Sample records for luminol
from WorldWideScience.org

Sample records 1 - 8 shown.



1

A quimiluminescência como ferramenta analítica: do mecanismo a aplicações da reação do luminol em métodos cinéticos de análise/ Chemiluminescence as an analytical tool: from the mechanism to applications of the reaction of luminol in kinetic based methods

Ferreira, Ernesto Correa; Rossi, Adriana Vitorino
2002-11-01

Resumo em inglês Relevant aspects of proposed mechanisms of the chemiluminescent reaction of luminol are presented and commented to emphasize its perspectives for kinetic analysis. A careful search for analytical applications of this reaction is discussed in order to point out new trends of the studies. Kinetic analysis using the luminol reaction is proposed to be a very attractive due to the good performance of the reaction in analytical applications and the positive characteristics of k (mais) inetic analysis, such as low cost and sensibility. It is pointed out that kinetic analysis using the chemiluminescent reaction of luminol should be encouraged.

Scientific Electronic Library Online (Portuguese)

2

Determinação de paracetamol pela inibição da reação quimiluminescente do luminol-hipoclorito de sódio em um sistema de análise em fluxo empregando o conceito de multicomutação/ Determination of paracetamol using a flow injection analysis with multicommutation and chemiluminescence detection

Oliveira, Geiser G.; Janegitz, Bruno C.; Batistão, Marina B.; Salami, Fernanda H.; Fatibello-Filho, Orlando; Leite, Oldair D.
2009-01-01

Resumo em inglês A flow injection chemiluminescence method for the determination of paracetamol in pharmaceutical formulations is described. It is based on the consumption of the sodium hypochlorite by paracetamol and decreases of the analytical signal. The analytical curve was linear in the paracetamol concentration range from 5.0 x 10-6 to 5.0 x 10-5 mol L-1, with a detection limit of 1.8 x 10-6 mol L-1. The RSDs were 2.0 and 1.2% respectively for 2.0 x 10-5 and 4.0 x 10-5 mol L-1 paracetamol solutions (n = 10) and a sampling frequency of 180 h-1 was obtained.

Scientific Electronic Library Online (Portuguese)

3

The synthesis of a water-soluble derivative of rutin as an antiradical agent

Pedriali, Carla Aparecida; Fernandes, Adjaci Uchoa; Bernusso, Leandra de Cássia; Polakiewicz, Bronislaw
2008-01-01

Resumo em inglês The purpose of this study was to synthesize a water-soluble derivative of rutin (compound 2) by introducing carboxylate groups on rutin's sugar moiety. The rutin derivative showed an almost 100-fold solubility increase in water. The antiradical capacity of compound 2 was evaluated using the luminol/AAPH system, and the derivative's activity was 1.5 times greater than that of Trolox®. Despite the derivative's high solubility in water (log P = -1.13), lipid peroxidation of (mais) brain homogenate membranes was very efficiently inhibited (inhibition values were only 19% lower than the inhibition values of rutin).

Scientific Electronic Library Online (Portuguese)

4

Um experimento de análise em fluxo envolvendo reações enzimáticas e quimiluminescência/ A flow analysis experiment involving enzymatic reactions and chemiluminescence

Leite, Oldair Donizeti; Fatibello-Filho, Orlando; Rocha, Fábio R. P.
2004-04-01

Resumo em inglês An experiment is proposed to introduce some fundamentals of flow analysis, chemiluminescence and kinetic monitoring of enzymatic reactions in undergraduate courses. Chemiluminescence detection is performed with a simple spectrophotometer equipped with a lab-made spiral flow cell constructed from a polyethylene tube. The hydrogen peroxide produced by the glucose oxidation in the presence of glucose oxidase is continuously monitored by the reaction with luminol in alkaline (mais) media in a flow injection system. The exercise allows also the discussion of important analytical features and the comparison with different optical methods of analysis.

