Sample records for 干扰素 (interferon)
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17

The chromosomal location of mouse interferon alpha genes.

Boll, W; Lovett, M; Yee, D; Cox, D R; Weissmann, C; Epstein, C J; Epstein, L B

The chromosomal location of mouse leukocyte-interferon (IFN-alpha) genes was determined by Southern blot analysis of DNA from a panel of Chinese hamster x mouse somatic cell hybrids using a mouse IFN-alpha cDNA as a hybridization probe. All resolvable mouse genes are located on mouse chromosome 4. I...

DRIVER (Chinese)

20

Posttranslational processing of recombinant human interferon-gamma in animal expression systems.

Jenkins, N.; James, D. C.; Hoare, M.; Goldman, M. H.; Oliver, R. W.; Green, B. N.; Freedman, R. B.

We have characterized the heterogeneity of recombinant human interferon-gamma (IFN-gamma) produced by three expression systems: Chinese hamster ovary cells, the mammary gland of transgenic mice, and baculovirus-infected Spodopera frugiperda (Sf9) insect cells. Analyses of whole IFN-gamma proteins by...

DRIVER (Chinese)

21

Posttranslational processing of recombinant human interferon-gamma in animal expression systems

James, DC; Goldman, MH; Hoare, M; Jenkins, N; Oliver, RWA; Green, BN; Freedman, RB

We have characterized the heterogeneity of recombinant human interferon-gamma (IFN-gamma) produced by three expression systems: Chinese hamster ovary cells, the mammary gland of transgenic mice, and baculovirus-infected Spodoptera frugiperda (Sf9) insect cells. Analyses of whole IFN-gamma proteins b...

DRIVER (Chinese)

22

N-GLYCOSYLATION OF RECOMBINANT HUMAN INTERFERON-GAMMA PRODUCED IN DIFFERENT ANIMAL EXPRESSION SYSTEMS

JAMES, DC; FREEDMAN, RB; HOARE, M; OGONAH, OW; ROONEY, BC; LARIONOV, OA; DOBROVOLSKY, VN; LAGUTIN, OV; JENKINS, N

Recombinant human interferon-gamma (IFN-gamma) was expressed in Chinese hamster ovary cells, baculovirus-infected Sf9 insect cells and the mammary gland of transgenic mice, The N-linked carbohydrate populations associated with both Asn(25), and Asn(97) glycosylation sites were characterized by matri...

DRIVER (Chinese)

24

Monitoring recombinant human interferon-gamma n-glycosylation during perfused fluidized-bed and stirred-tank batch culture of CHO cells

Goldman, MH; James, DC; Rendall, M; Ison, AP; Hoare, M; Bulll, AT

Chinese hamster ovary cells producing recombinant human interferon-gamma were cultivated for 500 h attached to macroporous microcarriers in a perfused, fluidized-bed bioreactor, reaching a maximum cell density in excess of 3 x 10(7) cells (mL microcarrier)(-1) at a specific growth rate (mu) of 0.010...

DRIVER (Chinese)

28

Inhibition of protein synthesis stimulates the transcription of human beta-interferon genes in Chinese hamster ovary cells.

Trahey, M; Ringold, G M; Vannice, J L; Dieckmann, B; McCormick, F

Using Chinese hamster ovary (CHO) cells transfected with a plasmid carrying the human beta-interferon gene, we find that inhibitors of protein synthesis, in the absence of any other inducer, stimulate the production of interferon RNA; this effect is maintained in cells in which the plasmid sequences...

DRIVER (Chinese)

29

Inducible expression of amplified human beta interferon genes in CHO cells.

Dieckmann, B; McCormick, F; Innis, M; Trahey, M; Ringold, G

Plasmid DNA containing the human beta-interferon (IFN-beta) gene and mouse dihydrofolate reductase cDNA was transfected into dihydrofolate reductase-negative Chinese hamster ovary cells. Dihydrofolate reductase-positive transformants were obtained, and cells containing amplified copies of mouse dihy...

DRIVER (Chinese)

36

Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.

Fiers, W; Scahill, S J; Van der Heyden, J; Devos, R

Cotransformation with two plasmids, one [pSV2-IFN-gamma] encoding human immune interferon (Hu IFN-gamma) and the other [pAdD26SV(A)-3] encoding mouse dihydrofolate reductase, has been used to establish Chinese hamster ovary (CHO) cell lines that secrete high levels of Hu IFN-gamma. Hu IFN-gamma prod...

DRIVER (Chinese)