Sample records for PSE(PARTICULAS SOLARES ENERGETICAS) (spe)
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1

Routine and sensitive SPE-HPLC method for quantitative determination of pheophytin a and pyropheophytin a in olive oils

Hornero Méndez, Dámaso; Gandul Rojas, Beatriz; Mínguez Mosquera, María Isabel

6 pages, 1 figure, 2 tables.-- Printed version published Oct 2005.-- Issue title: Third International Congress on Pigments in Food (Quimper, France, Jun 14-17, 2005). | A sensitive and specific HPLC method with tandem diode array-fluorescence detection (DAD-FL) has been developed and validated for t...

DRIVER (Spanish)

2

Routine and sensitive SPE-HPLC method for quantitative determination of pheophytin a and pyropheophytin a in olive oils

Hornero Méndez, Dámaso; Gandul Rojas, Beatriz; Mínguez Mosquera, María Isabel
2005-07-01

Digital.CSIC (Spain)

4

Highly selective sample preparation and gas chromatographic–mass spectrometric analysis of chlorpyrifos, diazinon and their major metabolites in sludge and sludge-fertilized agricultural soils

Díaz-Cruz, M. Silvia; Barceló, Damià

6 pages, 3 figures, 3 tables.-- PMID: 16919644 [PubMed].-- Printed version published Nov 3, 2006. | A pressurized liquid extraction (PLE) solid-phase extraction (SPE) gas chromatography–mass spectrometry (GC–MS) method was developed for the determination of two model organophosphorus pesticides (OPP...

DRIVER (Spanish)

5

Advantages and limitations of on-line solid phase extraction coupled to liquid chromatography–mass spectrometry technologies versus biosensors for monitoring of emerging contaminants in water

Rodríguez-Mozaz, Sara; López de Alda, María J.; Barceló, Damià

19 pages, 5 tables, 1 figure.-- PMID: 17275010 [PubMed].-- Printed version published on Jun 8, 2007. | On-line solid phase extraction (SPE) coupled to liquid chromatography–mass spectrometry (LC–MS) and biosensors are advanced technologies that have found increasing application in the analysis of en...

DRIVER (Spanish)

6

Fully Automated Determination in the Low Nanogram per Liter Level of Different Classes of Drugs of Abuse in Sewage Water by On-Line Solid-Phase Extraction-Liquid Chromatography-Electrospray-Tandem Mass Spectrometry

Postigo, Cristina; López de Alda, María J.; Barceló, Damià

12 pages, 6 figures.-- Printed version published on May 1, 2008. | The present work describes the first fully automated method, based on on-line solid-phase extraction (SPE)-liquid chromatography-electrospray-tandem mass spectrometry, developed for the determination of drugs of abuse (17 compounds a...

DRIVER (Spanish)

7

Analysis of pesticides and metabolites in Spanish surface waters by isotope dilution gas chromatography/mass spectrometry with previous automated solid-phase extraction: Estimation of the uncertainty of the analytical results

Planas Pastor, Carles; Puig, Alejandra; Rivera Aranda, Josep; Caixach Gamisans, Josep

11 pages, 2 figures.-- PMID: 16962600 [PubMed].-- Printed version published Oct 27, 2006. | A method based on isotope dilution gas chromatography/mass spectrometry (GC/MS) with automated solid-phase extraction (SPE) is described for the analysis of 32 pesticides and metabolites in surface waters. Th...

DRIVER (Spanish)

8

Parámetros hemostáticos en placenta de pacientes con embarazo normal y con preeclampsia severa/ Hemostatic system parameters of placental extracts in normal pregnancy and severe preeclampsia

López-Ramírez, Ysabel; Carvajal, Zoila; Arocha-Piñango, Carmen Luisa
2006-09-01

Resumen en español Con el objeto de buscar el mejor entendimiento del mecanismo hemostático en la preeclampsia, se estudiaron extractos de placentas obtenidos de 26 mujeres embarazadas normales (EN) y 12 pacientes con preeclampsia severa (PES), en los cuales se determinaron trombomodulina (TM), factor tisular (FT), activador tisular del plasminógeno (t-PA), inhibidores del activador del plasminógeno 1 y 2 (PAI-1 y PAI-2) y el inhibidor del factor tisular (TFPI). Los resultados mostraron (mas) concentraciones similares de FT, TM y PAI-2 en ambos grupos, t-PA incrementado no significativamente y el TFPI y el PAI-1 presentaron un incremento significativo en las placentas de PES. Resumen en inglés To better understand the role of the hemostatic mechanism in preeclampsia, placental extracts obtained from 26 normal pregnant women (NP) and 12 patients with severe pre-eclampsia (SPE) were analyzed to determine thrombomodulin (TM), tissue factor (TF), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor (PAI) 1 and 2, and TF pathway inhibitor (TFPI). The results showed similar concentrations of TF, TM and PAI-2 in both groups, while tPA increased no significantly and TFPI and PAI-1 increased significantly in SPE placentas.

Scientific Electronic Library Online (Spanish)

9

Teoría de la escalera térmica/ Thermic stairways theory

Macchiavello C, Marco Aurelio
2009-08-01

Resumen en español En el presente artículo se plantea una posible relación entre la temperatura corporal de diferentes especies animales y la presentación de la Gripe aviar. Se postula que los diferentes valores de temperatura corporal podrían actuar como un mecanismo favorecedor de las infecciones por virus Influenza, mediante un mecanismo de tipo ad-aptativo de los viriones en las diversas especies animales susceptibles a la infección, incluyendo al humano Resumen en inglés In this article we raise the possibility of a potential relationship between body temperature of different spe-cies of animáis and the Avian Flu. We hypothesize that the different valúes of body temperature could act as an enabling mechanism for Influenza virus infection through an adaptative mechanism of the virions in the species susceptible to infection, including human beings

Scientific Electronic Library Online (Spanish)

10

Un método simple para la recolección, extracción y medición de catecolaminas en orina de adultos mayores/ A simple method to collect, extract and measure urinary catecholamines in elderly people

Benavides Varela, Silvia; Fornaguera Trías, Jaime
2007-12-01

Resumen en español La medición de catecolaminas en orina ha sido utilizada tanto para diagnóstico como para la investigación. Este trabajo propone algunas modificaciones y condiciones estandarizadas para llevar a cabo la recolección de muestras de orina y la extracción y cuantificación de las catecolaminas utilizando cromatografía líquida de alto desempeño con detección electroquímica (HPLC-EC). Se probaron distintos métodos de preservación de la muestra durante la recolección (mas) , y se encontró que no es necesario utilizar ningún preservante o refrigerante para mantenerla estable. Se utilizó también la extracción en fase sólida (EFS) en la que, al modificar variables como la velocidad de elusión y la cantidad del eluyente final, se obtuvieron porcentajes de recuperación de 85,8% para dopamina, 88% para epinefrina y 96,9% para norepinefrina. En la medición por cromatografía, los coeficientes de variación fueron menores al 10% en la evaluación entre días y entre corridas; el error fue 2,5% en todos los casos. Se probó, además, que el método es lineal (r²Z0,9882; a Resumen en inglés Catecholamines in plasma and urine have been widely used for clinical diagnosis and research. The present paper proposes simple changes to collect, extract and quantify catecholamines among elderly people, and to obtain more accurate results using HPLC-EC. Refrigeration and acid were tested as preserving methods during urine collection. A pre-treatment with SPE was used to clean samples, and some variables were changed in order to improve recovery previous to chromatograp (mas) hy analysis. No significant differences were found in sample catecholamines concentration using different preservation methods, or even in samples without refrigeration or acid during collection. Recovery obtained after changing some variables in SPE was 88.0% in epinephrine, 85.8% in dopamine and 96.9% in norepinephrine. VC was less than 10% between-days and between-run evaluation in all catecholamines, and error was 2.5%. Calibration curves were linear (r²Z0.9882; a

Scientific Electronic Library Online (Spanish)

11

Metabolitos del efavirenz como probable causa de falsos-positivos en test inmunológico para benzodiacepinas en orina/ False-positive immunoassay results for urine benzodiacepines probably caused by efavirenz metabolites

Quiroga, Patricia N.; Mirson, Daniel J.E.; Ridolfi, Adriana S.; Fuentes, Silvia; De Cristófano, María de los Angeles; Navoni, Julio; Villaamil Lepori, Edda C.
2007-12-01

Resumen en español En el tratamiento del síndrome de inmunodeficiencia adquirida (SIDA) una de las drogas antirretrovirales usadas es el efavirenz (EFV). Existe una asociación entre el consumo de drogas de abuso y la probabilidad de adquirir el SIDA, razón por la cual se solicita su investigación en orina. Como método de screening para detectar el consumo de estas drogas se utilizan habitualmente los ensayos inmunológicos. Una característica especial de estos métodos son las reaccio (mas) nes cruzadas que pueden presentarse con sustancias estructuralmente relacionadas dando origen a resultados falsos positivos. Al analizar 18 muestras de orina de pacientes con SIDA, se observó un 78% de resultados falsos positivos para benzodiacepinas (BZD) cuando fueron analizados mediante el ensayo inmunológico Triage® (Ascend Multi Immune Assay). El estudio confirmatorio por cromatografía gaseosa acoplada a espectrometría de masa (GC-MS) reveló la ausencia de BZD en todos los casos y el 100% de los resultados falsos positivos observados correspondieron a las muestras de los pacientes tratados con EFV. Con el propósito de dilucidar el origen de esta reacción cruzada fueron aislados el EFV y sus metabolitos de las muestras de orina, mediante extracción en fase sólida (SPE) y cromatografía en capa delgada de alta resolución (HPTLC), e identificados por cromatografía gaseosa- espectrometría de masa (GC-MS y GC-MS/MS/MS). Los resultados obtenidos en este estudio indicarían que los metabolitos del EFV (8-OH-EFV y/o 7-OH-EFV) y no el EFV podrían ser los responsables de la reacción cruzada observada en el ensayo inmunológico. Resumen en inglés Efavirenz (EFV) is an antiretroviral drug used in the acquired immunodeficiency syndrome (AIDS) treatment. Immunoassay techniques have been widely used for abuse drug screening test. The presence of structurally related substances in urine samples can interfere by cross reactions causing false positive results. High percentage of false positive results (78%) for benzodiazepines (BDZ) had been established for 18 urine samples assayed by immunoassay test Triage® (Ascend Mu (mas) lti Immune Assay). Gas chromatography-mass spectrometry (GC-MS) evaluation was negative for BDZ for all cases. One hundred percent (100%) of the positive results came from patients treated with EFV. With the aim to determine the cause of this cross- reaction, EFV and its metabolites were isolated by solid phase extraction (SPE) and high performance thin-layer chromatography (HPTLC) and then, identified by gas chromatography mass spectrometry (GC-MS and GC-MS/MS/MS). GC-MS/MS/MS analysis showed that EFV metabolites (8-OH-EFV and/or 7-OH-EFV) could probably be responsible for the cross reaction observed in the immunologic assays.