Scientific Electronic Library Online (Portuguese)

5

Quimiluminescência orgânica: alguns experimentos de demonstração para a sala de aula/ Organic chemiluminescence: some classroom demonstration experiments

Albertin, Ricardo; Arribas, Marco A. G.; Bastos, Erick L.; Röpke, Sascha; Sakai, Patricia N.; Sanches, Adrey M. M.; Stevani, Cassius V.; Umezu, Ilka S.; Yu, Joana; Baader, Wilhelm J.
1998-11-01

Resumo em inglês The generation of "cold light", visible to the human eye, by chemical reactions has attracted the attention of the scientific community since the beginning of this century. Besides the academic interest in the elucidation of the mechanisms of excited state formations, many chemiluminescence reactions have found widespread analytical applications. Moreover, the phenomenon of chemiluminescence can also be used as a tool in undergraduate and college teaching. In this article (mais) , we describe several known chemiluminescence demonstrations, which are suitable for school teaching. The main objective of this work is to produce didactic material in Portuguese to stimulate Brazilian secondary and high school teachers to use these experiments in the classroom. The demonstrations include singlet oxygen emission, the luminol reaction, oscillating chemiluminescence and the peroxyoxalate reaction.

Scientific Electronic Library Online (Portuguese)

6

Avaliação bioquímica e ultraestrutural da interação de imunocomplexos de IgG com leucócitos polimorfonucleares: efeito de antioxidantes naturais/ Biochemical and ultrastructural evaluation of the interaction between IgG immunecomplexes and polymorphonuclear leukocytes: effect of natural antioxidants

Oliveira, Carlos Alberto de; Azzolini, Ana Elisa Caleiro Seixas; Silva, Silvana Chedraoui; Kanashiro, Alexandre; Kabeya, Luciana Mariko; Azevedo, Ana Paula Gomes Braga de; Gonçalves, Cristiane Fernandes de Freitas; Corediro, Daniel Sivieri; Leitão, Denise Pimenta da Silva; Gaspar, Lorena Rigo; Souza, Pablo Leonardo Viana de; Valim, Yara Maria Lucisano; Mantovani, Bernardo
2002-01-01

Resumo em português A produção de espécies reativas de oxigênio (EROs) por leucócitos polimorfonucleares (LPMNs) durante a fagocitose é essencial para a defesa do organismo contra microrganismos invasores. Entretanto, em algumas doenças ocorre a deposição de imunocomplexos, o que leva à produção e liberação excessiva dessas EROs, e conseqüente lesão nos tecidos hospedeiro. Neste trabalho avaliamos o efeito de substâncias naturais (quercetina e 7-aliloxicumarina) sobre a prod (mais) ução de EROs por LPMNs de coelho, estimulados com imunocomplexos de imunoglobulina G (ICIgG), empregando o ensaio de quimioluminescência (QL) dependente de luminol (QL lum) e de QL dependente de lucigenina (QL luc). A interação entre ICIgG e LPMNs, mediada pelo receptor de membrana Fcgama, foi observada por microscopia eletrônica de transmissão, pela intensa marcação dos ICIgG com partículas de ouro coloidal. Os resultados obtidos indicaram que as substâncias analisadas inibiram a QL lum e a QL luc e que tais efeitos não parecem estar relacionados com a inibição da fagocitose, conforme observado no ensaio por microscopia eletrônica. Resumo em inglês The generation of reactive oxygen species (EROs) by polymorphonuclear leukocytes (LPMNs) during phagocytosis plays a key role in the organism defense against invading microorganisms. However, in some diseases the immune complexes deposition occurs, that causes an increase in the production and release of EROs. Consequently, the host tissue is damaged. In this work we studied the effect of natural compounds (quercetin and 7-allyloxycoumarin) on EROs generation by rabbit (L (mais) PMNs) stimulated with immunecomplexes (IC) of chicken egg albumin (antigen) and class G immuneglobulins (IgG) - ICIgG. The EROs production was measured by chemiluminescence assay (QL) enhanced by luminol (QL lum) or by lucigenin (QL luc). The interaction between ICIgG (conjugated with colloidal gold) e LPMNs, mediated by Fcgamma membrane receptor, was observed by transmission electron microscopy. The results indicated that the studied compounds inhibited QL lum and QL luc. They also suggest that such effects are not related to phagocytosis inhibition, as observed by the electron microscopy assay.

