Sample records for 基因 (genes)
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36

α0 Thalassaemia as a result of a novel 11.1 kb deletion eliminating both of the duplicated α globin genes

Liao, C; Jia, S-Q; Mo, Q-H; Li, J; Li, L-Y; Xu, X-M

Aims: To characterise a novel 11.1 kb deletion that eliminated both of the duplicated α globin genes, giving rise to a typical α0 thalassaemia phenotype in four carriers from a Chinese family.

DRIVER (Chinese)

39

beta-Thalassemia in Chinese: use of in vivo RNA analysis and oligonucleotide hybridization in systematic characterization of molecular defects.

Kazazian, H H; Potter, M J; Cheng, T C; Antonarakis, S E; Orkin, S H; Sexton, J P; Li, A; Markham, A F; Giardina, P J

To perform a systematic analysis of beta-thalassemia genes among Chinese, we have determined the DNA haplotype in the beta-globin gene region of 37 Chinese beta-thalassemia chromosomes. Only four haplotypes were found. Blot hybridization analysis of erythroid RNA from patients homozygous for haploty...

DRIVER (Chinese)

40

Tuberculosis candidate genes in Chinese

Tso, HW; Ip, EWK; Chong, WP; Tam, CM; Chiang, AKS

DRIVER (Chinese)

41

Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells.

Thielmann, H W; Kaina, B; Backendorf, C; Van Zeeland, A A; Van de Putte, P

Chinese hamster ovary cells were transfected by human DNA ligated to the bacterial gpt (xanthine-guanine-phosphoribosyltransferase) gene which was used either in its native form or after partial inactivation with methylnitrosourea. The gpt+ transfectants were screened for resistance to high doses of...

DRIVER (Chinese)

42

Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

Cho, Seungho; Muehlbauer, Gary J.; Garvin, David F.

Wheat–barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese Spring–Betzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe array. Of...

DRIVER (Chinese)

47

The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes.

Barker, P E; Czosnek, H; Shani, M; Nudel, U; Pravtcheva, D D; Ruddle, F H; Yaffe, D

The chromosomal distribution of murine genes expressed during differentiation of skeletal muscle cells was determined by Southern blot analysis of DNA from mouse-Chinese hamster hybrid cell lines containing incomplete subsets of mouse chromosomes. All detectable myosin heavy chain genes are located ...

DRIVER (Chinese)

48

The gene and the pseudogene for mouse p53 cellular tumor antigen are located on different chromosomes.

Zakut-Houri, R; Czosnek, H H; Givol, D; Bienz, B; Pravtcheva, D D; Ruddle, F H; Oren, M

The chromosomal assignments of the two genes encoding the murine p53 cellular tumor antigen were determined by using a panel of mouse-Chinese hamster somatic cell hybrid clones and a mouse p53-specific cDNA clone. One gene, probably the functional member of the family, was found to be on chromosome ...

DRIVER (Chinese)

49

The chromosomal location of mouse interferon alpha genes.

Boll, W; Lovett, M; Yee, D; Cox, D R; Weissmann, C; Epstein, C J; Epstein, L B

The chromosomal location of mouse leukocyte-interferon (IFN-alpha) genes was determined by Southern blot analysis of DNA from a panel of Chinese hamster x mouse somatic cell hybrids using a mouse IFN-alpha cDNA as a hybridization probe. All resolvable mouse genes are located on mouse chromosome 4. I...

DRIVER (Chinese)

54

Streptogramin- and tetracycline-responsive dual regulated expression of p27Kip1 sense and antisense enables positive and negative growth control of Chinese hamster ovary cells

Rimann, Markus; Fux, Cornelia; Schlatter, Stefan; Moser, Samuel; Bailey, James E.; Fussenegger, Martin

We constructed a dual regulated expression vector cassette (pDuoRex) whereby two heterologous genes can be independently regulated via streptogramin- and tetracycline-responsive promoters. Two different constructs containing growth-promoting and growth-inhibiting genes were stably transfected in rec...

DRIVER (Chinese)

55

Strand specificity for UV-induced DNA repair and mutations in the Chinese hamster HPRT gene.

van Zeeland, A A; van Hoffen, A; Vrieling, H; van Rooyen, M L; Venema, J; Menichini, P; Mullenders, L H; Zdzienicka, M Z

DNA excision repair modulates the mutagenic effect of many genotoxic agents. The recently observed strand specificity for removal of UV-induced cyclobutane dimers from actively transcribed genes in mammalian cells could influence the nature and distribution of mutations in a particular gene. To inve...

DRIVER (Chinese)

57

Specificity of the cAMP-induced gene exposure reaction in CHO cells.

