Sample records for CELULAS CLONICAS (clone cells)
from WorldWideScience.org

Sample records 1 - 20 shown. Select sample records:



1

mRNA from NCB-20 cells encodes the N-methyl-D-aspartate/phencyclidine receptor: a Xenopus oocyte expression study

Lerma Gómez, Juan; Kushner, Leslie; Spray, David C.; Bennet, Michael V.; Zukin, R. Suzanne
1989-03-01

Digital.CSIC (Spain)

2

cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

Gutiérrez de Diego, Juana; Rodríguez García, David; Rodríguez Lorenzo, José Luis; Grappin, Philippe; Cervantes, Emilio
2006-03-01

Digital.CSIC (Spain)

3

Zea mays L. transglutaminase expression in Escherichia coli

Carvajal, Patricia; Villalobos, Enrique; Campos, Alexandre; Torné, J. Mª.; Barberà, Eduard; Santos, Mireya
2006-10-10

Digital.CSIC (Spain)

5

Transformation of Escherichia coli with DNA from Saccharomyces cerevisiae cell lysates

Adam, Ana Cristina; González Blasco, Gracia; Rubio Texeira, Marta; Polaina Molina, Julio
1999-12-01

Digital.CSIC (Spain)

7

The transcription factor early growth response factor-1 (EGR-1) promotes apoptosis of neuroblastoma cells

Pignatelli, Miguel; Luna Medina, Rosario de; Pérez-rendón, Arturo; Santos, Ángel; Pérez Castillo, Ana
2003-08-01

Digital.CSIC (Spain)

8

The brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferase

Vignols, Florence; Rigau, Joan; Torres, M. A.; Capellades, M.; Puigdomènech, Pere
1996-04-01

Digital.CSIC (Spain)

10

The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis

Abdu, Uri; González-Reyes, Acaimo; Ghabrial, Amin; Schüpbach, Trudi
2003-09-01

Digital.CSIC (Spain)

11

Temperature-dependent hypermutational phenotype in recA mutants of Thermus thermophilus HB27

Castán, Pablo; Casares, Lorena; Barbé, Jordi; Berenguer, José
2003-01-01

Digital.CSIC (Spain)

13

Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain

Becker, Nathalie; Seitanidou, Tania; Murphy, Paula; Mattéi, Marie-Geneviève; Topilko, Piotr; Nieto, M. Ángela; Wilkinson, David G.; Charnay, Patrick; Gilardi-Hebenstreit, Pascale
1994-07-01

Digital.CSIC (Spain)

15

Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoter

Butta, Nora; Larrucea, Susana; Alonso, S.; Rodríguez Martínez, Ramón B.; Arias-Salgado, Elena G.; Ayuso, Matilde S.; González-Manchón, Consuelo; Parrilla, Roberto
2006-05-09

Digital.CSIC (Spain)

16

Resistance of virus to extinction on bottleneck passages: Study of a decaying and fluctuating pattern of fitness loss

Lázaro, Ester; Escarmis Homs, Cristina; Pérez Mercader, Juan; Manrubia, Susanna C.; Domingo, Esteban
2003-09-01

Digital.CSIC (Spain)

18
19

Rapid selection in modified BHK-21 cells of a foot-and-mouth disease virus variant showing alterations in cell tropism

Escarmis Homs, Cristina; Carrillo, Elisa C.; Ferrer, Marcela; García, Juan F.; López, Nora; Tami, Cecilia; Verdaguer, Nuria; Domingo, Esteban; Franze-Fernández, Maria T.
1998-01-01

Digital.CSIC (Spain)

20

Pyruvate Carboxylase Is an Essential Protein in the Assembly of Yeast Peroxisomal Oligomeric Alcohol Oxidase

Ozimek, Paulina; van Dijk, Ralf; Latchev, Kantcho; Gancedo Rodríguez, Carlos; Wang, Dong Yuan; van der Klei, Ida J.; Veenhuis, Marten
2002-11-18

Digital.CSIC (Spain)

21

Protistan assemblages across the Indian Ocean, with a specific emphasis on the picoeukaryotes

Not, Fabrice; Latasa, Mikel; Scharek, Renate; Viprey, Manon; Karleskind, Pierre; Balagué, Vanessa; Ontoria-Oviedo, Imelda; Cumino, Andrea; Goetze, Erica; Vaulot, Daniel; Massana, Ramon
2008-06-28

Digital.CSIC (Spain)

22

Producción de anticuerpo Monoclonal (Acmo) con Especificidad Anti B, para ser utilizado como reactivo de Hemoclasificación/ Production of Monoclonal antibodies with Anti B specificity used As A blood typing reagent

