Sample records for CICLOHEXIMIDA (cycloheximide)
from WorldWideScience.org

Sample records 1 - 16 shown.



1

La testosterona inhibe las respuestas contráctiles del agonista adrenérgico α1, fenilefrina, asociadas con la liberación de calcio intracelular en la aorta de rata/ Testosterone inhibits the contractile responses to phenilephrine associated with the release of intracellular calcium in rat aorta

Castillo, Carlos; Castillo, Enrique F.; López, Jorge; López, Ruth M.
2006-02-01

Resumen en español Utilizando segmentos de aorta de rata sin endotelio inmersos en solución sin Ca2+, evaluamos la capacidad de la testosterona para modificar el efecto contráctil del agonista adrenérgico fenilefrina, así como el incremento en el tono de reposo (ITR) asociado con la entrada capacitativa de calcio por el sarcoplasma. La testosterona [10-5-10-4 M] inhibió significativamente la contracción activada por la fenilefrina [10-6 M] y el ITR. Estos efectos no fueron modificados (mas) con cicloheximida [10-5 M] (inhibidor de la síntesis protéica), flutamida [10-5 M] (antagonista de receptores androgénicos), o aminoglutetimida [10-5 M] (inhibidor de la citocromo P450 aromatasa). La testosterona también inhibió las respuestas contráctiles de la serotonina [10-5 M], pero no de la cafeína [10-2 M]. Además, la testosterona inhibió las contracciones del ácido ciclopiazónico [10-6 M] y de la ryanodina [10-5 M] asociadas con el ingreso capacitativo de Ca2+ mediante canales de Ca2+ tipo no L. Estos datos sugieren que la testosterona interfiere con la vía de transducción de los receptores acoplados a proteínas Gq-11, e inhibe la entrada capacitativa de Ca2+ a través de canales de Ca2+ tipo L y tipo no L; los efectos son no genómicos, independientes de receptores androgénicos, y de la conversión testosterona en estrógenos. Resumen en inglés Using endothelium-denuded rat aortic rings incubated in Ca2+-free solution, we assessed the ability of testosterone to influence the contractile effect of phenylephrine, and the increase in resting tone (IRT) associated with Ca2+ ability to cross the plasma membrane. The addition of testosterone[10-5-10-4M]5 min before phenylephrine [10-6 M], inhibited both phenylephrine-induced contraction and IRT. These changes were not affected by cycloheximide (10-5 M; a protein synth (mas) esis inhibitor of), flutamide (10-5 M; an androgenic receptor antagonist), or by adding aminoglutethimide (10-5 M; an aromatase inhibitor). Testosterone also blocked the contractile response to serotonin [10-5 M] but not to caffeine [10-2 M]. On the other hand, testosterone inhibited the contractile responses to cyclopiazonic acid (10-6 M; a selective Ca2+ -ATPase inhibitor) or ryanodine (10-5 M; an activator of sarcoplasmic reticulum Ca2+ -release channels) associated with capacitative Ca2+ influx through non-L-type Ca2+ channels. These data suggest that by acting on the cellular membrane, testosterone interferes with the signal transduction pathway of Gq-11 protein-coupled receptors, and inhibits capacitative Ca2+ influx through both L-type and non-L-type Ca²+ channels. These effects are non-genomic, non-mediated by the intracellular androgen receptor, and not due to the conversion of testosterone to estrogens.

Scientific Electronic Library Online (Spanish)

2

Triiodothyronine is required for the stimulation of type II 5'-deiodinase mRNA in rat brown adipocytes

Martínez de Mena, Raquel; Hernández, Arturo; Obregón, María Jesús
2002-05-01

Digital.CSIC (Spain)

4

Trehalose accumulation induced during the oxidative stress response is independent of TPS1 mRNA levels in Candida albicans

Zaragoza, Oscar; González-Párraga, Pilar; Pedreño, Yolanda; Alvarez-Peral, Francisco J.; Argüelles, Juan-Carlos
2003-06-06

Digital.CSIC (Spain)

6

Norepinephrine, tri-iodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mRNA during Brown adipocyte differentiation

Barroso, Isabel; Benito, Begoña; García-Jiménez, Custodia; Hernández, Arturo; Obregón, María Jesús; Santisteban, Pilar
1999-08-01

Digital.CSIC (Spain)

7

Mechanism of G2-Repair to preserve chromosome integrity and its inhibition by caffeine

López-Sáez, J. F.; González-Fernández, A.; Hernández, P.; Zamorano, E.; Navarrete, M. H.
1988-01-01

Digital.CSIC (Spain)

8

LPS-induced down-regulation of NO-sensitive guanylyl cyclase in astrocytes occurs by proteasomal degradation in nuclear bodies

Berciano, María T.; Baltrons, María Antonia; Pifarré, Paula; Lafarga, Miguel; García, Agustina
2007-07-25

Digital.CSIC (Spain)

9

Kahalalide F, a new marine-derived compound, induces oncosis in human prostate and breast cancer cells

Suárez, Yajaira; González, Laura; Cuadrado, Ana; Berciano, Maite; Lafarga, Miguel; Muñoz Terol, Alberto
2003-09-01

Digital.CSIC (Spain)

11

Identification of primary target genes of phytochrome signaling. Early transcriptional control during shade avoidance responses in Arabidopsis

Roig Villanova, Irma; Bou, Jordi; Sorin, Céline; Devlin, Paul F.; Martínez García, Jaime F.
2006-03-24

Digital.CSIC (Spain)

12

Glucocorticoid regulation of hepatic S-adenosylmethionine synthetase gene expression

Gil, Beatriz; Pajares, María A.; Mato, José M.; Álvarez, Luis
1997-03-01

Digital.CSIC (Spain)

15

Apoptosis in human thymocytes after treatment with glucocorticoids

Nieto, M. Ángela; González, A.; Gambón, F.; Díaz-Espada, F.; López-Rivas, Abelardo
1992-05-01

Digital.CSIC (Spain)

16

A novel p34cdc2-binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes

Ferby, Ingvar; Blazquez, Montserrat; Palmer, Amparo; Eritja Casadellà, Ramón; Nebreda, Angel R.
1999-08-15

Digital.CSIC (Spain)