Sample records for NEOMICINA (neomycin)
from WorldWideScience.org

Sample records 1 - 11 shown.



1

"Método de las 5 Placas" Para la Detección de Residuos de Antibacterianos en Leche./ "The Five Plate Method" for the Detection of Antibacterial Residues in Milk.

Gatica P., Cristina; Gesche R., Erika
2007-05-01

Resumen en español Los residuos de antibacterianos en leche traen consigo graves problemas, tanto para la salud pública como para la industria lechera, por lo que se hace necesario disponer de un método estandarizado, de bajo costo, de fácil implementación y capaz de detectar la mayoría de los antibacterianos de uso rutinario. Es por ello que el objetivo del presente trabajo fue la evaluación de la sensibilidad de una técnica microbiológica para la detección de estos residuos en le (mas) che, acorde a la evolución del uso de este tipo de medicamentos en Chile. Se propone el "Método de las 5 placas", el cual utiliza como cepas sensibles Bacillus stearothermophilus a pH 7,0; Bacillus subtilis a pH 6,0; 7,2 y 8,0 y Escherichia coli a pH 8,0. Se utilizaron los 18 antibacterianos de mayor oferta en Chile para vacas de lechería (ampicilina, cefoperazona, ceftiofur sódico, cloxacilina, penicilina G, pirlimicina, nafcilina, dihidroestreptomicina, gentamicina, neomicina, enrofloxacina, espiramicina, cloramfenicol, lincomicina, oxitetraciclina, tetraciclina, sulfadimidina y sulfadoxina) y se determinó la placa más sensible a cada uno de ellos. Cada antibacteriano analizado fue detectado en alguna de las 5 placas. Posteriormente, en la placa más sensible a cada antibacteriano y mediante la confección de curvas de calibración, se determinó la concentración mínima detectable para cada uno de éstos, resultando cada placa sensible a dos o más antibacterianos, demostrando así el método una adecuada sensibilidad a los antibacterianos analizados. Resumen en inglés Serious problem come out for public health as for the milk industry when antibiotics residues appear in milk products. It has become necessary to have a standarized method, of low cost, easy to perform and able to detect most of the antibiotics used now a days. The aim of this work was the evaluation of the sensibility of a novel microbiological technique able to detect these residues in milk, in order to go with the evolution of the use of these drugs in dairy cows in Ch (mas) ile. The technique proposed was named "The 5 Plates Method" and uses as sensible strains Bacillus stearothermophilus at pH 7.0, Bacillus subtilis at pH 6.0, 7.2 y 8.0 y Escherichia coli at pH 8.0. Eighteen antimicrobials where studied, those of greater offer in Chile for dairy cows (ampicillin, cefoperazone, ceftiofur, cloxacillin, penicillin G, pirlimycin, nafcillin, dihydrostreptomycin, gentamicin, neomycin, enrofloxacin, spiramycin, chloramphenicol, lincomycin, oxitetracycline, tetracycline, sulfadimidine and suldadoxin). The most sensitive plate was determined for each one of them. Each antimicrobials analysed with "The 5 Plates Method" was detected in one of the 5 plates. Later, on the plate determined as the most sensitive to each antimicrobial, calibration curves where made to point out the minimum detectable concentration for each one of them, resulting to be each plate sensitive to at least 2 or more antimicrobials demonstrating therefore that this method has an suitable sensitivity to these antimicrobial.

Scientific Electronic Library Online (Spanish)

2

Estudio epidemiológico de la dermatitis de contacto en personas mayores de 65 años/ Epidemiological study of the allergic contact dermatitis in people over 65 years old

Azúa Blanco, Mª Dolores; Piñol Monserrat, Juan; Navarro Lucía, Margarita
2009-09-01

