Sample records for 调查 (investigations)

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Xia, Dunsheng(夏敦胜); Chen, Fahu(陈发虎); Ma, Jianying(马剑英); Liu, Xiuming(刘秀铭); Zhang, Weiguo(张卫国); Wang, Xunming(王训明)

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Xiaoren, Yang

This paper gives some specific features and the evaluation results of the Chinese primary frequency standards. Some investigations on the frequency shifts due to the Millman effect and the Majorana effect are discussed.

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Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

Cho, Seungho; Muehlbauer, Gary J.; Garvin, David F.

Wheat–barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese Spring–Betzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe array. Of...

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The in Silico Map-Based Cloning of Pi36, a Rice Coiled-Coil–Nucleotide-Binding Site–Leucine-Rich Repeat Gene That Confers Race-Specific Resistance to the Blast Fungus

Pan, Qinghua; Liu, Xinqiong; Wang, Ling; Lin, Fei

The indica rice variety Kasalath carries Pi36, a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8. The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance (...

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Temperature-Sensitive Mutants of a Chinese Hamster Cell Line. I. Selection of Clones with Defective Macromolecular Biosynthesis

Roufa, Donald J.; Reed, Susan J.

Temperature-sensitive clones have been selected from a mutagenized culture of Chinese hamster lung cells by a procedure involving bromodeoxy-uridine (BrdU) incorporation and irradiation with black light. The selection procedure used in these studies was adapted from methods developed by others to yi...

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FANGChuang-lin; HUANGJin-chuan; 等

Based on the field investigations,this paper analyzes systematically the achievements and problems of constructing the demonstrating areas of the ecological rebuilding and economic sustainable development in Hexi,Gansu Province,and then the thoughts on constructing the demonstrating areas are brough...

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Separation of Linked Markers in Chinese Hamster Cell Hybrids: Mitotic Recombination Is Not Involved

Rosenstraus, Maurice J.; Chasin, Lawrence A.

A search for mitotic recombination was carried out using mutant subclones of cultured Chinese hamster ovary cells. Recombination events were sought between the linked loci specifying the enzymes hypoxanthine phosphoribosyl transferase and glucose-6-phosphate dehydrogenase. It was shown by fluctuatio...

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Requirement for Cell Dispersion Prior to Selection of Induced Azaguanine-Resistant Colonies of Chinese Hamster Cells

Myhr, B. C.; DiPaolo, J. A.

With V79 Chinese hamster cell cultures treated with a mutagen, the maximum frequency of colonies resistant to 8-azaguanine (AZG) was attained when the cells were dispersed after a suitable expression time before adding the selection medium. V79–4 cells were exposed to 500 µM MMS, 7 µM AFAA, or 10 µM...

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Preliminary research on Megalake Jilantai-Hetao in the arid areas of China during the Late Quaternary

Chen, Fahu; Fan, Yuxin; Chun, Xi; MADSEN, D.B.; OVIATT, C.G.; Zhao, Hui; Yang, Liping; Sun, Yang

We identified geomorphological, sedimentary and biological evidence of high lake levels around the current Jilantai Salt Lake through field investigations and through analyses of regional remote sensing images. There are four groups of shorelines at elevations of around 1060, 1050, 1044 and 1035 m a...

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Nonstructural Chromosome Differentiation among Wheat Cultivars, with Special Reference to Differentiation of Chromosomes in Related Species

Dvořák, Jan; McGuire, Patrick E.

Wheat cultivar Chinese Spring (Triticum aestivum L. em. Thell.) was crossed with cultivars Hope, Cheyenne and Timstein. In all three hybrids, the frequencies of pollen mother cells (PMCs) with univalents at metaphase I (MI) were higher than those in the parental cultivars. No multivalents were obser...

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Mutations of Chinese Hamster Somatic Cells from 2-Deoxygalactose Sensitivity to Resistance

Claverie, Jean-Michel; Thirion, Jean-Paul; De Souza, Aristide Comlan

In Chinese hamster somatic cells, the spontaneous change of phenotype from 2-deoxygalactose sensitivity to resistance was studied using fluctuation test experiments à la Luria and Delbrück (1943) for four Chinese hamster cell strains derived from V79. The results are consistent with true mutational ...

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Mismatch Repair by Efficient Nick-Directed, and Less Efficient Mismatch-Specific, Mechanisms in Homologous Recombination Intermediates in Chinese Hamster Ovary Cells

Nickoloff, J. A.; Miller, E. M.; Cho, J. W.; Hough, H. L.

Repair of single-base mismatches formed in recombination intermediates in vivo was investigated in Chinese hamster ovary cells. Extrachromosomal recombination was stimulated by double-strand breaks (DSBs) introduced into regions of shared homology in pairs of plasmid substrates heteroallelic at 11 p...

