Sample records for 热能 (heat)

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Ubiquitin mRNA is a major stress-induced transcript in mammalian cells.

Lamoreaux, E; Fornace, A J; Hollander, M C; Alamo, I

Ubiquitin mRNA was found to be an abundant transcript which was induced by heat shock (HS), and certain other stresses in mammalian cells. In Chinese hamster cells, the 2 major ubiquitin transcripts of 2.6 kb and 1.7 kb were induced coordinately, while a minor ubiquitin transcript of 0.8 kb was not ...

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TNFalpha-Polymorphismus bei Patienten mit Sepsis und Schilddrüsenkarzinomen

Rossbach, Christiane

Das Zytokin Tumornekrosefaktor a (TNFa) hat einen entscheidenden Anteil an der Entwicklung schwerer Komplikationen, wie septischer Schock und Multiorganversagen nach Entwicklung einer Sepsis. Eine Assoziation des TNF2-Allels mit einem erhöhten TNF a Spiegel und einer höheren Mortalitätsrate wurde i...

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Synthesis and behaviour of small RNA species of CHO cells submitted to a heat chick.

Zalta, J P; Bouche, G; Amalric, F; Caizergues-Ferrer, M; Banville, D; Simard, R

Incubation of Chinese hamster ovary (CHO) cells for 1 hour at 43 degrees C results in an inhibition of high molecular weight RNA synthesis while most of the ow molecular weight RNAs are still synthesized. In cells returned to 37 degrees C, the transcription of high molecular weight RNA is reinitiate...

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PIV and CFD measurements of internal velocity in a forming drop in a liquid-liquid system

Timgren, Anna; Trägårdh, Gun; Trägårdh, Christian

A PIV method has been used to determine the internal motion in an oil drop during formation and to validate a numerical simulation of the drop formation process. The PIV system included a microscope attached to the camera, which gave a focal depth of 50 µm and a possibility to measure the velocity i...

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Model predictive control of a powder coating curing process: an application of the MPC@CB© software

Abid, Kamel; Dufour, Pascal; Bombard, Isabelle; Laurent, Pierre

This paper deals with the control of a powder coating radiative curing process by infrared flow. It is based on a unidirectional dynamic modelling where both heat transfert and cure phenomena are accounted for within the thicknessl. The control problem is the constrained optimization of the curing c...

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Lycium barbarum polysaccharides: Protective effects against heat-induced damage of rat testes and H2O2-induced DNA damage in mouse testicular cells and beneficial effect on sexual behavior and reproductive function of hemicastrated rats

Luo, Q; Li, ZN; Huang, XL; Yan, J; Zhang, SH; Cai, Y

Lycium barbarum, a famous Chinese medicinal herb, has a long history of use as a traditional remedy for male infertility. Polysaccharides are the most important functional constituent in L. barbarum fruits. We systematically investigated the effect of L. barbarum polysaccharides (LBP) on rat testis ...

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Kinetic Modeling of Isothermal or Non-isothermal Adsorption in a Pellet: Application to Adsorption Heat Pumps

Montastruc, Ludovic; Floquet, Pascal; Mayer, Volker; Nikov, Iordan; Domenech, Serge

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Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation.

Sciandra, J J; Hughes, C S; Subjeck, J R

In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environmen...

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Induction of B2 RNA polymerase III transcription by heat shock: enrichment for heat shock induced sequences in rodent cells by hybridization subtraction.

Fornace, A J; Mitchell, J B

When hybridization subtraction was used to enrich for sequences induced by heat shock in Chinese hamster cells, B2 sequences were found to be one of the major sequences enriched. With cloned B2 probes, we found that the level of the short, 0.1 to 0.6 kb, polyadenylated RNA polymerase III transcripts...

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Inducible overproduction of the mouse c-myc protein in mammalian cells.

Wurm, F M; Kingston, R E; Gwinn, K A

We have made Chinese hamster ovary (CHO) cell lines that contain up to 2000 copies of the coding region of the mouse c-myc gene fused to the promoter of the Drosophila gene (hsp70) encoding a Mr 70,000 heat shock protein. Incubation of these cells at 43 degrees C results in an estimated 100-fold ind...

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Identification of a protein altered in mutants resistant to microtubule inhibitors as a member of the major heat shock protein (hsp70) family.

Gupta, R S; Ahmad, S; Venner, T J; Ahuja, R

A major cellular protein (P2; approximately 70 kilodaltons) which is altered in Chinese hamster ovary (CHO) cell mutants resistant to the microtubule inhibitors colchicine and podophyllotoxin has been shown to correspond to the constitutive form of the 70-kilodalton heat shock protein (hsc70). The i...

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Identification of a cytotoxin produced by Legionella pneumophila.

Friedman, R L; Miller, R D; Iglewski, B H

Culture filtrates of Legionella pneumophila were cytotoxic for Chinese hamster ovary cells. The cytotoxin was found to be methanol soluble, heat stable, and stable from pH 5 through 8.

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Helicobacter pylori Heat Shock Protein A: Serologic Responses and Genetic Diversity

Blaser, Martin J.; Kansau, Imad; Ng, Enders K. W.; Pérez-Pérez, Guillermo I.; Thompson, Stuart A.; van der Ende, Arie

Helicobacter pylori synthesizes an unusual GroES homolog, heat shock protein A (HspA). The present study was aimed at an assessment of the serological response to HspA in a group of Chinese patients with defined gastroduodenal pathologies and determination of whether diversity is present in the nucl...