Ashall, F; Puck, T T; Sullivan, N

Previous studies demonstrated that in the transformed CHO (Chinese hamster ovary) cell a substantial part of the genome behaves as though its genes are sequestered from effective contact with soluble constituents of the intracellular fluid. The reverse transformation reaction, initiated by cAMP deri...

DRIVER (Chinese)

58

Simultaneous expression of two P-glycoprotein genes in drug-sensitive Chinese hamster ovary cells.

Gerlach, J H; Endicott, J A; Sarangi, F; Juranka, P F; Deuchars, K L; Ling, V

Overexpression of P-glycoprotein is characteristic of multidrug-resistant cells. We analyzed four P-glycoprotein transcripts that are simultaneously expressed in a drug-sensitive Chinese hamster ovary cell line. We concluded that these transcripts are encoded by two distinct members of a P-glycoprot...

DRIVER (Chinese)

59

Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5.

Dana, S; Wasmuth, J J

Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (en...

DRIVER (Chinese)

60

Segregation and rearrangement of coamplified genes in different lineages of mutant cells that overproduce adenylate deaminase.

de Saint-Vincent, B R; Debatisse, M; Petit-Koskas, E; Hyrien, O; Buttin, G

Four genes encoding proteins designated as W, X, Y1, and Y2 were found previously to be amplified at different levels in a Chinese hamster fibroblast mutant line selected for overproduction of adenylate deaminase. To gain information on the molecular mechanisms responsible, we studied the levels of ...

DRIVER (Chinese)

61

Repair of N-methylpurines in specific DNA sequences in Chinese hamster ovary cells: absence of strand specificity in the dihydrofolate reductase gene.

Scicchitano, D A; Hanawalt, P C

We have developed a quantitative method for examining the removal of N-methylpurines from specific genes to investigate their possible differential repair throughout the genome. Chinese hamster ovary cells were exposed to dimethyl sulfate, and the isolated DNA was treated with an appropriate restric...

DRIVER (Chinese)

62

Receptor-mediated endocytosis of low density lipoprotein: somatic cell mutants define multiple genes required for expression of surface-receptor activity.

Kingsley, D M; Krieger, M

We have used cell fusion and mutant reversion analysis to study a collection of Chinese hamster ovary (CHO) cell mutants that are unable to bind and internalize low density lipoprotein (LDL). Pairwise cell fusions show that these LDL receptor-deficient mutants fall into three recessive complementati...

DRIVER (Chinese)

63

Random cloning of genes from mouse chromosome 17.

Kasahara, M; Klein, J; Figueroa, F

We describe a method for isolating cosmid clones randomly from mouse chromosome 17. A cosmid library was constructed from the mouse-Chinese hamster cell line R4 4-1 that contains a limited amount of mouse DNA (chromosomes 17 and 18 and some other unidentified material) on a Chinese hamster backgroun...

DRIVER (Chinese)

65

Purification and Characterization of Novel Toxin Produced by Vibrio cholerae O1

Nair, G. B.; Walia, Kamini; Singh, Harminder; Ghosh, Sujata; Ghosh, Amit; Ganguly, Nirmal Kumar; Sahni, Girish; Vohra, Harpreet

Vibrio cholerae WO7 (serogroup O1) isolated from patients with diarrhea produces an extracellular toxin despite the absence of ctx, zot, and ace genes from its genome. The toxin elongates Chinese hamster ovary cells, produces fluid accumulation in ligated rabbit ileal loops, and agglutinates freshly...

DRIVER (Chinese)

66

Protein-DNA interactions at the major and minor promoters of the divergently transcribed dhfr and rep3 genes during the Chinese hamster ovary cell cycle.

Heintz, N H; Wells, J; Illenye, S; Held, P

In mammals, two TATA-less bidirectional promoters regulate expression of the divergently transcribed dihydrofolate reductase (dhfr) and rep3 genes. In CHOC 400 cells, dhfr mRNA levels increase about fourfold during the G1-to-S phase transition of the cell cycle, whereas the levels of rep3 transcript...

DRIVER (Chinese)

67

Presence and transcription of intracisternal A-particle-related sequences in CHO cells.

Williams, S R; Anderson, K P; Low, M A; Lie, Y S; Fennie, E H; Nguyen, T P; Wurm, F M

We have characterized sequences expressed in Chinese hamster ovary (CHO) cells which are related to the intracisternal A-particle (IAP) genes of mice and Syrian hamsters. Several cDNA clones homologous to Syrian hamster IAP probes have been isolated and used to evaluate the abundance and expression ...

