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Sample records for muria secara apn

  1. SPIRITUALITAS MURIA: AKOMODASI TRADISI DAN WISATA

    OpenAIRE

    Ahmad Falah

    2012-01-01

    Tradition of “nyekar” which is very familiar among Javanese society, especially in the grave yard Muria Kudus Central Java. This research revealed that most of the visitor of the grave are for getting tabarrukan , some kind safety and ease in any effort in their life, mastering any knowledge, and linuwih, getting for healing from any desease, and asking for blessing for their descendants. The visit to the grave impacted much to the society living, both economically and socially. In the perspe...

  2. Gunung api maar di Semenanjung Muria

    Directory of Open Access Journals (Sweden)

    Sutikno Bronto

    2014-06-01

    Full Text Available http://dx.doi.org/10.17014/ijog.vol2no1.20074Three maars are well identifi ed in the Muria Peninsula, i.e. Bambang Maar, Gunungrowo Maar, and Gembong Maar. The maars were formed by monogenetic volcanic eruptions due to the interaction between heat source (magma, groundwater and calcareous basement rocks. This interaction is able to produce very high pressure of gas and steam causing phreatic explosions, followed by phreatomagmatic- or even magmatic explosions and ended by a lava extrusion. Satellite image analyses have recognized twelve circular features, comprising Bambang Maar, Gunungrowo Maar, and Gembaong Maar. Phisiographically, these maars are characterized by circular depressions which are surrounded by hills that are gently sloping down away from the crater or having a radier pattern morphology. Outcrops and drilling core in the circular areas that are considered as volcanic maars are lava fl ows, pyroclastic breccias, lapillistones, and tuffs, located far away from the eruption centres of Muria and Genuk Volcanoes. One of the circular features, i.e. Jepara Circular Feature, is also supported by negative anomaly (<30 mgal showing a circular pattern. In the future, a maar volcano could possibly erupt depending on the tectonic reactivity in the region.  

  3. INTERPRETASI STRUKTUR BAWAH PERMUKAAN GUNUNG MURIA MENGGUNAKAN ANALISA DATA GRAVITASI

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    Nasrun Balulu

    2012-03-01

    Full Text Available A geophysical survey using gravity method was carried out in Muria  volcano. This research aimed to interpret the subsurface geology condition of the research area based on gravity data analysis. Research area is (47,6 x 45 km2    with 222 observation  points. Gravitymeter  LaCoste & Romberg type G-1118 MVR was used to    measure the  gravity field, and differential method of Global Positioning System (GPS was used to measure the position and elevation. Data processing was performed to gain complete Bouguer  anomaly of both residual and  regional. Bouguer density using graphical method resulted to the value of 2.67 gr/cm3.  Transformation to a horizontal plane was performed obtain by using equivalent mass method with horizontal level’s height of 1602 meters beyond the spheroid reference and equivalent The separation of regional anomaly and residual  anomaly  was  generated  through  upward  continuation  method  and  resulted  to  regional anomaly in the height of  15000 meters up the spheroid reference. The residual anomaly acquired by subtract the regional anomaly toward the complete Bouguer anomaly in a horizontal plane. The subsurface modelling of Muria  volcano and its surrounding was acquired from  Grav2DC for Windows. The result shows that Muria volcano and its surrounding was regionally controlled by rock resulted from volcanic processes as lava (density 2,91 gr/cm3. This volcanic rock due to the gravity collapse performed a caldera filled by andesit (density 2,58 gr/cm3. Another rock which is deposited in  Muria  volcano  are  tuff  from  Muria  Tuff  (density  2,4  gr/cm3,  tuff  sandstone  from  Patiayam formation (density 2,5 gr/cm3,  limestone from Bulu formation (density 2,7 gr/cm3, and  limestone from Ngrayong formation (density 2,8 gr/cm3. Keywords:Bouguer anomaly, Regional anomaly, Residual anomaly,  Modeling, Subsurface stracture.

  4. Constructing new APN functions from known ones

    DEFF Research Database (Denmark)

    Budaghyan, L.; Carlet, C.; Leander, Gregor

    2009-01-01

    We present a method for constructing new quadratic APN functions from known ones. Applying this method to the Gold power functions we construct an APN function x(3) + tr(x(9)) over F2(n). It is proven that for n >= 7 this function is CCZ-inequivalent to the Gold functions, and in the case n = 7 i...

  5. Radiological dose in Muria peninsula from SB-LOCA event

    Science.gov (United States)

    Sunarko; Suud, Zaki

    2017-01-01

    Dose assessment for accident condition is performed for Muria Peninsula region using source-term from Three-Mile Island unit 2 SB-LOCA accident. Xe-133, Kr-88, 1-131 and Cs-137 isotopes are considered in the calculation. The effluent is assumed to be released from a 50 m stack. Lagrangian particle dispersion method (LPDM) employing non-Gaussian dispersion coefficient in 3-dimensional mass-consistent wind-field is employed to obtain periodic surface-level concentration which is then time-integrated to obtain spatial distribution of ground-level dose. In 1-hour simulation, segmented plumes with 60 seconds duration with a total of 18.000 particles involved. Simulations using 6-hour worst-case meteorological data from Muria peninsula results in a peak external dose of around 1.668 mSv for low scenario and 6.892 mSv for high scenario in dry condition. In wet condition with 5 mm/hour and 10 mm/hour rain for the whole duration of the simulation provides only minor effect to dose. The peak external dose is below the regulatory limit of 50 mSv for effective skin dose from external gamma exposure.

  6. MENGINTERPRETASI SECARA PRODUKTIF

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    M. Dwi Marianto

    2002-01-01

    Full Text Available This article composes of adaptations of%2C and quotations of%2C several writings on meaning and text of Ferdinand de Saussure%2C Stuart Hall%2C Paul Ricoeur%2C and a number of practical ways of interpreting as written by Terry Barrett%2C as well as on physical characteristics of creatures for survival by Peter Forbes. Nevertheless%2C the core subject of this article is about a text which is autonomous. A text can be a work of art / design / craft and any work of art. The meaning of a text never be static%2C fixed and frozen%2C it always changes%2C depends on the way it is seen. Therefore%2C it can be said that interpreting is not merely reproductive%2C more than it is productive. The purpose of this article is to emphasize the significant points in interpreting and giving meaning onto a work of art/ design / craft as suggested by the aforementioned scholars. The practical intention is to enlighten and empower readers%2C especially those who want to interpret work of art / design / craft productively%2C creatively and imaginatively. Abstract in Bahasa Indonesia : Artikel ini disusun dari beberapa tulisan saduran atas%2C dan petikan dari%2C beberapa tulisan tentang makna dan teks dari Ferdinand de Saussure%2C Stuart Hall%2C Paul Ricoeur%2C beberapa cara praktis menginterpretasi yang ditulis oleh Terry Barrett%2C serta tentang karakterristik fisikal dari makhluk hidup yang berfungsi untuk survival dari Peter Forbes. Namun pokok pembicaraan utama adalah mengenai teks itu sifatnya otonom. Teks itu bisa karya seni/desain/kriya dan lain-lain. Makna sebuah teks tidak pernah baku dan beku%2C selalu bergerak%2C dan sangat bergantung pada bagaimana ia dipandang. Makanya%2C dapat dikatakan%2C bahwa dalam menginterpretasi si pembaca/pemirsa tidak cuma mereproduksi%2C melainkan juga dapat memproduksi makna. Menginterpretasi oleh karenanya sama dengan merepresentasi%2C bisa dilakukan secara kreatif dan produktif. Maksud tulisan ini adalah menggarisbawahi butir

  7. PEMISAHAN Zr – Hf SECARA SINAMBUNG MENGGUNAKAN MIXER SETTLER

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    Dwi Biyantoro

    2017-01-01

    Full Text Available ABSTRAK PEMISAHAN Zr – Hf SECARA SINAMBUNG MENGGUNAKANMIXER SETTLER. Telah dilakukan pemisahanZr – Hf secara sinambung menggunakan pengaduk pengenap (mixer settler 16 stage. Larutan umpan adalah zirkon nitrat dengan kadar Zr = 30786 ppm dan Hf = 499 ppm. Ekstraktan dipakai adalah solven 60 % TBP dalam kerosen dan larutan scrubbingyang dipakai adalah asam nitrat 1 M. Umpan masuk pada stageke 5 dikontakkan secara berlawanan arah dengan solven masuk pada stage ke 16 dan larutan scrubbing masuk pada stage ke 1. Tujuan penelitian ini adalah memisahkan unsur Zr dan Hf dari hasil olah pasir zirkon menggunakan solven TBP dengan alat mixer settler16 stage. Analisis umpan dan hasil proses pemisahan untuk zirkonium (Zr dilakukan dengan menggunakan alat pendar sinar-X, sedangkananalisis unsur hafnium (Hf menggunakan Analisis Pengaktifan Neutron (APN. Parameter penelitian dilakukan dengan variasi keasaman asam nitrat dalam umpan dan variasi waktu pada berbagai laju pengadukan. Hasil penelitian pemisahan unsur Zr dengan Hf diperolehkondisi optimum pada keasaman umpan 4 N HNO3, keseimbangan dicapai setelah 3jam dan laju pengadukan 3300 rpm. Hasil ekstrak  unsur zirkon (Zr diperoleh kadar sebesar 28577 ppm dengan efisiensi 92,76 % serta kadar pengotor hafnium (Hf sebesar 95 ppm. Kata Kunci: pemisahan Zr, Hf, ekstraksi, mixer settler, alat pendar sinar-X, APN. ABSTRACT SEPARATION of Zr - Hf CONTINUOUSLY USE THE MIXER SETTLER. Separation of Zr - Hf continuously using mixer settler 16 stage has been done. The feed solution is zircon nitrate concentration of Zr = 30786 ppm  and Hf = 499 ppm. As the solvent used extractant 60 % TBP in 40 % kerosene. Nitric acid solution used srubbing 1 M. The feed entered into stage to 5 is contacted with solvents direction on the stage to 16 and the scrubbing solution enter the stage to 1. The purpose of this study is to separate Zr and Hf of the results from the process of zircon sand using solvent TBP using 16 stage

  8. Geology and Origin of Europa's Mitten Feature (Murias Chaos)

    Science.gov (United States)

    Figueredo, P. H.; Chuang, F. C.; Rathbun, J.; Kirk, R. L.; Greeley, R.

    2002-01-01

    The "Mitten" (provisionally named Murias Chaos by the International Astronomical Union) is a region of elevated chaos-like terrain in the leading hemisphere of Europa. Its origin had been explained under the currently debated theories of melting through a thin lithosphere or convection within a thick one. Galileo observations reveal several characteristics that suggest that the Mitten is distinct from typical chaos terrain and point to a different formational process. Photoclinometric elevation estimates suggest that the Mitten is slightly elevated with respect to the surrounding terrain; geologic relations indicate that it must have raised significantly from the plains in its past, resembling disrupted domes on Europa's trailing hemisphere. Moreover, the Mitten material appears to have extruded onto the plains and flowed for tens of kilometers. The area subsequently subsided as a result of isostatic adjustment, viscous relaxation, and/or plains loading. Using plate flexure models, we estimated the elastic lithosphere in the area to be several kilometers thick. We propose that the Mitten originated by the ascent and extrusion of a large thermal diapir. Thermal-mechanical modeling shows that a Mitten-sized plume would remain sufficiently warm and buoyant to pierce through the crust and flow unconfined on the surface. Such a diapir probably had an initial radius between 5 and 8 km and an initial depth of 20-40 km, consistent with a thick-lithosphere model. In this scenario the Mitten appears to represent the surface expression of the rare ascent of a large diapir, in contrast to lenticulae and chaos terrain, which may form by isolated and clustered small diapirs, respectively.

  9. Geology and origin of Europa's "Mitten" feature (Murias Chaos)

    Science.gov (United States)

    Figueredo, P.H.; Chuang, F.C.; Rathbun, J.; Kirk, R.L.; Greeley, R.

    2002-01-01

    The "Mitten" (provisionally named Murias Chaos by the International Astronomical Union) is a region of elevated chaos-like terrain in the leading hemisphere of Europa. Its origin had been explained under the currently debated theories of melting through a thin lithosphere or convection within a thick one. Galileo observations reveal several characteristics that suggest that the Mitten is distinct from typical chaos terrain and point to a different formational process. Photoclinometric elevation estimates suggest that the Mitten is slightly elevated with respect to the surrounding terrain; geologic relations indicate that it must have raised significantly from the plains in its past, resembling disrupted domes on Europa's trailing hemisphere. Moreover, the Mitten material appears to have extruded onto the plains and flowed for tens of kilometers. The area subsequently subsided as a result of isostatic adjustment, viscous relaxation, and/or plains loading. Using plate flexure models, we estimated the elastic lithosphere in the area to be several kilometers thick. We propose that the Mitten originated by the ascent and extrusion of a large thermal diapir. Thermal-mechanical modeling shows that a Mitten-sized plume would remain sufficiently warm and buoyant to pierce through the crust and flow unconfined on the surface. Such a diapir probably had an initial radius between 5 and 8 km and an initial depth of 20-40 km, consistent with a thick-lithosphere model. In this scenario the Mitten appears to represent the surface expression of the rare ascent of a large diapir, in contrast to lenticulae and chaos terrain, which may form by isolated and clustered small diapirs, respectively.

  10. Entrepreneurship: Assessing the Readiness of the New Jersey APN Workforce.

    Science.gov (United States)

    Cadmus, Edna; Johansen, Mary L; Zimmer, Phyllis Arn; Knowlton, David L

    There is an unprecedented opportunity to move advanced practice nurses (APNs) into primary care settings at a steady rate over the next 5 to 8 years. In addition, the opportunity for nurse-owned or nurse-led practices has never been greater. However, many APNs currently work in a structured environment where the employer focuses on the business aspects of the practice and the APN focuses primarily on clinical care. Often APNs are unaware of the entrepreneurial contribution they make to the practice. A Needs Assessment Survey was developed to better understand business and practice management knowledge and skills of APNs in New Jersey. The survey included 14 categories for competency development. Twelve of the 14 categories showed that APNs were at a novice or an advanced beginner level. APNs need to demonstrate their value and take a lead to help solve primary care access issues. This can only be accomplished if APNs are willing to seize the opportunity and overcome barriers and knowledge gaps through both formal and informal education to step out of their traditional positions into more independent roles.

  11. Communication contention in APN list scheduling algorithm

    Institute of Scientific and Technical Information of China (English)

    TANG XiaoYong; LI KenLi; PADUA Divid

    2009-01-01

    Task scheduling is an essential aspect of parallel process system. This NP-hard problem assumes fully connected homogeneous processors and ignores contention on the communication links. However, as arbitrary processor network (APN), communication contention has a strong influence on the execution time of a parallel application. This paper investigates the Incorporation of contention awareness into task scheduling. The innovation is the idea of dynamically scheduling edges to links, for which we use the earliest finish communication time search algorithm based on shortest-path search method. The other novel idea proposed in this paper is scheduling priority based on recursive rank computation on heterogeneous arbitrary processor network. In the end, to reduce time complexity of algorithm, a parallel algorithm is proposed and speedup O(PPE) is achieved. The comparison study, based on both randomly generated graphs and the graphs of some real applications, shows that our scheduling algorithm significantly surpasses classic and static communication contention awareness algorithm, especially for high data transmission rate parallel application.

  12. Evaluation of trace elements distribution in water, sediment, soil and cassava plant in Muria peninsula environment by NAA method

    Energy Technology Data Exchange (ETDEWEB)

    Muryono, H.; Sumining; Agus Taftazani; Kris Tri Basuki; Sukarman, A. [Yogyakarta Nuclear Research Center, Yogyakarta (Indonesia)

    1999-10-01

    The evaluation of trace elements distribution in water, sediment, soil and cassava plant in Muria peninsula by NAA method were done. The nuclear power plant (NPP) and the coal power plant (CPP) will be built in Muria peninsula, so, the Muria peninsula is an important site for samples collection and monitoring of environment. River-water, sediment, dryland-soil and cassava plant were choosen as specimens samples from Muria peninsula environment. The analysis result of trace elements were used as a contributed data for environment monitoring before and after NPP was built. The trace elements in specimens of river-water, sediment, dryland-soil and cassava plant samples were analyzed by INAA method. It was found that the trace elements distribution were not evenly distributed. Percentage of trace elements distribution in river-water, sediment, dryland-soil and cassava leaves were 0.00026-0.037% in water samples, 0.49-62.7% in sediment samples, 36.29-99.35% in soil samples and 0.21-99.35% in cassava leaves. (author)

  13. Ethnobotany of utilization, role, and philosopical meaning of parijoto (Medinilla, spp) on Mount Muria in Kudus Regency, Central Java

    Science.gov (United States)

    Hanum, Alima Saida; Prihastanti, Erma; Jumari

    2017-08-01

    One of Indonesian plant species which has not been fully explored in terms of pharmacology is parijoto plant (Medinilla,spp). Medinilla,spp can be found on Mount Muria in Kudus regency, Central Java. This plant can be used as a traditional medicine which is derived from plants as a manifestation of active community participation in solving the healthy problems. Medinilla,spp is closely associated with the ethnic-majority area of mount Muria. Its utilization is still limited as the fruit to be consumed by pregnant women. It is necessary to do in-depth research as part of an effort in the utilization and conservation for the future. The purposes of this study were to determine the utilization, role, and philosophical meaning of Medinilla,spp. This research took place in three villages, they were Japan, Colo, and Pandak. Questionnaire and semi-structured interviews with community and key informants were used in this research. It was found out that Medinilla, spp majority were utilized as the fruit to be consumed by pregnant women and also used as medicine for diarrhea, mouth sores, anti inflammatory, anti-cancer, and anti-bacterial. The preparations process of traditional medical plants were by boiling (70%), way of brewing (15%), and consuming it freshly (15%). The composition of the ingredients were 90% used single material in the form of Medinilla,spp and 10% used addition ingredients such as ginger and turmeric. The philosophical meaning of Medinilla,spp for the people in Mount Muria region was that Medinilla, spp was not merely the fruit to be consumed by pregnant women, but Medinilla, spp was also considered as the fruit to heal various diseases. It referred to a belief that after salah prayer before hand by Sunan Muria, all diseases can be cured.

  14. Knowledge gaps regarding APN roles: what hospital decision-makers tell us.

    Science.gov (United States)

    Carter, Nancy; Dobbins, Maureen; Ireland, Sandra; Hoxby, Heather; Peachey, Gladys; DiCenso, Alba

    2013-12-01

    The implementation of advanced practice nursing (APN) roles can yield improvements in patient and health system outcomes, and supportive leadership is integral in facilitating the implementation of such roles. The purpose of this study was to explore the awareness and understanding of APN roles among hospital decision-makers, and to learn about the information they require and the ways in which they prefer to receive that information. Fifteen administrators and leaders from two multi-site acute care organizations were interviewed. Their practical knowledge of APN roles was based on experience developing the roles or working with APNs in hospital programs. The most common sources of APN information were internal contacts (i.e., APNs) and documents from nursing organizations. Participants reported difficulty distinguishing between the roles of nurse practitioners (NPs) and clinical nurse specialists (CNSs), and identified knowledge regarding CNS roles as their greatest need. They required specific information regarding the "value-added" benefits offered by an APN role. Strategies to address the knowledge gaps of healthcare leaders are urgently needed in order to support the implementation of new APN roles and to sustain existing ones.

  15. The analgesic activity of Bestatin as a potent APN inhibitor

    Directory of Open Access Journals (Sweden)

    Mei-Rong Jia

    2010-06-01

    Full Text Available Bestatin, a small molecular weight dipeptide, is a potent inhibitor of various aminopeptidases as well as LTA4 hydrolase. Various physiological functions of Bestatin have been identified, viz.: (1 an immunomodifier for enhancing the proliferation of normal human bone marrow granulocyte–macrophage progenitor cells to form CFU-GM colonies; Bestatin exerts a direct stimulating effect on lymphocytes via its fixation on the cell surface and an indirect effect on monocytes via aminopeptidase B inhibition of tuftsin catabolism; (2 an immunorestorator and curative or preventive agent for spontaneous tumor; Bestatin alone or its combination with chemicals can prolongate the disease-free interval and survival period in adult acute or chronic leukemia, therefore, it was primarily marketed in 1987 in Japan as an anticancer drug and servers as the only marketed inhibitor of Aminopeptidase N (APN/CD13 to cure leukemia to date; (3 a pan-hematopoietic stimulator and restorator; Bestatin promotes granulocytopoiesis and thrombocytopoiesis in vitro and restores them in myelo-hypoplastic men; (4 an inhibitor of several natural opioid peptides. Based on the knowledge that APN can cleave several bioactive neuropeptides such as Met-enkaphalins, Leu-enkaphalins, β-Endorphin, and so on, the antiaminopeptidase action of Bestatin also allows it to protect endopeptides against their catabolism, exhibiting analgesic activity. Although many scientific studies and great accomplishments have been achieved in this field, a large amount of problems are unsolved. This article reviews the promising results obtained for future development of the analgesic activity of Bestatin that can be of vital interest in a number of severe and chronic pain syndromes.

  16. From expert to novice: The unnerving transition from experienced RN to neophyte APN.

    Science.gov (United States)

    Forbes, Vashti Jude; Jessup, Anna Loomis

    2004-06-01

    Following analysis of a critical incident, we discuss role identity stressors during knowledge acquisition. Specifically, we examine one nurse's supposition that expert knowledge is automatically transferable despite having a different role expectation. We share the unexpectedly powerful and potentially ego-deflating experience of one advanced practice nurse (APN) student preparing for the challenge of transitioning from novice to expert as a new APN. However, the greater lesson learned was failing to appreciate the transition in role identity from expert to novice that one undergoes as a registered nurse (RN) to APN student.

  17. Parcels and Land Ownership, Includes APN only, Published in 2007, Churchill County, NV.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Published Reports/Deeds information as of 2007. It is described as 'Includes APN only'....

  18. Favored use of anti-predator netting (APN) applied for the farming of clams leads to little benefits to industry while increasing nearshore impacts and plastics pollution.

    Science.gov (United States)

    Bendell, L I

    2015-02-15

    An overview of the efficacy of anti-predator netting (APN) used by the shellfish industry is presented. There is little support that the currently favored APN effectively protects farmed clams from predators. Evidence does suggest that APN leads to impacts and pollution. APN is an attractant for predators, e.g., crabs, by providing a refuge within Ulva sp. which attaches onto the surface of APN. APN entrains silt and organic matter and increases sediment temperatures degrading habitat underneath the APN. APN present hazards to fish and wildlife and is a source of plastics to the marine environment. The continued use of ineffective APN does not serve either the environment or industry well, and many of these issues could be addressed through the alternate use of "ancient" technology used by aboriginal people to maintain clam gardens; building of rock walls optimizing the amount of clam habitat thereby increasing numbers without the use of APN.

  19. Aminopeptidase N (APN/CD13 inhibitor, Ubenimex, enhances radiation sensitivity in human cervical cancer

    Directory of Open Access Journals (Sweden)

    Nawa Akihiro

    2008-03-01

    Full Text Available Abstract Background Radiotherapy can be used to treat all stages of cervical cancer. For improving local control via radiotherapy, it is important to use additional antitumor agents. Aminopeptidase N (APN/CD13, a 150-kDa metalloproteinase, is a multifunctional cell surface aminopeptidase with ubiquitous expression. Recent studies have suggested that APN/CD13 plays an important role in tumor progression in several human malignancies. Methods We investigated whether the suppression of APN/CD13 using Ubenimex, an inhibitor of APN/CD13 activity, may affect tumor radiosensitivity in cervical cancer cells both in vitro and in vivo. Cell surface APN/CD13 activity in HeLa cells was calculated using alanine-p-nitroanilido as a substrate. For colony formation assays, single-dose radiation and/or Ubenimex were administered to each dish of HeLa cells, and these dishes were cultured for 14 days. Molecular changes of apoptosis were determined by Western blot. Apoptosis was evaluated by Annexin-V PI staining (flow cytometry analysis and the Tunel method. Moreover, we investigated the effect of combining Ubenimex and low-dose radiation on tumor growth using nude mice. Results We demonstrated that Ubenimex enhanced the effectiveness of radiotherapy, acting as a radiosensitizer both in vitro and in vivo. In colony formation assays, a significant decline in clonogenic survival was observed in Ubenimex-treated cells. Mice treated with a combination of radiation and Ubenimex showed a significant prolongation of the tumor-doubling time compared with the control, Ubenimex, or radiation-alone groups. We also showed that ubenimex enhanced radiation-induced apoptosis in vitro and in vivo. Conclusion Although further studies are needed, this report suggests that Ubeniemx acts as a radiosensitizer in cervical cancer treatment, and that the inhibition of APN/CD13 activity may represent a new approach for improving the therapeutic efficacy of radiotherapy for uterine

  20. Reduced activity of CD13/aminopeptidase N (APN) in aggressive meningiomas is associated with increased levels of SPARC.

    Science.gov (United States)

    Mawrin, Christian; Wolke, Carmen; Haase, Daniela; Krüger, Sabine; Firsching, Raimund; Keilhoff, Gerburg; Paulus, Werner; Gutmann, David H; Lal, Anita; Lendeckel, Uwe

    2010-01-01

    Meningiomas are the second most common brain tumors in adults, and meningiomas exhibit a tendency to invade adjacent structures. Compared with high-grade gliomas, little is known about the molecular changes that potentially underlie the invasive behavior of meningiomas. In this study, we examined the expression and function of the membrane alanyl-aminopeptidase [mAAP, aminopeptidase N (APN), CD13, EC3.4.11.2] zinc-dependent ectopeptidase in meningiomas and meningioma cell lines, based on its prior association with tumor invasion in colorectal and renal carcinomas. We found a significant reduction of APNmRNA and protein expression, as well as enzymatic activity, in high-grade meningiomas. While meningioma tumor cell proliferation was not affected by either pharmacologic APN inhibition or siRNA-mediated APN silencing, APN pharmacologic and siRNA knockdown significantly reduced meningioma cell invasion in vitro. Next, we employed pathway-specific cDNA microarray analyses to identify extracellular matrix and adhesion molecules regulated by APN, and found that APN-siRNA knockdown substantially increased the expression of secreted protein, acidic and rich in cysteine (SPARC)/osteonectin. Finally, we demonstrated that SPARC, which has been previously associated with meningioma invasiveness, was increased in aggressive meningiomas. Collectively, these results suggest that APN expression and enzymatic function is reduced in aggressive meningiomas, and that alterations in the balance between APN and SPARC might favor meningioma invasion.

  1. SISTEM PENGENDALIAN TEMPERATUR PADA DINDING BIOREAKTOR ANAEROB SECARA REAL TIME

    OpenAIRE

    Ika Nurina Rachmawati; Rony Dwi Noriyati; Totok Soehartanto

    2013-01-01

    Temperatur merupakan salah satu faktor yang mempengaruhi proses anaerob pada bioreaktor. Dimana pertumbuhan mikroorganisme dipengaruhi oleh perubahan suhu. Maka dari itu akan dilakukan pengendalian temperatur secara real-time pada dinding tabung bioreaktor anaerob. Pengendalian temperatur berguna untuk menjaga suhu permukaan bioreaktor saat terjadi perubahan cuaca dari luar. Sebab jika temperatur dinding dijaga pada suhu 35 0C maka temperatur dalam tabung bioreaktor akan berada pada range ope...

  2. Pengolahan Limbah Cair Industri Fatty Alcohol Secara Anaerobik

    OpenAIRE

    Maya Sarah

    2008-01-01

    Pembuatan fatty alcohol melalui proses transesterifikasi dan hidrogenasi menghasilkan produk fatty alcohol, metil ester, gliserin dan glycerin pitch sebagai limbah. Glycein pitch merupakan cairan kental menyerupai pasta berwarna gelap kecoklatan dengan kandungan COD sebesar 1,8-2: juta mg/l, Pembuangan glycerine pitch menjadi problematika tersendiri yang harus ditangani secara serius bila dikaitkan dengan kecenderungan peningkatan pemanfaatan biodiesel dari metil ester di masa yang akan datan...

  3. Functional interpretation of a non-gut hemocoelic tissue aminopeptidase N (APN in a lepidopteran insect pest Achaea janata.

    Directory of Open Access Journals (Sweden)

    Thuirei Jacob Ningshen

    Full Text Available Insect midgut membrane-anchored aminopeptidases N (APNs are Zn(++ dependent metalloproteases. Their primary role in dietary protein digestion and also as receptors in Cry toxin-induced pathogenesis is well documented. APN expression in few non-gut hemocoelic tissues of lepidopteran insects has also been reported but their functions are widely unknown. In the present study, we observed specific in vitro interaction of Cry1Aa toxin with a 113 kDa AjAPN1 membrane protein of larval fat body, Malpighian tubule and salivary gland of Achaea janata. Analyses of 3D molecular structure of AjAPN1, the predominantly expressed APN isoform in these non-gut hemocoelic tissues of A. janata showed high structural similarity to the Cry1Aa toxin binding midgut APN of Bombyx mori, especially in the toxin binding region. Structural similarity was further substantiated by in vitro binding of Cry1Aa toxin. RNA interference (RNAi resulted in significant down-regulation of AjAPN1 transcript and protein expression in fat body and Malpighian tubule but not in salivary gland. Consequently, reduced AjAPN1 expression resulted in larval mortality, larval growth arrest, development of lethal larval-pupal intermediates, development of smaller pupae and emergence of viable defective adults. In vitro Cry1Aa toxin binding analysis of non-gut hemocoelic tissues of AjAPN1 knockdown larvae showed reduced interaction of Cry1Aa toxin with the 113 kDa AjAPN1 protein, correlating well with the significant silencing of AjAPN1 expression. Thus, our observations suggest AjAPN1 expression in non-gut hemocoelic tissues to play important physiological role(s during post-embryonic development of A. janata. Though specific interaction of Cry1Aa toxin with AjAPN1 of non-gut hemocoelic tissues of A. janata was demonstrated, evidences to prove its functional role as a Cry1Aa toxin receptor will require more in-depth investigation.

  4. Parcels and Land Ownership, Export AS400 to txt join apn, Published in 2007, McKenzie County Government.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset as of 2007. It is described as 'Export AS400 to txt join apn'. The extent of these data is generally Mckenzie County, ND....

  5. Narcolefsia e apnéia do sono concomitantes: registro de caso

    Directory of Open Access Journals (Sweden)

    Rubens Reimão

    1986-03-01

    Full Text Available Relata-se um caso de concomitância de narcolepsia e apnéia do sono tipo obstrutiva documentado clinicamente e em traçados polissonográficos em laboratório de sono. Após correção cirúrgica da apnéia do sono, com traqueostomia, houve manutenção da sintomatologia narcoléptica e de seu registro característico. Este estudo apoia a necessidade de emprego sistemático da polissonografia de noite inteira e do teste de latência múltipla do sono na avaliação de pacientes com sonolência excessiva diurna.

  6. On a Class of Quadratic Polynomials with no Zeros and its Application to APN Functions

    CERN Document Server

    Bracken, Carl; Yin, Tan

    2011-01-01

    We show that the there exists an infinite family of APN functions of the form $F(x)=x^{2^{s}+1} + x^{2^{k+s}+2^k} + cx^{2^{k+s}+1} + c^{2^k}x^{2^k + 2^s} + \\delta x^{2^{k}+1}$, over $\\gf_{2^{2k}}$, where $k$ is an even integer and $\\gcd(2k,s)=1, 3\

  7. Bounds on the degree of APN polynomials: the case of x −1 + g(x)

    DEFF Research Database (Denmark)

    Leander, Gregor; Rodier, François

    2011-01-01

    In this paper we consider APN functions $${f:\\mathcal{F}_{2^m}\\to \\mathcal{F}_{2^m}}$$ of the form f(x) = x −1 + g(x) where g is any non $${\\mathcal{F}_{2}}$$-affine polynomial. We prove a lower bound on the degree of the polynomial g. This bound in particular implies that such a function f is AP...

  8. PEMANFAATAN LIMBAH PENGOLAHAN BUAH MERAH PADA SISTEM PEMELIHARAAN BABI SECARA PASTURA

    OpenAIRE

    Diana Sawen; Bernaddeta Wahyuni Irianti Rahayu; Iriani Sumpe

    2015-01-01

    Suatu penelitian telah dilakukan pada bulan April 2013, untuk mengetahui pemanfaatan limbah hasil pengolahan buah merah pada sistem pemeliharaan ternak babi secara pastura di Kampung Umpakalo Jayawijaya. Bagi masyarakat suku Dani di Lembah Baliem Kabupaten Jayawijaya, babi memiliki nilai penting secara sosial budaya dan ekonomi. Babi tidak dapat dipisahkan dari kehidupan keseharian mereka secara turun temurun. Penelitian menggunakan metode eksperimen dengan percobaan lapangan untuk mengukur p...

  9. The long N-terminus of the C. elegans DNA repair enzyme APN-1 targets the protein to the nucleus of a heterologous system.

    Science.gov (United States)

    Wang, Zhiqiang; Yang, Xiaoming; Mazouzi, Abdelghani; Ramotar, Dindial

    2014-12-15

    We previously isolated from a Caenorhabditis elegans cDNA library, designed for two-hybrid screening, a gene encoding the DNA repair enzyme APN-1 using cross-specie complementation analysis of the Saccharomyces cerevisiae apn1∆ apn2∆ tpp1∆ triple mutant deficient in the ability to repair several types of DNA lesions including apurinic/apyrimidinic (AP) sites. We subsequently purified the APN-1 from this yeast mutant and demonstrated that it possesses four distinct DNA repair activities. However, following the re-annotation of the C. elegans genome we discovered that the functionally active APN-1 encoded by the cDNA from the library might lack 108 amino acid residues from the N-terminus. We therefore synthesized the entire C. elegans apn-1 gene encoding the putative full-length APN-1 and created several N-terminal deletion mutants lacking either 63, 83 or 118 amino acid residues. The full-length APN-1, APN-1 (1-63Δ) and APN-1 (1-83Δ), but not APN-1 (1-118Δ) were stably expressed in the yeast triple mutant and cleaved the AP site substrate. However, only the full-length APN-1 rescued the yeast mutant from the genotoxicity caused by methyl methane sulfonate, a DNA damaging agent that creates AP sites in the genome. The full-length APN-1 was localized to the yeast nucleus, while APN-1 (1-63Δ) and APN-1 (1-83Δ) retained a cytoplasmic distribution. Our data suggest that the N-terminal region has no direct role in the DNA repair functions of APN-1 other than to target the protein to the nucleus and possibly to maintain its stability. Thus, the truncated APN-1, previously isolated from the two-hybrid library, ability to complement the yeast triple mutant depends on the engineered SV40 nuclear localization signal. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Purification of Aminopeptidase N Protein and Differences in cDNAs Encoding APN1 Between Susceptible and Resistant Helicoverpa armigera Strains to Bacillus thuringiensis Toxins

    Institute of Scientific and Technical Information of China (English)

    LIANG Ge-mei; WANG Gui-rong; XU Guang; WU Kong-ming; GUO Yu-yuan

    2004-01-01

    The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera were successfully separated, and most of the Aminopeptidase N (APN) activities in BBMV were preserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-l-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C (PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified using a Mono-Q column. The results of immunoblotting showed that the 120 and 170 kDa proteins in the BBMV could bind CrylAc, and 120kDa APN was a glycosylphosphalidylinositol(GPI)anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after being selected for more than five years in laboratory using Bt insecticides and Bt transgenic cotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1 083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut of susceptible and resistant H.armigera were cloned by PCR and RACE techniques. The inferred amino acid sequences of APN1 possessed the common character of APN family in insects. In comparison with APN1 in susceptible strain, three nucleotide mutations were observed in the APN1 of Bt-M strain and resulted in two amino acid replace in the putative protein sequences, and eight nucleotide mutations were observed in Bt-P strain and resulted in five amino acid replace.

  11. Radioecologycal study of 239/240Pu in Bangka Island and Muria Peninsula: Determination of 239/240Pu in marine sediment and seawater as part of baseline data collecting for sitting of candidates of first Indonesia NPP

    Science.gov (United States)

    Suseno, Heny; Wisnubroto, Djarot S.

    2014-03-01

    Radioisotope Pu-239/240 are alpha emitting nuclides important indicators of radioactive contamination of the marine environment. Global fallout is the main source of plutonium in the marine environment. There are very limited study on 239/240Pu in Indonesia coastal environments. The data of this radioisotopes is needed for baseline data of nuclear power plant (NPP) site candidates both in Bangka Island and Muria Peninsula. Bottom sediments play an important role in radioecological studies of the marine environment because a large proportion of radioactive substances entering the sea is adsorbed over time onto suspended particulate matter and deposited in sediments. Plutonium is particle reactive and deposited in marine sediment. Radioisotope 239/240Pu was determinated by alpha spectrometry after radiochemical procedure that was performed in both water and marine sediment from Bangka Island and Muria Peninsula. The sediment baseline of concentration 239/240Pu in Bangka Island and Muria Peninsula were range from 0.013 to 0.021 Bq.kg-1 and 0.018 to 0.024 Bq.kg-1 respectively. The water baseline concentration this isotope were range from 2.73 to 4.05 mBq.m-3 and 2.98 to 4.50 mBq.m-3.

  12. Radioecologycal study of {sup 239/240}Pu in Bangka Island and Muria Peninsula: Determination of {sup 239/240}Pu in marine sediment and seawater as part of baseline data collecting for sitting of candidates of first Indonesia NPP

    Energy Technology Data Exchange (ETDEWEB)

    Suseno, Heny, E-mail: henis@batan.go.id [Radioactive Waste Technology Center - The Indonesian National Nuclear Energy Agency (Indonesia); Wisnubroto, Djarot S. [The Indonesian National Nuclear Energy Agency (Indonesia)

    2014-03-24

    Radioisotope Pu-239/240 are alpha emitting nuclides important indicators of radioactive contamination of the marine environment. Global fallout is the main source of plutonium in the marine environment. There are very limited study on {sup 239/240}Pu in Indonesia coastal environments. The data of this radioisotopes is needed for baseline data of nuclear power plant (NPP) site candidates both in Bangka Island and Muria Peninsula. Bottom sediments play an important role in radioecological studies of the marine environment because a large proportion of radioactive substances entering the sea is adsorbed over time onto suspended particulate matter and deposited in sediments. Plutonium is particle reactive and deposited in marine sediment. Radioisotope {sup 239/240}Pu was determinated by alpha spectrometry after radiochemical procedure that was performed in both water and marine sediment from Bangka Island and Muria Peninsula. The sediment baseline of concentration {sup 239/240}Pu in Bangka Island and Muria Peninsula were range from 0.013 to 0.021 Bq.kg{sup −1} and 0.018 to 0.024 Bq.kg{sup −1} respectively. The water baseline concentration this isotope were range from 2.73 to 4.05 mBq.m{sup −3} and 2.98 to 4.50 mBq.m{sup −3}.

  13. Perawatan Pulpektomi Secara Singkat Pada Gigi Sulung Nekrotik (Laporan Kasus

    Directory of Open Access Journals (Sweden)

    Sarworini Bagio Budiardjo

    2015-10-01

    Full Text Available Perawatan pulpektomi gigi sulung nekrotik akibat atrisi, trauma atau karies yakni pembuangan seluruh jaringan pulpa nekrotik dan menggantikannya dengan bahan pengisi yang padat, dapat diresorbsi serta tampak secara radiografis. Tujuannya antara lain untuk mempertahankan gigi sulung sampai saatnya digantikan dengan gigi tetap pengganti. Perkembangan, anatomi dan histologi gigi sulung membedakan teknik perawatan pulpektomi gigi sulung dan gigi tetap.Indikasinya adalah gigi sulung nekrotik tanpa peradangan periapikal, mahkota mendukung sterilisasi dan dapat direstorasi serta resorbsi akar fisiologis kurang dari sepertiga akar.Kesulitan perawatan terutama karena bentuk saluran akar yang sangat bervariasi dan resorbsi akar yang tidak beraturan. Tahap perawatan terdiri dari persiapan gigi, sterilisasi, pengisian dan restorasi.Keempat tahap dapat diselesaikan dalam satu kali kunjungan.Pada keadaan dimana terjadi kesulitan persiapan gigi, maka tindakan pengisian saluran akar dan restorasi ditunda 1 minggu kemudian, sehingga perawatan pulpektomi diselesaikan dalam 2 kali kunjungan.Dalam laporan kasus, perawatan pulpektomi dilakukan pada gigi insisif dan molar sulung dengan teknik 1-2 kali kunjungan.Sebagai bahan sterilisasi saluran akar digunakan formokresol dan bahan pengisi campuran zinkoksid-eugenol.  

  14. Pembuatan Biodiesel Secara Batch Dengan Memanfaatkan Gelombang Mikro (Microwave

    Directory of Open Access Journals (Sweden)

    Rhesa Purnama Putra

    2012-09-01

    Full Text Available Biodiesel merupakan bioenergi atau bahan bakar nabati yang dibuat dari minyak nabati, baik minyak baru atau bekas penggorengan melalui proses transesterifikasi, esterifikasi, atau proses esterifikasi-transesterifikasi. Tujuan penelitian ini adalah untuk membuat biodiesel dari minyak goreng secara batch melalui proses transesterifikasi dengan menggunakan radiasi mikrowave serta mempelajari berapa daya dan waktu optimal yang diperlukan untuk proses pembuatan biodiesel dengan radiasi microwave dengan katalis CaO, H2SO4, dan tanpa katalis. Dalam penelitian ini digunakan tiga variabel, yaitu daya (Watt;100,200,300,400, waktu (menit; 5,10,15,20 dan jenis katalis; CaO, H2SO4, dan tanpa katalis. Pada tahap persiapan menghitung volume minyak dan metanol yang akan dicampur. Kemudian mencampurnya didalam reaktor. Lalu mendinginkan campuran hingga terbentuk dua lapisan atas dan bawah dilanjutkan dengan melakukan pemisahan lapisan atas (biodiesel dari lapisan bawah (gliserol. Adapun pada tahap analisis, biodiesel hasil reaksi transesterfikasi dianalisa untuk mendapatkan data yield metil ester, densitas, viskositas, dan flash point. Dari penelitian diketahui bahwa hasil yang didapatkan masih belum dapat memenuhi standar biodiesel yang ditetapkan. Katalis CaO dapat memberikan hasil yang lebih baik dibandingkan dengan katalis H2SO4 dan tanpa katalis. Kondisi operasi untuk menghasilkan kualitas yield biodiesel terbaik yaitu pada daya 200 Watt selama 20 menit dengan menggunakan katalis CaO. Yield biodiesel terbesar didapatkan yield sebesar 60,11 %.

  15. SISTEM PENGENDALIAN TEMPERATUR PADA DINDING BIOREAKTOR ANAEROB SECARA REAL TIME

    Directory of Open Access Journals (Sweden)

    Ika Nurina Rachmawati

    2013-03-01

    Full Text Available Temperatur merupakan salah satu faktor yang mempengaruhi proses anaerob pada bioreaktor. Dimana pertumbuhan mikroorganisme dipengaruhi oleh perubahan suhu. Maka dari itu akan dilakukan pengendalian temperatur secara real-time pada dinding tabung bioreaktor anaerob. Pengendalian temperatur berguna untuk menjaga suhu permukaan bioreaktor saat terjadi perubahan cuaca dari luar. Sebab jika temperatur dinding dijaga pada suhu 35 0C maka temperatur dalam tabung bioreaktor akan berada pada range operasionalnya. Sistem pengendalian temperatur pada dinding bioreaktor berupa elemen pemanas atau heater yang diletakkan pada dinding bioreaktor. Pengukuran temperatur dilakukan pada 3 posisi yaitu temperatur luar, temperature dinding dan temperatur dalam tabung bioreaktor. Pengujian sistem pengendalian dilakukan dengan memberi gangguan pada dinding bioreaktor berupa aliran air dingin pada dinding bioreaktor ketika kondisi telah stabil dengan mengamati pHnya. Pemberian gangguan dilakukan selama beberapa saat hingga terjadi penurunan temperatur di dalam tabung bioreaktor. Dengan turunnya temperatur maka kontroler akan bekerja untuk mencapai temperatur yang diinginkan sesuai dengan setpoint yang diberikan. Hasil pengujian menunjukkan bahwa sistem pengendalian temperatur berhasil menjaga suhu dinding bioreaktor anaerob pada suhu 35 0C dari temperatur 25 0C dalam waktu 1900 detik.

  16. APN连续性排班的实践与成效%Practice and effect of APN continuous scheduling

    Institute of Scientific and Technical Information of China (English)

    罗颖; 林菊芳

    2011-01-01

    目的:探讨实施APN连续性排班的实践与成效.方法:2009年9月将护士排班方式由传统排班模式改为APN连续性排班模式.比较两种排班方式病人对护士工作满意度、健康教育满意率及护士对APN排班的支持率.结果:APN连续性排班模式实施后病人对护士工作满意度、健康教育满意率及护士对分层级管理和APN连续性排班的支持率明显提高,前后比较,均P<0.01,差异具有统计学意义.结论:APN连续性排班和护士分层级管理体现以"病人为中心"的管理理念,充分发挥各层次护理人员的作用,有利于保证护理质量.%Objective To explore the practice and effectiveness of APN coninuity scheduling in nurses. Method8 In September 2009, the traditional shifts were transformed into APN continuity acheduling. Patients' satiafaction on nurses' work and health education and nurses'aatiafaction on the APN acheduling were compared. Results After the implementation of APN continuity scheduling. patients' satisfaction on nursea and health education and nurses' satisfaction on APN and hierarchical management were improved, and the difference was statistically significant (all P < 0.01). Conclusion APN continuity scheduling and nuraes hierarchical management retlects patient-centered management philosophy, pves full play to the role of nunes at all levels, and guarantees the nursinS quality.

  17. The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs

    Directory of Open Access Journals (Sweden)

    Hongcheng Hu

    2015-10-01

    Full Text Available A plastic and biodegradable bone substitute consists of poly (l-lactic-co-glycolic acid and 30 wt % β-tricalcium phosphate has been previously fabricated, but its osteogenic capability required further improvement. We investigated the use of globular adiponectin (gAPN as an anabolic agent for tissue-engineered bone using this scaffold. A qualitative analysis of the bone regeneration process was carried out using μCT and histological analysis 12 weeks after implantation. CBCT (Cone Beam Computed Tomography superimposition was used to characterise the effect of the different treatments on bone formation. In this study, we also explored adiponectin’s (APN influence on primary cultured human jaw bone marrow mesenchymal stem cells gene expressions involved in the osteogenesis. We found that composite scaffolds loaded with gAPN or bone morphogenetic protein 2 (BMP2 exhibited significantly increased bone formation and mineralisation following 12 weeks in the extraction sockets of beagle dogs, as well as enhanced expression of osteogenic markers. In vitro investigation revealed that APN also promoted osteoblast differentiation of primary cultured human jaw bone marrow mesenchymal stem cells (h-JBMMSCs, accompanied by increased activity of alkaline phosphatase, greater mineralisation, and production of the osteoblast-differentiated genes osteocalcin, bone sialoprotein and collagen type I, which was reversed by APPL1 siRNA. Therefore, the composite scaffold loaded with APN exhibited superior activity for guided bone regeneration compared with blank control or Bio-Oss® (a commercially available product. The composite scaffold with APN has significant potential for clinical applications in bone tissue engineering.

  18. Effect of fluvastatin on inflammatory factors, MMP-9, mAlb, Hcy and APN in patients with early diabetic nephropathy

    Institute of Scientific and Technical Information of China (English)

    Peng Ye; Dan Li; Li Chen; Xing Chen

    2016-01-01

    Objective:To investigate the effect of fluvastatin on inflammatory factors, MMP-9, mAlb, Hcy and APN in patients with early diabetic nephropathy.Methods:A total of 80 patients with early diabetic nephropathy were randomly divided into diet group (n=40) and fluvastatin group (n=40), and 40 healthy people were regarded as control group. The two groups both received low protein diet, and the fluvastatin group was additionally given fluvastatin. The treatment time of the two groups was 2 months. The levels of inflammatory factors, MMP-9, mAlb, Hcy and APN in the three groups before treatment, after treatment for 1 month, after treatment for 2 months were detected and compared, respectively.Results:Before treatment, the levels of hs-CRP, TNF-α, mAlb, Hcy and MMP-9 in diet and fluvastatin group were significantly higher while the level of APN was significantly lower than that in control group (P0.05), and there was significant difference of all indexes between diet and fluvastatin group (P0.05), while the level of mAlb decreased significantly than that before treatment and after treatment for 1 month in diet group (P<0.05), there were significant differences of the indexes (hs-CRP, TNF-α, MMP-9, Hcy and APN) between diet and fluvastatin group (P<0.05).Conclusions: Early diabetic nephropathy can lead to abnormal the levels of inflammatory factors, MMP-9, mAlb, Hcy and APN, fluvastatin can significantly improve inflammatory factors and the levels of MMP-9, mAlb, Hcy and APN in patients with early diabetic nephropathy.

  19. The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs.

    Science.gov (United States)

    Hu, Hongcheng; Pu, Yinfei; Lu, Songhe; Zhang, Kuo; Guo, Yuan; Lu, Hui; Li, Deli; Li, Xuefen; Li, Zichen; Wu, Yuwei; Tang, Zhihui

    2015-10-20

    A plastic and biodegradable bone substitute consists of poly (L-lactic-co-glycolic) acid and 30 wt % β-tricalcium phosphate has been previously fabricated, but its osteogenic capability required further improvement. We investigated the use of globular adiponectin (gAPN) as an anabolic agent for tissue-engineered bone using this scaffold. A qualitative analysis of the bone regeneration process was carried out using μCT and histological analysis 12 weeks after implantation. CBCT (Cone Beam Computed Tomography) superimposition was used to characterise the effect of the different treatments on bone formation. In this study, we also explored adiponectin's (APN) influence on primary cultured human jaw bone marrow mesenchymal stem cells gene expressions involved in the osteogenesis. We found OPEN ACCESS Int. J. Mol. Sci. 2015, 16 24947 that composite scaffolds loaded with gAPN or bone morphogenetic protein 2 (BMP2) exhibited significantly increased bone formation and mineralisation following 12 weeks in the extraction sockets of beagle dogs, as well as enhanced expression of osteogenic markers. In vitro investigation revealed that APN also promoted osteoblast differentiation of primary cultured human jaw bone marrow mesenchymal stem cells (h-JBMMSCs), accompanied by increased activity of alkaline phosphatase, greater mineralisation, and production of the osteoblast-differentiated genes osteocalcin, bone sialoprotein and collagen type I, which was reversed by APPL1 siRNA. Therefore, the composite scaffold loaded with APN exhibited superior activity for guided bone regeneration compared with blank control or Bio-Oss® (a commercially available product). The composite scaffold with APN has significant potential for clinical applications in bone tissue engineering.

  20. Papel da gastroplastia no tratamento da apnéia obstrutiva durante o sono

    Directory of Open Access Journals (Sweden)

    Rubens Reimão

    1986-03-01

    Full Text Available Gastroplastia foi realizada como forma terapêutica auxiliar para redução de peso em 4 pacientes com obesidade severa e apnéia obstrutiva durante o sono (ASO. As idades variavam de 31 a 54 anos e todos eram do sexo masculino. Em três casos a gastroplastia acompanhou-se de traqueostomia. Após a gastroplastia todos tiveram melhora da sonolência diurna e redução de peso em 16,7% a 40,9%. Avaliações polissonográficas de noite inteira realizadas no pré e pós-operatório (3-4 meses. Os registros pós-operatórios foram feitos com traqueostomia fechada e revelaram redução da freqüência das apnéias e aumento dos estágios 3, 4 e REM. Normalização dos índices de saturação arterial de oxigênio (Sa0(2 foi constatada em três dos 4 casos. Esses dados sugerem que a gastroplastia pode ser utilizada como forma alternativa para redução de peso em casos selecionados de ASO complementando outros procedimentos cirúrgicos como a traqueostomia.

  1. Factors affecting fluid resuscitation in the burn patient: the collaborative role of the APN.

    Science.gov (United States)

    Zaletel, Cynthia L

    2009-01-01

    Throughout the first critical 24 hr after the injury is sustained, the burn patient must receive fluid resuscitation to prevent hypovolemia and ensure adequate tissue perfusion. Delayed or inadequate fluid resuscitation results in suboptimal tissue perfusion, which can lead to multisystem organ failure and death. Overresuscitation can be more problematic than underresuscitation and has been associated with the development of abdominal compartment syndrome, compartment syndrome of the extremities, airway obstruction, and pulmonary edema. The term fluid creep is used to describe the tendency to give too much fluid and can result from the hemodynamic consequence of opioid creep. Experts in the field agree that fluid resuscitation of the burn patient is a priority. Factors affecting fluid resuscitation in the burn patient are at the cornerstone of burn management. The advanced practice nurse (APN) can play a vital role in implementing strategies to ensure optimal resuscitation in the burn patient. Through collaboration, the APN in both the burn center and the emergency department must make certain that the educational needs of the staff are addressed and be diligent in providing significant education, essential tools, and assistance to the staff nurses in an effort to promote best-practice and evidence-based care.

  2. Climate and Hydrological Data Analysis for hydrological and solute transport modelling purposes in the Muriaé River basin, Atlantic Forest Biome, SE Brazil

    Science.gov (United States)

    Santos, Juliana; Künne, Annika; Kralisch, Sven; Fink, Manfred; Brenning, Alexander

    2016-04-01

    The Muriaé River basin in SE Brazil has been experiencing an increasing pressure on water resources, due to the population growth of the Rio de Janeiro urban area connected with the growth of the industrial and agricultural sector. This leads to water scarcity, riverine forest degradation, soil erosion and water quality problems among other impacts. Additionally the region has been suffering with seasonal precipitation variations leading to extreme events such as droughts, floods and landslides. Climate projections for the near future indicate a high inter-annual variability of rainfall with an increase in the frequency and intensity of heavy rainfall events combined with a statistically significant increase in the duration of dry periods and a reduced duration of wet periods. This may lead to increased soil erosion during the wet season, while the longer dry periods may reduce the vegetation cover, leaving the soil even more exposed and vulnerable to soil erosion. In consequence, it is crucial to understand how climate affects the interaction between the timing of extreme rainfall events, hydrological processes, vegetation growth, soil cover and soil erosion. In this context, physically-based hydrological modelling can contribute to a better understanding of spatial-temporal process dynamics in the Earth's system and support Integrated Water Resourses Management (IWRM) and adaptation strategies. The study area is the Muriaé river basin which has an area of approx. 8000 km² in Minas Gerais and Rio de Janeiro States. The basin is representative of a region of domain of hillslopes areas with the predominancy of pasture for livestock production. This study will present some of the relevant analyses which have been carried out on data (climate and streamflow) prior to using them for hydrological modelling, including consistency checks, homogeneity, pattern and statistical analyses, or annual and seasonal trends detection. Several inconsistencies on the raw data were

  3. Identifikasi Pewarna Sintetis Dalam Minuman Ringan Siena Okabe Strawberry Secara Kromatografi Kertas

    OpenAIRE

    2011-01-01

    Minuman ringan siena okabe strawberry mengandung pewarna sintetis carmoisine yang tidak berbahaya bagi tubuh apabila dikonsumsi pada batas tertentu. Zat pewarna sintesis pada sampel minuman ringan siena okabe strawberry diuji secara kromatografi kertas dengan larutan baku pembanding. Dari hasil identifikasi sampel minuman ringan siena okabe strawberry secara kromatografi kertas diperoleh tinggi bercak dari sampel minuman ringan siena okabe strawberry 7,1 dengan harga Rf 0,591.Dari data terseb...

  4. Cable TV: Bringing Home Native Speaker to Increase Listening Comprehension of the Students of English Education Department Teacher Training and Education Faculty Muria Kudus University

    Directory of Open Access Journals (Sweden)

    Rismiyanto Nuraeningsih

    2016-07-01

    Full Text Available This study investigated the use of cable TV to increase listening comprehension of the students of English education department of Muria Kudus University. The aims were to find out: (1 the listening comprehension achievement of the students taught by using cable TV, (2 the students’ response towards the teaching of listening comprehension class by using cable TV, and (3 the students’ difficulties when being involved in the listening class taught by using cable TV are. A classroom action research was conducted with three cycles. The data was collected by using test, observation checklist, & a questionnaire. The subject consisted of 29 students joining Advanced Listening class. The findings show that: (a The listening comprehension achievement of the students taught by using cable TV in cycle I, II, & III is fair, (b The students have enthusiasm and seriousness and motivation in joining the class in all cycles, (c In cycle III the students’ difficulties when being involved in the listening comprehension class taught by using cable TV are more and more decreasing. Keywords: Cable TV, Listening Comprehension

  5. Trans-complementation by human apurinic endonuclease (Ape) of hypersensitivity to DNA damage and spontaneous mutator phenotype in apn1-yeast.

    Science.gov (United States)

    Wilson, D M; Bennett, R A; Marquis, J C; Ansari, P; Demple, B

    1995-01-01

    Abasic (AP) sites in DNA are potentially lethal and mutagenic. 'Class II' AP endonucleases initiate the repair of these and other DNA lesions. In yeast, the predominant enzyme of this type is Apn1, and its elimination sensitizes the cells to killing by simple alkylating agents or oxidants, and raises the rate of spontaneous mutation. We investigated the ability of the major human class II AP endonuclease, Ape, which is structurally unrelated to Apn1, to replace the yeast enzyme in vivo. Confocal immunomicroscopy studies indicate that approximately 25% of the Ape expressed in yeast is present in the nucleus. High-level Ape expression corresponding to approximately 7000 molecules per nucleus, equal to the normal Apn1 copy number, restored resistance to methyl methanesulfonate to near wild-type levels in Apn1-deficient (apn1-) yeast. Ape expression in apn1- yeast provided little protection against H2O2 challenges, consistent with the weak 3'-repair diesterase activity of the human enzyme. Ape expression at approximately 2000 molecules per nucleus reduced the spontaneous mutation rate of apn1- yeast to that seen for wild-type cells. Because Ape has a powerful AP endonuclease but weak 3'-diesterase activity, these findings indicate that endogenously generated AP sites can drive spontaneous mutagenesis. Images PMID:8559661

  6. [Quality of advanced practice nurse counseling in home care settings (APN-BQ): psychometric testing of the instrument].

    Science.gov (United States)

    Petry, Heidi; Suter-Riederer, Susanne; Kerker-Specker, Carmen; Imhof, Lorenz

    2014-12-01

    Hintergrund: Patientenzentrierte und individuell ausgerichtete Angebote, wie die häusliche Beratung durch Pflegeexpertinnen-APN (Advanced Practice Nurses), eignen sich besonders, chronisch kranke alte Menschen in einer möglichst selbstständigen Lebensführung zu unterstützen. Methode: Um die Qualität einer patientenzentrierten Beratung zu evaluieren, wurde ein 23-Item Instrument entwickelt und seine psychometrischen Eigenschaften mit einer Stichprobe von 206 Personen, die 80 Jahre und älter waren getestet. Ziel: Ziel dieses Artikels ist es, die Entwicklung und Evaluation des APN-BQ zu beschreiben. Die psychometrische Testung des Instruments erfolgte anhand einer Hauptkomponentenanalyse mit Varimax-Rotation. Ergebnisse: Die Analyse ergab eine stabile vier Faktorenstruktur (FS = 0,91) mit 19 Items. Alle Faktoren hatten eine Faktorladung > 0,45. Die interne Konsistenz der Gesamtskala ergab einen Wert von Cronbachs alpha 0,86. Die hohe Rücklaufquote der Fragebogen und die Tatsache, dass 98,8 % der Fragen beantwortet wurden, bestätigten die Anwendungsfreundlichkeit und Akzeptanz des Instruments. Schlussfolgerungen: Das APN-BQ erwies sich als zuverlässiges und in Bezug auf Inhalt und Konstrukt valides Instrument, die Struktur-, Prozess- und Ergebnisqualität einer patientenzentrierten Beratungsintervention in der gemeindenahen Versorgung sowie das Ausmaß der Partizipation und Selbstbefähigung (Empowerment) der zu Beratenden zu messen.

  7. PC, a Novel Oral Insecticidal Toxin from Bacillus bombysepticus Involved in Host Lethality via APN and BtR-175.

    Science.gov (United States)

    Lin, Ping; Cheng, Tingcai; Jin, Shengkai; Wu, Yuqian; Fu, Bohua; Long, Renwen; Zhao, Ping; Xia, Qingyou

    2015-06-09

    Insect pests have developed resistance to chemical insecticides, insecticidal toxins as bioinsecticides or genetic protection built into crops. Consequently, novel, orally active insecticidal toxins would be valuable biological alternatives for pest control. Here, we identified a novel insecticidal toxin, parasporal crystal toxin (PC), from Bacillus bombysepticus (Bb). PC shows oral pathogenic activity and lethality towards silkworms and Cry1Ac-resistant Helicoverpa armigera strains. In vitro assays, PC after activated by trypsin binds to BmAPN4 and BtR-175 by interacting with CR7 and CR12 fragments. Additionally, trypsin-activated PC demonstrates cytotoxicity against Sf9 cells expressing BmAPN4, revealing that BmAPN4 serves as a functional receptor that participates in Bb and PC pathogenicity. In vivo assay, knocking out BtR-175 increased the resistance of silkworms to PC. These data suggest that PC is the first protein with insecticidal activity identified in Bb that is capable of causing silkworm death via receptor interactions, representing an important advance in our understanding of the toxicity of Bb and the contributions of interactions between microbial pathogens and insects to disease pathology. Furthermore, the potency of PC as an insecticidal protein makes it a good candidate for inclusion in integrated agricultural pest management systems.

  8. Horta orgânica de ervas medicinais: inclusão social na comunidade da Barra em Muriaé/MG - Brasil

    Directory of Open Access Journals (Sweden)

    José Luiz de Freitas Paixão

    2013-09-01

    Full Text Available Foi implantada uma horta orgânica de ervas medicinais na comunidade da Barra (Muriaé, MG com o objetivo de inclusão social de pessoas carentes ao permitir acesso ao tratamento por fitoterapia. O início das atividades foi em janeiro de 2006, com a escolha das espécies, obtenção de mudas e instalação da horta. As 40 espécies selecionadas, sob manejo agroecológico, eram indicadas com terapêutica aos 25 principais problemas de saúde levantados na comunidade. O formato didático da horta, representando o corpo humano, serviu como referência às indicações de uso de cada espécie. A horta foi mantida até meados de 2012 na Barra e transferida ao IFMURIAÉ, em área de cultivo agroecológico, no Bairro Sofoco, onde se realizam as práticas do curso de técnico em agroecologia. A comunidade da Barra auxiliou desde as primeiras ações, sendo atuante na colheita das plantas medicinais. As plantas coletadas foram levadas a sala anexa à Paróquia local onde se realizou o processamento pós-colheita e o preparo de fitoterápicos. A dispensação dos preparados foi em outra sala cedida pela Paróquia. As formas fitoterápicas disponibilizadas à população foram de uso interno (erva seca, tintura, xarope e externo (creme, gel, pomada, sendo as mais procuradas: gel, tintura e xarope. De 2006 a 2012 foram  32.400 atendimentos, média de 385 pessoas/mês. O sucesso do projeto se reflete no aumento do número de espécies utilizadas, passando de 40 espécies em 2006 para 170 espécies em 2012, dentre elas: alecrim de jardim (Rosmarinus officinalis, alfavaca (Ocimum basilicum, amora (Morus alba, arnica brasileira (Solidago microglossa, calêndula (Calendula officinalis, camomila (Matricaria chamomilla, capim gordura (Melinis minutiflora, cavalinha (Equisetum arvense, chapéu-de-couro (Echinodorus grandiflorus e espinheira santa (Maytenus aquifolium.

  9. Expression of Aminopeptidase N1(APN1),the Main Receptor Protein for Bacillus thuringiensis Cry1A Toxin from Helicoverpa armigera Larval Midgut in Trichoplusia ni cells

    Institute of Scientific and Technical Information of China (English)

    CHANG Hong-lei; LIANG Ge-mei; WANG Gui-rong; YU Hong-kun; GUO Yu-yuan; WU Kong-ming

    2008-01-01

    The aim of this article is to successfully express the Bt(Bacillus thuringiensis)toxin receptor protein located on the internal membrane of larval midgut of cotton bollworm(Helicoverpa armigera Hiibner)within eukaryotic expression system,which is one of the key links for clarifying the relationship between receptor and Bt resistance.The fragments of aminopeptidase N1(APN1)gene without signal peptide in the susceptible and the resistant H. armigera were cloned separately using PCR method,and were separately cloned into pUC 19 vector.After sequencing the gene,the fragments encoding for APN1 without signal peptide were cloned into the Bac-to-Bac baculovirus expression system with transfer vector pFastBacHTB under the polyhedron gene promoter.The recombinant transposing plasmid pFastBacHTB/APN1 was screened and then transformed into Escherichia coli DH10Bac.It was cultured in LB medium,which contained Te, Kan,Ge,X-gal,and IPTG.The resulting recombinant bacmid was transfected into cells of the insect Trichoplusia ni and recombinant baculoviruse was obtained.The lysate of cells infected with recombinant baculoviruse was analyzed by SDS-PAGE and blot analysis.The results showed that the recombinant baculoviruse was fully capable of expressing APN1.The APN1 gene successfully expressed in T. ni cell established the base for continuing the research on its function and relationship of resistance with Bt.

  10. Silencing of CYP6 and APN Genes Affects the Growth and Development of Rice Yellow Stem Borer, Scirpophaga incertulas

    OpenAIRE

    Vijaya Sudhakara Rao eKola; P eRenuka; Ayyagari Phani Padmakumari; Satendra Kumar Mangrauthia; Balachandran eS M; MAGANTI SHESHU MADHAV

    2016-01-01

    RNAi is a powerful tool to target the insect genes involved in host-pest interactions. Key insect genes are the choice for silencing to achieve pest derived resistance where resistance genes are not available in gene pool of host plant. In this study, an attempt was made to determine the effect of dsRNA designed from two genes Cytochrome P450 derivative (CYP6) and Aminopeptidase N (APN) of rice yellow stem borer (YSB) on growth and development of insect. The bioassays involved injection of ch...

  11. PANJANG SIKLUS ESTRUS MENCIT (Mus musculus L. YANG DIBERI PEMANIS BUATAN ASPARTAM SECARA ORAL

    Directory of Open Access Journals (Sweden)

    Sri Sulastri

    2015-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh pemberian aspartam terhadap panjang siklus estrus mencit betina dewasa. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL dengan 4 perlakuan dan 6 ulangan. Perlakuan P0 sebagai kontrol diberi aquades dan perlakuan P1, P2 dan P3 diberi aspartam dosis10 mg/kg bb; 15 mg/kg bb dan20 mg/kg bb. Aspartam diberikan setiap hari secara oral (gavage selama 14 hari sebanyak 0,3 ml. Setelah 14 hari, apusan vagina dibuat setiap 8 jam dalam sehari selama dua kali siklus estrus. Variabel yang diamati adalah panjang waktu tiap fase dalam siklus estrus. Hasil analisis menggunakan Uji One Way Anova dan Uji Kruskal Wallis menunjukkan bahwa aspartam secara nyata (P<0,05 memperpanjang siklus estrus dengan peningkatan dosis yang diberikan.

  12. Penetapan Kadar Cimetidin Pada Omekur Tablet Secara Spektrofotometri Di. PT Mutiara Mukti Farama Medan

    OpenAIRE

    Joshua Alexander G.

    2011-01-01

    Tablet adalah sediaan padat, dibuat secara kempa-cetak berbentuk rata atau cembung rangkap, umumnya bulat, mengandung satu jenis obat atau lebih dengan atau tanpa zat tambahan (Anief, 1999). Obat adalah semua bahan tunggal atau campuran yang digunakan oleh semua makhluk untuk bagian dalam maupun bagian luar, guna mencegah, meringankan, maupun menyembuhkan penyakit. Menurut undang-undang kesehatan, yang dimaksud dengan obat adalah suatu bahan atau campuran bahan yang dimaksudkan untuk digu...

  13. Perubahan Klinik Pada Anjing Lokal Selama Teranestesi Ketamin Dengan Berbagai Dosis Premedikasi Xilazin Secara Subkutan

    Directory of Open Access Journals (Sweden)

    Kadek Mira Dwiningrum

    2016-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui perubahan klinik yang terjadi selama teranestesi ketamin dengan premedikasi xilazin yang melebihi dosis pemberian secara intramuskuler pada anjing lokal yang diberikan secara subkutan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL dengan empat perlakuan yaitu pemberian xilazin dosis 2 mg/kg bb (kontrol, 4 mg/kg bb, 6 mg/kg bb, dan 8 mg/kg bb. Setiap perlakuan menggunakan enam ekor anjing sebagai ulangan, sehingga anjing yang digunakan sebanyak 24 ekor. Data kuantitatif yang diperoleh dianalisis dengan Sidik Ragam dan dilanjutkan dengan Uji Wilayah Berganda Duncan, sedangkan data kualitatif yang diperoleh disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa adanya perubahan klinik pada dosis premedikasi 2 mg/kg bb, 6 mg/kg bb dan 8 mg/kg bb, sedangkan pada dosis 4 mg/kg bb anjing tidak teranestesi sempurna sehingga tidak dilakukan pengamatan perubahan klinik pada dosis 4 mg/kg bb. Perbedaan dosis premedikasi xilazin berpengaruh nyata (P0,05 terhadap pulsus, CRT, frekuensi respirasi, suhu tubuh, dan tekanan otot rahang. Perbedaan waktu pengamatan selama anjing teranestesi berpengaruh sangat nyata (P

  14. ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG

    Directory of Open Access Journals (Sweden)

    Jumiyati -

    2012-03-01

    Full Text Available Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes had been collected from the soil of Biology Educational Garden Semarang State University.

  15. Penerapan Pasal 1320 KUH Perdata terhadap Jual Beli secara Online (E-Commerce

    Directory of Open Access Journals (Sweden)

    Herniwati ,

    2015-07-01

    Full Text Available Pasal 1320 KUHPerdata mengatur tentang syarat-syarat sah perjanjian. Ketentuan Pasal 1320 ini berlaku terhadap perjanjian jual beli secara konvensional dan perjanjian jual beli melalui online (internet Dewasa ini Perjanjian jual beli melalui online sangat diminati oleh kaum muda dan kaum ibu rumah tangga.Transaksi jual beli secara online atau disebut dengan e-commerce yang dilakukan melalui  media elektronik sudah sangat populer. Para pembeli yang akan membeli barang-barang atau jasa yang ditawarkan oleh penjual (pedagang. Dengan perkembangan teknologi informasi ini akan berdampak terhadap perkembangan aturan-aturan hukum yang ada. Atauran hukum yang diatur dalam KUHPerdata mengatur tentang  syarat-syarat perjanjian. Pasal 1320 KUHPerdata mengatur tentang syarat-syarat sah perjanjian. Ketentuan ini juga berlaku terhadap transaksi jual beli secara online. Ketentuan dalam KUHPerdata ini diperkuat  dengan adanya  Undang-Undang Nomor  11 Tahun 2008 Tentang Informasi dan Transaksi  Elektronik yang mengatur tentang transaksi elektronik.Kata Kunci:  Jual Beli, e-commerce

  16. PEMANFAATAN LIMBAH PENGOLAHAN BUAH MERAH PADA SISTEM PEMELIHARAAN BABI SECARA PASTURA

    Directory of Open Access Journals (Sweden)

    Diana Sawen

    2015-06-01

    Full Text Available Suatu penelitian telah dilakukan pada bulan April 2013, untuk mengetahui pemanfaatan limbah hasil pengolahan buah merah pada sistem pemeliharaan ternak babi secara pastura di Kampung Umpakalo Jayawijaya. Bagi masyarakat suku Dani di Lembah Baliem Kabupaten Jayawijaya, babi memiliki nilai penting secara sosial budaya dan ekonomi. Babi tidak dapat dipisahkan dari kehidupan keseharian mereka secara turun temurun. Penelitian menggunakan metode eksperimen dengan percobaan lapangan untuk mengukur pertambahan bobot badan. Eksperimen dilakukan untuk mengetahui pengaruh dari tiga perbedaan pakan tambahan menggunakan limbah buah merah (kontrol, biji buah merah dan biji + sari buah merah. Jumlah sampel ternak sebanyak 15 ekor babi lokal fase grower. Hasil penelitian menunjukkan bahwa adanya kecenderungan peningkatan berat badan pada perlakuan dengan penambahan biji + sari buah merah. Pertambahan bobot badan harian yang diperoleh dari semua perlakuan adalah 0,41 kg. Hal ini karena sari buah merah mengandung betakaroten dan tokoferol yang lebih tinggi sehingga sangat membantu dalam pertumbuhan ternak di samping pakan lainnya yang dikonsumsi. Limbah buah merah sangat potensial untuk dikembangkan sebagai pakan

  17. ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG

    Directory of Open Access Journals (Sweden)

    Jumiyati -

    2012-03-01

    Full Text Available Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes had been collected from the soil of Biology Educational Garden Semarang State University.

