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Sample records for nci launches biorepository

  1. NCI Launches Proteomics Assay Portal | Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    In a paper recently published by the journal Nature Methods, Investigators from the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (NCI-CPTAC) announced the launch of a proteomics Assay Portal for multiple reaction monitoring-mass spectrometry (MRM-MS) assays.  This community web-based repository for well-characterized quantitative proteomic assays currently consists of 456 unique peptide assays to 282 unique proteins and ser

  2. Biorepositories- | Division of Cancer Prevention

    Science.gov (United States)

    Carefully collected and controlled high-quality human biospecimens, annotated with clinical data and properly consented for investigational use, are available through the Division of Cancer Prevention Biorepositories listed in the charts below. Biorepositories Managed by the Division of Cancer Prevention Biorepositories Supported by the Division of Cancer Prevention Related Biorepositories | Information about accessing biospecimens collected from DCP-supported clinical trials and projects.

  3. Biorepository for Selenium and Vitamin E Cancer Prevention Trial (SELECT) | Division of Cancer Prevention

    Science.gov (United States)

    As the largest prostate cancer prevention trial ever undertaken, the Selenium and Vitamin E Cancer Prevention Trial (SELECT) has assembled a substantial biorepository of specimens. To help make SELECT resources available to a wider research community, NCI and the Southwest Oncology Group are developing a plan for prostate cancer biology and nutritional science and micronutrient studies. |

  4. The Prostate Cancer Biorepository Network (PCBN)

    Science.gov (United States)

    2016-10-01

    1 Award Number: W81XWH-14-2-0183 TITLE: The Prostate Cancer Biorepository Network (PCBN) PRINCIPAL INVESTIGATOR: Colm Morrissey CONTRACTING... Cancer Biorepository Network (PCBN) 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-14-2-0183 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Colm Morrissey PhD...ABSTRACT The Genitourinary Cancer Biorepository at the University of Washington joined the Prostate Cancer Pathology Resource Network (PCBN) September

  5. Collaboration Opportunities with the Cancer Human Biobank (caHUB) at NCI | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The Biorepositories and Biospecimen Research Branch (BBRB) at the National Cancer Institute has developed the Cancer Human Biobank (caHUB), which is a unique infrastructure for collecting biospecimens for the purpose of conducting biospecimen research. Biospecimens from the BPV program will be made available to collaborators with the capability to perform molecular analysis as part of a collaborative research agreement with the NCI-BBRB.

  6. An NCI perspective on creating sustainable biospecimen resources.

    Science.gov (United States)

    Vaught, Jimmie; Rogers, Joyce; Myers, Kimberly; Lim, Mark David; Lockhart, Nicole; Moore, Helen; Sawyer, Sherilyn; Furman, Jeffrey L; Compton, Carolyn

    2011-01-01

    High-quality biospecimens with appropriate clinical annotation are critical in the era of personalized medicine. It is now widely recognized that biospecimen resources need to be developed and operated under established scientific, technical, business, and ethical/legal standards. To date, such standards have not been widely practiced, resulting in variable biospecimen quality that may compromise research efforts. The National Cancer Institute (NCI) Office of Biorepositories and Biospecimen Research (OBBR) was established in 2005 to coordinate NCI's biospecimen resource activities and address those issues that affect access to the high-quality specimens and data necessary for its research enterprises as well as the broader translational research field. OBBR and the NCI Biorepository Coordinating Committee developed NCI's "Best Practices for Biospecimen Resources" after consultation with a broad array of experts. A Biospecimen Research Network was established to fund research to develop additional evidence-based practices. Although these initiatives will improve the overall availability of high-quality specimens and data for cancer research, OBBR has been authorized to implement a national biobanking effort, cancer HUman Biobank (caHUB). caHUB will address systematically the gaps in knowledge needed to improve the state-of-the-science and strengthen the standards for human biobanking. This commentary outlines the progressive efforts by NCI in technical, governance, and economic considerations that will be important as the new caHUB enterprise is undertaken.

  7. NCI Designated Cancer Centers

    Science.gov (United States)

    ... Resources Conducting Clinical Trials Statistical Tools and Data Terminology Resources NCI Data Catalog Cryo-EM NCI's Role ... Report (RPPR) Grant Closeout Grant Resources NCI Grants Management Legal Requirements NCI Grant Policies Grants Management Contacts ...

  8. Establishment and operation of a biorepository for molecular epidemiologic studies in Costa Rica.

    Science.gov (United States)

    Cortés, Bernal; Schiffman, Mark; Herrero, Rolando; Hildesheim, Allan; Jiménez, Silvia; Shea, Katheryn; González, Paula; Porras, Carolina; Fallas, Greivin; Rodríguez, Ana Cecilia

    2010-04-01

    The Proyecto Epidemiológico Guanacaste (PEG) has conducted several large studies related to human papillomavirus (HPV) and cervical cancer in Guanacaste, Costa Rica in a long-standing collaboration with the U.S. National Cancer Institute. To improve molecular epidemiology efforts and save costs, we have gradually transferred technology to Costa Rica, culminating in state-of-the-art laboratories and a biorepository to support a phase III clinical trial investigating the efficacy of HPV 16/18 vaccine. Here, we describe the rationale and lessons learned in transferring molecular epidemiologic and biorepository technology to a developing country. At the outset of the PEG in the early 1990s, we shipped all specimens to repositories and laboratories in the United States, which created multiple problems. Since then, by intensive personal interactions between experts from the United States and Costa Rica, we have successfully transferred liquid-based cytology, HPV DNA testing and serology, chlamydia and gonorrhea testing, PCR-safe tissue processing, and viable cryopreservation. To accommodate the vaccine trial, a state-of-the-art repository opened in mid-2004. Approximately 15,000 to 50,000 samples are housed in the repository on any given day, and >500,000 specimens have been shipped, many using a custom-made dry shipper that permits exporting >20,000 specimens at a time. Quality control of shipments received by the NCI biorepository has revealed an error rate of <0.2%. Recently, the PEG repository has incorporated other activities; for example, large-scale aliquotting and long-term, cost-efficient storage of frozen specimens returned from the United States. Using Internet-based specimen tracking software has proven to be efficient even across borders. For long-standing collaborations, it makes sense to transfer the molecular epidemiology expertise toward the source of specimens. The successes of the PEG molecular epidemiology laboratories and biorepository prove that

  9. Emergency Preparedness at NCI

    Science.gov (United States)

    Information to help prepare for an emergency. Includes resources for patients and health care providers to continue cancer care, NCI contacts for grantees, and resources to prepare and update NCI employees and contractors.

  10. Design and utilization of the colorectal and pancreatic neoplasm virtual biorepository: An early detection research network initiative

    Directory of Open Access Journals (Sweden)

    Waqas Amin

    2010-01-01

    Full Text Available Background: The Early Detection Research Network (EDRN colorectal and pancreatic neoplasm virtual biorepository is a bioinformatics-driven system that provides high-quality clinicopathology-rich information for clinical biospecimens. This NCI-sponsored EDRN resource supports translational cancer research. The information model of this biorepository is based on three components: (a development of common data elements (CDE, (b a robust data entry tool and (c comprehensive data query tools. Methods: The aim of the EDRN initiative is to develop and sustain a virtual biorepository for support of translational research. High-quality biospecimens were accrued and annotated with pertinent clinical, epidemiologic, molecular and genomic information. A user-friendly annotation tool and query tool was developed for this purpose. The various components of this annotation tool include: CDEs are developed from the College of American Pathologists (CAP Cancer Checklists and North American Association of Central Cancer Registries (NAACR standards. The CDEs provides semantic and syntactic interoperability of the data sets by describing them in the form of metadata or data descriptor. The data entry tool is a portable and flexible Oracle-based data entry application, which is an easily mastered, web-based tool. The data query tool facilitates investigators to search deidentified information within the warehouse through a "point and click" interface thus enabling only the selected data elements to be essentially copied into a data mart using a dimensional-modeled structure from the warehouse′s relational structure. Results: The EDRN Colorectal and Pancreatic Neoplasm Virtual Biorepository database contains multimodal datasets that are available to investigators via a web-based query tool. At present, the database holds 2,405 cases and 2,068 tumor accessions. The data disclosure is strictly regulated by user′s authorization. The high-quality and well

  11. Anchor Trial Launch

    Science.gov (United States)

    NCI has launched a multicenter phase III clinical trial called the ANCHOR Study -- Anal Cancer HSIL (High-grade Squamous Intraepithelial Lesion) Outcomes Research Study -- to determine if treatment of HSIL in HIV-infected individuals can prevent anal canc

  12. NCI & Division Obligations

    Science.gov (United States)

    Displays obligations for grants, contracts, training fellowships, intramural research, and management and support, including the number of grant awards, funding amounts, and percent of the total NCI budget.

  13. At NCI, Supporting the Best Science

    Science.gov (United States)

    Yesterday, at the AACR annual meeting, Dr. Doug Lowy spoke directly to the research community about his goals as NCI Acting Director. Dr. Lowy said that he plans to continue many of the programs launched by his predecessor, Dr. Harold Varmus, and to sharp

  14. NCI launches smoking cessation support for teens

    Science.gov (United States)

    A new effort to help teens quit smoking will use one of today’s teen’s most constant companions—the mobile phone. Developed by smoking cessation experts, SmokefreeTXT is a free text message cessation service that provides 24/7 encouragement, advice, and

  15. NCI Contact Center

    Science.gov (United States)

    The NCI offers free, scientifically accurate, and easy-to-understand information on a range of cancer topics in English and Spanish. Get live help from compassionate information specialists at 1-800-4-CANCER.

  16. NCI at ASCO

    Science.gov (United States)

    NCI-designated cancer centers are being extensively represented at the annual meeting of the American Society for Clinical Oncology meeting in Chicago this year with a large array of clinical trial results and findings.

  17. NCI Visuals Online

    Science.gov (United States)

    NCI Visuals Online contains images from the collections of the National Cancer Institute's Office of Communications and Public Liaison, including general biomedical and science-related images, cancer-specific scientific and patient care-related images, and portraits of directors and staff of the National Cancer Institute.

  18. An Overview of Biorepositories-Past, Present, and Future.

    Science.gov (United States)

    Siwek, Martina

    2015-10-01

    The collection, storage, and distribution of biological materials for research and improving health have been employed for more than a century. Biorepositories have been used to maintain and reallocate these specimens. Historically, the Department of Defense (DoD) has been maintaining biorepositories and using the materials stored to expand our understanding of diseases and for developing medical countermeasures since the Civil War. Other U.S. Government and nongovernmental organizations are also engaged in curating human and other samples for future studies, as are organizations in other countries. The reasons for collection and the possible uses of specimens maintained within repositories have changed with the advent of novel technologies and the genomics discipline. However, over the years, many of the issues faced by repositories have remained largely the same, although of increased importance more recently because of limited funding and enhanced ethical concerns. These issues include what samples to collect; how to collect, transport, and store the samples; legal and ethical matters relating to sample collection and use; durability of analytes of interest in stored specimens; assessing the quality of stored specimens and providing researchers with statements of specimen quality; costs; maintenance and sustainability of the repository; and, implementing and maintaining laboratory quality programs and possibly accreditation. National and international scientific groups are working to identify and define best practices, but universal standards and practices remain challenges for the future. To begin addressing the above issues, the DoD implemented several initiatives, which are described elsewhere in this Military Medicine Supplement. Additionally, staff members working on the issues saw potential value in identifying other biorepository groups and similar work being done by these groups with the expectation of developing lines of communication and, eventually

  19. EDRN Longitudinal Serum Biorepository — EDRN Public Portal

    Science.gov (United States)

    Four percent of EDRN Registry mutation carriers developed cancer within a year after beginning follow-up in this registry; within 2 years, 10% had developed cancer. Within 2 years, 17% had developed either a cancer or a pre-malignant lesion (e.g. adenoma). Our goal is to recruit 265 mutation carriers from the EDRN High Risk Registry and Hereditary Cancer Center for the Longitudinal Serum Biorepository (LSB). Based on the cancer rates observed to date in the EDRN Registry, four years of follow-up in a group of this size would be expected to yield 53 invasive and in situ cancers and 37 benign premalignant lesions. All subjects in the serum biorepository (1)have signed consent (2) are counseled carriers (APC, BRCA1, BRCA2, CDH1, CDKN2A, MLH1, MSH2, MSH6) and (3) are enrolled in family studies through CU's Department of Preventive Medicine and/or are enrolled in the EDRN High Risk Registry. Post specimen medical history is collected annually. Serum and plasma are aliquoted for respository storage and stored in labeled cryovials in an ultra low temperature (-86C) freezer. Specimens in the LSB are available for use in EDRN biomarker development and validation studies.

  20. Data Sets from Major NCI Initiaves

    Science.gov (United States)

    The NCI Data Catalog includes links to data collections produced by major NCI initiatives and other widely used data sets, including animal models, human tumor cell lines, epidemiology data sets, genomics data sets from TCGA, TARGET, COSMIC, GSK, NCI60.

  1. Myositis registries and biorepositories: powerful tools to advance clinical, epidemiologic and pathogenic research

    Science.gov (United States)

    Rider, Lisa G.; Dankó, Katalin; Miller, Frederick W.

    2016-01-01

    Purpose of review Clinical registries and biorepositories have proven extremely useful in many studies of diseases, especially rare diseases. Given their rarity and diversity, the idiopathic inflammatory myopathies, or myositis syndromes, have benefited from individual researchers’ collections of cohorts of patients. Major efforts are being made to establish large registries and biorepositories that will allow many additional studies to be performed that were not possible before. Here we describe the registries developed by investigators and patient support groups that are currently available for collaborative research purposes. Recent findings We have identified 46 myositis research registries, including many with biorepositories, which have been developed for a wide variety of purposes and have resulted in great advances in understanding the range of phenotypes, clinical presentations, risk factors, pathogenic mechanisms, outcome assessment, therapeutic responses, and prognoses. These are now available for collaborative use to undertake additional studies. Two myositis patient registries have been developed for research, and myositis patient support groups maintain demographic registries with large numbers of patients available to be contacted for potential research participation. Summary Investigator-initiated myositis research registries and biorepositories have proven extremely useful in understanding many aspects of these rare and diverse autoimmune diseases. These registries and biorepositories, in addition to those developed by myositis patient support groups, deserve continued support to maintain the momentum in this field as they offer major opportunities to improve understanding of the pathogenesis and treatment of these diseases in cost-effective ways. PMID:25225838

  2. Sustainability in a Hospital-Based Biobank and University-Based DNA Biorepository: Strategic Roadmaps.

    Science.gov (United States)

    Seiler, Catherine Y; Eschbacher, Jennifer; Bowser, Robert; LaBaer, Joshua

    2015-12-01

    Sustainability in the biobanking community has recently become an important and oft-discussed issue as biorepositories struggle to balance limited external funding and complex cost recovery models with high operating costs and the desire to provide the highest quality materials and services to the research community. A multi-faceted view of biobanking sustainability requires consideration of operational and social sustainability in addition to the historical focus exclusively on financial sustainability. Planning and implementing this three pillar model creates a well-rounded biorepository that meets the needs of all the major stakeholders: the funders, the patients/depositors, and the researcher recipients. Often the creation of a detailed business plan is the first step to develop goals and objectives that lead down a path towards sustainability. The definition of sustainability and the complexity of a sustainable business plan may differ for each biorepository. The DNASU Plasmid Repository at Arizona State University stores and distributes DNA plasmids to researchers worldwide, and the Biobank Core Facility at St. Joseph's Hospital and Barrow Neurological Institute consents patients and collects, stores, and distributes human tissue and blood samples. We will discuss these two biorepositories, their similar and different approaches to sustainability and business planning, their challenges in creating and implementing their sustainability plan, and their responses to some of these challenges. From these experiences, the biobanks share lessons learned about planning for sustainability that are applicable to all biorepositories.

  3. NCI-Molecular Analysis for Therapy Choice (NCI-MATCH) Trial

    Science.gov (United States)

    NCI's gateway for information about the NCI-Molecular Analysis for Therapy Choice (NCI-MATCH) trial, in which patients with advanced cancer are assigned to treatment arms based on the molecular profiles of their disease.

  4. Comprehensive genomic studies: emerging regulatory, strategic, and quality assurance challenges for biorepositories.

    Science.gov (United States)

    McDonald, Sandra A; Mardis, Elaine R; Ota, David; Watson, Mark A; Pfeifer, John D; Green, Jonathan M

    2012-07-01

    As part of the molecular revolution sweeping medicine, comprehensive genomic studies are adding powerful dimensions to medical research. However, their power exposes new regulatory, strategic, and quality assurance challenges for biorepositories. A key issue is that unlike other research techniques commonly applied to banked specimens, nucleic acid sequencing, if sufficiently extensive, yields data that could identify a patient. This evolving paradigm renders the concepts of anonymized and anonymous specimens increasingly outdated. The challenges for biorepositories in this new era include refined consent processes and wording, selection and use of legacy specimens, quality assurance procedures, institutional documentation, data sharing, and interaction with institutional review boards. Given current trends, biorepositories should consider these issues now, even if they are not currently experiencing sample requests for genomic analysis. We summarize our current experiences and best practices at Washington University Medical School, St Louis, MO, our perceptions of emerging trends, and recommendations.

  5. NCI Alliance for Nanotechnology in Cancer

    Science.gov (United States)

    The NCI Alliance for Nanotechnology in Cancer funds the Cancer Nanotechnology Training Centers collectively with the NCI Cancer Training Center. Find out about the funded Centers, to date, that train our next generation of scientists in the field of Canc

  6. NCI collaborates with Multiple Myeloma Research Foundation

    Science.gov (United States)

    The National Cancer Institute (NCI) announced a collaboration with the Multiple Myeloma Research Foundation (MMRF) to incorporate MMRF's wealth of genomic and clinical data on the disease into the NCI Genomic Data Commons (GDC), a publicly available datab

  7. CRCHD Launches National Colorectal Cancer Outreach and Screening Initiative

    Science.gov (United States)

    The NCI CRCHD launches National Screen to Save Colorectal Cancer Outreach and Screening Initiative which aims to increase colorectal cancer screening rates among racially and ethnically diverse and rural communities.

  8. Generating a comprehensive set of standard operating procedures for a biorepository network-The CTRNet experience.

    Science.gov (United States)

    Barnes, Rebecca; Albert, Monique; Damaraju, Sambasivarao; de Sousa-Hitzler, Jean; Kodeeswaran, Sugy; Mes-Masson, Anne-Marie; Watson, Peter; Schacter, Brent

    2013-12-01

    Despite the integral role of biorepositories in fueling translational research and the advancement of medicine, there are significant gaps in harmonization of biobanking practices, resulting in variable biospecimen collection, storage, and processing. This significantly impacts accurate downstream analysis and, in particular, creates a problem for biorepository networks or consortia. The Canadian Tumour Repository Network (CTRNet; www.ctrnet.ca ) is a consortium of Canadian tumor biorepositories that aims to enhance biobanking capacity and quality through standardization. To minimize the issue of variable biobanking practices throughout its network, CTRNet has developed and maintained a comprehensive set of 45 standard operating procedures (SOPs). There were four key elements to the CTRNet SOP development process: 1) an SOP development team was formed from members across CTRNet to co-produce each SOP; 2) a principal author was appointed with responsibility for overall coordination of the SOP development process; 3) the CTRNet Management Committee (composed of principal investigators for each member biorepository) reviewed/revised each SOP completed by the development team; and 4) external expert reviewers provided feedback and recommendations on each SOP. Once final Management Committee approval was obtained, the ratified SOP was published on the CTRNet website for public access. Since the SOPs were first published on the CTRNet website (June 2008), there have been approximately 15,000 downloads of one or more CTRNet SOPs/Policies by users from over 60 countries. In accordance with biobanking best practices, CTRNet performs an exhaustive review of its SOPs at set intervals, to coincide with each granting cycle. The last revision was completed in May 2012.

  9. The use of Radio Frequency Identification to track samples in bio-repositories

    OpenAIRE

    GRIMSON, JANE BARCLAY

    2008-01-01

    PUBLISHED Bio-repositories are resources for storing biological samples and data to support the discovery of biomarkers, therapeutic targets, and the underlying causes of diseases. The success of this knowledge discovery process depends critically on the quality of samples and their associated data. Biological samples are expensive to collect and store. The samples and their associated data pass through a number of processes, generally in multiple locations, from data collection from the p...

  10. Progressive Consent and Specimen Accrual Models to Address Sustainability: A Decade's Experience at an Oregon Biorepository.

    Science.gov (United States)

    Ost, John A; Newton, Paul W; Neilson, Duncan R; Cioffi, Joseph A; Wackym, P Ashley; Perkins, R Serene

    2017-02-01

    The Legacy Biorepository is a College of American Pathologists-accredited biorepository operating within a seven-hospital healthcare system, with a decade's experience in specimen accrual, storage, and distribution. While standardization of our practices through accreditation remains a priority, we along with others face challenges with regard to sustainability. Purposeful changes in our consent process, which we term "progressive consent," are expected to improve sustainability and operational flexibility while increasing our scientific impact. Until 2015, informed consent was performed primarily by biorepository staff at an estimated time of 1 hour per case. After a process improvement exercise, we successfully changed our informed consent process to a modified front-door model, with use of material and data for research as an opt-in or opt-out selection on the institutional patient informed consent form provided to surgery patients in the healthcare system. Successful implementation of this change required the engagement and participation of multiple stakeholders in healthcare system leadership, hospital administration, research, legal, regulatory, and patient care levels. A modified front-door consent enabled us to collect an additional 38 specimens in the first two quarters of 2016, with a time commitment of 15.75 hours, a time savings per specimen increasing in Q2 over Q1. We estimate a potential savings of 43 hours in 2016. This progressive model allowed us to maintain our frozen sample collection while increasing the availability of paraffin-embedded tissue and bodily fluids. Augmenting our tissue collection added little expense per case (approximately half that of each frozen tissue aliquot) and increased the range of biospecimens collected. Biorepository financial sustainability is a critical issue. Thorough evaluation and modification of existing procedures and collection models, as well as cost recovery initiatives, can translate into savings

  11. Creating a global rare disease patient registry linked to a rare diseases biorepository database: Rare Disease-HUB (RD-HUB).

    Science.gov (United States)

    Rubinstein, Yaffa R; Groft, Stephen C; Bartek, Ronald; Brown, Kyle; Christensen, Ronald A; Collier, Elaine; Farber, Amy; Farmer, Jennifer; Ferguson, John H; Forrest, Christopher B; Lockhart, Nicole C; McCurdy, Kate R; Moore, Helen; Pollen, Geraldine B; Richesson, Rachel; Miller, Vanessa Rangel; Hull, Sara; Vaught, Jim

    2010-09-01

    A movement to create a global patient registry for as many as 7,000 rare diseases was launched at a workshop, "Advancing Rare Disease Research: The Intersection of Patient Registries, Biospecimen Repositories, and Clinical Data." http://rarediseases.info.nih.gov/PATIENT_REGISTRIES_WORKSHOP/. The workshop was sponsored by the Office of Rare Diseases Research (ORDR). The focus was the building of an infrastructure for an internet-based global registry linking to biorepositories. Such a registry would serve the patients, investigators, and drug companies. To aid researchers the participants suggested the creation of a centralized database of biorepositories for rare biospecimens (RD-HUB)http://biospecimens.ordr.info.nih.gov/ that could be linked to the registry. Over two days of presentations and breakout sessions, several hundred attendees discussed government rules and regulations concerning privacy and patients' rights and the nature and scope of data to be entered into a central registry as well as concerns about how to validate patient and clinician-entered data to ensure data accuracy. Mechanisms for aggregating data from existing registries were also discussed. The attendees identified registry best practices, model coding systems, international systems for recruiting patients into clinical trials and novel ways of using the internet directly to invite participation in research. They also speculated about who would bear ultimate responsibility for the informatics in the registry and who would have access to the information. Hurdles associated with biospecimen collection and how to overcome them were detailed. The development of the recommendations was, in itself, an indication of the commitment of the rare disease community as never before.

  12. Join TTC! | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The NCI Technology Transfer Center (TTC) offers a unique opportunity for training through the NCI TTC Fellowship program. TTC also has a unit dedicated to marketing these research opportunities and their underlying technologies to potential collaborators and licensees. | [google6f4cd5334ac394ab.html

  13. Trans-NCI Pharmacogenomics and Pharmacoepidemiology Working Group (PPWG)

    Science.gov (United States)

    NCI established the Trans-NCI Pharmacogenomics and Pharmacoepidemiology Working Group to support development of a comprehensive and interdisciplinary pharmacoepidemiology and pharmacogenomics cancer research program.

  14. Genomic Biorepository of Coastal Marine Species in Estero Padre Ramos and Estero Real, Nicaragua

    Directory of Open Access Journals (Sweden)

    Jorge A. Huete-Pérez

    2012-12-01

    Full Text Available Nicaragua, located in southern Mesoamerica between the Caribbean Sea and the Pacific Ocean, has acted as a land bridge for flora and fauna migrating between North and South America during the last 3 million years. Because of Nicaragua’s location and history, it is rich in terrestrial and aquatic biodiversity. To study this biodiversity and preserve it for the future, Nicaragua’s Molecular Biology Center at the University of Central America (CBM-UCA created the Genomic Biorepository Project. The Project collects and catalogs coastal marine biodiversity in the Estero Real and Padre Ramos estuaries, located in Nicaragua’s northern Pacific region.The biorepository holds more than three thousand tissue and genomic specimens, comprising 1,049 samples (714 specimens from Estero Padre Ramos and 335 from Estero Real belonging to 100 species and 54 families, genomic extracts in triplicates for every sample collected and environmental sandy sediments representing 60 different sites. Changes in the biological composition of the region were documented as compared to previous sampling. Of the 1,049 samples obtained from the two estuaries, 30 new residents were recorded in Estero Real, and 19 in Estero PadreRamos. The Cytochrome Oxidase I (COI gene was sequenced for a number of species, including 19 fish species, and published to public databases (BOLD SYSTEMS. The records contained in the genomic biorepository here described lay the foundation for the most complete marine biodiversity database in Nicaragua and is made available to national and international specialists, facilitating knowledge of Nicaraguan biodiversity.

  15. Biorepository standards and protocols for collecting, processing, and storing human tissues.

    Science.gov (United States)

    Troyer, Dean

    2008-01-01

    Recent advances in high-throughput assays for gene expression (genomics), proteins (proteomics), and metabolites (metabolomics) have engendered a parallel need for well-annotated human biological samples. Samples from both diseased and unaffected normal tissues are often required. Biorepositories consist of a specimen bank linked to a database of information. Assuring chain of custody and annotation of samples with relevant clinical information is required. The value of samples to end users is generally commensurate with the quality and extent of relevant clinical data included with the samples. Procurement of tissues is often done with parallel pre- and/or post-treatment venipuncture to obtain blood and tissue samples from the same subject. Biorepositories must also process, preserve, and distribute samples to end users. Like traditional libraries, biorepositories are meant to be used, and they are most useful when the needs of end users (researchers) are considered in the planning and development process. Ethics review and an awareness of regulatory requirements for storage, transport, and distribution are required. In the USA, Institutional Review Boards are the local regulatory entities that review protocols for banking of human biological tissues. Governmental and professional agencies and organizations provide some guidelines for standard operating procedures. The Food and Drug Administration (FDA), the Centers For Disease Control (CDC), and professional organizations such as the American Association of Tissue Banks (AATB), the American Association of Blood Banks, The International Red Cross, International Society for Biological Repositories (ISBER) and other organizations provide guidelines for biorepositories and banking of human tissues (see Table 1). To date, these guidelines are directed largely toward procurement, banking, and distribution of human tissues for therapeutic uses. In the international setting, the World Health Organization provides ethical

  16. Establishing and sustaining a biorepository network in Israel: challenges and progress.

    Science.gov (United States)

    Cohen, Yehudit; Almog, Ronit; Onn, Amir; Itzhaki-Alfia, Ayelet; Meir, Karen

    2013-12-01

    Over the past 5 years, using European and North American biobanks as models, the grass-roots establishment of independently operating biobanks has occurred virtually simultaneously in large Israeli teaching hospitals. The process of establishing a national biorepository network in Israel has progressed slowly, sustained mainly by a few proponents working together on a personal level. Slow progress has been due to limited funding and the lack of a legal framework specific to biobanking activities. Recently, due to increasing pressure from the scientific community, the government has earmarked funds for a national biorepository network, and the structure is now being established. In forming a network, Israel's biobanks face certain difficulties, particularly lack of support. Additional challenges include harmonization of standard operating procedures, database centralization, and use of a common informed consent form. In this article, we highlight some of the issues faced by Israel's biobank managers in establishing and sustaining a functional biobank network, information that could provide guidance for other small countries with limited resources.

  17. New directions in childhood obesity research: how a comprehensive biorepository will allow better prediction of outcomes

    Directory of Open Access Journals (Sweden)

    Woo Jessica G

    2010-10-01

    Full Text Available Abstract Background Childhood obesity is associated with the early development of diseases such as type 2 diabetes and cardiovascular disease. Unfortunately, to date, traditional methods of research have failed to identify effective prevention and treatment strategies, and large numbers of children and adolescents continue to be at high risk of developing weight-related disease. Aim To establish a unique 'biorepository' of data and biological samples from overweight and obese children, in order to investigate the complex 'gene × environment' interactions that govern disease risk. Methods The 'Childhood Overweight BioRepository of Australia' collects baseline environmental, clinical and anthropometric data, alongside storage of blood samples for genetic, metabolic and hormonal profiles. Opportunities for longitudinal data collection have also been incorporated into the study design. National and international harmonisation of data and sample collection will achieve required statistical power. Results Ethical approval in the parent site has been obtained and early data indicate a high response rate among eligible participants (71% with a high level of compliance for comprehensive data collection (range 56% to 97% for individual study components. Multi-site ethical approval is now underway. Conclusions In time, it is anticipated that this comprehensive approach to data collection will allow early identification of individuals most susceptible to disease, as well as facilitating refinement of prevention and treatment programs.

  18. NCI Central Review Board Receives Accreditation

    Science.gov (United States)

    The Association for the Accreditation of Human Research Protection Programs has awarded the NCI Central Institutional Review Board full accreditation. AAHRPP awards accreditation to organizations demonstrating the highest ethical standards in clinical res

  19. Dinutuximab (Unituxin™) | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    In 2010, NCI entered into a Cooperative Research and Development Agreement (CRADA) with United Therapeutics Corp., under which the company assumed responsibility for manufacturing dinutuximab and moving it through the steps required for regulatory approval.

  20. International Fellows of NCI at Frederick | Poster

    Science.gov (United States)

    Each year, the Employee Diversity Team (EDT) acknowledges members of the NCI at Frederick Community for their achievements and contributions towards the mission of facility.  Historically, the team has profiled the “Women of NCI at Frederick,” but this year, the team decided to instead shed light on the diverse and successful individuals who make up the international fellows community.

  1. International Fellows of NCI at Frederick | Poster

    Science.gov (United States)

    Each year, the Employee Diversity Team (EDT) acknowledges members of the NCI at Frederick Community for their achievements and contributions towards the mission of facility.  Historically, the team has profiled the “Women of NCI at Frederick,” but this year, the team decided to instead shed light on the diverse and successful individuals who make up the international fellows community.

  2. Overview and Utilization of the NCI Thesaurus

    Directory of Open Access Journals (Sweden)

    Larry Wright

    2006-04-01

    Full Text Available The NCI Thesaurus is a reference terminology covering areas of basic and clinical science, built with the goal of facilitating translational research in cancer. It contains nearly 110 000 terms in approximately 36000 concepts, partitioned in 20 subdomains, which include diseases, drugs, anatomy, genes, gene products, techniques, and biological processes, among others, all with a cancer-centric focus in content, and originally designed to support coding activities across the National Cancer Institute. Each concept represents a unit of meaning and contains a number of annotations, such as synonyms and preferred name, as well as annotations such as textual definitions and optional references to external authorities. In addition, concepts are modelled with description logic (DL and defined by their relationships to other concepts; there are currently approximately 90 types of named relations declared in the terminology. The NCI Thesaurus is produced by the Enterprise Vocabulary Services project, a collaborative effort between the NCI Center for Bioinformatics and the NCI Office of Communications, and is part of the caCORE infrastructure stack (http://ncicb.nci.nih.gov/NCICB/core. It can be accessed programmatically through the open caBIO API and browsed via the web (http://nciterms.nci.nih.gov. A history of editing changes is also accessible through the API. In addition, the Thesaurus is available for download in various file formats, including OWL, the web ontology language, to facilitate its utilization by others.

  3. NCI's Distributed Geospatial Data Server

    Science.gov (United States)

    Larraondo, P. R.; Evans, B. J. K.; Antony, J.

    2016-12-01

    Earth systems, environmental and geophysics datasets are an extremely valuable source of information about the state and evolution of the Earth. However, different disciplines and applications require this data to be post-processed in different ways before it can be used. For researchers experimenting with algorithms across large datasets or combining multiple data sets, the traditional approach to batch data processing and storing all the output for later analysis rapidly becomes unfeasible, and often requires additional work to publish for others to use. Recent developments on distributed computing using interactive access to significant cloud infrastructure opens the door for new ways of processing data on demand, hence alleviating the need for storage space for each individual copy of each product. The Australian National Computational Infrastructure (NCI) has developed a highly distributed geospatial data server which supports interactive processing of large geospatial data products, including satellite Earth Observation data and global model data, using flexible user-defined functions. This system dynamically and efficiently distributes the required computations among cloud nodes and thus provides a scalable analysis capability. In many cases this completely alleviates the need to preprocess and store the data as products. This system presents a standards-compliant interface, allowing ready accessibility for users of the data. Typical data wrangling problems such as handling different file formats and data types, or harmonising the coordinate projections or temporal and spatial resolutions, can now be handled automatically by this service. The geospatial data server exposes functionality for specifying how the data should be aggregated and transformed. The resulting products can be served using several standards such as the Open Geospatial Consortium's (OGC) Web Map Service (WMS) or Web Feature Service (WFS), Open Street Map tiles, or raw binary arrays under

  4. NCI at Frederick Employees Honored at NCI Director’s Awards Program | Poster

    Science.gov (United States)

    By Nancy Parrish, Staff Writer Nineteen staff members affiliated with NCI at Frederick or the Frederick National Laboratory for Cancer Research (FNLCR) were recognized at the 2014 NCI Director’s Award Ceremony for their outstanding contributions to advancing cancer research. The ceremony, held Dec. 1, took place at the NIH Natcher Conference Center, on the main campus in Bethesda.

  5. NCI at Frederick Employees Recognized at the 2013 NCI Director’s Awards Ceremony | Poster

    Science.gov (United States)

    By Andrea Frydl, Contributing Writer, and Ashley DeVine, Staff Writer More than 60 NCI at Frederick government and contractor employees were recognized at the NCI Director’s Awards Ceremony on Nov. 14, held on the main NIH campus in Bethesda.

  6. Towards a European Registry and Biorepository for Patients with Spinal and Bulbar Muscular Atrophy

    DEFF Research Database (Denmark)

    Pareyson, Davide; Fratta, Pietro; Pradat, Pierre-François

    2016-01-01

    Pathomechanisms of spinal and bulbar muscular atrophy (SBMA) have been extensively investigated and are partially understood, but no effective treatment is currently available for this disabling disorder. Its rarity, the slow disease progression, and lack of sensitive-to-change outcome measures...... as possible can be included, with the following aims: facilitate planning of clinical trials and recruitment of patients, define natural history of the disease, characterize epidemiology, develop standards of care, and inform the community of patients about research progresses and ongoing trials. We also aim...... at developing harmonized and coordinated biorepositories. The experience obtained during the last years in the field of other neuromuscular disorders and of Huntington disease offers valuable precedents....

  7. License Agreements | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    Since the government cannot engage in the development, manufacture, and sale of products, the NCI Technology Transfer Center (TTC) makes its discoveries (and discoveries from nine other NIH Institutes) available to organizations that can assist in the further development and commercialization of these basic science discoveries, to convert them into public health benefits. | [google6f4cd5334ac394ab.html

  8. NCI and Leidos Play Ball | Poster

    Science.gov (United States)

    By Carolynne Keenan, Contributing Writer The ping of an aluminum bat off a ball or the thump of a pop-up fly ball caught in a glove are two sounds familiar to baseball fans. Slow-pitch softball sounds—like those in the August game between mixed teams of NCI and Leidos Biomedical Research (formerly SAIC-Frederick) players—are similar.

  9. NCI International EBV-Gastric Cancer Consortium

    Science.gov (United States)

    A collaboration among NCI and extramural investigators, established by DCEG in 2006, that utilizes data and biospecimens from completed and ongoing case series and observational studies of gastric cancer to replicate and extend findings from previous studies hindered by small numbers of EBV-positive cases, and to stimulate multidisciplinary research in this area.

  10. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository.

    Science.gov (United States)

    Atherton, Daniel S; Sexton, Katherine C; Otali, Dennis; Bell, Walter C; Grizzle, William E

    2016-01-01

    The availability of high-quality human tissues is necessary to advance medical research. Although there are inherent and induced limitations on the use of human tissues in research, biorepositories play critical roles in minimizing the effects of such limitations. Specifically, the optimal utilization of tissues in research requires tissues to be diagnosed accurately, and the actual specimens provided to investigators must be carefully described (i.e., there must be quality control of each aliquot of the tissue provided for research, including a description of any damage to tissues). Tissues also should be collected, processed, stored, and distributed (i.e., handled) uniformly under a rigorous quality management system (QMS). Frequently, tissues are distributed to investigators by tissue banks which have collected, processed, and stored them by standard operating procedures (SOPs). Alternatively, tissues for research may be handled via SOPs that are modified to the specific requirements of investigators (i.e., using a prospective biorepository model). The primary goal of any type of biorepository should be to ensure its specimens are of high quality and are utilized appropriately in research; however, approaches may vary based on the tissues available and requested. For example, extraction of specific molecules (e.g., microRNA) to study molecular characteristics of a tissue may require less clinical annotation than tissues that are utilized to identify how the molecular expression might be used to clarify a clinical outcome of a disease or the response to a specific therapy. This review focuses on the limitations of the use of tissues in research and how the design and operations of a tissue biorepository can minimize some of these limitations.

  11. NCI at Frederick Ebola Response Team | Poster

    Science.gov (United States)

    Editor’s note: This article was adapted from the Employee Diversity Team’s display case exhibit “Recognizing the NCI at Frederick Ebola Response Team,” in the lobby of Building 549. The Poster staff recognizes that this article does not include everyone who was involved in the response to the Ebola crisis, both at NCI at Frederick and in Africa. When the Ebola crisis broke out in 2014 in West Africa, staff members from the Frederick National Laboratory for Cancer Research responded quickly. Members of the Clinical Monitoring Research Program (CMRP) were instrumental not only in setting up the clinical trials of the vaccine in Liberia, but also in providing training, community outreach, and recruitment strategies for the trials.

  12. UNC Cancer Center Director to Lead NCI.

    Science.gov (United States)

    2017-08-01

    President Donald Trump has selected Norman "Ned" Sharpless, MD, director of the University of North Carolina Lineberger Comprehensive Cancer Center, to lead the NCI. The news was met with widespread approval among cancer researchers, who view Sharpless as a strong communicator who can ably represent the needs of the cancer community in the face of proposed funding cuts. ©2017 American Association for Cancer Research.

  13. The NCI Delivery System for PDQ

    OpenAIRE

    1984-01-01

    The Physician Data Query System (PDQ) represents a major effort by the National Cancer Institute (NCI) to communicate advances in cancer treatment using computer technology. It utilizes a modern large scale computer mainframe to provide processing speed, a general purpose database management system to provide retrieval and display functions and flexibility, and commercial communications networks to provide access to an audience of physicians and other health care professionals seeking up-to-d...

  14. NCI's Transdisciplinary High Performance Scientific Data Platform

    Science.gov (United States)

    Evans, Ben; Antony, Joseph; Bastrakova, Irina; Car, Nicholas; Cox, Simon; Druken, Kelsey; Evans, Bradley; Fraser, Ryan; Ip, Alex; Kemp, Carina; King, Edward; Minchin, Stuart; Larraondo, Pablo; Pugh, Tim; Richards, Clare; Santana, Fabiana; Smillie, Jon; Trenham, Claire; Wang, Jingbo; Wyborn, Lesley

    2016-04-01

    The Australian National Computational Infrastructure (NCI) manages Earth Systems data collections sourced from several domains and organisations onto a single High Performance Data (HPD) Node to further Australia's national priority research and innovation agenda. The NCI HPD Node has rapidly established its value, currently managing over 10 PBytes of datasets from collections that span a wide range of disciplines including climate, weather, environment, geoscience, geophysics, water resources and social sciences. Importantly, in order to facilitate broad user uptake, maximise reuse and enable transdisciplinary access through software and standardised interfaces, the datasets, associated information systems and processes have been incorporated into the design and operation of a unified platform that NCI has called, the National Environmental Research Data Interoperability Platform (NERDIP). The key goal of the NERDIP is to regularise data access so that it is easily discoverable, interoperable for different domains and enabled for high performance methods. It adopts and implements international standards and data conventions, and promotes scientific integrity within a high performance computing and data analysis environment. NCI has established a rich and flexible computing environment to access to this data, through the NCI supercomputer; a private cloud that supports both domain focused virtual laboratories and in-common interactive analysis interfaces; as well as remotely through scalable data services. Data collections of this importance must be managed with careful consideration of both their current use and the needs of the end-communities, as well as its future potential use, such as transitioning to more advanced software and improved methods. It is therefore critical that the data platform is both well-managed and trusted for stable production use (including transparency and reproducibility), agile enough to incorporate new technological advances and

  15. Implementation of an error-reporting module within a biorepository IT application to enhance operations.

    Science.gov (United States)

    Wiles, Kerry R; Washington, M K

    2014-12-01

    The Collaborative (formerly the Cooperative) Human Tissue Network (CHTN) is a federally funded service oriented grant that provides high-quality biospecimens and services to the research community. The CHTN consists of six institutions located throughout the United States to assist investigators in obtaining research specimens required for basic research. The CHTN divisions have similar operating goals: however, each division is responsible for maintaining operations at their local institutions. This requires the divisions to identify ways to maintain and sustain operations in a challenging federally funded environment, especially when the number of investigators requesting services drives the operation. Sustainability plans and goals are often times patched together out of necessity rather than taking a thoughtful approach by clearly defining and aligning activities with business strategy and priorities. The CHTN Western Division at Vanderbilt University Medical Center (CHTN-WD) has responded to this challenge of biospecimen resource sustainability in the face of diminished funding by continually identifying ways to innovate our processes through IT enhancements and requiring that the innovation produce measurable and relevant criteria for credibly reporting our operations progress and performance issues. With these overarching goals in mind, CHTN-WD underwent a Lean Six Sigma (LSS) series to identify operational inefficiencies that could be addressed with redesigning workflow and innovating the processes using IT solutions. The result of this internal collaborative innovation process was the implementation of an error-reporting module (ERM) hosted within our biorepository donor IT application, which allowed staff to report errors immediately; determine the operational area responsible; assess the severity of the error; determine course of action; determine if standard operating procedure (SOPs) revisions were required; and through automated e-mails, alert the

  16. Implementation of an Error-Reporting Module Within a Biorepository IT Application to Enhance Operations

    Science.gov (United States)

    Washington, M.K.

    2014-01-01

    The Collaborative (formerly the Cooperative) Human Tissue Network (CHTN) is a federally funded service oriented grant that provides high-quality biospecimens and services to the research community. The CHTN consists of six institutions located throughout the United States to assist investigators in obtaining research specimens required for basic research. The CHTN divisions have similar operating goals: however, each division is responsible for maintaining operations at their local institutions. This requires the divisions to identify ways to maintain and sustain operations in a challenging federally funded environment, especially when the number of investigators requesting services drives the operation. Sustainability plans and goals are often times patched together out of necessity rather than taking a thoughtful approach by clearly defining and aligning activities with business strategy and priorities. The CHTN Western Division at Vanderbilt University Medical Center (CHTN-WD) has responded to this challenge of biospecimen resource sustainability in the face of diminished funding by continually identifying ways to innovate our processes through IT enhancements and requiring that the innovation produce measurable and relevant criteria for credibly reporting our operations progress and performance issues. With these overarching goals in mind, CHTN-WD underwent a Lean Six Sigma (LSS) series to identify operational inefficiencies that could be addressed with redesigning workflow and innovating the processes using IT solutions. The result of this internal collaborative innovation process was the implementation of an error-reporting module (ERM) hosted within our biorepository donor IT application, which allowed staff to report errors immediately; determine the operational area responsible; assess the severity of the error; determine course of action; determine if standard operating procedure (SOPs) revisions were required; and through automated e-mails, alert the

  17. NCI Community Oncology Research Program Approved | Division of Cancer Prevention

    Science.gov (United States)

    On June 24, 2013, the National Cancer Institute (NCI) Board of Scientific Advisors approved the creation of the NCI Community Oncology Research Program (NCORP). NCORP will bring state-of-the art cancer prevention, control, treatment and imaging clinical trials, cancer care delivery research, and disparities studies to individuals in their own communities. |

  18. Information for Our Partners | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    NCI TTC CRADA PAYMENT OPTIONS: Electronic Payments by Wire Transfer via Fedwire, Mail a check to the Institute or Center, or Automated Clearing House (ACH)/Electronic Funds Transfer (ETF) payments via Pay.gov (NCI ONLY). | [google6f4cd5334ac394ab.html

  19. Mission & Role | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The NCI TTC serves as the focal point for implementing the Federal Technology Transfer Act to utilize patents as incentive for commercial development of technologies and to establish research collaborations and licensing among academia, federal laboratories, non-profit organizations, and industry. The TTC supports technology development activities for the National Cancer Institute and nine other NIH Institutes and Centers. TTC staff negotiate co-development agreements and licenses with universities, non-profit organizations, and pharmaceutical and biotechnology companies to ensure compliance with Federal statutes, regulations and the policies of the National Institutes of Health. TTC also reviews employee invention reports and makes recommendations concerning filing of domestic and foreign patent applications. | [google6f4cd5334ac394ab.html

  20. Informatic system for a global tissue–fluid biorepository with a graph theory–oriented graphical user interface

    Directory of Open Access Journals (Sweden)

    William E. Butler

    2014-09-01

    Full Text Available The Richard Floor Biorepository supports collaborative studies of extracellular vesicles (EVs found in human fluids and tissue specimens. The current emphasis is on biomarkers for central nervous system neoplasms but its structure may serve as a template for collaborative EV translational studies in other fields. The informatic system provides specimen inventory tracking with bar codes assigned to specimens and containers and projects, is hosted on globalized cloud computing resources, and embeds a suite of shared documents, calendars, and video-conferencing features. Clinical data are recorded in relation to molecular EV attributes and may be tagged with terms drawn from a network of externally maintained ontologies thus offering expansion of the system as the field matures. We fashioned the graphical user interface (GUI around a web-based data visualization package. This system is now in an early stage of deployment, mainly focused on specimen tracking and clinical, laboratory, and imaging data capture in support of studies to optimize detection and analysis of brain tumour–specific mutations. It currently includes 4,392 specimens drawn from 611 subjects, the majority with brain tumours. As EV science evolves, we plan biorepository changes which may reflect multi-institutional collaborations, proteomic interfaces, additional biofluids, changes in operating procedures and kits for specimen handling, novel procedures for detection of tumour-specific EVs, and for RNA extraction and changes in the taxonomy of EVs. We have used an ontology-driven data model and web-based architecture with a graph theory–driven GUI to accommodate and stimulate the semantic web of EV science.

  1. Informatic system for a global tissue-fluid biorepository with a graph theory-oriented graphical user interface.

    Science.gov (United States)

    Butler, William E; Atai, Nadia; Carter, Bob; Hochberg, Fred

    2014-01-01

    The Richard Floor Biorepository supports collaborative studies of extracellular vesicles (EVs) found in human fluids and tissue specimens. The current emphasis is on biomarkers for central nervous system neoplasms but its structure may serve as a template for collaborative EV translational studies in other fields. The informatic system provides specimen inventory tracking with bar codes assigned to specimens and containers and projects, is hosted on globalized cloud computing resources, and embeds a suite of shared documents, calendars, and video-conferencing features. Clinical data are recorded in relation to molecular EV attributes and may be tagged with terms drawn from a network of externally maintained ontologies thus offering expansion of the system as the field matures. We fashioned the graphical user interface (GUI) around a web-based data visualization package. This system is now in an early stage of deployment, mainly focused on specimen tracking and clinical, laboratory, and imaging data capture in support of studies to optimize detection and analysis of brain tumour-specific mutations. It currently includes 4,392 specimens drawn from 611 subjects, the majority with brain tumours. As EV science evolves, we plan biorepository changes which may reflect multi-institutional collaborations, proteomic interfaces, additional biofluids, changes in operating procedures and kits for specimen handling, novel procedures for detection of tumour-specific EVs, and for RNA extraction and changes in the taxonomy of EVs. We have used an ontology-driven data model and web-based architecture with a graph theory-driven GUI to accommodate and stimulate the semantic web of EV science.

  2. NCI scientists at forefront of new prostate cancer diagnostics

    Science.gov (United States)

    Introduction of the UroNav was the result of nearly a decade’s research and development, principally conducted at NCI. Resembling a stylized computer workstation on wheels, the system electronically fuses together pictures from magnetic resonance imaging

  3. Micatu Tissue Arrayer | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    An NCI researcher recognized a critical need to create a low-cost, easy-to-use tissue microarrayer (TMA), an instrument used by researchers and pathologists to accurately examine tissue samples from patients.

  4. Brenda K. Edwards, PhD | DCCPS/NCI/NIH

    Science.gov (United States)

    Brenda K. Edwards, PhD, has been with the Surveillance Research Program (SRP) and its predecessor organizations at the National Cancer Institute (NCI) since 1989, serving as SRP’s Associate Director from 1990-2011.

  5. NCI at ASCO: A brief overview on women's cancers

    Science.gov (United States)

    The 2014 annual American Society of Clinical Oncology (ASCO) meeting in Chicago in June highlighted results from a number of NCI-supported and -sponsored clinical trial results in women’s cancers. Taken together, these results represent important advances

  6. Invention Development Program Helps Nurture NCI at Frederick Technologies | Poster

    Science.gov (United States)

    The Invention Development Fund (IDF) was piloted by the Technology Transfer Center (TTC) in 2014 to facilitate the commercial development of NCI technologies. The IDF received a second round of funding from the NCI Office of the Director and the Office of Budget and Management to establish the Invention Development Program (IDP) for fiscal year 2016. The IDP is using these funds to help advance a second set of inventions.

  7. Invention Development Program Helps Nurture NCI at Frederick Technologies | Poster

    Science.gov (United States)

    The Invention Development Fund (IDF) was piloted by the Technology Transfer Center (TTC) in 2014 to facilitate the commercial development of NCI technologies. The IDF received a second round of funding from the NCI Office of the Director and the Office of Budget and Management to establish the Invention Development Program (IDP) for fiscal year 2016. The IDP is using these funds to help advance a second set of inventions.

  8. Taiyuan Satellite Launch Center

    Institute of Scientific and Technical Information of China (English)

    LiuJie

    2004-01-01

    There are three major space launch bases in China, the Jiuquan Satellite Launch Center,the Taiyuan Satellite Launch Center and the Xichang Satellite Launch Center. All the three launch centers are located in sparsely populated areas where the terrain is even and the field of vision is broad. Security, transport conditions and the influence of the axial rotation

  9. SULFATE RESISTANCE MECHANISM OF HIGH-PERFORMANCE CONCRETE CONTAINING NCI

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    It is found that the incorporation of Nitrite Corrosion Inhibitor (NCI) greatly weakens the resistance of mixtures to sulfate attack.To study the mechanism of this phenomenon,in this paper,the influence of NCI additionon on the cement paste and microstructure change of high performance concrete specimens is studied by means of quantitative XRD,SEM tests.The results demonstrate that the incorporation of NCI accelerates the formation of calcium hydroxide and ettringite crystals,and weakens the pore refinement effect caused by the secondary hydration reaction of fly ash and microsilica.At the age up to one year,the relative crystal quantity in mixture containing NCI is always higher than that in control mixture.The reasons for the degradation in sulfate resisitance of mixtures may be attributed to the increase and stability of the calcium hydroxide and ettringite crystals formed and the weakening of secondary hydration reaction.Based on the results,conclusion can be drawn that NCI should be used cautiously in practical engineering where high resistance to sulfate attack is required.

  10. Xichang Satellite Launch Center

    Institute of Scientific and Technical Information of China (English)

    LiuJie

    2004-01-01

    Xichang Satellite Launch Center(XSLC) is mainly for geosynchronous orbit launches. The main purpose of XSLC is to launch spacecraft, such as broadcasting,communications and meteorological satellites, into geo-stationary orbit.Most of the commercial satellite launches of Long March vehicles have been from Xichang Satellite Launch Center. With 20 years' development,XSLC can launch 5 kinds of launch vehicles and send satellites into geostationary orbit and polar orbit. In the future, moon exploration satellites will also be launched from XSLC.

  11. The NCI Digital Divide Pilot Projects: implications for cancer education.

    Science.gov (United States)

    Kreps, Gary L; Gustafson, David; Salovey, Peter; Perocchia, Rosemarie Slevin; Wilbright, Wayne; Bright, Mary Anne; Muha, Cathy

    2007-01-01

    The National Cancer Institute (NCI) supported four innovative demonstration research projects, "The Digital Divide Pilot Projects," to test new strategies for disseminating health information via computer to vulnerable consumers. These projects involved active research collaborations between the NCI's Cancer Information Service (CIS) and regional cancer control researchers to field test new approaches for enhancing cancer communication in vulnerable communities. The projects were able to use computers to successfully disseminate relevant cancer information to vulnerable populations. These demonstration research projects suggested effective new strategies for using communication technologies to educate underserved populations about cancer prevention, control, and care.

  12. 75 FR 4827 - Submission for OMB Review; Comment Request Clinical Trials Reporting Program (CTRP) Database (NCI)

    Science.gov (United States)

    2010-01-29

    ... of the NCI's clinical trials portfolio, which is global in nature. The response further stated that... NCI to execute its mission to reduce the burden of cancer and to ensure an optimal return on the...

  13. The Employee Invention Report (EIR) | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    After making a unique, non-obvious, and useful discovery, NIH researchers must immediately contact their Laboratory or Branch Chief and inform him or her of a possible invention, and then consult with your NCI TTC Technology Transfer Manager about submitting an Employee Invention Report (EIR) Form. | [google6f4cd5334ac394ab.html

  14. NCI Community Oncology Research Program (NCORP) | Division of Cancer Prevention

    Science.gov (United States)

    The NCI Community Oncology Research Program (NCORP) is a national network of cancer care investigators, providers, academia, and other organizations that care for diverse populations in health systems. View the list of publications from NCORP. | Clinical Trials network of cancer care professionals who care for diverse populations across the U.S.

  15. Novel Method Of Preparing Vaccines | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    This invention from the NCI Cancer and Inflammation Program describes methods to prepare vaccines for the treatment of human immunodeficiency virus (HIV) infections. The National Cancer Institute's Cancer and Inflammation Program seeks parties interested in licensing or collaborative research to further develop, evaluate, or commercialize novel methods of preparing vaccines.

