Prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) are secreted by the prostate into seminal plasma and through leakage into blood. This leakage increases rapidly in prostate disease, and PSA is used worldwide as a marker of prostate cancer. PSA is activated in vitro by hK2, and ...
Doctoral degree 03-11-1998; Department of Chemical Engineering; Supervisors: A.L. German and C.A.M.G. Cramers
Doctoral degree 12-03-1996; Department of Chemical Engineering; Supervisors: A.L. German and C.A.M.G. Cramers; co-promotor A.M. van Herk
Determination of pesticide residues in waters from small loughs by solid-phase extraction and combined use of gas chromatography with electron-capture and nitrogen-phosphorus detection and high-performance liquid chromatography with diode array detection
Determination of chlorpyrifos in air, leaves and soil from a greenhouse by gas-chromatography with nitrogen-phosphorus detection, high-performance liquid chromatography and capillary electrophoresis (Short communication)
Determination of a variety of chemical classes of pesticides in surface and ground waters by off-line solid-phase extraction, gas chromatography with electron-capture and nitrogen-phosphorus detection, and high-performance liquid chromatography with post-column derivatization and fluorescence detection
Doctoral degree 06-01-1999 ; Department of Chemical Engineering ; Supervisors: C.A.M.G. Cramers and A.L. German
Doctoral degree 27-05-1999 ; Department of Chemical Engineering ; Supervisors: A.L. German and P.J. Schoenmakers; co-promotor B. Klumperman
The properties of a variant phosphoglycerate kinase (PGK) found in a large German clan were examined. The normal and variant enzymes, isolated by affinity chromatography, have the same molecular weight, specific activity, substrate affinity, and nearly identical pH-optima. Using immunoinactivation a...