Sample records for 光 (light)
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66

X-Ray and Ultraviolet Sensitivity of Synchronized Chinese Hamster Cells at Various Stages of the Cell Cycle

Sinclair, W. K.; Morton, R. A.

Populations of Chinese hamster cells, synchronized by selecting for cells at or close to division, were exposed to 250 kvp x-rays and to ultraviolet light at different stages of the cell cycle and colony-forming ability examined thereafter. These cells were found to be most resistant to x-rays durin...

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67

Vegetation actualities and feasibility of restoration for the periphery of oasis in extreme arid region: taking Qira County of Xinjiang as an example Vegetation actualities and feasibility of restoration for the periphery of oasis in extreme arid region: taking Qira County of Xinjiang as an example

Ding;Jianli;Zhang;Ying(Ding)

Supported by National Natural Science Foundation of China (Grant No.40261006), West Light Foundation of The Chinese Academy of Science, Xinjiang Universities Scientific Research Plan (Grant No. XJEDU2004S03, XJEDU2005107), and Doctoral Start Fund of Xinjiang University.

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68

Ultraviolet Light-Induced Division Delay in Synchronized Chinese Hamster Cells

Han, Antun; Yu, C. K.; Sinclair, Warren K.

The age-dependent, ultraviolet light (UVL) (254 nm)-induced division delay of surviving and nonsurviving Chinese hamster cells was studied. The response was examined after UVL exposures adjusted to yield approximately the same survival levels at different stages of the cell cycle, 60% or 30% surviva...

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70

Ultrasonographic biomicroscopy, Scheimpflug photography, and novel provocative tests in contralateral eyes of Chinese patients initially seen with acute angle closure

Friedman, DS; Gazzard, G; Foster, P; Devereux, J; Broman, A; Quigley, H; Tielsch, J; Seah, S

OBJECTIVES: To compare ocular biometry of the contralateral eyes of individuals seen with acute angle closure (AAC) with eyes of population-based control subjects, and to assess novel provocative tests to study the mechanism of AAC. DESIGN: Prospective case-control study. PARTICIPANTS: Chinese perso...

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71

Thymidylate synthase-deficient Chinese hamster cells: a selection system for human chromosome 18 and experimental system for the study of thymidylate synthase regulation and fragile X expression.

Airhart, S D; Nussbaum, R L; Lesko, J G; Walmsley, R M; Ledbetter, D H

Chinese hamster lung (CHL) V79 cells already deficient in hypoxanthine phosphoribosyltransferase were exposed to uv light and selected for mutations causing deficiency of thymidylate synthase (TS) by their resistance to aminopterin in the presence of thymidine and limiting amounts of methyl tetrahyd...

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76

The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes.

Barker, P E; Czosnek, H; Shani, M; Nudel, U; Pravtcheva, D D; Ruddle, F H; Yaffe, D

The chromosomal distribution of murine genes expressed during differentiation of skeletal muscle cells was determined by Southern blot analysis of DNA from mouse-Chinese hamster hybrid cell lines containing incomplete subsets of mouse chromosomes. All detectable myosin heavy chain genes are located ...

DRIVER (Chinese)

78

Temperature-Sensitive Mutants of a Chinese Hamster Cell Line. I. Selection of Clones with Defective Macromolecular Biosynthesis

Roufa, Donald J.; Reed, Susan J.

Temperature-sensitive clones have been selected from a mutagenized culture of Chinese hamster lung cells by a procedure involving bromodeoxy-uridine (BrdU) incorporation and irradiation with black light. The selection procedure used in these studies was adapted from methods developed by others to yi...

DRIVER (Chinese)

88

Sensitivity of Synchronized Chinese Hamster Cells to Ultraviolet Light

Han, Antun; Sinclair, Warren K.

The age response for lethality of Chinese hamster cells to ultraviolet light shows that they are resistant in G1, sensitive as they move into and through the S phase and resistant again in G2 and mitosis. Survival curves determined at different times in the cycle reveal that mitotic cells are the mo...

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89

Risk factors for nuclear, cortical and posterior subcapsular cataracts in the Chinese population of Singapore: the Tanjong Pagar Survey

Foster, PJ; Wong, TY; Machin, D; Johnson, GJ; Seah, SKL

Aim: To describe risk factors for nuclear, cortical, and posterior subcapsular (PSC) cataracts in Chinese Singaporeans.Methods: A population based cross sectional study was carried out on ethnic Chinese men and women aged 40-81 years. A stratified, clustered, disproportionate ( more weights to older...

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91

Replication of adeno-associated virus in cells irradiated with UV light at 254 nm.

Winocour, E; Yakobson, B; Peak, M J; Hrynko, T A

Irradiation of simian virus 40 (ori mutant)-transformed Chinese hamster embryo cells (OD4 line) with UV light induced a cellular capacity which supported a full cycle of helper-independent adeno-associated virus replication. Monochromatic UV light at 254 nm was about 1,000-fold more effective than U...

