Sample records for REACCIONES EN CADENA (chain reactions)
from WorldWideScience.org

Sample records 1 - 20 shown. Select sample records:



1

Reacciones transfusionales en el Hospital Docente Provincial “Comandante Faustino Pérez” de la provincia de Matanzas/ Transfusion reactions at “Comandante Faustino Pèrez” Provincial University Hospital in the province of Matanzas

Silva Ballester, Hilda M; Rojas Zúñiga, Mercedes; González Lorenzo, Ariadna; Ballester Santovenia, José M; Bencomo Hernández, Antonio; Alfonso Valdés, María Elena; Blanco García, Antonio
2007-08-01

Resumen en español A partir del año 2003, en el Hospital Docente Provincial “Comandante Faustino Pérez” se inició el control y análisis de los efectos adversos de la transfusión de sangre, mediante el reporte y estudio de todas las reacciones transfusionales, para conocer su frecuencia, tipo y gravedad, así como su relación con el componente utilizado, los días de extraído y el sexo del receptor. Se reportaron los errores detectados y subsanados antes del proceder. Este (mas) estudio se realizó bajo la asesoría y control del Banco de Sangre Provincial “Minerva Duarte”, como parte de la vigilancia sobre los productos sanguíneos y la cadena transfusional, con el objetivo de disminuir los efectos adversos e incrementar la seguridad transfusional. Los datos obtenidos demuestran disminución del número de reacciones adversas, de 52 en el 2002 a 14 en el 2005. Las más frecuentes fueron la febril no hemolítica y las alérgicas; la más grave resultó la contaminación bacteriana. Los errores más frecuentes subsanados antes de la transfusión fueron por equivocación del grupo en la bolsa a transfundir. El componente que más reacciones originó fue el concentrado de hematíes y el sexo femenino fue el que más reacciones presentó. El estudio permitió la adopción de medidas correctivas y preventivas para disminuir las reacciones e incrementar la seguridad transfusional en el centro hospitalario. Resumen en inglés From 2003 on, it was started the control and analysis of the adverse effects of blood transfusion at “Comandante Faustino Pérez” Provincial University Hospital by the report and study of all the transfusion reactions in order to know their frequency, type and severity, as well as their relation to the component used, the date of the extraction, and the sex of the recipient. The errors detected and corrected before the procedure were reported. This study was c (mas) onducted under the counselling and control of “Minerva Duarte” Provincial Blood Bank as part of the vigilance of the blood products and the transfusion chain aimed at reducing the adverse effects and at increasing transfusion safety. The data obtained showed a decrease of the number of adverse reactions from 52, in 2002, to 14, in 2005. The most common reactions were the non-hemolytic febrile reactions and the allergic reactions, whereas the most serious was bacterial contamination. The most frequent amended mistakes before transfusion were those made by the group with the transfusion bag. The packed cells proved to be the component that originated the greatest amount of reactions. Females had more reactions. The study allowed to take corrective and preventive measures to lower the reactions and improve the transfusion safety in the hospital.

Scientific Electronic Library Online (Spanish)

2

Detección de Leishmania (Viannia) Braziliensis en el Endotelio Vascular de Lesiones de Pacientes con Leishmaniasis Cutánea Localizada

Lugo-Yarbuh, Ana; Valera, Manuel; Alarcón, Maritza; Moreno, Elio; Premoli-Percoco, Gloria; Colasante, Cesare
2003-03-01

Resumen en español En el presente estudio se analizaron las lesiones dérmicas de 8 pacientes con leishmaniasis cutánea (LC), procedentes de caseríos ubicados en las cercanías de las cuencas de los ríos Chama-Mocotíes del estado Mérida, Venezuela. Las lesiones de los pacientes fueron diagnosticadas de acuerdo al criterio clínico, parasitológico e inmunocitoquímico. Los parásitos de las lesiones cutáneas fueron identificados por la reacción en cadena de la polimerasa (PCR) como L (mas) eishmania (Viannia) braziliensis. Histopatológicamente, las lesiones mostraron infiltrado inflamatorio de células mononucleares y polimorfonucleares. En la dermis se observaron reacciones granulomatosas y amastigotos. Los cortes histológicos de una de las lesiones del paciente Nº 5 mostraron vasos sanguíneos morfológicamente afectados con antígeno de Leishmania en la superficie endotelial, vasos dilatados con parásitos en su luz y células mononucleares. El frotis de la misma lesión cutánea mostró amastigotos en el citoplasma de neutrófilos. Las reacciones de inmunofluorescencia indirecta (IFI) e inmunoperoxidasa indirecta (PAP) realizadas sobre estas lesiones, revelaron amastigotos y material antigénico adsorbido sobre las paredes de los vasos sanguíneos, así como amastigotos libres en la luz de los vasos sanguíneos dérmicos. Basados en estos resultados, se concluye que en la piel de algunas lesiones cutáneas, es posible reconocer la presencia de los parásitos tanto intracelulares como extracelulares, en estrecho contacto con los vasos sanguíneos dérmicos y libres en la luz capilar, lo que favorecería el desplazamiento del parásito por vía sanguínea. Este proceso determinaría la posibilidad de producir infecciones secundarias y la propagación de la infección en las áreas endémicas de leishmaniasis, donde el parásito circula entre los reservorios domésticos y silvestres, los insectos vectores y el hombre que viven en esas áreas. Resumen en inglés In the present study, we examined the cutaneous lesions of 8 patients with cutaneous leishmaniasis, from regions situated near the rivers Chama-Mocoties, in Merida state, Venezuela. The lesions of the patients were diagnosed on the basis of clinical, parasitological and inmmunological examinations. The Polymerase Chain Reaction (PCR) assay showed infection by Leishmania (Viannia) braziliensis in cutaneous lesions samples. Histopathology of skin biopsy specimens showed inf (mas) lammatory infiltrates of mononuclear and polymorphonuclear leukocytes. Granulomatous reactions and amastigotes were observed in the dermis. The histological sections of the cutaneous lesions in one patient (Nº 5), showed alterations in the integrity of dermal blood vessels, leishmanial antigens on the superficial endothelium and free parasites in the capillary lumen and inside mononuclear cells. The Leishmania amastigotes also were detected in the cytoplasm of neutrophils in Giemsa-stained imprints. The skin biopsies examined using immunofluorescence (IFI) and immunoperoxidase assay (PAP), showed amastigotes and antigenic material adsorbed on the vecinity of the walls of dermal microvessels. Based on these results, we concluded that in the cutaneous lesions it is possible to show the presence of intra and extracellular parasites, attached to wall of the dermal blood vessels and free, in the capillary lumen. The circulating parasites in peripheral blood may allow the possibility of developing secondary infections as well as the propagation of the infection in endemic areas of leishmaniasis, where the parasite circulates between domestic and wild reservoirs, vector insects and humans living in those areas.

Scientific Electronic Library Online (Spanish)

3

Detección molecular de patógenos emergentes de importancia médica y veterinaria en garrapatas capturadas sobre caballos domésticos/ Molecular detection of emerging pathogens of medical and veterinary importance in ticks found in domestic horses

Rodríguez, Islay; Gern, Lise; Rais, Olivier; Fuentes, Omar; González, Roberto; Fernández, Carmen
2009-04-01

Resumen en español INTRODUCCIÓN: en la actualidad son varias las especies de patógenos emergentes de importancia médica y veterinaria transmitidos por garrapatas. Los estudios sobre estos agentes y sus enfermedades han sido escasos en Cuba. OBJETIVOS: conocer la presencia de algunos de estos patógenos en garrapatas cubanas que afectan el ganado equino. MÉTODOS: se procesaron 95 garrapatas colectadas de caballos domésticos, conservadas en alcohol e identificadas taxonómicamente según (mas) claves convencionales. A cada una se le realizó extracción de ADN y posteriormente diferentes reacciones en cadena de la polimerasa utilizando cebadores específicos para los grupos microbianos Borrelia burgdorferi sensu lato, Anaplasma-Ehrlichia, y Babesia-Theileria. Cada uno de los productos de las reacciones en cadena de la polimerasa fue sometido a hibridaciones en línea reversa utilizando sondas para cada grupo en cuestión, así como específicas para las principales especies de estos. RESULTADOS: las garrapatas estudiadas pertenecían a las especies Dermacentor (Anocentor) nitens (60 %), Amblyomma cajennense (38 %) y Rhipicephalus (Boophilus) microplus (2 %). Se detectaron 7 garrapatas Dermacentor (Anocentor) nitens infectadas con bacterias del grupo Anaplasma/Ehrlichia, y no se pudo identificar la especie en cuestión con las sondas utilizadas. Una de estas garrapatas estaba además coinfectada con Babesia bovis. CONCLUSIONES: se sugiere la circulación de una nueva especie de Anaplasma o Ehrlichia no reportada antes en Cuba, por lo que se necesita estudiar un número mayor de garrapatas, así como la incorporación de nuevas sondas en la hibridación en línea reversa u otras metodologías que permitan conocer con exactitud las especies que pudiesen afectar hoy día los caballos domésticos. Resumen en inglés INTRODUCTION: at present, there are several tick-borne emerging pathogen species of medical and veterinary importance. Few studies on these agents and its diseases have been made in Cuba. OBJECTIVES: to determine the presence of some of these pathogens in Cuban ticks existing in the equine cattle. METHODS: ninety five ticks collected from domestic use horses were processed, preserved in alcohol and taxonomically identified according to the set classifications. Their DNA w (mas) as extracted and subjected to several polymerase chain reactions with specific primers for microbial groups Borrelia burgdorferi sensu lato, Anaplasma-Ehrlichia, y Babesia-Theileria. Each of the products from polymerase chain reactions underwent reverse line blot hybridation using probes for each group as well as specific probes for the main species included in these groups. RESULTS: the studied ticks belonged to Dermacentor (Anocentor) nitens (60 %), Amblyomma cajennense (38 %) y Rhipicephalus (Boophilus) microplus (2 %). Seven Dermacentor (Anocentor) nitens ticks infested with Anaplasma/Ehrlichia bacteria were detected but the species in question could not be detected by the used probes. One of these ticks was also co-infested with Babesia bovis. CONCLUSIONS: it is suggested that a new species of Anaplasma o Ehrlichia, not reported in Cuba before now, is circulating, so studying a higher number of ticks is needed and new probes in reverse line blot hybridation or other methodologies must be incorporated to allow exactly determining the species that may affect the Cuban domestic horses at present.

