Sample records for TABACO (PLANTA) (tobacco plant)
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1

IDENTIFICACIÓN DEL AGENTE CAUSAL DE UNA BACTERIOSIS EN ÑAME (Dioscorea alata L.)/ ISOLATION AND IDENTIFICATION OF A PHYTOPATHOGENIC BACTERIA FROM WATER YAM (Dioscorea alata L.)/ IDENTIFICAÇÃO DO AGENTE CAUSAL DE UMA BACTERIOSE NO INHAME (Dioscorea alata L.)

Rodríguez, Margarita; Matehus, Juan; Gerstl, Armando; Santana, María A.
2008-07-01

Resumen en portugués O inhame (Dioscorea alata L.), amplamente usado como fonte de calorias na África, Ásia e no Caribe, é uma planta herbácea e trepadeira que se reproduz geralmente de forma vegetativa e produz um tubérculo comestível de grande tamanho com alto conteúdo de amido. Vários microorganismos fitopatógenos, incluindo vírus, fungos e bactérias, afetam ao cultivo. Plantas enfermas da zona de Guarataro, estado Bolívar, Venezuela, foram coletadas em 2003, ano em que houve ~ (mas) 80% de perdas na produção de inhame dessa zona a causa de uma enfermidade desconhecida. Folhas coletadas de plantas enfermas com lesões aquosas e necróticas foram esterilizadas superficialmente e transferidas a uma solução salina para permitir a difusão de bactérias desde as bordas do tecido e ser logo plantadas em meio LBA. Como controles foram empregados água e meio LBA. Colônias amarelas, brilhantes e lisas, foram visíveis às 24-48h de incubação a 30ºC. Vários isolados foram inoculados nas provas de hipersensibilidade em tabaco e de patogenicidade em plântulas de inhame cultivadas in vitro. Vários graus de resposta de hipersensibilidade em tabaco foram observados. As de plântulas de inhame inoculadas mostraram lesões aquosas na lâmina foliar, as quais não se observaram nos controles. Isolados bacterianos foram observados mediante microscopia de luz, revelando bactérias bacilares Gram negativas. Vários flagelos perítricos foram observados por microscopia eletrônica. Os resultados das provas bioquímicas e fisiológicas indicaram que os isolados correspondem a Pantoea agglomerans (Enterobacteriacea). Discutem-se diferentes metodologias para a identificação de Resumen en español El ñame (Dioscorea alata L.), ampliamente usado como fuente de calorías en África, Asia y el Caribe, es una planta herbácea y trepadora que se reproduce generalmente de forma vegetativa y produce un tubérculo comestible de gran tamaño con alto contenido de almidón. Varios microorganismos fitopatógenos, incluyendo virus, hongos y bacterias, afectan al cultivo. Plantas enfermas de la zona de Guarataro, estado Bolívar, Venezuela, fueron colectadas en el 2003, año e (mas) n que hubo ~80% de pérdidas en la producción de ñame de la zona a causa de una enfermedad desconocida. Hojas colectadas de plantas enfermas con lesiones acuosas y necróticas fueron esterilizadas superficialmente y transferidas a solución salina para permitir la difusión de bacterias desde los bordes del tejido y ser luego sembradas en medio LB agar. Como controles se emplearon agua y medio LB. Colonias amarillas, brillantes y lisas, fueron visibles a las 24-48h de incubación a 30ºC. Varios aislados fueron inoculados en las pruebas de hipersensibilidad en tabaco y de patogenicidad en plántulas de ñame cultivadas in vitro. Varios grados de respuesta de hipersensibilidad en tabaco fueron observados. Las plántulas de ñame inoculadas mostraron lesiones acuosas en la lámina foliar, las cuales no se observaron en los controles. Aislados bacterianos fueron observados mediante microscopía de luz, revelando bacterias bacilares Gram negativas. Varios flagelos peritricos fueron observados por microscopía electrónica. Los resultados de las pruebas bioquímicas y fisiológicas indicaron que los aislados corresponden a Pantoea agglomerans (Enterobacteriacea). Se discuten diferentes metodologías para la identificación de bacterias. Resumen en inglés Water yam (Dioscorea alata L.), a staple crop in Africa, Asia and the Caribbean, is a herbaceous twining vine plant generally reproduced by vegetative sections and highly affected by several phytopathogenic organisms including virus, fungi and bacteria. Diseased plants were collected from Guarataro, Bolívar State, Venezuela, in 2003. That year an unknown disease led to ~80% loss of water yam production. The leaves from diseased plants have watery or necrotic lesions on t (mas) he leaf lamina. Pieces of necrotic tissue were surface sterilized, then transferred to saline solution and the bacteria allowed to diffuse into the solution from the edge of the dissected tissue. The bacteria were isolated on LB agar media. Yellow, shining, smooth colonies with regular margins were visible within 24-48h of incubation at 30ºC. Several isolates were inoculated on tobacco plants. Water and LB media were used as negative controls. Several degrees of hypersensitivity response resulted from the inoculation of tobacco. However, inoculation of in vitro water yam plants showed watering lesions on the leaf lamina inoculated with bacteria cultures and no lesions on water and media inoculated leaves. Bacterial cells observed by light microscopy showed Gram-negative regular rods. Several peritricous flagella were observed under EM. Data from biochemical and physiological tests indicated that the isolates belong to the Enterobacteriaceas family specifically to Pantoea agglomerans. Different methodologies for bacterial identification are discussed.

