Sample records for RIBONUCLEOSA (ribonuclease)
from WorldWideScience.org

Sample records 1 - 19 shown.



1

RIBONUCLEASAS: [subtitle]SU POTENCIAL TERAPÉUTICO EN INFECCIONES VIRALES/ Ribonucleases: [subtitle]Theurapetical potential on Viral Infections

ÚSUGA, XIOMARA; RUGELES, MARÍA TERESA
2006-06-01

Resumen en español En la actualidad existe un gran interés por identificar proteínas o péptidos antimicrobianos que puedan ser herramientas terapéuticas que eviten el establecimiento o permitan el control de diferentes infecciones. Las ribonucleasas (RNasas), pertenecientes a la superfamilia Ribonucleasa A, son enzimas que participan en varios procesos fisiológicos, que van desde el procesamiento alternativo del RNA hasta la angiogénesis. Estas enzimas son expresadas por diferentes te (mas) jidos y exhiben especificidades variables contra diferentes sustratos de RNA. El potencial terapéutico de las RNasas se ha sugerido en procesos oncogénicos; adicionalmente, se ha descrito que tienen actividad antiviral directa y el potencial de activar células del sistema inmune innato induciendo su maduración y la producción de citoquinas proinflamatorias. Nuestro grupo de investigación ha realizado estudios que señalan la capacidad de cuatro RNasas recombinantes: EDN, 4EDN, RNasa A y angiogenina de inhibir la replicación del virus de la inmunodeficiencia humana tipo 1 en linfocitos T de sangre periférica activados. En este artículo se revisará la clasificación de las ribonucleasas que constituyen la superfamilia RNasa A y se describirá, en forma detallada, lo que se conoce de la función biológica, acción antiviral y mecanismo de acción de las RNasas a las que se les ha reportado actividad antiviral. Resumen en inglés Currently, there is a great interest to identify proteins or antimicrobial peptides to be included in the therapeutic arsenal for preventing different infectious diseases. Ribonucleases (RNases) that belong to the Ribonuclease A superfamily participate in several physiologic processes, from alternative splicing of RNA to organogenesis. These enzymes are expressed by various tissues and exhibit variable specificities against different RNA substrates. The therapeutic potent (mas) ial of RNases has been suggested for oncogenic processes; in addition, direct antiviral activity and the potential to activate cells from the innate immune system, inducing their maturation and release of proinflammatory cytokines have been also associated with these enzymes. Our research team, have carried out studies that indicate the ability of four recombinant RNases: EDN, 4EDN, RNase A and angiogenin to inhibit HIV1 replication in activated peripheral blood T lymphocytes. In this article we review the classification of RNases that belong to the Ribonucleases A superfamily; we describe in detail what is known regarding the biologic function, inhibitory activity and mechanism of action of the RNases recognized by their antiviral activity.

Scientific Electronic Library Online (Spanish)

2

Polimorfismo G1385A del gen RNASEL y su asociación con el desarrollo de cáncer de próstata: Estudio preliminar/ G138A polymorphism of the RNASEL gene and its association with the development of prostate cancer: Preliminary study

Zabala, William; Delgado, Criserly; Pardo, Tatiana; Borjas, Lisbeth; Rojas-Atencio, Alicias; Reyes, Francia; Quintero, José Miguel
2009-09-01

Resumen en español El cáncer de Próstata (CAP), es una enfermedad compleja de origen multifactorial. Se caracteriza por patrones heterogéneos de crecimiento de tejido neoplásico, que varían ampliamente en su progresión, edad de aparición y respuesta al tratamiento. Se considera la segunda causa más común de muerte por malignidad en hombres y se estima que uno de cada cinco padece de CAP en el curso de su vida. La etiología genética de la transformación neoplásica de las célula (mas) s prostáticas normales aún es desconocida, sin embargo, investigaciones epidemiológicas han demostrado un fuerte componente genético en su desarrollo, y sugieren tanto un patrón de herencia mendeliana como la presencia de loci de susceptibilidad a lo largo del genoma humano. Se ha descrito una región cromosómica relacionada con el CAP denominada como HPC1, en el locus 1q24-25, donde se ubica el gen RNASEL, y las mutaciones en el mismo, se han asociado con la presencia del CAP en múltiples grupos familiares. EL gen RNASEL codifica para una ribonucleasa que degrada ARN viral y celular y que interviene en la apoptosis. Se ha reportado disminución de la actividad enzimática de hasta tres veces en portadores del polimorfismo G1385A de este gen, y la misma se ha asociado frecuentemente con el desarrollo del CAP. Mediante la utilización de una variante de la Reacción en Cadena de la Polimerasa (RCP), una amplificación alelo específica, se estudiaron 103 individuos masculinos con y sin CAP pertenecientes a la población de Maracaibo, Venezuela, evidenciándose ausencia de asociación. Resumen en inglés Prostate Cancer (CAP), is a complex disease with a multifactorial origin. It is characterized by heterogenous patterns of growth of neoplasic tissue, varying widely in its progression, age of beginning and therapy response. It is considered as the second most common cause of death by cancer in men and, it has been estimated, that one of five, suffers of CAP through the course of his life. The genetic etiology of neoplasic transformation of normal prostate cells is still n (mas) ot known; nevertheless, investigations in epidemiology have demonstrated a strong genetic component in its development, suggesting so much a pattern of mendelian inheritance as the presence of loci of susceptibility throughout the human genome. It has been described a cromosomic location related to the CAP in locus 1q24-25, denominated HPC1, where the gene RNASEL is located, and the seggregation of its alleles has been associated with the development of CAP in numerous familiar groups. The RNASEL gene codifies for a ribonuclease protein that degrades viral and cellular ARN and takes part in the apoptosis. A decrease of the enzymatic activity up to three times in carriers of the G1385A polymorphism of this gene has been reported, and the same has been associated frequently with the development of CAP. Using a variant of the Polymerase Chain Reaction, Allele specific amplification, this investigation had as objective to determine the association between variant G1385A and CAP, in a sample of 103 masculine individuals with and without CAP, pertaining to the population of Maracaibo, Venezuela, An association between these variants and CAP could not be demonstrated.