Scientific Electronic Library Online (Spanish)

4

Mecanismos celulares y moleculares de la enfermedad cerebrovascular isquémica/ Cellular and molecular mechanisms of ischemic cerebrovascular disease

Guevara García, Mariela; Rodríguez, Raisa; Álvarez León, Alina; Riaño Montalvo, Annia; Rodríguez Rodríguez, Pedro Camilo
2004-08-01

Resumen en español Se realizó una revisión bibliográfica acerca de los mecanismos celulares y moleculares que se producen en la enfermedad cerebrovascular isquémica. El fallo en la producción energética, acidosis láctica, aumento del calcio citosólico, exceso de radicales libres y acúmulo extracelular de neurotransmisores, con la consecuente activación de receptores y estimulación neuronal en circunstancias de fallo de aporte de oxígeno y glucosa, parecen ser pasos importantes e (mas) n los procesos que conducen a la muerte neuronal. Estos mecanismos conducirían a un daño secundario de la microcirculación cerebral, por edema y lesión endotelial, formación de agregados celulares intravasculares y alteraciones de la permeabilidad y reactividad vascular, lo que ocasionaría el fenómeno de "no reflujo", se cerraría el círculo y se perpetuaría el proceso. Esta cascada de reacciones químicas en las células nerviosas y el conocimiento de estas alteraciones del metabolismo celular es de sumo interés puesto que permite investigar fármacos que bloqueando esta cadena de reacciones lleven a una mejor recuperación funcional. Resumen en inglés A bibliographic review was made on the cellular and molecular mechanisms that are produced in the ischemic cerebrovascular disease. The failure in the energy production, lactic acidosis, increase of cytosolic calcium, excess of free radicals and extracellular crowd of neurotransmissors, with the subsequent activation of receptors and neuronal stimulation under circumstances of poor glucose and oxygen supply seem to be important steps in the processes leading to neuronal d (mas) eath.These mechanisms would lead to a secondary damage of cerebral microcirculation due to edema and endothelial lesion, formation of intravascular cellular aggregates and alterations of permeability and vascular reactivity that would cause the "non reflux" phenomenon, the circle would close and the process would be perpetuated. This series of chemical reactions in the nervous cells and the knowledge of these alterations of cellular metabolism are very interesting, since they allow to investigate drugs that by blocking this chain of reactions lead to a better functional recovery.

Scientific Electronic Library Online (Spanish)

5

Reporte del primer caso de enfermedad de Chagas transplacentaria analizado por AP-PCR en Moniquirá, Boyacá/ The first case of congenital Chagas' disease analyzed by AP-PCR in Colombia

Pavia, Paula Ximena; Montilla, Marleny; Flórez, Carolina; Herrera, Giomar; Ospina, Juan Manuel; Manrique, Fred; Nicholls, Rubén Santiago; Puerta, Concepción
2009-12-01

Resumen en español Introducción. La principal vía de transmisión de la enfermedad de Chagas es por medio de los insectos vectores de la familia Reduviidae. Sin embargo, el parásito también puede ser transmitido de madres infectadas al feto in utero. Hasta la fecha no existen informes de casos de Chagas transplacentario en Colombia. Objetivo. Presentar un caso de transmisión transplacentaria ocurrido en Moniquirá, Boyacá, Colombia, y confirmarlo con el análisis de las cepas aisladas (mas) de la madre y de su bebé mediante reacción en cadena de la polimerasa (PCR) con cebadores arbitrarios. Materiales y métodos. De los hemocultivos positivos de una madre chagásica y su hijo, se extrajo el ADN de los tripanosomas y se caracterizá la especie y grupo por PCR. El genotipo de las cepas se determinó mediante AP-PCR con los iniciadores basados en los genes de b-globina (5'™-CCTCACCTTCTTTCATGGAG-3'™) y del ARNr 16S (5'™-ACGGGCAGTGTGTACAAGACC-3'™), en reacciones diferentes. Resultados. Las cepas de Trypanosoma cruzi aisladas de los hemocultivos de la madre y de su hijo mostraron el mismo perfil de amplificación por ambas pruebas de AP-PCR, concordante con el observado en las cepas T. cruzi I utilizadas como control. En los hemocultivos procedentes del reción nacido se encontrá también T. cruzi II. Conclusiones. Éste es el primer caso de enfermedad de Chagas transplacentaria reportado en el municipio de Moniquirá, que demuestra que esta forma de transmisión ocurre en el país. La presencia de infección mixta por ambos grupos de T. cruzi en las muestras del recién nacido, sugiere infección mixta en la madre, con mayor prevalencia de T. cruzi I, al menos en el hemocultivo. Resumen en inglés Introduction. The main route of Chagas disease transmission is through vectors of the insect family Reduviidae. However, the parasite can also be transmitted from infected mothers to their fetus in utero. Until now, no cases of congenital Chagas disease have been reported in Colombia. Objective. A congenital Chagas disease case occurred in Moniquirá County, Boyacá, Colombia. It was confirmed by comparing strains isolated from the mother and her baby using polymerase cha (mas) in reaction (PCR) with arbitrary primers. Materials and methods. The parasite DNA was extracted from positive blood cultures of the aflicted mother and her son. The species confirmation and group detection were performed by PCR. The strain genotypes were determined by AP-PCR with two oligonucleotides based on the genes for the b-globin (5'™-CCTCACCTTCTTTCATGGAG-3'™) and 16S RrNA (5'™-ACGGGCAGTGTGTACAAGACC-3'™), in differente reactions. Results. The T. cruzi strains isolated from the blood cultures of the mother and her son showed the same amplification profile by the two AP-PCR tests; this corresponded with profiles of the T. cruzi I strains used as controls. However, T. cruzi II was also found in the blood culture from the newborn. Conclusions. This is the first case of Chagas disease transmission reported in Moniquirá, demonstrating that this form of transmission occurs in Colombia. The presence of both groups of T. cruzi in the newborn sample suggests mixed infection in the mother as well, with a higher prevalence of T. cruzi I, at least in the mother's blood culture.

Scientific Electronic Library Online (Spanish)

6

Simulación de la Polimerización por Radicales Libres en el Intervalo Completo de Conversión/ Simulation of Free Radical Polymerization in the Entire Conversion Range

Rivero, Pastor
2006-01-01

Resumen en español En este trabajo se presenta la simulación de la polimerización por radicales libres de metacrilato de metilo para obtener la conversión y los pesos moleculares promedio, considerando el control por difusión en las reacciones de iniciación, propagación, transferencia y terminación. La parte central del trabajo consiste en la aplicación de un método propuesto por los autores para la solución aproximada de las ecuaciones cinéticas, cuando el coeficiente de velocid (mas) ad de terminación depende del tamaño de los radicales. La estrategia del método consiste en reconstruir la distribución de longitudes de cadena de la población de radicales, a partir de sus momentos, con el fin de obtener los momentos de la distribución de velocidades de terminación. Los resultados obtenidos de conversión y pesos moleculares promedio muestran una buena concordancia con los datos experimentales reportados Resumen en inglés In this study the simulation of methyl methacrylate free radical polymerization is presented, in order to obtain the conversion and the average molecular weights, considering diffusion-controlled initiation, propagation, chain transfer and termination reactions. The central part of the work is the application of a method previously proposed by the authors for the approximate solution of the kinetic equations when the termination rate coefficient depends on the chain lengt (mas) h of the radicals The strategy of the method is to reconstruct the chain length distribution of radicals from its moments to obtain the termination rate distribution moments. The calculated conversion and average molecular weights are in good agreement with previously reported experimental data

Scientific Electronic Library Online (Spanish)

7

Bajo peso al nacer, desarrollo puberal y presión arterial en adolescentes del sexo femenino/ Low weight at birth, pubertal development and blood pressure in female adolescents

Gorte Quiñones, Annia Dolores; Bacallao Gallestey, Jorge; Cabrera Hernández, Mayte; Rosales del Pino, Alicia Maria; Otero Martínez, Alfredo
2008-12-01

Resumen en español Se estudió la presión arterial en una muestra de adolescentes del sexo femenino, sanas, con edades comprendidas entre doce y catorce años. Se determinó la condición de las muchachas con respecto a la menarquia. El objetivo de la investigación fue confirmar la conocida asociación inversa entre el peso al nacimiento y la presión arterial, estudiar la asociación entre el peso al nacimiento y la edad de la menarquia y poner a prueba una hipótesis sobre el tamaño re (mas) lativo del riñón con respecto al volumen corporal y su asociación con las cifras de presión arterial. Se llevó a cabo un estudio descriptivo, observacional y transversal a dicha muestra. Los resultados evidenciaron que las adolescentes que tienen menarquia más temprana tienen cifras más altas de tensión arterial y mayor índice de masa corporal. Un indicador alométrico de la relación entre el volumen renal y el índice de masa corporal también se encuentra disminuido en este grupo de muchachas. En las adolescentes que nacieron con peso insuficiente, la menarquia ocurre, en promedio, casi un año antes que en las que nacieron con peso normal. Se concluye que el peso insuficiente al nacimiento favorece, bajo ciertas condiciones, un adelanto en los eventos puberales, lo cual se acompaña de reacciones adaptativas en el organismo, se determinó que existe correlación significativa entre estas variables con una p=0.014. Esta cadena de sucesos contribuye a un incremento en las cifras de prensión arterial. Resumen en inglés Blood pressure was studied in a sample of 12-14-year-old healthy female adolescents, determining the condition of their menarche. The aim of the present research is to prove the known inverse association between the body weight at birth and blood pressure, to study the association between the body weight at birth and the age of the menarche and to prove a hypothesis on the relative size of the kidney with respect to the body volume and its association with the counts of t (mas) he blood pressure. A descriptive observational and cross sectional study was performed in the sample. Results showed that adolescents with earlier menarche have higher counts of blood pressure as well as a greater body mass index. An alometric index in the relationship between the renal volume and the body mass index is also decreased in this group of adolescents. In those born with a low body weight, menarche appears almost a year before than in those born with a normal body weight. It is concluded that the low body weight at birth is in favour of , under some circumstances, an increase of the pubertal events, being accompanied by adaptative reactions in the economy, it was also determined that there is a significant correlation among these variables (p =0.014). This chain of events leads to an increase of blood pressure counts.

