Sample records for 有丝分裂 (mitosis)

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UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering

Cremer, Christoph; Cremer, Thomas; Hens, Luc; Baumann, Hella; Cornelis, Jan J.; Nakanishi, K.

UV micro-irradiation of a small part of the Chinese hamster nucleus and caffeine post-incubation often results in shattered chromosomes at the first post-irradiation mitosis. In some of these mitotic cells, chromosome shattering is restricted to a few chromosomes spatially related in a small area of...

DRIVER (Chinese)


Sensitivity of Synchronized Chinese Hamster Cells to Ultraviolet Light

Han, Antun; Sinclair, Warren K.

The age response for lethality of Chinese hamster cells to ultraviolet light shows that they are resistant in G1, sensitive as they move into and through the S phase and resistant again in G2 and mitosis. Survival curves determined at different times in the cycle reveal that mitotic cells are the mo...

DRIVER (Chinese)


Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase

Li, Shi-min; Yang, Hua; Meijer, Laurent; Zheng, Shu; Leclerc, Sophie; Zhang, Su-zhan; Yu, Lin-lin; Cheng, Jin-quan

Objective: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes ar...

DRIVER (Chinese)


Radiosensitivity of Mammalian Cells: IV. Effects of X-Irradiation on the DNA Synthetic Period in Synchronized Cells

Walters, R. A.; Tobey, R. A.

The effect of X-irradiation on the timing of DNA synthesis in the Chinese hamster ovary cells has been investigated. Mitotically synchronized cells irradiated in mitosis or early G1 exhibited a fixed, dose-independent (150-2000 rad) delay of 1.6 hr in entry into S, while the duration of S was unaffe...

DRIVER (Chinese)


Laser-UV-microirradiation of interphase nuclei and posttreatment with caffeine: a new approach to establish the arrangement of interphase chromosomes

Zorn, Christian; Cremer, Thomas; Cremer, Christoph; Zimmer, J.

Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three categories: I) intact metaphase figures, II) chromosome...

DRIVER (Chinese)


Inducers of DNA synthesis present during mitosis of mammalian cells lacking G1 and G2 phases.

Rao, P N; Sunkara, P S; Wilson, B A

The cell cycle analysis of Chinese hamster lung fibroblast V79-8 line by the premature chromosome condensation method has confirmed the absence of measurable G1 and G2 periods. Sendai virus-mediated fusion of mitotic V79-8 cells with G1 phase HeLa cells resulted in the induction of both DNA synthesi...

DRIVER (Chinese)


Disappearance of a structural chromatin protein A24 in mitosis: implications for molecular basis of chromatin condensation.

Matsui, S I; Sandberg, A A; Seon, B K

A chromatin protein, A24, a conjugate of histone H2A and evolutionally conserved ubiquitin, was virtually the only structural polypeptide that was present in interphase but missing in mitosis of a Chinese hamster cell line (DON). Because a 10% increase in the H2A/DNA ratio observed in interphase-mit...

DRIVER (Chinese)


Cdc2 Kinase-dependent Disassembly of Endoplasmic Reticulum (ER) Exit Sites Inhibits ER-to-Golgi Vesicular Transport during MitosisD⃞V⃞

Kondo, Hisao; Kano, Fumi; Yamauchi, Shinobu; Tanaka, Arowu R.; Murata, Masayuki

We observed the disassembly of endoplasmic reticulum (ER) exit sites (ERES) by confocal microscopy during mitosis in Chinese hamster ovary (CHO) cells by using Yip1A fused to green fluorescence protein (GFP) as a transmembrane marker of ERES. Photobleaching experiments revealed that Yip1A-GFP, which...

DRIVER (Chinese)