Differential expression of nanog1 and nanogp8 in colon cancer cells

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Ishiguro, Tatsuya; Sato, Ai; Ohata, Hirokazu; Sakai, Hiroaki [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Nakagama, Hitoshi, E-mail: hnakagam@ncc.go.jp [Division of Cancer Development System, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Okamoto, Koji, E-mail: kojokamo@ncc.go.jo [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)

2012-02-10

Highlights: Black-Right-Pointing-Pointer Nanog is expressed in a majority of colon cancer cell lines examined. Black-Right-Pointing-Pointer Both nanog1 and nanogp8 are expressed in colon cancer cells with varying ratios. Black-Right-Pointing-Pointer Nanog mediates cell proliferation of colon cancer cells. Black-Right-Pointing-Pointer Nanog predominantly localizes in cytoplasm of colon cancer cells. -- Abstract: Nanog, a homeodomain transcription factor, is an essential regulator for promotion of self-renewal of embryonic stem cells and inhibition of their differentiation. It has been demonstrated that nanog1 as well as nanogp8, a retrogene of nanog1, is preferentially expressed in advanced stages of several types of cancer, suggesting their involvement during cancer progression. Here, we investigated the expression of Nanog in well-characterized colon cancer cell lines. Expression of Nanog was detectable in 5 (HCT116, HT29, RKO, SW48, SW620) out of seven cell lines examined. RNA expression analyses of nanog1 and nanogp8 indicated that, while nanog1 was a major form in SW620 as well as in teratoma cells Tera-2, nanogp8 was preferentially expressed in HT29 and HCT116. In accordance with this, shRNA-mediated knockdown of nanog1 caused the reduction of Nanog in SW620 but not in HT29. Inhibition of Nanog in SW620 cells negatively affected cell proliferation and tumor formation in mouse xenograft. Biochemical subcellular fractionation and immunostaining analyses revealed predominant localization of Nanog in cytoplasm in SW620 and HT29, while it was mainly localized in nucleus in Tera-2. Our data indicate that nanog1 and nanogp8 are differentially expressed in colon cancer cells, and suggest that their expression contributes to proliferation of colon cancer cells.

Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions.

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Slamecka, Jaroslav; Salimova, Lilia; McClellan, Steven; van Kelle, Mathieu; Kehl, Debora; Laurini, Javier; Cinelli, Paolo; Owen, Laurie; Hoerstrup, Simon P; Weber, Benedikt

2016-01-01

Amniotic fluid stem cells (AFSC) represent an attractive potential cell source for fetal and pediatric cell-based therapies. However, upgrading them to pluripotency confers refractoriness toward senescence, higher proliferation rate and unlimited differentiation potential. AFSC were observed to rapidly and efficiently reacquire pluripotency which together with their easy recovery makes them an attractive cell source for reprogramming. The reprogramming process as well as the resulting iPSC epigenome could potentially benefit from the unspecialized nature of AFSC. iPSC derived from AFSC also have potential in disease modeling, such as Down syndrome or β-thalassemia. Previous experiments involving AFSC reprogramming have largely relied on integrative vector transgene delivery and undefined serum-containing, feeder-dependent culture. Here, we describe non-integrative oriP/EBNA-1 episomal plasmid-based reprogramming of AFSC into iPSC and culture in fully chemically defined xeno-free conditions represented by vitronectin coating and E8 medium, a system that we found uniquely suited for this purpose. The derived AF-iPSC lines uniformly expressed a set of pluripotency markers Oct3/4, Nanog, Sox2, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 in a pattern typical for human primed PSC. Additionally, the cells formed teratomas, and were deemed pluripotent by PluriTest, a global expression microarray-based in-silico pluripotency assay. However, we found that the PluriTest scores were borderline, indicating a unique pluripotent signature in the defined condition. In the light of potential future clinical translation of iPSC technology, non-integrating reprogramming and chemically defined culture are more acceptable.

Phosphorylation of Nanog is Essential to Regulate Bmi1 and Promote Tumorigenesis

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Xie, Xiujie; Piao, Longzhu; Cavey, Greg S.; Old, Matthew; Teknos, Theodoros N.; Mapp, Anna K; Pan, Quintin

2014-01-01

Emerging evidence indicates that Nanog is intimately involved in tumorigenesis in part through regulation of the cancer initiating cell population. However, the regulation and role of Nanog in tumorigenesis are still poorly understood. In this study, human Nanog was identified to be phosphorylated by human PKCε at multiple residues including T200 and T280. Our work indicated that phosphorylation at T200 and T280 modulates Nanog function through several regulatory mechanisms. Results with phosphorylation-insensitive and phosphorylation-mimetic mutant Nanog revealed that phosphorylation at T200 and T280 enhance Nanog protein stability. Moreover, phosphorylation-insensitive T200A and T280A mutant Nanog had a dominant-negative function to inhibit endogenous Nanog transcriptional activity. Inactivation of Nanog was due to impaired homodimerization, DNA binding, promoter occupancy, and p300, a transcriptional co-activator, recruitment resulting in a defect in target gene promoter activation. Ectopic expression of phosphorylation-insensitive T200A or T280A mutant Nanog reduced cell proliferation, colony formation, invasion, migration, and the cancer initiating cell population in head and neck squamous cell carcinoma (HNSCC) cells. The in vivo cancer initiating ability was severely compromised in HNSCC cells expressing phosphorylation-insensitive T200A or T280A mutant Nanog; 87.5% (14/16), 12.5% (1/8), and 0% (0/8) for control, T200A, and T280A, respectively. Nanog occupied the Bmi1 promoter to directly transactivate and regulate Bmi1. Genetic ablation and rescue experiments demonstrated that Bmi1 is a critical downstream signaling node for the pleiotropic, pro-oncogenic effects of Nanog. Taken together, our study revealed, for the first time, that post-translational phosphorylation of Nanog is essential to regulate Bmi1 and promote tumorigenesis. PMID:23708658

Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.

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Ravindran, Gokulan; Sawant, Sharada S; Hague, Angela; Kingsley, Karl; Devaraj, Halagowder

2015-07-01

The re-expression of pluripotent markers (Oct-4 and Nanog) and the reactivation of stem cell-related pathways in oral carcinoma have been well researched. However, the relationship between the stem cell signaling molecule β-catenin and pluripotent markers Oct-4 and Nanog in oral cancer is yet to be studied in detail. Therefore, we have investigated the correlation among Oct-4, Nanog, and β-catenin in oral squamous cell carcinoma, which, in turn, could provide valuable insight into its prognostic significance. The immunohistochemical analysis was performed for 60 cases of oral cancer to study the expression pattern of Oct-4, Nanog, and β-catenin. Whereas immunofluorescence analysis was used to investigate the co-localization of β-catenin with Oct-4 and Nanog in oral carcinoma tissues and H314 cell line. Finally, co-immunoprecipitation analysis was used to study the possible interaction between β-catenin and Oct-4 in oral carcinoma cells. β-catenin, Oct-4, and Nanog showed significant correlation with lymph node metastasis, stage, grade, and prognosis in oral squamous cell carcinoma. Interestingly, a significant positive correlation was found among the expression of Oct-4, Nanog, and β-catenin. Moreover, the interaction between β-catenin and Oct-4 was observed in oral cancer. The positive correlation among Oct-4, Nanog, and β-catenin suggests their coordinated role in maintaining proliferation in oral carcinoma cells. The interaction between β-catenin and Oct-4 may be a crucial event in oral carcinogenesis. On the other hand, β-catenin, Oct-4, and Nanog could be used as independent prognostic markers of oral squamous cell carcinoma. © 2014 Wiley Periodicals, Inc.

[Prokaryotic expression of Nanog gene and preparation of anti-Nanog antibody].

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Li, Jun; Wang, Xiao-min; Dou, Zhong-ying; Li, Yong

2012-07-01

To express Nanog fusion protein in Escherichia coli ( E.coli), and to prepare rabbit anti-mouse polyclonal antibodies to the Nanog fusion protein. Mouse Nanog gene was amplified from the pNA992 recombinant plasmid and inserted into pET-32a vector to construct a recombinant expression vector pET-32a-Nanog. The recombinant vector was transfected into E.coli BL21 and induced by IPTG to express in them. The acquired Nanog fusion protein was purified with HisTrap affinity column and injected as an antigen into rabbits for preparing polyclonal antibodies. At last, the titer and specificity of the polyclonal antibodies were analyzed with indirect ELISA, Western blotting and immunocytochemical staining, respectively. The recombinant expression vector pET-32a-Nanog was successfully prepared, transfected and induced to obtain the high expression of the Nanog fusion protein in a form of inclusion bodies in E.coli. After purification, its purity was up to 97%. The titer of anti-Nanog antibodies was 1:32 000 in the immunized rabbit serum, and exhibited a high specificity to Nanog protein. The rabbit anti-mouse polyclonal antibodies have been prepared successfully with a high titer and specificity to the Nanog fusion protein.

Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces

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Venable, Alison; Mitalipova, Maisam; Lyons, Ian; Jones, Karen; Shin, Soojung; Pierce, Michael; Stice, Steven

2005-01-01

Background Pluripotent human embryonic stem cells (hESCs) have the potential to form every cell type in the body. These cells must be appropriately characterized prior to differentiation studies or when defining characteristics of the pluripotent state. Some developmentally regulated cell surface antigens identified by monoclonal antibodies in a variety of species and stem cell types have proven to be side chains of membrane glycolipids and glycoproteins. Therefore, to examine hESC surfaces for other potential pluripotent markers, we used a panel of 14 lectins, which were chosen based on their specificity for a variety of carbohydrates and carbohydrate linkages, along with stage specific embryonic antigen-4 (SSEA-4), to determine binding quantitation by flow cytometry and binding localization in adherent colonies by immunocytochemistry. Results Enriching cells for SSEA-4 expression increased the percentage of SSEA-4 positive cells to 98–99%. Using enriched high SSEA-4-expressing hESCs, we then analyzed the binding percentages of selected lectins and found a large variation in binding percentages ranging from 4% to 99% binding. Lycopersicon (tomato)esculetum lectin (TL), Ricinus communis agglutinin (RCA), and Concanavalin A (Con A) bound to SSEA-4 positive regions of hESCs and with similar binding percentages as SSEA-4. In contrast, we found Dolichos biflorus agglutinin (DBA) and Lotus tetragonolobus lectin (LTL) did not bind to hESCs while Phaseolus vulgaris leuco-agglutinin (PHA-L), Vicia villosa agglutinin (VVA), Ulex europaeus agglutinin (UEA), Phaseolus vulgaris erythro-agglutinin (PHA-E), and Maackia amurensis agglutinin (MAA) bound partially to hESCs. These binding percentages correlated well with immunocytochemistry results. Conclusion Our results provide information about types of carbohydrates and carbohydrate linkages found on pluripotent hESC surfaces. We propose that TL, RCA and Con A may be used as markers that are associated with the pluripotent

Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces

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Shin Soojung

2005-07-01

Full Text Available Abstract Background Pluripotent human embryonic stem cells (hESCs have the potential to form every cell type in the body. These cells must be appropriately characterized prior to differentiation studies or when defining characteristics of the pluripotent state. Some developmentally regulated cell surface antigens identified by monoclonal antibodies in a variety of species and stem cell types have proven to be side chains of membrane glycolipids and glycoproteins. Therefore, to examine hESC surfaces for other potential pluripotent markers, we used a panel of 14 lectins, which were chosen based on their specificity for a variety of carbohydrates and carbohydrate linkages, along with stage specific embryonic antigen-4 (SSEA-4, to determine binding quantitation by flow cytometry and binding localization in adherent colonies by immunocytochemistry. Results Enriching cells for SSEA-4 expression increased the percentage of SSEA-4 positive cells to 98–99%. Using enriched high SSEA-4-expressing hESCs, we then analyzed the binding percentages of selected lectins and found a large variation in binding percentages ranging from 4% to 99% binding. Lycopersicon (tomatoesculetum lectin (TL, Ricinus communis agglutinin (RCA, and Concanavalin A (Con A bound to SSEA-4 positive regions of hESCs and with similar binding percentages as SSEA-4. In contrast, we found Dolichos biflorus agglutinin (DBA and Lotus tetragonolobus lectin (LTL did not bind to hESCs while Phaseolus vulgaris leuco-agglutinin (PHA-L, Vicia villosa agglutinin (VVA, Ulex europaeus agglutinin (UEA, Phaseolus vulgaris erythro-agglutinin (PHA-E, and Maackia amurensis agglutinin (MAA bound partially to hESCs. These binding percentages correlated well with immunocytochemistry results. Conclusion Our results provide information about types of carbohydrates and carbohydrate linkages found on pluripotent hESC surfaces. We propose that TL, RCA and Con A may be used as markers that are associated with the

In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133

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Barraud, Perrine; Stott, Simon; Møllgård, Kjeld

2007-01-01

The stage-specific embryonic antigen 4 (SSEA4) is commonly used as a cell surface marker to identify the pluripotent human embryonic stem (ES) cells. Immunohistochemistry on human embryonic central nervous system revealed that SSEA4 is detectable in the early neuroepithelium, and its expression....... Therefore, we propose that SSEA4 associated with CD133 can be used for both the positive selection and the enrichment of neural stem/progenitor cells from human embryonic forebrain....... decreases as development proceeds. Flow cytometry analysis of forebrain-derived cells demonstrated that the SSEA4-expressing cells are enriched in the neural stem/progenitor cell fraction (CD133(+)), but are rarely codetected with the neural stem cell (NSC) marker CD15. Using a sphere-forming assay, we...

CD44 and SSEA-4 positive cells in an oral cancer cell line HSC-4 possess cancer stem-like cell characteristics.

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Noto, Zenko; Yoshida, Toshiko; Okabe, Motonori; Koike, Chika; Fathy, Moustafa; Tsuno, Hiroaki; Tomihara, Kei; Arai, Naoya; Noguchi, Makoto; Nikaido, Toshio

2013-08-01

Cancer may be derived from cancer stem-like cells (CSCs), which are tumor-initiating cells that have properties similar to those of stem cells. Identification and isolation of CSCs needs to be improved further. CSCs markers were examined in human oral cancer cell lines by flow cytometry. The stem cell properties of subpopulations expressing different markers were assessed further by in vitro sphere formation assays, expression of stemness genes, drug resistance assays, and the ability to form tumors in nude mice. We demonstrated that CSCs could be isolated by the cell surface markers CD44 and stage-specific embryonic antigen-4 (SSEA-4). CD44+SSEA-4+ cells exhibited cancer stem-like properties, including extensive self-renewal into the bulk of cancer cells. In vivo xenograft experiments indicated that CD44+SSEA-4+ cells exhibit the highest tumorigenic capacity compared with the remaining subpopulations and parental cells. Double-positive cells for CD44 and SSEA-4 exhibited preferential expression of some stemness genes and were more resistant to the anticancer drugs, cisplatin and 5-fluorouracil (5-FU). In addition, cells expressing CD44 and SSEA-4 were detected in all tumor specimens analyzed, while coexpression of CD44 and SSEA-4 was not detectable in normal oral mucosa. Our findings suggest that CD44+SSEA-4+ cells exhibit the characteristics of CSCs in oral squamous cell carcinoma and provide a target for the development of more effective therapies. Copyright © 2013 Elsevier Ltd. All rights reserved.

Analysis list: Nanog [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Nanog Liver,Pluripotent stem cell + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nano...g.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nanog.5.tsv http://dbarchive.bi...osciencedbc.jp/kyushu-u/mm9/target/Nanog.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Nanog.Li...ver.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Nanog.Pluripotent_ste

Targeting cancer stem cells: emerging role of Nanog transcription factor

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Wang ML

2013-09-01

Full Text Available Mong-Lien Wang,1 Shih-Hwa Chiou,2,3 Cheng-Wen Wu1,4–61Institute of Biochemistry and Molecular Biology, 2Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan; 3Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan; 4Institute of Microbiology and Immunology, 5Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan; 6Institute of Biomedical Science, Academia Sinica, Taipei, TaiwanAbstract: The involvement of stemness factors in cancer initiation and progression has drawn much attention recently, especially after the finding that introducing four stemness factors in somatic cells is able to reprogram the cells back to an embryonic stem cell-like state. Following accumulating data revealing abnormal elevated expression levels of key stemness factors, like Nanog, Oct4, and Sox2, in several types of cancer stem cells; the importance and therapeutic potential of targeting these stemness regulators in cancers has turned to research focus. Nanog determines cell fate in both embryonic and cancer stem cells; activating Nanog at an inappropriate time would result in cancer stem cells rather than normal pluripotent stem cells or differentiated somatic cells. Upregulated Nanog is correlated with poor survival outcome of patients with various types of cancer. The discoveries of downstream regulatory pathways directly or indirectly mediated by Nanog indicate that Nanog regulates several aspects of cancer development such as tumor cell proliferation, self-renewal, motility, epithelial-mesenchymal transition, immune evasion, and drug-resistance, which are all defined features for cancer stem cells. The current review paper illustrates the central role of Nanog in the regulatory networks of cancer malignant development and stemness acquirement, as well as in the communication between cancer cells and the surrounding stroma. Though a more defined model is needed to test the

Human Nanog pseudogene8 promotes the proliferation of gastrointestinal cancer cells

International Nuclear Information System (INIS)

Uchino, Keita; Hirano, Gen; Hirahashi, Minako; Isobe, Taichi; Shirakawa, Tsuyoshi; Kusaba, Hitoshi; Baba, Eishi; Tsuneyoshi, Masazumi; Akashi, Koichi

2012-01-01

There is emerging evidence that human solid tumor cells originate from cancer stem cells (CSCs). In cancer cell lines, tumor-initiating CSCs are mainly found in the side population (SP) that has the capacity to extrude dyes such as Hoechst 33342. We found that Nanog is expressed specifically in SP cells of human gastrointestinal (GI) cancer cells. Nucleotide sequencing revealed that NanogP8 but not Nanog was expressed in GI cancer cells. Transfection of NanogP8 into GI cancer cell lines promoted cell proliferation, while its inhibition by anti-Nanog siRNA suppressed the proliferation. Immunohistochemical staining of primary GI cancer tissues revealed NanogP8 protein to be strongly expressed in 3 out of 60 cases. In these cases, NanogP8 was found especially in an infiltrative part of the tumor, in proliferating cells with Ki67 expression. These data suggest that NanogP8 is involved in GI cancer development in a fraction of patients, in whom it presumably acts by supporting CSC proliferation. -- Highlights: ► Nanog maintains pluripotency by regulating embryonic stem cells differentiation. ► Nanog is expressed in cancer stem cells of human gastrointestinal cancer cells. ► Nucleotide sequencing revealed that Nanog pseudogene8 but not Nanog was expressed. ► Nanog pseudogene8 promotes cancer stem cells proliferation. ► Nanog pseudogene8 is involved in gastrointestinal cancer development.

SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex

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Brøchner, Christian B; Møllgård, Kjeld

2016-01-01

The glycosphingolipid SSEA-4 and the glycoprotein YKL-40 have both been associated with human embryonic and neural stem cell differentiation. We investigated the distribution of SSEA-4 and YKL-40 positive cells in proliferative zones of human fetal forebrain using immunohistochemistry and double-...

Human Nanog pseudogene8 promotes the proliferation of gastrointestinal cancer cells

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Uchino, Keita, E-mail: uchino13@intmed1.med.kyushu-u.ac.jp [Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan); Hirano, Gen [Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan); Hirahashi, Minako [Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka (Japan); Isobe, Taichi; Shirakawa, Tsuyoshi; Kusaba, Hitoshi; Baba, Eishi [Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan); Tsuneyoshi, Masazumi [Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka (Japan); Akashi, Koichi [Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 (Japan)

2012-09-10

There is emerging evidence that human solid tumor cells originate from cancer stem cells (CSCs). In cancer cell lines, tumor-initiating CSCs are mainly found in the side population (SP) that has the capacity to extrude dyes such as Hoechst 33342. We found that Nanog is expressed specifically in SP cells of human gastrointestinal (GI) cancer cells. Nucleotide sequencing revealed that NanogP8 but not Nanog was expressed in GI cancer cells. Transfection of NanogP8 into GI cancer cell lines promoted cell proliferation, while its inhibition by anti-Nanog siRNA suppressed the proliferation. Immunohistochemical staining of primary GI cancer tissues revealed NanogP8 protein to be strongly expressed in 3 out of 60 cases. In these cases, NanogP8 was found especially in an infiltrative part of the tumor, in proliferating cells with Ki67 expression. These data suggest that NanogP8 is involved in GI cancer development in a fraction of patients, in whom it presumably acts by supporting CSC proliferation. -- Highlights: Black-Right-Pointing-Pointer Nanog maintains pluripotency by regulating embryonic stem cells differentiation. Black-Right-Pointing-Pointer Nanog is expressed in cancer stem cells of human gastrointestinal cancer cells. Black-Right-Pointing-Pointer Nucleotide sequencing revealed that Nanog pseudogene8 but not Nanog was expressed. Black-Right-Pointing-Pointer Nanog pseudogene8 promotes cancer stem cells proliferation. Black-Right-Pointing-Pointer Nanog pseudogene8 is involved in gastrointestinal cancer development.

The globoseries glycosphingolipid SSEA-4 is a marker of bone marrow-derived clonal multipotent stromal cells in vitro and in vivo.

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Rosu-Myles, Michael; McCully, Jennifer; Fair, Joel; Mehic, Jelica; Menendez, Pablo; Rodriguez, Rene; Westwood, Carole

2013-05-01

The therapeutic potential of multipotent stromal cells (MSC) may be enhanced by the identification of markers that allow their discrimination and enumeration both in vivo and in vitro. Here, we investigated the ability of embryonic stem cell-associated glycosphingolipids to isolate human MSC from both whole-bone-marrow (BM) and stromal cell cultures. Only SSEA-4 was consistently expressed on cells within the CD45loCD105hi marrow fraction and could be used to isolate cells with the capacity to give rise to stromal cultures containing MSC. Human stromal cultures, generated in either the presence or absence of serum, contained heterogeneous cell populations discriminated by the quantity of SSEA-4 epitopes detected on their surface. A low level of surface SSEA-4 (SSEA-4lo) correlated with undetectable levels of the α2,3-sialyltransferase-II enzyme required to synthesize SSEA-4; a reduced proliferative potential; and the loss of fat-, bone-, and cartilage-forming cells during long-term culture. In vitro, single cells with the capacity to generate multipotent stromal cultures were detected exclusively in the SSEA-4hi fraction. Our data demonstrate that a high level of surface epitopes for SSEA-4 provides a definitive marker of MSC from human BM.

Analysis list: tra-1 [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available tra-1 Adult,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/tr...a-1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/tra-1.5.tsv http://dbarchive.biosciencedbc....jp/kyushu-u/ce10/target/tra-1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/tra-1.Adult.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/colo/tra-1.Larvae.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/ce10/colo/Adult.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/Larvae.gml ...

Massa óssea em pacientes com anorexia nervosa

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Santos Evaldo dos

2004-01-01

Full Text Available OBJETIVO: avaliar a ingestão diária de cálcio e estabelecer sua correlação com a densidade óssea de pacientes com anorexia nervos. PACIENTES E MÉTODOS: quatorze mulheres com anorexia nervosa registraram, em diário alimentar padronizado, sua ingestão em 24 horas e foram submetidas ao exame de densitometria óssea. A análise estatística foi feita pelos testes do c² e correlação de Pearson, adotando-se como significância estatística p<0,05. RESULTADOS: a média da densidade óssea na coluna lombar foi de 0,95 ± 0,15 e no colo do fêmur foi de 0,88 ± 0,26. Houve correlação significativa entre o tempo de amenorréia e a perda de massa óssea, tanto na coluna lombar (r=-0,65; p=0,01 como no fêmur (r=-0,71; (p=0,0068. Com exceção de uma paciente, todas apresentavam ingestão de cálcio inferior ao recomendado pelo RDA, com média de 554,5 mg/dia (variando de 120 a 840 mg/dia. Observou-se que 64% das pacientes apresentavam algum grau de perda de massa óssea (osteopenia ou osteoporose na coluna e 57% no fêmur. Das seis pacientes com déficit na ingestão de cálcio inferior a 60%, nenhuma apresentou osteoporose, ao passo que das oito pacientes com déficit superior a 60% na ingestão de cálcio, três tinham osteoporose. CONCLUSÃO: Pacientes com anorexia nervosa têm baixa ingestão de cálcio e perda significativa de massa óssea, que estão diretamente relacionadas entre si. A baixa densidade óssea está, também, correlacionada com o tempo de amenorréia.

The osteogenic differentiation of SSEA-4 sub-population of human adipose derived stem cells using silicate nanoplatelets.

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Mihaila, Silvia M; Gaharwar, Akhilesh K; Reis, Rui L; Khademhosseini, Ali; Marques, Alexandra P; Gomes, Manuela E

2014-11-01

How to surpass in vitro stem cell differentiation, reducing cell manipulation, and lead the in situ regeneration process after transplantation, remains to be unraveled in bone tissue engineering (bTE). Recently, we showed that the combination of human bone marrow stromal cells with bioactive silicate nanoplatelets (sNPs) promotes the osteogenic differentiation without the use of standard osteogenic inductors. Even more, using SSEA-4(+) cell-subpopulations (SSEA-4(+)hASCs) residing within the adipose tissue, as a single-cellular source to obtain relevant cell types for bone regeneration, was also proposed. Herein, sNPs were used to promote the osteogenic differentiation of SSEA-4(+)hASCs. The interactions between SSEA-4(+)hASCs and sNPs, namely the internalization pathway and effect on cells osteogenic differentiation, were evaluated. SNPs below 100 μg/mL showed high cytocompatibility and fast internalization via clathrin-mediated pathway. SNPs triggered an overexpression of osteogenic-related markers (RUNX2, osteopontin, osteocalcin) accompanied by increased alkaline phosphatase activity and deposition of a predominantly collagen-type I matrix. Consequently, a robust matrix mineralization was achieved, covering >90% of the culturing surface area. Overall, we demonstrated the high osteogenic differentiation potential of SSEA-4(+)hASCs, further enhanced by the addition of sNPs in a dose dependent manner. This strategy endorses the combination of an adipose-derived cell-subpopulation with inorganic compounds to achieve bone matrix-analogs with clinical relevance. Copyright © 2014 Elsevier Ltd. All rights reserved.

Systematic genetic array analysis links the Saccharomyces cerevisiae SAGA/SLIK and NuA4 component Tra1 to multiple cellular processes

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Andrews Brenda

2008-07-01

Full Text Available Abstract Background Tra1 is an essential 437-kDa component of the Saccharomyces cerevisiae SAGA/SLIK and NuA4 histone acetyltransferase complexes. It is a member of a group of key signaling molecules that share a carboxyl-terminal domain related to phosphatidylinositol-3-kinase but unlike many family members, it lacks kinase activity. To identify genetic interactions for TRA1 and provide insight into its function we have performed a systematic genetic array analysis (SGA on tra1SRR3413, an allele that is defective in transcriptional regulation. Results The SGA analysis revealed 114 synthetic slow growth/lethal (SSL interactions for tra1SRR3413. The interacting genes are involved in a range of cellular processes including gene expression, mitochondrial function, and membrane sorting/protein trafficking. In addition many of the genes have roles in the cellular response to stress. A hierarchal cluster analysis revealed that the pattern of SSL interactions for tra1SRR3413 most closely resembles deletions of a group of regulatory GTPases required for membrane sorting/protein trafficking. Consistent with a role for Tra1 in cellular stress, the tra1SRR3413 strain was sensitive to rapamycin. In addition, calcofluor white sensitivity of the strain was enhanced by the protein kinase inhibitor staurosporine, a phenotype shared with the Ada components of the SAGA/SLIK complex. Through analysis of a GFP-Tra1 fusion we show that Tra1 is principally localized to the nucleus. Conclusion We have demonstrated a genetic association of Tra1 with nuclear, mitochondrial and membrane processes. The identity of the SSL genes also connects Tra1 with cellular stress, a result confirmed by the sensitivity of the tra1SRR3413 strain to a variety of stress conditions. Based upon the nuclear localization of GFP-Tra1 and the finding that deletion of the Ada components of the SAGA complex result in similar phenotypes as tra1SRR3413, we suggest that the effects of tra1SRR3413

Curcumin Effect on the Expressional Profile of OCT4, Nanog and Nucleostemin Genes in AGS (Adenocarcinoma Cancer Cell Line

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Fahmideh Bagrezaei

2016-07-01

Full Text Available Background Curcumin is the natural yellow pigment in turmeric isolated from the rhizome of the plant Curcuma longa. Curcumin inhibits formation and invasive cancer cells and destroys cancer cells resistant to chemotherapeutic drugs. Objectives The purpose of this study was the survey of effects of different concentrations of alcoholic curcumin on the octamer-binding transcription factor 4 (OCT4 Nanog and Nucleostemin genes in the AGS (human gastric adenocarcinoma cell line. Materials and Methods In this experimental study the AGS cell line was cultured in RPMI-1640, supplemented with penicillin/streptomycin (100 U/mL and 100 mg/mL, respectively and 10% fetal bovine serum, at 37°C in a humidified atmosphere of 5% CO2. In 60 - 70% cell confluence, the cells were treated with curcumin concentration (20, 40, 100 μL and incubated for 24, 48 and 72 hours. Finally, total RNA were extracted and cDNA were synthesized and the expression of mentioned genes was detected. The data were analyzed by excel software. Results Expression rate of OCT4A, OCT4B, Nanog and Nucleostemin (GLN3 at concentrations less than 20 μg/mL were reduced but OCT4B1 expression showed increased by hours respectively. Conclusions The results showed that curcumin inhibited cell division; also, this study could be the basis for more extensive studies on the anti-cancer effect of the combined plants.

Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC matrix cells

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Schultz Bruce

2006-02-01

Full Text Available Abstract Background Three transcription factors that are expressed at high levels in embryonic stem cells (ESCs are Nanog, Oct-4 and Sox-2. These transcription factors regulate the expression of other genes during development and are found at high levels in the pluripotent cells of the inner cell mass. The downregulation of these three transcription factors correlates with the loss of pluripotency and self-renewal, and the beginning of subsequent differentiation steps. The roles of Nanog, Oct-4 and Sox-2 have not been fully elucidated. They are important in embryonic development and maintenance of pluripotency in ESCs. We studied the expression of these transcription factors in porcine umbilical cord (PUC matrix cells. Methods Cells were isolated from Wharton's jelly of porcine umbilical cords (PUC and histochemically assayed for the presence of alkaline phosphatase and the presence of Nanog, Oct-4 and Sox-2 mRNA and protein. PCR amplicons were sequenced and compared with known sequences. The synthesis of Oct-4 and Nanog protein was analyzed using immunocytochemistry. FACS analysis was utilized to evaluate Hoechst 33342 dye-stained cells. Results PUC isolates were maintained in culture and formed colonies that express alkaline phosphatase. FACS analysis revealed a side population of Hoechst dye-excluding cells, the Hoechst exclusion was verapamil sensitive. Quantitative and non-quantitative RT-PCR reactions revealed expression of Nanog, Oct-4 and Sox-2 in day 15 embryonic discs, PUC cell isolates and porcine fibroblasts. Immunocytochemical analysis detected Nanog immunoreactivity in PUC cell nuclei, and faint labeling in fibroblasts. Oct-4 immunoreactivity was detected in the nuclei of some PUC cells, but not in fibroblasts. Conclusion Cells isolated from PUC express three transcription factors found in pluripotent stem cell markers both at the mRNA and protein level. The presence of these transcription factors, along with the other

[The characters and specific features of new human embryonic stem cells lines].

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Krylova, T A; Kol'tsova, A M; Zenin, V V; Gordeeva, O F; Musorina, A S; Goriachaia, T S; Shlykova, S A; Kamenetskaia, Iu K; Pinaev, G P; Polianskaia, G G

2009-01-01

Four continuous human embryonic stem cell lines (SC1, SC2, SC3 and SC4), derived from the blastocysts has been described. The cell lines were cultivated on mitotically inactivated human feeder cells. The cell lines SC1 and SC2 have passed through 150 population doublings and the cell lines SC3 and SC4 -- near 120 populations doublings, which exceeds Hayflick limit sufficiently. These cell lines maintain high activity of alkaline phosphatase, expression of transcription factor OCT-4 and cell surface antigens (SSEA-4, TRA-1-60 and TRA-1-81), confirming their ESC status and human specificity. Immunofluorescent detection of antigens, characteristic of ectoderm, endoderm and mesoderm confirms the ability of these cells to retain their pluripotency under in vitro condition. PCR analysis revealed expression of six genes specific for pluripotent cells (OCT-4, NANOG, DPPA3/STELLA, TDGF/CRIPTO and LEFTYA). Correlation between the level of proliferative activity and the character of DNA-bound fluorescent staining was found. Fluorescent dyes, Hoechst 33342 and PI, produced diffuse staining of the nuclei in slowly proliferating cells of the SC1 and SC2 lines. In contrast, in actively proliferating cells of the SC3 and SC4 lines, the clear staining of the nuclei was observed. Upon changing the cultivation condition, proliferative activity of SC3 and SC4 lines decreased and became similar to that of SC1 and SC2 lines. The character of the fluorescent staining of all these lines was also shown to be similar. These results show that quality of the fluorescent staining with Hoechst 33342 and PI reflects the level of proliferation. Possible causes and mechanisms of this feature of human ESC are discussed.

NANOG Is Multiply Phosphorylated and Directly Modified by ERK2 and CDK1 In Vitro

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Justin Brumbaugh

2014-01-01

Full Text Available NANOG is a divergent homeobox protein and a core component of the transcriptional circuitry that sustains pluripotency and self-renewal. Although NANOG has been extensively studied on the transcriptional level, little is known regarding its posttranslational regulation, likely due to its low abundance and challenging physical properties. Here, we identify eleven phosphorylation sites on endogenous human NANOG, nine of which mapped to single amino acids. To screen for the signaling molecules that impart these modifications, we developed the multiplexed assay for kinase specificity (MAKS. MAKS simultaneously tests activity for up to ten kinases while directly identifying the substrate and exact site of phosphorylation. Using MAKS, we discovered site-specific phosphorylation by ERK2 and CDK1/CyclinA2, providing a putative link between key signaling pathways and NANOG.

Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression

NARCIS (Netherlands)

D.L.C. van den Berg (Debbie); W. Zhang (Wensheng); A. Yates (Adam); M.P. Engelen (Erik); K. Takacs (Katalin); K. Bezstarosti (Karel); J.A.A. Demmers (Jeroen); I. Chambers (Ian); R.A. Poor (Raymond)

2008-01-01

textabstractEmbryonic stem (ES) cell self-renewal is regulated by transcription factors, including Oct4, Sox2, and Nanog. A number of additional transcriptional regulators of ES cell self-renewal have recently been identified, including the orphan nuclear receptor estrogen-related receptor beta

Comparison of Oct4, Sox2 and Nanog Expression in Pancreatic Cancer Cell Lines and Human Pancreatic Tumor

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Vahideh Assadollahi

2015-12-01

Full Text Available Background: Genes are involved in the control of stem cell self-renewal as a new class of molecular markers of cancer. Objectives: In this study, the expression of Oct4, Nanog and Sox2 in cell lines MIA Paca-2, PA-TU-8902 and AsPC-1 and pancreatic cancer tissue were examined. Materials and Methods: In this experimental study, cell lines, MIA Paca-2, PA-TU-8902 and AsPC-1, were cultured in DMEM (Dulbecco’s Modified Eagles Medium and RPMI-1640 (Roswell Park Memorial Institute containing FBS 10% (fetal bovine serum in a 37°C incubator containing Co2 5% and humidity 90%. Samples of tumor and non-cancer pancreatic tumor were purchased Iran tumor bank. Extraction of RNA and synthesis of cDNA was performed. Expression levels of Oct4, Nanog and Sox2 were determined using Real-time PCR. The protein expression levels of target genes in the cell lines were studied by flow cytometry and immunocytochemistry. Results: The expression rate of Oct4, Nanog and Sox2 is more in the cancer cell lines than those in the control (normal tissue samples. The protein expression levels of target genes in the cell lines were confirmed by flow cytometry and immunocytochemistry. Conclusions: The genes are involved in stem cell self-renewal as a new class of molecular markers of cancer that detected in the pancreatic cell lines. Maybe, these genes play important role in the uncontrolled proliferation of cancer cells.

Identification of a putative nuclear export signal motif in human NANOG homeobox domain

International Nuclear Information System (INIS)

Park, Sung-Won; Do, Hyun-Jin; Huh, Sun-Hyung; Sung, Boreum; Uhm, Sang-Jun; Song, Hyuk; Kim, Nam-Hyung; Kim, Jae-Hwan

2012-01-01

Highlights: ► We found the putative nuclear export signal motif within human NANOG homeodomain. ► Leucine-rich residues are important for human NANOG homeodomain nuclear export. ► CRM1-specific inhibitor LMB blocked the potent human NANOG NES-mediated nuclear export. -- Abstract: NANOG is a homeobox-containing transcription factor that plays an important role in pluripotent stem cells and tumorigenic cells. To understand how nuclear localization of human NANOG is regulated, the NANOG sequence was examined and a leucine-rich nuclear export signal (NES) motif ( 125 MQELSNILNL 134 ) was found in the homeodomain (HD). To functionally validate the putative NES motif, deletion and site-directed mutants were fused to an EGFP expression vector and transfected into COS-7 cells, and the localization of the proteins was examined. While hNANOG HD exclusively localized to the nucleus, a mutant with both NLSs deleted and only the putative NES motif contained (hNANOG HD-ΔNLSs) was predominantly cytoplasmic, as observed by nucleo/cytoplasmic fractionation and Western blot analysis as well as confocal microscopy. Furthermore, site-directed mutagenesis of the putative NES motif in a partial hNANOG HD only containing either one of the two NLS motifs led to localization in the nucleus, suggesting that the NES motif may play a functional role in nuclear export. Furthermore, CRM1-specific nuclear export inhibitor LMB blocked the hNANOG potent NES-mediated export, suggesting that the leucine-rich motif may function in CRM1-mediated nuclear export of hNANOG. Collectively, a NES motif is present in the hNANOG HD and may be functionally involved in CRM1-mediated nuclear export pathway.

Grafted c-kit+/SSEA1- eye-wall progenitor cells delay retinal degeneration in mice by regulating neural plasticity and forming new graft-to-host synapses.

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Chen, Xi; Chen, Zehua; Li, Zhengya; Zhao, Chen; Zeng, Yuxiao; Zou, Ting; Fu, Caiyun; Liu, Xiaoli; Xu, Haiwei; Yin, Zheng Qin

2016-12-30

Despite diverse pathogenesis, the common pathological change observed in age-related macular degeneration and in most hereditary retinal degeneration (RD) diseases is photoreceptor loss. Photoreceptor replacement by cell transplantation may be a feasible treatment for RD. The major obstacles to clinical translation of stem cell-based cell therapy in RD remain the difficulty of obtaining sufficient quantities of appropriate and safe donor cells and the poor integration of grafted stem cell-derived photoreceptors into the remaining retinal circuitry. Eye-wall c-kit + /stage-specific embryonic antigen 1 (SSEA1) - cells were isolated via fluorescence-activated cell sorting, and their self-renewal and differentiation potential were detected by immunochemistry and flow cytometry in vitro. After labeling with quantum nanocrystal dots and transplantation into the subretinal space of rd1 RD mice, differentiation and synapse formation by daughter cells of the eye-wall c-kit + /SSEA1 - cells were evaluated by immunochemistry and western blotting. Morphological changes of the inner retina of rd1 mice after cell transplantation were demonstrated by immunochemistry. Retinal function of rd1 mice that received cell grafts was tested via flash electroretinograms and the light/dark transition test. Eye-wall c-kit + /SSEA1 - cells were self-renewing and clonogenic, and they retained their proliferative potential through more than 20 passages. Additionally, eye-wall c-kit + /SSEA1 - cells were capable of differentiating into multiple retinal cell types including photoreceptors, bipolar cells, horizontal cells, amacrine cells, Müller cells, and retinal pigment epithelium cells and of transdifferentiating into smooth muscle cells and endothelial cells in vitro. The levels of synaptophysin and postsynaptic density-95 in the retinas of eye-wall c-kit + /SSEA1 - cell-transplanted rd1 mice were significantly increased at 4 weeks post transplantation. The c-kit + /SSEA1 - cells were

Eighteen-Year Cryopreservation Does Not Negatively Affect the Pluripotency of Human Embryos: Evidence from Embryonic Stem Cell Derivation

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Rungsiwiwut, Ruttachuk; Numchaisrika, Pranee; Ahnonkitpanit, Vichuda; Isarasena, Nipan; Virutamasen, Pramuan

2012-01-01

Abstract Human embryonic stem (hES) cells are considered to be a potential source for the therapy of human diseases, drug screening, and the study of developmental biology. In the present study, we successfully derived hES cell lines from blastocysts developed from frozen and fresh embryos. Seventeen- to eighteen-year-old frozen embryos were thawed, cultured to the blastocyst stage, and induced to form hES cells using human foreskin fibroblasts. The Chula2.hES cell line and the Chula4.hES and Chula5.hES cell lines were derived from blastocysts developed from frozen and fresh embryos, respectively. The cell lines expressed pluripotent markers, including alkaline phosphatase (AP), Oct3/4, stage-specific embryonic antigen (SSEA)-4, and tumor recognition antigen (TRA)-1-60 and TRA-1-81 as detected with immunocytochemistry. The real-time polymerase chain reaction (RT-PCR) results showed that the cell lines expressed pluripotent genes, including OCT3/4, SOX2, NANOG, UTF, LIN28, REX1, NODAL, and E-Cadherin. In addition, the telomerase activities of the cell lines were higher than in the fibroblast cells. Moreover, the cell lines differentiated into all three germ layers both in vitro and in vivo. The cell lines had distinct identities, as revealed with DNA fingerprinting, and maintained their normal karyotype after a long-term culture. This study is the first to report the successful derivation of hES cell lines in Thailand and that frozen embryos maintained their pluripotency similar to fresh embryos, as shown by the success of hES cell derivation, even after years of cryopreservation. Therefore, embryos from prolonged cryopreservation could be an alternative source for embryonic stem cell research. PMID:23514952

Hiperplasia de mastócitos na oxalose óssea

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Duarte M.E.L.

1999-01-01

Full Text Available OBJETIVOS: Avaliar através de técnicas de histomorfometria a incidência de hiperplasia de mastócitos na medula óssea de pacientes portadores de oxalose e insuficiência renal crônica. MATERIAL E MÉTODOS: Foram estudados 18 indivíduos divididos em 3 grupos: 6 (4 homens e 2 mulheres com média de idade de 26.31±2.5 anos portadores de oxalose óssea e insuficiência renal crônica (IRC ; 6 (5 mulheres e 1 homem com idade média de 22.1±3.56 anos portadores de IRC e 6 indivíduos saudáveis (5 homens e 1 mulher com idade média de 23±2.78 anos. A análise do tecido ósseo foi realizada em biópsias de crista ilíaca, incluídas em resina, sem descalcificação prévia e coradas pela técnica do Azul de Toluidina. A contagem dos mastócitos foi feita utilizando-se sistema analisador de imagem e os valores (média±DP foram expressos sob a forma de células por mm² de tecido. RESULTADOS: O número de mastócitos foi significativamente maior nos portadores de oxalose óssea, 32.67±9.59, ao se comparar com os pacientes portadores de IRC sem oxalose (20.84±5.04,p<0.05 e nos indivíduos do grupo controle (3.26±1.03,p<0.001 CONCLUSÕES: A oxalose óssea está associada com um aumento substancial do número de mastócitos na medula óssea. Esta alteração não está relacionada com a IRC per se e não parece representar uma resposta inespecífica à fibrose medular. O acúmulo anormal de mastócitos deve, de alguma forma, contribuir para o desenvolvimento da fibrose de medula óssea que acompanha esta condição.

Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.

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Hayashi, Yohei; Caboni, Laura; Das, Debanu; Yumoto, Fumiaki; Clayton, Thomas; Deller, Marc C; Nguyen, Phuong; Farr, Carol L; Chiu, Hsiu-Ju; Miller, Mitchell D; Elsliger, Marc-André; Deacon, Ashley M; Godzik, Adam; Lesley, Scott A; Tomoda, Kiichiro; Conklin, Bruce R; Wilson, Ian A; Yamanaka, Shinya; Fletterick, Robert J

2015-04-14

NANOG (from Irish mythology Tír na nÓg) transcription factor plays a central role in maintaining pluripotency, cooperating with OCT4 (also known as POU5F1 or OCT3/4), SOX2, and other pluripotency factors. Although the physiological roles of the NANOG protein have been extensively explored, biochemical and biophysical properties in relation to its structural analysis are poorly understood. Here we determined the crystal structure of the human NANOG homeodomain (hNANOG HD) bound to an OCT4 promoter DNA, which revealed amino acid residues involved in DNA recognition that are likely to be functionally important. We generated a series of hNANOG HD alanine substitution mutants based on the protein-DNA interaction and evolutionary conservation and determined their biological activities. Some mutant proteins were less stable, resulting in loss or decreased affinity for DNA binding. Overexpression of the orthologous mouse NANOG (mNANOG) mutants failed to maintain self-renewal of mouse embryonic stem cells without leukemia inhibitory factor. These results suggest that these residues are critical for NANOG transcriptional activity. Interestingly, one mutant, hNANOG L122A, conversely enhanced protein stability and DNA-binding affinity. The mNANOG L122A, when overexpressed in mouse embryonic stem cells, maintained their expression of self-renewal markers even when retinoic acid was added to forcibly drive differentiation. When overexpressed in epiblast stem cells or human induced pluripotent stem cells, the L122A mutants enhanced reprogramming into ground-state pluripotency. These findings demonstrate that structural and biophysical information on key transcriptional factors provides insights into the manipulation of stem cell behaviors and a framework for rational protein engineering.

Evolution of the NANOG pseudogene family in the human and chimpanzee genomes

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Maughan Peter J

2006-02-01

Full Text Available Abstract Background The NANOG gene is expressed in mammalian embryonic stem cells where it maintains cellular pluripotency. An unusually large family of pseudogenes arose from it with one unprocessed and ten processed pseudogenes in the human genome. This article compares the NANOG gene and its pseudogenes in the human and chimpanzee genomes and derives an evolutionary history of this pseudogene family. Results The NANOG gene and all pseudogenes except NANOGP8 are present at their expected orthologous chromosomal positions in the chimpanzee genome when compared to the human genome, indicating that their origins predate the human-chimpanzee divergence. Analysis of flanking DNA sequences demonstrates that NANOGP8 is absent from the chimpanzee genome. Conclusion Based on the most parsimonious ordering of inferred source-gene mutations, the deduced evolutionary origins for the NANOG pseudogene family in the human and chimpanzee genomes, in order of most ancient to most recent, are NANOGP6, NANOGP5, NANOGP3, NANOGP10, NANOGP2, NANOGP9, NANOGP7, NANOGP1, and NANOGP4. All of these pseudogenes were fixed in the genome of the human-chimpanzee common ancestor. NANOGP8 is the most recent pseudogene and it originated exclusively in the human lineage after the human-chimpanzee divergence. NANOGP1 is apparently an unprocessed pseudogene. Comparison of its sequence to the functional NANOG gene's reading frame suggests that this apparent pseudogene remained functional after duplication and, therefore, was subject to selection-driven conservation of its reading frame, and that it may retain some functionality or that its loss of function may be evolutionarily recent.

Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells’ Transcription Factors

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Solari, Claudia; Vázquez Echegaray, Camila; Cosentino, María Soledad; Petrone, María Victoria; Waisman, Ariel; Luzzani, Carlos; Francia, Marcos; Villodre, Emilly; Lenz, Guido; Miriuka, Santiago; Barañao, Lino; Guberman, Alejandra

2015-01-01

Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional regulation of the involved genes. The reported modulation of some of these genes led us to hypothesize that some of them could be regulated by the transcription factors critical for self-renewal and pluripotency in embryonic stem cells (ESCs) and in induced pluripotent stem cells (iPSCs). In this work, we studied the expression profile of multiple genes involved in antioxidant defense systems in both ESCs and iPSCs. We found that Manganese superoxide dismutase gene (Mn-Sod/Sod2) was repressed during diverse differentiation protocols showing an expression pattern similar to Nanog gene. Moreover, Sod2 promoter activity was induced by Oct4 and Nanog when we performed a transactivation assay using two different reporter constructions. Finally, we studied Sod2 gene regulation by modulating the expression of Oct4 and Nanog in ESCs by shRNAs and found that downregulation of any of them reduced Sod2 expression. Our results indicate that pluripotency transcription factors positively modulate Sod2 gene transcription. PMID:26642061

Nanog regulates self-renewal of cancer stem cells through the insulin-like growth factor pathway in human hepatocellular carcinoma.

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Shan, Juanjuan; Shen, Junjie; Liu, Limei; Xia, Feng; Xu, Chuan; Duan, Guangjie; Xu, Yanmin; Ma, Qinghua; Yang, Zhi; Zhang, Qianzhen; Ma, Leina; Liu, Jia; Xu, Senlin; Yan, Xiaochu; Bie, Ping; Cui, Youhong; Bian, Xiu-wu; Qian, Cheng

2012-09-01

Hepatocellular carcinoma (HCC) exhibits cellular heterogeneity and embryonic stem-cell-related genes are preferentially overexpressed in a fraction of cancer cells of poorly differentiated tumors. However, it is not known whether or how these cancer cells contribute to tumor initiation and progression. Here, our data showed that increased expression of pluripotency transcription factor Nanog in cancer cells correlates with a worse clinical outcome in HCC. Using the Nanog promoter as a reporter system, we could successfully isolate a small subpopulation of Nanog-positive cells. We demonstrate that Nanog-positive cells exhibited enhanced ability of self-renewal, clonogenicity, and initiation of tumors, which are consistent with crucial hallmarks in the definition of cancer stem cells (CSCs). Nanog(Pos) CSCs could differentiate into mature cancer cells in in vitro and in vivo conditions. In addition, we found that Nanog(Pos) CSCs exhibited resistance to therapeutic agents (e.g., sorafenib and cisplatin) and have a high capacity for tumor invasion and metastasis. Knock-down expression of Nanog in Nanog(Pos) CSCs could decrease self-renewal accompanied with decreased expression of stem-cell-related genes and increased expression of mature hepatocyte-related genes. Overexpression of Nanog in Nanog(Neg) cells could restore self-renewal. Furthermore, we found that insulin-like growth factor (IGF)2 and IGF receptor (IGF1R) were up-regulated in Nanog(Pos) CSCs. Knock-down expression of Nanog in Nanog(Pos) CSCs inhibited the expression of IGF1R, and overexpression of Nanog in Nanog(Neg) cells increased the expression of IGF1R. A specific inhibitor of IGF1R signaling could significantly inhibit self-renewal and Nanog expression, indicating that IGF1R signaling participated in Nanog-mediated self-renewal. These data indicate that Nanog could be a novel biomarker for CSCs in HCC, and that Nanog could play a crucial role in maintaining the self-renewal of CSCs through the IGF1R

The primary role of zebrafish nanog is in extra-embryonic tissue.

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Gagnon, James A; Obbad, Kamal; Schier, Alexander F

2018-01-09

The role of the zebrafish transcription factor Nanog has been controversial. It has been suggested that Nanog is primarily required for the proper formation of the extra-embryonic yolk syncytial layer (YSL) and only indirectly regulates gene expression in embryonic cells. In an alternative scenario, Nanog has been proposed to directly regulate transcription in embryonic cells during zygotic genome activation. To clarify the roles of Nanog, we performed a detailed analysis of zebrafish nanog mutants. Whereas zygotic nanog mutants survive to adulthood, maternal-zygotic (MZ nanog ) and maternal mutants exhibit developmental arrest at the blastula stage. In the absence of Nanog, YSL formation and epiboly are abnormal, embryonic tissue detaches from the yolk, and the expression of dozens of YSL and embryonic genes is reduced. Epiboly defects can be rescued by generating chimeric embryos of MZ nanog embryonic tissue with wild-type vegetal tissue that includes the YSL and yolk cell. Notably, cells lacking Nanog readily respond to Nodal signals and when transplanted into wild-type hosts proliferate and contribute to embryonic tissues and adult organs from all germ layers. These results indicate that zebrafish Nanog is necessary for proper YSL development but is not directly required for embryonic cell differentiation. © 2018. Published by The Company of Biologists Ltd.

Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos

International Nuclear Information System (INIS)

Fang, Zhen F.; Gai, Hui; Huang, You Z.; Li, Shan G.; Chen, Xue J.; Shi, Jian J.; Wu, Li; Liu, Ailian; Xu, Ping; Sheng, Hui Z.

2006-01-01

Embryonic stem cells were isolated from rabbit blastocysts derived from fertilization (conventional rbES cells), parthenogenesis (pES cells) and nuclear transfer (ntES cells), and propagated in a serum-free culture system. Rabbit ES (rbES) cells proliferated for a prolonged time in an undifferentiated state and maintained a normal karyotype. These cells grew in a monolayer with a high nuclear/cytoplasm ratio and contained a high level of alkaline phosphate activity. In addition, rbES cells expressed the pluripotent marker Oct-4, as well as EBAF2, FGF4, TDGF1, but not antigens recognized by antibodies against SSEA-1, SSEA-3, SSEA-4, TRA-1-10 and TRA-1-81. All 3 types of ES cells formed embryoid bodies and generated teratoma that contained tissue types of all three germ layers. rbES cells exhibited a high cloning efficiency, were genetically modified readily and were used as nuclear donors to generate a viable rabbit through somatic cell nuclear transfer. In combination with genetic engineering, the ES cell technology should facilitate the creation of new rabbit lines

ROCK inhibitor Y-27632 enhances the survivability of dissociated buffalo (Bubalus bubalis) embryonic stem cell-like cells.

Science.gov (United States)

Sharma, Ruchi; George, Aman; Chauhan, Manmohan S; Singla, Suresh; Manik, Radhey S; Palta, Prabhat

2013-01-01

This study investigated the effects of supplementation of culture medium with 10 μM Y-27632, a specific inhibitor of Rho kinase activity, for 6 days on self-renewal of buffalo embryonic stem (ES) cell-like cells at Passage 50-80. Y-27632 increased mean colony area (P<0.05) although it did not improve their survival. It decreased OCT4 expression (P<0.05), increased NANOG expression (P<0.05), but had no effect on SOX2 expression. It also increased expression of anti-apoptotic gene BCL-2 (P<0.05) and decreased that of pro-apoptotic genes BAX and BID (P<0.05). It increased plating efficiency of single-cell suspensions of ES cells (P<0.05). Following vitrification, the presence of Y-27632 in the vitrification solution or thawing medium or both did not improve ES cell colony survival. However, following seeding of clumps of ES cells transfected with pAcGFP1N1 carrying green fluorescent protein (GFP), Y-27632 increased colony formation rate (P<0.01). ES cell colonies that formed in all Y-27632-supplemented groups were confirmed for expression of pluripotency markers alkaline phosphatase, SSEA-4 and TRA-1-60, and for their ability to generate embryoid bodies containing cells that expressed markers of ectoderm, mesoderm and endoderm. In conclusion, Y-27632 improves survival of buffalo ES cells under unfavourable conditions such as enzymatic dissociation to single cells or antibiotic-assisted selection after transfection, without compromising their pluripotency.

Three-dimensional localisation of NANOG, OCT4, and E-CADHERIN in porcine pre- and peri-implantation embryos

DEFF Research Database (Denmark)

Wolf, Xenia Asbæk; Serup, Palle; Hyttel, Poul

2011-01-01

. The expression of NANOG differed remarkably from that reported in other species. NANOG was not detected in the inner cell mass of hatched porcine blastocysts, but later appeared in the epiblast and hypoblast of spherical blastocysts where Rauber's layer had disintegrated. In pre-gastrulating, filamentous embryos...

Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours

DEFF Research Database (Denmark)

Hoei-Hansen, C E; Almstrup, K; Nielsen, J E

2005-01-01

AIMS: NANOG is a key regulator of embryonic stem cell (ESC) self-renewal and pluripotency. Our recent genome-wide gene expression profiling study of the precursor of testicular germ cell tumours, carcinoma in situ testis (CIS), showed close similarity between ESC and CIS, including high NANOG...... earlier than for OCT-4. We detected no expression at the protein level in normal testis. CONCLUSIONS: NANOG is a new marker for testicular CIS and germ cell tumours and the high level of NANOG along with OCT-4 are determinants of the stem cell-like pluripotency of the preinvasive CIS cell. Timing of NANOG...... expression. In the present study we analysed the protein expression of NANOG during normal development of human testis and in a large series of neoplastic/dysgenetic specimens. METHODS AND RESULTS: We detected abundant expression of NANOG in CIS and in CIS-derived testicular tumours with marked differences...

Knockdown of p53 suppresses Nanog expression in embryonic stem cells

Energy Technology Data Exchange (ETDEWEB)

Abdelalim, Essam Mohamed, E-mail: emohamed@qf.org.qa [Qatar Biomedical Research Institute, Qatar Foundation, Doha 5825 (Qatar); Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan); Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia (Egypt); Tooyama, Ikuo [Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan)

2014-01-10

Highlights: •We investigate the role of p53 in ESCs in the absence of DNA damage. •p53 knockdown suppresses ESC proliferation. •p53 knockdown downregulates Nanog expression. •p53 is essential for mouse ESC self-renewal. -- Abstract: Mouse embryonic stem cells (ESCs) express high levels of cytoplasmic p53. Exposure of mouse ESCs to DNA damage leads to activation of p53, inducing Nanog suppression. In contrast to earlier studies, we recently reported that chemical inhibition of p53 suppresses ESC proliferation. Here, we confirm that p53 signaling is involved in the maintenance of mouse ESC self-renewal. RNA interference-mediated knockdown of p53 induced downregulation of p21 and defects in ESC proliferation. Furthermore, p53 knockdown resulted in a significant downregulation in Nanog expression at 24 and 48 h post-transfection. p53 knockdown also caused a reduction in Oct4 expression at 48 h post-transfection. Conversely, exposure of ESCs to DNA damage caused a higher reduction of Nanog expression in control siRNA-treated cells than in p53 siRNA-treated cells. These data show that in the absence of DNA damage, p53 is required for the maintenance of mouse ESC self-renewal by regulating Nanog expression.

Establishment of human induced pluripotent stem cell lines from normal fibroblast TIG-1.

Science.gov (United States)

Kumazaki, Tsutomu; Kurata, Sayaka; Matsuo, Taira; Mitsui, Youji; Takahashi, Tomoko

2011-06-01

Normal human cells have a replicative life span and therefore senesce. Usually, normal human cell strains are differentiated cells and reach a terminally differentiated state after a number of cell divisions. At present, definitive differences are not known between replicative senescence and terminal differentiation. TIG-1 is a human fibroblast strain established from fetal lung and has been used extensively in studies of cellular senescence, and numerous data were accumulated at the molecular level. Recently, a method for generating induced pluripotent stem cells (iPSCs) was developed. Using the method, we introduced four reprogramming genes to TIG-1 fibroblasts and succeeded in isolating colonies that had embryonic stem cell (ESC)-like morphologies. They showed alkaline phosphatase activity and expressed ESC markers, as shown by immunostaining of OCT4, SOX2, SSEA4, and TRA-1-81 as well as reverse-transcription polymerase chain reaction (RT-PCR) for OCT4 and NANOG transcripts. Thus, we succeeded in establishing iPSC clones from TIG-1. The iPSC clones could differentiate to cells originated from all three germ-cell layers, as shown by RT-PCR, for messenger RNA (mRNA) expression of α-fetoprotein (endoderm), MSX1 (mesoderm) and microtubule-associated protein 2 (ectoderm), and by immunostaining for α-fetoprotein (endoderm), α-smooth muscle actin (mesoderm), and β-III-tubulin (ectoderm). The iPSCs formed teratoma containing the structures developed from all three germ-cell layers in severe combined immune-deficiency mice. Thus, by comparing the aging process of parental TIG-1 cells and the differentiation process of iPSC-derived fibrocytes to fibroblasts, we can reveal the exact differences in processes between senescence and terminal differentiation.

Development of the SyncTraX FX4 version real-time tumor tracking system for radiation therapy

International Nuclear Information System (INIS)

Ishiyama, Tomoharu; Torigoe, Yui; Nagae, Koudai; Kajiki, Shunsuke; Sano, Takayuki

2017-01-01

Based on the current SyncTraX that was developed jointly with Hokkaido University, we have developed the SyncTraX FX4 version, which features not only tumor tracking but also patient positioning. The SyncTraX FX4 version is configured with four fixed x-ray tubes an detectors and eliminated the rail that is used to move the x-ray tube and detector as in the current SyncTraX. This development simplifies limitations on facility construction. We also adopted a distortion free flat panel detector instead of a color I.I., and the additional patient positioning feature will enable the SyncTraX FX4 version to become more widely applied in clinical cases. (author)

Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC

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Khodadad Khodadadi

2012-01-01

Full Text Available Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts via retroviral transduction with three transcription factors using OCT4, SOX2, and KLF4 in the absence of c-MYC. The cell lines were maintained beyond 27 passages (more than 11 months and characterized. The equine iPS (EiPS cells stained positive for alkaline phosphatase by histochemical staining and expressed OCT4, NANOG, SSEA1, and SSEA4. Gene expression analysis of the cells showed the expression of OCT4, SOX2 NANOG, and STAT3. The cell lines retained a euploid chromosome count of 64 after long-term culture cryopreservation. The EiPS demonstrated differentiation capacity for the three embryonic germ layers both in vitro by embryoid bodies (EBs formation and in vivo by teratoma formation. In conclusion, we report the derivation of iPS cells from equine adult fibroblasts and long-term maintenance using either of the three reprogramming factors.

Reduction of NANOG Mediates the Inhibitory Effect of Aspirin on Tumor Growth and Stemness in Colorectal Cancer

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Hefei Wang

2017-11-01

Full Text Available Background/Aims: Cancer stem cells (CSCs are considered to be responsible for tumor relapse and metastasis, which serve as a potential therapeutic target for cancer. Aspirin has been shown to reduce cancer risk and mortality, particularly in colorectal cancer. However, the CSCs-suppressing effect of aspirin and its relevant mechanisms in colorectal cancer remain unclear. Methods: CCK8 assay was employed to detect the cell viability. Sphere formation assay, colony formation assay, and ALDH1 assay were performed to identify the effects of aspirin on CSC properties. Western blotting was performed to detect the expression of the stemness factors. Xenograft model was employed to identify the anti-cancer effects of aspirin in vivo. Unpaired Student t test, ANOVA test and Kruskal-Wallis test were used for the statistical comparisons. Results: Aspirin attenuated colonosphere formation and decreased the ALDH1 positive cell population of colorectal cancer cells. Aspirin inhibited xenograft tumor growth and reduced tumor cells stemness in nude mice. Consistently, aspirin decreased the protein expression of stemness-related transcription factors, including c-Myc, OCT4 and NANOG. Suppression of NANOG blocked the effect of aspirin on sphere formation. Conversely, ectopic expression of NANOG rescued the aspirin-repressed sphere formation, suggesting that NANOG is a key downstream target. Moreover, we found that aspirin repressed NANOG expression in protein level by decreasing its stability. Conclusion: We have provided new evidence that aspirin attenuates CSC properties through down-regulation of NANOG, suggesting aspirin as a promising therapeutic agent for colorectal cancer treatment.

File list: Oth.PSC.20.Nanog.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Oth.PSC.20.Nanog.AllCell mm9 TFs and others Nanog Pluripotent stem cell SRX213808,S...1351,SRX1141349,SRX1141350,SRX651982,SRX213820 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.Nanog.AllCell.bed ...

File list: Oth.PSC.05.Nanog.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Oth.PSC.05.Nanog.AllCell mm9 TFs and others Nanog Pluripotent stem cell SRX248286,S...3864,SRX1141351,SRX1141350,SRX651982,SRX213820 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.Nanog.AllCell.bed ...

Expression of Nanog gene promotes NIH3T3 cell proliferation

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Zhang Jingyu; Wang Xia; Chen Bing; Suo Guangli; Zhao Yanhong; Duan Ziyuan; Dai Jianwu

2005-01-01

Cells are the functional elements in tissue engineering and regenerative medicine. A large number of cells are usually needed for these purposes. However, there are numbers of limitations for in vitro cell proliferation. Nanog is an important self-renewal determinant in embryonic stem cells. However, it remains unknown whether Nanog will influence the cell cycle and cell proliferation of mature cells. In this study, we expressed Nanog in NIH3T3 cells and showed that expression of Nanog in NIH3T3 promoted cells to enter into S phase and enhanced cell proliferation. This suggests that Nanog gene might function in a similar fashion in mature cells as in ES cells. In addition, it may provide an approach for in vitro cell expansion

File list: Oth.ALL.05.Nanog.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Oth.ALL.05.Nanog.AllCell mm9 TFs and others Nanog All cell types SRX248286,SRX24828...X1141351,SRX1141350,SRX651982,SRX1008191,SRX213820,SRX1008192 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.05.Nanog.AllCell.bed ...

