Sample records for 半乳糖 (galactose)

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Mutant hamster cells exhibiting a pleiotropic effect on carbohydrate metabolism.

Sun, N C; Chu, E H; Chang, C C

Sixty-seven galactose negative (Gal minus) clones were established from survivors after treatment of Chinese hamster lung (V79) cells with 5-bromodeoxyuridine and black light. The mutational origin of these clonal isolates was inferred on the basis of the previously demonstrated mutagenic action of ...

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Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line

Bakker, H.; Routier, F.; Oelmann, S.; Jordi, W.J.R.M.; Lommen, A.; Gerardy-Schahn, R.; Bosch, H.J.

Nucleotide-sugar transporters (NSTs) form a family of structurally related transmembrane proteins that transport nucleotide-sugars from the cytoplasm to the endoplasmic reticulum and Golgi lumen. In these organelles, activated sugars are substrates for various glycosyltransferases involved in oligo-...

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Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells

Baker, KN; Rendall, MH; Hills, AE; Hoare, M; Freedman, RB; James, DC

Chinese hamster ovary and murine myeloma NS0 cells are currently favored host cell types for the production of therapeutic recombinant proteins. In this study, we compared N-glycan processing in GS-NS0 and GS-CHO cells producing the same model recombinant glycoprotein, tissue inhibitor of metallopro...

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Isolation of a Clone of Chinese Hamster Ovary Cells Deficient in Plant Lectin-Binding Sites

Gottlieb, Charlene; Kornfeld, Stuart; Skinner, Sister Ann Marie

Ricin, a galactose-binding lectin with potent cytotoxic activity, was used to select a clone of Chinese hamster ovary cells with altered plant lectin-binding properties. The clone (15B) is 80-fold less sensitive to the toxic action of ricin than the parent line. In the absence of ricin, it grows bot...

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Internalization of ricin in Chinese hamster ovary cells.

Ray, B; Wu, H C

Internalization of ricin into Chinese hamster ovary cells has been investigated. Combined treatment with galactose and pronase at 0 degrees C resulted in a complete release of surface-bound [125I]ricin into the media. Galactose-pronase-resistant cell-bound [125I]ricin represents internalized ricin m...

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Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer.

McBride, O W; Ruddle, F H; Burch, J W

The Chinese hamster genes for thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC and galactokinase (ATP:D-galactose 1-phosphotransferase, EC have been cotransferred to mouse cells by chromosome-mediated gene transfer. Hamster metaphase chromosomes were incubated with mouse ...

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Chromosome Assignment of the Human Gene for Galactose-1-phosphate Uridyltransferase*

Sun, N. C.; Chu, E. H. Y.; Chang, C. C.

Somatic cell hybrids formed between galactose-negative mutant Chinese hamster cells and human lymphocytes were selected in galactose medium and analyzed for the concurrent presence of specific human chromosome(s) and human galactose-1-phosphate uridyltransferase. In one of the hybrid clones only a s...

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Characterization of cell surface carbohydrate receptors for Entamoeba histolytica adherence lectin.

Petri, W A; Ravdin, J I; Murphy, C F; Stanley, P

Binding and cytolysis of Chinese hamster ovary (CHO) cells by Entamoeba histolytica trophozoites is inhibitable by galactose (Gal) or N-acetyl-D-galactosamine (GalNAc). To better define the carbohydrate receptor for E. histolytica, we compared the binding and cytolytic target properties of 10 CHO gl...

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