DRIVER (Chinese)

68

Population differences in SLE susceptibility genes: STAT4 and BLK, but not PXK, are associated with systemic lupus erythematosus in Hong Kong Chinese

Yang, W; Ng, P; Zhao, M; Hirankarn, N; Lau, CS; Mok, CC; Chan, TM; Wong, RWS; Lee, KW; Mok, MY; Wong, SN; Avihingsanon, Y; Lee, TL

In this study, we compared the association of several newly discovered susceptibility genes for systemic lupus erythematosus (SLE) between populations of European origin and two Asian populations. Using 910 SLE patients and 1440 healthy controls from Chinese living in Hong Kong, and 278 SLE patients...

DRIVER (Chinese)

69

Population differences in SLE susceptibility genes STAT4 and BLK, but not PXK, are associated with systemic lups erthematosus in Hong Kong Chinese [Epub 19 Feb 2009]

Yang, W; Ng, P; Zhao, M; Hirankarn, N; Lau, WCS; Mok, CC; Chan, DTM; Wong, RWS; Lee, KW; Mok, TMY; Wong, SN; Avihingsanon, Y

DRIVER (Chinese)

70

Population differences in SLE susceptibility genes - STAT4 and BLK, but not PXK, are associated with Systemic Lupus Erythematosus in Hong Kong Chinese

Yang, W; Ng, P; Zhao, M; Hirankarn, N; Lau, CS; Mok, CC; Chan, TM; Wong, RMY; Lee, KW; Mok, MY; Wong, SN; Avihingsanon, Y; Lee, TL

DRIVER (Chinese)

71

Polymorphisms of CYP and GST genes, passive smoke and lung cancer risk in Hong Kong Chinese

Chan, MMW; Lam, WK; Mak, JCW; Ho, SP; Chan, HW; Tsang, KWT; Ip, MSM; Ho, JCM; Wong, MP; Tan-Un, K

DRIVER (Chinese)

73

Polymerase chain reaction based C4AQ0 and C4BQ0 genotyping: association with systemic lupus erythematosus in southwest Han Chinese

Yao, Y; Man, X; Li, X; Luo, H; Mao, C; Zhang, Y

Methods: C4 null genes were determined by a polymerase chain reaction (PCR) procedure with sequence specific primers (PCR-SSP). The 2 bp insertion in exon 29, which was previously identified in non-Chinese populations and caused defective C4A genes, was directly typed by sequencing the whole exon 29...

DRIVER (Chinese)

74

Pattern of methylation of two genes coding for housekeeping functions.

Cedar, H; Stein, R; Razin, A; Sciaky-Gallili, N

The distribution of sites that can be methylated was analyzed in the Chinese hamster adenine phosphoribosyl-transferase (aprt) gene and the patterns of methylation of this gene and the mouse dihydrofolate reductase (dhfr) gene were studied by using CpG restriction enzymes. Both genes were found to b...

DRIVER (Chinese)

75

PDCD1 genes may protect against extraocular manifestations in Chinese Han patients with Vogt-Koyanagi-Harada syndrome

Meng, Q.L.; Liu, X.L.; Yang, P.Z.; Hou, S.P.; Du, L.P.; Zhou, H.Y.; Kijlstra, A.

Purpose: To analyze the potential association of programmed cell death 1 (PDCD1) with Vogt-Koyanagi-Harada (VKH) syndrome in a Chinese Han population. Methods: Three single nucleotide polymorphism (SNPs), PD-1.3G/A, PD-1.5C/T, and PD-1.6G/A, were genotyped in 247 VKH patients and 289 age-, sex-, and...

DRIVER (Chinese)

76

Overexpression and amplification of five genes in a multidrug-resistant Chinese hamster ovary cell line.

Borst, P; Van der Bliek, A M; Ling, V; Van der Velde-Koerts, T

Multidrug-resistant cells are cross-resistant to a wide range of unrelated drugs, many of which are used in cancer chemotherapy. We constructed a cDNA library from RNA of the multidrug-resistant Chinese hamster ovary cell line CHRC5. By differential screening we isolated cDNAs derived from mRNAs tha...

DRIVER (Chinese)

77

Organization of the alpha-globin genes in the Chinese alpha-thalassemia syndromes.

Kan, Y W; Embury, S H; Dozy, A M; Lebo, R V

The alpha-thalassemia syndromes are a group of inherited anemias, the clinical severity of which has been shown to increase with the number of alpha-globin structural genes deleted. Employing restriction endonuclease gene mapping, we defined the organization of the alpha-globin genes in cellular DNA...

DRIVER (Chinese)

78

Mutational analysis of BRCA1 and BRCA2 genes in Chinese ovarian cancer identifies 6 novel germline mutations

Khoo, US; Ngan, HYS; Cheung, ANY; Chan, YK; Lu, J; Chan, VWY; Lau, S; Andrulis, IL; Ozcelik, H

DRIVER (Chinese)

82

Microcell-mediated cotransfer of genes specifying methotrexate resistance, emetine sensitivity, and chromate sensitivity with Chinese hamster chromosome 2.