  18. [Being cared for and caring: living with multiple chronic diseases (Leila)-a qualitative study about APN contributions to integrated care].

    Science.gov (United States)

    Müller-Staub, Maria; Zigan, Nicole; Händler-Schuster, Daniela; Probst, Sebastian; Monego, Renate; Imhof, Lorenz

    2015-04-01

    Hintergrund: Das Leben mit mehreren chronischen Krankheiten ist komplex und führt zu einer erhöhten Pflege- und Betreuungsbedürftigkeit. Zur Förderung der integrierten Versorgung wurde ein dreijähriges Projekt namens «Leben mit Langzeiterkrankung» (Leila) initiiert. Ziel: Das Projekt hatte zum Ziel, Leistungen von Pflegeexpertinnen in Advanced Practice Nursing (APN) für chronisch erkrankte Menschen in Zusammenarbeit mit Hausarztpraxen zu entwickeln. Folgende Fragestellungen wurden untersucht: 1. Wie erleben Patient(inn)en, Zuweisende und Pflegeexpertinnen APN das Leila-Dienstleistungsangebot? 2. Wie gestalten sich Zuweisungsprozesse? 3. Wie erleben die Beteiligten die Zusammenarbeit und die APN-Rollenausübung? Methode: Es wurde ein qualitativer Ansatz gemäß Grounded Theory nach Corbin und Strauss gewählt, um Erfahrungen mit Leila und Interaktionen der an Leila beteiligten Personen zu untersuchen. Dabei wurden 38 Interviews mit Patient(inn)en, die mit multiplen chronischen Krankheiten leben, deren betreuende Pflegeexpertinnen APN und zuweisenden Ärzt(inn)en durchgeführt und evaluiert. Ergebnisse: «Umsorgt werden und umsorgen» zeigte sich als zentrale Kategorie. Diese beschreibt wie auf die Patient(inn)en eingegangen wurde, sie einbezogen und ernst genommen wurden. Die Hauptkategorie «Den Alltag gestalten» zeigt, dass die Patient(inn)en lernten, mit den Folgen chronischer Erkrankungen im Alltag umzugehen. «Alle Ressourcen nutzen» legt dar, wie Potenziale und Stärken eingesetzt wurden. Schlussfolgerungen: Die Ergebnisse zu Zuweisungs- und Kooperationsprozessen ergaben, dass die APN-Rollenanerkennung und -ausübung noch ausgehandelt werden muss. Zukünftige APN-Angebote für diese Patient(inn)engruppe sollten nebst Ärztenetzwerken in weitere Dienstleistungen und die Spitex integriert werden.

  19. Penetapan Harga PKA Dari Ketoprofen Bpfi Dan Beberapa Sediaan Tablet Ketoprofen Yang Terdapat Dipasaran Dengan Metode Spektrofotometri Ultraviolet Secara Isobestik

    OpenAIRE

    Rina Astuti

    2010-01-01

    Ketoprofen yang terdapat dipasaran diproduksi oleh beberapa industri farmasi dan masing-masing industri memiliki formulasi yang berbeda, dengan perbedaan formulasi ini tentu akan mempengaruhi harga pKa dari molekul obat yang akan menentukan absorpsi dan distribusi obat dalam jaringan-jaringan tubuh. Harga pKa yang diperoleh dari suatu senyawa tergantung dari metode yang digunakan, diantaranya perhitungan secara ? Hammett (Siswandono & Soekardjo, 1998), secara Spektrofotometri Ultraviolet m...

  20. 棉铃虫中肠Bt毒素受体蛋白(APN)的分离与纯化%Isolation and Purification of Aminopeptidase N (APNs), the Main Receptor Proteins for Bacillus thuringiensis toxin in Midgut of Helicoverpa armigera Larva

    Institute of Scientific and Technical Information of China (English)

    梁革梅; 徐广; 王桂荣; 吴孔明; 郭予元

    2005-01-01

    利用Mg/EGTA方法,以不同的速度进行离心,成功地分离了棉铃虫(Bacillus thuringiensis)中肠刷状缘膜囊泡(BBMV0,BBMV中保留了大部分氨肽酶N(aminopeptidase N,APN)活性,BBMV中APN的特异活性较粗匀浆液相比提高了3.29倍;CHAPS3-[(3-chlor-amidopropyl)dimethylammonio]-1-propane sulphonate可以增加BBMV的溶解,加入1%(CHAPS)之后APN特异活性增加至5.21倍;利用磷酸酰肌醇特异性的磷脂酶(PI-PLC)能将APN从膜上解离下来,BBMV经PI-PLC处理后,SDS-PAGE电泳结果仅有120 kD的1条带;Mono-Q和FPLC结合的方法可以纯化部分APN,SDS-PAGE电泳结果显示,经Mono-Q柱处理后的BBMV中分子量约100 kD的蛋白条带加粗;棉铃虫3个抗性品系与敏感品系相比,APN特异活性差异不显著,说明棉铃虫对Bt产生抗性与APN活性的变化无关,抗性的产生可能与Bt毒素和APN结合能力的改变有关.

  1. RANCANG BANGUN APLIKASI PENGAMBILAN BERITA SECARA OTOMATIS MENGGUNAKAN CONTENT SYNDICATION BERBASIS XML DENGAN PLATFORM MICROSOFT .NET

    Directory of Open Access Journals (Sweden)

    Diana Purwitasari

    2004-01-01

    Full Text Available Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Banyaknya kebutuhan akan informasi di internet menyebabkan penyedia jasa situs berita untuk memberikan berita yang selalu yang terbaru. Salah satu alternatif solusi adalah dengan melakukan content syndication. Content syndication adalah adalah proses dimana suatu isi berita dikirimkan atau disediakan, biasanya dengan biaya tertentu, dari penyedia berita, biasanya disebut originators, ke pasar yang membutuhkan atau subscribers. RSS (Rich Site Summary adalah format yang secara umum digunakan untuk melakukannya. RSS pada dasarnya adalah suatu file yang berada di suatu situs, yang menyediakan informasi tentang isi dari situs tersebut. File tersebut biasa disebut sebagai RSS Feeds dan dapat di ambil dan diolah untuk mendapatkan informasi tentang isi situs tersebut. Dibuat sebuah aplikasi untuk pengambilan situs berita secara otomatis menggunakan content syndication yang memerlukan aplikasi pada proses background untuk mengambil RSS Feeds secara berkala pada komputer yang berfungsi sebagai server. Server yang mengambil berita dari situs penyedia terdiri dari aplikasi yang mengatur konfigurasi berita tersebut, dan sebuah windows service untuk mengambil RSS feeds kemudian mengolahnya secara otomatis. Sedangkan aplikasi

  2. Upaya Kodifikasi Hukum Kewarisan secara Bilateral dengan Pola Diferensiasi dalam Masyarakat Pluralis

    Directory of Open Access Journals (Sweden)

    Andi Nuzul

    2012-02-01

    Full Text Available The currently applicable inheritance law in Indonesia is pluralistic in nature since it is formed collectively by adat, Islamic, and western legal systems; not to mention the diverse custom, ethnicity, and belief. Hence, codification of bilateral inheritance law that could address differences in our inheritance and kinship system is needed. Hukum kewarisan yang berlaku di Indonesia saat ini bersifat pluralistis karena disusun bersama-sama oleh hukum adat, Islam, dan Barat serta dilatarbelakangi oleh keanekaragaman adat, etnis, dan agama. Oleh karenanya, diperlukan kodifikasi hukum kewarisan secara bilateral dan bersifat diferensiasi untuk mengakomodasi pluralitas hukum kewarisan dan sistem kekerabatan yang ada di Indonesia.

  3. Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells.

    Science.gov (United States)

    Lefevre, Sophie; Brossas, Caroline; Auchère, Françoise; Boggetto, Nicole; Camadro, Jean-Michel; Santos, Renata

    2012-09-15

    Frataxin deficiency results in mitochondrial dysfunction and oxidative stress and it is the cause of the hereditary neurodegenerative disease Friedreich ataxia (FA). Here, we present evidence that one of the pleiotropic effects of oxidative stress in frataxin-deficient yeast cells (Δyfh1 mutant) is damage to nuclear DNA and that repair requires the Apn1 AP-endonuclease of the base excision repair pathway. Major phenotypes of Δyfh1 cells are respiratory deficit, disturbed iron homeostasis and sensitivity to oxidants. These phenotypes are weak or absent under anaerobiosis. We show here that exposure of anaerobically grown Δyfh1 cells to oxygen leads to down-regulation of antioxidant defenses, increase in reactive oxygen species, delay in G1- and S-phases of the cell cycle and damage to mitochondrial and nuclear DNA. Nuclear DNA lesions in Δyfh1 cells are primarily caused by oxidized bases and single-strand breaks that can be detected 15-30 min after oxygen exposition. The Apn1 enzyme is essential for the repair of the DNA lesions in Δyfh1 cells. Compared with Δyfh1, the double Δyfh1Δapn1 mutant shows growth impairment, increased mutagenesis and extreme sensitivity to H(2)O(2). On the contrary, overexpression of the APN1 gene in Δyfh1 cells decreases spontaneous and induced mutagenesis. Our results show that frataxin deficiency in yeast cells leads to increased DNA base oxidation and requirement of Apn1 for repair, suggesting that DNA damage and repair could be important features in FA disease progression.

  4. Apnéia obstrutiva durante o sono: avaliação clínica e poligráfica de 150 casos

    Directory of Open Access Journals (Sweden)

    Rubens Reimão

    1985-06-01

    Full Text Available Apnéia do sono, tipo obstrutivo, é uma das causas mais frequentes de sonolência excessiva. A anamnese geralmente é sugestiva e, em nossa casuística, caracterizou-se por fadiga ou sonolência excessiva que prejudicava as atividades diárias em 90,6% dos casos e, durante a noite, o sono era tipicamente entrecortado por roncar (92,0% e pausas respiratórias (12,6%. A ocorrência crônica acompanha-se de hipertensão pulmonar e sistêmica, bem como de comprometimento de ritmo e condução cardíacos, potencialmente letais. Por ocasião da procura do atendimento médico, 87,2% dos pacientes tinham entre 40 e 69 anos de idade. A avaliação poligráfica é sempre necessária para o diagnóstico e, nesta casuística, mostrou numerosas apnéias obstrutivas ou mistas, levando ao índice médio de apnéias 37,1; despertares parciais acompanhavam as apnéias; houve superficialização do sono com diminuição de estágios 3 e 4; diminuição da eficiência do sono; hipóxia severa durante as pausas respiratórias; alterações do ECG secundárias às apnéias. O tratamento cirúrgico é o que propicia melhores resultados, a traqueostomia e a uveopalatofaringoplastia sendo as intervenções mais indicadas.

  5. Gambaran Total Eritrosit, Kadar Hemoglobin, Nilai Hematokrit Terhadap Xilazin-Ketamin pada Anjing Lokal secara Subkutan

    Directory of Open Access Journals (Sweden)

    Andra Marshanindya

    2016-06-01

    Full Text Available Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian xilazin denganberbagai dosis dan ketamin secara subkutan terhadap gambaran total eritrosit, kadar hemoglobin, nilaihematokrit. Penelitian ini menggunakan 24 ekor anjing lokal dan pengambilan darah selama anestesidengan selang waktu 20 menit sampai menit ke-100, dilanjutkan dengan pemeriksaan total eritrosit,kadar hemoglobin dan nilai hematokrit. Ketamin diberikan secara IM pada kontrol/perlakuan 1 danSC pada perlakuan 2,3,4. Xilazin diberikan dengan dosis 2 mg/kg pada kontrol (IM, 4 mg/kg padaperlakuan 2 (SC, 6 mg/kg pada perlakuan 3 (SC dan 8 mg/kg pada perlakuan 4 (SC. Hasilpenelitian menunjukkan bahwa terjadi penurunan total eritrosit dan nilai hematokrit anjing selamamasa anestesi dan terjadi peningkatan selama masa pemulihan kesadaran namun masih berada padakisaran normal. Analisis data yang dilakukan menunjukkan bahwa dosis pemberian berpengaruhnyata (P0,05 namun waktu pemeriksaan bepengaruh sangat nyata (P0,05 terhadap total eritrosit dan nilaihematokrit. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa adanya pengaruh terhadap totaleritrosit dan nilai hematokrit sedangkan terhadap kadar hemoglobin perbedaan dosis premedikasitidak berpengaruh. Namun perbedaan waktu pengamatan selama anjing teranestesi hanya berpengaruhterhadap kadar hemoglobin sedangkan pada total eritrosit dan nilai hematokrit tidak berpengaruh.

  6. Pengelolaan Sampah Berbasis “Zero Waste” Skala Rumah Tangga Secara Mandiri

    Directory of Open Access Journals (Sweden)

    Ika Wahyuning Widiarti

    2012-06-01

    Full Text Available Pengelolaan secara zero waste merupakan pengelolaan dengan melakukan pemilahan, pengomposan dan pengumpulan barang layak jual. Pemilahan sampah dalam rumah tangga harus didukung fasilitas pewadahan berupa tong sampah yang memadai. Tong sampah yang harus disediakan dalam rumah cukup dibagi untuk 2 jenis sampah yaitu sampah organik (basah dan sampah anorganik (kering. Sampah yang telah terpilah menjadi sampah basah dan kering selanjutnya dilakukan pengelolaan yaitu pengomposan dan pengumpulan sampah layak jual. Pengomposan merupakan teknik untuk mengolah sampah organik yang berupa sampah sisa makanan, sisa potongan sayur dan buah atau sampah dapur dan sampah dari sapuan halaman rumah. Jika sampah organik rumah tangga dikelola secara mandiri (on site dengan cara dikomposkan maka sampah anorganik harus dikelola dengan bantuan pihak ketiga (off site. Pihak ketiga yang dapat mendukung pengelolaan sampah anorganik rumah tangga adalah para pelaku usaha daur ulang informal antara lain pemulung, tukang loak, pengrajin produk daur ulang khusus untuk sampah plastik kemasan berlapis aluminium foil serta ada alternatif baru yaitu menabung sampah di bank sampah.

  7. PEMBERIAN OBAT SECARA POLIFARMASI PADA ANAK DAN INTERAKSI OBAT YANG DITIMBULKAN

    Directory of Open Access Journals (Sweden)

    Ernie HP

    2012-09-01

    Full Text Available Dalam konsep tumbuh kembang, anak bukanlah miniatur dewasa terutama bila dikaitkan dengan pengobatan. Pengobatan yang diberikan mengacu pada patogenesis dan patofisiologis penyakit, sehingga pemilihan obat ditentukan berdasarkan kausa dan atau gejala penyakit. Akan tetapi, tidak semua gejala yang dikeluhkan penderita akan diobati. Dengan demikian, jumlah obat yang diberikan tidak berlebihan. Pemberian dua macam obat atau lebih dapat menimbulkan interaksi obat. Berdasarkan hasil observasi terhadap pendidikan terintegrasi sejak tahun 2000 antara Departemen I. Farmasi Kedokteran dan Departemen I. Kesehatan Anak FKUI, dijumpai masalah yang dapat menimbulkan interaksi obat yang merugikan pada penderita anak-anak karena pemberian obat yang berlebihan, yaitu lebih dari 4 macam obat yang dikenal dengan pengobatan secara polifarmasi. Atas dasar permasalahan tersebut, dilakukan analisis terhadap sejumlah resep untuk penderita anak-anak yang masuk ke Apotik "A " (nama Apotik ada pada Dewan Redaksi di Jakarta Selatan pada tahun 2005. Sebanyak 480 resep yang umumnya ditulis oleh dokter ahli IKA masuk dalam analisis, 53% di antaranya merupakan pemberian obat secara polifarmasi (lebih dari 4 obat dan 12% di antaranya memicu timbulnya interaksi obat yang tidak diinginkan.Kata kunci: polifarmasi, interaksi obat, penyakit anak-anak  

  8. SISTEM INFORMASI PENGELOLAAN PROPOSAL PENELITIAN DAN PENGABDIAN MASYARAKAT SECARA ON-LINE

    Directory of Open Access Journals (Sweden)

    Gede Sukadarmika

    2014-12-01

    Full Text Available Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM Universitas Udayana merupakan salah satu unsur di Universitas Udayana yang berfungsi untuk mengkoordinasikan, memantau dan menilai pelaksanaan dan kegiatan penelitian yang diselenggarakan oleh pusat- pusat penelitian atau pun oleh kelompok-kelompok peneliti dari berbagai fakultas atau program studi. Selain itu, LPPM juga berperan untum memberikan pelayanan bagi para peneliti di lingkungan Universitas Udayana dalam hal penyediaan informasi penelitian, proses administrasi dan juga layanan pembinaan kemampuan para peneliti. Hingga saat ini, LPPM mengelola berbagai jenis hibah penelitian baik yang merupakan program dari DIKTI maupun penelitian yang di kelola di Universitas Udayana. Jumlah proposal penelitian yang dikelola oleh LPPM dari tahun ketahun semakin meningkat seiring dengan semakin meningkatnya gairah para peneliti Universitas Udayana untuk melakukan penelitian pada bidang yang ditekuninya. Jumlah proposal yang dikelola oleh LPPM untuk di kompetisikan pada hibah-hibah yang ada pada tahun 2010 mencapai 765 proposal. Sistem Informasi Pengelolaan Proposal Penelitian diupayakan untuk mengefektifkan kerja dari staf LPPM dan juga memberikan kemudahan bagi peneliti dan reviewer dalam melaksanakan tugasnya. Pada sistem ini, para peneliti meregistrasikan proposalnya secara online. Selanjutnya, staff LPPM melakukan rekap terhadap jumlah proposal pada semua jenis hibah yang ada selanjutnya mendistribusikannya kepada reviewer untuk dinilai. Reviewer memberikan penilaian terhadap proposal yang dikompetisikan secara online. Berdasarkan skor yang diinputkan tersebut, sistem mengolah dan memberikan daftar ranking proposal yang dikompetisikan. Dengan demikian, LPPM diharapkan dapat menjamin akuntabilitas dan transparansi pengelolaan proposal penelitian disamping juga untuk meningkatkan kinerja lembaga.

  9. Ocorrência de complicações crônicas e seus fatores de risco em pacientes diabéticos atendidos pelo Programa Saúde da Família de Muriaé, MG, Brasil

    Directory of Open Access Journals (Sweden)

    Pedro Paulo Pedrosa NETTO

    2015-01-01

    Full Text Available O objetivo deste trabalho foi analisar a ocorrência de complicações associadas ao diabetes e identificar seus fatores de risco em indivíduos com diabetes mellitus assistidos pelo Programa Saúde da Família de Muriaé-MG. Foi realizado um estudo quantitativo, transversal no período de fevereiro a junho de 2009 na unidade do Programa de Saúde da Família (PSF do bairro São Gotardo no município de Muriaé, MG. A amostra foi constituída por 50 pacientes. As informações de cada participante foram obtidas por meio de um questionário estruturado. As informações sobre a ocorrência de complicações crônicas do diabetes foram obtidas por meio da análise dos prontuários dos pacientes. Foi observado que 23 (46,0% pacientes não apresentavam complicação da doença, enquanto 27 (54,0% apresentavam pelo menos uma complicação, sendo as mais frequentes retinopatia, presente em 18 (66,67% pacientes, doenças cardiovasculares (14, 51,85% e neuropatia diabética (11, 40,74%. Os fatores de risco mais prevalentes foram sedentarismo (39, 78,0%, obesidade central (7, 74,0% e HAS (35, 70,0%. Ainda são necessários grandes esforços na atenção primária ao paciente diabético com relação ao tratamento adequado da doença e à adesão de hábitos de vida saudáveis, pois a presença de complicações associadas ao estado de saúde geral dos pacientes diabéticos diminui significativamente a qualidade de vida desta população.

  10. SISTEM MONITORING LAYAR KOMPUTER SECARA REMOTE MENGGUNAKAN JARINGAN MULTIDROP RS 485

    Directory of Open Access Journals (Sweden)

    Irwan Kristanto

    1999-01-01

    Full Text Available Serial communication is commonly used in PC interface, but serial communication that is commonly used is RS-232 which only could communicate in one to one scheme. This paper will explain a software using the RS-485 interface that can communicate in one to many scheme using a multidrop network system. The server will monitor slave computer, so from the master computer we will be able to see what kind of application is being done in the slave computer. Software has been designed under Windows environtment (32 bit using Delphi 3.0 from Borland International, Inc Software. There are two kinds of software : master and slave. The master software will control the computer we want to monitor, while the slave software will send the monitor picture to the master. The system hardware uses 3 RS 232 to RS 485 converter installed at the 3 PCs, one converter is used by master, and 2 converters are used by slave computers. Testing of picture compression, picture transmission with varying transmission speed have been done in this experiment. Abstract in Bahasa Indonesia : Komunikasi serial banyak sekali digunakan dalam interface PC namun serial yang biasa dipakai adalah RS-232 yang hanya dapat berhubungan secara one to one. Dalam paper ini dibuat software menggunakan sistem RS-485 yang dapat berhubungan secara one to many menggunakan sistem multidrop network. Aplikasinya adalah monitoring komputer, jadi dalam sebuah komputer dapat melihat apa yang sedang dikerjakan oleh komputer lain dengan melihat layar monitor komputer tersebut. Software dibuat berbasis Windows (32 bit dengan bantuan Delphi 3.0 dari Borland International, Inc Software. Software terdiri dari dua yaitu master sebagai pengontrol untuk melihat komputer lain sedangkan slave sebagai pengirim gambar pada komputer yang dimonitor dan bersifat resident dan aktif. Hardware yang digunakan adalah 3 buah RS 232 to RS 485 Converter yang dipasang pada 3 buah PC dimana sebuah PC digunakan untuk master dan 2

  11. PEMBUATAN PERANGKAT LUNAK EDITOR DENAH RUANG 2D UNTUK DIVISUALISASIKAN SECARA 3D

    Directory of Open Access Journals (Sweden)

    Rudy Adipranata

    2006-01-01

    Full Text Available Usually we use paper to draw sketch of room for designing building. Using paper can cause a problem especially for changing the sketch or size of the room. To solve that problem, we can use software to design sketch of the room. Nowadays there are many software can be used but many of those can only view in two dimension, top side. Using only two dimensions viewing, user have difficulties to imagine the real room in three dimensions. Also there are software which can view in three dimensions, but unfortunately the price of those software is so high. In this paper, we implement software to help user to design the sketch of the room in two dimensions, but the result can be viewed in three dimensions. In three dimensions view, user can do movement like view the real world. This software also can be used to create building have multi level, and the accessories that can be used are floor, wall, door, window and stair. To implement this software we use OpenGL library. Abstract in Bahasa Indonesia : Untuk merancang denah ruang pada sebuah bangunan, biasanya dilakukan penggambaran sketsa ruang yang diinginkan pada kertas. Tidak semua orang, terutama orang yang awam terhadap bidang arsitektur, membayangkan sebuah gambar denah sebagai ruangan akan sangat menyulitkan. Untuk menjembatani antara perancang dengan pelanggan, antara rancangan denah dalam bentuk 2D dengan visualisasi dalam bentuk 3D, maka dalam penelitian ini dikembangkan sebuah perangkat lunak yang mampu menghasilkan visualisasi bentuk sebuah ruang dari inputan berupa gambar denah 2D tampak atas. Dengan adanya fasilitas editor pada perangkat lunak ini, maka perancang dapat melakukan perubahan denah ataupun ukuran yang sudah tergambar dan langsung menampilkan hasil visualisasinya. Oleh karena itu, pada penelitian ini, dikembangkan pembuatan perangkat lunak editor denah ruang secara 2D yang dapat langsung divisualisasikan dalam bentuk 3D dengan ukuran yang sama dengan yang diinputkan pada gambar

  12. VIRTUAL POINTER UNTUK IDENTIFIKASI ISYARAT TANGAN SEBAGAI PENGENDALI GERAKAN ROBOT SECARA REAL-TIME

    Directory of Open Access Journals (Sweden)

    M. Isa Irawan

    2008-01-01

    Full Text Available Many previous researches have been done in relation to human-robot interface, which is an interaction between human and robot using hand gesture. Hand gesture that is used in this research is a moving hand gesture with pointing position. The most important factor to identify hand gesture is the ability to differentiate hands with other objects based on the skin colour. A method to detect hand skin colour is using Fuzzy C-Means (FCM which can refine a cluster centre and the membership value of each data iteratively by minimizing objective function. Hence, the cluster centre moves to the correct location. Recognition result with moving detection method was able to detect the movement of a moving object 91.07944% in 1 second. Skin detection using FCM was able to segment skin colour and not the skin in real time with the successful rate 90.2834%. The successful rate of the hand gesture pattern identification using rule base is 86.67%. The successful rate of virtual hand writing using LVQ artificial neural network as a command for controlling a robot is 79.2%. Abstract in Bahasa Indonesia : Banyak penelitian sebelumnya berrhubungan dengan human robot interface, interaksi manusia dengan robot menggunakan isyarat tangan sebagai bahasa tubuh manusia. Isyarat tangan yang digunakan dalam penelitian ini adalah isyarat tangan bergerak yang berposisi menunjuk untuk identifikasi isyarat tangan, faktor yang paling penting adalah kemampuan membedakan tangan dengan obyek lain berdasarkan warna kulitnya. Metode untuk mendeteksi warna kulit tangan adalah Fuzzy C-Means (FCM yang memiliki kemampuan memperbaiki pusat cluster dan nilai keanggotaan tiap data secara berulang dengan meminimumkan fungsi obyektif, sehingga pusat cluster akan bergerak menuju lokasi yang tepat. Hasil pengenalan dengan metode moving detection, mampu mendeteksi pergerakan obyek bergerak. secara baik sebesar 91.07944% dalam 1 detik. obyek Skin detection dengan Fuzzy C-Means (FCM mampu melakukan

  13. Produksi Ramnolipid dan Polihidroksialkanoat secara Simultan dari Produk Samping Industri Biodiesel dengan Bahan Baku CPO

    Directory of Open Access Journals (Sweden)

    Darti Nurani

    2013-12-01

    Full Text Available Produksi bioplastik polyhydroxyalkanoates (PHA oleh bakteri menghadapi masalah biaya produksi yang tinggi. Masalah yang sama juga terjadi pada produksi ramnolipid biosurfaktan. Strategi baru telah diusulkan untuk mengurangi biaya produksi PHA, yaitu sel pertama diarahkan untuk menghasilkan produk ekstraseluler yaitu ramnolipid, lalu sel tersebut digunakan kembali untuk menghasilkan produk intrasleluer, yaitu PHA. Penelitian ini dilakukan untuk mempelajari produksi poli-hidroksialkanoat (PHA dan ramnolipid secara simultan dari sumber karbon yang relatif murah menggunakan teknik batch kultur. Dalam penelitian ini, produk samping industri biodiesel dari minyak sawit mentah (CPO digunakan sebagai sumber karbon dan sebagai pembanding digunakan minyak sawit. Hasil penelitian menunjukkan bahwa penggunaan sumber karbon produk samping CPO menghasilkan konsentrasi maksimum ramnolipid dan PHA masing-masing 80 mg/l dan 0,15 mg/l, yang dicapai pada konsentrasi sumber karbon 25 g/l dan waktu kultivasi 24 jam.

  14. Molar Dua Sulung Sebagai Salah Satu Pencetus Maloklusi Ditinjau Secara Radiografis (Laporan Kasus

    Directory of Open Access Journals (Sweden)

    Gimawati Muljono

    2015-10-01

    Full Text Available Pengetahuan mengenai kehilangan dini gigi molar-2 sulung sebagai penyebab miringnya gigi molar-1 tetap dan berkurangnya ruangan tempat erupsi gigi kaninus dan premolar, sudah lama diketahui. Dari penelusuran pustaka ternyata masih banyak faktor yang efeknya sama dan penyebabnya gigi molar-2 sulung yang kurang disadari dan belum mendapat perhatian sepenuhnya. Faktor-faktor tersebut adalah : anatomis, karies, ankilose, dan iatrogenik, yang efek lanjutnya bahkan dapat sampai menimbulkan maloklusi. Makalah ini akan mengemukakan suatu kasus, sehubungan dengan keadaan gigi molar-2 sulung yang dapat menjadi salah satu faktor penyebab maloklusi ditinjau secara radiografis dengan foto panoramik dan cephalometri. Ramalan maloklusi gigi geligi tetap dapat dilihat dari proyeksi gambar pergeseran gigi-gigi, kontak oklusi bidang terminal permukaan distal mahkota gigi molar-2 sulung, hubungan kontak gigi molar-1 tetap di foto pasien.

  15. PENGEMBANGAN APLIKASI MANAJEMEN PROYEK SECARA GRAFIS BERBASIS WEB MENGGUNAKAN METODE DIAGRAM PRESEDENSI

    Directory of Open Access Journals (Sweden)

    Suhadi Lili

    2004-07-01

    Full Text Available Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Dalam kegiatan manajemen proyek diperlukan koordinasi yang bagus dan kerjasama tim yang kompak antar divisi pelaksana proyek. Selama ini, secara kovensional untuk membuat perencanaan proyek maupun melakukan review dan evaluasi jalannya proyek, perlu diadakan rapat atau pertemuan langsung dari orang-orang yang berperan sebagai manajer dan perencana. Para manajer profesional ini seringkali adalah orang-orang yang mobilitasnya tinggi sehingga tidak selalu berada ditempat pada saat dibutuhkan, sehingga koordinasi proyek akan terhambat dan menjadi tidak efisien. Dengan adanya kemajuan teknologi pada saat ini khususnya internet, permasalahan tersebut sangat memungkinkan untuk diselesaikan dengan menyediakan perangkat lunak yang dikhususkan untuk manajemen proyek, yang bisa diakses online secara multiuser dengan pendekatan sifat real time sehingga para manajer dapat melakukan kolaborasi kerja di mana saja mereka berada selama tersedia perangkat komputer yang terhubung dengan internet. Penelitian ini bertujuan untuk membuat perangkat lunak tersebut. Dalam Teknik Informatika, aplikasi seperti ini merupakan aplikasi terdistribusi, karena itu idealnya harus dibuat bersifat portabel agar dapat berjalan pada semua sistim

  16. Pembuatan Biodiesel Dari Minyak Kelapa Dengan Katalis NaOH Menggunakan Gelombang Mikro (Microwave Secara Kontinyu

    Directory of Open Access Journals (Sweden)

    Daru Satria Prayanto

    2016-04-01

    Full Text Available Biodiesel merupakan bioenergi atau bahan bakar nabati yang dibuat dari minyak nabati melalui proses transesterifikasi, esterifikasi, atau proses esterifikasi-transesterifikasi. Proses pembuatan biodiesel dapat dilakukan dengan metode pemanasan konvensional maupun dengan metode pemanasan microwave. Dengan radiasi microwave, maka waktu yang dibutuhkan saat proses transesterifikasi lebih singkat dibandingkan dengan konvensional. Disisi lain, minyak kelapa memiliki potensi yang besar untuk digunakan sebagai bahan baku dalam pembuatan biodiesel karena ketersediaannya yang melimpah. Penelitian ini bertujuan untuk membuat biodiesel dari minyak kelapa secara kontinyu melalui proses transesterifikasi metanol dengan menggunakan radiasi microwave dengan katalis NaOH dan mempelajari pengaruh konsentrasi tiap katalis, daya, dan laju umpan yang digunakan terhadap yield, densitas, dan viskositas biodiesel yang dihasilkan. Dalam penelitian ini di gunakan 3 variabel, yaitu laju umpan 0,73; 1,25; 1,72 ml/s, konsentrasi katalis 0,25; 0,5; 1 (% berat variabel daya microwave 100, 264, 400, 600, dan 800 Watt. Rasio umpan ditentukan pada 1:9. Pada tahap persiapan melarutkan metanol dan katalis sesuai dengan variabel hingga tercampur homogen. Selanjutnya tahap transesterifikasi dengan mencampurkan larutan metanol (metanol dan katalis dengan minyak kelapa dengan mol ratio yang telah ditentukan dan mengatur daya microwave untuk memulai proses transesterifikasi, proses berlangsung secara kontinyu menggunakan mix flow reaktor. Selanjutnya pemisahan hasil transesterifikasi dari gliserol, dilanjutkan dengan tahap pencucian dengan aquadest untuk memisahkan impurities dan katalis yang masih tersisa dalam biodiesel kemudian memanaskan pada oven untuk menguapkan kandungan air dalam biodiesel. Selajutnya menganalisisa hasil biodiesel terhadap yield, densitas, dan viskositasnya. Hasil terbaik dari variabel yang digunakan di atas adalah pada katalis NaOH dengan konsentrasi 1

  17. LEP、APN与多囊卵巢综合征的相关性研究%Study on the Correlation Between LEP, APN and Polycystic Ovary Syndrome

    Institute of Scientific and Technical Information of China (English)

    陈育娟

    2014-01-01

    目的探讨血清瘦素(LEP)和脂联素(APN)与多囊卵巢综合征(PCOS)之间的关系。方法选择2012年8月~2013年8月确诊PCOS的女性39例作为PCOS组。因单纯输卵管因素或/及男方因素不孕就诊的女性42例为非PCOS组。两组再分肥胖组和非肥胖组。检测早卵泡期FSH、LH、T、FPG、FINS、计算HOMA-IR。另测定血清LEP、APN,计算L/A。结果①PCOS组LEP水平及L/A比值显著高于非PCOS组,两组肥胖者均较非肥胖者升高。②PCOS组APN水平显著低于非PCOS组,PCOS组肥胖者较非肥胖者下降。③相关性分析:所有研究对象LEP及L/A比值与HOMA-IR成正相关,APN则与之成负相关。④多元回归分析:单独的LEP或APN指标与PCOS的相关性均不强烈,而L/A比值则与患病有明显相关。结论 LEP、APN可能参与PCOS的发生、发展过程,L/A比值是优于单独的APN或LEP的一个新指标,是PCOS患病的独立危险因素。%Objective To investigate the development of PCOS and the influent factors among the LEP,APN.Methods 39 PCOS patients were sampled in experimental group,and 42 patients with pure tubal factor and/or male factor infertility were randomly assigned as control group.Then the two groups were divided into obese and non-obese group.The fol owing outcomes were measured in four groups: ①early fol icular phase serum FSH、LH、T、FPG、FINS;and count HOMA-IR;(2)the serum LEP,APN,and count L/A ratio.Results ①the levels of LEP and the L/A ratios in PCOS patients were higher than that of the non-PCOS patients,the levels of LEP in obese patients were higher than that of non-obese patients. ②the levels of APN in PCOS patients were lower than that of the non-PCOS patients.The levels of APN in obese patients were lower than that of non-obese patients in PCOS patients. ③Cor elation analysis:The level of LEP and L/A ratio was positively correlated with HOMA-IR. ④Multivariate regression analysis:there was no significant cor

  18. Experience of Quality Care Ward to Implement APN Scheduling%在优质护理病房实行APN排班的体会

    Institute of Scientific and Technical Information of China (English)

    刘颖

    2014-01-01

    目的探讨在优质护理病房实行APN排班模式的可行性。方法改革传统排班,根据学历、职称、工作能力的不同,将科室护理人员合理搭配后实施APN排班模式,为患者提供连续的整体服务。结果APN排班模式减少了交接班次数,实现了护士双班制值班,保障了护理安全,做到让患者满意、护士满意、医生满意。结论APN排班模式是可行的,使护理人力资源使用更加合理,而且实现了科室护士的分层级管理,有助于优质护理服务工作的推进。%Objective To investigate the feasibility of the implementation of quality care ward APN scheduling model. Methods Reform the traditional scheduling methods, depending on education, job title, ability to work, the department wil implement APN scheduling nurses with a reasonable model for the patient to provide continuous service as a whole. Results APN scheduling mode reduces shift times to achieve a double-shift nurse on duty to ensure the safety of care, so that patient satisfaction, nurse satisfaction, physician satisfaction. Conclusion APN scheduling model is feasible to make nursing a more rational use of human resources, but also to achieve a hierarchical management department nurses to help promote quality care work.

  19. Penetapan Kadar Parasetamol, Kafein Dan Asetosal Dalam Sediaan Oral Secara Simultan Dengan Metode Kromatografi Cair Kinerja Tinggi (KCKT)

    OpenAIRE

    Pane, Rika Khairyah

    2011-01-01

    Obat dalam betuk kombinasi sering digunakan untuk mengobati berbagai penyakit, termasuk diantaranya penyakit Flu. Kombinasi dari Parasetamol, Kafein dan Asetosal biasanya digunakan untuk memperkuat efek analgetiknya. Obat ini dapat dianalisis secara serempak (simultan) mengunakan metode Kromatografi Cair Kenerja Tinggi (KCKT). Dalam beberapa literatur menyatakan untuk penetapan kadar campuran dari Parasetamol, Kafein dan Asetosal dapat dilakukan dengan beberapa komposisi fase gerak antar...

  20. Silencing of CYP6 and APN genes affects the growth and development of rice yellow stem borer, Scirpophaga incertulas

    Directory of Open Access Journals (Sweden)

    Vijaya Sudhakara Rao eKola

    2016-02-01

    Full Text Available RNAi is a powerful tool to target the insect genes involved in host-pest interactions. Key insect genes are the choice for silencing to achieve pest derived resistance where resistance genes are not available in gene pool of host plant. In this study, an attempt was made to determine the effect of dsRNA designed from two genes Cytochrome P450 derivative (CYP6 and Aminopeptidase N (APN of rice yellow stem borer (YSB on growth and development of insect. The bioassays involved injection of chemically synthesized 5ꞌ FAM labeled 21-nt dsRNA into rice cut stems and allowing the larvae to feed on these stems which resulted in increased mortality and observed growth and development changes in larval length and weight compared with its untreated control at 12-15 days after treatment. These results were further supported by observing the reduction in transcripts expression of these genes in treated larvae. Fluorescence detection in treated larvae also proved that dsRNA was readily taken by larvae when fed on dsRNA treated stems. These results from the present study clearly show that YSB larvae fed on dsRNA designed from Cytochrome P450 and Aminopeptidase N has detrimental effect on larval growth and development. These genes can be deployed to develop YSB resistance in rice using RNAi approach.

  1. Silencing of CYP6 and APN Genes Affects the Growth and Development of Rice Yellow Stem Borer, Scirpophaga incertulas.

    Science.gov (United States)

    Kola, Vijaya Sudhakara Rao; Renuka, P; Padmakumari, Ayyagari Phani; Mangrauthia, Satendra K; Balachandran, Sena M; Ravindra Babu, V; Madhav, Maganti S

    2016-01-01

    RNAi is a powerful tool to target the insect genes involved in host-pest interactions. Key insect genes are the choice for silencing to achieve pest derived resistance where resistance genes are not available in gene pool of host plant. In this study, an attempt was made to determine the effect of dsRNA designed from two genes Cytochrome P450 derivative (CYP6) and Aminopeptidase N (APN) of rice yellow stem borer (YSB) on growth and development of insect. The bioassays involved injection of chemically synthesized 5' FAM labeled 21-nt dsRNA into rice cut stems and allowing the larvae to feed on these stems which resulted in increased mortality and observed growth and development changes in larval length and weight compared with its untreated control at 12-15 days after treatment. These results were further supported by observing the reduction in transcripts expression of these genes in treated larvae. Fluorescence detection in treated larvae also proved that dsRNA was readily taken by larvae when fed on dsRNA treated stems. These results from the present study clearly show that YSB larvae fed on dsRNA designed from Cytochrome P450 and Aminopeptidase N has detrimental effect on larval growth and development. These genes can be deployed to develop YSB resistance in rice using RNAi approach.

  2. CD 13/APN expression in peripheral blood lymphocytes and skin lesions in patients with advanced psoriasis vulgaris

    Institute of Scientific and Technical Information of China (English)

    Liu Taihua; Liu Defang; Chen Yihua; Hu Zonghai; Chen Lu; Luo Chen; Xu Zhejuan

    2009-01-01

    Objective: To observe the expression of CDI3/APN in peripheral blood lymphocytes and skin lesions of patients with advanced psoriasis vulgaris, and discuss its effect on the pathogenesis of psoriasis. Methods: CDI 3 expression in peripheral blood lymphocytes and skin lesions was detected by flow cytometry and immunohistochemical technique, respectively. Results were compared with those of healthy controls. Results: CD13 expression was significantly higher in peripheral blood lymphocytes of patients with advanced psoriasis vulgaris than in that of healthy controls, and in skin lesions than in healthy skin tissues. The expression was mainly in the suprabasal layers of skin lesions, andpositively correlated to PASI (R=0.78029). Conclusion: The significantly higher expression of CDI3 in peripheralblood lymphocytes and skin lesions of the patients with advanced psoriasis vulgaris probably is related to immunological abnormality, blood vessel abnormality and proliferation of keratinocyte in the pathogenic course of psoriasis. It may be a novel and effective way to treat psoriasis with specific CD13 inhibitors.

  3. Efek Antibakteri Ekstrak Daun Mimba (Azadirachta indica A. Juss terhadap Bakteri Vibrio algynoliticus Secara In Vitro

    Directory of Open Access Journals (Sweden)

    Uli Ayini

    2014-03-01

    Full Text Available Budidaya udang windu di Indonesia telah berkembang pesat. Salah satu kendala budidaya udang adalah penyakit Vibriosis yang disebabkan oleh bakteri Vibrio algynoliticus. Tujuan penelitian ini adalah untuk mengetahui efek antibakeri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus. Penelitian ini menggunakan metode dilusi untuk mengetahui efek antibakteri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus secara in vitro. Konsentrasi ekstrak yang digunakan (% yaitu: 0; 2,5; 5; 7,5; 10; 12,5 dan sebagai kontrol terdiri dari kontrol positif, dan kontrol negatif. Pengumpulan data untuk menentukan MIC (Minimum Inhibitory Concentration dilakukan dengan membandingkan kejernihan kultur di medium TSB 2% pada berbagai konsentrasi yang berbeda, dengan kontrol positif dan kontrol negatif. Penentuan MBC (Minimum Bacterisidal Concentration dilakukan dengan melihat ada tidaknya dan jumlah koloni bakteri Vibrio alginolyticus yang muncul pada medium subkultur TSA 2% setelah inkubasi 24 jam. Hasil penelitian menunjukkan nilai MIC yaitu konsentrasi 5%, hal ini ditunjukkan dengan tabung yang mulai jernih. Nilai MBC ekstrak daun mimba terhadap bakteri Vibrio alginolyticus adalah konsentrasi 12,5% ditandai dengan sudah tidak munculnya  koloni bakteri Vibrio alginolyticus. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak daun mimba dapat memberikan efek antibakteri terhadap bakteri Vibrio alginolyticus secara in vitro.Tiger shrimp cultivation in Indonesia has been growing rapidly. The main obstacle is the shrimp farming vibriosis disease caused by the bacterium Vibrio algynoliticus. The aim of this research was to determine the effects of neem leaf extract antibakeri against Vibrio algynoliticus. This study used a dilution method to determine the antibacterial effect of neem leaf extract against Vibrio algynoliticus bacteria in vitro. The concentration of the extract used (%: 0; 2.5; 5; 7.5; 10; 12.5 and as a control consisting of a positive

  4. Efek Antibakteri Ekstrak Daun Mimba (Azadirachta indica A. Juss terhadap Bakteri Vibrio algynoliticus Secara In Vitro

    Directory of Open Access Journals (Sweden)

    Uli Ayini

    2014-03-01

    Full Text Available Budidaya udang windu di Indonesia telah berkembang pesat. Salah satu kendala budidaya udang adalah penyakit Vibriosis yang disebabkan oleh bakteri Vibrio algynoliticus. Tujuan penelitian ini adalah untuk mengetahui efek antibakeri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus. Penelitian ini menggunakan metode dilusi untuk mengetahui efek antibakteri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus secara in vitro. Konsentrasi ekstrak yang digunakan (% yaitu: 0; 2,5; 5; 7,5; 10; 12,5 dan sebagai kontrol terdiri dari kontrol positif, dan kontrol negatif. Pengumpulan data untuk menentukan MIC (Minimum Inhibitory Concentration dilakukan dengan membandingkan kejernihan kultur di medium TSB 2% pada berbagai konsentrasi yang berbeda, dengan kontrol positif dan kontrol negatif. Penentuan MBC (Minimum Bacterisidal Concentration dilakukan dengan melihat ada tidaknya dan jumlah koloni bakteri Vibrio alginolyticus yang muncul pada medium subkultur TSA 2% setelah inkubasi 24 jam. Hasil penelitian menunjukkan nilai MIC yaitu konsentrasi 5%, hal ini ditunjukkan dengan tabung yang mulai jernih. Nilai MBC ekstrak daun mimba terhadap bakteri Vibrio alginolyticus adalah konsentrasi 12,5% ditandai dengan sudah tidak munculnya  koloni bakteri Vibrio alginolyticus. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak daun mimba dapat memberikan efek antibakteri terhadap bakteri Vibrio alginolyticus secara in vitro.Tiger shrimp cultivation in Indonesia has been growing rapidly. The main obstacle is the shrimp farming vibriosis disease caused by the bacterium Vibrio algynoliticus. The aim of this research was to determine the effects of neem leaf extract antibakeri against Vibrio algynoliticus. This study used a dilution method to determine the antibacterial effect of neem leaf extract against Vibrio algynoliticus bacteria in vitro. The concentration of the extract used (%: 0; 2.5; 5; 7.5; 10; 12.5 and as a control consisting of a positive

  5. Uji Efektivitas Beberapa Minyak Atsiri terhadap Pertumbuhan Microsporum canis secara in Vitro

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    Bunga Saridewi Nurmansyah

    2016-01-01

    Full Text Available AbstrakDermatofitosis merupakan masalah kesehatan masyarakat yang penting di daerah tropis. Minyak atsiri merupakan salah satu potensi alam Indonesia yang diketahui memiliki daya antifungi. Tujuan penelitian ini adalah mengetahui efektivitas beberapa minyak atsiri (serai wangi, kayu manis dan cengkeh sebagai antijamur dalam mengendalikan pertumbuhan Microsporum canis penyebab dermatofitosis secara in vitro. Penelitian dilakukan di Laboratorium Fitopatologi KP Balittro Laing Solok dari  Februari sampai April 2014. Studi eksperimental ini dilakukan dengan metode pengenceran disusun  dalam Desain Rancang Acak Lengkap dalam Faktorial. Faktor pertama adalah jenis minyak atsiri (daun serai wangi, daun kayu manis, daun cengkeh. Faktor kedua adalah tingkat konsentrasi minyak atsiri (100 ppm, 250 ppm, 500 ppm, 1000 ppm dan 2000 ppm. Hasil penelitian ini menunjukkan bahwa minyak atsiri daun serai wangi, daun kayu manis dan daun sarasah cengkeh efektif dalam menekan pertumbuhan M. canis secara in vitro. Ketiga minyak atsiri pada konsentrasi 500 ppm telah mampu menghambat pertumbuhan M. canis hingga 100%. Minyak atsiri daun sarasah cengkeh memiliki efek antifungi paling tinggi (89,17%, diikuti minyak atsiri daun serai wangi (80,98% dan kayu manis (77,07%.                Kata kunci: minyak atsiri, serai wangi, cengkeh, kayumanis, microsporum canis AbstractDermatophytosis is an important public health problem in tropical areas. Essential oil is one of natural potential from Indonesia has been predicted as antifungal. The objective of this study was to detect effectivity some essential oils such as citronella, cinnamon and clove as antifungal to control the growth of dermatophyte infections caused by Microsporum canis by in vitro . The study was done  in the Laboratory of Phytopathology KP Balitro of Laing Solok from February until April 2014. This is an experimental study with dilution method arranged in Complete Randomized Design in

  6. 棉铃虫中肠氨肽酶 APN4与 Cry1Ac、Cry2Aa 结合能力的比较%Comparison of the binding affinity of the APN4 receptor in Helicoverpa armigera (Hübner) to the Cry1Ac and Cry2Aa insecticidal proteins

    Institute of Scientific and Technical Information of China (English)

    袁向东; 葛朝虹; 肖玉涛; 梁革梅

    2015-01-01

    Objectives] Bt (Bacillus thuringiensis) insecticidal proteins have been widely adopted to control agricultural pests because of their high target specificity. The binding of a Bt insecticidal protein to its specific receptor in the insect midgut plays a key role in the insecticidal action process. Aminopeptidase N (APN) is one of the major Bt protein receptors. To better characterize the molecular mechanism underlying the insecticidal activity of different Bt insecitcidal proteins, and lay the foundation for Bt resistance management and novel Bt insecticidal protein development, we analyzed the binding affinity of aminopeptidase N4 (APN4) to the Cry1Ac and Cry2Aa insecitcidal proteins in Helicoverpa armigera. [Methods] The binding affinity of aminopeptidase N4 (APN4) was assessed using ligand blot analysis and an ELISA binding assay, respectively. [Results] The results show that recombinant APN4 could bind to both Cry1Ac and Cry2A; their respective dissociation constants were 46.7 nmol/L and 26.5 nmol/L. [Conclusion] The results suggest that there was no significant difference in the binding affinity of APN4 to Cry1Ac and Cry2Aa in H. armigera.%【目的】 Bt 杀虫蛋白(Bacillus thuringiensis)具有高度的靶标特异性,已经被广泛用于农业害虫防治。Bt 杀虫蛋白要发挥杀虫活性,必须首先与其受体蛋白结合,氨肽酶 N(Aminopeptidase N)是一类重要的 Bt 受体蛋白。因此,分析该受体与 Bt 杀虫蛋白的结合能力,可为进一步明确不同 Bt 的分子作用机制、Bt 的抗性治理以及新 Bt 的开发应用等提供借鉴。【方法】本文利用 Ligand blot 和 Elisa 方法比较了棉铃虫 Helicoverpa armigera 中肠 APN4(Aminopeptidase N4,APN4)与 Cry1Ac、Cry2Aa 的结合能力。【结果】原核表达的 APN4片段与活化的 Cry1Ac、Cry2Aa 都可以结合,解离常数(Kd)分别是48.59 nmol/L和21.73 nmol/L。【结论】 APN4片段与 Cry1Ac、Cry2Aa 的结合能力

  7. Iodidimonas muriae gen. nov., sp. nov., an aerobic iodide-oxidizing bacterium isolated from brine of a natural gas and iodine recovery facility, and proposals of Iodidimonadaceae fam. nov., Iodidimonadales ord. nov., Emcibacteraceae fam. nov. and Emcibacterales ord. nov.

    Science.gov (United States)

    Iino, Takao; Ohkuma, Moriya; Kamagata, Yoichi; Amachi, Seigo

    2016-12-01

    A chemo-organotrophic iodide (I-)-oxidizing bacterial strain, C-3T, isolated from natural gas brine of an iodine recovery facility in Kujukuri, Chiba, Japan, was characterized for representation of a novel species in the class Alphaproteobacteria. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbours of strain C-3T were members of the genera Eilatimonas, Kordiimonas, Rhodothalassium and Temperatibacter with 88-91 % sequence similarity. Cells of strain C-3T were aerobic, Gram-staining-negative, non-sporulating and rod-shaped (1.3-3.6 µm in length). Strain C-3T grew optimally at 30 °C, pH 7.5 and with 3 % NaCl (w/v). Iodide oxidation to form molecular iodine (I2) was a unique trait for strain C-3T, whereas the strain did not utilize iodide as a sole electron donor for chemolithoautotrophic growth. The major isoprenoid quinone was Q-10. The major cellular fatty acids were C18 : 1ω7c and C16 : 1ω5c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The G+C content of the genomic DNA was 58.5 mol%. Iodide oxidation and the major cellular fatty acids composition distinguished strain C-3T from phylogenetically related bacteria. On the basis of the phenotypic features and the phylogenetic position, a novel genus and species are proposed for strain C-3T (=JCM 17843T=LMG 28660T), to be named Iodidimonas muriae gen. nov., sp. nov. We also propose to place the distinct sublineages of the genera Iodidimonasgen. nov. and Emcibacter in the orders Iodidimonadales ord. nov. and Emcibacterales ord. nov., respectively, because these genera are located far apart from the order Kordiimonadales and form the distinct lineage in the class Alphaproteobacteria.

  8. Dinamika Leukosit Akibat Xilazin pada Anjing Lokal yang Dianestesi Ketamin secara Subkutan

    Directory of Open Access Journals (Sweden)

    Komang Sri Adiari

    2016-06-01

    Full Text Available Dinamika leukosit dapat diketahui dari pemberian xilazin dengan berbagai dosis pada anjing yang dianestesi ketamin secara subkutan. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL Split In Time dengan empat perlakuan yaitu X2K10 sebagai kontrol (xilazin dosis 2 mg/kg IM, ketamin dosis 10 mg/kg IM, X4K10 (xilazin dosis 4 mg/kg SC, ketamin dosis 10 mg/kg SC, X6K10 (xilazin dosis 6 mg/kg SC, ketamin dosis 10 mg/kg SC, dan X8K10 (xilazin dosis 8 mg/kg SC, ketamin dosis 10 mg/kg SC. Tiap perlakuan menggunakan 6 ekor anjing lokal sehingga jumlah yang digunakan sebanyak 24 ekor. Pengambilan sampel darah dilakukan selama anestesi dengan selang waktu 20 menit sampai menit ke-100 untuk pemeriksaan total leukosit dan diferensial leukosit. Hasil penelitian menunjukan bahwa terjadi peningkatan rata-rata total leukosit dan fluktuasi diferensial leukosit, namun peningkatannya berada pada rentang normal anjing lokal. Disimpulkan bahwa dosis xilazin tidak memberikan efek yang nyata (P>0,05 terhadap total dan diferensial leukosit, namun waktu pemeriksaan sampel darah menunjukkan efek sangat nyata (P0,05 terhadap basofil dan monosit.

  9. PERTUMBUHAN SPORA RUMPUT LAUT Gracilaria verrucosa SECARA IN VITRO DENGAN PENAMBAHAN HORMON PENGATUR PERTUMBUHAN PADA TANAMAN

    Directory of Open Access Journals (Sweden)

    Emma Suryati

    2016-11-01

    Full Text Available Perbaikan mutu genetik rumput laut Gracilaria verrucosa dapat dilakukan melalui perkawinan silang dari spora atau melalui fusi protoplas. Perkembangan spora rumput laut sangat tergantung pada media tumbuh dan kondisi lingkungan dimana spora tersebut berada. Untuk memacu perkembangan spora pada kondisi laboratorium, diperlukan perlakuan khusus agar spora dapat tumbuh dan bertahan hidup. Pemberian kinetin yang berfungsi sebagai hormon perangsang tumbuh pada tanaman dapat mempercepat pertumbuhan dari spora membentuk anakan baru. Konsentrasi kinetin yang digunakan berkisar antara 0,5–2 mg/L. Sintasan spora dan pertumbuhan yang optimum diperoleh pada pemberian kinetin 1 mg/L baik pada media padat maupun pada media cair. Pemeliharaan anakan rumput laut secara in vitro dipertahankan hingga mencapai ukuran 2 cm. Genetic improvement of seaweed, G. verrucosa can be done through spore cross breeding or protoplast fusion. Seaweed spore development is very dependent on the medium and environment where the spore grows. To stimulate the development of the spore in laboratory, special treatment is needed in order to foster spore growth and survival. Kinetin treatment as a plant growth regulator can stimulate the growth of seaweed from spore to bud. Applied kinetin range was 0.5–2 mg/L. Optimum survival rate and growth of spore achieved by the addition of 1 ppm kinetin concentration both in solid and liquid media. In vitro nursery of buds was continued until the buds reached 2–3 cm in size.

  10. Kapasitas Adsorpsi Bentonit terhadap Sulfur dan Merkuri secara Simultan pada Pembakaran Batubara

    Directory of Open Access Journals (Sweden)

    Alhamidi Yusran

    2015-12-01

    Full Text Available Pemanfaatan batubara sebagai sumber energi melalui pembakaran langsung akan menghasilkan emisi gas, partikulat trace metal (logam berat dan abu (terutama abu terbang yang akan mencemari udara. Penanganan terhadap pencemaran tersebut merupakan hal yang sangat mendesak. Dalam studi ini ditawarkan penyelesaian secara simultan terhadap emisi SOx dan partikulat logam berat merkuri pada pembakaran batubara peringkat rendah yang ada di Aceh melalui penyerapan menggunakan bentonit alam yang juga terdapat di Aceh (juga di daerah lain di Indonesia. Penggunaan bentonit dapat mengurangi emisi gas SO2 dan partikulat trace metal Hg dalam gas buang dan abu terbang. Bentonit dapat meningkatkan afinitas atau gaya tarik menarik antara Hg dan mineral-mineral dalam bentonit dan sekaligus menurunkan afinitas Hg terhadap S atau SO2. Konsentrasi bentonit dalam kajian ini, tanpa kalsinasi dan langsung dicampur dalam batubara, adalah 0 – 16% dan temperatur pembakaran adalah 700 – 900oC. Hasil yang didapat menunjukkan bahwa kondisi maksimum penyerapan sulfur dan/atau SO2 serta merkuri terjadi pada kandungan bentonit 6% dan temperatur 700oC.

  11. Sistem Pengendali Peralatan Elektronik dalam Rumah secara Otomatis Menggunakan Sensor PIR, Sensor LM35, dan Sensor LDR

    Directory of Open Access Journals (Sweden)

    Eka Desyantoro

    2015-08-01

    Full Text Available Listrik merupakan hal yang sangat penting di kehidupan kita. Setiap pekerjaan kita pasti dibantu dengan adanya listrik. Mulai dari penerangan hingga pengaturan suhu ruangan pun semuanya dibantu oleh listrik. Ketergantungan manusia terhadap listrik ini menimbulkan kebiasaan buruk. Banyak orang yang terkadang membiarkan suatu peralatan elektronik hidup pada saat tidak dibutuhkan. Terjadi suatu permasalahan untuk menciptakan suatu desain sistem embedded untuk mengendalikan peralatan elektronik dalam rumah secara otomatis. Makalah ini membahas tentang perancangan sistem pengendali peralatan elektronik dalam rumah secara otomatis. Sistem terdiri dari sensor PIR yang berfungsi untuk mendeteksi objek bergerak (manusia, sensor LM35 yang berfungsi untuk mendeteksi suhu, dan sensor LDR berfungsi sebagai sensor cahaya. Mikrokontroller ATMega16 sebagai pengendali jalannya sistem dari pembacaan sensor, menampilkan data sensor pada LCD dan mengatur kontak relay untuk menghidup dan mematikan listrik. Sistem pengendali peralatan elektronik dalam rumah secara otomatis menunjukan sensor LDR dapat membedakan gelap dan terang, sensor suhu LM35 dapat mendeteksi suhu dalam ruangan dengan toleransi kesalahan pembacaan kurang lebih 2o Celcius, dan sensor PIR dapat mendeteksi pergerakan manusia sejauh 5 meter.

  12. Relação entre hipertensão arterial sistêmica e síndrome da apnéia obstrutiva do sono

    Directory of Open Access Journals (Sweden)

    Cavallari Fransérgio E.M.

    2002-01-01

    Full Text Available Introdução: hipertensão arterial sistêmica (HAS e a Síndrome da Apnéia Obstrutiva do Sono (SAOS são doenças prevalentes na população mundial e, embora despertem interesse na comunidade médica, não têm fisiopatologia e mecanismos pelo qual produzem suas complicações completamente elucidados. A SAOS está cada vez mais relacionada a patologias cardiovasculares, principalmente à hipertensão, que é prevalente em pacientes com SAOS. Objetivo: Em nosso estudo, objetivamos avaliar as alterações encontradas na presença de HAS em pacientes portadores de SAOS. Forma de estudo: Clínico prospectivo randomizado. Material e método: Estudamos uma população de pacientes do Ambulatório de Ronco e Apnéia do HC FMRP - USP, de fevereiro de 1999 a fevereiro de 2002, em um total de 42, sendo 30 (71,42% pacientes homens e 12 (28,58% mulheres, com idades de 21 a 77 anos (média de 45,3, dp = 9,372706, todos com diagnóstico de SAOS confirmado pela polissonografia, divididos em dois grupos de 21 pacientes com SAOS (controle e 21 com SAOS e HAS. Avaliamos as escalas de Epworth, Ronco e Sonolência nos dois grupos, além do uso da polissonografia para estudo objetivo. Resultado: Não encontramos diferença estatística entre os grupos na comparação dos resultados das escalas, mesmo tendo o grupo de hipertensos médias maiores. Ao avaliarmos objetivamente os pacientes com a polissonografia, levando em conta o IDR, há estatística significante (p=0,0326, com os hipertensos apresentando médias maiores. Vendo a proporção nos grupos, há predomínio de pacientes com apnéia moderada e grave entre os hipertensos, e leve entre os do controle. Conclusão: Concluindo, a concomitância entre HAS e SAOS representa fator agravante no grau da apnéia.

  13. 外科实施APN连续性排班方式的效果与体会%The effect and experience of APN continuity scheduling method

    Institute of Scientific and Technical Information of China (English)

    黄房珍

    2012-01-01

    Objective:To study the effect and nursing management experience of surgical nurse implementation APN continuity scheduling method . Meth-ods:A group's work time from 8:00 -12:00,14:30 -17:30 adjusted to 8:00 -16:00. P group's work time from 17:30 -1:00 adjusted to 16:00 -22: 00. N group's work time adjusted to 22:00 - 8:00 . Each shift team has a leader. Implementation of APN continuity scheduling method not only give full play to the advantage of nursing leader management experience, but also make good use of good physical quality of young nurse . Results: APN continuity scheduling method can reduce repeated handing - over time, frequency, improve work efficiency, and guarantee the quality of work and nursing safety. Conclusion:After implementation APN continuity scheduling method, nursing human resource can get rational application, develop nursing staff potential maximumly and improve working efficiency.%目的:探讨外科护士实施APN连续性排班方式的效果.方法:将A班上班时间由8:00~12:00,14:30~17:30调整为:8:00~16:00;P班由17:30~1:00调整为16:00~22:00;N班由1:00~8:00调整为22:00~8:00.实施APN连续性排班方式.结果:APN连续性排班方式能够减少重复交接班时间、次数,提高工作效率,有效保证了病区护理工作质量和护理安全.结论:外科护士实施APN连续性排班方式后,护理人力资源能够得到合理使用,最大限度的发挥护理人员的潜能,确实提高了工作效率.

  14. KERAGAAN BENIH IKAN PATIN SIAM (Pangasianodon hypophthalmus YANG DITEBAR SECARA LANGSUNG DI KOLAM PADA UMUR BERBEDA

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    Didik Ariyanto

    2012-08-01

    keberhasilan pemijahan buatan pada tahun 1981. Pada pelaksanaannya, pembenihan ikan patin siam dilakukan secara indoor hatchery. Dalam sistem tersebut, dibutuhkan nauplii Artemia sebagai pakan awal larva ikan patin siam yang dilanjutkan dengan pemberian Moina dan Daphnia beku serta cacing darah (Tubifex sebelum pemberian pakan buatan. Hal ini mengakibatkan adanya ketergantungan usaha pembenihan ikan patin siam terhadap pasokan beberapa jenis pakan alami tersebut. Penelitian ini bertujuan untuk mengevaluasi keragaan ikan patin siam yang ditebar di kolam pendederan pada umur yang berbeda. Sasaran yang akan dicapai adalah menghilangkan ketergantungan pembenihan ikan patin siam terhadap pasokan beberapa jenis pakan larva ikan patin siam dan disubstitusi dengan pakan alami yang ada di kolam. Pada percobaan ini, larva patin siam ditebar pada umur 5, 10, dan 15 hari setelah menetas. Larva ditebar di kolam pendederan seluas 200 m2 dengan kepadatan 100 ekor/m2. Pemeliharaan dilakukan selama 2 bulan. Hasil penelitian menunjukkan bahwa benih ikan patin siam yang ditebar di kolam pada umur 5 hari setelah menetas mempunyai laju pertumbuhan spesifik sebesar 11,12% lebih baik daripada benih ikan patin siam yang ditebar pada umur 10 dan 15 hari setelah menetas, yaitu sebesar 8,41% dan 8,65%. Namun demikian, bobot individu (9,81-12,32 g, biomassa panen (118,77-141,91 kg serta sintasan (54,53%-71,49% pada akhir percobaan tidak berbeda nyata.

  15. Pemanfaatan Biji Turi Sebagai Bahan Baku Pembuatan Kecap Secara Hidrolisis dengan Menggunakan Ekstrak dan Nanas

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    Aminah Asngad

    2015-04-01

    Full Text Available Selama ini pemanfaatan tanaman turi oleh  masyarakat masih terbatas, bagian dari tanaman turi yang banyak dimanfaatkan oleh masyarakat hanya bunganya.  Padahal biji turi yang berbentuk bulat berwarna kuning kecoklatan mempunyai rasa dan aroma khas jenis kacang-kacangan juga dapat dimanfaatkan karena kaya dengan mineral dan vitamin serta mengandung protein. Biji dari tanaman turi dapat digunakan sebagai bahan baku alternatif  dalam pembuatan  kecap karena biji tanaman turi tersebut  mempunyai komposisi  kandungan gizi yang tidak jauh berbeda dengan kedelai, terutama kandungan  protein biji turi sebesar 36,21%  yang setara  dengan kandungan protein kedelai sebesar 37,5% Pembuatan kecap dengan menggunakan ekstrak pepaya dan nanas dapat mempercepat waktu pembuatan kecap secara hidrolisis protein karena adanya enzim papain pada pepaya dan enzim bromelin pada nanas. Tujuan dari penelitian ini adalah untuk mengetahui kadar protein kecap biji turi dengan menggunakan ekstrak pepaya dan nanas serta untuk  mengetahui organoleptik kecap biji turi dengan menggunakan ekstrak pepaya dan nanas. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL pola faktorial. Faktor tersebut yaitu jenis ekstrak yang digunakan (ekstrak pepaya dan ekstrak nanas dan penambahan volume ekstrak (80 ml, 100 ml, dan 120 ml dengan 6 kombinasi perlakuan. Hasil penelitian menunjukkan bahwa penambahan ekstrak pepaya dan nanas berpengaruh pada kadar protein kecap. Hasil kadar protein tertinggi pada perlakuan J1V1 yaitu 12,11%,  sedangkan  kadar protein terendah pada perlakuan J2V1 yaitu 7,53 %. Kecap dengan perlakuan menggunakan ekstrak nanas, volume 120 ml merupakan kecap yang dapat diterima oleh masyarakat.

  16. Resultados preliminares do tratamento dos pacientes submetidos a radiofreqüência (baixa freqüência, somnoplastia no ronco e apnéia leve do sono

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    Dibbern Ralph S.

    2002-01-01

    Full Text Available Introdução: O ronco afeta 5 a 50% da população tornando-se um problema médico e social importante. O tratamento pode ser com medidas comportamentais, CPAP e cirúrgico. Objetivo: Avaliar os resultados pós-operatórios dos pacientes submetidos à radiofreqüência em palato. Forma de estudo: retrospectivo clínico. Material e método: 28 pacientes submetidos à radio-freqüência em palato, com diagnóstico de Ronco e Apnéia Leve do Sono. Observamos os resultados pós-operatórios através das Escalas de Epworth, Ronco e Sonolência diurna, complicações e percentual de melhora dos sintomas. A análise estatística foi pelo Mann Whytney Test. Resultados: 16 pacientes com melhora subjetiva do Ronco acima de 70% após a cirurgia; 26 pacientes mostraram redução significativa pelas Escalas, com complicações mínimas. Conclusão: Radio-freqüência é um método seguro e eficaz no tratamento do Ronco e Apnéia Leve do Sono. Resultados melhores serão obtidos com avaliação criteriosa pré e pós-operatórias.

  17. Renal scarrings (RS) after acute pyelonephritis (APN) in the children under the age of 5 years and its correlation with clinical risk factors

    Energy Technology Data Exchange (ETDEWEB)

    Lee, B. S.; Moon, D. H.; Ahn, S. H.; Lee, W. W.; Yoon, S. Y.; Yoon, J. H.; Park, Y. S. [College of Medicine, Ulsan Univ., Seoul (Korea, Republic of)

    2001-07-01

    In children with APN, risk factors for the subsequent RS are still controversial. We evaluated the changes in renal cortical defects on the Tc-99m DMSA scan and the relationship between RS and risk factors. Patients were 143 children (age < 5 yrs) with first APN documented by the presence of fever (< 38.5 .deg. C ), and abnormal DMSA scan from 1994 to 2000. We performed a follow-up DMSA scan at 6 months. RS was defined as persistent or partially reversible abnormalities on the follow-up scan. Nine were excluded due to recurrent infection, and 58 who were lost to follow-up. A total of 76 (m/f =38/38) were available for the study (mean age : 1.19 yr, range: 47 day - 5.0 yr). Of 152 kidneys, DMSA was abnormal in 90. Initial defect was completely recovered in 52, partially recovered in 30, and persistent in 8 (38 RS). Univariate analysis revealed that duration of fever, C-reactive protein, and vesicoureteral reflux (VUR) were associated with RS. Multivariate analysis showed that duration of fever and VUR were independent predictors of RS. However, in patients with unilateral defect, only duration of fever (p=0.027) and relative function (p=0.002) were independent predictors. The severity and extent of acute inflammatory response may determine RS. DMSA may be of value because it provides information on the extent of initial parenchymal damage.