Scientific Electronic Library Online (Spanish)

2

Nicotiana glauca L. Arvense es Reservorio de Virus Fitopatógenos/ Nicotiana glauca L. is a reservoir weed of plant viruses

Aviña-Padilla, Katia; Ochoa-Sánchez, Juan Carlos; Martínez-Soriano, Juan Pablo
2008-01-01

Resumen en español Existe una gran diversidad de factores involucrados en el inicio y desarrollo de las epifitias virales, por lo que el estudio de cada uno de ellos representa una gran complejidad. Un factor importante en la ecología de cualquier enfermedad es la existencia de hospedantes que en muchas ocasiones son malezas presentes dentro de los cultivos o en zonas aledañas y que pueden ser reservorios de patógenos y sus vectores. Nicotiana glauca es una solanácea perenne de amplia d (mas) istribución en México en la cual es común observar síntomas de virosis. Dada la importancia de esta planta como posible reservorio de virus importantes en plantas cultivadas, se realizó este estudio enfocado a determinar la etiología de los síntomas que muestra en campo. Se colectaron muestras de N. glauca con síntomas típicos de virosis en puntos estratégicos del Centro de la República Mexicana. Se detectaron los siguientes virus: Virus Mosaico del Pepino (VMP), Virus Mosaico del Tabaco (VMT) y Virus Y de la Papa (VYP); la presencia de mezclas virales no fue un fenómeno común. Se obtuvieron dieciséis aislamientos del VMT, nueve del VMP y tres de VYP. No se detectó presencia de geminivirus o viroides. Resumen en inglés A great number of factors are involved in the beginning and further development of plant viral epiphytotics, thus the study of each one is of great complexity. An important factor in the ecology of any disease is the presence of hosts which in many instances are weeds present within the crop or in adyacent areas and that may harbor pathogens or their vectors. Nicotiana glauca is a perennial solanaceae plant of wide distribution in Mexico in which it is common to observe v (mas) iral symptoms. Given the importance of this host as possible reservoir of important viruses of cultivated plants, our study focused to determine the etiology of the symptoms observed in the field. N. glauca samples with viral symptoms were collected from strategic points of the central part of Mexico. The following viruses were detected: Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Potato virus Y (PVY); the presence of viral mixtures was not a common phenomenon. Sixteen isolations of TMV were obtained, nine of CMV and three of PVY. No geminiviruses or viroids were detected.