León, Graciela; Cruz, Carlos; Rojas, Lola
2004-06-01

Resumen en español Resumen El objetivo de este trabajo fue producir un AcMo murino con especificidad anti B, el cual pudiera ser utilizado como reactivo de hemoclasificación. Ratones balb/c fueron inmunizados con sustancia antigénica de saliva de donante secretor, con el fin de obtener linfocitos esplénicos o esplenocitos productores de anticuerpos con especificidad anti B. Estos fueron fusionados con células de mieloma de ratón P3-X63.Ag8.653 utilizando PEG. Después de sembradas, las (mas) células híbridas o hibridomas resultantes que tuvieron la capacidad de secretar el anticuerpo requerido, fueron sometidas a clonación por dilución límite para asegurar su monoclonalidad. Posteriormente los anticuerpos fueron sometidos a evaluaciones inmunohematológicas e inmunoquímicas. Se obtuvo la clona BMS-4 secretora de anticuerpos IgM kappa, con especificidad anti B, los cuales demostraron tener una alta potencia, excelente avidez, gran sensibilidad y estabilidad en el tiempo. Dado el comportamiento demostrado por los anticuerpos en las evaluaciones realizadas, pensamos que esta clona puede ser utilizada para producir reactivo para la hemoclasificación. Resumen en inglés Abstract The purpose of this research was to produce murine monoclonal antibodies with anti B specificity to be used as reagents in red cells typing. Balb/c mice were immunized with substance B from saliva of a donor secreting B antigen, in order to obtain spleen cells or splenocytes producing antibodies with anti B specificity. These cells were fused with mouse myeloma cells P3-X63.Ag8.653 by using the PEG technique. After seeded, the hybridomas secreting the putative an (mas) tibodies were cloned through the limiting dilution technique to obtain monoclonality. Afterwards, the antibodies were evaluated using immunological and immunochemical methods. BMS-4 clone secreting antibodies IgM-kappa with anti B specificity were obtained, which displayed high potency, excellent avidity, great sensibility and long time stability. We think that this clone can be used to produce a red cell typing reagent.

Scientific Electronic Library Online (Spanish)

23

Polymorphism and structure of the gene coding for the alpha 1 subunit of the Artemia franciscana Na/K-ATPase

García-Sáez, Alberto; Perona Abellán, Rosario; Sastre Garzón, Leandro
1997-01-15

Digital.CSIC (Spain)

24

Peroxisomal Monodehydroascorbate Reductase. Genomic Clone Characterization and Functional Analysis under Environmental Stress Conditions

Leterrier, Marina ; Corpas, Francisco J. ; Barroso, Juan B. ; Sandalio, Luisa M. ; Río, Luis A. del

In plant cells, ascorbate is a major antioxidant that is involved in the ascorbate-glutathione cycle. Monodehydroascorbatereductase (MDAR) is the enzymatic component of this cycle involved in the regeneration of reduced ascorbate. Theidentification of the intron-exon organization and the promoter ...

DRIVER (Spanish)

25

Peroxisomal Monodehydroascorbate Reductase. Genomic Clone Characterization and Functional Analysis under Environmental Stress Conditions

Leterrier, Marina; Corpas, Francisco J.; Barroso, Juan B.; Sandalio, Luisa M.; Río, Luis A. del
2005-08-01

Digital.CSIC (Spain)

26

PAI-1 and functional blockade of SNAI1 in breast cancer cell migration

Fabre-Guillevin, Elizabeth; Malo, Michel; Cartier-Michaud, Amandine; Peinado, Héctor; Moreno-Bueno, Gema; Vallée, Benoît; Lawrence, Daniel A; Palacios, José; Cano García, Amparo; Barlovatz-Meimon, Georgia; Charrière-Bertrand, Cécile
2008-12-03

Digital.CSIC (Spain)

27

Obtención de heterohibridoma productor de anticuerpos monoclonales de tipo IgM contra el antígeno D del sistema Rh/ Obtention of a heterohybridoma for production of type IgM monoclonal antibodies against the D antigen of the Rh system

