Sample records for ADUCTOS DE DNA (dna adducts)
from WorldWideScience.org

Sample records 1 - 8 shown.



1

Aductos de hemoglobina y micronúcleos en trabajadores hospitalarios expuesto a óxido de etileno: Venezuela/ Hemoglobin adducts and micronuclei in hospital workers exposed to ethylene oxide: Venezuela

Rivero, Exila; Piñero, Sofia; González, Soraya; De Sousa, Luis; Rodríguez, Maritza; Barrio, Emilia; Delgado, Victor; Carrillo, Blanca; Guevara, Harold
2009-06-01

Resumen en español El óxido de etileno (OE) es un cancerígeno genotóxico con habilidad para reaccionar con macromoléculas biológicas, como la Hemoglobina y Acido desoxirribonucléico (ADN), formando N-(2-hidroxietil)valina (HEV) y 7-(2-hidroxietil)-guanina (7-HEG) respectivamente, compuestos llamados aductos. Estudio descriptivo, cuyo propósito fue evaluar trabajadores del área de esterilización de un hospital público expuestos a OE, mediante la determinación de aductos de hemoglo (mas) bina, micronúcleos en linfocitos (MN), tioeteres en orina y parámetros hematológicos. La muestra estuvo conformada por 10 individuos expuestos (GE) entre 24 y 56 años y 9 sujetos control (GC) con el mismo rango de edad de una universidad pública. El valor medio de N-(2-hidroxietil)valina (HEV) (pmol/g globina), en el GE fue 6758,5 ± 4143,6 y de 465,7 ± 484,5 en el GC (p Resumen en inglés Ethylene oxide (EO) is a genotoxic carcinogen, able to react with biological macromolecules such as hemoglobin and deoxyrebonucleic acid (DNA) to form N-(2-hydroxyethyl)valine (HEV) and 7-(2-hydroxyethyl)-guanine (7-HEG), respectively; these compounds are known as adducts. We evaluated biomarkers of effect and exposure workers from the central sterilization unit of a public hospital. The purpose of this descriptive study was to evaluate 10 EO-exposed employees (GE), rangi (mas) ng in age from 24 to 56 years, and 9 control workers from a public university (GC) of similar age.. The mean value of N-(2-hydroxyethyl)valine (HEV)(pmol/mol globin) in the GE group was 6758.6 ± 4143.6 and 465.7 ± 484.5 in the GC group; for micronuclei (MN) in lymphocytes (MNx1000 cells), the mean value in GE was 4.8 ± 2.5 and for GC it was 0.7 ± 0.8 in GC (p

Scientific Electronic Library Online (Spanish)

2

AFLATOXINAS: MECANISMOS DE TOXICIDAD EN LA ETIOLOGÍA DE CÁNCER HEPÁTICO CELULAR/ Aflatoxins and its mechanisms of toxicity in hepatic cancer

Urrego Novoa, José R; Díaz, Gonzalo J
2006-06-01

Resumen en español La aflatoxina B1 (AFB1), producida por algunos hongos del género Aspergillus, está entre los más potentes carcinógenos conocidos. Su acción carcinogénica se basa en la biotransformación por el sistema hepático microsomal P450 a AFB1-8,9-epóxido, un intermediario altamente reactivo capaz de unirse a las proteínas, a los ácidos ribonucleico y desoxirribonucleico; formando un compuesto estable con el N7 de los residuos guanil que puede causar mutaciones en el cod� (mas) �n 249 del gen p53 supresor de tumores. Esta alteración es característica de varios carcinomas, especialmente del carcinoma hepático en el hombre. La AFB1-8,9-epóxido forma uniones covalentes (aductos) con los residuos de guanina del ADN, que se excretan por vía urinaria y pueden utilizarse como biomarcadores de exposición en los grupos a riesgo de cáncer del hígado. Dado que las aflatoxinas son frecuentes en los granos de consumo humano, los países tropicales como Colombia, requieren estudios para determinar su presencia en alimentos de consumo humano y la de sus biomarcadores. Esta información podía ayudar a generar normatividades que permitan delinear políticas de salud pública para un adecuado control de estos carcinógenos. Resumen en inglés Aflatoxin B1 (AFB1) is produced by some fungus of the Aspergillus genus and it is one of the most potent known carcinogens. Action mechanisms, involves its biotransformation by the hepatic microsomal system P450 to AFB1-8, 9-epoxide. This epoxide is very reactive and electrophilic and may react with intracellular proteins, RNA or DNA. When the epoxide reacts with nucleic acids it forms an stable adduct with the N7 of the guanine residues and may induce mutations at codon (mas) 249 of the tumor suppressor gene p53. This alteration is characteristic of several carcinomas, particularly human liver carcinoma. The AFB1-8, 9-epoxide forms covalent bonds (adducts) with guanil residues in DNA; these adducts are excreted in the urine and can be used as biomarkers in risk groups for liver cancer. Given the high frequency of aflatoxins occurrence in grains for human consumption in tropical countries like Colombia, it is necessary to conduct studies to determine both the presence of aflatoxins in food and biomarkers in urine. This information could lead to the implementation of regulations intended to define public health measures for the control of aflatoxins exposure.

Scientific Electronic Library Online (Spanish)

3

Olive oil and cancer

López Martín, Sergio; Pacheco, Yolanda M.; Bermúdez, Beatriz; Abia, Rocío; García Muriana, Francisco J.
2004-03-30

Digital.CSIC (Spain)

5

Detection of DNA Adducts Derived from the Reactive Metabolite of Furan cis-2-Butene-1,4-dia

Byrns, Michael C.; Vu, Choua C.; Neidigh, Jonathan W.; Abad, José Luis; Jones, Roger A.; Peterson, Lisa A.

7 pages, 3 figures, 4 schemes. | Furan is a toxic and carcinogenic compound used in industry and commonly found in the environment. The mechanism of furan's carcinogenesis is not well-understood and may involve both genotoxic and nongenotoxic pathways. Furan undergoes oxidation by cytochrome P450 to...

DRIVER (Spanish)

6

Detection of DNA Adducts Derived from the Reactive Metabolite of Furan cis-2-Butene-1,4-dia

Byrns, Michael C.; Vu, Choua C.; Neidigh, Jonathan W.; Abad, José Luis; Jones, Roger A.; Peterson, Lisa A.
2006-02-28

Digital.CSIC (Spain)

7

Aromatic DNA adducts and polymorphisms in metabolic genes in healthy adults: findings from the EPIC-Spain cohort.

Agudo, Antonio; Peluso, Marco; Sala, Nuria; Capella, Gabriel; Munnia, Armelle; Piro, Sara; Marin, Fatima; Ibanez, Raquel

Aromatic compounds such as polycyclic aromatic hydrocarbons, arylamines and heterocyclic amines require metabolic activation to form metabolites able to bind to DNA, a process mediated by polymorphic enzymes. We measured aromatic DNA adducts in white blood cells by the (32)P-post-labelling assay in ...

DRIVER (Spanish)

8

Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides

González Pérez, Blanca; Lucas, María; Cooke, Leonie A.; Vyle, Joseph S.; Cruz, Fernando de la; Moncalián, Gabriel
2007-07-26

Digital.CSIC (Spain)