Sample records for TRANSFERASAS (transferases)
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1

Expresion del receptor de esteroides y xenobioticos (SXR) y del gen de multirresistencia drogas (MDR1) y de los polimorfismos de las enzimas GSTs, SULTs y CYP en tumores vesicales profundos, análisis de su expresión y correlación con otros factores pronósticos/ Steroid and Xenobiotic Receptor (SXR), Multidrug Resistance Gene (MDR1) and GSTs, SULTs AND CYP polymorphism expression in invasive bladder cancer, analysis of their expression and correlation with other prognostic factors

Rioja Zuazu, J.; Bandrés Elizalde, E.; Rosell Costa, D.; Rincón Mayans, A.; Zudaire Bergera, J.; Gil Sanz, MªJ.; Rioja Sanz, L.A.; García Foncillas, J.; Berián Polo, J.Mª
2007-12-01

Resumen en español Introducción: El receptor de esteroides y xenobióticos SXR se ha demostrado su activación por parte de numerosos medicamentos, incluidos potentes inductores del citocromo P450, como la rifampicina y el cotrimazol. La función del SXR es bien conocida, y consiste en regular de manera positiva la trascripción del citocromo P450 3A4 (CYP3A4) y el gen de multirresistencia a drogas (multidrug resistance gene) MDR1, se considera una llave clave en el mecanismo regulador del (mas) metabolismo de los xenobióticos encontrándose involucrado en todas las fases de detoxificación Múltiples enzimas involucradas en el metabolismo y la degradación de hidrocarburos policíclicos aromáticos (PAH) son polimórficas en humanos, incluyendo la glutation S-transferasa (GSTs), N-acetiltransferasa (NATs), sulfotransferas (SULTs)1A1 y el citocromo p450 (CYP)1B1. Objetivos: Los objetivos que nos hemos planteado son los siguientes: 1. Analizar la expresión del factor de trascripción SXR y del MDR1 en vejiga mediante RT-PCR en tiempo real, tanto en vejiga tumoral como vejiga normal. 2. Analizar la relación de los factores clínicos y patológicos con la expresión del SXR y del MDR1. 3. Analizar la expresión de los polimorfismos de CYP1B1, GSTM1 GSTT1 y SULT1A1, y su correlación con distintos factores clínico patológicos y moleculares. Material y Métodos: De manera prospectiva se calculó un tamaño muestral necesario para este estudio. Se incluyeron 67 pacientes de dos instituciones distintas (Hospital Universitario Miguel Servet (49 HUMS) y Clínica Universitaria de Navarra (18 CUN)), diagnosticados de cáncer vesical infiltrante y tratados mediante cistectomía radical, se le realizó la determinación de la expresión de SXR y MDR1 mediante PCR cuantitativa en tiempo real, así como de los polimorfismos CYP1B1, GSTM1 GSTT1 y SULT1A1 mediante RFLP (restricción de la longitud del fragmento del polimorfismo). Se correlaciona mediante tablas de contingencia la correlación con el resto de los factores pronósticos. Resultados: La media de seguimiento de los pacientes fue de 23,7 meses, con una mediana de 28,26 meses. De los 67 pacientes estudiados, 31 pacientes (46,3%) presentaron progresión de la enfermedad, bien en forma de recidiva local, metástasis a distancia o ambos, con un tiempo medio a recidiva de 12,4 meses, mediana de 10 meses, con un rango de 1,1 mes a 31,9 meses. 