León-González, Graciela; Cruz, Carlos
2007-03-01

Resumen en español El objetivo de este trabajo fue obtener un heterohibridoma capaz de producir anticuerpo monoclonal con especificidad anti-D (Sistema Rh) de tipo IgM, que pueda ser utilizado como reactivo en la hemoclasificación. Las células mononucleares (CMN) se extrajeron de una muestra de sangre de mujer altamente sensibilizada, al 5to día del post parto de RN Rh positivo. Fueron sometidas a proceso de transformación con sobrenadante de cultivo (SNC) de células B95.8 rico en viri (mas) ones de Epstein Barr (VEB). Una vez transformadas y en crecimiento exponencial se fusionaron con células de la línea K6H6/B5, utilizando como agente fusógeno el PEG 4.000 en una proporción 1:1. Luego de la fusión se sembraron en placas de cultivo para posteriormente evaluar en los SNC de cada pozo, la formación de híbridos y la secreción de anticuerpos específicos. La eficiencia de fusión fue de 1,8 × 10-6, lográndose obtener una clona productora de anti-D IgM a la que denominamos BMS-9, la cual se pudo mantener en cultivo continuo por 3 meses produciendo anticuerpos a una concentración de 4 µg/mL en sobrenadante de cultivo (SNC). También se logró obtener anticuerpos en Sistema Capilar Artificial (SCA) alcanzando una concentración de 24 µg/mL. La potencia se determinó utilizando células Ror: En SNC a centrifugación inmediata (CI): 1 × 32, score 52; a 15´de incubación a TA: 1 × 1024 score 105; y a 37°C: 1 × 1024 score 105. Con el producto del SCA, a CI: 1 × 32 score 54; a 15´de incubación a TA: 1 × 8192 score 136; y a 37°C: 1 × 8192 score 136. Se detectó reactividad con eritrocitos D IIIa, D IV, D Va, D VI tipo IV, D VII, DFR, DNU, STEM+, DAR y DAU. No hubo reactividad con eritrocitos D IIIc, DI Va, D V tipo II, D VI tipo I, II y III, RoHAR, DOL y D débil tipo II. En estudio de campo, no se detectaron reacciones falsas negativas ni falsas positivas. Se obtuvo un heterohibridoma estable, productor de anti-D de tipo IgM, con cualidades suficientes para ser utilizado en la tipificación sanguínea. Dadas las excelentes cualidades del anticuerpo, estamos realizando evaluaciones con diferentes medios de dilución y con adición de anticuerpos tipo IgG, con el fin de fabricar un reactivo para uso en la hemoclasificación. Resumen en inglés The objective was to obtain a heterohybridoma capable of producing a monoclonal antibody with IgM type anti-D specificity (Rh system), that could be used as a reactive for hemoclasification. Mononuclear cells (MNC) were extracted from a blood sample of a highly sensitized woman, five days after giving birth to an Rh positive child. These were then transformed with the culture supernatant (CSN) of B05.8 cells, rich in Epstein Barr virus (EBV). Once transformed and in expon (mas) ential growth, they were fused with K6H6/B5 line cells using PEG 4.000 as a fusing agent in a 1:1 proportion. After fusion, they were seeded in culture plates in order to evaluate the formation of hybrids and the secretion of specific antibodies in the CSN of each well. The efficiency of the fusion was 1.8 × 10-6, making it possible to obtain an anti-D IgM producing clone, which we named BMS-9. This clone could be maintained in constant culture for three months, producing antibodies in a concentration of 4 µg/mL in de CSN. It was also possible to obtain antibodies with an Artificial Capilar System (ACS) reaching a concentration of 24 µg/mL. Potency was determined using Ror cells. In CSN at immediate centrifugation (IC): 1 × 32, score 52; 15´from incubation at room temperature (RT): 1 × 1,024 score 105. With that ACS product at IC: 1 × 32 score 54; 15´from incubation at RT: 1 × 8.192 score 136; and a 37°C: 1 × 8,192 score 136. Reactivity was detected with red cells D IIIa, D IV, D Va, D VI type IV, D VII, DFR, DNU, STEM+, DAR y DAU. There was no reactivity with red cells D IIIc, DI Va, D V type II, D VI types I, II y III, RoHAR, DOL and weak D type II. During field study, no false negative or false positive reactions were detected. A stable heterohybridoma was obtained, producer of IgM type anti-D, with enough qualities to be used in blood typing. Given the excellent qualities of the antibody, we are evaluating dilution media and the addition of type IgG antibodies in order to manufacture a reactive for use in hemoclassification.

Scientific Electronic Library Online (Spanish)

28

Novelty and spatio-temporal heterogeneity in the bacterial diversity of hypersaline Lake Tebenquiche (Salar de Atacama)

Demergasso, Cecilia; Escudero, Lorena; Casamayor, Emilio O.; Chong, Guillermo; Balagué, Vanessa; Pedrós-Alió, Carlos
2008-03-18

Digital.CSIC (Spain)

29

Monoclonal antibodies against sporangia and spores of Marteilla sp. (Protozoa: Ascetospora)

Robledo, José A. F.; Boulo, V.; Mialhe, E.; Desprès, B.; Figueras, Antonio
1994-01-01

Digital.CSIC (Spain)

30

Molecular cloning of Senegalese sole (Solea senegalensis) follicle-stimulating hormone and luteinizing hormone subunits and expression pattern during spermatogenesis

