Sample records for 胰岛素 (insulin)

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Validation of Bioelectrical Impedance Analysis in Adolescents Across Different Ethnic Groups

Haroun, D; Taylor, SJC; Viner, RM; Hayward, RS; Darch, TS; Eaton, S; Cole, TJ; Wells, JCK

Adolescent obesity is difficult to assess in multi-ethnic populations using BMI, due to variability in the BMI-fatness relationship. We aimed to describe body composition (BC), and to validate leg-leg bioelectrical impedance analysis (BIA), in adolescents from different ethnic groups using deuterium...

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Two dominant inhibitory mutants of p21ras interfere with insulin-induced gene expression.

Medema, R H; Bos, J L; Wubbolts, R

Insulin induces a rapid activation of p21ras in NIH 3T3 and Chinese hamster ovary cells that overexpress the insulin receptor. Previously, we suggested that p21ras may mediate insulin-induced gene expression. To test such a function of p21ras more directly, we studied the effect of different dominan...

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Two dominant inhibitory mutants of p21ras interfere with insulin-induced gene expression

Medema, R.H.; Wubbolts, R.; Bos, J.L.

Insulin induces a rapid activation of p21ras in NIH 3T3 and Chinese hamster ovary cells that overexpress the insulin receptor. Previously, we suggested that p21ras may mediate insulin-induced gene expression. To test such a function of p21ras more directly, we studied the effect of different dominan...

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The metabolic syndrome is associated with subclinical atherosclerosis independent of insulin resistance: the Guangzhou Biobank Cohort Study-CVD

Xu, L; Jiang, CQ; Lam, TH; Lin, JM; Yue, XJ; Cheng, KK; Liu, B; Jin, YL; Zhang, WS; Thomas, GN

OBJECTIVE: We examined whether the association of the metabolic syndrome (MetS) and subclinical atherosclerosis is independent of insulin resistance in a Chinese community sample with no history of type 2 diabetes. METHODS: Five hundred and ninety-six men and 526 women from a substudy of the Guangzh...

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The insulin receptor as a transmitter of a mitogenic signal in Chinese hamster ovary CHO-K1 cells.

Mamounas, M; Englesberg, E; Gervin, D

Insulin is the only hormone required for continued growth of Chinese hamster ovary CHO-K1 cells in the defined medium M-F12. When CHO-K1 cells are incubated in M-F12 without insulin for 48-72 hr, the cells accumulate in G1. In response to physiological concentrations of insulin an 18-fold increase i...

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Replacement of insulin receptor tyrosine residues 1162 and 1163 does not alter the mitogenic effect of the hormone.

Filloux, C; Debant, A; Ponzio, G; Clauser, E; Auzan, C; Contreres, J O; Rossi, B

Chinese hamster ovary transfectants that express insulin receptors in which tyrosine residues 1162 and 1163 were replaced by phenylalanine exhibit a total inhibition of the insulin-mediated tyrosine kinase activity toward exogenous substrates [histone, casein, and poly(Glu/Tyr)]; this latter activit...

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Protein-free cell culture on an artificial substrate with covalently immobilized insulin.

Yonezawa, K; Ito, Y; Imanishi, Y; Zheng, J; Kasuga, M

Insulin was immobilized on a surface-hydrolyzed poly(methyl methacrylate) film. Chinese hamster ovary cells overexpressing human insulin receptors were cultured on the film in the absence of serum or soluble proteins. Small amounts of immobilized insulin (1-10% of the required amount of free insulin...

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Pre-adipocyte determination either by insulin or by 5-azacytidine.

Sager, R; Kovac, P

CHEF/18 is a diploid Chinese hamster cell line of embryonic origin, which is fibroblastic in structure, but behaves like a mesenchymal stem cell line in its ability to differentiate into adipocytes, myoblasts, and chondrocytes. With these cells, adipocyte formation has been divided experimentally in...