36 pacientes (53,7%) no presentaron evidencia de progresión de la enfermedad. El receptor de esteroides y xenobióticos SXR así como el gen de multirresistenia a drogas (Multidrug resistance gene (MDR1)), se expresan en vejiga normal (0,94ΔCt y 0,94ΔCt) y en vejiga tumoral de la pieza de cistectomνa (1,09 ΔCt y 0,45 ΔCt). Hemos analizado su expresiσn de manera cuantitativa y de manera cualitativa. La expresiσn de SXR se correlaciona con la presencia de carcinoma in situ (p=0,024), infiltraciσn vasculo-linfαtica (p=0,05) mientras que MDR1 se correlaciona con la presencia de infiltraciσn vasculo linfαtica (p=0,05) A su vez ambos la presencia de ambos factores se correlaciona entre ellos (p=0,011) Los polimorfismos: CYP1B1, GSTM1, GSTT1 y SULT1A1, se expresan en vejiga pero su expresión no guarda correlación con ningún factor pronóstico Conclusiones: El SXR y el MDR1 se expresan tanto en vejiga normal y tumoral. Y que dicha expresión guarda una correlación con factores pronósticos con influencia en la supervivencia descritas en la literatura. Resumen en inglés Introduction: Steroid and Xenobiotic Receptor (SXR) has demonstrated its activation by numerous drugs, including cytochrome P450 potent inducers like rifampicina or cotrimazol. The role of SXR is well known, and lies regulating in a positive manner cytochrome P450 3A4 (CYP3A4) transcription and the multidrug resistance gene (MDR1), it’s considered a key in the xenobiotic detoxification mechanism, being involved in all phases of the detoxification process. Enzymes involve (mas) d in Policyclic Aromatic hidrocarbures (PAH) metabolism and degradation are polymorphic in humans, including glutation S-transferases (GSTs), N-acetiltransferases (NATs), sulfotransferases (SULTs)1A1 and cytochrome p450 (CYP)1B1. Objectives: The objectives we’ve planned are: 1. Analyze the expression of the transcription factor SXR and MDR1 in bladder by means of RT-PCR real time, both in normal bladder and in tumoral bladder. 2. Analyze the relation between clinical and pathological factors with the expression of SXR and MDR1. 3. Analyze the expression of the polymorphims CYP1B1, GSTM1 GSTT1 and SULT1A1 and their correlation with different clinic-pathological and molecular factors. Material and Methods: In a prospective way the size of the sample was estimated. In 67 patients from two institutions (Hospital Universitario Miguel Servet (49 HUMS) and Clinica Universitaria de Navarra (18 CUN)), diagnosed of invasive bladder cancer and treated by means of radical cystectomy, were determined the expression of both SXR and MDR1 by means of real time PCR, as well as the polymorphisms CYP1B1, GSTM1 GSTT1 y SULT1A1 by means of RFLP (Restriction fragment length polymorphism). Correlations with other prognostic factors by contingency tables were performed. Results: Average follow up was 23,7 months with a median of 28,26 months. Of the 67 patients studied, 31 patients (46,3) presented disease progression, in form of local recurrence or in distant metastasis or both. With a average time to progression of 12,4 months and a median of 10 months, with a range of 1,1 month to 31,9 month. 36 patients (53,7%) did not have any evidence of disease progression during follow up. The Steroid and Xenobiotic Receptor as well as the Multidrug Resistance Gene (MDR1) are expressed in both normal bladder (0,94ΔCt y 0,94ΔCt) and tumoral bladder in the cystectomy specimen(1,09 ΔCt y 0,45 ΔCt). We’ve analyzed their expression in a quantitative manner and in a qualitative manner. The expression of SXR correlates with the presence of ca. in situ (p=0,024), vasculo-lymphatic invasion (p=0,05) mean while MDR1 correlates with presence of vasculo-lymphatic invasion (p=0,05) Both factors are correlate between each others (p=0,011). Polymorphisms: CYP1B1, GSTM1, GSTT1 and SULT1A1, are expressed in these patients but their expression doesn’t correlates with any prognostic factor. Conclusions: Both SXR and MDR1 are expressed in normal bladder as well as in tumoral bladder. And their expression correlates with different prognostic factors with influence in the survival described in the literature.

