Sample records for TECNICA DEL MACHO ESTERIL (sterile male technique)
from WorldWideScience.org

Sample records 1 - 3 shown.



2

Método de cultivo de larvas de moscas Lucilia sericata para terapia larval/ A CULTURE METHOD FOR Lucilia sericata FLY LARVAE FOR USE IN MAGGOT THERAPY

FIGUEROA, LUIS; FLORES, JORDANA; RODRÍGUEZ, SANDRA
2007-06-01

Resumen en español Los objetivos generales del estudio fueron: 1) Describir el ciclo de desarrollo de Lucilia sericata (Calliphoridae), en sus fases de huevo, larva, pupa e imago y 2) Adaptar una técnica para desinfectar nuevos de L. sericata y la posterior obtención de larvas estériles. El control del desarrollo se efectuó determinando diariamente sus distintos estadios. Para la técnica de desinfección, los huevos se sumergieron sucesivamente en soluciones de hipoclorito de sodio 0,5 (mas) % y formalina 10%, y luego lavados en NaCl 0,15 M. Para controlar la calidad de desinfección, una muestra de huevos fue distribuida en agar tripticasa de soya con 5% de sangre y en caldo común. El desarrollo de L. sericata en el laboratorio mostró que los huevos y su eclosión a larvas ocurrió (media ± desviación estándar) a las 15 ± 3,5 hr mientras que de larvas a pupas 138 ± 17 hr y de pupa a adultos 126 ± 21 hr. La técnica de desinfección de los huevos de L. sericata mostró un 87% de efectividad Resumen en inglés The general objectives of the present study were; 1) Describe the developmental cycle of Lucilia sericata (Calliphoridae) though the egg, larva, pupa, and imago stages, and 2) Develop a method for disinfecting the eggs of L. sericata. The developmental process of this fly was observed daily, determining times required for the appearance of each different stage. The disinfection technique included successive treatment of the eggs in solutions of 0.5% sodium hypochlorite an (mas) d 10% formalin after the eggs were obtained from experimentally crossing male and female flies in the laboratory. The eggs were then rinsed in sterile 0.15 MNaCl, and egg samples were spread-plated on trypticase soya agar containing 5% sheep blood, and also inoculated into in nutrient broth.The eggs of L. sericata hatched after 15 ± 3.5 hr, producing pupae after 138 ± 17 hr, and the adults emerged after 126 ± 21 hr. The disinfection technique used in the present study was 87% effective. It was concluded that that the complete development as well as the disinfection of the eggs could be carried out successfully in the laboratory for the production of larvae useful in maggot therapy

Scientific Electronic Library Online (Spanish)

3

Genetic, hormonal and environmental approaches for the control of reproduction in the European sea bass (Dicentrarchus labrax L.)

Zanuy, Silvia; Carrillo, Manuel; Felip, Alicia; Rodríguez, Lucinda; Blázquez, Mercedes; Ramos Jara, Jesús; Piferrer, Francesc
2001-11-01

Digital.CSIC (Spain)