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Phosphatidylinositol 3,4,5-trisphosphate modulation in Ship2-deficient mouse embryonic fibroblasts

Blero, D; Zhang, J; Pesesse, X; Payrastre, B; Dumont, JE; Schurmans, Stéphane; Erneux, C

SHIP2, the ubiquitous SH2 domain containing inositol 5-phosphatase, includes a series of protein interacting domains and has the ability to dephosphorylate phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3] in vitro. The present study, which was undertaken to evaluate the impact of SHIP2 on ...

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Mutation of tyrosine-141 inhibits insulin-promoted tyrosine phosphorylation and increased responsiveness of the human beta 2-adrenergic receptor.

Bouvier, M; Valiquette, M; Loisel, T P; Parent, S

The ability of insulin to promote phosphorylation of the human beta 2-adrenergic receptor (beta 2AR) was assessed in Chinese hamster fibroblasts transfected with beta 2AR cDNA. Phosphotyrosine residues were detected in purified beta 2AR using a polyclonal anti-phosphotyrosine antibody and by phospho...

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Linkage disequilibrium in the human insulin/insulin-like growth factor II region of human chromosome II.

Cox, N J; Xiang, K S; Bell, G I

Caucasian (N = 128) and Chinese (N = 84) subjects were typed for RFLPs in the insulin (INS)/insulin-like growth factor II (IGF2) region of chromosome 11. Both the analysis of extended haplotypes and the pairwise measures of linkage disequilibrium among the RFLPs indicate that there is extensive link...

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Insulin-stimulated disassociation of the SOS-Grb2 complex.

Waters, S B; Pessin, J E; Yamauchi, K

Insulin stimulation of differentiated 3T3-L1 adipocytes or Chinese hamster ovary cells expressing high levels of the insulin receptor resulted in a time-dependent decrease in the electrophoretic mobility of SOS on sodium dodecyl sulfate-polyacrylamide gels. The reduction in SOS mobility was complete...

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Insulin-induced surface redistribution regulates internalization of the insulin receptor and requires its autophosphorylation.

Rutter, W J; Carpentier, J L; Gorden, P; Paccaud, J P; Orci, L

The role of insulin-induced receptor autophosphorylation in its internalization was analyzed by comparing 125I-labeled insulin (125I-insulin) internalization in Chinese hamster ovary (CHO) cell lines transfected with normal (CHO.T) or mutated insulin receptors. In four cell lines with a defect of in...

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Insulin regulates the dynamic balance between Ras and Rap1 signaling by coordinating the assembly states of the Grb2-SOS and CrkII-C3G complexes.

Pawson, T; Okada, S; Anafi, M; Matsuda, M; Pessin, J E

Insulin stimulation of Chinese hamster ovary cells expressing the human insulin receptor resulted in a time-dependent decrease in the amount of GTP bound to Rap1. The inactivation of Rap1 was associated with an insulin-stimulated decrease in the amount of Rap1 that was bound to Raf1. In parallel wit...

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Insulin binding and insulin action in cultured fibroblasts: significant differences between a phosphoglucose isomerase-deficient mutant and the parental strain.

Kalckar, H M; Wilson, C; Ullrey, D B; Peterson, S W

The interrelationships of hexose feeding and insulin action were studied in the Chinese hamster fibroblast cell lines 023 and DS-7. The latter, derived from 023 and deficient in phosphoglucose isomerase, has been used to map the metabolic requirements for aldohexose-mediated down-regulation or "curb...

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Heterologous transmembrane signaling by a human insulin receptor-v-ros hybrid in Chinese hamster ovary cells.

Wang, L H; Ellis, L; Jong, S M; Morgan, D O; Roth, R A; Rutter, W J

A hybrid receptor molecule composed of the extracellular ligand-binding domain of the human insulin receptor and the transmembrane and cytoplasmic (protein-tyrosine kinase) domains of the chicken sarcoma virus UR2 transforming protein p68gag-ros has been constructed and expressed in Chinese hamster ...