File list: Oth.ALL.10.Nanog.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Oth.ALL.10.Nanog.AllCell mm9 TFs and others Nanog All cell types SRX248286,SRX21380...X1141350,SRX1141351,SRX651982,SRX1008192,SRX213820,SRX1008191 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.10.Nanog.AllCell.bed ...

File list: Oth.ALL.20.Nanog.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Oth.ALL.20.Nanog.AllCell mm9 TFs and others Nanog All cell types SRX213808,SRX21382...X1141349,SRX1141350,SRX651982,SRX213820,SRX1008191,SRX1008192 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.20.Nanog.AllCell.bed ...

NANOG priming before full reprogramming may generate germ cell tumours

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I Grad

2011-11-01

Full Text Available Reprogramming somatic cells into a pluripotent state brings patient-tailored, ethical controversy-free cellular therapy closer to reality. However, stem cells and cancer cells share many common characteristics; therefore, it is crucial to be able to discriminate between them. We generated two induced pluripotent stem cell (iPSC lines, with NANOG pre-transduction followed by OCT3/4, SOX2, and LIN28 overexpression. One of the cell lines, CHiPS W, showed normal pluripotent stem cell characteristics, while the other, CHiPS A, though expressing pluripotency markers, failed to differentiate and gave rise to germ cell-like tumours in vivo. Comparative genomic hybridisation analysis of the generated iPS lines revealed that they were genetically more stable than human embryonic stem cell counterparts. This analysis proved to be predictive for the differentiation potential of analysed cells. Moreover, the CHiPS A line expressed a lower ratio of p53/p21 when compared to CHiPS W. NANOG pre-induction followed by OCT3/4, SOX2, MYC, and KLF4 induction resulted in the same tumour-inducing phenotype. These results underline the importance of a re-examination of the role of NANOG during reprogramming. Moreover, this reprogramming method may provide insights into primordial cell tumour formation and cancer stem cell transformation.

Estudo da densidade óssea na esclerodermia sistêmica

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da Silva H.C.

1997-01-01

Full Text Available OBJETIVO. A osteopenia em pacientes com esclerodermia sistêmica foi descrita, radiologicamente, em mãos e, por densidade óssea, no terço proximal e distal do rádio. A redução da massa óssea, nesses pacientes, tem sido atribuída à isquemia, imobilização e à menopausa precoce. O objetivo deste estudo é analisar a densidade óssea na coluna, região proximal do fêmur e corpo todo de pacientes com esclerodermia sistêmica. PACIENTES E MÉTODO. Foram examinadas 25 pacientes caucasóides, sem outras condições que pudessem afetar o metabolismo ósseo. A média de idade das pacientes foi de 48 ± 12 anos, e o tempo de doença, de 7 ± 7 anos; 13 estavam na pós-menopausa há 8 ± 8 anos. A medida de massa óssea foi realizada na coluna, região proximal do fêmur e corpo todo, utilizando-se densitômetro de dupla emissão com fonte de raios X (Lunar - modelo DPX. RESULTADOS. Não houve diferença estatisticamente significante na densidade óssea das regiões avaliadas nas pacientes com esclerodermia sistêmica e as mulheres-controle pareadas para a idade, peso, altura e anos de menopausa. A densidade óssea das pacientes com forma limitada não foi diferente daquelas com a forma difusa. Pacientes com calcinose apresentaram menor densidade óssea na região proximal do fêmur que aquelas sem calcinose. CONCLUSÕES. Os autores concluíram que pacientes com esclerodermia sistêmica não apresentam perda de massa óssea. Portanto, a esclerodermia não é um fator de risco para o desenvolvimento de osteoporose generalizada.

Nanog interact with CDK6 to regulates astrocyte cells proliferation following spinal cord injury

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Gu, Jun [Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu (China); Department of Orthopaedics, Xishan People' s Hospital, Wuxi, Jiangsu (China); Ni, Yingjie; Xu, Lin; Xu, Hongliang [Department of Orthopaedics, Xishan People' s Hospital, Wuxi, Jiangsu (China); Cai, Zhengdong, E-mail: caizhengdongsh@163.com [Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu (China)

2016-01-22

Previous research had reported transcription factors Nanog expressed in pluripotent embryonic stem cells (ESCS) that played an important role in regulating the cell proliferation. Nanog levels are frequently elevated in ESCS, but the role in the spinal cord was not clear. To examine the biological relevance of Nanog, we studied its properties in spinal cord injury model. The expression of Nanog and PCNA was gradually increased and reached a peak at 3 day by western blot analysis. The expression of Nanog was further analyzed by immunohistochemistry. Double immunofluorescent staining uncovered that Nanog can co-labeled with PCNA and GFAP in the spinal cord tissue. In vitro, Nanog can promote the proliferation of astrocyte cell by Fluorescence Activating Cell Sorter (FACS) and CCK8. Meanwhile, the cell-cycle protein CDK6 could interact with Nanog in the spinal cord tissue. Taken together, these data suggested that both Nanog may play important roles in spinal cord pathophysiology via interact with CDK6.

Nanog interact with CDK6 to regulates astrocyte cells proliferation following spinal cord injury

International Nuclear Information System (INIS)

Gu, Jun; Ni, Yingjie; Xu, Lin; Xu, Hongliang; Cai, Zhengdong

2016-01-01

Previous research had reported transcription factors Nanog expressed in pluripotent embryonic stem cells (ESCS) that played an important role in regulating the cell proliferation. Nanog levels are frequently elevated in ESCS, but the role in the spinal cord was not clear. To examine the biological relevance of Nanog, we studied its properties in spinal cord injury model. The expression of Nanog and PCNA was gradually increased and reached a peak at 3 day by western blot analysis. The expression of Nanog was further analyzed by immunohistochemistry. Double immunofluorescent staining uncovered that Nanog can co-labeled with PCNA and GFAP in the spinal cord tissue. In vitro, Nanog can promote the proliferation of astrocyte cell by Fluorescence Activating Cell Sorter (FACS) and CCK8. Meanwhile, the cell-cycle protein CDK6 could interact with Nanog in the spinal cord tissue. Taken together, these data suggested that both Nanog may play important roles in spinal cord pathophysiology via interact with CDK6.

Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG

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Wen-Ying Liao

2013-01-01

Full Text Available Breast cancer stem cells (CSCs are highly tumorigenic and possess the capacity to self-renew. Recent studies indicated that pluripotent gene NANOG involves in regulating self-renewal of breast CSCs, and expression of NANOG is correlated with aggressiveness of poorly differentiated breast cancer. We initially confirmed that breast cancer MCF-7 cells expressed NANOG, and overexpression of NANOG enhanced the tumorigenicity of MCF-7 cells and promoted the self-renewal expansion of CD24−/lowCD44+ CSC subpopulation. In contrast, knockdown of NANOG significantly affected the growth of breast CSCs. Utilizing flow cytometry, we identified five cyclohexylmethyl flavonoids that can inhibit propagation of NANOG-positive cells in both breast cancer MCF-7 and MDA-MB231 cells. Among these flavonoids, ugonins J and K were found to be able to induce apoptosis in non-CSC populations and to reduce self-renewal growth of CD24−/lowCD44+ CSC population. Treatment with ugonin J significantly reduced the tumorigenicity of MCF-7 cells and efficiently suppressed formation of mammospheres. This suppression was possibly due to p53 activation and NANOG reduction as either addition of p53 inhibitor or overexpression of NANOG can counteract the suppressive effect of ugonin J. We therefore conclude that cyclohexylmethyl flavonoids can possibly be utilized to suppress the propagation of breast CSCs via reduction of NANOG.

Neoformação óssea e osteointegração de biomateriais micro e nanoestruturados em ovinos

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Bruna D. Costa

2015-02-01

Full Text Available A substituição de parte do tecido ósseo se faz necessária muitas vezes na rotina cirúrgica, seja em função de fraturas, neoplasias ósseas ou enfermidades ortopédicas que acarretem em perda óssea. Neste sentido, têm se buscado biomateriais capazes de promover esta substituição, evitando o uso de enxertos ou transplantes ósseos. O objetivo deste trabalho foi avaliar a capacidade osteoregenerativa de biomateriais em diferentes composições, em tíbias de ovinos. Foram utilizadas oito ovelhas mestiças texel, com 12 meses de idade e peso médio de 28,5±7,4kg. Após adequada preparação anestésica e cirúrgica, foram produzidos três defeitos ósseos na diáfise das tíbias em sua face medial, totalizando seis defeitos de 6mm cada, sendo que quatro foram preenchidos por biomateriais, e dois por fragmentos ósseos retirados do próprio animal (autocontrole. Os materiais implantados foram: hidroxiapatita (HA, tricálcio fosfato-β (TCP-β, hidroxiapatita/tricálcio fosfato-b 60/40 (HA/TCP-b 60/40 e o nanocompósito hidroxiapatita/alumina a 5% (HA/Al2O3 a 5%. Os animais foram alocados em dois grupos: Grupo 60 (n=04, em que os animais foram eutanasiados após 60 dias da colocação dos implantes e Grupo 90 (n=04, em que a eutanásia ocorreu 90 dias após a colocação dos implantes. Foram realizadas radiografias nos períodos pré-operatório, imediatamente após o procedimento e aos 30, 60 e 90 dias de pós-operatório, a fim de excluir qualquer alteração prévia ou complicação pós-operatória, capaz de comprometer o estudo. Após a eutanásia, foram coletadas as tíbias para avaliação macro e microscópica, por meio de microscopia eletrônica de varredura (MEV e microscopia óptica. Os resultados encontrados mostraram uma boa capacidade de neoformação óssea e uma lenta absorção da HA. O TCP-β foi rapidamente absorvido e apresentou boa capacidade osteoindutiva e osteocondutiva, sendo observada neoformação óssea no

Hyaluronan-CD44v3 Interaction with Oct4-Sox2-Nanog Promotes miR-302 Expression Leading to Self-renewal, Clonal Formation, and Cisplatin Resistance in Cancer Stem Cells from Head and Neck Squamous Cell Carcinoma*

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Bourguignon, Lilly Y. W.; Wong, Gabriel; Earle, Christine; Chen, Liqun

2012-01-01

Human head and neck squamous cell carcinoma (HNSCC) is a highly malignant cancer associated with major morbidity and mortality. In this study, we determined that human HNSCC-derived HSC-3 cells contain a subpopulation of cancer stem cells (CSCs) characterized by high levels of CD44v3 and aldehyde dehydrogenase-1 (ALDH1) expression. These tumor cells also express several stem cell markers (the transcription factors Oct4, Sox2, and Nanog) and display the hallmark CSC properties of self-renewal/clonal formation and the ability to generate heterogeneous cell populations. Importantly, hyaluronan (HA) stimulates the CD44v3 (an HA receptor) interaction with Oct4-Sox2-Nanog leading to both a complex formation and the nuclear translocation of three CSC transcription factors. Further analysis reveals that microRNA-302 (miR-302) is controlled by an upstream promoter containing Oct4-Sox2-Nanog-binding sites, whereas chromatin immunoprecipitation (ChIP) assays demonstrate that stimulation of miR-302 expression by HA-CD44 is Oct4-Sox2-Nanog-dependent in HNSCC-specific CSCs. This process results in suppression of several epigenetic regulators (AOF1/AOF2 and DNMT1) and the up-regulation of several survival proteins (cIAP-1, cIAP-2, and XIAP) leading to self-renewal, clonal formation, and cisplatin resistance. These CSCs were transfected with a specific anti-miR-302 inhibitor to silence miR-302 expression and block its target functions. Our results demonstrate that the anti-miR-302 inhibitor not only enhances the expression of AOF1/AOF2 and DNMT1 but also abrogates the production of cIAP-1, cIAP-2, and XIAP and HA-CD44v3-mediated cancer stem cell functions. Taken together, these findings strongly support the contention that the HA-induced CD44v3 interaction with Oct4-Sox2-Nanog signaling plays a pivotal role in miR-302 production leading to AOF1/AOF2/DNMT1 down-regulation and survival of protein activation. All of these events are critically important for the acquisition of cancer

Hyaluronan-CD44v3 interaction with Oct4-Sox2-Nanog promotes miR-302 expression leading to self-renewal, clonal formation, and cisplatin resistance in cancer stem cells from head and neck squamous cell carcinoma.

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Bourguignon, Lilly Y W; Wong, Gabriel; Earle, Christine; Chen, Liqun

2012-09-21

Human head and neck squamous cell carcinoma (HNSCC) is a highly malignant cancer associated with major morbidity and mortality. In this study, we determined that human HNSCC-derived HSC-3 cells contain a subpopulation of cancer stem cells (CSCs) characterized by high levels of CD44v3 and aldehyde dehydrogenase-1 (ALDH1) expression. These tumor cells also express several stem cell markers (the transcription factors Oct4, Sox2, and Nanog) and display the hallmark CSC properties of self-renewal/clonal formation and the ability to generate heterogeneous cell populations. Importantly, hyaluronan (HA) stimulates the CD44v3 (an HA receptor) interaction with Oct4-Sox2-Nanog leading to both a complex formation and the nuclear translocation of three CSC transcription factors. Further analysis reveals that microRNA-302 (miR-302) is controlled by an upstream promoter containing Oct4-Sox2-Nanog-binding sites, whereas chromatin immunoprecipitation (ChIP) assays demonstrate that stimulation of miR-302 expression by HA-CD44 is Oct4-Sox2-Nanog-dependent in HNSCC-specific CSCs. This process results in suppression of several epigenetic regulators (AOF1/AOF2 and DNMT1) and the up-regulation of several survival proteins (cIAP-1, cIAP-2, and XIAP) leading to self-renewal, clonal formation, and cisplatin resistance. These CSCs were transfected with a specific anti-miR-302 inhibitor to silence miR-302 expression and block its target functions. Our results demonstrate that the anti-miR-302 inhibitor not only enhances the expression of AOF1/AOF2 and DNMT1 but also abrogates the production of cIAP-1, cIAP-2, and XIAP and HA-CD44v3-mediated cancer stem cell functions. Taken together, these findings strongly support the contention that the HA-induced CD44v3 interaction with Oct4-Sox2-Nanog signaling plays a pivotal role in miR-302 production leading to AOF1/AOF2/DNMT1 down-regulation and survival of protein activation. All of these events are critically important for the acquisition of cancer

Fadiga óssea: causa de dor em joelhos na osteoartrite

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Wilson Campos Tavares Júnior

2012-10-01

Full Text Available A dor no joelho é o sintoma mais comum na osteoartrite, sendo a principal causa de incapacidade crônica em idosos e uma das principais fontes de morbidade atribuível à osteoartrite em geral. As causas de dor no joelho em pessoas com osteoartrite não são facilmente entendidas e o conhecimento sobre as causas da dor é fundamental para que futuramente sejam realizadas intervenções específicas. A fadiga óssea representa o remodelamento do osso subcondral na osteoartrite, levando a uma consequente alteração na forma do osso e/ou perda óssea. No entanto, a fadiga óssea não é algo facilmente interpretado, pois é de difícil detecção na ausência de defeitos claros da cortical e pela sobreposição de estruturas ósseas nas radiografias convencionais. A fadiga óssea está associada não apenas a dor no joelho, mas também a rigidez e incapacidade. Se a fadiga ocorre antes da osteoartrite avançada, isso sugere que alterações no osso subcondral podem ocorrer simultaneamente a alterações da cartilagem e que tratamentos visando sua preservação podem não ser eficazes. Lesões com padrão de edema ósseo estão associadas e são fatores preditivos para fadiga óssea. Este trabalho tem por objetivo rever a literatura mostrando a importância da fadiga óssea e de como diagnosticar esta alteração nos exames de imagem.

The pluripotency factor Nanog is directly upregulated by the androgen receptor in prostate cancer cells.

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Kregel, Steven; Szmulewitz, Russell Z; Vander Griend, Donald J

2014-11-01

The Androgen Receptor (AR) is a nuclear hormone receptor that functions as a critical oncogene in all stages of prostate cancer progression, including progression to castration-resistance following androgen-deprivation therapy. Thus, identifying and targeting critical AR-regulated genes is one potential method to block castration-resistant cancer proliferation. Of particular importance are transcription factors that regulate stem cell pluripotency; many of these genes are emerging as critical oncogenes in numerous tumor cell types. Of these, Nanog has been previously shown to increase the self-renewal and stem-like properties of prostate cancer cells. Thus, we hypothesized that Nanog is a candidate AR target gene that may impart castration-resistance. We modulated AR signaling in LNCaP prostate cancer cells and assayed for Nanog expression. Direct AR binding to the NANOG promoter was tested using AR Chromatin Immunoprecipation (ChIP) and analyses of publically available AR ChIP-sequencing data-sets. Nanog over-expressing cells were analyzed for cell growth and cytotoxicity in response to the AR antagonist enzalutamide and the microtubule stabilizing agent docetaxel. AR signaling upregulates Nanog mRNA and protein. AR binds directly to the NANOG promoter, and was not identified within 75 kb of the NANOGP8 pseudogene, suggesting the NANOG gene locus was preferentially activated. Nanog overexpression in LNCaP cells increases overall growth, but does not increase resistance to enzalutamide or docetaxel. Nanog is a novel oncogenic AR target gene in prostate cancer cells, and stable expression of Nanog increases proliferation and growth of prostate cancer cells, but not resistance to enzalutamide or docetaxel. © 2014 Wiley Periodicals, Inc.

Crystal structure and DNA binding of the homeodomain of the stem cell transcription factor Nanog.

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Jauch, Ralf; Ng, Calista Keow Leng; Saikatendu, Kumar Singh; Stevens, Raymond C; Kolatkar, Prasanna R

2008-02-22

The transcription factor Nanog is an upstream regulator in early mammalian development and a key determinant of pluripotency in embryonic stem cells. Nanog binds to promoter elements of hundreds of target genes and regulates their expression by an as yet unknown mechanism. Here, we report the crystal structure of the murine Nanog homeodomain (HD) and analysis of its interaction with a DNA element derived from the Tcf3 promoter. Two Nanog amino acid pairs, unique among HD sequences, appear to affect the mechanism of nonspecific DNA recognition as well as maintain the integrity of the structural scaffold. To assess selective DNA recognition by Nanog, we performed electrophoretic mobility shift assays using a panel of modified DNA binding sites and found that Nanog HD preferentially binds the TAAT(G/T)(G/T) motif. A series of rational mutagenesis experiments probing the role of six variant residues of Nanog on its DNA binding function establish their role in affecting binding affinity but not binding specificity. Together, the structural and functional evidence establish Nanog as a distant member of a Q50-type HD despite having considerable variation at the sequence level.

Crystal Structure and DNA Binding of the Homeodomain of the Stem Cell Transcription Factor Nanog

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Jauch, Ralf; Ng, Calista Keow Leng; Saikatendu, Kumar Singh; Stevens, Raymond C.; Kolatkar, Prasanna R. (GI-Singapore); (Scripps)

2010-02-08

The transcription factor Nanog is an upstream regulator in early mammalian development and a key determinant of pluripotency in embryonic stem cells. Nanog binds to promoter elements of hundreds of target genes and regulates their expression by an as yet unknown mechanism. Here, we report the crystal structure of the murine Nanog homeodomain (HD) and analysis of its interaction with a DNA element derived from the Tcf3 promoter. Two Nanog amino acid pairs, unique among HD sequences, appear to affect the mechanism of nonspecific DNA recognition as well as maintain the integrity of the structural scaffold. To assess selective DNA recognition by Nanog, we performed electrophoretic mobility shift assays using a panel of modified DNA binding sites and found that Nanog HD preferentially binds the TAAT(G/T)(G/T) motif. A series of rational mutagenesis experiments probing the role of six variant residues of Nanog on its DNA binding function establish their role in affecting binding affinity but not binding specificity. Together, the structural and functional evidence establish Nanog as a distant member of a Q50-type HD despite having considerable variation at the sequence level.

Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient

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Zhong Liu

2016-09-01

Full Text Available Human iPSC line TSD-01-hiPSC was generated from fibroblasts of a patient with infantile Tay-Sachs disease (TSD. The patient is compound heterozygous at the HEXA gene by carrying a 1278insTATC allele and an IVS12+1G>C allele. STEMCCA lentivirus, which expresses OCT4, SOX2, KLF4, and c-MYC from a polycistronic transcript, were used for reprogramming. TSD-01-hiPSC express pluripotency markers such as OCT4, SOX2, NANOG, Tra-1-60, and alkaline phosphatase, and can differentiate into tissues from all the three embryonic germ layers. This TSD patient-derived hiPSC line may serve as a valuable in vitro tool for disease modeling and drug test.

[The Effect of TALENs-mediated Downregulation Expression of Nanog on Malignant Behavior of Cervical Cancer HeLa Cells].

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Yu, Ai-qing; Li, Cheng-lin; Yang, Yi; Yan, Shi-rong

2016-01-01

To study the effect of downregulation expression of Nanog on malignant behavior of cervical cancer HeLa cells. Gene editing tool TALENs was employed to induce downregulation expression of Nanog, and Nanog mutation was evaluated by sequencing. RT-PCR and Western blot was used to detect the mRNA and protein expression level, respectively. Colony-formation assay, Transwell invasion assay, and chemotherapy sensibility assay was carried out to assess the capacity of colony-formation, invasion, and chemoresistance, respectively. TALENs successfully induced Nanog mutation and downregulated Nanog expression. Nanog mRNA and protein expression of Nanog-mutated monoclonal HeLa cells downregulated 3 times compared to thoses of wild-type HeLa cells (P HeLa cells were observed when compared to those of wild-type HeLa cells (P HeLa cells. Importantly, downregulation or silencing of Nanog is promising to be a novel strategy for the treatment of cervical carcinoma.

Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient.

Science.gov (United States)

Liu, Zhong; Zhao, Rui

2016-09-01

Human iPSC line TSD-01-hiPSC was generated from fibroblasts of a patient with infantile Tay-Sachs disease (TSD). The patient is compound heterozygous at the HEXA gene by carrying a 1278insTATC allele and an IVS12+1G>C allele. STEMCCA lentivirus, which expresses OCT4, SOX2, KLF4, and c-MYC from a polycistronic transcript, were used for reprogramming. TSD-01-hiPSC express pluripotency markers such as OCT4, SOX2, NANOG, Tra-1-60, and alkaline phosphatase, and can differentiate into tissues from all the three embryonic germ layers. This TSD patient-derived hiPSC line may serve as a valuable in vitro tool for disease modeling and drug test. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

Protein arginine methyltransferase 7-mediated microRNA-221 repression maintains Oct4, Nanog, and Sox2 levels in mouse embryonic stem cells.

Science.gov (United States)

Chen, Tsai-Yu; Lee, Sung-Hun; Dhar, Shilpa S; Lee, Min Gyu

2018-03-16

The stemness maintenance of embryonic stem cells (ESCs) requires pluripotency transcription factors, including Oct4, Nanog, and Sox2. We have previously reported that protein arginine methyltransferase 7 (PRMT7), an epigenetic modifier, is an essential pluripotency factor that maintains the stemness of mouse ESCs, at least in part, by down-regulating the expression of the anti-stemness microRNA (miRNA) miR-24-2. To gain greater insight into the molecular basis underlying PRMT7-mediated maintenance of mouse ESC stemness, we searched for new PRMT7-down-regulated anti-stemness miRNAs. Here, we show that miR-221 gene-encoded miR-221-3p and miR-221-5p are anti-stemness miRNAs whose expression levels in mouse ESCs are directly repressed by PRMT7. Notably, both miR-221-3p and miR-221-5p targeted the 3' untranslated regions of mRNA transcripts of the major pluripotency factors Oct4, Nanog, and Sox2 to antagonize mouse ESC stemness. Moreover, miR-221-5p silenced also the expression of its own transcriptional repressor PRMT7. Transfection of miR-221-3p and miR-221-5p mimics induced spontaneous differentiation of mouse ESCs. CRISPR-mediated deletion of the miR-221 gene, as well as specific antisense inhibitors of miR-221-3p and miR-221-5p, inhibited the spontaneous differentiation of PRMT7-depleted mouse ESCs. Taken together, these findings reveal that the PRMT7-mediated repression of miR-221-3p and miR-221-5p expression plays a critical role in maintaining mouse ESC stemness. Our results also establish miR-221-3p and miR-221-5p as anti-stemness miRNAs that target Oct4 , Nanog , and Sox2 mRNAs in mouse ESCs. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

Hidroxiapatita e quitosana isoladas e associadas à medula óssea no reparo do tecido ósseo em coelhos. Estudo histológico e morfométrico

Directory of Open Access Journals (Sweden)

Adílio Santos de Azevedo

2013-07-01

Full Text Available O objetivo do presente trabalho foi avaliar o processo de regeneração óssea em coelhos através da utilização da hidroxiapatita e da quitosana isoladas e associadas à medula óssea. Foram utilizados 20 coelhos adultos, de ambos os sexos, da raça Nova Zelândia e pesando em média 3,0±0,4kg. Os animais foram divididos ao acaso de acordo com dois momentos experimentais, sendo que 10 animais foram submetidos à eutanásia 30 dias após a cirurgia, e 10, 60 dias após a cirurgia. Foram utilizados quatro implantes que constituíram os grupos. Os materiais foram introduzidos em defeitos criados cirurgicamente na metáfise proximal (MP e distal (MD de cada fêmur, de modo que a quitosana foi introduzida na MP e a hidroxiapatita na MD do membro esquerdo e, no membro direito, além dos implantes, foi adicionada medula óssea. Na avaliação histológica descritiva comparativa, constatou-se que os grupos QUI e QUIMO, em ambos os momentos estudados, estimularam reações mais acentuadas e aceleraram o reparo ósseo. Na análise morfométrica, os grupos QUI e QUIMO diferiram estatisticamente dos outros grupos, tendo sido observada maior formação óssea (P<0,05.

Transgenic overexpression of NanogP8 in the mouse prostate is insufficient to initiate tumorigenesis but weakly promotes tumor development in the Hi-Myc mouse model.

Science.gov (United States)

Liu, Bigang; Gong, Shuai; Li, Qiuhui; Chen, Xin; Moore, John; Suraneni, Mahipal V; Badeaux, Mark D; Jeter, Collene R; Shen, Jianjun; Mehmood, Rashid; Fan, Qingxia; Tang, Dean G

2017-08-08

This project was undertaken to address a critical cancer biology question: Is overexpression of the pluripotency molecule Nanog sufficient to initiate tumor development in a somatic tissue? Nanog1 is critical for the self-renewal and pluripotency of ES cells, and its retrotransposed homolog, NanogP8 is preferentially expressed in somatic cancer cells. Our work has shown that shRNA-mediated knockdown of NanogP8 in prostate, breast, and colon cancer cells inhibits tumor regeneration whereas inducible overexpression of NanogP8 promotes cancer stem cell phenotypes and properties. To address the key unanswered question whether tissue-specific overexpression of NanogP8 is sufficient to promote tumor development in vivo , we generated a NanogP8 transgenic mouse model, in which the ARR 2 PB promoter was used to drive NanogP8 cDNA. Surprisingly, the ARR 2 PB-NanogP8 transgenic mice were viable, developed normally, and did not form spontaneous tumors in >2 years. Also, both wild type and ARR 2 PB-NanogP8 transgenic mice responded similarly to castration and regeneration and castrated ARR 2 PB-NanogP8 transgenic mice also did not develop tumors. By crossing the ARR 2 PB-NanogP8 transgenic mice with ARR 2 PB-Myc (i.e., Hi-Myc) mice, we found that the double transgenic (i.e., ARR 2 PB-NanogP8; Hi-Myc) mice showed similar tumor incidence and histology to the Hi-Myc mice. Interestingly, however, we observed white dots in the ventral lobes of the double transgenic prostates, which were characterized as overgrown ductules/buds featured by crowded atypical Nanog-expressing luminal cells. Taken together, our present work demonstrates that transgenic overexpression of NanogP8 in the mouse prostate is insufficient to initiate tumorigenesis but weakly promotes tumor development in the Hi-Myc mouse model.

Nano-G accelerometer using geometric anti-springs

NARCIS (Netherlands)

Boom, B. A.; Bertolini, A.; Hennes, E.; Brookhuis, R. A.; Wiegerink, R. J.; Van Den Brand, J. F J; Beker, M. G.; Oner, A.; Van Wees, D.

2017-01-01

We report an ultra-sensitive seismic accelerometer with nano-g sensitivity, using geometric anti-spring technology. High sensitivity is achieved by an on-chip mechanical preloading system comprising four sets of curved leaf springs that support a proof-mass. Using this preloading mechanism,

Resposta osteomielogênica ectópica ao implante de matriz óssea desmineralizada em camundongos tratados com bussulfano

Directory of Open Access Journals (Sweden)

Julieta Rodini Engrácia de Moraes

1996-09-01

Full Text Available Camundongos Swiss, fêmeas, (25-30 g; n = 100 receberam 4 injeções de bussulfano (20 mg/kg, ip a intervalos de 15 dias (n = 100. Antes do tratamento e após 30, 60 e 90 dias, os animais deste e de um grupo controle (n = 15 foram avaliados quanto ao leucograma, taxa de hemoglobina e hematrócito por meio do sangue colhido por punção retroorbital. Sessenta dias após o início do tratamento, 60 animais sobreviventes do grupo tratado e 15 animais do grupo controle receberam implantes intermuseulares de matriz óssea desmineralizada (MOD-10 mg. No 90s dia (30 dias após o implante foram sacrificados para exame histológico da medula óssea esternal (MOE e da resposta osteomielogênica ao implante de MOD. Trinta dias após o início do tratamento observou-se leucopenia, com diminuição do número de granulócitos, linfócitos e monócitos nos animais que receberam bussulfano. O hematócrito e a taxa de hemoglobina apresentaram-se diminuídos no 609 dia. O exame histológico da MOE do grupo tratado revelou hipocelularidade, dilatação dos capilares sinusóides e redução do número de megacariócitos (50%. Os animais controle apresentaram 100% de resposta positiva ao implante de MOD, havendo formação de placas metaplásicas compostas por trabéculas ósseas contendo cavitações preenchidas por células hematopoiéticas. Em 46,7% dos animais tratados observou-se resposta osteomielogênica semelhante à do grupo controle, mas com hipocelularidade e maior componente de adipócitos; 25% apresentaram resposta osteomielogênica tardia e incompleta com menor componente ósseo, maior componente cartilaginoso e ausência de células hematopoiéticas; nos 28,3% restantes a resposta foi negativa.

Generation and periodontal differentiation of human gingival fibroblasts-derived integration-free induced pluripotent stem cells

Energy Technology Data Exchange (ETDEWEB)

Yin, Xiaohui [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China); Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Li, Yang [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Li, Jingwen [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China); Li, Peng [Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR (China); Liu, Yinan [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Wen, Jinhua, E-mail: jhwen@bjmu.edu.cn [Peking University Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191 (China); Luan, Qingxian, E-mail: kqluanqx@126.com [Department of Periodontology, School and Hospital of Stomatology, Peking University, 22 South Avenue Zhong-Guan-Cun, Beijing 100081 (China)

2016-05-06

Induced pluripotent stem cells (iPSCs) have been recognized as a promising cell source for periodontal tissue regeneration. However, the conventional virus-based reprogramming approach is associated with a high risk of genetic mutation and limits their therapeutic utility. Here, we successfully generated iPSCs from readily accessible human gingival fibroblasts (hGFs) through an integration-free and feeder-free approach via delivery of reprogramming factors of Oct4, Sox2, Klf4, L-myc, Lin28 and TP53 shRNA with episomal plasmid vectors. The iPSCs presented similar morphology and proliferation characteristics as embryonic stem cells (ESCs), and expressed pluripotent markers including Oct4, Tra181, Nanog and SSEA-4. Additionally, these cells maintained a normal karyotype and showed decreased CpG methylation ratio in the promoter regions of Oct4 and Nanog. In vivo teratoma formation assay revealed the development of tissues representative of three germ layers, confirming the acquisition of pluripotency. Furthermore, treatment of the iPSCs in vitro with enamel matrix derivative (EMD) or growth/differentiation factor-5 (GDF-5) significantly up-regulated the expression of periodontal tissue markers associated with bone, periodontal ligament and cementum respectively. Taken together, our data demonstrate that hGFs are a valuable cell source for generating integration-free iPSCs, which could be sequentially induced toward periodontal cells under the treatment of EMD and GDF-5. - Highlights: • Integration-free iPSCs are successfully generated from hGFs via an episomal approach. • EMD promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • GDF-5 promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • hGFs-derived iPSCs could be a promising cell source for periodontal regeneration.

Generation and periodontal differentiation of human gingival fibroblasts-derived integration-free induced pluripotent stem cells

International Nuclear Information System (INIS)

Yin, Xiaohui; Li, Yang; Li, Jingwen; Li, Peng; Liu, Yinan; Wen, Jinhua; Luan, Qingxian

2016-01-01

Induced pluripotent stem cells (iPSCs) have been recognized as a promising cell source for periodontal tissue regeneration. However, the conventional virus-based reprogramming approach is associated with a high risk of genetic mutation and limits their therapeutic utility. Here, we successfully generated iPSCs from readily accessible human gingival fibroblasts (hGFs) through an integration-free and feeder-free approach via delivery of reprogramming factors of Oct4, Sox2, Klf4, L-myc, Lin28 and TP53 shRNA with episomal plasmid vectors. The iPSCs presented similar morphology and proliferation characteristics as embryonic stem cells (ESCs), and expressed pluripotent markers including Oct4, Tra181, Nanog and SSEA-4. Additionally, these cells maintained a normal karyotype and showed decreased CpG methylation ratio in the promoter regions of Oct4 and Nanog. In vivo teratoma formation assay revealed the development of tissues representative of three germ layers, confirming the acquisition of pluripotency. Furthermore, treatment of the iPSCs in vitro with enamel matrix derivative (EMD) or growth/differentiation factor-5 (GDF-5) significantly up-regulated the expression of periodontal tissue markers associated with bone, periodontal ligament and cementum respectively. Taken together, our data demonstrate that hGFs are a valuable cell source for generating integration-free iPSCs, which could be sequentially induced toward periodontal cells under the treatment of EMD and GDF-5. - Highlights: • Integration-free iPSCs are successfully generated from hGFs via an episomal approach. • EMD promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • GDF-5 promotes differentiation of the hGFs-derived iPSCs toward periodontal cells. • hGFs-derived iPSCs could be a promising cell source for periodontal regeneration.

Comprometimento da medula óssea e eosinofilia na paracoccidioidomicose

OpenAIRE

Shikanai-Yasuda,M.A.; Higaki,Y.; Uip,D.E.; Mori,N.S.; Del Negro,G.; Melo,N.T.; Hutzler,R.U.; Amato Neto,V.

1992-01-01

São descritos 3 casos de paracoccidioidomicose com a forma aguda da doença, nos quais formas leveduriformes de Paracoccidioides brasiliensis foram visualizadas ao exame direto de medula óssea, sendo a cultura também positiva em um caso. Salienta-se o acometimento do sistema fagocítico-mononuclear e a ausência de resposta às provas cutâneas de hipersensibilidade tardia a antígenos microbianos e de P. brasiliensis em todos, bem como a gravidade do quadro clínico e lesões ósseas generalizadas em...

Sp5 induces the expression of Nanog to maintain mouse embryonic stem cell self-renewal.

Science.gov (United States)

Tang, Ling; Wang, Manman; Liu, Dahai; Gong, Mengting; Ying, Qi-Long; Ye, Shoudong

2017-01-01

Activation of signal transducer and activator of transcription 3 (STAT3) by leukemia inhibitory factor (LIF) maintains mouse embryonic stem cell (mESC) self-renewal. Our previous study showed that trans-acting transcription factor 5 (Sp5), an LIF/STAT3 downstream target, supports mESC self-renewal. However, the mechanism by which Sp5 exerts these effects remains elusive. Here, we found that Nanog is a direct target of Sp5 and mediates the self-renewal-promoting effect of Sp5 in mESCs. Overexpression of Sp5 induced Nanog expression, while knockdown or knockout of Sp5 decreased the Nanog level. Moreover, chromatin immunoprecipitation (ChIP) assays showed that Sp5 directly bound to the Nanog promoter. Functional studies revealed that knockdown of Nanog eliminated the mESC self-renewal-promoting ability of Sp5. Finally, we demonstrated that the self-renewal-promoting function of Sp5 was largely dependent on its zinc finger domains. Taken together, our study provides unrecognized functions of Sp5 in mESCs and will expand our current understanding of the regulation of mESC pluripotency.

Doença óssea em Mieloma Múltiplo Bone disease in Multiple Myeloma

Directory of Open Access Journals (Sweden)

Vania T. M. Hungria

2007-03-01

Full Text Available As principais manifestações clínicas do mieloma múltiplo estão relacionadas à destruição óssea. Esta doença óssea pode levar a fraturas patológicas, compressão da medula espinhal, hipercalcemia e dor, sendo uma das principais causas de morbidade e mortalidade. Estas complicações resultam do desequilíbrio da reabsorção e formação óssea, decorrente do aumento da atividade osteoclástica. Este aumento é mediado pela liberação de fatores ativadores dos osteoclastos, que são produzidos no microambiente da medula óssea por células tumorais e não tumorais. Os bisfosfonatos são inibidores específicos da atividade osteoclástica e são eficazes no tratamento da hipercalcemia associada às neoplasias malignas e podem reduzir o aparecimento de complicações esqueléticas. Estudos recentes identificaram novas moléculas como o receptor de ativação nuclear kappa B (RANK, seu ligante (RANKL, osteoprotegerina (OPG, e a proteína inflamatória dos macrófagos-1alfa, que estão envolvidas na ativação e diferenciação dos osteoclastos, enquanto que a proteína dikkopf-1 inibe a formação óssea osteoblástica. Estas novas moléculas parecem não só interferir na biologia da destruição óssea do mieloma, mas também com a sobrevida e crescimento tumoral, sendo novos alvos para o desenvolvimento de drogas antimieloma. Estudos recentes com anticorpo monoclonal anti-RANKL são promissores. O tratamento da doença óssea do mieloma múltiplo inclui principalmente o uso de bisfosfonatos, radioterapia e procedimentos cirúrgicos.The major clinical manifestation of multiple myeloma is related to osteolytic bone destruction. Bone disease can lead to pathologic fractures, spinal cord compression, hypercalcemia, and pain, and is a major cause of morbidity and mortality. These complications result from asynchronous bone turnover wherein increased osteoclastic bone resorption is not accompanied by a comparable increase in bone formation

Cyclic mechanical strain maintains Nanog expression through PI3K/Akt signaling in mouse embryonic stem cells

International Nuclear Information System (INIS)

Horiuchi, Rie; Akimoto, Takayuki; Hong, Zhang; Ushida, Takashi

2012-01-01

Mechanical strain has been reported to affect the proliferation/differentiation of many cell types; however, the effects of mechanotransduction on self-renewal as well as pluripotency of embryonic stem (ES) cells remains unknown. To investigate the effects of mechanical strain on mouse ES cell fate, we examined the expression of Nanog, which is an essential regulator of self-renewal and pluripotency as well as Nanog-associated intracellular signaling during uniaxial cyclic mechanical strain. The mouse ES cell line, CCE was plated onto elastic membranes, and we applied 10% strain at 0.17 Hz. The expression of Nanog was reduced during ES cell differentiation in response to the withdrawal of leukemia inhibitory factor (LIF); however, two days of cyclic mechanical strain attenuated this reduction of Nanog expression. On the other hand, the cyclic mechanical strain promoted PI3K-Akt signaling, which is reported as an upstream of Nanog transcription. The cyclic mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor wortmannin. Furthermore, cytochalasin D, an inhibitor of actin polymerization, also inhibited the mechanical strain-induced increase in phospho-Akt. These findings imply that mechanical force plays a role in regulating Nanog expression in ES cells through the actin cytoskeleton-PI3K-Akt signaling. -- Highlights: ► The expression of Nanog, which is an essential regulator of “stemness” was reduced during embryonic stem (ES) cell differentiation. ► Cyclic mechanical strain attenuated the reduction of Nanog expression. ► Cyclic mechanical strain promoted PI3K-Akt signaling and mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor and an inhibitor of actin polymerization.

Identification of SSEA-1 expressing enhanced reprogramming (SEER) cells in porcine embryonic fibroblasts

DEFF Research Database (Denmark)

Li, Dong; Secher, Jan Ole Bertelsen; Juhl, Morten

2017-01-01

Previous research has shown that a subpopulation of cells within cultured human dermal fibroblasts, termed multilineage-differentiating stress enduring (Muse) cells, are preferentially reprogrammed into induced pluripotent stem cells. However, controversy exists over whether these cells...... are the only cells capable of being reprogrammed from a heterogeneous population of fibroblasts. Similarly, there is little research to suggest such cells may exist in embryonic tissues or other species. To address if such a cell population exists in pigs, we investigated porcine embryonic fibroblast...... populations (pEFs) and identified heterogeneous expression of several key cell surface markers. Strikingly, we discovered a small population of stage-specific embryonic antigen 1 positive cells (SSEA-1+) in Danish Landrace and Göttingen minipig pEFs, which were absent in the Yucatan pEFs. Furthermore...

A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells.

Science.gov (United States)

Ahmadian Baghbaderani, Behnam; Tian, Xinghui; Scotty Cadet, Jean; Shah, Kevan; Walde, Amy; Tran, Huan; Kovarcik, Don Paul; Clarke, Diana; Fellner, Thomas

2016-01-01

Human pluripotent stem cells (hPSCs) present an unprecedented opportunity to advance human health by offering an alternative and renewable cell resource for cellular therapeutics and regenerative medicine. The present demand for high quality hPSCs for use in both research and clinical studies underscores the need to develop technologies that will simplify the cultivation process and control variability. Here we describe the development of a robust, defined and xeno-free hPSC medium that supports reliable propagation of hPSCs and generation of human induced pluripotent stem cells (hiPSCs) from multiple somatic cell types; long-term serial subculturing of hPSCs with every-other-day (EOD) medium replacement; and banking fully characterized hPSCs. The hPSCs cultured in this medium for over 40 passages are genetically stable, retain high expression levels of the pluripotency markers TRA-1-60, TRA-1-81, Oct-3/4 and SSEA-4, and readily differentiate into ectoderm, mesoderm and endoderm. Importantly, the medium plays an integral role in establishing a cGMP-compliant process for the manufacturing of hiPSCs that can be used for generation of clinically relevant cell types for cell replacement therapy applications.

Níveis séricos de homocisteína em crianças e adolescentes com comprometimento da saúde óssea

Directory of Open Access Journals (Sweden)

Petra Rehackova

2013-12-01

Full Text Available INTRODUÇÃO: Foi documentada uma associação entre níveis séricos elevados de homocisteína (S-Hci e baixa densidade mineral óssea (DMO e aumento do risco de fratura em mulheres na pós-menopausa. São escassos os dados concernentes à S-Hci e à saúde óssea em crianças. OBJETIVO: Avaliar S-Hci em crianças e adolescentes com comprometimento da saúde óssea e procurar por relações com dados clínicos e laboratoriais. PACIENTES E MÉTODOS: Avaliamos os níveis de S-Hci em 37 crianças e adolescentes (22 meninos e 15 meninas; média de idade, 13,9 ± 3,5 anos com fraturas prevalentes por trauma de baixa energia (média 3,3 ± 2,3 por paciente e/ou baixa DMO espinhal/L1-L4 (escore Z abaixo de -2 DP; DXA Lunar GE. Também avaliamos S-ALP, CrossLaps sérico (S-Hci-CrossLaps, osteocalcina (S-OC, altura, peso corporal, índice de massa corporal (IMC e níveis séricos de folato e vitamina B12. Por ocasião da avaliação, as crianças não estavam tomando qualquer medicação que sabidamente influenciasse o metabolismo ósseo. Os parâmetros dependentes de idade foram expressos como escores Z ± DP. RESULTADOS: O escore Z para S-Hci foi significativamente mais alto (1,3 ± 1,5; P < 0,0001 e o escore Z de para DMO/L1-L4 foi significativamente mais baixo (-1,7 ± 1,3; P < 0,0001, respectivamente, em comparação com os valores de referência. S-ALP não diferiu dos valores de referência (P = 0,88, enquanto S-CrossLaps e S-osteocalcina foram mais elevados (1,2 ± 1,8 e 0,4 ± 0,5; P = 0,0001 e P = 0,001, respectivamente. S-Hci estava inversamente correlacionada com DMO/L1-L4 (r = -0,33; P = 0,05 e S-ALP (r = -0,36; P = 0,04 não tendo relação com o número de fraturas prevalentes (r = 0,01, S-osteocalcina (r = -0,22 ou S-CrossLaps (r = 0,003. CONCLUSÃO: Esses resultados sugerem aumento na remodelação óssea e uma influência negativa da S-Hci elevada na formação óssea e na DMO em crianças e adolescentes com fraturas recorrentes.

Cyclic mechanical strain maintains Nanog expression through PI3K/Akt signaling in mouse embryonic stem cells

Energy Technology Data Exchange (ETDEWEB)

Horiuchi, Rie [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Akimoto, Takayuki, E-mail: akimoto@m.u-tokyo.ac.jp [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda-tsurumaki, Shinjuku, Tokyo 162-0041 (Japan); Hong, Zhang [Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda-tsurumaki, Shinjuku, Tokyo 162-0041 (Japan); Ushida, Takashi [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan)

2012-08-15

Mechanical strain has been reported to affect the proliferation/differentiation of many cell types; however, the effects of mechanotransduction on self-renewal as well as pluripotency of embryonic stem (ES) cells remains unknown. To investigate the effects of mechanical strain on mouse ES cell fate, we examined the expression of Nanog, which is an essential regulator of self-renewal and pluripotency as well as Nanog-associated intracellular signaling during uniaxial cyclic mechanical strain. The mouse ES cell line, CCE was plated onto elastic membranes, and we applied 10% strain at 0.17 Hz. The expression of Nanog was reduced during ES cell differentiation in response to the withdrawal of leukemia inhibitory factor (LIF); however, two days of cyclic mechanical strain attenuated this reduction of Nanog expression. On the other hand, the cyclic mechanical strain promoted PI3K-Akt signaling, which is reported as an upstream of Nanog transcription. The cyclic mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor wortmannin. Furthermore, cytochalasin D, an inhibitor of actin polymerization, also inhibited the mechanical strain-induced increase in phospho-Akt. These findings imply that mechanical force plays a role in regulating Nanog expression in ES cells through the actin cytoskeleton-PI3K-Akt signaling. -- Highlights: Black-Right-Pointing-Pointer The expression of Nanog, which is an essential regulator of 'stemness' was reduced during embryonic stem (ES) cell differentiation. Black-Right-Pointing-Pointer Cyclic mechanical strain attenuated the reduction of Nanog expression. Black-Right-Pointing-Pointer Cyclic mechanical strain promoted PI3K-Akt signaling and mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor and an inhibitor of actin polymerization.

[LincRNA-ROR functions as a ceRNA to regulate Oct4, Sox2, and Nanog expression by sponging miR-145 and its effect on biologic characteristics of colonic cancer stem cells].

Science.gov (United States)

Yan, Z Y; Sun, X C

2018-04-08

Objective: To investigate the impact of lincRNA-ROR, a ceRNA by binding miR-145 on the expression of the downstream genes Oct4, Sox2 and Nanog, and related biological characteristics of colon cancer stem cells, and to elucidate the clinical significance of this molecular regulatory network. Methods: Fifty-two cases of colorectal cancer tissue and adjacent tissue were collected at Nanyang City Central Hospital and Nanyang Second Hospital, Henan Province, from 2014 to 2016. Real-time quantitative polymerase chain reaction (qPCR) was used to detect the expression of lincRNA-ROR and miR-145 in colorectal cancer tissue and isolated colon cancer cells. The correlation between the expression of lincRNA-ROR, miR-145 and the clinicopathologic features of colon cancer was performed. CD44(-)CD133(-) and CD44(+) CD133(+) cells were isolated from SW1116 by using flow cytometry. The expression of CD44, CD133, Oct4, Sox2, Nanog, lincRNA-ROR and miR-145 in cells were detected by qPCR. The relationship between lincRNA-ROR, miR-145, Oct4, Sox2 and Nanog was analyzed by bioinformatics, dual luciferase reporter assay, qPCR and Western blot. The effects of silencing lincRNA-ROR on the proliferation and chemosensitivity of colon cancer stem cells were detected by MTT, colony formation. Results: LincRNA-ROR was frequently up-regulated and inversely correlated with miR-145 down-regulation in the colon cancer specimens( P cells were successfully isolated from SW1116 by flow cytometry. The levels of CD44, CD133, Oct4, Sox2, Nanog, lincRNA-ROR in CD44(+) CD133(+) cells were significantly increased, while miR-145 was decreased compared with CD44(-)CD133(-)cells( P cells were significantly reduced upon cell adherence, while miR-145 was significantly increased( P cancer stem cells proliferation and increased the sensitivity to chemotherapy. Conclusions: Linc-ROR functions as a key ceRNA to prevent core TFs, e. g., Oct4, Sox2, Nanog, from miR-145-mediated suppression in colon cancer stem cells

Localization of the stem cell markers LGR5 and Nanog in the normal and the cancerous human ovary and their inter-relationship.

Science.gov (United States)

Amsterdam, Abraham; Raanan, Calanit; Schreiber, Letizia; Freyhan, Ora; Schechtman, Lea; Givol, David

2013-05-01

LGR5 and Nanog were recently characterized as stem cell markers in various embryonic, adult and cancer stem cells. However, there are no data on their precise localization in the normal adult ovary, which may be important for the initial steps of development of ovarian cancer, the most lethal gynecological cancer. We analyzed by immunocytochemistry the precise localization of these markers in normal ovary (11 specimens, age range 43-76), in borderline specimens (12 specimens), and in serous ovarian cancer (12 specimens of stage II) which comprises the vast majority (80%) of all ovarian cancer. Surprisingly, we revealed that both Nanog and LGR5 are clearly localized in the epithelial cells of the normal ovary. However, in 5 of 12 ovaries there was no labeling at all, while in 3 ovaries staining of Nanog was more prominent with only weak labeling of LGR5. In addition, we found in 3 of 11 ovaries clear labeling in foci of both LGR5 and Nanog antibodies, with partial overlapping. Occasionally, we also found in the stroma foci labeled by either Nanog or LGR5 antibodies. In general, the stroma area of tissue sections labeled with LGR5 was much greater than that labeled with Nanog. In borderline tumors a significant portion of the specimens (7 of 12) was labeled exclusively with Nanog and not with LGR5. In ovarian carcinomas almost 100% of the cells were exclusively labeled only with Nanog (6 of 12 of the specimens) with no labeling of LGR5. These data may suggest the potential of ovaries from postmenopausal women, which express Nanog, to undergo transformation, since Nanog was shown to be oncogenic. We conclude that Nanog, which probably plays an important role in ovarian embryonic development, may be partially silenced in fertile and post-menopausal women, but is re-expressed in ovarian cancer, probably by epigenetic activation of Nanog gene expression. Expression of Nanog and LGR5 in normal ovaries and in borderline tumors may assist in the early detection and improved

Perfil sérico da fosfatase alcalina após o reparo de defeitos osteocondrais com células mononucleares da medula óssea e proteína morfogenética óssea em coelhos

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Luiz Augusto Souza

2011-08-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2011v24n4p103 Verificou-se a atividade da fosfatase alcalina sérica em resposta ao reparo de defeitos osteocondrais em vinte e quatro coelhos da raça Nova Zelândia. Distribuídos em três grupos, o controle (GC, o tratado com células mononucleares da medula óssea (GCM e o que recebeu células mononucleares autólogas com proteína morfogenética óssea (GCM+BMP. Após exposição do sulco troclear da articulação fêmoro-tíbio-patelar esquerda removeu-se um segmento em forma de cunha. O local foi preenchido com enxerto osteocondral alógeno conservado em glicerina a 98%. No GC foi realizada a enxertia óssea, no GCM além do enxerto foi inoculado 2x106 células mononucleares e no GCM+BMP foi administrada intra-articularmente células associadas á 1 μg de proteína morfogenética óssea. A resposta osteoblástica foi mensurada por meio da análise sérica de fosfatase alcalina no dia 0 (pré-operatório 3, 15, 30, 45 após cirurgia, além do acompanhamento por exames radiográficos. Procedeu-se a análise de variância em blocos ao acaso, esquema fatorial e teste de Tukey (p=0,05. A média geral do GCM foi superior quando comparada aos outros grupos e as maiores taxas estiveram entre o 15° e 45° dias de pós-operatório. A discrepância dos valores entre os indivíduos do mesmo grupo proporcionam dúvidas na veracidade do teste. Palavras chave: Células-tronco, Consolidação de fraturas, Marcador ósseo

Perfluorooctane sulfonate disturbs Nanog expression through miR-490-3p in mouse embryonic stem cells.

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Bo Xu

Full Text Available Perfluorooctane sulfonate (PFOS poses potential risks to reproduction and development. Mouse embryonic stem cells (mESCs are ideal models for developmental toxicity testing of environmental contaminants in vitro. However, the mechanism by which PFOS affects early embryonic development is still unclear. In this study, mESCs were exposed to PFOS for 24 h, and then general cytotoxicity and pluripotency were evaluated. MTT assay showed that neither PFOS (0.2 µM, 2 µM, 20 µM, and 200 µM nor control medium (0.1% DMSO treatments affected cell viability. Furthermore, there were no significant differences in cell cycle and apoptosis between the PFOS treatment and control groups. However, we found that the mRNA and protein levels of pluripotency markers (Sox2, Nanog in mESCs were significantly decreased following exposure to PFOS for 24 h, while there were no significant changes in the mRNA and protein levels of Oct4. Accordingly, the expression levels of miR-145 and miR-490-3p, which can regulate Sox2 and Nanog expressions were significantly increased. Chrm2, the host gene of miR-490-3p, was positively associated with miR-490-3p expression after PFOS exposure. Dual luciferase reporter assay suggests that miR-490-3p directly targets Nanog. These results suggest that PFOS can disturb the expression of pluripotency factors in mESCs, while miR-145 and miR-490-3p play key roles in modulating this effect.

NANOG Expression as a Responsive Biomarker during Treatment with Hedgehog Signal Inhibitor in Acute Myeloid Leukemia

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Seiji Kakiuchi

2017-02-01

Full Text Available Aberrant activation of the Hedgehog (Hh signaling pathway is involved in the maintenance of leukemic stem cell (LSCs populations. PF-0444913 (PF-913 is a novel inhibitor that selectively targets Smoothened (SMO, which regulates the Hh pathway. Treatment with PF-913 has shown promising results in an early phase study of acute myeloid leukemia (AML. However, a detailed mode of action for PF-913 and relevant biomarkers remain to be elucidated. In this study, we examined bone marrow samples derived from AML patients under PF-913 monotherapy. Gene set enrichment analysis (GSEA revealed that PF-913 treatment affected the self-renewal signature and cell-cycle regulation associated with LSC-like properties. We then focused on the expression of a pluripotency factor, NANOG, because previous reports showed that a downstream effector in the Hh pathway, GLI, directly binds to the NANOG promoter and that the GLI-NANOG axis promotes stemness and growth in several cancers. In this study, we found that a change in NANOG transcripts was closely associated with GLI-target genes and NANOG transcripts can be a responsive biomarker during PF-913 therapy. Additionally, the treatment of AML with PF-913 holds promise, possibly through inducing quiescent leukemia stem cells toward cell cycling.

Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

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Nalini Devi Verusingam

2017-04-01

Full Text Available Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs, reprogramming Oral Squamous Cell Carcinoma (OSCC to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR, immunofluorescence staining, embryoid bodies (EB formation and directed differentiation capacity. Reprogrammed H103 (Rep-H103 exhibited similar ESCs morphologies with flatten cells and clear borders on feeder layer. Reprogrammed H376 (Rep-H376 did not show ESCs morphologies but grow with a disorganized morphology. Critical pluripotency genes Oct4, Sox2 and Nanog were expressed higher in Rep-H103 against the parental counterpart from passage 5 to passage 10. As for Rep-H376, Nanog expression against its parental counterpart showed a significant decrease at passage 5 and although increased in passage 10, the level of expression was similar to the parental cells. Rep-H103 exhibited pluripotent signals (Oct4, Sox2, Nanog and Tra-1-60 and could form EB with the presence of three germ layers markers. Rep-H103 displayed differentiation capacity into adipocytes and osteocytes. The OSCC cell line H103 which was able to be reprogrammed into an iPSC like state showed high expression of Oct4, Sox2 and Nanog at late passage and may provide a potential iPSC model to study multi-stage oncogenesis in OSCC.

LGR5 and Nanog identify stem cell signature of pancreas beta cells which initiate pancreatic cancer.

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Amsterdam, Abraham; Raanan, Calanit; Schreiber, Letizia; Polin, Nava; Givol, David

2013-04-05

Pancreas cancer, is the fourth leading cause of cancer death but its cell of origin is controversial. We compared the localization of stem cells in normal and cancerous pancreas using antibodies to the stem cell markers Nanog and LGR5. Here we show, for the first time, that LGR5 is expressed in normal pancreas, exclusively in the islets of Langerhans and it is co-localized, surprisingly, with Nanog and insulin in clusters of beta cells. In cancerous pancreas Nanog and LGR5 are expressed in the remaining islets and in all ductal cancer cells. We observed insulin staining among the ductal cancer cells, but not in metastases. This indicates that the islet's beta cells, expressing LGR5 and Nanog markers are the initiating cells of pancreas cancer, which migrated from the islets to form the ductal cancerous tissue, probably after mutation and de-differentiation. This discovery may facilitate treatment of this devastating cancer. Copyright © 2013 Elsevier Inc. All rights reserved.

Nanog Fluctuations in Embryonic Stem Cells Highlight the Problem of Measurement in Cell Biology.

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Smith, Rosanna C G; Stumpf, Patrick S; Ridden, Sonya J; Sim, Aaron; Filippi, Sarah; Harrington, Heather A; MacArthur, Ben D

2017-06-20

A number of important pluripotency regulators, including the transcription factor Nanog, are observed to fluctuate stochastically in individual embryonic stem cells. By transiently priming cells for commitment to different lineages, these fluctuations are thought to be important to the maintenance of, and exit from, pluripotency. However, because temporal changes in intracellular protein abundances cannot be measured directly in live cells, fluctuations are typically assessed using genetically engineered reporter cell lines that produce a fluorescent signal as a proxy for protein expression. Here, using a combination of mathematical modeling and experiment, we show that there are unforeseen ways in which widely used reporter strategies can systematically disturb the dynamics they are intended to monitor, sometimes giving profoundly misleading results. In the case of Nanog, we show how genetic reporters can compromise the behavior of important pluripotency-sustaining positive feedback loops, and induce a bifurcation in the underlying dynamics that gives rise to heterogeneous Nanog expression patterns in reporter cell lines that are not representative of the wild-type. These findings help explain the range of published observations of Nanog variability and highlight the problem of measurement in live cells. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

The death-inducer obliterator 1 (Dido1) gene regulates embryonic stem cell self-renewal.

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Liu, Yinyin; Kim, Hyeung; Liang, Jiancong; Lu, Weisi; Ouyang, Bin; Liu, Dan; Songyang, Zhou

2014-02-21

The regulatory network of factors that center on master transcription factors such as Oct4, Nanog, and Sox2 help maintain embryonic stem (ES) cells and ensure their pluripotency. The target genes of these master transcription factors define the ES cell transcriptional landscape. In this study, we report our findings that Dido1, a target of canonical transcription factors such as Oct4, Sox2, and Nanog, plays an important role in regulating ES cell maintenance. We found that depletion of Dido1 in mouse ES cells led to differentiation, and ectopic expression of Dido1 inhibited differentiation induced by leukemia inhibitory factor withdrawal. We further demonstrated that whereas Nanog and Oct4 could occupy the Dido1 locus and promote its transcription, Dido1 could also target to the loci of pluripotency factors such as Nanog and Oct4 and positively regulate their expression. Through this feedback and feedforward loop, Dido1 is able to regulate self-renewal of mouse ES cells.

Different foods in larvicultura of traíra (Hoplias malabaricus

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Samuel Louzada Pereira

2015-10-01

Full Text Available ABSTRACT. Pereira S.L., Mendonça P.P., Pellanda A.S., Matielo M.D. & Gon- çalves Junior L.P. [Different foods in larvicultura of traíra (Hoplias malabaricus.] Utilização de diferentes alimentos na larvicultura de traíra (Hoplias malabaricus. Revista Brasileira de Medicina Veterinária, 37(3:233-238, 2015. Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ 28013-602, Brasil. E-mail: samuell_lp@hotmail.com The feeding in the larviculture of species carnivorous view point for closing of the technological packages of rearing culture. Developed in the sector of Aquicultura of the IFES - Campus of Alegre, this work it aimed at to compare the performance with four diet feed as first exogenous feeding for traíra (Hoplias malabaricus, parameters evaluated: Survival, length final (CF, final weight (PF, tax of specific growth (TCE, development tax specifies (TDE, being put in container with fifteen liters of water, with constant aeration, have five larva of traíra/container. The delineation entire was aleatory with four treatments and five repetitions. Being the diet feeds represented for T1: Nauplios de Artemia sp.; T2: microworm of oats (Pannagrelos redivivus; T3: Wild Zooplâncton; e the inert food represented by T4: Commercial ration in dust with 55% of crude protein and 4,200 Kcal de ED/kg of ration. The water exchange was of 80% daily, the average temperature was of 27.5±1°C. The indices of survival in the T1, T2, T3 and T4, had been 84%, 72%, 100% and 92%, respectively. The date had been submitted analyze it statistics ANOVA and test of Tukey (P<0.01. Wild Zooplâncton was biggest CTF, PF, GP, TCE and TDE. Did not have difference statistics between the Artemia sp. the ration for TCE and TDE. The microworm of oats was minor CF, PF, TCE and TDE. The treatment Wild zooplankton (T3 showed optimum diet food among the other diet feeds tested for the development and

The synergistic effect of 5Azadc and TSA on maintenance of pluripotency of chicken ESCs by overexpression of NANOG gene.

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Wang, Xiaoyan; Wang, Yingjie; Zuo, Qisheng; Li, Dong; Zhang, Wenhui; Lian, Chao; Tang, Beibei; Xiao, Tianrong; Wang, Man; Wang, Kehua; Li, Bichun; Zhang, Yani

2016-04-01

NANOG is a transcription factor that functions in embryonic stem cells (ESCs) and a key factor in maintaining pluripotency. Here, we cloned the NANOG gene promoter from the Rugao yellow chicken and constructed a dual luciferase reporter vector to detect its transcriptional activity and analyze the effects of 5-aza-2'-deoxycytidine (5-Azadc) and trichostatin A (TSA) on NANOG promoter activity and ESC pluripotency maintenance in vitro. NANOG transcriptional activity was enhanced when 5-Azadc and TSA were used alone or together, suggesting the possibility of elevated methylation of the CpG island in the NANOG regulatory region. When ESCs were cultured in basic medium with 5-Azadc and TSA in vitro, significantly more cell colonies were maintained in the 5-Azadc + TSA group than in the control group, which had many differentiated cells and few cell colonies after 6 d of induction. On the tenth day of induction, the cells in the control group fully differentiated and no cell colonies remained, but many cell colonies were present in the 5-Azadc + TSA group. The expression of NANOG in the cell colonies was confirmed by indirect immunofluorescence. Furthermore, ESCs could be passaged to the 12th generation under 5-Azadc and TSA treatment and maintained their pluripotency. Thus, we showed that 5-Azadc and TSA can effectively maintain chicken ESC pluripotency in vitro by increasing NANOG gene expression.

Nano-g Micromachined Inertial Sensors with Low Payload Impact, Phase I

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National Aeronautics and Space Administration — Radiant Acoustics' patented technology for micro-interferometry enables a nano-g intertial sensor for NASA's emerging needs. The proposed sensor system is 1000x more...