Cerdà, Joan; Chauvigné, François; Agulleiro, María J.; Marin, Elena; Halm, Silke; Martínez-Rodríguez, Gonzalo; Prat, Francisco
2008-05-01

Digital.CSIC (Spain)

31

Molecular cloning and pattern of expression of an alpha-L-fucosidase gene from pea seedlings

Augur, Christopher; Stiefel, Virginia; Darvin, Alan; Albersheim, Peter; Puigdomènech, Pere
1995-10-01

Digital.CSIC (Spain)

32

Molecular basis for a lack of correlation between viral fitness and cell killing capacity

Herrera, Mónica; García-Arriaza, Juan; Pariente, Nonia; Escarmis Homs, Cristina; Domingo, Esteban
2007-04-13

Digital.CSIC (Spain)

33
34

Metagenomic retrieval of a ribosomal DNA repeat array from an uncultured marine alveolate

Massana, Ramon; Karniol, Baruch; Pommier, Thomas; Bodaker, Idan; Béjà, Oded
2008-02-03

Digital.CSIC (Spain)

35

Lytic activity of the recombinant staphylococcal bacteriophage ΦH5 endolysin active against Staphylococcus aureus in milk

Obeso Díaz, José María; Martínez Fernández, Beatriz; Rodríguez González, Ana; García Suárez, María Pilar
2008-01-01

Digital.CSIC (Spain)

37

Linfoma no Hodgkin y aparato urinario: A propósito de un caso/ Non-Hodgkin´s lymphoma and urinary tract: About a case reported

Martín Laborda y Bergasa, F.; Lozano Lozano, D.; Gil Fernández, J.J.; Serrado Pardo, R.; Fernández Rañada, J.M.
2005-04-01

Resumen en español Describimos un caso de linfoma secundario no Hodgkin con presentación metacrónica en vejiga y riñón izquierdo, en un paciente con diferentes afectaciones extragonadales. Tanto en la vejiga como en el riñón se trataba de un linfoma de bajo grado tipo B de células centrofoliculares. Se sugiere la diseminación hematógena y afinidad del clon linfomatoso por el aparato urinario. Se establecen algunas consideraciones diagnósticas, así como sobre el enfoque terapéutico en este tipo de patologías. Resumen en inglés We pass to describe a case of non Hodgkin’s secondary lymphoma with metachronic affectation in bladder and left kidney, in a patient with different extranodals locations. Both in bladder and kidney, it was a low grade lymphoma type B with centrofolicular cells. We suggest haematogenous dissemination and affinity of lymphomatous clone to the urinary tract. We establish some diagnostic considerations, such about the therapeutic management in this type of pathologies.

Scientific Electronic Library Online (Spanish)

38

Laccases and their applications: A patent review

Kunamneni, Adinarayana; Plou Gasca, Francisco José; Ballesteros, Antonio; Alcalde Galeote, Miguel
2008-01-01

Digital.CSIC (Spain)

40

Isolation of the MIG1 gene from Candida albicans and effects of its disruption on catabolite repression

Zaragoza, Oscar; Rodríguez-Antona, Cristina; Gancedo Rodríguez, Carlos
2000-01-01

Digital.CSIC (Spain)

41

Isolation and characterization of casein kinase I from Dictyostelium discoideum

Moreno-Bueno, Gema; Calés Bourdet, Carmela; Behrens, M. Margarita; Fernández-Renart, Margarita
2000-07-15

Digital.CSIC (Spain)

43

Isolation and characterization of (gamma, delta) CD4+ T Cell clones derived from human fetal liver cells

Aparicio, Pedro; Alonso, José M.; Toribio, María Luisa; Marcos, Miguel A. R.; Pezzi, Luis; Martínez-Alonso, Carlos
1989-09-01

Digital.CSIC (Spain)

44

Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis

Martin-Martin, B.; Nabokina, S. M.; Blasi, J.; Lazo, Pedro A.; Mollinedo, Faustino
2000-10-01

Digital.CSIC (Spain)

45

Inventory and Functional Characterization of the HAK Potassium Transporters of Rice

Bañuelos, María A.; Garciadeblas, Blanca; Cubero, Beatriz; Rodríguez Ramirez, Alonso
2002-01-01

Digital.CSIC (Spain)

46

In vivo assembling of bacterial ribosomal protein L11 into yeast ribosomes makes the particles sensitive to the prokaryotic specific antibiotic thiostrepton

García-Marcos, Alberto; Morreale de León, Antonio; Guarinos, Esther; Briones, Elisa; Remacha, Miguel; Ortíz, Ángel R.; Ballesta, Juan P.G.
2007-10-16