  16. NCI intramural research highlighted at 2014 AACR meeting

    Science.gov (United States)

    This year’s American Association for Cancer Research meeting featured plenary talks by two NCI scientists, Steven Rosenberg, M.D., and Louis Staudt, M.D., Ph.D., that highlighted the challenges in developing varied and potentially synergistic treatments f

  17. Russian delegation visits NIH and NCI to discuss research collaboration

    Science.gov (United States)

    The NCI Center for Global Health hosted a delegation from the Russian Foundation for Basic Research to discuss ongoing and future collaborations in cancer research. The delegation was accompanied by representatives from the US Embassy in Moscow and the Embassy of the Russian Federation in Washington DC.

  18. Cancer vaccines: the perspective of the Cancer Immunology Branch, NCI.

    Science.gov (United States)

    Sogn, J A; Finerty, J F; Heath, A K; Shen, G L; Austin, F C

    1993-08-12

    The Cancer Immunology Branch, NCI, is supporting a great deal of exciting research relevant to cancer vaccine development. The few areas highlighted here are representative of ongoing research opportunities, but further progress depends largely on a continued infusion of investigator-initiated ideas to realize the potential of current research areas and open new ones.

  19. 76 FR 14034 - Proposed Collection; Comment Request; NCI Cancer Genetics Services Directory Web-Based...

    Science.gov (United States)

    2011-03-15

    ... HUMAN SERVICES National Institutes of Health Proposed Collection; Comment Request; NCI Cancer Genetics Services Directory Web-Based Application Form and Update Mailer Summary: In compliance with the requirement... included in the NCI Cancer Genetics Services Directory on NCI's Cancer.gov Web site. The...

  20. Iraq Radiosonde Launch Records

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Iraqi upper air records loaned to NCDC from the Air Force 14th Weather Squadron. Scanned notebooks containing upper air radiosonde launch records and data. Launches...

  1. Launching technological innovations

    DEFF Research Database (Denmark)

    Talke, Katrin; Salomo, Søren

    2009-01-01

    have received less attention. This study considers the interdependencies between strategic, internally and externally, directed tactical launch activities and investigates both direct and indirect performance effects. The analysis is based upon data from 113 technological innovations launched...

  2. NCI Updates Tobacco Policies Following Re-accreditation | Poster

    Science.gov (United States)

    This year, NCI was re-accredited as one of nearly 200 CEO Cancer Gold Standard employers across the United States. According to its website, “the CEO Cancer Gold Standard provides a framework for employers to have a healthier workplace by focusing on cancer risk reduction, early detection, and access to clinical trials and high-quality care.” As part of this re-accreditation, NCI has updated its Tobacco-Free Policy. Part of this policy includes posting signs around campus reminding visitors and staff that NCI’s campus is tobacco-free. Therefore, the use of all tobacco products is prohibited. This includes cigarettes, cigars, pipes, e-cigarettes, and smokeless tobacco.

  3. Regular paths in SparQL: querying the NCI Thesaurus.

    Science.gov (United States)

    Detwiler, Landon T; Suciu, Dan; Brinkley, James F

    2008-11-06

    OWL, the Web Ontology Language, provides syntax and semantics for representing knowledge for the semantic web. Many of the constructs of OWL have a basis in the field of description logics. While the formal underpinnings of description logics have lead to a highly computable language, it has come at a cognitive cost. OWL ontologies are often unintuitive to readers lacking a strong logic background. In this work we describe GLEEN, a regular path expression library, which extends the RDF query language SparQL to support complex path expressions over OWL and other RDF-based ontologies. We illustrate the utility of GLEEN by showing how it can be used in a query-based approach to defining simpler, more intuitive views of OWL ontologies. In particular we show how relatively simple GLEEN-enhanced SparQL queries can create views of the OWL version of the NCI Thesaurus that match the views generated by the web-based NCI browser.

  4. NCI at Frederick Scientific Library Reintroduces Scientific Publications Database | Poster

    Science.gov (United States)

    A 20-year-old database of scientific publications by NCI at Frederick, FNLCR, and affiliated employees has gotten a significant facelift. Maintained by the Scientific Library, the redesigned database—which is linked from each of the Scientific Library’s web pages—offers features that were not available in previous versions, such as additional search limits and non-traditional metrics for scholarly and scientific publishing known as altmetrics.

  5. ``Thatcher's Ghost'': Confirmation of the ν Cygnids (NCY, IAU #409)

    Science.gov (United States)

    Jenniskens, Peter; Haberman, Bob

    2013-06-01

    A ghostly image of meteor radiants appeared in the CAMS data north-east of the Lyrids on April 22, 2012. The diffuse shower is identified as the ν Cygnids (NCY). It is weakly active from April 17 to 26, and likely related to the April ρ Cygnids (ARC), active in the area after April 27. The ν Cygnids may have been in outburst in 2012, because this shower was not as evident in the 2007-2011 SonotaCo data.

  6. NCI investment in nanotechnology: achievements and challenges for the future.

    Science.gov (United States)

    Dickherber, Anthony; Morris, Stephanie A; Grodzinski, Piotr

    2015-01-01

    Nanotechnology offers an exceptional and unique opportunity for developing a new generation of tools addressing persistent challenges to progress in cancer research and clinical care. The National Cancer Institute (NCI) recognizes this potential, which is why it invests roughly $150 M per year in nanobiotechnology training, research and development. By exploiting the various capacities of nanomaterials, the range of nanoscale vectors and probes potentially available suggests much is possible for precisely investigating, manipulating, and targeting the mechanisms of cancer across the full spectrum of research and clinical care. NCI has played a key role among federal R&D agencies in recognizing early the value of nanobiotechnology in medicine and committing to its development as well as providing training support for new investigators in the field. These investments have allowed many in the research community to pursue breakthrough capabilities that have already yielded broad benefits. Presented here is an overview of how NCI has made these investments with some consideration of how it will continue to work with this research community to pursue paradigm-changing innovations that offer relief from the burdens of cancer.

  7. Utilization of an EMR-biorepository to identify the genetic predictors of calcineurin-inhibitor toxicity in heart transplant recipients.

    Science.gov (United States)

    Oetjens, Matthew; Bush, William S; Birdwell, Kelly A; Dilks, Holli H; Bowton, Erica A; Denny, Joshua C; Wilke, Russell A; Roden, Dan M; Crawford, Dana C

    2014-01-01

    Calcineurin-inhibitors CI are immunosuppressive agents prescribed to patients after solid organ transplant to prevent rejection. Although these drugs have been transformative for allograft survival, long-term use is complicated by side effects including nephrotoxicity. Given the narrow therapeutic index of CI, therapeutic drug monitoring is used to prevent acute rejection from underdosing and acute toxicity from overdosing, but drug monitoring does not alleviate long-term side effects. Patients on calcineurin-inhibitors for long periods almost universally experience declines in renal function, and a subpopulation of transplant recipients ultimately develop chronic kidney disease that may progress to end stage renal disease attributable to calcineurin inhibitor toxicity (CNIT). Pharmacogenomics has the potential to identify patients who are at high risk for developing advanced chronic kidney disease caused by CNIT and providing them with existing alternate immunosuppressive therapy. In this study we utilized BioVU, Vanderbilt University Medical Center's DNA biorepository linked to de-identified electronic medical records to identify a cohort of 115 heart transplant recipients prescribed calcineurin-inhibitors to identify genetic risk factors for CNIT We identified 37 cases of nephrotoxicity in our cohort, defining nephrotoxicity as a monthly median estimated glomerular filtration rate (eGFR)pharmacogenomic genotyping platform that assays 184 variants across 34 genes. In Cox regression analysis adjusting for age at transplant, pre-transplant chronic kidney disease, pre-transplant diabetes, and the three most significant principal components (PCAs), we did not identify any markers that met our multiple-testing threshold. As a secondary analysis we also modeled post-transplant eGFR directly with linear mixed models adjusted for age at transplant, cyclosporine use, median BMI, and the three most significant principal components. While no SNPs met our threshold for

  8. NCI Scientists Awarded National Medal of Technology and Innovation by President Obama | Poster

    Science.gov (United States)

    Two NCI scientists received the National Medal of Technology and Innovation, the nation’s highest honor for technological achievement. The award was announced by President Obama in October. The honorees, John Schiller, Ph.D., Laboratory of Cellular Oncology (LCO), Center for Cancer Research, NCI, and Douglas Lowy, M.D., also from LCO and NCI deputy director, received their medals at a White House ceremony on Nov. 20.

  9. Prostate Cancer Biorepository Network

    Science.gov (United States)

    2016-10-01

    application to HHC through its research portal . This remains under committee review, however is expected to be resolved within the coming month...conference papers, and presentations Journal publications Books or other non-periodical, one-time publications. Other...Examples include original copies of journal articles, reprints of manuscripts and abstracts, a curriculum vitae, patent applications, study

  10. Vaccines for HIV | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The development of an effective HIV vaccine has been an ongoing area of research. The high variability in HIV-1 virus strains has represented a major challenge in successful development. Ideally, an effective candidate vaccine would provide protection against the majority of clades of HIV. Two major hurdles to overcome are immunodominance and sequence diversity. This vaccine utilizes a strategy for overcoming these two issues by identifying the conserved regions of the virus and exploiting them for use in a targeted therapy. NCI seeks licensees and/or research collaborators to commercialize this technology, which has been validated in macaque models.

  11. NCI at Frederick Employees Sew for Cancer | Poster

    Science.gov (United States)

    By Carolynne Keenan, Contributing Writer The R&W Club Frederick hosted a sewing party on Feb. 18 to give employees a chance to help sew pillowcases for children hospitalized for illnesses and cancer treatments. The nonprofit organization ConKerr Cancer provides the pillowcases to children across the country. Melissa Porter, administrative manager, Office of Scientific Operations, NCI at Frederick, and vice chair of the R&W Club Frederick, said the event went well. While the turnout was lower than expected, 27 pillowcases were completed, she said.

  12. New Phone System Coming to NCI Campus at Frederick | Poster

    Science.gov (United States)

    By Travis Fouche and Trent McKee, Guest Writers Beginning in September, phones at the NCI Campus at Frederick will begin to be replaced, as the project to upgrade the current phone system ramps up. Over the next 16 months, the Information Systems Program (ISP) will be working with Facilities Maintenance and Engineering and Computer & Statistical Services to replace the current Avaya phone system with a Cisco Unified Communications phone system. The Cisco system is already in use at the Advanced Technology Research Facility (ATRF).

  13. NCI Start-Up 2.0: An Evaluation Option License Program | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    NCI's Start-Up Evaluation Option License minimizes barriers to entry faced by start-up companies that seek to license NIH technologies. The license encourages and supports the commercial development of early-stage technologies developed in the NIH Intramural Research Program. While the NIH has been quite flexible in structuring licenses for the benefit of start-up companies, one of the goals of the NIH Start-up License program is to further reduce the time and capital to negotiate and finalize an exclusive license agreement. | [google6f4cd5334ac394ab.html

  14. Arianespace streamlines launch procedures

    Science.gov (United States)

    Lenorovitch, Jeffrey M.

    1992-06-01

    Ariane has entered a new operational phase in which launch procedures have been enhanced to reduce the length of launch campaigns, lower mission costs, and increase operational availability/flexibility of the three-stage vehicle. The V50 mission utilized the first vehicle from a 50-launcher production lot ordered by Arianespace, and was the initial flight with a stretched third stage that enhances Ariane's performance. New operational procedures were introduced gradually over more than a year, starting with the V42 launch in January 1991.

  15. 76 FR 22714 - Proposed Collection; Comment Request; Health Information National Trends Survey 4 (HINTS 4) (NCI)

    Science.gov (United States)

    2011-04-22

    ... understand vital cancer prevention messages. More importantly, this NCI survey will couple knowledge-related... National Trends Survey 4 (HINTS 4) (NCI) SUMMARY: In compliance with the requirement of Section 3506(c)(2... publish periodic summaries of proposed projects to be submitted to the Office of Management and Budget...

  16. Big Bang launch

    CERN Document Server

    2008-01-01

    Physicists from the University, along with scientists and engineers around the world, watched with fevered anticipation as the world's biggest scientific experiment was launched in September. (1/1 page)

  17. Genomic Data Commons launches

    Science.gov (United States)

    The Genomic Data Commons (GDC), a unified data system that promotes sharing of genomic and clinical data between researchers, launched today with a visit from Vice President Joe Biden to the operations center at the University of Chicago.

  18. IBF Launched in China

    Institute of Scientific and Technical Information of China (English)

    Guo Yan

    2009-01-01

    @@ The India Business Forum(IBF)organized by the Confederation of Indian Industry(CII)and the Indian Embassy to China was officially launched in Beijing,on April 16,2009.With the theme of"Impact of Global Economic Crisis:Challenges and Opportunities for India and China",IBF(China)was launched to provide a lobby to promote bilateral trade and economic cooperation between the two countries.

  19. New product launch

    OpenAIRE

    Andžič, Vedrana

    2007-01-01

    The aim of this Master's thesis is description of new electric water heater launch process. The theoretical part of the thesis deals with marketing mix and goes deeper in explanation of product lifecycle theory. Theoretical part is the basis for practical part. Practical part describes company Ariston Thermo in brief and deals with technical parameters of new electric water heater VELIS as well as distribution and price policy during the launch process. The key part of the thesis is dedicated...

  20. Evaluation of Product Launch

    OpenAIRE

    MARŠÁLKOVÁ, Nina

    2012-01-01

    This bachelor thesis deals with the evaluation of the launch of product on the market and the proposal of a more appropriate solution. Author has chosen company Aponia software, s.r.o. with a place of business in Brno. It is small company which produces and sells navigations for mobile devices. During writing this thesis author focus on the launch of navigation for operating system Android on the market.

  1. Softball Games Bring NCI and Leidos Biomed Employees Together | Poster

    Science.gov (United States)

    NCI and Leidos Biomed employees took to the fields at Nallin Pond for the third annual slow-pitch softball games on August 26. The series attracted 54 employees who were divided into four teams, Red, Blue, Gray, and White, and they were cheered on by about 40 enthusiastic spectators. In the first set of games, the Gray team defeated the Blue team, 15–8, and the White team pulled out a win against the Red team, 17–15. After a brief rest, the two winning teams and the two losing teams faced each other in a second set of games. On Field 1, the “winners” match-up of the Gray and White teams was a nail biter, with a close score throughout the game. Daylight was a factor, however, and the team captains decided to call the game for safety reasons. With a lead of 15 to 13, the Gray team was declared the overall winner.

  2. Softball Games Bring NCI and Leidos Biomed Employees Together | Poster

    Science.gov (United States)

    NCI and Leidos Biomed employees took to the fields at Nallin Pond for the third annual slow-pitch softball games on August 26. The series attracted 54 employees who were divided into four teams, Red, Blue, Gray, and White, and they were cheered on by about 40 enthusiastic spectators. In the first set of games, the Gray team defeated the Blue team, 15–8, and the White team pulled out a win against the Red team, 17–15. After a brief rest, the two winning teams and the two losing teams faced each other in a second set of games. On Field 1, the “winners” match-up of the Gray and White teams was a nail biter, with a close score throughout the game. Daylight was a factor, however, and the team captains decided to call the game for safety reasons. With a lead of 15 to 13, the Gray team was declared the overall winner.

  3. Persistent Identifier Practice for Big Data Management at NCI

    Directory of Open Access Journals (Sweden)

    Jingbo Wang

    2017-04-01

    Full Text Available The National Computational Infrastructure (NCI manages over 10 PB research data, which is co-located with the high performance computer (Raijin and an HPC class 3000 core OpenStack cloud system (Tenjin. In support of this integrated High Performance Computing/High Performance Data (HPC/HPD infrastructure, NCI’s data management practices includes building catalogues, DOI minting, data curation, data publishing, and data delivery through a variety of data services. The metadata catalogues, DOIs, THREDDS, and Vocabularies, all use different Uniform Resource Locator (URL styles. A Persistent IDentifier (PID service provides an important utility to manage URLs in a consistent, controlled and monitored manner to support the robustness of our national ‘Big Data’ infrastructure. In this paper we demonstrate NCI’s approach of utilising the NCI’s 'PID Service 'to consistently manage its persistent identifiers with various applications.

  4. Diretrizes nacionais para biorrepositório e biobanco de material biológico humano Brazilian guidelines for biorepositories and biobanks of human biological material

    Directory of Open Access Journals (Sweden)

    Gabriela Marodin

    2013-02-01

    Full Text Available OBJETIVO: Caracterizar a construção participativa e democrática das Diretrizes Nacionais para Biorrepositório e Biobanco de Material Biológico Humano com Finalidade de Pesquisa, baseada nos princípios éticos da dignidade humana, da autonomia, da beneficência, da justiça e da precaução. MÉTODOS: Para a elaboração do documento formou-se um grupo de trabalho interdisciplinar Bioética considerando os seguintes critérios: experiência na operacionalização de biobancos, Biobancos representatividade regional, tipo de material biológico acondicionado e especialistas em Biorrepositório bioética. Participaram, também, membros da Agência Nacional de Vigilância Sanitária Diretrizes - Anvisa, pela competência regulatória e da Comissão Nacional de Ética em Pesquisa - Conep, enquanto controle social. RESULTADOS: O documento, baseado nos preceitos éticos, legais e técnicos, apresenta os conceitos, as atividades, finalidades e diferenças entre biorrepositórios e biobancos, as formas de consentimento do sujeito, além de outros aspectos permeados pela preocupação do uso adequado da informação. As Diretrizes Nacionais para Biorrepositório e Biobanco de Material Biológico Humano com Finalidade de Pesquisa contém 39 artigos, dispostos em cinco capítulos. CONCLUSÃO: A importância de uma regulamentação surge da reflexão ética, considerando a moral, e tendo como norteador os aspectos legais, os quais se traduzem em um documento que não se esgota em si mesmo. A dinamicidade da ciência sempre nos remete à mudança de paradigmas, que podem ir além das legislações existentes.OBJECTIVE: To characterize the participatory and democratic creation of the Brazilian guidelines for biorepositories and biobanks of human biological material with the purpose of research based on the ethical principles of human dignity, autonomy, beneficence, justice, and precaution. METHODS: An interdisciplinary work group was constituted to

  5. VENESAT-1 Successfully Launched

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Venezuelan first satellite VENESAT-1 (or Simon Bolivar) was sent to space from the Xichang Satellite Launch Center(XSLC) at 0:53 (Beijing time) on October 30 atop a LM-3B launch vehicle. About 12 minutes later, the satellite entered the preset GTO orbit at the altitude of 36,000km. After four maneuvers, the satellite was normally positioned at 78 degrees west longitude at 15:39 (Beijing time) on November 9,beaming the majority of Latin America and part of the Caribbean region.

  6. A review of radio frequency identification technology for the anatomic pathology or biorepository laboratory: Much promise, some progress, and more work needed

    Directory of Open Access Journals (Sweden)

    Jerry J Lou

    2011-01-01

    Full Text Available Patient safety initiatives throughout the anatomic laboratory and in biorepository laboratories have mandated increasing emphasis on the need for accurately identifying and tracking biospecimen assets throughout their production lifecycle and for archiving/retrieval purposes. However, increasing production volume along with complex workflow characteristics, reliance on manual production processes, and required asset movement to disparate destinations throughout asset lifecycles continue to challenge laboratory efforts. Radio Frequency Identification (RFID technology, use of radio waves to communicate data between electronic tags attached to objects and a reader, shows significant potential to facilitate and overcome these hurdles. Advantages over traditional barcode labeling include readability without direct line-of-sight alignment to the reader, ability to read multiple tags simultaneously, higher data storage capacity, faster data transmission rate, and capacity to perform multiple read-writes of data to the tag. Most importantly, use of radio waves decreases the need to manually scan each asset, and at each step, identification or tracking event is needed. Temperature monitoring by on-board sensors and three-dimensional position tracking are additional potential benefits of using RFID technology. To date, barriers to implementation of RFID systems in the anatomic laboratory include increased associated costs of tags and readers, system software, data security concerns, lack of specific data standards for stored information, and potential for technological obsolescence during decades of specimen storage. Novel RFID production techniques and increased production capacity are projected to lower costs of some tags to a few cents each. Potentially, information security concerns can be addressed by techniques such as shielding, data encryption, and tag pseudonyms. Commitment by stakeholder groups to develop RFID tag data standards for anatomic

  7. A review of radio frequency identification technology for the anatomic pathology or biorepository laboratory: Much promise, some progress, and more work needed.

    Science.gov (United States)

    Lou, Jerry J; Andrechak, Gary; Riben, Michael; Yong, William H

    2011-01-01

    Patient safety initiatives throughout the anatomic laboratory and in biorepository laboratories have mandated increasing emphasis on the need for accurately identifying and tracking biospecimen assets throughout their production lifecycle and for archiving/retrieval purposes. However, increasing production volume along with complex workflow characteristics, reliance on manual production processes, and required asset movement to disparate destinations throughout asset lifecycles continue to challenge laboratory efforts. Radio Frequency Identification (RFID) technology, use of radio waves to communicate data between electronic tags attached to objects and a reader, shows significant potential to facilitate and overcome these hurdles. Advantages over traditional barcode labeling include readability without direct line-of-sight alignment to the reader, ability to read multiple tags simultaneously, higher data storage capacity, faster data transmission rate, and capacity to perform multiple read-writes of data to the tag. Most importantly, use of radio waves decreases the need to manually scan each asset, and at each step, identification or tracking event is needed. Temperature monitoring by on-board sensors and three-dimensional position tracking are additional potential benefits of using RFID technology. To date, barriers to implementation of RFID systems in the anatomic laboratory include increased associated costs of tags and readers, system software, data security concerns, lack of specific data standards for stored information, and potential for technological obsolescence during decades of specimen storage. Novel RFID production techniques and increased production capacity are projected to lower costs of some tags to a few cents each. Potentially, information security concerns can be addressed by techniques such as shielding, data encryption, and tag pseudonyms. Commitment by stakeholder groups to develop RFID tag data standards for anatomic pathology and

  8. Technology Refresh Program Launches Phase II | Poster

    Science.gov (United States)

    The Technology Refresh Program (TRP) is an NCI-funded initiative designed to promote efficient spending on computer equipment by providing staff members with access to the latest technology to meet their computing needs, said Kyle Miller, IT coordinator, Computer and Statistical Services (C&SS), NCI at Frederick.

  9. Athermal laser launch telescopes

    NARCIS (Netherlands)

    Kamphues, F.G.; Henselmans, R.; Rijnveld, N.; Lemmen, M.H.J.; Doelman, N.J.; Nijkerk, M.D.

    2011-01-01

    ESO has developed a concept for a compact laser guide star unit for use in future Adaptive Optics (AO) systems. A small powerful laser is combined with a telescope that launches the beam, creating a single modular unit that can be mounted directly on a large telescope. This approach solves several

  10. Athermal laser launch telescopes

    NARCIS (Netherlands)

    Kamphues, F.G.; Henselmans, R.; Rijnveld, N.; Lemmen, M.H.J.; Doelman, N.J.; Nijkerk, M.D.

    2011-01-01

    ESO has developed a concept for a compact laser guide star unit for use in future Adaptive Optics (AO) systems. A small powerful laser is combined with a telescope that launches the beam, creating a single modular unit that can be mounted directly on a large telescope. This approach solves several o

  11. Cassini launch contingency effort

    Science.gov (United States)

    Chang, Yale; O'Neil, John M.; McGrath, Brian E.; Heyler, Gene A.; Brenza, Pete T.

    2002-01-01

    On 15 October 1997 at 4:43 AM EDT, the Cassini spacecraft was successfully launched on a Titan IVB/Centaur on a mission to explore the Saturnian system. It carried three Radioisotope Thermoelectric Generators (RTGs) and 117 Light Weight Radioisotope Heater Units (LWRHUs). As part of the joint National Aeronautics and Space Administration (NASA)/U.S. Department of Energy (DoE) safety effort, a contingency plan was prepared to address the unlikely events of an accidental suborbital reentry or out-of-orbital reentry. The objective of the plan was to develop procedures to predict, within hours, the Earth impact footprints (EIFs) for the nuclear heat sources released during the atmospheric reentry. The footprint predictions would be used in subsequent notification and recovery efforts. As part of a multi-agency team, The Johns Hopkins University Applied Physics Laboratory (JHU/APL) had the responsibility to predict the EIFs of the heat sources after a reentry, given the heat sources' release conditions from the main spacecraft. (No ablation burn-through of the heat sources' aeroshells was expected, as a result of earlier testing.) JHU/APL's other role was to predict the time of reentry from a potential orbital decay. The tools used were a three degree-of-freedom trajectory code, a database of aerodynamic coefficients for the heat sources, secure links to obtain tracking data, and a high fidelity special perturbation orbit integrator code to predict time of spacecraft reentry from orbital decay. In the weeks and days prior to launch, all the codes and procedures were exercised. Notional EIFs were derived from hypothetical reentry conditions. EIFs predicted by JHU/APL were compared to those by JPL and US SPACECOM, and were found to be in good agreement. The reentry time from orbital decay for a booster rocket for the Russian Progress M-36 freighter, a cargo ship for the Mir space station, was predicted to within 5 minutes more than two hours before reentry. For the

  12. NCI Requests Targets for Monoclonal Antibody Production and Characterization - Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    In an effort to provide well-characterized monoclonal antibodies to the scientific community, NCI's Antibody Characterization Program requests cancer-related protein targets for affinity production and distribution.

  13. Identification of a new restriction endonuclease R.NciII, from Neisseria cinerea.

    Science.gov (United States)

    Piekarowicz, A

    1994-01-01

    Site-specific restriction endonuclease R. Nci II has been purified from Neisseria cinerea strain 32615. The enzyme recognizes the sequence 5' GATC 3' and its activity is inhibited by the presence of methylated adenine residue within the recognition sequence.

  14. NCI and the Chinese Academy of Medical Sciences Sign Statement of Intent

    Science.gov (United States)

    Today the National Cancer Institute (NCI) and the Cancer Institute/Hospital of the Chinese Academy of Medical Sciences (CICAMS) signed a statement of intent to share an interest in fostering collaborative biomedical research in oncology and a common goal

  15. National Medal of Technology Awarded to NCI Drs. Lowy and Schiller

    Science.gov (United States)

    President Obama announced that two NCI scientists would be recipients of the National Medal of Technology and Innovation -- the nation's highest honor for technological achievement. The honorees, John Schiller, Ph.D., Laboratory of Cellular Oncology (LCO)

  16. Gardasil® and Cervarix® | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    Vaccine for human papilloma virus (HPV) to protect from cancers Key elements of the technology for Gardasil® and Cervarix originated from the HPV research of the laboratory of Drs. Douglas Lowy and John Schiller of the NCI.

  17. Ratio Based Biomarkers for the Prediction of Cancer Survival | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The NCI seeks licensees or co-development partners for this technology, which describes compositions, methods and kits for identifying, characterizing biomolecules expressed in a sample that are associated with the presence, the development, or progression of cancer.

  18. College Graduate with NCI Internship Gains Experience, Carries Chemistry into Medicine | Poster

    Science.gov (United States)

    For Jennifer Marshall, the skills learned through an internship at the National Cancer Institute (NCI) at Frederick have prepared her for the next step of her life—medical school. Marshall, who will be attending the West Virginia University School of Medicine in the fall, spent three summers in NCI at Frederick’s Summer Internship Program expanding her love and passion for science and the medical field.

  19. Estadística descriptiva per a ciències de la salut

    OpenAIRE

    Sanjuan i Nebot, Lluís Francesc

    2016-01-01

    Aquests materials han rebut un incentiu del Servei de Política Lingüística Apunts d'estadística descriptiva per a estudients de l'àrea de les ciències de la salut Apunts d'estadística descriptiva orientats a graus de l'àrea de les ciències de la salut

  20. Estadística descriptiva per a ciències de la salut

    OpenAIRE

    Sanjuan i Nebot, Lluís Francesc

    2016-01-01

    Aquests materials han rebut un incentiu del Servei de Política Lingüística Apunts d'estadística descriptiva per a estudients de l'àrea de les ciències de la salut Apunts d'estadística descriptiva orientats a graus de l'àrea de les ciències de la salut

  1. STS-121 Launch

    Science.gov (United States)

    2006-01-01

    Space Shuttle Discovery and its seven-member crew launched at 2:38 p.m. (EDT) to begin the two-day journey to the International Space Station (ISS) on the historic Return to Flight STS-121 mission. The shuttle made history as it was the first human-occupying spacecraft to launch on Independence Day. During its 12-day mission, this utilization and logistics flight delivered a multipurpose logistics module (MPLM) to the ISS with several thousand pounds of new supplies and experiments. In addition, some new orbital replacement units (ORUs) were delivered and stowed externally on the ISS on a special pallet. These ORUs are spares for critical machinery located on the outside of the ISS. During this mission the crew also carried out testing of Shuttle inspection and repair hardware, as well as evaluated operational techniques and concepts for conducting on-orbit inspection and repair.

  2. Data mining the NCI60 to predict generalized cytotoxicity.

    Science.gov (United States)

    Lee, Adam C; Shedden, Kerby; Rosania, Gustavo R; Crippen, Gordon M

    2008-07-01

    Elimination of cytotoxic compounds in the early and later stages of drug discovery can help reduce the costs of research and development. Through the application of principal components analysis (PCA), we were able to data mine and prove that approximately 89% of the total log GI 50 variance is due to the nonspecific cytotoxic nature of substances. Furthermore, PCA led to the identification of groups of structurally unrelated substances showing very specific toxicity profiles, such as a set of 45 substances toxic only to the Leukemia_SR cancer cell line. In an effort to predict nonspecific cytotoxicity on the basis of the mean log GI 50, we created a decision tree using MACCS keys that can correctly classify over 83% of the substances as cytotoxic/noncytotoxic in silico, on the basis of the cutoff of mean log GI 50 = -5.0. Finally, we have established a linear model using least-squares in which nine of the 59 available NCI60 cancer cell lines can be used to predict the mean log GI 50. The model has R (2) = 0.99 and a root-mean-square deviation between the observed and calculated mean log GI 50 (RMSE) = 0.09. Our predictive models can be applied to flag generally cytotoxic molecules in virtual and real chemical libraries, thus saving time and effort.

  3. Launch of Zoological Letters.

    Science.gov (United States)

    Fukatsu, Takema; Kuratani, Shigeru

    2016-02-01

    A new open-access journal, Zoological Letters, was launched as a sister journal to Zoological Science, in January 2015. The new journal aims at publishing topical papers of high quality from a wide range of basic zoological research fields. This review highlights the notable reviews and research articles that have been published in the first year of Zoological Letters, providing an overview on the current achievements and future directions of the journal.

  4. Launching Another Crisis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    North Korea's test-firing of a range of missiles gets a mixed reaction from the international communityWhen the Democratic People's Republic of Korea (DPRK) test-fired a stream of missiles on July 5, it drew sharp concerns from a global communi- ty perpetually concerned over stability and security in the Asia-Pacific region. According to government reports from South Korea, the DPRK launched at least seven missiles from two sites along its east

  5. Launch Control Network Engineer

    Science.gov (United States)

    Medeiros, Samantha

    2017-01-01

    The Spaceport Command and Control System (SCCS) is being built at the Kennedy Space Center in order to successfully launch NASA’s revolutionary vehicle that allows humans to explore further into space than ever before. During my internship, I worked with the Network, Firewall, and Hardware teams that are all contributing to the huge SCCS network project effort. I learned the SCCS network design and the several concepts that are running in the background. I also updated and designed documentation for physical networks that are part of SCCS. This includes being able to assist and build physical installations as well as configurations. I worked with the network design for vehicle telemetry interfaces to the Launch Control System (LCS); this allows the interface to interact with other systems at other NASA locations. This network design includes the Space Launch System (SLS), Interim Cryogenic Propulsion Stage (ICPS), and the Orion Multipurpose Crew Vehicle (MPCV). I worked on the network design and implementation in the Customer Avionics Interface Development and Analysis (CAIDA) lab.

  6. Launch Services Program EMC Issues

    Science.gov (United States)

    trout, Dawn

    2004-01-01

    Presentation covers these issues: (1) Vehicles of the Launch Services Program, (2) RF Environment, (3) Common EMC Launch Vehicle Payload Integration Issues, (4) RF Sensitive Missions and (5) Lightning Monitoring,

  7. Experimental Satellite 2 Successfully Launched

    Institute of Scientific and Technical Information of China (English)

    LiuJie

    2004-01-01

    Small satellite Experimental Satellite 2 (SY-2) was launched by LM-2C launch vehicle from Xichang Satellite Launch Center on Nov. 18, 2004. Later the satellite entered the preset sun-synchronous orbit, which is 700 kilometers above the earth. The launch was the eighthmission this year by China Aerospace Science and Technology Corporation(CASC), which aims to test the technology of the satellite, conduct survey and monitoring of the land and resources and geographical environment on a trial basis.

  8. Launch Stabilisation System for Vertical Launch of a Missile

    Directory of Open Access Journals (Sweden)

    K. Sreekumar

    2005-07-01

    Full Text Available The launch platform stabilisation control system is a roll-pitch stabilised platform for the vertical launch of a missile from a naval ship. Stabilisation of the launch platform is achievedwith the help of embedded controllers and electro-hydraulic servo control system. The launch platform is stabilised wrt true horizontal with a 2-axis (roll and pitch stabilisation systemconsisting of a gimbal and a set of three high-pressure servo hydraulic actuators. The control system uses rate gyro and tilt sensor feedbacks for stabilising the platform. This paper outlines the details of the launch platform stabilisation control system, results of digital simulation, and the performance during sea trials.

  9. Expendable launch vehicle studies

    Science.gov (United States)

    Bainum, Peter M.; Reiss, Robert

    1995-01-01

    Analytical support studies of expendable launch vehicles concentrate on the stability of the dynamics during launch especially during or near the region of maximum dynamic pressure. The in-plane dynamic equations of a generic launch vehicle with multiple flexible bending and fuel sloshing modes are developed and linearized. The information from LeRC about the grids, masses, and modes is incorporated into the model. The eigenvalues of the plant are analyzed for several modeling factors: utilizing diagonal mass matrix, uniform beam assumption, inclusion of aerodynamics, and the interaction between the aerodynamics and the flexible bending motion. Preliminary PID, LQR, and LQG control designs with sensor and actuator dynamics for this system and simulations are also conducted. The initial analysis for comparison of PD (proportional-derivative) and full state feedback LQR Linear quadratic regulator) shows that the split weighted LQR controller has better performance than that of the PD. In order to meet both the performance and robustness requirements, the H(sub infinity) robust controller for the expendable launch vehicle is developed. The simulation indicates that both the performance and robustness of the H(sub infinity) controller are better than that for the PID and LQG controllers. The modelling and analysis support studies team has continued development of methodology, using eigensensitivity analysis, to solve three classes of discrete eigenvalue equations. In the first class, the matrix elements are non-linear functions of the eigenvector. All non-linear periodic motion can be cast in this form. Here the eigenvector is comprised of the coefficients of complete basis functions spanning the response space and the eigenvalue is the frequency. The second class of eigenvalue problems studied is the quadratic eigenvalue problem. Solutions for linear viscously damped structures or viscoelastic structures can be reduced to this form. Particular attention is paid to

  10. CHINA LAUNCHES NEW SCIENTIFIC SATELLITE

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    China on Sept. 27, 2004 launched a scientific satellite atop a Long March 2D carrier rocket from Jiuquan Satellite Launch Center in Gansu province. 10 minutes after the launch, the satellite entered a preset orbit and is running sound at the orbit. It is the 20th recoverable satellite for scientific and technological

  11. Launch Vehicle Control Center Architectures

    Science.gov (United States)

    Watson, Michael D.; Epps, Amy; Woodruff, Van; Vachon, Michael Jacob; Monreal, Julio; Levesque, Marl; Williams, Randall; Mclaughlin, Tom

    2014-01-01

    Launch vehicles within the international community vary greatly in their configuration and processing. Each launch site has a unique processing flow based on the specific launch vehicle configuration. Launch and flight operations are managed through a set of control centers associated with each launch site. Each launch site has a control center for launch operations; however flight operations support varies from being co-located with the launch site to being shared with the space vehicle control center. There is also a nuance of some having an engineering support center which may be co-located with either the launch or flight control center, or in a separate geographical location altogether. A survey of control center architectures is presented for various launch vehicles including the NASA Space Launch System (SLS), United Launch Alliance (ULA) Atlas V and Delta IV, and the European Space Agency (ESA) Ariane 5. Each of these control center architectures shares some similarities in basic structure while differences in functional distribution also exist. The driving functions which lead to these factors are considered and a model of control center architectures is proposed which supports these commonalities and variations.

  12. New Product Launching Ideas

    Science.gov (United States)

    Kiruthika, E.

    2012-09-01

    Launching a new product can be a tense time for a small or large business. There are those moments when you wonder if all of the work done to develop the product will pay off in revenue, but there are many things are can do to help increase the likelihood of a successful product launch. An open-minded consumer-oriented approach is imperative in todayís diverse global marketplace so a firm can identify and serve its target market, minimize dissatisfaction, and stay ahead of competitors. Final consumers purchase for personal, family, or household use. Finally, the kind of information that the marketing team needs to provide customers in different buying situations. In high-involvement decisions, the marketer needs to provide a good deal of information about the positive consequences of buying. The sales force may need to stress the important attributes of the product, the advantages compared with the competition; and maybe even encourage ìtrialî or ìsamplingî of the product in the hope of securing the sale. The final stage is the post-purchase evaluation of the decision. It is common for customers to experience concerns after making a purchase decision. This arises from a concept that is known as ìcognitive dissonance

  13. Magnetic Launch Assist

    Science.gov (United States)

    Jacobs, W. A.

    2000-01-01

    With the ever-increasing cost of getting to space and the need for safe, reliable, and inexpensive ways to access space, NASA is taking a look at technologies that will get us there. One of these technologies is Magnetic Launch Assist (MagLev). This is the concept of using both magnetic levitation and magnetic propulsion to provide an initial velocity by using electrical power from ground sources. The use of ground based power can significantly reduce operational costs over the consumables necessary to attain the same velocity. The technologies to accomplish this are both old and new. The concept of MagLev has been around for a long time and several MagLev Trains have already been made. Where NASA's MagLev diverges from the traditional train is in the immense power required to propel this vehicle to 600 feet per second in less than 10 seconds. New technologies or the upgrade of existing technologies will need to be investigated in areas of energy storage and power switching. Plus the separation of a very large mass (the space vehicle) and the aerodynamics of that vehicle while on the carrier are also of great concern and require considerable study and testing. NASA's plan is to mature these technologies in the next 10 years to achieve our goal of launching a full sized space vehicle off a MagLev rail.

  14. Launch Decisions of Pharmaceutical Companies

    Directory of Open Access Journals (Sweden)

    Abdülkadir Civan

    2016-04-01

    Full Text Available This paper models the launch decision of pharmaceutical companies in regard to new drugs and country markets. New drugs are launched with a delay or not launched at all in many countries. Considering that many of these new drugs would have created health benefits to the patients, there seems to be welfare loss. We use market characteristics to explain this phenomenon. We show that most of the estimated launch with a delay and no-launch decision is due to observable market characteristics. The model has an accuracy of 70 percent in explaining the no-launch decision. Intellectual property rights protection is especially important. The policy implication is that stronger property rights increase the likelihood and speed of new drug launch.

  15. 14 CFR 417.111 - Launch plans.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Launch plans. 417.111 Section 417.111... TRANSPORTATION LICENSING LAUNCH SAFETY Launch Safety Responsibilities § 417.111 Launch plans. (a) General. A launch operator must implement written launch plans that define how launch processing and flight of a...

  16. Launch vehicle selection model

    Science.gov (United States)

    Montoya, Alex J.

    1990-01-01

    Over the next 50 years, humans will be heading for the Moon and Mars to build scientific bases to gain further knowledge about the universe and to develop rewarding space activities. These large scale projects will last many years and will require large amounts of mass to be delivered to Low Earth Orbit (LEO). It will take a great deal of planning to complete these missions in an efficient manner. The planning of a future Heavy Lift Launch Vehicle (HLLV) will significantly impact the overall multi-year launching cost for the vehicle fleet depending upon when the HLLV will be ready for use. It is desirable to develop a model in which many trade studies can be performed. In one sample multi-year space program analysis, the total launch vehicle cost of implementing the program reduced from 50 percent to 25 percent. This indicates how critical it is to reduce space logistics costs. A linear programming model has been developed to answer such questions. The model is now in its second phase of development, and this paper will address the capabilities of the model and its intended uses. The main emphasis over the past year was to make the model user friendly and to incorporate additional realistic constraints that are difficult to represent mathematically. We have developed a methodology in which the user has to be knowledgeable about the mission model and the requirements of the payloads. We have found a representation that will cut down the solution space of the problem by inserting some preliminary tests to eliminate some infeasible vehicle solutions. The paper will address the handling of these additional constraints and the methodology for incorporating new costing information utilizing learning curve theory. The paper will review several test cases that will explore the preferred vehicle characteristics and the preferred period of construction, i.e., within the next decade, or in the first decade of the next century. Finally, the paper will explore the interaction

  17. Pharmacologically directed strategies in academic anticancer drug discovery based on the European NCI compounds initiative.

    Science.gov (United States)

    Hendriks, Hans R; Govaerts, Anne-Sophie; Fichtner, Iduna; Burtles, Sally; Westwell, Andrew D; Peters, Godefridus J

    2017-07-11

    The European NCI compounds programme, a joint initiative of the EORTC Research Branch, Cancer Research Campaign and the US National Cancer Institute, was initiated in 1993. The objective was to help the NCI in reducing the backlog of in vivo testing of potential anticancer compounds, synthesised in Europe that emerged from the NCI in vitro 60-cell screen. Over a period of more than twenty years the EORTC-Cancer Research Campaign panel reviewed ∼2000 compounds of which 95 were selected for further evaluation. Selected compounds were stepwise developed with clear go/no go decision points using a pharmacologically directed programme. This approach eliminated quickly compounds with unsuitable pharmacological properties. A few compounds went into Phase I clinical evaluation. The lessons learned and many of the principles outlined in the paper can easily be applied to current and future drug discovery and development programmes. Changes in the review panel, restrictions regarding numbers and types of compounds tested in the NCI in vitro screen and the appearance of targeted agents led to the discontinuation of the European NCI programme in 2017 and its transformation into an academic platform of excellence for anticancer drug discovery and development within the EORTC-PAMM group. This group remains open for advice and collaboration with interested parties in the field of cancer pharmacology.

  18. Annotation and query of tissue microarray data using the NCI Thesaurus

    Directory of Open Access Journals (Sweden)

    Montgomery Kelli

    2007-08-01

    Full Text Available Abstract Background The Stanford Tissue Microarray Database (TMAD is a repository of data serving a consortium of pathologists and biomedical researchers. The tissue samples in TMAD are annotated with multiple free-text fields, specifying the pathological diagnoses for each sample. These text annotations are not structured according to any ontology, making future integration of this resource with other biological and clinical data difficult. Results We developed methods to map these annotations to the NCI thesaurus. Using the NCI-T we can effectively represent annotations for about 86% of the samples. We demonstrate how this mapping enables ontology driven integration and querying of tissue microarray data. We have deployed the mapping and ontology driven querying tools at the TMAD site for general use. Conclusion We have demonstrated that we can effectively map the diagnosis-related terms describing a sample in TMAD to the NCI-T. The NCI thesaurus terms have a wide coverage and provide terms for about 86% of the samples. In our opinion the NCI thesaurus can facilitate integration of this resource with other biological data.

  19. LHCb launches new website

    CERN Multimedia

    2008-01-01

    A new public website for the LHCb experiment was launched last Friday to coincide with CERN’s Open Day weekend. Designed to provide accessible information on all aspects of the experiment, the website contains images and key facts about the LHCb detector, its design and installation and the international team behind the project. "LHCb is going to be one of the most important b-physics experiments in the world when it starts taking data later this year", explains Roger Forty, the experiment’s deputy spokesperson. "We hope the website will be a valuable resource, enabling people to learn about this fascinating area of research." The new website can be found at: http://cern.ch/lhcb-public

  20. Innovacions amb blogs: desenvolupament de competències del futur mestre i benestar subjectiu docent

    Directory of Open Access Journals (Sweden)

    Mª Rosario Neira Piñeiro

    2012-12-01

    Full Text Available Es descriuen tres experiències innovadores del Grau de Maestro amb blogs, orientades al desenvolupament de competències específiques i transversals: un blog de ressenyes literàries infantils; una revista digital com a espai per a la investigació i el treball col·laboratiu; i un i-portafolio per visibilizar pràctiques, avaluar progressos en l'aprenentatge i desenvolupar tutories. Es va constatar en totes elles una alta participació, motivació i implicació, i el desenvolupament de competències d'ordre superior, com les habilitats comunicatives, digitals, d'anàlisis i síntesis…. Amb això, el professorat va experimentar un alt grau de benestar subjectiu, vinculat amb la seva satisfacció en relació a les innovacions realitzades.

  1. Cytotoxicity against KB and NCI-H187 cell lines of modified flavonoids from Kaempferia parviflora.

    Science.gov (United States)

    Yenjai, Chavi; Wanich, Suchana

    2010-05-01

    Flavones 1-4 isolated from Kaempferia parviflora were used for structural modification. Sixteen flavonoid derivatives, including four new derivatives, were synthesized and evaluated for cytotoxicity against KB and NCI-H187 cell lines. Flavanones 2a-4a demonstrated higher cytotoxic activity than the parent compounds. Cytotoxicity against KB cell line of oxime 1c was about 7 times higher than the ellipticine standard. Interestingly, oximes 1c and 2c exhibited highly potent cytotoxicity against NCI-H187 cell line with IC(50) values of 0.014 and 0.23 microM, respectively. Oximes 4c and 5c showed strong cytotoxicity against NCI-H187 cell line with IC(50) values of 4.04 and 2.32 microM, respectively. 2010 Elsevier Ltd. All rights reserved.

  2. LM-3B Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    RenShufang

    2005-01-01

    LM-3B launch vehicle is a heavy three-stage liquid propellant strap-on launch vehicle, which was developed based on the mature technologies of the LM-3A and LM-2E. It not only has the highest payload capacity to send China's satellites to GTO, but is also one of the most advanced launch vehicles in the world with high reliability, reasonable price and perfect technological design.

  3. Peer Review of Launch Environments

    Science.gov (United States)

    Wilson, Timmy R.

    2011-01-01

    Catastrophic failures of launch vehicles during launch and ascent are currently modeled using equivalent trinitrotoluene (TNT) estimates. This approach tends to over-predict the blast effect with subsequent impact to launch vehicle and crew escape requirements. Bangham Engineering, located in Huntsville, Alabama, assembled a less-conservative model based on historical failure and test data coupled with physical models and estimates. This white paper summarizes NESC's peer review of the Bangham analytical work completed to date.

  4. EHS and FME Lend Their Expertise to NCI Campus Refurbishment Project | Poster

    Science.gov (United States)

    In October 2015, the NCI executive officer and the director of NCI’s Office of Space and Facilities Management (OSFM) announced a wide-ranging refurbishment plan for NCI at Frederick. Since then, a project team comprising members from the Office of Scientific Operations, the Management Operations Support Branch, OSFM, the Center for Cancer Research, the Environment, Health, and Safety (EHS) directorate, and the Facilities Maintenance and Engineering (FME) directorate have met regularly with the laboratory groups affected by the refurbishment plan. Read more...

  5. Diagnostic Marker for Improving Treatment Outcomes of Hepatitis C | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    NCI Researchers have discovered Interferon-lambda 4 (IFNL4), a protein found through analysis of genomic data. Preliminary studies indicate that this protein may play a role in the clearance of HCV and may be a new target for diagnosing and treating HCV infection. The National Cancer Institute (NCI) Division of Cancer Epidemiology and Genetics (DCEG) Immunoepidemiology Branch is seeking statements of capability or interest from parties interested in in-licensing or collaborative research to further co-develop a gene-based diagnostic for Hepatitis C virus (HepC, HCV).

  6. Connecting Genomic Alterations to Cancer Biology with Proteomics: The NCI Clinical Proteomic Tumor Analysis Consortium

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, Matthew; Gillette, Michael; Carr, Steven A.; Paulovich, Amanda G.; Smith, Richard D.; Rodland, Karin D.; Townsend, Reid; Kinsinger, Christopher; Mesri, Mehdi; Rodriguez, Henry; Liebler, Daniel

    2013-10-03

    The National Cancer Institute (NCI) Clinical Proteomic Tumor Analysis Consortium is applying the latest generation of proteomic technologies to genomically annotated tumors from The Cancer Genome Atlas (TCGA) program, a joint initiative of the NCI and the National Human Genome Research Institute. By providing a fully integrated accounting of DNA, RNA, and protein abnormalities in individual tumors, these datasets will illuminate the complex relationship between genomic abnormalities and cancer phenotypes, thus producing biologic insights as well as a wave of novel candidate biomarkers and therapeutic targets amenable to verifi cation using targeted mass spectrometry methods.

  7. LM-4B Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    RenShufang

    2004-01-01

    The history of LM-4B traces back to the end of the 1970s. The feasibility study of LM-4 began in 1982 and the engineering development was initiated in the following year.Initially, the LM-4 served as a back-up launch vehicle for LM-3 to launch China's communications satellites. After the successful launch of China's first communications satellites by LM-3 in 1984, the main mission of the LM-4 was shifted to launch sun-synchronous orbit meteorological satellites.

  8. Urban poor program launched.

    Science.gov (United States)

    1991-01-01

    The government of the Philippines has launched a program to deal with the rapidly growing urban poor population. 60 cities (including Metro Manila) are expected to increase their bloated population by 3.8% over 1990 which would be 27.7 million for 1991. Currently there is an exodus of people from the rural areas and by 2000 half the urban population will be squatters and slum dwellers. Basic services like health and nutrition are not expected to be able to handle this type of volume without a loss in the quality of service. The basic strategy of the new program is to recruit private medical practitioners to fortify the health care delivery and nutrition services. Currently the doctor/urban dweller ration is 1:9000. The program will develop a system to pool the efforts of government and private physicians in servicing the target population. Barangay Escopa has been chosen as the pilot city because it typifies the conditions of a highly populated urban area. The projects has 2 objectives: 1) demonstrate the systematic delivery of health and nutrition services by the private sector through the coordination of the government, 2) reduce mortality and morbidity in the community, especially in the 0-6 age group as well as pregnant women and lactating mothers.

  9. Launch Support Video Site

    Science.gov (United States)

    OFarrell, Zachary L.

    2013-01-01

    The goal of this project is to create a website that displays video, countdown clock, and event times to customers during launches, without needing to be connected to the internal operations network. The requirements of this project are to also minimize the delay in the clock and events to be less than two seconds. The two parts of this are the webpage, which will display the data and videos to the user, and a server to send clock and event data to the webpage. The webpage is written in HTML with CSS and JavaScript. The JavaScript is responsible for connecting to the server, receiving new clock data, and updating the webpage. JavaScript is used for this because it can send custom HTTP requests from the webpage, and provides the ability to update parts of the webpage without having to refresh the entire page. The server application will act as a relay between the operations network, and the open internet. On the operations network side, the application receives multicast packets that contain countdown clock and events data. It will then parse the data into current countdown times and events, and create a packet with that information that can be sent to webpages. The other part will accept HTTP requests from the webpage, and respond to them with current data. The server is written in C# with some C++ files used to define the structure of data packets. The videos for the webpage will be shown in an embedded player from UStream.

  10. AMS ready for launch

    CERN Multimedia

    Katarina Anthony

    2011-01-01

    On 29 April, the Alpha Magnetic Spectrometer (AMS) will complete its long expedition to the International Space Station on board the space shuttle Endeavour. The Endeavour is set to lift off from NASA’s Kennedy Space Station at 15:47 EST (21:47 CET).   Samuel Ting, principal investigator for the AMS project, and Rolf Heuer, CERN Director-General, visit the Kennedy Space Centre before the AMS launch.  Courtesy of NASA and Kennedy Space Center. AMS is a CERN recognised experiment, created by an internal collaboration of 56 institutes. It will be the first large magnetic spectrometer to be used in space, and has been designed to function as an external module on the ISS. AMS will measure cosmic rays without atmospheric interference, allowing researchers on the ground to continue their search for dark matter and antimatter in the Universe. Data collected by AMS will be analysed in CERN’s new AMS Control Centre in Building 946 (due for completion in June 2011). The End...

  11. 76 FR 28439 - Submission for OMB Review; Comment Request; NCI Cancer Genetics Services Directory Web-Based...

    Science.gov (United States)

    2011-05-17

    ... HUMAN SERVICES National Institutes of Health Submission for OMB Review; Comment Request; NCI Cancer Genetics Services Directory Web-Based Application Form and Update Mailer Summary: Under the provisions of... Collection: Title: NCI Cancer Genetics Services Directory Web-based Application Form and Update Mailer....

  12. LM-3A Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    RenShufang

    2004-01-01

    The LM-3A launch vehicle is a large three-stage liquidpropellant launch vehicle developed on the basis ot LM-3 ana LM-2C. By incorporating the mature technologies of LM-3 and adding a more powerful improved LOX/LH cryogenic third stage and more capable control system, LM-3A has a

  13. Launch Vehicle Dynamics Demonstrator Model

    Science.gov (United States)

    1963-01-01

    Launch Vehicle Dynamics Demonstrator Model. The effect of vibration on launch vehicle dynamics was studied. Conditions included three modes of instability. The film includes close up views of the simulator fuel tank with and without stability control. [Entire movie available on DVD from CASI as Doc ID 20070030984. Contact help@sti.nasa.gov

  14. Small Space Launch: Origins & Challenges

    Science.gov (United States)

    Freeman, T.; Delarosa, J.

    2010-09-01

    The United States Space Situational Awareness capability continues to be a key element in obtaining and maintaining the high ground in space. Space Situational Awareness satellites are critical enablers for integrated air, ground and sea operations, and play an essential role in fighting and winning conflicts. The United States leads the world space community in spacecraft payload systems from the component level into spacecraft, and in the development of constellations of spacecraft. In the area of launch systems that support Space Situational Awareness, despite the recent development of small launch vehicles, the United States launch capability is dominated by an old, unresponsive and relatively expensive set of launchers in the Expandable, Expendable Launch Vehicles (EELV) platforms; Delta IV and Atlas V. The United States directed Air Force Space Command to develop the capability for operationally responsive access to space and use of space to support national security, including the ability to provide critical space capabilities in the event of a failure of launch or on-orbit capabilities. On 1 Aug 06, Air Force Space Command activated the Space Development & Test Wing (SDTW) to perform development, test and evaluation of Air Force space systems and to execute advanced space deployment and demonstration projects to exploit new concepts and technologies, and rapidly migrate capabilities to the warfighter. The SDTW charged the Launch Test Squadron (LTS) with the mission to develop the capability of small space launch, supporting government research and development space launches and missile defense target missions, with operationally responsive spacelift for Low-Earth-Orbit Space Situational Awareness assets as a future mission. This new mission created new challenges for LTS. The LTS mission tenets of developing space launches and missile defense target vehicles were an evolution from the squadrons previous mission of providing sounding rockets under the Rocket

  15. Magnetic Launch Assist Demonstration Test

    Science.gov (United States)

    2001-01-01

    This image shows a 1/9 subscale model vehicle clearing the Magnetic Launch Assist System, formerly referred to as the Magnetic Levitation (MagLev), test track during a demonstration test conducted at the Marshall Space Flight Center (MSFC). Engineers at MSFC have developed and tested Magnetic Launch Assist technologies. To launch spacecraft into orbit, a Magnetic Launch Assist System would use magnetic fields to levitate and accelerate a vehicle along a track at very high speeds. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, a launch-assist system would electromagnetically drive a space vehicle along the track. A full-scale, operational track would be about 1.5-miles long and capable of accelerating a vehicle to 600 mph in 9.5 seconds. This track is an advanced linear induction motor. Induction motors are common in fans, power drills, and sewing machines. Instead of spinning in a circular motion to turn a shaft or gears, a linear induction motor produces thrust in a straight line. Mounted on concrete pedestals, the track is 100-feet long, about 2-feet wide and about 1.5-feet high. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  16. HIV Conference to Be Held on October 21 at NCI at Frederick | Poster

    Science.gov (United States)

    By Anne Arthur, Guest Writer The HIV Drug Resistance Program Conference on “Virus Structure: Putting the Pieces Together” will be held at NCI at Frederick on October 21, 2014, from 1:00 to 5:45 p.m. in the Conference Center auditorium, Building 549.

