Sample records for EGTA (egta)
from WorldWideScience.org

Sample records 1 - 4 shown.



1

Disrupción de las uniones mediadas por caderinas. Su rol en la apoptosis de las células granulosas del ovario porcino/ Disruption of junctions mediated by cadherins. Role in apoptosis of porcine ovarian granulosa cells

Lombardo, DM; Medina, JM; Revilla, M; Carou, CM; Fiorito, CD
2010-06-01

Resumen en español La fisiología normal de los tejidos reproductivos dependería de un apropiado contacto célula-célula mediado por caderinas, moléculas Ca++ dependientes. El proceso de la atresia dado por apoptosis de las células de granulosa (CG), podría estar relacionado con la pérdida de los contactos celulares. El objetivo del trabajo fue establecer la relación entre la disrupción de estas uniones y la apoptosis de las CG. Considerando la importancia de las caderinas (E-CAM y (mas) N-CAM) en el desarrollo y en la atresia folicular, que la mayoría de los folículos empiezan a degenerar en el estadio antral temprano y considerando la atresia como un proceso estadio-dependiente se planteó identificar y localizar la expresión de estas moléculas en el ovario de cerdo adulto, tratando de discriminar y relacionarla con los distintos estadios de la folículo génesis. En los folículos antrales, aquellas células que presentan una aparente pérdida de integridad (falta de cohesión con células vecinas o desagregadas de la capa de células antrales), no se identificó marcación alguna para los anticuerpos en cuestión. Con la finalidad de correlacionar el mismo proceso in vitro, se realizaron cultivos primarios de CG de ovarios porcinos obtenidos por punción en folículos antrales. Los cultivos fueron expuestos por 6, 12 y 24 hs a diferentes concentraciones del inductor de apoptosis, concentraciones de 100 y 9 nM de EGTA (quelante exclusivo de Ca++). Se determinó índice relativo de apoptosis (IRA) mediante la cuantificación de las células teñidas con DAPI, TUNEL e inmunocitoquímica (ICQ) con anticuerpos anti Bax. Del mismo modo se determinó el porcentaje de viabilidad por exclusión con Azul Tripán. Se observaron manifestaciones evidentes de apoptosis en los diferentes tratamientos con EGTA, sin embargo las diferencias entre el porcentaje de viabilidad y los IRA por DAPI no son significativas respecto del control. Los resultados observados fueron validados cuali y cuantitativamente por TUNEL e ICQ anti Bax. Resumen en inglés The normal physiology and the dynamics of the reproductive tissue depends in great measure of an appropriate contact cell to cell, which is mediated by proteins of the cadherins family, molecules Ca++ dependent such as the E and N Cadherin. In these circumstances, the atresia process for apoptosis of the granulosa cells (CG), it could be related with the loss of this cellular contacts. In this work, the objective was to establish the relationship among the loss of the con (mas) tacts Ca++ dependent with the integrity and the apoptosis of the CG. Considering the importance of cadherin (E-CAM and N-CAM) in the development and follicular atresia, which most of the follicles begin to degenerate in the early antral stage and considering the atresia as a stage-dependent process was raised identify and localize the expression of these molecules in the ovary of adult pig, trying to discriminate and relate the different stages of follicle genesis. En antral follicles, those cells with an apparent loss of integrity (lack of cohesion with neighboring cells or disaggregated antral cell layer), did not identify any other label for antibodies in question. In order to correlate the same process in vitro, the study model was a cellular primary culture of porcine CG obtained by aspiration of antrals follicles, which were treated with EGTA, to obtain the disruptión of the homophilic juntions. The treatments were made with EGTA 9 nM and 100 nM and without EGTA, both treatment at different times of induction 6, 12 and 24 hours. In each case, the apoptosis index (AI) was determined by DAPI and TUNEL techniques. Also, was carried out a quantitative evaluation by analysis of images of the expression of Bax by inmuno cytochemical. To different concentration of the inductor, the treatments with serum demonstrated a bigger AI to 9 nM (1,27; 2,58 and 1,38 to 6, 12 and 24 hs respectively). The activation was significant in the treatments of 12 hs with serum (p=0,05) respect to the control. To concentrations of 100 nM the AI didn't demonstrate significant differences of activation. The quantitative determination of the expression of Bax and for ICQ validates the previous results.

Scientific Electronic Library Online (Spanish)

2

Efecto in vitro del suero de la trucha Arco Iris (Oncorhynchus mykiss) sobre metacercarias del género Tylodelphys (Trematoda, Diplostomatidae)./ The in vitro effect of rainbow trout (Oncorhynchus mykiss) serum in Tylodelphys sp. (Trematoda, Diplostomatidae) metacercariae.

Olabuenaga, Susana
2000-01-01

Resumen en inglés Here, it has been described that normal fresh sera from rainbow trout (Oncorhynchus mykiss) were able to lyse Tylodelphys sp. metacercariae isolated from Galaxias maculatus (Jenyns, 1842) (Teleostei, Galaxiidae) brain. This effect was time dependent, and progressively increased since 1 h up to 18 h incubation. There were oscillations in the ability of different sera samples to lyse these parasites. Similar values were achieved with other salmonid sera, and also with norma (mas) l human sera pool. The lytic activity was abolished by heat-inactivated sera, by zymosan treatment and by incubation in the presence of EDTA. However, the mean values obtained in the presence of EGTA-Mg++ did not significantly differ from those found with sera as a source of complement. The results of this study suggested the role of complement alternative pathway on Tylodephys sp. in vitro lysis, under our experimental conditions

Scientific Electronic Library Online (Spanish)

3

The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein

Li, Hongbing; Sánchez-Torres, Juan; Carpio, Alan del; Salas, Valentina; Villalobo, Antonio
2004-07-01

Digital.CSIC (Spain)

4

Identification of possible targets of the Aspergillus fumigatus CRZ1 homologue, CrzA

Soriani, Frederico M.; Malavazi, Iran; Savoldi, Marcela; Espeso, Eduardo; Dinamarco, Taísa M.; Bernardes, Luciano AS; Ferreira, Márcia ES; Goldman, Maria Helena S.; Goldman, Gustavo H.
2010-01-15

Digital.CSIC (Spain)