Scientific Electronic Library Online (Spanish)

3

EXPERIENCIAS PARA EL MANEJO INTEGRADO DE CERCOSPORA NICOTIANAE ELLIS & EVERH EN TABACO NEGRO, LOCALIDAD DE JUAN GUERRA. TARAPOTO. PERÚ/ EXPERIENCES FOR THE MANAGEMENT OF CERCOSPORA NICOTIANAE ELLIS & EVERH IN BLACK TOBACCO-TOWN OF JUAN GUERRA, TARAPOTO-PERU

Julca Otiniano, Alberto; Julca Vera, Noemí; Blas-Sevillano, Raúl; Bello Amez, Segundo; Carhuallanqui Pérez, Ruth; Crespo Costa, Reynaldo
2008-04-01

Resumen en español En la localidad de Juan Guerra (Tarapoto, Perú) se realizaron diversos estudios que permitieran diseñar en el futuro un programa de manejo integrado de la “Cercosporiosis” causada por Cercospora nicotianae en tabaco negro para puros (habanos). Se determinó que la severidad y la incidencia de la enfermedad están altamente correlacionadas. La severidad aumentó con la edad de la planta de tabaco y de las dos variedades evaluadas la HN2 fue la que presentó l (mas) a menor severidad. La “Cercosporiosis” fue más importante en la época lluviosa, época en la que se registró la mayor severidad. También se encontró que la densidad de plantación no afectó significativamente la severidad de la enfermedad y tampoco hubo efecto significativo de los niveles de fertilización mineral estudiados. La severidad fue significativamente menor cuando el control de brotes axilares se realizó manualmente y la aplicación de fungicidas controló significativamente la enfermedad. Los mejores resultados estuvieron asociados con la mezcla de fungicidas de contacto y sistémicos Resumen en inglés It was determined that the severity and the incidence of the disease are highly correlated. The severity, increased with the age of the tobacco plant. When comparing the two evaluated varieties the HN2 was the one that showed the less severity. The “Cercosporiosis” was more important in the rainy season in which the greatest severity was recorded. It was also found that the plantation density didn’t affect significantly the severity of the disease. Neith (mas) er there was a significant effect of the mineral fertilization levels under study. The severity was significantly smaller when the control of growth of axillary buds was carried out manually and the application of fungicides controlled the disease significantly. The best results were associated with the mixture of contact and systemic fungicides

Scientific Electronic Library Online (Spanish)

4

Evaluación agronómica de accesiones de Capsicum del banco de germoplasma de la Universidad Nacional de Colombia Sede Palmira/ Agronomic evaluation of Capsicum accessions of the gene Bank of the National University of Colombia at Palmira's campus

Pardey Rodríguez, Catherine; García Dávila, Mario Augusto; Vallejo Cabrera, Franco Alirio
2009-01-01

Resumen en español En un diseño de bloques incompletos 10 x 10 con tres repeticiones se estudiaron descriptores cuantitativos relacionados con producción por planta, contenido de capsaicina y presencia de virus en 100 accesiones de Capsicum pertenecientes a la Universidad Nacional de Colombia. Las diferencias entre las accesiones fueron explicadas por el contenido de capsaicina, características asociadas con la producción por planta y características del fruto. Se encontró correlació (mas) n inversa entre el contenido de capsaicina y producción; número de frutos por planta con peso de fruto. La dispersión de las accesiones en el plano cartesiano situó las variedades comerciales Cayenne, Tabasco y Habanero, en función de producción, peso y cantidad de frutos, en posiciones desventajosas con respecto a los promedios generales de las características de las otras accesiones. Cuatro accesiones superaron a Tabasco en contenido de capsaicina. Cuatro tipos de virus (potyvirus, geminivirus y cucumovirus y virus del mosaico del tabaco TMV) se presentaron en el lote de evaluación. Los virus actuaron en forma conjunta; los potyvirus con los cucumovirus y TMV; los geminivirus con TMV. Resumen en inglés 100 accessions from Capsicum gene Bank of the National University of Colombia at Palmira's campus, under field conditions of the experimental station at Candelaria, Cauca Valley were studied. In a design of incomplete blocks 10 x 10 with three repetitions, quantitative descriptions were evaluated. The characteristics were related with yield, capsaicin content and virus presence.The agronomic evaluation found that the differences among the accessions are explained for (mas) the capsaicin content, yield, characteristics associated and fruits characteristics associated. Inverse correlation among the capsaicin content with production, quantity of fruits for plant with weight was found. The dispersion of the accessions in the Cartesian axis placed the commercial varieties: Cayenne, Tabasco and Habanero in terms of yield, weight and number of fruits in unfavorable positions with regard to the general averages. The same was observed for the stability of the characteristics above mentioned. There were four introductions with high capsaicin contents that overcome Tabasco. Four virus types affecting plants were evaluated: potyvirus, geminivirus, cucumovirus and virus of the mosaic of the tobacco (TMV). The viruses were acting together; the potyvirus with the cucumovirus and TMV and geminivirus with TMV.