  17. 78 FR 19496 - Submission for OMB Review; 30-day Comment Request; The National Cancer Institute (NCI...

    Science.gov (United States)

    2013-04-01

    ... HUMAN SERVICES National Institutes of Health Submission for OMB Review; 30-day Comment Request; The National Cancer Institute (NCI) SmokefreeTXT Program Evaluation SUMMARY: Under the provisions of Section... submitted ] to the Office of Management and Budget (OMB) a request to review and approve the...

  18. 78 FR 44136 - Submission for OMB review; 30-day Comment Request: National Cancer Institute (NCI) Cancer...

    Science.gov (United States)

    2013-07-23

    ... HUMAN SERVICES National Institutes of Health Submission for OMB review; 30-day Comment Request: National Cancer Institute (NCI) Cancer Nanotechnology Platform Partnership Scientific Progress Reports SUMMARY... Institutes of Health (NIH), has submitted to the Office of Management and Budget (OMB) a request for...

  19. Down-Regulation of Bcl-2 Protein Sensitizes NCI 460 Cells to Radiotherapy-Induced Apoptosis

    Institute of Scientific and Technical Information of China (English)

    Dongmei He; Yuan Zhang; Gexiu Liu

    2006-01-01

    OBJECTIVE To determine whether Bcl-2 protein down-regulation can render NCI-460 cells more susceptible to gamma radiation-induced apoptosis by treatment with antisense oligonucleotide (ASODN) against the coding region of Bcl-2 mRNA.METHODS Cell survival was determined using the trypan blue dye exclusion. Expression of the Bcl-2 protein was assayed using immunofluorescence labeling with fluoresce isothiocyanate. Apoptosis was determined by Giemsa staining and flow cytomertry.RESULTS It was found that Bcl-2 ASODN combined with radiation significantly reduced the number of viable cells (P<0.05). There was no difference in cell survival between a nonsense oligodeoxynucleotide/radiation combination and cells treated with radiation alone. Bcl-2 ASODN combined with radiation significantly inhibited expression of the Bcl-2protein in the NCI-H460 cells (P<0.05). Using Giemsa staining, cells treated with Bcl-2 ASODN combined with radiation at 72 h displayed classic apoptotic changes. Apoptotic rates of the NCI-H460 cells treated with Bcl-2 ASODN combined with radiation significantly increased (P<0.05), compared with either a nonsense oligodeoxynucleotide/radiation combination or radiation-treatment cells alone.CONCLUSION ASODN against the coding region of Bcl-2 mRNA increases radiation-induced apoptosis in NCI-H460 cells.

  20. 78 FR 2678 - Proposed Collection; Comment Request (60-Day FRN): The National Cancer Institute (NCI...

    Science.gov (United States)

    2013-01-14

    ... (OMB) for review and approval. Written comments and/or suggestions from the public and affected... comments in writing, request more information on the proposed project, or to obtain a copy of the data... developed (and is managed) by the ] National Cancer Institute (NCI) Tobacco Control Research Branch...

  1. Treatment of Prostate Cancer using Anti-androgen Small Molecules | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    The National Cancer Institute seeks parties interested in collaborative research to co-develop and commercialize a new class of small molecules for the treatment of prostate cancer. General information on co-development research collaborations, can be found on our web site (http://ttc.nci.nih.gov/forms).

  2. NCI and the Chinese National Cancer Center pursue new collaborations in cancer research

    Science.gov (United States)

    CGH Director, Dr. Ted Trimble, and East Asia Program Director, Dr. Ann Chao, traveled to Beijing with Mr. Matthew Brown from the Department of Health and Human Services Office of Global Affairs to attend the Joint Meeting of the NCC and the U.S. NCI.

  3. NCI at Frederick Employees Receive Awards at the Spring Research Festival | Poster

    Science.gov (United States)

    NCI and Frederick National Laboratory staff members were among those honored at the Spring Research Festival Awards Ceremony on May 28. The ceremony was the culmination of the festival, which was sponsored by the National Interagency Confederation for Biological Research (NICBR), May 4–7. Maj. Gen. Brian Lein, commanding general, U.S. Army Medical Research and Materiel Command (USAMRMC), presented the awards.

  4. Reducing Friction: An Update on the NCIP Open Development Initiative - NCI BioMedical Informatics Blog

    Science.gov (United States)

    NCIP has migrated 132 repositories from the NCI subversion repository to our public NCIP GitHub channel with the goal of facilitating third party contributions to the existing code base. Within the GitHub environment, we are advocating use of the GitHub “fork and pull” model.

  5. HIV Conference to Be Held on October 21 at NCI at Frederick | Poster

    Science.gov (United States)

    By Anne Arthur, Guest Writer The HIV Drug Resistance Program Conference on “Virus Structure: Putting the Pieces Together” will be held at NCI at Frederick on October 21, 2014, from 1:00 to 5:45 p.m. in the Conference Center auditorium, Building 549.

  6. Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226

    Institute of Scientific and Technical Information of China (English)

    HAO ZHANG; NA LI; YUE CHEN; LING-YUN HUANG; YI-CHING WANG; GANG FANG; DA-CHENG HE; XUE-YUAN XIAO

    2007-01-01

    Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction,and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis.Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.

  7. The Falcon I Launch Vehicle

    OpenAIRE

    Koenigsmann, Hans; Musk, Elon; Shotwell, Gwynne; Chinnery, Anne

    2004-01-01

    Falcon I is the first in a family of launch vehicles designed by Space Exploration Technologies to facilitate low cost access to space. Falcon I is a mostly reusable, two stage, liquid oxygen and kerosene powered launch vehicle. The vehicle is designed above all for high reliability, followed by low cost and a benign flight environment. Launched from Vandenberg, a standard Falcon I can carry over 1000 lbs to sun-synchronous orbit and 1500 lbs due east to 100 NM. To minimize failure modes, the...

  8. Hewitt launches Research Councils UK

    CERN Multimedia

    2002-01-01

    "Trade and Industry Secretary Patricia Hewitt today launched 'Research Councils UK' - a new strategic partnership that will champion research in science, engineering and technology across the UK" (1 page).

  9. Persistant Launch Range Surveillance Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Launch site infrastructure and space vehicle assets represent multi-billion dollar investments that must be protected. Additionally, personnel and equipment must be...

  10. Magnetic Launch Assist Experimental Track

    Science.gov (United States)

    1999-01-01

    In this photograph, a futuristic spacecraft model sits atop a carrier on the Magnetic Launch Assist System, formerly known as the Magnetic Levitation (MagLev) System, experimental track at the Marshall Space Flight Center (MSFC). Engineers at MSFC have developed and tested Magnetic Launch Assist technologies that would use magnetic fields to levitate and accelerate a vehicle along a track at very high speeds. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, a Magnetic Launch Assist system would electromagnetically drive a space vehicle along the track. A full-scale, operational track would be about 1.5-miles long and capable of accelerating a vehicle to 600 mph in 9.5 seconds. This track is an advanced linear induction motor. Induction motors are common in fans, power drills, and sewing machines. Instead of spinning in a circular motion to turn a shaft or gears, a linear induction motor produces thrust in a straight line. Mounted on concrete pedestals, the track is 100-feet long, about 2-feet wide, and about 1.5-feet high. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  11. STS-53 Launch and Landing

    Science.gov (United States)

    1992-01-01

    Footage of various stages of the STS-53 Discovery launch is shown, including shots of the crew at breakfast, getting suited up, and departing to board the Orbiter. The launch is seen from many vantage points, as is the landing. On-orbit activities show the crew performing several medical experiments, such as taking a picture of the retina and measuring the pressure on the eyeball. One crewmember demonstrates how to use the rowing machine in an antigravity environment.

  12. CubeSat Launch Initiative

    Science.gov (United States)

    Higginbotham, Scott

    2016-01-01

    The National Aeronautics and Space Administration (NASA) recognizes the tremendous potential that CubeSats (very small satellites) have to inexpensively demonstrate advanced technologies, collect scientific data, and enhance student engagement in Science, Technology, Engineering, and Mathematics (STEM). The CubeSat Launch Initiative (CSLI) was created to provide launch opportunities for CubeSats developed by academic institutions, non-profit entities, and NASA centers. This presentation will provide an overview of the CSLI, its benefits, and its results.

  13. CellMiner: a relational database and query tool for the NCI-60 cancer cell lines

    Directory of Open Access Journals (Sweden)

    Reinhold William C

    2009-06-01

    Full Text Available Abstract Background Advances in the high-throughput omic technologies have made it possible to profile cells in a large number of ways at the DNA, RNA, protein, chromosomal, functional, and pharmacological levels. A persistent problem is that some classes of molecular data are labeled with gene identifiers, others with transcript or protein identifiers, and still others with chromosomal locations. What has lagged behind is the ability to integrate the resulting data to uncover complex relationships and patterns. Those issues are reflected in full form by molecular profile data on the panel of 60 diverse human cancer cell lines (the NCI-60 used since 1990 by the U.S. National Cancer Institute to screen compounds for anticancer activity. To our knowledge, CellMiner is the first online database resource for integration of the diverse molecular types of NCI-60 and related meta data. Description CellMiner enables scientists to perform advanced querying of molecular information on NCI-60 (and additional types through a single web interface. CellMiner is a freely available tool that organizes and stores raw and normalized data that represent multiple types of molecular characterizations at the DNA, RNA, protein, and pharmacological levels. Annotations for each project, along with associated metadata on the samples and datasets, are stored in a MySQL database and linked to the molecular profile data. Data can be queried and downloaded along with comprehensive information on experimental and analytic methods for each data set. A Data Intersection tool allows selection of a list of genes (proteins in common between two or more data sets and outputs the data for those genes (proteins in the respective sets. In addition to its role as an integrative resource for the NCI-60, the CellMiner package also serves as a shell for incorporation of molecular profile data on other cell or tissue sample types. Conclusion CellMiner is a relational database tool for

  14. NASA's Space Launch System: Momentum Builds Towards First Launch

    Science.gov (United States)

    May, Todd; Lyles, Garry

    2014-01-01

    NASA's Space Launch System (SLS) is gaining momentum programmatically and technically toward the first launch of a new exploration-class heavy lift launch vehicle for international exploration and science initiatives. The SLS comprises an architecture that begins with a vehicle capable of launching 70 metric tons (t) into low Earth orbit. Its first mission will be the launch of the Orion Multi-Purpose Crew Vehicle (MPCV) on its first autonomous flight beyond the Moon and back. SLS will also launch the first Orion crewed flight in 2021. SLS can evolve to a 130-t lift capability and serve as a baseline for numerous robotic and human missions ranging from a Mars sample return to delivering the first astronauts to explore another planet. Managed by NASA's Marshall Space Flight Center, the SLS Program formally transitioned from the formulation phase to implementation with the successful completion of the rigorous Key Decision Point C review in 2014. At KDP-C, the Agency Planning Management Council determines the readiness of a program to go to the next life-cycle phase and makes technical, cost, and schedule commitments to its external stakeholders. As a result, the Agency authorized the Program to move forward to Critical Design Review, scheduled for 2015, and a launch readiness date of November 2018. Every SLS element is currently in testing or test preparations. The Program shipped its first flight hardware in 2014 in preparation for Orion's Exploration Flight Test-1 (EFT-1) launch on a Delta IV Heavy rocket in December, a significant first step toward human journeys into deep space. Accomplishments during 2014 included manufacture of Core Stage test articles and preparations for qualification testing the Solid Rocket Boosters and the RS-25 Core Stage engines. SLS was conceived with the goals of safety, affordability, and sustainability, while also providing unprecedented capability for human exploration and scientific discovery beyond Earth orbit. In an environment

  15. Chinasat 9 to Be Launched in 2007

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    CASC is chosen by China Satellite Communications Corporation (China Satcom) to launch Chinasat 9 direct broadcasting satellite around July 2007, The satellite will be launched into a geostationary orbit by a LM3B from Xichang Satellite Launch Center,

  16. Global microRNA analysis of the NCI-60 cancer cell panel

    DEFF Research Database (Denmark)

    Søkilde, Rolf; Kaczkowski, Bogumil; Podolska, Agnieszka

    2011-01-01

    MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation...... and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 955 miRNAs in the NCI-60 cancer cell lines and identified tissue- and cell......-type-specific miRNA patterns by unsupervised hierarchical clustering and statistical analysis. A comparison of our data to three previously published miRNA expression studies on the NCI-60 panel showed a remarkably high correlation between the different technical platforms. In addition, the current work...

  17. Methodology of Establishing and Identifying NCI-H2228/Crizotinib-resistant Cell Lines In Vitro

    Directory of Open Access Journals (Sweden)

    Di WU

    2015-06-01

    Full Text Available Background and objective The mechanisms of small molecule targeting drug resistance and ways to overcome resistance are now both urgent need to improve the clinical efficacy. This study aimed to investigate the feasibility of using different methods to establish the crizotinib-resistant non-small cell lung cancer NCI-H2228/Crizotinib cell lines and to clarify the mechanisms of resistance to small molecule targeting drug, thus providing experimental and theoretical bases for further studies to overcome the mechanisms of Crizotinib resistance. Methods The study utilized stepwise increase of drug concentrations and chemical mutagen to induce Crizotinib-resistant NCI-H2228 cells. The drug 50% inhibitory concentration (IC50 values of parental and resistant cells and the population doubling time were determined by MTT assay. The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK expression was evaluated by RT-PCR and Western blot. Full-length sequencing method was used to compare the EML4-ALK genes in the parent and drug-resistant cells and analyze the mechanisms of drug resistance. Results The method of gradually increasing drug concentration to induce Crizotinib-resistant NCI-H2228 cells was time-consuming because the cell growth recovery was extremely slow. Thus, this method was considered invalid. However, chemical mutagen ENU can effectively induce NCI-H2228 cells resistant to crizotinib in a short time [IC50]= (3.810±1.100 μmol/L, P=0.002,9 vs parental cells]. Furthermore, the gene mutation frequency of EML4-ALK in the resistant cells was significantly higher than that in the parent cells. Conclusion Chemical mutagen-induced cell resistance was easily operated and had effectively shortened the experimental process. Preliminary technical methods and experimental evidence for in-depth study of drug resistance mechanisms and approaches to overcome the targeted drug resistance were also provided.

  18. CellMiner Companion: an interactive web application to explore CellMiner NCI-60 data.

    Science.gov (United States)

    Wang, Sufang; Gribskov, Michael; Hazbun, Tony R; Pascuzzi, Pete E

    2016-08-01

    The NCI-60 human tumor cell line panel is an invaluable resource for cancer researchers, providing drug sensitivity, molecular and phenotypic data for a range of cancer types. CellMiner is a web resource that provides tools for the acquisition and analysis of quality-controlled NCI-60 data. CellMiner supports queries of up to 150 drugs or genes, but the output is an Excel file for each drug or gene. This output format makes it difficult for researchers to explore the data from large queries. CellMiner Companion is a web application that facilitates the exploration and visualization of output from CellMiner, further increasing the accessibility of NCI-60 data. The web application is freely accessible at https://pul-bioinformatics.shinyapps.io/CellMinerCompanion The R source code can be downloaded at https://github.com/pepascuzzi/CellMinerCompanion.git ppascuzz@purdue.edu Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  19. Computational Challenges and Collaborative Projects in the NCI Quantitative Imaging Network.

    Science.gov (United States)

    Farahani, Keyvan; Kalpathy-Cramer, Jayashree; Chenevert, Thomas L; Rubin, Daniel L; Sunderland, John J; Nordstrom, Robert J; Buatti, John; Hylton, Nola

    2016-12-01

    The Quantitative Imaging Network (QIN) of the National Cancer Institute (NCI) conducts research in development and validation of imaging tools and methods for predicting and evaluating clinical response to cancer therapy. Members of the network are involved in examining various imaging and image assessment parameters through network-wide cooperative projects. To more effectively use the cooperative power of the network in conducting computational challenges in benchmarking of tools and methods and collaborative projects in analytical assessment of imaging technologies, the QIN Challenge Task Force has developed policies and procedures to enhance the value of these activities by developing guidelines and leveraging NCI resources to help their administration and manage dissemination of results. Challenges and Collaborative Projects (CCPs) are further divided into technical and clinical CCPs. As the first NCI network to engage in CCPs, we anticipate a variety of CCPs to be conducted by QIN teams in the coming years. These will be aimed to benchmark advanced software tools for clinical decision support, explore new imaging biomarkers for therapeutic assessment, and establish consensus on a range of methods and protocols in support of the use of quantitative imaging to predict and assess response to cancer therapy.

  20. Canadian Space Launch: Exploiting Northern Latitudes For Efficient Space Launch

    Science.gov (United States)

    2015-04-01

    facility and therefore has some basic infrastructure in place. It housed 4,500 personnel and launched over 3,500 sub-orbital flights.49 While the... Melbourne , FL. http://www.spaceops2012.org/ proceedings/documents/id1295313- Paper-002.pdf (accessed 10 Mar 2015). Winters, Nathan J. “Enabling the

  1. LM-2C Series Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    XueFuxing

    2004-01-01

    On December 30, 2003, a LM-2C/SM launch vehicle was launched from Xichang Satellite Launch Center (XSLC), successfully sending TC-1 satellite into orbit. The satellite is the first one of the two scientific satellites known as Double Star. The operation orbit of the satellite is the highest compared with China's other satellites ever launched.

  2. Healthy Border 2020 Embassy Launch

    Science.gov (United States)

    The U.S.-Mexico Border Health Commission launched the Healthy Border 2020 at the Mexican Embassy in the United States on June 24, 2015. This new initiative aims to strengthening what was accomplished on the previous plan of action entitled Healthy Border 2010.

  3. Evolved Expendable Launch Vehicle (EELV)

    Science.gov (United States)

    2015-12-15

    2015. When the draft RFP was posted a Space and Missile Systems Center (SMC) internal Comment Resolution Matrix ( CRM ) was inadvertently exposed for...competitiveness; and benefit the U.S. economy. The overall goal of the EELV December 2015 SAR March 14, 2016 16:05:14 UNCLASSIFIED 8 launch system investment is

  4. Prospects For China's Expendable Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    Long Lehao; Wang Xiaojun; Rong Yi

    2009-01-01

    @@ The expendable launch vehicle ( ELV) is the major means for human beings to enter space. Up until April 2009, China's Long March (LM) series launch vehicle has conducted 117 launches, and realized 75 consecutive successful launches since October 1996, which marks China's ELV development has entered a new historical era. Based on the analysis of China's LM series launch vehicle development status, combining with the new generation launch vehicle development, this raises a development prospect for China's ELV to meet the demands for future launch vehicle technology development.

  5. NASA's Space Launch System: Momentum Builds Toward First Launch

    Science.gov (United States)

    May, Todd A.; Lyles, Garry M.

    2014-01-01

    NASA's Space Launch System (SLS) is gaining momentum toward the first launch of a new exploration-class heavy lift launch vehicle for international exploration and science initiatives. The SLS comprises an architecture that begins with a vehicle capable of launching 70 metric tons (t) into low Earth orbit. It will launch the Orion Multi-Purpose Crew Vehicle (MPCV) on its first autonomous flight beyond the Moon and back in December 2017. Its first crewed flight follows in 2021. SLS can evolve to a130-t lift capability and serve as a baseline for numerous robotic and human missions ranging from a Mars sample return to delivering the first astronauts to explore another planet. The SLS Program formally transitioned from the formulation phase to implementation with the successful completion of the rigorous Key Decision Point C review in 2014. As a result, the Agency authorized the Program to move forward to Critical Design Review, scheduled for 2015. In the NASA project life cycle process, SLS has completed 50 percent of its major milestones toward first flight. Every SLS element manufactured development hardware for testing over the past year. Accomplishments during 2013/2014 included manufacture of core stage test articles, preparations for qualification testing the solid rocket boosters and the RS-25 main engines, and shipment of the first flight hardware in preparation for the Exploration Flight Test-1 (EFT-1) in 2014. SLS was conceived with the goals of safety, affordability, and sustainability, while also providing unprecedented capability for human exploration and scientific discovery beyond Earth orbit. In an environment of economic challenges, the SLS team continues to meet ambitious budget and schedule targets through the studied use of hardware, infrastructure, and workforce investments the United States made in the last half century, while selectively using new technologies for design, manufacturing, and testing, as well as streamlined management approaches

  6. NASA's Space Launch System: Moving Toward the Launch Pad

    Science.gov (United States)

    Creech, Stephen D.; May, Todd A.

    2013-01-01

    The National Aeronautics and Space Administration's (NASA's) Space Launch System (SLS) Program, managed at the Marshall Space Flight Center (MSFC), is making progress toward delivering a new capability for human space flight and scientific missions beyond Earth orbit. Designed with the goals of safety, affordability, and sustainability in mind, the SLS rocket will launch the Orion Multi-Purpose Crew Vehicle (MPCV), equipment, supplies, and major science missions for exploration and discovery. Supporting Orion's first autonomous flight to lunar orbit and back in 2017 and its first crewed flight in 2021, the SLS will evolve into the most powerful launch vehicle ever flown via an upgrade approach that will provide building blocks for future space exploration. NASA is working to deliver this new capability in an austere economic climate, a fact that has inspired the SLS team to find innovative solutions to the challenges of designing, developing, fielding, and operating the largest rocket in history. This paper will summarize the planned capabilities of the vehicle, the progress the SLS Program has made in the 2 years since the Agency formally announced its architecture in September 2011, the path it is following to reach the launch pad in 2017 and then to evolve the 70 metric ton (t) initial lift capability to 130-t lift capability after 2021. The paper will explain how, to meet the challenge of a flat funding curve, an architecture was chosen that combines the use and enhancement of legacy systems and technology with strategic new developments that will evolve the launch vehicle's capabilities. This approach reduces the time and cost of delivering the initial 70 t Block 1 vehicle, and reduces the number of parallel development investments required to deliver the evolved 130 t Block 2 vehicle. The paper will outline the milestones the program has already reached, from developmental milestones such as the manufacture of the first flight hardware, to life

  7. National Security Space Launch Report

    Science.gov (United States)

    2006-01-01

    widely used, with applications in wireless ground communications, authentication of electronic transactions, and management of large computerized networks ...flight of the Ariane 5G in 1996),23 cost is a major factor in the program’s success. The launch costs for an Ariane 5 are in the $130 million to $150...Knauf, Deputy Director, Directorate of Space Acquisition (SAF/USA). VADM James D. McArthur, Jr., Commander, Naval Network Warfare Command—“Naval Space

  8. Former WHK Intern Returns to NCI at Frederick as Earl-Stadtman Investigator | Poster

    Science.gov (United States)

    The Laboratory of Cell and Developmental Signaling (LCDS) recently welcomed John Brognard, Ph.D., as the new Earl-Stadtman Investigator. While Brognard is new to this role, he is not new to NCI at Frederick. In high school, Brognard was a Werner H. Kirsten Student Intern in what was formerly known as the ABL research program, where he worked under Bob Moschel, Ph.D., senior investigator, and Gary Pauly, Ph.D., currently a staff scientist in the Chemical Biology Laboratory.

  9. Test de visualitat: les preferències del bon disseny

    Directory of Open Access Journals (Sweden)

    Quim Merino

    2014-07-01

    Full Text Available Aquest article pretén donar notícia de la investigació dirigida pel Grup de Recerca en Publicitat i Relacions Públiques (en endavant, GRP de la Universitat Autònoma de Barcelona duta a terme pels autors d'aquesta ressenya. El treball s'emmarca en una activitat de l'assignatura de Disseny en Publicitat i Relacions Públiques del Grau en Publicitat i Relacions Públiques de la UAB. L'objectiu del treball és constatar les preferències del consumidor davant diferents estímuls formals del disseny gràfic en publicitat

  10. Microsoft Office 365 Deployment Continues through June at NCI at Frederick | Poster

    Science.gov (United States)

    The latest Microsoft suite, Office 365 (O365), is being deployed to all NCI at Frederick computers during the months of May and June to comply with federal mandates. The suite includes the latest versions of Word, Excel, Outlook, PowerPoint, and Skype for Business, along with cloud-based capabilities. These cloud-based capabilities will help meet the federal mandates that require all Health and Human Services operating divisions to migrate e-mail to the cloud by the end of 2016.

  11. Launch Services, a Proven Model

    Science.gov (United States)

    Trafton, W. C.; Simpson, J.

    2002-01-01

    From a commercial perspective, the ability to justify "leap frog" technology such as reusable systems has been difficult to justify because the estimated 5B to 10B investment is not supported in the current flat commercial market coupled with an oversupply of launch service suppliers. The market simply does not justify investment of that magnitude. Currently, next generation Expendable Launch Systems, including Boeing's Delta IV, Lockheed Martin's Atlas 5, Ariane V ESCA and RSC's H-IIA are being introduced into operations signifying that only upgrades to proven systems are planned to meet the changes in anticipated satellite demand (larger satellites, more lifetime, larger volumes, etc.) in the foreseeable future. We do not see a new fleet of ELVs emerging beyond that which is currently being introduced, only continuous upgrades of the fleet to meet the demands. To induce a radical change in the provision of launch services, a Multinational Government investment must be made and justified by World requirements. The commercial market alone cannot justify such an investment. And if an investment is made, we cannot afford to repeat previous mistakes by relying on one system such as shuttle for commercial deployment without having any back-up capability. Other issues that need to be considered are national science and security requirements, which to a large extent fuels the Japanese, Chinese, Indian, Former Soviet Union, European and United States space transportation entries. Additionally, this system must support or replace current Space Transportation Economies with across-the-board benefits. For the next 10 to 20 years, Multinational cooperation will be in the form of piecing together launch components and infrastructure to supplement existing launch systems and reducing the amount of non-recurring investment while meeting the future requirements of the End-User. Virtually all of the current systems have some form of multinational participation: Sea Launch

  12. Enabling Technology for Small Satellite Launch Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Access to space for Small Satellites is enabled by the use of excess launch capacity on existing launch vehicles. A range of sizes, form factors and masses of small...

  13. A method for analyzing strategic product launch

    OpenAIRE

    XIAO Junji

    2007-01-01

    This paper proposes a method to analyze how the manufacturers make product launch decisions in a multi-product oligopoly market, and how the heterogeneity in their products affects the manufacturers' decisions on model launch and withdrawal.

  14. Enabling Technology for Small Satellite Launch Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Access to space for Small Satellites is enabled by the use of excess launch capacity on existing launch vehicles. A range of sizes, form factors and masses need to...

  15. NCI Workshop Report: Clinical and Computational Requirements for Correlating Imaging Phenotypes with Genomics Signatures

    Directory of Open Access Journals (Sweden)

    Rivka Colen

    2014-10-01

    Full Text Available The National Cancer Institute (NCI Cancer Imaging Program organized two related workshops on June 26–27, 2013, entitled “Correlating Imaging Phenotypes with Genomics Signatures Research” and “Scalable Computational Resources as Required for Imaging-Genomics Decision Support Systems.” The first workshop focused on clinical and scientific requirements, exploring our knowledge of phenotypic characteristics of cancer biological properties to determine whether the field is sufficiently advanced to correlate with imaging phenotypes that underpin genomics and clinical outcomes, and exploring new scientific methods to extract phenotypic features from medical images and relate them to genomics analyses. The second workshop focused on computational methods that explore informatics and computational requirements to extract phenotypic features from medical images and relate them to genomics analyses and improve the accessibility and speed of dissemination of existing NIH resources. These workshops linked clinical and scientific requirements of currently known phenotypic and genotypic cancer biology characteristics with imaging phenotypes that underpin genomics and clinical outcomes. The group generated a set of recommendations to NCI leadership and the research community that encourage and support development of the emerging radiogenomics research field to address short-and longer-term goals in cancer research.

  16. Receptor-Based Virtual Screening of EGFR Kinase Inhibitors from the NCI Diversity Database

    Directory of Open Access Journals (Sweden)

    Kiattawee Choowongkomon

    2010-06-01

    Full Text Available Epidermal growth factor receptor (EGFR abnormalities have been associated with several types of human cancer. The crystal structures of its tyrosine kinase domain (EGFR-TK complexed with small molecule inhibitors revealed the kinase inhibition modes, prompting us to search for novel anti-cancer drugs. A total of 1,990 compounds from the National Cancer Institute (NCI diversity set with nonredundant structures have been tested to inhibit cancer cell lines with unknown mechanism. Cancer inhibition through EGFR-TK is one of the mechanisms of these compounds. In this work, we performed receptor-based virtual screening against the NCI diversity database. Using two different docking algorithms, AutoDock and Gold, combined with subsequent post-docking analyses, we found eight candidate compounds with high scoring functions that all bind to the ATP-competitive site of the kinase. None of these compounds belongs to the main group of the currently known EGFR-TK inhibitors. Binding mode analyses revealed that the way these compounds complexed with EGFR-TK differs from quinazoline inhibitor binding and the interaction mainly involves hydrophobic interactions. Also, the common kinase-inhibitor (NH---N and CO---HC hydrogen bonds between the hinge region and the hit compounds are rarely observed. Our results suggest that these molecules could be developed as novel lead compounds in anti-cancer drug design.

  17. New product development and product launch strategies

    OpenAIRE

    Filiz Bozkurt Bekoğlu; Ahu Ergen

    2016-01-01

    In today’s highly competitive environment, a balanced product portfolio, success in new product development and product launch are important factors for the sustainability of organizations. The aim of the study is to reveal the right product launch steps for the companies through theory and case study. In the study, new product development and product launch strategies are first investigated theoretically. Afterwards, a successful product series launch case from cosmetics sector is analyzed. ...

  18. Role of AMP-activated protein kinase on steroid hormone biosynthesis in adrenal NCI-H295R cells.

    Directory of Open Access Journals (Sweden)

    Andrea Hirsch

    Full Text Available Regulation of human androgen biosynthesis is poorly understood. However, detailed knowledge is needed to eventually solve disorders with androgen dysbalance. We showed that starvation growth conditions shift steroidogenesis of human adrenal NCI-H295R cells towards androgen production attributable to decreased HSD3B2 expression and activity and increased CYP17A1 phosphorylation and 17,20-lyase activity. Generally, starvation induces stress and energy deprivation that need to be counteracted to maintain proper cell functions. AMP-activated protein kinase (AMPK is a master energy sensor that regulates cellular energy balance. AMPK regulates steroidogenesis in the gonad. Therefore, we investigated whether AMPK is also a regulator of adrenal steroidogenesis. We hypothesized that starvation uses AMPK signaling to enhance androgen production in NCI-H295R cells. We found that AMPK subunits are expressed in NCI-H295 cells, normal adrenal tissue and human as well as pig ovary cells. Starvation growth conditions decreased phosphorylation, but not activity of AMPK in NCI-H295 cells. In contrast, the AMPK activator 5-aminoimidazole-4-carboxamide (AICAR increased AMPKα phosphorylation and increased CYP17A1-17,20 lyase activity. Compound C (an AMPK inhibitor, directly inhibited CYP17A1 activities and can therefore not be used for AMPK signaling studies in steroidogenesis. HSD3B2 activity was neither altered by AICAR nor compound C. Starvation did not affect mitochondrial respiratory chain function in NCI-H295R cells suggesting that there is no indirect energy effect on AMPK through this avenue. In summary, starvation-mediated increase of androgen production in NCI-H295 cells does not seem to be mediated by AMPK signaling. But AMPK activation can enhance androgen production through a specific increase in CYP17A1-17,20 lyase activity.

  19. THE HARBOUR DEFENCE MOTOR LAUNCHES

    Directory of Open Access Journals (Sweden)

    W.H. Rice

    2012-02-01

    Full Text Available One of the handiest small craft to emerge from the Second World War was the 72 fet Harbour Defence Motor Launch. It's purpose was to patrol harbours and their approaches and to guard against attack by swimmers or underwater vehicles such as 'chariots' or even submarines. For this task the craft was fitted with a small ASDIC outfit and carried eight depth charges. Surface armament comprised a three-pounder gun on the foredeck, twin Lewis guns on the bridge and a 20 mm Oerlikon aft.

  20. Launch vehicle systems design analysis

    Science.gov (United States)

    Ryan, Robert; Verderaime, V.

    1993-01-01

    Current launch vehicle design emphasis is on low life-cycle cost. This paper applies total quality management (TQM) principles to a conventional systems design analysis process to provide low-cost, high-reliability designs. Suggested TQM techniques include Steward's systems information flow matrix method, quality leverage principle, quality through robustness and function deployment, Pareto's principle, Pugh's selection and enhancement criteria, and other design process procedures. TQM quality performance at least-cost can be realized through competent concurrent engineering teams and brilliance of their technical leadership.

  1. GRYPHON: Air launched space booster

    Science.gov (United States)

    1993-06-01

    The project chosen for the winter semester Aero 483 class was the design of a next generation Air Launched Space Booster. Based on Orbital Sciences Corporation's Pegasus concept, the goal of Aero 483 was to design a 500,000 pound air launched space booster capable of delivering 17,000 pounds of payload to Low Earth Orbit and 8,000 pounds of payload to Geosynchronous Earth Orbit. The resulting launch vehicle was named the Gryphon. The class of forty senior aerospace engineering students was broken down into eight interdependent groups. Each group was assigned a subsystem or responsibility which then became their field of specialization. Spacecraft Integration was responsible for ensuring compatibility between subsystems. This group kept up to date on subsystem redesigns and informed those parties affected by the changes, monitored the vehicle's overall weight and dimensions, and calculated the mass properties of the booster. This group also performed the cost/profitability analysis of the Gryphon and obtained cost data for competing launch systems. The Mission Analysis Group was assigned the task of determining proper orbits, calculating the vehicle's flight trajectory for those orbits, and determining the aerodynamic characteristics of the vehicle. The Propulsion Group chose the engines that were best suited to the mission. This group also set the staging configurations for those engines and designed the tanks and fuel feed system. The commercial satellite market, dimensions and weights of typical satellites, and method of deploying satellites was determined by the Payloads Group. In addition, Payloads identified possible resupply packages for Space Station Freedom and identified those packages that were compatible with the Gryphon. The guidance, navigation, and control subsystems were designed by the Mission Control Group. This group identified required tracking hardware, communications hardware telemetry systems, and ground sites for the location of the Gryphon

  2. Closed End Launch Tube (CELT)

    Science.gov (United States)

    Lueck, Dale E.; Immer, Christopher D.

    2004-02-01

    A small-scale test apparatus has been built and tested for the CELT pneumatic launch assist concept presented at STAIF 2001. The 7.5 cm (3-inch) diameter × 305 M (1000 feet) long system accelerates and pneumatically brakes a 6.35 cm diameter projectile with variable weight (1.5 - 5 Kg). The acceleration and braking tube has been instrumented with optical sensors and pressure transducers at 14 stations to take data throughout the runs. Velocity and pressure profiles for runs with various accelerator pressures and projectile weights are given. This test apparatus can serve as an important experimental tool for verifying this concept.

  3. Drift wave launching in a linear quadrupole

    Energy Technology Data Exchange (ETDEWEB)

    Tessema, G.Y.; Elliott, J.A.; Rusbridge, M.G. (Manchester Univ. (UK). Inst. of Science and Technology)

    1989-12-01

    Drift waves have been successfully launched from flag probes in a steady-state magnetized plasma, and the launching mechanism has been identified. Non-linear interactions are observed between launched and intrinsic waves. A wide range of further experimental studies is thus made possible, of fundamental relevance to plasma confinement. (author).

  4. How supernovae launch galactic winds?

    Science.gov (United States)

    Fielding, Drummond; Quataert, Eliot; Martizzi, Davide; Faucher-Giguère, Claude-André

    2017-09-01

    We use idealized three-dimensional hydrodynamic simulations of global galactic discs to study the launching of galactic winds by supernovae (SNe). The simulations resolve the cooling radii of the majority of supernova remnants (SNRs) and thus self-consistently capture how SNe drive galactic winds. We find that SNe launch highly supersonic winds with properties that agree reasonably well with expectations from analytic models. The energy loading (η _E= \\dot{E}_wind/ \\dot{E}_SN) of the winds in our simulations are well converged with spatial resolution while the wind mass loading (η _M= \\dot{M}_wind/\\dot{M}_\\star) decreases with resolution at the resolutions we achieve. We present a simple analytic model based on the concept that SNRs with cooling radii greater than the local scaleheight break out of the disc and power the wind. This model successfully explains the dependence (or lack thereof) of ηE (and by extension ηM) on the gas surface density, star formation efficiency, disc radius and the clustering of SNe. The winds our simulations are weaker than expected in reality, likely due to the fact that we seed SNe preferentially at density peaks. Clustering SNe in time and space substantially increases the wind power.

  5. 14 CFR 1214.117 - Launch and orbit parameters for a standard launch.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Launch and orbit parameters for a standard..., Reimbursable Customers § 1214.117 Launch and orbit parameters for a standard launch. To qualify for the...) Launch from Kennedy Space Center (KSC) into the customer's choice of two standard mission orbits: 160...

  6. Magnetic Launch Assist Vehicle-Artist's Concept

    Science.gov (United States)

    1999-01-01

    This artist's concept depicts a Magnetic Launch Assist vehicle clearing the track and shifting to rocket engines for launch into orbit. The system, formerly referred as the Magnetic Levitation (MagLev) system, is a launch system developed and tested by Engineers at the Marshall Space Flight Center (MSFC) that could levitate and accelerate a launch vehicle along a track at high speeds before it leaves the ground. Using an off-board electric energy source and magnetic fields, a Magnetic Launch Assist system would drive a spacecraft along a horizontal track until it reaches desired speeds. The system is similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway. A full-scale, operational track would be about 1.5-miles long, capable of accelerating a vehicle to 600 mph in 9.5 seconds, and the vehicle would then shift to rocket engines for launch into orbit. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  7. Female condom launched in UK.

    Science.gov (United States)

    1992-01-01

    The lone-awaited female condom, Femidom, is to be launched at the end of September by manufacturers Chartex. It is being welcomed by the FPA [Family Planning Association] and other family planning experts as a valuable addition to the existing range of contraceptive methods and as an alternative to the male condom in offering effective protection against sexually transmitted diseases including HIV. A lubricated, loose-fitting polyurethane sheath, Femidom is inserted into the vagina at any time before sex. An inner ring holds the condom in place beyond the pubic bone and an outer ring lies flat against the vulva. In addition to extending choice, it is under direct control of the woman. As FPA Director Doreen Massey puts it: "We have to face the fact that some women who want safer sex can't get their partners to use condoms. for the 1st time with Femidom, you can insist that if he won't use a condom, you'll use yours." In trails of self-selected couples, up to 2/3 of women and their partners found the product acceptable. a study at the Institute of Population Studies in Exeter showed that while some couples had initial misgivings about the condom's size and appearance, especially its visibility when in position, these often declined with repeated use. Researcher Dr. Nicholas Ford pointed out that if the female condom makes a woman feel unattractive, her partner's comments may well influence these feelings. Users' experience of insertion and the condom's comfort also improved with repeated use. While there are no large studies showing ranges of effectiveness, it is likely to be as effective as the male condom (about 85%-98%). In a study of 106 women at the margaret Pyke Center in London, there were 7 unplanned pregnancies: 4 were due to inconsistent use of the method and 3 were method failures. Breakages were rare. 1/3 of participants dropped out in the 1st month. Users should continue with their existing contraceptive method until they are sure that they are using

  8. CERN & Society launches donation portal

    CERN Document Server

    Cian O'Luanaigh

    2014-01-01

    The CERN & Society programme brings together projects in the areas of education and outreach, innovation and knowledge exchange, and culture and arts, that spread the CERN spirit of scientific curiosity for the inspiration and benefit of society. Today, CERN & Society is launching its "giving" website – a portal to allow donors to contribute to various projects and forge new relationships with CERN.   "The CERN & Society initiative in its embryonic form began almost three years ago, with the feeling that the laboratory could play a bigger role for the benefit of society," says Matteo Castoldi, Head of the CERN Development Office, who, with his team, is seeking supporters and ambassadors for the CERN & Society initiative. "The concept is not completely new – in some sense it is embedded in CERN’s DNA, as the laboratory helps society by creating knowledge and new technologies – but we would like to d...

  9. EADS Roadmap for Launch Vehicles

    Science.gov (United States)

    Eymar, Patrick; Grimard, Max

    2002-01-01

    still think about the future, especially at industry level in order to make the most judicious choices in technologies, vehicle types as well as human resources and facilities specialization (especially after recent merger moves). and production as prime contractor, industrial architect or stage provider have taken benefit of this expertise and especially of all the studies ran under national funding and own financing on reusable vehicles and ground/flight demonstrators have analyzed several scenarios. VEHICLES/ASTRIUM SI strategy w.r.t. launch vehicles for the two next decades. Among the main inputs taken into account of course visions of the market evolutions have been considered, but also enlargement of international cooperations and governments requests and supports (e.g. with the influence of large international ventures). 1 patrick.eymar@lanceurs.aeromatra.com 2

  10. Plant collecting program in Southeast Asia under the sponsorship of the United States National Cancer Institute (NCI) (1986-1991)

    NARCIS (Netherlands)

    Soejarto, D.D.

    1992-01-01

    Under the funding from the United States National Cancer Institute (NCI)¹, a program was undertaken to collect plant samples in Southeast Asia to be tested for their cancer- and AIDS-arresting properties, for the period of September 1, 1986 through August 31, 1991. The program was implemented with

  11. New NCI-N87-derived human gastric epithelial line after human telomerase catalytic subunit over-expression

    Science.gov (United States)

    Saraiva-Pava, Kathy; Navabi, Nazanin; Skoog, Emma C; Lindén, Sara K; Oleastro, Mónica; Roxo-Rosa, Mónica

    2015-01-01

    AIM: To establish a cellular model correctly mimicking the gastric epithelium to overcome the limitation in the study of Helicobacter pylori (H. pylori) infection. METHODS: Aiming to overcome this limitation, clones of the heterogenic cancer-derived NCI-N87 cell line were isolated, by stably-transducing it with the human telomerase reverse-transcriptase (hTERT) catalytic subunit gene. The clones were first characterized regarding their cell growth pattern and phenotype. For that we measured the clones’ adherence properties, expression of cell-cell junctions’ markers (ZO-1 and E-cadherin) and ability to generate a sustained transepithelial electrical resistance. The gastric properties of the clones, concerning expression of mucins, zymogens and glycan contents, were then evaluated by haematoxylin and eosin staining, Periodic acid Schiff (PAS) and PAS/Alcian Blue-staining, immunocytochemistry and Western blot. In addition, we assessed the usefulness of the hTERT-expressing gastric cell line for H. pylori research, by performing co-culture assays and measuring the IL-8 secretion, by ELISA, upon infection with two H. pylori strains differing in virulence. RESULTS: Compared with the parental cell line, the most promising NCI-hTERT-derived clones (CL5 and CL6) were composed of cells with homogenous phenotype, presented higher relative telomerase activities, better adhesion properties, ability to be maintained in culture for longer periods after confluency, and were more efficient in PAS-reactive mucins secretion. Both clones were shown to produce high amounts of MUC1, MUC2 and MUC13. NCI-hTERT-CL5 mucins were shown to be decorated with blood group H type 2 (BG-H), Lewis-x (Lex), Ley and Lea and, in a less extent, with BG-A antigens, but the former two antigens were not detected in the NCI-hTERT-CL6. None of the clones exhibited detectable levels of MUC6 nor sialylated Lex and Lea glycans. Entailing good gastric properties, both NCI-hTERT-clones were found to produce

  12. NCI's High Performance Computing (HPC) and High Performance Data (HPD) Computing Platform for Environmental and Earth System Data Science

    Science.gov (United States)

    Evans, Ben; Allen, Chris; Antony, Joseph; Bastrakova, Irina; Gohar, Kashif; Porter, David; Pugh, Tim; Santana, Fabiana; Smillie, Jon; Trenham, Claire; Wang, Jingbo; Wyborn, Lesley

    2015-04-01

    The National Computational Infrastructure (NCI) has established a powerful and flexible in-situ petascale computational environment to enable both high performance computing and Data-intensive Science across a wide spectrum of national environmental and earth science data collections - in particular climate, observational data and geoscientific assets. This paper examines 1) the computational environments that supports the modelling and data processing pipelines, 2) the analysis environments and methods to support data analysis, and 3) the progress so far to harmonise the underlying data collections for future interdisciplinary research across these large volume data collections. NCI has established 10+ PBytes of major national and international data collections from both the government and research sectors based on six themes: 1) weather, climate, and earth system science model simulations, 2) marine and earth observations, 3) geosciences, 4) terrestrial ecosystems, 5) water and hydrology, and 6) astronomy, social and biosciences. Collectively they span the lithosphere, crust, biosphere, hydrosphere, troposphere, and stratosphere. The data is largely sourced from NCI's partners (which include the custodians of many of the major Australian national-scale scientific collections), leading research communities, and collaborating overseas organisations. New infrastructures created at NCI mean the data collections are now accessible within an integrated High Performance Computing and Data (HPC-HPD) environment - a 1.2 PFlop supercomputer (Raijin), a HPC class 3000 core OpenStack cloud system and several highly connected large-scale high-bandwidth Lustre filesystems. The hardware was designed at inception to ensure that it would allow the layered software environment to flexibly accommodate the advancement of future data science. New approaches to software technology and data models have also had to be developed to enable access to these large and exponentially

  13. (Updated) NCI Fiscal 2016 Bypass Budget Proposes $25 Million for Frederick National Lab | Poster

    Science.gov (United States)

    By Nancy Parrish, Staff Writer; image by Richard Frederickson, Staff Photographer The additional funding requested for Frederick National Laboratory for Cancer Research (FNLCR) in the Fiscal 2016 Bypass Budget was $25 million, or approximately 3.5 percent of the total additional funding request of $715 million. Officially called the Professional Judgment Budget, the Bypass Budget is a result of the National Cancer Act of 1971, which authorizes NCI to submit a budget directly to the president, to send to Congress. With a focus on NCI’s research priorities and areas of cancer research with potential for investment, the Bypass Budget specifies additional funding, over and above the current budget, that is needed to advance

  14. AACR-NCI-EORTC--21st International Symposium. Molecular targets and cancer therapeutics--Part 2.

    Science.gov (United States)

    Walker, Karen; Padhiar, Meenal

    2010-01-01

    The 21st international symposium of the American Association for Cancer Research (AACR), the NCI and the European Organization for Research and Treatment of Cancer (EORTC), held in Boston, included topics covering the development of therapeutics and molecular targets in the field of cancer research. This conference report highlights selected presentations on the development of novel drugs for cancer. Investigational drugs discussed include RO-506876 (F Hoffmann-La Roche Ltd), GDC-0980 (Genentech Inc), EMD-1214063 and EMD-1204831 (Merck Serono SA), AR-mTOR01 and AR-mTOR-26 (Array BioPharma Inc), GSK-2126458 (GlaxoSmithKline plc), EXEL-1415 and EXEL-2008 (Exelixis Inc), FP-1039 (FivePrime Therapeutics Inc), and AV-412 (AVEO Pharmaceuticals Inc).

  15. (Updated) NCI Fiscal 2016 Bypass Budget Proposes $25 Million for Frederick National Lab | Poster

    Science.gov (United States)

    By Nancy Parrish, Staff Writer; image by Richard Frederickson, Staff Photographer The additional funding requested for Frederick National Laboratory for Cancer Research (FNLCR) in the Fiscal 2016 Bypass Budget was $25 million, or approximately 3.5 percent of the total additional funding request of $715 million. Officially called the Professional Judgment Budget, the Bypass Budget is a result of the National Cancer Act of 1971, which authorizes NCI to submit a budget directly to the president, to send to Congress. With a focus on NCI’s research priorities and areas of cancer research with potential for investment, the Bypass Budget specifies additional funding, over and above the current budget, that is needed to advance

  16. Effects of emodin on gene expression profile in small cell lung cancer NCI-H446 cells

    Institute of Scientific and Technical Information of China (English)

    FU Zhong-yan; HAN Jin-xiang; HUANG Hai-yan

    2007-01-01

    Background The treatment of patients with small cell lung cancer (SCLC) is based on chemotherapy. However, the treatment is limited by the development of drug resistance. Emodin has been shown to exhibit an anti-cancer effect. But the molecular mechanism remains unclear. This study was conducted to investigate the effect of emodin on the gene expression profile changes in SCLC NCI-H446 cells.Methods NCI-H446 cells were treated with emodin and cell viability was determined by MTT assay. Cell apoptosis was determined by both flow cytometry and caspase-3 activity assay. The effect of emodin on the gene expression profile of NCI-H446 cells was analyzed using cDNA microarray. Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to validate the microarray results.Results Emodin suppressed viability, induced apoptosis and changed cell cycle of NCI-H446 cells. Among the 1262 genes, 10 genes were up-regulated and 8 genes were down-regulated more than 2 folds in NCI-H446 cells when compared with the control cells after treatment with emodin for 12 hours, while 12 genes were up-regulated and 24 genes were down-regulated after treatment with emodin for 24 hours. These genes were involved in metabolism, signal transduction, transcription regulation, cytoskeleton organization, immune response, transport, protein synthesis, cell cycle control, cell adhesion and RNA processing. The RT-PCR results were consistent with those obtained by the microarray.Conclusions Emodin affects the expression of genes involved in various cellular functions and plays important roles in cell apoptosis, tumor metastasis and chemotherapy-resistance, which suggests emodin might become an effective chemopreventive or chemotherapeutic agent for SCLC.

  17. 14 CFR 415.119 - Launch plans.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Launch plans. 415.119 Section 415.119... From a Non-Federal Launch Site § 415.119 Launch plans. An applicant's safety review document must contain the plans required by § 417.111 of this chapter, except for the countdown plan of § 417.111(l) of...

  18. China Plans To Carry Out 15 Launch Missions In 2008

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ In 2007,China made 10 launch missions and achieved complete success,including the launch of Chang'e-1 satellite,in-orbit delivery of Nigcomsat-1 and 100th launch of Long March series launch vehicle.

  19. Launch processing system concept to reality

    Science.gov (United States)

    Bailey, W. W.

    1985-01-01

    The Launch Processing System represents Kennedy Space Center's role in providing a major integrated hardware and software system for the test, checkout and launch of a new space vehicle. Past programs considered the active flight vehicle to ground interfaces as part of the flight systems and therefore the related ground system was provided by the Development Center. The major steps taken to transform the Launch Processing System from a concept to reality with the successful launches of the Shuttle Programs Space Transportation System are addressed.

  20. Launch Control System Message Reduction

    Science.gov (United States)

    Nguyen, Uyen

    2014-01-01

    System Monitoring and Control (SMC) message browsers receive many messages daily that operators do not need to see. My job is to reduce the number of messages so that warning and emergency messages can be seen easily and therefore, responded to promptly. There are two methods to reduce messages: duplicate and state-based message correlations. With duplicate message correlation, SMC display the message the first time it shows up. The next times it occurs, a duplicate number will count the number of times the message appears. State-based message correlation is a process in which more informative messages acknowledge less useful ones and send them to history. I also work on correcting the severity level and text formats of messages. I follow two SMC message filtering tenets as I'm working on this project. Firstly, before filtering an offending message, a non-conformance (NC) must be created in order to attempt fixing that message through hardware or software. Only after the NC assessment states that it cannot fix an offending message, it can be filtered by SMC. Secondly, per Launch Control System (LCS) Coding Standards, SMC does not send information messages to the active message browser unless it's a response to an operator action.

  1. 14 CFR 417.13 - Agreement with Federal launch range.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Agreement with Federal launch range. 417.13... Agreement with Federal launch range. Before conducting a licensed launch from a Federal launch range, a launch operator must— (a) Enter into an agreement with a Federal launch range to provide access to...

  2. Mini-RPV Launch System Conceptual Study

    Science.gov (United States)

    1978-12-01

    are needed to produce total launch forces sufficient to launch mini-RPV’s. 4. Votta , F. A. Jr.;THE THEORY AND DESIGN OF LONG DEFLECTION, CONSTANT...Amendment 1, 29 September 1966. 4. Votta , F. A., Jr., THE THEORY AND DESIGN OF LONG DEFLECTION, CONSTANT FORCE SPRING ELEMENTS, Transactions of the ASME

  3. China Launches Two Natural Disaster Monitoring Satellites

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ China launched two satellites, HJ-1A and HJ-1B, to monitor the environment and natural disasters at 11:25am on September 6 (Beijing time) from the Taiyuan Satellite Launch Center in Shanxi Province. The two satellites are expected to improve the country's ability in the rapid monitoring of environmental changes and reducing calamities.

  4. CHINA LAUNCHES 2 SCIENTIFIC EXPERIMENT SATELLITES

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    China placed 2 scientific experiment satellites into preset orbits atop a LM-4B launch vehicle on Sept. 9, 2004. A LM-4B blasted off at 7:14 am from Taiyuan Satellite Launch Center in Shanxi Province. Sources from the Xi'an Satellite Monitor and Control Center said that one satellite,

  5. First China-Europe Satellite Successfully Launched

    Institute of Scientific and Technical Information of China (English)

    HeYing

    2004-01-01

    On December 30, 2003 China successfully launched TC-1,the first of two scientific satellites known as Double Star, The mission,the first time that European instruments were integrated with Chinese satellites,was carried out by a Long March 2C/SM rocket at 3:06 am from the Xichang Satellite Launch Center in Sichuan province.

  6. 14 CFR 417.17 - Launch reporting requirements and launch specific updates.

    Science.gov (United States)

    2010-01-01

    ... flight safety analysis products, using previously approved methodologies, for each launch no later than...) May not change an analysis product within the final 30 days before flight unless the launch operator... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Launch reporting requirements and...

  7. 76 FR 52694 - National Environmental Policy Act: Launch of NASA Routine Payloads on Expendable Launch Vehicles

    Science.gov (United States)

    2011-08-23

    ... SPACE ADMINISTRATION National Environmental Policy Act: Launch of NASA Routine Payloads on Expendable... availability and request for comments on the draft environmental assessment (``Draft EA'') for launch of NASA routine payloads on expendable launch vehicles. SUMMARY: Pursuant to the National Environmental Policy...

  8. 14 CFR 420.29 - Launch site location review for unproven launch vehicles.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Launch site location review for unproven... AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LICENSE TO OPERATE A LAUNCH SITE Criteria and Information Requirements for Obtaining a License § 420.29 Launch site location review for...

  9. 14 CFR 420.30 - Launch site location review for permitted launch vehicles.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Launch site location review for permitted... AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LICENSE TO OPERATE A LAUNCH SITE Criteria and Information Requirements for Obtaining a License § 420.30 Launch site location review...

  10. Metformin synergistically enhances antiproliferative effects of cisplatin and etoposide in NCI-H460 human lung cancer cells

    Directory of Open Access Journals (Sweden)

    Sarah Fernandes Teixeira

    2013-12-01

    Full Text Available OBJECTIVE: To test the effectiveness of combining conventional antineoplastic drugs (cisplatin and etoposide with metformin in the treatment of non-small cell lung cancer in the NCI-H460 cell line, in order to develop new therapeutic options with high efficacy and low toxicity.METHODS: We used the 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay and calculated the combination index for the drugs studied.RESULTS: We found that the use of metformin as monotherapy reduced the metabolic viability of the cell line studied. Combining metformin with cisplatin or etoposide produced a synergistic effect and was more effective than was the use of cisplatin or etoposide as monotherapy.CONCLUSIONS: Metformin, due to its independent effects on liver kinase B1, had antiproliferative effects on the NCI-H460 cell line. When metformin was combined with cisplatin or etoposide, the cell death rate was even higher.

