Guo, Yin; Lim, Arlene; Samuelsson, Steven; LaMarca, Mary L; Vazquez-DeRose, Jacqueline; Manning-Boğ, Amy B

    2014-02-01

    Clinical, epidemiological and experimental studies confirm a connection between the common degenerative movement disorder Parkinson's disease (PD) that affects over 1 million individuals, and Gaucher disease, the most prevalent lysosomal storage disorder. Recently, human imaging studies have implicated impaired striatal dopaminergic neurotransmission in early PD pathogenesis in the context of Gaucher disease mutations, but the underlying mechanisms have yet to be characterized. In this report we describe and characterize two novel long-lived transgenic mouse models of Gba deficiency, along with a subchronic conduritol-ß-epoxide (CBE) exposure paradigm. All three murine models revealed striking glial activation within nigrostriatal pathways, accompanied by abnormal α-synuclein accumulation. Importantly, the CBE-induced, pharmacological Gaucher mouse model replicated this change in dopamine neurotransmission, revealing a markedly reduced evoked striatal dopamine release (approximately 2-fold) that indicates synaptic dysfunction. Other changes in synaptic plasticity markers, including microRNA profile and a 24.9% reduction in post-synaptic density size, were concomitant with diminished evoked dopamine release following CBE exposure. These studies afford new insights into the mechanisms underlying the Parkinson's-Gaucher disease connection, and into the physiological impact of related abnormal α-synuclein accumulation and neuroinflammation on nigrostriatal dopaminergic neurotransmission. Copyright © 2013 Elsevier Inc. All rights reserved.

  17. Preference Reversals in Decision Making Under Risk are Accompanied by Changes in Attention to Different Attributes

    Science.gov (United States)

    Kim, Betty E.; Seligman, Darryl; Kable, Joseph W.

    2012-01-01

    Recent work has shown that visual fixations reflect and influence trial-to-trial variability in people’s preferences between goods. Here we extend this principle to attribute weights during decision making under risk. We measured eye movements while people chose between two risky gambles or bid on a single gamble. Consistent with previous work, we found that people exhibited systematic preference reversals between choices and bids. For two gambles matched in expected value, people systematically chose the higher probability option but provided a higher bid for the option that offered the greater amount to win. This effect was accompanied by a shift in fixations of the two attributes, with people fixating on probabilities more during choices and on amounts more during bids. Our results suggest that the construction of value during decision making under risk depends on task context partly because the task differentially directs attention at probabilities vs. amounts. Since recent work demonstrates that neural correlates of value vary with visual fixations, our results also suggest testable hypotheses regarding how task context modulates the neural computation of value to generate preference reversals. PMID:22833715

  18. Presentation of new tectonic map (and accompanying sections) of Trinidad and Tobago

    Energy Technology Data Exchange (ETDEWEB)

    Persad, K.M.

    1984-04-01

    The Geologic Map of Trinidad, compiled by H.G. Kugler and published in 1961, is currently out of print. While it is the most widely available geologic map, it is confined to onshore Trinidad. This map remains an important reference source, but there have been significant increases in our knowledge of Trindad and Tobago geology since its publication. In particular, there has been: (1) considerable geophysical work, on land and offshore, including 20,000 km (12,400 mi) of seismic lines; (2) approximately 1000 exploration and development wells drilled, including wells in the previously unexplored north and east coast of Trinidad; and (3) significant advances in our understanding of the tectonic evolution of the area, which has resulted largely from the development of the plate tectonic theory. The following items, which take into account many of these new data and concepts, have been compiled. (1) A geologic tectonic map of the entire territory of Trinidad and Tobago, at a scale of 1:200,000. Apart from the surface geology of the land areas, this map shows the major faults and their displacements and locations, total depths and status of exploration wells, and the positions of major petroleum fields. (2) Five accompanying geologic sections at the same scale. (3) A new stratigraphic correlation chart. These new compilations attempt to fill the gap in the published literature on the petroleum geology of Trinidad and Tobago.

  19. Enhanced superconductivity accompanying a Lifshitz transition in electron-doped FeSe monolayer

    Science.gov (United States)

    Shi, X.; Han, Z.-Q.; Peng, X.-L.; Richard, P.; Qian, T.; Wu, X.-X.; Qiu, M.-W.; Wang, S. C.; Hu, J. P.; Sun, Y.-J.; Ding, H.

    2017-04-01

    The origin of enhanced superconductivity over 50 K in the recently discovered FeSe monolayer films grown on SrTiO3 (STO), as compared to 8 K in bulk FeSe, is intensely debated. As with the ferrochalcogenides AxFe2-ySe2 and potassium-doped FeSe, which also have a relatively high-superconducting critical temperature (Tc), the Fermi surface (FS) of the FeSe/STO monolayer films is free of hole-like FS, suggesting that a Lifshitz transition by which these hole FSs vanish may help increasing Tc. However, the fundamental reasons explaining this increase of Tc remain unclear. Here we report a 15 K jump of Tc accompanying a second Lifshitz transition characterized by the emergence of an electron pocket at the Brillouin zone centre, which is triggered by high-electron doping following in situ deposition of potassium on FeSe/STO monolayer films. Our results suggest that the pairing interactions are orbital dependent in generating enhanced superconductivity in FeSe.

  20. Discrete molecular states in the brain accompany changing responses to a vocal signal

    Science.gov (United States)

    Dong, Shu; Replogle, Kirstin L.; Hasadsri, Linda; Imai, Brian S.; Yau, Peter M.; Rodriguez-Zas, Sandra; Southey, Bruce R.; Sweedler, Jonathan V.; Clayton, David F.

    2009-01-01

    New experiences can trigger changes in gene expression in the brain. To understand this phenomenon better, we studied zebra finches hearing playbacks of birdsong. Earlier research had shown that initial playbacks of a novel song transiently increase the ZENK (ZIF-268, EGR1, NGFIA, KROX-24) mRNA in the auditory forebrain, but the response selectively habituates after repetition of the stimulus. Here, using DNA microarray analysis, we show that novel song exposure induces rapid changes in thousands of RNAs, with even more RNAs decreasing than increasing. Habituation training leads to the emergence of a different gene expression profile a day later, accompanied by loss of essentially all of the rapid “novel” molecular responses. The novel molecular profile is characterized by increases in genes involved in transcription and RNA processing and decreases in ion channels and putative noncoding RNAs. The “habituated” profile is dominated by changes in genes for mitochondrial proteins. A parallel proteomic analysis [2-dimensional difference gel electrophoresis (2D-DIGE) and sequencing by mass spectrometry] also detected changes in mitochondrial proteins, and direct enzyme assay demonstrated changes in both complexes I and IV in the habituated state. Thus a natural experience, in this case hearing the sound of birdsong, can lead to major shifts in energetics and macromolecular metabolism in higher centers in the brain. PMID:19541599

  1. Why Gravitational Contraction Must be Accompanied by Emission of RAdiation Both in Newtonian and Einstein Gravity

    CERN Document Server

    Mitra, A

    2006-01-01

    Helmholtz and Kelvin sowed that the contraction of a bound self-gravitating system must be accompanied by release of radiation energy irrespective of the details of the contraction process because the total Newtonian energy of the system E_N decreases for such contraction. In the era of General Relativity (GR) too, it is justifiably believed that gravitational contraction must release radiation energy. However no GR version of Helmholtz-Kelvin (HK) process has ever been derived. On the other hand, the same Newtonian form of the HK process is used by replacing the relevant quantities by the GR proper values. Here, for the first time, we derive the GR version of this important astrophysical process by simply equating the well known expressions for the gravitational mass (Schwarzschild Mass) and the Inertial Mass (time component of linear momentum) of a spherically symmetric static fluid. Though the resultant equations are different from their presumed form (used so far), GR KH theorem indeed shows that gravitat...

  2. Demands, subjectivity and therapeutic process: construction and limitations of Therapeutic Accompaniment

    Directory of Open Access Journals (Sweden)

    Luís Felipe Ferro

    2014-12-01

    Full Text Available The Brazilian Psychiatric Reform movement proposed several changes in the field of mental health. Since then, guidelines have been redirecting actions to respond to different demands, associating social inclusion with clinical follow-up. In this context, Therapeutic Accompaniment (TA appears as an important device to compose interventions focused on the demands of individuals with mental disorders. Thus the present work aims to present reflections on the limitations and potentials of TA. This study was based on the practice of TA in the Occupational Therapy Traineeship in Mental Health of the Federal University of Parana - UFPR. Regarding the methodological framework, this paper is structured in a two-way approach: presentation of a case report and exploratory research using unstructured interviews focused on two subjects monitored by TA. Hermeneutic-dialectic analysis was used for data treatment. The results indicate that TA provides significant gains to the process of rehabilitation and construction of citizenship of individuals with mental disorders, enabling actions focused on the demands of the individual, family and community. Nevertheless, there is still the need for continuity of TA interventions, as well as some structural deadlocks that tend to decrease the power of this device.

  3. Application of near-infrared spectroscopy to measurement of hemodynamic signals accompanying stimulated saliva secretion.

    Science.gov (United States)

    Sato, Hiroki; Obata, Akiko N; Moda, Ichiro; Ozaki, Kazutaka; Yasuhara, Takaomi; Yamamoto, Yukari; Kiguchi, Masashi; Maki, Atsushi; Kubota, Kisou; Koizumi, Hideaki

    2011-04-01

    We aim to test the feasibility of using near-infrared spectroscopy (NIRS) for indirect measurement of human saliva secretion in response to taste stimuli for potential application to organoleptic testing. We use an NIRS system to measure extracranial hemodynamics (Hb-signals around the temples) of healthy participants when taste stimuli are taken in their mouths. First, the Hb-signals and volume of expelled saliva (stimulated by distilled-water or sucrose-solution intake) are simultaneously measured and large Hb-signal changes in response to the taste stimuli (Hb-responses) are found. Statistical analysis show that both the Hb response and saliva volume are larger for the sucrose solution than for the distilled water with a significant correlation between them (r = 0.81). The effects of swallowing on the Hb-signals are investigated. Similar Hb responses, differing from the sucrose solution and distilled water, are obtained even though the participants swallow the mouth contents. Finally, functional magnetic resonance imaging is used to identify possible sources of the Hb signals corresponding to salivation. Statistical analysis indicates similar responses in the extracranial regions, mainly around the middle meningeal artery. In conclusion, the identified correlation between extracranial hemodynamics and the saliva volume suggests that NIRS is applicable to the measurement of hemodynamic signals accompanying stimulated saliva secretion.

  4. Neuromagnetic fields accompanying unilateral and bilateral voluntary movements: topography and analysis of cortical sources.

    Science.gov (United States)

    Kristeva, R; Cheyne, D; Deecke, L

    1991-08-01

    Movement-related magnetic fields (MRMFs) accompanying left and right unilateral and bilateral finger flexions were studied in 6 right-handed subjects. Six different MRMF components occurring prior to, and during both unilateral and bilateral movements are described: a slow pre-movement readiness field (RF, 1-0.5 sec prior to movement onset); a motor field (MF) starting shortly before EMG onset; 3 separate "movement-evoked" fields following EMG onset (MEFI at 100 msec; MEFII at 225 msec; and MEFIII at 320 msec); and a "post-movement" field (PMF) following the movement itself. The bilateral topography of the RF and MF for both unilateral and bilateral movements suggested bilateral generators for both conditions. Least-squares fitting of equivalent current dipole sources also indicated bilateral sources for MF prior to both unilateral and bilateral movements with significantly greater strength of contralateral sources in the case of unilateral movements. Differences in pre-movement field patterns for left versus right unilateral movements indicated possible cerebral dominance effects as well. A single current dipole in the contralateral sensorimotor cortex could account for the MEFI for unilateral movements and bilateral sensorimotor sources for bilateral movements. Other MRMF components following EMG onset indicated similar sources in sensorimotor cortex related to sensory feedback or internal monitoring of the movement. The results are discussed with respect to the possible generators active in sensorimotor cortex during unilateral and bilateral movement preparation and execution and their significance for the study of cortical organization of voluntary movement.

  5. A Brief Analysis on Dance Piano Accompaniment Teaching%舞蹈钢琴伴奏教学浅析

    Institute of Scientific and Technical Information of China (English)

    冷平

    2011-01-01

    The essential point of the paper is trying to explore the relationship between dance and music,the position of piano accompaniment in dance performance,how dance moves and piano accompaniment adapt to music styles,and how music sense and artistic accomplishment play important role on promoting dance piano accompaniment and so on.Dance piano accompaniment is not subordinate to dance.On the contrary,piano accompaniment can strengthen the dance emotion and endow with movement and vitality in the classroom teaching activities.Besides,it can make students more easily understand the content of dance training and improve dance teaching quality.%舞蹈与音乐是相生相灭、相辅相成的.钢琴伴奏是当今舞蹈教学中不可缺少的环节,是舞蹈教学中的重要组成部分.充分发挥钢琴伴奏在舞蹈教学中的作用,达到音乐与舞蹈完美的融合,这对舞蹈钢琴伴奏者提出了较高的专业要求.

  6. Correction: Lactogenic differentiation of HC11 cells is not accompanied by downregulation of AP-2 transcription factor genes

    LENUS (Irish Health Repository)

    Jager, Richard

    2011-09-09

    AbstractFollowing the publication of our article, an error in Table 1 was noted. The primer sequences used for PCR of beta-casein are incorrectly stated. The beta-casein primer pair has been incorrectly stated as: beta-casein sense: 5\\'-CCATCCTGCGTCTGGACCTG-3\\' beta-casein antisense: 5\\'-GGAATGTTGTGGAGTGGCAG-3\\' The correct beta-casein primer pair that has been used in the study is: beta-casein sense: 5\\'-GCCTTGCCAGTCTTGCTAAT-3\\' beta-casein antisense: 5\\'-GGAATGTTGTGGAGTGGCAG-3\\' We apologise for any inconvenience this may have caused.

  7. A case of Hypereosinophilic Syndrome Accompanied with Lung Cancer%嗜酸细胞增多性皮炎伴肺癌1例

    Institute of Scientific and Technical Information of China (English)

    宋文婷; 赵广

    2012-01-01

    患者女,60岁,躯干、四肢皮肤干燥、脱屑伴瘙痒1年余,加重1月.近5月出现咳嗽、咳痰,加重伴胸闷、憋气一周.皮肤组织病理:表皮内水肿,棘层肥厚,皮突延长,真皮血管周围见大量嗜酸性粒细胞.胸部CT:肺恶性肿瘤.胸部肿物病理示:低分化腺癌,局灶伴鳞癌分化.诊断:嗜酸细胞增多性皮炎;肺癌.%Female patient, 60 years old, presented with a one-year history of skin dryness and peeling with pruritus on the trunk and four limbs, worsing for one month. In recent 5 months,the patient have been suffered from worsing cough and expectoration for one week accompanied with dyspnea. Pathological examination showed epidermis with dropsy, acanthosis and elongation of the rete ridge. Infiltration of dense eosinophils surrounding of vessels were seen in the dermis. Chest CT showed lung cancer. Histopathology showed poorly differen-tiated adenocareinoma with focal differentiation of squamous cell carcinoma. Diagnosis of eosinophilia dermatitis accompanied with lung cancer was made.

  8. Absorption of nitric oxide into aqueous solutions of ferrous chelates accompanied by instantaneous reaction

    NARCIS (Netherlands)

    Demmink, J.F; vanGils, I.C.F.; Beenackers, A.A C M

    1997-01-01

    The absorption of nitric oxide (NO) into aqueous solutions of ferrous chelates of nitrilotriacetic acid (NTA), ethylene diaminetetraacetic acid (EDTA), hydroxyethylenediaminetriacetic acid (HEDTA), and diethylenetriaminepentaacetic acid (DTPA) was studied in a stirred cell reactor. Experimental cond

  9. Absorption of nitric oxide into aqueous solutions of ferrous chelates accompanied by instantaneous reaction

    NARCIS (Netherlands)

    Demmink, J.F; vanGils, I.C.F.; Beenackers, A.A C M

    1997-01-01

    The absorption of nitric oxide (NO) into aqueous solutions of ferrous chelates of nitrilotriacetic acid (NTA), ethylene diaminetetraacetic acid (EDTA), hydroxyethylenediaminetriacetic acid (HEDTA), and diethylenetriaminepentaacetic acid (DTPA) was studied in a stirred cell reactor. Experimental

  10. Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord

    Directory of Open Access Journals (Sweden)

    Kucharova Karolina

    2011-11-01

    Full Text Available Abstract Background Multiple sclerosis (MS is a demyelinating disease in which blood-derived immune cells and activated microglia damage myelin in the central nervous system. While oligodendrocyte progenitor cells (OPCs are essential for generating oligodendrocytes for myelin repair, other cell types also participate in the damage and repair processes. The NG2 proteoglycan is expressed by OPCs, pericytes, and macrophages/microglia. In this report we investigate the effects of NG2 on these cell types during spinal cord demyelination/remyelination. Methods Demyelinated lesions were created by microinjecting 1% lysolecithin into the lumbar spinal cord. Following demyelination, NG2 expression patterns in wild type mice were studied via immunostaining. Immunolabeling was also used in wild type and NG2 null mice to compare the extent of myelin damage, the kinetics of myelin repair, and the respective responses of OPCs, pericytes, and macrophages/microglia. Cell proliferation was quantified by studies of BrdU incorporation, and cytokine expression levels were evaluated using qRT-PCR. Results The initial volume of spinal cord demyelination in wild type mice is twice as large as in NG2 null mice. However, over the ensuing 5 weeks there is a 6-fold improvement in myelination in wild type mice, versus only a 2-fold improvement in NG2 null mice. NG2 ablation also results in reduced numbers of each of the three affected cell types. BrdU incorporation studies reveal that reduced cell proliferation is an important factor underlying NG2-dependent decreases in each of the three key cell populations. In addition, NG2 ablation reduces macrophage/microglial cell migration and shifts cytokine expression from a pro-inflammatory to anti-inflammatory phenotype. Conclusions Loss of NG2 expression leads to decreased proliferation of OPCs, pericytes, and macrophages/microglia, reducing the abundance of all three cell types in demyelinated spinal cord lesions. As a result

  11. Processes accompanying of mantle plume emplacement into continental lithosphere: Evidence from NW Arabian plate, Western Syria

    Science.gov (United States)

    Sharkov, E. V.

    2015-12-01

    Lower crustal xenoliths occurred in the Middle Cretaceous lamprophyre diatremes in Jabel Ansaria (Western Syria) (Sharkov et al., 1992). They are represented mainly garnet granulites and eclogite-like rocks, which underwent by deformations and retrograde metamorphism, and younger fresh pegmatoid garnet-kaersutite-clinopyroxene (Al-Ti augite) rocks; mantle peridotites are absent in these populations. According to mineralogical geothermobarometers, forming of garnet-granulite suite rocks occurred under pressure 13.5-15.4 kbar (depths 45-54 kn) and temperature 965-1115oC. At the same time, among populations of mantle xenoliths in the Late Cenozoic platobasalts of the region, quite the contrary, lower crustal xenoliths are absent, however, predominated spinel lherzolites (fragments of upper cooled rim of a plume head), derived from the close depths (30-40 km: Sharkov, Bogatikov, 2015). From this follows that ancient continental crust was existed here even in the Middle Cretaceous, but in the Late Cenozoic was removed by extended mantle plume head; at that upper sialic crust was not involved in geomechanic processes, because Precambrian metamorphic rocks survived as a basement for Cambrian to Cenozoic sedimentary cover of Arabian platform. In other words, though cardinal rebuilding of deep-seated structure of the region occurred in the Late Cenozoic but it did not affect on the upper shell of the ancient lithosphere. Because composition of mantle xenolithis in basalts is practically similar worldwide, we suggest that deep-seated processes are analogous also. As emplacement of the mantle plume heads accompanied by powerful basaltic magmatism, very likely that range of lower (mafic) continental crust existence is very convenient for extension of plume heads and their adiabatic melting. If such level, because of whatever reasons, was not reached, melting was limited but appeared excess of volatile matters which led to forming of lamprophyre or even kimberlite.

  12. A Study of Using Massage Therapy Accompanied with Stretching Exercise for Rehabilitation of Mammary Gland Hyperplasia

    Directory of Open Access Journals (Sweden)

    Pin Lv

    2016-01-01

    Full Text Available Purpose. To apply massage therapy accompanied with stretching exercises for treatment of mammary gland hyperplasia, evaluate the clinical outcome in patients, and estimate the therapy as a novel treatment method for mammary hyperplasia. Methods. 28 adult female patients were selected and treated with massage therapy and stretching exercises focusing on skeleton muscles of chest, abdomen, and axilla. The mammary gland oxyhemoglobin (OxyHb and deoxyhemoglobin (DeoxyHb levels were detected before and after treatment after 15, 30, and 45 days. Results. In this cohort, pretreatment OxyHb (mean ± SD is 1.32±0.14 (medium-high, and DeoxyHb is 0.87±0.13 (normal. All patients were clinically diagnosed with benign mammary gland hyperplasia and mastitis. The posttreatment OxyHb levels are 1.23±0.09 (normal-medium, 15-day, 1.16±0.08 (normal, 30-day, and 1.05±0.04 (normal, 45-day, and DeoxyHb levels are 0.90±0.11 (normal, 15-day, 0.94±0.18 (normal, 30-day, and 0.98±0.12 (normal, 45-day. Patients were diagnosed with decreased hyperplasia 15 and 30 days after treatment and with no symptom of hyperplasia in mammary gland 45 days after treatment. Conclusion. Mammary gland hyperplasia is closely correlated with pathological changes of skeletal muscles and could be significantly improved by massage therapy and stretching exercises targeting neighboring skeletal muscles.

  13. One-way-coupling simulation of cavitation accompanied by high-speed droplet impact

    Science.gov (United States)

    Kondo, Tomoki; Ando, Keita

    2016-03-01

    Erosion due to high-speed droplet impact is a crucial issue in industrial applications. The erosion is caused by the water-hammer loading on material surfaces and possibly by the reloading from collapsing cavitation bubbles that appear within the droplet. Here, we simulate the dynamics of cavitation bubbles accompanied by high-speed droplet impact against a deformable wall in order to see whether the bubble collapse is violent enough to give rise to cavitation erosion on the wall. The evolution of pressure waves in a single water (or gelatin) droplet to collide with a deformable wall at speed up to 110 m/s is inferred from simulations of multicomponent Euler flow where phase changes are not permitted. Then, we examine the dynamics of cavitation bubbles nucleated from micron/submicron-sized gas bubble nuclei that are supposed to exist inside the droplet. For simplicity, we perform Rayleigh-Plesset-type calculations in a one-way-coupling manner, namely, the bubble dynamics are determined according to the pressure variation obtained from the Euler flow simulation. In the simulation, the preexisting bubble nuclei whose size is either micron or submicron show large growth to submillimeters because tension inside the droplet is obtained through interaction of the pressure waves and the droplet interface; this supports the possibility of having cavitation due to the droplet impact. It is also found, in particular, for the case of cavitation arising from very small nuclei such as nanobubbles, that radiated pressure from the cavitation bubble collapse can overwhelm the water-hammer pressure directly created by the impact. Hence, cavitation may need to be accounted for when it comes to discussing erosion in the droplet impact problem.

  14. Investigation of autoimmune diseases accompanying Hashimoto's thyroiditis in children and adolescents and evaluation of cardiac signs.

