Sample records for SISTEMAS DE FLUJO DE CELULAS (cell flow systems)
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Sample records 1 - 20 shown. Select sample records:



1

Vertical biogenic particle flux in a western Galician ria (NW Iberian Peninsula)

Varela, Manuel; Prego Reboredo, Ricardo Francisco J.; Pazos, Yolanda
2004-03-25

Digital.CSIC (Spain)

4

The PHA test reflects acquired T-cell mediated immunocompetence in birds

Tella, José Luis; Lemus, Jesús Angel; Carrete, Martina; Blanco, Guillermo
2008-09-29

Digital.CSIC (Spain)

5
6

Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral/ Innate immunity restoration in patients with HIV/AIDS infection associated with antiretroviral therapy

Afani S, Alejandro; Jiusán L, Lorena; Raby A, Pablo; Sitia, Giovanni; Puente P, Javier; Sepúlveda C, Cecilia; Miranda W, Dante; Cabrera C, Roy; Guidotti, Luca; Lanza, Paola
2006-06-01

Resumen en inglés Background: Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. Aim: To study prospectively the restoration of the innate immune system in patients with HIV/AIDS infection during their first year on HAART. Patients and Methods: 25 naive HIV/AIDS patients, fro (mas) m San José Hospital and University of Chile Clinical Hospital, Santiago, Chile, were studied between years 2002-2003. Every 4 months after HAART initiation, CD3+, CD4+, CD8+ T lymphocytes and CD16/56+ natural killer (NK) cells were quantified by flow cytometry. NK cell cytotoxicity was measured using radioactive chrome liberation (Cr51). Tumor necrosis factor alpha (TNF-a) and interleukin-10 (IL-10) were measured in peripheral blood mononuclear cells and viral load was determined using Amplicor HIV-1 from Roche Diagnostics Systems. Results: Thirteen of the 25 patients continued in the study. They were all males, average age 35 years old (23-50). At baseline average CD4+ count was 146 cells/µL (31-362) and average viral load was 82.000 copies/mL (4.000-290.000). A raise in CD3+, CD4+, CD8+, and CD16/56 cells was noted at months 9-12 of therapy. Viral load became undetectable in the same period. NK cell function was decreased at the beginning of the therapy (1-4 months), reaching its highest values at months 9-12. There was no significant change in IL-10. TNF-a increased in six patients during the study. Conclusions: In this group of patients, innate immunity was restored during HAART. These results should be confirmed in studies with a longer follow up period and also measuring cytokines such as MIP-1a, MIP-1ß and RANTES

Scientific Electronic Library Online (Spanish)

7

Performance improvement of a PEMFC system controlling the cathode outlet air flow

Feroldi, Diego; Serra Prat, María; Riera Colomer, Jordi
2007-01-01

Digital.CSIC (Spain)

8

Numerical model for polymer electrolyte membrane fuel cells with experimental application and validation

Alonso Mora, Javier; Husar, Attila Peter; Serra Prat, María; Riera Colomer, Jordi
2009-01-01

Digital.CSIC (Spain)

9

Multi-analyte SPR immunoassays for environmental biosensing of pesticides

Mauriz, Elba; Calle Martín, Ana; Manclús, J. J.; Montoya, Ángel; Lechuga, Laura M.
2006-10-25

Digital.CSIC (Spain)

10

Motion processing with wide-field neurons in the retino-tecto-rotundal pathway

Dellen, Babette; Wessel, Ralf; Clark, John W.; Wörgötter, Florentin
2010-01-01

Digital.CSIC (Spain)

11

Modeling of a three-stage low temperature ethanol steam reforming reactor for fuel cell applications

García Mariel, Vanesa; Llorca, Jordi; Serra Prat, María; Riera Colomer, Jordi
2008-01-01

Digital.CSIC (Spain)

13

Linear identification and model adjustment of a PEM fuel cell stack

Kunusch, Cristian; Husar, Attila Peter; Puleston, Pablo Federico; Mayosky, Miguel Ángel; Moré, J.J.
2008-01-01

Digital.CSIC (Spain)

14

Improved bacteria detection by coupling magneto-immunocapture and amperometry at flow-channel microband electrodes

Laczka, Olivier; Maesa, José María; Godino, Neus; del Campo, Javier; Fougt-Hansen, Mikkel; Kutter, Jörg P.; Snakenborg, Detlef; Muñoz-Pascual, Francesc-Xavier; Baldrich, Eva
2011-02-23

Digital.CSIC (Spain)

16

Fluorescence analysis of the interaction of two peptide sequences of hepatitis GB virus C with liposomes

Muñoz, M.; Rojo, Nuria; Girona, V.; Mestres, Concepció; Busquets, M. Antònia
2003-03-15

Digital.CSIC (Spain)

17

Factors influencing the quality of a surface water supply system: The Ter River, Northeastern Spain