  18. KANIBALISME PADA YUWANA IKAN KERAPU MACAN (Epinephelus fuscoguttatus DALAM KONDISI PEMELIHARAAN SECARA TERKONTROL

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    Eri Setiadi

    2006-08-01

    Full Text Available Ikan kerapu macan (Epinephelus fuscoguttatus merupakan ikan yang memiliki nilai ekonomi penting sebagai komoditas ekspor dan telah berhasil dibudidayakan di Indonesia. Kendala yang dihadapi dalam budi daya ikan tersebut yaitu masih tingginya tingkat kanibalisme pada pemeliharaan secara intensif. Tujuan penelitian ini adalah untuk mengetahui frekuensi gigitan, menelan, dan mortalitas pada pemeliharaan yuwana ikan kerapu macan dan upaya pengendaliannya. Penelitian ini terdiri atas dua tahap, yaitu penelitian variasi ukuran yang dipuasakan (Penelitian I dan variasi ukuran dengan kepadatan jembret yang berbeda (Penelitian II terhadap terjadinya kanibalisme, seperti frekuensi gigitan, menelan, dan mortalitas telah dilakukan. Ukuran yuwana kerapu macan yang digunakan sebagai hewan uji terdiri atas tiga ukuran, yaitu ukuran kecil, sedang, dan besar. Hasil penelitian I menunjukkan bahwa ada perbedaan (P<0,0001 di antara perlakuan terhadap frekuensi gigitan, menelan, dan mortalitas. Penelitian II menunjukkan juga adanya perbedaan (P<0,0006 di antara perlakuan terhadap frekuensi gigitan, menelan dan mortalitas. Kepadatan jembret 1.000 individu/L dapat mengurangi kanibalisme. Tiger grouper, E. fusoguttatus is one of the marine finfish species have a high economic value as an export commodity in Indonesia. Mass production of this species has largely been successful in Indonesia. However, cannibalism is a main problem can be reduced mass production during in an intensive culture system. The aim of this experiment is to examine frequency of bitting and swallowing, mortality, and its control. This experiment was focused on size variation and mysid density that affect on frequency of bitting, frequency of swallowing, and mortality. The fry of tiger grouper used in this experiment was consisted of three sizes (small, medium, and big. Two kinds of experiments were set up namely size variation and starvation (experiment I and size variation and mysid density

  19. TINJAUAN YURIDIS PENGAJUAN PEMBATALAN PERJANJIAN JUAL BELI SAHAM PERUSAHAAN SECARA SEPIHAK

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    Satriyo Wahyu Harsoyo

    2016-03-01

    Full Text Available Abstract Contract is an act by which one or more persons bind themselves to one person or more. Contract thus legally binding on the parties, to obtain rights or obligations specified in the contract. However, in a contract, not always run smoothly. As happened in filing a Share Purchase contract cancellation PT Kaltim Satria Samudera (PT KSS between the Sellers the Yayasan Kesejahteraan Hari Tua(YKHT with buyers Yayasan Pupuk Kaltim(YPK. Stock purchase plan agreement signed by PT KSS YPK and YKHT not include cancellation terms of the contract. The absence of a condition in the contract void makes negotiating the cancellation of the sale and purchase of shares in PT KSS be deadlock. This paper based on normative legal research. The results obtained indicate that based on the Theory of Legal System, the parties can resolve this disputeby means of negotiation and mediation. Retrieved alsoconcluded that the submission of cancellation of the contract can be done with the agreement of the parties or the cancellation of the contrac tto the court. Key words: contract, canceled, shares Abstrak Perjanjian adalah suatu perbuatan dengan mana satu orang atau lebih mengikatkan dirinya terhadap satu orang atau lebih. Perjanjian dengan demikian mengikat para pihak secara hukum, untuk mendapatkan hak atau melaksanakan kewajiban yang ditentukan dalam perjanjian itu. Namun dalam suatu perjanjian, tidak selamanya dapat berjalan dengan lancar. Seperti yang terjadi dalam Pengajuan pembatalan Perjanjian Jual Beli Saham PT Kaltim Satria Samudra (PT KSS antara Pihak Penjual yaitu Yayasan Kesejahteraan Hari Tua (YKHT dengan pembeli Yayasan Pupuk Kaltim (YPK. Kesepakatan rencana pembelian saham PT KSS yang ditandatangi oleh YPK dan YKHT tidak mencantumkan syarat pembatalan perjanjian tersebut. Ketiadaan syarat batal dalam perjanjian tersebut membuat negosiasi pembatalan jual beli saham PT KSS menjadi deadlock. Tulisan ini berdasarkan hasil penelitian hukum normatif

  20. 小菜蛾中肠氨肽酶N2在昆虫细胞中的表达%Expression of aminopeptidase N (APN2) from Plutella xylostella larval midgut in Sf9 cells

    Institute of Scientific and Technical Information of China (English)

    王俊华; 周小毛; 吴青君; 王少丽; 谢文; 陈得峰; 徐宝云; 张友军

    2012-01-01

    利用昆虫细胞-杆状病毒表达系统(Bac-to-Bac baculovirus expression system)表达获得小菜蛾Plutella xylostella(L.)中肠氨肽酶N2( aminopeptidase N,APN2)蛋白,成功建立了该蛋白在昆虫细胞中的表达体系.将对Cry1Ac毒素敏感和已产生抗性的小菜蛾中肠APN2基因插入表达载体pFAST Bac HTB中,并在草地夜蛾Spodoptera frugiperda细胞系(Sf9)中进行了重组表达.聚丙烯酰胺凝胶电泳( SDS-PAGE)及蛋白免疫印迹技术(Western-blotting)结果显示,在107 kDa处有1条特异性蛋白条带.研究表明,小菜蛾中肠APN2基因可在Sf9细胞中成功表达,这为继续研究其功能及抗性的关系奠定了基础.%Using the insect cells-Bac-to-Bac baculovirus expression system to express APN2 (aminopeptidase N) protein, the APN2 expression system in Sf9 cells was successfully established. APN2 gene from susceptible and resistant Plutella xylostella (L. ) larval midgut were inserted into pFAST Bac HTB vector, expressed in insect cells Spodoptera frugiperda (Sf9) cells, respectively, with Bac-to-Bac baculovirus expression system. A 107 kDa protein was examined and confirmed by SDS-PAGE and Western-blotting. The results showed that P. Xylostella larval midgut APN2 gene was successfully expressed in Sf9 cells which provided a foundation for further investigation on function of APN2 and its relationship with Bt resistance.

  1. ANALISIS CFD HAMBATAN LAMBUNG KAPAL TRIMARAN ASIMETRIS FLAT SIDE INSIDE DENGAN VARIASI JARAK ANTAR LAMBUNG SECARA MEMBUJUR

    Directory of Open Access Journals (Sweden)

    Andrew Gibson

    2016-04-01

    Full Text Available Kapal dibuat dengan fungsi sebagai sarana transportasi untuk kebutuhan niaga maupun perang. Saat ini kebutuhan kapal cepat meningkat sehingga diperlukan kapal dengan bentuk multihull, salah satunya adalah kapal Trimaran. Kapal Trimaran merupakan kapal yang mempunyai 3 lambung, yaitu satu mainhull dan dua side-hull atau disebut juga outriggers sehingga mempunyai nilai stabilitas yang tinggi. Untuk itu, perlu dilakukan penelitian lebih lanjut untuk membuat kapal Trimaran yang memiliki hambatan yang kecil sehingga dapat menambah nilai ekonomis dari segi efisiensi mesin dan performa kapal yang baik. Berbagai bentuk badan kapal kemudian dikembangkan untuk mendapatkan desain lambung kapal Trimaran yang paling optimum salah satunya adalah kapal Trimaran flat side inside, yaitu sebuah konfigurasi baru dengan lambung yang memiliki bidang datar atau asimetris di dalam. Penelitian ini bertujuan untuk mendapatkan variasi letak stagger atau jarak antar lambung secara membujur (R/L kapal Trimaran asimetris flat side inside yang menghasilkan hambatan paling kecil dengan menggunakan bantuan software Computational Fluid Dynamics (CFD. Dengan melakukan variasi kecepatan yaitu 12, 14, 16, dan 22 knot dan variasi jarak antar lambung (R/L dimana R merupakan jarak antar lambung secara membujur dan L adalah panjang kapal mainhull, yaitu untuk R/L = 0.1, R/L = 0.2 dan R/L = 0.3  pada ketentuan jarak antar lambung secara melintang (S/L = 0.4 dapat dilihat pengaruh interaksi gelombang terhadap nilai hambatan gelombang kapal Trimaran asimetris flat side inside. Hasil pengujian didapatkan bahwa  model C dengan R/L = 0,3 dan S/L = 0,4 adalah variasi model paling baik dalam tugas akhir ini karena menghasilkan hambatan viscous dan hambatan total        terkecil dengan presentase yang dibandingkan dengan model awal masing-masing 7,137 % dan 24,965 %.

  2. Dermatoglifia e composição corporal em apnéia obstrutiva do sono Dermatoglyphics and body composition in obstructive sleep apnea

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    Luiz Bittencourt Mercanti

    2004-09-01

    Full Text Available A síndrome da apnéia obstrutiva do sono (SAOS tem como principal fator de risco a obesidade e acredita-se que fatores genéticos poderiam contribuir na sua patogênese. O objetivo deste estudo foi descrever as características antropométricas e dermatoglíficas dos portadores de SAOS. Foram mensurados: indice de massa corporal (IMC; indice de conicidade, gordura corporal relativa, somatotipo e coletadas as impressões digitais. Trinta e um casos de SAOS foram comparados a número igual de controles. Pelo IMC e gordura corporal relativa os apnéicos foram classificados como obesos. O indice de conicidade revelou forte componente de obesidade central. No somatotipo, predominou a categoria endomorfo-mesomorfo, indicando alta magnitude dos componentes músculo-esquelético e adiposo com linearidade relativa de grande volume por unidade de altura. Para índices mais graves de apnéia observa-se maior predominância mesomórfica na composição corporal. Através do teste t, a dermatoglifia não mostrou diferenças estatisticamente significativas entre portadores da SAOS e os controles.Obesity is the main risk factor for obstructive sleep apnea syndrome (OSAS and genetic patterns can modulate the pathogenesis of the disease. The aim of this study is to describe the anthropometrics and dermatoglyphics features among OSAS carriers. We collected information on Body Mass Index (BMI, Conicity Index (CI, Body Fat Mass (BFM, somatotype and fingerprints. Thirty-one cases of OSAS were compared to an equal number of controls. Membership to the obese category is based on observed BMI and BFM. The CI distribution among cases shows a strong central obesity component. The endomorph-mesomorph somatotype category predominates among cases showing high adiposity and relative muscle-skeletic development, such as relative linearity of great mass per unit of height. Increased morbidity, as given by more serious indices of apnea, correlates positively with higher

  3. SINTESIS LAPIS TIPIS BERBASIS NANOPARTIKEL TITANIA TERMODIFIKASI SILIKA SECARA SOL-GEL SEBAGAI BAHAN ANTIFOGGING

    Directory of Open Access Journals (Sweden)

    L.V. Rissa

    2012-09-01

    Full Text Available Telah dilakukan penelitian tentang sintesis lapis tipis berbasis nanopartikel titania termodifikasi silika (TiO2-SiO2 secara sol-gel sebagai bahan antifogging untuk (i mengetahui pengaruh temperatur kalsinasi terhadap energi band-gap, struktur dan ukuran kristal, serta porositas titania-silika, (ii mengetahui pengaruh komposisi sol terhadap karakteristik lapis tipis titania-silika yang meliputi hidrofilitas dan sifat transparan, serta (iii mengetahui kinerja antifogging lapis tipis titania-silika pada substrat kaca. Sintesis dilakukan melalui  metode sol-gel. Teknik pelapisan pada kaca menggunakan teknik celup, kemudian diaplikasikan sebagai bahan anti-kabut. Hasil sintesis TiO2-SiO2 dikarakterisasi dengan XRD untuk mengetahui struktur dan ukuran kristal, SEM-EDX untuk mengetahui morfologi dan komposisi padatan, serta DR-UV untuk mengetahui band-gap TiO2-SiO2. Hasil karakterisasi menunjukkan bahwa TiO2-SiO2 dengan 15% b/b silika  pada temperatur kalsinasi 500°C memiliki ukuran partikel dan band gap optimum  yaitu masing-masing sebesar 11,15 nm dan  3,20 eV. Hasil ini bersesuaian dengan hasil pengukuran sudut kontak minimum yaitu sebesar sebesar 14,49°. Hasil uji kinerja antifogging menunjukkan bahwa lapis tipis TiO2-SiO2 memiliki kemampuan untuk digunakan sebagai bahan anti-kabut. The research on synthesis of thin layer Based on TiO2 nanoparticle modified silica by sol gel as antifogging materials has been conducted to (i determine the effect of calcination temperature on the gap energy, structure and size of crystal, and porous titania-silica, (ii determine the effect of sol composition on thin layer titania-silica covering hydrophilic and transparent character, and (iii determine the performance of antifogging thin layer titania-silica on glass substrate. The synthesis is done by using sol-gel method. Dye technique was used to coat the glass, and then applied as antifogging materials. The result of synthesis of TiO2-SiO2

  4. SINTESIS LAPIS TIPIS BERBASIS NANOPARTIKEL TITANIA TERMODIFIKASI SILIKA SECARA SOL-GEL SEBAGAI BAHAN ANTIFOGGING

    Directory of Open Access Journals (Sweden)

    L.V. Rissa

    2012-09-01

    Full Text Available Telah dilakukan penelitian tentang sintesis lapis tipis berbasis nanopartikel titania termodifikasi silika (TiO2-SiO2 secara sol-gel sebagai bahan antifogging untuk (i mengetahui pengaruh temperatur kalsinasi terhadap energi band-gap, struktur dan ukuran kristal, serta porositas titania-silika, (ii mengetahui pengaruh komposisi sol terhadap karakteristik lapis tipis titania-silika yang meliputi hidrofilitas dan sifat transparan, serta (iii mengetahui kinerja antifogging lapis tipis titania-silika pada substrat kaca. Sintesis dilakukan melalui  metode sol-gel. Teknik pelapisan pada kaca menggunakan teknik celup, kemudian diaplikasikan sebagai bahan anti-kabut. Hasil sintesis TiO2-SiO2 dikarakterisasi dengan XRD untuk mengetahui struktur dan ukuran kristal, SEM-EDX untuk mengetahui morfologi dan komposisi padatan, serta DR-UV untuk mengetahui band-gap TiO2-SiO2. Hasil karakterisasi menunjukkan bahwa TiO2-SiO2 dengan 15% b/b silika  pada temperatur kalsinasi 500°C memiliki ukuran partikel dan band gap optimum  yaitu masing-masing sebesar 11,15 nm dan  3,20 eV. Hasil ini bersesuaian dengan hasil pengukuran sudut kontak minimum yaitu sebesar sebesar 14,49°. Hasil uji kinerja antifogging menunjukkan bahwa lapis tipis TiO2-SiO2 memiliki kemampuan untuk digunakan sebagai bahan anti-kabut. The research on synthesis of thin layer Based on TiO2 nanoparticle modified silica by sol gel as antifogging materials has been conducted to (i determine the effect of calcination temperature on the gap energy, structure and size of crystal, and porous titania-silica, (ii determine the effect of sol composition on thin layer titania-silica covering hydrophilic and transparent character, and (iii determine the performance of antifogging thin layer titania-silica on glass substrate. The synthesis is done by using sol-gel method. Dye technique was used to coat the glass, and then applied as antifogging materials. The result of synthesis of TiO2-SiO2

  5. Upaya Peningkatan Kualitas Pembelajaran Bahasa Inggris secara Komunikastif dengan Teknik Picture Cue Cards di SMK Negeri 3 Purwokerto

    Directory of Open Access Journals (Sweden)

    Endar Yuniarti

    2004-06-01

    Full Text Available Penelitian ini dilakukan sebagai upaya untuk meningkat-kan kualitas pembelajaran bahasa Inggris secara komunikatif dengan teknik Picture Cue Cards (PCQ dan prestasi hasil belajar siswa. Penelitian tindakan kelas ini dilaksanakan di SMK Negeri 3 Purwokerto kelas II jurusan Akomodasi Perhotelan (AF tahun akademik 2002/2003. Kelas II AP dipilih berdasarkan prasurvei bahwa kualitas proses pembelajaran pada kelas II AP rendah serta kemampuan berkomunikasi siswa dalam bahasa Inggris dan nilainya tidak menggembirakan. Tindakan kelas dilaksanakan dalam tiga siklus. Data dan informasi tentang peningkatan kualitas pembelajar-an bahasa Inggris secara komunikatif dengan teknik PCC ini diperoleh melalui tes hasil belajar, kaji dokumentasi, observasi, wawancara, angket, dan catatan lapangan. Hasil penelitian menunjukkan bahwa aktivitas siswa selama proses pembelajaran meningkat Siswa juga menjadi lebih perhatian, berani berpendapat, dan aktif. Di samping itu, sikap afektif siswa juga meningkat, terbukti dalam menggalang kerja sama kelompok lebih menghargai pendapat orang lain, saling membantu, dan disiplin. Penguasaan materi menjadi lebih baik dan prestasi hasil belajarnya meningkat daripada sebelum diberikan perlakuan. Empat keterampilan berbahasa siswa, yaitu menyimak, berbicara, membaca, dan menulis juga meningkat Kata kunci: pembelajaran babasa inggris, teknik picture cue cards.

  6. FENOMENA KOROSI ZIRKALOY-2 DAN ZIRKALOY-4 DALAM MEDIA LARUTAN NaCl SECARA ELEKTROKIMIA

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    Dian Anggraini

    2016-03-01

    Full Text Available ABSTRAK FENOMENA KOROSI ZIRKALOY-2 DAN ZIRKALOY-4 DALAM MEDIA LARUTAN NaCl SECARA ELEKTROKIMIA. Zirkaloy-2 dan zirkaloy-4 digunakan sebagai bahan kelongsong pada elemen bahan bakar reaktor daya BWR (Boiling Water Reactor dan PWR (Pressurized Water Reactor. Selama penggunaanya dalam kolam penyimpanan bahan bakar bekas kemungkinan kelongsong berinteraksi dengan air laut (sea water, dalam hal kondisi abnormal. Ion klorida yang terdapat dalam air laut memiliki potensi penyebab terjadinya korosi pada bahan kelongsong. Tujuan penelitian ini adalah untuk mengetahui fenomena korosi zirkaloy-2 dan zirkaloy-4 dalam media NaCl melalui penentuan parameter korosi dengan teknik tahanan polarisasi, Tafel dan potensiodinamik. Sampel berupa potongan bahan zirkaloy-2 dan zirkaloy-4 dengan dimensi 1x1 cm dilakukan proses mounting dan disolder dengan kawat tembaga, kemudian permukaan sampel dipoles menggunakan amplas 1200 grit. Uji korosi dilakukan dalam sel korosi yang dilengkapi dengan elektrode standar (saturated calomel, elektroda penyangga (grafit dan elektroda kerja (sampel. Media pelarut yang digunakan adalah larutan NaCl dengan konsentrasi 3,5  %; 0,35 % dan 0,175 %. Hasil penelitian menunjukkan bahwa adanya ion Clˉ pada daerah konsentrasi NaCl 0,175 % sampai dengan 3,5 % tidak mempengaruhi pola mekanisme korosi. Jenis korosi pada daerah potensial ± 250 mV terhadap Ecorr adalah korosi merata dalam bentuk oksida ZrO2. Laju korosi zirkaloy-2 dan zirkaloy-4 pada konsentrasi NaCl 3,5 % diperoleh masing-masing sebesar 6,39×10-3 dan 7,40×10-3 mpy. Fenomena korosi yang diamati dengan teknik potensiodinamik (± 1000 mV terhadap Ecorr menunjukkan bahwa zirkaloy-2 dan zirkaloy-4 mengalami pasivasi dan korosi pitting. Potensial pitting zirkaloy-2 dan zirkaloy-4 diperoleh masing- masing sebesar -452,8 mV dan -182,8 mV. Kata kunci: Korosi, zirkaloy-2 dan zirkaloy-4, media larutan NaCl, elektrokimia, teknik polarisasi ABSTRACT CORROSION PHENOMENA OF ZIRKALOY-2 AND

  7. Daya Hambat Ekstrak Daun Pegagan (Centella asiatica yang Diambil di Batusangkar terhadap Pertumbuhan Kuman Vibrio cholerae secara In Vitro

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    Nelvita Sari Ramadhan

    2015-01-01

    Full Text Available AbstrakPegagan (Centella asiatica merupakan salah satu tanaman yang digunakan sebagai obat. Salah satu manfaat yang bisa didapatkan dari pegagan (Centella asiatica adalah antibakterinya. Manfaat antibakterinya didapatkan karena pegagan (Centella asiatica mengandung zat antibakteri, diantaranya adalah saponin, tannin, alkaloid, dan flavonoid. Telah dilakukan penelitian tentang daya hambat ekstrak pegagan (Centella asiatica terhadap pertumbuhan Vibrio cholerae secara in vitro, dengan tujuan untuk mengetahui daya hambat ekstrak pegagan (Centella asiatica terhadap pertumbuhan Vibrio cholera secara in vitro. Penelitian dilakukan dengan metode eksperimental laboratorium dengan metode difusi (cakram, pada berbagai konsentrasi yaitu 5%, 10%, 20%, 30%, 40%, 50%, dan100%, di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Hasil penelitian didapatkan bahwa ekstrak daun pegagan (Centella asiatica yang diambil di daerah Batusangkar, ternyata tidak dapat menghambat pertumbuhan kuman Vibrio cholerae secara in vitro, sedangkan tetrasiklin yang digunakan sebagai kontrol positif memberikan daya hambat yang baik terhadap pertumbuhan Vibrio cholera, dengan zona hambat 16,3 mm. Ada atau tidaknya daya hambat ekstrak pegagan (Centella asiatica terhadap pertumbuhan Vibrio cholerae dalam penelitian ini bisa dipengaruhi oleh jenis bakteri yang digunakan, metode pembuatan ekstrak yang dipakai, dan sumber daun pegagan yang digunakan dalam penelitian.Kata kunci: efek antibakteri, ekstrak daun pegagan (Centella asiatica, Vibrio choleraeAbstractCentella asiatica is one of the plants used as medicine. One of the benefits that can be obtained from Centella asiatica is an antibacterial effect. Antibacterial effect obtained as Centella asiatica contains anti-bacterial substances, such as saponins, tannins, alkaloids, and flavonoids. This study was conducted to determine the inhibition of extracts of Centella asiatica on the growth of Vibrio cholerae in vitro

  8. PENERAPAN PEMBELAJARAN DENGAN PENDIDIKAN MATEMATIKA REALISTIK (PMR SECARA BERKELOMPOK UNTUK MENINGKATKAN KEMAMPUAN PEMECAHAN MASALAH MATEMATIS SISWA DI KELAS X SMA

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    Atik Krismiati

    2013-09-01

    Full Text Available Tujuan dari penelitian ini adalah untuk mengetahui peningkatan kemampuan pemecahan masalah matematis serta kinerja siswa. Subyek populasi dalam penelitian ini adalah siswa SMA Aloysius Bandung. Pendekatan yang digunakan dengan PMR. Instrumen yang digunakan terdiri dari: tes kemampuan pemecahan masalah dan aktivitas siswa selama pembelajaran. Secara keseluruhan siswa yang pembelajaran pemecahan masalah dengan metode PMR lebih baik dalam meningkatkan kemampuan pemecahan masalah, yaitu terlihat dengan adanya peningkatan dari siklus I ke siklus II. Kesulitan siswa terutama pada permasalahan dengan aspek argumentasi dan keakuratan. Selain itu kelebihan dari metode ini siswa lebih terlihat menyukai yaitu terlihat dengan antusiasnya mengerjakan tugas-tugas dari guru serta memberi alasan secara geometri, kreativitas, dan generalisasi yang sebagian besar perwujudannya dilakukan oleh siswa sendiri. Berdasarkan respon dan hasil akhir LKS menunjukkan aktivitas, dan kinerja yang lebih meningkatkan untuk setiap siklusnya Kata Kunci : Pendidikan Matematika Realistik, Pemecahan Masalah Matematis     The purpose of this study is to determine the increase in mathematical problem-solving skills as the well as student performance. The population subjects in this study are the high school students Aloysius Bandung . The approach applied is PMR . The used instruments consisted of : problem solving ability testing and students activities during the learning process. Overall, the students who are applying PMR method is better, which is seen from the increasing cycle I to cycle II. The primary students difficulties are on problems with aspects of argumentation and accuracy. However the advantages of this method the students look more enthusiastic in doing the tasks given are by the teachers, and they are also able to give geometrical and creative reasons. Which most of its manifestations are generally made by the students themselves. Based on the response and the

  9. Ekspresi protein vimetin dan neurofilamen pada tunas anggota depan mencit black-6 umur kebuntingan 12 hari akibat induksi 2-metoksietanol secara Real Time RT-PCR

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    YULIA IRNIDAYANTI

    2011-11-01

    Full Text Available Irnidayanti Y. 2011. Ekspresi protein vimetin dan neurofilamen pada tunas anggota depan mencit black-6 umur kebuntingan 12 hari akibat induksi 2-metoksietanol secara Real Time RT-PCR. Bioteknologi 8: 53-58. Tujuan penelitian ini adalah untuk mengetahui dampak dari 2-metoksietanol, bahan kimia utama industri plastik. Analisis ekspresi gen semakin penting dalam penelitian biologi, dimana real-time reverse transcription PCR (RT-PCR menjadi metode yang dipilih untuk mendapatkan profil ekspresi dari seleksi gen secara akurat. Mencit black-6 bunting diinjeksi 2-metoksietanol secara intraperitoneal dengan 7,5 mmol/kg pada umur kebuntingan 100 hari. Embrio diperoleh pada umur kebuntingan 12 hari. Tunas anggota depan embrio dikumpulkan dan dimasukkan ke dalam tabung, yang mengandung larutan RNA-latter. Untuk mengetahui perubahan ekspresi gen tunas anggota depan yang disebabkan oleh induksi 2-metoksietanol digunakaan RT-PCR dalam penelitiaan ini. Dalam percobaan digunakan teknologi RT-PCR Li ght Cycler. Hasil penelitiaan menunjukkan bahwa injeksi 2-metoksietanol, pada periode pralahir terutama pada umur kebuntingan 12 hari, ekspresi vimentin pada tunas anggota depan mencit perlakuan meningkatkan dibandingkan kontrol. Sementara itu ekspresi neurofilamen cenderung menurun, secara tidak langsung tidak disebabkan oleh injeksi 2-metoksietanol.

  10. Síndrome da apnéia-hipopnéia obstrutiva do sono. Fisiopatologia Physiopathology of obstructive sleep apnea-hypopnea syndrome

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    Andrea Barral Martins

    2007-02-01

    Full Text Available A fisiopatogenia da apnéia obstrutiva do sono é multifatorial. O sexo, a obesidade, os fatores genéticos, anatômicos e hormonais e o controle da ventilação interagem diversamente na fisiopatogenia e expressão clínica da doença. A obesidade é o principal fator de risco, sendo a elevação do índice de massa corpórea, da gordura visceral e da circunferência do pescoço, fortes preditores de sua ocorrência. A progesterona, por aumentar a atividade dos músculos dilatadores das vias aéreas superiores, tem papel protetor nas mulheres antes da menopausa, justificando a maior prevalência da doença na pós-menopausa, no sexo masculino e na síndrome dos ovários policísticos. Evidências apontam para o fato de que o aumento da idade promove diminuição do tônus muscular, com redução da luz das vias aéreas superiores. O dismorfismo crânio-facial, como na retrognatia ou micrognatia, está associado ao posicionamento posterior da língua, e pode resultar em estreitamento da luz das vias aéreas superiores. Finalmente, comando ventilatório reduzido tem sido detectado em pacientes com síndrome de apnéia obstrutiva do sono e hipercapnia.The physiopathology of obstructive sleep apnea syndrome is multifactorial. Gender and obesity status, as well as genetic, anatomic, and hormonal factors, together with ventilatory drive, interact in a diverse manner in the physiopathology and clinical expression of the disease. Obesity is the main risk factor, since increases in body mass index, visceral fat, and neck circumference are strong predictors of the disease. Progesterone increases the activity of the upper airway dilator muscles and therefore plays a protective role in premenopausal women. This explains the fact that the prevalence of the disease is higher in postmenopausal patients, in patients with polycystic ovary syndrome, as well as in males. Evidence supports the fact that, as individuals grow older, there is a decrease in muscle

  11. Estresse oxidativo hepático no modelo animal de apnéia do sono : efeitos de diferentes tempos de exposição à hipóxia intermitente

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    Darlan Pase da Rosa

    2010-01-01

    Síndrome da apnéia obstrutiva do sono (SAOS) é uma doença crônica comum com importantes consequências cardiovasculares e neuropsiquiátricas. Repetidos eventos de apnéia causam hipóxia intermitente (HI) com alteração de função em diferentes sistemas. Na SAOS ocorre períodos de isquemia e de reperfusão, com isso a formação de radicais livres e estresse oxidativo (EO). Diferentes estudos experimentais na SAOS descrevem o envolvimento do EO e como conseqüência dano hepático entre outros, porem nã...

  12. Estudo comparativo entre análise cefalométrica radiográfica e exame polissonográfico para diagnóstico da síndrome da apnéia obstrutiva do sono

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    Silva, Emerson Luiz Costa da

    2001-01-01

    O objetivo deste estudo foi comparar a eficiência do exame cefalométrico radiográfico em relação ao exame polissonográfico para o diagnóstico da Síndrome da Apnéia Obstrutiva do Sono (SAOS), em 60 pacientes com suspeita clínica da condição. Observou-se a confiabilidade dos dados obtidos na cefalometria, vez que esta vem sendo utilizada largamente no protocolo de exame de pacientes apnêicos, principalmente para a determinação dos sítios obstrutivos, servindo desta forma de guia terapêutico. Os...

  13. MODEL PERPINDAHAN MASSA PADA EKSTRAKSI SAPONIN BIJI TEH DENGAN PELARUT ISOPROPIL ALKHOHOL 50% DENGAN PENGONTAKAN SECARA DISPERSI MENGGUNAKAN ANALISIS DIMENSI

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    Susiana Prasetyo

    2012-11-01

    Full Text Available Indonesia merupakan salah satu penghasil teh terbesar di dunia. Menurut data dari Deptan 2010, dengan luas lahan sebesar 127712 ha, Indonesia dapat menghasilkan produktivitas teh sebesar 153971 ton/tahun. Namun sayangnya, selama ini pemanfaatan tanaman teh di Indonesia masih terbatas pada pucuk daunnya saja. Bagian tanaman teh yang memiliki kandungan potensial namun belum dimanfaatkan adalah  biji teh, mengandung 26% saponin, 20-60% minyak dan 11% protein. Saponin merupakan salah satu komponen bioaktif yang telah dimanfaatkan sebagai insektisida pembasmi hama pada tambak udang, bahan baku industri deterjen, shampoo, minuman bir, pembentuk busa pada pemadam kebakaran, dan dapat dimanfaatkan pula sebagai pupuk organik. Pada penelitian ini, saponin biji teh diperoleh melalui ekstraksi biji teh pasca pengepresan menggunakan pelarut IPA 50% secara batch di dalam sebuah ekstraktor berkapasitas 2 L. Pengontakan solvent dengan biji teh dilakukan secara dispersi. Pada penelitian ini, rasio massa pelarut terhadap massa umpan diset 20:1; temperatur divariasikan 25-60 oC, kecepatan pengadukan 100-400 rpm, serta ukuran biji teh divariasikan -40+50 mesh s.d -100+200 mesh. Ekstraksi dilakukan hingga tercapai kesetimbangan. Ekstrak yang diperoleh dipekatkan menggunakan rotavapor vakum pada temperatur di bawah 40oC. Ekstrak pekat saponin kasar yang didapat dimurnikan dengan penambahan eter, etanol dan petroleum eter secara bertahap sehingga diperoleh endapan saponin murni yang kemudian dikeringkan menggunakan tray drier pada temperatur 40oC. Pada penelitian ini, model perpindahan massa sederhana dikembangkan untuk menggambarkan proses ekstraksi padat-cair saponin biji teh. Berdasarkan hasil analisis dimensi diperoleh hubungan antara koefisien perpindahan massa volumetrik (kLa pada lapisan antar fasa padat-cair dengan variabel-variabel ekstraksi yang dinyatakan dalam persamaan bilangan tak berdimensi dengan ralat rata-rata 3,7904 %.DIMENSIONAL ANALYSIS FOR MASS

  14. Expression of aminopeptidase N (APN on peripheral blood mononuclear cells' surface as a marker of these cells' transendothelial migration properties in the course of multiple sclerosis

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    J. Ziąber

    2000-01-01

    Full Text Available CD13 AG and CD11a, CD11b, CD18 molecule expression on peripheral blood mononuclear cells (PBMC were studied as these cells’ adherent or transendothelial migration properties in three different multiple sclerosis (MS patients groups (total 38: with clinically active MS (acute exacerbation of MS and primary chronic progressive MS (CP-MS and with MS remission. The control group consisted of patients, suffering from other non-inflammatory neurological diseases (OND. The results of our study suggest that CD11a/CD18 molecules expression on PB lymphocytes, although higher on these cells’ surface in the course of MS as compared to OND, does not differentiate clinical forms of MS. CD11a molecule expression on monocytes did not differ significantly in all tested MS patient groups in comparison to OND. Although the expression of CD11b/CD18 molecules on monocytes’ surface shows their activation in the course of MS, it does not differentiate them either. However, CD13 Ag of APN expression on PBMC surface may be an immunological marker of MS clinical form. CD13 Ag expression may also be a sensitive marker of these cells’ transendothelial migration properties.

  15. Uji Daya Hambat Ekstrak Buah Belimbing Manis (Averrhoa carambola terhadap Pertumbuhan Bakteri Streptococcus pneumoniae secara In Vitro

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    Rita Risandi

    2016-09-01

    Full Text Available AbstrakBuah belimbing manis (Averrhoa carambola merupakan salah satu tanaman Indonesia yang diyakini memiliki khasiat obat. Salah satu manfaat yang dapat diambil dari sari buah belimbing manis (Averrhoa carambola adalah dapat mengobati radang tenggorokan. Radang tenggorokan merupakan salah satu infeksi yang disebabkan oleh bakteri Streptococcus pneumoniae. Tujuan penelitian ini adalah menentukan daya hambat ekstrak buah belimbing manis (Averrhoa carambola terhadap pertumbuhan bakteri Streptococcus pneumoniae  secara in vitro. Metode studi ini ialah eksperimental dengan desain postest only control group design yang dilakukan di Laboratorium Biota Sumatera Universitas Andalas dan Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas dari Agustus sampai Oktober 2014. Hasil penelitian menunjukkan bahwa ekstrak buah belimbing manis (Averrhoa carambola dengan konsentrasi yaitu 5%, 10%, 15% dan 20% tidak memiliki daya hambat terhadap pertumbuhan bakteri Streptococcus pneumoniae.  Hal ini terbukti karena tidak terbentuk zona hambat pada agar darah dan tidak terdapat pengaruh lama kontak ekstrak buah belimbing manis (Averrhoa carambola  terhadap pertumbuhan bakteri Streptococcus pneumoniae secara in vitro. Ekstrak buah belimbing manis tidak memiliki efek antibakteri terhadap pertumbuhan bakteri Streptococcus pneumoniae.Kata kunci: ekstrak buah belimbing manis, Streptococcus pneumoniae, daya hambat Abstract             Star fruit (Averrhoa carambola is a Indonesian plant that is believed to have medicinal properties. One of the benefits that can be drawn from the juice of star fruit (Averrhoa carambola is the ability to treat strep throat. Strep throat is a bacterial infection caused by Streptococcus pneumoniae. The objective of this study was to determine the inhibitory extract of star fruit (Averrhoa carambola on the growth of the bacterium Streptococcus pneumoniae in vitro. This was an experimental  research  with design

  16. Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro

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    Septriana Putri

    2015-09-01

    Full Text Available Abstrak Candida albicansb (C. albicans adalah salah satu mikroorganisme penyebab masalah kesehatan reproduksi wanita, yaitu keputihan (fluor albus. Penggunaan larutan povidone iodine dan bahan alam seperti ekstrak daun sirih menjadi pilihan masyarakat sebagai pembersih alat kewanitaan. Tujuan penelitian ini adalah untuk membandingkandaya hambat larutan antiseptik povidone iodine dan ekstrak daun sirih terhadap jamur C. albicans secara in vitro. Penelitian dilakukan terhadap lima isolat jamur C. albicans dengan larutan kontrol akuades.Perlakuan terdiri dari povidone iodine, ekstrak daun sirih dengan konsentrasi 5%, 10%, dan 20%.Hasil penelitian menunjukkan bahwa povidone iodine memiliki daya hambat terhadap C. albicans. Ekstrak daun sirih dengan konsentrasi 5% dan 10% tidak memiliki daya hambat terhadap C. albicans, namun ekstrak daun sirih konsentrasi 20% memiliki daya hambat terhadap C. albicans. Analisis statistik dengan uji ANOVA yang dilanjutkan dengan uji Post-hoc menunjukkan perbedaan bermakna antara daya hambat larutan povidone iodine dan ekstrak daun sirih 20% terhadap kontrol(p < 0.05.Larutan povidone iodine memiliki daya hambat dua kali lebih besar terhadap pertumbuhan C. albicans dibandingkan ekstrak daun sirih 20%. Dari penelitian ini dapat disimpulkan bahwa larutan povidone iodine dan ekstrak daun sirih 20% dapat menghambat pertumbuhan jamur C. albicans secara in vitro. Kata kunci: povidone iodine, ekstrak daun sirih, Candida albicansAbstract Candida albicans (C. albicans is one of the frequent causes of  reproductive health problems in women, namely vaginal discharge (fluor albus. The antiseptic solution, povidone iodine, is still an option to overcome vaginal discharge. The use of natural materials such as betel (Piper betle L. leaves extract also become a popular choice as adouche for women. The objective of this study was to compare the inhibitory activity of povidone iodine solution and betel leaf extract against the growth of

  17. Uji Efektivitas Pestisida terhadap Beberapa Patogen Penyebab Penyakit Penting pada Buah Naga (Hylocereus sp. secara In Vitro

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    Ani Widiastuti

    2011-12-01

    Full Text Available Problem caused by pathogen in Dragon Fruit (Hylocereus sp. cultivation became very important because it decreased significantly the quantity and quality of the fruit production. This study aims to determine the effectiveness of some pesticides against pathogens that cause diseases of dragon fruit in several plantations in the DIY (Sleman and Kulon Progo and Central Java (Magelang and Batang. Test of fungicide effectiveness was done in vitro on PDA medium (potato dextrose agar by poisoned food technique. The fungicides were mancozeb 80 %, methyl tiofanat 70 %, copper hydroxide 80 %, chlorotalonil 75 %, mancozeb 64 % + metalaxyl 4 %, mancozeb karbendazim 73.8 % + 6.8 %,benomyl 50 % at a concentration of 1g/L. The bactericides used were streptomycin sulfate 20 % and oxytetracycline 150 AL with each concentration of 1 g/L and 1 mL /L. The results showed that benomyl 50 % was the most effective fungicide to suppress the growth of Fusarium sp. (brown spot, Colletotrichum sp. (anthracnose and Pestalotiopsis sp. (scab, followed by mancozeb 73.8 % +karbendazim 6.8 % and 73.8 % mancozeb. Bactericide which was able to suppress the growth of Erwinia sp. (stem rot was streptomycin sulfate 20 %.   Gangguan patogen pada buah naga (Hylocereus sp. saat ini menjadi masalah penting karena secara signifikan menurunkan kuantitas dan kualitas hasil panen. Penelitian ini bertujuan untuk mengetahui keefektivan beberapa pestisida terhadap patogen penyebab penyakit-penyakit penting pada tanaman buah naga yang ditanaman pada beberapa sentra pertanaman di Propinsi DIY (Sleman dan Kulon Progo dan Jawa Tengah (Magelang dan Batang. Uji keefektivan pestisida dilakukan secara in vitro pada medium PDA (potato dextrose agar dengan metode teknik makanan beracun (poisoned food technique. Fungisida yang dipergunakan adalah mankozeb 80%, tiofanat metil 70%, tembaga hidroksida 80%, klorotalonil 75%, mankozeb 64% + metalaksil 4%, mankozeb 73,8% + karbendazim 6,8%, benomil 50% dengan

  18. ANALISIS DIAZINON SECARA DIFERENSIAL PULSA VOLTAMETRI DIBANDINGKAN DENGAN KROMATOGRAFI (Diazinon Analysis with Differential Pulse Voltametry Compared to Chromatography

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    Pirim Setiarso

    2011-07-01

    Full Text Available ABSTRAK Telah dilakukan penelitian penentuan diazinon dari sampel tanah pertanian di beberapa kecamatan Kabupaten Nganjuk Jawa Timur menggunakan elektroda kerja tembaga amalgam padat (CuSAE secara diferensial pulsa voltametri (DPV dibandingkan dengan metode kromatografi. 100 g sampel tanah pertanian yang dihaluskan dengan ukuran 100 mesh diekstraksi menggunakan aseton. Ekstraksi dilakukan menggunakan ekstraktror soxhlet dengan 10 kali sirkulasi. Hasil ekstraksi sebagian ditambahkan surfaktan SDS untuk dianalisis secara voltametri menggunakan elektroda CuSAE dan sebagian dianalisis menggunakan kromatografi cair kinerja tinggi (HPLC. Analisis voltametri dilakukan pada pH 3 dengan elektrolit pendukung CaCl 2 menggunakan standar adisi, sedangkan analisis dengan HPLC menggunakan grafik standar. Berdasarkan data voltamogram dan kromatogram yang telah diolah dengan program origin 7.0 menghasilkan perolehan kembali rata-rata diazinon (97.40 ± 1.02 % pada voltametri dan (100.98 ± 7.10 % pada HPLC. Analisis diazinon kedua metode dibandingkan dengan uji t (t hitung = 0.4785 < t tabel = 2.31 sehingga tidak ada perbedaan hasil antara metode voltametri dan HPLC. ABSTRACT The research has been done for determination of diazinon in agricultural soil samples from several districts n Nganjuk, East Java . Solid copper amalgam (CuSAE used  as a working electrode using differential ulse voltammetry (DPV compared with chromatographic method. Around 100 g sample of agricultural soil ith a size 100 mesh was extracted using 100 ml acetone. The extraction was done using extractor oxhlet with 10 times the circulation. A part of the extraction  yield  was  added SDS surfactant for analysis by DPV,  and another part analyzed  by using high performance liquid chromatography (HPLC. Voltammetric analysis carried out at pH 3 with CaCl2 as a supporting electrolyte   using standard addition method, while the analysis by HPLC using calibration curve (standard

  19. Gambaran Darah Anjing Yang Diinjeksi Xilasin-Ketamin Secara Subkutan (DESCRIPTION BLOOD OF DOG XYLAZINE-KETAMINE INJECTED SUBCUTANEOUSLY

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    Rosni Lumban Gaol

    2016-02-01

    Full Text Available Penelitian ini bertujuan mengetahui pengaruh injeksi berulang xilasin-ketamin melalui sub kutan terhadap total eritrosit, kadar hemoglobin dan hematokrit anjing lokal. Sebanyak 24 ekor anjing lokal umur 1-2 tahun berat badan 10-15 kg sebagai hewan coba. Anjing dikelompokkan  menjadi tiga. Kelompok pertama sebagai kontrol, sedangkan kelompok kedua diinjeksi kombinasi xilasin-ketamin dengan dosis dua dan 10 mg/kg bb dan kelompok ketiga diinjeksi dengan dosis xilasin (2 mg/kg bb dan ketamin dosis (10 mg/kg bb secara sub kutan. Darah diambil sebelum anestesi dan sesudah masa pemulihan anestesi melalui vena femoralis. Pemeriksaan eritrosit dengan Neubauer, pemeriksaan kadar hemoglobin dengan metode Sahli dan pemeriksaan hematokrit dengan metode Microhematocrit. Hasil penelitian menunjukkan bahwa tidak terjadi perbedaan yang nyata terhadap total eritrosit, kadar hemoglobin dan hematokrit pada perlakuan injeksi berulang xilasin-ketamin yang dikombinasi ataupun dipisah dengan kelompok kontrol. Dari hasil tersebut dapat disimpulkan bahwa injeksi berulang xilasin-ketamin dengan dosis xilasin (2 mg/kg bb dan ketamin (10 mg/kg bb spesifikasinya aman terhadap total eritrosit, kadar hemoglobin dan hematokrit dan baik digunakan sebagai anestesi pada anjing.

  20. PERANCANGAN APLIKASI UJIAN SARINGAN MASUK PERGURUAN TINGGI SECARA ONLINE BERBASIS ANDROID (STUDI KASUS UNIVERSITAS POTENSI UTAMA MEDAN

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    Deny Adhar

    2016-10-01

    Full Text Available Perkembangan teknologi informasi telah menyentuh hampir semua sektor. Hal ini menuntut peningkatkan kinerja baik dari segi efektifitas dan efesiensi. Salah satu sektor yang menjadi hal penting untuk diperhatikan peningkatan kinerja ialah sektor pendidikan. Dengan meningkat dan berkembangnya teknologi informasi dibutuhkan suatu sistem pendidikan yang berkualitas baik dari segi mutu materi pendidikan, pengajaran, pengujian serta sarana dan prasarana pendidikan. Dalam hal ini yang menjadi sorotan yang baik untuk dijadikan pembahasan ialah sistem pengujian dimana masih banyak lembaga – lembaga pendidikan baik universitas, sekolah-sekolah dan lembaga pendidikan non-formal lainnya yang menggunakan cara manual dalam melakukan ujian saringan masuk. Penggunaan cara manual ini banyak mengalami kendala dalam pelaksanaannya, diantaranya dalam hal biaya penyediaan bahan soal dan jawaban ujian, waktu pengerjaan dan sebagainya. Penelitian ini bertujuan untuk memanfaatkan kemajuan teknologi iternet berbasis mobile, perangkat mobile seperti handphone sekarang layaknya seperti komputer dapat diinstal berbagai macam aplikasi. Sistem ujian saringan masuk secara online ini diimplementasikan dalam sistem operasi mobile yaitu android yang sedang berkembang pesat saat ini, sehingga semua kendala yang ditemui pada saat menjalankan cara manual dapat diperkecil atau bahkan dihilangkan. Hasil penelitian ini dapat digunakan sebagai rekomendasi peningkatan pelayanan dalam perguruan tinggi. Kata kunci— Sistem Informasi, Ujian Saringan Masuk, aplikasi Android, Perguruan Tinggi

  1. PENANGGULANGAN PENANGKAPAN IKAN SECARA TIDAK SAH (ILLEGAL FISHING OLEH KAPAL IKAN ASING DI ZONA EKONOMI EKSKLUSIF INDONESIA

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    Melly Aida

    2014-03-01

    Full Text Available Penangkapan ikan secara tidak sah ( illegal fishing oleh kapal ikan asing di wilayah Zona Ekonomi Eksklusif Indonesia ( ZEEI semakin tahun semakin meningkat. Seiring dengan kondisi tersebut negara menderita kerugian yang besar dari tindakan ini. Ada anggapan bahwa terjadinya hal tersebut karena kewenangan penegakan hukum oleh negara pantai  ( Indonesia yang terbatas disebabkan  ketentuan yang ada di dalam Konvensi Hukum Laut 1982 ( KHL 1982 . Kemudian jika  dilihat dari segi penegakan hukum oleh pemerintah, penanganan illegal fishing nampaknya masih memprihatinkan. Tulisan ini ingin mengkaji bagaimana kewenangan Indonesia dalam menanggulangi illegal fishing di wilayah ZEEI berdasar KHL 1982. Selain itu dalam tulisan ini juga ingin dikaji bagaimana penanggulangan yang dilakukan oleh pemerintah Indonesia terhadap tindak illegal fishing ini. Data dalam tulisan ini dicari dengan cara melakukan studi kepustakaan, artinya dengan cara mempelajari buku-buku, naskah-naskah yang berkaitan dengan permasalahan yang dibahas. Data yang didapat kemudian dikumpulkan lalu disistematisasi.  Data yang sudah disistematisasi itu kemudian  dijelaskan untuk dapat menjawab permasalahan yang diajukan. Hasil yang didapat dari pembahasan dalam tulisan ini adalah: Indonesia berdasar KHL 1982 mempunyai kewenangan yang tegas dalam menindak pelaku illegal fishing, namun dalam pelaksanaan penanggulangan illegal fishing di wilayah ZEE masih mengalami beberapa hambatan.

  2. PERFORMA PRODUKSI DOMBA YANG DIBERI COMPLETE FEED FERMENTASI BERBASIS POD KAKAO SERTA NILAI NUTRIEN TERCERNANYA SECARA IN VIVO

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    Suci Wulandari

    2014-02-01

    Full Text Available Penelitian ini dimaksudkan untuk mengetahui nilai manfaat pod kakao sebagai bahan pakan complete feed ruminansia, khususnya domba. Tujuan penelitian ini adalah untuk mengevaluasi nutrien tercerna complete feed, berbahan baku utama pod kakao terfermentasi, dan mengetahui performa produksi domba. Perlakuan CF0 adalah complete feed tanpa fermentasi (kontrol, perlakuan CF1 adalah complete feed dengan penambahan pod kakao terfermentasi, dan perlakuan CF2 adalah complete feed fermentasi berbasis pod kakao. Replikasi masing-masing perlakuan sebanyak 6 ekor domba. Analisis statistik yang digunakan adalah analisis variansi pola searah dan hasil yang berbeda dilanjutkan dengan uji beda mean Duncan new Multiple Range Test. Hasil penelitian menunjukkan bahwa penambahan pod kakao terfermentasi pada complete feed maupun pakan complete feed fermentasi berbasis pod kakao tidak berpengaruh terhadap konsumsi bahan kering, protein, maupun bahan organik tercerna, serta tidak mempengaruhi pertambahan bobot badan harian dan konversi pakan. Nilai serat kasar tercerna secara in vivo terbaik terdapat pada pakan perlakuan CF2. (Kata kunci: Pod kakao, Fermentasi anaerob, Inokulum mikrobia, Complete feed, Domba

  3. BUDIDAYA RUMPUT LAUT, Kappaphycus alvarezii SECARA TERINTEGRASI DENGAN IKAN KERAPU DI TELUK GERUPUK KABUPATEN LOMBOK TENGAH, NUSA TENGGARA BARAT

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    I Nyoman Radiarta

    2014-03-01

    Full Text Available Budidaya ikan laut dalam keramba jaring apung menghasilkan banyak sisa pakan dan feses yang dapat meningkatkan kandungan nutrien berupa nitrogen dan fosfat perairan. Pemanfaatan nutrien tersebut dapat dilakukan melalui budidaya rumput laut di sekitar keramba ikan laut. Pengamatan pertumbuhan dan laju pertumbuhan spesifik terhadap dua varietas rumput laut (Kappaphycus alvarezii var. Maumere dan Tambalang telah dilakukan di Teluk Gerupuk, Lombok Tengah untuk satu siklus musim tanam pada bulan September-Oktober 2013. Hasil penelitian ini menunjukkan bahwa performansi pertumbuhan rumput laut yang terintegrasi dengan keramba ikan laut sangat baik. Laju pertumbuhan spesifik terbesar ditemukan pada varian Maumere yaitu berkisar antara 4,26%-4,68%/hari dibandingkan dengan varian Tambalang yaitu berkisar antara 3,90%-4,20%/hari. Secara umum melalui sistem budidaya multi-tropik terintegrasi (IMTA ini, peningkatan produksi rumput laut dapat mencapai 74% dibandingkan dengan sistem monokultur. Model IMTA sangat relevan dengan program ekonomi biru Kementerian Kelautan dan Perikanan dalam mendukung pengembangan perikanan budidaya yang berkelanjutan.

  4. UJI ZONA HAMBAT EKSTRAK DAUN PUTRI MALU (Mimosa pudica TERHADAP BAKTERI Staphylococcus aureus DAN Methicillin-resistant Staphylococcus aureus (MRSA SECARA IN VITRO

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    Nyoman Ririn Chandrika Sari

    2015-04-01

    Full Text Available Resistensi Staphylococcus aureus dan MRSA terhadap antibiotika spektrum luas mendorong berbagai penelitian untuk menemukan senyawa aktif yang sensitif dan efektif dalam menghambat pertumbuhan bakteri.Penelitian ini bertujuan untuk mengetahui efek penghambatan ekstrak daun putri malu (Mimosa pudica terhadap pertumbuhan bakteri Staphylococcus aureus dan MRSA.Penelitian terhadap aktivitas antimikrobial ekstrak daun putri malu dilakukan dengan metode agar difusi cakram dengan menggunakan metode Kirby-Bauer.Suspensi bakteri disesuaikan dengan standar kekeruhan Mc Farland 0.5. Biakan bakteri dalam cawan petri masing-masing diberikan 6 perlakuan, yaitu kontrol positif (amoxicillin atau vancomycin, kontrol negatif (alkohol, serta ekstrak daun putri malu dengan konsentrasi 25 mcg/ml, 50 mcg/ml, 75 mcg/ml dan 100 mcg/ml. Pertumbuhan Staphylococcus aureus dihambat secara signifikan oleh ekstrak daun putri malu, dengan efek inhibisi pada semua konsentrasi secara signifikan lebih baik dibandingkan dengan kontrol positif (p<0.05. Zona inhibisi Staphylococcus aureus pada konsentrasi 25 mcg/ml adalah 28.86 mm dan telah memenuhi kriteria sebagai antimikrobial sensitif berdasarkan Clinical and Laboratory Standard Institute (CLSI. Inhibisi pertumbuhan MRSA secara signifikan terjadi pada semua konsentrasi ekstrak daun putri malu dibandingkan dengan kontrol negatif (p=0.00. Zona inhibisi terbesar ditemukan pada konsentrasi 100 mcg/ml dengan diameter sebesar 14.16 mm dan memiliki efek antimikrobial sebanding dengan vancomycin dalam menghambat pertumbuhan MRSA (p=0.186. Hasil uji agar difusi cakram menunjukkan bahwa senyawa aktif dalam ekstrak daun putri malu memiliki aktivitas antimikrobial yang tinggi terhadap Staphylococcus aureus dan MRSA secara in vitro.

  5. PENGARUH PENGURANGAN RANSUM PAKAN SECARA PERIODIK TERHADAP PERTUMBUHAN, SINTASAN, DAN PRODUKSI UDANG VANAME (Litopenaeus vannamei POLA SEMI-INTENSIF DI TAMBAK

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    Abdul Mansyur

    2011-04-01

    Full Text Available Pakan merupakan biaya produksi tertinggi dalam budidaya udang vaname di tambak, sehingga diperlukan informasi strategi manajemen pakan dalam budidaya. Tujuan penelitian adalah mengetahui pengaruh pengurangan ransum pakan secara periodik terhadap pertumbuhan, sintasan, produksi, rasio konversi pakan dan efesiensi pakan pada budidaya udang vaname pola semi-intensif. Penelitian dilakukan di tambak percobaan Punaga Takalar, menggunakan 6 petak pembesaran udang vaname masingmasing berukuran 4.000 m2. Hewan uji adalah pasca larva udang vaname dengan bobot awal rata-rata 0,017 g yang ditebar pada tambak dengan kepadatan 20 ekor/m2. Rancangan penelitian adalah rancangan acak lengkap dengan tiga perlakuan yang masing-masing perlakuan terdiri atas dua ulangan. Perlakuan yang diujicobakan adalah pengurangan ransum pakan (pemuasaan secara periodik yaitu: A pengurangan ransum pakan 30%, B pengurangan ransum pakan 60%, dan C kontrol (tanpa pengurangan ransum pakan. Hasil penelitian menunjukkan bahwa pengurangan ransum pakan secara periodik berpengaruh tidak nyata (P>0,05 terhadap pertambahan bobot mutlak, laju pertumbuhan harian, sintasan, produksi, dan rasio konversi pakan bahkan mampu meningkatkan efisiensi pakan sekitar 7,71–22,39%. Penghematan penggunaan pakan untuk udang vaname dapat dilakukan dengan pengurangan ransum pakan hingga 60% bobot badan/hari/minggu

  6. BUDIDAYA TABAT BARITO (Ficus deltoidea JACK SECARA STUMP DENGAN VARIASI PERLAKUAN MEDIA TANAM DAN PUPUK ORGANIK NASA

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    Yudi Firmanul Arifin

    2016-09-01

    Full Text Available ABSTRACT.  Indonesia is rich of medicinal plants for some ecosystem types of forest, one of which is tabat barito (Ficus deltoidea Jack. The utilization of tabat barito mainly used for women's health and other using. Until present day its use continues to increase, especially for traditional medicines, but efforts for cultivation have not been much done. To overcome the sustainability of raw material, the research of cultivation systems have to be done. The vegetative methods which are using stump is an alternative for reproduction.  The treatments of media and liquid fertilizer NASA were given of every treatment for improving survival rate and the increment of height and diameter.  Result of research showed the treatment for media of top soil was better than sand for height and number of leaf parameter.  The treatment of organic fertilizer was only improving number of leaves.  Keywords: vegetative,  stump,  organic fertilizer, growth ABSTRAK.  Indonesia sangat kaya akan tumbuhan berkhasiat obat yang tumbuh pada berbagai tipe ekosistem hutan.  Tabat barito (Ficus deltoidea Jack adalah salah satu tumbuhan yang sudah dikenal oleh masyarakat berkhasiat obat untuk berbagai penyakit, khususnya untuk kesehatan wanita setelah melahirkan dan penggunanya pun untuk bahan baku industri obat tradisional sudah dilakukan hingga kini.  Untuk mengatasi kekhawatiran akan keberlanjutan bahan baku tersebut, maka penelitian budidaya tumbuhan ini dilakukan.  Metode yang digunakan adalah secara vegetatif dengan cabutan anakan alam (stump.  Perlakuan terhadap media tanam dan pemberian pupuk cair NASA diberikan pada setiap metode untuk meningkatkan daya hidup dan mempercepat pertumbuhan anakan.  Hasil yang diperoleh menunjukkan bahwa Perlakuan media top soil lebih baik dibandingkan dengan media pasir pada parameter tinggi dan jumlah daun.  Perlakuan pemberian pupuk organik cair NASA hanya dapat meningkatkan pertambahan jumlah daun. Kata kunci: vegetatif

  7. 临床护理工作中实施连续排班模式的效果观察%Practice of APN Scheduling Model and Its Effect Evaluation

    Institute of Scientific and Technical Information of China (English)

    刘冰; 张娟; 刘花林

    2011-01-01

    目的:探讨尝试连续排班模式(APN排班)改革护理排班方式,全面提高临床护理质量,实现优质护理服务.方法:改革原有传统护理排班方式,通过设立APN班,制定各班次的工作流程与质量标准,应用于临床护理工作.比较实施前后患者服务满意率、护理质量、护理职业满意率.结果:通过实施该项改革尝试,患者对护理工作满意率,病人基础护理合格率、病房管理合格率有显著性差异(P<0.05).结论:APN连续排班模式有助干科学管理人力资源,为患者提供安全、高效的护理服务,满足患者、护士的需求话应新的医学模式需求.%Objective: to discuss raising the quality of clinical care through reforming the APN schedule model of nursing. Methods: To reform the traditional style of arrangement of nursing schedules, new rules that establishing APN duty,comparsion of patient before and after the service satisfaction quality of care nursing job satisfaction. Result:Through this reforming, the degree of the patient satisfaction, the rate of qualification of basic nursing and the rate of qualification of ward administration were significantly different compliance rates (p<0. 05). Conclusion- APN schedule model can improve rationl usage and allocation of nursing human resources, demands the needs of pations and nurse, adapted to the new medical modle needs.

  8. APN arranges in groups with nurse the level management in the critically ill guardianship hospital ward application%APN排班与护士层级管理在重症监护病房中的应用

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    严艳玲; 李云玲; 梁冬梅; 罗进玲; 陈清

    2010-01-01

    目的 探讨APN排班与护士的层级管理在重症监护专科护理管理中的应用效果.方法 改变传统的排班模式,实施APN连续性分组排班,每小组均实行护理组长-责任护士-助理护士层级管理,新老搭配,使各项护理工作均能在监督指导下落实.结果 APN排班各班的护理人力安排均衡且交接次数比传统排班明显减少.实施APN排班与护士层级管理后,危重患者护理合格率由90.50%提高到98.50%,差异有显著性(P<0.05).结论 ICU开展APN连续排班和层级管理,有利于合理安排人力,分组排班,充分发挥团队协作精神及各层级护理人员的作用,有效降低年轻护士在岗时因专业能力不足所带来的安全隐患,确保护理安全,促进专科发展,全面提高护理质量.%Objective To explore the APN scheduling's and the hierarchical nurse management's effect on special nursing management of ICU.Methods Change the traditional Scheduling,and carry out the APN scheduling and the hierarchical nurse management by the experienced nurses and the less-experienced ones. All the nursing job is completed under supervising. Results After the practice of APN Scheduling, the nurse management is more rational. And the qualified rate of ICU nursing got a raise from 90.50% to 98.50%. Conclusions Carrying out the APN scheduling and the hierarchical nurse management in the ICU, can make the human capital arrangement more rational, and get a better team work. What's more, it can promote the development of the special nursing and the nursing quality.

  9. PENGENDALIAN PENYAKIT PATIK (Cercospora nicotianae PADA TEMBAKAU NA OOGST SECARA IN-VIVO DENGAN EKSTRAK DAUN GULMA KIPAHIT (Tithonia diversifolia

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    Aditya Reza Apriyadi

    2013-11-01

    daun kipahit; Cercospora nicotianae; Tembakau How to citate: Apriyadi AR, WS Wahyuni, V Supartini. 2013. Pengendalian penyakit patik (Cercospora nicotianae pada tembakau na oogst secara in-vivo dengan ekstrak daun gulma kipahit (Tithonia diversifolia. Berkala Ilmiah Pertanian 1(2: 30-32.

  10. Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus aurantifolia s. Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro

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    Abdul Razak

    2013-01-01

    Full Text Available AbstrakJeruk Nipis (Citrus aurantifolia S. merupakan salah satu tanaman obat keluarga yang banyak terdapat ditengah masyarkat dan banyak digunakan sebagai ramuan tradisional. Bagian yang sering digunakan adalah air perasannya, dengan salah satu manfaat dapat digunakan untuk menghilangkan jerawat serta penyembuhan luka agar tidak terjadi abses. Jerawat dan abses pada luka merupakan salah satu infeksi yang disebabkan oleh bakteri Staphylococcus aureus.Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S. terhadap pertumbuhan bakteri Staphylococcus aureus secara invitro. Penelitian dilakukan dengan metoda eksperimental laboratorium dengan desain postest only control group design yang dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas.Hasil penelitian menunjukan bahwa air perasan buah jeruk nipis memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan berbagai konsentrasi yaitu 25%, 50%, 75%, dan 100% dan terdapat pengaruh lama kontak terhadap pertumbuhan bakteri dimana bakteri tidak tumbuh seteleh kontak 5 menit pertama dan diikuti menit-menit berikutnya dengan air perasan buah jeruk nipis konsentrasi 100%. Jadi, semakin tinggi konsentrasi air perasan buah jeruk nipis dan semakin lama kontak dengan bakteri Staphylococcus aureus maka daya hambatnya semakin baik.Kata kunci: Uji Daya Hambat, Air Perasan Buah Jeruk Nipis, Staphylococcus aureus.Abstract Lime (Citrus aurantifolia S. is kind of family’s herbal medicine, most using in the community is widely used as a traditional herb. The most common used part is the lime fruit squeeze with one of the function is used for removing acne and wound healing to prevent the form of abscess. Pimples and abscesses of the wound is one of the infections caused by the bacterium Staphylococcus aureus.The purpose of this study was to determine the inhibition of lime fruit (Citrus aurantifolia S. squeeze

  11. Perbandingan Daya Hambat Madu Alami dengan Madu Kemasan secara In Vitro terhadap Streptococcus beta hemoliticus Group A sebagai Penyebab Faringitis

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    Elsi Wineri

    2014-09-01

    Full Text Available AbstrakMadu merupakan substansi alam yang dihasilkan oleh lebah yang diketahui memiliki manfaat, salah satunya untuk mengobati faringitis yang disebabkan Streptococcus beta hemoliticus Group A. Efek antibakteri dari madu dapat menghambat pertumbuhan  Streptococcus beta hemoliticus Group A. Berdasarkan cara pembuatannya madu terdiri dari madu alami dan madu kemasan. Penelitian ini bertujuan untuk mengetahui perbedaan daya hambat madu alami dengan madu kemasan secara in vitro terhadap Streptococcus beta hemoliticus Group A. sebagai penyebab faringitis. Jenis penelitian ini adalah eksperimental dengan rancangan posttest only control group design yang dilaksanakan dari September sampai Desember 2013 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Hasil penelitian menunjukan madu alami dan madu kemasan dapat menghambat pertumbuhan Streptococcus beta hemoliticus Group A dengan diameter daya hambat terbesar pada madu alami adalah 14 mm dan madu kemasan 11 mm. Berdasarkan uji analisis Kruskal-Wallis yang dilanjutkan dengan post-hoc Mann-Whitney terdapat perbedaan yang signifikan antara daya hambat  madu alami dengan madu kemasan dengan nilaip=0,004 (p<0,05. Kesimpulan hasil penelitian adalah madu alami dan madu kemasan memiliki daya hambat terhadap pertumbuhan Streptococcus beta hemoliticus Group A. Madu alami memiliki daya hambat yang lebih kuat dibandingkan madu kemasan.Kata kunci: madu alami, madu kemasan, Streptococcus beta hemoliticus Group A, antibakteri, faringitis AbstractHoney is a natural substance that produced by bees which is known have many benefits, one of them is to treat pharyngitis that caused by Streptococcus beta hemoliticus Group A. The antibacterial effect of honey can inhibit bacterial growth. By way of making, honey is divided to natural honey dan packing honey. The purpose of this study was to see comparison of the antibacterial effect of natural honey and packing honey againt Streptococcus beta

  12. Research on Construction Project Post-evaluation Based on APN%基于APN工程建设项目后评价研究

    Institute of Scientific and Technical Information of China (English)

    高运孝; 李熹

    2015-01-01

    工程建设项目后评不仅能够对工程建设项目在竣工后能否达到预期的效用进行验证,而且还能通过总结上一期工程建设经验,为下一期工程项目建设实施奠定,近年来,备受各政府主管部门和项目实施单位的高度重视。本文基于网络分析法(APN),从工程项目建设实施过程、获得的效益、影响项目后期效果的因素及影响项目可持续性的因素等四个维度,建立后评价的模型,对工程项目后进行,确定各子工程项目对项目后效果的优势度及风险因素,以期提高工程建设项目后评价的效用。%Construction project post-evaluation can not only test whether the construction project has achieved the expected effect, but also provide basis for the next construction phase through summarizing experience from the project. So it is valued by the related departments of the government and the project implementing units. Based on APN method, this article builds a post-evaluation model from four dimensions: project implementing process, benefit, factors affecting the effect of the project and factors affecting the project sustainability to do post evaluation to the project and decide the dominance degree and risk factor of each sub-project to the project post effect so as to improve the effectiveness of construction project post-evaluation.

  13. Anestesia e apnéia obstrutiva do sono Anestesia y apnea obstructiva del sueño Anesthesia and obstructive sleep apnea

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    Charles Machado

    2006-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A manutenção da permeabilidade das vias aéreas superiores (VAS é fundamental para anestesia e para pacientes com apnéia obstrutiva do sono (AOS. Durante ambos os estados ocorre uma redução do tônus da musculatura faríngea. Identificar pacientes com AOS é importante a fim de prevenir riscos durante o período perioperatório. O objetivo deste trabalho foi apresentar uma revisão sobre a relação entre AOS e anestesia, levando em conta o planejamento da anestesia, enfatizando a importância da identificação da síndrome da apnéia e hipopnéia obstrutiva do sono (SAHOS. CONTEÚDO: A SAHOS ocorre principalmente por colapso total ou parcial da faringe, podendo levar a diminuição na saturação da oxiemoglobina e complicações cardiovasculares. Os principais fatores predisponentes são sexo masculino, obesidade, características crânio e orofaciais. Seu diagnóstico é clínico e polissonográfico, o que também quantifica a gravidade da AOS. Os pacientes com SAHOS especialmente acentuada podem apresentar problemas durante a intubação traqueal e sedação, estando alguns mais susceptíveis à ocorrência de hipóxia e hipercapnia, mesmo na vigência de pulmões normais. Os autores discutem a importância do diagnóstico prévio e tratamento da SAHOS na tentativa de reduzir o risco anestésico. CONCLUSÕES: O diagnóstico e tratamento prévio da SAHOS com pressão positiva contínua nas VAS podem reduzir complicações perioperatórias e influenciar na conduta anestésica e na recuperação pós-anestésica.JUSTIFICATIVA Y OBJETIVOS: El mantenimiento de la permeabilidad de las vías aéreas superiores (VAS es fundamental para la anestesia y para pacientes con apnea obstructiva del sueño (AOS. Durante los de los estados, ocurre una reducción del tono de la musculatura faríngea. Identificar pacientes con AOS es importante para prevenir riesgos durante el período perioperatorio. El objetivo de este

  14. Karakterisasi Unjuk Kerja Diesel Engine Generator Set Sistem Dual Fuel Solar-Syngas Hasil Gasifikasi Briket Municipal Solid Waste (MSW Secara Langsung

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    Achmad Rizkal

    2017-01-01

    Full Text Available Sejalan dengan semakin banyaknya kebutuhan energi untuk dapat digunakan sebagai bahan bakar maka perlu adanya pengembangan gas biomassa sebagai bahan bakar alternatif pada motor pembakaran dalam maka akan dilakukan penelitian mengenai aplikasi sistem dual fuel gas hasil gasifikasi biomassa municipal solid waste (msw pada sistem downdraft dengan minyak solar pada motor diesel stasioner. Penelitian ini bertujuan untuk mengetahui seberapa besar solar yang tersibtitusi dengan adanya penambahan syngas yang disalurkan secara langsung. Penelitian ini dilakukan secara eksperimental dengan proses pemasukan aliran syngas yang dihasilkan downdraft municipal solid waste (MSW kedalam saluran udara mesin diesel generator set secara langsung menggunakan sistem mixer. Pengujian dilakukan dengan putaran konstan 2000 rpm dengan pembebanan bervariasi dari 200 watt sampai dengan 2000 watt dengan interval 200 watt. Bahwa produksi syngas dari reaktor gasifikasi ditambahkan sistem bypass untuk mengetahui kesesuaian antara reaktor gasifikasi dan mesin generatorset data ṁ syngas yang dibutuhkan mesin diesel, ṁ syngas yang di bypass untuk mendapatkan kesesuaian antara produksi syngas dan yang di bypass.  Data-data yang diukur dari penelitian ini menunjukkan bahwa besar nilai mass flowrate gas syngas yang dibutuhkan mesin diesel pada AFR reaktor gasifier 1,39 sebesar 0,0003748 kg/s. Mass flowrate gas syngas yang di bypass menunjukkan nilai 0 pada saat sistem dijalankan karena seluruh gas syngas masuk kedalam ruang bakar. AFR rata-rata sebesar 14,54 ,Nilai Spesifik fuel consumption (sfc mengalami peningkatan 68% dari kondisi standar single fuel , Nilai efesiensi thermal mengalami kenaikan sebesar 7% dari kondisi single fuel, Nilai daya rata-rata sebesar 2,28kW, Nilai torsi rata-rata sebesar 10,94 N.m. Solar yang tersibtitusi sebesar 48%. Nilai temperatur (coolant, mesin, oil, dan gas buang pada setiap pembebanan mengalami kenaikan.

  15. UJI AKTIVITAS ANTIBAKTERI PADA KOMBINASI EKSTRAK ETANOL KURKUMIN (Curcumin longa L DAN MADU LOKAL TERHADAP BAKTERI METHICILLIN-SENSITIVE STAPHYLOCOCCUS AUREUS SECARA IN VITRO

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    Marco Reeiner

    2015-06-01

    Full Text Available Penggunaan obat tradisional dapat menjadi terobosan sebagai pengobatan alternatif seiring dengan meningkatnya resistensi terhadap antibiotik. Kurkumin (Curcumin longa L telah dilaporkan mengandung zat terapi, dikarenakan memiliki kapasitas sebagai obat. Penelitian ini bertujuan untuk lebih menjelaskan kegiatan antibakteri ekstrak kurkumin dan madu lokal. Penelitian eksperimental secara in vitro ini dilakukan dengan membuat ekstrak kurkumin dengan cara maserasi dan dilanjutkan dengan uji resistensi bakteri Staphylococcus aureus. Kegiatan uji antibakteri yang telah dilakukan dibandingkan dengan kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak kurkumin dan madu lokal tidak memiliki daya bunuh dan daya hambat terhadap bakteri Staphylococcus aureus.