    Science.gov (United States)

    Baş, Veysel Nijat; Yılmaz Agladioglu, Sebahat; Özgür, Senem; Karademir, Selmin; Aycan, Zehra

    2015-07-01

    In the present study, it was aimed to investigate the concomitance of additional cardiac problems, mainly mitral valve prolapse, in adolescents and pediatric patients with Hashimoto's thyroiditis, by screening autoimmune markers. Fifty-seven euthyroid patients, who applied to the Pediatric Endocrinology clinic at our institution with marked symptoms of hypothyroidism at the time of diagnosis, and were diagnosed and treated for Hashimoto's thyroiditis, were included in the present study. All patients were evaluated by performing non-organ specific autoantibodies which could be tested at our institution, thyroid ultrasonography, two-dimensional echocardiography, and 24-h holter monitorization. Of the 57 cases with Hashimoto's thyroiditis, 48 (84.2%) were female, and nine (15.8%) were male. In the echocardiographic evaluation, mitral valve problems were detected in 10 (17.5%) of all cases; mitral valve prolapse was diagnosed in eight (seven females and one male) cases, and mitral insufficiency was diagnosed in two female cases. First-degree atrioventricular block was observed in only two patients during 24-h holter monitorization. Different non-organ specific autoantibody positivity was distributed as antinuclear antibody in 15 (26.3%) cases, anticardiolipin IgG in two cases, anticardiolipin IgM in three cases, tissue transglutaminase IgA in one, glutamic acid decarboxylase in one, anti-insulin antibody in four cases, antiphospholipid IgG in one, and antiphospholipid IgM in one case. It should be underlined that patients with Hashimoto's thyroiditis should to be followed up closely for mitral valve prolapse and accompanying autoimmune diseases.

  15. Laparoscopic cholecystectomy accompanied by simultaneous umbilical hernia repair: A retrospective study

    Directory of Open Access Journals (Sweden)

    Kamer E

    2007-01-01

    Full Text Available Background : Umbilical defects may cause technical problems for general surgeons in patients during laparoscopic cholecystectomy (LC operations and may increase the incidence of incisional hernia. Aim : The objectives of this study were to determine the optimal repair method for umbilical hernias that already exist or are encountered incidentally and to present data regarding potential problems that may occur during LC. Settings and Design : Medical records of patients who had received simultaneous umbilical hernia repair (UHR with LC were investigated retrospectively. Materials and Methods : Cholelithiasis was accompanied by umbilical hernia in 64 (8.6% out of 745 patients who underwent LC and UHR simultaneously in our hospital between 2000 and 2004. Statistical Analysis Used : The Mann-Whitney U, Chi-square, One-Way Anova, Kaplan-Meier survival analysis, the log-rank test and t test were used for statistical analyses. Results : LC was followed by UHR using primary suture (Group 1, Mayo repair (Group 2 and flat mesh-based repair (Group 3 in 32 (50%, 18 (28.1% and 14 (21.9% patients, respectively. Mean body mass indexes (BMI of patients were 26.6 kg/m 2 , 29.2 kg/m 2 and 39.9 kg/m 2 in Groups 1, 2 and 3, respectively. Recurrence rates were 9.4%, 5.6% and none (0% in Groups 1, 2 and 3, respectively. Recurrence was observed in three (7.0% out of 43(67.2% patients with BMI≥30 kg/m 2 while umbilical hernia recurred in one (4.8% out of 21 (32.8% patients with BMI< 30 kg/m 2 . Overall morbidity and mortality rates were 14.1% and 0%, respectively. Conclusions : The outcomes of the UHR with mesh after laparoscopic surgeries appear to be better for either obese or non-obese patients than primary suture techniques in recurrence rates.

  16. Molecular evolution accompanying functional divergence of duplicated genes along the plant starch biosynthesis pathway.

    Science.gov (United States)

    Nougué, Odrade; Corbi, Jonathan; Ball, Steven G; Manicacci, Domenica; Tenaillon, Maud I

    2014-05-15

    Starch is the main source of carbon storage in the Archaeplastida. The starch biosynthesis pathway (sbp) emerged from cytosolic glycogen metabolism shortly after plastid endosymbiosis and was redirected to the plastid stroma during the green lineage divergence. The SBP is a complex network of genes, most of which are members of large multigene families. While some gene duplications occurred in the Archaeplastida ancestor, most were generated during the sbp redirection process, and the remaining few paralogs were generated through compartmentalization or tissue specialization during the evolution of the land plants. In the present study, we tested models of duplicated gene evolution in order to understand the evolutionary forces that have led to the development of SBP in angiosperms. We combined phylogenetic analyses and tests on the rates of evolution along branches emerging from major duplication events in six gene families encoding sbp enzymes. We found evidence of positive selection along branches following cytosolic or plastidial specialization in two starch phosphorylases and identified numerous residues that exhibited changes in volume, polarity or charge. Starch synthases, branching and debranching enzymes functional specializations were also accompanied by accelerated evolution. However, none of the sites targeted by selection corresponded to known functional domains, catalytic or regulatory. Interestingly, among the 13 duplications tested, 7 exhibited evidence of positive selection in both branches emerging from the duplication, 2 in only one branch, and 4 in none of the branches. The majority of duplications were followed by accelerated evolution targeting specific residues along both branches. This pattern was consistent with the optimization of the two sub-functions originally fulfilled by the ancestral gene before duplication. Our results thereby provide strong support to the so-called "Escape from Adaptive Conflict" (EAC) model. Because none of the

  17. Decreases in community viral load are accompanied by reductions in new HIV infections in San Francisco.

    Directory of Open Access Journals (Sweden)

    Moupali Das

    Full Text Available BACKGROUND: At the individual level, higher HIV viral load predicts sexual transmission risk. We evaluated San Francisco's community viral load (CVL as a population level marker of HIV transmission risk. We hypothesized that the decrease in CVL in San Francisco from 2004-2008, corresponding with increased rates of HIV testing, antiretroviral therapy (ART coverage and effectiveness, and population-level virologic suppression, would be associated with a reduction in new HIV infections. METHODOLOGY/PRINCIPAL FINDINGS: We used San Francisco's HIV/AIDS surveillance system to examine the trends in CVL. Mean CVL was calculated as the mean of the most recent viral load of all reported HIV-positive individuals in a particular community. Total CVL was defined as the sum of the most recent viral loads of all HIV-positive individuals in a particular community. We used Poisson models with robust standard errors to assess the relationships between the mean and total CVL and the primary outcome: annual numbers of newly diagnosed HIV cases. Both mean and total CVL decreased from 2004-2008 and were accompanied by decreases in new HIV diagnoses from 798 (2004 to 434 (2008. The mean (p = 0.003 and total CVL (p = 0.002 were significantly associated with new HIV cases from 2004-2008. CONCLUSIONS/SIGNIFICANCE: Reductions in CVL are associated with decreased HIV infections. Results suggest that wide-scale ART could reduce HIV transmission at the population level. Because CVL is temporally upstream of new HIV infections, jurisdictions should consider adding CVL to routine HIV surveillance to track the epidemic, allocate resources, and to evaluate the effectiveness of HIV prevention and treatment efforts.

  18. [Improvised surgical technique for elderly women with advanced breast cancer accompanied by extensive skin invasion].

    Science.gov (United States)

    Hayashi, Keiko; Kosaka, Yoshimasa; Sengoku, Norihiko; Enomoto, Takumo; Kajita, Sabine; Kuranami, Masaru; Watanabe, Masahiko

    2012-11-01

    Patient 1 was a 63-year-old woman whose chief complaint was a mass in the left breast. Physical examination revealed an inverted left nipple, a very large mass on the anterior aspect of the sternum, and erythema. Because the tumor had directly invaded the sternum, T4cN3M0, stage IIIC breast cancer was diagnosed. The patient preoperatively received chemotherapy with 6 courses of FEC100 (5-fluorouracil, epirubicin, and cyclophosphamide) and 5 courses of nanoparticle albumin -bound paclitaxel (260 mg/m2), which enabled a partial response. Patient 2 was an 83-year-old woman whose chief complaint was a mass in the upper internal and external quadrants of the right breast measuring 20×15 cm and erythema. The mass was accompanied by enlarged right axillary lymph nodes(T4bN1M0, stage IIIB breast cancer). Both patients underwent core needle biopsy of the skin and breast masses. They were both diagnosed with invasive, lobular, triple-negative breast cancer (estrogen receptor negative, progesterone receptor negative, human epidermal growth factor receptor 2 negative). The surgical resection line was drawn to include the extensive skin invasion, and mastectomy and axillary dissection were performed. Skin grafting was scheduled but the retromammary space on the healthy side was dissected to the anterior border of the latissimus dorsi muscle, and the skin of the healthy side was used to cover the defect on the affected side. Consequently, the pendulous breast on the healthy side was elevated. This surgical technique provided an excellent aesthetic outcome without any skin problems, because autologous skin was used to fill the defect. Radiotherapy could subsequently be administered as scheduled. This procedure may be useful for elderly patients.

  19. PTRF/Cavin-1 Deficiency Causes Cardiac Dysfunction Accompanied by Cardiomyocyte Hypertrophy and Cardiac Fibrosis

    Science.gov (United States)

    Ogata, Takehiro; Kasahara, Takeru; Nakanishi, Naohiko; Miyagawa, Kotaro; Naito, Daisuke; Hamaoka, Tetsuro; Nishi, Masahiro; Matoba, Satoaki; Ueyama, Tomomi

    2016-01-01

    Mutations in the PTRF/Cavin-1 gene cause congenital generalized lipodystrophy type 4 (CGL4) associated with myopathy. Additionally, long-QT syndrome and fatal cardiac arrhythmia are observed in patients with CGL4 who have homozygous PTRF/Cavin-1 mutations. PTRF/Cavin-1 deficiency shows reductions of caveolae and caveolin-3 (Cav3) protein expression in skeletal muscle, and Cav3 deficiency in the heart causes cardiac hypertrophy with loss of caveolae. However, it remains unknown how loss of PTRF/Cavin-1 affects cardiac morphology and function. Here, we present a characterization of the hearts of PTRF/Cavin-1-null (PTRF−/−) mice. Electron microscopy revealed the reduction of caveolae in cardiomyocytes of PTRF−/− mice. PTRF−/− mice at 16 weeks of age developed a progressive cardiomyopathic phenotype with wall thickening of left ventricles and reduced fractional shortening evaluated by echocardiography. Electrocardiography revealed that PTRF−/− mice at 24 weeks of age had low voltages and wide QRS complexes in limb leads. Histological analysis showed cardiomyocyte hypertrophy accompanied by progressive interstitial/perivascular fibrosis. Hypertrophy-related fetal gene expression was also induced in PTRF−/− hearts. Western blotting analysis and quantitative RT-PCR revealed that Cav3 expression was suppressed in PTRF−/− hearts compared with that in wild-type (WT) ones. ERK1/2 was activated in PTRF−/− hearts compared with that in WT ones. These results suggest that loss of PTRF/Cavin-1 protein expression is sufficient to induce a molecular program leading to cardiomyocyte hypertrophy and cardiomyopathy, which is partly attributable to Cav3 reduction in the heart. PMID:27612189

  20. Audiovisual aid viewing immediately before pediatric induction moderates the accompanying parents' anxiety.

    Science.gov (United States)

    Berghmans, Johan; Weber, Frank; van Akoleyen, Candyce; Utens, Elisabeth; Adriaenssens, Peter; Klein, Jan; Himpe, Dirk

    2012-04-01

    Parents accompanying their child during induction of anesthesia experience stress. The impact of audiovisual aid (AVA) on parental state anxiety and assessment of the child's anxiety at induction have been studied previously but need closer scrutiny. One hundred and twenty parents whose children were scheduled for day-care surgery entered this randomized, controlled study. The intervention group (n = 60) was exposed to an AVA in the holding area. Parental anxiety was measured with the Spielberger State-Trait Anxiety Inventory and the Amsterdam Preoperative Anxiety and Information Scale (APAIS) at three time points: (i) on admission [T1]; (ii) in the holding area just before entering the operating theater [T2]; and (iii) after leaving [T3]. Additionally, at [T3], both parent and attending anesthetist evaluated the child's anxiety using a visual analogue scale. The anesthetist also filled out the Induction Compliance Checklist. On the state anxiety subscale, APAIS parental anxiety at T2 (P = 0.015) and T3 (P = 0.009) was lower in the AVA intervention group than in the control group. After induction, the child's anxiety rating by the anesthetist was significantly lower than by the parent, in both intervention and control groups. Preoperative AVA shown to parents immediately before induction moderates the increase in anxiety associated with the anesthetic induction of their child. Present results suggest that behavioral characteristics seem better predictors of child's anxiety during induction than anxiety ratings per se and that anesthetists are better than parents in predicting child's anxiety during induction. © 2011 Blackwell Publishing Ltd.

  1. Hybridization accompanying FRET event in labeled natural nucleoside-unnatural nucleoside containing chimeric DNA duplexes.

    Science.gov (United States)

    Bag, Subhendu Sekhar; Das, Suman K; Pradhan, Manoj Kumar; Jana, Subhashis

    2016-09-01

    Förster resonance energy transfer (FRET) is a highly efficient strategy in illuminating the structures, structural changes and dynamics of DNA, proteins and other biomolecules and thus is being widely utilized in studying such phenomena, in designing molecular/biomolecular probes for monitoring the hybridization event of two single stranded DNA to form duplex, in gene detection and in many other sensory applications in chemistry, biology and material sciences. Moreover, FRET can give information about the positional status of chromophores within the associated biomolecules with much more accuracy than other methods can yield. Toward this end, we want to report here the ability of fluorescent unnatural nucleoside, triazolylphenanthrene ((TPhen)BDo) to show FRET interaction upon hybridization with fluorescently labeled natural nucleosides, (Per)U or (OxoPy)U or (Per)U, forming two stable chimeric DNA duplexes. The pairing selectivity and the thermal duplex stability of the chimeric duplexes are higher than any of the duplexes with natural nucleoside formed. The hybridization results in a Förster resonance energy transfer (FRET) from donor triazolylphenanthrene of (TPhen)BDo to acceptor oxopyrene of (OxoPy)U and/or to perylene chromophore of (Per)U, respectively, in two chimeric DNA duplexes. Therefore, we have established the FRET process in two chimeric DNA duplexes wherein a fluorescently labeled natural nucleoside ((OxoPy)U or (Per)U) paired against an unnatural nucleoside ((TPhen)BDo) without sacrificing the duplex stability and B-DNA conformation. The hybridization accompanying FRET event in these classes of interacting fluorophores is new. Moreover, there is no report of such designed system of chimeric DNA duplex. Our observed phenomenon and the design can potentially be exploited in designing more of such efficient FRET pairs for useful application in the detection and analysis of biomolecular interactions and in material science application. Copyright

  2. Movement-related potentials accompanying unilateral and bilateral finger movements with different inertial loads.

    Science.gov (United States)

    Kristeva, R; Cheyne, D; Lang, W; Lindinger, G; Deecke, L

    1990-05-01

    The present study was aimed at investigating the effect of inertial loading on movement-related potentials (MRPs) recorded from the scalps of normal subjects while performing finger movements. Two experiments were performed. Experiment 1. MRPs preceding and accompanying the execution of voluntary, unilateral finger movements were investigated in 8 subjects under the 3 experimental conditions of: no inertial load, small inertial load (250 g), and large inertial load (400 g). A significant effect of the inertial load on Bereitschaftspotential (BP) amplitude was observed for the 100 msec period preceding movement onset (BP -100 to 0) at precentral electrode sites and following movement onset (N0 to 100) at both precentral and parietal electrode sites. Pairwise comparisons revealed that significant effects were due to differences between the loading and non-loading conditions and not for different amounts of loading. No significant differences were observed for BP onset or early BP amplitudes, indicating that scalp negativity immediately prior to, and during, movement onset is primarily influenced by conditions of inertial loading. Experiment 2. This experiment examined the effect of inertial loading on MRPs for bilateral, simultaneous voluntary finger movements in 10 subjects under conditions of: no inertial load, inertial load applied separately to the left and right fingers, and with identical inertial loads applied to both fingers. No significant effect of inertial load on MRP amplitude was observed. These results are contrasted with those of experiment 1 which show significant effects of inertial loading for unilateral movements and are interpreted in terms of the hypothesis that bilateral movement organization involves 'higher' aspects of motor control than those reflecting adjustment to conditions of inertial loading.

  3. Genome-wide changes accompanying knockdown of fatty acid synthase in breast cancer

    Directory of Open Access Journals (Sweden)

    Smith Jeffrey W

    2007-06-01

    Full Text Available Abstract Background The lipogenic enzyme fatty acid synthase (FAS is up-regulated in a wide variety of cancers, and is considered a potential metabolic oncogene by virtue of its ability to enhance tumor cell survival. Inhibition of tumor FAS causes both cell cycle arrest and apoptosis, indicating FAS is a promising target for cancer treatment. Results Here, we used gene expression profiling to conduct a global study of the cellular processes affected by siRNA mediated knockdown of FAS in MDA-MB-435 mammary carcinoma cells. The study identified 169 up-regulated genes (≥ 1.5 fold and 110 down-regulated genes (≤ 0.67 fold in response to knockdown of FAS. These genes regulate several aspects of tumor function, including metabolism, cell survival/proliferation, DNA replication/transcription, and protein degradation. Quantitative pathway analysis using Gene Set Enrichment Analysis software further revealed that the most pronounced effect of FAS knockdown was down-regulation in pathways that regulate lipid metabolism, glycolysis, the TCA cycle and oxidative phosphorylation. These changes were coupled with up-regulation in genes involved in cell cycle arrest and death receptor mediated apoptotic pathways. Conclusion Together these findings reveal a wide network of pathways that are influenced in response to FAS knockdown and provide new insight into the role of this enzyme in tumor cell survival and proliferation.

  4. Voice and speech outcomes with radial forearm free flap-accompanied phonation tube after total pharyngolaryngectomy of hypopharyngeal cancer.

    Science.gov (United States)

    Yang, Cheng-Chien; Lee, Jehn-Chuan; Wu, Kang-Chao; Chang, Shih-Hsin

    2011-08-01

    Radial forearm free flap (RFFF)-accompanied phonation tube (PT) for voice and speech restoration after pharyngolaryngectomy is promising, especially in phonation efficacy and intelligibility. It offers not only another safe surgical option but also a satisfactory result for such patients with advanced hypopharyngeal cancer. We use RFFF with RFFF-accompanied PT for one-stage reconstruction both for tissue defect and voice reconstruction in patients undergoing total pharyngolaryngectomy. Eight male patients with advanced hypopharyngeal cancer underwent total pharyngolaryngectomy. Voice restoration was done with RFFF-accompanied PT. Phonation outcomes and speech outcomes of the patients were evaluated and scored. The mean follow-up time was 13.7 months. All free flaps were successful without perioperative mortality. All the patients were able to produce sound. Phonation efficacy ranged from 70% to >90% postoperatively and 40% to >90% at the last follow-up. The speech intelligibility was graded as moderately good.

  5. Steroid-responsive thalamic lesions accompanying microbleeds in a case of Hashimoto's encephalopathy with autoantibodies against α-enolase.

    Science.gov (United States)

    Yamaguchi, Yoshitaka; Wada, Manabu; Tanji, Haruko; Kurokawa, Katsuro; Kawanami, Toru; Tanji, Kazuyo; Yoneda, Makoto; Kato, Takeo

    2013-01-01

    A 67-year-old man receiving antithrombotic therapy developed rapidly progressive amnesia. T2-weighted images of brain MRI revealed hyperintense lesions in the bilateral thalami accompanied by microbleeds. Antithyroglobulin antibodies and autoantibodies against the N-terminal of α-enolase (NAE) were identified in the patient's serum; therefore, Hashimoto's encephalopathy (HE) was suspected. Although the patient's radiological findings improved following steroid therapy, his symptoms did not improve, possibly due to increased thalamic microbleeds. Because anti-NAE antibodies are possibly associated with vasculitis, HE accompanied by anti-NAE antibodies may be exacerbated by microbleeds in patients receiving antithrombotic therapy.

  6. The Comparison of the Effect of Block Flute Accompanied Song Teaching with Multi-Sound Notation and Vocalization Program Accompanied Song Teaching on the Success of Students' Song Learning Behavior

    Science.gov (United States)

    Saktanli, S. Cem

    2011-01-01

    This experimental study was done to see if using computer supported notation and vocalization program for teaching songs instead of using block flute accompanied song teaching has any significant effect on students' singing behavior. The study group is composed of the 5th, 6th and 7th graders of 2008-2009 educational term in T.O.K.I. Yahya Kemal…

  7. DNA methylation changes in plasticity genes accompany the formation and maintenance of memory.

    Science.gov (United States)

    Halder, Rashi; Hennion, Magali; Vidal, Ramon O; Shomroni, Orr; Rahman, Raza-Ur; Rajput, Ashish; Centeno, Tonatiuh Pena; van Bebber, Frauke; Capece, Vincenzo; Garcia Vizcaino, Julio C; Schuetz, Anna-Lena; Burkhardt, Susanne; Benito, Eva; Navarro Sala, Magdalena; Javan, Sanaz Bahari; Haass, Christian; Schmid, Bettina; Fischer, Andre; Bonn, Stefan

    2016-01-01

    The ability to form memories is a prerequisite for an organism's behavioral adaptation to environmental changes. At the molecular level, the acquisition and maintenance of memory requires changes in chromatin modifications. In an effort to unravel the epigenetic network underlying both short- and long-term memory, we examined chromatin modification changes in two distinct mouse brain regions, two cell types and three time points before and after contextual learning. We found that histone modifications predominantly changed during memory acquisition and correlated surprisingly little with changes in gene expression. Although long-lasting changes were almost exclusive to neurons, learning-related histone modification and DNA methylation changes also occurred in non-neuronal cell types, suggesting a functional role for non-neuronal cells in epigenetic learning. Finally, our data provide evidence for a molecular framework of memory acquisition and maintenance, wherein DNA methylation could alter the expression and splicing of genes involved in functional plasticity and synaptic wiring.

  8. Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in Arabidopsis thaliana.

    Science.gov (United States)

    Hossain, Mohammad Shakhawat; Ye, Wenxiu; Hossain, Mohammad Anowar; Okuma, Eiji; Uraji, Misugi; Nakamura, Yoshimasa; Mori, Izumi C; Murata, Yoshiyuki

    2013-01-01

    Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca(2+)]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca(2+)]cyt oscillation in Arabidopsis.

  9. Status of clean vehicle technologies and impact of the accompanying public policies; Etat des filieres de vehicules propres et impact des politiques publiques d'accompagnement

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-06-01

    The French inter-ministry committee for clean vehicles (CIVP) published in 1999 a report about the status of development of the different clean vehicle technologies (electric-powered, LPG-fueled, natural gas fueled, hybrid, fuel cells) and accompanied by recommendations for public policies. A re-evaluation of these technologies was planned by the end of 2002 and is the purpose of this document. The first part makes a status of the actions carried out by the public authorities since the previous CIVP report. The second part presents the present day situation of the LPG, natural gas and electric technologies. It describes also the recent advances in the classical technologies (gasoline and diesel engines) and includes a part about battery technologies and about the use of bio-fuels. The status of each technology is presented both for France and for foreign countries and with its perspectives of evolution. (J.S.)

  10. Age-related changes in cerebellar and hypothalamic function accompany non-microglial immune gene expression, altered synapse organization, and excitatory amino acid neurotransmission deficits

    Science.gov (United States)

    Bonasera, Stephen J.; Arikkath, Jyothi; Boska, Michael D.; Chaudoin, Tammy R.; DeKorver, Nicholas W.; Goulding, Evan H.; Hoke, Traci A.; Mojtahedzedah, Vahid; Reyelts, Crystal D.; Sajja, Balasrinivasa; Schenk, A. Katrin; Tecott, Laurence H.; Volden, Tiffany A.

