Sample records for 活体内 (in vivo)

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beta-Thalassemia in Chinese: use of in vivo RNA analysis and oligonucleotide hybridization in systematic characterization of molecular defects.

Kazazian, H H; Potter, M J; Cheng, T C; Antonarakis, S E; Orkin, S H; Sexton, J P; Li, A; Markham, A F; Giardina, P J

To perform a systematic analysis of beta-thalassemia genes among Chinese, we have determined the DNA haplotype in the beta-globin gene region of 37 Chinese beta-thalassemia chromosomes. Only four haplotypes were found. Blot hybridization analysis of erythroid RNA from patients homozygous for haploty...

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Turnip yellow mosaic virus RNA is aminoacylated in vivo in Chinese cabbage leaves

Joshi, S.; Haenni, A.L.; Chapeville, F.

Turnip yellow mosaic virus (TYMV) contains a tRNA-like structure as an integral part of its genome. This structure is located at the extreme 3' end of the viral RNA and is the acceptor of valine after 3'-terminal adenylation. It is known that in vitro (with bacterial, yeast, or plant systems) and in...

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Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo.

Liu, Q; Tong, Y; Sze, SCW; Liu, WK; Lam, L; Chu, ESM; Yow, CMN

ABSTRACT: BACKGROUND: Tian Xian Liquid (TXL) is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo. METHOD: HT-29 colon cancer cells were treated with gr...

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The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo.

Driscoll, J K; Sternlicht, H; Sternlicht, M L; Farr, G W; Willison, K; Yaffe, M B

A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled cells were analyzed by anion-ex...

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The specificity of UV-induced mutations at an endogenous locus in mammalian cells.

Drobetsky, E A; Glickman, B W; Grosovsky, A J

We have used a rapid in vivo recombinational method to clone and completely sequence 34 UV-induced mutants at the adenine phosphoribosyltransferase (APRT) locus of Chinese hamster ovary cells. Among the mutants recovered, 26 were single base substitutions including 17 G.C----A.T transitions and a si...

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TNFalpha-Polymorphismus bei Patienten mit Sepsis und Schilddrüsenkarzinomen

Rossbach, Christiane

Das Zytokin Tumornekrosefaktor a (TNFa) hat einen entscheidenden Anteil an der Entwicklung schwerer Komplikationen, wie septischer Schock und Multiorganversagen nach Entwicklung einer Sepsis. Eine Assoziation des TNF2-Allels mit einem erhöhten TNF a Spiegel und einer höheren Mortalitätsrate wurde i...

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Regulation of p21, MMP-1 and MDR-1 expression in Human colon carcinoma HT29 by Tian Xian Liquid, a Chinese Medicine Formula, in vitro and in vivo

Sze, CW; Wong, KL; Liu, WK; Ng, TB; Wong, JH; Yow, CMN; Chu, SME; Liu, Q; Hu, YM; Tsang, KW; Lee, WS; Tong, Y

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Pertussis toxin-catalyzed ADP-ribosylation of Gi-2 and Gi-3 in CHO cells is modulated by inhibitors of intracellular trafficking.

Xu, Y; Barbieri, J T

In previous studies, an in vitro ADP-ribosylation assay was developed to quantitatively evaluate the in vivo ADP-ribosylation of eukaryotic target proteins in intact Chinese hamster ovary (CHO) cells by pertussis toxin (PT). Immunoblot analysis identified the two PT-sensitive target proteins in CHO ...

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Opto-thermal moisture content and moisture depth profile measurements in organic materials

Xiao, P.; Guo, X.X.; Cui, Y.X.; Imhof, R.E.; Bicanic, D.D.

Opto-thermal transient emission radiometry (OTTER) is a infrared remote sensing technique, which has been successfully used in *in vivo* skin moisture content and skin moisture depth profiling measurements. In the present paper, we extend this moisture content measurement capability to analyze the m...

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On Microvascular Inflammation and Intestinal Leakage in Radiation Enteropathy

Röme, Andrada

Gastrointestinal tract damage is an insidious feature in patients undergoing radiotherapy. Microvascular inflammation, including leukocyte and platelet recruitment as well as epithelial barrier dysfunction, are considered to constitute key components in the pathophysiology of radiation-induced enter...

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New amber mutation in a beta-thalassemic gene with nonmeasurable levels of mutant messenger RNA in vivo.

Kazazian, H H; Atweh, G F; Zhu, X X; Brickner, H E; Forget, B G

We have identified a beta-thalassemia gene that carries a novel nonsense mutation in a Chinese patient. This mutation, a G to T substitution at the first position of codon 43, changes the glutamic acid coding triplet (GAG) to a terminator codon (TAG). Based on oligonucleotide hybridization studies o...

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Mismatch Repair by Efficient Nick-Directed, and Less Efficient Mismatch-Specific, Mechanisms in Homologous Recombination Intermediates in Chinese Hamster Ovary Cells

Nickoloff, J. A.; Miller, E. M.; Cho, J. W.; Hough, H. L.

Repair of single-base mismatches formed in recombination intermediates in vivo was investigated in Chinese hamster ovary cells. Extrachromosomal recombination was stimulated by double-strand breaks (DSBs) introduced into regions of shared homology in pairs of plasmid substrates heteroallelic at 11 p...

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Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.

Haynes, S R; Jelinek, W R

An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA [Haynes, S. R., Toomey, T. P., Leinwand, L. & Jelinek, W. R. (1981) Mol. Cell. Biol. 1, 573-583] serves as a template for cell-free transcription of discrete low molecular weight RNAs by RNA polymerase...

