Sample records for CLONACION DNA (dna-cloning)

Sample records 1 - 4 shown.


Molecular cloning and expression in different microbes of the DNA encoding Pseudomonas putida U phenylacetyl-CoA ligase. Use of this gene to improve the rate of benzylpenicillin biosynthesis in Penicillium chrysogenum

Miñambres Rodríguez, Baltasar; Martínez Blanco, Honorina; Olivera, Elías R.; García, Belén; Díez, Bruno

8 pages, 5 figures, 3 tables.-- PMID: 8969218 [PubMed]. | The gene encoding phenylacetyl-CoA ligase (pcl), the first enzyme of the pathway involved in the aerobic catabolism of phenylacetic acid in Pseudomonas putida U, has been cloned, sequenced, and expressed in two different microbes. In both, th...

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Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon

Olivera, Elías R.; Miñambres Rodríguez, Baltasar; García, Belén; Muñiz, Carmen; Moreno, Miguel A.; Ferrández, Abel

6 pages, 7 figures, 1 table.-- PMID: 9600981 [PubMed] | Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the follo...

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Distribution, phylogeny, and growth of cold-adapted Picoprasinophytes in Arctic Seas

Lovejoy, Connie; Vicent, Warwick F.; Bonilla, Sylvia; Roy, Suzanne; Martineau, Marie-Josée; Potvin, Marianne; Massana, Ramon; Pedrós-Alió, Carlos

Digital.CSIC (Spain)


Cloning of the sole (Solea senegalensis) growth hormone-encoding cDNA

Pendón, Carlos; Martínez-Barberá, Juan P.; Pérez-Sánchez, Jaume; Rodríguez Martínez, Ramón B.; Grenett, Hernán

4 pages. | We report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly (A)+ RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an in...

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