Digital.CSIC (Spain)

47

In vivo assembling of bacterial ribosomal protein L11 into yeast ribosomes makes the particles sensitive to the prokaryotic specific antibiotic thiostrepton

García-Marcos, Alberto; Morreale de León, Antonio; Guarinos, Esther; Briones, Elisa; Remacha, Miguel; Ortíz, Ángel R.; Ballesta, Juan P.G.
2007-10-16

Digital.CSIC (Spain)

48

Immune networks : frequencies of antibody and idiotype producing B cell clones in various steady states

Bernabé, Rosa R.; Coutinho, Antonio; Martínez-Alonso, Carlos; Cazenave, Pierre-André
1981-08-01

Digital.CSIC (Spain)

53

High incidence of c-K-ras oncogenes in human colon cancer detected by the RNAse A mismatch cleavage method

Forrester, K.; Almoguera, Concepción; Jordano, Juan; Grizzle, W.E.; Perucho, M.
1987-01-01

Digital.CSIC (Spain)

54

Heterogeneidad clonal en epimastigotos de una cepa centroamericana de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae)/ CLONAL HETEROGENEITY OF A CENTRAL AMERICAN STRAIN OF Trypanosoma cruzi (KINETOPLASTIDA: TRYPANOSOMATIDAE)

CALDERÓN-ARGUEDAS, OLGER; TROYO, ADRIANA; VALERIO, IDALIA; CHINCHILLA, MISAEL
2002-01-01

Resumen en inglés The clonal heterogeneity properties of a Central American (San Salvador) strain of T. cruzi was evaluated using a macrophage culture system. Four clones named Clon 9, Clon 10, Clon 11 and Clon 12 were obtained by culture in a semisolid agar medium from the wild T. cruzi strain. Each clone and the parental strain were sub-cultured in Schneider medium with 10% fetal bovine serum before the infection process. The macrophages were cultured on cover slides and infected with ep (mas) imastigotes of each clone and the parental strain. The infection rate of the cells showed significant statistical differences (p

Scientific Electronic Library Online (Spanish)

55

Hemoglobinuria paroxística nocturna: Actualización/ Paroxysmal nocturnal haemoglobinuria

Milanés Roldán, María Teresa; Fernández Delgado, Norma; Fundora Sarraff, Teresa; Jaime Facundo, Juan Carlos; Hernández Ramírez, Porfirio
2003-04-01

Resumen en español La hemoglobinuria paroxística nocturna (HPN) es una enfermedad clonal y adquirida causada por una mutación somática en el gen PIG-A que se encuentra en el cromosoma X y codifica una proteina involucrada en la síntesis del glicosilfosfatidilinositol (GPI), el cual le sirve como anclaje a muchas proteínas de la membrana celular. La mutación ocurre en el stem cell hematopoyético y da lugar a una deficiencia parcial o total de la proteína PIG-A con la consecuente alte (mas) ración en la síntesis del GPI de anclaje; como resultado, una parte de las células sanguíneas serán deficientes de todas las proteínas ligadas al GPI. La ausencia de estas proteinas en la HPN explica algunos de los síntomas clínicos de la enfermedad, como la hemólisis intravascular mediada por el complemento, la trombosis venosa, el déficit de la hematopoyesis, etc; pero no el mecanismo mediante el cual el clon HPN se expande en la médula ósea. Varios estudios han demostrado que la inactivación del gen PIG- A por sí sola, no confiere una ventaja proliferativa al stem cell mutado, uno o más factores ambientales externos son necesarios para la expansión de este clon mutado, los cuales ejercen una presión selectiva a favor del clon HPN. La causa por el cual el clon HPN se estimula a proliferar podría ser un daño selectivo a la hematopoyesis normal. En el tratamiento de esta enfermedad se han utilizado varios agentes terapéuticos, pero el único tratamiento curativo es el trasplante de progenitores hematopoyéticos Resumen en inglés The paroxysmal nocturnal haemoglobinuria (PNH) is a clonal acquired disease caused by a somatic mutation in the PIG-A gene that is located in the chromosome X and codifies a protein involved in the synthesis of glycosil phosphatidylinositol (GPI), which serves as an anchor for many proetins of the cellular membrane. The mutations occurs in the hematopoietic stem cell and gives rise to a partial or total deficiency of the protein PIG-A with the subsequent alteration in the (mas) synthesis of the anchored GPI. As a result, a part of the blood cells will be lacking all the proteins bound to the GPI. The absence of these proteins in the NPH explains some of the clinical symptoms of the disease, such as the intravascular hemolysis mediated by the complement, the venous thrombosis, the deficit of hematopoiesis, etc., but not the mechanism by which the NPH clone expands into the bone marrow. Some studies have proved that the inactivation of the GPI-A gene does not confer a proliferative advantage to the mutated stem cell. One or more external environmental factors are needed for the expansion of this mutated clone. These factors exert a selective pressure in favor of the NPH clone. The cause for which the NPH clone is estimulated to proliferate may be a selective damage to the normal hematopoiesis. Several therapeutic agents have been used in the treatment of this disease, but the only curative treatment is the transplantation of hematopoietic progenitors

