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Sample records for nassau county ny

  1. Aerial Photography and Imagery, Ortho-Corrected - 2007 Digital Orthophotos - FDEM - Nassau County

    Data.gov (United States)

    NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Nassau County, Florida. An orthophoto is remotely sensed image data in...

  2. Aerial Photography and Imagery, Ortho-Corrected - 2008 Digital Orthophotos - Nassau County

    Data.gov (United States)

    NSGIC Education | GIS Inventory — This dataset is a collection of GeoTIFF and MrSID format natural color orthophotos covering Nassau County, Florida. An orthophoto is remotely sensed image data in...

  3. 2008 FEMA Lidar: South Oneida County (NY)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — For Oneida County, NY, there were two types of elevation datasets. The first type is LiDAR and the second one is Auto-correlation DEM. Auto-correlation DEM data was...

  4. Pipeline corridors through wetlands - impacts on plant communities: Deep Creek and Brandy Branch crossings, Nassau County, Florida

    Energy Technology Data Exchange (ETDEWEB)

    Shem, L.M.; Van Dyke, G.D.; Zimmerman, R.E.

    1994-12-01

    The goal of the Gas Research Institute Wetland Corridors Program is to document impacts of existing pipelines on the wetlands they traverse. To accomplish this goal, 12 existing wetland crossings were surveyed. These sites varied in elapsed time since pipeline construction, wetland type, pipeline installation techniques, and right-of-way (ROW) management practices. This report presents the results of surveys conducted July 14-18, 1992, at the Deep Creek and the Brandy Branch crossings of a pipeline installed during May 1991 in Nassau County, Florida. Both floodplains supported bottomland hardwood forests. The pipeline at the Deep Creek crossing was installed by means of horizontal directional drilling after the ROW had been clear-cut, while the pipeline at the Brandy Branch crossing was installed by means of conventional open trenching. Neither site was seeded or fertilized. At the time of sampling, a dense vegetative community, made up primarily of native perennial herbaceous species, occupied the ROW within the Deep Creek floodplain. The Brandy Branch ROW was vegetated by a less dense stand of primarily native perennial herbaceous plants. Plant diversity was also lower at the Brandy Branch crossing than at the Deep Creek crossing. The results suggest that some of the differences in plant communities are related to the more hydric conditions at the Brandy Branch floodplain.

  5. Aerial Photography and Imagery, Ortho-Corrected - 2010 NAIP Imagery - Nassau County

    Data.gov (United States)

    NSGIC Education | GIS Inventory — This data set contains polygons delineating the seams boundary between acquired imagery used in the creation of DOQQs and compressed county mosaic (CCM). The DOQQ...

  6. Coastal Analysis, Nassau,NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Coastal study as defined in FEMA Guides and Specifications, Appendix D: Guidance for coastal Flooding Analyses and Mapping submitted as a result of a coastal study....

  7. Hydrogeology and Extent of Saltwater Intrusion in the Northern Part of the Town of Oyster Bay, Nassau County, New York: 1995–98

    Science.gov (United States)

    Stumm, Frederick; Lange, Andrew D.; Candela, Jennifer L.

    2004-01-01

    The Oyster Bay study area, in the northern part of Nassau County, N.Y., is underlain by unconsolidated deposits that form a sequence of aquifers and confining units. At least one production well has been affected by the intrusion of saltwater from Hempstead Harbor, Long Island Sound, and Cold Spring Harbor. Nineteen boreholes were drilled during 1995-98 for the collection of hydrogeologic, geochemical, and geophysical data to delineate the subsurface geology and the extent of saltwater intrusion. Continuous high-resolution marine-seismic-reflection surveys in the surrounding embayments of the Oyster Bay study area were conducted in 1996.New drill-core data indicate two hydrogeologic units—the North Shore aquifer and the North Shore confining unit—where the Lloyd aquifer, the Raritan confining unit, and the Magothy aquifer have been completely removed by glacial erosion.Water levels at 95 observation wells were measured quarterly during 1995–98. These data and continuous water-level records indicated that (1) the upper glacial (water-table) and Magothy aquifers are hydraulically connected and that their water levels did not respond to tidal fluctuations, and (2) the Lloyd and North Shore aquifers are hydraulically connected and their water levels responded to pumping and to tidal fluctuations.Marine seismic-reflection surveys in the surrounding embayments indicate at least four glacially eroded buried valleys with subhorizontal, parallel reflectors indicative of draped bedding that is interpreted as infilling by silt and clay. The buried valleys (1) truncate the surrounding coarse-grained deposits, (2) are asymmetrical and steep sided, (3) trend northwest-southeast, (4) are several miles long and about 1 mile wide, and (5) extend to more than 500 feet below sea level.Water samples taken during 1995–98 from three production wells and six observation wells screened in the upper glacial and Magothy aquifers contained volatile organic compounds in concentrations

  8. 76 FR 31679 - Saratoga and North Creek Railway, LLC-Operation Exemption-Warren County, NY

    Science.gov (United States)

    2011-06-01

    ... that it is negotiating the terms of an agreement with the County to restore common carrier rail freight... operate approximately 39.07 miles of rail line owned by Warren County, NY (the County), a noncarrier... Class III rail carrier and a subsidiary of Permian Basin Railways, Inc., which in turn is owned by Iowa...

  9. LiDAR Elevation Data Collection - Putnam County, NY, 2008 (NYSDEC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Summary of the surface elevation data collection project in Putnam County, NY (NYSDEC) 2008. Products generated include LiDAR point data in LAS Binary format v1.1....

  10. 75 FR 10547 - CSX Transportation, Inc.-Abandonment Exemption-in Jefferson County, NY

    Science.gov (United States)

    2010-03-08

    ... DEPARTMENT OF TRANSPORTATION Surface Transportation Board [STB Docket No. AB-55 (Sub-No. 695X)] CSX Transportation, Inc.--Abandonment Exemption--in Jefferson County, NY CSX Transportation, Inc. (CSXT) has filed a verified notice of exemption under 49 CFR 1152 Subpart F--Exempt Abandonments to abandon a 0.24-mile rail line, known as the Roe Feed...

  11. 76 FR 70110 - Foreign-Trade Zone 109-County of Jefferson, NY; Application for Reorganization and Expansion...

    Science.gov (United States)

    2011-11-10

    ... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 70-2011] Foreign-Trade Zone 109--County of Jefferson, NY; Application for Reorganization and Expansion Under Alternative Site Framework An application has been submitted to the Foreign-Trade Zones (FTZ) Board (the Board) by the County of Jefferson, grantee of FTZ 109, requesting authority...

  12. Simulation of Variable-Density Ground-Water Flow and Saltwater Intrusion beneath Manhasset Neck, Nassau County, New York, 1905-2005

    Science.gov (United States)

    Monti, Jack; Misut, Paul E.; Busciolano, Ronald J.

    2009-01-01

    The coastal-aquifer system of Manhasset Neck, Nassau County, New York, has been stressed by pumping, which has led to saltwater intrusion and the abandonment of one public-supply well in 1944. Measurements of chloride concentrations and water levels in 2004 from the deep, confined aquifers indicate active saltwater intrusion in response to public-supply pumping. A numerical model capable of simulating three-dimensional variable-density ground-water flow and solute transport in heterogeneous, anisotropic aquifers was developed using the U.S. Geological Survey finite-element, variable-density, solute-transport simulator SUTRA, to investigate the extent of saltwater intrusion beneath Manhasset Neck. The model is composed of eight layers representing the hydrogeologic system beneath Manhasset Neck. Four modifications to the area?s previously described hydrogeologic framework were made in the model (1) the bedrock-surface altitude at well N12191 was corrected from a previously reported value, (2) part of the extent of the Raritan confining unit was shifted, (3) part of the extent of the North Shore confining unit was shifted, and (4) a clay layer in the upper glacial aquifer was added in the central and southern parts of the Manhasset Neck peninsula. Ground-water flow and the location of the freshwater-saltwater interface were simulated for three conditions (time periods) (1) a steady-state (predevelopment) simulation of no pumping prior to about 1905, (2) a 40-year transient simulation based on 1939 pumpage representing the 1905-1944 period of gradual saltwater intrusion, and (3) a 60-year transient simulation based on 1995 pumpage representing the 1945-2005 period of stabilized withdrawals. The 1939 pumpage rate (12.1 million gallons per day (Mgal/d)) applied to the 1905-1944 transient simulation caused modeled average water-level declines of 2 and 4 feet (ft) in the shallow and deep aquifer systems from predevelopment conditions, respectively, a net decrease of 5

  13. 33 CFR 117.309 - Nassau Sound.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Nassau Sound. 117.309 Section 117.309 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.309 Nassau Sound. The draw of the Fernandina Port...

  14. Effects of decreased ground-water withdrawal on ground-water levels and chloride concentrations in Camden County, Georgia, and ground-water levels in Nassau County, Florida, from September 2001 to May 2003

    Science.gov (United States)

    Peck, Michael F.; McFadden, Keith W.; Leeth, David C.

    2005-01-01

    During October 2002, the Durango Paper Company formerly Gillman Paper Company) in St. Marys, Georgia, shut down paper-mill operations; the shutdown resulted in decreased ground-water withdrawal in Camden County by 35.6 million gallons per day. The decrease in withdrawal resulted in water-level rise in wells completed in the Floridan aquifer system and the overlying surficial and Brunswick aquifer systems; many wells in the St. Marys area flowed for the first time since the mill began operations during 1941. Pumping at the mill resulted in the development of a cone of depression that coalesced with a larger adjacent cone of depression at Fernandina Beach, Florida. Since closure of the mill, the cone at St. Marys is no longer present, although the cone still exists at Fernandina Beach, Florida. Historical water-level data from the production wells at the mill indicate that the pumping water level ranged from 68 to 235 feet (ft) below North American Vertical Datum of 1988 (NAVD 88) and averaged about 114 ft when the mill was operating. Since the shutdown, it is estimated that water levels at the mill have risen about 140 ft and are now at about 30 ft above NAVD 88. The water-level rise in wells in outlying areas in Camden County was less pronounced and ranged from about 5 to 10 ft above NAVD 88. Because of the regional upward water-level trend in the Upper Floridan aquifer that started during 19992000 in most of the coastal area, combined with a steeper upward trend beginning during October 2002, it was not possible to determine if the 510 ft rise in water levels in wells away from St. Marys was due to the mill closure. In addition to water-level rise of 2226 ft in the Floridan aquifer system, water-level rises in the overlying surficial and Brunswick aquifer systems at St. Marys after the shutdown indicate upward leakage of water. Water levels had stabilized in the confined surficial and Upper and Lower Floridan aquifers by AprilMay 2003; however, the water level in

  15. Using Major Elements to Determine Sources of Nitrate in Groundwater, Suffolk County, Long Island, NY

    Science.gov (United States)

    Munster, J.; Hanson, G.; Bokuniewicz, H.

    2004-05-01

    Suffolk County is the eastern most county on Long Island with an area of 2,500 square kilometers and a population of 1.4 million. Groundwater is the only source of potable water for Suffolk County. Nitrate levels have become a concern as a result of the continued eastward urbanization of Long Island since the mid 1900's. In 2003, 2% of 1000 public supply wells had greater than 10 ppm nitrogen as nitrate, 8% had 6 to 10 ppm nitrogen as nitrate and 62% of the wells were rated as susceptible to increased nitrate contamination based on land use, travel time and prevalence. Nitrogen as nitrate above 10 ppm is harmful to infants and is currently the drinking water standard of the Environmental Protection Agency. The major sources of the nitrate in the urbanized areas are most likely turf grass fertilizer and sewage from septic tank/cesspool systems and sewage treatment plants that provide only secondary treatment. Turf grass occupies about 28% of the land. Two-thirds of the houses have septic tank/cesspool systems and a majority of the sewage treatment plants discharge effluent to the groundwater. Previous investigators of the sources of nitrate in groundwater on Long Island have used 15N values of nitrate-nitrogen to identify nitrate contamination (Bleifuss et al., 2000; Flipse and Bonner, 1985; Flipse et al., 1984; Kreitler et al., 1978). However, due to overlapping source signatures, nitrogen isotopes alone were not sufficient to characterize the sources of nitrate. More recent studies have shown that major elements that accompany nitrate in the groundwater (Bleifuss et al., 2000; Elhatip et al., 2003; Trauth and Xanthopoulos, 1997) may distinguish sources of nitrate with less ambiguity. In this study samples of waste water from septic tank/cesspool systems and sewage treatment plants and samples of soil water collected below turf grass that is not fertilized, fertilized with organic fertilizer and fertilized with chemical fertilizer were analyzed for major elements

  16. National Dam Safety Program. Greenhaven Correction Facility Dam (Inventory Number N.Y. 11700), Lower Hudson River Basin, Duchess County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-09-09

    Facility am 11ational Damn Safety Prog~amDuhesCnt Visul IspecionLower Hudson River Basin. H ~ ydrology, Structural Stability TIh.ls repodrt.pravides... information and a ,.alvySi on tF’ hi. "~o of tae * dali as of the report date, Infornazl: nnd an~alysis are ba~z-td on visual inspectr~on of the dam by the...DUTCHESS COUNTY, N.Y. I. CONTENTS Page No. - ASSESSMENT S- OVERVIEW PHOTOGRAPH 1. PROJECT INFORMATION 1 1.1 GENERAL 1 a. Authority 1 b. Purpose of

  17. FLOODPLAIN, JEFFERSON COUNTY, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  18. 77 FR 46377 - Foreign-Trade Zone 37-Orange County, NY, Notification of Proposed Production Activity, Takasago...

    Science.gov (United States)

    2012-08-03

    ... Activity, Takasago International Corporation (Fragrances), Harriman, NY Takasago International Corporation (Takasago) submitted a notification of proposed production activity for their facility located in Harriman...-0002, and in the ``Reading Room'' section of the Board's Web site, which is accessible via www.trade...

  19. DIGITAL FLOOD INSURANCE RATE MAP DATABASE, NASSAU COUNTY, NEW YORK

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

  20. Superfund Record of Decision (EPA Region 2): Ramapo Landfill Site, Rockland County, NY. (First remedial action), March 1992. Final report

    International Nuclear Information System (INIS)

    1992-01-01

    The 60-acre former landfill site is located on a 96-acre tract in the Town of Ramapo, Rockland County, New York, about 35 miles northwest of New York City. Utility corridors lie on three sides of the site, including high-voltage power transmission lines. The site is currently being used as a compaction and transfer facility by the Town of Ramapo. Trash and debris are weighed at a weigh station/guardhouse, compacted at a baler facility in the northeastern corner of the site, and transferred to the Al Turi Landfill in Goshen, New York. The ROD represents the entire remedial action for the site by controlling source of contamination and the generation of leachate, and treatment of contaminated ground water. The primary contaminants of concern affecting soil, ground water, and surface water are VOCs, including benzene; other organics; and metals, including arsenic, chromium, and lead. The selected remedial action for the site is included

  1. Forandringsledelse Ny ledelsesstruktur - ny ledelsesudfordring

    DEFF Research Database (Denmark)

    Stryhn, Helle Majbritt

    2014-01-01

    for at imødekomme visioner i ny ledelsesstruktur. Der er brug for en evolutionær form, der kan tangere det revolutionære, idet det kendte og stabile i form at uddannelsesspecifikke opgaver, bliver udfordret af forandringer, der kan opleves næsten revolutionære, da det tværfaglige perspektiv skal være tydeligt i...

  2. Whose Bay Street? Competing Narratives of Nassau's City Centre

    Directory of Open Access Journals (Sweden)

    Nona Patara Martin

    2009-05-01

    Full Text Available Bay Street has always been at the centre of commercial, cultural and political life in the Bahama Islands. It also acts as a gateway for millions of tourists who come to Nassau, the Bahamian capital, via cruise ships every year. Not surprisingly, Bahamians and non-Bahamians have widely divergent impressions of Bay Street. The need to accommodate the tourists who are critical to the Bahamian economy has meant that Bay Street, despite its deep social significance for Bahamians, has increasingly become a tourist space. With reference to the ‘sense of place’ and place attachment literature, this paper traces the transformation of Bay Street and attempts to tease out the most obvious tensions between the Bay Street that Bahamians experience and Bay Street as a port of call.

  3. National Dam Safety Program. Smith Mills Reservoir Dam (Inventory Number NY-786), Lake Erie Basin, Chautauqua County, New York. Phase 1 Inspection Report

    Science.gov (United States)

    1980-03-01

    REPORT NUMBER Inventory No. 786 ;: 0 7. AUTHOR(s) 0 . CONTRACT OR GRANT NUMBER(&) George Koch ~ S. PERFORMING ORGANIZATION NAME AND ADDRESS 10. PROGRAM...UT A U Q U A C O U N T Y , / j < . INVENTORY No. NY 786 ’ .A T IO N A L D A M S A F E T Y P R O G R A M LL NEW YORK DISTRICT CORPS OF ENGINEERi i AR8...within the vicinity of the dam is generally northwestward, due to its location near the Portage Escarpment. Glacial cover is generally thin, the

  4. National Dam Safety Program. Hillburn Reservoir Dam (Inventory Number NY 974), Passaic River Basin, Rockland County, New York. Phase I Inspection Report.

    Science.gov (United States)

    1981-06-30

    Ieupcoemar an Identify by block rnumber) Natna S fet eyyrora Hilburn ’Reservoir*Dam Vioal n s aetyiPgr- Rockland County Visul Ispecion saic River Basin...o the 1dan a3 bit the report dae Information and F~nlj is ara based CKI .pIr:ial .0 inspecti&i of the darn ’ii:’ t*,., performing o-:Zizaton...196A-934 PASSAIC RIVER BASIN ROCKLAND COUNTY, NEW YORK TABLE OF CONTENTS PAGE NO. ASSESSMENT OVERVIEW PHOTOGRAPH PROJECT INFORMATION 1 1.1 GENERAL 1

  5. Northeast Florida Regional Sediment Management: Implementation Strategies and Recommendations for Nassau County and Duval County, Florida

    Science.gov (United States)

    2016-03-01

    million cubic meters ERDC/CHL TR-16-3 ix Acronyms ADCP Acoustic Doppler Current Profiler AIWW Atlantic Intracoastal Waterway CAP Continuing...UNF collected additional surveys to wading depths, as well as wave and current data using an Acoustic Doppler Current Profiler ( ADCP ) located in 30

  6. National Dam Safety Program. Tomahawk Lake Dam (Inventory Number N.Y. 618), Lower Hudson River Basin, Orange County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-08-14

    report (preliminary) for Orange County, prepared by Soil Conservation Service, the majority of surface materials consist of "Albia- Eldery " association...accordance with the plans and specifications so approved shall forfeit to the people of this state a sum not to exceed five hundred dollars to be fixed

  7. National Dam Safety Program. Watervliet Upper Dam (Inventory Number N.Y. 1356), Lower Hudson River Basin, Albany County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-07-29

    Program Watervliet Upper Dam Visul ispecion o"Albany County Hy.1 Visual 7 TStrcturlnii-- Lower Hudson River Basii 2O~ AN~ ~ - ~ ~ - ~ fe. 𔃼~ -- t7...1407 LOWER HUDSON RIVER BASIN TABLE OF CONTENTS PAGE NO. - ASSESSMENT - OVERVIEW PHOTOGRAPH 1 PROJECT INFORMATION 1 1.1 GENERAL 1 1.2 DESCRIPTION OF...inspection and maintenance of the dam and appurtenances. Document this information for future reference. 6. An emergency action plan must be developed. I

  8. National Dam Safety Program. Eaton Brook Reservoir Dam (Inventory Number NY 352), Susquehanna River Basin, Madison County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1980-02-19

    Visul IspecionWest Eaton pydrology, Structural Stability OeS iJiiTiAcr~U roawo p*Va *irow % ff n*,c...y sod Idenfi fr by block numbe.r) C) Tis report...provides inforMation andl analys-s on the physical condition of C-") I the dlam as of the report dnte. Information ’and analysis nre based~ on visual...SUSQUEHANNA RIVER BASIN MADISON COUNTY, NEW YORK TABLE OF CONTENTS PAGE NO. ASSESSMENT - OVERVIEW PHOTOGRAPH 1 PROJECT INFORMATION 1 1.1 GENERAL 1 1.2

  9. Patterns of color phase indicate spawn timing at a Nassau grouper Epinephelus striatus spawning aggregation

    Directory of Open Access Journals (Sweden)

    Stephanie K ARCHER, Scott A HEPPELL, Brice X SEMMENS,Christy V PATTENGILL-SEMMENS, Phillippe G BUSH, Croy M MCCOY,Bradley C JOHNSON

    2012-02-01

    Full Text Available Nassau grouper Epinephelus striatus are a large bodied, top level predator that is ecologically important throughout the Caribbean. Although typically solitary, Nassau grouper form large annual spawning aggregations at predictable times in specific locations. In 2003, The Cayman Islands Marine Conservation Board established protection for a newly rediscovered Nassau grouper spawning aggregation on Little Cayman, British West Indies. The large size of this aggregation provides a unique opportunity to study the behavior of Nassau grouper on a relatively intact spawning aggregation. During non-spawning periods Nassau grouper display a reddish-brown-and-white barred coloration. However, while aggregating they exhibit three additional color phases: “bicolor”, “dark”, and “white belly”. We video sampled the population on multiple days leading up to spawning across five spawning years. Divers focused a laser caliper equipped video camera on individual fish at the aggregation. We later analyzed the video to determine the length of the fish and record the color phase. Our observations show that the relative proportion of fish in the bicolor color phase increases significantly on the day leading up to the primary night of spawning. The increase in the proportion of the bicolor color phase from 0.05 early in the aggregation to 0.40 on the day of spawning suggests that this color phase conveys that a fish is behaviorally and physiologically prepared to spawn. Additionally, 82.7% of fish exhibiting dark or white belly coloration early in the aggregation period suggests that these color phases are not only shown by female fish as was previously posited [Current Zoology 58 (1: 73–83, 2012].

  10. Historical Processes and Contemporary Anthropogenic Activities Influence Genetic Population Dynamics of Nassau Grouper (Epinephelus striatus within The Bahamas

    Directory of Open Access Journals (Sweden)

    Krista D. Sherman

    2017-12-01

    Full Text Available Severe declines of endangered Nassau grouper (Epinephelus striatus across The Bahamas and Caribbean have spurred efforts to improve their fisheries management and population conservation. The Bahamas is reported to hold the majority of fish spawning aggregations for Nassau grouper, however, the status and genetic population structure of fish within the country is largely unknown, presenting a major knowledge gap for their sustainable management. Between August 2014–February 2017, 464 individual Nassau grouper sampled from The Bahamas were genotyped using 15 polymorphic microsatellite loci to establish measures of population structure, genetic diversity and effective population size (Ne. Nassau grouper were characterized by mostly high levels of genetic diversity, but we found no evidence for geographic population structure. Microsatellite analyses revealed weak, but significant genetic differentiation of Nassau grouper throughout the Bahamian archipelago (Global FST 0.00236, p = 0.0001. Temporal analyses of changes in Ne over the last 1,000 generations provide evidence in support of a pronounced historic decline in Bahamian Nassau grouper that appears to pre-date anthropogenic fishing activities. M-ratio results corroborate significant reductions in Ne throughout The Bahamas, with evidence for population bottlenecks in three islands and an active fish spawning aggregation along with apparent signs of inbreeding at two islands. Current estimates of Ne for Nassau grouper are considerably lower compared with historic levels. These findings represent important new contributions to our understanding of the evolutionary history, demographics and genetic connectivity of this endangered species, which are of critical importance for advancing their sustainable management.

  11. DCS Survey Submission for Cortland County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Survey data includes spatial datasets and data tables necessary to digitally represent data collected in the survey phase of the study. (Source: FEMA Guidelines and...

  12. DCS Hydraulics Submission for Albany County, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  13. DCS Hydraulic Submission for Ulster County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Recent developments in digital terrain and geospatial database management technology make it possible to protect this investment for existing and future projects to...

  14. DCS Hydrology Submission for Dutchess County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic processes for estimating flood discharges for a flood insurance...

  15. DCS Terrain Submission for Rockland County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describe the digital topographic data that were used to create...

  16. DCS Hydrology Submission for Putnam County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic processes for estimating flood discharges for a flood insurance...

  17. 2005 FEMA BE Lidar: Ulster County (NY)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describe the digital topographic data that were used to create...

  18. DCS Terrain Submission for Ulster County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Terrain data, as defined in FEMA Guidelines and Specifications, Appendix N: Data Capture Standards, describe the digital topographic data that were used to create...

  19. DCS Hydrology Submission for Rockland County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic procedures for estimating flood discharges for a flood insurance...

  20. DCS Hydrology Submission for Albany County NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydrology data include spatial datasets and data tables necessary for documenting the hydrologic processes for estimating flood discharges for a flood insurance...

  1. Ny rapport advarer

    DEFF Research Database (Denmark)

    Jex, Catherine; Seidenkrantz, Marit-Solveig; Colgan, William

    2017-01-01

    Ifølge forskerne bag en ny mellemstatslig rapport vil den globale opvarmning få endnu flere og meget drastiske konsekvenser for det arktiske klima end hidtil antaget. Men klimaaftalen fra Paris kan afværge nogle af de største forandringer efter år 2050. Hvis den bliver implementeret øjeblikkeligt...

  2. 75 FR 52021 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-08-24

    ... University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. Notice... University College of Dentistry, New York, NY. The human remains were removed from Port Clarence, Nome County... the human remains was made by New York University College of Dentistry professional staff in...

  3. Hydroacoustics for the discovery and quantification of Nassau grouper ( Epinephelus striatus) spawning aggregations

    Science.gov (United States)

    Egerton, J. P.; Johnson, A. F.; Le Vay, L.; McCoy, C. M.; Semmens, B. X.; Heppell, S. A.; Turner, J. R.

    2017-06-01

    Fish spawning aggregations (FSAs) are vital life-history events that need to be monitored to determine the health of aggregating populations; this is especially true of the endangered Nassau grouper ( Epinephelus striatus). Hydroacoustics were used to locate Nassau grouper FSAs at sites on the west end of Little Cayman (LCW), and east ends of Grand Cayman (GCE) and Cayman Brac (CBE). Fish abundance and biomass at each FSA were estimated via echo integration and FSA extent. Acoustic mean fish abundance estimates (±SE) on the FSA at LCW (893 ± 459) did not differ significantly from concurrent SCUBA estimates (1150 ± 75). Mean fish densities (number 1000 m-3) were significantly higher at LCW (33.13 ± 5.62) than at the other sites (GCE: 7.01 ± 2.1, CBE: 4.61 ± 1.16). We investigate different acoustic post-processing options to obtain target strength (TS), and we examine the different TS to total length (TL) formulas available. The SCUBA surveys also provided measures of TL through the use of laser callipers allowing development of an in situ TS to TL formula for Nassau grouper at the LCW FSA. Application of this formula revealed mean fish TL was significantly higher at LCW (65.4 ± 0.7 cm) than GCE (60.7 ± 0.4 cm), but not CBE (61.1 ± 2.5 cm). Use of the empirical TS to TL formula resulted in underestimation of fish length in comparison with diver measurements, highlighting the benefits of secondary length data and deriving specific TS to TL formulas for each population. FSA location examined with reference to seasonal marine protected areas (Designated Grouper Spawning Areas) showed FSAs were partially outside these areas at GCE and very close to the boundary at CBE. As FSAs often occur at the limits of safe diving operations, hydroacoustic technology provides an alternative method to monitor and inform future management of aggregating fish species.

  4. Tendenser i ny tysk litteratur

    DEFF Research Database (Denmark)

    Povlsen, Steen Klitgård

    Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)......Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)...

  5. Ny dansk museologi

    DEFF Research Database (Denmark)

    Museerne rummer både oplevelse og kulturarv. Det erfarer mere end 10 millioner besøgende hvert år. Tidligere har det været vigtigt at se på, hvordan man skulle drive museerne – men det bliver stadig mere interessant at fokusere på hvorfor.Ny dansk museologi undersøger museet som idé og medie...... danske samlinger og udstillinger er således af både historisk, strukturel, æstetisk og kommunikationsteoretisk karakter. Bruno Ingemann er leder af Center for Visuel Kommunikation på Roskilde Universitetscenter, og Ane Hejlskov Larsen er leder af Center for Museologi ved Aarhus Universitet....

  6. NASSAU 2006

    CERN Document Server

    Alp, E. E; 4th Nassau Mössbauer Symposium

    2007-01-01

    Progress in various areas of applications of Mössbauer Effect is reported in this volume. Among the recent developments, applications of synchrotron radiation in geophysics and biophysics through the measurement of phonon density of states of proteins and enzymes, and laboratory based high- pressure experiments in elucidating the electronic nature of correlated electron systems in condensed matter physics are some examples. Furthermore, almost after five decades of its discovery, with two Mössbauer spectrometers working on the surface of the Mars provided the first glimpse of the nature of iron containing minerals. With additional contributions from applications to nano-science and studies of amorphous systems will make this volume of special interest to materials scientists, chemists, mineral and condensed matter physicists, and inorganic biochemists alike. The conference and its proceedings are dedicated to one of the pioneers of Mössbauer Spectroscopy, late Dr. Stanley Ruby, whose original ideas, insigh...

  7. Dynamic camouflage by Nassau groupers Epinephelus striatus on a Caribbean coral reef.

    Science.gov (United States)

    Watson, A C; Siemann, L A; Hanlon, R T

    2014-11-01

    This field study describes the camouflage pattern repertoire, associated behaviours and speed of pattern change of Nassau groupers Epinephelus striatus at Little Cayman Island, British West Indies. Three basic camouflaged body patterns were observed under natural conditions and characterized quantitatively. The mean speed of pattern change across the entire body was 4.44 s (range = 0.97-9.87 s); the fastest pattern change as well as contrast change within a fixed pattern occurred within 1 s. Aside from apparent defensive camouflage, E. striatus used camouflage offensively to approach crustacean or fish prey, and three successful predation events were recorded. Although animal camouflage is a widespread tactic, dynamic camouflage is relatively uncommon and has been studied rarely in marine teleosts under natural conditions. The rapid changes observed in E. striatus suggest direct neural control of some skin colouration elements, and comparative studies of functional morphology and behaviour of colour change in other coral-reef teleosts are likely to reveal new mechanisms and adaptations of dynamic colouration. © 2014 The Fisheries Society of the British Isles.

  8. Oil spill monitoring and forecasting on the Prestige-Nassau accident

    Energy Technology Data Exchange (ETDEWEB)

    Montero, P.; Blanco, J.; Cabanas, J.M.; Maneiro, J.; Pazos, Y.; Morono, A. [Unidade de Observacion Proxima CPAM, Vilaxoan, Pontevedra (Spain); Balseiro, C.F.; Carracedo, P.; Gomez, B.; Penabad, E.; Perez-Munuzuri, V. [MeteoGalicia CMA, Santiago de Compostela (Spain); Braunschweig, F.; Fernandes, R.; Leitao, P.C.; Neves, R. [MARETEC IST, Lisbon (Portugal)

    2003-07-01

    The Prestige-Nassau tanker ship spilled about 10,000 tons of oil off the coast of Spain on November 13, 2002 during a severe storm. On November 19, the ship split in half and sank 133 nautical miles from the Galician coast to a depth of 3,500 metres, spilling another 20,000 tons of oil. The Galician government set up an Office of Nearshore Surveillance and recruited people from the Galician Regional Meteorological Service and the Spanish Institute of Oceanography to monitor the slick and forecast its trajectory. The main spill arrived at Galicia on November 30, damaging most of the coast. A variety of models that combined surface wind drift and ocean currents were used to forecast the movement of the spill. These included the Mothy from MeteoFrance, and DERIVA from the Portuguese Hydrographic Institute. Two models were also developed by MeteoGalicia and MAETEC. The path followed by the oil spill was classified in three parts. The first spill of 10,000 tons took place from November 13 until the ship split in two. The second spill of around 20,000 tons of oil occurred when the ship sank on November 19. The last spill includes oil that continued to leak from the sunken tanker at a rate of 125 tons per day. The trajectory predictions were found to be in good agreement with aerial observations. 24 refs., 10 figs.

  9. Inspiration fra NY-times

    DEFF Research Database (Denmark)

    Ejersbo, Lisser Rye

    2015-01-01

    NY-times har en ugentlig klumme med gode råd. For nogle uger siden var ugens inspiration henvendt til lærere/undervisere og drejede sig om, hvordan man skaber taletid til alle uden at have favoritter og overse de mere stille elever.......NY-times har en ugentlig klumme med gode råd. For nogle uger siden var ugens inspiration henvendt til lærere/undervisere og drejede sig om, hvordan man skaber taletid til alle uden at have favoritter og overse de mere stille elever....

  10. Udlejers godkendelse af ny erhvervslejer

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    2009-01-01

    I artiklen er taget udgangspunkt i den »klassiske« afståelsessituation, hvor en lejer vil afhænde den i de lejede lokaler drevne virksomhed, og lejeren har afståelsesret til en af udlejer godkendt lejer, uden at kravene til den ny lejer er specificeret. På baggrund af Vestre Landsrets dom af 9/2 ...