Scientific Electronic Library Online (Spanish)

5

Estudio comparativo sobre dos métodos para determinar el potencial hídrico en el cultivo del guayabo (Psidium guajava L.) en la Planicie de Maracaibo/ A comparative study on two methodologies for determining water potential in guava trees (Psidium guajava L.) on the Maracaibo Plain

Urdaneta, T; Araujo, F. J.; Lugo, L
2003-01-01

Resumen en español Para definir la técnica de medición de potencial hídrico en el cultivo del guayabo (Psidium guajava L.) se llevó a cabo una investigación en la finca "Los Cienegos" del estado Zulia. Se seleccionaron 8 plantas de guayabo sembradas a pié franco y 8 plantas injertadas de 3 años de edad, sembradas a distancia de 5 m x 5 m, con manejo agronómico similar. Las variables estudiadas fueron el potencial hídrico del tallo (Yt) y el potencial hídrico de la hoja (Yh). Para (mas) la medición del potencial hídrico del tallo se utilizaron los criterios empleado por Begg y Turner (1970) en el cultivo del tabaco (Nicotina tabaco L.) y McCutchuam y Shackeland (1992) en el cultivo del durazno (Prunus domestica L). Para la medición del potencial hídrico de la hoja, se empleó el criterio establecido para plantas vasculares por Scholander (1965). Los resultados demostraron que existe poca diferencia entre los potenciales hídricos del tallo y de la hoja para las primeras horas de la mañana, mientras que se registraron diferencias de mayor magnitud en horas del mediodía, entre las 12 y las 14 horas. La variabilidad en los potenciales de la hoja durante este último periodo, también fue mayor que los potenciales del tallo. Después de las 14 horas, los valores de potenciales se estabilizaron y comenzó el proceso de rehidratación de la planta. Resumen en inglés This study was carried out for the purpose of defining a reliable technique for measuring water potential of guava trees (Psidium guajava L.). Eight self-rooted and eight grafted three years old trees were selected at "Los Ciénegos" farm, Zulia state, planted at a distance of 5 m x 5 m, with similar agronomic management. Leaf (yL) and stem (yS) water potentials were quantified using three different techniques. The Begg and Turner (1970) criteria for tobacco was utilized (mas) for the measurement of stem potential as well as the McCutcham and Shackeland (1992) criteria, applied to tobacco (Nicotiana tabacum L.) and peach (Prunus domestica L.), respectively. While the Scholander (1965) criteria was applied for measuring yL. Results showed that there was little difference between yS and yL early in the morning, but it greater differences occured at noon, specifically between the 12 (noon) and 14 (2PM)hours. More over, a greater variability was found in yL as compared to yS during this period. After 14 hours (2PM), water potential values became stabilized and the plant re-hydration process began.

Scientific Electronic Library Online (Spanish)