    2016-01-01

    We describe age-related molecular and neuronal changes that disrupt mobility or energy balance based on brain region and genetic background. Compared to young mice, aged C57BL/6 mice exhibit marked locomotor (but not energy balance) impairments. In contrast, aged BALB mice exhibit marked energy balance (but not locomotor) impairments. Age-related changes in cerebellar or hypothalamic gene expression accompany these phenotypes. Aging evokes upregulation of immune pattern recognition receptors and cell adhesion molecules. However, these changes do not localize to microglia, the major CNS immunocyte. Consistent with a neuronal role, there is a marked age-related increase in excitatory synapses over the cerebellum and hypothalamus. Functional imaging of these regions is consistent with age-related synaptic impairments. These studies suggest that aging reactivates a developmental program employed during embryogenesis where immune molecules guide synapse formation and pruning. Renewed activity in this program may disrupt excitatory neurotransmission, causing significant behavioral deficits. PMID:27689748

  11. A multilocus perspective on colonization accompanied by selection and gene flow.

    Science.gov (United States)

    Rosenblum, Erica Bree; Hickerson, Michael J; Moritz, Craig

    2007-12-01

    initial bottleneck. Both ABC analyses and measures of mtDNA sequence diversity also suggested that population reductions were more severe in the black lava compared to the white sands habitat. Differences observed between habitats may be explained by differences in colonization time, habitat geometry, and strength or response to natural selection for substrate matching. Finally, effective population size reductions in this system appear to be more dramatic when colonization is accompanied by a change in selection regime. Our analyses are consistent with a demographic cost of adaptation to novel environments and show that it is possible to infer aspects of the historical demography of local adaptation even in the presence of ongoing gene flow.

  12. [Family companions. New facets in accompanying relatives caring for people with dementia].

    Science.gov (United States)

    Kricheldorff, Cornelia; Brijoux, Thomas

    2016-04-01

    qualified and accompanied at least 73 families in rural areas, which was the special focus of the model project. The developed qualification enabled the volunteers to carry out the activities in a very relationship-oriented way and in a direct form of communication with the family carers and also to encourage self-care activities. This led to a significant improvement in the health-related quality of life (QoL) of family carers and stabilized the homecare setting. As part of a health economic model calculation on the effects of the new approach, the pecuniary gains outweighed the costs caused by the qualification. The concept of family companions (Familienbegleiter) has proven to be a cost-covering model of an effective intervention based on the needs of caring families, which should become more widespread in the future.

  13. Nodular intra-abdominal panniculitis: an accompaniment of colorectal carcinoma and diverticular disease

    DEFF Research Database (Denmark)

    Bak, Martin

    1996-01-01

    should not be regarded as a specific nosological entity but merely a result of injury to the fat cells. Intra-abdominal panniculitis is seen more often as a secondary local phenomenon than as a primary condition, and in both cases it is associated with considerable differential diagnostic problems...

  14. Career & Life Planning Portfolio Resource Book. Activities To Accompany the South Dakota Career and Life Planning Portfolio, 1999-2000.

    Science.gov (United States)

    South Dakota State Dept. of Education, Pierre. Div. of Workforce and Career Preparation.

    This resource book provides several career development activities to accompany the South Dakota "My Career and Life Planning Portfolio," which is appended. The activities are arranged according to the following four folders found within the portfolio jacket: career and education planning; skills employers want; projects/work samples; and…

  15. A comparison of information on clouds and phenomena accompanying Cb obtained from surface and radar net stations

    Science.gov (United States)

    Gashina, S. B.; Matveyeva, L. I.; Razumovskiy, A. A.

    1975-01-01

    Methods and criteria are proposed for the comparison of surface and radar data on clouds and phenomena accompanying Cb. It is shown that the effectiveness of the radar information on thunderstorms from a net over the territory of the BSSR is approximately 3 times greater than that of visual observations obtained from the station net.

  16. 30 CFR 250.260 - What Coastal Zone Management Act (CZMA) information must accompany the DPP or DOCD?

    Science.gov (United States)

    2010-07-01

    ...) information must accompany the DPP or DOCD? 250.260 Section 250.260 Mineral Resources MINERALS MANAGEMENT... Operations Coordination Documents (docd) § 250.260 What Coastal Zone Management Act (CZMA) information must.... “Information” as required by 15 CFR 930.76(a) and 15 CFR 930.58(a)(2)) and “Analysis” as required by 15 CFR...

  17. Understanding Pretend Play. A Guide for Parents and Teachers To Accompany the Documentary Video "When a Child Pretends."

    Science.gov (United States)

    Drucker, Jan; Franklin, Margery B.; Wilford, Sara

    Pretend play is often undervalued and ignored. This videotape and accompanying booklet highlight how the dramatic scenarios, microworlds, storytelling, and block building of pretend play provide young children the opportunity to develop skills for a lifetime of intellectual, social, emotional, and creative development. The booklet describes the…

  18. Accompany: Acceptable robotiCs COMPanions for AgeiNG Years — Multidimensional aspects of human-system interactions

    NARCIS (Netherlands)

    Amirabdollahian, F.; op den Akker, R.; Bedaf, S.; Bormann, R.; Draper, H.; Evers, V.; Gelderblom, G.J.; Gutierrez Ruiz, C.; Hewson, D.; Hu, N.; Iacono, I.; Koay, K.L.; Kröse, B.; Marti, P.; Michel, H.; Prevot-Huille, H.; Reiser, U.; Saunders, J.; Sorell, T.; Dautenhahn, K.; Paja, W.A.; Wilamowski, B.M.

    2013-01-01

    Email Print Request Permissions Save to Project With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European

  19. ACCOMPANY: ACceptable robotiCs COMPanions for AgeiNg Years: multidimensional aspects of human-system interactions

    NARCIS (Netherlands)

    Amirabdollahian, Farshid; op den Akker, Hendrikus J.A.; Bedaf, Sandra; Bormann, Richard; Draper, Heather; Gelderblom, Gert Jan; Evers, Vanessa; Gutierrez Ruiz, Carolina; Hewson, David; Iacono, Iolanda; Koay, Kheng Lee; Krose, Ben; Marti, Patrizia; Prevot-Huille, Helene; Reiser, Ulrich; Saunders, Joe; Sorell, Tom; Dautenhahn, Kerstin

    2013-01-01

    With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European project which focuses on home companion

  20. The Contribution of Conceptual Change Texts Accompanied by Concept Mapping to Eleventh-Grade Students Understanding of Cellular Respiration Concepts

    Science.gov (United States)

    Al khawaldeh, Salem A.; Al Olaimat, Ali M.

    2010-01-01

    The present study conducted to investigate the contribution of conceptual change texts, accompanied by concept mapping instruction to eleventh-grade students' understanding of cellular respiration concepts, and their retention of this understanding. Cellular respiration concepts test was developed as a result of examination of related literature…

  1. Teacher Education Students' Perceptions of the Value of Handouts Accompanying Teacher Educators' Computer-Generated Slide Presentations

    Science.gov (United States)

    Yilmazel-Sahin, Yesim; Oxford, Rebecca L.

    2010-01-01

    This mixed-methods study used interviews and a questionnaire to investigate the perceptions of 304 teacher education students regarding the learning-related value of handouts accompanying teacher educators' computer-generated slide presentations. The extent to which graduate and undergraduate students differed in their perceptions was also…

  2. Accompany: Acceptable robotiCs COMPanions for AgeiNG Years — Multidimensional aspects of human-system interactions

    NARCIS (Netherlands)

    F. Amirabdollahian; R. op den Akker; S. Bedaf; R. Bormann; H. Draper; V. Evers; G.J. Gelderblom; C. Gutierrez Ruiz; D. Hewson; N. Hu; I. Iacono; K.L. Koay; B. Kröse; P. Marti; H. Michel; H. Prevot-Huille; U. Reiser; J. Saunders; T. Sorell; K. Dautenhahn

    2013-01-01

    Email Print Request Permissions Save to Project With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European p

  3. ACCOMPANY: ACceptable robotiCs COMPanions for AgeiNg Years: multidimensional aspects of human-system interactions

    NARCIS (Netherlands)

    Amirabdollahian, Farshid; Akker, op den Rieks; Bedaf, Sandra; Bormann, Richard; Draper, Heather; Gelderblom, Gert Jan; Gutierrez Ruiz, Carolina; Hewson, David; Iacono, Iolanda; Koay, Kheng Lee; Krose, Ben; Marti, Patrizia; Prevot-Huille, Helene; Reiser, Ulrich; Saunders, Joe; Sorell, Tom; Dautenhahn, Kerstin

    2013-01-01

    With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European project which focuses on home companion technolog

  4. Limitation of activities of daily living accompanying reduced neck mobility after laminoplasty preserving or reattaching the semispinalis cervicis into axis.

    Science.gov (United States)

    Takeuchi, Kazunari; Yokoyama, Toru; Ono, Atsushi; Numasawa, Takuya; Wada, Kanichiro; Itabashi, Taito; Toh, Satoshi

    2008-03-01

    Although difficulties with neck mobility often interfere with patients' activities of daily living (ADL) after cervical laminoplasty, there was no detailed study on the relation between the limitations of ADL accompanying postoperative reduced neck mobility and the cervical posterior approach. The aim of this study was to compare retrospectively the frequency of limitations of ADL accompanying neck mobility after laminoplasty preserving the semispinalis cervicis inserted into the C2 spinous process with that after laminoplasty reattaching the muscle to C2. Forty-nine patients after C4-C7 laminoplasty with C3 laminectomy preserving the semispinalis cervicis inserted into C2 (Group A) and 24 patients after C3-C7 laminoplasty reattaching the muscle (Group B) were evaluated. The frequency of postoperative limitations of ADL accompanying each of three neck movements of extension, flexion and rotation were investigated. The postoperative O-C7 angles at extension and flexion was measured on lateral extension and flexion radiographs of the cervical spine, respectively. The postoperative cervical range of motion in rotation was measured in the cranial view using a digital camera. Frequency of limitations of ADL accompanying extension was lower (P = 0.037) in Group A (2%) than in Group B (17%). Frequency of limitations of ADL accompanying flexion was similar in Group A (8%) and Group B (4%). Frequency of limitations of ADL accompanying rotation was lower (P = 0.031) in Group A (12%) than in Group B (33%). Average O-C7 angle at extension was significantly larger (P = 0.002) in Group A (147 degrees ) than in Group B (136 degrees ). Average O-C7 angle at flexion was similar in Group A (93 degrees ) and Group B (91 degrees ). Average range of motion in rotation was significantly larger (P = 0.004) in Group A (110 degrees ) than in Group B (91 degrees ). This retrospective study suggested that the frequency of limitations of ADL accompanying neck extension or rotation was lower

  5. Apoptosis, mastocytosis, and diminished adipocytokine gene expression accompany reduced epididymal fat mass in long-standing diet-induced obese mice

    Directory of Open Access Journals (Sweden)

    Altintas Mehmet M

    2011-11-01

    Full Text Available Abstract Background Obesity is characterized by increased cell death and inflammatory reactions in the adipose tissue. Here, we explored pathophysiological alterations taking place in the adipose tissue in long-standing obesity. In the epididymal fat of C57BL/6 mice fed a high-fat diet for 20 weeks, the prevalence and distribution of dead adipocytes (crown-like structures, mast cells (toluidine blue, mMCP6, macrophages (F4/80, and apoptotic cells (cleaved caspase-3 were measured. Moreover, gene and/or protein expression of several adipocytokines (leptin, adiponectin, TNF-α, IL-10, IL-6, MCP-1, F4/80, mMCP6, cleaved caspase-3 were determined. Results We observed that the epididymal fat mass was lower in obese than in lean mice. In obese mice, the epididymal fat mass correlated inversely with body weight and liver mass. Dead adipocytes, mast cells, macrophages, and apoptotic cells were abundant in the epididymal fat of obese mice, especially in the rostral vs. caudal zone. Accordingly, mMCP6, F4/80, and cleaved caspase-3 gene and/or protein expression was increased. Conversely, adiponectin, leptin, IL-6, and MCP-1 gene expression levels were lower in the epididymal fat of obese than lean mice. Although TNF-α and IL-10 gene expression was higher in the epididymal fat of obese mice, their expression relative to F4/80 and mMCP6 expression were lower in the heavily infiltrated rostral than caudal zone. Conclusions This study demonstrates that in mice with long-standing obesity diminished gene expression of several adipocytokines accompany apoptosis and reduced mass of the epididymal fat. Our findings suggest that this is due to both increased prevalence of dead adipocytes and altered immune cell activity. Differential distribution of metabolically challenged adipocytes is indicative of the presence of biologically diverse zones within the epididymal fat.

  6. Clinical value of plasma B-type natriuretic peptide assay in pediatric pneumonia accompanied by heart failure

    Science.gov (United States)

    HU, DAN; LIU, YANG; TAO, HUIXIAN; GAO, JINPING

    2015-01-01

    Previous studies have shown that B-type natriuretic peptide (BNP) is useful in differentiating cardiac from pulmonary causes of dyspnea in adults. To date, international guidelines have recommended measurements of circulating BNP as a biomarker for diagnostic and prognostic purposes, as well as therapeutic monitoring, in adults with cardiac diseases, particularly those suffering from acute and chronic heart failure (HF). The aim of the present study was to investigate the differential diagnostic and therapeutic analysis of BNP levels assayed in pediatric pneumonia accompanied by HF. The clinical data of 80 patients with pneumonia, aged 1–3 years, were analyzed. The patients were divided into two groups: Simple pneumonia (46 cases) and pneumonia accompanied by HF (34 cases). All patients underwent two plasma BNP assays: The first one upon admission to the hospital and the second one prior to discharge. The plasma BNP levels of 20 healthy children were used as the negative control. Plasma BNP levels were measured using the Triage® BNP automated immunoassay systems and reagents. Statistical analysis showed that the plasma BNP levels of the patients upon admission were higher in the pneumonia accompanied by HF group compared with those in the simple pneumonia group (750±120 vs. 135±50 pg/ml; P<0.05). In addition, in the pneumonia accompanied by HF group, the plasma BNP levels of the patients were higher upon admission to the hospital than they were prior to discharge (750±120 vs. 115±45 pg/ml; P<0.05); therefore, plasma BNP may comprise a sensitive diagnostic and therapeutic evaluative marker for pediatric patients with pneumonia accompanied by HF. This finding could prove invaluable in the clinical diagnosis and treatment of the disease. PMID:26668612

  7. Phosphoproteome analysis of streptomyces development reveals extensive protein phosphorylation accompanying bacterial differentiation

    DEFF Research Database (Denmark)

    Manteca, Angel; Ye, Juanying; Sánchez, Jesús

    2011-01-01

    Streptomycetes are bacterial species that undergo a complex developmental cycle that includes programmed cell death (PCD) events and sporulation. They are widely used in biotechnology because they produce most clinically relevant secondary metabolites. Although Streptomyces coelicolor is one...... events were detected during the presporulation and sporulation stages (80%). Most of these phosphorylations were not reported before in Streptomyces, and included sporulation factors, transcriptional regulators, protein kinases and other regulatory proteins. Several of the identified phosphorylated...

  8. Myeloid-Specific Blockade of Notch Signaling by RBP-J Knockout Attenuates Spinal Cord Injury Accompanied by Compromised Inflammation Response in Mice.

    Science.gov (United States)

    Chen, Bei-Yu; Zheng, Min-Hua; Chen, Yan; Du, Yan-Ling; Sun, Xiao-Long; Zhang, Xing; Duan, Li; Gao, Fang; Liang, Liang; Qin, Hong-Yan; Luo, Zhuo-Jing; Han, Hua

    2015-12-01

    The outcome of spinal cord injury (SCI) is determined by both neural cell-intrinsic survival pathways and tissue microenvironment-derived signals. Macrophages dominating the inflammatory responses in SCI possess both destructive and reparative potentials, according to their activation status. Notch signaling is involved in both cell survival and macrophage-mediated inflammation, but a comprehensive role of Notch signaling in SCI has been elusive. In this study, we compared the effects of general Notch blockade by a pharmaceutical γ-secretase inhibitor (GSI) and myeloid-specific Notch signal disruption by recombination signal binding protein Jκ (RBP-J) knockout on SCI. The administration of Notch signal inhibitor GSI resulted in worsened hind limb locomotion and exacerbated inflammation. However, mice lacking RBP-J, the critical transcription factor mediating signals from all four mammalian Notch receptors, in myeloid lineage displayed promoted functional recovery, attenuated glial scar formation, improved neuronal survival and axon regrowth, and mitigated inflammatory response after SCI. These benefits were accompanied by enhanced AKT activation in the lesion area after SCI. These findings demonstrate that abrogating Notch signal in myeloid cells ameliorates inflammation response post-SCI and promotes functional recovery, but general pharmaceutical Notch interception has opposite effects. Therefore, clinical intervention of Notch signaling in SCI needs to pinpoint myeloid lineage to avoid the counteractive effects of global inhibition.

  9. Aquaporin-1 Facilitates Angiogenic Invasion in the Pathologic Neovasculature that Accompanies Cirrhosis

    Science.gov (United States)

    Huebert, Robert C.; Vasdev, Meher M.; Shergill, Uday; Das, Amitava; Huang, Bing Q.; Charlton MR, Michael R.; LaRusso, Nicholas F.; Shah, Vijay H.

    2010-01-01

    Increasing evidence suggests that hepatic fibrosis and pathologic angiogenesis are inter-dependent processes that occur in parallel. Endothelial cell invasion is requisite for angiogenesis and thus studies of the mechanisms governing liver endothelial cell (LEC) invasion during cirrhosis are of great importance. Emerging research implicates amoeboid-type motility and membrane blebbing as features that may facilitate invasion through matrix-rich microenvironments. Aquaporins (AQPs) are integral membrane water channels, recognized for their importance in epithelial secretion and absorption. However, recent studies also suggest links between water transport and cell motility / invasion. Therefore, the purpose of this study was to test the hypothesis that AQP-1 is involved in amoeboid motility and angiogenic invasion during cirrhosis. AQP-1 expression and localization was examined in normal and cirrhotic liver tissues derived from human and mouse. AQP-1 levels were modulated in LEC using retroviral overexpression or siRNA knockdown and functional effects on invasion, membrane blebbing dynamics, and osmotic water permeability were assayed. Results demonstrate that AQP-1 is up-regulated in the small, angiogenic, neo-vasculature within the fibrotic septa of cirrhotic liver. AQP-1 overexpression promotes FGF-induced dynamic membrane blebbing in LEC which is sufficient to augment invasion through extracellular matrix. Additionally, AQP-1 localizes to plasma membrane blebs where it increases osmotic water permeability and locally facilitates the rapid, trans-membrane flux of water. CONCLUSION AQP-1 enhances osmotic water permeability and FGF-induced dynamic membrane blebbing in LEC and thereby drives invasion and pathologic angiogenesis during cirrhosis PMID:20578142

  10. Bacterial Pericarditis Accompanied by Sudden Cardiac Tamponade After Transbronchial Needle Aspiration Cytology.

    Science.gov (United States)

    Matsumoto, Takeshi; Otsuka, Kojiro; Imai, Yukihiro; Tomii, Keisuke

    2016-04-01

    A 48-year-old man was referred for an abnormal shadow in his chest x-ray. Transbronchial needle aspiration cytology was performed at the mediastinal necrotic lymph node #7, and he was diagnosed as having small cell carcinoma. Fifteen days after bronchoscopy, sudden cardiac tamponade occurred and pericardial drainage suggested a diagnosis of bacterial pericarditis. He was successfully treated by drainage and administration of antibiotics. Complication of bacterial pericarditis associated with bronchoscopy is rare. However, physicians should watch for the appearance of this condition for up to 3 weeks after bronchoscopy, especially in cases with necrotic lymph nodes.

  11. Nodular intra-abdominal panniculitis: an accompaniment of colorectal carcinoma and diverticular disease

    DEFF Research Database (Denmark)

    Bak, Martin

    1996-01-01

    was undertaken in order to describe the entire spectrum of the disease including primary as well as secondary cases. Eleven patients are reported, nine of which had an associated colorectal disease in direct continuity with areas of intra-abdominal panniculitis. It is concluded that intra-abdominal panniculitis...... should not be regarded as a specific nosological entity but merely a result of injury to the fat cells. Intra-abdominal panniculitis is seen more often as a secondary local phenomenon than as a primary condition, and in both cases it is associated with considerable differential diagnostic problems...

  12. On the National Characteristics of the Piano Accompani-ment Music by Li Yinghai%黎英海钢琴伴奏音乐的民族特色

    Institute of Scientific and Technical Information of China (English)

    陈芳

    2014-01-01

    Mr. Li Yinghai is a celebrated composer and educator in Chinese modern and contemporary history of music, and his piano accompaniment music is of unique and distinctive artistic characteristics. This paper mainly analyzes the national charac-teristics of the piano accompaniment music by Li Yinghai, striv-ing to explore the characteristic of referring to Western composing techniques and absorbing Chinese national music elements in Mr. Li Yinghai's creation of piano accompaniment music.%黎英海先生是中国近现代音乐史上的著名作曲家、教育家,在他的钢琴创作领域中,钢琴伴奏音乐独具鲜明的艺术特色。本文主要对黎英海先生钢琴伴奏音乐的民族特色进行分析,力求对黎英海先生钢琴伴奏音乐创作在借鉴西方作曲技法与吸取中国民族音乐语言上的特点进行探究。

  13. Dynamic deposition of histone variant H3.3 accompanies developmental remodeling of the Arabidopsis transcriptome.

    Directory of Open Access Journals (Sweden)

    Heike Wollmann

    Full Text Available In animals, replication-coupled histone H3.1 can be distinguished from replication-independent histone H3.3. H3.3 variants are enriched at active genes and their promoters. Furthermore, H3.3 is specifically incorporated upon gene activation. Histone H3 variants evolved independently in plants and animals, and it is unclear whether different replication-independent H3.3 variants developed similar properties in both phyla. We studied Arabidopsis H3 variants in order to find core properties of this class of histones. Here we present genome-wide maps of H3.3 and H3.1 enrichment and the dynamic changes of their profiles upon cell division arrest. We find H3.3 enrichment to positively correlate with gene expression and to be biased towards the transcription termination site. In contrast with H3.1, heterochromatic regions are mostly depleted of H3.3. We report that, in planta, dynamic changes in H3.3 profiles are associated with the extensive remodeling of the transcriptome that occurs during cell differentiation. We propose that H3.3 dynamics are linked to transcription and are involved in resetting covalent histone marks at a genomic scale during plant development. Our study suggests that H3 variants properties likely result from functionally convergent evolution.

  14. Axonal loss and neurofilament phosphorylation changes accompany lesion development and clinical progression in multiple sclerosis.

    Science.gov (United States)

    Schirmer, Lucas; Antel, Jack P; Brück, Wolfgang; Stadelmann, Christine

    2011-07-01

    Neuroaxonal damage and loss are increasingly recognized as disability determining features in multiple sclerosis (MS) pathology. However, little is known about the long-term sequelae of inflammatory demyelination on neurons and axons. Spinal cord tissue of 31 MS patients was compared to three amyotrophic lateral sclerosis (ALS) and 10 control subjects. MS lesions were staged according to the density of KiM-1P positive macrophages and microglia and the presence of myelin basic protein (MBP) positive phagocytes. T cells were quantified in the parenchyma and meninges. Neuroaxonal changes were studied by immunoreactivity (IR) for amyloid precursor protein (APP) and variably phosphorylated neurofilaments (SMI312, SMI31, SMI32). Little T cell infiltration was still evident in chronic inactive lesions. The loss of SMI32 IR in ventral horn neurons correlated with MS lesion development and disease progression. Similarly, axonal loss in white matter (WM) lesions correlated with disease duration. A selective reduction of axonal phosphorylated neurofilaments (SMI31) was observed in WM lesions. In ALS, the loss of neuronal SMI32 IR was even more pronounced, whereas the relative axonal reduction resembled that found in MS. Progressive neuroaxonal neurofilament alterations in the context of chronic inflammatory demyelination may reflect changes in neuroaxonal metabolism and result in chronic neuroaxonal dysfunction as a putative substrate of clinical progression.

