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Sample records for musculus phr1 mycbp2

  1. A damage-responsive DNA binding protein regulates transcription of the yeast DNA repair gene PHR1

    International Nuclear Information System (INIS)

    Sebastian, J.; Sancar, G.B.

    1991-01-01

    The PHR1 gene of Saccharomyces cerevisiae encodes the DNA repair enzyme photolyase. Transcription of PHR1 increases in response to treatment of cells with 254-nm radiation and chemical agents that damage DNA. The authors here the identification of a damage-responsive DNA binding protein, termed photolyase regulatory protein (PRP), and its cognate binding site, termed the PHR1 transcription after DNA damage. PRP activity, monitored by electrophoretic-mobility-shift assay, was detected in cells during normal growth but disappeared within 30 min after irradiation. Copper-phenanthroline footprinting of PRP-DNA complexes revealed that PRP protects a 39-base-pair region of PHR1 5' flanking sequence beginning 40 base pairs upstream from the coding sequence. Thus these observations establish that PRP is a damage-responsive repressor of PHR1 transcription

  2. Molecular analysis of Phr peptide processing in Bacillus subtilis.

    Science.gov (United States)

    Stephenson, Sophie; Mueller, Christian; Jiang, Min; Perego, Marta

    2003-08-01

    In Bacillus subtilis, an export-import pathway regulates production of the Phr pentapeptide inhibitors of Rap proteins. Processing of the Phr precursor proteins into the active pentapeptide form is a key event in the initiation of sporulation and competence development. The PhrA (ARNQT) and PhrE (SRNVT) peptides inhibit the RapA and RapE phosphatases, respectively, whose activity is directed toward the Spo0F approximately P intermediate response regulator of the sporulation phosphorelay. The PhrC (ERGMT) peptide inhibits the RapC protein acting on the ComA response regulator for competence with regard to DNA transformation. The structural organization of PhrA, PhrE, and PhrC suggested a role for type I signal peptidases in the processing of the Phr preinhibitor, encoded by the phr genes, into the proinhibitor form. The proinhibitor was then postulated to be cleaved to the active pentapeptide inhibitor by an additional enzyme. In this report, we provide evidence that Phr preinhibitor proteins are subject to only one processing event at the peptide bond on the amino-terminal end of the pentapeptide. This processing event is most likely independent of type I signal peptidase activity. In vivo and in vitro analyses indicate that none of the five signal peptidases of B. subtilis (SipS, SipT, SipU, SipV, and SipW) are indispensable for Phr processing. However, we show that SipV and SipT have a previously undescribed role in sporulation, competence, and cell growth.

  3. Molecular Analysis of Phr Peptide Processing in Bacillus subtilis†

    Science.gov (United States)

    Stephenson, Sophie; Mueller, Christian; Jiang, Min; Perego, Marta

    2003-01-01

    In Bacillus subtilis, an export-import pathway regulates production of the Phr pentapeptide inhibitors of Rap proteins. Processing of the Phr precursor proteins into the active pentapeptide form is a key event in the initiation of sporulation and competence development. The PhrA (ARNQT) and PhrE (SRNVT) peptides inhibit the RapA and RapE phosphatases, respectively, whose activity is directed toward the Spo0F∼P intermediate response regulator of the sporulation phosphorelay. The PhrC (ERGMT) peptide inhibits the RapC protein acting on the ComA response regulator for competence with regard to DNA transformation. The structural organization of PhrA, PhrE, and PhrC suggested a role for type I signal peptidases in the processing of the Phr preinhibitor, encoded by the phr genes, into the proinhibitor form. The proinhibitor was then postulated to be cleaved to the active pentapeptide inhibitor by an additional enzyme. In this report, we provide evidence that Phr preinhibitor proteins are subject to only one processing event at the peptide bond on the amino-terminal end of the pentapeptide. This processing event is most likely independent of type I signal peptidase activity. In vivo and in vitro analyses indicate that none of the five signal peptidases of B. subtilis (SipS, SipT, SipU, SipV, and SipW) are indispensable for Phr processing. However, we show that SipV and SipT have a previously undescribed role in sporulation, competence, and cell growth. PMID:12897006

  4. Secure Dynamic access control scheme of PHR in cloud computing.

    Science.gov (United States)

    Chen, Tzer-Shyong; Liu, Chia-Hui; Chen, Tzer-Long; Chen, Chin-Sheng; Bau, Jian-Guo; Lin, Tzu-Ching

    2012-12-01

    With the development of information technology and medical technology, medical information has been developed from traditional paper records into electronic medical records, which have now been widely applied. The new-style medical information exchange system "personal health records (PHR)" is gradually developed. PHR is a kind of health records maintained and recorded by individuals. An ideal personal health record could integrate personal medical information from different sources and provide complete and correct personal health and medical summary through the Internet or portable media under the requirements of security and privacy. A lot of personal health records are being utilized. The patient-centered PHR information exchange system allows the public autonomously maintain and manage personal health records. Such management is convenient for storing, accessing, and sharing personal medical records. With the emergence of Cloud computing, PHR service has been transferred to storing data into Cloud servers that the resources could be flexibly utilized and the operation cost can be reduced. Nevertheless, patients would face privacy problem when storing PHR data into Cloud. Besides, it requires a secure protection scheme to encrypt the medical records of each patient for storing PHR into Cloud server. In the encryption process, it would be a challenge to achieve accurately accessing to medical records and corresponding to flexibility and efficiency. A new PHR access control scheme under Cloud computing environments is proposed in this study. With Lagrange interpolation polynomial to establish a secure and effective PHR information access scheme, it allows to accurately access to PHR with security and is suitable for enormous multi-users. Moreover, this scheme also dynamically supports multi-users in Cloud computing environments with personal privacy and offers legal authorities to access to PHR. From security and effectiveness analyses, the proposed PHR access

  5. Introduction of the yeast DNA repair gene PHR1 into normal and xeroderma pigmentosum human cells

    International Nuclear Information System (INIS)

    Whyte, D.B.

    1988-01-01

    The goal of the work described herein is to determine how UV light kills and mutates human cells. Specifically, the hypothesis to be tested states that the major cause of cell death is the cyclobutane dimer. The yeast (S. cerevisiae) enzyme photolyase provides an elegant means of dissecting the biological effects of the two lesions. Photolyase, the product of the PHR1 gene, catalyzes the visible light-dependent reversal of cyclobutane pyrimidine dimers. Introducing the gene for photolyase into human cells, which do not have a functional photoreactivation mechanism, should allow specific repair of cyclobutane pyrimidine dimers. To express the yeast DNA repair gene in human cells, the yeast PHR1 coding sequence was cloned into the mammalian expression vector pRSV4NEO-I. The resulting plasmid, pRSVPHR1, contains the coding sequence of the yeast gene, under control of transcription signals recognized by mammalian cells, and the dominant selectable gene neo. pRSVPHR1 was introduced into normal and XP SV40-transformed fibroblasts by the calcium phosphate coprecipitation technique, and G418-resistant clones were isolated. The level of PHR1 expression was determined by cytoplasmic RNA dot blots. Two clones, XP-3B and GM-20A, had high levels of expression

  6. Structural Basis of Rap Phosphatase Inhibition by Phr Peptides

    Science.gov (United States)

    Gallego del Sol, Francisca; Marina, Alberto

    2013-01-01

    Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR), are the main signal transduction devices in bacteria. In Bacillus, the Rap protein family modulates complex signaling processes mediated by two-component systems, such as competence, sporulation, or biofilm formation, by inhibiting the RR components involved in these pathways. Despite the high degree of sequence homology, Rap proteins exert their activity by two completely different mechanisms of action: inducing RR dephosphorylation or blocking RR binding to its target promoter. However the regulatory mechanism involving Rap proteins is even more complex since Rap activity is antagonized by specific signaling peptides (Phr) through a mechanism that remains unknown at the molecular level. Using X-ray analyses, we determined the structure of RapF, the anti-activator of competence RR ComA, alone and in complex with its regulatory peptide PhrF. The structural and functional data presented herein reveal that peptide PhrF blocks the RapF-ComA interaction through an allosteric mechanism. PhrF accommodates in the C-terminal tetratricopeptide repeat domain of RapF by inducing its constriction, a conformational change propagated by a pronounced rotation to the N-terminal ComA-binding domain. This movement partially disrupts the ComA binding site by triggering the ComA disassociation, whose interaction with RapF is also sterically impaired in the PhrF-induced conformation of RapF. Sequence analyses of the Rap proteins, guided by the RapF-PhrF structure, unveil the molecular basis of Phr recognition and discrimination, allowing us to relax the Phr specificity of RapF by a single residue change. PMID:23526880

  7. The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells

    Directory of Open Access Journals (Sweden)

    Laura Popolo

    2017-10-01

    Full Text Available Candida albicans is an opportunistic microorganism that can become a pathogen causing mild superficial mycosis or more severe invasive infections that can be life-threatening for debilitated patients. In the etiology of invasive infections, key factors are the adaptability of C. albicans to the different niches of the human body and the transition from a yeast form to hypha. Hyphal morphology confers high adhesiveness to the host cells, as well as the ability to penetrate into organs. The cell wall plays a crucial role in the morphological changes C. albicans undergoes in response to specific environmental cues. Among the different categories of enzymes involved in the formation of the fungal cell wall, the GH72 family of transglycosylases plays an important assembly role. These enzymes cut and religate β-(1,3-glucan, the major determinant of cell shape. In C. albicans, the PHR family encodes GH72 enzymes, some of which work in specific environmental conditions. In this review, we will summarize the work from the initial discovery of PHR genes to the study of the pH-dependent expression of PHR1 and PHR2, from the characterization of the gene products to the recent findings concerning the stress response generated by the lack of GH72 activity in C. albicans hyphae.

  8. The use of biometrics in the Personal Health Record (PHR).

    Science.gov (United States)

    Bonney, Wilfred

    2011-01-01

    The emergence of the Personal Health Record (PHR) has made individual health information more readily accessible to a wide range of users including patients, consumers, practitioners, and healthcare providers. However, increased accessibility of PHR threatens the confidentiality, privacy, and security of personalized health information. Therefore, a need for robust and reliable forms of authentication is of prime concern. The concept of biometric authentication is now highly visible to healthcare providers as a technology to prevent unauthorized access to individual health information. Implementing biometric authentication mechanisms to protect PHR facilitates access control and secure exchange of health information. In this paper, a literature review is used to explore the key benefits, technical barriers, challenges, and ethical implications for using biometric authentication in PHR.

  9. Resource management in Diffserv measurement-based admission control PHR

    NARCIS (Netherlands)

    Westberg, L.; Heijenk, Geert; Karagiannis, Georgios; Oosthoek, S.; Partain, D.; Rexhepi, Vlora; Szabo, R.; Wallentin, P.; El Allali, H.

    2002-01-01

    The purpose of this draft is to present the Resource Management in Diffserv (RMD) Measurement-Based Admission Control (RIMA) Per Hop Reservation (PHR) protocol. The RIMA PHR protocol is used on a per-hop basis in a Differentiated Services (Diffserv) domain and extends the Diffserv Per Hop Behavior

  10. Resource Management in Diffserv On DemAnd (RODA) PHR

    NARCIS (Netherlands)

    Westberg, L.; Jacobsson, M.; de Kogel, M.; Oosthoek, S.; Partain, D.; Rexhepi, V.; Wallentin, P.; Karagiannis, Georgios

    The purpose of this draft is to present the Resource Management in Diffserv (RMD) On DemAnd (RODA) Per Hop Reservation (PHR) protocol. The RODA PHR protocol is used on a per-hop basis in a Differentiated Services (Diffserv) domain and extends the Diffserv Per Hop Behavior (PHB) with resource

  11. Characterization of the photolyase-like iron sulfur protein PhrB from Agrobacterium tumefaciens by Mössbauer spectroscopy

    Science.gov (United States)

    Bauer, T. O.; Graf, D.; Lamparter, T.; Schünemann, V.

    2014-04-01

    High field Mössbauer spectroscopy has been used to characterize the [4Fe-4S] 2 +cluster of the protein PhrB from Agrobacterium tumefaciens which belongs to the cryptochrome/photolyase family (CPF) and which biological function has previously been shown to be DNA repair. Mössbauer spectra taken of the as prepared protein reveal δ = 0. 42 mms - 1, and Δ E Q = 1. 26 mms - 1as well as an asymmetry parameter of η = 0. 8. These parameters are characteristic for a ferredoxin-type [4Fe-4S] 2 +cluster. In order to investigate whether this cluster is involved in DNA-repair the protein has also been studied in its photoactivated state during DNA binding. The so obtained data sets exhibit essentially the same Mössbauer parameters as those of the non-activated PhrB. This indicates that during DNA repair the [4Fe-4S] 2 +cluster of PhrB has no significant amounts of transition states which have conformational changes compared to the resting state of the protein and which have life times of several seconds or longer.

  12. The value of personal health record (PHR) systems.

    Science.gov (United States)

    Kaelber, David; Pan, Eric C

    2008-11-06

    Personal health records (PHRs) are a rapidly growing area of health information technology despite a lack of significant value-based assessment.Here we present an assessment of the potential value of PHR systems, looking at both costs and benefits.We examine provider-tethered, payer-tethered, and third-party PHRs, as well as idealized interoperable PHRs. An analytical model was developed that considered eight PHR application and infrastructure functions. Our analysis projects the initial and annual costs and annual benefits of PHRs to the entire US over the next 10 years.This PHR analysis shows that all forms of PHRs have initial net negative value. However, at the end of 10 years, steady state annual net value ranging from$13 billion to -$29 billion. Interoperable PHRs provide the most value, followed by third-party PHRs and payer-tethered PHRs also showing positive net value. Provider-tethered PHRs constantly demonstrating negative net value.

  13. Identification and field evaluation of attractants for the cranberry weevil, Anthonomus musculus Say.

    Science.gov (United States)

    Szendrei, Zsofia; Averill, Anne; Alborn, Hans; Rodriguez-Saona, Cesar

    2011-04-01

    Studies were conducted to develop an attractant for the cranberry weevil, Anthonomus musculus, a pest of blueberry and cranberry flower buds and flowers in the northeastern United States. In previous studies, we showed that cinnamyl alcohol, the most abundant blueberry floral volatile, and the green leaf volatiles (Z)-3-hexenyl acetate and hexyl acetate, emitted from both flowers and flower buds, elicit strong antennal responses from A. musculus. Here, we found that cinnamyl alcohol did not increase capture of A. musculus adults on yellow sticky traps compared with unbaited controls; however, weevils were highly attracted to traps baited with the Anthonomus eugenii Cano aggregation pheromone, indicating that these congeners share common pheromone components. To identify the A. musculus aggregation pheromone, headspace volatiles were collected from adults feeding on blueberry or cranberry flower buds and analyzed by gas chromatography-mass spectrometry. Three male-specific compounds were identified: (Z)-2-(3,3-dimethyl-cyclohexylidene) ethanol (Z grandlure II); (Z)-(3,3-dimethylcyclohexylidene) acetaldehyde (grandlure III); and (E)-(3,3- dimethylcyclohexylidene) acetaldehyde (grandlure IV). A fourth component, (E)-3,7-dimethyl-2,6-octadien-1-ol (geraniol), was emitted in similar quantities by males and females. The emission rates of these volatiles were about 2.8, 1.8, 1.3, and 0.9 ng/adult/d, respectively. Field experiments in highbush blueberry (New Jersey) and cranberry (Massachusetts) examined the attraction of A. musculus to traps baited with the male-produced compounds and geraniol presented alone and combined with (Z)-3-hexenyl acetate and hexyl acetate, and to traps baited with the pheromones of A. eugenii and A. grandis. In both states and crops, traps baited with the A. musculus male-produced compounds attracted the highest number of adults. Addition of the green leaf volatiles did not affect A. musculus attraction to its pheromone but skewed the sex ratio

  14. Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.

    Science.gov (United States)

    Bongiorni, Cristina; Ishikawa, Shu; Stephenson, Sophie; Ogasawara, Naotake; Perego, Marta

    2005-07-01

    The 11 Rap proteins of Bacillus subtilis comprise a conserved family of tetratricopeptide (TPR)-containing regulatory proteins. Their activity is inhibited by specific Phr pentapeptides produced from the product of phr genes through an export-import maturation process. We found that one of the proteins, namely RapF, is involved in the regulation of competence to DNA transformation. The ComA response regulator and transcription factor for initiation of competence development is the target of RapF. Specific binding of RapF to the carboxy-terminal DNA-binding domain of ComA inhibits the response regulator's ability to bind its target DNA promoters. The PhrF C-terminal pentapeptide, QRGMI, inhibits RapF activity. The activity of RapF and PhrF in regulating competence development is analogous to the previously described activity of RapC and PhrC (L. J. Core and M. Perego, Mol. Microbiol. 49:1509-1522, 2003). In fact, the RapF and PhrF pair of proteins acts synergistically with RapC and PhrC in the overall regulation of the ComA transcription factor. Since the transcription of the RapC- and RapF-encoding genes is positively regulated by their own target ComA, an autoregulatory circuit must exist for the competence transcription factor in order to modulate its activity.

  15. Synergistic Regulation of Competence Development in Bacillus subtilis by Two Rap-Phr Systems† ‡

    Science.gov (United States)

    Bongiorni, Cristina; Ishikawa, Shu; Stephenson, Sophie; Ogasawara, Naotake; Perego, Marta

    2005-01-01

    The 11 Rap proteins of Bacillus subtilis comprise a conserved family of tetratricopeptide (TPR)-containing regulatory proteins. Their activity is inhibited by specific Phr pentapeptides produced from the product of phr genes through an export-import maturation process. We found that one of the proteins, namely RapF, is involved in the regulation of competence to DNA transformation. The ComA response regulator and transcription factor for initiation of competence development is the target of RapF. Specific binding of RapF to the carboxy-terminal DNA-binding domain of ComA inhibits the response regulator's ability to bind its target DNA promoters. The PhrF C-terminal pentapeptide, QRGMI, inhibits RapF activity. The activity of RapF and PhrF in regulating competence development is analogous to the previously described activity of RapC and PhrC (L. J. Core and M. Perego, Mol. Microbiol. 49:1509-1522, 2003). In fact, the RapF and PhrF pair of proteins acts synergistically with RapC and PhrC in the overall regulation of the ComA transcription factor. Since the transcription of the RapC- and RapF-encoding genes is positively regulated by their own target ComA, an autoregulatory circuit must exist for the competence transcription factor in order to modulate its activity. PMID:15968044

  16. Machine Learning for Knowledge Extraction from PHR Big Data.

    Science.gov (United States)

    Poulymenopoulou, Michaela; Malamateniou, Flora; Vassilacopoulos, George

    2014-01-01

    Cloud computing, Internet of things (IOT) and NoSQL database technologies can support a new generation of cloud-based PHR services that contain heterogeneous (unstructured, semi-structured and structured) patient data (health, social and lifestyle) from various sources, including automatically transmitted data from Internet connected devices of patient living space (e.g. medical devices connected to patients at home care). The patient data stored in such PHR systems constitute big data whose analysis with the use of appropriate machine learning algorithms is expected to improve diagnosis and treatment accuracy, to cut healthcare costs and, hence, to improve the overall quality and efficiency of healthcare provided. This paper describes a health data analytics engine which uses machine learning algorithms for analyzing cloud based PHR big health data towards knowledge extraction to support better healthcare delivery as regards disease diagnosis and prognosis. This engine comprises of the data preparation, the model generation and the data analysis modules and runs on the cloud taking advantage from the map/reduce paradigm provided by Apache Hadoop.

  17. Applicability of IHE/Continua components for PHR systems: learning from experiences.

    Science.gov (United States)

    Urbauer, Philipp; Sauermann, Stefan; Frohner, Matthias; Forjan, Mathias; Pohn, Birgit; Mense, Alexander

    2015-04-01

    Capturing personal health data using smartphones, PCs or other devices, and the reuse of the data in personal health records (PHR) is becoming more and more attractive for modern health-conscious populations. This paper analyses interoperability specifications targeting standards-based communication of computer systems and personal health devices (e.g. blood pressure monitor) in healthcare from initiatives like Integrating the Healthcare Enterprise (IHE) and Continua Health Alliance driven by industry and healthcare professionals. Furthermore it identifies certain contradictions and gaps in the specifications and suggests possible solutions. Despite these shortcomings, the specifications allow fully functional implementations of PHR systems. Henceforth, both big business and small and medium-sized enterprises (SMEs) can actively contribute to the widespread use of large-scale interoperable PHR systems. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Security challenges in integration of a PHR-S into a standards based national EHR.

    Science.gov (United States)

    Mense, Alexander; Hoheiser Pförtner, Franz; Sauermann, Stefan

    2014-01-01

    Health related data provided by patients themselves is expected to play a major role in future healthcare. Data from personal health devices, vaccination records, health diaries or observations of daily living, for instance, is stored in personal health records (PHR) which are maintained by personal health record systems (PHR-S). Combining this information with medical records provided by healthcare providers in electronic health records (EHR) is one of the next steps towards "personal care". Austria currently sets up a nationwide EHR system that incorporates all healthcare providers and is technically based on international standards (IHE, HL7, OASIS, ...). Looking at the expected potential of merging PHR and EHR data it is worth to analyse integration approaches. Although knowing that an integration requires the coordination of processes, information models and technical architectures, this paper specifically focuses on security issues by evaluating general security requirements for a PHR-S (based on HL7 PHR-S FM), comparing them with the information security specifications for the Austrian's national EHR (based on ISO/IES 27000 series) and identifying the main challenges as well as possible approaches.

  19. Factors Affecting Usage of a Personal Health Record (PHR) to Manage Health

    Science.gov (United States)

    Taha, Jessica; Czaja, Sara J.; Sharit, Joseph; Morrow, Daniel G.

    2018-01-01

    As the health care industry shifts into the digital age, patients are increasingly being provided with access to electronic personal health records (PHRs) that are tethered to their provider-maintained electronic health records. This unprecedented access to personal health information can enable patients to more effectively manage their health, but little is actually known about patients’ ability to successfully use a PHR to perform health management tasks or the individual factors that influence task performance. This study evaluated the ability of 56 middle-aged adults (40–59 years) and 51 older adults (60–85 years) to use a simulated PHR to perform 15 common health management tasks encompassing medication management, review/interpretation of lab/test results, and health maintenance activities. Results indicated that participants in both age groups experienced significant difficulties in using the PHR to complete routine health management tasks. Data also showed that older adults, particularly those with lower numeracy and technology experience, encountered greater problems using the system. Furthermore, data revealed that the cognitive abilities predicting one’s task performance varied according to the complexity of the task. Results from this study identify important factors to consider in the design of PHRs so that they meet the needs of middle-aged and older adults. As deployment of PHRs is on the rise, knowledge of the individual factors that impact effective PHR use is critical to preventing an increase in health care disparities between those who are able to use a PHR and those who are not. PMID:24364414

  20. Teratogenic effect of yogurt in mice fetus (Mus musculus)

    OpenAIRE

    Dwisari Dillasamola; Almahdy A; Amirah Desri; Skunda Diliarosta

    2018-01-01

    Yogurt is one of the dairy products made from lactic acid fermentation by using Lactobacillus bulgaricus and Streptococcus thermophilus. A study on teratogenic effects of yogurt on the white female mice fetus (Mus musculus) has been carried out. Pregnant mice used were 20 which divided into 4 groups : the control group, D1, D2, and D3. The treatments giveThe mice were Distidelled water (control), 0.52 yogurt (D1), 1.04  yogurt (D2), and 2.08 g yogurt (D3). Data were analyzed using one-way ANO...

  1. OmniPHR: A distributed architecture model to integrate personal health records.

    Science.gov (United States)

    Roehrs, Alex; da Costa, Cristiano André; da Rosa Righi, Rodrigo

    2017-07-01

    The advances in the Information and Communications Technology (ICT) brought many benefits to the healthcare area, specially to digital storage of patients' health records. However, it is still a challenge to have a unified viewpoint of patients' health history, because typically health data is scattered among different health organizations. Furthermore, there are several standards for these records, some of them open and others proprietary. Usually health records are stored in databases within health organizations and rarely have external access. This situation applies mainly to cases where patients' data are maintained by healthcare providers, known as EHRs (Electronic Health Records). In case of PHRs (Personal Health Records), in which patients by definition can manage their health records, they usually have no control over their data stored in healthcare providers' databases. Thereby, we envision two main challenges regarding PHR context: first, how patients could have a unified view of their scattered health records, and second, how healthcare providers can access up-to-date data regarding their patients, even though changes occurred elsewhere. For addressing these issues, this work proposes a model named OmniPHR, a distributed model to integrate PHRs, for patients and healthcare providers use. The scientific contribution is to propose an architecture model to support a distributed PHR, where patients can maintain their health history in an unified viewpoint, from any device anywhere. Likewise, for healthcare providers, the possibility of having their patients data interconnected among health organizations. The evaluation demonstrates the feasibility of the model in maintaining health records distributed in an architecture model that promotes a unified view of PHR with elasticity and scalability of the solution. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Meiotic synapsis of homogeneously staining regions (HSRs) in chromosome 1 of Mus musculus.

    Science.gov (United States)

    Winking, H; Reuter, C; Traut, W

    1993-05-01

    About 50 copies of a long-range repeat DNA family with a repeat size of roughly 100 kb and with sequence homology to mRNAs are clustered in the G-light band D of chromosome 1 of the house mouse, Mus musculus. We studied amplified versions of the cluster which are found in many wild populations of M. musculus. They are cytogenetically conspicuous as one or two C-band positive homogeneously staining regions (single- and double band HSRs) which increase the mitotic length of chromosome 1. The double band HSR was phylogenetically derived from a single band HSR by a paracentric inversion. In homozygous condition, such HSRs contribute, albeit not as much as expected from their mitotic length, to the synaptonemal complex (SC) length of chromosome 1. In HSR heterozygous animals an elongation of the SCs was not noticeable. In single band HSR heterozygous males, synapsis proceeds regularly and continuously from the distal telomere towards the centromeric end without forming buckles. Thus, the single band HSR has no adverse effect on pairing. The same straight pairing behaviour was found in the majority of double band HSR heterozygous spermatocytes. This shows that extensive nonhomologous pairing can take place in the earliest phase of synapsis. Synapsis was discontinuous, leaving the central part of the bivalent 1 asynapsed, in only 14.3% of double band HSR heterozygous cells. In such cells the chromosome 1 SC is completed at a later stage of meiosis. The delay is presumably an effect of the inversion that includes one HSR band and the segment between the two HSR bands.

  3. Host subspecific viral strains in European house mice: Murine cytomegalovirus in the Eastern (Mus musculus musculus) and Western house mouse (Mus musculus domesticus).

    Science.gov (United States)

    Čížková, Dagmar; Baird, Stuart J E; Těšíková, Jana; Voigt, Sebastian; Ľudovít, Ďureje; Piálek, Jaroslav; Goüy de Bellocq, Joëlle

    2018-06-09

    Murine cytomegalovirus (MCMV) has been reported from house mice (Mus musculus) worldwide, but only recently from Eastern house mice (M. m. musculus), of particular interest because they form a semi-permeable species barrier in Europe with Western house mice, M. m. domesticus. Here we report genome sequences of EastMCMV (from Eastern mice), and set these in the context of MCMV genomes from genus Mus hosts. We show EastMCMV and WestMCMV are genetically distinct. Phylogeny splitting analyses show a genome wide (94%) pattern consistent with no West-East introgression, the major exception (3.8%) being a genome-terminal region of duplicated genes involved in host immune system evasion. As expected from its function, this is a region of maintenance of ancestral polymorphism: The lack of clear splitting signal cannot be interpreted as evidence of introgression. The EastMCMV genome sequences reported here can therefore serve as a well-described resource for exploration of murid MCMV diversity. Copyright © 2018 Elsevier Inc. All rights reserved.

  4. A Framework for Privacy-preserving Classification of Next-generation PHR data.

    Science.gov (United States)

    Koufi, Vassiliki; Malamateniou, Flora; Prentza, Andriana; Vassilacopoulos, George

    2014-01-01

    Personal Health Records (PHRs), integrated with data from various sources, such as social care data, Electronic Health Record data and genetic information, are envisaged as having a pivotal role in transforming healthcare. These data, lumped under the term 'big data', are usually complex, noisy, heterogeneous, longitudinal and voluminous thus prohibiting their meaningful use by clinicians. Deriving value from these data requires the utilization of innovative data analysis techniques, which, however, may be hindered due to potential security and privacy breaches that may arise from improper release of personal health information. This paper presents a HIPAA-compliant machine learning framework that enables privacy-preserving classification of next-generation PHR data. The predictive models acquired can act as supporting tools to clinical practice by enabling more effective prevention, diagnosis and treatment of new incidents. The proposed framework has a huge potential for complementing medical staff expertise as it outperforms the manual inspection of PHR data while protecting patient privacy.

  5. Habitat use and demography of Mus musculus in a rural landscape of Argentina.

    Science.gov (United States)

    León, Vanina A; Fraschina, Jimena; Guidobono, Juan S; Busch, Maria

    2013-04-01

    The main goal of the paper was to determine the habitat distribution of the house mouse (Mus musculus) within a rural landscape of Buenos Aires province, Argentina. We also studied the seasonal variation in abundance and reproductive activity. The habitats studied were poultry farms, human houses in a small village, cropfields, pastures, cropfield and pasture edges, riparian habitats (streams), railway embankments and woodlots. We captured 817 M. musculus and 690 individuals of 5 native rodent species. M. musculus was captured in poultry farms, houses, riparian habitats, cropfield and borders, but it showed a significantly higher abundance in poultry farms compared to the other habitats. Its presence outside poultry farms was significantly related to the distance to streams and poultry farms. The mean trapping success index of M. musculus did not show significant variations between periods, but the proportion of active males was significantly higher in the spring-summer period than in the autumn-winter period. All captures of M. musculus in cropfields, borders and riparian habitats occurred in the spring-summer period. The capture of M. musculus in many types of habitats suggests that it can disperse outside poultry farms, and streams may be used as corridors. © 2012 Wiley Publishing Asia Pty Ltd, ISZS and IOZ/CAS.

  6. Polymorphism in hybrid male sterility in wild-derived Mus musculus musculus strains on proximal chromosome 17.

    Science.gov (United States)

    Vyskocilová, Martina; Prazanová, Gabriela; Piálek, Jaroslav

    2009-02-01

    The hybrid sterility-1 (Hst1) locus at Chr 17 causes male sterility in crosses between the house mouse subspecies Mus musculus domesticus (Mmd) and M. m. musculus (Mmm). This locus has been defined by its polymorphic variants in two laboratory strains (Mmd genome) when mated to PWD/Ph mice (Mmm genome): C57BL/10 (carrying the sterile allele) and C3H (fertile allele). The occurrence of sterile and/or fertile (wild Mmm x C57BL)F1 males is evidence that polymorphism for this trait also exists in natural populations of Mmm; however, the nature of this polymorphism remains unclear. Therefore, we derived two wild-origin Mmm strains, STUS and STUF, that produce sterile and fertile males, respectively, in crosses with C57BL mice. To determine the genetic basis underlying male fertility, the (STUS x STUF)F1 females were mated to C57BL/10 J males. About one-third of resulting hybrid males (33.8%) had a significantly smaller epididymis and testes than parental animals and lacked spermatozoa due to meiotic arrest. A further one-fifth of males (20.3%) also had anomalous reproductive traits but produced some spermatozoa. The remaining fertile males (45.9%) displayed no deviation from values found in parental individuals. QTL analysis of the progeny revealed strong associations of male fitness components with the proximal end of Chr 17, and a significant effect of the central section of Chr X on testes mass. The data suggest that genetic incompatibilities associated with male sterility have evolved independently at the proximal end of Chr 17 and are polymorphic within both Mmd and Mmm genomes.

  7. Comparison of Trap Types, Placement, and Colors for Monitoring Anthonomus musculus (Coleoptera: Curculionidae) Adults in Highbush Blueberries

    Science.gov (United States)

    Silva, Diego; Salamanca, Jordano; Kyryczenko-Roth, Vera; Alborn, Hans T; Rodriguez-Saona, Cesar

    2018-01-01

    Abstract The cranberry weevil, Anthonomus musculus Say (Coleoptera: Curculionidae), is a key (univoltine) pest of highbush blueberries in the northeast United States. To date, however, no trapping system has been developed to successfully monitor this pest. In 2012–2014, studies were conducted in commercial highbush blueberry farms in New Jersey to 1) evaluate the efficacy of various commercially available traps, designed for other weevil species (e.g., pepper weevil, plum curculio, boll weevil, red palm weevil, and black vine weevil), in capturing A. musculus adults; 2) test whether the relative location of traps within the blueberry canopy affects adult captures and 3) determine the effects of different colored (yellow, white, green, red, blue, brown, and black) sticky traps on weevil captures. For a comparison with existing techniques, we also monitored the number of overwintered adult weevils on blueberry bushes using beat sheet sampling. Of all traps and colors tested, the most A. musculus adults were caught on yellow sticky traps and more adults were captured when these traps were placed at the bottom half of the blueberry canopy, i.e., 0.5–1.0 m above ground. Most weevils were caught on colored traps late in the season (i.e., during bloom), which corresponds mostly to the second (summer) adult generation. Thus, number of overwintered adults caught on traps did not correlate with those on bushes. Although our study identified traps that can be used to capture A. musculus adults, these traps alone (i.e., without semiochemicals) have so far limited applicability for monitoring overwintered adult weevils in highbush blueberries.

  8. Accumulation of silver from drinking water into cerebellum and musculus soleus in mice

    International Nuclear Information System (INIS)

    Pelkonen, Kai H.O.; Heinonen-Tanski, Helvi; Haenninen, Osmo O.P.

    2003-01-01

    In spite of the general toxicity, ecotoxicity and sparsely known metabolism of silver, WHO allows silver ions (Ag) up to 0.1 mg/l in drinking water disinfection. In order to determine the accumulation and distribution of silver in a mammalian body, mice were given for 1 and 2 weeks drinking water containing a 3-fold lower concentration, namely 0.03 mg/l silver ions as silver nitrate labelled with 110m Ag. The silver concentrations in different tissues were analysed by gamma radioactivity. The saturation of tissues with silver seems to occur quickly, as there were no statistical differences between silver contents of mice tissues in spite of the study design that mice were administered silver for 1 or 2 weeks. The highest concentrations were found in musculus soleus (m. soleus), cerebellum, spleen, duodenum, and myocardial muscle in the rank order. Concentrations of silver in musculus gastrocnemius (m. gastrocnemius) were found to correlate negatively with cerebrum and positively with blood and kidneys. The accumulation of silver into organs and tissues important in motor functions may be of relevance especially in emergency and catastrophe situations in which accurate motor functions may be critical. A re-evaluation of the present recommendations on the use of silver salts for disinfection of drinking water might be necessary

  9. Bacillus subtilis RapA phosphatase domain interaction with its substrate, phosphorylated Spo0F, and its inhibitor, the PhrA peptide.

    Science.gov (United States)

    Diaz, Alejandra R; Core, Leighton J; Jiang, Min; Morelli, Michela; Chiang, Christina H; Szurmant, Hendrik; Perego, Marta

    2012-03-01

    Rap proteins in Bacillus subtilis regulate the phosphorylation level or the DNA-binding activity of response regulators such as Spo0F, involved in sporulation initiation, or ComA, regulating competence development. Rap proteins can be inhibited by specific peptides generated by the export-import processing pathway of the Phr proteins. Rap proteins have a modular organization comprising an amino-terminal alpha-helical domain connected to a domain formed by six tetratricopeptide repeats (TPR). In this study, the molecular basis for the specificity of the RapA phosphatase for its substrate, phosphorylated Spo0F (Spo0F∼P), and its inhibitor pentapeptide, PhrA, was analyzed in part by generating chimeric proteins with RapC, which targets the DNA-binding domain of ComA, rather than Spo0F∼P, and is inhibited by the PhrC pentapeptide. In vivo analysis of sporulation efficiency or competence-induced gene expression, as well as in vitro biochemical assays, allowed the identification of the amino-terminal 60 amino acids as sufficient to determine Rap specificity for its substrate and the central TPR3 to TPR5 (TPR3-5) repeats as providing binding specificity toward the Phr peptide inhibitor. The results allowed the prediction and testing of key residues in RapA that are essential for PhrA binding and specificity, thus demonstrating how the widespread structural fold of the TPR is highly versatile, using a common interaction mechanism for a variety of functions in eukaryotic and prokaryotic organisms.

  10. Teratogenic effect of yogurt in mice fetus (Mus musculus

    Directory of Open Access Journals (Sweden)

    Dwisari Dillasamola

    2018-04-01

    Full Text Available Yogurt is one of the dairy products made from lactic acid fermentation by using Lactobacillus bulgaricus and Streptococcus thermophilus. A study on teratogenic effects of yogurt on the white female mice fetus (Mus musculus has been carried out. Pregnant mice used were 20 which divided into 4 groups : the control group, D1, D2, and D3. The treatments giveThe mice were Distidelled water (control, 0.52 yogurt (D1, 1.04  yogurt (D2, and 2.08 g yogurt (D3. Data were analyzed using one-way ANOVA followed by Duncan multiple range test. Results showed that administration of yogurt during pregnancy could affect mother body weight of mice (P 0,05. Observations with Alizarin solution did not show skeletal defects in comparison to the control group. Observations with Bouin’s solution showed defective visceral cleft palate in fetal mice yogurt group D3. This study conclude that yogurt is safe to consume in groups D1 and D2. Yogurt has the potential to cause fetal teratogenic in group D3

  11. The E3 ubiquitin ligase protein associated with Myc (Pam) regulates mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling in vivo through N- and C-terminal domains.

    Science.gov (United States)

    Han, Sangyeul; Kim, Sun; Bahl, Samira; Li, Lin; Burande, Clara F; Smith, Nicole; James, Marianne; Beauchamp, Roberta L; Bhide, Pradeep; DiAntonio, Aaron; Ramesh, Vijaya

    2012-08-31

    Pam and its homologs (the PHR protein family) are large E3 ubiquitin ligases that function to regulate synapse formation and growth in mammals, zebrafish, Drosophila, and Caenorhabditis elegans. Phr1-deficient mouse models (Phr1(Δ8,9) and Phr1(Magellan), with deletions in the N-terminal putative guanine exchange factor region and the C-terminal ubiquitin ligase region, respectively) exhibit axon guidance/outgrowth defects and striking defects of major axon tracts in the CNS. Our earlier studies identified Pam to be associated with tuberous sclerosis complex (TSC) proteins, ubiquitinating TSC2 and regulating mammalian/mechanistic target of rapamycin (mTOR) signaling. Here, we examine the potential involvement of the TSC/mTOR complex 1(mTORC1) signaling pathway in Phr1-deficient mouse models. We observed attenuation of mTORC1 signaling in the brains of both Phr1(Δ8,9) and Phr1(Magellan) mouse models. Our results establish that Pam regulates TSC/mTOR signaling in vitro and in vivo through two distinct domains. To further address whether Pam regulates mTORC1 through two functionally independent domains, we undertook heterozygous mutant crossing between Phr1(Δ8,9) and Phr1(Magellan) mice to generate a compound heterozygous model to determine whether these two domains can complement each other. mTORC1 signaling was not attenuated in the brains of double mutants (Phr1(Δ8,9/Mag)), confirming that Pam displays dual regulation of the mTORC1 pathway through two functional domains. Our results also suggest that although dysregulation of mTORC1 signaling may be responsible for the corpus callosum defects, other neurodevelopmental defects observed with Phr1 deficiency are independent of mTORC1 signaling. The ubiquitin ligase complex containing Pam-Fbxo45 likely targets additional synaptic and axonal proteins, which may explain the overlapping neurodevelopmental defects observed in Phr1 and Fbxo45 deficiency.

  12. GAMBARAN HEMATOLOGI MENCIT (Mus musculus MODEL TOKSISITAS SUBKRONIS

    Directory of Open Access Journals (Sweden)

    Ita Nur Eka Pujiastuti

    2017-06-01

    Full Text Available Garlic commonly is consumed as medicine to prevent or heal illness or to maintain someone's health. Many societies prefer garlic (Allium sativum among other herbal remedies for cholesterol treatment. It consists of several types, and one of them is single bulb garlic used to treat hypertension. There has been, however, no published research reporting the toxicological properties of single bulb garlic. The purpose of this study was to determine subchronic toxic effects of single bulb garlic administered to mice using hematological parameters. The experiment parameters were hemoglobin and hematocrit levels, the number of erythrocytes and leukocytes. Male mice (Mus musculus strain Balb-C were treated with single bulb garlic extract for 28 days with dosage levels of 0% (N , 0.25% (P1 , 0.5% (P2 , 1% (P3 , and 2% (P4 . Single bulb garlic showed no effect on hemoglobin and hematocrit levels but increased the number of erythrocyte and leucocyte. We concluded that single bulb garlic did not cause subchronic toxic effects.

  13. KOLONISASI Mus musculus albino DI LABORATORIUM LOKA LITBANG P2B2 BANJARNEGARA

    Directory of Open Access Journals (Sweden)

    Dewi Marbawati

    2012-11-01

    Full Text Available The improvement and development of science and technologycal in health area must be followed with improvement of requirement of test animal would especially white mouse (Mus musculus albino/mencit. Usage of white mouse (mencit because of relatively easy in handling, the size relatively small, the price relatively cheap, once birth can reach 16- 18, have similiarity in circulation system of human and doesn't have ability to vomit because having spillway in bouncing up, that is why white mouse used for drug trial research. This research expected to obtain stable white mouse colony in laboratory to fulfill requirement of white mouse as subject for research.Research was started in March-November 2008 in Loka Litbang P2B2 Banjarnegara. This research was elementary research with observational design. Data were collected from white mouse growth observation. Result of observation showed reproduction of white mouse after 5 month, from 6 white mouse increased become 17 (increased 2 times more, with number of deaths 11,76%. This white mouse survive until 12 months, but the average was 6 month. Oldest mencit until research ends (November 2008 ranges from 8 months. Daily observation data showed that white mouse can bear children until 6/pregnance. According to literature study mouse can bear children average 68/pregnance. Generaly baby mouse was around 1 gram, heavily horns depend on type (strain of white mouse. Result of white mouse weight showed increase of white mouse's body weight every 2 weeks was 8,6 grams. Observation data of development white mouse showed after 4 days the white mouse hair was seen, specially moustache that was clearly seen, on 5 day whole body was white. At the age of 10 days ear opened, part of other body like external mamilla and genitals become explains looked to be. At the age of 12 days eye started opens and active walking.

  14. Spot 1 Spot 2

    Indian Academy of Sciences (India)

    2011-08-23

    M)[17]. Gel Idx/Pos. 137/F12. Plate [#] Name. [1] T056P10. Rank. Protein Name. Accession No. Protein. Protein. Total Ion. Total Ion. Score. Score. Score. C. I. %. C. I. %. 1. Serum albumin OS=Mus musculus GN=Alb PE=1 SV= ...

  15. Population biology of house mice (Mus musculus L.) on sub ...

    African Journals Online (AJOL)

    1993-05-03

    May 3, 1993 ... Studies on the feral house mouse Mus musculus in habitats ranging from deserts ... Previous studies on mice at Marion Island focused on ..... and food availability) may decrease the rate of development .... Wiley, New York.

  16. Parâmetros morfofisiológicos testiculares de camundongos (Mus musculus suplementados com geleia real Morphophysiological parameters of mice (Mus musculus testicles supplemented with royal jelly

    Directory of Open Access Journals (Sweden)

    A.C.T. Morais

    2009-02-01

    Full Text Available Avaliaram-se os efeitos da geleia real sobre os parâmetros morfofisiológicos testiculares de camundongos (Mus musculus. Utilizaram-se 57 machos Swiss, com quatro meses de idade, distribuídos aleatoriamente em seis tratamentos: T1: solução fisiológica, via intraperitoneal; T2: 0,1mg de geleia real, via intraperitoneal; T3: 0,2mg de geleia real, via intraperitoneal; T4: água destilada, via oral; T5: 0,1mg de geleia real, via oral; e T6: 0,2mg de geleia real, via oral. Após 45 dias de suplementação com geleia real, os animais sacrificados e pesados tiveram seus testículos coletados, incluídos em parafina e corados com hematoxilina/eosina. Não houve diferença entre os tratamentos quanto aos: pesos corporal e testicular, índice gonadossomático, diâmetro tubular, altura do epitélio, comprimento total dos túbulos seminíferos, comprimento tubular por grama de testículo, índices tubulossomático e leydigossomático e valores de proporção volumétrica referentes à túnica própria, epitélio seminífero, vaso sanguíneo e vaso linfático. Foi encontrada diferença entre T1 e T3 em relação aos túbulos seminíferos e ao espaço intertubular.The effects of royal jelly on the morphophysiological parameters of mice (Mus musculus testicles were studied. Fifty-eight male Swiss mice were evaluated. They were four-month old and were randomly distributed in six treatments: T1: physiological solution, intraperitonial route; T2: 0.1mg of royal jelly, intraperitonial route; T3: 0.2mg of royal jelly, intraperitonial route; T4: distilled water, orally; T5: 0.1mg of royal jelly, orally; and T6: 0.2mg of royal jelly, orally. After 45 days of supplementation with royal jelly, the animals were weighted, slaughtered, and the testicles collected, included in paraffin, and stained with haematoxylin-eosin. No differences among treatments were observed for: body and testicular weights, gonadossomatic index, tubular diameter, epithelial height, total

  17. Evolution of major milk proteins in Mus musculus and Mus spretus mouse species: a genoproteomic analysis

    Directory of Open Access Journals (Sweden)

    Panthier Jean-Jacques

    2011-01-01

    Full Text Available Abstract Background Due to their high level of genotypic and phenotypic variability, Mus spretus strains were introduced in laboratories to investigate the genetic determinism of complex phenotypes including quantitative trait loci. Mus spretus diverged from Mus musculus around 2.5 million years ago and exhibits on average a single nucleotide polymorphism (SNP in every 100 base pairs when compared with any of the classical laboratory strains. A genoproteomic approach was used to assess polymorphism of the major milk proteins between SEG/Pas and C57BL/6J, two inbred strains of mice representative of Mus spretus and Mus musculus species, respectively. Results The milk protein concentration was dramatically reduced in the SEG/Pas strain by comparison with the C57BL/6J strain (34 ± 9 g/L vs. 125 ± 12 g/L, respectively. Nine major proteins were identified in both milks using RP-HPLC, bi-dimensional electrophoresis and MALDI-Tof mass spectrometry. Two caseins (β and αs1 and the whey acidic protein (WAP, showed distinct chromatographic and electrophoresis behaviours. These differences were partly explained by the occurrence of amino acid substitutions and splicing variants revealed by cDNA sequencing. A total of 34 SNPs were identified in the coding and 3'untranslated regions of the SEG/Pas Csn1s1 (11, Csn2 (7 and Wap (8 genes. In addition, a 3 nucleotide deletion leading to the loss of a serine residue at position 93 was found in the SEG/Pas Wap gene. Conclusion SNP frequencies found in three milk protein-encoding genes between Mus spretus and Mus musculus is twice the values previously reported at the whole genome level. However, the protein structure and post-translational modifications seem not to be affected by SNPs characterized in our study. Splicing mechanisms (cryptic splice site usage, exon skipping, error-prone junction sequence, already identified in casein genes from other species, likely explain the existence of multiple αs1-casein

  18. On the tear proteome of the house mouse (Mus musculus musculus in relation to chemical signalling

    Directory of Open Access Journals (Sweden)

    Romana Stopkova

    2017-07-01

    Full Text Available Mammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs are not individually unique in Mus musculus musculus. This fact stimulated us to look for other sexually dimorphic proteins that may—in combination with MUPs—contribute to a pool of chemical signals in tears. MUPs and other lipocalins including odorant binding proteins (OBPs have the capacity to selectively transport volatile organic compounds (VOCs in their eight-stranded beta barrel, thus we have generated the tear proteome of the house mouse to detect a wider pool of proteins that may be involved in chemical signalling. We have detected significant male-biased (7.8% and female-biased (7% proteins in tears. Those proteins that showed the most elevated sexual dimorphisms were highly expressed and belong to MUP, OBP, ESP (i.e., exocrine gland-secreted peptides, and SCGB/ABP (i.e., secretoglobin families. Thus, tears may have the potential to elicit sex-specific signals in combination by different proteins. Some tear lipocalins are not sexually dimorphic—with MUP20/darcin and OBP6 being good examples—and because all proteins may flow with tears through nasolacrimal ducts to nasal and oral cavities we suggest that their roles are wider than originally thought. Also, we have also detected several sexually dimorphic bactericidal proteins, thus further supporting an idea that males and females may have adopted alternative strategies in controlling microbiota thus yielding different VOC profiles.

  19. Effect of the [TiO{sub 2}] on the radiation attenuation properties on the human tissue simulator obtained from radiovulcanized natural rubber latex; Efeito da [TiO{sub 2}] nas propriedades de atenuacao da radiacao no simulador de tecido humano obtido do latex de borracha natural radiovulcanizado

    Energy Technology Data Exchange (ETDEWEB)

    Tomimasu, Sumie; Guedes, Selma Matheus Loureiro [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Centro de Tecnologia das Radiacoes]. E-mail: smguedes@net.ipen.br

    2002-07-01

    In this paper was studied the concentration effect of TiO{sub 2} used as filler in the radiation attenuation properties of human tissue simulator obtained from radio vulcanized natural rubber. The studied parameters were the following: the mass stopping power (S{sub mas}), the depth at maximum dose (Z{sub max}), the depth at 50% of maximum dose (R{sub 50}) the radiotherapeutic range (RR{sub 80}) and the electrons range (R). The natural rubber latex was formulated with [n-butyl acrylate ] = e phr and [KOH] = 0,2 phr and radio vulcanized by gamma rays (10 kGy) from {sup 60} Co source. The filler TiO{sub 2} was added (0-16 phr). The natural rubber slabs were obtained by casting method and theirs absorbed dose behavior was studied for each filler concentration using electrons beam (6 MeV) from an accelerator. The results showed that the addition of each phr of filler promoted the decrease of 0.019 cm for Z{sub max}, 0.021 cm for RR{sub 80}, 0.033 cm for R and 0.026 cm for R{sub 50} up to [Ti O{sub 2}] 15 phr. The S{sub mas} increased from 1.72 MeVcm2g-1 to 2.00 MeVcm2g-1 in the filler concentration range of 0-16 phr. The filler must be used in the 12-15 phr range. (author)

  20. Effects of Organomontmorillonite Content on Morphology and Mechanical and Thermal Properties of Poly(2,6-dimethyl-1,4-phenylene oxide/Polyamide-66 Nanocomposites

    Directory of Open Access Journals (Sweden)

    Kunxiao Yang

    2016-01-01

    Full Text Available The nanocomposites consisting of polymer matrix and nanofiller have attracted great attention because of the improved physical properties. In this paper, organomontmorillonite (OMMT was introduced into poly(2,6-dimethyl-1,4-phenylene oxide grafted maleic anhydride (PPO-g-MA compatibilized poly(2,6-dimethyl-1,4-phenylene oxide/polyamide-66 (PPO/PA66 blends by melt extrusion. The morphology of PPO/PA66 nanocomposites with different amounts of OMMT was investigated using transmission electron microscopy (TEM, wide-angle X-ray diffraction (WAXD, and scanning electron microscopy (SEM. The OMMT platelets exhibited an exfoliated structure in the PA66 matrix and an intercalated structure on the surface of PPO domains at low OMMT loading (2phr. However, the exfoliated platelets in matrix were found to transform into intercalated stacks by adding 6 phr of OMMT. The mechanical properties and thermal stability were significantly improved with the coexistence of exfoliated and intercalated OMMT at low OMMT loading (2–4 phr. The exfoliated OMMT platelets imposed a confinement effect on the macromolecular chains and thereby increased the storage modulus and complex viscosity of nanocomposites.

  1. A mutational comparison of adult and adolescent and young adult (AYA) colon cancer.

    Science.gov (United States)

    Tricoli, James V; Boardman, Lisa A; Patidar, Rajesh; Sindiri, Sivasish; Jang, Jin S; Walsh, William D; McGregor, Paul M; Camalier, Corinne E; Mehaffey, Michele G; Furman, Wayne L; Bahrami, Armita; Williams, P Mickey; Lih, Chih-Jian; Conley, Barbara A; Khan, Javed

    2018-03-01

    It is possible that the relative lack of progress in treatment outcomes among adolescent and young adult (AYA) patients with cancer is caused by a difference in disease biology compared with the corresponding diseases in younger and older individuals. There is evidence that colon cancer is more aggressive and has a poorer prognosis in AYA patients than in older adult patients. To further understand the molecular basis for this difference, whole-exome sequencing was conducted on a cohort of 30 adult, 30 AYA, and 2 pediatric colon cancers. A statistically significant difference in mutational frequency was observed between AYA and adult samples in 43 genes, including ROBO1, MYC binding protein 2 (MYCBP2), breast cancer 2 (early onset) (BRCA2), MAP3K3, MCPH1, RASGRP3, PTCH1, RAD9B, CTNND1, ATM, NF1; KIT, PTEN, and FBXW7. Many of these mutations were nonsynonymous, missense, stop-gain, or frameshift mutations that were damaging. Next, RNA sequencing was performed on a subset of the samples to confirm the mutations identified by exome sequencing. This confirmation study verified the presence of a significantly greater frequency of damaging mutations in AYA compared with adult colon cancers for 5 of the 43 genes (MYCBP2, BRCA2, PHLPP1, TOPORS, and ATR). The current results provide the rationale for a more comprehensive study with a larger sample set and experimental validation of the functional impact of the identified variants along with their contribution to the biologic and clinical characteristics of AYA colon cancer. Cancer 2018;124:1070-82. © 2017 American Cancer Society. © 2017 American Cancer Society.

  2. Peroral Echinococcus multilocularis egg inoculation in Myodes glareolus, Mesocricetus auratus and Mus musculus (CD-1 IGS and C57BL/6j)

    DEFF Research Database (Denmark)

    Woolsey, Ian David; Jensen, Per Moestrup; Deplazes, Peter

    2016-01-01

    Echinococcus multilocularis transmission predominantly occurs in Europe between the red fox (Vulpes vulpes) and various species of rodent intermediate hosts. We infected 3 species of rodent, Myodes glareolus (n = 47), Mesocricetus auratus (n = 11) and outbred Mus musculus (CD-1 IGS) (n = 9...

  3. Comparative analysis of Homo sapiens and Mus musculus cyclin-dependent kinase (CDK) inhibitor genes p16 (MTS1) and p15 (MTS2).

    Science.gov (United States)

    Jiang, P; Stone, S; Wagner, R; Wang, S; Dayananth, P; Kozak, C A; Wold, B; Kamb, A

    1995-12-01

    Cyclin-dependent kinase inhibitors are a growing family of molecules that regulate important transitions in the cell cycle. At least one of these molecules, p16, has been implicated in human tumorigenesis while its close homolog, p15, is induced by cell contact and transforming growth factor-beta (TGF-beta). To investigate the evolutionary and functional features of p15 and p16, we have isolated mouse (Mus musculus) homologs of each gene. Comparative analysis of these sequences provides evidence that the genes have similar functions in mouse and human. In addition, the comparison suggests that a gene conversion event is part of the evolution of the human p15 and p16 genes.

  4. Key Amino Acids in the Bacterial (6-4) Photolyase PhrB from Agrobacterium fabrum.

    Science.gov (United States)

    Graf, Dominik; Wesslowski, Janine; Ma, Hongju; Scheerer, Patrick; Krauß, Norbert; Oberpichler, Inga; Zhang, Fan; Lamparter, Tilman

    2015-01-01

    Photolyases can repair pyrimidine dimers on the DNA that are formed during UV irradiation. PhrB from Agrobacterium fabrum represents a new group of prokaryotic (6-4) photolyases which contain an iron-sulfur cluster and a DMRL chromophore. We performed site-directed mutagenesis in order to assess the role of particular amino acid residues in photorepair and photoreduction, during which the FAD chromophore converts from the oxidized to the enzymatically active, reduced form. Our study showed that Trp342 and Trp390 serve as electron transmitters. In the H366A mutant repair activity was lost, which points to a significant role of His366 in the protonation of the lesion, as discussed for the homolog in eukaryotic (6-4) photolyases. Mutants on cysteines that coordinate the Fe-S cluster of PhrB were either insoluble or not expressed. The same result was found for proteins with a truncated C-terminus, in which one of the Fe-S binding cysteines was mutated and for expression in minimal medium with limited Fe concentrations. We therefore assume that the Fe-S cluster is required for protein stability. We further mutated conserved tyrosines that are located between the DNA lesion and the Fe-S cluster. Mutagenesis results showed that Tyr424 was essential for lesion binding and repair, and Tyr430 was required for efficient repair. The results point to an important function of highly conserved tyrosines in prokaryotic (6-4) photolyases.

  5. Key Amino Acids in the Bacterial (6-4 Photolyase PhrB from Agrobacterium fabrum.

    Directory of Open Access Journals (Sweden)

    Dominik Graf

    Full Text Available Photolyases can repair pyrimidine dimers on the DNA that are formed during UV irradiation. PhrB from Agrobacterium fabrum represents a new group of prokaryotic (6-4 photolyases which contain an iron-sulfur cluster and a DMRL chromophore. We performed site-directed mutagenesis in order to assess the role of particular amino acid residues in photorepair and photoreduction, during which the FAD chromophore converts from the oxidized to the enzymatically active, reduced form. Our study showed that Trp342 and Trp390 serve as electron transmitters. In the H366A mutant repair activity was lost, which points to a significant role of His366 in the protonation of the lesion, as discussed for the homolog in eukaryotic (6-4 photolyases. Mutants on cysteines that coordinate the Fe-S cluster of PhrB were either insoluble or not expressed. The same result was found for proteins with a truncated C-terminus, in which one of the Fe-S binding cysteines was mutated and for expression in minimal medium with limited Fe concentrations. We therefore assume that the Fe-S cluster is required for protein stability. We further mutated conserved tyrosines that are located between the DNA lesion and the Fe-S cluster. Mutagenesis results showed that Tyr424 was essential for lesion binding and repair, and Tyr430 was required for efficient repair. The results point to an important function of highly conserved tyrosines in prokaryotic (6-4 photolyases.

  6. A candidate subspecies discrimination system involving a vomeronasal receptor gene with different alleles fixed in M. m. domesticus and M. m. musculus.

