Sample records for 水平面 (levels)
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13

山东、四川部分区县环境和食物中氟化物水平调查 Investigation on Fluoride Levels tn Environment and Food

LI Shu - rain(李淑敏); YING Bo(应波); Jl Rortg - di(吉荣娣)

目的了解调查地区环境和食物中氟化物的水平。方法采用燃烧水解一氟离子选择电极法测定土壤中氟化物的含量;离子选择电极法直接测定饮水中氟化物的含量,扩散一氟离子选择电极法测定粮食和蔬菜样品中氟化物的含量。结果山东和四川省调查地区饮用水氟化物的含量为0.14~39mg/L,符合我国生活饮用水卫生标准(GB5749—1985);调查地区土壤氟化物含量为175.4~625.1mg/kg;调查地区6种粮食氟化物的含量,除四川邛崃县面粉外,其他均符合我国粮食含氟标准;调查地区蔬菜氟化物的含量除山东淄川的韭菜、油菜氟超出限值(1mg/kg)4倍和0.9倍。四川邛崃干辣椒氟超出限值(1mg/kg)4倍外,其余各类...

DRIVER (Chinese)

29

四川省适宜硒与低硒地区环境硒水平调查结果分析 Environmental selenium level survey in areas with enough or low selenium in Sichuan Province

ZHU Lan(朱岚); LI De-yun(李德云); LI Ping(李平); ZHOU Ding-you(周定友); ZHAO Yong-mei.(赵咏梅)

目的了解四川省不同地理环境硒水平状况。方法根据四川省部分地区儿童发硒的分布,选择儿童发硒水平低于0.2mg/kg的剑阁县作为缺硒水平地区和高于0.2mg/kg的双流县作为适宜硒水平地区,调查粮食、土壤、饮水和指甲中含硒量。结果剑阁县大米、小麦,土壤、指甲含硒量分别为0.0160、0.0208、0.0005、0.2276mg/kg;双流县大米、小麦、土壤、指甲含硒量分别为0.0460、0.0510、0.0054、0.5241mg/kg;双流县粮食、土壤和指甲含硒量明显高于剑阁县,两个地区比较差异有统计学意义(t大米=9.37,P〈0.01;t小麦=6.99,P〈0.01;t土壤=4.53,P〈0...

DRIVER (Chinese)

32

Water discharge and sediment flux changes over the past decades in the Lower Mekong River: possible impacts of the Chinese dams

Lu, X. X.; Siew, R. Y.

The Lower Mekong River has witnessed extremely low water levels over the past few years. There is speculation that the changes are a consequence of the construction and operation of the Chinese cascade dams in the upper part of the Mekong main stream, the Lancang River. Dam construction on upper str...

DRIVER (Chinese)

33

Water discharge and sediment flux changes in the Lower Mekong River

Lu, X. X.; Siew, R. Y.

The Lower Mekong River has witnessed extremely low water levels over the past few years. There is speculation that the changes are a consequence of the construction and operation of the Chinese cascade dams in the upper part of the Mekong main stream, the Lancang River. Dam construction on upper str...

DRIVER (Chinese)

34

Verification of the formulation and efficacy of Danggui Buxue Tang (a decoction of Radix Astragali and Radix Angelicae Sinensis): an exemplifying systematic approach to revealing the complexity of Chinese herbal medicine formulae

Dong, Tina Tingxia; Cheung, Anna Wing Han; Duan, Jinao; Gao, Qiutao; Li, Jun; Li, Winnie Zhuoming; Zhao, Kuijun; Tsim, Karl Wah Keung

This article exemplifies a systematic approach to revealing the complexity of Chinese herbal medicine formulae through three levels of scientific research: standardization of herbs, verification of ancient formulae and mechanism studies. We use Danggui Buxue Tang (DBT) as an example for this approac...

DRIVER (Chinese)

36

Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.

Purchio, A F; Brunner, A M; Gentry, L E; Lim, G J; Webb, N R; Ranchalis, J E; Marquardt, H; Twardzik, D R; Lioubin, M N

Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcripti...