  15. Dynamic Alterations to α-Actinin Accompanying Sarcomere Disassembly and Reassembly during Cardiomyocyte Mitosis.

    Science.gov (United States)

    Fan, Xiaohu; Hughes, Bryan G; Ali, Mohammad A M; Cho, Woo Jung; Lopez, Waleska; Schulz, Richard

    2015-01-01

    Although mammals are thought to lose their capacity to regenerate heart muscle shortly after birth, embryonic and neonatal cardiomyocytes in mammals are hyperplastic. During proliferation these cells need to selectively disassemble their myofibrils for successful cytokinesis. The mechanism of sarcomere disassembly is, however, not understood. To study this, we performed a series of immunofluorescence studies of multiple sarcomeric proteins in proliferating neonatal rat ventricular myocytes and correlated these observations with biochemical changes at different cell cycle stages. During myocyte mitosis, α-actinin and titin were disassembled as early as prometaphase. α-actinin (representing the sarcomeric Z-disk) disassembly precedes that of titin (M-line), suggesting that titin disassembly occurs secondary to the collapse of the Z-disk. Sarcomere disassembly was concurrent with the dissolution of the nuclear envelope. Inhibitors of several intracellular proteases could not block the disassembly of α-actinin or titin. There was a dramatic increase in both cytosolic (soluble) and sarcomeric α-actinin during mitosis, and cytosolic α-actinin exhibited decreased phosphorylation compared to sarcomeric α-actinin. Inhibition of cyclin-dependent kinase 1 (CDK1) induced the quick reassembly of the sarcomere. Sarcomere dis- and re-assembly in cardiomyocyte mitosis is CDK1-dependent and features dynamic differential post-translational modifications of sarcomeric and cytosolic α-actinin.

  16. Endocytosis of PEGylated nanoparticles accompanied by structural and free energy changes of the grafted polyethylene glycol.

    Science.gov (United States)

    Li, Ying; Kröger, Martin; Liu, Wing Kam

    2014-10-01

    Nanoparticles (NPs) are in use to efficiently deliver drug molecules into diseased cells. The surfaces of NPs are usually grafted with polyethylene glycol (PEG) polymers, during so-called PEGylation, to improve water solubility, avoid aggregation, and prevent opsonization during blood circulation. The interplay between grafting density σp and grafted PEG polymerization degree N makes cellular uptake of PEGylated NPs distinct from that of bare NPs. To understand the role played by grafted PEG polymers, we study the endocytosis of 8 nm sized PEGylated NPs with different σp and N through large scale dissipative particle dynamics (DPD) simulations. The free energy change Fpolymer of grafted PEG polymers, before and after endocytosis, is identified to have an effect which is comparable to, or even larger than, the bending energy of the membrane during endocytosis. Based on self-consistent field theory Fpolymer is found to be dependent on both σp and N. By incorporating Fpolymer, the critical ligand-receptor binding strength for PEGylated NPs to be internalized can be correctly predicted by a simple analytical equation. Without considering Fpolymer, it turns out impossible to predict whether the PEGylated NPs will be delivered into the diseased cells. These simulation results and theoretical analysis not only provide new insights into the endocytosis process of PEGylated NPs, but also shed light on the underlying physical mechanisms, which can be utilized for designing efficient PEGylated NP-based therapeutic carriers with improved cellular targeting and uptake.

  17. Impaired Corpus Cavernosum Relaxation Is Accompanied by Increased Oxidative Stress and Up-Regulation of the Rho-Kinase Pathway in Diabetic (Db/Db) Mice.

    Science.gov (United States)

    Priviero, Fernanda B M; Toque, Haroldo A F; Nunes, Kenia Pedrosa; Priolli, Denise G; Teixeira, Cleber E; Webb, R Clinton

    2016-01-01

    Basal release of nitric oxide from endothelial cells modulates contractile activity in the corpus cavernosum via inhibition of the RhoA/Rho-kinase signaling pathway. We aimed to investigate nitric oxide bioavailability, oxidative stress and the Rho-kinase pathway in the relaxation of the corpus cavernosum of an obese and diabetic model of mice (db/db mice). We hypothesized that in db/db mice impaired relaxation induced by Rho-kinase inhibitor is accompanied by diminished NO bioavailability, increased oxidative stress and upregulation of the RhoA/Rho-kinase signalling pathway. Cavernosal strips from male lean and non-diabetic db/+ and db/db mice were mounted in myographs and isometric force in response to Rho-kinase inhibitor Y-27632 was recorded. Enzyme activity and protein expression of oxidative stress markers and key molecules of the RhoA/Rho-kinase pathway were analyzed. The Rho-kinase inhibitor Y-27632 concentration-dependently caused corpus cavernosum relaxation and inhibited cavernosal contractions. Nonetheless, a rightward shift in the curves obtained in corpus cavernosum of db/db mice was observed. Compared to db/+, this strain presented increased active RhoA, higher MYPT-1 phosphorylation stimulated by phenylephrine, and increased expression of ROKα and Rho-GEFs. Further, we observed normal expression of endothelial and neuronal NOS in corpus cavernosum of db/db mice. However, nitrate/nitrate (NOx) levels were diminished, suggesting decreased NO bioavailability. We measured the oxidant status and observed increased lipid peroxidation, with decreased SOD activity and expression. In conclusion, our data demonstrate that in db/db mice, upregulation of the RhoA/Rho-kinase signalling pathway was accompanied by decreased NO bioavailability and increased oxidative stress contributing to impaired relaxation of the corpus cavermosum of db/db mice.

  18. Impaired Corpus Cavernosum Relaxation Is Accompanied by Increased Oxidative Stress and Up-Regulation of the Rho-Kinase Pathway in Diabetic (Db/Db Mice.

    Directory of Open Access Journals (Sweden)

    Fernanda B M Priviero

    Full Text Available Basal release of nitric oxide from endothelial cells modulates contractile activity in the corpus cavernosum via inhibition of the RhoA/Rho-kinase signaling pathway. We aimed to investigate nitric oxide bioavailability, oxidative stress and the Rho-kinase pathway in the relaxation of the corpus cavernosum of an obese and diabetic model of mice (db/db mice. We hypothesized that in db/db mice impaired relaxation induced by Rho-kinase inhibitor is accompanied by diminished NO bioavailability, increased oxidative stress and upregulation of the RhoA/Rho-kinase signalling pathway. Cavernosal strips from male lean and non-diabetic db/+ and db/db mice were mounted in myographs and isometric force in response to Rho-kinase inhibitor Y-27632 was recorded. Enzyme activity and protein expression of oxidative stress markers and key molecules of the RhoA/Rho-kinase pathway were analyzed. The Rho-kinase inhibitor Y-27632 concentration-dependently caused corpus cavernosum relaxation and inhibited cavernosal contractions. Nonetheless, a rightward shift in the curves obtained in corpus cavernosum of db/db mice was observed. Compared to db/+, this strain presented increased active RhoA, higher MYPT-1 phosphorylation stimulated by phenylephrine, and increased expression of ROKα and Rho-GEFs. Further, we observed normal expression of endothelial and neuronal NOS in corpus cavernosum of db/db mice. However, nitrate/nitrate (NOx levels were diminished, suggesting decreased NO bioavailability. We measured the oxidant status and observed increased lipid peroxidation, with decreased SOD activity and expression. In conclusion, our data demonstrate that in db/db mice, upregulation of the RhoA/Rho-kinase signalling pathway was accompanied by decreased NO bioavailability and increased oxidative stress contributing to impaired relaxation of the corpus cavermosum of db/db mice.

  19. Relationship between lipid profile, cardiac hemodynamics status and autoimmune activation markers in patients with ischemic heart disease, accompanied with hypothyroidism

    Directory of Open Access Journals (Sweden)

    N. S. Mykhailovska

    2015-11-01

    Full Text Available Presence of hypothyroidism in patients with coronary heart disease contribute to its progression. Aim. Study purpose was exploring the features of changes in lipid profile, cardiac hemodynamics status, autoimmune activation markers and their relationships in patients with coronary heart disease, accompanied with hypothyroidism. Methods and results. 64 patients were examined. The levels of thyroid-pituitary hormones, concentration of tumor necrosis factor-α, C-reactive protein, endothelin-1, an inhibitor of plasminogen activator-1, neopterin were examined by ELISA. Structural and functional heart features were studied with doppler echocardiography. It was found that patients with coronary heart disease, accompanied with hypothyroidism, had more expressed lipid disorders, heart restructuring such as increased end - diastolic volume, end - diastolic pressure, myocardial mass and stiffness index on the background of immunoinflammatory activation and endothelial dysfunction. Conclusions. It was found correlation between markers of endothelial dysfunction immunoinflammatory activation and lipid profile, heart structural and functional state.

  20. WEATHER AND CIRCULATION TYPES ACCOMPANYING THERMAL AND HUMIDITY CONDITIONS UNFAVOURABLE TO THE HUMAN HEALTH IN SUMMER IN KRAKOW (POLAND

    Directory of Open Access Journals (Sweden)

    D. CIARANEK

    2013-03-01

    Full Text Available The paper presents the results of a study analysing the frequency of occurrence and patterns of change in the human perception of temperature in relation to types of weather and circulation in Krakow. The Humidex index used for the purpose was determined at three measurement times (6, 12, 18 UTC with data spanning the period 1961-2012. The frequency of occurrence of all types of discomfort situation was found to be on the increase. Days with some discomfort occurred most frequently during non-advection situations, (especially in the centre of a meteorological high or anticyclonic wedge, accompanied by transformed polar maritime air or continental polar air. The weather varied greatly on such days, from cloudy to sunny and with or without precipitation. Days with high discomfort levels were associated with the advection of tropical air accompanied typically by very hot, sweltering weather, and by dry, very sunny weather.

  1. Bioeffect of lipohemia rabbits irradiated in oral mucosa with 650-nm diode-laser-accompanied oxygen inspiration and clinical application

    Science.gov (United States)

    Yang, Fu-Shou; Tang, Jin-Xian; Liu, Cheng; Yang, Xi-Cheng; Pang, Hi-Xiu

    1998-11-01

    The study on irradiating in oral mucosa of rabbits with 650 nm diode laser and clinical application has been reported in this paper. The result of animal experiment showed: the obvious decrease of cholesterin and triglyceride has been found among those highly lipohemia rabbits in the experiments of 650nm diode laser irradiating accompanying with oxygen, as well as the parameters of hemorheology obviously being improved, as compared with highly lipohemia rabbits un-irradiating, the statistical analysis showing P renal interstitial in the group of rabbits which are irradiated with laser and accompanying with oxygen inspiration, and even the perfectly recovered tissue in some rabbits has been seen. This experimental result is significantly for clinical application. The results of clinic application showed, that the patients employed this method which treatment cerebral infarction, lipohemia, the total effective ratio achieved 91.7 percent, perfect effect 30.6 percent.

  2. New dance piano accompaniment teaching practice%舞蹈钢琴伴奏的教学实践新探

    Institute of Scientific and Technical Information of China (English)

    项征

    2015-01-01

    舞蹈钢琴伴奏教学不仅是一门重要的艺术类学科,更是一门横跨舞蹈和音乐两大艺术的学科,以舞蹈教学为主,以音乐伴奏为辅,通过音乐的衬托来让学生在一定的意境下学习相关知识,对培养舞蹈艺术人才有着极大的作用.%Dance piano accompaniment teaching is not only a important arts discipline, but also an art subject across dance and music, give priority to with the dance teaching, with music accompaniment is complementary, foil through music to let students study related knowledge, under a certain artistic conception has a great role on training dance art talents.

  3. Relationship between lipid profile, cardiac hemodynamics status and autoimmune activation markers in patients with ischemic heart disease, accompanied with hypothyroidism

    Directory of Open Access Journals (Sweden)

    N. S. Mykhailovska

    2015-12-01

    Full Text Available Presence of hypothyroidism in patients with coronary heart disease contribute to its progression. Aim. Study purpose was exploring the features of changes in lipid profile, cardiac hemodynamics status, autoimmune activation markers and their relationships in patients with coronary heart disease, accompanied with hypothyroidism. Methods and results. 64 patients were examined. The levels of thyroid-pituitary hormones, concentration of tumor necrosis factor-α, C-reactive protein, endothelin-1, an inhibitor of plasminogen activator-1, neopterin were examined by ELISA. Structural and functional heart features were studied with doppler echocardiography. It was found that patients with coronary heart disease, accompanied with hypothyroidism, had more expressed lipid disorders, heart restructuring such as increased end - diastolic volume, end - diastolic pressure, myocardial mass and stiffness index on the background of immunoinflammatory activation and endothelial dysfunction. Conclusions. It was found correlation between markers of endothelial dysfunction immunoinflammatory activation and lipid profile, heart structural and functional state.

  4. Primary Sj(o)gren's Syndrome Accompanied by Intestinal Obstruction: a Case Report and Literature Review

    Institute of Scientific and Technical Information of China (English)

    Jie-ting Jia; Hua Wei; Hui Li

    2012-01-01

    SJ(O)GREN'S syndrome (SS) is a chronic inflammatory autoimmune disease characterized by the infiltration of lymphocytes and plasma cells in exocrine glands,especially salivary and lacrimal gland interstitium.The clinical manifestations of SS are complex.When the digestive system is involved,the patient always presents with liver damage,atrophic gastritis,and chronic diarrhea.Intestinal obstruction is rare and the specific pathogenesis remains unclear.1 Currently,there is no clear treatment guidelines for SS with intestinal obstruction.The general measures include gastrointestinal decompression,spasmolysis,and other symptomatic treatments and the use of glucocorticoid to control the progressing of disease.Here we describe a case of primary SS with intestinal obstruction who was treated with conservative treatment.

  5. A Patient With Primary Biliary Cirrhosis Accompanied by Wilson’s Disease

    Directory of Open Access Journals (Sweden)

    Zhao

    2016-02-01

    Full Text Available Introduction Both primary biliary cirrhosis (PBC and Wilson’s disease (WD can cause copper retention in the liver, which is an important factor for liver cellular damage. Copper chelation may preserve liver cell function. It is challenging to distinguish WD from copper accumulation in patients with PBC. There have been few case reports of PBC co-occurrence with WD. Case Presentation Here we report a case of PBC with WD in a 55-year-old Chinese male. In addition to the typical pathological characteristics of PBC and a large number of copper depositions in the liver, the patient showed WD ATP7B gene mutations. Conclusions Co-occurrence of PBC with WD is rare, which can cause diffusely intrahepatic copper deposition. Early liver biopsy and genetic testing are necessary for the diagnosis. The combination of ursodeoxycholic acid with zinc and sodium dimercaptopropane sulfonate is effective.

  6. Autoimmune thrombocytopenia in response to splenectomy in cirrhotic patients with accompanying hepatitis C

    Institute of Scientific and Technical Information of China (English)

    Tetsuro Sekiguchi; Takeaki Nagamine; Hitoshi Takagi; Masatomo Mori

    2006-01-01

    AIM: To estimate the contribution of autoimmune thrombocytopenia to hepatitis C virus-related liver cirrhosis (type C cirrhosis), we evaluated the influence of splenectomy upon platelet-associated immunoglobulin G (PAIgG) levels and platelet numbers.METHODS: PAIgG titers and immune markers were determined in 24 type C cirrhotic patients with an intact spleen, 17 type C cirrhotic patients submitted to splenectomy, and 21 non-C cirrhosis with an intact spleen.RESULTS: Thrombocytopenia (PLT<15×104/μL) in type C cirrhosis was diagnosed in all patients with an intact spleen, 8 patients submitted to splenectomy, and in 19 non-C cirrhosis with intact spleen. Elevated titers of PAIgG at more than 25.0 ng/107 cells were detected in all cirrhotic patients except for one splenectomized patient.PAIgG titers (ng/107 cells) were significantly higher in the type C cirrhosis with an intact spleen (247.9 ± 197.0)compared with the splenectomized patients (125.6±87.8)or non-C cirrhosis (152.4± 127.4). PAIgG titers were negatively correlated with platelet counts in type C cirrhotic patients with an intact spleen. In comparison with the type C cirrhosis with an intact spleen, the splenectomized patients had a reduced CD4/CD8 ratio and serum neopterin levels. The spleen index (cm2) was negatively correlated with platelet counts in the non-C cirrhosis, but not in the type C cirrhosis.CONCLUSION: Our data indicate that the autoimmune mechanism plays an important role in thrombocytosis complicated by HCV-positive cirrhosis. In addition,splenectomy may impair T ceils function through, at least in part, a reduction of CD4/CD8 ratio, consequently suppressing PAIgG production.

  7. Comparative effectiveness of second-generation antidepressants for accompanying anxiety, insomnia, and pain in depressed patients: a systematic review.

    Science.gov (United States)

    Thaler, Kylie J; Morgan, Laura C; Van Noord, Megan; Gaynes, Bradley N; Hansen, Richard A; Lux, Linda J; Krebs, Erin E; Lohr, Kathleen N; Gartlehner, Gerald

    2012-06-01

    Patients with major depressive disorder (MDD) often suffer from accompanying symptoms that influence the choice of pharmacotherapy with second-generation antidepressants (SGAs). We conducted a systematic review to determine the comparative effectiveness of citalopram, escitalopram, fluoxetine, fluvoxamine, paroxetine, sertraline, desvenlafaxine, duloxetine, venlafaxine, bupropion, mirtazapine, nefazodone, and trazodone, for accompanying anxiety, insomnia, and pain in patients with MDD. We conducted searches in multiple databases including MEDLINE®, Embase, the Cochrane Library, International Pharmaceutical Abstracts, and PsycINFO, from 1980 through August 2011 and reviewed reference lists of pertinent articles. We dually reviewed abstracts, full-text articles, and abstracted data. We included randomized, head-to-head trials of SGAs of at least 6 weeks' duration. We grouped SGAs into three classes for the analysis: selective serotonin reuptake inhibitors (SSRIs), serotonin norepinephrine reuptake inhibitors, and others. We graded the strength of the evidence as high, moderate, low, or very low based on the Grading of Recommendations Assessment, Development and Evaluation (GRADE) Working Group (GRADE) approach. We located 19 head-to-head trials in total: 11 on anxiety, six on insomnia, and four on pain. For the majority of comparisons, the strength of the evidence was moderate or low: evidence is weakened by inconsistency and imprecision. For treating anxiety, insomnia, and pain moderate evidence suggests that the SSRIs do not differ. Evidence guiding the selection of an SGA based on accompanying symptoms of depression is limited. Very few trials were designed and adequately powered to answer questions about accompanying symptoms; analyses were generally of subgroups in larger MDD trials. © 2012 Wiley Periodicals, Inc.

  8. Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria.

    OpenAIRE

    Setty, O H; Shrager, R I; Bunow, B; Reynafarje, B; Lehninger, A L; Hendler, R W

    1986-01-01

    The problem of obtaining very early ratios for the H+/O stoichiometry accompanying succinate oxidation by rat liver mitochondria was attacked using new techniques for direct measurement rather than extrapolations based on data obtained after mixing and the recovery of the electrode from initial injection of O2. Respiration was quickly initiated in a thoroughly mixed O2-containing suspension of mitochondria under a CO atmosphere by photolysis of the CO-cytochrome c oxidase complex-. Fast respo...

  9. Deformation effects in the alpha accompanied cold ternary fission of even-even 244-260Cf isotopes

    Science.gov (United States)

    Santhosh, K. P.; Krishnan, Sreejith

    2016-04-01

    Within the unified ternary fission model (UTFM), the alpha accompanied ternary fission of even-even 244-260Cf isotopes has been studied by taking the interacting barrier as the sum of Coulomb and proximity potential. For the alpha accompanied ternary fission of the 244Cf isotope, the highest yield is obtained for the fragment combination 108Ru + 4He + 132Te, which contains the near doubly magic nucleus 132Te ( N = 80, Z = 52). In the case of 246Cf and 248Cf isotopes, the highest yield is obtained for the fragment combinations with the near doubly magic nucleus 134Te ( N = 82, Z = 52) as the heaviest fragment. The highest yield obtained for 250Cf, 252Cf, 254Cf, 256Cf, 258Cf and 260Cf isotopes is for the fragment combination with the doubly magic nucleus 132Sn ( N = 82), Z = 50 as the heaviest fragment. We have included the effect of deformation and orientation of fragments and this has revealed that in addition to the closed shell effect, ground-state deformation also plays an important role in the calculation of the relative yield of favorable fragment combinations. The computed isotopic yields for the alpha accompanied ternary fission of the 252Cf isotope are found to be in agreement with the experimental data. The emission probability and kinetic energy of the long-range alpha particle is calculated for the various isotopes of Cf and are found to be in good agreement with the experimental data.

  10. {\\alpha}-accompanied cold ternary fission of $^{238-244}$Pu isotopes in equatorial and collinear configuration

    CERN Document Server

    Santhosh, K P; Priyanka, B

    2015-01-01

    The cold ternary fission of $^{238}$Pu, $^{240}$Pu, $^{242}$Pu and $^{244}$Pu isotopes, with $^{4}$He as light charged particle, in equatorial and collinear configuration has been studied within the Unified ternary fission model (UTFM). The fragment combination $^{100}$Zr+$^{4}$He+$^{134}$Te possessing the near doubly magic nuclei $^{134}$Te (N=82, Z=52) gives the highest yield in the alpha accompanied ternary fission of $^{238}$Pu. For the alpha accompanied ternary fission of $^{240}$Pu, $^{242}$Pu and $^{244}$Pu isotopes, the highest yield was found for the fragment combination with doubly magic nuclei $^{132}$Sn (N=82, Z=50) as the heavier fragment. The deformation and orientation of fragments have also been taken into account for the alpha accompanied ternary fission of $^{238-244}$Pu isotopes, and it has been found that in addition to closed shell effect, ground state deformation also plays an important role in determining the isotopic yield in the ternary fission process. The emission probability and ki...

  11. Efficacy of application technology of managing physical exercise by the musical accompaniment to reduce school anxiety first form pupils

    Directory of Open Access Journals (Sweden)

    Smirnova Y.V.

    2014-01-01

    Full Text Available Purpose : to study the need to improve the mental state of first-graders and the possibility of achieving this goal, the lesson of physical culture at the expense of proper use of musical accompaniment exercise. Material : participated in the experiment experimental, control and background group (only 55 students of first class. Results : argues that music has a regulating effect on mental and physiological state of a person. It is noted that the combined effects of exercise and music on the body and psyche of a child reduces total anxiety in school. Also reduces the fear in a meaningful situation knowledge test. Found that the effect of exercise without music and with various embodiments use musical accompaniment in the lesson to address interpersonal relations student and the teacher has no significant difference. Conclusions : as a result of the pedagogical experiment to identify significantly positive, confirming the effectiveness of the technology of managing physical exercise by the musical accompaniment for the performance of most indicators of school anxiety.