Scientific Electronic Library Online (Spanish)

12

Caracterización molecular de Streptococcus pyogenes causantes de enfermedad invasora y síndrome de shock tóxico estreptocócico/ Molecular characterization of Streptococcus pyogenes from invasive disease and streptococcal toxic shock syndrome episodes

Traverso, F.; Sparo, M.; Rubio, V.; Sáez Nieto, J.A.
2010-02-01

Resumen en español Streptococcus pyogenes es el agente causal de varias enfermedades comunes entre las que se incluyen la faringoamigdalitis, la escarlatina y el impétigo. Sin embargo, en las últimas décadas se ha registrado mundialmente un resurgimiento de casos de enfermedad invasora y síndrome de shock tóxico estreptocócico (SSTE). El propósito del presente trabajo fue estudiar la diversidad genética, los factores de virulencia (genes spe, sme, ssa) y la sensibilidad a los antibi (mas) óticos de 10 cepas de S. pyogenes causantes de enfermedad invasora y SSTE. Los aislamientos fueron recuperados de hemocultivos de pacientes internados en el Hospital Santamarina y en la Nueva Clínica Chacabuco (Tandil, Argentina) entre diciembre de 2000 y abril de 2005. Predominaron 2 patrones de electroforesis en campo pulsante. El más frecuente comprendió 5 aislamientos del tipo emm1-T1, con perfil de toxinas speA, speB, speF, speG y smeZ. El segundo patrón más frecuente incluyó 2 aislamientos tipo emm3-TNT (speB, speF, speG). Estos dos tipos (emm1 y emm3) fueron los prevalentes en las infecciones invasoras. Las otras tres cepas correspondieron a los tipos emm49-TNT (speB, speC, speF, speG), emm75-T25 (speB, speF, speG) y emm83-TNT (speB, speF, speG, ssa, smeZ). Se encontró diversidad genética entre las cepas aisladas, pero todos los aislamientos fueron sensibles a penicilina, cefotaxima, eritromicina, clindamicina, cloranfenicol, tetraciclina y rifampicina. Por tal motivo, aún es válido el tratamiento empírico con penicilina asociada a clindamicina. Resumen en inglés Streptococcus pyogenes causes a variety of common human diseases, including pharyngitis, scarlet fever and impetigo. Nevertheless, the past decades have witnessed a worldwide resurgence in invasive disease and streptococcal toxic shock syndrome (STSS). The objective of the present study is to evaluate the genetic diversity, virulence gene distribution (spe, sme and ssa genes) and susceptibility pattern of 10 S. pyogenes isolates causing invasive disease and STSS. The isol (mas) ates were recovered from blood cultures of hospitalized patients at Hospital Santamarina and Nueva Clínica Chacabuco, Tandil, Buenos Aires, Argentina between 12/2000-04/2005. Two pulse field gel electrophoretic patterns predominated. The most frequent one included 5 characteristic isolates of emm1-T1 type, toxin gene profile speA, speB, speF, speG and smeZ. The second pattern included 2 characteristic isolates of emm3-TNT type (speB, speF, speG). The other 3 isolates corresponded to types emm49-TNT (speB, speC, speF, speG), emm75-T25 (speB, speF, speG) and emm83-TNT (speB, speF, speG, ssa, smeZ). All isolates were susceptible to penicillin, cefotaxime, erythromycin, clindamycin, chloramphenicol, tetracycline and rifampicin. The data from the present study demonstrated genetic diversity among the strains. Types emm1 and emm3 were prevalent in invasive disease. The empirical treatment with the combination of penicillin and clindamicin is still valid.

Scientific Electronic Library Online (Spanish)

13

Avances en inmunoterapia celular contra el melanoma maligno/ Advances in cellular immunotherapy for malignant melanoma

López, Mercedes; Escobar, Alejandro; Alfaro, Jorge; Fodor, Miguel; Larrondo, Milton; Ferrada, Carlos; Salazar-Onfray, Flavio
2004-09-01

Resumen en inglés An alternative strategy for cancer treatment is the manipulation of the immune system, denominated cancer immunotherapy. The immunotherapeutical use of cells of the immune system, like dendritic cells (DC), is being explored in different clinical protocols. Recently, we finalized a clinical phase I protocol, for the treatment of malignant melanoma, using DCs loaded with tumor lysates. Our results indicate that the subcutaneous application of DCs do not produce adverse eff (mas) ects. We also observed an increase of tumor spe-cific T lymphocytes precursors in the blood, associated to hypersensitivity reactions (DTH) in 60% of the treated patients. In most cases, an stability in the disease was observed, although without a significant association between vaccination and survival. Additionally, therapies based on Interleukin-2 (IL-2) have been used with relative success in the treatment of some kind of tumors since 1985. However, problems associated to the toxicity of IL-2 still restrict its massive use. Our direct experience with the use of IL-2, indicates that low doses and its subcutaneous application, maintains the beneficial effects for patients, eliminating the adverse effects. Based on the accumulated evidence during last the five years, we decided to implement an optimized clinical protocol, which alternatively combines dendritic cells vaccines with the use of low doses of IL-2 for the reinforcement of the immunological system (Rev Méd Chile 2004; 132: 1115-26)

Scientific Electronic Library Online (Spanish)

14

Devenir de un cambio: del patronato de menores a la protección integral de los derechos de niños, niñas y adolescentes/ Evolution of a change: from the children board to The boys, girls and adolescents integral protection rights

De la Iglesia, Matilde; Velázquez, María Eugenia; Piekarz, Walter
2008-12-01

Resumen en español Considerar las variables socio-históricas respecto de la infancia significa trabajar en pos de los postulados de la Convención Internacional de los Derechos del Niño. A partir de la sanción de la Ley 26.061 de Protección Integral de los Derechos de Niños, Niñas y Adolescentes y la derogación de la Ley 10.903 del Patronato de Menores, en la Argentina se acelera una progresiva transformación en la concepción de la infancia. Pero este cambio se da principalmente en (mas) relación a la legislación y no así en el seno de la comunidad. La modalidad de investigación respondió al paradigma cualitativo, centrada en un especial interés en vislumbrar los procesos sociales desde una perspectiva emic -desde adentro-, desde el lugar en que los propios actores sociales significan dichos procesos. Resumen en inglés To consider the social-historic variables regarding to childhood means to work under the Convention on the Rights of the Child postulates. Since law #26.061 about Integral Protection of childhood Rights approval and law #10.903 about Childhood Boards was abolish in Argentina, a progressive transformation in the childhood conception was accelerated. This transformation is relative to legislation environment but not inside the community. The investigation way respond to the (mas) qualitative paradigm focus in an spe-cial interest in catch a glimpse of social process from an inner perspective, form the scene where the same social actors signify theses processes.

Scientific Electronic Library Online (Spanish)

15

La disolución del suelo en comunidades de Erica andevalensis del entorno de las minas de Riotinto (Huelva, SO España)/ Soil solution in soils under Erica andevalensis formations in the Riotinto mining area (Huelva, SW Spain)

Buján, E.; García-Arrese, A.; Velasco-Molina, M.; Macías, F.
2010-01-01

Resumen en español Erica andevalensis es un brezo endémi­co de la Faja Pirítica Ibérica, catalogado en peligro de extinción por la Junta de Andalucía (Decreto 104/1994, Ley 8/2003), que crece espontáneamente en áreas mineras. El estudio de la disolución de los suelos puede ser una herramienta útil para caracterizar las condiciones geo­químicas de su hábitat. Hemos analizado la disolución del suelo en 11 parcelas de dos áreas mineras de Riotinto. La espe­ciación de iones sol (mas) ubles se estimó me­diante el programa SOLMIN88. Los resultados indican que crece adecua­damente en un amplio rango de condicio­nes, tolerando bien fuerzas iónicas de hasta 10-2 M, disoluciones de reacción hiperácida (3,43) a neutra (6.97) y con concentraciones variables de iones (sulfato 4-376, Ca 0,3-25 y Mg 0,1-25 mg L-1) y metales (Al 0,1-10, Cu 0,01-3,94, Pb 0,01-0,23, Zn 0,01-1,88 y Mn 0,02-2,81 mg L-1). E. andevalensis parece indiferente al catión que domina la disolución del suelo, confirmándose su carácter Al-tolerante. Resumen en inglés Erica andevalensis is an endemic heather in the Iberian Pyrite Belt listed as endan­gered species by Junta de Andalucía (De­cree 104/1994, Law 8/2003) which grows spontaneously in mining areas. The study of soil solution may be a useful tool to charac­terize the geochemical conditions of its habitat. We analyzed the soil solution of 11 plots from two mining areas in Riotinto. Ion speciation was obtained using the SOL­MIN88 software. Our results indicate that it grows (mas) ade­quately under a wide range of conditions, being tolerant to ionic strength until up to 10-2 M, soil solutions ranging from hyper­acid (3.43) to neutral (6.97) and with vari­able ions (sulphate 4-376, Ca 0.3-25 and Mg 0.1-25 mg L-1) and metals (Al 0.1-10, Cu 0.01-3.94, Pb 0.01-0.23, Zn 0.01-1.88 y Mn 0.02-2.81 mg L-1) concentrations. This spe­cies seems to be indifferent to the dominant cation of the soil solution. The Al-tolerant character of this species was also confirmed.