6

EMERGENCE OF BEGOMOVIRUSES IN CUBA/ EMERGENCIA DE BEGOMOVIRUS EN CUBA

Martínez, Yamila
2008-04-01

Resumen en español Los begomovirus son patógenos de plantas que en las últimas décadas han causado importantes pérdidas a cultivos de interés económico, en regiones tropicales y subtropicales. En Cuba se han identificado nueve begomovirus afectando cultivos de importancia económica como tomate, fríjol, tabaco, pimiento, calabaza, papa, y malezas de diferentes familias botánicas. En este trabajo se analizaron las secuencias de la región intergénica (RI) y la región N- Terminal de (mas) la proteína asociada a la replicación (Rep) de los diferentes begomovirus identificados en Cuba, referidas en el banco de datos de genes (Genebank), utilizando programas estadísticos para los alineamientos, análisis filogenéticos y búsqueda de recombinaciones. La detección de posibles eventos de recombinaciones fue realizada para p=0 .05, basado en 10000 permutaciones. En la región intergénica se detectaron entre 11 y 15 sitios de recombinaciones, entre el virus del mosaico amarillo del Macroptilium (MaYMV) y los virus de la hoja rugosa del tabaco (TLRV), el virus moteado taino del tomate (TMoTV) y el virus del mosaico amarillo de Dicliptera (DiYMoV). En la región N-Terminal de la Rep., se detectaron además, 16 sitios de recombinaciones entre MaYMV y el virus amarillo dorado de la Sida (SiGYVV). Los virus del mosaico de Jatropha (JMV) procedente de Jatropha gossipifolia , y los aislamientos de TYLCV-IL(CU) de tomate y pimiento, mostraron secuencias que se encuentran en todos los sitios polimórficos detectados y que son homólogas a los sitios monomórficos, de los alineamientos analizados. En la región N-Terminal de la Rep del TTMoV y DiYMoV, se detectaron 3 y 4 sitios polimórficos respectivamente, con características similares a los anteriores y constituyen evidencia de recombinaciones pasadas y destruida en la evolución. Todos los sitios identificados en estos aislamientos pueden definir características de las especies de begomovirus identificados. Resumen en inglés Begomoviruses are plant pathogens that caused important losses to crops of economic interest in tropical and subtropical regions in the last decade. In Cuba, nine begomoviruseshave been identified affecting tomato, beans, tobacco, pepper, squash, potato and weeds of different botanical families. The intergenic sequence (IR) and the N-terminal regions of the replication associated protein (Rep) of begomoviruses identified in Cuba were analysed using sequence data of gene c (mas) ollections (GeneBank). Statistical programs were used for the alignments, phylogenetic analysis and searching of significant recombination fragments. The detection of possible recombination events was carried out for p=0.05, based on 10000 permutations. Eleven IR recombination event sites between Macroptilium Yellow Mosaic Virus (MaYMV ) and Tobacco Leaf Rugose Virus (TLRV), and 15 between Tomato Mottle Taino Virus (TMoTV) and Dicliptera Yellow Mosaic Virus (DiYMoV ) were found. Sixteen recombination event sites between MaYMV and Sida Golden Yellow Vein Virus (SiGYVV ) were also found in the Rep region. Jatropha Mosaic Virus (JMV) from Jatropha gossipifolia and Tomato Yellow Leaf Curl Virus TYLCV-IL( CU) from tomato and pepper showed base sequences in the alignments that were present in all the polymorphic sites detected and homologous to monomorphic sites. TMoTV and DiYMoV showed 3 and 4 polymorphic sites in the Rep N-terminal region, respectively. All these sites constitute evidences of old recombination events destroyed during the evolution, and they may define the characteristics of the begomovirus species identified.

Scientific Electronic Library Online (Spanish)

7

Vegetative and Seed-Specific Forms of Tonoplast Intrinsic Protein in the Vacuolar Membrane of Arabidopsis thaliana

Höfte, Herman; Hubbard, Lauren; Reizer, Jonathan; Ludevid, M. Dolors; Herman, Eliot M.; Chrispeels, Maarten J.
1992-06-01

Digital.CSIC (Spain)

9

Upper-limit mutation rate estimation for a plant RNA virus

Sanjuán, Rafael; Agudelo-Romero, Patricia; Elena, Santiago F.
2009-05-12

Digital.CSIC (Spain)

10

Transgenic Tobacco Plants Overexpressing Chitinases of Fungal Origin Show Enhanced Resistance to Biotic and Abiotic Stress Agents

Dana, María de la Mercedes; Pintor-Toro, José A.; Cubero, Beatriz

Genes encoding defense-related proteins have been used to alter the resistance of plants to pathogens and other environmental challenges, but no single fungal gene overexpression has produced broad-spectrum stress resistance in transgenic lines. We have generated transgenic tobacco (Nicotiana tabacu...

