Sample records for EMPALME (splicing)
from WorldWideScience.org

Sample records 1 - 20 shown. Select sample records:



1

El splicing alternativo del exón 5 de la citocromo p450 aromatasa podría ser un mecanismo de regulación de la producción de estrógenos en humanos/ Exon 5 alternative splicing of the cytochrome P450 aromatase could be a regulatory mechanism for estrogen production in humans

Pepe, Carolina M.; Saraco, Nora I.; Baquedano, María Sonia; Guercio, Gabriela; Vaiani, Elisa; Berensztein, Esperanza; Rivarola, Marco A.; Belgorosky, Alicia
2007-08-01

Resumen en español La enzima P450 aromatasa (P450Aro) participa en la síntesis de estrógenos a partir de andrógenos. La mutación c655G>A, descripta en forma heterocigota en una niña y en forma homocigota en un hombre adulto, ambos con déficit de aromatasa, genera la disrupción del sitio dador de splicing exón5-intrón5. Se ha postulado que la retención del intrón5 y la generación de una proteína truncada inactiva serían las consecuencias de esta mutación. Sorpresivamente, la p (mas) aciente presentó desarrollo espontáneo de mamas y niveles puberales de estradiol, sugiriendo una actividad aromatasa (AA) residual. En principio postulamos que la mutación c655G>A generaría la pérdida del exón5 con conservación del marco de lectura, generándose una proteína con menor actividad que podría explicar el déficit parcial. La expresión del ARNm sin exón5 (ARNm- E5) en linfocitos de la paciente sugiere una asociación entre la pérdida del exón y la presencia de la mutación; posteriormente confirmada realizando ensayos de splicing en células Y1. Sin embargo, la expresión del cDNAE5 en células Y1 presentó una AA nula que no explicaría un déficit parcial. La expresión del ARNm-E5 fue detectada en placenta, testículo y adrenal humanos como una variante de splicing normal. Estos resultados indicarían la ocurrencia de splicing alternativo (SA) en la zona codificante de P450Aro como un posible mecanismo regulador de la producción de estrógenos en tejidos esteroidogénicos humanos. La mutación c655G>A podría alterar los mecanismos fisiológicos reguladores del SA del exón5 favoreciendo su exclusión. De esta forma, bajos niveles de ARNm+E5 podrían expresarse aun en presencia de la mutación explicando el fenotipo de déficit parcial observado en la paciente. Resumen en inglés P450 aromatase (P450Aro), involved in androgen to estrogen conversion, is encoded by the CYP19 gene. P450Aro c655G>A mutation described in heterozygous form in a girl and in homozygous form in an adult male with P450Aro deficiency results in an aberrant splicing due to disruption of a donor splice site. A truncated inactive protein would be expected if intron5 is retained. Surprisingly, the girl described with this mutation showed spontaneous breast development and pubert (mas) al estradiol (E2) levels suggesting residual P450Aro activity (AA). Formerly, we postulate the in frame E5 skipping as a consequence of this mutation generating a protein with some degree of activity. When P450Aro mRNA expression was analysed from patient's lymphocytes, an aberrant spliced mRNA lacking E5 (-E5mRNA) was detected, suggesting an association between E5 skipping and the presence of the mutation. Splicing assays in Y1 cells confirmed this association. -Ex5 cDNA expression in Y1 cells resulted in an inactive protein that could not explain patient's phenotype. Exon 5 might be predicted as a poorly defined exon suggesting a susceptibility to splicing mutations and physiological alternative splicing (AS) events. Therefore, -Ex5mRNA was assessed as a natural occurring alternative transcript in normal human steroidogenic tissues. As P450Aro -E5mRNA expression was detected in human term placenta, prepubertal testis and prepubertal adrenal, we might speculate that AS of P450Aro coding region would occur in humans and would be involved in the complex AA regulation. Furthermore, tissue specific regulation of AS might suggest low expression of +E5mRNA from the c655G>A allele explaining residual AA evidenced in the affected girl.

Scientific Electronic Library Online (Spanish)

2

Fas Splicing Regulation during Early Apoptosis Is Linked to Caspase-mediated Cleavage of U2AF65

Izquierdo, José M.

Supplementary material available | U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor 65 kDa (U2AF65) is an essential splicing factor in the recognition of the pre-mRNA 3' splice sites during the assembly of the splicing commitment complex. We report here that U2AF65 is proteolyzed during a...