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92

Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells.

Karentz, D; Cleaver, J E

Xeroderma pigmentosum (XP) is an autosomal recessive human disease, characterized by an extreme sensitivity to sunlight, caused by the inability of cells to repair UV light-induced damage to DNA. Cell fusion was used to transfer fragments of Chinese hamster ovary (CHO) chromosomes into XP cells. The...

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93

Recruitment of damaged DNA to the nuclear matrix in hamster cells following ultraviolet irradiation.

Koehler, D R; Hanawalt, P C

We examined the relationship between the nuclear matrix and DNA in the dihydrofolate reductase domain following irradiation of Chinese hamster cells with UV light. The fraction of matrix-bound DNA increased in transcribed and non-transcribed regions during a 3 h period after irradiation. However, no...

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94

Recovery after exposure to near-ultraviolet light of cells containing 5-bromodeoxyuridine.

Hagan, M P; Elkind, M M

The survival of synchronized V79 Chinese hamster cells irradiated with near-ultraviolet light after a 1-h labeling with 5-bromodeoxyuridine (BrdUrd) is highly dependent upon the cell's position in the cell cycle at the time of irradiation (Hagan, M., and M. M. Elkind. Biophys. J. 1979. 27:75-86). In...

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95

Professor John Bessant CV

Bessant, John

John Bessant is Professor of Innovation Management at the School of Management, Cranfield University. He graduated from Aston University with a degree in Chemical Engineering in 1975 and later obtained a PhD for work on innovation within the chemical industry. After a spell in industry he took up fu...

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96

Price strategy in the EU: Suggestions to Chinese exporters in the light of anti-dumping Price strategy in the EU: Suggestions to Chinese exporters in the light of anti-dumping

Nielsen, Jørgen Ulff-Møller

This paper investigates the EU anti-dumping policy towards Chinese companies. It is based on the EU anti-dumping cases since 1990. Based on this analysis, the paper presents practical advice to Chinese or foreign managers in companies in China with export to the EU. Firstly, the CELEX database may g...

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97

On intransitive nasal prefixation in Sino-Tibetan languages

Sagart, Laurent

The author discusses alternations of voiced and voiceless stop initials in intransitive/transitive pairs in Tibeto-Burman languages in the light of Chinese facts. He suggests that it is not necessary to suppose two distinct processes in proto-Tibeto-Burman to account for the facts, as Benedict's “al...

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98
99

Nascent DNA Synthesis in Ultraviolet Light-Irradiated Mouse, Human, and Chinese Hamster Cells

Rauth, A. M.; Hunter, G.; Tammemagi, M.

The technique of alkaline sucrose gradient centrifugation was used to study newly synthesized DNA in control and ultraviolet light-irradiated mouse L, human HeLa, and Chinese hamster ovary cells. Nascent DNA molecular weight distributions did not appear to differ among the three cell lines for unirr...

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100

Mutant hamster cells exhibiting a pleiotropic effect on carbohydrate metabolism.

Sun, N C; Chu, E H; Chang, C C

Sixty-seven galactose negative (Gal minus) clones were established from survivors after treatment of Chinese hamster lung (V79) cells with 5-bromodeoxyuridine and black light. The mutational origin of these clonal isolates was inferred on the basis of the previously demonstrated mutagenic action of ...

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104

Integrated economy-energy-environment policy analysis: a case study for the People's Republic of China.

Zhang, Z.X.

This study is the first systematic and comprehensive attempt to deal with the economic implications of carbon abatement for the Chinese economy in the light of the economics of climate change, of which this dissertation is the results. It consists of nine chapters. After a brief introduction, Chapte...

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105

Induction of chromosome shattering by ultraviolet light and caffeine: The influence of different distributions of photolesions

Cremer, Christoph; Cremer, Thomas

Cells of synchonized and of asynchronously growing cultures of a V79 Chinese hamster line were microirradiated with a low poweer laser-UV-microbeam of wavelength 257 nm. Ultraviolet light was either focused onto a small part of the nucleus (mode I) or distributed over the whole nucleus (mode II). Fo...

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106

Induction of chromosome shattering by ultraviolet light and caffeine

Cremer, Christoph; Cremer, Thomas; Zorn, Christian; Zimmer, J.

Synchronized and asynchronously growing cells of a V79 sub-line of the Chinese hamster were either partial-cell irradiation (λ, 254 nm) or laser-UV-microirradiated (λ, 257 nm). Post-incubation with caffeine (1–2 mM) often resulted in chromosome shattering, which was a rare event in the absence of th...

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107

Induction of chromosome damage by ultraviolet light and caffeine: Correlation of cytogenetic evaluation and flow karyotype

Cremer, Christoph; Cremer, Thomas; Gray, J. W.