DRIVER (Spanish)

13

The plastidial 2-C-methyl-D-erythritol 4-phosphate pathway provides the isoprenyl moiety for protein geranylgeranylation in tobacco BY-2 cells

Gerber, Esther; Hemmerlin, Andréa; Hartmann, Michael; Heintz, Dimitri; Hartmann, Marie-Andrée; Mutterer, Jerome; Rodríguez Concepción, Manuel; Boronat, Albert; Van Dorsselaer, Alain; Rohmer, Michel; Crowell, Dring N.; Bach, Thomas J.
2009-01-09

Digital.CSIC (Spain)

16

The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9

Almoguera, Concepción; Prieto-Dapena, P.; Díaz-Martín, J.; Espinosa, J.M.; Carranco, R.; Jordano, Juan
2009-06-19

Digital.CSIC (Spain)

17

Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants

Pardo, José M.; Reddy, Muppala P.; Yang, Shuli; Maggio, Albino; Huh, Gyung-Hye; Matsumoto, Tracie; Coca, María A.; Paino-D’Urzo, Matilde; Koiwa, Hisashi; Yun, Dae-Jin; Watad, Abed A.; Bressan, Ray A.; Hasegawa, Paul M.
1998-08-01

Digital.CSIC (Spain)

18

Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein

Yun, Dae-Jin; Zhao, Yuan; Pardo, José M.; Narasimhan, Meena L.; Damsz, Barbara; Lee, Hyeseung; Abad, Laura R.

Copyright © by National Academy of Sciences. La versión original está disponible en http://www.pnas.org/ | Strains of the yeast Saccharomyces cerevisiae differ in their sensitivities to tobacco osmotin, an antifungal protein of the PR-5 family. However, cells sensitive to tobacco osmotin showed resi...

DRIVER (Spanish)

19

Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein

Yun, Dae-Jin; Zhao, Yuan; Pardo, José M.; Narasimhan, Meena L.; Damsz, Barbara; Lee, Hyeseung; Abad, Laura; Paino-D’Urzo, Matilde; Hasegawa, Paul M.; Bressan, Ray A.
1997-06-01

Digital.CSIC (Spain)

21

Reversible Heat-Induced Inactivation of Chimeric β-Glucuronidase in Transgenic Plants

Almoguera, C.; Rojas, A.; Jordano, Juan

We compared the expression patterns in transgenic tobacco (Nicotiana tabacum) of two chimeric genes: a translational fusion to ß-glucuronidase (GUS) and a transcriptional fusion, both with the same promoter and 5'-flanking sequences of Ha hsp17.7 G4, a small heat shock protein (sHSP) gene from sunfl...

DRIVER (Spanish)

22

Repression by an auxin/indole acetic acid protein connects auxin signaling with heat shock factormediated seed longevity

Carranco, R.; Espinosa, J.M.; Prieto-Dapena, P.; Almoguera, Concepción; Jordano, Juan
2010-11-29

Digital.CSIC (Spain)

23

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.

Araya, Alexandre; Zabaleta, Eduardo; Blanc, Valérie; Bégu, Dominique; Hernould, Michel; Mouras, Armand; Litvak, Simon
1998-04-01

Resumen en inglés RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to those from organisms where this process is absent. This biochemical process involves cytidine deamination. The cytoplasmic male sterility (CMS) phenotype generated by the inco (mas) mpatibility between the nuclear and the mitochondrial genomes is an important agronomical trait which prevents inbreeding and favors hybrid production. The hypothesis that RNA editing leads to functional proteins has been proposed. This hypothesis was tested by constructing transgenic plants expressing a mitochondrial protein translated from unedited mRNA. The transgenic "unedited" protein was addressed to the mitochondria leading to the appearance of mitochondrial dysfunction and generating the male sterile phenotype in transgenic tobacco plants. Male sterile plants were also obtained by expressing specifically a bacterial ribonuclease in the anthers. The economical benefits of artificially engineered male-sterile plants or carrying the (native) spontaneous CMS phenotype, implies the restoration to obtain fertile hybrids that will be used in agriculture. Restoration to fertility of transgenic plants was obtained either by crossing male-sterile plants carrying the "unedited" mRNA with plants carrying the same RNA, but in the antisense orientation or, in the case of plants expresing the ribonuclease, by crossing male-sterile plants with plants expressing an inhibitor specific of this enzyme