  12. The Contribution of Conceptual Change Texts Accompanied by Concept Mapping to Eleventh-Grade Students Understanding of Cellular Respiration Concepts

    Science.gov (United States)

    Al Khawaldeh, Salem A.; Al Olaimat, Ali M.

    2010-04-01

    The present study conducted to investigate the contribution of conceptual change texts, accompanied by concept mapping instruction to eleventh-grade students' understanding of cellular respiration concepts, and their retention of this understanding. Cellular respiration concepts test was developed as a result of examination of related literature and interviews with teachers regarding their observations of students' difficulties. The test was administrated as pre-test, post-test, and delayed post-test to a total of 70 eleventh-grade students in two classes of the same high school in an urban area, taught by the same teacher. The experimental group was a class of 34 students who received conceptual change texts accompanied by concept mapping instruction. A class of 36 students comprised the control group who received traditional instruction. Besides treatment, previous understanding and logical thinking ability were other independent variables involved in this study. The results showed that logical thinking, treatment, previous understanding of cellular respiration concepts each made a statistically significant contribution to the variation in students' understanding of cellular respiration concepts. The result also showed that conceptual change texts accompanied by concept mapping instruction was significantly better than traditional instruction in retention of this understanding.

  13. Effects of Progressive Muscle Relaxation Exercises Accompanied by Music on Low Back Pain and Quality of Life During Pregnancy.

    Science.gov (United States)

    Akmeşe, Zehra Baykal; Oran, Nazan Tuna

    2014-01-01

    Back pain is commonly experienced by pregnant women. Evidence suggests that progressive muscle relaxation (PMR) therapy, a complementary therapy widely used by pregnant women, may improve the physical and psychological outcomes of pregnancy. The aim of this study was to investigate the effects of PMR training accompanied by music on perceived pain and quality of life (QOL) in pregnant women with low back pain (LBP). This was a prospective randomized controlled trial. The study was designed to examine the effects of PMR accompanied by music on pregnant women with LBP. In total, 66 pregnant women were assigned randomly to a PMR group or a control group (33 women in each). A personal information form was used as a data collection tool; a visual analog scale was used for measuring pain; and the Short Form-36 was used to evaluate QOL. The control and intervention groups were comparable at baseline. Significant differences were observed between the 2 groups after 4 and 8 weeks of intervention. The intervention group showed significant improvement in all QOL subscales after the intervention. The intervention group, but not the control group, showed significant improvement in perceived pain after the intervention. The intervention group experienced a greater decrease in perceived pain and improved QOL than the control group. Our findings show that PMR accompanied by music may be an effective therapy for improving pain and QOL in pregnant women with LBP. Large randomized studies are recommended to confirm these results. © 2014 by the American College of Nurse‐Midwives.

  14. Cells

    Directory of Open Access Journals (Sweden)

    Zhao-Hui Jin

    2012-11-01

    Full Text Available As cancer stem cells (CSCs are postulated to play critical roles in cancer development, including metastasis and recurrence, CSC imaging would provide valuable information for cancer treatment and lead to CSC-targeted therapy. To assess the possibility of in vivo CSC targeting, we conducted basic studies on radioimmunotargeting of cancer cells positive for CD133, a CSC marker recognized in various cancers. Antibodies against CD133 were labeled with 125I, and their in vitro cell binding properties were tested. Using the same isotype IgG as a control, in vivo biodistribution of the labeled antibody retaining immunoreactivity was examined in mice bearing an HCT116 xenograft in which a population of the cancer cells expressed CD133. Intratumoral distribution of the labeled antibody was examined and compared to the CD133 expression pattern. The 125I-labeled anti-CD133 antibody showed a modest but significantly higher accumulation in the HCT116 xenograft compared to the control IgG. The intratumoral distribution of the labeled antibody mostly overlapped with the CD133 expression, whereas the control IgG was found in the area close to the necrotic tumor center. Our results indicate that noninvasive in vivo targeting of CSCs could be possible with radiolabeled antibodies against cell membrane markers.

  15. Clinical investigation of 41 patients with ischemic colitis accompanied by ulcer

    Institute of Scientific and Technical Information of China (English)

    Satohiro Matsumoto; Kenichiro Tsuji; Satoshi Shirahama

    2007-01-01

    AIM: To investigate the relationship among the presence of ulcer lesions, underlying disease, and clinical course in patients with ischemic colitis.METHODS: The subjects were 41 patients (10 male and 31 female; mean age 70 years) with ischemic colitis who were admitted to and received treatment in our hospital from 2000 to 2006. We compared their characteristics and analyzed the mean lengths of admission and fasting for 9 patients with ulcer lesions (ulcer group) and 32 without (non-ulcer group).RESULTS: The groups with presence and absence of ulcer differed significantly only in white blood cell (WBC)count. Lengths of fasting and admission were 7.9 d and 17.9 d for the ulcer group and 4.4 d and 10.7 d for the non-ulcer group, respectively, and significantly longer in the ulcer group (P = 0.0057 and 0.0001). There was no correlation between presence of ulcer and presence of underlying diseases.CONCLUSION: Lengths of fasting and admission were significantly longer in patients with ischemic colitis with ulcer than for those without ulcer.

  16. Massive Amplification at an Unselected Locus Accompanies Complex Chromosomal Rearrangements in Yeast

    Directory of Open Access Journals (Sweden)

    Agnès Thierry

    2016-05-01

    Full Text Available Gene amplification has been observed in different organisms in response to environmental constraints, such as limited nutrients or exposure to a variety of toxic compounds, conferring them with specific phenotypic adaptations via increased expression levels. However, the presence of multiple gene copies in natural genomes has generally not been found in the absence of specific functional selection. Here, we show that the massive amplification of a chromosomal locus (up to 880 copies per cell occurs in the absence of any direct selection, and is associated with low-order amplifications of flanking segments in complex chromosomal alterations. These results were obtained from mutants with restored phenotypes that spontaneously appeared from genetically engineered strains of the yeast Saccharomyces cerevisiae suffering from severe fitness reduction. Grossly extended chromosomes (macrotene were formed, with complex structural alterations but sufficient stability to propagate unchanged over successive generations. Their detailed molecular analysis, including complete genome sequencing, identification of sequence breakpoints, and comparisons between mutants, revealed novel mechanisms causing their formation, whose combined action underlies the astonishing dynamics of eukaryotic chromosomes and their consequences.

  17. Multiple carcinoids in the duodenum, pancreas and stomach accompanied with type A gastritis: A case report

    Institute of Scientific and Technical Information of China (English)

    Takeo Bamba; Shin-ichi Kosugi; Tatsuo Kanda; Toshihiro Tsubono; Yasuo Sakai; Nobuyuki Musha; Noriko Ishihara; Katsuyoshi Hatakeyama

    2007-01-01

    We report a case of multiple duodenal, pancreatic, and gastric carcinoids. A 67-year old woman was admitted to our hospital for treatment of a duodenal carcinoid.Laboratory tests revealed that the patient was associated with macrocytic anemia and hypergastrinemia, and type A gastritis was shown by gastrofiberscopy.During surgery, another tumor was incidentally found in the head of the pancreas. The tumors in the duodenum and pancreas were completely excised by pancreatoduodenectomy and immunohistologically diagnosed as gastrin-and serotonin-producing carcinoids,respectively. Pathological examination revealed that in addition to the grossly found carcinoids, there were subclinical carcinoids, one of which was an endocrine cell micronest, located in the stomach and duodenum.The tumors in the duodenum, pancreas, and stomach showed different characteristics from one another morphologically and immunochemically. Although no definitive evidence has been obtained, some sort of genetic anomaly may have been involved in this case,and hypergastrinemia due to duodenal gastrinoma may induce multiple gastric carcinoids.

  18. Changes in the Transcriptome of Human Astrocytes Accompanying Oxidative Stress-Induced Senescence

    Science.gov (United States)

    Crowe, Elizabeth P.; Tuzer, Ferit; Gregory, Brian D.; Donahue, Greg; Gosai, Sager J.; Cohen, Justin; Leung, Yuk Y.; Yetkin, Emre; Nativio, Raffaella; Wang, Li-San; Sell, Christian; Bonini, Nancy M.; Berger, Shelley L.; Johnson, F. Brad; Torres, Claudio

    2016-01-01

    Aging is a major risk factor for many neurodegenerative disorders. A key feature of aging biology that may underlie these diseases is cellular senescence. Senescent cells accumulate in tissues with age, undergo widespread changes in gene expression, and typically demonstrate altered, pro-inflammatory profiles. Astrocyte senescence has been implicated in neurodegenerative disease, and to better understand senescence-associated changes in astrocytes, we investigated changes in their transcriptome using RNA sequencing. Senescence was induced in human fetal astrocytes by transient oxidative stress. Brain-expressed genes, including those involved in neuronal development and differentiation, were downregulated in senescent astrocytes. Remarkably, several genes indicative of astrocytic responses to injury were also downregulated, including glial fibrillary acidic protein and genes involved in the processing and presentation of antigens by major histocompatibility complex class II proteins, while pro-inflammatory genes were upregulated. Overall, our findings suggest that senescence-related changes in the function of astrocytes may impact the pathogenesis of age-related brain disorders. PMID:27630559

  19. Changes in the Transcriptome of Human Astrocytes Accompanying Oxidative Stress-induced Senescence

    Directory of Open Access Journals (Sweden)

    Elizabeth P. Crowe

    2016-08-01

    Full Text Available Aging is a major risk factor for many neurodegenerative disorders. A key feature of aging biology that may underlie these diseases is cellular senescence. Senescent cells accumulate in tissues with age, undergo widespread changes in gene expression, and typically demonstrate altered, pro-inflammatory profiles. Astrocyte senescence has been implicated in neurodegenerative disease, and to better understand senescence-associated changes in astrocytes, we investigated changes in their transcriptome using RNA sequencing. Senescence was induced in human fetal astrocytes by transient oxidative stress. Brain-expressed genes, including those involved in neuronal development and differentiation, were downregulated in senescent astrocytes. Remarkably, several genes indicative of astrocytic responses to injury were also downregulated, including GFAP and genes involved in the processing and presentation of antigens by major histocompatibility complex class II proteins, while pro-inflammatory genes were upregulated. Overall, our findings suggest that senescence-related changes in the function of astrocytes may impact the pathogenesis of age-related brain disorders.

  20. An increase in pectin methyl esterase activity accompanies dormancy breakage and germination of yellow cedar seeds.

    Science.gov (United States)

    Ren, C; Kermode, A R

    2000-09-01

    Pectin methyl esterase (PME) (EC 3.1.1.11) catalyzes the hydrolysis of methylester groups of cell wall pectins. We investigated the role of this enzyme in dormancy termination and germination of yellow cedar (Chamaecyparis nootkatensis [D. Don] Spach) seeds. PME activity was not detected in dormant seeds of yellow cedar but was induced and gradually increased during moist chilling; high activity coincided with dormancy breakage and germination. PME activity was positively correlated to the degree of dormancy breakage of yellow cedar seeds. The enzyme produced in different seed parts and in seeds at different times during moist chilling, germination, and early post-germinative growth consisted of two isoforms, both basic with isoelectric points of 8.7 and 8.9 and the same molecular mass of 62 kD. The pH optimum for the enzyme was between 7.4 and 8.4. In intact yellow cedar seeds, activities of the two basic isoforms of PME that were induced in embryos and in megagametophytes following dormancy breakage were significantly suppressed by abscisic acid. Gibberellic acid had a stimulatory effect on the activities of these isoforms in embryos and megagametophytes of intact seeds at the germinative stage. We hypothesize that PME plays a role in weakening of the megagametophyte, allowing radicle emergence and the completion of germination.

  1. Chemical -induced apoptotic cell death in tomato cells : involvement of caspase-like proteases

    NARCIS (Netherlands)

    Jong, de A.J.; Hoeberichts, F.A.; Yakimova, E.T.; Maximova, E.; Woltering, E.J.

    2000-01-01

    A new system to study programmed cell death in plants is described. Tomato (Lycopersicon esculentum Mill.) suspension cells were induced to undergo programmed cell death by treatment with known inducers of apoptosis in mammalian cells. This chemical-induced cell death was accompanied by the characte

  2. Deformation effects in the alpha accompanied cold ternary fission of even-even {sup 244-260}Cf isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Santhosh, K.P.; Krishnan, Sreejith [Kannur University, School of Pure and Applied Physics, Payyanur, Kerala (India)

    2016-04-15

    Within the unified ternary fission model (UTFM), the alpha accompanied ternary fission of even-even {sup 244-260}Cf isotopes has been studied by taking the interacting barrier as the sum of Coulomb and proximity potential. For the alpha accompanied ternary fission of the {sup 244}Cf isotope, the highest yield is obtained for the fragment combination {sup 108}Ru + {sup 4}He + {sup 132}Te, which contains the near doubly magic nucleus {sup 132}Te (N = 80, Z = 52). In the case of {sup 246}Cf and {sup 248}Cf isotopes, the highest yield is obtained for the fragment combinations with the near doubly magic nucleus {sup 134}Te (N = 82, Z = 52) as the heaviest fragment. The highest yield obtained for {sup 250}Cf, {sup 252}Cf, {sup 254}Cf, {sup 256}Cf, {sup 258}Cf and {sup 260}Cf isotopes is for the fragment combination with the doubly magic nucleus {sup 132}Sn (N = 82), Z = 50 as the heaviest fragment. We have included the effect of deformation and orientation of fragments and this has revealed that in addition to the closed shell effect, ground-state deformation also plays an important role in the calculation of the relative yield of favorable fragment combinations. The computed isotopic yields for the alpha accompanied ternary fission of the {sup 252}Cf isotope are found to be in agreement with the experimental data. The emission probability and kinetic energy of the long-range alpha particle is calculated for the various isotopes of Cf and are found to be in good agreement with the experimental data. (orig.)

  3. Analysis of 300 cases of doula accompany delivery%导乐陪伴分娩300例分析

    Institute of Scientific and Technical Information of China (English)

    陈志兵; 蒋美玲; 张卫文

    2012-01-01

    Objective To investigate the impact on improving the quality of obstetric doula accompany delivery. Methods 600 pregnant women were randomly divided into 2 groups (300 cases) : Doula group deliveries throughout a work of serious and responsible,midwives have solid medical theory and extensive clinical experience implementing one - to - one companionship by a midwife; The control group was dealed with the conventional production process handling. Results Doula group vaginal delivery rate was higher than that of control group,and postpartumhemorrhage and neonatal asphyxia were significantly lower than the control group,the differences were statistically significant. Conclusion Doula accompany delivery is a humane medical service mode. Doula accompanied delivery childbirth course allows mothers to experience a healthy and pleasant,and make mother and child safety,health,and it is worthy of promotion.%目的 探讨导乐陪伴分娩对提高产科质量的影响.方法 将600例孕妇随机分为2组(各300例):导乐组分娩全程由一位工作认真负责、具有扎实的医学理论及丰富临床经验的助产士实施一对一陪伴;对照组按传统的模式进行常规产程处理.结果 导乐组阴道分娩率高于对照组,产后出血及新生儿窒息均明显低于对照组,差异均有统计学意义.结论 导乐陪伴分娩是一种人性化医学服务模式,可让母亲们经历健康而愉快的分娩历程,使母婴更加安全、健康,值得推广.

  4. 钢琴即兴伴奏课的教学改革探讨%Impromptu piano accompaniment teaching reform

    Institute of Scientific and Technical Information of China (English)

    李娜

    2015-01-01

    钢琴即兴伴奏是一门综合性课程,集音乐审美能力、和声理论知识、钢琴弹奏技术于一体,并且实践和理论的结核性较强,是相关专业学生的必须科目之一。然而受多因素的制约和影响,使得当下的教学实际成效并不乐观,因此对教学进行全方位改革具有重要性和必要性。下面本文将介绍钢琴即兴伴奏教学的目标与内容,并分析当下的教学现状,并将有效的改革对策提出,以此来推进钢琴即兴伴奏课的开展。%Piano impromptu accompaniment is a comprehensive course, music aesthetic ability, harmony theory knowledge, piano technology at an organic whole, and the practice and theory of n/med tuberculosis sex is stronger, is one of the relevant professional students must subject. However, restricted and influenced by many factors, the teaching real results of the present is not optimistic, so the comprehensive reform of the teaching has the importance and necessity. This article will introduce below impromptu piano accompaniment teaching goal and content, and analyze the status quo of teaching, and effective reform countermeasures, so as to promote piano improvisational accompaniment lesson.

  5. Myocardial infarction worsens glomerular injury and microalbuminuria in rats with pre-existing renal impairment accompanied by the activation of ER stress and inflammation.

    Science.gov (United States)

    Dong, Zhifeng; Wu, Penglong; Li, Yongguang; Shen, Yuan; Xin, Ping; Li, Shuai; Wang, Zhihua; Dai, Xiaoyan; Zhu, Wei; Wei, Meng

    2014-12-01

    Deterioration of renal function occurs after chronic heart failure in approximately one-third of patients, particularly in those with pre-existing renal impairment such as diabetic nephropathy. Impaired renal function in these patients is always associated with a worse prognosis. However, the mechanisms underlying such deterioration of renal function are still largely unknown. In three separate protocols, we compared 1) sham operation (Ctr, n = 10) with surgically induced myocardial infarction (MI, n = 10); 2) unilateral nephrectomy (UNX, n = 10) with UNX + MI (n = 10); and 3) STZ-induced type 1 diabetes (DB, n = 10) with DB + MI (n = 10). The differences between combined injury models (UNX + MI, DB + MI) and simple MI were also examined. Renal remodeling, function, ER stress (CHOP and GRP78) and inflammation (infiltration of inflammatory cells, NF-κB p65) were evaluated 12 weeks after MI. In common SD rats, MI activated less glomerular ER stress and inflammation, resulting in a minor change of glomerular remodeling and microalbuminuria. However, MI significantly increased the glomerular expression of GRP78 and CHOP in UNX and DB rats. In addition, it also promoted the infiltration of CD4+ T cells, particularly inflammatory cytokine (IFN-γ, IL-17, IL-4)-producing CD4+ T cells, and the expression of NF-κB p65 in the glomeruli. By contrast, significant glomerular fibrosis, glomerulosclerosis, podocyte injury and microalbuminuria were found in rats with UNX + MI and DB + MI. MI significantly increased chronic glomerular injury and microalbuminuria at 12 weeks in rats with pre-existing renal impairment, i.e., UNX and DB, but not common SD rats. These changes were accompanied by increased glomerular ER stress and immune-associated inflammation.

  6. 41 CFR 302-4.204 - If my spouse does not accompany me but travels unaccompanied at a different time, what per diem...

    Science.gov (United States)

    2010-07-01

    ... accompany me but travels unaccompanied at a different time, what per diem rate will he/she receive? 302-4.204 Section 302-4.204 Public Contracts and Property Management Federal Travel Regulation System... my spouse does not accompany me but travels unaccompanied at a different time, what per diem rate...

  7. 30 CFR 250.220 - If I propose activities in the Alaska OCS Region, what planning information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... activities in the Alaska OCS Region, what planning information must accompany the EP? If you propose exploration activities in the Alaska OCS Region, the following planning information must accompany your EP: (a... 30 Mineral Resources 2 2010-07-01 2010-07-01 false If I propose activities in the Alaska...

  8. 30 CFR 250.251 - If I propose activities in the Alaska OCS Region, what planning information must accompany the DPP?

    Science.gov (United States)

    2010-07-01

    ... activities in the Alaska OCS Region, the following planning information must accompany your DPP: (a... 30 Mineral Resources 2 2010-07-01 2010-07-01 false If I propose activities in the Alaska OCS Region, what planning information must accompany the DPP? 250.251 Section 250.251 Mineral...

  9. Macroscopic yielding in jammed solids is accompanied by a nonequilibrium first-order transition in particle trajectories

    Science.gov (United States)

    Kawasaki, Takeshi; Berthier, Ludovic

    2016-08-01

    We use computer simulations to analyze the yielding transition during large-amplitude oscillatory shear of a simple model for soft jammed solids. Simultaneous analysis of global mechanical response and particle-scale motion demonstrates that macroscopic yielding, revealed by a smooth crossover in mechanical properties, is accompanied by a sudden change in the particle dynamics, which evolves from nondiffusive motion to irreversible diffusion as the amplitude of the shear is increased. We provide numerical evidence that this sharp change corresponds to a nonequilibrium first-order dynamic phase transition, thus establishing the existence of a well-defined microscopic dynamic signature of the yielding transition in amorphous materials in oscillatory shear.

  10. Double K-shell ionization accompanying internal conversion of the 0.662-MeV transition in 137Bam

    Science.gov (United States)

    Nagy, H. J.; Brady, K. E.; Schupp, G.

    1988-12-01

    Double ionization of the atomic K shell accompanying K-shell internal conversion of the 0.662-MeV transition of 137Bam has been studied by recording coincidences between Kα hypersatellite x rays and Kα satellite x rays emitted when the double vacancies are filled. The probability per K-shell internal conversion that a double vacancy is formed, PKK(IC), was found to be (10.0+/-0.9)×10-5 which is in general agreement with less precise earlier experiments, but is a factor of 2.7+/-0.3 larger than the relativistic, one-step theory of Mukoyama and Shimizu.

  11. [Retroperitoneal dedifferentiated liposarcoma extending into the iliocostal muscle and the quadratus lumborum muscle accompanied with bone formation: case report].

    Science.gov (United States)

    Matsuura, H; Sakurai, M; Arima, K

    2001-12-01

    A 72-year-old man with back pain on the left side was admitted. Imaging analysis revealed a retroperitoneal mass and a mass in the left iliocostal muscle and the left quadratus lumborum muscle. The two masses could not be resected en bloc, and were resected separately. The clinicopathological findings of these tumors revealed dedifferentiated liposarcoma. The primary dedifferentiated liposarcoma appeared to have originated from the retroperitoneal space extending into the iliocostal muscle and the quadratus lumborum muscle. Then the mass was thought to have formed accompanied with osteogenesis.

  12. More than Meets the Eye: Functionally Salient Changes in Internal Bone Architecture Accompany Divergence in Cichlid Feeding Mode

    Directory of Open Access Journals (Sweden)

    R. Craig Albertson

    2012-01-01

    Full Text Available African cichlids have undergone extensive and repeated adaptive radiations in foraging habitat. While the external morphology of the cichlid craniofacial skeleton has been studied extensively, biomechanically relevant changes to internal bone architecture have been largely overlooked. Here we explore two fundamental questions: (1 Do changes in the internal architecture of bone accompany shifts in foraging mode? (2 What is the genetic basis for this trait? We focus on the maxilla, which is an integral part of the feeding apparatus and an element that should be subjected to significant bending forces during biting. Analyses of μCT scans revealed clear differences between the maxilla of two species that employ alternative foraging strategies (i.e., biting versus suction feeding. Hybrids between the two species exhibit maxillary geometries that closely resemble those of the suction feeding species, consistent with a dominant mode of inheritance. This was supported by the results of a genetic mapping experiment, where suction feeding alleles were dominant to biting alleles at two loci that affect bone architecture. Overall, these data suggest that the internal structure of the cichlid maxilla has a tractable genetic basis and that discrete shifts in this trait have accompanied the evolution of alternate feeding modes.

