Sample records for DROSOFILA (drosophila)
from WorldWideScience.org

Sample records 1 - 20 shown. Select sample records:



1

Drosophila as a model system for genetic and genomic research

Molnar, Cristina; Cruz, Cristina; Terriente, Ana; de Celis, Jose F.

The sequencing of the Drosophila genome allowed the identification of most codingsequences, highlighting the necessity for a functional assignation of the identified genes.The information extracted from the sequence directly classified a considerable fractionof genes into known molecular categori...

DRIVER (Spanish)

2

Drosophila as a model system for genetic and genomic research

Molnar, Cristina; Cruz, Cristina; Terriente, Ana; de Celis, Jose F.
2006-01-01

Digital.CSIC (Spain)

4

Drosophila GENE experiment in the Spanish Soyuz mission to the ISS: II. effects of the containment constraints

Herranz, R.; Lavan, D.A.; Medina, F.J.; Loon, J.J.W.A. van; Marco, R.

In the GENE experiment performed during an 11-day Soyuz Mission to the International Space Station (ISS), we intended to determine if microgravity aff ects Drosophila metamorphosis processes. Control experiments were performed including a 1g ground control parallel to the ISS flight samples and a Ra...

DRIVER (Spanish)

5

Drosophila Behaviour & Gene expression in altered gravity conditions: Comparison between Space and ground facilities

Herranz, Raul; Lavan, D. A.; Dijkstra, Camelia; Larkin, Oliver; Davey, Michael; Medina, F. Javier; van Loon, Jack; Marco, Roberto; Schiller, Peter
2008-06-27

Digital.CSIC (Spain)

6

Global patterns of sequence evolution in Drosophila

Gallach, Miguel; Arnau, Vicente; Marín, Ignacio

This article is available from: http://www.biomedcentral.com/1471-2164/8/408 | [Background] Sequencing of the genomes of several Drosophila allows for the first precise analysesof how global sequence patterns change among multiple, closely related animal species. A basicquestion is whether there a...

DRIVER (Spanish)

7

Global patterns of sequence evolution in Drosophila

Gallach, Miguel; Arnau, Vicente; Marín, Ignacio
2007-11-09

Digital.CSIC (Spain)

8

Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression

McCaffrey, Ruth; St Johnston, Daniel; González-Reyes, Acaimo

PMCID: PMC1667076 | mus301 was identified independently in two genetic screens, one for mutants hypersensitive to chemical mutagens and another for maternal mutants with eggshell defects. mus301 is required for the proper specification of the oocyte and for progression through meiosis in the Drosoph...

DRIVER (Spanish)

10

Stem cells, niches and cadherins: a view from Drosophila

González-Reyes, Acaimo

Stem cells are essential for the correct development and homeostasis of adult organisms, as well as having obvious potential therapeutic importance. Analysis of the biology of stem cells and their regulatory microenvironment in adult organs has, however, been hindered by the rarity of these cells in...

DRIVER (Spanish)

12

Función del factor LIM-HD Tailup en el notum de "Drosophila"

Navascués, Joaquín de

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura 20-07-2007 | [BACKGROUND] In Drosophila, the notum territory of the wing disc is specifiedduring the second larval instar. At this stage, notum formation dep...

DRIVER (Spanish)

13

Función del factor LIM-HD Tailup en el notum de "Drosophila"

Campuzano, Sonsoles; Navascués, Joaquín de
2007-01-01

Digital.CSIC (Spain)

14

The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis

Abdu, Uri; González-Reyes, Acaimo; Ghabrial, Amin; Schüpbach, Trudi

Sequence data from this article have been deposited with the Gen-Bank data libraries under accession no. AY257540. | In Drosophila, mutations in double-strand DNA break (DSB) repair enzymes, such as spn-B, activate a meiotic checkpoint leading to dorsal-ventral patterning defects in the egg and an a...

DRIVER (Spanish)

15

The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis

Abdu, Uri; González-Reyes, Acaimo; Ghabrial, Amin; Schüpbach, Trudi
2003-09-01

Digital.CSIC (Spain)

17

The Drosophila nuclear factor DREF positively regulates the expression of the mitochondrial transcription termination factor DmTTF

Fernández-Moreno, Miguel Á; Bruni, Francesco; Adán, Cristina; Hernández Sierra, Rosana; Loguercio Polosa, Paola; Cantatore, Palmiro; Garesse, Rafael; Roberti, Martina
2009-03-01

Digital.CSIC (Spain)

18

DeadEasy Caspase: Automatic Counting of Apoptotic Cells in Drosophila

Drosophila; Forero, Manuel G.; Pennack, Jenny A.; Learte, Anabel R.; Hidalgo, Alicia
2009-05-05

Digital.CSIC (Spain)

19

A new role for hth in the early pre-blastodermic divisions in drosophila.

Salvany, Lara; Aldaz, Silvia; Corsetti, Elise; Azpiazu, Natalia
2009-09-01

Digital.CSIC (Spain)

20

Role of oocyte position in establishment of anterior-posterior polarity in Drosophila

González-Reyes, Acaimo; St Johnston, Daniel

Final full-text version of the paper available at: http://dx.doi.org/10.1126/science.7939717 | The polarized microtubule cytoskeleton of the Drosophila oocyte directs the localization of the maternal determinants which establish the anterior-posterior (AP) axis of the embryo. Because the formation o...

DRIVER (Spanish)

22

Effect of the Gene doublesex of Anastrepha on the Somatic Sexual Development of Drosophila .

Alvarez, Mercedes; Ruiz Lorenzo, María Fernanda; Sánchez, L.

8 pages, 4 figures and 2 tables. | [Background]The gene doublesex (dsx) is at the bottom of the sex determination genetic cascade and is transcribed in both sexes, but gives rise to two different proteins, DsxF and DsxM, which impose female and male sexual development respectively via the sex-speci...

DRIVER (Spanish)

23

Effect of the Gene doublesex of Anastrepha on the Somatic Sexual Development of Drosophila.

Alvarez, Mercedes; Ruiz Lorenzo, María Fernanda; Sánchez, L.
2009-04-02

Digital.CSIC (Spain)

24

Characterization of dSnoN and its relationship to Decapentaplegic signaling in Drosophila

Barrio, Rosa; López-Varea, Ana; Casado, Mar; de Celis, Jose F.

Article available at http://dx.doi.org/10.1016/j.ydbio.2007.02.039 | Vertebrate members of the ski/snoN family of proto-oncogenes antagonize TGFβ and BMP signaling in a variety of experimental situations. This activity of Ski/SnoN proteins is related to their ability to interact with Smads, the prot...

DRIVER (Spanish)

25

Characterization of dSnoN and its relationship to Decapentaplegic signaling in Drosophila

Barrio, Rosa; López-Varea, Ana; Casado, Mar; de Celis, Jose F.
2007-03-03

Digital.CSIC (Spain)

26

La conducta de larvas de Drosophila (Diptera; Drosophilidae): su etología, desarrollo, genética y evolución/ The behavior of Drosophila larvae: their ethology, development, genetics and evolution

GODOY-HERRERA, RAÚL
2001-03-01

Resumen en español Este trabajo, en honor al Profesor Doctor Danko Brncic Juricic (Q.E.P.D.), es una revisión de nuestras contribuciones sobre la etología, desarrollo, genética y evolución de patrones de conducta de larvas de Drosophila. Se discute el desarrollo de conductas larvales de forrajeo y sus bases hereditarias. También se discuten estrategias de investigación dirigidas a entender las relaciones entre genotipo y conducta durante el desarrollo de los organismos. Se relacionan (mas) patrones de desarrollo de conductas larvales con la filogenia de las especies del grupo mesophragmatica de Drosophila. Finalmente, se distingue entre evolución de elementos de conducta simple y evolución de conductas complejas Resumen en inglés This is a review about our contributions in ethology, development, genetics, and evolution of larval behavioral patterns of Drosophila in honor of the late Professor Doctor Danko Brncic Juricic. The developmental behavioral genetics of larval foraging and pupation of Drosophila are discussed. It is also emphasized the importance of research strategies lead to understand properly the relationships between genotype and behavior during development of the organisms. Finally, (mas) a comparison between phylogenetic relationships of six Drosophila species of the mesophragmatica group and their developmental patterns of larval behaviors is provided

Scientific Electronic Library Online (Spanish)

27

DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Sotillos, Sol; Díaz-Meco, María T.; Caminero, Eva; Moscat, Jorge; Campuzano, Sonsoles

Both in Drosophila and vertebrate epithelial cells, the establishment of apicobasal polarity requires the apically localized, membrane-associated Par-3–Par-6–aPKC protein complex. In Drosophila, this complex colocalizes with the Crumbs–Stardust (Sdt)–Pals1-associated TJ protein (Patj) complex. Genet...