Scientific Electronic Library Online (Spanish)

2

Zea mays L. transglutaminase expression in Escherichia coli

Carvajal, Patricia; Villalobos, Enrique; Campos, Alexandre; Torné, J. Mª.; Barberà, Eduard; Santos, Mireya
2006-10-10

Digital.CSIC (Spain)

3

Xenobiotic metabolism markers in marine fish with different trophic strategies and their relationship to ecological variables

Solé, Montserrat; Rodríguez, S.; Papiol, Vanesa; Maynou, Francesc; Cartes, Joan Enric
2009-01-01

Digital.CSIC (Spain)

6
7

The transcription factor early growth response factor-1 (EGR-1) promotes apoptosis of neuroblastoma cells

Pignatelli, Miguel; Luna Medina, Rosario de; Pérez-rendón, Arturo; Santos, Ángel; Pérez Castillo, Ana
2003-08-01

Digital.CSIC (Spain)

10

Sublethal toxicity of the Prestige oil spill on yellow-legged gulls

Alonso-Álvarez, Carlos; Munilla, Ignacio; López-Alonso, Marta; Velando, Alberto
2007-08-01

Digital.CSIC (Spain)

11

Sublethal responses of the common mussel (Mytilus galloprovincialis) exposed to sodium hypochlorite and Mexel®432 used as antifoulants

López-Galindo, Cristina; Vargas-Chacoff, Luis; Nebot, Enrique; Casanueva, José F.; Rubio, Daniel; Mancera, Juan M.; Solé, Montserrat
2010-07-01

Digital.CSIC (Spain)

12

Stress biomarkers in juvenile Senegal Sole, Solea senegalensis, exposed to the water-accommodated fraction of the "Prestige" fuel oil

Solé, Montserrat; Lima, Daniela; Reis-Henriques, Maria Armanda; Santos, Miguel Machado
2008-01-01

Digital.CSIC (Spain)

13

Statins Inhibit HIV-1 infection by down-regulating rho activity

Real, Gustavo del; Jiménez Baranda, Sonia; Mira, Emilia; Lacalle, Rosa Ana; Lucas, Pilar; Gómez-Moutón, Concepción; Alegret, Marta; Peña, José María; Rodríguez Zapata, Manuel; Álvarez-Mon, Melchor; Martínez-Alonso, Carlos; Mañes, Santos
2004-08-01

Digital.CSIC (Spain)

15

Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

Kocan, Katherine M.; Zivkovic, Zorica; Blouin, Edmour F.; Naranjo, María Victoria; Almazán, Consuelo; Mitra, Ruchira; Fuente García, José de la
2009-07-16

Digital.CSIC (Spain)

18

Rescue of the streptomycin antibiotic activity by using streptidine as a "decoy acceptor" for the aminoglycoside-inactivating enzyme adenyl transferase

Latorre, Montserrat; Penalver, Pablo; Revuelta, Julia; Asensio, Juan Luis; García-Junceda, Eduardo; Bastida, Ágatha
2007-01-01

Digital.CSIC (Spain)

19

Recombinant production of serine hydroxymethyl transferase from Streptococcus thermophilus and its preliminary evaluation as a biocatalyst

Vidal, Luis; Calveras Ibáñez, Jordi; Clapés Saborit, Pere; Ferrer, Pau; Caminal, Glòria
2005-02-23

Digital.CSIC (Spain)

20

Recent insights into antioxidant defenses of legume root nodules

Becana Ausejo, Manuel; Matamoros Galindo, Manuel Antonio; Udvardi, Michael; Dalton, David A.
2010-10-01

Digital.CSIC (Spain)

21

Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus

Ghazi, Iraj; Fernández-Arrojo, Lucía; García-Arellano, Humberto; Ferrer, Manuel; Ballesteros, Antonio; Plou Gasca, Francisco José
2007-01-01

Digital.CSIC (Spain)

22

Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry

Cortón, Marta; Botella-Carretero, José I.; López, Juan A.; Camafeita, Emilio; San Millán, José L.; Escobar-Morreale, Héctor F.; Peral, Belén
2007-03-01

Digital.CSIC (Spain)

24

Pharmacogenetic profile of xenobiotic enzyme metabolism in survivors of the Spanish toxic oil syndrome

Ladona, Margarita G.; Izquierdo Martínez, Maravillas; Paz, Manuel de la; Torre, Rafael de la; Ampurdanés, Coral; Segura, Jordi; Sanz, Emilio J.
2001-03-16

Digital.CSIC (Spain)

25

Overexpression of human DNA polymerase µ (Pol µ) in a Burkitt's lymphoma cell line affects the somatic hypermutation rate

