Sample records for 基因重组 (gene recombination)

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Targeted homologous recombination at the endogenous adenine phosphoribosyltransferase locus in Chinese hamster cells.

Seidman, M M; Adair, G M; Wilson, J H; Nairn, R S; Brotherman, K A; MacKinnon, C; Scheerer, J B

We have developed a system that permits analysis of targeted homologous recombination at an endogenous, chromosomal gene locus in cultured mammalian cells. Using a hemizygous, adenine phosphoribosyltransferase (APRT)-deficient, Chinese hamster ovary (CHO) cell mutant as a transfection recipient, we ...

DRIVER (Chinese)


Latent Gene Sequencing Reveals Familial Relationships among Chinese Epstein-Barr Virus Strains and Evidence for Positive Selection of A11 Epitope Changes

Rickinson, A. B.; Midgley, R. S.; McGeoch, D. J.; Bell, A. I.

Epstein-Barr virus (EBV) strains from the highly HLA-A11-positive Chinese population are predominantly type 1 and show a variety of sequence changes (relative to the contemporary Caucasian prototype strain B95.8) in the nuclear antigen EBNA3B sequences encoding two immunodominant HLA-A11 epitopes, h...

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Illegitimate recombination induced by DNA double-strand breaks in a mammalian chromosome.

Phillips, J W; Morgan, W F

We examined DNA double-strand-break-induced mutations in the endogenous adenine phosphoribosyl-transferase (APRT) gene in cultured Chinese hamster ovary cells after exposure to restriction endonucleases. PvuII, EcoRV, and StuI, all of which produce blunt-end DNA double-strand breaks, were electropor...

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Homology dependence of targeted recombination at the Chinese hamster APRT locus.

Scheerer, J B; Adair, G M

Using simple linear fragments of the Chinese hamster adenine phosphoribosyltransferase (APRT) gene as targeting vectors, we have investigated the homology dependence of targeted recombination at the endogenous APRT locus in Chinese hamster ovary (CHO) cells. We have examined the effects of varying e...

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Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.

de Saint Vincent, B R; Wahl, G M

Chinese hamster cells with a lesion in the CAD gene (cell line Urd-A) require exogenous uridine to survive. Uridine prototrophs could be isolated after introducing two recombinant plasmids containing overlapping fragments of a cloned Syrian hamster CAD gene. In contrast, no uridine prototrophs were ...

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Gene targeting in Chinese hamster ovary cells is conservative.

Pennington, S L; Wilson, J H

Two fundamentally different pathways for homologous recombination have been identified in mammalian cells. For most chromosomal recombination events, two copies of a homologous sequence recombine to yield two copies in the products; such events are said to be conservative because the number of copie...

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Gene recombination in X-ray-sensitive hamster cells.

Hamilton, A A; Thacker, J

Recombination was measured in Chinese hamster ovary (CHO-K1) cells and in the X-ray-sensitive mutants xrs1 and xrs7, which show a defect in DNA double-strand break repair. To assay recombination, pairs of derivatives of the plasmid pSV2gpt were constructed with nonoverlapping deletions in the gpt ge...

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