Worton, R; Flintoff, W; Duff, C

Many selectable mutants of somatic Chinese hamster cells have been described, but very few of the mutations have been mapped to specific chromosomes. We have utilized the microcell-mediated gene transfer technique to establish the location of three selectable genetic markers on chromosome 2 of Chine...

DRIVER (Chinese)

85

Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.

Dijkwel, P A; Hamlin, J L

Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (a...

DRIVER (Chinese)

86

Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids.

Hastie, N D; Bennett, K L; Barth, R K; Lalley, P A

We have mapped the multiple (15-25) genes coding for the hormonally regulated major urinary proteins (MUPs) of the mouse by using a cloned cDNA probe. By Southern blot analysis of DNA from Chinese hamster-mouse somatic cell hybrids, all of the MUP genes were found to be on chromosome 4. Different in...

DRIVER (Chinese)

91

Lack of structural rearrangement in c-kit and stem cell factor genes in Hong Kong Chinese patients with myelodysplastic syndromes or acute myeloid leukaemia

Chui, CH; Leung, PHM; Lau, FY; Wan, TSK; Chan, LC; Cheng, G

Stem cell factor is a haemopoietic growth factor that interacts with the c-kit--encoded transmembrane tyrosine kinase receptor during signal transduction in haemopoietic progenitor stem cells. We have screened 127 Chinese patients with myelodysplastic syndromes or acute myeloid leukaemia for structu...

DRIVER (Chinese)

92

Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.

Chang, W; Bair, C H; Vasilevskaya, I A; Chung, C S

The Chinese hamster ovary (CHO) cell line is nonpermissive for vaccinia virus, and translation of viral intermediate genes was reported to be blocked (A. Ramsey-Ewing and B. Moss, Virology 206:984-993, 1995). However, cells are readily killed by vaccinia virus. A vaccinia virus-resistant CHO mutant,...

DRIVER (Chinese)

95

Inhibition of protein synthesis stimulates the transcription of human beta-interferon genes in Chinese hamster ovary cells.

Trahey, M; Ringold, G M; Vannice, J L; Dieckmann, B; McCormick, F

Using Chinese hamster ovary (CHO) cells transfected with a plasmid carrying the human beta-interferon gene, we find that inhibitors of protein synthesis, in the absence of any other inducer, stimulate the production of interferon RNA; this effect is maintained in cells in which the plasmid sequences...

DRIVER (Chinese)

96

Induction of beta-polymerase mRNA by DNA-damaging agents in Chinese hamster ovary cells.

Wilson, S H; Fornace, A J; Hollander, M C; Zmudzka, B

Only a few of the genes involved in DNA repair in mammalian cells have been isolated, and induction of a DNA repair gene in response to DNA damage has not yet been established. DNA polymerase beta (beta-polymerase) appears to have a synthetic role in DNA repair after certain types of DNA damage. Her...

DRIVER (Chinese)

97

Inducible expression of amplified human beta interferon genes in CHO cells.

Dieckmann, B; McCormick, F; Innis, M; Trahey, M; Ringold, G

Plasmid DNA containing the human beta-interferon (IFN-beta) gene and mouse dihydrofolate reductase cDNA was transfected into dihydrofolate reductase-negative Chinese hamster ovary cells. Dihydrofolate reductase-positive transformants were obtained, and cells containing amplified copies of mouse dihy...

DRIVER (Chinese)

99

Identification of osteoporosis susceptibility genes in Hong Kong Chinese

Huang, Q; Li, HY; Cheung, CL; Kung, AWC

International Conference on Osteoporosis and Bone Research 2008, 22-25 October 2008, Beijing, China

DRIVER (Chinese)

104

Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.

Caskey, C T; Konecki, D S; Fuscoe, J C; Brennand, J; Chinault, A C

Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDN...

DRIVER (Chinese)

106

Human Acyl-Coenzyme A:Cholesterol Acyltransferase Expressed in Chinese Hamster Ovary Cells: Membrane Topology and Active Site Location

Chang, Ta-Yuan; Lin, Song; Chang, Catherine C.Y.; Lu, Xiaohui

Acyl-CoA:cholesterol acyltransferase (ACAT) is a membrane-bound enzyme that produces cholesteryl esters intracellularly. Two ACAT genes (ACAT1 and ACAT2) have been identified. The expression of ACAT1 is ubiquitous, whereas that of ACAT2 is tissue restricted. Previous research indicates that ACAT1 ma...