Scientific Electronic Library Online (Spanish)

8

Virus herpes humano 8: Detección en células mononucleares de sangre periférica de pacientes con la infección por el virus de inmunodeficiencia humana con/sin sarcoma de Kaposi

Hernández, Dimas E; Riera, Jorge; Pinto, José; Marín, María E; López, José L
2008-03-01

Resumen en español El ácido nucleico proveniente del virus herpes humano 8 está presente en las células mononucleares de sangre periférica de un 50 % a 90 % de los pacientes con sarcoma de Kaposi, y 7 % a 10 % de los pacientes con la infección por el virus de inmunodeficiencia humana sin sarcoma de Kaposi. Nosotros estudiamos la prevalencia del virus herpes humano 8 en células mononucleares de sangre periférica provenientes de pacientes con la infección por el virus de inmunodeficie (mas) ncia humana con/ sin sarcoma de Kaposi. Setenta y seis pacientes con la infección por el virus de inmunodeficiencia humana sin sarcoma de Kaposi y 15 pacientes con sarcoma de Kaposi asociado a la infección por el virus de inmunodeficiencia humana se incluyeron en el estudio. El ácido desoxirribonucleico se extrajo utilizando el método de fenol/cloroformo. El ácido desoxirribonucleico fue amplificado a través de la reacción en cadena de la polimerasa utilizando las sondas KS1 y KS2 específicas para el ORF 26 del virus herpes humano 8. Las reacciones se consideraron positivas sólo si los productos de la reacción en cadena de la polimerasa hibridizaron en la región esperada de 233 pares de bases. Ninguno de los pacientes con la infección por el virus de inmunodeficiencia humana mostró la presencia del virus herpes humano 8 en las células mononucleares de sangre periférica, y después de un seguimiento de 2 años, ninguno ha desarrollado sarcoma de Kaposi. El virus herpes humano 8 se detectó en las células mononucleares de sangre periférica del 20 % de los pacientes con sarcoma de Kaposi Todos los pacientes pertenecían al grupo de “alto riesgo”, y eran varones homosexuales. Ninguno recibió transfusiones sanguíneas. Estos datos preliminares sugieren que la prevalencia del virus herpes humano 8 en las células mononucleares de sangre periférica de los pacientes con la infección por el virus de inmunodeficiencia humana con/sin sarcoma de Kaposi es probablemente baja en comparación con pacientes provenientes de Estados Unidos y Europa. Resumen en inglés The nucleic acid of human herpes virus 8 is present in the peripheral blood mononuclear cells of between 50 % and 90 % of Kaposi sarcoma patients, 7 % and 10 % of human immunodeficiency virus infected patients without Kaposi sarcoma. We studied the prevalence of human herpes virus 8 in peripheral blood mononuclear cells from patients with human immunodeficiency virus infection with/without Kaposi sarcoma. Seventy-six patients with human immunodeficiency virus infection wi (mas) thout Kaposi sarcoma and 15 patients with Kaposi sarcoma associated with human immunodeficiency virus infection were included. Desoxirribonucleico acid was extracted from the samples by a standard phenol/chloroform extraction procedure. Desoxirribonucleico acid was polimerase chain reaction amplified using Kaposi sarcoma 1 and Kaposi sarcoma 2 primers specific for the human herpes virus 8 ORF26. Polimerase chain reaction reactions were considered positive only if the polimerase chain reaction products hybridized in the expected 233 bp region. None of human immunodeficiency virus infected patients showed the presence of human herpes virus 8 in the peripheral blood mononuclear cells, and after a follow-up of 2 years, none has developed Kaposi sarcoma. Human herpes virus 8 was detected in the peripheral blood mononuclear cells from 20 % of patients with Kaposi sarcoma. All patients belonged to a “high risk group”, were male and homosexuals. None received blood transfusion. These preliminary data suggest thatthe prevalence of human herpes virus 8 in peripheral blood mononuclear cells from human immunodeficiency virus infected patients with/without Kaposi sarcoma is probably low in comparison with patients from EE.UUand Europe.

Scientific Electronic Library Online (Spanish)

9

Valoración de las pruebas diagnósticas para tuberculosis bovina en un rebaño bovino ubicado en zona de alta incidencia del estado Zulia, Venezuela/ Valuation of diagnostic tests for bovine tuberculosis in cattle herd located in a high incidence area of Zulia state, Venezuela

Rivera P, Sergio; Jiménez, José Francisco; Deward, Jacobus
2009-12-01

Resumen en español Las pruebas de tuberculina son las de uso generalizado para el diagnóstico y el control de la tuberculosis (TBC) en el hombre y en los animales. Se caracteriza por una compleja mezcla de antígenos de mycobacterias capaces de inducir reacciones de hipersensibilidad en animales infectados, incluso con mycobacterias diferentes al Mycobacterium bovis, por efectos de reactividad cruzada. La preparación del derivado protéico purificado (PPD), es similar a la de la tuberculi (mas) na, a diferencia de la concentración de proteínas, las cuales se separan por precipitación con agentes químicos y no por calor, aumentando su especificidad. Los primeros resultados obtenidos con las pruebas serológicas para el diagnóstico de tuberculosis bovina muestran que existe una gran reactividad antigénica cruzada entre las especies de mycobacterias, por lo que se requiere de antígenos más específicos. Se implementó un ELISA-TBC para la detección de anticuerpos anti M. bovis. El ensayo inmunoenzimático para el IFN-y bovino cuando se utilizó conjuntamente con el sistema de cultivo de sangre completa resultó en un ensayo in vitro rápido y sensible para detectar la reactividad de la inmunidad mediada por células al M. bovis en el ganado infectado. A partir de estas pruebas se compararon los resultados obtenidos para establecer la sensibilidad y especificidad utilizando como prueba oro, los datos obtenidos en el cultivo bacteriológico y la reacción en cadena de la polimerasa (PCR). Los animales reaccionantes a la tuberculina incluyeron animales positivos a PPD-B y PPD-A, así como animales negativos a cultivo bacteriológico y PCR. Los PPD-B positivos, no son en su totalidad, los mismos reaccionantes al IFN-y o al ELISA-TBC. Aún cuando su sensibilidad es baja, muestra mayor especificidad y concordancia que el resto de las pruebas utilizadas. Resumen en inglés The tuberculin tests are widely used for diagnosis and control of tuberculosis (TB) in humans and animals. It is characterized by a complex mixture of mycobacteria antigens able to induce hypersensitivity reactions even in animals infected with mycobacteria other than M. bovis, for purposes of cross-reactivity. The preparation of purified protein derivative (PPD) is similar to the tuberculin, unlike the concentration of proteins which are separated by precipitation with c (mas) hemical agents and not by increasing its specific heat. The first results obtained with the serological tests for diagnosis of bovine tuberculosis show that there is a great antigenic cross-reactivity between mycobacterias species so it requires more specific antigens. It implemented a cattle IFN-g test and ELISA-TBC to detect anti M. bovis activity. The immunoassay test for IFN-g used in conjunction with the cropping system of whole blood resulted in an essay in vitro rapid and sensitive to detect the reactivity of the cell-mediated immunity to M. bovis in livestock infected. Comparative test of the tuberculina, test of Gamma Interferon (INF-y) and a test ELISA-TBC, soon was taken to slaughter house to take linfoides weave samples and nodules, to which the test of chain reaction of Polimerasa was applied to them, to bacteriological culture and (PCR), for the identification from the pathogen. From these tests the patterns of immune response settled down and the obtained results of the different tests were compared to establish sensitivity and specificity using with t gold standard, the data collected in culture and PCR. The results were analyzed using the statistical method of analysis of variance for nonparametric tests. The PPD B-positive, are not the same reacting to IFN-y or at ELISA-TBC. Although its sensitivity is low, it shows greater specificity and consistency as the rest of the tests used.

Scientific Electronic Library Online (Spanish)

10

Identificación de Haemophilus influenzae, Streptococcus pneumoniae y Neisseria meningitidis por reacción en cadena de la polimerasa/ Identification of Haemophilus influenzae, Streptococcus pneumoniae and Neisseria meningitidis by polymerase chain reaction

Parra, Eliana; Castañeda, Elizabeth; Moreno, Jaime
2007-09-01

Resumen en español Introducción. Haemophilus influenzae, Streptococcus pneumoniae y Neisseria meningitidis son los principales patógenos humanos causantes de meningitis. Objetivo. Se evaluaron los iniciadores omp2, lytA y crgA en el desarrollo de una reacción en cadena de la polimerasa múltiple, para la identificación simultánea de los tres principales microorganismos responsables de la meningitis bacteriana. Materiales y métodos. Se evaluaron en un formato de PCR múltiple los inici (mas) adores para la proteína de membrana externa (omp2, 1.000 pb) de H. influenzae, la autolisina A (LytA, 395 pb) de S. pneumoniae y el gen regulador de contacto A (cgrA, 230 pb) de N. meningitidis y se determinó la sensibilidad y la especificidad de la técnica. Resultados. Se obtuvieron resultados reproducibles con una concentración de 50 nM de cada uno de los tres iniciadores y una temperatura de anillamiento de 57°C obteniendo una sensibilidad de 12,5 fg para H. influenzae y S. pnemoniae y de 3,12 fg para N. meningitidis. No se presentaron reacciones cruzadas con otros microorganismos causantes de meningitis o relacionados con los géneros. Conclusión. Los resultados de sensibilidad y especificidad sugieren que los iniciadores evaluados pueden ser utilizados para el desarrollo de una PCR en formato múltiple que permita la identificación de los tres principales patógenos causantes de meningitis. Resumen en inglés Introduction: Haemophilus influenzae, Streptococcus pneumoniae and Neisseria meningitidis are the main human pathogens that cause meningitis. Objective: Primers omp2, lytA and crgA were evaluated with H. influenzae, S. pnumoniae and N. meningitidis DNA in a multiplex PCR, determining the sensitivity and the specificity of the technique. Materials and methods: Primers for H. influenzae outer membrane protein (omp2, 1000 pb), S. pneumoniae autolysin (lytA, 395 pb) and N. me (mas) ningitidis, contact regulated gene (crgA, 230 pb) were evaluated in a multiplex PCR, determining the sensitivity and the specificity of the technique. Results: Reproducible results were obtained with 50 nM of each of the three primers and annealing temperature of 57°C in the multiplex PCR, obtaining a sensitivity of 12.5 fg for H. influenzae and S. pneumoniae and 3.12 fg for N. meningitidis. No cross reactions with other microorganisms agents of meningitis or related with the genera, appeared. Conclusions: The results for sensitivity and specificity suggest that the evaluated primers can be used for the development of a PCR in a multiplex format to the identification of the three main pathogens that cause meningitis.