Osteopetrose maligna: transplante de medula óssea Malignant osteopetrosis: bone marrow transplantation

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Maria L. Borsato

2008-04-01

Full Text Available A osteopetrose é uma osteopatia hereditária caracterizada pela deficiência na reabsorção óssea que ocorre por disfunção dos osteoclastos. Com o acúmulo de material osteóide que oblitera o canal medular, ocorre hematopoiese extramedular (hepato-esplenomegalia, obliteração dos forames dos nervos cranianos (cegueira, surdez, paralisias faciais, macrocefalia, protusão da fronte, hipertelorismo, exoftalmo, aumento da pressão intracraniana, retardo na erupção dentária, atraso no crescimento, atraso no desenvolvimento neuropsicomotor, e a morte ocorre precocemente nos primeiros anos de vida. A única alternativa terapêutica curativa é o transplante de medula óssea (TMO de doador HLA idêntico, pois restabelece a hematopoiese e a função monócito-macrófago, com melhora das lesões ósseas e anormalidades hematopoiéticas, embora não reverta as alterações sensoriais já instaladas. Os autores relatam casos de duas crianças portadoras de osteopetrose maligna submetidas ao transplante de medula óssea com sucesso. A primeira encontra-se no dia +1260 do TMO, com melhora evidente da radiologia esquelética, sem progressão das deficiências neurológicas que apresentava, e com biópsia óssea sem sinais de osteopetrose. O segundo paciente encontra-se no dia + 700, com sinais de reabsorção óssea e sem progressão dos danos neurológicos. Os autores chamam a atenção para a necessidade de diagnóstico precoce da osteopetrose e o rápido encaminhamento para o transplante de medula óssea antes da instalação de seqüelas neurológicas definitivas.Osteopetrosis is an inherited disorder characterized by the inability to reabsorb and remodel bone due to osteoclast dysfunction. The encroachment by bone and mineralized cartilage of the medullary cavities leads to extramedullary hematopoiesis (hepatosplenomegaly and cranial-nerve foramina leads to blindness, auditory nerve damage, and occulomotor and facial nerve palsies. Defective

Unique Helicase Determinants in the Essential Conjugative TraI Factor from Salmonella enterica Serovar Typhimurium Plasmid pCU1

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McLaughlin, Krystle J.; Nash, Rebekah P.; Redinbo, Mathew R. (UNC)

2014-06-16

The widespread development of multidrug-resistant bacteria is a major health emergency. Conjugative DNA plasmids, which harbor a wide range of antibiotic resistance genes, also encode the protein factors necessary to orchestrate the propagation of plasmid DNA between bacterial cells through conjugative transfer. Successful conjugative DNA transfer depends on key catalytic components to nick one strand of the duplex DNA plasmid and separate the DNA strands while cell-to-cell transfer occurs. The TraI protein from the conjugative Salmonella plasmid pCU1 fulfills these key catalytic roles, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains within a single, 1,078-residue polypeptide. In this work, we unraveled the helicase determinants of Salmonella pCU1 TraI through DNA binding, ATPase, and DNA strand separation assays. TraI binds DNA substrates with high affinity in a manner influenced by nucleic acid length and the presence of a DNA hairpin structure adjacent to the nick site. TraI selectively hydrolyzes ATP, and mutations in conserved helicase motifs eliminate ATPase activity. Surprisingly, the absence of a relatively short (144-residue) domain at the extreme C terminus of the protein severely diminishes ATP-dependent strand separation. Collectively, these data define the helicase motifs of the conjugative factor TraI from Salmonella pCU1 and reveal a previously uncharacterized C-terminal functional domain that uncouples ATP hydrolysis from strand separation activity.

Establishing clonal cell lines with endothelial-like potential from CD9(hi, SSEA-1(- cells in embryonic stem cell-derived embryoid bodies.

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Qizhou Lian

Full Text Available BACKGROUND: Differentiation of embryonic stem cells (ESCs into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage-restricted stem cells has hindered the identification of defining markers for these rare stem cells and, in turn, their isolation. METHODOLOGY/PRINCIPAL FINDINGS: We describe here a method for the isolation of clonal lineage-restricted cell lines with endothelial potential from ESCs through a combination of empirical and rational evidence-based methods. Using an empirical protocol that we have previously developed to generate embryo-derived RoSH lines with endothelial potential, we first generated E-RoSH lines from mouse ESC-derived embryoid bodies (EBs. Despite originating from different mouse strains, RoSH and E- RoSH lines have similar gene expression profiles (r(2 = 0.93 while that between E-RoSH and ESCs was 0.83. In silico gene expression analysis predicted that like RoSH cells, E-RoSH cells have an increased propensity to differentiate into vasculature. Unlike their parental ESCs, E-RoSH cells did not form teratomas and differentiate efficiently into endothelial-like cells in vivo and in vitro. Gene expression and FACS analysis revealed that RoSH and E-RoSH cells are CD9(hi, SSEA-1(- while ESCs are CD9(lo, SSEA-1(+. Isolation of CD9(hi, SSEA-1(- cells that constituted 1%-10% of EB-derived cultures generated an E-RoSH-like culture with an identical E-RoSH-like gene expression profile (r(2 = 0.95 and a propensity to differentiate into endothelial-like cells. CONCLUSIONS: By combining empirical and rational evidence-based methods, we identified definitive selectable surface antigens for the isolation and propagation of lineage-restricted stem cells

Relação cinemática em um trator 4x2 com tração dianteira auxiliar equipado com pneus radiais na eficiência de tração Kinematic relation on radial tires in a front wheel assist tractor on traction efficience

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Mauro Fernando Ferreira

2000-12-01

Full Text Available Diferentes combinações de pressões internas dos pneus do trator pode afetar a interferência entre eixos motrizes dos tratores agrícolas, principalmente com pneus do tipo radial. Um trator 4x2 com tração dianteira auxiliar foi analisado quanto a seu desempenho em tração. Pneus de carcaça radial com diferentes pressões internas foram utilizados, com o objetivo de variar as relações cinemá ticas entre os eixos. Mediram-se o patinamento das rodas dianteiras e traseiras, a resistência ao rolamento e a força de tração, em duas condições de solo (firme e solto. Os resultados obtidos permitiram verificar que a eficiência de tração não foi significativamente influenciada pela variação das relações cinemáticas de 0,962 a 1,102. As máximas eficiências de tração ocorreram com relaçõ es cinemáticas variáveis dentro da faixa estudada e de acordo com as cargas impostas à barra de tração.Different combinations of tractor tire inflating pressure may affect interference between tractor axles, mainly with radial tires type. A front wheel assist tractor was studied in its traction performance. Radial tires with different inflation pressure were used, changing kinematic relations between axles. The measured parameters were: front and rear slip, rolling resistence and drawbar pull in two soil conditions (firm and loose. The results indicate that traction efficience was not significantly influenced by kinematic relations variation between 0.962 to 1.102. The maximum traction efficiency ocurred within the range studied and according to drawbar pull.

A auto-percepção do envelhecimento e os traços de personalidade em idosos

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Yassine, Ismael Macedo Correia

2011-01-01

Tese de mestrado, Psicologia (Secção de Psicologia Clínica e da Saúde - Núcleo de Psicologia Clínica Dinâmica), Universidade de Lisboa, Faculdade de Psicologia, 2011 O presente estudo foca a auto-percepção do envelhecimento e os traços de personalidade, assumindo como principais objectivos: (1) a exploração da relação entre auto-percepção do processo de envelhecimento por parte dos idosos (com mais de 60 anos de idade) e os traços de personalidade e, (2) a compreensão das dimensõe...

Heterogeneity of osteosarcoma cell lines led to variable responses in reprogramming.

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Choong, Pei Feng; Teh, Hui Xin; Teoh, Hoon Koon; Ong, Han Kiat; Choo, Kong Bung; Sugii, Shigeki; Cheong, Soon Keng; Kamarul, Tunku

2014-01-01

Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture.

Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming

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Choong, Pei Feng; Teh, Hui Xin; Teoh, Hoon Koon; Ong, Han Kiat; Choo, Kong Bung; Sugii, Shigeki; Cheong, Soon Keng; Kamarul, Tunku

2014-01-01

Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture. PMID:25170299

MicroRNA-181a* Targets Nanog in a Subpopulation of CD34+ Cells Isolated From Peripheral Blood

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Paul J Mintz

2012-01-01

Full Text Available Exploiting the properties of stem cells by microRNA (miRNA profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC, the targets corresponding to many of these miRNA have not yet been fully elucidated. By miRNA profiling in a subpopulation of CD34+ cells isolated from peripheral blood, we have identified eight clusters of miRNA that were differentially expressed. Further analysis of one of the clusters by bioinformatics revealed that a miRNA, miR-181a*, which is highly expressed in the adherent CD34+ cells, affects the expression levels of Nanog, a stem cell surrogate marker. We show specifically by reporter assay and mutational analysis that miR-181a* targets a seedless 3′ compensatory site in the 3′UTR of Nanog and affects gene expression. We demonstrate that inhibiting miR-181a* upregulates the Nanog expression level, in addition to an increase in alkaline phosphatase activity. Our studies suggest that miR-181a* may be important in controlling the expression level of Nanog in a subpopulation of CD34+ cells.

Legionella dumoffii Tex-KL Mutated in an Operon Homologous to traC-traD is Defective in Epithelial Cell Invasion.

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Qin, Tian; Ken-Ichiro, Iida; Ren, Hong Yu; Zhou, Hai Jian; Yoshida, Shin-Ichi

2016-06-01

To understand the mechanism of invasion by Legionella dumoffii. The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903dIIlacZ. After screening 799 transposon insertion mutants, we isolated one defective mutant. We then constructed the gene-disrupted mutant, KL16, and studied its invasion of and intracellular growth in HeLa and A549 cells, and in A/J mice survival experiments. The structure of traC-traD operon was analyzed by RT-PCR. The transposon insertion was in a gene homologous to Salmonella typhi traC, which is required for the assembly of F pilin into the mature F pilus structure and for conjugal DNA transmission. Results from RT-PCR suggested that the traC-traD region formed an operon. We found that when the traC gene was disrupted, invasion and intracellular growth of L. dumoffii Tex-KL were impaired in human epithelial cells. When mice were infected by intranasal inoculation with a traC deficient mutant, their survival significantly increased when compared to mice infected with the wild-type strain.. Our results indicated that the traC-traD operon is required for the invasion and intracellular growth abilities of L. dumoffii Tex-KL in epithelial cells. Copyright © 2016 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

Changes in stature during and after spinal traction in young male subjects Alterações na estatura antes e após a tração vertebral em homens jovens

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ALF Rodacki

2007-02-01

Full Text Available BACKGROUND: Spinal traction is a relatively popular procedure for increasing the intervertebral space by applying separating forces. The parameters of time and magnitude of the traction forces may influence the outcomes from this procedure and need to be investigated. The duration of the benefits derived from traction is unknown and needs to be determined so that physiotherapists can provide better and more effective treatments. OBJECTIVE: This study analyzed the relationship between load magnitude and time during spinal traction in relation to stature variations. Traction effect duration was also analyzed. METHOD: Fifteen healthy male subjects (23.1 ± 5.77 years; 1.80 ± 0.17 m and 87.0 ± 9.6 kg were assessed under three traction conditions (0, 30 and 60% of body weight, BW of 42 minutes. Stature variation was used to determine intervertebral disc height variation. Stature was assessed every 7 minutes during traction of 42 minutes and every 5 minutes for 45 minutes after traction ceased. RESULTS: 0 and 30% BW traction produced similar gains (6.09 ± 1.89 mm, 5.70 ± 1.88 mm, respectively; p>0.05, while these were smaller (pCONTEXTUALIZAÇÃO: A tração sobre a coluna vertebral é um procedimento relativamente popular para aumentar o espaço inter-vertebral pela aplicação de forças de separação. Os parâmetros de tempo e magnitude da força aplicada podem influenciar os resultados desse procedimento e ainda precisam ser investigados. A duração dos benefícios derivados da tração não é conhecida e precisa ser determinada para que fisioterapeutas possam prover tratamentos melhores e mais eficientes. OBJETIVO: Este estudo analisou a relação entre a magnitude de carga e de tempo durante a tração vertebral sobre as variações de estatura, bem como a duração deste efeito. MÉTODOS: Quinze sujeitos saudáveis do sexo masculino (23,1 ± 5,77 anos; 1,80 ± 0,17 m e 87,0 ± 9,6 Kg foram mensurados sob três condições (0, 30 e 60% PC

Numerical Calculation of Coherent Synchrotron Radiation Effects Using TraFiC4

International Nuclear Information System (INIS)

Kabel, Andreas C.

2000-01-01

Coherent synchrotron radiation (CSR) occurs when short bunches travel on strongly bent trajectories. Its effects on high-quality beams can be severe and are well understood qualitatively. For quantitative results, however, one has to rely on numerical methods. There exist several simulation codes utilizing different approaches. The authors describe in some detail the code TraFiC 4 developed at DESY for design and analysis purposes, which approaches the problem from first principles and solves the equations of motion either perturbatively or self-consistently. They present some calculational results and comparison with experimental data. Also, they give examples of how the code can be used to design beamlines with minimal emittance growth due to CSR

Mineralização óssea em adolescentes do sexo masculino: anos críticos para a aquisição da massa óssea Bone mineralization among male adolescents: critical years for bone mass gain

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Carla C. Silva

2004-12-01

Full Text Available OBJETIVO: Verificar o comportamento do conteúdo mineral ósseo e da densidade mineral óssea em adolescentes do sexo masculino em função da faixa etária e do nível maturacional dos caracteres sexuais secundários. MÉTODOS: 47 adolescentes saudáveis na faixa etária de 10 a 19 anos foram avaliados quanto a ingestão de cálcio, peso, estatura, índice de massa corporal, estágio puberal, densidade mineral óssea e conteúdo mineral ósseo na coluna e no fêmur proximal. A massa óssea foi mensurada através de densitometria óssea. A ingestão de cálcio foi calculada através de registro dietético de 3 dias. O índice de massa corporal foi calculado pelo Índice de Quetelet, e o estágio puberal foi definido segundo os critérios de Tanner. Foi utilizada estatística descritiva, média e desvio padrão, análise de variância para comparação entre os grupos etários e teste de Tukey para localizar as diferenças significativas. RESULTADOS: A ingestão de cálcio não alcançou o valor mínimo de 800 mg em várias faixas etárias estudadas. A densidade mineral óssea e o conteúdo mineral ósseo demonstraram incrementos com o avançar da idade, indicando diferenças significativas a partir dos 14 anos, bem como quando os adolescentes atingiam os estágios de maturação sexual G4. Os parâmetros de mineralização revelaram aumento pronunciado quando os adolescentes atingiam G3, porém sem significado estatístico. CONCLUSÃO: Os resultados apontam um aumento importante na mineralização óssea durante a adolescência. A evidência de aumento na mineralização dos adolescentes foi percebida em níveis maturacionais superiores a G3. Os anos críticos para a aquisição da massa óssea neste estudo evidenciaram-se a partir dos 14-15 anos de idade.OBJECTIVE: To verify the behavior of the mineral bone content and density in male adolescents according to age and secondary sexual characters. METHODS: 47 healthy adolescents between 10 and 19

Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data

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Xin Li

2008-06-01

Full Text Available Abstract Background Target genes of a transcription factor (TF Pou5f1 (Oct3/4 or Oct4, which is essential for pluripotency maintenance and self-renewal of embryonic stem (ES cells, have previously been identified based on their response to Pou5f1 manipulation and occurrence of Chromatin-immunoprecipitation (ChIP-binding sites in promoters. However, many responding genes with binding sites may not be direct targets because response may be mediated by other genes and ChIP-binding site may not be functional in terms of transcription regulation. Results To reduce the number of false positives, we propose to separate responding genes into groups according to direction, magnitude, and time of response, and to apply the false discovery rate (FDR criterion to each group individually. Using this novel algorithm with stringent statistical criteria (FDR Pou5f1 suppression and published ChIP data, we identified 420 tentative target genes (TTGs for Pou5f1. The majority of TTGs (372 were down-regulated after Pou5f1 suppression, indicating that the Pou5f1 functions as an activator of gene expression when it binds to promoters. Interestingly, many activated genes are potent suppressors of transcription, which include polycomb genes, zinc finger TFs, chromatin remodeling factors, and suppressors of signaling. Similar analysis showed that Sox2 and Nanog also function mostly as transcription activators in cooperation with Pou5f1. Conclusion We have identified the most reliable sets of direct target genes for key pluripotency genes – Pou5f1, Sox2, and Nanog, and found that they predominantly function as activators of downstream gene expression. Thus, most genes related to cell differentiation are suppressed indirectly.

The Transit Monitoring in the South (TraMoS project

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López-Morales Mercedes

2013-04-01

Full Text Available We present the Transit Monitoring in the South (TraMoS project. TraMoS has monitored transits of 30 exoplanets with telescopes located in Chile since 2008, whit the following goals: (1 to refine the physical and/or orbital parameters of those exoplanet system, and (2 to search for variations in the mid-times of the transits and in other parameters such as orbital inclination or transit's depth, that could indicate the presence of additional bodies in the system. We highlight here the first results of TraMoS in three selected exoplanets.

In vitro culture of bovine embryos in murine ES cell conditioned media negatively affects expression of pluripotency-related markers OCT4, SOX2 and SSEA1.

Science.gov (United States)

Oliveira, C S; de Souza, M M; Saraiva, N Z; Tetzner, T A D; Lima, M R; Lopes, F L; Garcia, J M

2012-06-01

Despite extensive efforts, establishment of bovine embryonic stem (ES) cell lines has not been successful. We hypothesized that culture conditions for in vitro-produced (IVP) embryos, the most used source of inner cell mass (ICM) to obtain ES cells, might affect their undifferentiated state. Therefore, the aim of this work was to improve pluripotency of IVP blastocysts to produce suitable ICM for further culturing. We tested KSR and foetal calf serum (FCS) supplements in SOF medium and ES cell conditioned medium (CM) on IVC (groups: KSR, KSR CM, FCS and FCS CM). Cleavage and blastocyst rates were similar between all groups. Also, embryonic quality, assessed by apoptosis rates (TUNEL assay), total cell number and ICM percentage did not differ between experimental groups. However, expression of pluripotency-related markers was affected. We detected down-regulation of OCT3/4, SOX2 and SSEA1 in ICM of FCS CM blastocysts (p < 0.05). SOX2 gene expression revealed lower levels (p < 0.05) on KSR CM blastocysts and a remarkable variation in SOX2 mRNA levels on FCS-supplemented blastocysts. In conclusion, pluripotency-related markers tend to decrease after supplementation with ES cell CM, suggesting different mechanisms regulating mouse and bovine pluripotency. KSR supplementation did not differ from FCS, but FCS replacement by KSR may produce blastocysts with stable SOX2 gene expression levels. © 2011 Blackwell Verlag GmbH.

Efeito da desmineralização das superfícies de contato na resistência à tração de enxertos ósseos autógenos em tíbia de coelhos. Analise mecânica e à microscopia eletrônica de varredura

OpenAIRE

Marcus Gustavo Silva Rodrigues

2009-01-01

Vinte e nove coelhos machos (Oryctolagus cuniculus) tiveram fragmentos ósseos corticais de 8mm de diâmetro removidos com trefina da metáfise proximal tibial que foram transplantados para uma área adjacente após a realização de um dos seguintes tratamentos: G-EDTA (desmineralização com EDTA pH neutro a 24% na forma de gel por 3 minutos das superfícies ósseas de contato); G-EDTA-P (o mesmo tratamento de G-EDTA e perfuração do leito receptor); G-P (perfuração do leito receptor) e G-C (nenhum tra...

Expression of Tra2β in Cancer Cells as a Potential Contributory Factor to Neoplasia and Metastasis

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Andrew Best

2013-01-01

Full Text Available The splicing regulator proteins SRSF1 (also known as ASF/SF2 and SRSF3 (also known as SRP20 belong to the SR family of proteins and can be upregulated in cancer. The SRSF1 gene itself is amplified in some cancer cells, and cancer-associated changes in the expression of MYC also increase SRSF1 gene expression. Increased concentrations of SRSF1 protein promote prooncogenic splicing patterns of a number of key regulators of cell growth. Here, we review the evidence that upregulation of the SR-related Tra2β protein might have a similar role in cancer cells. The TRA2B gene encoding Tra2β is amplified in particular tumours including those of the lung, ovary, cervix, stomach, head, and neck. Both TRA2B RNA and Tra2β protein levels are upregulated in breast, cervical, ovarian, and colon cancer, and Tra2β expression is associated with cancer cell survival. The TRA2B gene is a transcriptional target of the protooncogene ETS-1 which might cause higher levels of expression in some cancer cells which express this transcription factor. Known Tra2β splicing targets have important roles in cancer cells, where they affect metastasis, proliferation, and cell survival. Tra2β protein is also known to interact directly with the RBMY protein which is implicated in liver cancer.

miR-150 suppresses the proliferation and tumorigenicity of leukemia stem cells by targeting the Nanog signaling pathway

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Dan-dan Xu

2016-11-01

Full Text Available Proliferation, a key feature of cancer cells, accounts for the majority of cancer-related diseases resulting in mortality. MicroRNAs (miRNAs plays important post-transcriptional modulation roles by acting on multiple signaling pathways, but the underlying mechanism in proliferation and tumorigenicity is unclear. Here, we identified the role of miR-150 in proliferation and tumorigenicity in leukemia stem cells (LSCs (CD34+CD38- cells. miR-150 expression was significantly down-regulated in LSCs from leukemia cell lines and clinical samples. Functional assays demonstrated that increased miR-150 expression inhibited proliferation and clonal and clonogenic growth, enhanced chemosensitivity, and attenuated tumorigenic activity of LSCs in vitro. Transplantation animal studies revealed that miR-150 overexpression progressively abrogates tumour growth. Immunohistochemistry assays demonstrated that miR-150 overexpression enhanced caspase-3 level and reduced Ki-67 level. Moreover, luciferase reporter assays indicated Nanog is a direct and functional target of miR-150. Nanog silencing using small interfering RNA recapitulated anti-proliferation and tumorigenicity inhibition effects. Furthermore, miR-150 directly down-regulated the expression of other cancer stem cell factors including Notch2 and CTNNB1. These results provide insights into the specific biological behaviour of miR-150 in regulating LSC proliferation and tumorigenicity. Targeting this miR-150/Nanog axis would be a helpful therapeutic strategy to treat acute myeloid leukemia.

NANOG reporter cell lines generated by gene targeting in human embryonic stem cells

DEFF Research Database (Denmark)

Fischer, Yvonne; Ganic, Elvira; Ameri, Jacqueline

2010-01-01

Pluripotency and self-renewal of human embryonic stem cells (hESCs) is mediated by a complex interplay between extra- and intracellular signaling pathways, which regulate the expression of pluripotency-specific transcription factors. The homeodomain transcription factor NANOG plays a central role...

Assessment of the Potential of CDK2 Inhibitor NU6140 to Influence the Expression of Pluripotency Markers NANOG, OCT4, and SOX2 in 2102Ep and H9 Cells

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Ade Kallas

2014-01-01

Full Text Available As cyclin-dependent kinases (CDKs regulate cell cycle progression and RNA transcription, CDKs are attractive targets for creating cancer cell treatments. In this study we investigated the effects of the small molecular agent NU6140 (inhibits CDK2 and cyclin A interaction on human embryonic stem (hES cells and embryonal carcinoma-derived (hEC cells via the expression of transcription factors responsible for pluripotency. A multiparameter flow cytometric method was used to follow changes in the expression of NANOG, OCT4, and SOX2 together in single cells. Both hES and hEC cells responded to NU6140 treatment by induced apoptosis and a decreased expression of NANOG, OCT4, and SOX2 in surviving cells. A higher sensitivity to NU6140 application in hES than hEC cells was detected. NU6140 treatment arrested hES and hEC cells in the G2 phase and inhibited entry into the M phase as evidenced by no significant increase in histone 3 phosphorylation. When embryoid bodies (EBs formed from NU6104 treated hES cells were compared to EBs from untreated hES cells differences in ectodermal, endodermal, and mesodermal lineages were found. The results of this study highlight the importance of CDK2 activity in maintaining pluripotency of hES and hEC cells and in differentiation of hES cells.

Erosão na cultura do milho em sucessão à aveia preta e pousio descoberto, em preparo convencional e plantio direto, com tração animal e tratorizada

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R. Levien

2001-09-01

Full Text Available Empregando chuva simulada em Argissolo Vermelho distrófico arênico, com declividade média de 0,12 m m-1, avaliou-se a erosão na cultura do milho (Zea mays L. em preparo convencional e plantio direto, utilizando tração animal e tratorizada, antecedida de pousio descoberto e aveia preta (Avena strigosa Schieb no inverno, no período compreendido entre 1994 e 1995. Chuvas simuladas na intensidade de 64 mm h-1 e duração de 60 a 105 min foram aplicadas em quatro épocas: logo após a semeadura, 30 e 60 dias após a emergência e logo após a colheita do milho. A cobertura do solo propiciada pela cultura de inverno foi reduzida em 90% após o preparo convencional, independentemente da forma de tração. As perdas de solo e água medidas durante o desenvolvimento do milho foram sempre maiores sob preparo convencional do que sob plantio direto, independentemente da forma de tração, exceto logo após a colheita, quando a resteva estava uniformemente distribuída na superfície do solo. No preparo convencional, a erosão na cultura do milho foi, na média das formas de tração, 45% menor, quando antecedida de aveia preta no inverno do que quando antecedida de pousio descoberto. Por sua vez, a cultura do milho, por si só, reduziu a erosão em 60%, na média das formas de tração, comparada à do solo descoberto. A forma de tração influenciou a erosão somente no preparo convencional, com a animal apresentando perdas totais de solo 55 e 15% inferiores à tratorizada, respectivamente na cultura do milho e em solo descoberto. A perda de água por enxurrada foi mais afetada pela forma de tração no preparo convencional, totalizando, em média, 44% na animal e 57% na tratorizada, em relação ao volume total de chuva aplicado.

Effects of Pulsed Electromagnetic Field on Differentiation of HUES-17 Human Embryonic Stem Cell Line

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Yi-Lin Wu

2014-08-01

Full Text Available Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP, stage-specific embryonic antigen-3 (SSEA-3, SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells.

Estudo de novas formulações para regeneração óssea em defeitos de dimensão crítica

OpenAIRE

Palma, Paulo Jorge Rocha da

2009-01-01

Estudo de novas formulações para regeneração óssea em defeitos de dimensão crítica Paulo Jorge Rocha da Palma Coimbra 2009 Resumo: Regenerar o osso perdido ou reabsorvido, é uma das grandes preocupações e desafios do Médico Dentista, necessitando muitas vezes da aplicação de um Biomaterial que permita uma boa regeneração óssea e tecidular. Com o objectivo de promover modalidades biológicas que possam estimular a regeneração óssea, têm vindo a ser desenhadas várias e...

Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM.

Science.gov (United States)

Wetzel, Margaret E; Asenstorfer, Robert E; Tate, Max E; Farrand, Stephen K

2018-04-10

We previously described a plasmid of Agrobacterium spp., pAoF64/95, in which the quorum-sensing system that controls conjugative transfer is induced by the opine mannopine. We also showed that the quorum-sensing regulators TraR, TraM, and TraI function similarly to their counterparts in other repABC plasmids. However, traR, unlike its counterpart on Ti plasmids, is monocistronic and not located in an operon that is inducible by the conjugative opine. Here, we report that both traR and traM are expressed constitutively and not regulated by growth with mannopine. We report two additional regulatory genes, mrtR and tmsP, that are involved in a novel mechanism of control of TraR activity. Both genes are located in the distantly linked region of pAoF64/95 encoding mannopine utilization. MrtR, in the absence of mannopine, represses the four-gene mocC operon as well as tmsP, which is the distal gene of the eight-gene motA operon. As judged by a bacterial two-hybrid analysis, TmsP, which shows amino acid sequence relatedness with the TraM-binding domain of TraR, interacts with the antiactivator. We propose a model in which mannopine, acting through the repressor MrtR, induces expression of TmsP which then titrates the levels of TraM thereby freeing TraR to activate the tra regulon. © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Transient exposure to proteins SOX2, Oct-4, and NANOG immortalizes exhausted tumor-infiltrating CTLs

Energy Technology Data Exchange (ETDEWEB)

Bhadurihauck, Anjuli; Li, Lei [Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, MD (United States); Li, Qianqian; Wang, Jianjun [Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, 48201 (United States); Xiao, Zhengguo, E-mail: xiao0028@umd.edu [Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, MD (United States)

2016-05-13

Adoptive cell transfer therapy (ACT) is one of the most promising immunotherapies against cancer, using tumor-infiltrating lymphocytes (TILs) expanded in vitro. Tumor-infiltrating cytotoxic T lymphocytes (TICTLs) play a prominent role in cancer control. TILs terminally differentiate in response to immunosuppressive environments within tumors, and thus are slow to expand and challenging to maintain both in vitro and in patients. To reverse this exhaustion, we utilize a nuclear protein delivery system that exposes TICTLs to the SOX2, Oct-4, and NANOG (SON) proteins. Unlike activated naïve CTLs (effector CTLs), TICTLs respond favorably to SON treatment, exhibiting steady proliferation and extended survivability independent of cytokine and antigen stimulation. Though TICTLs treated with SON (STICTLs) still express T cell receptors as well as other critical downstream components, they are unresponsive to antigen challenge, suggesting that SON treatment regresses TICTLs into a state similar to that of an early double negative T cell. Our findings indicate the TICTL response to SON proteins is unique when compared to effector CTLs, suggesting TICTLs may be sensitive to regulation by other lineage-specific transcription factors and opening a promising new avenue into cancer immunotherapy. To our knowledge, this is the first report on lineage reprogramming of TILs using protein stem cell transcription factors delivered directly to the nucleus. -- Highlights: •TICTLs are sensitive to reprogramming by proteins of stem cell transcription factors, but effector CTLs were not. •TICTLs are regressed back to an early double negative T cell stage. •TCR signaling is deregulated by these transcription factors.

Generation of human-induced pluripotent stem cells from burn patient-derived skin fibroblasts using a non-integrative method.

Science.gov (United States)

Fu, Shangfeng; Ding, Jianwu; Liu, Dewu; Huang, Heping; Li, Min; Liu, Yang; Tu, Longxiang; Liu, Deming

2018-01-01

Patient specific induced pluripotent stem cells (iPSCs) have been recognized as a possible source of cells for skin tissue engineering. They have the potential to greatly benefit patients with large areas of burned skin or skin defects. However, the integration virus-based reprogramming method is associated with a high risk of genetic mutation and mouse embryonic fibroblast feeder-cells may be a pollutant. In the present study, human skin fibroblasts (HSFs) were successfully harvested from patients with burns and patient-specific iPSCs were generated using a non-integration method with a feeder-free approach. The octamer-binding transcription factor 4 (OCT4), sex-determining region Y box 2 (SOX2) and NANOG transcription factors were delivered using Sendai virus vectors. iPSCs exhibited representative human embryonic stem cell-like morphology and proliferation characteristics. They also expressed pluripotent markers, including OCT4, NANOG, SOX2, TRA181, stage-specific embryonic antigen 4 and TRA-160, and exhibited a normal karyotype. Teratoma and embryoid body formation revealed that iPSCs were able to differentiate into cells of all three germ layers in vitro and in vivo. The results of the present study demonstrate that HSFs derived from patients with burns, may be reprogrammed into stem cells with pluripotency, which provides a basis for cell‑based skin tissue engineering in the future.

Jane Austen: tra brand e desiderio

Directory of Open Access Journals (Sweden)

Donata Meneghelli

2014-12-01

Full Text Available Che Jane Austen, dopo essere giunta a una incontrastata canonizzazione nel pantheon della letteratura occidentale, abbia recentemente intrapreso una   trionfante ascesa nell’ambito della cultura popolare è ormai “una verità universalmente riconosciuta”.   Il saggio analizza i modi, le forme, i supporti e i canali attraverso cui Jane Austen e i suoi testi narrativi (con particolare riferimento a Pride and Prejudice sono usciti dal cerchio tutto sommato ristretto dell’accademia, dei curricola universitari e dei lettori di professione, per entrare in uno spazio che è quello di YouTube, delle fan communities, della musica pop, dei blog, del romanzo rosa o dell’horror; i percorsi grazie ai quali sono diventati parte delle mitologie popolari del XXI secolo e al tempo stesso un brand di successo intorno al quale ruota una fitta rete di interessi economici (industria cinematografica e televisiva, turismo, editoria…, capace di catalizzare (e sfruttare desideri, proiezioni, aspettative di nuove tipologie di lettori; le inedite modalità di ricezione che caratterizzano questo fenomeno, e soprattutto la fan fiction, situata al punto di convergenza o di frizione tra diversi media (letteratura, cinema, televisione, web e media digitali in genere, tra produttori e consumatori, tra lettura e scrittura, tra strategie commerciali delle corporation e autarchia dei fan, tra “alto” e “basso”. Si tratta di un insieme di trasformazioni che scuote alle fondamenta le nostre consuetudini e le nostre credenze in quanto studiosi di letteratura: da una parte, emerge una nuova configurazione della testualità, euforica, rizomatica, molteplice, che si fa beffe di qualunque metafisica dell’originale e realizza in modo perverso alcune celebri utopie critiche; dall’altra, il caso Austen porta alla luce in maniera emblematica le contraddizioni latenti in qualunque processo di democratizzazione della cultura.

Derivation and characterization of novel nonhuman primate embryonic stem cell lines from in vitro-fertilized baboon preimplantation embryos.

Science.gov (United States)

Chang, Tien-Cheng; Liu, Ya-Guang; Eddy, Carlton A; Jacoby, Ethan S; Binkley, Peter A; Brzyski, Robert G; Schenken, Robert S

2011-06-01

The development of nonhuman primate (NHP) embryonic stem cell (ESC) models holds great promise for cell-mediated treatment of debilitating diseases and to address numerous unanswered questions regarding the therapeutic efficacy of ESCs while supplanting ethical considerations involved with human studies. Here we report successful establishment and characterization of 3 novel baboon (Papio cynocephalus) ESC lines from the inner cell mass of intracytoplasmic sperm injection-derived blastocysts. Embryos were cultured in an improved baboon embryo in vitro culture protocol. The inner cell mass of blastocyst was laser-dissected and plated on mouse embryonic fibroblast feeder cell monolayer in the NHP ESC culture medium. Three cell lines with characteristic ESC morphology have been cultured through an extended period (>14 months), with 2 male cell lines (UT-1 and -2) and 1 female cell line (UT-3) displaying normal baboon karyotypes. Reverse transcription-polymerase chain reaction analysis confirmed that all 3 lines express primate ESC pluripotency markers, including OCT-4, NANOG, SOX-2, TERT, TDGF, LEFTYA, and REX-1. All 3 lines demonstrated positive immunocytochemical staining for OCT-4, stage-specific embryonic antigen-3, stage-specific embryonic antigen-4, TRA-1-60, and TRA-1-81. Baboon ESCs injected into NOD/SCID mice formed teratomas with all 3 germ layers. In addition, embryoid body-like spherical structures were derived and initial outgrowth was observed when embedded into extracellular matrix Matrigel. The ESC lines established in this NHP model have the potential to extend our knowledge in the fields of developmental biology, regenerative medicine, and future applications, including preclinical safety assessment of in vivo stem cell therapy.

Chick derived induced pluripotent stem cells by the poly-cistronic transposon with enhanced transcriptional activity.

Science.gov (United States)

Katayama, Masafumi; Hirayama, Takashi; Tani, Tetsuya; Nishimori, Katsuhiko; Onuma, Manabu; Fukuda, Tomokazu

2018-02-01

Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog. The PB-R6F derived iPS cells were alkaline-phosphatase and SSEA-1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three-germ-layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly-cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species. © 2017 Wiley Periodicals, Inc.

Biópsia percutânea de lesões ósseas guiada por tomografia computadorizada: taxas de definição diagnóstica e complicações

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Macello Jose Sampaio Maciel

2014-10-01

Full Text Available Objetivo: Determinar taxas de definição diagnóstica e complicações da biópsia percutânea guiada por tomografia computadorizada (TC de lesões ósseas suspeitas de malignidade. Materiais e Métodos: Estudo retrospectivo que incluiu 186 casos de biópsia percutânea guiada por TC de lesões ósseas no período de janeiro de 2010 a dezembro de 2012. Todas as amostras foram obtidas usando agulhas de 8 a 10 gauge. Foram coletados dados demográficos, história de neoplasia maligna prévia, dados relacionados à lesão, ao procedimento e ao resultado histológico. Resultados: A maioria dos pacientes era do sexo feminino (57% e a idade média foi 53,0 ± 16,4 anos. Em 139 casos (74,6% a suspeita diagnóstica era metástase e os tumores primários mais comuns foram de mama (32,1% e próstata (11,8%. Os ossos mais envolvidos foram coluna vertebral (36,0%, bacia (32,8% e ossos longos (18,3%. Houve complicações em apenas três pacientes (1,6%, incluindo uma fratura, um caso de parestesia com comprometimento funcional e uma quebra da agulha necessitando remoção cirúrgica. Amostras de 183 lesões (98,4% foram consideradas adequadas para diagnóstico. Resultados malignos foram mais frequentes nos pacientes com suspeita de lesão secundária e história de neoplasia maligna conhecida (p < 0,001 e nos procedimentos orientados pela PET/CT (p = 0,011. Conclusão: A biópsia percutânea guiada por TC é segura e eficaz no diagnóstico de lesões ósseas suspeitas.

Influência da suplementação de creatina sobre a massa óssea de ratos espontaneamente hipertensos Influence of creatine supplementation on bone mass of spontaneously hypertensive rats

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Christiano Robles Rodrigues Alves

2012-06-01

Full Text Available INTRODUÇÃO: Recentes evidências indicam que a suplementação de creatina (Cr é capaz de aumentar a densidade mineral óssea (DMO no fêmur de ratos saudáveis em crescimento. Entretanto, há poucos estudos que testam a efetividade da suplementação desse nutriente em condições de perda óssea. OBJETIVO: Investigar o efeito da suplementação de Cr na DMO e no conteúdo mineral ósseo (CMO de ratos espontaneamente hipertensos (SHR, um modelo experimental de baixa massa óssea. MATERIAIS E MÉTODOS: Dezesseis ratos SHR machos com 8 meses de idade foram randomizados em dois grupos experimentais pareados pelo peso corporal, a saber: 1 Pl: SHR tratados com placebo (água destilada; n = 8; e 2 Cr: SHR tratados com Cr (n = 8. Após nove semanas de suplementação os animais foram eutanasiados e o fêmur e a coluna vertebral (L1-L4 foram analisados por densitometria óssea (Dual Energy X-Ray Absorptiometry. RESULTADOS: Não houve diferença significativa na DMO (Pl = 0,249 ± 0,003 g/cm² vs. Cr = 0,249 ± 0,004 g/cm²; P = 0,95 e no CMO (Pl = 0,509 ± 0,150 g vs. Cr = 0,509 ± 0,017 g; P = 0,99 da coluna vertebral e na DMO (Pl = 0,210 ± 0,004 g/cm² vs. Cr = 0,206 ± 0,004 g/cm2;P = 0,49 e no CMO (Pl = 0,407 ± 0,021 g vs. Cr = 0,385 ± 0,021 g; P = 0,46 do fêmur total entre os grupos experimentais. CONCLUSÃO: Neste estudo, usando um modelo experimental de baixa massa óssea, a suplementação de Cr não afetou a massa óssea.INTRODUCTION: Recent evidence has suggested that creatine supplementation (Cr can increase the bone mineral density (BMD of the femur in healthy growing rats. Nevertheless, studies assessing the efficacy of the Cr supplementation in conditions characterized by bone mass loss are scarce. OBJECTIVE: To investigate the effect of Cr supplementation on BMD and bone mineral content (BMC in spontaneously hypertensive rats (SHRs, an experimental model of osteoporosis. MATERIALS AND METHODS: Sixteen 8-month-old male SHRs were

Production and characterization of monoclonal antibodies specific to pangasius catfish, basa, and tra.

Science.gov (United States)

Gajewski, K G; Chen, Y-T; Hsieh, Y-H P

2009-04-01

Four IgG (subclass IgG1) class monoclonal antibodies (MAbs) strongly reactive to Asian farm-raised Pangasius catfish, tra (Pangasius hypophthalmus) and basa (Pangasius bocourti), have been developed. These MAbs were raised by immunizing an animal with thermal-stable crude sarcoplasmic protein extract of cooked tra. The MAbs were selected by screening hybridoma clones against more than 70 common fish and meat protein extracts. Two MAbs, T7E10 and T1G11, were found to be specific to the Asian Pangasius catfish, tra, and basa, with no cross-reactions with any of the common fish and meat species or with the food additive proteins (bovine serum albumin, soy proteins, milk proteins, egg proteins, and gelatin) tested. MAb T7E10 recognized 2 antigenic proteins (molecular weight approximately 36 and 75 kDa) in raw and cooked tra and basa extracts, while T1G11 bound to several proteins (molecular weight between 13 and 18 kDa) in tra and basa extracts. Two other MAbs, F7B8 and F1G11, recognized a common protein (36 KDa) and cross-reacted with all the fish extracts tested and with several mammalian species. These MAbs can be employed individually or in combination in various formats of immunoassays for rapid identification of Pangasius catfish, either raw or cooked. They can also be used to study the biological, biochemical, and physiological aspects of thermal-stable antigenic proteins. This is the first study identifying these thermal-stable antigenic proteins present in Pangasius catfish as species-specific biomarkers.

Torque nos rodados motrizes de um trator agrícola submetido a ensaios de tração

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Diego A. Fiorese

2015-09-01

Full Text Available RESUMOA determinação do torque nos rodados motrizes dos tratores agrícolas complementa os ensaios de tração e permite o cálculo da distribuição dos esforços tratórios entre os eixos. O objetivo principal desta pesquisa foi determinar a demanda de torque nos rodados motrizes de um trator com potência do motor de 89 kW, buscando avaliar o torque demandado em função da utilização da tração dianteira assistida (TDA, para distintas marchas e conforme aumento da força de tração. Foram realizados ensaios de tração sobre pista de concreto utilizando-se um torciômetro instalado no eixo traseiro do trator, um carro dinamométrico e instrumentação eletrônica para aquisição de dados. Avaliaram-se a TDA ligada e desligada, quatro marchas (5, 7, 8 e 9 km h-1 e oito cargas na barra de tração (4, 8, 12, 16, 20, 24, 28, 30 kN. Os resultados analisados estatisticamente através de equações de regressão indicaram comportamento similar para todas as marchas sendo que, conforme o aumento da força de tração ocorreu crescimento linear da demanda de torque nos eixos motrizes. Com a TDA ligada nas cargas mais baixas o torque é maior no eixo dianteiro e nas cargas mais altas é maior no eixo traseiro.

Níveis s��ricos de vitamina B12 não se relacionam com baixa densidade mineral óssea em mulheres brasileiras na pós-menopausa Serum levels of vitamin B12 are not related to low bone mineral density in postmenopausal Brazilian women

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Adriana Maria Kakehasi

2012-12-01

Full Text Available INTRODUÇÃO: Osteoporose e deficiência de vitamina B12 são condições de prevalência crescente com o envelhecimento. A associação entre níveis séricos de vitamina B12, baixa massa óssea e risco aumentado de fraturas ósseas tem sido descrita, mas os estudos não são homogêneos e os resultados são controversos. OBJETIVO: Investigar a associação entre níveis plasmáticos de vitamina B12 e densidade mineral óssea em mulheres assintomáticas na pós-menopausa. MÉTODOS: Mulheres assintomáticas no período pós-menopausa foram consecutivamente incluídas neste estudo transversal. A densidade mineral óssea (coluna lombar e fêmur foi avaliada pelo método DXA Lunar Prodigy Vision, e foram determinados níveis sanguíneos de vitamina B12, cálcio, fósforo, fosfatase alcalina óssea e hormônio paratireoidiano. Os critérios da Organização Mundial de Saúde foram considerados para o diagnóstico de osteoporose. RESULTADOS: Foram incluídas neste estudo 70 mulheres, com média de idade de 62,5 ± 7 anos. Do total, 18 (25,7% apresentaram densidade mineral óssea normal, 33 (47,1% tinham osteopenia e 19 (27,1% tinham osteoporose. Seis (8,6% pacientes haviam apresentado fratura de punho, duas (2,8% reportaram diagnóstico de fratura vertebral e apenas uma (1,4% paciente havia sofrido fratura de quadril. Os níveis de vitamina B12 (média ± DP; pg/mL das mulheres com densidade mineral óssea normal, osteopenia e osteoporose foram 590,2 ± 364,3, 536,6 ± 452,3 e 590,2 ± 497,9, respectivamente (P = 0,881. A análise de regressão múltipla mostrou que o índice de massa corporal e a fosfatase alcalina óssea foram preditores da densidade mineral óssea na coluna lombar. CONCLUSÃO: Os resultados indicam que níveis de vitamina B12 não se relacionam à densidade mineral óssea neste grupo de mulheres brasileiras na pós-menopausa.INTRODUCTION: Osteoporosis and vitamin B12 deficiency are conditions with an increasing prevalence over

Investigation of antioxidant potential of peptide fractions from the Tra Catfish by-product-derived hydrolysate using Alcalase® 2.4 L FG

Science.gov (United States)

Vo, Tam D. L.; Chung, Duy T. M.; Doan, Kien T.; Le, Duy T.; Trinh, Hung V.

2017-09-01

In this study, the antioxidant capacity of peptide fractions isolated from the Tra Catfish (Pangasius hypophthalmus) by-product-derived proteolysate using ultrafiltration centrifugal devices with 5 distinct molecular-weight cutoffs (MWCOs) of 1 kDa, 3 kDa, 5 kDa, 10 kDa, and 30 kDa was investigated. Firstly, the chemical composition of the Tra Catfish by-products was analyzed. The result showed that the Tra Catfish by-products contained 58.5% moisture, 33.9% crude protein, 50.1% crude lipid and 15.8% ash (on dry weight basis). Secondly, the effects of hydrolysis time, enzyme content on the antioxidant potential of the proteolysate were studied using DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method (DPPH• SM) and FRAP (Ferric Reducing Antioxidant Potential) method. Alcalase® 2.4 L FG was used for hydrolysis. The result of antioxidant activity of the hydrolysate showed that the 50% DPPH• inhibition concentration (IC50) of the hydrolysate reached about 6775 µg/mL which was 1645-fold higher than that of vitamin C and 17-fold higher than that of BHT (ButylatedHydroxytoluene) with the degree of hydrolysis (DH) of the hydrolysate of 14.6% when hydrolysis time was 5 hours, enzyme/substrate (E/S) ratio was 30 U/g protein, hydrolysis temperature was 55°C, and pH was 7.5. The antioxidant potential of hydrolysate using FRAP method reached about 52.12 µM Trolox equivalent which was 53-fold and 18-fold lower than those of vitamin C and BHT, respectively, when the hydrolysis time was 5 h, enzyme/substrate ratio was 30 U/g protein, temperature was 500C, and pH level was 8. Next, the proteolysate was further fractionated using MWCOs of 1 kDa, 3 kDa, 5 kDa, 10 kDa, and 30 kDa and the peptide fractions were investigated for their antioxidant activity. The result showed that the <1 kDa fraction showed strongest antioxidant activity with the IC50 of 1313.31 ± 50.65 µg/mL and FRAP value of 906.90 ± 44.32 µM Trolox equivalent. The second strongest fraction

Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development.

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Sushma Grellscheid

2011-12-01

Full Text Available Alternative splicing amplifies the information content of the genome, creating multiple mRNA isoforms from single genes. The evolutionarily conserved splicing activator Tra2β (Sfrs10 is essential for mouse embryogenesis and implicated in spermatogenesis. Here we find that Tra2β is up-regulated as the mitotic stem cell containing population of male germ cells differentiate into meiotic and post-meiotic cells. Using CLIP coupled to deep sequencing, we found that Tra2β binds a high frequency of exons and identified specific G/A rich motifs as frequent targets. Significantly, for the first time we have analysed the splicing effect of Sfrs10 depletion in vivo by generating a conditional neuronal-specific Sfrs10 knock-out mouse (Sfrs10(fl/fl; Nestin-Cre(tg/+. This mouse has defects in brain development and allowed correlation of genuine physiologically Tra2β regulated exons. These belonged to a novel class which were longer than average size and importantly needed multiple cooperative Tra2β binding sites for efficient splicing activation, thus explaining the observed splicing defects in the knockout mice. Regulated exons included a cassette exon which produces a meiotic isoform of the Nasp histone chaperone that helps monitor DNA double-strand breaks. We also found a previously uncharacterised poison exon identifying a new pathway of feedback control between vertebrate Tra2 proteins. Both Nasp-T and the Tra2a poison exon are evolutionarily conserved, suggesting they might control fundamental developmental processes. Tra2β protein isoforms lacking the RRM were able to activate specific target exons indicating an additional functional role as a splicing co-activator. Significantly the N-terminal RS1 domain conserved between flies and humans was essential for the splicing activator function of Tra2β. Versions of Tra2β lacking this N-terminal RS1 domain potently repressed the same target exons activated by full-length Tra2β protein.

KLF4 Nuclear Export Requires ERK Activation and Initiates Exit from Naive Pluripotency.

Science.gov (United States)

Dhaliwal, Navroop K; Miri, Kamelia; Davidson, Scott; Tamim El Jarkass, Hala; Mitchell, Jennifer A

2018-04-10

Cooperative action of a transcription factor complex containing OCT4, SOX2, NANOG, and KLF4 maintains the naive pluripotent state; however, less is known about the mechanisms that disrupt this complex, initiating exit from pluripotency. We show that, as embryonic stem cells (ESCs) exit pluripotency, KLF4 protein is exported from the nucleus causing rapid decline in Nanog and Klf4 transcription; as a result, KLF4 is the first pluripotency transcription factor removed from transcription-associated complexes during differentiation. KLF4 nuclear export requires ERK activation, and phosphorylation of KLF4 by ERK initiates interaction of KLF4 with nuclear export factor XPO1, leading to KLF4 export. Mutation of the ERK phosphorylation site in KLF4 (S132) blocks KLF4 nuclear export, the decline in Nanog, Klf4, and Sox2 mRNA, and differentiation. These findings demonstrate that relocalization of KLF4 to the cytoplasm is a critical first step in exit from the naive pluripotent state and initiation of ESC differentiation. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

Evidências de Anomalias Ósseas em Stahleckeria potens Huene, 1935 (Therapsida, Anomodontia.

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Cibele Schwanke

2007-07-01

Full Text Available Os dicinodontes representam um grupo de tetrápodes herbívoros amplamente registrado em rochas sedimentares do Permiano Superior eTriássico. Uma das espécies mais instigantes é Stahleckeria potens Huene 1935, uma forma de porte avantajado cujos fósseis, até o momento, foram registrados unicamente em sedimentos mesotriássicos da Formação Santa Maria (Bacia do Paraná, Brasil. Nos últimos anos, novos materiais atribuídos a S. potens têm sido identificadosaumentando consideravelmente o conhecimento da morfologia da espécie. A análise comparativa de dois fêmures esquerdos depositados no Museu de Ciências da Terra (DNPM/RJ evidenciam anormalidadesósseas significativas (DGM 155-R e DGM- 399-R, aqui apresentadas preliminarmente e sugestivamente associadas a patologias. O espécime DGM 399- R caracteriza-se por apresentar uma acentuada depressão na região da epífise distal. Esta depressão localiza-se na face anterior do osso, lateralmente posicionada e possui um formato grosseiramente circular, criando uma espécie de platô nesta região,acompanhada por um leve deslocamento ósseo. Em oposição, na face posterior do osso, há um pequeno sulco de formato claramente semicircular, onde são visualizadas cerca de sete pequenas perfurações rasas, que demonstram certa regularidade, porémsem formato definido. Tais feições sugerem que as alterações ósseas presentes no espécime possam estar associadas a lesões ou traumas decorrentes de diversos fatores. Embora não observado claramente,há evidências de cicatrização local, hipótese que vem sendo analisada mais detalhadamente a partir da utilização de técnicas de maior precisão e resolução, como tomografia computadorizada e microscopia eletrônica de varredura. A ocorrência de uma extensaregião marcada pela presença de estrutura óssea irregular, diferente do padrão normal linear, pode ser interpretada como decorrente de remodelação do periósteo, resultante de um

Crystal structures of the F and pSLT plasmid TraJ N-terminal regions reveal similar homodimeric PAS folds with functional interchangeability

Energy Technology Data Exchange (ETDEWEB)

Lu, Jun; Wu, Ruiying; Adkins, Joshua N.; Joachimiak, Andrzej; Glover, Mark

2014-09-16

In the F-family of conjugative plasmids, TraJ is an essential transcriptional activator of the tra operon that encodes most of the proteins required for conjugation. Here we report for the first time the X-ray crystal structures of the TraJ N-terminal regions from the prototypic F plasmid (TraJF11-130) and from the Salmonella virulence plasmid pSLT (TraJpSLT 1-128). Both proteins form similar homodimeric Per-ARNT-Sim (PAS) fold structures. Mutational analysis reveals that the observed dimeric interface is critical for TraJF transcriptional activation, indicating that dimerization of TraJ is required for its in vivo function. An artificial ligand (oxidized dithiothreitol) occupies a cavity in the TraJF dimer interface, while a smaller cavity in corresponding region of the TraJpSLT structure lacks a ligand. Gas chromatography/mass spectrometry-electron ionization analysis of dithiothreitol-free TraJF suggests indole may be the natural TraJ ligand; however, disruption of the indole biosynthetic pathway does not affect TraJF function. Heterologous PAS domains from pSLT and R100 TraJ can functionally replace the TraJF PAS domain, suggesting that TraJ allelic specificity is mediated by the region C-terminal to the PAS domain.

Stochastic NANOG fluctuations allow mouse embryonic stem cells to explore pluripotency

Czech Academy of Sciences Publication Activity Database

Abranches, E.; Guedes, A.M.V.; Moravec, Martin; Maamar, H.; Svoboda, Petr; Raj, A.; Henrique, D.

2014-01-01

Roč. 141, č. 14 (2014), s. 2770-2779 ISSN 0950-1991 R&D Projects: GA ČR(CZ) GBP305/12/G034 Grant - others:Fundacao para a Ciencia e Tecnologia(PT) SFRH/ BPD/78313/2011; Fundacao para a Ciencia e Tecnologia(PT) SFRH/BD/80191/2011; Fundacao para a Ciencia e Tecnologia(PT) PTDC/SAUOBD/100664/2008; GA AV ČR M200521202 Institutional support: RVO:68378050 Keywords : Gene expression heterogeneity * Pluripotency * Lineage priming * Nanog * Stem cells Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 6.462, year: 2014

Upregulation of mitochondrial NAD+ levels impairs the clonogenicity of SSEA1+ glioblastoma tumor-initiating cells.

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Son, Myung Jin; Ryu, Jae-Sung; Kim, Jae Yun; Kwon, Youjeong; Chung, Kyung-Sook; Mun, Seon Ju; Cho, Yee Sook

2017-06-09

Emerging evidence has emphasized the importance of cancer therapies targeting an abnormal metabolic state of tumor-initiating cells (TICs) in which they retain stem cell-like phenotypes and nicotinamide adenine dinucleotide (NAD + ) metabolism. However, the functional role of NAD + metabolism in regulating the characteristics of TICs is not known. In this study, we provide evidence that the mitochondrial NAD + levels affect the characteristics of glioma-driven SSEA1 + TICs, including clonogenic growth potential. An increase in the mitochondrial NAD + levels by the overexpression of the mitochondrial enzyme nicotinamide nucleotide transhydrogenase (NNT) significantly suppressed the sphere-forming ability and induced differentiation of TICs, suggesting a loss of the characteristics of TICs. In addition, increased SIRT3 activity and reduced lactate production, which are mainly observed in healthy and young cells, appeared following NNT-overexpressed TICs. Moreover, in vivo tumorigenic potential was substantially abolished by NNT overexpression. Conversely, the short interfering RNA-mediated knockdown of NNT facilitated the maintenance of TIC characteristics, as evidenced by the increased numbers of large tumor spheres and in vivo tumorigenic potential. Our results demonstrated that targeting the maintenance of healthy mitochondria with increased mitochondrial NAD + levels and SIRT3 activity could be a promising strategy for abolishing the development of TICs as a new therapeutic approach to treating aging-associated tumors.

Antagonism between the transcription factors NANOG and OTX2 specifies rostral or caudal cell fate during neural patterning transition.

Science.gov (United States)

Su, Zhenghui; Zhang, Yanqi; Liao, Baojian; Zhong, Xiaofen; Chen, Xin; Wang, Haitao; Guo, Yiping; Shan, Yongli; Wang, Lihui; Pan, Guangjin

2018-03-23

During neurogenesis, neural patterning is a critical step during which neural progenitor cells differentiate into neurons with distinct functions. However, the molecular determinants that regulate neural patterning remain poorly understood. Here we optimized the "dual SMAD inhibition" method to specifically promote differentiation of human pluripotent stem cells (hPSCs) into forebrain and hindbrain neural progenitor cells along the rostral-caudal axis. We report that neural patterning determination occurs at the very early stage in this differentiation. Undifferentiated hPSCs expressed basal levels of the transcription factor orthodenticle homeobox 2 (OTX2) that dominantly drove hPSCs into the "default" rostral fate at the beginning of differentiation. Inhibition of glycogen synthase kinase 3β (GSK3β) through CHIR99021 application sustained transient expression of the transcription factor NANOG at early differentiation stages through Wnt signaling. Wnt signaling and NANOG antagonized OTX2 and, in the later stages of differentiation, switched the default rostral cell fate to the caudal one. Our findings have uncovered a mutual antagonism between NANOG and OTX2 underlying cell fate decisions during neural patterning, critical for the regulation of early neural development in humans. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

Bases ósseas e musculares dos cortes comerciais do tronco de jacaré-do-Pantanal (Caimanyacare Daudin, 1802

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Sandra I.S. Figueiredo

2015-08-01

Full Text Available Resumo: O consumo de carne de jacaré-do-Pantanal tornou-se uma tendência de mercado e uma cadeia produtiva em ascensão no Estado de Mato Grosso, sendo 28,40% da carne comercializada nos últimos quatro anos oriundos do tronco. Estudos evolutivos, morfofisiológicos, ontogenéticos e tecnológicos foram desenvolvidos, mas não há descrição da musculatura e bases ósseas dos cortes comerciais. Objetivou-se descrever os músculos e correspondentes bases ósseas dos cortes filé de lombo, filé mignon e aparas. Na descrição óssea, utilizaram-se seis carcaças desossadas de exemplares juvenis de jacaré-do-Pantanal, além de um exemplar adulto, obtido por doação após óbito, do Zoológico da UFMT. Os ossos foram macerados em água corrente, clareados e descritos. Para a descrição muscular, 24 exemplares juvenis foram abatidos e esfolados, conservados em freezer e descongelados quando utilizados, sem qualquer fixação. Após a evisceração, foram dissecados em ambos os antímeros. Os músculos semiespinhal, longuíssimo e iliocostal, fixados nas vértebras e costelas torácicas, lombares e sacrais, formam o filé de lombo. O corte aparas é constituído pelos músculos grande dorsal, serrátil, peitoral e abdominais (oblíquo externo, oblíquo interno, transverso e reto, cuja base óssea corresponde as costelas torácicas, lombares e sacrais, a gastrália, o esterno e o epipúbis. Por sua vez, o m. puboisquiofemoral interno cranial, localizado na região sublombar e o m. troncocaudal, da superfície ventral da pelve, compreendem o filé mignon.

Nanostructured graphite-induced destabilization of LiBH4 for reversible hydrogen storage

CSIR Research Space (South Africa)

Wang, K

2016-11-01

Full Text Available been conducted to gain insight into the promoting effect of nano-G on the reversible dehydrogenation of the LiBH(sub4). Our study found that nano-G exerts its promoting effect via interaction with LiBH(sub4) and as grinding aid....

45 CFR 60.4 - How information must be reported.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false How information must be reported. 60.4 Section 60.4 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION NATIONAL PRACTITIONER DATA BANK FOR ADVERSE INFORMATION ON PHYSICIANS AND OTHER HEALTH CARE PRACTITIONERS Reporting of...

A Ligadura Tubária é Fator de Risco para a Redução da Densidade Mineral Óssea em Mulheres na Pós-menopausa?

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Zabaglia Silval Fernando Cardoso

2001-01-01

Full Text Available Objetivo: comparar a densidade mineral óssea de mulheres menopausadas com e sem o antecedente de laqueadura tubária e avaliar quais fatores nos dois grupos poderiam estar associados à densidade mineral óssea no fêmur e coluna lombar. Métodos: foram incluídas 70 pacientes em cada grupo, no ano de 1998, do Centro de Atenção Integral à Saúde da Mulher da Universidade Estadual de Campinas. As mulheres responderam um questionário sobre características clínicas e reprodutivas e foram submetidas à densitometria óssea (Lunar DPX no fêmur e coluna lombar. Os dados foram analisados por meio dos testes t de Student, exato de Fisher, c² de Pearson, t com correção de Bonferroni e regressão múltipla. Resultados: a média etária das laqueadas foi de 53,2 anos e das não-laqueadas de 52,6 anos, com média da idade à menopausa de 48 anos, semelhante nos dois grupos. As médias de idade à cirurgia foi 33,7 anos, com tempo decorrido da cirurgia de 18 anos. A média da densidade mineral óssea para o fêmur e coluna lombar não apresentaram diferenças significativas entre os dois grupos. A distribuição percentual em categorias de T-score do fêmur e coluna lombar não mostrou diferenças significativas nos dois grupos. Na regressão múltipla, observou-se que a idade mostrou uma associação inversa e o índice de massa corporal uma associação direta com a densidade mineral óssea no fêmur. Para a coluna lombar, cor não-branca, paridade, idade à menopausa, escolaridade e índice de massa corporal mostraram uma associação direta e a idade à menarca uma associação inversa com a densidade mineral óssea. Conclusão: a laqueadura tubária não ocasionou redução na massa óssea em mulheres na pós-menopausa.

I varchi della letteratura: tra scienza, follia e spiritualità

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Stefano Redaelli

2017-08-01

Full Text Available Nel novecento italiano spiccano scrittori nella cui opera la letteratura si è aperta ad altre discipline, come la scienza, la psichiatria, la teologia. In questo articolo si descrive lo scambio tra saperi attraverso l’immagine dei varchi, portando l’esempio di Primo Levi (nel varco tra letteratura e scienza e Alda Merini (nel varco tra letteratura, follia, spiritualità. Se da una parte nella vita degli scrittori il varco è vissuto come spaccatura, dall’altra troviamo nella loro opera una fusione di saperi e linguaggi diversi, che concorrono a una rappresentazione più ampia della natura e dell’uomo.

Genomic and expression analyses of Tursiops truncatus T cell receptor gamma (TRG) and alpha/delta (TRA/TRD) loci reveal a similar basic public γδ repertoire in dolphin and human.

Science.gov (United States)

Linguiti, Giovanna; Antonacci, Rachele; Tasco, Gianluca; Grande, Francesco; Casadio, Rita; Massari, Serafina; Castelli, Vito; Consiglio, Arianna; Lefranc, Marie-Paule; Ciccarese, Salvatrice

2016-08-15

The bottlenose dolphin (Tursiops truncatus) is a mammal that belongs to the Cetartiodactyla and have lived in marine ecosystems for nearly 60 millions years. Despite its popularity, our knowledge about its adaptive immunity and evolution is very limited. Furthermore, nothing is known about the genomics and evolution of dolphin antigen receptor immunity. Here we report a evolutionary and expression study of Tursiops truncatus T cell receptor gamma (TRG) and alpha/delta (TRA/TRD) genes. We have identified in silico the TRG and TRA/TRD genes and analyzed the relevant mature transcripts in blood and in skin from four subjects. The dolphin TRG locus is the smallest and simplest of all mammalian loci as yet studied. It shows a genomic organization comprising two variable (V1 and V2), three joining (J1, J2 and J3) and a single constant (C), genes. Despite the fragmented nature of the genome assemblies, we deduced the TRA/TRD locus organization, with the recent TRDV1 subgroup genes duplications, as it is expected in artiodactyls. Expression analysis from blood of a subject allowed us to assign unambiguously eight TRAV genes to those annotated in the genomic sequence and to twelve new genes, belonging to five different subgroups. All transcripts were productive and no relevant biases towards TRAV-J rearrangements are observed. Blood and skin from four unrelated subjects expression data provide evidence for an unusual ratio of productive/unproductive transcripts which arise from the TRG V-J gene rearrangement and for a "public" gamma delta TR repertoire. The productive cDNA sequences, shared both in the same and in different individuals, include biases of the TRGV1 and TRGJ2 genes. The high frequency of TRGV1-J2/TRDV1- D1-J4 productive rearrangements in dolphins may represent an interesting oligo-clonal population comparable to that found in human with the TRGV9- JP/TRDV2-D-J T cells and in primates. Although the features of the TRG and TRA/TRD loci organization reflect

Potencial evocado auditivo de tronco encefálico por frequência específica por via aérea e via óssea em neonatos ouvintes normais

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Natália Ramos

2014-06-01

Full Text Available Objetivo determinar os níveis mínimos de resposta (NMR e a latência da onda V do Potencial Evocado Auditivo de Tronco encefálico por Frequência específica (PEATE-FE em neonatos ouvintes normais nas frequências de 0.5, 1, 2 e 4 kHz por via aérea e via óssea e determinar valores normativos. Métodos foram avaliados neonatos com audição normal, sendo realizado o PEATE-FE nas frequências de 0.5, 1, 2 e 4 kHz, tanto por via aérea (VA quanto por via óssea (VO. Para cada frequência, foram avaliadas 12 orelhas em um total de 18 neonatos. A análise dos resultados avaliou o tempo de latência e a presença da onda V até a intensidade de 20 dBnNA para quatro intensidades e para os dois tipos de condução (aérea e óssea. Resultados observou-se aumento da latência da onda V com a diminuição da intensidade e maiores latências nas frequências mais baixas, tanto na VA quanto na VO. Porém, em fortes intensidades, em ambas condições, não houve diferença entre as latências de 0.5 e 1 kHz, contrariando os achados da literatura. Para VA, na frequência de 500 Hz, houve presença da onda V até 30 dBnNA em todas as orelhas, e em 1000 Hz, 11 orelhas (91,66 % apresentaram resposta em 20 dBnNA; nas demais frequências, 100% das orelhas avaliadas apresentaram resposta em 20 dBnNA. Na VO, a presença da onda V em 20 dBnNA foi observada em todas as frequências estudadas. Conclusão os valores descritos podem ser considerados normativos e utilizados na clinica como padrão de normalidade, auxiliando no diagnóstico diferencial da perda auditiva ao nascimento.