  13. Deformation effects in the alpha accompanied cold ternary fission of even-even $^{244-260}$Cf isotopes

    CERN Document Server

    Santhosh, K P

    2016-01-01

    Within the Unified ternary fission model (UTFM), the alpha accompanied ternary fission of even-even $^{244-260}$Cf isotopes has been studied by taking the interacting barrier as the sum of Coulomb and proximity potential. For the alpha accompanied ternary fission of 244^Cf isotope, the highest yield is obtained for the fragment combination 108^Ru+4^He+132^Te, which contain near doubly magic nuclei 132^Te (N=80, Z=52). In the case of 246^Cf and 248^Cf isotopes, the highest yield is obtained for the fragment combinations with near doubly magic nuclei 134^Te (N=82, Z=52) as the heavier fragment. The highest yield obtained for 250^Cf, 252^Cf, 254^Cf, 256^Cf, 258^Cf and 260^Cf isotopes is for the fragment combination with doubly magic nuclei 132^Sn (N=82, Z=50) as the heavier fragment. We have included the effect of deformation and orientation of fragments and this has revealed that in addition to closed shell effect, ground state deformation also plays an important role in the calculation of relative yield of fav...

  14. Increased postexercise insulin sensitivity is accompanied by increased AS160 phosphorylation in slow-twitch soleus muscle.

    Science.gov (United States)

    Iwabe, Maiko; Kawamoto, Emi; Koshinaka, Keiichi; Kawanaka, Kentaro

    2014-12-01

    A single bout of exercise can enhance insulin-stimulated glucose uptake in both fast-twitch (type II) and slow-twitch (type I) skeletal muscle for several hours postexercise. Akt substrate of 160 kDa (AS160) is most distal insulin signaling proteins that have been proposed to contribute to the postexercise enhancement of insulin action in fast-twitch muscle. In this study, we examined whether the postexercise increase in insulin action of glucose uptake in slow-twitch muscle is accompanied by increased phosphorylation of AS160 and its paralog TBC1D1. Male Wistar rats (~1-month-old) were exercised on a treadmill for 180 min (9 m/min). Insulin (50 μU/mL)-stimulated glucose uptake was increased at 2 h after cessation of exercise in soleus muscle composed of predominantly slow-twitch fibers. This postexercise increase in insulin action of glucose uptake was accompanied by increased phosphorylation of AS160 (detected by phospho-Thr642 and phospho-Ser588 antibody). On the other hand, prior exercise did not increase phosphorylation of TBC1D1 (detected by phospho-Thr590) at 2 h postexercise. These results suggest the possibility that an enhancement in AS160 phosphorylation but not TBC1D1 phosphorylation is involved with increased postexercise insulin action of glucose uptake in slow-twitch muscle.

  15. The Clinical Research in Advanced Stage Gastric Cancer Accompanying Hemorrhage with EHLF Chemotherapy and Thrombin to Take Orally

    Institute of Scientific and Technical Information of China (English)

    Chen Huoguo

    2003-01-01

    Purpose:Study therapeutic effectiveness about EHLF chemotherapy and thrombin to take orally in advanced stage gastriccancer accompanying hemorrhage.Methods:76 cases were divided into two groups at random,that were diagnosed by pathology andclinical. Research group included medicine:(etoposide)VP-16,100 mg intravenous drip,d1~3;(hydroxycamptothecin)HCPT, 10mg,ivtravenous drip,d1~5;(calciumfolinate)CF, 100 mg,intravenous drip,d1~5;(fluorouracil)5-Fu,0.5,intravenous drip,d1~5;thrombin,5oou,oral administration,three times in a day, d1~7.Matched control:normal regulation to medicine in gastric hemorrhye.Results:Effective rate in research group was 78.4%,obvious better than one in matched control 28.2%(P<0.01),bad reaction wasslight, life quantity was obvious exaltation.Conclusion:EHLF chemotherapy and thrombin to take orally was used as treatment project inadranced stage gastric cancer accompanying hemorrhage,good result in research group. It is worthy to deserve further reseach.

  16. Loss of Dok-1 and Dok-2 in mice causes severe experimental colitis accompanied by reduced expression of IL-17A and IL-22

    Energy Technology Data Exchange (ETDEWEB)

    Waseda, Masazumi; Arimura, Sumimasa [Division of Genetics, Department of Cancer Biology, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108-8639 (Japan); Shimura, Eri [Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108-8639 (Japan); Nakae, Susumu [Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108-8639 (Japan); Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Saitama, 332-0012 (Japan); Yamanashi, Yuji, E-mail: yyamanas@ims.u-tokyo.ac.jp [Division of Genetics, Department of Cancer Biology, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108-8639 (Japan)

    2016-09-09

    Appropriate immune responses and mucosal barrier functions are required for the maintenance of intestinal homeostasis. Defects in this defense system may lead to inflammatory disorders such as inflammatory bowel disease. Downstream of tyrosine kinases 1 (Dok-1) and its closest homolog, Dok-2, are preferentially expressed in immune cells, and play essential roles in the negative regulation of multiple signaling pathways in both innate and adaptive immunity. However, the function of these proteins in intestinal homeostasis remained unclear. Here we show that Dok-1/-2 double knockout (DKO) mice were highly susceptible to dextran sodium sulfate (DSS)-induced colitis compared with Dok-1 or Dok-2 single KO and wild type (WT) mice. Furthermore, DSS-treated Dok-1/-2 DKO mice exhibited increased colonic tissue damage accompanied by reduced proliferation of the epithelial cells relative to WT controls, suggesting that Dok-1/-2 DKO mice have defects in the repair of intestinal epithelial lesions. In addition, the levels of the Th17 cytokines IL-17A and IL-22, which have protective roles in DSS-induced colitis, were reduced in DSS-treated Dok-1/-2 DKO mice compared with WT mice. Taken together, our results demonstrate that Dok-1 and Dok-2 negatively regulate intestinal inflammation, apparently through the induction of IL-17A and IL-22 expression. - Highlights: • Dok-1 and Dok-2 play a cooperative role in protection against DSS-induced colitis. • Dok-1/-2 double KO (DKO) mice show extensive ulceration of the colon after DSS treatment. • Proliferation of colonic epithelium is inhibited in DSS-treated Dok-1/-2 DKO mice. • Expression of IL-17A and IL-22 is reduced in the colon of DSS-treated Dok-1/-2 DKO mice.

  17. Colonic inflammation accompanies an increase of β-catenin signaling and Lachnospiraceae/Streptococcaceae bacteria in the hind gut of high-fat diet-fed mice.

    Science.gov (United States)

    Zeng, Huawei; Ishaq, Suzanne L; Zhao, Feng-Qi; Wright, André-Denis G

    2016-09-01

    Consumption of an obesigenic/high-fat diet (HFD) is associated with a high colon cancer risk and may alter the gut microbiota. To test the hypothesis that long-term high-fat (HF) feeding accelerates inflammatory process and changes gut microbiome composition, C57BL/6 mice were fed HFD (45% energy) or a low-fat (LF) diet (10% energy) for 36 weeks. At the end of the study, body weights in the HF group were 35% greater than those in the LF group. These changes were associated with dramatic increases in body fat composition, inflammatory cell infiltration, inducible nitric oxide synthase protein concentration and cell proliferation marker (Ki67) in ileum and colon. Similarly, β-catenin expression was increased in colon (but not ileum). Consistent with gut inflammation phenotype, we also found that plasma leptin, interleukin 6 and tumor necrosis factor α concentrations were also elevated in mice fed the HFD, indicative of chronic inflammation. Fecal DNA was extracted and the V1-V3 hypervariable region of the microbial 16S rRNA gene was amplified using primers suitable for 454 pyrosequencing. Compared to the LF group, the HF group had high proportions of bacteria from the family Lachnospiraceae/Streptococcaceae, which is known to be involved in the development of metabolic disorders, diabetes and colon cancer. Taken together, our data demonstrate, for the first time, that long-term HF consumption not only increases inflammatory status but also accompanies an increase of colonic β-catenin signaling and Lachnospiraceae/Streptococcaceae bacteria in the hind gut of C57BL/6 mice.

  18. Research on Accompanying Cars Recognition in Practical Application%伴随车检测技术应用研究

    Institute of Scientific and Technical Information of China (English)

    赵新勇; 安实

    2012-01-01

    In massive dynamic traffic flows, it is very common to see the cars moving in a queue. In some scenarios, these cars are regarded as accompanying cars and suspected of gang crime support each other when that condition occurs in a high rate. It is very important to identify the accompanying vehicles as early as possible and to reduce potential risks of road traffic system and to reduce road-related public security cases and criminal cases. Based on the automatic license plate recognition database and data mining technology, this paper proposes a set of algorithms in identifying accompanying cars and a field test is conducted. The results demonstrate the performance of the algorithm with effectiveness, low detection error, wide application and capability for further investigation.%海量动态交通流中,经常出现结伴而行的车辆.特定区域内,当结伴车辆出现的概率较大时,即可将其视为伴随车辆,这类车辆具有相互掩护和团伙作案的重大嫌疑.及早检测和识别伴随车辆,能有效降低道路交通安全系统中的危险因素,对预防和减少与道路有关的治安和刑事案件,也具有十分重要的意义.本文在车牌自动识别数据库基础上,应用数据挖掘技术,提出伴随车辆检测和识别算法,并进行了实地测试.实验结果表明:应用数据挖掘技术对伴随车辆进行分析检测,具有检测效率高、检测误差小、应用范围广的特点,完全可以满足刑侦等部门对伴随嫌疑车辆进一步排查的需要.

  19. Changes in Neuronal Oscillations Accompany the Loss of Hippocampal LTP that Occurs in an Animal Model of Psychosis

    Science.gov (United States)

    Kalweit, Alexander N.; Amanpour-Gharaei, Bezhad; Colitti-Klausnitzer, Jens; Manahan-Vaughan, Denise

    2017-01-01

    The first-episode of psychosis is followed by a transient time-window of ca. 60 days during which therapeutic interventions have a higher likelihood of being effective than interventions that are started with a greater latency. This suggests that, in the immediate time-period after first-episode psychosis, functional changes occur in the brain that render it increasingly resistant to intervention. The precise mechanistic nature of these changes is unclear, but at the cognitive level, sensory and hippocampus-based dysfunctions become increasingly manifest. In an animal model of first-episode psychosis that comprises acute treatment of rats with the irreversible N-methyl-D-aspartate receptor (NMDAR)-antagonist, MK801, acute but also chronic deficits in long-term potentiation (LTP) and spatial memory occur. Neuronal oscillations, especially in the form of information transfer through θ and γ frequency oscillations are an intrinsic component of normal information processing in the hippocampus. Changes in θ-γ coupling and power are known to accompany deficits in hippocampal plasticity. Here, we examined whether changes in δ, θ, α, β and γ oscillations, or θ-γ coupling accompany the chronic loss of LTP that is observed in the MK801-animal model of psychosis. One and 4 weeks after acute systemic treatment of adult rats with MK801, a potent loss of hippocampal in vivo LTP was evident compared to vehicle-treated controls. Overall, the typical pattern of θ-γ oscillations that are characteristic for the successful induction of LTP was altered. In particular, θ-power was lower and an uncoupling of θ-γ oscillations was evident in MK801-treated rats. The alterations in network oscillations that accompany LTP deficits in this animal model may comprise a mechanism through which disturbances in sensory information processing and hippocampal function occur in psychosis. These data suggest that the hippocampus is likely to comprise a very early locus of functional

  20. Study of Auger decay process following multielectron excitation accompanying F 1s photoionization of CF{sub 4}

    Energy Technology Data Exchange (ETDEWEB)

    Machida, M. [Department of Material Science, Himeji Institute of Technology, Kamigori, Hyogo 678-1297 (Japan); RIKEN/SPring-8, Harima Institute, 1-1-1 Kouto, Mikazuki, Sayo, Hyogo 679-5148 (Japan); Tamenori, Y. [JASRI/SPring-8, 1-1-1, Kouto, Mikazuki, Sayo, Hyogo 679-5198 (Japan); Oura, M. [RIKEN/SPring-8, Harima Institute, 1-1-1 Kouto, Mikazuki, Sayo, Hyogo 679-5148 (Japan)]. E-mail: oura@spring8.or.jp; Mukoyama, T. [Kansai Gaidai University, 16-1 Nakamiya-Higashino-cho, Hirakata, Osaka 573-1001 (Japan)

    2005-06-15

    Auger decay process following multielectron excitation accompanying F 1s photoionization of CF{sub 4} molecule has been studied in the excitation energy range between 700 and 750-bar eV using angle-resolved Auger electron spectroscopy. The observed spectra have shown an enhancement in the specific band, at an electron kinetic energy of {approx} 644-bar eV, only in the spectrum recorded at 710-bar eV excitation energy of horizontally polarized light. This feature is discussed in terms of the resonant Auger emission originated from the doubly excited state. The evolution of spectral shape has also exhibited the excitation energy dependence above the threshold of multiple excitation. The origin of the spectral variation is considered due to gradual growth of the satellite Auger lines originated from the multielectron excitation.

  1. Emotional fluctuations in Bob Dylan's lyrics measured by the dictionary of affect accompany events and phases in his life.

    Science.gov (United States)

    Whissell, Cynthia

    2008-04-01

    Lyrics for Bob Dylan's songs between 1962 and 2001 (close to 100,000 words) were scored with the help of the Dictionary of Affect in Language (Whissell, 2006). Means for Pleasantness, Activation, and Imagery are reported for 22 Blocks characterizing this time span. Significant but weak differences across Blocks were found for all three measures at the level of individual words. Emotional fluctuations in words included in Bob Dylan's lyrics accompanied events and phases in his life, although they were not entirely dictated by these events. Dylan used more highly Imaged and more Active words at times when his work was critically acclaimed. More Passive word choices characterized times of prolonged stress, and more Pleasant choices times of experimentation. Dylan's three popularity peaks were used to divide the singer's career into three stages (rhetor, poet, sage) which differed in terms of pronouns used.

  2. [Separation in the mother-son relationship: an adolescent problem in Rainer Maria Rilke's story "Silent accompaniment"].

    Science.gov (United States)

    Wittstruck, W

    1995-01-01

    The story 'Quiet Accompanying' by Ranier Maria Rilke's early work is of special interest for research on adolescence. The story is a literary contribution to the separation conflict of parents and their maturing children, especially the separation crisis in the mother-son relationship. The story's suspense is derived from the son's desire to begin a new phase in life and his experience of being powerless regarding his ties to his family. Thereby the adolescent crisis of the male main character is indirectly picked out as the main theme in the description of the mother's separation crisis. In the mother's 'dream' where the departure for a sunday excursion is described, an image of the son's adolescence with the components search for identity, separation and closeness develops. The son's efforts finally fail because mother and son are captured in their relationship characterized by conflicts and fears.

  3. Strongyloidiasis in a diabetic patient accompanied by gastrointestinal stromal tumor: cause of eosinophilia unresponsive to steroid therapy.

    Science.gov (United States)

    Won, Eun Jeong; Jeon, Jin; Koh, Young-Il; Ryang, Dong Wook

    2015-04-01

    We report here a case of strongyloidiasis in a 72-year-old diabetic patient (woman) accompanied by gastrointestinal stromal tumor receiving imatinib therapy, first diagnosed as hypereosinophilic syndrome and treated with steroids for uncontrolled eosinophilia. She suffered from lower back pain and intermittent abdominal discomfort with nausea and diagnosed with gastrointestinal stromal tumor. After post-operative imatinib treatment eosinophilia persisted, so that steroid therapy was started under an impression of hypereosinophilic syndrome. In spite of 6 months steroid therapy, eosinophilia persisted. Stool examination was performed to rule out intestinal helminth infections. Rhabditoid larvae of Strongyloides stercoralis were detected and the patient was diagnosed as strongyloidiasis. This diagnosis was confirmed again by PCR. The patient was treated with albendazole for 14 days and her abdominal pain and diarrhea improved. This case highlights the need for thorough investigation, including molecular approaches, to test for strongyloidiasis before and during steroid therapies.

  4. Congenital peripheral developing odontoma accompanied by congenital teratomatous fibroma in a 9-month-old boy: a case report.

    Science.gov (United States)

    Mikami, Toshinari; Yagi, Masaatsu; Mizuki, Harumi; Takeda, Yasunori

    2013-03-01

    Peripheral odontoma is rare, and only two cases of congenital peripheral odontoma have been reported. Congenital oral fibroma is also rare. We describe a unique case of congenital peripheral developing odontoma accompanied by congenital teratomatous fibroma in an infant. Both tumors were difficult to detect on radiography. Two small masses were seen in the median anterior portion of the palatal mucosa of a 9-month-old boy. The masses had been present since birth and were surgically removed at age 28 months, when one of the masses had grown to a diameter of 8 mm. Histopathologic examination showed a fibrous lesion and a tooth germ-like rounded lesion composed of dental papilla, enamel organ, dentin, and cementum. Although congenital odontoma is rare, it should be considered when selecting appropriate treatment, as early radiographic detection is difficult.

  5. An ecologic accompanying research on the fleet test electromobility. Final report; Oekologische Begleitforschung zum Flottenversuch Elektromobilitaet. Endbericht

    Energy Technology Data Exchange (ETDEWEB)

    Helms, Heinrich; Lambrecht, Udo; Joehrens, Julius; Pehnt, Martin; Liebich, Axel; Weiss, Uta; Kaemper, Claudia

    2013-06-15

    Mobility is a prerequisite for numerous economic and private activities and thus a central component of our life. The demand for mobility in Germany is predominantly covered by the road traffic. The Institute for Energy and Environmental Research (Heidelberg, Federal Republic of Germany) carried out an ecologic accompanying research in the course of a fleet test in order to determine the potentials of environmental improvement of the vehicles tested in a field test. These potentials were perpetuated for future serial vehicles and projected to Germany. The main themes of this contribution are: (a) Production and disposal of plug-in-hybrids; (b) The TwinDrive in the fleet test; (c) Plug-in-hybrid serial vehicles 2020; (d) Perspective of electromobility in Germany 2030; (e) Strategic evaluation of the electomobility.

  6. Plasma sheet stretching accompanied by field aligned energetic ion fluxes observed by the MUADU instrument aboard TC-2

    Institute of Scientific and Technical Information of China (English)

    Lu Li; S.MCKENNA-LAWLOR; S.BARABASH; LIU ZhenXing; CAO JinBin; J.BALAZ; K.KUDELA; T.L.ZHANG; C.M.CARR

    2007-01-01

    The NUADU(NeUtral Atom Detector Unit)instrument aboard TC-2 recorded 4π solid angle images of charged particles(E>180 keV)spiraling around the magnetic field lines in the near-Earth plasma sheet (at~-7 RE,equatorial dawn-to-night side)during a geomagnetic storm(Dst=-219 nT)on August 24,2005.Energetic ion beam events characterized by symmetrical,ring-like,solid angle distributions around ambient magnetic field lines were observed during a 34-minute traversal of the plasma sheet by the TC-2 spacecraft.Also,observations during these multiple crossings of the plasma sheet were monitored by the magnetometer experiment(FGM)aboard the same spacecraft.During each crossing,a whistler-mode chorus enhancement was observed in the anisotropic area by the TC-2 low frequency electromagnetic wave detector(LFEW/TC-2)at a frequency just above that of the local lower hybrid wave.A comparison of the ion pitch angle distribution(PAD)map with the ambient magnetic field shows that an enhancement in the field aligned energetic ion flux was accompanied by tailward stretching of the magnetic field lines in the plasma sheet.In contrast,the perpendicular ion-flux enhancement was accompanied by a signature indicating the corresponding shrinkage of the magnetic field lines in the plasma sheet.Since both parallel ion-flux and perpendicular ion-flux enhancements occurred intermittently,the data were interpreted to imply a dynamical,oscillatory process of the magnetic field line(stretching and shrinking)in the near-Earth plasma sheet,which might have acted to help establish an interaction region in this area which would support continuous aurora-substorm triggering during the ongoing magnetic storm.The whistler-mode chorus may have been produced due to ion gyro-resonance during particle pitch angle diffusion after the plasma sheet compression.

  7. Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice

    Science.gov (United States)

    Li, Siyuan; Xia, Qiong; Wang, Fang; Yu, Xiaoming; Ma, Jian; Kou, Hongping; Lin, Xiuyun; Gao, Xiang; Liu, Bao

    2017-01-01

    DNA methylation is an integral component of the epigenetic code in most higher eukaryotes. Exploring the extent to which DNA methylation can be altered under a specific condition and its heritability is important for elucidating the biological functions of this epigenetic modification. Here, we conducted MSAP analysis of rice plants with altered phenotypes subsequent to a low-dose Nd3+YAG laser irradiation. We found that all four methylation patterns at the 5′-CCGG sites that are analyzable by MSAP showed substantial changes in the immediately treated M0 plants. Interestingly, the frequencies of hypo- and hypermethylation were of similar extents, which largely offset each other and render the total methylation levels unchanged. Further analysis revealed that the altered methylation patterns were meiotically heritable to at least the M2 generation but accompanied with further changes in each generation. The methylation changes and their heritability of the metastable epigenetic state were verified by bisulfite sequencing of portion of the retrotranspon, Tos17, an established locus for assessing DNA methylation liability in rice. Real-time PCR assay indicated that the expression of various methylation-related chromatin genes was perturbed, and a Pearson correlation analysis showed that many of these genes, especially two AGOs (AGO4-1 and AGO4-2), were significantly correlated with the methylation pattern alterations. In addition, excisions of a MITE transposon, mPing, occurred rampantly in the laser irradiated plants and their progenies. Together, our results indicate that heritable DNA methylation changes can be readily induced by low-dose laser irradiation, and which can be accompanied by transpostional activation of transposable elements.

  8. Comparative study of the therapeutic effects of corticosteroid injection accompanied by casting and heel pad in treatment of heel pain

    Directory of Open Access Journals (Sweden)

    Mehdi Motififard

    2008-08-01

    Full Text Available

    • BACKGROUND: Heel pain is a common condition in adults that may cause significant discomfort and disability. This study was conducted to evaluate the therapeutic effects of corticosteroid injection accompanied by casting, compared with heel pad utilization in treatment of heel pain.
    • METHODS: In this randomized clinical trial study, among patients referred to orthopedic clinics in Isfahan, Iran, from December 2004 to December 2006, 90 patients with heel pain were enrolled. They were randomly assigned into two groups. Heel pad was the only therapeutic method utilized in group A for six months. Corticosteroid injection accompanied by casting was performed for patients in group B. The evaluated information was compared before and after treatment.
    •  RESULTS: Among patients in group A, after 6 months of heel pad utilization, heel pain was completely improved in 3 patients (6.7%, relatively decreased in 22 patients (48.9% and didn't change in 20 patients (44.4%. However, among patients in group B, heel pain was completely improved in 11 patients (24.5%, decreased in 29 patients (64.4% and didn't change in 5 patients (11.1% after the treatment completed.
    • CONCLUSIONS: It seemed that corticosteroid injection in companion with casting was a more effective therapeutic method in heel pain treatment than heel pad utilization.
    • KEYWORDS: Corticosteroid injection, casting, heel pad, heel pain.

  9. High-Bandwidth Atomic Force Microscopy Reveals A Mechanical spike Accompanying the Action Potential in mammalian Nerve Terminals

    Science.gov (United States)

    Salzberg, Brian M.