DRIVER (Spanish)

28

DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila

Sotillos, Sol; Díaz-Meco, María T.; Caminero, Eva; Moscat, Jorge; Campuzano, Sonsoles
2004-08-09

Digital.CSIC (Spain)

29

The G protein-coupled receptor regulatory kinase GPRK2 participates in Hedgehog signaling in Drosophila

Molnar, Cristina; Holguin, Elena; Mayor Jr., Federico; Ruiz-Gómez, Ana; de Celis, Jose F.

Article available at http.//dx.doi.org/10.1073/pnas.0702374104 | Signaling by Smoothened (Smo) plays fundamental roles during animal development and is deregulated in a variety of human cancers. Smo is a transmembrane protein with a heptahelical topology characteristic of G protein-coupled receptors...

DRIVER (Spanish)

30

The G protein-coupled receptor regulatory kinase GPRK2 participates in Hedgehog signaling in Drosophila

Molnar, Cristina; Holguín, Helena; Mayor Menéndez, Federico; Ruiz-Gómez, Ana; de Celis, Jose F.
2007-05-01

Digital.CSIC (Spain)

31

Tufted Is a gain-of-function allele that promotes ectopic expression of the proneural gene amos in Drosophila

Villa-Cuesta, Eugenia; de Navascués, Joaquín; Ruiz-Gómeza, Mar; Diez del Corral, Ruth; Domínguez, María

The Tufted1 (Tft1) dominant mutation promotes the generation of ectopic bristles (macrochaetae) in the dorsal mesothorax of Drosophila. Here we show that Tft1 corresponds to a gain-of-function allele of the proneural gene amos that is associated with a chromosomal aberration at 36F–37A. This causes ...

DRIVER (Spanish)

32

Tufted Is a gain-of-function allele that promotes ectopic expression of the proneural gene amos in Drosophila

Villa-Cuesta, Eugenia; Navascués, Joaquín de; Ruiz-Gómez, Mar; Diez del Corral, Ruth; Domínguez, María; de Celis, Jose F.; Modolell, Juan
2003-01-01

Digital.CSIC (Spain)

33

Genetic dissection of a stem cell niche: the case of the Drosophila ovary

Bolívar, Jorge; Pearson, John; López-Onieva, Lourdes; González-Reyes, Acaimo

Final full-text version of the paper available at: http://dx.doi.org/10.1002/dvdy.20967.-- The Supplementary Material referred to in this article can be viewed at www.interscience.wiley.com/jpages/1058-8388/suppmat | In this work, we demonstrate a powerful new tool for the manipulation of the stroma...

DRIVER (Spanish)

34

Genetic dissection of a stem cell niche: the case of the Drosophila ovary

Bolívar, Jorge; Pearson, John; López-Onieva, Lourdes; González-Reyes, Acaimo
2006-11-01

Digital.CSIC (Spain)

35

Función del gen lines y la fmailia odd-skipped en la integración de múltiples vías de señalización durante el desarrollo del ala de drosophila

Benítez de Gracia, Elvira

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura 29-02-2008 | This thesis aim to investigate the lines function during the imaginal wing discdevelopment. It was found that lines mutant cells induce an aberr...

DRIVER (Spanish)

37

A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila

de Navas, Luis; Foronda, David; Suzanne, Magali; Sánchez-Herrero, Ernesto

Article available at http://dx.doi.org/10.1016/j.mod.2006.07.010 | The functional replacement of one gene product by another one is a powerful method to study specificity in development and evolution. In Drosophila, the Gal4/UAS method has been used to analyze in vivo such functional substitutions. ...

DRIVER (Spanish)

39

A novel mutant phenotype implicates dicephalic in cyst formation in the Drosophila ovary

McCaffrey, Ruth; St Johnston, Daniel; González-Reyes, Acaimo

Final full-text version available at: http://dx.doi.org/10.1002/dvdy.20620.-- Published online 28 October 2005 in Wiley InterScience (www.interscience.wiley.com). | The establishment of polarity in Drosophila requires the correct specification of the oocyte in early stages of oogenesis, its position...

DRIVER (Spanish)

40

A novel mutant phenotype implicates dicephalic in cyst formation in the Drosophila ovary

McCaffrey, Ruth; St Johnston, Daniel; González-Reyes, Acaimo
2006-04-01

Digital.CSIC (Spain)

42

Análisis morfométrico en especies de Drosophila (Diptera: Drosophilidae) del grupo repleta de ecosistemas semiáridos colombianos/ Morphometric analysis in species of Drosophila (Diptera: Drosophilidae) of group repleta of semiarid Colombian ecosystems

E. DE POLANCO, MARÍA MAGDALENA; PRIETO S, RODRIGO; GALINDO, LUISA F; LOZANO B, LUIS A; ORDÓÑEZ V, MARINA
2008-06-01

Resumen en español El presente trabajo evaluó los aedeagos de los machos de las especies Drosophila martensis, D. starmeri, D. uniseta y D. aldrichi recolectadas en las poblaciones colombianas de Camarones cerca de Riohacha, Santa Marta (Costa Norte) y Rosalía en el Desierto de la Tatacoa (Huila). Este carácter es uno de los que ha presentado más rápida evolución, favorecida por selección natural en los procesos de formación de nuevas especies, especialmente en insectos, por lo tant (mas) o es considerado un adecuado indicador para la determinación del avance de los mencionados procesos. El análisis morfométrico con descriptores de Fourier elípticos y el MANOVA-CVA realizado mostró que entre las poblaciones de D. martensis (Pl wilk = 0,1816) al igual que en D. uniseta (Pl wilk = 0,0507) no existieron diferencias estadísticas en la forma del aedeago, contrario a lo obtenido para D. starmeri (Pl wilk = 0,0004). Esta misma prueba determinó que en las tres especies del conglomerado martensis la genitalia de los machos mostró diferencias significativas (Pl wilk = 4,23E-163). Se evidencia que las tres especies del conglomerado martensis mantienen su identidad taxonómica en el análisis. Así mismo, al comparar cada una de las especies del conglomerado con D. aldrichi, otra especie del grupo repleta recolectada en Tatacoa y perteneciente al complejo mulleri, se encuentra que los datos de D. martensis aparecieron sobrepuestos con los de D. aldrichi, lo cual estaría mostrando que existe una relación filogenética más cercana entre estas dos especies que entre D. martensis y las otras de su mismo conglomerado. Resumen en inglés The present work evaluated the aedeagus of males of the species D. martensis, D. starmeri, D. uniseta and D. aldrichi collected in the Colombian localities of Camarones near Riohacha, Santa Marta (North Coast) and Rosalía in the Tatacoa Desert (Huila Department). This character is one of those that has shown the most rapid evolution, favored by natural selection in the processes of new species formation, particularly in insects, and therefore it is considered an adequate (mas) indicator to determine the advancement of the aforementioned processes. Morphometric analysis with Fourier elliptical and MANOVA-CVA descriptors showed that among populations of D. martensis (Pl wilk= 0.1816), as well as D. uniseta (Pl wilk = 0.0507), there were no statistical differences in the form of the aedeagus, as opposed to that obtained for D. starmeri (Pl wilk = 0.0004). This same test determined that in the three species of the martensis cluster, the genitalia of the males demonstrated significant differences (Pl wilk = 4.23E-163). These results are evidence that each species in the martensis cluster maintains its taxonomic identity in the analysis. Likewise, when comparing each species of the cluster with D. aldrichi, another species of the replete group collected in Tatacoa and belonging to the mulleri cluster, it was found that the data of D. martensis appeared to be superimposed over those of D. aldrichi, which would show the existence of a closer phylogenetic relation among these two species, than between D. martensis and others of the same cluster.

Scientific Electronic Library Online (Spanish)

43

Comparative analysis of Drosophila melanogaster and Caenorhabditis elegans gene expression experiments in the European Soyuz flights to the International Space Station

Leandro, L. J.; Szewczyk, N. J.; Benguria, Alberto; Herranz Barranco, Raúl; Laván, Alfonso; Medina, Francisco J.

The European Soyuz missions have been one of the main routes for conducting scientific experiments onboard the International Space Station, which is currently in the construction phase. A relatively large number of life and physical sciences experiments as well as technology demonstrations have been...

