Sample records for 感应 (induction)

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Translocation of ExsE into Chinese Hamster Ovary Cells Is Required for Transcriptional Induction of the Pseudomonas aeruginosa Type III Secretion System▿

Urbanowski, Mark L.; Yahr, Timothy L.; Brutinel, Evan D.

Transcription of the Pseudomonas aeruginosa type III secretion system (T3SS) is induced under Ca2+-limiting growth conditions or following the contact of the bacteria with host cells. The regulatory response to low Ca2+ levels is initiated by the T3SS-mediated secretion of ExsE, a negative regulator...

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Senkyunolide isomers H and I from Rhizoma Chuanxiong attenuate hydrogen peroxide-induced oxidative stress in human liver HepG2 cells via induction of heme oxygenase-1

Qi, H; Siu, SO; Chen, Y; Han, YF; Chu, IK; Tong, Y; Lau, ASY; Rong, J

Biological Activities and Mechanism Study II (Metabolic, neural diseases & Aging process) Session: No. 035

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Persistent DNA damage inhibits S-phase and G2 progression, and results in apoptosis.

Orren, D K; Bohr, V A; Petersen, L N

We used genetically related Chinese hamster ovary cell lines proficient or deficient in DNA repair to determine the direct role of UV-induced DNA photoproducts in inhibition of DNA replication and in induction of G2 arrest and apoptosis. UV irradiation of S-phase-synchronized cells causes delays in ...

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Overexpression of GRP78 mitigates stress induction of glucose regulated proteins and blocks secretion of selective proteins in Chinese hamster ovary cells.

Dorner, A J; Kaufman, R J; Wasley, L C

GRP78 is a resident protein of the endoplasmic reticulum (ER) and a member of the glucose regulated protein (GRP) family. Many secretion incompetent proteins are found in stable association with GRP78 and are retained in the ER. Some proteins which are destined for secretion transiently associate wi...

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On Microvascular Inflammation and Intestinal Leakage in Radiation Enteropathy

Röme, Andrada

Gastrointestinal tract damage is an insidious feature in patients undergoing radiotherapy. Microvascular inflammation, including leukocyte and platelet recruitment as well as epithelial barrier dysfunction, are considered to constitute key components in the pathophysiology of radiation-induced enter...

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Influence of Cultural Conditions on Mitomycin C-Mediated Bacteriophage Induction and Release of Salmonella Toxin

Peterson, Johnny W.; Koo, Felix C. W.; Houston, Clifford W.

Several isolates of Salmonella were examined for the capacity to synthesize and release a cholera toxin-like toxin that exerted a biological effect on Chinese hamster ovary cells. Measurements of this Salmonella toxin, which was contained in cell sonic extracts and culture filtrates, were expressed ...

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Induction of trehalase in Arabidopsis plants infected with the trehalose-producing pathogen Plasmodiophora brassicae

Brodmann, D; Schuller, A; Ludwig-Muller, J; Aeschbacher, RA; Wiemken, A; Boller, T; Wingler, A

Various microorganisms produce the disaccharide trehalose during their symbiotic and pathogenic interactions with plants. Trehalose has strong effects on plant metabolism and growth; therefore, we became interested to study its possible role in the interaction of Arabidopsis thaliana with Plasmodiop...

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Induction of ornithine decarboxylase activity in a temperature-sensitive cell cycle mutant of Chinese hamster cells.

Landy-Otsuka, F; Scheffler, I E

We have investigated the induction of ornithine decarboxylase (L-ornithine carboxy-lyase, EC activity in a temperature-sensitive cell cycle mutant of Chinese hamster fibroblasts. This activity is not induced at the nonpermissive temperature, although the synthesis of the majority of protei...

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Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis.

Xu, J; Manning, F C; Wise, J P; Blankenship, L J; Bridgewater, L C; Patierno, S R

Hexavalent chromium (Cr) compounds are respiratory carcinogens in humans and animals. Treatment of Chinese hamster ovary cells with 150 and 300 microM sodium chromate (Na2CrO4) for 2 hr decreased colony-forming efficiency by 46 and 92%, respectively. These treatments induced dose-dependent internucl...

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Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation.

Sciandra, J J; Hughes, C S; Subjeck, J R

In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environmen...

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Induction of cleavage in topoisomerase I c-DNA by topoisomerase I enzymes from calf thymus and wheat germ in the presence and absence of camptothecin.

Tanizawa, A; Pommier, Y; Kohn, K W

In this study, we further examined the sequence selectivity of camptothecin in mammalian topoisomerase I cDNA from human and Chinese hamster. In the absence of camptothecin, almost all the bases at the 3'-terminus of cleavage sites are T for calf thymus and wheat germ topoisomerase I. In addition, w...

