Sample records for SORBOSA (sorbose)
from WorldWideScience.org

Sample records 1 - 5 shown.



1

Estudio de correlación entre cepas Escherichia coli sorbosa negativa y su capacidad de producir enterotoxinas/ Study of correlation between negative sorbose E. coli strains and their enterotoxin-producing capacity

Águila, Adalberto; Valdés-Dapena, Margarita; Ramírez, Margarita; Fernández, Carlos; Bravo, Laura
2006-04-01

Resumen en español Se estudiaron 50 cepas de Escherichia coli sorbosa negativas, aisladas de niños con diarreas líquidas de aparición espontánea. La identificación hasta especie se realizó por métodos convencionales, incluidos los marcadores fenotípicos sorbosa y sorbitol. Las técnicas de reacción en cadena de la polimerasa e hibridación de ADN, fueron realizadas para confirmar la capacidad de producción de enterotoxinas. Se obtuvo muy buena correlación entre las cepas de Esche (mas) richia coli sorbosa negativas y su capacidad de elaborar enterotoxinas termolábil y termoestable. El estudio demostró un alto porcentaje de estas cepas con fragmentos de genes codificadores, 94 y 44 % respectivamente, predominando la enterotoxina termoestable. Se obtuvieron los principales biotipos primarios de importancia taxonómica y epidemiológica. Se demostró que el método de la sorbosa posee cualidades que lo distinguen como marcador fenotípico, con 100 % de sensibilidad y 88 % de especificidad, revelándose como método de diagnóstico presuntivo práctico y de gran utilidad para los Laboratorios de Microbiología Clínica. Resumen en inglés Fifty negative sorbose Escherichia coli strains isolated from children who suffered spontaneously occurring fluid diarrheas were studied. Identification up to the level of species was performed by conventional methods including phenotypical markers known as sorbose and sorbitol. The PCR and DNA gene hybridization were applied to confirm the enterotoxin-producing capacity. A good correlation between negative sorbose Escherichia coli and their capacity of producing heat-lab (mas) ile and heat -stable enterotoxin was observed. The study showed a high percentage of these strains with encoding gene fragments, 94 and 44% respectively, being heat-stable enterotoxin the predominant. The main primary biotypes of taxonomic and epidemiological importance were obtained. It was demonstrated that the sorbose method has certain qualities that make it stand out as a phenotypical marker, with 100% sensitivity and 88% specificity, and as a practical presumptive diagnostic method that is very useful for Clinical Microbiology Laboratories.

Scientific Electronic Library Online (Spanish)

2

Genetics of L-sorbose transport and metabolism in Lactobacillus casei

Yebra, María Jesús; Veyrat, Ana; Santos, Mario A.; Pérez Martínez, Gaspar

Genes encoding L-sorbose metabolism of Lactobacillus casei ATCC 393 have been identified on a 6.8-kb chromosomal DNA fragment. Sequence analysis revealed seven complete genes and a partial open reading frame transcribed as two units, The deduced amino acid sequences; of the first transcriptional uni...

DRIVER (Spanish)

3

Genetics of L-sorbose transport and metabolism in Lactobacillus casei

Yebra, María Jesús; Veyrat, Ana; Santos, Mario A.; Pérez Martínez, Gaspar
2000-01-01

Digital.CSIC (Spain)

4

Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei.

Nissen, Lorenzo; Yebra, María Jesús; Sgobarti, Barbara; Pérez Martínez, Gaspar

DRIVER (Spanish)

5

Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei.

Nissen, Lorenzo; Yebra, María Jesús; Sgobarti, Barbara; Pérez Martínez, Gaspar
2005-04-01

Digital.CSIC (Spain)