Ruiz, José F.; Lucas, Daniel; García-Palomero, Esther; Saez, Ana I.; González, Manuel A.; Piris, Miguel A.; Bernad, Antonio; Blanco, Luis
2004-11-01

Digital.CSIC (Spain)

26

On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics

Fernández Arrojo, Lucía; Alvaro, M.; Ghazi, Iraj; Abreu Felipe, Miguel de; Linde López, Dolores; Gutiérrez Alonso, Patricia; Alcalde Galeote, Miguel; Jiménez-Barbero, Jesús; Jiménez, Antonio; Ballesteros Olmo, Antonio; Fernández Lobato, María; Plou Gasca, Francisco José
2007-01-01

Digital.CSIC (Spain)

27
29

Mitochondrial glutathione, a key survival antioxidant

Marí, Montserrat; Morales, Albert; Colell, Anna; García Ruiz, Carmen; Fernández Checa, José C.
2009-11-01

Digital.CSIC (Spain)

31

Is there any detrimental effect when a chestnut hydrolysable tannin extract is included in the diet of finishing lambs?

Frutos, Pilar; Raso, Miguel; Hervás, Gonzalo; Mantecón, Ángel R.; Pérez, V.; Giráldez, Francisco Javier
2004-01-01

Digital.CSIC (Spain)

32

Interaction of tributyltin with hepatic cytochrome P450 and uridine diphosphate-glucuronosyl transferase systems of fish: in vitro studies

Morcillo, Yolanda; Janer, Gemma; O'Hara, Sean C.M.; Livingstone, David R.; Porte Visa, Cinta
2004-04-01

Digital.CSIC (Spain)

33

Influence of glutathione S-transferase polymorphisms on cognitive functioning effects induced by p,p'-DDT among preschoolers

Morales, Eva; Sunyer, Jordi; Castro-Giner, Francesc; Estivil, Xavier; Júlvez, Jordi; Ribas-Fitó, Núria; Torrent, Maties; Grimalt, Joan O.; Cid, Rafael de
2008-07-30

Digital.CSIC (Spain)

35

Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides

Ghazi, Iraj; Gómez de Segura, María Aránzazu; Fernández-Arrojo, Lucía; Alcalde Galeote, Miguel; Yates Buxcey, Malcolm; Rojas-Cervantes, M. Luisa; Plou Gasca, Francisco José; Ballesteros, Antonio
2005-06-01

Digital.CSIC (Spain)

37

Genomic organization, chromosomal localization, alternative splicing,and isoforms of the human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion processes

Lazo, Pedro A.; Nadal, Marga; Ferrer, Milagros; Area, Estela; Hernández-Torres, Javier; Nabokina, S. M.; Mollinedo, Faustino; Estivill, Xavier
2001-03-06

Digital.CSIC (Spain)

38

Función renal en cirugía cardíaca con circulación extracorpórea: Pacientes valvulares y coronarios/ Renal function during cardiac surgery with extracorporeal circulation

Lema, Guillermo; Canessa, Roberto; Urzúa, Jorge; Jalil, Roberto; Morán, Sergio; Carvajal, Claudia; Aeschlimann, Nicolás; Merino, Waldo; Jaque, María P
2008-04-01

Resumen en inglés Background: Patients with valvular heart disease are at high risk of acute renal failure after surgery with extracorporeal circulation. Aim: To describe changes in renal function parameters during surgery with extracorporeal circulation in patients with valvular heart disease and compare them with those found in patients undergoing elective coronary surgery Material and Methods: Two groups of patients were studied. Group 1 was composed by twelve patients undergoing electi (mas) ve coronary surgery and group 2 was composed by eleven patients undergoing surgery for heart valve replacement. Glomerular filtration rate and effective renal plasma now were estimated from inulin and the 131 I-hippuran clearance respectively, at five different times, during surgery and the postoperative period. Sodium filtration fraction and fractional excretion were calculated. Alpha and pi-glutathione s-transferase in urine were measured as markers of tubular damage in the pre and postoperative periods. Results: Effective renal plasma flow was reduced in both groups before induction of anesthesia, did not change during surgery and decreased significantly in patients with valvular disease in the postoperative period. Glomerular filtration rates were normal during all the study period. There was a non significant reduction of filtration fraction during extracorporeal circulation. Alpha and pi glutathione s-transferases were normal and did not change. Fractional excretion of sodium increased significantly postoperatively Conclusions: In patients with valvular disease undergoing surgery with extracorporeal circulation, renal function does not deteriorate. No significant difference was found when compared with patients undergoing coronary surgery. No evidence of functional and cellular renal disfunction or damage was found in both study groups