DRIVER (Spanish)

44

Comparative analysis of Drosophila melanogaster and Caenorhabditis elegans gene expression experiments in the European Soyuz flights to the International Space Station

Leandro, L. J.; Szewczyk, N. J.; Benguria, Alberto; Herranz, Raúl; Laván, Alfonso; Medina, Francisco J.; Gasset, Gilbert; Loon, J. van; Conley, C. A.; Marco, Roberto
2007-04-01

Digital.CSIC (Spain)

45

A Conserved Motif in Goosecoid Mediates Groucho-Dependent Repression in Drosophila Embryos

Jiménez, Gerardo; Verrijzer, C. Peter; Ish-Horowicz, David
1999-03-01

Digital.CSIC (Spain)

46

Aspectos del polimorfismo genético en Drosophila pseudoobscura, D. willistoni y D. nebulosa/ Aspects of genetic polymorphism in Drosophila pseudoobscura, D. willistoni and D. nebulosa

Salceda Sacanelles, Víctor Manuel
2008-06-01

Resumen en español En México el polimorfismo cromosómico en poblaciones naturales de Drosophila se estudió en D. pseudoobscura, D. willistoni y D. nebulosa. En la primera, más ampliamente analizada, se reconocen 27 inversiones correspondientes al 67.5% de la variabilidad para esta característica, identificando cuatro razas. Diferentes recolectas detectaron nuevas inversiones que permitieron reestructurar el árbol filogenético para la característica. Con base en el alto número de es (mas) tas nuevas inversiones y otras evidencias se postuló como arreglo ancestral el Tree Line (TL) y se sugiere como centro de origen de la especie la región en que colindan los estados de Michoacán y Zacatecas. Se encontraron gradientes geográficos para algunas inversiones tanto en localidades distantes como micro-geográficas. Un estudio de recolectas semestrales por ocho años con las otras dos especies señaló alto grado de diversidad para la frecuencia de inversiones, 64.3% para D. willistoni y 52.9% para D. nebulosa. En ambas especies se cuantificó el número promedio de inversiones por hembra de 2.57 para D. willistoni y de 1.57 para D. nebulosa, que las sitúan dentro del área marginal de la distribución de la especie. Sin embargo, conservan alto grado de diversidad que aparentemente no les correspondería debido a su marginalidad. Resumen en inglés In Mexico , chromosomal polymorphism in Drosophila has been studied in D. pseudoobscura, D. willistoni and D. nebulosa. The first species is better studied with respect to the number of populations analyzed (close to 60), in which there were recognized up to 27 inversions corresponding to the 67.5 % of the total variability for this characteristic, identifying four races. With all of these evidences we rebuilt phylogenetic tree and postulate as ancestral gene arrangement (mas) the sequence Tree Line (TL) and suggesting as origin center of the species the surrounded area between Michoacán and Zacatecas states. Geographical gradients for several inversions at both macro and micro-geographic scales were also detected. The other species were both studied for a seasonal changes during eight years. As a result, it was found a high variability degree for the inversions number, 64.3% of the total variability for this parameter in D. willistoni and 52.9 % in D. nebulosa. Average number of inversions carried by a female was 2.57 in D. willistoni and 1.57 in D. Nebulosa, which set as marginal with respect to the distribution. Nevertheless, they show a considerable variability not expected for a marginal population.

Scientific Electronic Library Online (Spanish)

47

EVALUACIÓN DE LA ACTIVIDAD BIOLÓGICA DE EMULSIONES ACEITE/AGUA DE ÁCIDO OLEICO-NICOTINA SOBRE Drosophila melanogaster/ BIOLOGICAL ACTIVITY EVALUATION OF OLEIC ACID-NICOTINE EMULSIONS OIL/WATER AGAINST Drosophila melanogaster

ARAQUE M, Pedronel; PELÁEZ J, Carlos A
2010-01-01

Resumen en español En este trabajo se estudia el efecto de las emulsiones O/W de ácido oleico-nicotina estabilizadas con Tween 80® sobre su actividad insecticida aguda y crónica en Drosophila melanogaster. La bioactividad aguda de las emulsiones de ácido oleico-nicotina registró un tiempo letal medio (TL50 = 13 ± 3 minutos) significativamente mayor que el obtenido con la nicotina en solución acuosa (TL50 = 7 ± 1 minutos). La baja bioactividad de la emulsión de ácido oleico-nicotin (mas) a presenta una alta correlación con el porcentaje de nicotina encapsulada en las gotas de emulsión. La bioactividad crónica se evaluó en el ciclo de vida de D. melanogaster, donde se observó repelencia, evidenciada por la disminución en la oviposición. Resumen en inglés This paper studies the effect of the O/W emulsion of oleic acid-nicotine stabilized with Tween 80® on its acute and chronic insecticidal activity in Drosophila melanogaster. The oleic acid-nicotine emulsions acute bioactivity show a median lethal time (LT50 = 13 ± 3 minutes) significantly higher compared with that obtained with nicotine in aqueous solution (LT50 = 7 ± 1 minutes). Oleic acid-nicotine emulsion low bioactivity present high correlation with the percentage (mas) of nicotine encapsulated in emulsion droplets. The chronic bioactivity is assessed in the life cycle of D. melanogaster where diminution in oviposition is taken as evidence of repellency.

Scientific Electronic Library Online (Spanish)

48

Jak/Stat signalling in niche support cells regulates dpp transcription to control germline stem cell maintenance in the Drosophila ovary

López-Onieva, Lourdes; Fernández-Miñán, Ana; González-Reyes, Acaimo

8 pages, 5 figures.-- PMID: 18171682 [PubMed].-- Printed version published Feb 2008.-- Supporting information (Suppl. table S1, figure S1) available at: http://dev.biologists.org/cgi/content/full/135/3/533/DC1 | The existence of specialised regulatory microenvironments or niches that sustain stable ...

DRIVER (Spanish)

51

RNA interference has a role in regulating Drosophila telomeres

Casacuberta, Elena; Pardue, Mary Lou

The electronic version of this article is the complete one and can befound online at http://genomebiology.com/2006/7/5/220 | Unlike many other organisms, Drosophila maintains its telomeres by the transposition ofretrotransposons to chromosome ends. Recent work shows that proteins in the RNAinterf...

DRIVER (Spanish)

53

EVALUACIÓN DE LA ACTIVIDAD BIOLÓGICA DE EXTRACTOS DE Crotalaria Juncea MEDIANTE EL MODELO Drosophila melanogaster/ EVALUATION OF BIOLOGICAL ACTIVITY OF Crotalaria Juncea EXTRACTS THROUGH A Drosophila melanogaster MODEL

PEÑALOZA A, Giann C; PELÁEZ J, Carlos A
2008-07-01

Resumen en español El crecimiento desmesurado de la población mundial ha presionado a la búsqueda de métodos para el control de plagas, de modo tal que sea posible una producción racional y sostenida de alimentos, y adicionalmente, se garantice la conservación de los ecosistemas. Con el modelo biológico Drosophila melanogaster es posible evaluar la actividad de extractos de diferente polaridad obtenidos a partir de Crotalaria juncea (Fabaceae). El modelo facilita la identificación y (mas) purificación de las fracciones activas, y permite establecer que dichos extractos ejercen dos tipos de actividad inhibitoria sobre su ciclo de vida. La primera,se manifiesta con la disminución del número de adultos en los tratamientos frente al control; y la segunda, causa la inhibición de los estadios larvarios del modelo, lo cual se manifiesta en una disminución en el número de pupas. Con el uso de ensayos biodirigidos y de técnicas espectroscópicas de resonancia magnética nuclear (RMN), se establece la estructura de un principio activo que causa los dos tipos de inhibición sobre el modelo, un alcaloide pirrolizidínico del tipo retronécico, conocido como Junceína. El uso del modelo biológico como herramienta analítica cuantitativa, permite establecer la correlación de las variables dosis y respuesta. Resumen en inglés The disproportionate growth of world population has led the search methods to control pests that permit a rational and sustained production of food, which additionally ensure the conservation of ecosystems. The activity of extracts with different polarity obtained from C. juncea was evaluated using the biological model of Drosophila melanogaster. This model allows the identification and purification of active fractions, and allows establishing that these extracts exert tw (mas) o different inhibitory activities over the life cycle of Drosophila melanogaster. The first activity showed the reduction in the number of adults compared with the control group. The second activity caused the inhibition of the larval estate then it resulted in diminution of the pupal number. The use of the biological assay and spectroscopy techniques as Nuclear Magnetic Resonance, allowed the establishment that the structure of active principle of Crotalaria juncea is in agreement with a pyrrolizidinic alkaloid of retronecic type well known as Junceine. The use of biological model as quantitative analytical tool, allows the establishment of correlation between dosage and response variables.

Scientific Electronic Library Online (Spanish)

54

Independent roles of Drosophila Moesin in imaginal disc morphogenesis and hedgehog signalling

Molnar, Cristina; de Celis, Jose F.

Article available at http://dx.doi.org/10.1016/j.mod.2006.02.001 | The three ERM proteins (Ezrin, Radixin and Moesin) form a conserved family required in many developmental processes involving regulation of the cytoskeleton. In general, the molecular function of ERM proteins is to link specific memb...