DRIVER (Chinese)

37

Transfer and amplification of a mutant beta-tubulin gene results in colcemid dependence: use of the transformant to demonstrate regulation of beta-tubulin subunit levels by protein degradation.

Gottesman, M M; Whitfield, C; Ascherman, D; Abraham, I

Total genomic DNA from a temperature-sensitive, colcemid-resistant Chinese hamster ovary (CHO) cell mutant expressing an electrophoretic variant beta-tubulin was used to transform wild-type CHO cells to colcemid-resistant cells at 37 degrees C. Southern blot analysis of the transformant demonstrated...

DRIVER (Chinese)

39

Tissue-specific cadmium accumulation and metallothionein-like protein levels during acclimation process in the Chinese crab Eriocheir sinensis.

Silvestre, F; Duchêne, C; Trausch, G; Devos, P.

Aquatic organisms chronically exposed to cadmium can increase their resistance to a subsequent elevated exposure. In order to investigate mechanisms involved in acclimation process in the Chinese crab Eriocheir sinensis, we compared Cd level as well as metallothionein-like protein (MTLP) content in ...

DRIVER (Chinese)

42

The effect of dietary n-3 HUFA levels and DHA/EPA ratios on growth, survival and osmotic stress tolerance of Chinese mitten crab Eriocheir sinensis larvae

Sui, L.; Wille, M.; Cheng, Y.; Sorgeloos, P.

The effect of varying levels of dietary n-3 highly unsaturated fatty acid (HUFA) and docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) ratios on growth, survival and osmotic stress tolerance of Eriocheir sinensis zoea larvae was studied in two separate experiments. In experiment I, larvae ...

DRIVER (Chinese)

44

The apolipoprotein E polymorphism: a comparison of allele frequencies and effects in nine populations.

Sandholzer, C; Hallman, D M; Saha, N; Boerwinkle, E; Menzel, H J; Csázár, A; Utermann, G

Application of uniform methods for measuring the apolipoprotein (apo) E polymorphism and plasma cholesterol levels in nine populations (Tyrolean, Sudanese, Indian, Chinese, Japanese, Hungarian, Icelandic, Finnish, and Malay) revealed significant heterogeneity among them in apo E type frequencies and...

DRIVER (Chinese)

49

TNFalpha-Polymorphismus bei Patienten mit Sepsis und Schilddrüsenkarzinomen

Rossbach, Christiane

Das Zytokin Tumornekrosefaktor a (TNFa) hat einen entscheidenden Anteil an der Entwicklung schwerer Komplikationen, wie septischer Schock und Multiorganversagen nach Entwicklung einer Sepsis. Eine Assoziation des TNF2-Allels mit einem erhöhten TNF a Spiegel und einer höheren Mortalitätsrate wurde i...

DRIVER (Chinese)

55

Serum Uric Acid Levels of Healthy Caucasian, Chinese and Haida Indian Males in British Columbia

Ford, Denys K.; deMos, Agatha M.

Because previous studies have shown that serum uric acid levels are higher in members of the Filipino and Maori races than in Caucasians, and because gout seemed unexpectedly common in the Chinese population of Vancouver, a study of serum uric acid levels in Caucasian, Chinese and Haida Indian males...

DRIVER (Chinese)

56

Segregation and rearrangement of coamplified genes in different lineages of mutant cells that overproduce adenylate deaminase.

de Saint-Vincent, B R; Debatisse, M; Petit-Koskas, E; Hyrien, O; Buttin, G

Four genes encoding proteins designated as W, X, Y1, and Y2 were found previously to be amplified at different levels in a Chinese hamster fibroblast mutant line selected for overproduction of adenylate deaminase. To gain information on the molecular mechanisms responsible, we studied the levels of ...

DRIVER (Chinese)

61

S phase-specific synthesis of dihydrofolate reductase in Chinese hamster ovary cells.