  11. Institutional analysis of marine reserves and fisheries governance policy experiments : a case study of Nassau grouper conservation in the Turks and Caicos Islands

    NARCIS (Netherlands)

    Rudd, M.A.

    2003-01-01

    Keywords: Ecosystem-based fisheries management; marine reserves; marine protected areas; social capital; institutional analysis; Turks and Caicos Islands; Nassau grouper Many tropical fisheries around the world are in crisis because of the depletion of valuable reef species and the destruction of

  12. The ups and downs of coral reef fishes: the genetic characteristics of a formerly severely overfished but currently recovering Nassau grouper fish spawning aggregation

    Science.gov (United States)

    Bernard, A. M.; Feldheim, K. A.; Nemeth, R.; Kadison, E.; Blondeau, J.; Semmens, B. X.; Shivji, M. S.

    2016-03-01

    The Nassau grouper ( Epinephelus striatus) has sustained large declines across its distribution, including extirpation of many of its fish spawning aggregations (FSAs). Within US Virgin Islands (USVI) waters, Nassau grouper FSAs were overfished until their disappearance in the 1970s and 1980s. In the early 2000s, however, Nassau grouper were found gathering at Grammanik Bank, USVI, a mesophotic coral reef adjacent to one of the extinct aggregation sites, and regulatory protective measures were implemented to protect this fledgling FSA. The population genetic dynamics of this rapid FSA deterioration followed by protection-facilitated, incipient recovery are unknown. We addressed two objectives: (1) we explored which factors (i.e., local vs. external recruitment) might be key in shaping the USVI FSA recovery; and (2) we examined the consequences of severe past overfishing on this FSA's current genetic status. We genotyped individuals (15 microsatellites) from the USVI FSA comprising three successive spawning years (2008-2010), as well as individuals from a much larger, presumably less impacted, Nassau grouper FSA in the Cayman Islands, to assess their comparative population dynamics. No population structure was detected between the USVI and Cayman FSAs ( F ST = -0.0004); however, a temporally waning, genetic bottleneck signal was detected in the USVI FSA. Parentage analysis failed to identify any parent-offspring matches between USVI FSA adults and nearby juveniles, and relatedness analysis showed low levels of genetic relatedness among USVI FSA individuals. Genetic diversity across USVI FSA temporal collections was relatively high, and no marked differences were found between the USVI and Cayman FSAs. These collective results suggest that external recruitment is an important driver of the USVI FSA recovery. Furthermore, despite an apparent genetic bottleneck, the genetic diversity of USVI Nassau grouper has not been severely compromised. Our findings also provide a

  13. NOAA Office for Coastal Management Coastal Inundation Digital Elevation Model: Jacksonville (FL) WFO - Duval, Clay, and Nassau Counties

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This digital elevation model (DEM) is a part of a series of DEMs produced for the National Oceanic and Atmospheric Administration Office for Coastal Management's Sea...

  14. Smart Growth Self-Assessment for Rural Communities: Madison County

    Science.gov (United States)

    Report from a technical assistance project to help Madison County, NY, develop a tool to help rural local governments assess how well their policies are helping them achieve the type of development they want.

  15. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d) A...

  16. Urban metabolism in Syracuse, NY – introduction

    Science.gov (United States)

    David J. Nowak

    2016-01-01

    This special issue of Urban Ecosystems contains a series of papers related to assessing urban metabolism in Syracuse, NY. These papers were developed under the Urban Long-Term Research Area Exploratory Awards Program funded by the National Science Foundation. Objectives of this two-year project (2009–2011) in Syracuse, NY were to investigate: a)...

  17. Introduktion til ny dansk museologi

    DEFF Research Database (Denmark)

    Ingemann, Bruno

    2005-01-01

    Museerne rummer både oplevelse og kulturarv. Det erfarer mere end 10 millioner besøgende hvert år. Tidligere har det været vigtigt at se på, hvordan man skulle drive museerne – men det bliver stadig mere interessant at fokusere på hvorfor.Ny dansk museologi undersøger museet som idé og medie...... danske samlinger og udstillinger er således af både historisk, strukturel, æstetisk og kommunikationsteoretisk karakter.Bruno Ingemann er leder af Center for Visuel Kommunikation på Roskilde Universitetscenter, og Ane Hejlskov Larsen er leder af Center for Museologi ved Aarhus Universitet....

  18. Urine antibody against human cancer antigen NY-ESO-1

    OpenAIRE

    Jäger, Dirk; Stockert, Elisabeth; Karbach, Julia; Herrlinger, Kristina; Atmaca, Akin; Arand, Michael; Chen, Yao-Tseng; Gnjatic, Sacha; Old, Lloyd J; Knuth, Alexander; Jäger, Elke

    2002-01-01

    NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NY-ESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1...

  19. DIGITAL FLOOD INSURANCE RATE MAP DATABASE ROCKLAND COUNTY, NY, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Digital Flood Insurance Rate Map (DFIRM) Database depicts flood risk information and supporting data used to develop the risk data. The primary risk...

  20. 77 FR 1550 - Environmental Impact Statement: Erie County, NY

    Science.gov (United States)

    2012-01-10

    ... improvements and minor construction projects and will not be seeking Federal funding for such work. In light of... DEPARTMENT OF TRANSPORTATION Federal Highway Administration Environmental Impact Statement: Erie... that FHWA will not be preparing and issuing a Final Environmental Impact Statement for the proposed...

  1. Floodplain Mapping Submission for Village of Dansville, Livingston County, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  2. 75 FR 54419 - Environmental Impact Statement: Queens County, NY

    Science.gov (United States)

    2010-09-07

    ... published in the Federal Register on March 12, 2004. FOR FURTHER INFORMATION CONTACT: Jeffrey W. Kolb... NEPA Class II Categorical Exclusion. Issued on August 25, 2010. Jeffrey W. Kolb, Division Administrator...

  3. County Spending

    Data.gov (United States)

    Montgomery County of Maryland — This dataset includes County spending data for Montgomery County government. It does not include agency spending. Data considered sensitive or confidential and will...

  4. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Science.gov (United States)

    2010-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones Inlet...

  5. 2008 Federal Emergency Management Agency (FEMA) New York Lidar: Erie County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Using Leica Geosystems ALS50 Laser Systems, 89 flight lines with a nominal point spacing = 1.4 meters (4.59 feet) were collected over Erie County, NY (approximately...

  6. Multikulturalisme er en ny slags Apartheid

    DEFF Research Database (Denmark)

    Dyrberg, Torben Bech

    2016-01-01

    I en ny antologi retter forfatterne et kritisk lys mod forsvarerne af multikulturalisme. Og det er der god grund til, mener en af bogens redaktører. For den store gruppe, der hylder multikulturen, glemmer i al dens godhed, at de samtidig er med til at undergrave demokratiet og styrke uheldige mag...

  7. 76 FR 64375 - Iroquois National Wildlife Refuge, Genesee County and Orleans County, NY; Final Comprehensive...

    Science.gov (United States)

    2011-10-18

    ... 61171, October 4, 2010). Iroquois NWR was established in 1958 under the Migratory Bird Conservation Act for ``* * * use as an inviolate sanctuary, or for any other management purpose, for migratory birds... management, monitor waterfowl during spring and fall migration, conduct landbird surveys, and manage for...

  8. TIGER/Line Shapefile, 2013, county, Westchester County, NY, Current Address Ranges Relationship File

    Data.gov (United States)

    US Census Bureau, Department of Commerce — The TIGER/Line shapefiles and related database files (.dbf) are an extract of selected geographic and cartographic information from the U.S. Census Bureau's Master...

  9. Mot en ny pedagogisk lederrolle og lederidentitet?

    Directory of Open Access Journals (Sweden)

    Ann Kristin Larsen

    2014-02-01

    Full Text Available Artikkelen omhandler hvordan endrede ledelsesstrukturer oppstått gjennom ny organisasjonsmodell virker inn på de pedagogiske ledernes arbeidsoppgaver. Vi har etterspurt deres erfaringer knyttet til mulige endringer i oppgaver, arbeidsdeling og faglig-pedagogisk fokus i arbeidet. Undersøkelsen ble gjennomført i en bydel i Oslo. Hovedfunnene er at pedagogiske ledere har fått flere administrative oppgaver og personalleder oppgaver. De pedagogiske lederne deler seg i to med hensyn til hvor fornøyde de er med disse endringene. I artikkelen drøfter vi hva denne todelingen betyr for deres rolleforståelse og identitet.

  10. DCS TERRAIN SUBMISSION for ONEIDA COUNTY, NEW York, USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — For Oneida County, NY, there were two types of elevation datasets. The first type is LiDAR and the second one is Auto-correlation DEM. Auto-correlation DEM data was...

  11. 33 CFR 110.208 - Buffalo Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds—(1...

  12. NY-ESO-1 specific antibody and cellular responses in melanoma patients primed with NY-ESO-1 protein in ISCOMATRIX and boosted with recombinant NY-ESO-1 fowlpox virus.

    Science.gov (United States)

    Chen, Ji-Li; Dawoodji, Amina; Tarlton, Andrea; Gnjatic, Sacha; Tajar, Abdelouahid; Karydis, Ioannis; Browning, Judy; Pratap, Sarah; Verfaille, Christian; Venhaus, Ralph R; Pan, Linda; Altman, Douglas G; Cebon, Jonathan S; Old, Lloyd L; Nathan, Paul; Ottensmeier, Christian; Middleton, Mark; Cerundolo, Vincenzo

    2015-03-15

    Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8(+) or CD4(+) T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4(+) T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8(+) T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8(+) T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4+ T cells and NY-ESO-185-108 for CD8+ T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes. © 2014 UICC.

  13. Sjøforsvaret i ny drakt

    DEFF Research Database (Denmark)

    Vestvik, Marit; Bjerck, Mari

    Denne rapporten formidler resultater fra undersøkelser som er gjort på to av Sjøforsvarets fartøy i 2010 og 2011, som omfatter utprøving av ny niformsmodell for utendørs bruk. Undersøkelsene er en del av forskningsprosjektet Uni-Form: Arbeidsbekledning for kvinner i mannsdominerte yrker. I...... prosjektet utvikles arbeidsklær for Sjøforsvarets personell som har bedre funksjonalitet og komfort enn eksisterende uniformer. En målsetning er å bedre trivselen for personellet gjennom å utvikle forbedrete uniformbekledning. At uniformsbekledningen er tilfredsstillende i funksjonalitet, passform og...... tilpasset snitt for kvinner. Dette gjelder også Forsvarets uniformer....

  14. EnviroAtlas - New York, NY - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the New York, NY EnviroAtlas community. The block groups are from the US Census Bureau and are included/excluded based...

  15. RenewableNY - An Industrial Energy Conservation Initiative

    Energy Technology Data Exchange (ETDEWEB)

    Lubarr, Tzipora

    2009-09-30

    The New York Industrial Retention Network (NYIRN) manages the RenewableNY program to assist industrial companies in New York City to implement energy efficiency projects. RenewableNY provides companies with project management assistance and grants to identify opportunities for energy savings and implement energy efficiency projects. The program helps companies identify energy efficient projects, complete an energy audit, and connect with energy contractors who install renewable energy and energy efficient equipment. It also provides grants to help cover the costs of installation for new systems and equipment. RenewableNY demonstrates that a small grant program that also provides project management assistance can incentivize companies to implement energy efficiency projects that might otherwise be avoided. Estimated savings through RenewableNY include 324,500 kWh saved through efficiency installations, 158 kW of solar energy systems installed, and 945 thm of gas avoided.

  16. Indian scientific endeavors in Ny-Ålesund, Arctic

    Directory of Open Access Journals (Sweden)

    Neloy Khare

    2009-03-01

    Full Text Available An ice free Arctic can effect tropical climate and is therefore a key variable to understand the climate change. In order to undertake studies in the Arctic region, on 1st July 2008, the Indian station ‘Himadri’ has been opened in Ny-Ålesund. With India, 15 stations from 10 countries are now established in Ny-Ålesund. This report highlights Indian initiatives in the Arctic region.

  17. 33 CFR 110.84b - Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo, N.Y. 110.84b Section 110... REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as Port of Buffalo Small Boat Harbor commencing at a point on shore at latitude 42°51′05″ N., longitude 78°51...

  18. Autoimmune thyroid disease elicited by NY-ESO-1 vaccination.

    Science.gov (United States)

    Vita, Roberto; Guarneri, Fabrizio; Agah, Ravin; Benvenga, Salvatore

    2014-02-01

    Immunotherapies and targeted therapies are frequently associated with thyroid dysfunction, which is in contrast with the rare thyroid abnormalities induced by cytotoxic agents. Immunotherapy with NY-ESO-1, a tumor-associated antigen expressed by a number of malignancies, was reported to trigger hyperthyroidism or hypothyroidism in two HLA-A2 patients with ovarian cancer. We describe now a case of Graves' disease triggered by NY-ESO-1 in a HLA-A2-negative woman. A 32-year-old woman with a synovial sarcoma received radiotherapy, chemotherapy, and finally NY-ESO-1 vaccine. The patient was found to have HLA A11/A33(19), B13/B56(22), Cw3/-. One month after the beginning of immunotherapy, thyroid dysfunction was clinically suspected and Graves' disease was biochemically confirmed. Fearful of the antithyroid drugs' side effects, the patient was treated with a beta-blocker (propranolol, 80-20 mg/day). As hyperthyroidism progressively worsened, the patient underwent total thyroidectomy. We hypothesized that NY-ESO-1 shared partial homology with thyroid autoantigens (the so-called molecular mimicry mechanism) and that at least one pair of homologous sequences contained amino acid sequence binding motifs to a restricted number of HLA molecules. We used BLAST software to search amino acid sequence homologies between NY-ESO-1 and thyroid autoantigens (thyrotropin receptor [TSH-R], thyroperoxidase, and thyroglobulin), and the HLA ligand/motif database to look for HLA/T-cell receptor binding motifs in the regions of NY-ESO-1 and thyroid autoantigens that were homologous. We found 15 epitopic regions of NY-ESO-1 homologous to 15 regions of thyroid autoantigens, some of which epitopic: 5 of TSH-R, 8 of thyroglobulin, and 2 of thyroperoxidase. These homologous sequences contain binding motifs belonging to several HLA class I antigens, including HLA A2 and the patient's A11 and A33. Genetically predisposed patients who receive NY-ESO-1 vaccination are at risk to develop thyroid

  19. En ny generasjon troverdige kliniske retningslinjer

    Directory of Open Access Journals (Sweden)

    Per Olav Vandvik

    2013-11-01

    Full Text Available Trygg og effektiv diagnostikk, pleie og behandling fordrer at helsepersonell har tilgang til oppdatert og gyldig forskningsbasert kunnskap, fortrinnsvis i form av faglige kliniske retningslinjer utviklet med tilfredsstillende metodikk. De fleste retningslinjer har imidlertid betydelige svakheter med hensyn til både kunnskapsgrunnlag, utvikling av anbefalinger, presentasjonsformater og oppdatering. Nye definisjoner, standarder og metoder for kunnskapsbaserte troverdige retningslinjer gir langt bedre muligheter enn tidligere for å lykkes i arbeidet med å utvikle og implementere retningslinjer, men stiller samtidig større krav til metodekompetanse, klinisk ekspertise og tid. I vår digitale tidsalder åpner ny nettbasert teknologi for bedre disseminering av retningslinjer til frontlinjene i klinikken, og til å dele kunnskap med pasienter i konsultasjonen, for eksempel gjennom smarttelefoner og lesebrett. Gjennom et praktisk eksempel redegjør vi i denne artikkelen for nye definisjoner, standarder, metoder og verktøy for utvikling, disseminering og oppdatering av faglige retningslinjer. GRADE-systemet er en sentral komponent i dette. Det er nå mulig å lage troverdige retningslinjer som klinikere og pasienter kan ta i bruk i en travel hverdag. Det gjenstår å se hvordan myndighetene og fagmiljøene vil bidra for å sikre pasienter trygg og effektiv behandling gjennom utvikling av denne nye generasjonen av kliniske retningslinjer.Vandvik PO, Berg R, Vist G. A new generation of trustworthy clinical practice guidelines. Nor J Epidemiol 2013; 23 (2: 197-204.ENGLISH SUMMARYSafe and effective diagnosis and treatment requires that health care personnel can access the best current research evidence, preferably through clinical practice guidelines. Most guidelines suffer from methodological weaknesses (e.g., identification and assessment of research evidence, development of recommendations, suboptimal presentation formats and infrequent updating of

  20. 33 CFR 110.87 - Henderson Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Henderson Harbor, N.Y. 110.87... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in the... latitude 43°51′08.8″ N, longitude 76°12′08.9″ W, thence to latitude 43°51′09.0″ N, longitude 76°12′19.0″ W...

  1. Evaluering af ny kokkeuddannelse for hørehandicappede

    DEFF Research Database (Denmark)

    Hansen, Niels-Henrik Møller

    2010-01-01

    Center for Ungdomsforskning har netop afsluttet en evaluering af en ny uddannelse indenfor madproduktion, rettet imod personer med et hørehandicap. Hensigten med uddannelsen er, at kunne tilbyde døve, døvblinde og hørehæmmede personer et uddannelsestilbud indenfor fremstilling af mad. Konkret han...

  2. 77 FR 22530 - Safety Zone; Fireworks, Hudson River, Rhinecliff, NY

    Science.gov (United States)

    2012-04-16

    ... fireworks display for a wedding ceremony on the waters of the Hudson River in the vicinity of Rhinecliff, NY... health or risk to safety that might disproportionately affect children. Indian Tribal Governments This... with Indian Tribal Governments, because it would not have a substantial direct effect on one or more...

  3. Ny struktur - et udviklingsprojekt på Skanderborg Kulturskole

    DEFF Research Database (Denmark)

    Holst, Finn

    I 2010 tog Skanderborg Musikskole initiativ til et udviklingsprojekt med betegnelsen ”Ny Struktur i Skanderborg Musikskole”. Baggrunden herfor er i henhold til Musikskolens leder, at lærere, bestyrelse og ledelse har diskuteret, hvordan man kunne gøre noget ved nogle af de problemer eller svaghed...

  4. Ejendomsordbogen fransk/dansk: ny integreret e-ordbog

    DEFF Research Database (Denmark)

    Leroyer, Patrick; Hansen, Liselotte Kruse

    2012-01-01

    fusionere med en praktisk guide. Til dette formål har vi udviklet konceptet til en ny type hybridordbog, der kan tilfredsstille de intenderede brugeres informationsbehov i kommunikative, kognitive og praktiske situationer. Udviklingen er sket i samarbejde med den førende udbyder af online ordbøger i Danmark...

  5. Interoperability of Demand Response Resources Demonstration in NY

    Energy Technology Data Exchange (ETDEWEB)

    Wellington, Andre

    2014-03-31

    The Interoperability of Demand Response Resources Demonstration in NY (Interoperability Project) was awarded to Con Edison in 2009. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited Demand Response resources to integrate more effectively with electric delivery companies and regional transmission organizations.

  6. Ny international efteruddannelse for fødevaresektoren

    DEFF Research Database (Denmark)

    Stacey, Julia; Brunsø, Karen

    2003-01-01

    Ny MBA på Handelshøjskolen i Århus stiller skarpt på fødevaresektoren. MBA - med fokus på fødevaresektoren henvender sig til personer med minimum tre års ledererfaring, der ønsker nye erhvervsrettede kompetencer på højt niveau....

  7. Rapid variability of the EXor star NY Ori

    Science.gov (United States)

    Lorenzetti, D.; Arkharov, A. A.; Efimova, N.; Giannini, T.; Antoniucci, S.; Di Paola, A.; Larionov, V. M.

    2016-03-01

    We report on a rapid brightness variability of the classical EXor star NY Ori observed with the AZT24 1m IR telescope (Campo Imperatore, Italy), as a part of our program EXORCISM (EXOR OptiCal and Infrared Systematic Monitoring - Antoniucci et al. 2013 PPVI; Lorenzetti et al. 2009 ApJ 693, 1056).

  8. Kapitalismens dna i ny oversættelse

    DEFF Research Database (Denmark)

    Schanz, Hans-Jørgen

    2013-01-01

    Adam Smiths klassiske storværk om kapitalismen ’Nationernes velstand’ havde stor indflydelse på socialfilosofien, på Hegel, på Marx og liberaløkonomerne. Ny oversættelse yder omsider værket fuld respekt på dansk. Anmeldelse af: Adam Smith: Nationernes velstand. 441+ 519 sider. 699,95 kr...

  9. NY-ESO-1 cancer testis antigen demonstrates high immunogenicity in triple negative breast cancer.

    Science.gov (United States)

    Ademuyiwa, Foluso O; Bshara, Wiam; Attwood, Kristopher; Morrison, Carl; Edge, Stephen B; Karpf, Adam R; James, Smith A; Ambrosone, Christine B; O'Connor, Tracey L; Levine, Ellis G; Miliotto, Anthony; Ritter, Erika; Ritter, Gerd; Gnjatic, Sacha; Odunsi, Kunle

    2012-01-01

    NY-ESO-1 cancer testis (CT) antigen is an attractive candidate for immunotherapy as a result of its high immunogenicity. The aim of this study was to explore the potential for NY-ESO-1 antigen directed immunotherapy in triple negative breast cancer (TNBC) by determining the frequency of expression by immunohistochemistry (IHC) and the degree of inherent immunogenicity to NY-ESO-1. 168 TNBC and 47 ER+/HER2- primary breast cancer specimens were used to determine NY-ESO-1 frequency by IHC. As previous studies have shown that patients with a robust innate humoral immune response to CT antigens are more likely to develop CD8 T-cell responses to NY-ESO-1 peptides, we evaluated the degree to which patients with NY-ESO-1 expression had inherent immunogenicity by measuring antibodies. The relationship between NY-ESO-1 expression and CD8+ T lymphocytes was also examined. The frequency of NY-ESO-1 expression in the TNBC cohort was 16% versus 2% in ER+/HER2- patients. A higher NY-ESO-1 score was associated with a younger age at diagnosis in the TNBC patients with NY-ESO-1 expression (p = 0.026). No differences in OS (p = 0.278) or PFS (p = 0.238) by NY-ESO-1 expression status were detected. Antibody responses to NY-ESO-1 were found in 73% of TNBC patients whose tumors were NY-ESO-1 positive. NY-ESO-1 positive patients had higher CD8 counts than negative patients (p = 0.018). NY-ESO-1 is expressed in a substantial subset of TNBC patients and leads to a high humoral immune response in a large proportion of these individuals. Given these observations, patients with TNBC may benefit from targeted therapies directed against NY-ESO-1.

  10. 33 CFR 110.8 - Lake Champlain, N.Y. and Vt.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Lake Champlain, N.Y. and Vt. 110... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.8 Lake Champlain, N.Y. and Vt. (a) Ticonderoga, N.Y. An area shoreward of a line bearing 312° from Ticonderoga Light to the southeast corner of the...

  11. 78 FR 1713 - Golden Nematode; Removal of Regulated Areas in Livingston and Steuben Counties, NY

    Science.gov (United States)

    2013-01-09

    ... wide hedgerow to coordinates 42 42'83.1'', - 72'00.3''; then south through woods, along a farm road...'', - 53'72.2''; then west along a forest edge and a hedgerow to the east side of Rex Road to coordinates...'', - 54'53.6''; then south along a hedgerow and a forest edge to coordinates 42 45'05.7'', - 54'54.7...

  12. 77 FR 30312 - Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY; Draft Comprehensive...

    Science.gov (United States)

    2012-05-22

    .... The refuge's public use program provides wildlife-oriented educational and recreational opportunities... visitor center, observation towers and platforms, fishing access sites, hunting programs, trapping program, educational programs and materials, guided tours, and other special programs. [[Page 30313

  13. National Dam Safety Program. Court Street Dam (Inventory Number NY 6843), Monroe County, New York

    Science.gov (United States)

    1980-09-30

    i.aoa 271.aO 31U7c .tI1S32cC. CS3Jit 143421e. li1i3 i. Im u.. Cu 2 12w6,. tD ?3Ut. liczvl,. -23ci11. a~ij1 r 2 nitlGArSA Li 1% cf 1,s37. z12 1n39 0322 i...1839ts. 27897u. CA S 1iu7. 1404. 1090. ill . 190u. 1:,CHES 1.o0 5.59 sou2 10.14 A 445.77 142.U5 203.7b 2b7 .s8 AC-Ff 2,*589. 7osid. 109474. 130i32...D7i. V-625- 2644. 70243. 9460. _ -59t -S,--2 5 _ _172.- - C 0373.. J 4U 2b7 4D7io. . 118. lu 08.6 1!)4.,5 i22.i7 Ae b!)385. i4t0o1l. D39i1 4. doob

  14. Floodplain Mapping Submission for Town of North Dansville, Livingston County, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  15. 75 FR 25286 - Montezuma National Wildlife Refuge, Seneca, Wayne, and Cayuga Counties, NY

    Science.gov (United States)

    2010-05-07

    ... to the area. Scoping: Preliminary Issues, Concerns, and Opportunities The planning team is in the... may be raised. (1) Ecoregional or ecosystemwide issues, such as climate change, regional land..., inventories, and research; (3) Public-use program issues, such as the breadth and quality of programs, public...

  16. 78 FR 19000 - Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY; Final Comprehensive...

    Science.gov (United States)

    2013-03-28

    ... acquired lands for their potential for habitat restoration (i.e., emergent marsh, forest, shrubland... emergent marsh habitats, once lands have been acquired and restored. We also revised the land protection... habitats include emergent marshes and shallow water mudflats, open water, bottomland floodplain forest, old...

  17. 78 FR 3827 - Golden Nematode; Removal of Regulated Areas in Livingston and Steuben Counties, NY

    Science.gov (United States)

    2013-01-17

    ...) That portion of land in the town of Avon bounded as follows: Beginning at a point marked by latitude... follows: Beginning at a point along the west bank of the Marsh Ditch that intersects a farm road marked by latitude/ longitude coordinates 42[deg]42'30'', -77[deg]71'21''; then north along the Marsh Ditch to...

  18. 76 FR 67672 - Proposed Foreign-Trade Zone; Genesee County, NY, Under Alternative Site Framework

    Science.gov (United States)

    2011-11-02

    ... Framework An application has been submitted to the Foreign-Trade Zones (FTZ) Board (the Board) by the... entry, under the alternative site framework (ASF) adopted by the Board (74 FR 1170-1173, 1/12/09...

  19. Den fjerde vej: Trumps sejr skaber ny samfundsorden

    DEFF Research Database (Denmark)

    Rasmussen, Martin Møller Boje; Andersen, Torben K.; Kragh, Claus

    2016-01-01

    Bag Donald Trumps uempatiske retorik gemmer sig omridset af et politisk projekt, man kan kalde den fjerde vej. USA’s kommende præsident blander højre- og venstreorienterede mærkesager til et nyt nationalsocialt politisk koncept. Spørgsmålet er, om Trumps fjerde vej er et seriøst bud på en ny...

  20. Ny forskning: Derfor virker ros ikke mod stress

    DEFF Research Database (Denmark)

    Pedersen, Pernille

    2015-01-01

    Ny forskning peger på, at stressede medarbejdere overhører din ros og anerkendelse, hvis de føler skam. Skam over ikke at kunne slå til på arbejdet. Vil du hjælpe en stresset medarbejder, skal du forebygge, at de føler sig skamfulde. Læs her anbefalinger til, hvordan du bedst hjælper din stressed...

  1. Allegheny County Municipal Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates the municipal boundaries in Allegheny County. Data was created to portray the boundaries of the 130 Municipalities in Allegheny County the...

  2. Allegheny County Addressing Landmarks

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains address points which represent physical address locations assigned by the Allegheny County addressing authority. Data is updated by County...

  3. Allegheny County Council Districts

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset portrays the boundaries of the County Council Districts in Allegheny County. The dataset is based on municipal boundaries and City of Pittsburgh ward...

  4. Allegheny County Address Points

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains address points which represent physical address locations assigned by the Allegheny County addressing authority. Data is updated by County...

  5. Allegheny County Air Quality

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Air quality data from Allegheny County Health Department monitors throughout the county. Air quality monitored data must be verified by qualified individuals before...

  6. NY-ESO-1 antibody as a novel tumour marker of gastric cancer.

    Science.gov (United States)

    Fujiwara, S; Wada, H; Kawada, J; Kawabata, R; Takahashi, T; Fujita, J; Hirao, T; Shibata, K; Makari, Y; Iijima, S; Nishikawa, H; Jungbluth, A A; Nakamura, Y; Kurokawa, Y; Yamasaki, M; Miyata, H; Nakajima, K; Takiguchi, S; Nakayama, E; Mori, M; Doki, Y

    2013-03-19

    NY-ESO-1 antibodies are specifically observed in patients with NY-ESO-1-expressing tumours. We analysed whether the NY-ESO-1 humoral immune response is a useful tumour marker of gastric cancer. Sera from 363 gastric cancer patients were screened by enzyme-linked immunosorbent assay (ELISA) to detect NY-ESO-1 antibodies. Serial serum samples were obtained from 25 NY-ESO-1 antibody-positive patients, including 16 patients with curative resection and 9 patients who received chemotherapy alone. NY-ESO-1 antibodies were detected in 3.4% of stage I, 4.4% of stage II, 25.3% of stage III, and 20.0% of stage IV patients. The frequency of antibody positivity increased with disease progression. When the NY-ESO-1 antibody was used in combination with carcinoembryonic antigen and CA19-9 to detect gastric cancer, information gains of 11.2% in stages III and IV, and 5.8% in all patients were observed. The NY-ESO-1 immune response levels of the patients without recurrence fell below the cutoff level after surgery. Two of the patients with recurrence displayed incomplete decreases. The nine patients who received chemotherapy alone continued to display NY-ESO-1 immune responses. When combined with conventional tumour markers, the NY-ESO-1 humoral immune response could be a useful tumour marker for detecting advanced gastric cancer and inferring the post-treatment tumour load in seropositive patients.

  7. NY-ESO-1 expression and immunogenicity in prostate cancer patients.

    Science.gov (United States)

    Gati, Asma; Lajmi, Nesrine; Derouiche, Amine; Marrakchi, Raja; Chebil, Mohamed; Benammar-Elgaaied, Amel

    2011-10-01

    Prostate cancer is the second leading cause of men cancer-related death. Cancer immunotherapy has been investigated as a treatment which might be instituted at the point of detection of androgen-independent metastatic disease. to investigate the expression and humoral response against NYESO-1 in patients with prostate cancer (PC) and to analyze the relationship between expression of NY-ESO-1 and clinicopathological features. NY-ESO-1 mRNA in surgically resected PC and benign prostatic hyperplasia (BPH) were examined by reverse transcriptionpolymerase chain reaction. The antibody response to NY-ESO-1 was examined by enzyme-linked Elisa assay using recombinant NYESO-1 protein. NY-ESO-1 mRNA was detected in 9 of 23 (39%) PC patients. Antibodies against NY-ESO-1 protein were detected in 12 of 23 (52%) sera of PC patients and in 5 of 9 (55%) of NY-ESO-1 expressing tumors. However, no mRNA copy or NY-ESO-1 antibodies were detected in all BPH patients tested. The present study has demonstrated the expression of NY-ESO-1mRNA in prostate Cancer patients and NY-ESO-1 antibody production. Our data suggest that NY-ESO-1 could be used as a tumor marker and constitute a good candidate for vaccine-based immunotherapy for hormonal resistant prostate cancer patients.

  8. Vaccination with NY-ESO-1 overlapping peptides mixed with Picibanil OK-432 and montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Wada, Hisashi; Isobe, Midori; Kakimi, Kazuhiro; Mizote, Yu; Eikawa, Shingo; Sato, Eiichi; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Yamasaki, Makoto; Miyata, Hiroshi; Matsushita, Hirokazu; Udono, Heiichiro; Seto, Yasuyuki; Yamada, Kazuhiro; Nishikawa, Hiroyoshi; Pan, Linda; Venhaus, Ralph; Oka, Mikio; Doki, Yuichiro; Nakayama, Eiichi

    2014-01-01

    We conducted a clinical trial of an NY-ESO-1 cancer vaccine using 4 synthetic overlapping long peptides (OLP; peptides #1, 79-108; #2, 100-129; #3, 121-150; and #4, 142-173) that include a highly immunogenic region of the NY-ESO-1 molecule. Nine patients were immunized with 0.25 mg each of three 30-mer and a 32-mer long NY-ESO-1 OLP mixed with 0.2 KE Picibanil OK-432 and 1.25 mL Montanide ISA-51. The primary endpoints of this study were safety and NY-ESO-1 immune responses. Five to 18 injections of the NY-ESO-1 OLP vaccine were well tolerated. Vaccine-related adverse events observed were fever and injection site reaction (grade 1 and 2). Two patients showed stable disease after vaccination. An NY-ESO-1-specific humoral immune response was observed in all patients and an antibody against peptide #3 (121-150) was detected firstly and strongly after vaccination. NY-ESO-1 CD4 and CD8 T-cell responses were elicited in these patients and their epitopes were identified. Using a multifunctional cytokine assay, the number of single or double cytokine-producing cells was increased in NY-ESO-1-specific CD4 and CD8 T cells after vaccination. Multiple cytokine-producing cells were observed in PD-1 (-) and PD-1 (+) CD4 T cells. In conclusion, our study indicated that the NY-ESO-1 OLP vaccine mixed with Picibanil OK-432 and Montanide ISA-51 was well tolerated and elicited NY-ESO-1-specific humoral and CD4 and CD8 T-cell responses in immunized patients.