  11. Bisdemethoxycurcumin induces DNA damage and inhibits DNA repair associated protein expressions in NCI-H460 human lung cancer cells.

    Science.gov (United States)

    Yu, Chien-Chih; Yang, Su-Tso; Huang, Wen-Wen; Peng, Shu-Fen; Huang, An-Cheng; Tang, Nou-Ying; Liu, Hsin-Chung; Yang, Mei-Due; Lai, Kuang-Chi; Chung, Jing-Gung

    2016-12-01

    Nonsmall cell lung carcinoma (NSCLC) is a devastating primary lung tumor resistant to conventional therapies. Bisdemethoxycurcumin (BDMC) is one of curcumin derivate from Turmeric and has been shown to induce NSCLC cell death. Although there is one report to show BDMC induced DNA double strand breaks, however, no available information to show BDMC induced DNA damage action with inhibited DNA repair protein in lung cancer cells in detail. In this study, we tested BDMC-induced DNA damage and condensation in NCI-H460 cells by using Comet assay and DAPI staining examinations, respectively and we found BDMC induced DNA damage and condension. Western blotting was used to examine the effects of BDMC on protein expression associated with DNA damage and repair and results indicated that BDMC suppressed the protein levels associated with DNA damage and repair, such as 14-3-3σ (an important checkpoint keeper of DDR), O6-methylguanine-DNA methyltransferase, DNA repair proteins breast cancer 1, early onset, mediator of DNA damage checkpoint 1 but activate phosphorylated p53 and p-H2A.X (phospho Ser140) in NCI-H460 cells. Confocal laser systems microscopy was used for examining the protein translocation and results show that BDMC increased the translocation of p-p53 and p-H2A.X (phospho Ser140) from cytosol to nuclei in NCI-H460 cells. In conclusion, BDMC induced DNA damage and condension and affect DNA repair proteins in NCI-H460 cells in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1859-1868, 2016. © 2015 Wiley Periodicals, Inc.

  12. Matrine suppresses invasion and metastasis of NCI-H1299 cells by enhancing microRNA-133a expression.

    Science.gov (United States)

    Liao, Hehe; Zhao, Xixi; Qu, Jinkun; Zhang, Jia; Cai, Hui

    2015-01-01

    Matrine has been proved to inhibit proliferation and induce apoptosis of human lung cancer cells. However, less studies involved in evaluating the effects and mechanism of matrine in cell migration and invasion of lung cancer. This study was aim to investigate the involvement of miR-133a in matrine's anti-invasion and anti-metastasis in lung cancer. MTT assay was used to assess the inhibition of proliferation effects of matrine in NCI-H1299 cells. Migration and invasion abilities of NCI-H1299 cells were investigated by Transwell assays. Expression of miR-133a was detected by real-time PCR. Anti-miR technique was applied to inhibit miR-133a in matrine treated HCI-H1299 cells. Real-time PCR and Western blotting were performed to evaluate the activation of EGFR/Akt/MMP-9 pathway. As results, matrine treatment significantly inhibited proliferation, migration and invasion of NCI-H1299 cells in a concentration-dependent manner, accompanied by significantly elevation of miR-133a expression. However, matrine failed to inhibit the metastatic ability when cells transfected with anti-miR-133a. Matrine treatment also suppressed activation of EGFR/Akt/MMP-9 pathway. The inhibitory effects of matrine on activation of EGFR pathway were also reversed by anti-miR-133a transfection in NCI-H1299 cells. In conclusion, matrine inhibited the invasion and metastasis of lung cancer cell by elevating expression of miR-133a which further suppressed activation of EGFR/Akt/MMP-9 pathway.

  13. Characterizing Epistemic Uncertainty for Launch Vehicle Designs

    Science.gov (United States)

    Novack, Steven D.; Rogers, Jim; Al Hassan, Mohammad; Hark, Frank

    2016-01-01

    NASA Probabilistic Risk Assessment (PRA) has the task of estimating the aleatory (randomness) and epistemic (lack of knowledge) uncertainty of launch vehicle loss of mission and crew risk, and communicating the results. Launch vehicles are complex engineered systems designed with sophisticated subsystems that are built to work together to accomplish mission success. Some of these systems or subsystems are in the form of heritage equipment, while some have never been previously launched. For these cases, characterizing the epistemic uncertainty is of foremost importance, and it is anticipated that the epistemic uncertainty of a modified launch vehicle design versus a design of well understood heritage equipment would be greater. For reasons that will be discussed, standard uncertainty propagation methods using Monte Carlo simulation produce counter intuitive results, and significantly underestimate epistemic uncertainty for launch vehicle models. Furthermore, standard PRA methods, such as Uncertainty-Importance analyses used to identify components that are significant contributors to uncertainty, are rendered obsolete, since sensitivity to uncertainty changes are not reflected in propagation of uncertainty using Monte Carlo methods. This paper provides a basis of the uncertainty underestimation for complex systems and especially, due to nuances of launch vehicle logic, for launch vehicles. It then suggests several alternative methods for estimating uncertainty and provides examples of estimation results. Lastly, the paper describes how to implement an Uncertainty-Importance analysis using one alternative approach, describes the results, and suggests ways to reduce epistemic uncertainty by focusing on additional data or testing of selected components.

  14. Revisió dels odonats valencians de la col·lecció d’artròpodes del Museu de Ciències Naturals de Barcelona

    Directory of Open Access Journals (Sweden)

    Fontana-Bria, L.

    2012-07-01

    Full Text Available Revision of Valencian dragonflies in the arthropod collection at the Museu de Ciències Naturals de Barcelona The current work shows the species of Valencian dragonflies preserved in the arthropod collection at the Museu de Ciències Naturals de Barcelona (Spain. The collection contains a total of 33 specimens belonging to 12 species, representing 18% of the known species in the Autonomous Community of Valencia.

  15. Tabletop Experimental Track for Magnetic Launch Assist

    Science.gov (United States)

    2000-01-01

    Marshall Space Flight Center's (MSFC's) Advanced Space Transportation Program has developed the Magnetic Launch Assist System, formerly known as the Magnetic Levitation (MagLev) technology that could give a space vehicle a running start to break free from Earth's gravity. A Magnetic Launch Assist system would use magnetic fields to levitate and accelerate a vehicle along a track at speeds up to 600 mph. The vehicle would shift to rocket engines for launch into orbit. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, a Magnetic Launch Assist system would electromagnetically propel a space vehicle along the track. The tabletop experimental track for the system shown in this photograph is 44-feet long, with 22-feet of powered acceleration and 22-feet of passive braking. A 10-pound carrier with permanent magnets on its sides swiftly glides by copper coils, producing a levitation force. The track uses a linear synchronous motor, which means the track is synchronized to turn the coils on just before the carrier comes in contact with them, and off once the carrier passes. Sensors are positioned on the side of the track to determine the carrier's position so the appropriate drive coils can be energized. MSFC engineers have conducted tests on the indoor track and a 50-foot outdoor track. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  16. Magnetic Launch Assist System Demonstration Test

    Science.gov (United States)

    2001-01-01

    Engineers at the Marshall Space Flight Center (MSFC) have been testing Magnetic Launch Assist Systems, formerly known as Magnetic Levitation (MagLev) technologies. To launch spacecraft into orbit, a Magnetic Launch Assist system would use magnetic fields to levitate and accelerate a vehicle along a track at a very high speed. Similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway, the launch-assist system would electromagnetically drive a space vehicle along the track. A full-scale, operational track would be about 1.5-miles long and capable of accelerating a vehicle to 600 mph in 9.5 seconds. This photograph shows a subscale model of an airplane running on the experimental track at MSFC during the demonstration test. This track is an advanced linear induction motor. Induction motors are common in fans, power drills, and sewing machines. Instead of spinning in a circular motion to turn a shaft or gears, a linear induction motor produces thrust in a straight line. Mounted on concrete pedestals, the track is 100-feet long, about 2-feet wide, and about 1.5- feet high. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  17. A systems approach reveals distinct metabolic strategies among the NCI-60 cancer cell lines

    Science.gov (United States)

    Aurich, Maike K.; Fleming, Ronan M. T.; Thiele, Ines

    2017-01-01

    The metabolic phenotype of cancer cells is reflected by the metabolites they consume and by the byproducts they release. Here, we use quantitative, extracellular metabolomic data of the NCI-60 panel and a novel computational method to generate 120 condition-specific cancer cell line metabolic models. These condition-specific cancer models used distinct metabolic strategies to generate energy and cofactors. The analysis of the models’ capability to deal with environmental perturbations revealed three oxotypes, differing in the range of allowable oxygen uptake rates. Interestingly, models based on metabolomic profiles of melanoma cells were distinguished from other models through their low oxygen uptake rates, which were associated with a glycolytic phenotype. A subset of the melanoma cell models required reductive carboxylation. The analysis of protein and RNA expression levels from the Human Protein Atlas showed that IDH2, which was an essential gene in the melanoma models, but not IDH1 protein, was detected in normal skin cell types and melanoma. Moreover, the von Hippel-Lindau tumor suppressor (VHL) protein, whose loss is associated with non-hypoxic HIF-stabilization, reductive carboxylation, and promotion of glycolysis, was uniformly absent in melanoma. Thus, the experimental data supported the predicted role of IDH2 and the absence of VHL protein supported the glycolytic and low oxygen phenotype predicted for melanoma. Taken together, our approach of integrating extracellular metabolomic data with metabolic modeling and the combination of different network interrogation methods allowed insights into the metabolism of cells. PMID:28806730

  18. NCI Funding Trends and Priorities in Physical Activity and Energy Balance Research Among Cancer Survivors.

    Science.gov (United States)

    Alfano, Catherine M; Bluethmann, Shirley M; Tesauro, Gina; Perna, Frank; Agurs-Collins, Tanya; Elena, Joanne W; Ross, Sharon A; O'Connell, Mary; Bowles, Heather R; Greenberg, Deborah; Nebeling, Linda

    2016-01-01

    There is considerable evidence that a healthy lifestyle consisting of physical activity, healthy diet, and weight control is associated with reduced risk of morbidity and mortality after cancer. However, these behavioral interventions are not widely adopted in practice or community settings. Integrating heath behavior change interventions into standard survivorship care for the growing number of cancer survivors requires an understanding of the current state of the science and a coordinated scientific agenda for the future with focused attention in several priority areas. To facilitate this goal, this paper presents trends over the past decade of the National Cancer Institute (NCI) research portfolio, fiscal year 2004 to 2014, by funding mechanism, research focus, research design and methodology, primary study exposures and outcomes, and study team expertise and composition. These data inform a prioritized research agenda for the next decade focused on demonstrating value and feasibility and creating desire for health behavior change interventions at multiple levels including the survivor, clinician, and healthcare payer to facilitate the development and implementation of appropriately targeted, adaptive, effective, and sustainable programs for all survivors. Published by Oxford University Press (2015). This work is written by (a) US Government employee(s) and is in the public domain in the US.

  19. Pancreatitis-diabetes-pancreatic cancer: summary of an NIDDK-NCI workshop.

    Science.gov (United States)

    Andersen, Dana K; Andren-Sandberg, Åke; Duell, Eric J; Goggins, Michael; Korc, Murray; Petersen, Gloria M; Smith, Jill P; Whitcomb, David C

    2013-11-01

    A workshop sponsored by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) and the National Cancer Institute (NCI) on "Pancreatitis-Diabetes-Pancreatic Cancer" focused on the risk factors of chronic pancreatitis (CP) and diabetes mellitus (DM) on the development of pancreatic ductal adenocarcinoma (PDAC). Sessions were held on (a) an overview of the problem of PDAC; (b) CP as a risk factor of PDAC; (c) DM as a risk factor of PDAC; (d) pancreatogenic, or type 3c, DM; (e) genomic associations of CP, DM, and PDAC; (f) surveillance of high-risk populations and early detection of PDAC; and (g) effects of DM treatment on PDAC. Recent data and current understandings of the mechanisms of CP- and DM-associated factors on PDAC development were discussed, and a detailed review of the possible risks of DM treatment on the development of PDAC was provided by representatives from academia, industry, and the Food and Drug Administration. The current status of possible biomarkers of PDAC and surveillance strategies for high-risk populations were discussed, and the gaps in knowledge and opportunities for further research were elucidated. A broad spectrum of expertise of the speakers and the discussants provided an unusually productive workshop, the highlights of which are summarized in the accompanying article.

  20. Nonlinear quantitative radiation sensitivity prediction model based on NCI-60 cancer cell lines.

    Science.gov (United States)

    Zhang, Chunying; Girard, Luc; Das, Amit; Chen, Sun; Zheng, Guangqiang; Song, Kai

    2014-01-01

    We proposed a nonlinear model to perform a novel quantitative radiation sensitivity prediction. We used the NCI-60 panel, which consists of nine different cancer types, as the platform to train our model. Important radiation therapy (RT) related genes were selected by significance analysis of microarrays (SAM). Orthogonal latent variables (LVs) were then extracted by the partial least squares (PLS) method as the new compressive input variables. Finally, support vector machine (SVM) regression model was trained with these LVs to predict the SF2 (the surviving fraction of cells after a radiation dose of 2 Gy γ-ray) values of the cell lines. Comparison with the published results showed significant improvement of the new method in various ways: (a) reducing the root mean square error (RMSE) of the radiation sensitivity prediction model from 0.20 to 0.011; and (b) improving prediction accuracy from 62% to 91%. To test the predictive performance of the gene signature, three different types of cancer patient datasets were used. Survival analysis across these different types of cancer patients strongly confirmed the clinical potential utility of the signature genes as a general prognosis platform. The gene regulatory network analysis identified six hub genes that are involved in canonical cancer pathways.

  1. Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68

    Directory of Open Access Journals (Sweden)

    Bedognetti Davide

    2011-10-01

    Full Text Available Abstract Background Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. Methods In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection. Results We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection. Conclusions Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.

  2. Automatic segmentation of pulmonary nodules on CT images by use of NCI lung image database consortium

    Science.gov (United States)

    Tachibana, Rie; Kido, Shoji

    2006-03-01

    Accurate segmentation of small pulmonary nodules (SPNs) on thoracic CT images is an important technique for volumetric doubling time estimation and feature characterization for the diagnosis of SPNs. Most of the nodule segmentation algorithms that have been previously presented were designed to handle solid pulmonary nodules. However, SPNs with ground-glass opacity (GGO) also affects a diagnosis. Therefore, we have developed an automated volumetric segmentation algorithm of SPNs with GGO on thoracic CT images. This paper presents our segmentation algorithm with multiple fixed-thresholds, template-matching method, a distance-transformation method, and a watershed method. For quantitative evaluation of the performance of our algorithm, we used the first dataset provided by NCI Lung Image Database Consortium (LIDC). In the evaluation, we employed the coincident rate which was calculated with both the computerized segmented region of a SPN and the matching probability map (pmap) images provided by LIDC. As the result of 23 cases, the mean of the total coincident rate was 0.507 +/- 0.219. From these results, we concluded that our algorithm is useful for extracting SPNs with GGO and solid pattern as well as wide variety of SPNs in size.

  3. Detecting Role Errors in the Gene Hierarchy of the NCI Thesaurus

    Directory of Open Access Journals (Sweden)

    Yehoshua Perl

    2008-01-01

    Full Text Available Gene terminologies are playing an increasingly important role in the ever-growing field of genomic research. While errors in large, complex terminologies are inevitable, gene terminologies are even more susceptible to them due to the rapid growth of genomic knowledge and the nature of its discovery. It is therefore very important to establish quality- assurance protocols for such genomic-knowledge repositories. Different kinds of terminologies oftentimes require auditing methodologies adapted to their particular structures. In light of this, an auditing methodology tailored to the characteristics of the NCI Thesaurus’s (NCIT’s Gene hierarchy is presented. The Gene hierarchy is of particular interest to the NCIT’s designers due to the primary role of genomics in current cancer research. This multiphase methodology focuses on detecting role-errors, such as missing roles or roles with incorrect or incomplete target structures, occurring within that hierarchy. The methodology is based on two kinds of abstraction networks, called taxonomies, that highlight the role distribution among concepts within the IS-A (subsumption hierarchy. These abstract views tend to highlight portions of the hierarchy having a higher concentration of errors. The errors found during an application of the methodology

  4. Detecting Role Errors in the Gene Hierarchy of the NCI Thesaurus

    Science.gov (United States)

    Min, Hua; Cohen, Barry; Halper, Michael; Oren, Marc; Perl, Yehoshua

    2008-01-01

    Gene terminologies are playing an increasingly important role in the ever-growing field of genomic research. While errors in large, complex terminologies are inevitable, gene terminologies are even more susceptible to them due to the rapid growth of genomic knowledge and the nature of its discovery. It is therefore very important to establish quality-assurance protocols for such genomic-knowledge repositories. Different kinds of terminologies oftentimes require auditing methodologies adapted to their particular structures. In light of this, an auditing methodology tailored to the characteristics of the NCI Thesaurus’s (NCIT’s) Gene hierarchy is presented. The Gene hierarchy is of particular interest to the NCIT’s designers due to the primary role of genomics in current cancer research. This multiphase methodology focuses on detecting role-errors, such as missing roles or roles with incorrect or incomplete target structures, occurring within that hierarchy. The methodology is based on two kinds of abstraction networks, called taxonomies, that highlight the role distribution among concepts within the IS-A (subsumption) hierarchy. These abstract views tend to highlight portions of the hierarchy having a higher concentration of errors. The errors found during an application of the methodology are reported. Hypotheses pertaining to the efficacy of our methodology are investigated. PMID:19221606

  5. Nonlinear Quantitative Radiation Sensitivity Prediction Model Based on NCI-60 Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Chunying Zhang

    2014-01-01

    Full Text Available We proposed a nonlinear model to perform a novel quantitative radiation sensitivity prediction. We used the NCI-60 panel, which consists of nine different cancer types, as the platform to train our model. Important radiation therapy (RT related genes were selected by significance analysis of microarrays (SAM. Orthogonal latent variables (LVs were then extracted by the partial least squares (PLS method as the new compressive input variables. Finally, support vector machine (SVM regression model was trained with these LVs to predict the SF2 (the surviving fraction of cells after a radiation dose of 2 Gy γ-ray values of the cell lines. Comparison with the published results showed significant improvement of the new method in various ways: (a reducing the root mean square error (RMSE of the radiation sensitivity prediction model from 0.20 to 0.011; and (b improving prediction accuracy from 62% to 91%. To test the predictive performance of the gene signature, three different types of cancer patient datasets were used. Survival analysis across these different types of cancer patients strongly confirmed the clinical potential utility of the signature genes as a general prognosis platform. The gene regulatory network analysis identified six hub genes that are involved in canonical cancer pathways.

  6. Highlights of recent developments and trends in cancer nanotechnology research--view from NCI Alliance for Nanotechnology in Cancer.

    Science.gov (United States)

    Hull, L C; Farrell, D; Grodzinski, P

    2014-01-01

    Although the incidence of cancer and cancer related deaths in the United States has decreased over the past two decades due to improvements in early detection and treatment, cancer still is responsible for a quarter of the deaths in this country. There is much room for improvement on the standard treatments currently available and the National Cancer Institute (NCI) has recognized the potential for nanotechnology and nanomaterials in this area. The NCI Alliance for Nanotechnology in Cancer was formed in 2004 to support multidisciplinary researchers in the application of nanotechnology to cancer diagnosis and treatment. The researchers in the Alliance have been productive in generating innovative solutions to some of the central issues of cancer treatment including how to detect tumors earlier, how to target cancer cells specifically, and how to improve the therapeutic index of existing chemotherapies and radiotherapy treatments. Highly creative ideas are being pursued where novelty in nanomaterial development enables new modalities of detection or therapy. This review highlights some of the innovative materials approaches being pursued by researchers funded by the NCI Alliance. Their discoveries to improve the functionality of nanoparticles for medical applications includes the generation of new platforms, improvements in the manufacturing of nanoparticles and determining the underlying reasons for the movement of nanoparticles in the blood. © 2013.

  7. Analysis of Pathway Activity in Primary Tumors and NCI60 Cell Lines Using Gene Expression Profiling Data

    Institute of Scientific and Technical Information of China (English)

    Xing-Dong Feng; Jude E. Onyia; Shu-Yu Li; Shu-Guang Huang; Jian-Yong Shou; Bi-Rong Liao; Jonathan M. Yingling; Xiang Ye; Xi Lin; Lawrence M. Gelbert; Eric W. Su

    2007-01-01

    To determine cancer pathway activities in nine types of primary tumors and NCI60 cell lines, we applied an in silico approach by examining gene signatures reflective of consequent pathway activation using gene expression data. Supervised learning approaches predicted that the Ras pathway is active in ~70% of lung adenocarcinomas but inactive in most squamous cell carcinomas, pulmonary carcinoids, and small cell lung carcinomas. In contrast, the TGF-β, TNF-α, Src, Myc, E2F3, and β-catenin pathways are inactive in lung adenocarcinomas. We predicted an active Ras, Myc, Src, and/or E2F3 pathway in significant percentages of breast cancer, colorectal carcinoma, and gliomas. Our results also suggest that Ras may be the most prevailing oncogenic pathway. Additionally, many NCI60 cell lines exhibited a gene signature indicative of an active Ras, Myc, and/or Src, but not E2F3, β-catenin, TNF-α, or TGF-β pathway. To our knowledge, this is the first comprehensive survey of cancer pathway activities in nine major tumor types and the most widely used NCI60 cell lines. The "gene expression pathway signatures" we have defined could facilitate the understanding of molecular mechanisms in cancer development and provide guidance to the selection of appropriate cell lines for cancer research and pharmaceutical compound screening.

  8. Spergularia marina induces glucagon-like peptide-1 secretion in NCI-H716 cells through bile acid receptor activation.

    Science.gov (United States)

    Kim, Kyong; Lee, Yu Mi; Rhyu, Mee-Ra; Kim, Hye Young

    2014-11-01

    Spergularia marina Griseb. (SM) is a halophyte that grows in mud flats. The aerial portions of SM have been eaten as vegetables and traditionally used to prevent chronic diseases in Korea. However, there has been no scientific report that demonstrates the pharmacological effects of SM. Glucagon-like peptide-1 (GLP-1) is important for the maintenance of glucose and energy homeostasis through acting as a signal in peripheral and neural systems. To discover a functional food for regulating glucose and energy homeostasis, we evaluated the effect of an aqueous ethanolic extract (AEE) of SM on GLP-1 release from enteroendocrine NCI-H716 cells. In addition, we explored the Takeda G-protein-coupled receptor 5 (TGR5) agonist activity of AEE-SM in Chinese hamster ovary (CHO)-K1 cells transiently transfected with human TGR5. As a result, treatment of NCI-H716 cells with AEE-SM increased GLP-1 secretion and intracellular Ca(2+) and cyclic AMP (cAMP) levels in a dose-dependent manner. Transfection of NCI-H716 cells with TGR5-specific small interference RNA inhibited AEE-SM-induced GLP-1 secretion and the increase in Ca(2+) and cAMP levels. Moreover, AEE-SM showed that the TGR5 agonist activity in CHO-K1 cells transiently transfected with TGR5. The results suggest that AEE-SM might be a candidate for a functional food to regulate glucose and energy homeostasis.

  9. Rationale, Procedures, and Response Rates for the 2015 Administration of NCI's Health Information National Trends Survey: HINTS-FDA 2015.

    Science.gov (United States)

    Blake, Kelly D; Portnoy, David B; Kaufman, Annette R; Lin, Chung-Tung Jordan; Lo, Serena C; Backlund, Eric; Cantor, David; Hicks, Lloyd; Lin, Amy; Caporaso, Andrew; Davis, Terisa; Moser, Richard P; Hesse, Bradford W

    2016-12-01

    The National Cancer Institute (NCI) developed the Health Information National Trends Survey (HINTS) to monitor population trends in cancer communication practices, information preferences, health risk behaviors, attitudes, and cancer knowledge. The U.S. Food and Drug Administration (FDA) recognized HINTS as a unique data resource for informing its health communication endeavors and partnered with NCI to field HINTS-FDA 2015. HINTS-FDA 2015 was a self-administered paper instrument sent by mail May 29 to September 8, 2015, using a random probability-based sample of U.S. postal addresses stratified by county-level smoking rates, with an oversampling of high and medium-high smoking strata to increase the yield of current smokers responding to the survey. The response rate for HINTS-FDA 2015 was 33% (N = 3,738). The yield of current smokers (n = 495) was lower than expected, but the sampling strategy achieved the goal of obtaining more former smokers (n = 1,132). Public-use HINTS-FDA 2015 data and supporting documentation have been available for download and secondary data analyses since June 2016 at http://hints.cancer.gov . NCI and FDA encourage the use of HINTS-FDA for health communication research and practice related to tobacco-related communications, public knowledge, and behaviors as well as beliefs and actions related to medical products and dietary supplements.

  10. 78 FR 26639 - Proposed Collection; 60-Day Comment Request: Financial Sustainability of Human Tissue Biobanking...

    Science.gov (United States)

    2013-05-07

    ... HUMAN SERVICES National Institutes of Health Proposed Collection; 60-Day Comment Request: Financial..., Ph.D., Biorepositories and Biospecimen Research Branch, Cancer Diagnosis Program, 9609 Medical Center...: Financial Sustainability of Human Tissue Biobanking, 0925-NEW, National Cancer Institute (NCI),...

  11. Structural dynamics for new launch vehicles

    Science.gov (United States)

    Neighbors, Joyce; Ryan, Robert S.

    1992-01-01

    An overview is presented of current studies that will permit more robust designs and reduce the safety hazards of maximum dynamic pressure during launches. Key considerations in the assessment of future operable launch capabilities are the dynamics problems that arise during the initial minutes of transition from the static configuration on the launch pad to the attainment of orbital velocity. Attention is given to a typical attempt to achieve robustness that involves creating a design in which the first bending mode will have a high enough frequency to allow decoupling between the autopilot design and the flexible body dynamics.

  12. Les competències. La doctrina del Tribunal sobre la definició de les competències. Les competències exclusives, les compartides i les executives. - Las competencias. La doctrina del Tribunal sobre la definición de las competencias. Competencias exclusivas, compartidas y ejecutivas.

    Directory of Open Access Journals (Sweden)

    Ramon Riu

    2010-07-01

    Full Text Available La doctrina de la Sentència 31/2010 sobre la definició estatutària de les categories competencials (251-257 Mercè Barceló i SerramaleraLa doctrina del Tribunal Constitucional sobre la definició de competències. Les competències exclusives, les compartides i les executives (258-261Antoni Bayona RocamoraLa doctrina de la Sentència 31/2010 sobre les competències executives (Xavier Bernadí GilLa doctrina del Tribunal sobre la definició de les competències. Les ompetències exclusives, les compartides i les executives (270-276Marc Carrillo LópezEls efectes de la Sentència sobre la definició estatutària de les competències: la «devaluació» jurídica dels estatuts d’autonomia (277-281Mercè Corretja TorrensLes categories funcionals de competències a l’Estatut d’autonomia de Catalunya. Comentaris a la Sentència 31/2010 (282-287Ramon Riu FortunyTipologia de les competències. El seu abast funcional: els articles 110 a 112 (288-294Joaquín Tornos Massostenella e no enmendalla (262-269 La doctrina de la Sentencia 31/2010 sobre la definición estatutaria de las categorías competenciales (251-257Mercè Barceló i SerramaleraLa doctrina del Tribunal Constitucional sobre la definición de competencias. Las competencias exclusivas, las compartidas y las ejecutivas (258-261Antoni Bayona RocamoraLa doctrina de la Sentencia 31/2010 sobre las competencias ejecutivas (sostenella e no enmendalla (262-270 Xavier Bernadí GilLa doctrina del Tribunal sobre la definición de las competencias. Las competencias exclusivas, las compartidas y las ejecutivas (271-277Marc Carrillo LópezLos efectos de la Sentencia sobre la definición estatutaria de las competencias:la «devaluación» jurídica de los estatutos de autonomía (278-283Mercè Corretja TorrensLas categorías funcionales de competencias en el Estatuto de Autonomía de Cataluña. Comentarios a la Sentencia 31/2010 (284-289Ramon Riu FortunyTipología de las competencias. Su alcance

  13. Minimum Cost Nanosatellite Launch System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Delta Velocity Corporation proposes the development of a very low cost, highly responsive nanosat launch system. We propose to develop an integrated propulsion...

  14. Launch Pad Flame Trench Refractory Materials

    Science.gov (United States)

    Calle, Luz M.; Hintze, Paul E.; Parlier, Christopher R.; Bucherl, Cori; Sampson, Jeffrey W.; Curran, Jerome P.; Kolody, Mark; Perusich, Steve; Whitten, Mary

    2010-01-01

    The launch complexes at NASA's John F. Kennedy Space Center (KSC) are critical support facilities for the successful launch of space-based vehicles. These facilities include a flame trench that bisects the pad at ground level. This trench includes a flame deflector system that consists of an inverted, V-shaped steel structure covered with a high temperature concrete material five inches thick that extends across the center of the flame trench. One side of the "V11 receives and deflects the flames from the orbiter main engines; the opposite side deflects the flames from the solid rocket boosters. There are also two movable deflectors at the top of the trench to provide additional protection to shuttle hardware from the solid rocket booster flames. These facilities are over 40 years old and are experiencing constant deterioration from launch heat/blast effects and environmental exposure. The refractory material currently used in launch pad flame deflectors has become susceptible to failure, resulting in large sections of the material breaking away from the steel base structure and creating high-speed projectiles during launch. These projectiles jeopardize the safety of the launch complex, crew, and vehicle. Post launch inspections have revealed that the number and frequency of repairs, as well as the area and size of the damage, is increasing with the number of launches. The Space Shuttle Program has accepted the extensive ground processing costs for post launch repair of damaged areas and investigations of future launch related failures for the remainder of the program. There currently are no long term solutions available for Constellation Program ground operations to address the poor performance and subsequent failures of the refractory materials. Over the last three years, significant liberation of refractory material in the flame trench and fire bricks along the adjacent trench walls following Space Shuttle launches have resulted in extensive investigations of

  15. The second Ariane launch complex (ELA-2)

    Science.gov (United States)

    Dana, C.

    1985-05-01

    ELA-2 will, in 1986, become the primary Ariane launch complex, with ELA-1 being relegated to back-up roles. Both Ariane 3 and Ariane 4 vehicles can lift-off from ELA 2, but not ELA-1. In the Preparation Zone, spacecraft, launch vehicles and propellant are unloaded from shipment, stored and assembled in a one month process. The assembly building is equipped with stored ice to ensure continued air conditioning and cooling of electronic equipment and stored fuels in case of power outage. The launch gantry to which the Ariane is transported by rail is equipped with blast channels to redirect the rocket exhausts. The control center has remote cameras and sensors for monitoring launch pad activities and an underground, concrete bunker for the safety of up to 200 personnel.

  16. Launching PPARC's five year strategy programme

    CERN Multimedia

    2003-01-01

    "Over one hundred delegates from Parliament, Whitehall and Industry attended a reception on Tuesday night (25 November) to mark the launch the Particle Physics and Astronomy Research Council's (PPARC) Five Year Plan" (1 page).

  17. Metric Tracking of Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA needs reliable, accurate navigation for launch vehicles and other missions. GPS is the best world-wide navigation system, but operates at low power making it...

  18. GPS Attitude Determination for Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Toyon Research Corporation proposes to develop a family of compact, low-cost GPS-based attitude (GPS/A) sensors for launch vehicles. In order to obtain 3-D attitude...

  19. Strategies Behind The Successful Industrial Product Launch

    OpenAIRE

    Choffray, Jean-Marie; Gary L. Lilien

    1984-01-01

    In this paper, we discuss a newly-developed microcomputer decision support system useful for predicting sales growth and testing launch strategies prior to an industrial product market introduction. Peer reviewed

  20. Alstom launches new mini hydro range

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    LSTOM POWER HYDRO has announced the launch of a complete mini hydro solution. Named Mini-Aqua, the product has been developed to integrate the hydro turbine, generator and control system in a single and optimised product.

  1. National Launch System comparative economic analysis

    Science.gov (United States)

    Prince, A.

    1992-01-01

    Results are presented from an analysis of economic benefits (or losses), in the form of the life cycle cost savings, resulting from the development of the National Launch System (NLS) family of launch vehicles. The analysis was carried out by comparing various NLS-based architectures with the current Shuttle/Titan IV fleet. The basic methodology behind this NLS analysis was to develop a set of annual payload requirements for the Space Station Freedom and LEO, to design launch vehicle architectures around these requirements, and to perform life-cycle cost analyses on all of the architectures. A SEI requirement was included. Launch failure costs were estimated and combined with the relative reliability assumptions to measure the effects of losses. Based on the analysis, a Shuttle/NLS architecture evolving into a pressurized-logistics-carrier/NLS architecture appears to offer the best long-term cost benefit.

  2. Electrospun jets launched from polymeric bubbles

    Directory of Open Access Journals (Sweden)

    J.S. Varabhas

    2009-12-01

    Full Text Available In this paper the launching of liquid polymer jetsfrom the apex of gas bubbles on thepolyvinylpyrrolidone in ethanol (PVP solutionsurface due to an applied electrical potential isinvestigated. Jets of polymer launched from bubbleprovide an alternative method for electrospinningpolymer nanofibers that may be scalable forcommercial production. Bubbles were experimentallycreated on the surface of a polymer solution byforcing air through a syringe into the polymersolution. An electric potential was applied to thesolution to launch the jets. The polymer solutionconcentration was varied to determine the optimumconcentration. The semi-angle of the apex of bubblejust prior to jet launch was observed to be close to thetheoretical value of 49.3 degrees for a pendant drop.

  3. STS-114: Discovery Launch Readiness Press Conference

    Science.gov (United States)

    2005-01-01

    This press conference, attended by representatives from the national, Florida, and aerospace media, addresses launch, weather, and safety issues related to Space Shuttle Discovery prior to its launch on the STS-114 Return to Flight mission. The Master of Ceremonies is George Diller from NASA Public Affairs, and the panelists are: Space Shuttle Program Manager Bill Parsons, ISS Program Manager (JSC) Bill Gerstenmaier, Space Shuttle Deputy Program Manager Wayne Hale, Director of Shuttle Processing Mike Wetmore, ISS Program Manager (JAXA) Dr. Kuniaki Shiraki, and Launch Weather Officer (USAF) Mindy Chavez. Questions included the following topics: predicted weather conditions at launch, contingency rescue plans, countdown procedures, and risk management, as well as implications of the Return to Flight for the International Space Station (ISS).

  4. STS-114: Post Launch Press Conference

    Science.gov (United States)

    2005-01-01

    Dean Acosta, Deputy Assistant Administrator for Public Affairs hosted this post launch press conference. Present were Mike Griffin, NASA Administrator; William Ready, Associate Administrator for Space Operations; Bill Parsons, Space Shuttle Program Manager; Mike Leinbach, NASA Launch Director; and Wayne Hill, Deputy Program Manager for Space Shuttle Program. Each expressed thanks to all of NASA Officials and employees, contractors, vendors and the crew for their hard work the past two and a half years that resulted the successful and pristine launch of Space Shuttle Discovery. The Panel emphasized that through extensive technical analysis, thorough planning and tremendous amount of public support brought them full circle again to return to flight. Flight safety, debris during rocket separation, sensors, observations from the mission control, launch conditions were some of the topics discussed with the News media.

  5. NATO-3C/Delta launch

    Science.gov (United States)

    1978-01-01

    NATO-3C, the third in a series of NATO defense-related communication satellites, is scheduled to be launched on a delta vehicle from the Eastern Test Range no earlier than November 15, 1978. NATO-3A and -3B were successfully launched by Delta vehicles in April 1976 and January 1977, respectively. The NATO-3C spacecraft will be capable of transmitting voice, data, facsimile, and telex messages among military ground stations. The launch vehicle for the NATO-3C mission will be the Delta 2914 configuration. The launch vehicle is to place the spacecraft in a synchronous transfer orbit. The spacecraft Apogee Kick motor is to be fired at fifth transfer orbit apogee to circularize its orbit at geosynchronous altitude of 35,900 km(22,260 miles) above the equator over the Atlantic Ocean somewhere between 45 and 50 degrees W longitude.

  6. Visits Service Launches New Seminar Series

    CERN Multimedia

    2001-01-01

    The CERN Visits Service is launching a new series of seminars for guides, and they are open to everyone. The series kicks off next week with a talk by Konrad Elsener on the CERN neutrinos to Gran Sasso, CNGS, project.

  7. Former astronaut Armstrong witnesses STS-83 launch

    Science.gov (United States)

    1997-01-01

    Apollo l1 Commander Neil A. Armstrong and his wife, Carol, were among the many special NASA STS-83 launch guests who witnessed the liftoff of the Space Shuttle Columbia April 4 at the Banana Creek VIP Viewing Site at KSC. Columbia took off from Launch Pad 39A at 2:20:32 p.m. EST to begin the 16-day Microgravity Science Laboratory-1 (MSL-1) mission.

  8. Space Launching Site Protection against Lightning Hazards

    OpenAIRE

    Issac, F.; Bachelier, E.; Prost, D.; Enjalbert, V.; Mohedano, L.

    2012-01-01

    International audience; A launching pad, because of its activity, is particularly sensitive to the risk of lightning. The use of Standard IEC62305 "Protection against lightning" establishes the general framework for the Lightning Protection System (LPS). However, the specific activity of a launching pad requires special analysis on specific points of the LPS. Indeed, it is necessary to take into account the lightning conductor system particularity on the one hand, and the launcher electromagn...

  9. Atmospheric environment for ASTP (SA-210) launch

    Science.gov (United States)

    Johnson, D. L.

    1976-01-01

    A summary is presented of selected atmospheric conditions observed near ASTP/SA-210 launch time on July 15, 1975, at Kennedy Space Center, Florida. Values of ambient pressure, temperature, moisture, ground winds, visual observations (cloud), density, index of refraction, and wind/wind shear aloft are included. A final meteorological data tape for the ASTP launch, consisting of wind and thermodynamic parameters versus altitude, has been constructed.

  10. Comparison of Two Recent Launch Abort Platforms

    Science.gov (United States)

    Dittemore, Gary D.; Harding, Adam

    2011-01-01

    The development of new and safer manned space vehicles is a top priority at NASA. Recently two different approaches of how to accomplish this mission of keeping astronauts safe was successfully demonstrated. With work already underway on an Apollo-like launch abort system for the Orion Crew Exploration Vehicle (CEV), an alternative design concept named the Max Launch Abort System, or MLAS, was developed as a parallel effort. The Orion system, managed by the Constellation office, is based on the design of a single solid launch abort motor in a tower positioned above the capsule. The MLAS design takes a different approach placing the solid launch abort motor underneath the capsule. This effort was led by the NASA Engineering and Safety Center (NESC). Both escape systems were designed with the Ares I Rocket as the launch vehicle and had the same primary requirement to safely propel a crew module away from any emergency event either on the launch pad or during accent. Beyond these two parameters, there was little else in common between the two projects, except that they both concluded in successful launches that will further promote the development of crew launch abort systems. A comparison of these projects from the standpoint of technical requirements; program management and flight test objectives will be done to highlight the synergistic lessons learned by two engineers who worked on each program. This comparison will demonstrate how the scope of the project architecture and management involvement in innovation should be tailored to meet the specific needs of the system under development.

  11. National Security Space Launch at a Crossroads

    Science.gov (United States)

    2016-05-13

    critical” breach occurs when the program acquisition or the procurement unit cost increases 25% or more over the current baseline estimate or 50% or more...Force’s ability to continue with its current three-phase EELV acquisition strategy. These include ongoing concerns over program and launch costs ...space for national security missions. The current strategy for the EELV (Evolved Expendable Launch Vehicle) program dates from the 1990s and has since

  12. Improving global data infrastructures for more effective and scalable analysis of Earth and environmental data: the Australian NCI NERDIP Approach

    Science.gov (United States)

    Evans, Ben; Wyborn, Lesley; Druken, Kelsey; Richards, Clare; Trenham, Claire; Wang, Jingbo; Rozas Larraondo, Pablo; Steer, Adam; Smillie, Jon

    2017-04-01

    The National Computational Infrastructure (NCI) facility hosts one of Australia's largest repositories (10+ PBytes) of research data collections spanning datasets from climate, coasts, oceans, and geophysics through to astronomy, bioinformatics, and the social sciences domains. The data are obtained from national and international sources, spanning a wide range of gridded and ungridded (i.e., line surveys, point clouds) data, and raster imagery, as well as diverse coordinate reference projections and resolutions. Rather than managing these data assets as a digital library, whereby users can discover and download files to personal servers (similar to borrowing 'books' from a 'library'), NCI has built an extensive and well-integrated research data platform, the National Environmental Research Data Interoperability Platform (NERDIP, http://nci.org.au/data-collections/nerdip/). The NERDIP architecture enables programmatic access to data via standards-compliant services for high performance data analysis, and provides a flexible cloud-based environment to facilitate the next generation of transdisciplinary scientific research across all data domains. To improve use of modern scalable data infrastructures that are focused on efficient data analysis, the data organisation needs to be carefully managed including performance evaluations of projections and coordinate systems, data encoding standards and formats. A complication is that we have often found multiple domain vocabularies and ontologies are associated with equivalent datasets. It is not practical for individual dataset managers to determine which standards are best to apply to their dataset as this could impact accessibility and interoperability. Instead, they need to work with data custodians across interrelated communities and, in partnership with the data repository, the international scientific community to determine the most useful approach. For the data repository, this approach is essential to enable

  13. Deciphering causal and statistical relations of molecular aberrations and gene expressions in NCI-60 cell lines

    Directory of Open Access Journals (Sweden)

    Li Shyh-Dar

    2011-11-01

    Full Text Available Abstract Background Cancer cells harbor a large number of molecular alterations such as mutations, amplifications and deletions on DNA sequences and epigenetic changes on DNA methylations. These aberrations may dysregulate gene expressions, which in turn drive the malignancy of tumors. Deciphering the causal and statistical relations of molecular aberrations and gene expressions is critical for understanding the molecular mechanisms of clinical phenotypes. Results In this work, we proposed a computational method to reconstruct association modules containing driver aberrations, passenger mRNA or microRNA expressions, and putative regulators that mediate the effects from drivers to passengers. By applying the module-finding algorithm to the integrated datasets of NCI-60 cancer cell lines, we found that gene expressions were driven by diverse molecular aberrations including chromosomal segments' copy number variations, gene mutations and DNA methylations, microRNA expressions, and the expressions of transcription factors. In-silico validation indicated that passenger genes were enriched with the regulator binding motifs, functional categories or pathways where the drivers were involved, and co-citations with the driver/regulator genes. Moreover, 6 of 11 predicted MYB targets were down-regulated in an MYB-siRNA treated leukemia cell line. In addition, microRNA expressions were driven by distinct mechanisms from mRNA expressions. Conclusions The results provide rich mechanistic information regarding molecular aberrations and gene expressions in cancer genomes. This kind of integrative analysis will become an important tool for the diagnosis and treatment of cancer in the era of personalized medicine.

  14. Prognostic Value of Prepro-Gastrin Releasing Peptide in Lung Cancer Patients; NCI-Prospective Study

    Science.gov (United States)

    Shafik, Nevine F; Rahoma, m; Elshimy, Reham A A; M Abou El kasem, Fatma

    2016-12-01

    Background: Prior series investigated the expression of prepro-gastrin releasing peptide (prepro-GRP) in the peripheral blood of lung cancer patients. Our aim was to assess any prepro-GRP role as a prognostic factor for small cell lung cancer (SCLC) and NSCLC and correlations with clinical presentation and treatment outcome. Methods: A prospective study was conducted during the time period from the beginning of January 2012 till the end of January 2014. Prepro-GRP expression was analysed using a nested RT-PCR assay in peripheral blood of 62 untreated lung cancer patients attending the National Cancer Institute (NCI), Cairo University, and 30 age and sex matched healthy volunteers. Results: Among the 62 lung cancer cases, there were 24 (38.7%) SCLC, and 38 (61.3%) NSCLC (10 squamous cell carcinomas, 12 adenocarcinomas, 11 large cell carcinomas, 4 undifferentiated carcinomas, and 1 adenosquamous carcinoma). Twenty six patients (41.9%) were prepro-GRP positive. Prepro-GRP expression was higher (58.3%) among SCLC patients compared to NSCLC (squamous cell carcinoma (15.4%), large cell carcinoma (36.4%), and adenocarcinoma (25%)). Mean OS among prepro-GRP negative cases was longer than that among preprogastrin positive cases (17.6 vs 14.9 months). The mean PFS durations among preprogastrin negative versus positive cases were 7.7 vs 4.6 months (p= 0.041). No difference in response to chemotherapy was identified between the groups (p=0.983). Conclusion: Prepro-GRP is suggested to be a useful prognostic marker for lung cancer patients, especially with the fast- growing, bad prognostic SCLC type. More studies should aim at detailed understanding of the mechanisms of prepro-GRP action and its use in monitoring the response to treatment in a larger cohort. Creative Commons Attribution License

  15. Space Launch System for Exploration and Science

    Science.gov (United States)

    Klaus, K.

    2013-12-01

    Introduction: The Space Launch System (SLS) is the most powerful rocket ever built and provides a critical heavy-lift launch capability enabling diverse deep space missions. The exploration class vehicle launches larger payloads farther in our solar system and faster than ever before. The vehicle's 5 m to 10 m fairing allows utilization of existing systems which reduces development risks, size limitations and cost. SLS lift capacity and superior performance shortens mission travel time. Enhanced capabilities enable a myriad of missions including human exploration, planetary science, astrophysics, heliophysics, planetary defense and commercial space exploration endeavors. Human Exploration: SLS is the first heavy-lift launch vehicle capable of transporting crews beyond low Earth orbit in over four decades. Its design maximizes use of common elements and heritage hardware to provide a low-risk, affordable system that meets Orion mission requirements. SLS provides a safe and sustainable deep space pathway to Mars in support of NASA's human spaceflight mission objectives. The SLS enables the launch of large gateway elements beyond the moon. Leveraging a low-energy transfer that reduces required propellant mass, components are then brought back to a desired cislunar destination. SLS provides a significant mass margin that can be used for additional consumables or a secondary payloads. SLS lowers risks for the Asteroid Retrieval Mission by reducing mission time and improving mass margin. SLS lift capacity allows for additional propellant enabling a shorter return or the delivery of a secondary payload, such as gateway component to cislunar space. SLS enables human return to the moon. The intermediate SLS capability allows both crew and cargo to fly to translunar orbit at the same time which will simplify mission design and reduce launch costs. Science Missions: A single SLS launch to Mars will enable sample collection at multiple, geographically dispersed locations and a

  16. Strategy of Khrunichev's Launch Vehicles Further Evolution

    Science.gov (United States)

    Medvedev, A. A.; Kuzin, A. I.; Karrask, V. K.

    2002-01-01

    vehicles and it is concerned with a further evolution of its launcher fleet in order to meet arising demands of their services customers. Continuing to provide an operation of current "Proton" heavy launch vehicle and "Rockot" small launch vehicle, Khrunichev is carrying out a permanent improvement of these launchers as well as is developing new advanced launch systems. Thus, the `Proton' just has the improved "Proton-M" version, which was successfully tested in a flight, while an improvement of the "Rockot" is provided by a permanent modernization of its "Breeze-KM" upper stage and a payload fairing. Enhancing of the "Proton/Proton-M's" lift capabilities and flexibility of operation is being provided by introduction of advanced upper stages, the "Breeze- M", which was just put into service, and KVRB being in the development. "Angara-1.1" small launcher is scheduled to a launch in 2003. A creation of this family foresees not only a range of small, medium and heavy launch vehicles based on a modular principle of design but also a construction of high-automated launch site at the Russian Plesetsk spaceport. An operation of the "Angara" family's launchers will allow to inject payloads of actually all classes from Russian national territory into all range of applicable orbits with high technical and economic indices. ecological safety of drop zones, Khrunichev is developing the "Baikal" fly-back reusable booster. This booster would replace expendable first stages of small "Angaras" and strap-ons of medium/heavy launchers, which exert a most influence on the Earth's environment. intercontinental ballistic missiles to current and advanced space launch vehicles of various classes. A succession of the gained experience and found technological solutions are shown.

  17. Flight Testing of Wireless Networking for Nanosat Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The innovation proposed here addresses the testing and evaluation of wireless networking technologies for small launch vehicles by leveraging existing nanosat launch...

  18. Launch Vehicle Demonstrator Using Shuttle Assets

    Science.gov (United States)

    Threet, Grady E., Jr.; Creech, Dennis M.; Philips, Alan D.; Water, Eric D.

    2011-01-01

    The Marshall Space Flight Center Advanced Concepts Office (ACO) has the leading role for NASA s preliminary conceptual launch vehicle design and performance analysis. Over the past several years the ACO Earth-to-Orbit Team has evaluated thousands of launch vehicle concept variations for a multitude of studies including agency-wide efforts such as the Exploration Systems Architecture Study (ESAS), Constellation, Heavy Lift Launch Vehicle (HLLV), Heavy Lift Propulsion Technology (HLPT), Human Exploration Framework Team (HEFT), and Space Launch System (SLS). NASA plans to continue human space exploration and space station utilization. Launch vehicles used for heavy lift cargo and crew will be needed. One of the current leading concepts for future heavy lift capability is an inline one and a half stage concept using solid rocket boosters (SRB) and based on current Shuttle technology and elements. Potentially, the quickest and most cost-effective path towards an operational vehicle of this configuration is to make use of a demonstrator vehicle fabricated from existing shuttle assets and relying upon the existing STS launch infrastructure. Such a demonstrator would yield valuable proof-of-concept data and would provide a working test platform allowing for validated systems integration. Using shuttle hardware such as existing RS-25D engines and partial MPS, propellant tanks derived from the External Tank (ET) design and tooling, and four-segment SRB s could reduce the associated upfront development costs and schedule when compared to a concept that would rely on new propulsion technology and engine designs. There are potentially several other additional benefits to this demonstrator concept. Since a concept of this type would be based on man-rated flight proven hardware components, this demonstrator has the potential to evolve into the first iteration of heavy lift crew or cargo and serve as a baseline for block upgrades. This vehicle could also serve as a demonstration

  19. Operational Analysis in the Launch Environment

    Science.gov (United States)

    James, George; Kaouk, Mo; Cao, Tim; Fogt, Vince; Rocha, Rodney; Schultz, Ken; Tucker, Jon-Michael; Rayos, Eli; Bell,Jeff; Alldredge, David; Howsman, Tom

    2012-01-01

    The launch environment is a challenging regime to work due to changing system dynamics, changing environmental loading, joint compression loads that cannot be easily applied on the ground, and control effects. Operational testing is one of the few feasible approaches to capture system level dynamics since ground testing cannot reproduce all of these conditions easily. However, the most successful applications of Operational Modal Testing involve systems with good stationarity and long data acquisition times. This paper covers an ongoing effort to understand the launch environment and the utility of current operational modal tools. This work is expected to produce a collection of operational tools that can be applied to non-stationary launch environment, experience dealing with launch data, and an expanding database of flight parameters such as damping. This paper reports on recent efforts to build a software framework for the data processing utilizing existing and specialty tools; understand the limits of current tools; assess a wider variety of current tools; and expand the experience with additional datasets as well as to begin to address issues raised in earlier launch analysis studies.

  20. Quality function deployment in launch operations

    Science.gov (United States)

    Portanova, P. L.; Tomei, E. J., Jr.

    1990-11-01

    The goal of the Advanced Launch System (ALS) is a more efficient launch capability that provides a highly reliable and operable system at substantially lower cost than current launch systems. Total Quality Management (TQM) principles are being emphasized throughout the ALS program. A continuous improvement philosophy is directed toward satisfying users' and customer's requirements in terms of quality, performance, schedule, and cost. Quality Function Deployment (QFD) is interpreted as the voice of the customer (or user), and it is an important planning tool in translating these requirements throughout the whole process of design, development, manufacture, and operations. This report explores the application of QFD methodology to launch operations, including the modification and addition of events (operations planning) in the engineering development cycle, and presents an informal status of study results to date. QFD is a technique for systematically analyzing the customer's (Space Command) perceptions of what constitutes a highly reliable and operable system and functionally breaking down those attributes to identify the critical characteristics that determine an efficient launch system capability. In applying the principle of QFD, a series of matrices or charts are developed with emphasis on the one commonly known as the House of Quality (because of its roof-like format), which identifies and translates the most critical information.

  1. Vibro-acoustic launch protection experiment (VALPE)

    Science.gov (United States)

    Henderson, Benjamin; Gerhart, Charlotte; Lane, Steven; Jensen, Elizabeth; Griffin, Steve; Lazzaro, Anthony

    2003-10-01

    Launch acoustic and vibration loads have the potential to damage sensitive payloads within a payload fairing, often requiring more structural mass to withstand these loads than would otherwise be necessary to survive launch. Experiments demonstrating several vibro-acoustic mitigation technologies developed by AFRL/VS and its contractors flew on the Vibro-Acoustic Launch Protection Experiment 2 (VALPE-2) aboard a Terrier-Improved Orion sounding rocket from Wallops Island Flight Facility in August 2003. Flight data collected in November 2002 from a nearly identical launch (VALPE-1) was used to characterize the fairing environment for comparison. Preparations for the flight experiments are discussed along with the performance of the various experiments in flight. The several experiments include an Adaptive Vibro-Acoustic Device (AVAD) to mitigate acoustic loads, an active/passive hybrid vibration isolation system using voice-coil actuation and a ShockRing passive component, a voice-coil regenerative electronics vibration isolation system to absorb a portion of the vibration energy during launch and use it to power an active isolation system during a staging event, and a ChamberCore composite fairing with implications for passive acoustic performance.

  2. Arianespace: Launch Solutions for Scientific Endeavours

    Science.gov (United States)

    Le Gall, J.-Y.

    Arianespace was established 25 years ago and currently stands as the European operator offering launch services from the Guiana Space Centre CSG according to the mandate given by the European Member States through the European Space Agency ESA While guaranteeing access to space through commercially sustained capabilities Arianespace is first and foremost dedicated to satisfy scientific requests to orbit any mission providing reliable and available market-priced solutions to the scientific community In this regard among the 232 spacecraft deployed by Arianespace include all European space observatories such as Giotto 1985 Hipparcos 1989 ISO 1995 XMM 1999 Envisat 2002 Mars Express 2003 Rosetta 2004 Venus Express 2005 This longstanding heritage illustrates the Arianespace steady commitment toward its scientific customers to tailor launch solutions according to their requirements Arianespace operates a fleet of launch vehicles optimized for each and every scientific mission including the heavy-lift Ariane 5 the mid-size Soyuz currently launched from Baikonur and available from CSG beginning in 2008 and the light Vega starting in 2008 More than a launch Arianespace provides consummate and adapted solutions paving the way for cost-effective and target-oriented scientific endeavours

  3. NASA Facts: Nanosatellite Launch Adapter System (NLAS)

    Science.gov (United States)

    Chartres, James; Cappuccio, Gelsomina

    2013-01-01

    The Nanosatellite Launch Adapter System (NLAS) was developed to increase access to space while simplifying the integration process of miniature satellites, called nanosats or cubesats, onto launch vehicles. A standard cubesat measures about 4inches (10 cm) long, 4 inches wide,and 4 inches high, and is called a one-unit (1U) cubesat. A single NLAS provides the capability to deploy 24U of cubesats. The system is designed to accommodate satellites measuring 1U, 1.5U, 2U, 3U and 6U sizes for deployment into orbit. The NLAS may be configured for use on different launch vehicles. The system also enables flight demonstrations of new technologies in the space environment.