Scientific Electronic Library Online (Spanish)

16

Evidencia microscópica de la presencia de Candida albicans en bases protésicas retiradas de la cavidad bucal/ Microscopic evidence of Candida albicans present in prosthetic bases removed of buccal cavity

Velazco, Gladys; Ortiz, Reynaldo; Arellano, Leylan; Bustillos, Lorena; González, Anajulia
2009-06-01

Resumen en español Se realiza este estudio con el objetivo de demostrar la adherencia de Candida albicans a la ultraestructura de resinas acrílicas de termocurado (PMMA) utilizadas en la confección de las bases de dentaduras totales. Se utilizaron 2 muestras de PMMA: la primera proveniente de bases de dentaduras en uso, para lo cual se seleccionaron 20 pacientes totalmente edéntulos portadores de dentaduras y diagnosticados con estomatitis subprotésica (ESP), y de este grupo se seleccio (mas) nó uno al azar; la segunda muestra proveniente de PMMA recién elaborado bajo el protocolo de formulación tradicional de termocurado. Al observar y comparar ambas muestras en SEM se demostró la presencia de hifas, pseudohifas y clamidosporas en la primera muestra, incluso hifas penetrando hacia defectos de la estructura inherentes al proceso de elaboración. En la segunda muestra hubo una marcada diferenciación topográfica. La evidencia microscópica demostró la adherencia candidiásica en la muestra proveniente de la dentadura en uso. Resumen en inglés Aim of present paper is to determine the Candida albicans adherence to ultrastructure of thermo-cure acrylic resins (PPMA) used in total dentures bases confection. We used 2 samples of PPMA: first from the used dentures bases; choosing 12 completely edentulous patients carriers of dentures and diagnosed with sub-prosthetic stomatitis (SPE) and from this group we choose one at random; the second sample from the PPMA newly developed by the traditional thermo-cure protocol. (mas) Observing and comparing both samples in SEM, it was possible to demonstrate the presence of hyphae, pseudo-hyphae, and chlamydospores in the firs sample even hyphae entering to structure defects inherent to elaboration process. In the second sample there was a marked topographic differentiation. Microscopic evidence showed the Candida adhesion in sample from used dentures.

Scientific Electronic Library Online (Spanish)

17

Detección fenotípica y molecular de resistencia a meticilina en S. aureus/ Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus

Castellano-González, Maribel J; Perozo-Mena, Armindo J; Vivas-Vega, Rosana
2008-06-01

Resumen en español La detección de resistencia a meticilina es complicada debido a la heterogeneidad de su expresión fenotípica; dificultando su detección en el laboratorio, lo que ha conducido al desarrollo de varias técnicas para incrementar su expresión in vitro. A fin de evaluar cuatro técnicas para la detección de resistencia a meticilina: método de difusión del disco con oxacilina (OX, 1 µg) y cefoxitin (FOX, 30 µg); screening test, concentración inhibitoria mínima (CIM) (mas) y detección de PBP2a, utilizando la presencia del gen mecA como método de referencia, se procesaron 286 cepas de S. aureus. Se determinó la sensibilidad (SEN), especificidad (ESP), valor predictivo positivo (VPP), valor predictivo negativo (VPN) y eficiencia (EFC) de cada uno de los métodos. Se obtuvo un total de 50 cepas resistentes a oxacilina, PBP2a positivos (mecA positivos). La sensibilidad del disco de OX fue de 99.14% y la de FOX fue de 100%. La SEN, VPP, VPN y EFC de los otros métodos fue de 100%. Todas, a excepción del método de difusión del disco de OX (ESP de 99.14), resultaron 100% específicos. Resumen en inglés Detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. Four techniques for detecting methicillin resistance were evaluated: the disk diffusion method with oxacillin (OX, 1 µg) and cephoxitin (FOX, 30 µg); the screening test, minimum inhibitory concentration (MIC) and detection of PBP2 (mas) a, using the presence of mecA gen as a reference method; 286 strains of S. aureus, were processed. The sensibility (SEN), specificity (SPE), positive predictive value (PPV), negative predictive value (NPV) and efficiency (EFC) of each method were determined. A total of 50 oxacillin resistant, PBP2a positive (mecA positive) strains were obtained. Sensibility of the OX disk was 99.14%; and of the FOX disk was 100%. The SEN, PPV, NVP and EFC of the other methods were 100%. All the tests, except the OX disk diffusion method (99.14% of ESP), were 100% specific.

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18

Malonildialdehido y peso al nacer en la pre-eclampsia: Breve revisión y asociación entre ambos/ Malondialdehyde and birth weight in preeclampsia: Brief review and association between them

Delgado, Jorge; Reyes, Enrique; Amador, Armando; García, Maura; Cubero, Ormandy; Quesada, Francisco; Veranes Arias, Manuel
2008-12-01

Resumen en español Los trastornos hipertensivos del embarazo (THE), causan frecuente morbilidad y mortalidad perinatal en el mundo y en Cuba y se han vinculado al estrés oxidativo. OBJETIVOS: determinar las características evolutivas de las concentraciones de malonildialdehido en las gestantes sanas (GS) entre las 16-20 semanas (MDA 1) y= 36 semanas (MDA 2), las características de la concentración plasmática de malonildialdehido [MDA] determinadas en las muestras de las preeclámpticas (mas) (MPE) al ingreso, en comparación con las gestantes sanas (MDA 2), así como la relación de [MDA] con el peso al nacer (PN) en preeclámpticas (PE). MÉTODOS: se hallaron las [MDA] en 25 gestantes de acuerdo a criterios de inclusión. Estadísticas: Se determinó medias y desviaciones estándar, comparando resultados de las [MDA] (Mann-Whitney, IC: 94 %, p [MDA] en gestantes sanas (n= 10) (M2). Las [MDA] (MPE) se correlacionaron inversamente con el PN en las preeclámpticas. CONCLUSIONES: la [MDA] se incrementó significativamente (p Resumen en inglés The hypertensive disorders of pregnancy (HDP) cause frequent perinatal morbidity and mortality in the world and in Cuba and they have been linked with oxidative stress. OBJECTIVES: to determine the evolutive characteristics of the concentrations of malondialdehyde in healthy pregnant women between 16 and 20 weeks (MDA 1) and = 36 weeks (MDA 2), the characteristics of the plasmatic concentration of malondialdehyde [MDA] determined in the sample of the preeclamptic women (S (mas) PE) on admission compared with healthy pregnant women (MDA 2), as well as the association of MDA concentrations with birth weight in preeclamptic patients (PE). METHODS: The MDA concentrations were calculated in 25 pregnant women according to inclusion criteria. Statistics: Means and standard deviations were determined by comparing the results of the MDA concentrations (Mann-Whitney, CI: 94 %, p MDA concentrations in healthy pregnant women (n= 10) (M2). The MDA concentrations (SPE) were inversely correlated with birth weight in the preeclamptic patients. CONCLUSIONS: MDA concentrations were significantly higher (p

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19

Adhesivos autograbantes: ¿Grabar o no grabar?/ Self-etching adhesives: To etch or not to etch?

Padrós-Serrat, José Luis; Monterrubio-Berga, Margarita; Padrós-Cruz, Esteban
2003-08-01

Resumen en español Introducción: en el presente estudio se pretende averiguar si podemos aumentar la fuerza de unión a esmalte y a dentina de los adhesivos autograbantes, introduciendo variaciones en la técnica de uso habitual encaminadas a mejorar sus puntos débiles. Es decir, la permanencia de barrillo dentinario, detritus y gérmenes en la cavidad y la teórica debilidad de su unión al esmalte. Material y método: utilizamos el método de los duelos adhesivos con el fin de saber si (mas) un grabado total de la cavidad, o bien un grabado seguido de un lavado con hipoclorito, previos a la aplicación del adhesivo autograbante, aumentarían su eficacia. En la primera parte, dividimos el 1/3 coronario de 120 molares humanos para confrontar las distintas técnicas entre si sobre esmalte en grupos de 10. En la segunda parte dividimos 120 dientes unirradiculares transversalmente unos 2,5 mm por debajo del nivel del esmalte para confrontar los diferentes métodos ensayados sobre la dentina. En la tercera parte tomamos 36 dientes unirradiculares humanos y les tallamos cavidades cervicales por vestibular y por lingual. Los sometimos a tinción con violeta de genciana para valorar la capacidad de filtración marginal. Resultados: las dos modificaciones propuestas mejoran el resultado obtenido tanto en el esmalte como en la dentina, pero sólo significativamente para Prompt-L-Pop y One Step Plus. Las pruebas de filtración acusan permeabilidad del colorante en el esmalte cuando no se graba antes de utilizar el adhesivo, lo que parece recomendar su grabado rutinario. Resumen en inglés Abstract: Introduction: In the present study we try to analyse if we can improve the bond strength and seal of self-etching adhesives to enamel and dentin, by etching with phosphoric acid for 15 seconds or etching followed by deproteinization with sodium hypochlorite 5% for 30 seconds. Material and method: The duel technique is used to compare the different techniques one by one against the others. In the first part the coronal 1/3 of 120 human molars is cut to test bond (mas) strength to enamel. A different technique is used on each side and both parts are bonded with composite resin. By pulling with an Instron machine, we were able to compare which system is stronger in groups of 10. In the second part, 120 human teeth are horizontally cut 2,5mm apical to the enamel margin to test bond strength to dentin. Again a different technique is applied to each side and both sides are bonded with composite resin. In the third part we take 36 human teeth and cervical cavities are prepared in buccal and lingual sides and the teeth are submerged in dye to evaluate leakage. The bonding systems tested were Clearfil SE Bond, Xeno III, One Step Plus + Tyrian SPE and Prompt-L-Pop. Results: Etching with phosphoric acid or etching followed by sodium hypochlorite rinse improve bond strength in enamel and dentin but only significatively for Prompt-L-Pop and One Step Plus. Leakage tests reveal permeability in enamel margins for all self-etching adhesives tested that was not present when cavity was etched, wich seems to indicate that etching of enamel margins when using self-etching bonding systems might be useful. There were no differences between etching or etching followed by sodium hypochlorite rinse before applying the adhesive.