    Directory of Open Access Journals (Sweden)

    Robert C Karn

    2010-09-01

    Full Text Available Assortative mating, a potentially efficient prezygotic reproductive barrier, may prevent loss of genetic potential by avoiding the production of unfit hybrids (i.e., because of hybrid infertility or hybrid breakdown that occur at regions of secondary contact between incipient species. In the case of the mouse hybrid zone, where two subspecies of Mus musculus (M. m. domesticus and M. m. musculus meet and exchange genes to a limited extent, assortative mating requires a means of subspecies recognition. We based the work reported here on the hypothesis that, if there is a pheromone sufficiently diverged between M. m. domesticus and M. m. musculus to mediate subspecies recognition, then that process must also require a specific receptor(s, also sufficiently diverged between the subspecies, to receive the signal and elicit an assortative mating response. We studied the mouse V1R genes, which encode a large family of receptors in the vomeronasal organ (VNO, by screening Perlegen SNP data and identified one, Vmn1r67, with 24 fixed SNP differences most of which (15/24 are nonsynonymous nucleotide substitutions between M. m. domesticus and M. m. musculus. We observed substantial linkage disequilibrium (LD between Vmn1r67 and Abpa27, a mouse salivary androgen-binding protein gene that encodes a proteinaceous pheromone (ABP capable of mediating assortative mating, perhaps in conjunction with its bound small lipophilic ligand. The LD we observed is likely a case of association rather than residual physical linkage from a very recent selective sweep, because an intervening gene, Vmn1r71, shows significant intra(subspecific polymorphism but no inter(subspecific divergence in its nucleotide sequence. We discuss alternative explanations of these observations, for example that Abpa27 and Vmn1r67 are coevolving as signal and receptor to reinforce subspecies hybridization barriers or that the unusually divergent Vmn1r67 allele was not a product of fast positive

  7. Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens

    International Nuclear Information System (INIS)

    Singh, Nisha; Halliday, Amy C.; Knight, Matthew; Lack, Nathan A.; Lowe, Edward; Churchill, Grant C.

    2012-01-01

    M. musculus and H. sapiens inositol monophosphatase 1 were cloned, expressed, purified and crystallized. Diffraction data were collected and analysed at resolutions of 2.4 and 1.7 Å, respectively, and the structures were compared in order to identify any structural differences. Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved at resolutions of 2.4 and 1.7 Å, respectively. Comparison of MmIMPase 1 and HsIMPase 1 revealed a core r.m.s. deviation of 0.516 Å

  8. Stress-Induced Cracking of Fluorocarbon Rubber MTL-0002 After Exposure to DS-2

    Science.gov (United States)

    1993-09-01

    recipe is as described in Table I. Table 1. MTL-0002 recipe Component PHR Fluorel 2174 80 Fluorel 2145 20 Carnauba Wax 3 N-990 Carbon Black 30 Maglite D...that this is the V7D proportioning) and that better compression set values could be obtained by using I to 2 phr Carnauba Wax instead of the 3 phr...hydroxide. If additional compression set resistance is desired, the level of Carnauba Wax should also be reduced. However, a post-cure heat treatment is not

  9. The effect of electron beam radiations on testicular damage in mice, Mus musculus

    International Nuclear Information System (INIS)

    Vikram, S.; Nair, Vijay Mala Grover

    2013-01-01

    Adult male Swiss albino mice, Mus musculus (8-10 weeks old) weighing 28±2.5 gm were exposed to varying doses (2-12 Gy) of electron beam radiations and maintained in animal house at 26-28 C. The animals were sacrificed following 35 and 60 days following exposure to electron beam radiations. The LD-50 value, change in the weight and histological details of the testis, sperm count, sperm shape abnormalities and sperm motility were recorded. The data suggests that electron beam radiations is a potential inducer to cause reproductive system dysfunctions which probably may be responsible leading to infertility. (author)

  10. PEMANFAATAN Spirulina platensis SEBAGAI SUPLEMEN PROTEIN SEL TUNGGAL (PST MENCIT (Mus musculus

    Directory of Open Access Journals (Sweden)

    Haryo Kuntoro Adi

    2007-06-01

    Full Text Available The using of Spirulina platensis as Supplement of Single-Celled Protein (SCP to Mice. High protein in Spirulina platensis can be used as a source of Single-Celled Protein. By using mice (Mus musculus as a animal laboratory, the objective of this research is to know the influence of Biomass S. platensis to the increase of body weight of mice. The name of species is Mus musculus, strain is Swiss derivate. Utilized mice were male, 30-50 weighing gram, and 5-7 weeks of age. Treatment group was given by palette and given by biomass of S. Platensis, while control also fed palette but did not give biomass of S. platensis. Yielded biomass was used as food mixed with palette with composition of dry biomass S. platensis with palette was 0%, 10%, 20%, 30%, 40%, and 50%. Data analysis was conducted by using t-tes and analysis of variance. The results showed that by giving of dry biomass of S. platensis affected to the increasement of body weight from the first day until twelfth day of observation, and decrease on the thirteenth and fourteenth day. Pursuant to result of statistic, there is a significant difference (p < 0,05 between before giving and after giving of dry biomass S. platensis during 17 day. By giving dry biomass of S. platensis to mice (Mus musculus at first and second week, it was found the difference of average mice body weight among six concentrations of biomass but did not at the third week. It means that not all concentration of biomass have same effect to the increase of mice body weight as a Single-Celled Protein.

  11. Genetic Variation at Exon 2 of the MHC Class II DQB Locus in Blue Whale (Balaenoptera musculus from the Gulf of California.

    Directory of Open Access Journals (Sweden)

    Diana D Moreno-Santillán

    Full Text Available The genes of the Major Histocompatibility Complex (MHC play an important role in the vertebrate immune response and are among the most polymorphic genes known in vertebrates. In some marine mammals, MHC genes have been shown to be characterized by low levels of polymorphism compared to terrestrial taxa; this reduction in variation is often explained as a result of lower pathogen pressures in marine habitats. To determine if this same reduction in variation applies to the migratory population of blue whales (Balaenoptera musculus that occurs in the Gulf of California, we genotyped a 172 bp fragment of exon 2 of the MHC Class II DQB locus for 80 members of this population. Twenty-two putatively functional DQB allotypes were identified, all of which were homologous with DQB sequences from other cetacean species. Up to 5 putative alleles per individual were identified, suggesting that gene duplication has occurred at this locus. Rates of non-synonymous to synonymous substitutions (ω and maximum likelihood analyses of models of nucleotide variation provided potential evidence of ongoing positive selection at this exon. Phylogenetic analyses of DQB alleles from B. musculus and 16 other species of cetaceans revealed trans-specific conservation of MHC variants, suggesting that selection has acted on this locus over prolonged periods of time. Collectively our findings reveal that immunogenic variation in blue whales is comparable to that in terrestrial mammals, thereby providing no evidence that marine taxa are subject to reduced pathogen-induced selective pressures.

  12. Toxicological Evaluation of Essential Oil From the Leaves of Croton argyrophyllus (Euphorbiaceae) on Aedes aegypti (Diptera: Culicidae) and Mus musculus (Rodentia: Muridae).

    Science.gov (United States)

    Cruz, R C D; Silva, S L C E; Souza, I A; Gualberto, S A; Carvalho, K S; Santos, F R; Carvalho, M G

    2017-07-01

    Plant-derived essential oils can be used as insecticides for vector control. However, to establish their safety, it is necessary to perform toxicological studies. Herein, we evaluated the chemical composition and insecticidal activity of the essential oil from the leaves of Croton argyrophyllus on the third- and fourth-instar larvae and adult Aedes aegypti (L., 1762). We also evaluated the acute toxicity of the essential oil in adult female Mus musculus. The lethal concentration 50 (LC50) and 90 (LC90) of C. argyrophyllus essential oil on larvae of Ae. aegypti were 0.31 and 0.70 mg ml-1, respectively, and 5.92 and 8.94 mg ml-1, respectively, on Ae. aegypti adults. The major components of the essential oil were spathulenol (22.80%), (E)-caryophyllene (15.41%), α-pinene (14.07%), and bicyclogermacrene (10.43%). It also displayed acute toxicity in adults of Mus musculus; the intraperitoneal and oral lethal dose 50 (LD50) were 2,000 mg kg-1 and 2,500 mg kg-1, respectively. The results showed that the essential oil from C. argyrophyllus leaves has insecticidal activity on Ae. aegypti larvae and adults at an average lethal concentration below the median lethal dose needed to cause acute toxicity in the common mouse. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  13. Develop a Prototype Personal Health Record Application (PHR-A) that Captures Information About Daily Living Important for Diabetes and Provides Decision Support with Actionable Advice for Diabetes Self Care

    Science.gov (United States)

    2013-10-01

    PHR-A and will serve as a general marketing and informational website. It will allow the user to create an account and access the system...wheat tag~ and ~mediu-m avocado . MoOcnald"s OeJu:xe Breakfast (Regular S1ze Biscuit) No Syrup &_ l.targarine 39 X Clicking Add Meal/Snack opens up to

  14. Test of Fruit Extract Pare (Momordica charantia L.) to Quality of Ejaculated Spermatozoa Mice (Mus musculus L.)

    Science.gov (United States)

    Fifendy, M.; Indriati, G.

    2018-04-01

    Pare (Momordica charantia L.) can be used in the treatment of various diseases, such as influenza, cancer, anti-inflammatory, anti-HIV, antimitotic and antifertilitas. This study aimed to determine the effect of the herbal bitter (Momordica charantia L.) to ejaculated sperm quality mice (Mus musculus L.). This research was conducted using Completely Randomized Design (CRD) with 4 treatments and 6 replications, water and fed adlibitum. First treatment is given solvent extract. Second treatments extract were given 0.2 gram, third treatment were given 0.4 gram of extracts and fourth treatment were treated exstrac 0.6 gram were orally for 30 days. After the mice decapitated, dissected and take sperm from vas deferens. Then, the sperm preparation determined using the improved Neubauer. Data were analyzed by ANOVA (Analysis of Varians). The results shoured at doses of 0,2 gram, the average sperm count was 19.89. decrease significant when compared with the control in which the average number of sperm 29.13. So with this research the effective doses to decrease sperm count and can be used as a contraception medication dosage was 0,2 gram. It can be conclude that the extract of bitter (Momordica charantia L.) can decrease the quality of the ejaculated sperm of mice (Mus musculus L.)

  15. X-ray structure of a soluble Rieske-type ferredoxin from Mus musculus

    International Nuclear Information System (INIS)

    Levin, Elena J.; Elsen, Nathaniel L.; Seder, Kory D.; McCoy, Jason G.; Fox, Brian G.; Phillips Jr, George N.

    2008-01-01

    The X-ray crystal structure of a soluble Rieske ferredoxin from M. musculus was solved at 2.07 Å resolution, revealing an iron–sulfur cluster-binding domain with similar architecture to the Rieske-type domains of bacterial aromatic dioxygenases. The ferredoxin was also shown to be capable of accepting electrons from both eukaryotic and prokaryotic oxidoreductases. The 2.07 Å resolution X-ray crystal structure of a soluble Rieske-type ferredoxin from Mus musculus encoded by the gene Mm.266515 is reported. Although they are present as covalent domains in eukaryotic membrane oxidase complexes, soluble Rieske-type ferredoxins have not previously been observed in eukaryotes. The overall structure of the mouse Rieske-type ferredoxin is typical of this class of iron–sulfur proteins and consists of a larger partial β-barrel domain and a smaller domain containing Cys57, His59, Cys80 and His83 that binds the [2Fe–2S] cluster. The S atoms of the cluster are hydrogen-bonded by six backbone amide N atoms in a pattern typical of membrane-bound high-potential eukaryotic respiratory Rieske ferredoxins. However, phylogenetic analysis suggested that the mouse Rieske-type ferredoxin was more closely related to bacterial Rieske-type ferredoxins. Correspondingly, the structure revealed an extended loop most similar to that seen in Rieske-type ferredoxin subunits of bacterial aromatic dioxygenases, including the positioning of an aromatic side chain (Tyr85) between this loop and the [2Fe–2S] cluster. The mouse Rieske-type ferredoxin was shown to be capable of accepting electrons from both eukaryotic and prokaryotic oxidoreductases, although it was unable to serve as an electron donor for a bacterial monooxygenase complex. The human homolog of mouse Rieske-type ferredoxin was also cloned and purified. It behaved identically to mouse Rieske-type ferredoxin in all biochemical characterizations but did not crystallize. Based on its high sequence identity, the structure of the

  16. Effect of Physalis peruviana "tomatillo" fruit extract in Mus musculus var. swis with induced hyperlipidemi.

    Directory of Open Access Journals (Sweden)

    Julio Campos Florián

    2011-06-01

    Full Text Available The objective of this research was to determine the hypolipidemic activity of the fruit of Physalis peruviana "tomatillo" in a model of acute hyperlipidemia induced by triton. Mus musculus var. swis males as experimental animals. We worked with four groups of mice, the white group received distilled water orally and saline intraperitoneally, the control group received distilled water orally and intraperitoneally triton, the problem group 1 received orally 0.05g/100g Physalis peruviana extract intraperitoneally and triton and the problem group 2 received orally 0.2g/100g extract of Physalis peruviana and triton intraperitoneally. After 24 hours of administering the treatments were performed measurements of serum cholesterol and triglycerides. Mean levels of cholesterol (mg/dL were: 58.87 ± 11.54 (white, 121.71 ± 15.00 (control, 58.08 ± 9.21 (Problem 1 and 66 78 ± 16.77 (Problem 2. Average levels of triglycerides (g /L were: 0.48 ± 0.07 (white, 1.84 ± 0.18 (control, 0.34 ± 0.10 (Problem 1 and 0.94 ± 0.25 (Problem 2. We found significant reductions (p <0.000 concentrations of both cholesterol and triglycerides in relation to those obtained in the group treated only with Triton.

  17. Pemanfaatan zeolit sebagai bahan pengisi dalam pembuatan karet sponge untuk tatakan sepatu

    Directory of Open Access Journals (Sweden)

    Herminiwati Herminiwati

    2007-06-01

    Full Text Available The purpose of this research was to study the effect of zeolite as filler in the preparation of sponge rubber for shoe insocks. During wearing period of shoes, unpleasant odor often smell for the result of sweat accumulation and their decomposition by microorganism. Besides as rubber filler, zeolit has odor adsorption function. The formula of rbber sponge could be as the following : crepe rubber 100 phr, paraffinic oil 20 phr, ZnO 10 phr, stearic acid 5 phr, MBTS 1 phr, TMTD 0.25 phr, anti-oxidant 1 phr and sulphur 2 phr. To obtain the best sponge rubber formula, some experiment was done by formulating variation of zeolite and blowing agent AZDM in the amount of 25, 50, 75 phr and 5, 10, 15 phr respectively. The vulcanization process was carried-out at temperature 140oC for minutes by compression moulding. The research showed that the best formula with high odor absorption was consist of zeolite 50 phr and blowing agent AZDM 5 phr. The sponge rubber had odor adsorption 93,47%, and the physical properties were as follow: tensile strength 2.018 N/mm2, elongation at break 251%, tear strength 1.029 N/mm2, density 0.717 g/cm3, and compression set 36.38%. Odor absorption was tested by using gas chromatography.

  18. Food preferences of wild house-mice (Mus musculus L.)*

    Science.gov (United States)

    Rowe, F. P.; Bradfield, A.; Redfern, R.

    1974-01-01

    The relative acceptance of various plain foods by wild house-mice (Mus musculus L.) was compared in laboratory choice tests. The palatability of glycerine and six oils, each included at 5% in pinhead oatmeal, was compared in a similar manner. The most favoured food was found to be whole canary seed (Phalaris canariensis). Pinhead oatmeal and wheat were also comparatively well accepted. Glycerine, corn oil, arachis oil and mineral oil were more palatable than either olive, linseed or cod-liver oils. The results of the choice tests are considered in relation to the use of poison baits for the control of free-living mice. PMID:4531454

  19. Penggunaan precipitated calcium carbonate (PCC sebagai filler untuk sol karet sepatu olah raga

    Directory of Open Access Journals (Sweden)

    Herminiwati

    2010-12-01

    Full Text Available Abstract The objective of the research was to investigate the utilization of Precipitated Calcium Carbonate (PCC as filler in producing sport shoe rubber soles. PCC is a white filler needed for production of nonblack colour rubber products. There are four types of PCC that have been used including two local PCC from Wonosari and East Java, and two imported PCC from Japan and Taiwan. The amount of PCC added into the sport shoe sole rubber compound was varied in 30,45,60,75 and 90 per hundred rubber (phr. The compounding was carried-out by using two roll mills machine, and the compound was subsequently measured their optimum vulcanization time by using rheometer. The produced compound was then subjected to vulcanistion process by using hydrolic press at temperature 1500C and pressure 150 kg/ cm2. The quality of shoes sole vulcanisates were compare to standard quality of SNI. 12-7075-2005 about cemented system sport shoes. The results indicated that the best formula of rubber compound for sport shoes sole were made by using NR 80 phr, NBR 20 phr, paraffinic oil 10 phr, aluminium silicate 30 phr, ZnO 5 phr, TiO2 10 phr, stearic acid 1 phr, vulkanox SP 1 phr, paraffin wax 1 phr, TMTD 0,5 phr, CBS 2 phr, sulphur 1,2 phr with the amount of PCC Actifort 700 of 45 phr. The best formula meet the requirement SNI 12-7075-2005 and they were characterized by tensile sterength 16,79 N/mm2, elongation at break 529,92% tear resistance 9,06 N/mm2, specific gravity 1,28 g/cm3, hardness 55 shore A, Grasselli absrassion resistancing filler. The local PCC from Wonosari can be used for substitution of the imported PCC as the white filler for the production of rubber compound sport shoes sole. However, particle size reduction and coating or surface treatment of local PCC were needed for improving the quality and the role of reinforcing filler.

  20. The screening of parasites and viral pathogens of small mammals from a farm in southern Finland, and genetic identification of the Finnish house mouse, Mus musculus

    Czech Academy of Sciences Publication Activity Database

    Laakkonen, J.; Kallio-Kokko, M.; Vapalahti, O.; Vaheri, A.; Vyskočilová, M.; Munclinger, P.; Macholán, Miloš; Henttonen, H.

    2007-01-01

    Roč. 44, - (2007), s. 202-208 ISSN 0003-455X EU Projects: European Commission(XE) 10284 - EDEN Institutional research plan: CEZ:AV0Z50450515 Keywords : Mus musculus Subject RIV: EG - Zoology Impact factor: 1.537, year: 2007

  1. A multicopy phr-plasmid increases the ultraviolet resistance of a recA strain of Escherichia coli

    International Nuclear Information System (INIS)

    Yamamoto, K.; Satake, M.; Shinagawa, H.

    1984-01-01

    It has been previously reported that the ultraviolet sensitivity of recA strains of Escherichia coli in the dark is suppressed by a plasmid pKY1 which carries the phr gene, suggesting that this is due to a novel effect of photoreactivating enzyme (PRE) of E. coli in the dark. In this work, it is observed that an increase of UV-resistance by pKY1 in the dark is not apparent in strains with a mutation in either uvrA, uvrB, uvrC, lexA, recBC or recF. The sensitivity of recA lexA and recA recBC multiple mutants to UV is suppressed by the plasmid but that of recA uvrA, recA uvrB and recA uvrC is not. Host-cell reactivation of UV-irradiated lambda phage is slightly more efficient in the recA/pKY1 strain compared with the parental recA strain. On the other hand, the recA and recA/pKY1 strains do not differ significantly in the following properties: Hfr recombination, induction of lambda by UV, and mutagenesis. It is suggested that dark repair of PRE is correlated with its capacity of excision repair. (Auth.)

  2. Efficacy of drugs against Giardia muris in mice Mus musculus naturally infected/
    Eficácia de drogas contra Giardia muris em camundongos Mus musculus naturalmente infectados

    OpenAIRE

    Silvia Gonzalez Monteiro; Régis Adriel Zanette; Camila Belmonte Oliveira; Marcos Kipper da Silva; Aleksandro Schafer da Silva

    2008-01-01

    This study aimed to evaluate the efficacy of metronidazole, fenbendazole and secnidazole against Giardia muris in mice naturally infected. Forty mice of the species Mus musculus were divided in four groups of ten each, being group A non treated, the control group and groups B, C and D treated with 4mg/ml of metronidazole, fenbendazole and secnidazole, respectively. Two feces collection, on day 0 and on day 10 after treatment, were done in order to evaluate the efficacy of the drugs. Samples w...

  3. Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.

    OpenAIRE

    Sanford, J; Forrester, L; Chapman, V; Chandley, A; Hastie, N

    1984-01-01

    The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic...

  4. Protein: MPA1 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available MPA1 TLR signaling molecules Rsad2 Vig1 Radical S-adenosyl methionine domain-containing pr...otein 2 Viperin, Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible 10090 Mus musculus 58185 Q8CBB9 21435586 ...

  5. Rap phosphatase of virulence plasmid pXO1 inhibits Bacillus anthracis sporulation.

    Science.gov (United States)

    Bongiorni, Cristina; Stoessel, Ricarda; Shoemaker, Dorinda; Perego, Marta

    2006-01-01

    This study shows that the Bacillus anthracis pXO1 virulence plasmid carries a Rap-Phr system, BXA0205, which regulates sporulation initiation in this organism. The BXA0205Rap protein was shown to dephosphorylate the Spo0F response regulator intermediate of the phosphorelay signal transduction system that regulates the initiation of the developmental pathway in response to environmental, metabolic, and cell cycle signals. The activity of the Rap protein was shown to be inhibited by the carboxy-terminal pentapeptide generated through an export-import processing pathway from the associated BXA0205Phr protein. Deregulation of the Rap activity by either overexpression or lack of the Phr pentapeptide resulted in severe inhibition of sporulation. Five additional Rap-Phr encoding systems were identified on the chromosome of B. anthracis, one of which, BA3790-3791, also affected sporulation initiation. The results suggest that the plasmid-borne Rap-Phr system may provide a selective advantage to the virulence of B. anthracis.

  6. Rap Phosphatase of Virulence Plasmid pXO1 Inhibits Bacillus anthracis Sporulation†

    Science.gov (United States)

    Bongiorni, Cristina; Stoessel, Ricarda; Shoemaker, Dorinda; Perego, Marta

    2006-01-01

    This study shows that the Bacillus anthracis pXO1 virulence plasmid carries a Rap-Phr system, BXA0205, which regulates sporulation initiation in this organism. The BXA0205Rap protein was shown to dephosphorylate the Spo0F response regulator intermediate of the phosphorelay signal transduction system that regulates the initiation of the developmental pathway in response to environmental, metabolic, and cell cycle signals. The activity of the Rap protein was shown to be inhibited by the carboxy-terminal pentapeptide generated through an export-import processing pathway from the associated BXA0205Phr protein. Deregulation of the Rap activity by either overexpression or lack of the Phr pentapeptide resulted in severe inhibition of sporulation. Five additional Rap-Phr encoding systems were identified on the chromosome of B. anthracis, one of which, BA3790-3791, also affected sporulation initiation. The results suggest that the plasmid-borne Rap-Phr system may provide a selective advantage to the virulence of B. anthracis. PMID:16385039

  7. Chromosomal heterozygosity and fertility in house mice (Mus musculus domesticus) from Northern Italy.

    OpenAIRE

    Hauffe, H C; Searle, J B

    1998-01-01

    Following the discovery of over 40 Robertsonian (Rb) races of Mus musculus domesticus in Europe and North Africa, the house mouse has been studied extensively as an ideal model to determine the chromosomal changes that may cause or accompany speciation. Current models of chromosomal speciation are based on the assumption that heterozygous individuals have a particularly low fertility, although recent studies indicate otherwise. Despite their importance, fertility estimates for the house mouse...

  8. Interval training by normobaric hypoxia accelerates the reinnervation of musculus extensor digitorum longus in mice

    DEFF Research Database (Denmark)

    Vardya, Irina; (Vard'ya); Mospanova, Svetlana V.

    2000-01-01

    Dokl Biol Sci. 2000 Mar-Apr;371:112-4. Interval training by normobaric hypoxia accelerates the reinnervation of musculus extensor digitorum longus in mice. Vard'ya IV , Mospanova SV , Portnov VV , Balezina OP , Koshelev VB . Department of Human and Animal Physiology, Faculty of Biology, Moscow St...... State University, Russia. PMID: 10833635 [PubMed - indexed for MEDLINE] Udgivelsesdato: 2000...

  9. Pengaruh Lendir Bekicot (Achatina fulica terhadap Jumlah Sel Fibroblas pada Penyembuhan Luka Sayat Studi Eksperimental pada Kulit Mencit (Mus musculus

    Directory of Open Access Journals (Sweden)

    Perez Wahyu Purnasari

    2012-12-01

    Design and methods: The study is an experimental research with post test only randomized control group design. The subjects were 18 Mus musculus strain mice that were divided into 3 groups. 1st group as a control group of untreated, 2nd group was given povidone iodine 10%, and 3rd group was given land snails’ mucus during 5th days. On the 6th day the mices were killed for histological study. Results: The data showed that average number of fibroblast in 1st group is 93,85; 2nd group is 126,37; and 3rd is group 217,5. The results of one way anova statistical test showed significant difference among the three groups p = 0,000. The results of post hoc test showed significant difference in all groups (p < 0,05. Conclusion: The mucus of land snail is influence the number of fibroblast in healing cuts (Sains Medika, 4(2:195-203.

  10. Comparison of trap types, placement, and colors for monitoring Anthonomus musculus (Coleoptera: Curculionidae) adults in highbush blueberries

    Science.gov (United States)

    Cranberry weevil, Anthonomus musculus Say (Coleoptera: Curculionidae), is a key pest of highbush blueberries in the northeast USA. To date, however, no trapping system has been developed to successfully monitor this pest. In 2012-2014, studies were conducted in commercial blueberry farms in New Jers...

  11. Pembuatan sol karet lembaran untuk sandal

    Directory of Open Access Journals (Sweden)

    Herminiwati Herminiwati

    1999-12-01

    Full Text Available The purpose of the research was to find best formulation for rubber sole sheeting for sandal. The formulation of the compounds were made by variation ratio of natural rubber and sintetic rubber SBR as follow : 100/0, 75/25, 50/50, 25/75 and 0/100, where as the variation ratio of carbon black N330 and aluminium silicate filler 100/0, 60/40 and 20/80 respectively. Compounding process was carried out on two roll mill. The rubber compounds were vulcanized at temperature 150oC and pressure of 150 kg/cm2 with curing time as indicated by curometer. Vulcanized rubber were tested based on SNI. 06-0899-1989 : Rubber sheeting for sole. The best formulation was consist of natural rubber 75 phr, sintetic rubber SBR 25 phr, stearic acid 3 phr, zinc oxide 5 phr, Minarex B oil 5 phr, car bon black 100 phr, MBTS 1 phr, PBN 1 phr and sulfur 2 phr. The physical properties of the best compound were : tensile strength 185,42 kg/cm2, elongation at break 276,64%, tear resistance 158,90 kg/cm2, hardness 74 shore A, density 1,21 g/cm3, Grasselli abrasion resistance 0,326 mm3/kgm, permanent set 7,78% and no crack on the flex cracking test. The best compound could meet the requirements of SNI. 06-0899-1989.

  12. Genomic resources for wild populations of the house mouse, Mus musculus and its close relative Mus spretus

    Science.gov (United States)

    Harr, Bettina; Karakoc, Emre; Neme, Rafik; Teschke, Meike; Pfeifle, Christine; Pezer, Željka; Babiker, Hiba; Linnenbrink, Miriam; Montero, Inka; Scavetta, Rick; Abai, Mohammad Reza; Molins, Marta Puente; Schlegel, Mathias; Ulrich, Rainer G.; Altmüller, Janine; Franitza, Marek; Büntge, Anna; Künzel, Sven; Tautz, Diethard

    2016-01-01

    Wild populations of the house mouse (Mus musculus) represent the raw genetic material for the classical inbred strains in biomedical research and are a major model system for evolutionary biology. We provide whole genome sequencing data of individuals representing natural populations of M. m. domesticus (24 individuals from 3 populations), M. m. helgolandicus (3 individuals), M. m. musculus (22 individuals from 3 populations) and M. spretus (8 individuals from one population). We use a single pipeline to map and call variants for these individuals and also include 10 additional individuals of M. m. castaneus for which genomic data are publically available. In addition, RNAseq data were obtained from 10 tissues of up to eight adult individuals from each of the three M. m. domesticus populations for which genomic data were collected. Data and analyses are presented via tracks viewable in the UCSC or IGV genome browsers. We also provide information on available outbred stocks and instructions on how to keep them in the laboratory. PMID:27622383

  13. STRUKTUR HISTOLOGI HATI MENCIT(Mus musculus L. SETELAH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhapidhophora pinnata Schott

    Directory of Open Access Journals (Sweden)

    Ni Komang Tia Pramesti

    2017-09-01

    Full Text Available ABSTRACT Rhapidhophora pinnata, Schott is a traditional medical plant that has been use remedy for treatment of hypertension, stroke. Aims of  this research is to determine the effect of Rhapidhophora pinnata Schott leaf extract toward  mouse (Mus musculus L liverhistological structure. This research used completely randomized  design with 28 female mice that were divide into 4 groups of  P0 (control received 0,9% NaCl, group P1, P2, and P3 received 50, 100 and 150 mg/kg bw respectively by oral administration. The treatment were given daily for 14 days. All of the mice were sacrificed by ether after 15 days. The liver were examined for their histopathological changes, namely fatty degeneration, hydropic degenaration and necrosis. Results were statistically analised by Kruskal-Wallis method. Treatment groups showed no significant differences with regard of liver histopatological changes, however hemorrhage, sinusoid congestion and inflamatory cell infiltration were found in liver.   Keyword : Rhapidhophora pinnata Schott, liver, mice

  14. NCBI nr-aa BLAST: CBRC-CJAC-01-1133 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1133 ref|NP_068677.2| hairless protein [Mus musculus] sp|Q61645|HAIR_MOUSE Protein hair...less gb|AAH49182.1| Hairless [Mus musculus] gb|EDL35888.1| hairless, isoform CRA_a [Mus musculus] gb|EDL35889.1| hai...rless, isoform CRA_a [Mus musculus] gb|EDL35890.1| hairless, isoform CRA_a [Mus musculus] NP_068677.2 5.1 44% ...

  15. A study of using polythiol compounds and 2-ethyl-hexyl-acrylate with carbon tetrachloride as sensitizers for radiation vulcanization of natural rubber latex

    International Nuclear Information System (INIS)

    Polsuksiri, C.

    1989-01-01

    Experiments on using 3 different compounds of polythiol and an acrylate as sensitizer for radiation vulcanization were conducted. It was found that 1,4 butane diol propane tris-3-mercapto propionate showed the tendency to be a good sensitizer. The tensile strength of the rubber film prepared from the irradiated latex was found to be 14 MPa at sensitizer concentration of 1 phr and radiation dose of 45 kGy. As for 2-ethyl hexyl acrylate (2EHA), the maximum tensile strength of rubber film was found to be 23 MPa at concentration of 3 phr and radiation dose of 35 kGy. The mixture of 2 EHA and CCl 4 at various ratio was also used as sensitizer. The optimum ratio was found to be 5:1 at concentration of 6 phr and radiation dose of 15 kGy. The maximum tensile strength was as high as 25 MPa. The study also revealed that the radiation vulcanized latex with crosslink density of about 18x10 18 C.L./cm 3 would give the rubber film of highest tensile strength

  16. Lem kompon karet untuk sepatu kulit yang dibuat dengan proses vulkanisasi

    Directory of Open Access Journals (Sweden)

    Herminiwati Herminiwati

    2008-12-01

    Full Text Available The purpose of this research was to find rubber compound adhesive formula for preparing leather shoes of direct vulcanizing process. Leather shoes manufactured by vulcanizing process need a suitable rubber compound adhesive for joining the leather uppers and unvulcanized rubber compound soles. The amount of tackifier in rubber compound adhesive influence the adhesive bonding strength. In preparing the adhesive formula, the effect of coumarone resin addition as tackifier were varies in the mount of 5 , 10 and 15 phr respectively. Rubber compound adhesive was produced by two roll mill machine, after that compound were dissolved in wash benzene with ratio 1 : 10. The prepared adhesive then was applied to manufacture leather shoes using vulcanization moulding at temperature 1700 C and 150 kg/cm2 pressure for 8 minutes. The best adhesive formula could be as the following : natural rubber 100 phr, calcium silicate 5 phr, coumarone resin 10 phr, zinc oxide 10 phr, stearic acid 2 phr, AOSP 2 phr, MBTS 0,8 phr; TMTD 0,2 phr and sulphur 2 phr. The best formula had the peeling strength of the outsole 65,25 kg, peeling strength of the insole 1553 g/cm, and could meet the requirements of JIS 5050, 1984 for Leather shoes. Viscosity of the formula was 2250 cp.

  17. The Effect of Curry Leaves (Murayya Koenigii L. on Blood Glucose Levels In Alloxan Diabetic Mice (Mus Musculus

    Directory of Open Access Journals (Sweden)

    Fauziah Fauziah

    2014-07-01

    Full Text Available This study was conducted to determine the effect of ethanol extract of curry leaves (Murraya koenigii L. on blood glucose levels in alloxan diabetic mice (Mus musculus. The diabetic conditions were made by giving alloxan 75 mg/kg body weight (BW and the hypoglycemic effects of extract of curry leaves given with various doses.   This study used 24 male mice strain Balb/c in four groups of treatment with six replications, namely the negative control group, the ethanol extract of curry leaf tree 50% mL/10g body weight group, 70% mL/10g body weight group and 90% mL/10g body weight group. The treatment was given orally by using a gastric sonde for 14 days. Blood samples were taken through the sinus caudalis using a scissors. Blood glucose level was measured at 1st , the 8th and the 24th of treatment using blood glucose test strips and Nesco® Multicheck apparatus. Blood glucose data were analyzed by one way ANOVA (Analysis of Variants and followed by Tuckey test at significance level of 5%. The result showed that treatment of ethanol extract of curry leaves (Murraya koenigii at various doses significantly affected the decrease on blood glucose levels of mice (Mus musculus alloxan diabetic.

  18. Efficacy of drugs against Giardia muris in mice Mus musculus naturally infected/ Eficácia de drogas contra Giardia muris em camundongos Mus musculus naturalmente infectados

    Directory of Open Access Journals (Sweden)

    Silvia Gonzalez Monteiro

    2008-08-01

    Full Text Available This study aimed to evaluate the efficacy of metronidazole, fenbendazole and secnidazole against Giardia muris in mice naturally infected. Forty mice of the species Mus musculus were divided in four groups of ten each, being group A non treated, the control group and groups B, C and D treated with 4mg/ml of metronidazole, fenbendazole and secnidazole, respectively. Two feces collection, on day 0 and on day 10 after treatment, were done in order to evaluate the efficacy of the drugs. Samples were analyzed by the centrifugal-flotation method with zinc sulfate. Efficacy of 97,05% for metronidazole, 98,30% for fenbendazole and 100% for secnidazole were observed in the study. According to the results it was concluded that the tested drugs were effective for the treatment of mice parasitized by Giardia muris.Este estudo visou avaliar a eficácia do metronidazol, fenbendazole e secnidazol contra Giardia muris em camundongos naturalmente infectados. Foram utilizados 40 camundongos da espécie Mus musculus divididos em quatro grupos de 10 animais cada, sendo grupo A, grupo controle, não tratados, e grupos B, C e D tratados com 4mg/ml de metronidazol, fenbendazole e secnidazol, respectivamente. Para avaliar a eficácia dos medicamentos foram realizadas duas coletas de fezes uma no dia zero e outra 10 dias após tratamento. As amostras foram processadas e analisadas a partir do método de centrífugo-flutuação com sulfato de zinco. No estudo observou-se eficácia de 97,05% para metronidazol, 98,30% para fenbendazole e 100% para secnidazol no tratamento de giardiase murina. Com base nos resultados concluí-se que as drogas testadas apresentaram eficácia no tratamento de camundongos parasitados por Giardia muris.

  19. PENGARUH PEMBERIAN “KOMBUCHA” TEH ROSELLA TERHADAP PROFIL DARAH MENCIT (Mus musculus L)

    OpenAIRE

    Mukhani Dwi Hidayanti; Sussi Astuti; Maria Erna Kustyawati

    2015-01-01

    “Kombucha” rosella tea is a functional fermented beverage product solution from the petals of rosella tea and sugar using a microbial starter “Kombucha” (Acetobacter xylinum and several kind of yeast). The objective of the experiment was to determine the effect of “Kombucha” rosella tea on the blood profile of mice (Mus musculus L). The experiment compiled in a completely randomized design with 4 treatments dose “Kombucha” rosella tea was 0,73 ml/20 g BB mice (distilled water) (K), 0,36 ml...

  20. Role of secretory phospholipase A(2) in rhythmic contraction of pulmonary arteries of rats with monocrotaline-induced pulmonary arterial hypertension.

    Science.gov (United States)

    Tanabe, Yoshiyuki; Saito-Tanji, Maki; Morikawa, Yuki; Kamataki, Akihisa; Sawai, Takashi; Nakayama, Koichi

    2012-01-01

    Excessive stretching of the vascular wall in accordance with pulmonary arterial hypertension (PAH) induces a variety of pathogenic cellular events in the pulmonary arteries. We previously reported that indoxam, a selective inhibitor for secretory phospholipase A(2) (sPLA(2)), blocked the stretch-induced contraction of rabbit pulmonary arteries by inhibition of untransformed prostaglandin H(2) (PGH(2)) production. The present study was undertaken to investigate involvement of sPLA(2) and untransformed PGH(2) in the enhanced contractility of pulmonary arteries of experimental PAH in rats. Among all the known isoforms of sPLA(2), sPLA(2)-X transcript was most significantly augmented in the pulmonary arteries of rats with monocrotaline-induced pulmonary hypertension (MCT-PHR). The pulmonary arteries of MCT-PHR frequently showed two types of spontaneous contraction in response to stretch; 27% showed rhythmic contraction, which was sensitive to indoxam and SC-560 (selective COX-1 inhibitor), but less sensitive to NS-398 (selective COX-2 inhibitor); and 47% showed sustained incremental tension (tonic contraction), which was insensitive to indoxam and SC-560, but sensitive to NS-398 and was attenuated to 45% of the control. Only the rhythmically contracting pulmonary arteries of MCT-PHR produced a substantial amount of untransformed PGH(2), which was abolished by indoxam. These results suggest that sPLA(2)-mediated PGH(2) synthesis plays an important role in the rhythmic contraction of pulmonary arteries of MCT-PHR.

  1. NCBI nr-aa BLAST: CBRC-MMUS-06-0135 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0135 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9EP51|VN1B1_MOUSE Vomeronas...al type-1 receptor B1 (Vomeronasal type-1 receptor A5) (Vomeronasal receptor 2) (Ph...eromone receptor VN2) gb|AAG42083.1|AF291489_1 vomeronasal receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 ...[Mus musculus] gb|AAI07184.1| Vomeronasal 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 1e-139 80% ...

  2. NCBI nr-aa BLAST: CBRC-MMUS-06-0136 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0136 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9EP51|VN1B1_MOUSE Vomeronas...al type-1 receptor B1 (Vomeronasal type-1 receptor A5) (Vomeronasal receptor 2) (Ph...eromone receptor VN2) gb|AAG42083.1|AF291489_1 vomeronasal receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 ...[Mus musculus] gb|AAI07184.1| Vomeronasal 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 1e-175 100% ...

  3. NCBI nr-aa BLAST: CBRC-MMUS-06-0141 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0141 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9EP51|VN1B1_MOUSE Vomeronas...al type-1 receptor B1 (Vomeronasal type-1 receptor A5) (Vomeronasal receptor 2) (Ph...eromone receptor VN2) gb|AAG42083.1|AF291489_1 vomeronasal receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 ...[Mus musculus] gb|AAI07184.1| Vomeronasal 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 1e-135 78% ...

  4. The digital divide in adoption and use of a personal health record.

    Science.gov (United States)

    Yamin, Cyrus K; Emani, Srinivas; Williams, Deborah H; Lipsitz, Stuart R; Karson, Andrew S; Wald, Jonathan S; Bates, David W

    2011-03-28

    Personal health records (PHRs) offer the potential to improve the patient experience and the quality of patient care. However, the "digital divide," the population-level gap in Internet and computer access, may prevent certain groups from accessing the PHR. We conducted a cross-sectional analysis of a PHR within a northeastern health system. We compared adopters (ie, those activating a PHR account online) with nonadopters (ie, those who see a physician offering the PHR but do not activate an account). We further categorized adopters by intensity of PHR use, measured by number of log-ins and number of messages sent to physicians' practices. As of September 30, 2009, among 75,056 patients, 43% had adopted the PHR since 2002. Blacks and Hispanics were less likely to adopt the PHR compared with whites (odds ratio [OR], 0.50; 95% confidence interval [CI], 0.45-0.55; and 0.64; 0.57-0.73, respectively), and those with lower annual income were less likely to adopt the PHR than were those with higher income. Compared with nonadopters, adopters were more likely to have more than 2 comorbidities (OR, 1.27; 95% CI, 1.17-1.30). Use of an aggressive marketing strategy for PHR enrollment increased adoption nearly 3-fold (OR, 2.92; 95% CI, 1.58-5.40). Intensity of use was best predicted by increasing number of comorbidities, followed by race/ethnicity (whites more than blacks and Hispanics) and insurance status. We found no association between income and log-in frequency or secure messages sent. Despite increasing Internet availability, racial/ethnic minority patients adopted a PHR less frequently than white patients, and patients with the lowest annual income adopted a PHR less often than those with higher incomes. Among adopters, however, income did not have an effect on PHR use.

  5. NCBI nr-aa BLAST: CBRC-OCUN-01-0645 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0645 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9EP51|VN1B1_MOUSE Vomeronas...al type-1 receptor B1 (Vomeronasal type-1 receptor A5) (Vomeronasal receptor 2) (Ph...eromone receptor VN2) gb|AAG42083.1|AF291489_1 vomeronasal receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 ...[Mus musculus] gb|AAI07184.1| Vomeronasal 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 3e-46 53% ...

  6. BIOAKTIFITAS BUNGA SEPATU (Hibiscus rosa-sinensis L. TERHADAP KADAR KOLESTEROL DARAH MENCIT (Mus musculus YANG MENGALAMI HIPERKOLESTEROLEMIA DENGAN DIET TINGGI LEMAK

    Directory of Open Access Journals (Sweden)

    Rinza Rahmawati Samsudin

    2017-12-01

      Tujuan penelitian ini adalah untuk mengetahui pengaruh bioaktifitas ekstrak bunga sepatu (Hibiscus rosa-sinensis l. terhadap  kadar kolesterol darah mencit (Mus musculus yang mengalami hiperkolesterolemia dengan diet tinggi lemak. Penelitian ini merupakan penelitian eksperimental murni, dengan menggunakan rancangan randomized post-test only control group design. Bunga sepatu mengandung pigmen antosianin yang termasuk golongan flavonoid yang berperan sebagai antioksidan. Sebanyak dua puluh delapan mencit dibagi menjadi empat kelompok (K1, K2, K3 dan K4. Kelompok K1 adalah kelompok yang mendapat perlakukan pakan tinggi lemak dan aquadest setelah aklimatasi. Kelompok K2, K3, dan K4 adalah kelompok  terapi yang mendapat perlakukan pakan tinggi lemak, ekstrak bunga septum sebanyak 1 ml dan aquadest setelah aklimatasi. Pemberian pakan tinggi lemak berlangsung selama 28 hari. Selama penelitian berlangsung penimbangan berat badan tikus dilakukan sebanyak lima kali. Hasil penelitian menunjukan bahwa pemberian 1 ml ekstrak bunga sepatu selama dua puluh delapan hari dapat menurunkan kadar kolesterol mencit dimana nilai p<0.05.   Kata kunci: ekstrak bunga sepatu, kadar kolesterol, diet tinggi lemak

  7. EFEK ANTIFERTILITAS EKSTRAK AKAR SOM JAWA (Talinum paniculatum Gaertn. PADA MENCIT (Mus musculus L. JANTAN

    Directory of Open Access Journals (Sweden)

    Tetri Widiyani

    2012-10-01

    Full Text Available Talinum paniculatum Gaertn commonly is used as aphrodisiac herb. Phytosterol, saponin, flavonoid and tannin of the herb have a certain bioactivity and may affect to the body system. The objective of this research was to examine the antifertility effects of sam jawa (Talinum paniculatum Gaertn. root extract (SJRE on male mice (Mus musculus L.. Twenty male mice were divided into 4 groups randomly with 5 replications. SJRE was dissolved in aquadest and given orally everyday for 34 days. The treatment dosages were 0 (control, 100,200, and 300 mg/kg BW. At 35th day mice were sacrificed and sectioned to remove testes and epididymis spermatozoas. Testes were sectioned using paraffin method and stained using Haematoxyllin-Eosin. Spermatogenic cells in each seminiferous tubule were counted to investigated spermatogenesis activity of testes. Epididymis sperm suspension was used to investigate sperm quality i.e: morphology, velocity and motility. Quantitatives data were analized using ANOVA and continued DMRT on 5% significance level. The result showed SJRE had antifertility effects on male mice (Mus musculus L. could inhibit spermatogenesis (decrease the spermatogenic cells count and decrease the sperm quality (increase percentage of abnormal sperm, decrease sperm motility and also decrease sperm velocity.

  8. Molecular heterogeneity in major urinary proteins of Mus musculus subspecies: potential candidates involved in speciation

    Science.gov (United States)

    Hurst, Jane L.; Beynon, Robert J.; Armstrong, Stuart D.; Davidson, Amanda J.; Roberts, Sarah A.; Gómez-Baena, Guadalupe; Smadja, Carole M.; Ganem, Guila

    2017-01-01

    When hybridisation carries a cost, natural selection is predicted to favour evolution of traits that allow assortative mating (reinforcement). Incipient speciation between the two European house mouse subspecies, Mus musculus domesticus and M.m.musculus, sharing a hybrid zone, provides an opportunity to understand evolution of assortative mating at a molecular level. Mouse urine odours allow subspecific mate discrimination, with assortative preferences evident in the hybrid zone but not in allopatry. Here we assess the potential of MUPs (major urinary proteins) as candidates for signal divergence by comparing MUP expression in urine samples from the Danish hybrid zone border (contact) and from allopatric populations. Mass spectrometric characterisation identified novel MUPs in both subspecies involving mostly new combinations of amino acid changes previously observed in M.m.domesticus. The subspecies expressed distinct MUP signatures, with most MUPs expressed by only one subspecies. Expression of at least eight MUPs showed significant subspecies divergence both in allopatry and contact zone. Another seven MUPs showed divergence in expression between the subspecies only in the contact zone, consistent with divergence by reinforcement. These proteins are candidates for the semiochemical barrier to hybridisation, providing an opportunity to characterise the nature and evolution of a putative species recognition signal. PMID:28337988

  9. Multiple origins of the phenol reaction negative phenotype in foxtail millet, Setaria italica (L.) P. Beauv., were caused by independent loss-of-function mutations of the polyphenol oxidase (Si7PPO) gene during domestication.

    Science.gov (United States)

    Inoue, Takahiko; Yuo, Takahisa; Ohta, Takeshi; Hitomi, Eriko; Ichitani, Katsuyuki; Kawase, Makoto; Taketa, Shin; Fukunaga, Kenji

    2015-08-01

    Foxtail millet shows variation in positive phenol color reaction (Phr) and negative Phr in grains, but predominant accessions of this crop are negative reaction type, and the molecular genetic basis of the Phr reaction remains unresolved. In this article, we isolated polyphenol oxidase (PPO) gene responsible for Phr using genome sequence information and investigated molecular genetic basis of negative Phr and crop evolution of foxtail millet. First of all, we searched for PPO gene homologs in a foxtail millet genome database using a rice PPO gene as a query and successfully found three copies of the PPO gene. One of the PPO gene homologs on chromosome 7 showed the highest similarity with PPO genes expressed in hulls (grains) of other cereal species including rice, wheat, and barley and was designated as Si7PPO. Phr phenotypes and Si7PPO genotypes completely co-segregated in a segregating population. We also analyzed the genetic variation conferring negative Phr reaction. Of 480 accessions of the landraces investigated, 87 (18.1 %) showed positive Phr and 393 (81.9 %) showed negative Phr. In the 393 Phr negative accessions, three types of loss-of-function Si7PPO gene were predominant and independently found in various locations. One of them has an SNP in exon 1 resulting in a premature stop codon and was designated as stop codon type, another has an insertion of a transposon (Si7PPO-TE1) in intron 2 and was designated as TE1-insertion type, and the other has a 6-bp duplication in exon 3 resulting in the duplication of 2 amino acids and was designated as 6-bp duplication type. As a rare variant of the stop codon type, one accession additionally has an insertion of a transposon, Si7PPO-TE2, in intron 2 and was designated as "stop codon +TE2 insertion type". The geographical distribution of accessions with positive Phr and those with three major types of negative Phr was also investigated. Accessions with positive Phr were found in subtropical and tropical regions at

  10. NCBI nr-aa BLAST: CBRC-STRI-01-2632 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2632 ref|NP_076136.2| non-imprinted in Prader-Willi/Angelman syndrome ...2 [Mus musculus] sp|Q9JJC8|NIPA2_MOUSE RecName: Full=Magnesium transporter NIPA2; AltName: Full=Non-imprinte...tein product [Mus musculus] tpg|DAA01178.1| TPA_exp: non-imprinted in Prader-Willi/Angelman syndrome 2 [Mus ...musculus] dbj|BAE32806.1| unnamed protein product [Mus musculus] gb|EDL21872.1| non imprint...ed in Prader-Willi/Angelman syndrome 2 homolog (human), isoform CRA_a [Mus musculus] gb|EDL21874.1| non imprint

  11. A qualitative study of how patients with type 2 diabetes use an electronic stand-alone personal health record.

    Science.gov (United States)

    Fuji, Kevin T; Abbott, Amy A; Galt, Kimberly A

    2015-04-01

    Patient use of personal health records (PHRs) to manage their health information has been proposed to enhance patient knowledge and empower patients to make changes in their self-care behaviors. However, there remains a gap in understanding about patients' actual PHR use behaviors. The purpose of this qualitative study was to explore how patients with type 2 diabetes used a PHR to manage their diabetes-related health information for self-care. Fifty-nine patients with type 2 diabetes were interviewed 3-6 months after receiving initial training on a free-of-charge, Web-based PHR. Interviews were audio-recorded, transcribed, and analyzed using an iterative process of in vivo coding, categorization, and theme development. Nine themes emerged, three of which expressed positive experiences: complete and accessible record; increased awareness; and behavioral changes. The remaining six themes expressed negative experiences: out of sight, out of mind; I would have used it if I were sicker; economic, infrastructure, and computer literacy barriers; lack of patient-provider engagement; double tracking; and privacy and security concerns. Despite some potential positive benefits resulting from PHR use, several barriers inhibited sustained and effective use over time. Provider and patient education about the benefits of PHR use and about the potential for filling in information gaps in the provider-based record is key to engage patients and stimulate PHR adoption and use.

  12. NCBI nr-aa BLAST: CBRC-STRI-01-1937 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-1937 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9E...P51|VN1B1_MOUSE RecName: Full=Vomeronasal type-1 receptor B1; AltName: Full=Vomeronasal type-1 receptor A5; AltName: Full=Vomeronasa...l receptor 2; AltName: Full=Pheromone receptor VN2 gb|AAG42083.1|AF291489_1 vomeronasa...l receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 [Mus musculus] gb|AAI07184.1| Vomeronasa...l 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 2e-25 41% ...

  13. Dicty_cDB: Contig-U12567-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available S1) RecName: Full=Protein lava lamp; &AE014298_609(AE01429... 36 4.2 EU708920_1( EU708920 |pid:none) Canine rotavirus...none) Mus musculus B16 F10Y cells cDNA, ... 35 7.1 DQ005106_1( DQ005106 |pid:none) Human rotavirus... Full=Myosin-9; AltName: Full=Myosin heavy chai... 35 7.1 AY787649_1( AY787649 |pid:none) Human rotavirus A ...7211_1( AK147211 |pid:none) Mus musculus 15 days embryo liver ... 35 7.1 EF554090_1( EF554090 |pid:none) Human rotavirus...rotein 3; Short=... 35 7.1 EF672579_1( EF672579 |pid:none) Human rotavirus A strain DS-1 non-... 35 7.1 ( Q6

  14. The uncharacterized gene 1700093K21Rik and flanking regions are correlated with reproductive isolation in the house mouse, Mus musculus.

    Science.gov (United States)

    Kass, David H; Janoušek, Václav; Wang, Liuyang; Tucker, Priscilla K

    2014-06-01

    Reproductive barriers exist between the house mouse subspecies, Mus musculus musculus and M. m. domesticus, members of the Mus musculus species complex, primarily as a result of hybrid male infertility, and a hybrid zone exists where their ranges intersect in Europe. Using single nucleotide polymorphisms (SNPs) diagnostic for the two taxa, the extent of introgression across the genome was previously compared in these hybrid populations. Sixty-nine of 1316 autosomal SNPs exhibited reduced introgression in two hybrid zone transects suggesting maladaptive interactions among certain loci. One of these markers is within a region on chromosome 11 that, in other studies, has been associated with hybrid male sterility of these subspecies. We assessed sequence variation in a 20 Mb region on chromosome 11 flanking this marker, and observed its inclusion within a roughly 150 kb stretch of DNA showing elevated sequence differentiation between the two subspecies. Four genes are associated with this genomic subregion, with two entirely encompassed. One of the two genes, the uncharacterized 1700093K21Rik gene, displays distinguishing features consistent with a potential role in reproductive isolation between these subspecies. Along with its expression specifically within spermatogenic cells, we present various sequence analyses that demonstrate a high rate of molecular evolution of this gene, as well as identify a subspecies amino acid variant resulting in a structural difference. Taken together, the data suggest a role for this gene in reproductive isolation.

  15. Genome patterns of selection and introgression of haplotypes in natural populations of the house mouse (Mus musculus.

    Directory of Open Access Journals (Sweden)

    Fabian Staubach

    Full Text Available General parameters of selection, such as the frequency and strength of positive selection in natural populations or the role of introgression, are still insufficiently understood. The house mouse (Mus musculus is a particularly well-suited model system to approach such questions, since it has a defined history of splits into subspecies and populations and since extensive genome information is available. We have used high-density single-nucleotide polymorphism (SNP typing arrays to assess genomic patterns of positive selection and introgression of alleles in two natural populations of each of the subspecies M. m. domesticus and M. m. musculus. Applying different statistical procedures, we find a large number of regions subject to apparent selective sweeps, indicating frequent positive selection on rare alleles or novel mutations. Genes in the regions include well-studied imprinted loci (e.g. Plagl1/Zac1, homologues of human genes involved in adaptations (e.g. alpha-amylase genes or in genetic diseases (e.g. Huntingtin and Parkin. Haplotype matching between the two subspecies reveals a large number of haplotypes that show patterns of introgression from specific populations of the respective other subspecies, with at least 10% of the genome being affected by partial or full introgression. Using neutral simulations for comparison, we find that the size and the fraction of introgressed haplotypes are not compatible with a pure migration or incomplete lineage sorting model. Hence, it appears that introgressed haplotypes can rise in frequency due to positive selection and thus can contribute to the adaptive genomic landscape of natural populations. Our data support the notion that natural genomes are subject to complex adaptive processes, including the introgression of haplotypes from other differentiated populations or species at a larger scale than previously assumed for animals. This implies that some of the admixture found in inbred strains of mice

  16. NCBI nr-aa BLAST: CBRC-STRI-01-2305 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2305 ref|NP_444455.1| vomeronasal 1 receptor, B1 [Mus musculus] sp|Q9E...P51|VN1B1_MOUSE RecName: Full=Vomeronasal type-1 receptor B1; AltName: Full=Vomeronasal type-1 receptor A5; AltName: Full=Vomeronasa...l receptor 2; AltName: Full=Pheromone receptor VN2 gb|AAG42083.1|AF291489_1 vomeronasa...l receptor V1RB1 [Mus musculus] gb|AAG43248.1| VN2 [Mus musculus] gb|AAI07184.1| Vomeronasa...l 1 receptor, B1 [Mus musculus] gb|EDK99276.1| vomeronasal 1 receptor, B1 [Mus musculus] NP_444455.1 8e-64 55% ...

  17. Gene : CBRC-MMUS-06-0104 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0104 6 A Pheromone receptors V1A16_RAT 2e-53 39% ref|NP_598931.1| vomeronasa...l 1 receptor, C15 [Mus musculus] gb|AAL47876.1| vomeronasal receptor V1RC15 [Mus musculus] dbj|BAC2634...7.1| unnamed protein product [Mus musculus] gb|AAI19598.1| Vomeronasal 1 receptor, C15 [Mus musculus] gb|AAI19597.1| Vomeronasa...l 1 receptor, C15 [Mus musculus] gb|EDK98787.1| vomeronasal 1 ...l-length enriched library, clone:4831417D05 product:similar to VOMERONASAL RECEPTOR V1RC3 [Mus musculus], fu

  18. NCBI nr-aa BLAST: CBRC-MMUS-07-0085 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-07-0085 ref|NP_665841.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAM...69674.1|AF394954_1 vomeronasal receptor 1 E2 [Mus musculus] dbj|BAB79222.1| vomeronasal receptor 1 E6 [Mus m...usculus] gb|AAM62399.1| vomeronasal receptor V1RE9 [Mus musculus] gb|AAI41483.1| Vomeronasal 1 receptor, E9 ...[synthetic construct] gb|EDL03620.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAI48767.1| Vomeronasa

  19. NCBI nr-aa BLAST: CBRC-RNOR-01-0219 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-01-0219 ref|NP_665841.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAM...69674.1|AF394954_1 vomeronasal receptor 1 E2 [Mus musculus] dbj|BAB79222.1| vomeronasal receptor 1 E6 [Mus m...usculus] gb|AAM62399.1| vomeronasal receptor V1RE9 [Mus musculus] gb|AAI41483.1| Vomeronasal 1 receptor, E9 ...[synthetic construct] gb|EDL03620.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAI48767.1| Vomeronasa

  20. NCBI nr-aa BLAST: CBRC-RNOR-01-0216 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-01-0216 ref|NP_665841.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAM...69674.1|AF394954_1 vomeronasal receptor 1 E2 [Mus musculus] dbj|BAB79222.1| vomeronasal receptor 1 E6 [Mus m...usculus] gb|AAM62399.1| vomeronasal receptor V1RE9 [Mus musculus] gb|AAI41483.1| Vomeronasal 1 receptor, E9 ...[synthetic construct] gb|EDL03620.1| vomeronasal 1 receptor, E9 [Mus musculus] gb|AAI48767.1| Vomeronasa

  1. Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.

    Science.gov (United States)

    Sanford, J; Forrester, L; Chapman, V; Chandley, A; Hastie, N

    1984-03-26

    The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic DNA methylation between sperm and oocyte DNA. The methylation levels of the minor satellite sequences did not change during spermiogenesis, and were not associated with the onset of meiosis or a specific stage in sperm development.