  4. Direct launch using the electric rail gun

    Science.gov (United States)

    Barber, J. P.

    1983-01-01

    The concept explored involves using a large single stage electric rail gun to achieve orbital velocities. Exit aerodynamics, launch package design and size, interior ballistics, system and component sizing and design concepts are treated. Technology development status and development requirements are identified and described. The expense of placing payloads in Earth orbit using conventional chemical rockets is considerable. Chemical rockets are very inefficient in converting chemical energy into payload kinetic energy. A rocket motor is relatively expensive and is usually expended on each launch. In addition specialized and expensive forms of fuel are required. Gun launching payloads directly to orbit from the Earth's surface is a possible alternative. Guns are much more energy efficient than rockets. The high capital cost of the gun installation can be recovered by reusing it over and over again. Finally, relatively inexpensive fuel and large quantities of energy are readily available to a fixed installation on the Earth's surface.

  5. Computational Environments and Analysis methods available on the NCI High Performance Computing (HPC) and High Performance Data (HPD) Platform

    Science.gov (United States)

    Evans, B. J. K.; Foster, C.; Minchin, S. A.; Pugh, T.; Lewis, A.; Wyborn, L. A.; Evans, B. J.; Uhlherr, A.

    2014-12-01

    The National Computational Infrastructure (NCI) has established a powerful in-situ computational environment to enable both high performance computing and data-intensive science across a wide spectrum of national environmental data collections - in particular climate, observational data and geoscientific assets. This paper examines 1) the computational environments that supports the modelling and data processing pipelines, 2) the analysis environments and methods to support data analysis, and 3) the progress in addressing harmonisation of the underlying data collections for future transdisciplinary research that enable accurate climate projections. NCI makes available 10+ PB major data collections from both the government and research sectors based on six themes: 1) weather, climate, and earth system science model simulations, 2) marine and earth observations, 3) geosciences, 4) terrestrial ecosystems, 5) water and hydrology, and 6) astronomy, social and biosciences. Collectively they span the lithosphere, crust, biosphere, hydrosphere, troposphere, and stratosphere. The data is largely sourced from NCI's partners (which include the custodians of many of the national scientific records), major research communities, and collaborating overseas organisations. The data is accessible within an integrated HPC-HPD environment - a 1.2 PFlop supercomputer (Raijin), a HPC class 3000 core OpenStack cloud system and several highly connected large scale and high-bandwidth Lustre filesystems. This computational environment supports a catalogue of integrated reusable software and workflows from earth system and ecosystem modelling, weather research, satellite and other observed data processing and analysis. To enable transdisciplinary research on this scale, data needs to be harmonised so that researchers can readily apply techniques and software across the corpus of data available and not be constrained to work within artificial disciplinary boundaries. Future challenges will

  6. The continuing challenge of electromagnetic launch

    Energy Technology Data Exchange (ETDEWEB)

    Cowan, M.; Cnare, E.C.; Duggin, B.W.; Kaye, R.J.; Marder, B.M.; Shokair, I.R.

    1993-07-01

    Interest in launching payloads through the atmosphere to ever higher velocity is robust. For hundreds of years, guns and rockets have been improved for this purpose until they are now considered to be near to their performance limits. While the potential of electromagnetic technology to increase launch velocity has been known since late in the nineteenth century, it was not until about 1980 that a sustained and large-scale effort was started to exploit it. Electromagnetic launcher technology is restricted here to mean only that technology which establishes both a current density, J, and a magnetic field, B, within a part of the launch package, called the armature, so that J {times} B integrated over the volume of the armature is the launching force. Research and development activity was triggered by the discovery that high velocity can be produced with a simple railgun which uses an arc for its armature. This so called ``plasma-armature railgun`` has been the launcher technology upon which nearly all of the work has focused. Still, a relatively small parallel effort has also been made to explore the potential of electromagnetic launchers which do not use sliding contacts on stationary rails to establish current in the armature. One electromagnetic launcher of this type is called an induction coilgun because armature current is established by electromagnetic induction. In this paper, we first establish terminology which we will use not only to specify requirements for successful endoatmospheric launch but also to compare different launcher types. Then, we summarize the statuses of the railgun and induction coilgun technologies and discuss the issues which must be resolved before either of these launchers can offer substantial advantage for endoatomospheric launch.

  7. Atomic hydrogen as a launch vehicle propellant

    Energy Technology Data Exchange (ETDEWEB)

    Palaszewski, B.A.

    1990-01-01

    An analysis of several atomic hydrogen launch vehicles was conducted. A discussion of the facilities and the technologies that would be needed for these vehicles is also presented. The Gross Liftoff Weights (GLOW) for two systems were estimated; their specific impulses (I{sub sp}) were 750 and 1500 lb{sub f}/s/lb{sub m}. The atomic hydrogen launch vehicles were also compared to the currently planned Advanced Launch System design concepts. Very significant GLOW reductions of 52 to 58 percent are possible over the Advanced Launch System designs. Applying atomic hydrogen propellants to upper stages was also considered. Very high I{sub sp} (greater than 750 lb{sub f}/s/lb{sub m}) is needed to enable a mass savings over advanced oxygen/hydrogen propulsion. Associated with the potential benefits of high I(sub sp) atomic hydrogen are several challenging problems. Very high magnetic fields are required to maintain the atomic hydrogen in a solid hydrogen matrix. The magnetic field strength was estimated to be 30 kilogauss (3 Tesla). Also the storage temperature of the propellant is 4 K. This very low temperature will require a large refrigeration facility for the launch vehicle. The design considerations for a very high recombination rate for the propellant are also discussed. A recombination rate of 210 cm/s is predicted for atomic hydrogen. This high recombination rate can produce very high acceleration for the launch vehicle. Unique insulation or segmentation to inhibit the propellant may be needed to reduce its recombination rate.

  8. Space Launch System Mission Flexibility Assessment

    Science.gov (United States)

    Monk, Timothy; Holladay, Jon; Sanders, Terry; Hampton, Bryan

    2012-01-01

    The Space Launch System (SLS) is envisioned as a heavy lift vehicle that will provide the foundation for future beyond low Earth orbit (LEO) missions. While multiple assessments have been performed to determine the optimal configuration for the SLS, this effort was undertaken to evaluate the flexibility of various concepts for the range of missions that may be required of this system. These mission scenarios include single launch crew and/or cargo delivery to LEO, single launch cargo delivery missions to LEO in support of multi-launch mission campaigns, and single launch beyond LEO missions. Specifically, we assessed options for the single launch beyond LEO mission scenario using a variety of in-space stages and vehicle staging criteria. This was performed to determine the most flexible (and perhaps optimal) method of designing this particular type of mission. A specific mission opportunity to the Jovian system was further assessed to determine potential solutions that may meet currently envisioned mission objectives. This application sought to significantly reduce mission cost by allowing for a direct, faster transfer from Earth to Jupiter and to determine the order-of-magnitude mass margin that would be made available from utilization of the SLS. In general, smaller, existing stages provided comparable performance to larger, new stage developments when the mission scenario allowed for optimal LEO dropoff orbits (e.g. highly elliptical staging orbits). Initial results using this method with early SLS configurations and existing Upper Stages showed the potential of capturing Lunar flyby missions as well as providing significant mass delivery to a Jupiter transfer orbit.

  9. The Prostate Cancer Biorepository Network (PCBN)

    Science.gov (United States)

    2015-10-01

    ORGANIZATION: University of Washington Seattle, WA 98195 REPORT DATE: October 2015 TYPE OF REPORT: Annual Report PREPARED FOR: U.S. Army Medical...YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE October 2015 2. REPORT TYPE Annual Report 3. DATES COVERED 30 Sep 2014 - 29 Sep 2015 4. TITLE AND...University of Washington site includes blood (serum, plasma, and buffy coat), prostatectomy tissues (frozen), biopsies and metastatic tissue from rapid

  10. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.

    Science.gov (United States)

    Mathur, Anjili; Ware, Christopher; Davis, Lenora; Gazdar, Adi; Pan, Bo-Sheng; Lutterbach, Bart

    2014-01-01

    Aberrant kinase activation resulting from mutation, amplification, or translocation can drive growth and survival in a subset of human cancer. FGFR2 is amplified in breast and gastric cancer, and we report here the first characterization of FGFR2 gene amplification in colorectal cancer in the NCI-H716 colorectal cancer cell line. FGFR2 is highly expressed and activated in NCI-H716 cells, and FGFR selective small molecule inhibitors or FGFR2 shRNA strongly inhibited cell viability in vitro, indicating "addiction" of NCI-H716 cells to FGFR2. NCI-H716 growth in a xenograft model was also inhibited by an FGFR small molecule inhibitor. FGFR2 was required for activation of multiple downstream signaling proteins including AKT, ERK, S6RP and NFKB. Inhibition of downstream kinases such as AKT or ERK alone had modest effects on proliferation, whereas combined inhibition of AKT and ERK signaling resulted in a loss of viability similar to FGFR2 inhibition. We identified elevated FGFR2 expression in a small subset of primary colorectal cancer, however FGFR2 amplification was not observed. Although FGFR2 amplification is not common in primary colon cancer or lymph node and liver metastases, other subsets of colorectal cancer such as ascites, from which the NCI-H716 cell line was derived, have yet to be tested. These results suggest that emerging FGFR inhibitor therapeutics may have efficacy in a subset of colon cancer driven by FGFR2 amplification.

  11. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.

    Directory of Open Access Journals (Sweden)

    Anjili Mathur

    Full Text Available Aberrant kinase activation resulting from mutation, amplification, or translocation can drive growth and survival in a subset of human cancer. FGFR2 is amplified in breast and gastric cancer, and we report here the first characterization of FGFR2 gene amplification in colorectal cancer in the NCI-H716 colorectal cancer cell line. FGFR2 is highly expressed and activated in NCI-H716 cells, and FGFR selective small molecule inhibitors or FGFR2 shRNA strongly inhibited cell viability in vitro, indicating "addiction" of NCI-H716 cells to FGFR2. NCI-H716 growth in a xenograft model was also inhibited by an FGFR small molecule inhibitor. FGFR2 was required for activation of multiple downstream signaling proteins including AKT, ERK, S6RP and NFKB. Inhibition of downstream kinases such as AKT or ERK alone had modest effects on proliferation, whereas combined inhibition of AKT and ERK signaling resulted in a loss of viability similar to FGFR2 inhibition. We identified elevated FGFR2 expression in a small subset of primary colorectal cancer, however FGFR2 amplification was not observed. Although FGFR2 amplification is not common in primary colon cancer or lymph node and liver metastases, other subsets of colorectal cancer such as ascites, from which the NCI-H716 cell line was derived, have yet to be tested. These results suggest that emerging FGFR inhibitor therapeutics may have efficacy in a subset of colon cancer driven by FGFR2 amplification.

  12. Launch Pad Escape System Design (Human Spaceflight)

    Science.gov (United States)

    Maloney, Kelli

    2011-01-01

    A launch pad escape system for human spaceflight is one of those things that everyone hopes they will never need but is critical for every manned space program. Since men were first put into space in the early 1960s, the need for such an Emergency Escape System (EES) has become apparent. The National Aeronautics and Space Administration (NASA) has made use of various types of these EESs over the past 50 years. Early programs, like Mercury and Gemini, did not have an official launch pad escape system. Rather, they relied on a Launch Escape System (LES) of a separate solid rocket motor attached to the manned capsule that could pull the astronauts to safety in the event of an emergency. This could only occur after hatch closure at the launch pad or during the first stage of flight. A version of a LES, now called a Launch Abort System (LAS) is still used today for all manned capsule type launch vehicles. However, this system is very limited in that it can only be used after hatch closure and it is for flight crew only. In addition, the forces necessary for the LES/LAS to get the capsule away from a rocket during the first stage of flight are quite high and can cause injury to the crew. These shortcomings led to the development of a ground based EES for the flight crew and ground support personnel as well. This way, a much less dangerous mode of egress is available for any flight or ground personnel up to a few seconds before launch. The early EESs were fairly simple, gravity-powered systems to use when thing's go bad. And things can go bad very quickly and catastrophically when dealing with a flight vehicle fueled with millions of pounds of hazardous propellant. With this in mind, early EES designers saw such a passive/unpowered system as a must for last minute escapes. This and other design requirements had to be derived for an EES, and this section will take a look at the safety design requirements had to be derived for an EES, and this section will take a look at

  13. An Overview of Advanced Concepts for Launch

    Science.gov (United States)

    2012-02-09

    Assist •Gas Dynamic Guns • Railguns Mechanical Assistance •Space Platforms and Towers •Space Elevator Breakthrough Physics Not Covered •Skyhook...30 Exemplar Status Envisioned Design Launch Assist Railguns Concept Description Pros Eval. Cons IAT-UT LCA LMS LTF nCA nMS nTF •V > 7.5km...Combining, Propagation, µW conversion. --- Gun Launch High gees, Power Sources, Aerothermal Loads. Rapid, Robust Payload Railgun High gees, Power Sources

  14. Wireless Instrumentation Use on Launch Vehicles

    Science.gov (United States)

    Sherman, Aaron

    2010-01-01

    This slide presentation reviews the results of a study on the use of wireless instrumentation and sensors on future launch vehicles. The use of wireless technologies would if feasible would allow for fewer wires, and allow for more flexibility. However, it was generally concluded that wireless solutions are not currently ready to replace wired technologies for launch vehicles. The recommendations of the study were to continue to use wired sensors as the primary choice for vehicle instrumentation, and to continue to assess needs and use wireless instrumentation where appropriate. The future work includes support efforts for wireless technologies, and continue to monitor the development of wireless solutions.

  15. Space Launch System (SLS) Mission Planner's Guide

    Science.gov (United States)

    Smith, David Alan

    2017-01-01

    The purpose of this Space Launch System (SLS) Mission Planner's Guide (MPG) is to provide future payload developers/users with sufficient insight to support preliminary SLS mission planning. Consequently, this SLS MPG is not intended to be a payload requirements document; rather, it organizes and details SLS interfaces/accommodations in a manner similar to that of current Expendable Launch Vehicle (ELV) user guides to support early feasibility assessment. Like ELV Programs, once approved to fly on SLS, specific payload requirements will be defined in unique documentation.

  16. NCI-60 whole exome sequencing and pharmacological CellMiner analyses.

    Directory of Open Access Journals (Sweden)

    William C Reinhold

    Full Text Available Exome sequencing provides unprecedented insights into cancer biology and pharmacological response. Here we assess these two parameters for the NCI-60, which is among the richest genomic and pharmacological publicly available cancer cell line databases. Homozygous genetic variants that putatively affect protein function were identified in 1,199 genes (approximately 6% of all genes. Variants that are either enriched or depleted compared to non-cancerous genomes, and thus may be influential in cancer progression and differential drug response were identified for 2,546 genes. Potential gene knockouts are made available. Assessment of cell line response to 19,940 compounds, including 110 FDA-approved drugs, reveals ≈80-fold range in resistance versus sensitivity response across cell lines. 103,422 gene variants were significantly correlated with at least one compound (at p<0.0002. These include genes of known pharmacological importance such as IGF1R, BRAF, RAD52, MTOR, STAT2 and TSC2 as well as a large number of candidate genes such as NOM1, TLL2, and XDH. We introduce two new web-based CellMiner applications that enable exploration of variant-to-compound relationships for a broad range of researchers, especially those without bioinformatics support. The first tool, "Genetic variant versus drug visualization", provides a visualization of significant correlations between drug activity-gene variant combinations. Examples are given for the known vemurafenib-BRAF, and novel ifosfamide-RAD52 pairings. The second, "Genetic variant summation" allows an assessment of cumulative genetic variations for up to 150 combined genes together; and is designed to identify the variant burden for molecular pathways or functional grouping of genes. An example of its use is provided for the EGFR-ERBB2 pathway gene variant data and the identification of correlated EGFR, ERBB2, MTOR, BRAF, MEK and ERK inhibitors. The new tools are implemented as an updated web-based Cell

  17. B-52 Launch Aircraft in Flight

    Science.gov (United States)

    2001-01-01

    NASA's venerable B-52 mothership is seen here photographed from a KC-135 Tanker aircraft. The X-43 adapter is visible attached to the right wing. The B-52, used for launching experimental aircraft and for other flight research projects, has been a familiar sight in the skies over Edwards for more than 40 years and is also both the oldest B-52 still flying and the aircraft with the lowest flight time of any B-52. NASA B-52, Tail Number 008, is an air launch carrier aircraft, 'mothership,' as well as a research aircraft platform that has been used on a variety of research projects. The aircraft, a 'B' model built in 1952 and first flown on June 11, 1955, is the oldest B-52 in flying status and has been used on some of the most significant research projects in aerospace history. Some of the significant projects supported by B-52 008 include the X-15, the lifting bodies, HiMAT (highly maneuverable aircraft technology), Pegasus, validation of parachute systems developed for the space shuttle program (solid-rocket-booster recovery system and the orbiter drag chute system), and the X-38. The B-52 served as the launch vehicle on 106 X-15 flights and flew a total of 159 captive-carry and launch missions in support of that program from June 1959 to October 1968. Information gained from the highly successful X-15 program contributed to the Mercury, Gemini, and Apollo human spaceflight programs as well as space shuttle development. Between 1966 and 1975, the B-52 served as the launch aircraft for 127 of the 144 wingless lifting body flights. In the 1970s and 1980s, the B-52 was the launch aircraft for several aircraft at what is now the Dryden Flight Research Center, Edwards, California, to study spin-stall, high-angle-of attack, and maneuvering characteristics. These included the 3/8-scale F-15/spin research vehicle (SRV), the HiMAT (Highly Maneuverable Aircraft Technology) research vehicle, and the DAST (drones for aerodynamic and structural testing). The aircraft supported

  18. Tendències en el disseny metodològic de recerca sobre l’avaluació de competències a l’educació superior

    Directory of Open Access Journals (Sweden)

    Karina Angélica Villegas Sandoval

    2017-01-01

    Full Text Available L’article té com a finalitat descriure i analitzar les metodologies d'investigació utilitzades per estudis recents que aborden el tema de l’avaluació per competències a l’educació superior i la formació docent, per tal de detectar les tendències en el disseny metodològic i orientar futurs projectes d'investigació sobre aquest tema. El mètode de treball que s'ha seguit per dur a terme aquest estudi és l’anàlisi de contingut de 22 documents trobats a Dialnet. Els resultats mostren que les investigacions que tracten el tema assenyalat han anat en augment en els últims tretze anys, i es destaca el canvi de metodologia utilitzada, amb dissenys majoritàriament descriptius i avaluatius. Al seu torn, però, crida l'atenció que un gran nombre d'estudis no expliquen ni el mètode ni el disseny d'investigació que han aplicat. Es conclou que és important que les investigacions presentin un apartat que al·ludeixi al disseny metodològic a fi d’afavorir la comprensió del lector dels processos d'indagació que s'han dut a terme.

  19. DPJ Editorial: Launching the new journal

    Directory of Open Access Journals (Sweden)

    Eugene Matusov

    2013-01-01

    Full Text Available We welcome and invite new readers, authors, reviewers and editors to the new journal.  A short history of the journal foundation is given along with the reasons for launching this publication. A long, but not finished, list is provided of important and interesting themes and areas of interest for dialogic educational practice, research and theory.

  20. Landsat Data Continuity Mission - Launch Fever

    Science.gov (United States)

    Irons, James R.; Loveland, Thomas R.; Markham, Brian L.; Masek, Jeffrey G.; Cook, Bruce; Dwyer, John L.

    2012-01-01

    The year 2013 will be an exciting period for those that study the Earth land surface from space, particularly those that observe and characterize land cover, land use, and the change of cover and use over time. Two new satellite observatories will be launched next year that will enhance capabilities for observing the global land surface. The United States plans to launch the Landsat Data Continuity Mission (LDCM) in January. That event will be followed later in the year by the European Space Agency (ESA) launch of the first Sentinel 2 satellite. Considered together, the two satellites will increase the frequency of opportunities for viewing the land surface at a scale where human impact and influence can be differentiated from natural change. Data from the two satellites will provide images for similar spectral bands and for comparable spatial resolutions with rigorous attention to calibration that will facilitate cross comparisons. This presentation will provide an overview of the LDCM satellite system and report its readiness for the January launch.

  1. Control of NASA's Space Launch System

    Science.gov (United States)

    VanZwieten, Tannen S.

    2014-01-01

    The flight control system for the NASA Space Launch System (SLS) employs a control architecture that evolved from Saturn, Shuttle & Ares I-X while also incorporating modern enhancements. This control system, baselined for the first unmanned launch, has been verified and successfully flight-tested on the Ares I-X rocket and an F/A-18 aircraft. The development of the launch vehicle itself came on the heels of the Space Shuttle retirement in 2011, and will deliver more payload to orbit and produce more thrust than any other vehicle, past or present, opening the way to new frontiers of space exploration as it carries the Orion crew vehicle, equipment, and experiments into new territories. The initial 70 metric ton vehicle consists of four RS-25 core stage engines from the Space Shuttle inventory, two 5- segment solid rocket boosters which are advanced versions of the Space Shuttle boosters, and a core stage that resembles the External Tank and carries the liquid propellant while also serving as the vehicle's structural backbone. Just above SLS' core stage is the Interim Cryogenic Propulsion Stage (ICPS), based upon the payload motor used by the Delta IV Evolved Expendable Launch Vehicle (EELV).

  2. CERF Launches CESN En Espanol in 2012

    Science.gov (United States)

    CERF launched Coastal and Estuarine Science News (CESN) in 2003 as an electronic companion to the journal Estuaries and Coasts. CESN serves to strengthen the link between science and management of coastal systems by reaching out to a wider audience with accessible summaries of 4...

  3. China Launches First Ever Nano-satellite

    Institute of Scientific and Technical Information of China (English)

    LiuJie

    2004-01-01

    China successfully launched two scientific satellites, including a nano-satellite for the first time, heralding a breakthrough in space technology. A LM-2C rocket carrying Nano-Satellite I (NS-1), which weighs just 25kg and an Experiment Satellite I, weighing 204kg blasted off at 11:59 p.m. on April 18,

  4. Gravity Probe B:. Launch and Initialization

    Science.gov (United States)

    Keiser, G. M.; Bencze, W. J.; Brumley, R. W.; Buchman, S.; Clarke, B.; Debra, D.; Everitt, C. W. F.; Green, G.; Heifetz, M. I.; Hipkins, D. N.; Holmes, T.; Li, J.; Mester, J.; Muhlfelder, B.; Murray, D.; Ohshima, Y.; Parkinson, B. W.; Salomon, M.; Santiago, D.; Shestople, P.; Silbergleit, A. S.; Solomonik, V.; Taber, M.; Turneaure, J. P.

    2005-04-01

    The scientific instrument and the major subsystems of the Gravity Probe B satellite are described. Following launch, the initial on-orbit operations were designed to check the operations of each of these major subsystems, provide an initial on-orbit calibration of the scientific instrument, set up the instrument in its operational mode, and spin up and align each of the four gyroscopes.

  5. SMAP Post-launch Field Campaign Planning

    Science.gov (United States)

    The SMAP post-launch Cal/Val activities are intended both to assess the quality of the mission products and to support analyses that lead to their improvement. A suite of complementary methodologies will be employed that will result in a robust global assessment. Much of the work will occur in the C...

  6. Air loads on solar panels during launch

    NARCIS (Netherlands)

    Beltman, W.M.; van der Hoogt, Peter; Spiering, R.M.E.J.; Tijdeman, H.

    1996-01-01

    The dynamical behaviour of solar panels during launch is significantly affected by the thin layers of air trapped between the panels. For narrow gaps the air manifests itself not only as a considerable added mass, but its viscosity can result in a substantial amount of damping. A model has been

  7. Illustration of Launching Samples Home from Mars

    Science.gov (United States)

    2005-01-01

    One crucial step in a Mars sample return mission would be to launch the collected sample away from the surface of Mars. This artist's concept depicts a Mars ascent vehicle for starting a sample of Mars rocks on their trip to Earth.

  8. CAS Launches Website for Scientific Education

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ CAS member Wang Shouguan and SecretaryGeneral of the Ministry of Science and Technology Zhang Jing'an jointly push the button on August 26 in Beijing to launch a CAS website for scientific education (http ://www.fipse. cn/).

  9. Launching a Projectile into Deep Space

    Science.gov (United States)

    Maruszewski, Richard F., Jr.

    2004-01-01

    As part of the discussion about Newton's work in a history of mathematics course, one of the presentations calculated the amount of energy necessary to send a projectile into deep space. Afterwards, the students asked for a recalculation with two changes: First the launch under study consisted of a single stage, but the students desired to…

  10. Ares Launch Vehicles Lean Practices Case Study

    Science.gov (United States)

    Doreswamy, Rajiv; Self, Timothy A.

    2007-01-01

    The Ares launch vehicles team, managed by the Ares Projects Office (APO) at NASA Marshall Space Flight Center, has completed the Ares I Crew Launch Vehicle System Requirements Review and System Definition Review and early design work for the Ares V Cargo Launch Vehicle. This paper provides examples of how Lean Manufacturing, Kaizen events, and Six Sigma practices are helping APO deliver a new space transportation capability on time and within budget, while still meeting stringent technical requirements. For example, Lean philosophies have been applied to numerous process definition efforts and existing process improvement activities, including the Ares I-X test flight Certificate of Flight Readiness (CoFR) process, risk management process, and review board organization and processes. Ares executives learned Lean practices firsthand, making the team "smart buyers" during proposal reviews and instilling the team with a sense of what is meant by "value-added" activities. Since the goal of the APO is to field launch vehicles at a reasonable cost and on an ambitious schedule, adopting Lean philosophies and practices will be crucial to the Ares Project's long-term SUCCESS.

  11. Throttleable GOX/ABS launch assist hybrid rocket motor for small scale air launch platform

    Science.gov (United States)

    Spurrier, Zachary S.

    Aircraft-based space-launch platforms allow operational flexibility and offer the potential for significant propellant savings for small-to-medium orbital payloads. The NASA Armstrong Flight Research Center's Towed Glider Air-Launch System (TGALS) is a small-scale flight research project investigating the feasibility for a remotely-piloted, towed, glider system to act as a versatile air launch platform for nano-scale satellites. Removing the crew from the launch vehicle means that the system does not have to be human rated, and offers a potential for considerable cost savings. Utah State University is developing a small throttled launch-assist system for the TGALS platform. This "stage zero" design allows the TGALS platform to achieve the required flight path angle for the launch point, a condition that the TGALS cannot achieve without external propulsion. Throttling is required in order to achieve and sustain the proper launch attitude without structurally overloading the airframe. The hybrid rocket system employs gaseous-oxygen and acrylonitrile butadiene styrene (ABS) as propellants. This thesis summarizes the development and testing campaign, and presents results from the clean-sheet design through ground-based static fire testing. Development of the closed-loop throttle control system is presented.

  12. Launching a world-class joint venture.

    Science.gov (United States)

    Bamford, James; Ernst, David; Fubini, David G

    2004-02-01

    More than 5,000 joint ventures, and many more contractual alliances, have been launched worldwide in the past five years. Companies are realizing that JVs and alliances can be lucrative vehicles for developing new products, moving into new markets, and increasing revenues. The problem is, the success rate for JVs and alliances is on a par with that for mergers and acquisitions--which is to say not very good. The authors, all McKinsey consultants, argue that JV success remains elusive for most companies because they don't pay enough attention to launch planning and execution. Most companies are highly disciplined about integrating the companies they target through M&A, but they rarely commit sufficient resources to launching similarly sized joint ventures or alliances. As a result, the parent companies experience strategic conflicts, governance gridlock, and missed operational synergies. Often, they walk away from the deal. The launch phase begins with the parent companies' signing of a memorandum of understanding and continues through the first 100 days of the JV or alliance's operation. During this period, it's critical for the parents to convene a team dedicated to exposing inherent tensions early. Specifically, the launch team must tackle four basic challenges. First, build and maintain strategic alignment across the separate corporate entities, each of which has its own goals, market pressures, and shareholders. Second, create a shared governance system for the two parent companies. Third, manage the economic interdependencies between the corporate parents and the JV. And fourth, build a cohesive, high-performing organization (the JV or alliance)--not a simple task, since most managers come from, will want to return to, and may even hold simultaneous positions in the parent companies. Using real-world examples, the authors offer their suggestions for meeting these challenges.

  13. Improving clinical research and cancer care delivery in community settings: evaluating the NCI community cancer centers program

    Directory of Open Access Journals (Sweden)

    Fennell Mary L

    2009-09-01

    Full Text Available Abstract Background In this article, we describe the National Cancer Institute (NCI Community Cancer Centers Program (NCCCP pilot and the evaluation designed to assess its role, function, and relevance to the NCI's research mission. In doing so, we describe the evolution of and rationale for the NCCCP concept, participating sites' characteristics, its multi-faceted aims to enhance clinical research and quality of care in community settings, and the role of strategic partnerships, both within and outside of the NCCCP network, in achieving program objectives. Discussion The evaluation of the NCCCP is conceptualized as a mixed method multi-layered assessment of organizational innovation and performance which includes mapping the evolution of site development as a means of understanding the inter- and intra-organizational change in the pilot, and the application of specific evaluation metrics for assessing the implementation, operations, and performance of the NCCCP pilot. The assessment of the cost of the pilot as an additional means of informing the longer-term feasibility and sustainability of the program is also discussed. Summary The NCCCP is a major systems-level set of organizational innovations to enhance clinical research and care delivery in diverse communities across the United States. Assessment of the extent to which the program achieves its aims will depend on a full understanding of how individual, organizational, and environmental factors align (or fail to align to achieve these improvements, and at what cost.

  14. Avaluació de competències professionalitzadores en els estudis de grau de comunicació audiovisual

    Directory of Open Access Journals (Sweden)

    Marina Romeo

    2017-01-01

    Full Text Available Els recents canvis en la formació universitària han comportat un destacable nivell de professionalització dels estudis i una constant adequació a les demandes socials. En aquest sentit, una de les necessitats per a la formació universitària a l'àrea de la comunicació audiovisual és desenvolupar en els estudiants competències professionalitzadores que els permetin trobar nínxols d'ocupació en un mercat altament competitiu i sotmès a canvis continus. Aquesta recerca té per objecte crear una rúbrica que permeti avaluar l'aprenentatge professionalitzador en els estudis de grau en comunicació audiovisual (CAV que es desenvolupen a Espanya. Per desenvolupar-la hem comptat amb ocupadors i acadèmics experts de l'àmbit de la comunicació audiovisual triats de forma intencional. La rúbrica final desenvolupada, a més de permetre avaluar el grau d'adquisició de les competències professionalitzadores en CAV, permet dibuixar un mapa clar de l'organització i adequació dels processos i metodologies docents. En aquest sentit, la rúbrica pot ser un instrument pedagògic clau per a una futura promoció d'estudiants, i es pot convertir en un instrument que afavoreixi l'avaluació formativa dels alumnes.

  15. Are we winning or losing the war on cancer? Deciphering the propaganda of NCI's 33-year war.

    Science.gov (United States)

    Howe, Genevieve K; Clapp, Richard W

    2004-01-01

    The National Cancer Institute (NCI) and collaborating agencies have proclaimed great progress in the U.S. "war on cancer," while at the same time presenting more reasons for concern than celebration. We reviewed various documents and data files and found that incidence and mortality rates for all cancer sites combined remain higher than they were when the "war on cancer" was declared in 1971, despite very recent, modest decreases. The burden of the disease has risen from three million to nearly ten million people. Black Americans, men of all races, and other segments of the population disproportionately bear the burden of cancer. We also looked at data for malignant breast cancer and found that incidence rates increased 36% from 1973 to 2000, while mortality for all population groups combined declined slightly. Breast cancer mortality is 34% higher among black women than among white women, even though white women are generally more likely to get the disease. The $50 billion spent on the "war on cancer" over the last 33 years has yielded few gains. The NCI's resources must be refocused on preventing cancers we know how to prevent.

  16. Comparison of the ISU, NCI, MSM, and SPADE Methods for Estimating Usual Intake: A Simulation Study of Nutrients Consumed Daily

    Directory of Open Access Journals (Sweden)

    Greice H. C. Laureano

    2016-03-01

    Full Text Available Various methods are available for estimating usual dietary intake distributions. Hence, there is a need for simulation studies to compare them. The methods Iowa State University (ISU, National Cancer Institute (NCI, Multiple Source Method (MSM and Statistical Program to Assess Dietary Exposure (SPADE were previously compared in another study, but some results were inconclusive due to the small number of replications used in the simulation. Seeking to overcome this limitation, the present study used 1000 simulated samples for 12 different scenarios to compare the accuracy of estimates yielded by the aforementioned methods. The focus is on scenarios that exhibited the most uncertainty in the conclusions of the mentioned study above, i.e., scenarios with small sample sizes, skewed intake distributions, and large ratios of the between- and within-person variances. Bias was used as a measure of accuracy. For scenarios with small sample sizes (n = 150, the ISU, MSM and SPADE methods generally achieved more accurate estimates than the NCI method, particularly for the 10th and 90th percentiles. The differences between methods became smaller with larger sample sizes (n = 300 and n = 500. With few exceptions, the methods were found to perform similarly.

  17. A Low-Cost Launch Assistance System for Orbital Launch Vehicles

    Directory of Open Access Journals (Sweden)

    Oleg Nizhnik

    2012-01-01

    Full Text Available The author reviews the state of art of nonrocket launch assistance systems (LASs for spaceflight focusing on air launch options. The author proposes an alternative technologically feasible LAS based on a combination of approaches: air launch, high-altitude balloon, and tethered LAS. Proposed LAS can be implemented with the existing off-the-shelf hardware delivering 7 kg to low-earth orbit for the 5200 USD per kg. Proposed design can deliver larger reduction in price and larger orbital payloads with the future advances in the aerostats, ropes, electrical motors, and terrestrial power networks.

  18. Mindfulness i altres tècniques per desenvolupar la intel·ligència emocional dels adolescents en contextos de consum de substàncies

    OpenAIRE

    Mañas Murcia, Rosa Mª

    2014-01-01

    Aquest treball pretén integrar els diferents treballs que s'han realitzat sobre la intel·ligència emocional i la seva correlació amb les conductes autodestructives dels adolescents, especialment les conductes de consum de substàncies. Aquesta cerca integradora de treballs es basa en la possibilitat de considerar la intel·ligència emocional (IE) com a factor de protecció, i de la seva importància de integrar-la en els programes de prevenció en addiccions de substàncies que estiguin dirigits a ...

  19. Hail Disrometer Array for Launch Systems Support

    Science.gov (United States)

    Lane, John E.; Sharp, David W.; Kasparis, Takis C.; Doesken, Nolan J.

    2008-01-01

    Prior to launch, the space shuttle might be described as a very large thermos bottle containing substantial quantities of cryogenic fuels. Because thermal insulation is a critical design requirement, the external wall of the launch vehicle fuel tank is covered with an insulating foam layer. This foam is fragile and can be damaged by very minor impacts, such as that from small- to medium-size hail, which may go unnoticed. In May 1999, hail damage to the top of the External Tank (ET) of STS-96 required a rollback from the launch pad to the Vehicle Assembly Building (VAB) for repair of the insulating foam. Because of the potential for hail damage to the ET while exposed to the weather, a vigilant hail sentry system using impact transducers was developed as a hail damage warning system and to record and quantify hail events. The Kennedy Space Center (KSC) Hail Monitor System, a joint effort of the NASA and University Affiliated Spaceport Technology Development Contract (USTDC) Physics Labs, was first deployed for operational testing in the fall of 2006. Volunteers from the Community Collaborative Rain. Hail, and Snow Network (CoCoRaHS) in conjunction with Colorado State University were and continue to be active in testing duplicate hail monitor systems at sites in the hail prone high plains of Colorado. The KSC Hail Monitor System (HMS), consisting of three stations positioned approximately 500 ft from the launch pad and forming an approximate equilateral triangle (see Figure 1), was deployed to Pad 39B for support of STS-115. Two months later, the HMS was deployed to Pad 39A for support of STS-116. During support of STS-117 in late February 2007, an unusual hail event occurred in the immediate vicinity of the exposed space shuttle and launch pad. Hail data of this event was collected by the HMS and analyzed. Support of STS-118 revealed another important application of the hail monitor system. Ground Instrumentation personnel check the hail monitors daily when a

  20. NASA's Space Launch System Progress Report

    Science.gov (United States)

    Singer, Joan A.; Cook, Jerry R.; Lyles, Garry M.; Beaman, David E.

    2011-01-01

    Exploration beyond Earth will be an enduring legacy for future generations, confirming America's commitment to explore, learn, and progress. NASA's Space Launch System (SLS) Program, managed at the Marshall Space Flight Center, is responsible for designing and developing the first exploration-class rocket since the Apollo Program's Saturn V that sent Americans to the Moon. The SLS offers a flexible design that may be configured for the MultiPurpose Crew Vehicle and associated equipment, or may be outfitted with a payload fairing that will accommodate flagship science instruments and a variety of high-priority experiments. Both options support a national capability that will pay dividends for future generations. Building on legacy systems, facilities, and expertise, the SLS will have an initial lift capability of 70 metric tons (mT) and will be evolvable to 130 mT. While commercial launch vehicle providers service the International Space Station market, this capability will surpass all vehicles, past and present, providing the means to do entirely new missions, such as human exploration of asteroids and Mars. With its superior lift capability, the SLS can expand the interplanetary highway to many possible destinations, conducting revolutionary missions that will change the way we view ourselves, our planet and its place in the cosmos. To perform missions such as these, the SLS will be the largest launch vehicle ever built. It is being designed for safety and affordability - to sustain our journey into the space age. Current plans include launching the first flight, without crew, later this decade, with crewed flights beginning early next decade. Development work now in progress is based on heritage space systems and working knowledge, allowing for a relatively quick start and for maturing the SLS rocket as future technologies become available. Together, NASA and the U.S. aerospace industry are partnering to develop this one-of-a-kind asset. Many of NASA's space

  1. FY-3A Launched Atop A LM-4C Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    Rain.L

    2008-01-01

    @@ FY-3A,the first satellite of China's new generation of polar-orbiting meteorological satellites,was launched into space atop a modified LM-4C launch vehicle.The satellite separated from the rocket 19 minutes after the takeoff.Flying at an altitude of 807km with an inclination of 98.8 degrees,the satellite circles in polar orbit 14 times everyday,covering the whole globe twice a day.

  2. Resonant mode controllers for launch vehicle applications

    Science.gov (United States)

    Schreiner, Ken E.; Roth, Mary Ellen

    Electro-mechanical actuator (EMA) systems are currently being investigated for the National Launch System (NLS) as a replacement for hydraulic actuators due to the large amount of manpower and support hardware required to maintain the hydraulic systems. EMA systems in weight sensitive applications, such as launch vehicles, have been limited to around 5 hp due to system size, controller efficiency, thermal management, and battery size. Presented here are design and test data for an EMA system that competes favorably in weight and is superior in maintainability to the hydraulic system. An EMA system uses dc power provided by a high energy density bipolar lithium thionyl chloride battery, with power conversion performed by low loss resonant topologies, and a high efficiency induction motor controlled with a high performance field oriented controller to drive a linear actuator.

  3. Integrated Entry Guidance for Reusable Launch Vehicle

    Institute of Scientific and Technical Information of China (English)

    NING Guo-dong; ZHANG Shu-guang; FANG Zhen-ping

    2007-01-01

    A method for the implementation of integrated three-degree-of-freedom constrained entry guidance for reusable launch vehicle is presented. Given any feasible entry conditions, terminal area energy management interface conditions, and the reference trajectory generated onboard then, the method can generate a longitudinal guidance profile rapidly, featuring linear quadratic regular method and a proportional-integral-derivative tracking law with time-varying gains, which satisfies all the entry corridor constraints and meets the requirements with high precision. Afterwards, by utilizing special features of crossrange parameter, establishing bank-reversal corridor,and determining bank-reversals according to dynamically adjusted method, the algorithm enables the lateral entry guidance system to fly a wide range of missions and provides reliable and good performance in the presence of significant aerodynamic modeling uncertainty.Fast trajectory guidance profiles and simulations with a reusable launch vehicle model for various missions and aerodynamic uncertainties are presented to demonstrate the capacity and reliability of this method.

  4. Spacecraft Power Source Installation at Launch Complex

    Science.gov (United States)

    Lytal, Paul; Hoffman, Pamela

    2010-01-01

    For certain space missions, an assembly must be integrated onto the spacecraft as late as possible in the launch vehicle processing flow. 12This late integration can be driven for a variety of reasons including thermal or hazardous materials constraints. This paper discusses the process of integrating an assembly onto a spacecraft as late as one week prior to the opening of the launch window. Consideration is given to achieving sufficient access for hardware integration, methods of remotely securing hardware to the spacecraft, maintaining spacecraft cleanliness throughout the integration process, and electrically integrating the component to the spacecraft. Specific examples are taken from the remote mechanical, electrical, and fluid cooling system integration of the power source onto the Mars Science Laboratory (MSL) Rover at the Atlas V Vertical Integration Facility (VIF) at Cape Canaveral Air Force Station, Florida.

  5. Offshore Space Center (offshore launch site)

    Science.gov (United States)

    Harvey, D. G.

    1980-07-01

    Any activity requiring the development of the HLLV can benefit by operations from an offshore space center (OSC) since operating near the equator provides a twenty percent increase in payload in an ecliptic plan orbit. Some OSC concepts considered include a moored floating (semisubmersible) design, a stationary design supported by fixed piles, and a combination of these two. The facility supports: a 15,000 foot long, 300 foot wide runway, designed to accommodate a two staged winged launch vehicle, with a one million pound payload capacity to low earth orbit; an industrial area for HLLV maintenance; an airport terminal, control and operation center, and observation tower; liquid hydrogen and liquid oxygen production and storage, and fuel storage platforms; a power generation station, docks with an unloading area; two separate launch sites; and living accommodations for 10,000 people. Potential sites include the Paramount Seamount in the Pacific Ocean off the north coast of South America. Cost estimates are considered.

  6. STS-114: Discovery Launch Postponement Press Briefing

    Science.gov (United States)

    2005-01-01

    This press briefing addresses the problem that occurred prior to the launch of the STS-114. Dean Acosta, Deputy Assistant Administrator of Public Affairs, introduces the panel which consists of Dr. Michael Griffin, NASA Administrator, William Readdy, Associate Administrator for Space Operations, Wayne Hale, Space Shuttle Program Deputy Manager, Steve Poulas, Orbiter Project Manager, Mike Leinbach, NASA Launch Director, and Bill Parsons, Space Shuttle Program Manager. Wayne Hale expresses that a problem occurred with one of the low level sensors in the hydrogen tank and that the cause of the problem must be identified and rectified. Steve Poulos talks about establishing a troubleshooting plan as a part of the scrub effort and Mike Leinbach describes the process of draining the external tank. Wayne Hale answers questions about the sensors and if the Space Shuttle Discovery is safe to fly and Steve Poulos answers questions about the possible suspects for this problem.

  7. Evolution of the Florida Launch Site Architecture: Embracing Multiple Customers, Enhancing Launch Opportunities

    Science.gov (United States)

    Colloredo, Scott; Gray, James A.

    2011-01-01

    The impending conclusion of the Space Shuttle Program and the Constellation Program cancellation unveiled in the FY2011 President's budget created a large void for human spaceflight capability and specifically launch activity from the Florida launch Site (FlS). This void created an opportunity to re-architect the launch site to be more accommodating to the future NASA heavy lift and commercial space industry. The goal is to evolve the heritage capabilities into a more affordable and flexible launch complex. This case study will discuss the FlS architecture evolution from the trade studies to select primary launch site locations for future customers, to improving infrastructure; promoting environmental remediation/compliance; improving offline processing, manufacturing, & recovery; developing range interface and control services with the US Air Force, and developing modernization efforts for the launch Pad, Vehicle Assembly Building, Mobile launcher, and supporting infrastructure. The architecture studies will steer how to best invest limited modernization funding from initiatives like the 21 st elSe and other potential funding.

  8. Smart Coatings for Launch Site Corrosion Protection

    Science.gov (United States)

    Calle, Luz M.

    2014-01-01

    Smart, environmentally friendly paint system for early corrosion detection, mitigation, and healing that will enable supportability in KSC launch facilities and ground systems through their operational life cycles. KSC's Corrosion Technology Laboratory is developing a smart, self-healing coating that can detect and repair corrosion at an early stage. This coating is being developed using microcapsules specifically designed to deliver the contents of their core when corrosion starts.

  9. Towards Performance Prognostics of a Launch Valve

    Science.gov (United States)

    2014-10-02

    estimations for the next step. In particular, this work implements a simplified version of the Risk-Sensitive Particle Filter (RSPF) presented by Orchard ...et. al. ( Orchard , 2010). The RSPF maintains a subset of particles in the high- risk, low-likelihood realm to maintain coverage in these areas when...This is a step towards allowing the Launch Valve performance analysis to be handled automatically in real-time onboard ship and provide timely

  10. Ares Launch Vehicles Lean Practices Case Study

    Science.gov (United States)

    Doreswamy, Rajiv, N.; Self, Timothy A.

    2008-01-01

    This viewgraph presentation describes test strategies and lean philisophies and practices that are applied to Ares Launch Vehicles. The topics include: 1) Testing strategy; 2) Lean Practices in Ares I-X; 3) Lean Practices Applied to Ares I-X Schedule; 4) Lean Event Results; 5) Lean, Six Sigma, and Kaizen Practices in the Ares Projects Office; 6) Lean and Kaizen Success Stories; and 7) Ares Six Sigma Practices.

  11. Navigation System for Reusable Launch Vehicle

    OpenAIRE

    Schlotterer, Markus

    2008-01-01

    PHOENIX is a downscaled experimental vehicle to demonstrate automatic landing capabilities of future Reusable Launch Vehicles (RLVs). PHOENIX has flown in May 2004 at NEAT (North European Aerospace Test range) in Vidsel, Sweden. As the shape of the vehicle has been designed for re-entry, the dynamics are very high and almost unstable. This requires a fast and precise GNC system. This paper describes the navigation system and the navigation filter of PHOENIX. The system is introduced and the h...

  12. White list management system was officially launched

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    March 28,in China International Exhibition Center the China National Textile and Apparel Council,held a seminar on the Japanese textile industry standards & white list management system,marking the two sides to build a white list management system was officially launched. It is understood that the white list management system developed self-regulatory standards for the Japanese textile industry,that textiles do

  13. Sinopec Launches Shanghai Asphalt Sales Company

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Sinopec Shanghai Asphalt Sales Company was launched in Shanghai on September 22, marking Sinopec as the largest asphalt supplier in China integrated in famous brand,production, sales and research, and distribution network.This is another important initiative for Sinopec's asphalt segment, after Sinopec won the bid for construction of F 1 racing course, to grasp the market opportunities, further improve the product quality and the level of after-sales services, and further make its asphalt business larger and stronger.

  14. Australia and the new reusable launch vehicles

    Science.gov (United States)

    Stalker, R. J.

    The new generation of reusable launch vehicles represented by ESA's Hermes and HOTOL, NASA's National Aerospace Plane, and the DFVLR's Saenger, promises to radically alter the economic basis of space flight by allowing such operations as the on-orbit servicing of satellites. Attention is presently drawn to the opportunities that arise for Australia's aerospace industry from the availability in Australia of two wind tunnel facilities capable of furnishing the requisite hypersonic aerothermodynamics testing capabilities for these vehicles' development.

  15. Battelle estimate of launch service demand

    Science.gov (United States)

    Conlon, R. J.; Day, J. B.

    1987-01-01

    The annual non-NASA non-DOD demand for launches by non-Soviet services is estimated for the period 1986-2001 on the basis of currently available data. The assumptions and methods employed in generating the bottom-up Outside Users Payload Model, the data sources, and the use of equivalent Space Shuttle flights as a payload measure are explained in detail. The numerical results are presented in extensive tables and graphs and briefly characterized.

  16. NASA Space Launch System Operations Strategy

    Science.gov (United States)

    Singer, Joan A.; Cook, Jerry R.

    2012-01-01

    The National Aeronautics and Space Administration's (NASA) Space Launch System (SLS) Program, managed at the Marshall Space Flight Center, is charged with delivering a new capability for human and scientific exploration beyond Earth orbit. The SLS also will provide backup crew and cargo services to the International Space Station, where astronauts have been training for long-duration voyages to destinations such as asteroids and Mars. For context, the SLS will be larger than the Saturn V, providing 10 percent more thrust at liftoff in its initial 70 metric ton (t) configuration and 20 percent more in its evolved 130 t configuration. The SLS Program knows that affordability is the key to sustainability. This paper will provide an overview of its operations strategy, which includes initiatives to reduce both development and fixed costs by using existing hardware and infrastructure assets to meet a first launch by 2017 within the projected budget. It also has a long-range plan to keep the budget flat using competitively selected advanced technologies that offer appropriate return on investment. To arrive at the launch vehicle concept, the SLS Program conducted internal engineering and business studies that have been externally validated by industry and reviewed by independent assessment panels. A series of design reference missions has informed the SLS operations concept, including launching the Orion Multi-Purpose Crew Vehicle on an autonomous demonstration mission in a lunar flyby scenario in 2017, and the first flight of a crew on Orion for a lunar flyby in 2021. Additional concepts address the processing of very large payloads, using a series of modular fairings and adapters to flexibly configure the rocket for the mission. This paper will describe how the SLS, Orion, and 21st Century Ground Systems programs are working together to create streamlined, affordable operations for sustainable exploration.

  17. Textile materials trading center formally launched online

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Textile materials trading center was formally launched online in Wuxi City,Jiangsu Province. This is the first third-party electronic trading platform for spot trading in China textile materials professional market. The project will strive to build the most influential textile materials trading center of East China,the whole country and even the whole world China textile materials trading center will be

  18. Risk Estimation Methodology for Launch Accidents.

    Energy Technology Data Exchange (ETDEWEB)

    Clayton, Daniel James; Lipinski, Ronald J.; Bechtel, Ryan D.

    2014-02-01

    As compact and light weight power sources with reliable, long lives, Radioisotope Power Systems (RPSs) have made space missions to explore the solar system possible. Due to the hazardous material that can be released during a launch accident, the potential health risk of an accident must be quantified, so that appropriate launch approval decisions can be made. One part of the risk estimation involves modeling the response of the RPS to potential accident environments. Due to the complexity of modeling the full RPS response deterministically on dynamic variables, the evaluation is performed in a stochastic manner with a Monte Carlo simulation. The potential consequences can be determined by modeling the transport of the hazardous material in the environment and in human biological pathways. The consequence analysis results are summed and weighted by appropriate likelihood values to give a collection of probabilistic results for the estimation of the potential health risk. This information is used to guide RPS designs, spacecraft designs, mission architecture, or launch procedures to potentially reduce the risk, as well as to inform decision makers of the potential health risks resulting from the use of RPSs for space missions.

  19. Illustration of Saturn V Launch Vehicle

    Science.gov (United States)

    1967-01-01

    This is a cutaway illustration of the Saturn V launch vehicle with callouts of the major components. The Saturn V is the largest and most powerful launch vehicle developed in the United States. It was a three stage rocket, 363 feet in height, used for sending American astronauts to the moon and for placing the Skylab in Earth orbit. The Saturn V was designed to perform Earth orbital missions through the use of the first two stages, while all three stages were used for lunar expeditions. The S-IC stage (first stage) was powered by five F- engines, which burned kerosene and liquid oxygen to produce more than 7,500,000 pounds of thrust. The S-II (second) stage was powered by five J-2 engines, that burned liquid hydrogen and liquid oxygen and produced 1,150,000 pounds thrust. The S-IVB (third) stage used one J-2 engine, producing 230,000 pounds of thrust, with a re-start capability. The Marshall Space Flight Center and its contractors designed, developed, and assembled the Saturn V launch vehicle stages.

  20. Lunar launch and landing facilities and operations

    Science.gov (United States)

    1987-01-01

    The Florida Institute of Technology established an Interdisciplinary Design Team to design a lunar based facility whose primary function involves launch and landing operations for future moon missions. Both manned and unmanned flight operations were considered in the study with particular design emphasis on the utilization (or reutilization) of all materials available on the moon. This resource availability includes man-made materials which might arrive in the form of expendable landing vehicles as well as in situ lunar minerals. From an engineering standpoint, all such materials are considered as to their suitability for constructing new lunar facilities and/or repairing or expanding existing structures. Also considered in this design study was a determination of the feasibility of using naturally occurring lunar materials to provide fuel components to support lunar launch operations. Conventional launch and landing operations similar to those used during the Apollo Program were investigated as well as less conventional techniques such as rail guns and electromagnetic mass drivers. The Advanced Space Design team consisted of students majoring in Physics and Space Science as well as Electrical, Mechanical, Chemical and Ocean Engineering.

  1. Globe hosts launch of new processor

    CERN Multimedia

    2006-01-01

    Launch of the quadecore processor chip at the Globe. On 14 November, in a series of major media events around the world, the chip-maker Intel launched its new 'quadcore' processor. For the regions of Europe, the Middle East and Africa, the day-long launch event took place in CERN's Globe of Science and Innovation, with over 30 journalists in attendance, coming from as far away as Johannesburg and Dubai. CERN was a significant choice for the event: the first tests of this new generation of processor in Europe had been made at CERN over the preceding months, as part of CERN openlab, a research partnership with leading IT companies such as Intel, HP and Oracle. The event also provided the opportunity for the journalists to visit ATLAS and the CERN Computer Centre. The strategy of putting multiple processor cores on the same chip, which has been pursued by Intel and other chip-makers in the last few years, represents an important departure from the more traditional improvements in the sheer speed of such chips. ...

  2. Polarization-Directed Surface Plasmon Polariton Launching

    Energy Technology Data Exchange (ETDEWEB)

    Gong, Yu; Joly, Alan G.; El-Khoury, Patrick Z.; Hess, Wayne P.

    2017-01-05

    The relative intensities of propagating surface plasmons (PSPs) simultaneously launched from opposing edges of a symmetric trench structure etched into a silver thin film may be controllably varied by tuning the linear polarization of the driving field. This is demonstrated through transient multiphoton photoemission electron microscopy measurements performed using a pair of spatially separated phase-locked femtosecond pulses. Our measurements are rationalized using finite-difference time domain simulations, which reveal that the coupling efficiency into the PSP modes is inversely proportional to the magnitude of the localized surface plasmon fields excited at the trench edges. Additional experiments on single step edges also show asymmetric PSP launching with respect to polarization, analogous to the trench results. Our combined experimental and computational results allude to the interplay between localized and propagating surface plasmon modes in the trench; strong coupling to the localized modes at the edges correlates to weak coupling to the PSP modes. Simultaneous excitation of the electric fields localized at both edges of the trench results in complex interactions between the right- and left-side PSP modes with Fabry-Perot and cylindrical modes. This results in a trench width-dependent PSP intensity ratio using otherwise identical driving fields. A systematic exploration of polarization directed PSP launching from a series of trench structures reveals an optimal PSP contrast ratio of 4.2 using a 500 nm-wide trench.