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Analgesia preventiva en mujeres programadaspara esterilización definitiva conelectrofulguración de trompas uterinas porlaparoscopia: Experimento clínico controlado, aleatorizado, con doble enmascaramiento

Navarro, J. Ricardo; Barragán, Gabriel; Rincón, David A; Eslava, Javier H
2008-04-01

Resumen en español Antecedentes: La analgesia preventiva continúa siendo fuente de discusión y desacuerdo entre los especialistas que manejan el dolor. Hay resultados contradictorios, incluidos algunos metaanálisis, sobre la efectividad de la analgesia preventiva con ketamina. Objetivo: Evaluar la eficacia de la ketamina preventiva en la cirugía ambulatoria de mujeres programadas para esterilización definitiva con electrofulguración por laparoscopia, en la disminución del dolor posto (mas) peratorio, comparada contra ketamina administrada de manera no preventiva. materiales y métodos: Entre diciembre de 2007 y enero de 2008 se incluyeron 130 mujeres de la Clínica Piloto de Profamilia, en Bogotá (Colombia), que deseaban planificar mediante ligadura de trompas. Fueron asignadas de manera aleatoria simple a dos grupos: a 71 pacientes se les administró ketamina antes de la incisión quirúrgica (0.25 mg/kg) y a 59 pacientes se les administró ketamina a la misma dosis al fnal del procedimiento. La administración de ketamina y la evaluación de los desenlaces se realizaron de manera enmascarada. Resultados: El nivel de dolor medido mediante la Escala Visual Análoga (EVA) fue similar para ambos grupos, aunque hubo una mayor proporción de pacientes con dolor al minuto 15 en el grupo de ketamina preventiva (19.7% vs. 6.8%, p=0.03). El grupo de ketamina no preventiva presentó más náusea (15% vs 10%) y mareo (46% vs. 37%), aunque esto no fue significativo (p>0,05) Conclusión: La eficacia de la ketamina preventiva para el alivio del dolor postoperatorio no fue diferenre a la de la ketamina no preventiva . Resumen en inglés Background: The pre-emptive analgesia keeps being a source of discussion and discord between the spe-cialists in the pain management. There are contradictory results, even in meta-analysis, about effectiveness of preemptive analgesia with ketamine. Objective: To evaluate effectiveness of ketamine as preemptive analgesia in women under laparoscopic electrofulguration of Falopio tubes, in control of postoperative pain, compared with ketamine administered in a non preemptive (mas) way. material and methods: 130 women who wanted a defnitive contraceptive method as laparoscopic tubal ligation were included with the diagnosis of satisfed parity, from the Clínica Piloto de Profamilia, in Bogotá (Colombia), between december 2007 and january o2008. They were randomly assigned in two groups: 71 received preemptive ketamine (before initiating the surgical procedure, dose of 0,25 mg/kg) and the other 59 patients received ketamine, the same doses, at the end of the procedure. Both, administration of ketamine and evaluation of patients outcomes, were made in blinded form. Results: Patients were similar at the start of the study. The level of pain measured with a visual analog scale (VAS) was similar for both groups, there was a higher proportion of patients with pain in the preemp-tive ketamine group to the minute 15 (29.7% vs 6.8%) Ketamine non preemptive group, felt more nauseous (25% vs. 10% and seasickness (46% vs 37%), this was not signifcative (p>0.05)). Conclusions: The preemptive ketamine wasn't different in effectiveness to ketamine administered in non preemptive way for the relief of postoperative pain .

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DISTRIBUCIÓN BATIMÉTRICA DE FORAMINÍFEROS BENTÓNICOS (PROTOZOA: FORAMINIFERIDA) AL SUR DEL ESTRECHO DE MAGALLANES (52°-56°S), CHILE/ BATHYMETRIC DISTRIBUTION OF BENTHIC FORAMINIFERA (PROTOZOA: FORAMINIFERIDA), SOUTHERN MAGELLAN STRAITS (52°-56°S), CHILE

Hromic, Tatiana
2009-01-01

Resumen en español Se analiza la distribución batimétrica de los foraminíferos bentónicos en el área de canales y fiordos, al sur del estrecho de Magallanes, (52-56°S), Chile. Para ello se estudiaron 34 muestras recolectadas en la zona de plataforma (35 - 650 m), durante la expedición CIMAR 3 FIORDOS (1998), organizada por el Comité Oceanógrafico Nacional (CONA). Se obtuvieron 21.190 ejemplares los que fueron clasificados en 169 especies; sólo el 8,3% de las especies alcanzaron un (mas) a representación superior al 2%, por lo cual, se seleccionaron 47 especies, según su abundancia por tramo, para verificar su distribución. Se reconocieron tres asociaciones: a) de aguas someras (0-150 m); en la porción más superficial (50 m) dominaron Buccella frígida, Buliminella elegantissima y Discorbis berthelothi. Entre 50 y 100 m abundaron Cibicides dispars, Buccella frígida y Ammonia beccarü y en la parte más baja Angulogerina angulosa y Ehrenbergina pupa, b) de aguas intermedias (150 - 350 m); en el estrato más alto de este horizonte (150 - 200 m) dominaron Uvigerina brunnensis, Bulimina notovata y Nonionoides grateloupi. Entre 200 y 250 m se mantuvo la presencia de Nonionoides grateloupi y Bulimina notovata y se hizo presente Oridorsalis tener. Entre 250 y 300 m, se presentaron Angulogerina angulosa, Cassidulina carinata y Globocassidulina rossensis y finalmente, c) de aguas profundas (>350 m) en donde abundaron Oridorsalis tener y Cibicides refulgens, algo más profundamente aún se encontraron Discanomalina vermiculata, Cibicides refulgens y Angulogerina angulosa y finalmente, por debajo de los 600 m se encontraron Angulogerina angulosa, Angulogerina carinata y Cassidulina laevigata Resumen en inglés Bathymetric distribution of benthic foraminifera from the southernmost part of the Chilean chan-nels and fiords (52 - 56°S) was analyzed. Thirty four samples were collected from the Magallanes shelf, south of the Magellan Strait (35 - 650 m). During the Cimar 3 Fiord cruise (1998) organized by the Comité Oceanógrafico Nacional (CONA). 21.190 specimens were collected and classified into 169 spe-cies. Only 8.3% of species had an abundance of more than 2% and 47 species w (mas) ere selected according to their abundance to verify their distribution. Three associations are described: a) shallow water (0-150 m). In the upper layer (0-50 m) BucceUa frigida, Buliminella elegantissima and Discorbis berthelothi were found. Between 50m and 100 m, Cibicides dispars, BucceUa frigida and Ammonia beccarii were abundant and, in the inner part, Anguíogerina angulosa and Ehrenbergina pupa were found. b) Intermedíate water (150 - 350 m); Uvigerina brunnensis, Buíimina notovata and Nonionoides grateíoupi were common between 150 - 200 m. The most abundant species between 200 and 250 m were Nonionoides grateíoupi, Buíimina notovata and Oridorsaíis tener. Anguíogerina angulosa, Cassiduíina carinata and Globocassidulina rossensis were found in the deepest water of this layer (250 y 300 m). c) deep water (>350 m). Oridorsaíis tener and Cibicides refulgens were the main species present and Discanomalina vermiculata, Cibicides refulgens and Anguíogerina angulosa were also found. Anguíogerina angulosa, Anguíogerina carinata and Cassiduíina laevigata were found in the deepest part of the Magellan shelf (below 600 m)

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Contribución al Conocimiento del Síndrome Parapléjico Bovino de  la Ganadería Extensiva de los Llanos de Venezuela. Parte I: Estrategias Diagnósticas/ Contribution to the Knowledge of the Livestock Paraplegic Syndrome  in the Venezuelan Plains. I: Diagnostic Strategies

Marín A, Carlos; de López, Nancy; Marín R, Carlos ; Palencia de Álvarez, Luisa; Sánchez, Antonio; Sandoval, Alberto; de Rolo, Morella; de Veracierta, Coromoto
2006-06-01

Resumen en español Establecimientos ganaderos fundados en ecosistemas de sabanas en los llanos venezolanos desde la Colonia dieron origen a los tradicionales sistemas de producción extensiva-extractiva (SPE), conocidos como vaca-maute y cría-levante, base de explotación de la ganadería de carne en Venezuela. En estos sistemas de producción, los rebaños quedaron expuestos a la adversidad del ambiente durante siglos frente a depredadores, plagas y enfermedades, así como al ciclo lluvia (mas) -sequía, característicos de estas llanuras que abarcan unos 12 millones de hectáreas, donde predominan suelos ácidos y comunidades de pastos del género Trachypogon que sostienen a unos 8 millones de cabezas de mestizo cebú  x criollo. Las condiciones ambientales y de manejo para sostener una ganadería de carne eficiente, no son las más idóneas, debido a escasa innovación tecnológica que es necesario incorporar para mejorar la eficiencia de estos sistemas de producción. Por lo que es necesario considerar que en el origen del Síndrome Parapléjico Bovino (SPB) han estado jugando papel fundamental las condiciones ambientales descritas. Con un modelo que implicó integrar varios niveles de investigación: aplicada, básica y operativa; ésta última realizada en fincas de los productores afectados por el SPB y que fue ejecutada por un equipo multidisciplinario e interinstitucional, se evaluó prioritariamente la situación nutricional y el manejo integral de los animales en la cadena ecológica del sistema-suelo-planta-animal. Los resultados confirmaron que el SPB es un trastorno de origen multifactorial y estrechamente vinculado a deficiencia de minerales, especialmente de fósforo. Resumen en inglés Livestock establishments in the Venezuelan plains that developed in open savannah ecosystems during the time of colony gave origin to the traditional extensive-extractive production systems known as cow-calf and breading-raising, which are the basis for the exploitation of meat livestock in Venezuela. In this type of system, herds were exposed for centuries to adverse environmental conditions, predators, plagues, and diseases as well as to the rainy-draught cycle, charact (mas) eristic of these plains. These regions encompass 12 million hectares, with acid soils where most of their pastures belong to the Trachypogon gender. The pastures sustain about 8 million of crossbred heads (zebu x native). The environmental conditions and the animal’s management that sustain an efficient meat livestock are not the most suitable due to the lack of technological innovations necessary to improve the efficiency of these production systems. It is necessary to consider that the presence of the Bovine Paraplegic Syndrome (BPS) plays a key role in the environmental conditions just mentioned. This study involved several levels of research: applied, basic, and operative. The operative investigation was conducted on farms affected by the BPS and was lead by a multidisciplinary and inter-institutional team. The nutritional situation and the integral management of animals in the soil-plant-animal system were assessed. The results of the investigation confirmed that the BPS is a multifarious closely associated to mineral deficiencies, especially phosphorus.