  9. NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses.

    Science.gov (United States)

    Wadle, Andreas; Mischo, Axel; Strahl, Sabine; Nishikawa, Hiroyoshi; Held, Gerhard; Neumann, Frank; Wullner, Beate; Fischer, Eliane; Kleber, Sascha; Karbach, Julia; Jager, Elke; Shiku, Hiroshi; Odunsi, Kunle; Shrikant, Protul A; Knuth, Alexander; Cerundolo, Vincenzo; Renner, Christoph

    2010-11-01

    Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4(+) T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development. Copyright © 2010 John Wiley & Sons, Ltd.

  10. Anti-NY-ESO-1 autoantibody may be a tumor marker for intrahepatic cholangiocarcinoma.

    Science.gov (United States)

    Zhang, Zhen; Li, Fan-Fan; Lu, Ming-Dian; Zhang, Shang-Xin; Li, Yong-Xiang

    2017-11-28

    Anti-NY-ESO-1 antibody is observed in a multitude of malignancies. This study was aimed to evaluate the expression of serum anti-NY-ESO-1 antibodies and its prognostic value in intrahepatic cholangiocarcinoma. A total of 103 patients with intrahepatic cholangiocarcinoma were enrolled in the study. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum level of anti-NY-ESO-1 antibody. Western blotting was performed to assess the NY-ESO-1 expression in tumor and adjacent tissues. The serum NY-ESO-1 antibody was detected in 18.4% of patients with intrahepatic cholangiocarcinoma, a value that was significantly higher than that in patients with chronic Hepatitis B. Serum NY-ESO-1 antibody was positively correlated with tumor differentiation, lymphatic metastasis, cTNM stage and abdominal pain. Finally, there was a higher cumulative survival rate in patients with serum NY-ESO-1 positivity than in those with serum NY-ESO-1 negativity among the patients with stage III + IV. Our data uncovered that NY-ESO-1 antibody might be a helpful tumor marker and prognostic predictor in intrahepatic cholangiocarcinoma.

  11. NY-ESO-1 expression in synovial sarcoma and other mesenchymal tumors: significance for NY-ESO-1-based targeted therapy and differential diagnosis.

    Science.gov (United States)

    Lai, Jin-Ping; Robbins, Paul F; Raffeld, Mark; Aung, Phyu Phyu; Tsokos, Maria; Rosenberg, Steven A; Miettinen, Markku M; Lee, Chyi-Chia Richard

    2012-06-01

    A promising targeted therapy against NY-ESO-1 (CTAG 1B) using genetically modified T-cells in synovial sarcomas was recently demonstrated in a clinical trial at the NCI. To investigate the role of NY-ESO-1 immunohistochemistry in patient selection and gain better insight into the incidence of NY-ESO-1 expression in synovial sarcomas and other mesenchymal tumors, we evaluated NY-ESO-1 expression by immunohistochemistry in 417 tumors. This collection of samples included: 50 SS18/SSX1/2 fusion positive synovial sarcomas, 155 gastrointestinal stromal tumors (GIST), 135 other spindle cell sarcomas as well as 77 other sarcomas (chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, rhabdomyosarcoma, angiosarcoma, malignant mesothelioma, and Ewing's sarcoma). We report that 76% of synovial sarcomas expressed NY-ESO-1 in a strong and diffuse pattern (2-3+, >50-70% of tumor cells). In contrast, only rare cases of other spindle cell mesenchymal tumor expressed NY-ESO-1 (GIST (2/155), malignant peripheral nerve sheath tumors (1/34), and dermatofibrosarcoma protuberans (2/20)). Individual cases of other sarcomas (angiosarcoma, malignant mesothelioma, chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, and Ewing's sarcoma) were positive for NY-ESO-1. However, no positive cases were identified amongst our cohort of leiomyosarcomas (0/24), hemangiopericytoma/solitary fibrous tumors (0/40), and cellular schwannomas (0/17). In summary, we find that NY-ESO-1 is strongly and diffusely expressed in a majority of synovial sarcomas, but only rarely in other mesenchymal lesions. Beyond its role in patient selection for targeted therapy, immunohistochemistry for NY-ESO-1 may be diagnostically useful for the distinction of synovial sarcoma from other spindle cell neoplasms.

  12. NY-ESO-1 expression predicts an aggressive phenotype of ovarian cancer.

    Science.gov (United States)

    Szender, J Brian; Papanicolau-Sengos, Antonios; Eng, Kevin H; Miliotto, Anthony J; Lugade, Amit A; Gnjatic, Sacha; Matsuzaki, Junko; Morrison, Carl D; Odunsi, Kunle

    2017-06-01

    NY-ESO-1 is a cancer testis antigen and a promising target for immunotherapy. The purpose of this study was to determine the expression frequency, immunogenicity, and clinical impact of NY-ESO-1 in ovarian cancer. Immunohistochemistry (IHC), reverse-transcription polymerase chain reaction (RT-PCR), and quantitative-PCR (qRT-PCR) were utilized in an ovarian cancer (including Fallopian tube and primary peritoneal cancers) patient cohort; humoral responses against NY-ESO-1 were determined by ELISA. Clinicopathologic outcomes including progression-free (PFS) and overall (OS) survival were evaluated based on NY-ESO-1 expression. Cohen's kappa (κ) tested agreement between expression tests. NY-ESO-1 expression was detected by any method in 40.7% of 1002 patients' tumors (NY-ESO-1+) and baseline humoral response was identified in 19.0% of 689 tested patients. NY-ESO-1+ patients were older (pESO-1+ patients. A subset analysis of NY-ESO-1+ patients that received immunotherapy demonstrated improved OS by >2years (52.6 vs. 27.2months, pESO-1 expression and an aggressive cancer phenotype. The relatively high expression frequency of NY-ESO-1 in ovarian cancer patients coupled with the poor clinical outcomes in NY-ESO-1+ patients reveals an underappreciated need for targeted therapy against this antigen. In support, our study reveals that NY-ESO-1+ patients enrolled on immunotherapy trials targeting the antigen exhibited an improvement in OS. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. NOAA Office for Coastal Management Benthic Habitat Data, Long Island Sound, Jamaica Bay, and Lower Bay of NY/NJ Harbor, NY, 1994-2002 (NODC Accession 0089467)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data are a collection of benthic habitat data from studies conducted in the coastal Long Island Sound, NY region in GIS shapefile (.shp, .dbf, .shx, and .prj...

  14. Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary

    Science.gov (United States)

    Buró, Botond; Lóki, József; Sipos, György; Négyesi, Gábor; Andrási, Bence; Jakab, Attila; Félegyházi, Enikő; Molnár, Mihály

    2017-04-01

    Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary The Nyírség is an alluvial fan, was built by rivers, which were flown down from the NE Carpathians. When the weather was dry, wind-blown sand was blowed out and the wind started to develop the aeolian landforms. The first significant sand movements in the Nyírség was in the Upper plenniglacial and the Late glacial. The main landforms of the Nyírség were developed at this time. The aeolic transformation of the land was not completed in the Nyírség at the end of the Pleistocene. In the Holocene the sand moved within small area, mainly by anthropogenic impact. Our aim is clarify the age of the wind-blown sand movements period, with different absolute (Radiocarbon dating method, OSL), and relative (Archaeological finds, Pollen analyses) dating methods. We have collected for the age determining charcoal from many sand quarry (Gégény, Kántorjánosi, Nyíradony, Nagyvarsány, Máriapócs and Lövőpetri) which contain fossil soil layer. For the OSL measurements samples were collected from Baktalórántháza, Gégény and Kántorjánosi sand quarries. We collected samples from 5 places for the pollen analyses (Nyírtanya, Máriapócs, Nyírbéltek-Nyírlúgos, Nyírábrány, Vámospércs), and also from an archaeological excavation (Nyíregyháza- Oros). The new age dates show us, that in the Nyírség the first significant sand movements were in the cold and drier period of the Upper pleniglacial and Late glacial (Baktalórántháza, Nyírtanya, Vámospércs). At the end of the Pleistocene the sand movements not competed in the Nyírség (Gégény, Kántorjánosi, Nyírábrány). In the Holocene there were many little sand movement periods, mainly caused by anthropogenic impact (Nyíradony, Nyíregyháza-Oros). The research was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund in the project of GINOP-2.3.2.-15-2016-00009 'ICER

  15. 78 FR 35596 - Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY

    Science.gov (United States)

    2013-06-13

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY AGENCY: Coast Guard, DHS. ACTION: Notice of Proposed Rulemaking... Atlantic Ocean off Long Beach, NY during the Long Beach Regatta Powerboat Race scheduled for August 24-25...

  16. 33 CFR 207.600 - Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation. 207.600 Section 207.600 Navigation and Navigable Waters CORPS OF... (Charlotte) Harbor, N.Y.; use, administration, and navigation. (a)-(b) (c) No vessel shall moor or anchor to...

  17. 33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation. 207.580 Section 207.580 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use...

  18. 33 CFR 207.50 - Hudson River Lock at Troy, N.Y.; navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Hudson River Lock at Troy, N.Y..., DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.50 Hudson River Lock at Troy, N.Y.; navigation. (a... thereof, and passing coal from flats or barges to a steamboat while in the lock is prohibited. (m) (n...

  19. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Science.gov (United States)

    2010-07-01

    ... Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.156 Randall Bay, Freeport, Long Island, N.Y. (a) The anchorage grounds. Southward of a line 312 feet south of and parallel to the south side...

  20. 77 FR 39406 - Safety Zone; Tom Graves Memorial Fireworks, Port Bay, Wolcott, NY

    Science.gov (United States)

    2012-07-03

    ...-AA00 Safety Zone; Tom Graves Memorial Fireworks, Port Bay, Wolcott, NY AGENCY: Coast Guard, DHS. ACTION..., NY. This safety zone is intended to restrict vessels from a portion of Port Bay during the Tom Graves... necessary to ensure the safety of spectators and vessels during the Tom Graves Memorial Fireworks. This zone...

  1. 76 FR 6728 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Science.gov (United States)

    2011-02-08

    ...-AA00 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The Coast Guard proposes to establish a permanent safety zone.... 165.171 to read as follows: Sec. 165.171 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY...

  2. NY-ESO-1-specific immunological pressure and escape in a patient with metastatic melanoma.

    Science.gov (United States)

    von Boehmer, Lotta; Mattle, Muriel; Bode, Peter; Landshammer, Alexandro; Schäfer, Carolin; Nuber, Natko; Ritter, Gerd; Old, Lloyd; Moch, Holger; Schäfer, Niklaus; Jäger, Elke; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    During cancer progression, malignant cells may evade immunosurveillance. However, evidence for immunological escape in humans is scarce. We report here the clinical course of a melanoma patient whose initial tumor was positive for the antigens NY-ESO-1, MAGE-C1, and Melan-A. Upon immunization with a recombinant vaccinia/fowlpox NY-ESO-1 construct, the patient experienced a mixed clinical response and spreading of the NY-ESO-1 epitopes in the CD4+ T cell compartment. After NY-ESO-1 protein + CpG immunization, the patient's anti-NY-ESO-1 IgG response increased. Over the following years, progressing lesions were resected and found to be NY-ESO-1-negative while being positive for MAGE-C1, Melan-A, and MHC-I. The fatal, inoperable brain metastasis was analyzed after his death and also proved to be NY-ESO-1-negative, while being positive for MAGE-C1 and Melan-A, as well as MHC-I. We propose that cancer control and cancer escape in this patient were governed by NY-ESO-1-specific immunological pressure. Our findings provide evidence for the existence of immunoediting and immunoescape in this cancer patient.

  3. Expression and immunogenicity of NY-ESO-1 in colorectal cancer.

    Science.gov (United States)

    Li, Ya; Song, Ruifeng; Li, Xinqiang; Xu, Feng

    2017-06-01

    Serum assays of NY-ESO-1 antibodies provide a guide to discriminate between patients who suffer from different types of cancer. In the present study, the expression of NY-ESO-1 was detected with the aim to identify a novel tumor antigen in colorectal cancer (CRC). Sera were obtained from 89 healthy individuals and 236 patients with CRC with stage I, II, III and IV tumors. The NY-ESO-1 autoantibody levels were determined using an enzyme-linked immunosorbent assay. The mRNA and protein expression levels of NY-ESO-1 were detected using reverse transcription-polymerase chain reaction and immunohistochemistry, respectively, in 60 CRC and paired adjacent non-tumor tissues. NY-ESO-1 antibody was detected in 40 of the 236 (16.9%) patients with CRC. The NY-ESO-1 antibody combined with carcinoembryonic antigen enhanced the sensitivity, from 52.1 to 62.7%, of the diagnosis of CRC. The frequency of antibody positivity increased with the TNM cancer stage (8.8 vs. 28.3% in stages I+II and III+IV, respectively). The mRNA and protein expression levels of NY-ESO-1 were significantly higher in CRC tissue than in adjacent non-tumor tissue. In conclusion, NY-ESO-1 is frequently expressed in CRC with the capacity of inducing a humoral immune response in CRC patients, exhibiting the potential to be a promising biomarker for CRC.

  4. Epigenetic potentiation of NY-ESO-1 vaccine therapy in human ovarian cancer.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; James, Smitha R; Mhawech-Fauceglia, Paulette; Tsuji, Takemasa; Miller, Austin; Zhang, Wa; Akers, Stacey N; Griffiths, Elizabeth A; Miliotto, Anthony; Beck, Amy; Batt, Carl A; Ritter, Gerd; Lele, Shashikant; Gnjatic, Sacha; Karpf, Adam R

    2014-01-01

    The cancer-testis/cancer-germline antigen NY-ESO-1 is a vaccine target in epithelial ovarian cancer (EOC), but its limited expression is a barrier to vaccine efficacy. As NY-ESO-1 is regulated by DNA methylation, we hypothesized that DNA methyltransferase (DNMT) inhibitors may augment NY-ESO-1 vaccine therapy. In agreement, global DNA hypomethylation in EOC was associated with the presence of circulating antibodies to NY-ESO-1. Pre-clinical studies using EOC cell lines showed that decitabine treatment enhanced both NY-ESO-1 expression and NY-ESO-1-specific CTL-mediated responses. Based on these observations, we performed a phase I dose-escalation trial of decitabine, as an addition to NY-ESO-1 vaccine and doxorubicin liposome (doxorubicin) chemotherapy, in 12 patients with relapsed EOC. The regimen was safe, with limited and clinically manageable toxicities. Both global and promoter-specific DNA hypomethylation occurred in blood and circulating DNAs, the latter of which may reflect tumor cell responses. Increased NY-ESO-1 serum antibodies and T cell responses were observed in the majority of patients, and antibody spreading to additional tumor antigens was also observed. Finally, disease stabilization or partial clinical response occurred in 6/10 evaluable patients. Based on these encouraging results, evaluation of similar combinatorial chemo-immunotherapy regimens in EOC and other tumor types is warranted.

  5. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    Science.gov (United States)

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  6. 75 FR 38710 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-07-06

    ..., Huntington Bay, NY AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard is establishing a permanent Special Local Regulation on the navigable waters of Huntington Bay, New York due to the annual... Championships, Huntington Bay, NY'' in the Federal Register (75 FR 13454). The Coast Guard received no comments...

  7. 75 FR 13454 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-03-22

    ... Championships, Huntington Bay, NY AGENCY: Coast Guard, DHS. ACTION: Supplemental Notice of proposed rulemaking... Special Local Regulation on the navigable waters of Huntington Bay, New York due to the annual Fran... championship swim on the waters of Huntington Bay, NY during a single day in July. This swim has historically...

  8. 33 CFR 207.60 - Federal Dam, Hudson River, Troy, N.Y.; pool level.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Federal Dam, Hudson River, Troy..., DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.60 Federal Dam, Hudson River, Troy, N.Y.; pool level. (a) Whenever the elevation of the pool created by the Federal dam at Troy, N.Y...

  9. 77 FR 1023 - Regulated Navigation Area; Arthur Kill, NY and NJ

    Science.gov (United States)

    2012-01-09

    ... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2011-0727] RIN 1625-AA11 Regulated Navigation Area; Arthur Kill, NY and NJ AGENCY: Coast Guard, DHS. ACTION: Temporary... Navigation Area; Arthur Kill, NY and NJ. * * * * * (b) * * * (9) Suspension of enforcement: the Captain of...

  10. Paradise Papers bør føre til en ny global skat

    DEFF Research Database (Denmark)

    Christensen, Rasmus Corlin; Stausholm, Saila Naomi

    2017-01-01

    Det nytter ikke med lappeløsninger. Skal vi sætte en stopper for skattely, kræver det internationalt samarbejde en helt ny form for skat.......Det nytter ikke med lappeløsninger. Skal vi sætte en stopper for skattely, kræver det internationalt samarbejde en helt ny form for skat....

  11. 33 CFR 110.150 - Block Island Sound, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Block Island Sound, N.Y. 110.150 Section 110.150 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A 3/4...

  12. 75 FR 33327 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. Notice... the New York University College of Dentistry, New York, NY. The human remains were removed from the... College of Dentistry professional staff in consultation with representatives of the Caddo Nation of...

  13. 75 FR 33329 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. Notice... the New York University College of Dentistry, New York, NY. The human remains were removed from.... A detailed assessment of the human remains was made by the New York University College of Dentistry...

  14. 75 FR 36110 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-24

    ... University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. Notice... the New York University College of Dentistry, New York, NY. The human remains were removed from... College of Dentistry professional staff in consultation with representatives of the Alabama-Quassarte...

  15. 76 FR 34865 - Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY

    Science.gov (United States)

    2011-06-15

    ...-AA00 Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY AGENCY: Coast Guard, DHS... Genesee River, Rochester, NY for the Rochester Harbor Festival fireworks. This zone is intended to restrict vessels from the mouth of the Genesee River in Rochester during the Rochester Harbor Festival...

  16. 77 FR 60044 - Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY

    Science.gov (United States)

    2012-10-02

    ... 1625-AA00 Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY AGENCY: Coast... navigable waters of Patchogue Bay, in Patchogue, NY for the DeStefano family wedding fireworks display. This..., 2012 we published a notice of proposed rulemaking (NPRM) entitled Safety Zone; DeStefano Wedding...

  17. Kvalitetsmålinger i almen praksis: ny teknologi og gamle logikker

    DEFF Research Database (Denmark)

    Lippert, Maria Laura

    2011-01-01

    ny teknologi p† den ene side stilles overfor forstening og teknologiforskr‘kkelse p† den anden. Det er en modstilling, som denne artikel sigter mod at nuancere ved at s‘tte datafangstinitiativet ind i en evalueringshistorisk sammenh‘ng. Et tilbageblik viser, at selvom det er udviklingen af helt ny it...

  18. Allegheny County TIF Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Tax Increment Financing (TIF) outline parcels for Allegheny County, PA. TIF closing books contain all necessary documentation related to a TIF in order to close on...

  19. Allegheny County Greenways

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Greenways data was compiled by the Allegheny Land Trust as a planning effort in the development of Allegheny Places, the Allegheny County Comprehensive Plan. The...

  20. Allegheny County Parcel Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains parcel boundaries attributed with county block and lot number. Use the Property Information Extractor for more control downloading a filtered...

  1. Allegheny County Parks Outlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Shows the size and shape of the nine Allegheny County parks. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  2. Allegheny County Dam Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the point locations of dams in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  3. Allegheny County Plumbers

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — All master plumbers must be registered with the Allegheny County Health Department. Only Registered Master Plumbers who possess a current plumbing license or...

  4. Allegheny County Hospitals

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The data on health care facilities includes the name and location of all the hospitals and primary care facilities in Allegheny County. The current listing of...

  5. Allegheny County Boundary

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains the Allegheny County boundary. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  6. Allegheny County Employee Salaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Employee salaries are a regular Right to Know request the County receives. Here is the disclaimer language that is included with the dataset from the Open Records...

  7. Allegheny County Crash Data

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Contains locations and information about every crash incident reported to the police in Allegheny County from 2004 to 2016. Fields include injury severity,...

  8. Allegheny County Tobacco Vendors

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The tobacco vendor information provides the location of all tobacco vendors in Allegheny County in 2015. Data was compiled from administrative records managed by...

  9. Allegheny County Traffic Counts

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Traffic sensors at over 1,200 locations in Allegheny County collect vehicle counts for the Pennsylvania Department of Transportation. Data included in the Health...

  10. Beaver County Crash Data

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Contains locations and information about every crash incident reported to the police in Beaver County from 2011 to 2015. Fields include injury severity, fatalities,...

  11. Taos County Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Vector line shapefile under the stewardship of the Taos County Planning Department depicting roads in Taos County, New Mexico. Originally under the Emergency...

  12. County Population Vulnerability

    Data.gov (United States)

    City and County of Durham, North Carolina — This layer summarizes the social vulnerability index for populations within each county in the United States at scales 1:3m and below. It answers the question...

  13. Allegheny County Depression Medication

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — These Census Tract-level datasets described here provide de-identified diagnosis data for customers of three managed care organizations in Allegheny County (Gateway...

  14. Allegheny County Anxiety Medication

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — These Census Tract-level datasets described here provide de-identified diagnosis data for customers of three managed care organizations in Allegheny County (Gateway...

  15. Butler County Crash Data

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Contains locations and information about every crash incident reported to the police in Butler County from 2011 to 2015. Fields include injury severity, fatalities,...

  16. Allegheny County Property Viewer

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Webmap of Allegheny municipalities and parcel data. Zoom for a clickable parcel map with owner name, property photograph, and link to the County Real Estate website...

  17. Allegheny County Property Assessments

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Real Property parcel characteristics for Allegheny County, PA. Includes information pertaining to land, values, sales, abatements, and building characteristics (if...

  18. Allegheny County Street Centerlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains the locations of the street centerlines for vehicular and foot traffic in Allegheny County. Street Centerlines are classified as Primary Road,...

  19. Allegheny County Major Rivers

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of major rivers that flow through Allegheny County. These shapes have been taken from the Hydrology dataset. The Ohio River,...

  20. Allegheny County Asbestos Permits

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Current asbestos permit data issued by the County for commercial building demolitions and renovations as required by the EPA. This file is updated daily and can be...

  1. Allegheny County Obesity Rates

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Obesity rates for each Census Tract in Allegheny County were produced for the study “Developing small-area predictions for smoking and obesity prevalence in the...

  2. Allegheny County Smoking Rates

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Smoking rates for each Census Tract in Allegheny County were produced for the study “Developing small-area predictions for smoking and obesity prevalence in the...

  3. Methane Gas Utilization Project from Landfill at Ellery (NY)

    Energy Technology Data Exchange (ETDEWEB)

    Pantelis K. Panteli

    2012-01-10

    Landfill Gas to Electric Energy Generation and Transmission at Chautauqua County Landfill, Town of Ellery, New York. The goal of this project was to create a practical method with which the energy, of the landfill gas produced by the decomposing waste at the Chautauqua County Landfill, could be utilized. This goal was accomplished with the construction of a landfill gas to electric energy plant (originally 6.4MW and now 9.6MW) and the construction of an inter-connection power-line, from the power-plant to the nearest (5.5 miles) power-grid point.

  4. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

    Science.gov (United States)

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H; Kalos, Michael D; Grupp, Stephan A

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastoma samples and demonstrated that 23% express NY-ESO-1. After confirming antigen-specific activity of T cells genetically engineered to express an NY-ESO-1 directed high-affinity transgenic T cell receptor in vitro , we performed xenograft mouse studies assessing the efficacy of NY-ESO-1-targeted T cells in both localized and disseminated models of neuroblastoma. Disease responses were monitored by tumor volume measurement and in vivo bioluminescence. After delivery of NY-ESO-1 transgenic TCR T cells, we observed significant delay of tumor progression in mice bearing localized and disseminated neuroblastoma, as well as enhanced animal survival. These data demonstrate that NY-ESO-1 is an antigen target in neuroblastoma and that targeted T cells represent a potential therapeutic option for patients with neuroblastoma.

  5. Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers.

    Science.gov (United States)

    Park, Tristen S; Groh, Eric M; Patel, Krishna; Kerkar, Sid P; Lee, Chyi-Chia Richard; Rosenberg, Steven A

    2016-01-01

    Melanoma-associated antigen-A (MAGE-A) and New York esophageal squamous cell cancer-1 (NY-ESO-1) are 2 cancer testis antigens (CTA) demonstrating potential for use in targeted immunotherapy. Clinical trials in melanoma and synovial sarcomas targeting these antigens in immune-based therapies have demonstrated durable tumor regression. Although protein expression of NY-ESO-1 has been assessed in a variety of cancer types, the expression of MAGE-A has not been studied in depth. In this study we analyzed MAGE-A and NY-ESO-1 expression in 314 melanoma specimens from 301 melanoma patients, 38 patients with squamous cell cancers and 111 patients with adenocarcinomas. Our results demonstrated higher expression of MAGE-A compared with NY-ESO-1 in melanomas (32% vs. 13%) and squamous cell carcinomas (45% vs. 7.9%), and higher expression of both CTAs in metastatic versus primary tumors. CTA expression in adenocarcinomas was low (MAGE-A: 10%, NY-ESO-1: 0.9%). In addition, we looked at concordance of expression among metastatic melanoma lesions within the same patient and found concordant expression in 38 of 47 patients for MAGE-A and 43 of 47 patients for NY-ESO-1. Our study demonstrated that the MAGE-A family may be of greater utility than NY-ESO-1 for targeted immunotherapy in a variety of cancer histologies, in particular metastatic melanomas and squamous cell carcinomas.

  6. Application of serum NY-ESO-1 antibody assay for early SCLC diagnosis.

    Science.gov (United States)

    Yang, Jihua; Jiao, Shunchang; Kang, Jingbo; Li, Rong; Zhang, Guanzhong

    2015-01-01

    NY-ESO-1 antibody is one of the cancer-related antibodies. The purpose of this study was to investigate the diagnostic role of the NY-ESO-1 humoral immune response in small cell lung cancer (SCLC). We recombined the recombinant protein of NY-ESO-1 antibody and NSE, analyzed them by Enzyme-linked immunosorbent assay, and then established the Receiver Operating Characteristic (ROC) curve to estimate the diagnostic value of NY-ESO-1 antibody, NSE and their combinations. According to detection, the positive rate of NY-ESO-1 humoral immune response (26.3%), NSE (43.8%) and their combinations (10.5%) were all lower than the negative rate which indicated that the NY-ESO-1 antibody might be down-regulated in SCLC. And the positive rate wasn't related to clinicopathologic characteristics. The ROC curve demonstrated that with a 37.17% sensitivity and a 91.7% specificity along with a AUC of 0.619 for NY-ESO-1ab as well as with a 48.3% sensitivity and a 90.87% specificity along with a AUC value of 0.773 for NSE, their diagnostic value were both high. Besides, the diagnostic value of their combinations was also good for a AUC of 0.83 and a 69.12% sensitivity and a 91.8% specificity. There were significant difference of diagnostic value among three types above (NY-ESO-1 vs. NSE, P ESO-1, P ESO-1ab, NSE and their combinations all were important diagnostic markers for SCLC. Moreover, the diagnostic value of their combinations was higher than any single of them. And NY-ESO-1 humoral immune to NSE might be a potential diagnostic indicator in SCLC.

  7. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    Science.gov (United States)

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer.

  8. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery.

    Science.gov (United States)

    Xu, Heng; Gu, Na; Liu, Zhao-Bo; Zheng, Min; Xiong, Fang; Wang, Si-Ying; Li, Ning; Lu, Jun

    2012-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for this correlation. A total of 120 HCC patients were analyzed for the expression of NY-ESO-1 by immunohistochemistry (IHC). A stable NY-ESO-1 over-expressed HepG2 cell line (ESO-HepG2) was established to determine the biological effects of NY-ESO-1 on cell proliferation, cell cycle and migration by using the xCELLigence DP system, flow cytometry and xCELLigence SP system. NY-ESO-1 was positive in 28 of 120 (23.3%) HCC tumor tissues. NY-ESO-1 was not detectable in adjacent normal liver tissues. A close correlation was found between NY-ESO-1 expression and the recurrence of HCC following surgery (P=0.007). Kaplan-Meier analysis showed a shorter recurrence-free survival (RFS) for patients positive for NY-ESO-1 (log-rank test, P=0.003). The Cox regression model demonstrated that NY-ESO-1 expression was a significant independent predictor for the recurrence of HCC following curative surgery (P=0.022). Compared with HepG2 cells, ESO-HepG2 cells have increased migration but not proliferation ability. In conclusion, NY-ESO-1 expression is associated with worse HCC outcome following surgery, and the mechanism for this finding may be that NY-ESO-1 increases tumor cell migration.

  9. 77 FR 5743 - Special Anchorage Areas; Port of New York, NY

    Science.gov (United States)

    2012-02-06

    ... along the shoreline they fall within the jurisdiction of the States of New Jersey and New York. Both New... special anchorage areas in Sheepshead Bay, NY. We expect minimal additional cost impacts on the tug and...

  10. EnviroAtlas - New York, NY - Meter-Scale Urban Land Cover (MULC) Data (2008)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The New York, NY EnviroAtlas Meter-scale Urban Land Cover (MULC) Data were generated by the University of Vermont Spatial Analysis Laboratory (SAL) under the...

  11. NOAA Digital Orthophotography for the Coasts of Connecticut and Long Island, NY

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Project: NOAA Digital Orthophotography for the Coasts of Main/New Hampshire, Massachusetts/Rhode Island/Connecticut, and Hudson River/Long Island /NY/NJ Contract No....

  12. 33 CFR 162.15 - Manhasset Bay, N.Y.; seaplane restricted area.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Manhasset Bay, N.Y.; seaplane... Bay, N.Y.; seaplane restricted area. (a) The restricted area. An area in Manhasset Bay between the... line bearing 166°50′ true from latitude 40°50′17.337 N, longitude 73°43′03.877 W, which point is on the...

  13. NY-ESO-1 expression is tightly linked to TMPRSS2-ERG fusion in prostate cancer.

    Science.gov (United States)

    Grupp, Katharina; Ospina-Klinck, Daniel; Tsourlakis, Maria Christina; Koop, Christina; Wilczak, Waldemar; Adam, Meike; Simon, Ronald; Sauter, Guido; Izbicki, Jakob Robert; Graefen, Markus; Huland, Hartwig; Steurer, Stefan; Schlomm, Thorsten; Minner, Sarah; Quaas, Alexander

    2014-07-01

    NY-ESO-1 has been suggested as therapeutic cancer vaccine in prostate cancer. This study was undertaken to explore the relationship of NY-ESO-1 with tumor phenotype, biochemical recurrence, and molecular subgroups in hormone-naive prostate cancers. NY-ESO-1 immunohistochemistry was analyzed on a tissue microarray containing 11,152 prostate cancer samples. Results were compared to clinically follow-up data, ERG status, and deletions on PTEN, 3p13, 5q21, and 6q15. NY-ESO-1 expression was absent in benign prostate glands. In prostate cancer, NY-ESO-1 positivity was found 8.8% of our 8,761 interpretable tumors including 5.8% with weak, 2.5% with moderate, and 0.5% with strong expression. There was a threefold higher rate of NY-ESO-1 expression in ERG fusion positive tumors than in ERG negative cancers (P ESO-1 expression was associated with early biochemical recurrence (P = 0.0002) and high Gleason grade (P ESO-1 expression was also linked to PTEN (P = 0.0012) and 6q15 deletions (P = 0.0005). Our observations indicate a tight link of NY-ESO-1 expression to ERG activation and (to a lesser extent) PTEN- and 6q15-deletions in prostate cancer. The impact of these interactions on the likelihood of response to immunotherapy is unclear. The prognostic impact of NY-ESO-1 expression is little and not independent of histologic variables. © 2014 Wiley Periodicals, Inc.

  14. 33 CFR 110.85 - Niagara River, Youngstown, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Niagara River, Youngstown, N.Y... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.85 Niagara River, Youngstown, N.Y. (a) Area 1... of the Niagara River at latitude 43°14′33″ N, longitude 79°03′7.5″ W; thence westerly to a point at...

  15. VT Boundaries - county polygons

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The BNDHASH dataset depicts Vermont villages, towns, counties, Regional Planning Commissions (RPC), and LEPC (Local Emergency Planning Committee)...