Mariani, B D; Schimke, R T; Slate, D L

We investigated the cell cycle modulation of dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase, 7,8-dihydroxyfolate:NADP+ oxidoreductase, EC 1.5.1.3) levels in methotrexate-resistant Chinese hamster ovary cells synchronized by mitotic selection. DNA content and DHFR concentration were an...

DRIVER (Chinese)

63

Relationship of postprandial serum gastrin response to sex, body weight, blood group status, familial dyspepsia, duration, and age of onset of ulcer symptoms in duodenal ulcer.

Lam, S K; Ong, G B

Integrated postprandial serum gastrin levels were studied in a prospective series of 144 Chinese patients with duodenal ulcer in relation to sex, total body weight, age of onset and duration of ulcer symptoms, blood group status, and positivity for familial dyspepsia. Postprandial gastrin was unrela...

DRIVER (Chinese)

65

Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells.

Dorner, A J; Kaufman, R J; Krane, M G

GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site seq...

DRIVER (Chinese)

66

Protein-DNA interactions at the major and minor promoters of the divergently transcribed dhfr and rep3 genes during the Chinese hamster ovary cell cycle.

Heintz, N H; Wells, J; Illenye, S; Held, P

In mammals, two TATA-less bidirectional promoters regulate expression of the divergently transcribed dihydrofolate reductase (dhfr) and rep3 genes. In CHOC 400 cells, dhfr mRNA levels increase about fourfold during the G1-to-S phase transition of the cell cycle, whereas the levels of rep3 transcript...

DRIVER (Chinese)

67

Protein dissociation from GRP78 and secretion are blocked by depletion of cellular ATP levels.

Dorner, A J; Kaufman, R J; Wasley, L C

Secretory proteins expressed in Chinese hamster ovary (CHO) cells interact to various degrees with glucose-regulated protein 78 (GRP78), a resident protein of the endoplasmic reticulum. von Willebrand factor (vWF) and wild-type tissue plasminogen activator (tPA) are efficiently secreted and exhibit ...

DRIVER (Chinese)

72

Preliminary research on Megalake Jilantai-Hetao in the arid areas of China during the Late Quaternary

Chen, Fahu; Fan, Yuxin; Chun, Xi; MADSEN, D.B.; OVIATT, C.G.; Zhao, Hui; Yang, Liping; Sun, Yang

We identified geomorphological, sedimentary and biological evidence of high lake levels around the current Jilantai Salt Lake through field investigations and through analyses of regional remote sensing images. There are four groups of shorelines at elevations of around 1060, 1050, 1044 and 1035 m a...

DRIVER (Chinese)

85

Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese

Hu, Suwei; Zhong, Yanfang; Hao, Yuantao; Luo, Minqi; Zhou, Yan; Liao, Weijiao; Wei, Haiyun; Gao, Yueting; Shan, Jinglan

Background: High-density lipoprotein (HDL) is a major plasma lipoprotein directly associated with cholesterol metabolism. The ATP binding cassette transporter 1 gene (ABCA1) is one of the major genes modulating plasma levels of HDL-cholesterol (HDL-C). Rare alleles of ABCA1 associated with extreme H...

DRIVER (Chinese)

86

Nonsense mutations in the dihydrofolate reductase gene affect RNA processing.

Chasin, L A; Urlaub, G; Ciudad, C J; Mitchell, P J

Steady-state dihydrofolate reductase (dhfr) mRNA levels were decreased as a result of nonsense mutations in the dhfr gene. Thirteen DHFR-deficient mutants were isolated after treatment of Chinese hamster ovary cells with UV irradiation. The positions of most point mutations were localized by RNA het...

DRIVER (Chinese)

87

Nick-translation of metaphase chromosomes: in vitro labeling of nuclease-hypersensitive regions in chromosomes.

Kuo, M T; Plunkett, W

Chinese hamster metaphase chromosomes were labeled by nick-translation, which involved pretreatment of metaphase chromosomes with low levels of DNase I followed by incubation with DNA polymerase I and radioactively labeled nucleotides. The labeled DNA was located on nuclease-hypersensitive regions o...

DRIVER (Chinese)

88

New amber mutation in a beta-thalassemic gene with nonmeasurable levels of mutant messenger RNA in vivo.