Scientific Electronic Library Online (Spanish)

11

Variantes alélicas de CYP1A1 y GSTM1 como biomarcadores de susceptibilidad a cáncer gástrico: influencia de los hábitos tabáquico y alcohólico/ Cytochrome P4501A1 (CYP1A1), glutathione S transferase M1 (GSTM1) polymorphisms and their association with smoking and alcohol consumption as gastric cancer susceptibility biomarkers

Lee, Kuen; Cáceres, Dante; Varela, Nelson; Csendes D, Atila; Ríos R, Horacio; Quiñones S, Luis
2006-09-01

Resumen en inglés Background: Gastric cancer (GaC) is the second cause of death by cancer in the world and one of the first causes in Chile. However, the burden of this disease shows remarkable worldwide variation probably explained by environmental and genetic factors. The role of susceptibility low penetrance genes and environmental and dietary factors in the etiology of gastric cancer is not well-known. Aim: To analyze the possible association between CaG susceptibility, genetic (CYP1A1 (mas) and GSTM1 polymorphisms) and environmental (tobacco and alcohol) factors. Patients and Methods: In a case-control study, we included 73 patients with a pathologically diagnosed GaC and 263 controls. DNA was extracted from peripheral blood to detect allele variants for CYP1A1 and GSTM1, using polymerase chain reactions and digestion with restriction enzymes. Results: There was a clear association of smoking and alcohol ingestion with GaC with odds ratios (OR) of 2.54 (95% confidence intervals (CI) of 1.45-4.46 and OR of 3.36 (95% CI 1.76-6.41), respectively. Polymorphic variants of CYP1A1 and GSTM1 had no association with GaC. However, the m2 variant of CYP1A1 significantly modifies the risk induced by tobacco or alcohol (OR 13.65; 95% CI 3.15-59.05 y 8.37; 95% CI 1.86-37.64, respectively). Conclusions: Subjects that carry the m2 allelic variant of CYP1A1 and are exposed to tobacco smoke or alcohol have a significantly higher risk of developing gastric cancer

Scientific Electronic Library Online (Spanish)

14

Tyrosinase gene expression is not detected in mouse brain outside the retinal pigment epithelium cells

Giménez, Estela; Lavado, Alfonso J.; Giraldo, Patricia; Montoliu, Lluís
2003-10-27

Digital.CSIC (Spain)

17

The mechanism of precipitation of chain-like calcite

García Carmona, Jesús; Gómez-Morales, J.; Fraile Sáinz, Julio; Loste, Eva; Rodríguez Clemente, Rafael
2004-02-15

Digital.CSIC (Spain)

18

The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes

Ramos Escribano, Javier; Matamoros Galindo, Manuel Antonio; Naya, Loreto; James, Euan K.; Rouhier, Nicolas; Sato, Shusei; Tabata, Satoshi; Becana Ausejo, Manuel
2009-01-01

Digital.CSIC (Spain)

19

The Prestige oil spill: bacterial community dynamics during a field biostimulation assay

Jiménez, Núria; Viñas, Marc; Bayona Termens, Josep María; Albaigés Riera, Joan; Solanas, Anna M.
2007-10-18

Digital.CSIC (Spain)

21

Synthesis, characterization and optical properties of merocyanines derived from malononitrile dimer

Alías, Silvia; Andreu, Raquel; Cerdán, Miguel Ángel; Franco, Santiago; Garín, Javier; Orduna, Jesús; Romero, Pilar; Villacampa, Belén
2007-09-10

Digital.CSIC (Spain)

23

Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase

Günther Sillero, María A.; Guranowski, Andrzej; Sillero, Antonio
1991-12-01

Digital.CSIC (Spain)

24
25

Synthesis and structural characterisation of new mono and dinuclear pyrazolatopalladium (II) complexes self-assembled through robust hydrogen-bonds

Ara, Irene; Falvello, Larry R.; Forniés, Juan; Lasheras, Roberto; Martín, Antonio; Oscar Oliva, Ocar; Sicilia, Violeta
2006-11-01

Digital.CSIC (Spain)

26

Synthesis and crystal structure of the monoclinic modification of Yb(ReO4)3(H2O)4

Mujica, Carlos; Llanos, Jaime; Sánchez, Víctor; Gómez-Romero, P.; Casañ Pastor, Nieves
2003-04-01

Digital.CSIC (Spain)

27

Synthesis and Spectral Properties of Amphiphilic Lipids with Linear Conjugated Polyene and Phenylpolyene Fluorescent Groups

Quesada, Ernesto; Delgado, Javier; Hornillos, Valentín; Acuña Fernandez, Alberto Ulises; Amat-Guerri, Francisco
2007-03-20

Digital.CSIC (Spain)

28

Stereoselective synthesis of amino acid-derived β-lactams. Experimental evidence for TADDOL as a memory of chirality enhancer

Bonache de Marcos, María Ángeles; López, Pilar; Martín Martínez, María Mercedes; García-López, María Teresa; Cativiela, Carlos; González-Muñiz, Rosario
2006-01-01

Digital.CSIC (Spain)

29

Stereoselective opening of ring E in furostan sapogenins. An efficient route to 16,22R,26-hydroxy steroids

González, Antonio G.; Freire, Raimundo; Francisco, Cosme G.; Salazar, José A.; Hernández, Rosendo; Suárez, Ernesto
1974-01-01

Digital.CSIC (Spain)

30

Snail family transcription factors are implicated in thyroid carcinogenesis.

Hardy, R. G.; Vicente-Dueñas, C.; González-Herrero, I.; Anderson, C.; Flores, T.; Hughes, S.; Ross, J. A.; Sánchez-García, I.; Tselepis, C.
2007-01-01

Digital.CSIC (Spain)

33

Sex pheromone biosynthetic pathway for disparlure in the gypsy moth, Lymantria dispar

Jurenka, Russell A.; Subchev, Mitko; Abad, José Luis; Choi, Man-Yeon; Fabriàs, Gemma
2003-01-17

Digital.CSIC (Spain)

37

Regulation of surface hydrophilicity of plasma treated wool fabrics

Canal, Cristina; Erra Serrabasa, Pilar; Molina Mansilla, Ricardo; Bertrán, Enric
2007-08-01

Digital.CSIC (Spain)

38

Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production

Fernández García, María; Martínez Linares, Daniel; Río Lagar, Beatriz del; Martín, M. Cruz; Álvarez González, Miguel Ángel
2006-01-01

Digital.CSIC (Spain)

39

Quantitative monitoring of colonization of olive genotypes by Verticillium dahliae pathotypes with real-time polymerase chain reaction

Mercado-Blanco, Jesús; Collado-Romero, M.; Parrilla-Araujo, S.; Jiménez-Díaz, Rafael M.
2003-08-01

Digital.CSIC (Spain)

41

Purification, N-terminal sequencing and diagnostic value of the major antigens of Ornithodoros erraticus and O. moubata

Baranda, José A.; Pérez Sánchez, Ricardo; Oleaga, Ana; Manzano Román, Raúl; Encinas Grandes, Antonio
2000-01-01

Digital.CSIC (Spain)

42

Profile of P-glycoprotein distribution in the rat and its possible influence on the salbutamol intestinal absorption process

Valenzuela, Belén; Nácher, Amparo; Ruiz-Carretero, Purificación; Martín-Villodre, Adela; López-Carballo, Gracia; Barettino, Domingo
2004-04-15

Digital.CSIC (Spain)

43

Potential microbial risk factors related to soil amendments and irrigation water of potato crops

Selma, María Victoria; Allende, Ana; López-Gálvez, Francisco; Elizaquível, P.; Aznar, Rosa; Gil Muñoz, M.ª Isabel
2007-08-04

Digital.CSIC (Spain)

44

Polirradiculopatía por citomegalovirus en 2 pacientes con SIDA: tratamiento exitoso con terapia anti retroviral de alto grado de actividad (TAAGA)/ Polyradiculopathy caused by cytomegalovirus in 2 aids patients treated successfully with highly active antiretroviral therapy (HAART)

Lasso B, Martín; Pérez G, Jorge; Noriega R, Luis M; Albert S; González A, Patricia; Malebrán R, Andrea
2001-09-01

Resumen en inglés We report two male patients with AlDS, aged 23 and 30 years old respectively. One was admitted due to a progressive weakness of the left leg and urinary and fecal incontinence. The other was admitted due to a progressive paraparesia without incontinence, but with a severe lumbar pain. In both patients polymerase chain reactions for cytomegalovirus in cerebrospinal fluid were positive. Treatment with ganciclovir, for 21 and 14 days respectively and highly active antiretrov (mas) iral therapy was started. Both patients experienced recovery of their neurological deficits after 98 and 88 days of therapy, respectively. (Rev Méd Chile 2001; 129: 1061-4)

Scientific Electronic Library Online (Spanish)

45

Plant-Parasitic nematodes infecting grapevine in southern Spain and susceptible reaction to root-knot nematodes of rootstocks reported as moderately resistant

Téliz, D.; Landa, Blanca B.; Rapoport, Hava F.; Perez Camacho, F.; Jiménez-Díaz, Rafael M.; Castillo, Pablo
2007-09-01

Digital.CSIC (Spain)

46

Photodetachment spectrum of OHF-: Three-dimensional study of the heavy–light–heavy resonances

González-Sánchez, Lola ; Gómez Carrasco, Susana ; Aguado, Alfredo ; Paniagua, Miguel ; Hernández, M. Luz

12 pages, 14 figures, 1 table.-- PACS nrs.: 82.30.Cf; 82.20.Kh; 31.15.Ar; 33.15.Mt; 33.80.Gj; 31.25.Eb; 31.50.Bc. | In this work a simulation of the OHF- photodetachment spectrum is performed in a three-dimensional potential energy surface recently developed for OHF(3A''). The ground 2A' state poten...