Asynchrononously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated ( = 254 nm) with UV fluences up to 7.5 J/m2. After irradiation, cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected by mechanical shaking. Their chromosomes were ...

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108

Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light

Chu, E. H. Y.; Chang, C. C.; Sun, N. C.

Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridin...

DRIVER (Chinese)

109

Imaging of luciferase secretion from transformed Chinese hamster ovary cells.

Tsuji, F I; Inouye, S; Toya, Y; Ohmiya, Y

The blue luminescence characteristic of the marine ostracod crustacean Vargula hilgendorfii is from a simple, but highly specific, enzyme-substrate reaction. Light is emitted by the oxidation of Vargula luciferin (substrate) by molecular oxygen, a reaction catalyzed by luciferase. Stable transforman...

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114

Genetics of Somatic Mammalian Cells: Biochemical Genetics of Chinese Hamster Cell Mutants with Deviant Purine Metabolism

Patterson, David; Puck, Theodore T.; Kao, Fa-Ten

Studies are presented on the biochemical genetics of 30 adenine-requiring mutants of the Chinese hamster ovary cell which were induced by mutagenesis and selected by the BrdU-visible light technique. Representative experiments conducted with these mutants include: hybridization with each other; hybr...

DRIVER (Chinese)

115

Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.

Mooney, C L; Thompson, L H; Brookman, K; Busch, D B; Glaser, D A

Mutant lines of Chinese hamster ovary cells that show hypersensitivity to killing and mutagenesis by UV light were analyzed by genetic complementation analysis to determine whether defects in different gene loci might underlie a common cellular phenotype,. To facilitate rapid screening of mutant clo...

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116

Gender and Identity in Mandarin Chinese Blogosphere

Siqun, Xu

The research on gender and identity in Mandarin Chinese CMC sphere has been less explored from a sociolinguistic point of view, which motivates me to do some relevant studies to shed light on the issues of gender difference and online identity in Mandarin Chinese blogosphere. In this project, both p...

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117

Enhancement of postreplication repair in ultraviolet-light-irradiated Chinese hamster cells by irradiation in G2 or S-phase.

D'Ambrosio, S M; Setlow, R B; Aebersold, P M

Postreplication repair in synchronous Chinese hamster cells was determined after split doses of ultraviolet (UV) radiation. Repair was enhanced by irradiation of cells in G2 or S-phase with a small dose of UV radiation at least 1.5 h before a three-fold larger dose of UV. There was significantly gre...

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119

Effects of ultraviolet irradiation on the rate and sequence of DNA replication in synchronized Chinese hamster cells.

Humphrey, R M; Meyn, R E; Thomas, L F; Hewitt, R R

The effects of ultraviolet light (UV) irradiation on the rate of DNA replication in synchronized Chinese hamster ovary (CHO) cells were investigated. A technique for measuring semiconservative DNA replication was employed that involved growing the cells in medium containing 5-bromodeoxyuridine and s...

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120

Effect of von Willebrand factor coexpression on the synthesis and secretion of factor VIII in Chinese hamster ovary cells.

Wise, R J; Kaufman, R J; Davies, M V; Wasley, L C; Israel, D I; Dorner, A J

In plasma, antihemophilic factor (factor VIII) exists as a 200-kilodalton heavy-chain polypeptide in a metal ion association with an 80-kilodalton light-chain polypeptide. This complex is bound by hydrophobic and hydrophilic interactions to a large multimeric glycoprotein, von Willebrand factor (vWF...

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121

Effect of Clostridium difficile enterotoxin A on ultrastructure of Chinese hamster ovary cells.

Kushnaryov, V M; Sedmak, J J

Electron microscopical immunocytochemistry and light microscopy were used to study the effect of Clostridium difficile enterotoxin A (EA) on cultured Chinese hamster ovary (CHO) cells. At 4 degrees C, immunocytochemically detected EA was randomly distributed along the plasma membrane; when cells wer...

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122

Deoxyribonucleic Acid Synthesis in Ultraviolet-Light-Irradiated Chinese Hamster Cells

Meyn, Raymond E.; Humphrey, Ronald M.

Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability between fractionated doses of ultraviolet light (UV), and Chinese hamster B-14FAF28 which cannot recover, were tested for the ability to bypass UV-induced photoproducts in DNA during postirradiation DNA sy...

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124

Damage and Repair of DNA in 5-Bromodeoxyuridine-Labeled Chinese Hamster Cells Exposed to Fluorescent Light

Ben-Hur, E.; Elkind, M. M.

Illumination of Chinese hamster cells with fluorescent light after 5-bromodeoxyuridine incorporation leads to extensive single-strand breakage in the DNA of the exposed cells. The rate of production of single-strand breaks is dependent on the extent to which thymine is replaced by 5-bromouracil. At ...