Resumen en español La dermatitis de contacto (DAC) es una de las patologías ocupacionales o no más frecuentes, pudiendo constituir del 3% al 5% de las consultas de dermatología. El diagnóstico se realiza en base a la clínica presentada y a la realización de pruebas epicutáneas, pudiéndose demostrar con éstas la sustancia causante de esta enfermedad y valorar la prevalencia y la relevancia de las positividades de los alérgenos estudiados para hacer una buena prevención. Todos los (mas) pacientes fueron estudiados mediante la serie estándar GEIDC (Grupo Español de Investigación de Dermatitis de Contacto) (29 alérgenos) y con alérgenos homologados elaborados por Martitor España. La técnica de aplicación y lectura de los parches se realizó siguiendo las recomendaciones del GEIDC. Se estudiaron pacientes de 65 años y más, registrados durante 9 años en la sección de alergia HCU Lozano Blesa Área Sanitaria 3, con las siguientes variables: número de pacientes con pruebas (+), edad, sexo, patología presentada, localización de las lesiones, profesiones, positividades a cada alergia. Se estudiaron 1.559 pacientes y, de ellos, a 1.309 (el 83,96%) se les diagnosticó DAC, 551 varones (42,1%) y 758 mujeres (57,9%); de este grupo tan sólo 50 personas (el 3,8%) tenían más de 64 años, 17 varones y 33 mujeres. Revisando los datos de este estudio vemos que la alergia más prevalente es a medicamentos tópicos en 39 pacientes (37,14%), 26 mujeres y 13 varones, usados para el tratamiento de diversas afecciones dermatológicas. Pensamos que estos datos son interesantes y deben ser tenidos en cuenta al prescribir un tratamiento al paciente geriátrico, ya que podemos evitar el uso de determinadas sustancias con capacidad sensibilizante intensa (caínas, neomicina, etc.) sustituyéndolas por otros fármacos. Resumen en inglés Allergic Contact Dermatitis (CAD) is one of the most common pathologies of an occupational or non-occupational nature, making up from 3% to 5% of all dermatology consultations. Diagnosis is made from the presented clinical symptoms and by means of epicutaneous tests to identify the substance causing the condition, and to allow the prevalence and relevance of positive reactions to the studied allergens to be assessed in order to take suitable preventive action. All of the (mas) patients were studied with the standard Spanish Contact Dermatitis Research Group (GEIDC) skin tests and standard approved allergens produced by Martitor España guide lines. The stydy was made of patients aged 65 and over registered with the Hospital Clínico Universitario Lozano Blesa Area 3 Dermatological Allergy Unit for 9 years, with the following variables: number of patients with (+) test results, age, sex, pathology presented, location of lesions, occupation and positive reactions to each allergen. A total of 1.559 patients were studied. Of these, 1.309 (83.96%) were diagnosed with ACD, 551 males (42.10%) and 758 females (57.90%). Only 50 individuals from this group were over 64 years of age, 17 males and 33 females. On review of the data from the study, it was seen that the most prevalent allergy is to topical medications used for the treatment of a number of dermatological conditions in 39 patients (37.14%), 26 women and 13 males. We believe that these data are interesting and should be taken into account when prescribing treatments to geriatric patients. In this way, the use of specific substances with intense sensitizing capacities (cainas, neomycin, etc.) can be avoided and replaced with other drugs.

Scientific Electronic Library Online (Spanish)

3

Resistencia a las Fluoroquinolonas y Otros Antimicrobianos en Cepas de Salmonella spp. Aisladas en el Procesamiento de Pollo Entero./ Fluoroquinolone Resistance and Other Drugs in Salmonella spp. Strains Isolated from Whole Chicken Processing.

Briceño-Torres, Lilibeth; Narváez-Bravo, Claudia A; Rodas-González, Argenis; Wittum, Thomas E; Hoet, Armando E
2007-10-01