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Avances y Logros de la Gerencia Sectorial de Diagnóstico y Vigilancia Epidemiológica del Instituto Nacional de Higiene “Rafael Rangel”

Pinto B, Belquis; Albornoz A, María I; López L, Eneida A; Vera, Reviakina; Moros, Rosalba; Guerrero, Leonardo J
2008-06-01

Resumen en español Las actividades de diagnóstico se inician desde el Decreto de creación del Instituto Nacional de Higiene en 1938, donde en el Artículo Nº 2 se establecen las funciones del Instituto, siendo una de ellas la “Investigación sistemática de enfermedades endemoepidémicas y de epizootias nacionales y de todas aquellas enfermedades que puedan existir en el país, y el estudio de los métodos más apropiados para combatirlas”. Es así, como en sus inicios se habla de Labo (mas) ratorios de Bacteriología, Parasitología, Micología, Epidemiología, cuyas actividades funcionaban independientemente bajo la dirección general del Instituto, que actúa como asesor y proveedor de los mismos, perfilándose como un centro de investigación, de consulta y de enseñanza del futuro personal de laboratorio del país, destacándose en estas labores el Dr. Félix Pifano, Dr. Allan Scott, Dr. Martín Mayer, entre otros. La Gerencia Sectorial de Diagnóstico y Vigilancia Epidemiológica ha tenido varias reestructuraciones; actualmente está conformada por la División de Enfermedades Transmisibles, de la cual dependen los Departamentos de Virología, Bacteriología y Micología; y la División de Vigilancia Epidemiológica, a la cual está adscrita la Unidad de Atención al Paciente. La Gerencia ha tenido notable desarrollo, lo cual le ha permitido ser Centro de Referencia Nacional de enfermedades infecciosas en las áreas de Bacteriología, Virología y Micología. Es además un centro de investigación aplicada de enfermedades infectocontagiosas, tales como: Fiebres Hemorrágicas, Encefalitis, Brucelosis, Resistencia Bacteriana, Micosis Superficiales y Profundas y Enfermedades Respiratorias. Contribuye con la Vigilancia Epidemiológica, en apoyo a los programas del Ministerio del Poder Popular para la Salud (MPPS). Se realiza el Diagnóstico y seguimiento de pacientes VIH positivos como parte del Programa Nacional de Control de Infecciones de Transmisión Sexual y SIDA del MPPS. La Gerencia participa activamente en docencia, realizando cursos de Especialización en Micología Médica, pasantías y cursos de pre y postgrados universitarios en las áreas de Microbiología, Infectología y Salud Pública. Coordina y realiza el control de calidad de la Red Nacional de Laboratorios de Salud Pública. Resumen en inglés The diagnostic activities, started with the creation of the National Institute of Hygiene in 1938. due to the fact that N° 2 Article established the Institute functions, being one of them “the systematic investigation of endemoepidemic diseases, and natural epizooties, and all of those illness that might exist all over the country and their appropriate methods to combat them”. That’s why, from the beginning, the bacteriology, parasitological, mycology, epidemiology la (mas) boratories were contemplated, everyonewith different activities, under the Institute General Control, which work as their counselor and supplier coming up as an investigation center, consulting and teaching for the future laboratories workers of the country, being distinguished the doctors Felix Pifano, Scott, Martín Mayer, among others. The Diagnosis Division and Epidemiologic Control Management have got several restructurations. It is currently conformed by the Transmissible Disease Division, from which depend the Virology, Bacteriology and Mycology departments, and the Epidemic Survei llance Division where the Patient Unity Attention is attached. The Diagnostic Management has had a great development, which allowed it to be a National Reference Center of Infectious Diseases, in the bacteriology, virology and mycology fields. It is also a center for applied investigations to infectiouscontagious diseases such, as Bleeding Fever, Brain fever, Brucellosis, Bacterial Resistance, Superficial and Profound Mycosis, and Respiratory diseases. It contributes to the support of epidemiologic alertness, with the Ministerio del Poder Popular para la Salud programs. Diagnosis and follow up of positive VIH patients are carry out, as part of the National Control of Infectious and Sexual Transmission and Aid Programs from the Ministerio del Poder Popular para la Salud. It also participates actively in teaching, with a Medical Mycology spe cialization course, and pre and post academic courses in themi crobiology, infectology and public health fields. It is in charge of Coordination and quality test of the National Public Health La boratories Network.

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Análisis Estratigráfico Secuencial de las Formaciones Huincul y Lisandro del Subgrupo Río Limay (Grupo Neuquén - Cretácico Tardío) en el Departamento El Cuy, Río Negro, Argentina

Sánchez, M. L.; Rossi, J; Morra, S; Armas, P
2008-07-01

Resumen en español Se estudió una sucesión sedimentaria que aflora en el sudeste de la Cuenca Neuquina, en el departamento El Cuy (Río Negro). La misma incluye a las formaciones Huincul y Lisandro que integran el Subgrupo Río Limay (Grupo Neuquén), y han sido asignadas al Cenomaniano- Turoniano. Se identificaron once litofacies de origen fluvial, tres litofacies de naturaleza eólica y dos litofacies volcaniclásticas. A partir de los agrupamientos en unidades genéticamente relacionad (mas) as se definieron siete elementos fluviales de intracanal, cuatro de planicie de inundación y cuatro eólicos. La distribución espacial y relaciones entre los elementos arquitecturales ha permitido reconocer sistemas fluviales agradantes de alta sinuosidad (SF-I y II), un sistema de abanico terminal (SAT) y campos de médanos barjanoides (SE). El análisis estratigráfico-secuencial permitió definir cinco Secuencias a partir de las relaciones espaciales y temporales de los elementos arquitecturales mayores y de la determinación de superficies estratigráficamente significativas. La base de la Formación Huincul está representada por el SF-I, caracterizado por un sistema de alta sinuosidad, con baja relación canales/planicie de inundación y abundantes depósitos de desbordes. Este tramo de la secuencia se interpreta como el Cortejo de Alta Acomodación (AA) de la Secuencia I (S-I). El sector cuspidal de la unidad está integrado por un sistema similar pero con una alta relación canal/planicie de inundación y constituye el Cortejo de Baja Acomodación (BA) de la Secuencia II (S-II). Se propone que la depositación de la Formación Huincul tuvo lugar bajo condiciones climáticas cálidas con un régimen de estacionalidad marcado. La Formación Lisandro se inicia con depósitos de un sistema distributario proximal de abanico terminal (SAT) que representan el Cortejo AA de S-II. De aquí en adelante es notoria la recurrencia de SE, caracterizando los Cortejos BA y una sucesión desde cuenca de inundación a distributarios proximales de SAT, integrando los Cortejos AA de las secuencias S-II, III, IV y V. La unidad se depositó bajo condiciones climáticas semiáridas permanentes. Las unidades estratigráficas estudiadas se consideran de carácter sinorogénico, con un control tectónico significativo en la definición del espacio de acomodación y un volcanismo principalmente activo durante la sedimentación de la Formación Huincul. La Formación Lisandro corresponde a un período de abrupto incremento en la tasa de subsidencia en la Cuenca Neuquina. Resumen en inglés In the Cuy Department, Rio Negro Province (Fig. 1), there are outcrops of the Late Cretaceous continental deposits of the Subgroup Rio Limay (Fig. 2), in virtually continuous exposures that allow detail studies and exploration of the architecture of the sedimentary bodies. This contribution presents the results of the sedimentological study and sequential stratigraphic analysis for the depositional period of the Huincul and Lisandro formations (Subgroup Río Limay). The o (mas) utcrops appear in the Planicie de Rentaría (Area A), with continuous sections longer than 3 km and Anfiteatro (Area B) with outcrops of more than 5 km (Fig. 1). In these areas we made detailed sedimentological profiles (Fig. 3), we described and interpreted lithofacies and architectural elements employing photograms and we defined stratigraphicaly significant discontinuities. We identified 11 fluvial lithofacies, 4 conglomerates (Gm, Gh, Gp and Gt), 5 sandstones (Sm, St., Sp, and Mr. Sh), and 2 pelitic (Fl and Fm), according to the nomenclature of Miall (1996). In addition, 2 volcaniclastic lithofacies, 1 primary pyroclastic lithofacies (Tlpm) and 1 volcaniclastic sineruptive lithofacies (TLpe) following criteria Mc Phie et al. (1993) were defined, and finally 3 eolic lithofacies (Spe, She and Sathya) (Fig. 4). These were grouped into 11 fluvial architectural elements and 4 eolic (Fig. 5). We have recognized channel elements (CHI) and CHII), intrachannel (LA, DAI, DAII, SBI and SBII) and floodplain (CRI, CRII, CS and OF). The eolic architectural elements include dunes (ED) and interdunes (DI, and WI FI). The paleoenvironments sedimentary include river systems, terminal fans and dune fields. The Sistema Fluvial I (SF-I) is integrated by LA, DAI, DAII, SBI, CHI, CRI, CRII and OF (Fig. 6). The meanders loops show, in most cases, complete filling sequences in which records of migration of large sand dunes are preserved which retain topsets deposits and the scrolls their undulating tops. The channel belts are separated by potent floodplain deposits, with frequent intercalation of levels of overflow both channelized or in mantle, and show a multilateral persistence in potent sections of the column. The crevasse splay exhibits paleosoils with low levels of development, frequent presence of gleyzed horizons and high degree of bioturbation. The general characteristics of the S-FI are proper of aggrading fluvial systems of high sinuosity. The Sistema Fluvial II (SF-II) is characterized by LA, DAI, DAII, SBI and to a lesser proportion, by CRI and OF (Fig. 6). The meanders loops often show preservation of the channel fill by lateral accretion with complete successions. Also the dunes preserve topsets deposits and the scrolls the original topography (Fig. 7). The frequency of conservation of filling phase of the abandoned channel with strong bioturbation and development gleyzed horizons suggests frequent avulsion by strangulation. The preservation of the floodplain is low, and it is characterized by intense bioturbation, and a high volcaniclastic participation in some reaches. We consider that the fluvial system model is of high sinuosity highly aggrading. The terminal fan system (Sistema Abanico Terminal) (TSS), characteristic of semiarid regions with strong seasonal climatology, is represented by CHII, CS or SBII and OF (Fig. 6). These constitute flooding basin deposits (CI), simple multiepisodic channel fillings corresponding to middle sections of the distributary plain and simple channels with development of small transversal cross bars and multilateral relations of distal distributary plain. The aeolian system (Sistema Eolico) (IS) consists of SD, DI, WD and FD. The spatial distribution of dunes and interdunes, the high dispersion of paleocurrents with a principal mode at the avalanche deposits and multiple stabilization surfaces suggest the development of a field of barchan dunes. In the element WI, we identified ferric and mottled halos and bioturbations that indicate the presence of paleosoils with gleyzed horizons (Retallack, 1976, 1990) which and characterize environments with good oxygenation. According to the temporal and spatial arrangement of the different paleoenvironments, their evolutionary trend and the analysis of significant discontinuity surfaces, we produced the stratigraphic sequence for the Subgroup Rio Limay (Fig. 8). The sequence starts with the Secuencia I, corresponding to the base of the Huincul Fm and is represented by SF-I, defined by belts of potent channels, isolated in floodplain deposits and with the development of overflow fans under conditions of rapid aggradation. The avulsion by overflow crevasse splay was the control mechanism of the system. The development of composite paleosoils in distal segments of the plain indicates the existence of long periods during which the channel band remained relatively stable poitions. The incorporation of volcaniclastic materials to fluvial deposits claims a volcanic activity close in time. The geometry and continuity of the sandy bodies and its proportion in relation to the floodplain deposits suggests periods of low frequency of avulsion/subsidence rate. The large-scale architecture observed is the equivalent to a High Accommodation System Track (Cortejo) (AA) with a ratio A/S close to 1 associated with a gradual decline in the base level under conditions of high sediment supply. A net surface (Discontinuidad D-IS-IS) is the basis of the sequence II, marked by an abrupt change in the fluvial architecture river (SF-II). The mechanism of lateral migration was by strangulation and abrupt cut of the bends. Only underdeveloped paleosoils in the abandoned channel fillings and the little preservation of the floodplain deposits are recognized. The stacking of channel belts signs out a reduction in the accommodation space. The top layer of Huincul Fm is interpreted as Lower Accommodation System Track (Cortejo) (BA). The Discontinuity IIC (D-IIC) marks the base of Lisandro Fm, which is an surface of fluvial flooding defining the expansion of a terminal fan system associated with an abrupt increase in the accommodation space and rapid progradation of the facies of an environment of intermediate distributary plain. This stretch of the Sequence is interpreted as the High Accommodation System Track (Cortejo) (AA) of the Sequence II (S-II) was associated with a period of high subsidence. The deposits of terminal fans are covered by a wet eolian system (IS). The change in the depositional environment marks the beginning of the Sequence III (SIII), limited at the base by D-IIIS. The development of a field of parabolic dunes associated with environments dry, wet and flooded interdune indicates a gradual rise in the water table that, eventually, was above the depositional surface generating shallow lagoons. The shortterm climatic changes related with an increase in precipitation and the relative position of the water table controlled the development stabilization surfaces and the growth and migration of aeolian forms. We identified two supersurfaces (D-IV and DV, Fig. 18) which may be assigned to periods with an increase in the supply or availability of sediment climatically conditioned. Periodically, the interdune corridors were overrun by ephemeral streams associated with the flood basin environments of SAT during flooding events. All deposits, characterized by a strong stacking of the eolian successions are interpreted as the Cortejo BA from the Sequence III. A new fluvial flood event marked by the Discontinuity VIC, is represented by facies flood basin, a distributary plain with the development of intertwined channels showing evidences of the SAT progradation. This sector is interpreted as the (Cortejo) AA System Track of S-III associated with a period of renewed subsidence. D-VIIS limits the base of the S-IV whose (Cortejo) BA System Track is similar to the previous Sequence and includes 3 supersurfaces (D-D-VIIIIX / X). The expansion of the flooding basin facies of a limited terminal fan at the base by D-XIC represents the base of the (Cortejo) AA System Track of the Sequence IV. Tabular units associated with a distal distributary plain that replaced towards the top by thick channels of low sinuosity close to the backbone of the system represent an progradation event of the SAT. The beginning of the Sequence V is marked D-XIIS associated with the development of a new SE. The architectural design of large scale is similar to that of the (Cortejos) BA System Tracks of the Sequences III and IV and includes supersurfaces D-D-XIII and XIV. Something similar occurs with the surface of fluvial flooding (D-XVC) on which we identified a prograding succession of SAT. The characteristics of the systems involved in the Lisandro Fm suggest a climate change to semi-arid conditions and low gradient in the depositional environment. The sequential stratigraphic analysis of the formations Huincul and Lisandro suggests that the deposits are sinorogénic and the different pulses of subsidence are registered in the (Cortejos) AA and BA System Tracks defined in this study.