DRIVER (Spanish)

5

The functional modulation of epigenetic regulators by alternative splicing

Lois, Sergi; Blanco, Noemí; Martínez-Balbás, Marian; Cruz, Xavier de la

DRIVER (Spanish)

6

The functional modulation of epigenetic regulators by alternative splicing

Lois, Sergi; Blanco, Noemí; Martínez-Balbás, Marian; Cruz, Xavier de la
2007-07-25

Digital.CSIC (Spain)

8

Natural trans-splicing in carnitine octanoyltransferase pre-mRNAs in rat liver

Caudevilla, Concha; Serra, Dolors; Miliar, Angel; Codony, Carles; Asins, Guillermina; Bach, Montserrat; Hegardt, Fausto G.
1998-10-13

Digital.CSIC (Spain)

11

Proteomic analysis of phosphorylated nuclear proteins underscores novel roles for rapid actions of retinoic acid in the regulation of mRNA splicing and translation

Laserna Mendieta, Emilio José; Valero, M Luz; Sanz, Libia; Sánchez del Pino, Manuel M.; Calvete, Juan J.; Barettino, Domingo
2009-10-07

Digital.CSIC (Spain)

13

Genomic organization, chromosomal localization, alternative splicing,and isoforms of the human synaptosome-associated protein-23 gene implicated in vesicle-membrane fusion processes

Lazo, Pedro A.; Nadal, Marga; Ferrer, Milagros; Area, Estela; Hernández-Torres, Javier; Nabokina, S. M.; Mollinedo, Faustino; Estivill, Xavier
2001-03-06

Digital.CSIC (Spain)

15

A novel homozygous splice junction mutation in GPIIb associated with alternative splicing, nonsense-mediated decay of GPIIb-mRNA, and type II Glanzmann's thrombasthenia

González-Manchón, Consuelo; Arias-Salgado, Elena G.; Butta, Nora; Martín, G.; Rodríguez Martínez, Ramón B.

8 pages. | This work reports the study of a patient suffering a bleeding disorder clinically diagnosed as Glanzmann's thrombasthenia (GT). Immunoblotting and flow cytometric analysis showed a low (≤ 10% of control) platelet content of GPIIb–IIIa, confirming it was indeed a type II GT. The molecular ...

DRIVER (Spanish)

16

A novel homozygous splice junction mutation in GPIIb associated with alternative splicing, nonsense-mediated decay of GPIIb-mRNA, and type II Glanzmann's thrombasthenia

González-Manchón, Consuelo; Arias-Salgado, Elena G.; Butta, Nora; Martín, G.; Rodríguez Martínez, Ramón B.; Elalamy, I.; Parrilla, Roberto; Favier, R.
2003-04-25

Digital.CSIC (Spain)

18

Primate-specific spliced PMCHL RNAs are non-protein coding in human and macaque tissues

Schmieder, Sandra; Darré-Toulemonde, Fleur; Arguel, Marie-Jeanne; Delerue-Audegond, Audrey; Christen, Richard; Nahon, Jean-Louis
2008-12-09

Digital.CSIC (Spain)

19

Phytochelatin Synthases of the Model Legume Lotus japonicus. A Small Multigene Family with Differential Response to Cadmium and Alternatively Spliced Variants

Ramos Escribano, Javier; Clemente, María Rebeca; Naya, Loreto; Loscos, Jorge; Pérez Rontomé, Mari Carmen; Sato, Shusei; Tabata, Satoshi; Becana Ausejo, Manuel
2007-01-01

Digital.CSIC (Spain)

20

Characterization of alternatively spliced isoforms of AMPA receptor subunits encoding truncated receptors

Gomes, André R.; Ferreira, Joana S.; Paternain, Ana V.; Lerma Gómez, Juan; Duarte, Carlos B.; Carvalho, Ana Luísa
2008-02-01

Digital.CSIC (Spain)

21

SPACE: an algorithm to predict and quantify alternatively spliced isoforms using microarrays

Antón, Miguel A.; Gorostiaga, Dorleta; Guruceaga, Elizabeth; Segura, Víctor; Carmona-Sáez, Pedro; Pascual-Montano, Alberto; Pio, Rubén; Montuenga, Luis M.; Rubio, Angel
2008-02-29

Digital.CSIC (Spain)

23

Aromatase expression in the human temporal cortex

Garcí Yague, Josué; Muñoz, A.; Monasterio-Schrader, P. de; De Felipe-Oroquieta, Javier; García-Segura, Luis Miguel

13 pages, 8 figures, 2 tables.-- PMID: 16426763 [PubMed]. | The expression of the human cyp19 gene, encoding P450 aromatase, the key enzyme for estrogen biosynthesis, involves alternative splicing of multiple forms of exon I regulated by different promoters. Aromatase expression has been detected in...