DRIVER (Chinese)

107

Heritability of the iridotrabecular angle width measured by optical coherence tomography in Chinese children: The Guangzhou twin eye study

He, MG; Ge, J; Wang, DD; Zhang, J; Hewitt, AW; Hur, YM; Mackey, DA; Foster, PJ

PURPOSE. To estimate the heritability of the iridotrabecular angle width measured by anterior segment optical coherence tomography (ASOCT) in a classic twin study.METHODS. Twins aged 8 to 16 years were identified from the Guangzhou Twin Registry. ASOCT was used to obtain one horizontal scan, and the...

DRIVER (Chinese)

108

Heritability of anterior chamber depth as an intermediate phenotype of angle-closure in Chinese: The Guangzhou twin eye study

He, MG; Wang, DD; Zheng, YF; Zhang, J; Yin, QX; Huang, WY; Mackey, DA; Foster, PJ

PURPOSE. To assess the heritability of anterior chamber depth (ACD) and relative anterior chamber depth (ACD/axial length, rACD) in Chinese in a classic twin study.METHODS. Twins aged 7 to 15 years living in two local districts were recruited from the Guangzhou Twin Registry. Anterior chamber depth ...

DRIVER (Chinese)

109

Hemoglobin synthesis in somatic cell hybrids: coexpression of mouse with human or chinese hamster globin genes in interspecific somatic cell hybrids of mouse erythroleukemia cells.

Nienhuis; Deisseroth, A; Anderson, W F; Barker, J

Somatic cell hybrids were derived by fusion of mouse erythroleukemia cells with fractionated human marrow enriched in erythroblasts, or with chinese hamster fetal liver erythroid cells. Such interspecific hybrid cells, when isolated in suspension culture, had retained nearly all the mouse chromosome...

DRIVER (Chinese)

112

Genetics and clinical features of Hemoglobin H disease in Chinese patients

Chen, FE; Ooi, CGC; Ha, SY; Cheung, BMY; Todd, D; Liang, RHS; Chan, TK; Chan, VNY

Background Normally, one pair of each of the two -globin genes, 1 and 2, resides on each copy of chromosome 16. In hemoglobin H disease, three of these four -globin genes are affected by a deletion, a mutation, or both. We studied the -globin gene abnormalities and the clinical and hematologic featu...

DRIVER (Chinese)

113

Genetic and Clinical Features of Hemoglobin H Disease in Chinese Patients

Chen, FE; Ooi, CGC; Ha, SY; Cheung, BMY; Todd, D; Liang, RHS; Chan, TK; Chan, V

Background Normally, one pair of each of the two -globin genes, 1 and 2, resides on each copy of chromosome 16. In hemoglobin H disease, three of these four -globin genes are affected by a deletion, a mutation, or both. We studied the -globin gene abnormalities and the clinical and hematologic featu...

DRIVER (Chinese)

114

Genetic analysis of tumorigenesis: a conserved region in the human and Chinese hamster genomes contains genetically identified tumor-suppressor genes.

Stenman, G; Sager, R

Regional chromosome homologies were found in a comparison of human 11p with Chinese hamster 3p. By use of probes that recognize six genes of human 11p (INS, CAT, HBBC, CALC, PTH, and HRAS), the corresponding genes were localized by in situ hybridization on Chinese hamster chromosome 3. INS and CAT w...

DRIVER (Chinese)

115

Genetic Classification of “Rickettsia heilongjiangii” and “Rickettsia hulinii,” Two Chinese Spotted Fever Group Rickettsiae

Fournier, P. E.; Zhang, J. Z.; Wu, Y. M.; Fan, M. Y.; Roux, V.; Raoult, D.

To determine the phylogenetic position of two new rickettsial strains isolated from ticks in China, 16S ribosomal DNA, gltA, and ompA (apart from the tandem repeat units) genes were amplified by PCR and sequenced. The phylogenetic relationships between these strains and other rickettsiae were inferr...

DRIVER (Chinese)

116

Genes controlling receptors for ecotropic and xenotropic type C virus in Mus cervicolor and Mus musculus.

Marshall, T H; Rapp, U R

Gene loci controlling cell surface receptors for murine leukemia virus were studied by using murine X Chinese hamster hybrid cells. Hybrids which exclusively segregate murine chromosomes were made by fusing Mus cervicolor and Mus musculus lymphocytes to hamster fibroblasts. Sensitivity to Moloney mu...

DRIVER (Chinese)

118

Expression of several amplified genes in an adenylate-deaminase overproducing variant of Chinese hamster fibroblasts.

Debatisse, M; Buttin, G; de Saint Vincent, B R

Unstable variants with increasing amounts of adenylate-deaminase (AMPD) have been stepwise recovered from Chinese hamster fibroblasts plated in selective medium containing increasing coformycin concentrations; several polypeptides accumulate in the variants in parallel to AMPD: they are no longer de...