  16. Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells from CHP-NY-ESO-1-vaccinated patients.

    Science.gov (United States)

    Mizote, Yu; Taniguchi, Taku; Tanaka, Kei; Isobe, Midori; Wada, Hisashi; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Uenaka, Akiko; Nakayama, Eiichi

    2010-07-19

    Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100, DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination. (c) 2010 Elsevier Ltd. All rights reserved.

  17. NY-ESO-1 as a potential immunotherapeutic target in renal cell carcinoma.

    Science.gov (United States)

    Giesen, Eva; Jilaveanu, Lucia B; Parisi, Fabio; Kluger, Yuval; Camp, Robert L; Kluger, Harriet M

    2014-07-30

    Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. We employed tissue microarrays containing >300 unique RCC cases and adjacent benign renal tissue to determine NY-ESO-1 expression using a quantitative immunofluorescence method. In addition, we studied NY-ESO-1 expression in 35 matched primary and metastatic RCC specimens to assess concordance between different tumor sites. NY-ESO-1 was highly expressed in a subset of RCCs. Expression in primary RCC specimens was significantly higher than adjacent normal renal tissue (PESO-1 expression seen in clear cell RCC suggests that NY-ESO-1 targeting approaches should be studied in this disease. Expression is higher in metastatic sites, and discordance between primary and metastatic sites in some patients suggests that patient selection for these therapies should be based on expression in metastatic rather than nephrectomy specimens.

  18. NY-ESO-1 (CTAG1B) expression in mesenchymal tumors.

    Science.gov (United States)

    Endo, Makoto; de Graaff, Marieke A; Ingram, Davis R; Lim, Simin; Lev, Dina C; Briaire-de Bruijn, Inge H; Somaiah, Neeta; Bovée, Judith V M G; Lazar, Alexander J; Nielsen, Torsten O

    2015-04-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies. We performed a large-scale immunohistochemical expression study of NY-ESO-1 using tissue microarrays of mesenchymal tumors from three institutions in an international collaboration. A total of 1132 intermediate and malignant and 175 benign mesenchymal lesions were enrolled in this study. Immunohistochemical staining was performed on tissue microarrays using a monoclonal antibody for NY-ESO-1. Among mesenchymal tumors, myxoid liposarcomas showed the highest positivity for NY-ESO-1 (88%), followed by synovial sarcomas (49%), myxofibrosarcomas (35%), and conventional chondrosarcomas (28%). Positivity of NY-ESO-1 in the remaining mesenchymal tumors was consistently low, and no immunoreactivity was observed in benign mesenchymal lesions. On the basis of these findings, nearly 90% of myxoid liposarcomas, as well as a significant proportion of synovial sarcomas, myxofibrosarcomas, and conventional chondrosarcomas are good candidates for immunotherapy targeting NY-ESO-1.

  19. Atmospheric Research in Ny-Aalesund. Proceedings from the Third NySMAC meeting. NILU, Kjeller, Norway, 9.-11. April 1997

    Energy Technology Data Exchange (ETDEWEB)

    Floeisand, Inga; Gernandt, Hartwig; Larsen, Elisabeth Stoltz; Stordal, Frode; Wada, Makoto

    1997-07-01

    Leading abstract. The Ny-Aalesund Seminar Series was conceived by NySMAC (Ny-Aalesund Science Managers Committee) as a means of bringing together research scientists involved in programmes running at Ny-Aalesund or having an interest in that area. The first Seminar was held in Potsdam in Germany in May 1995, and included talks which covered all current research disciplines; physical, atmospheric, earth and biological sciences. The Second Seminar was devoted to biological science and particularly to ecology. It was held in Cambridge in UK in February 1996. The subject of this Third Seminar, held in Kjeller in Norway in April 1997, is atmospheric research in Ny-Aalesund. Since the early 1980s a wide range of projects have been carried out on Svalbard and particularly at Ny-Aalesund. Prominent in this research have been several international research programmes with emphasis on topics of e.g. Arctic (Arctic Monitoring and Assessment Programme, AMAP), European (European Monitoring and Evaluation Programme, EMEP) or global (International Geosphere-Biosphere Programme, IGBP; World Climate Research Programme, WCRP) scales. Atmospheric observations have been carried out in Ny-Aalesund in Svalbard for several years. From the early 1980s, the long range transport of sulphur dioxide and sulphate aerosols leading to Arctic Haze, has been studied. As a consequence, EMEP included Ny-Aalesund in their network. In the following years the transport of ozone and chemical precursors were observed, and recently the naturally occurring phenomenon of rapid surface ozone loss has been given particular attention. From the late 1980s observations of the stratospheric ozone layer and related chemical species were undertaken, and the efforts have increased considerably since. Ny-Aalesund is now one of the primary Arctic sites of the NDSC (Network for Detection of Stratospheric Change) network. Important efforts have been undertaken to participate in the European campaigns as the European

  20. Technical assessment of fuel cell operation on anaerobic digester gas at the Yonkers, NY, wastewater treatment plant.

    Science.gov (United States)

    Spiegel, R J; Preston, J L

    2003-01-01

    This paper summarizes the results of a 2-year field test to assess the performance of a specially modified commercial phosphoric acid 200-kW fuel cell power plant to recover energy from anaerobic digester gas (ADG) which has been cleansed of contaminants (sulfur and halide compounds) using a patented gas pretreatment unit (GPU). Specific project goals include characterization of the fuel cell power plant emissions and verification of the GPU performance for removing sulfur contaminants. To remove halide contaminants from the ADG, a halide guard, consisting of a vessel with a metal oxide supported on alumina, was incorporated into the fuel cell reactant supply. This first-of-a-kind demonstration was conducted at the Yonkers, NY, wastewater treatment plant, a sewage processing facility owned and operated by Westchester County. Results have demonstrated that the ADG fuel cell power plant can produce electrical output levels close to full power (200 kW) with negligible air emissions of CO, NO(x), and SO(2). The GPU removed virtually 100% of H(2)S and 88% of organic sulfur, bringing the overall sulfur removal efficiency of the GPU to over 99%. The halide guard removed up to 96% of the halides exiting the GPU.

  1. [Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli].

    Science.gov (United States)

    Tang, Lei; Song, Chao-jun; Sun, Yuan-jie; Li, Na; Wei, Yu-ying; Sun, Yi; Yang, Kun

    2012-10-01

    To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E.coli. NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-1 downstream tagged by GST to construct the expression plasmid pGEX-4T1-NY-ESO-1. The recombinant vector was transformed to BL21 (DE3) and NY-ESO-1/GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-4T1-NY-ESO-1 expressed a M(r); 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.

  2. Allegheny County Blazed Trails Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Shows the location of blazed trails in all Allegheny County parks. This is the same data used in the Allegheny County Parks Trails Mobile App, available for Apple...

  3. Allegheny County Supermarkets & Convenience Stores

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Location information for all Supermarkets and Convenience Stores in Allegheny County was produced using the Allegheny County Fee and Permit Data for 2016.

  4. Matter of 381 Search Warrants Directed to Facebook, 2017 N.Y. Lexis 767, 2; 2017 NY Slip op. 02586, 2 (N.Y. April 4, 2017

    Directory of Open Access Journals (Sweden)

    Trad. Leopoldo Villar

    2017-08-01

    Full Text Available The growing importance of electronic communications gives a special interest to its protection, which involves several fundamental rights and guarantees such as freedom, privacy and property. Due to the importance of the discussion and the different positions it reflects on an issue of such sensitivity and pertinence in the world today, we publish below the translation of the decision by the Supreme Court of the State of New York of April 4, 2017, on the 381 search warrants directed to Facebook user accounts, ordered by the same Court in 2013 in response to a request by the New York County District Attorney, supported in turn by an affidavit given by a prosecutor in a criminal investigation. Facebook filed a motion seeking annulment of the search warrants and disclosure of the Prosecutor’s affidavit but both motions were rejected by the Appeals Division, whereupon Facebook appealed to the New York Supreme Court, which dismissed the appeal. Although most agreed that the appeal was not appropriate, the views of the judges contain interpretations that make their reading very useful and particularly the opinión given by the dissenting judge offers enriching arguments for defending the rights of users of electronic services.

  5. Allegheny County Block Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset overlays a grid on the County to assist in locating a parcel. The grid squares are 3,500 by 4,500 square feet. The data was derived from original...

  6. LANDSLIDES IN SUCEAVA COUNTY

    Directory of Open Access Journals (Sweden)

    Dan Zarojanu

    2017-07-01

    Full Text Available In the county of Suceava, the landslides are a real and permanent problem. This paper presents the observations of landslides over the last 30 years in Suceava County, especially their morphology, theirs causes and the landslide stopping measures. It presents also several details regarding the lanslides from the town of Suceava, of Frasin and the village of Brodina.

  7. Allegheny County Watershed Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates the 52 isolated sub-Watersheds of Allegheny County that drain to single point on the main stem rivers. Created by 3 Rivers 2nd Nature based...

  8. Efficacy of systemic adoptive transfer immunotherapy targeting NY-ESO-1 for glioblastoma.

    Science.gov (United States)

    Everson, Richard G; Antonios, Joseph P; Lisiero, Dominique N; Soto, Horacio; Scharnweber, Rudi; Garrett, Matthew C; Yong, William H; Li, Ning; Li, Gang; Kruse, Carol A; Liau, Linda M; Prins, Robert M

    2016-03-01

    Immunotherapy is an ideal treatment modality to specifically target the diffusely infiltrative tumor cells of malignant gliomas while sparing the normal brain parenchyma. However, progress in the development of these therapies for glioblastoma has been slow due to the lack of immunogenic antigen targets that are expressed uniformly and selectively by gliomas. We utilized human glioblastoma cell cultures to induce expression of New York-esophageal squamous cell carcinoma (NY-ESO-1) following in vitro treatment with the demethylating agent decitabine. We then investigated the phenotype of lymphocytes specific for NY-ESO-1 using flow cytometry analysis and cytotoxicity against cells treated with decitabine using the xCelligence real-time cytotoxicity assay. Finally, we examined the in vivo application of this immune therapy using an intracranially implanted xenograft model for in situ T cell trafficking, survival, and tissue studies. Our studies showed that treatment of intracranial glioma-bearing mice with decitabine reliably and consistently induced the expression of an immunogenic tumor-rejection antigen, NY-ESO-1, specifically in glioma cells and not in normal brain tissue. The upregulation of NY-ESO-1 by intracranial gliomas was associated with the migration of adoptively transferred NY-ESO-1-specific lymphocytes along white matter tracts to these tumors in the brain. Similarly, NY-ESO-1-specific adoptive T cell therapy demonstrated antitumor activity after decitabine treatment and conferred a highly significant survival benefit to mice bearing established intracranial human glioma xenografts. Transfer of NY-ESO-1-specific T cells systemically was superior to intracranial administration and resulted in significantly extended and long-term survival of animals. These results reveal an innovative, clinically feasible strategy for the treatment of glioblastoma. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro

  9. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination.

    Science.gov (United States)

    Kawada, Junji; Wada, Hisashi; Isobe, Midori; Gnjatic, Sacha; Nishikawa, Hiroyoshi; Jungbluth, Achim A; Okazaki, Nami; Uenaka, Akiko; Nakamura, Yurika; Fujiwara, Shinichi; Mizuno, Naoaki; Saika, Takashi; Ritter, Erika; Yamasaki, Makoto; Miyata, Hiroshi; Ritter, Gerd; Murphy, Roger; Venhaus, Ralph; Pan, Linda; Old, Lloyd J; Doki, Yuichiro; Nakayama, Eiichi

    2012-02-01

    NY-ESO-1 is a prototypic cancer/testis antigen. In a recent phase I clinical trial, we vaccinated 13 patients bearing NY-ESO-1-expressing tumors with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1) and showed efficient induction of NY-ESO-1 antibody, and CD4 and CD8 T cell responses using peripheral blood from the patients. In our study, we analyzed heteroclitic serological responses in those patients after vaccination. Serological response against 11 tumor antigens including MAGE-A1, MAGE-A3, MAGE-A4, CT7/MAGEC1, CT10/MAGEC2, CT45, CT46/HORMAD1, SOX2, SSX2, XAGE1B and p53 was examined by enzyme-linked immunosorbent assay (ELISA) using sera from ten vaccinated patients. Expression of tumor antigens was determined by reverse transcription-polymerase chain reaction or immunohistochemistry. Eight of nine patients who showed antibody responses against NY-ESO-1 also showed an antibody response against at least 1 of these 11 tumor antigens after vaccination. In one patient, seven tumor antigens were recognized. Specificity analysis of the antibody response by ELISA using control recombinant proteins and synthetic peptides and by Western blot showed that the response was not against His6-tag and/or bacterial products included in a preparation of CHP-NY-ESO-1 used for vaccination. Thus, heteroclitic serological responses appear to be indicative of the overall immune response against the tumor, and their analysis could be useful for immune monitoring in cancer vaccine. Copyright © 2011 UICC.

  10. NY-ESO-1 expression in meningioma suggests a rationale for new immunotherapeutic approaches.

    Science.gov (United States)

    Baia, Gilson S; Caballero, Otavia L; Ho, Janelle S Y; Zhao, Qi; Cohen, Tzeela; Binder, Zev A; Salmasi, Vafi; Gallia, Gary L; Quinones-Hinojosa, Alfredo; Olivi, Alessandro; Brem, Henry; Burger, Peter; Strausberg, Robert L; Simpson, Andrew J G; Eberhart, Charles G; Riggins, Gregory J

    2013-11-01

    Meningiomas are the most common primary intracranial tumors. Surgical resection remains the treatment of choice for these tumors. However, a significant number of tumors are not surgically accessible, recur, or become malignant, necessitating the repetition of surgery and sometimes radiation. Chemotherapy is rarely used and is generally not recognized as an effective treatment. Cancer/testis (CT) genes represent a unique class of genes, which are expressed by germ cells, normally silenced in somatic cells, but activated in various cancers. CT proteins can elicit spontaneous immune responses in patients with cancer and this feature makes them attractive targets for immunotherapy-based approaches. We analyzed mRNA expression of 37 testis-restricted CT genes in a discovery set of 18 meningiomas by reverse transcription PCR. The overall frequency of expression of CT genes ranged from 5.6% to 27.8%. The most frequently expressed was NY-ESO-1, in 5 patients (27.8%). We subsequently analyzed NY-ESO-1 protein expression in a larger set of meningiomas by immunohistochemistry and found expression in 108 of 110 cases. In some cases, NY-ESO-1 expression was diffused and homogenous, but in most instances it was heterogeneous. Importantly, NY-ESO-1 expression was positively correlated with higher grade and patients presenting with higher levels of NY-ESO-1 staining had significantly worse disease-free and overall survival. We have also shown that NY-ESO-1 expression may lead to humoral immune response in patients with meningioma. Considering the limited treatment options for patients with meningioma, the potential of NY-ESO-1-based immunotherapy should be explored. ©2013 AACR.

  11. National Dam Safety Program. Lake Frederick Dam (Inventory Number NY 769), Orange County, New York. Phase 1 Inspection Report

    Science.gov (United States)

    1980-03-11

    COMPUTATIONSF F . OTHER DATA LAL M- PHASE I REPORT - NATIONAL DAM SAFETY PROGRAMA _ Name of Dam: LAKE FREDERICK DAM (I.D. NO. 769) V - State Located: NEW YORK... abc -ut 230 feet to the right of the spiliway. The conduit runs = underground and outfalls about 75 feet downstream frm the toe of the dar; the

  12. 77 FR 47815 - Foreign-Trade Zone 90-Onondaga County, NY Application for Expansion and Reorganization Under...

    Science.gov (United States)

    2012-08-10

    ...-1983) and expanded on March 12, 1999 (Board Order 1030, 64 FR 14212, 3-24-1999). The current zone includes the following site: Site 1 (22 acres)--Woodard Industrial Park, Steelway Boulevard, Clay. The... as ``magnet'' sites: Proposed Site 2 (339.41 acres)--Clay Business Park, NYS Route 31 and Caughdenoy...

  13. A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Kakimi, Kazuhiro; Isobe, Midori; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Doki, Yuichiro; Nakajima, Jun; Seto, Yasuyuki; Yamatsuji, Tomoki; Naomoto, Yoshio; Shiraishi, Kenshiro; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Oka, Mikio; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi

    2011-12-15

    We conducted a phase I clinical trial of a cancer vaccine using a 20-mer NY-ESO-1f peptide (NY-ESO-1 91-110) that includes multiple epitopes recognized by antibodies, and CD4 and CD8 T cells. Ten patients were immunized with 600 μg of NY-ESO-1f peptide mixed with 0.2 KE Picibanil OK-432 and 1.25 ml Montanide ISA-51. Primary end points of the study were safety and immune response. Subcutaneous injection of the NY-ESO-1f peptide vaccine was well tolerated. Vaccine-related adverse events observed were fever (Grade 1), injection-site reaction (Grade 1 or 2) and induration (Grade 2). Vaccination with the NY-ESO-1f peptide resulted in an increase or induction of NY-ESO-1 antibody responses in nine of ten patients. The sera reacted with recombinant NY-ESO-1 whole protein as well as the NY-ESO-1f peptide. An increase in CD4 and CD8 T cell responses was observed in nine of ten patients. Vaccine-induced CD4 and CD8 T cells responded to NY-ESO-1 91-108 in all patients with various HLA types with a less frequent response to neighboring peptides. The findings indicate that the 20-mer NY-ESO-1f peptide includes multiple epitopes recognized by CD4 and CD8 T cells with distinct specificity. Of ten patients, two with lung cancer and one with esophageal cancer showed stable disease. Our study shows that the NY-ESO-1f peptide vaccine was well tolerated and elicited humoral, CD4 and CD8 T cell responses in immunized patients. Copyright © 2011 UICC.

  14. Allegheny County Older Housing

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Older housing can impact the quality of the occupant's health in a number of ways, including lead exposure, housing quality, and factors that may exacerbate...

  15. Allegheny County Housing Tenure

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Home ownership provides a number of financial, social, and health benefits to American families. Especially in areas with housing price appreciation, home ownership...

  16. Allegheny County Cemetery Outlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Outlines of public and private cemeteries greater than one acre in size. Areas were delineated following a generalized line along the outside edge of the area....

  17. Allegheny County Dog Licenses

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — A list of dog license dates, dog breeds, and dog name by zip code. Currently this dataset does not include City of Pittsburgh dogs.

  18. Allegheny County Sheriff Sales

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — List of properties up for auction at a Sheriff Sale. Datasets labeled "Current" contain this month's postings, while those labeled "Archive" contain a running list...

  19. Allegheny County Vacant Properties

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Mail carriers routinely collect data on address no longer receiving mail due to vacancy. This vacancy data is reported quarterly at census tract geographies in the...

  20. HYDRAULICS, GREER COUNTY, OKLAHOMA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Approximate hydraulic analysis was performed on streams in Greer County, Oklahoma. The approximate analysis was performed in accordance the FEMA G&S. Hydraulic...

  1. Durham County Demographic Profile

    Data.gov (United States)

    City and County of Durham, North Carolina — (a) Includes persons reporting only one race.(b) Hispanics may be of any race, so also are included in applicable race categories. D: Suppressed to avoid disclosure...

  2. Allegheny County Hydrology Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Hydrology Feature Dataset contains photogrammetrically compiled water drainage features and structures including rivers, streams, drainage canals, locks, dams,...

  3. Allegheny County Hydrology Lines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Hydrology Feature Dataset contains photogrammetrically compiled water drainage features and structures including rivers, streams, drainage canals, locks, dams,...

  4. Allegheny County Walk Scores

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Walk Score measures the walkability of any address using a patented system developed by the Walk Score company. For each 2010 Census Tract centroid, Walk Score...

  5. 33 CFR 162.20 - Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area. 162.20 Section 162.20 Navigation and Navigable Waters COAST... NAVIGATION REGULATIONS § 162.20 Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area. (a...

  6. 75 FR 35016 - EDF Industrial Power Services (NY), LLC; Supplemental Notice That Initial Market-Based Rate...

    Science.gov (United States)

    2010-06-21

    ... Energy Regulatory Commission EDF Industrial Power Services (NY), LLC; Supplemental Notice That Initial... supplemental notice in the above-referenced proceeding of EDF Industrial Power Services (NY), LLC's application... the FERC Online links at http://www.ferc.gov . To facilitate electronic service, persons with Internet...

  7. 77 FR 15297 - Proposed Establishment of Class D and E Airspace Amendment of Class E Airspace; East Hampton, NY

    Science.gov (United States)

    2012-03-15

    ... E Airspace; East Hampton, NY AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of... existing Class E airspace at East Hampton, NY, to accommodate the new air traffic control tower at East Hampton Airport. Controlled airspace is necessary for the safety and management of instrument flight rules...

  8. 77 FR 37569 - Establishment of Class D Airspace and Amendment of Class E Airspace; East Hampton, NY

    Science.gov (United States)

    2012-06-22

    ...; East Hampton, NY AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action establishes Class D airspace and amends existing Class E airspace at East Hampton, NY, to accommodate the new mobile airport traffic control tower (ATCT) at East Hampton Airport. Controlled airspace...

  9. 77 FR 9227 - AP Gas & Electric (NY), LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

    Science.gov (United States)

    2012-02-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission AP Gas & Electric (NY), LLC; Supplemental Notice That Initial Market-Based... above-referenced proceeding of AP Gas & Electric (NY), LLC's application for market-based rate authority...

  10. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... of Porter Avenue, Buffalo, N.Y. 110.84 Section 110.84 Navigation and Navigable Waters COAST GUARD... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning at...

  11. 77 FR 42510 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY; Correction

    Science.gov (United States)

    2012-07-19

    ... Inventory Completion: New York University College of Dentistry, New York, NY; Correction AGENCY: National... of human remains under the control of the New York University College of Dentistry, New York, NY. The... Dentistry professional staff in consultation with representatives of the Delaware Nation of Oklahoma...

  12. 78 FR 62293 - Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY

    Science.gov (United States)

    2013-10-15

    ... Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY AGENCY: Coast... zone on the navigable waters of Oyster Bay near Oyster Bay, NY for the Oyster Festival 30th Anniversary... Oyster Festival 30th Anniversary Fireworks Display is scheduled for October 19, 2013 and is one of...

  13. 77 FR 43072 - Public Power & Utility of NY, Inc.; Supplemental Notice That Initial Market-Based Rate Filing...

    Science.gov (United States)

    2012-07-23

    ... Public Power & Utility of NY, Inc.'s application for market-based rate authority, with an accompanying... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER12-2251-000] Public Power & Utility of NY, Inc.; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for...

  14. A potential role for immunotherapy in thyroid cancer by enhancing NY-ESO-1 cancer antigen expression.

    Science.gov (United States)

    Gunda, Viswanath; Frederick, Dennie T; Bernasconi, Maria J; Wargo, Jennifer A; Parangi, Sareh

    2014-08-01

    NY-ESO-1 is one of the most immunogenic members of the cancer/testis antigen family and its levels can be increased after exposure to demethylating and deacetylating agents. This cytoplasmic antigen can serve as a potent target for cancer immunotherapy and yet has not been well studied in differentiated thyroid cancer cells. We studied the baseline expression of NY-ESO-1 messenger RNA and protein before and after exposure to 5-aza-2'-deoxycytidine (DAC) (72 hours) in a panel of thyroid cancer cell lines using quantitative polymerase chain reaction and Western blot. HLA-A2+, NY-ESO-1+ thyroid cell lines were then co-cultured with peripheral blood lymphocytes transduced with NY-ESO-1 specific T-cell receptor (TCR) and assayed for interferon-gamma and Granzyme-B release in the medium. SCID mice injected orthotopically with BCPAP cells were treated with DAC to evaluate for NY-ESO-1 gene expression in vivo. None of the thyroid cancer cell lines showed baseline expression of NY-ESO-1. Three cell lines, BCPAP, TPC-1, and 8505c, showed an increase in NY-ESO-1 gene expression with DAC treatment and were found to be HLA-A2 positive. DAC-treated target BCPAP and TPC-1 tumor cells with up-regulated NY-ESO-1 levels were able to mount an appropriate interferon-gamma and Granzyme-B response upon co-culture with the NY-ESO-1-TCR-transduced peripheral blood lymphocytes. In vivo DAC treatment was able to increase NY-ESO-1 expression in an orthotopic mouse model with BCPAP cells. Our data suggest that many differentiated thyroid cancer cells can be pressed to express immune antigens, which can then be utilized in TCR-based immunotherapeutic interventions.

  15. 33 CFR 100.106 - Freeport Grand Prix, Long Beach, NY.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Freeport Grand Prix, Long Beach... Beach, NY. (a) Regulated area. The regulated area is a trapezoidal area on the coastal Atlantic waters of Long Island to the south of Long Beach, New York. The regulated area is one and one quarter (11/4...

  16. 77 FR 51912 - Safety Zone; Tom Lyons Productions Fireworks, Long Island Sound, Sands Point, NY

    Science.gov (United States)

    2012-08-28

    ... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket Number USCG-2012-0618] RIN 1625-AA00 Safety Zone; Tom Lyons Productions Fireworks, Long Island Sound, Sands Point, NY AGENCY.... 165.T01-0618 to read as follows: Sec. 165.T01-0618 Safety Zone; Tom Lyons Productions Fireworks, Long...

  17. 33 CFR 80.155 - Watch Hill, RI to Montauk Point, NY.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Watch Hill, RI to Montauk Point... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Atlantic Coast § 80.155 Watch Hill, RI to Montauk Point, NY. (a) A line drawn from Watch Hill Light to East Point on Fishers Island. (b) A line...

  18. Optimization of process conditions for the production of TiO2–xNy ...

    Indian Academy of Sciences (India)

    Administrator

    tone to Ti, molar ratio of water to Ti and calcination temperature as the key process factors affecting the roughness of TiO2–xNy film. The adjusted determination coefficient (R2. Adj) of the regression model was 0∙9651, which indicated that the regression model is significant. By analysing the contour plots of response surface ...

  19. 76 FR 31233 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Science.gov (United States)

    2011-05-31

    ...-AA00 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY AGENCY: Coast Guard, DHS. ACTION... maritime public and safety of navigation from recently discovered underwater explosive hazards in Gravesend... published a notice of proposed rulemaking (NPRM) entitled ``Safety Zone; Underwater Hazard, Gravesend Bay...

  20. 76 FR 4529 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Science.gov (United States)

    2011-01-26

    ...-AA00 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the waters of....T01-1126 is added as follows: Sec. 165.T01-1126 Safety Zone; Underwater Hazard, Gravesend Bay...

  1. 78 FR 46855 - Safety Zone; East End Maritime Foundation Fireworks Display, Greenport Harbor, Greenport, NY

    Science.gov (United States)

    2013-08-02

    ... 1625-AA00 Safety Zone; East End Maritime Foundation Fireworks Display, Greenport Harbor, Greenport, NY... temporary rule proposes to establish a safety zone for the East End Maritime Foundation fireworks display....1. 0 2. Add Sec. 165.T01-0600 to read as follows: Sec. 165.T01-0600 Safety Zone; East End Maritime...

  2. New York Bight Study: Report 5, NY Bight Biological Review Program

    Science.gov (United States)

    1994-05-01

    bottom Benthos ..................... 30 Table 5. Common and Scientific Names of Common Species from the NY Bight that Exhibit Thigmotropic and...crustaceans are attracted to structures ( thigmotropism ) or cunrents (rheotropism) for a variety of reasons (e.g., protection from predators, mini- mized...1992). Many fish are naturally attracted to borrow pits due to thigmotropism , rheotropism, higher food abundance, ame- liorated environmental

  3. 77 FR 834 - Noise Exposure Map Update for Albany International Airport, Albany, NY

    Science.gov (United States)

    2012-01-06

    .... 47501 et. seq (Aviation Safety and Noise Abatement Act) and 14 CFR part 150 are in compliance with.... section 47503 of the Aviation Safety and Noise Abatement Act (hereinafter referred to as ``the Act''), an... Federal Aviation Administration Noise Exposure Map Update for Albany International Airport, Albany, NY...

  4. 77 FR 65360 - Grant of Authority for Subzone Status (Centrifugal and Submersible Pumps); Auburn, NY

    Science.gov (United States)

    2012-10-26

    ... Status (Centrifugal and Submersible Pumps); Auburn, NY Pursuant to its authority under the Foreign-Trade... authority to establish a special-purpose subzone at the centrifugal and submersible pump manufacturing and... submersible pumps and related controllers at the Xylem Water Systems U.S.A., LLC, facilities located in Auburn...

  5. NY-ESO-1 expression in DCIS: A new predictor of good prognosis.

    Science.gov (United States)

    Coombes, R Charles; Caballero, Otavia L; Shousha, Sami; Ghaem-Maghami, Sadaf; Woodley-Barker, Laura; Wilhelm-Benartzi, Charlotte S; Neville, A Munro

    2017-03-01

    At present, it is difficult to predict which patients with ductal carcinoma-in-situ (DCIS) will subsequently develop frank invasive breast cancer (IDC). A recent survey by our group has shown that NY-ESO-1 and MAGEA are both expressed in DCIS. This study was aimed at determining whether expression of these antigens was related to the later development of IDC. 14 of 42 (33%) of patients developed invasive breast cancer during the follow up period. Only one of those DCIS cases that relapsed was positive for NYESO-1 at diagnosis. In contrast, DCIS samples of 15 of the 28 (54%) of those patients who remained disease-free expressed NY-ESO-1. (Permutation chi square p=0.0033). We identified 42 patients with DCIS, and followed them up for more than 10 years. NY-ESO-1 and MAGEA were demonstrated by immunostaining as were CD8+ infiltrates on all sections together with the conventional markers, ER, PR, and HER2. Expression of NY-ESO-1 may predict those patients who will not subsequently develop invasive breast cancer and could therefore potentially be helpful in defining prognosis in patients with DCIS.

  6. 75 FR 65523 - Dawson Metal Company, Inc., Industrial Division, Jamestown, NY; Notice of Negative Determination...

    Science.gov (United States)

    2010-10-25

    ...., Industrial Division, Jamestown, NY; Notice of Negative Determination on Reconsideration On January 21, 2010... the relatively insignificant scale of the customer's decline. The fourth customer was the customer... adjustment assistance for workers and former workers of Dawson Metal Company, Inc., Industrial Division...

  7. En ny vej at gå for udsatte unge i USA

    DEFF Research Database (Denmark)

    Petersen, Karen Bjerg; Andersen, Frans Ørsted

    2018-01-01

    artikel introduceres og diskuteres en succesrig ny ungdomsuddannelse, Pathway to Technology (P-tech), som i fælles bestræbelser mellem forskellige uddannelsesinstitutioner, virksomheder og politiske tiltag retter sig specifikt mod de store marginaliserede og udsatte ungdomsrestgrupper i USA. I Danmark...

  8. 78 FR 71546 - Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY

    Science.gov (United States)

    2013-11-29

    ... 1625-AA00 Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY AGENCY: Coast... the construction of the Bridge. DATES: Comments and related material must be received by the Coast... construction and demolition of Belt Parkway Bridge. There is no previous regulatory action for this Bridge. C...

  9. 76 FR 20530 - Safety Zone; Boom Days, Buffalo Outer Harbor, Buffalo, NY

    Science.gov (United States)

    2011-04-13

    ...[deg]51'47.61'' W (NAD 83). (b) Effective period. This regulation will be effective and the safety zone...-AA00 Safety Zone; Boom Days, Buffalo Outer Harbor, Buffalo, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone in the Buffalo Outer...

  10. 75 FR 42608 - Safety Zone; Lyme Community Days, Chaumont Bay, NY

    Science.gov (United States)

    2010-07-22

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Lyme Community Days, Chaumont Bay, NY... temporary safety zone for Lyme Community Days Fireworks on Chaumont Bay, Lyme, New York. All vessels are... vessels during the setup, loading, and launching of a fireworks display in conjunction with the Lyme...

  11. 78 FR 50102 - Notice of Inventory Completion: Rochester Museum & Science Center, Rochester, NY

    Science.gov (United States)

    2013-08-16

    .... 3003, of the completion of an inventory of associated funerary objects under the control of the....R50000] Notice of Inventory Completion: Rochester Museum & Science Center, Rochester, NY AGENCY: National... inventory of associated funerary objects, in consultation with the appropriate Indian tribes or Native...

  12. 77 FR 33309 - Safety Zone; Race on the Lake, Onondaga Lake, Syracuse, NY

    Science.gov (United States)

    2012-06-06

    ...-AA00 Safety Zone; Race on the Lake, Onondaga Lake, Syracuse, NY AGENCY: Coast Guard, DHS. ACTION... Race on the Lake powerboat races. This temporary safety zone is necessary to protect participants, spectators, and vessels from the hazards associated with hydroplane and powerboat races. DATES: This rule is...

  13. 75 FR 7030 - Dawson Metal Company, Inc., Industrial Division, Jamestown, NY; Notice of Affirmative...

