Sample records for TRANSPOSONES (transposons)
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Sample records 1 - 20 shown. Select sample records:



2

Genome-Wide Analysis of the ‘‘Cut-and-Paste’’ Transposons of Grapevine

Benjak, Andrej; Forneck, Astrid; Casacuberta, Josep M.
2008-09-03

Digital.CSIC (Spain)

3

Genome-wide analysis of the "cut-and-paste" transposons of grapevine

Benjak, Andrej; Forneck, Astrid; Casacuberta, Josep M.
2008-09-03

Digital.CSIC (Spain)

5

cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

Gutiérrez de Diego, Juana; Rodríguez García, David; Rodríguez Lorenzo, José Luis; Grappin, Philippe; Cervantes, Emilio
2006-03-01

Digital.CSIC (Spain)

6

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet(L) and Chromosomally Located Transposon-Associated tet(M), Coexist in Lactobacillus sakei Rits 9

Ammor, Mohammed Salim; Gueimonde Fernández, Miguel; Danielsen, Morten; Zagorec, Monique; H. A. M. van Hoek, Angela; González de los Reyes-Gavilán, Clara; Mayo Pérez, Baltasar; Margolles Barros, Abelardo
2008-01-11

Digital.CSIC (Spain)

7

Integrones: los coleccionistas de genes/ Integrons: gene collectors

Di Conza, J. A.; Gutkind, G. O.
2010-02-01

Resumen en español Los integrones son estructuras genéticas que han despertado gran interés, debido a que algunos de ellos vehiculizan genes de resistencia a los antimicrobianos. Están formados por un fragmento que codifica una integrasa (intI) y, a continuación, una secuencia attI a la que se unen los genes en casetes que codifican diferentes mecanismos de resistencia. Dentro de intI, en su extremo 3´, hay una secuencia promotora Pc a partir de la cual se transcriben los casetes de re (mas) sistencia integrados, ya que estos genes carecen de promotor. Sin embargo, estos casetes presentan una secuencia específica denominada attC, la cual es reconocida por la integrasa que se une, por recombinación, a la secuencia attI del integrón en la orientación adecuada para su expresión. Los integrones se han clasificado según la secuencia de su integrasa, pero en la actualidad se prefiere clasificarlos según su localización. Se habla, en general, de "integrones móviles" para referirse a aquellos asociados a secuencias de inserción, transposones y/o plásmidos conjugativos, los que en su mayoría median mecanismos de resistencia, y de "superintegrones", de localización cromosómica y con grandes arreglos de genes en casetes. Los integrones móviles de clase 1 son los más abundantes en aislamientos clínicos y suelen estar asociados a transposones del subgrupo Tn21, seguidos por los de clase 2, derivados principalmente de Tn7. Estos elementos no son móviles por sí mismos, pero su asociación con elementos que sí lo son facilita su transferencia horizontal, lo que explica su amplia difusión entre las bacterias. Esta revisión intenta recopilar la información disponible acerca de los integrones móviles descritos en Argentina hasta la fecha. Resumen en inglés Integrons gained great interest due to their participation in resistance gene recruitment and expression. Their basic structure includes a fragment that encodes an integrase (intI) followed by a recognition sequence (attI) into which they may incorporate gene cassettes (encoding resistance mechanisms). A promoter (Pc) embedded within the integrase gene controls the transcription of integrated resistance markers, as these genes do not have their own promoters. When in cass (mas) ettes, resistance genes are flanked by specific sequences (attC), which are recognized by the integrase that, by site specific recombination, incorporates them after attI in proper orientation for their expression. In the past, integrons were classified according to their sequence homology; currently they are classified according to their location. In general, they are divided into "mobile" integrons (those associated with insertion sequences, transposons and/or plasmids, being most of them associated with resistance mechanisms), and chromosomally-located "super" integrons with large arrangements of cassette genes. "Mobile" class 1 integrons are the most abundant in clinical isolates and are generally associated with Tn21 subgroup transposons, followed by class 2, derived primarily from Tn7. These elements are not mobile themselves, but their association with mobile platforms that facilitate horizontal transfer, explains their wide distribution among bacteria. This review also attempts to describe the mobile integrons described so far in Argentina.