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125

DNA-protein complexes induced by chromate and other carcinogens.

Costa, M

DNA-protein complexes induced in intact Chinese hamster ovary cells by chromate have been isolated, analyzed, and compared with those induced by cis-platinum, ultraviolet light, and formaldehyde. Actin has been identified as one of the major proteins complexed to DNA by chromate based upon its molec...

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126

DNA strand specificity for UV-induced mutations in mammalian cells.

Zdzienicka, M Z; Vrieling, H; Groen, N A; Van Rooijen, M L; Simons, J W; Lohman, P H; van Zeeland, A A

The influence of DNA repair on the molecular nature of mutations induced by UV light (254 nm) was investigated in UV-induced hprt mutants from UV-sensitive Chinese hamster cells (V-H1) and the parental line (V79). The nature of point mutations in hprt exon sequences was determined for 19 hprt mutant...

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127

DNA repair in the metallothionein gene increases with transcriptional activation.

Okumoto, D S; Bohr, V A

We have studied DNA repair in the Chinese Hamster Ovary (CHO) metallothionein (MT) gene after UV-light induced damage. The repair was examined comparatively with or without transcriptional activation of the gene by incubation in the presence of the heavy metal ZnCl2. Whereas the repair efficiency wa...

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128

Cooperative effects of photodynamic treatment of cells in microcolonies

Steen, Harald B.; Dahle, Jostein; Moan, Johan; Kaalhus, Olav

Microcolonies of 2-8 Madison–Darby canine kidney cells (MDCK II) and Chinese hamster lung fibroblasts (V79) cells were incubated with the photosensitizer Photofrin and exposed to light, and the resulting number of dead cells per colony was determined. The distribution of this number was found to be ...

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129

Chinesen in Bukarest : Diskurse und Geschichten Chinese in Bucharest : discourses and stories

Wundrak, Rixta

In dieser ethnographisch-soziologischen Fallstudie wird die Geschichte der chinesischen Immigration in Bukarest nach 1990 in ihrer Besonderheit und ihren allgemeinen Strukturen nachvollzogen. Dies geschieht durch eine Doppelperspektive auf den Fall: Die Rekonstruktion der diskursiven (Re-)Präsentati...

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131

Changes in repair competency after 5-bromodeoxyuridine pulse labeling and near-ultraviolet light.

Hagan, M P; Elkind, M M

Synchronized V79 Chinese hamster cells, pulse-labeled with 5-bromodeoxyuridine (BrdUrd), show marked changes in the sensitivity to near-ultraviolet light during the cell cycle. Cells are least sensitive during the remainder of the S-phase after the BrdUrd pulse. They become maximally sensitive in th...

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132

Cell Proliferation and DNA Breaks Are Involved in Ultraviolet Light-induced Apoptosis in Nucleotide Excision Repair-deficient Chinese Hamster Cells

Dunkern, Torsten R.; Kaina, Bernd

UV light targets both membrane receptors and nuclear DNA, thus evoking signals triggering apoptosis. Although receptor-mediated apoptosis has been extensively investigated, the role of DNA damage in apoptosis is less clear. To analyze the importance of DNA damage induced by UV-C light in apoptosis, ...

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134

Aids in China: Discourses on Sexuality and Sexual Practices

Micollier, Evelyne

The issues that have taken shape around the need to prevent the spread of HIV/Aids, and to manage the risks of an epidemic, throw light on the pressing contradictions within Chinese society. They offer a very relevant approach for analysis of current changes in the field of sexuality and to understa...

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136

A 22-kd protein (sorcin/V19) encoded by an amplified gene in multidrug-resistant cells, is homologous to the calcium-binding light chain of calpain.

Hes, E; Van der Bliek, A M; Biedler, J L; Meyers, M B; Borst, P

We have previously shown that at least five linked genes are co-amplified and overexpressed in the multi-drug resistant (MDR) Chinese hamster ovary cell line CHRC5. We show here that one of these genes (class 4) codes for a small phosphorylated, cytosolic protein, sorcin/V19, known to be overproduce...

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137

Allen, Nicholas J.

The Buddha's life-story has been told in many forms, but the earliest sources (in Pali and Chinese) were made available in French by Andre Bareau in 1963. Using Bareau, I study the story in the light of Indo-European cultural comparativism. The Founder's experiences between leaving home and making h...

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138

5-bromodeoxyuridine-DNA strand symmetry and the repair of photolytic breaks in Chinese hamster cell chromosomes.

Roufa, D J

Experiments described in this report quantiate the black light sensitivities of Chinese hamster lung (CHL) cells containing BrdUrd-DNA of defined composition. Cesium chloride equilibrium gradient centrifugation provides estimates both of the percent thymidine replacement by BrdUrd and of the symmetr...

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