    Science.gov (United States)

    2010-02-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Dawson Metal Company, Inc., Industrial Division, Jamestown, NY; Notice... determination based on the finding that imports of precision sheet metal fabrication did not contribute...

  14. 77 FR 19698 - Notice of Intent to Repatriate Cultural Items: Rochester Museum & Science Center, Rochester, NY

    Science.gov (United States)

    2012-04-02

    ... Trading Post, in Collins, NY. Traditional religious leaders of the Seneca Nation of New York have... religious leaders for the practice of traditional Native American religions by their present adherents, and... relationship of shared group identity that can be reasonably traced between these medicine faces and the Seneca...

  15. 77 FR 42508 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2012-07-19

    ... Inventory Completion: New York University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The New York University College of Dentistry has completed an inventory... the New York University College of Dentistry. Repatriation of the human remains to the Indian tribes...

  16. 75 FR 15739 - International Business Machines Corporation: Armonk, NY; Notice of Termination of Investigation

    Science.gov (United States)

    2010-03-30

    ... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-70,580] International Business Machines Corporation: Armonk, NY; Notice of Termination of Investigation Pursuant to Section 223 of the..., 2009 on behalf of workers of International Business Machines Corporation, Armonk, New York. The...

  17. 77 FR 58439 - Environmental Impact Statement for the Northeast Corridor Between Washington, DC, New York, NY...

    Science.gov (United States)

    2012-09-20

    ...] Environmental Impact Statement for the Northeast Corridor Between Washington, DC, New York, NY and Boston, MA.... The Northeast faces major congestion and capacity constraints that, if not addressed, will have the.... Rebecca Pennington, Chief Financial Officer, Federal Railroad Administration. [FR Doc. 2012-23232 Filed 9...

  18. Modelling dune erosion, overwash and breaching at Fire Island (NY) during hurricane Sandy

    NARCIS (Netherlands)

    De Vet, P.L.M.; McCall, R.T.; Den Bieman, J.P.; Stive, M.J.F.; Van Ormondt, M.

    2015-01-01

    In 2012, Hurricane Sandy caused a breach at Fire Island (NY, USA), near Pelican Island. This paper aims at modelling dune erosion, overwash and breaching processes that occured during the hurricane event at this stretch of coast with the numerical model XBeach. By using the default settings, the

  19. 77 FR 74866 - Notice of Intent To Repatriate Cultural Items: New York State Museum, Albany, NY

    Science.gov (United States)

    2012-12-18

    ... State Museum, 3122 Cultural Education Center, Albany, NY 12230, telephone (518) 486- 2020. SUPPLEMENTARY... reported the belt was exchanged during the wars between the Huron and Seneca Nations. According to Chief.... Converse reported the Ely S. Parker belt was a war belt and a national belt of the Seneca people. The belt...

  20. 76 FR 4078 - Television Broadcasting Services; North Pole and Plattsburgh, NY

    Science.gov (United States)

    2011-01-24

    ... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [DA 10-2443; MM Docket No. 99-238; RM-9669] Television Broadcasting Services; North Pole and Plattsburgh, NY AGENCY: Federal Communications Commission... CFR Part 73 Television, Television broadcasting. For the reasons discussed in the preamble, the...

  1. 77 FR 52058 - Notice of Inventory Completion: Longyear Museum of Anthropology, Colgate University, Hamilton, NY

    Science.gov (United States)

    2012-08-28

    ... Inventory Completion: Longyear Museum of Anthropology, Colgate University, Hamilton, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Longyear Museum of Anthropology has completed an... cultural affiliation with the human remains should contact the Longyear Museum of Anthropology at the...

  2. 76 FR 48178 - Notice of Inventory Completion: Longyear Museum of Anthropology, Colgate University, Hamilton, NY

    Science.gov (United States)

    2011-08-08

    ...: Longyear Museum of Anthropology, Colgate University, Hamilton, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Longyear Museum of Anthropology has completed an inventory of a human remain... human remain should contact the Longyear Museum of Anthropology at the address below by September 7...

  3. 77 FR 52058 - Notice of Inventory Completion: American Museum of Natural History, New York, NY; Correction

    Science.gov (United States)

    2012-08-28

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY... completion of an inventory of human remains in the possession of the American Museum of Natural History, New... following sentence: Officials of the American Museum of Natural History have determined that, pursuant to 25...

  4. 76 FR 43712 - Notice of Inventory Completion: American Museum of Natural History, New York, NY

    Science.gov (United States)

    2011-07-21

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The American Museum of Natural History... human remains may contact the American Museum of Natural History. Repatriation of the human remains to...

  5. 77 FR 42640 - Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY

    Science.gov (United States)

    2012-07-20

    ...-AA00 Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY AGENCY: Coast Guard... Black River Bay during the Can-Am Festival Fireworks display. This temporary safety zone is necessary to... Can-Am Festival Fireworks. This zone will be effective and enforced from 9:15 p.m. until 10:45 p.m. on...

  6. 77 FR 39638 - Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY

    Science.gov (United States)

    2012-07-05

    ...-AA00 Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY AGENCY: Coast Guard, DHS... during the Barbara Harder Wedding Fireworks. This temporary safety zone is necessary to protect... vessels during the Barbara Harder Wedding Fireworks. This zone will be effective and enforced from 9 p.m...

  7. 77 FR 46349 - Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY

    Science.gov (United States)

    2012-08-03

    ... 1625-AA00 Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY AGENCY: Coast... wedding fireworks display. This action is necessary to provide for the safety of life on navigable waters... DeStefano family wedding fireworks display. This proposed regulated area includes all waters of Patchogue...

  8. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Science.gov (United States)

    Milne, Katy; Barnes, Rebecca O; Girardin, Adam; Mawer, Melanie A; Nesslinger, Nancy J; Ng, Alvin; Nielsen, Julie S; Sahota, Robert; Tran, Eric; Webb, John R; Wong, May Q; Wick, Darin A; Wray, Andrew; McMurtrie, Elissa; Köbel, Martin; Kalloger, Steven E; Gilks, C Blake; Watson, Peter H; Nelson, Brad H

    2008-01-01

    Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC). A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B) and other immunological markers (CD20, MHC class I and MHC class II). Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35) of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004). Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant T cell antigens for immunotherapy.

  9. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Katy Milne

    Full Text Available BACKGROUND: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC. A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. METHODOLOGY AND PRINCIPAL FINDINGS: We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B and other immunological markers (CD20, MHC class I and MHC class II. Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35 of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004. Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. CONCLUSION AND SIGNIFICANCE: We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant

  10. Effect of functional yogurt NY-YP901 in improving the trait of metabolic syndrome.

    Science.gov (United States)

    Chang, B J; Park, S U; Jang, Y S; Ko, S H; Joo, N M; Kim, S I; Kim, C-H; Chang, D K

    2011-11-01

    This study was aimed to assess the beneficial effects on metabolic syndrome of functional yogurt NY-YP901 (Namyang Dairy Product Co. Ltd and Nutra R&BT Inc., Seoul, Korea) supplemented with mixture of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium infantis and extra-ingredients containing Bifidobacterium breve (CBG-C2), Enterococcus faecalis FK-23, fibersol-2 and so on. This study was designed as an 8-week randomized, double-blind, placebo-controlled, parallel study. Treatment and control groups consumed a functional yogurt NY-YP901 (150 ml) and a placebo yogurt twice a day, respectively, for 8 weeks. Body weight and body mass index (BMI), blood pressure, lipid profiles, fasting glucose with HbA1C and waist circumference were measured before and after treatment. Inclusion criteria were healthy individuals between the ages 20-65 years old who submitted an informed consent. During the period August 2009 to December 2009, 101 healthy participants (31 males and 70 females) finished the study. Treatment group were 53 individuals, and the control group were 48 individuals. In the treatment group consuming NY-YP901, statistically significant beneficial changes were observed in body weight (treatment group vs control group=-0.24±1.50 vs +0.64±1.39 kg, Pcholesterol (-7.71±14.14 vs -0.43±15.32 mg/dl, Pyogurt NY-YP901 reduced LDL-cholesterol, body weight and BMI in the subjects at a 300-ml consumption daily for 8 weeks. From these findings, regular intake of functional yogurt NY-YP901 may be consequently related to improve metabolic syndrome.

  11. Allegheny County School District Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates the school district boundaries within Allegheny County If viewing this description on the Western Pennsylvania Regional Data Center’s open...

  12. Allegheny County Park Rangers Outreach

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Launched in June 2015, the Allegheny County Park Rangers program reached over 48,000 people in its first year. Park Rangers interact with residents of all ages and...

  13. Allegheny County Poor Housing Conditions

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This estimate of the percent of distressed housing units in each Census Tract was prepared using data from the American Community Survey and the Allegheny County...

  14. Allegheny County Public Building Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains the locations of municipal facilities in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open...

  15. Allegheny County Primary Care Access

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The data on health care facilities includes the name and location of all the hospitals and primary care facilities in Allegheny County. The current listing of...

  16. Allegheny County Addressing Street Centerlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the road centerlines in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  17. Allegheny County Addressing Segment Aliases

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This table contains the segment aliases for roads in Allegheny County that may have an alternate street nameIf viewing this description on the Western Pennsylvania...

  18. Allegheny County Addressing Street Aliases

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the road centerlines in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  19. Allegheny County Jail Daily Census

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — A daily census of the inmates at the Allegheny County Jail (ACJ). Includes gender, race, age at booking, and current age. The records for each month contain a...

  20. 2015 Lowndes County (GA) Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — TASK NAME: NOAA OCM Lidar for Lowndes County, GA with the option to Collect Lidar in Cook and Tift Counties, GA Lidar Data Acquisition and Processing Production Task...

  1. Curry County E-911 Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Line attributes denoting all street centerlines in Curry County. Dataset includes all centerlines for all county maintained roads, all state and federal highways,and...

  2. TERRAIN, PROVIDENCE COUNTY, RHODE ISLAND

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Providence AOI consists of the costal portion of the county, and meshes up seamlessly with the Kent county AOI directly south. Ground Control is collected...

  3. Allegheny County Summer Food Sites

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This data set shows the Summer Food Sites located within Allegheny County for children (18 years and younger) for breakfast and lunch during summer recess. OPEN...

  4. Allegheny County Commercial Vehicle Inspections

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset lists the locations and results of all commercial vehicle inspections performed by the Allegheny County Police Motor Carrier Safety Assistance Program...

  5. Providing engineering services to counties.

    Science.gov (United States)

    2008-09-01

    An engineer is required by law to safeguard the health, safety and welfare of the public. The current Kansas : statute state, The Board of County Commissioners of each county shall appoint a licensed professional : engineer, whose title shall be c...

  6. Allegheny County Mortgage Foreclosure Records

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This data includes filings related to mortgage foreclosure in Allegheny County. The foreclosure process enables a lender to take possession of a property due to an...

  7. Grant County E-911 Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This data set contains a vector digital representation of all accessible roads in the county including interstate highways, State highways, county roads and city...

  8. Allegheny County Property Sale Transactions

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains data on all Real Property parcels that have sold since 2013 in Allegheny County, PA. Before doing any market analysis on property sales, check...

  9. Allegheny County Fatal Accidental Overdoses

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Fatal accidental overdose incidents in Allegheny County, denoting age, gender, race, drugs present, zip code of incident and zip code of residence. Zip code of...

  10. Allegheny County Cell Tower Points

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset portrays cell tower locations as points in Allegheny County. The dataset is based on outbuilding codes in the Property Assessment Parcel Database used...

  11. Allegheny County Zip Code Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates the zip code boundaries that lie within Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open...

  12. Sonoma County, CA, 2013 Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sonoma County Vegetation Mapping and LiDAR Consortium retained WSI to provide lidar and Orthophoto data and derived products in Sonoma County, CA. A classified LAS...

  13. Valencia County E-911 Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This data set contains a vector digital representation of all accessible roads in the county including interstate highways, State highways, county roads and some...

  14. Allegheny County Fast Food Establishments

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Allegheny County Health Department has generated this list of fast food restaurants by exporting all chain restaurants without an alcohol permit from the...

  15. Allegheny County Land Use Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Allegheny County land use as ascribed to areas of land. The Land Use Feature Dataset contains photogrammetrically compiled information concerning vegetation and...

  16. Allegheny County Employee Salaries 2016

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Employee salaries are a regular Right to Know request the County receives. Here is the disclaimer language that is included with the dataset from the Open Records...

  17. Polymeric structure and host Toll-like receptor 4 dictate immunogenicity of NY-ESO-1 antigen in vivo.

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W; Aldridge, Michael E; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-10-28

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern.

  18. Polymeric Structure and Host Toll-like Receptor 4 Dictate Immunogenicity of NY-ESO-1 Antigen in Vivo*

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W.; Aldridge, Michael E.; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P.; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-01-01

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern. PMID:21900253

  19. Minnesota County Boundaries

    Data.gov (United States)

    Minnesota Department of Natural Resources — Minnesota county boundaries derived from a combination of 1:24,000 scale PLS lines, 1:100,000 scale TIGER, 1:100,000 scale DLG, and 1:24,000 scale hydrography lines....

  20. Minnesota County Boundaries - lines

    Data.gov (United States)

    Minnesota Department of Natural Resources — Minnesota county boundaries derived from a combination of 1:24,000 scale PLS lines, 1:100,000 scale TIGER, 1:100,000 scale DLG, and 1:24,000 scale hydrography lines....

  1. Oncolytic viruses sensitize human tumor cells for NY-ESO-1 tumor antigen recognition by CD4+ effector T cells.

    Science.gov (United States)

    Delaunay, Tiphaine; Violland, Mathilde; Boisgerault, Nicolas; Dutoit, Soizic; Vignard, Virginie; Münz, Christian; Gannage, Monique; Dréno, Brigitte; Vaivode, Kristine; Pjanova, Dace; Labarrière, Nathalie; Wang, Yaohe; Chiocca, E Antonio; Boeuf, Fabrice Le; Bell, John C; Erbs, Philippe; Tangy, Frédéric; Grégoire, Marc; Fonteneau, Jean-François

    2018-01-01

    Oncolytic immunotherapy using oncolytic viruses (OV) has been shown to stimulate the antitumor immune response by inducing the release of tumor-associated antigens (TAA) and danger signals from the dying infected tumor cells. In this study, we sought to determine if the lysis of tumor cells induced by different OV: measles virus, vaccinia virus, vesicular stomatitis virus, herpes simplex type I virus, adenovirus or enterovirus, has consequences on the capacity of tumor cells to present TAA, such as NY-ESO-1. We show that the co-culture of NY-ESO-1 neg /HLA-DP4 pos melanoma cells with NY-ESO-1 pos /HLA-DP4 neg melanoma cells infected and killed by different OV induces an intercellular transfer of NY-ESO-1 that allows the recognition of NY-ESO-1 neg /HLA-DP4 pos tumor cells by an HLA-DP4/NY-ESO-1 (157-170) -specific CD4+ cytotoxic T cell clone, NY67. We then confirmed this result in a second model with an HLA-DP4+ melanoma cell line that expresses a low amount of NY-ESO-1. Recognition of this cell line by the NY67 clone is largely increased in the presence of OV productive infection. Altogether, our results show for the first time another mechanism of stimulation of the anti-tumor immune response by OV, via the loading of tumor cells with TAA that sensitizes them for direct recognition by specific effector CD4+ T cells, supporting the use of OV for cancer immunotherapy.

  2. Effector memory and central memory NY-ESO-1-specific re-directed T cells for treatment of multiple myeloma.

    Science.gov (United States)

    Schuberth, P C; Jakka, G; Jensen, S M; Wadle, A; Gautschi, F; Haley, D; Haile, S; Mischo, A; Held, G; Thiel, M; Tinguely, M; Bifulco, C B; Fox, B A; Renner, C; Petrausch, U

    2013-04-01

    The cancer-testis antigen NY-ESO-1 is a potential target antigen for immune therapy expressed in a subset of patients with multiple myeloma. We generated chimeric antigen receptors (CARs) recognizing the immunodominant NY-ESO-1 peptide 157-165 in the context of HLA-A*02:01 to re-direct autologous CD8(+) T cells towards NY-ESO-1(+) myeloma cells. These re-directed T cells specifically lysed NY-ESO-1(157-165)/HLA-A*02:01-positive cells and secreted IFNγ. A total of 40% of CCR7(-) re-directed T cells had an effector memory phenotype and 5% a central memory phenotype. Based on CCR7 cell sorting, effector and memory CAR-positive T cells were separated and CCR7(+) memory cells demonstrated after antigen-specific re-stimulation downregulation of CCR7 as sign of differentiation towards effector cells accompanied by an increased secretion of memory signature cytokines such as IL-2. To evaluate NY-ESO-1 as potential target antigen, we screened 78 bone marrow biopsies of multiple myeloma patients where NY-ESO-1 protein was found to be expressed by immunohistochemistry in 9.7% of samples. Adoptively transferred NY-ESO-1-specific re-directed T cells protected mice against challenge with endogenously NY-ESO-1-positive myeloma cells in a xenograft model. In conclusion, re-directed effector- and central memory T cells specifically recognized NY-ESO-1(157-165)/ HLA-A*02:01-positive cells resulting in antigen-specific functionality in vitro and in vivo.

  3. Enhancing whole-tumor cell vaccination by engaging innate immune system through NY-ESO-1/dendritic cell interactions.

    Science.gov (United States)

    Xu, Le; Zheng, Junying; Nguyen, David H; Luong, Quang T; Zeng, Gang

    2013-10-01

    NY-ESO-1 is a cancer/germline antigen (Ag) with distinctively strong immunogenicity. We have previously demonstrated that NY-ESO-1 serves as an endogenous adjuvant by engaging dendritic cell (DC)-surface receptors of calreticulin (CRT) and toll-like receptor (TLR) 4. In the present study, NY-ESO-1 was investigated for its immunomodulatory roles as a molecular adjuvant in whole-tumor cell vaccines using the Renca kidney cancer model. Renca cells were genetically engineered to express NY-ESO-1 on the cell surface to enhance direct interactions with DC. The effect of ectopic cell-surface expression of NY-ESO-1 was investigated on tumor immunogenicity, DC activation, cytotoxic T lymphocytes against model tumor-associated Ags, and the effectiveness of the modified tumor cells as a therapeutic whole-cell vaccine. Cell-surface expression of NY-ESO-1 was able to reduce the tumor growth of Renca cells in BALB/c mice, although the modification did not alter cell proliferation rate in vitro. Directly engaging the innate immune system through NY-ESO-1 facilitated the interaction of tumor cells with DC, leading to enhanced DC activation and subsequent tumor-specific T-cell priming. When used as a therapeutic whole-cell vaccine, Renca cells with NY-ESO-1 on the surface mediated stronger inhibitory effects on tumor growth and metastasis compared with parental Renca or Renca cells expressing a control protein GFP on the surface. Augmented antitumor efficacy correlated with increased CD8 T-cell infiltration into tumors and decreased myeloid-derived suppressor cells and regulatory T cells in the spleen. As a cancer/germline Ag and as an immunomodulatory adjuvant through engaging innate immune receptors, NY-ESO-1 offers a unique opportunity for improved whole-tumor cell vaccinations upon the classic GM-CSF-engineered cell vaccines.

  4. NY-ESO-1 is a ubiquitous immunotherapeutic target antigen for patients with myxoid/round cell liposarcoma.

    Science.gov (United States)

    Pollack, Seth M; Jungbluth, Achim A; Hoch, Benjamin L; Farrar, Erik A; Bleakley, Marie; Schneider, David J; Loggers, Elizabeth T; Rodler, Eve; Eary, Janet F; Conrad, Ernest U; Jones, Robin L; Yee, Cassian

    2012-09-15

    Myxoid/round cell liposarcoma (MRCL) is the second most common liposarcoma subtype, accounting for >33% of liposarcomas and approximately 10% of all soft tissue sarcomas. Although MRCL is a chemosensitive subtype, patients with metastatic disease have a poor outcome. NY-ESO-1 is a cancer-testis antigen (also known as cancer germ cell antigen) that has been successfully targeted in vaccine trials and in adoptive T-cell therapy trials for the treatment of several solid tumors. The authors investigated the feasibility of targeting NY-ESO-1 in patients with MRCL by evaluating the prevalence of NY-ESO-1 expression in tumors using immunohistochemistry and quantitative reverse transcriptase-polymerase chain reaction analysis. NY-ESO-1-specific tumor recognition by NY-ESO-1-specific T-cells also was analyzed using a chromium release assay. A search of the University of Washington Sarcoma Tissue Bank identified paraffin-embedded tumor samples from 25 patients with MRCL. NY-ESO-1 expression was observed in every MRCL tumor assessed (100%); in 18 tumors (72%), staining was homogenous. In all but 2 tumors, staining was sufficiently robust (2+) that such patients would be eligible for clinical trials of NY-ESO-1-directed therapy. By using NY-ESO-1 specific, CD8-positive T-cells, the in vitro sensitivity of myxoid liposarcoma cell lines to antigen-specific lysis was demonstrated. The current results establish NY-ESO-1 as an important target antigen for the treatment of patients with MRCL. Copyright © 2012 American Cancer Society.

  5. MAGE-A3/4 and NY-ESO-1 antigens expression in metastatic esophageal squamous cell carcinoma.

    Science.gov (United States)

    Bujas, T; Marusic, Z; Peric Balja, M; Mijic, A; Kruslin, B; Tomas, D

    2011-03-21

    In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.021) but no significant correlation with the expression of MAGE-A 3/4 in lymph node metastases (P=0.056). Expression of NY-ESO-1 in primary tumors showed significant positive correlation with the expression of NY-ESO-1 in lymph node metastases (P=0.001) and significant negative correlation with patients’ age (P<0.001). Expression of MAGE-A 3/4 and NY-ESO-1 in primary tumors and lymph node metastases showed no significant correlation with prognostic parameters such as tumor grade and TNM stage (P>0.05). We have shown different levels of MAGE-A 3/4 and NY-ESO-1 expression in almost all specimens of primary tumor and lymph node metastases

  6. NY-ESO-1 Protein Cancer Vaccine With Poly-ICLC and OK-432: Rapid and Strong Induction of NY-ESO-1-specific Immune Responses by Poly-ICLC.

    Science.gov (United States)

    Takeoka, Tomohira; Nagase, Hirotsugu; Kurose, Koji; Ohue, Yoshihiro; Yamasaki, Makoto; Takiguchi, Shuji; Sato, Eiichi; Isobe, Midori; Kanazawa, Takayuki; Matsumoto, Mitsunobu; Iwahori, Kota; Kawashima, Atsunari; Morimoto-Okazawa, Akiko; Nishikawa, Hiroyoshi; Oka, Mikio; Pan, Linda; Venhaus, Ralph; Nakayama, Eiichi; Mori, Masaki; Doki, Yuichiro; Wada, Hisashi

    2017-03-23

    We conducted a clinical trial of a cancer vaccine using NY-ESO-1 protein with polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) and/or OK-432 against solid tumors. A total of 15 patients were sequentially enrolled in 4 cohorts. Patients in cohort 1 received NY-ESO-1 protein; cohort 2a received NY-ESO-1 protein+OK-432; cohort 2b received NY-ESO-1 protein+poly-ICLC; cohort 3 received NY-ESO-1 protein+OK-432+poly-ICLC with Montanide ISA-51. The endpoints of this trial were safety, NY-ESO-1 immune responses, and clinical response. Vaccine-related adverse events observed were fever and injection-site reaction (grade 1). Two patients showed stable disease after vaccination. NY-ESO-1 antibodies were observed in 4 patients at the baseline (sero-positive) and augmented in all patients after vaccination. Eleven patients showed a conversion of negative antibody responses at baseline to positive after vaccination (seroconversion). The seroconversions were observed in all 11 sero-negative patients by the fourth immunization; in particular, it was observed by the second immunization in patients with poly-ICLC, and these induced antibody responses were stronger than those in patients immunized without poly-ICLC. The number of NY-ESO-1-specific interferon (IFN)γ-producing T cells was increased in patients immunized with poly-ICLC and/or OK-432, and furthermore, the increase of IFNγ-producing CD8 T cells in patients immunized with poly-ICLC was significantly higher than that in patients without poly-ICLC. Nonspecific activations of T-cell or antigen presenting cells were not observed. Our present study showed that poly-ICLC is a promising adjuvant for cancer vaccines.

  7. Ny krigsførelse i informationsalderen? Om ‘Revolution in Military Affairs’ og Irakkrigen

    Directory of Open Access Journals (Sweden)

    Jens Claus Hansen

    2005-09-01

    Full Text Available Artiklen diskuterer, hvorvidt man efter Golfkrigen kan tale om en ‘Revolu- tion in Military Affairs’ – RMA – som nogle amerikanske krigsteoretikere hævder har fundet sted med anvendelsen af ny teknologi i moderne krigs- førelse. Forfatteren viser, hvordan moderne krigsstrategier har inkorpore- ret ny teknologi og mediebrug som bl.a. informationskrig, ‘Systems of Sys- tems’ og Network Centric Warfare. Forfatteren påpeger, at den strategiske anvendelse af informationsoperationer – herunder ‘embedded’ journalister – ikke har medført en demokratisering af nyhedsdækningen og heller ikke indebærer en øget sandhedsværdi. Afslutningsvis peger forfatteren på behovet for en ‘Revolution in Diplomatic Affairs’.

  8. Ny kandidatuddannelse i Bæredygtig Energi på Risø DTU

    DEFF Research Database (Denmark)

    Ryde, M.

    2007-01-01

    I september 2008 vil de første studerende begynde på en ny kandidatuddannelse med basis på Risø DTU. Det samlede studieforløb er normeret til to år, og de studerende er således godt i gang med deres studium når FN’s klimakonference afholdes i København2009...

  9. Agil Stage-Gate®: ny model for udviklingsprojekter i mellemstore virksomheder

    DEFF Research Database (Denmark)

    Vedsmand, Tomas; Edwards, Kasper; Hvidt, Nicoline

    Lær at udvikle nye produkter hurtigere og med større træfsikkerhed – ved at arbejde i sprint med agile metoder og træffe de rigtige beslutninger med Stage-Gate® Se også tilhørende: "Værktøjskasse til Agil Stage-Gate®: Ny model for udviklingsprojekter i mellemstore virksomheder" på http://dx.doi.org...

  10. Immunohistochemical assessment of NY-ESO-1 expression in esophageal adenocarcinoma resection specimens.

    Science.gov (United States)

    Hayes, Stephen J; Hng, Keng Ngee; Clark, Peter; Thistlethwaite, Fiona; Hawkins, Robert E; Ang, Yeng

    2014-04-14

    To assess NY-ESO-1 expression in a cohort of esophageal adenocarcinomas. A retrospective search of our tissue archive for esophageal resection specimens containing esophageal adenocarcinoma was performed, for cases which had previously been reported for diagnostic purposes, using the systematised nomenclature of human and veterinary medicine coding system. Original haematoxylin and eosin stained sections were reviewed, using light microscopy, to confirm classification and tumour differentiation. A total of 27 adenocarcinoma resection specimens were then assessed using immunohistochemistry for NY-ESO-1 expression: 4 well differentiated, 14 moderately differentiated, 4 moderate-poorly differentiated, and 5 poorly differentiated. Four out of a total of 27 cases of esophageal adenocarcinoma examined (15%) displayed diffuse cytoplasmic and nuclear expression for NY-ESO-1. They displayed a heterogeneous and mosaic-type pattern of diffuse staining. Diffuse cytoplasmic staining was not identified in any of these structures: stroma, normal squamous epithelium, normal submucosal gland and duct, Barrett's esophagus (goblet cell), Barrett's esophagus (non-goblet cell) and high grade glandular dysplasia. All adenocarcinomas showed an unexpected dot-type pattern of staining at nuclear, paranuclear and cytoplasmic locations. Similar dot-type staining, with varying frequency and size of dots, was observed on examination of Barrett's metaplasia, esophageal submucosal gland acini and the large bowel negative control, predominantly at the crypt base. Furthermore, a prominent pattern of apical (luminal) cytoplasmic dot-type staining was observed in some cases of Barrett's metaplasia and also adenocarcinoma. A further morphological finding of interest was noted on examination of haematoxylin and eosin stained sections, as aggregates of lymphocytes were consistently noted to surround submucosal glands. We have demonstrated for the first time NY-ESO-1 expression by esophageal

  11. Udvikling af en internetsøgestrategi til identifikation af ny medicinsk teknologi inden for prostatacancer

    DEFF Research Database (Denmark)

    Thygesen, Marianne; Vondeling, Hindrik; Douw, Karla

    2007-01-01

    internetsider efter efficiens. Her er efficiens forstået som mindst antal minutter brugt pr. fund af information om en ny væsentlig medicinsk teknologi. Resultater: Strategien består af ti sider, som bør benyttes i følgende rækkefølge: YahooHealth, DoctorsGuide, The National Electronic Library for Medicines...

  12. InSync Adaptive Traffic Control System for the Veterans Memorial Hwy Corridor on Long Island, NY

    Science.gov (United States)

    2012-08-01

    This report documents Rhythm Engineerings adaptive traffic control system field installation performed : by New York State Department of Transportation (NYSDOT) along Veterans Memorial Hwy in Long : Island, NY. This report reviews the reason for t...

  13. Limerick, City and County

    OpenAIRE

    2011-01-01

    Postcard. Colour drawings of maps of Limerick city and county and Foynes - transatlantic air base flying boat, Dromore Castle, Glenstal Abbey, Ardagh Chalice, Askeaton; the Abbey, Gate Loge Adare Manor, Newcastlewest, King John's Castle, St. Mary's Cathedral (Church of Ireland), The Old Custom House, The Hunt Museum, The Old Mill and Bridge croom, The Coll (de Valera) Cottage Buree, Town Gate Kilmallock, Lough Gur Interpretive Centre, Hospital Ancient hostelry and The Treaty Stone. Copyright ...

  14. Decadal Climate Change in Ny-Ålesund, Svalbard, A Representative Area of the Arctic

    Directory of Open Access Journals (Sweden)

    Minghu Ding

    2018-04-01

    Full Text Available In recent decades, global warming hiatus/slowdown has attracted considerable attention and has been strongly debated. Many studies suggested that the Arctic is undergoing rapid warming and significantly contributes to a continual global warming trend rather than a hiatus. In this study, we evaluated the climate changes of Ny-Ålesund, Svalbard, a representative location of the northern North Atlantic sector of the Arctic, based on observational records from 1975–2014. The results showed that the annual warming rate was four times higher than the global mean (+0.76 °C·decade−1 and was also much greater than Arctic average. Additionally, the warming trend of Ny-Ålesund started to slow down since 2005–2006, and our estimates showed that there is a 8–9 years-lagged, but significant, correlation between records of Ny-Ålesund and global HadCRUT4 datasets. This finding indicates that the Arctic was likely experiencing a hiatus pattern, which just appeared later than the low-mid latitudes due to transport processes of atmospheric circulations and ocean currents, heat storage effect of cryospheric components, multidecadal variability of Arctic cyclone activities, etc. This case study provides a new perspective on the global warming hiatus/slowdown debate.

  15. Distributions and impact factors of antimony in topsoils and moss in Ny-Ålesund, Arctic

    International Nuclear Information System (INIS)

    Jia Nan; Sun Liguang; He Xin; You Kehua; Zhou Xin; Long Nanye

    2012-01-01

    The distribution of antimony (Sb) in topsoil and moss (Dicranum angustum) in disturbed and undisturbed areas, as well as coal and gangue, in Ny-Ålesund, Arctic was examined. Results show that the weathering of coal bed could not contribute to the increase of Sb concentrations in topsoil and moss in the study area. The distribution of Sb is partially associated with traffic and historical mining activities. The occurrence of the maximum Sb concentration is due to the contribution of human activities. In addition, the decrease of Sb content in topsoil near the coastline may be caused by the washing of seawater. Compared with topsoils, moss could be a useful tool for monitoring Sb in both highly and lightly polluted areas. - Highlights: ► We examined the distribution of antimony in topsoil and moss in Ny-Ålesund, Arctic. ► The distribution of Sb is associated with traffic and historical mining activities. ► The weathering of coal bed cannot lead to the Sb increase in the surface environment. ► The distribution of Sb in moss could reflect the accumulation of Sb pollution. ► Moss can be a useful tool for monitoring Sb in highly and lightly polluted areas. - The distribution of Sb in Ny-Ålesund is partially associated with traffic and historical mining activities, and moss can be a useful tool for monitoring Sb in both highly and lightly polluted areas.

  16. Research Article. A new gravity laboratory in Ny-Ålesund, Svalbard

    Science.gov (United States)

    Breili, K.; Hougen, R.; Lysaker, D. I.; Omang, O. C. D.; Tangen, B.