Scientific Electronic Library Online (Spanish)

56

Growth of uncultured heterotrophic flagellates in unamended seawater incubations

Massana, Ramon; Guillou, Laure; Terrado, Ramon; Forn, Irene; Pedrós-Alió, Carlos
2006-11-01

Digital.CSIC (Spain)

57

Genetic diversity and habitats of two enigmatic marine alveolate lineages

Groisillier, Agnès; Massana, Ramon; Valentin, Klaus; Vaulot, Daniel; Guillou, Laure
2006-03-29

Digital.CSIC (Spain)

58

Genetic dissection of a stem cell niche: the case of the Drosophila ovary

Bolívar, Jorge; Pearson, John; López-Onieva, Lourdes; González-Reyes, Acaimo
2006-11-01

Digital.CSIC (Spain)

59

Functional kainate-selective glutamate receptors in cultured hippocampal neurons

Lerma Gómez, Juan; Paternain, Ana V.; Naranjo, José Ramón; Mellström, Britt
1993-12-15

Digital.CSIC (Spain)

60

Forma de presentación de una amiloidosis primaria como polimialgia reumática y arteritis de células gigantes/ Primary systemic amyloidosis presenting as polymialgia rheumatica and giant cell arteritis

Pantoja Zarza, L.; Paniagua de la Riva, J.; Megido Lahera, M.; Ortiz de Saracho, J.
2001-04-01

Resumen en español La amiloidosis primaria o amiloidosis AL es una discrasia de células plasmáticas difícil de diferenciar del mieloma múltiple, que se caracteriza por el depósito de una proteína fibrilar de cadenas ligeras monoclonales en tejidos y órganos. Es una enfermedad poco frecuente, en la que un clon de células plasmáticas en la médula ósea produce inmunoglobulinas amiloidogénicas. En el 90% de los casos se detecta por inmunoelectroforesis inmunoglobulinas monoclonales (mas) en sangre u orina. Habitualmente se manifiesta como insuficiencia cardiaca, síndrome nefrótico o neuropatía periférica, pero excepcionalmente pueden existir síntomas sugestivos de arteritis de células gigantes (ACG) o polimialgia reumática (PMR). Aunque el número de clones de células plasmáticas en la médula ósea suele ser pequeño, la actitud terapeútica es la de una enfermedad proliferativa maligna, siendo la supervivencia media de 12 a 15 meses. Presentamos un paciente cuya primera manifestación de amiloidosis primaria fueron síntomas de ACG y PMR, y revisamos las principales características de esta enfermedad. Resumen en inglés Primary systemic amyloidosis or AL-amyloidosis is an uncommon disease characterized by the accumulation in vital organs of a fibrillar protein consisting of monoclonal light chains. It is a plasma-cell dyscrasia related to multiple myeloma where clonal plasma cells in the bone marrow produce immunoglobulins that are amyloidogenic. A monoclonal component is present in the serum or urine of 90% of patients. The presentation of most patients with AL amyloidosis is usually re (mas) lated to congestive heart failure, nephrotic syndrome o peripheral neuropathy, but there are unusual features suggesting giant cell arteritis (GCA) and polymyalgia rheumatic (PMR). Although in the majority of AL cases the plasma cells clone is small, the assumption is that the outcome of the disease is uniformly fatal (median survival 12-15 months) and treatment is analogous to those used in malignant proliferative disease. We describe a patient with AL amyloidosis who presented with manifestations of GCA and PMR, and we review the main characteristics of primary amyloidosis.

Scientific Electronic Library Online (Spanish)

61

Few mutations in the 5' leader region mediate fitness recovery of debilitated human immunodeficiency type 1 viruses

Yuste, Eloísa; Bordería, Antonio V.; Domingo, Esteban; López-Galíndez, Cecilio
2005-01-01

Digital.CSIC (Spain)

63

Expression of a maize cell wall hydroxyproline-rich glycoprotein gene in early leaf and root vascular differentiation

Stiefel, Virginia; Ruiz-Ávila, Luis; Raz, Regina; Vallés, María Pilar; Gómez, Jordi; Pagès, Montserrat; Martínez-Izquierdo, José Antonio; Ludevid, M. Dolors; Langdale, Jane A.; Nelson, Timothy; Puigdomènech, Pere
1990-08-01