  2. Tissue-Specific Contributions of Paternally Expressed Gene 3 in Lactation and Maternal Care of Mus musculus.

    Directory of Open Access Journals (Sweden)

    Wesley D Frey

    Full Text Available Paternally Expressed Gene 3 (Peg3 is an imprinted gene that controls milk letdown and maternal-caring behaviors. In this study, a conditional knockout allele has been developed in Mus musculus to further characterize these known functions of Peg3 in a tissue-specific manner. The mutant line was first crossed with a germline Cre. The progeny of this cross displayed growth retardation phenotypes. This is consistent with those seen in the previous mutant lines of Peg3, confirming the usefulness of the new mutant allele. The mutant line was subsequently crossed individually with MMTV- and Nkx2.1-Cre lines to test Peg3's roles in the mammary gland and hypothalamus, respectively. According to the results, the milk letdown process was impaired in the nursing females with the Peg3 mutation in the mammary gland, but not in the hypothalamus. This suggests that Peg3's roles in the milk letdown process are more critical in the mammary gland than in the hypothalamus. In contrast, one of the maternal-caring behaviors, nest-building, was interrupted in the females with the mutation in both MMTV- and Nkx2.1-driven lines. Overall, this is the first study to introduce a conditional knockout allele of Peg3 and to further dissect its contribution to mammalian reproduction in a tissue-specific manner.

  3. Serine/threonine-protein phosphatase 1 α levels are paralleling olfactory memory formation in the CD1 mouse.

    Science.gov (United States)

    Winding, Christiana; Sun, Yanwei; Höger, Harald; Bubna-Littitz, Hermann; Pollak, Arnold; Schmidt, Peter; Lubec, Gert

    2011-06-01

    Although olfactory discrimination has already been studied in several mouse strains, data on protein levels linked to olfactory memory are limited. Wild mouse strains Mus musculus musculus, Mus musculus domesticus and CD1 laboratory outbred mice were tested in a conditioned odor preference task and trained to discriminate between two odors, Rose and Lemon, by pairing one odor with a sugar reward. Six hours following the final test, mice were sacrificed and olfactory bulbs (OB) were taken for gel-based proteomics analyses and immunoblotting. OB proteins were extracted, separated by 2-DE and quantified using specific software (Proteomweaver). Odor-trained mice showed a preference for the previously rewarded odor suggesting that conditioned odor preference occurred. In CD1 mice levels, one out of 482 protein spots was significantly increased in odor-trained mice as compared with the control group; it was in-gel digested by trypsin and chymotrypsin and analyzed by tandem mass spectrometry (nano-ESI-LC-MS/MS). The spot was unambiguously identified as serine/threonine-protein phosphatase PP1-α catalytic subunit (PP-1A) and differential levels observed in gel-based proteomic studies were verified by immunoblotting. PP-1A is a key signalling element in synaptic plasticity and memory processes and is herein shown to be paralleling olfactory discrimination representing olfactory memory. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Development of polysaccharide based biodegradable packaging materials suitable for radiation sterilization

    International Nuclear Information System (INIS)

    Yasin, Tariq

    2011-01-01

    The biodegradable composites are prepared by melt blending method in Poly-lab Rheomix internal mixer from Thermo Electron. The high-density polyethylene (90 wt%) and maleic anhydride grafted polyethylene (10 wt%) are heat melted at 170 °C under the constant rotors speed (60 rpm). After 3 min, maize starch (Gelose 80) 10 parts per hundred (phr), sepiolite, stearic acid (1 phr), Irganox 1010 (0.2 phr) and Irgafos 168 (0.1 phr) are added and mixed for another 15 min. The sepiolite is varied from 2 to 6 phr in these composites. The PE0, PE2, PE4 and PE6 identification codes are used to represent composites containing 0, 2, 4 and 6 phr of sepiolite respectively. The heat pressed sheets are prepared at 170 οC at 200 bar. These sheet are irradiated under electron beam in air at room temperature using UELV-10-10S linear electron beam irradiator (NIIEFA, Russia) at 10 MeV and 1mA using 1 kGy/pass. The samples are subjected to various doses of electron beam irradiation ranging from 25 to 100 kGy. The structural and morphological analysis of developed composites is characterized by Fourier transform infrared spectrophotometer and scanning electron microscope. The thermal properties are investigated using thermo-gravimetric analyzer and differential scanning calorimeter. The gel content and tensile properties are also measured

  5. Development of polysaccharide based biodegradable packaging materials suitable for radiation sterilization

    Energy Technology Data Exchange (ETDEWEB)

    Yasin, Tariq [Department of Metallurgy and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore, Islamabad (Pakistan)

    2011-07-01

    The biodegradable composites are prepared by melt blending method in Poly-lab Rheomix internal mixer from Thermo Electron. The high-density polyethylene (90 wt%) and maleic anhydride grafted polyethylene (10 wt%) are heat melted at 170 °C under the constant rotors speed (60 rpm). After 3 min, maize starch (Gelose 80) 10 parts per hundred (phr), sepiolite, stearic acid (1 phr), Irganox 1010 (0.2 phr) and Irgafos 168 (0.1 phr) are added and mixed for another 15 min. The sepiolite is varied from 2 to 6 phr in these composites. The PE0, PE2, PE4 and PE6 identification codes are used to represent composites containing 0, 2, 4 and 6 phr of sepiolite respectively. The heat pressed sheets are prepared at 170 οC at 200 bar. These sheet are irradiated under electron beam in air at room temperature using UELV-10-10S linear electron beam irradiator (NIIEFA, Russia) at 10 MeV and 1mA using 1 kGy/pass. The samples are subjected to various doses of electron beam irradiation ranging from 25 to 100 kGy. The structural and morphological analysis of developed composites is characterized by Fourier transform infrared spectrophotometer and scanning electron microscope. The thermal properties are investigated using thermo-gravimetric analyzer and differential scanning calorimeter. The gel content and tensile properties are also measured.

  6. Main: 1U3C [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available olecule: Cryptochrome 1 Apoprotein; Chain: A; Fragment: Phr Domain, Residues 1-509; Synonym: Blue Light Phot....Machius, J.Deisenhofer Structure Of The Photolyase-Like Domain Of Cryptochrome 1 From Arabidopsis Thaliana.

  7. Main: 1U3D [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available olecule: Cryptochrome 1 Apoprotein; Chain: A; Fragment: Phr Domain, Residues 1-509; Synonym: Blue Light Phot....Machius, J.Deisenhofer Structure Of The Photolyase-Like Domain Of Cryptochrome 1 From Arabidopsis Thaliana.

  8. Quality characteristics of the Musculus longissimus dorsi from Pecora dell’Amiata reared in Tuscany

    Directory of Open Access Journals (Sweden)

    Roberto Tocci

    2017-07-01

    The trial was performed with ewes and lambs deriving from the local breed Pecora dell’Amiata. In this work, the Musculus longissimus dorsi (M. longissimus thoracis + lomborum physical-chemical and nutritional characteristics of 23 ewes and 20 lambs were compared. The ewes of the trial were over 7 years old while the lambs were on average 80 days old. Ewe meat has shown lower drip loss (4.14 vs 2.71% and lightness (L* 38.6 vs 45.3 values, and higher PH (6.15±0.07, shear force (8.4 vs 2.31 kg, fat content (5.9 vs 2.0%. The lamb meat lipids had higher polyunsaturated fatty acid content (PUFA: 14.58 vs 9.25% and higher PUFA/Saturated Fatty Acids (SFA ratio (0.31 vs 0.20. The PCA analysis identified two distinct groups regarding ewe and lamb meat respectively for the fatty acids composition and the health indices. Ewe meat showed dietetic and nutritional characteristics similar to that of lamb meat. These characteristics may allow in the future, to the ewe meat valorisation, now not appreciated by Tuscan and Italian market.

  9. Efficiency of 1,9- Nonane-diol-diacrylate as a radiation vulcanization accelerator for natural rubber latex

    International Nuclear Information System (INIS)

    Haque, M.E.

    2003-09-01

    The efficiency of 1,9- Nonane-diol-diacrylate (NDDA) as a radiation vulcanization accelerator (RVA) for natural rubber latex (NRL) was investigated. Both gamma rays and electron beam (EB) were used for vulcanizing NRL with NDDA. The radiation dose of gamma rays, concentration of NDDA required to vulcanize the latex were optimized. 20 kGy radiation dose of gamma rays and 5 phr concentration of NDDA were found optimum to get maximum tensile and related properties. NRL was vulcanized under EB to find out the optimum condition of irradiation with this RVA. The defoamer concentrations, length of irradiation time under EB, concentration of RVA were optimizer by changing various parameters of the EB machine with a constant set of the others. 0.2 phr defoamer concentration, 30 minutes irradiation time and 5 phr concentration of NDDA were found optimum for irradiation of NRL under the EB machine. Effect of low current and optimum volume of latex charged in the reaction vessel suitable for vulcanization at a time were found out. (author)

  10. Dicty_cDB: Contig-U03036-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available DQ379469 ) Concholepas concholepas microsatellite Cc1H2 sequ... 50 0.17 1 ( AF10...e DKEY-65O14 in l... 50 0.17 1 ( AC118018 ) Mus musculus chromosome 17, clone RP23-458D20, co... 50 0.17 1 (

  11. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA

    OpenAIRE

    Schertzer , Michael; Jouravleva , Karina; Perderiset , Mylène; Dingli , Florent; Loew , Damarys; Le Guen , Tangui; Bardoni , Barbara; De Villartay , Jean-Pierre; Revy , Patrick; Londono-Vallejo , Arturo

    2015-01-01

    International audience; Hoyeraal-Hreidarsson syndrome (HHS) is a severe form of Dyskeratosis congenita characterized by developmental defects, bone marrow failure and im-munodeficiency and has been associated with telom-ere dysfunction. Recently, mutations in Regulator of Telomere ELongation helicase 1 (RTEL1), a helicase first identified in Mus musculus as being responsible for the maintenance of long telomeres, have been identified in several HHS patients. Here we show that RTEL1 is require...

  12. A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Regla Bustos

    2010-09-01

    Full Text Available Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF has an important role in the control of phosphate (Pi starvation stress responses. Using transcriptomic analysis of Pi starvation in phr1, and phr1 phr1-like (phl1 mutants and in wild type plants, we show that PHR1 in conjunction with PHL1 controls most transcriptional activation and repression responses to phosphate starvation, regardless of the Pi starvation specificity of these responses. Induced genes are enriched in PHR1 binding sequences (P1BS in their promoters, whereas repressed genes do not show such enrichment, suggesting that PHR1(-like control of transcriptional repression responses is indirect. In agreement with this, transcriptomic analysis of a transgenic plant expressing PHR1 fused to the hormone ligand domain of the glucocorticoid receptor showed that PHR1 direct targets (i.e., displaying altered expression after GR:PHR1 activation by dexamethasone in the presence of cycloheximide corresponded largely to Pi starvation-induced genes that are highly enriched in P1BS. A minimal promoter containing a multimerised P1BS recapitulates Pi starvation-specific responsiveness. Likewise, mutation of P1BS in the promoter of two Pi starvation-responsive genes impaired their responsiveness to Pi starvation, but not to other stress types. Phylogenetic footprinting confirmed the importance of P1BS and PHR1 in Pi starvation responsiveness and indicated that P1BS acts in concert with other cis motifs. All together, our data show that PHR1 and PHL1 are partially redundant TF acting as central integrators of Pi starvation responses, both specific and generic. In addition, they indicate that transcriptional repression responses are an integral part of adaptive responses to stress.

  13. New Metacentric Populations and Phylogenetic Hypotheses Involving Whole-Arm Reciprocal Translocation in Mus musculus domesticus from Sicily, Southern Italy.

    Science.gov (United States)

    Castiglia, Riccardo; Capanna, Ernesto; Bezerra, Alexandra M R; Bizzoco, Domenico; Zambigli, Emanuela; Solano, Emanuela

    2015-01-01

    The house mouse Mus musculus domesticus is characterized by more than 100 metacentric populations, due to the occurrence of Robertsonian (Rb) fusions, together with the standard all-telocentric karyotype (2n = 40). We examined G-banded karyotypes of 18 mice from 10 localities in Sicily and describe 3 new metacentric populations: 'Ragusa Ibla' (IRAG), 2n = 33-36, Rb(2.4), Rb(5.6), Rb(9.16), Rb(13.17); 'Piana degli Albanesi' (IPIA), 2n = 23, Rb(1.18), Rb(2.15), Rb(3.5), Rb(4.12), Rb(6.11), Rb(7.8), Rb(9.16), Rb(10.14), Rb(13.17); 'Trapani' (ITRA), 2n = 22, Rb(1.18), Rb(2.15), Rb(3.7), Rb(4.12), Rb(5.9), Rb(6.11), Rb(8.16), Rb(10.14), Rb(13.17). Three mice belonged to the previously reported 'Castelbuono' race (ICAS), 2n = 24, which is very similar to the nearby 'Palermo' (IPAL) race, 2n = 26. Three Rb fusions not yet observed in wild mouse populations were identified: Rb(3.5), Rb(3.7) and Rb(5.9). Rb fusions shared among 4 races (IPIA, IRAG, ICAS, and IPAL) allowed us to describe their potential phylogenetic relationships. We obtained 2 alternative phylogenetic trees. The differences between them are mainly due to various modes of formation of IPIA and ITRA. In the first hypothesis, the specific Rb fusions occurred independently. In the second, those of IRAG originated from those of IPIA via whole-arm reciprocal translocations. © 2015 S. Karger AG, Basel.

  14. Ultrastructural characters of the spermatozoa in Digeneans of the genus Lecithochirium Lühe, 1901 (Digenea, Hemiuridae, parasites of fishes: comparative study of L. microstomum and L. musculus

    Directory of Open Access Journals (Sweden)

    Ndiaye Papa Ibnou

    2014-01-01

    Full Text Available This study provides the first ultrastructural data of spermatozoa in the genus Lecithochirium. The spermatozoa of L. microstomum (from Trichiurus lepturus in Senegal and L. musculus (from Anguilla anguilla in Corsica exhibit the general pattern described in the great majority of the Digenea, namely two axonemes with the 9 + “1” pattern typical of the Trepaxonemata, one mitochondrion, a nucleus, parallel cortical microtubules and external ornamentation of the plasma membrane. Spermatozoa of L. microstomum and L. musculus have some specific features such as the presence of a reduced number of cortical microtubules arranged on only one side of the spermatozoon, the lack of spine-like bodies and expansion of the plasma membrane. The external ornamentation of the plasma membrane entirely covers the anterior extremity of the spermatozoa. The ultrastructure of the posterior extremity of the spermatozoa corresponds to the pattern previously described in the Hemiuridae, characterized by only singlets of the second axoneme. A particularity of these spermatozoa is the organization of the microtubule doublets of the second axoneme around the nucleus in the posterior part of the spermatozoon.

  15. Male meiosis and gametogenesis in wild house mice (Mus musculus domesticus) from a chromosomal hybrid zone; a comparison between "simple" Robertsonian heterozygotes and homozygotes.

    Science.gov (United States)

    Wallace, B M; Searle, J B; Everett, C A

    1992-01-01

    Wild male house mice Mus musculus domesticus were collected from the hybrid zone between the John o'Groats race (2n = 32) and the standard race (2n = 40) in northern Scotland. Meiosis in both homozygotes (2n = 32, 36, and 40) and single Robertsonian heterozygotes (2n = 33, 35, and 37) was found to be orderly. At prophase/metaphase I in heterozygotes, a trivalent was formed from the metacentric and two homologous acrocentrics. At pachytene, this trivalent usually had a single side arm at the position of the centromeres, as a result of nonhomologous pairing of the acrocentrics. This side arm persisted into diplotene. Generally only a single chiasma was formed between each acrocentric and the metacentric. Anaphase I nondisjunction frequencies were estimated as 1.5% for the homozygotes and 2.7% for the heterozygotes. The extent of germ cell death between the pachytene and round spermatid stages was 18% greater in heterozygotes than in homozygotes. Our results concur with previous studies which indicate that single Robertsonian heterozygotes in wild house mice have near-normal fertility.

  16. Efecto del extracto del fruto de Physalis peruviana "tomatillo" en Mus musculus var. swis con hiperlipidemia inducida.

    Directory of Open Access Journals (Sweden)

    Julio Campos Florián

    2011-01-01

    Full Text Available El objetivo de la presente i nvestigación fue determinar la actividad hipolipidémica del fruto de Physalis peruviana “tomatillo” en un modelo de hiperlipidemia aguda inducida con tritón. Se utilizaron Mus musculus var. swis machos como animales de experimentación. Se trabajó con cuatro grupos de ratones, el grupo blanco recibió agua destilada por vía oral y solución salina fisiológica por vía intraperitoneal, el grupo control recibió agua destilada por vía oral y tritón por vía intraperitoneal, el grupo problema 1 recibió por vía oral 0.05g/100g del extracto de Physalis peruviana y tritón por vía intraperitoneal y el grupo problema 2 recibió por vía oral 0.2g/100g del extracto de Physalis peruviana y tritón por vía intraperitoneal. Luego de 24 horas de administrar los tratamientos se re alizaron las mediciones en suero de las concentraciones de colesterol y triglicéridos. Los niveles promedio de colesterol (mg/dL fueron: 58.87±11.54 (blanco, 121.71±15.00 (control, 58.08±9. 21 (problema 1 y 66.78±16.77 (problema 2. Los niveles promedio de triglicéridos (g/L fueron: 0.48±0.07 (blanco, 1.84±0.18 (control, 0.34±0.10 (problema 1 y 0.94±0.25 (problema 2. Se encontró reducciones significativas (p<0.000, tanto de las concentraciones de colesterol como de triglicéridos en relación a las o btenidas en el grupo tratado sólo con tritón.

  17. Evaluating the functional outcomes of ultrasound-guided botulinum toxin type A injections using the Euro-musculus approach for upper limb spasticity treatment in post-stroke patients; an observational study.

    Science.gov (United States)

    Buyukavci, Raikan; Akturk, Semra; Ersoy, Yüksel

    2018-02-07

    Ultrasound-guided botulinum toxin type A injection is an effective treatment for spasticity. Euro-musculus spasticity approach is a new method for administering injections to the correct point of the correct muscle. The clinical outcomes of this practical approach is not yet available in the literature. The purpose of this study was to evaluate the effects on spasticity and the functional outcomes of ultrasound guided botulinum toxin type A injections via the Euro-musculus spasticity approach to treat upper limb spasticity in post-stroke patients. An observational study. Inpatient post-stroke patients. Twenty five post-stroke patients with post-stroke upper limb spasticity were recruited. The ultrasound-guided botulinum toxin type A injections were administered into the spastic target muscles using the Euro-musculus spasticity approach, and all of the patients were enrolled in rehabilitation programmes after the injections. This research included the innervation zone and injection site figures and ultrasound images of each muscle in the upper limb. The degree of spasticity was assessed via the Modified Ashworth Scale and the upper limb motor function via the Fugl Meyer Upper Extremity Scale at the baseline and 4 and 12 weeks after the botulinum toxin type A injection. Significant decreases in the Modified Ashworth Scale scores of the upper limb flexor muscle tone measured 4 and 12 weeks after the botulinum toxin type A injection were found when compared to the baseline scores (pbotulinum toxin type A injection via the Euro- musculus spasticity approach is a practical and effective method for administering injections to the correct point of the correct muscle. Ultrasound-guided botulinum toxin type A injections combined with rehabilitation programmes decrease spasticity and improve the upper extremity motor functions in stroke patients. This new approach for ultrasound- guided botulinum toxin type A injection is very practical and effective method for upper

  18. Dicty_cDB: Contig-U11532-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cology Modeling. 62 6e-05 1 ( AC004912 ) Homo sapiens PAC clone RP5-871B15 from 7, ...n chromo... 62 6e-05 1 ( AC122028 ) Mus musculus BAC clone RP24-394D4 from 2, complet... 62 6e-05 1 ( DD267290 ) Molecular Hepatotoxi

  19. Pengaruh sulfur terhadap sifat fisika campuran pale crepe dan SBR untuk karet tahan panas

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2013-12-01

    Full Text Available Sulfur plays an important role in the rubber vulcanization process especially in the formation of crosslinks. Heat resistant rubber made from mixing pale crepe and SBR requires the right amount of sulfur as crosslinking agent. The purpose of the study was to determine the effect of the addition of sulfur on the changes in physical properties before and after aging. Heat resistant rubber was made with variation of pale crepe/SBR: 80/20; 70/30; 60/40; 50/50 phr and sulfur variation of 1; 1.5 phr. The results showed that sulfur was influential in the crosslinks formation. The addition of 1 phr sulfur gave higher physical properties of the vulcanized with 1.5 phr sulfur. The changes of physical properties after aging process of the vulcanized with sulfur 1 phr was lower than the vulcanized with sulfur 1.5 phr. Vulcanized pale crepe/SBR (70/30 with 1 phr sulfur could be applied as heat-resistant rubber products.

  20. Effect of eccentric exercise with reduced muscle glycogen on plasma interleukin-6 and neuromuscular responses of musculus quadriceps femoris.

    Science.gov (United States)

    Gavin, James P; Myers, Stephen D; Willems, Mark E T

    2016-07-01

    Eccentric exercise can result in muscle damage and interleukin-6 (IL-6) secretion. Glycogen availability is a potent stimulator of IL-6 secretion. We examined effects of eccentric exercise in a low-glycogen state on neuromuscular function and plasma IL-6 secretion. Twelve active men (23 ± 4 yr, 179 ± 5 cm, 77 ± 10 kg, means ± SD) completed two downhill treadmill runs (gradient, -12%, 5 × 8 min; speed, 12.1 ± 1.1 km/h) with normal (NG) and reduced muscle glycogen (RG) in randomized order and at least 6 wk apart. Muscle glycogen was reduced using an established cycling protocol until exhaustion and dietary manipulation the evening before the morning run. Physiological responses were measured up to 48 h after the downhill runs. During recovery, force deficits of musculus quadriceps femoris by maximal isometric contractions were similar. Changes in low-frequency fatigue were larger with RG. Voluntary activation and plasma IL-6 levels were similar in recovery between conditions. It is concluded that unaccustomed, damaging eccentric exercise with low muscle glycogen of the m. quadriceps femoris 1) exacerbated low-frequency fatigue but 2) had no additional effect on IL-6 secretion. Neuromuscular impairment after eccentric exercise with low muscle glycogen appears to have a greater peripheral component in early recovery. Copyright © 2016 the American Physiological Society.

  1. Effect of bovine pellucid zone 3 monoclonal antibodies on B cell lymphoma 2 expressions of granulosa cell and mice (Mus musculus follicle diameter

    Directory of Open Access Journals (Sweden)

    Heti Ira Ayue

    2017-01-01

    Full Text Available Objective: To evaluate the effects of pellucid zone 3 monoclonal antibodies against B-cell lymphoma 2 (BCL-2 expression and mice follicle diameter at various time periods.Methods: The animal model of this study was 36 Balb/c mice (Mus musculus. A true experimental design was used with a post-test only control group approach. BCL-2 expression was observed using immunohistochemistry, while the follicle diameter was observed by haematoxylin-eosin staining. The data was analyzed using nested ANOVA to compare the results of the mean expression of BCL-2 on the 5th and 20th day of observation in the pre-antral and antral follicle between the control and treatment groups.Results: No significant differences were found in BCL-2 gene expression. There were also no significant differences in BCL-2 expression on the 10th day of pre-antral follicle analysis. Moreover, there were no significant differences between the mean follicle diameter on the 5th, 10th, and 20th day of pre-antral and antral follicle development between the control and treatment groups. The addition of bovine pellucid zone 3 (bZP3 monoclonal antibodies on the 5th and 20th day of observation did not decrease the expression of BCL-2 gene in the pre-antral and antral follicle of mice. Administering bZP3 monoclonal antibodies on the 10th day of observation did not affect BCL-2 expression in the pre-antral follicle but did decrease BCL-2 expression in the antral follicle. Supplying bZP3 monoclonal antibodies on the 5th, 10th and 20th day did not affect the diameter of pre-antral and antral follicles of the mice.Conclusion: The monoclonal antibodies bovine zona pelusida 3 has the potential to be developed as a safe immunocontraception preparation.

  2. A note on the distribution and abundance of blue whales (Balaenoptera musculus in the Central and Northeast North Atlantic

    Directory of Open Access Journals (Sweden)

    Daniel G Pike

    2009-09-01

    Full Text Available The distribution and abundance of blue whales (Balaenoptera musculus was assessed from ship surveys conducted in the Central and Northeast Atlantic in 1987, 1989, 1995 and 2001. Blue whales were most commonly sighted off western Iceland, and to a lesser extent northeast of Iceland. They were very rare or absent in the Northeast Atlantic. Sightings were combined over all surveys to estimate the detection function using standard line transect methodology, with the addition of a covariate to account for differences between surveys. Total abundance was highest in 1995 (979, 95% CI 137-2,542 and lowest in 1987 (222, 95% CI 115-440. Uncertainty in species identity had little effect on estimates of abundance. There was a significant positive trend in abundance northeast of Iceland and in the total survey area.

  3. Syphacia obvelata (Nematode, Oxyuridae) infecting laboratory mice Mus musculus (Rodentia, Muridae): phylogeny and host-parasite relationship.

    Science.gov (United States)

    Abdel-Gaber, Rewaida

    2016-03-01

    Syphacia obvelata is a pinworm nematode parasite infecting man and laboratory animals in high abundance. This parasitological study was carried out during the period of March 2014-February 2015 to investigate the helminth parasites infecting the laboratory mice Mus musculus in the Animal House at Cairo University, Egypt. The prevalence of S. obvelata in M. musculus was 75.0 %. The extent of infection with S. obvelata is analyzed according to the sex of the host mice. It was shown that the prevalence of male infection was greater than female worms. Morphological characterization revealed that the present Oxyurid species possesses a rounded cephalic end with less developed lips, esophagus divided into cylindrical corpus, and globular bulb supported internally with valvular apparatus; three mamelons are located at the ventral surface with a single chitinized spicule and a gubernaculum provided with an accessory hook in males, and ovijector apparatus opens ventrally by the vulva surrounded by protruded lips in female worms. Body of the male was 0.623-1.130 (0.830 ± 0.11) mm long and 0.092-0.130 (0.110 ± 0.01) mm wide; the esophagus was 0.164-0.280 (0.210 ± 0.01) mm long; the nerve ring and excretory pore are located at 0.035-0.132 (0.073 ± 0.01) and 0.087-0.191 (0.145 ± 0.01) mm from the anterior end, respectively, while the female measured 2.930-4.650 (3.540 ± 0.1) mm long and 0.120-0.232 (0.156 ± 0.001) mm wide; the esophagus was 0.213-0.410 (0.342 ± 0.01) mm long; the nerve ring, excretory pore, and vulval opening are located at 0.026-0.157 (0.121 ± 0.01), 0.134-0.243 (0.195 ± 0.01), and 0.323-0.632 (0.546 ± 0.11) mm from the anterior end, respectively; eggs measured 0.120-0.139 (0.129 ± 0.001) mm long and 0.030-0.052 (0.045 ± 0.001) mm wide. It compared morphometrically with other Syphacia species described previously and showed little differences in

  4. Impact of an Electronic Health Record-Integrated Personal Health Record on Patient Participation in Health Care: Development and Randomized Controlled Trial of MyHealthKeeper.

    Science.gov (United States)

    Ryu, Borim; Kim, Nari; Heo, Eunyoung; Yoo, Sooyoung; Lee, Keehyuck; Hwang, Hee; Kim, Jeong-Whun; Kim, Yoojung; Lee, Joongseek; Jung, Se Young

    2017-12-07

    Personal health record (PHR)-based health care management systems can improve patient engagement and data-driven medical diagnosis in a clinical setting. The purpose of this study was (1) to demonstrate the development of an electronic health record (EHR)-tethered PHR app named MyHealthKeeper, which can retrieve data from a wearable device and deliver these data to a hospital EHR system, and (2) to study the effectiveness of a PHR data-driven clinical intervention with clinical trial results. To improve the conventional EHR-tethered PHR, we ascertained clinicians' unmet needs regarding PHR functionality and the data frequently used in the field through a cocreation workshop. We incorporated the requirements into the system design and architecture of the MyHealthKeeper PHR module. We constructed the app and validated the effectiveness of the PHR module by conducting a 4-week clinical trial. We used a commercially available activity tracker (Misfit) to collect individual physical activity data, and developed the MyHealthKeeper mobile phone app to record participants' patterns of daily food intake and activity logs. We randomly assigned 80 participants to either the PHR-based intervention group (n=51) or the control group (n=29). All of the study participants completed a paper-based survey, a laboratory test, a physical examination, and an opinion interview. During the 4-week study period, we collected health-related mobile data, and study participants visited the outpatient clinic twice and received PHR-based clinical diagnosis and recommendations. A total of 68 participants (44 in the intervention group and 24 in the control group) completed the study. The PHR intervention group showed significantly higher weight loss than the control group (mean 1.4 kg, 95% CI 0.9-1.9; Phealth tracker system and its potential to improve patient clinical profiles. ClinicalTrials.gov NCT03200119; https://clinicaltrials.gov/ct2/show/NCT03200119 (Archived by WebCite at http

  5. A Study of Personal Health Record User’s Behavioral Model Based on the PMT and UTAUT Integrative Perspective

    Directory of Open Access Journals (Sweden)

    Hui-Lung Hsieh

    2016-12-01

    Full Text Available The personal health record (PHR is a system that enables borderless medical care services by combining technological innovation and human consideration. This study explored factors affecting the adoption of PHR from technical, medical, and social perspectives according to the Protection Motivation Theory (PMT and Unified Theory of Acceptance and Use of Technology (UTAUT model. A survey using a structured questionnaire was subsequently conducted, which produced the following results: (1 The PMT and UTAUT were effective at predicting PHR usage behaviors; (2 Perceived ease-of-use was the most decisive factor influencing the use of PHR, followed by self-efficacy and perceived usefulness; and (3 Behavioral intention for PHR was significantly and positively correlated with usage behavior. From the obtained results, this study recommends that health authorities and medical institutions promote self-efficacy in the use of PHR to improve the levels of behavioral intention and usage behavior among the people. Additionally, medical care institutions are recommended to promote health management and preventive healthcare concepts to help improve public acceptance of the PHR system as a means to self-manage their health. Finally, community centers, medical institutions, and health authorities are urged to work together to enhance public medical knowledge and pool resources for the PHR system, both of which are essential for improving the popularity of the PHR, public quality of life, and the effectiveness of health management.

  6. A Study of Personal Health Record User's Behavioral Model Based on the PMT and UTAUT Integrative Perspective.

    Science.gov (United States)

    Hsieh, Hui-Lung; Kuo, Yu-Ming; Wang, Shiang-Ru; Chuang, Bi-Kun; Tsai, Chung-Hung

    2016-12-23

    The personal health record (PHR) is a system that enables borderless medical care services by combining technological innovation and human consideration. This study explored factors affecting the adoption of PHR from technical, medical, and social perspectives according to the Protection Motivation Theory (PMT) and Unified Theory of Acceptance and Use of Technology (UTAUT) model. A survey using a structured questionnaire was subsequently conducted, which produced the following results: (1) The PMT and UTAUT were effective at predicting PHR usage behaviors; (2) Perceived ease-of-use was the most decisive factor influencing the use of PHR, followed by self-efficacy and perceived usefulness; and (3) Behavioral intention for PHR was significantly and positively correlated with usage behavior. From the obtained results, this study recommends that health authorities and medical institutions promote self-efficacy in the use of PHR to improve the levels of behavioral intention and usage behavior among the people. Additionally, medical care institutions are recommended to promote health management and preventive healthcare concepts to help improve public acceptance of the PHR system as a means to self-manage their health. Finally, community centers, medical institutions, and health authorities are urged to work together to enhance public medical knowledge and pool resources for the PHR system, both of which are essential for improving the popularity of the PHR, public quality of life, and the effectiveness of health management.

  7. A Study of Personal Health Record User’s Behavioral Model Based on the PMT and UTAUT Integrative Perspective

    Science.gov (United States)

    Hsieh, Hui-Lung; Kuo, Yu-Ming; Wang, Shiang-Ru; Chuang, Bi-Kun; Tsai, Chung-Hung

    2016-01-01

    The personal health record (PHR) is a system that enables borderless medical care services by combining technological innovation and human consideration. This study explored factors affecting the adoption of PHR from technical, medical, and social perspectives according to the Protection Motivation Theory (PMT) and Unified Theory of Acceptance and Use of Technology (UTAUT) model. A survey using a structured questionnaire was subsequently conducted, which produced the following results: (1) The PMT and UTAUT were effective at predicting PHR usage behaviors; (2) Perceived ease-of-use was the most decisive factor influencing the use of PHR, followed by self-efficacy and perceived usefulness; and (3) Behavioral intention for PHR was significantly and positively correlated with usage behavior. From the obtained results, this study recommends that health authorities and medical institutions promote self-efficacy in the use of PHR to improve the levels of behavioral intention and usage behavior among the people. Additionally, medical care institutions are recommended to promote health management and preventive healthcare concepts to help improve public acceptance of the PHR system as a means to self-manage their health. Finally, community centers, medical institutions, and health authorities are urged to work together to enhance public medical knowledge and pool resources for the PHR system, both of which are essential for improving the popularity of the PHR, public quality of life, and the effectiveness of health management. PMID:28025557

  8. NCBI nr-aa BLAST: CBRC-STRI-01-2574 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2574 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 2e-85 52% ...

  9. NCBI nr-aa BLAST: CBRC-STRI-01-2691 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2691 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 1e-74 49% ...

  10. NCBI nr-aa BLAST: CBRC-STRI-01-2291 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2291 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 1e-79 50% ...

  11. NCBI nr-aa BLAST: CBRC-STRI-01-2568 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2568 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 5e-49 53% ...

  12. NCBI nr-aa BLAST: CBRC-STRI-01-0474 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-0474 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 7e-81 53% ...

  13. NCBI nr-aa BLAST: CBRC-STRI-01-0722 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-0722 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 7e-65 49% ...

  14. NCBI nr-aa BLAST: CBRC-STRI-01-2741 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2741 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 7e-83 53% ...

  15. NCBI nr-aa BLAST: CBRC-STRI-01-0161 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-0161 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 3e-84 52% ...

  16. NCBI nr-aa BLAST: CBRC-STRI-01-2502 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2502 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8V...IC7|VN1A2_MOUSE RecName: Full=Vomeronasal type-1 receptor A2; Short=mV1R2; AltName: Full=Pheromone receptor ...2; AltName: Full=Vomeronasal type-1 receptor A9 dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|AAI40265.1| Vomeronasa...l 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasal 1 receptor, A2 [Mus musculus] NP_035814.2 5e-70 49% ...

  17. Carboxy terminated rubber based on natural rubber grafted with acid anhydrides and its adhesion properties

    International Nuclear Information System (INIS)

    Klinpituksa, P; Kongkalai, P; Kaesaman, A

    2014-01-01

    The chemical modification of natural rubber by grafting of various polar functional molecules is an essential method, improving the versatility of rubber in applications. This research investigated the preparation of natural rubber-graft-citraconic anhydride (NR-g-CCA), natural rubber-graft-itaconic anhydride (NR-g-ICA), and natural rubber-graft-maleic anhydride (NR-g-MA), with the anhydrides grafted to natural rubber in toluene using benzoyl peroxide as an initiator. Variations of monomer content, initiator content, temperature and reaction time of the grafting copolymerization were investigated. The maximum degrees of grafting were 1.06% for NR-g-CCA, 4.66% for NR-g-ICA, and 5.03% for NR-g-MA, reached using 10 phr citraconic anhydride, 10 phr of itaconic anhydride, or 8 phr of maleic anhydride, 3 phr benzoyl peroxide, at 85, 80 and 80°C for 2, 2 and 3 hrs, respectively. Solvent-based wood adhesives were formulated from these copolymers with various contents of wood resin in the range 10-40 phr. The maximal 289 N/in cleavage peel and 245.7 KPa shear strength for NR-g-MA (5.03% grafting) were obtained at 40 phr wood resin

  18. NCBI nr-aa BLAST: CBRC-MMUS-02-0385 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-02-0385 gb|AAK56078.1|AF332049_1 adrenergic receptor alpha 2B [Mus muscul...us] gb|AAA37131.1| alpha-2 adrenergic receptor gb|EDL28187.1| adrenergic receptor, alpha 2b [Mus musculus] AAK56078.1 0.0 99% ...

  19. Permanent magnetic field, direct electric field, and infrared to reduce blood glucose level and hepatic function in mus musculus with diabetic mellitus

    Science.gov (United States)

    Suhariningsih; Basuki Notobroto, Hari; Winarni, Dwi; Achmad Hussein, Saikhu; Anggono Prijo, Tri

    2017-05-01

    Blood contains several electrolytes with positive (cation) and negative (anion) ion load. Both electrolytes deliver impulse synergistically adjusting body needs. Those electrolytes give specific effect to external disturbance such as electric, magnetic, even infrared field. A study has been conducted to reduce blood glucose level and liver function, in type 2 Diabetes Mellitus patients, using Biophysics concept which uses combination therapy of permanent magnetic field, electric field, and infrared. This study used 48 healthy mice (mus musculus), male, age 3-4 weeks, with approximately 25-30 g in weight. Mice was fed with lard as high fat diet orally, before Streptozotocin (STZ) induction become diabetic mice. Therapy was conducted by putting mice in a chamber that emits the combination of permanent magnetic field, electric field, and infrared, every day for 1 hour for 28 days. There were 4 combinations of therapy/treatment, namely: (1) permanent magnetic field, direct electric field, and infrared; (2) permanent magnetic field, direct electric field, without infrared; (3) permanent magnetic field, alternating electric field, and infrared; and (4) permanent magnetic field, alternating electric field, without infrared. The results of therapy show that every combination is able to reduce blood glucose level, AST, and ALT. However, the best result is by using combination of permanent magnetic field, direct electric field, and infrared.

  20. Gene : CBRC-MMUS-06-0060 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0060 6 A Pheromone receptors V1A16_RAT 2e-54 41% ref|NP_598937.1| vomeronasa...l 1 receptor, C21 [Mus musculus] gb|AAL47882.1| vomeronasal receptor V1RC21 [Mus musculus] gb|AAI32387.1| Vomeronasa...-length enriched library, clone:C230065D10 product:similar to VOMERONASAL RECEPTOR V1RC3 [Mus musculus], ful

  1. NCBI nr-aa BLAST: CBRC-OLAT-12-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-12-0001 gb|AAK56078.1|AF332049_1 adrenergic receptor alpha 2B [Mus muscul...us] gb|AAA37131.1| alpha-2 adrenergic receptor gb|EDL28187.1| adrenergic receptor, alpha 2b [Mus musculus] AAK56078.1 1e-102 49% ...

  2. Effect of Powder Leaf Breadfruit Disposals (Arthocarpus Altilis) in Oil Mandar District and Polman Against Cholesterol and Glucose Mice (Mus Musculus)

    Science.gov (United States)

    Mu'nisa, A.; Asmawati, A.; Farida, A.; FA, Fressy; Erni

    2018-01-01

    The purpose of this study was to determine the effect of powdered leaves of breadfruit (Arthocarpus altilis) on oil is mandated origin of the Polman glucose and cholesterol levels in mice (Mus musculus). This study comprised 4 treatments and each treatment consisted of 5 replicates, ie groups of mice were fed a standard (negative control); 2 groups: group of mice fed with standard and cholesterol feed (positive control); Group 3 that mice fed with standard and Selayar oil; and group 4: group of mice fed with standard and Mandar oil that has been given powdered leaves of breadfruit. Measurement of glucose and blood cholesterol levels in mice done 3 times ie 2 weeks after the adaptation period (phase 1), 2 weeks after administration of the oil (phase 2) and 2 weeks after feeding cholesterol (stage 3). Based on the analysis of data both cholesterol and glucose levels showed that in a group of 4 decreased glucose and cholesterol levels in stage 2 but at stage 3 an increase in the group of mice given only the oil while in the group of mice given the oil and powdered leaves of breadfruit indicate glucose levels and normal cholesterol. The conclusion of this study show that the addition of powdered leaves of breadfruit into cooking oil Mandar influential in glucose levels and normalize blood cholesterol levels in mice.

  3. Effect of Pasak Bumi (Eurycoma longifolia Jack) Root In Precopulation Stage to the Fertility of Female Mouse (Mus musculus L.)

    OpenAIRE

    Marlinza, Rosa

    2012-01-01

    Pasak Bumi (Eurycoma longifolia Jack) have potency to be used to increase bodyendurance, to cure malaria drug, and to act as afrodisiak. However, the effect of pasak bumi onwomen fertility, especially at pre-copulation stage was not widely known. This research seeks toreveal the effect pasak bumi extract treated at pre-copulation phase on fertility. This experimentemploy mice (Mus Musculus L.) and was undertaken at Biology and Cemistry laboratories PMIPA, andVeterinary laboratory of Jambi Uni...

  4. PENGARUH TRITERPEN TOTAL PEGAGAN (Centella asiatica (L Urban TERHADAP FUNGSI KOGNITIF BELAJAR DAN MENGINGAT PADA MENCIT JANTAN ALBINO (Mus musculus YANG DIHAMBAT DENGAN SKOPOLAMIN

    Directory of Open Access Journals (Sweden)

    Herlina

    2010-11-01

    Full Text Available Pegagan (Centella asiatica (L Urban has been described to posses CNS effects such as improving cognitive function, learning and memory. The aim of the research was to evaluate the effects of total triterpen’s pegagan extract on cognitive functions as the learning and memory performance in male albino mice (Mus musculus inhibited by scopolamine. The research design was Complete Randomized Design (RAL – factorial on thirty six mice divided into 4 groups. One control group received only aquabidest (negative control. Three treatment groups received total triterpen 16 mg/kg BW, 32 mg/kg BW orally and piracetam 500 mg/kg BW by intra peritoneally (positive control for 21 days. Data indicating learning and memory process of all subjects were obtained from one-trial passive avoidance test. Data were analyzed by two way ANOVA and BNT (p0,05. In conclusion, total triterpen from pegagan (Centella asiatica (L Urban improved learning ability and memory of male albino mice (Mus musculus even though, it was inhibited by scopolamine.

  5. Classical Mus musculus Igκ enhancers support transcription but not high level somatic hypermutation from a V-lambda promoter in chicken DT40 cells.

    Directory of Open Access Journals (Sweden)

    Naga Rama Kothapalli

    2011-04-01

    Full Text Available Somatic hypermutation (SHM of immunoglobulin genes is initiated by activation-induced cytidine deaminase (AID in activated B cells. This process is strictly dependent on transcription. Hence, cis-acting transcriptional control elements have been proposed to target SHM to immunoglobulin loci. The Mus musculus Igκ locus is regulated by the intronic enhancer (iE/MAR and the 3' enhancer (3'E, and multiple studies using transgenic and knock-out approaches in mice and cell lines have reported somewhat contradictory results about the function of these enhancers in AID-mediated sequence diversification. Here we show that the M. musculus iE/MAR and 3'E elements are active solely as transcriptional enhancer when placed in the context of the IGL locus in Gallus gallus DT40 cells, but they are very inefficient in targeting AID-mediated mutation events to this locus. This suggests that either key components of the cis-regulatory targeting elements reside outside the murine Igκ transcriptional enhancer sequences, or that the targeting of AID activity to Ig loci occurs by largely species-specific mechanisms.

  6. [Effect of heterozygosity for insertions of homogeneously stained regions in chromosome 1 of the house mouse on synapsis in meiotic prophase].

    Science.gov (United States)

    Borodin, P M; Ladygina, T Iu; Gorlov, I P

    1989-02-01

    Electron microscope analysis of surface-spread synaptonemal complexes (SC) in oocytes and spermatocytes from double cis heterozygotes for Is(HSR; 1C5)1Icg and Is(HSR; 1E3)2Icg was carried out. Aberrant chromosomes were isolated from the feral population of Mus musculus musculus of Novosibirsk. They contain homogeneously stained regions of total length of about 30% of Chr 1 mitotic metaphase. Heteromorphic bivalents of Chr1 with different lengths of the lateral elements of SC and the loop in the intermedial position were revealed in 4.4% spermatocytes and 20% oocytes of heterozygous animals. The loop size depends on the stage of meiosis: it is maximal at late zygotene and decreases up to disappearance during pachytene.

  7. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti Wahyuningsih

    2016-03-01

    Full Text Available Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1 and adding polysaccharide krestin before-after exposure (P3 had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2 had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016. Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1, 12-17.

  8. Personal health records in the Netherlands: potential user preferences quantified by a discrete choice experiment.

    Science.gov (United States)

    Determann, Domino; Lambooij, Mattijs S; Gyrd-Hansen, Dorte; de Bekker-Grob, Esther W; Steyerberg, Ewout W; Heldoorn, Marcel; Pedersen, Line Bjørnskov; de Wit, G Ardine

    2017-05-01

    To identify groups of potential users based on their preferences for characteristics of personal health records (PHRs) and to estimate potential PHR uptake. We performed a discrete choice experiment, which consisted of 12 choice scenarios, each comprising 2 hypothetical PHR alternatives and an opt-out. The alternatives differed based on 5 characteristics. The survey was administered to Internet panel members of the Dutch Federation of Patients and Consumer Organizations. We used latent class models to analyze the data. A total of 1,443 potential PHR users completed the discrete choice experiment. We identified 3 latent classes: "refusers" (class probability 43%), "eager adopters" (37%), and "reluctant adopters" (20%). The predicted uptake for the reluctant adopters ranged from 4% in the case of a PHR with the worst attribute levels to 68% in the best case. Those with 1 or more chronic diseases were significantly more likely to belong to the eager adopter class. The data storage provider was the most decisive aspect for the eager and reluctant adopters, while cost was most decisive for the refusers. Across all classes, health care providers and independent organizations were the most preferred data storage providers. We identified 3 groups, of which 1 group (more than one-third of potential PHR users) indicated great interest in a PHR irrespective of PHR characteristics. Policymakers who aim to expand the use of PHRs will be most successful when health care providers and health facilities or independent organizations store PHR data while refraining from including market parties. © The Author 2016. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  9. FRAKSI ETANOL 96% BIJI KORO BENGUK (Mucuna pruriens L. SEBAGAI PENINGKAT KUALITAS SPERMATOZOA MENCIT (Mus musculus

    Directory of Open Access Journals (Sweden)

    Sri Winarni

    2012-11-01

    Full Text Available Background: The examination of sperm quality is the main priority for infertility diagnosis. Based on previous study with mice, active ingredient of Mucuna pruriens L. or koro benguk (Papilionaceae, the L-dopa, may affect the quality of spermatozoa.Objective: Research was to study the effect of 96% ethanol fraction Mucuna pruriens seed on spermatozoaquality of mice exposed to 2-Methoxy ethanol. L-dopa in 96% ethanol fraction of M. pruriens seed was 14.7%.Methode: This was an experimental study using complete randomized design. Subjects were BALB/C mice (Mus musculus. Five groups served as control, 3 groups received subcutaneos injection of 2-ME as muchas 100 mg/kg.bw/day for 12 days, followed with 96% ethanol fraction Mucuna pruriens seed starting from14 mg/kg.bw/day, 28 mg/kg.bw/day, and 56 mg/kg.bw/day for 51 days.Result: The 96% ethanol fraction of Mucuna pruriens seeds are significant increase motility (p<0,01 andthe percentage of normal spermatozoa morphology (p= 0,042.Conclusion: 96% ethanol fraction of Mucuna pruriens seeds are able to increase motility and the percentage of normal spermatozoa morphology in mice exposed to 2-ME. Keywords: Mucuna pruriens L., L-dopa, mouse spermatozoa

  10. Vulcanization of Rubber

    Indian Academy of Sciences (India)

    Temperature 140 -180°C. Sulfur. 2-3 parts per. 100 parts of rubber (phr). Accelerator 0.5-1.0 phr. ZnO ... out an experiment, he spilt a mixture of rubber and sulfur with other ingredients on a hot .... both carbon-sulfur and sulfur-nitrogen bonds -.

  11. Patient Health Record Systems Scope and Functionalities: Literature Review and Future Directions.

    Science.gov (United States)

    Bouayad, Lina; Ialynytchev, Anna; Padmanabhan, Balaji

    2017-11-15

    A new generation of user-centric information systems is emerging in health care as patient health record (PHR) systems. These systems create a platform supporting the new vision of health services that empowers patients and enables patient-provider communication, with the goal of improving health outcomes and reducing costs. This evolution has generated new sets of data and capabilities, providing opportunities and challenges at the user, system, and industry levels. The objective of our study was to assess PHR data types and functionalities through a review of the literature to inform the health care informatics community, and to provide recommendations for PHR design, research, and practice. We conducted a review of the literature to assess PHR data types and functionalities. We searched PubMed, Embase, and MEDLINE databases from 1966 to 2015 for studies of PHRs, resulting in 1822 articles, from which we selected a total of 106 articles for a detailed review of PHR data content. We present several key findings related to the scope and functionalities in PHR systems. We also present a functional taxonomy and chronological analysis of PHR data types and functionalities, to improve understanding and provide insights for future directions. Functional taxonomy analysis of the extracted data revealed the presence of new PHR data sources such as tracking devices and data types such as time-series data. Chronological data analysis showed an evolution of PHR system functionalities over time, from simple data access to data modification and, more recently, automated assessment, prediction, and recommendation. Efforts are needed to improve (1) PHR data quality through patient-centered user interface design and standardized patient-generated data guidelines, (2) data integrity through consolidation of various types and sources, (3) PHR functionality through application of new data analytics methods, and (4) metrics to evaluate clinical outcomes associated with automated PHR

  12. Embryo quality of mice (“Mus musculus” fed royal jelly Qualidade embrionária de camundongos ("Mus musculus" suplementados com geléia real

    Directory of Open Access Journals (Sweden)

    Bruno Edésio dos Santos Melo

    2009-03-01

    Full Text Available The study was carried out to verify the effect of feeding royal jelly associated to follicle growth induction on number and quality of mice (Mus musculus embryos. Sixty Swiss females ageing from eight to ten weeks were distributed into three treatments: the first one, composed by animals fed 0.2 mL of physiological solution intraperitoneal (control group, n=20; the second and third ones, composed by females fed 0.5 and 1.0 mg of royal jelly diluted in 0.2 mL of physiological solution (n=20, respectively. Royal jelly was administered during 15 days, followed by the follicular growth induction process. Embryos were collected 68 hours after mating, by uterine flushing. No treatment effects on the number of females that answered to superovulatory process, the number of total recovery structures, the number of viable and non viable structures and the morphological quality of viable embryos (P>0.05 were observed. Therefore, royal jelly doses used were not efficient to increase the number of embryos and to improve the quality recovery from superovulated mice.Objetivou-se verificar a influência da geléia real, associada ao tratamento de indução de crescimento folicular, no número e na qualidade de embriões de camundongos (Mus musculus. Foram utilizadas 60 fêmeas da linhagem Suíço albino com idade entre oito e dez semanas, distribuídas em três tratamentos: o primeiro, composto por animais que receberam 0,2 mL de solução fisiológica, via intraperitonial (grupo controle, n=20; o segundo e terceiro, compostos de fêmeas que receberam 0,5 e 1 mg de geléia real diluídos em 0,2 mL de solução fisiológica via intraperitonial (n=20, respectivamente. Foi administrada geléia real por um período de 15 dias, segundo o processo de indução do crescimento folicular. As coletas dos embriões ocorreram 68 horas após a cobertura, utilizando-se o método da lavagem uterina. Não houve diferença no número de fêmeas que responderam ao tratamento

  13. NCBI nr-aa BLAST: CBRC-OLAT-26-0164 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-26-0164 ref|NP_031715.1| chondroadherin [Mus musculus] sp|O55226|CHAD_MOUSE Chondroad...herin precursor (Cartilage leucine-rich protein) gb|AAC39963.1| chondroadherin [Mus musculus] gb|AAH12672.1| Chondroad...herin [Mus musculus] emb|CAI25589.1| chondroadherin [Mus musculus] dbj|BAE34451.1| unn...amed protein product [Mus musculus] gb|EDL15934.1| chondroadherin [Mus musculus] NP_031715.1 5e-85 57% ...

  14. NCBI nr-aa BLAST: CBRC-TNIG-03-0019 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-03-0019 ref|NP_059087.2| solute carrier family 5 (inositol transporters),... member 3 [Mus musculus] gb|EDL03793.1| solute carrier family 5 (inositol transporters), member 3 [Mus musculus] NP_059087.2 1e-113 51% ...

  15. Permanent magnetic field, direct electric field, and infrared to reduce blood glucose level and hepatic function in mus musculus with diabetic mellitus

    International Nuclear Information System (INIS)

    Suhariningsih; Prijo, Tri Anggono; Notobroto, Hari Basuki; Winarni, Dwi; Hussein, Saikhu Achmad

    2017-01-01

    Blood contains several electrolytes with positive (cation) and negative (anion) ion load. Both electrolytes deliver impulse synergistically adjusting body needs. Those electrolytes give specific effect to external disturbance such as electric, magnetic, even infrared field. A study has been conducted to reduce blood glucose level and liver function, in type 2 Diabetes Mellitus patients, using Biophysics concept which uses combination therapy of permanent magnetic field, electric field, and infrared. This study used 48 healthy mice ( mus musculus ), male, age 3-4 weeks, with approximately 25-30 g in weight. Mice was fed with lard as high fat diet orally, before Streptozotocin (STZ) induction become diabetic mice. Therapy was conducted by putting mice in a chamber that emits the combination of permanent magnetic field, electric field, and infrared, every day for 1 hour for 28 days. There were 4 combinations of therapy/treatment, namely: (1) permanent magnetic field, direct electric field, and infrared; (2) permanent magnetic field, direct electric field, without infrared; (3) permanent magnetic field, alternating electric field, and infrared; and (4) permanent magnetic field, alternating electric field, without infrared. The results of therapy show that every combination is able to reduce blood glucose level, AST, and ALT. However, the best result is by using combination of permanent magnetic field, direct electric field, and infrared. (paper)

  16. The Southeastern Asian house mouse (Mus musculus castaneus Linn.) as a new passenger host for Cryptococcus neoformans var. grubii molecular type VNI.

    Science.gov (United States)

    Singh, Karuna; Rani, Jyoti; Neelabh; Rai, Govind Kumar; Singh, Major

    2017-11-01

    We describe Mus musculus castaneus as a new mammalian host for Cryptococcus neoformans var. grubii (VNI). Eighteen apparently healthy adults and pups of the rodent were collected from human dwellings in Varanasi, a city of India. Both clinical and behavioral examinations of the rodents did not reveal any sign of the disease. Among visceral organs, histological examination of only liver exhibited the presence of single celled, encapsulated, Southgate's mucicarmine positive fungal structures consistent with C. neoformans. Nevertheless, culture of tissue homogenates of brain, lungs, liver, and kidneys yielded white colonies on Sabouraud's dextrose agar and brown mucoid colonies of C. neoformans on Staib's and Tobacco agar media. The pathogen was isolated from habitat soil as well as fresh faeces of the animals. All isolates were urease positive, nitrate and canavanine-glycine bromothymol blue negative, exhibited phenoloxidase activity and grew at 37°C. The isolates were identified as C. neoformans var. grubii with ITS primers and unique marker (GACA)4. The pathogen when inoculated in immunosuppressed mice showed low pathogenicity. To our knowledge, we for the first time report case cluster of Mus musculus castaneus as new passenger host for C. neoformans var. grubii (VNI). © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  17. Starch derivative-based superabsorbent with integration of water-retaining and controlled-release fertilizers.

    Science.gov (United States)

    Zhong, Kang; Lin, Zuan-Tao; Zheng, Xi-Liang; Jiang, Gang-Biao; Fang, Yu-Sheng; Mao, Xiao-Yun; Liao, Zong-Wen

    2013-02-15

    Phosphate rock (PHR), a traditional fertilizer, is abundant, but is hard to be utilized by plants. To improve the utilization of PHR, and to integrate water-retaining and controlled-release fertilizers, an agricultural superabsorbent polymer based on sulfonated corn starch/poly (acrylic acid) embedding phosphate rock (SCS/PAA/PHR) was prepared. PHR can be suspended and well-dispersed in SCS/PAA by sulfonated corn starch (SCS). PHR and KOH were mixed in acrylic acid solution to provide phosphorus (P) and potassium (K) nutrients, respectively. Impacts on water absorption capacity of the superabsorbent were investigated. The maximum swelling capacity in distilled water or 0.9 wt.% (weight percent) NaCl solution reached 498 g g(-1) and 65 g g(-1) (water/prepared dry superabsorbent) respectively. Moreover, release behaviours of P and K in SCS/PAA/PHR were also investigated. The results showed that SCS/PAA/PHR possessed excellent sustained-release property of plant nutrient, and the SCS/PAA could improve the P release greatly. Besides, the XPS analysis was employed to study the relationship between PHR and superabsorbent polymer. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. The Added Value of Log File Analyses of the Use of a Personal Health Record for Patients With Type 2 Diabetes Mellitus: Preliminary Results.

    Science.gov (United States)

    Sieverink, Floor; Kelders, Saskia M; Braakman-Jansen, Louise M A; van Gemert-Pijnen, Julia E W C

    2014-03-01

    The electronic personal health record (PHR) is a promising technology for improving the quality of chronic disease management. Until now, evaluations of such systems have provided only little insight into why a particular outcome occurred. The aim of this study is to gain insight into the navigation process (what functionalities are used, and in what sequence) of e-Vita, a PHR for patients with type 2 diabetes mellitus (T2DM), to increase the efficiency of the system and improve the long-term adherence. Log data of the first visits in the first 6 weeks after the release of a renewed version of e-Vita were analyzed to identify the usage patterns that emerge when users explore a new application. After receiving the invitation, 28% of all registered users visited e-Vita. In total, 70 unique usage patterns could be identified. When users visited the education service first, 93% of all users ended their session. Most users visited either 1 or 5 or more services during their first session, but the distribution of the routes was diffuse. In conclusion, log file analyses can provide valuable prompts for improving the system design of a PHR. In this way, the match between the system and its users and the long-term adherence has the potential to increase. © 2014 Diabetes Technology Society.