Kazazian, H H; Atweh, G F; Zhu, X X; Brickner, H E; Forget, B G

We have identified a beta-thalassemia gene that carries a novel nonsense mutation in a Chinese patient. This mutation, a G to T substitution at the first position of codon 43, changes the glutamic acid coding triplet (GAG) to a terminator codon (TAG). Based on oligonucleotide hybridization studies o...

DRIVER (Chinese)

90

Molecular events in the processing of recombinant type 1 pre-pro-transforming growth factor beta to the mature polypeptide.

Marquardt, H; Gentry, L E; Purchio, A F; Lioubin, M N

Recently, the simian type 1 transforming growth factor beta (TGF-beta 1) cDNA was expressed at high levels in Chinese hamster ovary (CHO) cells by dihydrofolate reductase-induced gene amplification (L.E. Gentry, N.R. Webb, G.J. Lim, A.M. Brunner, J.E. Ranchalis, D.R. Twardzik, M.N. Lioubin, H. Marqu...

DRIVER (Chinese)

100

Lower intracellular hydrogen peroxide levels in cells overexpressing CuZn-superoxide dismutase

Teixeira, Henrique D.; Meneghini, Rogerio; Schumacher, Robert I.

Transfection of V79 Chinese hamster cells produced clones in which CuZn-superoxide dismutase (CuZn-SOD) activities were 2.2- to 3.5-fold higher than in the parental cells. An overall reduction of antioxidant enzyme activities and both total and oxidized glutathione levels had been found in these clo...

DRIVER (Chinese)

101

Low levels of cardiovascular risk factors and coronary heart disease in a UK Chinese population.

Harland, J O; Unwin, N; Bhopal, Raj; White, M; Watson, B; Laker, M; Alberti, K G

OBJECTIVE: To compare the prevalence of cardiovascular risk factors and coronary heart disease in Chinese and Europid adults. DESIGN: Population based, cross sectional survey. SETTING: Newcastle upon Tyne, UK, 1991-93. SUBJECTS: Altogether 380 Chinese and 625 Europid adults, aged 25-64 years. MAIN O...

DRIVER (Chinese)

102

Insulin-stimulated disassociation of the SOS-Grb2 complex.

Waters, S B; Pessin, J E; Yamauchi, K

Insulin stimulation of differentiated 3T3-L1 adipocytes or Chinese hamster ovary cells expressing high levels of the insulin receptor resulted in a time-dependent decrease in the electrophoretic mobility of SOS on sodium dodecyl sulfate-polyacrylamide gels. The reduction in SOS mobility was complete...

DRIVER (Chinese)

103

Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.

Gantt, J S; Arfin, S M; Bennett, C A

The growth of Chinese hamster ovary cells in medium containing reduced concentrations of threonine is inhibited by borrelidin, a macrolide antibiotic. Borrelidin-resistant clones have been isolated after ethyl methanesulfonate mutagenesis. One clone, 1C-1, has a 3-fold increased level of threonyl-tR...

DRIVER (Chinese)

104

In vitro translation of hypoxanthine/guanine phosphoribosyltransferase mRNA: characterization of a mouse neuroblastoma cell line that has elevated levels of hypoxanthine/guanine phosphoribosyltransferase protein.

Hejtmancik, J F; Melton, D W; Ledbetter, D H; Konecki, D S; Caskey, C T

Antibody specific for the native form of Chinese hamster hypoxanthine/guanine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) was used to detect the synthesis of HPRT protein in a rabbit reticulocyte lysate translation system primed with mRNA from Chinese ha...

DRIVER (Chinese)

105

Impact of cadmium exposure on male sex hormones: a population-based study in China

Zeng, XB; Jin, TY; Buchet, Jean-Pierre; Jiang, XZ; Kong, QH; Ye, TT; Bernard, Alfred; Nordberg, GF

The objective of this study was to investigate the possible effects of environmental cadmium (Cd) exposure on the levels of serum sex hormones in a Chinese population group. A total of 263 male volunteers were included. Blood samples were collected for the determination of serum testosterone (T), me...