DRIVER (Spanish)

56

Evaluación de cepas de Bacillus thuringiensis para el control de Drosophila melanogaster Maengi/ Evaluation of Bacillus thuringiensis Strains for the control of Drosophila melanogaster Maengi

Carreras Solís, Bertha; Rodríguez Batista, Dayamí
2009-06-01

Resumen en español El uso de Bacillus thuringiensis como biolarvicida es una alternativa viable para el control de insectos, por lo que el número de cepas activas contra diptera está en ascenso. En el presente trabajo se realizó la caracterización de cepas de esta bacteria aisladas de las diferentes regiones de Cuba, la cual se basó en la actividad insecticida contra Drosophila melanogaster, observación al microscopio electrónico de la mezcla de esporas y cristales, y electroforesis (mas) en gel de poliacrilamida con duodecil sulfato de sodio de la suspensión de esporas-cristales. Las cepas LBT-63 y LBT-87 mostraron similitudes con el B. thuringiensis subsp. israelensis usado en este estudio, en relación con el perfil proteico y la morfología de las inclusiones cristalinas. Estas cepas mostraron proteínas de 130, 70 y 27 kDa y cristales amorfos; sin embargo, solamente la cepa LBT-87 mostró actividad contra D. melanogaster, aunque más baja que el B. thuringiensis subs. israelensis control Resumen en inglés The use of Bacillus thuringiensis as biolarvicide is a viable alternative for insect control so the number of known Bacillus thuringiensis strains active on diptera is growing. The characterization of strains of this bacterium isolated from different regions of Cuba is presented. Characterization was based on their insecticidal activity against Drosophila melanogaster, electron microscopy observation of the spores and crystal mixtures and sodium dodecyl sulfatepolyacrylam (mas) ide gel electrophoresis (SDS-PAGE) of spore-crystal suspensions. Strains LBT-63 and LBT-87 displayed similarities with the B. thuringiensis subs. israelensis used in this study with regard profile protein and morphology of crystal inclusions. These strains had major proteins of 130, 70 and 27 kDa and amorphous crystal; however, the strain LBT-87 only showed insecticidal activity against D.melanogaster but lower than B. thuringiensis subs. israelensis control

Scientific Electronic Library Online (Spanish)

57

Cell surface proteins Nasrat and Polehole stabilize the Torso-like extracellular determinant in Drosophila oogenesis

Jiménez, Gerardo; González-Reyes, Acaimo; Casanova, Jordi

PMCID: PMC152353 | Structural cell-surface and extracellular-matrix proteins modulate intercellular signaling events during development, but how this is achieved remains largely unknown. Here we identify a novel family of Drosophila proteins, Nasrat and Polehole, that coat the oocyte surface and pla...

DRIVER (Spanish)

59

Cloning and developmental expression of Sna, a murine homologue of the Drosophila snail gene

Nieto, M. Ángela; Bennett, Michael F.; Sargent, Michael G.; Wilkinson, David G.
1992-09-01

Digital.CSIC (Spain)

60

Ciclos de vida y componentes de la aptitud de Drosophila inca y D. yangana (Diptera, Drosophilidae)/ Life cycles and fitness components of Drosophila inca and D. yangana (Diptera, Drosophilidae)

Mafla M., Ana Beatriz
2005-03-01

Resumen en español Se describen los ciclos de vida de Drosophila inca Dobzhansky & Pavan, 1943 y D. yangana Rafael & Vela, 2003 los cuales son muy similares; la viabilidad observada es diferente ya que la mortalidad en estagios larvarios y de pupación de D. yangana es superior debido a la alimentación inadecuada. La duración promedia desde huevo hasta adulto es 22 días a 21 - 23°C para ambas especies. Resumen en inglés The life cycles of Drosophila inca Dobzhansky & Pavan, 1943 and D. yangana Rafael & Vela, 2003 are described and proved to be very similar; the viability observed is different since the mortality in the larval and pupal stages of D. yangana is higher because of inadequate feeding. The mean duration from egg to adult is 22 days at 21-23° C for both species.

Scientific Electronic Library Online (Spanish)

61

Dynamic Control of Cell Cycle and Growth Coupling by Ecdysone, EGFR, and PI3K Signaling in Drosophila Histoblasts

Ninov, Nikolay; Manjón, Cristina; Martín-Blanco, Enrique

12 pages, 7 figures.-- PMID: 19355788 [PubMed].-- Supporting information (Suppl. figures S1-S3, movies S1-S7) available at: http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.1000079#toclink6 | Regulation of cell proliferation has been extensively studied in cult...

DRIVER (Spanish)

63

Control of growth and positional information by the graded vestigial expression pattern in the wing of Drosophila melanogaster

Baena, Luis Alberto; García-Bellido, Antonio

The size and shape of organs depend on cellular processes such as cell proliferation, cell survival, and spatial arrangement of cells. In turn, all of these processes are a consequence of positional identity of individual cells in whole organs. Links of positional information with organ growth and p...

DRIVER (Spanish)

65

Specific interactions of chromatin with the nuclear envelope: positional determination within the nucleus in Drosophila melanogaster

Marshall, Wallace F.; Dernburg, Abby F.; Harmon, Brian; Agard, David A.; Sedat, John W.
1996-05-01

Digital.CSIC (Spain)

66

Análisis funcional del gen nab en el desarrollo de drosophila melanogaster

Terriente Félix, Javier

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 15-12-2006 | Nab proteins form an evolutionarily conserved family of transcriptional co-regulators implicated in multiple developmental events in various org...

DRIVER (Spanish)

67

Análisis funcional del gen nab en el desarrollo de drosophila melanogaster

Jiménez Diaz-Benjumea, Fernando; Terriente Félix, Javier
2006-01-01

Digital.CSIC (Spain)

68

The GAGA factor of Drosophila binds triple-stranded DNA

Jiménez García, Emilio; Vaquero, Alejandro; Espinás, María Luisa; Soliva, Robert; Orozco, Modesto; Bernués, Jordi; Azorín, Ferran
1998-09-18

Digital.CSIC (Spain)

69

Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton

Bolívar, Jorge; Huynh, Jean-René; López-Schier, Hernán; González, Cayetano; St Johnston, Daniel; González-Reyes, Acaimo

During early Drosophila oogenesis, one cell from a cyst of 16 germ cells is selected to become the oocyte, and accumulates oocyte-specific proteins and the centrosomes from the other 15 cells. Here we show that the microtubule cytoskeleton and the centrosomes follow the same stepwise restriction to ...

DRIVER (Spanish)

70

Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton

Bolívar, Jorge; Huynh, Jean-René; López-Schier, Hernán; González, Cayetano; St Johnston, Daniel; González-Reyes, Acaimo
2001-04-19

Digital.CSIC (Spain)

71

EVALUACIÓN DE DOS MEDIOS DE CULTIVO Y HEREDABILIDAD DE PRODUCTIVIDAD Y TIEMPO DE DESARROLLO PARA TRES MUTANTES DE Drosophila melanogaster (DROSOPHILIDAE)/ Evaluation of Two Culture Media and Heritability of Productivity and Development Time for Three Mutants of Drosophila melanogaster (Drosophilidae)

DÍAZ-GONZÁLEZ, FERNANDO; PIZARRO-LOAIZA, MAYRA; CASTRILLÓN, MAURICIO RAMÍREZ; MOLINA-HENAO, YHERSON; SOLARTE-GARCÍA, DIEGO; BRAVO-GUERRERO, DANIELA; HURTADO-GIRALDO, ALEJANDRA; CÁRDENAS-HENAO, HEIBER
2008-04-01

Resumen en español Con el objetivo de investigar el efecto del medio de cultivo en la productividad y tiempo de desarrollo huevo-adulto de una cepa silvestre y tres cepas mutantes (CyLv, vg, w) de Drosophila melanogaster, se examinaron dos tipos de medios: banano y naranja. Para esto se empleó un diseño con dos factores, medio de cultivo y tipo de cepa, para un total de ocho tratamientos con cinco repeticiones cada uno. Se obtuvo que la productividad y el tiempo de desarrollo dependen del (mas) medio de cultivo y el tipo de cepa, encontrándose mayor productividad en el medio de naranja. La cepa silvestre presentó la mayor productividad y el menor tiempo de desarrollo en los dos medios (α=0,05). El análisis genético evidenció una heredabilidad baja y una variación fenotípica debida en su mayor parte al componente de interacción genotipo-ambiente, lo que explica la diferencia en el patrón de productividad y tiempo de desarrollo entre medios de cultivo. Resumen en inglés With the objective of investigating the effect of the culture media in the productivity and development time egg-adult of Drosophila melanogaster in +/+ and three mutants (CyLv, vg, w), two culture media: banana and orange, were evaluated. An experimental design with two factors: culture media and kind of flies-stock, were tested, for a total of eight treatments with five replicas each one. The productivity and development time depend on culture media and kind of flies-st (mas) ock, and the biggest productivity was in the orange culture media. The +/+ presented the biggest productivity and lowest development time in both culture media (α=0.05). The genetic analysis showed a low heritability and the phenotypic variation was due, in a mayor part, to the component of the interaction genotype-environment that explains the difference in the patron of productivity and development time between culture media.