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Lack of correlation between expression of Epstein-Barr virus (EBV) latent membrane protein and bcl-2 oncoprotein in vivo.

Ho, F C; Tao, Q; Loke, S L; Srivastava, G

AIMS--To evaluate whether there is any correlation between the expression of Epstein-Barr virus (EBV) latent membrane protein (LMP) and oncoprotein bcl-2 in the lymph node biopsy specimens of a Chinese patient with EBV-related reactive lymphoproliferation who later developed T cell lymphoma after a ...

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Lack of correlation between expression of Epstein-Barr virus (EBV) latent membrane protein and bcl-2 oncoprotein in vivo

Tao, Q; Srivastava, G; Loke, SL; Ho, FCS

AIMS--To evaluate whether there is any correlation between the expression of Epstein-Barr virus (EBV) latent membrane protein (LMP) and oncoprotein bcl-2 in the lymph node biopsy specimens of a Chinese patient with EBV-related reactive lymphoproliferation who later developed T cell lymphoma after a ...

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Interactions between antiepileptic drugs and herbal medicines

Landmark, CJ; Patsalos, PN

As a therapeutic class, antiepileptic drugs (AEDs) have a high propensity to interact and many interactions with concomitant medications have been described. Increasingly, herbal medicines are often used by patients with epilepsy and the risk that these may interact with their AED medication is now ...

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Inhibition of metabolic cooperation by cigarette smoke condensate and its fractions in V-79 Chinese hamster lung fibroblasts.

Hartman, T G; Rosen, J D

This study was performed to determine the usefulness of the intercellular metabolic cooperation assay for analysis of a complex mixture and to compare the results obtained with previously conducted in vivo tumor promoter assays. One hundred 2R1 cigarettes were smoked according to Federal Trade Commi...

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In vitro and in vivo functional characterization of bovine vitamin K-dependent gamma-carboxylase expressed in Chinese hamster ovary cells.

Kuliopulos, A; Rehemtulla, A; Wasley, L C; Roth, D A; Walsh, C T; Kaufman, R J; Furie, B; Furie, B C

Coagulation factor IX is a serine protease for which high-level expression of biologically active protein in heterologous cells is limited due to inefficient proteolytic removal of the propeptide as well as vitamin K-dependent carboxylation of multiple amino-terminal glutamic acid residues. We have ...

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Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

Hu, YM; Wang, YT; Sze, SCW; Tsang, KW; Wong, HK; Liu, Q; Zhong, LD; Tong, Y

A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the ...

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Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions

Cederfur, Cecilia

Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since...

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Expression of vascular endothelial growth factor does not promote transformation but confers a growth advantage in vivo to Chinese hamster ovary cells.

Levinson, A D; Rowland, A; Ferrara, N; Burton, T; Winer, J; Siegel, M; Keller, G A; Phillips, H S; Terrell, T

Vascular endothelial growth factor (VEGF) is a mitogen with a specificity for endothelial cells in vitro and an angiogenic inducer in vivo. We tested the hypothesis that VEGF may confer on expressing cells a growth advantage in vivo. Dihydrofolatereductase--Chinese hamster ovary cells were transfect...

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Demonstration of non-functional beta-globin mRNA in homozygous beta (0) thalassemia.

Varmus, H E; Kan, Y W; Dozy, A M; Holland, J P

In two Chinese patients with homozygous beta(0)-thalassemia, messenger RNAs from peripheral blood reticulocytes and the bone marrow failed to direct beta-chain synthesis in vivo and in vitro in a cell-free system. Molecular hybridization showed that the beta cDNA annealed to the RNAs at almost the s...

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Comparison of Assay of Coliform Enterotoxins by Conventional Techniques Versus In Vivo Intestinal Perfusion

Short, Helen B.; Klipstein, Frederick A.; Wells, Joy G.; Guerrant, Richard L.; Engert, Richard F.

Thirty-six strains of coliform bacteria were tested for enterotoxigenicity both by conventional assays, including the Y-1 adrenal and Chinese hamster ovary cell assays for heat-labile toxin and the suckling mouse assay for heat-stable toxin, and by determining the ability of graded concentrations of...

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Clinical diagnosis of fissure caries with conventional and laser-induced fluorescence techniques

Chu, H. C.; Lo, M. C. E.; You, H. S. D.

We studied the in vivo validity of dentinal fissure caries diagnosis by visual examination, bitewing radiography, and use of a laser-induced fluorescence device (DIAGNOdent). A total of 144 and second molars with macroscopically intact occlusal surfaces in 41 Chinese young adults were examined visua...

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Bone turnover markers and prediction of bone loss in elderly women

Lenora, Janaka

Around 70,000 osteoporosis-related fractures occur in Sweden annually and approximately half of the women in western world will sustain a fragility fracture after the age of 50 years. Fracture preventive efforts require the identification of individuals who are at high risk. Biochemical markers of b...

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Accuracy of clinical estimates of intraocular pressure in Chinese eyes

Foster, PJ; Wong, JS; Wong, E; Chen, FG; Machin, D; Chew, PTK

Purposes To investigate the validity of clinical estimates of intraocular pressure (IOP) in Chinese people.Design: Prospective, cross-sectional, hospital-based in vivo study.Participants: Twenty-three ethnic Chinese adults (aged 35-82 years) undergoing routine phacoemulsification surgery were examin...

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A Mammalian Cell Mutant with a Temperature-Sensitive Leucyl-Transfer RNA Synthetase

Thompson, L. H.; Stanners, C. P.; Harkins, J. L.

A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely t...

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Chan, Ka-ho, John; 陳家豪

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