DRIVER (Chinese)

114

Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; Van Wormhoudt, Alain

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutam...

DRIVER (Chinese)

115

Genotoxicity of 1,4-benzoquinone and 1,4-naphthoquinone in relation to effects on glutathione and NAD(P)H levels in V79 cells.

Ludewig, G; Glatt, H; Dogra, S

1,4-Benzoquinone is cytotoxic in V79 Chinese hamster cells and induces gene mutations and micronuclei. The cell-damaging effects of quinones are usually attributed to thiol depletion, oxidation of NAD(P)H, and redox-cycling involving the formation of semiquinone radicals and reactive oxygen species....

DRIVER (Chinese)

116

Gama-aminobutyric acid accumulation in Elsholtzia splendens in response to copper toxicity*

Yang, Xiao-e; Tian, Sheng-ke; Peng, Hong-yun

A solution with different Cu supply levels was cultured to investigate gama-aminobutyric acid (GABA) accumulation in Elsholtzia splendens, a native Chinese Cu-tolerant and accumulating plant species. Increasing Cu from 0.25 to 500 μmol/L significantly enhanced levels of GABA and histidine (His), but...

DRIVER (Chinese)

117

Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions

Cederfur, Cecilia

Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since...

DRIVER (Chinese)

118

GUANIDINE TO ADENINE (G/A) SUBSTITUTION IN THE PROMOTER REGION OF THE APOLIPOPROTEIN AI GENE IS ASSOCIATED WITH ELEVATED SERUM APOLIPOPROTEIN AI LEVELS IN CHINESE NONSMOKERS

SAHA, N; TAY, JSH; LOW, PS; HUMPHRIES, SE

The influence of the guanidine to adenine (G/A) substitution in the promoter region of the apolipoprotein (apo) AI gene (at -75 bp) on serum lipids and apolipoproteins was studied in 287 healthy Chinese of both sexes in Singapore. Women had significantly higher levels of high-density lipoprotein cho...

DRIVER (Chinese)

120

Evidence of high levels of methylglyoxal in cultured Chinese hamster ovary cells

Chaplen, Frank W. R.; Cameron, Douglas C.; Fahl, William E.

Methylglyoxal is an α-ketoaldehyde and dicarbonyl formed in cells as a side product of normal metabolism. Endogenously produced dicarbonyls, such as methylglyoxal, are involved in numerous pathogenic processes in vivo, including carcinogenesis and advanced glycation end-product formation; advanced g...

DRIVER (Chinese)

121

Ethylene Production and 1-Aminocyclopropane-l-Carboxylate (ACC) Synthase Gene Expression in Tomato ( L ycopsicon esculentum Mill.) Leaves Under Enhanced UV-B Radiation

An, Lizhe; Xu, Xiaofeng; Tang, Hongguan; Zhang, Manxiao; Hou, Zongdong; Liu, Yanhong; Zhao, Zhiguang; Feng, Huyuan

[英文摘要]: Tomato (Lycopslcon esculentum Mill.) plants grown in a greenhouse were irradiated with two different levels of UV-B, namely 8.82 (T1) and 12.6 kJ/m^2 per day (T2). Ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) content, 1-(malonylamino) cyclopvopane-1-carboxylic acid (MA...

DRIVER (Chinese)

122

Escherichia coli heat-labile enterotoxin: comparison of antitoxin assays and serum antitoxin levels.

Wachsmuth, I K; Ryder, R W; Wells, J G

The mouse adrenal tumor cells (Y-1 strain) and the Chinese hamster ovary cells, two routinely used tissue culture assays for Escherichia coli heat-labile enterotoxin (LT), were used to detect serum antitoxin responses in culture-positive patients from several well-defined sources. There was no corre...

DRIVER (Chinese)

123

Epidermal growth factor receptor is increased in multidrug-resistant Chinese hamster and mouse tumor cells.