DRIVER (Spanish)

24

Análisis mutacional del gen Homeobox de segmento muscular 1 (MSX1) en chilenos con fisuras orales/ Mutational analysis of the muscle segment homeobox gene 1 (MSX1) in Chilean patients with cleft lip/palate

Vieira, Alexandre R; Castillo Taucher, Silvia; Aravena, Teresa; Astete, Carmen; Sanz, Patricia; Tastets, María Eugenia; Monasterio, Luis; Murray, Jeffrey C
2004-07-01

Resumen en inglés Background: Mutations of the MSX1 gene may contribute to nonsyndromic forms of cleft lip and/or cleft palate. Aim: To search for mutations of MSX1 coding regions, including one highly conserved non-coding region in the single intron, among Chilean patients with cleft lip/palate. Patients and Methods: We studied 45 patients with cleft lip/palate and their parents. Oral mucosa samples were obtained with a swab. DNA was extracted and amplified by PCR. Results: Two missense m (mas) utations (G16D and G34A) were identified in this study that may be useful for future admixture studies. The G16D mutation appears to disrupt a possible splicing site and may contribute to clefting in this population. Conclusions: Rare MSX1 mutations are found in some cases of cleft lip and/or cleft palate but others remain to be found most likely in other regulatory regions of the gene (Rev Méd Chile 2004; 132: 816-22)

Scientific Electronic Library Online (Spanish)

25

RIBONUCLEASAS: [subtitle]SU POTENCIAL TERAPÉUTICO EN INFECCIONES VIRALES/ Ribonucleases: [subtitle]Theurapetical potential on Viral Infections

ÚSUGA, XIOMARA; RUGELES, MARÍA TERESA
2006-06-01

Resumen en español En la actualidad existe un gran interés por identificar proteínas o péptidos antimicrobianos que puedan ser herramientas terapéuticas que eviten el establecimiento o permitan el control de diferentes infecciones. Las ribonucleasas (RNasas), pertenecientes a la superfamilia Ribonucleasa A, son enzimas que participan en varios procesos fisiológicos, que van desde el procesamiento alternativo del RNA hasta la angiogénesis. Estas enzimas son expresadas por diferentes te (mas) jidos y exhiben especificidades variables contra diferentes sustratos de RNA. El potencial terapéutico de las RNasas se ha sugerido en procesos oncogénicos; adicionalmente, se ha descrito que tienen actividad antiviral directa y el potencial de activar células del sistema inmune innato induciendo su maduración y la producción de citoquinas proinflamatorias. Nuestro grupo de investigación ha realizado estudios que señalan la capacidad de cuatro RNasas recombinantes: EDN, 4EDN, RNasa A y angiogenina de inhibir la replicación del virus de la inmunodeficiencia humana tipo 1 en linfocitos T de sangre periférica activados. En este artículo se revisará la clasificación de las ribonucleasas que constituyen la superfamilia RNasa A y se describirá, en forma detallada, lo que se conoce de la función biológica, acción antiviral y mecanismo de acción de las RNasas a las que se les ha reportado actividad antiviral. Resumen en inglés Currently, there is a great interest to identify proteins or antimicrobial peptides to be included in the therapeutic arsenal for preventing different infectious diseases. Ribonucleases (RNases) that belong to the Ribonuclease A superfamily participate in several physiologic processes, from alternative splicing of RNA to organogenesis. These enzymes are expressed by various tissues and exhibit variable specificities against different RNA substrates. The therapeutic potent (mas) ial of RNases has been suggested for oncogenic processes; in addition, direct antiviral activity and the potential to activate cells from the innate immune system, inducing their maturation and release of proinflammatory cytokines have been also associated with these enzymes. Our research team, have carried out studies that indicate the ability of four recombinant RNases: EDN, 4EDN, RNase A and angiogenin to inhibit HIV1 replication in activated peripheral blood T lymphocytes. In this article we review the classification of RNases that belong to the Ribonucleases A superfamily; we describe in detail what is known regarding the biologic function, inhibitory activity and mechanism of action of the RNases recognized by their antiviral activity.