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Induction of Thioguanine- and Ouabain-Resistant Mutants and Single-Strand Breaks in the DNA of Chinese Hamster Ovary Cells by 3H-Thymidine

Cleaver, James E.

Cultured Chinese hamster cells were labeled with 6-3H-thymidine or 5-methyl-3H-thymidine and allowed to accumulate damage from 3H decays for various periods of time while frozen. The frequencies of cells resistant to 6-thioguanine or ouabain and the amount of DNA damage (i.e., number of single-stran...

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Human Population Genetic Studies Using Hypervariable Loci. I. Analysis of Assamese, Australian, Cambodian, Caucasian, Chinese and Melanesian Populations

Kidd, J.; Balazs, I.; Gunn, P.; Neuweiler, J.; Kidd, K. K.; Kuhl, J.; Mingjun, L.

Population genetic studies, in Australian, Assamese, Cambodian, Chinese, Caucasian and Melanesian populations, were performed with several highly polymorphic DNA loci. Results showed that the Caucasian and Chinese had the highest level of heterozygosity. The size range of the majority of the polymor...

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HLA class II alleles in Chinese patients with hepatocellular carcinoma

Donaldson, PT; Ho, S; Williams, R; Johnson, PJ

BACKGROUND/AIMS: Recent reports of an association between human leucocyte antigens (HLA) and persistence of hepatitis B virus infection, and the familial clustering of hepatocellular carcinoma raise the question of genetic susceptibility. Previous studies have been limited to serological phenotyping...

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HKU Tibet Research Group: Results of five years of investigations along the Yarlung Tsangpo suture zone

Aitchison, JC; Abrajevitch, A; Ali, JR; Badengzhu,; Davis, AM; Luo, H; Liu, J; McDermid, IRC; Ziabrev, S

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Genomic Instability in Wheat Induced by Chromosome 6b(s) of Triticum Speltoides

Kota, R. S.; Dvorak, J.

A massive restructuring of chromosomes was observed during the production of a substitution of chromosome 6B(s) from Triticum speltoides (Tausch) Gren. ex Richter for chromosome 6B of Chinese Spring wheat (Triticum aestivum L.). Deletions, translocations, ring chromosomes, dicentric chromosomes and ...

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Galactokinase Mutants of Chinese Hamster Somatic Cells Resistant to 2-Deoxygalactose

Thirion, Jean-Paul; Noel, Henri; Banville, Denis

The growth of Chinese hamster somatic cells was inhibited by 0.2 mg/cc of 2-deoxygalactose. Mutants partially or fully resistant to 2-deoxygalactose were isolated in a single-step or two-step selection. Some of them did not grow as well as the wild type; one of them which lacked galactokinase (EC.2....

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Fine-Scale Map of Encyclopedia of DNA Elements Regions in the Korean Population

Song, Okryeol; Lee, Yeun-Du; Kim, Young J.; Lee, Jong-Eun; Ahn, TaeJin; Rho, Na-Young; Lee, Yeon-Su; Hong, Sungwoo

The International HapMap Project aims to generate detailed human genome variation maps by densely genotyping single-nucleotide polymorphisms (SNPs) in CEPH, Chinese, Japanese, and Yoruba samples. This will undoubtedly become an important facility for genetic studies of diseases and complex traits in...

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Epstein syndrome presenting as renal failure in young patients

Yap, DYH; Tse, KC; Chan, TM; Lie, AKW

Two young Chinese patients presented with renal failure and thrombocytopenia. Further investigations showed the presence of large platelets and high-frequency sensorineural hearing deficit. Genetic studies confirmed mutations in the gene encoding the myosin heavy chain (MYH-9), and Epstein Syndrome ...

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Distribution of Nonstructural Variation along Three Chromosome Arms between Wheat Cultivars Chinese Spring and Cheyenne

Crossway, Anne; Dvořák, Jan

Metaphase I (MI) pairing of wheat homologous chromosomes is usually reduced in hybrids between cultivars relative to the parental inbred lines. Previous work suggested that this phenomenon is caused by polymorphism in nucleotide sequences (nonstructural chromosome variation) among wheat cultivars. T...

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Derivation of Tk- Clones from Revertant Tk+ Mammalian Cells

Roufa, D. J.; Caskey, C. T.; Sadow, B. N.

In order to obtain a large collection of Chinese hamster cell clones defective in thymidine kinase (TK-), BrdUr selection experiments have been performed on wild-type and revertant TK+ cell lines. No clones (< 10-9) were obtained from the wild-type TK+ cell line by single-step selection. In contr...