Validation of the dermal exposure model in ECETOC TRA.

Science.gov (United States)

Marquart, Hans; Franken, Remy; Goede, Henk; Fransman, Wouter; Schinkel, Jody

2017-08-01

The ECETOC TRA model (presently version 3.1) is often used to estimate worker inhalation and dermal exposure in regulatory risk assessment. The dermal model in ECETOC TRA has not yet been validated by comparison with independent measured exposure levels. This was the goal of the present study. Measured exposure levels and relevant contextual information were gathered via literature search, websites of relevant occupational health institutes and direct requests for data to industry. Exposure data were clustered in so-called exposure cases, which are sets of data from one data source that are expected to have the same values for input parameters in the ECETOC TRA dermal exposure model. For each exposure case, the 75th percentile of measured values was calculated, because the model intends to estimate these values. The input values for the parameters in ECETOC TRA were assigned by an expert elicitation and consensus building process, based on descriptions of relevant contextual information.From more than 35 data sources, 106 useful exposure cases were derived, that were used for direct comparison with the model estimates. The exposure cases covered a large part of the ECETOC TRA dermal exposure model. The model explained 37% of the variance in the 75th percentiles of measured values. In around 80% of the exposure cases, the model estimate was higher than the 75th percentile of measured values. In the remaining exposure cases, the model estimate may not be sufficiently conservative.The model was shown to have a clear bias towards (severe) overestimation of dermal exposure at low measured exposure values, while all cases of apparent underestimation by the ECETOC TRA dermal exposure model occurred at high measured exposure values. This can be partly explained by a built-in bias in the effect of concentration of substance in product used, duration of exposure and the use of protective gloves in the model. The effect of protective gloves was calculated to be on average a

Citoinclusão de capa leucocitária e medula óssea de cães: padronização da técnica

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M.B. Paiva

2014-04-01

Full Text Available A técnica de citoinclusão é amplamente utilizada e reconhecida por profissionais da área da saúde, em particular médicos patologistas, porém as informações sobre a aplicação desse método em medicina veterinária ainda são raras. Análises citológicas de medula óssea e da capa leucocitária (porção do sangue formada por concentrados de leucócitos são amplamente utilizadas no diagnóstico de patologias de origem neoplásica e não neoplásica e de agentes infecciosos. Embora a importância do uso da técnica seja amplamente reconhecida, não há registro da utilização de amostras citológicas de medula óssea e capa leucocitária na confecção de citoinclusões em blocos de parafina, como meio de diagnóstico no segmento médico veterinário. Assim, este trabalho tem como objetivo elaborar um protocolo de citoinclusão em parafina para amostras citológicas de medula óssea e capa leucocitária de cães. Foram submetidas à técnica de citoinclusão 110 amostras de capa leucocitária e 44 de medula óssea de cães portadores ou não de enfermidade sistêmica, sendo que em 68% destas, tanto para a capa leucocitária quanto para a medula óssea, as amostras se mostraram viáveis. A utilização do álcool 95% como fixador e de etapas no processamento histológico de 20 minutos (álcool absoluto, xilol e parafina, três banhos cada foi crucial para a qualidade dos cortes histológicos e para a análise microscópica dos espécimes corados pela hematoxilina-eosina. A separação mecânica da capa leucocitária e a centrifugação do aspirado de medula óssea foram eficientes e de baixo custo no preparo das citoinclusões. Ressalta-se a importância na padronização da técnica de citoinclusão, em particular para amostras de capa leucocitária e medula óssea, visando à obtenção de espécimes de qualidade independentemente das limitações de equipamentos.

I RAPPORTI TRA L'ALBANIA E LA GRAN BRETAGNA (1940-1950)

OpenAIRE

COTA, JULIAN

2012-01-01

La presente tesi ha avuto l’obiettivo di illustrare i rapporti tra l’Albania e la Gran Bretagna nel decennio tra il 1940 al 1950. Il lavoro viene svolto basandosi su tre momenti; a) l’ingresso dell’Albania nell’ottica della politica estera della Gran Bretagna e l’invio delle missioni militari britanniche in Albania. b) La fine della Guerra collocò l’Albania dall’altra parte della cortina di ferro rispetto ai britannici causando il peggioramento dei rapporti tra i due governi dopo gli...

Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells.

Science.gov (United States)

Wu, F; Hu, Y; Long, J; Zhou, Y J; Zhong, Y H; Liao, Z K; Liu, S Q; Zhou, F X; Zhou, Y F; Xie, C H

2009-02-01

TRAIL induces apoptosis in a variety of tumorigenic and transformed cell lines, but not in many normal cells. Recent studies have demonstrated that death receptor 5 (DR5), one of the two death receptors bound by TRAIL, showed expression in most malignantly transformed cells. This study evaluated effects of a monoclonal antibody (TRA-8) to human death receptor 5, combined with ionizing radiation, on radioresistant human larynx squamous carcinoma cell line (Hep-2R). Cells were treated with TRA-8 alone or in combination with radiation, cell viability inhibition was measured by MTT assay, and the induction of apoptosis was determined by Annexin V staining. Radionsensitivity of Hep-2R cells treated with TRA-8 were investigated with long-term clonogenic assays. Regulation of DR5 expression in cells after radiation was analyzed by indirect immunofluorescence using murine TRA-8 in combination with flow cytometry. The results suggested that TRA-8 enhanced radionsensitivity of Hep-2R cells, and that TRA-8 regulated Hep-2R cell cycle arrest at G2/M phase. Irradiation up-regulated the expression of DR5, and when combined with TRA-8 yielded optimal survival benefit. Therefore, TRA-8 can be used in combination with irradiation in radioresistant human larynx squamous carcinoma cells. Monoclonal antibodies such as TRA-8 may play an important role in the development of an effective treatment strategy for patients with radioresistant cancers.

Generation of Adducts of 4-Hydroxy-2-nonenal with Heat Shock 60 kDa Protein 1 in Human Promyelocytic HL-60 and Monocytic THP-1 Cell Lines

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Alessia Arcaro

2015-01-01

Full Text Available Heat shock 60 kDa protein 1 (HSP60 is a chaperone and stress response protein responsible for protein folding and delivery of endogenous peptides to antigen-presenting cells and also a target of autoimmunity implicated in the pathogenesis of atherosclerosis. By two-dimensional electrophoresis and mass spectrometry, we found that exposure of human promyelocytic HL-60 cells to a nontoxic concentration (10 μM of 4-hydroxy-2-nonenal (HNE yielded a HSP60 modified with HNE. We also detected adducts of HNE with putative uncharacterized protein CXorf49, the product of an open reading frame identified in various cell and tissue proteomes. Moreover, exposure of human monocytic THP-1 cells differentiated with phorbol 12-myristate 13-acetate to 10 μM HNE, and to light density lipoprotein modified with HNE (HNE-LDL or by copper-catalyzed oxidation (oxLDL, but not to native LDL, stimulated the formation of HNE adducts with HSP60, as detected by immunoprecipitation and western blot, well over basal levels. The identification of HNE-HSP60 adducts outlines a framework of mutually reinforcing interactions between endothelial cell stressors, like oxLDL and HSP60, whose possible outcomes, such as the amplification of endothelial dysfunction, the spreading of lipoxidative damage to other proteins, such as CXorf49, the activation of antigen-presenting cells, and the breaking of tolerance to HSP60 are discussed.

Breaking down pluripotency in the porcine embryo reveals both a premature and reticent stem cell state in the inner cell mass and unique expression profiles of the naive and primed stem cell states.

Science.gov (United States)

Hall, Vanessa Jane; Hyttel, Poul

2014-09-01

To date, it has been difficult to establish bona fide porcine embryonic stem cells (pESC) and stable induced pluripotent stem cells. Reasons for this remain unclear, but they may depend on inappropriate culture conditions. This study reports the most insights to date on genes expressed in the pluripotent cells of the porcine embryo, namely the inner cell mass (ICM), the trophectoderm-covered epiblast (EPI), and the embryonic disc epiblast (ED). Specifically, we reveal that the early porcine ICM represents a premature state of pluripotency due to lack of translation of key pluripotent proteins, and the late ICM enters a transient, reticent pluripotent state which lacks expression of most genes associated with pluripotency. We describe a unique expression profile of the porcine EPI, reflecting the naive stem cell state, including expression of OCT4, NANOG, CRIPTO, and SSEA-1; weak expression of NrOB1 and REX1; but very limited expression of genes in classical pathways involved in regulating pluripotency. The porcine ED, reflecting the primed stem cell state, can be characterized by the expression of OCT4, NANOG, SOX2, KLF4, cMYC, REX1, CRIPTO, and KLF2. Further cell culture experiments using inhibitors against FGF, JAK/STAT, BMP, WNT, and NODAL pathways on cell cultures derived from day 5 and 10 embryos reveal the importance of FGF, JAK/STAT, and BMP signaling in maintaining cell proliferation of pESCs in vitro. Together, this article provides new insights into the regulation of pluripotency, revealing unique stem cell states in the different porcine stem cell populations derived from the early developing embryo.

C{sub 60}{sup 3-} versus C{sub 60}{sup 4-} /C{sub 60}{sup 2-} - synthesis and characterization of five salts containing discrete fullerene anions

Energy Technology Data Exchange (ETDEWEB)

Boeddinghaus, M. Bele; Klein, Wilhelm; Wahl, Bernhard; Faessler, Thomas F. [Department of Chemistry, Technische Universitaet Muenchen, Garching (Germany); Jakes, Peter; Eichel, Ruediger A. [Institut fuer Energie- und Klimaforschung (IEK-9), Forschungszentrum Juelich (Germany)

2014-04-15

Five new compounds, [Rb(18crown-6)]{sub 3}[C{sub 60}] (1), [Rb(18crown-6)]{sub 6}[C{sub 60}]{sub 2}(C{sub 3}H{sub 7}NO){sub 2}(C{sub 4}H{sub 8}O){sub 2} (2), [Rb(benzo18crown-6)]{sub 6}[C{sub 60}]{sub 2}(C{sub 2}H{sub 8}N{sub 2}){sub 5} (3), [Cs(benzo18crown-6)]{sub 3}C{sub 60}(C{sub 2}H{sub 8}N{sub 2}){sub 2} (4), and [Cs{sub 3}(benzo18crown-6){sub 5}]C{sub 60}(C{sub 2}H{sub 8}N{sub 2}){sub (4.5+x)} (5) were synthesized and characterized by single-crystal X-ray structure determination. All compounds contain discrete C{sub 60} anions, which are ordered in 1, 2, and 4, where direct cation-anion contacts occur. The unit cells of 1 and 2 contain two independent fullerides, which coordinate to the rubidium atoms either of two or of four [Rb(18crown-6)] units. Owing to the presence of differently coordinated fullerene units in compounds 1 and 2, a possible disproportionation of C{sub 60}{sup 3-} into C{sub 60}{sup 2-} and C{sub 60}{sup 4-} anions is discussed. In 3 and 4 the C{sub 60} anions are coordinated by three Rb and Cs atoms, respectively. In all compounds the average charge of the anion is -3. Magnetic data reveal a doublet spin state for 3. The EPR spectra are discussed for compounds 3 and 5. The role of a dynamic Jahn-Teller distortion is discussed, and we report the first IR spectroscopic data of fullerene trianions, which have been obtained in solution. (Copyright copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities

International Nuclear Information System (INIS)

Go, Masahiro J.; Takenaka, Chiemi; Ohgushi, Hajime

2008-01-01

Mesenchymal stem cells (MSCs) derived from human bone marrow have capability to differentiate into cells of mesenchymal lineage. The cells have already been applied in various clinical situations because of their expansion and differentiation capabilities. The cells lose their capabilities after several passages, however. With the aim of conferring higher capability on human bone marrow MSCs, we introduced the Sox2 or Nanog gene into the cells. Sox2 and Nanog are not only essential for pluripotency and self-renewal of embryonic stem cells, but also expressed in somatic stem cells that have superior expansion and differentiation potentials. We found that Sox2-expressing MSCs showed consistent proliferation and osteogenic capability in culture media containing basic fibroblast growth factor (bFGF) compared to control cells. Significantly, in the presence of bFGF in culture media, most of the Sox2-expressing cells were small, whereas the control cells were elongated in shape. We also found that Nanog-expressing cells even in the absence of bFGF had much higher capabilities for expansion and osteogenesis than control cells. These results demonstrate not only an effective way to maintain proliferation and differentiation potentials of MSCs but also an important implication about the function of bFGF for self-renewal of stem cells including MSCs

Stresses in implant-supported overdentures with bone resorption: a 3-D finite element analysis = Tensões em sobredentaduras com reabsorção óssea: análise por elementos finitos tridimensional

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Mariano, Luiz Oscar Honorato

2012-01-01

Conclusão: Os resultados sugerem que a perda óssea periimplantar aumenta a concentração de tensões nos implantes, pilares e osso marginal independentemente da reabsorção óssea na crista distal

Testes de função pulmonar no transplante de medula óssea: Revisão sistemática

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Eliane Viana Mancuso

2006-01-01

Full Text Available Resumo: As complicações pulmonares constituem causa importante de morbidade e mortalidade em doentes submetidos a transplante de medula óssea. Os testes de função pulmonar são utilizados rotineiramente na avaliação antes e no acompanhamento após o transplante. A revisão sistemática da literatura mostrou que a presença de alterações nos testes de função pulmonar antes do transplante de medula não esteve relacionada com maior incidência de complicações pulmonares pós-transplante. Entretanto, alterações destes testes após o transplante estiveram relacionadas com maior incidência de complicações respiratórias. Desta forma, embora as alterações dos testes de função pulmonar pré-transplante não tenham sido de valor preditivo positivo na detecção precoce de complicações respiratórias pós-transplante, os mesmos podem ser úteis na comparação com os testes realizados após o transplante e devem fazer parte da avaliação de doentes candidatos ao transplante de medula óssea.Rev Port Pneumol 2006; XII (1: 61-69 Abstract: The pulmonary function test plays an important role in the management of pulmonary complications after bone marrow transplantation. Although its utility in helping to predict the likelihood of developing post transplant pulmonary complications and mortality is not well established, current data indicate that pre-transplant pulmonary function tests are important as a reference for the interpretation of post transplant pulmonary function tests and for identifying patients at high risk of developing pulmonary complications and/or mortality after bone marrow transplantation.Rev Port Pneumol 2006; XII (1: 61-69 Palavras-chave: Transplante de medula óssea, testes de função pulmonar, revisão sistemática, Key-words: Bone marrow transplantation, respiratory function tests, systematic review

DENSITOMETRIA ÓSSEA, CTX, NÍVEL DE ATIVIDADE FÍSICA E FORÇA NA PÓS-MENOPAUSA

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Cristiane Fialho Ferreira da Silva

Full Text Available RESUMO Introdução: O tecido ósseo pode ser avaliado quantitativamente pela densitometria, porém ao associar os biomarcadores ósseos é possível predizer fragilidade óssea mais precocemente. Contudo, pouco se sabe sobre correlação entre ambos, como também sobre o nível de atividade física atual e a força muscular. Objetivo: Avaliar a correlação dos valores da densidade mineral óssea (DMO aos do biomarcador sanguíneo ósseo (CTX e também com o nível de atividade física mensurada pelo pedômetro e o questionário IPAQ, versão longa e com os testes de força em mulheres na pós-menopausa. Método: Estudo transversal e descritivo de 62 mulheres na pós-menopausa, saudáveis, média de idade de 56,82 ± 4,02 anos, avaliadas quanto ao nível de atividade física atual, força, DMO e CTX. Foi realizada densitometria óssea corporal total, de coluna lombar, fêmur e antebraços, além de mensuração de CTX, cálcio iônico, fósforo e PTH. Dois grupos foram formados: controle com DMO normal e grupo com DMO diminuída. Utilizou-se teste t de Student, qui-quadrado e Pearson. Resultados: Não houve diferenças estatisticamente significativas entre os dois grupos para testes de força, nível de atividade física atual e força muscular com relação ao CTX, porém houve associação do valor deste à DMO. Conclusão: Foi demonstrada dependência do CTX com relação aos valores de DMO total e da coluna lombar, ocorrendo baixa DMO quando o CTX estava entre moderado e alto. Isso indica que esse biomarcador talvez possa ser utilizado como forma de triagem de indivíduos com risco de baixa DMO e maior risco de fraturas.

Prevalência de doença mineral óssea em adolescentes com fibrose cística Prevalence of bone mineral disease among adolescents with cystic fibrosis

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Reinaldo José do Amaral Caldeira

2008-02-01

Full Text Available OBJETIVO: Avaliar a prevalência de doença mineral óssea em adolescentes com fibrose cística e associar os achados com as variáveis estudadas. MÉTODOS: Foram selecionados 37 adolescentes, dos quais foram avaliados: estado nutricional pelos índices de altura/idade e massa corporal/idade; densidade mineral óssea da coluna lombar e corpo inteiro por densitometria com emissão de raio X de dupla energia; ingestão dietética diária pelo registro alimentar de 3 dias; e prova de função pulmonar pelo volume expiratório forçado no primeiro segundo. RESULTADOS: A média de idade foi de 13,2 (±2,8 anos. O estado nutricional adequado foi de 70,3 e 75,7% pelos índices de altura/idade e de massa corporal/idade, respectivamente; 54,1% dos pacientes apresentaram redução da densidade mineral óssea para coluna lombar e 32,5% para corpo inteiro. Houve correlação positiva entre densidade mineral óssea e índice de massa corporal (p = 0,04. A doença pulmonar e a insuficiência pancreática apresentaram correlação com a alteração da densidade mineral óssea. O inquérito alimentar revelou percentuais de adequação para o cálcio, fósforo e calorias, de acordo com a recomendação nutricional preconizada pelo Consenso Europeu de Fibrose Cística. Essas variáveis não se mostraram estatisticamente significantes na análise multivariada. CONCLUSÃO: A prevalência de doença mineral óssea é alta na adolescência. O estado nutricional adequado, a reposição de enzimas pancreáticas e o controle da doença pulmonar podem ter efeito protetor para a massa óssea.OBJECTIVE: To evaluate the prevalence of bone mineral disease among adolescents with cystic fibrosis and to relate the findings with the variables studied. METHODS: The study enrolled 37 adolescents who were assessed for: nutritional status according to height/age and body mass/age ratios; bone mineral density of the lumbar spine and entire body by densitometry with dual emission X

ICT: interfacce tra persone e luoghi

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Corinna Morandi

2013-04-01

Full Text Available Nonostante la diffusione del paradigma della smart city sia stata esponenziale, con l’effetto di confonderne il significato, il concetto di “città intelligente” indica comunque un tema di ricerca importante, in particolare quando venga esteso a contesti territoriali più ampi. In questo senso, la ricerca La smart region tra Torino e Milano. I servizi mobili come driver di innovazione territoriale in vista di Expo 2015 (Politecnico di Milano-Telecom Italia si propone di sperimentare l’uso delle ICT per un sistema integrato di servizi nel territorio tra Torino e Milano, anche in prospettiva di Expo 2015: una regione metropolitana valorizzata da un sistema di relazioni già attive e inserita nel contesto più ampio della city-region del Nord Italia, il cui sviluppo va legato ad una strategia di scala vasta, declinabile nella definizione di “smart city-region” o, più semplicemente, di “smart region”. In tale contesto, il ruolo delle università nei processi di sviluppo territoriale, il miglioramento dei servizi materiali e immateriali erogati dai campus, su cui la ricerca (in corso ha scelto di concentrarsi, può contribuire ad aumentare la sostenibilità e competitività del territorio. Una prima sperimentazione è stata condotta sull’area di Città Studi a Milano, testando una metodologia utile a comprenderne le esigenze e individuando soluzioni e servizi funzionanti su  terminali di comunicazione mobile, interfaccia tra persone e luoghi nel campus e nel contesto urbano circostante.

Introducing the ARL X'Tra x-ray diffraction system

International Nuclear Information System (INIS)

Harris, L.

2002-01-01

Full text: The ARL X'Tra is a state-of-the-art solution for powder X-ray diffraction in a large range of applications such as pharmaceuticals and biosciences, chemicals, earth sciences, semi-conductors, metallurgy and ceramics. The X'Tra offers the latest technology in key diffraction components to produce a high performance instrument at an affordable price. This presentation examines some of the hardware and performance features of this instrument. Copyright (2002) Australian X-ray Analytical Association Inc

MSCs can be differentially isolated from maternal, middle and fetal segments of the human umbilical cord.

Science.gov (United States)

Lim, Jezamine; Razi, Zainul Rashid Mohamad; Law, Jiaxian; Nawi, Azmawati Mohammed; Idrus, Ruszymah Binti Haji; Ng, Min Hwei

2016-12-01

Human Wharton's jelly-derived mesenchymal stromal cells (hWJMSCs) are possibly the most suitable allogeneic cell source for stromal cell therapy and tissue engineering applications because of their hypo-immunogenic and non-tumorigenic properties, easy availability and minimal ethical concerns. Furthermore, hWJMSCs possess unique properties of both adult mesenchymal stromal cells and embryonic stromal cells. The human umbilical cord (UC) is approximately 50-60 cm long and the existing studies in the literature have not provided information on which segment of the UC was studied. In this study, hWJMSCs derived from three anatomical segments of the UC are compared. Three segments of the whole UC, each 3 cm in length, were identified anatomically as the maternal, middle and fetal segments. The hWJMSCs from the different segments were analyzed via trypan blue exclusion assay to determine the growth kinetics and cell viability, flow cytometry for immunophenotyping and immunofluorescence and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of stromal cell transcriptional factors. Furthermore, the trilineage differentiation potential (osteogenic, adipogenic and chondrogenic) of these cells was also assessed. hWJMSCs isolated from the maternal and fetal segments displayed greater viability and possessed a significantly higher proliferation rate compared with cells from the middle segment. Immunophenotyping revealed that hWJMSCs derived from all three segments expressed the MSC markers CD105, CD73, CD90, CD44, CD13 and CD29, as well as HLA-ABC and HLA-DR, but were negative for hematopoietic markers CD14, CD34 and CD45. Analysis of the embryonic markers showed that all three segments expressed Nanog and Oct 3/4, but only the maternal and fetal segments expressed SSEA 4 and TRA-160. Cells from all three segments were able to differentiate into chondrogenic, osteogenic and adipogenic lineages with the middle segments showing much lower differentiation

Effect of combined treatment with calcitonin on bone densitometry of patients with treated hypothyroidism Influência da terapêutica associada com calcitonina sobre a densitometria óssea de pacientes com hipotiroidismo tratado

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F. J. da C. Stamato

2000-06-01

Full Text Available INTRODUCTION: Thyroid hormones (TH may affect bone metabolism and turnover, inducing a loss of bone mass among hyperthyroid and in hypothyroid patients under hormone replacement treatment. Thyroid dysfunction leads to changes in the dynamics of parathyroid hormone (PTH and calcitonin (CT secretion. OBJECTIVE: The objective of the study was to determine the usefulness of CT as adjuvant therapy in the prevention of bone loss during the treatment of hypothyroidism. MATERIAL AND METHODS: We studied 16 female patients with recently diagnosed primary hypothyroidism, divided into two groups: group G1 (n=8 submitted to treatment with thyroxine (L-T4, and Group 2 (n=8 that, in addition to being treated with L-T4, received a nasal CT spray. All patients were submitted to determination of TSH, free T4, bone mineral densitometry (BMD and total bone calcium (TBC at the time of diagnosis, after 6 to 9 months of treatment, and after 12 months of treatment. RESULTS: No statistical significant differences were detected in either group between the total BMD values obtained for the femur and lumbar spine before and after treatment. However, group G1 presented a statistical significant TBC loss after 12 months of treatment compared to initial values. In contrast, no TBC loss was observed in the group treated with LT-4 in combination with CT, a fact that may suggest that CT was responsible for the lower bone reabsorption during treatment of hypothyroidism.INTRODUÇÃO: Os hormônios tiroidianos (HT podem influenciar o metabolismo e o "turnover" ósseo, induzindo perda de massa óssea em hipertiróideos e em hipótiroideos na vigência de reposição hormonal. As disfunções tiroidianas levam a alterações na dinâmica de secreção de paratormônio (PTH e de calcitonina (CT. OBJETIVO: Esclarecer a utilidade da CT como terapêutica coadjuvante na prevenção de perda óssea durante o tratamento do hipotiroidismo. MATERIAL E MÉTODOS: Dezeseis pacientes do sexo

Implante de biomateriais e a consolidação óssea em cadelas submetidas à ovariossalpingo-histerectomia Influence of biomaterials on the bony consolidation in spayed female dogs

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A.I. Roque-Rodriguez

2009-10-01

Full Text Available Avaliou-se a hidroxiapatita com alandronato e hidroxiapatita com colágeno na aceleração da consolidação óssea do rádio de cadelas adultas submetidas à ovariossalpingo-histerectomia (OSH. Utilizaram-se 14 cadelas adultas, distribuídas aleatoriamente em dois grupos: grupo-controle e grupo OSH (submetidas à OSH. Quatro meses após a OSH, as cadelas dos dois grupos foram submetidas à cirurgia para produção de uma falha óssea de 4mm de diâmetro nos terços distal e proximal do rádio. No terço distal do membro direito, foi utilizada a hidroxiapatita com alandronato e, no membro esquerdo, a hidroxiapatita com colágeno; no terço proximal, não se utilizou nenhum biomaterial. Houve retardo na consolidação das falhas ósseas nas cadelas submetidas à OSH comparadas com as não submetidas. A hidroxiapatita com alandronato acelerou o processo de reparação e, em todos os animais dos dois grupos, a densidade óssea foi significativamente maior no terço distal onde foi implantada. Os dois biomateriais apresentaram biocompatibilidade, constatada pela ausência de reação inflamatória ou outra reação indesejável.The hydroxyapatite with alendronate and hydroxyapatite with collagen were evaluated in the acceleration of the bony consolidation of adult spayed bitch radius. For that, 14 adult bitches were distributed in two groups (control and spayed. Four months after ovariohysterectomy, the groups were submitted to the surgery for production of a 4mm diameter bony flaw in the distal and proximal third regions of the radius. In the distal region of the right thoracic limb, hydroxyapatite with alendronate was used. In the distal region of the left thoracic limb, hydroxyapatite with collagen was used. Any biomaterial was used in proximal part of the limb. There was a retard in bony flaws consolidation in the spayed bitches. Hydroxyapatite with alendronate showed better result, since the place it was implanted considerably increased the

Metaplasia óssea e esterilidade primária

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Reggiani Celeste P. Demeterco

2001-01-01

Full Text Available A metaplasia óssea do endométrio é uma condição rara e pode ser explicada por uma neoformação no endométrio, com deposição de sais de cálcio. Entretanto, a situação mais comum é a história prévia de abortamento com permanência de ossículos na cavidade uterina. Foi relatado um caso de ossificação endometrial em uma mulher de 31 anos, sem gravidez prévia ou história de aborto, apresentando dismenorréia e infertilidade. A ultra-sonografia pélvica mostrou área hiperecóica no fundo da cavidade uterina. A histeroscopia foi realizada e uma imagem sugestiva de tecido ósseo foi encontrada. Este tecido foi retirado e a histopatologia confirmou a ossificação endometrial.

Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity.

Science.gov (United States)

Finley, Lydia W S; Vardhana, Santosha A; Carey, Bryce W; Alonso-Curbelo, Direna; Koche, Richard; Chen, Yanyang; Wen, Duancheng; King, Bryan; Radler, Megan R; Rafii, Shahin; Lowe, Scott W; Allis, C David; Thompson, Craig B

2018-05-01

A robust network of transcription factors and an open chromatin landscape are hallmarks of the naive pluripotent state. Recently, the acetyllysine reader Brd4 has been implicated in stem cell maintenance, but the relative contribution of Brd4 to pluripotency remains unclear. Here, we show that Brd4 is dispensable for self-renewal and pluripotency of embryonic stem cells (ESCs). When maintained in their ground state, ESCs retain transcription factor binding and chromatin accessibility independent of Brd4 function or expression. In metastable ESCs, Brd4 independence can be achieved by increased expression of pluripotency transcription factors, including STAT3, Nanog or Klf4, so long as the DNA methylcytosine oxidases Tet1 and Tet2 are present. These data reveal that Brd4 is not essential for ESC self-renewal. Rather, the levels of pluripotency transcription factor abundance and Tet1/2 function determine the extent to which bromodomain recognition of protein acetylation contributes to the maintenance of gene expression and cell identity.

A photometric analysis of the neglected EW-type binary V336 TrA

Science.gov (United States)

Kriwattanawong, W.; Sarotsakulchai, T.; Maungkorn, S.; Reichart, D. E.; Haislip, J. B.; Kouprianov, V. V.; LaCluyze, A. P.; Moore, J. P.

2018-05-01

This study presents an analysis of photometric light curves and absolute parameters for the EW-type binary V336 TrA. VRI imaging observations were taken in 2013 by using the robotic telescopes PROMPT 4 and PROMPT 5 at Cerro Tololo Inter-American Observatory (CTIO), Chile. The observed light curves were fitted by using the Wilson-Devinney method. The results showed that V336 TrA is a W-type contact binary with a mass ratio of q = 1.396. The binary is a weak contact system with a fill-out factor of f = 15.69%. The system contains components with masses of 0.653 M⊙ and 0.912 M⊙ for the hotter and the cooler, respectively. The location of the secondary (less massive) component on the log M - log L diagram was found to be near the TAMS. The component has evolved to be oversize and overluminous. The orbital angular momentum of the binary was found to be log Jo = 51.61 cgs, less than all detached systems for same mass. The system has undergone angular momentum and/or mass loss, during the binary evolution from the detached to contact system.

Giocare, tra violenza ed ecologia della mente.

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Enrico Euli

2008-07-01

Full Text Available L'articolo riflette sulle relazioni tra comunicazione, violenza e gioco, nel tentativo di rintracciare tra loro convergenze, affinità e contrasti. Le nostre relazioni, infatti, ci immettono inerzialmente in dilemmi e paradossi di così alta complessità da rendere spesso inadeguati gli strumenti 'logici' ed 'analitici': dal che può derivare un senso di impotenza, rabbia, paralisi, ma anche un desiderio potente di controllo e di soluzione degli eventi. Entrambe queste strade ci conducono dentro le false alternative della violenza. La cultura del gioco, invece, ci propone (attraverso le sue pratiche, ma anche negli scritti teorici di Freud, Winnicott, Bateson, qui riassunti e rivisitati una modalità più creativa e nonviolenta, più prossima ad un' ecologia della mente e ad un'estetica delle emozioni.

Equação preditora de idade óssea na iniciação esportiva através de variáveis antropométricas

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Breno Guilherme de Araújo T. Cabral

2013-04-01

Full Text Available INTRODUÇÃO: Atualmente o desempenho esportivo tem se desenvolvido de forma rápida e concreta, exigindo, dessa forma, uma eficiente evolução das diferentes áreas das ciências do esporte, oferecendo o suporte cientifico necessário à continuidade desse fenômeno. OBJETIVO: O estudo em questão teve como objetivo desenvolver uma equação matemática preditora de idade óssea de jovens atletas, com base em variáveis antropométricas. MÉTODOS: Para se alcançar o resultado final foram medidas dobras cutâneas, perímetros corporais e diâmetros ósseos, idade cronológica e idade óssea. O tratamento estatístico utilizou a correlação entre as variáveis, a regressão linear múltipla com modelagem backward. RESULTADO: Se obteve como resultado final um modelo de equação que explica 75,4% da variação da idade óssea com r = 0,868 e R² = 0,754, utilizando idade cronológica, estatura, dobra tricipital, perímetro corrigido de braço e diâmetros de fêmur e úmero. CONCLUSÃO: Concluiu-se a partir dos resultados, que esse tipo de modelo revela valores aproximados aos observados através da radiografia de mão e punho, tornando-se importante na observação do estágio maturacional através de testes de fácil aplicabilidade por profissionais da área esportiva.

Padrão lesional da medula óssea na imunodeficiência adquirida (AIDS

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M. Barretto Netto

1988-08-01

Full Text Available O estudo da medula óssea em 20 casos de necrópsia de pacientes portadores da Síndrome da Imunodeficiência Adquirida sugere que as alterações medulares são freqüentes e de valor diagnóstico no reconhecimento da Síndrome. É admitida a existência de um padrão lesional diagnóstico onde predominam os seguintes elementos: hipercelularidade, ausência ou redução da granulopoiese, plasmocitose, histiocitose e displasia megacariocítica.

Avaliação de centrifugado osteogênico de medula óssea na consolidação de fratura em coelhos Evaluation of centrifuged osteogenic bone marrow in fracture consolidation in rabbits

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Carlos Eduardo Sanches Vaz

2010-01-01

Full Text Available OBJETIVO: Avaliar a eficácia de um centrifugado osteogênico de medula óssea em estimular a consolidação de osteotomias da fíbula em coelhos. MÉTODOS: Foram utilizados dez coelhos machos adultos da raça Nova Zelândia albino. Realizou-se uma osteotomia transversa médio-diafisária da fíbula direita, seguida da adição local de uma esponja de colágeno absorvível embebida em um centrifugado osteogênico, obtido pela centrifugação de aspirado de medula óssea do osso ilíaco ipsilateral. A fíbula esquerda foi utilizada como controle, sendo feita a mesma osteotomia, porém neste caso adicionando-se somente a esponja de colágeno absorvível. Após quatro semanas os animais foram sacrificados para estudo dos calos ósseos formados. Os critérios de avaliação foram a mensuração da densidade mineral utilizando-se a densitometria óssea com DEXA, do volume do calo com tomografia computadorizada multi-slice e dos tecidos formados por meio de histomorfometria. RESULTADOS: A utilização do centrifugado osteogênico resultou em um aumento médio na densidade mineral óssea dos calos de 40,3% e da quantidade relativa de tecido ósseo de 9,4%, sem aumento significativo nas quantidades relativas de cartilagem ou fibrose nem no volume do calo ósseo. CONCLUSÃO: A administração do centrifugado osteogênico de medula óssea utilizado neste estudo favoreceu a consolidação óssea de osteotomias experimentais em coelhos.OBJECTIVE: The purpose of this study was to evaluate the efficacy of a centrifuged osteogenic bone marrow aspirate to stimulate healing in rabbit fibular osteotomies. METHODS: Ten white New Zealand rabbits were used. A transverse medial diaphyseal fibular osteotomy was performed on the right fibula, where an absorbable collagen sponge embedded in osteogenic centrifuged bone marrow aspirate, obtained from the ipsilateral iliac bone, was inserted. The left fibula was used as the control group, where the collagen absorbable

Lesao óssea em leucemias agudas linfoblásticas tipo T e TIPO nao T / nao B

OpenAIRE

Martins, Fernando Lopes

2013-01-01

Resumo: O AUTOR APRESENTA OS RESULTADOS DO ESTUDO RADIOLÓGICO DE ESQUELETO DE 61 PACIENTES PORTADORES DE LEUCEMIA AGUDA LINFOBLÁSTICA, CORRELACIONANDO A PRESENÇA OU NÃO DE Lesões ÓSSEAS COM A CLASSIFICAÇÃO IMUNOLÓGICA DE LAL TIPO T E TIPO não T / não B

A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

DEFF Research Database (Denmark)

Zou, Lijin; Luo, Yonglun; Chen, Muwan

2013-01-01

We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri......-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer......, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future....

Estudo comparativo da neoformação óssea utilizando-se o enxerto autógeno e três substitutos: defeitos ósseos em ratos Comparative study of bone neoformation using autologous grafting and three replacements: bone defects in rats

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Rodrigo Steffen Stein

2009-01-01

Full Text Available OBJETIVO: Comparar a percentagem de neoformação óssea promovida pelo enxerto ósseo autógeno e três tipos de materiais de substituição de características distintas em cavidades em fêmures de ratos. MÉTODOS: Foram realizadas duas cavidades de 5,4 x 2,7mm, em cada fêmur (direito e esquerdo, de 14 ratos Wistar isogênicos. Cada um dos quatro defeitos criados foi preenchido com o osso autógeno ou com um dos três materiais testados - hidroxiapatita (HA, Genphos® (HA+ β-TCP e GenMix® (um enxerto ósseo bovino composto. Ao final dos períodos de seis semanas (n = 6 e 12 semanas (n = 8, os animais foram sacrificados. As lâminas (coradas com Picro-Sirius foram analisadas por microscopia óptica normal e software específico. RESULTADOS: Os grupos com o osso autógeno se mostraram muito superiores aos demais nos dois tempos analisados, tendo média de neoformação óssea ± DP de 90,6 ± 10,8% em seis semanas, e 98 ± 9,2% em 12 semanas (p > 0,0001 em ambos os tempos analisados. Em seis semanas, os resultados para os demais grupos foram os seguintes: Genphos®, 46 ± 7,1%; HA, 43,1 ± 8,4%; e GenMix®, 57,3 ± 4,5%. Em 12 semanas: Genphos®, 47,8 ± 11,1%; HA, 39,9 ± 5,4%; GenMix®, 59,7 ± 4,8%, significativa (p = 0,007. CONCLUSÕES: Em ambos os tempos analisados, os três materiais de substituição óssea testados se mostraram inferiores ao osso autógeno quanto à percentagem de neoformação óssea.OBJECTIVE: Compare the percentage of bone neoformation promoted by autologous bone grafting and three kinds of replacement materials with different characteristics in rats' femoral holes. METHODS: Two holes measuring 5.4 x 2.7mm, were produced on each femur (right and left of 14 isogenic Wistar rats. Each of the four defects produced was filled by autologous bone or by one of three tested materials - hydroxyapatite (HA, Genphos® (HA+ β-TCP and GenMix® (a combined bovine bone graft. In the end of the 6-week (n = 6 and 12-week (n = 8

Estudo retrospectivo do tratamento de leucemia mielóide aguda com o transplante de medula óssea: a experiência brasileira Retrospective study of stem cell transplantation for acute myeloid leukemia (AML: the Brazilian experience

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Nelson Hamerschlak

2006-03-01

Full Text Available Dados do Registro Internacional de Transplante de Medula Óssea, International Bone Marrow Transplant Registry (IBMTR contribuem para o progresso do transplante de medula óssea (TMO em todo o mundo. Neste artigo relatamos a experiência brasileira em leucemia mielóide aguda e comparamos os resultados do TMO com os dados internacionais. Foi realizado um estudo retrospectivo com dados de tratamento de LMA com o TMO de 16 instituições brasileiras. A análise estatística dos transplantes da modalidade autogênica (TMO auto e alogênica (TMO alo foi realizada com o método de Kaplan-Meier e log-rank. Todos os valores de p foram bicaudados. Foram avaliados os dados de 731 pacientes (205 TMO auto e 526 TMO alo. A mediana de sobrevida global dos pacientes submetidos ao TMO auto foi superior à dos submetidos ao TMO alo (1.035 vs 466 dias, p=0,0012. A origem das células-tronco (OCT no TMO alo em 73% dos pacientes foi de medula óssea (CTMO, em 23% de sangue periférico (CTSP e em 4% de cordão umbilical. No TMO auto, a OCT foi 63% de CTSP, 22% CTMO e 15% de ambas as fontes. A OCT não teve impacto na sobrevida global (SG. Não houve diferença na SG também entre os pacientes segundo a classificação FAB no TMO alo, mas os pacientes com LMA M3 com o TMO auto tiveram SG longa. Como esperado, a principal causa de óbito entre os pacientes do TMO auto foi relacionada à recidiva de doença (60%, enquanto no TMO alo as principais causas foram a doença enxerto versus hospedeiro e infecções (38%. Em ambos os grupos foi observada SG mais longa nos pacientes tratados em primeira remissão completa (1RC quando comparados aos de segunda remissão (2RC e outras fases (pData from the International Bone Marrow Transplant Registry (IBMTR contribute for the improvement of Bone Marrow Transplant (BMT worldwide. We studied the Brazilian experience in BMT for AML to compare this with international data. We performed a retrospective study by sending

Final Report of the NASA Technology Readiness Assessment (TRA) Study Team

Science.gov (United States)

Hirshorn, Steven; Jefferies, Sharon

2016-01-01

The material in this report covers the results on the NASA-wide TRA team, who are responsible for ascertaining the full extent of issues and ambiguities pertaining to TRATRL and to provide recommendations for mitigation. The team worked for approximately 6 months to become knowledgeable on the current TRATRL process and guidance and to derive recommendations for improvement.The team reviewed the TRA processes of other government agencies (OGA), including international agencies, and found that while the high-level processes are similar, the NASA process has a greater level of detail. Finally, NASA’s HQ OCT continues to monitor the GAO’s efforts to produce a TRA Best Practices Guide, a draft of which was received in February 2016. This Guide could impact the recommendations of this report.

Regeneração Óssea Guiada Em Região Estética - Revisão De Literatura

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Alvin Tomm

2017-12-01

Full Text Available Introdução: a Regeneração Óssea Guiada tem por objetivo a reconstrução do rebordo alveolar utilizando membranas. Este procedimento é indicado quando não existe tecido ósseo suficiente para a colocação de implantes ou nos casos em que a estética é necessária. Objetivo: verificar, por meio de revisão de literatura, a previsibilidade da Regeneração Óssea Guiada em região estética. Métodos: a presente revisão de literatura contou com a pesquisa em artigos cientificos, indexados na base de dados Pubmed, buscando as seguintes palavras-chave: Esthetics, Guided bone regeneration (GBR, Dental Implant, Long-term, Follow-up. Conclusão: a literatura mostra que a técnica de regeneração é viável e apresenta longevidade ao longo do tempo.

Avaliação da fosfatase alcalina óssea e hepática em gatos com tirotoxicose induzida

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Fabiano Séllos Costa

2009-10-01

Full Text Available A tirotoxicose é caracterizada pelas excessivas concentrações séricas dos hormônios tiroidianos, podendo desencadear graves alterações no metabolismo ósseo, sendo a elevação da fosfatase alcalina total uma alteração laboratorial freqüentemente observada no hipertiroidismo felino. O aumento global dos níveis séricos de fosfatase alcalina pode ser decorrente de diferentes isoenzimas e, no caso do hipertiroidismo em humanos, as isoenzimas de origem óssea e hepática apresentam-se comumente elevadas. A partir da avaliação da bioquímica sérica de oito gatos com tirotoxicose induzida e elevação da fosfatase alcalina associada, o presente trabalho demonstra um aumento significativamente maior dos níveis séricos da fosfatase alcalina de origem óssea quando comparado com a isoenzima de origem hepática. Conclui-se que as alterações no metabolismo ósseo foram as principais responsáveis pelo aumento da fosfatase alcalina nos gatos com tirotoxicose induzida.

AcEST: BP915959 [AcEST

Lifescience Database Archive (English)

Full Text Available RNA synthetase OS=Desulfovibrio v... 60 4e-08 tr|A6GTU1|A6GTU1_9BURK Leucyl-tRNA synthetase OS=Limnobacter s...27 >tr|A6GTU1|A6GTU1_9BURK Leucyl-tRNA synthetase OS=Limnobacter sp. MED105 GN=LMED105_15234 PE=3 SV=1 Lengt

“Aspectos histológicos e imuno-histoquímicos das biópsias de medula óssea avaliadas no período de 2011 a 2013 em dois laboratórios gerais de anatomia patológica: dificuldades, facilidades e conclusões”

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Maria Cecília Ferro

2015-10-01

Full Text Available A biópsia de medula óssea (BMO é parte integrante do estadiamento e seguimento de pacientes hematológicos e, a sua avaliação oferece uma análise precisa da celularidade (relação tecido adiposo/tecido hematopoético, organização topográfica dos diferentes elementos celulares da medula óssea, alterações do estroma (fibrose reticulínica e colagênica, alterações morfológicas/topográficas dos elementos maduros da linhagem megacariocítica e lesões focais (metástases, infiltração por Linfoma Hodgkin e Linfoma não Hodgkin, granulomas.1 Acrescido a este estudo anatomopatológico, a imunohistoquímica, com marcadores celulares na biópsia de medula óssea, fixada em parafina, representa uma poderosa ferramenta de diagnóstico, de forma que seus resultados podem ser facilmente correlacionados com os obtidos por outras técnicas como a citometria de fluxo e, acima de tudo, os achados clínicos.2 No entanto, apesar de todo o avanço tecnológico nos instrumentos usados no estudo anatomopatológico, mesmo os hematopatologistas treinados precisam de informações clínicas hematológicas precisas, oportunidades de inter-relação com os hematologistas e na maioria dos casos de estudo imuno-histoquímico do material em questão.3 O presente trabalho tem como objetivo analisar os resultados dos casos de biópsia de MO nos últimos três anos, comparar os resultados da imuno-histoquímica com os dados da identificação (idade, gênero e das informações clínicas e hematológicas do paciente. Foi feito um levantamento retrospectivo das biópsias de medula óssea em dois laboratórios do município de Sorocaba (Laboratório de Patologia da Faculdade de Ciências Médicas e da Saúde de Sorocaba – FCMS/PUC-SP, e Laboratório de Patologia e Citologia de Sorocaba. Foram analisados 190 resultados de exames anatomopatológicos de Medula Óssea, sendo 51,6% (n=98 de pacientes do sexo feminino e 48,4% (n=92 do sexo masculino. Apenas 141

Characterization of Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma

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Helen H Yu

2016-08-01

Full Text Available Aim To identify and characterize cancer stem cells (CSC in moderately differentiated buccal mucosa squamous cell carcinoma (MDBMSCC. Methods 4μm-thick formalin-fixed paraffin-embedded MDBMSCC samples from six patients underwent 3,3-diaminobenzidine (DAB immunohistochemical (IHC staining for the embryonic stem cell (ESC markers NANOG, OCT4, SALL4, SOX2 and pSTAT3; cancer stem cell marker CD44; squamous cell carcinoma (SCC marker EMA; and endothelial marker CD34. The transcriptional activities of the genes encoding NANOG, OCT4, SOX2, SALL4, STAT3 and CD44 were studied using NanoString gene expression analysis and colorimetric in situ hybridization (CISH for NANOG, OCT4, SOX2, SALL4 and STAT3. Results DAB and immunofluorescent (IF IHC staining demonstrated the presence of (1 an EMA+/CD44+/SOX2+/SALL4+/OCT4+/pSTAT3+/NANOG+ CSC subpopulation within the tumor nests; (2 an EMA-/CD44-/CD34-/SOX2+/OCT4+/pSTAT3+/NANOG+ subpopulation within the stroma between the tumor nests; and (3 an EMA-/CD44-/CD34+/SOX2+/ SALL4+/OCT4+/pSTAT3+/NANOG+ subpopulation on the endothelium of the microvessels within the stroma. The expression of CD44, SOX2, SALL4, OCT4, pSTAT3 and NANOG was confirmed by the presence of mRNA transcripts, using NanoString analysis and NANOG, OCT4, SOX2, SALL4 and STAT3 by CISH staining. Conclusion This study demonstrated a novel finding of three separate CSC subpopulations within MDBMSCC: (1 within the tumor nests expressing EMA, CD44, SOX2, SALL4, OCT4, pSTAT3 and NANOG; (2 within the stroma expressing SOX2, SALL4, OCT4, pSTAT3 and NANOG; and (3 on the endothelium of the microvessels within the stroma expressing CD34, SOX2, SALL4, OCT4, pSTAT3 and NANOG.

Self-renewal and differentiation capabilities are variable between human embryonic stem cell lines I3, I6 and BG01V

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Rao Mahendra S

2009-06-01

Full Text Available Abstract Background A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs are not well known because of insufficient characterization. To address this question, a side-by-side comparison of 1 the ability to maintain an undifferentiated state and to self-renew under standard conditions; 2 the ability to spontaneously differentiate into three primary embryonic germ lineages in differentiating embryoid bodies; and 3 the responses to directed neural differentiation was made between three NIH registered hES cell lines I3 (TE03, I6 (TE06 and BG01V. Lines I3 and I6 possess normal XX and a normal XY karyotype while BG01V is a variant cell line with an abnormal karyotype derived from the karyotypically normal cell line BG01. Results Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. Undifferentiated I3 cells grew significantly slower but their differentiation potential was greater than I6 and BG01V. Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. Conclusion Our comparative analysis provides further evidence for similarities and differences between three h

Avaliação da densidade mineral óssea em pacientes com doença inflamatória intestinal Bone mineral density evaluation in inflammatory bowel disease patients

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Fabiana Lígia Lora

2005-12-01

Full Text Available RACIONAL: Pacientes com doença inflamatória intestinal têm maior prevalência de redução da densidade mineral óssea em comparação às pessoas saudáveis. OBJETIVO: Avaliar a densidade mineral óssea em uma população de pacientes com doença inflamatória intestinal. MÉTODOS: Noventa pacientes de 20 a 50 anos de idade, do ambulatório de doença inflamatória intestinal do Serviço de Gastroenterologia do Hospital de Clínicas da Universidade Federal do Paraná, Curitiba, PR, foram selecionados para avaliação. Desses, 76 completaram todas as etapas de avaliação. A densitometria foi realizada da coluna lombar e fêmur direito em aparelho Hologyc QDR 1000/W. RESULTADOS: Os pacientes com doença inflamatória intestinal tiveram significativa redução da densidade mineral óssea, avaliada por massa óssea em números absolutos (g/cm², em todas as regiões avaliadas, colo do fêmur, fêmur total e coluna lombar. As variáveis analisadas como, índice de atividade de doença, uso de corticóide, cirurgias prévias, índice de massa corpórea e falta de atividade física, não demonstraram correlação com a massa óssea, ou seja, não influenciaram os resultados da densidade mineral óssea no grupo estudado de doentes com doença inflamatória intestinal. CONCLUSÃO: Densidade mineral óssea reduzida foi encontrada nos pacientes com doença inflamatória intestinal do Ambulatório de Doença Inflamatória Intestinal do Serviço de Gastroenterologia do Hospital de Clínicas da Universidade Federal do Paraná, mais pronunciadamente nos pacientes com doença de Crohn, semelhante ao descrito na literatura. Nenhuma das variáveis analisadas mostrou correlação com a densidade mineral óssea.BACKGROUND: Inflammatory bowel disease patients have shown greater reduction of the bone mineral density compared to healthy people. AIM: To evaluate the bone mineral density in a population of patients with inflammatory bowel disease. METHODS: Ninety

Efeito da dexametasona e do cetoprofeno na osteogênese e na resistência óssea em ratos

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Patrícia Costa dos Santos da Silva

2012-04-01

Full Text Available O presente estudo teve como objetivo avaliar o efeito do cetoprofeno e da dexametasona na osteogênese ao redor de implante de hidroxiapatita densa (HAD na tíbia, no osso parietal, e na resistência óssea. Utilizaram-se 15 ratos Wistar, pesando 250±30 g, com 50 dias de idade. Após a anestesia com quetamina/xilazina IM, produziuse no osso parietal e na epífise proximal da tíbia uma cavidade de 3 mm, sendo implantada a HAD. Após a cirurgia os animais foram divididos em três grupos (n=5: controle (CT, anti-inflamatório não esteroidal (AINES e anti-inflamatório esteroidal (AIES. O grupo AINES foi submetido ao tratamento com cetoprofeno na dose de 12 mg/Kg/dia, o AIES recebeu doses de 0,10 mg/kg/dia de dexametasona o grupo CT recebeu solução fisiológica 0,9% (SF por via subcutânea durante 30 dias. Todos os grupos receberam a mesma dieta sólida e água ad libitum. Após 30 dias de experimento os animais sofreram eutanásia, os fêmures coletados, para teste mecânico, e os locais do implante das tíbias e o osso parietal, para análise histomorfométrica. Os grupos AINES e AIES apresentaram menor volume de osso neoformado na falha óssea e ao redor do implante de HAD , como também, menor força máxima para a ruptura completa dos fêmures, quando comparados com o grupo CT. O uso do cetoprofeno e a dexametasona interferiram na osteogênese ao redor do implante de HAD e no osso parietal, diminuindo a resistência óssea principalmente pela inibição da COX2 e diminuição das prostaglandinas, comprometendo a estabilidade e manutenção do implante.

The influence of cigarette smoke inhalation on bone density: a radiographic study in rats Influência da fumaça de cigarro na densidade óssea: estudo radiográfico em ratos

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João Batista César-Neto

2005-03-01

Full Text Available The aim of this study was to evaluate the influence of cigarette smoke inhalation (CSI and its cessation on tibiae bone quality. Forty-one male Wistar rats were randomly assigned to one of the following groups: Group 1 - control (n = 14, Group 2 - 3 months of CSI and 2 months without exposure to CSI (n = 12, and Group 3 - 5 months of CSI (n = 15. At the end of the experimental period, the animals were sacrificed, the tibiae removed and immediately radiographed for photodensitometric analysis. The results showed that continuous exposure to cigarette smoke promoted a significantly reduced bone density (p 0.05. Thus, within the limits of the present study, it can be concluded that CSI may affect tibiae bone quality, and CSI cessation results in a return towards the level of the control group.O objetivo deste estudo foi avaliar a influência da inalação da fumaça de cigarro (IFC e o efeito de sua interrupção na qualidade óssea da tíbia. Quarenta e um ratos Wistar machos foram aleatoriamente designados a um dos seguintes grupos: Grupo 1 - controle (n = 14, Grupo 2 - 3 meses de IFC e 2 meses sem exposição à fumaça (n = 12 e Grupo 3 - 5 meses de IFC (n = 15. Ao final do período experimental, os animais foram sacrificados, as tíbias removidas e imediatamente radiografadas para a análise fotodensitométrica. Os resultados mostraram que a exposição contínua à fumaça de cigarro promoveu uma significante redução na densidade óssea (p 0,05. Portanto, dentro dos limites do presente estudo, pode-se concluir que a IFC pode influenciar a qualidade óssea da tíbia e que a interrupção da inalação parece reverter esse efeito negativo resultando numa densidade óssea semelhante à do grupo controle.

Pawedness Trait Test (PaTRaT—A New Paradigm to Evaluate Paw Preference and Dexterity in Rats

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Ana M. Cunha

2017-10-01

Full Text Available In rodents, dexterity is commonly analyzed in preference paradigms in which animals are given the chance to use either the left or the right front paws to manipulate food. However, paw preference and dexterity at population and individual levels are controversial as results are incongruent across paradigms. We have therefore developed a semi-quantitative method—the pawdeness trait test (PaTRaT—to evaluate paw preference degree in rats. The PaTRaT consists in a classification system, ranging from +4 to −4 where increasingly positive and negative values reflect the bias for left or right paw use, respectively. Sprague-Dawley male rats were confined into a metal rectangular mesh cylinder, from which they can see, smell and reach sugared rewards with their paws. Due to its size, the reward could only cross the mesh if aligned with its diagonal, imposing additional coordination. Animals were allowed to retrieve 10 rewards per session in a total of four sessions while their behavior was recorded. PaTRaT was repeated 4 and 8 weeks after the first evaluation. To exclude potential bias, rats were also tested for paw fine movement and general locomotion in other behavioral paradigms as well as impulsivity (variable delay-to-signal, VDS, memory and cognitive flexibility (water maze. At the population level 54% of the animals presented a rightward bias. Individually, all animals presented marked side-preferences, >2 and <−2 for left- and right-sided bias, respectively, and this preference was stable across the three evaluations. Inter-rater consistency was very high between two experienced raters and substantial when two additional inexperienced raters were included. Left- and right-biased animals presented no differences in the ability to perform fine movements with any of the forelimbs (staircase and general locomotor performance. Additionally, these groups performed similarly in executive function and memory tasks. In conclusion, PaTRaT is able

Geografia e sport: per una definizione del rapporto tra spazio e società

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Rosario De Iulio

2013-04-01

Full Text Available Le finalità del presente saggio si possono sintetizzare in due differenti punti, ma strettamente legati tra di loro. Il primo è quello di illustrare, seppur brevemente, l’insieme delle teorie che hanno portato all’origine e alla affermazione della geografia dello sport, mentre il secondo cercherà di tracciare seppur a grandi linee, le emergenti linee di ricerca nell’ottica del complesso rapporto tra spazio e società. In tal modo si offriranno alcuni spunti di riflessione e nuove chiavi di lettura sul poliedrica relazione tra attività sportiva, intesa come fenomeno socio-culturale, e spazio/territorio, ormai sempre più centrale nelle più recenti politiche di pianificazione territoriale, considerando anche dell’attuale fase di deindustrializzazione che investe i nostri centri urbani.

Plasma rico em plaquetas para reparação de falhas ósseas em cães Platelet-rich plasma for canine bone restoration

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Anna Laeticia Trindade Barbosa

2008-08-01

Full Text Available As plaquetas chegam rapidamente ao local da ferida e liberam múltiplos fatores de crescimento (FC e citocinas que contribuem para a reparação óssea e aumentam a vascularização local. O Plasma Rico em Plaquetas (PRP concentra as plaquetas e os FC liberados por elas, aceleram a formação óssea e melhora a qualidade do trabeculado. Este trabalho apresenta um protocolo para confecção de PRP e demonstra alguns aspectos da sua utilização na reparação óssea de cães. O protocolo foi desenvolvido a partir de sangue coletado por punção jugular em três cães adultos, pesando em média 20kg. Para avaliação da aplicação clínica e dos aspectos da reparação óssea, foram criadas duas falhas mediais no terço proximal de cada tíbia. Assim, a falha 1 não foi preenchida, constituindo o controle, a falha 2 foi preenchida com 3mg de enxerto ósseo autógeno da crista da tíbia, a falha 3 com gel de plaquetas (PRP e falha a número 4 com a associação PRP e 3mg de enxerto ósseo autógeno. O protocolo laboratorial proposto mostrou-se de fácil execução e de baixo custo e possibilitou a concentração adequada de plaquetas no PRP final, cujo número foi dependente da contagem inicial no sangue total de cada animal. A comparação da radiopacidade na região da falha, em todos os tratamentos, e ao longo do tempo demonstrou que o PRP associado ao enxerto determinou maior precocidade e uniformidade de radiopacidade, quando comparado à falha preenchida pelo PRP e ao enxerto usados isoladamente, e sendo que ambos determinam melhores resultados de preenchimento que a falha mantida sem tratamento.The platelets arrive quickly at the injury site and release several growth factors (GF and citokines that contribute to bone repair and increasing local vascularization. The Platelet-rich Plasma (PRP concentrates the platelets and their growth factors, increasing the rate of bone formation and better quality of trabecular bone. This research presents

Bioanalytical system for detection of cancer cells with photoluminescent ZnO nanorods

Science.gov (United States)

Viter, R.; Jekabsons, K.; Kalnina, Z.; Poletaev, N.; Hsu, S. H.; Riekstina, U.

2016-11-01

Using photoluminescent ZnO nanorods and carbohydrate marker SSEA-4, a novel cancer cell recognition system was developed. Immobilization of SSEA-4 antibodies (αSSEA-4) on ZnO nanorods was performed in buffer solution (pH = 7.1) over 2 h. The cancer cell line probes were fixed on the glass slide. One hundred microliters of ZnO-αSSEA-4 conjugates were deposited on the cell probe and exposed for 30 min. After washing photoluminescence spectra were recorded. Based on the developed methodology, ZnO-αSSEA-4 probes were tested on patient-derived breast and colorectal carcinoma cells. Our data clearly show that the carbohydrate SSEA-4 molecule is expressed on cancer cell lines and patient-derived cancer cells. Moreover, SSEA-4 targeted ZnO nanorods bind to the patient-derived cancer cells with high selectivity and the photoluminescence signal increased tremendously compared to the signal from the control samples. Furthermore, the photoluminescence intensity increase correlated with the extent of malignancy in the target cell population. A novel portable bioanalytical system, based on optical ZnO nanorods and fiber optic detection system was developed. We propose that carbohydrate SSEA-4 specific ZnO nanorods could be used for the development of cancer diagnostic biosensors and for targeted therapy.

Maria Rizzarelli, Una terra che è solo visione. La poesia di Pasolini tra cinema e pittura

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Paolo Lago

2016-11-01

Full Text Available La contaminazione tra scrittura e visione, la «natura anfibologica» tra parola e immagine, secondo una definizione dello stesso autore, risulta fondamentale nell’intera opera letteraria di Pier Paolo Pasolini.

Zaustavljanje broda koji nije u vlasništvu osobe odgovorne za pomorsku tražbinu : [prikaz presude

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Jasenko Marin

2002-12-01

Full Text Available Načelno, privremeno zaustavljen može biti samo brod čiji je vlasnik protivnik osiguranja, odnosno osoba odgovorna za pomorsku tražbinu čije se osiguranje traži. U skladu s tim nije dopušteno zaustavljanje broda u vlasništvu trgovačkog društva čiji je jedini član osoba odgovorna za pomorsku tražbinu.

Emozionanti mostri femminili tra storia e cinema

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Angela Giallongo

2014-10-01

Full Text Available Ricorrendo al concetto chiave di paura, usato come categoria storica, il presente contributo esamina l’antico mito gorgonico in sette film e in alcuni esempi di Teen Movies. L’analisi critica focalizza l’attenzione sull’interdipendenza tra le emozioni negative, gli usi attuali di questa immagine archetipica e la ricerca storica sull’immaginario di genere.

Bone mineral density in postmenopausal women with and without breast cancer Densidade mineral óssea em mulheres na pós-menopausa com e sem câncer de mama

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Délio Marques Conde

2012-12-01

Full Text Available OBJECTIVE: The values of bone mineral density (BMD were compared in postmenopausal women with and without breast cancer. METHODS: A cross-sectional study was conducted, including 51 breast cancer survivors (BCS and 71 women without breast cancer, who were non-users of hormone therapy, tamoxifen, or aromatase inhibitors. BMD T-scores and measurements in grams per centimeter squared (g/cm² were obtained at the femoral neck, trochanter, Ward's triangle, and lumbar spine. Osteopenia and osteoporosis were grouped and categorized as abnormal BMD. Unconditional logistic regression analysis was used to estimate the odds ratios (OR of abnormal BMD values as measures of association, with 95% confidence intervals (CIs, adjusting for age, years since menopause, parity, and body mass index (BMI. RESULTS: The mean age of the women with and without breast cancer was 54.7 ± 5.8 years and 58.2 ± 4.8 years (p OBJETIVO: Comparar a densidade mineral óssea (DMO de mulheres na pós-menopausa com e sem câncer de mama. MÉTODOS: Conduziu-se estudo de corte transversal, incluindo 51 mulheres com câncer de mama e 71 mulheres sem câncer de mama, não usuárias de terapia hormonal, tamoxifeno ou de inibidores da aromatase. Avaliou-se a DMO, em T-score e em gramas por centímetro quadrado (g/cm², no colo do fêmur, trocânter, triângulo de Wards e na coluna lombar. Osteopenia e osteoporose foram agrupadas e categorizadas como DMO alterada. Utilizou-se a análise de regressão logística não condicional para estimar o odds ratios (OR de DMO alterada como medida de associação, com intervalo de confiança de 95% (IC 95%, ajustando-se por idade, anos de menopausa, paridade e índice de massa corpórea (IMC. RESULTADOS: A média de idade de mulheres com e sem câncer de mama foi 54,7 ± 5,8 anos e 58,2 ± 4,8 anos (p < 0,01, respectivamente. Após ajustar por idade, paridade e IMC, DMO alterada no colo do fêmur (OR ajustado: 4,8; IC 95%: 1,5-15,4, trocânter (OR

27 CFR 4.60 - Application.

Science.gov (United States)

2010-04-01

... THE TREASURY LIQUORS LABELING AND ADVERTISING OF WINE Advertising of Wine § 4.60 Application. No... disseminated by radio or television broadcast, or in any newspaper, periodical, or any publication, by any sign... advertising is in, or is calculated to induce sale in, interstate or foreign commerce, or is disseminated by...

33 CFR 1.05-60 - Negotiated rulemaking.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Negotiated rulemaking. 1.05-60... committee members will negotiate in good faith; (4) There is a likelihood of a committee consensus in a... Coast Guard has resources to do negotiated rulemaking; and (7) The Coast Guard can use the consensus of...

Immune Curbing of Cancer Stem Cells by CTLs Directed to NANOG

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Christina Wefers

2018-06-01

Full Text Available Cancer stem cells (CSCs have been identified as the source of tumor growth and disease recurrence. Eradication of CSCs is thus essential to achieve durable responses, but CSCs are resistant to current anti-tumor therapies. Novel therapeutic approaches that specifically target CSCs will, therefore, be crucial to improve patient outcome. Immunotherapies, which boost the body’s own immune system to eliminate cancerous cells, could be an alternative approach to target CSCs. Vaccines of dendritic cells (DCs loaded with tumor antigens can evoke highly specific anti-tumor T cell responses. Importantly, DC vaccination also promotes immunological memory formation, paving the way for long-term cancer control. Here, we propose a DC vaccination that specifically targets CSCs. DCs loaded with NANOG peptides, a protein required for maintaining stem cell properties, could evoke a potent anti-tumor immune response against CSCs. We hypothesize that the resulting immunological memory will also control newly formed CSCs, thereby preventing disease recurrence.