DRIVER (Chinese)

121

Expression of biologically active bovine luteinizing hormone in Chinese hamster ovary cells.

Nett, T M; Kaetzel, D M; Wondisford, F; Browne, J K; Thomason, A R; Nilson, J H

Biologically active bovine luteinizing hormone (LH) has been obtained through expression of the alpha- and LH beta-subunit genes in stably transformed clones of DUXB11, a Chinese hamster ovary cell line deficient in dihydrofolate reductase (DHFR). Expression of alpha-and LH beta-subunit mRNAs of the...

DRIVER (Chinese)

122

Expression cloning of LDLB, a gene essential for normal Golgi function and assembly of the ldlCp complex

Bickel, Perry E.; Chatterton, Jon E.; Schwartz, John J.; Hirsch, David; Rosenberg, Robert D.; Lodish, Harvey F.

The Chinese hamster ovary (CHO) cell mutants ldlC and ldlB, which exhibit almost identical phenotypes, define two genes required for multiple steps in the normal medial and trans Golgi-associated processing of glycoconjugates. The LDLC gene encodes ldlCp, an ≈80-kDa protein, which in wild-type, but ...

DRIVER (Chinese)

123

Evidence for plasmid- and chromosome-borne multiple nif genes in Rhizobium fredii.

Barbour, W M; Elkan, G H; Mathis, J N

Rhizobium fredii is a fast-growing rhizobium isolated from the primitive Chinese soybean cultivar Peking and from the wild soybean Glycine soja. This rhizobium harbors nif genes on 150- to 200-megadalton plasmids. By passage on acridine orange plates, we obtained a mutant of R. fredii USDA 206 cured...

DRIVER (Chinese)

127

Enhancement or inhibition of insulin signaling by insulin receptor substrate 1 is cell context dependent.

Yamauchi, K; Pessin, J E

Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin...

DRIVER (Chinese)

128

Efficient procedure for transferring specific human genes into Chinese hamster cell mutants: interspecific transfer of the human genes encoding leucyl- and asparaginyl-tRNA synthetases.

Cirullo, R E; Wasmuth, J J; Dana, S

We have developed a simple and efficient procedure for transferring specific human genes into mutant Chinese hamster ovary cell recipients that does not rely on using calcium phosphate-precipitated high-molecular-weight DNA. Interspecific cell hybrids between human leukocytes and temperature-sensiti...

DRIVER (Chinese)

129

Effects of genomic context and chromatin structure on transcription-coupled and global genomic repair in mammalian cells

Tang, Moon-shong; Feng, Zhaohui; Chasin, Lawrence A.; Hu, Wenwei

It has been long recognized that in mammalian cells, DNA damage is preferentially repaired in the transcribed strand of transcriptionally active genes. However, recently, we found that in Chinese hamster ovary (CHO) cells, UV-induced cyclobutane pyrimidine dimers (CPDs) are preferentially repaired i...

DRIVER (Chinese)

130

Dissecting a wheat QTL for yield present in a range of environments from the QTL to candidate genes

S Quarrie; A Kaminska; Prof. Jeremy David Barnes

Previous studies with 95 bread wheat doubled haploid lines DHLs from the cross Chinese Spring CSxSQ1 trialled over 24 yearxtreatmentxlocations identified major yield quantitative trait loci QTLs in homoeologous locations on 7AL and 7BL expressed mainly under stressed and non-stressed conditions resp...

DRIVER (Chinese)

131

Differential expression of three alpha-tubulin genes in Chinese hamster ovary cells.

Henderson, G; Elliott, E M; Sarangi, F; Okayama, H; Ling, V

Chinese hamster ovary cells contain a complex family of ca. 16 unique alpha-tubulin sequences and a similar multiplicity of beta sequences. To examine which members of this multigene family are expressed, we constructed cDNA libraries from two Chinese hamster ovary cell lines according to the method...

DRIVER (Chinese)

132

Differential amplification and disproportionate expression of five genes in three multidrug-resistant Chinese hamster lung cell lines.

Borst, P; de Bruijn, M H; Biedler, J L; Van der Bliek, A M

At least five linked genes are amplified in the multidrug-resistant Chinese hamster ovary cell line CHRC5, selected with colchicine (A. M. Van der Bliek, T. Van der Velde-Koerts, V. Ling, and P. Borst, Mol. Cell. Biol. 6:1671-1678, 1986). We report here that only a subset of these, encoding the 170-...

DRIVER (Chinese)

135

Deprivation of a single amino acid induces protein synthesis-dependent increases in c-jun, c-myc, and ornithine decarboxylase mRNAs in Chinese hamster ovary cells.