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Validation of an enzyme-linked immunosorbent assay (ELISA) for the determination of 4-nonylphenol and octylphenol in surface water samples by LC-ESI-MS

Céspedes, Raquel; Skryjová, K.; Raková, M.; Zeravik, J.; Fránek, M.; Lacorte Bruguera, Silvia; Barceló, Damià
2006-11-15

Digital.CSIC (Spain)

26

Tracing Pharmaceutical Residues of Different Therapeutic Classes in Environmental Waters by Using Liquid Chromatography/Quadrupole-Linear Ion Trap Mass Spectrometry and Automated Library Searching

Gros, Meritxell; Petrovic, Mira; Barceló, Damià

15 pages, 6 figures.-- Article in press. | This article describes the development, optimization, and validation of an analytical method for the simultaneous detection and identification of 73 pharmaceutical residues, covering various therapeutic groups, in both surface and wastewaters. The method is...

DRIVER (Spanish)

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Simplified procedures for the analysis of polycyclic aromatic hydrocarbons in water, sediments and mussels

Martínez, Elena; Gros, Meritxell; Lacorte Bruguera, Silvia; Barceló, Damià
2004-08-11

Digital.CSIC (Spain)

33

Septum-Localized Protein Required for Filament Integrity and Diazotrophy in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120

Flores, Enrique; Pernil, Rafael; Muro-Pastor, Alicia M.; Mariscal, Vicente; Maldener, Iris; Lechno-Yossef, Sigal

Journal of Bacteriology Vol. 189(10) p.3884-3890 | Heterocysts, formed when filamentous cyanobacteria, such as Anabaena sp. strain PCC 7120, are grown in the absence of combined nitrogen, are cells that are specialized in fixing atmospheric nitrogen (N2) under oxic conditions and that transfer fixed...

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Sensibilidad antimicrobiana y caracterización de cepas de Streptococcus pyogenes aisladas de un brote de escarlatina/ Antimicrobial sensitivity and typing of Streptococcus pyogenes strains isolated during a scarlet fever outbreak

Pedraza-Avilés, Alberto González; Ortiz-Zaragoza, Catalina; Mota-Vázquez, Ricardo; Dickinson-Bannack, Ma Eloísa; Dávila-Mendoza, Rocío; Fernández-Ortega, Miguel Angel
2002-09-01

Resumen en español Objetivo. Evaluar la actividad in vitro de 13 antibióticos contra 47 Streptococcus pyogenes grupo A (SGA). Determinar la presencia de genes que codifican para exotoxina pirogénica estreptocóccica A (SpeA) y serotipos con base en proteína M. Material y métodos. Estudio transversal hecho en el Centro de Salud Dr. José Castro Villagrana sobre un brote de escarlatina en el Colegio Espíritu de América, entre diciembre de 1999 y enero de 2000. El número de niños estud (mas) iados fue 137. Se extrajeron porcentajes de sensibilidad. La concentración inhibitoria mínima (CIM) se obtuvo por microdilución semiautomatizada. Se utilizó un secuenciador automatizado de DNA para el análisis de variación de secuencias en los genes que codifican para proteína M y SpeA. Resultados. Todas las cepas fueron sensibles a beta-lactámicos y clindamicina; 12.7% fueron resistentes a eritromicina. El serotipo M2 fue el más frecuente, 27 del total. Prácticamente todas las bacterias (96%) con el gen SpeA tienen el gen que codifica para el serotipo M2. Conclusiones. Debido a la reciente reaparición de infecciones por SGA se sugiere realizar estudios tanto de sensibilidad a macrólidos y beta-lactámicos, como de epidemiología molecular. Resumen en inglés Objective. To evaluate the in vitro activities of 13 antimicrobial agents against 47 group A Streptococcus pyogenes (GAS) strains, and to determine the presence of genes encoding streptococcal pyrogenic exotoxin A (SpeA) and the M--protein serotypes. Materials and Methods. A cross-sectional study was conducted at Centro de Salud Dr. José Castro Villagrana, during a scarlet fever outbreak occurring between December 1999 and January 2000, among 137 children at Colegio Esp� (mas) �ritu de América. Minimum Inhibitory Concentrations (MICs) were obtained by the semiautomated microdilution method. Automated DNA sequencing was used for analysis of sequence variation in genes encoding the M protein, and SpeA. Results. All strains were sensitive to betalactams and clindamycin. Six (12.7%) were resistant to erythromycin. The M2 type was the most frequently isolated GAS (27); almost all (96%) bacteria with the SpeA gene had the gene encoding the M2 protein. Conclusions. The recent resurgence of GAS infections calls for molecular epidemiology research and studies on the sensitivity to macrolides and beta-lactams.