Scientific Electronic Library Online (Spanish)

72

Análisis electroforético de las relaciones evolutivas en las especies de Drosophila (Diptera: Drosophilidae) del enjambre martensis: D. martensis, D. starmeri y D. uniseta/ Electrophoretic analysis of the evolutionary relations in Drosophila species (Diptera: Drosophilidae) of the cluster martensis: D. martensis, D.starmeri and D. uniseta

ORDÓÑEZ, MARINA; ARENAS, ANGÉLICA; BETANCOURT, LUZ ANGELA; DE POLANCO, MARÍA MAGDALENA
2004-06-01

Resumen en español En geles de almidón fue inferida electroforéticamente la variación genética de doce loci isoenzimáticos, entre seis poblaciones alopátricas colombianas del enjambre martensis (D.martensis, D. starmeri y D. uniseta). provenientes de Santa Marta (departamento del Magdalena), Camarones (departamento de la Guajira) y del desierto de la Tatacoa (departamento del Huiia). Esta estimación fue confrontada con una especie filogenéticamente relacionada (D. buzzatii) y una di (mas) stante (D. melanogaster). Las divergencias enzimáticas se infirieron a partir de distancias genéticas y los resultados permitieron identificar seis niveles de divergencia taxonómica: Población local (D = 0,1031), subrazas (D = 0,1412), subespecies o razas (D = 0,1870), especies de un mismo enjambre o cluster (D = 0,7907), especies de enjambres diferentes (D = 1,0055) y especies de grupos diferentes (D = 1,4327). A través de este estudio preliminar, se confirmó la utilidad de los datos electroforéticos como estimadores de divergencia evolutiva y se sugiere un posible ejemplo de especiación alopátrica. Resumen en inglés Starch gel electrophoresis was used to elucídate genetic variation at 12 isoenzyme loci among six allopatric Colombian populations of the cluster martensis (D. martensis, D. starmeri and D. uniseta), from Santa Marta (Magdalena Department), Camarones (Guajira Department) and from the Tatacoa desert (Huila Department). This estimation was compared to a phylogenetically related (D. buzzatii) and distant (D. melanogaster) species. The enzymatic divergences were inferred fro (mas) m genetic distantes and the results allowed the identification of six levéis of taxonomic divergence: Local population (D = 0,1031), subraces (D = 0,1412), subspecies or races (D = 0,1870), species of a same cluster (D = 0,7907), species of different clusters (D = 1,0055) and species from different groups (D = 1,4327). Through this preliminary study, the utility of electrophoretic data as estimators of evolutionary divergence was confirmed and a possible example of allopatric speciation is suggested.

Scientific Electronic Library Online (Spanish)

73

Caracterización funcional del gen "vertigial" de "Drosophila melanogaster"

Baena, Luis Alberto

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 24-02-2006 | The development of Drosophila imaginal discs is a classical model to study the growthand genetic specification of growing organs. The growth, s...

DRIVER (Spanish)

75

EFECTO DE DENSIDAD POBLACIONAL DE HUEVOS SOBRE VIABILIDAD Y TIEMPO DE DESARROLLO DE Drosophila melanogaster (DROSOPHILIDAE)/ Effect Of Eggs Population Density On Viability And Time Of Development Of Drosophila melanogaster (Drosophilidae)

FONG, CRISTIAN; DÍAZ, FERNANDO; OSORIO, JULIO; CASTANO, LINA; GONZÁLEZ, FANNY; JURADO, LAURA; CASTILLO, KATHERINE; CÁRDENAS, HEIBER
2008-08-01

Resumen en español Para estimar el efecto de la densidad poblacional de huevos sobre la viabilidad huevoadulto de Drosophila melanogaster se realizaron tres tratamientos en frascos con medio de cultivo agar-banano: a densidad de 10, 50 y 90 huevos. La variable de respuesta fue la proporción de individuos adultos emergidos desde la aparición del primer imago hasta 15 días después de la siembra. Las viabilidades huevo-adulto promedio fueron 0,320, 0,338 y 0,328 para las densidades de 10, (mas) 50 y 90 huevos respectivamente. No se evidenciaron diferencias significativas entre los tres tratamientos (p>0.05). Por lo tanto, no se detectó en este estudio influencia de la densidad poblacional de huevos sobre la viabilidad huevo-adulto. Probablemente debido a que la proporción entre el número de huevos y la cantidad de medio fue suficientemente alta para no generar competencia en otros estadios del desarrollo huevo/adulto. Resumen en inglés For estimating the effect from population density of eggs over viability egg-adult in Drosophila melanogaster, it was made three treatments in flasks with agar-banana culture media: densities of 10, 50 and 90 eggs. The effect evaluated was the adult’s proportion that emerged after 15 days of planting. The mean proportions of viability egg-adult were 0.32, 0.338 and 0.328 for the density of 10, 50 and 90 eggs respectively. Significant differences in viability were not evi (mas) dent (p>0.05). Consequently, it was not found effects from the eggs densities in the present study over egg-adult viability. These results probably were due to that the relation among number of eggs and quantity of culture media was not enough of a high for generate competition at another stadiums through the egg-adult development.

Scientific Electronic Library Online (Spanish)

76

Función del gen dRYBP en "Drosophila melanogaster"

González, Inmaculada

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura 23-03-2007 | The Polycomb (PcG) and trithorax (trxG) groups of genes are involved in the mechanismsof maintenance of gene expression during development. The ...

DRIVER (Spanish)

77

Función del gen dRYBP en "Drosophila melanogaster"

Busturia, Ana; González González, María Inmaculada
2007-01-01

Digital.CSIC (Spain)

79

Selective maintenance of Drosophila tandemly arranged duplicated genes during evolution

Quijano, Carlos; Tomancak, Pavel; López-Marti, Jesús; Suyama, Mikita; Bork, Peer; Milán, Marco; Torrents, David

[Background] The physical organization and chromosomal localization of genes within genomes isknown to play an important role in their function. Most genes arise by duplication and move alongthe genome by random shuffling of DNA segments. Higher order structuring of the genome occursin eukaryotes...

DRIVER (Spanish)

80

Selective maintenance of Drosophila tandemly arranged duplicated genes during evolution

Quijano, Carlos; Tomancak, Pavel; López-Marti, Jesús; Suyama, Mikita; Bork, Peer; Milán, Marco; Torrents, David; Manzanares, Miguel
2008-12-16

Digital.CSIC (Spain)

82

EYS, encoding an ortholog of Drosophila spacemaker, is mutated in autosomal recessive retinitis pigmentosa

Abd El-Aziz, Mai M.; Barragán, Isabel; O'Driscoll, Ciara A.; Goodstadt, Leo; Prigmore, Elena; Borrego, Salud

3 pages, 2 figures, 1 table.-- PMID: 18836446 [PubMed].-- Supporting information (Suppl. Methods, Suppl. Note, Suppl. Figs 1–4, Suppl. Table 1) available at: http://www.nature.com/ng/journal/v40/n11/suppinfo/ng.241_S1.html | Using a positional cloning approach supported by comparative genomics, we h...

DRIVER (Spanish)

83

EYS, encoding an ortholog of Drosophila spacemaker, is mutated in autosomal recessive retinitis pigmentosa

Abd El-Aziz, Mai M.; Barragán, Isabel; O'Driscoll, Ciara A.; Goodstadt, Leo; Prigmore, Elena; Borrego, Salud; Mena, Marcela; Pieras, Juan I.; El-Ashry, Mohamed F.; Abu Safieh, Leen; Shah, Amna; Cheetham, Michael E.; Carter, Nigel P.; Chakarova, Christina; Ponting, Chris P.; Bhattacharya, Shomi S.; Antiñolo, Guillermo
2008-10-05

Digital.CSIC (Spain)

84

Gene Expression Variations During Drosophila Metamorphosis in Space. The GENE Experiment in the Spanish Cervantes Mission to the ISS

Herranz, Raul; Benguria, Alberto; Fernandez-Pineda, Emilio; Medina, F. Javier; Gasset, Gilbert; van Loon, Jack; Zaballos, Angel; Marco, Roberto
2005-01-01

Digital.CSIC (Spain)

86

Apposition of iroquois expressing and non-expressing cells leads to cell sorting and fold formation in the Drosophila imaginal wing disc

Villa-Cuesta, Eugenia; González-Pérez, Esther; Modolell, Juan

This article is available from: http://www.biomedcentral.com/1471-213X/7/106 | [Background] The organization of the different tissues of an animal requires mechanisms thatregulate cell-cell adhesion to promote and maintain the physical separation of adjacent cellpopulations. In the Drosophila imag...