Biedler, J L; Meyers, M B; Spengler, B A; Merluzzi, V J

Multidrug-resistant sublines of Chinese hamster lung and mouse tumor cells selected for high levels of resistance to vincristine or actinomycin D have increased numbers of epidermal growth factor (EGF) receptors compared to control cells. Evidence for this increase was found in six of six resistant ...

DRIVER (Chinese)

124

Enhancement or inhibition of insulin signaling by insulin receptor substrate 1 is cell context dependent.

Yamauchi, K; Pessin, J E

Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin...

DRIVER (Chinese)

125

Enhancement in amount of P1 (hsp60) in mutants of Chinese hamster ovary (CHO-K1) cells exhibiting increases in the A system of amino acid transport.

Jones, M; Englesberg, E; Gupta, R S

Mutants of CHO-K1 cells with varied levels of A system activity, probably the result of increases in absolute amount of the A system transporter, have corresponding increases in levels of peptides banding at 62-66 and 29 kDa. Mutant alar4-H3.9, showing the highest increase of A system activity and o...

DRIVER (Chinese)

126

Elongation of the Polyadenylate Segment of Messenger RNA in the Cytoplasm of Mammalian Cells

Diez, J.; Brawerman, G.

Chinese hamster and mouse sarcoma 180 ascites cells, treated with high levels of actinomycin D, still carry out limited poly(A) synthesis. The residual activity, which consists of poly(A) chain extension in the cytoplasm as well as in the nucleus, is essentially insensitive to cordycepin. Nuclear po...

DRIVER (Chinese)

127

Electrocardiographic abnormalities and associated factors in Chinese living in Beijing and in Mauritius. The Mauritius Non-Communicable Disease Study Group.

Chonghua, Y.; Chitson, P.; Alberti, K. G.; Li, N.; Fareed, D.; Dowse, G.; Tuomilehto, J.; Zimmet, P.; Gareeboo, H.; Min, Z.

OBJECTIVE--To compare the prevalence of electrocardiographic abnormalities and to evaluate the association between these abnormalities and the levels of coronary heart disease among Chinese living in different environments. DESIGN--Cross sectional surveys. SETTING--Beijing, China, and the island of ...

DRIVER (Chinese)

138

Effect of dietary soybean lecithin on reproductive performance of Chinese mitten crab Eriocheir sinensis (H. Milne-Edwards) broodstock

Sui, L.Y.; Wu, X.G.; Wille, M.; Cheng, Y.X.; Sorgeloos, P.

The effect of increasing levels of dietary phospholipids (PL) on the ovarian development and reproductive performance of Chinese mitten crab Eriocheir sinensis were investigated using four semipurified formulated diets supplemented with 0%, 1.2%, 2.4%, and 3.6% PL. Four groups of 40 females, ...

DRIVER (Chinese)

144

Deregulation of hamster fibroblast proliferation by mutated ras oncogenes is not mediated by constitutive activation of phosphoinositide-specific phospholipase C.

Seuwen, K; Pouysségur, J; Lagarde, A

Stable expression of high levels of activated forms of Haras (T24) or v-Ki-ras by transfection of Chinese hamster lung fibroblasts (CCL39) yielded cells highly tumorigenic in nude mice. Two classes of transformed cells were distinguished, one with moderate p21 expression (10-fold increased) had reta...

DRIVER (Chinese)

145

DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.

Andrulis, I L; Siminovitch, L

Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived ...

DRIVER (Chinese)

146

DNA methods for identification of Chinese medicinal materials

Kwan, Hoi Shan; Yip, Pui Ying; Mak, Chun Yin; Chau, Chi Fai

As adulterated and substituted Chinese medicinal materials are common in the market, therapeutic effectiveness of such materials cannot be guaranteed. Identification at species-, strain- and locality-levels, therefore, is required for quality assurance/control of Chinese medicine. This review provid...

DRIVER (Chinese)

147

Coordinate expression of amplified metallothionein I and II genes in cadmium-resistant Chinese hamster cells.