    2017-02-01

    The Norwegian Mapping Authority (NMA) has recently established a new gravity laboratory in Ny-Ålesund at Svalbard, Norway. The laboratory consists of three independent pillars and is part of the geodetic core station that is presently under construction at Brandal, approximately 1.5 km north of NMA's old station. In anticipation of future use of the new gravity laboratory, we present benchmark gravity values, gravity gradients, and final coordinates of all new pillars. Test measurements indicate a higher noise level at Brandal compared to the old station. The increased noise level is attributed to higher sensitivity to wind.We have also investigated possible consequences of moving to Brandal when it comes to the gravitational signal of present-day ice mass changes and ocean tide loading. Plausible models representing ice mass changes at the Svalbard archipelago indicate that the gravitational signal at Brandal may differ from that at the old site with a size detectable with modern gravimeters. Users of gravity data from Ny-Ålesund should, therefore, be cautious if future observations from the new observatory are used to extend the existing gravity record. Due to its lower elevation, Brandal is significantly less sensitive to gravitational ocean tide loading. In the future, Brandal will be the prime site for gravimetry in Ny-Ålesund. This ensures gravity measurements collocated with space geodetic techniques like VLBI, SLR, and GNSS.

  17. UNEMPLOYMENT IN HUNEDOARA COUNTY

    Directory of Open Access Journals (Sweden)

    CLAUDIA ISAC

    2015-12-01

    Full Text Available Unemployment highlights a state of imbalance on the labour market which is characterized by a surplus of workforce in relation to job vacancies. This imbalance has been more apparent in Hunedoara County than in other counties, due to the fact that there are 3 mono-industrial areas that have been restructured over the past two decades. The effects are presented in this paper in the form of a complex statistical analysis. Thus, based on the evolution of the number of unemployed individuals in 1995, one can observe the periods of significant adverse effects upon the degree of employment. Moreover, one can make correlations with periods of international financial crisis and with the number of employees in the County in order to determine significant variables of the unemployment phenomenon. The content of this paper is significant and represents the analysis of the number of unemployed in the Jiu Valley, scattered across towns. As a form of financial protection, the unemployment benefit represents a financial instrument in the cases determined by this negative phenomenon, which is why in conclusion we make a comparison of the ways this aid is granted throughout several years and in various forms.

  18. Værktøjskasse til Agil Stage-Gate®: Ny model for udviklingsprojekter i mellemstore virksomheder

    DEFF Research Database (Denmark)

    Vedsmand, Tomas; Edwards, Kasper; Hvidt, Nicoline

    Denne værktøjskasse er et tillæg for guiden Agil Stage-Gate: Ny model for udviklingsprojekter i mellemstore virksomheder. (http://dx.doi.org/10.11581/DTU:00000028)......Denne værktøjskasse er et tillæg for guiden Agil Stage-Gate: Ny model for udviklingsprojekter i mellemstore virksomheder. (http://dx.doi.org/10.11581/DTU:00000028)...

  19. Resiquimod as an Immunologic Adjuvant for NY-ESO-1 Protein Vaccination in Patients with High Risk Melanoma

    Science.gov (United States)

    Gnjatic, Sacha; Cruz, Crystal M.; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P.; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A.; Bhardwaj, Nina

    2015-01-01

    The TLR7/8 agonist, Resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide with or without Resiquimod in high-risk melanoma patients. In Part I of the study, patients received 100ug full length NY-ESO-1 protein emulsified in 1.25mL Montanide (day 1) followed by topical application of 1000mg of 0.2% Resiquimod gel on days 1 and 3 (Cohort 1) versus days 1, 3, and 5 (Cohort 2) of a 21 day cycle. In Part II, patients were randomized to receive 100ug NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel (Arm-A; N=8) or 1000mg of 0.2% Resiquimod gel (Arm-B; N=12) using the dosing regimen established in Part I. The vaccine regimens were generally well-tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In Part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4+ T-cell responses. CD8+ T-cell responses were only seen in 3 of 12 patients in Arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8+ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical Resiquimod is safe and induces both antibody and CD4+ T-cell responses in the majority of patients; the small proportion of CD8+ T-cell responses suggests that the addition of topical Resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8+ T-cell responses. PMID:25633712

  20. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma.

    Directory of Open Access Journals (Sweden)

    Lisa M Ebert

    Full Text Available Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg, which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIX™ cancer vaccine. All patients tested had Treg (CD25(bright FoxP3(+ CD127(neg specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1(157-170 epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1(115-132 peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials.

  1. NY-ESO-1- and survivin-specific T-cell responses in the peripheral blood from patients with glioma.

    Science.gov (United States)

    Liu, Zhenjiang; Poiret, Thomas; Persson, Oscar; Meng, Qingda; Rane, Lalit; Bartek, Jiri; Karbach, Julia; Altmannsberger, Hans-Michael; Illies, Christopher; Luo, Xiaohua; Harvey-Peredo, Inti; Jäger, Elke; Dodoo, Ernest; Maeurer, Markus

    2018-02-01

    The prognosis for patients with glioblastoma is grim. Ex vivo expanded tumor-associated antigen (TAA)-reactive T-cells from patients with glioma may represent a viable source for anticancer-directed cellular therapies. Immunohistochemistry was used to test the survivin (n = 40 samples) and NY-ESO-1 (n = 38 samples) protein expression in tumor specimens. T-cells from peripheral blood were stimulated with TAAs (synthetic peptides) in IL-2 and IL-7, or using a combination of IL-2, IL-15 and IL-21. CD4 + and CD8 + T-cells were tested for antigen-specific proliferation by flow cytometry, and IFN-γ production was tested by ELISA. Twenty-eight out of 38 cancer specimens exhibited NY-ESO-1 protein expression, 2/38 showed a strong universal (4+) NY-ESO-1 staining, and 9/40 cancer lesions exhibited a strong (4+) staining for survivin. We could detect antigen-specific IFN-γ responses in 25% blood samples for NY-ESO-1 and 30% for survivin. NY-ESO-1-expanded T-cells recognized naturally processed and presented epitopes. NY-ESO-1 or survivin expression in glioma represents viable targets for anticancer-directed T-cells for the biological therapy of patients with glioma.

  2. Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8(+) T cells.

    Science.gov (United States)

    Pollack, Seth M; Jones, Robin L; Farrar, Erik A; Lai, Ivy P; Lee, Sylvia M; Cao, Jianhong; Pillarisetty, Venu G; Hoch, Benjamin L; Gullett, Ashley; Bleakley, Marie; Conrad, Ernest U; Eary, Janet F; Shibuya, Kendall C; Warren, Edus H; Carstens, Jason N; Heimfeld, Shelly; Riddell, Stanley R; Yee, Cassian

    2014-01-01

    Adoptive T cell therapy represents an attractive modality for the treatment of patients with cancer. Peripheral blood mononuclear cells have been used as a source of antigen specific T cells but the very low frequency of T cells recognizing commonly expressed antigens such as NY-ESO-1 limit the applicability of this approach to other solid tumors. To overcome this, we tested a strategy combining IL-21 modulation during in vitro stimulation with first-in-class use of tetramer-guided cell sorting to generate NY-ESO-1 specific cytotoxic T lymphocytes (CTL). CTL generation was evaluated in 6 patients with NY-ESO-1 positive sarcomas, under clinical manufacturing conditions and characterized for phenotypic and functional properties. Following in vitro stimulation, T cells stained with NY-ESO-1 tetramer were enriched from frequencies as low as 0.4% to >90% after single pass through a clinical grade sorter. NY-ESO-1 specific T cells were generated from all 6 patients. The final products expanded on average 1200-fold to a total of 36 billion cells, were oligoclonal and contained 67-97% CD8(+), tetramer(+) T cells with a memory phenotype that recognized endogenous NY-ESO-1. This study represents the first series using tetramer-guided cell sorting to generate T cells for adoptive therapy. This approach, when used to target more broadly expressed tumor antigens such as WT-1 and additional Cancer-Testis antigens will enhance the scope and feasibility of adoptive T cell therapy.

  3. A Cancer Vaccine Induces Expansion of NY-ESO-1-Specific Regulatory T Cells in Patients with Advanced Melanoma

    Science.gov (United States)

    Ebert, Lisa M.; MacRaild, Sarah E.; Zanker, Damien; Davis, Ian D.

    2012-01-01

    Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg), which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIXTM cancer vaccine. All patients tested had Treg (CD25bright FoxP3+ CD127neg) specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1157–170 epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1115–132 peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials. PMID:23110239

  4. Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC.

    Science.gov (United States)

    Klippel, Z K; Chou, J; Towlerton, A M; Voong, L N; Robbins, P; Bensinger, W I; Warren, E H

    2014-03-01

    Adoptive immunotherapy of tumors with T cells specific for the cancer-testis antigen NY-ESO-1 has shown great promise in preclinical models and in early stage clinical trials. Tumor persistence or recurrence after NY-ESO-1-specific therapy occurs, however, and the mechanisms of recurrence remain poorly defined. In a murine xenograft model of NY-ESO-1(+) multiple myeloma, we observed tumor recurrence after adoptive transfer of CD8(+) T cells genetically redirected to the prototypic NY-ESO-1157-165 peptide presented by HLA-A*02:01. Analysis of the myeloma cells that had escaped from T-cell control revealed intact expression of NY-ESO-1 and B2M, but selective, complete loss of HLA-A*02:01 expression from the cell surface. Loss of heterozygosity (LOH) in the major histocompatibility complex (MHC) involving the HLA-A locus was identified in the tumor cells, and further analysis revealed selective loss of the allele encoding HLA-A*02:01. Although LOH involving the MHC has not been described in myeloma patients with persistent or recurrent disease after immune therapies such as allogeneic hematopoietic cell transplantation (HCT), it has been described in patients with acute myelogenous leukemia who relapsed after allogeneic HCT. These results suggest that MHC loss should be evaluated in patients with myeloma and other cancers who relapse after adoptive NY-ESO-1-specific T-cell therapy.

  5. Somerset County Renewable Energy Initiative

    Energy Technology Data Exchange (ETDEWEB)

    Katula, Denise [County of Somerset, Somervile, NJ (United States)

    2014-05-07

    The County of Somerset, New Jersey, through the Somerset County Improvement Authority (SCIA), applied Federal funding through the U.S. Department of Energy to will apply project funds to buy-down the capital costs of equipment associated with the installation of solar photovoltaic (PV) systems at two sites owned by the County. This Renewable Energy Initiative allows the County to take advantage of clean renewable energy, without any adverse debt impacts, and at a price that results in operating budget savings beyond what is presently available in the marketplace. This project addressed the objectives of the Office of Energy Efficiency and Renewable Energy by making the acquisition of renewable energy more affordable for the County, thereby, encouraging other counties and local units to develop similar programs and increase the deployment of solar energy technologies. The two sites that were funded by the DOE grant are part of a much larger, ambitious, and unique renewable energy project, described in the next section.

  6. Hydrology of Lake County, Florida

    Science.gov (United States)

    Knochenmus, Darwin D.; Hughes, G.H.

    1976-01-01

    Lake County includes a 1,150 square-mile area consisting of ridges, uplands, and valleys in central-peninsular Florida. About 32 percent of the county is covered by lakes, swamps, and marshes. Water requirements in 1970 averaged about 54 million gallons per day. About 85 percent of the water was obtained from wells; about 15 percent from lakes. The Floridan aquifer supplies almost all the ground water used in Lake County. Annual recharge to the Floridan aquifer averages about 7 inches over the county; runoff average 8.5 inches. The quality of ground and surface water in Lake County is in general good enough for most uses; however, the poor quality of Floridan-aquifer water in the St. John River Valley probably results from the upward movement of saline water along a fault zone. Surface water in Lake County is usually less mineralized than ground water but is more turbid and colored. (Woodard-USGS)

  7. Efficient in vivo priming by vaccination with recombinant NY-ESO-1 protein and CpG in antigen naive prostate cancer patients.

    Science.gov (United States)

    Karbach, Julia; Neumann, Antje; Atmaca, Akin; Wahle, Claudia; Brand, Kathrin; von Boehmer, Lotta; Knuth, Alexander; Bender, Armin; Ritter, Gerd; Old, Lloyd J; Jäger, Elke

    2011-02-15

    NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 protein combined with CpG as adjuvant were evaluated. In a phase I clinical study, patients with advanced prostate cancer were vaccinated with recombinant NY-ESO-1 protein (100 μg) mixed with CpG 7909 (2.5 mg) every 3 weeks intradermally for 4 doses. Objectives of the study were the safety of the vaccine and changes of specific humoral and cellular immunological responses to NY-ESO-1 in relation to detectable NY-ESO-1 expression in the individual tumor. All 12 baseline sero-negative patients developed high-titer NY-ESO-1 antibody responses. B-cell epitope mapping identified NY-ESO-1 p91-110 to be recognized most frequently by vaccine-induced antibodies. Two patients developed significant antibody titers against the adjuvant CpG. NY-ESO-1-specific CD4+ and/or CD8+ T-cell responses were induced in 9 patients (69%). Five of these 9 patients did not express NY-ESO-1 in the autologous tumor. Postvaccine CD8+ T-cell clones recognized and lyzed HLA-matched tumor cell lines in an antigen-specific manner. Our data provide clear evidence for the capacity of NY-ESO-1 protein/CpG vaccine to induce integrated antigen-specific immune responses in vivo and to efficiently prime CD8+ T-cell responses in NY-ESO-1 antigen-negative patients. Our results may also support further clinical vaccination protocols with NY-ESO-1 protein not only focused on the treatment of existing cancer, but also to prevent further development of NY-ESO-1 positive cancers in vivo. ©2010 AACR.

  8. [Immune effects of specific CTLs response induced by dendritic cells pulsed with NY-ESO-1 peptide].

    Science.gov (United States)

    Liu, J W; Lu, X; Yang, Z M; Deng, L J; Yang, L

    2017-10-18

    To explore the potential of autologous dendritic cells (DCs) pulsed with caner/testis antigen NY-ESO-1 peptides in inducing specific cytotoxic T lymphocyte (CTLs) response and antineoplastic immune function of specific CTLs. Fifteen patients with II to III stage positive HLA -A0201 + and NY-ESO-1 + were enrolled in the Cancer Hospital Chinese Academy of Medical Sciences on the basis of preclinical experiments from November 2014 to October 2015, and their peripheral blood mononuclear cells (PBMCs) and peripheral blood lymphocytes (PBLs) were isolated. The PBMCs were induced into DCs and pulsed with NY-ESO-1 peptide. The phenotypes of DCs were stained with antibodies against HLA-DR + CD11c + ,CD80 + ,CD83 + and CD86 + , and subsequently analyzed by multichannel flow cytometry (FCM). The killing effects of CTLs pulsed with HLA-A0201-binding peptide NY-ESO-1 and the potential of autologous DCs pulsed with NY-ESO-1 peptides in inducing specific cytotoxic T lymphocytes (CTLs) responses were determined. The patients were administered two infusions of auto-logous CTLs for 1 time every two weeks. The total infusion was with 2 times. The immunological responses and clinical responses were examined in 1 week after the final administration. The immunophenotype of DCs pulsed with NY-ESO-1 peptide was analyzed, HLA-DR + CD11c + cells (93.6%±1.2%), CD80 + cells (87.3%±3.6%), CD83 + cells (82.8%±2.5%) and CD86 + cells (93.4%±6.4%). PBLs isolated from patients primed by DCs pulsed with NY-ESO-1 peptide proliferated continuously and the proliferation index (PI) of the PBLs were analyzed. There was significant difference between the DCs loaded with polypeptides and those unloaded, though it could promote the proliferation of PBLs, but the PI was significantly lower than that of the DCs loaded with NY-ESO-1 peptide (PESO-1 peptides was significantly higher than that in the control group (5.2%±1.2% vs. 0.4%±0.1%). CTLs induced by NY-ESO-1 pulsed DCs exerted a stronger killing

  9. Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide.

    Science.gov (United States)

    Eikawa, Shingo; Kakimi, Kazuhiro; Isobe, Midori; Kuzushima, Kiyotaka; Luescher, Immanuel; Ohue, Yoshihiro; Ikeuchi, Kazuhiro; Uenaka, Akiko; Nishikawa, Hiroyoshi; Udono, Heiichiro; Oka, Mikio; Nakayama, Eiichi

    2013-01-15

    Immunogenicity of a long 20-mer NY-ESO-1f peptide vaccine was evaluated in a lung cancer patient TK-f01, immunized with the peptide with Picibanil OK-432 and Montanide ISA-51. We showed that internalization of the peptide was necessary to present CD8 T-cell epitopes on APC, contrasting with the direct presentation of the short epitope. CD8 T-cell responses restricted to all five HLA class I alleles were induced in the patient after the peptide vaccination. Clonal analysis showed that B*35:01 and B*52:01-restricted CD8 T-cell responses were the two dominant responses. The minimal epitopes recognized by A*24:02, B*35:01, B*52:01 and C*12:02-restricted CD8 T-cell clones were defined and peptide/HLA tetramers were produced. NY-ESO-1 91-101 on A*24:02, NY-ESO-1 92-102 on B*35:01, NY-ESO-1 96-104 on B*52:01 and NY-ESO-1 96-104 on C*12:02 were new epitopes first defined in this study. Identification of the A*24:02 epitope is highly relevant for studying the Japanese population because of its high expression frequency (60%). High affinity CD8 T-cells recognizing tumor cells naturally expressing the epitopes and matched HLA were induced at a significant level. The findings suggest the usefulness of a long 20-mer NY-ESO-1f peptide harboring multiple CD8 T-cell epitopes as an NY-ESO-1 vaccine. Characterization of CD8 T-cell responses in immunomonitoring using peptide/HLA tetramers revealed that multiple CD8 T-cell responses comprised the dominant response. Copyright © 2012 UICC.

  10. Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients.

    Science.gov (United States)

    Kageyama, Shinichi; Wada, Hisashi; Muro, Kei; Niwa, Yasumasa; Ueda, Shugo; Miyata, Hiroshi; Takiguchi, Shuji; Sugino, Sahoko H; Miyahara, Yoshihiro; Ikeda, Hiroaki; Imai, Naoko; Sato, Eiichi; Yamada, Tomomi; Osako, Masaharu; Ohnishi, Mami; Harada, Naozumi; Hishida, Tadashi; Doki, Yuichiro; Shiku, Hiroshi

    2013-10-05

    Cholesteryl pullulan (CHP) is a novel antigen delivery system for cancer vaccines. This study evaluated the safety, immune responses and clinical outcomes of patients who received the CHP-NY-ESO-1 complex vaccine, Drug code: IMF-001. Patients with advanced/metastatic esophageal cancer were enrolled and subcutaneously vaccinated with either 100 μg or 200 μg of NY-ESO-1 protein complexed with CHP. The primary endpoints were safety and humoral immune responses, and the secondary endpoint was clinical efficacy. A total of 25 patients were enrolled. Thirteen and twelve patients were repeatedly vaccinated with 100 μg or 200 μg of CHP-NY-ESO-1 with a median of 8 or 9.5 doses, respectively. No serious adverse events related to the vaccine were observed. Three out of 13 patients in the 100-μg cohort and 7 out of 12 patients in the 200-μg cohort were positive for anti-NY-ESO-1 antibodies at baseline. In the 100-μg cohort, an antibody response was observed in 5 out of 10 pre-antibody-negatives patients, and the antibody levels were augmented in 2 pre-antibody-positive patients after vaccination. In the 200-μg cohort, all 5 pre-antibody-negative patients became seropositive, and the antibody level was amplified in all 7 pre-antibody-positive patients. No tumor shrinkage was observed. The patients who received 200 μg of CHP-NY-ESO-1 survived longer than patients receiving 100 μg of CHP-NY-ESO-1, even those who exhibited unresponsiveness to previous therapies or had higher tumor burdens. The safety and immunogenicity of CHP-NY-ESO-1 vaccine were confirmed. The 200 μg dose more efficiently induced immune responses and suggested better survival benefits. (Clinical trial registration number NCT01003808).

  11. Case study: Khoramdareh County

    Directory of Open Access Journals (Sweden)

    Vahid Riahi Riahi

    2016-10-01

    Full Text Available Environmental sustainability of rural settlements based on a systematic viewpoint may be defined as a realization of sustainable development in different social, economic and environmental aspects of rural areas. Achieving this goal requires that we pay more attention to effective elements and factors through a set of sustainability indices. This research was meant to analyze sustainable factors of rural settlement in three dimensions: environmental, social and economic context using multi-criteria decision analysis and explanation of the relationships between its active and effective factors in the rural area of the Khorramdarreh County in the province of Zanjan. The research method used is the descriptive analytic approach. Data from 287 households were sampled randomly from a total of 1143 households in the four villages including: Rahmat Abad, Alvand, Baghdareh and, Sukhariz (out of 15 villages in the Khorramdarreh County. In the process of doing this research and after calculating the weights, the difference in the sustainability of environmental, social, economic and physical aspects in rural areas of this county have been determined. Data was collected using library and field research through questionnaires. Data analysis was performed by the One-Sample t Test and the Vikur and path analysis techniques, using statistical software SPSS. The findings show that environmental sustainability in the study area is half desirable. Among the different aspects of environmental sustainability, the most effective factors are physical, economic, social and environmental aspects, respectively. Little attention of policy-making –system to socio-cultural and environmental aspects, especially in practice, and rapid and unplanned utilization of production resources are the most important factors affecting this situation in two given dimensions. Although, in programmed documents the planning system agents emphasize on the socio-cultural sustainability

  12. Cancer-testis antigen expression in synovial sarcoma: NY-ESO-1, PRAME, MAGEA4, and MAGEA1.

    Science.gov (United States)

    Iura, Kunio; Maekawa, Akira; Kohashi, Kenichi; Ishii, Takeaki; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Harimaya, Katsumi; Iwamoto, Yukihide; Oda, Yoshinao

    2017-03-01

    Synovial sarcoma (SS) is regarded as a relatively chemosensitive sarcoma, but the prognosis of advanced SSs remains poor. Here we identified highly expressed cancer-testis antigens that could be promising immunotherapy targets for SS, using a previously conducted cDNA microarray, and we assessed the clinicopathological or prognostic relationships of these antigens in SS. We compared the gene expression profiles of 11 SSs with those of 3 normal adipose tissues. Among the up-regulated cancer-testis antigens, we analyzed PRAME, MAGEA1, and MAGEA4 and another cancer-testis antigen (NY-ESO-1) together, by immunohistochemistry and real-time polymerase chain reaction in 108 SSs. Immunohistochemically, NY-ESO-1, PRAME, MAGEA4, and MAGEA1 were positive in 66 (61%), 93 (86%), 89 (82%), and 16 (15%) of 108 SSs, respectively, and 104 (96%) of 108 SSs showed the immunohistochemical expression of at least 1 of NY-ESO-1, PRAME, and MAGEA4. Moreover, the high expression of at least 1 of these 3 antigens was observed in 83% of the SSs. High expression of NY-ESO-1 and MAGEA4 was significantly correlated with the presence of necrosis and advanced clinical stage. The immunohistochemical expression of these cancer-testis antigens was not correlated with prognosis, but the coexpression of NY-ESO-1, PRAME, and MAGEA4 was significantly associated with adverse prognosis. The real-time polymerase chain reaction results were closely related to the immunohistochemical results: NY-ESO-1 (P = .0019), PRAME (P = .039), MAGEA4 (P = .0149), and MAGEA1 (P = .0766). These data support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as immunotherapy targets and ancillary prognostic parameters, suggesting the possible benefit of the combined use of these cancer-testis antigens as an SS immunotherapy target. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Combination of autoantibodies against NY-ESO-1 and viral capsid antigen immunoglobulin A for improved detection of nasopharyngeal carcinoma.

    Science.gov (United States)

    Peng, Yu-Hui; Xu, Yi-Wei; Qiu, Si-Qi; Hong, Chao-Qun; Zhai, Tian-Tian; Li, En-Min; Xu, Li-Yan

    2014-09-01

    Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southern China and Southeast Asia, and early detection remains a challenge. Autoantibodies have been found to precede the manifestations of symptomatic cancer by several months to years, making their identification of particular relevance for early detection. In the present study, the diagnostic value of serum autoantibodies against NY-ESO-1 in NPC patients was evaluated. The study included 112 patients with NPC and 138 normal controls. Serum levels of autoantibodies against NY-ESO-1 and classical Epstein-Barr virus marker, viral capsid antigen immunoglobulin A (VCA-IgA), were measured by enzyme-linked immunosorbent assay. Measurement of autoantibodies against NY-ESO-1 and VCA-IgA demonstrated a sensitivity/specificity of 42.9/94.9% [95% confidence interval (CI), 33.7-52.6/89.4-97.8%] and 55.4/95.7% (95% CI, 45.7-64.7/90.4-98.2%), respectively. The area under receiver operating characteristic curve for autoantibodies against NY-ESO-1 (0.821; 95% CI, 0.771-0.871) was marginally lower than that for VCA-IgA (0.860; 95% CI, 0.810-0.910) in NPC. The combination of autoantibodies against NY-ESO-1 and VCA-IgA yielded an enhanced sensitivity of 80.4% (95% CI, 71.6-87.0%) and a specificity of 90.6% (95% CI, 84.1-94.7%). Moreover, detection of autoantibodies against NY-ESO-1 could differentiate early-stage NPC patients from normal controls. Our results suggest that autoantibodies against NY-ESO-1 may serve as a potential biomarker, as a supplement to VCA-IgA, for the screening and diagnosis of NPC.

  14. Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation.

    Science.gov (United States)

    Luetkens, Tim; Kobold, Sebastian; Cao, Yanran; Ristic, Marina; Schilling, Georgia; Tams, Sinje; Bartels, Britta Marlen; Templin, Julia; Bartels, Katrin; Hildebrandt, York; Yousef, Sara; Marx, Andreas; Haag, Friedrich; Bokemeyer, Carsten; Kröger, Nicolaus; Atanackovic, Djordje

    2014-11-01

    Multiple myeloma (MM) is the malignancy with the most frequent expression of the highly immunogenic cancer-testis antigens (CTA), and we have performed the first analysis of longitudinal expression, immunological properties, and fine specificity of CTA-specific antibody responses in MM. Frequency and characteristics of antibody responses against cancer-testis antigens MAGE-A3, NY-ESO-1, PRAME, and SSX-2 were analyzed using peripheral blood (N = 1094) and bone marrow (N = 200) plasma samples from 194 MM patients. We found that antibody responses against CTA were surprisingly rare, only 2.6 and 3.1 % of patients evidenced NY-ESO-1- and SSX-2-specific antibodies, respectively. NY-ESO-1-specific responses were observed during disease progression, while anti-SSX-2 antibodies appeared after allogeneic stem cell transplantation and persisted during clinical remission. We found that NY-ESO-1- and SSX-2-specific antibodies were both capable of activating complement and increasing CTA uptake by antigen-presenting cells. SSX-2-specific antibodies were restricted to IgG3, NY-ESO-1 responses to IgG1 and IgG3. Remarkably, NY-ESO-1-positive sera recognized various non-contiguous regions, while SSX-2-specific responses were directed against a single 6mer epitope, SSX-2(85-90). We conclude that primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional. While their contribution to disease control still remains unclear, our data demonstrate their theoretic ability to affect cellular anti-tumor immunity by formation and uptake of mono- and polyvalent immune complexes.

  15. Identification of NY-BR-1-specific CD4(+) T cell epitopes using HLA-transgenic mice.

    Science.gov (United States)

    Gardyan, Adriane; Osen, Wolfram; Zörnig, Inka; Podola, Lilli; Agarwal, Maria; Aulmann, Sebastian; Ruggiero, Eliana; Schmidt, Manfred; Halama, Niels; Leuchs, Barbara; von Kalle, Christof; Beckhove, Philipp; Schneeweiss, Andreas; Jäger, Dirk; Eichmüller, Stefan B

    2015-06-01

    Breast cancer represents the second most common cancer type worldwide and has remained the leading cause of cancer-related deaths among women. The differentiation antigen NY-BR-1 appears overexpressed in invasive mammary carcinomas compared to healthy breast tissue, thus representing a promising target antigen for T cell based tumor immunotherapy approaches. Since efficient immune attack of tumors depends on the activity of tumor antigen-specific CD4(+) effector T cells, NY-BR-1 was screened for the presence of HLA-restricted CD4(+) T cell epitopes that could be included in immunological treatment approaches. Upon NY-BR-1-specific DNA immunization of HLA-transgenic mice and functional ex vivo analysis, a panel of NY-BR-1-derived library peptides was determined that specifically stimulated IFNγ secretion among splenocytes of immunized mice. Following in silico analyses, four candidate epitopes were determined which were successfully used for peptide immunization to establish NY-BR-1-specific, HLA-DRB1*0301- or HLA-DRB1*0401-restricted CD4(+) T cell lines from splenocytes of peptide immunized HLA-transgenic mice. Notably, all four CD4(+) T cell lines recognized human HLA-DR-matched dendritic cells (DC) pulsed with lysates of NY-BR-1 expressing human tumor cells, demonstrating natural processing of these epitopes also within the human system. Finally, CD4(+) T cells specific for all four CD4(+) T cell epitopes were detectable among PBMC of breast cancer patients, showing that CD4(+) T cell responses against the new epitopes are not deleted nor inactivated by self-tolerance mechanisms. Our results present the first NY-BR-1-specific HLA-DRB1*0301- and HLA-DRB1*0401-restricted T cell epitopes that could be exploited for therapeutic intervention against breast cancer. © 2014 UICC.

  16. 2015 Resident Survey (City and County)

    Data.gov (United States)

    City and County of Durham, North Carolina — The purpose of the annual City/County survey: To objectively assess citizen satisfaction with the delivery of City/County servicesTo set a baseline for future...

  17. 2016 Resident Survey (City and County)

    Data.gov (United States)

    City and County of Durham, North Carolina — The purpose of the annual City/County survey: To objectively assess citizen satisfaction with the delivery of City/County servicesTo set a baseline for future...

  18. Allegheny County Beltway System Street Centerlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Authoritative dataset of the beltway system in Allegheny County. The system was developed to help motorists navigate through Allegheny County on low-traffic roads....

  19. 2006 Fulton County Georgia Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Light Detection and Ranging (LiDAR) LAS dataset is a survey of Fulton County. The Fulton County LiDAR Survey project area consists of approximately 690.5 square...

  20. Two novel poxviruses with unusual genome rearrangements: NY_014 and Murmansk.

    Science.gov (United States)

    Smithson, Chad; Meyer, Hermann; Gigante, Crystal M; Gao, Jinxin; Zhao, Hui; Batra, Dhwani; Damon, Inger; Upton, Chris; Li, Yu

    2017-12-01

    The genome sequence and annotation of two novel poxviruses, NY_014 and Murmansk, are presented. Despite being isolated on different continents and from different hosts, the viruses are relatively similar, albeit distinct species. The closest known relative of the novel viruses is Yoka poxvirus. Five novel genes were found in these genomes, two of which were MHC class I homologs. Although the core of these genomes was well conserved, the terminal regions showed significant variability with large deletions and surprising evidence of recombination with orthopoxviruses.

  1. Primes of the form x2+ny2 Fermat, class field theory, and complex multiplication

    CERN Document Server

    Cox, David A

    2014-01-01

    An exciting approach to the history and mathematics of number theory ". . . the author's style is totally lucid and very easy to read . . .the result is indeed a wonderful story." -Mathematical ReviewsWritten in a unique and accessible style for readers of varied mathematical backgrounds, the Second Edition of Primes of the Form p = x2+ ny2 details the history behind how Pierre de Fermat's work ultimately gave birth to quadratic reciprocity and the genus theory of quadratic forms. The book also illustrates how results of Euler and Gauss can be fully understood only in the context of class fi

  2. Human impact on tundra environment at the Ny-Ålesund Station, Svalbard

    OpenAIRE

    Jadwiga Krzyszowska, Anna

    1989-01-01

    Studies were performed in Ny-Alesund, Spitsbergen, in June and July 1986 in order to gain an insight into the effect of activities of the polar research station on the nearby environment. It was found that chemical and mechanical factors were the most detrimental to the tundra environment. Fuel oil spills (110 m3 in 1986), which spread via surface and ground waters, were the most damaging of the chemical factors. Domestic sewage polluted the waters of Kolhamna Bay within an area of only 0.5 h...

  3. Rugby-metoden SCRUM: En ny måde at bedrive projekter på

    DEFF Research Database (Denmark)

    Pries-Heje, Lene; Pries-Heje, Jan

    2012-01-01

    En ny måde at bedrive projekter på breder sig i disse år med overraskende fart. Selv om de første tanker om ”Scrum” – hvor navnet og fremgangsmåden er inspireret af Rugby-spillet – blev tænkt for mere end 10 år siden, så er det først nu at fremgangsmåden for alvor har vundet udbredelse i Danmark...