Digital.CSIC (Spain)

67

Ensayo in vivo para determinar agentes extraños en células BHK-21 empleadas en la obtención de biológicos/ In vivo determination of adventitious agents in BHK-21 cells used in the production of biologicals

Pardo, Georgina; Almora, Ernesto; Fidalgo, Odalys; Zamora, Arelys; Rodríguez, Niurka; Pérez, Ela María
2000-06-01

Resumen en español La controversia sobre los parámetros de calidad de las células de línea como substrato para la obtención de productos farmacéuticos ha sido un tema de interés desde 1987. Las pruebas realizadas a las células, generalmente siguen las recomendaciones de los “Puntos a considerar en la Caracterización de Líneas Celulares empleadas para la Producción de Biológicos” entre las que se encuentra que estén libres de agentes adventicios. En este estudio al cl (mas) on 13 de células BHK-21 procedente de riñón de hámster sirio recién nacido, se le determina la presencia de agentes extraños in vivo; así como su inocuidad en animales de experimentación. La prueba in vivo incluyó la inoculación de embriones de pollo por cavidad alantoidea y saco vitelino, ratones lactantes y adultos, curieles y conejos. Todos los animales fueron observados para determinar signos clínicos de infección viral. En los embriones se observó la viabilidad. En todos los casos se mantuvo el 100% de los embriones y animales vivos y saludables durante el período de observación. Las células del banco estaban libres de virus hemaglutinantes y hemadsorbentes, virus de la coriomeningitis linfocítica y coxsackie. Los pesos de los animales inoculados fueron mayores que los de los animales controles. Las células no presentaron evidencia de agentes extraños en los ensayos in vivo y fueron bien toleradas e inocuas en los animales de experimentación. Resumen en inglés Acceptance of cell lines as a substrate for production of pharmaceuticals has been a controversial topic since 1987. The cells are generally tested according to the recommendations that appear in “Points to Consider in the Characterization of Cell Lines Used to Produce Biologicals”. They should be free from adventitious agents; among others in vitro and in vivo tests are recommended for this purpose. In this report BHK-21-cell clone 13 from new born Syrian ham (mas) ster kidney cells is assayed in vivo to determine adventitious agents; it is also tested for nonspecific innocuousness in experimental animals. In vivo tests included the inoculation of adult mice,suckling mice, guinea pigs, rabbits, and embryonated chicken eggs by the yolk sac and allantoic routes of inoculation. All animals were observed for clinical signs of viral infection. Egg viability was determined. The results showed that 100% of the animals and eggs remained healthy and survived the observation period. The cell bank was free of haemadsorbent, haemagglutinating, lymphocytic chroriomeningitis and Coxsakie viruses. The weights of the tested animals were higher than the weights of the control animals. The cells did not present evidences of adventitious agents in the in vivo assays and they were well accepted by the experimental animals.

Scientific Electronic Library Online (Spanish)

68

Eng1p, an Endo-1,3-β-Glucanase Localized at the Daughter Side of the Septum, Is Involved in Cell Separation in Saccharomyces cerevisiae

Martín-Cuadrado, Ana Belén; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R.; Baladrón, Victoriano; Ufano, Sandra; Dueñas, Encarnación
2002-10-01

Digital.CSIC (Spain)

70

Efficient virus extinction by combinations of a mutagen and antiviral inhibitors

Pariente, Nonia; Sierra, Saleta; Lowenstein, Pedro R.; Domingo, Esteban
2001-01-01

Digital.CSIC (Spain)

71

Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector

Fuerstenberg, S.; Beug, H.; Introna, M.; Khazaie, K.; Muñoz, A.; Ness, S.; Nordström, Kristina; Sap, J.; Stanley, I.; Zenke, M.; Vennström, Björn
1990-12-01

Digital.CSIC (Spain)

75

Distribution, phylogeny, and growth of cold-adapted Picoprasinophytes in Arctic Seas

Lovejoy, Connie; Vicent, Warwick F.; Bonilla, Sylvia; Roy, Suzanne; Martineau, Marie-Josée; Potvin, Marianne; Massana, Ramon; Pedrós-Alió, Carlos
2007-02-01

Digital.CSIC (Spain)

76

Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation

Jia, Gui-Quan; Gonzalo, José Ángel; Lloyd, Clare M.; Kremer, Leonor; Lu, Lei; Martínez-Alonso, Carlos; Wershill, B.K.; Gutiérrez Ramos, José Carlos
1996-11-01

Digital.CSIC (Spain)

79

Differential organ-specific response to salt stress and water deficit in nodulated bean (Phaseolus vulgaris)