The Evolution of NR TrA (Nova TrA 2008) from 2008 through 2017

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Walter, Frederick M.; Burwitz, Vadim; Kafka, Stella

2018-06-01

The classical nova NR TrA was discovered as an O-type optically-thick classical nova. There is no evidence that it formed dust. Within four years the envelope became sufficiently thin to reveal an eclipsing accretion disk-dominated system with orbitally-modulated permitted lines of C IV, N V, and O VI. XMM observations reveal a non-eclipsing soft X-ray source and a deeply-eclipsing UV continuum. We will present the first ten years of optical spectral evolution of this system accompanied by ten years of BVRIJHK photometry, with an eye to deciphering the current nature of the system.

Potencial evocado auditivo de tronco encefálico por via óssea em indivíduos com perda auditiva sensorioneural

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Luciana Castelo Branco Camurça Fernandes

2013-06-01

Full Text Available OBJETIVO: caracterizar os resultados do PEATE por via óssea em indivíduos com perda auditiva sensorioneural leve, comparando esses dados com o grupo controle, formado por indivíduos audiologicamente normais. MÉTODO: a amostra foi constituída por 40 adultos, de ambos os sexos, com faixa etária de 18 a 55 anos, distribuídos em um grupo controle de 30 indivíduos com audição normal e um grupo estudo composto de 10 indivíduos com perda auditiva sensorioneural de grau leve. O PEATE foi realizado com equipamento EP15, da marca Interacoustics. O estímulo utilizado foi o clique com taxa de apresentação de 27,7/s, em um total de 2000 estímulos, com polaridade de rarefação por VA e alternada para VO e filtro passa-banda de 50Hz e 3000Hz. RESULTADOS: em indivíduos com perda sensorioneural de grau leve, não houve diferenças estatisticamente significantes entre o limiar do PEATE por via aérea e óssea, estando esses limiares equivalentes, com GAP aéreo-ósseo menor que 10dB. A latência da onda V no limiar eletrofisiológico e a 50 dBnNA foram menores que as referidas latências observadas em indivíduos com audição normal. CONCLUSÃO: foram encontrados limiares eletrofisiológicos por via óssea equivalente aos limiares obtidos por via aérea, com presença de GAP aéreo-ósseo menor que 10dBnNA. Assim a utilização do PEATE por VO fornece dados para uma caracterização mais detalhada do tipo da perda auditiva.

ART, Stoffenmanager, and TRA: A Systematic Comparison of Exposure Estimates Using the TREXMO Translation System.

Science.gov (United States)

Savic, Nenad; Gasic, Bojan; Vernez, David

2017-12-15

Several occupational exposure models are recommended under the EU's REACH legislation. Due to limited availability of high-quality exposure data, their validation is an ongoing process. It was shown, however, that different models may calculate significantly different estimates and thus lead to potentially dangerous conclusions about chemical risk. In this paper, the between-model translation rules defined in TREXMO were used to generate 319000 different in silico exposure situations in ART, Stoffenmanager, and ECETOC TRA v3. The three models' estimates were computed and the correlation and consistency between them were investigated. The best correlated pair was Stoffenmanager-ART (R, 0.52-0.90), whereas the ART-TRA and Stoffenmanager-TRA correlations were either lower (R, 0.36-0.69) or no correlation was found. Consistency varied significantly according to different exposure types (e.g. vapour versus dust) or settings (near-field versus far-field and indoors versus outdoors). The percentages of generated situations for which estimates differed by more than a factor of 100 ranged from 14 to 97%, 37 to 99%, and 1 to 68% for Stoffenmanager-ART, TRA-ART, and TRA-Stoffenmanager, respectively. Overall, the models were more consistent for vapours than for dusts and solids, near-fields than for far-fields, and indoor than for outdoor exposure. Multiple linear regression analyses evidenced the relationship between the models' parameters and the relative differences between the models' predictions. The relative difference can be used to estimate the consistency between the models. Furthermore, the study showed that the tiered approach is not generally applicable to all exposure situations. These findings emphasize the need for a multiple-model approach to assessing critical exposure scenarios under REACH. Moreover, in combination with occupational exposure measurements, they might also be used for future studies to improve prediction accuracy. © The Author(s) 2017

Crystal and molecular structure of new fullerides (Ph4P)2C60Hal (Hal = Br, I) and (Ph4As)2C60Cl

International Nuclear Information System (INIS)

Gritsenko, V.V.; D'yachenko, O.A.; Shilov, G.V.; Spitsyn, N.G.; Yagubskij, E h.B.

1997-01-01

New stable on air fullerides (Ph 4 P) 2 C 60 Hal (Hal = Br, I) and (Ph 4 As) 2 C 60 Cl were synthesized and their crystal structure were determined. On the basis of their relative crystallochemical analysis fullerides obtained were shown to be isostructural. They are described by general formula (Ph 4 X) 2 C 60 Hal (X = H, As; Hal = Cl, Br, I) and crystallized in triclinic system. Anions C 60 - and Hal - occupy the centre-symmetrical positions, cations (PH 4 P) + and (Ph 4 As) + occupy the general positions. The main parameters of molecular structure are presented

HWMA/RCRA Closure Plan for the TRA Fluorinel Dissolution Process Mockup and Gamma Facilities Waste System

International Nuclear Information System (INIS)

K. Winterholler

2007-01-01

This Hazardous Waste Management Act/Resource Conservation and Recovery Act closure plan was developed for the Test Reactor Area Fluorinel Dissolution Process Mockup and Gamma Facilities Waste System, located in Building TRA-641 at the Reactor Technology Complex (RTC), Idaho National Laboratory Site, to meet a further milestone established under the Voluntary Consent Order SITE-TANK-005 Action Plan for Tank System TRA-009. The tank system to be closed is identified as VCO-SITE-TANK-005 Tank System TRA-009. This closure plan presents the closure performance standards and methods for achieving those standards

Análise clínica e epidemiológica do transplante de medula óssea em um serviço de oncologia pediátrica Clinical and epidemiological analysis of bone marrow transplantation in a pediatric oncology unit

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Cláudio Galvão de Castro Jr.

2003-10-01

Full Text Available OBJETIVOS: Descrever o perfil e as complicações agudas mais importantes das crianças que receberam transplante de medula óssea no serviço de oncologia pediátrica do Hospital de Clínicas de Porto Alegre. CASUÍSTICA E MÉTODOS: Análise retrospectiva de 41 pacientes, menores de 21 anos, transplantados entre agosto de 1997 até junho de 2002. Deste total, 20 pacientes receberam transplante alogênico, e 21 transplante autogênico. RESULTADOS: No transplante de medula óssea alogênico, a média de idade foi de 8,9 + 5,4 anos, sendo 12 pacientes do sexo masculino. As fontes de células foram: medula óssea, 12; sangue periférico, 5; e sangue de cordão umbilical não aparentado, 3. As doenças tratadas foram: leucemia linfóide aguda em 7 pacientes, leucemia mielóide crônica em 2, leucemia mielóide aguda em 4, síndrome mielodisplásica em 2, linfoma de Burkitt em 1, anemia aplástica grave em 1, anemia de Fanconi em 1, síndrome Chediak-Higashi em 1, e imunodeficiência congênita combinada grave em 1. Um paciente desenvolveu doença do enxerto contra hospedeiro aguda grau 2, três pacientes grau 4, e outros três desenvolveram doença do enxerto contra hospedeiro crônica. Todos haviam recebido sangue periférico como fonte de células. A sobrevida global aos 4 anos foi de 70,0 + 10,3%. A principal causa do óbito foi doença do enxerto contra hospedeiro, em três pacientes, e sepse, em outros três. Todos os óbitos ocorreram antes do centésimo dia. Um dos pacientes que recebeu sangue de cordão umbilical não aparentado está vivo, em bom estado e sem uso de medicações 3 anos e 6 meses pós-transplante. No transplante de medula óssea autogênico, a média de idade foi de 8,7 + 4,3 anos, sendo 11 pacientes do sexo masculino. As fontes de células foram: sangue periférico, 16; medula óssea, 3; sangue periférico mais medula óssea, 2. As doenças tratadas foram: tumor de Wilms em 5 pacientes, tumores da família do sarcoma de

Demethylating agent, 5-azacytidine, reverses differentiation of embryonic stem cells

International Nuclear Information System (INIS)

Tsuji-Takayama, Kazue; Inoue, Toshiya; Ijiri, Yoshihiro; Otani, Takeshi; Motoda, Ryuichi; Nakamura, Shuji; Orita, Kunzo

2004-01-01

The de novo methylation activity is essential for embryonic development as well as embryonic stem (ES) cell differentiation, where the intensive and extensive DNA methylation was detected. In this study, we investigated the effects of a demethylating agent, 5-azacytidine (5-AzaC), on differentiated ES cells in order to study the possibility of reversing the differentiation process. We first induced differentiation of ES cells by forming embryoid bodies, and then the cells were treated with 5-AzaC. The cells showed some undifferentiated features such as stem cell-like morphology with unclear cell-to-cell boundary and proliferative responsiveness to LIF. Moreover, 5-AzaC increased the expressions of ES specific markers, SSEA-1, and alkaline phosphatase activity as well as ES specific genes, Oct4, Nanog, and Sox2. We also found that 5-AzaC demethylated the promoter region of H19 gene, a typical methylated gene during embryonic differentiation. These results indicate that 5-AzaC reverses differentiation state of ES cells through its DNA demethylating activity to differentiation related genes

The Thrombin Receptor Antagonist for Clinical Event Reduction in Acute Coronary Syndrome (TRA*CER) trial: study design and rationale.

Science.gov (United States)

2009-09-01

The protease-activated receptor 1 (PAR-1), the main platelet receptor for thrombin, represents a novel target for treatment of arterial thrombosis, and SCH 530348 is an orally active, selective, competitive PAR-1 antagonist. We designed TRA*CER to evaluate the efficacy and safety of SCH 530348 compared with placebo in addition to standard of care in patients with non-ST-segment elevation (NSTE) acute coronary syndromes (ACS) and high-risk features. TRA*CER is a prospective, randomized, double-blind, multicenter, phase III trial with an original estimated sample size of 10,000 subjects. Our primary objective is to demonstrate that SCH 530348 in addition to standard of care will reduce the incidence of the composite of cardiovascular death, myocardial infarction (MI), stroke, recurrent ischemia with rehospitalization, and urgent coronary revascularization compared with standard of care alone. Our key secondary objective is to determine whether SCH 530348 will reduce the composite of cardiovascular death, MI, or stroke compared with standard of care alone. Secondary objectives related to safety are the composite of moderate and severe GUSTO bleeding and clinically significant TIMI bleeding. The trial will continue until a predetermined minimum number of centrally adjudicated primary and key secondary end point events have occurred and all subjects have participated in the study for at least 1 year. The TRA*CER trial is part of the large phase III SCH 530348 development program that includes a concomitant evaluation in secondary prevention. TRA*CER will define efficacy and safety of the novel platelet PAR-1 inhibitor SCH 530348 in the treatment of high-risk patients with NSTE ACS in the setting of current treatment strategies.

Influence of image filters on the reproducibility measurements of alveolar bone loss Influência dos filtros de imagem sobre a reprodutibilidade de medidas da perda óssea alveolar

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Wivian Eiras Garcia Wong Alves

2006-12-01

Full Text Available The reproducibility of measurements of alveolar bone loss on radiographs may be a problem on epidemiologic studies, as they are based on comparisons of the diagnosis of various examiners. The aim of the present research paper was to assess the inter- and intra-examiner reproducibility of measurements of the interproximal alveolar bone loss on non-manipulated digital radiographs and after the application of image filters. Five Oral Radiologists measured the distance between the cementoenamel junction (CEJ to the alveolar crest or to the deepest point of the bony defect on 12 interproximal digital radiographs of molars and bicuspids of a dry human skull. The digital manipulation and the linear measurements were obtained with the Trophy Windows software (Throphy®. For each image, six different versions were created: 1 non-manipulated; 2 bright-contrast adjustment; 3 negative; 4 negative with brightness-contrast adjustment; 5 pseudo-colored; 6 pseudo-colored with brightness-contrast adjustment. In order to prevent interpretation bias because of the repetition of measurements, the examiners measured the radiographs in a random sequence. The two-way ANOVA test at 5% level of significance to compare the means of readings of the same operator with each filter indicated p0.05 for all filters. Based on the results, we concluded that linear measurements of interproximal alveolar bone loss on digital radiographs are highly reproducible among examiners. Nevertheless, the application of image filters significantly influenced the degree of intra-examiner reproducibility. Some filters even reduced the reproducibility of intra-examiner readings.A reprodutibilidade de medidas da perda óssea alveolar em radiografias pode se tornar um problema em levantamentos epidemiológicos, que são baseados em comparações de diagnóstico de vários observadores. O objetivo neste estudo foi avaliar a reprodutibilidade intra e inter-examinador de medidas da perda óssea

Como a célula óssea reconhece o estímulo mecânico? How do bone cells sense mechanical loading?

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Carlos Vinícius Buarque de Gusmão

2009-01-01

Full Text Available Sob a influência da gravidade, o tecido ósseo sofre maior ou menor deformação de acordo com a intensidade das atividades da vida diária. Sabe-se que as atividades que resultam em impacto são as que mais estimulam a osteogênese e assim reduzem a perda de massa óssea. Conhecer como as células ósseas reconhecem a deformação mecânica imposta ao osso e iniciam uma série de reações bioquímicas em cadeia é de fundamental importância para o desenvolvimento de práticas terapêuticas e preventivas na atividade ortopédica. Ainda há um longo caminho para o entendimento de todo esse processo, mas o conhecimento atual progrediu bastante e há pesquisas com finalidade terapêutica. O sinal mecânico para ser transformado em biológico (mecanotransdução deve ser amplificado no nível celular pela estrutura histológica do tecido ósseo, gerando tensões em proteínas da membrana celular (integrinas e alterando a estrutura espacial dessas proteínas. Essa alteração ativa ligações entre elas e o citoesqueleto, originando as adesões focais, locais onde proteínas citoplasmáticas são recrutadas para facilitar as reações bioquímicas. A quinase de adesão focal (FAK é a principal delas, sendo autoativada após sofrer alteração estrutural pelas integrinas. A FAK ativada incita reações em cascata, resultando na ativação da ERK-1/2 e da Akt, proteínas que, junto com a FAK, regulam a produção da massa óssea. Acredita-se que o osteócito seja a célula óssea responsável por reconhecer o estímulo mecânico e transmiti-lo aos osteoblastos e osteoclastos. Canais iônicos e gap junctions são cogitados como meios de comunicação intercelular para a transmissão bioquímica do estímulo mecânico. Esses eventos ocorrem continuamente no tecido ósseo e regulam a remodelação óssea.Influenced by gravidity, bone tissue experiences stronger or lighter deformation according to the strength of the activities of daily life

Densidade mineral óssea em crianças: associação com dor músculo-esquelética e/ou hipermobilidade articular

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Roberto Adriana Madureira

2002-01-01

Full Text Available Introdução: a hipermobilidade articular pode estar associada à dor músculo-esquelética. A relação da hipermobilidade com a redução da densidade mineral óssea ainda é desconhecida. Existem relatos de osteoporose em associação com doenças genéticas que cursam com hipermobilidade articular. O nosso objetivo foi detectar a possível associação entre hipermobilidade articular e alterações na densidade mineral óssea (DMO em crianças com e sem dor músculo-esquelética. Casuística e métodos: foram avaliadas 93 crianças, com idade entre 5 e 10 anos, quanto à presença de hipermobilidade articular e quanto à presença de dor músculo-esquelética, através de questionário dirigido aos pais. Todas as crianças realizaram densitometria óssea de coluna lombar ao nível das vértebras L2-L4. Resultados: as crianças foram distribuídas de acordo com a presença ou não de hipermobilidade articular associada ou não à dor músculo-esquelética: 29 (31,2% com hipermobilidade e com dor músculo-esquelética, 20 (21,5% com hipermobilidade e sem dor, 22 (23,6% sem hipermobilidade e com dor e 22 (23,6% sem hipermobilidade e sem dor (grupo controle. Vinte e quatro (25,8% crianças apresentaram perda de DMO maior que 10% com relação à DMO adequada para a idade e sexo. A DMO mostrou-se significantemente menor em relação ao grupo controle nos grupos: com hipermobilidade (independente da presença de dor, com dor (independente da presença de hipermobilidade, com hipermobilidade e sem dor e sem hipermobilidade e com dor. Conclusão: a DMO pode estar diminuída em crianças com hipermobilidade (independente da presença de dor músculo-esquelética e em crianças com dor (independente da presença de hipermobilidade em relação aos controles.

Efeitos da natação e do treinamento resistido na densidade mineral óssea de mulheres idosas Effects of swimming and resistance training on bone mineral density of older women

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Carlos Kemper

2009-02-01

Full Text Available Exercícios com impacto como caminhada, saltos, corridas e exercícios resistidos são muito utilizados para prevenção da perda óssea em idosas. No entanto, poucos são os estudos que relatam os efeitos da natação na manutenção da massa óssea em mulheres idosas. Portanto, o objetivo deste estudo foi comparar os efeitos da natação com o treinamento resistido na densidade mineral óssea (DMO de mulheres idosas. Vinte e três mulheres com idade média de 63,9 ± 6,49 anos foram divididas em dois grupos: 1 grupo natação (NAT, n = 13, que treinou em intensidade entre 60 e 90% da freqüência cardíaca de reserva; 2 grupo treinamento resistido (TR, n = 10, que treinou os principais grupamentos musculares com três séries a 80% de 1RM. Os dois grupos praticaram três vezes por semana com uma hora de duração para cada sessão, durante seis meses. A DMO do colo do fêmur e da coluna lombar (L2-L3-L4 foi mensurada através de DXA antes (T0 e após seis meses de treino (T6. Os resultados mostraram que as médias para a DMO lombar em T0 (0,9250 ± 0,1506g/cm² e T6 (0,9303 ± 0,1269g/cm² para o NAT e em T0 (0,9739 ± 0,1249g/cm² e T6 (0,9737 ± 0,1317g/cm² para o TR não foram diferentes quando comparadas intra ou intergrupos. De modo similar, não houve diferenças entre a DMO do colo do fêmur em T0 (0,7784 ± 0,1523g/cm² e T6 (0,7905 ± 0,1610g/cm² para o NAT e T0 (0,7546 ± 0,1360g/cm² e T6 (0,7522 ± 0,1421g/cm² para o TR. Os resultados deste estudo não demonstraram diferenças na DMO entre NAT e TR após seis meses de treino; e que tanto TR quanto NAT não produzem aumentos significativos na DMO de mulheres idosas nesse período.Impact exercises like walking, jumping, running and resistance exercises are largely used to prevent bone loss in older women; however, few studies report the benefits of swimming to bone tissue. Therefore, the purpose of this study was to compare the effects of swimming with resistance training on bone

O papel do Fator de Necrose Tumoral Alfa (TNF-alfa no processo de erosão óssea presente no colesteatoma adquirido da orelha média The role of Tumor Necrosis Factor -Alpha (TNF- alpha in bone resorption present in middle ear cholesteatoma

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Rodrigo Faller Vitale

2007-02-01

Full Text Available O colesteatoma adquirido da orelha média causa erosão óssea, com altas taxas de morbidade e mortalidade. O TNF-alfa (TNF-alfa lambda uma das principais citocinas envolvidas neste processo. OBJETIVO: Avaliar o papel do TNF-alfa na reabsorsão óssea e a ação dele no colesteatoma. MATERIAL E MÉTODOS: Foi realizado um levantamento e uma revisão crítica da literatura. RESULTADOS: Todos os autores estudados concordam com a importância do TNF-alfa no processo de reabsorção óssea presente no colesteatoma e com o grau de destruição observado. Diferentes trabalhos demonstraram que o TNF-alfa é capaz de provocar erosão óssea, através de diferentes vias de ação. Ele pode estimular a diferenciação e a maturação dos osteoclastos ou, ainda, agir na matriz óssea expondo-a à ação dos osteoclastos. Existe a possibilidade de inibir a ação do TNF-alfa, diminuindo seus efeitos e prevenindo a perda óssea em doenças como a artrite reumatóide. Não existe, entretanto, trabalhos específicos em colesteatoma. Não existe consenso sobre a sua localização. Estas diferenças, provavelmente, ocorrem devido à distribuição dos receptores. CONCLUSÃO: O TNF-alfa, presente no colesteatoma promove a reabsorsão óssea, juntamente com outras citocinas (RANKL e IL-1, estando relacionado com a presença de complicações.Cholesteatoma may cause bone erosion, with high morbidity and mortality rates. Tumor Necrosis Factor -Alpha (TNF-a is one of the main cytokines involved in this process. Our goal was to evaluate the role of TNF-a in Bone Resorption and its effect on cholesteatoma. MATERIAL AND METHODS: analysis and critical literature review. RESULTS: Different studies have demonstrated that TNF-a is capable of causing bone erosion. It may stimulate the differentiation and maturation of osteoclasts or it may act on the bone matrix, exposing it to the action of the osteoclasts. It is possible to inhibit TNF-a, reducing its effects and prevent

Uso da biocerâmica no preenchimento de falhas ósseas Use of bioceramics in filling bone defects

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Carlos Antônio Garrido

2010-01-01

Full Text Available OBJETIVO: Apresentar os resultados encontrados com o uso da biocerâmica no preenchimento das falhas ósseas decorrentes de lesões traumáticas ou ortopédicas. MÉTODO: Foram avaliados 36 pacientes, portadores de falhas ósseas de etiologia pós-traumática ou ortopédica, 19 pacientes do sexo masculino (52,8% e 17 do sexo feminino (47,2%. A idade variou de 19 a 84 anos, com média de 45,7 anos e mediana de 37 anos. Foram incluídos apenas os pacientes com falhas que necessitaram, no mínimo, cinco gramas de biocerâmica. Foram classificadas como ortopédicas o total de 18 casos; as falhas ósseas observadas nas revisões de artroplastias totais do quadril, 11 casos; artroplastia total do quadril, primária, por coxartrose, um caso; osteotomias de fêmur ou tíbia de cunha aberta, cinco casos, e artrodese do tarso, um caso. Como falhas pós-traumática, 18 casos; as pseudoartroses não infectadas, oito casos; fraturas recentes do planalto tibial com compressão do osso esponjoso, três casos; fraturas expostas tratadas com fixadores externos, sete casos. A técnica cirúrgica utilizada foi o de curetar e desbridar a lesão até se encontrar osso de aspecto viável a enxertia, a seguir utilizou-se a biocerâmica para preenchimento da falha e algum tipo de fixação. RESULTADO: Dos 36 pacientes avaliados, observou-se que 35 (97,2% apresentaram integração da biocerâmica, um caso de fratura exposta tratada com fixador externo apresentou integração deficiente da biocerâmica. CONCLUSÃO: O tratamento de falhas ósseas de etiologia pós-traumática ou ortopédica, com o uso da cerâmica fosfocálcica composta de hidroxiapatita, se revelou um método prático, eficaz e seguro.OBJETIVE: To present the results of the use of biological ceramic in filling bone defects resulting from traumatic or orthopedic injuries. METHODS: We evaluated 36 patients with bone defects caused by trauma or orthopedic injury. Nineteen patients were male (52.8% and 17

Associação de força e nível de atividade física à densidade mineral óssea na pós-menopausa

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Cristiane Fialho Ferreira da Silva

2015-04-01

Full Text Available INTRODUÇÃO: o exercício físico atua melhorando a densidade mineral óssea DMO por gerar deformidades nesse tecido e estimular remodelação. OBJETIVO: verificar a associação entre força muscular e nível de atividade física à densidade mineral óssea DMO utilizando testes de força de bíceps, de sentar e levantar, dinamometria de mãos, história pregressa de atividade física e nível de atividade física, avaliado pelo pedômetro com a DMO em mulheres na pós-menopausa. MÉTODOS: estudo transversal, descritivo que mensurou a densidade mineral óssea pela absorção de dupla energia de raios X DXA da coluna lombar L1-L4, fêmur e antebraços em 62 mulheres saudáveis no pós-menopausa, com média de 56,82 ± 4,02 anos de idade. Foi aplicado questionário para atividade física pregressa e realizada contagem diária de passos pedômetro. A força muscular foi medida pelos testes de dinamometria de mãos, de 30 segundos de bíceps bilateral e de sentar e levantar da cadeira em 30 segundos. Realizou-se registro alimentar de três dias para mensuração da ingestão diária de cálcio e vitamina D. RESULTADOS: os indivíduos apresentaram altos níveis de atividade física, porém baixa ingestão diária de cálcio e vitamina D. Não foi verificada diferença estatisticamente significativa entre força muscular nos dois grupos estudados, com DMO diminuída e DMO normal. O grupo com DMO diminuída apresentou maior número de passos diários e menor peso quando comparado com o grupo com DMO normal. Foram observadas várias correlações positivas de baixa magnitude, embora significantes, entre a densitometria e a força muscular p < 0,50. CONCLUSÃO: os autores descreveram risco cinco vezes e meia maior em mulheres não praticantes de atividade física da adolescência até a idade adulta e de apresentarem redução da DMO em comparação com as mulheres que apresentaram DMO normal.

SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion

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Ming-Bao Ye

2017-06-01

Full Text Available Objective: To study the SDF-1/CXCR4 expression in bladder cancer tissue and the correlation with negative costimulatory molecule PD-L1, cell apoptosis and invasion. Methods: A total of 118 cases of bladder cancer tissue and para-carcinoma tissue surgically removed in our hospital between May 2014 and May 2016 were selected as the research samples, the RNA was extracted and then reverse-transcribed into cDNA, and the expression levels of SDF-1/ CXCR4, PD-L1/PD-1, cell apoptosis-related molecules and cell invasion-related molecules were detected. Results: SDF-1 and CXCR4 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue; PD-L1, PD-1, Rec1, Survivin, MRPS5, Nanog, BCAPP2Ac, TRPM8, TRPV2, ILK, β-catenin and GUGBP1 mRNA expression in bladder cancer tissue were significantly higher than those in para-carcinoma tissue and positively correlated with SDF-1 and CXCR4 mRNA expression. Conclusion: Highly expressed SDF-1/CXCR4 in bladder cancer tissue are closely related to the high expression of negative costimulatory molecule PD-L1, pro-proliferation molecules and proinvasion molecules, and SDF-1/CXCR4 can promote the immune escape, proliferation and invasion of bladder cancer cells.

Desenvolvimento de um modelo experimental de falha óssea infectada na ulna de coelhos Development of an experimental model of infected bone void in the ulna of rabbits

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Matheus Lemos Azi

2012-01-01

Full Text Available OBJETIVO: Desenvolver um modelo experimental que permita estudar a regeneração de grandes falhas ósseas em condições de infecção. MÉTODO: Falhas ósseas segmentares de 15mm foram criadas cirurgicamente na ulna de 12 coelhos e inoculadas com 5x10(8 unidades formadoras de colônia (UFC de S. aureus. O desbridamento da infecção foi realizado duas semanas após, seguida da aplicação sistêmica de gentamicina por quatro semanas. Os animais foram acompanhados por um período de 12 semanas para avaliação do controle da infecção e da regeneração óssea. RESULTADOS: A regeneração espontânea foi inferior a 25% do defeito na avaliação radiográfica e histológica. CONCLUSÃO: A Falha óssea infectada de 15mm na ulna de coelhos é incapaz de alcançar a regeneração completa sem tratamentos adicionais. Nível de Evidência V, Estudo experimental.OBJECTIVE: Develop a model that allowed the study of bone regeneration in infection conditions. METHOD: A 15 mm defect was surgically created in the rabbit ulna and inoculated with 5x10(8 colony-forming units (CFU of S. aureus. Surgical debridement was performed two weeks after and systemic gentamicin was administered for four weeks. Animals were followed up to 12 weeks to evaluate infection control and bone regeneration. RESULT: Bone regeneration was inferior to 25% of the defect in radiological and histological analysis. CONCLUSION: Infected bone defect of 15 mm in the rabbit ulna was unable to achieve full regeneration without further treatment. Level of Evidence V, Experimental Study.

15 CFR 60.1 - Public information.

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Public information. 60.1 Section 60.1 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade BUREAU OF THE CENSUS, DEPARTMENT OF COMMERCE PUBLIC INFORMATION § 60.1 Public information. The rules and procedures regarding...

Integrated approach for disease management and growth enhancement of Sesamum indicum L. utilizing Azotobacter chroococcum TRA2 and chemical fertilizer.

Science.gov (United States)

Maheshwari, D K; Dubey, R C; Aeron, Abhinav; Kumar, Bhavesh; Kumar, Sandeep; Tewari, Sakshi; Arora, Naveen Kumar

2012-10-01

Azotobacter chroococcum TRA2, an isolate of wheat rhizosphere displayed plant growth promoting attributes including indole acetic acid, HCN, siderophore production, solubilization of inorganic phosphate and fixation of atmospheric nitrogen. In addition, it showed strong antagonistic effect against Macrophomina phaseolina and Fusarium oxysporum. It also caused degradation and digestion of cell wall components, resulting in hyphal perforations, empty cell (halo) formation, shrinking and lysis of fungal mycelia along with significant degeneration of conidia. Fertilizer adaptive variant strain of A. chroococcum TRA2 was studied with Tn5 induced streptomycin resistant transconjugants of wild type tetracycline-resistant TRA2 (designated TRA2(tetra+strep+)) after different durations. The strain was significantly competent in rhizosphere, as its population increased by 15.29 % in rhizosphere of Sesamum indicum. Seed bacterization with the strain TRA2 resulted in significant increase in vegetative growth parameters and yield of sesame over the non-bacterized seeds. However, application of TRA2 with half dose of fertilizers showed sesame yield almost similar to that obtained by full dose treatment. Moreover, the oil yield increased by 24.20 %, while protein yield increased by 35.92 % in treatment receiving half dose of fertilizer along with TRA2 bacterized seeds, as compared to untreated control.

A influência da deficiência estrogênica no processo de remodelação e reparação óssea Effect of estrogen deficiency on bone turnover and bone repair

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Susana Ungaro Amadei

2006-02-01

Full Text Available INTRODUÇÃO: Nestes últimos anos, descobriu-se a complexidade dos mecanismos que influenciam a atividade óssea, e grande parte das pesquisas direcionou-se para o estudo de fatores capazes de modular as funções ósseas. Essa expansão da pesquisa deve-se, em parte, ao reconhecimento da osteoporose como importante problema na velhice. A osteoporose constitui uma das osteopatias mais comuns, caracterizando-se pela redução da massa óssea, determinada, por sua vez, pelo desequilíbrio entre reabsorção e neoformação. OBJETIVO: Apresentar uma revisão da literatura sobre os principais aspectos da remodelação e da reparação associados à deficiência estrogênica. Remodelação óssea: O osso apresenta processo contínuo de remodelação, entretanto anormalidades nesse processo ocorrem em algumas doenças, entre elas a osteoporose, sendo que a deficiência estrogênica parece ter o papel principal na sua gênese. Reparação óssea: Tal processo envolve uma cascata complexa de respostas biológicase, assim como a remodelação, é afetado por fatores locais e externos e regulado pela interação de diferentes mecanismos. Portanto, o aumento ou o decréscimo da capacidade de reparação óssea têm sido relacionados a alterações ocorridas na remodelação. Deficiência estrogênica e metabolismo ósseo: A maioria dos autores sugere uma redução na capacidade de remodelação e de reparação do tecido. DISCUSSÃO: Ainda não está determinado qual estágio da reparação é mais alterado, se a fase inicial de formação do calo ósseo, se a de mineralização ou se a fase tardia da reparação, a remodelação óssea. CONCLUSÃO: Como os mecanismos fisiológicos e a patogênese das alterações ósseas causadas pela deficiência estrogênica não estão completamente estabelecidos, novas pesquisas ainda são necessárias.INTRODUCTION: In the past few years, it was recognized the great complexity of the mechanisms that influence bone

Estudo dos limiares eletrofisiológicos das vias aérea e óssea em crianças com até 2 meses de idade Electrophysiologic threshold study in air and bone conduction in children with 2 months or less age

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Silvia Nápole Fichino

2007-04-01

Full Text Available O diagnóstico diferencial de perdas auditivas com potencial evocado auditivo de tronco encefálico por via aérea e por via óssea em crianças pequenas tem sido pouco estudado no Brasil. OBJETIVO: Comparar as respostas do potencial evocado auditivo de tronco encefálico por vias aérea e óssea em crianças de até 2 meses de idade sem perdas auditivas. FORMA DE ESTUDO: Clínico prospectivo com coorte transversal. MATERIAL E MÉTODO: Foram avaliadas 12 crianças que passaram na triagem auditiva, por meio do potencial evocado auditivo de tronco encefálico por via aérea e via óssea. A via óssea foi realizada sem mascaramento contralateral. As respostas foram comparadas e analisadas por meio do teste de McNemar e pela análise de variância com medidas repetidas. RESULTADOS: Não houve diferença estatística no limiar eletrofisiológico por via aérea e via óssea (p>0,05. O tempo de latência por via óssea foi estatisticamente maior do que o tempo de latência por via aérea (p=0,000. CONCLUSÃO: Houve concordância no registro do Potencial Evocado Auditivo de Tronco Encefálico captado por vias aérea e óssea nas intensidades próximas ao limiar auditivo; a latência da onda V registrada por via óssea foi estatisticamente maior que a registrada por via aérea.The differential diagnosis of hearing loss with air and bone Auditory Brainstem Response in small children has not been enough studied in Brazil. AIM: To compare air and bone Auditory Brainstem Response results in children under 2 months of age with normal hearing. STUDY DESIGN: clinical with transversal cohort. MATERIALS AND METHODS: 12 children who passed the hearing screening were evaluated using air and bone Auditory Brainstem Response. No contralateral masking was used in the bone conduction test. The responses were compared and analyzed by the McNemar test and repetitive measurements of the variance test. RESULTS: There were no statistic differences between air and bone

Estudo dos processos de mobilização, ativação e apoptose das células da medula óssea em modelo de morte encefálica em ratos

OpenAIRE

Laura Menegat

2016-01-01

INTRODUÇÃO: Estudos experimentais suportam a evidência de leucopenia persistente desencadeada pela morte encefálica (ME). OBJETIVO: Esse estudo teve como objetivo investigar o comportamento leucocitário na medula óssea e no sangue após a morte encefálica em ratos. MÉTODOS: A morte encefálica foi induzida através da inserção e insuflação rápida de um cateter no espaço intracraniano. Ratos falso-operados (FO) foram apenas trepanados. Decorridas seis horas, as células da medula óssea, coletadas ...

The comparison of Co-60 and 4MV photons matching dosimetry during half-beam technique

International Nuclear Information System (INIS)

Cakir, Aydin; Bilge, Hatice; Dadasbilge, Alpar; Kuecuecuek, Halil; Okutan, Murat; Merdan Fayda, Emre

2005-01-01

In this phantom study, we tried to compare matching dosimetry differences between half-blocking of Co-60 and asymmetric collimation of the 4MV photons during craniospinal irradiation. The dose distributions are compared and discussed. Firstly, some gaps with different sizes are left between cranial and spinal field borders. Secondly, the fields are overlapped in the same sizes. We irradiate the films located in water-equivalent solid phantoms with Co-60 and 4MV photon beams. This study indicates that the field placement errors in +/- 1mm are acceptable for both Co-60 and 4MV photon energies during craniospinal irradiation with half-beam block technique. Within these limits the dose variations are specified in +/- 5%. However, the setup errors that are more than 1mm are unacceptable for both asymmetric collimation of 4MV photon and half-blocking of Co-60

A histerectomia simples realizada no menacme e a densidade mineral óssea da mulher na pós-menopausa Pre-menopausal simple hysterectomy and post-menopausal female bone mineral density

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João Daniel Hobeika

2002-12-01

Full Text Available O objetivo foi avaliar a densidade mineral óssea de mulheres na pós-menopausa, com o antecedente de histerectomia com conservação ovariana bilateral, realizada no menacme, comparadas a um grupo de mulheres com menopausa natural, não histerectomizadas. Tratou-se de um estudo de corte transversal, com trinta mulheres na pós-menopausa e histerectomizadas no menacme e 102 mulheres menopausadas naturalmente, comparando-se as respectivas densitometrias ósseas, em nível do fêmur e coluna lombar. A análise mostrou que as variáveis idade e índice de massa corporal estavam fortemente associadas à densidade mineral óssea do fêmur e da coluna lombar, sendo o índice de massa corporal diretamente e a idade inversamente associados. Posteriormente, as trinta mulheres histerectomizadas, foram pareadas por idade e índice de massa corporal, com trinta mulheres não-histerectomizadas, não apresentando diferenças estatisticamente significantes da densidade mineral óssea. Esses resultados sugerem que a histerectomia com conservação ovariana bilateral, realizada em mulheres no menacme, não parece ocasionar redução adicional da massa óssea, quando avaliadas na pós-menopausa.This study aimed to evaluate the bone mineral density (BMD of post-menopausal women with previous pre-menopausal hysterectomy including bilateral ovarian conservation compared to a group of non-hysterectomized women with natural menopause. Data from a cross-sectional study of 30 pre-menopausally hysterectomized women evaluated in the post-menopause were compared with 102 naturally post-menopausal women, analyzing their respective bone densitometry, measuring the femoral and lumbar spinal BMD. Multiple regression analysis of the 132 women showed that age and body mass index (BMI were heavily associated with femoral and lumbar spinal BMD, BMI directly associated, and age inversely associated with BMD. In addition, 30 hysterectomized women were matched by age and BMI to the

46 CFR 189.60-1 - Application.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Application. 189.60-1 Section 189.60-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-1 Application...

Tensile bond strength of glass fiber posts luted with different cements Resistência à tração de pinos de fibra de vidro cimentados com diferentes materiais

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Gerson Bonfante

2007-06-01

Full Text Available Proper selection of the luting agent is fundamental to avoid failure due to lack of retention in post-retained crowns. The objective of this study was to investigate the tensile bond strength and failure mode of glass fiber posts luted with different cements. Glass fiber posts were luted in 40 mandibular premolars, divided into 4 groups (n = 10: Group 1 - resin-modified glass ionomer RelyX Luting; Group 2 - resin-modified glass ionomer Fuji Plus; Group 3 - resin cement RelyX ARC; Group 4 - resin cement Enforce. Specimens were assessed by tensile strength testing and light microscopy analysis for observation of failure mode. The tensile bond strength values of each group were compared by ANOVA and Tukey test. The significance level was set at 5%. The failure modes were described as percentages. The following tensile strength values were obtained: Group 1 - 247.6 N; Group 2 - 256.7 N; Group 3 - 502.1 N; Group 4 - 477.3 N. There was no statistically significant difference between Groups 1 and 2 or between Groups 3 and 4, yet the resin cements presented significantly higher tensile bond strength values than those presented by the glass ionomer cements. Group 1 displayed 70% of cohesive failures, whereas Groups 2, 3 and 4 exhibited 70% to 80% of adhesive failures at the dentin-cement interface. We concluded that resin cements and glass ionomer cements are able to provide clinically sufficient retention of glass fiber posts, and that glass ionomer cements may be especially indicated when the application of adhesive techniques is difficult.A seleção adequada do agente cimentante é essencial para evitar falhas por perda de retenção em coroas retidas por núcleos. O objetivo deste estudo foi investigar a resistência à tração e o tipo de falha de pinos de fibra de vidro cimentados com diferentes materiais. Cimentaram-se pinos de fibra de vidro em 40 pré-molares inferiores, divididos em 4 grupos (n = 10: Grupo 1 - ionômero de vidro modificado

Effect of He pressure on the superconducting transition temperatures of Na2CsC60 and (NH3)4Na2CsC60

International Nuclear Information System (INIS)

Schirber, J.E.; Bayless, W.R.; Rosseinsky, M.J.; Zhou, O.; Fleming, R.M.; Murphy, D.; Fischer, J.E.

1994-01-01

The Na based mixed alkali doped C 60 superconductors show anomalous behavior with respect to the ''universal'' superconducting transition temperature T c vs lattice constant a 0 relation followed by most of the fcc A 3 C 60 superconductors. We have measured dt c /dP for Na 2 CsC 60 and (NH 3 ) 4 Na 2 CsC 60 using solid He as the pressure medium to ∼ 6 kbar finding dT c /dP equal to -0.8±(0.01) K/kbar and -1.0(±0.1)K/kbar for Na 2 CsC 60 and Na 2 (NH 3 ) 4 C 60 respectively. Our value for Na 2 CsC 60 differs markedly from that obtained by Mizuki et al of about -1.3 K/kbar. However, using N 2 or Ar, we obtain values for dT c /dP in substantial agreement with Mizuki et al who used fluorinert to generate their pressure. This work emphasizes the need for compressibility measurements with the same pressure medium in the appropriate temperature range so that meaningful comparisons can be made between various pressure measurements and models which are based on lattic spacing

41 CFR 60-1.1 - Purpose and application.

Science.gov (United States)

2010-07-01

... obligations under the equal opportunity clause regardless of whether or not his contract contains a “Disputes... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Purpose and application. 60-1.1 Section 60-1.1 Public Contracts and Property Management Other Provisions Relating to Public...

miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells.

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Emilio Satoshi Hara

Full Text Available Dental pulp cells (DPCs are known to be enriched in stem/progenitor cells but not well characterized yet. Small non-coding microRNAs (miRNAs have been identified to control protein translation, mRNA stability and transcription, and have been reported to play important roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In order to characterize dental pulp stem/progenitor cells and its mechanism of differentiation, we herein sorted stem-cell-enriched side population (SP cells from human DPCs and periodontal ligament cells (PDLCs, and performed a locked nucleic acid (LNA-based miRNA array. As a result, miR-720 was highly expressed in the differentiated main population (MP cells compared to that in SP cells. In silico analysis and a reporter assay showed that miR-720 targets the stem cell marker NANOG, indicating that miR-720 could promote differentiation of dental pulp stem/progenitor cells by repressing NANOG. Indeed, gain-and loss-of-function analyses showed that miR-720 controls NANOG transcript and protein levels. Moreover, transfection of miR-720 significantly decreased the number of cells positive for the early stem cell marker SSEA-4. Concomitantly, mRNA levels of DNA methyltransferases (DNMTs, which are known to play crucial factors during stem cell differentiation, were also increased by miR-720 through unknown mechanism. Finally, miR-720 decreased DPC proliferation as determined by immunocytochemical analysis against ki-67, and promoted odontogenic differentiation as demonstrated by alizarin red staining, as well as alkaline phosphatase and osteopontin mRNA levels. Our findings identify miR-720 as a novel miRNA regulating the differentiation of DPCs.

Polímero derivado de mamona acrescido de cálcio, associado ou não à medula óssea autógena na reparação de falhas ósseas Castor oil plant polymer and calcium associated or not to autogenous bone marrow in bone gaps repair

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Ricardo Junqueira Del Carlo

2003-12-01

Full Text Available Foram avaliados o processo de reparação tecidual e o comportamento do implante de polímero vegetal extraído do óleo de mamona acrescido de 40% de carbonato de sódio, associado ou não à impregnação com medula óssea autógena, em falhas ósseas experimentais em rádios de 30 coelhos e em sítio heterotópico em seis animais. Em quinze coelhos, a falha óssea no rádio direito foi preenchida por cilindros de polímero de mamona (grupo P com dimensão semelhante à da falha; a falha no rádio direito dos outros coelhos recebeu aspirado de medula óssea autógena junto com o implante (grupo M. A falha óssea no membro esquerdo de cada coelho não recebeu nenhum tratamento e serviu como controle. Os seis coelhos restantes receberam seis implantes no músculo reto abdominal (sítio heterotópico, sendo que, em três animais, os implantes estavam embebidos em aspirado de medula óssea autógena. No local do implante, em ambos os grupos, foi observado aumento da radiopacidade, sem desvio de eixo ósseo ou reabsorção das extremidades ósseas receptoras. O grupo P apresentou áreas irregulares de calcificação na região periférica e sobre o polímero e o M apresentou um padrão de radiopacidade mais intenso, regular e precoce em relação ao P. Na avaliação histológica, em ambos os grupos, foi observada formação de tecido ósseo imaturo com tendência à organização, brotos isolados de formação de osso novo sobre o polímero em seus poros superficiais e nos poros internos que se comunicavam. Quando foi associado à medula, o implante permitiu a ocorrência de osteocondução e osteogênese progressiva; houve migração de capilares, tecidos perivasculares e células osteoprogenitoras entre os poros, com tecido fibrovascular invadindo a superfície do implante; a incorporação dos implantes deu-se de maneira lenta e estava incompleta até as nove semanas do estudo; o implante foi biocompatível no período avaliado. Em s

46 CFR 193.60-1 - Application.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Application. 193.60-1 Section 193.60-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS FIRE PROTECTION EQUIPMENT Fire Axes § 193.60-1 Application. (a) The provisions of this subpart shall apply to all vessels...

Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue.

Science.gov (United States)

Qadan, Maha A; Piuzzi, Nicolas S; Boehm, Cynthia; Bova, Wesley; Moos, Malcolm; Midura, Ronald J; Hascall, Vincent C; Malcuit, Christopher; Muschler, George F

2018-03-01

Connective tissue progenitors (CTPs) embody the heterogeneous stem and progenitor cell populations present in native tissue. CTPs are essential to the formation and remodeling of connective tissue and represent key targets for tissue-engineering and cell-based therapies. To better understand and characterize CTPs, we aimed to compare the (i) concentration and prevalence, (ii) early in vitro biological behavior and (iii) expression of surface-markers and transcription factors among cells derived from marrow space (MS), trabecular surface (TS), and adipose tissues (AT). Cancellous-bone and subcutaneous-adipose tissues were collected from 8 patients. Cells were isolated and cultured. Colony formation was assayed using Colonyze software based on ASTM standards. Cell concentration ([Cell]), CTP concentration ([CTP]) and CTP prevalence (P CTP ) were determined. Attributes of culture-expanded cells were compared based on (i) effective proliferation rate and (ii) expression of surface-markers CD73, CD90, CD105, SSEA-4, SSEA-3, SSEA-1/CD15, Cripto-1, E-Cadherin/CD324, Ep-CAM/CD326, CD146, hyaluronan and transcription factors Oct3/4, Sox-2 and Nanog using flow cytometry. Mean [Cell], [CTP] and P CTP were significantly different between MS and TS samples (P = 0.03, P = 0.008 and P= 0.0003), respectively. AT-derived cells generated the highest mean total cell yield at day 6 of culture-4-fold greater than TS and more than 40-fold greater than MS per million cells plated. TS colonies grew with higher mean density than MS colonies (290 ± 11 versus 150 ± 11 cell per mm 2 ; P = 0.0002). Expression of classical-mesenchymal stromal cell (MSC) markers was consistently recorded (>95%) from all tissue sources, whereas all the other markers were highly variable. The prevalence and biological potential of CTPs are different between patients and tissue sources and lack variation in classical MSC markers. Other markers are more likely to discriminate differences

Considerazioni sul problema dei rigassificatori tra aggregazione del consenso e conflitto ambientale

OpenAIRE

Lodoli, Flavio

2011-01-01

2009/2010 Considerazioni sul problema dei Rigassificatori tra Aggregazione del Consenso e Conflitto Ambientale 1. Il nostro Paese, per scelta politica e per una sostanziale limitata capacità di innovazione del mondo industriale, ha una quota attuale di produzione di energia da fonti rinnovabili poco significativa nonostante gli investimenti, i contributi e le agevolazioni fiscali che dovrebbero incentivare opportunamente ad esempio, l’utilizzo dell’energia solare che rappresenta una...

Uso do feromônio sexual sintético para captura de machos da traça-das-crucíferas

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MICHEREFF MIRIAN FERNANDES FURTADO

2000-01-01

Full Text Available Este trabalho teve como objetivo estudar a atração dos machos da traça-das-crucíferas (Plutella xylostella L., Lepidoptera: Yponomeutidae por diferentes formulações do feromônio sexual sintético. Os tratamentos consistiram em: 1 formulação comercial; 2 Z11,16:Ald; 3 Z11,16:Ac; 4 mistura 7:3 de 2+3; 5 mistura 5:5 de 2+3; 6 septos de borracha com hexano (testemunha; e 7 cinco fêmeas virgens. A formulação comercial do feromônio sexual sintético propiciou maior captura de machos, não diferindo significativamente de fêmeas virgens e da mistura 5:5 utilizadas como isca. Os componentes isoladamente foram pouco atrativos aos machos. Foram testados cinco modelos de armadilhas. A armadilha Pherocon 1 CP (33,2 machos/armadilha/noite foi a mais eficiente na captura de machos, seguida pelas armadilhas Cilíndrica Aberta, Cilíndrica Fechada, Delta e Redonda Aberta. Três alturas de instalação da armadilha foram avaliadas. Ocorreu significativamente maior captura de machos na altura do ápice das plantas (30 cm do solo. As alturas de 5 e 60 cm não diferiram entre si.

HA117 endows HL60 cells with a stem-like signature by inhibiting the degradation of DNMT1 via its ability to down-regulate expression of the GGL domain of RGS6.

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Shuangshuang Li

Full Text Available All-trans retinoic acid (ATRA induces complete remission in almost all patients with acute promyelocytic leukemia (APL via its ability to induce the in vivo differentiation of APL blasts. However, prolonged ATRA treatment can result in drug resistance. In previous studies, we generated a multi-drug-resistant HL60/ATRA cell line and found it to contain a new drug resistance-related gene segment, HA117. In this study, we demonstrate that ATRA induces multi-drug-resistant subpopulations of HL60 cells with a putative stem-like signature by up-regulating the expression of the new gene segment HA117. Western blot analysis and quantitative real-time PCR demonstrated that HA117 causes alternative splicing of regulator of G-protein signaling 6 (RGS6 and down-regulation of the expression of the GGL domain of RGS6, which plays an important role in DNA methyltransferase 1 (DNMT1 degradation. Moreover, DNMT1 expression was increased in multi-drug resistance HL60/ATRA cells. Knockdown of HA117 restored expression of the GGL domain and blocked DNMT1 expression. Moreover, resistant cells displayed a putative stem-like signature with increased expression of cancer steam cell markers CD133 and CD123. The stem cell marker, Nanog, was significantly up-regulated. In conclusion, our study shows that HA117 potentially promotes the stem-like signature of the HL60/ATRA cell line by inhibiting by the ubiquitination and degradation of DNMT1 and by down-regulating the expression of the GGL domain of RGS6. These results throw light on the cellular events associated with the ATRA-induced multi-drug resistance phenotype in acute leukemia.

Human amniotic fluid stem cells (hAFSCs expressing p21 and cyclin D1 genes retain excellent viability after freezing with (dimethyl sulfoxide DMSO

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Shiva Gholizadeh-Ghaleh Aziz

2018-04-01

Full Text Available Human amniotic fluid stem cells (hAFSCs have features intermediate between embryonic and adult SCs, can differentiate into lineages of all three germ layers, and do not develop into tumors in vivo. Moreover, hAFSCs can be easily obtained in routine procedures and there is no ethical or legal limitations regarding their use for clinical and experimental applications. The aim of this study was to assess the effect of slow freezing/thawing and two different concentrations of DMSO (10% DMSO + 90% fetal bovine serum [FBS] and 5% DMSO + 95% FBS on the survival of hAFSCs. hAFSCs were obtained from 5 pregnant women during amniocentesis at 16–22 weeks of gestation. The expression of pluripotency markers (Octamer-binding transcription factor 4 [Oct4] and NANOG by reverse transcription polymerase chain reaction and cell surface markers (cluster of differentiation [CD31], CD44, CD45, and CD90 by flow cytometry was analyzed before and after the slow-freezing. Cell viability was assessed by trypan blue exclusion or MTT assay. Quantitative mRNA expression of Oct4, NANOG, cyclin D1 and p21 was determined by real-time PCR before and after the slow-freezing. Pluripotency of hAFSCs was confirmed by NANOG and POU5F1 (Oct4 gene expression before and after slow-freezing. All hAFSC cultures were positive for CD44 and CD90. A higher viability of hAFSCs was observed after freezing with 90% FBS + 10% DMSO. There was increased expression of NANOG and decreased expression of POU5F1 gene after freezing, compared to control cells (before freezing. DMSO and the process of freezing did not significantly change the expression of p21 and cyclin D1 genes in hAFSCs. Overall, our results indicate the applicability of slow-freezing and DMSO in cryopreservation of SCs.

Estudo prospectivo comparativo entre pseudoartrose e fusão óssea na estenose de canal lombar Prospective-comparative study between pseudarthrosis and bone fusion in lumbar stenosis

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Luciano Miller Reis Rodrigues

2011-01-01

Full Text Available OBJETIVO: Estudo prospectivo comparativo entre pseudoartrose e fusão óssea na estenose de canal lombar MÉTODO: 38 pacientes operados de estenose de canal lombar e submetidos à artrodese avaliados por meio de questionários (escala visual analógica - VAS e questionário de incapacidade Rolland Morris. Foram solicitadas radiografias para avaliação da fusão lombar. RESULTADOS: Foi observada uma efetiva melhora entre o momento pré-operatório e após um ano em relação ao VAS, tanto no grupo que obteve fusão óssea como no grupo com pseudartrose. Em relação ao questionário Rolland Morris houve uma tendência de melhora no grupo com fusão óssea e uma significante melhora no grupo com pseudoartrose. CONCLUSÃO: Não houve diferença entre os grupos (fusão óssea e pseudoartrose em relação à dor e incapacidade. Nível de Evidência: Nível II, estudo prospectivo longitudinal.OBJECTIVE: Prospective-comparative study between pseudarthrosis and bone fusion in lumbar stenosis METHODS: 38 patients operated on for lumbar spinal stenosis and submitted to arthrodesis were evaluated by the Visual Analogue Scale (VAS and Roland Morris Disability Questionnaire. Radiographs were requested to evaluate spinal fusion. RESULTS: An effective improvement was observed between the preoperative period and one year after the operation, in relation to VAS, both in the group that received a bone fusion and in the group with pseudarthrosis. With regard to the Roland Morris questionnaire, there was a tendency towards an improvement in the bone fusion group and a significant improvement in the pseudarthrosis group. CONCLUSION: There was no difference between the groups (bone fusion and pseudarthrosis in relation to pain and disability. . Level of Evidence: Level II, longitudinal prospective study.

The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal.

Science.gov (United States)

Organista-Nava, Jorge; Gómez-Gómez, Yazmín; Ocadiz-Delgado, Rodolfo; García-Villa, Enrique; Bonilla-Delgado, José; Lagunas-Martínez, Alfredo; Tapia, Jesús Santa-Olalla; Lambert, Paul F; García-Carrancá, Alejandro; Gariglio, Patricio

2016-12-01

Oct3/4 is a transcription factor involved in maintenance of the pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17β-estradiol (E 2 ) are key factors in cervical carcinogenesis. In the present study, we aimed to investigate the effect of the HPV16 E7 oncoprotein and E 2 on the expression pattern of Oct3/4, Sox2, Nanog and Fgf4. We also determined whether the E7 oncoprotein is associated with cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4 were upregulated by the E7 oncoprotein in vivo and in vitro and implicate E 2 in the upregulation of these factors in vivo. We also demonstrated that E7 is involved in cell self-renewal, suggesting that the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. Copyright © 2016 Elsevier Inc. All rights reserved.

Simultaneous spectral and photometric observations of the beat Cepheid U TrA

International Nuclear Information System (INIS)

Niva, G.D.; Schmidt, E.G.

1981-01-01

It was suggested that U TrA was a Cepheid with a modulated light curve. Further photometric and radial-velocity observations have confirmed this behaviour. Unfortunately, the radial velocities are too few in number and too scattered to allow a detailed analysis. This paper presents further photometric and spectroscopic observations of U TrA. The original intent was to obtain enough simultaneous observations to perform a Wesselink analysis similar to the one made for another beat Cepheid, TU Cas. Unfortunately, this has not been possible. However, the data obtained are of high quality and are clearly useful in studies of the modal content and period stability of the star. (author)

Assessment of exposure to traffic pollution using the ExTra index: study of validation

International Nuclear Information System (INIS)

Reungoat, Patrice; Chiron, Mireille; Gauvin, Stephanie; Le Moullec, Y.; Momas, Isabelle

2003-01-01

The ExTra index, produced by the French Scientific Center for Building Physics, evaluates ambient concentrations of transport-related pollutants in front of the work and living places of urban dwellers. This study contributes to the validation of the ExTra index by carrying out measurements in four French cities. It compares nitrogen oxide concentrations (NO x ) measured over 6 weeks with passive samplers, and NO x calculated concentrations using the ExTra index. The study takes into account traffic density, topographical parameters (building height, road, and pavement width), weather conditions (wind direction and strength), and background pollution levels. The model was tested at 100 street canyons sites. There were highly significant correlations (0.90 in Grenoble, 0.95 in Nice, 0.89 in Paris, and 0.89 in Toulouse) and good intraclass correlation coefficients (0.75 in Grenoble, 0.91 in Nice, 0.89 in Paris, and 0.86 in Toulouse) between the two series of values. These results suggest that if the ExTra index were to be applied to all the different residences and workplaces of a subject throughout his (her) life, it could be a useful epidemiological tool for studying the long-term health effects of transport-related emissions and for reconstructing individual exposure to such pollution in order to avoid misclassification

Study on preparation and effect of oligoβ-glucan and oligochitosan on immune stimulation white patches in the internal organs disease on Tra catfish (Pangasianodon hypophthalmus)

International Nuclear Information System (INIS)

Nguyen Ngoc Duy; Dang Van Phu; Nguyen Thi Kim Lan; Nguyen Quoc Hien; Pham Duy Hai

2015-01-01

Oligoβ-glucan and oligochitosan were prepared by gamma Co-60 irradiation of β-glucan/H_2O_2 and chitosan/H_2O_2 solution. The efficiency of the degradation process was determined by gel permeation chromatography (GPC) method. Results showed that the Mw decreased with increasing concentration of H_2O_2 and doses. For oligoβ-glucan, Mw reduced from 56.7 kDa to 7.1 kDa when β-glucan 10%/H_2O_2 1% solution was irradiated at 14 kGy. For oligochitosan, Mw reduced from 45.5 kDa to 5.0 kDa when chitosan 5%/H_2O_2 0.5% solution was irradiated at 21 kGy. Tra catfish (Pangasianodon hypophthalmus) was fed with oligoβ-glucan and oligochitosan in various concentrations of 0, 50, 100, and 200 mg/kg feed for 45 days and then was challenged with Edwardsiella ictaluri bacteria to investigate immune stimulation effect against white patches in the internal organs disease. The results indicated that oligoβ-glucan and oligochitosan exhibited good immune stimulation effect with optimum concentration of 100 mg/kg feed. Survival rate of Tra catfishes fed with oligochitosan and oligoβ-glucan is 47.62 ± 1.96% and 46.67 ± 2.58%, respectively. In addition, the mixture of oligochitosan 50 mg/kg + oligo?-glucan 50 mg/kg showed the highest survival rate (62.22 ± 1.96%). (author)

The system Ba(H2PO4)2-Sr(H2PO4)2-H3PO4(30%)-H2O at 25, 40 and 60 deg C

International Nuclear Information System (INIS)

Taranenko, N.P.; Serebrennikova, G.M.; Stepin, B.D.; Oboznenko, Yu.V.

1982-01-01

The system Ba(H 2 PO 4 ) 2 -Sr(H 2 PO 4 ) 2 -H 3 PO 4 (30%)-H 2 O (25 deg C) belongs to eutonic type systems. Solubility isotherms of salt components at 40 and 60 deg C are calculated. Polytherms (25-60 deg C) of solubility of monosubstituted barium and strontium phosphates in 30-60% H 3 PO 4 are obtained. The value of cocrystallization coefficient of Sr 2 + and Ba(H 2 PO 4 ) 2 Dsub(Sr)=0.042+-0.005 remains stable in the temperature range of 25-60 deg C and concentrations 30-60% phosphoric acid at initial content [Sr 2 + ]=1x10 - 2 mass%

Cartografar é traçar um plano comum

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Virgínia Kastrup

2013-08-01

Full Text Available A pesquisa de campo sobre produção da subjetividade enfrenta o problema de construir conhecimento envolvendo pesquisadores e pesquisados, com territórios e semióticas singulares. Surgem questões relativas ao protagonismo dos participantes e a como traçar com eles um plano comum, garantindo o caráter participativo da pesquisa. No contexto do método da cartografia, o artigo tem como objetivo tratar do tema do comum num duplo aspecto. Num primeiro, discute o acesso ao plano comum. Com base em Gilles Deleuze e Felix Guattari, tal plano não é dito homogêneo nem reúne atores que manteriam entre si relações de identidade, mas opera comunicação entre singularidades, sendo pré-individual e coletivo. Num segundo aspecto, aponta que, enquanto pesquisa-intervenção, a cartografia se compromete com a criação de um mundo comum e heterogêneo. O artigo mostra que o traçado do comum tem como diretriz metodológica a tranversalidade e examina os procedimentos de participação, inclusão e tradução.

The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal

Energy Technology Data Exchange (ETDEWEB)

Organista-Nava, Jorge; Gómez-Gómez, Yazmín [Programa de Doctorado en Ciencias Biomédicas, Instituto de Fisiología Celular (IFC), Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, México (Mexico); Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07360, México (Mexico); Ocadiz-Delgado, Rodolfo; García-Villa, Enrique [Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07360, México (Mexico); Bonilla-Delgado, José [Unidad de Investigación, Hospital Juárez de México, Ciudad de México 07760, México (Mexico); Lagunas-Martínez, Alfredo [División de Biología Molecular de Patógenos, CISEI, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México (Mexico); and others

2016-12-15

Oct3/4 is a transcription factor involved in maintenance of the pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17β-estradiol (E{sub 2}) are key factors in cervical carcinogenesis. In the present study, we aimed to investigate the effect of the HPV16 E7 oncoprotein and E{sub 2} on the expression pattern of Oct3/4, Sox2, Nanog and Fgf4. We also determined whether the E7 oncoprotein is associated with cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4 were upregulated by the E7 oncoprotein in vivo and in vitro and implicate E{sub 2} in the upregulation of these factors in vivo. We also demonstrated that E7 is involved in cell self-renewal, suggesting that the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. -- Graphical abstract: The HPV16 E7 oncoprotein and 17β-estradiol are involved in the upregulation of Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal ability of cancer stem cells in cervical cancer. - Highlights: •The HPV16 E7 oncoprotein enhances cellular proliferation and dedifferentiation. •The E7 oncoprotein induces stemness-related genes expression in vivo and in vitro. •The 17β-estradiol induces stemness-related genes expression in vivo. •The HPV16 E7 oncoprotein is involved in the cell self-renewal of cancer cells.

The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal

International Nuclear Information System (INIS)

Organista-Nava, Jorge; Gómez-Gómez, Yazmín; Ocadiz-Delgado, Rodolfo; García-Villa, Enrique; Bonilla-Delgado, José; Lagunas-Martínez, Alfredo

2016-01-01

Oct3/4 is a transcription factor involved in maintenance of the pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17β-estradiol (E 2 ) are key factors in cervical carcinogenesis. In the present study, we aimed to investigate the effect of the HPV16 E7 oncoprotein and E 2 on the expression pattern of Oct3/4, Sox2, Nanog and Fgf4. We also determined whether the E7 oncoprotein is associated with cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4 were upregulated by the E7 oncoprotein in vivo and in vitro and implicate E 2 in the upregulation of these factors in vivo. We also demonstrated that E7 is involved in cell self-renewal, suggesting that the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. -- Graphical abstract: The HPV16 E7 oncoprotein and 17β-estradiol are involved in the upregulation of Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal ability of cancer stem cells in cervical cancer. - Highlights: •The HPV16 E7 oncoprotein enhances cellular proliferation and dedifferentiation. •The E7 oncoprotein induces stemness-related genes expression in vivo and in vitro. •The 17β-estradiol induces stemness-related genes expression in vivo. •The HPV16 E7 oncoprotein is involved in the cell self-renewal of cancer cells.