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Haplotypes in the urotensin II gene and urotensin II receptor gene are associated with insulin resistance and impaired glucose tolerance

Ong, KL; Wong, LYF; Man, YB; Leung, Y; Song, Y; Lam, KSL; Cheung, BMY

We studied single nucleotide polymorphisms (SNPs) and haplotypes in the urotensin-II (UTS2) and urotensin-II receptor gene (UTS2R) in Hong Kong Chinese (224 hypertensive and 306 normotensive unrelated subjects) and their relation to hypertension and the metabolic syndrome. For UTS2, the GGT haplotyp...

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Growth and morphology of colonies of Chinese hamster ovary cells growing on agar is affected by insulin

Aidells, Bruce D.; Glaser, Donald A.; Konrad, Michael W.

As a model for the effect of hormones and growth factors on three-dimensional growth of mammalian cells, we have analyzed the effect of insulin on the three-dimensional growth and morphology of Chinese hamster ovary (CHO) colonies grown on the surface of agar. Sequential photographs in dark-field il...

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Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.

Standring, D; Ebina, Y; Ellis, L; Edery, M; Beaudoin, J; Roth, R A; Rutter, W J

We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus oocytes and by expression in stably transformed CHO cells. The precursor and the alpha and beta subunits of...

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Enhancement or inhibition of insulin signaling by insulin receptor substrate 1 is cell context dependent.

Yamauchi, K; Pessin, J E

Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin...

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ENPP1 K121Q Polymorphism is not Related to Type 2 Diabetes Mellitus, Features of Metabolic Syndrome, and Diabetic Cardiovascular Complications in a Chinese Population

Tsai, Jack C-R; Chen, Miao-Pei; Chang, Dao-Ming; Chung, Fu-Mei; Huang, Han-Fen; Shin, Shyi-Jang; Lee, Yau-Jiunn

BACKGROUND: Ectoenzyme nucleotide pyrophosphate phosphodiesterase 1 (ENPP1) is known to influence insulin sensitivity by inhibiting insulin receptor signaling. A DNA polymorphism in the ENPP1 gene at exon 4 (K121Q) was demonstrated to be associated with insulin resistance, type 2 diabetes mellitus (...

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Control of A-system amino acid transport by a second regulatory gene R2 in Chinese hamster ovary cells CHO-K1 and the possible connection of this gene with insulin activity.

Englesberg, E; Moffett, J; Jones, M; Périer, F

Evidence based on a study of alanine-resistant (Alar), constitutive mutants of CHO-K1 cells and the conditions that favor stimulation of the A system of amino acid activity supports the model that the A system of amino acid transport in these cells is repressible and under negative control of regula...

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Analysis of the genes involved in the insulin transmembrane mitogenic signal in Chinese hamster ovary cells, CHO-K1, utilizing insulin-independent mutants.

Brown, E; Mamounas, M; Luong, C L; Ross, S; Coulter, K; Carroll, G; Englesberg, E

CHO-K1 cells, wild type (WT), grow in a defined medium with insulin as the only essential hormone. When starved for insulin, these cells accumulate in G0/G1 stage. Insulin binding to its receptor stimulates DNA synthesis and cell division and induces an increase in abundance of mRNA for c-fos, c-jun...

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Analysis of Insulin-Gene Linked Polymorphic Region (ILPR) in Chinese IDDM Patients

Cheung, PT; Low, LCK; Hawkins, BR; Tam, KCB; Lam, KSL; Kwan, EYW

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Abnormal Secretion of Insulin and Glucagon by the In Vitro Perfused Pancreas of the Genetically Diabetic Chinese Hamster

Fanska, Rudy E.; Frankel, Barbara J.; Hagura, Ryoko; Gerich, John E.; Gerritsen, George C.; Grodsky, Gerold M.

Hereditary insulin-deficient diabetes mellitus occurs in certain sublines of nonobese Chinese hamsters. Several characteristics of this syndrome are similar to those seen in insulin-deficient human diabetics. Therefore, to characterize pancreatic islet function, dynamic insulin and glucagon release ...

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Tala, Hazel Marie Bituin Linan

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Man, Elim; 文爾琳

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Man, Elim; 文爾琳

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