  3. NASA's Space Launch System: Development and Progress

    Science.gov (United States)

    Honeycutt, John; Lyles, Garry

    2016-01-01

    NASA is embarked on a new era of space exploration that will lead to new capabilities, new destinations, and new discoveries by both human and robotic explorers. Today, the International Space Station (ISS), supported by NASA's commercial partners, and robotic probes, are yielding knowledge that will help make this exploration possible. NASA is developing both the Orion crew vehicle and the Space Launch System (SLS) that will carry out a series of increasingly challenging missions that will eventually lead to human exploration of Mars. This paper will discuss the development and progress on the SLS. The SLS architecture was designed to be safe, affordable, and sustainable. The current configuration is the result of literally thousands of trade studies involving cost, performance, mission requirements, and other metrics. The initial configuration of SLS, designated Block 1, will launch a minimum of 70 metric tons (t) into low Earth orbit - significantly greater capability than any current launch vehicle. It is designed to evolve to a capability of 130 t through the use of upgraded main engines, advanced boosters, and a new upper stage. With more payload mass and volume capability than any rocket in history, SLS offers mission planners larger payloads, faster trip times, simpler design, shorter design cycles, and greater opportunity for mission success. Since the program was officially created in fall 2011, it has made significant progress toward first launch readiness of the Block 1 vehicle in 2018. Every major element of SLS continued to make significant progress in 2015. The Boosters element fired Qualification Motor 1 (QM-1) in March 2015, to test the 5-segment motor, including new insulation, joint, and propellant grain designs. The Stages element marked the completion of more than 70 major components of test article and flight core stage tanks. The Liquid Engines element conducted seven test firings of an RS-25 engine under SLS conditions. The Spacecraft

  4. Launch Pad Coatings for Smart Corrosion Control

    Science.gov (United States)

    Calle, Luz M.; Hintze, Paul E.; Bucherl, Cori N.; Li, Wenyan; Buhrow, Jerry W.; Curran, Jerome P.; Whitten, Mary C.

    2010-01-01

    Corrosion is the degradation of a material as a result of its interaction with the environment. The environment at the KSC launch pads has been documented by ASM International (formerly American Society for Metals) as the most corrosive in the US. The 70 tons of highly corrosive hydrochloric acid that are generated by the solid rocket boosters during a launch exacerbate the corrosiveness of the environment at the pads. Numerous failures at the pads are caused by the pitting of stainless steels, rebar corrosion, and the degradation of concrete. Corrosion control of launch pad structures relies on the use of coatings selected from the qualified products list (QPL) of the NASA Standard 5008A for Protective Coating of Carbon Steel, Stainless Steel, and Aluminum on Launch Structures, Facilities, and Ground Support Equipment. This standard was developed to establish uniform engineering practices and methods and to ensure the inclusion of essential criteria in the coating of ground support equipment (GSE) and facilities used by or for NASA. This standard is applicable to GSE and facilities that support space vehicle or payload programs or projects and to critical facilities at all NASA locations worldwide. Environmental regulation changes have dramatically reduced the production, handling, use, and availability of conventional protective coatings for application to KSC launch structures and ground support equipment. Current attrition rate of qualified KSC coatings will drastically limit the number of commercial off the shelf (COTS) products available for the Constellation Program (CxP) ground operations (GO). CxP GO identified corrosion detection and control technologies as a critical, initial capability technology need for ground processing of Ares I and Ares V to meet Constellation Architecture Requirements Document (CARD) CxP 70000 operability requirements for reduced ground processing complexity, streamlined integrated testing, and operations phase affordability

  5. Ares Launch Vehicles Overview: Space Access Society

    Science.gov (United States)

    Cook, Steve

    2007-01-01

    America is returning to the Moon in preparation for the first human footprint on Mars, guided by the U.S. Vision for Space Exploration. This presentation will discuss NASA's mission, the reasons for returning to the Moon and going to Mars, and how NASA will accomplish that mission in ways that promote leadership in space and economic expansion on the new frontier. The primary goals of the Vision for Space Exploration are to finish the International Space Station, retire the Space Shuttle, and build the new spacecraft needed to return people to the Moon and go to Mars. The Vision commits NASA and the nation to an agenda of exploration that also includes robotic exploration and technology development, while building on lessons learned over 50 years of hard-won experience. NASA is building on common hardware, shared knowledge, and unique experience derived from the Apollo Saturn, Space Shuttle, and contemporary commercial launch vehicle programs. The journeys to the Moon and Mars will require a variety of vehicles, including the Ares I Crew Launch Vehicle, which transports the Orion Crew Exploration Vehicle, and the Ares V Cargo Launch Vehicle, which transports the Lunar Surface Access Module. The architecture for the lunar missions will use one launch to ferry the crew into orbit, where it will rendezvous with the Lunar Module in the Earth Departure Stage, which will then propel the combination into lunar orbit. The imperative to explore space with the combination of astronauts and robots will be the impetus for inventions such as solar power and water and waste recycling. This next chapter in NASA's history promises to write the next chapter in American history, as well. It will require this nation to provide the talent to develop tools, machines, materials, processes, technologies, and capabilities that can benefit nearly all aspects of life on Earth. Roles and responsibilities are shared between a nationwide Government and industry team. The Exploration Launch

  6. El NCI inicia un estudio para evaluar la utilidad de la secuenciación genética para mejorar los resu

    Science.gov (United States)

    El Instituto Nacional del Cáncer (NCI) lanzará este mes un estudio clínico piloto denominado M-PACT con la finalidad de evaluar si el tratamiento asignado según mutaciones genéticas específicas puede brindar beneficios a pacientes con tumores sólidos meta

  7. Effects of Cx43 gene modification on the proliferation and migration of the human lung squamous carcinoma cell line NCI-H226.

    Science.gov (United States)

    Zang, J-P; Wei, R

    2015-10-27

    In this study, the human lung squamous carcinoma cell line NCI-H226 was transfected with the recombinant plasmid pBudCE4.1_Cx43 to explore the role of the Cx43 gene in cell growth, cell cycle, and tumor migration. pBudCE4.1-Cx43 was transfected into human lung squamous carcinoma NCI-H226 cells using Lipofectamine TM2000. The mRNA and protein expressions of Cx43 in the transfected cells were detected by reverse transcriptase polymerase chain reaction and western blot analysis. The cell-cell communication was detected using the scratch dye tracer method and the cell cycle was detected by flow cytometry. The CCK-8 proliferation, scratch healing, and cell invasion assays were performed to evaluate the effect of the Cx43 gene transfection on the proliferation, migration, and invasive abilities of NCI-H226 cells. Cx43 mRNA and protein expressions and the fluorescence intensity in the scratch healing test were significantly higher in the experimental group than those in the control and blank groups (P migration, respectively, in the experimental group, compared to the control and blank groups (P migration of human lung squamous carcinoma cell line NCI-H226, thereby inhibiting tumor cell proliferation.

  8. Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87) Cell Line

    Science.gov (United States)

    Atnip, Allison A.; Sigurdson, Gregory T.; Bomser, Joshua; Giusti, M. Mónica

    2017-01-01

    Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anthocyanins absorption may occur in the stomach, in which the acidic pH favors anthocyanin stability. A gastric epithelial cell line (NCI-N87) has been used to study the behavior of anthocyanins at a pH range of 3.0–7.4. This work examines the effects of time (0–3 h), concentration (50–1500 µM), and pH (3.0, 5.0, 7.4) on the transport and uptake of anthocyanins using NCI-N87 cells. Anthocyanins were transported from the apical to basolateral side of NCI-N87 cells in time and dose dependent manners. Over the treatment time of 3 h the rate of transport increased, especially with higher anthocyanin concentrations. The non-linear rate of transport may suggest an active mechanism for the transport of anthocyanins across the NCI-N87 monolayer. At apical pH 3.0, higher anthocyanin transport was observed compared to pH 5.0 and 7.4. Reduced transport of anthocyanins was found to occur at apical pH 5.0. PMID:28218720

  9. Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87 Cell Line

    Directory of Open Access Journals (Sweden)

    Allison A. Atnip

    2017-02-01

    Full Text Available Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anthocyanins absorption may occur in the stomach, in which the acidic pH favors anthocyanin stability. A gastric epithelial cell line (NCI-N87 has been used to study the behavior of anthocyanins at a pH range of 3.0–7.4. This work examines the effects of time (0–3 h, concentration (50–1500 µM, and pH (3.0, 5.0, 7.4 on the transport and uptake of anthocyanins using NCI-N87 cells. Anthocyanins were transported from the apical to basolateral side of NCI-N87 cells in time and dose dependent manners. Over the treatment time of 3 h the rate of transport increased, especially with higher anthocyanin concentrations. The non-linear rate of transport may suggest an active mechanism for the transport of anthocyanins across the NCI-N87 monolayer. At apical pH 3.0, higher anthocyanin transport was observed compared to pH 5.0 and 7.4. Reduced transport of anthocyanins was found to occur at apical pH 5.0.

  10. Non-small-cell lung cancer cell lines A549 and NCI-H460 express hypoxanthine guanine phosphoribosyltransferase on the plasma membrane

    Directory of Open Access Journals (Sweden)

    Townsend MH

    2017-03-01

    Full Text Available Michelle H Townsend, Michael D Anderson, Evita G Weagel, Edwin J Velazquez, K Scott Weber, Richard A Robison, Kim L O’Neill Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA Abstract: In both males and females, lung cancer is one of the most lethal cancers worldwide and accounts for >30% of cancer-related deaths. Despite advances in biomarker analysis and tumor characterization, there remains a need to find suitable biomarker antigen targets for treatment in late-stage lung cancer. Previous research on the salvage pathway enzyme TK1 shows a unique relationship with cancer patients as serum levels are raised according to cancer grade. To expand this analysis, the other salvage pathway enzymes were evaluated for possible upregulation within lung cancer. Adenine phosphoribosyltransferase, deoxycytidine kinase, and hypoxanthine guanine phosphoribosyltransferase (HPRT were assessed for their presentation on two non-small-cell lung cancer cell lines NCI-H460 and A549. In the present study, we show that deoxycytidine kinase and adenine phosphoribosyltransferase have no significant relationship with the membrane of NCI-H460 cells. However, we found significant localization of HPRT to the membrane of NCI-H460 and A549 cells. When treated with anti-HPRT antibodies, the average fluorescence of the cell population increased by 24.3% and 12.9% in NCI-H460 and A549 cells, respectively, in comparison with controls. To ensure that expression was not attributed to cytoplasmic HPRT, confocal microscopy was performed to visualize HPRT binding on the plasma membrane. After staining NCI-H460 cells treated with both fluorescent antibodies and a membrane-specific dye, we observed direct overlap between HPRT and the membrane of the cancer cells. Additionally, gold-conjugated antibodies were used to label and quantify the amount of HPRT on the cell surface using scanning electron microscopy and energy-dispersive analysis X

  11. Integration of GC/EI-MS and GC/NCI-MS for simultaneous quantitative determination of opiates, amphetamines, MDMA, ketamine, and metabolites in human hair.

    Science.gov (United States)

    Wu, Ya-Hsueh; Lin, Keh-Liang; Chen, Su-Chin; Chang, Yan-Zin

    2008-07-15

    In this paper, the possibility of using a multiple ionization mode approach of GC/MS was developed for the simultaneous hair testing of common drugs of abuse in Asia, including amphetamines (amphetamine, AP; methamphetamine, MA; methylenedioxy amphetamine, MDA; methylenedioxy methamphetamine, MDMA; methylenedioxy ethylamphetamine, MDEA), ketamine (ketamine, K; norketamine, NK), and opiates (morphine, MOR; codeine, COD; 6-acetylmorphine, 6-AM). This strategy integrated the characteristics of gas chromatography-mass spectrometry (GC-MS) using electron impact ionization (EI) and negative chemical ionization (NCI). Hair samples (25 mg) were washed, cut, and incubated overnight at 25 degrees C in methanol-trifluoroacetic acid (methanol-TFA). The samples were extracted by solid phase extraction (SPE) procedure, derivatized using heptafluorobutyric acid anhydride (HFBA) at 70 degrees C for 30 min, and the derivatives analyzed by GC-MS with EI and NCI. The limit of detection (LOD) with GC/EI-MS analysis obtained were 0.03 ng/mg for AP, MA, MDA, MDMA, and MDEA; 0.05 ng/mg for K, NK, MOR, and COD; and 0.08 ng/mg for 6-AM. The LOD of GC/NCI-MS analysis was much lower than GC/EI-MS analysis. The LOD obtained were 30 pg/mg for AP and MDA in GC/EI-MS and 2 pg/mg in GC/NCI-MS. Therefore, the sensitivity of AP and MDA in GC/NCI-MS was improved from 15-fold compared with EI. The sensitivity of AP, MA, MDA, MDMA, MDEA, MOR, and COD was improved from 15- to 60-fold compared with EI. In addition, the sensitivity of 6-AM increased 8-fold through selection of m/z 197 for the quantitative ion. Moreover, K and NK could dramatically improve their sensitivity at 200- and 2000-fold. The integration of GC/EI-MS and GC/NCI-MS can obtain the high sensitivity and complementary results of drugs of abuse in hair. Six hair samples from known drug abusers were examined by this new strategy. These results show that integrating the characteristics of GC/EI-MS and GC/NCI-MS were not only enhancement of

  12. Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87

    Institute of Scientific and Technical Information of China (English)

    Xiao-Chao Wei; Xin-Juan Wang; Kai-Chen; Lei Zhang; Yu Liang; Xin-Li Lin

    2001-01-01

    AIM To clone the cDNA fragment of human TRAIL (TNFrelated apoptosis inducing ligand) into a tetracyclineregulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCl-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells. METHODS The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracyclineresponsive element (TRE) to obtain the plasmid pRevTRETRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet- On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87.The resulting cell line NCI-N87-Tet-On-TRE-TRAIL and a control cell line, NCI-N87-Tet-On-TRE, were established.TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction. RESULTS The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67TetOn were obtained, with titers of about 108CFU@ L-1 By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On-TRETRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCIN87. When Dox was added, cell death was obvious in the test groups (29% -77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium. CONCLUSION With the use of the

  13. La ciència ficció : un pont entre les ciències i les Humanidades

    OpenAIRE

    2006-01-01

    La hipòtesi vol contestar la pregunta següent: pot la ciència-ficció ajudar a construir un nexe d'unió afavorint una lectura ètica de la ciència i la tecnologia? Esbrinant els motius dels distanciament entre ciències i Humanidades, veient en particular com és tractada l'ètica en la literatura de ciència-ficció i cercant punts de connexió entre ambdós mons. La hipótesis pretende contestar la siguiente pregunta: ¿puede la ciencia-ficción ayudar a construir un nexo de unión favoreciendo una l...

  14. Using CellMiner 1.6 for Systems Pharmacology and Genomic Analysis of the NCI-60

    Science.gov (United States)

    Reinhold, William C.; Sunshine, Margot; Varma, Sudhir; Doroshow, James H.; Pommier, Yves

    2015-01-01

    The NCI-60 cancer cell line panel provides a premier model for data integration and systems pharmacology being the largest publicly available database of anticancer drug activity,, genomic, molecular, and phenotypic data. It comprises gene expression (25,722 transcripts), microRNAs (360 miRNAs), whole genome DNA copy number (23,413 genes), whole exome sequencing (variants for 16,568 genes), protein levels (94 genes), and cytotoxic activity (20,861 compounds). Included are 158 Food and Drug Administration (FDA)-approved drugs and 79 that are in clinical trials. To improve data accessibility to bioinformaticists and non-bioinformaticists alike, we have developed the CellMiner web-based tools. Here we describe the newest CellMiner version, including integration of novel databases and tools associated with whole exome sequencing and protein expression, and review the tools. Included are i) “Cell line signature” for DNA, RNA, protein and drugs, ii) “Cross correlations” for up to 150 input genes, microRNAs, and compounds in a single query, iii) “Pattern comparison” to identify connections among drugs, gene expression, genomic variants, microRNA and protein expressions, iv) “Genetic variation versus drug visualization” to identify potential new drug:gene DNA variant relationships, and v) “Genetic variant summation”, designed to provide a synopsis of mutational burden on any pathway or gene group for up to 150 genes. Together, these tools allow users to flexibly query the NCI-60 data for potential relationships between genomic, molecular and pharmacological parameters in a manner specific to the user’s area of expertise. Examples for both gain- (RAS) and loss- (PTEN) of-function alterations are provided. PMID:26048278

  15. Gene expression profiles of the NCI-60 human tumor cell lines define molecular interaction networks governing cell migration processes.

    Directory of Open Access Journals (Sweden)

    Kurt W Kohn

    Full Text Available Although there is extensive information on gene expression and molecular interactions in various cell types, integrating those data in a functionally coherent manner remains challenging. This study explores the premise that genes whose expression at the mRNA level is correlated over diverse cell lines are likely to function together in a network of molecular interactions. We previously derived expression-correlated gene clusters from the database of the NCI-60 human tumor cell lines and associated each cluster with function categories of the Gene Ontology (GO database. From a cluster rich in genes associated with GO categories related to cell migration, we extracted 15 genes that were highly cross-correlated; prominent among them were RRAS, AXL, ADAM9, FN14, and integrin-beta1. We then used those 15 genes as bait to identify other correlated genes in the NCI-60 database. A survey of current literature disclosed, not only that many of the expression-correlated genes engaged in molecular interactions related to migration, invasion, and metastasis, but that highly cross-correlated subsets of those genes engaged in specific cell migration processes. We assembled this information in molecular interaction maps (MIMs that depict networks governing 3 cell migration processes: degradation of extracellular matrix, production of transient focal complexes at the leading edge of the cell, and retraction of the rear part of the cell. Also depicted are interactions controlling the release and effects of calcium ions, which may regulate migration in a spaciotemporal manner in the cell. The MIMs and associated text comprise a detailed and integrated summary of what is currently known or surmised about the role of the expression cross-correlated genes in molecular networks governing those processes.

  16. Is a mechanism such as the NCI's Clinical Alert ever an appropriate alternative to journal peer review?

    Science.gov (United States)

    DeVita, V T

    1991-01-01

    Responsibly handled, a mechanism such as a Clinical Alert can provide physicians with enough information to deal with the early release of data to the public. The journal peer review system was established primarily as an academic tool to allow publication of scientific work for the purpose of communicating information among scientists and physicians. Since both the number of papers published by an investigator and the quality of the journal publishing them are important to academic advancement, bypassing this system naturally threatens many physicians and scientists. The Clinical Alert, however, served its purpose and was well received by the majority of practicing physicians and the public. In fact, a second Alert was issued by NCI in October 1989 when data became available on the effectiveness of a new adjuvant drug therapy for a common stage of colon cancer (Appendix B) and, at a workshop convened by the National Cancer Advisory Board, where the mechanism of the Clinical Alerts was discussed, it was apparent that most participants felt that maintaining this process was important. In light of the current revolution in biology, the public's interest in receiving the benefits of its financial support of the national research enterprise, and access by the press to both scientific literature and meetings, it seems appropriate for public officials, especially when mandated by Congress, to take the responsibility of releasing some types of data to the public before traditional peer review. Some visible public decision-making process must, however, be used where the pros and cons of early release of a particular data set can be discussed, as was done with the original NCI Clinical Alert.(ABSTRACT TRUNCATED AT 250 WORDS)

  17. Constellation Ground Systems Launch Availability Analysis: Enhancing Highly Reliable Launch Systems Design

    Science.gov (United States)

    Gernand, Jeffrey L.; Gillespie, Amanda M.; Monaghan, Mark W.; Cummings, Nicholas H.

    2010-01-01

    Success of the Constellation Program's lunar architecture requires successfully launching two vehicles, Ares I/Orion and Ares V/Altair, in a very limited time period. The reliability and maintainability of flight vehicles and ground systems must deliver a high probability of successfully launching the second vehicle in order to avoid wasting the on-orbit asset launched by the first vehicle. The Ground Operations Project determined which ground subsystems had the potential to affect the probability of the second launch and allocated quantitative availability requirements to these subsystems. The Ground Operations Project also developed a methodology to estimate subsystem reliability, availability and maintainability to ensure that ground subsystems complied with allocated launch availability and maintainability requirements. The verification analysis developed quantitative estimates of subsystem availability based on design documentation; testing results, and other information. Where appropriate, actual performance history was used for legacy subsystems or comparative components that will support Constellation. The results of the verification analysis will be used to verify compliance with requirements and to highlight design or performance shortcomings for further decision-making. This case study will discuss the subsystem requirements allocation process, describe the ground systems methodology for completing quantitative reliability, availability and maintainability analysis, and present findings and observation based on analysis leading to the Ground Systems Preliminary Design Review milestone.

  18. Flight Record Of Long March Series Of Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Ying

    2008-01-01

    @@ (Continued) The 13th Launch On February 1, 1986, a LM-3 launch vehicle sent DFH-2, the 3rd geosynehronous experimental communications satellite of China, into space. The satellite entered the preset orbit.

  19. Mary Tyler Moore Helps Launch NIH MedlinePlus Magazine

    Science.gov (United States)

    ... Issues Mary Tyler Moore Helps Launch NIH MedlinePlus Magazine Past Issues / Winter 2007 Table of Contents For ... Javascript on. Among those attending the NIH MedlinePlus magazine launch on Capitol Hill were (l-r) NIH ...

  20. Lidar measurements of launch vehicle exhaust plumes

    Science.gov (United States)

    Dao, Phan D.; Curtis, David; Farley, Robert; Soletsky, Philip; Davidson, Gilbert; Gelbwachs, Jerry A.

    1997-10-01

    The Mobile Lidar Trailer (MLT) was developed and operated to characterize launch vehicle exhaust plume and its effects on the environment. Two recent applications of this facility are discussed in this paper. In the first application, the MLT was used to characterize plumes in the stratosphere up to 45 km in support of the Air Force Space and Missile Center's Rocket Impact on Stratospheric Ozone program. Solid rocket motors used by Titan IV and other heavy launch vehicles release large quantities of gaseous hydrochloric acid in the exhaust and cause concerns about a possible depletion of the ozone layer. The MLT was deployed to Cape Canaveral Air Station since October 1995 to monitor ozone and to investigate plume dynamics and properties. Six campaigns have been conducted and more are planned to provide unique data with the objective of addressing the environmental issues. The plume was observed to disperse rapidly into horizontally extended yet surprisingly thin layer with thickness recorded in over 700 lidar profiles to be less than 250 meters. MLT operates with the laser wavelengths of 532, 355 and 308 nm and a scanning receiving telescope. Data on particle backscattering at the three wavelengths suggest a consistent growth of particle size in the 2-3 hour observation sessions following the launch. In the second type of application, the MLT was used as a remote sensor of nitrogen dioxide, a caustic gaseous by-product of common liquid propellant oxidizer. Two campaigns were conducted at the Sol Se Mete Canyon test site in New Mexico in December 1996 an January 1997 to study the dispersion of nitrogen dioxide and rocket plume.

  1. Structural Weight Estimation for Launch Vehicles

    Science.gov (United States)

    Cerro, Jeff; Martinovic, Zoran; Su, Philip; Eldred, Lloyd

    2002-01-01

    This paper describes some of the work in progress to develop automated structural weight estimation procedures within the Vehicle Analysis Branch (VAB) of the NASA Langley Research Center. One task of the VAB is to perform system studies at the conceptual and early preliminary design stages on launch vehicles and in-space transportation systems. Some examples of these studies for Earth to Orbit (ETO) systems are the Future Space Transportation System [1], Orbit On Demand Vehicle [2], Venture Star [3], and the Personnel Rescue Vehicle[4]. Structural weight calculation for launch vehicle studies can exist on several levels of fidelity. Typically historically based weight equations are used in a vehicle sizing program. Many of the studies in the vehicle analysis branch have been enhanced in terms of structural weight fraction prediction by utilizing some level of off-line structural analysis to incorporate material property, load intensity, and configuration effects which may not be captured by the historical weight equations. Modification of Mass Estimating Relationships (MER's) to assess design and technology impacts on vehicle performance are necessary to prioritize design and technology development decisions. Modern CAD/CAE software, ever increasing computational power and platform independent computer programming languages such as JAVA provide new means to create greater depth of analysis tools which can be included into the conceptual design phase of launch vehicle development. Commercial framework computing environments provide easy to program techniques which coordinate and implement the flow of data in a distributed heterogeneous computing environment. It is the intent of this paper to present a process in development at NASA LaRC for enhanced structural weight estimation using this state of the art computational power.

  2. The study on vitro effect of Pokemon expression suppression in human lung cancer cell line NCI-H520%抑制Pokemon蛋白表达对人肺癌细胞株NCI-H520的作用

    Institute of Scientific and Technical Information of China (English)

    袁坦; 姜国忠; 李宏云

    2014-01-01

    目的 利用RNA干扰(RNAi)技术将针对性构建的重组质粒转染入人肺癌细胞株NCI-H520,观察下调Pokemon基因对人肺癌细胞生物学行为的影响.方法 利用Ambion公司设计软件设计针对Pokemon基因的2条小干扰RNA(siRNA)序列,并合成2对互补的siRNA编码DNA片段,定向克隆至RNAi-Ready pSIREN-RetroQ载体的BamH Ⅰ和EcoR Ⅰ位点,构建重组体质粒,分别命名为pSIREN-Pokemon-1和pSIREN-Pokemon-2,同时设立转染空载体对照组.Western blot检测人肺癌细胞中Pokemon蛋白表达,噻唑蓝(MTT)比色实验检测转染后细胞的增殖抑制结果,流式细胞分析技术检测转染后细胞周期的分布.结果 利用RNAi-Ready pSIRENR RetroQ载体构建的重组质粒可以成功抑制人肺癌细胞NCI-H520中Pokemon蛋白的表达.Western blot检测发现人肺癌细胞NCI-H520中Pokemon蛋白高水平表达,转染后其表达水平明显下降.转染后第3天,MTT比色实验显示pSIREN-Pokemon-1组(0.001 5 ±0.003 0)和pSIREN-Pokemon-2组(0.0086±0.0015)与pSIREN-Pokemon-0组(0.023 0 ±0.001 0)比较差异有统计学意义(P<0.05),而pSIREN-Pokemon-1与pSIREN-Pokemon-2组之间差异无统计学意义(P>0.05).流式细胞分析检测发现转染siRNA细胞与转染空载体细胞的细胞周期差异无统计学意义(P>0.05).pSIREN-Pokemon-1转染组及pSIREN-Pokemon-2转染组形成克隆的数量(18.0±0.3、16.0±0.4)明显低于pSIREN-Pokemon-0组(52.0±0.4,P<0.05).结论 使用pSIREN载体成功构建的重组质粒转染人肺癌细胞可有效抑制原癌基因Pokemon的表达.转染后细胞增殖受到抑制,生长速度减慢.抑制Pokemon蛋白表达对细胞周期无明显影响.靶向siRNA可以在人肺癌细胞中抑制Pokemon蛋白表达,负调节肺癌细胞生长.%Objective The aim of this research was to observe the influence of the low expression of Pokemom gene to biological behaviour of human lung cancer cell after transfecting the targeted recombinant plasmid

  3. Asiasat launch aboard Long March 3 from Xichang, China

    Science.gov (United States)

    Maack, Lou

    The history of the flight of Asiasat on April 7, 1990 aboard the Long March 3 launch vehicle from Xichang, China, is briefly reviewed. The discussion focuses on the spacecraft, the launch vehicle, and the on-site launch campaign. The discussion of the launch campaign includes the facilities at Xichang, the Hughes on-site organization, technology transfer prevention, transportation and logistics, and telecommunications.

  4. CNPC Launching Major Restructuring for Higher Efficiency

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    @@ China National Petroleum Corporation (CNPC) launched a major restructuring of the onshore oil industry in late November 1996 to accelerate the transition to a market economy. The onshore oil industry, created under a centrally planned system, has been for decades under one management operating within one budget. In accordance with market economy rules and international conventions,this restructuring is aimed at separating oil exploration and development from technical services and logistics, hospitals and schools and all other non-oil businesses, thus making the oil industry more efficient and flexible.

  5. NASA to launch second business communications satellite

    Science.gov (United States)

    1981-01-01

    The two stage Delta 3910 launch vehicle was chosen to place the second small business satellite (SBS-B) into a transfer orbit with an apogee of 36,619 kilometers and a perigee of 167 km, at an inclination of 27.7 degrees to Earth's equator. The firing and separation sequence and the inertial guidance system are described as well as the payload assist module. Facilities and services for tracking and control by NASA, COMSAT, Intelsat, and SBS are outlined and prelaunch operations are summarized.

  6. Grenade-launched imaging projectile system (GLIMPS)

    Science.gov (United States)

    Nunan, Scott C.; Coakley, Peter G.; Niederhaus, Gregory A.; Lum, Chris

    2001-09-01

    A system has been developed for delivering and attaching a sensor payload to a target using a standard 40-mm grenade launcher. The projectile incorporates an attachment mechanism, a shock mitigation system, a power source, and a video-bandwidth transmitter. Impact and launch g-loads have been limited to less than 10,000 g's, enabling sensor payloads to be assembled using Commercial Off-The-Shelf components. The GLIMPS projectile is intended to be a general-purpose delivery system for a variety of sensor payloads under the Unattended Ground Sensors program. Test results and development issues are presented.

  7. Post-launch operations and data production

    Science.gov (United States)

    Beech, P.; Meyer, D.

    1990-01-01

    Postlaunch operations scheduled in connection with the October 5, 1990, launch of Space Shuttle Discovery with the Ulysses payload are detailed. A summary is presented of postlaunch events up to and including the separation of Ulysses from Discovery, providing a timeline for the upper-stage burn and spacecraft separation. A timeline is also provided for the payload switch-on. Initial in-orbit and routine operations are discussed and missions-operations organization, the data ground segment, and Ulysses data production are considered.

  8. ICAD- A tool for decision making launched

    CSIR Research Space (South Africa)

    Murambadoro, M

    2013-05-01

    Full Text Available Affairs, Rev Thivhilaeli Nedohe, the launch was attended by Absa officials, sponsors Powerade and Coca- Cola, Student Representative Council President Andisani Mathelemusa, student affairs staff members and students. “A sustainable culture of sport... the country for the benefit of all of its people and not just of a minority. “The work universities do is vital to ensuring that we develop citizens who are able to live in and contribute to a productive, well-functioning and relatively contented society...

  9. China Returning to International Commercial Launch Service Market

    Institute of Scientific and Technical Information of China (English)

    SunQing

    2005-01-01

    China launched its first commercial mission after 6 years since July 1999. APStar 6, the communications satellite manufactured by Alcatel Space, lifted off from Xichang Satellite Launch Center and was put into preset orbit by the LM-3B launch vehicle on the evening of April 12, 2005.

  10. Fight Record Of Long March Series Of Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Ying

    2008-01-01

    @@ On June 1,2007,China launched SinoSat-3,a communications satellite,onboard a Long March(LM)-3A launch vehicle,marking the 100th flight of the Long March series of launch vehicles and the 58th consecutive success since October 1996 (at the end of 2007,the number of consecutive successes was further increased to 62).

  11. Innovative Manufacturing of Launch Vehicle Structures - Integrally Stiffened Cylinder Process

    Science.gov (United States)

    Wagner, John; Domack, Marcia; Tayon, Wesley; Bird, Richard K.

    2017-01-01

    Reducing launch costs is essential to ensuring the success of NASA's visions for planetary exploration and earth science, economical support of the International Space Station, and competitiveness of the U.S. commercial launch industry. Reducing launch vehicle manufacturing cost supports NASA's budget and technology development priorities.

  12. Launch Pad 39 Hail Monitor Array System

    Science.gov (United States)

    2008-01-01

    Weather conditions at Kennedy Space Center are extremely dynamic, and they greatly affect the safety of the Space Shuttles sitting on the launch pads. For example, on May 13, 1999, the foam on the External Tank (ET) of STS-96 was significantly damaged by hail at the launch pad, requiring rollback to the Vehicle Assembly Building. The loss of ET foam on STS-114 in 2005 intensified interest in monitoring and measuring damage to ET foam, especially from hail. But hail can be difficult to detect and monitor because it is often localized and obscured by heavy rain. Furthermore, the hot Florida climate usually melts the hail even before the rainfall subsides. In response, the hail monitor array (HMA) system, a joint effort of the Applied Physics Laboratory operated by NASA and ASRC Aerospace at KSC, was deployed for operational testing in the fall of 2006. Volunteers from the Community Collaborative Rain, Hail, and Snow (CoCoRaHS) network, in conjunction with Colorado State University, continue to test duplicate hail monitor systems deployed in the high plains of Colorado.

  13. Parametric Testing of Launch Vehicle FDDR Models

    Science.gov (United States)

    Schumann, Johann; Bajwa, Anupa; Berg, Peter; Thirumalainambi, Rajkumar

    2011-01-01

    For the safe operation of a complex system like a (manned) launch vehicle, real-time information about the state of the system and potential faults is extremely important. The on-board FDDR (Failure Detection, Diagnostics, and Response) system is a software system to detect and identify failures, provide real-time diagnostics, and to initiate fault recovery and mitigation. The ERIS (Evaluation of Rocket Integrated Subsystems) failure simulation is a unified Matlab/Simulink model of the Ares I Launch Vehicle with modular, hierarchical subsystems and components. With this model, the nominal flight performance characteristics can be studied. Additionally, failures can be injected to see their effects on vehicle state and on vehicle behavior. A comprehensive test and analysis of such a complicated model is virtually impossible. In this paper, we will describe, how parametric testing (PT) can be used to support testing and analysis of the ERIS failure simulation. PT uses a combination of Monte Carlo techniques with n-factor combinatorial exploration to generate a small, yet comprehensive set of parameters for the test runs. For the analysis of the high-dimensional simulation data, we are using multivariate clustering to automatically find structure in this high-dimensional data space. Our tools can generate detailed HTML reports that facilitate the analysis.

  14. Illustration of Ares I During Launch

    Science.gov (United States)

    2006-01-01

    The NASA developed Ares rockets, named for the Greek god associated with Mars, will return humans to the moon and later take them to Mars and other destinations. In this early illustration, the Ares I is illustrated during lift off. Ares I is an inline, two-stage rocket configuration topped by the Orion crew vehicle and its launch abort system. With a primary mission of carrying four to six member crews to Earth orbit, Ares I may also use its 25-ton payload capacity to deliver resources and supplies to the International Space Station (ISS), or to 'park' payloads in orbit for retrieval by other spacecraft bound for the moon or other destinations. Ares I uses a single five-segment solid rocket booster, a derivative of the space shuttle solid rocket booster, for the first stage. A liquid oxygen/liquid hydrogen J-2X engine, derived from the J-2 engine used on the second stage of the Apollo vehicle, will power the Ares I second stage. Ares I can lift more than 55,000 pounds to low Earth orbit. The Ares I is subject to configuration changes before it is actually launched. This illustration reflects the latest configuration as of September 2006.

  15. Contraception. Low-dose pill launched.

    Science.gov (United States)

    1993-01-01

    At a vibrant ceremony in Kampala, Uganda, the Minister of Women in Development, Youth and Culture launched the new low-dose oral contraceptive Pilplan which provides women more options for birth spacing. Diplomats, physicians, government officials, and business leaders attended the ceremony at the Sheraton Hotel Kampala. A dance group did an interpretation of "Women in Uganda: Gaining Momentum." The Minister considered the introduction of this new pill as a turning point for reproductive rights. A baseline survey among Ugandan women has shown that although almost all women were familiar with the pill, only 36% have ever used it and only 15% were currently using it. 80% thought that pill use was preferable to having an unplanned pregnancy. These findings convinced the Minister that ignorance and misconception keep women from using the pill. The government, health providers, and others need to educate women about Pilplan and how to use it correctly. A bilateral agreement between the Ministry of Health and USAID set in motion a social marketing project which has now launched two contraceptive methods: Pilplan in 1993 and the Protector condom in 1990. USAID vowed to continue to support Pilplan, particularly if men could also help in supporting birth spacing. A Uganda-based pharmaceutical firm will distribute Pilplan in Uganda through pharmacies, clinics, and health facilities. Pilplan targets all middle- to low-income women.

  16. Launched electrons in plasma opening switches

    Science.gov (United States)

    Mendel, C. W., Jr.; Rochau, G. E.; Sweeney, M. A.; McDaniel, D. H.; Quintenz, J. P.; Savage, M. E.; Lindman, E. L.; Kindel, J. M.

    Plasma opening switches have provided a means to improve the characteristics of super-power pulse generators. Recent advances involving plasma control with fast and slow magnetic fields have made these switches more versatile, allowing for improved switch uniformity, triggering, and opening current levels that are set by the level of auxiliary fields. Such switches necessarily involve breaks in the translational symmetry of the transmission line geometry and therefore affect the electron flow characteristics of the line. These symmetry breaks are the result of high electric field regions caused by plasma conductors remaining in the transmission line, ion beams crossing the line, or auxilliary magnetic field regions. Symmetry breaks cause the canonical momentum of the electrons to change, thereby moving them away from the cathode. Additional electrons are pulled from the cathode into the magnetically insulated flow, resulting in an excess of electron flow over that expected for the voltage and line current downstream of the switch. These electrons are called launched electrons. Unless they are recaptured at the cathode or else are fed into the load and used beneficially, they cause a large power loss downstream. Examples are shown of SuperMite and PBFA II data showing these losses, the tools used to study them are explained, and the mechanisms employed to mitigate the problem are discussed. The losses will be reduced primarily by reducing the amount of launched electron flow.

  17. The launch of new-look Chishango.

    Science.gov (United States)

    Chavasse, D

    2002-09-01

    PSI/Malawi is a local affiliate of the non-profit NGO, Population Services International, which operates in over 50 countries worldwide. PSI/Malawi's mission is to "improve and sustain the health of all Malawians through cost-effective social marketing of needed and affordable health products". In this context, social marketing involves using a range of media channels to create demand for branded health products which are sold at subsidised prices through a wide range of distribution outlets (e.g. wholesalers/retailers, institutions, NGOs, the workplace, etc.). Chishango is PSI/Malawi's condom brand which was launched in 1994 to provide sexually active Malawians with an affordable means of protecting themselves and their partners from HIV transmission. In 2001, research indicated that the brand needed a 'face lift' to improve its relevance to modern Malawians and therefore lead to an increase in consistent condom use resulting in a further reduction in HIV transmission. The newly packaged and positioned Chishango was launched on the 13th May 2002. The speech below was given by the Resident Director of PSI/Malawi, Dr Desmond Chavasse at the relaunch of Chishango.

  18. Solid Rocket Launch Vehicle Explosion Environments

    Science.gov (United States)

    Richardson, E. H.; Blackwood, J. M.; Hays, M. J.; Skinner, T.

    2014-01-01

    Empirical explosion data from full scale solid rocket launch vehicle accidents and tests were collected from all available literature from the 1950s to the present. In general data included peak blast overpressure, blast impulse, fragment size, fragment speed, and fragment dispersion. Most propellants were 1.1 explosives but a few were 1.3. Oftentimes the data from a single accident was disjointed and/or missing key aspects. Despite this fact, once the data as a whole was digitized, categorized, and plotted clear trends appeared. Particular emphasis was placed on tests or accidents that would be applicable to scenarios from which a crew might need to escape. Therefore, such tests where a large quantity of high explosive was used to initiate the solid rocket explosion were differentiated. Also, high speed ground impacts or tests used to simulate such were also culled. It was found that the explosions from all accidents and applicable tests could be described using only the pressurized gas energy stored in the chamber at the time of failure. Additionally, fragmentation trends were produced. Only one accident mentioned the elusive "small" propellant fragments, but upon further analysis it was found that these were most likely produced as secondary fragments when larger primary fragments impacted the ground. Finally, a brief discussion of how this data is used in a new launch vehicle explosion model for improving crew/payload survival is presented.

  19. Evolved Expendable Launch Vehicle: DOD Is Assessing Data on Worldwide Launch Market to Inform New Acquisition Strategy

    Science.gov (United States)

    2016-07-22

    Worldwide Launch Market to Inform New Acquisition Strategy Dear Mr. Chairman: This report formally transmits the information we provided in a briefing on...efforts to incorporate consideration of the global launch market into the next Evolved Expendable Launch Vehicle (EELV) program acquisition strategy . The...current and predicted military, civil and commercial launch markets into its acquisition strategy , we obtained information from Air Force and DOD

  20. FGF-2对小细胞肺癌细胞株NCI-H446 survivin表达及Smac亚细胞定位的影响%Effect of FGF-2 on survivin and subcellular location of Smac in human small cell lung cancer cell NCI-H446

    Institute of Scientific and Technical Information of China (English)

    肖德胜; 曾慧秋; 邓征浩; 屈晓辉; 文继舫; 周建华; 傅春燕

    2008-01-01

    目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,FGF-2)对小细胞肺癌(small cell lung cancer,SCLC)细胞株NCI-H446中survivin的表达及Smac亚细胞定位的影响.方法:Western 印迹分析FGF-2对NCI-H446中凋亡抑制蛋白survivin表达的影响.Western印迹和免疫荧光分析FGF-2对线粒体释放的促凋亡蛋白Smac的亚细胞定位的影响.流式细胞术检测NCI-H446的细胞凋亡百分率.Hoechst 33258染色观察FGF-2对NCI-H446细胞凋亡的影响.结果:FGF-2能明显诱导NCI-H446细胞中survivin蛋白的表达,survivin的表达水平与FGF-2作用的浓度和时间明显相关.血清饥饿诱导的Smac蛋白从线粒体的释放可被FGF-2所抑制.FGF-2能明显抑制血清饥饿诱导的凋亡.结论:FGF-2可显著提高SCLC细胞株NCI-H446中凋亡抑制蛋白survivin的表达水平,并显著抑制血清饥饿诱导的促凋亡蛋白Smac释放入胞浆.这可能与其抑制肿瘤细胞凋亡有关.

  1. Flight Record of the Long March Series of Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Ying

    2010-01-01

    @@ (Continued) THE 56TH LAUNCH The FY-1C meteorological satellite and the Shijian 5 (SJ-5) satellite were put into their predetermined orbits by a LM-4B launch vehicle on May 10,1999. Launch Site: Taiyuan Satellite Launch Center Launch Result: Success At 09:33 on May 10, a LM-4B lifted off with two satellites.749 seconds after the lift-off, the FY-1C satellite separated with the rocket, and the SJ-5 satellite separated with LM-4B 814 seconds after it was fired.The two satellites entered sun-synchronous orbit which is 870km above the Earth.

  2. Motivation for Air-Launch: Past, Present, and Future

    Science.gov (United States)

    Kelly, John W.; Rogers, Charles E.; Brierly, Gregory T.; Martin, J Campbell; Murphy, Marshall G.

    2017-01-01

    Air-launch is defined as two or more air-vehicles joined and working together, that eventually separate in flight, and that have a combined performance greater than the sum of the individual parts. The use of the air-launch concept has taken many forms across civil, commercial, and military contexts throughout the history of aviation. Air-launch techniques have been applied for entertainment, movement of materiel and personnel, efficient execution of aeronautical research, increasing aircraft range, and enabling flexible and efficient launch of space vehicles. For each air-launch application identified in the paper, the motivation for that application is discussed.

  3. 加速溶剂萃取GC-NCI-MS法测定糙米中氯硝胺残留量%Detection of Dicloran Residue in Crude Rice by the Combination of Accelerated Solvent Extraction and GC-NCI-MS

    Institute of Scientific and Technical Information of China (English)

    何理; 吴晖

    2012-01-01

    [目的]建立一种采用加速溶剂萃取和气相色谱-质谱测定糙米中氯硝胺残留量的分析方法.[方法]试样采用丙酮-正己烷(体积比1:2)萃取,中性氧化铝固相萃取小柱净化.用HP-FFAP石英毛细管柱程序升温分离,GC-NCI-MS的选择离子监测方式(SIM)测定,外标法定量.并对提取溶剂、萃取温度、不同冲洗体积、静态萃取时间和循环次数等试验条件进行优化.[结果]当添加水平为1、2、10、20、100 μg/kg时,方法回收率为93.6% ~ 102.8%,相对标准偏差为2.2%~6.2%,检出限是1μg/kg.采用保留时间和选择监视离子丰度比对阳性样品进行确证,加速溶剂萃取法和固相萃取技术净化糙米中氯硝胺减少了分析时间和溶剂消耗.[结论]试验成功建立了GC-NC-MS的选择离子监测的测定方法测定糙米中氯硝胺残留量,有效地减少基质杂质对分析灵敏度和方法检出限的影响,提高了检测方法的特异性.%[Objective] To establish a new method of accelerated solvent extraction and gas chromatography mass spectrography to determine the dicloran residue in kown rice. [ Method] The sample was extracted by hexane-acetone (1: 2 by volume) , purified by neutral alumina solid phase extraction column, separated by HP-FFAP silica capillary column with temperature programmed, assessed by GC-NCI-MS in selected ion monitoring mode (SIM) , and quantified by external standard. The experimental conditions including extraction solvent, extraction temperature , different flush volume, static extraction time and cycle number have been optimized. [ Result] When the adding level is 1, 2, 10, 20, 100 μg/kg, the method recovery is 93.6% -102.8% ; the relative standard deviation is 2.2% -6.2% and the detection limit is 1 |xg/kg. The result was determined by comparison to positive sample with retention time and selectively monitoring ion abundances. The accelerated solvent extraction and GC-NCI-MS method purified the

  4. Artist's Concept of Magnetic Launch Assisted Air-Breathing Rocket

    Science.gov (United States)

    1999-01-01

    This artist's concept depicts a Magnetic Launch Assist vehicle in orbit. Formerly referred to as the Magnetic Levitation (Maglev) system, the Magnetic Launch Assist system is a launch system developed and tested by engineers at the Marshall Space Flight Center (MSFC) that could levitate and accelerate a launch vehicle along a track at high speeds before it leaves the ground. Using electricity and magnetic fields, a Magnetic Launch Assist system would drive a spacecraft along a horizontal track until it reaches desired speeds. The system is similar to high-speed trains and roller coasters that use high-strength magnets to lift and propel a vehicle a couple of inches above a guideway. A full-scale, operational track would be about 1.5-miles long, capable of accelerating a vehicle to 600 mph in 9.5 seconds, and the vehicle would then shift to rocket engines for launch into orbit. The major advantages of launch assist for NASA launch vehicles is that it reduces the weight of the take-off, the landing gear, the wing size, and less propellant resulting in significant cost savings. The US Navy and the British MOD (Ministry of Defense) are planning to use magnetic launch assist for their next generation aircraft carriers as the aircraft launch system. The US Army is considering using this technology for launching target drones for anti-aircraft training.

  5. Launching facility constraints on the Space Exploration Initiative

    Science.gov (United States)

    Chan, Kadett; Montoya, Alex J.

    A quantitative tool is developed for envisioning, evaluating, and optimizing the ground and launch operations in order to meet Space Exploration Initiative (SEI) objectives. These objectives include the establishment and operation of the Space Station Freedom, lunar missions, and Mars missions. A Simulation of Logistics model (SIMLOG) is developed to assess which facilities and operations limit the maximum launch rate. This model produces the maximum achievable launch rate for each individual vehicle. The maximum launch rates are then input data for the Launch Vehicle Selection Model (LVSM), a linear integer programming model which selects the optimal number of each launch vehicle from a number of existing and proposed vehicles in order to minimize the overall multiyear launching cost of the SEI program. The simulation indicates that the SEI LEO requirement of 2.1 million lbs can be met with a mixed fleet consisting of current vehicles, a Shuttle C, and the proposed HLLV. Other results are also reported.

  6. Business Intelligence Modeling in Launch Operations

    Science.gov (United States)

    Bardina, Jorge E.; Thirumalainambi, Rajkumar; Davis, Rodney D.

    2005-01-01

    This technology project is to advance an integrated Planning and Management Simulation Model for evaluation of risks, costs, and reliability of launch systems from Earth to Orbit for Space Exploration. The approach builds on research done in the NASA ARC/KSC developed Virtual Test Bed (VTB) to integrate architectural, operations process, and mission simulations for the purpose of evaluating enterprise level strategies to reduce cost, improve systems operability, and reduce mission risks. The objectives are to understand the interdependency of architecture and process on recurring launch cost of operations, provide management a tool for assessing systems safety and dependability versus cost, and leverage lessons learned and empirical models from Shuttle and International Space Station to validate models applied to Exploration. The systems-of-systems concept is built to balance the conflicting objectives of safety, reliability, and process strategy in order to achieve long term sustainability. A planning and analysis test bed is needed for evaluation of enterprise level options and strategies for transit and launch systems as well as surface and orbital systems. This environment can also support agency simulation .based acquisition process objectives. The technology development approach is based on the collaborative effort set forth in the VTB's integrating operations. process models, systems and environment models, and cost models as a comprehensive disciplined enterprise analysis environment. Significant emphasis is being placed on adapting root cause from existing Shuttle operations to exploration. Technical challenges include cost model validation, integration of parametric models with discrete event process and systems simulations. and large-scale simulation integration. The enterprise architecture is required for coherent integration of systems models. It will also require a plan for evolution over the life of the program. The proposed technology will produce

  7. NASA's Space Launch System Program Update

    Science.gov (United States)

    May, Todd; Lyles, Garry

    2015-01-01

    Hardware and software for the world's most powerful launch vehicle for exploration is being welded, assembled, and tested today in high bays, clean rooms and test stands across the United States. NASA's Space Launch System (SLS) continued to make significant progress in 2014 with more planned for 2015, including firing tests of both main propulsion elements and the program Critical Design Review (CDR). Developed with the goals of safety, affordability, and sustainability, SLS will still deliver unmatched capability for human and robotic exploration. The initial Block 1 configuration will deliver more than 70 metric tons of payload to low Earth orbit (LEO). The evolved Block 2 design will deliver some 130 metric tons to LEO. Both designs offer enormous opportunity and flexibility for larger payloads, simplifying payload design as well as ground and on-orbit operations, shortening interplanetary transit times, and decreasing overall mission risk. Over the past year, every vehicle element has manufactured or tested hardware. An RS-25 liquid propellant engine was hotfire-tested at NASA's Stennis Space Center, Miss. for the first time since 2009 exercising and validating the new engine controller, the renovated A-1 test stand, and the test teams. Four RS-25s will power the SLS core stage. A qualification five-segment solid rocket motor incorporating several design, material, and process changes was scheduled to be test-fired in March at the prime contractor's facility in Utah. The booster also successfully completed its Critical Design Review (CDR) validating the planned design. All six major manufacturing tools for the core stage are in place at the Michoud Assembly Facility in Louisiana, and have been used to build numerous pieces of confidence, qualification, and even flight hardware, including barrel sections, domes and rings used to assemble the world's largest rocket stage. SLS Systems Engineering accomplished several key tasks including vehicle avionics software

  8. Mars Exploration Rovers Launch Contingency Efforts

    Science.gov (United States)

    McGrath, Brian E.; Frostbutter, David A.; Parthasarathy, Karungulam N.; Heyler, Gene A.; Chang, Yale

    2004-02-01

    On 10 June 2003 at 1:58 p.m. Eastern Daylight Time (EDT) and 7 July 2003 at 11:18 p.m. EDT, two separate spacecraft/rovers were successfully launched to Mars atop a Delta II 7925 and Delta II 7925H, respectively. Each spacecraft/rover carried eight Light Weight Radioisotope Heater Units (LWRHUs) for thermal conditioning of electronics during the cold Martian nights. As a part of the joint National Aeronautics and Space Administration/U. S. Department of Energy safety effort, a contingency plan was prepared to address the unlikely events of an accidental suborbital reentry or out-of-orbit reentry. The objective of the contingency plan was to develop and implement procedures to predict, within the first hour, the probable Earth Impact Footprints (EIFs) for the LWRHUs or other possible spacecraft debris after an accidental reentry. No ablation burn-through of the heat sources' aeroshells was expected, as a result of earlier testing. Any predictions would be used in subsequent notification and recovery efforts. The Johns Hopkins University Applied Physics Laboratory, as part of a multi-agency team, was responsible for prediction of the EIFs, and the time of reentry from a potential orbital decay. The tools used to predict the EIFs included a Three-Degree-of-Freedom (3DOF) trajectory simulation code, a Six-Degree-of-Freedom (6DOF) code, a database of aerodynamic coefficients for the LWRHUs and other spacecraft debris, secure links to obtain tracking data, and a high fidelity special perturbation orbit integrator code to predict time of spacecraft reentry from orbital decay. This paper will discuss the contingency plan and process, as well as highlight the improvements made to the analytical tools. Improvements to the 3DOF, aerodynamic database, and orbit integrator and inclusion of the 6DOF have significantly enhanced the prediction capabilities. In the days before launch, the trajectory simulation codes were exercised and predictions of hypothetical EIFs were produced

  9. Business intelligence modeling in launch operations

    Science.gov (United States)

    Bardina, Jorge E.; Thirumalainambi, Rajkumar; Davis, Rodney D.

    2005-05-01

    The future of business intelligence in space exploration will focus on the intelligent system-of-systems real-time enterprise. In present business intelligence, a number of technologies that are most relevant to space exploration are experiencing the greatest change. Emerging patterns of set of processes rather than organizational units leading to end-to-end automation is becoming a major objective of enterprise information technology. The cost element is a leading factor of future exploration systems. This technology project is to advance an integrated Planning and Management Simulation Model for evaluation of risks, costs, and reliability of launch systems from Earth to Orbit for Space Exploration. The approach builds on research done in the NASA ARC/KSC developed Virtual Test Bed (VTB) to integrate architectural, operations process, and mission simulations for the purpose of evaluating enterprise level strategies to reduce cost, improve systems operability, and reduce mission risks. The objectives are to understand the interdependency of architecture and process on recurring launch cost of operations, provide management a tool for assessing systems safety and dependability versus cost, and leverage lessons learned and empirical models from Shuttle and International Space Station to validate models applied to Exploration. The systems-of-systems concept is built to balance the conflicting objectives of safety, reliability, and process strategy in order to achieve long term sustainability. A planning and analysis test bed is needed for evaluation of enterprise level options and strategies for transit and launch systems as well as surface and orbital systems. This environment can also support agency simulation based acquisition process objectives. The technology development approach is based on the collaborative effort set forth in the VTB's integrating operations, process models, systems and environment models, and cost models as a comprehensive disciplined

  10. Non-small-cell lung cancer cell lines A549 and NCI-H460 express hypoxanthine guanine phosphoribosyltransferase on the plasma membrane.