Verdoy, D.; Lucas, M. Mercedes; Manrique, Esteban; Covarrubias, A. A.; Felipe, Mª Rosario de; Pueyo, José J.
2004-01-01

Digital.CSIC (Spain)

81

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from extremophilic microorganism Bacillus thermocatenulatus

Carrasco-López, César; Godoy, César; Rivas, Blanca de las; Fernández-Lorente, Gloria; Palomo, José Miguel; Guisán Seijas, José Manuel; Fernández-Lafuente, Roberto; Martínez-Ripoll, Martín; Hermoso, Juan A.
2008-01-01

Digital.CSIC (Spain)

82

Crash of a population of the marine heterotrophic flagellate Cafeteria roenbergensis by viral infection

Massana, Ramon; Campo, Javier del; Dinter, Christian; Sommaruga, Rubén
2007-07-06

Digital.CSIC (Spain)

84

Cloning, Expression, and Characterization of a Recombinant Gilthead Seabream Growth Hormone

Martínez-Barberá, Juan P.; Pendón, Carlos; Rodríguez Martínez, Ramón B.; Pérez-Sánchez, Jaume; Valdivia, Manuel M.
1994-11-01

Digital.CSIC (Spain)

85

Class III antiarrhythmic effects of zatebradine. Time-, state-, use-, and voltage-dependent block of hKv1.5 channels

Valenzuela, Carmen; Delpón, Eva; Franqueza, Laura; Gay, Pilar; Pérez, Onésima; Tamargo, Juan; Snyders, Dirk J.
1996-08-01

Digital.CSIC (Spain)

86

Characterization of two divergent endo-beta-1,4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit

Llop-Tous, Immaculada; Domínguez-Puigjaner, Eva; Palomer Tarrides, Xavier; Vendrell Melich, Miguel
1999-04-01

Digital.CSIC (Spain)

88

Characterization of the CaENG1 gene encoding an endo-1,3-β-glucanase involved in cell separation in Candida albicans

Esteban, Pedro Felipe; Ríos, Inmaculada; García, Raúl; Dueñas, Encarnación; Plá, Jesús; Sánchez-Pérez, Miguel; Vázquez de Aldana, Carlos R.; Rey Iglesias, Francisco del
2005-11-16

Digital.CSIC (Spain)

89

Characterization of Genomic Clones and Expression Analysis of the Three Types of Superoxide Dismutases During Nodule Development in Lotus japonicus

Rubio Luna, Mari Carmen; Becana Ausejo, Manuel; Sato, Shusei; James, Euan K.; Tabata, Satoshi; Spaink, Herman P.
2007-03-01

Digital.CSIC (Spain)

92

Analysis of the pmsCEAB Gene Cluster Involved in Biosynthesis of Salicylic Acid and the Siderophore Pseudomonine in the Biocontrol Strain Pseudomonas fluorescens WCS374

Mercado-Blanco, Jesús; Van der Drift, Koen M. G. M.; Olsson, Per E.; Thomas-Oates, Jane E.; van Loon, Leendert C.; Bakker, Peter A. H. M.
2001-03-01

Digital.CSIC (Spain)

93

Analysis of microbial community during biofilm development in an anaerobic wastewater treatment reactor

Fernández, Nuria; Díaz, Emiliano Enrique; Amils, Ricardo; Sanz, José L.
2008-07-01

Digital.CSIC (Spain)

94

ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3

Horiuchi, Horiuchi; Silverman, Neal; Piña, Benjamín; Marcus, Gregory A.; Guarente, Leonard
1997-06-01

Digital.CSIC (Spain)

95

A novel p34cdc2-binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes

Ferby, Ingvar; Blazquez, Montserrat; Palmer, Amparo; Eritja Casadellà, Ramón; Nebreda, Angel R.
1999-08-15

Digital.CSIC (Spain)

96

A novel extracellular matrix protein from tomato associated with lignified secondary cell walls

Domingo, Concepción; Gómez, María Dolores; Cañas, Luis; Hernández-Yago, José; Conejero, Vicente; Vera, Pablo
1994-01-01

Digital.CSIC (Spain)

97

A comparative kinetic analysis of the reactivity of plant, horse, and human respiratory cytochrome c towards cytochrome c oxidase

Rodríguez-Roldán, Vicente; García-Heredia, José M.; Navarro, José A.; Hervás, Manuel; Cerda, Berta de la; Molina-Heredia, Fernando P.; Rosa, Miguel A. de la
2006-06-12

Digital.CSIC (Spain)

98

A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases

Domínguez-Puigjaner, Eva; Llop, Inmaculada; Vendrell Melich, Miguel; Prat, Salomé
1997-07-01

Digital.CSIC (Spain)