DRIVER (Spanish)

88

Análisis morfométrico de la pigmentación en especies colombianas de Drosophila (Diptera: Drosophilidae) del grupo repleta/ Morphometric analysis of the pigmentation in colombian species of Drosophila (Diptera: Drosophilidae) from the repleta group

PRIETO S, RODRIGO; E. DE POLANCO, MARÍA MAGDALENA
2009-12-01

Resumen en español Se comparó la pigmentación abdominal de poblaciones aisladas de las especies del grupo repleta: Drosophila martensis, D. starmeri y D. uniseta (cluster martensis). El propósito fue detectar si la distancia que las separa, aunada a la heterogeneidad ambiental, están relacionadas con un proceso de formación de razas geográficas. De otra parte, se comparó la variación del mencionado carácter en D. aldrichi, del conglomerado mulleri, con la de las especies del conglo (mas) merado martensis, tratando de contrastar las relaciones evolutivas entre estas especies. Se digitalizó el contorno de la tercera mancha abdominal con descriptores elípticos de Fourier y análisis canónicos multivariados en las cuatro especies recolectadas en las poblaciones de Camarones (Riohacha-Guajira), Santa Marta (Magdalena) y Rosalía (Desierto de la Tatacoa, Huila). Los resultados mostraron diferencias significativas en la forma de la mancha abdominal entre las especies (P Resumen en inglés The abdominal pigmentation of geographically isolated populations of species of the repleta group, D. martensis, D. starmeri and D. uniseta ( martensis cluster), was compared. Our purpose was to detect whether the distance that separated them, together with the environmental heterogeneity, are related to a process of geographic race formation. Moreover, variation in the aforementioned character was compared between D. aldrichi from the mulleri cluster, and species from th (mas) e martensis-cluster species, attempting to contrast the evolutionary relationships among these species. The outline of the third-abdominal spot was digitalized with elliptic Fourier descriptors and multivariate canonical analysis for the four populations collected from Camarones (Riohacha-Guajira Department), Santa Marta (Magdalena Department) and Rosalía (Tatacoa Desert, Huila Department). Results showed significant differences in the form of the abdominal spot morphology among the species (P

Scientific Electronic Library Online (Spanish)

89

Sharp boundaries of Dpp signalling trigger local cell death required for Drosophila leg morphogenesis

Manjón, Cristina; Sánchez-Herrero, Ernesto; Suzanne, Magali

Article available at http://dx.doi.org/10.1038/ncb1518 | Morphogens are secreted signalling molecules that govern many developmental processes1. In the Drosophila wing disc, the transforming growth factor (TGF) homologue Decapentaplegic (Dpp) forms a smooth gradient and specifies cell fate by confe...

DRIVER (Spanish)

91

Overexpression of troponin T in Drosophila muscles causes a decrease in the levels of thin-filament proteins

Marco-Ferreres, Raquel; Arredondo, Juan J.; Fraile, Benito; Cervera, Margarita
2005-02-15

Digital.CSIC (Spain)

92

The Ultrabithorax Hox gene of Drosophila controls haltere size by regulating the Dpp pathway

Navas, Luis F. de; Garaulet, Daniel L.; Sánchez-Herrero, Ernesto

Article available at http://dx.doi.org/10.1242/dev.02609 | The halteres and wings of Drosophila are homologous thoracic appendages, which share common positional information provided by signaling pathways. The activity in the haltere discs of the Ultrabithorax (Ubx) Hox gene establishes the differen...

DRIVER (Spanish)

93

The Ultrabithorax Hox gene of Drosophila controls haltere size by regulating the Dpp pathway

Navas, Luis de; Garaulet, Daniel L.; Sánchez-Herrero, Ernesto
2006-10-18

Digital.CSIC (Spain)

95

Regulation of spalt expression in the Drosophila wing blade in response to the Decapentaplegic signaling pathway

Barrio, Rosa; de Celis, Jose F.

Pattern formation depends on the acquisition of precise cellular identities due to the differential expression of transcription factors. Enhancers within regulatory regions integrate the positive and negative regulatory signals directing gene transcription. Here, we analyze the enhancer that drives ...

DRIVER (Spanish)

99

A sequence motif enriched in regions bound by the Drosophila dosage compensation complex

Gallach, Miguel; Arnau, Vicente; Aldecoa, Rodrigo; Marín, Ignacio
2010-03-12

Digital.CSIC (Spain)

100

Restriction Map Variation at the Adh Locus of Drosophila Melanogaster in Inverted and Noninverted Chromosomes

Aguade, M.

Restriction map variation among 39 Standard and 40 In(2L)t chromosomes extracted from a Spanish natural population of Drosophila melanogaster was investigated for a 2.7-kb region encompassing the Adh locus with ten four-cutter restriction enzymes. A total of 20 polymorphisms were detected, represent...

DRIVER (Spanish)

101

Regulation of decapentaplegic expression during Drosophila wing veins pupal development

Sotillos, Sol; de Celis, Jose F.

Article available at http://dx.doi.org/10.1016/j.mod.2005.12.002 | The differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during pupal development. The expression of dpp in the pupal veins requires the integrity of the shor...

DRIVER (Spanish)

103

Trans regulation in the Ultrabithorax gene of Drosophila: alterations in the promoter enhance transvection

Martínez-Laborda, Antonio; González-Reyes, Acaimo; Morata, Ginés

PMCID: PMC556824 | We report a genetic and molecular study of UbxMX6 and Ubx195rx1, two mutations in the Ultrabithorax (Ubx) locus which appear to have a strong effect on the activity of the homologous Ubx gene. These mutations show the characteristic embryonic and adult phenotypes of Ubx null allel...

DRIVER (Spanish)

104
105

Polarization of both major body axes in Drosophila by gurken-torpedo signalling

González-Reyes, Acaimo; Elliott, Heather; St Johnston, Daniel

Final full-text version of this paper available at: http://dx.doi.org/10.1038/375654a0 | Anterior–posterior polarity in Drosophila arises from the movement of the oocyte to the posterior of the egg chamber, and the subsequent acquisition of posterior fate by the adjacent somatic follicle cells. We d...

DRIVER (Spanish)

106

Polarization of both major body axes in Drosophila by gurken-torpedo signalling

González-Reyes, Acaimo; Elliott, Heather; St Johnston, Daniel
1995-06-22

Digital.CSIC (Spain)

107

Requirement of abdominal-A and Abdominal-B in the developing genitalia of Drosophila breaks the posterior downregulation rule

Foronda, David; Estrada, Beatriz; de Navas, Luis; Sánchez-Herrero, Ernesto

Article available at http://dx.doi.org/10.1242/dev.02173 | The genitalia of Drosophila derive from the genital disc and require the activity of the Abdominal-B (Abd-B) Hox gene. This gene encodes two different proteins, Abd-B M and Abd-B R. We show here that the embryonic genital disc, like the larv...

DRIVER (Spanish)

109

Integrin Signaling Regulates Spindle Orientation in Drosophila to Preserve the Follicular-Epithelium Monolayer

Fernández-Miñán, Ana; Martín-Bermudo, María D.; González-Reyes, Acaimo

Final full-text version of the paper available at: http://dx.doi.org/10.1016/j.cub.2007.02.052 | Epithelia act as important physiological barriers and as structural components of tissues and organs. In the Drosophila ovary, follicle cells envelop the germline cysts to form a monolayer epithelium. Du...

DRIVER (Spanish)

110

Integrin Signaling Regulates Spindle Orientation in Drosophila to Preserve the Follicular-Epithelium Monolayer

Fernández-Miñán, Ana; Martín-Bermudo, María D.; González-Reyes, Acaimo
2007-04-17

Digital.CSIC (Spain)

111

The Complex Spatio-Temporal Regulation of the Drosophila Myoblast Attractant Gene duf/kirre

Guruharsha, K.G.; Ruiz-Gómez, Mar; Ranganath, H.A.; Siddharthan, Rahul; VijayRaghavan, K.
2009-09-09

Digital.CSIC (Spain)

112

Modifications in basic handling techniques to study the consequences of the Drosophila melanogaster exposure to the space environment

Herranz, Raul; Husson, David; Villa, Aida; Pastor, Miquel; Medina, F. Javier; Marco, Roberto
2005-01-01

Digital.CSIC (Spain)

113

Design and development of hardware for long term cultivation of Drosophila melanogaster in the International Space Station

Husson, David; Herranz, Raul; Villa, Aida; Diaz, Carlos; Mateos, Jesus; Lesher, J.; Pastor, Miquel; Medina, F. Javier; Marco, Roberto
2004-12-01

Digital.CSIC (Spain)

114

Comparative genomic analysis of the odorant-binding protein family in 12 Drosophila genomes: purifying selection and birth-and-death evolution

Vieira, Filipe G.; Rozas, Julio; Sánchez-Gracia, Alejandro

The electronic version of this article is the complete one and can befound online at http://genomebiology.com/2007/8/11/R235 | [Background] Chemoreception is a widespread mechanism that is involved in critical biologicprocesses, including individual and social behavior. The insect peripheral olfac...