Resumen en español El objetivo de este estudio fue determinar patrones de resistencia y multirresistencia de cepas de Salmonella spp. aisladas de una planta procesadora de aves, hacia las quinolonas y fluoroquinolonas (ácido nalidíxico=Na, ciprofloxacina=Cf y enrofloxacina=Ex), así como a otros antimicrobianos: tetraciclinas (T), oxitetraciclina (O), neomicina (N), nitrofurantoina (Nf), trimetoprim (Tr) y cloranfenicol (C). Un total de 146 aislamientos de Salmonella spp. fueron obtenidos (mas) de diferentes fuentes: 34 cepas provenientes de mezclas de vísceras blancas (colón, ciegos) y vísceras rojas (hígado y bazo); 87 cepas aisladas de las canales en los procesos de desplume, eviscerado, enfriamiento y empacado; 8 cepas obtenidas de subproductos comestibles (patas, cuellos, hígados y mollejas) y 19 cepas de muestras de ambientes (agua, hielo y superficies de equipos). Se utilizaron técnicas microbiológicas convencionales, pruebas bioquímicas, serológicas y pruebas de susceptibilidad a los antibióticos por difusión en agar. Los resultados revelaron una alta resistencia para Na (73,3%; 107/146), Nf (60,2%; 88/146), T (56,2%; 82/146), O (54,8%; 80/146), Tr (54,1%; 79/146) y una menor resistencia a Ex (6.2%; 9/146), Cf (2,7%; 4/146), N (2,0%; 3/146) y C (2,5%; 4/146). Se encontró un porcentaje elevado de multirresistencia (65,0%; 95/146) y dentro de ellos, los más notorios fueron: NaNfTTr (42,1%), NaNfTr (26,3%) y NaNfT (10,5%). No se observó relación significativa (P>0,05) entre los patrones de resistencia y multirresistencia encontrados con el origen de las diferentes cepas de Salmonella. Estos resultados evidencian el surgimiento de cepas de Salmonellas resistentes a las quinolonas y la necesidad de programas de vigilancia de resistencia antimicrobiana. Resumen en inglés The aim of this study was to determine the resistance and multi-resistance patterns of strains of Salmonella spp. isolated in a poultry processing plant in Zulia State, to quinolones and fluoroquinolones (Nalidixic acid=Na, ciprofloxacin=Cf, and enrofloxacin=Ex), as well as other antimicrobial drugs: tetracycline (T), oxitetracycline (O), neomycin (N), nitrofurantoine (Nf), trimetropim (Tr) and chloranfenicol (C). A total of 146 Salmonella isolates were obtained from diff (mas) erent sources: 34 strains from pools of Intestines (duodenal and colon) and internal organs (liver and spleen); 87 strains of carcass samples collected in four different phases: carcasses after defeathering, evisceration, chilling, and final packed products; 8 strains from edible by-products (neck, liver, gizzard and legs) and 19 strains from environmental samples (water, ice, and equipment surfaces). The detection analyses were performed using conventional microbiological techniques, biochemical tests, serological and agar diffusion methods for antimicrobial susceptibility. The results showed a high resistance to Na (73.3%; 107/146), Nf (60.2%; 88/147), T (56.2%; 82/146), O (54.8%; 80/146), Tr (54.1%; 79/146) and low resistance to Ex (6.2%; 9/146), Cf (2.7%; 4/146), N (2.0%; 3/146) and C (2.7%; 4/146). There was observed a high percentage of multi-resistant strains (65.0%; 95/146) and within of them, the most common patterns were: NaNfTTr (42.1%), NaNfTr (26.3%) and NaNfT (10.5%). No significant relationship was observed (P>0.05) between resistance and multi-resistance patterns with the source of the Salmonella strains. These results are evidence of the emergence of resistant Salmonella strains to fluoroquinolones and the necessity of programs for antimicrobial resistance surveillance.

Scientific Electronic Library Online (Spanish)

5

Resistencia a gentamicina, amikacina y ciprofloxacina en cepas hospitalarias de Klebsiella pneumoniae subespecie pneumoniae productoras de ß-lactamasas de espectro extendido/ Resistance to gentamicin, amikacin and ciprofloxacin among nosocomial isolates of Klebsiella pneumoniae subspecie pneumoniae producing extended spectrum ß-lactamases

Díaz Q, Patricia; Bello T, Helia; Domínguez Y, Mariana; Trabal F, Natalia; Mella M, Sergio; Zemelman Z, Raúl; González R, Gerardo
2004-10-01

Resumen en inglés Background: Klebsiella pneumoniae is a pathogenic bacterium frequently isolated from nosocomial samples, specially the subspecie pneumoniae, with extensive antibiotic resistance profiles, including third generation cephalosporins, aminoglycosides and quinolones. This is specially true for those strains producing extended spectrum beta lactamases (ESBL). Aim: To investigate the susceptibility to gentamicin, amikacin and ciprofloxacin and the presence of some aminoglycoside (mas) modifying enzyme (AMEs) among nosocomial strains of K pneumoniae subspecie pneumoniae producing ESBL. Material and methods: The antibiotic resistant patterns and the level of resistance (minimal inhibitory concentration, MIC) of 100 strains, isolated from several hospitals of different Chilean cities, were determined. The presence of some aminoglycosides modifying enzyme (AMEs) was investigated by PCR. Results: Sixty five percent of strains were resistant to gentamicin, 47% were resistant to amikacin and 29% were resistant to ciprofloxacin. The most frequent AMEs genes detected were the aac(6')-Ib gene (6'-N-Acetyltransferase type Ib enzyme) in 69% of strains, conferring resistance to amikacin, kanamycin, tobramycin, and neomycin, and the gene aac(3)-IIa (3-Acetyltransferase type 3-IIa enzyme), in 36% of strains, conferring resistance to gentamicin. Conclusions: Among nosocomial strains of K pneumoniae subspecie pneumoniae isolated from Chilean hospitals, there is an association between the production of ESBL and the resistance to others antimicrobial agents, especially aminoglycosides. Nevertheless, 71% of isolates are susceptible to ciprofloxacin (Rev Méd Chile 2004; 132: 1173-8)