Scientific Electronic Library Online (Spanish)

26

Manipulación genética y el estudio del parásito protozoario Leishmania/ Genetic manipulation and the study of the protozoan parasite Leishmania

Cortázar, Tania M; Walker, John
2004-12-01

Resumen en español Durante los últimos 15 años se ha dado paso al entendimiento de muchos aspectos de la genómica funcional de Leishmania gracias a los avances en la metodología de transfección de ADN dentro de la célula de este protozoario, la eliminación y la complementación de genes por medio de recombinación homóloga y las estrategias para la selección de células transfectadas. Estos acercamientos tienen el potencial de brindar información sobre la expresión génica y la f (mas) unción de las proteínas en el contexto del parásito intacto. Dado que el genoma de Leishmania muestra una carencia acentuada de los factores conocidos de iniciación de la transcripción y que la expresión génica está regulada casi completamente a nivel postranscripcional (a través del empalme de los ARNm y los mecanismos que involucran el procesamiento diferencial de la región no traducida 3' del ARNm (3’UTR), la transfección génica representa una herramienta útil para la identificación y el análisis funcional de los genes de interés así como de los mecanismos que dirigen su regulación. El desarrollo de los sistemas de manipulación genética también ha abierto nuevos horizontes para la identificación de genes esenciales involucrados en la virulencia, la supervivencia intracelular y la resistencia a drogas de Leishmania, así como para la validación de proteínas específicas del parásito como nuevos blancos quimio e inmunoterapéuticos. En esta revisión presentamos los avances más recientes en el campo de la manipulación genética en Leishmania, los cuales permiten análisis estructurales, funcionales y de fenotipo, por medio de la eliminación y complementación génica a través de la transfección transitoria o permanente de genes en este parásito. Resumen en inglés During the last 15 years, many aspects of the functional genomics of Leishmania have been revealed due to advances in DNA transfection, gene disruption and complementation through homologous recombination, and efficient strategies for the selection of transfected cells. These strategies have provided information about gene expression and protein function in the context of the intact parasite. The genome of Leishmania shows a marked deficiency of known transcription initia (mas) tion factors, and gene expression is regulated almost entirely at the posttranscriptional level through trans-splicing of mRNAs and novel control mechanisms involving differential processing of 3' - untranslated regions (3'-UTRs) of mRNAs. Therefore, gene transfection represents a useful tool for the identification and functional analysis of genes of interest as well as the mechanisms that direct their regulation. The development of genetic manipulation systems has provided opportunities for the study of genes involved in virulence, intracellular survival and drug resistance of Leishmania, as well as for the functional validation of specific parasite proteins as new chemo- and immunotherapeutic targets. The current review presents recent advances in genetic manipulations that permit structural, functional and phenotypic analyses and by means of gene deletion and complementation using the methods of gene transfection.

Scientific Electronic Library Online (Spanish)

27

Detección de mutaciones de los genes hMLH1 y hMSH2 del sistema de reparación de malos apareamientos del ADN en familias colombianas sospechosas cáncer colorrectal no polipósico hereditario (síndrome de Lynch)/ Detection mutations in the DNA mismatch repair genes of hMLH1 and hMSH2 genes in Colombian families with suspicion of hereditary non-polyposis colorectal carcinoma (Lynch syndrome)

Gómez, Andrea; Salguero; García, Herbert; Aristizábal, Fabio; Gutiérrez, Óscar; Ángel, Luis Alberto; Padrón, Jorge; Martínez, Carlos; Martínez, Humberto; Malaver, Omar; Barvo, Rosa; Giraldo, Alejandro
2005-09-01