Scientific Electronic Library Online (Spanish)

39

First evidence of polybrominated diphenyl ether (flame retardants) effects in feral barbel from the Ebro River basin (NE, Spain)

Raldúa, Demetrio; Padrós, Francesc; Solé, Montserrat; Eljarrat, Ethel; Barceló, Damià; Riva, M. Carmen; Barata Martí, Carlos
2008-08-01

Digital.CSIC (Spain)

41

Epigenética en asma/ Epigenetics in asthma

Vergara Rivera, Candelaria; Sánchez Caraballo, Jorge Mario; Martínez Alfaro, Beatriz; Caraballo Gracia, Luis
2009-12-01

Resumen en español El asma es una enfermedad respiratoria crónica con alta heredabilidad. Se ha propuesto que en su patogénesis participan varios genes con efectos variables al igual que factores ambientales, y se ha sugerido que los mecanismos epigenéticos pueden mediar parte del efecto de los factores ambientales en el comienzo y la evolución de la enfermedad. La epigenética describe los cambios en la expresión génica heredables durante las mitosis y meiosis que no son codificados (mas) en la secuencia de ADN. Ellos incluyen la metilación o desmetilación del ADN y la acetilación, desacetilación, ubiquitinación, sumoilación y fosforilación de histonas, cambios en los microARN y alteraciones cromatínicas. En esta revisión se describen hallazgos que establecen una relación entre algunos mecanismos epigenéticos y el proceso inflamatorio y la exposición a factores ambientales en el asma. Ellos incluyen: el aumento en la actividad de las acetilasas de histonas y de la expresión de las enzimas acetiladoras; la disminución de las enzimas desacetiladoras en los pulmones de individuos asmáticos; el aumento de la expresión del factor nuclear NF-?B durante el proceso inflamatorio alérgico; cambios en la metilación/desmetilación del ADN durante la diferenciación de los linfocitos y la estimulación/supresión de genes como los de la IL-4 y el IFN-?, respectivamente. El humo del cigarrillo, las infecciones bacterianas y virales, la dieta materna y la polución ambiental son otros factores que desencadenan procesos epigenéticos como la acetilación de histonas, la inducción de citoquinas inflamatorias, la inactivación de las desacetilasas de histonas, la polarización de la respuesta inmune hacia el tipo Th2 y una mayor producción de IgE y citoquinas de este perfil. Se revisan también los efectos epigenéticos resultantes de la terapia con corticoides y teofilina y otros factores que podrían influir en el riesgo de asma en la infancia como la ingesta materna de frutas, legumbres, aceites de pescado, vitaminas, minerales y probióticos y el uso de antibióticos durante el embarazo. Resumen en inglés Epigenetics in asthma Asthma is a chronic respiratory disease with a high heritability. It has been postulated that several genes with variable effects are involved in its pathogenesis along with environmental factors. It has been suggested that epigenetic mechanisms can mediate the effects of environmental factors on the onset and progression of the disease. Epigenetics describes inheritable changes in gene expression inherited during meiosis and mitosis that are not enc (mas) oded in the DNA sequence. They include DNA methylation/ demethylation, acetylation, deacetylation, ubiquitination, SUMOylation and phosphorylation of histones, changes in microARN and alterations of chromatine. In this article we review some findings that establish a relationship between some epigenetic mechanisms and the inflammatory process in asthma and exposure to environmental factors. They include increasing the activity of histone acetyl-transferases and the expression of histone acetylating enzymes, decrease of deacetylating enzymes in the lungs of asthmatics; increased expression of the transcription factor NF-?B in the allergic inflammatory process, changes in methylation/demethlylation of DNA during the differentiation of lymphocytes and the stimulation/ suppression of IL-4 and IFN? genes, respectively. Smoking, bacterial and viral infections, maternal diet and environmental pollution are also factors that trigger epigenetic processes such as histone acetylation and induction of inflammatory cytokines, inactivation of histone deacetytransferases, polarization of the immune response toward the Th2 type and increased production of IgE and cytokines of this profile. We review the epigenetic effects resulting from therapy with corticosteroids and theophylline, and other factors that might influence the risk of asthma in childhood such as maternal intake of fruits, vegetables, fish oils, vitamins, minerals, and the use of probiotics and antibiotics during pregnancy.