Scientific Electronic Library Online (Spanish)

26

Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance

Verberne, Marianne C.; Verpoorte, Rob; Bol, John F.; Mercado-Blanco, Jesús; Linthorst, Huub J.M.
2000-07-01

Digital.CSIC (Spain)

27

Novel HSF transcripcion factors and use thereof in trangenic plants.

Almoguera, Concepción; Rojas, A.; Díaz Martín, Juan Antonio; Prieto-Dapena, P.; Carrasco Galán, Raúl; Jordano, Juan
2004-01-22

Digital.CSIC (Spain)

28

Métodos de control de las principales plagas, enfermedades y malezas en Calendula officinalis L./ Method of control of the main pests, diseases and underbrush in Calendula officinalis L.

Rivera Amita, María Magdalena; Milanés Figueredo, Masgloiris; Ramos Gálvez, Silvino Raúl
2005-04-01

Resumen en español En las condiciones de Cuba la especie Calendula officinalis L. es afectada por insectos y malezas, también diferentes hongos en sus hojas y flores le producen enfermedades. Para el control de las plagas y enfermedades, se emplearon 2 plaguicidas de origen vegetal: tabaquina, subproducto del tabaco (Nicotiana tabacum L.) y semillas secas maceradas de paraíso (Melia azederach L.) extraídos por repercolación con etanol al 70 % y un producto de origen biológico obtenido (mas) de las mezclas de los hongos Beauveria bassiana, Verticillum lecanii y Trichoderma viridis con la bacteria Bacillus thuringiensis. Cada tratamiento se replicó 3 veces e incluyó un testigo. Para el control de las malezas se empleó solamente el método cultural mediante escarde y guataquea. Los métodos utilizados para el control de las plagas que más daños causan a esta especie y de las enfermedades, resultaron ser efectivos excepto para el control de los Trips que mostró resistencia a todos los productos probados, lo que pudo estar dado por la colindancia entre los cultivos. Resumen en inglés In the Cuban conditions, the Calendula officinalis L. species is affected by insects and underbrush. Different fungi in its leaves and flowers also produce diseases. In order to control pests and diseases, 2 pesticides of plant origin were used: tabaquina, a by-product of tobacco (Nicotiana tabacum L) and dry macerated seeds of chinaberry (Melia azederach L.) extracted by repercolation with ethanol 70 % and a product of biological origin obtained from the mixture of Beauv (mas) eria bassiana, Verticillum lecanii and Trichoderma viridis fungi with the Bacillus thuringiensis bacterium. Each treatment was replicated 3 times and included a control. To control underbrush, it was only used the cultivation method by weeding and hoeing. The methods used for the control of the most harmful pests and diseases proved to be effective, excepting the control of Trips that were resistant to all the tested products, which may result from the contiguity between cultivations.

Scientific Electronic Library Online (Spanish)

29

Molecular and functional analysis of α-amylase inhibitor genes and proteins in the common bean Phaseolus vulgaris

Mirkov, T. E.; Pueyo, José J.; Mayer, J. E.; Kjemtrup, S.; Cardona, C.; Chrispeels, Maarten J.
1994-01-01

Digital.CSIC (Spain)

30

Molecular analysis of a phylogenetically conserved carrot gene: developmental and environmental regulation

William, S. Seffens; Almoguera, Concepción; Wilde, H.D.; Von der Haar, Raymond A.; Thomas, T.L.
1990-01-01

Digital.CSIC (Spain)