Impacto dos nutrientes na saúde óssea: novas tendências Nutrients impact on bone health: new trends

Directory of Open Access Journals (Sweden)

Glaucia Queiroz Morais

2007-07-01

Full Text Available A nutrição é fator importante modificável no desenvolvimento/manutenção da massa óssea (MO e prevenção da osteoporose. Esta é uma doença caracterizada por decréscimo na massa esquelética e elevação da suscetibilidade a fraturas. O desenvolvimento do pico de massa óssea (PMO é ainda o maior determinante dessa condição. Por isso, alterações com a idade sinalizam a importância da manutenção de nutrição adequada durante o desenvolvimento desse pico. Indivíduos que atingiram elevado PMO terão, na idade adulta, baixo risco de desenvolver doenças osteometabólicas com o envelhecimento. Os efeitos dos nutrientes sobre as estruturas esqueléticas são intensos e amplos; alguns, há anos, são consistentemente relacionados com a saúde óssea, como o cálcio, fósforo e vitamina D. Entretanto, outros nutrientes vêm sendo sugeridos como essenciais para o metabolismo ósseo normal: proteínas, lipídeos, potássio, vitaminas K e A e cloreto de sódio. A influência das interações entre tais fatores nutricionais, meio ambiente, estilo de vida e hereditariedade auxiliarão na compreensão da complexidade do desenvolvimento da osteoporose e subseqüentes fraturas. Em adição, déficit energético pode acarretar redução da MO, em face dos distúrbios endócrino-metabólicos desencadeados pela privação alimentar. Esta revisão objetiva elucidar o impacto dos nutrientes e ingestão calórica sobre o osso, em diferentes estágios da vida, descrevendo possíveis interações entre nutrientes e como estas poderiam afetar a homeostase óssea e mineral.Nutrition is an important factor in bone mass development/maintenance and in osteoporosis prevention. This disease is characterized by decreased skeletal mass and increased susceptibility to fractures. The greatest determinant of this condition is the peak bone mass (PBM. Hence, alterations over time indicate the importance of maintaining adequate nutrition during the development of

Risk factors for osteoporotic fractures and low bone density in pre and postmenopausal women Factores de riesgo para fractura por osteoporosis y baja densidad ósea en mujeres en la pre y post menopausia Fatores de risco para fratura por osteoporose e baixa densidade óssea em mulheres na pré e pós-menopausa

Directory of Open Access Journals (Sweden)

Marcelo M Pinheiro

2010-06-01

com 4.332 mulheres acima de 40 anos de idade provenientes de atendimento primário de saúde na área metropolitana da Grande São Paulo, SP, entre 2004 e 2007. Dados antropométricos e ginecológicos e relativos a hábitos de vida, fratura prévia, antecedentes pessoais, ingestão alimentar e atividade física foram avaliados por meio de entrevista individual e quantitativa. Fratura por baixo impacto foi definida como decorrente de queda da própria altura ou menos em indivíduos com mais de 50 anos de idade. Modelos de regressão multivariada e logística analisaram, respectivamente, a densidade óssea e a fratura por osteoporose como variáveis dependentes e todas as outras como independentes. O nível de significância estatística estabelecido foi p < 0,05. RESULTADOS: A prevalência de osteoporose e de fraturas por fragilidade óssea foi de 33% e 11,5%, respectivamente. Os principais fatores de risco associados com baixa densidade óssea foram idade (OR = 1,07; IC 95%: 1,06;1,08, menopausa (OR = 2,16; IC 95%: 1,49;3,14, fratura prévia (OR = 2,62; IC 95%: 2,08;3,29 e tabagismo atual (OR = 1,45; IC 95%: 1,13;1,85. Por outro lado, elevado IMC (OR = 0,88; IC 95%: 0,86;0,89, atividade física regular (OR = 0,78; IC 95%: 0,65;0,94 e terapia hormonal atual (OR = 0,43; IC 95%: 0,33;0,56 desempenharam papel protetor. Os fatores de risco significativamente relacionados com fratura por osteoporose foram idade (OR = 1,05; IC 95%: 1,04;1,06, menopausa (OR = 4,12; IC 95%: 1,79;9,48, história familiar de fratura de quadril (OR = 3,59; IC 95%: 2,88;4,47 e baixa densidade óssea (OR = 2,28; IC 95%: 1,85;2,82. CONCLUSÕES: Idade avançada, menopausa, fratura prévia por baixo impacto e tabagismo atual são os principais fatores de risco associados com baixa densidade óssea, a qual se associa com as fraturas por fragilidade óssea. O uso clínico desses parâmetros para identificar mulheres de maior risco para fraturas pode ser uma estratégia interessante para melhorar a

Protein profile of basal prostate epithelial progenitor cells--stage-specific embryonal antigen 4 expressing cells have enhanced regenerative potential in vivo.

Science.gov (United States)

Höfner, Thomas; Klein, Corinna; Eisen, Christian; Rigo-Watermeier, Teresa; Haferkamp, Axel; Sprick, Martin R

2016-04-01

The long-term propagation of basal prostate progenitor cells ex vivo has been very difficult in the past. The development of novel methods to expand prostate progenitor cells in vitro allows determining their cell surface phenotype in greater detail. Mouse (Lin(-)Sca-1(+) CD49f(+) Trop2(high)-phenotype) and human (Lin(-) CD49f(+) TROP2(high)) basal prostate progenitor cells were expanded in vitro. Human and mouse cells were screened using 242 anti-human or 176 antimouse monoclonal antibodies recognizing the cell surface protein profile. Quantitative expression was evaluated at the single-cell level using flow cytometry. Differentially expressed cell surface proteins were evaluated in conjunction with the known CD49f(+)/TROP2(high) phenotype of basal prostate progenitor cells and characterized by in vivo sandwich-transplantation experiments using nude mice. The phenotype of basal prostate progenitor cells was determined as CD9(+)/CD24(+)/CD29(+)/CD44(+)/CD47(+)/CD49f(+)/CD104(+)/CD147(+)/CD326(+)/Trop2(high) of mouse as well as human origin. Our analysis revealed several proteins, such as CD13, Syndecan-1 and stage-specific embryonal antigens (SSEAs), as being differentially expressed on murine and human CD49f(+) TROP2(+) basal prostate progenitor cells. Transplantation experiments suggest that CD49f(+) TROP2(high) SSEA-4(high) human prostate basal progenitor cells to be more potent to regenerate prostate tubules in vivo as compared with CD49f(+) TROP2(high) or CD49f(+) TROP2(high) SSEA-4(low) cells. Determination of the cell surface protein profile of functionally defined murine and human basal prostate progenitor cells reveals differentially expressed proteins that may change the potency and regenerative function of epithelial progenitor cells within the prostate. SSEA-4 is a candidate cell surface marker that putatively enables a more accurate identification of the basal PESC lineage. © 2016 The Authors. Journal of Cellular and Molecular Medicine published by

Viabilidade da mensuração de marcadores de remodelação óssea em mulheres com lúpus eritematoso sistêmico

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Jaroslaw Bogaczewicz

2015-04-01

Full Text Available Objetivo: Investigar a viabilidade dos marcadores de remodelação óssea (MRO na avaliação do metabolismo ósseo em pacientes com lúpus eritematoso sistêmico (LES, de acordo com as diretrizes da International Osteoporosis Foundation e da International Federation of Clinical Chemistry and Laboratory Medicine. Métodos: O estudo incluiu 43 pacientes do sexo feminino com LES. Foram medidos os níveis séricos de propeptídeo N-terminal do procolágeno tipo I (PINP, telopeptídeo C-terminal do colágeno tipo I (CTX, osteocalcina, HPT, 25(OHD, anticorpos anticardiolipina, antidsDNA e antinucleossomo. Resultados: Os níveis de PINP e CTX estavam elevados em pacientes com LES com idade > 45, em comparação com aqueles com idade 45 (p < 0,001. Não houve diferença estatisticamente significativa nos níveis de PINP, osteocalcina ou CTX com relação à estação do ano, nem em todo o grupo de pacientes com LES, nem naqueles com mais ou menos de 45 anos. O uso prévio de glucocorticoides não esteve associado a diferenças nos MRO. Conclusões: O aumento nos MRO no LES parece refletir predominantemente o padrão de remodelação óssea relacionado com a idade. Pode-se esperar que o PINP aumentado seja o desfecho mais comumente encontrado entre os MRO. É necessário incluir melhores diagnósticos de distúrbios ósseos com MRO, feitos de acordo com as normas internacionais de referência, na abordagem de pacientes com LES, além de avaliar a densidade mineral óssea.

Giorgio Perlasca, 'Giusto tra le nazioni' e 'eroe italiano'

NARCIS (Netherlands)

Jansen, M.M.

2008-01-01

Giorgio Perlasca che, fingendosi console spagnolo, nel 1944 salvò più di cinquemila ebrei ungheresi a Budapest, è stato proclamato “Giusto tra le Nazioni” a Yad Vashem nel 1989, dopo di che è seguito il suo riconoscimento all’estero ed in Italia come “uomo giusto” e come “eroe italiano”. Dopo il

Análise quantitativa das células de Langerhans em mucosa bucal de pacientes submetidos ao transplante de medula óssea alogênico com doença enxerto contra hospedeiro crônica

OpenAIRE

Érika Sinara Lenharo Orti-Raduan

2007-01-01

A doença enxerto contra hospedeiro é uma complicação comum nos pacientes submetidos ao transplante de medula óssea alogênico. Com o objetivo de contribuir para o esclarecimento da participação das células de Langerhans na doença enxerto contra hospedeiro crônica (GVHDc) quando de sua ocorrência na mucosa bucal, foram analisados 40 pacientes oncohematológicos e hematológicos submetidos ao transplante de medula óssea alogênico no Hospital Amaral Carvalho, Jaú - SP. Cortes microscópicos de 3µm d...

Electronic structure evolution and energy level alignment at C60/4,4'-cyclohexylidenebis[N,N-bis(4-methylphenyl) benzenamine]/MoOx/indium tin oxide interfaces

Science.gov (United States)

Liu, Xiaoliang; Yi, Shijuan; Wang, Chenggong; Wang, Congcong; Gao, Yongli

2014-04-01

The electronic structure evolution and energy level alignment have been investigated at interfaces comprising fullerene (C60)/4,4'-cyclohexylidenebis[N,N-bis(4-methylphenyl) benzenamine] (TAPC)/ molybdenum oxide (MoOx)/ indium tin oxide with ultraviolet photoemission spectroscopy and inverse photoemission spectroscopy. With deposition of TAPC upon MoOx, a dipole of 1.58 eV was formed at the TAPC/MoOx interface due to electron transfer from TAPC to MoOx. The highest occupied molecular orbital (HOMO) onset of TAPC was pinned closed to the Fermi level, leading to a p-doped region and thus increasing the carrier concentration at the very interface. The downward band bending and the resulting built-in field in TAPC were favorable for the hole transfer toward the TAPC/MoOx interface. The rigid downward shift of energy levels of TAPC indicated no significant interface chemistry at the interface. With subsequent deposition of C60 on TAPC, a dipole of 0.27 eV was observed at the C60/TAPC heterojunction due to the electron transfer from TAPC to C60. This led to a drop of the HOMO of TAPC near the C60/TAPC interface, and hence further enhanced the band bending in TAPC. The band bending behavior was also observed in C60, similarly creating a built-in field in C60 film and improving the electron transfer away from the C60/TAPC interface. It can be deduced from the interface analysis that a promising maximum open circuit voltage of 1.5 eV is achievable in C60/TAPC-based organic photovoltaic cells.

Laminin binding protein, 34/67 laminin receptor, carries stage-specific embryonic antigen-4 epitope defined by monoclonal antibody Raft.2

International Nuclear Information System (INIS)

Katagiri, Yohko U.; Kiyokawa, Nobutaka; Nakamura, Kyoko; Takenouchi, Hisami; Taguchi, Tomoko; Okita, Hajime; Umezawa, Akihiro; Fujimoto, Junichiro

2005-01-01

We previously produced monoclonal antibodies against the detergent-insoluble microdomain, i.e., the raft microdomain, of the human renal cancer cell line ACHN. Raft.2, one of these monoclonal antibodies, recognizes sialosyl globopentaosylceramide, which has the stage-specific embryonic antigen (SSEA)-4 epitope. Although the mouse embryonal carcinoma (EC) cell line F9 does not express SSEA-4, some F9 cells stained with Raft.2. Western analysis and matrix-assisted laser desorption ionization-time of flight mass spectrometry identified the Raft.2 binding molecule as laminin binding protein (LBP), i.e., 34/67 laminin receptor. Weak acid treatment or digestion with Clostridium perfringens sialidase reduced Raft.2 binding to LBP on nitrocellulose sheets and [ 14 C]galactose was incorporated into LBP, indicating LBP to have a sialylated carbohydrate moiety. Subcellular localization analysis by sucrose density-gradient centrifugation and examination by confocal microscopy revealed LBP to be localized on the outer surface of the plasma membrane. An SSEA-4-positive human EC cell line, NCR-G3 cells, also expressed Raft.2-binding LBP

41 CFR 60-1.5 - Exemptions.

Science.gov (United States)

2010-07-01

... society, or if the curriculum of such school, college, university, or other educational institution or... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Exemptions. 60-1.5 Section 60-1.5 Public Contracts and Property Management Other Provisions Relating to Public Contracts...

Relação da idade óssea com antropometria e aptidão física em jovens praticantes de voleibol

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Suzet de Araujo Tinoco Cabral

2016-03-01

Full Text Available Resumo O estudo teve como objetivo correlacionar a idade óssea, os componentes antropométricos e a aptidão física em 149 crianças de 8 a 14 anos. Foram aferidos estatura, massa corporal, perímetros de braço e de perna, perímetros de braço e de perna corrigidos, diâmetros bicristailíaco e biacromial, dobras cutâneas tricipital e perna, teste motores de agilidade, velocidade de membros superiores e força explosiva de membros inferiores e superiores e o exame de raios X de mão e punho para verificar maturação. Nos resultados, encontramos correlação positiva entre a idade óssea e as demais variáveis, com exceção das dobras cutâneas tricipital, de perna e teste de agilidade. Dessa forma, verificamos a influência da maturação sobre componentes antropométricos e motores. Assim, possibilitamos uma melhor forma de controle dos processos de detecção, seleção e orientação esportiva.

10 CFR 60.1 - Purpose and scope.

Science.gov (United States)

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Purpose and scope. 60.1 Section 60.1 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES General Provisions § 60.1 Purpose and scope. This part prescribes rules governing the licensing (including issuance...

La portata etica della tragedia tra Bernard Williams e Martha Nussbaum

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Francesco Testini

2015-11-01

Full Text Available Questo breve scritto si propone di ripercorrere sinteticamente alcuni nodi fondamentali del dibattito filosofico tra Bernard Williams e Martha Nussbaum sul significato e sul valore etico della rappresentazione tragica non tanto all’interno del mondo greco quanto per la contemporaneità. Il campo su cui questa analisi intende muoversi è delimitato principalmente da due testi fondamentali come Vergogna e Necessità e l’introduzione alla nuova edizione del 2001, insieme alla prima parte, de La fragilità del bene. Si cercherà di mostrare lo scarto tra queste due diverse concezioni riconducendo ad ognuna il particolare atteggiamento etico che sostiene; di questi diversi punti di vista si cercherà di ricostruire il nesso con le differenti interpretazioni che i due autori forniscono dell’Agamennone di Eschilo e con le diverse reazioni che motivano nei confronti del protagonista e della sua vicenda. Gli obiettivi delle argomentazioni che seguono sono principalmente due: innanzitutto evidenziare come in alcune delle critiche mosse dalla filosofa americana al collega britannico siano rinvenibili spunti fondamentali per quella che si ritiene una più completa comprensione della tragedia come caso limite del conflitto tra soggetto etico e mondo e, in secondo luogo, produrre una giustificazione filosofica ulteriore della reazione motivata da Nussbaum davanti alla condotta di Agamennone che sia compatibile con l’approccio di Williams attraverso una riproposizione del conflitto di valori in termini di conflitto di ruoli.

Exact Tandem Repeats Analyzer (E-TRA): A new program for DNA ...

Indian Academy of Sciences (India)

Unknown

Advanced user defined parameters/options let the researchers use different minimum motif repeats ... E-TRA, we used 5,465,605 human EST sequences derived from 18,814,550 ..... repeat rates of T-cells, embryo and testis were higher.

Small-Caliber Ammunition Identification Guide. Volume 1. Small-Arms Cartridges Up to 15 mm

Science.gov (United States)

1978-06-01

ti acer - T trac~aute, traCLur, Traiong, traceri traccusc T[ R ElD. tir r6duitc. Gallery practicc, surhcalibcr TI’traiqan ce pcrforaiute AP-T V. B...Mukden Arsenal in 1929. ý,A A242 A248 SWEDEN MALAYSIA 2 Made at Maricbcrg Ammunition 1973. Factory. 1925-1928, 4 DOMINICAN RLPUBIAC ITALY 60 1960

Abbreviated sampling and analysis plan for planning decontamination and decommissioning at Test Reactor Area (TRA) facilities

International Nuclear Information System (INIS)

1994-10-01

The objective is to sample and analyze for the presence of gamma emitting isotopes and hazardous constituents within certain areas of the Test Reactor Area (TRA), prior to D and D activities. The TRA is composed of three major reactor facilities and three smaller reactors built in support of programs studying the performance of reactor materials and components under high neutron flux conditions. The Materials Testing Reactor (MTR) and Engineering Test Reactor (ETR) facilities are currently pending D/D. Work consists of pre-D and D sampling of designated TRA (primarily ETR) process areas. This report addresses only a limited subset of the samples which will eventually be required to characterize MTR and ETR and plan their D and D. Sampling which is addressed in this document is intended to support planned D and D work which is funded at the present time. Biased samples, based on process knowledge and plant configuration, are to be performed. The multiple process areas which may be potentially sampled will be initially characterized by obtaining data for upstream source areas which, based on facility configuration, would affect downstream and as yet unsampled, process areas. Sampling and analysis will be conducted to determine the level of gamma emitting isotopes and hazardous constituents present in designated areas within buildings TRA-612, 642, 643, 644, 645, 647, 648, 663; and in the soils surrounding Facility TRA-611. These data will be used to plan the D and D and help determine disposition of material by D and D personnel. Both MTR and ETR facilities will eventually be decommissioned by total dismantlement so that the area can be restored to its original condition

Tra cinema e fumetto: due usi del montaggio

Directory of Open Access Journals (Sweden)

Giuseppe Saitta

2016-01-01

Full Text Available Entrambi linguaggi visivi, ove la narrazione si dipana grazie al rapporto di interdipendenza cheogni singola immagine intrattiene con l'immagine che la precede e quella che le succede. Pur dovendo ricorrere dunque a un montaggio di immagini, fumetto e cinema presentano tra di loro grandi differenze, ma anche grandi similitudini. Cercheremo di scoprire quali sono affidandoci all'analisi della duplice trasposizione di "300" da parte di Frank Miller e Zack Snyder.

Percepção da comunidade nipo-brasileira residente em Curitiba sobre o cadastro de medula óssea The perception of the bone marrow registry of Brazilian Japanese descendants living in the city of Curitiba, Brazil

Directory of Open Access Journals (Sweden)

Alexandra M. Watanabe

2010-01-01

Full Text Available O transplante de medula óssea é uma terapia de escolha utilizada para o tratamento de doenças hematológicas. Entrementes, a probabilidade de se encontrarem doadores compatíveis na população em geral é relativamente baixa. Felizmente, há uma maior probabilidade de se encontrar um doador compatível em doadores de grupos étnicos semelhantes aos do paciente. Especificamente quanto aos nipo-brasileiros, existem poucos virtuais doadores cadastrados no Hemepar de Curitiba. Frente a essa situação, este estudo teve por objetivo conhecer a percepção dos nipo-brasileiros residentes em Curitiba sobre o Cadastro Nacional de Doadores de Medula Óssea e as possíveis causas da pequena adesão a este cadastro. O estudo foi realizado com 400 nipo-brasileiros, entrevistados aleatoriamente por meio de um questionário semiestruturado, no período de abril a junho de 2008. Os resultados foram tabelados pelo Microsoft Office Excel 2003, e as análises estatísticas realizadas pelo Software Statgraphics, versão 5.1. Constatou-se que, mesmo possuindo um bom nível cultural (56% e do bom entendimento sobre a medula óssea (77%, a baixa adesão apresentada para o cadastramento como potencial doador voluntário, principalmente pelas gerações nissei e sansei, deveu-se ao desconhecimento quanto aos locais onde se realizam os cadastros e sobre o processo de doação de medula óssea. Estratégias com vistas a melhorar ainda mais o repasse de informações e esclarecimentos sobre o tema podem ser elaboradas a fim de despertar o interesse e incentivar os potenciais doadores nipo-brasileiros residentes em Curitiba a participar do Cadastro Nacional de Doadores de Medula Óssea. Conhecer as características dos diferentes grupos étnicos que compõem a população brasileira é um indicador que poderá ser utilizado no processo de captação de doadores de medula óssea.Bone marrow transplantation is the therapy of choice for some hematological diseases

Defining the expression of marker genes in equine mesenchymal stromal cells

Directory of Open Access Journals (Sweden)

Deborah J Guest

2008-11-01

Full Text Available Deborah J Guest1, Jennifer C Ousey1, Matthew RW Smith21Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU; 2Reynolds House Referrals, Greenwood Ellis and Partners, 166 High Street, Newmarket, Suffolk, CB8 9WS, UKAbstract: Mesenchymal stromal (MS cells have been derived from multiple sources in the horse including bone marrow, adipose tissue and umbilical cord blood. To date these cells have been investigated for their differentiation potential and are currently being used to treat damage to horse musculoskeletal tissues. However, no work has been done in horse MS cells to examine the expression profile of proteins and cell surface antigens that are expressed in human MS cells. The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from different laboratories and different tissues. At present it is difficult to ascertain whether equivalent cells are being used in different reports. Here, we report on the expression of a range of markers used to define human MS cells. Using immunocytochemistry we show that horse MS cells homogenously express collagens, alkaline phosphatase activity, CD44, CD90 and CD29. In contrast, CD14, CD79α and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen -1, -3, -4, TRA (tumor rejection antigen -1–60 and -1–81 are not expressed. The MS cells also express MHC class I antigens but do not express class II antigens, although they are inducible by treatment with interferon gamma (IFN-γ.Keywords: mesenchymal stem cells, equine, gene expression

Estimativa do risco de traça-verde Palpita vitrealis (Rossi) amplificada por contaminante?

OpenAIRE

Mateus, Helena; Pereira, Cândido; Cardoso, Miguel; Manteigas, Ana; Sequeira, Manuel; Figueiredo, Elisabete; Luz, J.P.; Mexia, António

2012-01-01

A monitorização da população de traça-verde foi efectuada, em olivais da Cova da Beira, em armadilhas com três formulações comerciais de feromona sexual: Russell (em armadilha funil tricolor) e SEDQ e Suterra (ambas em armadilha delta). As contagens, semanais, decorreram: nas armadilhas iscadas com feromona SEDQ, entre Março e Novembro de 2010; nas armadilhas com feromona Russell e Suterra, de Setembro a Novembro. As capturas variaram de 0 a 4 indivíduos/armadilha/semana. Nas armadilhas com S...

Estimativa do risco de Traça-Verde, Palpita vitrealis (Rossi) amplificada por contaminate?

OpenAIRE

Mateus, Helena; Pereira, Cândido; Cardoso, Miguel; Manteigas, Ana; Sequeira, Manuel; Figueiredo, Elisabete; Luz, João Pedro; Mexia, António

2012-01-01

A monitorização da população de traça-verde foi efectuada, em olivais da Cova da Beira, em armadilhas com três formulações comerciais de feromona sexual: Russell (em armadilha funil tricolor) e SEDQ e Suterra (ambas em armadilha delta). As contagens, semanais, decorreram: nas armadilhas iscadas com feromona SEDQ, entre Março e Novembro de 2010; nas armadilhas com feromona Russell e Suterra, de Setembro a Novembro. As capturas variaram de 0 a 4 indivíduos/armadilha/semana. Nas armadilhas com S...

Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in degenerative mice.

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Budd A Tucker

2011-04-01

Full Text Available This study was designed to determine whether adult mouse induced pluripotent stem cells (iPSCs, could be used to produce retinal precursors and subsequently photoreceptor cells for retinal transplantation to restore retinal function in degenerative hosts. iPSCs were generated using adult dsRed mouse dermal fibroblasts via retroviral induction of the transcription factors Oct4, Sox2, KLF4 and c-Myc. As with normal mouse ES cells, adult dsRed iPSCs expressed the pluripotency genes SSEA1, Oct4, Sox2, KLF4, c-Myc and Nanog. Following transplantation into the eye of immune-compromised retinal degenerative mice these cells proceeded to form teratomas containing tissue comprising all three germ layers. At 33 days post-differentiation a large proportion of the cells expressed the retinal progenitor cell marker Pax6 and went on to express the photoreceptor markers, CRX, recoverin, and rhodopsin. When tested using calcium imaging these cells were shown to exhibit characteristics of normal retinal physiology, responding to delivery of neurotransmitters. Following subretinal transplantation into degenerative hosts differentiated iPSCs took up residence in the retinal outer nuclear layer and gave rise to increased electro retinal function as determined by ERG and functional anatomy. As such, adult fibroblast-derived iPSCs provide a viable source for the production of retinal precursors to be used for transplantation and treatment of retinal degenerative disease.

Resultados iniciais do transplante de células de medula óssea para o miocárdio de pacientes com insuficiência cardíaca de etiologia chagásica Early results of bone marrow cell transplantation to the myocardium of patients with heart failure due to chagas disease

Directory of Open Access Journals (Sweden)

Fábio Vilas-Boas

2006-08-01

Full Text Available OBJETIVO: Avaliar efeitos precoces do transplante de células de medula óssea para o miocárdio de pacientes com insuficiência cardíaca (IC de etiologia chagásica. MÉTODOS: A amostra consistiu de 28 pacientes, idade média 52,2±9,9 anos, 24 masculinos, 25 em classe NYHA III e 3 em NYHA IV, apesar de tratamento otimizado. O procedimento consistiu na aspiração de 50ml de medula óssea, separação da fração mononuclear e injeção intracoronariana. Foram avaliados os efeitos sobre a fração de ejeção ventricular esquerda (FEVE, distância no teste de 6 minutos, qualidade de vida, classe NYHA, efeitos arritmogênicos e bioquímicos. RESULTADOS: Não houve complicações relacionadas diretamente ao procedimento. A fração de ejeção ventricular esquerda em repouso antes do transplante era 20,1±6,8% e, após 60 dias, aumentou para 23,0±9,0%, p = 0,02. Houve melhora da classe NYHA (3,1±0,3 para 1,8±0,5; pOBJECTIVE: To evaluate early effects of bone marrow cell transplantation to the myocardium of patients with heart failure (CHF due to Chagas disease. METHODS: We studied 28 patients (mean age 52.2 ± 9.9, of whom 24 were male. Despite optimized treatment, 25 patients were in NYHA class III and three patients, in NYHA class IV. The procedure consisted of aspiration of 50 mL of bone marrow, separation of the mononuclear fraction, and intracoronary injection. Effects on left ventricle ejection fraction (LVEF, distance walked in the six-minute walking test, quality-of-life, NYHA class, arrhythmogenic and biochemical parameters, were all evaluated. RESULTS: There were no complications directly related to the procedure. Baseline left ventricular ejection fraction was 20.1 ± 6.8%, and 60 days after transplantation it increased to 23.0 ± 9.0%, p = 0.02. Significant improvements were observed in the NYHA class (3.1 ± 0.3 to 1.8 ± 0.5; p < 0.0001; quality-of-life (50.9 ± 11.7 to 21.8 ± 13.4; p < 0.0001; and distance walked in six

Estudo comparativo entre traços de argamassa de revestimento utilizadas em Porto Alegre

OpenAIRE

Eduardo Dubaj

2000-01-01

Existe hoje na construção civil uma grande variedade de traços de argamassa para revestimento. Estes traços são, muitas vezes, utilizados de maneira inadequada, não levando-se em consideração as solicitações a que estes revestimentos serão submetidos. Este uso inadequado é muitas vezes o responsável pelo surgimento de manifestações patológicas que conduzem a diminuição da vida útil de um componente ou de uma edificação como um todo, conduzindo a gastos com sua recuperação e até a total insati...

Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem Cells

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Irma Virant-Klun

2011-01-01

Full Text Available The aim of this study was to confirm the presence of stem cells in the ovarian surface epithelium of patients with premature ovarian failure and no mature follicles and oocytes. In these patients, small round cells of unknown origin expressing SOX-2 marker of pluripotency were observed among the epithelial cells just after the ovarian surface epithelium scraping. These cells were an integral part of the ovarian surface epithelium. When the scraped cells were cultured in a medium with added follicular fluid to provide some ovarian niche, primitive oocyte-like cells and typical round-shaped cell clusters positively stained on alkaline phosphatase, and markers of pluripotency, such as SOX-2 and SSEA-4, were developed. These markers were expressed early and also later in the culture. Single oocyte-like cells expressed genes OCT4A, SOX-2, NANOG, NANOS, STELLA, CD9, LIN28, KLF4, GDF3, and MYC, characteristic for pluripotent stem cells. The results of this study confirmed the presence of putative stem cells in the ovarian surface epithelium of these patients and provided some basis to create a stem cell line in the future.

O EFEITO DA SINVASTATINA EM ALTERAÇÕES CARDIOVASCULARES E NA PERDA ÓSSEA INDUZIDA PELA PERIODONTITE

OpenAIRE

WILLIAN MOREIRA MACHADO

2013-01-01

A periodontite é uma doença inflamatória crônica iniciada e perpetuada por bactérias anaeróbicas Gram-negativas que colonizam a área subgengival. Esta doença é caracterizada pela destruição do tecido periodontal de inserção, reabsorção óssea, infiltração de leucócitos e formação de bolsa periodontal. Estudos mostram um aumento significativo do risco de doenças cardiovasculares, principalmente aterosclerose e hipertensão, entre pessoas com periodontite. Acredita-se que a inflamação sistê...

Modification of the Xe 4d giant resonance by the C60 shell in molecular Xe at C60

International Nuclear Information System (INIS)

Amusia, M. Ya.; Baltenkov, A. S.; Chernysheva, L. V.; Felfli, Z.; Msezane, A. Z.

2006-01-01

It is demonstrated that in photoabsorption of the 4d 10 subshell of a Xe atom in molecular Xe at C 60 , the 4d giant resonance that characterizes the isolated Xe atom is distorted significantly. The reflection of photoelectron waves by the C 60 shell leads to profound oscillations in the photoionization cross section such that the Xe giant resonance is transformed into four strong peaks. Similarly, the angular anisotropy parameters, both dipole and nondipole, are also modified. The method of calculation is based on the approximation of the C 60 shell by an infinitely thin bubble potential that leaves the sum rule for the 4d-electrons almost unaffected, but noticeably modifies the dipole polarizability of the 4d-shell

TraLaLa Blip: Community Music for the Electronically Abled

Science.gov (United States)

Reimann, Randolf

2012-01-01

This article presents a personal reflection on the use of electronic music as a vehicle for community music in a disability setting. In order to do this, I draw on my experiences as the founder, facilitator, producer, tutor, sound artist and musician of TraLaLa Blip. I established the group in 2008, after having worked with people with…

OSSERVAZIONI SU UNO SCAMBIO DI POEMETTI TRA PIETRO STANCOVICH E MARTINO FIORANTI

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Sandro Cergna

2013-01-01

Full Text Available Il lavoro presenta un’analisi comparata tra due poemetti d’occasione in dialetto istroveneto dell’Istria sud-occidentale. I componimenti, scritti nel 1826 dal dignanese Martino Fioranti e dal canonico di Barbana Pietro Stancovich, rappresentano un’importante attestazione della presenza di un’attività poetica in quella che rappresentava all’epoca la koiné del territorio istriano, cioè l’idioma di diretta derivazione veneziana. I poemetti rivestono scarsa importanza a livello letterario. Più interessanti si rivelano, invece, se osservati da una prospettiva storica e documentaristica, attraverso la quale ci si svela l’ambiente, il costume dell’epoca, e soprattutto il rapporto di cordiale amicizia che univa i due poeti. In chiusura del lavoro si dà un breve confronto tra il poemetto e i tre sonetti di Martino Fioranti, rinvenuti nell’archivio della Biblioteca universitaria di Pola.

Placas ósseas confeccionadas a partir de diáfise cortical equina na osteossíntese femoral em coelhos

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Fernando Pinheiro Milori

2013-10-01

Full Text Available A evolução tecnológica tem possibilitado o desenvolvimento e o aperfeiçoamento de novos materiais para implantes. Apesar dos fundamentais benefícios providos pelos dispositivos ortopédicos, complicações decorrentes de corrosão, degradação, infecção, além de outras podem ocorrer. O entendimento das características dos biomateriais é fundamental para a previsibilidade do seu comportamento in vivo, fornecendo subsídios para que o composto mais adequado seja escolhido na reconstrução do defeito ósseo. As placas de origem metálica são as mais utilizadas para o reparo de fraturas de ossos longos, sendo mecanicamente resistentes e biocompatíveis. No entanto, a necessidade de remoção e o enfraquecimento do osso são suas principais desvantagens. Neste trabalho, placas produzidas a partir de osso cortical equino foram empregadas experimentalmente em fêmur osteotomizado de coelhos (Grupo osso-GO, num estudo comparativo com placas de metal (Grupo metal-GM. A avaliação radiográfica foi realizada a cada 30 dias, durante 120 dias, momento em que foi então realizada análise histológica do material em estudo. Não houve diferença estatisticamente significante entre os dois grupos com relação à morfometria do calo ósseo e consolidação óssea em todos os momentos avaliados, sendo que ambas as placas permitiram a consolidação em todos os animais. Entretanto, observou-se que o calo ósseo foi menor no GO, em relação ao GM, em todos os momentos do estudo. Por outro lado, a maior parte dos animais do GO apresentou consolidação completa da fratura aos 90 dias, enquanto que no GM isto ocorreu aos 60 dias. Não foram evidenciadas células do tipo corpo estranho na histopatologia dos animais do GO, mas maior quantidade de tecido fibroso foi identificada, envolvendo este biomaterial. A placa confeccionada com osso equino representa uma alternativa de baixo custo e muito viável, uma vez que permitiu estabilização adequada

Work life balance tra limiti istituzionali e pratiche innovative

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Giovanna Campanella

2017-12-01

Full Text Available Come rispondono le politiche di conciliazione cura-lavoro alle esigenze delle lavoratrici e dei lavoratori di nuova generazione: sempre più precari, poveri e instabili dal punto di vista reddituale e/o professionale? Con un sostanziale immobilismo politico.  Questo è il quadro che viene fuori da una rassegna delle principali politiche sociali italiane volte a supporto della genitorialità e alla garanzia dell’occupazione femminile. Il contributo mette in luce i limiti di tali politiche e coglie gli aspetti innovativi di pratiche che si sviluppano dal basso.  Al contempo, una riflessione al margine lascia aperta una importante questione sul rapporto tra flessibilità e conciliazione, ovvero tra organizzazione del lavoro e tempi di cura per la famiglia. Nell’era della rivoluzione digitale,  individuamo forti resistenze al cambiamento, sia da parte delle istituzioni, ma anche e soprattutto dal mondo produttivo, che vanta si modelli “agili” di forme di lavoro da un punto di vista formale (vedi il lavoro agile/ smart working ma che dal punto di vista sostanziale rimane radicato a modelli di contrattazione standar che non agevola né la condizioine lavorativa da un punto di vista di tutele né la conciliaizone cura lavoro e quindi l’occupazione femminile.

Utilização da tração halo-craniana pré-operatória no tratamento de deformidades vertebrais de alto valor angular Uso de tracción halo gravitacional en el tratamiento quirúrgico de las deformidades graves de la columna vertebral Halo gravity preoperative traction in severe spine deformities treatment

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Maximiliano Aguiar Porto

2010-09-01

Full Text Available OBJETIVOS: avaliar o resultado do tratamento cirúrgico das deformidades acentuadas da coluna vertebral por meio da utilização da tração halo-gravitacional. MÉTODOS: foram estudados 17 pacientes portadores de diferentes patologias (6 congênitas, 2 pós-neoplasia, 2 neurofibromatoses, 1 idiopática, 1 raquitismo e 5 neuromusculares submetidos a cirurgia para correção de deformidade de alto valor angular na coluna vertebral. A idade no momento da cirurgia variou de 6 a 26 anos (média de 13,75 anos±4,5. Os parâmetros clínicos avaliados foram o sexo, a idade, o tempo e o peso da tração. Os parâmetros radiográficos estudados foram: a medida da escoliose, da cifose e a altura da coluna vertebral. Os parâmetros radiográficos foram avaliados no período pré-operatório, durante a tração, e no pós-operatório. RESULTADOS: o tempo de tração variou de 14 a 106 dias (34,5±20,83 e a porcentagem de peso da tração em relação ao peso corporal variou de 12,5 a 50% (30%±9,36. A média inicial das escolioses foi de 98,6±25,7º(medida Cobb, reduzido para 83,5º±28,5º durante a tração e para 77º±29,4º no pós-operatório, com a média de correção de 27º, sendo 63% destes ocorridos no período do uso do halo. A média inicial da cifose foi de 86,3±32,08º (Cobb, reduzido para 62,2º±19,9º durante a tração e para 60º±20,9º no pós-operatório, com a média de correção de 26º, sendo 92% durante a tração. CONCLUSÃO: o tratamento cirúrgico das deformidades acentuadas da coluna vertebral, por meio da tração halo-gravitacional pré-operatória, é um método que melhora o ângulo de Cobb reduzindo a correção aguda intraoperatória assim como seus riscos de lesão neurológica.OBJETIVOS: evaluar el resultado del tratamiento quirúrgico en deformidades graves de la columna vertebral con el uso de tracción halo gravitacional. MÉTODOS: fueron estudiados 17 pacientes portadores de diferentes patologías (seis cong

Abdominal adipose tissue distribution in obese children. US versus CT measurements; Valutazione della distribuzione del tessuto adiposo addominale nei bambini obesi. Confronto tra ecografia e Tomografia Computerizzata

Energy Technology Data Exchange (ETDEWEB)

Ferrozzi, F.; Zuccoli, G.; Tognini, G.; Castriota-Scanderbeg, A.; Bacchini, E. [Parma Univ., Parma (Italy). Ist. di Scienze Radiologiche; Bernasconi, S. [Modena Univ., Modena (Italy). Clinica Pediatrica; Campani, R. [Pavia Univ., Pavia (Italy). Ist. di Radiologia

1999-12-01

Computed Tomography (CT) and, more recently, ultrasound (US), have proved excellent tools for quantifying adipose tissue distribution. Body fat distribution is an important factor in the treatment of obesity and its complications. In this work it is investigated the correlation between CT and US measurements in pediatric obesity. Forty obese children and adolescents aged 4.1-14.8 years were submitted to CT and US. Intra-abdominal, subcutaneous and total body fat were calculated (in cm{sup 2}), with the CT image analysis software. The rectus muscle-spine and rectus muscle-aorta distances, as indicative of visceral fat thickness, were measured on US images with(out) compression. The distance between skin fat and fat-rectus muscle interfaces was measured as subcutaneous fat thickness. US-CT findings have been compared with other morphometric variables-i.e., patient's (ideal) body weight and skin fold measures. A statistically significant correlation was found between the CT measurement of visceral fat and the aorta-rectus muscle and rectus muscle-spine distances (r=0.80 and 0.74, respectively). The US measurements of subcutaneous fat were correlated with CT subcutaneous fat area (r=0.82). No correlation was found between overweight, as calculated by body mass index, and CT or US fat. In conclusions, the findings indicate that US is as useful as CT in evaluating body fat distribution in pediatric obesity. [Italian] La TC e piu' recentemente l'ecografia si sono dimostrate eccellenti tecniche di valutazione della quantita' e della distribuzione del tessuto adiposo corporeo. La compartimentazione del grasso corporeo ha, infatti, importanti implicazioni per il trattamento dell'obesita' e delle sue complicanze. Scopo dello studio e' stato quello di correlare i risultati ottenuti con la TC con quelli ecografici nella valutazione dell'obesita' del paziente pediatrico. Quaranta bambini obesi con eta' compresa tra 4,1 e 14

Comprometimento da medula óssea e eosinofilia na paracoccidioidomicose Bone marrow involvement associated with eosinophilia in paracoccidioidomycosis

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M.A. Shikanai-Yasuda

1992-04-01

Full Text Available São descritos 3 casos de paracoccidioidomicose com a forma aguda da doença, nos quais formas leveduriformes de Paracoccidioides brasiliensis foram visualizadas ao exame direto de medula óssea, sendo a cultura também positiva em um caso. Salienta-se o acometimento do sistema fagocítico-mononuclear e a ausência de resposta às provas cutâneas de hipersensibilidade tardia a antígenos microbianos e de P. brasiliensis em todos, bem como a gravidade do quadro clínico e lesões ósseas generalizadas em um caso, com 20.260 eosinófilos/mm³ no sangue periférico. Os autores discutem o possível papel do eosinófilo na interação hospedeiro-parasita na paracoccidioidomicose, sugerindo que a ativação de subpopulação TH 2 e o aumento de secreção de IL 5 e de GM-CSF possam estar relacionados à grande eosinofilia presente no caso mais graveThe authors described three acute paracoccidioidomycosis patients with bone marrow involvement. P. brasiliensis yeast forms were observed in bone marrow smears of all them, and in one case, culture also revealed fungus growth. The mononuclear phagocytic sistem involvement, the blood eosinophilia and the negative skin hipersensibility responses were emphasized in all of them, as well as the severity of the disease in one case, with disseminated bone lesions and 20.260 eosinophils/mm³ in peripheral blood. The authors discuss the possible role of eosinophil in the host-parasite interaction in paracoccidioidomycosis, suggesting that TH 2 subpopulation activation and increased IL 5 and GM-CSF secretions may be responsible by eosinophilia in the most severe case

Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS

KAUST Repository

Wong, Yee-Chin

2016-08-22

Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic therapy rarely results in complete eradication of the pathogen due to its intrinsic resistance to many clinically available antibiotics. Recent attention has turned to the identification of essential genes as the proteins encoded by these genes may serve as potential targets for development of novel antimicrobials. In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing) as a genome-wide screening tool to facilitate the identification of B. cenocepacia genes essential for its growth and viability. A transposon mutant pool consisting of approximately 500,000 mutants was successfully constructed, with more than 400,000 unique transposon insertion sites identified by computational analysis of TraDIS datasets. The saturated library allowed for the identification of 383 genes that were predicted to be essential in B. cenocepacia. We extended the application of TraDIS to identify conditionally essential genes required for in vitro growth and revealed an additional repertoire of 439 genes to be crucial for B. cenocepacia growth under nutrient-depleted conditions. The library of B. cenocepacia mutants can subsequently be subjected to various biologically related conditions to facilitate the discovery of genes involved in niche adaptation as well as pathogenicity and virulence.

Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS

KAUST Repository

Wong, Yee-Chin; Abd El Ghany, Moataz; Naeem, Raeece; Lee, Kok-Wei; Tan, Yung-Chie; Pain, Arnab; Nathan, Sheila

2016-01-01

Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic therapy rarely results in complete eradication of the pathogen due to its intrinsic resistance to many clinically available antibiotics. Recent attention has turned to the identification of essential genes as the proteins encoded by these genes may serve as potential targets for development of novel antimicrobials. In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing) as a genome-wide screening tool to facilitate the identification of B. cenocepacia genes essential for its growth and viability. A transposon mutant pool consisting of approximately 500,000 mutants was successfully constructed, with more than 400,000 unique transposon insertion sites identified by computational analysis of TraDIS datasets. The saturated library allowed for the identification of 383 genes that were predicted to be essential in B. cenocepacia. We extended the application of TraDIS to identify conditionally essential genes required for in vitro growth and revealed an additional repertoire of 439 genes to be crucial for B. cenocepacia growth under nutrient-depleted conditions. The library of B. cenocepacia mutants can subsequently be subjected to various biologically related conditions to facilitate the discovery of genes involved in niche adaptation as well as pathogenicity and virulence.

Vestite alla turchesca. Travestimenti orientali nella drammaturgia italiana e francese tra Sei e Settecento

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Stefano Agostino Moretti

2011-11-01

Full Text Available Da secoli il Mar Mediterraneo rappresenta un confine tra due mondi. Gli orientalisti li chiamavano e continuano a chiamarli mondo “arabo” e mondo “cristiano”. Questo contributo vuole mostrare come, in tempi non sospetti, l’Italia rappresentasse un ponte tra queste due culture. Grazie agli strumenti della storiografia contemporanea, attenta alla vita quotidiana e agli “archivi del corpo”, scopriamo che durante il XVI e il XVII secolo gli incontri tra musulmani e cristiani fossero molto frequenti e spesso felici. Il teatro comico italiano fu il genere letterario che, prima e meglio di ogni altro, riuscì a cogliere e a descrivere tale fenomeno con tutte le implicazioni politiche, religiose e identitarie, restituendone in pieno uno degli elementi sovente sottaciuti e censurati, ovvero la perturbante carica erotica del “diverso”. Uno degli espedienti che riassumono con maggiore efficacia questo  rapporto identitario e culturale è rappresentato dall’uso del travestimento. Paragonando alcune commedie italiane della prima metà del Seicento con il teatro “orientalista” di Bonarelli, Molière e Goldoni, possiamo osservare la nascita e la crescita di una visione stereotipata e libresca dell’altro, là dove i “panni turcheschi” generavano negli spettatori paura e desiderio.

Candidate essential genes in Burkholderia cenocepacia J2315 identified by genome-wide TraDIS

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Yee-Chin Wong

2016-08-01

Full Text Available Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic therapy rarely results in complete eradication of the pathogen due to its intrinsic resistance to many clinically available antibiotics. Recent attention has turned to the identification of essential genes as the proteins encoded by these genes may serve as potential targets for development of novel antimicrobials. In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing as a genome-wide screening tool to facilitate the identification of B. cenocepacia genes essential for its growth and viability. A transposon mutant pool consisting of approximately 500,000 mutants was successfully constructed, with more than 400,000 unique transposon insertion sites identified by computational analysis of TraDIS datasets. The saturated library allowed for the identification of 383 genes that were predicted to be essential in B. cenocepacia. We extended the application of TraDIS to identify conditionally essential genes required for in vitro growth and revealed an additional repertoire of 439 genes to be crucial for B. cenocepacia growth under nutrient-depleted conditions. The library of B. cenocepacia mutants can subsequently be subjected to various biologically related conditions to facilitate the discovery of genes involved in niche adaptation as well as pathogenicity and virulence.

‘Tarasca’. Tra Napoli, Venezia e l’Europa

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Piermario Vescovo

2014-12-01

Full Text Available This paper reconsiders some important episodes of the history of drama and theater of the imagination spread between Naples and Madrid or more generally between Italy and Spain with reference to the Tarasca (procession of Corpus Domini, ideally through six ‘stations’. In this Spanish procession we find on a female dragon the effigies of Gennaro and Gaetano Sacchi, uncle and father of the more famous Antonio, whose stories and travels are one of the matters of this paper. The other matters are focused on the relationship between Carlo Goldoni and the Neapolitans Domenico Barone and Domenico Lalli, and on the Venetian reinvention of Pulcinella’s mask by Giandomenico Tiepolo, passing through Madrid.Col riferimento a una processione legata prevalentemente al Corpus Domini, attraverso sei ‘stazioni’ ideali, il contributo riconsidera alcuni rilevanti episodi di storia dello spettacolo e di diffusione dell’immaginazione teatrale tra Napoli e Madrid, o più in generale tra l’Italia e la Spagna. Nella processione madrilena – sopra un drago femmina semovente (la Tarasca, appunto – si ritrovano le effigi di Gennaro e Gaetano Sacchi, zio e padre del più celebre Antonio, le cui vicende e le cui peregrinazioni costituiscono il filo principale del racconto; gli altri sono costituiti dai rapporti di Carlo Goldoni coi napoletani Domenico Barone e Domenico Lalli e dalla reinvenzione veneziana – passando per Madrid – della maschera di Pulcinella da parte di Giandomenico Tiepolo.

Bases ósseas e musculares dos cortes comerciais da cauda de jacaré-do-Pantanal (Caiman yacare Daudin 1802

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S.I.S. Figueiredo

2015-06-01

Full Text Available A exploração comercial de jacaré-do-Pantanal (Caiman yacare constitui importante cadeia produtiva no Estado de Mato Grosso. As características nutricionais e representatividade na massa corporal de crocodilianos tornaram a região da cauda objeto de estudos morfofisiológicos, evolutivos e tecnológicos. Como inexiste a caracterização anatômica dos músculos e ossos que constituem os cortes comerciais dessa região, objetivou-se descrever os músculos e correspondentes bases ósseas da cauda. Na descrição óssea, foram utilizados um exemplar adulto e seis juvenis. Para caracterização muscular, 24 espécimes juvenis foram conservados em freezer e dissecados a fresco, em ambos os antímeros, para verificação de simetria de ocorrência, fixações musculares, relacões de sintopia, forma e arquitetura muscular. As vértebras caudais são procélicas, exceto a primeira da série, e possuem na superfície ventral do corpo áreas para articulação com os processos hemais, exceto a primeira e as quatro ou cinco últimas. Os cortes comerciais da região são o filé de cauda, composto pelos músculos semiespinhal caudal, longuíssimo caudal, ilioisquiocaudal, caudofemoral longo, transverso e profundo da cauda, enquanto o corte ponta de cauda é constituído pelos músculos longuíssimo caudal e ilioisquiocaudal, com as cinco ou seis últimas vértebras caudais.

Le Kāmasūtra et la « pulsion shastrique » en Inde

OpenAIRE

Naudou, Elizabeth

2013-01-01

Le mot « shastrique » fait référence au traité (śāstra) d’où sont issus les Kāmasūtra, (plus généralement appelé le Kāmasūtra), « aphorismes sur l’amour » : le Kāmaśāstra, « traité sur l’amour ». Qui dit śāstra, dit, en Inde, exposé religieux ou scientifique, d’où l’expression « pulsion shastrique » que l’on pourrait rendre par « pulsion encyclopédique énumérative et classificatoire ». Le but de cet exposé est de replacer le texte dans cette structure mentale typiquement indienne, qui reflète...

Bone marrow transplantation: graft versus host disease and oral changes = Transplante de medula óssea: doença enxerto versus hospedeiro e alterações orais

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Lima, Emeline das Neves de Araújo

2012-01-01

Conclusão: Diante da prevalência de alterações orais relativamente alta associada à DEVH em pacientes submetidos ao TMO, o presente estudo confirma a necessidade de se considerar a odontologia no exame, diagnóstico, tratamento e prognóstico de possíveis complicações após o transplante de medula óssea

Uso de inseticidas para o controle da traça-do-tomateiro e traça-das-crucíferas: um estudo de caso Use of insecticides for controlling the South American Tomato Pinworm and the Diamondback Moth: a case study

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Marina Castelo Branco

2001-03-01

Full Text Available Em agosto de 1999, produtores de tomate e brássicas do Núcleo Rural da Taquara tiveram seus cultivos seriamente comprometidos devido à impossibilidade de controle da traça-do-tomateiro e da traça-das-crucíferas. Diversos inseticidas, alguns com o mesmo princípio ativo ou pertencentes ao mesmo grupo químico, eram aplicados de uma a sete vezes por semana sem qualquer eficiência no controle das pragas. Lavouras foram abandonadas em diferentes estádios de desenvolvimento. A fim de definir uma estratégia de controle que viabilizasse a produção de tomate e brássicas na região, foi avaliado em laboratório a eficiência da dose comercial de alguns inseticidas usados no controle das duas pragas. Para isso, foram coletadas duas populações de traça-do-tomateiro e uma população de traça-das-crucíferas. Para traça-do-tomateiro, cartap, abamectin, lufenuron, acefate e deltametrina causaram respectivamente 100, 90, 67, 2 e 0% de mortalidade das larvas. Para traça-das-crucíferas, B. thuringiensis, abamectin, cartap, acefate and deltametrina causaram 100; 96; 86; 79 e 5% de mortalidade respectivamente. De acordo com estes resultados foi recomendada a suspensão imediata do uso de piretróides e organofosforados para o controle das duas pragas. Abamectin e cartap foram recomendados para o controle da traça-do-tomateiro e B. thuringiensis para o controle de traça-das-crucíferas.In August 1999, at the "Núcleo Rural da Taquara", Federal District, Brazil, tomato and brassica crops were severely damaged by the South American Tomato Pinworm (Tuta absoluta and the Diamondback Moth (Plutella xylostella. During that time growers related that they had been spraying insecticides one to seven times per week without controlling the pests. In the fields it was observed that there were crops with different ages and levels of chemical residues which allowed the pests to multiplicate continuously. Then it was decided that the first step to solve the

Efeitos de extratos de Nierembergia veitchii (Hook Solanaceae sobre a fertilidade de ratas e morfologia óssea dos fetos The effects of Nierembergia veitchii (Hook Solanaceae extracts in rat fertility and fetal skeleton morphology

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João Roberto Braga de Mello

2000-06-01

Full Text Available Os efeitos da administração oral dos extratos aquoso (NvH2O, metanólico (NvmeOH, butanólico (Nvbut e hexano (Nvhex, obtidos, seqüencialmente, de 500g de Nierembergia veitchii (planta seca, foram comparados aos obtidos com vitamina D3 (2,5mg.kg-1 e aos de um grupo controle (solução fisiológica - SF, quando administrados, durante todo o período de gestação (21 dias, a ratas albinas Wistar. A redução do ganho de peso foi evidente nas fêmeas tratadas com Nvhex, Nvbut e vitamina D3. Nesses grupos, houve redução do número de implantes uterinos (9,3 ± 1,4; 9,6 + 1,4 e 8,4 ± 1,4, quando comparados com o controle (SF (11,2 ± 0,4. Essa redução também foi observada no número de filhotes por ninhada (8,1 ± 1,3; 9,0 ± 1,5; 5,1 ± 1,7 e 10,3 ± 0,6, e na massa corporal dos filhotes ao nascer (2,1g ± 0,5; 2,9g ± 0,4; 1,9g ± 0,1 e 3,3g ± 0,1. Os resultados obtidos com NvH2O e NvmeOH não diferiram dos obtidos com o grupo controle. Anomalias macroscópicas foram observadas em fetos de ratas tratadas com Nvhex e Nvbut, 0,1 e 0,2% respectivamente. Os resultados da análise da morfologia óssea dos fetos mostraram a ocorrência de 33,3%; 45%; 9,8% e 11,1% de anomalias para os grupos NvH2O, NvmeOH, Nvbut e SF, respectivamente. As anomalias ósseas mais freqüentemente observadas ocorreram no crânio e constaram de ossificação incompleta de supraoccipital, interparietais, parietais, frontal e temporal.The effects of oral administration of aqueous (NvH2O, methanolic (NvmeOH, buthanolic (Nvbut and hexan (Nvhex extracts, sequentialy obtained from 500g Nierembergia veitchii (dried plant were compared with a vitamin D3 treated group and with a control group (saline, when administered during the pregnancy to Wistar rats (21 days. Weigth gain was reduced in dams treated with Nvhex, Nvbut and vitamin D3. In these groups it was observed a reduction in uterine implantations (9.3 ± 1.4, 9.6 ± 1.4 and 8.4 ± 1.4 when compared with the control

Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices.

Science.gov (United States)

Sharma, Manjinder; Dubey, Pawan K; Kumar, Rajesh; Nath, Amar; Kumar, G Sai; Sharma, G Taru

2013-05-01

Use of somatic cells as a feeder layer to maintain the embryonic stem cells (ESCs) in undifferentiated state limits the stem cell research design, since experimental data may result from a combined ESCs and feeder cell response to various stimuli. Therefore, present study was designed to evaluate the developmental competence of the buffalo ESCs over different homogenous feeders and compare with various extracellular matrices using different concentrations of LIF. Inner cell masses (ICMs) of in vitro hatched blastocysts were cultured onto homologous feeders viz. fetal fibroblast, granulosa and oviductal cell feeder layers and synthetic matrices viz. fibronectin, collagen type I and matrigel in culture medium. Developmental efficiency was found higher for ESCs cultured on fetal fibroblast and granulosa layers (83.33%) followed by fibronectin (77.78%) at 30 ng LIF. Oviductal feeder was found to be the least efficient feeder showing only 11.11% undifferentiated primary ESC colonies at 30 ng LIF. However, neither feeder layer nor synthetic matrix could support the development of primary colonies at 10 ng LIF. Expression of SSEA- 4, TRA-1-60 and Oct-4 were found positive in ESC colonies from all the feeders and synthetic matrices with 20 ng and 30 ng LIF. Fetal fibroblast and granulosa cell while, amongst synthetic matrices, fibronectin were found to be equally efficient to support the growth and maintenance of ESCs pluripotency with 30 ng LIF. This well-defined culture conditions may provide an animal model for culturing human embryonic stem cells in the xeno-free or feeder-free conditions for future clinical applications.

Dopamine-induced SULT1A3/4 promotes EMT and cancer stemness in hepatocellular carcinoma.

Science.gov (United States)

Zou, Juan; Li, Hong; Huang, Qianling; Liu, Xiaomin; Qi, Xiaoxiao; Wang, Ying; Lu, Linlin; Liu, Zhongqiu

2017-10-01

Hepatocellular carcinoma has the second highest incidence rate among malignant cancers in China. Hepatocellular carcinoma development is complex because of the metabolism disequilibrium involving SULT1A3/4, a predominant sulfotransferase that metabolizes sulfonic xenobiotics and endogenous catecholamines. However, the correlation between SULT1A3/4 and hepatocellular carcinoma progression is unclear. By utilizing immunofluorescence and immunohistochemical analysis, we found that in nine hepatocellular carcinoma clinical specimens, SULT1A3/4 was abundantly expressed in tumor tissues compared to that in the adjacent tissues. Moreover, liver cancer cells (HepG2, MHCC97-L, and MHCC97-H) had higher basal expression of SULT1A3/4 than immortalized liver cells (L02 and Chang liver). Ultra-high-pressure liquid chromatography-tandem mass spectrometry assay results further revealed that the concentration of dopamine (a substrate of SULT1A3/4) was negatively correlated with SULT1A3/4 protein expression. As a transcriptional regulator of SULT1A3/4 in turn, dopamine was used to induce SULT1A3/4 in vitro. Interestingly, dopamine significantly induced SULT1A3/4 expression in liver cancer HepG2 cells, while decreased that in L02 cells. More importantly, the expression levels of epithelial-mesenchymal transition biomarkers (N-cadherin and vimentin) and cell stemness biomarkers (nanog, sox2, and oct3/4) considerably increased in HepG2 with dopamine-induced SULT1A3/4, whereas in L02, epithelial-mesenchymal transition and cancer stem cell-associated proteins were contrarily decreased. Furthermore, invasion and migration assays further revealed that dopamine-induced SULT1A3/4 dramatically stimulated the metastatic capacity of HepG2 cells. Our results implied that SULT1A3/4 exhibited bidirectional effect on tumor and normal hepatocytes and may thus provide a novel strategy for hepatocellular carcinoma clinical targeting. In addition, SULT1A3/4 re-expression could serve as a biomarker for

Fast 4D cone-beam CT from 60 s acquisitions

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David C. Hansen

2018-01-01

Full Text Available Background & purpose: Four dimensional Cone beam CT (CBCT has many potential benefits for radiotherapy but suffers from poor image quality, long acquisition times, and/or long reconstruction times. In this work we present a fast iterative reconstruction algorithm for 4D reconstruction of fast acquisition cone beam CT, as well as a new method for temporal regularization and compare to state of the art methods for 4D CBCT. Materials & methods: Regularization parameters for the iterative algorithms were found automatically via computer optimization on 60 s acquisitions using the XCAT phantom. Nineteen lung cancer patients were scanned with 60 s arcs using the onboard image on a Varian trilogy linear accelerator. Images were reconstructed using an accelerated ordered subset algorithm. A frequency based temporal regularization algorithm was developed and compared to the McKinnon-Bates algorithm, 4D total variation and prior images compressed sensing (PICCS. Results: All reconstructions were completed in 60 s or less. The proposed method provided a structural similarity of 0.915, compared with 0.786 for the classic McKinnon-bates method. For the patient study, it provided fewer image artefacts than PICCS, and better spatial resolution than 4D TV. Conclusion: Four dimensional iterative CBCT reconstruction was done in less than 60 s, demonstrating the clinical feasibility. The frequency based method outperformed 4D total variation and PICCS on the simulated data, and for patients allowed for tumor location based on 60 s acquisitions, even for slowly breathing patients. It should thus be suitable for routine clinical use.

41 CFR 60-3.4 - Information on impact.

Science.gov (United States)

2010-07-01

... 3-UNIFORM GUIDELINES ON EMPLOYEE SELECTION PROCEDURES (1978) General Principles § 60-3.4 Information... the continuation of patterns of assignments of incumbent employees caused by prior discriminatory... minority or female candidates to be atypical of the normal pool of applicants from that group. Where the...

Comportamentos de risco para os transtornos alimentares e traços perfeccionistas em atletas de atletismo

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Leonardo de Sousa Fortes

Full Text Available Resumo O objetivo do estudo foi analisar a influência do perfeccionismo nos comportamentos de risco para os transtornos alimentares de atletas adolescentes do sexo feminino da modalidade atletismo. Fizeram parte do estudo 52 atletas da modalidade de atletismo de clubes da cidade de São Paulo/SP com idade entre 12 e 17 anos. Utilizaram-se as subescalas do Eating Attitudes Test (EAT-26 e a Multidimensional Perfectionism Scale (MPS para avaliar os comportamentos de risco para os transtornos alimentares e os traços perfeccionistas, respectivamente. Conduziu-se a regressão linear múltipla stepwise para analisar a influência dos perfeccionismo nos comportamentos de risco para os transtornos alimentares. Os resultados indicaram influência da MPS nos escores das subescalas Dieta (F(1, 44 = 5,74; p = 0,05 e Autocontrole Oral (F(1, 44 = 6,13; p = 0,04 do EAT-26. No entanto, não foi evidenciado impacto da MPS nos escores da subescala Bulimia e Preocupação com 0 Alimenta (F(1, 44 = 1,26; p = 0,22. Assim, em razão da investigação apresentar delineamento transversal, pressupõe-se que as atletas de atletismo com traços perfeccionistas podem estar mais susceptíveis para a restrição alimentar e a influência ambiental para a ingesta alimentar.

Indigenous Engagement in Tropical River Research in Australia: The TRaCK Program

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Sue E. Jackson

2015-05-01

Full Text Available The literature on scientific-Indigenous ecological knowledge collaborations rarely analyses programmatic efforts undertaken by multi-disciplinary research groups over very large geographic scales. The TRaCK (Tropical Rivers and Coastal Knowledge research program was established to provide the science and knowledge needed by governments, industries, and communities to sustainably manage northern Australia’s rivers and estuaries. A number of policies and procedures were developed to ensure that the needs of Indigenous people of the multi-jurisdictional region were addressed and to enhance the benefits they might derive from participating in the research. An overarching Indigenous Engagement Strategy undergirded the program’s engagement activities, providing guidance on matters relating to the protection of intellectual property, negotiation of research agreements, remuneration for Indigenous expertise, and communications standards. This article reviews the achievements and shortcomings of the TRaCK experience of Indigenous engagement and highlights lessons for researchers and research organisations contemplating applied environmental science initiatives of this scale and scope.

AA.VV., La Giustizia nella Letteratura e nello Spettacolo Siciliani tra ...

African Journals Online (AJOL)

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ruolo e i significati attribuiti al concetto di Giustizia nella. Letteratura e nello Spettacolo Siciliani tra '800 e '900. Da Verga a Sciascia costituisce il volume che raccoglie, a cura di Angelo. Zappulla, gli Atti del convegno su questo fondamentale tema. La centralità del tema Giustizia, nelle più diverse forme del fervore culturale ...

Contribuição do plasma rico em plaquetas na reparação óssea de defeitos críticos criados em crânios de camundongos Platelet-rich plasma contribute to the process of bone repair of critical defects created in the calvaria of mice

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Betânia Souza Monteiro

2010-07-01

Full Text Available No presente estudo, foram avaliados, de forma macro e microscópica, os resultados da aplicação do PRP em defeitos ósseos críticos de 6,0mm de diâmetro confeccionados em calvária de 24 camundongos isogênicos C57BL/6 jovens, separados em dois grupos experimentais. O grupo controle não recebeu tratamento, e no grupo tratado foram depositados, no interior do defeito, 50,0µL plasma em gel contendo 1,0x10(9 plaquetas. Constatou-se que o gel de PRP autólogo depositado em defeitos críticos contribuiu positivamente para o processo de reparação óssea, mormente na fase inicial.This study aimed to evaluate macro and microscopic results after PRP application in bone critical defects of 6.0mm in diameter realized in cavarial of twenty-four young isogenic mice C57BL/6. Control group didn't receive treatment and in the treated group it was deposited 50.0µL of plasma gel containing 1.0x10(9 platelets in the defects. It was found that autologous PRP gel contributed positively to the bone repair process, especially in initial phase.

41 CFR 60-1.8 - Segregated facilities.

Science.gov (United States)

2010-07-01

... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Segregated facilities. 60...; Compliance Reports § 60-1.8 Segregated facilities. To comply with its obligations under the Order, a contractor must ensure that facilities provided for employees are provided in such a manner that segregation...