Scientific Electronic Library Online (Spanish)

35

Sensibilidad antimicrobiana y caracterización de cepas de Streptococcus pyogenes aisladas de un brote de escarlatina/ Antimicrobial sensitivity and typing of Streptococcus pyogenes strains isolated during a scarlet fever outbreak

Pedraza-Avilés, Alberto González; Ortiz-Zaragoza, Catalina; Mota-Vázquez, Ricardo; Dickinson-Bannack, Ma Eloísa; Dávila-Mendoza, Rocío; Fernández-Ortega, Miguel Angel
2002-09-01

Resumen en español Objetivo. Evaluar la actividad in vitro de 13 antibióticos contra 47 Streptococcus pyogenes grupo A (SGA). Determinar la presencia de genes que codifican para exotoxina pirogénica estreptocóccica A (SpeA) y serotipos con base en proteína M. Material y métodos. Estudio transversal hecho en el Centro de Salud Dr. José Castro Villagrana sobre un brote de escarlatina en el Colegio Espíritu de América, entre diciembre de 1999 y enero de 2000. El número de niños estud (mas) iados fue 137. Se extrajeron porcentajes de sensibilidad. La concentración inhibitoria mínima (CIM) se obtuvo por microdilución semiautomatizada. Se utilizó un secuenciador automatizado de DNA para el análisis de variación de secuencias en los genes que codifican para proteína M y SpeA. Resultados. Todas las cepas fueron sensibles a beta-lactámicos y clindamicina; 12.7% fueron resistentes a eritromicina. El serotipo M2 fue el más frecuente, 27 del total. Prácticamente todas las bacterias (96%) con el gen SpeA tienen el gen que codifica para el serotipo M2. Conclusiones. Debido a la reciente reaparición de infecciones por SGA se sugiere realizar estudios tanto de sensibilidad a macrólidos y beta-lactámicos, como de epidemiología molecular. Resumen en inglés Objective. To evaluate the in vitro activities of 13 antimicrobial agents against 47 group A Streptococcus pyogenes (GAS) strains, and to determine the presence of genes encoding streptococcal pyrogenic exotoxin A (SpeA) and the M--protein serotypes. Materials and Methods. A cross-sectional study was conducted at Centro de Salud Dr. José Castro Villagrana, during a scarlet fever outbreak occurring between December 1999 and January 2000, among 137 children at Colegio Esp� (mas) �ritu de América. Minimum Inhibitory Concentrations (MICs) were obtained by the semiautomated microdilution method. Automated DNA sequencing was used for analysis of sequence variation in genes encoding the M protein, and SpeA. Results. All strains were sensitive to betalactams and clindamycin. Six (12.7%) were resistant to erythromycin. The M2 type was the most frequently isolated GAS (27); almost all (96%) bacteria with the SpeA gene had the gene encoding the M2 protein. Conclusions. The recent resurgence of GAS infections calls for molecular epidemiology research and studies on the sensitivity to macrolides and beta-lactams.

Scientific Electronic Library Online (Spanish)

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Seasonal distribution and spawning of small tunas (Auxis rochei and Sarda sarda) in the Northwestern Mediterranean

Sabatés, Ana; Recasens, Laura

Publicación online disponible en: http://www.icm.csic.es/scimar/index.php | Bullet tuna, Auxis rochei and Atlantic bonito, Sarda sarda are the most abundant small tuna species in the Mediterranean Sea. Both species are commercially exploited by a small-scale inshore fishery off the Catalan coast. Ca...

DRIVER (Spanish)

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Recently developed GC/MS and LC/MS methods for determining NSAIDs in water samples

Farré Urgell, Marinel.la; Petrovic, Mira; Barceló, Damià
2007-01-04

Digital.CSIC (Spain)

39

Recent advances in LC-MS residue analysis of veterinary medicines in the terrestrial environment

Díaz-Cruz, M. Silvia; Barceló, Damià

10 pages, 2 figures, 3 tables.-- Printed version published Jun 2007.-- Issue title: Pharmaceutical-residue analysis. | Despite some European countries having banned the use of veterinary medicines for non-therapeutic purposes in food-animal agriculture, large amounts of pharmaceutical compounds, mos...

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Pilot survey monitoring pharmaceuticals and related compounds in a sewage treatment plant located on the Mediterranean coast

Gómez, M. José; Martínez Bueno, M. J.; Lacorte Bruguera, Silvia; Fernández Alba, Amadeo R.; Agüera, A.
2007-01-01

Digital.CSIC (Spain)

46

Phenolic profiles of Portuguese olive fruits (Olea europaea L.): Influences of cultivar and geographical origin

Ferreres, Federico; Vinha, Ana F.; Silva, Branca M.; Valentão, Patricia; Gonçalves, Ana; Pereira, José Alberto; Oliveira, M. Beatriz; Seabra, Rosa M.; Andrade, Paula B.
2005-03-01

Digital.CSIC (Spain)

47

Part per trillion determination of atrazine in natural water samples by a surface plasmon resonance immunosensor

Farré Urgell, Marinel.la; Martínez, Elena; Ramón-Azcón, Javier; Navarro Ortega, Alicia; Radjenovic, Jelena; Mauriz, Elba; Lechuga, Laura M.; Marco, María Pilar; Barceló, Damià
2007-03-13

Digital.CSIC (Spain)

48

Organic compounds in paper-mill process waters and effluents

Lacorte Bruguera, Silvia; Latorre Fernández, Anna; Barceló, Damià; Rigol, Anna; Malmqvist, Asa; Welander, Thomas
2003-11-01

Digital.CSIC (Spain)

49

Optical spectroscopic study of Eu(3+) crystal field sites in Na3La9O3(BO3)8 crystal

Cascales Sedano, M.ª Concepción; Balda, Rolindes; Jubera, V.; Chaminade, J. P.; Fernández Rodríguez, Joaquín

10 pages, 4 figures.-- OCIS codes: 140.3380, 300.6320.-- PMID: 18542349 [PubMed].-- Printed version published on Feb 18, 2008.-- © 2008 Optical Society of America. | Time-resolved line-narrowed fluorescence spectroscopy of Eu(3+) ions in a new oxyborate Na3La9O3(BO3)8 crystal shows the existence of ...

DRIVER (Spanish)

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Occurrence of Nonylphenol and Bisphenol-A in Surface Waters from Portugal

Azevedo, Débora de A.; Lacorte Bruguera, Silvia; Viana, Paula; Barceló, Damià
2001-01-01

Digital.CSIC (Spain)

51

Nuevas metodologías de análisis de pesticidas por electroforesis capilar

García Montelongo, Francisco Javier; Rodríguez Delgado, Miguel A.; Hernández Borges, Javier
2006-01-01

Digital.CSIC (Spain)

52

Notch1 and IL-7 Receptor Interplay Maintains Proliferation of Human Thymic Progenitors while Suppressing Non-T Cell Fates

García-Peydró, Marina; Yébenes, Virgina de; Toribio, Maria Luisa

Article available at http://www.jimmunol.org/cgi/content/abstract/177/6/3711 | Notch signaling is critical for T cell development of multipotent hemopoietic progenitors. Yet, how Notch regulates T cell fate specification during early thymopoiesis remains unclear. In this study, we have identified an...

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Integrated procedure for determination of endocrine-disrupting activity in surface waters and sediments by use of the biological technique recombinant yeast assay and chemical analysis by LC-ESI-MS

Céspedes, Raquel; Petrovic, Mira; Raldúa, Demetrio; Saura, Úrsula; Piña, Benjamín; Lacorte Bruguera, Silvia; Viana, Paula; Barceló, Damià
2004-02-04

Digital.CSIC (Spain)

57

Inelastic collisions in para-H2: Translation-rotation state-to-state rate coefficients and cross sections at low temperature and energy

Maté, Belén; Thibault, Franck; Tejeda, Guzmán; Fernández Sánchez, José María; Montero, Salvador

8 pages, 5 figures.-- PACS nrs.: 34.50 Ez. | We report an experimental determination of the k(00-->02) rate coefficient for inelastic H2:H2 collisions in the temperature range from 2 to 110 K based on Raman spectroscopy data in supersonic expansions of para-H2. For this purpose a more accurate metho...

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59

How Athila retrotransposons survive in the Arabidopsis genome

Marco, Antonio; Marín, Ignacio

This article is available from: http://www.biomedcentral.com/1471-2164/9/219 | [Background] Transposable elements are selfish genetic sequences which only occasionally provide useful functions to their host species. In addition, models of mobile element evolution assume a second type of selfishness:...

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Hacia el desarrollo de técnicas analíticas rápidas para la detección de tóxicos y contaminantes en alimentos

Ramos, Lourdes; González, María J.; Fontecha, Javier

La OTRI del Centro Tecnológico Nacional de la Conserva y Alimentación junto con la OTT del Consejo Superior de Investigaciones Científicas, colaboran en el Proyecto AGROCSIC, el cual fue aprobado por el Ministerio de Ciencia y Tecnología y financiado por el Ministerio de Educación y Ciencia. El obje...

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Fully Automated Analysis of beta-Lactams in Bovine Milk by Online Solid Phase Extraction-Liquid Chromatography-Electrospray-Tandem Mass Spectrometry

Kantiani, Lina; Farré Urgell, Marinel.la; Sibum, Martin; Postigo, Cristina; López de Alda, Miren; Barceló, Damià
2009-04-29

Digital.CSIC (Spain)

66

Fast solid-phase extraction-gas chromatography-mass spectrometry procedure for oil fingerprinting. Application to the Prestige oil spill

Alzaga Morales, Roberto; Montuori, Paolo; Ortiz Vera, Laura; Bayona Termens, Josep María; Albaigés Riera, Joan
2004-01-30

Digital.CSIC (Spain)

70

Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)

Farré Urgell, Marinel.la; Brix, Rikke; Kuster, Marina; Rubio, Fernando; Goda, Yasuhiro; López de Alda, Miren; Barceló, Damià
2006-07-01

Digital.CSIC (Spain)

71

Evaluation of a newly developed enzyme-linked immunosorbent assay for determination of linear alkyl benzenesulfonates in wastewater treatment plants

Farré Urgell, Marinel.la; Ramón-Azcón, Javier; Galvé, Roger; Marco, María Pilar; Barceló, Damià
2006-07-19

Digital.CSIC (Spain)

72

Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados/ Molecular epidemiology of a Streptococcus pyogenes related nosocomial outbreak in a burn unit

Fica C, Alberto; Fernández O, Jorge; Ebensperger D, Germán; Cona T, Erna; Galanti D, Andrea; Alonso M, Catalina; Ulloa F, María Teresa; Frola M, Ana María; Prat M, Soledad
2003-02-01

Resumen en inglés Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to another child in the emergency room before he was transferred to the burn unit. Patients developed burn infections or invasive disease. One of them died due to a toxic shock syndrome (mas) and 3 other lost their skin grafts. Perineal and nasal microbiological surveillance of 42 related health care workers identified only one of them as carrier of S pyogenes. Aim: To report a molecular analysis of an apparently clonal outbreak. Material and methods: The available isolates were analyzed by molecular methods including random amplified polymorphic DNA analysis (RAPD) with 4 different primers, Sma-I pulsed field gel electrophoresis (PFGE) analysis, and speA, speB and speC detection by polymerase chain reaction (PCR). Results: Two phylogenetically distant and sequentially isolated bacterial groups were identified either by RAPD analysis with selected primers or by Smal-PFGE analysis. The first group involved isolates identified in two patients that included the lethal case. The second bacterial group comprised 5 clinical isolates and the perineal and nasal isolates obtained from a health care worker. Only strains belonging to the first group harbored the speA gene and were associated with invasive disease. The second group could be split further in two subgroups according to their speB profile. Conclusions: RAPD analysis with selected primers can reproduce the PFGE-discriminating ability on the epidemiological analysis of GAS infections (Rev Méd Chile 2003; 131: 145-54)

Scientific Electronic Library Online (Spanish)

73

Effects of irregular shape and topography in the thermophysical models of heterogeneous cometary nuclei

Gutiérrez, Pedro J.; Ortiz, J. L.; Rodrigo Montero, Rafael; López-Moreno, J. J.