DRIVER (Spanish)

47

Photodetachment spectrum of OHF-: Three-dimensional study of the heavy–light–heavy resonances

González-Sánchez, Lola; Gómez Carrasco, Susana; Aguado, Alfredo; Paniagua, Miguel; Hernández, M. Luz; Alvariño, José M.; Roncero, Octavio
2004-07-01

Digital.CSIC (Spain)

48

Peroxisomal Monodehydroascorbate Reductase. Genomic Clone Characterization and Functional Analysis under Environmental Stress Conditions

Leterrier, Marina; Corpas, Francisco J.; Barroso, Juan B.; Sandalio, Luisa M.; Río, Luis A. del
2005-08-01

Digital.CSIC (Spain)

49

Optical, Redox, and NLO Properties of Tricyanovinyl Oligothiophenes: Comparisons between Symmetric and Asymmetric Substitution Patterns

Casado, Juan; Ruiz Delgado, M. Carmen; Rey Merchán, M. Carmen; Hernández, Víctor; López Navarrete, Juan T.; Pappenfus, Ted M.; Williams, Nathaniel; Stegner, William J.; Johnson, Jared C.; Edlund, Brett A.; Janzen, Daron E.; Mann, Kent R.; Orduna, Jesús; Villacampa, Belén
2006-04-21

Digital.CSIC (Spain)

51

New Insight into the Gas-Phase Bimolecular Self-Reaction of the HOO Radical

Anglada Rull, Josep M.; Olivella, Santiago; Sol, Albert
2007-10-02

Digital.CSIC (Spain)

52

Multiplex Fast Real-Time Polymerase Chain Reaction for quantitative detection and identification of cos and pac Streptococcus thermophilus bacteriophages

Río Lagar, Beatriz del; Martín Martín, M. Cruz; Martínez, Noelia; Hernández Magadán, Alfonso; Álvarez González, Miguel Ángel
2008-06-06

Digital.CSIC (Spain)

54

Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes

Almoguera, Concepción; Shibata, D.; Forrester, K.; Martin, J.; Amheim, N.; Perucho, M.
1988-01-01

Digital.CSIC (Spain)

56

Monitoring of HAB species in the Mediterranean Sea through molecular methods

Penna, Antonella; Bertozzini, Elena; Battocchi, Cecilia; Galluzzi, Luca; Giacobbe, Maria Grazia; Vila, Magda; Garces, Esther; Lugliè, Antonella; Magnant, Mauro
2007-01-01

Digital.CSIC (Spain)

57

Molecular variability within and among Verticillium dahliae vegetative compatibility groups determined by fluorescent amplified fragment length polymorphism and polymerase chain reaction markers

Collado-Romero, M.; Mercado-Blanco, Jesús; Olivares-García, Concepción; Valverde-Corredor, Antonio; Jiménez-Díaz, Rafael M.
2006-05-01

Digital.CSIC (Spain)

58

Molecular methods for detecting guar additions to locust bean gum.

Benedí Benito, Vicente Javier; Domenéch Sanchez, Antonio; Hernández, M.; Alberti Serrano, Sebastian; Roselló, Josep A.
2001-09-13

Digital.CSIC (Spain)

60

Molecular cloning of Senegalese sole (Solea senegalensis) follicle-stimulating hormone and luteinizing hormone subunits and expression pattern during spermatogenesis

Cerdà, Joan; Chauvigné, François; Agulleiro, María J.; Marin, Elena; Halm, Silke; Martínez-Rodríguez, Gonzalo; Prat, Francisco
2008-05-01

Digital.CSIC (Spain)

61

Molecular cloning and pattern of expression of an alpha-L-fucosidase gene from pea seedlings

Augur, Christopher; Stiefel, Virginia; Darvin, Alan; Albersheim, Peter; Puigdomènech, Pere
1995-10-01

Digital.CSIC (Spain)

62

Molecular and functional characterization of voltage-gated sodium channels in human sperm

Pinto Pérez, Francisco M.; Ravina, Cristina G.; Fernández-Sánchez, Manuel; Gallardo-Castro, Manuel; Cejudo-Román, Antonio; Candenas de Luján, M. Luz
2009-07-16

Digital.CSIC (Spain)

63

Molecular Characterization of Meloidogyne hispanica (Nematoda, Meloidogynidae) by Phylogenetic Analysis of Genes Within the rDNA in Meloidogyne spp.

Landa, Blanca B.; Palomares Rius, Juan E.; Vovlas, Nicola; Carneiro, Regina M. D. G.; Maleita, Carla M. N.; Abrantes, Isabel M. de O.; Castillo, Pablo
2008-07-01

Digital.CSIC (Spain)

65

Molecular Carbon Chains and Rings in TMC-1

Cernicharo, José; Fosse, David; Gerin, M.; Cox, Pierre
2000-12-19

Digital.CSIC (Spain)

66

Modification of the pyrolysis/carbonization of PPTA polymer by intermediate isothermal treatments

Castro Muñiz, Alberto; Martínez Alonso, Amelia; Díez Tascón, Juan Manuel
2008-03-07

Digital.CSIC (Spain)

68

Mechanism of molecular hydrogen dissociation on gold chains and clusters as model prototypes of nanostructures

Zanchet, Alexander; Dorta-Urra, A.; Roncero, Octavio; Flores, F.; Tablero, C.; Paniagua, Miguel; Aguado, Alfredo
2009-01-01

Digital.CSIC (Spain)

69

Los genotipos de Helicobacter pylori en gastritis no atrófica difieren de los encontrados en úlcera péptica, lesiones premalignas y cáncer gástrico en Colombia/ Helicobacter pylori genotypes in non atrophic gastritis, peptic ulcer disease, premalignant lesions and gastric cancer in Colombia

Cittelly P, Diana M; Huertas, Mónica G; Martínez, Julián D; Oliveros, Ricardo; Posso, Héctor; Bravo, María Mercedes; Orozco D, Oscar
2002-02-01

Resumen en inglés Background: Helicobacter pylori is recognized as an etiologic agent of several gastric diseases. Bacterial genotypes have been related to clinical outcome in several populations. Aim: To compare cagA, vacA and iceA genotypes of Colombian isolates from patients with several gastrointestinal diseases, including gastric cancer. Material and methods: We used polymerase chain reactions to amplify vacA, cagA and iceA genes of 137 H pylori isolates coming from 26 patients with g (mas) astric cancer (GC), 34 with peptic ulcer (PU), 19 with intestinal metaplasia (IM), 23 with atrophic gastritis (AG) and 35 with non atrophic gastritis (NAG). Results: vacA s1-m1, cagA+, iceA+ were the most frequently found genotypes. vacA s1 and m1 subtypes were found in 92 (67%) and 82 (60%) cases respectively. Sixty three percent were cagA+ and 85% were iceA+. There was a lower prevalence of s1 allele in cases of NAG (43%), compared with GC, PU and IM (81%, 77% and 81% prevalence, respectively, p

Scientific Electronic Library Online (Spanish)

70

Long-acting contraceptive agents: testosterone esters of unsaturated acids

Francisco, Cosme G.; Freire, Raimundo; Gawronski, J.; Hernández, Rosendo; Kielczewski, M.; Salazar, José A.; Savabi, F.; Shafiee, A.; Strekowski, L.; Suárez, Ernesto
1990-01-01

Digital.CSIC (Spain)

71

Linkage of 35S and 5S rRNA genes in Artemisia (family Asteraceae): first evidence from angiosperms

Garcia Giménez, Sònia; Lim, K.Y.; Chester, M.; Garnatje, Teresa; Pellicer, J.; Vallès, Joan; Leitch, A.R.; Kovaric, Ales
2009-01-01

Digital.CSIC (Spain)

72

La suplementación con hierro y el aumento del estrés oxidativo en el embarazo: una paradoja poco discutida/ Iron supplementation and oxidative stress in pregnancy: a little-discussed paradox

Calderón-Vélez, Juan Camilo
2007-12-01

Resumen en español La suplementación con hierro a las mujeres embarazadas es una recomendación ampliamente aceptada en la práctica médica. Sin embargo, el hierro participa en los fenómenos de iniciación y propagación de reacciones de generación de especies reactivas de oxígeno e hidroperóxidos lipídicos, los cuales juegan un papel importante en la fisiopatología de enfermedades como diabetes gestacional y preeclampsia. Aparece una paradoja: suplementar con hierro disminuye el ri (mas) esgo de anemia y complicaciones, pero esta podría favorecer el desarrollo de otras enfermedades. Esta paradoja se discute en el texto y se sugieren estudios que se deben realizar para aclarar cuáles son las condiciones de mayor seguridad para suplementar con hierro a las mujeres gestantes. Resumen en inglés Providing iron supplement during pregnancy is a widely accepted medical practice. However, iron is involved in initiation and propagation chain reactions producing reactive oxygen species and lipid hydroperoxides, in turn playing an important role in the pathophysiology of gestational diabetes and preeclampsia. A paradox is then faced; iron supplement can reduce the risk of anaemia and anaemia-related complications but it can promote other diseases developing. Such parado (mas) x is discussed and some studies are suggested which could be carried out to clarify when iron supplement may be indicated and used in the safest conditions for pregnant women.