Pohjanpelto, P; Hölttä, E

Genes of higher eucaryotic cells are considered to show only a limited response to nutritional stress. Here we show, however, that omission of a single essential amino acid from the medium caused a marked rise in the mRNA levels of c-myc, c-jun, junB and c-fos oncogenes and ornithine decarboxylase (...

DRIVER (Chinese)

136

Delay of vaccinia virus-induced apoptosis in nonpermissive Chinese hamster ovary cells by the cowpox virus CHOhr and adenovirus E1B 19K genes.

Ink, B S; Evan, G I; Gilbert, C S

The infection of vaccinia virus in Chinese hamster ovary (CHO) cells produces a rapid shutdown in protein synthesis, and the infection is abortive (R.R. Drillien, D. Spehner, and A. Kirn, Virology 111:488-499, 1978; D.E. Hruby, D.L. Lynn, R. Condit, and J.R. Kates, J. Gen. Virol. 47:485-488, 1980). ...

DRIVER (Chinese)

140

Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer.

McBride, O W; Ruddle, F H; Burch, J W

The Chinese hamster genes for thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75) and galactokinase (ATP:D-galactose 1-phosphotransferase, EC 2.7.1.6) have been cotransferred to mouse cells by chromosome-mediated gene transfer. Hamster metaphase chromosomes were incubated with mouse ...

DRIVER (Chinese)

141

Coordinate expression of amplified metallothionein I and II genes in cadmium-resistant Chinese hamster cells.

Griffith, J K

Recombinant DNA probes complementary to Chinese hamster metallothionein (MT)-1 and MT-2 mRNAs were used to compare MT gene copy numbers, zinc-induced MT mRNA levels, and uninduced MT mRNA levels in cadmium-resistant (Cdr) Chinese hamster ovary cell lines. Quantitative hybridization analyses determin...

DRIVER (Chinese)

142

Coordinate amplification of metallothionein I and II genes in cadmium-resistant Chinese hamster cells: implications for mechanisms regulating metallothionein gene expression.

Tesmer, J G; Munk, A C; Griffith, J K; Crawford, B D; Walters, R A; Griffith, B B; Enger, M D; Hanners, J L; Stallings, R L

We describe here the derivation, characterization, and use of clonal cadmium-resistant (Cdr) strains of the Chinese hamster cell line CHO which differ in their metallothionein (MT) induction capacity. By nondenaturing polyacrylamide gel electrophoresis, we showed that the stable Cdr phenotype is cor...

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143

Conservation of promoter sequence but not complex intron splicing pattern in human and hamster genes for 3-hydroxy-3-methylglutaryl coenzyme A reductase.

Luskey, K L

Regulation of the expression of 3-hydroxy-3-methyglutaryl coenzyme A (HMG-CoA) reductase is a critical step in controlling cholesterol synthesis. Previous studies in cultured Chinese hamster ovary cells have shown that HMG-CoA reductase is transcribed from a cholesterol-regulated promoter to yield a...

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144

Complete sequence of three alpha-tubulin cDNAs in Chinese hamster ovary cells: each encodes a distinct alpha-tubulin isoprotein.

Ling, V; Elliott, E M; Sarangi, F; Henderson, G

The genome of Chinese hamster ovary (CHO) cells contains a complex family of approximately 16 alpha-tubulin genes, many of which may be pseudogenes. We present here the complete cDNA sequences of three expressed alpha-tubulin genes; one of these genes has been identified only in CHO cells. The nonco...

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147

Common variants in FLNB/CRTAP, not ARHGEF3 at 3p are associated with osteoporosis in southern Chinese women

Li, GHY; Kung, AWC; Huang, Q

SUMMARY: We performed an association study of five candidate genes within chromosome 3p14-25 in 1,080 Chinese female subjects. Polymorphisms in FLNB/CRTAP are associated with bone mineral density (BMD) in Chinese. INTRODUCTION: Chromosomal region 3p14-25 has shown strong evidence of linkage to BMD i...

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149

Chromosome breakage at a major fragile site associated with P-glycoprotein gene amplification in multidrug-resistant CHO cells.

Hittelman, W N; Kuo, M T; Jiang, L X; Vyas, R C

Recent studies of several drug-resistant Chinese hamster cell lines suggested that a breakage-fusion-bridge mechanism is frequently involved in the amplification of drug resistance genes. These observations underscore the importance of chromosome breakage in the initiation of DNA amplification in ma...

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150

Cell cycle regulation of H2b histone octamer DNA-binding activity in Chinese hamster lung fibroblasts.