Scientific Electronic Library Online (Spanish)

8

Grandes alcances de los RNAs pequeños RNA de interferencia y microRNA/ Great potential of small RNas: RNA interference and microRNA

Vázquez-Ortiz, Guelaguetza; Piña-Sánchez, Patricia; Salcedo, Mauricio
2006-08-01

Resumen en español El RNA de doble cadena puede inducir un silenciamiento secuencia-específico en eucarionte. Este proceso de silenciamiento se inicia cuando el RNAdc largo es procesado a RNA pequeño de 21 a 26 nucleótidos mediante la enzima RNAsa III Dicer. Estos RNA pequeños se incorporan a complejos efectores de silenciamiento, que son guiados a secuencias complementarias blanco. Existen diferentes tipos de silenciamiento, cuyas diferencias se basan principalmente en la naturaleza de (mas) la secuencia blanco y en la composición proteica de los complejos efectores. La ruta del RNA de interferencia (RNAi) se inicia cuando Dicer genera pequeños RNA de interferencia (siRNA) que se unen por complementariedad al mRNA para su degradación, utilizando el complejo RISC. De manera natural, los siRNA se originan de transposones y virus que producen RNAdc durante su replicación, así como también de otras secuencias repetidas transcritas bidireccionalmente. Algunas de las enzimas que conforman la maquinaria del RNAi como Dicer, entre otras, son codificadas por familias multigénicas en varias especies y también participan en otros mecanismos de silenciamiento mediado por RNA. Los microRNA son otros RNA pequeños que pueden inducir silenciamiento al unirse al mRNA. Éstos se generan de manera general cuando Dicer procesa estructuras de horquilla compuestas de regiones no codificantes, en genomas de plantas y animales. Los miRNA se incorporan a un complejo similar a RISC y, dependiendo de su grado de complementariedad con el mRNA blanco, pueden tener represión traduccional o bien digerir el mRNA. El silenciamiento mediado por miRNA es esencial para el desarrollo de plantas y animales. La inducción artificial del RNAi mediante siRNA o miRNA ha sido adoptada como una herramienta para inactivar la expresión génica, tanto en células en cultivo como en organismos vivos. En esta revisión se muestra el gran progreso en el entendimiento de los mecanismos que participan en la regulación génica mediada por RNA en animales y detalla algunos esfuerzos actuales para encauzar a estos mecanismos como una herramienta en la investigación y como posible terapia en enfermedades. Resumen en inglés Double-stranded RNA (dsRNA) induces a sequence-specific silencing in eukaryotic cells. This silencing process beggins when long dsRNA is cleaved to 21 to 26 long small RNA by means of the RNAse III-type enzyme Dicer. These small dsRNA are included into silencing effector complexes, that are targeted to complementary sequences. Small RNA dependent gene silencing can be achieved by distinct mechanisms based depending mainly on the nature of target sequences and on the prote (mas) ins present in the effector complex. The route of interference RNA (RNAi) begins when Dicer yields small interference RNA (siR-NA) that bind to complementary mRNA for its degradation, forming the RISC complex. siRNA are naturally formed from transposons and dsRNA viruses during its replication, as well as from other bidirectional transcribed repetitive sequences. Some of the enzymes thar are part of the RNAi machinery, including Dicer, are encoded by multigene families in many species, that also play a role in other mechanisms of RND-dependent gene silencing. MicroRNA's (miRNA) are other small RNA's that can induce gene silencing at the mRNA level. These are formed in a general manner when Dicer process hairpin structures resulting from the transcription of non-coding sequences from plant and animal genomes. miRNA's are integrated into a RISC-like complex, after which, depending on their degree of complementarity with target mRNA, can either repress translation or induce mRNA degradation. miRNA-dependent silencing is essential for the development of multicellular organisms. Artificial RNAi induction by means of siRNA or miRNA is being used as a tool to inactivate gene expression in culture cells and in living organisms. This review focuses on the progress in the understanding of the mechanisms involved in gene regulation by RNA in animals and details some current efforts to apply theses phenomena as a tool in research and in the therapeutic of human diseases.