Scientific Electronic Library Online (Spanish)

73

Kinetic characterization of the substrate specificity and mechanism of mushroom tyrosinase

Espín de Gea, Juan Carlos; Varón, Ramón; Fenoll, Lorena G.; Gilabert, M. Ángeles; García-Ruíz, Pedro; Tudela, José; García-Cánovas, Francisco
2000-02-01

Digital.CSIC (Spain)

74

Identificación de mutaciones en el gen CFTR en pacientes chilenos con fibrosis quística/ Identification of cystic fibrosis transmembrane regulator (CFTR) mutations in Chilean patients with cystic fibrosis

Repetto L, Gabriela; Poggi M, Helena; Harris D, Paul; Navarro M, Héctor; Sánchez D, Ignacio; Guiraldes C, Ernesto; Pérez H, M Angélica; Boza R, M Lina; Hunter M, Bessie; Wevar C, M Elena; Mediavilla R, Marisol; Foradori C, Arnaldo
2001-08-01

Resumen en inglés Background: Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the CFTR gene, that codes for a chloride channel located in the apical surface of epithelial cells. The main role of this protein is the regulation of chloride transport, and secondarily, of sodium and water to the extracellular space. More than 900 gene mutations have been described, and their relative frequency in different populations depends on their ethnic origin. Aim: To report (mas) the findings of Chilean patients with cystic fibrosis, in whom the presence of 20 common mutations was analyzed. Patients and methods: Fifty seven patients with established diagnosis or suspicion of CF were studied. The simultaneous identification of 20 mutations and the normal deltaF508 allele was done using polymerase chain reactions with a commercial assay. Results: Eight mutations were found. Fifty patients fulfilled diagnostic criteria proposed by the Consensus Panel of the CF Foundation and 66% of alleles were identified in this group. ∆F508 mutation was found in 45%. We did not identify mutations in any of the remaining 7 patients. Conclusions: Our results suggest that the majority of undetected mutations are associated with atypical phenotypes or that some patients in this series could have other diseases. We recommend to include mutation analysis in the evaluation of Chilean patients with CF. It is useful to establish prognosis and genetic counselling (Rev Méd Chile 2001; 129: 841-7)

Scientific Electronic Library Online (Spanish)

75

INMUNOGENICIDAD DE LA PROTEÍNA RECOMBINANTE ASP1R DE Ancylostoma caninum EN UN MODELO MURINO/ IMMUNOGENICITY OF THE PROTEIN RECOMBINANT ASP1R OF A caninum IN A MURINE MODEL

Giraldo G, María; Castaño O, Jhon C
2009-08-01

Resumen en español Objetivo. Construir un plásmido recombinante que exprese la proteína ASP1r de Ancylostoma caninum y evaluar su capacidad inmunogénica en un modelo murino. Materiales y métodos. Se realizó extracción de ARN de parásitos adultos de Ancylostoma caninum, se amplificó por RT-PCR el gen de la proteína ASP1. Este gen fue insertado en el vector pcDNA3. El inserto fue digerido con Bamh1 y EcoR1 y clonado direccionalmente. Posteriormente, se llevó a cabo transformación y (mas) selección de las células de E. coli DH5a competentes con el producto de la ligación. Se realizó un tamizaje por PCR confirmando la presencia del gen ASP1. El vector pcDNA3-ASP1 fue administrado vía intraglandular en la parotida e intramuscular en ratones Balb/c. En estos animales se les realizó determinación de anticuerpos en suero y saliva mediante las técnicas de ELISA e inmunohistoquímica. Resultados. Se determinó que el plásmido pcDNA3-ASP1 fue incorporado y expresado células E. coli DH5a. Este plásmido recombinante indujo la producción de anticuerpos Anti-ASP1 específicos en ratones Balb/c. Conclusiones. Se logró demostrar que la utilización de pcDNA3-ASP1 no produjo reacciones desfavorables en ratones Balb/c, además indujo respuesta humoral contra la proteína pcDNA3-ASP1 de excreción/secreción de Ancylostoma caninum en ratones. Resumen en inglés Objective. To construct a recombinant plasmid expressing the ASP1r protein of A. caninum and evaluate its immunogenic capacity in a murine model. Materials and methods. RNA of adults of Ancylostoma caninum was amplified by reverse transcription polymerase chain reaction. The ASP1 protein gene was inserted into the pcDNA3 vector. Plasmid was digested with Bamh1 and EcoR1 and cloning was performed directionally. Later a transformation and selection of E. coli DH5a cell comp (mas) etent with the product for the ligation was made. Then, a screening by PCR was carried out to confirm the presence of ASP1 gene. PcDNA3-ASP1 was inoculated by intraglandular parotide and intramuscular route in Balb/c mice. Antibodies in these animals was measured in serum and saliva by ELISA and immunochemistry. Results. PcDNA3-ASP1 was incorporated and expressed in E. coli DH5a cell. This recombinant plásmid was able to produce antibodies anti-ASP1 specific in Balb/c mice. Discussion. It was possible to demonstrate that using of pcDNA3-ASP1 did not display reactogenicity and it did not produce unfavorable reactions, futhermore, it induced a humoral response against the excreción/secretion protein of Ancylostoma caninum in mice.

Scientific Electronic Library Online (Spanish)

77

Host-parasite relationships in fall-sown sugar beets infected by the stem and bulb nematode, Ditylenchus dipsaci

Castillo, Pablo; Vovlas, Nicola; Azpilicueta, A.; Landa, Blanca B.; Jiménez-Díaz, Rafael M.
2007-01-01

Digital.CSIC (Spain)

79

Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1/ Helicobacter pylori: cagA status and vacA genotyping in Chile. Detection of a s2/m1 strain

Martínez T, Alejandra; González C, Carlos; Kawaguchi P, Fernando; Montoya, Rolando; Corvalán, Alejandro; Madariaga B, Jaime; Roa S, Jorge; García C, Apolinaria; Salgado, Fernando; Solar, Henry; Palma, Mariana
2001-10-01

Resumen en inglés Background: The genes cagA and vacA encode H pylori virulence factors. Aim: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material and methods: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA gerotyping was done using polymerase chain reactions. Results: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s (mas) 2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. Conclusions: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2 (Rev Méd Chile 2001; 129: 1147-53

Scientific Electronic Library Online (Spanish)

80

Genetic procedures for identification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks

Martín, M. Cruz; Fueyo Mendoza, José María; González-Hevia, M. A.; Mendoza, M. Carmen
2004-08-01

Digital.CSIC (Spain)

81

Fischer-Tropsch synthesis on mono- and bimetallic Co and Fe catalysts in fixed-bed and slurry reactors

Peña O'Shea, Víctor A. de la; Álvarez Galván, María Consuelo; Campos Martín, Jose Miguel; García Fierro, José Luis
2007-04-06

Digital.CSIC (Spain)

82

First report of Meloidogyne arenaria parasitizing lettuce in southern Spain

Castillo, Pablo; Landa, Blanca B.; Navas Cortés, Juan Antonio; Vovlas, Nicola; Jiménez-Díaz, Rafael M.
2006-07-01

Digital.CSIC (Spain)

83

F+OH reactive collisions on new excited 3A'' and 3A' potential-energy surfaces

Gómez Carrasco, Susana ; Roncero, Octavio ; González-Sánchez, Lola ; Hernández, M. Luz ; Alvariño, José M.

13 pages, 10 figures, 5 tables.-- PACS nrs.: 82.30.Cf; 82.20.Kh; 82.20.Db; 82.20.Pm. | Global three-dimensional adiabatic potential-energy surfaces for the excited 2(3)A'' and 1(3)A' triplet states of OHF are obtained to study the F(2P)+OH(2Π)O(3P)+HF(1Σ+) reaction. Highly accurate ab initio calcula...

DRIVER (Spanish)

84

F+OH reactive collisions on new excited 3A'' and 3A' potential-energy surfaces

Gómez Carrasco, Susana; Roncero, Octavio; González-Sánchez, Lola; Hernández, M. Luz; Alvariño, José M.; Paniagua, Miguel; Aguado, Alfredo
2005-09-22

Digital.CSIC (Spain)

85

Estudio de tres loci hipervariables en población mixta chilena/ Study of three hypervariable loci in a mixed Chilean population

Novoa C, M del Rosario; Labbé C, Carolina; Jorquera G, Hugo; Moreno Ch, Fabián; Aguirre M, M Eugenia; Cifuentes O, Lucía
2001-01-01

Resumen en inglés Background: Genetic markers are useful to study evolution parameters in populations and to determine kinship. Aim: To characterize three short tandem repeat loci in a sample of Chilean subjects and compare them with Caucasian and Hispanic populations. Material and methods: Three hundred ninety three unrelated subjects that were sent for genetic studies from courts of justice, were studied. The loci FESFPS, F13A01 and vWA in blood samples, were typified amplifying DNA by p (mas) olymerase chain reactions. Results: The three studied loci were highly polymorphic. F13A01 and FESFPS were in Hardy-Weinberg genetic equilibrium. A significant excess of heterozygotes was detected for vWA locus. There were no differences in allele frequencies, according to ethnic origins of last names. Allele frequencies for F13A01 and vWA loci were similar to those of Hispanic populations of Unites States and FESFPS loci was different. Conclusions: All three loci had a high efficiency for genetic identification tests according to the estimated a priory exclusion probability (Rev Méd Chile 2001; 129: 75-79)

Scientific Electronic Library Online (Spanish)

86

Enzymatic modification for ascorbic acid and alpha-tocopherol to enhance their stability in food and nutritional applications

Torres, Pamela; Kunamneni, Adinarayana; Ballesteros, Antonio; Plou Gasca, Francisco José
2008-01-01

Digital.CSIC (Spain)

87

Enzymatic desaturation of fatty acids: delta11 desaturase activity on cyclopropane acid probes

Villorbina, Gemma; Roura, Lidia; Camps Díez, Francisco; Joglar Tamargo, Jesús; Fabriàs, Gemma
2003-03-06

Digital.CSIC (Spain)

88

Ehrlichiosis humana en Chile: evidencia serológica/ Serologic evidence for human Ehrlichiosis in Chile

López D, Javier; Rivera S, Marisol; Concha G, Juan Carlos; Gatica Q, Silvana; Loeffeholz, Mike; Barriga V, Omar
2003-01-01

Resumen en inglés Background: Ehrlichiosis is a non contagious infectious disease, mainly transmitted by thick bites. In 1998, this infection was detected in dogs, for the first time, in Chile. Aim: To establish if there is human exposure to Ehrlichia sp in Chile. Material and methods: Blood samples from 17 dogs with ehrlichiosis and 19 humans who had contact with them were studied to determine human exposure to Ehrlichia equi and Ehrlichia chaffeensis in Chile. Samples were analyzed by in (mas) direct inmunofluorecence and by polymerase chain reaction (PCR). Results: Six dogs had positive titers against both species of ehrlichia; 2 with titers of 1/256; 3 with titers over 1/512 to Ehrlichia equi and titers of 1/256, 1/128 and 1/64 to Ehrlichia chaffeensis respectively, and 1 with titers of 1/256 to Ehrlichia equi and titers of 1/128 to Ehrlichia chaffeensis. Two of the 19 humans, had positive titers against both antigens (1/128). PCR reactions were negative in both human and canine sera. Conclusions: These results confirm that human exposure to Ehrlichia sp. Epidemiological surveillance for human ehrlichiosis should be implemented in the country (Rev Méd Chile 2003; 131: 67-70)