Scientific Electronic Library Online (Spanish)

6

Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile/ Molecular identification of aminoglycoside-modifying enzymes among strains of Enterococcus spp. isolated in hospitals of the VIII Region of Chile

Sepúlveda A, Marcela; Bello T, Helia; Domínguez Y, Mariana; Mella M, Sergio; Zemelman Z, Raúl; González R, Gerardo
2007-05-01

Resumen en inglés Background: Infectious diseases produced by Enterococcus spp, must be treated with a synergistic combination between a penicillin and an aminoglycoside. High level resistance to aminoglycosides is a serious therapeutic problem, since it predicts the loss of synergistic activity of this antimicrobial combination. Aim: To investigate the presence of genes encoding aminoglycoside-modifying enzymes (AMEs) among strains of Enterococcus spp with high level of resistance to amin (mas) oglycosides. Material and methods: The genes encoding some of the AMEs were investigated among 305 aminoglycoside-resistant strains of Enterococcus spp isolated in hospitals of the VIII region of Chile, by dot blot hybridization and Polymerase Chain Reaction (PCS). Results: High level resistance to some aminoglycosides was observed in 104 strains (34.1 %) and 93 of these harbored at ¡east one of the genes encoding the investigated AMEs. Three genes were detected: aac(6)Ie-aph(2")Ia (14.8%) encoding for the enzyme AAC(6)Ie-APH(2")Ia (resistance to all aminoglycosides, except streptomycin); aph(3)IIIa (26%), and ant(6)la (28.5%) encoding for the phosphorylating enzymes APH(3)Ilia (resistance to kanamycin, amikacin and neomycin), and ANT(6)-la (resistance only to streptomycin), respectively. None of the strains harbored the gene ant (4) which encode for the enzyme ANT (4). Conclusion: The low frequency of strains harbouring the bifunctional enzyme (

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7

Experimental evidence for the existence of non-exo-anomeric conformations in branched oligosaccharides: the neomycin-B case

Asensio, Juan Luis; Hidalgo, Ana; Cuesta, Igor; González, Carlos; Cañada Vicinay, Francisco Javier; Vicent, Cristina; Chiara, José Luis; Cuevas, Gabriel; Jiménez-Barbero, Jesús
2002-01-01

Digital.CSIC (Spain)

8

Experimental Evidence for the Existence of Non-exo-Anomeric Conformations in Branched Oligosaccharides: NMR Analysis of the Structure and Dynamics of Aminoglycosides of the Neomycin Family

Asensio, Juan Luis; Hidalgo, Ana; Cuesta, Igor; González, Carlos; Cañada Vicinay, Francisco Javier; Vicent, Cristina; Chiara, José Luis; Cuevas, Gabriel; Jiménez-Barbero, Jesús
2002-11-01

Digital.CSIC (Spain)

9

Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector

Fuerstenberg, S.; Beug, H.; Introna, M.; Khazaie, K.; Muñoz, A.; Ness, S.; Nordström, Kristina; Sap, J.; Stanley, I.; Zenke, M.; Vennström, Björn
1990-12-01

Digital.CSIC (Spain)

11

A Simple Structural-Based Approach to Prevent Aminoglycoside Inactivation by Bacterial Defense Proteins. Conformational Restriction Provides Effective Protection against Neomycin-B Nucleotidylation by ANT4

Asensio, Juan Luis; Hidalgo, Ana; Bastida, Ágatha; Torrado, Mario; Corzana, Francisco; Chiara, José Luis; García-Junceda, Eduardo; Cañada Vicinay, Francisco Javier; Jiménez-Barbero, Jesús
2005-01-01

Digital.CSIC (Spain)