Griffith, J K

Recombinant DNA probes complementary to Chinese hamster metallothionein (MT)-1 and MT-2 mRNAs were used to compare MT gene copy numbers, zinc-induced MT mRNA levels, and uninduced MT mRNA levels in cadmium-resistant (Cdr) Chinese hamster ovary cell lines. Quantitative hybridization analyses determin...

DRIVER (Chinese)

148

Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.

Anisowicz, A; Sager, R; Bardwell, L

Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology wit...

DRIVER (Chinese)

153

Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.

Gossels, S D; Kaufman, R J; Spiliotes, A J; Wasley, L C; Latt, S A; Larsen, G R; Kay, R M

Expression of human tissue-type plasminogen activator (t-PA) at high levels has been achieved in Chinese hamster ovary (CHO) cells by cotransfection and subsequent coamplification of the transfected sequences. Expression vectors containing the t-PA cDNA gene and dihydrofolate reductase (DHFR) cDNA g...

DRIVER (Chinese)

156

Chinese hamster ovary cell mutants defective in the internalization of ricin.

Ray, B; Wu, H C

Chinese hamster ovary mutants simultaneously resistant to ricin and Pseudomonas toxin have been isolated. Two mutant cell lines (4-10 and 11-2) were found to retain normal levels of binding of both ricin and Pseudomonas toxin. They were defective in the internalization of [125I]ricin into the mutant...

DRIVER (Chinese)

161

China and Vietnam chances and limitations of bilateral cooperation

Will, Gerhard; Bundesinstitut für ostwissenschaftliche und internationale Studien

'Die chinesisch-vietnamesischen Beziehungen bieten seit einigen Jahren ein sehr widersprüchliches Erscheinungsbild. Trotz ständig wiederholter Freundschaftsbeteuerungen, einem dichten Austausch von Delegationen auf allen Ebenen und stark angewachsenen Handelsbeziehungen, beschuldigen sich beide Seit...

DRIVER (Chinese)

163

Cellular and Species Resistance to Murine Amphotropic, Gibbon Ape, and Feline Subgroup C Leukemia Viruses Is Strongly Influenced by Receptor Expression Levels and by Receptor Masking Mechanisms

Tailor, Chetankumar S.; Kabat, David; Nouri, Ali

Chinese hamster ovary (CHO) cells are resistant to infections by gibbon ape leukemia virus (GALV) and amphotropic murine leukemia virus (A-MLV) unless they are pretreated with tunicamycin, an inhibitor of N-linked glycosylation. These viruses use the related sodium-phosphate symporters Pit1 and Pit2...

DRIVER (Chinese)

164

CYP2A6, MAOA, DBH, DRD4, and 5HT2A genotypes, smoking behaviour and cotinine levels in 1518 UK adolescents

Huang, SW; Cook, DG; Hinks, LJ; Chen, XH; Ye, S; Gilg, JA; Jarvis, MJ; Whincup, PH; Day, INM

Objectives Smoking is a major cause of death and often initiates in adolescence. Mutations in CYP2A6 slow metabolism of nicotine to cotinine. Haploinsufficiency in adults is associated with lower cigarette consumption, lower cotinine level and higher quit rates. Other genes are also implicated in sm...

DRIVER (Chinese)

165

Bone turnover markers and prediction of bone loss in elderly women

Lenora, Janaka

Around 70,000 osteoporosis-related fractures occur in Sweden annually and approximately half of the women in western world will sustain a fragility fracture after the age of 50 years. Fracture preventive efforts require the identification of individuals who are at high risk. Biochemical markers of b...

DRIVER (Chinese)

166

Behaviour problems in Chinese primary school children.

Hesketh, T; Zheng, Y; Jun, YX; Xing, ZW; Dong, ZX; Lu, L

PURPOSE: This study aimed to determine levels of behaviour problem in primary school children, and to explore key determinants relevant to the Chinese context: being an only child, urban living, school stressors, being bullied and physical punishment. METHODS: We administered a child self-completion...

DRIVER (Chinese)

168

Association of antihypertensive monotherapy with serum sodium and potassium levels in Chinese patients

Jiang, J.Y.; Wong, M.C.; Ali, M.K.; Griffiths, S.; Mercer, S.W.

