Sample records for SONDAS IONICAS (ion probes)
from WorldWideScience.org

Sample records 1 - 16 shown.



1

The hydration of Cu2+: Can the Jahn-Teller effect be detected in liquid solution?

Chaboy, J.; Muñoz-Páez, Adela; Merkling, Patrick J.; Sánchez Marcos, Enrique
2006-02-01

Digital.CSIC (Spain)

2

Redistribution of alloying elements during tempering of a nanocrystalline steel

Caballero, F. G.; Miller, M. K.; García-Mateo, C.; Capdevila, C.; Babu, S. S.
2008-01-01

Digital.CSIC (Spain)

3

Papel de la vacuola parasitófora de macrófagos de ratón infectados por Leishmania amazonensis en la adquisición de moléculas/ Role of the parasitophorous vacuole of murine macrophages infected with Leishmania amazonensis in molecule acquisition

Cortázar, Tania M; Hernández, Joselín; Echeverry, María Clara; Camacho, Marcela
2006-10-01

Resumen en español Introducción. Leishmania son parásitos intracelulares de macrófagos, confinados en compartimentos denominados vacuolas parasitóforas. La permeabilidad de este compartimento depende de su interacción con el tráfico vesicular y transportadores presentes en su membrana. Objetivo. En este trabajo se estudió la permeabilidad de la membrana de la vacuola parasitófora en la línea celular J774.A1 infectada con Leishmania amazonensis, in situ y en compartimentos aislados. (mas) Materiales y métodos. El aislamiento de vacuolas parasitóforas se hizo por gradiente de densidad. La permeabilidad de la membrana de estas se valoró por distribución de sondas fluorescentes y electrofisiología. Para establecer indirectamente el transporte de protones se usó naranja de acridina. La presencia de transportadores ABC sensibles a probenecid se estableció con amarillo lucifer y calceína. Por primera vez con la técnica de patch-clamp se registraron corrientes en la membrana de este compartimento aislado. Resultados. La vacuola parasitófora colorea de rojo con naranja de acridina indicando un pH ácido. Concentra amarillo lucifer a través de un transportador sensible a probenecid, pero excluye la sonda calceína. Vacuolas aisladas se marcan de rojo con naranja de acridina y concentran amarillo lucifer a través de un transportador sensible a probenecid. Estas vacuolas excluyeron calceína y presentaron en su membrana una corriente iónica que se activa a diferencias de potencial cercanas a 60 mV, con una conductancia de 46 ± 3 pS. Conclusiones. Se pueden aislar vacuolas parasitóforas con propiedades de permeabilidad que preservan mecanismos de transporte similares a los encontrados in situ. Se registra por primera vez la presencia de una corriente iónica poco selectiva en la membrana de este compartimiento. Resumen en inglés Introduction. Leishmania are intracellular parasites of macrophages, confined into compartments known as parasitophorous vacuoles. The permeability of this compartment depends on its interaction with the endocytic pathway and transport proteins present on its membrane. Objective. The membrane permeability of the parasitophorous vacuole was studied in J774.A1- macrophage like cells infected with Leishmania amazonensis, in situ and on isolated compartments. Materials and me (mas) thods. The parasitophorous vacuoles were isolated by density gradients. Fluorescent probe distribution and electrophysiological recordings were used to determine parasitophorous vacuole membrane permeability. Proton transport was evaluated indirectly by acridine orange staining. Probenecid sensitive ABC transporters were detected using the fluorescent probes lucifer yellow and calcein. For the first time ion currents were recorded on the membrane of isolated parasitophorous vacuoles using the patch clamp technique. Results. The parasitophorous vacuole stains red with acridine orange indicating an acidic compartment. It concentrates lucifer yellow by means of a probenecid sensitive transporter but excludes calcein. Isolated vacuoles stained red with acridine orange and concentrated lucifer yellow by means of a probenecid sensitive transporter. These vacuoles excluded calcein and showed an ion current in their membrane which is activated at potentials close to 60 mV with a mean conductance of 46 ± 3 pS. Conclusions. Isolated parasitophorous vacuoles with permeability properties preserving transport mechanisms similar to those found in situ can be purified. A poorly selective ion current on the parasitophorous vacuole membrane is reported for the first time.

Scientific Electronic Library Online (Spanish)

5

Molecular determinants of stereoselective bupivacaine block of hKv1.5 channels

Franqueza, Laura; Longobardo, Mónica; Vicente, Javier; Delpón, Eva; Tamkun, Michael M; Tamargo, Juan; Snyders, Dirk J.; Valenzuela, Carmen
1997-11-01

Digital.CSIC (Spain)

6

Microscopic and macroscopic dielectric description of mixed oxide thin films

Ferrer, F. J.; Yubero Valencia, Francisco; Mejías, J. A.; García-López, F. J.; González-Elipe, Agustín R.
2007-10-30

Digital.CSIC (Spain)

7

Low-Pressure DC Air Plasmas. Investigation of Neutral and Ion Chemistry

Castillo, Marco; Méndez, Isabel; Islyaikin, A. M.; Herrero, Víctor J.; Tanarro, Isabel
2005-06-28

Digital.CSIC (Spain)

9

Imaging transient species in the femtosecond A-band photodissociation of CH3I

Durá, J.; Nalda, R. de; Amaral, G. A.; Bañares, Luis
2009-10-07

Digital.CSIC (Spain)

10

ISFET Based Microsensors for Environmental Monitoring

Jiménez-Jorquera, Cecilia; Orozco, Jahir; Baldi Coll, Antonio
2010-01-01

Digital.CSIC (Spain)

11

Fluorescent taxoids as probes of the microtubule cytoskeleton

Evangelio, Juan A.; Abal, Miguel; Barasoain, Isabel; Souto, André A.; Lillo, M. Pilar; Acuña Fernandez, Alberto Ulises; Amat-Guerri, Francisco; Andreu, José Manuel
1998-01-01

Digital.CSIC (Spain)

12

Environmentally Sensitive Paramagnetic and Diamagnetic Contrast Agents for Nuclear Magnetic Resonance Imaging and Spectroscopy

Pacheco, Jesús; Calle, Daniel; Lizarbe, Blanca; Negri, Viviana; Ubide, Carmen; Fayos, Rosa; López Larrubia, Pilar; Ballesteros García, Paloma; Cerdán García-Esteller, Sebastián
2011-01-01

Digital.CSIC (Spain)

15

Borderline microporous-ultramicroporous palladium(II) coordination polymer networks. Effect of pore functionalisation on gas adsorption properties

Navarro, Jorge A. R.; Barea, Elisa; Salas, Juan M.; Masciocchi, Norberto; Galli, Simona; Sironi, Angelo; Ovín Ania, M.ª Concepción; Parra Soto, José Bernardo
2007-02-23

Digital.CSIC (Spain)