  4. Vurdering av strømforhold og partikkelspredning ved etablering av ny dypvannskai ved Rana Industriterminal

    OpenAIRE

    Staalstrøm, Andre; Kempa, Magdalena

    2015-01-01

    Det skal etableres en ny dypvannskai ved Rana Industriterminal, og i den forbindelse har NIVA gjort vurderinger av strømforhold i området og partikkelspredning i anleggsperioden. I denne rapporten er det tatt utgangspunkt i at 135 000 m³ med masse skal mudres opp. Det er videre antatt at 2 % av denne massen spres i Ranfjorden, og dette utgjør 1620 tonn tørr masse. Denne mengden tilsvarer den samme mengden partikler som kan tilføres fjorden fra Ranelva i løpet av en ukes tid, og anses ikke som...

  5. Seismic analysis of the nuclear fuel service reprocessing plant at West Valley, N.Y

    International Nuclear Information System (INIS)

    Murray, R.C.; Nelson, T.A.; Davito, A.M.

    1977-01-01

    This report was prepared at the request of the Nuclear Regulatory Commission as a part of a license review of the nuclear fuel reprocessing plant operated by Nuclear Fuel Services, Inc., at West Valley, N.Y. The report discusses the approach used by the Lawrence Livermore Laboratory in performing an independent seismic analysis of the facility. It includes a description of the facility, our modeling and analysis techniques, failure criteria, results, and conclusions. Two modes of failure are identified and their consequences discussed

  6. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Directory of Open Access Journals (Sweden)

    Konkankit Veerauo V

    2011-11-01

    Full Text Available Abstract Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis.

  7. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Science.gov (United States)

    2011-01-01

    Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA) in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis. PMID:22060015

  8. Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells.

    Science.gov (United States)

    Mobergslien, Anne; Vasovic, Vlada; Mathiesen, Geir; Fredriksen, Lasse; Westby, Phuong; Eijsink, Vincent G H; Peng, Qian; Sioud, Mouldy

    2015-01-01

    Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

  9. Thermal stability of atomic layer deposited WCxNy electrodes for metal oxide semiconductor devices

    Science.gov (United States)

    Zonensain, Oren; Fadida, Sivan; Fisher, Ilanit; Gao, Juwen; Danek, Michal; Eizenberg, Moshe

    2018-01-01

    This study is a thorough investigation of the chemical, structural, and electrical stability of W based organo-metallic films, grown by atomic layer deposition, for future use as gate electrodes in advanced metal oxide semiconductor structures. In an earlier work, we have shown that high effective work-function (4.7 eV) was produced by nitrogen enriched films (WCxNy) dominated by W-N chemical bonding, and low effective work-function (4.2 eV) was produced by hydrogen plasma resulting in WCx films dominated by W-C chemical bonding. In the current work, we observe, using x-ray diffraction analysis, phase transformation of the tungsten carbide and tungsten nitride phases after 900 °C annealing to the cubic tungsten phase. Nitrogen diffusion is also observed and is analyzed with time-of-flight secondary ion mass spectroscopy. After this 900 °C anneal, WCxNy effective work function tunability is lost and effective work-function values of 4.7-4.8 eV are measured, similar to stable effective work function values measured for PVD TiN up to 900 °C anneal. All the observed changes after annealing are discussed and correlated to the observed change in the effective work function.

  10. Orbital Period Variations in the NY Vir System, Revisited in the Light of New Data

    Directory of Open Access Journals (Sweden)

    Baştürk Özgür

    2018-02-01

    Full Text Available NY Virginis is an eclipsing binary system with a subdwarf B primary and an M type dwarf secondary. Recent studies (Qian et al. 2012; Lee et al. 2014 suggested the presence of two circumbinary planets with a few Jovian masses within the system. Lee et al. (2014 examined the orbital stabilities of the suggested planets, using the best-fit parameters derived from their eclipse timing variation analysis. They found that the outer companion should be ejected from the system in about 800 000 years. An observational report from Pulley et al. (2016 pointed out that the recent mideclipse times of the binary deviate significantly from the models suggested by Lee et al. (2014. In fact, variations in the orbital period of the system had already been recognized by many authors, but the parameters of these variations vary significantly as new data accumulate. Here, we analyze the eclipse timing variations of the NY Vir system, using new mid-eclipse times that we have obtained together with earlier published measurements in order to understand the nature of the system and constrain its parameters.

  11. MAGE-A1, GAGE and NY-ESO-1 cancer/testis antigen expression during human gonadal development

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Kock, Kirsten; Nielsen, Ole

    2007-01-01

    BACKGROUND: Cancer/testis antigens (CTAs) are expressed in several cancers and during normal adult male germ cell differentiation. Little is known about their role in fetal development of human germ cells. METHODS: We examined expression of the CTAs MAGE-A1, GAGE and NY-ESO-1 in fetal gonads...... with highly proliferating germ cells, whereas GAGE proteins have a more general function in germ cells unrelated to any specific developmental stage. The recognition of differential cellular expression of GAGE, MAGE-A1, NY-ESO-1 and OCT4 may help define biologically distinct germ cell subpopulations....... cells and the staining intensity of all three CTAs peaked during the second trimester and gradually decreased towards birth in both male and female germ cells. In oocytes, MAGE-A1 expression terminated around birth, whereas NY-ESO-1 expression persisted through the neonatal stage and GAGE expression...

  12. A mutation in the envelope protein fusion loop attenuates mouse neuroinvasiveness of the NY99 strain of West Nile virus

    International Nuclear Information System (INIS)

    Zhang Shuliu; Li Li; Woodson, Sara E.; Huang, Claire Y.-H.; Kinney, Richard M.; Barrett, Alan D.T.; Beasley, David W.C.

    2006-01-01

    Substitutions were engineered individually and in combinations at the fusion loop, receptor-binding domain and a stem-helix structure of the envelope protein of a West Nile virus strain, NY99, and their effects on mouse virulence and presentation of epitopes recognized by monoclonal antibodies (MAbs) were assessed. A single substitution within the fusion loop (L107F) attenuated mouse neuroinvasiveness of NY99. No substitutions attenuated NY99 neurovirulence. The L107F mutation also abolished binding of a non-neutralizing MAb, 3D9, whose epitope had not been previously identified. MAb 3D9 was subsequently shown to be broadly cross-reactive with other flaviviruses, consistent with binding near the highly conserved fusion loop

  13. Measurement of serum antibodies against NY-ESO-1 by ELISA: A guide for the treatment of specific immunotherapy for patients with advanced colorectal cancer.

    Science.gov (United States)

    Long, Yan-Yan; Wang, Yu; Huang, Qian-Rong; Zheng, Guang-Shun; Jiao, Shun-Chang

    2014-10-01

    NY-ESO-1 has been identified as one of the most immunogenic antigens; thus, is a highly attractive target for cancer immunotherapy. The present study analyzed the expression of serum antibodies (Abs) against NY-ESO-1 in patients with advanced colorectal cancer (CRC), with the aim of guiding the treatment of NY-ESO-1-based specific-immunotherapy for these patients. Furthermore, the present study was the first to evaluate the kinetic expression of anti-NY-ESO-1 Abs and investigate the possible influencing factors. A total of 239 serum samples from 155 pathologically confirmed patients with advanced CRC (stages III and IV) were collected. The presence of spontaneous Abs against NY-ESO-1 was analyzed using an enzyme-linked immunosorbent assay (ELISA). The results demonstrated that 24.5% (38/155) of the investigated patients were positive for NY-ESO-1-specific Abs. No statistically significant correlations were identified between the expression of anti-NY-ESO-1 Abs and clinicopathological parameters, including age and gender, location, grading, local infiltration, lymph node status, metastatic status and K-ras mutation status (P>0.05). In 59 patients, the kinetic expression of anti-NY-ESO-1 Abs was analyzed, of which 14 patients were initially positive and 45 patients were initially negative. Notably, 16/59 (27.1%) patients changed their expression status during the study period, and the initially positive patients were more likely to change compared with the initially negative patients (85.7 vs. 8.8%; PESO-1 by ELISA is an easy and feasible method. The high expression rate of NY-ESO-1-specific Abs in CRC patients indicates that measuring the levels of serum Abs against NY-ESO-1 may guide the treatment of NY-ESO-1-based specific immunotherapy for patients with advanced CRC.

  14. Integrated NY-ESO-1 antibody and CD8+ T-cell responses correlate with clinical benefit in advanced melanoma patients treated with ipilimumab.

    Science.gov (United States)

    Yuan, Jianda; Adamow, Matthew; Ginsberg, Brian A; Rasalan, Teresa S; Ritter, Erika; Gallardo, Humilidad F; Xu, Yinyan; Pogoriler, Evelina; Terzulli, Stephanie L; Kuk, Deborah; Panageas, Katherine S; Ritter, Gerd; Sznol, Mario; Halaban, Ruth; Jungbluth, Achim A; Allison, James P; Old, Lloyd J; Wolchok, Jedd D; Gnjatic, Sacha

    2011-10-04

    Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity to NY-ESO-1, a cancer/testis antigen expressed in a subset of patients with melanoma. To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum antibody by ELISA in 144 ipilimumab-treated patients with melanoma and found 22 of 140 (16%) seropositive at baseline and 31 of 144 (22%) seropositive following treatment. These NY-ESO-1-seropositive patients had a greater likelihood of experiencing clinical benefit 24 wk after ipilimumab treatment than NY-ESO-1-seronegative patients (P = 0.02, relative risk = 1.8, two-tailed Fisher test). To understand why some patients with NY-ESO-1 antibody failed to experience clinical benefit, we analyzed NY-ESO-1-specific CD4(+) and CD8(+) T-cell responses by intracellular multicytokine staining in 20 NY-ESO-1-seropositive patients and found a surprising dissociation between NY-ESO-1 antibody and CD8 responses in some patients. NY-ESO-1-seropositive patients with associated CD8(+) T cells experienced more frequent clinical benefit (10 of 13; 77%) than those with undetectable CD8(+) T-cell response (one of seven; 14%; P = 0.02; relative risk = 5.4, two-tailed Fisher test), as well as a significant survival advantage (P = 0.01; hazard ratio = 0.2, time-dependent Cox model). Together, our data suggest that integrated NY-ESO-1 immune responses may have predictive value for ipilimumab treatment and argue for prospective studies in patients with established NY-ESO-1 immunity. The current findings provide a strong rationale for the clinical use of modulators of immunosuppression with concurrent approaches to favor tumor antigen-specific immune responses, such as vaccines or adoptive transfer, in patients with cancer.

  15. Evaluation of LAGE-1 and NY-ESO-1 expression in multiple myeloma patients to explore possible benefits of their homology for immunotherapy.

    Science.gov (United States)

    de Carvalho, Fabricio; Vettore, Andre L; Inaoka, Riguel J; Karia, Bruno; Andrade, Valéria C C; Gnjatic, Sacha; Jungbluth, Achim A; Colleoni, Gisele W B

    2011-01-20

    Due to the high homology between the LAGE-1 and NY-ESO-1 proteins, we hypothesized that an anti-NY-ESO-1 vaccine might elicit LAGE-1 immunity and hence may be effective in multiple myeloma (MM) patients with LAGE-1-positive/NY-ESO-1-negative tumors. Therefore, we set out to evaluate LAGE-1 and NY-ESO-1 mRNA and protein expression in MM patients in a bid to evaluate possible benefits of their homology for immunotherapy. LAGE-1 (a and b isoforms) and NY-ESO-1 mRNA expression was studied in 18 normal tissues and 50 bone marrow MM samples by RT-PCR. LAGE-1 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in 27 MM specimens using mAbs 219-510-23 and E978. Spontaneous serological immune response against both antigens was analyzed by ELISA in sera from 33 MM patients. LAGE-1 (a and b isoforms) was positive in 42% and NY-ESO-1 in 26% of the MM samples analyzed by RT-PCR. Both genes were found to be expressed in 18% of the cases, while at least one of the genes was found to be expressed in 50% of the cases. In LAGE-1 positive samples, 81% were positive for LAGE-1a and 19% were positive for both LAGE-1a and -1b. LAGE-1 and NY-ESO-1 protein expression could only be detected in two cases by IHC and there was a clear strong spontaneous antibody response to LAGE-1 and NY-ESO-1 in only one MM patient. In conclusion, LAGE-1a and NY-ESO-1 homology cannot be easily exploited in an anti-NY-ESO-1 vaccine given the low frequency of protein expression detected by IHC or serum analysis.

  16. Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma.

    Science.gov (United States)

    Sabado, Rachel Lubong; Pavlick, Anna; Gnjatic, Sacha; Cruz, Crystal M; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A; Bhardwaj, Nina

    2015-03-01

    The Toll-like receptor (TLR) 7/8 agonist resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide (Seppic) with or without resiquimod in patients with high-risk melanoma. In part I of the study, patients received 100 μg of full-length NY-ESO-1 protein emulsified in 1.25 mL of Montanide (day 1) followed by topical application of 1,000 mg of 0.2% resiquimod gel on days 1 and 3 (cohort 1) versus days 1, 3, and 5 (cohort 2) of a 21-day cycle. In part II, patients were randomized to receive 100-μg NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel [(arm A; n = 8) or 1,000 mg of 0.2% resiquimod gel (arm B; n = 12)] using the dosing regimen established in part I. The vaccine regimens were generally well tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4⁺ T-cell responses. CD8⁺ T-cell responses were only seen in 3 of 12 patients in arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8⁺ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical resiquimod is safe and induces both antibody and CD4⁺ T-cell responses in the majority of patients; the small proportion of CD8⁺ T-cell responses suggests that the addition of topical resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8⁺ T-cell responses. ©2015 American Association for Cancer Research.

  17. Allegheny County Map Index Grid

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Map Index Sheets from Block and Lot Grid of Property Assessment and based on aerial photography, showing 1983 datum with solid line and NAD 27 with 5 second grid...

  18. Montgomery County Council Legislation - Bills

    Data.gov (United States)

    Montgomery County of Maryland — The Council enacts local public laws for the ‘peace, good government, health, and welfare of the county’. The bills dataset contains all legislation considered by...

  19. ORTHOIMAGERY, ERIE COUNTY, OHIO USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The 2006 OSIP digital orthophotography was collected during the months of March and April (leaf-off conditions). The MrSID Images covering each county at 1-foot...

  20. ORTHOIMAGERY, LICKING COUNTY, OHIO USA

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The 2006 OSIP digital orthophotography was collected during the months of March and April (leaf-off conditions). The MrSID Images covering each county at 1-foot...

  1. Uninsured Young Adults by County

    Data.gov (United States)

    U.S. Department of Health & Human Services — This data file indicates the estimated number of uninsured individuals ages 19-25 in each U.S. county. These individuals may be eligible to join their parents health...

  2. 2009 SCDNR Horry County Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sanborn Map Company completed the original classification of the multiple return LiDAR of Horry County, South Carolina in 2009. In 2013, Dewberry was tasked with...

  3. 2009 SCDNR Berkeley County Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sanborn Map Company completed the original classification of the multiple return LiDAR of Berkeley County, South Carolina in 2009. In 2013, Dewberry was tasked with...

  4. 2009 SCDNR Charleston County Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Photoscience completed the original collection and classification of the multiple return LiDAR of Charleston County, South Carolina in the winter of 2006-2007. In...

  5. Sierra County E-911 Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This data set contains a vector digital representation of all accessible roads including interstate highways, State highways, county roads and some city streets in...

  6. County Boundaries with Shorelines (National)

    Data.gov (United States)

    Department of Transportation — County boundaries with shorelines cut in (NTAD). The TIGER/Line Files are shapefiles and related database files (.dbf) that are an extract of selected geographic and...

  7. Allegheny County Illegal Dump Sites

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Illegal Dump Site dataset includes information on illegal dump sites, their type of trash, and the estimate tons of trash at each site. The information was...

  8. Soils - Volusia County Soils (Polygons)

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — Soils: 1:24000 SSURGO Map. Polygon boundaries of Soils in Volusia County, downloaded from SJRWMD and created by NRCS and SJRWMD. This data set is a digital version...

  9. 2009 SCDRN Lidar: Florence County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The South Carolina Department of Natural Resources (SCDNR) contracted with Sanborn to provide LiDAR mapping services for Florence County, SC. Utilizing multi-return...

  10. 2014 Mobile County, AL Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Atlantic was contracted to acquire high resolution topographic LiDAR (Light Detection and Ranging) data located in Mobile County, Alabama. The intent was to collect...

  11. Allegheny County Soil Type Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains soil type and soil classification, by area. Additional info at: http://mcdc.cas.psu.edu/datawiz.htm;...

  12. County business patterns, 1996 : Nevada

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  13. County business patterns, 1997 : Wisconsin

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  14. County business patterns, 1997 : Arizona

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  15. County business patterns, 1997 : Wyoming

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  16. County business patterns, 1997 : Minnesota

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  17. County business patterns, 1996 : Colorado

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  18. County business patterns, 1996 : Montana

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  19. County business patterns, 1996 : Nebraska

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  20. County business patterns, 1996 : Kentucky

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  1. County business patterns, 1996 : Idaho

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  2. County business patterns, 1997 : Alabama

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  3. County business patterns, 1997 : Maryland

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  4. County business patterns, 1997 : Maine

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  5. County business patterns, 1996 : Utah

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  6. County business patterns, 1996 : Oklahoma

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  7. County business patterns, 1996 : Alaska

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  8. County business patterns, 1997 : Kansas

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  9. County business patterns, 1997 : Florida

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  10. County business patterns, 1997 : Oklahoma

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  11. County business patterns, 1997 : Michigan

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  12. County business patterns, 1997 : Tennessee

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  13. County business patterns, 1997 : Kentucky

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  14. County business patterns, 1996 : Louisiana

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  15. County business patterns, 1997 : Texas

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  16. County business patterns, 1997 : Virginia

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  17. County business patterns, 1996 : Delaware

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  18. County business patterns, 1997 : Idaho

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  19. County business patterns, 1997 : Nevada

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  20. County business patterns, 1996 : Wisconsin

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  1. County business patterns, 1996 : Arkansas

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  2. County business patterns, 1996 : Florida

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  3. County business patterns, 1997 : Mississippi

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  4. County business patterns, 1996 : Missouri

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  5. County business patterns, 1996 : Maryland

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  6. County business patterns, 1997 : Hawaii

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  7. County business patterns, 1996 : Ohio

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  8. County business patterns, 1996 : Alabama

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  9. County business patterns, 1997 : Alaska

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  10. County business patterns, 1996 : Iowa

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  11. County business patterns, 1996 : Georgia

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  12. County business patterns, 1996 : Michigan

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  13. County business patterns, 1996 : Texas

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  14. County business patterns, 1997 : Nebraska

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  15. County business patterns, 1997 : Massachusetts

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  16. County business patterns, 1997 : Indiana

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  17. County business patterns, 1996 : Pennsylvania

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  18. County business patterns, 1997 : Arkansas

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  19. County business patterns, 1997 : Colorado

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  20. County business patterns, 1996 : Arizona

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  1. County business patterns, 1997 : Oregon

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  2. County business patterns, 1997 : Georgia

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  3. County business patterns, 1997 : Missouri

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  4. County business patterns, 1997 : Illinois

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  5. County business patterns, 1997 : Utah

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  6. County business patterns, 1996 : Kansas

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  7. County business patterns, 1996 : Maine

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  8. County business patterns, 1997 : Pennsylvania

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  9. County business patterns, 1996 : Tennessee

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  10. County business patterns, 1997 : Ohio

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  11. County business patterns, 1996 : Vermont

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  12. County business patterns, 1996 : Wyoming

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  13. County business patterns, 1996 : Illinois

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  14. County business patterns, 1996 : Virginia

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  15. County business patterns, 1997 : Iowa

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  16. County business patterns, 1997 : Connecticut

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  17. County business patterns, 1996 : Hawaii

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  18. County business patterns, 1997 : Washington

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  19. County business patterns, 1997 : Vermont

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  20. County business patterns, 1996 : Oregon

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  1. County business patterns, 1996 : Minnesota

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  2. County business patterns, 1996 : California

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  3. County business patterns, 1996 : Indiana

    Science.gov (United States)

    1998-11-01

    County Business Patterns is an annual series that : provides subnational economic data by industry. The series : is useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  4. County business patterns, 1997 : Delaware

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  5. County business patterns, 1997 : Montana

    Science.gov (United States)

    1999-09-01

    County Business Patterns is an annual series that provides : subnational economic data by industry. The series is : useful for studying the economic activity of small areas; : analyzing economic changes over time; and as a benchmark : for statistical...

  6. Allegheny County Property Assessment Appeals

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Lists property assessment appeals filed and heard with the Board of Property Assessment Appeals and Review (BPAAR) and the hearing results, for tax years 2015 to...

  7. Allegheny County Toxics Release Inventory

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Toxics Release Inventory (TRI) data provides information about toxic substances released into the environment or managed through recycling, energy recovery, and...

  8. Torrance County E-911 Roads

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — The Torrance County digital road network wa created as part of the State of New Mexico Enhanced 911 Addressing Grant. The original primary function was to lay the...

  9. Allegheny County Land Cover Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Land Cover dataset demarcates 14 land cover types by area; such as Residential, Commercial, Industrial, Forest, Agriculture, etc. If viewing this description on...

  10. Allegheny County Basin Outlines Map

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This basins dataset was created to initiate regional watershed approaches with respect to sewer rehabilitation. If viewing this description on the Western...

  11. 2009 Chatham County Georgia Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LiDAR generated point cloud acquired in spring 2009 for Chatham County, Georgia for the Metropolitan Planning Commission. The data are classified as follows: Class 1...

  12. Allegheny County Building Footprint Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains photogrammetrically compiled roof outlines of buildings. All near orthogonal corners are square. Buildings that are less than 400 square feet...

  13. Allegheny County Wooded Area Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates stands of trees (coniferous and deciduous) too numerous to plot as individual trees. The area is delineated following a generalized line...

  14. Allegheny County Environmental Justice Areas

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Environmental Justice areas in this guide have been defined by the Pennsylvania Department of Environmental Protection. The Department defines an environmental...

  15. Allegheny County WIC Vendor Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains the locations of Women, Infants, and Children (WIC) program vendors. If viewing this description on the Western Pennsylvania Regional Data...

  16. Allegheny County Certified MWDBE Businesses

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — According to the Federal Department of Transportation, Disadvantaged Business Enterprises (DBE) are for-profit small business concerns where socially and...

  17. Antigen-specific in vitro expansion of functional redirected NY-ESO-1-specific human CD8+ T-cells in a cell-free system.

    Science.gov (United States)

    Jakka, Gopinadh; Schuberth, Petra C; Thiel, Markus; Held, Gerhard; Stenner, Frank; Van Den Broek, Maries; Renner, Christoph; Mischo, Axel; Petrausch, Ulf

    2013-10-01

    Tumors can be targeted by the adoptive transfer of chimeric antigen receptor (CAR) redirected T-cells. Antigen-specific expansion protocols are needed to generate large quantities of redirected T-cells. We aimed to establish a protocol to expand functional active NY-ESO-1-specific redirected human CD8(+) T-cells. The anti-idiotypic Fab antibody A4 with specificity for HLA-A 0201/NY-ESO-1157-165 was tested by competition assays using a HLA-A 0201/NY-ESO-1157-165 tetramer. HLA-A 0201/NY-ESO-1157-165 redirected T-cells were generated, expanded and tested for CAR expression, cytokine release, in vitro cytolysis and protection against xenografted HLA-A 0201/NY-ESO-1157-165-positive multiple myeloma cells. A4 demonstrated antigen-specific binding to HLA-A 0201/NY-ESO-1157-165 redirected T-cells. Expansion with A4 resulted in 98% of HLA-A 0201/NY-ESO-1157-165 redirected T-cells. A4 induced strong proliferation, resulting in a 300-fold increase of redirected T-cells. After expansion protocols, redirected T-cells secreted Interleukin-2, (IL-2), interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) and lysed target cells in vitro and were protective in vivo. A4 expanded HLA-A 0201/NY-ESO-1157-165 redirected T-cells with preservation of antigen-specific function.

  18. From single-site tantalum complexes to nanoparticles of TaxNy and TaOxNy supported on silica: elucidation of synthesis chemistry by dynamic nuclear polarization surface enhanced NMR spectroscopy and X-ray absorption spectroscopy

    KAUST Repository

    Mohandas, Janet Chakkamadathil

    2017-06-08

    Air-stable catalysts consisting of tantalum nitride nanoparticles represented as a mixture of TaxNy and TaOxNy with diameters in the range of 0.5 to 3 nm supported on highly dehydroxylated silica were synthesized from TaMe5 (Me = methyl) and dimeric Ta-2(OMe)(10) with guidance by the principles of surface organometallic chemistry (SOMC). Characterization of the supported precursors and the supported nanoparticles formed from them was carried out by IR, NMR, UV-Vis, extended X-ray absorption fine structure, and X-ray photoelectron spectroscopies complemented with XRD and high-resolution TEM, with dynamic nuclear polarization surface enhanced NMR spectroscopy being especially helpful by providing enhanced intensities of the signals of H-1, C-13, Si-29, and N-15 at their natural abundances. The characterization data provide details of the synthesis chemistry, including evidence of (a) O-2 insertion into Ta-CH3 species on the support and (b) a binuclear to mononuclear transformation of species formed from Ta-2(OMe)(10) on the support. A catalytic test reaction, cyclooctene epoxidation, was used to probe the supported nanoparticles, with 30% H2O2 serving as the oxidant. The catalysts gave selectivities up to 98% for the epoxide at conversions as high as 99% with a 3.4 wt% loading of Ta present as TaxNy/TaOxNy.

  19. 2007 Lake County Board of County Commissioners Topographic LiDAR: Lake County, Florida

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This metadata document describes the LiDAR point data in LAS format produced by Kucera covering the project area of Lake County, FL. The data produced is...

  20. Best Manufacturing Practices. Report of Survey Conducted at Stafford County Public Schools, Stafford County, VA

    National Research Council Canada - National Science Library

    1994-01-01

    During the week of August 8, 1994, a Best Manufacturing Practices (BMP) survey was conducted at the Stafford County Public Schools located in Stafford County, Virginia, considered one of the fastest growing counties in the state...

  1. Comprehensive adipocytic and neurogenic tissue microarray analysis of NY-ESO-1 expression - a promising immunotherapy target in malignant peripheral nerve sheath tumor and liposarcoma.

    Science.gov (United States)

    Shurell, Elizabeth; Vergara-Lluri, Maria E; Li, Yunfeng; Crompton, Joseph G; Singh, Arun; Bernthal, Nicholas; Wu, Hong; Eilber, Fritz C; Dry, Sarah M

    2016-11-08

    Immunotherapy targeting cancer-testis antigen NY-ESO-1 shows promise for tumors with poor response to chemoradiation. Malignant peripheral nerve sheath tumors (MPNSTs) and liposarcomas (LPS) are chemoresistant and have few effective treatment options. Materials Methods: Using a comprehensive tissue microarray (TMA) of both benign and malignant tumors in primary, recurrent, and metastatic samples, we examined NY-ESO-1 expression in peripheral nerve sheath tumor (PNST) and adipocytic tumors. The PNST TMA included 42 MPNSTs (spontaneous n = 26, NF1-associated n = 16), 35 neurofibromas (spontaneous n = 22, NF-1 associated n = 13), 11 schwannomas, and 18 normal nerves. The LPS TMA included 48 well-differentiated/dedifferentiated (WD/DD) LPS, 13 myxoid/round cell LPS, 3 pleomorphic LPS, 8 lipomas, 1 myelolipoma, and 3 normal adipocytic tissue samples. Stained in triplicate, NY-ESO-1 intensity and density were scored. NY-ESO-1 expression was exclusive to malignant tumors. 100% of myxoid/round cell LPS demonstrated NY-ESO-1 expression, while only 6% of WD/DD LPS showed protein expression, one of which was WD LPS. Of MPNST, 4/26 (15%) spontaneous and 2/16 (12%) NF1-associated MPNSTs demonstrated NY-ESO-1 expression. Strong NY-ESO-1 expression was observed in myxoid/round cell and dedifferentiated LPS, and MPNST in primary, neoadjuvant, and metastatic settings. We found higher prevalence of NY-ESO-1 expression in MPNSTs than previously reported, highlighting a subset of MPNST patients who may benefit from immunotherapy. This study expands our understanding of NY-ESO-1 in WD/DD LPS and is the first demonstration of staining in a WD LPS and metastatic/recurrent myxoid/round cell LPS. These results suggest immunotherapy targeting NY-ESO-1 may benefit patients with aggressive tumors resistant to conventional therapy.

  2. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Science.gov (United States)

    Klar, Agnes S; Gopinadh, Jakka; Kleber, Sascha; Wadle, Andreas; Renner, Christoph

    2015-01-01

    NY-ESO-1 belongs to the cancer/testis antigen (CTA) family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC). We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165) peptide specific chimeric antigen receptor (CAR) CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels. These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  3. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Directory of Open Access Journals (Sweden)

    Agnes S Klar

    Full Text Available NY-ESO-1 belongs to the cancer/testis antigen (CTA family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC.We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165 peptide specific chimeric antigen receptor (CAR CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels.These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  4. Composition and sources of winter and summertime aerosols at Ny Alesund, Spitsbergen

    International Nuclear Information System (INIS)

    Maenhaut, W.; Cornille, P.; Pacyna, J.M.

    1991-01-01

    Filter samples of < 2.5 μm aerosol were collected in (late) winter of 1983, 1984, 1986, and 1987 and in the summer of 1984, 1986, and 1987 at Ny Alesund, Spitsbergen, and analyzed for over 40 elements by a combination of INAA and PIXE. The data sets of the various sampling campaigns and the combined winter and combined summer data were examined by receptor modeling, including absolute principal component analysis (APCA), chemical mass balance (CMB) and multiple linear regression (MLR) techniques. APCA yielded four components, both for the winter and for the summer aerosol. For the winter aerosol, the components were identified as a general pollution component, crustal dust, sea-salt, and a halogen (Br,I) component. The CMB and MLR calculations were used to obtain source (source region) apportionments for the anthropogenic trace elements and for sulfate. For the summer, about 50% of the sulfate was attributed to a marine biogenic source

  5. Chemical and geochemical composition of spring-summer Arctic aerosol collected at Ny Alesund, Svalbard Islands.

    Science.gov (United States)

    Udisti, Roberto; Becagli, Silvia; Caiazzo, Laura; Cappelletti, David; Giardi, Fabio; Grotti, Marco; Lucarelli, Franco; Moroni, Beatrice; Nava, Silvia; Severi, Mirko; Traversi, Rita

    2017-04-01

    Since March 2010, spring-summer (usually March - September) campaigns were continuously carried out at the Italian Gruvebadet Observatory, Ny Alesund, Svalbard Island. Aerosol was sampled by PM10 (daily) and 4-stage (4-day resolution) collector devices and size distribution was evaluated at 10 min resolution in the range 10 nm - 20 um (106 size classes by a TSI SMPS-APS integrated system). Six-year (2010-2015) PM10 and size-segregated (>10, 10-2.5, 2.5-1, metal content (major and trace metals, including Rare Earth Elements - REEs, by PIXE and ICP-MS), Pb isotopic composition (by ICP-MS) and Elemental and Organic Carbon (EC-OC) concentrations. The data set was elaborated by multi-parametric statistical analysis (Positive Matrix Factorization - PMF), in order to identifying and quantifying the contribution of the main anthropic and natural aerosol sources. Particular attention was spent in evaluating the anthropic contribution of nss-sulphate, nitrate, EC and heavy metals during the Arctic Haze in spring. The isotopic composition of Pb was used in identifying the source areas (North America, Greenland, North Europe, Siberia, Iceland) of anthropic emissions as a function of seasonality (different atmospheric circulation pathway). Crustal metals and, especially, REEs anomalies (with respect to the Chondrite-normalized profile) allowed characterizing the dust emissions from their Potential Source Areas (PSA). Biogenic markers (especially methane sulfonic acid - MSA - and bio-nss-sulphate) was used to obtain relevant information about the relationship between marine biogenic activity (primary productivity) and sea ice coverage and atmospheric conditions (irradiance, temperature, circulation pathways). The seasonal pattern of the nitrate deposition was also investigated. Chemical and geochemical measurements were compared with high-resolution size distribution and back-trajectory cluster analysis in order to understand the seasonal pattern of the contributions of long

  6. Antibiotic usage in 2013 on a dairy CAFO in NY State, USA

    Directory of Open Access Journals (Sweden)

    Marie Doane

    2014-05-01

    Full Text Available Antimicrobial resistance is threatening humans and animals worldwide. Biosecurity and 1-year usage of antibiotics on a dairy concentrated animal feeding operation (CAFO in NY State, USA, were mapped: how much antibiotics were used, for what purpose, and whether any decrease could be warranted. Approximately 493 kg antibiotics was used, of which 376 kg was ionophores (monensin and lasalocides, 79 kg penicillin, 16.5 kg lincosamides, 8.0 kg aminoglycosides, 7.7 kg sulfamides, 3.4 kg cephalosporin, 2 kg macrolides, 0.7 kg amphenicols, and 0.1 kg fluoroquinolones. Usage reduction by 84% was realistic without compromising the animal welfare. Further reduction could be possible by improving the biosecurity and by utilizing antibiotic sensitivity testing.