  16. Pengaruh Aplikasi Bahan Pemutih Gigi Karbamid Peroksida 10% dan Hidrogen Peroksida 6% secara Home Bleaching terhadap Kekerasan Permukaan Email Gigi

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    Meiyestri Dwi Riani

    2015-05-01

    Full Text Available AbstrakHome bleaching adalah teknik pemutihan gigi vital yang dilakukan oleh pasien di rumah dalam pengawasan dokter gigi dengan konsentrasi karbamid peroksida 10-16% atau 3-6% hidrogen peroksida. Tujuan penelitian ini adalah untuk mengetahui pengaruh aplikasi bahan pemutih gigi karbamid peroksida 10% dan hidrogen peroksida 6% secara home bleaching terhadap kekerasan permukaan email gigi. Metode penelitian adalah true experimental dengan pretest-posttest control group design. Sampel berupa gigi premolar satu rahang atas yang sudah diekstraksi sebanyak 32 buah yang dibagi menjadi dua kelompok. Kelompok 1 adalah kelompok aplikasi bahan pemutih gigi karbamid peroksida 10% dengan lama aplikasi 1 jam perhari selama 14 hari dan kelompok II adalah kelompok aplikasi bahan pemutih gigi hidrogen peroksida 6% dengan lama aplikasi 10 menit perhari selama 14 hari. Selama aplikasi kedua sampel berada dalam inkubator 370C. Kekerasan permukaan email gigi diukur dengan alat Vickers Harndness Tester. Analisis data yang digunakan adalah uji t independen dan uji t dependen (p<0,05. Hasil uji statistik menunjukkan terjadinya penurunan yang sangat signifikan 0,000 (p<0,05 pada rerata nilai kekerasan permukaan email gigi antara sebelum dan setelah aplikasi bahan pemutih gigi. Kedua bahan pemutih gigi ini mempunyai perbandingan yang cenderung tidak signifikan 0,073 (p>0,05 terhadap kekerasan permukaan email gigi setelah aplikasi bahan pemutih gigi. Disimpulkan bahwa bahan pemutih gigi dapat menyebabkan penurunan kekerasan permukaan email gigi yang sangat signifikan karena terjadinya demineralisasi.Kata kunci: karbamid peroksida, hydrogen peroksida, home bleaching, kekerasan permukaan email gigiAbstractHome bleaching is whitening treatment of vital teeth conducted by a patient at home with under control of a dentist. The consentration of bleaching agents are 10-16% of carbamid peroxide or 3-6% of hidrogen peroxide. The objective of this study was to investigate the

  17. PEMBENTUKAN TUNAS DARI BIJI MANGGIS (Garcinia mangostana L. ASAL BENGKALIS DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO

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    Mayta Novalida Isda

    2016-12-01

    Full Text Available AbstrakGarcinia mangostana L. merupakan salah satu spesies dari famili Guttiferae kaya senyawa dari golongan xanthon. Manggis memiliki juvenil yang lama. Salah satu metode yang dapat memecahkan masalah dalam perbanyakan tanaman manggis dalam jumlah yang banyak, bisa sepanjang musim dan menghasilkan tanaman yang seragam yaitu kultur in vitro. Tujuan dari penelitian ini adalah untuk menentukan konsentrasi terbaik antara Benzil Amino Purin (BAP dan madu terhadap induksi tunas dari eksplan biji manggis yang belah tiga melintang  secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL dengan perlakuan BAP (0;3 mg/L dan madu (3;6 mg/L pada media MS (Murashige-Skoog dengan pengamatan 70 hari setelah tanam (hst. Hasil penelitian menunjukkan bahwa pemberian BAP maupun madu mampu meningkatkan pembentukan tunas. Pemberian BAP dan madu berpengaruh nyata terhadap persentase terbentuknya tunas, waktu muncul tunas, jumlah tunas dan panjang tunas.  Jumlah tunas terbanyak terdapat pada perlakuan 3 mg/L BAP sebanyak 20 tunas per biji. Kombinasi perlakuan dengan penambahan 3 mg/L BAP + 3 ml/L madu menghasilkan persentase terbentuk tunas tertinggi (100%; waktu muncul tunas tercepat 12,75 hst; dan panjang tunas tertinggi 1,86 cm. Pada penelitian ini telah berhasil menginduksi tunas dari biji manggis yang dibelah tiga melintang asal Bengkalis dengan penambahan BAP dan madu.AbstractGarcinia mangostana L. is one of the species within the family Guttiferae that rich in compounds from the class of xanthon. Mangosteen has time juvenile problems. One method that can solve the problem in the mangosteen plant propagation which can be produced in a large number in all season and uniform is in vitro culture. The purpose of this study was to determine the best concentration of BAP and honey to induce the formation of shoot from explants of mangosteen seeds split transversely into three pieces by in vitro method. This study used a Completely Randomized Design

  18. Perbandingan Efek Antibakteri Madu Asli Sikabu dengan Madu Lubuk Minturun terhadap Escherichia Coli dan Staphylococcus Aureus secara In Vitro

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    Yugo Berri Putra Rio

    2012-09-01

    Full Text Available AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability

  19. Sleep apnea and REM sleep behavior disorder in patients with Chiari malformations Apnéia do sono e distúrbio do comportamento da fase do sono com REM em pacientes com malformações de Chiari

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    Paulo Sérgio A. Henriques-Filho

    2008-06-01

    Full Text Available BACKGROUND: Chiari malformations (CM may result in the appearance of REM sleep behavior disorder (RBD and sleep apnea syndrome (SAS that can be considered markers of brain stem dysfunction. PURPOSE: To evaluate the frequency of RBD and SAS in patients with CM type I and II. METHOD: Were evaluated 103 patients with CM by means of full night polysomnography. Were scoring different sleep stages, frequency of abnormal movements (through video monitoring and abnormal respiratory events. RESULTS: Of the 103 patients, 36 showed CM type I and 67 CM type II. Episodes of RBD were observed in 23 patients. Abnormal apnea-hypopnea index (AHI was observed in 65 patients. CONCLUSION: The high rate of RBD suggests that this parassomnia and the increased frequency of central sleep apnea episodes, may be considered as a marker of progressive brain stem dysfunction.INTRODUÇÃO: Malformações de Chiari (MC podem gerar o aparecimento de distúrbio comportamental da fase do sono com REM (DCR e síndrome da apnéia do sono (SAS, sugerindo a ocorrência de disfunção do tronco cerebral. OBJETIVO: Avaliar a freqüência de DCR e SAS em pacientes com MC I ou II. MÉTODO: Utilizou-se a polissonografia de noite inteira para a avaliação de 103 pacientes. Classificaram-se as diferentes fases do sono e analisou-se a freqüência de movimentos anormais (monitorada por vídeo e de eventos respiratórios anormais. RESULTADOS: Dos 103 pacientes analisados, 36 eram portadores de MC I e 67 de MC II. Episódios de DCR foram observados em 23 pacientes. O índice de apnéia/hipopnéia foi considerado anormal em 65 pacientes. CONCLUSÃO: A alta freqüência de DCR e o aumento da freqüência de episódios de apnéia central do sono podem ser considerados manifestação de disfunção progressiva do tronco cerebral.

  20. PERBANDINGAN ANALISIS KROMATOGRAFI CAIR KINERJA TINGGI ELUASI GRADIEN DENGAN ISOKRATIK PADA PENENTUAN VITAMIN B1, B2 DAN B6 DALAM SEDIAAN SIRUP MULTIVITAMIN SECARA SIMULTAN

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    Ni Luh Kasih Ariani

    2015-06-01

    Full Text Available ABSTRAK: Vitamin B terutama B1 (Tiamin, B2 (Riboflavin, dan B6 (Piridoksin sering terkandung dalam sirup multivitamin sehingga diperlukan analisis untuk mendeteksi secara simultan dalam campuran. Tujuan penelitian ini adalah membandingkan analisis vitamin B1, B2, dan B6 dalam sirup multivitamin secara simultan dengan kromatografi cair kinerja tinggi eluasi gradien dan isokratik, menggunakan kolom C18, panjang 15 cm, dan dengan pelarut campuran metanol: air : asam asetat glasial (10:90:1. Fase gerak adalah campuran natrium heksan sulfonat 5 mM dalam asam asetat glasial 0,5% dengan metanol yang dicampur secara gradien. Kondisi optimum metode gradien diperoleh pada laju alir 2,0 mL/menit, panjang gelombang 280 nm dengan waktu retensi 3,441 menit untuk piridoksin; 4,985 menit untuk riboflavin dan 7,393 menit untuk tiamin dengan resolusi 2,272 antara riboflavin dan piridoksin. Hasil uji presisi riboflavin dan piridoksin menggunakan metode isokratik (campuran natrium heksan sulfonat 5 mM dengan metanol dengan perbandingan 70 : 30 masing-masing dengan RSD adalah 1,377 dan 1,376 sedangkan metode gradien adalah 0,693 dan 0,825. Uji linearitas ketiga vitamin menggunakan dua metode isokratik dan gradien memenuhi persyaratan dengan R2 = 0,999. Kata kunci : Vitamin B, sirup multivitamin, kromatografi cair kinerja tinggi ABSTRACT: The aim of this research is to compare the simultaneous analytical results of B1 (Tiamin, B2 (Riboflavin, and B6 (Piridoxin in multivitamin syrup between gradient and isocratic methods using high performance liquid chromatography (HPLC. The separation was performed on 15 cm length of C18 column and a mixture of methanol, water and glacial acetate acid with ratio of 10:90:1 was used as solvent. The eluent was a mixture of methanol and 5 mM sodium hexane sulfonate in 0.5% glacial acetate acid and gradually mixed using 2 different pumps. The optimal analytical conditions for gradient method were found to be 2,0 mL/min of flow rate and

  1. Sintomas da síndrome de apnéia-hipopnéia obstrutiva do sono em crianças Symptoms of obstructive sleep apnea-hypopnea syndrome in children

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    Paloma Baiardi Gregório

    2008-06-01

    Full Text Available OBJETIVOS: Investigar os sintomas mais freqüentes encontrados em crianças com diagnóstico polissonográfico de síndrome da apnéia-hipopnéia obstrutiva do sono (SAHOS. MÉTODOS: Foram avaliadas 38 crianças consecutivamente encaminhadas ao laboratório do sono com suspeita de SAHOS no período de junho de 2003 a dezembro de 2004. Os pacientes foram submetidos a um questionário pré-sono e a polissonografia. RESULTADOS: A idade média foi de 7,8 ± 4 anos (variação, 2-15 anos, sendo 50% das crianças do sexo masculino. Não apnéicos corresponderam a 7,9% dos pesquisados, distúrbio leve obstrutivo do sono ocorreu em 42,1%, moderado em 28,9% e severo em 22,1%. Observou-se maior freqüência de casos severos de apnéia entre crianças menores de seis anos (idade pré-escolar. Dentre as crianças com SAHOS, os sintomas mais citados foram ronco e obstrução nasal, presentes em 74,3 e 72,7% das crianças, respectivamente. Sonolência excessiva e bruxismo ocorreram em, respectivamente, 29,4 e 34,3% dos casos e doença do refluxo em apenas 3,1%. Agitação das pernas e dificuldade para iniciar o sono foram encontradas em, respectivamente, 65 e 33% dos avaliados. Todas as crianças que apresentaram SAHOS de grau severo tinham queixa de ronco e bruxismo. CONCLUSÕES: Nossos resultados mostraram que os sintomas mais freqüentes em crianças e adolescentes com SAHOS são ronco e obstrução nasal. Além disso, quadros mais graves da SAHOS estão associados à menor faixa etária.OBJECTIVE: To investigate the symptoms most frequently found in children with a polysomnographic diagnosis of obstructive sleep apnea-hypopnea syndrome (OSAHS. METHODS: We evaluated 38 children consecutively referred to the sleep laboratory with suspicion of OSAHS between June of 2003 and December of 2004. The patients were submitted to a pre-sleep questionnaire and to polysomnography. RESULTS: The mean age was 7.8 ± 4 years (range, 2-15 years, and 50% of the children

  2. Atualização em síndrome da apnéia obstrutiva do sono na infância Update in obstructive sleep apnea syndrome in children

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    Aracy P. S. Balbani

    2005-02-01

    Full Text Available A prevalência de SAOS em crianças é de 0,7-3%, com pico de incidência nos pré-escolares. Fatores anatômicos (obstrução nasal severa, más-formações craniofaciais, hipertrofia do tecido linfático da faringe, anomalias laríngeas, etc. e funcionais (doenças neuromusculares predispõem à SAOS na infância. A principal causa da SAOS em crianças é a hipertrofia adenotonsilar. As manifestações clínicas mais comuns são: ronco noturno, pausas respiratórias, sono agitado e respiração bucal. A oximetria de pulso noturna, a gravação em áudio ou vídeo dos ruídos respiratórios noturnos e a polissonografia breve diurna são métodos úteis para triagem dos casos suspeitos de SAOS em crianças, e o padrão-ouro para diagnóstico é a polissonografia em laboratório de sono durante uma noite inteira. Ao contrário dos adultos com SAOS, as crianças costumam apresentar: menos despertares associados aos eventos de apnéia, maior número de apnéias/hipopnéias durante o sono REM e dessaturação mais acentuada da oxihemoglobina mesmo nas apnéias de curta duração. O tratamento da SAOS pode ser cirúrgico (adenotonsilectomia, correção de anomalias craniofaciais, traqueostomia ou clínico (higiene do sono, pressão positiva contínua nas vias aéreas - CPAP.The prevalence of OSAS in children is 0.7-3%, with peak incidence in pre-schoolers. It is characterised by partial or complete upper airway obstruction during sleep, causing intermittent hypoxia. Both anatomical (severe nasal obstruction, craniofacial anomalies, hypertrophy of the pharyngeal lymphoid tissue, laryngeal anomalies, etc. and functional factors (neuromuscular diseases predispose to OSAS during childhood. The main cause of OSAS in children in adenotonsillar hypertrophy. The most common clinical manifestations of OSAS are: nocturnal snoring, respiratory pauses, restless sleep and mouth breathing. Nocturnal pulse oximetry, nocturnal noise audio/videotape recording and nap

  3. Neurocristopatia no diagnóstico diferencial das apnéias do recém nascido: relato de caso Neurochristopathy in the differential diagnosis of newborn's apnea: case report

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    Magda Lahorgue Nunes

    2001-12-01

    Full Text Available OBJETIVO: incluir a neurocristopatia na investigação etiológica das apnéias refratárias do recém nascido e discutir o valor diagnóstico da polissonografia. MÉTODO: relato de caso e discussão crítica da literatura. RESULTADOS: relatamos o caso de um recém-nascido que apresentou disfunção ventilatória nas primeiras horas de vida associada a distensão abdominal, necessitando de ventilação mecânica contínua com piora do quadro respiratório durante o sono. Após o diagnóstico polissonográfico de hipoventilação foi investigado com exames de neuroimagem e biópsia de cólon, positiva para doença de Hirschprung. CONCLUSÃO: a neurocristopatia é uma síndrome neonatal que por vezes pode ter o seu reconhecimento dificultado pelo amplo espectro clínico associado. A polissonografia foi fundamental nesta investigação confirmando a hipoventilação. Este diagnóstico etiológico deve ser considerado na investigação de recém-nascidos com apnéias persistentes durante o sono.OBJECTIVE: to include neurocristopathy on the etiological workup of neonatal apneas and discuss the importance of polysomnography in this diagnosis. METHOD: case report and critical review of the literature. Results: we report on a newborn that presented respiratory failure in the first hours of life associated to abdominal distention. Continuous ventilatory support was necessary, and the respiratory distress increased during sleep. After polysomnographic confirmation of hypoventilation the newborn was submitted to neuroradiolgic tests and colon byopsy, positive to Hirschsprung's disease. CONCLUSION: Neurocristopathy syndrome can have many different clinical expression, and sometimes the syndrome can be misdiagnosed. Polysomnography confirms central hypoventilation. This diagnosis should be considered in the newborn's persistent apnea workup.

  4. Studi Numerik Pengaruh Convergency Promoters (CPs Terhadap Karakteristik Aliran Dan Perpindahan Panas Dengan ℓ/D = 0.25, Pada Tube Banks Yang Tersusun Secara Staggered

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    Chairunnisa Chairunnisa

    2013-09-01

    Full Text Available Compact heat exchanger merupakan jenis alat penukar kalor yang banyak digunakan didunia industri gas, refrigerasi dan tata udara. Dalam hal performa, compact heat exchanger bergantung pada pola permukaan fin, yakni wavy dan straight fin. Straight fin, struktur permukaan fin yang datar membuat aliran membutuhkan waktu yang relatif lebih lama untuk terjadi perpindahan panas dibandingkan tipe wavy fin yang bergelombang. Selain merubah pola permukaan, upaya untuk memaksimalkan perpindahan panas pada straight fin juga dapat dibentuk dengan penambahan Convergency Promoters (CPs pada permukaannya. Penelitian ini dilakukan dengan metode simulasi numerik dengan software Fluent 6.3.26. Simulasi ini dikondisikan dengan menggunakan model turbulensi k-ε RNG dan metode second-order upwind scheme. Pada penelitian ini yang divariasikan adalah Reynolds number berbasis diameter tube, yaitu 3000, 4000 dan 5000, dengan ukuran CPs, ℓ/D = 0,25, pada tube banks yang tersusun secara staggered . Fluida kerja yang digunakan adalah udara yang dimodelkan sebagai gas ideal yang mengalir melintasi celah antar tube dengan temperatur inlet 347.14 K dan temperatur tube (air konstan sebesar 310.5 K. Dari hasil simulasi ini didapatkan visualisasi kontur kecepatan, temperatur dan visualisasi pola aliran yang terbentuk serta pembuktian hipotesa bahwa dengan adanya penambahan CPs akan meningkatkan perpindahan panas. Dimana, model modified akan meningkatkan nilai Nusselt number dan koefisien konveksi sebesar 47- 63% daripada model baseline (tanpa penambahan CPs.

  5. Ronco habitual e apnéia obstrutiva observada em adultos: estudo de base populacional, Pelotas, RS Habitual snoring and obstructive sleep apnea in adults: population-based study in Southern Brazil

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    Ricardo B Noal

    2008-04-01

    Full Text Available OBJETIVO: Estimar a prevalência de ronco habitual e apnéia obstrutiva observada e fatores associados. MÉTODOS: Estudo transversal com amostra representativa de 3.136 adultos, com 20 anos ou mais, residentes em Pelotas, Rio Grande do Sul, em 2005. Informações sobre os desfechos e variáveis demográficas, socioeconômicas, comportamentais e antropométricas foram coletadas por meio de questionário. A análise estatística bruta e ajustada foi realizada utilizando-se teste exato de Fisher ou qui-quadrado de tendência linear e regressão de Poisson, respectivamente. RESULTADOS: A prevalência encontrada de ronco habitual foi de 50,5% (IC 95%: 48,1;52,8 e de apnéia obstrutiva de 9,9% (IC 95%: 8,7;11,2. Na análise ajustada, o relato de ronco foi maior nos homens (Razão de Prevalências - RP=1,25; IC 95%: 1,16;1,34, nos idosos (RP=1,62; IC 95%: 1,46;1,80, nos tabagistas (RP=1,15; IC 95%: 1,07;1,25, nos alcoolistas (RP=1,17; IC 95%: 1,03;1,31 e nos obesos (RP 1,71, IC95% 1,55;1,88. O relato de apnéia obstrutiva foi maior nos homens (RP=2,05; IC 95%: 1,67;2,52, nos idosos (RP=2,23; IC 95%: 1,64;3,03, nos tabagistas (RP=1,60; IC 95%: 1,25;2,05 e nos obesos (RP=2,61; IC 95%: 1,97;3,47. CONCLUSÕES: Ronco habitual e apnéia obstrutiva foram sintomas comuns na população estudada. Fatores de risco conhecidos como sexo masculino e idade entre quarta e quinta décadas de vida não são modificáveis. Entretanto, tabagismo, alcoolismo e obesidade também associados aos desfechos, devem ser identificados e tratados na população geral.OBJECTIVE: To estimate the prevalence of habitual snoring and obstructive sleep apnea and their factors associated. METHODS: Cross-sectional study with a representative sample comprising 3,136 adults (>20 years living in the city of Pelotas, Southern Brazil, in 2005. A questionnaire was used to collect information on demographic, socioeconomic, behavioral and anthropometric variables and outcomes. Crude and adjusted

  6. Comparison of Intranasal Outer Membrane Vesicles with Cholera Toxin and Injected MF59C.1 as Adjuvants for Malaria Transmission Blocking Antigens AnAPN1 and Pfs48/45

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    Michael Pritsch

    2016-01-01

    Full Text Available Purified protein vaccines often require adjuvants for efficient stimulation of immune responses. There is no licensed mucosal adjuvant on the market to adequately boost the immune response to purified antigens for intranasal applications in humans. Bacterial outer membrane vesicles (OMV are attractive candidates potentially combining antigenic and adjuvant properties in one substance. To more precisely characterize the potential of Escherichia coli OMV for intranasal vaccination with heterologous antigens, immune responses for AnAPN1 and Pfs48/45 as well as ovalbumin as a reference antigen were assessed in mice. The intranasal adjuvant cholera toxin (CT and parenteral adjuvant MF59C.1 were used in comparison. Vaccinations were administered intranasally or subcutaneously. Antibodies (total IgG and IgM as well as subclasses IgG1, IgG2a, IgG2b, and IgG3 were measured by ELISA. T cell responses (cytotoxic T cells, Th1, Th17, and regulatory T cells were determined by flow cytometry. When OMV were used as adjuvant for intranasal immunization, antibody and cellular responses against all three antigens could be induced, comparable to cholera toxin and MF59C.1. Antigen-specific IgG titres above 1 : 105 could be detected in all groups. This study provides the rationale for further development of OMV as a vaccination strategy in malaria and other diseases.

  7. Perbandingan Efektivitas Daya Hambat Kotrimoksazol Generik dan Paten terhadap Pertumbuhan Bakteri Escherichia coli sebagai Penyebab Infeksi Saluran Kemih secara In Vitro

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    Puti Anggun Sari

    2015-01-01

    Full Text Available AbstrakInfeksi saluran kemih merupakan salah satu infeksi yang sering ditemukan setelah infeksi saluran napas. Penyebab terbanyak infeksi saluran kemih adalah Escherichia coli. Kotrimoksazol merupakan kombinasi dari dua obat yaitu trimetoprim dan sulfametoksazol serta salah satu contoh antibiotik yang merupakan first-line therapy untuk infeksi saluran kemih. Kotrimoksazol terbagi menjadi dua jenis obat yaitu obat generik dan paten. Akan tetapi, belakangan ini penggunaan obat generik mulai menurun di masyarakat dan masyarakat cenderung meragukan kualitasnya. Padahal masyarakat yang akan diuntungkan jika mengetahui mutu obat generik tidak kalah dengan obat paten. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas daya hambat kotrimoksazol generik dan paten terhadap pertumbuhan bakteri Escherichia coli sebagai penyebab infeksi saluran kemih secara in vitro. Penelitian ini merupakan penelitian analitik eksperimental dengan desain cross-sectional study. Penelitian ini menggunakan 19 sampel isolat bakteri dari pasien infeksi saluran kemih yang disebabkan oleh Escherichia coli di RSUP Dr. M. Djamil yang ditentukan rumus minimal adequate sample size menggunakan metode difusi cakram dan dilihat perbandingan antara zona bebas kuman yang dibentuk oleh obat generik dan paten tersebut. Data yang diperoleh akan diolah secara statistik dengan uji hipotesis t-independent test. Hasil penelitian menunjukkan bahwa pada 17 sampel tidak didapatkan zona bebas kuman baik pada kotrimoksazol generik atau paten. Sedangkan pada 2 sampel lainnya didapatkan zona bebas kuman pada kedua obat kotrimoksazol generik dan paten. Setelah dilakukan analisis statistik menggunakan SPSS dengan uji t-independent test didapatkan bahwa tidak terdapat perbedaan bermakna antara sensitivitas bakteri Escherichia coli penyebab infeksi saluran kemih terhadap kotrimoksazol generik dan paten. Sedangkan resistensi yang terjadi pada 17 sampel lain diduga disebabkan penggunaan

  8. Studi Numerik Peningkatan Cooling Performance pada Lube Oil Cooler Gas Turbine yang Disusun Secara Seri dan Paralel dengan Variasi Kapasitas Aliran Lube Oil

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    Annis Khoiri Wibowo

    2014-09-01

    Full Text Available Salah satu komponen pada gas turbine adalah lube oil cooler yang berfungsi sebagai heat exchanger untuk mendinginkan temperatur lube oil. Pemasangan tiga lube oil cooler type-Z compact heat exchanger pada susunan seri dan paralel berdampak pada cooling capacity lube oil cooler. Uniformity flow rate pada masing-masing tube merupakan salah satu faktor yang mempengaruhi cooling capacity dari lube oil coole. Oleh karena itu dilakukan simulasi Computational Fluid Dynamic (CFD untuk mengkaji pengaruh pemasangan susunan tiga lube oil cooler secara seri dan paralel dengan variasi kapasitas lube oil terhadap performance lube oil cooler. Pemodelan domain dilakukan dengan 3 dimensi pada sisi eksternal dan internal. Simulasi pada sisi eksternal dilakukan untuk memperoleh nilai koefisien heat transfer pada masing-masing baris tube. Selanjutnya, nilai koefisien heat transfer yang didapat pada sisi eksternal digunakan sebagai kondisi batas wall convection pada masing-masing baris tube untuk simulasi internal flow dengan variasi flow rate lube oil 30 gpm, 50 gpm, 74 gpm. Dari hasil simulasi, susunan cooler seri menghasilkan cooling capacity yang lebih baik dari pada susunan cooler paralel pada kapasitas lube oil yang sama. Hal tersebut terjadi karena flow ratio lube oil untuk masing-masing tube pada susunan cooler seri lebih seragam dari pada susunan cooler paralel. Keseragaman flow rate pada masing-masing tube ditunjukkan dengan kecilnya standard deviasi flow ratio. Kapasitas 50 gpm memiliki standard deviasi flow ratio sebesar 0,46 untuk susunan seri dan 0,75 untuk susunan paralel. Semakin besar kapasitas lube oil maka distribusi flow rate pada masing-masing tube semakin tidak seragam. Selain itu susunan cooler seri memiliki pressure drop yang lebih besar dari pada susunan cooler paralel. Pemasangan susunan cooler dengan kapasitas 30 gpm memiliki tingkat keseragaman yang paling tinggi ditunjukkan dengan standard deviasi flow ratio pada masing-masing tube yang

  9. 维生素D对妊娠糖尿病胰岛素敏感性脂肪因子及肿瘤坏死因子-α的影响%Effects of Vitaimin D on Insulin Sensitivity and the Adipokine Factors APN,Visfatin, RBP4,TNF-αin Patients with Gestational Dellitus Mellitus

    Institute of Scientific and Technical Information of China (English)

    张黎明; 高凌; 帅红霞; 润袁敏; 邓晨盺; 田源; 马计; 肖婧

    2014-01-01

    Objective To investigate the relationship and mechanism of the serum vitamin D levels with insulin sensitivity and adipokines APN,RBP4,visfatin and TNF-α in patients with gestational diabetes mellitus(GDM). Methods A total of 101 patients with gestational diabetes( GDM group) and 50 pregnant women with normal blood sugar( control group) were recruited to detect the serum levels of correlative biochemical indexes,APN,RBP4,visfatin,TNF-α and 25OHD3 using an HPLC method. Twenty-four patients in the GDM group diagnosed with vitamin D deficiency(<25 nmol·L-1 ) were randomized to receive either 1,25(OH) 2D3(0. 25 μg·d-1) orally or no treatment. Serum levels of correlative biochemical indexes were detected after 1 month. Results GDM patients had 25OHD3 levels significantly lower than the control group(P<0. 05),APN levels lower than the control group,and RBP4,visfatin,TNF-α levels significantly higher than the control group(all P<0. 05). After 1 month of 1,25(OH) 2D3 treatment,HOMA-IR increased,while HOMA-β decreased. APN levels was positively correlated(r= 0. 526) with 25OHD3;RBP4,visfatin,TNF-α decreased and were negatively correlated with 25OHD3( r values were -0. 272,-0. 153,-0. 072). Conclusion Vitamin D can reduce the adipokines RBP4,visfatin,TNF-α,increase APN,and thus play a protective role for gestational diabetes.%目的:探讨血清维生素D水平与妊娠糖尿病( GDM)胰岛素敏感性及对脂肪因子脂联素( APN)、视黄醇结合蛋白4( RBP4)、内脂素(visfatin)及肿瘤坏死因子(TNF-α)的影响及作用机制。方法选择妊娠糖尿病患者101例( GDM组)和血糖正常孕妇50例(正常组),检测两组相关生化指标、APN、RBP4、visfatin、TNF-α及25羟化维生素 D3(25OHD3)。在GDM组中将诊断为维生素D缺乏患者24例随机分为治疗组及对照组各12例,治疗组口服1,25二羟维生素D3[1,25(OH)2D3]0.25μg·d-11个月,再行相关生化指标检查。结果GDM组患者25OHD3及APN水平明显低于

  10. Tratamento da síndrome da apnéia e hipopnéia obstrutiva do sono com aparelhos intrabucais Treatment of obstructive sleep apnea and hipoapnea syndrome with oral appliances

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    Marco Antonio de Oliveira Almeida

    2006-10-01

    Full Text Available A síndrome da apnéia e hipopnéia obstrutiva do sono (SAHOS é um distúrbio que atinge cerca de 4% da população adulta e que, além dos problemas sociais associados ao ronco e à sonolência diurna excessiva, é preocupante pelos quadros de hipertensão pulmonar e insuficiência cardíaca que pode desencadear. REVISÃO E DISCUSÃO: Através de uma revisão de literatura discutiu-se o uso de aparelhos intrabucais para o tratamento dessa patologia, destacando-se a eficácia e as limitações dessa terapia, os principais sintomas clínicos, os principais efeitos colaterais oclusais, o grau de colaboração e o índice de satisfação dos pacientes. CONCLUSÕES: Concluiu-se que a terapia com aparelhos intrabucais deve ser a de primeira escolha para o tratamento de SAHOS de média a moderada, sendo o desconforto dentário, articular e muscular, a hipersalivação e a xerostomia os sintomas clínicos mais freqüentes, com efeitos colaterais oclusais leves que normalmente não geram incômodos aos pacientes, com bom grau de colaboração e alto índice de satisfação.Obstructive sleep apnea and hipoapnea syndrome (OSAHS is a disorder that affects about 4% of the adult population, and besides the social problems associated to snoring and extreme day time sleepiness, it is preoccupying since it may cause pulmonary hypertension and cardiac failure. REVIEW AND DISCUSSION: Through a literature review, we discuss the use of oral appliances to treat this condition, in regards of therapy effectiveness and limitations, main clinical symptoms, major occlusal side effects, rate of improvement and patient satisfaction. CONCLUSIONS: We concluded that the use of oral appliances should be a first choice treatment for mild to moderate OSAHS, being dental, joint and muscular discomforts, hypersalivation and xerostomia, the most frequent clinical symptoms, with light occlusal side effects that normally do not bother the patients, with a good degree of improvement

  11. Uji Efek Antibakteri Minyak Jintan Hitam (Nigella Sativa Dalam Kapsul yang Dijual Bebas Selama Tahun 2012 di Kota Padang Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Secara In Vitro

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    Gezha Utama Putra

    2015-05-01

    Full Text Available AbstrakJintan Hitam atau Nigella sativa diketahui memiliki kemampuan sebagai antibakteri, seperti terhadap Staphylococcus aureus dan Escherichia coli. Kedua bakteri ini memiliki sifat yang berbeda namun memiliki kesamaan dari penyakit yang disebabkannya. Tujuan penelitian ini adalah untuk membandingkan efek antibakteri produk minyak jintan hitam (Nigella sativa dalam bentuk kapsul antara bakteri Staphylococcus aureus dan Escherechia coli secara in vitro. Penelitian ini dilakukan pada bulan Januari 2012 - Desember 2012 di Laboratorium Mikrobiologi, Fakultas Kedokteran Universitas Andalas. Penelitian ini menunjukkan bahwa semua sampel jintan hitam yang digunakan tidak memiliki efek antibakteri terhadap Escherichia coli dan semua sampel tersebut memiliki efek antibakteri yang bervariasi pada Staphylococcus aureus. Analisis Anova menunjukkan bahwa masing-masing sampel minyak Nigella sativa memberikan efek berbeda secara signifikan pada pertumbuhan Staphylococcus aureus, bahkan enam diantaranya memiliki efek yang lebih baik daripada Cepoperazon (25 mm sebagai kontrol yang digunakan.Kata kunci: Nigella sativa, efek antibakteri, Staphylococcus aureus, Escherichia coli. AbstractBlack Cumin or Nigella sativa known have the capability as antibacterial, such as to Staphylococcus aureus and Escherichia coli. Both of these bacteria have different properties but have similarities from diseases they may cause. The objective of this study was to compare anti-bacterial effects of black cumin oil (Nigella sativa which has been packaged in capsules towards Staphylococcus aureus and Escherichia coli in vitro The research was conducted in January 2012 to December 2012 at the Microbiology laboratory, Medicine Faculty of Andalas University. The results of this research indicate that all black cumin samples that are used did not have antibacterial effect against Escherichia coli and all samples had antibacterial effects on Staphylococcus aureus with germ-free area

  12. Analyse des déterminants du dépistage du syndrome d'apnées du sommeil en France : Une analyse à partir des données de l'Enquête Santé et Protection Sociale de 2008

    OpenAIRE

    Tendero, Marjorie

    2014-01-01

    Ce travail se propose d'identifier les facteurs de risques augmentant la probabilité d'être dépisté et diagnostiqué du syndrome d'apnées du sommeil (SAS). L'étude est basée sur l'enquête santé et protection sociale (ESPS) de 2008. Cette enquête a recueilli des données sur l'état de santé et l'accès aux soins des ménages résidant en France métropolitaine. L'exploitation des données collectées par cette enquête nous a permis de répondre à la problématique suivante : quelles sont les déterminant...

  13. Atuação fonoaudiológica na síndrome da apnéia e hipopnéia obstrutiva do sono: relato de caso Speech therapy in the obstructive sleep apnea-hypopnea syndrome: case report

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    Letícia Maria de Paula Silva

    2007-12-01

    Full Text Available OBJETIVO: verificar a contribuição da Fonoaudiologia no tratamento de uma paciente com a síndrome da apnéia e hipopnéia obstrutiva do sono. MÉTODOS: relato de caso de um paciente do sexo feminino, de sessenta anos de idade, com a síndrome da apnéia e hipopnéia do sono, de grau grave, que se submeteu ao atendimento fonoaudiológico para melhora do quadro de apnéia/hipopnéia, ronco e cansaço diurno. Foram realizadas avaliação clínica de motricidade orofacial e polissonografia antes e após a fonoterapia. Com base na avaliação clínica foi elaborado um plano terapêutico que buscou propiciar à paciente relaxamento cervical e da musculatura supra-hióidea, melhora na aeração nasal, adequação do posicionamento e força de língua, fortalecimento dos músculos do palato mole e sua mobilidade, aumento de força da musculatura mastigatória, treino da mastigação bilateral alternada e abaixamento do osso hióide. RESULTADOS: após 12 sessões de terapia fonoaudiológica, com duração de 40 minutos cada, pôde-se observar diminuição da tensão cervical, relaxamento da musculatura supra-hióidea, adequação do posicionamento do osso hióide, língua normotensa com dorso rebaixado, palato mole com mobilidade normal e mastigação adequada. A paciente relatou importante melhora no cansaço diurno. O resultado da segunda polissonografia indicou diminuição de 44 para 3 eventos por hora de apnéia e hipopnéia durante o sono, tendo o quadro passado do nível grave a um índice de distúrbio respiratório baixo não mais caracterizando doença apnéica do sono. CONCLUSÃO: a fonoterapia foi eficaz para o tratamento deste caso de síndrome da apnéia e hipopnéia do sono.Purpose: to check the contribution of speech therapy in the treatment of a patient with obstructive sleep apnea-hypopnea syndrome. METHODS: case description of a sixty-year old, female patient with severe obstructive sleep apnea-hypopnea syndrome that underwent

  14. Quality of life of patients with obstructive sleep apnea syndrome treated with an intraoral mandibular repositioner Qualidade de vida em pacientes com síndrome de apnéia obstrutiva do sono tratados com reposicionador mandibular intra-oral

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    Marco Antonio Cardoso Machado

    2004-06-01

    Full Text Available BACKGROUND: Obstructive sleep apnea syndrome (OSAS is a very important and prevalent disease, which is associated with a poor quality of life in many patients. Treatments for OSAS include surgery, Continuos Positive Air way Pressure, and an intraoral mandibular repositioner (IOMR, but the tendency of sleep centers is to emphasize the apnea index, neglecting quality of life as treatment outcome. OBJECTIVE: To verify to what extent treatment with an IOMR improves the OSAS patient's quality of life. METHOD: Eleven male patients aged 34 to 63 years (mean=49 with mild to moderate OSAS were evaluated using the Calgary SAQLI questionnaire applied before and four weeks after treatment with an IOMR. The mandibular repositioners were manufactured individually for each patient with acrylic polymer and equipped with a retentive device to maintain the mandible in a forward position during sleep. RESULTS: Excellent improvement in the quality of life was observed in five patients (45.5% and excellent improvement in symptoms in 10 (90.9%. CONCLUSION: The systematic use of the IOMR indicates a clear improvement in the global quality of life as well as in the symptoms of patients with OSAS.CONTEXTO:A síndrome da apnéia obstrutiva do sono (SAOS é importante e prevalente doença associada à má qualidade de vida em muitos pacientes. Os tratamentos disponíveis incluem cirurgias, CPAP e o aparelho reposicionador mandibular intra-oral (ARMIO. Muitos centros de distúrbios de sono valorizam apenas a redução do número de apnéias no tratamento da SAOS, dando pouca atenção à qualidade de vida como desfecho do tratamento. OBJETIVO: Graduar melhora da qualidade de vida dos pacientes com SAOS usando ARMIO. MÉTODO: O questionário Calgary SAQLI foi aplicado, antes e após, 4 semanas de tratamento com ARMIO, a 11 pacientes masculinos, entre 34 e 63 anos de idade (média de 49 anos, com SAOS leve a moderada. ARMIOs foram manufaturados com polímero acrílico e

  15. Mouth breathing children have cephalometric patterns similar to those of adult patients with obstructive sleep apnea syndrome Crianças respiradoras bucais apresentam padrão cefalométrico semelhante àquele observado em pacientes adultos com síndrome da apnéia obstrutiva do sono

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    Maria Ligia Juliano

    2009-09-01

    Full Text Available OBJECTIVE: To determine whether mouth breathing children present the same cephalometric patterns as patients with obstructive sleep apnea syndrome (OSAS. METHOD: Cephalometric variables were traced and measured on vertical lateral cephalometric radiographs. The cephalometric measurements of 52 mouth and 90 nose breathing children were compared with apneic patients. The children had not undergone adenoidectomy or tonsillectomy and had not had or were not receiving orthodontic or orthopedic treatment. RESULTS: Mouth breathing children showed same cephalometric pattern observed in patients with OSAS: a tendency to have a retruded mandible (p=0.05, along with greater inclination of the mandibular and occlusal planes (pOBJETIVO: Determinar se crianças respiradoras bucais apresentam o mesmo padrão cefalométrico que os pacientes que tem síndrome da apnéia obstrutiva do sono (SAOS. MÉTODO: Foram traçadas radiografias laterais verticais da cabeça para a mensuração das variáveis cefalométricas. As medidas cefalométricas de 52 crianças respiradoras bucais e de 90 crianças respiradoras nasais foram comparadas à de pacientes com apnéia. Foram excluídas as crianças que haviam sido submetidas à cirurgia de remoção de amídalas ou adenóides, ou que haviam recebido tratamento ortodôntico prévio ou em andamento. RESULTADOS: As crianças respiradoras bucais apresentaram o mesmo padrão cefalométrico observado em pacientes com SAOS: tendência a ter retrusão de mandíbula (p=0,05, assim como uma maior inclinação dos planos mandibular e oclusal (p<0,01 e tendência a ter maior inclinação dos incisivos superiores (p=0,08. O espaço da nasofaringe e o espaço aéreo posterior se apresentaram muito diminuídos nas crianças respiradoras bucais, como é observado em pacientes com SAOS (p<0,01. CONCLUSÃO: Crianças respiradoras bucais apresentam padrão cefalométrico alterado e sua morfologia craniofacial é semelhante àquela observada

  16. Pengolahan Limbah Cair Rumah Sakit secara Sonochemical

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    Laila Kusuma

    2017-06-01

    Full Text Available Hospital as a service provider has an important role in human life, especially for people who are experiencing physical and mental disorders. Various types of diseases and therapies performed in hospitals has made it as one of the producers of hazardous and infectious waste that requires special handling and processing so as not to pollute the water, soil, and air environment. Hospital wastewater treatment was conducted to determine the ability of sonochemical method to reduce level of Chemical Oxygen Demand (COD and determine the optimum conditions of the parameters used by the design of Response Surface Methodology Box-Behnken. The parameters that affected this process was the concentration of hydrogen peroxide (H2O2, pH, and reaction time. The Waste used was taken from the waste water treatment plant in Zainoel Abidin General Hospital in Banda Aceh with COD value 260.65 ppm. The optimum conditions of this treatment process occured at pH 2, H2O2 concentration 500 ppm, and reaction time 119.98 minutes with reduction of COD was 81.88%.ABSTRAKRumah sakit sebagai penyedia layanan jasa memegang peranan cukup penting dalam kehidupan manusia, terutama bagi orang-orang yang sedang mengalami gangguan fisik dan mental. Berbagai jenis penyakit dan terapi yang dilakukan di rumah sakit telah menjadikannya sebagai salah satu penghasil limbah berbahaya dan infeksius yang memerlukan penanganan dan pengolahan khusus agar tidak mencemari lingkungan yaitu air, tanah dan udara. Pengolahan limbah cair rumah sakit dilakukan untuk mengetahui kemampuan metode sonochemical dalam menurunkan kadar Chemical Oxygen Demand (COD dan menentukan kondisi optimum dari parameter yang digunakan berdasarkan disain Response Surface Methodology Box-Behnken. Parameter yang mempengaruhi proses ini adalah konsentrasi hidrogen peroksida (H2O2, pH, dan waktu reaksi. Limbah yang digunakan diambil dari instalasi pengolahan air limbah di Rumah Sakit Umum Zainoel Abidin Kota Banda Aceh dengan nilai COD sebesar 260,65 ppm. Kondisi optimum dari proses pengolahan terjadi pada pH 2, konsentrasi H2O2 500 ppm, dan waktu reaksi 119,98 menit dengan penurunan COD sebesar 81,88 %

  17. Pembelajaran Interdisipliner: Upaya Mengapresiasi Sastra Secara Holistik

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    Daru Kabeka Janarto

    2010-10-01

    Full Text Available The research is regarding 4 class cycle about interdisciplinary teaching technique of literature teaching in BINUS International School Simprug, Jakarta. The interdisplinary teaching is done in grade 10 in 3 subjects of Indonesian Language and Literature, Computer (IT, and Visual Arts. The teaching topic is Ma Yan, a novel about Muslim teenager who struggles for education in Zhang Jiashu, a remote village in China. This teaching climax is talk show with the writer of Ma Yan and its publisher followed with visual arts show from the students. The observation result shows that talent and involvement of students are high through the learning materials, proved by assignments done in time and their antusiasm towards their projects and assignments.  

  18. PEMBELAJARAN INTERDISIPLINER: UPAYA MENGAPRESIASI SASTRA SECARA HOLISTIK

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    Daru Kabeka Janarto

    2010-09-01

    Full Text Available The research is regarding 4 class cycle about interdisciplinary teaching technique of literature teaching in BINUS International School Simprug, Jakarta. The interdisplinary teaching is done in grade 10 in 3 subjects of Indonesian Language and Literature, Computer (IT, and Visual Arts. The teaching topic is Ma Yan, a novel about Muslim teenager who struggles for education in Zhang Jiashu, a remote village in China. This teaching climax is talk show with the writer of Ma Yan and its publisher followed with visual arts show from the students. The observation result shows that talent and involvement of students are high through the learning materials, proved by assignments done in time and their antusiasm towards their projects and assignments.

  19. PERANCANGAN MESIN PENYAYAT BAMBU SECARA ERGONOMIS

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    Agung Kristanto

    2012-12-01

    Full Text Available Jamboel Kipas adalah UKM yang memproduksi souvenir kipas dengan sistem pesanan make to order. Seiring perkembangan waktu, Jamboel Kipas tidak dapat memenuhi pesanan karena keterbatasan kapasitas produksi souvenir kipas. Hal ini disebabkan karena waktu proses yang lama terjadi pada bagian penyayatan bambu, yaitu sebesar 22 detik/iratan. Penelitian ini ditujukan untuk meningkatkan produktivitas penyayatan bambu serta memperbaiki posisi operator saat bekerja, dengan perancangan mesin penyayat bambu berdasar anthropometri ukuran tubuh operator, keluhan selama bekerja, dan waktu proses penyayatan bambu. Hasil penelitian setelah perancangan menunjukan posisi operator saat bekerja cukup ergonomis karena ukuran tempat kerja disesuaikan dengan dimensi antropometri operator. Waktu baku pada kondisi sebelum perancangan sebesar 21,16 detik/iratan dan output standar sebesar 170,09 iratan/jam. Sedangkan setelah perancangan sebesar 4,42 detik/iratan dan output standarnya sebesar 815,22 iratan/jam dengan peningkatan output standar 479,46%, terjadi penurunan waktu baku sebesar 378,73%. Sementara itu nilai break event point terjadi ketika UKM Jamboel Kipas sudah memproduksi 12927 kipas.

  20. PERANCANGAN MESIN PENYAYAT BAMBU SECARA ERGONOMIS

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    Agung Kristanto

    2012-12-01

    Full Text Available Jamboel Kipas adalah UKM yang memproduksi souvenir kipas dengan sistem pesanan make to order. Seiring perkembangan waktu, Jamboel Kipas tidak dapat memenuhi pesanan karena keterbatasan kapasitas produksi souvenir kipas. Hal ini disebabkan karena waktu proses yang lama terjadi pada bagian penyayatan bambu, yaitu sebesar 22 detik/iratan. Penelitian ini ditujukan untuk meningkatkan produktivitas penyayatan bambu serta memperbaiki posisi operator saat bekerja, dengan perancangan mesin penyayat bambu berdasar anthropometri ukuran tubuh operator, keluhan selama bekerja, dan waktu proses penyayatan bambu. Hasil penelitian setelah perancangan menunjukan posisi operator saat bekerja cukup ergonomis karena ukuran tempat kerja disesuaikan dengan dimensi antropometri operator. Waktu baku pada kondisi sebelum perancangan sebesar 21,16 detik/iratan dan output standar sebesar 170,09 iratan/jam. Sedangkan setelah perancangan sebesar 4,42 detik/iratan dan output standarnya sebesar 815,22 iratan/jam dengan peningkatan output standar 479,46%, terjadi penurunan waktu baku sebesar 378,73%. Sementara itu nilai break event point terjadi ketika UKM Jamboel Kipas sudah memproduksi 12927  kipas.

  1. BELAJAR BAHASA SECARA HOLISTIK: APAKAH PANDANGAN MURID ?

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    Zulkifli Osman

    2013-10-01

      Learning Language by Holistic Approach: What Atudent’s View”. A variety of teaching approaches such as structural approach, code-cognitive, communicative was implemented in the classroom. These approaches have their own specific strength and weakness. The cod-cognitive approach emphasizes on the ability of the students to mastering the language competence. The communicative approach likewise to enhance on the communication process only. The purpose of this study is to introduce the implementation of the holistic approach in teaching of the Malay Language to ensure pupils are able to learn the language as a whole or holistic. In order to obtain the student’s views on the implementation of this approach, questionnaire and semi-structured interview was implemented. Document analysis also used in order to support the data. One hundred and twenty five students are involved in this study whose consist of three classes of Form Four (SMA. Questionnaires and interviews were administered to the students after teaching modules run. Results showed that the language process by using the holistic approach is more attractive. This approach is able to increase the student’s motivation and language skills. Implications of the study are also discussed Keywords: Learning language, language learning, holistic approach,                   language teaching modules

  2. Perawatan Kasus Diastema Multipel Secara Multidisiplin

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    Fadli Jazaldi

    2012-10-01

    Full Text Available Diastemas are spaces or gaps between two or more consecutive teeth. They can occur anywhere in the upper or lower dental arches. Diastemas can occur in mixed dentition and closes spontaneously when the canines erupt, but in certain circumstances, diastema will persist until period of permanent teeth. This etiologic factor has to identify because it will influenc effectivenees of closing the multiple diastemas. The multiple diastemas that happened at the anterior upper and lower which caused by deficient tooth structure and missing of some teeth (agenesis, accompanied of central diastema which influenced by anterior labialis frenulum, should treated comprehensively. Treatment of this case entangle ortodontic, periodontic, conservative dentistry, and prostodontic. Reposition of abnormal frenulum attachment (frenectomy, arrangement of occlusion and teeth interdigitation, getting continuity of dental arch through achieving good approximal teeth contact by crown reshaping andreplaced of missing tooth element with prothesis, and then making of retainer to maintain stability of treatment result are serial of treatments for the case. By cooperation of multidicipline proffesionals, the final result is to obtain the optimal esthetics, functional, and stability result of  treatment, will achived.DOI: 10.14693/jdi.v15i3.29

  3. Perbedaan Efek Daya Hambat Jus Kulit Buah Manggis dengan Air Rebusan Kulit Buah Manggis sebagai Antibakteri terhadap Bakteri Gram-Positif (Staphylococcus aureus dan Streptococcus pyogenes secara In Vitro

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    Nani Hendriani

    2016-01-01

    Full Text Available AbstrakBakteri Gram-positif seperti Staphylococcus aureus dan Streptococcus pyogenes telah resisten terhadap beberapa antibiotik, sehingga perlu dicari antibakteri alternatif lain. Manggis merupakan salah satu tanaman obat tradisional yang telah lama digunakan sebagai antibakteri. Penelitian ini dilakukan secara eksperimental terhadap kedua bakteri tersebut,  yang diberi enam perlakuan dengan enam kali pengulangan, yaitu kontrol positif (amoksisilin 25 mcg, kontrol negatif (larutan aquades, jus kulit buah manggis dosis I (konsentrasi 58,3% v/v dan dosis II (konsentrasi 29,15% v/v, serta air rebusan kulit buah manggis dosis I (konsentrasi 30,7% v/v dan dosis II (konsentrasi 15,35% v/v. Cawan petri dengan kedua isolat bakteri  yang telah ditanami cakram dengan 6 perlakuan diinkubasi selama 24 jam pada suhu 37°C, kemudian diukur diameter halo yang dihasilkan. Hasil penelitian menunjukkan bahwa air rebusan kulit buah manggis memiliki efek daya hambat yang lebih baik daripada jus kulit buah manggis. Efek antibakteri jus dan air rebusan kulit buah manggis lebih sensitif pada bakteri Staphylococcus aureus dibanding bakteri Streptococcus pyogenes.Kata kunci: kulit buah manggis, jus, air rebusan, Staphylococcus aureus, Streptococcus pyogenes AbstractGram-positive bacteria such as Staphylococcus aureus and Staphylococcus pyogenes have developed resistance to some antibiotic, therefore  need to find another alternative as antibacterial. Mangosteen well known as one of the traditional medicine used as antibacterial. This study was conducted in experimental fashion toward both of those bacteria which was given 6 treatment with 6 times repetition, consist of positive control (25 mcg of amoxicillin, negative control (aquades solution, mangosteen pericarp juice dose I (58% v/v and dose II (29,15% v/v, and boiled mengosteen pericarp dose I (30,7% v/v and dose II (15,35% v/v. Six paper disks treated with before mentioned treatment was putted on a petri dish

  4. KEANEKARAGAMAN JENIS TUMBUHAN YANG BERGUNA SECARA LOKAL DI DESA BATU AMPAR, DI DEKAT KAWASAN HUTAN LINDUNG BUKIT RAJA MANDARA, KABUPATEN BENGKULU SELATAN (The Diversity of Locally Useful Plants in Batu Ampar Village Near Bukit Raja Mandara Protected

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    Wiryono Wiryono

    2013-07-01

    Full Text Available ABSTRAK Keanekaragaman jenis tumbuhan memiliki banyak fungsi bagi masyarakat, terutama adalah mereka yang tinggal di daerah pedesaan. Masing-masing masyarakat tradisional di Indonesia memiliki pengetahuan khusus tentang tumbuhan yang terdapat di lingkungan mereka dan pemanfaatanya. Memiliki banyak jenis tumbuhan dan suku bangsa, Indonesia kaya akan keanekaragaman biologi dan budaya. Namun, pertanian modern dan globalisasi cenderung menurunkan keanekaragaman biologi dan budaya tersebut. Oleh karena itu, pelestarian pengetahuan dan pemanfaatan tradisional sangat penting untuk dilakukan. Penelitian ini bertujuan untuk mendokumentasikan keanekaragaman jenis tumbuhan yang digunakan oleh penduduk desa Batu Ampar, di Kecamatan Kedurang, Kabupaten Bengkulu Selatan. Hasil penelitian menunjukkan bahwa penduduk desa tersebut memanfaatkan 83 jenis tanaman dalam 8 kategori, yaitu bahan makanan sebanyak 35 jenis, bahan obat-obatan 30 jenis, bahan bangunan 16 jenis, kayu bakar 9 jenis, pagar dan pagar hidup 9 jenis, kerajinan tangan 9 jenis, pewarna 1 jenis dan racun 1 jenis. Lima puluh lima jenis tumbuhan diambil dari lahan pribadi dan 28 jenis dari hutan. Secara ekonomis, 54 jenis tumbuhan dimanfaatkan untuk keperluan sendiri, sedangkan 29 jenis tumbuhan selain dimanfaatkan sendiri juga dijual. ABSTRACT The diversity of plant species serves many purposes for community, especially those living in rural areas. Each traditional community inIndonesiamay have specific knowledge and use of plants found in their environment.  Having many plant species and tribes,Indonesiais rich in biological and cultural diversity. Modern agriculture and globalization, however, tends to reduce both diversities. It is, therefore, essential that traditional knowledge and uses of biological diversity be preserved. This study was aimed to document the diversity of plants used by villagers of Batu Ampar,KedurangSubdistrict, South Bengkulu District. Results showed that villagers used

  5. Estudo comparativo das seqüências rápidas ponderadas em T2, utilizando-se sincronização respiratória, apnéia, supressão de gordura, bobina de corpo e bobina de sinergia para a avaliação do fígado pela ressonância magnética

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    Abbehusen Cristiane L.

    2003-01-01

    Full Text Available OBJETIVO: Comparar, qualitativa e quantitativamente, as imagens de ressonância magnética do fígado, ponderadas em T2, utilizando-se seqüências rápidas, diferenciadas pela técnica de controle respiratório, pela utilização de supressão de gordura e pelo tipo de bobina de radiofreqüência. MATERIAIS E MÉTODOS: Estudo prospectivo em 71 pacientes consecutivos, sendo realizadas seis seqüências para comparação: 1 supressão de gordura com sincronização respiratória e bobina de corpo; 2 supressão de gordura em apnéia e bobina de corpo; 3 sem supressão de gordura com sincronização respiratória e bobina de corpo; 4 sem supressão de gordura em apnéia e bobina de corpo; 5 com supressão de gordura com sincronização respiratória e bobina de sinergia; 6 com supressão de gordura em apnéia e bobina de sinergia. A avaliação qualitativa foi baseada em três critérios: detecção de determinadas estruturas anatômicas do fígado, definição dos contornos hepáticos, e presença de artefatos de respiração. A análise quantitativa foi obtida através da relação das intensidades de sinal do fígado e do ruído de fundo. RESULTADOS: O valor médio dos índices globais de qualidade de imagem para cada uma das seis seqüências supracitadas foi de 7,8, 4,6, 7,9, 5,2, 6,7 e 4,6, respectivamente. As seqüências obtidas com sincronização respiratória apresentaram melhor qualidade de imagem e relação sinal/ruído superiores às seqüências com apnéia (p 0,05. As seqüências obtidas com bobina de sinergia apresentaram qualidade de imagem semelhante (p > 0,05 e relação sinal/ruído inferior àquelas com bobina de corpo (p < 0,001. CONCLUSÃO: Associando-se as análises qualitativa e quantitativa das imagens, as melhores seqüências foram aquelas obtidas com sincronização respiratória e bobina de corpo, utilizando-se ou não supressão de gordura.

  6. Efeitos da pressão positiva contínua em vias aéreas sobre os sintomas nasofaríngeos em pacientes com a síndrome da apnéia obstrutiva do sono Effects of continuos positive airway pressure on nasal and pharyngeal symptoms in patients with obstructive sleep apnea

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    Adelaide Cristina de Figueiredo

    2004-12-01

    Full Text Available INTRODUÇÃO: Sintomas nasofaríngeos são comuns em pacientes com a síndrome da apnéia obstrutiva do sono (SAOS em tratamento com pressão positiva contínua em vias aéreas (CPAP. No entanto, sintomas nasofaríngeos são também comuns em pacientes com SAOS antes do início do tratamento. OBJETIVO: Determinar o impacto do tratamento com CPAP nasal sobre os sintomas nasofaríngeos em pacientes com SAOS. MÉTODO: Foram avaliados 35 pacientes (28 homens, com idade de 54 ±10 anos portadores de SAOS moderada a grave diagnosticada através de polissonografia. Os sintomas nasofaríngeos (espirros, coriza, prurido, obstrução, sangramento e ressecamento nasal e de garganta foram quantificados através de questionário aplicado antes e depois de pelo menos 3 meses de tratamento com CPAP nasal. RESULTADOS: O índice de apnéia + hipopnéia foi de 50±25 eventos por hora. Ao menos um sintoma nasofaríngeo estava presente em 26 pacientes (74% antes do tratamento. A obstrução nasal foi o sintoma mais comum, presente em 18 pacientes (51%. Dentre os pacientes inicialmente assintomáticos (n = 9, 78% apresentaram alguma reação nasofaríngea adversa com o tratamento. Em contraste, nos pacientes inicialmente sintomáticos, houve redução significativa da intensidade da obstrução, do ressecamento nasal e de garganta e do sangramento nasal após o tratamento. CONCLUSÃO: Sintomas nasofaríngeos são freqüentes em pacientes com SAOS. O uso de CPAP pode tanto desencadear sintomas nasofaríngeos em pacientes assintomáticos, como reduzir sua intensidade nos pacientes com sintomas prévios.BACKGROUND: Nasal and pharyngeal symptoms are common in patients with obstructive sleep apnoea (OSA treated with nasal continuous positive airway pressure (CPAP. However, these symptoms are common in OSA patients even before the treatment. OBJECTIVE: Determine the impact of nasal CPAP on nasal and pharyngeal symptoms in OSA patients. METHOD: Thirty-five adult patients

  7. Avaliação da escala de Epworth em pacientes com a Síndrome da apnéia e hipopnéia obstrutiva do sono Evaluation of Epworth Sleepiness Scale in patients with obstructive sleep apnea-hypopnea syndrome

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    Letícia Boari

    2004-12-01

    Full Text Available A síndrome da apnéia e hipopnéia obstrutiva do sono (SAHOS é, atualmente, considerada um problema de saúde pública por causar aumento da morbi-mortalidade cardiovascular e acidentes de trânsito. A polissonografia assistida é o padrão-ouro para o diagnóstico e acompanhamento destes pacientes. No entanto, por ser onerosa, demorada e de acesso restrito, outros métodos tem sido desenvolvidos. A escala de sonolência de Epworth (ESE é uma avaliação subjetiva, porém, rápida, sem custos e simples de ser aplicada. OBJETIVO: Avaliar a correlação entre a pontuação da ESE e o índice de apnéia e hipopnéia (IAH da polissonografia de pacientes com SAHOS. FORMA DE ESTUDO: Clínico retrospectivo. MATERIAL E MÉTODO: Revisão de prontuário de 66 pacientes com queixa de roncopatia que foram submetidos a procedimento cirúrgico (uvulopalatofaringoplastia com ou sem abordagem nasal. Avaliaram-se a pontuação da ESE e o IAH da polissonografia pré e pós-operatórios. RESULTADOS: 78,7% pacientes com grau normal de IAH tiveram pontuação de ESE menor do que 10 e 65% pacientes com grau severo de IAH tiveram pontuação maior do que 10. Não houve resultados estatisticamente significantes para os grupos moderado e leve. CONCLUSÃO: A escala de Epworth pode distinguir os graus normais e severos sem, no entanto, determinar os graus moderado e leve. Assim, pode ser utilizada para acompanhamento de pacientes com SAHOS sem, no entanto, substituir a polissonografia uma vez que não consegue avaliar todos os graus de severidade.Today obstructive sleep apnea–hypopnea syndrome (OSAHS is a public health issue, since it increases cardiovascular morbidity-mortality rate and the risk of car crashes. Overnight polysomnography is the gold standard for diagnosis and follow-up of affected patients. However, because the test is expensive, time-consuming and of difficult access, others methods have been proposed. Although the Epworth Sleepiness Scale (ESS is

  8. Efeitos sistêmicos da hipoxemia noturna em pacientes com doença pulmonar obstrutiva crônica sem síndrome da apnéia obstrutiva do sono Systemic effects of nocturnal hypoxemia in patients with chronic obstructive pulmonary disease without obstructive sleep apnea syndrome

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    Paulo de Tarso Guerrero Mueller

    2008-08-01

    Full Text Available OBJETIVO: Estudar os efeitos da hipoxemia noturna em pacientes com doença pulmonar obstrutiva crônica sem síndrome da apnéia obstrutiva do sono. MÉTODOS: Estudamos 21 pacientes-10 dessaturadores e 11 não-dessaturadores-submetidos a gasometria arterial, polissonografia, espirometria, teste de exercício cardiopulmonar (cicloergômetro, dinamometria manual e medidas de pressão inspiratória máxima, pressão expiratória máxima e proteína C reativa (PCR. Incluíram-se os pacientes com pressão parcial arterial de oxigênio > 60 mmHg; excluíram-se os com índice de apnéia-hipopnéia > 5 eventos/hora de sono. Foram medidos consumo máximo de oxigênio, potência máxima, pressão arterial sistólica, pressão arterial diastólica (PAD e frequência cardíaca máxima durante exercício, visando detectar alterações hemodinâmicas. A PCR foi considerada positiva quando acima de 3 mg/L. RESULTADOS: A saturação periférica de oxigênio mínima durante o sono foi significativamente maior nos não-dessaturadores (p = 0,03. Mais dessaturadores apresentaram PCR > 3 mg/L (p OBJECTIVE: To study the effects of nocturnal hypoxemia in patients with chronic obstructive pulmonary disease without obstructive sleep apnea syndrome. METHODS: We studied 21 patients-10 desaturators and 11 nondesaturators-submitted to arterial blood gas analysis, polysomnography, spirometry, cardiopulmonary exercise testing (cycle ergometer, and hand-grip dynamometry, as well as measurements of maximal inspiratory pressure, maximal expiratory pressure, and C-reactive protein (CRP levels. Patients with arterial oxygen tension > 60 mmHg were included; those with an apnea-hypopnea index > 5 events/hour of sleep were excluded. Maximal oxygen uptake, maximal power, systolic blood pressure, diastolic blood pressure (DBP, and maximal heart rate were measured during exercise in order to detect hemodynamic alterations. Patients presenting CRP levels above 3 mg/L were considered CRP

  9. Pemodelan Matematik Kinerja Pengering Surya Efek Rumah Kaca (ERK-Hibrid Menggunakan Rak Berputar secara Vertikal (Mathematical Modeling Performance of Greenhouse Effect (GHE-Hybrid Solar Dryer with a Vertical Rotating Rack

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    Sigit Triwahyudi

    2016-12-01

    ini menyajikan uji unjuk kerja dan pemodelan matematik pengering energi surya (ERK-Hibrid  menggunakan  rak berputar secara vertikal. Pengering terdiri dari bangunan rumah kaca berukuran (1100 × 860 × 1300 mm dengan delapan buah rak dan sistim pemanas tambahan yang terdiri dari kolektor plat datar (1,04 m2, tangki penyimpan air panas (197 liter dan penukar panas. Untuk mengetahuikinerja pengering energi surya, dilakukan lima kali percobaan variasi kecepatan putar rak (1, 4, 7, 10 rpm dan tanpa pemutaran rak. Bahan yang dipergunakan pada pengujian adalah kapulaga lokal (Amomum cardamomum Wild. Masing-masing percobaan seberat 9 sampai dengan 10 kg. Rata-rata suhu air tangki penyimpan panas bervariasi dari 50,0 sampai dengan 55,0 °C suhu ruang pengering antara 38,9 sampai dengan 45,9 °C, sedangkan kelembaban relatif (RH ruang pengering berkisar antara 32,1 sampai dengan 47,4 %. Perlakuan pemutaran rak berpengaruh terhadap keseragaman suhu bahan maupun kadar air pada masing rak pengering. Pada perlakuan pemutaran 1 rpm (percobaan II, didapatkan kondisi terbaik (suhu bahan maupun kadar air yang paling seragam. Dengan kecepatan putar rak yang semakin meningkat menyebabkan nilai ragam suhu bahan maupun kadar air semakin meningkat. Untuk melakukan simulasi unjuk kerja dilakukan pemodelan matematik berdasarkan pindah panas dan massa serta pendekatan pengeringan lapisan tipis. Persamaan diselesaikan secara numerik menggunakan finite difference Euler dengan bantuan bahasa pemrograman Visual Basic Aplication (VBA Excell. Validasi dilakukan dengan membandingkan antara perhitungan hasil pemodelan (data prediksi dengan data hasil pengukuran (data observasi. Kriteria kevalidan ditentukan dengan melihat nilai koefisien determinasi (R2, nilai RMSD maupun nilai MAPD. Pemodelan  matematik yang telah dibangun dapat menggambarkan dengan tepat perubahan suhu air tangki penyimpan panas, suhu ruang pengering, suhu bahan maupun penurunan kadar air. Kata kunci: Pengering surya efek rumah

  10. Excessive daytime sleepiness of the Brazilian emperor Dom Pedro II probably due to sleep apnea syndrome Sonolência diurna excessiva de Dom Pedro II do Brasil devida provavelmente à síndrome de apnéia do sono

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    Rubens Reimão

    2008-01-01

    Full Text Available OBJECTIVE: To show that the obstructive sleep apnea syndrome (OSAS was the probable cause of D. Pedro II's excessive daytime sleepiness. METHOD: Research of historical documents and bibliographical. RESULTS: The excessive daytime sleepiness of D. Pedro II (1825-1891 was well known and bitterly criticized behavior by oppositionist magazines; it was also recognized by his peers. He would fall asleep in public places such as the theater and while attending lectures. As a youth, he was of normal complexion, putting on weight (obesity at middle years. CONCLUSION: The possibility of this diagnosis is particularly relevant in this case because it points to an organic cause for D. Pedro II daytime naps and excessive daytime sleepiness. It could be the result of OSAS and not "disinterest" as erroneously assumed at that time.OBJETIVO: Evidenciar a síndrome de apnéia do sono tipo obstrutivo (SASO como provável causa da sonolência diurna excessiva de D. Pedro II. MÉTODO: Pesquisa de documentos históricos e bibliográfica. RESULTADOS: A sonolência diurna excessiva de D. Pedro II (1825-1891 era bem conhecida e criticada enfaticamente pelas revistas oposicionistas, era também reconhecida pelos seus pares. Ele adormecia em lugares públicos como no teatro e ao assistir aulas. Como jovem ele tinha compleição normal, ganhando peso (obesidade na meia idade. CONCLUSÃO: A possibilidade deste diagnóstico é particularmente relevante neste caso porque aponta para uma causa orgânica para os cochilos diurnos e a sonolência diurna excessiva. Pode se dever à SASO e não ao "desinteresse" como erroneamente admitido naquela época.

  11. KAJIAN ISOLAT BAKTERI ASAM LAKTAT DALAM MENURUNKAN KOLESTEROL SECARA IN VITRO DENGAN KEBERADAAN OLIGOSAKARIDA (A Study In Vitro of Lactic Acid Bacteria (LAB Isolates on Cholesterol Lowering Ability in The Presence of Oligosaccharides

    Directory of Open Access Journals (Sweden)

    Yati Maryati

    2016-10-01

    acid bacteria (LAB, oligosaccharides, synbiotic, cholesterol reduction, prebiotics ABSTRAK Penelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS, fruktooligosakarida (FOS, inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada

  12. Estudo comparativo da história clínica e da polissonografia na síndrome da apnéia/ hipopnéia obstrutiva do sono Comparative study between clinical history and polysomnogram in the obstructive sleep apnea/ hypopnea syndrome

    Directory of Open Access Journals (Sweden)

    Lys Maria Allenstein Gondim

    2007-12-01

    Full Text Available O reconhecimento dos distúrbios respiratórios do sono tem aumentado a cada ano. Manifestações, como o ronco, consideradas meros incômodos vêm adquirindo importância no que diz respeito à qualidade de vida e seu impacto social. OBJETIVO: Comparar a história clínica com os resultados da polissonografia (PSG, na Síndrome da Apnéia/Hipopnéia Obstrutiva do Sono (SAHOS, é o principal objetivo deste trabalho. MATERIAL E MÉTODOS: Foi realizado um estudo retrospectivo, com 125 pacientes, através da análise de questionários específicos, IMC e Escala de Epworth. RESULTADOS: Dentre os pacientes, 75 eram do sexo masculino e 50 do feminino. O principal sintoma foi a roncopatia. 46% apresentaram PSG normais, 30% SAHOS leve, 15% moderada e 9% severa, não se evidenciando correlação estatística entre a clínica e a PSG. Dentre as queixas, somente a insônia foi relevante, em análise univariada e em pacientes normais e com SAHOS leve (pRecognizing sleep-disordered breathing is on the rise every year. Manifestations, such as snoring, that were earlier considered mere inconvenients are now acquiring greater importance concerning life quality and social impact. AIM OF THE STUDY: To compare the clinical history to polysomnogram (PSG results in the Obstructive Sleep Apnea/Hypopnea Syndrome (OSAHS. MATERIALS AND METHODS: 125 patients were analyzed, in a retrospective study. Specific questionnaires, avaliations of Body Mass Index and Epworth Scale were carried out. RESULTS: Among the patients, 75 were males and 50 were females. The main symptom was snoring. 46% had normal PSG, 30% had light OSAHS, 15% moderate and 9% severe OSAHS and it was not observed a correlation between clinical data and PSG results. Concerning clinical symptoms, only insomnia has shown relevance when univariably analyzed in normal and light OSAHS patients (p<0,05 compared to patients with moderate and severe OSAHS, losing its importance when analyzed together with other

  13. Investigation on seismic characteristic in Muria Peninsula to asses the NPP seismic design

    Energy Technology Data Exchange (ETDEWEB)

    Kusnowo, A. [National Atomic Energy Agency, Jakarta (Indonesia)

    1997-03-01

    A feasibility study on first nuclear power plant was conducted during 4,5 years started on 22 November 1991. This study consists of two parts. First, the non site study, Second part is the site and environmental study. The scope of non site studies are economic financing, technical and safety aspect as well as fuel cycle and waste management aspect. The site and environmental studied consist of site conditions and qualification, seismological, environmental condition as well as social economic and cultural impact. In the first step of site study (step 1), the result come up to the three candidates named Ujung Lemahabang, Ujung Watu and Ujung Grenggengan. Further study on geology, topography, oceanography, geophysics, hydrology, seismology, vulcanology, man induced event, etc was done on those three candidates (named as step 2). The results come up with Ujung Lemahabang as the best candidates. It is important to know basic, characteristic of seismicity of nuclear power plant sitting region for seismic hazard assessment this was done as step 3. This paper describe the results of step 3. (J.P.N.)

  14. The " Bison Horn " Muria: Making it " More Tribal " for a Folk Dance Competition in Bastar, Chhattisgarh

    National Research Council Canada - National Science Library

    Prévôt, Nicolas

    2014-01-01

    ... of the ādivāsīs, the local authorities organizing " tribal " dance competitions, some local NGOs setting up workshops and selling " tribal handicrafts, " or the new government planning its tourism policy...