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Induction of chromosome shattering by ultraviolet light and caffeine: The influence of different distributions of photolesions

Cremer, Christoph; Cremer, Thomas

Cells of synchonized and of asynchronously growing cultures of a V79 Chinese hamster line were microirradiated with a low poweer laser-UV-microbeam of wavelength 257 nm. Ultraviolet light was either focused onto a small part of the nucleus (mode I) or distributed over the whole nucleus (mode II). Fo...

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Induction of chromosome shattering by ultraviolet light and caffeine

Cremer, Christoph; Cremer, Thomas; Zorn, Christian; Zimmer, J.

Synchronized and asynchronously growing cells of a V79 sub-line of the Chinese hamster were either partial-cell irradiation (λ, 254 nm) or laser-UV-microirradiated (λ, 257 nm). Post-incubation with caffeine (1–2 mM) often resulted in chromosome shattering, which was a rare event in the absence of th...

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Induction of chromosome damage by ultraviolet light and caffeine: Correlation of cytogenetic evaluation and flow karyotype

Cremer, Christoph; Cremer, Thomas; Gray, J. W.

Asynchrononously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated ( = 254 nm) with UV fluences up to 7.5 J/m2. After irradiation, cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected by mechanical shaking. Their chromosomes were ...

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Induction of beta-polymerase mRNA by DNA-damaging agents in Chinese hamster ovary cells.

Wilson, S H; Fornace, A J; Hollander, M C; Zmudzka, B

Only a few of the genes involved in DNA repair in mammalian cells have been isolated, and induction of a DNA repair gene in response to DNA damage has not yet been established. DNA polymerase beta (beta-polymerase) appears to have a synthetic role in DNA repair after certain types of DNA damage. Her...

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Induction of arsenite tolerance and thermotolerance by arsenite occur by different mechanisms.

Wang, Z; Rossman, T G; Hou, G

Both V79 and As/R28A cells (an arsenite-resistant Chinese hamster V79 cell variant) show increased resistance to toxic concentrations of arsenite after pretreatment with a nontoxic concentration. The induced tolerance can be completely inhibited by actinomycin D or cycloheximide. Pretreatment with a...

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Induction of a novel morphological response in Chinese hamster ovary cells by pertussis toxin.

Cowell, J L; Hewlett, E L; Myers, G A; Sauer, K T; Guerrant, R L

Exposing Chinese hamster ovary cells in culture to pertussis toxin resulted in a novel clustered growth pattern. The specificity of the response for pertussis toxin was shown by neutralization of the activity with specific anti-toxin antibody, heat lability (80 degrees C for 15 min), and absence of ...

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Induction of Thioguanine- and Ouabain-Resistant Mutants and Single-Strand Breaks in the DNA of Chinese Hamster Ovary Cells by 3H-Thymidine

Cleaver, James E.

Cultured Chinese hamster cells were labeled with 6-3H-thymidine or 5-methyl-3H-thymidine and allowed to accumulate damage from 3H decays for various periods of time while frozen. The frequencies of cells resistant to 6-thioguanine or ouabain and the amount of DNA damage (i.e., number of single-stran...

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Induction of Ouabain-resistant Mutation and Sister Chromatid Exchanges in Chinese Hamster Cells with Chemical Carcinogens Mediated by Human Pulmonary Macrophages

Trump, Benjamin F.; Hsu, Ih Chang; Yamaguchi, Maria; Harris, Curtis C.; Schafer, Paul W.

Pulmonary macrophages (PAM) metabolically activated benzo[a]pyrene [B(a)P] and its proximate carcinogenic metabolite, (±)trans 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (7,8-diol), to ultimate mutagens that were detected in cocultivated Chinese hamster V79 cells. Increases in the frequency of ouabainr...

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Induction of B2 RNA polymerase III transcription by heat shock: enrichment for heat shock induced sequences in rodent cells by hybridization subtraction.

Fornace, A J; Mitchell, J B

When hybridization subtraction was used to enrich for sequences induced by heat shock in Chinese hamster cells, B2 sequences were found to be one of the major sequences enriched. With cloned B2 probes, we found that the level of the short, 0.1 to 0.6 kb, polyadenylated RNA polymerase III transcripts...

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Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light

Chu, E. H. Y.; Chang, C. C.; Sun, N. C.

Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridin...

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Honokiol overcomes conventional drug resistance in human multiple myeloma by induction of caspase-dependent and -independent apoptosis

Roccaro, Aldo M.; Richardson, Paul; Hamasaki, Makoto; Le Gouill, Steven; Podar, Klaus; Chauhan, Dharminder; Kumar, Shaji

Honokiol (HNK) is an active component purified from magnolia, a plant used in traditional Chinese and Japanese medicine. Here we show that HNK significantly induces cytotoxicity in human multiple myeloma (MM) cell lines and tumor cells from patients with relapsed refractory MM. Neither coculture wit...

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Testis cancer cells are more sensitive than bladder and most other cancer cells to chemotherapeutic drugs both in the clinic and ipl vitro. In this study we show that they are also more sensitive than bladder cancer cells to heat. Since heat and drug sensitivity may be related to the ability of a ce...