Scientific Electronic Library Online (Spanish)

4

Stylar ribonucleases in almond: correlation with and prediction of incompatibility genotypes

Bošković, Radovan I.; Tobutt, Kenneth R.; Batlle, I.; Duval, Henri; Martínez-Gómez, Pedro; Gradziel, Thomas M.
2003-01-01

Digital.CSIC (Spain)

5

Study of self-compatibility in almond progenies from self-fertilization by fluorescence microscopy and stylar ribonuclease assay

Ortega, Encarnación; Martínez-Gómez, Pedro; Dicenta, Federico; Bošković, Radovan I.; Tobutt, Kenneth R.
2002-01-01

Digital.CSIC (Spain)

6

Structural organization of a viral IRES depends on the integrity of the GNRA motif

Fernández-Miragall, Olga; Martínez-Salas, Encarnación
2003-01-01

Digital.CSIC (Spain)

7

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.

Araya, Alexandre; Zabaleta, Eduardo; Blanc, Valérie; Bégu, Dominique; Hernould, Michel; Mouras, Armand; Litvak, Simon
1998-04-01

Resumen en inglés RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to those from organisms where this process is absent. This biochemical process involves cytidine deamination. The cytoplasmic male sterility (CMS) phenotype generated by the inco (mas) mpatibility between the nuclear and the mitochondrial genomes is an important agronomical trait which prevents inbreeding and favors hybrid production. The hypothesis that RNA editing leads to functional proteins has been proposed. This hypothesis was tested by constructing transgenic plants expressing a mitochondrial protein translated from unedited mRNA. The transgenic "unedited" protein was addressed to the mitochondria leading to the appearance of mitochondrial dysfunction and generating the male sterile phenotype in transgenic tobacco plants. Male sterile plants were also obtained by expressing specifically a bacterial ribonuclease in the anthers. The economical benefits of artificially engineered male-sterile plants or carrying the (native) spontaneous CMS phenotype, implies the restoration to obtain fertile hybrids that will be used in agriculture. Restoration to fertility of transgenic plants was obtained either by crossing male-sterile plants carrying the "unedited" mRNA with plants carrying the same RNA, but in the antisense orientation or, in the case of plants expresing the ribonuclease, by crossing male-sterile plants with plants expressing an inhibitor specific of this enzyme

Scientific Electronic Library Online (Spanish)

8

Protein self-association in crowded protein solutions: a time-resolved fluorescence polarization study

Zorrilla, Silvia; Rivas, Germán; Acuña Fernandez, Alberto Ulises; Lillo, M. Pilar

The self-association equilibrium of a tracer protein, apomyoglobin (apoMb), in highly concentrated crowded solutions of ribonuclease A (RNase A) and human serum albumin (HSA), has been studied as a model system of protein interactions that occur in crowded macromolecular environments. The rotational...

DRIVER (Spanish)

9

Protein self-association in crowded protein solutions: a time-resolved fluorescence polarization study

Zorrilla, Silvia; Rivas, Germán; Acuña Fernandez, Alberto Ulises; Lillo, M. Pilar
2004-01-01

Digital.CSIC (Spain)

10

Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes

Almoguera, Concepción; Shibata, D.; Forrester, K.; Martin, J.; Amheim, N.; Perucho, M.
1988-01-01

Digital.CSIC (Spain)

12

Influence of Model Globular Proteins with Different Isoelectric Points on the Precipitation of Calcium Carbonate

Hernández-Hernández, A.; Rodríguez-Navarro, Alejandro; Gómez-Morales, J.; Jiménez-López, Concepción; Nys, Y.; García Ruiz, Juan Manuel
2008-04-11

Digital.CSIC (Spain)

13

Homozygous versus heterozygous self-compatible seedlings in an almond breeding programme

Dicenta, Federico; Ortega, Encarnación; Martínez-Gómez, Pedro; Bošković, Radovan I.; Tobutt, Kenneth R.
2002-01-01

Digital.CSIC (Spain)

14

High incidence of c-K-ras oncogenes in human colon cancer detected by the RNAse A mismatch cleavage method

Forrester, K.; Almoguera, Concepción; Jordano, Juan; Grizzle, W.E.; Perucho, M.
1987-01-01

Digital.CSIC (Spain)

15

Genotyping apricot cultivars for self-(in)compatibility by means of RNases associated with S alleles

Alburquerque, Nuria; Egea, José; Pérez-Tornero, Olaya; Burgos Ortiz, Lorenzo
2002-08-01

Digital.CSIC (Spain)

16
17

Comparison of homozygous and heterozygous self-compatible seedlings in an almond breeding programme

Dicenta, Federico; Ortega, Encarnación; Martínez-Gómez, Pedro; Bošković, Radovan I.; Tobutt, Kenneth R.
2002-03-01

Digital.CSIC (Spain)

18

Binding curves by continuous gradient flow-mix calorimetry

Martínez, Jose C.; López-Mayorga, Obdulio; Parody-Morreale, Antonio; Gavira Gallardo, J. A.
2005-10-01

Digital.CSIC (Spain)