  19. AcEST: DK953370 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PS2_MOUSE RNA-binding protein with multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length = 206 Scor...NA-binding protein with multiple splicing OS=Mus musculus GN=Rbpms PE=2 SV=1 Leng...FDPEIPQTLRLEFAKANTK 104 >sp|Q9YGP5|RBPMS_XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms

  20. AcEST: DK950959 [AcEST

    Lifescience Database Archive (English)

    Full Text Available XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms PE=2 SV=1 Length = 196 Score = 4...iple splicing OS=Mus musculus GN=Rbpms PE=2 SV=1 Length = 197 Score = 47.8 bits (112), Expect = 6e-05 Identi...nding protein with multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length = 206 Score = 46.2 bits (1

  1. Efecto de las nanopartículas industriales TiO 2 , SiO 2 y ZnO sobre la viabilidad celular y expresión génica en médula ósea roja de mus musculus

    Directory of Open Access Journals (Sweden)

    Jacquelyne Zarria-Romero

    Full Text Available RESUMEN Objetivos Evaluar el efecto de las nanopartículas de ZnO, TiO2 y SiO2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito - macrófago (GM-CSF en Mus musculus. Materiales y métodos Se extrajo médula ósea roja de cinco roedores (Balb/c para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas de TiO2, 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados Las nanopartículas de ZnO y SiO2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente, con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO2 (91%. Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones Las nanopartículas de ZnO, SiO2 y TiO2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón.

  2. Laju peningkatan konsentrasi timbal Pb dalam darah hewan uji mencit Mus musculus di bengkel otomotif

    Directory of Open Access Journals (Sweden)

    Mohammad Razif Sukatma

    2012-02-01

    Full Text Available This research was conducted in automotive workshop of VEDC Malang and the samples were taken at the automotive machineand automotive electric workshops. The parameters which examined were the Pb concentration in the mouse blood (Mus musculus in2 and 3 months years old. The measurement was run for 5 times every 10 days, and the result was mean value of the increasing rate ofPb concentration in the mouse blood. The aim of this research was to determine the increasing rate of Pb concentration in the mouseblood which has the relation with different working area, different age, and also duration of exposure. Based on the data of laboratorytest, research continued by T-test analysis, Simple regression test, and correlation, and also General Linear Model. By doing statisticanalysis with the different working area, duration of explanation, and different age.Result of this research showed that there were the differences of rapid increasing of Pb concentration in both workshop. Generallythe age which influence so much for that (in the mouse body and have the relation with the body stamina. If the mouse body absorbamount of Pb constantly, so the Pb concentration in it will increase more and more and it cause the decreasing of the body height.

  3. The Sycp1 loci of the mouse genome: successive retropositions of a meiotic gene during the recent evolution of the genus.

    Science.gov (United States)

    Sage, J; Yuan, L; Martin, L; Mattei, M G; Guénet, J L; Liu, J G; Hoög, C; Rassoulzadegan, M; Cuzin, F

    1997-08-15

    The murine Sycp1 gene is expressed at the early stages of meiosis. We show that it is composed of a number of small exons and localized on mouse chromosome 3. In the laboratory strains, two retrogenes were also identified. The first one (Sycp1-ps1), on chromosome 7, has accumulated point mutations and deletions and is not transcribed. A second retrogene (Sycp1-ps2), on chromosome 8, is inserted within the continuity of a moderately repeated element, in an intron of another gene (Cad11). The two retroposition events can be dated to distinct periods in the evolution of the Muridae. Sycp1-ps2 has kept features indicative of a relatively recent origin, namely a nearly intact coding region, a poly(A) tail, and 14-bp terminal repeats. Its recent origin was confirmed by the fact that it is found in all the laboratory strains of mice, but neither in a recent isolate from Mus musculus domesticus wild stocks nor in the closely related subspecies M. musculus musculus, M. m. molossinus, M. m. castaneus, and M. m. bactrianus. Appearance of the more ancient Sycp1-ps1 retrogene is concomitant with the radiation of the genus. It is present in various Mus species (M. spretus, M. spicilegus, M. macedonicus, and M. cookii), but neither in the rat nor in the more closely related Pyromis genus. Transposition of retrotranscripts during meiosis and their hereditary establishment thus appear to occur relatively frequently. They may, therefore, play a significant role in the evolutionary process.

  4. Caregiver preferences regarding Personal Health Records in the management of ADHD

    Science.gov (United States)

    Ronis, Sarah D; Baldwin, Constance D; McIntosh, Scott; McConnochie, Kenneth; Szilagyi, Peter G; Dolan, James

    2015-01-01

    Objective PHRs may address the needs of children with ADHD. Among parents, we assessed acceptance, barriers, and intentions regarding use of PHR for their children with ADHD. Methods Survey of parents from three practices in Rochester NY. Stepwise logistic regression analysis explored factors predicting respondents’ intentions for future use of PHR, accounting for care coordination needs, caregiver education, SES and satisfaction with providers. Results Of 184 respondents, 23% had used the PHR for their child, 82% intended future use. No difference was observed between users and non-users regarding gender, age, race, or education. Users were more likely than non-users to reside in the suburbs (p=0.03). Caregivers were more likely to plan future use of the PHR if they felt engaged as partners in their child’s care (AOR 2.3, 95%CI 1.2, 4.5). Conclusions Parents are enthusiastic about PHRs. Future work should focus on engaging them as members of the healthcare team. PMID:25567294

  5. Dicty_cDB: Contig-U12108-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _tissue_mixed_st... 48 0.37 1 ( EK415671 ) 1095515461096 Global-Ocean-Sampli...( BC087733 |pid:none) Mus musculus transcription termina... 95 2e-18 (Q7ZU90) RecName: Full=Chromodomain-heli...095458006916 Global-Ocean-Sampling_GS-31-01-01-1... 36 2.6 2 ( DR395822 ) USDA-FP_155757 Adult Alate Aphis g...06f CUGI Rice BAC Library Oryza sativa J... 44 5.7 1 ( EJ951574 ) 1093018948721 Global-Ocean-Sampling_GS-30-...02-01-1... 44 5.7 1 ( EJ038559 ) 1095454082808 Global-Ocean-Sampling_GS-26-01-01-

  6. AcEST: DK950692 [AcEST

    Lifescience Database Archive (English)

    Full Text Available |Q8VC52|RBPS2_MOUSE RNA-binding protein with multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length ...ultiple splicing OS=Mus musculus GN=Rbpms PE=2 SV=1 Length = 197 Score = 77.0 bits (188), Expect = 8e-14 Ide...S_XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms PE=2 SV=1 Length = 196 Score =

  7. NCBI nr-aa BLAST: CBRC-OCUN-01-0428 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0428 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9EQ52|VN1A3_MOUSE Vomeronas...al type-1 receptor A3 (Vomeronasal type-1 receptor A6) gb|AAG42076.1|AF291482_1 vomeronasa...l receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 [Mus musculus] gb|AAI41612.1| Vomeronasa...l 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasal 1 receptor, A3 [synthetic construct] NP_444448.1 2e-82 51% ...

  8. AcEST: DK955384 [AcEST

    Lifescience Database Archive (English)

    Full Text Available >sp|Q9WVB0|RBPMS_MOUSE RNA-binding protein with multiple splicing OS=Mus musculus GN=Rbpms...OUSE RNA-binding protein with multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length = 206 Score = 5...XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms PE=2 SV=1 Length = 196 Score = 5

  9. Determination of muscle microcirculation of the limbs of healthy persons and patients with scleroderma by means of 133Xe clearance. 2

    International Nuclear Information System (INIS)

    Poenitzsch, I.; Wiemers, U.; Haustein, U.F.; Schneider, G.

    1984-01-01

    By means of 133 Xe muscle clearance the blood flow of the musculus tibialis anterior and the musculus opponens pollicis was determined during nonischemic work and after 3 minutes ischemia in patients with progressive scleroderma and additionally of the ischemic musculus opponens pollicis following contrast baths. In relation to 53 patients with normal vessels the reactive hyperemia of the musculus tibialis anterior after ischemia and of the musculus opponens pollicis after heat, cold as well as arterial flow decreasing was significantly decreased in patients with scleroderma and was as a disturbance of the microcirculation realized. For scientific problems in progressive scleroderma the 133 Xe muscle clearance is suitable as to the musculus opponens pollicis. (author)

  10. Musculus soleus of rats at physical activity and L-carnitine and creatine phosphate effect

    Directory of Open Access Journals (Sweden)

    Irina A. Khutorskaya

    2017-09-01

    Full Text Available Introduction: The study of the effect of metabolic drugs on the histochemical characteristics of soleus muscle is relevant for solving the problem of providing the training process in Russia with non-doping drugs for safe correction of the consequences of intense physical activity in athletes. Materials and Methods: Dynamic physical activity in rats (n = 24 was simulated by swimming “to the limit” with weighting of 10 % of body weight (20 days, 1 time per day. The experimental animals were divided into four groups (6 animals each: № 1 – control, № 2 – swimming + isotonic NaCl solution, № 3 and № 4 – swimming + L-carnitine or creatine phosphate 100.0 mg/kg daily intraperitoneally. The object of the study was musculus soleus. Differentiation of muscle fibers was carried out by the intensity of histochemical activity of succinate dehydrogenase (SDG and alkaline stable adenosine triphosphate (ATP of myosin. The percentage of muscle fibers was evaluated and their diameter was defined by the direct morphometry. The obtained data were treated statistically by Student’s T-test. Results: Swimming of the animals “to the limit” do not affect the ratio of fibers with different phenotypes in the soleus muscle. This indicator is genetically determined and was not modified by L-carnitine and creatine phosphate. Dynamic physical activity promotes the development of hypertrophy of muscle fibers of various types. The investigated medicaments of the metabolic type either do not influence on the formation of exerciseinduced hypertrophy (predominantly creatine phosphate or reduce the intensity of the hypertrophic process (predominantly L-carnitine under dynamic physical activity. Discussion and Conclusions: The obtained data indicate L-carnitine and creatine phosphate do not have an anabolic effect. Taking into account the relevant data on ability of L-carnitine and creatine phosphate to effectively correct a negative effects of intensive

  11. PENGARUH DOSIS DAN LAMA PERLAKUAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn. TERHADAP STRUKTUR HISTOLOGI GINJAL MENCIT (Mus musculus L.

    Directory of Open Access Journals (Sweden)

    Nur Assiam

    2014-09-01

    Full Text Available This research aims to determine the effect of red calliandra (Calliandra calothyrsusMeissn. leaf extrac on the histological structure of the kidney of mice (Mus musculus L..Treatment was administered orally with varying doses. This research used a CompletelyRandomized Design in factorial pattern of two factors, doses (0 or control, 2, 4, and 6 mg/kgBW and length of treatment (7, 14, and 21 days, so there are 12 combination groups with 3replications of each. Organ was collected on days 8, 15, and 22 to observe histologicalstructure of the kidney. Renal histological observation of edema, Bowman’s spaceconstriction, and protein deposition, showed no correlation between both factors, but a veryreal correlation occurs in the damage of fatty degeneration, hemorrhage, and nucleuspyknotic. Histological observation of glomerular congestion and infiltration of inflammatorycells did not show any correlation between dose and duration of treatment.Keywords: red calliandra, histopathology of kidney, male mice

  12. ORF Alignment: NT_033777 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available NT_033777 gi|28571958 >1bor0 1 53 282 342 4e-04 ... ref|NP_083045.3| synoviolin 1 [Mu...s musculus] gb|AAH42199.1| Synoviolin 1 [Mus ... musculus] gb|AAH80722.1| Synoviolin 1 [Mus musculus]... ... gb|AAH57917.1| Synoviolin 1 [Mus musculus] ... Length = 61 ... Query: 280 EELRQSDNICIICREDMV

  13. Hubungan Hiperglikemia dengan Prothrombin Time pada Mencit (Mus musculus yang Diinduksi Aloksan

    Directory of Open Access Journals (Sweden)

    Muhammad Ibnu Malik

    2015-01-01

    . Endothelial disfungtion can be detected by activated partial thromboplastin time (APTT and prothrombin time (PT. The objective of this studi was to determine the correlation between hyperglycemia and prothrombin time in mice (Mus musculus induced with aloxan. The design of this research was a post test only control group design conducted in October 2013 until February 2014 in Central Laboratory RS Dr. M. Djamil Padang. The subject were white mice (Mus musculus who have met the inclusion and exclusion criteria. The subject were divided as hyperglycemia group (induced with aloxan and control group. After seven days of adaptation, the aloxan was injected and measurenment of blood glucose and body weight had been done, one time in every four days. Then in day 30th the termination of mice had been done to meassure the prothrombin time. The result showed the prothrombin time between group was shortened with the average prothrombin time of the control group was 7,96 second and the hiperglicemia group was 8,12 second. The result showing no correlation between hyperglycemia and prothrombin time with the degree of signification is (p 0,7 (p > 0,05.Keywords: hyperglycemia, diabetes mellitus, prothrombin time,

  14. NCBI nr-aa BLAST: CBRC-BTAU-01-2652 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available s musculus] gb|AAH17129.1| Solute carrier family 16 (monocarboxylic acid transporters), member 6 [Mus muscul...us] emb|CAM25213.1| solute carrier family 16 (monocarboxylic acid transporters), member 6 [Mus musculus] gb|...EDL34359.1| solute carrier family 16 (monocarboxylic acid transporters), member 6..., isoform CRA_a [Mus musculus] gb|EDL34361.1| solute carrier family 16 (monocarboxylic acid transporters), member 6, isoform CRA_a [Mus musculus] NP_598799.1 1e-110 76% ...

  15. NCBI nr-aa BLAST: CBRC-PCAP-01-1368 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-1368 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  16. NCBI nr-aa BLAST: CBRC-PHAM-01-1594 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1594 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  17. NCBI nr-aa BLAST: CBRC-MLUC-01-1112 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-1112 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  18. NCBI nr-aa BLAST: CBRC-OPRI-01-0982 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0982 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  19. NCBI nr-aa BLAST: CBRC-VPAC-01-1554 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1554 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  20. NCBI nr-aa BLAST: CBRC-MMUR-01-1494 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-1494 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  1. NCBI nr-aa BLAST: CBRC-TTRU-01-0117 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0117 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  2. NCBI nr-aa BLAST: CBRC-GGOR-01-1297 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGOR-01-1297 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE RecName: Full=Cannabinoid receptor 1; Short=CB1; Short=CB-R; AltName: Full=Brain-type cann...abinoid receptor gb|AAD34624.1|AF153345_1 CB1 cannabinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannab...inoid receptor [Mus musculus] gb|AAA91176.1| neuronal cannabinoid receptor [Mus m...usculus] emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal cannabinoid recepto

  3. Arabidopsis MYB-Related HHO2 Exerts a Regulatory Influence on a Subset of Root Traits and Genes Governing Phosphate Homeostasis.

    Science.gov (United States)

    Nagarajan, Vinay K; Satheesh, Viswanathan; Poling, Michael D; Raghothama, Kashchandra G; Jain, Ajay

    2016-06-01

    Phosphate (Pi), an essential macronutrient required for growth and development of plants, is often limiting in soils. Pi deficiency modulates the expression of Pi starvation-responsive (PSR) genes including transcription factors (TFs). Here, we elucidated the role of the MYB-related TF HYPERSENSITIVITY TO LOW PHOSPHATE-ELICITED PRIMARY ROOT SHORTENING1 HOMOLOG2 (HHO2, At1g68670) in regulating Pi acquisition and signaling in Arabidopsis thaliana HHO2 was specifically and significantly induced in different tissues in response to Pi deprivation. Transgenic seedlings expressing 35S::GFP::HHO2 confirmed the localization of HHO2 to the nucleus. Knockout mutants of HHO2 showed significant reduction in number and length of first- and higher-order lateral roots and Pi content of different tissues compared with the wild-type irrespective of the Pi regime. In contrast, HHO2-overexpressing lines exhibited augmented lateral root development, enhanced Pi uptake rate and higher Pi content in leaf compared with the wild-type. Expression levels of PSR genes involved in Pi sensing and signaling in mutants and overexpressors were differentially regulated as compared with the wild-type. Attenuation in the expression of HHO2 in the phr1 mutant suggested a likely influence of PHR1 in HHO2-mediated regulation of a subset of traits governing Pi homeostasis. © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  4. NCBI nr-aa BLAST: CBRC-CINT-01-0186 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CINT-01-0186 ref|NP_083045.3| synovial apoptosis inhibitor 1, synoviolin [Mus ...musculus] sp|Q9DBY1|SYVN1_MOUSE E3 ubiquitin-protein ligase synoviolin precursor (Synovial apoptosis inhibit...or 1) gb|AAH42199.1| Synovial apoptosis inhibitor 1, synoviolin [Mus musculus] gb|AAH57917.1| Synovial apoptosis inhibitor 1, synovio...lin [Mus musculus] gb|AAH80722.1| Synovial apoptosis inhibitor 1, synoviolin... [Mus musculus] gb|EDL33195.1| synovial apoptosis inhibitor 1, synoviolin, isoform CRA_b [Mus musculus] NP_083045.3 1e-167 51% ...

  5. NCBI nr-aa BLAST: CBRC-CPOR-01-1147 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-1147 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  6. NCBI nr-aa BLAST: CBRC-CPOR-01-0933 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-0933 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  7. NCBI nr-aa BLAST: CBRC-MMUS-06-0130 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0130 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  8. NCBI nr-aa BLAST: CBRC-PABE-04-0003 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-04-0003 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  9. NCBI nr-aa BLAST: CBRC-OCUN-01-1001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-1001 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  10. NCBI nr-aa BLAST: CBRC-MMUS-06-0137 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0137 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  11. NCBI nr-aa BLAST: CBRC-RNOR-04-0241 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0241 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  12. NCBI nr-aa BLAST: CBRC-CPOR-01-1167 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-1167 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  13. NCBI nr-aa BLAST: CBRC-MMUS-06-0138 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0138 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  14. NCBI nr-aa BLAST: CBRC-MMUS-06-0124 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0124 ref|NP_035814.2| vomeronasal 1 receptor, A2 [Mus musculus] sp|Q8VIC7|VN1A2_MOUSE Vomeronas...al type-1 receptor A2 (Pheromone receptor 2) (Vomeronasal type-1 receptor A9) (mV1R...2) dbj|BAB79214.1| vomeronasal receptor 1 A9 [Mus musculus] gb|AAI41637.1| Vomeronasal 1 receptor, A2 [synth...etic construct] gb|AAI40265.1| Vomeronasal 1 receptor, A2 [synthetic construct] gb|EDK99273.1| vomeronasa

  15. NCBI nr-aa BLAST: CBRC-TGUT-08-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-08-0001 ref|NP_067250.2| potassium voltage-gated channel, shaker-related ...assium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] emb|CAM23761.1| potassium voltage-gated channel, shak...4.1| potassium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] NP_067250.2 0.0 79% ...

  16. NCBI nr-aa BLAST: CBRC-ACAR-01-0365 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0365 ref|NP_067250.2| potassium voltage-gated channel, shaker-related ...assium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] emb|CAM23761.1| potassium voltage-gated channel, shak...4.1| potassium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] NP_067250.2 0.0 79% ...

  17. NCBI nr-aa BLAST: CBRC-MLUC-01-0737 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0737 ref|NP_067250.2| potassium voltage-gated channel, shaker-related ...assium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] emb|CAM23761.1| potassium voltage-gated channel, shak...4.1| potassium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] NP_067250.2 0.0 87% ...

  18. NCBI nr-aa BLAST: CBRC-ETEL-01-1362 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-1362 ref|NP_067250.2| potassium voltage-gated channel, shaker-related ...assium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] emb|CAM23761.1| potassium voltage-gated channel, shak...4.1| potassium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] NP_067250.2 0.0 93% ...

  19. NCBI nr-aa BLAST: CBRC-OANA-01-2250 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2250 ref|NP_067250.2| potassium voltage-gated channel, shaker-related ...assium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] emb|CAM23761.1| potassium voltage-gated channel, shak...4.1| potassium voltage-gated channel, shaker-related subfamily, member 4 [Mus musculus] NP_067250.2 0.0 92% ...

  20. Dicty_cDB: Contig-U11295-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 382126 |pid:none) Kluyveromyces lactis strain NRRL... 120 1e-25 BC122690_1( BC122690 |pid:none) Bos taurus transcription term...ns RNA polymerase II ter... 119 2e-25 (Q9UNY4) RecName: Full=Transcription termination factor 2; ... 119 ...345( CU633901 |pid:none) Podospora anserina genomic DNA c... 116 2e-24 (Q5NC05) RecName: Full=Transcription term...|pid:none) Mus musculus transcription termina... 115 2e-24 AL596125_1( AL596125 |pid:none) Mouse DNA sequenc...se (hZF... 115 4e-24 ( P34739 ) RecName: Full=Transcription termination factor 2;

  1. Parasitic and fungal infections in synanthropic rodents in an area of urban expansion, Aracaju, Sergipe State, Brazil - doi: 10.4025/actascibiolsci.v36i1.19760

    Directory of Open Access Journals (Sweden)

    Adriana Oliveira Guimarães

    2013-09-01

    Full Text Available This study analysed the prevalence of parasitic and fungal infections in rodents in an area of urban expansion, Aracaju, Brazil. Traps were placed in the area from December 2011 to January 2013. Blood samples, faeces and hair were collected from the animals. We collected a total of 47 rodents; 44 were Rattus rattus, and 3 were Mus musculus. Parasitological evaluation revealed the cestode Hymenolepis diminuta infection in both rodent species. The nematodes Aspiculuris tetraptera and Syphacia obvelata were found in M. musculus, and the commensal Entamoeba coli was found in R. rattus. We observed that 69.2% of the R. rattus and 33.3% of the M. musculus were infected with the haemoparasite Babesia sp. The differential leukocyte count revealed normal (72.3%, neutrophilic (15.9% and lymphocytic (11.4% profiles. The evaluation showed the following species of fungi in the rodents: Aspergillus sp. (77.1%, Penicillium sp. (28.6%, Cladosporium sp. (14.3%, Mucor sp. (14.3%, Curvularia sp. (8.6%, Acremonium sp. (8.6%, Chrysosporium sp. (2.9%, Syncephalostrum sp. (2.9%, Alternaria sp. (2.9%, Trichophyton sp. (2.9% and Scopulariopsis sp. (2.9%. The parasites and fungi found in rodents are potentially zoonotic, and the presence of these household animals demonstrates their potential role as reservoirs and disseminators of fungal and parasitic infections.

  2. Transgressive segregation in a behavioural trait? Explorative strategies in two house mouse subspecies and their hybrids

    Czech Academy of Sciences Publication Activity Database

    Hiadlovská, Zuzana; Vošlajerová Bímová, Barbora; Mikula, Ondřej; Piálek, Jaroslav; Macholán, Miloš

    2013-01-01

    Roč. 108, č. 1 (2013), s. 225-235 ISSN 0024-4066 R&D Projects: GA ČR GA206/08/0640; GA ČR GAP506/11/1792; GA AV ČR KJB600930701 Institutional support: RVO:68081766 ; RVO:67985904 Keywords : dispersion * exploration * hybridization * Mus musculus domesticus * Mus musculus musculus * open field test Subject RIV: EG - Zoology; EB - Genetics ; Molecular Biology (UZFG-Y) Impact factor: 2.535, year: 2013

  3. Preparation of irradiated natural rubber latex-styrene copolymer for electrical gloves

    International Nuclear Information System (INIS)

    Made Sumarti Kardha

    2010-01-01

    Research on irradiated natural rubber latex-styrene copolymer to prepare electrical glove have been done. Vulcanization of natural rubber latex (NRL) was done by mixing 2 phr (per hundred of rubber) of normal butyl acrylate (n-BA) emulsion then irradiated with ã-ray "6"0Co at the dose of 30 kGy producing irradiated natural rubber latex (INRL). Natural rubber latex-styrene copolymers (SC) were prepared by mixing NRL and styrene monomer at styrene concentrations of 50 phr, 75 phr, 100 phr, 500 phr and irradiated at the doses of 15 kGy, 30 kGy and 45 kGy, then characterized their latex and film properties to obtain optimum SC of SC50. This optimum SC, SC50 then mixed with IRNL at the weight ratio of 0/100, 30/70, 50/50, 70/30 and 100/0, to produce irradiated natural rubber latex-styrene copolymer. The properties of copolymer rubber films made by dipping process i.e., % conversion, total solid content, latex viscosity, tensile strength, modulus 300 %, elongation at break, electrical resistance and dielectric constant were then characterized. Characterization result showed that (INRL-SC50) with 50/50 ratio irradiated at the dose of 30 kGy, have % conversion of 80.93 %, electrical resistivity of 1.73 x 10"1"4 Ohm cm and dielectric constant of 2.76 which fulfill the requirement as material for electrical gloves. (author)

  4. AcEST: DK958175 [AcEST

    Lifescience Database Archive (English)

    Full Text Available USE RNA-binding protein with multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length = 206 Score = 36...>sp|Q9YGP5|RBPMS_XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms PE=2 SV=1 Lengt... EEVRTLFVSGLPIDIKPRELYLLFRPFKGYE 47 >sp|Q9WVB0|RBPMS_MOUSE RNA-binding protein with multiple splicing OS=Mus musculus GN=Rbpms

  5. Dicty_cDB: Contig-U16596-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .73 1 ( FM992690 ) Candida dubliniensis CD36 chromosome 3, complete ... 48 0.73 1 ( AC137986 ) Medicago trun...10858 |pid:none) Mus musculus N-terminal aceyltrans... 132 3e-29 AY112670_1( AY11...nd RacE (ra... 46 0.12 3 ( EJ551684 ) 1092959454731 Global-Ocean-Sampling_GS-29-0...1-01-1... 48 0.14 2 ( EJ446374 ) 1093015335299 Global-Ocean-Sampling_GS-28-01-01-1... 44 0.18 2 ( EK398314 )... 1095469528885 Global-Ocean-Sampling_GS-31-01-01-1... 50 0.19 1 ( EE263764 ) C01_C01gf4j1_pDNRf_505782 Myzus persicae, li

  6. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA.

    Science.gov (United States)

    Schertzer, Michael; Jouravleva, Karina; Perderiset, Mylene; Dingli, Florent; Loew, Damarys; Le Guen, Tangui; Bardoni, Barbara; de Villartay, Jean-Pierre; Revy, Patrick; Londoño-Vallejo, Arturo

    2015-02-18

    Hoyeraal-Hreidarsson syndrome (HHS) is a severe form of Dyskeratosis congenita characterized by developmental defects, bone marrow failure and immunodeficiency and has been associated with telomere dysfunction. Recently, mutations in Regulator of Telomere ELongation helicase 1 (RTEL1), a helicase first identified in Mus musculus as being responsible for the maintenance of long telomeres, have been identified in several HHS patients. Here we show that RTEL1 is required for the export and the correct cytoplasmic trafficking of the small nuclear (sn) RNA pre-U2, a component of the major spliceosome complex. RTEL1-HHS cells show abnormal subcellular partitioning of pre-U2, defects in the recycling of ribonucleotide proteins (RNP) in the cytoplasm and splicing defects. While most of these phenotypes can be suppressed by re-expressing the wild-type protein in RTEL1-HHS cells, expression of RTEL1 mutated variants in immortalized cells provokes cytoplasmic mislocalizations of pre-U2 and other RNP components, as well as splicing defects, thus phenocopying RTEL1-HHS cellular defects. Strikingly, expression of a cytoplasmic form of RTEL1 is sufficient to correct RNP mislocalizations both in RTEL1-HHS cells and in cells expressing nuclear mutated forms of RTEL1. This work unravels completely unanticipated roles for RTEL1 in RNP trafficking and strongly suggests that defects in RNP biogenesis pathways contribute to the pathology of HHS. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  7. Caregiver Preferences Regarding Personal Health Records in the Management of ADHD.

    Science.gov (United States)

    Ronis, Sarah D; Baldwin, Constance D; McIntosh, Scott; McConnochie, Kenneth; Szilagyi, Peter G; Dolan, James

    2015-07-01

    Personal health records (PHRs) may address the needs of children with attention deficit hyperactivity disorder. Among parents, we assessed acceptance, barriers, and intentions regarding use of PHR for their children with attention deficit hyperactivity disorder. Survey of parents from 3 practices in Rochester, NY. Stepwise logistic regression analysis explored factors predicting respondents' intentions for future use of PHR, accounting for care coordination needs, caregiver education, socioeconomic status, and satisfaction with providers. Of 184 respondents, 23% had used the PHR for their child, 82% intended future use. No difference was observed between users and nonusers regarding gender, age, race, or education. Users were more likely than nonusers to reside in the suburbs (P = .03). Caregivers were more likely to plan future use of the PHR if they felt engaged as partners in their child's care (adjusted odds ratio = 2.3, 95% confidence interval = 1.2, 4.5). Parents are enthusiastic about PHRs. Future work should focus on engaging them as members of the health care team. © The Author(s) 2015.

  8. Sexual selection and the rodent baculum: an intraspecific study in the house mouse (Mus musculus domesticus).

    Science.gov (United States)

    Ramm, Steven A; Khoo, Lin; Stockley, Paula

    2010-01-01

    The rapid divergence of genitalia is a pervasive trend in animal evolution, thought to be due to the action of sexual selection. To test predictions from the sexual selection hypothesis, we here report data on the allometry, variation, plasticity and condition dependence of baculum morphology in the house mouse (Mus musculus domesticus). We find that that baculum size: (a) exhibits no consistent pattern of allometric scaling (baculum size being in most cases unrelated to body size), (b) exhibits low to moderate levels of phenotypic variation, (c) does not exhibit phenotypic plasticity in response to differences in perceived levels of sexual competition and (d) exhibits limited evidence of condition dependence. These patterns provide only limited evidence in support of the sexual selection hypothesis, and no consistent support for any particular sexual selection mechanism; however, more direct measures of how genital morphology influences male fertilization success are required.

  9. Cross-experimental analysis of coat color variations and morphological characteristics of the japanese wild mouse, Mus musculus.

    Science.gov (United States)

    Suzuki, Taichi A; Iwasa, Masahiro A

    2013-01-01

    There are many coat colors in the laboratory mouse, Mus musculus. On the basis of traditional genetics, there are four loci, A-D, related to coat color expressions. As shown by previous studies, Japanese wild mice have gray backs and white bellies and are assumed to carry the A(w) allele at the A (agouti) locus, which is dominant over any other alleles. However, we collected Japanese wild mice from central Honshu with black coats. To understand this black coat expression, we performed cross experiments concerning the four loci using wild-caught mice and DBA/2 laboratory mice from the standpoint of traditional genetics. The offspring of the current crosses showed the wild type, the blackish type, and the intermediate type from some combinations of parents. Considering the coat colors of the offspring, we did not obtain any evidence that the Japanese wild mice always carry the A(w) allele at the A locus. Furthermore, we were not able to explain the current coat color expressions using the traditional logic with regard to the A-D loci and concluded that it is possible for another locus (loci) to be related to the coat color expressions. On the other hand, skull characteristics and external body measurements of the captured wild mice were fundamentally different from those of DBA/2 and offspring from captured wild mice and DBA/2 combinations. Thus, we concluded that the Japanese wild mice had original criteria from a morphological viewpoint.

  10. Toxicological evaluation of essential oil from the leaves of Croton tetradenius (Euphorbiaceae) on Aedes aegypti and Mus musculus.

    Science.gov (United States)

    Carvalho, Karine da Silva; E Silva, Sandra Lúcia da Cunha; de Souza, Ivone Antonia; Gualberto, Simone Andrade; da Cruz, Rômulo Carlos Dantas; Dos Santos, Frances Regiane; de Carvalho, Mário Geraldo

    2016-09-01

    For control of Aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. Herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of Croton tetradenius on Ae. aegypti larvae and adults. We also evaluated the acute toxicity in Mus musculus. The essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. Female mice were used for assessing toxicity according to the Organization for Economic Cooperation and Development's Test Guideline 423/2001. Doses administered to mice orally and intraperitoneally were 5, 50, 300, and 2000 mg kg(-1). There was a greater toxic effect on larvae (LC50 = 0.152 mg mL(-1) and LC90 = 0.297 mg mL(-1)) and on adults (LC50 = 1.842 mg mL(-1) and LC90 = 3.156 mg mL(-1)) of Ae. aegypti after 24 h of exposure, when compared to other periods of exposure. Chemical analysis revealed 26 components, with camphor (25.49 %) as the major component. The acute toxicity via the intraperitoneal route identified an LD50 = 200 mg kg(-1) and by the oral route an LD50 = 500 mg kg(-1). Thus, the essential oil of C. tetradenius presents insecticidal potential for Ae. aegypti and has high safety threshold at the concentrations evaluated in this study.

  11. Dicty_cDB: Contig-U15813-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available purpuratus... 32 4.9 3 ( FL641392 ) TS04-B2 Reticulitermes flavipes symbiont library ... 34 5.0 3 ( FE94079... AC102207 ) Mus musculus chromosome 1, clone RP24-262A17, com... 50 0.41 1 ( EJ558886 ) 1092959528858 Global-Ocean-Sampli...ng_GS-29-01-01-1... 50 0.41 1 ( EJ552305 ) 1092959463211 Global-Ocean-Sampli...) 1095460136297 Global-Ocean-Sampling_GS-31-01-01-1... 48 1.6 1 ( ED538584 ) KBrB132D17F KBrB, Brassica rapa BamHI BAC li...-L03.F SB_BBc Sorghum bicolor genomic 5'... 46 3.4 2 ( EJ602444 ) 1092961206927 Global-Ocean-Sampli

  12. Dicty_cDB: Contig-U04628-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 'e... 44 3.5 1 ( BB649149 ) Mus musculus 16 days embryo head cDNA, RIKEN full... 44 3.5 1 ( GH465909 ) CCUA5...860.b1 CCUA Peromyscus polionotus subgrisieu... 44 3.5 1 ( FG170345 ) AGN_RNC002x

  13. The role of carbon nanotubes in promoting the properties of carbon black-filled natural rubber/butadiene rubber composites

    Directory of Open Access Journals (Sweden)

    Jiangshan Gao

    Full Text Available 80/20 natural rubber (NR/butadiene rubber (BR blends in which the carbon black (CB was replaced partially by multi-walled carbon nanotubes (MWCNTs according to the ratios m (CNTs: m (decreasing amount of CB = 1: X (X was varied from 1 to 6, was prepared by blending of internal mixer and the two-roll mill at the mill opening of 0.5 mm for 10 times. SEM and TEM were used to investigate the filler networks and the good dispersion of fillers. The compounds containing 5 phr CNTs/27.5 phr CB exhibited the best abrasion resistance which was increased by 12.69% compared that without CNTs. 3D morphology images of wear surfaces and tensile fracture surfaces being similar to the layered map of the geography, which match the abrasion resistance and tensile properties, were observed by 3D measuring laser microscope. The uncured blend with 5 phr CNTs/35 phr CB showed the shortest cure time, the highest modulus and level of crosslink density. Significant improvement in mechanical properties were achieved by incorporating 5 phr CNTs and 35 phr CB, and the tear strength, 100% and 300% modulus of the vulcanizate were enhanced by 36.36%, 61.29% and 31.63% compared with the composite with 0 phr CNTs/40 phr CB, respectively. Additionally, compared with the composite without CNTs, the thermal conductivity of the composites with 5 phr CNTs/35 phr CB is increased by an average of 6.15% at three different temperatures. These considerable reinforcements resulted from the synergistic effect of CNTs and CB. Keywords: Synergistic effect, Carbon nanotubes, DIN abrasion, Mechanical properties, Thermal conductivity, 3D measuring laser microscope

  14. NCBI nr-aa BLAST: CBRC-SARA-01-1746 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-1746 ref|NP_705806.1| non-imprinted in Prader-Willi/Angelman syndrome ...1 [Mus musculus] sp|Q8BHK1|NIPA1_MOUSE Non-imprinted in Prader-Willi/Angelman syndrome region protein 1 homo...protein product [Mus musculus] gb|AAH55828.1| Non imprinted in Prader-Willi/Angel...man syndrome 1 homolog (human) [Mus musculus] gb|EDL21870.1| non imprinted in Prader-Willi/Angelman syndrome 1 homolog (human) [Mus musculus] NP_705806.1 1e-113 81% ... ...log gb|AAM34534.1| non-imprinted in Prader-Willi/Angelman syndrome 1 [Mus musculus] dbj|BAC32809.1| unnamed

  15. Efficacy and Palatability of Different Rodenticide Formulations Applied against House Mouse (Mus musculus L. in Plant Storage Facilities

    Directory of Open Access Journals (Sweden)

    Goran Jokić

    2008-01-01

    Full Text Available Palatability (daily intake of different rodenticide formulations based on bromadiolone was compared in experiments with house mouse (Mus musculus L. in agricultural storage facilities, and rodent numbers were assessed at the beginning and end of experiment, as well as rodenticide efficacy. The dynamic of bait intake was monitored for ten days in facilities of the Institute of Animal Husbandry in Zemun and the Agricultural Cooperatives at Starčevo and Omoljica. The experiments complied with the relevant standard method of OEPP/EPPO. Agricultural products were stored either as bulk commodities or in sacs laid on pallete racks in the treated facilities. Baits were laid in boxes on mice routes below palletes holding sacs and on places where significant damage had been observed, at 1-3 m spacing and in 10-20 g portions. Mouse abundance was estimated based on the highest and lowest daily intakes of bait by mice over a period of 10 days, which was divided by the mouse daily feed requirement. The presence of house mouse was also monitored over the next 20 days. The efficacy of test products was computed using Abbott’s formula.

  16. Determination of muscle microcirculation of the limbs of healthy persons and patients with scleroderma by means of /sup 133/Xe clearance. 2. Examination of patients with progressive scleroderma

    Energy Technology Data Exchange (ETDEWEB)

    Poenitzsch, I.; Wiemers, U.; Haustein, U.F.; Schneider, G. (Karl-Marx-Universitaet, Leipzig (German Democratic Republic). Radiologische Klinik)

    1984-01-01

    By means of /sup 133/Xe muscle clearance the blood flow of the musculus tibialis anterior and the musculus opponens pollicis was determined during nonischemic work and after 3 minutes ischemia in patients with progressive scleroderma and additionally of the ischemic musculus opponens pollicis following contrast baths. In relation to 53 patients with normal vessels the reactive hyperemia of the musculus tibialis anterior after ischemia and of the musculus opponens pollicis after heat, cold as well as arterial flow decreasing was significantly decreased in patients with scleroderma and was as a disturbance of the microcirculation realized. For scientific problems in progressive scleroderma the /sup 133/Xe muscle clearance is suitable as to the musculus opponens pollicis.

  17. Dicty_cDB: Contig-U11443-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cal protei... 141 8e-32 BC051184_1( BC051184 |pid:none) Mus musculus USP6 N-terminal like,... 140 1e-31 (Q80...XC3) RecName: Full=USP6 N-terminal-like protein; &AL845275_... 140 1e-31 AL845275...ns clone peg2130 HHL (HH... 140 1e-31 ( Q92738 ) RecName: Full=USP6 N-terminal-like protein; AltName: ...ns cDNA FLJ57209 complet... 110 2e-22 G88391( G88391 )protein R06B10.5 [imported...hnaafngyktvmqllldagadvnshdidfntalhktsftgyhkcaelli ergsqveardnhgitpliksassknfkclsvliergwckcklkr***

  18. Dicty_cDB: Contig-U12550-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available e dilute myosin heavy chain gene for novel heav... 52 5e-10 2 ( DD464188 ) Method for screening pharmaceut...ical candidate sub... 52 5e-10 2 ( AY405224 ) Mus musculus MYO5A gene, VIRTUAL TRAN

  19. Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1)--the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs

    DEFF Research Database (Denmark)

    Krogh, T N; Bachmann, E; Teisner, B

    1997-01-01

    By means of sequence analysis, murine fetal antigen 1 (mFA1) isolated from Mus musculus amniotic fluid was shown to be the circulating protein of the delta-like protein, stromal-cell-derived protein 1 (SCP-1) and preadipocyte factor 1 (Pref-1) gene products. The protein contains 36 cysteine...... residues arranged in six epidermal-growth-factor-like domains. The purification of several C-terminal peptides of varying lengths showed mFA1 to be C-terminal heterogeneous. O-linked glycosylations of the NeuNAc alpha2-3Gal beta1-3(NeuNAc alpha2-6)GalNAc type were present on all C-terminal peptides...... at residues Thr235, Thr244 and Thr248, although glycosylation on Thr244 was only partial. Three N-linked glycosylations were localized in mFA1 (Asn77, Asn142 and Asn151), two of which (Asn142 and Asn151) were in the unusual Asn-Xaa-Cys motif. Fucosylated biantennary complex-type and small amounts (less than 5...

  20. Testing the Electronic Personal Health Record Acceptance Model by Nurses for Managing Their Own Health

    Science.gov (United States)

    Trinkoff, A.M.; Storr, C.L.; Wilson, M.L.; Gurses, A.P.

    2015-01-01

    Summary Background To our knowledge, no evidence is available on health care professionals’ use of electronic personal health records (ePHRs) for their health management. We therefore focused on nurses’ personal use of ePHRs using a modified technology acceptance model. Objectives To examine (1) the psychometric properties of the ePHR acceptance model, (2) the associations of perceived usefulness, ease of use, data privacy and security protection, and perception of self as health-promoting role models to nurses’ own ePHR use, and (3) the moderating influences of age, chronic illness and medication use, and providers’ use of electronic health record (EHRs) on the associations between the ePHR acceptance constructs and ePHR use. Methods A convenience sample of registered nurses, those working in one of 12 hospitals in the Maryland and Washington, DC areas and members of the nursing informatics community (AMIA and HIMSS), were invited to respond to an anonymous online survey; 847 responded. Multiple logistic regression identified associations between the model constructs and ePHR use, and the moderating effect. Results Overall, ePHRs were used by 47%. Sufficient reliability for all scales was found. Three constructs were significantly related to nurses’ own ePHR use after adjusting for covariates: usefulness, data privacy and security protection, and health-promoting role model. Nurses with providers that used EHRs who perceived a higher level of data privacy and security protection had greater odds of ePHR use than those whose providers did not use EHRs. Older nurses with a higher self-perception as health-promoting role models had greater odds of ePHR use than younger nurses. Conclusions Nurses who use ePHRs for their personal health might promote adoption by the general public by serving as health-promoting role models. They can contribute to improvements in patient education and ePHR design, and serve as crucial resources when working with their

  1. The regeneration of thermal wound on mice skin (Mus Musculus) after Q-Switch Nd: YAG laser irradiation for cancer therapy candidate

    Science.gov (United States)

    Apsari, R.; Nahdliyatun, E.; Winarni, D.

    2017-09-01

    The aims of this study are to investigate the regeneration of mice skin tissue (Mus Musculus) irradiated by Q-Switch Nd: YAG laser and morphological change due to Q-Switch Nd: YAG laser irradiation compared to conventional heating (hairdryer). The 2-3 month of twenty-seven mice were used for experimental animals. Mice were incised in the dorsum by the damage effect of laser energy dose (therapeutic dose) of 29.5 J/cm2 with 10 seconds of exposure time, 10 Hz of repetition rate, and 100 pulses of the given single pulse energy. The mice skin tissue was injuried by hairdryer to get burned effect. Mice were divided into three groups, Group I (control) were not treated by anything, Group II were treated by Q-Switch Nd: YAG laser irradiation and sacrificed on (0, 1, 3, 5) days, and Group III were treated by hairdryer then sacrificed on (0, 1, 3, 5) days. Pathology examination showed that the energy of 29,5 J/cm2 dose produced the hole effect (ablation) through the hypodermic layer caused by optical breakdown and collagen coagulation. Thus, the 60 °C temperature of burn showed coagulation necrosis because piknosis discovered in the injured area. The regeneration process showed that the mice skin tissue's ability to regenerate was irradiated by fast laser because of the focus of Q-Switch Nd: YAG laser. It was showed by the scab releases on third day and completely reepithelialization formation on the fifth day. The collagen fibers distribution was same as normal skin tissue on day 5 and so did angiogenesis. Therefore, Q-Switch Nd: YAG laser can be applied for problems of dermatology medical therapies, especially melasma, nevus of ota and tatto therapy. For skin cancer therapy application, energy dose of unregenerated skin tissue is chosen because the death expected effect is permanent.

  2. Relationship between compatibilizer and yield strength of PLA/PP Blend

    Science.gov (United States)

    Jariyakulsith, Pattanun; Puajindanetr, Somchai

    2018-01-01

    The aim of this research is to study the relationship between compatibilizer and yield strength of polylactic acid (PLA) and polypropylene (PP) blend. The PLA is blended with PP (PLA/PP) at the ratios of 70/30, 50/50 and 30/70. In addition, (1) polypropylene grafted maleic anhydride (PP-g-MAH) as a compatibilizer at 0.3 and 0.7 part per hundred of PLA/PP resin (phr) and (2) dicumyl peroxide (DCP) being an initiator at 0.03 and 0.07 phr are added in each composition. Yield strength is characterized to study the interaction between compatibilizer, initiator and yield strength by using experimental design of multilevel full factorial. The results show that (1) the yield strength of PLA/PP blend are increased after addition of compatibilizer. Because the adding of PP-g-MAH and DCP resulted in improving compatibility between PLA and PP. (2) there are interaction between PP-g-MAH and DCP that have affected the final properties of PLA/PP blend. The highest yield strength of 27.68 MPa is provided at the ratio of 70/30 blend by using the 0.3 phr of PP-g-MAH and 0.03 phr of DCP. Linear regression model is fitted and follow the assumptions of normal distribution.

  3. The radioprotective effects of Noni (Morinda citrifolia L.) juice on the bone marrow of irradiated Mus musculus L

    International Nuclear Information System (INIS)

    Boquiren, Ryan Albert Leonard N.; Te Tan, Alvin E.

    2000-03-01

    The radioprotective effects of Noni juice on the bone marrow of irradiated Mus musculus L. (7-week old IRC strain) was studied using the micronucleus test. Five mice were used for each of the six groups; negative and positive control, 25%, 50%, 75%, 100% treatment. Both negative and positive control groups were not treated with Noni juice. However, the negative control was not irradiated. Four different concentrations, a mixture of distilled water and commercial Noni juice, were administered via oral gavage to mice in their specific groups during the 15-day feeding schedule. A day (24H) after the last treatment, the mice were irradiated (6 Gy of gamma radiation). Two days (48H) after irradiation, the mice were sacrifice and the femur were extracted. The bone marrow cells were collected and slides were prepared. One thousand polychromatic erythrocytes (PCE) were screened for the presence of micronucleus per mouse. The mean frequency of micronucleated PCE were as follows: negative control, 15; positive control, 45; 25% treatment, 21; 50% treatment, 18; 75% treatment, 11; 100% treatment, 7. The Kruskal-Wallis test showed that the groups' responses varied (α=0.05). Multiple comparison showed that there were significant differences between: (1) 25% and 100% treatment group; (2) positive control group and 75% treatment group; (3) positive control group and 100% treatment group, thus indicating that Noni juice is effective in inhibiting micronucleus formation at high concentrations. The result also showed that radioprotective potential of Noni juice follows a dose-dependent pattern. (Author)

  4. The radioprotective effects of Noni (Morinda citrifolia L.) juice on the bone marrow of irradiated Mus musculus L

    Energy Technology Data Exchange (ETDEWEB)

    Boquiren, Ryan Albert Leonard N.; Te Tan, Alvin E

    2000-03-01

    The radioprotective effects of Noni juice on the bone marrow of irradiated Mus musculus L. (7-week old IRC strain) was studied using the micronucleus test. Five mice were used for each of the six groups; negative and positive control, 25%, 50%, 75%, 100% treatment. Both negative and positive control groups were not treated with Noni juice. However, the negative control was not irradiated. Four different concentrations, a mixture of distilled water and commercial Noni juice, were administered via oral gavage to mice in their specific groups during the 15-day feeding schedule. A day (24H) after the last treatment, the mice were irradiated (6 Gy of gamma radiation). Two days (48H) after irradiation, the mice were sacrifice and the femur were extracted. The bone marrow cells were collected and slides were prepared. One thousand polychromatic erythrocytes (PCE) were screened for the presence of micronucleus per mouse. The mean frequency of micronucleated PCE were as follows: negative control, 15; positive control, 45; 25% treatment, 21; 50% treatment, 18; 75% treatment, 11; 100% treatment, 7. The Kruskal-Wallis test showed that the groups' responses varied ({alpha}=0.05). Multiple comparison showed that there were significant differences between: (1) 25% and 100% treatment group; (2) positive control group and 75% treatment group; (3) positive control group and 100% treatment group, thus indicating that Noni juice is effective in inhibiting micronucleus formation at high concentrations. The result also showed that radioprotective potential of Noni juice follows a dose-dependent pattern. (Author)

  5. Dicty_cDB: Contig-U03911-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ) lag90e01.y1 Colon epithelia progenitors cDNA Mus ... 64 3e-13 2 ( AV452059 ) Mus musculus cDNA, Abe mouse ES cell cDNA library..... 64 8e-14 2 ( DT212191 ) N124_F10 Non embryogenic SSH library Cichorium in... 46 1e-13 4 ( DJ025875 ) Geno...eatus... 66 7e-12 2 ( AW739394 ) gb41d01.y1 Moss EST library PPN Physcomitrella pa... ...78 1e-11 2 ( BI741051 ) gc93a05.y1 Moss EST library PPN Physcomitrella pa... 78 1...10 2 ( BI741781 ) gc90g06.y1 Moss EST library PPN Physcomitrella pa... 74 2e-10 2 ( BU965247 ) sat08a12.y1 G

  6. Mitochondrial Genetic Diversity and Phylogeography of Mus musculus castaneus in Northern Punjab, Pakistan.

    Science.gov (United States)

    Bibi, Shahnaz; Nadeem, Muhammad Sajid; Wiewel, Andrew Stephen; Beg, Mirza Azhar; Hameed, Khalid; Jabeen, Musarrat; Raja, Ghazala Kaukab

    2017-12-01

    Regions of Iran, Afghanistan, Pakistan and northwestern India have been proposed as the place of origin of Mus musculus castaneus. But despite the fact that Pakistan encompasses an important part of its range, M. m. castaneus populations in Pakistan have not been the subject of intensive genetic and biogeographic studies, except for a very small number of samples included in past studies. We studied genetic variation in M. m. castaneus (CAS) from northern Punjab Province, Pakistan, by using cytochrome b (Cytb) analysis in a sample of 98 individuals. Median-joining network revealed four well differentiated CAS sub-lineages coexisting within a small geographical region; these had previously been thought to have largely non-overlapping geographic distributions. Moreover, haplotypes from Pakistan occupied a central position in the network and all identified global haplotypes were also present in Pakistan. All identified CAS sub-lineages proved to be highly diverse on the basis of haplotype and nucleotide diversity indices. Tajima's D test and Fu's Fs tests of neutrality suggest recent population expansions in all sub-lineages. Expansion times were estimated as 21,760-134,930, 10,800-64,400 and 4950-30,665 ybp using substitution rates of 2.5%, 5% and 11%, respectively. Our results support the hypothesis that northern Punjab Province in Pakistan is the most likely source area for M. m. castaneus, and that the CAS sub-lineages in this region have undergone rapid population expansion events at different time periods, which appear to have benefitted from human-mediated transport, although one of them clearly predates the establishment of human settlements in this region.

  7. AcEST: DK950322 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 19 OS=Mus mus... 34 0.62 >sp|Q9WVB0|RBPMS_MOUSE RNA-binding protein with multiple splicing OS=Mus musculus GN=Rbpms...YGP5|RBPMS_XENLA RNA-binding protein with multiple splicing OS=Xenopus laevis GN=rbpms...ith multiple splicing 2 OS=Mus musculus GN=Rbpms2 PE=1 SV=1 Length = 206 Score = 87.0 bits (214), Expect = 8

  8. Dicty_cDB: Contig-U11740-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cds. 46 3.0 1 ( AB016988 ) Mus musculus mRNA for Nibrin, complete cds. 46 3.0 1 ( DL001650 ) Methods for Molecular Toxicology...eling. 46 3.0 1 ( DD040769 ) Molecular Toxicology Modeling. 46 3.0 1 ( AX401127 ) Sequence 803 from Patent W

  9. Dicty_cDB: Contig-U13205-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |pid:none) Amphimedon queenslandica notch mRN... 50 8e-05 AX805769_1( AX805769 |pid:none) Sequence 1 from P...pid:none) M.musculus mRNA for receptor LR11/gp25... 50 8e-05 EU273942_1( EU273942

  10. THE PATTERN OF THE AGGRESSIVE BEHAVIOR IN THE LABORATORY MOUSE (MUS MUSCULUS

    Directory of Open Access Journals (Sweden)

    Maria BUNEA

    2010-01-01

    Full Text Available Nature has found solutions for decreasing the aggressive impulse, using various inhibitory mechanisms as means of balancing the forces between two fighting individuals, thus avoiding it to lead to the destruction of their own species. These nature's “ pacifyng” solutions are mostly found in animals which are armed with various potentially lethal “weapons”, while in species that live on large territoires and thus have the possibility to avoid conflict, these mechanisms are poorly developed. The purpose of this research is to study the extent of the inhibitory mechanisms against aggression in the laboratory mouse, species in which these mechanisms of diminishing aggression and of avoiding conflicts don't work the same in artificial conditions, where individuals are forced to live in small areas, and to identify the behavioral chains that form the innate manifestations of the aggressive behavior in Mus musculus sp. The results of this study show that in the circumstance of caged individuals, which are crowded so closely together, the intensity of aggression is increasing. The dominance hierarchy is well established after their first fight, but due to the spatial constraint and the fact that there is no place where to flee, the submissive individual oftenly fights back. In every single interraction the aggressive behavior follows a fixed action pattern.

  11. NCBI nr-aa BLAST: CBRC-OPRI-01-1437 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-1437 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blind...Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) [Mus mu...sculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blind...ness, tritan) [Mus musculus] gb|EDL13781.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 1e-162 81% ...

  12. NCBI nr-aa BLAST: CBRC-TTRU-01-0695 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0695 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness...Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) [Mus mu...sculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness..., tritan) [Mus musculus] gb|EDL13781.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 1e-145 80% ...

  13. NCBI nr-aa BLAST: CBRC-MDOM-08-0120 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-08-0120 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness...Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) [Mus mu...sculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness..., tritan) [Mus musculus] gb|EDL13781.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 87% ...

  14. NCBI nr-aa BLAST: CBRC-VPAC-01-1432 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1432 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness...Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) [Mus mu...sculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness..., tritan) [Mus musculus] gb|EDL13781.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 85% ...

  15. NCBI nr-aa BLAST: CBRC-MEUG-01-2797 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-2797 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness...Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan) [Mus mu...sculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness..., tritan) [Mus musculus] gb|EDL13781.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 86% ...

  16. Karakteristik karet ebonit yang dibuat dengan berbagai variasi rasio RSS I/Riklim dan jumlah belerang

    Directory of Open Access Journals (Sweden)

    Penny Setyowati

    2004-07-01

    Full Text Available The aim of the research was to study the influence of utilization of sulphur and reclaim rubber on the characteristics of ebonite rubber, and also to determine the optimum utilization of reclaim rubber and sulphur which yield good physical properties. Ebonite rubber was made by special natural rubber of RSS I (Ribbed Smoke Sheet I mixed with reclaimed rubber at ratio 80/20, 70/30 and 60/40 part hundred rubber/part hundred rubber (phr/phr and utilization of sulphur 30, 40, and 50 phr and also additives respectively. Mixing was done by using open mill, and vulcanization was conducted at early temperature 1200C for 2 hours, and it was continued by post curing at temperature 1500C for 2 hours. The physical properties such as tensile strength, elongation at break, D shore hardness, and izod impact of ebonite rubber vulcanized were determined. The result indicated that characteristics of ebonite rubber was significantly influenced by the amount of added sulphur and 40 phr reclaim rubber or at ratio of RSS I to reclaim rubber 60 to 40 phr/phr. However, the physical properties of ebonite rubber was able to meet the standard quality of group 2 regarding to Classification Hard Rubber issued by ASTM D 2135

  17. Dicty_cDB: Contig-U15610-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 32 1 ( DT767192 ) EST1201041 Aquilegia cDNA library Aquilegia formo... 42 0.040 3 ( EU151142 ) Haemophilus haemolyticu...e... 48 2.0 1 ( ET896158 ) CHO_OF385xi02r1.ab1 CHO_OF Nicotiana tabacum geno... 48 2.0 1 ( EI773383 ) PM1006E24TF BAC library...46 7.9 1 ( AG294250 ) Mus musculus molossinus DNA, clone:MSMg01-070C15.... 46 7.9 1 ( ES370059 ) 5-CP713-021G04 Normalized cDNA libra...03850-501 Normalized CNS library (juven... 42 1.1 2 ( EX859113 ) CBNF4825.rev CBN... 4 ( DU743946 ) ASNC1989.g2 HF10_10-07-02 uncultured marine micro... 38 2.5 3 ( EK037583 ) 1092959478755 Glo

  18. Chromosomal heterozygosity and fertility in house mice (Mus musculus domesticus) from Northern Italy.

    Science.gov (United States)

    Hauffe, H C; Searle, J B

    1998-11-01

    Following the discovery of over 40 Robertsonian (Rb) races of Mus musculus domesticus in Europe and North Africa, the house mouse has been studied extensively as an ideal model to determine the chromosomal changes that may cause or accompany speciation. Current models of chromosomal speciation are based on the assumption that heterozygous individuals have a particularly low fertility, although recent studies indicate otherwise. Despite their importance, fertility estimates for the house mouse are incomplete because traditional measurements, such as anaphase I nondisjunction and germ cell death, are rarely estimated in conjunction with litter size. In an attempt to bridge this gap, we have taken advantage of the house mouse hybrid zone in Upper Valtellina (Lombardy, Italy) in which five Rb races interbreed. We present data on the fertility of naturally occurring ("wild-caught") hybrids and of offspring from laboratory crosses of wild-caught mice ("laboratory-reared"), using various measurements. Wild-caught mice heterozygous for one fusion were more infertile than predicted from past studies, possibly due to genic hybridity; laboratory-reared heterozygotes carrying seven or eight trivalents at meiosis I and heterozygotes carrying one pentavalent also had low fertilities. These low fertilities are especially significant given the probable occurrence of a reinforcement event in Upper Valtellina.

  19. Penelitian Pendahuluan Pengaruh Daun Manggis sebagai Rodentisida Nabati pada Mencit Mus musculus Strain Balepsi

    Directory of Open Access Journals (Sweden)

    Agus Kardinan

    2000-07-01

    Full Text Available Research has been conducted at Pest and Disease Laboratory, Research Institute for Spices and Medicinal Crops, Bogor, during 1997-1998. The objective was to evaluate the possibility of Mangosteen (Garcinia mangostana leaves to be used as botanical rodenticide which is tested to reproduction ability of mice Mus musculus. Eight to ten weeks old mice with the average weight of 25 gram were obtained from Research Institute for Livestock, Ciawi. Research was arranged at randomized block design, 8 treatments and 4 replications. Data was analyzed by determining the average value and their standard deviation values. Observations were done to the weight of testis, weight of embryo, number of embryo, volume of extracts drank by mice and mice behavior during experiment. Result revealed that there was no indication that the leaves extract affected the weight of testis, but it affected the number and weight of embryo. There was indication that mangosteen leaves acted as antifertility on mice. Giving leaves extract increased the total volume of extract drink. There was no abnormality at the activity of mice during experiment, included sex activity, poisonous symptoms and others.