Resumen en español Introducción. El cáncer colorrectal es la segunda causa de morbilidad y mortalidad por cáncer en los países desarrollados. En Colombia es la quinta causa de muerte entre los diferentes cánceres. Cerca del 75% de éstos corresponde a cánceres esporádicos, alrededor del 25% son familiares, y son claramente hereditarios el 5%. De éstos, el más importantes es el cáncer colorrectal no polipósico hereditario o síndrome de Lynch. Objetivo. Analizar los dos genes más (mas) importantes involucrados en el síndrome de Lynch, el hMLH1 y el hMSH2. Materiales y métodos. En 17 familias colombianas que cumplían con los criterios de Ámsterdam II o las pautas de Bethesda, se analizaron por SSCP los 35 exones de estos dos genes y las variantes electroforéticas se secuenciaron. Resultados. Se detectaron 8 mutaciones de línea germinal en las familias analizadas, 7 en el gen hMLH1 y 1 en hMSH2, y se encontró una tasa de detección de mutaciones del 47%. Seis de las 8 mutaciones encontradas en este estudio han sido previamente reportadas en la literatura. Un cambio de una base en el sitio donador de empalme en el exón 9 del gen hMLH1 (G>A) (dos familias), un cambio A>G en el codón 755 del exón 17, y un cambio G>A en el exón 18. Se detectaron dos nuevas mutaciones, una en el exón 17, un cambio C>T en el codón 640, y una deleción de TG en el codón 184 del exón 3 del gen hMSH2. También se detectó en dos familias un polimorfismo del intrón 13 del hMLH1. Conclusión. Este es el primer estudio realizado en Colombia que detecta mutaciones en el síndrome de Lynch y pretende establecer un programa integral de manejo y prevención. Resumen en inglés Introduction. Colorectal cancer (CRC) is the second highest cause of cancer mortality in developed countries. In Colombia, CRC ranks fifth as a cause of cancer death. Approximately 75% of CRC appear to be spontaneous and 25% are familial, with 5% of the latter clearly hereditary. Of these, hereditary non-polyposis colorectal carcinoma (HNPCC)-or Lynch syndrome is the most important. Objective. Herein, the two most important genes involved in Lynch syndrome, the hMLH1 and (mas) hMSH2 were analyzed for presence of mutations. Materials and methods. Seventeen Colombian families that fulfilled the Amsterdam II criteria or Bethesda guidelines for Lynch syndrome were selected. The of 35 exons of hMLH1 and hMSH2 genes were screened by SSCP and those with electrophoretic variants were sequenced. Results. Eight germinal mutations were detected, corresponding to a 47% detection mutation rate. Six of the eight mutations have previously been reported. These consisted of the following mutations: a single base substitution at the donor splicing site of exon 9, a single base substitution (A>G) at codon 755 of the exon 17, and another single base substitution (G>A) at codon 681 of exon 18. The two novel mutations consisted of a single base substitution (C>T) at codon 640 of exon 17 of the hMLH1 gene and a two-nucleotide deletion (TG) at codon 184 of exon 3 of hMSH2 gene. In addition, two families were observed with a polymorphism in the intron 13 (G>A) nt 1558+14, of hMLH1 gene. Conclusions. This study represented the first survey for detecting mutations associated with Lynch syndrome in Colombia, and is intended to lead to the establishment of a management and prevention program.

Scientific Electronic Library Online (Spanish)

28

Análisis molecular del gen GABRB3 en pacientes con autismo: Estudio exploratorio/ Molecular analysis of the GABRB3 gene in Autistic patients: An exploratory study

Solís-Añez, Ernesto; Delgado-Luengo, Wilmer; Borjas-Fuentes, Lisbeth; Zabala, William; Arráiz, Nailet; Pineda, Lennie; Portillo, María Gabriela; González-Ferrer, Sandra; Chacín, José Antonio; Peña, Joaquín; Montiel, Cecilia; Morales, Alisandra; Rojas de Atencio, Alicia; Cañizales, Jenny; González, Richard; Miranda, Luis Eduardo; Abreu, Nivia; Delgado, Juana
2007-06-01