  15. Apnéia na sala de recuperação pós-anestésica: relato de caso Apnea en la sala de recuperación pós-anestésica: relato de caso Apnea in the postanesthetic recovery room: case report

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    Joel Massari Rezende

    2003-06-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A depressão respiratória é uma complicação que pode ocorrer no pós-operatório quando se utilizam opióides na anestesia. O objetivo deste relato é discutir um caso de apnéia em paciente que chegou consciente à sala de recuperação pós-anestésica (SRPA, após ter sido submetido à tireoidectomia sob anestesia geral com propofol, fentanil e isoflurano. RELATO DO CASO: Paciente do sexo feminino, 50 anos, 60 kg, estado físico ASA I, submetida à tireoidectomia sob anestesia geral induzida com propofol (140 mg, fentanil (350 µg, atracúrio (30 mg e mantida com isoflurano, duas doses subseqüentes em bolus de atracúrio (10 mg cada e ventilação controlada mecânica. No final da cirurgia, após antagonização do bloqueio neuromuscular, a paciente foi extubada, obedeceu aos comandos para respirar e colaborou na passagem à maca, sendo transportada para a SRPA, aonde chegou consciente. Minutos após, apresentou apnéia, cianose e inconsciência. Foi realizada ventilação manual com oxigênio a 100% seguida de injeção de naloxona (0,2 mg por via venosa, havendo retorno da ventilação espontânea e da consciência. CONCLUSÕES: Os cuidados ventilatórios no pós-operatório, durante o transporte, admissão à SRPA e permanência nessa unidade, devem ser contínuos em pacientes que receberam opióides, mesmo demonstrando estar conscientes ao deixarem a sala cirúrgica.JUSTIFICATIVA Y OBJETIVOS: La depresión respiratoria es una complicación que puede ocurrir en el pós-operatorio cuando se utilizan opioides en la anestesia. El objetivo de este relato es discutir un caso de apnea en paciente que llegó consciente a la sala de recuperación pós-anestésica (SRPA, después de haber sido sometido a tireoidectomia sobre anestesia general con propofol, fentanil e isoflurano. RELATO DE CASO: Paciente del sexo femenino, 50 años, 60 kg, estado físico ASA I, sometida a la tireoidectomia sobre anestesia general

  16. Prevalência de fatores de risco para a síndrome da apnéia obstrutiva do sono em motoristas de ônibus interestadual Prevalence of risk factors for obstructive sleep apnea syndrome in interstate bus drivers

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    Carlos Alberto de Assis Viegas

    2006-04-01

    Full Text Available OBJETIVO: Verificar a prevalência de fatores de risco para a síndrome da apnéia obstrutiva do sono em motoristas de ônibus interestadual. MÉTODOS: Foram avaliados 262 motoristas profissionais de ônibus interestadual, de empresa brasileira baseada no Distrito Federal, por questionário para avaliar distúrbios respiratórios do Sono, Escala de Sonolência de Epworth, testes de atenção concentrada e difusa, e medidas antropométricas. RESULTADOS: Encontravam-se com o peso acima do ideal 68% da amostra estudada, dos quais 34% apresentaram circunferência do pescoço = 42 cm. Durante o trabalho os motoristas referiram uso de tabaco (27%, refrigerantes à base de cola (55%, álcool (65% e café (88%, e 28% dos motoristas apresentaram mais de dez pontos na Escala de Sonolência de Epworth. Houve ainda 36% de roncadores, 5% referiram paradas respiratórias durante o sono, 12% apresentaram sensação de sufocamento, 29% sono agitado e 48% referiram sentir sono ao dirigir. Já se envolveram em acidentes de trânsito 42% dos motoristas e em 7,6% dos casos o acidente foi devido a sonolência excessiva. Aqueles com mais de dez pontos na Escala de Sonolência de Epworth apresentaram nível de atenção concentrada comprometido e quanto maior a circunferência do pescoço e a hipersonolência, menor a atenção difusa. CONCLUSÃO: No grupo de motoristas estudados, há uso alarmante de substâncias estimulantes e alta prevalência de hipersonolência diurna, que leva a diminuição da atenção.OBJECTIVE: To determine the prevalence of risk factors for obstructive sleep apnea syndrome in interstate bus drivers. METHODS: This study involved 262 professional interstate bus drivers employed by a Brazilian company headquartered in the Federal District. The drivers were evaluated using a questionnaire designed to assess respiratory sleep disorders, the Epworth Sleepiness Scale, test of sustained attention, test of divided attention and anthropometric

  17. Kajian Rumput Vetiver Sebagai Pengaman Lereng Secara Berkelanjutan

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    Susilawati Susilawati

    2016-12-01

    Full Text Available Flores is the island in the NTT province, which has a row of volcanoes, with the mountainous topography. Transportation is state road, along the coast with high cliffs on the other side. That often causes problems during the rainy season as several landslides. Various methods are used to overcome this landslide. Studies on vetiver grass as slope protection done to secure the slopes on a sustainable basis. First, an evaluation done for the slope construction along the slope safety from Nangaroro to Aegela, which are using vetiver grass as a safety slopes in addition to other security structures. It is also done for the same job of the road from Ende to Nangaroro and Ende-Detusoko. This study covers the technical aspects, ecological, construction and sustainability of the infrastructure that has been built. Furthermore, it is done the literature study to find more appropriate method, environmentally friendly and sustainable in securing these slopes problematic. From the literature studies and the field survey done, it can be concluded and recommended several models of eco-friendly structural design of vetiver grass and geotextile for slope protection, which is based on technical-meet standard strength, ecologically-friendly environment, locally-developed local wisdom, so it is easy to construct.

  18. Pelayanan Pendidikan, Medis, dan Sosial bagi Anak Cacat Secara Terpadu

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    Sunardi Sunardi

    2016-02-01

    Full Text Available This study was an effort to introduce mainstreaming in which regular schools were prepared to educate handicapped children. The study was conducted in 5 villages in Mojosongo, Boyolali. There were 19 primary schools with 4 special education teachers as itinerant teachers. It was found out that training in special education improved knowledge in special education and ability to identify handicapped children. A number of 202 handicapped children were identified (7.9% of the population. A number of 194 children received special education services in their regular classrooms. Of the 194 students, 110 of this promotion did not affect school ranks and repetition rates. Most teachers agreed that the gifted, slow learner, and learning disabled remain in the regular school with special services by special education teachers

  19. MENGELOLA KEUANGAN SECARA SYARIAH DALAM RANGKA MENUMBUHKAN GOOD MONEY HABIT

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    Aini Masruroh

    2015-10-01

    Full Text Available The wealth owned by Muslim basically is a trust from Allah that must be spent or distributed responsibly. Good habit in spending money is reflected on how the person makes a financial decision of his own. The main aspect in conducting the financial plan is the ability to save and invest. A person is qualified as having good money habits if he is able to ‚pay themselves‛ first than other interests. Meaning that each earn he have, he is able to allocate it to charity, the primary consumption, and plans for the future. Whereas the  ‚spontaneous‛ type might probably refuses to make a financial planning.DOI: 10.15408/aiq.v5i1.2557 

  20. Mengelola Keuangan Secara Syariah dalam Rangka Menumbuhkan Good Money Habit

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    Aini Masruroh

    2015-10-01

    Full Text Available The wealth owned by Muslims basically is a trust from Allah that must be spent or distributed responsibly. Good habit in spending money is reflected on how the person makes a financial decision of his own. The main aspect in conducting the financial plan is the ability to save and invest. A person is qualified as having good money habits if he is able to pay themselves first that other interest. Meaning that each earn he have, he is able to allocated it to charity, the primary consumption, and plans for the future. Whereas the spontaneous type might probably refuses to make a financial planningDOI: 10.15408/aiq.v5i1.2111

  1. Memahami Orang Lain Melalui Keterampilan Mendengar secara Empatik

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    Sukron Ma

    2013-03-01

    Full Text Available This paper discusses how to develop empathic listening skill to understand others. The purposes of writing this paper are: exploring of the concept of empathic listening and its urgency in the communication process, describing the barriers that are often experienced by a person to listen in the communication process, and describing how to develop empathic listening skills. In this paper the author uses descriptive-analytical method. The procedures are: 1 data collection, 2 classification of data, 3 data analysis, and 4 drawing conclusions. Source of the data was gained from information available in the literatures. The data analysis was done by using content analysis method (content analysis, which is an attempt to explore the symbolic meaning of the message or the contents of a book or other writings products. The results showed that one of the skills currently needed by humans and often escaped the attention is empathic listening skill. It can be said that the failure in communication may be caused by lack of adequate skills in this regard. Therefore, by developing the skill of empathic listening communication failure can be revealed, misunderstandings can be avoided and disputes can be eliminated.

  2. PUSARAN PEMILIHAN KEPALA DAERAH SECARA LANGSUNG ATAU TIDAK LANGSUNG

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    Endang Retnowati

    2015-01-01

    Full Text Available Democracy is essentially means to achieve the goal state. There are several criteria for democracy among others, the representatives of political, electoral and political accountability. Along with the development of the condition and situation of the country and nation, then democracy is also experiencing growth. There are two forms of democracy that is directly and indirectly. Based on the principles of democracy and the Republic Indonesia Constitution Year 1945, Article 24 paragraph (5, Article 56 paragraph (1, Article 40, Article 41, and Article 43 paragraph (1 of the Constitution. The decision to choose one form of local elections should be based on consideration of the element or elements of the substance, structure and culture of democracy and society, culture or democracy, the historical conditions of philosophical society, democratic education, the concept of democracy, the establishment of democracy, the form of democracy, of democracy , and evaluation of the implementation of democracy, as well as the need of attention to elements such as: goals, inputs, processes, outputs, limit, control and feedback mechanisms as well as the environment.

  3. PUSARAN PEMILIHAN KEPALA DAERAH SECARA LANGSUNG ATAU TIDAK LANGSUNG

    OpenAIRE

    Endang Retnowati

    2015-01-01

    Democracy is essentially means to achieve the goal state. There are several criteria for democracy among others, the representatives of political, electoral and political accountability. Along with the development of the condition and situation of the country and nation, then democracy is also experiencing growth. There are two forms of democracy that is directly and indirectly. Based on the principles of democracy and the Republic Indonesia Constitution Year 1945, Article 24 paragraph (5), A...

  4. Studi Gerak Kerja Pemanen Kelapa Sawit Secara Manual

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    M. Faiz Syuaib

    2015-04-01

    Full Text Available Good harvesting technique and timing is necessary to result good productivity in oil-palm industies. The harvesting activity is mostly conducted by ‘human powered’ manual handling which quite arduous and risky in term of work safety and musculoskeletal disorders (MSD. This research deals with anthropometry and motion study to analyse manual harvesting activity in oil palm plantation so the activity can be done in more safety, efficient and productive. Motion study using Natural Range of Motion (ROM is applied to know the level of motion risk based on ROM indeks and appropriate antropometry. Manual harvesting tasks by using conventional tools named ‘dodos’ and ‘egrek’ were studied in this research. The aims of this research is to know the pattern and risks distribution of the work motions, and then to determine a good harvesting procedure to minimize the risk. The anthropometry result show that the harvester posture is ideal and uniform. The ‘cutting with egrek (CuE’ was found as the most risky work element in the manual harvesting task, and the MSD risks occur on the neck, shoulder, forearm, back and ankle.Such work procedures should be designed and appropriate working distance and length of tool are required to prevent the risks. Work motion simulation revealed that 1.5, 2.5, 5.5, and 8.5 mare suitable working distance to harvest 3, 6, 12 dan 18 mheight of targeted bunches, respectively.

  5. UPAYA PENGAKARAN Echinacea purpurea L DENGAN AUKSIN SECARA KULTUR JARINGAN

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    Heru Sudrajat

    2013-03-01

    Full Text Available Recently, to meet the needs of botanicals medicinal plants the cultivation directed, require good quality and uniform seeds on form and time.    Tissue culture research to from root of Echinacea purpurea L by the provision of IBA and NAA will be conducted.This recearched aimed to develop an and cultivate qualified Echinacea purpurea L as the raw material for drug foemulation. Tissue culture techniques has  more advantage because it is not affected by the climate, relatively fast production, free contamination of microbial and do not require large tracts of land. The research was carried out considering the need to be developed and cultivated plant Echinacea purpurea L qualified as raw material for the drug tissue culture techniques has advantages because it is not affected by the climate with relatively fast production time, free of microbial contamination and do not require large tracts of land. Shoots multipication done on MS medium using growth regulators GA3with concentration of 3 mg / l. Research foor rof induction on Echinacea purpurea L shoot made by adding growth regulators of IBA and NAA with concentrations with 0, 2, 4 and 6 mg / l respectively. The 2 months incubation periods produced tehe best root induction on the additon of IBA 4 mg / l. It producted 16 roots with a length of 4 cm and relatively lagre in size.Keywords: Echinacea purpurea L., tissue culture, IBA, NAA, plant growth regulators

  6. PEMBUATAN PUPUK FOSFAT DARI BATUAN FOSFAT ALAM SECARA ACIDULASI

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    Faleh Setia Budi

    2012-02-01

    Full Text Available Phosphate rock containing P2O5 can be used as raw material of phosphate fertilizer. Phosphate rock canbe found in many areas in Indonesia, but its P2O5-content is relative low. In this research, phosphate rockfrom Rembang (P2O5-content 11.37% is used as raw material for phosphate fertilizer production byacidulation process using phosphoric acid solution. Operation variables are phosphoric acid concentration(10, 20, 30, 40 %-vol, spraying model of phosphoric acid solution (one hole and many holes, andspraying position of phosphoric acid solution (end, centre, end & centre. The result shows that theincrease of phosphoric acid concentration is proportional to P2O5-content in phosphate fertilizer withhighest P2O5-content in the amount of 18.29%. One-hole spraying model and end-position spraying giveshighest phosphate fertilizer yield in amount of 169.5 grams.

  7. PEMULIHAN GIZI BURUK SECARA RAWAT JALAN DI PUSKESMAS

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    S. Suwarti

    2012-11-01

    Full Text Available OUT-PATIENT MANAGEMENT FOR SEVERE MALNOURISHED CHILDREN IN PUSKESMASBackground: During economic crisis in Indonesia in mid 1997, the numbers of malnourished children has increased. Many efforts had been conducted to solve this problem to prevent the detrimental effect of malnutrition especially in the reduction of the quality of manpower. Since 1980's our ambulatory clinic has developed the management for malnourished children. From 1997 to 2000, cases of malnourished children increased from 27 to 193, but we were facing with the numbers of defaulters. There were 40-50% drops out patients. To solved this problem, we worked together with Districts Health Office (Dinas Kesehatan Kabupaten and Kota Bogor to build nutrition clinic in 3 health centers (Puskesmas, in South Bogor, Sukaraja, and Sukamanah sub district. Objectives: To build the nutrition clinic in health center. Methods: First step of our action was to train the medical doctors, nurses and nutritionist from the health centers in ambulatory management (registration, anthropometric measurement, nutrition counseling, clinical examination and medication for infectious diseases of severe malnourished children. The second step was to arrange the nutrition clinic in the health centers. In the first three months, the clinic was run by the team of the Nutrition Research and Development Center together with the team of health centers. In the second three of months, the health centers team run the clinic and NRDC team only supervised them. Results: The activity of nutrition clinic in the health centers was no difference with the activity in the nutrition clinic in the NRDC. The children nutritional status was improved after 4 months intervention. The improvement was measured by weight-for-height as well as by weight-for-age. The weight-for-height index was found as the perfect indicator to measure the effect of intervention on the nutritional status of the children. Conclusions: Community health centers staffs has a potential capacity to solve malnutrition problem, especially when they were trained adequately by proper trainer, and the best parameter to evaluate nutritional status was weight-for-height. Keywords: out-patient, severe malnutrition

  8. PEMBUATAN PEPTON SECARA ENZIMATIS MENGGUNAKAN BAHAN BAKU JEROAN IKAN TONGKOL

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    Tati - Nurhayati

    2014-06-01

    Full Text Available AbstrakJeroan ikan tongkol memiliki kandungan protein yang hampir sama dengan daging ikan sehingga memilikipotensi yang besar untuk dijadikan pepton. Penelitian ini bertujuan memproduksi pepton dari jeroan ikantongkol menggunakan enzim papain dan mengujinya untuk pertumbuhan bakteri. Konsentrasi enzim optimalyang digunakan untuk hidrolisis protein jeroan ikan tongkol adalah 0,26% dengan waktu hidrolisis selama3 jam. Pepton jeroan ikan tongkol memiliki kadar air 7,66%; kadar abu 2,34%; kadar protein 50,18%; dankadar lemak 0,47%. Kadar asam amino tertinggi dan terendah masing-masing adalah asam glutamat (6,38%dan sistein (0,68%. Pepton jeroan ikan tongkol memiliki kelarutan 98,20%; total nitrogen 8,03%; α-aminonitrogen bebas 1,12%; rasio asam amino bebas terhadap total nitrogen 13,93%; kadar garam 0,79%; rendemen3,92-5,54% dan pH 6,02. Pepton jeroan ikan tongkol dapat digunakan sebagai salah satu penyusun media luriabroth (LB untuk pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Kecepatan pertumbuhanspesifi k maksimum E. coli pada media yang menggunakan pepton jeroan tongkol lebih tinggi dibandingkandengan pepton komersial, sedangkan kecepatan pertumbuhan spesifi k maksimum S. aureus lebih rendah padamedia pertumbuhan LB yang mengandung pepton jeroan tongkol.Kata kunci: hidrolisis, jeroan ikan, papain, pepton

  9. PENGEMBANGAN BIAKAN IN-VITRO PLASMODIUM FALCIPARUM SECARA KONTINU

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    Sekar Tuti

    2012-09-01

    Full Text Available To support malaria research on its' serology/immunology, chemotherapy, drug sensitivity aspects etc. especially for falciparum malaria, a large amount of antigen (parasites is needed. These antigen could not be obtained from patients in the field only. Considering this situation, attempts have been made to develop a Plasmodium falciparum continuous culture   in-vitro following a method introduced  by Trager and Jensen (1976. In our laboratory, the parasite grew and multiplied nicely for 60 days. During that period of cultivation, a large amount of parasites (mostly mature trophozoite and schizont stages have been collected for antigen production. Several tubes of mostly young trophozoites stage have been preserved, it can be cultured again in the future or transported to another laboratory for further culture.

  10. Perekayasaan mesin injeksi bakalan acuan sepatu secara sederhana

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    Agustin Suraswati

    1995-06-01

    Full Text Available Engineering of the injection molding machine to mold a master last made of plastics was done in the simple mechanism. Machine specification is a single screw, screw diameter 40 mm, ratio L/D = 24/1, heater 220/380 V. 700 W, motor 5,5 Hp, capacity 500 cm3/ minute.

  11. HIDROLISIS PATI GARUT SECARA ENZIMATIS UNTUK PEMBENTUKAN SIKLODEKSTRIN

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    Erliza Noor

    2008-08-01

    Full Text Available Modified starch has important role in chemical, cosmetic, pharmaceutical, and food industries. Cyclodextrin was prepared based on garut starch using starch hidroying enzime namely 0-amylase, 0-amylase, pullulanase and glukoamylase. Cyclication to form cyclodextrin was obtained using CGTase. The highest concentration of cyclodextrin was obtained by glucoamylase and CGTase concentration of 150 unit/g substrate which was 81.11g.l-1 in 90 minutes

  12. Desain Model Diagnostik Resiko Ergonomi pada Kelapa Sawit Secara Manual

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    Nugrahaning Sani Dewi

    2015-04-01

    Full Text Available Oil palm harvesting activity is mostly done by ‘human power’ manual handling, therefore the activity may cause work safety and health problems.This research is deal with anthropometry, motion study and biomechanic to find out the risk of manual handling and to develop ergonomic assessment model of oil palm harvesting. This model was designed in the aims to find out better work motion, good procedure and better design of harvesting tool, so the manual harvesting can be done in more safe, efficient and productive. The formula for the appropriate distance between harvester’s position and the tree and the length of egrek show that oil palm harvesting for more than 16 m bunches’s height is not safe with ‘egrek’. Critical load for neck, shoulder and forearm are 21.85 N, 1091.96 N and 1634.31 N. Ergonomic risk assesment’s tool of oil palm manual harvesting was designed with the parameter: appropriate distance (dt, the length of egrek (lp, critical range of motion (CRM and critical load (CL for neck, shoulder and forearm.

  13. TEKNOLOGI PEMELIHARAAN LARVA KERAPU SUNU (Plectropomus leopardus SECARA MASSAL

    Directory of Open Access Journals (Sweden)

    Titiek Aslianti

    2016-12-01

    Seed production technology of leopard coral trout, Plectropomus leopardus by improving hatchery management had been conducted in order to increase survival rate and to produce seed continuity. The initial feeding can successfully support in larval rearing. Feed organism as rotifer, trochophore gonad, egg yolk emulsion, and artificial feed emulsion, had been used as an initial feed. The twelve of concrete tanks with 6 m3 capacity were stocked with coral trout eggs at density 100,000—150,000 eggs/tank. Artemia nauplii, artificial feed, and mysid as feed, start on larvae D20 up to juvenile stage (D45. Growth rate and survival rate were observed and calculated when the experiment was terminated. The data was analyzed by descriptive. Nutrition value of food was analyzed by proximate and fatty acid composition. The others parameters such as deformity and water quality were observed. The result showed that artificial feed emulsion and egg yolk emulsion as an initial feeding can be improve the growth rate and increase survival rate of larvae. The range of total length, body weight and survival rate of the seed i.e. 1.95—2.85 cm; 0.64—0.73 g, and 0.25%— 3.97% with the daily growth rate 3,9%—4,22%, respectively. No back bone deformity in the seed, that is 21—23 segments with interspaces of segments 0.030—0.036 mm.

  14. Pemantauan Parameter Panel Surya Berbasis Arduino secara Real Time

    Directory of Open Access Journals (Sweden)

    Muhammad Rizal Fachri

    2015-09-01

    Full Text Available Monitoring the output parameters of a solar module is required for assessing its performance under real operating conditions. This paper presents a new technique for monitoring the output parameters i.e. current and voltage of a solar module directly under real operating conditions. In this new monitoring technique, the output parameters of a solar module can be directly processed in real time condition and their results are displayed in a graph. The monitoring system is developed using microprocessor Arduino Atmega 328P and equipped with calibrated current and voltage sensors, a data acquisition system which is integrated directly into an Excel spreadsheet using the PLX-DAQ application program and a memory card for backup. The monitoring system is connected to a computer using a RS232 serial port. The collected data is saved directly into a spreadsheet and plotted in real time. This technique provides an easy access to the collected data for further analysis.

  15. Penyelesaian Sengketa/Perselisihan Secara Adat Gampong di Aceh

    Directory of Open Access Journals (Sweden)

    Taqwaddin Husin

    2015-12-01

     Customary Dispute Settlement in Aceh ABSTRACT: The existence of the village as MHA (customary villagers is pursuant to Article 18B paragraph (2 1945. The problem raised are what and how the rigths of indigenous people’s traditional form of the empirical level. Aceh’s special and special autonomy, began to fill with the implementation MHA gave the authority to the village to perform customary judicial system to resolve customary disputes. Right and authority are firmly set in the rule, Qanun Aceh Number 9, 2008, 20, 2008, 60 and the Governor Regulation Number 2013, and becomes more operational by the Joint Decree of the governor, police chief of Aceh, and the Aceh Adat Council in 2012. Thus, in formal judicial, gampong as MHA has become the basis of legal authority and strong enough.

  16. Mulheres e esporte de risco: um mergulho no universo das apneístas Women and risk sports: diving into the universe of apneístas

    Directory of Open Access Journals (Sweden)

    Luciana Silva Abdalad

    2011-06-01

    Full Text Available Este estudo, de natureza qualitativa propõe-se investigar, sob a ótica do Imaginário Social, os sentidos de limite, risco e corpo para mulheres praticantes de mergulho em apneia, no qual a vivência de uma prática de risco extremo é deliberadamente escolhida pelas informantes. Nossa amostra, intencional, foi composta por oito mulheres que praticam o esporte. O procedimento metodológico utilizado para a interpretação das falas foi a Análise do Discurso proposta por Orlandi (1987, 1993, 1999. Também trabalhamos com a interpretação das palavras que emergem na realização da técnica da Associação de Ideias (ABRIC, 1994. Nove grupos semânticos organizaram-se em torno de um grupo de associações comuns e outro de associações não comuns. As mergulhadoras das grandes profundidades sabem que ao praticarem um esporte de risco extremo desafiam seus limites corporais, e precisam tomar cuidado para não extrapolar esses limites. É preciso estendê-los. A preocupação parece estar presente na vivência deste limiar entre a vida e a morte.This study of qualitative nature aims at investigating, from a Social Imaginary perspective, the senses of limit, risk and body are for the women who practice apneic diving. The experience of this extreme risk practice is deliberately chosen by informants. Our intentionally selected sample was made up of eight women who practice the sports. The method applied for the interpretation of those speeches was Orlandi's Discourse Analysis (1987, 1993, 1999. We have worked with the interpretation of words that emerge from the Idea Association (ABRIC, 1994 technique. Nine semantic groups were organized around a group of common associations and another group of uncommon associations. The divers who go down great depths know that the practice of that sport of high risks challenges their body limits, and they must be careful not to go beyond those limits. They must extend them. That concern seems to be present in the experience of that boundary between life and death.

  17. Apn'l2O06 ' EAST AFRICAN MEDICAL JOURNAL ' 105

    African Journals Online (AJOL)

    2006-04-04

    Apr 4, 2006 ... alternative automated techniques for the determination of ... and manual techniques have been developed. These ... a Becton Dickson's automated instrument is designed ..... applications for developing countries, Washington,.

  18. Putative hAPN receptor binding sites in SARS_CoV spike protein

    Institute of Scientific and Technical Information of China (English)

    YUXiao-Jing; LUOCheng; LinJian-Cheng; HAOPei; HEYou-Yu; GUOZong-Ming; QINLei; SUJiong; LIUBo-Shu; HUANGYin; NANPeng; LIChuan-Song; XIONGBin; LUOXiao-Min; ZHAOGuo-Ping; PEIGang; CHENKai-Xian; SHENXu; SHENJian-Hua; ZOUJian-Ping; HEWei-Zhong; SHITie-Liu; ZHONGYang; JIANGHua-Liang; LIYi-Xue

    2003-01-01

    AIM:To obtain the information of ligand-receptor binding between thd S protein of SARS_CoV and CD13, identify the possible interacting domains or motifs related to binding sites, and provide clues for studying the functions of SARS proteins and designing anti-SARS drugs and vaccines. METHODS: On the basis of comparative genomics, the homology search, phylogenetic analyses, and multi-sequence alignment were used to predict CD13 related interacting domains and binding sites sites in the S protein of SARS_CoV. Molecular modeling and docking simulation methods were employed to address the interaction feature between CD13 and S protein of SARS_CoV in validating the bioinformatics predictions. RESULTS:Possible binding sites in the SARS_CoV S protein to CD13 have been mapped out by using bioinformatics analysis tools. The binding for one protein-protein interaction pair (D757-R761 motif of the SARS_CoV S protein to P585-A653 domain of CD13) has been simulated by molecular modeling and docking simulation methods. CONCLUSION:CD13 may be a possible receptor of the SARS_CoV S protein which may be associated with the SARS infection. This study also provides a possible strategy for mapping the possible binding receptors of the proteins in a genome.

  19. PENGARUH KONDISI ATMOSFERIK TERHADAP PERHITUNGAN PROBABILISTIK DAMPAK RADIOLOGI KECELAKAAN PWR 1000-MWe

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    Pande Made Udiyani

    2015-10-01

    Full Text Available ABSTRAK PENGARUH KONDISI ATMOSFERIK TERHADAP PERHITUNGAN PROBABILISTIK DAMPAK RADIOLOGI KECELAKAAN PWR 1000-MWe.  Perhitungan dampak kecelakaan radiologi terhadap lepasan produk fisi akibat kecelakaan potensial yang mungkin terjadi di Pressurized Water Reactor (PWR diperlukan secara probabilistik. Mengingat kondisi atmosfer sangat berperan terhadap dispersi radionuklida di lingkungan, dalam penelitian ini akan dianalisis pengaruh kondisi atmosferik terhadap perhitungan probabilistik dari konsekuensi kecelakaan reaktor.  Tujuan penelitian adalah melakukan analisis terhadap pengaruh kondisi atmosfer berdasarkan model data input meteorologi terhadap dampak radiologi kecelakaan PWR 1000-MWe yang disimulasikan pada tapak yang mempunyai kondisi meteorologi yang berbeda. Simulasi menggunakan program PC-Cosyma dengan moda perhitungan probabilistik, dengan data input meteorologi yang dieksekusi secara cyclic dan stratified, dan disimulasikan di Tapak Semenanjung Muria dan Pesisir Serang. Data meteorologi diambil setiap jam untuk jangka waktu satu tahun. Hasil perhitungan menunjukkan bahwa frekuensi kumulatif  untuk model input yang sama untuk Tapak pesisir Serang lebih tinggi dibandingkan dengan Semenanjung Muria. Untuk tapak yang sama, frekuensi kumulatif model input cyclic lebih tinggi dibandingkan model stratified. Model cyclic memberikan keleluasan dalam menentukan tingkat ketelitian perhitungan dan tidak membutuhkan data acuan dibandingkan dengan model stratified. Penggunaan model cyclic dan stratified melibatkan jumlah data yang besar dan pengulangan perhitungan  akan meningkatkan  ketelitian nilai-nilai statistika perhitungan. Kata kunci: dampak kecelakaan, PWR 1000-MWe,  probabilistik,  atmosferik, PC-Cosyma   ABSTRACT THE INFLUENCE OF ATMOSPHERIC CONDITIONS TO PROBABILISTIC CALCULATION OF IMPACT OF RADIOLOGY ACCIDENT ON PWR-1000MWe. The calculation of the radiological impact of the fission products releases due to potential accidents

  20. Penetapan Kadar Allopurinol Dalam Omeric Tablet Produksi PT. Mutifa Medan Secara Spektrofotometri Ultraviolet

    OpenAIRE

    Martina Rotua MS

    2011-01-01

    Obat adalah semua bahan tunggal atau campuran yang digunakan oleh semua makhluk untuk bagian dalam maupun bagian luar, guna mencegah, meringankan, maupun menyembuhkan penyakit. Menurut undang-undang kesehatan, yang dimaksud dengan obat adalah suatu bahan atau campuran bahan yang dimaksudkan untuk digunakan dalam menentukan diagnosis, mencegah, mengurangi, menghilangkan, menyembuhkan penyakit atau gejala penyakit, luka atau kelainan badaniah atau rohaniah pada manusia atau hewan, termasuk memp...

  1. KEEFEKTIFAN EKSTRAK MENGKUDU PADA BERBAGAI KONSENTRASI TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI RALSTONIA SP. SECARA IN VITRO

    Directory of Open Access Journals (Sweden)

    Efri dan Titik Nur Aeny .

    2012-02-01

    Full Text Available Effectivity of  Morinda citrifolia extract on in-vitro growth inhibition of Ralstonia sp.  Morinda citrifolia has been known as one of plants having numerous medicinal properties.  Extract of the fruits shows antibacterial activity against several bacteria including Pseudomonas aeruginosa.  The purpose of this study was to observe inhibitor effect of  M. citrifolia fruit extract, and to investigate the effect of different concentrations of  M. citrifolia fruit extract on in-vitro growth of  Ralstonia (Pseudomonas sp., the causal agent of banana bacterial wilt disease in Lampung.  Inhibition effect of  M. citrifolia was determined by the presence of inhibition zone, and concentration effect was determined by the diameter of  inhibition zone . The study consisted of two experiments.  The treatments of the first experiment were three different kinds of fruit extract, namely the whole fruit, the seeds, and the fleshy parts, and the second one consisted of six different levels of extract concentrations.  The results showed that the whole fruit extract was the most effective as an in-vitro growth inhibitor, and the higher the concentration level the higher the growth inhibition zone formed.

  2. Penetapan Kadar Dextromethorphan HBr Dalam Sediaan Sirup Dextromethorphan Secara Kromatografi Cair Kinerja Tinggi

    OpenAIRE

    2011-01-01

    Semenjak dunia berkembang dan dihuni oleh manusia serta mahluk hidup lainnya, mungkin sudah ada penyakit dan usaha untuk mengobatinya. Keadaan sehat dan sakit adalah dua hal yang tidak dapat dipisahkan, ini berlaku bagi semua mahluk hidup. Salah satu kebiasaan manusia yang diwarisi dari nenek moyangnya, ialah melakukan pengobatan sendiri jika menderita sakit. Pengobatan sendiri di Indonesia dilakukan dengan menggunakan obat tradisional atau jamu, dan obat-obatan yang dijual bebas baik dari go...

  3. Perkiraan Kestabilan Tegangan Secara Dinamis Menggunakan Eksponen Lyapunov Maksimum (Studi Kasus Sistem Kelistrikan Wilayah Lampung

    Directory of Open Access Journals (Sweden)

    M. Widi Triyatno

    2015-03-01

    Full Text Available Disturbances in the operation of the power system may cause disturbance in voltage stability. Therefore, dynamic voltage stability analysis before and after disturbance needs to be performed. This paper proposes dynamic voltage stability prediction using maximum Lyapunov exponent with Lampung’s electrical system as case study. Voltage stability simulation is performed with various types of disturbances that occur at line between of Baturaja substation and Bukit Kemuning substation. Time-series data of voltage measurement of simulation results at GI Baturaja is applied for voltage stability prediction analysis using maximum Lyapunov exponent. With the same number of data samples and the same time for circuit breakers to interrupt disturbances, the simulation results using maximum Lyapunov exponent show that the voltage can be stabilized at 1.7 seconds after the occurrence of the three-phase disturbance, at 1.2 seconds after the occurrence of the phase-to-ground disturbance, at 0,9 second after the occurrence of the disturbance between phase, at 1.2 seconds after the occurrence of the loss of line disturbance and 1.4 seconds after the occurrence of the loss of load disturbance. The amount of data samples used in analysis affect the time for the voltage reaches stability.

  4. Sistem Pendukung Keputusan Penentuan Lokasi dan Pemetaan Secara Simultan dengan OLAP

    OpenAIRE

    Harindra W Pradhana; Suryono Suryono; Achmad Widodo

    2016-01-01

    This research proposes simultaneous localization and mapping (SLAM) system processed separately from the robot using data ware house. Information in the data warehouse processed separately to allow complex computation held without lowering robotic system performance. The system helps making decision by analyzing information in the data warehouse. Decision support information visualized in vector graphics to helps robotic information interpretation. This system use online analytical processing...

  5. EFISIENSI CONSTRUCTION WETLAND TYPHA SP. SEBAGAI PENGOLAH AIR LIMPASAN JALAN RAYA SECARA ALAMI

    Directory of Open Access Journals (Sweden)

    Rudatin Windraswara

    2011-02-01

    Full Text Available Penelitian ini bersifat desain eksperimental pada skala laboratorium untuk mengetahui kemampuan Typha latifolia. sebagai tanaman wetland dalam mereduksi BOD dan COD yang berasal polutan air limpasan jalan raya sebagai bagian dari sistem drainase yang berkelanjutan. Sampel dalam penelitian ini adalah air limpasan jalan yang berasal dari air hujan yang kemudian masuk ke saluran pengumpul (drainase jalan. Habitat wetland disimulasikan menggunakan kolom dengan tabung yang memiliki volume kosong 20 liter. Susunan tabung adalah sebagai berikut; tanaman wetland, air, pasir dan batu kerikil. Satu tabung lagi akan berlaku sebagai kontrol dengan susunan yang sama tanpa tanaman wetland. Desain tabung memiliki spesifikasi sebagai berikut bahan acrylic dengan ukuran diameter 9,7 cm, tinggi 40 cm, volume 20 liter, media pasir setinggi 10 cm, kerikil 15 cm, kemudian diisi air setinggi 15 cm dari batas kerikil. Nilai BOD dari sampel kontrol setelah hari ke-3 menjadi 87 mg/l dari nilai semula 104 mg/l sedangkan nilai BOD dari sampel uji setelah hari ke-3 menjadi 44 mg/l dari nilai semula 104 mg/l. Hasil ini menunjukkan tanaman tersebut mampu menghilangkan nilai BOD sebesar 65% atau BOD removal sebesar 65%. Nilai COD dari sampel kontrol setelah hari ke-3 menjadi 309 mg/l dari nilai semula 210 mg/l sedangkan nilai COD dari sampel uji setelah hari ke-3 menjadi 87 mg/l dari nilai semula 210 mg/l. Hasil ini menunjukkan tanaman Typha latifolia mampu menghilangkan nilai COD sebesar 58,6% atau COD removal sebesar 58,6%. Kedua parameter tersebut telah sesuai dengan baku mutu Kepmen LH no 112 tahun 2003 dan Kepmen LH no 51 tahun 1999.

  6. RESEPSI KESADARAN BERBAHASA SECARA KRITIS DALAM AKTIVITAS PENULISAN FIKSI GURU DAN SISWA

    Directory of Open Access Journals (Sweden)

    Suminto Sayuti

    2013-01-01

    Full Text Available This study aims to describe the reception of critical language awareness (CLA in the fiction writing process among senior high school (SHS teachers and students. It was a content analysis supported by qualitative data collected through focus group discussions. The subjects were SHS teachers and students in Yogyakarta Special Territory. The data analysis in the process of interpreting meanings of their works was based on the CLA elements. The findings showed that their reception of CLA in fiction writing was reflected in both the process and the outcome. The teachers were still not optimal enough in developing methods, using media, and selecting the assessment system in the learning of fiction writing. Most of the students’ works did not reveal the awareness of the importance of language power and self-position as writers.

  7. Pengembangan Model Economic Production Quantity (EPQ dengan Sinkronisasi Demand Kontinu dan Diskrit Secara Simultan

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    Nurike Oktavia

    2016-04-01

    Full Text Available The most popular inventory model to determine production lot size is Economic Production Quantity (EPQ. It shows enterprise how to minimize total production cost by reducing inventory cost. But, three main parameters in EPQ which are demand, machine set up cost, and holding cost, are not suitable to solve issues nowadays. When enterprise has two types of demand, continue and discrete demand, the basic EPQ would be no longer useful. Demand continue comes from customer who wants their needs to be fullfilled everytime per unit time, while fullfillment of demand descrete is at a fixed interval of time. Literature review is done by writers to observe other formulation of EPQ model. As there is no other research can be found which adopt this topic, this study try to develop EPQ model considering two types of demand simoultaneously.

  8. Analisis Sistem Proses Pindah Massa pada Ekstraksi secara Mekanik Minyak Kedelai (Glycine Max Oil

    Directory of Open Access Journals (Sweden)

    Bambang Dwi Argo

    2010-10-01

    Full Text Available Soybean, a vegetable protein-rich commodity needed to improve public nutrition, safe to consume, and the price affordable. The purpose of this research are to: 1. determine the effect of pressure and time of extraction the mass balance, yield and level of soybean oil extraction, 2. Gain mass transfer coefficient value in soybean oil extraction process, and 3. analyze the mechanism of mass changes during the extraction process soybean oil is mechanically using hydraulic pressing. Oil obtained results indicate an increase due to the influence of a given amount of pressure and length of time silenced. The greater the pressure exerted, the more oil produced this shows soybean oil can come out with a maximum at the greatest pressure of 200 kg/cm2. Mass transfer coefficient in soybean oil extraction process at a pressure of 100 kg/cm2 5.57x10-5 gcm3/cm seconds. In the pressure is 200 kg/cm2 15.39 x 10-5g cm3/cm seconds.

  9. Sistem Pendukung Keputusan Penentuan Lokasi dan Pemetaan Secara Simultan dengan OLAP

    Directory of Open Access Journals (Sweden)

    Harindra W Pradhana

    2016-01-01

    Full Text Available This research proposes simultaneous localization and mapping (SLAM system processed separately from the robot using data ware house. Information in the data warehouse processed separately to allow complex computation held without lowering robotic system performance. The system helps making decision by analyzing information in the data warehouse. Decision support information visualized in vector graphics to helps robotic information interpretation. This system use online analytical processing (OLAP on processing and analyzing information in the data warehouse. Visualization process use SVG to display map from robotic data processing. Web server manages all of the processing and analysis of OLAP information and displays it through browser to the user. This research produces interactive and informative decision support system. The web server quickly process information and displays it to the browser. System processing and analysis generates relevant information with low error. For further research, conventional SLAM methods can be added to the server to operate the information in the data warehouse.

  10. Multiplikasi, Induksi Planlet dan Seleksi Tembakau Hasil Transformasi Gen Coat Protein SMV Secara Kultur in Vitro

    Directory of Open Access Journals (Sweden)

    N.A. FITRIYAH

    2004-11-01

    Full Text Available A study has been conducted to screen and multiply calluses and buds oftobacco transformed with the Soybean Mosaic Virus (SMV coat protein gene and to induce them to plantlet formation by using MS medium with phytohormon treatments. The multiplication of calluses and buds was carried out by sub-culturing on MS medium using 0.3 mg/L NAA+1 mg/L BAP, followed by induction of plantlet formation on MS medium supplemented with NAA and BAP at varied concentration. The results showed that tobacco calluses transformed with SMV coat protein gene was multipliable on MS medium supplemented with NAA at a concentration of 0.3 mg/mL and BAP at 1 mg/L concentration. In this experiment kanamycin was used at a concentration of 100 μg/mL which resulted in the viability level of 84%. On MS medium supplemented with 0.3 mg/L NAA, 0.1 mg/L BAP, and 100 μg/mL kanamycin, induction of calluses to plantlet formation reached 20% level.

  11. Uji Validasi Pada Estimasi Kandungan Kafein Dan Asam Sitrat Dalam Minuman Energi Secara Spektrofotometri Derivatif

    OpenAIRE

    Dalimunthe, F. Dadang

    2015-01-01

    According to SNI 01-6684-2002 Energy drinks are beverages that contain one or more ingredients are easily and quickly absorbed into body to produce energy with or without food additives are permitted. Derivative spectrophotometry is a transformation method from conventional spectrophotometric that developed for quantitative analysis of multicomponent active compounds in a preparation. The aim of this study was to test the validation of derivative spectrophotometry method in estimating the co...

  12. PERENCANAAN PEMBANGUNAN GUGUS PULAU SAPEKEN SECARA BERKELANJUTAN: PENILAIAN DAYA DUKUNG KAWASAN BAGI PENGEMBANGAN WISATA

    Directory of Open Access Journals (Sweden)

    A Romadhon

    2013-08-01

    Full Text Available The challenge for planning in small island is to ensure efficient use of limited land resources, ensure balanced regional development and balanced use of resources, including natural resources and landscape resources. Based on carrying capacity and small island destination, spatial planning of tourism in Sapeken archipelago which contaian several small island, done by suitability analyze Sapeken archipelago for several tourism. Furthermore,carrying capacity calculated referring Yuliandaetal(2010 to assess how much tourism can commodate without causing disruption the nature. The result suggest Sapeken archipelago have suitable area for dive tourism (164.42 ha; snorkeling (361.56 ha; fishing tourism (1493.38 ha, mangrove tourism (3927.09 ha dan beach tourism (39.77 ha. Carrying capacity for tourism activities,Sapeken archipelago can commodate 4260 person/day for dive tourism; 3590person/dayfor snorkelling; 14 900people/day for fishing tourism; 321. 000people/day formangrovetoursm;and 199 people/day for beach tourism.

  13. Penetapan Kadar Natrium dan Kalium Dalam Biji Salagundi (Vitex trifolia L.) Secara Spektrofotometri Serapan At

    OpenAIRE

    Siregar, Nugraha

    2016-01-01

    Salagundi (Vitex trifolia L.) is clump and wild plant which including in Verbeneaceae tribe. Traditionally, Salagundi seed is used as antihypertensive medicine by society of Kabanjahe, North Sumatera. A research in China explained that seed of salagundi has antihypertension effects, but it doesn’t explained that antihypertension effects come from organic or anorganic content in seed of salagundi. Antihypertension effects is estimated derive from anorganic contents, that is sodium and potassiu...

  14. Daya Antibakteri dan Waktu Kontak Infusa Teh Hijau (Camellia sinensis Terhadap Salmonella typhi Secara In Vitro

    Directory of Open Access Journals (Sweden)

    Dione Margareth Setiawan

    2010-06-01

    Full Text Available Green tea (Camellia sinensis contains cathecin which has been reported to have various pharmacologic properties, such as an antibacterial agent. Salmonella typhi, as agent of typhoid fever, remains a public health problem in tropical countries; about 20 million cases and 600.000 deaths annually all over the world. Objectives of this research were to observe the antibacterial activities and contact time of green tea infusion againsts Salmonella typhi by in vitro experiment. The experiment took place in Microbiology Laboratory, School of Medicine, Padjadjaran University, Bandung, March-April 2009. Methods: In vitro laboratory analytic study has been conducted on green tea infusion of Indonesian and Japanese commercial package againsts Salmonella typhi. The study used agar well diffusion method and analyzed by ANAVA and t-independent test. Results: Only at concentration of 40% (w/v, Indonesian green tea infusion gave an average inhibition area of 3.376±0.334 mm diameter, and 3.571±0.217 mm on Japanese package, while below 40% were 0.707±0.000 mm with no differences between both packages (p>0.551. There has been observed any turbidity in all Muller Hinton liquid media on both packages compared with control medium, also any growth of Salmonella typhi collony in all Muller Hinton agar at concentrations below 40%. Green tea infussion on both packages has been observed to have antibacterial activities at 40% but neither been observed at concentration below 40%.

  15. Pengolahan Sampah Secara Pirolisis dengan Variasi Rasio Komposisi Sampah dan Jenis Plastik

    Directory of Open Access Journals (Sweden)

    Qonita Rachmawati

    2015-03-01

    Full Text Available Pada tahun 2012, sampah yang dihasilkan sebesar 1200 ton/hari. Jika permasalahan sampah di Kota Surabaya tidak ditangani dengan baik maka akan menimbulkan beberapa masalah antara lain: masalah kesehatan dan masalah kebersihan. Oleh karena itu, diperlukan metode yang dapat mengolah sampah namun tidak menimbulkan masalah baru lainnya. Salah satu metode pengolahan sampah yang telah dikembangkan, yaitu metode pirolisis. Penelitian ini bertujuan untuk menentukan pengaruh jenis plastik dan komposisi terhadap produk hasil pirolisis. Pada penelitian ini digunakan reaktor dengan kapasitas 500 g yang berbahan stainless steel. Variabel yang digunakan yaitu jenis sampah plastik dan komposisi sampah. Jenis sampah yang digunakan yaitu sampah plastik HDPE (High Density Polyethylene, PET (Poly Ethylene Terephthalate, dan PS (Poly Styrene. Komposisi sampah yang digunakan antara lain: 100:0, 75:25, dan 50:50. Temperatur yang digunakan pada reaktor yaitu 500°C dengan waktu 30 menit. Penelitian dimulai dari persiapan bahan uji, persiapan reaktor, percobaan pendahuluan, dan penelitian dengan reaktor pirolisis. Selanjutnya dilakukan analisis untuk masing-masing hasil produk. Penelitian ini jenis sampah plastik yang menghasilkan gas tertinggi yaitu jenis plastik PET sebesar 45,40% dan jenis plastik yang menghasilkan wax tertinggi yaitu jenis plastik HDPE sebesar 69,91%. Sedangkan komposisi yang menghasilkan gas tertinggi yaitu komposisi dengan ranting 25% dan PET 75% sebesar 71,24% dan komposisi yang menghasilkan wax tertinggi yaitu komposisi dengan ranting 25% dan PS 75% sebesar 61,36%.

  16. Kompatibilitas Jamur Entomopatogenik Paecilomyces fumosoroseus dengan Beberapa Bahan Aktif Pestisida Secara In Vitro

    Directory of Open Access Journals (Sweden)

    Fani Fauziah

    2016-12-01

    Full Text Available Untuk mengurangi penggunaan pestisida di perkebunan teh, maka salah satu upaya pengendalian hama yang dapat dilakukan adalah mengkombinasikan aplikasi pestisida dengan jamur entomopatogenik. Penelitian ini bertujuan untuk mengetahui kompatibilitas antara jamur P. fumosoroseus  dengan beberapa bahan aktif pestisida di laboratorium. Perlakuan dirancang dengan Rancangan Acak Lengkap (RAL terdiri dari 5 jenis bahan aktif yaitu metomil, bifentrin, imidakloprid, tembaga oksida dan metidation pada taraf konsentrasi sesuai rekomendasi lapang (RL, 0,5x RL dan 2x RL. Hasil pengamatan menunjukkan bahwa diantara kelima jenis bahan aktif pestisida yang diuji, metidation memiliki tingkat toksisitas yang paling tinggi. Persentase produksi spora tertinggi sebesar 13,77% ditunjukkan oleh perlakuan bifentrin 2x RL. Combining application of pesticides and entomopathogenic fungi is an alternative way for controlling pest in order to reduce pesticides application in the tea field. This research was aimed toexamine the compatibility of Paecilomyces fumosoroseus tosome active agents of pesticide in laboratory. Completely Randomized Block Designed (CRBD with 5 active agents: methomyl, bifenthrin, imidacloprid, copper oxide and metidation in three different concentration was employed in this study, which was based on the existing field recommendation (FR: 0,5x FR and 2x FR. The results showed that among the five tested pesticides, metidation appeared to be the most toxic agentto P. fumosoroseus. Meanwhile, the highest percentage of spore production was counted in bifenthrin 2x RL (13,77%.

  17. PERANCANGAN MODEL PENGUKURAN KINERJA LEAN DAN GREEN RANTAI PASOK SEMEN SECARA TERINTEGRASI

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    Dina Rahmayanti

    2017-06-01

    Full Text Available PT. Semen Padang is one of the reputable cement companies in Indonesia which is producing types of cement. Implementation of supply chain concept in PT. Semen Padang is very important to satisfy customers and stakeholders requirement. Performance measurement is a process to evaluate the supply chain effectiveness and efficiency in lean and green aspect. The problem is how to design performance measurement model which is integrating lean and green supply chain concepts. The objective of research is integrating lean and green supply chain concepts to design supply chain performance measurement of bag cement type. Integration of lean and green concept is designed by Balanced Scorecard (BSC perspectives that consist of financial, customer, internal process business and learning and growth. Key performance indicators (KPI are formulated and classified based on BSC perspectives. Next stage is pair wise comparison process to the weight value. Evaluation of KPI pair wise comparison based is conducted on expert opinion which is selected by consideration of their relevance knowledge and experience. The process of pair wise comparison is calculated to determine priority of the KPIs based on weight value by using Analytical Hierarchy Process (AHP method. The weight of KPI will be arrange by descending to determine the priority. Model of performance measurement is validated by face validity method. Research result is obtaining 25 KPIs that derived from integration of lean and green supply chain concept of bag cement type. Grouping is consisting of 3 KPIs of financial, 4 KPIs of customer, 11 KPI s of internal process business and 7 KPI s of learning & growth. Result is showing that customer is most prioritized element in supply chain of bag cement type. Implementation of proposing model is coordination of departments and employee in context of information accessibility.     Keywords: Supply chain, lean, green, BSC, performance measurement

  18. Perancangan Model Pengukuran Kinerja Lean dan Green Rantai Pasok Semen Secara Terintegrasi

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    Dina Rahmayanti

    2017-05-01

    Full Text Available Abstract PT. Semen Padang is one of the reputable cement companies in Indonesia which is producing types of cement. Implementation of supply chain concept in PT. Semen Padang is very important to satisfy customers and stakeholders requirement. Performance measurement is a process to evaluate the supply chain effectiveness and efficiency in lean and green aspect. The problem is how to design performance measurement model which is integrating lean and green supply chain concepts. The objective of research is integrating lean and green supply chain concepts to design supply chain performance measurement of bag cement type. Integration of lean and green concept is designed by Balanced Scorecard (BSC perspectives that consist of financial, customer, internal process business and learning and growth. Key performance indicators (KPI are formulated and classified based on BSC perspectives. Next stage is pair wise comparison process to the weight value. Evaluation of KPI pair wise comparison based is conducted on expert opinion which is selected by consideration of their relevance knowledge and experience. The process of pair wise comparison is calculated to determine priority of the KPIs based on weight value by using Analytical Hierarchy Process (AHP method. The weight of KPI will be arrange by descending to determine the priority. Model of performance measurement is validated by face validity method. Research result is obtaining 25 KPIs that derived from integration of lean and green supply chain concept of bag cement type. Grouping is consisting of 3 KPIs of financial, 4 KPIs of customer, 11 KPI s of internal process business and 7 KPI s of learning & growth. Result is showing that customer is most prioritized element in supply chain of bag cement type. Implementation of proposing model is coordination of departments and employee in context of information accessibility. Keywords: Supply chain, lean, green, BSC, performance measurement

  19. MUTU GIZI DAN KEAMANAN BEKASAM PRODUK FERMENTASI IKAN TERI SECARA SPONTAN DAN PENAMBAHAN KULTUR MURNI

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    Uken SS Soetrisno

    2012-11-01

    Full Text Available ABSTRACTBackground: Many kind of foods based on fermentation products are used as traditional foods in Indonesia. Such products are tape the sweet fermented cassava as source of carbohydrates, tempe the delicious fermented soybean or other beans as source of  protein, dadih the sour fermented buffalo milk as source of calcium and protein, and many others made of  animal protein source such as from small fish. Fermentation of foods will increase the nutrient content and absorbability of the nutrient, besides will increase sensoric or acceptability value of the products. Objectives: The study objectives are to evaluate the nutrient quality and sensoric quality of  the salt-fermentation product of small fish, so called teri, both using spontaneous fermentation and selected culture-fermentation those producing lactic acid bacteria. Methods: This was a food experimental study to explore the quality improvement  of acid bacteria fermentation products using spontaneous or sellected culture on protein source foods. Ingredients were consist of small fish with steam rice at 1 to 1 by weight with addition of salt at 5-6% and fermented spontaneously or with addition of 1% level selected culture, which contain lactic acid producing bacteria. Fermentation were done for 4 to 5 days, depent on the type of culture. Results: Bekasam produced from both type of fermentation had lower protein content due to the addition of rice as carbohydrate source, while ash content became higher due to the addition of NaCl salt. The products had sweet smell besides sour taste due to carbohydrate fermentation which could increase the absorbability of the products especially vitamins and minerals those needed lower pH. Generally, fermented bekasam had higher vitamin content and changed fatty acid composition. Vitamin B12 increased by 2 folds and vitamin A by 3-4 folds, compared with those of fresh small fish. Linoleic acid increased by 2-4 folds followed by decreased of other long chain fatty acids content. Pathogenic microorganisms were still high in raw fermented products, but they were  undetected after steaming for 30 minutes. Conclusions: Nutrient contents of the fermentation products, such as vitamin A, B12, linoleic acid were higher than the fresh fish, while the proximate nutrient content depent on the amount of ingredients added as the base component. All fermentation products contained E. coli, Koliform, and Shigella s., these might come from the fish that had been polutted since in the ocean. After steaming or sterillization the product, those pathogen microorganisms were negative or undetected. Based on the nutrient quality, it will be worthwile to develop local food formula using bekasam the fermented teri, for children under five years old. [Penel Gizi Makan 2005,28(1: 31-35] Keywords: fermentation, spontanious, sellected culture, small fish, bekasam.

  20. REGENERASI RUMPUT LAUT Kappaphycus alvarezii HASIL TRANSFORMASI GEN Sitrat Sintase MENGGUNAKAN Agrobacterium tumefaciens SECARA IN VITRO

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    Emma Suryati

    2016-02-01

    seleksi 85%, dan efisiensi regenerasi thalus non transgenik sebesar 95% pada media non selektif. Media recovery dengan penambahan pupuk PES memperlihatkan sintasan yang paling baik pada regenerasi thalus transgenik. Hasil analisis PCR memperlihatkan K. alvarezii transgenik putatif mengandung transgen PaCS di bawah kendali promoter 35S CaMV.

  1. Pengembangan The Two-Tier Diagnostic Tes pada Bidang Biologi secara Terkomputersisasi

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    Suwarto Suwarto

    2013-01-01

    Full Text Available Abstrak Penelitian bertujuan mengembangkan tes diagnostik dua tingkat (the two-tier diagnostic test pada bidang biologi dan mengembangkan program komputer untuk mendiagnosis kesulitan belajar siswa. Tes disusun melalui tiga fase, yaitu (1 defining the content boundaries; (2 identifying students’ misconceptions; dan (3 developing instrument. Pengembangan program komputer terkait dengan soal, siswa, tabel kerja, SQL (Structured Query Language, ketuntasan, dan grade. Tes diagnostik yang dikembangkan ada 3, yaitu tes A, tes B, dan tes C. Responden untuk tes A dan tes B ada 130 siswa dan untuk tes C ada 128 siswa. Analisis statistik dilakukan dengan program iteman versi 3,00. Program komputer yang dikembangkan diujicobakan di SMAN 1 Tawangsari dan SMA Veteran 1 Sukoharjo. Hasil penelitian adalah Tes A terdiri atas 73 butir, dengan rentang kesulitan butir 0,031-0,962, rentang daya beda butir 0,013-1,000 dan reliabilitas 0,871; Tes B terdiri atas 39 butir, dengan rentang kesulitan butir 0,038-0,762, rentang daya beda butir 0,009-0,918 dan reliabilitas 0,768; dan Tes C terdiri atas 79 butir, dengan rentang kesulitan butir 0,102-0,938, daya beda butir 0,029-1,000 dan reliabilitas 0,894. Program komputer untuk mendiagnosis kesulitan belajar telah berfungsi dengan baik. Kata kunci: tes terkomputerisasi

  2. Meningkatkan Kualitas Pembelajaran Pada Mata Kuliah Pengantar Probabilitas Melalui Lesson Study Dengan Pengajaran Berbalik Secara Team

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    Arief Agoestanto

    2012-06-01

    Full Text Available The quality of introduction to probability lectures that were held haven’t maximized yet. It is indicated by results of student learning that only 20% got A value, the lack of interaction between students and lectures, students with students, and students with lecture. One of the efforts to improve the quality of learning is through lesson study with team reciprocal teaching. The problem in this research are as follows: (1 is the lecturers’ professionalism of Statistics Expertise Group can be improved by Lesson Study approach in the introduction to probability lectures, and (2 is interaction between students and students, students with lecture note, and students with lecturers of introduction to probability lesson can be improved by apply lesson study method with team reciprocal teaching. This research use research model based on lesson study by three steps. They are plan, do, and see which are done in three cycles. The research was conducted on odd semester in academic year 2010/2011 for 6 months in Mathematics department, Semarang State University. The results of research are (1 the increasing of lecturers’ professionalism of Statistics Expertise Group for Introduction to Probability lectures, and (2 increase student interaction with students, students with lecture note, and students with lecturers of Introduction to Probability lectures.

  3. KORELASI PADAT TEBAR DAN DEBIT AIR DALAM TEKNIK PENDEDERAN BENIH UDANG GALAH (Macrobrachium rosenbergii SECARA INTENSIF

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    Wartono Hadie

    2008-12-01

    Full Text Available Riset ini dilaksanakan untuk mengetahui korelasi antara padat tebar dan debit air dalam teknik pendederan udang galah. Riset dilakukan dengan menggunakan bak ukuran 4 m x 2 m x 0,75 m yang mempunyai sistem air mengalir. Perlakuan yang diaplikasikan adalah padat tebar dalam tiga tingkatan yaitu 250, 500, dan 750 ekor/m2 yang dikombinasikan dengan tiga tingkat debit air yaitu 0,010; 0,020; dan 0,030 liter/detik/m2. Setiap perlakuan dilakukan dengan 3 ulangan. Udang galah dalam penelitian ini adalah PL-44 dengan ukuran 0,04 g. Pendederan udang galah dilaksanakan selama 40 hari. Hasil riset menunjukkan bahwa terdapat interaksi antara variabel padat tebar dan debit air. Variabel debit air memberikan kontribusi sebesar 57% dan variabel padat tebar mempunyai kontribusi 18% dalam mendukung sintasan benih udang galah. Hasil terbaik dicapai oleh perlakuan padat tebar 500 ekor/m2 dan debit air 0,030 liter/detik/m2 dengan laju pertumbuhan harian udang mencapai rata-rata 2,84% dan sintasan sebesar 89,6% selama 40 hari masa pemeliharaan. The research aim was to evaluate the correlation between fry density and water flow rate in the nursery of giant prawn. The experiment was conducted in concrete tanks. The treatment consisted of three levels, 250, 500, and 750 fry/m2 and combination of three levels of water flow, i.e. 0.010, 0.020, and 0.030 litre/second/m2. Three replications were used in each treatments. The prawn fry were PL-14 with 0.04 g of average weight. The research was conducted for 40 days of post-larvae rearing. Result of this experiment showed that there was interaction between density and water flow. The data analysis indicated that there were 57% contribution of water flow and 18% of fry density to survival rate of giant prawn. The best result was showed by the density of 500 fry/m2 and water flow of 0.030 litre/second/m2. The specimen have specific growth rate of 2.84% and survival rate of 89.6% for 40 days of rearing.

  4. Penetapan Kadar Triklosan Pada Pasta Gigi Secara Kromatografi Cair Kinerja Tinggi (KCKT)

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    Lubis, Muhammad Fauzan

    2016-01-01

    Toothpaste is one of the important requirements for the maintenance and health of teeth and gums. Public awareness of the importance of dental hygiene make toothpaste manufactures often put out a new kind of toothpaste. People use toothpaste with the aim to eliminate germs and bacteria in the mouths so, oral health and freshness can be maintained. However, the number of dead bacteria due to the use of triclosan toothpaste use as an antiseptic it will make the chances of other bacteria grow ou...

  5. PEMBUATAN KALSIUM KARBONAT DARI BITTERN DAN GAS KARBON DIOKSIDA SECARA KONTINYU

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    Soemargono Soemargono

    2012-01-01

    Full Text Available Kalsium karbonat  yang  digunakan  dalam  industri- industri cat, karet, dan  kertas  harus  mempunyai  mutu yang  tinggi, terutama  kemurnian  dan kehalusannya.Untuk itu, Indonesia masih  mendatangkan  kalsium  karbonat murni dari luar negeri dalam jumlah yang cukup besar. Bittern merupakan bahan buangan industri garam yang disebut juga air tua, mengandung senyawa kalsium. Karbon dioksida biasanya berasal dari hasil pembakaran yang masuk ke udara. Kandungannya di udara kecil, tetapi berpotensi sebagai pencemar. Dengan mereaksikan kalsium yang terkandung dalam bittern dengan gas CO2 akan terbentuk CaCO3 dalam suasana basa. Pembentukan kalsium karbonat dilakukan dengan proses kontinyu dalam reaktor kolom bersekat miring. Dari hasil penelitian diperoleh bahwa pengendapan magnesium dengan larutan ammonia menyebabkan kandungan kalsium ikut terdegradasi. Hasil terbaik yang diperoleh dicapai pada kondisi pH awal, kecepatan alir gas CO2, kecepatan alir cairan, dan suhu masing-masing pada 8,7; 2265 mL/menit; 10 mL/menit; dan 303 K, dengan konversi sebesar 38,40%. Produk berupa CaCO3, yang diperoleh mempunyai kemurnian sebesar 21,34%.

  6. AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI DUWET (Syzygium cumini Linn. PADA PEROKSIDASI LIPIDA SECARA IN VITRO

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    Rohadi Rohadi

    2016-04-01

    Full Text Available All parts of Syzygium cumini Linn. (duwet were widely used for medicinal plant in the treatment of various deseases. The seed extract was used to lower blood glucose. Cumini's seed had higher phenolic fractions than others. It were prepared by extracted of duwet seed "Genthong" varieties, using various extractants such as 85% ethyl acetate, 50% methanol and 50% ethanol. Duwet seed extract collected then was determined of phenolics compound and antioxidant activity assayed by measuring 2,2-diphenyl-1-picrylhydrazylradical (DPPH scavenging activity, reducing Ferric ion (Fe3+ power and inhibition of linoleic acid oxidation. The objective was to select one of the extractant which gave highest yield and polyphenolic content and its stronger antioxidant activity. Extractant 50% methanol gave highest the extract yields was 16.29 % (db. and phenolics compounds of extract was composed: total phenolic 45.99±0.25 g-GAE/100 g-extract; total flavonoid 2.28±0.07 g-QE/100 g-extract and total tannin 26.9±0.07 g-TAE/100 g-extract. All of extract exhibited strong on behalf of RSA-DPPH assay 87-95% (100  g-mL-l and reducing power, nevertheless in inhibition of linoleic acid oxidation was moderate 49-55% (400  g-mL-l. Keywords: Syzygium cumini Linn, seed, antioxidant, extraction   ABSTRAK Tanaman duwet (Syzygium cumini Linn. pada semua kompartemennya dimanfaatkan masyarakat untuk pengobatan suatu penyakit. Ekstrak bijinya dimanfaatkan untuk penurun gula darah. Bijinya merupakan bagian tanaman yang kaya senyawa polifenol. Fraksi kaya senyawa fenolik dipreparasi dengan mengekstraksi biji duwet varietas "Genthong", dengan tiga jenis ekstraktan; etil acetat 85%, metanol 50% dan etanol 50%. Ekstrak biji duwet (EBD yang diperoleh dianalisis kelompok senyawa fenolik dan aktivitas antioksidan menggunakan metode uji penangkapan radikal DPPH (2,2-diphenil 1-picrylhydrazyl, uji reduksi ion Feri (ferric reduction antioxidant power-FRAP dan uji penghambatan peroksidasi asam lemak linoleat. Tujuan penelitian adalah memilih satu dari tiga ekstraktan yang menghasilkaan ekstrak, dengan yield dan kadar senyawa polifenolik terbesar serta sifat antioksidatif terkuat. Yield dengan ekstraktan Met-OH-50%, sebesar 16,29% (db, senyawa fenoliknya sebesar 45,99 ±0,25 g-GAE/100 g-EBD; 2,28±0,07 g-QE/100 g-EBD dan 26,9±0,07 g-TAE/100 g-EBD. Ketiga ekstrak kuat dalam uji penangkapan radikal DPPH antara 87-95 % (100 g-mL-l dan uji reduksi ion Feri (Fe3+, moderat pada uji penghambatan peroksidasi lipid, 49-52 % pada 400  g- mL-l. Kata kunci: Biji duwet (Syzygium cumini Linn., antioksidan, ekstraksi

  7. Pengaruh Rasio Aditif Polietilen Glikol Terhadap Selulosa Asetat pada Pembuatan Membran Selulosa Asetat Secara Inversi Fasa

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    Cut Meurah Rosnelly

    2012-06-01

    Full Text Available Preparation of cellulose acetate (CA membranes with ultrafiltration process had be done by phase inversion using dimethylformamide (DMF as solvent. Poliethylene glycol (PEG 1450 Da, as additive, was added with 10, 20, and 30% rasio of celluose acetate. The thin film of polymer solution was immersed on water bath coagulation at room temperature. The analysis of membrane morphology structure by Scanning Electron Microscope (SEM JSM – 5310 LV, Jeol-Japan showed the asymetric of membrane. The addition of PEG can improve the performance of the membrane. In resulting flux is higher than membranes without PEG. Increasing of PEG/CA ratio resulted in the higher flux with lower of rejection. The higher fluxes of water, dextran, and BSA are 146, 114, and 96 L/m2hr with 52,938 and 75,716% rejection for dextran and BSA. Keywords: cellulose acetate membranes, polyethylene glycol, ultrafiltration

  8. ANALISIS KUALITATIF KANDUNGAN KIMIA KALUS Sonchus arvensis L. HASIL PERTUMBUHAN SECARA KULTUR JARINGAN

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    Katno Katno

    2012-10-01

    Full Text Available Plant tissue culture tecnique can be used to produce chemical compound or secondary metabolites from medicinal plant. The chemical compound of medicinal plants sometime produced locally within the callus. These compounds are generally identical with the one produced by medicinal plantgrowth in the field. Sonchus arvensis (tempuyung used as diuretic and anti-kidney stone. The flavonoid compound of Sonchus arvensis related to such activities are Apigenin 7-glucoside and Luteolin 7-glucoside. The both compounds of Sonchus arvensis are active as anti-kidney stone and diuretic by plant tissue  culture. The reseach was carried out in plants tisue culture laboratory at BPTO Tawangmangu from June to Jully 2002. The callus of Sonchus arvensis  aged of 50 days was analized with spot test and TLC methode. The result was compared to the chemical compounds from Sonchus arvensis which growth in the field. The result showed that chemical compound from  the callus and the plant growth in the field were identical.

  9. Structural Assurance, Kepercayaan pada Sistem E-commerce dan Niat Bertransaksi Secara Online

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    Gandis Ayuningtiyas

    2011-08-01

    Full Text Available Structural Assurance, Trust and Intention to Make Online Transaction. The objective of this research is to examine some determinant factors that influence user intention to make online transaction by using the Theory Planned Behavior (TPB. This research done in Brawijaya University Malang. Researcher have got 264 respondents which is student of Brawijaya University and never done online transaction. Multiple regression technique was used in this analysis. The variables are structural assurance and trust. The result of analysis indicates that simultaneously, structural assurance and trust significantly influence to the intention to make online trans-action. The implication of this research is relevant toward on-line business sector to consider factors which influence the intention online transaction.

  10. VALIDASI PEMERIKSAAN INFEKSI MENULAR SEKSUAL SECARA PENDEKATAN SINDROM PADA KELOMPOK WANITA BERPERILAKU RISIKO TINGGI

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    Endang R. Sedyaningsih Mamahit

    2012-09-01

    Full Text Available Accurate and adequate treatment of STIs is a critical component of STI-control activities to reduce transmission and sequelaes. On the other hand, chronic shortage in skilled staff and laboratory equipment in many countries necessitate the use of clinical skills more in order to diagnose and differentiate STIs. For these places, the WHO has recommended and produced a protocol of it syndromic approach management of STIs in place of treatment by -etiology. Since 1997 the Indonesia Ministry of Health has been conducting national training on this method. However, the syndromic approach for vaginal discharge is known to be problematic since differentiation among cervicitis, vaginitis, and even normal condition is difficult. The main objective of this study is to determine the sensitivity, specificity and positive predictive value of the syndromic approach management of women with signs and/or symptoms of abnormal vaginal discharge. The sample population were women with high risk sexual behaviors in East Java and North Sulawesi provinces. The laboratory tests using DNA hybridization probe technique (the PACE 2 test, Gen-Probe, San Diego, Calif. for Neisseria gonorrhoeae and Chlamydia trachomatis were used as gold standard. In addition, we also compared the clinical approach widely used by clinicians (mainly at hospitals with laboratory results. A total of 439 participants was recruited purposively (230 from E. Java and 209 from N.Sulawesi. In E.Java, the sensitivity, specificity, and predictive value of the syndromic management for vaginal discharge are 31%, 83%, and 59%, respectively, and in N.Sulawesi 49%, 56%, and 40%,respectively. The clinical approach did not show better results. In E.Java the sensitivity, specificity, and positive predictive value are 13%, 89%, and 50%, res­pectively, while in N.Sulawesi they are 42%, 61%, and 39%, respectively. As a conclusion, the current form of syndromic management has little use for STI screening among high risk women. Further studies by adding more criteria to the syndromes are needed to improve this method.

  11. Pemanfaatan Ragi Tapai dan Getah Buah Pepaya pada Ekstraksi Minyak Kelapa secara Fermentasi

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    Cut Erika

    2014-02-01

    ABSTRACT. In Aceh, coconut oil extraction is traditionally done through a natural fermentation of fresh coconut meat to produce simplah and pliuk u oil. Simplah oil and pliek u oil have high level of moisture content, free fatty acids, and peroxide value due to long fermentation process. The study was conducted by using the fresh shredded coconut meat. Then mixed with tapai yeast and papain from immature papaya fruit sap and were fermented for 48, 72, and 96  hours respectively. The yield of coconut oil produced in this study was from 8.53 % - 19.0 %, moisture content 12.58 - 2.66 %, free fatty acid content 3.29 % - 11:55 %, and peroxide value 0.76 - 1.75 mg O2/g of oil sample. The highest yield of coconut oil was obtained in the control treatment which significantly different from other treatments. The highest moisture content and free fatty acid were obtained on the treatment of using tapai yeast during fermentation process of coconut oil and did not meet the requirements set by the Indonesian National Standard (SNI 01-2902-1982, while the peroxide value of coconut oil produced in various treatment have met the criteria of SNI. The average organoleptic score of coconut oil color 3.38 (from moderate to clear which the highest score was obtained on fermented coconut oil with the addition of tapai yeast. The highest hedonic score of aroma was found in the coconut oil fermented with the addition of papain from papaya fruit sap with fermentation time of 48 hours is 2.9 (from dislike  to moderate.

  12. Urgensi Pemilu Presiden dan Wakil Presiden Secara Langsung di Era Reformasi

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    Abu Tamrin

    2016-04-01

    Full Text Available Abstract: the Importance of Direct Presidential and Vice Presidential Election in Reformation Era. Direct president and vice president election has begun in 2004 based on Act Number 23 Year 2003and renewed by Act Number 42 Year 2008. The importance of this provision is that elected president and vice president reflect the choice of majority of people. Beside of preventing problems in Parliament, this provision is also aimed to keep the sustainability of President both as head of government and head of State So,  it would not be easy for him to be removed or impeached. DOI: 10.15408/jch.v1i2.2990

  13. Pengaruh hide poison dan formalin terhadap sifat kulit dalam pengawet kulit kambing secara dikeringkan

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    Ignatius Sunaryo

    1991-06-01

    Full Text Available The objective of this study is know the effect of ide poison and formaline in the dried curing of goat skin. This study used 199 goat skins that were devided into seven groups od curing. Each group consisted of 17 goat skins. Those groups were : 3 groups of dried curing by hide poison of 0,1%, 0,3% and 0,5%; 3 groups of dried curing by formaline of 1%, 3% and 5%; and 1 groups of dried curing without any preserevation agent as control. After having stored for about 4 months, those skins then to be analized. Physical and chemical analysis werw carried out to evaluate the quality of the skin. Completely randomized design by Duncan’s test was used to analyze the dta. Based on this statistical analysis we can know that: the effect of hide poson in the dried curing of goat skins was the best. The quality of goat skins that were cured by hide poison was as follows : - the average tensile strength was the highest (886,30 kg/CM2, - the average of stretch was the lowest (14,50%, - the average iof hardness was the highest (201,90 kg/2,54 Cm, - the water content was the lowest (20%, and the damage was the smallest (10%.