  20. NCBI nr-aa BLAST: CBRC-MMUS-04-0013 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-04-0013 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  1. NCBI nr-aa BLAST: CBRC-PTRO-07-0067 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-07-0067 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  2. NCBI nr-aa BLAST: CBRC-RMAC-04-0050 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-04-0050 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  3. NCBI nr-aa BLAST: CBRC-CFAM-12-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-12-0016 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  4. NCBI nr-aa BLAST: CBRC-ACAR-01-0845 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0845 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  5. NCBI nr-aa BLAST: CBRC-FRUB-02-0074 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0074 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  6. NCBI nr-aa BLAST: CBRC-TBEL-01-1883 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-1883 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  7. NCBI nr-aa BLAST: CBRC-LAFR-01-1734 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1734 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  8. NCBI nr-aa BLAST: CBRC-TNIG-10-0006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-10-0006 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  9. NCBI nr-aa BLAST: CBRC-OCUN-01-1522 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-1522 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  10. NCBI nr-aa BLAST: CBRC-FCAT-01-1020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1020 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  11. NCBI nr-aa BLAST: CBRC-GACU-18-0022 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-18-0022 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  12. NCBI nr-aa BLAST: CBRC-CJAC-01-1332 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1332 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  13. NCBI nr-aa BLAST: CBRC-TNIG-14-0023 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-14-0023 ref|NP_031752.1| cannabinoid receptor 1 (brain) [Mus musculus] sp...|P47746|CNR1_MOUSE Cannabinoid receptor 1 (CB1) (CB-R) (Brain-type cannabinoid receptor) gb|AAD34624.1|AF153345_1 CB1 cann...abinoid receptor [Mus musculus] gb|AAA64413.1| CB1 cannabinoid receptor gb|AAA91176.1| neuronal cann...abinoid receptor emb|CAB42647.1| cannabinoid CB1 receptor [Mus musculus] gb|AAS91800.1| striatal can...nabinoid receptor type 1 protein [Mus musculus] gb|AAS91801.1| striatal cannabinoid

  14. Dicty_cDB: VHC102 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ucing significant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL157766 |pid:none) 1...05 4e-21 BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 2e-20 (Q9JLC8) RecName: Full=Sacsi...n; 103 2e-20 BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 2e-20 CP0015

  15. Pengaruh Ekstrak Daun Sambiloto (Andrographis paniculata terhadap Struktur Mikroanatomi Hepar dan Kadar Glutamat Piruvat Transaminase Serum Mencit (Mus musculus yang Terpapar Diazinon

    Directory of Open Access Journals (Sweden)

    TRI WULANDARI

    2007-11-01

    Full Text Available Diazinon is a pesticide which is often using by farmer to kill insect as theenemy of the plant. The over using of pesticide may result in the remaining of diazinon residue in farming product. This residue can cause the damage of body tissue, especially liver. The aim of research were to find out the effect of leaves sambiloto (Andrographis paniculata Ness. extract on microanatomic structure of liver and serum glutamate pyruvate transaminase (GPT level of mice (Mus musculus L. exposed to diazinon. The research used Compelete Random Design with five treatments. The treatment of each group were using CMC 1% (placebo control, diazinon solution 40 mg/Kg BW (negative ontrol and the leaves sambiloto extract 12,6; 25,2 and 37,8 mg /kg BW. Diazinon solution was given within 10 days and continued with extract of sambiloto leaves also within 10 days. Parameter observed was the microanatomic structure of liver and serum GPT level. The data was analyzed of Analysis of Varians (Anova and continued with DMRT at significance 5%. The result of the research showed that the giving of the extract of sambiloto leaves in some dose variation degree is significantly influential to repair the microanatomic structure of liver and to decrease the serum GPT level was 37,8 mg/Kg BW.

  16. Dicty_cDB: Contig-U16312-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nslandica notch mRN... 70 4e-10 AB001327_1( AB001327 |pid:none) Halocynthia roretzi... 4e-10 AF247637_1( AF247637 |pid:none) Mus musculus LDLR dan mRNA, comple... 70 4e-10 EU273942_1( EU273942 |pid:none) Amphimedon quee

  17. "Pollical palmar interosseous muscle" (musculus adductor pollicis accessorius): attachments, innervation, variations, phylogeny, and implications for human evolution and medicine.

    Science.gov (United States)

    Bello-Hellegouarch, Gaelle; Aziz, M Ashraf; Ferrero, Eva M; Kern, Michael; Francis, Nadia; Diogo, Rui

    2013-03-01

    Most atlases and textbooks dealing with human anatomy do not refer to the "pollical palmar interosseous" (PPI) muscle of Henle. In order to undertake a fresh and detailed study of this muscle and to thus better understand human comparative anatomy and evolution, we: 1) analyze the frequency of the PPI in a large sample of human hands; 2) describe the attachments, innervation and varieties of the PPI in these hands; 3) compare the data obtained with the information available in the literature; and 4) discuss the phylogenetic origin of the PPI and the implications of our observations and comparisons for medicine and for the understanding of human evolutionary history. Within the 72 hands dissected by us, the PPI is present in 67 hands (93%), commonly having a single muscular branch, originating from the medial side of the base of metacarpal I only, inserting onto the medial side of the base of the pollical proximal phalanx and/or surrounding structures (e.g., ulnar sesamoid bone, wing tendon of extensor apparatus), and passing at least partially, and usually mainly, medial to the princeps pollicis artery. A careful study of the human PPI, as well as a detailed comparison with other mammals, strongly suggest that the muscle is evolutionarily derived from the adductor pollicis, and namely from its oblique head. Therefore, we propose that PPI should be designated by the name musculus adductor pollicis accessorius, which indicates that the muscle is most likely a de novo structure derived from the adductor pollicis. Copyright © 2012 Wiley Periodicals, Inc.

  18. Dicty_cDB: VHN647 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ignments: (bits) Value CP001577_292( CP001577 |pid:none) Micromonas sp. RCC299 chromosome... 106 2e-21 (Q9NZJ4) RecName: Full=Sacsi...n; &AL157766_4( AL157766 |pid:none) 105 5e-21 BC138482_1( BC138482 |pid:none) Mus musculus sacsi..... 103 2e-20 (Q9JLC8) RecName: Full=Sacsin; 103 2e-20 BC171956_1( BC171956 |pid:none) Mus musculus sacsi

  19. Iron particle and anisotropic effects on mechanical properties of magneto-sensitive elastomers

    Science.gov (United States)

    Kumar, Vineet; Lee, Dong-Joo

    2017-11-01

    Rubber specimens were prepared by mixing micron-sized iron particles dispersed in room-temperature-vulcanized (RTV) silicone rubber by solution mixing. The possible correlations of the particle volume, size, and distribution with the mechanical properties of the specimens were examined. An isotropic mechanical test shows that at 60 phr, the elastic modulus was 3.29 MPa (electrolyte), 2.92 MPa (carbonyl), and 2.61 MPa (hybrid). The anisotropic effect was examined by curing the specimen under magnetic fields of 0.5-2.0 T at 90° relative to the applied strain. The measurements show anisotropic effects of 11% (carbonyl), 9% (electrolyte), and 6% (hybrid) at 40 phr and 1 T. At 80 phr, the polymer-filler compatibility factor (c-factor) was estimated using the Pythagorean theorem as 0.53 (regular) and 0.73 (anisotropic studies). The improved features could be useful in applications such as controlled damping, vibrational absorption, or automotive bushings.

  20. Pengaruh sistem vulkanisasi konvensional (CV dan semi efisien (SEV terhadap sifat aging dan termal vulkanisat campuran karet alam dan karet butil

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2015-12-01

    Full Text Available The purpose of this research was to study the effect of CV and SEV curing systems on aging and thermal properties of natural rubber and butyl rubber blends. The mixing of rubber and rubber chemicals using two roll-mills and the vulcanization process was carried out by the hydraulic press at 160° C and 150 kg/cm2. The research design used two variations; first, the CV curing system (accelerator/sulfur (1.0/2.5 phr and SEV curing system (accelerator/sulfur (2.1/1.5 phr, second, the ratio of natural rubber/butyl: 100/0; 95/5; 90/10; 85/15; 80/20; 75/25; 70/30; 65/35; 60/40 and 55/45 phr. The parameter observed were tensile strength, elongation at break, hardness before and after aging and thermal properties, e.g.TG/DTA and DSC. The results showed that CV curing systems provided tensile, elongation at break properties gave higher quality and better aging properties. TG/DTA analysis of vulcanizates processed with CV curing systems provided slightly better thermal stability than of the SEV curing system.

  1. Dicty_cDB: VHK810 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available g significant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL157766 |pid:none) 105 5...e-21 BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 2e-20 (Q9JLC8) RecName: Full=Sacsi...n; 103 2e-20 BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA

  2. Fraksi Heksan dan Fraksi Metanol Ekstrak Biji Pepaya Muda Menghambat Spermatogonia Mencit (Mus Musculus Jantan

    Directory of Open Access Journals (Sweden)

    Bagus Komang Satriyasa

    2010-03-01

    Full Text Available To avoid population booming, family planning program should be maintained in which menand woman have the same rights and obligations. However in execution of family planning the roleof men is still low. Studies about contraception in men are limited. Male contraception that isknown in society are condom and vasectomy. In the recent few years in particular, researcheshave been refocused on investigating traditional plants as anti fertility. In use researches manyplants have been studied, including papaya seed. Some researches on herbal anti fertility propertiesof papaya seed have been carried out including those on the active compounds of anti fertility, onapprolaki-lakite dosage of extract of carica papaya seed by animal experiments. Hexane fraction ofunripe papaya seed extract contains glycosides, alkaloid and triterpenoids, which is assumed hasan antifertility effect, so it can be used as a male contraceptive, although the mechanism of actionis not yet clear. Research is conducted at Animal Laboratory Unit Department of PharmacologyFaculty of Medicine University of Udayana, and Laboratorium Patologi Balai Penyidikan danPengujian Veterian (BPPV Wilayah VI Denpasar Direktorat Jenderal Peternakan, DepartmentPertanian. This study used the pre-test and post-test control group design, using 30 male mice ofbalb C strain, aged 12 weeks, weight 20-22 gram, subsequently grouped by random into 3 groupseach consisting of 10 male mice. One control group (P0 = control group was given double distilledwater, and two treatment groups (P1 = treatment group was given fraction of the hexane extractof young Carica papaya seed 20 mg/20gram Body wieght/day, (P2 = treatment group was givenfraction of the methanol extract of young Carica papaya seed 20 mg/20 gram/day. After 36 days oftreatment, evaluation of the testis, of the male mice was conducted. Data were analysed by normalitytest of Shapiro-will, homogeneity test, and Anova test. This study showed that cells of

  3. Dicty_cDB: Contig-U12507-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 129S1/SvImJ Mus musculus STS genomi... 58 0.001 1 ( BV434071 ) S237P6227RB12.T0 PortugueseWaterDog Canis fam...ilia... 58 0.001 1 ( BV424860 ) S237P6196FC6.T0 PortugueseWaterDog Canis familiar

  4. Dicty_cDB: Contig-U14247-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 22555 ) Zebrafish DNA sequence from clone DKEY-19D21 in l... 44 7.5 1 ( BV440136 ) S237P6107RD7.T0 Portugu...eseWaterDog Canis familiar... 44 7.5 1 ( AC158364 ) Mus musculus BAC clone RP23-187

  5. Determination of muscle microcirculation of the limbs of healthy persons and patients with scleroderma by means of 133Xe clearance. 1

    International Nuclear Information System (INIS)

    Wiemers, U.; Poenitzsch, I.; Schneider, G.

    1984-01-01

    By means of 133 Xe muscle clearance the blood flow of the musculus tibialis anterior and the musculus opponens pollicis was determined during nonischemic work and also after 3 minutes ischemia in 53 patients with healthy vessels. Additionally 133 Xe muscle clearance of the musculus opponens pollicis after influence of heat, cold as well as arterial flow reduction. The flow values after ischemia were higher than during nonischemic work. In relation to the functional test the reactive hyperemia after 3 minutes ischemia proved to be more favorable in realization and standardization. In persons under the age of 30 little increased flow values of the musculus tibialis anterior compared to persons over 30 were determined. (author)

  6. Dicty_cDB: Contig-U13401-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available musculus c... 139 2e-31 AY651828_5( AY651828 |pid:none) Cotesia plutellae polydnavirus seg... 139 2e-31 AK30... 1e-29 AY651829_10( AY651829 |pid:none) Cotesia plutellae polydnavirus se... 132 1e-29 Z36237_5( Z36237 |pid...K294673_1( AK294673 |pid:none) Homo sapiens cDNA FLJ59320 complet... 127 6e-28 EF067328_9( EF067328 |pid:none) Cotesia plutella..._1( U20807 |pid:none) Bos taurus protein tyrosine phosphatas... 127 8e-28 AY651829_1( AY651829 |pid:none) Cotesia plutella...sph... 126 1e-27 EF067324_5( EF067324 |pid:none) Cotesia plutellae polydnavirus seg... 126 1e-27 AK064263_1(

  7. Sugarcane bagasse derivative-based superabsorbent containing phosphate rock with water-fertilizer integration.

    Science.gov (United States)

    Zhong, Kang; Zheng, Xi-Liang; Mao, Xiao-Yun; Lin, Zuan-Tao; Jiang, Gang-Biao

    2012-10-01

    To improve the water-fertilizer utilization ratio and mitigate the environmental contamination, an eco-friendly superabsorbent polymer (SPA), modified sugarcane bagasse/poly (acrylic acid) embedding phosphate rock (MSB/PAA/PHR), was prepared. Ammonia, phosphate rock (PHR) and KOH were admixed in the presence of acrylic acid to provide nitrogen (N), phosphorus (P) and potassium (K) nutrients, respectively. Impacts on water absorption capacity of the superabsorbent polymer (SAP) were investigated. The maximum swelling capacity in distilled water and 0.9 wt.% (weight percent) NaCl solution reached 414 gg(-1) and 55 gg(-1) (water/prepared SAP), respectively. The available NPK contents of the combination system were 15.13 mgg(-1), 6.93 mgg(-1) and 52.05 mgg(-1), respectively. Moreover, the release behaviors of NPK in the MSB/PAA/PHR were also studied. The results showed that the MSB/PAA/PHR has outstanding sustained-release plant nutrients property. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. No evidence for punishment in communally nursing female house mice (Mus musculus domesticus).

    Science.gov (United States)

    Ferrari, Manuela; König, Barbara

    2017-01-01

    Punishment is claimed as an important mechanism to stabilise costly cooperation in humans, but its importance in social animals has been questioned recently due to both conceptual considerations and a lack of empirical evidence (only few published studies). We empirically tested whether there is evidence for punishment in communally nursing house mice (Mus musculus domesticus, direct descendants of "wild" animals). Communally breeding females pool their litters and raise all offspring together, indiscriminately caring for own and other offspring. Such a situation resembles a public good and provides scope for exploitation if females vary in their relative contributions to the joint nest (offspring number). We allowed two females to communally breed and conducted removal experiments both in the presence and absence of pups. We aimed to test whether reduced investment by one of the females (induced through separation from the partner and their combined offspring for 4 or 12 hours) leads to increased aggression by the other female after the reunion. We found no evidence for punishment, on the contrary, females increased socio-positive behaviours. The costs of losing a partner in a communally breeding species might be too high and hinder the evolution of punishment. Our findings add to a growing list of examples questioning the role of punishment in cooperating non-human animals and emphasise the importance of empirical testing of its assumptions and predictions.

  9. Dicty_cDB: Contig-U05216-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 064701 ) WNEL14b1 Wheat EST endosperm library Triticum aes... 40 0.032 2 ( AC200123 ) Zea mays chromosome 4 ... CF-24-HW fat cDNA... 36 0.055 2 ( EG551033 ) MM04K05_RP Sugar Beet germination cDNA library Be... 36 0.055 2 ( AG332587 ) Mus muscul...7 2 ( AZ506962 ) 1M0348D20F Mouse 10kb plasmid UUGC1M library Mus ... 40 0.057 2 ( BB898919 ) Macaca fascicul...V968176 ) GC06167 Gracilaria changii cDNA library Gracilari... 46 0.014 2 ( AG430324 ) Mus musculus molossin..._IpSto_12_p10 Stomach cDNA library Ictalurus p... 32 0.76 2 ( DX535456 ) GH_MBb0065G22f GH_MBb Gossypium hirsutum genomic

  10. NCBI nr-aa BLAST: CBRC-MMUS-06-0131 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0131 ref|NP_444459.1| vomeronasal 1 receptor, B8 [Mus musculus] sp|Q9EQ45|VN1B8_MOUSE Vomeronas...al type-1 receptor B8 (Vomeronasal type-1 receptor A13) gb|AAG42088.1|AF291494_1 vomeronasa...l receptor V1RB8 [Mus musculus] dbj|BAB79220.1| vomeronasal receptor 1 A13 [Mus musculus] gb|AAI40252.1| Vomeronasa...l 1 receptor, B8 [synthetic construct] gb|EDK99274.1| vomeronasal ...1 receptor, B8 [Mus musculus] gb|AAI46525.1| Vomeronasal 1 receptor, B8 [synthetic construct] NP_444459.1 1e-175 100% ...

  11. AcEST: BP921475 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BlastX Result : Swiss-Prot sp_hit_id O35902 Definition sp|O35902|DSG3_MOUSE Desmoglein-3 OS=Mus musculus Al...gnificant alignments: (bits) Value sp|O35902|DSG3_MOUSE Desmoglein-3 OS=Mus musculus GN=Dsg3 PE=2 SV=1 31 2....2 sp|Q29IL2|POMT2_DROPS Protein O-mannosyl-transferase 2 OS=Drosop... 30 4.8 >sp|O35902|DSG3_MOUSE Desmog

  12. Dicty_cDB: Contig-U12092-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 446 |pid:none) Shewanella sp. MR-4, complete g... 47 9e-04 (Q5NC05) RecName: Full=Transcription term... beige protein homolog (l... 40 4.4 2 ( EK325198 ) 1095467004583 Global-Ocean-Sampling_GS-31-01-01-1... 34 5....0 2 ( AC154124 ) Mus musculus chromosome 5, clone RP24-63G13, comp... 40 5.7 5 ( EJ839652 ) 1093017822861 Global-Ocean-Sampli...om clone RP23-266C4 on chrom... 36 6.3 4 ( EJ354365 ) 1092963621964 Global-Ocean-Sampling_GS-28-01-01-1... 3...|pid:none) Streptomyces echinatus aranciamyc... 50 6e-05 A84014( A84014 ) SNF2 helicase BH2913 [imported] -

  13. A survey on helminthic infection in mice (Mus musculus and rats (Rattus norvegicus and Rattus rattus in Kermanshah, Iran

    Directory of Open Access Journals (Sweden)

    Norollah Pakdel

    2013-06-01

    Full Text Available Parasitic infections of rodents can compromise scientific research as well as the health of the animals and humans. Based on previous studies, infection rate of parasitic helminths is different in various regions of Iran. The current survey was aimed to determine endoparasitic helminths infection in 138 trapped rodents of Kermanshah county, Iran. Mice and rats were trapped using metal snares from January to October 2011 and euthanized. Rodents included 110 Mus musculus (79.00%, 23 Rattus norvegicus (17.00%, and five Rattus rattus (4.00%. The gastrointestinal and respiratory tracts were removed and examined to identify parasitic helminths. The results indicated that 42.02% of examined rodents were infected with eight helminths species, i.e. Trichuris muris (14.49%, Syphacia obvelata (13.76%, Syphacia muris (2.89%, Aspicularis tetrapetra (5.07%, Heterakis spumosa (5.07%, Capillaria hepatica eggs (3.62%, Hyminolepis diminuta (12.30%, and Cystisercus fasciolaris, the larva of Taenia teanieformis (4.34%. Given the results of this study, we concluded that examined rodents were more infected with nematodes than other helminths. As rodents are usually infected with a number of zoonotic parasites, hence control of these animals has an important role in safeguarding public health.

  14. Dicty_cDB: Contig-U15590-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available -20 CT005272_460( CT005272 |pid:none) Leishmania major strain Friedlin... 85 7e-20 S37963( S37963 ;S47957) mitochondrial intermed...D6) RecName: Full=Mitochondrial intermediate peptidase; ... 89 5e-34 CP000964_2806( CP000964 |pid:none) Klebsie... U80034_1( U80034 |pid:none) Homo sapiens mitochondrial intermediat... 93 4e-31 AY075581_1( AY075581 |pid:no...95 2e-17 AK050120_1( AK050120 |pid:none) Mus musculus adult male liver tumo... 91 3e-16 (Q753X4) RecName: Full=Mitochondrial interm... (A7TSL2) RecName: Full=Mitochondrial intermediate peptidase; ... 101 3e-34 (Q6CH

  15. Silane grafted natural rubber and its compatibilization effect on silica-reinforced rubber tire compounds

    NARCIS (Netherlands)

    Sengloyluan, K.; Sahakaro, K.; Dierkes, W. K.; Noordermeer, J. W.M.

    2017-01-01

    Natural Rubber (NR) grafted with 3-octanoylthio-1-propyltriethoxysilane (NXT) was prepared by melt mixing using 1,1′-di(tert-butylperoxy)-3,3,5-trimethylcyclohexane as initiator at 140 °C with NXT contents of 10 and 20 parts per hundred rubber [phr] and initiator 0.1 phr. The silane grafted on NR

  16. Dicty_cDB: Contig-U10913-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .013 ( Q92738 ) RecName: Full=USP6 N-terminal-like protein; AltName: Fu... 44 0.013 D13644_1( D13644 |pid:none) Homo sapie...4 2 ( AC090393 ) Homo sapiens chromosome 18, clone RP11-738B2, com... 36 2.7 7 ( ER548332 ) 1093016199934 Global-Ocean-Sampli...288 pro... 44 0.012 BC051184_1( BC051184 |pid:none) Mus musculus USP6 N-terminal like,... 44 0.013 AK099868_... BT038351 |pid:none) Zea mays full-length cDNA clone ZM... 44 0.013 (Q80XC3) RecName: Full=USP6 N-terminal-li...095506062409 Global-Ocean-Sampling_GS-31-01-01-1... 48 0.83 1 ( CC504594 ) CH240_344F23.TARBAC13P2 CHORI-240

  17. Characterizations of Polystyrene-Based Hybrid Particles Containing Hydrophobic Mg(OH2 Powder and Composites Fabricated by Employing Resultant Hybrid Particles

    Directory of Open Access Journals (Sweden)

    Shuichi Kimura

    2007-01-01

    unchanged, even when the ST-1 powder content increased from 10 to 50 phr. Furthermore, a composite fabricated by employing the hybrid particles achieved homogenous distribution of ST-1 powder and showed a higher oxygen index than that of a composite fabricated by directly mixing of PS pellets and ST-1 powder.

  18. WOUND HEALING ACTIVITY OF UNGUENTUM DOSAGE FORM OF ETHANOLIC EXTRACTS OF Areca catechu L. NUT IN Mus musculus albinus

    Directory of Open Access Journals (Sweden)

    Azizah Vonna

    2015-10-01

    Full Text Available The activity test of ethanol extract of betel nut ointment (Areca catechu L. in wound healing on mice (Mus musculus albinus has been carried out to determine the ability of the ethanol extract of betel nut ointment in wound healing and determine the concentration which was accelerate the wound healing on mice between 2 concentrations. This experimental research method used completely randomized design (CRD using 20 mices divided into 4 treatment groups ; ointment base, povidone iodine ointment, ethanol extract of betel nut ointment (SEEBP 2% and SEEBP 4%. Each treatment groups was tested in the incision which was made along the 15 mm parallel to the spine (Os. Vetebre with the depth until subcutaneous skin layers. The ointment was applied twice a day for about 21 days and observed changes every day for during the period of observation. The results showed that the average length of time of the scab formation, the scab exfoliation, and the wound healing successively are for the ointment base was 6.6; 10.2 and 18.2 days, povidone iodine ointment was 7; 11.2 and 14.8 days, SEEBP 2% was 5.75; 7.75 and 13.25 days, SEEBP 4% was 4.2; 8.8 and 12.8 days. ANOVA and LSD results of scab formation time showed a significant difference between SEEBP 4% with base ointment and povidone iodine ointment (p <0.05. Results of the exfoliation scab showed a significance difference between SEEBP 2% with base ointment and povidone iodine ointment (p <0.05. The duration of wound healing showed that there was significance difference between SEEBP 2%, SEBP 4% and povidone iodine ointment with ointment base  (p<0.05.Thus, betel nut ointment as an effect on healing process. The concentration which can accelerate wound healing in mice is SEEBP 4%.

  19. Penelitian “polymer blend” high styrene rubber dengan karet alam untuk “sponnge middle sole”

    Directory of Open Access Journals (Sweden)

    Penny Setyowati

    1999-07-01

    Full Text Available The objective of this research was find optimum composision of polymer blend of High Styrene Rubber (HSR and Natural Rubber (SIR 3L for preparation sponge middle sole that save the best fisical properties, strong, flexible, light, stable and thermoplastic properties. The formulation of rubber compound has to be researched as much as 3 formalition MS 1, MS II and MS III with variation of SIR 3L, HSR and HAF black as follows, MS I 70-30-20 phr ; MS II 80-20-30 PHR and MS III 90-10-40 phr . From the fisical test analysis, optimum formulation to be reached at the compound formualtion MS III with result of fisical test as follows : Tensile strength : 0.572 kg/mm2 ; Elongation at break : 204% ; Hardness share A : 55,8 : Density 0,728 gr/cm3 : Tear Resistance : 0,296 kg/mm2 and Compression Set 22,042%.

  20. Consumer attitudes toward personal health records in a beacon community.

    Science.gov (United States)

    Patel, Vaishali N; Abramson, Erika; Edwards, Alison M; Cheung, Melissa A; Dhopeshwarkar, Rina V; Kaushal, Rainu

    2011-04-01

    To characterize consumers' attitudes about personal health records (PHRs), electronic tools that enable consumers to securely access, manage, and share their health information, in a community participating in health information technology initiatives. Cross-sectional study. A random-digit-dial telephone survey about PHRs was conducted among adult residents of New York State's greater Buffalo region. Multivariate regression analyses identified factors associated with potential PHR use. We obtained a 79% (n = 200) response rate. Many respondents (70%) would potentially use PHRs. Consumers wanted PHRs to incorporate an array of information, including immunization records (89%) and providers visited (88%). They expressed interest in several online activities, including accessing their family members' healthcare information (71%). Potential PHR use was associated with perceptions that PHRs would improve privacy and security of medical information (odds ratio [OR] 4.7; 95% confidence interval [CI] 1.1, 20.1), understanding regarding health (OR 3.7; 95% CI 1.3, 11.1), and overall quality of care (OR 3.6; 95% CI 1.2, 10.6). Potential PHR use was associated with annual household income of more than $30,000 (OR 3.9; 95% CI 1.3, 11.9) and experience looking up health information online (OR 3.0; 95% CI 1.1, 8.1). Consumers expressed great interest in using PHRs and wanted comprehensive PHRs. However, the "digital divide" between those with varying levels of Internet experience and concerns about PHRs’ effect on privacy and security of medical information may limit use. Designing PHRs that incorporate consumer preferences and developing policies that address these barriers may increase consumers' PHR use.

  1. The electrostatic role of the Zn-Cys2His2 complex in binding of operator DNA with transcription factors: mouse EGR-1 from the Cys2His2 family.

    Science.gov (United States)

    Chirgadze, Y N; Boshkova, E A; Polozov, R V; Sivozhelezov, V S; Dzyabchenko, A V; Kuzminsky, M B; Stepanenko, V A; Ivanov, V V

    2018-01-07

    The mouse factor Zif268, known also as early growth response protein EGR-1, is a classical representative for the Cys2His2 transcription factor family. It is required for binding the RNA polymerase with operator dsDNA to initialize the transcription process. We have shown that only in this family of total six Zn-finger protein families the Zn complex plays a significant role in the protein-DNA binding. Electrostatic feature of this complex in the binding of factor Zif268 from Mus musculus with operator DNA has been considered. The factor consists of three similar Zn-finger units which bind with triplets of coding DNA. Essential contacts of the factor with the DNA phosphates are formed by three conservative His residues, one in each finger. We describe here the results of calculations of the electrostatic potentials for the Zn-Cys2His2 complex, Zn-finger unit 1, and the whole transcription factor. The potential of Zif268 has a positive area on the factor surface, and it corresponds exactly to the binding sites of each of Zn-finger units. The main part of these areas is determined by conservative His residues, which form contacts with the DNA phosphate groups. Our result shows that the electrostatic positive potential of this histidine residue is enhanced due to the Zn complex. The other contacts of the Zn-finger with DNA are related to nucleotide bases, and they are responsible for the sequence-specific binding with DNA. This result may be extended to all other members of the Cys2His2 transcription factor family.

  2. Dicty_cDB: VHO809 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available e E Sequences producing significant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL1...57766 |pid:none) 105 5e-21 BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 1e-20 B...C138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 2e-20 (Q9JLC8) RecName: Full=Sacsin

  3. Dicty_cDB: VHO533 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available producing significant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL...157766_4( AL157766 |pid:none) 96 2e-18 BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl...... 94 8e-18 (Q9JLC8) RecName: Full=Sacsin; 94 8e-18 BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA

  4. PRDM9 drives evolutionary erosion of hotspots in Mus musculus through haplotype-specific initiation of meiotic recombination.

    Science.gov (United States)

    Baker, Christopher L; Kajita, Shimpei; Walker, Michael; Saxl, Ruth L; Raghupathy, Narayanan; Choi, Kwangbom; Petkov, Petko M; Paigen, Kenneth

    2015-01-01

    Meiotic recombination generates new genetic variation and assures the proper segregation of chromosomes in gametes. PRDM9, a zinc finger protein with histone methyltransferase activity, initiates meiotic recombination by binding DNA at recombination hotspots and directing the position of DNA double-strand breaks (DSB). The DSB repair mechanism suggests that hotspots should eventually self-destruct, yet genome-wide recombination levels remain constant, a conundrum known as the hotspot paradox. To test if PRDM9 drives this evolutionary erosion, we measured activity of the Prdm9Cst allele in two Mus musculus subspecies, M.m. castaneus, in which Prdm9Cst arose, and M.m. domesticus, into which Prdm9Cst was introduced experimentally. Comparing these two strains, we find that haplotype differences at hotspots lead to qualitative and quantitative changes in PRDM9 binding and activity. Using Mus spretus as an outlier, we found most variants affecting PRDM9Cst binding arose and were fixed in M.m. castaneus, suppressing hotspot activity. Furthermore, M.m. castaneus×M.m. domesticus F1 hybrids exhibit novel hotspots, with large haplotype biases in both PRDM9 binding and chromatin modification. These novel hotspots represent sites of historic evolutionary erosion that become activated in hybrids due to crosstalk between one parent's Prdm9 allele and the opposite parent's chromosome. Together these data support a model where haplotype-specific PRDM9 binding directs biased gene conversion at hotspots, ultimately leading to hotspot erosion.

  5. Dicty_cDB: Contig-U10011-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 38 0.082 2 ( AC018940 ) Homo sapiens clone RP11-22A2, WORKING DRAFT SEQUE... 44 0.088 3 ( EJ460859 ) 1093018538988 Global-Ocean-Sampl...in family member 1; AltName: Ful... 72 3e-11 ( Q92738 ) RecName: Full=USP6 N-terminal-like protein; AltName:...chromos... 68 4e-10 BC051184_1( BC051184 |pid:none) Mus musculus USP6 N-terminal li...ke,... 68 4e-10 (A6H8I2) RecName: Full=Small G protein signaling modulator 3 hom... 68 4e-10 (Q80XC3) RecName: Full=USP6 N-term...Plasmodium berghei cDNA clone:LV000110, liver sta... 38 0.33 2 ( AC006287 ) Homo sapiens, clone hRPK.22_A_1, compl

  6. Are personal health records safe? A review of free web-accessible personal health record privacy policies.

    Science.gov (United States)

    Carrión Señor, Inmaculada; Fernández-Alemán, José Luis; Toval, Ambrosio

    2012-08-23

    Several obstacles prevent the adoption and use of personal health record (PHR) systems, including users' concerns regarding the privacy and security of their personal health information. To analyze the privacy and security characteristics of PHR privacy policies. It is hoped that identification of the strengths and weaknesses of the PHR systems will be useful for PHR users, health care professionals, decision makers, and designers. We conducted a systematic review using the principal databases related to health and computer science to discover the Web-based and free PHR systems mentioned in published articles. The privacy policy of each PHR system selected was reviewed to extract its main privacy and security characteristics. The search of databases and the myPHR website provided a total of 52 PHR systems, of which 24 met our inclusion criteria. Of these, 17 (71%) allowed users to manage their data and to control access to their health care information. Only 9 (38%) PHR systems permitted users to check who had accessed their data. The majority of PHR systems used information related to the users' accesses to monitor and analyze system use, 12 (50%) of them aggregated user information to publish trends, and 20 (83%) used diverse types of security measures. Finally, 15 (63%) PHR systems were based on regulations or principles such as the US Health Insurance Portability and Accountability Act (HIPAA) and the Health on the Net Foundation Code of Conduct (HONcode). Most privacy policies of PHR systems do not provide an in-depth description of the security measures that they use. Moreover, compliance with standards and regulations in PHR systems is still low.

  7. Human-centered design of a personal health record system for metabolic syndrome management based on the ISO 9241-210:2010 standard

    Directory of Open Access Journals (Sweden)

    Farinango CD

    2018-01-01

    Full Text Available Charic D Farinango,1 Juan S Benavides,1 Jesús D Cerón,1 Diego M López,1 Rosa E Álvarez2 1Telematics Engineering Research Group, Faculty of Electronics and Telecommunications Engineering, Universidad del Cauca, Popayán, Colombia; 2Human Genetics Research Group, Faculty of Health Sciences, Universidad del Cauca, Popayán, Colombia Background: Previous studies have demonstrated the effectiveness of information and communication technologies to support healthy lifestyle interventions. In particular, personal health record systems (PHR-Ss empower self-care, essential to support lifestyle changes. Approaches such as the user-centered design (UCD, which is already a standard within the software industry (ISO 9241-210:2010, provide specifications and guidelines to guarantee user acceptance and quality of eHealth systems. However, no single PHR-S for metabolic syndrome (MS developed following the recommendations of the ISO 9241-210:2010 specification has been found in the literature.Objective: The aim of this study was to describe the development of a PHR-S for the management of MS according to the principles and recommendations of the ISO 9241-210 standard.Methods: The proposed PHR-S was developed using a formal software development process which, in addition to the traditional activities of any software process, included the principles and recommendations of the ISO 9241-210 standard. To gather user information, a survey sample of 1,187 individuals, eight interviews, and a focus group with seven people were performed. Throughout five iterations, three prototypes were built. Potential users of each system evaluated each prototype. The quality attributes of efficiency, effectiveness, and user satisfaction were assessed using metrics defined in the ISO/IEC 25022 standard.Results: The following results were obtained: 1 a technology profile from 1,187 ­individuals at risk for MS from the city of Popayan, Colombia, identifying that 75.2% of the people

  8. Sociotechnical Challenges of Developing an Interoperable Personal Health Record

    Science.gov (United States)

    Gaskin, G.L.; Longhurst, C.A.; Slayton, R.; Das, A.K.

    2011-01-01

    Objectives To analyze sociotechnical issues involved in the process of developing an interoperable commercial Personal Health Record (PHR) in a hospital setting, and to create guidelines for future PHR implementations. Methods This qualitative study utilized observational research and semi-structured interviews with 8 members of the hospital team, as gathered over a 28 week period of developing and adapting a vendor-based PHR at Lucile Packard Children’s Hospital at Stanford University. A grounded theory approach was utilized to code and analyze over 100 pages of typewritten field notes and interview transcripts. This grounded analysis allowed themes to surface during the data collection process which were subsequently explored in greater detail in the observations and interviews. Results Four major themes emerged: (1) Multidisciplinary teamwork helped team members identify crucial features of the PHR; (2) Divergent goals for the PHR existed even within the hospital team; (3) Differing organizational conceptions of the end-user between the hospital and software company differentially shaped expectations for the final product; (4) Difficulties with coordination and accountability between the hospital and software company caused major delays and expenses and strained the relationship between hospital and software vendor. Conclusions Though commercial interoperable PHRs have great potential to improve healthcare, the process of designing and developing such systems is an inherently sociotechnical process with many complex issues and barriers. This paper offers recommendations based on the lessons learned to guide future development of such PHRs. PMID:22003373

  9. NCBI nr-aa BLAST: CBRC-OGAR-01-1096 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OGAR-01-1096 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindnes...s, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 1e-105 63% ...

  10. NCBI nr-aa BLAST: CBRC-LAFR-01-3136 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-3136 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindnes...s, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 1e-152 85% ...

  11. NCBI nr-aa BLAST: CBRC-CPOR-01-1188 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-1188 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindnes...s, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 1e-151 75% ...

  12. Hypoxia triggers short term potentiation of phrenic motoneuron discharge after chronic cervical spinal cord injury

    Science.gov (United States)

    Lee, Kun-Ze; Sandhu, Milapjit S.; Dougherty, Brendan J.; Reier, Paul J.; Fuller, David D.

    2014-01-01

    Repeated exposure to hypoxia can induce spinal neuroplasticity as well as respiratory and somatic motor recovery after spinal cord injury (SCI). The purpose of the present study was to define the capacity for a single bout of hypoxia to trigger short-term plasticity in phrenic output after cervical SCI, and to determine the phrenic motoneuron (PhrMN) bursting and recruitment patterns underlying the response. Hypoxia-induced short term potentiation (STP) of phrenic motor output was quantified in anesthetized rats 11 wks following lateral spinal hemisection at C2 (C2Hx). A 3-min hypoxic episode (12–14% O2) always triggered STP of inspiratory burst amplitude, the magnitude of which was greater in phrenic bursting ipsilateral vs. contralateral to C2Hx. We next determined if STP could be evoked in recruited (silent) PhrMNs ipsilateral to C2Hx. Individual PhrMN action potentials were recorded during and following hypoxia using a “single fiber” approach. STP of bursting activity did not occur in cells initiating bursting at inspiratory onset, but was robust in recruited PhrMNs as well as previously active cells initiating bursting later in the inspiratory effort. We conclude that following chronic C2Hx, a single bout of hypoxia triggers recruitment of PhrMNs in the ipsilateral spinal cord with bursting that persists beyond the hypoxic exposure. The results provide further support for the use of short bouts of hypoxia as a neurorehabilitative training modality following SCI. PMID:25448009

  13. Determination of muscle microcirculation of the limbs of healthy persons and patients with scleroderma by means of /sup 133/Xe clearance. 1. Examination of patients with healthy vessels

    Energy Technology Data Exchange (ETDEWEB)

    Wiemers, U.; Poenitzsch, I.; Schneider, G. (Karl-Marx-Universitaet, Leipzig (German Democratic Republic). Radiologische Klinik)

    1984-01-01

    By means of /sup 133/Xe muscle clearance the blood flow of the musculus tibialis anterior and the musculus opponens pollicis was determined during nonischemic work and also after 3 minutes ischemia in 53 patients with healthy vessels. Additionally /sup 133/Xe muscle clearance of the musculus opponens pollicis after influence of heat, cold as well as arterial flow reduction. The flow values after ischemia were higher than during nonischemic work. In relation to the functional test the reactive hyperemia after 3 minutes ischemia proved to be more favorable in realization and standardization. In persons under the age of 30 little increased flow values of the musculus tibialis anterior compared to persons over 30 were determined.

  14. Dicty_cDB: VHF491 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ents: (bits) Value BC142916_1( BC142916 |pid:none) Danio rerio hypothetical LOC555303... 97 6e-19 (Q9NZJ4) RecName: Full=Sacsi...n; &AL157766_4( AL157766 |pid:none) 97 9e-19 BC171956_1( BC171956 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 96 1e-18 BC138482_1( BC138482 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 96 2e-18 (Q9JLC8) RecName: Full=Sacsin; 96 2e-18 CP001577_292( CP001577 |pid:none) Mi

  15. NCBI nr-aa BLAST: CBRC-MMUS-06-0014 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0014 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blind... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blind...ness, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blind...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 100% ...

  16. NCBI nr-aa BLAST: CBRC-BTAU-01-2837 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2837 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindnes...s, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 86% ...

  17. NCBI nr-aa BLAST: CBRC-RNOR-04-0072 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0072 ref|NP_031564.1| opsin 1 (cone pigments), short-wave-sensitive (color blindness... pigment gb|AAB53320.1| blue cone pigment [Mus musculus] gb|AAH26021.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindnes...s, tritan) [Mus musculus] gb|AAH58267.1| Opsin 1 (cone pigments), short-wave-sensitive (color blindness...s), short-wave-sensitive (color blindness, tritan), isoform CRA_a [Mus musculus] NP_031564.1 0.0 95% ...

  18. Pengaruh penggunaan nitril butadiene rubber dan pale crepe pada pembuatan sol karet untuk sepatu pengaman

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2010-06-01

    Full Text Available Abstract Rubber sole for safety shoes was different on physical specification with general sole, especially on abrasion resistance and oil resistance. The objective of the study was to determine the effect of nitril butadiene rubber and pale crepe on physical properties of vulcanized rubber sole for safety shoes. Rubber sole for safety shoes was produced by blending pale crepe and nitril butadiene rubber with ratio of : 50/50; 60/40; 70/30 and 80/20 phr, respectively. Carbon black as filler was also variated with, 40 ; 50 and 60 phr. Compounding processing used two roll mill machine and vulcanized rubber sole was by using pressed use hydraulic press machine. The results showed that vulcanized rubber sole for safety shoes with good quality consist of pale crepe and NBR 80/20 phr and carbon black 40 phr, which was characterized by tensile strength 16.81 N/mm2, tear strength 11.68 N/mm, density 1.12 g/cm3, abrasion resistance 58.51 mm3, hardness 71.60 shore A, resistance to cut growth 30.000 times was 1.15 mm and oil resistance 65.44%, respectively. The quality parameters was complied with standard quality of SNI 0111 : 2009, for safety shoes from leather and vulcanized rubber sole that fulfill oil reistance parameter.

  19. Effect of maleated natural rubber on tensile strength and compatibility of natural rubber/coconut coir composite

    Science.gov (United States)

    Ujianto, O.; Noviyanti, R.; Wijaya, R.; Ramadhoni, B.

    2017-07-01

    Natural rubber (NR)/coconut coir (CF) composites were fabricated using co-rotating twin screw extruder with maleated NR (MNR) used as compatibilizer. The MNR was produced at three level of maleic anhydride (MA), and analyzed qualitative and quantitatively using FTIR and titration technique. Analysis on MNR using FTIR and titration methods showed that MA was grafted on NR chain at different percentage (0.76, 2.23, 4.79%) depended on MA concentration. Tensile strength data showed the best tensile strength was produced at 7 phr of MNR with 1 phr of MA level in MNR resulting 16.4 MPa. The improvement of compatibilized samples were more than 300% compare to uncompatibilized composite attributed to better interfacial bonding. The improvement on tensile strength was significantly influenced by MNR level and amount of MA added to produce MNR, as well as their interaction. The optimum conditions for producing NR-CF composite were predicted at 6.5 phr of MNR level with 1 phr of MA concentration added in MNR production, regardless screw rotation settings. Results from verification experiments confirm that developed model was capable of describing phenomena during composite preparation. Morphology analysis using scanning electron microscopy shows smooth covered fiber in compatibilized samples than that of without MNR. The morphology also showed less voids on compatibilized samples attributed to better interfacial bonding leading to tensile strength improvement.

  20. The Properties of SBR/ENR50 Blend Containing Nanoclay/Carbon Black Dual Filler System Cured by Electron Beam

    Directory of Open Access Journals (Sweden)

    Sima Ahmadi-Shooli

    2017-05-01

    Full Text Available Nanocomposites based on an SBR/ENR50 rubber blend with the blend ratio of 50/50 using Cloisite 15A nanoclay (5 and 10 phr and carbon black (20 phr were prepared by melt mixing process. The rubber compounds were crosslinked by electron beam irradiation process at 50 and 100 kGy doses. A reference sample containing carbon black at 35 phr was prepared using a conventional sulphur curing system. The gel content of the samples was specified using gel fraction measurement. The results showed the maximum gel content for the sample having 5 phr nanoclay and 20 phr carbon black. The dynamic mechanical properties, including the storage modulus, loss modulus, and loss factor, of the nanocomposites were evaluated using dynamic mechanical analysis (DMA tests. The results indicated that, in spite of a well dispersed nanoclay in samples containing 10 phr nanoclay and 20 phr carbon black, a minimum loss factor was observed in the sample containing 5 phr nanoclay and 20 phr carbon black at 100 kGy. On the other hand, the storage modulus of the reference sample was found to be higher than that of the sample with 5 phr nanoclay and 20 phr carbon black. The mechanical properties, including the tensile strength, stress at 100%, 200%, and 300% elongation and the percentage of elongation were measured by a tensile machine. The results showed an increase in tensile strength and the stress at different elongations for a sample with 5 phr nanoclay and 20 phr carbon black compared to the reference sample. In the corresponding SEM images of the samples having nanoclay and carbon black irradiated at 100 kGy a significantly higher surface roughness was observed.

  1. Histometría de la glándula sublingual de ratones (Mus musculus machos y hembras infectados con la cepa RAL del parásito de Chagas, Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Sérgio de Albuquerque

    2008-06-01

    Full Text Available Analizamos morfológica e histométricamente la glándula sublingual de ratones infectados con la cepa RAL del Trypanosoma cruzi, en machos y hembras. Usamos ratones albinos (Mus musculus, variedad Swiss, inoculados con 2x104 tripomastigotes sanguíneos de la cepa RAL del T. cruzi.. Sacrificamos los animales en el pico de la parasitemia (12º día y fijamos las glándulas sublinguales en ALFAC. Los cortes histológicos teñidos con HE fueron evaluados histométricamente (cariometría y estereología. La parasitemia fue más elevada en las hembras. Histopatológicamente, los "ácinos" (acini de los animales infectados eran menores, con escasa secreción, y conductos estriados menores. Los núcleos de las "medialunas" eran menores y había nidos de amastigotes en el citoplasma. Cariométricamente, los núcleos de los ácinos, medialunas y conductos estriados eran menores en los ratones infectados. Estereológicamente, los volúmenes relativos ocupados por ácinos y conductos estriados fueron menores e, inversamente, fue mayor el volumen para el tejido conjuntivo de los machos infectados. Las densidades de superficie de ácinos y conductos fueron mayores, y el diámetro y el espesor de la pared menores, en este grupo. Por otro lado, la densidad de ácinos fue menor, y las de los conductos estriados y tejido conjuntivo, fueron mayores en las hembras infectadas. Las densidades de superficie de ácinos y conductos estriados fueron mayores, mientras que el diámetro y espesor de la pared de los ácinos fueron menores (y las de los conductos estriados mayores, en este grupo. La cepa RAL del T. cruzi causó un cuadro general de atrofia general en la glándula sublingual, con numerosos nidos de parásitos.Histometry of the sublingual gland in male and female mice (Mus musculus infected with the RAL strain of the Chagas parasite, Trypanosoma cruzi. The aim of this work was to analyze histologically and histometrically the sublingual gland of mice infected

  2. Bridging the digital divide: mobile access to personal health records among patients with diabetes.

    Science.gov (United States)

    Graetz, Ilana; Huang, Jie; Brand, Richard J; Hsu, John; Yamin, Cyrus K; Reed, Mary E

    2018-01-01

    Some patients lack regular computer access and experience a digital divide that causes them to miss internet-based health innovations. The diffusion of smartphones has increased internet access across the socioeconomic spectrum, and increasing the channels through which patients can access their personal health records (PHRs) could help bridge the divide in PHR use. We examined PHR use through a computer-based Web browser or mobile device. Cross-sectional historical cohort analysis. Among adult patients in the diabetes registry of an integrated healthcare delivery system, we studied the devices used to access their PHR during 2016. Among 267,208 patients with diabetes, 68.1% used the PHR in 2016; 60.6% of all log-ins were via computer and 39.4% were via mobile device. Overall, 63.9% used it from both a computer and mobile device, 29.6% used only a computer, and 6.5% used only a mobile device. After adjustment, patients who were black, Hispanic, or Asian; lived in lower socioeconomic status (SES) neighborhoods; or had lower engagement were all significantly more likely to use the PHR only from a mobile device (P digital divide in computer use, disproportionately reaching racial/ethnic minorities and lower SES patients. Nonetheless, even with a mobile-optimized and app-accessible PHR, differences in PHR use by race/ethnicity and SES remain. Continued efforts are needed to increase equitable access to PHRs among patients with chronic conditions.

  3. Morphology, thermal and mechanical properties of PVC/MMT nanocomposites prepared by solution blending and solution blending + melt compounding

    DEFF Research Database (Denmark)

    Madaleno, Liliana Andreia Oliveira; Schjødt-Thomsen, Jan; Pinto, José Cruz

    2010-01-01

    Two types of montmorillonite (MMT), natural sodium montmorillonite (Na-MMT) and organically modified montmorillonite (OMMT), in different amounts of 1, 2, 5, 10 and 25 phr (parts per hundred resin), were dispersed in rigid poly (vinyl chloride) by two different methods solution blending...... and solution blending + melt compounding The effects on morphology, thermal and mechanical properties of the PVC/MMT nanocomposites were studied by varying the amount of Na-MMT and OMMT in both methods SEM and XRD analysis revealed that possible intercalated and exfoliated structures were obtained in all...... prepared by solution blending + melt compounding method Experimental values for 1 and 2 phr are larger than the calculated values which directly suggest that the MMT particles are exfoliated (C) 2010 Elsevier Ltd All rights reserved...

  4. NCBI nr-aa BLAST: CBRC-RNOR-04-0256 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0256 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  5. NCBI nr-aa BLAST: CBRC-RNOR-04-0238 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0238 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  6. NCBI nr-aa BLAST: CBRC-MMUS-06-0122 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0122 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  7. NCBI nr-aa BLAST: CBRC-OCUN-01-1141 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-1141 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  8. NCBI nr-aa BLAST: CBRC-MMUS-06-0135 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0135 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  9. NCBI nr-aa BLAST: CBRC-RNOR-04-0259 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0259 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9EQ46|VN1B7_MOUSE Vomeronas...al type-1 receptor B7 (Vomeronasal type-1 receptor A11) gb|AAG42087.1|AF291493_1 vomeronasa...l receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A11 [Mus musculus] gb|AAI41627.1| Vomeronasa...l 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasal

  10. Dicty_cDB: VHD239 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available lignments: (bits) Value BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 112 3e-23 BC13...8482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 112 4e-23 (Q9NZJ4) RecName: Full=Sacsin; &...AL157766_4( AL157766 |pid:none) 112 4e-23 (Q9JLC8) RecName: Full=Sacsin; 112 4e-2

  11. Dicty_cDB: Contig-U10276-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1_1( AB019491 |pid:none) Homo sapiens IDN4-GGTR8 mRNA, part... 39 0.52 ( Q92738 ) RecName: Full=USP6 N-terminal-li...em... 40 0.18 BC051184_1( BC051184 |pid:none) Mus musculus USP6 N-terminal like,... 39 0.31 (Q66K14) RecName...: Full=TBC1 domain family member 9B; &AB449893_... 39 0.31 (Q80XC3) RecName: Full=USP6 N-terminal-like prote...7489 ) 1095460264250 Global-Ocean-Sampling_GS-31-01-01-1... 44 0.91 2 ( EA427602 ) Sequence 77 from patent U...ns chromosome 12 clone ABC7-42389800N19... 46 3.3 1 ( ER597226 ) 1093016226857 Global-Ocean-Sampling_GS-3

  12. Respiratory Highlights, 2016 - 2017 Influenza Season (2 October 2016 - 31 September 2017)

    Science.gov (United States)

    2018-02-21

    Medicine Public Health and Preventive Medicine Dept/PHR 2510 Fifth St., Bldg. 840 Wright-Patterson AFB, OH 45433-7913 8. PERFORMING ORGANIZATION...Surveillance Program Cleared, 88PA, Case # 2018-1051, 5 Mar 2018. 1 USAF School of Aerospace Medicine & Defense Health Agency...influenza from an infected child with a history of swine contact at an agricultural event in Texas. The HA gene from select influenza positives was

  13. NCBI nr-aa BLAST: CBRC-STRI-01-1973 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-1973 ref|NP_444458.1| vomeronasal 1 receptor, B7 [Mus musculus] sp|Q9E...Q46|VN1B7_MOUSE RecName: Full=Vomeronasal type-1 receptor B7; AltName: Full=Vomeronasal type-1 receptor A11 ...gb|AAG42087.1|AF291493_1 vomeronasal receptor V1RB7 [Mus musculus] dbj|BAB79217.1| vomeronasal receptor 1 A1...1 [Mus musculus] gb|AAI41627.1| Vomeronasal 1 receptor, B7 [synthetic construct] gb|AAI40251.1| Vomeronasa

  14. Early gene expression divergence between allopatric populations of the house mouse (Mus musculus domesticus).

    Science.gov (United States)

    Bryk, Jarosław; Somel, Mehmet; Lorenc, Anna; Teschke, Meike

    2013-03-01

    Divergence of gene expression is known to contribute to the differentiation and separation of populations and species, although the dynamics of this process in early stages of population divergence remains unclear. We analyzed gene expression differences in three organs (brain, liver, and testis) between two natural populations of Mus musculus domesticus that have been separated for at most 3000 years. We used two different microarray platforms to corroborate the results at a large scale and identified hundreds of genes with significant expression differences between the populations. We find that although the three tissues have similar number of differentially expressed genes, brain and liver have more tissue-specific genes than testis. Most genes show changes in a single tissue only, even when expressed in all tissues, supporting the notion that tissue-specific enhancers act as separable targets of evolution. In terms of functional categories, in brain and to a smaller extent in liver, we find transcription factors and their targets to be particularly variable between populations, similar to previous findings in primates. Testis, however, has a different set of differently expressed genes, both with respect to functional categories and overall correlation with the other tissues, the latter indicating that gene expression divergence of potential importance might be present in other datasets where no differences in fraction of differentially expressed genes were reported. Our results show that a significant amount of gene expression divergence quickly accumulates between allopatric populations.

  15. NCBI nr-aa BLAST: CBRC-STRI-01-1277 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-1277 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9E...Q52|VN1A3_MOUSE RecName: Full=Vomeronasal type-1 receptor A3; AltName: Full=Vomeronasal type-1 receptor A6 g...b|AAG42076.1|AF291482_1 vomeronasal receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 ...[Mus musculus] gb|AAI41612.1| Vomeronasal 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasa

  16. NCBI nr-aa BLAST: CBRC-STRI-01-2495 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2495 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9E...Q52|VN1A3_MOUSE RecName: Full=Vomeronasal type-1 receptor A3; AltName: Full=Vomeronasal type-1 receptor A6 g...b|AAG42076.1|AF291482_1 vomeronasal receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 ...[Mus musculus] gb|AAI41612.1| Vomeronasal 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasa

  17. NCBI nr-aa BLAST: CBRC-STRI-01-2240 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2240 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9E...Q52|VN1A3_MOUSE RecName: Full=Vomeronasal type-1 receptor A3; AltName: Full=Vomeronasal type-1 receptor A6 g...b|AAG42076.1|AF291482_1 vomeronasal receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 ...[Mus musculus] gb|AAI41612.1| Vomeronasal 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasa

  18. NCBI nr-aa BLAST: CBRC-STRI-01-2627 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2627 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9E...Q52|VN1A3_MOUSE RecName: Full=Vomeronasal type-1 receptor A3; AltName: Full=Vomeronasal type-1 receptor A6 g...b|AAG42076.1|AF291482_1 vomeronasal receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 ...[Mus musculus] gb|AAI41612.1| Vomeronasal 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasa

  19. Study on irradiation condition in radiation vulcanization of natural rubber latex

    International Nuclear Information System (INIS)

    Zhu Nankang; Wang Chunlei; Makuuchi, K.; Yoshii, F.

    1996-01-01

    The effect of gamma rays irradiation dose rates on RVNRL preparation was studied using Malaysian latex added with O.2 phr of KOH and 5 phr n-BA. The results showed, to ensure the tensile strength of the latex film meet the requirement, when applying vulcanisation doses, Dv of 20 kGy 20 and 15 kGy, irradiation dose rates should not be greater than 0. 49 kGy/hr and 1. 6 kGy/hr respectively. Its was found that within the storage time of 20 days there was no change in the physical properties of the latex films

  20. Identification of an active ID-like group of SINEs in the mouse.

    Science.gov (United States)

    Kass, David H; Jamison, Nicole

    2007-09-01

    The mouse genome consists of five known families of SINEs: B1, B2, B4/RSINE, ID, and MIR. Using RT-PCR we identified a germ-line transcript that demonstrates 92.7% sequence identity to ID (excluding primer sequence), yet a BLAST search identified numerous matches of 100% sequence identity. We analyzed four of these elements for their presence in orthologous genes in strains and subspecies of Mus musculus as well as other species of Mus using a PCR-based assay. All four analyzed elements were identified either only in M. musculus or exclusively in both M. musculus and M. domesticus, indicative of recent integrations. In conjunction with the identification of transcripts, we present an active ID-like group of elements that is not derived from the proposed BC1 master gene of ID elements. A BLAST of the rat genome indicated that these elements were not in the rat. Therefore, this family of SINEs has recently evolved, and since it has thus far been observed mainly in M. musculus, we refer to this family as MMIDL.

  1. Spermatogenic structure and fertility of Mus musculus after exposure of mangosteen (Garcinia mangostana L) pericarp extract

    Science.gov (United States)

    Hayati, Alfiah; Agustin, Melia Eka; Rokhimaningrum, Farida Ayu; Adro'i, Hasan; Darmanto, Win

    2016-03-01

    This study aimed to determine the effect of mangosteen (Garcinia mangostana L.) pericarp extract on spermatogenics number, seminiferous tubules sized, profile protein of epididymal and testicular sperm, and fertility of mice (Mus musculus). Fourty two male mice strain BALB/C was divided equally into 7 groups. The control group was given 0.05 ml of 0.05% CMC solution. Three group were given mangosteen pericarp extract at various doses (75, 100 and 150 mg/kg body weight, respectively) for 7 days, while the other three groups were given the same extract dose for 35 days. Parameters evaluated on histological of spermatogonia, spermatocytes, round spermatids, seminiferous tubule diameter, and thickness of germinal epithelium, analysis of testicular and epidydimal protein profile with SDS-Page, and than fertility test on female mice. The results showed that mangosteen pericarp extract at 75 and 100 mg/kg dose for 7 days had no effect on spermatogenics number and seminiferous tubule sizes, but the treatment dose of 150 mg/kg for 7 days and all treatment (doses of 75, 100, and 150 mg/kg) for 35 days led to significant decrease on the number of spermatogenics and seminiferous tubule sizes; effect on protein profiles testicular and epididymal sperm; and lower fertilization.