    Science.gov (United States)

    Townsend, Michelle H; Anderson, Michael D; Weagel, Evita G; Velazquez, Edwin J; Weber, K Scott; Robison, Richard A; O'Neill, Kim L

    2017-01-01

    In both males and females, lung cancer is one of the most lethal cancers worldwide and accounts for >30% of cancer-related deaths. Despite advances in biomarker analysis and tumor characterization, there remains a need to find suitable biomarker antigen targets for treatment in late-stage lung cancer. Previous research on the salvage pathway enzyme TK1 shows a unique relationship with cancer patients as serum levels are raised according to cancer grade. To expand this analysis, the other salvage pathway enzymes were evaluated for possible upregulation within lung cancer. Adenine phosphoribosyltransferase, deoxycytidine kinase, and hypoxanthine guanine phosphoribosyltransferase (HPRT) were assessed for their presentation on two non-small-cell lung cancer cell lines NCI-H460 and A549. In the present study, we show that deoxycytidine kinase and adenine phosphoribosyltransferase have no significant relationship with the membrane of NCI-H460 cells. However, we found significant localization of HPRT to the membrane of NCI-H460 and A549 cells. When treated with anti-HPRT antibodies, the average fluorescence of the cell population increased by 24.3% and 12.9% in NCI-H460 and A549 cells, respectively, in comparison with controls. To ensure that expression was not attributed to cytoplasmic HPRT, confocal microscopy was performed to visualize HPRT binding on the plasma membrane. After staining NCI-H460 cells treated with both fluorescent antibodies and a membrane-specific dye, we observed direct overlap between HPRT and the membrane of the cancer cells. Additionally, gold-conjugated antibodies were used to label and quantify the amount of HPRT on the cell surface using scanning electron microscopy and energy-dispersive analysis X-ray. Further confirming HPRT presence, the gold weight percentage of the sample increased significantly when NCI-H460 cells were exposed to HPRT antibody (P=0.012) in comparison with isotype controls. Our results show that HPRT is localized on the

  11. Non-small-cell lung cancer cell lines A549 and NCI-H460 express hypoxanthine guanine phosphoribosyltransferase on the plasma membrane

    Science.gov (United States)

    Townsend, Michelle H; Anderson, Michael D; Weagel, Evita G; Velazquez, Edwin J; Weber, K Scott; Robison, Richard A; O’Neill, Kim L

    2017-01-01

    In both males and females, lung cancer is one of the most lethal cancers worldwide and accounts for >30% of cancer-related deaths. Despite advances in biomarker analysis and tumor characterization, there remains a need to find suitable biomarker antigen targets for treatment in late-stage lung cancer. Previous research on the salvage pathway enzyme TK1 shows a unique relationship with cancer patients as serum levels are raised according to cancer grade. To expand this analysis, the other salvage pathway enzymes were evaluated for possible upregulation within lung cancer. Adenine phosphoribosyltransferase, deoxycytidine kinase, and hypoxanthine guanine phosphoribosyltransferase (HPRT) were assessed for their presentation on two non-small-cell lung cancer cell lines NCI-H460 and A549. In the present study, we show that deoxycytidine kinase and adenine phosphoribosyltransferase have no significant relationship with the membrane of NCI-H460 cells. However, we found significant localization of HPRT to the membrane of NCI-H460 and A549 cells. When treated with anti-HPRT antibodies, the average fluorescence of the cell population increased by 24.3% and 12.9% in NCI-H460 and A549 cells, respectively, in comparison with controls. To ensure that expression was not attributed to cytoplasmic HPRT, confocal microscopy was performed to visualize HPRT binding on the plasma membrane. After staining NCI-H460 cells treated with both fluorescent antibodies and a membrane-specific dye, we observed direct overlap between HPRT and the membrane of the cancer cells. Additionally, gold-conjugated antibodies were used to label and quantify the amount of HPRT on the cell surface using scanning electron microscopy and energy-dispersive analysis X-ray. Further confirming HPRT presence, the gold weight percentage of the sample increased significantly when NCI-H460 cells were exposed to HPRT antibody (P=0.012) in comparison with isotype controls. Our results show that HPRT is localized on the

  12. Launch of technical training courses for programmers

    CERN Multimedia

    2015-01-01

    This autumn, two new technical training courses have been launched for scientists and engineers at CERN who undertake programming tasks, particularly in C and C++. Both courses are taught by Andrzej Nowak, an expert in next-generation and cutting-edge computing technology research.   The training courses are organised in cooperation with CERN openlab and are sponsored by the CERN IT department – there is only a nominal registration fee of 50 CHF. This is an opportunity not to be missed! Computer architecture and hardware-software interaction (2 days, 26-27 October) The architecture course offers a comprehensive overview of current topics in computer architecture and their consequences for the programmer, from the basic Von Neumann schema to its modern-day expansions. Understanding hardware-software interaction allows the programmer to make better use of all features of available computer hardware and compilers. Specific architectural ...

  13. Integrated Launch Operations Applications Remote Display Developer

    Science.gov (United States)

    Flemming, Cedric M., II

    2014-01-01

    This internship provides the opportunity to support the creation and use of Firing Room Displays and Firing Room Applications that use an abstraction layer called the Application Control Language (ACL). Required training included video watching, reading assignments, face-to-face instruction and job shadowing other Firing Room software developers as they completed their daily duties. During the training period various computer and access rights needed for creating the applications were obtained. The specific ground subsystems supported are the Cryogenics Subsystems, Liquid Hydrogen (LH2) and Liquid Oxygen (LO2). The cryogenics team is given the task of finding the best way to handle these very volatile liquids that are used to fuel the Space Launch System (SLS) and the Orion flight vehicles safely.

  14. Aerodynamic Characterization of a Modern Launch Vehicle

    Science.gov (United States)

    Hall, Robert M.; Holland, Scott D.; Blevins, John A.

    2011-01-01

    A modern launch vehicle is by necessity an extremely integrated design. The accurate characterization of its aerodynamic characteristics is essential to determine design loads, to design flight control laws, and to establish performance. The NASA Ares Aerodynamics Panel has been responsible for technical planning, execution, and vetting of the aerodynamic characterization of the Ares I vehicle. An aerodynamics team supporting the Panel consists of wind tunnel engineers, computational engineers, database engineers, and other analysts that address topics such as uncertainty quantification. The team resides at three NASA centers: Langley Research Center, Marshall Space Flight Center, and Ames Research Center. The Panel has developed strategies to synergistically combine both the wind tunnel efforts and the computational efforts with the goal of validating the computations. Selected examples highlight key flow physics and, where possible, the fidelity of the comparisons between wind tunnel results and the computations. Lessons learned summarize what has been gleaned during the project and can be useful for other vehicle development projects.

  15. The CERN & Society programme launches its newsletter

    CERN Multimedia

    Matteo Castoldi

    2016-01-01

    The newsletter will be issued quarterly. Sign up to remain informed about the latest initiatives of the CERN & Society programme!    The CERN & Society programme encompasses projects in the areas of education and outreach, innovation and knowledge exchange, and culture and creativity that spread the CERN spirit of scientific curiosity for the inspiration and benefit of society. The programme is funded primarily by the CERN & Society Foundation, a charitable foundation established by CERN and supported by individuals, trusts, organisations and commercial companies. The projects are inspired or enabled by CERN but lie outside of the Laboratory’s specific research mandate. We especially want to help young talent from around the world to flourish in the future. The programme is now launching its newsletter, which will be issued quarterly. Everybody who wants to be informed about CERN & Society’s activities, stay up-to-date with its latest in...

  16. Europe's first Moon probe prepares for launch

    Science.gov (United States)

    2003-08-01

    The European Space Agency’s SMART-1 spacecraft was delivered to Kourou, French Guiana, on July 15 and is currently being prepared for launch atop an Ariane 5 during the night from August 28 to 29. The launch window will open at 20:04 local time (01:04 on August 29 morning CEST) and will remain open for26 minutes. The 367 kg spacecraft will share Ariane’s V162 launch with two commercial payloads: the Indian Space Research Organisation’s Insat 3E and Eutelsat’s e-Bird communication satellites. The smallest spacecraft in the trio, SMART-1, will travel in the lower position, inside a cylindrical adapter, and will be the last to be released. A generic Ariane 5 will be in charge of placing these three payloads in a standard geostationary transfer orbit from which each will begin its own journey towards its final operational orbit. SMART-1, powered by its ion engine, will reach its destination in about 16 months, having followed a long spiralling trajectory. SMART-1’s ion engine will be used to accelerate the probe and raise its orbit until it reaches the vicinity of the Moon, some 350,000 to 400,000 km from Earth. Then, following gravity assists from a series of lunar swingbys in late September, late October and late November 2004, SMART-1 will be “captured” by the Moon’s gravity in December 2004 and will begin using its engine to slow down and reduce the altitude of its lunar orbit. Testing breakthrough technologies and studying the Moon SMART-1 is not a standard outer space probe. As ESA’s first Small Mission for Advanced Research in Technology, it is primarily designed to demonstrate innovative and key technologies for future deep space science missions. However, once it has arrived at its destination, it will also perform an unprecedented scientific study of the Moon. SMART-1 is a very small spacecraft (measuring just one cubic metre). Its solar arrays, spanning 14 metres, will deliver 1.9 kW of power, about 75% of which will be used for the probe

  17. Experiències de realitat augmentada en biblioteques : estat de la qüestió

    Directory of Open Access Journals (Sweden)

    Arroyo Vázquez, Natalia

    2016-06-01

    Full Text Available Objectiu: donar a conèixer les experiències més significatives d'ús de la realitat augmentada en biblioteques, amb una especial atenció als resultats obtinguts, les aportacions i les limitacions que s'han de tenir en compte. -- Metodologia: revisió bibliogràfica, selecció i anàlisi d'experiències sobre l'ús de realitat augmentada en biblioteques. -- Resultats: tot i ser una tecnologia recent, són diversos els exemples d'ús de la realitat augmentada en biblioteques. No obstant això, es fa necessari donar a conèixer els resultats d'aquestes experiències, de manera que puguin servir no solament com a model, sinó també per conèixer què és el que funciona. Es presenta als professionals un catàleg d'usos de la realitat augmentada en biblioteques, dels quals s'analitzen de forma crítica els possibles beneficis i limitacions, i s'agrupen en set apartats segons la utilitat: geolocalització, contextualització històrica, exposicions i altres activitats, publicacions, enriquiment dels espais físics, alfabetització i ludificació i, finalment, usos professionals.Objetivo: dar a conocer las experiencias más significativas de uso de la realidad aumentada en bibliotecas, con una especial atención a los resultados obtenidos, las aportaciones y las limitaciones que se deben tener en cuenta. -- Metodología: revisión bibliográfica, selección y análisis de experiencias sobre el uso de realidad aumentada en bibliotecas. -- Resultados: a pesar de ser una tecnología reciente, son varios los ejemplos de uso de la realidad aumentada en bibliotecas. Sin embargo, se hace necesario dar a conocer los resultados de dichas experiencias, de forma que puedan servir no solo como modelo, sino también para conocer qué es lo que funciona. Se presenta a los profesionales un catálogo de usos de la realidad aumentada en bibliotecas, analizados de forma crítica sus posibles beneficios y limitaciones, agrupados en siete apartados según la utilidad

  18. Experiències de realitat augmentada en biblioteques : estat de la qüestió

    Directory of Open Access Journals (Sweden)

    Arroyo Vázquez, Natalia

    2016-06-01

    Full Text Available Objectiu: donar a conèixer les experiències més significatives d'ús de la realitat augmentada en biblioteques, fent una especial atenció als resultats obtinguts, les aportacions i les limitacions que s'han de tenir en compte. -- Metodologia: revisió bibliogràfica, selecció i anàlisi d'experiències sobre l'ús de la realitat augmentada en biblioteques. -- Resultats: malgrat ser una tecnologia recent, els exemples d'ús de la realitat augmentada en biblioteques són diversos. No obstant això, es fa necessari donar a conèixer els resultats d'aquestes experiències, de manera que puguin servir no solament com a model, sinó també per conèixer què és el que funciona. Es presenta als professionals un catàleg d'usos de la realitat augmentada en biblioteques, dels quals s'analitzen de forma crítica els possibles beneficis i limitacions, i s'agrupen en set apartats segons la utilitat: geolocalització, contextualització històrica, exposicions i altres activitats, publicacions, enriquiment dels espais físics, alfabetització i ludificació i, finalment, usos professionals.Objetivo: dar a conocer las experiencias más significativas de uso de la realidad aumentada en bibliotecas, con una especial atención a los resultados obtenidos, las aportaciones y las limitaciones que se deben tener en cuenta. -- Metodología: revisión bibliográfica, selección y análisis de experiencias sobre el uso de la realidad aumentada en bibliotecas. -- Resultados: a pesar de ser una tecnología reciente, son varios los ejemplos de uso de la realidad aumentada en bibliotecas. Sin embargo, se hace necesario dar a conocer los resultados de dichas experiencias, de forma que puedan servir no solo como modelo, sino también para conocer qué es lo que funciona. Se presenta a los profesionales un catálogo de usos de la realidad aumentada en bibliotecas, analizados de forma crítica sus posibles beneficios y limitaciones, agrupados en siete apartados según la

  19. Rockot Launch Vehicle Commercial Operations for Grace and Iridium Program

    Science.gov (United States)

    Viertel, Y.; Kinnersley, M.; Schumacher, I.

    2002-01-01

    The GRACE mission and the IRIDIUM mission on ROCKOT launch vehicle are presented. Two identical GRACE satellites to measure in tandem the gravitational field of the earth with previously unattainable accuracy - it's called the Gravity Research and Climate Experiment, or and is a joint project of the U.S. space agency, NASA and the German Centre for Aeronautics and Space Flight, DLR. In order to send the GRACE twins into a 500x500 km , 89deg. orbit, the Rockot launch vehicle was selected. A dual launch of two Iridium satellites was scheduled for June 2002 using the ROCKOT launch vehicle from Plesetsk Cosmodrome in Northern Russia. This launch will inject two replacement satellites into a low earth orbit (LEO) to support the maintenance of the Iridium constellation. In September 2001, Eurockot successfully carried out a "Pathfinder Campaign" to simulate the entire Iridium mission cycle at Plesetsk. The campaign comprised the transport of simulators and related equipment to the Russian port-of-entry and launch site and also included the integration and encapsulation of the simulators with the actual Rockot launch vehicle at Eurockot's dedicated launch facilities at Plesetsk Cosmodrome. The pathfinder campaign lasted four weeks and was carried out by a joint team that also included Khrunichev, Russian Space Forces and Eurockot personnel on the contractors' side. The pathfinder mission confirmed the capability of Eurockot Launch Services to perform the Iridium launch on cost and on schedule at Plesetsk following Eurockot's major investment in international standard preparation, integration and launch facilities including customer facilities and a new hotel. In 2003, Eurockot will also launch the Japanese SERVI'S-1 satellite for USEF. The ROCKOT launch vehicle is a 3 stage liquid fuel rocket whose first 2 stages have been adapted from the Russian SS-19. A third stage, called "Breeze", can be repeatedly ignited and is extraordinarily capable of manoeuvre. Rockot can place

  20. Space Launch System Spacecraft and Payload Elements: Making Progress Toward First Launch

    Science.gov (United States)

    Schorr, Andrew A.; Creech, Stephen D.

    2016-01-01

    Significant and substantial progress continues to be accomplished in the design, development, and testing of the Space Launch System (SLS), the most powerful human-rated launch vehicle the United States has ever undertaken. Designed to support human missions into deep space, SLS is one of three programs being managed by the National Aeronautics and Space Administration's (NASA's) Exploration Systems Development directorate. The Orion spacecraft program is developing a new crew vehicle that will support human missions beyond low Earth orbit, and the Ground Systems Development and Operations program is transforming Kennedy Space Center into next-generation spaceport capable of supporting not only SLS but also multiple commercial users. Together, these systems will support human exploration missions into the proving ground of cislunar space and ultimately to Mars. SLS will deliver a near-term heavy-lift capability for the nation with its 70 metric ton (t) Block 1 configuration, and will then evolve to an ultimate capability of 130 t. The SLS program marked a major milestone with the successful completion of the Critical Design Review in which detailed designs were reviewed and subsequently approved for proceeding with full-scale production. This marks the first time an exploration class vehicle has passed that major milestone since the Saturn V vehicle launched astronauts in the 1960s during the Apollo program. Each element of the vehicle now has flight hardware in production in support of the initial flight of the SLS -- Exploration Mission-1 (EM-1), an un-crewed mission to orbit the moon and return. Encompassing hardware qualification, structural testing to validate hardware compliance and analytical modeling, progress in on track to meet the initial targeted launch date in 2018. In Utah and Mississippi, booster and engine testing are verifying upgrades made to proven shuttle hardware. At Michoud Assembly Facility in Louisiana, the world's largest spacecraft welding

  1. Incorporació de petites seqüències de cinema comercial en l’ensenyament de les drogodependències. Assaig pilot en l'assignatura de Toxicologia

    Directory of Open Access Journals (Sweden)

    Miguel Rodamilans-Pérez

    2013-01-01

    Full Text Available El Grup d'Innovació Docent Orfila, en el seu projecte per millorar la qualitat de la docència, està assajant la utilització del cinema amb finalitat didàctica. El material didàctic que hem desenvolupat en aquest projecte són petites seqüències de pel·lícules comercials de 3 a 5 minuts, per ser utilitzades com a elements il·lustratius del procés addictiu. Se seleccionen escenes de la filmografia i s'adeqüen als nostres programes docents. Es recull l'opinió dels professors participants, així com la dels alumnes, mitjançant una entrevista personal i una enquesta d'opinió, respectivament.De les entrevistes als professors i de les enquestes d'opinió dels alumnes, es dedueix un alt grau de satisfacció.

  2. El concepte de futur en l’ensenyament de les ciències socials. Estudis de cas a l'educació secundària

    OpenAIRE

    Anguera Cerarols, Carles

    2012-01-01

    Tota educació és una preparació per al futur. Actualment, en les ciències socials, el passat i el present són les categories temporals que més atenció reben, si no són les úniques. En aquesta situació, la construcció de les representacions socials del futur i el seu treball, queden en mans dels mitjans de comunicació i altres experiències fora del marc educatiu. Provocant així un buit en la formació de l'alumnat. L'educació per al futur pertany als denominats future studies o més coneguts a E...

  3. Competències desenvolupades en una experiència internacional i empleabilitat: una perspectiva de gènere

    Directory of Open Access Journals (Sweden)

    Javier Barraycoa

    2012-06-01

    Full Text Available El objectiu principal d’aquest estudi es esbrinar si l’experiència internacional genera diferents expectatives i discursos en funció del gènere del participants pel que fa al desenvolupament de competències i utilitat per a l’ocupabilitat. Per això s’analitzen els resultats d’un qüestionari que ha estat respost per una vintena d’estudiants i recent llicenciats de La universitat Abat Oliba. Las diferències entre homes i dones tant en el desenvolupament de l’estada internacional com en la seva valoració pel que fa a la futura ocupabilitat faciliten elements interessants per a la creació de protocols que permetin optimitzar aquest tipus d’estades.

  4. Association of a single nucleotide polymorphic variation in the human chromosome 19q13.3 with drug responses in the NCI60 cell lines

    DEFF Research Database (Denmark)

    Nissen, K.K.; Vogel, Ulla Birgitte; Nexo, B.A.

    2009-01-01

    We studied the importance of certain polymorphisms on human chromosome 19q13.3 for drug sensitivity in human tumor cell cultures. NCI60 is a panel of 60 established tumor-derived cell lines, which have been tested for their sensitivity to tens of thousands of different drugs. Here we investigate...... the correlations between the responses of the NCI60 cells to different anticancer drugs and their respective alleles of five DNA polymorphisms located in a cancer-related chromosomal area. One polymorphism, located in the 5' noncoding region of the gene ASE-1, alias CD3EAP, proved to be associated with drug...... sensitivity (P=0.025). The same polymorphism has previously been associated with treatment response of multiple myeloma after bone marrow ablation. The polymorphism ASE-1-e1 was of importance for the drug response in the human cancer cell lines investigated and could eventually become important...

  5. L’avaluació de competències a l’Educació Superior: el cas d’un màster universitari

    Directory of Open Access Journals (Sweden)

    Xavier Ma. Triadó i Ivern

    2013-01-01

    Full Text Available La implantació de les competències és una tasca que ha anat incorporant-se paulatinament pels docents de la universitat espanyola amb l’entrada en vigor del EEES. Tot i això, encara s’està lluny d’aconseguir nivells òptims d’avaluació de les mateixes. Aquest article permet reflexionar sobre algunes bones practiques al respecte i sobre les dificultats i limitacions que apareixen en voler implementar un canvi en les metodologies docents, en el marc d’un màster universitari. Els resultats indiquen el grau en què s’han avaluat i adquirit tant les competències genèriques com especifiques en l’educació superior.

  6. Peptide production and secretion in GLUTag, NCI-H716 and STC-1 cells: a comparison to native L-cells

    DEFF Research Database (Denmark)

    Kuhre, Rune Ehrenreich; Albrechtsen, Nicolai Jacob Wewer; Deacon, Carolyn F.;

    2016-01-01

    normally co-localizes with GLP-1 in distal L-cells, was not detected in any of the cell lines. GLUTag and STC-1 cells also expressed vasoactive intestinal peptide, but none expressed pancreatic polypeptide or insulin. GLUTag contained and secreted large amounts of cholecystokinin while NCI-H716 did......GLUTag, NCI-H716 and STC-1 are cell lines that are widely used to study mechanisms underlying secretion of glucagon-like peptide (GLP-1), but the extent to which they resemble native L-cells is unknown. We used validated immunoassays for 14 different hormones to analyze peptide content (lysis...... samples; n=9 from different passage numbers) or peptide secretion in response to buffer (baseline), and after stimulation with 50 mM KCl or 10 mM glucose + 10 µM forskolin/3-isobutyl-1-methylxanthine (n=6 also different passage numbers). All cell lines produced and processed proglucagon into GLP-1, GLP-2...

  7. P30 Cancer Center Support Grant Administrative Supplements to NCI-designated Cancer Centers not affiliated with the Experimental Therapeutics Clinical Trials Network (ETCTN) to support participation in the ETCTN

    Science.gov (United States)

    P30 Cancer Center Support Grant Administrative Supplements to NCI-designated Cancer Centers not affiliated with the Experimental Therapeutics Clinical Trials Network (ETCTN) to support participation in the ETCTN

  8. Europe looks forward to COROT launch

    Science.gov (United States)

    2006-12-01

    While CNES is completing preparations for the launch from Baikonur/Kazakhstan, ESA and a large number of European scientists involved in the mission are eagerly awaiting this event and the first scientific results to come through. What is COROT? COROT stands for ‘Convection Rotation and planetary Transits’. The name describes the mission’s scientific goals. ‘Convection and rotation’ refer to the satellite’s capability to probe stellar interiors, studying the acoustic waves that ripple across the surface of stars, a technique called asteroseismology. ‘Transit’ refers to the technique whereby the presence of a planet orbiting a star can be inferred from the dimming starlight caused when the planet passes in front of it. To achieve its twin scientific objectives, COROT will monitor some 120,000 stars with its 30-centimetre telescope. COROT will lead a bold new search for planets around other stars. In the decade since the first discovery in 1995 of an exoplanet (51 Pegasi b), more than 200 other such planets outside our solar system have been detected using ground-based observatories. The COROT space telescope promises to find many more during its two-and-a-half-year mission, expanding the frontiers of our knowledge towards ever-smaller planets. Many of the planets COROT will detect are expected to be 'hot Jupiters', gaseous worlds. An unknown percentage of those detected are expected to be rocky planets, maybe just a few times larger than the Earth (or smaller, even). If COROT finds such planets, they will constitute a new class of planet altogether. While it is looking at a star, COROT will also be able to detect 'starquakes', acoustic waves generated deep inside a star that send ripples across its surface, altering its brightness. The exact nature of the ripples allows astronomers to calculate the star's precise mass, age and chemical composition. COROT’s European dimension The COROT mission was first proposed by CNES back in 1996. A call for

  9. Underusers of mammogram screening: stage of adoption in five U.S. subpopulations. The NCI Breast Cancer Screening Consortium.

    Science.gov (United States)

    Stoddard, A M; Rimer, B K; Lane, D; Fox, S A; Lipkus, I; Luckmann, R; Avrunin, J S; Sprachman, S; Costanza, M; Urban, N

    1998-01-01

    The purpose of this report is to describe the characteristics of women ages 50 to 80 who do not follow commonly accepted mammography screening guidelines. It provides unique understanding of the robustness of characteristics of underusers across five different U.S. subpopulations. The data are from the baseline surveys of the five studies of the NCI Breast Cancer Screening Consortium. Stage of adoption of mammography screening and other characteristics of underusers are presented. Polytomous logistic regression analysis was used to explore multivariable associations with stage of adoption in each study site. The five samples studied by the Consortium range in size from 259 to 4,477 women (n = 11,292). The relationship of the perceptions of the pros and cons of mammography with stage of adoption was strikingly similar across the five samples. Other variables consistently associated with stage were a recent receipt of a breast physical examination and recommendation for mammography by a physician. The findings suggest a need to encourage regular screening through effective communication from a health care provider. Intervention messages should be designed to increase the pros of mammography, decrease the cons, and highlight these differentially according to the woman's stage of adoption.

  10. Rapid interaction of Helicobacter pylori with microvilli of the polar human gastric epithelial cell line NCI-N87.

    Science.gov (United States)

    Diesing, Anne-Kathrin; Nossol, Constanze; Faber-Zuschratter, Heidi; Zuschratter, Werner; Renner, Lydia; Sokolova, Olga; Naumann, Michael; Rothkötter, Hermann-Josef

    2013-12-01

    Infection with Helicobacter pylori results often in chronic gastritis, gastric ulcers or even gastric tumor development. Little is known about the initial interaction between gastric epithelial cells and H. pylori. The aim of the present study was to analyze the initial host contact to the bacteria. Monolayers of the human gastric epithelial cell line NCI-N87 grown on porous membranes were used and the apical side of the epithelium was exposed to the H. pylori wild-type strain P1 for 1 hr. Many epithelial cells were colonized by bacteria within the period of 60 min. Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli. Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria. The present study demonstrates the importance of microvilli on apical epithelial cells during the initial contact of the host by colonizing H. pylori.

  11. Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis

    Directory of Open Access Journals (Sweden)

    Ooi Kheng Leong

    2011-01-01

    Full Text Available Physalis minima L. is reputed for having anticancer property. In this study, the chloroform extract of this plant exhibited remarkable cytotoxic activities on NCI-H23 (human lung adenocarcinoma cell line at dose- and time-dependent manners (after 24, 48 and 72 h of incubation. Analysis of cell-death mechanism demonstrated that the extract exerted apoptotic programed cell death in NCI-H23 cells with typical DNA fragmentation, which is a biochemical hallmark of apoptosis. Morphological observation using transmission electron microscope (TEM also displayed apoptotic characteristics in the treated cells, including clumping and margination of chromatins, followed by convolution of the nuclear and budding of the cells to produce membrane-bound apoptotic bodies. Different stages of apoptotic programed cell death as well as phosphatidylserine externalization were confirmed using annexin V and propidium iodide staining. Furthermore, acute exposure to the extract produced a significant regulation of c-myc, caspase-3 and p53 mRNA expression in this cell line. Due to its apoptotic effect on NCI-H23 cells, it is strongly suggested that the extract could be further developed as an anticancer drug.

  12. Decursin in Angelica gigas Nakai (AGN) Enhances Doxorubicin Chemosensitivity in NCI/ADR-RES Ovarian Cancer Cells via Inhibition of P-glycoprotein Expression.

    Science.gov (United States)

    Choi, Hyeong Sim; Cho, Sung-Gook; Kim, Min Kyoung; Kim, Min Soo; Moon, Seung Hee; Kim, Il Hwan; Ko, Seong-Gyu

    2016-12-01

    Angelica gigas Nakai (AGN, Korean Dang-gui) is traditionally used for the treatment of various diseases including cancer. Here, we investigated multidrug-resistant phenotype-reversal activities of AGN and its compounds (decursin, ferulic acid, and nodakenin) in doxorubicin-resistant NCI/ADR-RES ovarian cancer cells. Our results showed that a combination of doxorubicin with either AGN or decursin inhibited a proliferation of NCI/ADR-RES cells. These combinations increased the number of cells at sub-G1 phase when cells were stained with Annexin V-fluorescein isothiocyanate. We also found that these combinations activated caspase-9, caspase-8, and caspase-3 and increased cleaved PARP level. Moreover, an inhibition of P-glycoprotein expression by either AGN or decursin resulted in a reduction of its activity in NCI/ADR-RES cells. Therefore, our data demonstrate that decursin in AGN inhibits doxorubicin-resistant ovarian cancer cell proliferation and induces apoptosis in the presence of doxorubicin via blocking P-glycoprotein expression. Therefore, AGN would be a potentially novel treatment option for multidrug-resistant tumors by sensitizing to anticancer agents. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  13. Molecular Characterization of TMPRSS2-ERG Gene Fusion in the NCI-H660 Prostate Cancer Cell Line: A New Perspective for an Old Model

    Directory of Open Access Journals (Sweden)

    Kirsten D. Mertz

    2007-03-01

    Full Text Available Recent studies have established that a significant fraction of prostate cancers harbor a signature gene fusion between the 5' region of androgen-regulated TMPRSS2 and an ETS family transcription factor, most commonly ERG. Studies on the molecular mechanisms and functional consequences of this important chromosomal rearrangement are currently limited to the VCaP cell line derived from a vertebral bone metastasis of a hormone-refractory prostate tumor. Here we report on the NCI-H660 cell line, derived from a metastasic site of an extrapulmonary small cell carcinoma arising from the prostate. NCI-H660 harbors TMPRSS2-ERG fusion with a homozygous intronic deletion between TMPRSS2 and ERG. We demonstrate this by real-time quantitative polymerase chain reaction, a two-stage dual-color interphase fluorescence in situ hybridization (FISH assay testing for TMPRSS2 and ERG break-aparts, and single-nucleotide polymorphism oligonucleotide arrays. The deletion is consistent with the common intronic deletion found on chromosome 21q22.2-3 in human prostate cancer samples. We demonstrate the physical juxtaposition of TMPRSS2 and ERG on the DNA level by fiber FISH. The androgen receptor-negative NCI-H660 cell line expresses ERG in an androgen-independent fashion. This in vitro model system has the potential to provide important pathobiologic insights into TMPRSS2-ERG fusion prostate cancer.

  14. RADEM: An Air Launched, Rocket Demonstrator for Future Advanced Launch Systems

    Science.gov (United States)

    Parkinson, R. C.; Skorodelov, V. A.; Serdijk, I. I.; Neiland, V. Ya.

    1995-10-01

    Critical features associated with future reusable launch vehicles include reduction of turn around effort, use of integral liquid hydrogen tanks, advanced structures and thermal protection, and re-usable LOx-hydrogen propulsion with low maintenance overheads. Many doubts associated with such designs could be removed by a sub-orbital demonstrator. An air launched vehicle would fulfil many of the objectives for such demonstration. British Aerospace, NPO Molnija, TsAGI and DB Antonov have made an initial study for ESA for such a demonstrator (RADEM), using earlier studies of operational launch systems with the An-225 /Hotol and MAKS proposals. The paper describes the results of this study, including the selection of two potential vehicle designs, and an approach to sub-system design and vehicle development to minimize the costs. It appears that such a vheicle, capable of flying to Mach 12 or beyond using currently available technology, could have a cost an order of magnitude less than that required for development of an operational vehicle.

  15. LM-3B/E will launch Apstar 7

    Institute of Scientific and Technical Information of China (English)

    Zong He

    2009-01-01

    @@ China Great Wall Industry Corporation (CGWlC), a subsidiary of China Aerospace Science and Technology Corporation (CASC), signed a launch services contract with Hong Kong APT Satellite Co., Ltd in Beijing on November 8. According to the contract, a Long March 3B enhanced launch vehicle (LM-3B/E) will launch a French Thales Alenia Space made APstar 7 communications satellite into space in the first half year of 2012.

  16. Space Shuttle Launch Probability Analysis: Understanding History so We Can Predict the Future

    Science.gov (United States)

    Cates, Grant R.

    2014-01-01

    The Space Shuttle was launched 135 times and nearly half of those launches required 2 or more launch attempts. The Space Shuttle launch countdown historical data of 250 launch attempts provides a wealth of data that is important to analyze for strictly historical purposes as well as for use in predicting future launch vehicle launch countdown performance. This paper provides a statistical analysis of all Space Shuttle launch attempts including the empirical probability of launch on any given attempt and the cumulative probability of launch relative to the planned launch date at the start of the initial launch countdown. This information can be used to facilitate launch probability predictions of future launch vehicles such as NASA's Space Shuttle derived SLS. Understanding the cumulative probability of launch is particularly important for missions to Mars since the launch opportunities are relatively short in duration and one must wait for 2 years before a subsequent attempt can begin.

  17. Launch Period Development for the Juno Mission to Jupiter

    Science.gov (United States)

    Kowalkowski, Theresa D.; Johannesen, Jennie R.; Lam, Try

    2008-01-01

    The Juno mission to Jupiter is targeted to launch in 2011 and would reach the giant planet about five years later. The interplanetary trajectory is planned to include two large deep space maneuvers and an Earth gravity assist a little more than two years after launch. In this paper, we describe the development of a 21-day launch period for Juno with the objective of keeping overall launch energy and delta-V low while meeting constraints imposed on Earth departure, the deep space maneuvers' timing and geometry, and Jupiter arrival.

  18. Expandable External Payload Carrier for Existing Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Numerous existing launch vehicles have excess performance that is not being optimized. By taking advantage of excess, unused, performance, additional NASA...

  19. Launch Period Development for the Juno Mission to Jupiter

    Science.gov (United States)

    Kowalkowski, Theresa D.; Johannesen, Jennie R.; Lam, Try

    2008-01-01

    The Juno mission to Jupiter is targeted to launch in 2011 and would reach the giant planet about five years later. The interplanetary trajectory is planned to include two large deep space maneuvers and an Earth gravity assist a little more than two years after launch. In this paper, we describe the development of a 21-day launch period for Juno with the objective of keeping overall launch energy and delta-V low while meeting constraints imposed on Earth departure, the deep space maneuvers' timing and geometry, and Jupiter arrival.

  20. Software for Collaborative Engineering of Launch Rockets

    Science.gov (United States)

    Stanley, Thomas Troy

    2003-01-01

    The Rocket Evaluation and Cost Integration for Propulsion and Engineering software enables collaborative computing with automated exchange of information in the design and analysis of launch rockets and other complex systems. RECIPE can interact with and incorporate a variety of programs, including legacy codes, that model aspects of a system from the perspectives of different technological disciplines (e.g., aerodynamics, structures, propulsion, trajectory, aeroheating, controls, and operations) and that are used by different engineers on different computers running different operating systems. RECIPE consists mainly of (1) ISCRM a file-transfer subprogram that makes it possible for legacy codes executed in their original operating systems on their original computers to exchange data and (2) CONES an easy-to-use filewrapper subprogram that enables the integration of legacy codes. RECIPE provides a tightly integrated conceptual framework that emphasizes connectivity among the programs used by the collaborators, linking these programs in a manner that provides some configuration control while facilitating collaborative engineering tradeoff studies, including design to cost studies. In comparison with prior collaborative-engineering schemes, one based on the use of RECIPE enables fewer engineers to do more in less time.

  1. Wireless Data Acquisition System for Launch Vehicles

    Directory of Open Access Journals (Sweden)

    Sabooj Ray

    2013-03-01

    Full Text Available Present launch vehicle integration architecture for avionics uses wired link to transfer data between various sub-systems. Depending on system criticality and complexity, MIL1553 and RS485 are the common protocols that are adopted. These buses have their inherent complexity and failure issues due to harness defects or under adverse flight environments. To mitigate this problem, a prototype wireless, data acquisition system for telemetry applications has been developed and demonstrated. The wireless system simplifies the integration, while reducing weight and costs. Commercial applications of wireless systems are widespread. Few systems have recently been developed for complex and critical environments. Efforts have been underway to make such architectures operational in promising application scenarios. This paper discusses the system concept for adapting a wireless system to the existing bus topology. The protocol involved and the internal implementation of the different modules are described. The test results are presented; some of the issues faced are discussed and the; future course of action is identified.Defence Science Journal, 2013, 63(2, pp.186-191, DOI:http://dx.doi.org/10.14429/dsj.63.4262

  2. Orion Launch Abort System Performance During Exploration Flight Test 1

    Science.gov (United States)

    McCauley, Rachel; Davidson, John; Gonzalez, Guillo

    2015-01-01

    The Orion Launch Abort System Office is taking part in flight testing to enable certification that the system is capable of delivering the astronauts aboard the Orion Crew Module to a safe environment during both nominal and abort conditions. Orion is a NASA program, Exploration Flight Test 1 is managed and led by the Orion prime contractor, Lockheed Martin, and launched on a United Launch Alliance Delta IV Heavy rocket. Although the Launch Abort System Office has tested the critical systems to the Launch Abort System jettison event on the ground, the launch environment cannot be replicated completely on Earth. During Exploration Flight Test 1, the Launch Abort System was to verify the function of the jettison motor to separate the Launch Abort System from the crew module so it can continue on with the mission. Exploration Flight Test 1 was successfully flown on December 5, 2014 from Cape Canaveral Air Force Station's Space Launch Complex 37. This was the first flight test of the Launch Abort System preforming Orion nominal flight mission critical objectives. The abort motor and attitude control motors were inert for Exploration Flight Test 1, since the mission did not require abort capabilities. Exploration Flight Test 1 provides critical data that enable engineering to improve Orion's design and reduce risk for the astronauts it will protect as NASA continues to move forward on its human journey to Mars. The Exploration Flight Test 1 separation event occurred at six minutes and twenty seconds after liftoff. The separation of the Launch Abort System jettison occurs once Orion is safely through the most dynamic portion of the launch. This paper will present a brief overview of the objectives of the Launch Abort System during a nominal Orion flight. Secondly, the paper will present the performance of the Launch Abort System at it fulfilled those objectives. The lessons learned from Exploration Flight Test 1 and the other Flight Test Vehicles will certainly

  3. Space Launch System Accelerated Booster Development Cycle

    Science.gov (United States)

    Arockiam, Nicole; Whittecar, William; Edwards, Stephen

    2012-01-01

    With the retirement of the Space Shuttle, NASA is seeking to reinvigorate the national space program and recapture the public s interest in human space exploration by developing missions to the Moon, near-earth asteroids, Lagrange points, Mars, and beyond. The would-be successor to the Space Shuttle, NASA s Constellation Program, planned to take humans back to the Moon by 2020, but due to budgetary constraints was cancelled in 2010 in search of a more "affordable, sustainable, and realistic" concept2. Following a number of studies, the much anticipated Space Launch System (SLS) was unveiled in September of 2011. The SLS core architecture consists of a cryogenic first stage with five Space Shuttle Main Engines (SSMEs), and a cryogenic second stage using a new J-2X engine3. The baseline configuration employs two 5-segment solid rocket boosters to achieve a 70 metric ton payload capability, but a new, more capable booster system will be required to attain the goal of 130 metric tons to orbit. To this end, NASA s Marshall Space Flight Center recently released a NASA Research Announcement (NRA) entitled "Space Launch System (SLS) Advanced Booster Engineering Demonstration and/or Risk Reduction." The increased emphasis on affordability is evident in the language used in the NRA, which is focused on risk reduction "leading to an affordable Advanced Booster that meets the evolved capabilities of SLS" and "enabling competition" to "enhance SLS affordability. The purpose of the work presented in this paper is to perform an independent assessment of the elements that make up an affordable and realistic path forward for the SLS booster system, utilizing advanced design methods and technology evaluation techniques. The goal is to identify elements that will enable a more sustainable development program by exploring the trade space of heavy lift booster systems and focusing on affordability, operability, and reliability at the system and subsystem levels5. For this study

  4. Pakistan launches media blitz on AIDS.

    Science.gov (United States)

    Lynn, W

    1994-01-01

    In March 1994, the National AIDS Prevention and Control Programme in Pakistan launched its media campaign. Staffers have had to work within Islamic principles to inform the public about the risk of HIV infection and to encourage the public to adopt behavior to prevent its transmission. The media messages are not sexually explicit. They call for Pakistanis to call a hotline for or to ask medical professionals about more detailed information on AIDS. The hotline number is memorable (123). The 2 hotlines in Islamabad receive 250-300 calls/day. These hotlines deliver a recorded message with information on the significance of condoms in AIDS prevention and allows callers an opportunity to leave a telephone number or address if they want information. Staff advise callers who are concerned that they may be infected with HIV to obtain a test at 1 of 30 sites and to attend the National Institute for Health in Islamabad for more testing and counseling if the first test is positive. The hotline system will soon expand to all other major Pakistani cities. The program receives 300-400 letters/week asking for specific information. The program had workshops for journalists as its first wave of increasing AIDS awareness. The journalists followed with thoughtful articles on AIDS. Program staff spent much energy to obtain support from Islamic leaders. More media professionals have joined efforts to disseminate information through various media forums to encourage people to seek treatment for sexually transmitted diseases. The program's goal is a 55% increase in the number of people who can name at least 2 correct ways to prevent HIV transmission and an increase in condom use from 1% to 70%. The program eventually would like to increase outreach efforts by working with nongovernmental organizations and by developing videos and short stories.

  5. Flight Record Of The Long March Series Of Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Ying

    2009-01-01

    @@ (Continued) The 27th launch On August 9, 1992, a LM-2D launch vehicle sent the 13th recoverable satellite into space. The satellite operated in orbit for 16 days, fulfilled missions of scientific exploration and technical experiment and returned on August 25.

  6. China Plans to Launch FY-3 Meteorological Satellite in 2006

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    China's new generation polar orbit weather satellite FY-3 will be launched by LM-4B launch vehicle in 2006. The FY-3 would be equipped with new global, all-weather, multi-spectral, threedimensional sensors. The new satellite, an improved version of the FY-1, has the resolution of 250m and

  7. CGWIC S gned The Contract for Launching APStar 6B

    Institute of Scientific and Technical Information of China (English)

    SunQing

    2005-01-01

    Following the successful launch of APStar 6 on April 12, 2005,China Great Wall Industry Corporation (CGWIC), as the general contractor, will provide APStar 6B satellite and launch service with the LM-3B rocket for APT Satellite Holdings Ltd., Hong Kong (APT)

  8. Flight Record Of Long March Series Of Launch Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Ying

    2008-01-01

    @@ (Continued) The 7th Launch On September 9,1982,a LM-2C launch vehicle sent the 4th recoverable satellite,FSW-4 into space.The satellite returned to Earth on September 14 after it fulfilled its mission for scientific research and tests during 5-day operation in space.

  9. A Reference Model for Virtual Machine Launching Overhead

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Hao; Ren, Shangping; Garzoglio, Gabriele; Timm, Steven; Bernabeu, Gerard; Chadwick, Keith; Noh, Seo-Young

    2016-07-01

    Cloud bursting is one of the key research topics in the cloud computing communities. A well designed cloud bursting module enables private clouds to automatically launch virtual machines (VMs) to public clouds when more resources are needed. One of the main challenges in developing a cloud bursting module is to decide when and where to launch a VM so that all resources are most effectively and efficiently utilized and the system performance is optimized. However, based on system operational data obtained from FermiCloud, a private cloud developed by the Fermi National Accelerator Laboratory for scientific workflows, the VM launching overhead is not a constant. It varies with physical resource utilization, such as CPU and I/O device utilizations, at the time when a VM is launched. Hence, to make judicious decisions as to when and where a VM should be launched, a VM launching overhead reference model is needed. In this paper, we first develop a VM launching overhead reference model based on operational data we have obtained on FermiCloud. Second, we apply the developed reference model on FermiCloud and compare calculated VM launching overhead values based on the model with measured overhead values on FermiCloud. Our empirical results on FermiCloud indicate that the developed reference model is accurate. We believe, with the guidance of the developed reference model, efficient resource allocation algorithms can be developed for cloud bursting process to minimize the operational cost and resource waste.

  10. SAS Launches Biometric Identification at Airports All Over Sweden

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Scandinavian Airlines is to launch a new biometric identification system throughout Sweden.When traveling, your fingerprint will be matched to your check-in baggage. This makes the check-in process easier and improves security. The new technology will be launched during November and December at almost all airports served by Scandinavian Airlines in Sweden.

  11. Space Acquisitions: GAO Assessment of DOD Responsive Launch Report

    Science.gov (United States)

    2015-10-29

    number of satellites may be needed to provide the same level of capability, and the transition from existing system designs could increase costs...Also, DOD currently lacks requirements for responsive launch, but plans to validate future responsive launch requirements as it gains knowledge about...Programs Congressional Relations Public Affairs Please Print on Recycled Paper.

  12. STS-112 M.S. Magnus suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. --STS-112 Mission Specialist Sandra Magnus dons her space helmet for a final fit check in preparation for her launch to the International Space Station aboard Atlantis. Launch is scheduled for Oct. 2 between 2 and 6 p.m. EDT.

  13. 77 FR 50956 - Exclusion of Tethered Launches From Licensing Requirements

    Science.gov (United States)

    2012-08-23

    ... strength properties, (2) minimum factor of safety, (3) launch vehicle constraint, (4) no damage displayed... activities, while limiting the vehicle to low altitudes and thereby reducing the risk to the public. When the... thermal damage due to a launch vehicle's exhaust. This requirement would mitigate the risk of a tether...

  14. Effects of Capsaicin on Proliferation,Migration and Invasion of Human Large Cell Carcinoma NCI-H460%辣椒素对人大细胞肺癌细胞NCI-H460增殖和迁移与侵袭能力的影响

    Institute of Scientific and Technical Information of China (English)

    任公平; 那辉; 吕正鑫; 孙琳林

    2016-01-01

    Objective To investigate effects of capsaicin on proliferation,migration and invasion of human large cell carcinoma NCI-H460 and discuss the possible mechanisms by which capsaicin inhibits non-small cell lung cancer. Methods NCI-H460 cells were cultured in vitro and treated with capsaicin at various concentrations. Colony formation assay,wound healing assay,and transwell chamber invasion assay were used to detect the effects of capsaicin on proliferation,migration and invasion of NCI-H460 cells. Western blotting was used to detect the protein expression of E-cadherin,N-cadherin,Vimentin and Snail. Results The rate of colony formation of 100,200,300 μmol.L-1 of capsaicin were (91.17±24.38)%,(43.22±12.91)% and (15.71±4.26)%,respectively,the control group was (99.53±21.25)%.the rate of migration of NCI-H460 cells of 25,50, 100 μmol.L-1 of capsaicin were (85.83±17.43)%,(37.92±10.16)% and(21.08±6.39)%,respectively,the control group was (93.04±20.23)%.The number of invasion of NCI-H460 cells of 25,50,100 μmol.L-1 of capsaicin were (99±18.2),(75± 17.9) and( 52 ± 14. 1 ) , respectively, the control group was ( 107 ± 20. 4 ) . The middle and high-dose capsaicincould inhibit proliferation,migration and invasion of NCI-H460 cells ( P<0.05) ,and E-cadherin expression level was significantly up-regulated and N-cadherin, Vimentin, Snail expression level was significantly down-regulated ( P<0. 05 ) . Conclusion Capsaicin can inhibit proliferation,migration and invasion of NCI-H460 cells,the mechanism may be related to up-regulation of E-cadherin and down-regulation of N-cadherin,Vimentin and Snail expression levels.%目的:观察辣椒素对人大细胞肺癌细胞NCI-H460增殖、迁移与侵袭能力的影响,探讨其抗非小细胞肺癌的可能机制。方法体外培养NCI-H460细胞,以不同终浓度辣椒素进行处理。采用平板克隆形成实验、划痕修复实验、Transwell小室细胞侵袭实验,观察辣椒素对NCI-H460细胞增殖

  15. Distributed Web-Based Expert System for Launch Operations

    Science.gov (United States)

    Bardina, Jorge E.; Thirumalainambi, Rajkumar

    2005-01-01

    The simulation and modeling of launch operations is based on a representation of the organization of the operations suitable to experiment of the physical, procedural, software, hardware and psychological aspects of space flight operations. The virtual test bed consists of a weather expert system to advice on the effect of weather to the launch operations. It also simulates toxic gas dispersion model, and the risk impact on human health. Since all modeling and simulation is based on the internet, it could reduce the cost of operations of launch and range safety by conducting extensive research before a particular launch. Each model has an independent decision making module to derive the best decision for launch.

  16. Technique applied in electrical power distribution for Satellite Launch Vehicle

    Directory of Open Access Journals (Sweden)

    João Maurício Rosário

    2010-09-01

    Full Text Available The Satellite Launch Vehicle electrical network, which is currently being developed in Brazil, is sub-divided for analysis in the following parts: Service Electrical Network, Controlling Electrical Network, Safety Electrical Network and Telemetry Electrical Network. During the pre-launching and launching phases, these electrical networks are associated electrically and mechanically to the structure of the vehicle. In order to succeed in the integration of these electrical networks it is necessary to employ techniques of electrical power distribution, which are proper to Launch Vehicle systems. This work presents the most important techniques to be considered in the characterization of the electrical power supply applied to Launch Vehicle systems. Such techniques are primarily designed to allow the electrical networks, when submitted to the single-phase fault to ground, to be able of keeping the power supply to the loads.

  17. Identification of a radiosensitivity signature using integrative metaanalysis of published microarray data for NCI-60 cancer cells

    Directory of Open Access Journals (Sweden)

    Kim Han

    2012-07-01

    Full Text Available Abstract Background In the postgenome era, a prediction of response to treatment could lead to better dose selection for patients in radiotherapy. To identify a radiosensitive gene signature and elucidate related signaling pathways, four different microarray experiments were reanalyzed before radiotherapy. Results Radiosensitivity profiling data using clonogenic assay and gene expression profiling data from four published microarray platforms applied to NCI-60 cancer cell panel were used. The survival fraction at 2 Gy (SF2, range from 0 to 1 was calculated as a measure of radiosensitivity and a linear regression model was applied to identify genes or a gene set with a correlation between expression and radiosensitivity (SF2. Radiosensitivity signature genes were identified using significant analysis of microarrays (SAM and gene set analysis was performed using a global test using linear regression model. Using the radiation-related signaling pathway and identified genes, a genetic network was generated. According to SAM, 31 genes were identified as common to all the microarray platforms and therefore a common radiosensitivity signature. In gene set analysis, functions in the cell cycle, DNA replication, and cell junction, including adherence and gap junctions were related to radiosensitivity. The integrin, VEGF, MAPK, p53, JAK-STAT and Wnt signaling pathways were overrepresented in radiosensitivity. Significant genes including ACTN1, CCND1, HCLS1, ITGB5, PFN2, PTPRC, RAB13, and WAS, which are adhesion-related molecules that were identified by both SAM and gene set analysis, and showed interaction in the genetic network with the integrin signaling pathway. Conclusions Integration of four different microarray experiments and gene selection using gene set analysis discovered possible target genes and pathways relevant to radiosensitivity. Our results suggested that the identified genes are candidates for radiosensitivity biomarkers and that

  18. Breast cancer in women aging 35 years old and younger: The Egyptian National Cancer Institute (NCI) experience.

    Science.gov (United States)

    Darwish, A D; Helal, A M; Aly El-Din, N H; Solaiman, L L; Amin, A

    2017-02-01

    The aim is to identify the epidemiological and clinicopathological features associated with young breast cancer (BC) patients and to discuss factors affecting tumor recurrence and DFS. A retrospective analysis was conducted based on medical records from young females patients aged ≤35 years with pathologically confirmed primary breast cancer treated during 2008-2010 at NCI. Cases with non invasive cancer and non carcinoma histology are excluded. Of the 5408 cases diagnosed with breast cancer, 554 were young. Four hundred & fifty eight patients representing 9.2% were within our inclusion criteria. Almost half of the patients (45.9%) presented with stage III. Axillary nodes involvement was in 63.9%, 83.3% were grade 2. More than one quarter of tumors was hormone receptors negative (28.8%) & Her2 was over-expressed in 30%. Mastectomy was offered in 72% while conservative breast surgery in 26%, 69.2% received chemotherapy either adjuvant, neoadjuvant or both, 82.5% received adjuvant radiotherapy, 68.6% received hormonal therapy. Metastatic disease developed in 51.3%, with 31% having more than one site of metastases. After a median follow up period of 66 months, the median DFS of patients was 60 months. The median DFS was significantly shorter among patients with positive lymph nodes (P Breast cancer in young women is aggressive from the time of diagnosis. Our results provide baseline data of young BC in the Middle East & North Africa region; thus, contributing to future epidemiological and hospital-based researches. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Tendències de les publicacions informatives cientificomèdiques en l'era 2.0

    Directory of Open Access Journals (Sweden)

    González Pacanowski, Antonio

    2014-12-01

    Full Text Available En els últims anys ha crescut l'audiència que consulta continguts de salut en publicacions i mitjans a Internet, especialment a Europa i als Estats Units. S'ha passat d'un usuari d'Internet unidireccional en la comunicació a un escenari en el qual la multidireccionalitat i la instantaneïtat són constants. Els nous mitjans i la creació de plataformes d'intercanvi d'informació més especialitzada mostren que els recursos multimèdia i la interactivitat també poden donar-se en mitjans especialitzats i distants del públic general com ara informació sobre biomedicina i salut. Simultàniament, el canvi cap a actituds més solidàries i d'ajuda mútua aflora mitjançant les xarxes socials. Amb l'objectiu de traçar un escenari sobre el comportament de les audiències davant els continguts de caràcter científic, especialment els de tipus sanitari, s'analitzen en aquest treball els perfils i costums dels usuaris utilitzant referències estadístiques tant europees com nord-americanes. De la mateixa manera, s'identifica el mapa actual de cibermitjans relacionats amb la informació cientificomèdica segmentada en mitjans de comunicació generals, especialitzats i els propis del web 2.0, com ara blogs i mitjans cocreatius. Aquesta descripció permet orientar sobre les tendències que seguiran els públics diferents i segmentats, però ara interconnectats per les noves tecnologies, i sobre la transformació de l'arquitectura i les funcionalitats dels mitjans a Internet.En los últimos años ha crecido la audiencia que consulta contenidos de salud en publicaciones y medios en Internet, especialmente en Europa y Estados Unidos. Se ha pasado de un usuario de Internet unidireccional en la comunicación a un escenario en el que la multidireccionalidad y la instantaneidad son constantes. Los nuevos medios y la creación de plataformas de intercambio de información más especializada evidencian que los recursos multimedia y la interactividad tambi

  20. 8-溴-7-甲氧基白杨素诱导人小细胞肺癌NCI-H446细胞凋亡及其机制的探讨%Induction of apoptosis on human small cell lung cancer NCI-H446 cell line via increasing the ratio of Noxa to Mcl-1 by 8-bromo-7-methoxychrysin

    Institute of Scientific and Technical Information of China (English)

    邹敏; 安东建; 曹建国

    2009-01-01

    目的:观察8-溴-7-甲氧基白杨素(BrMChR)诱导人小细胞肺癌(NCI-H446)细胞凋亡作用,并探讨其与Noxa/Mcl-1比值增高的关系.方法:以体外培养的NCI-H446细胞和正常小鼠肺上皮CHL细胞为研究对象.MTT比色法测定NCI-H446细胞和CHL细胞活性;PI染色流式细胞术(FCM)分析NCI-H446细胞凋亡率;FITC标志间接免疫荧光FCM检测NCI-H446细胞NOXA, Mcl-1和Caspase-3蛋白表达水平.结果:MTT分析结果显示BrMChR明显抑制NCI-H446细胞活性,呈浓度依赖性,而对CHL细胞活性的抑制作用弱,其选择指数为46;PI染色FCM分析显示0.3、3.0和30.0 μmol/L的BrMChR作用于NCI-H446细胞48 h后,其凋亡率分别为8.83%、23.7%和34.9%, 其中BrMChR 3.0和30.0 μmol/L组较ChR 30.0 μmol/L (19.7%)高;间接免疫荧光FCM分析结果显示,凋亡相关基因Noxa表达上调和Mcl-1表达下调,Noxa/Mcl-1比值升高,Caspase-3活化.结论:BrMChR具有诱导人小细胞肺癌细胞凋亡的作用,其作用机制可能与其增高Noxa/Mcl-1比值,激活Caspase-3蛋白表达有关.

  1. Launching Astronomy: Standards and STEM Integration (LASSI)

    Science.gov (United States)

    French, Debbie; Burrows, Andrea C.; Myers, Adam D.