  14. IMPLEMENTASI KEBIJAKAN LAYANAN SECARA ELEKTRONIK PENGADAAN KENDARAAN DINAS PEMERINTAH PROVINSI JAWA BARAT

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    Yusa Djuyandi

    2013-09-01

    Full Text Available Electronic Procurement Service (LPSE is a policy in the procurement of goods and services with internet-based, which facilitates a transparent auction. West Java was chosen as one of the 5 LPSE Center and expected to create transparency and accountability in government management. The purpose of this study is to describe and analyze the LPSE policy implementation, particularly in the procurement of official vehicles in West Java Provincial Government. The research was conducted through observation and interview techniques. Data consisted of primary and secondary data, therefore this research design used qualitative methods. The theory used in this study was the theory of policy implementation. Based on the research, it is known that the policy implementation of LPSE, particularly in the procurement of official vehicles in West Java Provincial Government, is not only able to minimize the corruption potential, but also able to provide a positive impact on the financial condition of local governments. This research conclusion is LPSE policy implementation running well.

  15. Penggunaan Kromium Organik dari Beberapa Jenis Fungi terhadap Aktivitas Fermentasi Rumen Secara in Vitro

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    W.D. Astuti

    2006-12-01

    Full Text Available Chromium appears to be an essential trace element since 1959, but its effect on ruminal microbes is not clear yet. This experiment was conducted to study the effects of organic chromium supplementation on rumen fermentation activity. An in vitro technique was held using randomized block design with 13 treatments and 3 replications. There were four kinds of organic Cr used, produced with four different species of fungi as carriers. Fungi used as carriers were Saccharomyces cerevisiae, Aspergillus oryzae, Rhizophus oryzae and “ragi tape”. The result indicated that the optimum organic Cr supplementation was 1 mg organic Cr/kg dry matter. Supplementation of 1 mg organic Cr/kg dry matter increased dry matter and organic matter digestibilities. It also tended to increase NH3 and total VFA production. Propionate production increased, which decreased methane production and increased hexose conversion efficiency in several treatments. Each fungus used as carrier of organic Cr resulted in different effects on rumen fermentation activity, but the effects was within a normal range. It was concluded that either Saccharomyces cerevisiae, Aspergillus oryzae, Rhizophus oryzae or “ragi tape” could be used as carrier in organic Cr production.

  16. Formulasi Niosom Ketoprofen Dan Prediksi Penggunaan Transdermal Secara In Vivo Pada Kelinci

    OpenAIRE

    Ismail, Isriani; Wahyudin, Elly; Rahman, Latifah

    2013-01-01

    Niosom adalah system vesikel yang dapat digunakan sebagai pembawa obat lipofilik, hidrofilik dan ampifilik. Ketoprofen adalah salah satu golongan AINS yang sangat sukar larut dalam air dan dalam pH cairan lambung, paparan lama pada mukosa lambung dapat memperparah ulcus, sehingga bahan obat patut dikembangkan dalam bentuk sediaan lain dengan rute yang lebih nyaman, misalnya dalma bentuk sedian transdermal. Penelitian ini bertujuan untuk memformulasi niosom yang dapat menjerap ketoprofen secar...

  17. Membran Polisulfon untuk Pengolahan Air: Dengan dan Tanpa Pra Perlakuan Koagulasi secara Ultrafiltrasi

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    Bastian Arifin

    2014-06-01

    Full Text Available Membran polisulfon untuk pengolahan air telah dilakukan untuk mengurangi warna air dengan proses koagulasi dan tanpa proses koagulasi. Membran ultrafiltrasi polisulfone telah dikarakte-risasi dengan mengukur fluks, permeability (Lp, dan MWCO dengan bebagai variasi berat molekul dekstran. Morfologi membran diobservasi dengan scanning electron microscopy (SEM. Analisis SEM dilakukan terhadap permukaan membran dan penampang melintang membran. Proses koagulasi optimum dilakukan dengan menggunakan alat jar test, diperoleh kondisi optimum pada 50 ppm Al2(SO43 dan pH 7. Indeks warna rejeksi diperoleh dengan koagulasi adalah 85% dan tanpa koagulasi adalah 62%.

  18. Hidrolisis Pati Gadung (Dioscorea hispida Dennts) Secara Enzimatis Pada Pembuatan Sirup Glukosa

    OpenAIRE

    Siregar, Meiyanni Adawiyah

    2015-01-01

    Some type of Dioscorea that grown in Indonesia have not been fully utilized, one tuber gadung. Gadung ( Dioscorea hispida Dennts) is a kind of tuber that grows wild int the woods and not difficult to get and potentially be used as glucose syrup. Hydrolysis of starch into glucose syrup can be done with acid or enzymes. In the hydrolysis process to make glucose syrup in composed of two phase, liquefaction and saccarification. Glucose syrup is sweetened foods and beverages in the form of a liqui...

  19. BUSSINESS PROCESS REENGINEERING SISTEM PRESENSI MAHASISWA BERBASIS WAKTU SECARA PAPERLESS (STUDY KASUS : STMIK POTENSI UTAMA

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    Fhery Agustin

    2014-10-01

    Full Text Available Bussiness Process Reengineering is a reconstruction concept with dramatically and radically action to generate improvements process, cost savings, and increasing speed. Student Presence System of STMIK Potensi Utama that running today, after the evaluation it has many weakness in terms of 5 indicators that cause the need for Bussiness Process Reengenering. This study aims to make the Reengineering process to produce an enhancement if it compared with the system before the “Bussiness Process Reengineering” did using 3 approach (restructure, informate, and mind. Based on the result of the research, there’s no increasing of the input category, 66.67% increasing of process category, and 50% increasing of the output category. So overall, the Bussiness Process Reengineering that has been done on students presence system of STMIK Potensi Utama can be said “it’s working good”. The new system that’s generated by the concept of Bussiness Process Reengineering in STMIK Potensi Utama is “The Prototype of the Web-Based Paperless Students Presence Applications” with the time limit method.

  20. Analisa Kuantitatif Dobi (Deteration Of Bleachability Index) Dalam Minyak Sawit Secara Spektrofotometri

    OpenAIRE

    Zuherawan, Chandra

    2011-01-01

    DOBI (Deteration Of Bleachability Index) representing index of degree of raw palm oil paleness and represent the parameter determining quality of Crude Palm Oil (CPO). High price DOBI from palm oil conducted by Spektrofotometri, so that can fulfill the standard of quality of departmental exporting of Commerce and Industry. From analysis result can be compared to by DOBI Crude Palm Olein in practice to DOBI Crude Palm Olein theoretically, where obtained by bigger DOBI Crude Palm Olein Theoreti...

  1. ANALISIS RASIO ENERGI DAUR ULANG PANAS PADA PRODUKSI BIODIESEL SECARA NON-KATALITIK

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    Armansyah Halomoan Tambunan

    2012-08-01

    Full Text Available Energy consumption in non-catalytic biodiesel production is still high, and needs to be reduced to the optimum level. It can be accomplished by recirculating the heat being used in the process by using heat exchanger. The objective of this study is to analyze the energy ratio of the system as influenced by the heat recirculation through a heat exchanger. This experiment used a superheated methanol vapor method for non-catalytic biodiesel production. The study was started with the determination and calculation of physical and thermal properties of materials to be used (palm olein, methanol, and methyl ester, continued with the designing of the heat exchanger, the experiment itself, and the energy ratio analysis. The process was occured in semi-batch mode with 3 levels of methanol flow rate, i.e., 1.5, 3.0, and 4.5 mL/minute, at reaction temperature of 290 °C. The results show that heat recirculation by using heat exchanger can increase the energy ratio from 0.84 to 1.03, according to the definition that energy ratio is the ratio between energy content of the biodiesel to the total energi of the feedstock and the process energy. If the energy ratio is defined as the ratio between the increase in energy content of the biodiesel from its feedstock to the process energy, the energy ratio was found to be 7.85, 2.98, and 2.87 for the respective methanol flow rate of 1.5, 3.0, and 4.5 mL/min.

  2. Efek Imunostimulator Propolis terhadap Proliferasi Limfosit T dan Viabilitas Sel Tumor Mammae Mencit secara in Vitro

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    ERMA MUSBITA TYASTUTI,

    2006-05-01

    Full Text Available The development of new medicines as immunostimulator agent has beingincreased according to the development of lethal diseases, for example tumor that suppress the immune responses. Propolis, which is resinous substance produced by honeybees known to have antimicrobial, anti- inflammatory, antioxidant, immunostimulator, and anti-tumor activities. The aims of this experiment were to study the effect of propolis as immunostimulator agent in proliferation of mice-suffered with mammary tumor T lymphocyte and the mammary-tumor cells’ viability after been treated with stimulated T lymphocyte. Three different dozes of propolis used in this experiment were 0.5 µg/mL, 1.5 µg/mL, 4.5 µg/mL, and added in cell culture to measure the proliferation of T lymphocyte. Proliferation activity of T lymphocyte was examined with 3-(4.5-dimethyl-2-thiazolyl-2.5-diphenyl-2H-tetrazolium bromide (MTT assay. Data was analyzed byusing Analysis of Variance (ANOVA then followed by Duncan Multiple Ranges Test (DMRT 5%. The result showed that the proliferation of T lymphocyte was increased after addition of propolis. The highest OD of T lymphocyte was reached in addition of propolis 4.5 µg/mL (2.363. In the examination of mice-mammary tumor cells’ viability, stimulated T lymphocyte was added to tumor cells culture and the results showed that the viability of tumor cells was decreased according to the increasing of T lymphocyte proliferation. The lowest tumor cells’ viability was reached in addition of stimulated T lymphocyte with propolis 4.5 µg/mL (4.667.

  3. TATA GUNA LAHAN DALAM TINJAUAN PENYUSUNAN KEBIJAKAN DAN PENGELOLAANNYA SECARA ISLAMI

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    Aldrin Yusuf Firmansyah

    2013-09-01

    Full Text Available Land use is still one of the key elements of urban design. It determines the basic two dimensional plans on which three dimensional spaces are created and functions are performed. Urban is also a fusion of architecture, economics, history, geography, politics and religion. This entire aspects make a city has complex form. Islam as an amalgamation on community life, it contributes urban order include policy and management of land use. This paper has a purpose to explore Islamic contribution on arrangement of land use policy and management, from philosophical level to operational level. This paper uses methodical interpretative-historical research. It means investigations into social-physical phenomena that are a past condition, within complex contexts, with a view toward explaining those phenomena in narrative form and in a holistic fashion.

  4. Pengurangan Kadar N dan P pada Limbah Cair Laboratorium secara Fitoremediasi Menggunakan Enceng Gondok (Eichornia crassipes

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    Nurmaya Arofah

    2016-03-01

    Full Text Available Laboratory liquid waste contains both metal, nonmetal, organic and anorganic content. If it is discharged directly to environment, it will cause pollution that is harmful for living thing. Data of waste survey result in 2009-2010 indicated that liquid waste of Laboratory in Chemical Engineering Department, Gadjah Mada University (JTK UGM consist of various complex compound such as NH4+, PO43-. JTK UGM has wastewater treatment plant (WWTP to deal with environmental pollution coming from waste of each laboratory and have three process, anaerobe activated sludge, aerobe and phytoremediation. Because the laboratory’s waste was very complex, the WWTP have not operated maximally yet. So, it is necessary research on wwtp processing step, particularly for phytoremediation process using water hyacinth. Objective of this research was to study effect of concentrations N and P on adaptability and capability of water hyacinth to grow. In addition it developed a mathematical model to estimate of N and P concentration decrease rate in liquid. The research was done in batch by planting water hyacinth that has been acclimated in wastewater containing N-NH4OH and P-H3PO4. Parameter observed is N and P concentration in liquid. The result indicated that water hyacinth can absorb N and P. Water hyacinth grow well in N-NH4OH concentration of 9.651 ppm and 15.573 ppm and P-H3PO4 concentration of 2.013 ppm and 2.7373 ppm. It was marked with additional weight and colour of water hyacinth leaves of fresh green. Mathematical model presented to estimate N concentration decrease rate indicated good result in experiment. However, P concentration decrease in liquid phase, the presented model is still not good, because P nutrient decomposition process has not been accommodated during absorption by water hyacinth.

  5. Analisa Kuantitatif Asam Laurat Dalam Produk Asam Lemak PT. Soci Secara Kromatografi Gas

    OpenAIRE

    Ginting, Zulkifly

    2009-01-01

    Asam lemak merupakan asam monokarboksilat dengan rantai yang tidak bercabang dan mempunyai jumlah atom karbon genap. Kadar asam lemak yang terdapat pada produk asam lamak FA1299(asam laurat 99% minimum) mengandung asam lemak jenuh yang berasal dari bahan baku minyak inti sawit dan minyak kelapa. Produk asam lemak FA1299 diperoleh dari proses hidrogenasi selanjutnya difraksinasi sehingga dihasilkan campuran asam lemak yang akan dianalisa dengan kromatografi gas. Hasil analisa produk mengandung...

  6. Pemodelan dan Prediksi Daya Ouput Photovoltaic secara Real Time Berbasis Mikrokontroler

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    Eka Prasetyono

    2015-09-01

    Full Text Available The electrical energy generated by the photovoltaic (PV as a renewable energy source highly affected by environmental conditions such as intensity of sunlight irradiance, temperature, geographic location and tilt angle of PV itself. How much power should be generated by the PV for every times and anywhere will be discussed in this paper. This paper are implemented models of clear sky solar irradiance, solar position and PV temperature to predict the power output should be generated by PV. The model is implemented on the ARM Cortex M4F microcontroller STM32F407 which is a 32bit microcontroller and equipped with DSP, so the prediction of PV power output can be done online and in real time. To be able to predict the PV power output online, at any time and wherever they are, in this study microcontroller equipped with temperature sensors and input geographical information (latitude-longitude and also equipped with a memory card for data logger between the predictions and field measurement. Results have been obtained by field experiments, measurements test for PV is very close to predictions with an average error 4.72% and computation time for all models by microcontroller with DSP instruction 33.64% faster compare to without DSP instruction.

  7. VALIDASI PEMERIKSAAN INFEKSI MENULAR SEKSUAL SECARA PENDEKATAN SINDROM PADA KELOMPOK WANITA BERPERILAKU RISIKO TINGGI

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    Endang R. Sedyaningsih Mamahit

    2012-09-01

    Full Text Available Accurate and adequate treatment of STIs is a critical component of STI-control activities to reduce transmission and sequelaes. On the other hand, chronic shortage in skilled staff and laboratory equipment in many countries necessitate the use of clinical skills more in order to diagnose and differentiate STIs. For these places, the WHO has recommended and produced a protocol of it syndromic approach management of STIs in place of treatment by -etiology. Since 1997 the Indonesia Ministry of Health has been conducting national training on this method. However, the syndromic approach for vaginal discharge is known to be problematic since differentiation among cervicitis, vaginitis, and even normal condition is difficult. The main objective of this study is to determine the sensitivity, specificity and positive predictive value of the syndromic approach management of women with signs and/or symptoms of abnormal vaginal discharge. The sample population were women with high risk sexual behaviors in East Java and North Sulawesi provinces. The laboratory tests using DNA hybridization probe technique (the PACE 2 test, Gen-Probe, San Diego, Calif. for Neisseria gonorrhoeae and Chlamydia trachomatis were used as gold standard. In addition, we also compared the clinical approach widely used by clinicians (mainly at hospitals with laboratory results. A total of 439 participants was recruited purposively (230 from E. Java and 209 from N.Sulawesi. In E.Java, the sensitivity, specificity, and predictive value of the syndromic management for vaginal discharge are 31%, 83%, and 59%, respectively, and in N.Sulawesi 49%, 56%, and 40%,respectively. The clinical approach did not show better results. In E.Java the sensitivity, specificity, and positive predictive value are 13%, 89%, and 50%, res­pectively, while in N.Sulawesi they are 42%, 61%, and 39%, respectively. As a conclusion, the current form of syndromic management has little use for STI screening among high risk women. Further studies by adding more criteria to the syndromes are needed to improve this method.

  8. Penelitian penggunaan monomer n-butil akrilat untuk peningkatan mutu kulit secara iradiasi

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    Dwi Wahini Nurhajati

    1996-06-01

    Full Text Available Research on the utilization of n-butyl acrylate monomer for quality improvement leather by irradiation cobalt-60 gamma-rays is carried out as follows: Javanese crust hide was impregnated with water emulsions of n-butyl acrylate monomer for 2 hours, packed in to poly ethylene bags, sealed, then irradiated by cobalt-60 gamma rays (doses : 5 to 25 kGy. The irradiated leather was washed by water, dried and then physical tested. The results of physical test of leather modified with n-butyl acrylate showed the increasing of tensile strength and elongation, decreasing of water absorption, resistance against the flexing test of 20,000 times, and PH was constant. Organoleptic test showed that the softly of the modified leather was no different with unmodified leather.

  9. ANALISIS EKSTRAK TUMBUHAN REMPAH SEBAGAI PRESERVATIVES MAKANAN TAHU DIUJI SECARA IN VITRO

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    ni luh putu manik widiyanti

    2017-01-01

    Abstract Tofu is know as a traditional food and as a source of high quality protein that contains many essential amino acids and is proven to reduce levels of Low Density Lipoprotein. Tofu susceptible to decomposition by bacteria, because high of the protein content and water content.  But many food entrepreneurs that naughty by adding formalin and methanyl yellow dye that banned its use in food regulation by Minister of Health Num. 1168/Menkes/PER/X/1999 for the food preservatives of tofu. The alternative is an antibacterial plants and used as a natural food preservatives including Cymbopogan ciratus (lemongrass, Cucurma domestica (turmeric and Alpinia galanga (galangal to inhibit bacterial growth in tofu.  Tofu that has been created is still not meet the assessment standards of SNI 01-3142-1992 in aroma part, whereas 50% panelists who stated aroma tofu is slightly acidic. Organoleptic test of tofu in first day of soaked with natural preservatives, 100% panelists who stated tofu that soaked with three kinds natural preservatives with aroma and taste of Cymbopogon ciratus (lemongrass, Cucurma domestica (turmeric and Alpinia galanga (galangal extracts. The characteristic color  of tofu, 100% panelists expressed that tofu with pure white color or pure yellowish and 100% panelists who stated normal of tofu in performance without slimy and moldy. In fact characteristic of tofu is decrease of quality result from organoleptic test on day three and six both in aroma, taste, color and performance of tofu. Gain score result show that tofu soaked with natural preservatives lemongrass concentration 20% that show increase bacteria in number. On third and six days, tofu soaked with 40% and 60% concentrations of lemongrass inhibit growth of bacteria. Result gain score indicates  tofu that soaked with tumeric extract on first and sixth days soaked with 20%, 40% and 60% concentrations   show all of tumeric extract concentrations didn’t inhibit growth of bacteria. On the third day tofu that soaked with only 60% concentration capable to inhibit growth of bacteria.   Result gain score  indicates tofu that soaked with extract of galangal in first day, only concentration 40% show inhibit growth of bacteria. On third and sixth days, all of concentrations of galangal extract didn’t capable inhibit growth of bacteria. Number of SPC bacteria in tofu soaked with preservatives lemongrass with concentration 40% was significantly different with tofu that soaked extract of 20% concentrations of turmeric and galangal.  Number of SPC bacteria in tofu soaked with lemongrass concentration 60% was significantly different with 20% concentrations of both turmeric  and galangal, and 40% concentration of galangal. Number SPC of bacteria in tofu soaked with 20% concentration of turmeric was significantly different with 40%  and 60% concentrations of  lemongrass. Number SPC of bacteria in tofu soaked with 20% concentration of galangal, was significantly different with 40% and 60% concentrations of lemongrass. Number SPC of bacteria in tofu soaked with 40% concentration of galangal was significantly different with 60% concentration of preservatives lemongrass with p<0,05. Inhibition zone by 20% and 40% concentration of lemongrass was significantly different compared with inhibition zone by both 60% concentrations of turmeric and galangal toward growth of bacteria. Inhibition zone by 60% concentration of lemongrass was  significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibition zona by 20% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 40% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 60% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibition zone by 20% and 40% concentrations of galangal was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 60% concentration of galangal was significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibiton zone was present in growth of bacteria caused by active substances in three kinds of natural preservatives. Coeffisient phenol test was done that show three kinds  of natural preservatives was extracted by hydrodestillation with value equal or less effective than pure phenol.   Key words : spices plants, natural preservatives, tofu, in vitro test.

  10. Analisis dan Perancangan Website Pembelajaran Bahasa Secara Mandiri di Self Access Language Learning Center Bina Nusantara

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    Hady Pranoto

    2010-12-01

    Full Text Available The purpose of this research is to build an independent language learning applications using Internet technologies to facilitate the user in learning the language. The research method used is by analyzing the ongoing learning process; analyze the constraints and limitations of existing facilities and designing new processes to address existing constraints and limitations. In designing a language self learning website authors examine the existing through observation, interviews and questionnaires to the stakeholders who use the system. Observations also observed duration of the user in conducting learning activities, and provided space to accommodate the transaction. After analysis of the current system, author designing the new system using object-oriented design methods and using analysis tools UML. After completed the system design done system built using ASP.NET programming language. The Conclusions is an independent language learning applications using Internet technology giving easy way to facilitate the learning process in terms of accesing time and place, and the application also provide facilities for teacher to monitoring student self learning. 

  11. PENGARUH PEMBERIAN POVIDONE IODINE DAN IODIUM TINCTURE SECARA TOPICAL TERHADAP FUNGSI KELENJAR TIROID

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    Suryati Kumorowulan

    2012-11-01

    Full Text Available ABSTRACT Background: Iodine Deficiency Disorder (IDD is one of the main nutritional problems in Indonesia. The classic manifestation of IDD is goiter endemic and cretinism.  Efforts have been conducted to eliminate IDD such as oral iodine supplementation and salt iodization.  An alternative method that had been tried was through topical medication using iodine containing solution such as iodine tincture or povidone iodine.  Both solutions were relatively cheap and almost available in every drug shop.  This study was to explore the medicine to eliminate IDD which is rubbing self treatment and the danger of excessive iodine uptake is minimized. Objectives: The study was to investigate the effect of povidone iodine and iodine tincture by topical usage to the thyroid gland function. Methods: The study design was quasi experiment trial with pre- post design.  Location of study was Srumbung and Dukun Subdistricts of Magelang District, Central Java.  The subject was women of child bearing age (15–45 years with visible goiter and normal thyroid stimulating hormone (TSH level or hypothyroid. The subject were divided into two groups, each group consisted of 30 women of child bearing age. Group I was rubbed with povidone iodine on the neck   every two days for 2 months and, group II was rubbed with iodine tincture with the same scheme as the first group. The level of TSH, free thyroxine (free T4 and urinary iodine excretion (UIE were compared before and after intervention. Results: TSH level on povidone group (Group I decrease from 3,6 μU/L (before to 2,17 μU/L (after intervention.  On tincture group also decrease from 2,7 μU/L to 1,42 μU/L. The median of free T4 on povidone group increase from 0,86 ng/dl  (before to 1,03 µg/dl (after intervention, whereas on tincture group the median of free T4 also increase with small value. The median of UIE on povidone group decrease from 383 μg/L (before to 130 μg/L (after intervention, whereas on tincture group increase from 235 μg/L to 311 μg/L. The size of neck circle on povidone group showed that no significant difference between before and after intervention, whereas the tincture group had significant difference.  Conclusions: The topical usage on thyroid gland of topical povidone iodine and iodine tincture improved TSH and free T4 levels. The treatment with iodine tincture decreased the size of goiter. [Penel Gizi Makan 2007, 30(1: 25-30]   Keywords:  povidone iodine, iodine tincture, TSH, free T4, UIE.

  12. PELAKSANAAN PENGADAAN BARANG/JASA SECARA ELEKTRONIK (E-PROCUREMENT PADA PEMERINTAH KOTA YOGYAKARTA

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    Dwi Haryati

    2011-06-01

    Full Text Available Yogyakarta municipality is committed to improve the quality of its procurement procedures by applying electronic procurement system, which finds its standing on Mayor Regulation Nr. 13 of /2009. Two issues are going to be addressed: first, whether the purpose of e-procurement has been served and second, what are the obstacles that occur in its implementation. Pemerintah kota Yogyakarta berkomitmen untuk meningkatkan kualitas pelaksanaan pengadaan barang dan jasa pemerintah dengan mengunakan sistem electronic procurement yang menggunakan Peraturan Walikota Yogyakarta No. 137 Tahun 2009 sebagai landasan hukum. Tulisan ini akan mengelaborasi pertanyaan apakah tujuan pelaksanaan e-procurement telah tercapai serta kendala apa sajakah yang menghambat pelaksanaan tersebut.

  13. PEMANFAATAN TEKNOLOGI INFORMASI DALAM RANGKA MEMBERANTAS TINDAK PIDANA KORUPSI SECARA ELEKTRONIK

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    Edi Nugroho

    2014-09-01

    Full Text Available radication of corruption in repressive/enforcement/penal would be more effective if accompanied by efforts in preventive/precautionary/non penal. One of the sources of corruption that resulted in losses to the state and the country’s economy is in terms of government procurement of goods and services. Efforts to prevent corruption in the procurement of government goods and services, as well as efforts to create good governance and free from corruption, collusion and nepotism in providing services to the public, is to utilize information and communication technology, in the form of e-government and e-procurement, as part of efforts to eradicate corruption in Indonesia.

  14. Pengukuran Kapasitas Antioksidan Dalam Rimpang Temulawak (Curcuma Xanthorrhiza Roxb.) Secara Spektrofotometri Sinar Tampak

    OpenAIRE

    Maggie

    2015-01-01

    One of Indonesia’s top natural product, Java turmeric (Curcuma xanthorrhiza Roxb), is very strategic to be developed, considering the benefits demonstrated by one of its active compound which is polyphenols. The presence of the phenolic group leads to strong antioxidant activity in biological systems, therefore could prevent diseases associated with reduction-oxidation reaction. The purpose of this study was to determine the antioxidant capacity in Java turmeric dosage form in the market and...

  15. HUBUNGAN SENSISTIVITAS PLASMODIUM FALCIPARUM TERHADAP KOMBINASI PIRIMETAMIN/SULFADOKSIN DAN KLOROKUIN SECARA IN VITRO

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    Sahat Ompusunggu

    2012-09-01

    Full Text Available An in vitro sensitivity test was conducted to study the sensitivity of Plasmodium falciparum against chloroquine and pyrimethamine/sulphadoxine combination. The relationship between sensitivity of the parasite to the two drugs was also studied. A total of 72 patients from five localities were examined during 1984-1985. Test against chloroquine was conduc­ted according to WHO method, while against pyrimethamine/sulphadoxine combination, a modified method of Nguyen Dinh and Payne and Eastham and Rieckmann was used. The results showed that there is no relationship between the sensitivity of P. falciparum against pyrimethamine/ sulphadoxine combination and chloroquine. It can be concluded that in case of chloroquine resistant P. falciparum, pyrimethamine/sulphadoxine combination could be applied as an alternative chemotherapy.

  16. AKTIFITAS ANTIHELICOBACTER DARI LACTOBACILLUS SP SECARA INVITRO : POTENSI SEBAGAI SUATU BAHAN BIOTERAPI TUKAK LAMBUNG

    OpenAIRE

    Zaraswati Dwyana

    2007-01-01

    Salah satu upaya yang dilakukan adalah mencari bahan bioterapi alami yang dapat digunakan dalam pengobatan terhadap infeksi Helicobacter pylori. Tujuan penelitian ini ingin mengetahui kemampuan Lactobacillus sebagai bahan bioterapi dengan mengetahui aktivitas antimikrobanya terhadap Helicobacter pylori yang kemudian dapat disebut antihelicobacter. Lactobacillus ditumbuhkan media tumbuh dan difermentasi lalu disentrifus sehingga diperoleh kultur lactobacillus cair lalu diliofilikasi untuk...

  17. Penetapan Kadar Tramadol Secara Titrasi Semi Bebas Air Dalam Sediaan Kapsul

    OpenAIRE

    2016-01-01

    Many drugs circulating in the market to reduce or eliminate the pain are called analgesic, one of the drug used were tramadol capsules. Tramadol HCl is a bronsted acid having pKa value of 8.3 was titrated with strong base using solvent mixture of water and ethanol as much as tramadol base soluble in water and ethanol function disolves tramadol base. In the manufacture of pharmaceutical dosage from the drug must accordance with requirements of the fourth edition of the pharmacopoeia Indonesia ...

  18. PENYUSUNAN FORMULAS! PENGGUNAAN PUPUK RAMAH LINGKUNGAN PADA PADI SAWAH UNTUK PERLINDUNGAN LAHAN PERTANIAN SECARA BERKELANJUTAN

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    I MADE ADNYANA

    2015-06-01

    Full Text Available The research was done in the area of subak Tibuhbiyu, Kerambitan District, Tabanan Regency to get the rational suitable fertilizer formulation as a environmentally friendly on rice plant. To achieve these objectives, the research conducted through several activities, namely: soil survey, physical and chemistry of soil analysis, the factorial field experiment of rice plant response for fertilizer treatments, and analysis of the soil quality after harvest. Most of Subak Tibuhbiyu paddy soil was a medium soil fertility status (60%, due to the high value of the cation exchange capacity (CEC and base saturation (KB. Provision of compost can also reduce the use of NPK fertilizers. The best formulation fertilizer are 10 tons ha-1 compost in combination with 75% of the NPK recommendation, which can provide a high grain yield, ie 7.35 tones ha-1. In general, added of NPK fertilizer, organic fertilizer, or the each other combination, not cause pollution to the soil environment, because its had a medium quality of soil after harvest.

  19. PENENTUAN KANDUNGAN LOGAM Cd DAN Cu DALAM PRODUK IKAN KEMASAN KALENG SECARA SPEKTROFOTOMETRI SERAPAN ATOM (SSA

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    Hellna Tehubijuluw

    2013-05-01

    Full Text Available Determination of cadmium and copper concentration of canned fish have been done. Some canned fished were taken away from three mercks circulating in a market and marked as RS (the 1st sample, NF(2nd sample, and CP(3rd sample. The aim of this research is to determine of  cadmium (Cd and copper (Cu content of canned fish and to compare the content with the standard limit regulated by the Directorate General of Drug and Food Control No: 03725/B/SK/VII/89. Determination of metal concentration was conducted using  atomic absorption spectrophotometer (AAS. It was found that Cd concentrations of RS and  NF samples are 0.1969 mg/kg and0.0448 mg/kg, respecively while for  CP it was not detected. For Cu concentrations of  RS, NF, and CP are 3.3303 mg/kg, 4.6130 mg/kg, 3.3047 mg/kg  respectively. The results indicated that the samples of the canned fishes  have been contaminated with Cd and Cu metals although the levels of those metals are still lower than  the maximum limit regulated by the Directorate General of Drug and Food Control No: 03725/B/SK/VII/89 where the maximum limit for Cd and  Cu are 0,2 mg.kg and 5.0 mg/kg respectively.    

  20. Model EPQ Multi Item yang Dimodifikasi untuk Dua Permintaan secara Simultan

    Directory of Open Access Journals (Sweden)

    Taufiq Rahman

    2017-05-01

    Full Text Available Inventory is one of many factors of the business operation that need to be controlled by industries in order to improve efficiency, enhance productivity, and decrease the holding cost. The holding cost of inventories in supply chain contribute to 20% - 40% of the product value. It can be controlled by applying appropriate inventory model, such as EPQ/Economic Production Quantity and EOQ/Economic Order Quantity. EPQ is an inventory model that used to determine the optimum production lot size with balanced the production setup cost and holding cost. Even the classic EPQ has applied widely in industries, the assumption used by this model differed between the researchers whether it is continuous or discrete demand, because the multi delivery or discrete demand is mostly used by industries. Even so, there are industries that used both continuous and discrete demand simultaneously. Based on previous research, there was an advanced EPQ model that synchronizing both assumptions simultaneously, but it still addressed single item problem. Since almost the industries produced multi item, this model has lack of applicability. Therefore, this research proposed a multi item EPQ Model that synchronizing continuous and discrete demand simultaneously. The solution procedure that used in this proposed model are classical calculus method/differential calculus and simultaneous approach. A numerical example is given to show the effectiveness of the proposed approach based on the data from the literature.

  1. PERBANDINGAN KADAR FLAVONOID EKSTRAK BUAH MAHKOTA DEWA (Phaleria macrocarpa [Scheff] Boerl SECARA REMASERASI DAN PERKOLASI

    Directory of Open Access Journals (Sweden)

    Indri Astuti Handayani

    2016-03-01

    Full Text Available One of which is an antioxidant. In the previous studies stated the fruit mahkota dewa containing several active compound, one of which is flavonoid. There are two kind of extraction method of decision compound efficacious contained in the crude drugs by using solvent is hot ways and cold ways. Remaceration and percolation method of withdrawal that can attract flavonoid compound found in fruit botanicals mahkota dewa. Research done by extracting fruit simplisia of mahkota dewa then compared the level of flavonoid contained there in with two different extraction methods. The results showed that the yield of maceration extract an average of 17.3264 % and 15.3846 % of percolation . Statistical analysis showed no significant difference in the yield value of Remaceration and percolation extract with p value = 0.676 . Results of the assay of flavonoids extract of 2,184 mg remaceration and percolation of 2,060 mg . The test results showed no statistically significant differences in the levels of flavonoids extract maceration and percolation with p value = 0.878 .

  2. Analisis Dimensi Fraktal untuk Identifikasi Tanaman dengan Pendekatan Pemrosesan Citra secara Paralel

    Directory of Open Access Journals (Sweden)

    Mohamad Solahudin

    2010-10-01

    Full Text Available The use of camera vision and real-time computation as plant identification tool has become an active research. Application of both methods can not be separated from a pretension that the computation must produce correct results within the specified time interval where the truth of the calculation depends not only on logical truth but also on the time in which results are produced. The purpose of this study is to identify plants with fractal dimension analysis and the application of parallel computing. Fractal dimension analysis results showed that each plant has a typical Fractal dimension value. Filtration process with a small window size is accompanied by the use of multiple processors indicates that the image processing in parallel show results much faster than processing with a single processor.

  3. PENGOLAHAN LIMBAH BUANGAN INDUSTRI TAHU MENGGUNAKAN BIOREAKTOR BIAKAN MELEKAT SECARA ANAEROB-AEROB

    OpenAIRE

    Yudha, Dwi Ancella

    2013-01-01

    Most tofu industries in Makassar is home industries that do not pay attention to the waste before disposal, especially liquid waste which most potentially contaminate the surrounding environment, especially water bodies because they contain high organic matter. Therefore, we need some process that can degrade organic materials contained in it, one of which is the use of biological treatment reactors or called with attached growth bioreactor (Attached Growth Bioreactor) in which the role of mi...

  4. Aktivitas Penghambatan Trichoderma spp. Formulasi terhadap Jamur Patogen Tular Tanah secara In vitro

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    S. M. Widyastuti

    2002-07-01

    Full Text Available The research was aimed to evaluate the growth of formulated conidia propagules of Trichoderma koningii, T. reesei and T. harzianum, which had been stored for 1 and 5 months and the inhibition activity against soil borne plant pathogenic fungi Rigidopous lignosus, Ganoderma sp., Fusarium sp., and Sclerotium rolfsii. The isolates were formulated in the forms of alginate bead, alginate-peat bead, and peat-lime powder. The results indicated that Trichoderma formulated stored at room temperature showed higher inhibition activity than that stored at 4oC. After 5 months in the storage, T. reesei in both bead and powder formula performed higher growth inhibition activity against soil borne pathogens than the other isolates tested. It was noted that all isolates performed temporary decreased growth inhibition activity against soil borne pathogens. Key words: Trichoderma formulation, soil borne pathogens

  5. Penggunaan Accelerometer MMA7361 sebagai Alternatif Pengukuran Lendutan pada Jembatan Secara Nirkabel Berbasis ATmega32

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    Mohd Syaryadhi

    2016-03-01

    by ATmega32. The system was tested by manually loading the trial bridge with 1 m of length. The loading deflects down the bridge to maximum 15 cm from the reference point. Sensor readout data was sent wirelessly using ZigBee real time to computer in a graphical display for easy analysis. The research give an alternative method in vertical deflection measuring on the bridge that can be utilized by stakeholder in policy decision.

  6. Parcels and Land Ownership, COGO and parcel map digitizing, primary attribute is APN, Published in 2000, Eureka County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Parcels and Land Ownership dataset, was produced all or in part from Other information as of 2000. It is described as 'COGO and parcel map digitizing, primary...

  7. A Triple-Error-Correcting Cyclic Code from the Gold and Kasami-Welch APN Power Functions

    CERN Document Server

    Zeng, Xiangyong; Hu, Lei

    2010-01-01

    Based on a sufficient condition proposed by Hollmann and Xiang for constructing triple-error-correcting codes, the minimum distance of a binary cyclic code $\\mathcal{C}_{1,3,13}$ with three zeros $\\alpha$, $\\alpha^3$, and $\\alpha^{13}$ of length $2^m-1$ and the weight divisibility of its dual code are studied, where $m\\geq 5$ is odd and $\\alpha$ is a primitive element of the finite field $\\mathbb{F}_{2^m}$. The code $\\mathcal{C}_{1,3,13}$ is proven to have the same weight distribution as the binary triple-error-correcting primitive BCH code $\\mathcal{C}_{1,3,5}$ of the same length.

  8. EVALUASI POSISI MAKSILA DAN MANDIBULA SECARA SEFALOMETRIK SETELAH PERAWATAN ORTODONTI CEKAT BERDASARKAN ANALISIS STEINER DAN McNAMARA (LAPORAN PENELITIAN

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    D. S. Hartono

    2015-07-01

    Full Text Available The purpose of this study was to evaluate the changes of maxillary and mandibular sagital position relative to cranial base. The subject of this research was patient with bimaxillary protrusion having orthodontic treatment and four first premolars extraction. The maxillary and mandibular position changes were measured by angular and linear parameters. Angular parameter as described by Steiner's analysis were SNA and SNB angles. Linear parameter as described by McNamara's analysis were point A and Pogonion relative to nasion perpendicular. The results of both groups was compared statistically and differences quantified using paired t-test. There was no significant changes in position of maxillar relative to cranial base. On the other hand, significant changes were found in position of mandibula relative to cranial base.

  9. PEMANFAATAN LIMBAH BATANG PISANG KEPOK (Musa paradisiaca formatypica) SEBAGAI BAHAN DASAR PEMBUATAN BIOETANOL DENGAN METODE HIDROLISIS SECARA ASAM DAN FERMENTASI

    OpenAIRE

    ., Sulfiani; Abd.Karim; Natsir, Hasnah

    2017-01-01

    Abstrak. Bioenergi, diantaranya bioetanol, adalah salah satu bentuk energi alternatif yang dapat memenuhi kebutuhan energi masa depan. Bioetanol adalah etanol yang dihasilkan melalui proses fermentasi terhadap glukosa dari bahan alam yang mengandung komponen pati dan selulosa. Penelitian ini bertujuan untuk memanfaatkan batang pisang kepok, yang memiliki kandungan selulosa sebesar 64%, guna menghasilkan bioetanol dengan cara hidrolisis asam dan fermentasi bakteri menggunakan bakteri Clostridu...

  10. PEMBACAAN POSISI KOORDINAT DENGAN GPS SEBAGAI PENGENDALI PALANG PINTU REL KERETA API SECARA OTOMATIS UNTUK PENAMBAHAN APLIKASI MODUL PRAKTIK MIKROKONTROLER

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    Herlambang Sigit Pramono

    2015-02-01

    Full Text Available ABSTRACT The purpose of this research is to add GPS application on practicum module of microcontroller for autoimatic railway barrier application. The method of this research is experimental design by designing the equipment of railway barrier based on GPS coordinates to develop modul application of microcontroller practicum. Data were collected from observation and measurement; the function from each part of the tool was observed while the latitude and longitude coordinates of the point in some locations were measured by the made tool. The data were analyzed by descriptive analysis for the function of each part of the tool and the measurement of coordinates were analyzed its erorr. The findings of the research showed that GPS could give the information about the position for both latitude and longitude coordinates if the accepted satelite signal met the standard requirement. Then, compared with the tools in market, the result from the made tool had error level of 0.82’ for latitude coordinate and 101,4’  for longitude coordinates. Keywords: GPS, microcontroller

  11. Uji Efek Antibakteri Ekstrak Etanol Kulit Batang Salam {Syzigium polyanthum (Wight Walp} terhadap Staphylococcus aureus dan Escherichia coli secara Invitro

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    Ikhsan Amanda Putra

    2015-05-01

    Full Text Available AbstrakTumbuhan salam (Syzygium polyanthum (Wight Walp. telah dikenal sejak dahulu untuk mengobati berbagai penyakit. Bagian tumbuhan yang dapat dimanfaatkan sebagai obat selain daun adalah bagian kulit batang. Beberapa penelitian yang telah dilakukan terbukti bahwa daun salam memiliki efek antibakteri. Tujuan penelitian ini adalah untuk mengetahui bagaimana efek antibakteri dari kulit batang salam. Penelitian ini bersifat deskriptif menggunakan metode difusi agar. Konsentrasi ekstrak yang digunakan adalah 25%, 50%, 75%, dan 100%. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit batang salam memiliki efek antibakteri terhadap Staphylococcus aureus dengan konsentrasi 25%, 50%, 75%, dan 100% memberikan daya hambat terhadap pertumbuhan bakteri sebesar 12 mm, 13,67 mm, 12,33 mm, dan 9 mm, sedangkan pada konsentrasi yang sama untuk Escherichia coli tidak terlihat daya hambat terhadap pertumbuhan bakteri. Konsentrasi ekstrak yang paling efektif dalam menghambat S. aureus adalah konsentrasi 50%, dimana konsentrasi 75% dan 100% kurang efektif.Kata kunci: uji efek antibakteri, kulit batang salam, staphylococcus aureus, eschericia coli AbstractSalam plants (Syzygium polyanthum (Wight Walp. Salam plants have been known since ancient to treat various diseases. The parts of the plant that can be used as drug are bark. From the research that has been conducted has proven that Salam leaves has an antibacterial effect. The objective of this study was to determine the antibacterial effect of salam bark.This was a descriptive study by using agar diffusion method. The concentration of the extract used was 25%, 50%, 75%, and 100%. The results showed that the ethanol extract of Salam bark has antibacterial effects to Staphylococcus aureus with the concentration of 25%, 50%, 75%, and 100% gives the inhibition of the growth of bacteria on 12 mm, 13.67 mm, 12.33 mm, and the 9 mm, while at the same concentration for Escherichia coli was no bacterial inhibition area. The most effective concentration of extract in inhibiting S. aureus was concentration of 50%, while the concentration of 75% and 100% less effective.Keywords: antibacterial activity test, salam bark, staphylococcus aureus, eschericia coli

  12. Analisis Perbandingan Kadar Kalium Pada Daun Kumis Kucing, Daun Pegagan Dan Daun Salam Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Sinaga, Lusi Ayulita

    2015-01-01

    The Orthosiphon aristatus (Blume) Miq, Centella asiatica (L) Urb and Syzygium Polyanthum (Wight) Walp Leaves empirically has been adopted by local community for treatment of hypertension in Tanah Jawa district Simalungun. These three herbals are recognized healing to hypertension patients mainly to stabilize mineral potassium in body. The plant has own group, since Orthosiphon aristatus (Blume) Miq into lamiaceae, Centella asiatica (L) Urb into Apiaceae and Syzygium Polyanthum (Wight) Walp in...

  13. PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

    Directory of Open Access Journals (Sweden)

    Dyah Widiastuti

    2013-09-01

    Full Text Available ABSTRACTDengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infectedAedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main targetof surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an earlywarning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash usingimmunocytochemical streptavidin biotin peroxidase complex (SBPC assay is an alternative method for dengue vectorsurveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in headsquash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. Theimmunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash todetect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assaycan detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive resultsfound in immunocytochemical SBPC assay.Key Word: Dengue, immunocytochemistry, DSSE10

  14. Penentuan Aktivitas Biologis Tanin Beberapa Hijauan secara in Vitro Menggunakan ’Hohenheim Gas Test’ dengan Polietilen Glikol Sebagai Determinan

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    A. Sofyan

    2008-04-01

    Full Text Available Leaves from trees are alternative source of forage for ruminant’s feed. However, most of the leaves contain high concentration of phenolic compounds, especially in the form of tannins. This experiment was aimed at quantifying biological activity of tannins using in vitro gas production method without and with the addition of polyethylene glycol (PEG. The leaves used in this experiment was Salix alba, Rhus typhina and Peltiphyllum peltatum. Several rumen fermentation variables, such as organic matter digestibility (OMD, metabolizable energy (ME and total VFA production were measured. The results showed that crude protein, NDF and hemicellulose contents of Salix alba leaves were the highest, while there was no difference in ADF content from the others. Biological activity of tannins in Salix alba, Rhus typhina and Peltiphyllum peltatum were 0.7%, 45.7% and 122.6%, respectively. There was a significant correlation between total phenols and tannins’ biological activity (r=0.70; P<0.05, whereas no significant correlation was found for total tannins and condensed tannins. It was concluded that the addition of PEG increased in vitro gas production, organic matter digestibility, metabolizable energy and total VFA production after 24 hours incubation period.

  15. Sistem Keamanan Berlapis pada Ruangan Menggunakan RFID (Radio Frequency Identification dan Keypad untuk Membuka Pinta Secara Otomatis

    Directory of Open Access Journals (Sweden)

    Muhammad Chamdun

    2014-10-01

    Full Text Available This final project aims to design a door security device using RFID technology (Radio Frequency Identification and the keypad as a method of access into the room. The system will automatically give warning when the code is entered incorrectly or burglars entered by breaking the door. The microcontroller that is used as the central data processing is ATMega 16 microcontroller ATMEL production and security sensors are used PIR (Passive Infra Red and magnetic switches. The design begins with a schematic device hardware and software design using CodeVisionAVR programming. Implementation phase includes the implementation of the physical tools, programs, and sensors. The testing phase is done by testing the input sensors and exit switches, siren output devices, DC motor, LCD (Liquid Crystal Display, the RFID reader, keypad, and system controller. The results of room layered security system works according to design. PIR sensors can detect human activities and turn the LED lamp indicator on. Magnetic switch sensors will provide input to the microcontroller to enable or disable the DC motor as the room locking system. Admin menu that is accessed from the keypad is to add users, remove users, see registered users and change the password. User menu which is also accessed using the keypad, work as the room access using a username and password. This study resulted in the layered security. Room access is done using RFIDTag and password. The room piercing detection is done by sensor PIR (Passive Infra Red and magnetic switches. Alarm used as a piercing action warning. This layered room safety device produces a better security system.

  16. Penetapan Kadar Vitamin C Dari Bawang Putih (Allium sativum L.) Secara Titrasi 2,6-Diklorofenol Indofenol

    OpenAIRE

    2011-01-01

    Garlic is one of the best agricultural commodity considered to have a very good marketing prospect, it is related with the high consumption rate of garlic by Indonesian people as food seasoning. Morever, garlic also contains many non sulphurous compound, alixin, that has antioxidant, antimicrobial, antitumor properties. Garlic is a rich source of calcium, phosphorus and selenium. Also, garlic is an excellent source of vitamin C, vitamin B6 and mangan. Garlic sample were from one of tradit...

  17. Isolasi dan Identifikasi Secara Molekuler Ganoderma spp. yang Berasosiasi dengan Penyakit Busuk Pangkal Batang di Kelapa Sawit

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    Maria Indah Purnamasari

    2013-04-01

    Full Text Available A number of Ganoderma spp. isolates was isolated from oil palms attacked by basal stem rot (BSR disease in Padang and Pontianak plantations. Genetic polymorphism of these isolates was analyzed based on the internal transcribed spacer (ITS sequence of ribosomal DNA region. In addition, a restriction fragment length polymorphism (RFLP analysis was also performed to determine the association of the isolates with BSR disease. The isolated Ganoderma spp. showed high DNA polymorphism and there was no obvious genetic clustering of isolates that may correspond to their geographical position in Padang and Pontianak. This indicated that exchange of DNA between Ganoderma spp. infecting oil palm is not uncommon. This can be explained by the heterothallic nature of Ganoderma spp. in which DNA recombination occurs during sexual reproduction between different thalli. RFLP analysis showed that ITS fragments from all Ganoderma spp. isolates were digested with restriction enzymes MluI and SacI. This indicated that the anoderma spp. isolates were specific for oil palm and thus associated with the BSR.Key words: basal stem rot, Ganoderma spp., internal transcribed spacer, oil palm

  18. Daya Antibakteri Air Jeruk Nipis (Citrus aurantifolia) Terhadap Pertumbuhan Stapylococcus aureus dan Escherichia coli yang Diuji Secara In Vitro

    OpenAIRE

    Nurkalimah, Cut

    2012-01-01

    Antibacterial power is the ability to inhibit growth or kill bacteria. to determine the antibacterial power of a substance can be carried out sensitivity tests of bacteria to these substances in vitro. This research aims to determine the antibacterial power of lime (Citrus aurantifolia) on the growth of Stapylococcus aureus and Escherichia coli tested in vitro. The method used is a laboratory experiment. Sensitivity test performed on two groups of bacteria that is Stapylococcus aureus a...

  19. PENGARUH PERKEMBANGAN TEKNOLOGI INFORMASI TERHADAP KEABSAHAN PERJANJIAN DALAM PERDAGANGAN SECARA ELEKTRONIK (E-COMMERCE DI ERA GLOBALISASI

    Directory of Open Access Journals (Sweden)

    Lathifah Hanim

    2011-02-01

    Full Text Available Economic activity in the entire world with the existence of existence is increasingly dependent telematic technology. The development of Information technology giving influence on the validity of contracts in electronic commerce (E-Commerce in the era of globalization and the what’s factors constraints on the validity of contracts in electronic commerce (E-Commerce in the era of globalization. Article 1320 Civil Code is the principal instrument to test the validity of contracts made by the parties. Article 1320 Civil Code there are four legal conditions of contract, namely: agreements, the ability to create engagement, a certain thing, and a lawful reason. Contracts that do not meet the agreement and skill, resulting in the contract to cancel and void by law, if there are contracts that do not qualify or do not have a specific object or cause is not allowed, the resulting contract is null and void.

  20. Perbandingan Reaksi Zat Besi Terhadap Teh Hitam dan Teh Hijau Secara In Vitro dengan Menggunakan Spektrofotometer Uv-Vis

    Directory of Open Access Journals (Sweden)

    Husnil Wardiyah

    2014-01-01

    Full Text Available AbstrakSalah satu permasalahan gizi yang dihadapi Indonesia adalah anemia defisiensi besi. Defesiensi besi ini dapat disebabkan oleh asupan dan serapan yang tidak adekuat, seperti kebiasaan mengonsumsi zat yang dapat menghambat penyerapan zat besi seperti minum teh pada saat makan. Hambatan penyerapan ini disebabkan oleh polifenol yang terkandung di dalam teh, terutama tanin. Penelitian ini dilakukan pada teh hitam dan teh hijau yang banyak dikonsumsi masyarakat. Larutan teh hitam dan teh hijau dijadikan sebagai kontrol, kemudian diberikan perlakuan dengan meneteskan FeCl3 1% sebanyak lima tetes. Larutan tersebut dibaca besar absorbannya dengan spektrofotometer UV-Vis. Prosedur ini dilakukan dengan pengulangan sebanyak lima kali. Data hasil penelitian diolah dengan menggunakan independent sample t test untuk melihat perbedaan rata-rata pada dua kelompok sampel tersebut. Dari hasil penelitian didapatkan rata-rata besar absorban teh hitam kontrol 0,539 dan setelah diteteskan zat besi 0,30640. Absorban teh hijau kontrol 0,961 dan setelah diteteskan zat besi 0,65020. Hal ini berarti bahwa terjadi penurunan konsentrasi larutan tersebut. Penurunan absorban pada kontrol teh hitam dengan perlakuan adalah 43,15%, sedangkan pada teh hijau adalah 32,34%. Berdasarkan uji statistik, disimpulkan bahwa terdapat perbedaan bermakna antara absorbansi teh hitam dan teh hijau.Kata kunci: Teh hitam, teh hijau, zat besi, absorban.AbstractOne of the nutritional problems faced by Indonesia is iron deficiency anemia. It is caused by inadequate intake and absorption. One of the causes of this inadequate absorption is eating habit to consume substances that can inhibit iron absorption like drinking tea while eating. This is caused by tea polyphenol compounds, especially tannins. Black tea and green tea were observed in this research since these are widely consumed by public. Solution of black tea and green tea were used as control and they were treated by giving five drops of 1% FeCl3. Absorbance of those solutions was read by using spectrophotometer UV-Vis. This procedure was done with five times repetition. The result of this research was processed by using independent sample t test to discern mean of two groups samples. The result showed that the absorbance mean of black tea control is 0.539 and the absorbance mean of black tea that has dripped by iron is 0.30640. As for the green tea control absorbance is 0.961 and absorbance mean after iron sheds is 0.65020. It means that there is a dilution and reduction of solution concentration. Based on these figures, it was concluded that the concentration of black tea with iron sheds is smaller than the concentration of green tea with iron sheds. Nevertheless, there is a greater reduction in black tea compare with green tea in the absorbance reduction. Absorbance reduction from black tea control group to treatment group is 43.15%, while absorbance reduction from green tea control group to treatment group is 32.34%. Based on statistical tests, it was concluded that there is a significant difference between the absorbance of black tea and green tea.Keywords: Black tea, green tea, iron, absorbance.

  1. Visualisasi Hasil Mesin Uji Tarik Gotech GT-7010-D2E dalam Bentuk Grafik secara Real Time

    Directory of Open Access Journals (Sweden)

    Anggoro Suryo Pramudyo

    2014-03-01

    Full Text Available Gotech GT-7010-D2E is a tensile testing machine used in Metallurgical Engineering Laboratory UNTIRTA. The machine are used both for practical and Materials Engineering specimen testing demanded by several industries. The machine can perform tensile tests well, but for the analysis is done manually, so it requires a lot of time and accuracy of the analysis results are not very accurate. Therefore, it’s needed a software that can analyze the results of the tensile test in the form of graphs in real time. Software visualization is created using Visual Basic 6.0 and data from tensile testing machine is stored using Microsoft Access database. The software is designed to record any characters that appear in the LCD on the control panel of a tensile testing machine and then process them to be a graphic. Based on the results of the implementation of the specimen plate and wire observations prove that the difference between the use of visualization software is more accurate and effective in their use of time, compared to manual observation that requires further analysis of the test results. In software visualization results of tensile test, the mechanical properties can be analyzed directly from the test results and graphs of data obtained in real time.

  2. Sistem kontrol inventori pemasokan barang Secara Real Time Menggunakan Vendor-Managed Inventory (VMI dan Short Message Service (SMS

    Directory of Open Access Journals (Sweden)

    Rinaldo Turang

    2016-01-01

    Full Text Available This research underlines the urgency of real-time stock replenishment control system for distributors using vendor-managed inventory. It is valuable in improving corporate performance in lowering inventory cost as well as making better customer service due to the cut made in ordering cycle. The system produces real-time information on stock available at the retailing stores. Stock replenishment can then be done by the distributor at the right time. Using Short Message Service (SMS as a one-way information channel from retailers to their distributor, the data of every transaction of the supervised item are processed by a rule-based forward-chaining inference system to determine the item’s availability in stores. Folowing steps in Rapid Application Development (RAD, the construction of this system takes place through business modeling, data modeling, process modeling of VMI system, then the application is generated and tested as a prototype. The results are: the stock replenishment control system are able to make transactional data at the stores transparent to the distributor, facilitating the continuation in stock delivery. System architecture reduces the time needed to access information about sales on supervised item by using SMS shortcut. This control system developed in stores can be operationally independent from the retailing systems. Rule-based forward-chaining inference technique processes facts on stock variations, resulting in stock replenishment notification such as normal, reserve, and depleted warning. Keywords: VMI, inventory; Stock replenishment; Rule-based reasoning; Forward-chaining system, RAD

  3. Penetapan Kadar Kalsium, Kalium, dan Natrium dalam Buah Nanas (Ananas comosus (L.) Merr.) Cayenne Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Sitanggang, Siska Sepdiana

    2013-01-01

    Pineapple is one of the tropical fruits those are popular in public to consume fresh fruit, is also main material of canned fruit industry and processed products such as jam, syrup, and others. It contains many important elements that needed by the body, they are water, protein, carbohydrate, fiber, and minerals such as calcium, iron, magnesium, phosphorus, potassium and sodium. The content of vitamin are vitaminC, thiamin, riboflavin, niacin and vitamin A. Food content pota...

  4. Strategi Kebijakan Pemanfaatan Jasa Lingkungan Air secara Berkelanjutan di Taman Nasional Gunung Ciremai Kuningan-Jawa Barat

    Directory of Open Access Journals (Sweden)

    Rismunandar Rismunandar

    2016-12-01

    Full Text Available Mount Ciremai National Park (TNGC requires institutional policies for managing environmental services. This study aims to determine the sustainable management policy strategy for water environmental services, based on ecological, social and economical assessment. The approach used in this study is "multidimensional scaling" (MDS through the analysis of RAP-JASLING AIR (Rapid Appraisal Sustainability Index for Jasling Air to determine sustainable and performance of the management; and analysis of prospective to determine the policy strategy. The results shows that the ecological dimension index for TNGC in three areas, namely southern and central regions is quite sustainable and less sustainable for northern region. The economic dimension index for three regions seems to be less sustainable. The social dimension index for the three regions is quite sustainable. Intervention and improvements in attributes of each dimension needs to be implemented in order to reach sustainability in the future. On the other hand, prospective analysis resulted in four key factors that influence the policy of water environmental services in TNGC area and possibly to become alternative strategy for management policies, namely: management support for TNGC, income of community living in surrounding TNGC, efforts to improvecommunity's income, and protection of water springs.

  5. Pembuatan Dan Evaluasi Secara In Vitro Emulsi Virgin Coconut Oil (Vco) Menggunakan Emulgator Tween 80 Dan Gom Arab

    OpenAIRE

    Silaban, Rutlin Valentina

    2015-01-01

    Background: Currently virgin coconut oil (VCO) is consumed orally as a functional food or a supplement but the VCO has a bad taste so that less acceptable by consumers. Therefore, it is necessary to formulate VCO into an emulsion that is more acceptable by consumers. Objective: The objective of this study was to prepared VCO and VCO emulsion. Methods: Preparation of VCO was conducted by fermentation using microbial yeast tempe (Rhizopus oryzae). The VCO produced was tested for water co...

  6. Analisis Kandungan Kalsium, Kalium, dan Magnesium pada Daun Kelor (Moringa oleifera Lam.) Segar dan Direbus Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Wahyuni, Sri

    2015-01-01

    Moringa oleifera Lam that we know as Moringa is the best-known species of the thirteen species of the genus Moringacae. Minerals contained in Moringa are calcium, copper, iron, manganese, magnesium, phosphorus, potassium, sodium, sulphur, and zinc. Moringa leaves as a source of vitamins and minerals can be consumed in a way cooked, or eaten raw or dried into powder. One of the cooking method is boiling in the water. Vitamins and minerals are easily soluble in water is a nutrient that is quick...

  7. Penentuan Kadar Triklosan Dalam Cairan Antiseptik Resik-V Manjakani Dan Pasta Gigi Formula Secara Kromatogarfi Cair Kinerja Tinggi

    OpenAIRE

    Yona S., Ayu Shilvya

    2015-01-01

    Determination levels of triclosan in Resik-V Manjakani antiseptics liquid and Formula toothpaste has been done. Determination levels of triclosan use triclosan liquid as an internal standar by using high performance liquid chromatograph. Determination was based on the peak area and retention time of triclosan standard. The result of this determination showed that Resik-V Manjakani antiseptic liquid contain 0,0955% triclosan and Formula toothpaste contain 0,10785% triclosan, because of that l...

  8. Ketersediaan Energi Ransum yang Mengandung Wheat Pollard Hasil Olahan Enzim Cairan Rumen yang Diproses Secara Steam Pelleting pada Ayam Broiler

    Directory of Open Access Journals (Sweden)

    W. W. Wardani

    2004-12-01

    Full Text Available Wheat pollard is a common feed ingredient, but its non starch polysaccharide component is still a limiting factor as an anti nutrition. The combination treatment of enzymes from rumen liquor (DE and steam pelleting (SP, could change non-starch polysaccharide structure to be more digestible for broiler chicken. Twenty seven broiler chicken of 37 days old were divided into 27 experimental unit and randomly offered one of 9 treatments, that were R1 (DE 0 U/kg + 60oC, R2 (DE 0 U/kg + 80oC, R3 (DE 0 U/kg + 100oC, R4 (DE 620 U/kg + 60oC, R5 (DE 620 U/kg + 80oC, R6 (DE 620 U/kg + 100oC, R7 (DE 1.240 U/kg + 60oC, R8 (DE 1.240 U/kg + 80oC and R9 (DE 1.240 U/kg + 100oC. The chicken have been fasted for 24 hours and fed by force feeding 30 g/head while water was offered ad libitum. Apparent Metabolizable Energy (AME, Nitrogen Corrected Apparent Metabolizable Energy (AMEn, True Metabolizable Energy (TME, Nitrogen Corrected True Metabolizable Energy (TMEn and Nitrogen Retention were determined. Three chickens were used for collecting nitrogen and endogenous energy. Data were analyzed by analysis of variance according to completely randomized design with factorial 3x3 and Duncan’s Multiple Range Test. Enzymes dosage and steam treatment did not influence metabolizable energy (AME, AMEn, TME and TMEn and nitrogen retention. Combination of enzymes (R4 and R8 and steam temperature treatment increased AME, AMEn, TME and TMEn but did not increase nitrogen retention. The result indicated that combination of enzymes at dosage of 620 U/kg with steam 60oC treatment increased the metabolizable energy of wheat pollard based diets.

  9. Pengawetan Kayu Gubal Jati Secara Rendaman Dingin dengan Pengawet Boron untuk Mencegah Serangan Rayap Kayu Kering (Cryptotermes cynocephalus Light.

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    Afif Sumaryanto

    2015-10-01

    The utilization of younger teakwood has a disadvantage, which is the sapwood is more susceptible to dry wood termites as it has less natural durability. Boric acid and borax are inexpensive preservatives, which contain boron as the active material. Those preservatives are also easy to be obtained as well as do not produce smells and the wood discoloration. The experiment materials were the sapwood parts from teak boards obtained from the felled trees in the community forest of Kali Bawang, Kulon Progo. A complete randomized block design was arranged in a factorial with two factors, which were the type of preservatives (boric acid and borax in 5% concentration and duration of cold soaking (12, 24, 36, and 48 hours. To examine the durability of sample, dry wood termites (Cryptotermes cynocephalus Light were used. The results showed that the average values of absorption, retention, and depth of penetration were 33.09 to 70.77 kg/m3, 3.81 to 10.77 kg/m3, and 2.34 to 3.86 mm, respectively. The average values of termite mortality during 2 weeks and 4 weeks were 46.33 to 53 %, 82.67 to 94.33 %. Weight reduction and degree of the damage were, 560 to 570 mg, and 30.34 to 31.27 %, respectively. By analysis of variance, there was an interaction between the type of preservatives and the duration of cold soaking factors, which affected significantly the termite mortality. Type of preservative affected significantly the absorption and penetration. Further, the duration of cold soaking affected significantly the level of absorption, retention and penetration. The application of preservatives could reduce the mass loss of specimens until 70 % as well as to give higher levels of mortality rate (87-92 % compared to that of untreated one.

  10. Penetapan Kadar Kalsium Dan Besi Pada Bubur Bayi Instan Yang Beredar Di Kota Medan Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Marpaung, Elsa Elfrida

    2015-01-01

    Kalsium dan besi merupakan mineral yang diperlukan oleh bayi yang sedang bertumbuh dan jumlah yang diperlukan melebihi jumlah yang diperoleh dari ASI. Oleh karena itu, bayi harus mendapat makanan tambahan disamping ASI. Makanan pendamping ASI seperti bubur susu merupakan sumber yang baik bagi beberapa vitamin dan mineral seperti kalsium dan besi dalam kadar tertentu yang cukup untuk memenuhi kebutuhan bayi dalam hal pembentukan tulang dan gigi serta hemoglobin. Tujuan penelitian ini adalah un...

  11. Karakterisasi Simplisia Daun Dan Biji Kelor (Moringa Oleifera Lam.) Serta Analisis Magnesium Dan Besi Secara Spektrofotometri Serapan Atom

    OpenAIRE

    2016-01-01

    Moringa leaves was used as vegetable by most of the villagers and also used as a medicinal plant efficacious. Nowaday people are starting to utilize parts of the moringa plant in the form of fresh are then processed into simplicia of moringa leaves, tea of moringa and simplicia of moringa seeds. Monograph of characterization simplicia of moringa leaves and seeds have not been listed in the Indonesian Herbal Pharmacapoeia. Minerals are nutrients needed to maintain health. This study aims to in...

  12. PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

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    Dyah Widiastuti

    2013-09-01

    Full Text Available ABSTRACTDengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infectedAedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main targetof surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an earlywarning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash usingimmunocytochemical streptavidin biotin peroxidase complex (SBPC assay is an alternative method for dengue vectorsurveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in headsquash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. Theimmunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash todetect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assaycan detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive resultsfound in immunocytochemical SBPC assay.Key Word: Dengue, immunocytochemistry, DSSE10

  13. Analisis Kandungan Mangan dan Zink Pada Daun Kelor (Moringa oleifera Lam.) Kering dan Rebus Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Faza, Afina

    2017-01-01

    Moringa (Moringa oleifera Lam.) is included in the family Moringaceae, the utilizaation of moringa plant in indonesia society has been a long a time, but moringa’s leaves is the most common used to be dietary fiber. Moringa contains excess nutrients for body needed, one of them is mineral such as calcium, cromium, cupri, flour, iron, manganese, magnesium, molybdenum, phosphor, potasium, sodium, selenium, sulphur, and zinc. The aim of this study is to determine the differentiation content of m...

  14. Sistem Keamanan Berlapis pada Ruangan Menggunakan RFID (Radio Frequency Identification) dan Keypad untuk Membuka Pinta Secara Otomatis

    OpenAIRE

    Muhammad Chamdun; Adian Fatchur Rochim; Eko Didik Widianto

    2014-01-01

    This final project aims to design a door security device using RFID technology (Radio Frequency Identification) and the keypad as a method of access into the room. The system will automatically give warning when the code is entered incorrectly or burglars entered by breaking the door. The microcontroller that is used as the central data processing is ATMega 16 microcontroller ATMEL production and security sensors are used PIR (Passive Infra Red) and magnetic switches. The design begins wi...

  15. REGENERASIRUMPUT LAUT Kappaphycus alvarezii (Doty MELALUI INDUKSI KALUS DAN EMBRIO DENGAN PENAMBAHAN HORMON PERANGSANG TUMBUH SECARA IN VITRO

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    Emma Suryati

    2009-04-01

    Full Text Available Regenerasi rumput laut Kappaphycus alvarezii dilakukan dalam rangka penyediaan benih yang bermutu dan mempunyai keunggulan melalui induksi kalus dan embrio dengan penambahan hormon pertumbuhan yang diintroduksi ke dalam media kultur yang dapat memacu induksi kalus dan penebalan pigmen rumput laut. Media kultur yang digunakan adalah media Conwy padat dengan penambahan agar 0,8%-1,6%. Hormon perangsang tumbuh yang digunakan untuk memacu pertumbuhan kalus dan filamen embrio yaitu IAA (Indol acetic acid, kinetin, dan auxilin dengan konsentrasi berkisar 0,4-1 mg/L. Embrio yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya. Sintasan dan perkembangan embrio yang paling baik yaitu dengan penambahan IAA dengan konsentrasi 0,4 mg/L pada media padat. Pembentukan anakan dilakukan dengan mengiris embrio dan menumbuhkan pada media cair yang diperkaya dengan hormon yang sama. Pemeliharaan anakan pada media kultur dilakukan hingga mencapai ukuran 2-3 cm. Regeneration of seaweed Kappaphycus alvarezii was done to provide high quality seed through callus and embryo induction using plant growth regulator which was introducted to the culture medium. This growth regulator can stimulate the callus induction procces and thickening the seaweed pigment. Applied medium culture was agar medium with 0.8%-1.6% concentration enriched with Conwy and the applied growth regulators were IAA (Indol acetic acid, kinetin dan auxilin with 0.4-1 mg/L concentration range. Resulted embryo has the same characteristics with the stock. The best survival rate and embryo growth was IAA treatment with 0.4 mg/L concentration. Formation of embryo was done by transferring them from solid medium to the liquid one with the same growth regulator treatment. The nursery of the seed in culture medium was carried out until it has reached 2-3 cm in size.

  16. Penetapan Kadar Besi dan Kalsium dalam Jamur Merang (Volvariella volvacea) dan Jamur Tiram (Pleurotus ostreatus) secara Spektrofotometri Serapan Atom

    OpenAIRE

    Ruhama, Putri Tri

    2014-01-01

    Mushroom is a vegetable food source of considerable potensial as a source of nutrition around us. Even some type of mushroom from nature has long been cultivated for human consumption. The mushroom has considerable potensial as a source of nutrious food for nutrients and minerals are high enough complete. Iron and calcium are found in mushroom and oyster mushroom can be used as an additional intake of prevention of anemia (lack of blood) and osteoporosis (bone loss). The purpose is to find ou...

  17. Studi Kandungan Mineral Kalium, Natrium, Magnesium Pada Selada (Lactuca sativa L.) Hidroponik Dan Non-Hidroponik Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Novin, Sondang

    2013-01-01

    Lettuce is one of the many cultivated plants in hydroponics. The method of hydroponics is cultivate method plants without the use of soil as a growing medium, but using water. The lettuce has a high mineral content to the needs of human beings is like minerals potassium, sodium, magnesium, calcium, phosphorus, iron, vitamin A, vitamin B, and vitamin C. In the extracellular fluid potassium and sodium is an essential cations play a role in pH balance and osmolarity. While magnesium plays an imp...

  18. Efektivitas Isolat Bakteri Yang Berasal Dari Berbagai Rhizosfer dan Bahan Organik Terhadap Patogen Penyakit Layu Secara In vitro

    OpenAIRE

    Kuswinanti, Tutik; Baharuddin; Sukmawati

    2013-01-01

    As the new regional development of potato, Tana Toraja District has the different climate, and types of soil compared with other regions. Rhizosphere is an ideal area for growth and development of microbial antagonists, caused of rhizosphere function as providers of nutrition. Some nutrients that secreted in the rhizosphere are influenced by many environmental factors. Based on these facts, the research were aimed to explore the diversity of bacteria from the rhizosphere of plants and organ...

  19. PERENCANAAN VIRTUALISASI LAYANAN PENGADAAN SECARA ELEKTRONIK PEMERINTAH KABUPATEN BADUNG MENGGUNAKAN METODE BLUE OCEAN STRATEGY DAN BALANCED SCORECARD

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    Made Aryawan

    2016-11-01

    Full Text Available Abstract --- The main obstacles holding LPSE in Indonesia today is the limited available resources (infrastructure and competent human resources so it needs proper governance to maintain the availability of service in order to increase the procurement credibility of government goods or services. Virtualization Technology Utilization proposed as an alternative solution considering the efficiency, effectiveness and ease of maintenance, is considered able to accelerate the realization of a reliable and accountable LPSE so it can increase the participation of service users. This research discussed the Planning of Virtualization based on Blue Ocean Strategy analyses (BOS and Balanced Scorecard (BSC, where the virtualization creates innovations value for LPSE Badung and becomes a real step towards BOS considering it has not been widely adopted by other LPSE. BOS gives a picture of the current organization and desired ideal condition, while the optimization and management of variable accuracy achieved through the implementation of Key Performance Indicators / KPI from various perspectives within the framework of the BSC. Canvas mapping strategy in this research, produce four steps framework namely: (1 the elimination step is to negate Internet access failure, (2 the step to reduce application error, access difficulties, the difficulties in operating the applications and connection interference, (3 the step to increase the availability of information, the speed and the smoothness of the transaction process, ease of complains, the quality of support services, data security system and (4the step to create  service interference resistance. The virtualization implementation also gives a positive value towards the performance of LPSE services in terms of user satisfaction level, where in general the achievement recorded close to the value of user expectation, even in the services related to the handling of complaints, the performance achievement showed the value that exceeds user expectation. Further optimization and virtualization planning accuracy, conducted through the establishment of 11 Key Performance Indicators / KPI in 6 perspectives is the development of the four main perspectives BSC framework.  