Scientific Electronic Library Online (Portuguese)

7

Potencial antioxidativo da lágrima de adultos jovens/ Tear antioxidant potential in young adults

Gus, Patrícia Ioschpe; Belló-Klein, Adriane; Llesuy, Susana; Quinto, Guilherme Goulart; Matos, Guilherme Herrmann; Bechara, Samir Jacob
2006-08-01

Resumo em português OBJETIVO: Estudar os antioxidantes da lágrima humana, estimulada pelo corte de cebola e a possível influência dos hábitos de vida sobre estas medidas. MÉTODOS: A amostra consistiu de dez adultos jovens, que responderam questionário sobre o hábito de fumar, de ingerir bebidas alcoólicas, frutas, vegetais e cereais, de usar vitaminas e/ou drogas e de praticar exercícios. O potencial reativo antioxidante total (TRAP) foi analisado por meio da quimioluminescência do (mais) luminol, a superóxido dismutase (SOD) foi medida pela inibição do piragolol e a absorbância de H2O2 a 240 nm foi utilizada para identificar a catalase. RESULTADOS: A média ± DP dos valores de potencial reativo antioxidante total foi 33,8±11,5 µM e de superóxido dismutase foi 10,8±1,9 U/mL. Não foi identificada atividade da catalase. Detectou-se associação entre a prática regular de exercícios e aumento nos valores de potencial reativo antioxidante total (p=0,021), com diferença de 18,6 µM entre os indivíduos que se exercitavam pelo menos uma vez por semana e os sedentários. Sexo masculino e aumento na concentração de potencial reativo antioxidante total também se associaram estatisticamente (p=0,013), com diferença de 16,3 µM entre os sexos. Houve associação entre hábito tabágico e aumento na superóxido dismutase (p=0,041), com diferença de 3,3 U/mL entre fumantes de mais de cinco cigarros/dia e não fumantes. O uso de vitamina C também demonstrou associação com a superóxido dismutase (p=0,018); a diferença para os que tomavam vitamina C foi de 3,3 U/mL a mais. CONCLUSÃO: Os antioxidantes do lacrimejamento reflexo foram mensuráveis em adultos jovens, e diferentes variáveis parecem ter influenciado nos resultados. Resumo em inglês PURPOSE: To study the antioxidant status of human tears, stimulated by onion fumes and the possible influence of the life habits thereon were measured. METHODS: Subjects were ten healthy young adults, who answered a questionnaire about smoking, alcohol ingestion, fruit, vegetable, cereal, and vitamin intake and/or intake of other drugs, and physical exercise habits. Chemoluminescensce of luminol was used to analyze the total reactive antioxidant potential (TRAP), inhibiti (mais) on of piragollol was used to measure superoxide dismutase (SOD) and absorbance of H2O2 at 240 nm was used to identify catalase. RESULTS: Mean ± SD value for total reactive antioxidant potential was 33.8±11.5 µM and for superoxide dismutase 10.8±1.9 U/mL. Catalase was not identified. Regular exercise was associated with increased total reactive antioxidant potential values (p=0.021), with a difference of 18.6 µM between individuals who exercise at least once a week and sedentary individuals. Male gender and total reactive antioxidant potential values were statistically associated (p=0.013), with a difference of 16.3 µM between genders. There was an association between smoking and increased superoxide dismutase values (p=0.041), with a difference of 3.3 U/mL between smokers of more than five cigarettes/day and non-smokers. Also, vitamin C intake and superoxide dismutase values were associated (p=0.018); the difference for vitamin C takers was 3.3 U/mL. CONCLUSION: Reflex tearing antioxidants were measurable in healthy young adults, and different variables apparently influenced their values.

Scientific Electronic Library Online (Portuguese)

8

Desenvolvimento de um dispositivo de baixo custo para medidas por quimiluminescência/ Development of a low cost device for chemiluminescence measurements

Borges, Eduardo P.; Fernandes, Elizabeth N.; Rocha, Fábio R. P.; Reis, Boaventura F.
2002-12-01

Resumo em inglês A simple and low cost device (ca. US$ 150) that comprises two photodiodes fixed in lab-made Perspex flow cell is proposed for chemiluminescence measurements. The characteristics of the device (large observation window and reduced thickness) allow maximizing the amount of the emitted radiation detected. A sensitivity improvement of ca. 50 % was observed by employing two photodiodes for signal measurements. The performance of the device was assessed by the oxidation of lumi (mais) nol by hydrogen peroxide, yielding a linear response within the range of 2.50 to 500 µmol L-1 H2O2. The detection limit was estimated as 0.8 µmol L-1 hydrogen peroxide which is comparable with those obtained by using equipments based on photomultipliers.

Scientific Electronic Library Online (Portuguese)