Scientific Electronic Library Online (Spanish)

89

Effects of salt stress on the expression of antioxidant genes and proteins in the model legume Lotus japonicus

Rubio Luna, Mari Carmen; Bustos-Sanmamed, Pilar; Clemente, María Rebeca; Becana Ausejo, Manuel
2009-03-01

Digital.CSIC (Spain)

91

Distribución de la punta morada y Bactericera cockerelli Sulc. en las principales zonas productoras de papa en México/ Distribution of potato purple top and Bactericera cockerelli Sulc. in the main potato production zones in Mexico

Rubio Covarrubias, Oswaldo Ángel; Almeyda León, Isidro Humberto; Ireta Moreno, Javier; Sánchez Salas, José Alfredo; Fernández Sosa, Rogelio; Borbón Soto, José Trinidad; Díaz Hernández, Carlos; Garzón Tiznado, José Antonio; Rocha Rodríguez, Ramiro; Cadena Hinojosa, Mateo Armando
2006-08-01

Resumen en español Los síntomas de la punta morada son provocados por fitoplasmas, cuyos vectores son varias especies de chicharritas (Homoptera: Cicadelidae) y el psílido de la papa Bactericera cockerelli Sulc. (Hemiptera: Triozidae). Síntomas similares pueden ser ocasionados por una toxina que el psílido de la papa inyecta al alimentarse en el floema de la planta. Con el objeto de evaluar la importancia y distribución de la punta morada de la papa y de sus vectores, en 2001 se realiz (mas) ó un muestreo de plantas que presentaban los síntomas de la enfermedad en las principales zonas productoras de papa en México. En cada área seleccionada se colocaron trampas amarillas con pegamento para capturar especímenes de B. cockerelli y de chicharritas. Las plantas seleccionadas fueron sometidas a un análisis de fitoplasmas con la técnica de la Reacción en Cadena de la Polimerasa. Se encontraron los siguientes porcentajes de muestras positivas: Estado de México 29%, Coahuila-Nuevo León 50%, Tlaxcala 17%, Guanajuato 31%, Jalisco 75% y Sonora 10%. B. cockerelli y chicharritas estuvieron presentes en todas las zonas paperas, con excepción de Tapalpa, Jalisco, en donde no se encontró B. cockerelli y la población de chicharritas fue baja. Las altas poblaciones de los insectos vectores de la punta morada, en la mayor parte de las zonas productoras de papa, y los altos porcentajes (10-75%) de las muestras encontradas con fitoplasmas en las áreas de estudio indican la importancia que tiene este problema para la producción de papa en México. Resumen en inglés Symptoms of potato purple top are caused by phytoplasmas transmitted by leafhoppers (Homoptera: Cicadelidae) and the potato psyllid Bactericera cockerelli Sulc. (Hemiptera: Triozidae). Similar symptoms may also be caused by a toxin that the potato psyllid injects when it is feeding from the phloem sap. To determine the importance and distribution of the potato purple top disease in Mexico, a field sampling was conducted in 2001 in the main potato producing areas. In each (mas) area, plants with the disease symptoms were sampled to investigate the presence of phytoplasmas by using the polymerase chain reactions technique. Yellow sticky insects traps were installed in each potato producing area and trapped leafhoppers and B. cockerelli counted. The percentages of the plant samples that were positive in each potato area were: State of Mexico 29%, Coahuila-Nuevo Leon 50%, Tlaxcala 17%, Guanajuato 31%, Jalisco 75% and Sonora 10%. Leafhoppers were present in most of the potato regions, only in the high lands of Tapalpa, Jalisco area the leafhopper populations was low and B. cockerelli was not found. The high population of the disease vectors found in most potato producing areas in Mexico and the high percentages (10-75%) of the plant samples infected by phytoplasmas indicate their importance on restraining the potato production in Mexico.

Scientific Electronic Library Online (Spanish)

92

Disentangling Vector-Borne Transmission Networks: A Universal DNA Barcoding Method to Identify Vertebrate Hosts from Arthropod Bloodmeals

Alcaide, Miguel; Rico, Ciro; Ruiz, Santiago; Soriguer, Ramón C.; Muñoz, Joaquín; Figuerola, Jordi
2009-09-21

Digital.CSIC (Spain)

93

Direct versus resonances mediated F+OH collisions on a new 3A'' potential energy surface

Gómez Carrasco, Susana ; González-Sánchez, Lola ; Aguado, Alfredo ; Roncero, Octavio ; Alvariño, José M.

14 pages, 13 figures, 4 tables.-- PACS nrs.: 82.30.Cf; 82.20.Kh; 31.15.Ar; 82.20.Fd; 33.80.Eh; 31.50.Df. | A theoretical study of the F(2P) + OH(2Π)HF(1Σ+) + O(3P) reactive collisions is carried out on a new global potential energy surface (PES) of the ground 3A'' adiabatic electronic state. The ab ...

DRIVER (Spanish)

94

Direct versus resonances mediated F+OH collisions on a new 3A'' potential energy surface

Gómez Carrasco, Susana; González-Sánchez, Lola; Aguado, Alfredo; Roncero, Octavio; Alvariño, José M.; Hernández, M. Luz; Paniagua, Miguel
2004-09-08

Digital.CSIC (Spain)

96

Differentiation of cotton-defoliating and nondefoliating pathotypes of Verticillium dahliae by RAPD and specific PCR analyses

Pérez-Artés, Encarnación; García-Pedrajas, María D.; Bejarano-Alcázar, J.; Jiménez-Díaz, R. M.
2000-01-01

Digital.CSIC (Spain)

98

Differential expression of genes mapping to recurrently abnormal chromosomal regions characterize neuroblastic tumours with distinct ploidy status

Lavarino, Cinzia; Garcia, Idoia; Mackintosh, Carlos; Cheung, Nai-Kong V.; Domenech, Gema; Ríos, José; Perez, Noelia; Rodríguez, Eva; Torres, Carmen de; Gerald, William L.; Tuset, Esperanza; Acosta, Sandra; Beleta, Helena; Álava, Enrique de; Mora, Jaume
2008-08-13

Digital.CSIC (Spain)

101

Detection of c-K-ras mutations in fine needle aspirates from human pancreatic adenocarcinomas

Shibata, D.; Almoguera, Concepción; Forrester, K.; Dunitz, J.; Martin, S.E.; Cosgrove, M.M.; Perucho, M.; Arnheim, N.
1990-02-01

Digital.CSIC (Spain)

102

Detection of C3O in IRC+10216: Oxygen-Carbon chain chemistry in the outer envelope

Tenenbaum, E. D.; Apponi, A. J.; Ziurys, L. M.; Agúndez, Marcelino; Cernicharo, José; Pardo Carrión, Juan Ramón; Guélin, Michel
2006-09-20

Digital.CSIC (Spain)

103

Detection and identification of phytoplasma DNA in symptomatic mushrooms of the genus Ramaria (O. Gomphales)

Martín, María Paz; Pérez Daniëls, Pablo; Torres, Ester; Tellería, M.ª Teresa
2002-06-30

Digital.CSIC (Spain)

104
105

Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica/ Detection of pyrogenic exotoxin SpeA, SpeB and SpeC genes in Chilean streptococci isolates and their association with clinical manifestations

Ulloa F, María Teresa; Giglio M, María Soledad; Porte T, Lorena; Santa Cruz A, Adriana; McNab M, Paul; Fica C, Alberto; Pinto C, María Eugenia; Kawaguchi G, Keyko; Carmi K, Alejandra
2000-01-01

Resumen en inglés Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Streptococcus pyogenes strains and their association with the invasiveness of infections. Material and methods: The genes for pyrogenic exotoxins SpeA, SpeB and SpeC were determined (mas) by polymerase chain reactions in 114 strains of group A Streptococcus pyogenes isolated from Chilean patients with invasive or non invasive infections. Results: The gene for SpeA was present in 30.7% of isolates, the gene for SpeB was present in 69.3% and the gen for SpeC in 44.7% of isolates. The gene for SpeA was present in 20 of 33 invasive infections and in 15 of 81 non invasive infections (p <0.0001). On the contrary, the gene for SpeC was present in 11 of 33 invasive infections and in 41 of 81 non invasive infections (p <0.05). The frequency of speB was similar in invasive and non invasive infections. Conclusions: There is a clear relationship between the presence of SpeA genes and the severity of infections caused by Streptococcus pyogenes. (Rev Méd Chile 2000; 128: 27-34)

Scientific Electronic Library Online (Spanish)

106

DFT Research on the Dehydroxylation Reaction of Pyrophyllite 1. First-Principle Molecular Dynamics Simulations.

Molina-Montes, Esther; Donadio, Davide; Hernández-Laguna, Alfonso; Sainz-Díaz, C. Ignacio; Parrinello, Michele
2008-05-20

Digital.CSIC (Spain)

107

Coupled diabatic potential energy surfaces for studying the nonadiabatic dynamics at conical intersections in angular resolved photodetachment simulations of OHF- --> OHF+e-

Gómez Carrasco, Susana ; Aguado, Alfredo ; Paniagua, Miguel ; Roncero, Octavio

16 pages, 14 figures, 1 table.-- PACS nrs.: 82.20.Kh; 82.30.Cf; 33.80.Eh; 82.20.Db; 82.20.Hf. | An energy-based method is proposed for the diabatization of the OH(2Π)+F(2P) --> O(3P)+HF(1Σ+) reaction. It is demonstrated that the diabatic representation obtained is regularized, i.e., the residual der...

DRIVER (Spanish)

109

Coordinate transformation methods to calculate state-to-state reaction probabilities with wave packet treatments

Gómez Carrasco, Susana ; Roncero, Octavio

14 pages, 13 figures, 2 tables, 1 appendix.-- PACS nrs.: 82.30.Cf; 82.20.Rp. | A procedure for the transformation from reactant to product Jacobi coordinates is proposed, which is designed for the extraction of state-to-state reaction probabilities using a time-dependent method in a body-fixed frame...