Fernandez-Turiel, J. L.; Gimeno, D.; Rodríguez, J. J.; Carnicero, M.; Valero, F.
2003-01-01

Digital.CSIC (Spain)

19

Embryonic nodal flow and the dynamics of nodal vesicular parcels

H. E. Cartwright, Julyan; Piro, Nicolás; Piro, Oreste; Tuval, Idan
2007-02-01

Digital.CSIC (Spain)

21

Efecto del lipopolisacárido en cultivos de células dendríticas humanas y su inhibición por la polimixina B/ Effect of lipopolysaccharides on human dentritic cell cultures and its inhibition by polymyxin B

Cuéllar, Adriana; Fonseca, Ángela; Gómez, Alberto
2004-12-01

Resumen en español Algunos estímulos de maduración utilizados en cultivos de células dendríticas, como las proteínas recombinantes obtenidas en sistemas bacterianos o los extractos proteicos de ectoparásitos, contienen lipopolisacáridos (LPS) y su presencia afecta el comportamiento de las células dendríticas en cultivo. En este trabajo se evaluó el efecto del LPS en un sistema de cultivo de células dendríticas humanas y la actividad de la polimixina B como inhibidor del efecto d (mas) el LPS. Para esto, los monocitos obtenidos a partir de sangre periférica humana se diferenciaron a células dendríticas con IL-4 y GM-CSF y como estímulo de maduración se utilizó LPS o PGE2/FNTá. La expresión de marcadores y la secreción de citocinas se evaluaron por citometría de flujo. Los resultados mostraron que la preexposición a endotoxina disminuyó la expresión de CD83, inhibió la secreción de IL-12p70, FNTá e IL-10 y disminuyó la secreción de IL-6 en células dendríticas. Además, la utilización de 10 mg/ml de polimixina B fue efectiva en la inhibición de la actividad de 1 mg/ml de LPS y la máxima concentración de polimixina B que no afectó la morfología de las células fue de 50 mg/ml. En conclusión, la polimixina B es efectiva para inhibir la actividad del LPS en los cultivos de células dendríticas. Resumen en inglés Dendritic cells have been described as effective antigen presenting cells. Human dentritic cells are highly susceptible to lipopolysaccharide (LPS) tolerance, consisting of a differential deactivation state in which some cellular functions are impaired. LPS tolerance can be experimentally induced in vitro, in which the presence of LPS strongly affects the behavior of cultured dendritic cells. Recombinant proteins obtained from bacterial systems or protein extracts of ecto (mas) parasites containing LPS can be used as stimuli to enhance maturation processes in these cells. The present study evaluated the effect of LPS in human dendritic cell cultures, and the activity of polymyxin B as an inhibitor of the LPS effect. Dendritic cells were obtained from peripheral blood monocytes in the presence of IL-4 and GM-CSF, followed by exposure with LPS and PGE2/TNFá. Surface markers and cytokine levels were evaluated by flow cytometry. The dendritic cells pre-exposed to single doses of endotoxin demonstrated a reduced capacity to mature, reduced CD83 expression, inhibited secretion of IL-12, TNFá, IL-10 and diminished secretion of IL-6. Furthermore, polymyxin B at 10 mg/ml inhibits LPS activity at 1 mg/ml. The maximum polymyxin B concentration with no effect on cellular morphology was 50 mg/ml. Consequently, polymyxin B was determined to be an effective LPS inhibitor in dendritic cell cultures.

Scientific Electronic Library Online (Spanish)

22

Design and analysis of fuel cell hybrid systems oriented to automotive applications

Feroldi, Diego; Serra Prat, María; Riera Colomer, Jordi
2009-01-01

Digital.CSIC (Spain)

25

Conjugation of a Novel Apaf-1 Inhibitor to Peptide-based Cell-Membrane Transporters. Effective Methods to Improve Inhibition of Mitochondria-Mediated Apoptosis

Orzáez, Mar; Mondragón Martínez, Laura; Marzo, Isabel; Sanclimens Pérez de Rozas, Gloria; Messeguer Peypoch, Àngel; Pérez-Payá, Enrique; Vicent, María J.
2007-05-01

Digital.CSIC (Spain)

26

Clinical relevance of the severe abnormalities of the T cell compartment in septic shock patients

Monserrat, Jorge; Pablo, Raúl de; Reyes, Eduardo; Díaz, David; Barcenilla, Hugo; Rodríguez Zapata, Manuel; Hera, Antonio de la; Prieto, Alfredo; Álvarez-Mon, Melchor
2009-02-25

Digital.CSIC (Spain)

27

Abundance and distribution of picoplankton in tropical, oligotrophic Lake Kivu, eastern Africa

Sarmento, Hugo; Unrein, Fernando; Isumbisho, Mwapu; Stenuite, Stephane; Gasol, Josep M.; Descy, Jean-Pierre
2008-04-01

Digital.CSIC (Spain)