The final version of the paper is available at: http://dx.doi.org/10.1051/0004-6361:20010742 | Several improvements in the thermophysical model by Gutierrez et al. (2000) have been included in a new code to specifcally deal with fully irregular cometary nuclei. Also, the new code allows for the incl...

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76

Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

Pérez Solsona, Sandra; McJury, Brenna E.; Eichhorn, Peter; Aga, Diana S.
2007-10-24

Digital.CSIC (Spain)

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Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica/ Detection of pyrogenic exotoxin SpeA, SpeB and SpeC genes in Chilean streptococci isolates and their association with clinical manifestations

Ulloa F, María Teresa; Giglio M, María Soledad; Porte T, Lorena; Santa Cruz A, Adriana; McNab M, Paul; Fica C, Alberto; Pinto C, María Eugenia; Kawaguchi G, Keyko; Carmi K, Alejandra
2000-01-01

Resumen en inglés Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Streptococcus pyogenes strains and their association with the invasiveness of infections. Material and methods: The genes for pyrogenic exotoxins SpeA, SpeB and SpeC were determined (mas) by polymerase chain reactions in 114 strains of group A Streptococcus pyogenes isolated from Chilean patients with invasive or non invasive infections. Results: The gene for SpeA was present in 30.7% of isolates, the gene for SpeB was present in 69.3% and the gen for SpeC in 44.7% of isolates. The gene for SpeA was present in 20 of 33 invasive infections and in 15 of 81 non invasive infections (p <0.0001). On the contrary, the gene for SpeC was present in 11 of 33 invasive infections and in 41 of 81 non invasive infections (p <0.05). The frequency of speB was similar in invasive and non invasive infections. Conclusions: There is a clear relationship between the presence of SpeA genes and the severity of infections caused by Streptococcus pyogenes. (Rev Méd Chile 2000; 128: 27-34)

Scientific Electronic Library Online (Spanish)

85

Deceptive plumage signals in birds: manipulation of predators or prey?

Negro Balmaseda, Juan José; Bortolotti, Gary R.; Sarasola, José Hernán
2007-01-01

Digital.CSIC (Spain)

87

Chemical characterization of the lipophilic fraction of Giant reed (Arundo donax) fibres used for pulp and paper manufacturing

Coelho, Dora; Marques, Gisela; Gutiérrez Suárez, Ana; Silvestre, Armando R.D.; Río Andrade, José Carlos del
2008-01-01

Digital.CSIC (Spain)

88

Biomonitoring Human Exposure to Organohalogenated Substances by Measuring Urinary Chlorophenols Using a High-Throughput Screening (HTS) Immunochemical Method

Nichkova, Mikaela; Marco, María Pilar

9 pages, 5 tables, 3 figures. | The widespread contamination of the environment by persistent organochlorinated substances is well-known. High-throughput immunochemical methods may improve routine assessment of the exposure of the population to these chemicals by analyzing urinary biomarkers. Trichl...

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Biodegradation studies of selected priority acidic pesticides and diclofenac in different bioreactors

González Blanco, Susana; Müller, Jutta; Petrovic, Mira; Barceló, Damià; Knepper, Thomas P.
2006-12-01

Digital.CSIC (Spain)

91

Benzoxazinoid Allelochemicals in Wheat: Distribution among Foliage, Roots, and Seeds

Villagrasa, Marta; Guillamón, Miriam; Labandeira Robés, Ana Mª; Taberner, Andreu; Eljarrat, Ethel; Barceló, Damià
2006-06-24

Digital.CSIC (Spain)

92

BARRUFETA BRAVENSIS GEN. NOV. SP. NOV. (DINOPHYCEAE): A NEW BLOOM-FORMING SPECIES FROM THE NW MEDITERRANEAN SE

Sampedro N., Fraga S.; Penna A., Casabianca,S.; Zapata, Fuentes Grünewald C. M.; Riobó P., Camp J.
2011-01-01

Digital.CSIC (Spain)

95

Análisis multicaracter para estimar parámetros genéticos para crecimiento en bovinos de raza Braford en Corrientes

López, María V.; Arias Mañotti, Adolfo A.; Slobodzian, Ana
2007-06-01

Resumen en español Se ha efectuado un estudio en La Estación Experimental Agropecuaria Corrientes del INTA sobre un rodeo Braford estabilizado 3/8 Brahman x 5/8 Hereford, que tiene como propósito estimar los parámetros genéticos y ambientales para peso al nacer (PNAC), peso ajustado a 210 días (P_AJ_210) y peso ajustado a 570 días (P_AJ_570), mediante el Modelo Animal Multicaracter, utilizando el software MTDFREML. Se procesó una base de datos de 658 registros de producción y geneal (mas) ogía de bovinos de raza Braford, correspondientes al período 1992-2004. Los componentes de varianza y covarianza, y parámetros genéticos y ambientales estimados son: a) Para PNAC: s²a = 6,57, s²m = 2,21, s am = -0,32, s²pe = 0,72, s²e = 7,31, s²p = 16,50, h²a = 0,40, h²m = 0,13, r am = -0,09, c² = 0,04, e² = 0,44; b) Para P_AJ_210: s²a = 153,89, s²m = 62,18, s am = -56,47, s²pe = 10,35, s²e = 223,05, s²p = 393,02, h²a = 0,39, h²m = 0,16, r am = -0,58, c² = 0,02, e² = 0,57; c) Para P_AJ_570: s²a = 292,95, s²e = 419,02, s²p = 711,97, h²a = 0,41, e² = 0,59. Además se calcularon las correlaciones genéticas entre los caracteres analizados: PNAC-P_AJ_210: 0,83, PNAC-P_AJ_570: 0,31, P_AJ_210-P_AJ_570: 0,74. Finalmente se obtuvieron los valores genéticos de los reproductores (DEPs), cuya combinación apropiada permitirá tomar decisiones de selección para el mejoramiento de esta raza. Resumen en inglés This study was carried out at INTA Corrientes Experimental Station with data from a stabilized Braford 3/8 Brahman x 5/8 Hereford herd. The objective was to estimate the genetic and environmental parameters for birth weight (PNAC), weight adjusted to 210 days of age (P_AJ_210), and weight adjusted to 570 days of age (P_AJ_570). We used a Multivariate Animal Model and MTDFREML software. A database of growth and genealogy data from 658 samples of Braford males and females c (mas) orresponding to the period 1992-2004 was processed. Estimates of variance and covariance components and the genetic and environmental parameters were: a) For PNAC: s²a = 6,57, s² m = 2,21, s am = -0,32, s²pe = 0,72, s²e = 7,31, s²p = 16,50, h²a = 0,40, h²m = 0,13, r am = -0,09, c² = 0,04, e² = 0,44; b) For P_AJ_210: s²a = 153,89, s²m = 62,18, s am = -56,47, s²pe = 10,35, s²e = 223,05, s²p = 393,02, h²a = 0,39, h²m = 0,16, r am = -0,58, c² = 0,02, e² = 0,57; c) For P_AJ_570: s²a = 292,95, s²e = 419,02, s²p = 711,97, h²a = 0,41, e² = 0,59. Genetic correlations among the characters analyzed were also calculated: PNAC-P_AJ_210: 0,83, PNAC-P_AJ_570: 0,31, P_AJ_210-P_AJ_570: 0,74. Finally, the genetic value of each individual was obtained (Expected Progeny Differences - EPDs). The combination of these values will allow to improve the characteristics of this breed.

Scientific Electronic Library Online (Spanish)

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Analysis of pesticides in soy milk combining solid-phase extraction and capillary electrophoresis-mass spectrometry

Hernández-Borges, Javier; Rodríguez-Delgado, Miguel Ángel; García-Montelongo, Francisco J.; Cifuentes, Alejandro

In this work, the determination of a group of triazolopyrimidine sulfoanilide herbicides (cloransulam-methyl, metosulam, flumetsulam, florasulam and diclosulam) in soy milk by capillary electrophoresis-mass spectrometry (CE-MS) is presented. The main electrospray interface (ESI) parameters (nebulize...

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Analysis of pesticides in soy milk combining solid-phase extraction and capillary electrophoresis-mass spectrometry

Hernández Borges, Javier; Rodríguez Delgado, Miguel A.; García-Montelongo, Francisco J.; Cifuentes, Alejandro
2005-06-01

Digital.CSIC (Spain)

101

Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography

Gutiérrez Suárez, Ana; Río Andrade, José Carlos del; González-Vila, Francisco Javier; Martín Martínez, Francisco
1998-01-01

Digital.CSIC (Spain)

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ARIES a ready for use platform for engineering Spanish-processing tools

Gonzalez Cristobal, Jose Carlos; Goñi Menoyo, José Miguel; Nieto Serrano, Amalio Francisco

A lexical platform has been developed for the Spanish language both portable and efficient in terms of lexical coverage. Upon this platform a set of tools have been implemented including first versions of a spell checker, unication based parser and grammar and a stochastic morphosyntactic tagger.

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