Scientific Electronic Library Online (Spanish)

9

Integrones y cassettes genéticos de resistencia: estructura y rol frente a los antibacterianos/ Integrons and resistance gene cassettes: structure and role against antimicrobials

González R, Gerardo; Mella M, Sergio; Zemelman Z, Raúl; Bello T, Helia; Domínguez Y, Mariana
2004-05-01

Resumen en inglés Bacteria have developed sophisticated and successful genetic mechanisms to evade the action of antimicrobials. Bacterial multiresistance has caused serious problems in the treatment of nosocomial infections. Integrons and gene cassettes are considered the main genetic elements in the evolution of plasmids and transposons that actively participate in the mobilization of genes, codifying different bacterial resistance mechanisms. This article reviews the historical and stru (mas) ctural aspects of integrons and resistance gene cassettes and the presence of these structures in Gram negative bacteria isolated from Chilean hospitals in the last ten years (Rev Méd Chile 2004; 132: 619-26)

Scientific Electronic Library Online (Spanish)

10

Aminoglucósidos-aminociclitoles: Características estructurales y nuevos aspectos sobre su resistencia/ Aminoglycosides-aminocyclitols: Structural characteristics and new aspects on resistance

Mella M, Sergio; Sepúlveda A, Marcela; González R, Gerardo; BelloT, Helia; Domínguez Y, Mariana; Zemelman Z, Raúl; Ramírez G, César
2004-12-01

Resumen en inglés The aminoglycoside-aminocyclitol antibiotics constitute one of the antibacterial agent families with greater activity upon aerobic Gram-negative bacilli. These compounds are formed by the combination of one amino-cyclic alcohol (aminocyclitol) and aminosaccharides (aminoglycosides) linked by glycosidic bonds. The strong bactericidal activity exhibited for these compounds is not only explained by their ability to inhibit the protein synthesis, but also by pleiotropic effec (mas) t altering the permeability of cytoplasmatic membrane. The penetration of these antibiotics to the bacterial cells is mediated by three well defined phases, being the two latest dependant of the proton-motive force. This fact explains that this kind of compounds have no antibacterial activity upon anaerobic bacteria. Bacterial resistance to aminoglycosides is mainly due to aminoglycoside-modifying enzymes (AME), which are commonly encoded by extrachromosomal genetic elements as are plasmids and transposons. Nevertheless, new mechanisms of resistance and genetic elements participating in the resistance to these compounds have been identified. Thus, recently the methylation of the 16S rRNA binding the aminoglycosides has been described. On the other hand, the gene cassettes acquire an increasing importance, because they can host a varied of families of antibiotic resistance genes, including the aminoglycoside-aminocyclitols. These gene cassettes are associated to integrons, which are able to integrate and express these antibiotic resistance determinants

Scientific Electronic Library Online (Spanish)

11
14

The neglected intrinsic resistome of bacterial pathogens

Fajardo, A.; Martínez-Martín, Nadia; Mercadillo, María; Galán, Juan Carlos; Ghysels, Bart; Matthijs, Sandra; Cornelis, Pierre; Wiehlmann, Lutz; Tümmler, Burkhard; Baquero, Fernando; Martínez, José L.
2008-02-20

Digital.CSIC (Spain)

16

Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

González, Víctor M.; Benjak, Andrej; Hénaff, Elizabeth Marie; Mir, Gisela; Casacuberta, Josep M.; García-Mas, Jordi; Puigdomènech, Pere
2010-11-12

Digital.CSIC (Spain)

17

Recent amplification and impact of MITEs on the genome of grapevine (Vitis vinifera L.)

Benjak, Andrej; Boué, Stéphanie; Fornaeck, Astrid; Casacuberta, Josep M.
2009-05-20

Digital.CSIC (Spain)

19

Molecular cloning and expression in different microbes of the DNA encoding Pseudomonas putida U phenylacetyl-CoA ligase. Use of this gene to improve the rate of benzylpenicillin biosynthesis in Penicillium chrysogenum

Miñambres Rodríguez, Baltasar; Martínez Blanco, Honorina; Olivera, Elías R.; García, Belén; Díez, Bruno; Barredo, José L.; Moreno, Miguel A.; Schleissner, Carmen; Salto, Francisco; Luengo, José M.
1996-01-01

Digital.CSIC (Spain)

20
22

Heterologous Expression and Identification of the Genes Involved in Anaerobic Degradation of 1,3-Dihydroxybenzene (Resorcinol) in Azoarcus anaerobius

Darley, Paula I.; Hellstern, Jutta A.; Medina-Bellver, Javier I.; Marqués, Silvia; Schink, Bernhard; Philipp, Bodo
2007-05-01

Digital.CSIC (Spain)

23

From a Short Amino Acidic Sequence to the Complete Gene

Miñambres Rodríguez, Baltasar; Olivera, Elías R.; García, Belén; Naharro, Germán; Luengo, José M.
2000-01-01

Digital.CSIC (Spain)

24

Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis

Ocampo Sosa, Alain Antonio; García Lobo, Juan María
2008-01-24

Digital.CSIC (Spain)