  2. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

    Energy Technology Data Exchange (ETDEWEB)

    Li, Chongjie [Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061 (China); Ma, Li [Key Laboratory of Biofuels, and Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101 (China); Mou, Shanli [Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao (China); Wang, Yibin, E-mail: wangyibin@fio.org.cn [Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061 (China); Zheng, Zhou; Liu, Fangming; Qi, Xiaoqing; An, Meiling; Chen, Hao [Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061 (China); Miao, Jinlai, E-mail: miaojinlai@163.com [Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061 (China); State Key Laboratory of Biological Fermentation Engineering of Beer (In Preparation), Qingdao (China)

    2015-03-15

    Highlights: • Chlamydomonas sp. ICE-L photolyase gene PHR2 is first cloned and expressed in E. coli. • PHR2 complemented E. coli could efficiently survival from UV radiation. • Expressed PHR2 photolyase has distinct photo-reactivation activity in vitro. - Abstract: Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation. A putative photolyase was identified in the Chlamydomonas sp. ICE-L transcriptome. The complete cDNA sequence was obtained by RACE-PCR. This PHR encoding includes a polypeptide of 579 amino acids with clear photolyase signatures belonging to class II CPD-photolyases, sharing a high degree of homology with Chlamydomonas reinhardtii (68%). Real-time PCR was performed to investigate the potential DNA damage and responses following UVB exposure. CPD photolyase mRNA expression level increased over 50-fold in response to UVB radiation for 6 h. Using photolyase complementation assay, we demonstrated that DNA photolyase increased photo-repair more than 116-fold in Escherichia coli strain SY2 under 100 μw/cm{sup 2} UVB radiation. To determine whether photolyase is active in vitro, CPD photolyase was over-expressed. It was shown that pyrimidine dimers were split by the action of PHR2. This study reports the unique structure and high activity of the enzyme. These findings are relevant for further understanding of molecular mechanisms of photo-reactivation, and will accelerate the utilization of photolyase in the medical field.

  3. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

    International Nuclear Information System (INIS)

    Li, Chongjie; Ma, Li; Mou, Shanli; Wang, Yibin; Zheng, Zhou; Liu, Fangming; Qi, Xiaoqing; An, Meiling; Chen, Hao; Miao, Jinlai

    2015-01-01

    Highlights: • Chlamydomonas sp. ICE-L photolyase gene PHR2 is first cloned and expressed in E. coli. • PHR2 complemented E. coli could efficiently survival from UV radiation. • Expressed PHR2 photolyase has distinct photo-reactivation activity in vitro. - Abstract: Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation. A putative photolyase was identified in the Chlamydomonas sp. ICE-L transcriptome. The complete cDNA sequence was obtained by RACE-PCR. This PHR encoding includes a polypeptide of 579 amino acids with clear photolyase signatures belonging to class II CPD-photolyases, sharing a high degree of homology with Chlamydomonas reinhardtii (68%). Real-time PCR was performed to investigate the potential DNA damage and responses following UVB exposure. CPD photolyase mRNA expression level increased over 50-fold in response to UVB radiation for 6 h. Using photolyase complementation assay, we demonstrated that DNA photolyase increased photo-repair more than 116-fold in Escherichia coli strain SY2 under 100 μw/cm 2 UVB radiation. To determine whether photolyase is active in vitro, CPD photolyase was over-expressed. It was shown that pyrimidine dimers were split by the action of PHR2. This study reports the unique structure and high activity of the enzyme. These findings are relevant for further understanding of molecular mechanisms of photo-reactivation, and will accelerate the utilization of photolyase in the medical field

  4. Human-centered design of a personal health record system for metabolic syndrome management based on the ISO 9241-210:2010 standard.

    Science.gov (United States)

    Farinango, Charic D; Benavides, Juan S; Cerón, Jesús D; López, Diego M; Álvarez, Rosa E

    2018-01-01

    Previous studies have demonstrated the effectiveness of information and communication technologies to support healthy lifestyle interventions. In particular, personal health record systems (PHR-Ss) empower self-care, essential to support lifestyle changes. Approaches such as the user-centered design (UCD), which is already a standard within the software industry (ISO 9241-210:2010), provide specifications and guidelines to guarantee user acceptance and quality of eHealth systems. However, no single PHR-S for metabolic syndrome (MS) developed following the recommendations of the ISO 9241-210:2010 specification has been found in the literature. The aim of this study was to describe the development of a PHR-S for the management of MS according to the principles and recommendations of the ISO 9241-210 standard. The proposed PHR-S was developed using a formal software development process which, in addition to the traditional activities of any software process, included the principles and recommendations of the ISO 9241-210 standard. To gather user information, a survey sample of 1,187 individuals, eight interviews, and a focus group with seven people were performed. Throughout five iterations, three prototypes were built. Potential users of each system evaluated each prototype. The quality attributes of efficiency, effectiveness, and user satisfaction were assessed using metrics defined in the ISO/IEC 25022 standard. The following results were obtained: 1) a technology profile from 1,187 individuals at risk for MS from the city of Popayan, Colombia, identifying that 75.2% of the people use the Internet and 51% had a smartphone; 2) a PHR-S to manage MS developed (the PHR-S has the following five main functionalities: record the five MS risk factors, share these measures with health care professionals, and three educational modules on nutrition, stress management, and a physical activity); and 3) usability tests on each prototype obtaining the following results: 100

  5. Dicty_cDB: Contig-U01585-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available mRNA, clone: ARN01A01NGRM0003_F0... 54 0.010 1 ( GE817509 ) EST_scau_evk_969928 scauevk mixed_tissue Sebaste...s... 54 0.010 1 ( GE798818 ) EST_scau_evk_889236 scauevk mixed_tissue Sebastes... 54 0.010 1 ( FK742846 ) av...Mus musculus NOD-derived CD11c +ve dendritic cell... 52 0.038 1 ( GE798817 ) EST_scau_evk_887700 scauevk mixed_tissue Sebaste

  6. Thermal properties of silica-filled high density polyethylene composites compatibilized with glut palmitate

    Science.gov (United States)

    Samsudin, Dalina; Ismail, Hanafi; Othman, Nadras; Hamid, Zuratul Ain Abdul

    2017-07-01

    A study of thermal properties resulting from the utilization of Glut Palmitate (GP) on the silica filled high density polyethylene (HDPE) composites was carried out. The composites with the incorporation of GP at 0.5, 1.0, 2.0 and 3.0 phr were prepared by using an internal mixer at the temperature 180 °C and the rotor speed of 50 rpm. The thermal behaviours of the composites were then investigated using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). It was found that the crystallinity and the thermal stability of the composites increased with the incorporation of GP. The highest crystallinity contents and decomposition temperatures were observed at the 1 phr GP loading.

  7. Multilayer graphene rubber nanocomposites

    Science.gov (United States)

    Schartel, Bernhard; Frasca, Daniele; Schulze, Dietmar; Wachtendorf, Volker; Krafft, Bernd; Morys, Michael; Böhning, Martin; Rybak, Thomas

    2016-05-01

    Multilayer Graphene (MLG), a nanoparticle with a specific surface of BET = 250 m2/g and thus made of only approximately 10 graphene sheets, is proposed as a nanofiller for rubbers. When homogenously dispersed, it works at low loadings enabling the replacement of carbon black (CB), increase in efficiency, or reduction in filler concentration. Actually the appropriate preparation yielded nanocomposites in which just 3 phr are sufficient to significantly improve the rheological, curing and mechanical properties of different rubbers, as shown for Chlorine-Isobutylene-Isoprene Rubber (CIIR), Nitrile-Butadiene Rubber (NBR), Natural Rubber (NR), and Styrene-Butadiene Rubber (SBR). A mere 3 phr of MLG tripled the Young's modulus of CIIR, an effect equivalent to 20 phr of carbon black. Similar equivalents are observed for MLG/CB mixtures. MLG reduces gas permeability, increases thermal and electrical conductivities, and retards fire behavior. The later shown by the reduction in heat release rate in the cone calorimeter. The higher the nanofiller concentration is (3 phr, 5 phr, and 10 phr was investigated), the greater the improvement in the properties of the nanocomposites. Moreover, the MLG nanocomposites improve stability of mechanical properties against weathering. An increase in UV-absorption as well as a pronounced radical scavenging are proposed and were proved experimentally. To sum up, MLG is interesting as a multifunctional nanofiller and seems to be quite ready for rubber development.

  8. Efeitos da música clássica como elemento de enriquecimento ambiental em Mus musculus em cativeiro (Rodentia: Muridae

    Directory of Open Access Journals (Sweden)

    José Geraldo Pereira da Cruz

    2010-04-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2010v23n2p191 No ambiente selvagem, os animais são submetidos a mudanças constantes nos estímulos sensoriais. Entretanto, o ambiente de cativeiro é muito mais pobre em termos de estímulos fornecidos aos animais alojados no seu interior. Numa tentativa de remediar esta situação e promover um melhor bem estar, camundongos (Mus musculus foram expostos a duas condições: sem estímulos auditivos e com música clássica. Em todos os experimentos foi utilizada uma bateria de testes comportamentais. Os resultados demonstram uma diminuição significativa na imobilidade no nado forçado, aumento de entradas nos braços fechados do labirinto em cruz elevado e diminuição na imobilidade no campo aberto, nos animais que haviam sido pré-expostos anteriormente a 24h de música e sugerem que a música clássica causas mudanças na atividade motora em camundongos. Este estudo leva a conclusão que o enriquecimento do ambiente causa profundos efeitos sobre o comportamento de camundongos nos testes comportamentais e a música clássica é um método relativamente simples de contribuir para o bem estar de camundongos em cativeiro, mas pode comprometer resultados de experimentos como o nado forçado.

  9. NCBI nr-aa BLAST: CBRC-OCUN-01-0928 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0928 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9EQ52|VN1A3_MOUSE Vomeronas...al type-1 receptor A3 (Vomeronasal type-1 receptor A6) gb|AAG42076.1|AF291482_1 vomeronasa...l receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 [Mus musculus] gb|AAI41612.1| Vomeronasa...l 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasal 1 receptor, A3 [synthetic construct] NP_444448.1 1e-81 52% ...

  10. NCBI nr-aa BLAST: CBRC-CPOR-01-0933 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-0933 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9EQ52|VN1A3_MOUSE Vomeronas...al type-1 receptor A3 (Vomeronasal type-1 receptor A6) gb|AAG42076.1|AF291482_1 vomeronasa...l receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 [Mus musculus] gb|AAI41612.1| Vomeronasa...l 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasal 1 receptor, A3 [synthetic construct] NP_444448.1 7e-78 52% ...

  11. NCBI nr-aa BLAST: CBRC-MMUS-06-0143 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0143 ref|NP_444448.1| vomeronasal 1 receptor, A3 [Mus musculus] sp|Q9EQ52|VN1A3_MOUSE Vomeronas...al type-1 receptor A3 (Vomeronasal type-1 receptor A6) gb|AAG42076.1|AF291482_1 vomeronasa...l receptor V1RA3 [Mus musculus] dbj|BAB79212.1| vomeronasal receptor 1 A6 [Mus musculus] gb|AAI41612.1| Vomeronasa...l 1 receptor, A3 [synthetic construct] gb|AAI40226.1| Vomeronasal 1 receptor, A3 [synthetic construct] NP_444448.1 4e-85 55% ...

  12. Synaptic vesicles isolated from the electric organ of Torpedo californica and from the central nervous system of Mus musculus contain small ribonucleic acids (sRNAs

    Directory of Open Access Journals (Sweden)

    Huinan Li

    2017-06-01

    Full Text Available Synaptic vesicles (SVs are presynaptic organelles that load and release small molecule neurotransmitters at chemical synapses. In addition to classic neurotransmitters, we have demonstrated that SVs isolated from the Peripheral Nervous Systems (PNS of the electric organ of Torpedo californica, a model cholinergic synapse, and SVs isolated from the Central Nervous System (CNS of Mus musculus (mouse contain small ribonucleic acids (sRNAs; ≤50 nucleotides (Scientific Reports, 5:1–14(14918 Li et al. (2015 [1]. Our previous publication provided the five most abundant sequences associated with the T. californica SVs, and the ten most abundant sequences associated with the mouse SVs, representing 59% and 39% of the total sRNA reads sequenced, respectively. We provide here a full repository of the SV sRNAs sequenced from T. californica and the mouse deposited in the NCBI as biosamples. Three data studies are included: SVs isolated from the electric organ of T. californica using standard techniques, SVs isolated from the electric organ of T. californica using standard techniques with an additional affinity purification step, and finally, SVs isolated from the CNS of mouse. The three biosamples are available at https://www.ncbi.nlm.nih.gov/biosample/ SRS1523467, SRS1523466, and SRS1523472 respectively.

  13. Development and evaluation of an internet and personal health record training program for low-income patients with HIV or hepatitis C.

    Science.gov (United States)

    McInnes, D Keith; Solomon, Jeffrey L; Shimada, Stephanie L; Petrakis, Beth A; Bokhour, Barbara G; Asch, Steven M; Nazi, Kim M; Houston, Thomas K; Gifford, Allen L

    2013-03-01

    Vulnerable populations face difficulties accessing and using the internet and personal health record (PHR) systems for health-related purposes. Populations disconnected from the internet also tend to be disconnected from health care services. To develop and evaluate an intervention to increase skills in health-related internet and PHR use for vulnerable populations with limited computer and internet experience. Preevaluation and postevaluation using quantitative surveys, semistructured interviews, focus groups, and ethnographic observation. Fourteen low-income Veterans receiving care at Veterans Affairs medical centers for human immunodeficiency virus or hepatitis C. Internet and PHR use, self-efficacy, patient activation, disease knowledge, predictors of medication adherence. At follow-up one (FU1), mean number of internet for health features used increased from 1.57 to 4.07 (Pinternet and PHRs and their contribution to increased engagement in care. Training cost per participant was $287. Group training of vulnerable patients represents a cost-effective method to increase internet and PHR skills, and improve patient confidence in finding health-related information, making online health-related transactions, and interacting with health care providers.

  14. Maternal-foetal genomic conflict and speciation: no evidence for hybrid placental dysplasia in crosses between two house mouse subspecies

    Czech Academy of Sciences Publication Activity Database

    Kropáčková, L.; Piálek, Jaroslav; Gergelits, Václav; Forejt, Jiří; Reifová, R.

    2015-01-01

    Roč. 28, č. 3 (2015), s. 688-698 ISSN 1010-061X R&D Projects: GA ČR GA13-08078S Institutional support: RVO:68081766 ; RVO:68378050 Keywords : hybrid placental dysplasia * genomic conflicts * speciation * X chromosome * house mouse * Mus musculus musculus * Mus musculus domesticus Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.747, year: 2015

  15. Young adults on the perceived benefits and expected use of personal health records: a qualitative descriptive study.

    Science.gov (United States)

    Nguyen, Quynh; Bartlett, Gillian; Rodriguez, Charo; Tellier, Pierre-Paul

    2016-04-18

    Personal health records (PHRs) are tools that allow individuals to access, share and manage their health information online. Despite apparent interest, adoption rates remain low. There is a gap in our understanding as to what different populations of users, in particular young adults, might want from such a tool. To describe and interpret the views and expectations of young healthy adults about using an online PHR. A qualitative descriptive study was carried out. Four focus groups were conducted with a total of 29 participants (18-34 years old) from a community setting in Montreal, Canada. Interviews were transcribed and analyzed with inductivethematic analysis. With respect to how young adults viewed PHRs, three broad themes were identified: perceived advantages to using a PHR, future PHR users and concerns about PHRs. Three other overarching themes emerged from data analysis in terms of what participants expected from using a PHR: the use of the PHR for preventative health, PHR support to take more control over their health and strategies to make the PHR worthwhile. A conceptual framework of factors influencing expectations of PHR use in this population is proposed. While young adults view the PHR as beneficial, this is not enough for them to be motivated to actually use a PHR. To foster use, the PHRs need to be perceived as a health prevention tool that helps users to increase control over theirhealth status. More research is needed to understand the expectations and anticipated use of different populations in designing a person-centered tool;the proposedframework provides theoretical basis in this regard.

  16. Development of an efficient process for radiation vulcanization of natural rubber latex using hydroperoxide with sensitizer

    International Nuclear Information System (INIS)

    Siri-upathum, C.; Sonsuk, M.

    1996-01-01

    An attempt was made to reduce irradiation dose for radiation vulcanization of natural rubber latex. A promising method was to partially crosslink the latex by radiation vulcanization using n-butyl acrylate (n-BA) as sensitizer and t-butyl hydroperoxide (BHPO) as a co-sensitizer followed by redox vulcanization using residual BHPO as an oxidant and either fructose or tetra ethylene penta mine as reducing agents. It was found that the irradiation dose was reduced to 4 kGy with 5 phr n-BA as sensitizer and 0.1 phr BHPO as co-sensitizer. Successive crosslinking to full vulcanization was done by redox vulcanization using either 4 phr fructose at 60 degree C for 3 hours of 0.4 phr tetra-ethylene penta mine at room temperature for 1 hour. The rubber films obtained had tensile strength of about 25 MPa, modulus 300% of 0.9 MPa and crosslink density of about 1.5 x 10 19 crosslink/cm 3 . It was noted that the rubber film from the co-vulcanization was the average value of the values obtained by radiation vulcanization and redox vulcanization

  17. Improving stroke patients' care: a patient held record is not enough

    Directory of Open Access Journals (Sweden)

    Lampe Fiona

    2001-03-01

    Full Text Available Abstract Background Stroke patients' care in hospital tends to be poorly organised, with poor communication and a lack of information being frequent sources of complaint. The purpose of this study was to evaluate whether a patient-held record (PHR would result in greater patient satisfaction and better care planning for stroke patients. Methods A time series control (6 months - intervention (8 months - control (6 months was used among London teaching hospital general medical and geriatric medicine inpatient wards. All stroke patients admitted to the wards during the intervention phase received a PHR and were instructed in its use. Demographic, stroke severity, social factors and outcomes were collected from all stroke patients during all phases of the study. Results Of 252 stroke patients aged 46 to 98 years entered into the study, by six months after admission 118 (46.8% had died. PHR and control group patients were well matched in terms of socio-demographic characteristics and pre-stroke ability. At six months after admission, 119 (97% patients responded to the questionnaire. Just over half (56%, 13 of intervention group patients recalled receiving a PHR. Of those patients, 59% reported reading the PHR, 27% had lost their PHR, and two-thirds said they had difficulties encouraging staff to write in the PHR. Half felt that possession of the PHR was more trouble than it was worth. PHR group patients were more satisfied with the recovery they had made (79% vs. 59%, p=0.04, but felt less able to talk to staff about their problems (61% vs. 82%, p=0.02. PHR group patients reported receiving fewer explanations about their condition (18% vs. 33%, p=0.12 and treatment (26% vs. 45%, p=0.07, and were more afraid of asking doctors questions (21% vs. 4%, p=0.01 than controls. PHR group patients were no better prepared for hospital discharge than control group patients, and both groups were ill-informed about services and benefits that might have helped

  18. Experiment list: SRX186172 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 1=YY1 || chip antibody manufacturer 1=Abcam || chip antibody 2=YY1 || chip antibody manufacturer 2=Santa Cru...ip-Seq; Mus musculus; ChIP-Seq source_name=Rag1 -/- pro-B cells || chip antibody

  19. Curcumin Is Efective In Improving Folliculogenesis Profile And Oo-cytes Quality In Vitro In Ectopic Endometriosis Mouse (Mus muscu-lus Models

    Directory of Open Access Journals (Sweden)

    Aulia Firmawati

    2018-02-01

    Full Text Available Endometriosis is the presence of ectopic endometrial tissue, such as that outside the uter-ine cavity, which can cause chronic inflammatory reactions. Curcumin is one of traditional herbal med-icines that is widely used. Some experiments have managed to find a mechanism to treat an ectopic endometriosis through the mechanism of suppression of several cytokines such as TNF-alpha, NF-kB and COX-2, this study was an experimental laboratory study, with five treatments and six replications, using female mice (Mus musculus that had reached puberty. The study consisted of three phases: the first phase was the microscopic examination of in vitro oocytes quality, and the second was histopatho-logical examination of folliculogenesis profile using Hematoxillin eosin staining, the results of this study showed significant difference between positive control group (P0 with treatment and negative control (KN groups in folliculogenesis profile (p <0.05. Examination of in vitro oocytes quality showed sig-nificant difference between positive control group with treatment group and negative control group (p <0.05. Curcumin has several roles in cytokines modulation in ectopic endometriosis mouse models. Curcumin may reduce the occurrence of apoptosis of granulosa cells so that it may directly improve oocyte quality and folliculogenesis profile, in conclusion, curcumin effectively overcomes fertility prob-lems through the mechanism of cytokines modulation that plays a role in some cases of endometriosis such as: TNF-alpha, NF-kB and COX-2. Curcumin may improve folliculogenesis profile and in vitro oocytes quality.

  20. Pembuatan lem sintetik dan aplikasinya pada alas kaki

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2008-12-01

    Full Text Available Footwear can be categorized as one of labour intensive and very superior export commodities, consequently the product quality must be maintained and be continually improved. The quality parameter of footwear is determined by the bonding strength between the uppers and the soles, it means that it depends on the adhesive applied. Soling system is widely applied in small and medium industries are cemented system used synthetic adhesive. Now, the adhesive was applied in the manufacturing of foot wear supported by binding of the soles and the uppers is easy to peel off because of the poor bonding strength. The aim of the research was to investigate a suitable synthetic adhesive formula to used in footwear manufacturing. In preparing of the adhesive formula, the effect of phenolic resin addition as tackifier were varies i.e 35, 45 and 55 phr using chloroprene rubber as raw material were observed. Synthetic adhesive compound was produce by two roll mill after that compound were dissolved in toluene with ratio 1 : 3. The prepared adhesive then was applied to manufacture footwear using cool press system. The soles used in the experiment were made of rubber and plastic material whereas the uppers made of either leather or artificial leather. The test result and data analysis showed that adhesive performed for the highest bonding strength as much as 1000 g/cm and decrease to lowest bonding strength after soaking in to water for 500 seconds one percent was obtained by the following formula that were : chloroprene rubber 100 phr, butylated hydroxyl toluene 2 phr, magnesium oxide 4 phr, zinc oxide 5 phr and phenolic resin 45 phr. The prepared formula performed a better bonding strength when it was compared with chloroperene rubber adhesive in market. The application of this prepared formula in making ladies shoes performed good bonding strength and accordance with the requierments of SNI 12-2942-1992 about Leather Pantopel Style Cemented System Ladies Shoes.

  1. ORF Alignment: NC_003103 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available s] ref|NP_766317.1| caspase recruitment domain 4 ... [Mus musculus] gb|AAH42670.1| Caspase recruitment... domain ... 4 [Mus musculus] sp|Q8BHB0|CARD4_MOUSE Caspase ... recruitment domain protein 4 d

  2. How to Create a Personal Health Record

    Science.gov (United States)

    ... for managing your health Surgeon General's Family Health History Initiative More PHR Success Stories I learned that a PHR saves time, energy, and money. And it saved my life! A woman's Facebook PHR saved her life In The Blogs Get ...

  3. Mus musculus bone fluoride concentration as a useful biomarker for risk assessment of skeletal fluorosis in volcanic areas.

    Science.gov (United States)

    Linhares, Diana; Camarinho, Ricardo; Garcia, Patrícia Ventura; Rodrigues, Armindo Dos Santos

    2018-08-01

    Fluoride is often found in elevated concentrations in volcanic areas due to the release of magmatic fluorine as hydrogen fluorine through volcanic degassing. The exposure to high levels of fluoride can affect the processes of bone formation and resorption causing skeletal fluorosis, a pathology that can easily be mistaken for other skeletal diseases. In this study, we aimed to determine if fluoride concentration in the femoral bone of wild populations of the house mouse (Mus musculus) is a good biomarker of exposure to active volcanic environments naturally enriched in fluoride, allowing their use in biomonitoring programs. The fluoride concentration of the whole femoral bone of 9 mice from Furnas (5 males and 4 females) and 33 mice from Rabo de Peixe (16 males and 17 females) was measured by the potentiometric method with a fluoride ion selective electrode. Fluoride in bones was significantly higher in the mice from Furnas when compared with the mice from Rabo de Peixe (616.5 ± 129.3 μg F/g vs. 253.8 ± 10.5 μg F/g). Accumulation rates were also significantly higher in the mice collected in Furnas when compared with Rabo de Peixe individuals (3.84 ± 0.52 μg F/day vs. 1.22 ± 0.06 μg F/day). The results demonstrate a significant association between exposure to fluoride in the active volcanic environment and fluoride content in bone, revealing that bone fluoride concentration is a suitable biomarker of chronic environmental exposure to fluoride. Copyright © 2018 Elsevier Ltd. All rights reserved.

  4. AcEST: DK962347 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ... 33 1.1 sp|Q7TSF1|DSG1B_MOUSE Desmoglein-1-beta OS=Mus musculus GN=Dsg1b... 31... 30 7.4 sp|Q61495|DSG1A_MOUSE Desmoglein-1-alpha OS=Mus musculus GN=Dsg1... 30 7.4 sp|Q9SIB4|WOX3_ARATH WUSC...hia co... 30 9.6 sp|Q01550|TANA_XENLA Tanabin OS=Xenopus laevis PE=2 SV=1 30 9.6 sp|Q7TSF0|DSG1C_MOUSE Desmog...VAEYVTH 428 IVG WDE LP D V E+ E+V+H Sbjct: 322 IVGGNWDEVLPVDVRVPEEIQEFVSH 347 >sp|Q7TSF1|DSG1B_MOUSE Desmogl

  5. Evidence against a photoprotective component of photoreactivation in Saccharomyces cerevisiae

    International Nuclear Information System (INIS)

    MacQuillan, A.M.; Green, G.; Perry, W.G.

    1981-01-01

    Photoreactivation-deficient (phr - ) mutants of Saccharomyces cerevisiae were shown to lack in vitro DNA-photolyase activity. A phr - mutant was then compared with a phr + strain for near-UV induced photoprotection from far-UV irradiation. Neither strain exhibited a photoprotective effect. (author)

  6. Preparation and properties of carboxylated styrene-butadiene rubber/cellulose nanocrystals composites.

    Science.gov (United States)

    Cao, Xiaodong; Xu, Chuanhui; Liu, Yuhong; Chen, Yukun

    2013-01-30

    A series of carboxylated styrene-butadiene rubber (XSBR)/cellulose nanocrystals (CNs) latex composites were successfully prepared. The vulcanization process, morphology, dynamic viscoelastic behavior, dynamic mechanical property, thermal and mechanical performance of the XSBR/CNs composites were investigated in detail. The results revealed that CNs were dispersed uniformly in the XSBR matrix and formed a strong filler-filler network. The dynamic mechanical analysis (DMA) showed that the glass transition temperature (T(g)) of XSBR matrix was shifted from 48.45 to 50.64 °C with 3 phr CNs, but decreased from 50.64 to 46.28 °C when further increasing CNs content up to 15 phr. The composites exhibited a significant enhancement in tensile strength (from 16.9 to 24.1 MPa) and tear strength (from 43.5 to 65.2 MPa) with loading CNs from 0 to 15 phr. In addition, the thermo-gravimetric analysis (TGA) showed that the temperature at 5% weight loss of the XSBR/CNs composites decreased slightly with an increase of the CNs content. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  7. Compression and shear properties of elastomeric bearing using finite element analysis

    Directory of Open Access Journals (Sweden)

    2Faculty of Science and Technology, Chiang Mai Rajabhat University, Muang, Chiang Mai, 50300 Thailand.

    2006-09-01

    Full Text Available Standard size samples of four natural rubber compounds, varying the amount of carbon black from 10 to 70 phr, were characterised under uniaxial compression and simple shear tests in order to obtain the strain energy function constants. These constants were then used as hyperelastic material constants for the Windows-based finite element package (COSMOS/M version 1.75. The investigated bearings, made with those NR compounds, had the approximate area and thickness of 50x106 mm2 and 50 mm respectively. Each compound of bearing consisted of four different values of shape factor ranging from about 0.33 to 1.70, according to the number of reinforcing plates in the bearing. Three deformation modes of compression, shear and compression-shear were predicted. Good agreement was found between twelve compression model predictions and the corresponding experimental values of bearings, containing 10, 20 and 40 phr of carbon black and each of which consisted of four different layers of reinforcing metal plates (0, 1, 2 and 3 layers. On the other hand, deviation from the predicted valve was clearly seen in the 70 phr black bearing case. The percentage difference increased with respect to the increasing number of reinforcing plates or the rising shape factor. Therefore, the improved FEA model was supplemented with an imaginary elastic glue layer between the rubber block and metal plate as glue failure compensation. The optimum value of the elastic layers modulus is 8 MPa while the thickness of the layer depends on the total thickness or total volume of rubber block. This model can predict the 70 phr carbon black bearings, having shape factor ranging from 0.5 to 2.35 for 11 cases. The FEA prediction of shear behaviour agrees well with the experimental data for all four bearing compounds and there is no effect of shape factor on shear stress. Moreover, shear stress does not depend on the compressive force applied to like bearing before shear and the FEA results

  8. Personal health records: Consumer attitudes toward privacy and security of their personal health information.

    Science.gov (United States)

    Lafky, Deborah Beranek; Horan, Thomas A

    2011-03-01

    Personal health record (PHR) systems are a subject of intense interest in the move to improve healthcare accessibility and quality. Although a number of vendors continue to put forward PHR systems, user-centered design research has lagged, and it has not been clear what features are important to prospective PHR users. Here, we report on a user-centered design study that combines qualitative and quantitative approaches to investigate several dimensions relevant to PHR design, and to look at the effect of health status on user needs. The results indicate that health status, especially disability and chronic illness, is relevant to PHR design. Further, the results provide empirical evidence about the role of privacy and security in users' attitudes toward PHR use. The exact nature of these attitudes differs from widely held perceptions about consumer values in healthcare information management. © The Author(s) 2011.

  9. Shared decision-making using personal health record technology: a scoping review at the crossroads.

    Science.gov (United States)

    Davis, Selena; Roudsari, Abdul; Raworth, Rebecca; Courtney, Karen L; MacKay, Lee

    2017-07-01

    This scoping review aims to determine the size and scope of the published literature on shared decision-making (SDM) using personal health record (PHR) technology and to map the literature in terms of system design and outcomes. Literature from Medline, Google Scholar, Cumulative Index to Nursing and Allied Health Literature, Engineering Village, and Web of Science (2005-2015) using the search terms "personal health records," "shared decision making," "patient-provider communication," "decision aid," and "decision support" was included. Articles ( n  = 38) addressed the efficacy or effectiveness of PHRs for SDM in engaging patients in self-care and decision-making or ways patients can be supported in SDM via PHR. Analysis resulted in an integrated SDM-PHR conceptual framework. An increased interest in SDM via PHR is apparent, with 55% of articles published within last 3 years. Sixty percent of the literature originates from the United States. Twenty-six articles address a particular clinical condition, with 10 focused on diabetes, and one-third offer empirical evidence of patient outcomes. The tethered and standalone PHR architectural types were most studied, while the interconnected PHR type was the focus of more recently published methodological approaches and discussion articles. The study reveals a scarcity of rigorous research on SDM via PHR. Research has focused on one or a few of the SDM elements and not on the intended complete process. Just as PHR technology designed on an interconnected architecture has the potential to facilitate SDM, integrating the SDM process into PHR technology has the potential to drive PHR value. © The Author 2017. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  10. Engineering of quorum-sensing systems for improved production of alkaline protease by Bacillus subtilis.

    NARCIS (Netherlands)

    Tjalsma, H.; Koetje, E.J.; Kiewiet, R.; Kuipers, O.P.; Kolkman, M.J.M.; Laan, J.H. van der; Daskin, R.; Ferrari, E.; Bron, S.

    2004-01-01

    AIM: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. METHODS AND RESULTS: Addition of synthetic Phr pentapeptides to the growth medium, or overproduction of pre-Phr

  11. Engineering of quorum-sensing systems for improved production of alkaline protease by Bacillus subtilis

    NARCIS (Netherlands)

    Tjalsma, H; Koetje, EJ; Kiewiet, R; Kuipers, OP; Kolkman, M; van der Laan, J; Daskin, R; Ferrari, E; Bron, S

    2004-01-01

    Aim: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. Methods and Results: Addition of synthetic Phr pentapeptides to the growth medium, or overproduction of pre-Phr

  12. Thio-urethane oligomers improve the properties of light-cured resin cements.

    Science.gov (United States)

    Bacchi, Ataís; Consani, Rafael L; Martim, Gedalias C; Pfeifer, Carmem S

    2015-05-01

    Thio-urethanes were synthesized by combining 1,6-hexanediol-diissocyante (aliphatic) with pentaerythritol tetra-3-mercaptopropionate (PETMP) or 1,3-bis(1-isocyanato-1-methylethyl)benzene (aromatic) with trimethylol-tris-3-mercaptopropionate (TMP), at 1:2 isocyanate:thiol, leaving pendant thiols. Oligomers were added at 10-30 phr to BisGMA-UDMA-TEGDMA (5:3:2, BUT). 25 wt% silanated inorganic fillers were added. Commercial cement (Relyx Veneer, 3M-ESPE) was also evaluated with 10-20 phr of aromatic oligomer. Near-IR was used to follow methacrylate conversion (DC) and rate of polymerization (Rpmax). Mechanical properties were evaluated in three-point bending (ISO 4049) for flexural strength/modulus (FS/FM, and toughness), and notched specimens (ASTM Standard E399-90) for fracture toughness (KIC). Polymerization stress (PS) was measured on the Bioman. Volumetric shrinkage (VS, %) was measured with the bonded disk technique. Results were analyzed with ANOVA/Tukey's test (α=5%). In general terms, for BUT cements, conversion and mechanical properties in flexure increased for selected groups with the addition of thio-urethane oligomers. The aromatic versions resulted in greater FS/FM than aliphatic. Fracture toughness increased by two-fold in the experimental groups (from 1.17 ± 0.36 MPam(1/2) to around 3.23 ± 0.22 MPam(1/2)). Rpmax decreased with the addition of thio-urethanes, though the vitrification point was not statistically different from the control. VS and PS decreased with both oligomers. For the commercial cement, 20 phr of oligomer increased DC, vitrification, reduced Rpmax and also significantly increased KIC, and reduced PS and FM. Thio-urethane oligomers were shown to favorably modify conventional dimethacrylate networks. Significant reductions in polymerization stress were achieved at the same time conversion and fracture toughness increased. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  13. Deriving a Set of Privacy Specific Heuristics for the Assessment of PHRs (Personal Health Records).

    Science.gov (United States)

    Furano, Riccardo F; Kushniruk, Andre; Barnett, Jeff

    2017-01-01

    With the emergence of personal health record (PHR) platforms becoming more widely available, this research focused on the development of privacy heuristics to assess PHRs regarding privacy. Existing sets of heuristics are typically not application specific and do not address patient-centric privacy as a main concern prior to undergoing PHR procurement. A set of privacy specific heuristics were developed based on a scoping review of the literature. An internet-based commercially available, vendor specific PHR application was evaluated using the derived set of privacy specific heuristics. The proposed set of privacy specific derived heuristics is explored in detail in relation to ISO 29100. The assessment of the internet-based commercially available, vendor specific PHR application indicated numerous violations. These violations were noted within the study. It is argued that the new derived privacy heuristics should be used in addition to Nielsen's well-established set of heuristics. Privacy specific heuristics could be used to assess PHR portal system-level privacy mechanisms in the procurement process of a PHR application and may prove to be a beneficial form of assessment to prevent the selection of a PHR platform with a poor privacy specific interface design.

  14. EHR/PHR Basics

    Science.gov (United States)

    ... Services (HHS). Some of the organizations that provide Electronic Health Records include health plans and providers. Health plans and most health-care providers are required to give you a Notice of Privacy Practices, which tells you how they keep any ...

  15. Mapping of a Novel Race Specific Resistance Gene to Phytophthora Root Rot of Pepper (Capsicum annuum) Using Bulked Segregant Analysis Combined with Specific Length Amplified Fragment Sequencing Strategy.

    Science.gov (United States)

    Xu, Xiaomei; Chao, Juan; Cheng, Xueli; Wang, Rui; Sun, Baojuan; Wang, Hengming; Luo, Shaobo; Xu, Xiaowan; Wu, Tingquan; Li, Ying

    2016-01-01

    Phytophthora root rot caused by Phytophthora capsici (P. capsici) is a serious limitation to pepper production in Southern China, with high temperature and humidity. Mapping PRR resistance genes can provide linked DNA markers for breeding PRR resistant varieties by molecular marker-assisted selection (MAS). Two BC1 populations and an F2 population derived from a cross between P. capsici-resistant accession, Criollo de Morelos 334 (CM334) and P. capsici-susceptible accession, New Mexico Capsicum Accession 10399 (NMCA10399) were used to investigate the genetic characteristics of PRR resistance. PRR resistance to isolate Byl4 (race 3) was controlled by a single dominant gene, PhR10, that was mapped to an interval of 16.39Mb at the end of the long arm of chromosome 10. Integration of bulked segregant analysis (BSA) and Specific Length Amplified Fragment sequencing (SLAF-seq) provided an efficient genetic mapping strategy. Ten polymorphic Simple Sequence Repeat (SSR) markers were found within this region and used to screen the genotypes of 636 BC1 plants, delimiting PhR10 to a 2.57 Mb interval between markers P52-11-21 (1.5 cM away) and P52-11-41 (1.1 cM). A total of 163 genes were annotated within this region and 31 were predicted to be associated with disease resistance. PhR10 is a novel race specific gene for PRR, and this paper describes linked SSR markers suitable for marker-assisted selection of PRR resistant varieties, also laying a foundation for cloning the resistance gene.

  16. Mapping of a Novel Race Specific Resistance Gene to Phytophthora Root Rot of Pepper (Capsicum annuum Using Bulked Segregant Analysis Combined with Specific Length Amplified Fragment Sequencing Strategy.

    Directory of Open Access Journals (Sweden)

    Xiaomei Xu

    Full Text Available Phytophthora root rot caused by Phytophthora capsici (P. capsici is a serious limitation to pepper production in Southern China, with high temperature and humidity. Mapping PRR resistance genes can provide linked DNA markers for breeding PRR resistant varieties by molecular marker-assisted selection (MAS. Two BC1 populations and an F2 population derived from a cross between P. capsici-resistant accession, Criollo de Morelos 334 (CM334 and P. capsici-susceptible accession, New Mexico Capsicum Accession 10399 (NMCA10399 were used to investigate the genetic characteristics of PRR resistance. PRR resistance to isolate Byl4 (race 3 was controlled by a single dominant gene, PhR10, that was mapped to an interval of 16.39Mb at the end of the long arm of chromosome 10. Integration of bulked segregant analysis (BSA and Specific Length Amplified Fragment sequencing (SLAF-seq provided an efficient genetic mapping strategy. Ten polymorphic Simple Sequence Repeat (SSR markers were found within this region and used to screen the genotypes of 636 BC1 plants, delimiting PhR10 to a 2.57 Mb interval between markers P52-11-21 (1.5 cM away and P52-11-41 (1.1 cM. A total of 163 genes were annotated within this region and 31 were predicted to be associated with disease resistance. PhR10 is a novel race specific gene for PRR, and this paper describes linked SSR markers suitable for marker-assisted selection of PRR resistant varieties, also laying a foundation for cloning the resistance gene.

  17. Aktivitas Penyembuhan Luka Sediaan Topikal Ekstrak Bawang Merah (Allium cepa terhadap Luka Sayat Kulit Mencit (Mus Musculus (THE ACTIVITY OF TOPICAL EXTRACT OF ONIONS (ALLIUM CEPA ON WOUND HEALING PROCESS IN MICE (MUS MUSCULUS

    Directory of Open Access Journals (Sweden)

    Visa Yunanda

    2017-01-01

    Full Text Available Wound healing is a physiological response of the body to restore continuity, structure and function ofthe injured tissue. Onion is one of the plants that are empirically used by the community to heal wounds.The purpose of this study was to observe the effect of topical extract of onions (Allium cepa on woundhealing process in mice (Mus musculus strain BalbC. This study used the posttest-only control groupdesign with completely randomized design (CRD. The samples were 12 male mice with weight ranged of22-32 g divided into four treatments; i.e.: Vaseline, topical extract of onion 5%, topical extract of onion30% and topical extract of onion 55% with 3 replications. Wound healing evaluated macroscopically toobserve hyperemia, wound contraction, granulation, crusting and pus production; and microscopically bycounting the number of fibroblasts on day 10. Hyperemia, granulation, crusting and pus production wereanalyzed descriptively. Wound contraction and the number of fibroblasts were analyzed using ANOVA(p<0.05 and followed with Duncan’s test (p<0,05. Descriptive observations obtained hyperemia lasteduntil day 3, granulation in wounds used topical extract of onion 30% and 55% were faster than the other,crusting lasted until day 6 and the production of pus was not found in any treatment. Statistical testresults showed that topical extract of onion 55% significantly affected wound contraction and topicalextract of onion 30% and 55% significantly affected maturation, which characterized by the decreasednumber of fibroblasts. The results of this study showed that topical extract of onion had significant effect(p<0.05 on wound healing and the effect was in line with the increased concentration of the onion extract.

  18. Femoral cartilage thickness measurements in healthy individuals: learning, practicing and publishing with TURK-MUSCULUS.

    Science.gov (United States)

    Özçakar, Levent; Tunç, Hakan; Öken, Öznur; Ünlü, Zeliha; Durmuş, Bekir; Baysal, Özlem; Altay, Zuhal; Tok, Fatih; Akkaya, Nuray; Doğu, Beril; Çapkın, Erhan; Bardak, Ayşenur; Çarlı, Alparslan Bayram; Buğdaycı, Derya; Toktaş, Hasan; Dıraçoğlu, Demirhan; Gündüz, Berrin; Erhan, Belgin; Kocabaş, Hilal; Erden, Gül; Günendi, Zafer; Kesikburun, Serdar; Omaç, Özlem Köroğlu; Taşkaynatan, Mehmet Ali; Şenel, Kazım; Uğur, Mahir; Yalçınkaya, Ebru Yılmaz; Öneş, Kadriye; Atan, Çiğdem; Akgün, Kenan; Bilgici, Ayhan; Kuru, Ömer; Özgöçmen, Salih

    2014-01-01

    Measurement of the femoral cartilage thickness by using in-vivo musculoskeletal ultrasonography (MSUS) has been previously shown to be a valid and reliable method in previous studies; however, to our best notice, normative data has not been provided before in the healthy population.The aim of our study was to provide normative data regarding femoral cartilage thicknesses of healthy individuals with collaborative use of MSUS. This is across-sectional study run at Physical and Rehabilitation Medicine Departments of 18 Secondary and Tertiary Centers in Turkey. 1544 healthy volunteers (aged between 25-40 years) were recruited within the collaboration of TURK-MUSCULUS (Turkish Musculoskeletal Ultrasonography Study Group). Subjects who had a body mass index value of less than 30 and who did not have signs and symptoms of any degenerative/inflammatory arthritis or other rheumatic diseases, history of knee trauma and previous knee surgery were enrolled. Ultrasonographic measurements were performed axially from the suprapatellar window by using linear probes while subjects' knees were in maximum flexion. Three (mid-point) measurements were taken from both knees (lateral condyle, intercondylar area, medial condyle). A total of 2876 knees (of 817 M, 621 F subjects) were taken into analysis after exclusion of inappropriate images. Mean cartilage thicknesses were significantly lower in females than males (all p< 0.001). Thickness values negatively correlated with age; negatively (females) and positively (males) correlated with smoking. Men who regularly exercised had thicker cartilage than who did not exercise (all p < 0.05). Increased age (in both sexes) and absence of exercise (males) were found to be risk factors for decreased cartilage thicknesses. Further data pertaining to other countries would be interesting to uncover whether ethnic differences also affect cartilage thickness. Collaborative use of MSUS seems to be promising in this regard.

  19. Identification and Characterization of a Novel Issatchenkia orientalis GPI-Anchored Protein, IoGas1, Required for Resistance to Low pH and Salt Stress.

    Directory of Open Access Journals (Sweden)

    Akinori Matsushika

    Full Text Available The use of yeasts tolerant to acid (low pH and salt stress is of industrial importance for several bioproduction processes. To identify new candidate genes having potential roles in low-pH tolerance, we screened an expression genomic DNA library of a multiple-stress-tolerant yeast, Issatchenkia orientalis (Pichia kudriavzevii, for clones that allowed Saccharomyces cerevisiae cells to grow under highly acidic conditions (pH 2.0. A genomic DNA clone containing two putative open reading frames was obtained, of which the putative protein-coding gene comprising 1629 bp was retransformed into the host. This transformant grew significantly at pH 2.0, and at pH 2.5 in the presence of 7.5% Na2SO4. The predicted amino acid sequence of this new gene, named I. orientalis GAS1 (IoGAS1, was 60% identical to the S. cerevisiae Gas1 protein, a glycosylphosphatidylinositol-anchored protein essential for maintaining cell wall integrity, and 58-59% identical to Candida albicans Phr1 and Phr2, pH-responsive proteins implicated in cell wall assembly and virulence. Northern hybridization analyses indicated that, as for the C. albicans homologs, IoGAS1 expression was pH-dependent, with expression increasing with decreasing pH (from 4.0 to 2.0 of the medium. These results suggest that IoGAS1 represents a novel pH-regulated system required for the adaptation of I. orientalis to environments of diverse pH. Heterologous expression of IoGAS1 complemented the growth and morphological defects of a S. cerevisiae gas1Δ mutant, demonstrating that IoGAS1 and the corresponding S. cerevisiae gene play similar roles in cell wall biosynthesis. Site-directed mutagenesis experiments revealed that two conserved glutamate residues (E161 and E262 in the IoGas1 protein play a crucial role in yeast morphogenesis and tolerance to low pH and salt stress. Furthermore, overexpression of IoGAS1 in S. cerevisiae remarkably improved the ethanol fermentation ability at pH 2.5, and at pH 2.0 in the

  20. iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.

    Directory of Open Access Journals (Sweden)

    Xiaofang Geng

    Full Text Available The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians at transcriptional level, but the initiation mechanism of planarian head regeneration (PHR remains unclear at the protein level. Here, a global analysis of proteome dynamics during the early stage of PHR was performed using isobaric tags for relative and absolute quantitation (iTRAQ-based quantitative proteomics strategy, and our data are available via ProteomeXchange with identifier PXD002100. The results showed that 162 proteins were differentially expressed at 2 h and 6 h following amputation. Furthermore, the analysis of expression patterns and functional enrichment of the differentially expressed proteins showed that proteins involved in muscle contraction, oxidation reduction and protein synthesis were up-regulated in the initiation of PHR. Moreover, ingenuity pathway analysis showed that predominant signaling pathways such as ILK, calcium, EIF2 and mTOR signaling which were associated with cell migration, cell proliferation and protein synthesis were likely to be involved in the initiation of PHR. The results for the first time demonstrated that muscle contraction and ILK signaling might played important roles in the initiation of PHR at the global protein level. The findings of this research provide a molecular basis for further unraveling the mechanism of head regeneration initiation in planarians.

  1. Ventilation criteria for IDMS facility

    International Nuclear Information System (INIS)

    Lambert, D.P.

    1996-01-01

    Both Facility Evaluation Board (FEB) reviews of the Integrated DWPF Melter System (IDMS) have identified the inconsistency of the current IDMS Process Hazards Review (PHR) versus actual IDMS practice as regards the criteria to contain air borne pollutants that may be present in the Process Room (e.g. benzene and mercury). The PHR states that a 1.0 in. wc pressure differential be maintained between the IDMS Process Room and Building 672-T. In addition, the PHR further specifies that the linear velocity through openings into the Process Room (e.g. open doors) be equal to or greater than 150 fpm. Finally, the PHR recommended that mercury vapor and benzene monitors be installed in the Process Room ventilation exhaust to alert personnel to the presence of vapors of benzene and/or mercury before entering the Process Room. This report summarizes the results of reassessment of these criteria and the specific recommendation for permanent installation of mercury and benzene vapor monitors in the vapor exhaust of the Process Room

  2. Consumer Adoption of Personal Health Record Systems: A Self-Determination Theory Perspective.

    Science.gov (United States)

    Assadi, Vahid; Hassanein, Khaled

    2017-07-27

    Personal Health Records (PHR) systems provide individuals with access and control over their health information and consequently can support individuals in becoming active participants, rather than passive recipients, in their own care process. In spite of numerous benefits suggested for consumers' utilizing PHR systems, research has shown that such systems are not yet widely adopted or well known to consumers. Bearing in mind the potential benefits of PHRs to consumers and their potential interest in these systems-and that similar to any other type of information system, adoption is a prerequisite for realizing the potential benefits of PHR systems-research is needed to understand how to enhance the adoption rates for PHR systems. This research seeks to understand how individuals' intentions to adopt PHR systems are affected by their self-determination in managing their own health-the extent of their ability to take an active role in managing their own health. As such, this research aims to develop and empirically validate a theoretical model that explains PHR systems adoption by the general public through the integration of theories from the information systems and psychology literatures. This research employs a cross-sectional survey method targeted at the Canadian general public without any prior experience in using PHR systems. A partial least squares approach to structural equation modeling was used to validate the proposed research model of this study (N=159). Individuals with higher levels of ability to manage their own health (self-determination) are more likely to adopt PHR systems since they have more positive perceptions regarding the use of such systems. Further, such self-determination is fueled by autonomy support from consumers' physicians as well as the consumers' personality trait of autonomy orientation. This study advances our theoretical understanding of PHR systems adoption. It also contributes to practice by providing insightful implications

  3. Protein: FBA7 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA7 claudin-zona occluden Tjp1 Zo1 Tight junction protein ZO-1 Tight junction protein 1, Zona occludens pr...otein 1, Zonula occludens protein 1 10090 Mus musculus 21872 P39447 2RRM P39447 21431884 ...

  4. Effect of Recycled Rubber Particles and Silica on Tensile and Tear Properties of Natural Rubber Composites

    Directory of Open Access Journals (Sweden)

    Velu CHANDRAN

    2016-05-01

    Full Text Available Application of scrap rubber and worn out tires in natural rubber compounds has been studied. The scrap rubber can, however, be recycled and compounded with natural rubber and thus can be generated as a rubber composite. In this work, recycled rubber particles (RRP were prepared using pulverization process. Then, RRP was blended with natural rubber and silica compounds, and it was synthesized by two- roll mill and hydraulic press at specified operating conditions. The samples ranging from 0 to 40 phr of RRP loaded with silica were used as constant filler. The mechanical properties and morphological analysis were carried out. The results showed that tensile strength and elongation at break gradually decreased with increasing RRP loading in natural rubber and silica compounds. Tensile modulus went down at 10 phr of RRP and then showed an increasing trend. Hardness increased up to 30 phr of RRP and tear strength increased up to 20 phr of RRP. A comparative study was also carried out with virgin natural rubber vulcanizates. The incorporation of RRP and silica up to 20 phr in natural rubber did not lower the performance of rubber articles. Morphological studies revealed that better filler dispersion, interfacial adhesion, and cross link density could increase the tensile and tear strengths.DOI: http://dx.doi.org/10.5755/j01.ms.22.2.7330

  5. Multiwalled carbon nanotubes and fluoroelastomer antistatic nanocomposite for automotive fuel system components

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seok; Park, Seong Hwan; Ha, Kiryong [Keimyung University, Daegu (Korea, Republic of); Lee, Jong Cheol [Jin-Yang Oil Seal Co., Ltd., Daegu (Korea, Republic of)

    2016-03-15

    Fluoroelastomer (FKM) composites, reinforced with multiwalled carbon nanotubes (MWNTs), were prepared by conventional method to determine the possibility of using MWNTs to develop an antistatic composite in automotive fuel systems. The results obtained from the composite containing 0-9 phr of MWNTs were compared. A 5 points increase in hardness was achieved with the addition of only 1 phr of MWNTs and 9 phr added FKM composite was increased 6.4MPa in tensile strength compared to the MWNTs unfilled FKM composite. In addition, electrical conductivity increased from 0 to 1.039 Scm{sup -1} with increase in the MWNTs concentration, and the dynamic damping property was increased in the rubbery state region accordingly. These phenomena can be explained by the MWNTs networks formed in FKM matrix. This research will therefore be useful in the development of an antistatic rubber composite for fuel system components, which are deformed or vibrated while in operation.

  6. Multiwalled carbon nanotubes and fluoroelastomer antistatic nanocomposite for automotive fuel system components

    International Nuclear Information System (INIS)

    Lee, Seok; Park, Seong Hwan; Ha, Kiryong; Lee, Jong Cheol

    2016-01-01

    Fluoroelastomer (FKM) composites, reinforced with multiwalled carbon nanotubes (MWNTs), were prepared by conventional method to determine the possibility of using MWNTs to develop an antistatic composite in automotive fuel systems. The results obtained from the composite containing 0-9 phr of MWNTs were compared. A 5 points increase in hardness was achieved with the addition of only 1 phr of MWNTs and 9 phr added FKM composite was increased 6.4MPa in tensile strength compared to the MWNTs unfilled FKM composite. In addition, electrical conductivity increased from 0 to 1.039 Scm -1 with increase in the MWNTs concentration, and the dynamic damping property was increased in the rubbery state region accordingly. These phenomena can be explained by the MWNTs networks formed in FKM matrix. This research will therefore be useful in the development of an antistatic rubber composite for fuel system components, which are deformed or vibrated while in operation.

  7. The contribution of the Y chromosome to hybrid male sterility in house mice.

    Science.gov (United States)

    Campbell, Polly; Good, Jeffrey M; Dean, Matthew D; Tucker, Priscilla K; Nachman, Michael W

    2012-08-01

    Hybrid sterility in the heterogametic sex is a common feature of speciation in animals. In house mice, the contribution of the Mus musculus musculus X chromosome to hybrid male sterility is large. It is not known, however, whether F1 male sterility is caused by X-Y or X-autosome incompatibilities or a combination of both. We investigated the contribution of the M. musculus domesticus Y chromosome to hybrid male sterility in a cross between wild-derived strains in which males with a M. m. musculus X chromosome and M. m. domesticus Y chromosome are partially sterile, while males from the reciprocal cross are reproductively normal. We used eight X introgression lines to combine different X chromosome genotypes with different Y chromosomes on an F1 autosomal background, and we measured a suite of male reproductive traits. Reproductive deficits were observed in most F1 males, regardless of Y chromosome genotype. Nonetheless, we found evidence for a negative interaction between the M. m. domesticus Y and an interval on the M. m. musculus X that resulted in abnormal sperm morphology. Therefore, although F1 male sterility appears to be caused mainly by X-autosome incompatibilities, X-Y incompatibilities contribute to some aspects of sterility.

  8. Physical and sensory characteristics of pork sausages from enzymatically modified blends of lard and rapeseed oil during storage

    DEFF Research Database (Denmark)

    Cheong, L.Z.; Zhang, H.; Nersting, L.

    2010-01-01

    Physical and sensory characteristic of pork sausages produced from enzymatic interesterified blends of lard and rapeseed oil during storage were evaluated. All three enzymatic interesterified blends (IE90, IE70 and IE50) had ratios of unsaturated to saturated fatty acids within the range of 1.......47-2.84 which is favourable for cardiovascular disease risk reduction. Blends of IE90 and IE70 were found to have suitable solid fat content, melting and crystallization profile suitable for sausages production. Sausages were produced from blends of IE90 and IE70 with different muscle types (musculus...... longissimus dorsi and musculus sternomandibularis) and processing conditions such as cooling rates and final processing temperature. Cooling rate was found to have no significant (P>0.05) effect on hardness of the sausages throughout storage. Both musculus longissimus dorsi and high final processing...

  9. Factors influencing consumer adoption of USB-based Personal Health Records in Taiwan

    Directory of Open Access Journals (Sweden)

    Jian Wen-Shan

    2012-08-01

    Full Text Available Abstract Background Usually patients receive healthcare services from multiple hospitals, and consequently their healthcare data are dispersed over many facilities’ paper and electronic-based record systems. Therefore, many countries have encouraged the research on data interoperability, access, and patient authorization. This study is an important part of a national project to build an information exchange environment for cross-hospital digital medical records carried out by the Department of Health (DOH of Taiwan in May 2008. The key objective of the core project is to set up a portable data exchange environment in order to enable people to maintain and own their essential health information. This study is aimed at exploring the factors influencing behavior and adoption of USB-based Personal Health Records (PHR in Taiwan. Methods Quota sampling was used, and structured questionnaires were distributed to the outpatient department at ten medical centers which participated in the DOH project to establish the information exchange environment across hospitals. A total of 3000 questionnaires were distributed and 1549 responses were collected, out of those 1465 were valid, accumulating the response rate to 48.83%. Results 1025 out of 1465 respondents had expressed their willingness to apply for the USB-PHR. Detailed analysis of the data reflected that there was a remarkable difference in the “usage intention” between the PHR adopters and non-adopters (χ2 =182.4, p  Conclusions Higher Usage Intentions, Perceived Usefulness and Subjective Norm of patients were found to be the key factors influencing PHR adoption. Thus, we suggest that government and hospitals should promote the potential usefulness of PHR, and physicians should encourage patients' to adopt the PHR.