Flexibility of interval between vaccinations with AS03A-adjuvanted influenza A (H1N1 2009 vaccine in adults aged 18–60 and >60 years: a randomized trial

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Duval Xavier

2012-07-01

Full Text Available Abstract Background Flexibility of vaccination schedule and lower antigen content can facilitate pandemic vaccine coverage. We assessed the immune response and safety of AS03-adjuvanted A/California/7/2009 H1N1 pandemic vaccine containing half of the registered adult haemagglutinin (HA antigen content, administered as a two-dose schedule at intervals of 21 days or 6 months in both young and elderly adults. Methods In this open-label randomized trial, healthy adults aged 18–60 years (N = 163 and >60 years (N = 143 received AS03A-adjuvanted A/California/7/2009 H1N1 vaccine containing 1.9 μg HA on Day 0. A second dose was given on Day 21 (n = 177 or Day 182 (n = 106. Haemagglutination-inhibition (HI antibody responses were analyzed on Days 0, 21, 42, 182, 364 and additionally on Day 203 for subjects vaccinated on Day 182. Solicited and unsolicited adverse events were recorded. Results The HI antibody response in both age strata 21 days after the first dose met and exceeded all regulatory acceptance criteria although the results suggested a lower response in the older age stratum (geometric mean titres [GMTs] for HI antibodies of 420.5 for subjects aged 18–60 years and 174.4 for those >60 years. A second dose of AS03A adjuvanted A/H1N1/2009 vaccine induced a further increase in antibody titres and the response was similar whether the second dose was administered at 21 days (GMTs of 771.8 for 18–60 years and 400.9 for >60 years or 6 months (GMTs of 708.3 for 18–60 years and 512.1 for >60 years following the first dose. Seroprotection rates remained high at 6 months after one dose or two doses while at 12 months rates tended to be higher for the 6 month interval schedule (93.3% for 18–60 years and 80.4% for >60 years than the 21 day schedule (82.3% for 18–60 years and 50.0% for >60 years. Reactogenicity/safety profiles were similar for both schedules, there was no evidence of an

Tra ufficialità e Samizdat: Viktor Sosnora

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Noemi Albanese

2015-07-01

Full Text Available La figura di Viktor Sosnora si presenta come una delle più particolari nel panorama letterario della Leningrado della seconda metà del Novecento. A metà tra mondo ufficiale e quello dell'underground, fu anche uno dei pochissimi autori ai quali, in epoca sovietica, fu concesso di viaggiare in Europa ed entrare in contatto con il mondo intellettuale coevo. La sua ricerca estetica e stilistica parte dalle tematiche antico russe per innovarle dall'interno grazie ad un gusto per la lingua estremamente contemporaneo;ciò si tradusse in sperimentazioni poetiche sempre più ardite che resero impossibile la pubblicazione ufficiale dei suoi testi, divenuti invece oggetto di culto negli ambienti del Samizdat.

Densidade mineral óssea de adolescentes com sobrepeso e obesidade Bone mineral density in overweight and obese adolescents

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Fernanda Cobayashi

2005-08-01

Full Text Available OBJETIVO: Estudar a densidade óssea como fator concomitante da obesidade em adolescentes pós-púberes, controlando outras variáveis que possam interferir nessa relação. MÉTODOS: Estudo com 83 sobrepesos e obesos (IMC > P85 e 89 não obesos (P5 OBJECTIVE: to study bone density as a concomitant factor for obesity in post-pubertal adolescents, controlling for other variables that may interfere in such a relation. METHODS: Study comprising 83 overweight and obese adolescents (BMI > P85 and 89 non obese ones (P5 < BMI < P85. Cases and controls were selected out of 1,420 students (aged 14-19 from a public school in the city of São Paulo. The bone mineral density of the lumbar spine (L2-L4 in g/cm² was assessed by dual-energy x-ray absorptiometry (LUNAR™ DPX-L. The variable bone density was dichotomized using 1.194 g/cm² as cutoff point. Bivariate analyses were conducted considering the prevalence of overweight and obesity followed by multivariate analysis (logistic regression according to a hierarchical conceptual model. RESULTS: The prevalence of bone density above the median was twice more frequent among cases (69.3% than among controls (32.1%. In the bivariate analysis such prevalence resulted in an odds ratio (OR of 4.78. The logistic regression model showed that the association between obesity and mineral density is yet more intense with an OR of 6.65 after the control of variables related to sedentary lifestyle and intake of milk and dairy products. CONCLUSION: Obese and overweight adolescents in the final stages of sexual maturity presented higher bone mineral density in relation to their normal-weight counterparts; however, cohort studies will be necessary to evaluate the influence of such characteristic on bone resistance in adulthood and, consequently, on the incidence of osteopenia and osteoporosis at older ages.

Os cuidados de enfermagem ao paciente pós-transplantado de medula óssea

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Marina Assunção Rocha

2012-04-01

Full Text Available O transplante de medula óssea (TMO é um procedimento médico complexo utilizado atualmente no tratamento de uma série de enfermidades graves. É indicado no tratamento de patologias malignas ou não, modificando o prognóstico do paciente em questão. Porém, não é um método plenamente resolutivo, podendo curar o paciente ou levá-lo a morte. Este estudo tem como objetivos a elucidação dos principais conceitos relacionados ao tema e a identificação de um planejamento assistencial do enfermeiro que contemple a etapa de pós TMO. Os resultados obtidos relacionam a fase do pós-transplante às necessidades específicas dos pacientes e o plano assistencial de enfermagem, evidenciando a necessidade de um suporte profissional que englobe os âmbitos social e psicológico do paciente.

Técnicas e sítios de coleta de medula óssea em cães e gatos Techniques and bone marrow sites sampling in dogs and cats

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Daniel Curvello de Mendonça Müller

2009-10-01

Full Text Available Atualmente, o incremento das pesquisas enfoca a medula óssea como fonte potencial para o tratamento de doenças, uma vez que seu uso como diagnóstico já é largamente empregado. Os objetivos deste artigo são revisar os melhores acessos à medula óssea e destacar a importância desse material como diagnóstico e tratamento de doenças. São abordadas particularidades sobre as técnicas de coleta e as complicações observadas pelos autores, a fim de facilitar os procedimentos, tornando-os mais seguros.Currently, the research focuses on bone marrow as a potential source for the treatment of diseases, since its use as a diagnosis is already largely employed. This paper aims to promote a practical review on the best access to the bone marrow and the importance of this material as diagnosis and treatment. Sampling techniques are observed and detailed by the authors, in order to facilitate the procedures and make them safer.

Reconhecer e traduzir traços de Sêneca em Shakespeare

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Renata Cazarini de Freitas

2014-07-01

  O filósofo Sêneca (c.4 a.C.-65 d.C. é autor de oito tragédias latinas às quais se atribui, regra geral, elevado grau de “influência” na literatura dramática produzida na Inglaterra da virada do século XVI para o XVII. Sem recorrer ao usual método de localizar citações ou equivalências textuais, propõe-se aqui reconhecer os traços da elocução senequiana em Shakespeare a partir da identificação de dispositivos da retórica antiga e de suas correspondências em cenas específicas de Richard III, Hamlet e The Tempest. A tradução dessas peças a partir do latim ou do inglês deve refletir o domínio e a intenção dos autores no uso de tais expedientes retóricos.

The equation of state of n-pentane in the atomistic model TraPPE-EH

Science.gov (United States)

Valeev, B. U.; Pisarev, V. V.

2018-01-01

In this work, we study the vapor-liquid equilibrium in n-pentane. We use the TraPPE-EH (transferable potentials for phase equilibria-explicit hydrogen) forcefield, where each hydrogen and carbon atom is considered as independent center of force. The fluid behavior was investigated with different values of density and temperature by molecular dynamics method. The n-pentane evaporation curve was calculated in the temperature range of 290 to 390 K. The densities of the coexisting phases are also calculated. The compression curve at 370 K was calculated and isothermal bulk modulus was found. The simulated properties of n-pentane are in good agreement with data from a database of the National Institute of Standards and Technology, so the TraPPE-EH model can be recommended for simulations of hydrocarbons.

Awakened by cellular stress: isolation and characterization of a novel population of pluripotent stem cells derived from human adipose tissue.

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Saleh Heneidi

Full Text Available Advances in stem cell therapy face major clinical limitations, particularly challenged by low rates of post-transplant cell survival. Hostile host factors of the engraftment microenvironment such as hypoxia, nutrition deprivation, pro-inflammatory cytokines, and reactive oxygen species can each contribute to unwanted differentiation or apoptosis. In this report, we describe the isolation and characterization of a new population of adipose tissue (AT derived pluripotent stem cells, termed Multilineage Differentiating Stress-Enduring (Muse Cells, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. Muse-AT cells grow in suspension as cell spheres reminiscent of embryonic stem cell clusters. Muse-AT cells are positive for the pluripotency markers SSEA3, TR-1-60, Oct3/4, Nanog and Sox2, and can spontaneously differentiate into mesenchymal, endodermal and ectodermal cell lineages with an efficiency of 23%, 20% and 22%, respectively. When using specific differentiation media, differentiation efficiency is greatly enhanced in Muse-AT cells (82% for mesenchymal, 75% for endodermal and 78% for ectodermal. When compared to adipose stem cells (ASCs, microarray data indicate a substantial up-regulation of Sox2, Oct3/4, and Rex1. Muse-ATs also exhibit gene expression patterns associated with the down-regulation of genes involved in cell death and survival, embryonic development, DNA replication and repair, cell cycle and potential factors related to oncogenecity. Gene expression analysis indicates that Muse-ATs and ASCs are mesenchymal in origin; however, Muse-ATs also express numerous lymphocytic and hematopoietic genes, such as CCR1 and CXCL2, encoding chemokine receptors and ligands involved in stem cell

CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

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Mihalescu-Maingot Maria

2010-02-01

Full Text Available Abstract Background Tumor initiating cells (TICs provide a new paradigm for developing original therapeutic strategies. Methods We screened for TICs in 47 human adult brain malignant tumors. Cells forming floating spheres in culture, and endowed with all of the features expected from tumor cells with stem-like properties were obtained from glioblastomas, medulloblastoma but not oligodendrogliomas. Results A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs. Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide. Conclusions Our results further highlight the specificity of a subset of high-grade gliomas, MGNT. TICs derived from these tumors represent a new tool to screen for innovative therapies.

CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

International Nuclear Information System (INIS)

Patru, Cristina; Berhneim, Alain; Mihalescu-Maingot, Maria; Haiech, Jacques; Bièche, Ivan; Moura-Neto, Vivaldo; Daumas-Duport, Catherine; Junier, Marie-Pierre; Chneiweiss, Hervé; Romao, Luciana; Varlet, Pascale; Coulombel, Laure; Raponi, Eric; Cadusseau, Josette; Renault-Mihara, François; Thirant, Cécile; Leonard, Nadine

2010-01-01

Tumor initiating cells (TICs) provide a new paradigm for developing original therapeutic strategies. We screened for TICs in 47 human adult brain malignant tumors. Cells forming floating spheres in culture, and endowed with all of the features expected from tumor cells with stem-like properties were obtained from glioblastomas, medulloblastoma but not oligodendrogliomas. A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs). Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide. Our results further highlight the specificity of a subset of high-grade gliomas, MGNT. TICs derived from these tumors represent a new tool to screen for innovative therapies

Influência da auto-percepção do envelhecimento e dos traços de personalidade na satisfação com a reforma

OpenAIRE

Fragoso, Nuno Miguel do Rosário Mendes

2013-01-01

Tese de mestrado, Psicologia (Secção de Psicologia Clínica e da Saúde - Núcleo de Psicologia Clínica Dinâmica), Universidade de Lisboa, Faculdade de Psicologia, 2013 O presente trabalho foca-se no estudo da influência da auto-perceção do envelhecimento e dos traços de personalidade na satisfação com a reforma, numa amostra de idosos oriundos da comunidade e sem psicopatologia diagnosticada. Tem como objetivos: (1) analisar a auto-perceção do envelhecimento, os traços de personalidade e a s...

Estudo de resistência à tração de três sistemas adesivos associados a resina composta em superfícies dentinárias

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MATOS Adriana Bona

2001-01-01

Full Text Available Este trabalho teve como objetivo comparar a resistência à tração de três sistemas adesivos associados a resina composta aderidos à superfície dentinária. Quarenta e cinco superfícies de dentina foram obtidas a partir de molares humanos extraídos, sobre elas foi preparado "smear layer" e foram divididas em 3 grupos: G1 adesivo autocondicionante + resina micro-híbrida; G2 adesivo de componente único + ácido e resina micro-híbrida e G3 adesivo convencional (ácido + "primer" + bond + resina micro-híbrida, aplicados de acordo com as instruções dos fabricantes. Os corpos-de-prova foram preparados para teste de tração, imersos em água destilada por 24 horas a 37ºC. Findo este tempo, o teste de tração foi realizado em máquina de ensaio Inströn com velocidade constante de 0,5 mm/min. Os resultados foram expressos em MPa e submetidos a análise de variância (p < 0,05. Os autores concluíram que: o tipo de sistema adesivo influenciou na resistência à tração das resinas compostas testadas; o sistema adesivo de componente único apresentou os melhores resultados de resistência adesiva.

Retalho ósseo pré-fabricado com osso homógeno: estudo da maturação óssea em um modelo experimental Prefabricated homogenous bone flap: bone maturation study as an experimental model

Directory of Open Access Journals (Sweden)

Alfredo Benjamim Duarte da Silva

2000-01-01

Full Text Available As opções convencionais de tratamento de perdas ósseas, como enxertos e retalhos não são satisfatórias. Com isso uma nova categoria de retalhos, o retalho pré-fabricado (RPF, passou a ser estudada. Foram retirados 42 ossos metatársicos-I das patas traseiras de 21 ratos Wistar. O grupo I (n=21 foi descalcificado e liofilizado e o grupo II (n=21 foi não descalcificado e congelado a -70°C. Após 3 semanas os ossos foram enxertados em 21 animais sobre os vasos epigástricos inferiores nas regiões inguinal direita e esquerda, individualizados por uma lâmina de silicone. Os grupos I e II foram divididos em três subgrupos cada, de acordo com o tempo de permanência (1, 2 e 4 semanas após a pré-fabricação do retalho. No estudo histológico macroscópico os grupos apresentaram diferenças em morfologia e consistência: o grupo I mostrou perda da arquitetura óssea e da rigidez e grupo II mantiveram sua forma, rigidez e consistência. Na análise qualitativa alterações foram observadas nos fragmentos do grupo desmineralizado e liofilizado, especialmente no subgrupo de 2 semanas. O grupo II não apresentou alteração no aspecto do tecido ósseo mineralizado entre os diversos subgrupos. É nítida a diferença histológica entre os RPF com osso descalcificado e liofilizado quando comparado ao não descalcificado e congelado. A reabsorção óssea gradual sugere que a rotação do retalho deve ocorrer precocemente. Este estudo demonstra a aplicabilidade do osso homógeno para pré-fabricação de retalhos.As conventional options for bone repair are not satisfactory, a new flap category was developed, the prefabricated flap (RPF. 42 metatarsic-I bone were obtained from 21 Wistar rats, and divided in two groups: group I (n=21 the bones were freeze- dryed and decalcified and group II (n=21 was frozen to - 70º C. After 3 weeks the bones were grafted in 21 rats, onto the left na right inferior epigastric vessels wrapped in silicon sheet

Esters of Quinoxaline 1ˏ4-Di-N-oxide with Cytotoxic Activity on Tumor Cell Lines Based on NCI-60 Panel

Science.gov (United States)

Rivera, Gildardo; Ahmad Shah, Syed Shoaib; Arrieta-Baez, Daniel; Palos, Isidro; Mongue, Antonio; Sánchez-Torres, Luvia Enid

2017-01-01

Quinoxalines display diverse and interesting pharmacological activities as antibacterial, antiviral, antiparasitic and anticancer agents. Particularly, their 1ˏ4-di-N-oxide derivatives have proved to be cytotoxic agents that are active under hypoxic conditions as that of solid tumours. A new series of quinoxaline 1ˏ4-di-N-oxide substitutes at 7-position with esters group were synthetized and characterized by infrared (IR), proton nuclear magnetic resonance (1H-NMR), spectroscopy, and elemental analysis. Seventeen derivatives (M1-M3, E1-E8, P1-P3 and DR1-DR3) were selected and evaluated for antitumor activities using the NCI-60 human tumor cell lines screen. Results showed that E7, P3 and E6 were the most active compounds against the cell lines tested. Substitutions at 7-position with esters group not necessarily affect the biological activity, but the nature of the esters group could exert an influence on the selectivity. Additionally, substitutions at 2-position influenced the cytotoxic activity of the compounds. PMID:29201086

Resistência à tração entre amálgama e resina composta em diferentes condições de oxidação - estudo in vitro

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YOUSSEF Michel Nicolau

1999-01-01

Full Text Available Este trabalho estuda in vitro a resistência à tração de corpos-de-prova de amálgama-resina composta unidos pelo adesivo dental Scotchbond Multi-Uso Plus. Os corpos-de-prova foram divididos em três grupos de dez, sendo que cada corpo era o resultado da união de dois discos, um de amálgama e outro de resina composta. Esses discos foram obtidos dentro de cilindros de baquelite e sofreram acabamento superficial com lixa de grana 600. Pretendeu-se simular a técnica do amalcomp e verificar a resistência à tração entre o amálgama e a resina composta em diferentes condições de oxidação do amálgama. Dos resultados obtidos e mediante análise de variância e teste de Tukey a 5%, pôde-se concluir que: o Grupo 3, que ficou na saliva artificial por trinta dias e portanto sofreu maior oxidação, apresenta resistência à tração superior e estatisticamente significante em relação aos grupos 1 e 2; o Grupo 2, que ficou na saliva por sete dias e sofreu menor oxidação, não apresenta resistência à tração estatisticamente superior ao Grupo 1 de amálgama recém-condensado.

Condutas desviantes e traços de personalidade: testagem de um modelo causal

OpenAIRE

Vasconcelos,Tatiana Cristina; Gouveia,Valdiney Veloso; Pimentel,Carlos Eduardo; Pessoa,Viviany Silva

2008-01-01

A meta principal deste estudo foi comprovar a adequação de um modelo causal à explicação de comportamentos socialmente desviantes (condutas anti-sociais e delitivas), considerando a contribuição dos traços de personalidade (neuroticismo, extroversão e busca de sensações). Para tanto, participaram 755 estudantes do Ensino Médio e Superior, sendo a maioria do sexo feminino (50,3%), de escolas privadas (53,0%) e com idades variando de 16 a 26 anos (média=20,1; desvio-padrão=3,12). Estes responde...

Progettazione e disciplina dell’esoscheletro : l'inestricabile co-evoluzione tra umani e robot

NARCIS (Netherlands)

Fosch Villaronga, Eduard; Özcan, Beste; Pirni, A.

2017-01-01

Quest’articolo descrive come sia stretto il legame tra esseri umani e l’evoluzione degli esoscheletri in termini di design, di regolamentazione e di necessità umane. Mentre gli attuali esoscheletri sono ingombranti e pesanti, i progressi nei materiali e nel design concettuale cambieranno

Densidade Mineral Óssea de Mulheres na Pós-menopausa com e sem Antecedente de Histerectomia com Conservação Ovariana Bilateral Bone Mineral Density in Postmenopausal Women with and without Previous Hysterectomy with Bilateral Ovarian Conservation

Directory of Open Access Journals (Sweden)

João Daniel Hobeika

2000-09-01

Full Text Available Objetivo: avaliar a densidade mineral óssea de mulheres na pós-menopausa com o antecedente cirúrgico de histerectomia com conservação ovariana bilateral, realizada no menacme, comparadas a um grupo de mulheres com menopausa natural, não-histerectomizadas. Métodos: tratou-se de um estudo de corte transversal, com 30 mulheres histerectomizadas no menacme, avaliadas na pós-menopausa, comparadas a 102 mulheres menopausadas naturalmente, sendo realizado a densitometria óssea (Lunar DPX. Resultados: as médias etárias, índice de massa corporal, cor da pele, tabagismo, escolaridade, menarca, paridade e antecedente de laqueadura tubária foram semelhantes nos grupos estudados. A comparação das médias da densidade mineral óssea e do T-score dos três locais do fêmur, utilizando o teste de Bonferroni, não apresentou diferenças estatisticamente significativas. As médias da densidade mineral óssea e do T-score da coluna lombar foram analisadas pelo teste t de Student e também não mostraram diferenças estatísticas. Conclusão: estes resultados sugerem que a histerectomia com conservação ovariana bilateral, realizada em mulheres no menacme, não parece ocasionar redução adicional da massa óssea, quando avaliadas na pós-menopausa.Purpose: to evaluate the bone mineral density of postmenopausal women with previous hysterectomy and with bilateral ovarian conservation compared to a group of nonhysterectomized naturally menopausal women. Methods: this is a cross-sectional study of bone densitometry (Lunar DPX in 30 menopausal women hysterectomized when in the premenopause compared with 102 naturally postmenopausal women. Results: the mean age, body mass index, color of the skin, smoking habits, educational level, menarche, parity and previous tubal ligation were similar in the studied groups. Bone mineral density average and the T-score of the three femural sites analyzed by the Bonferroni test did not show significant differences. The

La tradizione nella scienza il progresso scientifico come equilibrio dialettico tra idee innovatrici e tradizione in una raccolta di saggi inediti

CERN Document Server

Heisenberg, Werner

1982-01-01

In questa raccolta di saggi (inediti per l'Italia), da lui stesso selezionati poco prima della morte, Heisenberg analizza da varie angolazioni i rapporti tra progresso scientifico e tradizione : la storia della scienza è soprattutto una storia di idee, e da un giusto equilibrio dialettico tra i nuovi concetti e la tradizione, che costituisce la base ideale per discuterli, scaturisce il progress scientifico.

CARM1 modulators affect epigenome of stem cells and change morphology of nucleoli.

Science.gov (United States)

Franek, M; Legartová, S; Suchánková, J; Milite, C; Castellano, S; Sbardella, G; Kozubek, S; Bártová, E

2015-01-01

CARM1 interacts with numerous transcription factors to mediate cellular processes, especially gene expression. This is important for the maintenance of ESC pluripotency or intervention to tumorigenesis. Here, we studied epigenomic effects of two potential CARM1 modulators: an activator (EML159) and an inhibitor (ellagic acid dihydrate, EA). We examined nuclear morphology in human and mouse embryonic stem cells (hESCs, mESCs), as well as in iPS cells. The CARM1 modulators did not function similarly in all cell types. EA decreased the levels of the pluripotency markers, OCT4 and NANOG, particularly in iPSCs, whereas the levels of these proteins increased after EML159 treatment. EML159 treatment of mouse ESCs led to decreased levels of OCT4 and NANOG, which was accompanied by an increased level of Endo-A. The same trend was observed for NANOG and Endo-A in hESCs affected by EML159. Interestingly, EA mainly changed epigenetic features of nucleoli because a high level of arginine asymmetric di-methylation in the nucleoli of hESCs was reduced after EA treatment. ChIP-PCR of ribosomal genes confirmed significantly reduced levels of H3R17me2a, in both the promoter region of ribosomal genes and rDNA encoding 28S rRNA, after EA addition. Moreover, EA treatment changed the nuclear pattern of AgNORs (silver-stained nucleolus organizer regions) in all cell types studied. In EA-treated ESCs, AgNOR pattern was similar to the pattern of AgNORs after inhibition of RNA pol I by actinomycin D. Together, inhibitory effect of EA on arginine methylation and effect on related morphological parameters was especially observed in compartment of nucleoli.

Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells.

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John J Vincent

Full Text Available The cell intrinsic programming that regulates mammalian primordial germ cell (PGC development in the pre-gonadal stage is challenging to investigate. To overcome this we created a transgene-free method for generating PGCs in vitro (iPGCs from mouse embryonic stem cells (ESCs. Using labeling for SSEA1 and cKit, two cell surface molecules used previously to isolate presumptive iPGCs, we show that not all SSEA1+/cKit+ double positive cells exhibit a PGC identity. Instead, we determined that selecting for cKit(bright cells within the SSEA1+ fraction significantly enriches for the putative iPGC population. Single cell analysis comparing SSEA1+/cKit(bright iPGCs to ESCs and embryonic PGCs demonstrates that 97% of single iPGCs co-express PGC signature genes Blimp1, Stella, Dnd1, Prdm14 and Dazl at similar levels to e9.5-10.5 PGCs, whereas 90% of single mouse ESC do not co-express PGC signature genes. For the 10% of ESCs that co-express PGC signature genes, the levels are significantly lower than iPGCs. Microarray analysis shows that iPGCs are transcriptionally distinct from ESCs and repress gene ontology groups associated with mesoderm and heart development. At the level of chromatin, iPGCs contain 5-methyl cytosine bases in their DNA at imprinted and non-imprinted loci, and are enriched in histone H3 lysine 27 trimethylation, yet do not have detectable levels of Mvh protein, consistent with a Blimp1-positive pre-gonadal PGC identity. In order to determine whether iPGC formation is dependent upon Blimp1, we generated Blimp1 null ESCs and found that loss of Blimp1 significantly depletes SSEA1/cKit(bright iPGCs. Taken together, the generation of Blimp1-positive iPGCs from ESCs constitutes a robust model for examining cell-intrinsic regulation of PGCs during the Blimp1-positive stage of development.

Comparison of Hemostasis Times With a Kaolin-Based Hemostatic Pad (QuikClot Radial) vs Mechanical Compression (TR Band) Following Transradial Access: A Pilot Prospective Study.

Science.gov (United States)

Roberts, Jonathan S; Niu, Jianli; Pastor-Cervantes, Juan A

2017-10-01

Hemostasis following transradial access (TRA) is usually achieved by mechanical compression. We investigated use of the QuikClot Radial hemostasis pad (Z-Medica) compared with the TR Band (Terumo Medical) to shorten hemostasis after TRA. Thirty patients undergoing TRA coronary angiography and/or percutaneous coronary intervention were randomized into three cohorts post TRA: 10 patients received mechanical compression with the TR Band, 10 patients received 30 min of compression with the QuikClot Radial pad, and 10 patients received 60 min of compression with the QuikClot Radial pad. Times to hemostasis and access-site complications were recorded. Radial artery patency was evaluated 1 hour after hemostasis by the reverse Barbeau's test. There were no differences in patient characteristics, mean dose of heparin (7117 ± 1054 IU), or mean activated clotting time value (210 ± 50 sec) at the end of procedure among the three groups. Successful hemostasis was achieved in 100% of patients with both the 30-min and 60-min compression groups using the QuikClot pad. Hemostasis failure occurred in 50% of patients when the TR Band was initially weaned at the protocol-driven time (40 min after sheath removal). Mean compression time for hemostasis with the TR Band was 149.4 min compared with 30.7 min and 60.9 min for the 30-min and 60-min QuikClot groups, respectively. No radial artery occlusion occurred in any subject at the end of the study. Use of the QuikClot Radial pad following TRA in this pilot trial significantly shortened hemostasis times when compared with the TR Band, with no increased complications noted.

Traços de resistência na obra de Augusto Roa Bastos

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Carlos Henrique Lopes Almeida

2017-01-01

Full Text Available Este estudo tem como proposta a análise de alguns traços de resistência presentes em algumas das produções literárias do autor paraguaio Augusto Roa Bastos. As obras escolhidas são Vigilia del Almirante (2008 e a trilogia Yo, el supremo (2003, El fiscal (2008 e El hijo del hombre (2003. O primeiro romance trata da volta ao período do descobrimento e desenvolve uma confrontação entre Colombo, os cronistas e o narrador. A trilogia se ocupa do cenários belicosos da história paraguaia e de alguns ditadores e suas relações com o poder. O tratamento dado a essa matéria histórica nas narrativas supracitadas pode ser entendida como traços de resistência, bem como alguns artifícios narrativos aplicados pelo autor. A análise será realizada a partir das discussões sobre Resistência e descobrimento propostos por Galeano (1989, Bitterli (2012 e Grützmacher (2009.

Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms

DEFF Research Database (Denmark)

Kristensen, D.M.; Nielsen, J.E.; Skakkebaek, N.E.

2008-01-01

NANOG and OCT-3/4, UTF-1 and REX-1 are expressed throughout human testes development. The expression pattern indicated that UTF-1 plays a possible role in spermatogonial self-renewal, whereas expression of REX-1 in meiotic cells from both testes and ovary indicate a role in meiosis. UFT-1 and REX-1...... and REX-1 during human gonadal development and in TGCT. METHODS: Expression of UTF-1 and REX-1 was studied in 52 specimens from human gonadal development and in 86 samples from TGCT. RESULTS: UTF-1 and REX-1 were expressed throughout male gonadal development. In the mature testis, UTF-1 was expressed...

Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells.

Science.gov (United States)

Zhang, Jianying; Wang, James H-C

2015-01-01

Aging is known to cause tendon degeneration whereas moderate exercise imparts beneficial effects on tendons. Since stem cells play a vital role in maintaining tissue integrity, in this study we aimed to define the effects of aging and moderate exercise on tendon stem/progenitor cells (TSCs) using in vitro and in vivo models. TSCs derived from aging mice (9 and 24 months) proliferated significantly slower than TSCs obtained from young mice (2.5 and 5 months). In addition, expression of the stem cell markers Oct-4, nucleostemin (NS), Sca-1 and SSEA-1 in TSCs decreased in an age-dependent manner. Interestingly, moderate mechanical stretching (4%) of aging TSCs in vitro significantly increased the expression of the stem cell marker, NS, but 8% stretching decreased NS expression. Similarly, 4% mechanical stretching increased the expression of Nanog, another stem cell marker, and the tenocyte-related genes, collagen I and tenomodulin. However, 8% stretching increased expression of the non-tenocyte-related genes, LPL, Sox-9 and Runx-2, while 4% stretching had minimal effects on the expression of these genes. In the in vivo study, moderate treadmill running (MTR) of aging mice (9 months) resulted in the increased proliferation rate of aging TSCs in culture, decreased lipid deposition, proteoglycan accumulation and calcification, and increased the expression of NS in the patellar tendons. These findings indicate that while aging impairs the proliferative ability of TSCs and reduces their stemness, moderate exercise can mitigate the deleterious effects of aging on TSCs and therefore may be responsible for decreased aging-induced tendon degeneration.

Teriparatida (PTH[1-34]rh: uma nova perspectiva no tratamento da osteoporose

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Oliveira Juliana Helena Abreu de

2003-01-01

Full Text Available No momento, as medicações aprovadas para tratamento da osteoporose agem reduzindo a taxa de perda óssea e diminuindo a reabsorção óssea. A teriparatida é um fragmento recombinante sintético de 34 aminoácidos do hormônio paratireóide humano. A teriparatida se liga ao receptor de PTH da proteína G e estimula a formação e a ação dos osteoblastos, que são as células responsáveis pela formação dos ossos. Assim, a principal diferença entre o tratamento da osteoporose com teriparatida e o tratamento anti-reabsorção é que a teriparatida promove o crescimento de osso novo. Em estudos pré-clínicos, o uso intermitente de PTH foi associado com um aumento significativo da massa óssea gradeada em diversos locais. A exposição intermitente ao PTH durante 4 a 6 semanas em modelos de animais ovariectomizados leva a um aumento da espessura do osso gradeado. Há estudos clínicos que mostram que a teriparatida aumenta significativamente a densidade óssea e diminui a incidência de fraturas osteoporóticas vertebrais e não-vertebrais nas mulheres com osteoporose pós-menopáusica e têm risco alto de fratura, e aumenta a densidade óssea nos homens com osteoporose, tanto hipogonádica como idiopática. A teriparatida é dada por injeção subcutânea diária e foi associada com um mínimo de efeitos colaterais, além de não apresentar interações medicamentosas. Sendo assim, a teriparatida surge como uma abordagem completamente nova no tratamento da osteoporose, estimulando diretamente a formação do osso.

Incerta glòria di Joan Sales tra filologia, storia e traduzione

Directory of Open Access Journals (Sweden)

Patrizio Rigobon

2015-12-01

Full Text Available In questo saggio si fa una riflessione sull’opera di Joan Sales Incerta glòria e particolarmente si vede le conessione tra filologia, storia e traduzione. This article makes a reflection on the work of Joan Sales, Incerta glòria, and it particularly affects the connection between philology, history and translation.

Characterization, isolation and culture of primordial germ cells in domestic animals: recent progress and insights from the ovine species.

Science.gov (United States)

Ledda, S; Bogliolo, L; Bebbere, D; Ariu, F; Pirino, S

2010-09-01

Primordial germ cell (PGC) allocation, characterization, lineage restriction, and differentiation have been extensively studied in the mouse. Murine PGC can be easily identified using markers as alkaline phosphatase content or the expression of pluripotent markers such as Pou5f1, Nanog, Sox2, Kit, SSEA1, and SSEA4. These tools allowed us to clarify certain aspects of the complex interactions of somatic and germinal cells in the establishment of the germ cell lineage, its segregation from the neighbouring somatic tissue, and the guidance mechanisms during migration that direct most of the germ cells into the genital ridges. Few data are available from other domestic animals and here we reported our preliminary studies on the isolation, characterization, and in vitro culture of sheep PGCs. Sheep PGCs can be identified with the markers previously used in mouse, but, in some cases, these markers are not coherently expressed in the same cell depending on the grade of differentiation and on technical problems related to commercial antibodies used. Pluripotency of PGCs in culture (EGCs) from domestic animals also needs further evaluation even though the derivation of embryonic pluripotent cell lines from large mammals may be an advantage as they are more physiologically similar to the human and perhaps more relevant for clinical translation studies. Comprehensive epigenetic reprogramming of the genome in early germ cells, and derived EGCs including extensive erasure of epigenetic modifications, may be relevant for gaining insight into events that lead to reprogramming and establishment of totipotency. EGCs can differentiate in vitro in a various range of tissues, form embryonic bodies, but in many cases failed to generate tumours when transplanted into immunodeficient mice and are not able to generate germline chimeric animals after their transfer. Such incomplete information clearly indicates the urge to improve the studies on derivation of stem cells in farm animals and

(E,E,E)-1.3.5-Tris[4-acetylsulfanyl)-styryl]benzene toluene hemisolvate

DEFF Research Database (Denmark)

Sørensen, H.O.; Magnussen, M.; Stuhr-Hansen, N.

2005-01-01

The first crystal structure of a three-terminal sulfur end-capped oligophenylenevinylene, C36H30O3S3 x 0.5C7H8, has been determined at 122 (1) K. The molecular threefold symmetry is not utilized in the crystal structure. It is confirmed that the double bonds have been fully transformed into a tra...

41 CFR 105-60.305-1 - Definitions.

Science.gov (United States)

2010-07-01

... to the public and to private sector organizations; (2) Ensure that groups and individuals pay the... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Definitions. 105-60.305..., Orders, Policies, Interpretations, Manuals, and Instructions § 105-60.305-1 Definitions. For the purpose...

RNA-Binding Protein L1TD1 Interacts with LIN28 via RNA and is Required for Human Embryonic Stem Cell Self-Renewal and Cancer Cell Proliferation

OpenAIRE

Närvä, Elisa; Rahkonen, Nelly; Emani, Maheswara Reddy; Lund, Riikka; Pursiheimo, Huha-Pekka; Nästi, Juuso; Autio, Reija; Rasool, Omid; Denessiouk, Konstantin; Lähdesmäki, Harri; Rao, Anjana; Lahesmaa, Ritta

2012-01-01

Human embryonic stem cells (hESC) have a unique capacity to self-renew and differentiate into all the cell types found in human body. Although the transcriptional regulators of pluripotency are well studied, the role of cytoplasmic regulators is still poorly characterized. Here, we report a new stem cell-specific RNA-binding protein L1TD1 (ECAT11, FLJ10884) required for hESC self-renewal and cancer cell proliferation. Depletion of L1TD1 results in immediate downregulation of OCT4 and NANOG. F...

Dramatic distortion of the 4d giant resonance by the C{sub 60} fullerene shell

Energy Technology Data Exchange (ETDEWEB)

Amusia, M Ya [Racah Institute of Physics, The Hebrew University, Jerusalem 91904 (Israel); Baltenkov, A S [Arifov Institute of Electronics, Akademgorodok, 700125 Tashkent (Uzbekistan); Chernysheva, L V [A F Ioffe Physical-Technical Institute, St Petersburg 194021 (Russian Federation); Felfli, Z [Department of Physics and Center for Theoretical Studies of Physical Systems, Clark Atlanta University, Atlanta, GA 30314 (United States); Msezane, A Z [Department of Physics and Center for Theoretical Studies of Physical Systems, Clark Atlanta University, Atlanta, GA 30314 (United States)

2005-05-28

The photoionization cross section for the endohedral Xe at C{sub 60} atom is investigated within the framework of representing the C{sub 60} by a delta-type potential. Results demonstrate that in Xe at C{sub 60}, the 4d giant resonance is distorted significantly when compared with that of the isolated Xe atom. The reflection of the photoelectron waves by the C{sub 60} causes strong oscillations in the photoionization cross section resulting in the replacement of the Xe 4d giant resonance by four prominent peaks. The approximation of C{sub 60} by an infinitely thin real potential preserves reasonably well the sum rule for the 4d electrons but modifies the dipole polarizability of the 4d shell. (letter to the editor)

An Examination of Factors Influencing Students Selection of Business Majors Using TRA Framework

Science.gov (United States)

Kumar, Anil; Kumar, Poonam

2013-01-01

Making decisions regarding the selection of a business major is both very important and challenging for students. An understanding of this decision-making process can be valuable for students, parents, and university programs. The current study applies the Theory of Reasoned Action (TRA) consumer decision-making model to examine factors that…

InTraCert. Inception report. The role of an integrated tradable green certificate system in a liberalising market

International Nuclear Information System (INIS)

Boots, M.G.; Schaeffer, G.J.; De Zoeten, C.; Mitchell, C.; Anderson, T.; Morthorst, P.E.; Nielsen, L.; Gual, M.; Del Rio, P.; Cadenas, A.; Hernandez, F.; Kuehn, I.; Braeur, W.; Stronzik, M.

2000-12-01

The InTraCert project aims to explore the possibility of integrating the existing and planned Tradable Green Certificate (TGC) schemes in the European Union and, therefore, creating a plausible unified market for TGCs. Particular attention will be paid to the possibilities of integrating TGCs for green electricity, heat and gas. Furthermore, it intents to examine the possible interactions arising from such a system with more direct GHG abatement measures, i.e. Carbon Emissions Trading (CET). The scope of the InTraCert project requires specific information for EU-15 countries regarding, on the one hand, Renewable Energy Sources (RES) used for electricity, gas and heat generation and. on the other, GHG emission levels and national strategies. In order to account for this information need. specific country inventories have been designed and carried out by InTraCert members in this first phase of the project. The inventory shows that Belgium, Italy, Denmark, the Netherlands, Austria, Sweden and the UK seriously want to implement a TGC system. The systems will indeed be nationally oriented; hardly any provisions for international trade in the different TGCs will be put in place. An essential prerequisite for efficient cross border trade is agreement on the carbon credit that comes with RE production. What is the size of the carbon credit and will this credit be attached to the TGC while traded? These will be the main questions to be answered in the next phase of the InTraCert project. 8 refs

Association between CTLA-4 60G/A and -1661A/G polymorphisms and the risk of cancers: a meta-analysis.

Directory of Open Access Journals (Sweden)

Qing Yan

Full Text Available CTLA-4 is one of the most fundamental immunosuppressive cotykines which belongs to the immunoglobulin super-family, and is expressed mainly on activated T cells. Previous studies have reported the existence of CTLA4 60G/A and CTLA4 -1661A/G polymorphism in cancers. However, the effects remain conflicting. Hence, we performed a meta-analysis to investigate the association between these polymorphisms and cancer risk.We searched the Pubmed and Web of Science databases until October 24, 2013 to obtain relevant published studies. Pooled odds ratios (ORs and corresponding 95% confidence intervals (CIs for the relationship between CTLA4 gene polymorphisms and cancer susceptibility were calculated by stata 11 software. Heterogeneity tests, sensitivity analyses and publication bias assessments were also performed in our meta-analysis.A total of 22 articles comprising 31 case-control studies concerning the CTLA-4 60G/A and CTLA-4 -1661A/G polymorphisms were included in the meta-analysis. The pooled results suggested the CTLA-4 60G/A polymorphism was significantly associated with an increased skin cancer risk (AA vs. GG: OR = 1.32, 95%CI = 1.09-1.59; AA vs. GA+GG: OR = 1.26, 95%CI = 1.07-1.48. For CTLA-4 -1661 A/G polymorphism, the results showed that the CTLA-4 -1661A/G polymorphism was significantly associated with an increased cancer risk (GA vs. AA: OR = 1.44, 95%CI = 1.13-1.82; GA+GG vs. AA: OR = 1.35, 95%CI = 1.07-1.69; G vs. A: OR = 1.21, 95%CI = 1.01-1.47, especially in gastric cancer, breast cancer, other cancers and in Asians population subgroups.Our meta-analysis suggests that the CTLA-4 -1661A/G polymorphism is a potential factor for the susceptibility of cancer, especially in gastric cancer, breast cancer and other cancers, and the CTLA-4 60G/A polymorphism is significantly associated with increased skin cancer risk. The effect of the CTLA-4 -1661A/G polymorphism on cancer susceptibility especially

Numerical modelling of the V-J combinations of the T cell receptor TRA/TRD locus.

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Florence Thuderoz

2010-02-01

Full Text Available T-Cell antigen Receptor (TR repertoire is generated through rearrangements of V and J genes encoding alpha and beta chains. The quantification and frequency for every V-J combination during ontogeny and development of the immune system remain to be precisely established. We have addressed this issue by building a model able to account for Valpha-Jalpha gene rearrangements during thymus development of mice. So we developed a numerical model on the whole TRA/TRD locus, based on experimental data, to estimate how Valpha and Jalpha genes become accessible to rearrangements. The progressive opening of the locus to V-J gene recombinations is modeled through windows of accessibility of different sizes and with different speeds of progression. Furthermore, the possibility of successive secondary V-J rearrangements was included in the modelling. The model points out some unbalanced V-J associations resulting from a preferential access to gene rearrangements and from a non-uniform partition of the accessibility of the J genes, depending on their location in the locus. The model shows that 3 to 4 successive rearrangements are sufficient to explain the use of all the V and J genes of the locus. Finally, the model provides information on both the kinetics of rearrangements and frequencies of each V-J associations. The model accounts for the essential features of the observed rearrangements on the TRA/TRD locus and may provide a reference for the repertoire of the V-J combinatorial diversity.

Numerical Modelling Of The V-J Combinations Of The T Cell Receptor TRA/TRD Locus

Science.gov (United States)

Dariz, Aurélie; Baum, Thierry Pascal; Hierle, Vivien; Demongeot, Jacques; Marche, Patrice Noël; Jouvin-Marche, Evelyne

2010-01-01

T-Cell antigen Receptor (TR) repertoire is generated through rearrangements of V and J genes encoding α and β chains. The quantification and frequency for every V-J combination during ontogeny and development of the immune system remain to be precisely established. We have addressed this issue by building a model able to account for Vα-Jα gene rearrangements during thymus development of mice. So we developed a numerical model on the whole TRA/TRD locus, based on experimental data, to estimate how Vα and Jα genes become accessible to rearrangements. The progressive opening of the locus to V-J gene recombinations is modeled through windows of accessibility of different sizes and with different speeds of progression. Furthermore, the possibility of successive secondary V-J rearrangements was included in the modelling. The model points out some unbalanced V-J associations resulting from a preferential access to gene rearrangements and from a non-uniform partition of the accessibility of the J genes, depending on their location in the locus. The model shows that 3 to 4 successive rearrangements are sufficient to explain the use of all the V and J genes of the locus. Finally, the model provides information on both the kinetics of rearrangements and frequencies of each V-J associations. The model accounts for the essential features of the observed rearrangements on the TRA/TRD locus and may provide a reference for the repertoire of the V-J combinatorial diversity. PMID:20174554

40 CFR 60.482-1 - Standards: General.

Science.gov (United States)

2010-07-01

... of initial startup. (b) Compliance with §§ 60.482-1 to 60.482-10 will be determined by review of... only during startup and shutdown, excluding startup and shutdown between batches of the same campaign... Three quarters Semiannually. 75 to 100 Monthly Quarterly Semiannually. (2) Pumps and valves that are...

Snail1 induces epithelial-to-mesenchymal transition and tumor initiating stem cell characteristics

International Nuclear Information System (INIS)

Dang, Hien; Ding, Wei; Emerson, Dow; Rountree, C Bart

2011-01-01

Tumor initiating stem-like cells (TISCs) are a subset of neoplastic cells that possess distinct survival mechanisms and self-renewal characteristics crucial for tumor maintenance and propagation. The induction of epithelial-mesenchymal-transition (EMT) by TGFβ has been recently linked to the acquisition of TISC characteristics in breast cancer. In HCC, a TISC and EMT phenotype correlates with a worse prognosis. In this work, our aim is to elucidate the underlying mechanism by which cells acquire tumor initiating characteristics after EMT. Gene and protein expression assays and Nanog-promoter luciferase reporter were utilized in epithelial and mesenchymal phenotype liver cancer cell lines. EMT was analyzed with migration/invasion assays. TISC characteristics were analyzed with tumor-sphere self-renewal and chemotherapy resistance assays. In vivo tumor assay was performed to investigate the role of Snail1 in tumor initiation. TGFβ induced EMT in epithelial cells through the up-regulation of Snail1 in Smad-dependent signaling. Mesenchymal liver cancer post-EMT demonstrates TISC characteristics such as tumor-sphere formation but are not resistant to cytotoxic therapy. The inhibition of Snail1 in mesenchymal cells results in decreased Nanog promoter luciferase activity and loss of self-renewal characteristics in vitro. These changes confirm the direct role of Snail1 in some TISC traits. In vivo, the down-regulation of Snail1 reduced tumor growth but was not sufficient to eliminate tumor initiation. In summary, TGFβ induces EMT and TISC characteristics through Snail1 and Nanog up-regulation. In mesenchymal cells post-EMT, Snail1 directly regulates Nanog expression, and loss of Snail1 regulates tumor growth without affecting tumor initiation

First tier modeling of consumer dermal exposure to substances in consumer articles under REACH: a quantitative evaluation of the ECETOC TRA for consumers tool.

Science.gov (United States)

Delmaar, J E; Bokkers, B G H; ter Burg, W; van Engelen, J G M

2013-02-01

The demonstration of safe use of chemicals in consumer products, as required under REACH, is proposed to follow a tiered process. In the first tier, simple conservative methods and assumptions should be made to quickly verify whether risks for a particular use are expected. The ECETOC TRA Consumer Exposure Tool was developed to assist in first tier risk assessments for substances in consumer products. The ECETOC TRA is not a prioritization tool, but is meant as a first screening. Therefore, the exposure assessment needs to cover all products/articles in a specific category. For the assessment of the dermal exposure for substances in articles, ECETOC TRA uses the concept of a 'contact layer', a hypothetical layer that limits the exposure to a substance contained in the product. For each product/article category, ECETOC TRA proposes default values for the thickness of this contact layer. As relevant experimental exposure data is currently lacking, default values are based on expert judgment alone. In this paper it is verified whether this concept meets the requirement of being a conservative exposure evaluation method. This is done by confronting the ECETOC TRA expert judgment based predictions with a mechanistic emission model, based on the well established theory of diffusion of substances in materials. Diffusion models have been applied and tested in many applications of emission modeling. Experimentally determined input data for a number of material and substance combinations are available. The estimated emissions provide information on the range of emissions that could occur in reality. First tier tools such as ECETOC TRA tool are required to cover all products/articles in a category and to provide estimates that are at least as high as is expected on the basis of current scientific knowledge. Since this was not the case, it is concluded that the ECETOC TRA does not provide a proper conservative estimation method for the dermal exposure to articles. An

O gálio e a patologia óssea Gallium and bone pathology

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Petr Melnikov

2008-01-01

Full Text Available PROPOSTA: Revisão de trabalhos científicos referentes à incorporação do gálio no tecido ósseo, ao mecanismo da atividade terapêutica desse elemento, bem como a formação, crescimento e solubilidade da hidroxiapatita na presença dos sais de gálio. JUSTIFICATIVA: Diferente de outras drogas que impedem a perda de cálcio, os sais de elemento traço gálio são eficazes em hipercalcemia severa. O gálio (geralmente na forma de nitrato aumenta a concentração de cálcio e fósforo no osso, influindo nos osteoclastos de maneira direta não tóxica, em doses surpreendentemente baixas. Apesar de que os detalhes do mecanismo de ação do gálio não são bem esclarecidos, está comprovado que esse mecanismo envolve a inserção do gálio na matriz de hidroxiapatita, protegendo-a contra a reabsorção e melhorando as propriedades biomecânicas do sistema esquelético. Este fármaco age também nos componentes celulares do osso, impedindo sua absorção ao diminuir a secreção ácida dos osteoclastos. São necessárias mais publicações sobre o uso do gálio no tratamento de várias doenças onde prevalece esta patologia. CONCLUSÕES: Devido as suas características interessantes e promissoras, o gálio merece ser futuramente avaliado do ponto de vista experimental e clínico, como um agente antiabsortivo em ortopedia, traumatologia e doenças relacionadas com o câncer. Maior conhecimento dos mecanismos envolvidos pode fornecer as idéias para estratégia terapêutica, com o objetivo de diminuir hipercalcemia e perda óssea. Espera-se que novos compostos do gálio sejam desenvolvidos e avaliados clinicamente.PURPOSE: To review the literature concerning the incorporation of gallium into bone tissue, mechanisms of therapeutic activity of this element, as well as the formation, growth and solubility of hydroxiapatite in the presence of gallium salts. JUSTIFICATION: In contrast to other calcium-saving drugs, salts of trace element gallium are

Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.

Science.gov (United States)

Greber, Basil Johannes; Gerhardy, Stefan; Leitner, Alexander; Leibundgut, Marc; Salem, Michèle; Boehringer, Daniel; Leulliot, Nicolas; Aebersold, Ruedi; Panse, Vikram Govind; Ban, Nenad

2016-01-14

Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation. Copyright © 2016 Elsevier Inc. All rights reserved.

Software of microcomputer ELEKTRONIKA-60 installed in the control system of the measuring projector PUOS-4

International Nuclear Information System (INIS)

Dikij, V.M.; Nekipelova, G.D.; Shakhbazyan, V.V.; Yurpalov, V.D.

1990-01-01

The tasks, structure and aspects of realization of servodrivers of the PUOS-4 measuring projector (measuring carriage, tape transport) on the installed ELEKTRONIKA-60 microcomputer operating in the multi-machine system of film information preprocessing, are considered. Realizability of drivers is estimated under rigid operation conditions using the RTM60 real time monitor. The problem of the projector testing is also considered. 11 refs.; 5 figs.; 1 tab

Field Sampling Plan for the HWMA/RCRA Closure Certification of the TRA-731 Caustic and Acid Storage Tank System - 1997 Notice of Violation Consent Order

International Nuclear Information System (INIS)

Evans, S.K.

2002-01-01

This Field Sampling Plan for the HWMA/RCRA Closure Certification of the TRA-731 Caustic and Acid Storage Tank System is one of two documents that comprise the Sampling and Analysis Plan for the HWMA/RCRA closure certification of the TRA-731 caustic and acid storage tank system at the Idaho National Engineering and Environmental Laboratory. This plan, which provides information about sampling design, required analyses, and sample collection and handling procedures, is to be used in conjunction with the Quality Assurance Project Plan for the HWMA/RCRA Closure Certification of the TRA-731 Caustic and Acid Storage Tank System

A correlação de métodos dexa e cdexa em absortimetria mineral óssea The correlation between DEXA and CDEXA methods in bone mineral absorptiometry

Directory of Open Access Journals (Sweden)

Cláudio Gilberto Defavori

2007-06-01

Full Text Available OBJETIVO: Estudar a intercambiabilidade dos resultados de densidade mineral óssea entre máquinas de absortimetria de raios-x em duas energias (DEXA Hologic® e de absortimetria computadorizada de raios-x convencionais em duas energias (CDEXA Cromox®. MATERIAIS E MÉTODOS: Para 38 casos de quadril e 31 casos de coluna lombar avaliados em 43 pacientes atendidos em um centro de diagnóstico por imagem, medidas de densidade mineral óssea foram realizadas em ambas as máquinas. A máquina Cromox® foi calibrada usando-se o phantom Cromox® de referência para absortimetria óssea. RESULTADOS: Forte correlação entre os resultados obtidos nas duas máquinas foi encontrada para cada sítio do esqueleto. O coeficiente de correlação linear medido para o colo femoral direito foi r = 0,920, p 0,95. Para ambos os sítios, a diferença média aritmética em Tscore entre as máquinas é pequena, menor que a menor variação significativa.OBJECTIVE: To study the interchangeability of the bone mineral density results between Hologic® dual energy x-ray absorptiometry (DEXA and Cromox® computed dual energy conventional x-ray absorptiometry (CDEXA equipments. MATERIALS AND METHODS: Thirty-eight femoral necks and 31 lumbar spines of 43 patients underwent bone mineral density measured by both equipments in a diagnostic imaging center. The Cromox® equipment was calibrated with a reference Cromox® phantom for bone absorptiometry. RESULTS: A strong correlation between results obtained from the two devices was found for each skeletal site. The linear correlation coefficient measured for the right femoral neck was r = 0.920, p 0.95. For both sites, the arithmetic mean difference in Tscore between devices is low - least than the lowest significant variance.

Fatores que influenciam a massa óssea de crianças e adolescentes saudáveis mensurada pelo ultrassom quantitativo de falanges: revisão sistemática

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Tathyane Krahenbühl

2014-09-01

Full Text Available Objetivo: Analisar quais os principais fatores que influenciam na massa óssea de crianças e adolescentes avaliada pelo ultrassom quantitativo (QUS de falanges. Fonte de dados: Foi realizada revisão sistemática da literatura, de acordo com o método Prisma, com buscas nas bases de dados do Pubmed/Medline, Bireme e Scielo, referente ao período de 2001 a 2012, nos idiomas inglês e português, utilizando os descritores children, adolescent, ultrassonography finger phalanges, quantitative ultrasound of phalanges, phalangeal quantitative ultrasound. Síntese dos dados: Foram incluídos 21 artigos. As meninas apresentaram no QUS valores de Amplitude Dependent Speed of Sound (AD-SoS superiores aos meninos durante o desenvolvimento puberal. Os valores dos parâmetros do QUS de falanges aumentaram com o incremento do estádio maturacional, assim como ocorre com o Dual-energy X-ray Absorptiometry (DXA. Variáveis antropométricas, como idade, peso, estatura, índice de massa corporal (IMC e massa magra, demonstraram correlações positivas com os valores do QUS de falanges. A atividade física também demonstrou estar positivamente relacionada ao aumento da massa óssea. Fatores como etnia, genética, ingestão calórica e perfil socioeconômico ainda não mostraram relação conclusiva e necessitam um número maior de estudos. Conclusões: O QUS de falanges é um método indicado para avaliar a progressiva aquisição da massa óssea durante o crescimento e a maturação dos indivíduos em fase escolar, por acompanhar as alterações que ocorrem com o aumento da idade e do estádio puberal. Observouse influência positiva, principalmente das variáveis de sexo, maturação, estatura, peso e IMC, sendo seus dados semelhantes quando comparados ao método padrão-ouro, o DXA.

O transplante de medula óssea na leucemia mielóide aguda: análise de 80 pacientes transplantados no complexo do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

OpenAIRE

Bueno,Nadjanara D.; Saboya,Rosaura; Martins,Maria Cristina; Silva,Roberto L.; Chamone,Dalton A. F.; Rocha,Isamara F.; Sturaro,Daniel; Dulley,Frederico L.

2004-01-01

Oitenta pacientes consecutivos portadores de Leucemia Mielóide Aguda (LMA) submetidos a transplante de medula óssea alogênico (TMO alo) e autogênico (TMO auto), foram selecionados entre 1989 e 2001. Quarenta por cento dos mesmos estavam vivos ao final do estudo; no TMO alo 37,9% e no TMO auto 45,4%. Fatores como sexo, classificação Franco-Americano-Britânica de LMA, tratamento de indução, número de células infundidas e regime de condicionamentos não tiveram significância estatística na sobrev...

Densidade mineral óssea sistêmica vs situação clínica periodontal: estudo transversal em mulheres na pós-menopausa Systemic bone mineral density versus clinical periodontal condition: cross-sectional study in postmenopausal women

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Fernanda Ferreira Lopes

2008-10-01

Full Text Available OBJETIVO: Analisar a densidade mineral óssea sistêmica (DMO e a situação periodontal em mulheres na pós-menopausa, visando compreender o papel da osteoporose como um fator de risco à doença periodontal. MÉTODOS: A amostra da pesquisa foi constituída por 47 mulheres na pós-menopausa, que foram divididas em três grupos: 14 com osso normal (G1, 17 no grupo com osteopenia (G2 e 16 pacientes com osteoporose (G3, através da avaliação da densidade mineral óssea (DMO, aferida pela absormetria de dupla emissão com raios-X na área lombar (L1-L4. A condição periodontal foi avaliada pelo índice gengival (IG, índice da placa (IP e o nível de inserção clínica (NIC. Os resultados tabulados foram analisados e submetidos ao tratamento estatístico, através do teste ANOVA a um critério (α=0,05 e o teste de correlação de Pearson (α=0,01. RESULTADOS: Verificou-se não haver diferenças significativas na situação periodontal em mulheres na pós-menopausa, através das variáveis IG, IP e NIC. Observou-se correlação significativa entre os parâmetros periodontais IG, IP e NIC entre si (p0,01. CONCLUSÃO: A situação periodontal em mulheres na pós-menopausa não depende da massa óssea sistêmica, não havendo correlação significativa entre DMO e os parâmetros periodontais, sendo necessárias pesquisas longitudinais para considerar a osteoporose como um fator de risco à doença periodontal.OBJECITVE: To assess the systemic bone mineral density (BMD and the periodontal situation in postmenopausal women, to understand the possible role of osteoporosis as a risk factor for periodontal disease. METHODS: The sample was comprised of 47 postmenopausal women, divided into 3 groups: 14 patients with normal bones (G1, 17 with osteopenia (G2 and 16 patients with osteoporosis (G3. Data was obtained using bone mineral density (BMD, obtained by dual energy x-ray absorptiometry (DXA in the lumbar area (L1-L4. Periodontal condition was

Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs in mouse embryonic stem cells.

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Siem van der Laan

Full Text Available Pluripotency of embryonic stem cells (ESC is tightly regulated by a network of transcription factors among which the estrogen-related receptor β (Esrrb. Esrrb contributes to the relaxation of the G1 to S-phase (G1/S checkpoint in mouse ESCs by transcriptional control of the deubiquitylase Dub3 gene, contributing to Cdc25A persistence after DNA damage. We show that in mESCs, Dub3 gene expression is cell cycle regulated and is maximal prior G1/S transition. In addition, following UV-induced DNA damage in G1, Dub3 expression markedly increases in S-phase also suggesting a role in checkpoint recovery. Unexpectedly, we also observed cell cycle-regulation of Nanog expression, and not Oct4, reaching high levels prior to G1/S transition, finely mirroring Cyclin E1 fluctuations. Curiously, while Esrrb showed only limited cell-cycle oscillations, transcript levels of the p160 family of nuclear receptor coactivators (NCoAs displayed strong cell cycle-dependent fluctuations. Since NCoAs function in concert with Esrrb in transcriptional activation, we focussed on NCoA1 whose levels specifically increase prior onset of Dub3 transcription. Using a reporter assay, we show that NCoA1 potentiates Esrrb-mediated transcription of Dub3 and we present evidence of protein interaction between the SRC1 splice variant NCoA1 and Esrrb. Finally, we show a differential developmental regulation of all members of the p160 family during neural conversion of mESCs. These findings suggest that in mouse ESCs, changes in the relative concentration of a coactivator at a given cell cycle phase, may contribute to modulation of the transcriptional activity of the core transcription factors of the pluripotent network and be implicated in cell fate decisions upon onset of differentiation.

Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs) in mouse embryonic stem cells.

Science.gov (United States)

van der Laan, Siem; Golfetto, Eleonora; Vanacker, Jean-Marc; Maiorano, Domenico

2014-01-01

Pluripotency of embryonic stem cells (ESC) is tightly regulated by a network of transcription factors among which the estrogen-related receptor β (Esrrb). Esrrb contributes to the relaxation of the G1 to S-phase (G1/S) checkpoint in mouse ESCs by transcriptional control of the deubiquitylase Dub3 gene, contributing to Cdc25A persistence after DNA damage. We show that in mESCs, Dub3 gene expression is cell cycle regulated and is maximal prior G1/S transition. In addition, following UV-induced DNA damage in G1, Dub3 expression markedly increases in S-phase also suggesting a role in checkpoint recovery. Unexpectedly, we also observed cell cycle-regulation of Nanog expression, and not Oct4, reaching high levels prior to G1/S transition, finely mirroring Cyclin E1 fluctuations. Curiously, while Esrrb showed only limited cell-cycle oscillations, transcript levels of the p160 family of nuclear receptor coactivators (NCoAs) displayed strong cell cycle-dependent fluctuations. Since NCoAs function in concert with Esrrb in transcriptional activation, we focussed on NCoA1 whose levels specifically increase prior onset of Dub3 transcription. Using a reporter assay, we show that NCoA1 potentiates Esrrb-mediated transcription of Dub3 and we present evidence of protein interaction between the SRC1 splice variant NCoA1 and Esrrb. Finally, we show a differential developmental regulation of all members of the p160 family during neural conversion of mESCs. These findings suggest that in mouse ESCs, changes in the relative concentration of a coactivator at a given cell cycle phase, may contribute to modulation of the transcriptional activity of the core transcription factors of the pluripotent network and be implicated in cell fate decisions upon onset of differentiation.

Quality Assurance Project Plan for the HWMA/RCRA Closure Certification of the TRA-731 Caustic and Acid Storage Tank System - 1997 Notice of Violation Consent Order; TOPICAL

International Nuclear Information System (INIS)

Evans, S.K.

2002-01-01

This Quality Assurance Project Plan for the HWMA/RCRA Closure Certification of the TRA- 731 Caustic and Acid Storage Tank System is one of two documents that comprise the Sampling and Analysis Plan for the HWMA/RCRA closure certification of the TRA-731 caustic and acid storage tank system at the Idaho National Engineering and Environmental Laboratory. This plan, which provides information about the project description, project organization, and quality assurance and quality control procedures, is to be used in conjunction with the Field Sampling Plan for the HWMA/RCRA Closure Certification of the TRA-731 Caustic and Acid Storage Tank System. This Quality Assurance Project Plan specifies the procedures for obtaining the data of known quality required by the closure activities for the TRA-731 caustic and acid storage tank system

L’università italiana tra continuità e discontinuità. Intervista a Mauro Moretti

Directory of Open Access Journals (Sweden)

Alessandro Breccia

2017-07-01

Full Text Available L’intervista a Mauro Moretti inquadra il tema del dossier a partire dalle fonti per la storia delle università italiane nel secondo Novecento. Passa poi ad indicare alcuni tra i principali aspetti da indagare anche per definire al meglio una possibile cronologia

Alterações Ósseas e do Sistema Estomatognático associadas a Doenças Endócrino Metabólicas

OpenAIRE

Matos Paraguassú, Gardênia; Andrade Pimentel, Poliana; Sacramento dos Santos, Mércia; Morais Godoy Figueiredo, Leonardo; Pedreira Ramalho, Luciana Maria; Almeida Sarmento, Viviane

2013-01-01

Introdução: a prevalência de doenças endócrino-metabólicas tem aumentado nos últimos anos com a melhoria da expectativa de vida da população. Diabete Melittus, hipotireoidismo, hipertireoidismo, hipoparatireoidismo e hiperparatireoidismo podem levar as alterações no sistema estomatognático, contudo, a literatura ainda é limitada nesta área. Objetivo: Abordar as características e o processo fisiopatológico de doenças endócrino-metabólicas, com destaque para as alterações ósseas e do sistema es...