Background: International guidelines on management of hypertension recommend any major classes of antihypertensive drugs. However, the low prescribing rate of thiazides has been attributed to concerns about electrolyte disturbances and studies between antihypertensive drug classes and hyponat...

DRIVER (Chinese)

169

Assessment of toxicity of heavy metal contaminated soils for Collembola in the field and laboratory

Xu, Jie; Krogh, Paul Henning; Luo, Yongming; Song, Jing; Wang, Yin; Gao, Yan; Ke, Xin

We present a field and laboratory investigation of effects of increasing levels of heavy metal contamination on the biodiversity and performance of collembolans. A 40 year old pollution with Cu, Zn, Pb and Cd pollution due to Cu smelting over 40 years was investigated in a paddy field area of Zhejia...

DRIVER (Chinese)

170

Apolipoprotein(a) phenotypes, Lp(a) concentration and plasma lipid levels in relation to coronary heart disease in a Chinese population: evidence for the role of the apo(a) gene in coronary heart disease.

Tong, M C; Sandholzer, C; Saha, N; Boerwinkle, E; Utermann, G

Elevated lipoprotein(a) (Lp[a]) concentrations are associated with premature coronary heart disease (CHD). In the general population, Lp(a) levels are largely determined by alleles at the hypervariable apolipoprotein(a) (apo[a]) gene locus, but other genetic and environmental factors also affect pla...

DRIVER (Chinese)

171

Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.

Urlaub, G; Nunberg, J H; Schimke, R T; Kaufman, R J; Chasin, L A

Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate: NADP+ oxidoreductase, EC 1.5.1.3) 200 times that of sensitive cel...

DRIVER (Chinese)

172

Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.

Kaufman, R J; Schimke, R T

During stepwise increases in the methotrexate concentration in culture medium, we selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional to the number of dihydrofolate reductase gene copies (i.e., gene amplification). We studied the dihydr...

DRIVER (Chinese)

173

Active immunization against gonadotrophin-releasing hormone in Chinese male pigs: effects of dose on antibody titer, hormone levels and sexual development

Zeng, X.Y.; Turkstra, J.A.; Meloen, R.H.; Liu, X.Y.; Chen, F.Q.; Schaaper, W.M.M.; Oonk, H.B.; Guo, D.Z.; Wiel, D.F.M., van de

The objective of this study was to determine the optimal dose of a GnRH vaccine for immunocastration of Chinese male pigs, based on immune, endocrine and testicular responses. Forty-two crossbred (Chinese Yanan x Large White) male pigs were randomly assigned to one of the five treatments as follows:...

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174

Aberrante Methylierung des p16INK4a-Promotors als Tumormarker für die molekularzytologische Diagnostik des Lungenkarzinoms an Bronchialaspiraten

Krücken, Anne

In der vorliegenden Arbeit wurde der Methylierungsstatus des p16INK4a-Promotors an Material der pulmonalen Exfoliativzytologie untersucht und seine Eignung als Tumormarker für die molekularzytologische Diagnostik des Lungenkarzinoms überprüft. Der Nachweis der p16INK4a-Promotor-Methylierung erfolgte...

DRIVER (Chinese)

175

A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells.

Mayr, G A; Freimuth, P

The receptors on human cells which mediate adsorption of adenoviruses have not been identified. We found that murine A9 cells and Chinese hamster ovary (CHO) cells failed to bind significant levels of radiolabeled adenovirus type 2 (Ad2) virions but that derivatives of these cells carrying human chr...

DRIVER (Chinese)

176

A novel DNA damage-inducible transcript, gadd7, inhibits cell growth, but lacks a protein product.

Hollander, M C; Fornace, A J; Alamo, I

gadd7 cDNA was isolated from Chinese hamster ovary (CHO) cells on the basis of increased levels of RNA following treatment with UV radiation. The transcript for gadd7, as well as for four other gadd genes, was found to increase rapidly and coordinately following several different types of DNA damage...

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