  10. Investigation of spinal cerebrospinal fluid-contacting neurons expressing PKD2L1: evidence for a conserved system from fish to primates

    Science.gov (United States)

    Djenoune, Lydia; Khabou, Hanen; Joubert, Fanny; Quan, Feng B.; Nunes Figueiredo, Sophie; Bodineau, Laurence; Del Bene, Filippo; Burcklé, Céline; Tostivint, Hervé; Wyart, Claire

    2014-01-01

    Over 90 years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs) based on their morphology and location within the spinal cord. In more than 200 vertebrate species, they observed ciliated neurons around the central canal that extended a brush of microvilli into the cerebrospinal fluid (CSF). Although their morphology is suggestive of a primitive sensory cell, their function within the vertebrate spinal cord remains unknown. The identification of specific molecular markers for these neurons in vertebrates would benefit the investigation of their physiological roles. PKD2L1, a transient receptor potential channel that could play a role as a sensory receptor, has been found in cells contacting the central canal in mouse. In this study, we demonstrate that PKD2L1 is a specific marker for CSF-cNs in the spinal cord of mouse (Mus musculus), macaque (Macaca fascicularis) and zebrafish (Danio rerio). In these species, the somata of spinal PKD2L1+ CSF-cNs were located below or within the ependymal layer and extended an apical bulbous extension into the central canal. We found GABAergic PKD2L1-expressing CSF-cNs in all three species. We took advantage of the zebrafish embryo for its transparency and rapid development to identify the progenitor domains from which pkd2l1+ CSF-cNs originate. pkd2l1+ CSF-cNs were all GABAergic and organized in two rows—one ventral and one dorsal to the central canal. Their location and marker expression is consistent with previously described Kolmer–Agduhr cells. Accordingly, pkd2l1+ CSF-cNs were derived from the progenitor domains p3 and pMN defined by the expression of nkx2.2a and olig2 transcription factors, respectively. Altogether our results suggest that a system of CSF-cNs expressing the PKD2L1 channel is conserved in the spinal cord across bony vertebrate species. PMID:24834029

  11. Investigation of spinal cerebrospinal fluid-contacting neurons expressing PKD2L1: evidence for a conserved system from fish to primates

    Directory of Open Access Journals (Sweden)

    Lydia eDjenoune

    2014-05-01

    Full Text Available Over ninety years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs based on their morphology and location within the spinal cord. In more than two hundred vertebrate species, they observed ciliated neurons around the central canal that extended a brush of microvilli into the cerebrospinal fluid (CSF. Although their morphology is suggestive of a primitive sensory cell, their function within the vertebrate spinal cord remains unknown. The identification of specific molecular markers for these neurons in vertebrates would benefit the investigation of their physiological roles. PKD2L1, a transient receptor potential channel that could play a role as a sensory receptor, has been found in cells contacting the central canal in mouse. In this study, we demonstrate that PKD2L1 is a specific marker for CSF-cNs in the spinal cord of mouse (Mus musculus, macaque (Macaca fascicularis and zebrafish (Danio rerio. In these species, the somata of spinal PKD2L1+ CSF-cNs were located below or within the ependymal layer and extended an apical bulbous extension into the central canal. We found GABAergic PKD2L1-expressing CSF-cNs in all three species. We took advantage of the zebrafish embryo for its transparency and rapid development to identify the progenitor domains from which pkd2l1+ CSF-cNs originate. pkd2l1+ CSF-cNs were all GABAergic and organized in two rows—one ventral and one dorsal to the central canal. Their location and marker expression is consistent with previously described Kolmer-Agduhr cells. Accordingly, pkd2l1+ CSF-cNs were derived from the progenitor domains p3 and pMN defined by the expression of nkx2.2a and olig2 transcription factors, respectively. Altogether our results suggest that a system of CSF-cNs expressing the PKD2L1 channel is conserved in the spinal cord across bony vertebrate species.

  12. Surgical gloves fabrication using natural rubber latex vulcanized with gamma radiation

    International Nuclear Information System (INIS)

    Collantes, Hugo David Chirinos.

    1995-01-01

    Surgical gloves were manufactured by immersion coagulant method from vulcanized natural rubber latex by gamma rays at dose of 10 kGy in the air, at room temperature, using the following sensitizer vulcanization An-B 3 phr/KOH 0.2 phr. The influence of the parameter in the thickness of the surgical gloves manufacture, studied through fractional factorial designs technic, can be resumed by empirical linear correlation: y = 0.213 + 0.025 [Ca Cl 2 ] + 0.019 t. (author). 49 refs., 13 figs., 31 tabs

  13. The Nesprin family member ANC-1 regulates synapse formation and axon termination by functioning in a pathway with RPM-1 and β-Catenin.

    Science.gov (United States)

    Tulgren, Erik D; Turgeon, Shane M; Opperman, Karla J; Grill, Brock

    2014-07-01

    Mutations in Nesprin-1 and 2 (also called Syne-1 and 2) are associated with numerous diseases including autism, cerebellar ataxia, cancer, and Emery-Dreifuss muscular dystrophy. Nesprin-1 and 2 have conserved orthologs in flies and worms called MSP-300 and abnormal nuclear Anchorage 1 (ANC-1), respectively. The Nesprin protein family mediates nuclear and organelle anchorage and positioning. In the nervous system, the only known function of Nesprin-1 and 2 is in regulation of neurogenesis and neural migration. It remains unclear if Nesprin-1 and 2 regulate other functions in neurons. Using a proteomic approach in C. elegans, we have found that ANC-1 binds to the Regulator of Presynaptic Morphology 1 (RPM-1). RPM-1 is part of a conserved family of signaling molecules called Pam/Highwire/RPM-1 (PHR) proteins that are important regulators of neuronal development. We have found that ANC-1, like RPM-1, regulates axon termination and synapse formation. Our genetic analysis indicates that ANC-1 functions via the β-catenin BAR-1, and the ANC-1/BAR-1 pathway functions cell autonomously, downstream of RPM-1 to regulate neuronal development. Further, ANC-1 binding to the nucleus is required for its function in axon termination and synapse formation. We identify variable roles for four different Wnts (LIN-44, EGL-20, CWN-1 and CWN-2) that function through BAR-1 to regulate axon termination. Our study highlights an emerging, broad role for ANC-1 in neuronal development, and unveils a new and unexpected mechanism by which RPM-1 functions.

  14. Computed tomography in Duchenne type muscular dystrophy

    International Nuclear Information System (INIS)

    Kawai, Mitsuru; Kunimoto, Masanari; Motoyoshi, Yasufumi; Kuwata, Takashi; Nakano, Imaharu

    1985-01-01

    The computed tomography (CT) scan was performed on 91 Duchenne type muscular dystrophy (DMD) patients on the following four levels; (1) at the level of L3 vertebra, (2) 2-3cm above the symphysis pubica, (3) midposition of the thigh, (4) largest-diameter section of the lower leg. The CT of muscles common to most of the DMD patients were as follows: 1. Muscle atrophy: Muscle atrophy was shown as a reduction in the cross-sectional area of the muscles. Very mild muscle atrophy could be detected either by the clearly identified muscle border or by scattered low-density areas of so-called ''moth-eaten'' appearance within muscles. 2. Fat infiltration: The decrease in radio-density of muscles was interpreted as infiltration of fatty tissue. This type of density change was further classified into diffuse, streaked, cobblestone and salt-and-pepper patterns according to the spacial distribution of low-density areas. 3. Selectivity pattern: As the chronological sequence of DMD muscle degeneration is usually different among individual muscles, it may be seen, in some stages, that some of the synergistic muscles are still only slightly involved, while the others are quite severely atrophied with evident fat infiltration. In certain stages of the disease, most of the patients show relative preservation of particular muscles although they assumed a rounded shape. The most resistent muscle was musculus gracilis, followed by the musculus sartorius, musculus semitendinosus (and/or musculus semimembranosus) in that order. According to the severity of the CT changes, 86 of the 91 patients were classed into five stages from A1 to A5. Morphological stages (A1-A5) were well correlated to the functional disability stages by Ueda with a correlation factor of r=0.88. (J.P.N.)

  15. Development of rubber gloves by radiation vulcanization

    International Nuclear Information System (INIS)

    Makuuchi, K.; Yoshii, F.; Ishigaki, I.; Mogi, M.; Saito, T.

    1990-01-01

    The processes of radiation vulcanization and production of protective rubber gloves for radioactive contamination are described. A newly developed sensitizing system consisting of 5 phr 2-ethylhexyl acrylate and 1 phr carbon tetrachloride was used to vulcanize natural rubber latex at 12 kGy. Transparent and soft gloves were obtained from the radiation vulcanized latex by a coagulant dipping process. The mechanical properties of the gloves meet Japanese Industrial Standard specification for protective gloves. Combustion analysis of the gloves revealed that the amount of evolved sulfur dioxide and remaining ashes are less than those from commercially available rubber gloves. A trial usage of the gloves at a nuclear power plant showed that the gloves were easy to use for delicate work without undergoing fatigue. (author)

  16. Defense-in-depth evaluation for the New Waste Transfer Facility

    International Nuclear Information System (INIS)

    Hayes, T.G.; Kelly, J.L.

    1995-01-01

    This report fulfills part of the requirements of References 2 and 3 by documenting a Defense-In-Depth evaluation for the New Waste Transfer Facility (NWTF). This evaluation was performed using methodology similar to that used in an evaluation for the Defense Waste Processing Facility (DWPF). It differs because the DWPF evaluation was based on an existing Process Hazards Analysis (PHA) while NWTF's is based on a Preoperational Process Hazards Review (PHR) (Ref. 1). The accidents in the Process Hazards Review (PHR) were reviewed to determine those that might have significant consequences. Significance was based on the findings of the PHR, The facility design was reviewed to determine the Structures, Systems, and Components (SSCs) and administrative controls available before and after each accident. From this was developed a list of the Lines of Defense (LODs) available to contain the hazard associated with the accident. A summary of these LODs is given in Appendix C. Items are tabulated that are suggested for consideration in the functional classification as worker protection items. The specific criteria used in the evaluation is given in the methodology section of this report. The results are documented in Appendices A, B, C, and D

  17. Obfuscatable multi-recipient re-encryption for secure privacy-preserving personal health record services.

    Science.gov (United States)

    Shi, Yang; Fan, Hongfei; Xiong, Guoyue

    2015-01-01

    With the rapid development of cloud computing techniques, it is attractive for personal health record (PHR) service providers to deploy their PHR applications and store the personal health data in the cloud. However, there could be a serious privacy leakage if the cloud-based system is intruded by attackers, which makes it necessary for the PHR service provider to encrypt all patients' health data on cloud servers. Existing techniques are insufficiently secure under circumstances where advanced threats are considered, or being inefficient when many recipients are involved. Therefore, the objectives of our solution are (1) providing a secure implementation of re-encryption in white-box attack contexts and (2) assuring the efficiency of the implementation even in multi-recipient cases. We designed the multi-recipient re-encryption functionality by randomness-reusing and protecting the implementation by obfuscation. The proposed solution is secure even in white-box attack contexts. Furthermore, a comparison with other related work shows that the computational cost of the proposed solution is lower. The proposed technique can serve as a building block for supporting secure, efficient and privacy-preserving personal health record service systems.

  18. NCBI nr-aa BLAST: CBRC-PTRO-04-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-04-0004 sp|Q8VBS7|VN1A6_MOUSE Vomeronasal type-1 receptor A6 (Vomeronasal... type-1 receptor A3) (Vomeronasal type-1 receptor A1) (Vomeronasal type-1 receptor A10) dbj|BAB79209.1| vomeronasa...l receptor 1 A1 [Mus musculus] dbj|BAB79210.1| vomeronasal receptor 1 A3 [Mus musculus] gb|AAK98772.1| vomeronasa

  19. AcEST: BP913064 [AcEST

    Lifescience Database Archive (English)

    Full Text Available _MOUSE Experimental autoimmune prostatitis antige... 31 5.2 sp|Q9NVV0|TM38B_HUMAN...|Q6QR59|EAPA2_MOUSE Experimental autoimmune prostatitis antigen 2 OS=Mus musculus GN=Eapa2 PE=2 SV=1 Length

  20. Blue Whale (Balaenoptera musculus Behavior and Group Dynamics as Observed from an Aircraft off Southern California

    Directory of Open Access Journals (Sweden)

    Kate Lomac-MacNair

    2016-02-01

    Full Text Available Group behavior and interactions of endangered blue whales (Balaenoptera musculus have not been systematically studied. Such behavioral data are often overlooked when assessing anthropogenic effects. Yet behavioral data are necessary to compare “normal” behaviors with behavior affected by anthropogenic factors of concern relative to effective management and recovery of blue whales. For a baseline study, we hypothesized that the response variables sighting rate, group size, calf presence and group cohesion (i.e., spacing between individuals within a group differed according to the spatio-temporal explanatory variables behavioral state, time of day, season, water depth and distance from shore. To address our hypotheses, we flew systematic line transect surveys in southern California and collected focal group data. Two sets of data were separately analyzed using different sampling approaches: (1 point sample data associated with the first sighting of a blue whale(s, and (2 extended all-occurrence focal group behavioral sampling data (i.e., focal follows collected on a subsample of all sightings while the aircraft circled at a radial distance of approximately 0.5-1 km and an altitude of 1,500 m for extended periods of 5 – 60 minutes. Chi-square contingency table and G² analyses were used to assess statistical relationships between response and explanatory variables. We conducted 18 one-week-long aerial surveys spanning October 2008 through May 2013 (at least once during every month except December, totaling 87,555 km of observation effort. Seventy blue whale sightings (117 individuals were seen, ranging in size from 1 – 6 whales, and focal follow was performed on over half (55% of these sightings. Results supported our hypotheses that blue whale group characteristics were related to behavioral state and spatio-temporal variables. Sighting rates were significantly highest during summer followed by spring, fall, and winter. Group type differed

  1. Pengaruh Pemberian Ekstrak Etanol Daun Kecapi (Sandoricum koetjape (Burm.f. Merr. Terhadap Penurunan Kadar Kolesterol Total pada Mencit Jantan (Mus musculus

    Directory of Open Access Journals (Sweden)

    Rudi Kartika

    2016-06-01

    Full Text Available The research about utilization of ethanol extract form leafkecapi (Sandoricum koetjape (Burm.f.Merr.has been done, it proved could decrease  total cholesterol levels in male mice (Mus musculus. In this research sample macerated with ethanol 96% and then concentrated by rotary evaporator, and gained 15.6% of yield. Based on the results of phytochemical test, secondary metabolites compound that contained in bark kecapi are alkaloid, flavonoid, triterpenoid, steroid, phenolic and saponin. On antihiperkolesterolemia activity test by using male mice, there are several treatments that negative control (-, positive control (+ and three treatment with different doses i.e. a low doses (9.5 mg/kg BB, middle doses (19 mg/kg BB and high doses (38 mg/kg BB. The parameter measured was blood serum of male mice by using a Cholesterol Test Strips. From this test it showed that the effectiveness of decreased total cholesterol level doses of 19 mg/kg BB showed total cholesterol level under 100 mg/dL. Keywords:      Leaf kecapi (Sandoricum koetjape (Burm.f. Merr., Phytochemicals test, Total cholesterol levels, Cholesterol test strips.

  2. AcEST: BP917335 [AcEST

    Lifescience Database Archive (English)

    Full Text Available _MOUSE DNA-binding protein SMUBP-2 OS=Mus musculus GN=Ighmbp2 PE=1 SV=1 Length = 993 Score = 42.4 bits (98),...THGEYTSAAE 635 >tr|Q9EQN5|Q9EQN5_RAT Antifreeze-enhancer binding protein AEP OS=Rattus norvegicus GN=Ighmbp2

  3. The Effect of Nano Loading and Ultrasonic Compounding of EVA/LDPE/Nano-magnesium Hydroxide on Mechanical Properties and Distribution of Nano Particles

    Science.gov (United States)

    Azman, I. A.; Salleh, R. M.; Alauddin, S. M.; Shueb, M. I.

    2018-05-01

    Blends of Ethylene Vinyl Acetate (EVA) and Low-Density Polyethylene (LDPE) are promising composite which have good mechanical properties to environmental stress cracking. However, they lack fire resistant properties, which limits it usage in wire and cable industry. In order to improve flame retardancy ability, a range of nano-magnesium hydroxide (nano-MH) loading which is from 0 phr to maximum of 20 phr with ultrasonic extrusion 0-100 kHz frequencies have been introduced. Ultrasonic extrusion was used to improve the distribution of nano-MH. It was found that, 10 phr of nano loading with 100 kHz ultrasonic assisted has greater tensile strength compared to the nanocomposite without ultrasonication. Further increase of nano MH loading, will decrease the tensile properties. Better elongation at break was observed at10 phr nano-MH with the frequency of 50 kHz. The sample of 20 phr of nanoMH assisted with 50 kHz ultrasonic exhibits good flexural properties while 10 phr of nano-MH without the ultrasonic assisted demonstrates good in izod impact properties. From the evaluation of mechanical properties studied, it was found that 10 phr of nano-MH has shown the best performance among all the samples tested for EVA/LDPE/nano-MH composites. Transmission Electron Microscopy (TEM) has been conducted on 10 phr sample with different frequencies in order to observe the distribution of nano-MH particles. The sample with 100 kHz frequency shows more uniform dispersion of nano-MH in EVA/LDPE composites. This investigation indicates that the ultrasonic technology can enhance the mechanical properties studied as well as the dispersion of nano particles in the composite.

  4. Genetic dissection of a key reproductive barrier between nascent species of house mice.

    Science.gov (United States)

    White, Michael A; Steffy, Brian; Wiltshire, Tim; Payseur, Bret A

    2011-09-01

    Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice.

  5. Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

    Science.gov (United States)

    White, Michael A.; Steffy, Brian; Wiltshire, Tim; Payseur, Bret A.

    2011-01-01

    Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice. PMID:21750261

  6. The military health system's personal health record pilot with Microsoft HealthVault and Google Health.

    Science.gov (United States)

    Do, Nhan V; Barnhill, Rick; Heermann-Do, Kimberly A; Salzman, Keith L; Gimbel, Ronald W

    2011-01-01

    To design, build, implement, and evaluate a personal health record (PHR), tethered to the Military Health System, that leverages Microsoft® HealthVault and Google® Health infrastructure based on user preference. A pilot project was conducted in 2008-2009 at Madigan Army Medical Center in Tacoma, Washington. Our PHR was architected to a flexible platform that incorporated standards-based models of Continuity of Document and Continuity of Care Record to map Department of Defense-sourced health data, via a secure Veterans Administration data broker, to Microsoft® HealthVault and Google® Health based on user preference. The project design and implementation were guided by provider and patient advisory panels with formal user evaluation. The pilot project included 250 beneficiary users. Approximately 73.2% of users were Microsoft® HealthVault, and 81 (32.4%) selected Google® Health as their PHR of preference. Sample evaluation of users reflected 100% (n = 60) satisfied with convenience of record access and 91.7% (n = 55) satisfied with overall functionality of PHR. Key lessons learned related to data-transfer decisions (push vs pull), purposeful delays in reporting sensitive information, understanding and mapping PHR use and clinical workflow, and decisions on information patients may choose to share with their provider. Currently PHRs are being viewed as empowering tools for patient activation. Design and implementation issues (eg, technical, organizational, information security) are substantial and must be thoughtfully approached. Adopting standards into design can enhance the national goal of portability and interoperability.

  7. Comparison the physicochemical quality indicators of Musculus Longissimus Dorsi from Mangalitsa Breed and their crossbreeds

    Directory of Open Access Journals (Sweden)

    Ondrej Debreceni

    2016-12-01

    Full Text Available In recent years, there has been a current trend in the market of pork to create products based on traditional, regional specialities, where is used technology such as drying, smoking and fermentation of products. These products require a specific quality of meat from pure-bred indigenous breeds or their crossbreeds with emphasis on dry matter content, intramuscular fat content in meat and fatty acid composition with higher share of unsaturated fatty acids and essential fatty acids. Due to this fact, indigenous breed such as the Mangalitsa has received attention from the aspect of high meat quality and meat products compared to pig meat breeds. The aim of study was to compare the meat quality of Musculus longissimus dorsi from Mangalitsa breed, the crossbreeds Mangalitsa x Duroc and the pig meat breed Slovak Large White. The experimental material comprised of 28 pcs of pigs, which were reared in the same intensive conditions and they were fed ad libitum by complete feed mixtures for fatteners. The fattening period lasted from 30 kg to 100 kg of body weight. In the presented study was found that the crossbreeds Mangalitsa x Duroc had lighter colour of meat and the Mangalitsa had darker colour than Slovak Large White (P < 0.01. From the point of texture of meat, it was found stiffer meat from Slovak Large White and more tender meat from crossbreeds Mangalitsa x Duroc compared to meat of Mangalitsa (P < 0.01. The crossbreeds Mangalitsa x Duroc had the highest intramuscular fat content and cholesterol content in meat (P < 0.01. The intramuscular fat content and cholesterol content was the lowest in Slovak Large White (P < 0.01. The results indicate that utilization of Mangalitsa breed for crossing with pig meat breeds can improve quality meat traits in their crossbreeds, which are requiring for production of special meat products.

  8. Radiation vulcanization of natural rubber latex with low energy accelerator-II

    Energy Technology Data Exchange (ETDEWEB)

    Haque, Md. Emdadul; Makuuchi, Keizo; Ikeda, Kenichi; Yoshii, Fumio; Kume, Tamikazu [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment; Mitomo, Hiroshi [Gunma Univ., Faculty of Engineering, Dept. of Biological and Chemical Engineering, Kiryu, Gunma (Japan)

    2002-03-01

    The natural rubber latex (NRL) was radiation vulcanized under a low energy electron accelerator. Accelerating voltage and maximum beam current of this accelerator are 250 kV and 10 mA respectively. Irradiation was carried out in a reaction vessel with constant stirring. The capacity of the vessel is 18 liters. Radiation vulcanization accelerators (RVA) were normal butyl acrylate (n-BA) and nonane-diol-diacrylate (NDDA). NDDA has no bad smell like that of n-BA. 20 minutes irradiation time is enough to vulcanize 14 liters of latex when 5 phr RVA (both types) are used. Maximum of {approx}30 MPa tensile strength was obtained with 5 phr NDD-A. However the remained NDDA is difficult to remove due to high molecular weight. Water-extractable proteins content was determined in dipped films for various leaching conditions without and with additive (polyvinyl alcohol, PVA). Water extractable proteins content is reduced to {<=} 41 by adding 5 phr PVA and leaching for 8 hours. The tackiness of the dipped films is reduced to 0.1 from 9 gf by mixing 6 phr PVA with the irradiated latex. Hand gloves (surgical and examination) were successfully produced from the irradiated latex. (author)

  9. Importance-Performance Analysis of Personal Health Records in Taiwan: A Web-Based Survey

    Science.gov (United States)

    Rau, Hsiao-Hsien; Chen, Kang-Hua

    2017-01-01

    Background Empowering personal health records (PHRs) provides basic human right, awareness, and intention for health promotion. As health care delivery changes toward patient-centered services, PHRs become an indispensable platform for consumers and providers. Recently, the government introduced “My health bank,” a Web-based electronic medical records (EMRs) repository for consumers. However, it is not yet a PHR. To date, we do not have a platform that can let patients manage their own PHR. Objective This study creates a vision of a value-added platform for personal health data analysis and manages their health record based on the contents of the "My health bank." This study aimed to examine consumer expectation regarding PHR, using the importance-performance analysis. The purpose of this study was to explore consumer perception regarding this type of a platform: it would try to identify the key success factors and important aspects by using the importance-performance analysis, and give some suggestions for future development based on it. Methods This is a cross-sectional study conducted in Taiwan. Web-based invitation to participate in this study was distributed through Facebook. Respondents were asked to watch an introductory movie regarding PHR before filling in the questionnaire. The questionnaire was focused on 2 aspects, including (1) system functions, and (2) system design and security and privacy. The questionnaire would employ 12 and 7 questions respectively. The questionnaire was designed following 5-points Likert scale ranging from 1 (“disagree strongly”) to 5 (“Agree strongly”). Afterwards, the questionnaire data was sorted using IBM SPSS Statistics 21 for descriptive statistics and the importance-performance analysis. Results This research received 350 valid questionnaires. Most respondents were female (219 of 350 participants, 62.6%), 21-30 years old (238 of 350 participants, 68.0%), with a university degree (228 of 350 participants, 65.1

  10. Synthesis of a novel hydantoin-containing silane and its effect on the tracking and bacteria resistance of addition-cure liquid silicone rubber

    Science.gov (United States)

    Liu, Tian; Zeng, Xingrong; Fang, Weizhen; Lai, Xuejun; Li, Hongqiang

    2017-11-01

    A novel hydantoin-containing silane, [3-(5,5-dimethylhydantoinurethano) propyl] ethoxyallyloxysilane (DMHURPAS), was synthesized and the structure was characterized by FTIR and 1H NMR. The effect of DMHURPAS was investigated on the anti-tracking and antibacterial properties of addition-cure liquid silicone rubber (ALSR) after surface chlorination. It was found that ALSR containing only 1.5 phr of DMHURPAS passed 1A 4.5 kV level and erosion mass decreased from 0.843 g to 0.037 g. The thermal stability of ALSR was significantly improved and the mechanical properties were also enhanced. From thermogravimetry-Fourier transform infrared spectroscopy (TG-FTIR), ALSR/DMHURPAS showed significant decrease of carbonyl compounds and cyclic oligomers but increase of CH4 and CO2 during thermal degradation, indicating that DMHURPAS could inhibit oxidation of methyl groups and unzipping reaction, and promote the cleavage of methyl groups in ALSR. The antibacterial rates of ALSR containing 2.0 phr of DMHURPAS against Escherichia coli and Staphylococcus aureus were 95.7% and 83.4%, respectively.

  11. Genotoxic effects in wild rodents (Rattus rattus and Mus musculus) in an open coal mining area.

    Science.gov (United States)

    León, Grethel; Pérez, Lyda Espitia; Linares, Juan Carlos; Hartmann, Andreas; Quintana, Milton

    2007-06-15

    Coal is a mixture of a variety of compounds containing mutagenic and carcinogenic polycyclic aromatic hydrocarbons. Exposure to coal is considered as an important non-cellular and cellular source of reactive oxygen species that can induce DNA damage. In addition, spontaneous combustion can occur in coal mining areas, further releasing compounds with detrimental effects on the environment. In this study the comet assay was used to investigate potential genotoxic effects of coal mining activities in peripheral blood cells of the wild rodents Rattus rattus and Mus musculus. The study was conducted in a coal mining area of the Municipio de Puerto Libertador, South West of the Departamento de Cordoba, Colombia. Animals from two areas in the coal mining zone and a control area located in the Municipio de Lorica were investigated. The results showed evidence that exposure to coal results in elevated primary DNA lesions in blood cells of rodents. Three different parameters for DNA damage were assessed, namely, DNA damage index, migration length and percentage damaged cells. All parameters showed statistically significantly higher values in mice and rats from the coal mining area in comparison to the animals from the control area. The parameter "DNA Damage Index" was found to be most sensitive and to best indicate a genotoxic hazard. Both species investigated were shown to be sensitive indicators of environmental genotoxicity caused by coal mining activities. In summary, our study constitutes the first investigation of potential genotoxic effects of open coal mining carried out in Puerto Libertador. The investigations provide a guide for measures to evaluate genotoxic hazards, thereby contributing to the development of appropriate measures and regulations for more careful operations during coal mining.

  12. Circumpolar diversity and geographic differentiation of mtDNA in the critically endangered Antarctic blue whale (Balaenoptera musculus intermedia.

    Directory of Open Access Journals (Sweden)

    Angela L Sremba

    Full Text Available The Antarctic blue whale (Balaenoptera musculus intermedia was hunted to near extinction between 1904 and 1972, declining from an estimated initial abundance of more than 250,000 to fewer than 400. Here, we describe mtDNA control region diversity and geographic differentiation in the surviving population of the Antarctic blue whale, using 218 biopsy samples collected under the auspices of the International Whaling Commission (IWC during research cruises from 1990-2009. Microsatellite genotypes and mtDNA sequences identified 166 individuals among the 218 samples and documented movement of a small number of individuals, including a female that traveled at least 6,650 km or 131° longitude over four years. mtDNA sequences from the 166 individuals were aligned with published sequences from 17 additional individuals, resolving 52 unique haplotypes from a consensus length of 410 bp. From this minimum census, a rarefaction analysis predicted that only 72 haplotypes (95% CL, 64, 86 have survived in the contemporary population of Antarctic blue whales. However, haplotype diversity was relatively high (0.968±0.004, perhaps as a result of the longevity of blue whales and the relatively recent timing of the bottleneck. Despite the potential for circumpolar dispersal, we found significant differentiation in mtDNA diversity (F(ST = 0.032, p<0.005 and microsatellite alleles (F(ST = 0.005, p<0.05 among the six Antarctic Areas historically used by the IWC for management of blue whales.

  13. AcEST: DK951349 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Forkhead box protein J3 OS=Mus musculus GN... 33 1.5 sp|Q9UIG4|PS1C2_HUMAN Psoriasis...se kinase kin... 32 2.6 sp|Q7YR45|PS1C2_PANTR Psoriasis susceptibility 1 candidat...PQQHSQLQPPHSQHPPPHQH 424 >sp|Q9UIG4|PS1C2_HUMAN Psoriasis susceptibility 1 candidate gene 2 protein OS=Homo

  14. A Comparison of mucosal surface area and villous histology in small intestines of the Brazilian free-tailed bat (Tadarida brasiliensis) and the mouse (Mus musculus).

    Science.gov (United States)

    Zhang, Zhi-Qiang; Brun, Antonio; Price, Edwin R; Cruz-Neto, Ariovaldo P; Karasov, William H; Caviedes-Vidal, Enrique

    2015-01-01

    Studies on birds have led to the hypothesis that increased intestinal absorption between enterocytes (paracellular) evolved as a compensation for smaller intestinal size in fliers, which was perhaps selected to minimize the mass of digesta carried. This hypothesis predicts that bats will also exhibit relatively reduced intestinal size and high paracellular absorption, compared with nonflying mammals. Published studies on three bat species indicate relatively high paracellular absorption. One mechanism for increasing paracellular absorption per cm2 small intestine (SI) is increased number of tight junctions (TJs) across which paracellular absorption occurs. To our knowledge, we provide the first comparative analysis of enterocyte size and number in flying and nonflying mammals. Intestines of insectivorous bats Tadarida brasiliensis were compared with Mus musculus using hematoxylin and eosin staining method. Bats had shorter and narrower SIs than mice, and after correction for body size difference by normalizing to mass3/4, the bats had 40% less nominal surface area than the mouse, as predicted. Villous enhancement of surface area was 90% greater in the bat than in the mouse, mainly because of longer villi and a greater density of villi in bat intestines. Bat and mouse were similar in enterocyte diameter. Bats exceeded mice by 54.4% in villous area per cm length SI and by 95% in number of enterocytes per cm2 of the nominal surface area of the SI. Therefore, an increased density of TJs per cm2 SI may be a mechanistic explanation that helps to understand the high paracellular absorption observed in bats compared to nonflying mammals. © 2014 Wiley Periodicals, Inc.

  15. Effect of concentration of polyfunctional monomers on physical properties of acrylonitrile-butadiene rubber under electron-beam irradiation

    International Nuclear Information System (INIS)

    Yasin, T.; Ahmed, S.; Yoshii, F.; Makuuchi, K.

    2003-01-01

    The effect of concentration of different polyfunctional monomers (PFMs) on the physical properties of electron-beam irradiated acrylonitrile-butadiene rubber (NBR) has been investigated. The PFMs used were diethylene glycol dimethacrylate (2G), tetraethylene glycol dimethacrylate (4G), trimethylol propane triacrylate (A-TMPT), trimethylol propane trimethacrylate (TMPT) and tetramethylol methane tetraacrylate (A-TMMT). The physical properties of EB irradiated NBR sheets were evaluated by measurement of tensile strength, elongation %, hardness and gel fraction etc. The results show a remarkable increase in all physical properties as the concentration of PFMs increases from 1 phr to 5 phr in the NBR samples. The improvement in physical properties of radiation crosslinked NBR in the presence of PFMs may be attributed to its increased crosslinking density as observed by corresponding increase in gel content

  16. In-plane magnetic anisotropy and temperature dependence of switching field in (Ga, Mn) as ferromagnetic semiconductors.

    Science.gov (United States)

    Kamara, S; Terki, F; Dumas, R; Dehbaoui, M; Sadowski, J; Galéra, R M; Tran, Q-H; Charar, S

    2012-06-01

    We explore the magnetic anisotropy of GaMnAs ferromagnetic semiconductor by Planar Hall Effect (PHE) measurements. Using low magnitude of applied magnetic field (i.e., when the magnitude H is smaller than both cubic Hc and uniaxial Hu anisotropy field), we have observed various shapes of applied magnetic field direction dependence of Planar Hall Resistance (PHR). In particular, in two regions of temperature. At T Tc/2 the "zigzag-shape" signal of PHR. They reflect different magnetic anisotropy and provide information about magnetization reversal process in GaMnAs ferromagnetic semiconductor. The theoretical model calculation of PHR based on the free energy density reproduces well the experimental data. We report also the temperature dependence of anisotropy constants and magnetization orientations. The transition of easy axis from biaxial to uniaxiale axes has been observed and confirmed by SQUID measurements.

  17. Mechanical Properties of Graphene-Rubber Nanocomposites

    Science.gov (United States)

    Anhar, N. A. M.; Ramli, M. M.; Hambali, N. A. M. A.; Aziz, A. A.; Mat Isa, S. S.; Danial, N. S.; Abdullah, M. M. A. B.

    2017-11-01

    This research focused on development of wearable sensor device by using Prevulcanized Natural Rubber (PV) and Epoxidized Natural Rubber (ENR 50) latex incorporated with graphene oxide (GO), graphene paste, graphene powder and reduced graphene oxide (rGO) powder. The compounding formulation and calculation were based on phr (parts per hundred rubber) and all the samples were then tested for mechanical properties using Instron 5565 machine. It was found that the sonication effects on tensile strength may have better quality of tensile strength compared to non-sonicated GO. For PV incorporate GO, the optimum loading was best determined at loading 1.5 phr with or without sonication and similar result was recorded for PV/G. For ENR 50 incorporate graphene paste and rGO powder nanocomposite shows the best optimum was at 3.0 phr with 24 hours’ sonication.

  18. Biometry by ultrasonography of the epaxial and pelvic musculature in equines trained with Pessoa's rein

    Directory of Open Access Journals (Sweden)

    Kátia de Oliveira

    2014-11-01

    Full Text Available This study aimed to evaluate the use of Pessoa's rein in training equine, as a support in exercise training, on biometry by ultrasonography of the epaxial (Longíssimus Dorsi, Gluteus Medius e Musculus Multifidus and pelvic musculature (Biceps Femoris e Semitendinosus. Thereby, eight Quarter Horse mares was used, with eight years on average age, 400kg of body weight, trained with the Pessoa's rein twice a week, for two months. Variables were measured before and after training, consisting of assessment thickness (cm of the Longíssimus Dorsi and cross-sectional area (cm2 of muscles, Gluteus Medius, Biceps Femoris, Semitendinosus and Musculus Multifidus. It observed a significant effect of training with Pessoa's rein, on the Biceps Femoris (P<0.01 and Musculus Multifidus (P<0.01, which the average cross-sectional area at the final evaluation were of 28.66cm2 and 14.29cm2, respectively. Thus it can be conclude that training with Pessoa's rein modifies muscular function, promoting hypertrophy Musculus Multifidus and Biceps Femoris of horses

  19. Genetic conflict outweighs heterogametic incompatibility in the mouse hybrid zone?

    Directory of Open Access Journals (Sweden)

    Dufková Petra

    2008-10-01

    Full Text Available Abstract Background The Mus musculus musculus/M. m. domesticus contact zone in Europe is characterised by sharp frequency discontinuities for sex chromosome markers at the centre of wider clines in allozyme frequencies. Results We identify a triangular area (approximately 330 km2 where the musculus Y chromosome introgresses across this front for up to 22 km into domesticus territory. Introgression of the Y chromosome is accompanied by a perturbation of the census sex ratio: the sex ratio is significantly female biased in musculus localities and domesticus localities lacking Y chromosome introgression. In contrast, where the musculus Y is detected in domesticus localities, the sex ratio is close to parity, and significantly different from both classes of female biased localities. The geographic position of an abrupt cline in an X chromosome marker, and autosomal clines centred on the same position, seem unaffected by the musculus Y introgression. Conclusion We conclude that sex ratio distortion is playing a role in the geographic separation of speciation genes in this section of the mouse hybrid zone. We suggest that clines for genes involved in sex-ratio distortion have escaped from the centre of the mouse hybrid zone, causing a decay in the barrier to gene flow between the two house mouse taxa.

  20. Dicty_cDB: Contig-U15993-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available liant... 48 0.74 1 ( BQ829038 ) LL6in20026 AFT024-subtracted library Mus musculus... 48 0.74 1 ( BQ550344 ) ...RIKEN fu... 48 0.74 1 ( W28637 ) 49g3 Human retina cDNA randomly primed sublibrary H... 48 0.74 1 ( FK711462...ixis FlyTag MN08 BlueScript Dr... 50 0.19 1 ( CF879159 ) tric019xf19.b13 T.reesei mycelial culture, Versio...... 50 0.19 1 ( CF869496 ) tric019xi11.b1 T.reesei mycelial culture, Version... 50 ...-G09.y1d-s SHGC-CDA Gasterosteus aculeatus c... 50 0.19 1 ( CB899638 ) tric019xi11 T.reesei mycelial culture

  1. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs

    Czech Academy of Sciences Publication Activity Database

    Kváč, Martin; Kestřánová, M.; Květoňová, Dana; Kotková, M.; Ortega, Y.; McEvoy, J.; Sak, Bohumil

    2012-01-01

    Roč. 131, č. 1 (2012), s. 107-110 ISSN 0014-4894 R&D Projects: GA MŠk(CZ) LH11061 Grant - others:GA ČR(CZ) GA206/08/0640 Program:GA Institutional support: RVO:60077344 Keywords : Pigs * Cryptosporidium tyzzeri * Cryptosporidium muris * Experimental infection * PCR * Histology Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.154, year: 2012 http://www.sciencedirect.com/science/article/pii/S0014489412001105

  2. Migratory movements of pygmy blue whales (Balaenoptera musculus brevicauda between Australia and Indonesia as revealed by satellite telemetry.

    Directory of Open Access Journals (Sweden)

    Michael C Double

    Full Text Available In Australian waters during the austral summer, pygmy blue whales (Balaenoptera musculus brevicauda occur predictably in two distinct feeding areas off western and southern Australia. As with other blue whale subspecies, outside the austral summer their distribution and movements are poorly understood. In order to describe the migratory movements of these whales, we present the satellite telemetry derived movements of eleven individuals tagged off western Australia over two years. Whales were tracked from between 8 and 308 days covering an average distance of 3,009±892 km (mean ± se; range: 832 km-14,101 km at a rate of 21.94±0.74 km per day (0.09 km-455.80 km/day. Whales were tagged during March and April and ultimately migrated northwards post tag deployment with the exception of a single animal which remained in the vicinity of the Perth Canyon/Naturaliste Plateau for its eight day tracking period. The tagged whales travelled relatively near to the Australian coastline (100.0±1.7 km until reaching a prominent peninsula in the north-west of the state of Western Australia (North West Cape after which they travelled offshore (238.0±13.9 km. Whales reached the northern terminus of their migration and potential breeding grounds in Indonesian waters by June. One satellite tag relayed intermittent information to describe aspects of the southern migration from Indonesia with the animal departing around September to arrive in the subtropical frontal zone, south of western Australia in December. Throughout their migratory range, these whales are exposed to impacts associated with industry, fishing and vessel traffic. These movements therefore provide a valuable tool to industry when assessing potential interactions with pygmy blue whales and should be considered by conservation managers and regulators when mitigating impacts of development. This is particularly relevant for this species as it continues to recover from past exploitation.

  3. NCBI nr-aa BLAST: CBRC-RNOR-08-0336 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-08-0336 ref|NP_666341.1| dynein cytoplasmic 1 light intermediate chain 1 ...[Mus musculus] sp|Q8R1Q8|DC1L1_MOUSE Cytoplasmic dynein 1 light intermediate chain 1 (Dynein light intermedia...te chain 1, cytosolic) (Dynein light chain A) (DLC-A) gb|AAH23347.1| Dynein cytoplasmic 1 light intermediat...b|EDL09299.1| dynein cytoplasmic 1 light intermediate chain 1 [Mus musculus] NP_666341.1 1e-137 63% ...

  4. Digital divide: Use of electronic personal health record by different population groups.

    Science.gov (United States)

    Kim, Eung-Hun; Kim, Yongmin

    2010-01-01

    Personal Health Record (PHR) has been increasingly recognized and actively promoted by the federal government, experts and industry as an important tool for improving healthcare in the U.S. However, the PHR use by patients and its utility have not been studied well. We have evaluated a web-based PHR in multiple locations covering diverse population groups. The study sites included a surgical specialty clinic, a medical specialty clinic, and a mental health clinic at the University of Washington, and a low-income elderly housing facility near Seattle in the state of Washington. The PHR use by the low-income elderly was limited due to poor technical skills and low physical/cognitive abilities. On the other hand, the younger and affluent populations used the web-based PHR much easily and efficiently compared to the older and low-income group. They regarded managing personal health information easy while the older group struggled. As more computer literate individuals age, the next-generation elderly are certain to be more technically skilled than the current generation. Although the reduced physical/cognitive abilities due to aging would still be a challenge, more elderly people will be able to not only use a PHR system but also use it to the full extent to get the maximum benefit.

  5. Cryo-EM structures of the mammalian endo-lysosomal TRPML1 channel elucidate the combined regulation mechanism

    Directory of Open Access Journals (Sweden)

    Sensen Zhang

    2017-09-01

    Full Text Available Abstract TRPML1 channel is a non-selective group-2 transient receptor potential (TRP channel with Ca2+ permeability. Located mainly in late endosome and lysosome of all mammalian cell types, TRPML1 is indispensable in the processes of endocytosis, membrane trafficking, and lysosome biogenesis. Mutations of TRPML1 cause a severe lysosomal storage disorder called mucolipidosis type IV (MLIV. In the present study, we determined the cryo-electron microscopy (cryo-EM structures of Mus musculus TRPML1 (mTRPML1 in lipid nanodiscs and Amphipols. Two distinct states of mTRPML1 in Amphipols are added to the closed state, on which could represent two different confirmations upon activation and regulation. The polycystin-mucolipin domain (PMD may sense the luminal/extracellular stimuli and undergo a “move upward” motion during endocytosis, thus triggering the overall conformational change in TRPML1. Based on the structural comparisons, we propose TRPML1 is regulated by pH, Ca2+, and phosphoinositides in a combined manner so as to accommodate the dynamic endocytosis process.

  6. Estudo do efeito da incorporação de plastificante de fonte renovável em compostos de PVC

    Directory of Open Access Journals (Sweden)

    Derval dos Santos Rosa

    2013-01-01

    Full Text Available O acúmulo de resíduos poliméricos tem crescido nos últimos anos e os subsolos dos lixões já estão saturados com resíduos plásticos. Isso possivelmente se deve à elevada produção de resíduos sólidos de plásticos (cerca de 25 mil t/dia em 2008, segundo a Lei de Resíduos. Em vista disso, este trabalho apresenta uma análise comparativa do estudo do potencial de (biodegradação de compostos de PVC utilizando um plastificante de origem vegetal renovável (PFR com estrutura de éster de milho com massa molar 296,5 g.mol- 1 e dois plastificantes sintéticos, ftalato de dioctila (DOP e adipato de dioctila (DOA, buscando inovar na obtenção de um composto de PVC com um plastificante de fonte renovável. Os resultados de FTIR evidenciaram maior interação do PFR com a resina de PVC para o teor de 30 phr, e para o teor de 40 phr observou-se que os plastificantes DOP e PFR apresentaram os mesmos valores de variação da banda atática. Já os ensaios mecânicos reforçaram os resultados de FTIR com maior compatibilidade para as composições contendo PFR. Uma maior estabilidade térmica no composto de PVC foi observada por TGA para a amostra contendo 40 phr de DOA, o que foi confirmado por RMN, e para a amostra com PFR nos teores de 30 e 40 phr. Por fim, uma perda significativa de massa foi observada nos ensaios de resistência à extração por solvente para os compostos contendo DOA, sendo que a amostra com 30 phr mostrou menor valor de extração. A biodegradação em solo simulado mostrou maior perda de massa para as amostras plastificadas com DOA (40phr e PFR (40phr. Isso indica que é viável usar plastificante de fonte renovável nos compostos de PVC, devido à compatibilidade deste com esta resina.

  7. 2,2,2-Trifluoroethyl methacrylate-graft-natural rubber: Synthesis and application as compatibilizer in natural rubber/fluoroelastomer blends

    Energy Technology Data Exchange (ETDEWEB)

    Hinchiranan, N., E-mail: nhn78@hotmail.com [Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand); Wannako, P. [Program in Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand); Paosawatyanyong, B. [Department of Physics, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand); Prasassarakich, P. [Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330 (Thailand)

    2013-05-15

    The incompatibility and immiscibility of natural rubber (NR)/fluoroelastomers (FKM) blends were improved by incorporation of a graft copolymer synthesized from the free radical graft copolymerization of 2,2,2-trifluoroethyl methacrylate (TFEM) onto NR initiated by benzoyl peroxide via a melt-mixing process. The grafting properties were investigated as functions of the initiator and monomer concentrations, reaction temperature and time. At the optimal conditions, the obtained graft NR (GNR) purified by Soxhlet extraction contained a maximum grafting efficiency of 1.34% with 49.1% monomer conversion. The structure of the purified GNR was analyzed using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and nuclear magnetic resonance spectroscopy ({sup 1}H NMR and {sup 19}F NMR). The gross GNR was then applied as the compatibilizer for NR/FKM vulcanizates (20/80 (w/w)) cured by peroxide vulcanization. The addition of GNR at 15 parts per hundred of rubber (phr) gave a vulcanizate with the highest tensile strength (9.93 MPa), some 5.31-fold higher than that of the incompatibilized one (1.87 MPa). This is likely to be due to the higher degree of homogeneity of the constituent phases in the GNR-compatibilized blends, as observed by scanning electron microscopy (SEM). The GNR-compatibilized NR/FKM vulcanizates were also resistant to gasohol E85 (85% (w/w) of ethanol). Highlights: ► 2,2,2-Trifluoroethyl methacrylate could be grafted on NR via melt-mixing process. ► Effect of grafting parameters on grafting efficiency and gel content was observed. ► NR/FKM containing graft NR had higher tensile strength than uncompatibilized ones. ► The compatibilized NR/FKM vulcanizates had higher gasohol-swelling resistance.

  8. African Zoology - Vol 29, No 2 (1994)

    African Journals Online (AJOL)

    Population biology of house mice (Mus musculus L.) on sub-Antarctic Marion Island · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. D.C. Matthewson, R.J. van Aarde, J.D. Skinner, 99-106 ...

  9. Arabidopsis CDS blastp result: AK105677 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105677 001-201-B01 At2g39090.1 tetratricopeptide repeat (TPR)-containing protein low similarity to prediab...etic NOD sera-reactive autoantigen [Mus musculus] GI:6670773, anaphase-promoting co

  10. Arabidopsis CDS blastp result: AK119376 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119376 001-132-A09 At2g39090.1 tetratricopeptide repeat (TPR)-containing protein low similarity to prediab...etic NOD sera-reactive autoantigen [Mus musculus] GI:6670773, anaphase-promoting co

  11. Arabidopsis CDS blastp result: AK101105 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK101105 J033025D11 At2g39090.1 tetratricopeptide repeat (TPR)-containing protein low similarity to prediabe...tic NOD sera-reactive autoantigen [Mus musculus] GI:6670773, anaphase-promoting com

  12. GenBank blastx search result: AK061938 [KOME

    Lifescience Database Archive (English)

    Full Text Available ative exons 18 and 18a, and alternative splice products, complete cds.|ROD ROD 1e-24 +2 ... ...AK061938 001-042-D07 AF071946.1 Mus musculus protein tyrosine kinase Tec (Tec) gene, exons 16 and 17, altern

  13. Experiment list: SRX286380 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 2,497 GSM1146459: AR Cast1a [prostate, castrated+vehicle]; Mus musculus; ChIP-Seq source_name=AR_Cast1a || s...train=wild type ICR || tissue=prostate || age=8-12 weeks old || treatment=castrated+vehicle || chip antibody

  14. Experiment list: SRX286386 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 60.2,17474 GSM1146465: FoxA1 Cast [prostate, castrated+vehicle]; Mus musculus; ChIP-Seq source_name=FoxA1_Ca...st || strain=wild type ICR || tissue=prostate || age=8-12 weeks old || treatment=castrated+vehicle || chip a

  15. The Effect of Calcusol™ to the Plasma Free Radical and Serum Creatinin in Mus Musculus Nephrolithiasis Model

    Directory of Open Access Journals (Sweden)

    A’liyatur Rosyidah

    2013-09-01

    Full Text Available Calcusol™ is a traditional medicine (jamu made from Tempuyung (Sonchus arvensis extract and is usually used for cure kidney stone disease. However, there has not been any studies which investigate the its mechanism. The aim of this study is to know the effect of Calcusol™ to the plasma free radical and serum creatinin of Mus musculus model for renal calcium-oxalate accumulation. This study is carried out by administration of Porang (Amorphophallus muelleri tuber flour for 3 months with the doses 6 mg/100g BW to induce renal calcium oxalate accumulation and Calcusol™ treatment for 7 days with the doses of 3.3mg/g BW. Group I was used as a control group. Group II was only given porang every day for 3 months. Group III was only given Calcusol™ for 7 days. Group IV were given porang for 3 months then given Calcusol™ for 7 days. Group V were given porang and Calcusol™ simultaneously for 3 months. Porang and CalcusolTM is administrated orally. Blood was collected from the tail of the animal for serum creatinin test and plasma free radical test using TBARS method. The data was analyzed using ANOVA followed by Tukey HSD to compare the means employing SPSS 16.0 for windows. The result of the research shows that the treatment Calcusol™ on mice model for renal calcium oxalate accumulation. The content of MDA at group I, group II, group III, group IV and group V, respectivelyis 0.81±0.5 mg/ml; 2.63±0.8 mg/ml; 0.56±0.5 mg/ml; 2.09±0.9 mg/ml and 0.17±0.17 mg/ml. The content of serum creatinine are 0.15±0.04 mg/dL; 0.13±0.03 mg/dL; 0.12±0.08 mg/dL; 0.11±0.016 mg/dL and 0.14±0.015 mg/dL at group I, group II, group III, Group IV, and group V respectively. This indicates that Calcusol™ decreases plasma free radical production during renal stone formation, while serum creatinin reduced but not significantly changed. Calcusol™ has an effect to decrease free radical during renalstone formation inmice model for calcium oxalate accumulation

  16. Experiment list: SRX286407 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 19696667,94.8,45.4,7089 GSM1146486: AP2a Cast1a [caput epididymis, castrated+vehicle]; Mus musculus; ChIP-Se...q source_name=AP2a_Cast1a || strain=wild type ICR || tissue=caput epididymis || age=8-12 weeks old || treatment=castrated+vehicle

  17. Experiment list: SRX286408 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 15944359,94.3,40.0,7036 GSM1146487: AP2a Cast1b [caput epididymis, castrated+vehicle]; Mus musculus; ChIP-Se...q source_name=AP2a_Cast1b || strain=wild type ICR || tissue=caput epididymis || age=8-12 weeks old || treatment=castrated+vehicle

  18. Dicty_cDB: SLJ215 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .7_1.5_KB Zea mays genomic clone ZMMBMa0453M17, genomic survey sequence. 42 4.5 1 BF012148 |BF012148.1 us38h03.y1 Soares_NMEBA_branch...ial_arch Mus musculus cDNA clone IMAGE:3169397 5' similar to TR:Q9QYU2 Q9QYU2 ELONG

  19. Carcass traits, blood serum and meat lipid fractions in Polish Landrace pigs differing in RYR1 genotype

    OpenAIRE

    Janik A.; Barowicz T.; Pieszka M.; Migdai W.

    2005-01-01

    The aim of study was to investigate the effect of RYR1 genotypes on carcass traits and lipid fractions in blood serum and musculus longissimus dorsi of Polish Landrace pigs. The fatteners with RYR1CRYR1 genotype had lower level of triglycerides, total cholesterol, HDL- and LDL cholesterol in blood serum than individuals from RYR1CRYR1C group. The same group of animals had lower amount of intramuscular fat and cholesterol in comparison to homozygotes RYR1CRYR1C. The intramuscular fat of hetero...

  20. Preparation of High Density Polyethylene/Waste Polyurethane Blends Compatibilized with Polyethylene-Graft-Maleic Anhydride by Radiation

    Directory of Open Access Journals (Sweden)

    Jong-Seok Park

    2015-04-01

    Full Text Available Polyurethane (PU is a very popular polymer that is used in a variety of applications due to its good mechanical, thermal, and chemical properties. However, PU recycling has received significant attention due to environmental issues. In this study, we developed a recycling method for waste PU that utilizes the radiation grafting technique. Grafting of waste PU was carried out using a radiation technique with polyethylene-graft-maleic anhydride (PE-g-MA. The PE-g-MA-grafted PU/high density polyethylene (HDPE composite was prepared by melt-blending at various concentrations (0–10 phr of PE-g-MA-grafted PU. The composites were characterized using fourier transform infrared spectroscopy (FT-IR, and their surface morphology and thermal/mechanical properties are reported. For 1 phr PU, the PU could be easily introduced to the HDPE during the melt processing in the blender after the radiation-induced grafting of PU with PE-g-MA. PE-g-MA was easily reacted with PU according to the increasing radiation dose and was located at the interface between the PU and the HDPE during the melt processing in the blender, which improved the interfacial interactions and the mechanical properties of the resultant composites. However, the elongation at break for a PU content >2 phr was drastically decreased.

  1. Effect of dynamic crosslinking on phase morphology and mechanical properties of polyamide 6,12/ethylene vinyl acetate copolymer blends

    Directory of Open Access Journals (Sweden)

    Fabrício Bondan

    2015-03-01

    Full Text Available The dynamic crosslinking of polyamide 6,12 and ethylene vinyl acetate (PA6,12/EVA blends in the mixing chamber of a torque rheometer was investigated. EVA was selectively crosslinked within the PA6,12 phase through free radical reactions using dycumil peroxide. The degree of EVA crosslinking in the PA12,6/EVA materials was estimated based on the gel content (insoluble EVA fraction. The PA6,12/EVA phase morphology was investigated by scanning electron microscopy. The mechanical properties were investigated by determining the tensile strength and hardness. The half-life time ( for homolytic scission of the dcumil peroxide (DCP was ~6s, and this time is longer than the dispersion time of the DCP in the blends. The addition of DCP resulted in increased torque values due to specific crosslinking in the EVA phase. For the pure EVA and its blends with PA6,12 the stabilized torque values increased proportionally with the amount of DCP in the system, due to a higher degree of crosslinking of the elastomeric phase. The gel content of the dynamically crosslinked blends increased with the amount of DCP incorporated until 4 phr. At 1 phr the gel content value was 2.6wt.%, while at 4 phr it was 17wt.%. For the polymer blend with 8 phr of DCP a lubricating effect contributed to reducing the gel content. The dynamically crosslinked blends, regardless of the amount of DCP added, showed a reduction in the mechanical properties, which is related to the morphological features of the system due to the low mechanical fragmentation during melt processing.

  2. Trial production of low protein irradiated natural rubber latex by low energy electron beam in pilot scale

    International Nuclear Information System (INIS)

    Utama, Marga; Yoshii, F.; Kume, T.

    2006-01-01

    Three importance factors for producing low protein by low energy electron beam (250 keV/10 mA) irradiation in pilot scale (20 liters per bath) with 1,9-nonediol diacrylate (NDA) namely: maturation time of natural rubber latex before irradiation, treatment of irradiated natural rubber latex (INRL) before and after centrifugation, and standard irradiation method has been carried out. The results showed that the optimum irradiation time for producing INRL with 5 phr (part hundred ratio of rubber) of NDA as sensitize agent, and with the rotation speed of agitation 210 rpm (rotation per minutes) was between 20-30 minutes. By using this condition tensile strength of the INRL film was 26 MPa. The maturation of natural rubber latex before irradiation is the key for driving the quality of INRL. Water extractable protein content of INRL after leaching in 1% ammonia solution for 30 minutes at room temperature was around 47 μ/g, and after adding with 1 phr of PVA (poly vinyl alcohol) or 0.1 phr CMC (carboxy methyl cellulose) the water extractable protein content decrease less than 6 μ/g. (author)

  3. First report of [i]Enterocytozoon[/i] bieneusi and [i]Encephalitozoon intestinalis[/i] infection of wild mice in Slovakia

    Directory of Open Access Journals (Sweden)

    Oľga Danišová

    2015-05-01

    Full Text Available Increased risk of zoonotic transmission of the potential human pathogenic species [i]Enterocytozoon bieneusi[/i], [i]Encephalitozoon intestinalis[/i] and [i]Encephalitozoon cuniculi [/i]was detected in wild immunocompetent mice (Mus musculus musculus; n=280. Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, [i]E. bieneusi[/i] was detected in 3 samples (1.07 %, [i]E. cuniculi [/i]in 1 sample (0.35 % and [i]E. intestinalis[/i] in 1 sample (0.35 %. The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.

  4. Mitochondrial DNA in the hybrid zone between Mus musculus musculus and Mus musculus domesticus: a comparison of two transects

    Czech Academy of Sciences Publication Activity Database

    Božíková, Eva; Munclinger, P.; Teeter, K. C.; Tucker, P. K.; Macholán, Miloš; Piálek, Jaroslav

    2005-01-01

    Roč. 84, č. 3 (2005), s. 363-378 ISSN 0024-4066. [The genus Mus as a model for evolutionary studies - a symposium in honour of Louis Thaler. Brno, 28.07.2003-30.07.2003] R&D Projects: GA ČR GA206/03/0205; GA ČR GA206/01/0989 Institutional research plan: CEZ:AV0Z6093917 Keywords : allozyme * gene flow * house mouse Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.261, year: 2005

  5. A framework and approach for assessing the value of personal health records (PHRs).

    Science.gov (United States)

    Johnston, Douglas; Kaelber, David; Pan, Eric C; Bu, Davis; Shah, Sapna; Hook, Julie M; Middleton, Blackford

    2007-10-11

    Personal Health Records (PHRs) are a rapidly expanding area of medical informatics due to the belief that they may improve health care delivery and control costs of care. The PHRs in use or in development today support a myriad of different functions, and consequently offer different value propositions. A comprehensive value analysis of PHRs has never been conducted; such analysis is needed to identify those PHR functions that yield the greatest value to PHR stakeholders. Here we present a framework that could serve as a foundation for determining the value of PHR functions and thereby help optimize PHR development. While the value framework is specific to the domain of PHRs, the authors have successfully applied the associated evaluation methodology in assessing other health care information technologies.

  6. NCBI nr-aa BLAST: CBRC-STRI-01-2626 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-STRI-01-2626 sp|Q8VBS7|VN1A6_MOUSE RecName: Full=Vomeronasal type-1 receptor A...6; AltName: Full=Vomeronasal type-1 receptor A3; AltName: Full=Vomeronasal type-1 receptor A1; AltName: Full=Vomeronasa...l type-1 receptor A10 dbj|BAB79209.1| vomeronasal receptor 1 A1 [Mus musculus] dbj|BAB79210.1| vomeronasa...l receptor 1 A3 [Mus musculus] gb|AAK98772.1| vomeronasal receptor V1RA10

  7. Penelitian “rubber blend” antara karet alam rss dengan karet sintesis jsr 0061 untuk sol ringan sesuai sni 12-0778-1989

    Directory of Open Access Journals (Sweden)

    Penny Setyowati

    1997-12-01

    Full Text Available The objective of this research is to find composision of rubber blend of RSS and JSR 0061 and Carbon black for preparation light rubber sole compound and stabile on high process temperature that give the best fisical properties and the best sole product at moulding vulcanization trial. The best rubber compound to be reached at the compound formulation R III with composision of RSS 90 phr, JSR 0061 10 phr dan carbon black 40 phr.