31

Loss of function of the HSFA9 seed longevity program

Tejedor-Cano, J.; Prieto-Dapena, P.; Almoguera, Concepción; Carranco, R.; Hiratsu, K; Ohme-Takagi, M.; Jordano, Juan
2010-04-22

Digital.CSIC (Spain)

32

Induction of the Hahsp17.7G4 promoter by root-knot nematodes: involvement of heat-shock elements in promoter activity in giant cells

Escobar, Carlolina; Barcala, Marta; Portillo, Mary; Almoguera, Concepción; Jordano, Juan; Fenoll, Carmen
2003-12-01

Digital.CSIC (Spain)

33

Improved resistance to controlled deterioration in transgenic seeds.

Prieto-Dapena, P.; Castaño, Raúl; Almoguera, Concepción; Jordano, Juan
2006-09-22

Digital.CSIC (Spain)

34

Implication of the C terminus of the Prunus necrotic ringspot virus movement

Aparicio Herrero, Frederic; Pallás, Vicente; Sánchez-Navarro, J. A.
2010-03-10

Digital.CSIC (Spain)

35

Host-parasite relationships in fall-sown sugar beets infected by the stem and bulb nematode, Ditylenchus dipsaci

Castillo, Pablo; Vovlas, Nicola; Azpilicueta, A.; Landa, Blanca B.; Jiménez-Díaz, Rafael M.
2007-01-01

Digital.CSIC (Spain)

37

Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens

Lorito, Matteo; Woo, Sheridan L.; Garcia Fernandez, Irene; Colucci, Gabriella; Harman, Gary E.; Pintor-Toro, José A.; Filippone, Edgardo; Muccifora, Simona; Lawrence, Christopher B.; Zoina, Astolfo; Tuzun, Sadik; Scala, Felice
1998-07-07

Digital.CSIC (Spain)

38

Eukaryotic protein production in designed storage organelles

Torrent, Margarita; Llompart, Blanca; Lasserre-Ramassamy, Sabine; Llop-Tous, Immaculada; Bastida, Miriam; Marzabal, Pau; Westerholm-Pavinen, Ann; Saloheimo, Markku; Heifetz, Peter B.; Ludevid, M. Dolors
2009-01-28

Digital.CSIC (Spain)

43

Changes in the gene expression profile of Arabidopsis thaliana after infection with Tobacco etch virus

Agudelo-Romero, Patricia; Carbonell, Pablo; de la Iglesia, Francisca; Carrera, Javier; Rodrigo, Guillermo; Jaramillo, Alfonso; Pérez Amador, Miguel A.; Elena, Santiago F.
2008-08-07

Digital.CSIC (Spain)

44

Cecropin A-derived peptides are potent inhibitors of fungal plant pathogens

Cavallarín, Laura; Andreu, David; San Segundo, Blanca
1998-03-01

Digital.CSIC (Spain)

46

An Imperfect Heat Shock Element and Different Upstream Sequences Are Required for the Seed-Specific Expression of a Small Heat Shock Protein Gene

Carranco, R.; Almoguera, C.; Jordano, Juan

La editorial de la revista ha restringido el acceso, consulte el artículo en la url de la publicación si está suscrito: http://www.plantphysiol.org/ | Chimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacc...

DRIVER (Spanish)

49

A seed-specific heat-shock transcription factor involved in developmental regulation during embryogenesis in sunflower.

Almoguera, Concepción; Rojas, A.; Díaz-Martín, J.; Prieto-Dapena, P.; Jordano, Juan
2002-11-15

Digital.CSIC (Spain)

50

A plant genetically modified that accumulates Pb is especially promising for phytoremediation

Gisbert, Carmina; Ros Palau, Roc; Haro Bailón, Antonio de; Walker, David J.; Bernal Calderón, M. Pilar; Serrano, Ramón; Navarro-Aviñó, Juan
2003-04-04

Digital.CSIC (Spain)

51

A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes

Sablowski, Robert W. M.; Moyano, Enriqueta; Culianez-Macía, Francisco A.; Schuch, Wolfgang; Cathie, Martin; Bevan, Michael
1994-01-01

Digital.CSIC (Spain)