    2015-01-01

    While astronomy is prevalent in the Next Generation Science Standards, it is often relegated to the '4th nine-weeks' in middle and high school curricula. I.e., it is taught at the end of the year, if time permits. However, astronomy ties in many core ideas from chemistry, earth science, physics, and even biology (with astrobiology being an up-and-coming specialization) and mathematics. Recent missions to Mars have captured students' attention and have added excitement to the fields of engineering and technology. Using astronomy as a vehicle to teach science, technology, engineering, and mathematics (STEM) connects these disciplines in an engaging way. The workshop entitled, 'Launching Astronomy: Standards and STEM Integration,' (LASSI) is a year-long professional development (PD) opportunity for teachers in grades K-12 to use astronomy as a vehicle to teach STEM and implement science standards through astronomy. Eight teachers participated in a two-week summer workshop and six follow-up sessions are scheduled during the 2014-2015 school year. Additional teachers plan to participate in the upcoming follow-up sessions. We evaluate the effectiveness of the LASSI PD to identify and confront teachers' misconceptions in astronomy, and discuss whether teachers identified topics for which astronomy can be used as a vehicle for standards-based STEM curricula. Teachers from around Wyoming were invited to participate. Participating teachers were surveyed on the quality of the workshop, their astronomy content knowledge before and after listening to talks given by experts in the field, conducting standards-based inquiry activities, developing their own lessons, and their level of engagement throughout the workshop. Two-thirds of teachers planned to incorporate LASSI activities into their classrooms in this school year. Teachers' misconceptions and requests for astronomy-based curriculum were identified in the summer session. These will be addressed during the follow-up session

  2. Space Launch System Upper Stage Technology Assessment

    Science.gov (United States)

    Holladay, Jon; Hampton, Bryan; Monk, Timothy

    2014-01-01

    The Space Launch System (SLS) is envisioned as a heavy-lift vehicle that will provide the foundation for future beyond low-Earth orbit (LEO) exploration missions. Previous studies have been performed to determine the optimal configuration for the SLS and the applicability of commercial off-the-shelf in-space stages for Earth departure. Currently NASA is analyzing the concept of a Dual Use Upper Stage (DUUS) that will provide LEO insertion and Earth departure burns. This paper will explore candidate in-space stages based on the DUUS design for a wide range of beyond LEO missions. Mission payloads will range from small robotic systems up to human systems with deep space habitats and landers. Mission destinations will include cislunar space, Mars, Jupiter, and Saturn. Given these wide-ranging mission objectives, a vehicle-sizing tool has been developed to determine the size of an Earth departure stage based on the mission objectives. The tool calculates masses for all the major subsystems of the vehicle including propellant loads, avionics, power, engines, main propulsion system components, tanks, pressurization system and gases, primary structural elements, and secondary structural elements. The tool uses an iterative sizing algorithm to determine the resulting mass of the stage. Any input into one of the subsystem sizing routines or the mission parameters can be treated as a parametric sweep or as a distribution for use in Monte Carlo analysis. Taking these factors together allows for multi-variable, coupled analysis runs. To increase confidence in the tool, the results have been verified against two point-of-departure designs of the DUUS. The tool has also been verified against Apollo moon mission elements and other manned space systems. This paper will focus on trading key propulsion technologies including chemical, Nuclear Thermal Propulsion (NTP), and Solar Electric Propulsion (SEP). All of the key performance inputs and relationships will be presented and

  3. Vehicle Dynamics due to Magnetic Launch Propulsion

    Science.gov (United States)

    Galaboff, Zachary J.; Jacobs, William; West, Mark E.; Montenegro, Justino (Technical Monitor)

    2000-01-01

    The field of Magnetic Levitation Lind Propulsion (MagLev) has been around for over 30 years, primarily in high-speed rail service. In recent years, however, NASA has been looking closely at MagLev as a possible first stage propulsion system for spacecraft. This approach creates a variety of new problems that don't currently exist with the present MagLev trains around the world. NASA requires that a spacecraft of approximately 120,000 lbs be accelerated at two times the acceleration of gravity (2g's). This produces a greater demand on power over the normal MagLev trains that accelerate at around 0.1g. To be able to store and distribute up to 3,000 Mega Joules of energy in less than 10 seconds is a technical challenge. Another problem never addressed by the train industry and, peculiar only to NASA, is the control of a lifting body through the acceleration of and separation from the MagLev track. Very little is understood about how a lifting body will react with external forces, Such as wind gusts and ground effects, while being propelled along on soft springs such as magnetic levitators. Much study needs to be done to determine spacecraft control requirements as well as what control mechanisms and aero-surfaces should be placed on the carrier. Once the spacecraft has been propelled down the track another significant event takes place, the separation of the spacecraft from the carrier. The dynamics involved for both the carrier and the spacecraft are complex and coupled. Analysis of the reaction of the carrier after losing, a majority of its mass must be performed to insure control of the carrier is maintained and a safe separation of the spacecraft is achieved. The spacecraft angle of attack required for lift and how it will affect the carriage just prior to separation, along with the impacts of around effect and aerodynamic forces at ground level must be modeled and analyzed to define requirements on the launch vehicle design. Mechanisms, which can withstand the

  4. Study on Alternative Cargo Launch Options from the Lunar Surface

    Energy Technology Data Exchange (ETDEWEB)

    Cheryl A. Blomberg; Zamir A. Zulkefli; Spencer W. Rich; Steven D. Howe

    2013-07-01

    In the future, there will be a need for constant cargo launches from Earth to Mars in order to build, and then sustain, a Martian base. Currently, chemical rockets are used for space launches. These are expensive and heavy due to the amount of necessary propellant. Nuclear thermal rockets (NTRs) are the next step in rocket design. Another alternative is to create a launcher on the lunar surface that uses magnetic levitation to launch cargo to Mars in order to minimize the amount of necessary propellant per mission. This paper investigates using nuclear power for six different cargo launching alternatives, as well as the orbital mechanics involved in launching cargo to a Martian base from the moon. Each alternative is compared to the other alternative launchers, as well as compared to using an NTR instead. This comparison is done on the basis of mass that must be shipped from Earth, the amount of necessary propellant, and the number of equivalent NTR launches. Of the options, a lunar coil launcher had a ship mass that is 12.7% less than the next best option and 17 NTR equivalent launches, making it the best of the presented six options.

  5. Modeling the Virtual Machine Launching Overhead under Fermicloud

    Energy Technology Data Exchange (ETDEWEB)

    Garzoglio, Gabriele [Fermilab; Wu, Hao [Fermilab; Ren, Shangping [IIT, Chicago; Timm, Steven [Fermilab; Bernabeu, Gerard [Fermilab; Noh, Seo-Young [KISTI, Daejeon

    2014-11-12

    FermiCloud is a private cloud developed by the Fermi National Accelerator Laboratory for scientific workflows. The Cloud Bursting module of the FermiCloud enables the FermiCloud, when more computational resources are needed, to automatically launch virtual machines to available resources such as public clouds. One of the main challenges in developing the cloud bursting module is to decide when and where to launch a VM so that all resources are most effectively and efficiently utilized and the system performance is optimized. However, based on FermiCloud’s system operational data, the VM launching overhead is not a constant. It varies with physical resource (CPU, memory, I/O device) utilization at the time when a VM is launched. Hence, to make judicious decisions as to when and where a VM should be launched, a VM launch overhead reference model is needed. The paper is to develop a VM launch overhead reference model based on operational data we have obtained on FermiCloud and uses the reference model to guide the cloud bursting process.

  6. Micro, nano and pico satellites launched from the Romanian territory

    Science.gov (United States)

    Savu, G.

    2006-10-01

    In the frame of National Program "Aerospatial" The National Institute of Turbomachinery—COMOTI, Bucharest, Romania proposes a project of launching with minimum cost of microsatellites using the national territory. The geographical position of Romania is optimum for satellites launching due to the presence of the Black Sea in the eastern part of the country and due to its elongated shape, West-East, offering a launching surface of 1500 km (W-E) ×250km (N-S). Two modes of launching were analyzed: vertical, from the soil and horizontal, from a carrier aircraft. The second mode of launching doubtless has some advantages, particularly from the point of view of costs. It was analyzed the launching of a LEO satellite as a payload of a single stage rocket with solid propellant, launched from a fighter aircraft. The aerodynamic coefficients of the rocket, the equation of movement on the trajectory and the rocket engine thrust were calculated using a FORTRAN program—LSCS (language for the simulation the continuous systems). The shape of the trajectory was imposed (not optimized), finally resulting the performances, the main geometrical dimensions of the rocket and the mass of the satellite.

  7. Assessing Upper-Level Winds on Day-of-Launch

    Science.gov (United States)

    Bauman, William H., III; Wheeler, Mark M.

    2012-01-01

    On the day-or-launch. the 45th Weather Squadron Launch Weather Officers (LWOS) monitor the upper-level winds for their launch customers to include NASA's Launch Services Program (LSP). During launch operations, the payload launch team sometimes asks the LWO if they expect the upper level winds to change during the countdown but the LWOs did not have the capability to quickly retrieve or display the upper-level observations and compare them to the numerical weather prediction model point forecasts. The LWOs requested the Applied Meteorology Unit (AMU) develop a capability in the form of a graphical user interface (GUI) that would allow them to plot upper-level wind speed and direction observations from the Kennedy Space Center Doppler Radar Wind Profilers and Cape Canaveral Air Force Station rawinsondes and then overlay model point forecast profiles on the observation profiles to assess the performance of these models and graphically display them to the launch team. The AMU developed an Excel-based capability for the LWOs to assess the model forecast upper-level winds and compare them to observations. They did so by creating a GUI in Excel that allows the LWOs to first initialize the models by comparing the O-hour model forecasts to the observations and then to display model forecasts in 3-hour intervals from the current time through 12 hours.

  8. Inhibition of mitotic Aurora kinase A by alisertib induces apoptosis and autophagy of human gastric cancer AGS and NCI-N78 cells

    Directory of Open Access Journals (Sweden)

    Yuan CX

    2015-01-01

    Full Text Available Chun-Xiu Yuan,1,2 Zhi-Wei Zhou,2,3 Yin-Xue Yang,4 Zhi-Xu He,3 Xueji Zhang,5 Dong Wang,6 Tianxing Yang,7 Ning-Ju Wang,1 Ruan Jin Zhao,8 Shu-Feng Zhou21Department of Oncology, General Hospital Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 2Department of Pharmaceutical Science, College of Pharmacy, University of South Florida, Tampa, FL, USA; 3Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center and Sino–US Joint Laboratory for Medical Sciences, Guiyang Medical University, Guiyang, Guizhou, People’s Republic of China; 4Department of Colorectal Surgery, General Hospital, Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 5Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, People’s Republic of China; 6Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, People’s Republic of China; 7Department of Internal Medicine, University of Utah and Salt Lake Veterans Affairs Medical Center, Salt Lake City, UT, USA; 8Center for Traditional Chinese Medicine, Sarasota, FL, USAAbstract: Gastric cancer is one of the most common cancers and responds poorly to current chemotherapy. Alisertib (ALS is a second-generation, orally bioavailable, highly selective small-molecule inhibitor of the serine/threonine protein kinase Aurora kinase A (AURKA. ALS has been shown to have potent anticancer effects in preclinical and clinical studies, but its role in gastric cancer treatment is unclear. This study aimed to investigate the cancer cell-killing effect of ALS on gastric cancer cell lines AGS and NCI-N78, with a focus on cell proliferation, cell-cycle distribution, apoptosis, and autophagy and the mechanism of action. The results showed that ALS exhibited potent growth-inhibitory, proapoptotic, and proautophagic effects on AGS and

  9. STS-112 Commander Ashby suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-112 Commander Jeffrey Ashby finishes suiting up for launch. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  10. Adaptive Attitude Control of the Crew Launch Vehicle

    Science.gov (United States)

    Muse, Jonathan

    2010-01-01

    An H(sub infinity)-NMA architecture for the Crew Launch Vehicle was developed in a state feedback setting. The minimal complexity adaptive law was shown to improve base line performance relative to a performance metric based on Crew Launch Vehicle design requirements for all most all of the Worst-on-Worst dispersion cases. The adaptive law was able to maintain stability for some dispersions that are unstable with the nominal control law. Due to the nature of the H(sub infinity)-NMA architecture, the augmented adaptive control signal has low bandwidth which is a great benefit for a manned launch vehicle.

  11. STS-112 M.S. Magnus suits up before launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- STS-112 Mission Specialist Sandra Magnus finishes suiting up before launch. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  12. General Dynamic (GD) Launch Waveform On-Orbit Performance Report

    Science.gov (United States)

    Briones, Janette C.; Shalkhauser, Mary Jo

    2014-01-01

    The purpose of this report is to present the results from the GD SDR on-orbit performance testing using the launch waveform over TDRSS. The tests include the evaluation of well-tested waveform modes, the operation of RF links that are expected to have high margins, the verification of forward return link operation (including full duplex), the verification of non-coherent operational models, and the verification of radio at-launch operational frequencies. This report also outlines the launch waveform tests conducted and comparisons to the results obtained from ground testing.

  13. STS-112 M.S. Sellers suits up for launch

    Science.gov (United States)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - During suitup for launch, STS-112 Mission Specialist Piers Sellers smiles in anticipation of his first Shuttle flight. STS-112 is the 15th assembly flight to the International Space Station, carrying the S1 Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The CETA is the first of two human-powered carts that will ride along the ISS railway, providing mobile work platforms for future spacewalking astronauts. On the 11-day mission, three spacewalks are planned to attach the S1 truss to the Station. Launch is scheduled for 3:46 p.m. EDT from Launch Pad 39B.

  14. The NCI High Performance Computing (HPC) and High Performance Data (HPD) Platform to Support the Analysis of Petascale Environmental Data Collections

    Science.gov (United States)

    Evans, B. J. K.; Pugh, T.; Wyborn, L. A.; Porter, D.; Allen, C.; Smillie, J.; Antony, J.; Trenham, C.; Evans, B. J.; Beckett, D.; Erwin, T.; King, E.; Hodge, J.; Woodcock, R.; Fraser, R.; Lescinsky, D. T.

    2014-12-01

    The National Computational Infrastructure (NCI) has co-located a priority set of national data assets within a HPC research platform. This powerful in-situ computational platform has been created to help serve and analyse the massive amounts of data across the spectrum of environmental collections - in particular the climate, observational data and geoscientific domains. This paper examines the infrastructure, innovation and opportunity for this significant research platform. NCI currently manages nationally significant data collections (10+ PB) categorised as 1) earth system sciences, climate and weather model data assets and products, 2) earth and marine observations and products, 3) geosciences, 4) terrestrial ecosystem, 5) water management and hydrology, and 6) astronomy, social science and biosciences. The data is largely sourced from the NCI partners (who include the custodians of many of the national scientific records), major research communities, and collaborating overseas organisations. By co-locating these large valuable data assets, new opportunities have arisen by harmonising the data collections, making a powerful transdisciplinary research platformThe data is accessible within an integrated HPC-HPD environment - a 1.2 PFlop supercomputer (Raijin), a HPC class 3000 core OpenStack cloud system and several highly connected large scale and high-bandwidth Lustre filesystems. New scientific software, cloud-scale techniques, server-side visualisation and data services have been harnessed and integrated into the platform, so that analysis is performed seamlessly across the traditional boundaries of the underlying data domains. Characterisation of the techniques along with performance profiling ensures scalability of each software component, all of which can either be enhanced or replaced through future improvements. A Development-to-Operations (DevOps) framework has also been implemented to manage the scale of the software complexity alone. This ensures that

  15. Ressenya de la V Jornada de Criminologia «Les violències en l’àmbit familiar. Intervenció del sistema penal»

    Directory of Open Access Journals (Sweden)

    Estudis de Dret i Ciència Política

    2015-06-01

    Full Text Available

    El dia 5 de febrer de 2015 va tenir lloc a Barcelona la V Jornada de Criminologia organitzada per la UOC i pel Centre d’Estudis Jurídics i Formació Especialitzada (CEJFE de la Generalitat de Catalunya. El tema tractat en aquesta cinquena edició de la jornada, que es celebra anualment des de 2011, va ser «Les violències en l’àmbit familiar. Intervenció del sistema penal». 

  16. NCI will no longer support investigator-initiated phase III clinical trials through R01 and P01s grants | Division of Cancer Prevention

    Science.gov (United States)

    The NCI has traditionally provided support for all phases of clinical trials and interventions via grants and cooperative agreements (including the R03, R21, R01, P01, U01, U10, and UM1 mechanisms). Historically, the majority of early phase trials have been conducted under R03, R21, R01, P01, U01, and UM1 activity codes, whereas most Phase III clinical trials have been conducted under the U10 activity code, with a limited number of Phase III clinical trials performed under the R01, P01, and U01 activity codes... |

  17. Molecular mechanism of indirubin-3'-monoxime and Matrine in the reversal of paclitaxel resistance in NCI-H520/TAX25 cell line

    Institute of Scientific and Technical Information of China (English)

    LUO Su-xia; DENG Wen-ying; WANG Xin-feng; L(U) Hui-fang; HAN Li-li; CHEN Bei-bei; CHEN Xiao-bing

    2013-01-01

    Background Multidrug resistance (MDR) is a main reason for paclitaxel (TAX) treatment failure.Indirubin-3'-monoxime (IRO) and Matrine are traditional Chinese medicines,which may reverse the resistance of tumor cells to some chemotherapy drugs,but the relationship between paclitaxel resistance and Matrine is still unclear.The aim of this study was to explore the potential molecular mechanism of IRO and Matrine in reversal of TAX resistance.Methods In this study,MTT assay was used to measure the non-cytotoxic dosage of IRO and Matrine on NCI-H520/TAX25 cells and determine the reversal extent of TAX resistance under non-toxic doses.In addition,RT-PCR and Western blotting were used to evaluate the mRNA expression and the protein level of survivin,Oct-4,and Sox-2 in NCI-H520/FAX25 cells using semi-quantitative methods.Results There was no obvious inhibition on sensitive cell strains and drug-resistant strains,when the final concentration was at lest 4 μmol/L for IRO and 100 μmol/L for Matrine.So 4 μmol/L of IRO and 100 μmol/L of Matrine were considered as the reversal dosage.When 4 μmol/L of IRO or 100 μmol/L of Matrine were used together with TAX,the sensitivity to TAX increased evidently in NCI-H520/TAX2 cells; the reversal rate of IRO and Matrine was about 1.92 (43.56/22.6 nmol/L) and 1.74 (43.56/25.0 nmol/L),respectively.The mRNA expression and the protein level of survivin,Oct-4,and Sox-2 in NCI-H520/TAX25 decreased significantly (P <0.05) after addition of IRO or Matrine in TAX treatment,compared to that of TAX treatment alone.Conclusion The decrease in both mRNA expression and protein level of survivin,Oct-4,and Sox-2 might be the molecular mechanism,by which IRO and Matrine mediate the reversal of TAX resistance.

  18. Pensar el cos i practicar el cos : Construir una identitat pròpia a partir del cos i de les experiències vitals

    OpenAIRE

    Porzio, Laura

    2010-01-01

    (ES) En aquest treball teòric, metodològic i etnogràfic profund i intens, a través del relat biogràfic de l’Anna, skinhead girl antifeixista catalana, es reflexiona sobre els usos i significats del tatuatge com a pràctica corporal urbana i sobre la seva relació amb les experiències biogràfiques d’aquesta noia en el si d’identitats corporals individuals i col·lectives.

  19. 肺炎军团杆菌诱导NCI-H292细胞β-defensin-2表达的分子机制%Molecular mechanism of β-defensin-2 expression in Legionella pneumoniae induced NCI-H292 cells

    Institute of Scientific and Technical Information of China (English)

    佟爱华; 米运强; 李康; 李艳艳

    2012-01-01

    Objective To investigate the role of hBD-2 gene for the clinical anti - infective therapy. Methods Legionella pneumoniae infection in NCI - H292 cells, using colony formation assay, Western blotting , and ELISA to investgate the related gene changes in the MAPK pathway. Results hBD - 2 inhibited Legionella pneumoniae, and Legionella pneumoniae induced secretion of hBD - 2 mainly through the JNK and p38 pathway. Conclusions Legionella pneumoniae induces hBD - 2 secreted from NCI - H292 cells,which is mainly due to JNK and p38 pathway.%目的 探讨人β防御素2(human beta defensin 2,hBD-2)基因作用的分子机制,为临床抗感染治疗提供依据.方法 肺炎军团杆菌感染NCI-H292细胞,采用菌落形成实验,Western blot实验和ELISA实验检测丝裂原活化蛋白激酶(mitogen-activatived protein kinase,MAPK)途径内基因的变化.结果 hBD-2对肺炎军团杆菌有抑制作用,肺炎军团杆菌诱导分泌hBD-2主要是通过JNK和p38途径起作用.结论 肺炎军团杆菌主要通过c-Jun氨基末端激酶(the c-Jun N-terminal kinase,JNK)和p38途径诱导NCI-H292细胞分泌hBD-2.

  20. Analysis of Environmental Chemical Mixtures and Non-Hodgkin Lymphoma Risk in the NCI-SEER NHL Study

    Science.gov (United States)

    Czarnota, Jenna; Gennings, Chris; Colt, Joanne S.; De Roos, Anneclaire J.; Cerhan, James R.; Severson, Richard K.; Hartge, Patricia; Ward, Mary H.

    2015-01-01

    , Wheeler DC. 2015. Analysis of environmental chemical mixtures and non-Hodgkin lymphoma risk in the NCI-SEER NHL Study. Environ Health Perspect 123:965–970; http://dx.doi.org/10.1289/ehp.1408630 PMID:25748701

  1. D-558-2 launch and flight

    Science.gov (United States)

    1954-01-01

    (Bureau No. 37975 -- NACA 145). Skyrocket 143 flew all but one of its missions as part of the Douglas Aircraft Company contractor program to test the airplane's performance. NACA aircraft 143 was initially powered by a Westinghouse J34-40 turbojet engine configured only for ground takeoffs, but in 1954-55 the contractor modified it to an all-rocket air-launch capability featuring an LR8-RM-6, 4-chamber Reaction Motors engine rated at 6,000 pounds of thrust at sea level (the Navy designation for the Air Force LR-11 used in the X-1). In this configuration, NACA research pilot John McKay flew the airplane only once for familiarization on September 17, 1956. The 123 flights of NACA 143 served to validate wind-tunnel predictions of Skyrocket performance, except for the fact that the airplane experienced less drag above Mach 0.85 than the wind tunnels had indicated. NACA 144 also began its flight program with a turbojet powerplant. NACA pilots Robert A. Champine and John H. Griffith flew 21 times in this configuration to test airspeed calibrations and to research longitudinal and lateral stability and control. In the process, during August of 1949 they encountered pitchup problems, which NACA engineers recognized as serious because pitchups could produce a limiting and dangerous restriction on flight performance. Hence, they determined to make a complete investigation of the problem. In 1950 Douglas Aircraft Company replaced the turbojet with an LR-8 rocket engine, and its pilot, William B. Bridgeman, flew the aircraft seven times -- up to a speed of Mach 1.88 (1.88 times the speed of sound) and an altitude of 79,494 feet (the latter an unofficial world altitude record at the time, achieved on August 15, 1951). In the rocket configuration, a Navy P2B (Navy version of the B-29) launched the airplane at an altitude of approximately 30,000 feet after taking off from the ground with the Skyrocket attached beneath its bomb bay. During Bridgeman's supersonic flights, he encountered a

  2. Launch Vehicle Design Process Characterization Enables Design/Project Tool

    Science.gov (United States)

    Blair, J. C.; Ryan, R. S.; Schutzenhofer, L. A.; Robinson, Nancy (Technical Monitor)

    2001-01-01

    The objectives of the project described in this viewgraph presentation included the following: (1) Provide an overview characterization of the launch vehicle design process; and (2) Delineate design/project tool to identify, document, and track pertinent data.

  3. Launching the CUSBEA Article Series in SCIENCE CHINA Life Sciences

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    As a CUSBEA (China-United States Biochemistry Examination and Administration) Program fellow of Class IV (1985), I am very excited to announce the official launch of the CUSBEA Article Series in SCIENCE CHINA Life

  4. Fiber Optic Sensing Systems for Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The FOSS project primary test objectives are to demonstrate by flying on an Antares launch vehicle, the ability of FOSS flight hardware to measure strain and...

  5. Relationship of Worldwide Rocket Launch Crashes with Geophysical Parameters

    Directory of Open Access Journals (Sweden)

    N. Romanova

    2013-01-01

    Full Text Available A statistical comparison of launch crashes at different worldwide space ports with geophysical factors has been performed. A comprehensive database has been compiled, which includes 50 years of information from the beginning of the space age in 1957 about launch crashes occurring world-wide. Special attention has been paid to statistics concerning launches at the largest space ports: Plesetsk, Baikonur, Cape Canaveral, and Vandenberg. In search of a possible influence of geophysical factors on launch failures, such parameters as the vehicle type, local time, season, sunspot number, high-energy electron fluxes, and solar proton events have been examined. Also, we have analyzed correlations with the geomagnetic indices as indirect indicators of the space weather condition. Regularities found in this study suggest that further detailed studies of space weather effects on launcher systems, especially in the high-latitude regions, should be performed.

  6. STS-93 Pilot Ashby suits up for launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building during final launch preparations for the third time, STS-93 Pilot Jeffrey S. Ashby pulls on his glove, part of his launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Eileen Collins, Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  7. Risk Considerations of Bird Strikes to Space Launch Vehicles

    Science.gov (United States)

    Hales, Christy; Ring, Robert

    2016-01-01

    Within seconds after liftoff of the Space Shuttle during mission STS-114, a turkey vulture impacted the vehicle's external tank. The contact caused no apparent damage to the Shuttle, but the incident led NASA to consider the potential consequences of bird strikes during a Shuttle launch. The environment at Kennedy Space Center provides unique bird strike challenges due to the Merritt Island National Wildlife Refuge and the Atlantic Flyway bird migration routes. NASA is currently refining risk assessment estimates for the probability of bird strike to space launch vehicles. This paper presents an approach for analyzing the risks of bird strikes to space launch vehicles and presents an example. The migration routes, types of birds present, altitudes of those birds, exposed area of the launch vehicle, and its capability to withstand impacts affect the risk due to bird strike. A summary of significant risk contributors is discussed.

  8. Nytrox Oxidizers for NanoSat Launch Vehicles Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Space Propulsion Group, Inc. proposes to conduct systems studies to quantify the performance and cost advantages of Nytrox oxidizers for small launch vehicles. This...

  9. Platform Independent Launch Vehicle Avionics with GPS Metric Tracking Project

    Data.gov (United States)

    National Aeronautics and Space Administration — For this award, Tyvak proposes to develop a complete suite of avionics for a Nano-Launch Vehicle (NLV) based on the architecture determinations performed during...

  10. Electromagnetic Cavity Effects from Transmitters Inside a Launch Vehicle Fairing

    Science.gov (United States)

    Trout, Dawn H.; Wahid, Parveen F.; Stanley, James E.

    2009-01-01

    This paper provides insight into the difficult analytical issue for launch vehicles and spacecraft that has applicability outside of the launch industry. Radiation from spacecraft or launch vehicle antennas located within enclosures in the launch vehicle generates an electromagnetic environment that is difficult to accurately predict. This paper discusses the test results of power levels produced by a transmitter within a representative scaled vehicle fairing model and provides preliminary modeling results at the low end of the frequency test range using a commercial tool. Initially, the walls of the fairing are aluminum and later, layered with materials to simulate acoustic blanketing structures that are typical in payload fairings. The effects of these blanketing materials on the power levels within the fairing are examined.

  11. China-Japan-Korea Industrial Fair 2006 Launched

    Institute of Scientific and Technical Information of China (English)

    Zhang Yue

    2006-01-01

    @@ China-Japan-Korea Industrial Fair 2006 was launched in Qingdao city from March 20 to 23, Shandong province, according to the press release by China Council for the Promotion of International Trade(CCPIT).

  12. Modeling and Simulation of Shuttle Launch and Range Operations

    Science.gov (United States)

    Bardina, Jorge; Thirumalainambi, Rajkumar

    2004-01-01

    The simulation and modeling test bed is based on a mockup of a space flight operations control suitable to experiment physical, procedural, software, hardware and psychological aspects of space flight operations. The test bed consists of a weather expert system to advise on the effect of weather to the launch operations. It also simulates toxic gas dispersion model, impact of human health risk, debris dispersion model in 3D visualization. Since all modeling and simulation is based on the internet, it could reduce the cost of operations of launch and range safety by conducting extensive research before a particular launch. Each model has an independent decision making module to derive the best decision for launch.

  13. NE·TIGER Launches First Chinese Luxury Brand in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    November 6, 2010: Top Chinese luxury brand NE.TIGER launched a grand opening ceremony for its image store in Shanghai. The new store is located at Libao Square on Middle Huaihai Road, which has a commer-

  14. Green Non-dyed Textile Innovation Alliance officially launched

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    June 29, the Green Non-dyed Textile Innovation Alliance was officially launched by the China Textile Information Center, Nationa Textile Product Development Center together with China Chemical Fiber Industry Association

  15. COLD-SAT orbital experiment configured for Altas launch

    Science.gov (United States)

    Schuster, J. R.; Bennett, F. O.; Wachter, J. P.

    1990-01-01

    A study was done of the feasibility of conducting liquid hydrogen orbital storage, acquisition, and transfer experiments aboard a spacecraft launched by a commercial Atlas launch vehicle. Three hydrogen tanks are mated to a spacecraft bus that is similar to that used for three-axis-controlled satellites. The bus provides power, communications, and attitude control along with acceleration levels ranging from 10 exp -6 to 10 exp -4 g. At launch, all the liquid hydrogen is contained in the largest tank, which has an insulation system designed for both space operation and the short-term launch pad and ascent environment. This tank is much lighter and lower in cost than a vacuum-jacketed design, and is made possible by the experiment tanking options available due to the hydrogen-fueled Centaur upper stage of the Atlas I.

  16. Launch of physics journals boosts open-access club

    CERN Multimedia

    2007-01-01

    "Open-access publisher BioMed Central is launching three new physics journals under the sister brand-name PhysMath Central. they will sit alongside the company's portfolio of 176 biomedical titles." (1/4 page)

  17. Engineering Next Generation Launch Systems for Supportability Project

    Data.gov (United States)

    National Aeronautics and Space Administration — In order to meet the challenges of high probability of mission success for space exploration, ground support system for various launch operations that responds...

  18. Research Advocacy at NCI

    Science.gov (United States)

    The patient perspective research advocates brings into NCI’s research enterprise helps to inform research focus and support the dissemination of results that lead to new and better cancer prevention, detection, and treatment methods.

  19. NCI SRK Award

    Science.gov (United States)

    The SRK Fellowship is a highly competitive, unpaid, and annual, one-year program that provides additional mentoring opportunities, networking, seminars, and workshops to help prepare NCI’s female postdoctoral fellows for the competitive nature of the job market and help them remain in a biomedical research career.

  20. Experimental study of the inhibitory effect of capsaicin on PGE2 concentration of IL-1βinduced NCI-H460 cells by downregulating COX-2 and mPGES-1%辣椒素通过下调 COX-2和 mPGES-1抑制 IL-1β诱导的NCI-H460细胞 PGE2含量的实验研究

    Institute of Scientific and Technical Information of China (English)

    任公平; 那辉; 佟雷; 李华洋

    2016-01-01

    目的:观察辣椒素对 IL-1β诱导的人肺腺癌 NCI-H460细胞 PGE2含量的影响,并且进一步观察其对 COX-2和mPGES-1的影响,探讨其抗非小细胞肺癌(NSCLC)的可能机制。方法体外培养 NCI-H460细胞,采用 MTT 比色分析法,观察辣椒素对 NCI-H460细胞增殖抑制作用,计算 IC50;采用 IL-1β刺激的方法构建炎症模型,ELISA 法检测辣椒素对 NCI-H460细胞 PGE2含量和 COX-2活性的影响;Western blot 法检测辣椒素对 NCI-H460细胞 COX-2、mPGES-1蛋白表达的影响;Real-time PCR 法检测辣椒素对 NCI-H460细胞 COX-2 mRNA 和 mPGES-1 mRNA 表达的影响。结果MTT 比色分析结果表明,辣椒素对 NCI-H460细胞增殖具有明显抑制作用,与对照组比较,差异具有统计学意义(P <0.05或 P <0.01)。辣椒素明显降低 NCI-H460细胞 COX-2活性和 PGE2浓度,而且明显降低 NCI-H460细胞 COX-2、mPGES-1蛋白及其 mRNA 的表达,且呈剂量依赖性,与模型组比较,差异具有统计学意义(P <0.05)。结论辣椒素通过降低 NCI-H460细胞 COX-2和 mPGES-1 mRNA 表达从而抑制 PGE2释放,可能是其抗NSCLC 的机制之一。%Objective To observe the effects of capsaicin on PGE2 concentration of IL-1β-induced human large cell carcinoma NCI-H460 cells,and further observe its effect on COX-2 and mPGES-1 so as to explore the possible mechanisms against non-small cell lung cancer.Methods NCI-H460 cells were cultured in vitro ;the effect of capsaicin in inhibiting NCI-H460 cells proliferation was observed.The 50% inhibitory concentration (IC50 ) was measured by MTT assay.IL-1βstimulation method was used to construct inflammation model,and the effects of capsaicin on COX-2 activity and PGE2 concentration in NCI-H460 cells were measured by ELISA.The effects of capsaicin on COX-2 and mPGES-1 protein level in NCI-H460 cells were analyzed by Western blot;the effects of capsaicin on COX-2 mRNA and mPGES-1 mRNA expressions in NCI-H460 cells were analyzed by Real

  1. The Global Entry of New Pharmaceuticals: A Joint Investigation of Launch Window and Price

    NARCIS (Netherlands)

    I.W.J. Verniers (Isabel); S. Stremersch (Stefan); C. Croux (Christophe)

    2011-01-01

    textabstractResearch on the launch of new products in the international realm is scarce. The present paper is the first to document how launch window (difference in months between the first worldwide launch and the subsequent launch in a specific country) and launch price are interrelated and how re

  2. Alternatives for Future U.S. Space-Launch Capabilities

    Science.gov (United States)

    2006-10-01

    as the crew carrier and the similarly derived top-mounted super-heavy launcher as the cargo carrier—that is, the shuttle-derived super-heavy alterna ...tive considered by CBO. Through 2017, CBO’s estimate of the budgetary resources needed to execute that alterna - tive on the more ambitious schedule...U.S. launch capacity exclude the Sea Launch system operated by Boeing in partnership with RSC- Energia (based in Moscow), Kvaerner ASA (based in Oslo

  3. Performing a Launch Depressurization Test on an Inflatable Space Habitat

    Science.gov (United States)

    Martin, Patrick J.; Van Velzer, Paul

    2014-01-01

    In July, 2014 JPL's Environmental Test Laboratory successfully performed a launch depressurization test on an inflatable space habitat proposed to be installed on the International Space Station. The inflatable habitat is to be launched in the SpaceX Dragon Trunk. During the launch, the unpressurized Dragon Trunk will rapidly change from ground level atmospheric pressure to the vacuum of space. Since the inflatable habitat is tightly folded during launch with multiple layers of bladder, Kevlar fabric sections, and micro-meteoroid shielding, it was not possible to analyze or simulate how the residual air pockets would behave during the launch. If the inflatable habitat does not vent adequately and expands, it could rupture the payload bay of the launch vehicle. A launch depressurization test was chosen as the best way to qualify the inflatable habitat. When stowed, the inflatable habitat measured approximately 241 cm (95 inches) in diameter by 152 cm (60 inches) high and weighed close to 1361 kg (3,000 pounds). Two vacuum chambers connected by a large vacuum line were used to perform this test. The inflatable habitat was mounted in the smaller chamber, which was 396 cm (13 feet) in diameter and 1128 cm (37 feet) high. The larger chamber, which was 823 cm (27 feet) in diameter and 2,591 cm (85 feet) high, was rough pumped and used as a vacuum reservoir. A two stage axial type compressor and ten Stokes vacuum pumps were also used during the depressurization. Opening a butterfly valve on the vacuum line, at the smaller chamber, was manually controlled so that the smaller chamber's depressurization rate matched the launch pressure profile.

  4. Apollo 11 astronaut Neil Armstrong suits up before launch

    Science.gov (United States)

    1969-01-01

    Apollo 11 Commander Neil Armstrong prepares to put on his helmet with the assistance of a spacesuit technician during suiting operations in the Manned Spacecraft Operations Building (MSOB) prior to the astronauts' departure to Launch Pad 39A. The three astronauts, Edwin E. Aldrin Jr., Neil A Armstrong and Michael Collins, will then board the Saturn V launch vehicle, scheduled for a 9:32 a.m. EDT liftoff, for the first manned lunar landing mission.

  5. Apollo 11 Cmdr Neil Armstrong watches STS-83 launch

    Science.gov (United States)

    1997-01-01

    Apollo 11 Commander Neil A. Armstrong and his wife, Carol, were among the many special NASA STS-83 launch guests who witnessed the liftoff of the Space Shuttle Columbia April 4 at the Banana Creek VIP Viewing Site at KSC. Columbia took off from Launch Pad 39A at 2:20:32 p.m. EST to begin the 16-day Microgravity Science Laboratory-1 (MSL-1) mission.

  6. Optimization and Sensitivity Analysis for a Launch Trajectory

    Science.gov (United States)

    2014-12-01

    Finding solutions to a boundary value problem can be time consuming and difficult due to the twin curses of sensitivity and dimensionality. In an...Hamiltonian boundary value problem. Finding solutions to a boundary value problem can be time consuming and difficult due to the twin curses of...case performance to be identified. This knowledge will lead to more flexibility in the launch window and a more reliable launch trajectory. D

  7. Shape Memory Alloy (SMA)-Based Launch Lock

    Science.gov (United States)

    Badescu, Mircea; Bao, Xiaoqi; Bar-Cohen, Yoseph

    2014-01-01

    Most NASA missions require the use of a launch lock for securing moving components during the launch or securing the payload before release. A launch lock is a device used to prevent unwanted motion and secure the controlled components. The current launch locks are based on pyrotechnic, electro mechanically or NiTi driven pin pullers and they are mostly one time use mechanisms that are usually bulky and involve a relatively high mass. Generally, the use of piezoelectric actuation provides high precession nanometer accuracy but it relies on friction to generate displacement. During launch, the generated vibrations can release the normal force between the actuator components allowing shaft's free motion which could result in damage to the actuated structures or instruments. This problem is common to other linear actuators that consist of a ball screw mechanism. The authors are exploring the development of a novel launch lock mechanism that is activated by a shape memory alloy (SMA) material ring, a rigid element and an SMA ring holding flexure. The proposed design and analytical model will be described and discussed in this paper.

  8. Shape Memory Alloy (SMA)-Based Launch Lock

    Science.gov (United States)

    Badescu, Mircea; Bao, Xiaoqi; Bar-Cohen, Yoseph

    2014-01-01

    Most NASA missions require the use of a launch lock for securing moving components during the launch or securing the payload before release. A launch lock is a device used to prevent unwanted motion and secure the controlled components. The current launch locks are based on pyrotechnic, electro mechanically or NiTi driven pin pullers and they are mostly one time use mechanisms that are usually bulky and involve a relatively high mass. Generally, the use of piezoelectric actuation provides high precession nanometer accuracy but it relies on friction to generate displacement. During launch, the generated vibrations can release the normal force between the actuator components allowing shaft's free motion which could result in damage to the actuated structures or instruments. This problem is common to other linear actuators that consist of a ball screw mechanism. The authors are exploring the development of a novel launch lock mechanism that is activated by a shape memory alloy (SMA) material ring, a rigid element and an SMA ring holding flexure. The proposed design and analytical model will be described and discussed in this paper.

  9. Proceedings of the heavy lift launch vehicle tropospheric effects workshop

    Energy Technology Data Exchange (ETDEWEB)

    1979-12-01

    A workshop, sponsored by the Argonne National Laboratory, on Heavy Lift Launch Vehicle (HLLV) troposheric effects was held in Chicago, Illinois, on September 12, 13, and 14, 1978. Briefings were conducted on the latest HLLV congigurations, launch schedules, and proposed fuels. The geographical, environmental, and ecological background of three proposed launch sites were presented in brief. The sites discussed were launch pads near the Kennedy Space Center (KSC), a site in the southwestern United States near Animus, New Mexico, and an ocean site just north of the equator off the coast of Ecuador. A review of past efforts in atmospheric dynamics modeling, source term prediction, atmospheric effects, cloud rise modeling, and rainout/washout effects for the Space Shuttle tropospheric effects indicated that much of the progress made in these areas has direct applicability to the HLLV. The potential pollutants from the HLLV are different and their chymical interactions with the atmosphere are more complex, but the analytical techniques developed for the Space Shuttle can be applied, with the appropriate modification, to the HLLV. Reviews were presented of the ecological baseline monitoring being performed at KSC and the plant toxicology studies being conducted at North Carolina State. Based on the proposed launch sites, the latest HLLV configuration fuel, and launch schedule, the attendees developed a lit of possible environmental issues associated with the HLLV. In addition, a list of specific recommendations for short- and long-term research to investigate, understand, and possibly mitigate the HLLV environmental impacts was developed.

  10. The DARPA/USAF Falcon Program Small Launch Vehicles

    Science.gov (United States)

    Weeks, David J.; Walker, Steven H.; Thompson, Tim L.; Sackheim, Robert; London, John R., III

    2006-01-01

    Earlier in this decade, the U.S. Air Force Space Command and the Defense Advanced Research Projects Agency (DARPA), in recognizing the need for low-cost responsive small launch vehicles, decided to partner in addressing this national shortcoming. Later, the National Aeronautics and Space Administration (NASA) joined in supporting this effort, dubbed the Falcon Program. The objectives of the Small Launch Vehicle (SLV) element of the DARPA/USAF Falcon Program include the development of a low-cost small launch vehicle(s) that demonstrates responsive launch and has the potential for achieving a per mission cost of less than $5M when based on 20 launches per year for 10 years. This vehicle class can lift 1000 to 2000 lbm payloads to a reference low earth orbit. Responsive operations include launching the rocket within 48 hours of call up. A history of the program and the current status will be discussed with an emphasis on the potential impact on small satellites.

  11. Human Performance Modeling and Simulation for Launch Team Applications

    Science.gov (United States)

    Peaden, Cary J.; Payne, Stephen J.; Hoblitzell, Richard M., Jr.; Chandler, Faith T.; LaVine, Nils D.; Bagnall, Timothy M.

    2006-01-01

    This paper describes ongoing research into modeling and simulation of humans for launch team analysis, training, and evaluation. The initial research is sponsored by the National Aeronautics and Space Administration's (NASA)'s Office of Safety and Mission Assurance (OSMA) and NASA's Exploration Program and is focused on current and future launch team operations at Kennedy Space Center (KSC). The paper begins with a description of existing KSC launch team environments and procedures. It then describes the goals of new Simulation and Analysis of Launch Teams (SALT) research. The majority of this paper describes products from the SALT team's initial proof-of-concept effort. These products include a nominal case task analysis and a discrete event model and simulation of launch team performance during the final phase of a shuttle countdown; and a first proof-of-concept training demonstration of launch team communications in which the computer plays most roles, and the trainee plays a role of the trainee's choice. This paper then describes possible next steps for the research team and provides conclusions. This research is expected to have significant value to NASA's Exploration Program.

  12. Acoustic and Vibration Environment for Crew Launch Vehicle Mobile Launcher

    Science.gov (United States)

    Vu, Bruce T.

    2007-01-01

    A launch-induced acoustic environment represents a dynamic load on the exposed facilities and ground support equipment (GSE) in the form of random pressures fluctuating around the ambient atmospheric pressure. In response to these fluctuating pressures, structural vibrations are generated and transmitted throughout the structure and to the equipment items supported by the structure. Certain equipment items are also excited by the direct acoustic input as well as by the vibration transmitted through the supporting structure. This paper presents the predicted acoustic and vibration environments induced by the launch of the Crew Launch Vehicle (CLV) from Launch Complex (LC) 39. The predicted acoustic environment depicted in this paper was calculated by scaling the statistically processed measured data available from Saturn V launches to the anticipated environment of the CLV launch. The scaling was accomplished by using the 5-segment Solid Rocket Booster (SRB) engine parameters. Derivation of vibration environment for various Mobile Launcher (ML) structures throughout the base and tower was accomplished by scaling the Saturn V vibration environment.

  13. Optimal control theory determination of feasible return-to-launch-site aborts for the HL-20 Personnel Launch System vehicle

    Science.gov (United States)

    Dutton, Kevin E.

    1994-01-01

    The personnel launch system (PLS) being studied by NASA is a system to complement the space shuttle and provide alternative access to space. The PLS consists of a manned spacecraft launched by an expendable launch vehicle (ELV). A candidate for the manned spacecraft is the HL-20 lifting body. In the event of an ELV malfunction during the initial portion of the ascent trajectory, the HL-20 will separate from the rocket and perform an unpowered return to launch site (RTLS) abort. This work details an investigation, using optimal control theory, of the RTLS abort scenario. The objective of the optimization was to maximize final altitude. With final altitude as the cost function, the feasibility of an RTLS abort at different times during the ascent was determined. The method of differential inclusions was used to determine the optimal state trajectories, and the optimal controls were then calculated from the optimal states and state rates.

  14. Virtual screening of human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase against the NCI diversity set by use of AutoDock to identify novel nonfolate inhibitors.

    Science.gov (United States)

    Li, Chenglong; Xu, Lan; Wolan, Dennis W; Wilson, Ian A; Olson, Arthur J

    2004-12-30

    AICAR transformylase (5-aminoimidazole-4-carboxamide ribonucleotide transformylase) is a folate-dependent activity of the bifunctional protein ATIC (AICAR transformylase and IMP cyclohydrolase) and is responsible for catalyzing the penultimate step of the de novo purine biosynthetic pathway. As such, AICAR transformylase has been proposed as a potential target for antineoplastic drug design. Virtual screening of the human AICAR transformylase active site by use of AutoDock against the NCI diversity set, a library of compounds with nonredundant pharmacophore profiles, has revealed 44 potential inhibitor candidates. In vitro inhibition assay of 16 soluble compounds from this list revealed that eight compounds with novel scaffolds, relative to the general folate template, had micromolar inhibition. Subsequent extension of docking trials on compounds with similar scaffolds from the entire NCI-3D database has unveiled 11 additional inhibitors that were confirmed by the in vitro inhibition assay. In particular, one compound, NSC30171, had nanomolar inhibition (K(i) = 154 nM, IC(50) = 600 nM) against AICAR transformylase. These 19 inhibitors serve as novel templates/scaffolds for development of more potent and specific non-folate-based AICAR transformylase inhibitors.

  15. La colección ibero-balear de Meloidae Gyllenhal, 1810 (Coleoptera, Tenebrionoidea del Museu de Ciències Naturals de Barcelona

    Directory of Open Access Journals (Sweden)

    Prieto, M.

    2016-12-01

    Full Text Available The Ibero-Balearic collection of Meloidae Gyllenhal, 1810 (Coleoptera, Tenebrionoidea of the Museu de Ciències Naturals de Barcelona A commented catalogue of the Ibero-Balearic collection of Meloidae Gyllenhal, 1810 housed in the Museu de Ciències Naturals de Barcelona is presented. The studied material consists of 2,129 specimens belonging to 49 of 64 species from the Iberian peninsula and the Balearic Islands. The temporal coverage of the collection extends from the last decades of the nineteenth century to the present time. Revision, documentation, and computerization of the material have been made, resulting in 963 collection records (June 2014. For each lot, the catalogue includes the register number, geographical data, collection date, collector or origin of the collection, and number of specimens. Information about taxonomy and distribution of the species is also given. Chorological novelties are provided, extending the distribution areas for most species. The importance of the collection for the knowledge of the Ibero-Balearic fauna of Meloidae is discussed, particularly concerning the area of Catalonia (northeastern Iberian peninsula as it accounts for 60% of the records. Some rare or particularly interesting species in the collection are highlighted, as are those requiring protection measures in Spain and Catalonia. The catalogue also shows a brief gallery of photographs that includes four type specimens.

  16. From mice and men to earth and space: joint NASA-NCI workshop on lung cancer risk resulting from space and terrestrial radiation.

    Science.gov (United States)

    Shay, Jerry W; Cucinotta, Francis A; Sulzman, Frank M; Coleman, C Norman; Minna, John D

    2011-11-15

    On June 27-28, 2011, scientists from the National Cancer Institute (NCI), NASA, and academia met in Bethesda to discuss major lung cancer issues confronting each organization. For NASA, available data suggest that lung cancer is the largest potential cancer risk from space travel for both men and women and quantitative risk assessment information for mission planning is needed. In space, the radiation risk is from high energy and charge (HZE) nuclei (such as Fe) and high-energy protons from solar flares and not from gamma radiation. In contrast, the NCI is endeavoring to estimate the increased lung cancer risk from the potential widespread implementation of computed tomographic (CT) screening in individuals at high risk for developing lung cancer based on the National Lung Cancer Screening Trial (NLST). For the latter, exposure will be X-rays from CT scans from the screening (which uses "low-dose" CT scans) and also from follow-up scans used to evaluate abnormalities found during initial screening. Topics discussed included the risk of lung cancer arising after HZE particle, proton, and low-dose exposure to Earth's radiation. The workshop examined preclinical models, epidemiology, molecular markers, "omics" technology, radiobiology issues, and lung stem cells that relate to the development of lung cancer.

  17. NCI in vitro and in silico anticancer screen, cell cycle pertubation and apoptosis-inducing potential of new acylated, benzylidene and isopropylidene derivatives of andrographolide.

    Science.gov (United States)

    Wong, Charng Choon; Sagineedu, Sreenivasa Rao; Sumon, Shariful Hasan; Sidik, Shiran Mohamad; Phillips, Roger; Lajis, Nordin H; Stanslas, Johnson

    2014-09-01

    Andrographolide (AGP) is the main bioactive constituent isolated from the traditional medicinal, Andrographis paniculata which contributes towards its various biological activities, including anticancer property. In this study, a series of new AGP derivatives were semi-synthesised and screened against the NCI in vitro 60 cell lines. From the screening results, we had identified SRS07 as the most potent AGP derivative, against breast and colon cancer cell lines. Subsequently, SRS07 was tested for its capability to induce cell cycle arrest and apoptosis in MCF-7 and HCT116 cancer cells. SRS07 effectively induced G1 cell cycle arrest in both cell lines and ultimately apoptosis by inducing DNA fragmentation in HCT116 cells. The apoptotic cell death induced by SRS07 was confirmed via FITC Annexin-V double staining. Western blot analysis of SRS07-treated HCT116 cells revealed that the compound induced apoptosis be activating caspase 8 which in turn cleaved Bid to t-Bid to initiate cell death cascade. Prediction of the possible mode of action of SRS07 by utilising NCI COMPARE analysis failed to reveal a distinct mechanism category. Hence, it is speculated that SRS07 possesses novel mechanism of action. In conclusion, SRS07 demonstrated superior in vitro anticancer profiles and emerged as a potential lead anticancer candidate.

  18. Demethoxycurcumin alters gene expression associated with DNA damage, cell cycle and apoptosis in human lung cancer NCI-H460 cells in vitro.

    Science.gov (United States)

    Ko, Yang-Ching; Hsu, Shu-Chun; Liu, Hsin-Chung; Hsiao, Yung-Ting; Hsia, Te-Chun; Yang, Su-Tso; Hsu, Wu-Huei; Chung, Jing-Gung

    2015-01-01

    Lung cancer is the leading cause of cancer-related deaths and new lung cancer cases are continuously emerging around the globe; however, treatment of lung cancer remains unsatisfactory. Demethoxycurcumin (DMC) has been shown to exert cytotoxic effects in human cancer cells via induction of apoptosis. However, the effects of DMC on genetic mechanisms associated with these actions have not been yet elucidated. Human lung cancer NCI-H460 cells were incubated with or without 35 μM of DMC for 24 h and total RNA was extracted for cDNA synthesis labeling and microarray hybridization, followed by fluor-labeled cDNA hybridization on chip. Expression Console software with default Robust Multichip Analysis (RMA) parameters were used for detecting and quantitating the localized concentrations of fluorescent molecules. The GeneGo software was used for investigating key genes involved and their possible interaction pathways. Genes associated with DNA damage and repair, cell-cycle check point and apoptosis could be altered by DMC; in particular, 144 genes were found up-regulated and 179 genes down-regulated in NCI-H460 cells after exposure to DMC. In general, DMC-altered genes may offer information to understand the cytotoxic mechanism of this agent at the genetic level since gene alterations can be useful biomarkers or targets for the diagnosis and treatment of human lung cancer in the future.

  19. Estimation of the Mechanism of Adrenal Action of Endocrine-Disrupting Compounds Using a Computational Model of Adrenal Steroidogenesis in NCI-H295R Cells

    Directory of Open Access Journals (Sweden)

    Ryuta Saito

    2016-01-01

    Full Text Available Adrenal toxicity is one of the major concerns in drug development. To quantitatively understand the effect of endocrine-active compounds on adrenal steroidogenesis and to assess the human adrenal toxicity of novel pharmaceutical drugs, we developed a mathematical model of steroidogenesis in human adrenocortical carcinoma NCI-H295R cells. The model includes cellular proliferation, intracellular cholesterol translocation, diffusional transport of steroids, and metabolic pathways of adrenal steroidogenesis, which serially involve steroidogenic proteins and enzymes such as StAR, CYP11A1, CYP17A1, HSD3B2, CYP21A2, CYP11B1, CYP11B2, HSD17B3, and CYP19A1. It was reconstructed in an experimental dynamics of cholesterol and 14 steroids from an in vitro steroidogenesis assay using NCI-H295R cells. Results of dynamic sensitivity analysis suggested that HSD3B2 plays the most important role in the metabolic balance of adrenal steroidogenesis. Based on differential metabolic profiling of 12 steroid hormones and 11 adrenal toxic compounds, we could estimate which steroidogenic enzymes were affected in this mathematical model. In terms of adrenal steroidogenic inhibitors, the predicted action sites were approximately matched to reported target enzymes. Thus, our computer-aided system based on systems biological approach may be useful to understand the mechanism of action of endocrine-active compounds and to assess the human adrenal toxicity of novel pharmaceutical drugs.

  20. Estimation of the Mechanism of Adrenal Action of Endocrine-Disrupting Compounds Using a Computational Model of Adrenal Steroidogenesis in NCI-H295R Cells.

    Science.gov (United States)

    Saito, Ryuta; Terasaki, Natsuko; Yamazaki, Makoto; Masutomi, Naoya; Tsutsui, Naohisa; Okamoto, Masahiro

    2016-01-01

    Adrenal toxicity is one of the major concerns in drug development. To quantitatively understand the effect of endocrine-active compounds on adrenal steroidogenesis and to assess the human adrenal toxicity of novel pharmaceutical drugs, we developed a mathematical model of steroidogenesis in human adrenocortical carcinoma NCI-H295R cells. The model includes cellular proliferation, intracellular cholesterol translocation, diffusional transport of steroids, and metabolic pathways of adrenal steroidogenesis, which serially involve steroidogenic proteins and enzymes such as StAR, CYP11A1, CYP17A1, HSD3B2, CYP21A2, CYP11B1, CYP11B2, HSD17B3, and CYP19A1. It was reconstructed in an experimental dynamics of cholesterol and 14 steroids from an in vitro steroidogenesis assay using NCI-H295R cells. Results of dynamic sensitivity analysis suggested that HSD3B2 plays the most important role in the metabolic balance of adrenal steroidogenesis. Based on differential metabolic profiling of 12 steroid hormones and 11 adrenal toxic compounds, we could estimate which steroidogenic enzymes were affected in this mathematical model. In terms of adrenal steroidogenic inhibitors, the predicted action sites were approximately matched to reported target enzymes. Thus, our computer-aided system based on systems biological approach may be useful to understand the mechanism of action of endocrine-active compounds and to assess the human adrenal toxicity of novel pharmaceutical drugs.