Massa óssea e composição corporal em estudantes universitários Bone mass and body composition in college students

Directory of Open Access Journals (Sweden)

Cristina Reuter

2012-06-01

Full Text Available OBJETIVO: Comparar a densidade mineral óssea (DMO e a composição corporal (CC de universitários com diferentes estilos de vida. MÉTODOS: Estudo transversal realizado em 85 estudantes dos cursos de Medicina (MED e Educação Física (EF da Universidade Regional de Blumenau. As variáveis antropométricas, sociodemográficas, clínicas e de estilo de vida foram obtidas por meio de anamnese densitométrica e as variáveis densitométricas por raio-x de dupla energia (DXA. Os testes estatísticos foram: t de Student, qui-quadrado e regressão logística. RESULTADOS: Os acadêmicos de EF apresentaram massa magra maior (79,5 ± 5,9 versus 75,1 ± 5,3; p = 0,03 e gordura corporal menor (16,7 ± 6,1 versus 21,6 ± 5,6; p = 0,02, e as acadêmicas de EF apresentaram massa magra maior (68,2 ± 5,5 versus 65,3 ± 5,5; p = 0,05. A DMO do colo do fêmur (CF, fêmur total (FT e corpo total (CT foi maior nos acadêmicos de EF em ambos os sexos. Os estudantes de EF praticavam mais exercícios físicos do que os de MED. A baixa massa óssea (BMO foi mais frequente nos estudantes de MED (34,9% versus 4,7%; p = 0,001, sendo que o risco de um estudante de MED ter BMO foi 9 vezes maior para a CL, 5 vezes para o CF, 8 vezes para o FT e 7 vezes para o CT. CONCLUSÃO: A CC e a DMO foram diferentes entre os estudantes; os acadêmicos de MED apresentaram um risco maior de ter BMO e os acadêmicos de EF praticavam mais exercícios físicos.OBJECTIVE: To compare bone mineral density (BMD and body composition (BC of college students with different lifestyles. METHODS: Transversal study with 85 students of Medicine (MED and Physical Education (PE at the Universidade Regional de Blumenau, SC, Brazil. The anthropometric, socio-demographic, clinical, and lifestyle variables were obtained through densitometric anamnesis and densitometric variables by dual-energy X-ray (DXA. The statistical tests used were: Student's t-test, Chi-square test, and logistic regression. RESULTS

pTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

Science.gov (United States)

Wagner, Sabine G; Ziegler, Martin; Löwe, Hannes; Kremling, Andreas; Pflüger-Grau, Katharina

2018-01-01

The presence of standardised tools and methods to measure and represent accurately biological parts and functions is a prerequisite for successful metabolic engineering and crucial to understand and predict the behaviour of synthetic genetic circuits. Many synthetic gene networks are based on transcriptional circuits, thus information on transcriptional and translational activity is important for understanding and fine-tuning the synthetic function. To this end, we have developed a toolkit to analyse systematically the transcriptional and translational activity of a specific synthetic part in vivo. It is based on the plasmid pTRA and allows the assignment of specific transcriptional and translational outputs to the gene(s) of interest (GOI) and to compare different genetic setups. By this, the optimal combination of transcriptional strength and translational activity can be identified. The design is tested in a case study using the gene encoding the fluorescent mCherry protein as GOI. We show the intracellular dynamics of mRNA and protein formation and discuss the potential and shortcomings of the pTRA plasmid.

Doença celíaca em tratamento: avaliação da densidade mineral óssea Celiac disease under treatment: evaluation of bone mineral density

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Cecília Noronha de Miranda Carvalho

2003-08-01

Full Text Available OBJETIVO: comparar a densidade mineral óssea de crianças e adolescentes com doença celíaca em tratamento com controles sadios, e avaliar exames laboratoriais relacionados com o metabolismo do cálcio. MÉTODOS: foram estudados 30 pacientes com doença celíaca em dieta isenta de glúten, 17 crianças e 13 adolescentes, e 23 indivíduos saudáveis. Todos os pacientes e controles realizaram a densidade mineral óssea (DEXA, Lunar. Os pacientes realizaram dosagem sérica de cálcio total, cálcio ionizado, fósforo, magnésio, fosfatase alcalina e paratormônio. RESULTADOS: a média de peso, estatura e densidade mineral óssea dos adolescentes com doença celíaca foi menor do que dos controles (pOBJECTIVE: the present study was designed to compare the bone mineral density of children and adolescents with celiac disease to the bone mineral density of controls, and to evaluate laboratory analysis of calcium metabolism of celiac disease patients. METHODS: thirty celiac disease patients (17 children, 13 adolescents, on a gluten-free diet, and 23 healthy subjects were studied. Tests of bone mineral density of the lumbar spine (DEXA, Lunar were performed in all patients and controls. Laboratory analysis of calcium metabolism was performed in all patients. RESULTS: mean weight and height of adolescents with celiac disease were lower than mean weight and height of controls (p<0.05. Bone mineral density in adolescents with celiac disease was significantly reduced if compared to controls (p=0.015, whereas no significant difference was found among children with celiac disease and controls. The number of adolescents who had started a gluten-free diet after the age of 2 years was higher than in children (p=0.003. Serum levels of ionized calcium, total calcium and parathormone were normal. CONCLUSIONS: the one mineral density of adolescents with celiac disease was lower than controls; whereas, no difference was found between the bone mineral density of

45 CFR 60.1 - The National Practitioner Data Bank.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false The National Practitioner Data Bank. 60.1 Section 60.1 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION NATIONAL PRACTITIONER DATA BANK FOR ADVERSE INFORMATION ON PHYSICIANS AND OTHER HEALTH CARE PRACTITIONERS General...

Anticonvulsant activity of a mGlu(4alpha) receptor selective agonist, (1S,3R,4S)-1-aminocyclopentane-1,2,4-tricarboxylic acid.

Science.gov (United States)

Chapman, A G; Talebi, A; Yip, P K; Meldrum, B S

2001-07-20

The metabotropic Group III agonist, (1S,3R,4S)-1-aminocyclopentane-1,2,4-tricarboxylic acid (ACPT-1), selective for the mGlu(4alpha) receptor, suppresses sound-induced seizures in DBA/2 mice following its intracerebroventricular (i.c.v.) administration (ED(50) 5.6 [2.9-10.7], nmol i.c.v., 15 min, clonic phase) and in genetically epilepsy-prone (GEP) rats following focal administration into the inferior colliculus (ED(50) 0.08 [0.01-0.50], nmol, 60 min, clonic phase). ACPT-1 also protects against clonic seizures induced in DBA/2 mice by the Group I agonist, (RS)-3,5-dihydroxyphenylglycine (3,5-DHPG) (ED(50) 0.60 [0.29-1.2], nmol i.c.v.) and by the Group III antagonist, (RS)-alpha-methylserine-O-phosphate (MSOP) (ED(50) 49.3 [37.9-64.1], nmol i.c.v.). Another Group III agonist, (RS)-4-phosphonophenyl-glycine (PPG), preferentially activating the mGlu(8) receptor, previously shown to protect against sound-induced seizures in DBA/2 mice and GEP rats, also protects against seizures induced in DBA/2 by 3,5-DHPG (ED(50) 3.7 [2.4-5.7], nmol i.c.v.) and by the Group III antagonist, MSOP (ED(50) 40.2 [21.0-77.0], nmol i.c.v.). At very high doses (500 nmol i.c.v. and above), Group III antagonists have pro-convulsant and convulsant activity. The anticonvulsant protection against sound-induced seizures in DBA/2 mice provided by a fully protective dose (20 nmol, i.c.v.) of the mGlu(4) receptor agonist ACPT-1, is partially reversed by the co-administration of the Group III antagonists, MSOP, (RS)-alpha-methyl-4-phosphonophenylglycine (MPPG) or (S)-2-amino-2-methyl-4-phosphonobutanoic acid (MAP4), in the 20-50 nmol dose range. At doses of 50-200 nmol, MPPG and MAP4 cause further reversal of the ACPT-1 anticonvulsant protection, while the MSOP effect on ACPT-1 protection is abolished at higher doses. In contrast, the anticonvulsant protection against sound-induced seizures in DBA/2 mice provided by a fully protective dose (20 nmol, i.c.v.) of the mGlu(8) receptor agonist PPG, is not

40 CFR 60.482-1a - Standards: General.

Science.gov (United States)

2010-07-01

... startup. (b) Compliance with §§ 60.482-1a to 60.482-10a will be determined by review of records and... in VOC service only during startup and shutdown, excluding startup and shutdown between batches of... 100...

Expression of the pluripotency transcription factor OCT4 in the normal and aberrant mammary gland

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Foteini eHassiotou

2013-04-01

Full Text Available Breast cancers with lactating features, some of which are associated with pregnancy and lactation, are often poorly differentiated, lack estrogen receptor, progesterone receptor and HER2 expression and have high mortality. Very little is known about the molecular mechanisms that drive uncontrolled cell proliferation in these tumors and confer lactating features. We have recently reported expression of OCT4 and associated embryonic stem cell (ESC self-renewal genes in the normal lactating breast and breastmilk stem cells (hBSCs. This prompted us to examine OCT4 expression in breast cancers with lactating features and compare it with that observed during normal lactation, using rare specimens of human lactating breast. In accordance with previous literature, the normal resting breast (from non-pregnant, non-lactating women showed minimal OCT4 nuclear expression (0.9%. However, this increased in the normal lactating breast (11.4%, with further increase in lactating adenomas, lactating carcinomas and pregnancy-associated breast cancer (30.7-48.3%. OCT4 was expressed in the epithelium and at lower levels in the stroma, and was co-localized with NANOG. Comparison of normal non-tumorigenic hBSCs with OCT4-overexpressing tumorigenic breast cell lines (OTBCs demonstrated upregulation of OCT4, SOX2 and NANOG in both systems, but OTBCs expressed OCT4 at significantly higher levels than SOX2 and NANOG. Similar to hBSCs, OTBCs displayed multi-lineage differentiation potential, including the ability to differentiate into functional lactocytes synthesizing milk proteins both in vitro and in vivo. Based on these findings, we propose a hypothesis of normal and malignant transformation in the breast, which centers on OCT4 and its associated gene network. Although minimal expression of these embryonic genes can be seen in the breast in its resting state throughout life, a controlled program of upregulation of this gene network may be a potential regulator of the

Farinha de algas marinhas (“Lithothamnium calcareum”) como suplemento mineral na cicatrização óssea de autoenxerto cortical em cães

OpenAIRE

Costa Neto, João Moreira da; Teixeira, Raquel Graça; Sá, Marcelo Jorge Cavalcanti de; Lima, Alessandra Estrela da Silva; Jacinto Aragão, Glauber Sergio; Teixeira, Marcelo Weinstein; Martins Filho, Emanoel Ferreira; Toríbio, Júlia Morena de Miranda Leão; Azevedo, Adílio Santos de

2010-01-01

Foi avaliada, em cães, a influência da farinha de algas marinhas (Lithothamnium calcareum) como suplemento mineral na cicatrização de falha óssea cortical reconstituída com autoenxerto cortical. Foram utilizados dez cães adultos, machos, sem raça definida, com peso entre 10 e 15kg. O enxerto, constituído de um bloco cilíndrico de osso cortical foi obtido da diáfise proximal da ulna, mediante ostectomia com trefina de oito milímetros de diâmetro. Igualmente criada...

Estudo da radiosensibilidade ao 60CO do Vibrio cholerae O1 incorporado em ostras Radiosensibility of Vibrio cholerae o1 incorporated in oysters, to 60CO

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Ivany R de Moraes

2000-02-01

Full Text Available OBJETIVO: Avaliar a eficiência da radiação ionizante por 60CO na eliminação de Vibrio cholerae O1, El Tor Ogawa, não-toxigênico, incorporados laboratorialmente em ostras vivas da espécie Crassostrea brasiliana. MÉTODO: Foram selecionadas amostras de ostras provenientes de Canan��ia (litoral sul de São Paulo, Brasil, as quais foram contaminadas com Vibrio cholerae e irradiadas com doses de 0,5 kGy e 1,0 kGy. RESULTADOS: Foram observadas diminuições significativas do número inicial do microrganismo indicado: de 3,4.10(7 para 10³ e 10², respectivamente. Os valores de D10 correspondentes foram de 0,173 a 0,235. CONCLUSÃO: Adotando-se o fator 6 como nível de segurança, conclui-se que a dose de irradiação de 1,41 kGy é necessária para eliminar números elevados de células viáveis de V. cholerae em ostras. Os experimentos foram realizados com os controles respectivos.OBJECTIVE: Evaluate the effect of ionizing irradiation by 60Co on Vibrio cholerae O1, El-Tor, Ogawa, non-toxigenic, incorporated in live oysters Crassostrea brasiliana. METHODS: Samples of oysters were selected from Cananéia town in the South coast of S. Paulo state, Brazil, contaminated with Vibrio cholerae and irradiated with 60Co at 0.5 and 1.0 kGy dosages. RESULTS: Showed significant reductions of the initial number of V. cholerae , ranging from 3.4 x10(7 to 10³ and 10², respectively. The D10 values related with the respective doses of irradiation were 0.173 and 0.235. CONCLUSION: Considering a 6 value as safety factor, it is concluded that 1.41 kGy irradiation dosage is necessary to eliminate a high number of V. cholerae viable cells in oysters. Controls were used in the experiment.

Generalizzazione cartografica tra ricerca e applicazione La Cartografia Tecnica Regionale delle Marche

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Stefano Bellesi

2007-03-01

Full Text Available Il problema della generalizzazione cartografica è uno dei temi principe nel dibattito cartografico tra gli addetti ai lavori. La cartografia multiscala rappresenta infatti una delle chimere dell’era digitale e, man mano che i DB cartografici si sono popolati di informazioni, ha dimostrato la sua realtà. L’orientamento è quello di automatizzare al massimo tutte le procedure prendendo in considerazione quelle che sono le esigenze della rappresentazione.

Densidade mineral óssea, função pulmonar, idade cronológica e idade de diagnóstico em crianças e adolescentes com fibrose cística Bone mineral density, pulmonary function, chronological age, and age at diagnosis in children and adolescents with cystic fibrosis

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Márcio V.F. Donadio

2013-04-01

Full Text Available OBJETIVO: Avaliar a densidade mineral óssea de pacientes com fibrose cística (FC e correlacioná-la com possíveis variáveis intervenientes. MÉTODOS: Foram incluídos crianças e adolescentes com diagnóstico clínico de FC, idade entre seis e dezoito anos, e em acompanhamento ambulatorial. Primeiramente, foram coletados os dados demográficos, para posterior realização do teste espirométrico. Todos os pacientes responderam ao questionário de qualidade de vida em FC (QFC e realizaram o teste de caminhada dos seis minutos (TC6 e o exame de densitometria óssea (DXA. RESULTADOS: Foram incluídos 25 pacientes fibrocísticos, sendo 56% do sexo masculino. A média de idade foi de 12,3 ± 3,4 anos, altura de 149,2 ± 14,4 cm e peso de 44,4 ± 13,9 kg. A maioria dos dados de função pulmonar e de densidade mineral óssea (DMO encontrou-se dentro dos limites de normalidade. A média do volume expiratório forçado no primeiro segundo (VEF1 foi de 92,5 ± 23,6 (% do previsto, capacidade vital forçada (CVF de 104,4 ± 21,3 (% do previsto e o escore z da DMO de 0,1 ± 1,0. A DMO correlacionou-se de forma moderada com o VEF1 (r = 0,43; p = 0,03 e com a CVF (r = 0,57; p = 0,003. Em relação à idade cronológica e à idade de diagnóstico, também foi encontrada uma correlação moderada e inversa (r = -0,55; p = 0,004 /r = -0,57; p = 0,003, respectivamente. Entretanto, não foram encontradas correlações significativas com os dados do QFC, TC6 e índice de massa corporal. CONCLUSÃO: A maioria dos pacientes avaliados apresenta DMO dentro dos limites de normalidade e possui correlação positiva com a função pulmonar e negativa com a idade cronológica e a idade de diagnóstico.OBJECTIVE: To assess bone mineral density in patients with cystic fibrosis (CF, and to correlate it with possible intervening variables. METHODS: Children and adolescents diagnosed with CF, aged 6 to 18 years, followed at the outpatient clinic were included in the study

Simulation of "6"0Co and "1"3"7Csspectra of irradiator Shepherd 81 - 14D do LNMRI/IRD using Geant4

International Nuclear Information System (INIS)

Queiroz Filho, P.P.; Cabral, T.S.; Silva, C.N.M. da

2016-01-01

In this work it were simulated, using Geant4, the "6"0Co and "1"3"7Cs spectra of the Irradiator Shepherd 81-14D acquired by the National Metrology Laboratory of Ionising Radiation of the Institute of Radiation Protection and Dosimetry ( LNMRI / IRD ), which will be used for calibration in radioprotection. Was checked if photons suffered his first interaction on the source, the structure of the head or the collimators. It was also observed the percentage of occurrence of each type of interaction in such structures. (author)

Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth

International Nuclear Information System (INIS)

Varga, Nóra; Veréb, Zoltán; Rajnavölgyi, Éva; Német, Katalin; Uher, Ferenc; Sarkadi, Balázs; Apáti, Ágota

2011-01-01

Highlights: ► MSC like cells were derived from hESC by a simple and reproducible method. ► Differentiation and immunosuppressive features of MSCl cells were similar to bmMSC. ► MSCl cells as feeder cells support the undifferentiated growth of hESC. -- Abstract: Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.

Very small embryonic-like stem cells: implications in reproductive biology.

Science.gov (United States)

Bhartiya, Deepa; Unni, Sreepoorna; Parte, Seema; Anand, Sandhya

2013-01-01

The most primitive germ cells in adult mammalian testis are the spermatogonial stem cells (SSCs) whereas primordial follicles (PFs) are considered the fundamental functional unit in ovary. However, this central dogma has recently been modified with the identification of a novel population of very small embryonic-like stem cells (VSELs) in the adult mammalian gonads. These stem cells are more primitive to SSCs and are also implicated during postnatal ovarian neo-oogenesis and primordial follicle assembly. VSELs are pluripotent in nature and characterized by nuclear Oct-4A, cell surface SSEA-4, and other pluripotent markers like Nanog, Sox2, and TERT. VSELs are considered to be the descendants of epiblast stem cells and possibly the primordial germ cells that persist into adulthood and undergo asymmetric cell division to replenish the gonadal germ cells throughout life. Elucidation of their role during infertility, endometrial repair, superovulation, and pathogenesis of various reproductive diseases like PCOS, endometriosis, cancer, and so on needs to be addressed. Hence, a detailed review of current understanding of VSEL biology is pertinent, which will hopefully open up new avenues for research to better understand various reproductive processes and cancers. It will also be relevant for future regenerative medicine, translational research, and clinical applications in human reproduction.

Very Small Embryonic-Like Stem Cells: Implications in Reproductive Biology

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Deepa Bhartiya

2013-01-01

Full Text Available The most primitive germ cells in adult mammalian testis are the spermatogonial stem cells (SSCs whereas primordial follicles (PFs are considered the fundamental functional unit in ovary. However, this central dogma has recently been modified with the identification of a novel population of very small embryonic-like stem cells (VSELs in the adult mammalian gonads. These stem cells are more primitive to SSCs and are also implicated during postnatal ovarian neo-oogenesis and primordial follicle assembly. VSELs are pluripotent in nature and characterized by nuclear Oct-4A, cell surface SSEA-4, and other pluripotent markers like Nanog, Sox2, and TERT. VSELs are considered to be the descendants of epiblast stem cells and possibly the primordial germ cells that persist into adulthood and undergo asymmetric cell division to replenish the gonadal germ cells throughout life. Elucidation of their role during infertility, endometrial repair, superovulation, and pathogenesis of various reproductive diseases like PCOS, endometriosis, cancer, and so on needs to be addressed. Hence, a detailed review of current understanding of VSEL biology is pertinent, which will hopefully open up new avenues for research to better understand various reproductive processes and cancers. It will also be relevant for future regenerative medicine, translational research, and clinical applications in human reproduction.

Simulation of a small computer of the TRA-1001 type on the BESM computer

International Nuclear Information System (INIS)

Galaktionov, V.V.

1975-01-01

Considered are the purpose and probable simulation ways of one computer by the other. The emulator (simulation program) is given for a small computer of TRA-1001 type on BESM-6 computer. The simulated computer basic elements are the following: memory (8 K words), central processor, input-output program channel, interruption circuit, computer panel. The work with the input-output devices, teletypes ASP-33, FS-1500 is also simulated. Under actual operation the emulator has been used for translating the programs prepared on punched cards with the aid of translator SLANG-1 by BESM-6 computer. The translator alignment from language COPLAN has been realized with the aid of the emulator

Autologous mononuclear bone marrow cells in the regeneration of tibial nerve of rabbits submitted to neurectomy: morphofunctional aspectsCélulas mononucleares autólogas de medula óssea na regeneração do nervo tibial de coelhos submetidos à neurectomia: aspectos morfofuncionais

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Camila França de Paula Orlando-Goulart

2013-10-01

Full Text Available The functional recovery after peripheral nerve injury is the main goal of therapeutic intervention. Therefore the aim of this study was to clinically evaluate functional recovery in rabbits after neurectomy and treatment with bone marrow mononuclear cells associated with the tubulization technique. For this, 24 New Zealand rabbits, were used. They were divided into two groups with 12 animals each, mononuclear cell group (MCG and saline group (SSG. The rabbits underwent right tibial nerve section and repair by the tubulization technique using silicone hollow tube, which received 0.1 ml of autologous mononuclear bone marrow cells (2 x 106 cells in the MCG and of saline solution in the SSG, in order to compare the evolution of functional recovery of the operated limbs. Gait and planimetry were performed. Planimetry of the pelvic limb footprint printed on paper with water-based ink applied to the plantar area, before (M0 and after 7 (M7, 15 (M15, 30 (M30, 45 (M45 and 60 (M60 days after surgery. The results showed no functional recovery of the tibial nerve in both groups, without differences between them in different times and among times within groups, except when compared to M0.A recuperação funcional de nervos periféricos após lesão é o principal objetivo da intervenção terapêutica. Por isso o objetivo deste estudo foi avaliar clinicamente a recuperação funcional de coelhos após neurectomia e tratamento com células mononucleares de medula óssea associada à técnica de tubulização. Foram utilizados para isto, 24 coelhos da raça Nova Zelândia, alocados em dois grupos com 12 animais cada, denominados grupo célula mononuclear (GCM e grupo solução salina (GSS. Os coelhos foram submetidos à secção do nervo tibial direito e reparação por meio da técnica de tubulização utilizando tubo oco de silicone, para então, receberem no interior do tubo, 0,1mL de suspensão de células mononucleares autólogas de medula óssea (2 x 106

Derivation and characterization of human fetal MSCs: an alternative cell source for large-scale production of cardioprotective microparticles.

Science.gov (United States)

Lai, Ruenn Chai; Arslan, Fatih; Tan, Soon Sim; Tan, Betty; Choo, Andre; Lee, May May; Chen, Tian Sheng; Teh, Bao Ju; Eng, John Kun Long; Sidik, Harwin; Tanavde, Vivek; Hwang, Wei Sek; Lee, Chuen Neng; El Oakley, Reida Menshawe; Pasterkamp, Gerard; de Kleijn, Dominique P V; Tan, Kok Hian; Lim, Sai Kiang

2010-06-01

The therapeutic effects of mesenchymal stem cells (MSCs) transplantation are increasingly thought to be mediated by MSC secretion. We have previously demonstrated that human ESC-derived MSCs (hESC-MSCs) produce cardioprotective microparticles in pig model of myocardial ischemia/reperfusion (MI/R) injury. As the safety and availability of clinical grade human ESCs remain a concern, MSCs from fetal tissue sources were evaluated as alternatives. Here we derived five MSC cultures from limb, kidney and liver tissues of three first trimester aborted fetuses and like our previously described hESC-derived MSCs; they were highly expandable and had similar telomerase activities. Each line has the potential to generate at least 10(16-19) cells or 10(7-10) doses of cardioprotective secretion for a pig model of MI/R injury. Unlike previously described fetal MSCs, they did not express pluripotency-associated markers such as Oct4, Nanog or Tra1-60. They displayed a typical MSC surface antigen profile and differentiated into adipocytes, osteocytes and chondrocytes in vitro. Global gene expression analysis by microarray and qRT-PCR revealed a typical MSC gene expression profile that was highly correlated among the five fetal MSC cultures and with that of hESC-MSCs (r(2)>0.90). Like hESC-MSCs, they produced secretion that was cardioprotective in a mouse model of MI/R injury. HPLC analysis of the secretion revealed the presence of a population of microparticles with a hydrodynamic radius of 50-65 nm. This purified population of microparticles was cardioprotective at approximately 1/10 dosage of the crude secretion. (c) 2009 Elsevier Ltd. All rights reserved.

CDC 1604-A translator from the SLANG autocode for the TRA computer

International Nuclear Information System (INIS)

Belyaev, A.V.

1976-01-01

A SLANG - TRA translator has been devised for faster, easier programing. The program is realized on a SDS-1604A computer, input data are read from 80 column punch cards and translated into the standard Hollerith 026 code. Programs are processed in batches. A SDS-1604A teletype enables the operator to control the translation. The translator makes it possible to evaluate program processing time. The translator's high speed simplifies program editing and saves manpower

A model-based analysis of culture-dependent phenotypes of mESCs.

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Maria Herberg

Full Text Available Mouse embryonic stem cells (mESCs can be maintained in a proliferative and undifferentiated state over many passages (self-renewal while retaining the potential to give rise to every cell type of the organism (pluripotency. Autocrine FGF4/Erk signalling has been identified as a major stimulus for fate decisions and lineage commitment in these cells. Recent findings on serum-free culture conditions with specific inhibitors (known as 2i demonstrate that the inhibition of this pathway reduces transcription factor heterogeneity and is vital to maintain ground state pluripotency of mESCs. We suggest a novel mathematical model to explicitly integrate FGF4/Erk signalling into an interaction network of key pluripotency factors (namely Oct4, Sox2, Nanog and Rex1. The envisaged model allows to explore whether and how proposed mechanisms and feedback regulations can account for different expression patterns in mESC cultures. We demonstrate that an FGF4/Erk-mediated negative feedback is sufficient to induce molecular heterogeneity with respect to Nanog and Rex1 expression and thus critically regulates the propensity for differentiation and the loss of pluripotency. Furthermore, we compare simulation results on the transcription factor dynamics in different self-renewing states and during differentiation with experimental data on a Rex1GFPd2 reporter cell line using flow cytometry and qRT-PCR measurements. Concluding from our results we argue that interaction between FGF4/Erk signalling and Nanog expression qualifies as a key mechanism to manipulate mESC pluripotency. In particular, we infer that ground state pluripotency under 2i is achieved by shifting stable expression pattern of Nanog from a bistable into a monostable regulation impeding stochastic state transitions. Furthermore, we derive testable predictions on altering the degree of Nanog heterogeneity and on the frequency of state transitions in LIF/serum conditions to challenge our model assumptions.

Comparação de dois modelos de treinamento de força na densidade mineral óssea, força muscular, antropometria e lesão muscular em mulheres pré-menopáusicas

OpenAIRE

Adriane Carla Vanni

2008-01-01

O objetivo geral deste estudo foi avaliar e comparar os efeitos de dois modelos de periodização de treinamento de força na densidade mineral óssea (DMO), na força muscular dinâmica máxima (1-RM) e submáxima (20-RM), parâmetros de lesão muscular e parâmetros antropométricos de mulheres na prémenopausa. Vinte e sete mulheres, sem osteopenia ou osteoporose, foram divididas aleatoriamente em dois grupos experimentais: (1) treinamento de força com periodização linear (GPL – n=14), com intensidades...

Dosimetric intercomparison in Cobalt 60 unities using TLD-100 crystals and CaSO4: Dy + Ptfe

International Nuclear Information System (INIS)

Gaona, E.; Azorin N, J.; Perez P, M.A.; Castillo H, M.; Flores F, F.; Guzman R, L.V.

1999-01-01

The purpose of this work is to design and construct a phantom in basis of thermoplastic polymer of methyl methacrylate that is reutilizable and which allows to do an analysis of the absorbed doses in thermoluminescent crystals (Tl) exposed to cobalt 60 to establish the dosimetric intercomparison in cobalt units which allows to do the follow-up of the Quality assurance programs, standardization of calibration procedures, dosimetry and TLD post intercomparison in radiotherapy. This work allows also prove new thermoluminescent materials of national manufacture developed by Juan Azorin and collaborators as the CaSO 4 : Dy + Ptfe. This is a first study which is realized in Mexico with the system crystal-phantom for aims to intercomparison in cobalt 60 units. In this work participate eight unities of cobalt 60 of different trades belonging at four radiotherapy centers. The results of the dose intercomparison of the eight unities of cobalt 60 were in the range 0.95-1.13, taking in account that the values between 0.95 and 1.05 were considered acceptable in terms of the requirements by the standing legislation. (Author)

Nuclear Effects Analysis D1-S-1800 Aerial Radiac System AN/ADR-6(XE-4) (V).

Science.gov (United States)

1972-12-21

1 ,r-4»-!4.. l.F-4,l&#60»..l .«14. TAP |. FT = 0.n.c;,OF-A.n.c.oiF-nft . 1 . OF 8 « 7 . 4<^P-* , 1 . OF« . 7 . SF-6 • 0 * 1 .F-2,0 TAPI[ F4...CffC49\\A|f r* if p- or &#60 ( eft R &#60\\r\\r^f^OftCC~~ ’v.fvtvrv.fv — rv^. •* i i i i I i njjliflf. -ceo cccoccccc I I I I I I

The H_6_0Si_6C_5_4 heterofullerene as high-capacity hydrogen storage medium

International Nuclear Information System (INIS)

Yong, Yongliang; Zhou, Qingxiao; Li, Xiaohong; Lv, Shijie

2016-01-01

With the great success in Si atoms doped C_6_0 fullerene and the well-established methods for synthesis of hydrogenated carbon fullerenes, this leads naturally to wonder whether Si-doped fullerenes are possible for special applications such as hydrogen storage. Here by using first-principles calculations, we design a novel high-capacity hydrogen storage material, H_6_0Si_6C_5_4 heterofullerene, and confirm its geometric stability. It is found that the H_6_0Si_6C_5_4 heterofullerene has a large HOMO-LUMO gap and a high symmetry, indicating it is high chemically stable. Further, our finite temperature simulations indicate that the H_6_0Si_6C_5_4 heterofullerene is thermally stable at 300 K. H_2 molecules would enter into the cage from the Si-hexagon ring because of lower energy barrier. Through our calculation, a maximum of 21 H_2 molecules can be stored inside the H_6_0Si_6C_5_4 cage in molecular form, leading to a gravimetric density of 11.11 wt% for 21H_2@H_6_0Si_6C_5_4 system, which suggests that the hydrogenated Si_6C_5_4 heterofullerene could be suitable as a high-capacity hydrogen storage material.

Una community emergente tra progettazione territorio e infrastrutture La prima conferenza Autodesk degli utenti italiani

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Redazione Redazione

2005-10-01

Full Text Available La prima edizione dell’Autodesk User Conference dedicata aiSistemi Informativi Territoriali ed alla progettazione delle infrastrutture, tenutasi a Roma il 29 settembre 2005, diventerà l’appuntamento fisso sulla convergenza tra tecnologie CAD e GISche molti reclamavano da tempo.

La narrazione orale tra bambini ed adulti: da flusso continuo a vena carsica?

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Milena Bernardi

2010-02-01

Full Text Available Con il segmento di ricerca che viene presentato nella sua prima fase si prende in esame l’ipotesi di un processo, in atto da tempo, di trasformazione e/o di declino della narrazione orale nel rapporto quotidiano tra adulti e bambini, inteso, in questo specifico contesto, come “spia” dei mutamenti degli stili educativi e degli interscambi tra famiglie ed operatori dei servizi in relazione all’alleanza educativa. La complessità dei filoni di studio che rimandano alla voce “narrazione” negli ambiti delle scienze umane esige la precisazione della curvatura “narrativa” scelta per la ricerca in corso: curvatura che appartiene all’epistemologia della letteratura per l’infanzia e della pedagogia della narrazione in quanto permette di imboccare la strada della ricerca pedagogica rivolta innanzi tutto ai significati che il narrare assume nella relazione educativa e poi ai modi ed alle forme della narrazione che coinvolgono l’infanzia. In un’epoca di grande vitalità e visibilità delle narrazioni, tra cui abbondano le produzioni scritte, illustrate, filmate per l’infanzia, sembra esserci una zona marginale e quasi sommersa della comunicazione narrante che rischia di sfumare in una sorta di oblio. E’ la zona della narrazione orale esperita in praesentia da bambini ed adulti insieme, tessuta con fili di parole che restituiscono senso all’intero arco delle esperienze, tentando di riordinare il disordine che spesso connota la realtà, interpretando, stabilendo connessioni, impregnando di quel senso i vissuti, i fatti, le relazioni, le cose e le parole stesse, ancora, nelle voci. Del rischio e delle conseguenze che possono derivare dalla sua mancanza si occupa questo contributo di ricerca che, dal punto di vista metodologico, si è avvalso e si avvale sia di analisi condotte sul corpus dei testi sia della raccolta di dati e informazioni tramite incontri condotti con la modalità del focus group, con educatrici e genitori.

17 CFR 1.60 - Pending legal proceedings.

Science.gov (United States)

2010-04-01

... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Pending legal proceedings. 1... REGULATIONS UNDER THE COMMODITY EXCHANGE ACT Miscellaneous § 1.60 Pending legal proceedings. (a) Every... section to be submitted to the Commission as to matters pending on the effective date of the section (May...

4-[(3-Hydroxyanilino(phenylmethylidene]-3-methyl-1-phenyl-1H-pyrazol-5(4H-one

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Keraghel Saida

2012-06-01

Full Text Available In the title compound, C23H19N3O2, the dihedral angles formed by the pyrazolone ring with the three benzene rings are 30.91 (6, 60.96 (4 and 57.01 (4°. The ligand is in the enamine–keto form and its structure is stabilized by an intramolecular N—H...O hydrogen bond. In the crystal, O—H...N hydrogen bonds link molecules into chains parallel to [01-1].

Metronomic chemotherapy in the neoadjuvant setting: results of two parallel feasibility trials (TraQme and TAME) in patients with HER2+ and HER2− locally advanced breast cancer

International Nuclear Information System (INIS)

Petry, V.; Gagliato, D.M.; Leal, A.I.C.; Arai, R.J.; Longo, E.; Andrade, F.; Ricci, M.D.; Piato, J.R.; Barroso-Sousa, R.; Hoff, P.M.; Mano, M.S.

2015-01-01

Neoadjuvant chemotherapy has practical and theoretical advantages over adjuvant chemotherapy strategy in breast cancer (BC) management. Moreover, metronomic delivery has a more favorable toxicity profile. The present study examined the feasibility of neoadjuvant metronomic chemotherapy in two cohorts [HER2+ (TraQme) and HER2− (TAME)] of locally advanced BC. Twenty patients were prospectively enrolled (TraQme, n=9; TAME, n=11). Both cohorts received weekly paclitaxel at 100 mg/m 2 during 8 weeks followed by weekly doxorubicin at 24 mg/m 2 for 9 weeks in combination with oral cyclophosphamide at 100 mg/day (fixed dose). The HER2+ cohort received weekly trastuzumab. The study was interrupted because of safety issues. Thirty-six percent of patients in the TAME cohort and all patients from the TraQme cohort had stage III BC. Of note, 33% from the TraQme cohort and 66% from the TAME cohort displayed hormone receptor positivity in tumor tissue. The pathological complete response rates were 55% and 18% among patients enrolled in the TraQme and TAME cohorts, respectively. Patients in the TraQme cohort had more advanced BC stages at diagnosis, higher-grade pathological classification, and more tumors lacking hormone receptor expression, compared to the TAME cohort. The toxicity profile was also different. Two patients in the TraQme cohort developed pneumonitis, and in the TAME cohort we observed more hematological toxicity and hand-foot syndrome. The neoadjuvant metronomic chemotherapy regimen evaluated in this trial was highly effective in achieving a tumor response, especially in the HER2+ cohort. Pneumonitis was a serious, unexpected adverse event observed in this group. Further larger and randomized trials are warranted to evaluate the association between metronomic chemotherapy and trastuzumab treatment

Metronomic chemotherapy in the neoadjuvant setting: results of two parallel feasibility trials (TraQme and TAME) in patients with HER2+ and HER2− locally advanced breast cancer

Energy Technology Data Exchange (ETDEWEB)

Petry, V.; Gagliato, D.M.; Leal, A.I.C.; Arai, R.J.; Longo, E. [Divisão de Oncologia Médica, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP (Brazil); Andrade, F. [Núcleo de Mastologia, Hospital Sírio Libanês, São Paulo, SP (Brazil); Ricci, M.D.; Piato, J.R.; Barroso-Sousa, R.; Hoff, P.M.; Mano, M.S. [Divisão de Oncologia Médica, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP (Brazil)

2015-03-06

Neoadjuvant chemotherapy has practical and theoretical advantages over adjuvant chemotherapy strategy in breast cancer (BC) management. Moreover, metronomic delivery has a more favorable toxicity profile. The present study examined the feasibility of neoadjuvant metronomic chemotherapy in two cohorts [HER2+ (TraQme) and HER2− (TAME)] of locally advanced BC. Twenty patients were prospectively enrolled (TraQme, n=9; TAME, n=11). Both cohorts received weekly paclitaxel at 100 mg/m{sup 2} during 8 weeks followed by weekly doxorubicin at 24 mg/m{sup 2} for 9 weeks in combination with oral cyclophosphamide at 100 mg/day (fixed dose). The HER2+ cohort received weekly trastuzumab. The study was interrupted because of safety issues. Thirty-six percent of patients in the TAME cohort and all patients from the TraQme cohort had stage III BC. Of note, 33% from the TraQme cohort and 66% from the TAME cohort displayed hormone receptor positivity in tumor tissue. The pathological complete response rates were 55% and 18% among patients enrolled in the TraQme and TAME cohorts, respectively. Patients in the TraQme cohort had more advanced BC stages at diagnosis, higher-grade pathological classification, and more tumors lacking hormone receptor expression, compared to the TAME cohort. The toxicity profile was also different. Two patients in the TraQme cohort developed pneumonitis, and in the TAME cohort we observed more hematological toxicity and hand-foot syndrome. The neoadjuvant metronomic chemotherapy regimen evaluated in this trial was highly effective in achieving a tumor response, especially in the HER2+ cohort. Pneumonitis was a serious, unexpected adverse event observed in this group. Further larger and randomized trials are warranted to evaluate the association between metronomic chemotherapy and trastuzumab treatment.

Design, synthesis and anticonvulsant activity evaluation of 7-substituted-4H-[1,2,4]triazino[3,4-alpha]phthalazin-4-one derivatives

Energy Technology Data Exchange (ETDEWEB)

Sun, Xian-Yu; Quan, Zhe-Shan [Yanbian Univ., Yanji, Jilin (China). Key Lab. of Organism Functional Factors of the Changbai Mountain; Yanbian Univ., Yanji, Jilin (China). Coll. of Pharmacy; Guan, Li-Ping; Zhang, Lei; Wei, Cheng-Xi; Piao, Hu-Ri [Yanbian Univ., Yanji, Jilin (China). Key Lab. of Organism Functional Factors of the Changbai Mountain

2009-07-01

In this study, a novel series of 7-substituted-4H-[1,2,4]triazino[3,4-a]phthalazin-4-one derivatives was synthesized as potential anticonvulsant agents. Their anticonvulsant activities were evaluated by the maximal electroshock (MES) test, and their neurotoxicities were evaluated by the rotarod neurotoxicity test. The pharmacological results showed that 7-hexyloxy-4H-[1,2,4]triazino[3,4-alpha]phthalazin-4-one 4e was the most potent with median effective dose (ED{sub 50}) value of 6.6 mg kg-1, median toxicity dose (TD{sub 50}) of 39.4 mg kg{sup -1}, providing a protective index (PI=TD{sub 50} /ED{sub 50}) value of 6.0. (author)

Efeitos do alcoolismo e da desintoxicação alcoólica sobre o reparo e biomecânica óssea Effects of alcoholism and alcoholic detoxication on the repair and biomechanics of bone

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Renato de Oliveira Horvath

2011-01-01

Full Text Available OBJETIVO: Avaliar os efeitos do consumo crônico de etanol e da desintoxicação alcoólica sobre a resistência mecânica do osso e neoformação óssea junto a implantes de hidroxiapatita densa (HAD realizados em ratos. MÉTODOS: Foram utilizados 15 ratos divididos em três grupos, sendo controle (CT, alcoolista crônico (AC e desintoxicado (DE. Após quatro semanas, foi realizada implantação de HAD na tíbia e produzida falha no osso parietal, em seguida o grupo AC continuaram a consumir etanol e o grupo DE iniciaram a desintoxicação. Ao completar 13 semanas os animais sofreram eutanásia, os ossos foram coletados para o processamento histomorfométrico e os fêmures encaminhados ao teste mecânico de resistência. RESULTADOS: Os animais do grupo AC apresentaram menores valores de neoformação óssea, de calcemia e resistência mecânica, quando comparado aos grupos CT e DE. Os animais dos grupos DE apresentaram valores superiores em todas as variáveis avaliadas em relação ao grupo AC. CONCLUSÃO: O consumo de etanol interferiu na osteogênese ao redor de implante de HAD, nos níveis de cálcio e na resistência mecânica óssea. A desintoxicação alcoólica se mostrou eficaz, pois aumentou à osteogênese e osseointegração da HAD, a calcemia e resistência mecânica óssea. Nivel de Evidência I, estudo terapeutico.OBJECTIVE: To evaluate the effects of chronic ethanol consumption and alcohol detoxication on the mechanical resistance of bone and bone neoformation around dense hydroxyapatite implants (DHA in rats. METHODS: Fifteen rats were separated into three groups: (1 control group (CT; (2 chronic alcoholic (CA, and (3 disintoxicated (DI. After four weeks, a DHA was implanted in the right tibia of the animals, and the CA group continued consuming ethanol, while the DI group started detoxication. The solid and liquid feeding of the animals was recorded, and a new alcohol dilution was effected every 48 hours. After 13 weeks, the

New Mononuclear Cu(II Complexes and 1D Chains with 4-Amino-4H-1,2,4-triazole

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Marinela M. Dîrtu

2013-12-01

Full Text Available The crystal structures of two mononuclear Cu(II NH2trz complexes [Cu(NH2trz4(H2O](AsF62 (I and [Cu(NH2trz4(H2O](PF62 (II as well as two coordination polymers [Cu(μ2-NH2trz2Cl]Cl∙H2O (III and [Cu(μ2-NH2trz2Cl] (SiF60.5∙1.5H2O (IV are presented. Cationic 1D chains with bridging bis-monodentate μ2-coordinated NH2trz and bridging μ2-coordinated chloride ligands are present in III and IV. In these coordination polymers, the Cu(II ions are strongly antiferromagnetically coupled with J = −128.4 cm−1 for III and J = −143 cm−1 for IV (H = −JΣSiSi+1, due to the nature of the bridges between spin centers. Inter-chain interactions present in the crystal structures were taken into consideration, as well as g factors, which were determined experimentally, for the quantitative modeling of their magnetic properties.

Seleção de doador de medula óssea ou sangue periférico Bone marrow or peripheral blood donor selection

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Noemi F. Pereira

2010-05-01

Full Text Available A compatibilidade HLA é o fator mais valorizado na escolha do doador de medula óssea voluntário, preconizando-se a realização de HLA de alta resolução nos locos HLA-A,B,C, DRB1 e DQB1. Tem sido dado preferência para o doador com consanguinidade alélica 8x8 (A,B,C, DRB1. Na presença de incompatibilidade na classe-I sugere-se a busca de doador com compatibilidade DQB1 (9x10. Já as incompatibilidades dos locos DPB1 não constituem critério de exclusão de doador, exceto quando existir presença de anticorpo contra o loco HLA-DP do doador.The HLA system is considered the most important factor in choosing a volunteer bone marrow donor with the recommendation of performing high resolution HLA tests for the HLA-A, B, C, DRB1 and DQB1 loci. A preference has been given for donor 8x8 (A, B, C, DRB1 allele matching. In the presence of class-I incompatibility a search for DQB1 (9x10 donor compatibility is suggested. The incompatibility of the DPB1 locus does not constitute exclusion of the donor, except when there is the presence of antibodies against the HLA-DP locus of the donor.

Dosimetric intercomparison in Cobalt 60 unities using TLD-100 crystals and CaSO{sub 4}: Dy + Ptfe; Intercomparacion dosimetrica en unidades de cobalto 60 usando cristales de TLD-100 y CaSO{sub 4}: Dy + PTFE

Energy Technology Data Exchange (ETDEWEB)

Gaona, E. [Universidad Autonoma Metropolitana Xochimilco, Mexico D.F. (Mexico); Azorin N, J. [Universidad Autonoma Metropolitana Iztapalapa, Mexico D.F. (Mexico); Perez P, M.A. [Instituto Nacional de Cancerologia, Mexico D.F. (Mexico); Castillo H, M. [Centro Medico Nacional 20 de Noviembre, ISSSTE, Mexico D.F. (Mexico); Flores F, F. [Unidad de Oncologia, Centro Medico Nacional, IMSS, Mexico D.F. (Mexico); Guzman R, L.V. [Unidad de Oncologia, Hospital General de Mexico, Mexico D.F. (Mexico)

1999-07-01

The purpose of this work is to design and construct a phantom in basis of thermoplastic polymer of methyl methacrylate that is reutilizable and which allows to do an analysis of the absorbed doses in thermoluminescent crystals (Tl) exposed to cobalt 60 to establish the dosimetric intercomparison in cobalt units which allows to do the follow-up of the Quality assurance programs, standardization of calibration procedures, dosimetry and TLD post intercomparison in radiotherapy. This work allows also prove new thermoluminescent materials of national manufacture developed by Juan Azorin and collaborators as the CaSO{sub 4}: Dy + Ptfe. This is a first study which is realized in Mexico with the system crystal-phantom for aims to intercomparison in cobalt 60 units. In this work participate eight unities of cobalt 60 of different trades belonging at four radiotherapy centers. The results of the dose intercomparison of the eight unities of cobalt 60 were in the range 0.95-1.13, taking in account that the values between 0.95 and 1.05 were considered acceptable in terms of the requirements by the standing legislation. (Author)

Third-order nonlinearities and structural features in Langmuir-Blodgett films of 1-benzyl-9-hydrofullerene-60

International Nuclear Information System (INIS)

Shihong Ma; Liying Liu; Xingze Lu

1995-01-01

Third-order nonlinear susceptibilities χ xxxx (3) (-3ω; ω, ω, ω) have been deduced by measuring third-harmonic generation in Langmuir-Blodgett (LB) films of 1-benzyl-9-hydrofullerene-60 (C 60 -Be). The structural features of the condensed layer at the air-water interface and LB films of the C 60 -Be were investigated by small angle x-ray diffraction (SAXD) and optical measurements. The third-order nonlinear susceptibilities (χ (3) ) were obtained by measuring the THG intensities in LB films of C 60 -Be and comparing with that of CS 2 used as the reference. The value of χ xxxx (3) (2.1 x 10 -11 esu) was deduced at a 65 nm thick films. The χ (3) is attributed to a three-photon near resonance at the energy level of 29410 cm -1 . A new-type of two-chain amphiphilic molecule 1,10-bistearyl-4,6,13, 15-tetra-18-nitrogencrown-6 (NC) was used as insert material to construct mixed C 60 -Be/NC LB films. Our π-A, UV-visible absorption and SAXD measurements showed that the structural improvement in the mixed C 60 -Be/NC LB films was realized by insertion of the C 60 -Be molecules between the two hydrophobic chains of the NC molecules

Hypoxia-Mediated Epigenetic Regulation of Stemness in Brain Tumor Cells.

Science.gov (United States)

Prasad, Pankaj; Mittal, Shivani Arora; Chongtham, Jonita; Mohanty, Sujata; Srivastava, Tapasya

2017-06-01

Activation of pluripotency regulatory circuit is an important event in solid tumor progression and the hypoxic microenvironment is known to enhance the stemness feature of some cells. The distinct population of cancer stem cells (CSCs)/tumor initiating cells exist in a niche and augment invasion, metastasis, and drug resistance. Previously, studies have reported global hypomethylation and site-specific aberrant methylation in gliomas along with other epigenetic modifications as important contributors to genomic instability during glioma progression. Here, we have demonstrated the role of hypoxia-mediated epigenetic modifications in regulating expression of core pluripotency factors, OCT4 and NANOG, in glioma cells. We observe hypoxia-mediated induction of demethylases, ten-eleven-translocation (TET) 1 and 3, but not TET2 in our cell-line model. Immunoprecipitation studies reveal active demethylation and direct binding of TET1 and 3 at the Oct4 and Nanog regulatory regions. Tet1 and 3 silencing assays further confirmed induction of the pluripotency pathway involving Oct4, Nanog, and Stat3, by these paralogues, although with varying degrees. Knockdown of Tet1 and Tet3 inhibited the formation of neurospheres in hypoxic conditions. We observed independent roles of TET1 and TET3 in differentially regulating pluripotency and differentiation associated genes in hypoxia. Overall, this study demonstrates an active demethylation in hypoxia by TET1 and 3 as a mechanism of Oct4 and Nanog overexpression thus contributing to the formation of CSCs in gliomas. Stem Cells 2017;35:1468-1478. © 2017 AlphaMed Press.

Potencial terapêutico das células-tronco de medula óssea no tratamento da epilepsia Therapeutic potential of bone marrow stem cells in epilepsy treatment

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Maria Julia M. Carrion

2009-05-01

Full Text Available Epilepsia é uma patologia bastante prevalente no nosso meio. Há um número significativo de pacientes que não obtém resposta com a terapêutica medicamentosa, o que motivou a pesquisa de novas terapêuticas. O conceito de neurogênese no cérebro adulto, hoje já amplamente conhecida, principalmente a que ocorre na zona subventricular e na zona subgranular do giro denteado, motivou o desenvolvimeto de técnicas que aproveitassem esse mecanismo na tentativa de obtenção de efeitos antiepileptogênicos e reparadores. A maior parte dos estudos em vigência hoje, e que buscam tal finalidade, trabalha o uso de transplante de células progenitoras neurais ou de células fetais em modelos experimentais. No entanto, a terapêutica com células-tronco de medula óssea parece bastante interessante e promissora. Em doenças neurológicas nas quais os danos são frequentemente irreversíveis, as estratégias regenerativas podem representar um novo caminho. Em nosso laboratório, temos estudado o potencial terapêutico de células-tronco da medula óssea no controle de crises espontâneas recorrentes associadas ao modelo da pilocarpina com resultados excelentes. Também já está em andamento o primeiro estudo em humanos utilizando células-tronco de medula óssea para o tratatamento da epilepsia.Epilepsy is a prevalent pathology. A significative number of patients do not achieve an adequate response to pharmacological therapy. This observation has motivated the search for new strategies. The now well known concept of neurogenesis in the adult brain, in particular in the subventricular and dentate gyrus subgranular zones, has encouraged the development of strategies that might control this mechanism in order to obtain either antiepileptogenic or repair effects. Most studies focus on the transplantation of neural progenitor or fetal cells in experimental models, although bone marrow stem cell therapy is promising. In neurological diseases in which damage

Convenient method for the preparation of 1-acetoxy-1-methoxy-4-alkanones

International Nuclear Information System (INIS)

Kulinkovich, O.G.; Tishchenko, I.G.; Romashin, Yu.N.; Zaitsev, Yu.A.

1987-01-01

1-Acetoxy-1-methoxy-4-alkanones were obtained by the action of acetic acid on ethereal solutions of the corresponding alkyl trans-2-methoxycyclopropyl ketones. 1-Acetoxy-1-methoxy-4-decanone synthesized by this method was converted by the action of aqueous sodium hydroxide to 2-amyl-2-cyclopentenone in good yield. The PMR spectra of the compounds studied in CCl 4 were taken on a Tesla BS-467A spectrometer at 60 MHz with HMDS as the internal standard. The IR spectra were taken on a Specord 75-IR spectrophotometer

1,25 dihydroxyvitamin D3 (1,25) regulation of c-myc mRNA in HL-60 leukemia cells

International Nuclear Information System (INIS)

Simpson, R.U.; Bresnick, E.H.; Begley, D.A.

1986-01-01

Recently, 1,25 was shown to induce differentiation and decrease c-myc levels in HL-60 cells. The authors have confirmed these observations by RNA dot blot analysis. Cells treated with 50 nM 1,25 for 4, 24 and 48 hr showed c-myc mRNA levels of 26, 17 and 15% of control respectively. β-Actin mRNA levels were not altered. To ascertain whether 1, 25 regulated c-myc transcriptionally, an HL-60 nuclear RNA runoff assay was developed. Assay of total nuclei transcriptional activity revealed that 50% of RNA elongation was α-amanitin (0.8 μg/ml) sensitive and was linear with nuclei concentration (0.1-1 x 10 7 nuclei). 1,25 (50 nM) treated (45-96 hr) cells had decreased (approx.40%) total transcription rate relative to control. Decreased total RNA synthesis occurred concomitant with NBT reducing activity. 32 P-RNA runoff transcripts from HL-60 nuclei were hybridized to excess (5 μg DNA was excess) Pst I linearized c-myc and β-actin clones (in pBR322) immobilized on nitrocellulose filter. 32 P-RNA input from 2 x 10 6 to 2 x 10 7 cpm yielded linear hybridization signal. Analysis of blot dot intensity revealed no difference in transcription of c-myc in nuclei from 1,25 dosed or control cells. (myc/actin ratios: 1,25 (50 nM, 72 hr) =1.1 +/- 0.3 and control (72 hr) = 1.0, N=3 or 2 or 3 dots ea). These preliminary data suggest 1,25 does not affect c-myc transcription in HL-60 nuclei and may regulate c-myc mRNA post-transcriptionally

Relationship Between Structure and Antiproliferative Activity of Novel 5-amino-4-cyanopyrazole-1-formaldehydehydrazono Derivatives on HL-60RG Human Leukemia Cells.

Science.gov (United States)

Nagahara, Yukitoshi; Nagahara, Katsuhiko

2017-11-01

Pyrazole derivatives have been reported to have potent antimicrobial and anticancer activity. We recently synthesized and determined the effects of analogs, benzamidoxime derivatives, on mammalian cells and discovered that benzamidoximes had an antiproliferative effect. Here we synthesized and determined the anticancer effects of hydrazonopyrazole derivatives on a mammalian cancer cell line. We synthesized 12 hydrazonopyrazole derivatives with several constant alkyl chain length or branched chains at the side chain to investigate their anticancer cell activity, using the human myelogenous leukemia cell line HL-60RG. Among all hydrazonopyrazole derivatives we synthesized, the hydrazonopyrazole derivative with a branched chain at the side chain rather than a constant alkyl chain significantly inhibited cell viability. The strongest hydrazonopyrazole derivative, 5-amino-4-cyanopyrazole-1-formaldehydehydrazono-3'-pentanal, tended to damage cells dose-dependently. This cell growth attenuation was a result of apoptosis, activating caspase-3 and fragmented DNA. Hydrazonopyrazole derivatives induced apoptosis of HL-60RG leukemia cells. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells.

Science.gov (United States)

Naegelen, Isabelle; Plançon, Sébastien; Nicot, Nathalie; Kaoma, Tony; Muller, Arnaud; Vallar, Laurent; Tschirhart, Eric J; Bréchard, Sabrina

2015-03-01

Besides their roles in the killing of pathogens, neutrophils have the capacity to package a variety of cytokines into cytoplasmic granules for subsequent release upon inflammatory conditions. Because the rapid secretion of cytokines orchestrates the action of other immune cells at the infection site and thus, can contribute to the development and chronicity of inflammatory diseases, we aimed to determine the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation. From a constructed gene-expression network, we first selected relevant cytokines for functional validation by the CBA approach. We established a cytokine-secretion profile for human neutrophils and dHL-60 cells, underlining their similar ability to secrete a broad variety of cytokines within proinflammatory conditions mimicked by LPS stimulation. Secondly, after screening of SNARE genes by microarray experiments, we selected STX3 for further functional studies. With the use of a siRNA strategy, we show that STX3 is clearly required for the maximal release of IL-1α, IL-1β, IL-12b, and CCL4 without alteration of other cytokine secretion in dHL-60 cells. In addition, we demonstrate that STX3 is involved in MMP-9 exocytosis from gelatinase granules, where STX3 is partly localized. Our results suggest that the secretion of IL-1α, IL-1β, IL-12b, and CCL4 occurs during gelatinase degranulation, a process controlled by STX3. In summary, these findings provide first evidence that STX3 has an essential role in trafficking pathways of cytokines in neutrophil granulocytes. © Society for Leukocyte Biology.

41 CFR 60-1.41 - Solicitations or advertisements for employees.

Science.gov (United States)

2010-07-01

... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Solicitations or advertisements for employees. 60-1.41 Section 60-1.41 Public Contracts and Property Management Other Provisions Relating to Public Contracts OFFICE OF FEDERAL CONTRACT COMPLIANCE PROGRAMS, EQUAL EMPLOYMENT OPPORTUNITY, DEPARTMENT OF LABOR 1-OBLIGATIONS OF...

Estimulação da cicatrização óssea pelo plasma autógeno rico em plaquetas: estudo experimental em coelhos Bone healing stimulation by platelet-rich autogenous plasma: an experimental study in rabbits

Directory of Open Access Journals (Sweden)

Elisabete Mitiko Kobaiashi Wilson

2006-01-01

Full Text Available O plasma sangüíneo autógeno com alta concentração de plaquetas obtido por centrifugação (plasma rico em plaquetas, ou PRP tem sido utilizado na prática clínica para estimular a cicatrização óssea numa variedade de situações, sob alegação de que ele carrega uma elevada concentração de fatores de crescimento derivados da plaqueta e beta-transformadores, os quais sabidamente estimulam o crescimento e regeneração de diferentes tecidos. No presente estudo, o PRP foi utilizado para reparar uma falha óssea diafisária segmentar de meia espessura e 2 cm de comprimento produzida no radio de coelhos Nova Zelândia. O periósteo foi ressecado na circunferência do local da falha e a cavidade medular foi selada com cera óssea em todos os animais para bloquear a chegada de celular regeneradoras que não fossem do próprio osso, mas dos tecidos vizinhos. Três grupos de 15 animais cada foram planejados, de acordo com o procedimento realizado: 1 falha deixada vazia; 2 falha preenchida com o PRP; e 3 falha preenchida com um material inerte (Gelfoam®. Em cada grupo os animais foram distribuídos em três subgrupos de acordo com o período de observação pós-operatória, de 4, 8 e 12 semanas, respectivamente, depois do qual os animais foram mortos e o radio, ressecado para os estudos histológicos. Radiografias e cintilografias foram obtidas a intervalos de 4 semanas, começando na quarta semana pós-operatória. Cicatrização completa e remodelação foram observadas no grupo 2 já na 8ª semana, enquanto que nos grupos 1 e 3 esse processo era apenas parcial na 12ª. A captação do tecnécio estava aumentada em todos os grupos, assim permanecendo durante todo o período de observação nos grupos 1 e 3, mas diminuindo da 8ª para a 12ª semana no grupo 2, acompanhando o processo de remodelação, com diferenças significantes entre os grupos (pThe autogenous blood plasma with high platelet concentration obtained through centrifugation

Synthesis, characterization and anti cancer activity of some fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles

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Deepak Chowrasia

2017-05-01

Full Text Available A series of fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles (2a–2i was synthesized by condensation of various substituted 4-amino-5-phenyl-4H-1,2,4-triazole-3-thiols (1a–1i with penta fluoro benzoic acid in good yields (60–80%. The synthesized compounds were screened for anticancer activity against three cancerous cell lines MCF7 (human breast cancer, SaOS-2 (human osteosarcoma and K562 (human myeloid leukemia. The compounds showed moderate to good antiproliferative potency against the studied cell lines. Among these, compound 2b showed higher antiproliferative activity (IC50 22.1, 19 and 15 μM against MCF7, SaOS-2 and K562, respectively while 2a exhibited least antiproliferative activity (IC50 30.2, 39 and 29.4 μM against MCF7, SaOS-2 and K562 cells, respectively. Therefore, the present study demonstrates that fluorine substituted 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles would be a better prospective in the development of anticancer drugs.

East African Agricultural and Forestry Journal - Vol 60, No 4 (1995)

African Journals Online (AJOL)

Agro-Techniques for possible domestication of Gongronema latifolium · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. RAE Ugbaja, N Onuora. http://dx.doi.org/10.4314/eaafj.v60i4.46783 ...

Estudo da aderência de arames da armadura de tração em conectores de dutos flexíveis

OpenAIRE

Marcelo Badini Daflon

2010-01-01

Este projeto tem o objetivo de apresentar o estudo dos arames da armadura de tração que são empregados no transporte de óleo, gás e umbilicais hidráulicos na indústria petrolífera. Este estudo foi desenvolvido sob a necessidade de melhor ancoragem dos arames da armadura de tração de modo a garantir que, mesmo após a ruptura do arame dentro do conector não haverá o escorregamento do mesmo provocando a queda do duto flexível. Assim sendo, foi estabelecido o estudo de aderência da interface dos ...

The design study of the JT-60SU device. No. 4. The vacuum vessel and cryostat of JT-60SU

International Nuclear Information System (INIS)

Neyatani, Yuzuru; Ushigusa, Kenkichi; Tobita, Kenji

1997-03-01

The vacuum vessel and the cryostat for the JT-60 Super Upgrade (JT-60SU) have been designed. Two types of the complex materials for the vacuum vessel were chosen on the basis of the avoidance of tritium occlusion and the low irradiation, i.e. (1) SUS316 covered by tungsten plate (30mm thickness) as a γ-ray shielding, (2) Ti-6Al-4V alloy covered by SUS430 plate (1mm thickness) as a tritium protector. Selecting the double skin type of vacuum vessel with toroidally continued structure gave the basic design of the vacuum vessel satisfying the design criteria of the vessel strength for the electromagnetic force, heat load and the property of radiation shielding. The characteristics of the SUS316 covered by tungsten plate type is that as the tungsten can shield the γ-ray, the dose rate inside the vacuum vessel during the maintenance can reduce effectively. The advantage of the Ti-6Al-4V alloy covered by SUS430 plate type vacuum vessel is the quick reduction of the radioactive isotope because of no production of the isotopes with long half-life periods. Channel type and vertical type of the divertor were designed. The sector type of toroidally separated structure was selected for the remote handling. The material of the armor plate was not determined because no material endure the high heat load on the divertor. The cryostat composing the dome and the tank was designed. The electromagnetic force by the eddy current, generated at the plasma start up phase and at the quench of CS super-conducting coil, were small compared to the force produced by the stress limit. (author)

Involvement of the histamine H4 receptor in clozapine-induced hematopoietic toxicity: Vulnerability under granulocytic differentiation of HL-60 cells

International Nuclear Information System (INIS)

Goto, Aya; Mouri, Akihiro; Nagai, Tomoko; Yoshimi, Akira; Ukigai, Mako; Tsubai, Tomomi; Hida, Hirotake; Ozaki, Norio; Noda, Yukihiro

2016-01-01

Clozapine is an effective antipsychotic for treatment-resistant schizophrenia, but can cause fatal hematopoietic toxicity as agranulocytosis. To elucidate the mechanism of hematopoietic toxicity induced by clozapine, we developed an in vitro assay system using HL-60 cells, and investigated the effect on hematopoiesis. HL-60 cells were differentiated by all-trans retinoic acid (ATRA) into three states according to the following hematopoietic process: undifferentiated HL-60 cells, those undergoing granulocytic ATRA-differentiation, and ATRA-differentiated granulocytic cells. Hematopoietic toxicity was evaluated by analyzing cell survival, cell proliferation, granulocytic differentiation, apoptosis, and necrosis. In undifferentiated HL-60 cells and ATRA-differentiated granulocytic cells, both clozapine (50 and 100 μM) and doxorubicin (0.2 µM) decreased the cell survival rate, but olanzapine (1–100 µM) did not. Under granulocytic differentiation for 5 days, clozapine, even at a concentration of 25 μM, decreased survival without affecting granulocytic differentiation, increased caspase activity, and caused apoptosis rather than necrosis. Histamine H 4 receptor mRNA was expressed in HL-60 cells, whereas the expression decreased under granulocytic ATRA-differentiation little by little. Both thioperamide, a histamine H 4 receptor antagonist, and DEVD-FMK, a caspase-3 inhibitor, exerted protection against clozapine-induced survival rate reduction, but not of live cell counts. 4-Methylhistamine, a histamine H 4 receptor agonist, decreased the survival rate and live cell counts, as did clozapine. HL-60 cells under granulocytic differentiation are vulnerable under in vitro assay conditions to hematopoietic toxicity induced by clozapine. Histamine H 4 receptor is involved in the development of clozapine-induced hematopoietic toxicity through apoptosis, and may be a potential target for preventing its occurrence through granulocytic differentiation. - Highlights: • HL-60

Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics.

Directory of Open Access Journals (Sweden)

Federico Ferro

Full Text Available Although the role played by the core transcription factor network, which includes c-Myc, Klf4, Nanog, and Oct4, in the maintenance of embryonic stem cell (ES pluripotency and in the reprogramming of adult cells is well established, its persistence and function in adult stem cells are still debated. To verify its persistence and clarify the role played by these molecules in adult stem cell function, we investigated the expression pattern of embryonic and adult stem cell markers in undifferentiated and fully differentiated dental pulp stem cells (DPSC. A particular attention was devoted to the expression pattern and intracellular localization of the stemness-associated isoform A of Oct4 (Oct4A. Our data demonstrate that: Oct4, Nanog, Klf4 and c-Myc are expressed in adult stem cells and, with the exception of c-Myc, they are significantly down-regulated following differentiation. Cell differentiation was also associated with a significant reduction in the fraction of DPSC expressing the stem cell markers CD10, CD29 and CD117. Moreover, a nuclear to cytoplasm shuttling of Oct4A was identified in differentiated cells, which was associated with Oct4A phosphorylation. The present study would highlight the importance of the post-translational modifications in DPSC stemness maintenance, by which stem cells balance self-renewal versus differentiation. Understanding and controlling these mechanisms may be of great importance for stemness maintenance and stem cells clinical use, as well as for cancer research.

Pathways of topological rank analysis (PoTRA): a novel method to detect pathways involved in hepatocellular carcinoma.

Science.gov (United States)

Li, Chaoxing; Liu, Li; Dinu, Valentin

2018-01-01