  7. QUEST-lærere fra Holstebro får ny naturfags”trailer” i spil

    DEFF Research Database (Denmark)

    Lysgaard, Troels; Sørensen, Allan; Nielsen, Birgitte Lund

    Ungdomsskolen i Holstebro fik mulighed for at udstyre en ny naturfagstrailer til brug for kommunens naturfagslærere. I den forbindelse var det oplagt at anvende netværket fra to år i QUEST projektet. Derfor har naturfagslærere på efteruddannelse i QUEST målsat, designet og afprøvet undervisningsi...

  8. 33 CFR 100.121 - Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport, Bridgeport, CT. 100.121 Section 100.121 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY REGATTAS AND MARINE PARADES SAFETY OF LIFE ON NAVIGABLE WATERS § 100.121 Swim...

  9. 75 FR 13131 - Notice of Intent To Prepare an Environmental Impact Statement (EIS) for the Sale of Plum Island, NY

    Science.gov (United States)

    2010-03-18

    ... the anticipated sale of Plum Island, New York and an ancillary support facility at Orient Point, New...) for the Sale of Plum Island, NY AGENCY: Office of Real Property Utilization & Disposal; General...: Plum Island is an 840-acre island located approximately 1.5 miles off the northeast tip of Orient Point...

  10. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... floating things in the canal shall be under the direction of the authorized agents of the District Engineer... Bridge. Tugs without tows, tugs towing a single barge under 150 feet in length, and single vessels under... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry Street...

  11. MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases.

    Science.gov (United States)

    Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy; Park, Tristen; Patel, Krishna; Groh, Eric; Rosenberg, Steven A; Miettinen, Markku M

    2016-05-01

    Two cancer testis antigens, the New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and the melanoma-antigen family A (MAGE-A), represent promising immunotherapy targets due to the low expression of these antigens in nonmalignant tissue. To assess overexpression patterns in various cancers, we performed a systematic immunohistochemical analysis for NY-ESO-1 and MAGE-A on tissue array samples of 3668 common epithelial carcinomas (CA) and germ cell tumors of high prevalence and mortality. Here, we find significantly higher expression of MAGE-A (>50% on tumor cells) compared with NY-ESO-1 in several CAs including cutaneous squamous cell carcinomas (SCC) (52.8%/2.8%), esophageal SCC (50%/0%), head and neck SCC (41.1%/ESO-1, was seen in whole slide evaluations of an independent cohort of metastatic SCC (45.5%/3.6%) and metastatic CA (13.5%/0%) of various primaries with significantly higher expression of MAGE-A in metastatic SCC compared with other metastatic CA. MAGE-A is also more highly expressed in germ cell tumors, seminomas (69%/3.5%) and nonseminomas (40.1%/4.7%). In summary, MAGE-A is more highly expressed than NY-ESO-1 in a majority of human malignancies, and targeting MAGE-A may benefit a large number of patients.

  12. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    DEFF Research Database (Denmark)

    Andersen, Lars Dyrskjøt; Zieger, K; Kissow Lildal, T

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse...... the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed...... PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter...

  13. 77 FR 13263 - Foreign-Trade Zone 109 - Watertown, NY; Amendment to Application; North American Tapes, LLC...

    Science.gov (United States)

    2012-03-06

    ... used to produce pressure-sensitive adhesive athletic tape with textile fabric backing material for the... American Tapes, LLC (Textile Athletic Tape), Watertown, NY A request has been submitted to the Foreign... of FTZ 109, to amend its application requesting authority on behalf of North American Tapes (NAT), to...

  14. 77 FR 65015 - Notice of Intent To Repatriate Cultural Items: American Museum of Natural History, New York, NY...

    Science.gov (United States)

    2012-10-24

    ... National Park Service Notice of Intent To Repatriate Cultural Items: American Museum of Natural History... Resources, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024... after that date if no additional claimants come forward. The American Museum of Natural History is...

  15. En ny historie om guld og svovl - guld-svovlbindingen i thiolers adsorption på guldnanopartikler og plane guldoverflader

    DEFF Research Database (Denmark)

    Chi, Qijin; Ford, Michael J.; Halder, Arnab

    2016-01-01

    En netop publiceret større australsk-dansk undersøgelse giver ny indsigt i, hvordan thioldækkede Au-overflader er opbygget. Det har betydning for design, syntese og dannelsesmekanismer for Au-nanopartikler og Au-overflader med skræddersyede fysiske og kemiske egenskaber....

  16. Nineteen-year trends in incidence and indications for laparoscopic cholecystectomy: the NY State experience.

    Science.gov (United States)

    Alli, Vamsi V; Yang, Jie; Xu, Jianjin; Bates, Andrew T; Pryor, Aurora D; Talamini, Mark A; Telem, Dana A

    2017-04-01

    Since the introduction of laparoscopic cholecystectomy (LC), there has been continued evolution in technique, instrumentation and postoperative management. With increased experience, LC has migrated to the outpatient setting. We asked whether increased availability and experience has impacted incidence of and indications for LC. The New York (NY) State Planning and Research Cooperative System longitudinal administrative database was utilized to identify patients who underwent cholecystectomy between 1995 and 2013. ICD-9 and CPT procedure codes were extracted corresponding to laparoscopic and open cholecystectomy and the associated primary diagnostic codes. Data were analyzed as relative change in incidence (normalized to 1000 LC patients) for respective diagnoses. From 1995 to 2013, 711,406 cholecystectomies were performed in NY State: 637,308 (89.58 %) laparoscopic. The overall frequency of cholecystectomy did not increase (1.23 % increase with a commensurate population increase of 6.32 %). Indications for LC during this time were: 72.81 % for calculous cholecystitis (n = 464,032), 4.88 % for biliary colic (n = 31,124), 8.98 % for acalculous cholecystitis (n = 57,205), 3.01 % for gallstone pancreatitis (n = 19,193), and 1.59 % for biliary dyskinesia (n = 10,110). The incidence of calculous cholecystitis declined (-20.09 %, p 320-fold. Analysis of LC through 2014 revealed increasing rates of digestive, infectious, respiratory, and renal complications, with overall cholecystectomy complication rates of 9.29 %. A shifting distribution of operative indications and increasing rates of complications should prompt careful consideration prior to surgery for benign biliary disease. For what is a common procedure, LC carries substantial risk of complications, thus requiring the patient to be an active participant and to share in the decision-making process.

  17. Cancer-testis antigens PRAME and NY-ESO-1 correlate with tumour grade and poor prognosis in myxoid liposarcoma.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Hotokebuchi, Yuka; Ishii, Takeaki; Maekawa, Akira; Yamada, Yuichi; Yamamoto, Hidetaka; Iwamoto, Yukihide; Oda, Yoshinao

    2015-07-01

    Myxoid liposarcoma is the second most common liposarcoma. Although myxoid liposarcoma is relatively chemosensitive and thus a good candidate for chemotherapy, cases with relapsed or metastatic disease still have poor outcome. Here, we performed a gene microarray analysis to compare the gene expression profiles in six clinical myxoid liposarcoma samples and three normal adipose tissue samples, and to identify molecular biomarkers that would be useful as diagnostic markers or treatment targets in myxoid liposarcoma. This showed that the cancer-testis antigen PRAME was up-regulated in myxoid liposarcoma. We then performed immunohistochemical, western blotting and real-time polymerase chain reaction analyses to quantify the expression of PRAME and another cancer-testis antigen, NY-ESO-1, in clinical samples of myxoid liposarcoma (n = 93), dedifferentiated (n = 46), well-differentiated (n = 32) and pleomorphic liposarcomas (n = 14). Immunohistochemically, positivity for PRAME and NY-ESO-1 was observed in 84/93 (90%) and 83/93 (89%) of the myxoid liposarcomas, and in 20/46 (43%) and 3/46 (7%) of the dedifferentiated, 3/32 (9%) and 1/32 (3%) of the well-differentiated and 7/14 (50%) and 3/21 (21%) of the pleomorphic liposarcomas, respectively. High immunohistochemical expression of PRAME and/or NY-ESO-1 was significantly correlated with tumour diameter, the existence of tumour necrosis, a round-cell component of >5%, higher histological grade and advanced clinical stage. High PRAME and NY-ESO-1 expression correlated significantly with poor prognosis in a univariate analysis. The myxoid liposarcomas showed significantly higher protein and mRNA expression levels of PRAME and NY-ESO-1 (CTAG1B) than the other liposarcomas. In conclusion, PRAME and NY-ESO-1 (CTAG1B) were expressed in the vast majority of myxoid liposarcomas, and their high-level expression correlated with tumour grade and poor prognosis. Our results support the potential use of PRAME and NY

  18. Phase II trial of ipilimumab in melanoma patients with preexisting humoural immune response to NY-ESO-1.

    Science.gov (United States)

    Haag, G M; Zoernig, I; Hassel, J C; Halama, N; Dick, J; Lang, N; Podola, L; Funk, J; Ziegelmeier, C; Juenger, S; Bucur, M; Umansky, L; Falk, C S; Freitag, A; Karapanagiotou-Schenkel, I; Beckhove, P; Enk, A; Jaeger, D

    2018-02-01

    Immune checkpoint therapy has dramatically changed treatment options in patients with metastatic melanoma. However, a relevant part of patients still does not respond to treatment. Data regarding the prognostic or predictive significance of preexisting immune responses against tumour antigens are conflicting. Retrospective data suggested a higher clinical benefit of ipilimumab in melanoma patients with preexisting NY-ESO-1-specific immunity. Twenty-five patients with previously untreated or treated metastatic melanoma and preexisting humoural immune response against NY-ESO-1 received ipilimumab at a dose of 10 mg/kg in week 1, 4, 7, 10 followed by 3-month maintenance treatment for a maximum of 48 weeks. Primary endpoint was the disease control rate (irCR, irPR or irSD) according to immune-related response criteria (irRC). Secondary endpoints included the disease control rate according to RECIST criteria, progression-free survival and overall survival (OS). Humoural and cellular immune responses against NY-ESO-1 were analysed from blood samples. Disease control rate according to irRC was 52%, irPR was observed in 36% of patients. Progression-free survival according to irRC was 7.8 months, according to RECIST criteria it was 2.9 months. Median OS was 22.7 months; the corresponding 1-year survival rate was 66.8%. Treatment-related grade 3 AEs occurred in 36% with no grade 4-5 AEs. No clear association was found between the presence of NY-ESO-1-specific cellular or humoural immune responses and clinical activity. Ipilimumab demonstrated clinically relevant activity within this biomarker-defined population. NY-ESO-1 positivity, as a surrogate for a preexisting immune response against tumour antigens, might help identifying patients with a superior outcome from immune checkpoint blockade. NCT01216696. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Immunoediting and persistence of antigen-specific immunity in patients who have previously been vaccinated with NY-ESO-1 protein formulated in ISCOMATRIX™.

    Science.gov (United States)

    Nicholaou, Theo; Chen, Weisan; Davis, Ian D; Jackson, Heather M; Dimopoulos, Nektaria; Barrow, Catherine; Browning, Judy; Macgregor, Duncan; Williams, David; Hopkins, Wendie; Maraskovsky, Eugene; Venhaus, Ralph; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Cebon, Jonathan

    2011-11-01

    NY-ESO-1 protein formulated in ISCOMATRIX™ results in CD4+, CD8+ T cell and antibody-mediated immunity. We evaluated persistence of immunity, relapse-free survival and tumour antigen expression upon relapse in patients vaccinated in an earlier trial. Immunity was measured in 28 patients with resected NY-ESO-1-expressing tumours (melanoma 25, breast 3) 252-1,155 days (median = 681) after vaccination. In the earlier vaccination, trial patients received NY-ESO-1 with ISCOMATRIX™ adjuvant at three protein doses 10 μg, 30 μg or 100 μg (n = 14); 100 μg NY-ESO-1 protein (n = 8) or placebo (n = 6), together with 1 μg of intradermal (ID) NY-ESO-1 protein twice for DTH skin testing. Immune responses assessed in the current study included antibody titres, circulating NY-ESO-1-specific T cells and DTH reactivity 2 days after DTH skin testing with NY-ESO-1 protein (1 μg) or peptides (10 μg). Relapse-free survival was determined for 42 melanoma patients. On relapse NY-ESO-1 and HLA, class I was assessed by immunohistochemistry in 17. Persisting anti-NY-ESO-1 immunity was detected in 10/14 recipients who had previously received vaccine with ISCOMATRIX™ adjuvant. In contrast, immunity only persisted in 3/14 who received 100 μg un-adjuvanted NY-ESO-1 protein (3/8) or 2 μg DTH protein (0/6) P = 0.02. Hence, persisting NY-ESO-1 immunity was associated with prior adjuvant. Tumour NY-ESO-1 or HLA class I was downregulated in participants who relapsed suggesting immunoediting had occurred. Immunoediting suggests that a signal of anti-tumour activity was observed in high-risk resected melanoma patients vaccinated with NY-ESO-1/ISCOMATRIX™. This was associated with measurable persisting immunity in the majority of vaccinated subjects tested. A prospective randomised trial has been undertaken to confirm these results.

  20. Hazardous Waste Cleanup: Consolidated Edison Company of NY - Indian Point 2 in Buchanan, New York

    Science.gov (United States)

    Indian Point Unit 2 is a nuclear-powered electricity generating plant located in the village of Buchanan, Westchester County, New York. The facility generates and stores ignitable and mixed hazardous and radioactive waste on site. On February 28, 1997, New

  1. Geothermal development plan: Maricopa county

    Energy Technology Data Exchange (ETDEWEB)

    White, D.H.

    1981-01-01

    Maricopa county is the area of Arizona receiving top priority since it contains over half of the state's population. The county is located entirely within the Basin and Range physiographic region in which geothermal resources are known to occur. Several approaches were taken to match potential users to geothermal resources. One approach involved matching some of the largest facilities in the county to nearby geothermal resources. Other approaches involved identifying industrial processes whose heat requirements are less than the average assessed geothermal reservoir temperature of 110/sup 0/C (230/sup 0/F). Since many of the industries are located on or near geothermal resources, geothermal energy potentially could be adapted to many industrial processes.

  2. Expression of NY-ESO-1 in Triple-Negative Breast Cancer Is Associated with Tumor-Infiltrating Lymphocytes and a Good Prognosis.

    Science.gov (United States)

    Lee, Hee Jin; Kim, Joo Young; Song, In Hye; Park, In Ah; Yu, Jong Han; Gong, Gyungyub

    2015-01-01

    Accumulating evidence suggests that immunotherapy has great potential for treating triple-negative breast cancer (TNBC). We analyzed the expression of NY-ESO-1, which is a potent immunogenic cancer testis antigen, and its association with clinicopathological factors in large cohorts of breast cancer patients. A total of 623 consecutive breast cancer patients who underwent surgery between 1993 and 1998 and 612 TNBC patients who underwent surgery between 2004 and 2010 at Asan Medical Center were included. Immunohistochemical staining for NY-ESO-1 was performed using tissue microarrays. NY-ESO-1 was expressed in 2.6% of consecutive breast cancers, all of which were TNBC (p ESO-1 expression was identified in 9.7% of the TNBC cohort and was significantly correlated with a higher level of tumor-infiltrating lymphocytes (TIL; p = 0.026). In survival analyses, a lower level of TIL (all, p ESO-1 expression (p = 0.024) were significantly associated with poor disease-free survival. Additionally, positive NY-ESO-1 expression was an independent favorable prognostic factor in TNBC patients (p = 0.046). NY-ESO-1 is specifically expressed in TNBC, and NY-ESO-1 expression is an independent good prognostic factor in TNBC. Evaluation of NY-ESO-1 expression in TNBC might be useful for selecting patients who may benefit from vaccination therapy and also has a prognostic significance in TNBC. © 2015 S. Karger AG, Basel.

  3. Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein.

    Science.gov (United States)

    Komatsu, Nobukazu; Jackson, Heather M; Chan, Kok-fei; Oveissi, Sara; Cebon, Jonathan; Itoh, Kyogo; Chen, Weisan

    2013-07-01

    The tumor antigen NY-ESO-1 is one of the most antigenic cancer-testis antigens, first identified by serologic analysis of a recombinant cDNA expression library (SEREX). NY-ESO-1 is expressed in different types of cancers including melanoma. NY-ESO-1-specific spontaneous humoral and cellular immune responses are detected in a large proportion of patients with advanced NY-ESO-1-expressing cancers. Therefore NY-ESO-1 is a good candidate antigen for immunotherapy. Although cellular immune responses to NY-ESO-1 are well characterized, much less is known about the humoral immune responses. In this study, we finely mapped linear antibody epitopes using sera from melanoma patients and shorter overlapping peptide sets. We have shown that melanoma patients' humoral immune systems responded to NY-ESO-1 differently in each individual with widely differing antibody specificity, intensity and antibody subtypes. This knowledge will help us further understand anti-tumor immunity and may also help us to monitor cancer progress and cancer vaccine efficacy in the future. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. State of Aging in Allegheny County Survey

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — For more than three decades UCSUR has documented the status of older adults in the County along multiple life domains. Every decade we issue a comprehensive report...

  5. 2010 South Carolina DNR Lidar: Saluda County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Provide high density LiDAR elevation data map of Saluda County, SC. Provide Bare Earth DEM (vegetation removal) of Saluda County, SC.

  6. 2010 ARRA Lidar: Bamberg County (SC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Provide high density LiDAR elevation data map of Bamberg County, SC. Provide Bare Earth DEM (vegetation removal) of Bamberg County, SC.

  7. 2010 ARRA Lidar: Hampton County (SC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Provide high density LiDAR elevation data map of Hampton County, SC. Provide Bare Earth DEM (vegetation removal) of Hampton County, SC.

  8. Allegheny County Polling Place Locations (November 2017)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of the polling places in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  9. Allegheny County Addressing StreetDictionary

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the road centerlines in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  10. Allegheny County-Owned Bridges Points

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the location of bridges owned by Allegheny County as centroids. If viewing this description on the Western Pennsylvania Regional Data Center’s...

  11. Allegheny County-Owned Roads Centerlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the roads owned by Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  12. Allegheny County Polling Place Locations (November 2015)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of the polling places in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  13. Allegheny County Pennsylvania Senate District Boundaries

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset demarcates the Pennsylvania Senate district boundaries within Allegheny County. If viewing this description on the Western Pennsylvania Regional Data...

  14. Allegheny County Voting District (2015) Web Map

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This webmap demarcates municipal voting districts in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  15. Allegheny County Polling Place Locations (November 2014)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of the polling places in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  16. Allegheny County Weights and Measures Inspections

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Inspections conducted by the Allegheny County Bureau of Weights and Measures. The Bureau inspects weighing and timing devices such as gas pumps, laundromat timers,...

  17. Port Authority of Allegheny County Transit Stops

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — All transit stops within the Port Authority of Allegheny County's service area for the November 20, 2016 - March (TBD) 2017 schedule period.

  18. Allegheny County Polling Place Locations (November 2016)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of the polling places in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  19. Douglas County Historical Rectified Aerial Photos 1954

    Data.gov (United States)

    Kansas Data Access and Support Center — This raster dataset consists of approximately 200 aerial photographs taken in 1954 in Douglas county, Kansas, United States. The Douglas County Public Works...

  20. Allegheny County Particulate Matter 2.5

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The U.S. Environmental Protection Agency provides information on the particulate matter concentration for Allegheny County that have a diameter greater or equal to...

  1. Allegheny County-Owned Bridges Centerlines

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the bridges owned by Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  2. Elevation - LIDAR Survey - Roseau County, Minnesota

    Data.gov (United States)

    Army Corps of Engineers, Department of the Army, Department of Defense — LIDAR Data for Roseau County Minnesota. This project consists of approximately 87 square miles of LIDAR mapping in Roseau County, Minnesota at two sites: area 1,...

  3. Allegheny County Magisterial Districts Outlines (2015)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the magisterial districts in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data portal...

  4. Douglas County Historical Rectified Aerial Photos 1937

    Data.gov (United States)

    Kansas Data Access and Support Center — This raster dataset consists of approximately 200 aerial photographs taken in 1937 in Douglas county, Kansas, United States. The Douglas County Public Works...

  5. Allegheny County Voting District (2016) Web Map

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This webmap demarcates municipal voting districts in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  6. Allegheny County Median Age at Death

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The median age at death is calculated for each municipality in Allegheny County. Data is based on the decedent's residence at the time of death, not the location...

  7. Allegheny County Polling Place Locations (May 2017)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset contains locations of the polling places in Allegheny County.If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  8. Allegheny County Farmers Markets Locations (2017)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This dataset shows the locations of farmers markets in Allegheny County. If viewing this description on the Western Pennsylvania Regional Data Center’s open data...

  9. Address Points - Allegheny County Address Points 201601

    Data.gov (United States)

    NSGIC Education | GIS Inventory — This dataset contains Address Points in Allegheny County. The Address Points were created by GDR for the Allegheny County CAD project, October 2008. Data is updated...

  10. Uplift history of the Villány Hills (SW Hungary) based on paleontological data

    Science.gov (United States)

    Sebe, Krisztina; Pazonyi, Piroska; Gasparik, Mihály; Lajos Szujó, Gábor

    2015-04-01

    Villány Hills are the southernmost basement outcrop in Hungary, along the northern margin of the Drava Basin. They comprise part of a Cretaceous nappe in the Tisza structural unit. Data on their young tectonic evolution are scarce, mostly due to the almost complete lack of Cenozoic sediments. Though somewhat debated, indirect data suggest that most of the area was covered by Upper Miocene (Lake Pannon) clastic sediments. Uplift and exhumation of the basement was driven by Late Miocene to post-Miocene basin inversion. A unique opportunity to study the uplift history is offered by paleontological data, by small but numerous terrestrial vertebrate sites, which cover a long time span in the Pliocene and Pleistocene. Faunas accumulated in fissure fillings of karstified limestone and thus provide a minimum age for the subaerial exposure (exhumation) of the bedrock at the given site. For some sites it has been proposed that water-linked species in the fauna refer to the existence of an alluvial plain on the clastic cover, in immediate proximity to the bedrock. If true, this would give an exact elevation of the surrounding terrain at the time indicated by the fauna. If exhumation of the basement happened during the time interval covered by the vertebrate faunas, then a relationship is expected to exist between the age and elevation of the the fossil sites, with the youngest faunas lying at lowest elevation. This relationship is expected to be strictly valid primarily in the case of water-linket faunas, but as a trend also for all fossil sites. We collected the age data and ecological requirements of the reliably datable vertebrate faunas and their locations with the possible precision. Then the relationship between the elevation and age was studied and compared to the available geomorphological, sedimentological and tectonic data. The age-elevation distribution of vertebrate faunas did not show the expected relationship. Based on the results, exhumation of the Villány

  11. NY-ESO-1 Vaccination in Combination with Decitabine Induces Antigen-Specific T-lymphocyte Responses in Patients with Myelodysplastic Syndrome.

    Science.gov (United States)

    Griffiths, Elizabeth A; Srivastava, Pragya; Matsuzaki, Junko; Brumberger, Zachary; Wang, Eunice S; Kocent, Justin; Miller, Austin; Roloff, Gregory W; Wong, Hong Yuen; Paluch, Benjamin E; Lutgen-Dunckley, Linda G; Martens, Brandon L; Odunsi, Kunle; Karpf, Adam R; Hourigan, Christopher S; Nemeth, Michael J

    2017-09-25

    Purpose: Treatment options are limited for patients with high-risk myelodysplastic syndrome (MDS). The azanucleosides, azacitidine and decitabine, are first-line therapy for MDS that induce promoter demethylation and gene expression of the highly immunogenic tumor antigen NY-ESO-1. We demonstrated that patients with acute myeloid leukemia (AML) receiving decitabine exhibit induction of NY-ESO-1 expression in circulating blasts. We hypothesized that vaccinating against NY-ESO-1 in patients with MDS receiving decitabine would capitalize upon induced NY-ESO-1 expression in malignant myeloid cells to provoke an NY-ESO-1-specific MDS-directed cytotoxic T-cell immune response. Experimental Design: In a phase I study, 9 patients with MDS received an HLA-unrestricted NY-ESO-1 vaccine (CDX-1401 + poly-ICLC) in a nonoverlapping schedule every four weeks with standard-dose decitabine. Results: Analysis of samples serially obtained from the 7 patients who reached the end of the study demonstrated induction of NY-ESO-1 expression in 7 of 7 patients and NY-ESO-1-specific CD4 + and CD8 + T-lymphocyte responses in 6 of 7 and 4 of 7 of the vaccinated patients, respectively. Myeloid cells expressing NY-ESO-1, isolated from a patient at different time points during decitabine therapy, were capable of activating a cytotoxic response from autologous NY-ESO-1-specific T lymphocytes. Vaccine responses were associated with a detectable population of CD141 Hi conventional dendritic cells, which are critical for the uptake of NY-ESO-1 vaccine and have a recognized role in antitumor immune responses. Conclusions: These data indicate that vaccination against induced NY-ESO-1 expression can produce an antigen-specific immune response in a relatively nonimmunogenic myeloid cancer and highlight the potential for induced antigen-directed immunotherapy in a group of patients with limited options. Clin Cancer Res; 24(5); 1-11. ©2017 AACR. See related commentary by Fuchs, p. 991 . ©2017 American

  12. The cancer-testis antigen NY-ESO-1 is highly expressed in myxoid and round cell subset of liposarcomas.

    Science.gov (United States)

    Hemminger, Jessica A; Ewart Toland, Amanda; Scharschmidt, Thomas J; Mayerson, Joel L; Kraybill, William G; Guttridge, Denis C; Iwenofu, O Hans

    2013-02-01

    Liposarcomas are a heterogenous group of fat-derived sarcomas, and surgery with or without chemoradiation therapy remains the main stay of treatment. NY-ESO-1 is a cancer-testis antigen expressed in various cancers where it can induce both cellular and humoral immunity. Immunotherapy has shown promise in clinical trials involving NY-ESO-1-expressing tumors. Gene expression studies have shown upregulation of the gene for NY-ESO-1, CTAG1B, in myxoid and round cell liposarcomas. Herein, we evaluated the expression of NY-ESO-1 among liposarcoma subtypes by quantitative real-time PCR, western blot analysis, and immunohistochemistry. Frozen tissue for quantitative real-time PCR and western blot analysis was obtained for the following liposarcoma subtypes (n=15): myxoid and round cell (n=8); well-differentiated (n=4), and dedifferentiated (n=3). Formalin-fixed paraffin-embedded blocks were obtained for the following liposarcoma subtypes (n=44): myxoid and round cell (n=18); well-differentiated (n=10); dedifferentiated (n=10); and pleomorphic (n=6). Full sections were stained with monoclonal antibody NY-ESO-1, and staining was assessed for intensity (1-3+), percentage of tumor positivity, and location. In all, 7/8 (88%) and 16/18 (89%) myxoid and round cell expressed CTAG1B and NY-ESO-1 by quantitative real-time PCR and immunohistochemistry, respectively. Western blot correlated with mRNA expression levels. By immunohistochemistry, 94% (15/16) of positive cases stained homogenously with 2-3+ intensity. Also, 3/6 (50%) pleomorphic liposarcomas demonstrated a range of staining: 1+ intensity in 50% of cells; 2+ intensity in 5% of cells; and 3+ intensity in 90% of cells. One case of dedifferentiated liposarcoma showed strong, diffuse staining (3+ intensity in 75% of cells). Our study shows that both CTAG1B mRNA and protein are overexpressed with high frequency in myxoid and round cell liposarcoma, enabling the potential use of targeted immunotherapy in the treatment of this

  13. Digitalisering i offentlig sektor : digital ledelse som ny ledelsesdisiplin : hva hemmer og fremmer god digital ledelse?

    OpenAIRE

    Fjørtoft, Siw Olsen

    2014-01-01

    This master thesis originates from my interest in new technology and the digitization processes that we see in society today. Due to my background in the field of education, I have both learned from and experienced many of the opportunities that go with digitization. However, I have also witnessed large variations in the public sector when it comes to the implementation and practice of these processes. The government has ordered public reports and written White Papers. Counties and municipali...

  14. National Dam Safety Program. Cobbs Hill Reservoir Dam (Inventory Number NY 1448), Lake Ontario Basin, Monroe County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-06-30

    Reservoir are hori- zontally lying dolostones of the Lockport Group of Upper Silurian age. Depth to bedrock is unknown. The reservoir is sited on Cobbs Hill...t7 oil L PE w !J " ° ~A 92o IuIsI I FIGURE lb Q , ,. IL I .i. *,’X. ’ -,_ OIL (I) ," ’. o S ,.’lt / I .. ’ " I,"_ __4- . . l ’’I -. .* . I- I ’i’.I

  15. National Dam Safety Program. Glenmere Lake Dam, (Inventory Number N.Y. 224), Lower Hudson River Basin, Orange County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-09-14

    the erosion of overlying Silurian and Devonian limestones. Drainage is generally northeast towards the Hudson River. b. Subsurface Investigation Tfhe...17 17M OIL SIDE ( * R Fib ids S T 10 Sf0 STAI, ANN jilt go S LA ugar Loa t 17A WHIT( OAK 10, 94 Wickham % Village I M ills p ke owarv, a SM& ScIlIv

  16. National Dam Safety Program. Mud Lock C&S Canal Dam (Inventory Number N.Y. 416) Oswego River Basin, Cayuga County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-09-14

    the dam is underlain chiefly by Camillus shale of upper Silurian age. Available publications do not indicate the presence of any faulting in the bedrock...In a OD ~ Oil . *W K ~ ~ ~ C tn. ’ m elNl.l~f In fn r.i NMD fn~4 d.Jm’ die .. mD Ln m P.%WN tofC WI O - i.. Cc~ W)oe - u’ o nr on(I I 0, a@, O a, tn N

  17. National Dam Safety Program. Wiscoy Dam (Inventory Number N.Y. 461), Genesee River Basin, Allegheny County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-08-27

    York. Piase I 13. SUPPLEM.TARY NOTE Inspection Report 19. K iY ROS (Conrthw on row... oido if noc. s r md Idnlty by bloc>. rmbe)ID =_ Safety...C3 CL &L. 0 4a4E Il 0 I% 0 E000 C4 co BtD%% E~ T - <. r-,.r.- cc )%N =00 00 C%%% 00 Ito 4, EL - II C> ɘu E v 4a 0 0N0 NI fn 0w 0 * SECTION 6...6-N wtn SjEcT 1 ~ D-1 J’ ~ DATE7~/k ERMAN, ANTHONY, ASSOCIATES 𔃿T~KD DATE " _ _ susJUcT 7/ / - 0 u- TN. Z dc47%/ 4 - I el c6). _-Ts- -1 70 60. -2

  18. National Dam Safety Program. Norwich Water Works Dam Number 1 (Inventory Number NY 347), Susquehanna River Basin, Chenango County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1981-08-04

    explorations were made at the site. Based on reports made in the mid 1920’s, the subsurface conditions at the site consist of relatively impermeable glacial...noode~d soil ip 2O~i ’’. ChC b-, a concreto .nd ru.aonry ruir, or overflow ;ii-*.)£ cr; b47t8011 tw.,o win; walls off t ona und concroto; wii~’ . ~t

  19. National Dam Safety Program. Lake Flower Dam, Inventory Number NY-707. Lake Champlain River Basin. Franklin County, New York. Phase I Inspection Report,

    Science.gov (United States)

    1980-08-01

    33 .835 3,37 135 15.30 2b7 3, 7943 / i3 /.0 /,o-a.3 )/,Z /,33 //. 3 n_ a ) ~~ ~ ~ ~ o 5v-z 3, , , / 2-’Z’ /1 6 .3755" 1536 /o 3,3.3 6-250 • ,,’.,1 4...0x Q 0 (x wI’. . . . . . . . . 2 U)j 1 0 1. U) 4 -n 21)~~ 30IX4 -c I, _j)N r . , 24I -. N Im In 1c 4 .0 ~ A2 * cc. acc C- a LauC tD cz 3 0 V, O aL -o

  20. National Dam Safety Program. Millbrook Watershed Project Site I (inventory Number NY 715) Susquehanna River Basin, Chenango County, New York. Phase 1 Inspection Report

    Science.gov (United States)

    1980-09-30

    Milibrook Watershed Project Site T fn~ %/ V National. Darm Safety Program Albny 1223 6. PJERMN ORG RAE04 NUBE Department of th Ar4 Neworek, No 128 9...4U CA > c~ 9%1 Ul NU.LUr -w Z CC 00 tn U.0D L ft SA. .4 ft 0WO ’ O ~02 cmi at 0 0 LA . 30S-U 30 0 ... J 00 z A ~0 3l zu 0 &u .ft~z ft 0 00 -. Q U. m...m m -4 4( -- jC 1- h .0 m -14 0. ~~Ia U% -4-4 tI L.4ET ID eu - - - - - N CU44-. -4..-4 - -L U SlU 20 uv0. 0. zcc f 0 LL U W LA N 0 - a U~ a. C.cr r L