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Heat-resistant variants of Chinese hamster fibroblasts altered in expression of heat shock protein.

Laszlo, A; Li, G C

Heat-resistant variants of the Chinese hamster HA-1 line have been isolated after repeated heat treatments. The heat-resistant phenotype has been stable for over 70 passages. One of the members of the 70-kDa heat shock protein family was found to be synthesized at greater levels in the heat-resistan...

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Testis cancer cells are more sensitive than bladder and most other cancer cells to chemotherapeutic drugs both in the clinic and ipl vitro. In this study we show that they are also more sensitive than bladder cancer cells to heat. Since heat and drug sensitivity may be related to the ability of a ce...

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Escherichia coli heat-labile enterotoxin: comparison of antitoxin assays and serum antitoxin levels.

Wachsmuth, I K; Ryder, R W; Wells, J G

The mouse adrenal tumor cells (Y-1 strain) and the Chinese hamster ovary cells, two routinely used tissue culture assays for Escherichia coli heat-labile enterotoxin (LT), were used to detect serum antitoxin responses in culture-positive patients from several well-defined sources. There was no corre...

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Escherichia coli heat-labile enterotoxin: DNA-directed in vitro synthesis and structure.

Högenauer, G; Dorner, F; Nahler, G; Hughes, C

Escherichia coli heat-labile enterotoxin was synthesized in a cell-free system directed by DNA of the plasmid P307. Synthesis of the toxin, assayed by the elongation induced in Chinese hamster ovary cells, was strongly stimulated by cyclic AMP and occurred at physiological levels of Mg2+ only when t...

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Enterotoxigenic Bacteria in Food and Water from an Ethiopian Community

Jiwa, Sadruddin F. H.; Wadström, Torkel; Krovacek, Karel

Food and water samples from an Ethiopian community were screened for the presence of enterotoxin-producing bacteria. Using the Chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (LT) enterotoxin. These LT-producing isolates comprised 33 of 177 (18.6%) strains from 24 o...

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Effects of heat shock on gene expression and subcellular protein distribution in Chinese hamster ovary cells.

Zalta, J P; Bouche, G; Caizergues-Ferrer, M; Amalric, F

Incubation of Chinese Hamster Ovary (CHO) cells for one hour at 43 degrees C results in several obvious changes in protein distribution and protein synthesis. One major protein of the cytoplasm (molecular weight 45,000 daltions), also present as a minor component in the nucleus, rapidly disappeared ...

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Effect of overexpression of the small heat shock protein HSP27 on the heat and drug sensitivities of human testis tumor cells

Richards, EH; Hickey, E; Weber, L; Masters, JRW

In contrast to most metastatic cancers, testicular germ cell tumors are cured in more than 80% of patients using cisplatin-based combination chemotherapy. Testis tumor cells in vitro retain their sensitivity to chemotherapeutic drugs, radiation, and other stresses, such as heat shock. saving shown t...

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Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts.

Li, G C; Werb, Z

Synthesis of a family of proteins called "heat shock" proteins is induced or enhanced in cells in response to various environmental stresses, suggesting that these proteins may perform functions essential to cell survival. Because a brief, nonlethal heat treatment can dramatically induce a transient...

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Comparison of effects of nicked and unnicked Escherichia coli heat-labile enterotoxin on Chinese hamster ovary cells.

Tsuji, T; Miwatani, T; Honda, T

The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change (elongation) of CHO cells from round to spindle-shaped cells faster than did unnicked LT. The times fo...

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Comparison of Assay of Coliform Enterotoxins by Conventional Techniques Versus In Vivo Intestinal Perfusion

Short, Helen B.; Klipstein, Frederick A.; Wells, Joy G.; Guerrant, Richard L.; Engert, Richard F.

Thirty-six strains of coliform bacteria were tested for enterotoxigenicity both by conventional assays, including the Y-1 adrenal and Chinese hamster ovary cell assays for heat-labile toxin and the suckling mouse assay for heat-stable toxin, and by determining the ability of graded concentrations of...

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CFD modelling of drop formation in a liquid-liquid system

Timgren, Anna; Trägårdh, Gun; Trägårdh, Christian

The formation of an oil drop from a single capillary in a continuous phase flowing perpendicular to the capillary opening has been studied numerically. The shear stress at the interface of the forming drop, the angular velocity inside the drop and the pressure field around the drop have been determi...

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A multiple center, randomized controlled, double-blinded and double-dummy trial of Shuangjie Capsule (Piece) in the treatment of the acute upper respiratory tract infection (the syndrome of heat attacking the lung and weifen)

Wang, L; Zhang, R-M; Zhao, Y-L; Feng, G-X; Pan, D-J; Huang, X-Y; Mao, B; Wang, G; Chang, J; Zhang, Y; Wan, M-H; Guo, J; Chen, Y

Objective: To assess the effectiveness and safety of Shuangjie Capsule (Piece) in the treatment of acute upper respiratory tract infection. Methods: The multiple center, double-blinded, double-dummy and randomized controlled method was adopted to observe 770 patients who were divided into the treatm...

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A global overview of drought and heat-induced tree mortality reveals emerging climate change risks for forests

Allen, C.D.; Macalady, A.K.; Chenchouni, H.; Bachelet, D.; McDowell, N.; Vennetier, M.; Kitzberger, T.; Rigling, A.

Greenhouse gas emissions have significantly altered global climate, and will continue to do so in the future. Increases in the frequency, duration, and/or severity of drought and heat stress associated with climate change could fundamentally alter the composition, structure, and biogeography of fore...

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