DRIVER (Spanish)

116

Egalitarian and the case of the missing link

Pearson, John; González-Reyes, Acaimo

Final full-text version of the paper available at: http://dx.doi.org/10.1038/ncb0504-381 | The specificity of cytoskeleton-mediated cargo transport is critical for cell polarization. The Egalitarian protein — required for the localization of mRNAs and proteins to the oocyte of the Drosophila melanog...

DRIVER (Spanish)

117

Regulation of growth by ploidy in Caenorhabditis elegans

Lozano, Encarnación; Sáez, Alberto G.; Flemming, Anthony J.; Cunha, Ana; Leroi, Armand M.

6 pages, 4 figures, 2 tables.-- PMID: 16527744 [PubMed]. | Some animals, such as the larvae of Drosophila melanogaster, the larvae of the Appendicularian chordate Oikopleura, and the adults of the nematode Caenorhabditis elegans, are unusual in that they grow largely by increases in cell size. The g...

DRIVER (Spanish)

118

Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections

Tenllado, Francisco; Martínez-García, Belén; Vargas, Marisol; Díaz-Ruíz, José Ramón

[Background] Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in a diverse group of organisms that includes plants, Caenorhabditis elegans, Drosophila and mammals. We have previously shown and patented that mechanical inoculation of in vitro-transcribed dsRNA derived from viral se...

DRIVER (Spanish)

119

Capacidad protectora de Cymbopogon citratus (DC.) Stapf: ante el daño genético inducido por estrés oxidativo

Cápiro, Nancy; Sánchez-Lamar, Ángel; Fonseca, Gladys; Baluja, Ligia; Borges, Ernesto
2001-03-01

Resumen en español Se estudió el efecto protector de una decocción de las hojas de la planta Cymbopogon citratus (DC.) Stapf. (caña santa o caña de limón) frente al daño genético inducido por los agentes oxidantes H2O2 y etilnitrosourea (ENU). Se utilizó para ello un modelo esperimental in vitro y otro in vivo: cultivo de células de ovario de Hamster chino (CHO) y el insecto Drosophila melanogaster, respectivamente. La capacidad de formar colonias de la línea celular CHO, fue el i (mas) ndicador de toxicidad utilizado para evaluar 5 concentraciones (0,1; 1; 5; 10; 15 %) de la decocción de caña santa, en tanto que el porcentaje de sobrevivencia de adultos se usó para evaluar el efecto tóxico de 4 concentraciones de esta (5; 25; 50; 100 %) sobre las larvas mwh + / + flr³ de Drosophila melanogaster; en ambos casos los resultados evidenciaron efectos tóxicos dependientes de las concentraciones utilizadas. Los estudios citofluorimétricos con diclorofluoresceína-diacetato en células CHO permitieron demostrar la capacidad antioxidante de la decocción de caña santa. Dosis no tóxicas de 0,1; 1 y 5 % produjeron reducciones significativas del estrés oxidativo causado por el H2O2. La genotoxicidad y antigenotoxicidad de diferentes concentraciones de la decocción fueron evaluadas mediante los ensayos de aberraciones cromosómicas en células CHO y el SMART de alas de Drosophila melanogaster. Los resultados de actividad mutagénica obtenidos fueron negativos, en tanto que los correspondientes a la acción protectora de las concentraciones probadas reafirmaron la capacidad antioxidante mostrada por el extracto vegetal Resumen en inglés The protective effect of a decoction of Cymbopogon citratus (DC) Stapf. against genetic damage induced by oxidizing agents H2O2 and ethylnitrosourea was studied. To this end, was used an experimental model in vitro and another in vivo; Chinese Hamster ovary cell culture and Drosophila melanogarster respectively. The colony-forming capacity of CHO cell line was the toxicity indicator used to evaluate 5 Cymbopogon citratus (DC) Stap. decoction concentrations (0,1; 1; 5; 10; (mas) 15 %) whereas the percentage of surviving adult insects was used to asses the toxic effect of 4 concentrations of this extract (5; 25; 50; 100 %) on mwh + 1 + flr³ larvae of Drosophila melanogaster. In both cases, the results revealed concentration dependent toxic effects. The cytofluourimetric studies performed on CHO cells with dischlorofluorescein-diacetate showed the antioxidant capacity of the decoction. Non-toxic doses of 0,1; 1 and 5 % caused significant reductions of H2O2 induced oxidative stress. Genotoxicity and antigenotoxicity of the different decoction concentrations were evaluated by chromosomal aberration detection tests in CHO cell and the SMART of Drosophila melanogastes' wings. The results of the mutagenic activity were negative whereas those of the protective capacity of the tested concentrations confirmed the antioxidant capacity of this vegetal extract

Scientific Electronic Library Online (Spanish)

120

Inventario taxonómico de drosophilidae (Diptera) en el Parque Nacional Yasuni, Amazonia Ecuatoriana/ Taxonomic survey of drosophilidae (Diptera) in the Yasuni National Park, Ecuadorian Amazon

Acurio, Andrea Elizabeth; Rafael, Violeta Lily
2009-09-01

Resumen en español En el Parque Nacional Yasuní, reconocido como un sector de alto endemismo y biodiversidad, ubicado al noroeste de la Amazonía Ecuatoriana se realizó el inventario taxonómico de la familia Drosophilidae. Para la captura de los individuos se utilizaron trampas con atrayente de banano y solución de levadura de cerveza. La identificación taxonómica se realizó usando caracteres morfológicos y la terminalia de los machos. En total se colectaron 7425 individuos clasific (mas) ados en 34 especies de los géneros: Drosophila Fallén, 1823, Scaptodrosophila Duda, 1923, Neotanygastrella Duda, 1923 y Zaprionus Coquillett, 1901. Seis de estas especies son nuevos registros para el Ecuador: D. araicas Pavan & Nacrur, 1950, D. equinoxialis Dobzhansky, 1946, D. kikkawai Burla, 1954, D. subsaltans Magalhães, 1956, D. neocordata Magalhães, 1956 y D. peninsularis Patterson & Wheeler, 1942. Estos datos incrementan el número de especies registrados para el país y para la región amazónica. Resumen en inglés In the Yasuni National Park, a place recognized as a hot spot biodiversity, located in the Northwestern Ecuadorian Amazon was made a taxonomic survey of the Drosophilidae. Individuals were collected using traps with banana and yeast as bait. Taxonomic identifications were made by morphologic characters and male genitalia analysis. We collected 7425 individuals of 34 species, from the genera: Drosophila Fallén, 1823, Scaptodrosophila Duda, 1923, Neotanygastrella Duda, 192 (mas) 3 y Zaprionus Coquillett, 1901. Six of them are new records for Ecuador: D. araicas Pavan & Nacrur, 1950, D. equinoxialis Dobzhansky, 1946, D. kikkawai Burla, 1954, D. subsaltans Magalhães, 1956, D. neocordata Magalhães, 1956 and D. peninsularis Patterson & Wheeler, 1942. This data increase the number of species records to Ecuador and the Amazon Region.

Scientific Electronic Library Online (Spanish)

121

Redescripción y algunos aspectos ecológicos de Girardia tigrina, G. cameliae y G. paramensis (Dugesiidae, Tricladida) en Antioquia, Colombia/ Redescription and some ecological aspects of Girardia tigrina, G. cameliae y G. paramensis (Dugesiidae, Tricladida) in Antioquia, Colombia

Muñoz, Mauricio A; Vélez, Imelda
2007-12-01

Resumen en español Turbellaria está integrada por 2 órdenes: Catenulida y Rhabditophora. Dentro del último, el suborden Paludicola comprende las familias Dendrocoelidae, Dugesiidae y Planariidae. En este estudio fueron encontradas: Girardia cameliae, G. paramensis y G. tigrina (Dugesiidae). Fueron analizados 22 sistemas acuáticos (captura manual en zonas de remanso) del centro y sur oriente de Antioquia. En 3 individuos por especie, se hizo descripción de: ancho, longitud y color corpo (mas) ral; forma y tamaño de cabeza; posición auricular; distancia entre manchas oculares; longitud y posición de faringe. Se efectuó análisis histológico de: gonoporo; bulbo, papila, glándulas y lúmen peneal; vesículas seminales y vasos deferentes; atrio masculino; testículos; canal bursal y bolsa copuladora; ovario y oviductos; glándulas de la cáscara. Se midieron: oxigeno disuelto, pH, conductividad y dureza en el agua. Se realizó reconstrucción gráfica de órganos reproductivos (caracteres taxonómicos) en cortes histológicos seriados. G. tigrina y G. paramensis, presentaron amplia distribución geográfica. Estas especies consumieron ex situ: Daphnia pulex, Chironomus sp., Culex sp. y Drosophila sp. (estados larvales). Tales planarias prefirieron aguas clase II y III (según BMWP/Col). Los aspectos físicos y químicos no exhibieron diferencias entre las preferencias de las especies. G. cameliae se encontró en una sola localidad en condiciones particulares. Resumen en inglés The Turbellaria comprises 2 orders: Catenulida and Rhabditophora. In the latter, the suborder Paludicola contains the families Dendrocoelidae, Dugesiidae and Planariidae. In this study, we found Girardia cameliae, Girardia paramensis and Giardia tigrina (Dugesiidae) in 22 aquatic systems using manual capture in river ponds zones of central and southeastern Antioquia. In 3 individuals per species, a description of: corporal color, length, and wide; head form and size; auri (mas) cles position; intraocular distance; pharynx length and position, was made. A histological analysis of gonopore; penis bulb, papilla, glands and lumen; seminal vesicles and vas deferens; masculine atrium; testes; bursal canal and copulatory bursa; ovary and oviducts; shell gland, was also conducted. Water oxygen content, pH, conductivity, and hardness, were measured. The graphical reconstruction of the reproductive organs (taxonomical characters) on serial histological cuts was made. G. tigrina and G. paramensis present a broad geographical distribution. These species ate ex-situ: Daphnia pulex, Chironomus sp., Culex sp. and Drosophila sp. (larval stages). These flatworms prefer waters (BMWP/Col index) II - III water class. The physical and chemical aspects did not exhibit differences between G. tigrina and G. paramensis. G. cameliae was found in one only locality with unusual conditions.