    2008-03-01

    Information transfer from neuron to neuron within nervous systems occurs when the action potential arrives at a nerve terminal and initiates the release of a chemical messenger (neurotransmitter). In the mammalian neurohypophysis (posterior pituitary), large and rapid changes in light scattering accompany secretion of transmitter-like neuropeptides. In the mouse, these intrinsic optical signals are intimately related to the arrival of the action potential (E-wave) and the release of arginine vasopressin and oxytocin (S-wave). We have used a high bandwidth (20 kHz) atomic force microscope (AFM) to demonstrate that these light scattering signals are associated with changes in nerve terminal volume, detected as nanometer-scale movements of a cantilever positioned on top of the neurohypophysis. The most rapid mechanical response, the ``spike'', has duration comparable to that of the action potential (˜2 ms) and probably reflects an increase in terminal volume due to H2O movement associated with Na^+-influx. Elementary calculations suggest that two H2O molecules accompanying each Na^+-ion could account for the ˜0.5-1.0 å increase in the diameter of each terminal during the action potential. Distinguishable from the mechanical ``spike'', a slower mechanical event, the ``dip'', represents a decrease in nerve terminal volume, depends upon Ca^2+-entry, as well as on intra-terminal Ca^2+-transients, and appears to monitor events associated with secretion. A simple hypothesis is that this ``dip'' reflects the extrusion of the dense core granule that comprises the secretory products. These dynamic high bandwidth AFM recordings are the first to monitor mechanical events in nervous systems and may provide novel insights into the mechanism(s) by which excitation is coupled to secretion at nerve terminals.

  10. Accompanying monitoring. Pt. I. Energy consumption and room climate in the passive house kindergarten; Begleitendes Monitoring. T. 1. Energieverbrauch und Raumklima im Passivhauskindergarten

    Energy Technology Data Exchange (ETDEWEB)

    Trogisch, Achim; Berbig, Alex [HTW Dresden (DE). Lehrgebiet Technische Gebaeudeausruestung (TGA); Herrmann, Hendrik [Ingenieurbuero Dr. Scheffler und Partner, Dresden (Germany)

    2011-07-01

    The kindergarten 'Rainbow' in Dresden-Klotzsche (Federal Republic of Germany) was designed, planned, implemented and commissioned in October 2009 according to the passive house standard. An accompanying monitoring shows a successful design and implementation as well.

  11. Roflumilast, ursodeoxycholic acid and nucleinate efficiency in patients with chronic obstructive pulmonary disease with accompanying chronic non-stone cholecystitis.

    Directory of Open Access Journals (Sweden)

    Dudka T.V.

    2014-11-01

    Full Text Available Global Initiative for diagnosis, treatment and prevention of COPD has recently included roflumilast (R - an inhibitor of phosphodiesterase-4 (PDE-4 as a new therapeutic agent to the recommendations for the management of patients suffering from COPD with severe and moderate course. Objective. To investigate the efficacy of roflumilast, ursodeoxycholic and ribonucleinic acids in patients with COPD with an accompanying chronic non-stone cholecystitis. Materials and methods. The study involved 40 patients with COPD stage II-III, in the acute phase with an accompanying CNC in the acute phase and 20 practically healthy individuals (PHI. Patients of the control group (group 2 received berodual, UA500 mg overnight for 30 days, under the conditions of infective exacerbation of COPD - antibiotic therapy. Group 1 (study, 20 people received roflumilast 500 mg additionally once a day, nucleinas 500 mg 3 times daily for 30 days. Results and discussion. The results obtained in the study of the dynamics of treatment and their analysis indicate that exposure to complex therapy, amelioration, reducing the signs of exacerbation of COPD and CNC, a significant improvement of quality of life in patients of group 1 were noticed sooner. Figures of external respiration functions(FER in dynamics of treatment in patients with COPD with an accompanying CNC show higher efficiency of the proposed therapy too. In particular, the rate of forced expiration for the first second after treatment in patients of group 1 increased by 31,5% (p<0,05, while patients in group 2 - by 14,0% (p<0,05 probable presence of intergroup differences (p<0,05. Taking into consideration the fact that the treatment of patients of group 1 included antioxidant preparation H - dynamic performance and the intensity of lipid peroxidation were significantly different from baseline in all periods of observation. For instance, the content of MA (malonic aldehyde in plasma after treatment in group 1 decreased

  12. Acinar-to-ductal metaplasia accompanies c-myc-induced exocrine pancreatic cancer progression in transgenic rodents.

    Science.gov (United States)

    Grippo, Paul J; Sandgren, Eric P

    2012-09-01

    Several important characteristics of exocrine pancreatic tumor pathogenesis remain incompletely defined, including identification of the cell of origin. Most human pancreatic neoplasms are ductal adenocarcinomas. However, acinar cells have been proposed as the source of some ductal neoplasms through a process of acinar-to-ductal metaplasia. The oncogenic transcription factor c-myc is associated with human pancreatic neoplasms. Transgenic mice overexpressing c-myc under control of acinar cell-specific elastase (Ela) gene regulatory elements not only develop acinar cell carcinomas but also mixed neoplasms that display both acinar-like neoplastic cells and duct-like neoplastic cells. In this report, we demonstrate that, first, c-myc is sufficient to induce acinar hyperplasia, though neoplastic lesions develop focally. Second, cell proliferation remains elevated in the neoplastic duct cell compartment of mixed neoplasms. Third, the proliferation/apoptosis ratio in cells from all lesion types remains constant, suggesting that differential regulation of these processes is not a feature of cancer progression in this model. Fourth, before the development of mixed neoplasms, there is transcriptional activation of the duct cell-specific cytokeratin-19 gene promoter in multicellular foci of amylase-positive acinar neoplasms. This observation provides direct evidence for metaplasia as the mechanism underlying development of ductal neoplastic cells within the context of an acinar neoplasm and suggests that the stimulus for this transformation acts over a multicellular domain or field within a neoplasm. Finally, focal ductal elements develop in some acinar cell carcinomas in Ela-c-myc transgenic rats, indicating that myc-associated acinar-to-ductal metaplasia is not restricted to the mouse.

  13. Protective effect of a bacterial extract against acute exacerbation in patients with chronic bronchitis accompanied by chronic obstructive pulmonary disease

    Institute of Scientific and Technical Information of China (English)

    李靖; 郑劲平; 袁锦屏; 曾广翘; 钟南山; 林材元

    2004-01-01

    Background Immunostimulating agents made from bacterial extracts represent a class of medications that contains antigens derived from several bacterial strains and their potential ability to prevent bacterial infections results from the stimulation of the nonspecific component of the immune system. The present study investigated the effect of the oral immunostimulant Broncho-Vaxom, which includes material from eight different species of bacteria that are frequently present in the lower respiratory tract, on the frequency and severity of acute exacerbation in patients with chronic bronchitis accompanied by chronic obstructive pulmonary disease (COPD). Methods Ninety patients with chronic bronchitis complicated with COPD were randomly divided into groups A and B. Forty-nine subjects in group A received oral capsules containing 7mg Broncho-Vaxom, while 41 patients in group B received similar placebo capsules. Both groups took one capsule daily for the first 10 days of each month for 3 consecutive months. The frequency of acute exacerbation, symptom scores, and lung function were recorded for the following one year period.Results There was a significant decrease in the incidence, duration, and severity of acute exacerbation, as well as a reduction in the course of antibiotics administered and in the dosage of bronchodilator and mucolytic agent in group A, as compared to group B (P<0.05, respectively). Symptom scores for cough, sputum, dyspnea, as well as symptoms observed upon auscultation of the chest also improved significantly in group A as compared to group B (P<0.05, respectively). The bacterial clearance rate in sputum cultures from patients who received no antibiotics for the first 3 months was also significantly higher in group A compared to group B (P<0.01).Conclusions Orally administered Broncho-Vaxom is associated with a decrease in the incidence of acute exacerbation and a decrease in the need for antibiotics and symptomatic relief medications in patients

  14. Superior labrum anterior to posterior lesion type II with accompanied findings: assessment of shoulder MR arthrographic findings

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Sun Young; Chun, Kyung Ah; Kwon, Oh Soo; Kim, Ki Tae [The Catholic University of Korea, Uijeongbu St. Mary' s Hospital, Uijeongbu (Korea, Republic of)

    2006-12-15

    To describe the pattern of various shoulder abnormalities with an associated superior labrum anterior to posterior (SLAP) lesion type II using magnetic resonance (MR) arthrography, and to assess the clinical significance of the associated abnormalities. A retrospective review of the MR arthrographic findings of 92 cases of a shoulder with an arthroscopically confirmed SLAP lesion type II was performed. The MR arthrography images were reviewed and analyzed. MR arthrographic analysis noted the presence of a rotator cuff abnormality, acromioclavicular arthritis, adhesive capsulitis, glenohumeral arthritis, a labral abnormality besides the SLAP lesion, and a paralabral cyst. The patients with SLAP lesions were divided into two age groups: those over 40 years of age and those forty years old or younger. Statistical analysis was performed to evaluate the influence of age on the various shoulder abnormalities with associated SLAP lesion. Of the 92 SLAP lesions type II, there were 7 cases (8%) of isolated SLAP lesions without any associated any shoulder abnormality. Eighty-five (92%) SLAP lesions were associated with various shoulder abnormalities including rotator cuff tendinosis (30/92, 33%), partial-thickness tear (36/92, 39%), full-thickness tear (2/92, 2%), acromioclavicular arthritis (46/92, 50%), adhesive capsulitis (7/92, 8%), glenohumeral arthritis (15/92, 16%), labral abnormality (26/92, 28%) and paralabral cyst (7/92, 8%). The SLAP lesions (60/92, 65%) in patients over forty years of age were accompanied by a significantly high number of rotator cuff abnormalities ({rho} < 0.001), glenohumeral osteoarthritis ({rho} = 0.001), and acromioclavicular osteoarthritis ({rho} < 0.001). In contrast, the SLAP lesions (32/92, 35%) in patients forty years old or younger had a significantly high number of anterior or posterior labral lesions ({rho} < 0.001). Isolated SLAP lesions type II without other associated shoulder abnormalities are uncommon, and the age of the patient

  15. Process for control of cell division

    Science.gov (United States)

    Cone, C. D., Jr. (Inventor)

    1977-01-01

    A method of controlling mitosis of biological cells was developed, which involved inducing a change in the intracellular ionic hierarchy accompanying the cellular electrical transmembrane potential difference (Esubm) of the cells. The ionic hierarchy may be varied by imposing changes on the relative concentrations of Na(+), K(+) and Cl(-), or by directly imposing changes in the physical Esubm level across the cell surface.

  16. Chemoprevention of colorectal cancer by grape seed proanthocyanidin is accompanied by a decrease in proliferation and increase in apoptosis.

    Science.gov (United States)

    Nomoto, Hiroshi; Iigo, Masaaki; Hamada, Hiroki; Kojima, Shuji; Tsuda, Hiroyuki

    2004-01-01

    Effects of proanthocyanidin (PA), procyanidin B-2 (B-2), and epigallocatechin gallate (EGCG) on azoxymethane (AOM)-induced colonic preneoplastic aberrant crypt foci (ACF) formation were investigated using F344 rats. The numbers of total ACF in rats treated with 0.002% PA and 0.05% B-2 were significantly decreased compared with the AOM alone group (control). Cell proliferation in the colon, as shown by proliferating cells nuclear antigen (PCNA), was also reduced in those treatments. The single-stranded DNA (ssDNA) labeling index, a marker for apoptosis, was significantly increased in 0.002% PA and 0.05% B-2 groups compared with control. Moreover, the numbers of CD11b/c+ cells (macrophages) and NKR-P1A+ cells (NK cells) in the all groups were significantly increased compared with control. In an in vitro study using rat colon cancer cell line RCN-9, PA, especially 5-10mer of PA (PA5/10), strong growth inhibition was shown. PA5/10 caused the most remarkable apoptosis as cleared by FACS analysis. These cells showed significantly increased caspase-3 activity. The results would suggest that the PA, especially PA5/10, might strongly enhance caspase-3 activity and cause apoptosis in cancer cells. PA at fairly low doses in the long term might serve as an effective means for preventing colon carcinogenesis.

  17. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture.

    Science.gov (United States)

    Barta, Tomas; Dolezalova, Dasa; Holubcova, Zuzana; Hampl, Ales

    2013-03-01

    Cell cycle represents not only a tightly orchestrated mechanism of cell replication and cell division but it also plays an important role in regulation of cell fate decision. Particularly in the context of pluripotent stem cells or multipotent progenitor cells, regulation of cell fate decision is of paramount importance. It has been shown that human embryonic stem cells (hESCs) show unique cell cycle characteristics, such as short doubling time due to abbreviated G1 phase; these properties change with the onset of differentiation. This review summarizes the current understanding of cell cycle regulation in hESCs. We discuss cell cycle properties as well as regulatory machinery governing cell cycle progression of undifferentiated hESCs. Additionally, we provide evidence that long-term culture of hESCs is accompanied by changes in cell cycle properties as well as configuration of several cell cycle regulatory molecules.

  18. Changes in regional activity are accompanied with changes in inter-regional connectivity during 4 weeks motor learning.

    Science.gov (United States)

    Ma, Liangsuo; Wang, Binquan; Narayana, Shalini; Hazeltine, Eliot; Chen, Xiying; Robin, Donald A; Fox, Peter T; Xiong, Jinhu

    2010-03-08

    Structural equation modeling (SEM) and fMRI were used to test whether changes in the regional activity are accompanied by changes in the inter-regional connectivity as motor practice progresses. Ten healthy subjects were trained to perform finger movement task daily for 4 weeks. Three sessions of fMRI images were acquired within 4 weeks. The changes in inter-regional connectivity were evaluated by measuring the effective connectivity between the primary motor area (M1), supplementary motor area (SMA), dorsal premotor cortex (PMd), basal ganglia (BG), cerebellum (CB), and posterior ventrolateral prefrontal cortex (pVLPFC). The regional activities in M1 and SMA increased from pre-training to week 2 and decreased from week 2 to week 4. The inter-regional connectivity generally increased in strength (with SEM path coefficients becoming more positive or negative) as practice progressed. The increases in the strength of the inter-regional connectivity may reflect long-term reorganization of the skilled motor network. We suggest that the performance gain was achieved by dynamically tuning the inter-regional connectivity in the motor network.

  19. Comparison of radiography, computed tomography and magnetic resonance imaging in the detection of sacroiliitis accompanying ankylosing spondylitis

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Wei; Feng Feng; Yang Hongzen; Jiang Ming [Department of Radiology, Peking Union Medical College Hospital, Beijing (China); Dion, E.; Genant, H.K. [Department of Radiology, Musculoskeletal Section and Osteoporosis and Arthritis Research Group, University of California San Francisco, CA 94143-0628 (United States)

    1998-06-01

    Objective. To compare magnetic resonance (MR) imaging, computed tomography (CT), and radiography in the detection of sacroiliitis accompanying ankylosing spondylitis (AS). Design and subjects. Nine volunteers and 24 patients were recruited. Radiography, CT, and MR imaging were completed within a 1-week period in 24 patients with AS. In precontrast MR examination, spin-echo T1, fast spin-echo T2, and gradient echo with rephasing T2* images were obtained without fat saturation using a 0.3-T imager for all volunteers and patients. Postcontrast MR examination was performed using the same precontrast SE T1 sequence for patients with AS. Results and conclusions. MR imaging directly showed the normal cartilage in all 16 sacroiliac joints of the 8 volunteers. In the 24 patients with AS, cartilage abnormalities were observed in 42 sacroiliac joints. More diagnoses of sacroiliitis were made using MR and CT imaging than using radiography (P<0.001). Therefore, low-field-strength MR can be useful in detecting early sacroiliitis in patients with AS. MR imaging was able to reveal early cartilage changes and bone marrow edema, which could not be found by either CT or radiography. (orig.) With 5 figs., 4 tabs., 36 refs.

  20. Designing an accompanying ecosystem to foster entrepreneurship among agronomic and forestry engineering students. Opinion and commitment of university lecturers

    Science.gov (United States)

    Ortiz-Medina, L.; Fernández-Ahumada, E.; Lara-Vélez, P.; Taguas, E. V.; Gallardo-Cobos, R.; del Campillo, M. C.; Guerrero-Ginel, J. E.

    2016-07-01

    In the Higher School of Agronomic and Forestry Engineering of the University of Cordoba, a collective project conceived as an 'ecosystem to support and accompany entrepreneurs' has been proposed. The approach aims to spread and consolidate the entrepreneurial spirit and to respond to the demands of possible stakeholders involved in the whole process of training, as well as the subsequent integration of graduates into the labour market. Putting into practice this initiative, which involves multiple actors, is a complex and difficult task. For this reason, prior to its implementation, the authors considered it necessary to listen to main stakeholders' opinions and evaluate their degree of commitment and the requirements they consider important for the viability and sustainability of the initiative. This paper focuses on the faculty's opinions, gathered by means of a survey conducted with the entire faculty (N = 128, response rate = 45%) and semi-structured interviews held with 20 members of the School board. The results suggest that there is a general consensus on the suitability of this collective project and that there is a core of teachers willing to get involved. Evidently, guidelines need to be produced to facilitate taking on such tasks. However, the main drawbacks are related with the conflict between formal requirements of professor professional profile and the steps needed to establish the ecosystem.

  1. Preparation of AgCl Nano-Crystal Embedded Tellurite Nonlinear Optical Glasses under Electric Field Accompanied Heat Treatment

    Institute of Scientific and Technical Information of China (English)

    Jian LIN; Wenhai HUANG; Bofang LI; Chong JIN; Changcheng LIU; Shuhua LEI; Zhenrong SUN

    2008-01-01

    The quantum effect of nano-crystals is an important factor to improve nonlinear optical performance of nano-crystal embedded glasses,while controlling the size distribution and content of nano-crystals in the glass accurately is a key to obtain good quality.The auxiliary direct current electric field,accompanied with heat treatment,was applied on AgCl containing niobic tellurite glass sheet.The nucleation and crystallization of the glass were well controlled under auxiliary electric field.It was found that the average size of AgCl nano-crystal particles in the glass is smaller than that under single heat treatment,and the content of nano-crystals is higher.Therefore the third-order nonlinear optical performance of the glass was increased a lot.The local-area distributed AgCl nano-crystal particles can also be embedded into a glass sheet by using locally applied electric field.

  2. Clinical assessment of epidural analgesia induced by xylazine-lidocaine combination accompanied by xylazine sedation in calves

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    Kamiloglu Alkan

    2005-10-01

    Full Text Available The aim of the present study was to investigate whether epidural administration of a xylazine-lidocaine combination accompanied by xylazine sedation would provide satisfactory analgesia for some surgical procedures on 10 calves admitted to the Department of Veterinary Surgery, University of Kafkas with perineal urolithiasis (n:2, rectovaginal fistula (n:1, atresia ani (n:2, omphalophlebitis (n:2, omphaloarteritis (n:1 and umbilical hernia (n:2. Following intramuscular injection of xylazine at a dose of 0.05 mg/kg for sedation, xylazine-lidocaine combination (0.2 mg/kg lidocaine + 0.02 mg/kg xylazine + 5 ml 0.9% NaCl was administrated into the lumbosacral (L6-S1, sacrococcygeal (S5-Co1 or intercoccygeal (Co1-Co2 space. Heart rate, respiratory rate and rectal temperature were recorded prior to and during analgesia at 5, 10, 15, 30 and 60 minutes. Furthermore, depth and duration of analgesia were evaluated during surgical intervention. The study revealed that the combination of epidural xylazine-lidocaine with xylazine sedation was highly satisfactory for surgery of the lower urinary tract and the perineal region, but it was less so for surgery of the umbilical area.

  3. Clinical assessment of epidural analgesia induced by xylazine-lidocaine combination accompanied by xylazine sedation in calves

    Science.gov (United States)

    2005-01-01

    The aim of the present study was to investigate whether epidural administration of a xylazine-lidocaine combination accompanied by xylazine sedation would provide satisfactory analgesia for some surgical procedures on 10 calves admitted to the Department of Veterinary Surgery, University of Kafkas with perineal urolithiasis (n:2), rectovaginal fistula (n:1), atresia ani (n:2), omphalophlebitis (n:2), omphaloarteritis (n:1) and umbilical hernia (n:2). Following intramuscular injection of xylazine at a dose of 0.05 mg/kg for sedation, xylazine-lidocaine combination (0.2 mg/kg lidocaine + 0.02 mg/kg xylazine + 5 ml 0.9% NaCl) was administrated into the lumbosacral (L6-S1), sacrococcygeal (S5-Co1) or intercoccygeal (Co1-Co2) space. Heart rate, respiratory rate and rectal temperature were recorded prior to and during analgesia at 5, 10, 15, 30 and 60 minutes. Furthermore, depth and duration of analgesia were evaluated during surgical intervention. The study revealed that the combination of epidural xylazine-lidocaine with xylazine sedation was highly satisfactory for surgery of the lower urinary tract and the perineal region, but it was less so for surgery of the umbilical area. PMID:21851664

  4. Human papillomavirus-32-associated focal epithelial hyperplasia accompanying HPV-16-positive papilloma-like lesions in oral mucosa.

    Science.gov (United States)

    Liu, Na; Wang, Jiayi; Lei, Lei; Li, Yanzhong; Zhou, Min; Dan, Hongxia; Zeng, Xin; Chen, Qianming

    2013-05-01

    Human papillomavirus infection can cause a variety of benign or malignant oral lesions, and the various genotypes can cause distinct types of lesions. To our best knowledge, there has been no report of 2 different human papillomavirus-related oral lesions in different oral sites in the same patient before. This paper reported a patient with 2 different oral lesions which were clinically and histologically in accord with focal epithelial hyperplasia and oral papilloma, respectively. Using DNA extracted from these 2 different lesions, tissue blocks were tested for presence of human papillomavirus followed by specific polymerase chain reaction testing for 6, 11, 13, 16, 18, and 32 subtypes in order to confirm the clinical diagnosis. Finally, human papillomavirus-32-positive focal epithelial hyperplasia accompanying human papillomavirus-16-positive oral papilloma-like lesions were detected in different sites of the oral mucosa. Nucleotide sequence sequencing further confirmed the results. So in our clinical work, if the simultaneous occurrences of different human papillomavirus associated lesions are suspected, the multiple biopsies from different lesions and detection of human papillomavirus genotype are needed to confirm the diagnosis.

  5. The effects of ankle joint muscle strengthening and proprioceptive exercise programs accompanied by functional electrical stimulation on stroke patients' balance.

    Science.gov (United States)

    Kim, Kyunghoon; Lee, Sukmin; Kim, Donghoon; Kim, Kyou Sik

    2015-09-01

    [Purpose] The purpose of the present study was to examine the effects of ankle joint muscle strengthening and proprioceptive exercises accompanied by functional electrical stimulation on stroke patients' balance ability. [Methods] For six weeks beginning in April 2015, 22 stroke patients receiving physical therapy at K Hospital located in Gyeonggi-do were divided into a functional electrical stimulation (FES), ankle proprioceptive exercise and ankle joint muscle strengthening exercise group (FPS group) of 11 patients and an FES and stretching exercise group (FS group) of 11 patients. The stimulation and exercises were conducted for 30 min per day, five days per week for six weeks. Balance ability was measured using a BioRescue and the Berg balance scale, functional reach test, and the timed up-and-go test were also used as clinical evaluation indices. Repeated measures ANOVA was conducted to examine differences between before the exercises and at three and six weeks after beginning the exercises within each group, and the amounts of change between the two groups were compared. [Results] In the comparison within each group, both groups showed significant differences between before and after the experiment in all the tests and comparison between the groups showed that greater improvement was seen in all values in the FPS group. [Conclusion] In the present study, implementing FES and stretching exercises plus ankle joint muscle strengthening and proprioceptive exercises was more effective at improving stroke patients balance ability than implementing only FES and stretching exercises.