  20. Penambahan Molases Untuk Meningkatkan Kualitas Amoniasi Jerami Padi dan Pengaruhnya terhadap Produk Fermentasi Rumen Secara In-Vitro

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    Muhamad Bata

    2010-10-01

    Full Text Available Supplementation of molasses to improve the quality of rice straw ammonization and its effect on fermentation product in-vitro ABSTRACT. Aimed of this research was to find out the optimal level of molasses addition at straw rice ammonization process to N-NH3 production, VFA and microbe protein synthesis. Material used was rumen fluid of fistula cattle, rice straw, water, urea and molasses. Treatment tried was level of molasses addition 0%, 15% and 30% on rice straw which given urea. Research was carried out by experimental method as in vitro, was conducted use completely randomized design. Variable measured were N-NH3, VFA and microbe protein synthesis. Intake data entered in data tabulation and analyzed variance then continued by orthogonal polynomial test. Research result after ammonization indicated that acidity level and concentration of released NH3 decrease parallel with addition of molasses level, and also increase the nutrient content which was crude protein increase and crude fiber decrease. Variance analysis and Test of orthogonal polynomial result indicated that treatment of molasses addition have highly significant effect (P<0.01 and linier respond to concentration of released NH3 after ammonization. Research result as in vitro indicated concentration N-NH3 and VFA total decrease while microbe protein synthesis increase. Variance analysis result indicated that molasses addition treatment at straw rice ammonization process have highly significant (P < 0.01 on concentration of N-NH3, VFA total and Microbe Protein Synthesis. Test of orthogonal polynomial for molasses addition at straw rice ammonization process indicated linier respond on concentration of NH3 N-NH3 and VFA total, but microbe protein synthesis quadratic had respond (P < 0,01 white regression equation Y= 52.187-1.089222X + 0.11X2 (r2 87.27 and (r 0.9341. Research result could conclude that molasses addition up to level 30% able to improve quality of straw rice ammonization process by NH3 fixation so that increase nutrient ingredient, decrease NH3 that lost to atmosphere, improve utilization of N-NH3 and VFA and also increase microbe protein synthesis.

  1. PERTUMBUHAN KALUS DAUN DEWA [Gynura procumbens (Lour Merr.] DENGAN KOMBINASI 2,4-DICHLOROPHENOXYACETIC ACID DAN KINETIN SECARA INVITRO

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    Samkhatin Khaniyah

    2012-09-01

    Full Text Available Tanaman daun dewa [Gynura procumbens (Lour Merr.] berguna untuk menurunkan kadar kolesterol darah, mengobati diabetes, tumor, dan sebagai obat anti kanker. Daun dewa mengandung senyawa metabolit sekunder yaitu flavonoid, saponin, dan minyak atsiri. Oleh karena itu, perlu adanya kultur in vitro yang diharapkan mampu meningkatkan jumlah metabolit sekunder, maka perlu dilakukan penelitian tentang induksi kalusdengan penambahan kombinasi 2,4-Dichlorophenoxyacetic acid dan kinetin yang tepat dapat digunakan untuk menginduksi kalus daun dewa. Tujuan penelitian ini untuk mengkaji pengaruh konsentrasi 2,4-D dan Kinetin serta interaksi keduanya terhadap induksi kalus daun dewa. Analisis menggunakan anava dua arah dan uji lanjut Duncan. Parameter yang diamati adalah parameter persentase eksplan yang hidup, persentase berkalus, berat basah, kering kalus, serta tekstur dan warna kalus . Dari Uji Jarak Berganda Duncan diperoleh hasil tertinggi pada konsentrasi 2,4-Dichlorophenoxyacetic acid 0.5 ppm dan konsentrasi kinetin 1 ppm sebesar 33.33% pada parameter persentase berkalus.Daun dewa [Gynura procumbens (Lour. Merr.] plant can be used to lower the blood cholesterol levels, to treat diabetes and tumors, and may be used as an anti-cancer drug. Daun dewa plant produces of secondary metabolites such as flavonoids, saponins, and essential oils. An in vitro culture is a necessity to increase the quantity of secondary metabolites using callus induction with the addition of a combination of 2,4-Dichlorophenoxyacetic acid and kinetin to induce daun dewa callus. The research will ecamine the influence of 2,4-Dichlorophenoxyacetic acid and Kinetin in various concentrations and their interaction on callus induction of daun dewa. Data were analyzed using advanced two-way anova and Duncan test. The test showed that the highest yeield was obtained from the combination of 0.5 ppm 2,4-D and 1 ppm kinetin, where 33.33% of plants had callus.

  2. Pengendalian Nematoda Parasitik Tanaman secara Hayati dengan Bakteri Pasteuria penetrans: Inventarisasi, Pembiakan Massal, dan Uji Patogenisitas Isolat Bakteri

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    Mulyadi Mulyadi

    1995-12-01

    Full Text Available The research on “Biological control of plant parasitic nematodes by nematophagous bacteria (Pasteuria penetrans” was planned to be conducted within 3 years period, started in 1994/1995. In the first year research was done with the following objectives: 1 inventory on the isolates of P. penetrans, and 2 study on the mass production and pathogenicity test of P. penetrans. Survey of P. penetrans was done in the provinces of D.I. Yogyakarta, Central Java, East Java, and West Java. Soil and root samples were collected in this survey, plant parasitic nematodes were isolated using centrifugation and funnel and spray method. Mass production of P. penetrans was done with their host especially root-knot nematodes (Meloidogyne spp.. The bacterial infected nematodes were inoculated on tomato  planted on sterilized soil. Roots contain root-knot nematodes inhabiting spores of P. penetrans were used as inoculum source. The pathogenicity tests of the isolates were done using bioassay method and in the green house in Completely Randomized Design. The research results is as follows: 1 seventeesn isolates of P. penetrans were found in the survey, these isolates were pathogenic to root-knot nematodes, 2 mass production of P. penetrans was done with their host especially root-knot nematodes and 4 treatment with P. penetrans significantly reduced the root-gall caused by root-knot nematodes. Key words: biological control, parasitic nematodes, Pasteuria penetrans

  3. Pemodelan matematis pengurangan COD dalam air limbah industri penyamakan kulit secara adsorpsi kontinyu menggunakan abu terbang bagas

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    Agus Prasetya

    2013-06-01

    Full Text Available The objective of this research was to obtain suitable mathematical model for Chemical Oxygen Demand (COD removal originated from tannery wastewater using bagasse fly ash in continuous system. In the column experiment, effect of flowrate, concentration of wastewater, and bulk density were studied. Three models: Adams-Bohart, Thomas, and Yan were applied to experimental data to predict the breakthrough curve. The best model was evaluated using correlation coefficients. Yan model was found to give the most accurate to describe dynamic behavior of the column experiment. The best result was obtained at flowrate of 100 mL/min, concentration of 400 mg/L, and bulk density of 61 g/L. The Yan kinetic constant (k and the Y adsorption capacity (q were 0.3210 mL/mg/min and 17.0947 mg/g respectively and the Y correlation coefficient obtained was 0.9379.

  4. VIABILITAS BAKTERI PROBIOTIK IN-VITRO DAN PENGARUH PEMBERIAN AIR OKSIGEN TERHADAP PERTUMBUHAN BAKTERI PROBIOTIK SECARA IN-VIVO

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    Enok sobariah

    2012-03-01

    Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 The aim of this study were  to identify the in-vitro tolerance of pro-biotic bacteria to acid and bile salt condition; and  to prove a hypothesis that the supplementation of oxygenated water has a positive effect on the body weight of rat and on viability of pro-biotic bacteria.  The first study was carried out at PAU Laboratory of Bogor Agricultural University, while the second study was conducted at Department of Community Nutrition of Bogor Agricultural University and Microbiology Laboratory of Indonesia Institute of Technology. Forty five rats aged 6 weeks were divided into three groups, i.e., control group without probiotic (a0, Lactobacillus casei Shirota (a1, and Lactobacillus IS-7257 (a2.  Each group (consisting of 5 rats each has three different treatments, namely, control without oxygenated water (b0, 50 ppm oxygenated water (b2, and 80 ppm oxygenated water (b2. Oxygenated water was administered to the rats twice a day in the morning (3.25 ml and afternoon (3.00 ml. Observation was carried out on the body  weight of the rats, fecal lactic acid bacteria, coliform, and anaerob bacteria by plate counting, for 4 periods, i.e, prior to the treatment (C0, after three-day treatment (C1, after seven-day treatment (C2, and on the 10th day treatment or three days after washed out period. The results indicated that probiotic bacteria are resistant to acid and bile acid condition. Oxygen concentration in water has a significant positive influence on the body weight of rats towards viability of probiotic bacteria (p-level   The supplementation of  oxygenated water 50 ppm significantly increase the population of viable fecal lactic acid bacteria in L. casei Shirota and Lactobacillus IS-7257 groups after 3 and 7 days of treatment.  Lactobacillus IS-7257 gave better response than L. casei Shirota. The supplementation of oxygenated water 80 ppm significantly reduces the fecal coliform in-vivo in both L. casei Shirota and Lactobacillus IS-7257 groups (p-level Keywords: oxygenated water, probiotic bacteria, viability

  5. PENGARUH KONSENTRASI EKSTRAK ETANOL BUAH PARE (Momordica charantia L TERHADAP PERTUMBUHAN BAKTERI Shigella dysenteriae SECARA IN VITRO

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    Sri Rahayu

    2016-10-01

    Full Text Available One of the gastrointestinal diseases are dysentery, which is characterized by loose, watery stools with mucus and blood of more than 3 times per day. Dysentery caused by Shigella dysenteriae bacteria, the disease can be treated with an antibacterial nutritious plants, one of which is bitter melon. Plant part used is fruit. Bitter melon fruit (Momordica charantia L contains flavonoids which efficacious as antibacterials. This study aims to determine the effect of extract concentrations against the bacteria Shigella dysenteriae. Antibacterial activity was tested using the disc diffusion method with extract concentrations of 10%, 25% and 50%. Clear zone formed around the paper discs showed inhibition zone. Results showed the ethanol extract of bitter melon extract at concentration 10%, 25% and 50% showed their antibacterial activity against bacteria Shigella dysenteriae with average diameter of inhibition zone 8.3 mm, 9.3 mm and 11.7 mm. Increased concentration of bitter melon fruit extract could increased inhibition zone.

  6. Faktor yang Berhubungan dengan Tingkat Pengetahuan Orang Tua Tentang Pendidikan Seks Secara Dini pada Anak Sekolah Dasar (SD

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    Maryuni Maryuni

    2017-01-01

    Full Text Available Indonesian Child Protection Commission states that at least 45 children who are victims of sexual violence every month. Early sex education for children is essential to prevent sexual violence but many parents have opinion that sex education for children are taboo. This study aims to determine what factors are associated with the level of knowledge of parents against early sex education for children. This research was conducted in Primary School Kartika VIII-5, South Jakarta 2014. This research is descriptive analytic with cross sectional approach. The population in this study are parents of students in grade 1 in Primary School Kartika VIII-5 South Jakarta. Samples were selected by total sampling method resulting on 60 respondents. Collecting data using questionnaires. Data analysis techniques using chie square. Results showed that there were a significant relationship between education, social and cultural values, and exposure information with knowledge about sex education at an early stage, while the work are not related. In conclusions, this study factors was correlated with parents knowledge about early sex education for children in Primary School Kartika VIII-5 were education, social and cultural values, and exposure information. Expected for parents to improved information about early sex education for children, so parents have good knowledge abou t sex education.

  7. Seleksi Bakteri Berpotensi Probiotik dari Ikan Mas (Cyprinus Carpio Indegenous Jantho Berdasarkan Aktivitas Antibakteri secara In Vitro

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    Cut Yulvizar

    2014-06-01

    Full Text Available One of the alternatives to antimicrobials in disease control is probiotic bacteria as microbial control agents. This research was carried out to obtain indegenous isolates of probiotic bacteria from digestive tract Cyprinus carpio. Selection criteria were appointed based on the capacity to produce the antibacterial compound against the pathogen and grow at pH 2. The research was conducted from May to July 2014 at Microbiology Laboratory, Biology Department, UNSYIAH. The research was carried out by experiment laboratories. The planting of the bacteria was carried out with the pour-plate method in nutrient agar medium. The result show that there are six bacterial isolates varied morphological colony and cell and able to produce antibacterial compounds for active against A.hydrophila and E. tarda.

  8. Penetapan Kadar Vitamin C Dari Bawang Putih (Allium sativum L.) Secara Titrasi 2,6-Diklorofenol Indofenol

    OpenAIRE

    Putra, Azhar Aliza

    2011-01-01

    Garlic is one of the best agricultural commodity considered to have a very good marketing prospect, it is related with the high consumption rate of garlic by Indonesian people as food seasoning. Morever, garlic also contains many non sulphurous compound, alixin, that has antioxidant, antimicrobial, antitumor properties. Garlic is a rich source of calcium, phosphorus and selenium. Also, garlic is an excellent source of vitamin C, vitamin B6 and mangan. Garlic sample were from one of tradit...

  9. Penetapan Kadar Mineral Kalsium, Besi, Zink, Tembaga dan Mangan pada Buah Pepino (Solanum muricatum L.) Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Nurhadi

    2016-01-01

    Minerals have important role in maintaining of body functions and are found in fruits and vegetables. Pepino and eggplant are of the same family. Pepino originated from Andes Mountains in Peru and Chile, South America. In Indonesia, pepino was initially imported during the colonial period. Its shape is oval with various weights, depending on the size of the fruit. It contains useful nutrients for health, especially minerals and vitamins. The minerals contained in pepino are iron, zinc, copper...

  10. Penetapan Kadar Mineral Kalium, Magnesium, Kalsium dan Besi pada Buah Ceplukan (Physalis angulata L.) Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Muammar

    2016-01-01

    Physalis angulata L. (family-Solanaceae) or it has been known as cutleaf groundcherry (ceplukan), an annual shrub that mostly grows wild around us. Fruit shape is round, yellow when riped with sweet and sour taste. There are many chemical properties have been attributed to cutleaf groundcherry including physalins B, E, G, H and I, withangulatin, withaferin, phytosterol, chlorogenic acid, flavonoids, saponins, citric acid, polyphenols, malic acid, alkaloids, tannins, cryptoxa...

  11. Perbandingan Pemberian Metoprolol Tartrat dengan Lidokain secara Intravena terhadap Perubahan Tekanan Darah dan Laju Nadi Akibat Tindakan Laringoskopi dan Intubasi

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    Yovita Koswara

    2014-08-01

    Full Text Available Laryngoscopy and endotracheal intubation associated with a sudden rise in blood pressure and pulse rate due to stimulation of sympathetic activity. The aim of this study was to compare effectiveness of metoprolol 5 mg intavenously and lidocaine 1.5 mg/kgBW to attenuate hemodynamic response evoked by laryngoscopy and intubation. This was an experimental randomized double blind controlled trial study was conducted in 40 patients with American Society of Anesthesiologist (ASA physical status I or II who will have surgery with general anesthesia techniques in Hasan Sadikin Hospital Bandung from July−August 2013. Subjects were divided into two groups wich received metoprolol 5 mg intravenously or lidocaine 1.5 mg/kgBW 3 minutes before laryngoscopy and intubation. All data were analysed using t-test, with p value < 0.05 considered significant. Statistical analysis showed that on second and third minutes after intubation and laryngoscopy showed a significant differences on hemodynamic parameter between two groups with p value <0.05. The conclusions of this study are intravenous 5 mg of metoprolol found to be better than lidocaine 1.5 mg/kgBW to attenuate hemodynamic response evoked by laryngoscopy and intubation.

  12. Penetapan Kadar Vitamin C Dari Paprika (Capsicum annum L. var Grossum) Secara Volumetri Dengan 2,6-Diklorofenol Indofenol

    OpenAIRE

    Siregar, Juni Arnita

    2014-01-01

    Chili pepper is a vegetable that is used in the needs of people's daily lives. Chili peppers are not only used for household consumption, but also a variety of processing industries like industrial consumption food and beverage, pharmaceuticals (drugs), cosmetics industry, and animal feed industries. Utilization as industrial raw materials to make chili peppers as a commodity of high economic value and have a clear business opportunity. The samples of chili peppers obtained from supermark...

  13. SELEKSI DAN PEMANFAATAN ACTINOMYCETES SEBAGAI MIKROBA ANTAGONIS YANG RAMAH LINGKUNGAN TERHADAP Fusarium oxysporum f.sp. cubense SECARA IN VITRO

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    I MADE SUDARMA

    2015-06-01

    Full Text Available A total of 119 different actinomycete isolate were recovered from banana crop habitats with and without Fusarium wilt disease symptom. These were than assessed for their antagonist ability against Fusarium oxysporum £sp. cubense (Foe in vitro. Results indicated that four of all actinomycete isolate active against Foe. The four of actinomycete isolates were Streptomyces sp. l (AAo4, Streptomyces sp.2 (AAo32 , Streptomyces sp.3 (AAo33 and Streptomyces sp. 4 (AAo35. It was can inhibit the Foe mycelium growth, 79,63%, 72,22%, 78,89% and 72,22% respectively. After tested with the 3 times replication, the four Streptomyces spp. isolate effective to control the Foe that attack Bali banana cultivars, such as Susu, Saba, Raja and Ketip.

  14. PENGARUH BAHAN PEMUTIH GIGI KARBAMID PEROKSIDA TERHADAP MUKOSA RONGGA MULUT SECARA MIKROSKOPIK (Penelitian pada Tikus Wistar Strain LMR

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    Harun Asyiq Gunawan

    2015-07-01

    Full Text Available Nowadays the use of over the counter (OTC bleaching material become more popular. The most common composition of this OTC material is mainly carbamide peroxyde and hydrogen peroxyde in carious concentration. There many methods for using carbamide peroxyde as bleaching material, such as spray, gel tray, or applicable solution, etc. According to the using methods, the possibility contamination between the material and oral mucous membrane is very high. The propose of this research is to examine the effect of 2% carbamide peroxyde solution on oral mucous membrane. Materials and method : 30 rats of wistar strain divided to 2 groups, the control groups consist of 10 rats, and experimental group of 20 rats. The animal of control received application on their labial vestibulum with aquadest, while for the experimental group applicated with 2% carbamide peroxyde solution. The applications were done for 1 minute, 3 times daily with 10 minutes interval periods. 5 rats of the control group and 10 of the experimental group were killed by epidural anaesthetic after 1 day application. The rest animals were killed after 3 days application. Inferior labia then taken as specimen, fixated using formaldehyde and processed for microscopic slides, stained with HE. Microscopic analyze were done using modified inflammation scoring system developed by Eda & Fukuyama. Statistical analyze shows that there are significant differences of the experimental group inflammation between 1 day and 3 days application. However there are no differences of oral mucous inflammation between 1 day and 3 days application for both control and experimental groups. Based on this research's result, it can be concluded that bleaching material contain carbamide peroxyde can caused oral mucous inflammation.

  15. Obstrüktif uyku apne sendromu semptom ve bulguları ile obezite arasındaki ilişki

    OpenAIRE

    Ünlü, Murat; İriz, Ayşe; Doğan, Berçem Ayçiçek; Dinç, A. Seçil Kayalı; Dursun, Engin; Eryılmaz, Adil; ACAR, Aydın

    2014-01-01

    Objective: To evaluate the effect of obesity on obstructive sleep apnea syndrome (OSAS) by means of objective and subjective data. Methods: A total of 70 patients were divided into obese (n=38; BMI>35 kg/m2) and non-obese patient groups (n=32; BMI Results: Obese patients had an average Mallampati score of Class III while the non-obese study participants had...

  16. Utilização da cefalometria como meio auxiliar no diagnóstico da síndrome da apnéia obstrutiva do sono

    OpenAIRE

    Bianca Rodrigues Casagrande; Rosalina Gonzales Ribas; Sérgio Billodre Trevisol

    2004-01-01

    Although the Sleep Obstructive Apnea Syndrome (OSA) was described about three decades ago in literature, only recently it got the attention of dentistry surgeons. Therefore the literature about the interrelation between the cephalometric analysis and pacients suffering from this disease has been revised. The review of literature showed meaningful differences between a series of the upper airway characteristics and the skeletal dimensions between OSA pacients and normal subjects. It h...

  17. Utilização da cefalometria como meio auxiliar no diagnóstico da síndrome da apnéia obstrutiva do sono

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    Bianca Rodrigues Casagrande

    2004-01-01

    Full Text Available Although the Sleep Obstructive Apnea Syndrome (OSA was described about three decades ago in literature, only recently it got the attention of dentistry surgeons. Therefore the literature about the interrelation between the cephalometric analysis and pacients suffering from this disease has been revised. The review of literature showed meaningful differences between a series of the upper airway characteristics and the skeletal dimensions between OSA pacients and normal subjects. It has been observed that the cephalometric analysis has been used as a major auxiliary means of diagnosis and in the planning of Sleep Obstructive Apnea Syndrome treatment associating it with a large number of variables in the prevalence of this Syndrome

  18. HARMONI KEHIDUPAN SOSIAL BEDA AGAMA DAN ALIRAN DI KUDUS

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    Moh Rosyid

    2015-11-01

    secara bersama-sama antara warga, tokoh, dan perangkat pemerintahan. Warga mayoritas tidak mudah tersulut pemberitaan media dan gosip yang tertebar setiap saat untuk menerkam yang minoritas. Terwujudnya toleransi beragama antar dan intern umat beragama dapat dilakukan dengan mengoptimalkan pendekatan budaya dan keharmonisan sosial. Dengan demikian, pendekatan yang menggunakan aspek keamanan secara alami ternafikan. Kata Kunci: Nirkonflik, Antar Umat dan Intern Umat Beragama, Aliran Agama. HARMONY OF SOCIAL LIFE OF DIFFERENT RELIGION IN KUDUS. Internal and interreligious conflict has colored social reality in this country. The most important thing to solve the problem is finding its fundamental cause. However, religious life among the society does not necessarily mean conflict or religious differences is not identical with conflict. Research conducted 2012 in Colo Village, Dawe, Kudus, Central Java shows that people from different religious background has created harmony among themselves. This research uses observation and interview to collect data from the research field and applies descriptive qualitative analysis. In 2012 the population in Colo is 4.327 people consisting of 2.400 family in which the majority are Moslem. There are also 16 Christians, 40 Buddhists and 14 Ahmadiyahs family living in one village. All religious adherents build their own sacred places consisting of five mosques (the Muria Mosque in Muria Mountain built by the Central Java Provincial government, two mosques in Colo village and a mosque built by Ahmady people, 11 mushalla (Muslim prayer house spread in four hamlet of Colo, a church beside a vihara. The vihara was built during Gus Durs presidency. In addition, there are two private Islamic schools, two elementary schools, a private junior Islamic high school (MTs, a private senior Islamic high school (MA, three Quranic learning centers, a kindergarten and an Islamic playgroup. Religious harmony among the society in Colo is established through

  19. Adiponectin provides cardiovascular protection in metabolic syndrome.

    Science.gov (United States)

    Okamoto, Yoshihisa

    2011-01-23

    Adipose tissue plays a central role in the pathogenesis of metabolic syndrome. Adiponectin (APN) is a bioactive adipocytokine secreted from adipocytes. Low plasma APN levels (hypoadiponectinemia) are observed among obese individuals and in those with related disorders such as diabetes, hypertension, and dyslipidemia. APN ameliorates such disorders. Hypoadiponectinemia is also associated with major cardiovascular diseases including atherosclerosis and cardiac hypertrophy. Accumulating evidence indicates that APN directly interacts with cardiovascular tissue and prevents cardiovascular pathology. Increasing plasma APN or enhancing APN signal transduction may be an ideal strategy to prevent and treat the cardiovascular diseases associated with metabolic syndrome. However, further studies are required to uncover the precise biological actions of APN.

  20. PENGUJIAN SIFAT PREBIOTIK DAN SINBIOTIK PRODUK OLAHAN UBI JALAR SECARA IN VIVO [In Vivo Evaluation of Prebiotic and Synbiotic Properties of Processed Sweet Potato Products

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    Lilis Nuraida1,2

    2008-12-01

    Full Text Available The aims of this research were to investigate the prebiotic and synbiotic properties of sweet potato products (combined with L. casei subsp Rhamnosus for probiotic in increasing the number of Lactic Acid Bacteria (LAB and suppressing the number of E. coli and the occurrence of Salmonella in vivo. Some previous study showed that sweet potato is a potent source of prebiotic. The sweet potato products evaluated were sweet potato flakes (SPF and sweet potato ice cream mix. The in vivo assay used male rat strain Sprague-Dawley. Total microbes, LAB, E. coli and Salmonella in fecal were analyzed before, during and after feeding period. Feeding with SPF as prebiotic, L. casei subsp Rhamnosus as probiotic, and combination of both as synbiotic for ten days were able to increase the number of LAB (0.4-1.1 log CFU/g and suppress the number of E. coli in rat feces (1.5-1.7 log CFU/g. All of the treatment did not affect the occurrence of Salmonella in rat feces. The treatment of sweet potato ice cream mix as prebiotics and the combination of sweet potato ice cream mix and L. casei subsp. Rhamnosus as synbiotic for ten days did not effect the number of LAB and E. coli in rat feces.

  1. Penyebaran Penyakit Rabies pada Hewan Secara Spasial di Bali pada Tahun 2008-2011 (THE SPATIAL DISTRIBUTION OF RABID ANIMAL IN BALI DURING 2008-2011

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    I Wayan Batan

    2014-08-01

    Full Text Available Rabies is a new emerging disease in Bali. The first rabies case was reported in October 2008 atKedonganan and Jimbaran village, District of South Kuta, Badung regency. The rabies diseasehas now been distributed to all areas of Bali causing death of more than a hundred of human beingand thousands of dogs. The aim of the studies was to make a map of rabies distribution in Balibased on cases in animals (dog and Bali cattle and human. The research was conducted bymaking a field surveys in nine regency of Bali. The surveys were focused on the occurrence ofhuman and animal rabies at the village level. The open ended questionaire was used in this surveyto collect data, and the secondary data also used in this study. Based on rabies disease mapping,it showed that the rabies have been distributed to eight regency and one city, covered 281 villagesout of 722 villages in Bali. In conclusion, the rabies had been distributed to all parts of Bali in threeyears periods.

  2. Ekspresi level gen mRNA protein ekstraseluler otak embrio mencit black-6 uk-12 akibat induksi 2-methoxyethanol : analisis secara real time RT-PCR

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    Yulia Irnidayanti

    2012-02-01

    Full Text Available The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metaboliteson the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin,tenascin, vimentin, Neurofilamen, NCam between brain embrio treatment with 2-ME at gestation day 12 and Embryo control wereachieved. The Electroforesis DNA on brain Embryonic day 12 showed that there were expression of GAPDH (447bp, Fibronectin(462bp, NCAM (293 bp, Tenascin (416bp, Vimentin (327, Neurofilamen high (301bp, Neurofilamen medium (289bp, Neurofilamenlow (398bp. This Data not showed. The expression of level of mRNA for protein Vimentin at embryonic brain treatment at GD-12 is487 copies, meanwhile on the embryinoc brain control is 209 copies. This expression is tendency very higher than control. Anotherlevel of mRNA for protein fibronectin, NCAM, Tenascin, Neurofilament were tendency not differe between embryinoc brain treatmentsand control. Intermediate filaments, vimentin, is found in specific cell types in the developing and adult central nervous systems (CNS,particularly astrocytes. Recently, found that vimentin immunoreactivities were increased in astrocytes and/or macrophages in the spinalcords of rats with autoimmune inflammation. So that The higher level mRNA for protein vimentin caused by effect 2-methoxyethanol.Vimentin contribute to the repair of brain through the migration of activated cells and increased level vimentin at embryionic braintreatment with 2-ME.

  3. PEMAHAMAN MASYARAKAT KECAMATAN MERGANGSAN, GONDOKUSUMAN, UMBULHARJO DAN KOTAGEDE YOGYAKARTA TERKAIT ANTIBIOTIKA Studi Pendahuluan Dalam Pengembangan Materi dan Metode Edukasi Dengan Pendekatan Secara Kualitatif: Diskusi Kelompok Terarah (DKT

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    Titien Siwi Hartayu

    2016-04-01

    Full Text Available Abstract: Antibiotics will be used safely when it is used rationally, and rational use of antibiotics need an appropriately of knowledge. In accordance to achieve appropriately knowledge about antibiotics, it is important to identify the level of knowledge about antibiotics to improve an educational method and material about antibiotics. As a qualitative study using focus group discussion (FGD among residence of Four Sub-district in Yogyakarta City, e.g.: Mergangsan, Gondokusuman, Umbulhardjo and Kotagede. There was eight key person of each sub district involved in the study. The inclusion criteria are: Residence of one of the four sub-districts with age above 17 years old, willing to attend the activities. The exclusion criteria are: residence with Pharmacy education background, moving limited and mental disability. Results of the study show the characteristic demography of the participants are: 5 Female and 3 Male, age range from 40 to 52 years old, with educational level: 5 participants are high school graduated and 3 participants are University graduated. All of participants are key person of each sub district whose familiar with the residences and the environmental where they are living. All of participants mention that they do not know about antibiotics et all including resistance and other risks of irrational use of antibiotics. All of participants asking for complete information about antibiotics which simple, accessible and could be socialized through the routine activities in every sub district e.g.: PKK (mothers gathering. In conclusion, the level of participants' knowledge about antibiotics is still low, and need to be improved. Complete information about antibiotics in a simple form has to be developed as an educational material. Keywords: Focus Group Discussion, Antibiotics, Knowledge

  4. KARAKTERISASI DAUN MIANA (Plectranthus scutellarioides (L. Bth. DAN BUAH SIRIH (Piper betle L. SECARA FISIKO KIMIA DARI RAMUAN LOKAL ANTIMALARIA DAERAH SULAWESI UTARA

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    Vivi Lisdawati

    2012-10-01

    Full Text Available Multidrug-resistant Plasmodium falciparum strains are an increasing problem in endemic areas and are partly responsible for the worsening malaria situation around the world. New cheap and efftctive compounds active in combination with available drug in the field, such as indegenous traditional medicine, are urgently needed. The aim of this work was to characterize the simplitia and extracts of an indegenous traditional medicine which usually used in North Sulawesi. Since this indigenous traditional medicine has been largely used and well-tolerated in humans, secondary metabolites derivatives of the plants could be in the near future associated with already available drugs in order to delay the spread of P. falciparum resistance. Non polar, semi polar and polar extracts of P. scutellarioides folium and P. bette fruit are prepared of each plants part obtained, then evaluated by using chromatography and densitometry methods following identification.   Keywords: P. falciparum, antimalarial activity, P. scutellarioides folium, P. betle fruit, secondary metabolites

  5. Uji Daya Hambat Sabun Cair Cuci Tangan pada Restoran Waralaba di Kota Padang Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro

    OpenAIRE

    Anisha Fazlisia; Elizabeth Bahar; Yulistini ,

    2014-01-01

    AbstrakSabun cair cuci tangan terbukti dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Sebagian besar restoran waralaba di Kota Padang menyediakan sabun cair cuci tangan yang telah diencerkan. Proses pengenceran mengubah kemampuan sabun dalam menghambat pertumbuhan bakteri. Penelitian bertujuan untuk menguji daya hambat sabun cair cuci tangan pada restoran waralaba di Kota Padang terhadap pertumbuhan E. coli dan S. aureus. Sampel diambil dari empat restoran wa...

  6. Karakterisasi Staphylococcus aureus Isolat Susu Sapi Perah Berdasar Keberadaan Protein-A pada Media Serum Soft Agar terhadap Aktivitas Fagositosis Secara In Vitro

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    Fajar Budi Lestari

    2017-02-01

    kompak dan 7 isolat (36,84% koloni berbentuk difus. Koloni kompak menunjukkan bakteri tersebut memiliki protein-A, koloni difus menunjukkan bakteri tersebut tidak memiliki protein-A atau memiliki protein-A tetapi tertutup oleh kapsul. Hasil uji fagositosis menunjukkan S. aureus yang memiliki protein-A lebih sedikit difagosit oleh leukosit polimorfonuklear (2,99 bakteri/sel dari pada S. aureus yang tidak memiliki protein-A, atau mempunyai protein-A tetapi tertutup oleh kapsul (3,85 bakteri/sel. Staphylococcus aureus yang memiliki protein-A lebih patogen daripada S. aureus yang tidak memiliki protein-A. Isolat S. aureus asal Jawa Tengah lebih virulen  dibandingkan isolat S. aureus asal Jawa Barat ditinjau dari sifat hemolisis, koagulase, dan  protein-A.

  7. Pelacakan Secara Imunohistokimiawi Antigen Virus pada Ayam yang Diinfeksi dengan Virus Penyakit Tetelo (IMMUNOHISTOCHEMICAL DETECTION OF VIRAL ANTIGEN IN TISSUE OF CHICKENS EXPERIMENTALLY INFECTED WITH NEWCASTLE DISEASE VIRUS

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    Anak Agung Ayu Mirah Adi

    2013-07-01

    Full Text Available In order to study the distribution of Newcastle disease virus (NDV following infection, chickenswere experimentally infected with visceretropic velogenic NDV isolate. Monoclonal antibodies (mAbsagainst the NDV LaSota vaccine strain were then produced to detect viral antigen in the infectedorgans. The mAbs were firstly tested for their specificity by enzyme linked immunosorbent assay(ELISA using NDV and normal allantoic fluids as antigens. Eight mAbs specific against NDVwere isolated and two mAbs were used for immunodetection of NDV antigen in chicken’s tissues.By immunohistochemistry labeled streptavidin-biotin (LSAB staining NDV–antigen was detectedin paraffin embedded tissues of NDV-infected chickens. NDV antigen was not detected in noninfected chickens. In the infected chickens, high intensity of NDV antigen was detected in thelymphoid tissues, lung and intestine. The NDV antigen with a lesser intensity was detected in thebrain, trachea, liver and myocardium. This study shows that although viscerotropic velogenicNDV isolate can infect almost all organs, the main target of infection are lung, intestine andlymphoids tissues

  8. ZONASI PERIKANAN PASI UNTUK KEPENTINGAN PEMANFAATAN SECARA BERKELANJUTAN SUMBERDAYA IKAN KAKAP MERAH DI KEPULAUAN LEASE (Pasi Zone for Interest Sustainable Utilization of Red Snapper Resources in Lease Islands

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    Delly Dominggas Paulina Matrutty

    2015-01-01

    Full Text Available ABSTRAK Pasi adalah daerah penangkapan spesifik ikan kakap merah di Kepulauan Lease. Eksploitasi terhadap sumberdaya ikan kakap merah cenderung tinggi akhir-akhir ini karena sangat disukai di pasar lokal, regional maupun internasional, selain dijadikan sebagai objek wisata pancing. Kondisi ini akan mengancam kelestarian sumberdaya jika tidak dikelola dengan baik. Penetapan zonasi merupakan salah satu alternatif pengelolaan sumberdaya perikanan yang baik, dan jika dilakukan dengan benar akan memberikan nilai tambah bagi masyarakat, tanpa mengganggu kelestariannya. Tujuan penelitian ini adalah menentukan zonasi perikanan pasi untuk kepentingan pemanfaatan berkelanjutan sumberdaya ikan kakap merah di Kepulauan Lease. Data yang digunakan meliputi hasil kajian potensi sumberdaya ikan kakap merah, kondisi oseanografi  daerah penangkapan (pasi, dan sistem nilai perikanan pasi yang meliputi komponen nilai dasar ekologi, sosial dan teknologi, serta metode Participatory Rural Appraisal (PRA.  Berdasarkan kriteria yang dibangun dari seluruh aspek tersebut  maka dibuat zonasi khusus untuk kawasan pasi di Kepulauan Lease.  Berdasarkan hasil  analisis  diperoleh 4 (empat dari  25  pasi  ditetapkan sebagai zona lindung, 21 pasi ditetapkan sebagai zona perikanan berkelanjutan sub-zona perikanan tangkap, sedangkan 13 di antaranya ditetapkan sebagai zona pemanfaatan sub-zona wisata pancing. ABSTRACT Pasi is a specific fishing ground for Red Snapper in Lease Islands.  Recently, the exploitation of the red snapper population is tend to increase due to high demand of local, regional and international market as well as object for fishing tourism. This condition will threat the sustainability of the resources as if it is unmanaged in good way.  Zone determination is one of the alternatives of good fisheries resources management. If it is done in a right way, it will add value to community without disturbing the sustainability. The objectives of the present study were to determine fisheries pasi zone to the sustainability importance of red snapper resources in Lease Islands.  Data used included study of potential of red snapper resources, oceanographically condition, fishing ground (pasi, and fisheries value system namely basic value of ecological component, social and technology as well as PRA method (Participatory Rural Appraisal.  Based on the criteria which were formulated of all aspects, specific zonation was made for pasi region in Lease Islands.  The analysis showed that there were 4  out of 25 pasi determined as shelter zone, 21 pasi was for sustainable fisheries zone sub-zone of catch whereas 13 pasi was determined as utilization zone sub-zone fishing tourism.

  9. Uji Daya Hambat Sabun Cair Cuci Tangan pada Restoran Waralaba di Kota Padang Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro

    Directory of Open Access Journals (Sweden)

    Anisha Fazlisia

    2014-09-01

    Full Text Available AbstrakSabun cair cuci tangan terbukti dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Sebagian besar restoran waralaba di Kota Padang menyediakan sabun cair cuci tangan yang telah diencerkan. Proses pengenceran mengubah kemampuan sabun dalam menghambat pertumbuhan bakteri. Penelitian bertujuan untuk menguji daya hambat sabun cair cuci tangan pada restoran waralaba di Kota Padang terhadap pertumbuhan E. coli dan S. aureus. Sampel diambil dari empat restoran waralaba dan diuji dengan metode difusi. Hasil penelitian menunjukkan bahwa keempat sabun dapat menghambat pertumbuhan S. aureus namun hanya sebagian yang memiliki daya hambat terhadap E. coli. Median daya hambat pertumbuhan E. coli dan S. aureus pada setiap periode yaitu 0, 7.4, 7.3 dan 0, 26.1, 23.3. Nilai maksimum daya hambat pertumbuhan E.coli dalam tiga periode yaitu 19.5, 35.4, 27.1 dan 20.7, 40.2, 36.6 untukS. aureus. Daya hambat minimum terhadap kedua bakteri adalah 0.00. Hal tersebut dapat dipengaruhi komposisi dan konsentrasi antiseptik, antibakteri, pH sabun, pengenceran dan struktur dinding sel bakteri. Disimpulkan bahwa sabun cair cuci tangan yang diuji memiliki kemampuan lebih besar dalam menghambat pertumbuhan S.aureus daripada E.coli.Kata kunci: sabun cair cuci tangan, restoran, pengenceran, uji daya hambat, pertumbuhan bakteriAbstractLiquid hand soaps proved to inhibit Escherichia coli and Staphylococcus aureus bacterial growth. In Padang, most of Restaurants provide diluted liquid hand soaps. Research found dilution changed soap ability to inhibit bacterial growth. The purpose of this study was to examine the ability of Padang City Restaurants’ liquid hand soaps to inhibit E. coli and S. aureus bacterial growth. The samples were taken from four restaurants and examined by using diffusion method. The results showed all of them could inhibit S. aureus but only a half inhibited Escherichia coli bacterial growth. Median for E. coli and S. aureus bacterial inhibition growth for each period were 0, 7.4, 7.3 and 0, 26.1, 23.3. Maximum inhibition value for E. coli growth in the first, second, and third periods were 19.5, 35.4, 27.1 and 20.7, 40.2, and 36.6 for S. aureus. In addition, minimum inhibition in both bacteria were 0.00. It could be influenced by soap antiseptic and antibacterial composition and concentration, pH, dilution, and structure of bacterial cell wall. In conclusion, the liquid hand soaps has greater ability to inhibit S. aureus than E. coli.Keywords: liquid hand soaps, restaurant, dilution, inhibitory test, bacterial growth

  10. Karakterisasi Simplisia Dan Isolasi Serta Analisis Komponen Kimia Minyak Atsiri Dari Buah Jintan Putih (Cuminum cyminum L.) Secara GC-MS

    OpenAIRE

    2011-01-01

    One of the essential oil-producing plants and had been used for a long time by Indonesian society as a efficacious medicinal plants is Cuminum cyminum L. Cuminum cyminum L. is a widely used ingredient. It has been used for a very long time in traditional medicine. The quantitative determination of volatile oil was accomplished by Stahl apparatus and the isolation of volatile oil was accomplished by water distilation and steam distillation. The components of volatile oil was analyzed by Ga...

  11. Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED

    Directory of Open Access Journals (Sweden)

    Ni Wayan Sudatri

    2015-05-01

    Full Text Available In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L. Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad, P2 (2x200 rad, P3 (3x200 rad andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05 between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.

  12. Korelasi indeks morfologi wajah dengan sudut interinsisal dan tinggi wajah secara sefalometri (Cephalometric correlation of facial morphology index with interincisal angle and facial height

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    Pricillia Priska Sianita K

    2013-12-01

    Full Text Available Background: In a disaster or criminal case, comprehensive information is needed for identification process of each victim. Especially for some cases that only leave skull without any information that could help the identification process, including face reconstruction that will be needed. One way of identifications is specific face characteristic, race, some head-neck measurements, such as facial morphology index, interincisal angle and facial height. Purpose: The aim of study was to determine the correlation of facial morphology index with interincisal angle and facial height through cephalometric measurement. Methods: The samples were cephalogram of 31 subjects (Deutro-Malayid race who met the inclusive criteria. Cephalometric analysis were done to all samples and followed by Pearson Correlation statistical test. Results: The correlation was found between facial morphology index and facial height, but no correlation between facial morphology index and interincisal angle. Conclusion: The study showed that the cephalometric measurement of facial morphology index and facial height could be used as the additional information for identification process.Latar belakang: Dalam bencana alam atau kasus kriminal informasi yang komprehensif diperlukan untuk proses identifikasi masing korban. Khususnya pada beberapa kasus yang hanya meninggalkan tengkorak tanpa informasi yang dapat membantu proses identifikasi, termasuk rekonstruksi wajah yang akan dibutuhkan. Salah satu cara identifikasi karakteristik wajah tertentu, ras, beberapa pengukuran kepala leher, seperti indeks morfologi wajah, sudut interincisal dan tinggi wajah. Tujuan: Penelitian ini bertujuan meneliti korelasi indeks morfologi wajah dengan sudut interincisal dan tinggi wajah melalui pengukuran sefalometrik. Metode: Sampel penelitian adalah cephalogram dari 31 subyek ras Deutro - Malayid ras yang memenuhi kriteria inklusif. Analisis cephalometri dilakukan pada semua sampel dan dilanjutkan dengan uji statistik korelasi Pearson. Hasil: Korelasi ditemukan antara indeks morfologi wajah dan tinggi wajah, tapi tidak ada korelasi antara indeks morfologi wajah dan sudut interincisal. Simpulan: Hasil penelitian menunjukkan bahwa pengukuran sefalometrik indeks morfologi wajah dan tinggi wajah, dapat digunakan sebagai informasi tambahan untuk proses identifikasi.

  13. Ketahanan Hidup Sel Acetobacter xylinum pada Pengawetan secara Kering-Beku Menggunakan Medium Pembawa (Viability of A. xylinum Subjected to Freeze Drying Using Carrier Media

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    Noor Aini Habibah

    2009-03-01

    Full Text Available A research on the use of sucrose and lactose as carrier media to protect Acetobacter xylinum cell subjected to freeze drying has been done. The aim of the research was to know the number of the viable cells from dried culture and to know the concentration of the carrier medium that would give best result. The best result is sucrose at the concentration of 15% that produced 28.2 x 106 viable cells/ ml of rehidrated culture. The rehidrated culture used in the research was Schramm & Herstin medium. Key words : Acetobacter xylinum, freeze drying, carrier media

  14. ANALISA FLAVONOID DARI EKSTRAK ETANOL 96% KULIT BUAH OKRA MERAH (Abelmoschus esculentus L. Moench SECARA KROMATOGRAFI LAPIS TIPIS DAN SPEKTROFOTOMETRI UV-VIS

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    Nia Lisnawati

    2016-03-01

    Full Text Available Has done research on flavonoids Analysis of Ethanol Extract 96% Fruit Leather Red Okra In Thin Layer Chromatography and Spectrophotometer UV-Vis. The purpose of this study was to analyze the content of the fruit skin red okra (Abelmoschus esculentus L. Moench by using the method of thin layer chromatography (TLC under UV light and spectrophotometry UV-Vis. Reference standards used in this study is the Standard Solution Routine Quercetin. The results of the research that has been done by the method of thin layer chromatography obtained Rf values of 0.81 and produces the color orange. And the results of research conducted by spectrophotometry UV-Vis method obtained 333,117 mg.L-1 or 421,629 mg.kg-1 or 0,84339 %. The conclusion from this study is that the 96% ethanol extract of the fruit leather red okra (Abelmoschus esculentus L. Moench positive (+ contains flavonoids with levels of 0,84339 %.

  15. Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG

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    Yuli Purwandari Kristianingrum

    2016-01-01

    Full Text Available Infectious Bovine Rhinotracheitis (IBR is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.

  16. Validasi Metode Analisis Ofloksasin dalam Plasma In Vitro secara Kromatografi Cair Kinerja Tinggi-Fluoresensi Mengacu pada European Medicines Agency Guideline

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    Letitia Tania

    2016-08-01

    Full Text Available Ofloxacin is an antibiotic from second generation of fluoroquinolone's group. Concentration of ofloxacin in human plasma is low, so it requires a selective, accurate, sensitive method for analysis it. In this study, the optimization and validation of ofloxacin analysis in human plasma using high performance liquid chromatography-fluorescence with ciprofloxacin-HCl as an internal standard were carried out. Separation of ofloxacin was performed using C18 (Waters, Sunfire TM 5 µm; 250 x 4.6 mm column with an isocratic mobile phase consisted of triethylamine 1% on water pH 3.0–acetonitrile (84:16 an in the flow rate of 1.0 mL/min, and on column temperature 40oC whereas the detection was carried out at excitation of 300 nm and emission of 500 nm. Plasma extraction was done by deproteination using methanol, through the process of vortex and centrifugation (10000 rpm for 2 minutes and 10 minutes consecutively. The method was valid and linear within the concentration ranged from 21,4 ng/mL to 4280 ng/mL with LLOQ of 21,4 ng/mL. Intra-day and inter-day accuracy and presicion was not more than + 20% for LLOQ and not more than + 15% for QCL, QCM, and QCH samples in both % diff and coefficient of variation. Ofloxacin was stable in human plasma at least three freeze and thaw cycle, for at least 24 hours in room temperature and 28 days at -20oC. This bioanalytical method fulfilled the acceptance criteria following EMEA guideline.

  17. PENGHAMBATAN CAJUPUTS CANDY TERHADAP VIABILITAS KHAMIR Candida albicans SECARA IN VITRO [Inhibition of Cajuputs Candy Toward the Viability of Candida albicans by using In Vitro Assay

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    C. Hanny Wijaya1*

    2014-12-01

    Full Text Available The utilization of cajuput essential oil as a flavor in candy may produce a physiological active added value. Some compounds of cajuput plant (Melaleuca cajuputi L have been reported for their anti-microbial activities. Candida albicans is a normal commensal organism in human mouth. However, it may become virulent and responsible for oral diseases known as oral candidiasis. This study aimed to determine the effect of cajuput and peppermint oil in cajuputs candy in inhibiting the C. albicans biofilms formation by using in vitro biofilm assay and viability assay. Furthermore, the influence of concentration of cajuput oil on the anti-microbial activities had been analyzed. All the tested concentration of cajuput oil in cajuputs candy was effective to inhibit the viability of C. albicans. The provision of flavor components of cajuput and peppermint oil could produce synergistic effects compared to a single flavor component. The addition of cajuput oil at 0.6% was able to inhibit the viability of C. albicans. The activities of the cajuput oil showed positive correlation to the concentration. The variable of plus and minus 0.1% addition of the cajuput oil concentration, however, produced no significant difference to inhibit the growth of C. albicans in biofilm. Sensory test, hedonic test, was conducted to evaluate the flavor, aroma, and overall attributes, resulting in no significant difference between 0.6 to 0.8% additions of cajuput oil upon the sensory acceptance.

  18. Strategi Pengelolaan Usaha Jasa Rumah Pemotongan Hewan Ruminansia Secara Berkelanjutan (MANAGEMENT STRATEGY FOR SUSTAINABLE RUMINANT-CATTLE SLAUGHTERHOUSE (RC-S SERVICES

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    Maya Dewi Dyah Maharani

    2017-04-01

    Full Text Available Uncontrolled cattle slaughtering may result in reduce water quantity and quality, number of cattle,and product of animal origin that does not fit the criteria of safety-healthy-whole-halal. The researchobjective was to analyze and measure important variables which might be requiredin developinga modelas well as to formulate strategic plans for managing sustainable abattoir services. Analysis methods usedin this study were: Multidimensional Scaling (MDS or rap-slaughterhouse, Monte Carlo Analysis, andAnalytical Hierarchy Process (AHP. Results of the analysis showed that the important variables were,i.e.: water availability and animal health status (ecological dimension; willingness to conduct hygienicand sanitation procedures at business scale level, service business orientation, availability of animal andslaughtering schedule management (economical dimension; workers safety and security (sociologicaldimension, noncompliance of the use of infrastructure by managers, workers and service users; lack offulfillment of the requirements of the building frame (dimension regulations; and processing technology(technological dimension.The priority strategic analysis concluded that supply services of product ofanimal origin is eligible to be considered as components required in the food security program through thedevelopment and renovation program of effective slaughterhouses.

  19. PEMECAHAN DORMANSI DAN PERKECAMBAHAN ASAM KURANJI (Dialium indum L. SECARA MEKANIS DAN KIMIAWI Breaking Dormancy and Seeds Germination of Asam Kuranji (Dialium indum L. with Mechanical and Chemical

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    Bakti Nur Ismuhajaroh

    2016-08-01

    Full Text Available The purpose of the study was to find out the dormancy breaking and the seed germination of Asam kuranji (Dialium indum L. mechanically by sandpapering the seeds and chemically by immersing them in sulfuric acid (H2SO4. The experiment was conducted at the Plant Physiology Laboratory of Agricultural Faculty of Lambung Mangkurat University from June - August 2013 using Completely Randomized Design (CRD with 2 x 4 factorial. The first factor was the mechanical treatment  (s0 = without sandpaper and s1 = with sandpaper, the second factor was the chemical treatment (a0 = without immersion, a1 = 70% of H2SO4, a2 = 80% of H2SO4, and a3 = 90% of H2SO4. The data were analyzed by ANOVA at the level of 95% followed by Tukey's test at the level test of 95%. The results showed that the treatments of sandpapering and immersing the seeds significantly affected the % of germination, the length of radicle and the length of hypocotyl. The interaction between treatments of sandpapering and immersion indicated that the higher the concentration of H2SO4 immersion the higher the % of germination, the length of radicle and the length of hypocotyl. Keywords: germination, Dialium indum L., sandpaper, concentration, H2SO4

  20. PENGARUH PEMAKAIAN KOMBINASI MINYAK JINTEN HITAM (Nigella sativa dan MADU (Apis mellifera SEBAGAI ANTI-ACNE SECARA TOPIKAL PADA SUKARELAWAN WANITA

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    Baiq Lenysia Puspita Anjani

    2015-11-01

    Full Text Available Acne mostly affects teenagers with prevalence of 85%. Changing habits of cosmetics may affect the incidence of acne vulgaris. Healing acne can use herbal ingredients such as black cumin oil and honey. Black cumin oil effective for treating mild acne vulgaris and moderate acne vulgaris. Honey has property that can kill the bacteria Propionibacterium acnes. The purpose of this study is to determine the anti-acne activity in use of black cumin oil and honey combination in 1:1 ratio for 14 days in women. This study included experimental study with pre-post treatment study design. There are 2 groups, control group (six women volunteer and treatment group (six women volunteer. For 14 days, control group use chemical drugs anti-acne, while treatment group use black cumin oil and honey combination in 1:1 ratio. Analysis of data used independent sample t-test with confidence interval 95%. The results show that treatment group of women who use black cumin oil and honey combination in 1:1 ratio for 14 days compared with the control group of women there is no difference in reducing the number of parameters black blackheads, white blackheads, papules, pustules, hyperpigmentation, erythema, pruritis, xerosis, desquamation, and sunburn with a value of p> 0.05. The conclusion of this study is the use black cumin oil and honey combination in 1:1 ratio for 14 days has effect as anti-acne in women, but statistically not significant.

  1. Pengaruh Tes Elevasi Tungkai Secara Pasif terhadap Variasi Pletismograf untuk Penilaian Responsivitas Cairan pada Pasien yang Dilakukan Pembedahan dengan Anestesi Umum

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    Bahtiar Susanto

    2016-08-01

    Full Text Available Fluid responsiveness assessments have shown to be an important matter in perioperative fluid optimization. Respiratory variations in pulse oximetry plethysmographic waveform amplitude (∆POP and passive leg raising have been shown as promising indicators due to the ability to predict fluid responsiveness. The aim of this study was to assess the effect of passive leg raising (PLR on ∆POP to predict fluid responsiveness in mechanically ventilated patients after induction of general anesthesia. This was a trialon 30 patients referred for surgery under general anesthesia, aged 18–60 years and ASA physical status I or II, during the period of February–March 2015 at the Central Surgical Installation of Dr. Hasan Sadikin General Hospital Bandung. Patients were studied immediately after the induction of general anesthesia. This trial use the paired t test, Wilcoxon test, and ANOVA for statistical analysis. Vital signs and ∆POP were recorded at baseline, before, and after PLR. PLR induced changes in ∆POP with a ∆POP greater than 13% compared to the initial PLR allowed discrimination between responders and nonresponders to 6/30. There was a significant decrease in ∆POP in responders when compared to the nonresponders(p<0.05. ∆POP can be reduced by PLR and fluid responsiveness can be predicted noninvasively in mechanically ventilated patients during general anesthesia.

  2. Penetapan Kadar Kalsium pada Daun katuk (Sauropus androgynus (L.) Merr.) dari Daerah Karo dengan Daerah Pematang Johar Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Manik, Nova Driyanti

    2011-01-01

    Katuk leaf’s (Sauropus androgynus (L.) Merr.) constituting consumed vegetable among society. Besides it’s relative cheap price and easy at gets, this katuk leaf’s is good for health because contain high enough nutrient. In a life every day, people consume katuk leaf’s to fluent ASI production, estimate because level of calcium high enough that complete necessity the baby bone. The purpose of this study was to determine the level of calcium that variably between region of Karo with region o...

  3. AKTIVITAS ANTIOKSIDAN MINYAK BEKATUL PADI AWET DAN FRAKSINYA SECARA IN VITRO [In Vitro Antioxidant Activity of Stabilized Rice Bran and Its Fraction

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    Evy Damayanti1

    2004-04-01

    Full Text Available Some Researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in the prevention of low density lipoprotein (LDL and human lymphocyte from oxidation under oxidative stress was still limited. The objective of this study was the investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LCL and lymphocyte from oxidation.Human plasma were supplemented with the samples of : rice bran oil (RBO, unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308.3, 22.2, 5.2, 10.4, and 10.4 µg/ml, respectively. Afterward, the human LDL were collected by ultracentrifuge and diluted until a concentration of 200 µg protein/ml was reached. Human LDL isolates were then oxidized with CuSO4 5 µM for measuring antioxidant activity of the sample. The length of incubation, H2O2 concentration, period of sample supplemented into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured. The samples used in the lymphocyte were RBO IR-64, unsaponifiable matter IR-64, and oryzanol standard at the concentrations of 133.2 – 2, 132.0 µg/ml, 9.6 – 153.6 µg/ml, and 2.4 – 37.7 µg/ml, consecutively.The result showed that malonaldehyde concentration in human LDL decreased significantly (α = 0.05, 15 – 41% and 39 – 56% compared to the control. The absorbance of living lymphocyte cell in culture was not influenced by the type and concentration of RBO and its fraction. The addition of hydrogen peroxide (H2O2 3 mM into culture sifnificantly lowered the absorbance as compared to culture without (H2O2

  4. UJI VIABILITAS SERBUK SARI SECARA In-Vitro KELAPA (Cocos nucifera L.“Rangda” DENGAN WAKTU DAN SUHU PENYIMPANAN YANG BERBEDA

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    Sista Nirmala

    2013-12-01

    Full Text Available This aims of the research was to determine the structure and pollen viability of RangdaCoconut (Cocos nucifera L. “Rangda” stored at different time and temperatures. Pollen sampleswere collected from Gianyar, Klungkung and Negara. The experiment was conducted at theLaboratorium of Forensic and the Laboratorium of Plant Development and Structure, UdayanaUniversity, from October 2012 to January 2013. Pollen structure was analyzed with acetolysemethod, while pollen viability was analyzed in-vitro. The results showed that the structure of thepollen of “Rangda” coconut was circular, media, monosulcate, Subferoidal-Prolat, Sferoidal.Pollen viability of “Rangda" coconut was low (less than 3%. Pollen stored in the freezer has thebest viability, which continually increased until the 2nd weeks, then declined after that, whiepollen viability stored at room temperature tend to decline up to the fourth weeks.Keywords: structure, viability, pollen, time, storage

  5. Efek Relaksasi Ekstrak Etanol Daun Pugun Tanoh (Curanga fel-terrae (Lour.) Merr.) Terhadap Kontraksi Otot Polos Ileum Marmut (Cavia porcellus) Terisolasi Secara In Vitro

    OpenAIRE

    Michi, Anddora

    2015-01-01

    Pugun Tanoh (Curanga fel-terrae (Lour.) Merr.) is a plant of Scropulariaceae family which is often used by the native people of Tiga Lingga village in Dairi Regency, North Sumatera Province, Indonesia to cure diarrhea and stomachache. This research aims to observe the relaxation effect of Pugun Tanoh leaf ethanol extract on the contraction of isolated guinea pig’s ileum by acetylcholine. This research was conducted using in vitro. The parameter measured in this research is the contract...

  6. ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI GAGAL LELANG PENGADAAN BARANG DAN JASA PEMERINTAH SECARA ELEKTRONIK ( E-PROCUREMENT DI KABUPATEN BADUNG

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    I N. R. Karyasa

    2014-02-01

    Full Text Available Starting in 2012 implementation of the procurement of goods / services in Badung regency have been conducted electronically (e-Procurement by E-tendering procedure. But implementation in construction work package there were many failed auctions. This study aims to find out what factors that affect occurance of failed auction and what dominant factors influence it. The sample used in this study were 50 contractor company by using non-probability sampling with purposive sampling and data collection by using questionnaires. Data analysis was performed with factor analysis.   The results shows there are 5 factors that have affect to the occurrence of failed auctions, the procurement with cumulative variance 84.327% that from 30 variable. The most dominant factors is I factor with eigenvalues 7.818 and variance 26.061% that formed by 8 variables. The eight variables compiler of I factor that are, not yet ready with the procurement of goods /service electronically, fail to upload offering documents, the lack of time availability to prepare offering documents, late to know auction announcement, personnel qualifications of project team do not match, ther is no / in-appropriate support from suppliers, incomplete offering documents and do not ready with evidence of qualifications.

  7. PENENTUAN Streptococcus Group A PENYEBAB FARINGITIS PADA ANAK MENGGUNAKAN McIsaac SCORE DAN RAPID ANTIGEN DETECTION TEST (RADT DALAM UPAYA PENGGUNAAN ANTIBIOTIKA SECARA BIJAK

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    AA Agustia Sinta Dewi

    2014-03-01

    Full Text Available Pharyngitis can be caused by viruses and bacteria. The bacteria that most commonly causes pharyngitis is Streptococcus Group A. In the treatment of pharyngitis, it is very important to ensure the cause for determining the appropriate treatments, therefore unnecessary use of antibiotics can be avoided. Antibiotics should be prescribed in patients with pharyngitis caused by bacteria. Diagnostic test that can be applied to determine the causes of pharyngitis are McIsaac score and Rapid Antigen Detection Test (RADT. The purpose of this study was to investigate the presence of Streptococcus Group A as the cause of pharyngitis applying McIsaac scores and the RADT. This study was cross-sectional. Patients with the inclusion and exclusion criteria were given an initial assessment using the McIsaac score, subsequently tested with the RADT. The results gained from the McIsaac scores and subsequent RADT were compared.  It was found that as many as 124 patients suspected of having bacterial pharyngitis. Forty two of them were scored 3; 55 patients scored 4, and  27 patients scored 5. All patients tested with the RADT, only 18 patients gave positive results. Out of those 18 patients positively tested, 6 patients scored 3; 8 patients scored 4, and 4 patients scored 5. In was concluded that the use of RADT was better than McIsaac scores in determining pharyngitis caused by Streptococcus Group A.

  8. Uji Daya Hambat Sabun Cair Cuci Tangan pada Restoran Waralaba di Kota Padang Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro

    National Research Council Canada - National Science Library

    Anisha Fazlisia; Elizabeth Bahar; Yulistini

    2014-01-01

    .... Proses pengenceran mengubah kemampuan sabun dalam menghambat pertumbuhan bakteri. Penelitian bertujuan untuk menguji daya hambat sabun cair cuci tangan pada restoran waralaba di Kota Padang terhadap pertumbuhan E. coli dan S. aureus...

  9. PEMBELAJARAN TATA BAHASA INGGRIS SECARA KOMUNIKATIF DENGAN PENYAJIAN INDUKTIF DAN PENGINTEGRASIAN KETERAMPILAN BERBAHASA: STUDI KASUS DI KELAS BAHASA INGGRIS I DI IAIN RADEN FATAH PALEMBANG

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    Annisa Astrid

    2011-11-01

    Full Text Available AbstractTeaching grammar has been regarded as prominence in teaching a language where there are some different views of how to teach it. Recently, with the introduction of Communicative Language Teaching, teaching grammar is emphasized on experiential learning and communicative goals. For these reasons the writer tried to implement inductive approach in Bahasa Inggris class, where all the students are from Non English Department. In inductive approach, the learners study examples and from these examples derives an understanding of the rule. the writer also presented the grammar integrated with four language skills (speaking, listening, reading, and writing, where the writer paid more attention to enabling students to work with the target language during the lesson and communicate in it by the end of it.. In totally 14 meetings, the teacher applied inductive approach in presenting the materials and gave all exercises integratedly to four language skills (speaking, writing, reading and listening. The data were collected by means of T-Test. From the results of the T-test, it can be seen that the T value is 5,193, with the significance value 0,001. T value is more than T table ,2,042 . It can be interpreted that there is a significant difference between the students’ achievement in grammar before the treatment and after the treatment by using inductive approach and applying the activities that integrates grammar into four language skills. It was also found that the students were enthusiastic along the teaching and learning processes by implementing inductive approach. Keywords: inductive, communicative language teaching

  10. HIDROLISIS IKAN BERNILAI EKONOMI RENDAH SECARA ENZIMATIS MENGGUNAKAN PROTEASE BIDURI [Enzymatic Hydrolysis of Low Economic Value Fishes using Biduri’s Protease

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    Yuli Witono1*

    2015-07-01

    Full Text Available Fish protein hydrolyzate is a product obtained from the decomposition of fish proteins into short-chain compounds due to the hydrolysis process either by enzymes, acids, or bases. The purpose of this study was to optimize the production of fish protein hydrolyzate from the low economic value fishes including 'bibisan' (Apogon albimaculosus, 'baji-baji' (Platycephalidae cymbacephalus, and the 'lidah' (Cynoglossus lingua obtained from Talango Island, Madura. Production of fish protein hydrolyzate was conducted using (Calotropis gigantea protease at various concentrations (0, 0.7, 1.4, and 2.1 Unit/g and at various hydrolysis times (0, 1.5, and 3 h. The experimental design was a Completely Randomized Design with two factors and the experiments were carried out in triplicates. The results showed that interactions between protease concentrations and hydrolysis times significantly affected (at 5% level test the soluble proteins, maillard products, and the level of rancidity measured as thiobarbituric acid (TBA. The best fish hydrolyzate product based on the soluble protein parameter was resulted from 2.1 unit/g of biduri’s protease with a hydrolysis time of 1.5 h. The hydrolyzate produced had 3.51% of soluble proteins, a maillard value of 0.63, and rancidity levels of 12.21 mmol TBA/kg.

  11. Penetapan Kadar Mineral Besi,Kalsium, Magnesium, dan Kalium pada Kacang Panjang (Vigna unguiculata (l) Walp.) Segar dan Rebus secara Spektrofotometri Serapan Atom

    OpenAIRE

    Simamora, Alex Leo

    2017-01-01

    Long beans (Vigna unguiculata (l). Walp) is one of the vegetables that contain minerals and vitamins is good for consumption. Parts can be consumed from this plans are fruits and young leaves. Both fruit and leaves quite a lot of nutrients that the body needs. Long beans contain vitamins and minerals, among which calcium, iron, magnesium, potassium, protein, fat, carbohydrates, phosphorus, calories, vitamin A, vitamin B and vitamin C. This study aims to determine the mineral content of iron, ...

  12. PENGUSAHAAN TERUMBU KARANG SECARA BERKELANJUTAN (Studi Kasus Perubahan Usaha Terumbu Karang Alam ke Budidaya Terumbu Karang di Pulau Serangan, Kccamatan Denpasar Selatan, Kota Denpasar-Bali

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    ARSONETRI ARSONETRI

    2015-05-01

    Full Text Available Illegal coral extraction (taking coral from nature as an unsustainable effort affected the degradation of coral reef ecosystem and coastal environment. However, 1t continually happens in Indones1a, particularly in Scr,rngan Island, Bali. The stakeholder in coral extraction effort have gamed good advantages (benefits making a legally and sustainable clTort such as coral culture still difficult. This study 1s specifically examines the methods of coral culture 1s better and profitable than the methods of coral extraction from nature. Thus, encouragmg all parlles involved (stakeholder, especially fishem1an to apply the coral culture. The research is observed and compared between coral extraction from nature fisherman and coral culture fisherman in Serangan Island, the feasibility, benefits and fisherman's perception. It is also explain the role of government toward of coral extraction and culture. 1he result shows the benefit or advantage of coral culture is higher than coral extraction from nature. It demonstrated by NPV (Net Present Value and CBR (Cost Benefit Ratio of coral culture is higher than coral extraction from nature. Therefore, coral culture is economically feasible to replace coral extraction from nature. The fisherman's perception is very pos1llve regarding coral culture effort and implementation replacing coral extraction from nature. In this effort, the government's role is very important in provision mcentive such as modal and subs1d1es, trainings and increasing fisherman capacity building, market insurance, and easier to obtain permits of coral culture.

  13. Gallbladder Wall Thickening and Periportal Tracking on CT in Patients with Acute Pyelonephritis

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Jae Shik; Sung, Deuk Jae; Park, Beom Jin; Kim, Min Ju; Cho, Sung Bum; Han, Na Yeon; Lee, Nam Joon [Dept. of Radiology, Anam Hospital, Korea University College of Medicine, Seoul (Korea, Republic of)

    2012-09-15

    To assess gallbladder (GB) wall thickening and periportal tracking on CT in patients with acute pyelonephritis (APN) by each kidney involvement. A total of 117 patients diagnosed with APN by clinical examination and CT were included in this retrospective study. The patients were divided into three groups based on kidney involvement seen on CT: right APN, left APN, and bilateral APN. Two radiologists reviewed CT images in consensus for the presence of diffuse GB wall thickening and periportal tracking. The prevalence of these two manifestations was compared among the three groups. The prevalence of GB wall thickening in patients with right APN (26.4%) and bilateral APN (19%) was significantly higher than in patients with left APN (0%) (p < 0.016). The prevalence of periportal tracking in patients with right APN (15.1%) and bilateral APN (26%) was higher than in patients with left APN (3%), with a significant difference between patients with bilateral APN and left APN (p < 0.016). GB wall thickening and periportal tracking are predominantly encountered on CT in patients with APN involving the right kidney (right and bilateral APN), and should be considered in differential diagnosis of diseases associated with GB wall thickening and periportal tracking.

  14. Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes

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    Moar William J

    2005-06-01

    Full Text Available Abstract Background Insecticidal toxins from Bacillus thuringiensis bind to receptors on midgut epithelial cells of susceptible insect larvae. Aminopeptidases N (APNs from several insect species have been shown to be putative receptors for these toxins. Here we report the cloning and expression analysis of four APN cDNAs from Spodoptera exigua. Results Suppression Subtractive Hybridization (SSH was used to construct cDNA libraries of genes that are up-and down-regulated in the midgut of last instar larvae of beet armyworm, S. exigua exposed to B. thuringiensis Cry1Ca toxin. Among the clones from the SSH libraries, cDNA fragments coding for two different APNs were obtained (APN2 and APN4. A similar procedure was employed to compare mRNA differences between susceptible and Cry1Ca resistant S. exigua. Among the clones from this last comparison, cDNA fragments belonging to a third APN (APN1 were detected. Using sequences obtained from the three APN cDNA fragments and degenerate primers for a fourth APN (APN3, the full length sequences of four S. exigua APN cDNAs were obtained. Northern blot analysis of expression of the four APNs showed complete absence of APN1 expression in the resistant insects, while the other three APNs showed similar expression levels in the resistant and susceptible insects. Conclusion We have cloned and characterized four different midgut APN cDNAs from S. exigua. Expression analysis revealed the lack of expression of one of these APNs in the larvae of a Cry1Ca-resistant colony. Combined with previous evidence that shows the importance of APN in the mode of action of B. thuringiensis toxins, these results suggest that the lack of APN1 expression plays a role in the resistance to Cry1Ca in this S. exigua colony.

  15. Globular adiponectin but not full-length adiponectin induces increased procoagulability in human endothelial cells.

    Science.gov (United States)

    Bobbert, Peter; Antoniak, Silvio; Schultheiss, Heinz-Peter; Rauch, Ursula

    2008-02-01

    Adiponectin (APN), a recently discovered adipocytokine, is present in human serum in a full length (fAPN) and a globular form (gAPN). gAPN is a proteolytic cleavage product of fAPN and seems to show independent biological activities compared to the properties of fAPN. The influence of gAPN and fAPN on procoagulability of cells is still unknown. This study examined the effect of gAPN and fAPN on the expression of tissue factor (TF), the initiator of the extrinsic coagulation system, in human umbilical vein endothelial cells (HUVECs). TF activity was measured by a chromogenic assay, TF mRNA by real-time PCR and TF protein by western blot. We found TF activity to be increased after activation by gAPN (3 microg/mL) compared to a non-stimulated control (169.0+/-19.23 U versus 501.9+/-38.95 U, p<0.001). Furthermore, TF mRNA and TF protein was increased dose-dependently after gAPN stimulation. The gAPN-induced rise of TF activity and TF mRNA was significantly reduced by inhibition of the MAP kinases ERK1/2, p38 and JNK. Contrary to gAPN, stimulation with fAPN did not lead to these procoagulant effects. In conclusion, gAPN increased TF transcription, expression and activity in HUVECs. Therefore, our data support the theory that gAPN but not fAPN supports the cellular procoagulability via TF upregulation.

  16. A oferta de educação para adultos em situação de privação de liberdade na Penitenciária Doutor Manoel Martins Lisboa Júnior no município de Muriaé em Minas Gerais

    OpenAIRE

    Mól, Márcio José Ladeira

    2013-01-01

    Esta dissertação de mestrado está inserida no estudo da oferta de educação escolar para adultos em situação de privação de liberdade. Considerando que no Brasil, não existe pena de prisão perpétua, o indivíduo sentenciado, depois de cumprimento de sua pena, retorna ao convívio social. Desta forma, ações como o trabalho e a educação se fazem necessárias na busca de sua reabilitação e geração de oportunidade para uma vida longe do crime. A legislação brasileira, seguindo acordos e tratados inte...