  8. Multiple loci affecting photoreactivation in Escherichia coli

    International Nuclear Information System (INIS)

    Sutherland, B.M.; Hausrath, S.G.

    1979-01-01

    Sutherland et al. mapped a phr gene in Escherichia coli at 17 min and found that induction of an E. coli stain lysogenic for a lambda phage carrying this gene increased photoreactivating enzyme levels 2,000-fold. Recently, Smith and Youngs and Sancar and Rupert located a phr gene at 15.9 min. We have therefore investigated the properties of photoreactivating enzyme and cellular photoreactivation in cells containing deletions of the gene at 17 min. Cells with this deletion photoreactivated ultraviolet-induced killing at a rate 20% of normal; they also contained approximately 20% of the normal photoreactivating enzyme level. The residual enzyme in these cells was characterized to determine whether the reduced cellular photoreactivation rate and photoreactivating enzyme levels resulted from reduced numbers of normal enzymes or from an altered enzyme. Photoreactivating enzymes from strains carrying a deletion of the region at 17 min has an apparent K/sub m/ about two- to threefold higher than normal enzyme and showed markedly increased heat lability. The gene at 17 min thus contains information determining the function of the E. coli photoreactivating enzyme rather than the quantity of the enzyme. It is proposed that the gene at 17 min be termed phrA and that located at 15.9 min be termed phrB

  9. Radiation vulcanization of natural rubber latex using irradiation n-butyl acrylate aqueous emulsion as sensitizer

    International Nuclear Information System (INIS)

    Vo Van Thien; Nguyen Quoc Hien; Keizo Makuuchi; Fumio Yoshii

    2000-01-01

    Natural rubber latex was vulcanized by gamma radiation with n-butyl acrylate aqueous emulsion irradiated at dose of 1.5 kGy as sensitizer. The total solid content of latex increases on the irradiation dose. The viscosity of latex on the standing time was investigated and became stable after one month of storage. The gel content of latex films increasing with irradiation dose and attained more than 94% at dose of 10 kGy. Tensile strength of films reached the values of 31MPa; 30 Mpa and 25 Mpa at vulcanization doses of 20 kGy, 15 kGy and 8 kGy for the concentrations of sensitizer 7 phr, 9 phr and 13 phr respectively. Elongation at break decreases as increasing dose. Tear strength of rubber films was from 30-40 N/mm. The tackiness of latex films decreases and smell of vulcanized latex is almost negligible. (author)

  10. Chromatin preferences of the perichromosomal layer constituent pKi-67.

    Science.gov (United States)

    Traut, Walther; Endl, Elmar; Garagna, Silvia; Scholzen, Thomas; Schwinger, Eberhard; Gerdes, Johannes; Winking, Heinz

    2002-01-01

    The proliferation-associated nuclear protein pKi-67 relocates from the nucleolus to the chromosome surface during the G2/M transition of the cell cycle and contributes to the formation of the 'perichromosomal layer'. We investigated the in-vivo binding preferences of pKi-67 for various chromatin blocks of the mitotic chromosomes from the human and two mouse species, Mus musculus and M. caroli. All chromosomes were decorated with pKi-67 but displayed a gap of pKi-67 decoration in the centromere and NOR regions. pKi-67 distribution in a rearranged mouse chromosome showed that the formation of the centromeric gap was controlled by the specific chromatin in that region. While most chromatin served as a substrate for direct or indirect binding of pKi-67, we identified three types of chromatin that bound less or no pKi-67. These were: (1) the centromeric heterochromatin defined by the alpha satellite DNA in the human, by the mouse minor satellite in M. musculus and the 60- and 79-bp satellites in M. caroli; (2) the pericentromeric heterochromatin in M. musculus defined by the mouse major satellite, and (3) NORs in the human and in M. musculus defined by rDNA repeats. In contrast, the conspicuous blocks of pericentromeric heterochromatin in human chromosomes 1, 9 and 16 containing the 5-bp satellite showed intense pKi-67 decoration. The centromeric gap may have a biological significance for the proper attachment of the chromosomes to the mitotic spindle. In this context, our results suggest a new role for centromeric heterochromatin: the control of the centromeric gap in the perichromosomal layer.

  11. Dissecting the genetic architecture of F1 hybrid sterility in house mice.

    Science.gov (United States)

    Dzur-Gejdosova, Maria; Simecek, Petr; Gregorova, Sona; Bhattacharyya, Tanmoy; Forejt, Jiri

    2012-11-01

    Hybrid sterility as a postzygotic reproductive isolation mechanism has been studied for over 80 years, yet the first identifications of hybrid sterility genes in Drosophila and mouse are quite recent. To study the genetic architecture of F(1) hybrid sterility between young subspecies of house mouse Mus m. domesticus and M. m. musculus, we conducted QTL analysis of a backcross between inbred strains representing these two subspecies and probed the role of individual chromosomes in hybrid sterility using the intersubspecific chromosome substitution strains. We provide direct evidence that the asymmetry in male infertility between reciprocal crosses is conferred by the middle region of M. m. musculus Chr X, thus excluding other potential candidates such as Y, imprinted genes, and mitochondrial DNA. QTL analysis identified strong hybrid sterility loci on Chr 17 and Chr X and predicted a set of interchangeable autosomal loci, a subset of which is sufficient to activate the Dobzhansky-Muller incompatibility of the strong loci. Overall, our results indicate the oligogenic nature of F(1) hybrid sterility, which should be amenable to reconstruction by proper combination of chromosome substitution strains. Such a prefabricated model system should help to uncover the gene networks and molecular mechanisms underlying hybrid sterility. © 2012 The Author(s). Evolution© 2012 The Society for the Study of Evolution.

  12. Experiment list: SRX1056357 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available || cell type=ES cells || treated with=2.5 µM tamoxifen (Tam) || chip antibody=none http://dbarchive.bioscien...tiate into specialized cells. 35337197,98.9,19.2,224 GSM1708671: input DNA +Tam R...2; Mus musculus; ChIP-Seq source_name=input DNA_+Tam || strain=J1 || genotype/variation=inducible SetDB1 KO

  13. Personal health records as portal to the electronic medical record.

    Science.gov (United States)

    Cahill, Jennifer E; Gilbert, Mark R; Armstrong, Terri S

    2014-03-01

    This topic review discusses the evolving clinical challenges associated with the implementation of electronic personal health records (PHR) that are fully integrated with electronic medical records (EMR). The benefits of facilitating patient access to the EMR through web-based, PHR-portals may be substantial; foremost is the potential to enhance the flow of information between patient and healthcare practitioner. The benefits of improved communication and transparency of care are presumed to be a reduction in clinical errors, increased quality of care, better patient-management of disease, and better disease and symptom comprehension. Yet PHR databases allow patients open access to newly-acquired clinical data without the benefit of concurrent expert clinical interpretation, and therefore may create the potential for greater patient distress and uncertainty. With specific attention to neuro-oncology patients, this review focuses on the developing conflicts and consequences associated with the use of a PHR that parallels data acquisition of the EMR in real-time. We conclude with a discussion of recommendations for implementing fully-integrated PHR for neuro-oncology patients.

  14. Evidencias empíricas de regularidades estadísticas y leyes de potencia en los genomas de Arabidopsis thaliana, Oriza sativa y Mus musculus Empirical evidences of statistical regularities and power laws in the genomes of Arabidopsis thaliana, Oriza sativa and Mus musculus

    Directory of Open Access Journals (Sweden)

    Martha I Almanza P.

    2010-07-01

    Full Text Available La masiva cantidad de datos biológicos provenientes de las disciplinas "ómicas" y su aprovechamiento en el mejoramiento genético vegetal requiere de nuevos abordajes teóricos y estadísticos que describan de forma satisfactoria principios generales en los genomas. El total de secuencias de los genes de los genomas vegetales de Arabidopsis thaliana y Oriza sativa y del genoma animal Mus musculus fueron extraídas y depuradas de la base de datos pública del Genebank mediante el diseño de algoritmos en lenguaje de programación Python. Se analizaron las distribuciones de las variables frecuencia de uso y tamaño de los genes, exones e intrones por cromosoma y entre genomas. Los resultados señalaron que las variables presentan patrones de comportamiento no lineales en forma de ley de potencia que difieren estadísticamente entre los genomas pero no entre los cromosomas de un mismo genoma. Además, el análisis aportó evidencias respecto al tamaño promedio constante de las secuencias de exones y de los genes simples por cromosoma y entre genomas. Los hallazgos sugieren: primero, que el genoma se auto-organiza de la misma manera en los cromosomas independientemente del tamaño o número de genes que estos contengan, y, segundo, que tanto los cromosomas como sus elementos constituyentes: genes, exones e intrones han evolucionado conjuntamente. El estudio señala que las leyes de potencia cumplen un papel amortiguador en las leyes de variación biológica y proporcionan medidas cuantitativas de la organización de las secuencias de ADN que definen la identidad de un genoma. La regularidad estadística de estas medidas genéticas tiene potenciales aplicaciones en el incremento del valor predictivo de los actuales modelos de mejoramiento genético vegetal.The huge quantity of biological data arising from the omics disciplines and their benefit in plant breeding require of new theoretical and statistical approaches in order to get a satisfactory

  15. AcEST: DK950437 [AcEST

    Lifescience Database Archive (English)

    Full Text Available n Hook homolog 1 OS=Mus musculus GN=... 45 4e-04 sp|Q9UJC3|HOOK1_HUMAN Protein Hook homolog 1 OS=Homo sapien... 483 IVELESQVDSLSARVAELNTERMSSQQ 563 I EL+ Q++ ++ EL TE+ S++ Sbjct: 489 IEELQEQLEQKHRKMNELETEQRLSKE 515 >sp|Q9Y2K

  16. The Effect of Concentration of P-phenylenediamine Antioxidant on the Acrylonitrile-Butadiene Rubber Seals under High Gamma Irradiation

    International Nuclear Information System (INIS)

    Hegazi, E.M.; Abd El-megeed, A.A.

    2016-01-01

    Acrylonitrile- butadiene rubber (NBR) seals are one of the classified seals used in nuclear facilities. But at high irradiation doses the physical and mechanical properties of NBR are adversely affected due to the degradation induced by radiation and hence affect the sealing performance reducing their service life. The present work is focused on studying the effect of concentration of N-(1, 3-dimethylbutyl)-N’-phenyl-p-phenylene diamine (6PPD) on the physical and mechanical properties of the NBR rubber at high doses of γ-irradiation up to 2 MGy. The physical properties, mechanical properties, hardness, and abrasion of the NBR rubber under γ-radiation were investigated. The optimum amount of 6PPD required to resist deterioration is also estimated. The results showed a remarkable increase in the physical and mechanical properties as the concentration of 6PPD was increased from 1 phr (part per hundred) to 3 phr in NBR samples

  17. AcEST: BP912733 [AcEST

    Lifescience Database Archive (English)

    Full Text Available C and casein kinase substrate in neurons protein 1 OS=Mus musculus GN=Pacsin1 PE...se substrate in neurons protein 1 OS=Rattus norvegicus GN=Pacsin1 PE=1 SV=1 Length = 441 Score = 32.3 bits (...e in neurons protein 1 OS=Pongo abelii GN=Pacsin1 PE=2 SV=1 Length = 444 Score = 32.0 bits (71), Expect = 2.

  18. Metabolism of 1-fluoro-1,1,2-trichloroethane, 1,2-dichloro-1,1-difluoroethane, and 1,1,1-trifluoro-2-chloroethane.

    Science.gov (United States)

    Yin, H; Jones, J P; Anders, M W

    1995-03-01

    1-Fluoro-1,1,2-trichloroethane (HCFC-131a), 1,2-dichloro-1,1-difluoroethane (HCFC-132b), and 1,1,1-trifluoro-2-chloroethane (HCFC-133a) were chosen as models for comparative metabolism studies on 1,1,1,2-tetrahaloethanes, which are under consideration as replacements for ozone-depleting chlorofluorocarbons (CFCs). Male Fischer 344 rats were given 10 mmol/kg ip HCFC-131a or HCFC-132b or exposed by inhalation to 1% HCFC-133a for 2 h. Urine collected in the first 24 h after exposure was analyzed by 19F NMR and GC/MS and with a fluoride-selective ion electrode for the formation of fluorine-containing metabolites. Metabolites of HCFC-131a included 2,2-dichloro-2-fluoroethyl glucuronide, 2,2-dichloro-2-fluoroethyl sulfate, dichlorofluoroacetic acid, and inorganic fluoride. Metabolites of HCFC-132b were characterized as 2-chloro-2,2-difluoroethyl glucuronide, 2-chloro-2,2-difluoroethyl sulfate, chlorodifluoroacetic acid, chlorodifluoroacetaldehyde hydrate, chlorodifluoroacetaldehyde-urea adduct, and inorganic fluoride. HCFC-133a was metabolized to 2,2,2-trifluoroethyl glucuronide, trifluoroacetic acid, trifluoroacetaldehyde hydrate, trifluoroacetaldehyde-urea adduct, inorganic fluoride, and a minor, unidentified metabolite. With HCFC-131a and HCFC-132b, glucuronide conjugates of 2,2,2-trihaloethanols were the major urinary metabolites, whereas with HCFC-133a, a trifluoroacetaldehyde-urea adduct was the major urinary metabolite. Analysis of metabolite distribution in vivo indicated that aldehydic metabolites increased as fluorine substitution increased in the order HCFC-131a < HCFC-132b < HCFC-133a. With NADPH-fortified rat liver microsomes, HCFC-133a and HCFC-132b were biotransformed to trifluoroacetaldehyde and chlorodifluoroacetaldehyde, respectively, whereas HCFC-131a was converted to dichlorofluoroacetic acid. No covalently bound metabolites were detected by 19F NMR spectroscopy.(ABSTRACT TRUNCATED AT 250 WORDS)

  19. Radiation shielding material characterization by non-destructive neutron radiography technique

    International Nuclear Information System (INIS)

    Hafizal Yazid; Azali Muhammad; Abdul Aziz Mohamed; Rafhayudi Jamro; Hishamuddin Husain

    2007-01-01

    Shielding property of boronated rubber was characterized easily by the use of neutron radiography technique. For 10 phr of boron carbide in the natural rubber composite, the ability to completely shield against neutron was found to have 8mm thickness and above for the neutron flux of 1.04 x 10 5 n/cm 2 s (author)

  20. Penggunaan Karet Alam pada Pembuatan Apron untuk Mesin Pemintalan

    OpenAIRE

    Luftinor, Luftinor

    2010-01-01

    The Research aims to increase the use of natural rubber in the textile industry especially is ring spinning machine, the research done by varying the amount of apron manufacture of natural rubber (SIR 20), synthetic rubber (SBR), and filler materials (carbon black) in 3 levels of each treatment each 25 phr, 50 phr and 75 phr, obtained nine kinds of rubber compounds. The nine kinds of vulcanized rubber compounds to obtain finished goods apron, next physics test. Physical properties test result...

  1. Dicty_cDB: VHE271 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available -21 (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL157766 |pid:none) 105 3e-21 BC171956_1( BC171956 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 103 1e-20 BC138482_1( BC138482 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 103 1e-20 (Q9JLC8) RecName: Full=Sacsin; 103 1e-20 AB006708_10( AB006

  2. NCBI nr-aa BLAST: CBRC-XTRO-01-3489 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-3489 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 1e-151 78% ...

  3. NCBI nr-aa BLAST: CBRC-OLAT-26-0036 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-26-0036 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 1e-55 36% ...

  4. Genetics and evolution of hybrid male sterility in house mice.

    Science.gov (United States)

    White, Michael A; Stubbings, Maria; Dumont, Beth L; Payseur, Bret A

    2012-07-01

    Comparative genetic mapping provides insights into the evolution of the reproductive barriers that separate closely related species. This approach has been used to document the accumulation of reproductive incompatibilities over time, but has only been applied to a few taxa. House mice offer a powerful system to reconstruct the evolution of reproductive isolation between multiple subspecies pairs. However, studies of the primary reproductive barrier in house mice-hybrid male sterility-have been restricted to a single subspecies pair: Mus musculus musculus and Mus musculus domesticus. To provide a more complete characterization of reproductive isolation in house mice, we conducted an F(2) intercross between wild-derived inbred strains from Mus musculus castaneus and M. m. domesticus. We identified autosomal and X-linked QTL associated with a range of hybrid male sterility phenotypes, including testis weight, sperm density, and sperm morphology. The pseudoautosomal region (PAR) was strongly associated with hybrid sterility phenotypes when heterozygous. We compared QTL found in this cross with QTL identified in a previous F(2) intercross between M. m. musculus and M. m. domesticus and found three shared autosomal QTL. Most QTL were not shared, demonstrating that the genetic basis of hybrid male sterility largely differs between these closely related subspecies pairs. These results lay the groundwork for identifying genes responsible for the early stages of speciation in house mice.

  5. Experiment list: SRX483614 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 32 GSM1342522: iPSC SNEL1; Mus musculus; ChIP-Seq source_name=reprogramming from MEF cell || cell line=iPSC ...SNEL1 || passage=15-20 || reprogramming factors=Sall4, Nanog, Esrrb and Lin28 || antibody=H2A.X (Xiao lab) h

  6. Arang aktif sekam padi sebagai bahan pengisi kompon sol karet

    Directory of Open Access Journals (Sweden)

    Arum Yuniari

    2003-06-01

    Full Text Available Abstract The aim of this research was to investigate the properties of activated carbon from rice husk filler in their application on vulcanized rubber soles, and to determine its suitability for rubber compound, the properties of activated carbon from rice husk filler in their application on vulcanized rubber soles, and to determine its suitability for rubber compound, the properties of activated carbon made from rice husk was investigated by comparing with cabon black N 330. Activated carbon made from rice husk was made by carbonization process at temperature of 4500C for 1 hour and activation process with NaCL 4% for 24 hours followed by pyrolysis at temperature 5000C for 1 hour. Filler was milled and sieved by 400 mesh siefter. The research showed that using activated carbon in the amount 20 phr and carbon black N330 40 phr. Fulfill the requirement at SNI : 12-0172-1987 : Canvas shoes sole for general purpose, where as tensile strength 11,24 N/mm2, elongation at break : 699%, tear strength 2,97 N/mm2, hardness : 65,7 shore A, density 1,23 gram/cm2, permanent set 0,10%, abrasion 0,72 mm3/ kgm and flexing at 250 kcs no crack.

  7. RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans.

    Science.gov (United States)

    Grill, Brock; Chen, Lizhen; Tulgren, Erik D; Baker, Scott T; Bienvenut, Willy; Anderson, Matthew; Quadroni, Manfredo; Jin, Yishi; Garner, Craig C

    2012-02-22

    Previous studies in Caenorhabditis elegans showed that RPM-1 (Regulator of Presynaptic Morphology-1) regulates axon termination and synapse formation. To understand the mechanism of how rpm-1 functions, we have used mass spectrometry to identify RPM-1 binding proteins, and have identified RAE-1 (RNA Export protein-1) as an evolutionarily conserved binding partner. We define a RAE-1 binding region in RPM-1, and show that this binding interaction is conserved and also occurs between Rae1 and the human ortholog of RPM-1 called Pam (protein associated with Myc). rae-1 loss of function causes similar axon and synapse defects, and synergizes genetically with two other RPM-1 binding proteins, GLO-4 and FSN-1. Further, we show that RAE-1 colocalizes with RPM-1 in neurons, and that rae-1 functions downstream of rpm-1. These studies establish a novel postmitotic function for rae-1 in neuronal development.

  8. Micro-evolutionary divergence patterns of mandible shapes in wild house mouse (Mus musculus populations

    Directory of Open Access Journals (Sweden)

    Tautz Diethard

    2011-10-01

    Full Text Available Abstract Background Insights into the micro-evolutionary patterns of morphological traits require an assessment of the natural variation of the trait within and between populations and closely related species. The mouse mandible is a particularly suitable morphological trait for such an analysis, since it has long been used as a model to study the quantitative genetics of shape. In addition, many distinct populations, sub-species and closely related species are known for the house mouse. However, morphological comparisons among wild caught animals require an assessment in how far environmental and technical factors could interfere with the shape change measurements. Results Using geometric morphometrics, we have surveyed mandible shapes in 15 natural populations of the genus Mus, with a focus on the subspecies Mus musculus domesticus. In parallel we have carefully assessed possibly confounding technical and biological factors. We find that there are distinct differences on average between populations, subspecies and species, but these differences are smaller than differences between individuals within populations. Populations from summer-dry regions, although more ancestral, are less distinct from each other than are populations from the more recently colonized northern areas. Populations with especially distinct shapes occur in an area of sympatry of M. m. domesticus and M. spretus and on recently colonized sub-antarctic islands. We have also studied a number of inbred strains to assess in how far their mandible shapes resemble those from the wild. We find that they fall indeed into the shape space of natural variation between individuals in populations. Conclusions Although mandible shapes in natural populations can be influenced by environmental variables, these influences are insufficient to explain the average extent of shape differences between populations, such that evolutionary processes must be invoked to explain this level of diversity

  9. Pengaruh Penggunaan Nr dan Nbr dengan Bahan Pengisi Silika terhadap Sifat Fisika Kompon Rol Karet Cpo

    OpenAIRE

    Prasetya, Hari Adi

    2010-01-01

    The research aimed to obtain the influence of natural rubber NR, synthetic rubber NBR and alumunium silikat (AISiO₃) as a filler, its physical properties and also to find out rubber compound the best for rubber mixture with variation of NR : NBR 50, 70 : 30, 90 : 10 hr and variation of aluminium silikat (ASiO₂) 45 phr, 50 phr, 55 phr. The result showed that the addition of rubber NR : NBR ratio and alumunium silikat (ASiO₂) had significant effect on the hardness, tensile strength, abration re...

  10. Unigene BLAST: CBRC-MMUS-03-0073 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-03-0073 gnl|UG|Mm#S8040853 Mus musculus opsin 1 (cone pigments), short-wave-sensitive (color blind...ness, tritan) (Opn1sw), mRNA /cds=p(66,1106) /gb=NM_007538 /gi=31543369 /ug=Mm.56987 /len=2521 2e-27 27% ...

  11. Experiment list: SRX897941 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 081,98.0,12.4,11292 GSM1624626: ChIP seq Chaf1a.166 Sox2 IP day3; Mus musculus; ChIP-Seq source_name=OKSM reprogramming... intermediates from Mouse Embryonic Fibroblasts || strain=Black6-129X1/SvJ || cell type=OKSM reprogramming

  12. Unigene BLAST: CBRC-MMUS-17-0171 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-17-0171 gnl|UG|Mm#S8040853 Mus musculus opsin 1 (cone pigments), short-wave-sensitive (color blind...ness, tritan) (Opn1sw), mRNA /cds=p(66,1106) /gb=NM_007538 /gi=31543369 /ug=Mm.56987 /len=2521 2e-22 22% ...

  13. Dicty_cDB: Contig-U01725-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available pid:none) Paramecium tetraurelia, complete g... 58 1e-07 G88391( G88391 )protein R06B10.5 [imported] - Caeno... Micromonas sp. RCC299 chromosome ... 37 0.32 ( Q92738 ) RecName: Full=USP6 N-terminal-like protein; AltName...domain family, m... 37 0.32 BC051184_1( BC051184 |pid:none) Mus musculus USP6 N-terminal like,... 37 0.42 AM...ar-... 37 0.42 (Q80XC3) RecName: Full=USP6 N-terminal-like protein; &AL845275_......a Japonica Group chromosome 3 clone OS... 36 0.72 3 ( EJ909987 ) 1093018533057 Global-Ocean-Sampli

  14. m2-ABKS: Attribute-Based Multi-Keyword Search over Encrypted Personal Health Records in Multi-Owner Setting.

    Science.gov (United States)

    Miao, Yinbin; Ma, Jianfeng; Liu, Ximeng; Wei, Fushan; Liu, Zhiquan; Wang, Xu An

    2016-11-01

    Online personal health record (PHR) is more inclined to shift data storage and search operations to cloud server so as to enjoy the elastic resources and lessen computational burden in cloud storage. As multiple patients' data is always stored in the cloud server simultaneously, it is a challenge to guarantee the confidentiality of PHR data and allow data users to search encrypted data in an efficient and privacy-preserving way. To this end, we design a secure cryptographic primitive called as attribute-based multi-keyword search over encrypted personal health records in multi-owner setting to support both fine-grained access control and multi-keyword search via Ciphertext-Policy Attribute-Based Encryption. Formal security analysis proves our scheme is selectively secure against chosen-keyword attack. As a further contribution, we conduct empirical experiments over real-world dataset to show its feasibility and practicality in a broad range of actual scenarios without incurring additional computational burden.

  15. The value of personal health records for chronic disease management: what do we know?

    Science.gov (United States)

    Tenforde, Mark; Jain, Anil; Hickner, John

    2011-05-01

    Electronic personal health records (PHRs) allow patients access to their medical records, self-management tools, and new avenues of communication with their health care providers. They will likely become a valuable component of the primary care Patient-centered Medical Home model. Primary care physicians, who manage the majority of chronic disease, will use PHRs to help patients manage their diabetes and other chronic diseases requiring continuity of care and enhanced information flow between patient and physician. In this brief report, we explore the evidence for the value of PHRs in chronic disease management. We used a comprehensive review of MEDLINE articles published in English between January 2000 and September 2010 on personal health records and related search terms. Few published articles have described PHR programs designed for use in chronic disease management or PHR adoption and attitudes in the context of chronic disease management. Only three prospective randomized trials have evaluated the benefit of PHR use in chronic disease management, all in diabetes care. These trials showed small improvements in some but not all diabetes care measures. All three trials involved additional interventions, making it difficult to determine the influence of patient PHR use in improved outcomes. The evidence remains sparse to support the value of PHR use for chronic disease management. With the current policy focus on meaningful use of electronic and personal health records, it is crucial to investigate and learn from new PHR products so as to maximize the clinical value of this tool.

  16. Experiment list: SRX977394 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 99.1,7.7,102329 GSM1648645: H3K4me1 ChipSeq day5; Mus musculus; ChIP-Seq source_name=reprogramming intermedi...ate after 5days of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA

  17. Experiment list: SRX977392 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 98.9,6.9,91916 GSM1648643: H3K4me1 ChipSeq day1; Mus musculus; ChIP-Seq source_name=reprogramming intermedia...te after 24hour of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA

  18. Preparation and mechanical properties of rubber composites reinforced with carbon nanohorns.

    Science.gov (United States)

    Isshiki, Tetsuya; Hashimoto, Mikiko; Morii, Masato; Ota, Yuki; Kaneda, Kazuo; Takahashi, Hidetaka; Yudasaka, Masako; Iijima, Sumio; Okino, Fujio

    2010-06-01

    Nitrile butadiene rubber (NBR) composites with single-wall carbon nanohorns (SWNHs, or simply NHs), hole-opened NHs (h-NHs), and carbon black (CB), the most commonly used nanocarbon rubber filler, were prepared, and their mechanical properties were compared. The NBR composites with h-NHs (NBR/h-NH) showed higher tensile strength than those with NHs (NBR/NH), and the tensile strength of NBR/h-NH or NBR/NH was much greater than those of the NBR composites with CB (NBR/CB). At 5 parts per hundred of rubber (phr), the tensile stresses at break of NBR/h-NH was about 1.8 times larger than those of NBR/CB, and the strain at the break, 1.2 times larger. Similarly, at 20 phr, both the tensile strength and strain at the break of NBR/h-NH were 1.4 times larger than those of NBR/CB. NBR/NH showed the highest hardness while having the smallest specific gravity. The present results indicate that NHs and h-NHs have much superior reinforcement effects to CB for NBR rubber matrix.

  19. A cross-cultural analysis of posthumous reproduction: The significance of the gender and margins-of-life perspectives

    Directory of Open Access Journals (Sweden)

    Yael Hashiloni-Dolev

    2017-06-01

    Full Text Available The scholarly discussion of posthumous reproduction (PHR focuses on informed consent and the welfare of the future child, for the most part overlooking cultural differences between societies. Based on a cross-cultural comparison of legal and regulatory documents, analysis of pivotal cases and study of scholarly and media discussions in Israel and Germany, this paper analyses the relevant ethical and policy issues, and questions how cultural differences shape the practice of PHR. The findings challenge the common classifications of PHR by highlighting the gender perspective and adding brain-dead pregnant women to the debate. Based on this study’s findings, four neglected cultural factors affecting social attitudes towards PHR are identified: (i the relationship between the pregnant woman and her future child; (ii what constitutes the beginning of life; (iii what constitutes dying; and (iv the social agent(s seeking to have the future child. The paper argues that PHR can be better understood by adding the gender and margins-of-life perspectives, and that future ethical and practical discussions of this issue could benefit from the criteria emerging from this cross-cultural analysis.

  20. Optimization of Mechanical, Dynamical and Thermal Properties of a High Performance Tread Compound for Radial Tires

    Directory of Open Access Journals (Sweden)

    Mir Hamid Reza Ghoreishy

    2013-06-01

    Full Text Available A high performance passenger tire tread compound was optimized for its mechanical, dynamical and thermal properties. A reference compound was based on a blend of SBR and BR, sulfur and other ingredients without accelerator, carbon black and aromatic oil. The effects of CBS/TMTD and TBBS/TMTD as accelerator systems were studied with different quantities and the best accelerator system was chosen. Then, the blends of N330 and N550 carbon blacks were added in different quantities and the properties of these samples were studied to determine the best carbon black blend. Finally, the effect of different quantities of aromatic oil was investigated and the optimized quantity of aromatic oil and the final properties of tire tread compound were defined. The mechanical and dynamical tests were carried out on appropriate samples to determine tensile strength, elongation-at-break, fatigue-to-failure, abrasion resistance, hardness, resilience, dynamical-mechanical properties and temperature rise due to the heat build-up. The results showed that the compound containing 0.8 phr CBS, 0.7 phr TMTD, 40 phr N330,20 phr N550 and 15 phr aromatic oils demonstrated the best properties.

  1. Mechanism of reactivation of the UV-inactivated cells of Escherichia coli by cell extracts of propionic acid bacteria

    International Nuclear Information System (INIS)

    Vorob'eva, L.I.; Khodzhaev, E.Y.; Ponomareva, G.M.

    1995-01-01

    Two mechanisms of reactivation of UV-inactivated Escherichia coli cells - photoreactivation (PhR) and reactivation by the dialyzate of cell extract of propionic acid bacteria - are shown to be different but not completely additive. PhR displays an insignificant negative effect on the reactivaton by active substances (peptides) of the dialyzate, whereas reactivation by dialyzate inhibits PhR. The maximal reactivation can be attained under complete PhR followed by the protective action of dialyzate. The dialyzate protects UV-irradiated E. coli cells with PolA, UvrA, and RecA mutations and Salmonella typhimurium TA 100 (UvrB) cells, and also exerts an antimutagenic effect on S. typhimurium TA 100. Protection by dialyzate is suggested to be due to restoration of the cell division mechanism damaged by UV irradiation. 14 refs., 3 figs., 5 tabs

  2. An information and communication technology-based centralized clinical trial to determine the efficacy and safety of insulin dose adjustment education based on a smartphone personal health record application: a randomized controlled trial.

    Science.gov (United States)

    Kim, Gyuri; Bae, Ji Cheol; Yi, Byoung Kee; Hur, Kyu Yeon; Chang, Dong Kyung; Lee, Moon-Kyu; Kim, Jae Hyeon; Jin, Sang-Man

    2017-07-18

    A Personal Health Record (PHR) is an online application that allows patients to access, manage, and share their health data. PHRs not only enhance shared decision making with healthcare providers, but also enable remote monitoring and at-home-collection of detailed data. The benefits of PHRs can be maximized in insulin dose adjustment for patients starting or intensifying insulin regimens, as frequent self-monitoring of glucose, self-adjustment of insulin dose, and precise at-home data collection during the visit-to-visit period are important for glycemic control. The aim of this study is to examine the efficacy and safety of insulin dose adjustment based on a smartphone PHR application in patients with diabetes mellitus (DM) and to confirm the validity and stability of an information and communication technology (ICT)-based centralized clinical trial monitoring system. This is a 24-week, open-label, randomized, multi-center trial. There are three follow-up measures: baseline, post-intervention at week 12, and at week 24. Subjects diagnosed with type 1 DM, type 2 DM, and/or post-transplant DM who initiate basal insulin or intensify their insulin regimen to a basal-bolus regimen are included. After education on insulin dose titration and prevention for hypoglycemia and a 1-week acclimation period, subjects are randomized in a 1:1 ratio to either an ICT-based intervention group or a conventional intervention group. Subjects in the conventional intervention group will save and send their health information to the server via a PHR application, whereas those in ICT-based intervention group will receive additional algorithm-based feedback messages. The health information includes level of blood glucose, insulin dose, details on hypoglycemia, food diary, and step count. The primary outcome will be the proportion of patients who reach an optimal insulin dose within 12 weeks of study enrollment, without severe hypoglycemia or unscheduled clinic visits. This clinical trial

  3. Dicty_cDB: VHK791 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ificant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL15...142916_1( BC142916 |pid:none) Danio rerio hypothetical LOC555303... 105 5e-21 BC171956_1( BC171956 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 103 1e-20 BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA ...(cDNA cl... 103 1e-20 (Q9JLC8) RecName: Full=Sacsin; 103 1e-20 AB006708_10( AB006

  4. Experiment list: SRX545935 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available rebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skil...ls. 22126564,95.3,7.1,23193 GSM1388150: H3K9/14ac WT P22 B2; Mus musculus; ChIP-Seq

  5. GenBank blastx search result: AK060220 [KOME

    Lifescience Database Archive (English)

    Full Text Available ative exons 18 and 18a, and alternative splice products, complete cds.|ROD ROD 2e-36 +3 ... ...AK060220 001-002-F01 AF071946.1 Mus musculus protein tyrosine kinase Tec (Tec) gene, exons 16 and 17, altern

  6. The personal health record paradox: health care professionals' perspectives and the information ecology of personal health record systems in organizational and clinical settings.

    Science.gov (United States)

    Nazi, Kim M

    2013-04-04

    Despite significant consumer interest and anticipated benefits, overall adoption of personal health records (PHRs) remains relatively low. Understanding the consumer perspective is necessary, but insufficient by itself. Consumer PHR use also has broad implications for health care professionals and organizational delivery systems; however, these have received less attention. An exclusive focus on the PHR as a tool for consumer empowerment does not adequately take into account the social and organizational context of health care delivery, and the reciprocal nature of patient engagement. The purpose of this study was to examine the experiences of physicians, nurses, and pharmacists at the Department of Veterans Affairs (VA) using an organizationally sponsored PHR to develop insights into the interaction of technology and processes of health care delivery. The conceptual framework for the study draws on an information ecology perspective, which recognizes that a vibrant dynamic exists among technologies, people, practices, and values, accounting for both the values and norms of the participants and the practices of the local setting. The study explores the experiences and perspectives of VA health care professionals related to patient use of the My HealtheVet PHR portal and secure messaging systems. In-depth interviews were conducted with 30 VA health care professionals engaged in providing direct patient care who self-reported that they had experiences with at least 1 of 4 PHR features. Interviews were transcribed, coded, and analyzed to identify inductive themes. Organizational documents and artifacts were reviewed and analyzed to trace the trajectory of secure messaging implementation as part of the VA Patient Aligned Care Team (PACT) model. Study findings revealed a variety of factors that have facilitated or inhibited PHR adoption, use, and endorsement of patient use by health care professionals. Health care professionals' accounts and analysis of organizational

  7. The dynamic relationship between emotional and physical states: an observational study of personal health records

    Directory of Open Access Journals (Sweden)

    Lee YS

    2017-02-01

    Full Text Available Ye-Seul Lee,1 Won-Mo Jung,1 Hyunchul Jang,2 Sanghyun Kim,2 Sun-Yong Chung,3 Younbyoung Chae1 1Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul, 2Mibyeong Research Center, Korean Institute of Oriental Medicine, Daejeon, 3Department of Neuropsychiatry, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea Objectives: Recently, there has been increasing interest in preventing and managing diseases both inside and outside medical institutions, and these concerns have supported the development of the individual Personal Health Record (PHR. Thus, the current study created a mobile platform called “Mind Mirror” to evaluate psychological and physical conditions and investigated whether PHRs would be a useful tool for assessment of the dynamic relationship between the emotional and physical conditions of an individual.Methods: Mind Mirror was used to collect 30 days of observational data about emotional valence and the physical states of pain and fatigue from 20 healthy participants, and these data were used to analyze the dynamic relationship between emotional and physical conditions. Additionally, based on the cross-correlations between these three parameters, a multilevel multivariate regression model (mixed linear model [MLM] was implemented.Results: The strongest cross-correlation between emotional and physical conditions was at lag 0, which implies that emotion and body condition changed concurrently. In the MLM, emotional valence was negatively associated with fatigue (β =-0.233, P<0.001, fatigue was positively associated with pain (β =0.250, P<0.001, and pain was positively associated with fatigue (β =0.398, P<0.001.Conclusion: Our study showed that emotional valence and one’s physical condition negatively influenced one another, while fatigue and pain positively affected each other. These findings suggest that the mind and body interact instantaneously, in

  8. 6,6'-(1E,1'E-((1R,2R-1,2-Diphenylethane-1,2-diylbis(azan-1-yl-1-ylidenebis(methan-1-yl-1-ylidenebis(2-tert-butyl-4-((trimethylsilylethynylphenol

    Directory of Open Access Journals (Sweden)

    David Díaz Díaz

    2013-03-01

    Full Text Available Functionalizable salen derivative, 6,6'-(1E,1'E-((1R,2R-1,2-diphenylethane-1,2-diylbis(azan-1-yl-1-ylidenebis(methan-1-yl-1-ylidenebis(2-tert-butyl-4-((trimethylsilyl ethyn-ylphenol (3, was synthesized by condensation between (1R,2R-1,2-diphenylethane-1,2-diamine (2 and 3-tert-butyl-2-hydroxy-5-((trimethylsilylethynyl benzaldehyde (1 under refluxing conditions. The title compound was characterized by 1H-NMR, 13C-NMR, FT-IR, high-resolution mass spectrometry, optical rotation and melting point determination.

  9. Sequence Classification: 889932 [

    Lifescience Database Archive (English)

    Full Text Available ctor, involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1; Gis1p || http://www.ncbi.nlm.nih.gov/protein/6320301 ...

  10. Dicty_cDB: VHD478 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 35 0.63 (Q9NZJ4) RecName: Full=Sacs...in; &AL157766_4( AL157766 |pid:none) 35 0.63 (Q9JLC8) RecName: Full=Sacsin; 35 0.63... BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 35 0.63 AL732351_6( AL732351 |pid:non

  11. Experiment list: SRX471836 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 0: ChIPseq GS WT Scml2; Mus musculus; ChIP-Seq source_name=Cultured germline stem cells || genotype/variatio...n=Wild-type || strain=CD1 x C57BL/6 || cell type=Cultured germline stem cells || chip antibody=Rabbit anti-S

  12. Experiment list: SRX977383 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NA 59099899,98.6,14.0,974 GSM1648634: input DNA day1; Mus musculus; ChIP-Seq source_name=reprogramming inter...mediate after 24hour of induction || cell type=reprogramming intermediate || genotype=Oct4-GFP/ Rosa26-M2rtT

  13. Preparation and properties of natural rubber reinforced with polydopamine-coating modified carbon nanotubes

    Directory of Open Access Journals (Sweden)

    Y-L. Lu

    2017-01-01

    Full Text Available Multi-walled carbon nanotubes (MWCNTs were functionalized by polydopamine (PDA-coating and mixed with natural rubber (NR via latex compounding. Compared with pristine MWCNTs, the surface of MWCNT-PDA was covered by an amorphous and nanometer-scale PDA layer which had a large amount of oxygenic and nitric functional groups. So the MWCNT-PDA showed a perfect dispersion in NR matrix. The tensile strength of NR/MWCNT-PDA (5 phr composites is 28.6 MPa, compared with the pure NR, which increased by 42%. For the electrical properties, when the content of MWCNTPDA or MWCNTs is 2 phr, the volume resistivity of NR/MWCNT-PDA composites falls to about 2.7·109 Ω·cm, compared with 3.3·1013 Ω·cm of NR/MWCNT composites. The thermal conductivity of NR composites increased only by 28.2% when 5 phr MWCNT-PDA was added. A model proposed by Nan was used to calculate the thermal conductivity of NR/MWCNT composites, and the calculated values were compared with the experimental values, the results showed that the interface thermal resistance is the main reason why MWCNTs could not significantly increase the thermal conductivity of natural rubber.

  14. Leisure time physical activity and health-related quality of life.

    Science.gov (United States)

    Vuillemin, Anne; Boini, Stéphanie; Bertrais, Sandrine; Tessier, Sabrina; Oppert, Jean-Michel; Hercberg, Serge; Guillemin, Francis; Briançon, Serge

    2005-08-01

    There are few data on the relationship between health-related quality of life (HRQoL) and leisure time physical activity (LTPA) in the general population. We investigated the relationships of meeting public health recommendations (PHR) for moderate and vigorous physical activity with HRQoL in French adult subjects. LTPA and HRQoL were assessed in 1998 in 2333 men and 3321 women from the SU.VI.MAX. cohort using the French versions of the Modifiable Activity Questionnaire (MAQ) and the SF-36 questionnaire, respectively. Relationship between LTPA and HRQoL was assessed using analysis of variance. Results from multivariate analysis showed that meeting physical activity recommended levels was associated with higher HRQoL scores (except in Bodily pain dimension for women): differences in mean HRQoL scores between subjects meeting or not PHR ranged from 2.4 (Mental health) to 4.5 (Vitality) and from 2.2 (Bodily pain) to 5.7 (Vitality) for women and men, respectively. Subjects meeting PHR for physical activity had better HRQoL than those who did not. Our data suggest that 30' of moderate LTPA per day on a regular basis may be beneficial on HRQoL. Higher intensity LTPA is associated with greater HRQoL. This emphasizes the importance to promote at least moderate physical activity.

  15. NCBI nr-aa BLAST: CBRC-CFAM-23-0010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-23-0010 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 0.0 91% ...

  16. NCBI nr-aa BLAST: CBRC-BTAU-01-3057 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-3057 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 0.0 90% ...

  17. Experiment list: SRX977396 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 99.3,8.4,86055 GSM1648647: H3K4me1 ChipSeq day11; Mus musculus; ChIP-Seq source_name=reprogramming intermedi...ate after 11days of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA

  18. Experiment list: SRX977397 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 98.9,8.9,45421 GSM1648648: H3K4me1 ChipSeq day15; Mus musculus; ChIP-Seq source_name=reprogramming intermedi...ate after 15days of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA

  19. AcEST: DK944957 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ant alignments: (bits) Value sp|Q82Y15|ORN_NITEU Oligoribonuclease OS=Nitrosomonas europaea G... 31 1.7 sp|Q9JLC8|SACS_MOUSE Sacsi...LVVTDAQLNTLAEAPVLVVHQPDDI 54 >sp|Q9JLC8|SACS_MOUSE Sacsin OS=Mus musculus GN=Sacs PE=1 SV=2 Length = 4582 Sc

  20. Arabidopsis CDS blastp result: AK065522 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK065522 J013026G17 At5g64830.1 programmed cell death 2 C-terminal domain-containin...g protein low similarity to SP|P46718 Programmed cell death protein 2 (Zinc finger protein Rp-8) {Mus muscul...us}; contains Pfam profile PF04194: Programmed cell death protein 2, C-terminal domain 3e-62 ...

  1. AcEST: DK951433 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6e-22 sp|Q9LM18|FB303_ARATH F-box protein At1g22220 OS=Arabidopsis tha... 100 1e-20 sp|O35902|DSG3_MOUSE Desmog...VGDCMYGEAVVALLKCKKNALEMNSF 314 >sp|O35902|DSG3_MOUSE Desmoglein-3 OS=Mus musculus GN=Dsg3 PE=2 SV=1 Length =

  2. Exploring Type-and-Identity-Based Proxy Re-Encryption Scheme to Securely Manage Personal Health Records

    NARCIS (Netherlands)

    Ibraimi, L.; Gangopadhyay, Aryya; Tang, Qiang; Hartel, Pieter H.; Jonker, Willem

    2010-01-01

    Commercial Web-based Personal-Health Record (PHR) systems can help patients to share their personal health records (PHRs) anytime from anywhere. PHRs are very sensitive data and an inappropriate disclosure may cause serious problems to an individual. Therefore commercial Web-based PHR systems have

  3. Sabiduría práctica: en la intención de una vida buena con y para los otros

    Directory of Open Access Journals (Sweden)

    Fredy Hernán Prieto Galindo

    2012-11-01

    Full Text Available This paper pretends to examine the Ricoeur’s notion of Phrónesis, from the point of view of an individual ethics, aiming to show that it is much more than just a kind of mediation for moral conflicts. The place and role of this phrónesis in at every level (teleological, deontological and prudencial of Ricoeur´s «small ethics» will be examined. In doing so, a dialectic between the desire and the rule will be exposed, as well as the relation between ipseity and alterity, both according to the concept of phrónesis.

  4. Characterization Study of EPDM Rubber Vulcanized by Gamma Radiation in The Presence of Epoxidized Soybean Oil

    International Nuclear Information System (INIS)

    Radi, H.; Mousaa, I.M.

    2015-01-01

    Composites based on ethylene-propylene-diene rubber (EPDM) were prepared. EPDM loaded with 40 phr fumed silica in the presence of different concentrations of epoxidized soybean oil ranging between 4 and 8 phr. The composites were subjected to various gamma irradiation doses up to 200 kGy. The physical, mechanical and thermal properties of rubber composition as a function of irradiation dose were investigated. Gamma irradiation led to a significant improvement in the properties of the all compositions. Besides, an improvement in the mechanical properties was attained with the addition of 4 phr of epoxidized soybean oil.

  5. Multilayer Graphene/Carbon Black/Chlorine Isobutyl Isoprene Rubber Nanocomposites

    Directory of Open Access Journals (Sweden)

    Daniele Frasca

    2016-03-01

    Full Text Available High loadings of carbon black (CB are usually used to achieve the properties demanded of rubber compounds. In recent years, distinct nanoparticles have been investigated to replace CB in whole or in part, in order to reduce the necessary filler content or to improve performance. Multilayer graphene (MLG is a nanoparticle made of just 10 graphene sheets and has recently become commercially available for mass-product nanocomposites. Three phr (part for hundred rubbers of MLG are added to chlorine isobutyl isoprene rubber (CIIR/CB composites in order to replace part of the CB. The incorporation of just 3 phr MLG triples the Young’s modulus of CIIR; the same effect is obtained with 20 phr CB. The simultaneous presence of three MLG and CB also delivers remarkable properties, e.g. adding three MLG and 20 phr CB increased the hardness as much as adding 40 phr CB. A comprehensive study is presented, showing the influence on a variety of mechanical properties. The potential of the MLG/CB combination is illustrated to reduce the filler content or to boost performance, respectively. Apart from the remarkable mechanical properties, the CIIR/CB/MLG nanocomposites showed an increase in weathering resistance.

  6. Asylum grant rates following medical evaluations of maltreatment among political asylum applicants in the United States.

    Science.gov (United States)

    Lustig, Stuart L; Kureshi, Sarah; Delucchi, Kevin L; Iacopino, Vincent; Morse, Samantha C

    2008-02-01

    Although many individuals applying for political asylum allege maltreatment and sometimes torture in their countries of origin, the utility of medical evaluations in asylum adjudication has not been documented. This study compares the asylum grant rate among US asylum seekers who received medical evaluations from Physicians for Human Rights (PHR), with rates among asylum seekers who did not receive PHR evaluations. Retrospective analysis was carried out on all asylum cases referred to PHR between 2000 and 2004 for medical evaluations for which adjudication outcome was available. Basic demographic information was obtained: age, sex, country of origin, English language ability, US region where adjudication occurred, whether legal representation was pro bono, type of evaluation, provision of oral court testimony, and whether asylum seekers were in detention. Cases were analyzed descriptively and with chi square tests. Between 2000 and 2004, 1663 asylum seekers received medical evaluations from PHR; the adjudication status (either granted or denied) was determined in 746 cases at the time of the study. Of these cases, 89% were granted asylum, compared to the national average of 37.5% among US asylum seekers who did not receive PHR evaluations. Medical evaluations may be critical in the adjudications of asylum cases when maltreatment is alleged.

  7. Importance-Performance Analysis of Personal Health Records in Taiwan: A Web-Based Survey.

    Science.gov (United States)

    Rau, Hsiao-Hsien; Wu, Yi-Syuan; Chu, Chi-Ming; Wang, Fu-Chung; Hsu, Min-Huei; Chang, Chi-Wen; Chen, Kang-Hua; Lee, Yen-Liang; Kao, Senyeong; Chiu, Yu-Lung; Wen, Hsyien-Chia; Fuad, Anis; Hsu, Chien-Yeh; Chiu, Hung-Wen

    2017-04-27

    Empowering personal health records (PHRs) provides basic human right, awareness, and intention for health promotion. As health care delivery changes toward patient-centered services, PHRs become an indispensable platform for consumers and providers. Recently, the government introduced "My health bank," a Web-based electronic medical records (EMRs) repository for consumers. However, it is not yet a PHR. To date, we do not have a platform that can let patients manage their own PHR. This study creates a vision of a value-added platform for personal health data analysis and manages their health record based on the contents of the "My health bank." This study aimed to examine consumer expectation regarding PHR, using the importance-performance analysis. The purpose of this study was to explore consumer perception regarding this type of a platform: it would try to identify the key success factors and important aspects by using the importance-performance analysis, and give some suggestions for future development based on it. This is a cross-sectional study conducted in Taiwan. Web-based invitation to participate in this study was distributed through Facebook. Respondents were asked to watch an introductory movie regarding PHR before filling in the questionnaire. The questionnaire was focused on 2 aspects, including (1) system functions, and (2) system design and security and privacy. The questionnaire would employ 12 and 7 questions respectively. The questionnaire was designed following 5-points Likert scale ranging from 1 ("disagree strongly") to 5 ("Agree strongly"). Afterwards, the questionnaire data was sorted using IBM SPSS Statistics 21 for descriptive statistics and the importance-performance analysis. This research received 350 valid questionnaires. Most respondents were female (219 of 350 participants, 62.6%), 21-30 years old (238 of 350 participants, 68.0%), with a university degree (228 of 350 participants, 65.1%). They were still students (195 out of 350

  8. Dicty_cDB: VHE123 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nificant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &AL157766_4( AL157766 |pid:none) 105 4e-21 ...none) Micromonas sp. RCC299 chromosome... 103 1e-20 BC138482_1( BC138482 |pid:none) Mus musculus sacsi...n, mRNA (cDNA cl... 103 1e-20 (Q9JLC8) RecName: Full=Sacsin; 103 1e-20 BC171956_1( BC17...1956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl... 103 1e-20 AB006708_10( AB006708 |pid:none) Arabidopsis

  9. Experiment list: SRX897943 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 153,97.9,13.9,15440 GSM1624628: ChIP seq Renilla Sox2 IP day3; Mus musculus; ChIP-Seq source_name=OKSM reprogramming... intermediates from Mouse Embryonic Fibroblasts || strain=Black6-129X1/SvJ || cell type=OKSM reprogramming

  10. Experiment list: SRX192262 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available produced at one birth by a viviparous animal. 13178340,75.6,7.8,715 GSM1016431: B H3K4me2 vehicle donor1; Mu...s musculus; ChIP-Seq source_name=whole liver extract || age=29-32 days || chip antibody=H3K4me2 || treament=vehicle

  11. NCBI nr-aa BLAST: CBRC-GACU-23-0054 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-23-0054 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 4e-55 36% ...

  12. NCBI nr-aa BLAST: CBRC-PMAR-01-0530 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0530 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 6e-69 42% ...

  13. NCBI nr-aa BLAST: CBRC-DRER-07-0075 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-07-0075 sp|Q6TCG2|PAQR9_MOUSE Progestin and adipoQ receptor family member 9 (Progesti...n and adipoQ receptor family member IX) gb|AAR08386.1| progestin and adipoQ receptor family member IX [Mus musculus] Q6TCG2 9e-57 36% ...

  14. Experiment list: SRX977393 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 98.8,8.9,31577 GSM1648644: H3K4me1 ChipSeq day3; Mus musculus; ChIP-Seq source_name=reprogramming intermedia...te after 3days of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA t

  15. Experiment list: SRX977395 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 98.7,7.3,62664 GSM1648646: H3K4me1 ChipSeq day7; Mus musculus; ChIP-Seq source_name=reprogramming intermedia...te after 7days of induction || cell type=reprogramming intermediate || genotype=H3K4me1-GFP/ Rosa26-M2rtTA t

  16. Dicty_cDB: VHB767 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available vs Protein Score E Sequences producing significant alignments: (bits) Value (Q9NZJ4) RecName: Full=Sacsin; &...AL157766_4( AL157766 |pid:none) 111 7e-23 BC171956_1( BC171956 |pid:none) Mus musculus sacsin, mRNA (cDNA cl...... 109 3e-22 BC138482_1( BC138482 |pid:none) Mus musculus sacsin, mRNA (cDNA cl...... 108 3e-22 (Q9JLC8) RecName: Full=Sacsin; 108 3e-22 BC142916_1( BC142916 |pid:none) Danio rerio hypothetic

  17. Studies on gamma irradiated rubber materials

    Science.gov (United States)

    Lungu, I. B.; Stelescu, M. D.; Cutrubinis, M.

    2018-01-01

    Due to the increase in use and production of polymer materials, there is a constant pressure of finding a solution to more environmental friendly composites. Beside the constant effort of recycling used materials, it seems more appropriate to manufacture and use biodegradable and renewable row materials. Natural polymers like starch, cellulose, lignin etc are ideal for preparing biodegradable composites. Some of the dynamic markets that use polymer materials are the food and pharmaceutical industries. Because of their desinfastation and sometimes sterility requirements, different treatment processes are applied, one of it being radiation treatment. The scope of this paper is to analyze the mechanical behaviour of rubber based materials irradiated with gamma rays at four medium doses, 30.1 kGy, 60.6 kGy, 91 kGy and 121.8 kGy. The objectives are the following: to identify the optimum radiation dose in order to obtain a good mechanical behaviour and to identify the mechanical behaviour of the material when adding different quantities of natural filler (20 phr, 60 phr and 100 phr).

  18. Polyvinyl Chloride / Attapulgite / Micro-crystalline Cellulose (MCC Composites Preparation and Analysis of the Role of MCC as a Compatibilizer

    Directory of Open Access Journals (Sweden)

    Bo Wang

    2015-09-01

    Full Text Available To improve the performance of polyvinyl chloride (PVC, composites incorporating polyvinyl chloride (PVC, attapulgite nanoparticles (ANPs, and microcrystalline cellulose (MCC were successfully prepared. The composites had higher vicat softening temperatures (VSTs and the MCC had a great influence on mechanical properties of the composites. When MCC was added from 0 to 5 per hundred parts of PVC (phr, the mechanical properties of the composites increased, but the mechanical properties of the composites decreased when the MCC was more than 5 phr. The tensile breaking stress, tensile strength, and impact strength were maximized with increases of 19.76 N (4.1%, 29.66 MPa (15.5%, and 13.8 MPa (7% when 5 phr MCC was added. Infrared spectral analysis indicated that MCC and ANPs were present in the composites. Scanning electron microscopy showed that the composites system was distributed into two phases, which indicated that MCC in composites was dissolved in the PVC matrix, and some of MCC coated the surface of ANPs as a compatibilizer. Overall, this study provided a promising method for PVC modification to improve its performance.

  19. PLA and two components silicon rubber blends aiming for frozen foods packaging applications

    Science.gov (United States)

    Meekum, Utai; Khiansanoi, Apichart

    2018-03-01

    Designing of PLA and two components silicone rubber blends was studies. Frozen food packaging application is the main ultimate aim. The statistical method using 23 DOE was conducted. The standard testing methods, in particular impact testing at sub-zero temperature, were performed. The preliminary blend formula comprised 1.0 phr of silane and polyester polyols, respectively, was initially resolved. Then, the optimize the silicone portion in the blends was determined. Blending formula using 8.0 phr of silicone with respect to PLA matrix gave rise to the overall satisfactory properties. 3. TETA was used as the silicone curing agent and reactively blended onto the ingredients. TETA at 0.4 phr, with respect to the silicone, enhanced the mechanical properties, especially flexibility and toughness, of the PLA/silicone blend. Exceeding the optimal TETA loading would cause the chain scission and also the dilution effects. Hence, marginal inferior properties of the blends were be experienced. The preliminary biodegradability investigation found that the PLA/silicone blend initially triggered at the second week. Its degradation rate was likely to be faster than neat PLA.

  20. Crystal structures of 2,2′-bipyridin-1-ium 1,1,3,3-tetracyano-2-ethoxyprop-2-en-1-ide and bis(2,2′-bipyridin-1-ium 1,1,3,3-tetracyano-2-(dicyanomethylenepropane-1,3-diide

    Directory of Open Access Journals (Sweden)

    Zouaoui Setifi

    2015-05-01

    Full Text Available In 2,2′-bipyridin-1-ium 1,1,3,3-tetracyano-2-ethoxyprop-2-en-1-ide, C10H9N2+·C9H5N4O−, (I, the ethyl group in the anion is disordered over two sets of atomic sites with occupancies 0.634 (9 and 0.366 (9, and the dihedral angle between the ring planes in the cation is 2.11 (7°. The two independent C(CN2 groups in the anion make dihedral angles of 10.60 (6 and 12.44 (4° with the central propenide unit, and the bond distances in the anion provide evidence for extensive electronic delocalization. In bis(2,2′-bipyridin-1-ium 1,1,3,3-tetracyano-2-(dicyanomethylenepropane-1,3-diide [alternative name bis(2,2′-bipyridin-1-ium tris(dicyanomethylenemethanediide], 2C10H9N2+·C10N62− (II, the dihedral angles between the ring planes in the two independent cations are 7.7 (2 and 10.92 (17°. The anion exhibits approximate C3 symmetry, consistent with extensive electronic delocalization, and the three independent C(CN2 groups make dihedral angles of 23.8 (2, 27.0 (3 and 27.4 (2° with the central plane. The ions in (I are linked by an N—H...N hydrogen bond and the resulting ion pairs are linked by two independent C—H...N hydrogen bonds, forming a ribbon containing alternating R44(18 and R44(26 rings, where both ring types are centrosymmetric. The ions in (II are linked by two independent N—H...N hydrogen bonds and the resulting ion triplets are linked by a C—H...N hydrogen bond, forming a C21(7 chain containing anions and only one type of cation, with the other cation linked to the chain by a further C—H...N hydrogen bond.