Resumen en español El autismo es un trastorno del desarrollo caracterizado por deterioro de la interacción social, la comunicación, y comportamiento estereotipado. Los estudios de familias y gemelos han demostrado predisposición genética al autismo. Existe evidencia (ligamiento y asociación genética, bioquímica, anatomopatológica, funcional y citogenética) de que el gen de la subunidad β3 del receptor de GABA-A (GABRB3), en 15q11-q13, es un candidato de susceptibilidad al auti (mas) smo. Con el objetivo de identificar nuevas variantes en este gen, se estudiaron 18 pacientes con autismo idiopático, utilizando un tamizaje de gen candidato. Se realizó el análisis molecular (SSCP/secuenciación) de los 10 exones con sus correspondientes regiones intrónicas flanqueantes. No se identificaron mutaciones no sinónimas en las regiones codificantes, pero se identificaron 4 polimorfismos de nucleótido simple (SNP). El primer SNP representó una mutación silente p.P25P en el exon 1a, encontrada en 33,33% de los pacientes. El Segundo SNP: IVS3+13C > T (a 5 b de la secuencia consenso 5’ del intrón) fue encontrado en 44,44% de los pacientes, mientras que fué identificado en 16.67% de los controles. El 33,33% de los pacientes presentaron simultáneamente ambas variantes, y aunque el 16,67% de los controles también poseían la misma combinación, el 66,66% de los pacientes con esos alelos tenían antecedentes familiares de autismo. El tercer y cuarto SNP: IVS5+40T > G e IVS7-70A > G fueron identificados en dos pacientes diferentes. Ninguno de los 3 últimos SNPs ha sido reportado en la base de datos de SNP (dbSNP). La cercanía del SNP: IVS3+13C > T con la secuencia consenso y de interacción con la nucleorribonucleoproteína U1, pudiera alterar la maduración normal del pre-ARNm, en concordancia con la evidencia de niveles bajos del receptor GABA-A en cerebros de pacientes con autismo, pudiendo entonces tratarse, de una variante común, que por sí sola no causaría un efecto fenotípico, pero que en conjunto con otras variantes en el mismo gen, en genes relacionados o, con cambios epigenéticos, pudieran explicar el fenotipo autista y su gran heterogeneidad. Resumen en inglés Autism is a complex neurodevelopmental disorder characterized by impairment of social interaction, language, communication, and stereotyped, repetitive behavior. Genetic predisposition to Autism has been demonstrated in families and twin studies. There is evidence (linkage and genetic association, biochemical, neuropathological, functional and cytogenetic) that the gamma-amino-butyric acid receptor beta 3 subunit gene (GABRB3) at 15q11-q13 is a susceptibility candidate ge (mas) ne for Autism. The aim of this exploratory study was to identify new variants of this gene. We performed the molecular analysis (SSCP/Sequencing) of 10 exons and its intronic flanking regions of GABRB3, using a candidate gene screening approach in 18 idiopathic autistic patients. We did not find non-synonymous mutations at the encoding regions, but we identified four SNP (Single Nucleotide Polymorphism). The first one, represented a silent mutation p.P25P in exon 1a and was found in 33.33% of the patients. The second one: IVS3+13C > T (5b far from the intron 5’ consensus sequence), was found in 44.44% of the patients, while it was also identified in 16.67% of the controls. Simultaneously, 33.33% of the patients had both variants, and although, 16.67% of the controls also had the same combination of variants, 66.66% of the patients with those alleles had a familiar history of Autism. The third and fourth SNP: IVS5+40T > G and IVS-70A > G were identified in two different patients. None of the last three SNPs have been reported at the SNP database (dbSNP). The proximity of SNP: IVS3+13C > T with the consensus and interaction sequence with U1 nucleoriboprotein, could disturb the normal splicing of mRNA. This is in agreement with the evidence of lower levels of GABA-A receptors in autistic brains; so, it could be a common variant, that by itself could not cause a phenotypic effect, but joined to other variants with the same gene, in different related genes or with epigenetic changes, could explain the autistic phenotype and its heterogeneity.

Scientific Electronic Library Online (Spanish)

30

The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets

Díaz-Moreno, Irene; García-Mayoral, María Flor; Hollingworth, David; Ramos, Andres
2008-08-06

Digital.CSIC (Spain)

31

The gene transformer-2 of Anastrepha fruit flies (Diptera, Tephritidae) and its evolution in insects

Sarno, Francesca; Ruiz Lorenzo, María Fernanda; Eirín-López, José María; Perondini, André L. P.; Selivon, Denise; Sánchez Rodríguez, Lucas
2010-05-13

Digital.CSIC (Spain)

32

The gene transformer of anastrepha fruit flies (Diptera, tephritidae) and its evolution in insects

Ruiz Lorenzo, María Fernanda; Milano, Andreina; Salvemini, Marco; Eirín-López, José María; Perondini, André L. P.; Selivon, Denise; Polito, Catello; Saccone, Giuseppe; Sánchez Rodríguez, Lucas
2007-11-28

Digital.CSIC (Spain)

33

The Structure of the C-Terminal KH Domains of KSRP Reveals a Noncanonical Motif Important for mRNA Degradation