DRIVER (Spanish)

111

Control of the chemical structure of perhydrous coals. FTIR and Py-GC/MS investigations

Iglesias, María José; Río Andrade, José Carlos del; Laggoun-Défarge, Fatima; Cuesta, María José; Suárez Ruiz, Isabel
2002-01-01

Digital.CSIC (Spain)

112

Combining microsatellite markers and capillary gel electrophoresis with laser-induced fluorescence to identify the grape (Vitis vinifera) variety of musts

Rodríguez-Plaza, P.; González, Ramón; Moreno-Arribas, M. V.; Polo, M. C.; Bravo, G.; Martínez-Zapater, José M.; Martínez Rodríguez, María del Carmen; Cifuentes, Alejandro
2006-01-31

Digital.CSIC (Spain)

113

Collisional and photoinitiated reaction dynamics in the ground electronic state of Ca–HCl

Sanz, Cristina ; Avoird, Ad van der ; Roncero, Octavio

11 pages, 14 figures, 1 table.-- PACS nrs.: 82.30.Cf; 82.50.-m; 82.20.Kh; 82.30.Fi; 82.20.Rp; 82.20.Fd; 33.20.Ea; 33.15.Ry; 33.15.Dj; 82.30.Hk. | Ca+HCl(ν,j) reactive collisions were studied for different rovibrational states of the HCl reactant using wave-packet calculations in reactant Jacobi coor...

DRIVER (Spanish)

116

Chloroplast DNA diversity in Prunus and its implication on genetic relationships

Bouhadida, Mariem; Martín, Juan Pedro; Eremin, G.; Pinochet, Jorge; Moreno Sánchez, María Ángeles; Gogorcena Aoiz, Yolanda
2007-01-01

Digital.CSIC (Spain)

118

Characterization of the CaENG1 gene encoding an endo-1,3-β-glucanase involved in cell separation in Candida albicans

Esteban, Pedro Felipe; Ríos, Inmaculada; García, Raúl; Dueñas, Encarnación; Plá, Jesús; Sánchez-Pérez, Miguel; Vázquez de Aldana, Carlos R.; Rey Iglesias, Francisco del
2005-11-16

Digital.CSIC (Spain)

119

Centenario de la doctrina de la coagulación sanguínea/ The centennial of blood coagulation doctrine

Izaguirre Ávila, Raúl
2005-09-01

Resumen en español El misterio de la coagulación de la sangre estimuló durante siglos la curiosidad de los investigadores. El conocimiento sobre este fenómeno se ha incrementado notablemente en el último siglo y ha permitido comprender numerosos fenómenos fisiopatogénicos en diversas enfermedades hemorrágicas y trombóticas. Con ello ha sido posible la aparición de variados medicamentos de probada eficacia para prevenirlas y tratarlas. Esta era se inició en 1905, cuando Paul Morawi (mas) tz publicó una extensa monografía sobre los cuatro factores de coagulación conocidos hasta entonces (fibrinógeno, trombina, trombokinasa y calcio). En ese trabajo propuso un modelo de coagulación dividido en dos etapas: la generación de trombina y la coagulación del fibrinógeno. A partir de la década de 1940 se vivió una verdadera época de oro de la coagulación, cuando aparecieron los frutos de la prueba de laboratorio de coagulación más empleada desde entonces, el tiempo de protrombina descrito por Armand Quick en 1935; éstos incluyeron el descubrimiento y clasificación de numerosos factores involucrados en esta función. El descubrimiento y la introducción de la heparina y los cumarínicos en la terapéutica anticoagulante abrió un panorama inconmensurable para el advenimiento de los nuevos medicamentos antitrombóticos. A mediados del siglo XX se había logrado descifrar el mecanismo de la coagulación prácticamente en forma completa y se propusieron diversos modelos basados en funciones enzimáticas secuenciales, llamadas en cadena primero y en cascada después. En la segunda mitad del siglo XX se identificaron numerosos mecanismos de regulación de la coagulación y aparecieron diversas pruebas de laboratorio que han permitido diagnosticar con alta precisión numerosas enfermedades. Las técnicas de separación sanguínea permitieron producir concentrados de factores para uso clínico. Con la identificación de los genes que codifican la síntesis de los factores de coagulación se ha hecho posible su producción portécnicas de biología molecular, siendo los de mayor trascendencia clínica la de los factores VIII, IX y VII. El modelo actual de la coagulación se basa en la activación por el factor tisular y en la participación de las células, conceptos que ya estaban implícitos en la teoría clásica de Morawitz Resumen en inglés For centuries, the mystery surrounding blood coagulation stimulated the curiosity of researchers. The knowledge about this function has increased notably in the last century and has permitted to understand numerous physiopatho logical phenomena in several hemorrhagic and thrombotic diseases, and has made it possible to develop diverse drugs of proved efficacy for prevention and therapy. All this was initiated in 1905, when Paul Morawitz published an extensive monograph on (mas) the four factors of blood coagulation known until then (fibrinogen, thrombin, thrombokinase, and calcium). In that work, he proposed a blood coagulation model in two stages: thrombin generation and fibrinogen coagulation. In the 1940s a true golden age in coagulation was started with the results of Quick's prothrombin time test, described in 1936, and since then the most employed coagulation test. Besides numerous factors involved in this function were discovered and classified. The discovery and introduction of heparin and coumarin in the anticoagulant therapeutics opened a measureless panorama for the arrival of new antithrombotic drugs. By the middle of the XX century, the mechanism of coagulation had been practically deciphered and diverse models based on sequential enzymatic function were proposed, called first chain reactions and in cascade reactions later. In the second half of the XX century, numerous regulatory mechanisms of coagulation were identified and diverse laboratory tests appeared that have allowed highly precise diagnoses of a variety of diseases. Blood separation techniques have permitted to produce factor concentrates for clinical use. With the identification of the genes that encode the synthesis of coagulation factors, it has been possible to produce them by means of molecular biology techniques, being the most significant the production of factors VIII, IX, and VII. The present model of coagulation is based on tissular factor activation and the participation of cells, concepts that were already implicit in the classical theory of Morawitz

Scientific Electronic Library Online (Spanish)

120

Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres/ Search for the spirochete Borrelia burgdorferi senso lato by polymerase chain reaction in Chilean ticks

Osorio A, Gonzalo
2001-03-01

Resumen en inglés Background: Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection. Aim: To detect the presence of B burgdorferi in Chilean ticks. Material and methods: A total o (mas) f 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions. Results: B burgdorferi was not detected in the studied ticks. Conclusions: Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative. (Rev Méd Chile 2001; 129: 270-6)

Scientific Electronic Library Online (Spanish)

121

Association study of genetic variants of pro-inflammatory chemokine and cytokine genes in systemic lupus erythematosus

Sánchez, Elena; Sabio, José M.; Callejas Rubio, José L.; Ramón, Enrique de; Garcia-Portales, Rosa; García-Hernández, Francisco J.; Jiménez-Alonso, Juan; González-Escribano, María Francisca; Martín Ibáñez, Javier; Koeleman, B.P.C.
2006-05-23

Digital.CSIC (Spain)

123

Análisis de paternidad utilizando cuatro marcadores de DNA amplificados mediante la reacción en cadena de polimerasa/ Paternity analysis using four DNA markers amplified by polymerase chain reaction

Jorquera G, Hugo; Acuña P, Mónica; Cifuentes O, Lucía; Aguirre M, Eugenia; Moreno Ch, Fabián
1999-09-01

Resumen en inglés Background: DNA typing in forensic analysis is a useful tool to analyze paternity due to its high discrimination power. Aim: To report the experience of Servicio Medico Legal in Santiago, resolving cases of dubious paternity. Subjects and methods: Four highly polymorphic loci, amplified by polymerase chain reactions, were analyzed in 153 cases of uncertain paternity. The paternity index was calculated for each case. Results: The four genetic markers analyzed provided an e (mas) xclusion probability of 0.933 for the general population in Santiago. Thirty seven cases were excluded as parents. In 31 cases, the paternity index ranged from 19 to 100, considered as probable paternity and 77 cases had an index of over 100, considered as almost certain paternity. Eight cases had an index between 0.5 and 19, considered as inconclusive. All loci met Hardy-Weinberg expectations and their frequencies were similar to other data from people living in Santiago. Conclusions: The use of these genetic markers proved to be very useful, reliable and with a high exclusion power for paternity analysis

Scientific Electronic Library Online (Spanish)

124

Analysis of LMP and TAP polymorphisms by polymerase chain reaction-restriction fragment length polymorphism in rheumatoid arthritis

Vinasco, J.; Fraile, A.; Nieto, A.; Beraun, Y.; Pareja, E.; Mataran, L.; Martín Ibáñez, Javier
1998-01-01

Digital.CSIC (Spain)

125

An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain

Gilardi-Hebenstreit, Pascale; Nieto, M. Ángela; Frain, M.; Mattéi, Marie-Geneviève; Chestier, A.; Wilkinson, David G.; Charnay, Patrick
1992-01-01

Digital.CSIC (Spain)

127

Acquired macrolide resistance in the human intestinal strain Lactobacillus rhamnosus E41 associated with a transition mutation in 23S rRNA genes

Flórez García, Ana Belén; Ladero Losada, Victor Manuel; Álvarez Martín, Pablo; Ammor, Mohammed Salim; Álvarez González, Miguel Ángel; Mayo Pérez, Baltasar
2007-07-20

Digital.CSIC (Spain)

128

A noninvasive test of exposition to toxicants: quantitative analysis of cytochrome P4501A expression in fish scales

Quirós, Laia; Raldúa, Demetrio; Navarro Cuenca, Anna; Casado Belloso, Marta; Barceló, Damià; Piña, Benjamín
2007-10-01

Digital.CSIC (Spain)

130

A convenient synthesis of C-22 and C-25 stereoisomers of cephalostatin north 1 side chain from spirostan sapogenins

Betancor, Carmen; Freire, Raimundo; Pérez-Martín, Inés; Prangé, Thierry; Suárez, Ernesto
2002-03-16

Digital.CSIC (Spain)