Sample records for ESCHERICHIA COLI (escherichia coli)
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6

Escherichia coli ehl1 Gene-Positive Serotype O18ac:H31 Associated with an Outbreak of Diarrhea in a Neonatal Nursery in Neuquén City, Argentina

Caldeira, Richard; Fernández Galvez, Gabriel; Chillemi, Germán; Miliwebsky, Elizabeth; Chinen, Isabel; Rivas, Marta

Between 9 October and 12 November 1996, an outbreak of bloody diarrhea occurred in the neonatal nursery ward of the Policlínico Neuquén, in Neuquén, a city in the southwestern region of Argentina. Seven patients of the intermediate care unit were affected. Isolates of Escherichia coli O18ac:H31 that...

DRIVER (German)

24

A novel IS26 structure is surrounding blaCTX-M genes in different plasmids of German clinical isolates of Escherichia coli

Cullik, Angela; Pfeifer, Yvonne; Prager, Rita; von Baum, Heike; Witte, Wolfgang

This report focuses on the molecular characterization of 22 extended-spectrum beta-lactamase (ESBL) producing E. coli isolates collected in a German university hospital, during a period of nine months in 2006. Relationship analysis of clinical isolates was done via pulsed-field gel-electrophoresis, ...

DRIVER (German)

33

Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils

Rivas, Germán; Alfonso, Carlos; González, José Manuel; Jiménez, Mercedes; Vélez, Marisela; Schuck, Peter; Minton, Allen P.

A mechanism of noncooperative (isodesmic) assembly coupled with preferential cyclization of long polymers is proposed to explain the previously posed question of how a single-stranded filament of the bacterial cell-division protein FtsZ can assemble in an apparently cooperative manner. This proposal...

DRIVER (German)

45

Adherent and Invasive Escherichia coli Is Associated with Granulomatous Colitis in Boxer Dogs

Russell, David G.; Harel, Josee; Rishniw, Mark; Berg, Douglas E.; Bruant, Guillaume; Klaessig, Suzanne; McDonough, Patrick L.

The mucosa-associated microflora is increasingly considered to play a pivotal role in the pathogenesis of inflammatory bowel disease. This study explored the possibility that an abnormal mucosal flora is involved in the etiopathogenesis of granulomatous colitis of Boxer dogs (GCB). Colonic biopsy sa...

DRIVER (German)

55

A 12-Base-Pair Deletion in the Flagellar Master Control Gene flhC Causes Nonmotility of the Pathogenic German Sorbitol-Fermenting Escherichia coli O157:H− Strains

Monday, Steven R.; Feng, Peter C. H.; Minnich, Scott A.

An atypical, Stx2-producing, pathogenic Escherichia coli O157:H− strain has been isolated with increasing frequency from hemolytic uremic syndrome patients in Germany. The lack of the H7 antigen coupled with the strain's ability to ferment sorbitol and express β-glucuronidase have complicated its de...

DRIVER (German)

58

Isolation and Characterization of Escherichia coli tolC Mutants Defective in Secreting Enzymatically Active Alpha-Hemolysin

Vakharia, Hema; Misra, Rajeev; German, Greg J.

This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these m...

DRIVER (German)

61

Increased Prevalence of Class I Integrons in Escherichia coli, Klebsiella Species, and Enterobacter Species Isolates over a 7-Year Period in a German University Hospital

Follmann, Patrick; Schmitz, Franz-Josef; Geisel, Roland; Hafner, Dieter; Kirschke, Christian; Fluit, Ad C.; Verhoef, Jan

The prevalence of integrons in five enterobacterial species was analyzed in 900 blood culture isolates from 1993, 1996, and 1999. Remarkably, the prevalence increased from 4.7% in 1993 to 9.7% in 1996 and finally to 17.4% in 1999 (P < 0.01). Within 7 years the combined percentage of P1 strong pro...

DRIVER (German)

67

Molecular Characterization of FOX-4, a New AmpC-Type Plasmid-Mediated β-Lactamase from an Escherichia coli Strain Isolated in Spain

Monzón, Carmelo; Bou, Germán; Ojeda, Mar; Oliver, Antonio; Martínez-Beltrán, Jesús

A clinical strain of Escherichia coli (Ec GCE) displayed resistance to cefoxitin, cefotetan, cefotaxime, and ceftazidime. Susceptibility was not restored by the addition of clavulanic acid. Two β-lactamases with apparent pIs of 5.4 and 6.4 were identified; the β-lactamase with a pI of 6.4 was transf...

DRIVER (German)

75

Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes.

German, A L; Nataro, J P; Maneval, D R; Deng, Y; Martin, W C; Levine, M M

Strains of enteroaggregative Escherichia coli (EAggEC) have been implicated in several studies as important agents of persistent diarrhea among infants in the developing world. We have previously shown that the aggregative adherence (AA) property of EAggEC is associated with the presence of a 60-MDa...