Maxson, R; Ito, M; Lee, A S; Sharma, A

The promoter regions of H2b histone genes contain a 14-base-pair element which includes the octamer ATTTGCAT. Mutational analysis has implicated the octamer element in the cell cycle-dependent expression of H2b histone genes. In this report, we address the question of whether the DNA-binding activit...

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152

Biochemical evidence for multiple independent emetine resistance genes in Chinese hamster cells.

Roufa, D J; Nielsen-Smith, K; Frahm, M; McGill, S

Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the r...

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153

Bayesian statistical analyses for presence of single genes affecting meat quality traits in a crossed pig population.

Janss, L.L.G.; Arendonk, J.A.M., van; Brascamp, E.W.

Presence of single genes affecting meat quality traits was investigated in F(2) individuals of a cross between Chinese Meishan and Western pig lines using phenotypic measurements on 11 traits. A Bayesian approach was used for inference about a mixed model of inheritance, postulating effects of polyg...

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Bayesian Statistical Analyses for Presence of Single Genes Affecting Meat Quality Traits in a Crossed Pig Population

Janss, LLG.; Brascamp, E. W.; Van-Arendonk, JAM.

Presence of single genes affecting meat quality traits was investigated in F(2) individuals of a cross between Chinese Meishan and Western pig lines using phenotypic measurements on 11 traits. A Bayesian approach was used for inference about a mixed model of inheritance, postulating effects of polyg...

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157

Association of BANK1 and TNFSF4 with systemic lups erythematosus in Hong Kong Chinese [Epub 9 Apr 2009]

Chang, YK; Yang, W; Zhao, M; Mok, CC; Chan, DTM; Wong, RWS; Lee, KW; Mok, TMY; Wong, SN; Ng, IOL; Lee, TL; Ho, MHK; Lee, PPW

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Assignment of genes encoding metallothioneins I and II to Chinese hamster chromosome 3: evidence for the role of chromosome rearrangement in gene amplification.

Hildebrand, C E; Stallings, R L; Longmire, J L; Munk, A C; Crawford, B D

Cadmium resistant (Cdr) variants with coordinately amplified metallothionein I and II (MTI and MTII) genes have been derived from both Chinese hamster ovary and near-euploid Chinese hamster cell lines. Cytogenetic analyses of Cdr variants consistently revealed breakage and rearrangement involving ch...

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164

Analysis of the genes involved in the insulin transmembrane mitogenic signal in Chinese hamster ovary cells, CHO-K1, utilizing insulin-independent mutants.

Brown, E; Mamounas, M; Luong, C L; Ross, S; Coulter, K; Carroll, G; Englesberg, E

CHO-K1 cells, wild type (WT), grow in a defined medium with insulin as the only essential hormone. When starved for insulin, these cells accumulate in G0/G1 stage. Insulin binding to its receptor stimulates DNA synthesis and cell division and induces an increase in abundance of mRNA for c-fos, c-jun...

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165

Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.

Urlaub, G; Nunberg, J H; Schimke, R T; Kaufman, R J; Chasin, L A

Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate: NADP+ oxidoreductase, EC 1.5.1.3) 200 times that of sensitive cel...

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166

Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.

Kaufman, R J; Schimke, R T

During stepwise increases in the methotrexate concentration in culture medium, we selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional to the number of dihydrofolate reductase gene copies (i.e., gene amplification). We studied the dihydr...

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167

Amplification and expression of human alpha-globin genes in Chinese hamster ovary cells.

Lau, Y F; Kan, Y W; Lin, C C

We studied the effects of gene amplification on human globin gene expression in Chinese hamster ovary cells. The normal human alpha-globin gene (N alpha 2) and a hybrid gene (M alpha G) containing the 5' promoter-regulator region of the mouse metallothionein gene and the human structural alpha 2-glo...

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168

A novel DNA damage-inducible transcript, gadd7, inhibits cell growth, but lacks a protein product.

Hollander, M C; Fornace, A J; Alamo, I

gadd7 cDNA was isolated from Chinese hamster ovary (CHO) cells on the basis of increased levels of RNA following treatment with UV radiation. The transcript for gadd7, as well as for four other gadd genes, was found to increase rapidly and coordinately following several different types of DNA damage...

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169

A 22-kd protein (sorcin/V19) encoded by an amplified gene in multidrug-resistant cells, is homologous to the calcium-binding light chain of calpain.

Hes, E; Van der Bliek, A M; Biedler, J L; Meyers, M B; Borst, P

We have previously shown that at least five linked genes are co-amplified and overexpressed in the multi-drug resistant (MDR) Chinese hamster ovary cell line CHRC5. We show here that one of these genes (class 4) codes for a small phosphorylated, cytosolic protein, sorcin/V19, known to be overproduce...

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Liu, Wei; 劉蔚

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