Scientific Electronic Library Online (Spanish)

122
124

Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family

Eberhard, Eberhard; Jiménez, Gerardo; Heavey, Barry; Busslinger, Meinrad
2000-05-15

Digital.CSIC (Spain)

126

The “Gene” Experiment in the Spanish Soyuz Mission to the International Space Station. Effects of cold transportation

Herranz, Raul; Lavan, D. A.; Benguria, Alberto; Duque, Pedro; Zaballos, A.; Medina, F. Javier; Van Loon, Jack; Marco, Roberto; Gasset, Gilbert; Leandro, L. J.
2007-01-01

Digital.CSIC (Spain)

127

The “AGEING” Experiment in the Spanish Soyuz Mission to the International Space Station

de Juan, Emilio; Benguria, Alberto; Villa, Aida; Leandro, L. J.; Herranz, Raul; Duque, Pedro; Horn, Eberhard; Medina, F. Javier; van Loon, Jack; Marco, Roberto
2007-01-01

Digital.CSIC (Spain)

128

The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm.

Weavers, Helen; Prieto-Sánchez, Silvia; Grawe, Ferdinand; Garcia-López, Amparo; Artero, Ruben; Wilsch-Braeuninger, Michaela; Ruiz-Gómez, Mar; Skaer, Helen; Denholm, Barry
2009-01-15

Digital.CSIC (Spain)

129

The gene transformer of anastrepha fruit flies (Diptera, tephritidae) and its evolution in insects

Ruiz Lorenzo, María Fernanda; Milano, Andreina; Salvemini, Marco; Eirín-López, José María; Perondini, André L. P.; Selivon, Denise; Polito, Catello; Saccone, Giuseppe; Sánchez Rodríguez, Lucas
2007-11-28

Digital.CSIC (Spain)

130

The experiments Gene and Ageing performed in the International Space Station (ISS) during the Spanish Soyuz Mission

Marco, Roberto; Herranz, Raul; Villa, Aida; Kirchnick, U.; Horn, Eberhard; Gasset, Gilbert; Agricola, H. J.; van Loon, J.; Medina, F. Javier
2004-01-01

Digital.CSIC (Spain)

131

The cell biology of Smo signalling and its relationships with GPCRs

Ruiz-Gómez, Ana; Molnar, Cristina; Holguín, Helena; Mayor Menéndez, Federico; de Celis, Jose F.
2006-09-30

Digital.CSIC (Spain)

133

The N-terminal POZ domain of GAGA mediates the formation of oligomers that bind DNA with high affinity and specificity

Espinás, María Luisa; Jiménez García, Emilio; Vaquero, Alejandro; Canudas, Silvia; Bernués, Jordi; Azorín, Ferran
1999-06-04

Digital.CSIC (Spain)

135

Regulation of growth by ploidy in Caenorhabditis elegans

Lozano, Encarnación; Sáez, Alberto G.; Flemming, Anthony J.; Cunha, Ana; Leroi, Armand M.
2006-03-07

Digital.CSIC (Spain)

138

Phylogenetic conservation of the regulatory and functional properties of the Vav oncoprotein family

Couceiro, José R.; Martín-Bermudo, María D.; Bustelo, Xosé R.

Vav proteins are phosphorylation-dependent GDP/GTP exchange factors for Rho/Rac GTPases.Despite intense characterization of mammalian Vav proteins both biochemically and genetically,there is little information regarding the conservation of their biological properties in lower organisms.To approac...

DRIVER (Spanish)

139

Phylogenetic conservation of the regulatory and functional properties of the Vav oncoprotein family

Couceiro, José R.; Martín-Bermudo, María D.; Bustelo, Xosé R.
2005-08-15

Digital.CSIC (Spain)

140

Para qué tantas sinapsis? | Why so many synapses?

Ferrús, Alberto

Precede al título: Revisiones en neurociencias. Editor: J.V. Sánchez-Andrés. | [EN]Introduction and development. Synapses are the most abundant structures in the body of all animals. This numberappears to be exceedingly large considering their apparent function: excite or inhibit the postsynaptic c...

DRIVER (Spanish)

142

Mode of action of the plant-derived silphinenes on insect and mammalian GABA(A) receptor/chloride channel complex

Bloomquist, Jeffrey R.; Boina, Dhana Raj; Chow, Ella; Carlier, Paul R.; Reina, Matías; González-Coloma, Azucena
2008-05-01

Digital.CSIC (Spain)

143

Lines is required for normal operation of Wingless, Hedgehog and Notch pathways during wing development.

Benítez, Elvira; Bray, Sarah J.; Rodríguez Enríquez, Isabel; Guerrero Vega, Isabel
2009-04-01

Digital.CSIC (Spain)

144

Isolation and characterization of casein kinase I from Dictyostelium discoideum

Moreno-Bueno, Gema; Calés Bourdet, Carmela; Behrens, M. Margarita; Fernández-Renart, Margarita
2000-07-15

Digital.CSIC (Spain)

145

Hedgehog lipid modifications are required for Hedgehog stabilization in the extracellular matrix

Callejo, Ainhoa; Torroja, Carlos; Quijada, Luis; Guerrero, Isabel

Article available http://dx.dpi.org/10.1242/dev.02217 | The Hedgehog (Hh) family of morphogenetic proteins has important instructional roles in metazoan development. Despite Hh being modified by Ct-cholesterol and Nt-palmitate adducts, Hh migrates far from its site of synthesis and programs cellular...

DRIVER (Spanish)

146

Hedgehog lipid modifications are required for Hedgehog stabilization in the extracellular matrix

Callejo, Ainhoa; Torroja, Carlos; Quijada, Luis; Guerrero Vega, Isabel
2006-01-05

Digital.CSIC (Spain)

148

Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity

Giraldo, Patricia; Martínez, Antonio; Regales, Lucía; Lavado, Alfonso J.; García-Díaz, Ángel; Alonso, Ángel; Busturia, Ana; Montoliu, Lluís
2003-11-01

Digital.CSIC (Spain)

149

Egalitarian and the case of the missing link

Pearson, John; González-Reyes, Acaimo
2004-05-01

Digital.CSIC (Spain)

151

Dpp Signaling Directs Cell Motility and Invasiveness during Epithelial Morphogenesis

Ninov, Nikolay; Menezes-Cabra, Sofia; Prat-Rojo, Carla; Manjón, Cristina; Weiss, A.; Pyrowolakis, George; Affolter, Markus; Martín-Blanco, Enrique
2010-03-23

Digital.CSIC (Spain)

153

Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections

Tenllado, Francisco; Martínez-García, Belén; Vargas, Marisol; Díaz-Ruíz, José Ramón
2003-03-20

Digital.CSIC (Spain)

157

A constitutive shade-avoidance mutant implicates TIR-NBS-LRR proteins in Arabidopsis photomorphogenic development

Faigón Soverna, Ana; Harmon, Franklin G.; Storani, Leonardo; Karayekov, Elizabeth; Staneloni, Roberto J.; Gassmann, Walter; Más, Paloma; Casal, Jorge J.; Kay, Steve A.; Yanovsky, Marcelo J.
2006-11-17

Digital.CSIC (Spain)

158

A conserved mechanism of Hedgehog gradient formation by lipid modifications

Guerrero, Isabel; Chiang, Chin

Article available at http://dx.doi.org/10.1016/j.tcb.2006.11.002 | Members of the Hedgehog (Hh) family of proteins are conserved morphogens that modulate cell fates in target tissues in different developmental systems. Dysregulation of Hh signaling results in a wide range of human diseases. The matu...

DRIVER (Spanish)