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Control of Endosperm Proteins in TRITICUM AESTIVUM (Var. Chinese Spring) and AEGILOPS UMBELLULATA by Homoeologous Group 1 Chromosomes

Flavell, Richard B.; Brown, John W. S.; Law, Colin N.; Kemble, Roger J.

The genetic control of major wheat endosperm proteins by homoeologous group 1 chromosomes has been studied by two-dimensional polyacrylamide gel electrophoresis. The control of at least 15 distinct protein subunits or groups of protein subunits has been allocated to chromosomes 1A, 1B and 1D of Chin...

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Construction and Evaluation of cDNA Libraries for Large-Scale Expressed Sequence Tag Sequencing in Wheat (Triticum aestivum L.)

Fenton, R. D.; Kianian, P.; Simons, K.; Laudencia-Chingcuanco, D.; Crossman, C. C.; Miller, R. E.; Close, T. J.; Malatrasi, M.

A total of 37 original cDNA libraries and 9 derivative libraries enriched for rare sequences were produced from Chinese Spring wheat (Triticum aestivum L.), five other hexaploid wheat genotypes (Cheyenne, Brevor, TAM W101, BH1146, Butte 86), tetraploid durum wheat (T. turgidum L.), diploid wheat (T....

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Chromosomal Location of Genes Controlling Flavonoid Production in Hexaploid Wheat

Barnhart, D.; Neuman, Paula R.; Hilu, K. W.; Waines, J. G.

Two-dimensional paper chromatography was performed on methanol extracts of leaves of hexaploid bread wheat, Triticum aestivum L. em. Thell. cultivar Chinese Spring, and of the available nullisomic-tetrasomic compensating lines, the tetrasomic lines and the ditelocentric lines. The chromatograms had ...

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Bromodeoxyuridine-Induced Mutations in Synchronous Chinese Hamster Cells: Temporal Induction of 6-Thioguanine and Ouabain Resistance during DNA Replication

Burki, H. John; Aebersold, Paul M.

Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguani...

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Berberine and Coptidis rhizoma as novel antineoplastic agents: a review of traditional use and biomedical investigations

Tang, J; Feng, Y; Tsao, GSW; Wang, N; Curtain, RP; Wang, Y

ETHNOPHARMACOLOGICAL RELEVANCE: Coptidis rhizoma (huanglian) and its major component, berberine, have drawn extensive attention toward their antineoplastic effects in the recent years. The antineoplastic effects are related to the Chinese Medicine (CM) properties of huanglian in treating diseases by...

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Bear bile: dilemma of traditional medicinal use and animal protection

Feng, Y; Siu, K; Wang, N; Ng, KM; Tsao, SW; Nagamatsu, T; Tong, Y

Bear bile has been used in Traditional Chinese Medicine (TCM) for thousands of years. Modern investigations showed that it has a wide range of pharmacological actions with little toxicological side effect and the pure compounds have been used for curing hepatic and biliary disorders for decades. How...

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Bayesian Statistical Analyses for Presence of Single Genes Affecting Meat Quality Traits in a Crossed Pig Population

Janss, LLG.; Brascamp, E. W.; Van-Arendonk, JAM.

Presence of single genes affecting meat quality traits was investigated in F(2) individuals of a cross between Chinese Meishan and Western pig lines using phenotypic measurements on 11 traits. A Bayesian approach was used for inference about a mixed model of inheritance, postulating effects of polyg...

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Alcohol Dehydrogenase Mutants of Chinese Hamster Somatic Cells Resistant to Allyl Alcohol

Thirion, Jean-Paul; Talbot, Brian

Alcohol dehydrogenase (alcohol: NAD oxidoreductase, E.C. mutants of Chinese hamster somatic cells were isolated as resistant to allyl alcohol (ALLR). The ALLR phenotypes of the mutant clones were reproducible with high fidelity and stable over long intervals of growth in the absence of the...

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A Role for DNA Mismatch Repair Protein Msh2 in Error-Prone Double-Strand-Break Repair in Mammalian Chromosomes

Smith, Jason A.; Waldman, Alan S.; Waldman, Barbara Criscuolo

We examined error-prone nonhomologous end joining (NHEJ) in Msh2-deficient and wild-type Chinese hamster ovary cell lines. A DNA substrate containing a thymidine kinase (tk) gene fused to a neomycin-resistance (neo) gene was stably integrated into cells. The fusion gene was rendered nonfunctional du...

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8-Azaguanine Resistance in Mammalian Cells I. Hypoxanthine-Guanine Phosphoribosyltransferase

Caskey, C. T.; Gillin, F. D.; Beaudet, A. L.; Roufa, D. J.

Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for re...

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