DRIVER (German)

90

Identification and Broad Dissemination of the CTX-M-14 β-Lactamase in Different Escherichia coli Strains in the Northwest Area of Spain

Canle, Delia; Bou, German; Tomas, Maria; Cartelle, Monica; Molina, Francisca; Guerrero, Antonio; Moure, Rita; Eiros, Jose Maria

During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 β-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype...

DRIVER (German)

98

Proteome, transcriptome and metabolome plasticity in closely related strains of Escherichia coli - K12 and its diversity during molecular evolution processes

Vijayendran, Chandran

Escherichia coli, originally known as Bacterium coli commune, was identified in 1885 by the German pediatrician, Theodor Escherich. They are Gram-negative, straight and rod-shaped bacteria from the Enterobacteriaceae family. Due to its rapid growth rate, simple nutritional requirements and establish...

DRIVER (German)

99

Inducible Gene Expression from African Swine Fever Virus Recombinants: Analysis of the Major Capsid Protein p72

Viñuela, Eladio; García-Escudero, Ramón; Almazán, Fernando; Andrés, Germán

A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding the lac repressor and a strong virus late promoter modified by the i...

DRIVER (German)

100

Incidence of the Recently Described Sulfonamide Resistance Gene sul3 among German Salmonella enterica Strains Isolated from Livestock and Food

Guerra, Beatriz; Helmuth, Reiner; Junker, Ernst

The sul3 gene recently described in Escherichia coli was found in 22 of 512 (4.3%) German Salmonella isolates from different regions and sources and of different serotypes, antimicrobial resistance phenotypes, and genomic groups. This is the first report on the prevalence of sul3 among Salmonella st...

DRIVER (German)

101

High-Level Resistance to Ceftazidime Conferred by a Novel Enzyme, CTX-M-32, Derived from CTX-M-1 through a Single Asp240-Gly Substitution

Moure, Rita; Cartelle, Monica; Molina, Francisca; del Mar Tomas, Maria; Villanueva, Rosa; Bou, German

A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (blaCTX-M-32) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed ...

DRIVER (German)

102

Molecular Cloning and Sequencing of the aroA Gene from Actinobacillus pleuropneumoniae and Its Use in a PCR Assay for Rapid Identification

Marcos, Javier Yugueros; Moral, Carmen Hernanz; Salazar, María Sánchez; Soriano, Alberto Cascón; Ramos, Susana Suárez

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5′ and 3′ termini wer...

DRIVER (German)

103

Molecular Characterization of the Aeromonas hydrophila aroA Gene and Potential Use of an Auxotrophic aroA Mutant as a Live Attenuated Vaccine

Carrasco, Germán Naharro; Cortés, Alberto Villena; Hernanz Moral, Carmen; Fierro, Pilar López; del Castillo, Emilio Flaño

The aroA gene of Aeromonas hydrophila SO2/2, encoding 5-enolpyruvylshikimate 3-phosphate synthase, was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. The nucleotide sequence of the A. hydrophila aroA gene encoded a p...

DRIVER (German)

104

A Tripeptide Deletion in the Class C beta-Lactamase FOX-4 Enzyme Impairs Cefoxitin Hydrolysis and Slightly Increases Susceptibility to beta-Lactamase Inhibitors

Mallo, Susana; Pérez-Llarena, Francisco J.; Kerff, Frédéric; Fernández-Moreira, Esteban; Soares, Nelson C.; Galleni, Moreno

OBJECTIVES: A natural variant of the AmpC enzyme from Escherichia coli HKY28 with a tripeptide deletion (Gly-286/Ser-287/Asp-288) was recently described. The isolate produced an inhibitor-sensitive AmpC beta-lactamase variant that also conferred higher than usual levels of resistance to ceftazidime ...

DRIVER (German)

105

A Tripeptide Deletion in the Class C beta-Lactamase FOX-4 Enzyme Impairs Cefoxitin Hydrolysis and Slightly Increases Susceptibility to beta-Lactamase Inhibitors

Mallo, Susana; Pérez-Llarena, Francisco J.; Kerff, Frédéric; Fernández-Moreira, Esteban; Soares, Nelson C.; Galleni, Moreno

OBJECTIVES: A natural variant of the AmpC enzyme from Escherichia coli HKY28 with a tripeptide deletion (Gly-286/Ser-287/Asp-288) was recently described. The isolate produced an inhibitor-sensitive AmpC beta-lactamase variant that also conferred higher than usual levels of resistance to ceftazidime ...

DRIVER (German)

108

Two different pathways are involved in the beta-oxidation of n-alkanoic and n-phenylalkanoic acids in Pseudomonas putida U: genetic studies and biotechnological applications

Olivera, Elías R.; Carnicero, David; García, Belén; Miñambres Rodríguez, Baltasar; Moreno, Miguel A.

12 pages, 4 figures.-- PMID: 11251808 [PubMed]. | In Pseudomonas putida U, the degradation of n-alkanoic and n-phenylalkanoic acids is carried out by two sets of beta-oxidation enzymes (betaI and betaII). Whereas the first one (called betaI) is constitutive and catalyses the degradation of n-alkanoi...

DRIVER (German)