Resumo em português Especiação química é definida como a determinação da concentração de diferentes formas físico-químicas individuais de um elemento, que em conjunto constitui a concentração total do mesmo, permitindo avaliar o aproveitamento do elemento no processo absortivo. As carnes bovinas e de aves são de grande importância na alimentação, assim, este trabalho tem como objetivo avaliar os teores de cobre em diferentes extratos obtidos pela aplicação da extração seq (mais) üencial de carnes in natura, processadas termicamente e congeladas por 30 dias. Os teores de cobre foram determinados através de espectroscopia de absorção atômica. Os dados foram tratados estatisticamente através do teste de Grubs e t de Student. Verificou-se que o cobre encontra-se na maioria das amostras estudadas, em no mínimo duas espécies químicas diferentes. As frações predominantes encontradas foram as representadas pela fração iônica, a qual enfraquece a ligação do metal com compostos orgânicos e sulfetos e promove a dissolução de silicatos e minerais. Observou-se que o processo de cocção e o congelamento diminuem os teores totais e influenciam nas formas químicas do metal. Estudos posteriores que identifiquem estas formas químicas do cobre podem favorecer na avaliação da biodisponibilidade do mesmo. Resumo em inglês Chemical specification is defined as determining the concentration of different forms of physicist-chemistry individuals of an element, which together consist of the total concentration, and whereby the element in the absorbed process can be evaluated. Beef and chicken are of great importance in food, and therefore the aim of this work was to evaluate copper in different extracts obtained from applying sequential extraction of meat, thermally processed and frozen for 30 d (mais) ays. Copper was determined by using an atomic absorption spectroscopy. The data was dealt with statistically by using the Grubs and t Student test. Copper was found in most of the studied samples and at least two different chemical species. The predominant fractions found were represented by the ionic fraction, which weakens the metal with organic composites and promotes the dissolution of silicates and minerals. It was observed that the thermal and freezing process diminishes the total contents and influences the chemical species of the metal. Studies to identify these chemicals of copper can be be favourable when evaluating the biodisponability.
Resumo em inglês In the beginning of this work a brief idea was given on what is understood as "clay", on the scientific methods applied to the study of the matter "clay" as well as on its conception with regard to the edaphic studies. A description is given of the methods of total and natural mechanical analyses which were employed in the agro-geological Department. The work is illustrated by a graphic showing the fractions of the mechanical analysis of the chief great soil types of the (mais) State of São Paulo. The fraction clay is then studied by chemical way in the following sequence : 1.° total chemical analysis, 2.° partial chemical analysis, 3.° chemical analysis of the Helmholz layer and 4.° study of the fixation of the ion Ca+ + in the already mentioned Helmholz layer. The first analysis separates and attacks the hydrated aluminium silicates, the hydrated oxyds of iron and aluminium, manganese, etc. In this analysis the relationship between silica and sesquioxyds was determined. The second type of attack or chemical analysis does not affect the hydrated silicates of aluminium, all others go in dissolution. In this analysis were determined chiefly the sesquioxyds in their free form in the clay complex. For the study of the Helmholz layer, i.e. the determination of changeable ions, solutes of NH4Cl N/l, HNO3 N/5, KCl N/l, etc. were used, percolation of these liquids being made through a layer of air dried soil. The relationship between soil and liquid was always 1:10. In this analysis, principally the cations K+, Ca++, Mg++, Al+ + +, H+ etc. were determined. With such determinations the calculations of the following values were obtained: S = sum of the changeable bases in ME; T = total value in ME of the sorptive power of the complex in each 100 g dried soil at 110° C. and V = percentage of saturation of the complexes with bases. Finally, the qualitative radiographic analysis of the fraction clay was carried out by means of the Debye-Scherrer-Hull method. For these studies the Siemens-Berlin apparatus was used, the transformer of which runs with all0-220 volts 60-cycle alternating current. The bulb has a copper anti-cathode and is fed by a current of 13 m.A. of intensity and the difference of potential is of 30,000, 35,000 and 47,000 volts. The flash of X-rays goes through the nickel filter in order to free it of the b irradiations which are deleterious. In this way, inciding vertically on the preparation, radiations CuK a with l = 1,539 A are obtained. The sensible film used was a Lawe-Film-Agfa, with an exposure going from 11/2 to 2 hours with 35,000 V. The chassis has a diameter of 57 mm. More than 500 radiographs of soil samples were taken, of those proceding from all districts of the State, 79 of which are presented in the present work. With this material the different constituents of the clay minerals contained in our soils were known. Thus, in the soils over the Archean geological formation, kaolinite is represented most frequently and abundantly ; the hydrargillite also appears commonly. The soils over the Glacial formation contain the same representatives and oscillations as those just mentioned. In the Corum-batai formation, quartz is the most important constituent. The Botucatu formation (lava) is that which gave origin to our legitimate red soils ; the mineralogical clay constituents of these soils are : 1.° large amount of hydrated oxyds of iron up to 35% of Fe2O3 for each 100 g of soil at 110° C. ; 2.° hydrargillite and 3.° kaolinite. The amount of the latter in the clay complex ranges between 9 and 25%. The sandy soils existing in the geological formations of lower and upper Baurú bear in their clays the following mineralogical constituents : 1.° kaolinite, 2.° montmorrillonitic type (on the upper Baurú only), 3.° hydrargillite (more common on the lower Baurú) and 4.° quartz, generally, in small percentages. These researches made it evident that the constituents of the clay fraction of our soils commonly have a small sorptive power related to the cations; it follows hence that they do not show a great power of retention of manure with the exception of the montmorrillonitic type.