García-Mayoral, María Flor; Hollingworth, David; Masino, Laura; Díaz-Moreno, Irene; Kelly, Geoff; Chou, Chu-Fang; Chen, Ching-Yi; Ramos, Andres
2007-04-01

Digital.CSIC (Spain)

35

The Long and Short Flavodoxins. I. i. the role of the differentiating loop in apoflavodoxin structure and fmn

López-Llano, Jon; Maldonado, Susana; Bueno, Marta; Lostao, Anabel; Jiménez, María Ángeles; Lillo, M. Pilar; Sancho, Javier
2004-08-17

Digital.CSIC (Spain)

36
37

SADB phosphorylation of γ-tubulin regulates centrosome duplication

Alvarado-Kristensson, María; Rodríguez, María Josefa; Silió, Virginia; Valpuesta, José M.; Carrera, Ana C.
2009-08-02

Digital.CSIC (Spain)

38

Regulation of expression of two LY-6 family genes by intron retention and transcription induced chimerism

Calvanese, Vincenzo; Mallya, Meera; Campbell, R. Duncan; Aguado, Begoña
2008-09-25

Digital.CSIC (Spain)

39

Regulation of cAMP phosphodiesterase mRNAs expression in rat brain by acute and chronic fluoxetine treatment. An in situ hybridization study

Miró, Xavier; Pérez-Torres, Silvia; Artigas, Francesc; Puigdomènech, Pere; Palacios, José M.; Mengod Los Arcos, Guadalupe
2002-12-01

Digital.CSIC (Spain)

41

Rac1b and reactive oxygen species mediate MMP-3-induced EMT and genomic instability

Radisky, Derek C.; Levy, Dinah D.; Littlepage, Laurie E.; Liu, Hong; Nelson, Celeste M.; Fata, Jimmie E.; Leake, Devin; Godden, Elizabeth L.; Albertson, Donna G.; Nieto, M. Ángela; Werb, Zena; Bissell, Mina J.
2005-07-07

Digital.CSIC (Spain)

42
43

Polimerasa lambda de ADN y sus usos

Blanco, Luis; Bernad, Antonio; García Cabezas, Miguel Ángel; Domínguez, Orlando
2006-09-01

Digital.CSIC (Spain)

44

Phosphorylation-mediated unfolding of a KH domain regulates KSRP localization via 14-3-3 binding

Díaz-Moreno, Irene; Hollingworth, David; Frenkiel, Thomas A.; Kelly, Geoff; Martin, Stephen; Howell, Steven; García-Mayoral, María Flor; Gherzi, Roberto; Briata, Paola; Ramos, Andres
2009-02-08

Digital.CSIC (Spain)

45

Pendred syndrome in two Galician families: insights into clinical phenotypes through cellular, genetic, and molecular studies

Palos, Fernando; García-Rendueles, María E. R.; Araujo-Vilar, David; Obregón, María Jesús; Calvo, Rosa M.; Cameselle, José C.; Bravo, Susana B.; Pérez-Guerra, Óscar; Loidi, Lourdes; Czarnocka, Barbara; Álvarez, Paula; Refetoff, Samuel; Domínguez-Gerpe, Lourdes; Álvarez, Clara V.; Lado-Abeal, Joaquín
2008-01-01

Digital.CSIC (Spain)

46

P19 H-Ras Induces G1/S Phase Delay Maintaining Cells in a Reversible Quiescence State

Camats, Maria; Mariette, Kokolo; Heesom, Kate J.; Ladomery, Michael; Bach-Elias, Montse
2009-12-30

Digital.CSIC (Spain)

47
48

Nuclear bodies domain changes with microspore reprogramming to embryogenesis

Seguí-Simarro, José M.; Bárány, Ivett; Suárez, R.; Fadón, Begoña; Sánchez-Testillano, Pilar; Risueño, María del Carmen
2006-01-01

Digital.CSIC (Spain)

51

LPS-induced down-regulation of NO-sensitive guanylyl cyclase in astrocytes occurs by proteasomal degradation in nuclear bodies

Berciano, María T.; Baltrons, María Antonia; Pifarré, Paula; Lafarga, Miguel; García, Agustina
2007-07-25

Digital.CSIC (Spain)

53

Integration of RNA processing and expression level control modulates the function of the Drosophila Hox gene Ultrabithorax during adult development.