Scientific Electronic Library Online (Spanish)

42

Effects on feeding rate and biomarker responses of marine mussels experimentally exposed to propranolol and acetaminophen

Solé, Montserrat; Shaw, Jennifer P.; Frickers, Patricia E.; Readman, James W.; Hutchinson, Thomas H.
2009-10-18

Digital.CSIC (Spain)

43

Effects of novel maturity-onset diabetes of the young (MODY)-associated mutations on glucokinase activity and protein stability

Galán, María; Vincent, Olivier; Roncero, Isabel; Azriel, Sharona; Boix-Pallares, Pedro; Delgado-Álvarez, Elías; Díaz-Cadórniga, Fernando; Blázquez, Enrique; Navas, M. Ángeles
2006-01-01

Digital.CSIC (Spain)

44

Effects of 17β-estradiol and 4-nonylphenol on osmoregulation and hepatic enzymes in gilthead sea bream (Sparus auratus)

Pérez Carrera, Erkuden; García-López, Angel; Martín del Río, María del Pilar; Martínez-Rodríguez, Gonzalo; Solé, Montserrat; Mancera, Juan M.
2007-01-01

Digital.CSIC (Spain)

45

Effect of zinc supplementation on antioxidant status in rats fasciolosis

Gabrashanska, M.; Tepavitcharova, S.; Manga-González, M. Yolanda; Ermidou-Pollet, S.; Mizinska-Boevska, Y.; Miñambres Rodríguez, Baltasar; Anisimova, M.
2007-01-01

Digital.CSIC (Spain)

46

Effect of microbial transglutaminase on the protein fractions of rice, pea and their blends

Marco, Cristina; Pérez, Gabriela T.; Ribotta, Pablo D.; Rosell, Cristina M.
2007-11-01

Digital.CSIC (Spain)

48

Combined use of biomarkers and in situ bioassays in Daphnia magna to monitor environmental hazards of pesticides in the field

Barata Martí, Carlos; Damásio, Joana B.; López, Miguel Ángel; Kuster, Marina; López de Alda, Miren; Barceló, Damià; Riva, Mari Carmen; Raldúa, Demetrio
2007-02-01

Digital.CSIC (Spain)

52

Biochemical mechanisms of resistance in Daphnia magna exposed to the insecticide fenitrothion

Damásio, Joana B.; Guilhermino, Lúcia; Soares, Amadeu M. V. M.; Riva, M. Carmen; Barata Martí, Carlos
2007-08-30

Digital.CSIC (Spain)

53

Bioaccumulation and biochemical responses in mussels exposed to the water-accommodated fraction of the Prestige fuel oil

Solé, Montserrat; Buet, Astrid; Ortiz, Laura; Maynou, Francesc; Bayona Termens, Josep María; Albaigés Riera, Joan
2007-01-01

Digital.CSIC (Spain)

54

Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides

Ghazi, Iraj; Fernández-Arrojo, Lucía; Gómez de Segura, María Aránzazu; Alcalde Galeote, Miguel; Plou Gasca, Francisco José; Ballesteros, Antonio
2009-01-01

Digital.CSIC (Spain)

57

A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis

Juárez, Raquel; Ruiz, José F.; Nick McElhinny, Stephanie A.; Ramsden, Dale; Blanco, Luis
2006-09-08

Digital.CSIC (Spain)