  6. Development and pilot testing of a questionnaire to determine the ability and willingness of health personnel accompanying perinatal bereavement

    Directory of Open Access Journals (Sweden)

    Mª José Domínguez Santarén

    2013-01-01

    Full Text Available Introduction. The care that parents receive around the time of a loss has a huge impact on their perception of what happened and on their ability to cope. Good care cannot remove the pain and devastation that the loss of a pregnancy or the death of a baby can bring, but can promote healing.Methodology: Creation and pilot study for a questionnaire to determinate the capacity and willingness of perinatal bereavement support from staff in hospitalization and delivery room services in Zaragoza and Jaca who care for couples with a perinatal death.Statistical analysis. Qualitative analysis is made of the difficulties and limitations of this support staff is performing. Psychometric tests were conducted to determine the reliability and validity of the questionnaire by calculating Cronbach´s alpha and the intraclass correlation coefficient. For the analysis of construct validity, we performed the principal components factorial analysis (PCFA through the Varimax rotation system.Results. The qualitative analysis of open-ended responses indicates a lack of knowledge about this type of mourning and social and communication tools that often precludes effective accompaniment. We obtained a Cronbach alpha value of 0.835 overall questionnaire, which indicates high internal consistency or coherence among the items and relatively high CCI indicates good stability over time with significance p<,001. Making appropriate modifications could assess the ability and willingness of workers.

  7. Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration.

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    Suzana Gispert

    Full Text Available BACKGROUND: Parkinson's disease (PD is an adult-onset movement disorder of largely unknown etiology. We have previously shown that loss-of-function mutations of the mitochondrial protein kinase PINK1 (PTEN induced putative kinase 1 cause the recessive PARK6 variant of PD. METHODOLOGY/PRINCIPAL FINDINGS: Now we generated a PINK1 deficient mouse and observed several novel phenotypes: A progressive reduction of weight and of locomotor activity selectively for spontaneous movements occurred at old age. As in PD, abnormal dopamine levels in the aged nigrostriatal projection accompanied the reduced movements. Possibly in line with the PARK6 syndrome but in contrast to sporadic PD, a reduced lifespan, dysfunction of brainstem and sympathetic nerves, visible aggregates of alpha-synuclein within Lewy bodies or nigrostriatal neurodegeneration were not present in aged PINK1-deficient mice. However, we demonstrate PINK1 mutant mice to exhibit a progressive reduction in mitochondrial preprotein import correlating with defects of core mitochondrial functions like ATP-generation and respiration. In contrast to the strong effect of PINK1 on mitochondrial dynamics in Drosophila melanogaster and in spite of reduced expression of fission factor Mtp18, we show reduced fission and increased aggregation of mitochondria only under stress in PINK1-deficient mouse neurons. CONCLUSION: Thus, aging Pink1(-/- mice show increasing mitochondrial dysfunction resulting in impaired neural activity similar to PD, in absence of overt neuronal death.

  8. Parasympathetic reinnervation accompanied by improved post-exercise heart rate recovery and quality of life in heart transplant recipients.

    Science.gov (United States)

    Imamura, Teruhiko; Kinugawa, Koichiro; Okada, Ikuko; Kato, Naoko; Fujino, Takeo; Inaba, Toshiro; Maki, Hisataka; Hatano, Masaru; Kinoshita, Osamu; Nawata, Kan; Kyo, Shunei; Ono, Minoru

    2015-01-01

    Although sympathetic reinnervation is accompanied by the improvement of exercise tolerability during the first years after heart transplantation (HTx), little is known about parasympathetic reinnervation and its clinical impact. We enrolled 21 recipients (40 ± 16 years, 71% male) who had received successive cardiopulmonary exercise testing at 6 months, and 1 and 2 years after HTx. Exercise parameters such as peak oxygen consumption or achieved maximum load remained unchanged, whereas recovery parameters including heart rate (HR) recovery during 2 minutes and the delay of peak HR, which are influenced by parasympathetic activity, improved significantly during post-HTx 2 years (P Heart Failure (HF) Questionnaire at post-HTx 6 months and 2 years in the same 18 recipients, and those with improved recovery parameters enjoyed a better HF-specific quality of life (P < 0.05 for both). In conclusion, parasympathetic reinnervation emerges along with improved post-exercise recovery ability of HR and quality of life during post-HTx 2 years.

  9. Humoral Hypercalcemia of Malignancy with a Parathyroid Hormone-Related Peptide-Secreting Intrahepatic Cholangiocarcinoma Accompanied by a Gastric Cancer

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    Katsushi Takeda

    2017-01-01

    Full Text Available Humoral hypercalcemia of malignancy (HHM is caused by the oversecretion of parathyroid hormone-related peptide (PTHrP from malignant tumors. Although any tumor may cause HHM, that induced by intrahepatic cholangiocarcinoma (ICC or gastric cancer (GC is rare. We report here a 74-year-old male who displayed HHM with both ICC and GC and showed an elevated serum PTHrP level. Treatment of the hypercalcemia with saline, furosemide, elcatonin, and zoledronic acid corrected his serum calcium level and improved symptoms. Because treatment of ICC should precede that of GC, we chose chemotherapy with cisplatin (CDDP and gemcitabine (GEM. Chemotherapy reduced the size of the ICC and decreased the serum PTHrP level. One year after diagnosis, the patient was alive in the face of a poor prognosis for an ICC that produced PTHrP. Immunohistochemical staining for PTHrP was positive for the ICC and negative for the GC, leading us to believe that the cause of the HHM was a PTHrP-secreting ICC. In conclusion, immunohistochemical staining for PTHrP may be useful in discovering the cause of HHM in the case of two cancers accompanied by an elevated serum PHTrP level. Chemotherapy with CDDP and GEM may be the most appropriate treatment for a PTHrP-secreting ICC.

  10. “El Sexo no es Malo”: Maternal Values Accompanying Contraceptive Use Advice to Young Latina Adolescent Daughters

    Science.gov (United States)

    Bravo, Magali; Cruz, Maria Elena; Rios, Rebeca M.; Kouyoumdjian, Claudia

    2010-01-01

    In this study, we utilized observational methods to identify maternal values and concerns accompanying contraceptive use advice in Latina mother–daughter sexuality conversations. The sample included non-sexually active early adolescents around 12 years of age and their mostly Spanish-speaking Latina mothers. Videotaped conversations were coded for the prevalence of messages related to four sexual values (abstinence, delay sex until older, sex is “normal”, sex is “improper”) and concerns about pregnancy and STD transmission. We examined whether the duration of time spent conversing about these messages was associated with participant characteristics, general communication openness, and the amount of time the dyads spent discussing contraceptive use. Results indicated that Latina mothers who had fewer years of education and lower family income talked longer to their daughters about the need to delay sex, avoid risky situations that would increase their chances of getting pregnant or acquiring an STD, and engage in self-protective practices. Less perceived openness in general communication as reported by both the mothers and the daughters was associated with increased time discussing that sex is improper. Although the duration of contraceptive use messages was brief, mothers and daughters who discussed the fact that sex is normal, and who communicated more about the importance of delaying sex, talked longer about contraceptive use practices compared to mothers and daughters who engaged in minimal discussion of these sexual values. PMID:20543876

  11. Effect of Leonurus cardiaca oil extract in patients with arterial hypertension accompanied by anxiety and sleep disorders.

    Science.gov (United States)

    Shikov, Alexander N; Pozharitskaya, Olga N; Makarov, Valery G; Demchenko, Dmitry V; Shikh, Evgenia V

    2011-04-01

    Leonurus cardiaca L. (Lamiaceae) is used traditionally for its sedative, hypotensive and cardiotonic effects. Due to the lack of clinical data regarding its effect in patients, a study was carried out to assess the clinical efficacy of Leonurus oil extract (LOE) in patients with arterial hypertension stages 1 and 2, accompanied by anxiety and sleep disorders. Fifty patients were treated for 28 days with 1200 mg LOE per day. Positive effects of LOE on psycho-emotional status and arterial blood pressure in patients with stage 1 hypertension were observed 1 week earlier than in patients with stage 2 hypertension. According to the Clinical Global Impression (CGI) scale, a significant improvement in the symptoms of anxiety and depression was observed in 32% of patients, a moderate improvement in 48% and a weak effect in 8%; 12% of patients did not respond to therapy. Side effects were minimal in all groups. Leonurus oil extract may therefore be a potentially effective therapeutic agent for patients with arterial hypertension and concurrent psycho-neurological disorders. Copyright © 2010 John Wiley & Sons, Ltd.

  12. Cryptococcal meningitis accompanying lymphocytic inflammation predominantly in cerebral deep white matter: a possible manifestation of immune reconstitution inflammatory syndrome.

    Science.gov (United States)

    Kuwahara, Hiroya; Tsuchiya, Kuniaki; Kobayashi, Zen; Inaba, Akira; Akiyama, Haruhiko; Mizusawa, Hidehiro

    2014-02-01

    Cryptococcal meningitis is rarely complicated by immune-mediated leukoencephalopathy, but the precise pathomechanism is uncertain. A 72-year-old Japanese man treated with prednisolone for Sweet disease developed a subacute progression of meningitis, which was considered as neuro-Sweet disease. A treatment by methylprednisolone rapidly improved CSF findings with a remarkable decrease in lymphocyte numbers in the blood, but the patient's consciousness still worsened after the cessation of the treatment. The patient developed cryptococcal meningitis and MRI showed abnormal intensities predominantly in the cerebral deep white matter along with the recovery of lymphocyte numbers in the blood, which resulted in death. A postmortem examination of the brain revealed degenerative lesions, especially at the cerebral white matter and cortex adjacent to the leptomeninges abundantly infiltrated by Cryptococcus neoformans. In the affected cerebral deep white matter, perivascular infiltration of lymphocytes was prominent in coexistence with reactive astrocytes and vascular proliferation, but these findings were not observed in the subcortical and cortical lesions. Cryptococcus neoformans was not present within the brain parenchyma. This is the first report of a case suggesting that cryptococcal meningitis can accompany lymphocytic inflammation predominantly in cerebral deep white matter as a possible manifestation of immune reconstitution inflammatory syndrome.

  13. Optical Effects Accompanying the Dynamical Bragg Diffraction in Linear 1D Photonic Crystals Based on Porous Silicon

    Directory of Open Access Journals (Sweden)

    Anton Maydykovskiy

    2014-10-01

    Full Text Available We survey our recent results on the observation and studies of the effects accompanying the dynamical Bragg diffraction in one-dimensional photonic crystals (PhC. Contrary to the kinematic Bragg diffraction, the dynamical one considers a continuous interaction between the waves travelling within a spatially-periodic structure and is the most pronounced in the so called Laue geometry, leading to a number of exciting phenomena. In the described experiments, we study the PhC based on porous silicon or porous quartz, made by the electrochemical etching of crystalline silicon with the consequent thermal annealing. Importantly, these PhC are approximately hundreds of microns thick and contain a few hundreds of periods, so that the experiments in the Laue diffraction scheme are available. We discuss the effect of the temporal splitting of femtosecond laser pulses and show that the effect is quite sensitive to the polarization and the phase of a femtosecond laser pulse. We also show the experimental realization of the Pendular effect in porous quartz PhC and demonstrate the experimental conditions for the total spatial switching of the output radiation between the transmitted and diffracted directions. All described effects are of high interest for the control over the light propagation based on PhC structures.

  14. Production of e+e- Pairs Accompanied by Nuclear Dissociation in Ultra-peripheral Heavy Ion Collisions

    Energy Technology Data Exchange (ETDEWEB)

    Adams, J.; Adler, C.; Aggarwal, M.M.; Ahammed, Z.; Allgower, C.; Amonett, J.; Anderson, B.D.; Anderson, M.; Arkhipkin, D.; Averichev, G.S.; Bai, Y.; Balewski, J.; Barannikova, O.; Barnby, L.S.; Baudot, J.; Bekele, S.; Belaga, V.V.; Bellwied, R.; Berger, J.; Bichsel, H.; Billmeier, A.; Bland, L.C.; Blyth, C.O.; Bonner, B.E.; Boucham, A.; Brandin, A.; Bravar, A.; Cadman, R.V.; Caines, H.; Calderon de la Barca Sanchez, M.; Cardenas, A.; Carroll, J.; Castillo, J.; Castro, M.; Cebra, D.; Chaloupka, P.; Chattopadhyay, S.; Chen, Y.; Chernenko, S.P.; Cherney, M.; Chikanian, A.; Choi, B.; Christie, W.; Coffin, J.P.; Cormier, T.M.; Corral, M.M.; Cramer, J.G.; Crawford, H.J.; Deng, W.S.; Derevschikov, A.A.; Didenko, L.; Dietel, T.; Draper, J.E.; Dunin, V.B.; Dunlop, J.C.; Eckardt, V.; Efimov, L.G.; Emelianov, V.; Engelage, J.; Eppley, G.; Erazmus, B.; Fachini, P.; Faine, V.; Faivre, J.; Fatemi, R.; Filimonov, K.; Finch, E.; Fisyak, Y.; Flierl, D.; Foley, K.J.; Fu, J.; Gagliardi, C.A.; Gagunashvili, N.; Gans, J.; Gaudichet, L.; Germain, M.; Geurts, F.; Ghazikhanian, V.; Grachov, O.; Grigoriev, V.; Guedon, M.; Guertin, S.M.; Gushin, E.; Hallman, T.J.; Hardtke, D.; Harris, J.W.; Heinz, M.; Henry, T.W.; Heppelmann, S.; Herston, T.; Hippolyte, B.; Hirsch, A.; Hjort, E.; Hoffmann, G.W.; Horsley, M.; Huang, H.Z.; Humanic, T.J.; Igo, G.; Ishihara, A.; Ivanshin, Yu.I.; Jacobs, P.; Jacobs, W.W.; Janik, M.; Johnson, I.; Jones, P.G.; Judd, E.G.; Kaneta, M.; Kaplan, M.; Keane, D.; Kiryluk, J.; Kisiel, A.; Klay, J.; Klein, S.R.; Klyachko, A.; Kollegger, T.; Konstantinov, A.; Kopytine, S.M.; Kotchenda, L.; Kovalenko, A.D.; Kramer, M.; Kravtsov, P.; Krueger, K.; Kuhn, C.; Kulikov, A.I.; Kunde, G.J.; Kunz, C.L.; Kutuev, R.K.; Kuznetsov, A.A.; Lamont, M.A.C.; Landgraf, J.M.; Lange, S.; Lansdell, C.P.; Lasiuk, B.; Laue, F.; Lauret, J.; Lebedev, A.; Lednicky, R.; Leontiev, V.M.; LeVine, M.J.; Li, Q.; Lindenbaum, S.J.; Lisa, M.A.; Liu, F.; Liu, L.; Liu, Z.; et al.

    2004-04-07

    We present the first data on e{sup +}e{sup -} pair production accompanied by nuclear breakup in ultra-peripheral gold-gold collisions at a center of mass energy of 200 GeV per nucleon pair. The nuclear breakup requirement selects events at small impact parameters, where higher-order corrections to the pair production cross section should be enhanced. We compare the pair kinematic distributions with two calculations: one based on the equivalent photon approximation, and the other using lowest-order quantum electrodynamics (QED); the latter includes the photon virtuality. The cross section, pair mass, rapidity and angular distributions are in good agreement with both calculations. The pair transverse momentum, p{sub T}, spectrum agrees with the QED calculation, but not with the equivalent photon approach. We set limits on higher-order contributions to the cross section. The e{sup +} and e{sup -} p{sub T} spectra are similar, with no evidence for interference effects due to higher-order diagrams.

  15. Nighttime mesospheric hydroxyl enhancements during SEP events and accompanying geomagnetic storms: Ionization rate modeling and Aura satellite observations

    Science.gov (United States)

    Verkhoglyadova, O. P.; Wissing, J. M.; Wang, S.; Kallenrode, M.-B.; Zank, G. P.

    2016-07-01

    We quantify the effects of combined precipitating solar protons and magnetospheric electrons on nighttime odd hydrogen density enhancements during two solar energetic particle (SEP) events accompanied by strong geomagnetic storms. We perform detailed modeling of ionization rates for 7-17 November 2004 and 20-30 August 2005 intervals with improved version 1.6 of the Atmospheric Ionization Module Osnabrück model. Particle measurements from Geostationary Operational Environmental Satellites and Polar Orbiting Environmental Satellites are sorted and combined in 2 h intervals to create realistic particle precipitation maps that are used as the modeling input. We show that modeled atmospheric ionization rates and estimated peak odd hydrogen (primarily hydroxyl) production from 0.001 hPa to 0.1 hPa atmospheric pressure levels during these intervals are consistent with enhancements in nighttime averaged zonal odd hydrogen densities derived from newly reprocessed and improved data set of Microwave Limb Sounder instrument on board Aura satellite. We show that both precipitating SEPs and magnetospheric electrons contribute to mesospheric ionization and their relative contributions change throughout the intervals. Our event-based modeling results underline the importance of the combined ionization sources for odd hydrogen chemistry in the middle atmosphere.

  16. Teaching Tacting of Private Events Based on Public Accompaniments: Effects of Contingencies, Audience Control, and Stimulus Complexity.

    Science.gov (United States)

    Stocco, Corey S; Thompson, Rachel H; Hart, John M

    2014-06-01

    Our current understanding of the role of private events in the science of behavior is based largely on Skinner's natural science interpretation of private events. Skinner described public accompaniments as one source of control for a verbal community to differentially reinforce verbal behavior regarding private events. In this study, we developed an experimental analogue to study variables influencing tacting of private events. The participant had exclusive access to one set of stimuli (the private stimuli), and the experimenter attempted to teach tacts for private stimuli based on their correspondence with public stimuli accessible to both the experimenter and participant. Results of experiments 1 and 2 demonstrated that reports of private stimuli were a function of degree of public-private correspondence, reinforcement contingency, and audience control. In some cases, we encountered reports controlled exclusively by public stimuli. Results of experiment 3 showed that public control was less likely when public stimuli were more complex and the experimenter had a unique behavioral history with respect to those stimuli that was not shared by the learner. The orderly patterns of data obtained suggest that analogue arrangements might be a useful, and even necessary, starting point for experimental investigations of how private events may enter into the analysis of behavior.

  17. Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats

    Directory of Open Access Journals (Sweden)

    Isaac A. Adedara

    2014-01-01

    Full Text Available 2,5-Hexanedione (2,5-HD is the toxic metabolite of n-hexane which is widely used as solvent in numerous industries. The present study elucidated the precise mechanism of 2,5-HD in hepatorenal toxicity by determining the involvement of oxidative stress in rats. Adult male Wistar rats were exposed to 0, 0.25, 0.5, and 1% 2,5-HD in drinking water for 21 days. Exposure to 2,5-HD caused liver and kidney atrophy evidenced by significant elevation in serum aminotransferases, alkaline phosphatase, albumin, bilirubin, urea, creatinine, and electrolytes levels compared with control. The marked dose-dependent increase in total cholesterol (TC, triglyceride (TG, and low-density lipoprotein (LDL was accompanied with significant decrease in high-density lipoprotein (HDL levels in 2,5-HD-exposed animals when compared with the control. Administration of 2,5-HD significantly diminished glutathione (GSH level but increased the activities of superoxide dismutase (SOD, catalase, glutathione peroxidase (GPx, and glutathione-S-transferase (GST concomitantly with marked elevation in hydrogen peroxide (H2O2 and malondialdehyde (MDA levels in liver and kidney of the treated groups compared with control. These findings suggest that undue exposure to 2,5-HD at environmentally relevant levels may impair liver and kidney functions through induction of oxidative stress.

  18. The supine-to-prone position change induces modification of endotracheal tube cuff pressure accompanied by tube displacement.

    Science.gov (United States)

    Minonishi, Toshiyuki; Kinoshita, Hiroyuki; Hirayama, Michiko; Kawahito, Shinji; Azma, Toshiharu; Hatakeyama, Noboru; Fujiwara, Yoshihiro

    2013-02-01

    To determine whether the supine-to-prone position change displaced the endotracheal tube (ETT) and, if so, whether the displacement related to this change modified ETT cuff pressure. Prospective study. Operating room of a university hospital. 132 intubated, adult, ASA physical status 1, 2, and 3 patients undergoing lumbar spine surgery. After induction of anesthesia, each patient's trachea was intubated. The insertion depth of each ETT was 23 cm for men and 21 cm for women at the upper incisors. In the supine position and after the supine-to-prone position change with the head rotated to the right, the length from the carina to ETT tip and ETT cuff pressure were measured. After the supine-to-prone position change, 91.7% patients had ETT tube displacement. Of these, 48% of patients' ETT moved ≥ 10 mm, whereas 86.3% of patients had changes in tube cuff pressure. There was a slight but significant correlation between ETT movement and change in cuff pressure. Depending on the position change, ETT cuff pressure decreased and the ETT tended to withdraw. After the supine-to-prone position change, patients had ETT tube displacement. Such ETT movement may be accompanied by a decrease in cuff pressure. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. Simple Syllabic Calls Accompany Discrete Behavior Patterns in Captive Pteronotus parnellii: An Illustration of the Motivation-Structure Hypothesis

    Directory of Open Access Journals (Sweden)

    Matthew J. Clement

    2012-01-01

    Full Text Available Mustached bats, Pteronotus parnellii, are highly social and vocal. Individuals of this species roost in tight clusters, and emit an acoustically rich repertoire of calls whose behavioral significance is largely unknown. We recorded their social and vocal behaviors within a colony housed under semi-natural conditions. We also quantified the spatial spread of each bat’s roosting location and discovered that this was relatively fixed and roughly confined to an individual’s body width. The spatial precision in roosting was accompanied by an equally remarkable match between specific vocalizations and well-timed, discrete, identifiable postures/behaviors, as revealed by logistic regression analysis. The bodily behaviors included crouching, marking, yawning, nipping, flicking, fighting, kissing, inspecting, and fly-bys. Two echolocation-like calls were used to maintain spacing in the colony, two noisy broadband calls were emitted during fights, two tonal calls conveyed fear, and another tonal call signaled appeasement. Overall, the results establish that mustached bats exhibit complex social interactions common to other social mammals. The correspondence of relatively low frequency and noisy, broadband calls with aggression, and of tonal, high frequency calls with fear supports Morton’s Motivation-Structure hypothesis, and establishes a link between motivation and the acoustic structure of social calls emitted by mustached bats.

  20. An accompanying genetic severe deficiency of tissue factor protects mice with a protein C deficiency from lethal endotoxemia.

    Science.gov (United States)

    Castellino, Francis J; Donahue, Deborah L; Navari, Rudolph M; Ploplis, Victoria A; Walsh, Mark

    2011-01-06

    Mice with a severe genetic deficiency of protein C (PC), PC(-/-)PC(tg4), display enhanced susceptibility to lethal effects of gram-negative endotoxemia induced by lipopolysaccharide (LPS), whereas mice severely deficient in tissue factor (TF), TF(-/-)hTF(tg), are protected from LPS-mediated lethality. In this study, we show that a simultaneous severe deficiency of TF protected low-PC mice from LPS-induced death, resulting in a survival profile similar to that experienced by wild-type (WT) mice. Plasma and whole blood coagulation assays, the latter measured by thromboelastography, demonstrated development of coagulopathies in LPS-treated mice, which were more severe in the case of the doubly deficient TF(-/-)hTF(tg)/PC(-/-)PC(tg4) mice, mainly reflecting earlier signs of disseminated intravascular coagulation in this latter cohort. Markers of inflammation were also elevated in response to LPS in both groups of mice at times just preceding death. We conclude that whereas coagulopathies are more exacerbated in LPS-treated TF(-/-)hTF(tg)/PC(-/-)PC(tg4) mice, the lowering of TF levels in mice with an accompanying severe PC deficiency confers protection against death compared with mice with a single severe PC deficiency. This suggests that proteases generated as a result of factor VIIa/TF-mediated thrombin generation play a mechanistic role in the enhanced lethality seen under very low PC conditions in an endotoxemia model in mice.