de Navas, Luis F.; Reed, Hilary; Akam, Michael; Barrio, Rosa; Alonso, Claudio R.; Sánchez-Herrero, Ernesto
2011-01-01

Digital.CSIC (Spain)

55
56

Evolution of the insulin receptor family and receptor isoform expression in vertebrates

Hernández Sánchez, Catalina; Mansilla, Alicia; Pablo Dávila, Flora de; Zardoya, Rafael
2008-02-29

Digital.CSIC (Spain)

57

Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector

Fuerstenberg, S.; Beug, H.; Introna, M.; Khazaie, K.; Muñoz, A.; Ness, S.; Nordström, Kristina; Sap, J.; Stanley, I.; Zenke, M.; Vennström, Björn
1990-12-01

Digital.CSIC (Spain)

58

Differentiating plant cells switched to proliferation remodel the functional organization of nuclear domains

Sánchez-Testillano, Pilar; González-Melendi, Pablo; Coronado, María José; Seguí-Simarro, José M.; Moreno, Miguel A.; Risueño, María del Carmen
2005-01-01

Digital.CSIC (Spain)

59

Differential Role of Human Choline Kinase α and β Enzymes in Lipid Metabolism: Implications in Cancer Onset and Treatment

Gallego Ortega, David; Ramírez de Molina, Ana; Ramos, Maria Angeles; Valdés Mora, Fátima; Barderas, Maria Gonzalez; Sarmentero Estrada, Jacinto; Lacal Sanjuán, Juan Carlos
2009-11-12

Digital.CSIC (Spain)

60

Different sox17 transcripts during sex differentiation in sea bass, Dicentrarchus labrax

Navarro-Martín, Laia; Galay-Burgos, Malyka; Sweeney, Glen E.; Piferrer, Francesc
2009-02-27

Digital.CSIC (Spain)

61

Dido gene expression alterations are implicated in the induction of hematological myeloid neoplasms

Fütterer, Agnes; Campanero, Miguel R.; Leonardo, Esther; Criado, Luis M.; Flores, Juana María; Hernández, Jesús M.; San Miguel, Jesús F.; Martínez-Alonso, Carlos
2005-09-01

Digital.CSIC (Spain)

63

DNA Polymerase lambda and uses thereof.

Blanco Davila, Luis; Bernal, A.; Domínguez, Orlando; García Díaz, Miguel
2006-09-01

Digital.CSIC (Spain)

64

Computational approaches to study transcriptional regulation in the human genome

Luscombe, Nicholas M.; Gómez Puertas, Paulino; Vaquerizas Erdocia, Juan Manuel
2008-01-01

Digital.CSIC (Spain)

65

Characterization of the human DYRK1A promoter and its regulation by the transcription factor E2F1

Maenz, Barbara; Hekerman, Paul; Vela, Eva M.; Galcerán, Juan; Becker, Walter
2008-03-26

Digital.CSIC (Spain)

66

Biogenesis of mRNPs: integrating different processes in the eukaryotic nucleus

Luna, Rosa; Gaillard, Hélène; González-Aguilera, Cristina; Aguilera, Andrés
2008-04-22

Digital.CSIC (Spain)

67

Aromatase expression in the human temporal cortex

Garcí Yague, Josué; Muñoz, A.; Monasterio-Schrader, P. de; De Felipe-Oroquieta, Javier; García Segura, Luis Miguel; Azcoitia, I.
2006-01-19

Digital.CSIC (Spain)

68

An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R

Lospitao, Eva Pilar; Pérez-Ferreiro, Carmen; Gosálbez, Altea; Alonso, Miguel A.; Correas, Isabel
2008-12-04

Digital.CSIC (Spain)

70

A rare missense mutation in a type 2 diabetes patient decreases the transcriptional activity of human sterol regulatory element binding protein-1

Vernia, Santiago; Eberlé, Delphine; Hernández Mijares, Antonio; Foufelle, Fabienne; Casado, Marta
2006-02-20

Digital.CSIC (Spain)

71

A novel point variant in NTRK3, R645C, suggests a role of this gene in the pathogenesis of Hirschsprung disease

Fernández, Raquel M.; Sánchez-Mejías, Avencia; Mena, Marcela; Ruiz-Ferrer, Macarena; López-Alonso, Manuel; Antiñolo, Guillermo; Borrego, Salud
2009-01-01

Digital.CSIC (Spain)