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Gene induction and repression by salt treatment in roots of the salinity-sensitive Chinese Spring wheat and the salinity-tolerant Chinese Spring × Elytrigia elongata amphiploid

Gulick, Patrick; Dvořák, Jan

An artificial amphiploid from a cross between salinity-sensitive bread wheat cultivar Chinese Spring and highly tolerant Elytrigia elongata (Host) Nevski (= Agropyron elongatum Host) shows enhanced salinity tolerance relative to Chinese Spring. Poly(A)+ RNA was isolated from roots, expanding leaves,...

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Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions

Cederfur, Cecilia

Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since...

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Evidence for induction of oxidative stress caused by chronic exposure of Chinese residents to arsenic contained in drinking water.

Sun, Guifan; Pi, Jingbo; Kumagai, Yoshito; Yamauchi, Hiroshi; Yoshida, Takahiko; Shimojo, Nobuhiro; Aikawa, Hiroyuki

Exposure of experimental animals or cultured cells to arsenic induces oxidative stress, but, to date, no examination of this phenomenon in humans has been reported. In this study we conducted a cross-sectional study in Wuyuan, Inner Mongolia, China, to explore the relationship between chronic arseni...

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Effect on proliferation and apoptosis in RPM1-8226 human myeloma cells and regulation on steroid receptor coactivator-3 by deguelin

Li, R; Chen, Y; Shu, W-X

Objective: To investigate the effects of deguelin on the proliferation inhibition and apoptosis induction in RPMI-8226 cells in vitro, as well as the regulation of steroid receptor coactivator-3 (SRC-3) to explore the relationship between them. Methods: The effect of deguelin on the growth of RPMI-8...

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DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.

Tsuzuki, K; Haramaki, N; Sugiyama, M

The induction of cytotoxicity and DNA single-strand breaks by chromium(VI), cadmium(II), and mercury(II) were compared in H2O2-resistant Chinese hamster ovary (CHO(R)) cells and parental (CHO(P)) cells. Using a colony-forming assay, CHO(R) cells were found to be significantly more resistant than CHO...

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DNA adduct formation and mutation induction by nitropyrenes in Salmonella and Chinese hamster ovary cells: relationships with nitroreduction and acetylation.

Beland, F A; Heflich, R H; Djuric, Z; Fifer, E K

Nitrated pyrenes are environmental pollutants and potent mutagens in the Salmonella reversion assay. In this study reversion induction by 1-nitropyrene and 1,8-dinitropyrene in Salmonella typhimurium TA1538 and mutation induction by 1-nitropyrene in Chinese hamster ovary (CHO) cells were related to ...

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Cyclooxygenase-2 expression in esophageal cancer cells and induction by mitomycin C (in Chinese)

Zhuang, Z; Wang, LD; Tsao, GSW; Cheung, A; Feng, H; Yang, T; Qin, YR; Wang, CD; Chen, ZZ; Liu, B

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Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation.

Fornace, A J; Alamo, I; Schalch, H

Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript whic...

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Coordinate amplification of metallothionein I and II genes in cadmium-resistant Chinese hamster cells: implications for mechanisms regulating metallothionein gene expression.

Tesmer, J G; Munk, A C; Griffith, J K; Crawford, B D; Walters, R A; Griffith, B B; Enger, M D; Hanners, J L; Stallings, R L

We describe here the derivation, characterization, and use of clonal cadmium-resistant (Cdr) strains of the Chinese hamster cell line CHO which differ in their metallothionein (MT) induction capacity. By nondenaturing polyacrylamide gel electrophoresis, we showed that the stable Cdr phenotype is cor...

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Cell cycle synchronization: reversible induction of G2 synchrony in cultured rodent and human diploid fibroblasts.

Tobey, R A; Crissman, H A; Oishi, N

In accord with a set of prespecified principles of cell synchrony induction, a three-step procedure was developed to arrest cells reversibly in the G2 phase of the cell cycle. Cultures of Chinese hamster ovary (CHO) cells were presynchronized in early S phase by sequential treatment with isoleucine ...

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Bromodeoxyuridine-Induced Mutations in Synchronous Chinese Hamster Cells: Temporal Induction of 6-Thioguanine and Ouabain Resistance during DNA Replication

Burki, H. John; Aebersold, Paul M.

Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguani...

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Tam, Pui-yin, Edwin; 譚沛然

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Luo, Q.; Dai, D.C.; Yu, X.Y.; Qiu, Z.R.; Zhou, J.Y.; Yan, C.H.; Chen, Z.D.

Fluorescence interferometry is developed and applied to study ultrafast amplitude and phase dynamics for free-induction decay in powdered rare earth solids. The time-resolved phase dynamics of free-induction decay through-out the decaying process is accurately determined by using a novel dual-channe...

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