  17. The Application Effect of APN Consecutive Scheduling Level Management Mode in the Orthopedic Ward%APN连续排班层级管理模式在骨科病房中的应用效果

    Institute of Scientific and Technical Information of China (English)

    程梅娟

    2016-01-01

    Objective In the orthopedic ward of a continuous scheduling and hierarchical management mode of effect is analyzed.Methods 80 medical workers were randomly divided into two groups, one group according to the traditional model of nursing responsibility system scheduling, and set as control group, another group implements continuous scheduling and hierarchical management model for scheduling, and set as the experimental group, the nursing effect of two groups were analyzed. ResultsThe nursing for scheduling and hierarchical management mode, the experimental group in quality of care, family satisfaction, nursing disputes, etc, significantly better than the control group,P<0.05, which was with statistical signiifcance.Conclusion In the orthopedic ward of a continuous scheduling and hierarchical management mode, has ideal effect, is a good way to improve the quality of nursing work.%目的:针对在骨科病房当中实行连续排班以及层级管理的模式所具备的效果进行分析。方法随机选择我院当中的80名医护工作人员,分成两组,其中一组按照传统责任制的护理工作模式排班,并设为参照组,另一组则实行连续排班以及层级管理的模式进行排班,并设为实验组,将两组的护理效果进行对比分析。结果实行连续排班以及层级管理的护理模式后,实验组在护理质量、家属的满意程度、护理纠纷等方面优于参照组,P<0.05,具有统计学意义。结论在骨科病房当中实行连续排班以及层级管理的模式,具有理想效果,可提升护理工作的质量。

  18. Indicação cirúrgica otorrinolaringológica em um ambulatório para pacientes com síndrome da apnéia e hipopnéia obstrutiva do sono

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    Martinho Fernanda Louise

    2004-01-01

    Full Text Available OBJETIVO: Este trabalho tem como objetivo mostrar que o tratamento cirúrgico otorrinolaringológico é uma opção de tratamento para SAHOS e mostrar quais os procedimentos que podem ser realizados e suas indicações. FORMA DE ESTUDO: Clínico retrospectivo. MÉTODO: Realizamos um estudo retrospectivo em 223 pacientes atendidos no ambulatório de Otorrinolaringologia da UNIFESP, onde todos apresentavam polissonografia basal e foram submetidos a anamnese e exame físico otorrinolaringológico. A conduta terapêutica foi dividida em cirúrgica (procedimentos faríngeos, nasais e craniofaciais e não-cirúrgica (CPAP, aparelho intraoral e medidas gerais. RESULTADOS: Quase metade dos pacientes (100 pacientes - 44,8% recebeu indicação de algum procedimento cirúrgico, num total de 168 procedimentos, que foram indicados dependendo da gravidade da SAHOS. Desta forma, a uvulopalatofaringoplastia e a radiofreqüência de palato foram indicadas preferencialmente em pacientes com SAHOS leve e moderada e o avanço maxilomandibular em pacientes com SAHOS severa. A amigdalectomia e as cirurgias da cavidade nasal não variaram quanto à gravidade da SAHOS. CONCLUSÃO: O tratamento cirúrgico otorrinolaringológico é uma opção de tratamento para pacientes com SAHOS e pode ter intuito curativo ou coadjuvante a outras terapias, melhorando a adaptação ao CPAP. Os procedimentos cirúrgicos devem ser indicados de forma criteriosa baseando-se na gravidade da doença e nas alterações anatômicas da VAS e do esqueleto facial.

  19. Adiponectin Provides Cardiovascular Protection in Metabolic Syndrome

    OpenAIRE

    Yoshihisa Okamoto

    2011-01-01

    Adipose tissue plays a central role in the pathogenesis of metabolic syndrome. Adiponectin (APN) is a bioactive adipocytokine secreted from adipocytes. Low plasma APN levels (hypoadiponectinemia) are observed among obese individuals and in those with related disorders such as diabetes, hypertension, and dyslipidemia. APN ameliorates such disorders. Hypoadiponectinemia is also associated with major cardiovascular diseases including atherosclerosis and cardiac hypertrophy. Accumulating evidence...

  20. Spontaneous Perirenal Hemorrhage in Cauda Equina Syndrome: A Case Report

    OpenAIRE

    Seok, Hyun; Kim, Sang-hyun; Choi, Won Hyuck; Ko, Yong Jae

    2013-01-01

    Neurogenic bladder is a common cause of acute pyelonephritis (APN) in cauda equina syndrome (CES). Perirenal hemorrhage, a rare complication of APN, can be a life-threatening condition. To our knowledge, there is no previous report of perirenal hemorrhage as a complication of APN in CES. A 57-year-old male, diagnosed with CES, due to a L3 burst fracture 3 months earlier, was presented with fever and chills. His diagnosis was APN due to neurogenic bladder. After treatment for APN, he was trans...

  1. Diagnostic Performance of Gray-scale Sonographic Findings for the Detection of Acute Pyelonephritis

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hui Jin; Kim, Young Jun; Park, Hee Sun; Jeon, Hae Jeong; Park, Hyoung Keun; Paick, Sung Hyun; Kim, Hyeong Gon; Lho, Yong Soo; Jung, Sung Il [Konkuk University School of Medicine, Seoul (Korea, Republic of)

    2012-03-15

    To evaluate the diagnostic performance of gray-scale renal sonographic findings for the diagnosis of acute pyelonephritis (APN) by using computed tomography as a reference standard. We retrospectively reviewed gray-scale renal sonographic findings of 48 patients for the detection of APN. All patients had clinical symptoms such as fever, flank pain, or dysuria and were confirmed as APN by contrast- enhanced CT. The presence of sonographic findings such as renal swelling, alteration of the parenchymal echogenicity, wall thickening of the renal pelvis, loss of the renal sinus fat echogenicity, and loss of the corticomedullary differentiation were evaluated. We also categorized all patients into mild APN or severe APN groups according to the volume of the morbid renal parenchyma on contrast-enhanced CT, and evaluated the aforementioned sonographic findings between the two groups. Overall diagnostic sensitivity, specificity, and accuracy of gray-scale renal ultrasonography (US) for the detection of APN were 32.5%, 72.0%, and 58.5%, respectively. The sensitivity and specificity of each sonographic finding were measured for each group. Renal swelling sensitivity and specificity were 33.8% and 70.8% for the mild APN group, but 45.8% and 66.7% for the severe APN group. Sensitivity and specificity for alteration of the parenchymal echogenicity were 41.7% and 79.2% for the mild APN group, but 58.3% and 66.7% for the severe APN group. The sensitivity and specificity for wall thickening of the renal pelvis was 37.5% and 95.8% for the mild APN group, but 50.0% and 95.8% for the severe APN group. The sensitivity and specificity of loss of the renal sinus fat echogenicity were 12.5% and 83.3% for the mild APN group, but 12.5% and 91.7% for the severe APN group. The sensitivity and specificity of the loss of the corticomedullary differentiation were 12.5% and 95.8% for the mild APN group, but 20.8% and 75.0% for the severe APN group. There was no significant difference of gray

  2. Evolutionary diversification of aminopeptidase N in Lepidoptera by conserved clade-specific amino acid residues.

    Science.gov (United States)

    Hughes, Austin L

    2014-07-01

    Members of the aminopepidase N (APN) gene family of the insect order Lepidoptera (moths and butterflies) bind the naturally insecticidal Cry toxins produced by the bacterium Bacillus thuringiensis. Phylogenetic analysis of amino acid sequences of seven lepidopteran APN classes provided strong support for the hypothesis that lepidopteran APN2 class arose by gene duplication prior to the most recent common ancestor of Lepidoptera and Diptera. The Cry toxin-binding region (BR) of lepidopteran and dipteran APNs was subject to stronger purifying selection within APN classes than was the remainder of the molecule, reflecting conservation of catalytic site and adjoining residues within the BR. Of lepidopteran APN classes, APN2, APN6, and APN8 showed the strongest evidence of functional specialization, both in expression patterns and in the occurrence of conserved derived amino acid residues. The latter three APN classes also shared a convergently evolved conserved residue close to the catalytic site. APN8 showed a particularly strong tendency towards class-specific conserved residues, including one of the catalytic site residues in the BR and ten others in close vicinity to the catalytic site residues. The occurrence of class-specific sequences along with the conservation of enzymatic function is consistent with the hypothesis that the presence of Cry toxins in the environment has been a factor shaping the evolution of this multi-gene family.

  3. Adiponectin Provides Cardiovascular Protection in Metabolic Syndrome

    Directory of Open Access Journals (Sweden)

    Yoshihisa Okamoto

    2011-01-01

    Full Text Available Adipose tissue plays a central role in the pathogenesis of metabolic syndrome. Adiponectin (APN is a bioactive adipocytokine secreted from adipocytes. Low plasma APN levels (hypoadiponectinemia are observed among obese individuals and in those with related disorders such as diabetes, hypertension, and dyslipidemia. APN ameliorates such disorders. Hypoadiponectinemia is also associated with major cardiovascular diseases including atherosclerosis and cardiac hypertrophy. Accumulating evidence indicates that APN directly interacts with cardiovascular tissue and prevents cardiovascular pathology. Increasing plasma APN or enhancing APN signal transduction may be an ideal strategy to prevent and treat the cardiovascular diseases associated with metabolic syndrome. However, further studies are required to uncover the precise biological actions of APN.

  4. PENGARUH EKSTRAK JAMU TERHADAP AKTIVITAS SEL NATURAL KILLER DALAM MELISIS ALUR SEL LEUKIMIA (K-562 SECARA IN VITRO [The Effects of Commercial “Jamu” Extracts on Natural Killer Cell Activity in Lysing Leukemic Cell Line (K-562 in vitro

    Directory of Open Access Journals (Sweden)

    Elisa Veronica D.C. 2

    2002-04-01

    Full Text Available Natural killer (NK cell consitutes white blood cells which specifically functions in lysing tumor and virus invected cells. In this research, a commercial “Jamu” was tested to observe its effect on NK cells activity against leukemic cell lines (K562 in vitro. Jamu was extracted with hot water, diluted and added into cell cultures consisted of a mixture of human peripheric limphocyte cells, as the source of the effector NK cells, and K562 cell line i.e., the target cells which were cell line derived from human leukemia and had been labelled with H3-thymidine. The mixture of the cells were made by culturing the two cells at the ratio of 50:1 and 100 : 1, respectively. The results showed that lysing activity of NK cells in the presence of “Jamu” water extract measured as lysing percentage and lysing index increased only slightly, which were not statiscally significant. It should be considered that the test used in this research represents only a part of the lysing mechanism by NK cells against the target cells. An in vivo test for a period of time will be recessary to elucidate ffurther this NK cell activity.

  5. Gambaran Fungsi Jantung Kelinci Domestik Secara Ekhokardiogram pada Anestesi Propofol dan Isoflurane Jangka Panjang (ECHOCARDIOGRAM PROFILE OF CARDIAC FUNCTION IN DOMESTIC RABBITS DURING LONG-TERM ANESTHESIA PROPOFOL AND ISOFLURANE

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    Sitaria Siallagan

    2014-08-01

    Full Text Available Several major surgeries may require long time use of anesthetics and therefore increase the heartfunction suppression. The purpose of this study was to determine long term effects of anesthetic propofoland isoflurane on heart structure and function of domestic rabbits through observation of the blood flowvolume and contraction capabilities by M-mode echocardiography examination. This study used thecombination of propofol 12.5 mg/kg IV as an induction and isoflurane inhalation as the maintenanceanesthetic for a 12-hour observation. Parameters observed in this study were heart rate, wall thickness,internal chamber dimension, blood flow volume, fractional shortening, and ejection fraction. Results showedthat the left ventricle heart chamber dimensions during diastole (LVIDd and systole (LVIDs were between1.21±0.08 to 1.33±0.1 cm, and 0.87±0.08 to 0.98±0.08 cm respectively. Left ventricular wall thickness atdiastole (LVWd and systole (LVWs ranged from 0.24±0.04 to 0.28±0.04 cm, and 0.23±0.03 to 0.28±0.05cm respectively, while the thickness of the interventricular septa at diastole (IVSd and systole (IVSsranged from 0.23±0.02 to 0.27±0.04 cm, and 0.28±0.06 to 0.30±0.05 cm. Heart rate ranged from 244.6±18.46to 266.4±24.55 beat/min. Heart flow volumes which included stroke volume (SV and cardiac output (COranged from 1.07±0.29 to 1.57±0.53 mL/beat and 0.27±0.07 to 0.39±0.14 L/min respectively. Fractionalshortening and ejection fraction ranged from 26±3 to 31±5% and 60±2 to 67±8% respectively. In conclusion,long term anesthesic of propofol and isoflurane combination induced minimum cardiac suppression indomestic rabbits.

  6. Gambaran Patologi Bursa Fabricius Embrio Ayam Pascavaksinasi Gumboro Secara In Ovo Menggunakan Vaksin Lokal dan Komersial (PATHOLOGIC DESCRIPTION OF BURSA FABRICIUS CHICKEN EMBYROS AFTER IN OVO VACCINATED WITH LOCAL AND COMMERSIAL GUMBORO VACCINES

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    Sutiastuti Wahyuwardani

    2015-10-01

    Full Text Available Bursa Fabricius is a target organ of gumboro virus infection which is often damaged after vaccinationusing hot intermediate gumboro live vaccine. The purpose of this study was to assess pathologic effect oflocal and commercial gumboro vaccines . As many as 45 embryo chicken eggs at nine day old were used inthis research, then grouped into three groups of 15 embryos chicken eggs each, these were: Embryo chickeneggs without vaccination (Group I, vaccinated with IBD intermediate plus commercial vaccine (Group IIand IBD intermediate plus local vaccine (Group III. Vaccinations were done at 14 days old. All groups thenterminated each three embryos at 12 hours, 1, 2, 3 days post vaccination. The results showed that pathologicanatomic lesions could not be detected. Whereas pathologic lesions were detected in the group that werevaccinated with intermediate plus local IBD observed more severe than in the group that vaccinated withintermediate plus commercial IBD. Lesions such as edema, hemorrhages, necrosis of lymphoid cells wereobserved microscopically in embryo at 12 hours, 1, 2 and 3 days post vaccination in Group II and group III.The lesions were more severe at two days post vaccination causing some lymphoid follicles disappeared at three days post vaccination. However, they were not detected again in the bursa Fabricius three days afterhatching. Cells containing antigens of gumboro were detected in the bursa Fabricius of chicken embryo atone day until three days post vaccination, then disappeared after three days post hatch. It was concludedthat pathologic description of bursa fabricius showed that virus vaccines used for vaccinated IBD in ovowere still virulent, that can cause histopathologic lesions. The viruses are suggested to be more attenuatedbefore using as vaccine in ovo.

  7. Induksi Ekstrak Pegagan Secara in vitro terhadap Proliferasi dan Diferensiasi Sel-Sel Otak Besar Anak Tikus (IN VITRO INDUCTION OF CENTELLA ASIATICA (PEGAGAN EXTRACT ON THE PROLIFERATION AND DIFFERENTIATION OF NEWBORN RAT CORTEX CEREBRI CELLS

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    Ita Djuwita

    2013-09-01

    Full Text Available The aim of the study was to analyze the potency of Centella asiatica extract to induce proliferation andneurogenesis process of newborn rats cortex cerebri.   Research has been conducted on in vitro culture ofthree days old rat (Sprague Dawley cerebrum cells in DMEM (Dulbecco’s Modiûed Eagle’s Mediumcontaining 10% NEAA (Non Essential Amino Acid, 1 mM NaHCO3, 10% NBCS (Newborn Calf Serumand 50 µg/mL gentamycin (mDMEM, with and without Centella asiatica (CA leaf extracts. The experimentwas set in five groups of treatment consisted of positive control (mDMEM+30 µg/mL asiaticoside (AC,negative control (mDMEM, and mDMEM with three concentration of CA extract i.e. 100 ppm, 200 ppmand 400 ppm. Culture was done in 5% CO2 incubator at 37oC for six days. The parameters observed werecells proliferation based on Population Doubling Time (PDT, neuron and glia composition, and the lengthof axon and dendrite. Cells concentration were counted using Newbauer hemocytometer.  Neuron and gliacells were determined based on morphology after Hematoxylin-Eosin staining, and the length of axon anddendrite were measured using eyepiece micrometer. Data were analyzed using ANOVA and Duncan test.The results showed that Centella asiatica extract at concentration 100 ppm could induce neurogenesisand increased the axon length growth.  However, at concentration 200 and 400 ppm, CA extract  inhibitedthe neuronal cells proliferation and the axonal growth (P<0,05. In conclusion, induction of Centella asiaticaextracts at concentration of 100 ppm on the cortex cell cerebrum cells culture increase the axon lengthgrowth and tends to induce neurogenesis; however at higher concentration CA extract was neurotoxic.

  8. Lokasi Gigitan Secara Anatomi dan Waktu Kematian Pascagigitan Anjing Rabies pada Korban Manusia di Bali (THE ANATOMICAL LOCATIONS OF BITE AND THE TIME OF DEATH IN HUMAN VICTIMS BITTEN BY RABIES INFECTED DOGS IN BALI

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    I Ketut Suatha

    2015-05-01

    Full Text Available Rabies was a new emerging disease in Bali. After the first case of rabies in 2008 there were morethan one hundred of human victims in Bali. The aim of this study was to report the anatomical locationof human body bitten by rabies infected dogs in Bali, and also the day of victims death after the bitten. Aretrospective cross-sectional review of rabies incidences from September 2008 to the end of 2011 was usedin this study. A total of 122 rabies human victims data were used as a population sample. The data on theprofiles of individuals victim were colected , such as the age, gender, living place, anatomical bite site onhuman body, bite date, and type of biting animal. The obtained data were analyzed using descriptiveanalysis. The result showed that the anatomical location of human body that bitten by rabies infected dogwas occurred on leg (52%, hand (32%, body (6%, and head (4%. Of 122 victims, 61.5% were men and38.5% were woman. The death time of the human rabies victims in average occurred at day 95th after thebite date, and time of the victims death depended on the anatomical location of the bite. Bite that occurredon the head caused death in average on day 19th, on the body on day 83th, on the hand on day 122nd, and onleg on day 166th. In conclusion, the most victims of human rabies in Bali was men and the death was foundoccurred on day 95th after the bite date. The bite at the head caused death more quickly than the other partof human body.

  9. Kajian Isolat Bakteri Asam Laktat dalam Menurunkan Kolesterol Secara In Vitro dengan Keberadaan Oligosakarida (A Study In Vitro of Lactic Acid Bacteria (LAB) Isolates on Cholesterol Lowering Ability in The Presence of Oligosaccharides)

    OpenAIRE

    Yati Maryati; Lilis Nuraida1,2)*; Ratih Dewanti Hariyadi

    2016-01-01

    This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the pe...

  10. Pengaruh Penambahan Chorionic Gonadotrophin pada Medium Maturasi terhadap Kemampuan Maturasi, Fertilisasi, dan Perkembangan Embrio secara In Vitro Kambing Peranakan Ettawa (The Effect of Chorionic Gonadotrophin Addition Into Maturation Medium on The Abili

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    Nurvina Septi Adifa

    2012-02-01

    the values of 40.8%, 11.4%, and 12.2% respectively. Based on the result it could be concluded that chorionic gonadotrophin addition into maturation medium had not increased ettawa crossbred oocytes maturation, fertilization, and in vitro cleavage rate. The best maturation, fertilization, and in vitro cleavage rate were found using maturation medium without any addition of chorionic gonadotrophin. (Key words: Does oocyte, Chorionic gonadotrophin, In vitro maturation, In vitro fertilization, In vitro embryo development

  11. Pemanfaatan Biogas (Gas Methan) Dari Hasil Pengolahan Palm Oil Mill Effluent (Pome) Secara Anaerobic Sebagai Bahan Bakar Unit Oil Refinery Dan Pencegah Pencemaran Lingkungan Di Pt.Multimas Nabati Asahan, Batu Bara

    OpenAIRE

    sitorus, Syaiful bahri

    2016-01-01

    Palm oil production in Indonesia continues to increase, thereby increasing the amount of waste therefore to decimate environmental pollution load in the waste in the processing of palm oil in the palm oil mills in PT.Multimas Nabati Asahan, Batu Bara has made Palm Oil Mill Effluent (POME) by using Anaerobic technology to produce biogas which can be utilized as fuel for burning palm oil mills in the refinery unit Multimas 200 palm oil mills, once the reduced pollutant load will be processed b...

  12. Pengaruh Penambahan Minyak Kelapa, Minyak Biji Bunga Matahari, dan Minyak Kelapa Sawit terhadap Penurunan Produksi Metan di dalam Rumen secara in Vitro (The Effect of Addition Coconut Oil, Sunflower Seed Oil, and Palm Olein on Reducing Ruminal Methane Pro

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    Puput Diah Sitoresmi

    2012-02-01

    protozoa growth and addition oil up to 5.0% reduced methane production as much as 15.80%. (Key words : Coconut oil, Sunflower seed oil, Palm olein, Methane production, Protozoa count, In vitro fermentation

  13. PENGARUH PEMULIHAN GIZI BURUK RAWAT JALAN SECARA KOMPREHENSIF TERHADAP KENAIKAN BERAT BADAN, PANJANG BADAN, DAN STATUS GIZI ANAK BATITA (EFECTS OF COMPREHENSIVE OUTPATIENT CARE ON WEIGHT AND HEIGHT INCREAMENT, AND NUTRITIONAL STATUS AMONG SEVERELY MALNO

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    Arnelia Arnelia

    2013-07-01

    Full Text Available ABSTRACT Background: Outpatient care is a new approach for severely acute malnourished children while the other is inpatient care. To increase and optimalize outpatient care at Nutrition Clinic in Center for R&D in Nutrition and Food (CRDNF Bogor, the comprehensively treatment was performed including health, nutrition and caring practices. Objectives: To analyse weight and height increament and the nutritional status of under-three years children during outpatient care of severely malnourished children. Methods: An intervention study was implemented to severely malnourished children who participating in a 6 months outpatient care at Nutrition Clinic in CRDNF, Bogor. The comprehensive treatments were: curing the illness, nutrition and health counseling, gradually dietary treatment, caring guidance. The control groups were treated as the regular treatment of the clinic, including curing the illness, nutrition and health counseling and skim milk ration. Results: The average weight increament among comprehensive group significantly higher than those of reguler group, that was 1.39 ± 0.66 kg and 0.80 ± 0.40 after 3 months  (p=0.001 and at the end of out patient care was increased 2.02 ± 0.85 kg and 1.39 ± 0.52 consecutively (p=0.008. No different was found on the increament of childs length/height after 6 months out patient care, that was 4.0 ± 2.0 cm dan 4.1 ± 1.3 cm (p=0.806. After 3 months, 58.3% of comprehensive group and 22.7% of reguler group increased their weight by >15%, and to 73.9% and 50% after 4 months intervention. Based on W/H category, 79.2% of the comprehensive groups were severe wasting while in reguler group 59.1% and the rest were wasting at the beginning of the study. After 3 months intervention, 50% of the comprehensive group and 27.3% of the reguler group were normal and by the end increased to 73.9% and 33.3%. Conclusion: The increament of weight and the nutritional status improvement was much better among comprehensive care than that of the regular care. [Penel Gizi Makan 2010, 33(2: 125-137]   Keywords: severe acute malnutrition, severe wasting, out-patient, comprehensive

  14. KOMPOSISI KIMIA DAN KRISTALINITAS TEPUNG PISANG TERMODIFIKASI SECARA FERMENTASI SPONTAN DAN SIKLUS PEMANASAN BERTEKANAN-PENDINGINAN (Chemical Composition and Crystallinity of Modified Banana Flour by Spontaneous Fermentation and Autoclaving-Cooling Cycle

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    Nurhayati Nurhayati

    2014-07-01

    (39,13 – 42,68% bk dibandingkan dengan yang satu siklus (29,34 – 35,93% bk. Proses pemanasan bertekananpendinginan menurunkan kristalinitas tepung pisang dari 18,74-20,08% menjadi 6,98-9,52%. Difraksi sinar X menunjukkan granula pati pisang adalah granula tipe C yang merupakan campuran dari granula tipe A dan tipe B. Kata kunci: Tepung pisang termodifikasi, fermentasi spontan, autoclaving-cooling cycles

  15. Backwash Effects of Portfolio Assessment in Academic Writing Classes

    Science.gov (United States)

    Syafei, Muh

    2012-01-01

    This paper discusses a study investigating students' opinions and reflections on backwash effects of portfolio assessments applied in Academic Writing course. To obtain the data, the researcher carried out interviews with 70 students of English Education Department (EED) of "Universitas Muria Kudus" (UMK) who took Academic Writing I and…

  16. Backwash Effects of Portfolio Assessment in Academic Writing Classes

    Science.gov (United States)

    Syafei, Muh

    2012-01-01

    This paper discusses a study investigating students' opinions and reflections on backwash effects of portfolio assessments applied in Academic Writing course. To obtain the data, the researcher carried out interviews with 70 students of English Education Department (EED) of "Universitas Muria Kudus" (UMK) who took Academic Writing I and…

  17. Application of the phylogenetic species concept to Wallemia sebi from house dust and indoor air revealed by multi-locus genealogical concordance

    NARCIS (Netherlands)

    Nguyen, Hai D T; Jančič, Sašo; Meijer, Martin; Tanney, Joey B; Zalar, Polona; Gunde-Cimerman, Nina; Seifert, Keith A

    2015-01-01

    A worldwide survey of Wallemia occurring in house dust and indoor air was conducted. The isolated strains were identified as W. sebi and W. muriae. Previous studies suggested that the W. sebi phylogenetic clade contained cryptic species but conclusive evidence was lacking because only the internal t

  18. High molecular weight adiponectin correlates positively with myeloperoxidase in patients with type 2 diabetes mellitus.

    Science.gov (United States)

    Bobbert, P; Rauch, U; Stratmann, B; Goldin-Lang, P; Antoniak, S; Bobbert, T; Schultheiss, H P; Tschoepe, D

    2008-11-01

    Adiponectin (APN) is present in human plasma as a low molecular weight (LMW), a middle molecular weight (MMW) and a high molecular weight form (HMW). As a support to determine properties such as anti-atherogenic or atherogenic effects, recent clinical studies suppose to determine the ratio of each APN multimer to total APN but not the absolute plasma concentration of APN. In the present study, the correlation of APN and its multimers with myeloperoxidase (MPO), an enzyme with pro-inflammatory properties, was examined in patients with type 2 diabetes mellitus. MPO and APN serum levels were assessed in 49 patients with type 2 diabetes mellitus at the beginning and at the end of an anti-diabetic treatment. After treatment a significant increase in the ratio of HMW to total APN (from 0.43+/-0.16 to 0.59+/-0.14, p<0.05) was found. Before treatment, HMW-APN was correlated positively with MPO (r=0.314, p<0.05). Moreover, a positive correlation was observed between the increased HMW ratio and MPO during treatment (r=0.304, p<0.05). HMW-APN correlates positively with MPO in patients with type 2 diabetes. Therefore, HMW-APN may exert possible pro-inflammatory effects in type 2 diabetes.

  19. Factors influencing advanced practice nurses' ability to promote evidence-based practice among frontline nurses.

    Science.gov (United States)

    Gerrish, Kate; Nolan, Mike; McDonnell, Ann; Tod, Angela; Kirshbaum, Marilyn; Guillaume, Louise

    2012-02-01

    Advanced practice nurses (APNs) have an important role in promoting evidence-based practice (EBP) among frontline nurses (FLNs). Factors influencing FLNs' engagement with EBP are well documented but little is known about factors that affect APNs' ability to facilitate evidence in practice. To identify factors that influence APNs' ability to promote EBP among FLNs. A multiple case study of 23 APNs from hospital and primary care settings across seven English health authorities was undertaken. Data collection comprised interviews and observation of APNs and interviews with FLNs and other healthcare professionals. Data were analysed using the Framework approach. Four groups of influencing factors were identified: (1) Personal attributes of APNs included knowledge and skills in EBP, clinical credibility with frontline staff and leadership style. (2) Relationships with stakeholders included APNs' interactions with FLNs and the level of support from managers and medical colleagues. (3) Aspects of the APN role included their sphere of responsibility and workload. (4) Organisational context included the organisational culture, FLNs' workload, professional networks and available resources. Educational preparation for APNs should enable them to develop expertise in EBP plus interpersonal and leadership skills to manage relational dynamics in clinical settings. APN role specifications should provide the opportunity to promote EBP. The organisational culture should be conducive to enabling EBP with managers supportive of this aspect of the APNs' role. APNs need to be supported to address the individual, interpersonal and organisational factors, which influence their ability to promote EBP. Organisational commitment at the highest level is key to APNs' ability to fulfil this aspect of their role. ©2011 Sigma Theta Tau International.

  20. ANALISIS PERMINTAAN PERJALANAN PENGGUNA JASA KERETA API EKSEKUTIF RUTE SEMARANG-JAKARTA

    Directory of Open Access Journals (Sweden)

    MG. Endang Sri Utari

    2017-06-01

    Full Text Available Penelitian ini berjutuan menganalisis pengaruh pendapatan konsumen, harga tiket kereta api, persepsi harga tiket pesawat, kualitas layanan, jenis kereta api, dan adanya tiket promo pesawat terhadap permintaan kereta api eksekutif Argo Muria dan Argo Sindoro Rute Semarang-Jakarta. Populasi penelitian ini adalah rata-rata penumpang Argo Muria dan Argo Sindoro per har, sedangkan jumlah sampel 160 orang diambil menggunakan rumus slovin dan teknik simple random sampling. Metode pengumpulan data menggunakan angket, wawancara dan dokumentasi. Metode analisis menggunakan analisis regresi Ordinary Least Square (OLS. Hasil penelitian menunjukkan bahwa pendapatan konsumen, harga tiket kereta api Argo Muria dan Argo Sindoro serta adanya tiket promo pesawat berpengaruh terhadap jumlah permintaan kereta api eksekutif Argo Muria dan Argo Sindoro. Using Ordinary Least Square (OLS this paper aims to analyze the effect of: railway passenger’s income, railway fares, perception of air travel fares, railway service performance, types of trains and air promo travel tickets to the demand of executive class railway route Semarang-Jakarta. The population of this research are passenger of Argo Muria and Argo Sindoro an average each day, amount of sample are 160 people was taken by slovin formula and simple random sampling. The method of accumulating data used questionnaire, interview and documentation. The result shows that the railway passenger’s income have positive income to executive railway demand. An increase executive railway fares will result in decrease executive railway demand. The air promo travel tickets have positive effect to difference of executive class railway demand caused passenger’s decision to move in air plane or unturned by railway.

  1. USSR Report, International Economic Relations, No. 61

    Science.gov (United States)

    2007-11-02

    INTERNATIONAL ECONOMIC RELATIONS No. 61 CONTENTS USSR- CEMA TRADE CEMA Progress in 1982 Spotlighted (APN DAILY REVIEW, 8 Aug 83) 1 Plant...Director Views Relations With CEMA Counterparts (V. P. Kabaidze Interview; PRAVDA, 27 Jun 83) 3 Soviet, CEMA Cooperation With Cuba (V. Morozov; APN...DAILY REVIEW, 29 Jul 83) 8 CEMA Specialization, Integration Noted (N. Syomin; APN DAILY REVIEW, 12 May 83) 12 TRADE WITH IDC’S

  2. Primary care physician assistant and advance practice nurses roles: Patient healthcare utilization, unmet need, and satisfaction.

    Science.gov (United States)

    Everett, Christine M; Morgan, Perri; Jackson, George L

    2016-12-01

    Team-based care involving physician assistants (PAs) and advance practice nurses (APNs) is one strategy for improving access and quality of care. PA/APNs perform a variety of roles on primary care teams. However, limited research describes the relationship between PA/APN role and patient outcomes. We examined multiple outcomes associated with primary care PA/APN roles. In this cross-sectional survey analysis, we studied adult respondents to the 2010 Health Tracking Household Survey. Outcomes included primary care and emergency department visits, hospitalizations, unmet need, and satisfaction. PA/APN role was categorized as physician only (no PA/APN visits; reference), usual provider (PA/APN provide majority of primary care visits) or supplemental provider (physician as usual provider, PA/APN provide a subset of visits). Multivariable logistic and multinomial logistic regressions were performed. Compared to people with physician only care, patients with PA/APNs as usual providers [5-9 visits RRR=2.4 (CI 1.8-3.4), 10+ visits RRR=3.0 (CI 2.0-4.5): reference 2-4 visits] and supplemental providers had increased risk of having 5 or more primary care visits [5-9 visits RRR=1.3 (CI 1.0-1.6)]. Patients reporting PA/APN as supplemental providers had increased risk of emergency department utilization [2+ visits: RRR 1.8 (CI 1.3, 2.5)], and lower satisfaction [very dissatisfied: RRR 1.8 (CI 1.03-3.0)]. No differences were seen for hospitalizations or unmet need. Healthcare utilization patterns and satisfaction varied between adults with PA/APN in different roles, but reported unmet need did not. These findings suggest a wide range of outcomes should be considered when identifying the best PA/APN role on primary care teams. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Adiponectin promotes coxsackievirus B3 myocarditis by suppression of acute anti-viral immune responses.

    Science.gov (United States)

    Jenke, A; Holzhauser, L; Löbel, M; Savvatis, K; Wilk, S; Weithäuser, A; Pinkert, S; Tschöpe, C; Klingel, K; Poller, W; Scheibenbogen, C; Schultheiss, H P; Skurk, C

    2014-05-01

    Adiponectin (APN) is an immunomodulatory adipocytokine that improves outcome in patients with virus-negative inflammatory cardiomyopathy and mice with autoimmune myocarditis. Here, we investigated whether APN modulates cardiac inflammation and injury in coxsackievirus B3 (CVB3) myocarditis. Myocarditis was induced by CVB3 infection of APN-KO and WT mice. APN reconstitution was performed by adenoviral gene transfer. Expression analyses were performed by qRT-PCR and immunoblot. Cardiac histology was analyzed by H&E-stain and immunohistochemistry. APN-KO mice exhibited diminished subacute myocarditis with reduced viral load, attenuated inflammatory infiltrates determined by NKp46, F4/80 and CD3/CD4/CD8 expression and reduced IFNβ, IFNγ, TNFα, IL-1β and IL-12 levels. Moreover, myocardial injury assessed by necrotic lesions and troponin I release was attenuated resulting in preserved left ventricular function. Those changes were reversed by APN reconstitution. APN had no influence on adhesion, uptake or replication of CVB3 in cardiac myocytes. In acute CVB3 myocarditis, cardiac viral load did not differ between APN-KO and WT mice. However, APN-KO mice displayed an enhanced acute immune response, i.e. increased expression of myocardial CD14, IFNβ, IFNγ, IL-12, and TNFα resulting in increased cardiac infiltration with pro-inflammatory M1 macrophages and activated NK cells. Up-regulation of cardiac CD14 expression, type I and II IFNs and inflammatory cell accumulation in APN-KO mice was inhibited by APN reconstitution. Our observations indicate that APN promotes CVB3 myocarditis by suppression of toll-like receptor-dependent innate immune responses, polarization of anti-inflammatory M2 macrophages and reduction of number and activation of NK cells resulting in attenuated acute anti-viral immune responses.

  4. The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.

    Science.gov (United States)

    Zou, Wei; Xiao, Zuoqi; Wen, Xiaoke; Luo, Jieying; Chen, Shuqiong; Cheng, Zeneng; Xiang, Daxiong; Hu, Jian; He, Jingyu

    2016-11-25

    Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism. Thirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted. APN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway. APN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.

  5. E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Hiroyasu; Kuroda, Nana; Uekita, Hiromi; Kochi, Ikoi; Matsumoto, Akane; Niinaga, Ryu [Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka (Japan); Funahashi, Tohru; Shimomura, Iichiro [Department of Metabolic Medicine, Osaka University Graduate School of Medicine, Osaka (Japan); Kihara, Shinji, E-mail: skihara@sahs.med.osaka-u.ac.jp [Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka (Japan)

    2016-02-05

    Background: Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although anti-diabetic effects are mostly mediated by the adiponectin receptors AdipoR1 and AdipoR2, the anti-atherogenic mechanisms have not been fully elucidated. Methods and Results: In this study, we identified E-selectin ligand (ESL)-1 as a novel APN-binding protein by mass spectrometry analysis of HepG2 cell-derived immunoprecipitant with an anti-APN antibody. Cell adhesion assays using fluorescence-labelled monocyte cell line THP-1 cells and human umbilical vein endothelial cells (HUVECs) revealed that APN-pre-treated THP-1 cells had reduced binding ability to HUVECs. This APN-mediated suppressive effect on monocyte binding to endothelial cells was partially abrogated by targeting ESL-1 with shRNA in THP-1 cells. In addition, serial mutagenesis analysis disclosed that five extracellular amino acids close to the N-terminus of ESL-1 were essential for binding with APN. Conclusion: Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN. - Highlights: • E-selectin ligand (ESL)-1 was identified as an adiponectin (APN)-binding protein. • ESL-1 bound to APN at its N-terminal 6th-10th amino acids. • shESL-1 reduced the suppressive effect of APN on adhesion of THP-1 cells to HUVECs. • Interaction with ESL may be involved in the anti-atherogenic effects of APN.

  6. Perivascular adipose tissue-derived adiponectin inhibits collar-induced carotid atherosclerosis by promoting macrophage autophagy.

    Directory of Open Access Journals (Sweden)

    Changlong Li

    Full Text Available Adiponectin (APN secreted from perivascular adipose tissue (PVAT is one of the important anti-inflammatory adipokines to inhibit the development of atherosclerosis, but the underlying mechanism has not been clarified. In this study, we aimed to elucidate how APN regulates plaque formation in atherosclerosis.To assess the role of APN secreted by PVAT in atherosclerosis progression, we performed PVAT transplantation experiments on carotid artery atherosclerosis model: ApoE knockout (ApoE-/- mice with a perivascular collar placement around the left carotid artery in combination with a high-fat diet feeding. Our results show that the ApoE-/- mice with PVAT derived from APN knockout (APN-/- mice exhibited accelerated plaque volume formation compared to ApoE-/- mice transplanted with wild-type littermate tissue. Conversely, autophagy in macrophages was significantly attenuated in ApoE-/- mice transplanted with APN-/- mouse-derived PVAT compared to controls. Furthermore, in vitro studies indicate that APN treatment increased autophagy in primary macrophages, as evidenced by increased LC3-I processing and Beclin1 expression, which was accompanied by down-regulation of p62. Moreover, our results demonstrate that APN promotes macrophage autophagy via suppressing the Akt/FOXO3a signaling pathway.Our results indicate that PVAT-secreted APN suppresses plaque formation by inducing macrophage autophagy.

  7. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity

    Directory of Open Access Journals (Sweden)

    Kang Tang

    2017-01-01

    Full Text Available Haemorrhagic fever with renal syndrome (HFRS is characterised by an uncontrolled immune response that causes vascular leakage. Adiponectin (APN is an adipocytokine involved in prorevascularisation and immunomodulation. To investigate the possible effects of APN in the pathogenesis of HFRS, total and high molecular weight (HMW APN levels in the plasma of patients with HFRS were quantified using enzyme-linked immunosorbent assay (ELISA. Compared with those in healthy controls, the plasma total and HMW APN levels in patients were elevated to different degrees from the fever onset and remained high at the convalescent phase. Consistent with these results, western blot analysis additionally showed that low molecular weight (LMW, middle molecular weight (MMW, and HMW APN levels were all elevated and contributed to the elevation of the total APN level. Importantly, sustained high levels of total and HMW APN at the convalescent phase were significantly higher in patients with critical disease than those in patients with mild or moderate disease. Moreover, total and HMW APN levels negatively correlated with white blood cell count and positively correlated with platelet count and serum albumin level. These results may provide insights into understanding the roles of total and HMW APN in the pathogenesis of HFRS.

  8. Diffusion-weighted imaging and apparent diffusion coefficient values versus contrast-enhanced MR imaging in the identification and characterisation of acute pyelonephritis

    Energy Technology Data Exchange (ETDEWEB)

    Faletti, Riccardo; Cassinis, Maria Carla; Fonio, Paolo; Grasso, Angela; Battisti, Giacomo; Bergamasco, Laura; Gandini, Giovanni [University of Torino, Radiology Institute, Department of Surgical Sciences, Torino (Italy)

    2013-12-15

    To compare contrast-enhanced (CEMR) and diffusion-weighted (DWI) magnetic resonance imaging in diagnosing acute pyelonephritis (APN) and to assess ADC measurement reliability in differentiating among normal renal parenchyma, APN and abscesses. Eighty-eight patients (80 women, mean age 36.5 years) with clinical suspicion of APN were retrospectively reviewed. An experienced observer quantified lesions with a score ranging from 0 to 3. DWI and CEMR were compared on the basis of the assigned score, total examination number (88) and on presence (36)/absence (52) of abscesses. ADC values, calculated at the healthy parenchyma, APN and abscessed were compared to each other. Agreement between CEMR and DWI was 94.3 % (83/88 patients; P < 0.05). In the APN group, DWI was awarded the highest visibility score compared to CEMR (P = 0.05), while in the abscess group CEMR had the highest score (P = 0.04). The difference between ADC values of the APN-healthy parenchyma and abscess-APN groups was significant (P < 0.05). The area under the ROC curve of ADC values of the APN-healthy and abscess-APN groups were found to be 0.94 (95 % CI; cutoff value = 2) and 0.78 (95 % CI; cutoff value = 1.2) respectively. Diffusion-weighted imaging appears reliable in the diagnosis and follow-up of acute pyelonephritis and could provide a reasonable alternative to contrast-enhanced magnetic resonance imaging. (orig.)

  9. 不同严重度阻塞性睡眠呼吸暂停低通气综合征中年患者的睡眠结构分析%Sleep stages studies to middle age individual with different severity of Obstructiv sleep apne-a/hypopnea syndro me

    Institute of Scientific and Technical Information of China (English)

    黄敏方; 黄懂生; 付丽丽

    2015-01-01

    Objective To detect percentage of sleep stages between different severity of middle age Obstructive Sleep Apnea/Hypopnea Syndrome(OSAHS)and compare percentage of sleep stages in OSAHS with snore and middle age normal sleeping individual.Methods 58 OSAHS patients who were 40~49 years old,would be treated with mandibular advancing oral appliance in department of Orthodontics,Peoples Hospital of Guangxi Zhuang Autonomous Region from 2004-2014 year were se-lected.They included two group:mild to middle severity(≤ 5 events/hour,respiratory apnea/hypopnea index(AHI)<30 events/hour)34 cases,heavy severity [≤ 30 events/hour,respiratory apnea/hypopnea index(AHI)]24cases.Time percentage of sleep stages S1、S2、S3-4、REM(rapid eye movement stage)in whole night sleeping were compared between two group and normal sleeping group with same OSAHS age range in SPSS 13.0.Independent sample T test were doned.Differences of sleep stages be-tween four group were found.Results There arent any significant difference in time percentage of Stages S1、S2、S3-4、REM between two OSAHS groups.S1 time percentage of mild to middle group in the whole night sleeping is lower,S2 is higher and REM of heavy group is lower than normal group.S2 time percentage of heavy group is lower than mild-middle group.Conclusion Rapid-wane and REM sleep stage in OSAHS are shorter comparatively than normal sleep group.The more severity of OSAHS,the more shorter of S2 sleep stage and longer of S1 sleep stage.%目的:探讨不同严重程度的阻塞性睡眠呼吸暂停低通气综合征(OSAHS)中年患者的睡眠结构特点,并与正常人的睡眠结构作比较。方法选取在2004年至2014年间来广西壮族自治区人民医院口腔正畸科拟行口腔矫治器治疗的40~49岁 OSAHS 患者58例,其中轻中度34例,重度24例,分别对这2组患者及睡眠正常人的睡眠结构 S1、S2、S3和快速动眼(REM)分期的百分比作比较,探讨他们的睡眠结构的差异。结果与正常同龄人相比,轻中度 OS-AHS 患者浅度睡眠占睡眠比例较低,中度睡眠较高;重度 OSAHS 患者浅度、快动眼期睡眠百分比较低。重度 OSAHS 患者的中度睡眠(S2期)百分比较轻中度 OSAHS 患者为低。结论OSAHS 患者的快波睡眠及动眼期睡眠与正常同龄人相比相对缩短。随病情加重,中度睡眠相对减少,浅度睡眠增加。

  10. Curriculum vitae: An important tool for the nurse practitioner.

    Science.gov (United States)

    Hicks, Rodney W; Roberts, Mary Ellen E

    2016-07-01

    Advanced practice nurses (APNs) should maintain a curriculum vitae (CV) that comprehensively reflects the individual's work and professional accomplishments. This article guides APNs through best practices for development of a CV. Tips are offered to help guide the content, format, and maintenance of the CV.

  11. Impaired adiponectin signaling contributes to disturbed catabolism of branched-chain amino acids in diabetic mice.

    Science.gov (United States)

    Lian, Kun; Du, Chaosheng; Liu, Yi; Zhu, Di; Yan, Wenjun; Zhang, Haifeng; Hong, Zhibo; Liu, Peilin; Zhang, Lijian; Pei, Haifeng; Zhang, Jinglong; Gao, Chao; Xin, Chao; Cheng, Hexiang; Xiong, Lize; Tao, Ling

    2015-01-01

    The branched-chain amino acids (BCAA) accumulated in type 2 diabetes are independent contributors to insulin resistance. The activity of branched-chain α-keto acid dehydrogenase (BCKD) complex, rate-limiting enzyme in BCAA catabolism, is reduced in diabetic states, which contributes to elevated BCAA concentrations. However, the mechanisms underlying decreased BCKD activity remain poorly understood. Here, we demonstrate that mitochondrial phosphatase 2C (PP2Cm), a newly identified BCKD phosphatase that increases BCKD activity, was significantly downregulated in ob/ob and type 2 diabetic mice. Interestingly, in adiponectin (APN) knockout (APN(-/-)) mice fed with a high-fat diet (HD), PP2Cm expression and BCKD activity were significantly decreased, whereas BCKD kinase (BDK), which inhibits BCKD activity, was markedly increased. Concurrently, plasma BCAA and branched-chain α-keto acids (BCKA) were significantly elevated. APN treatment markedly reverted PP2Cm, BDK, BCKD activity, and BCAA and BCKA levels in HD-fed APN(-/-) and diabetic animals. Additionally, increased BCKD activity caused by APN administration was partially but significantly inhibited in PP2Cm knockout mice. Finally, APN-mediated upregulation of PP2Cm expression and BCKD activity were abolished when AMPK was inhibited. Collectively, we have provided the first direct evidence that APN is a novel regulator of PP2Cm and systematic BCAA levels, suggesting that targeting APN may be a pharmacological approach to ameliorating BCAA catabolism in the diabetic state.

  12. A Qualitative Analysis of an Advanced Practice Nurse-Directed Transitional Care Model Intervention

    Science.gov (United States)

    Bradway, Christine; Trotta, Rebecca; Bixby, M. Brian; McPartland, Ellen; Wollman, M. Catherine; Kapustka, Heidi; McCauley, Kathleen; Naylor, Mary D.

    2012-01-01

    Purpose: The purpose of this study was to describe barriers and facilitators to implementing a transitional care intervention for cognitively impaired older adults and their caregivers lead by advanced practice nurses (APNs). Design and Methods: APNs implemented an evidence-based protocol to optimize transitions from hospital to home. An…

  13. Inverse Levels of Adiponectin in Type 1 and Type 2 Diabetes Are in Accordance with the State of Albuminuria

    Science.gov (United States)

    Jazbec, Anamarija; Tomic, Martina; Piljac, Ante; Jurisic Erzen, Dubravka; Novak, Branko; Kastelan, Snjezana; Lovrencic, Marijana Vucic; Brkljacic, Neva

    2015-01-01

    Aims. To investigate the behaviour of adiponectin (ApN) in patients with type 1 and type 2 diabetic nephropathy. Methods. ApN and inflammatory and other markers of the metabolic syndrome were compared across diabetes types, albumin excretion rate (AER), and creatinine clearance (CrCl) categories in 219 type 1 and type 2 diabetic patients. Results. Significant differences among ApN levels according to AER were found in both types of diabetes (F = 8.45, df = 2, P < 0.001). With the progression of albuminuria, ApN increased in type 1 and decreased in type 2 diabetes. Patients with decreased CrCl had higher ApN levels than those with normal CrCl in either type of diabetes (F = 12.7, df = 1, P < 0.001). The best model for ApN (R2 = 0.9002) obtained from stepwise regression in type 1 diabetes included CrCl, BMI, WBC, CRP, and age, while in type 2 diabetes (R2 = 0.2882) it included ppPG, LDL, and UA. Conclusion. ApN behaved differently in relation to albuminuria, increasing with its progression in type 1 diabetes and decreasing in type 2 diabetes. It was however increased in the subgroups with decreased CrCl in both types of diabetes. Albuminuria seems to be more important than renal insufficiency in the definition of ApN levels in type 1 and type 2 diabetes. PMID:26089882

  14. Inverse Levels of Adiponectin in Type 1 and Type 2 Diabetes Are in Accordance with the State of Albuminuria

    Directory of Open Access Journals (Sweden)

    Spomenka Ljubic

    2015-01-01

    Full Text Available Aims. To investigate the behaviour of adiponectin (ApN in patients with type 1 and type 2 diabetic nephropathy. Methods. ApN and inflammatory and other markers of the metabolic syndrome were compared across diabetes types, albumin excretion rate (AER, and creatinine clearance (CrCl categories in 219 type 1 and type 2 diabetic patients. Results. Significant differences among ApN levels according to AER were found in both types of diabetes (F=8.45, df=2, P<0.001. With the progression of albuminuria, ApN increased in type 1 and decreased in type 2 diabetes. Patients with decreased CrCl had higher ApN levels than those with normal CrCl in either type of diabetes (F=12.7, df=1, P<0.001. The best model for ApN (R2=0.9002 obtained from stepwise regression in type 1 diabetes included CrCl, BMI, WBC, CRP, and age, while in type 2 diabetes (R2=0.2882 it included ppPG, LDL, and UA. Conclusion. ApN behaved differently in relation to albuminuria, increasing with its progression in type 1 diabetes and decreasing in type 2 diabetes. It was however increased in the subgroups with decreased CrCl in both types of diabetes. Albuminuria seems to be more important than renal insufficiency in the definition of ApN levels in type 1 and type 2 diabetes.

  15. Cardioprotection of ischemic preconditioning in rats involves upregulating adiponectin.

    Science.gov (United States)

    Wang, Hui; Wu, Wenjing; Duan, Jun; Ma, Ming; Kong, Wei; Ke, Yuannan; Li, Gang; Zheng, Jingang

    2017-02-20

    It has been reported that ischemic preconditioning (IPC) and adiponectin (APN) are cardioprotective in many cardiovascular disorders. However, whether APN mediates the effect of IPC on myocardial injury has not been elucidated. This study was conducted to investigate whether IPC affects myocardial ischemic injury by increasing APN expression. Male adult rats with cardiac knockdowns of APN and its receptors via intramyocardial small-interfering RNA injection were subjected to IPC and then myocardial infarction (MI) at 24 h post-IPC. Globular APN (gAd) was injected at 10 min before MI. APN mRNA and protein levels in myocardium as well as the plasma APN concentration were markedly high at 6 and 12 h after IPC. IPC ameliorated myocardial injury as evidenced by improved cardiac functions and a reduced infarct size. Compared with the control MI group, rats in the IPC + MI group had elevated levels of left ventricular ejection fraction and fractional shortening, and a smaller MI size (PIPC are partially due to upregulation of APN, and provide a further insight into IPC-mediated signaling effects.

  16. Differential gene expression pattern in biopsies with renal allograft pyelonephritis and allograft rejection

    Science.gov (United States)

    Oghumu, Steve; Nori, Uday; Bracewell, Anna; Zhang, Jianying; Bott, Cherri; Nadasdy, Gyongyi M.; Brodsky, Sergey V.; Pelletier, Ronald; Satoskar, Abhay R.; Nadasdy, Tibor; Satoskar, Anjali A.

    2016-01-01

    Differentiating acute pyelonephritis (APN) from acute rejection (AR) in renal allograft biopsies can sometimes be difficult because of overlapping clinical and histologic features, lack of positive urine cultures, and variable response to antibiotics. We wanted to study differential gene expression between AR and APN using biopsy tissue. Thirty-three biopsies were analyzed using NanoString multiplex platform and PCR (6 transplant baseline biopsies, 8 AR, 15 APN [8 culture positive, 7 culture negative], and 4 native pyelonephritis [NP]). Additional 22 biopsies were tested by PCR to validate the results. CXCL9, CXCL10, CXCL11, and IDO1 were the top differentially expressed genes, upregulated in AR. Lactoferrin (LTF) and CXCL1 were higher in APN and NP. No statistically significant difference in transcript levels was seen between culture-positive and culture-negative APN biopsies. Comparing the overall mRNA signature using Ingenuity pathway analysis, interferon-gamma emerged as the dominant upstream regulator in AR and allograft APN, but not in NP (which clustered separately). Our study suggests that chemokine pathways in graft APN may differ from NP and in fact resemble AR, due to a component of alloreactivity, resulting in variable response to antibiotic treatment. Therefore, cautious addition of steroids might help in resistant cases of graft APN. PMID:27352120

  17. Three-month treatment with pioglitazone reduces circulating C1q-binding adiponectin complex to total-adiponectin ratio, without changes in body mass index, in people with type 2 diabetes.

    Science.gov (United States)

    Nakatsuji, Hideaki; Kishida, Ken; Kobayashi, Hironori; Funahashi, Tohru; Shimomura, Iichiro

    2013-01-01

    We measured circulating C1q-binding adiponectin (C1q-APN) levels before and after 3-month treatment with pioglitazone in people with type 2 diabetes. The results indicate 3-month treatment with pioglitazone reduces circulating levels of C1q-APN/total-adiponectin ratio without changes in body mass index.

  18. Differential gene expression pattern in biopsies with renal allograft pyelonephritis and allograft rejection.

    Science.gov (United States)

    Oghumu, Steve; Nori, Uday; Bracewell, Anna; Zhang, Jianying; Bott, Cherri; Nadasdy, Gyongyi M; Brodsky, Sergey V; Pelletier, Ronald; Satoskar, Abhay R; Nadasdy, Tibor; Satoskar, Anjali A

    2016-09-01

    Differentiating acute pyelonephritis (APN) from acute rejection (AR) in renal allograft biopsies can sometimes be difficult because of overlapping clinical and histologic features, lack of positive urine cultures,and variable response to antibiotics. We wanted to study differential gene expression between AR and APN using biopsy tissue. Thirty-three biopsies were analyzed using NanoString multiplex platform and PCR (6 transplant baseline biopsies, 8 AR, 15 APN [8 culture positive, 7 culture negative], and 4 native pyelonephritis [NP]). Additional 22 biopsies were tested by PCR to validate the results. CXCL9, CXCL10, CXCL11, and IDO1 were the top differentially expressed genes, upregulated in AR. Lactoferrin (LTF) and CXCL1 were higher in APN and NP. No statistically significant difference in transcript levels was seen between culture-positive and culture-negative APN biopsies. Comparing the overall mRNA signature using Ingenuity pathway analysis, interferon-gamma emerged as the dominant upstream regulator in AR and allograft APN, but not in NP (which clustered separately). Our study suggests that chemokine pathways in graft APN may differ from NP and in fact resemble AR, due to a component of alloreactivity, resulting in variable response to antibiotic treatment. Therefore, cautious addition of steroids might help in resistant cases of graft APN.

  19. Outcome of the second Medicines Utilisation Research in Africa Group meeting to promote sustainable and appropriate medicine use in Africa.

    Science.gov (United States)

    Massele, Amos; Burger, Johanita; Kalemeera, Francis; Jande, Mary; Didimalang, Thatayaone; Kalungia, Aubrey Chichonyi; Matshotyana, Kidwell; Law, Michael; Malone, Brighid; Ogunleye, Olayinka; Oluka, Margaret; Paramadhas, Bene D; Rwegerera, Godfrey; Zinyowera, Sekesai; Godman, Brian

    2016-11-03

    The second Medicines Utilization Research in Africa (MURIA) group workshop and symposium again brought researchers together from across Africa to improve their knowledge of drug utilization (DU) methodologies and exchange ideas to further progress DU research in Africa. This built on extensive activities from the first conference including workshops and multiple publications. Anti-infectives were again the principal theme for the 2016 symposium following the workshops. This included presentations regarding strategies to improve antibiotic utilization among African countries, such as point-prevalence studies, as well as potential ways to reduce self-purchasing of antibiotics. There were also presentations on antiretrovirals including renal function and the impact of policy changes. Concerns with adherence in chronic treatments as well as drug-drug interactions and their implications were also discussed. The deliberations resulted in a number of agreed activities including joint publications before the next MURIA conference in Namibia in 2017.

  20. Pseudopregnancy induces the expression of hepatocyte nuclear factor-1 beta and its target gene aminopeptidase N in rabbit endometrium via the epithelial promoter

    DEFF Research Database (Denmark)

    Classen-Linke, I; Sjöström, H; Norén, O

    1995-01-01

    The rabbit endometrium is an excellent model system allowing experimental manipulation of aminopeptidase N (APN) mRNA expression in vivo. By RNase mapping and sequencing of cloned PCR-amplified primer-extended RNA, it was demonstrated that endometrial APN expression is directed by the epithelial...... APN promoter and is increased in human-choriogonadotropin-induced pseudopregnancy. Cloning and sequencing of the rabbit APN epithelial promoter revealed conservation of the upstream footprint (UF), hepatocyte nuclear factor-1 (HNF1) and Sp1 elements known to be present in the pig and human promoters...... as well. The pseudopregnancy-induced APN expression was found to be accompanied by a parallel increase in the level of the transcription factor HNF1 beta, whereas a much smaller increase in Sp1 and UF-binding proteins was observed. This indicates that HNF1 beta acts as a switch triggering the pregnancy...

  1. Sistem Agribisnis Dan Kemitraan Petani Ubi Jalar (Studi kasus: Desa Purba Tongah. Kecamatan Purba. Kabupaten Simalungun. Sumatera Utara.)

    OpenAIRE

    Silaban, Nixon H

    2012-01-01

    Penentuan daerah sampel dilakukan secara purposive dan pengambilan sampel dilakukan secara stratified random sampling. Besar petani sampel 30 kepaia keluarga. Metode analisis deskriptif dan koetisien korelasi Rank Spearmen. 950304048

  2. Reduced circulating oxytocin and High-Molecular-Weight adiponectin are risk factors for metabolic syndrome.

    Science.gov (United States)

    Yuan, Guoyue; Qian, Weiyun; Pan, Ruirong; Jia, Jue; Jiang, Dan; Yang, Qichao; Wang, Su; Liu, Yuanxin; Yu, Shuqin; Hu, Hao; Sun, Wenjun; Ye, Jingjing; Mao, Chaoming; Zhuang, Ruo; Zhou, Libin

    2016-07-30

    The neurohypophysial hormone, oxytocin, is involved in the regulation of energy metabolism. Adiponectin (APN) is an adipose tissue-specific serum protein that inversely associates with metabolic syndrome (MetS). High-molecular-weight adiponectin (HMW APN) is considered the active form. In the present study, we aimed to determine the relationships of oxytocin and HMW APN to MetS and investigate whether or not the combination of oxytocin and HMW APN is associated with further metabolic abnormalities compared to each of them alone. A total of 170 subjects (75 with MetS and 95 non-MetS) were enrolled. Anthropometric parameters, oral glucose tolerance test (OGTT), blood lipids, hs-CRP, oxytocin and HMW APN levels were measured. Compared with non-MetS subjects, serum oxytocin and HMW APN levels were significantly lower in subjects with MetS (Poxytocin and high HMW APN levels (high score group), low oxytocin and low HMW APN levels (low score group) and others. Participants in low score group showed the worst metabolic profiles and were more likely to have MetS compared to the other two group. In Spearman rank correlation coefficient, the classification by the combination of oxytocin and HMW APN was significantly correlated with a larger number of metabolic risk factors compared with classification by each of them alone. Individuals with low circulating oxytocin levels coupled with low HMW APN levels were at significantly increased risk of MetS. The combination of both markers would be useful for identifying MetS high risk patients.

  3. Application of the phylogenetic species concept to Wallemia sebi from house dust and indoor air revealed by multi-locus genealogical concordance.

    Directory of Open Access Journals (Sweden)

    Hai D T Nguyen

    Full Text Available A worldwide survey of Wallemia occurring in house dust and indoor air was conducted. The isolated strains were identified as W. sebi and W. muriae. Previous studies suggested that the W. sebi phylogenetic clade contained cryptic species but conclusive evidence was lacking because only the internal transcribed spacer (ITS marker was analyzed. The ITS and four protein-coding genes (MCM7, RPB1, RPB2, and TSR1 were sequenced for 85 isolates. Based on an initial neighbor joining analysis of the concatenated genes, W. muriae remained monophyletic but four clades were found in W. sebi, which we designated as W. sebi clades 1, 2, 3, and 4. We hypothesized that these clades represent distinct phylogenetic species within the Wallemia sebi species complex (WSSC. We then conducted multiple phylogenetic analyses and demonstrated genealogical concordance, which supports the existence of four phylogenetic species within the WSSC. Geographically, W. muriae was only found in Europe, W. sebi clade 3 was only found in Canada, W. sebi clade 4 was found in subtropical regions, while W. sebi clade 1 and 2 were found worldwide. Haplotype analysis showed that W. sebi clades 1 and 2 had multiple haplotypes while W. sebi clades 3 and 4 had one haplotype and may have been under sampled. We describe W. sebi clades 2, 3, and 4 as new species in a companion study.

  4. Analisis Pemasaran Wortel (Studi Kasus : Desa Gajah, Kec. Simpang IV, Kab. Karo, Prop. Sumatera Utara)

    OpenAIRE

    Rahman, Dewi Fauziah

    2012-01-01

    Penentuan daerah penelitian dilakukan secara purposive, pengambilan sampel petani dilakukan secara Stratified Random Sampling dan pengambilan sampel pedagang dilakukan secara Accidental Sampling, dengan jumlah petani sampel 30 kepala keluarga. Metode analisis yang digunakan adalah anal isis deskriptif dan metode Analisis Regresi Berganda. 950304010

  5. Pengukuran Sumber Daya Manusia sebagai Human Capital di PT. Perkebunan Nusantara IV (Persero) Medan

    OpenAIRE

    Mirza, Audy

    2012-01-01

    Human capital merupakan kemampuan yang dimiliki oleh manusia dan bernilai apabila dikelola secara tepat sebagai suatu investasi. Pelatihan dan rekrutmen merupakan investasi yang paling penting. Saat ini, perusahaan telah secara konsisten melakukan pelatihan dan rekrutmen untuk meningkatkan kinerja sumber daya manusianya. Namun, perusahaan tidak mengetahui secara pasti peran atau kontribusi yang diberikan sumber daya manusia terhadap sasaran perusahaan atas program investasi sumber daya manusi...

  6. Advanced Practice Nursing: A Strategy for Achieving Universal Health Coverage and Universal Access to Health

    Science.gov (United States)

    Bryant-Lukosius, Denise; Valaitis, Ruta; Martin-Misener, Ruth; Donald, Faith; Peña, Laura Morán; Brousseau, Linda

    2017-01-01

    ABSTRACT Objective: to examine advanced practice nursing (APN) roles internationally to inform role development in Latin America and the Caribbean to support universal health coverage and universal access to health. Method: we examined literature related to APN roles, their global deployment, and APN effectiveness in relation to universal health coverage and access to health. Results: given evidence of their effectiveness in many countries, APN roles are ideally suited as part of a primary health care workforce strategy in Latin America to enhance universal health coverage and access to health. Brazil, Chile, Colombia, and Mexico are well positioned to build this workforce. Role implementation barriers include lack of role clarity, legislation/regulation, education, funding, and physician resistance. Strong nursing leadership to align APN roles with policy priorities, and to work in partnership with primary care providers and policy makers is needed for successful role implementation. Conclusions: given the diversity of contexts across nations, it is important to systematically assess country and population health needs to introduce the most appropriate complement and mix of APN roles and inform implementation. Successful APN role introduction in Latin America and the Caribbean could provide a roadmap for similar roles in other low/middle income countries. PMID:28146177

  7. Discordant findings of dimercaptosuccinic acid scintigraphy in children with multi-detector row computed tomography-proven acute pyelonephritis

    Directory of Open Access Journals (Sweden)

    Jeongmin Lee

    2011-05-01

    Full Text Available Purpose : The diagnosis of acute pyelonephritis (APN is often difficult, as its clinical and biological manifestations are non-specific in children. If not treated quickly and adequately, however, APN may cause irreversible renal damage, possibly leading to hypertension and chronic renal failure. We were suspecting the diagnostic value of 99mTc-dimercaptosuccinic acid (DMSA scan by experiences and so compared the results of DMSA scan to those of multi-detector row computed tomography (MDCT. Methods : We retrospectively selected and analyzed 81 patients who were diagnosed as APN by MDCT during evaluation of their acute abdomen in emergency room and then received DMSA scan also for the diagnostic work-up of APN after admission. We evaluated the results of imaging studies and compared the diagnostic value of each method by age groups, &lt;2 years (n=45 and ?#242; years (n=36. Results : Among total 81 patients with MDCT-proven APN, DMSA scan was diagnostic only in 55 children (68%, while the remaining 26 children (32% showed false negative normal findings. These 26 patients were predominantly male and most of them, 19 (73.1% were &lt;2 years of age. Conclusion : DMSA scan holds obvious limitation compared to MDCT in depicting acute inflammatory lesions of kidney in children with APN, especially in early childhood less than 2 years of age. MDCT showed hidden lesions of APN, those were undetectable through DMSA scan in children.

  8. Regulation of the cardioprotective adiponectin and its receptor AdipoR1 by salt.

    Science.gov (United States)

    Arnold, Nicholas; Mahmood, Abuzar; Ramdas, Maya; Ehlinger, Paul P; Pulakat, Lakshmi

    2017-03-01

    Both circulating adiponectin (APN) and cardiac APN exert cardioprotective effects and improve insulin sensitivity and mitochondrial function. Low circulating APN serves as a biomarker for cardiovascular risk. Ablation of adiponectin receptor 1 (AdipoR1) causes myocardial mitochondrial dysfunction. Although high salt intake is a contributor to cardiovascular disease, how it modulates the expression of APN or AdipoR1 in cardiomyocytes is not known. We report that APN mRNA expression was attenuated in a dose-dependent manner in mouse cardiomyocyte cell line HL-1 exposed to salt concentrations ranging from 0.75% to 1.5% for 12 h. High-salt exposure (0.88% and 1.25% for 12 h) also suppressed APN and AdipoR1 protein expression significantly in rat cardiac muscle H9c2 cells. Co-immunostaining for AdipoR1 and mitochondrial complex 1 indicated that AdipoR1 may be co-localized with mitochondria. These data show for the first time that high salt is an important suppressor of cardiovascular protective APN and AdipoR1.

  9. Effects of Activity-Based Personalized Nutrition Education on Dietary Behaviors and Blood Parameters in Middle-Aged and Older Type 2 Diabetes Korean Outpatients

    Science.gov (United States)

    2016-01-01

    This study aimed to compare the effects of activity-based personalized nutrition education (APNE) with a general instruction for diabetes (control, CTRL) in middle-aged and older Korean outpatients with type 2 diabetes. After an initial screening, 70 subjects were randomly assigned to APNE (n = 37) or CTRL (n = 33) group. APNE considered each patient’s anthropometry, blood chemistry data, and dietary habits in addition to planning meal choices with the aid of registered dietitians. After 3 months, dietary behavior, food intake, and anthropometric and blood measurement results were evaluated. Fasting blood glucose, 2-hour postprandial blood glucose, and glycated hemoglobin levels decreased in the APNE group (n = 33) but not in the CTRL group (n = 23). In the APNE group, the meal intervals and number of days of consuming high-fat food were decreased, while the number of days following a meal plan and balanced diet that entailed consuming fruits, vegetables, and healthy food was increased. A lower consumption of carbohydrates, saccharides, grains, and tuber crops and a higher protein, pulses, and fat-derived calorie intake compared with the initial values were observed in the APNE group. In contrast, only the number of days following the meal plan and balanced diet was increased in the CRTL group, without significantly changing the individual macronutrient-derived calorie intake. The APNE approach appeared to effectively educate outpatients with type 2 diabetes about changing their dietary behavior and food intake and improving the clinical parameters related to diabetic conditions. PMID:27812513

  10. Advanced Practice Nursing: A Strategy for Achieving Universal Health Coverage and Universal Access to Health

    Directory of Open Access Journals (Sweden)

    Denise Bryant-Lukosius

    Full Text Available ABSTRACT Objective: to examine advanced practice nursing (APN roles internationally to inform role development in Latin America and the Caribbean to support universal health coverage and universal access to health. Method: we examined literature related to APN roles, their global deployment, and APN effectiveness in relation to universal health coverage and access to health. Results: given evidence of their effectiveness in many countries, APN roles are ideally suited as part of a primary health care workforce strategy in Latin America to enhance universal health coverage and access to health. Brazil, Chile, Colombia, and Mexico are well positioned to build this workforce. Role implementation barriers include lack of role clarity, legislation/regulation, education, funding, and physician resistance. Strong nursing leadership to align APN roles with policy priorities, and to work in partnership with primary care providers and policy makers is needed for successful role implementation. Conclusions: given the diversity of contexts across nations, it is important to systematically assess country and population health needs to introduce the most appropriate complement and mix of APN roles and inform implementation. Successful APN role introduction in Latin America and the Caribbean could provide a roadmap for similar roles in other low/middle income countries.

  11. Comparison of acute lobar nephronia and acute pyelonephritis in children: a single-center clinical analysis in southern taiwan.

    Science.gov (United States)

    Chen, Wan-Ling; Huang, I-Fei; Wang, Jiun-Ling; Hung, Chih-Hsin; Huang, Jer-Shyung; Chen, Yao-Shen; Lee, Susan Shin-Jung; Hsieh, Kai-Sheng; Tang, Chia-Wan; Chien, Jen-Hung; Chiou, Yee-Hsuan; Cheng, Ming-Fang

    2015-06-01

    Patients with acute lobar nephronia (ALN) require a longer duration of antimicrobial treatment than those with acute pyelonephritis (APN), and ALN is associated with renal scarring. The aim of this study was to provide an understanding of ALN by comparing the clinical features of pediatric patients with ALN and APN. We enrolled all of the patients with ALN (confirmed by computed tomography) admitted to our hospital from 1999 to 2012 in the ALN group. In addition, each patient diagnosed with APN who was matched for sex, age, and admission date to each ALN patient was enrolled in the APN group. The medical charts of patients in these two groups were retrospectively reviewed and analyzed for comparison. The fever duration after hospitalization in the ALN group and the APN group were 4.85 ± 2.33 days and 2.30 ± 1.47 days respectively. The microbiological distributions and the majority of susceptibilities were similar in the ALN and APN groups. The majority of clinical manifestations are nonspecific and unreliable for the differentiation of ALN and APN. The patients with ALN were febrile for longer after antimicrobial treatment, had more nausea/vomiting symptoms, higher neutrophil count, bandemia, and C-reactive protein (CRP) levels, and lower platelet count (all p  5 days after antimicrobial treatment. Copyright © 2014. Published by Elsevier B.V.

  12. Reduction Levels and the Effects of High-Molecular-Weight Adiponectin via AMPK/eNOS in Chinese Type 2 Diabetes.

    Science.gov (United States)

    Lian, K; Guo, X; Huang, Q; Tan, Z; Xia, C; Wang, R; Yi, F; Li, W; Liu, J; Li, Y; Guo, S; Wang, H; Ji, Q; Tao, L

    2016-10-01

    Aim: This study was to investigate the change of high-molecula-weight (HMW) adiponectin (APN) isoform, the association between type 2 diabetes mellitus (T2DM) and HMW APN isoform, the variation of Disulfide-bond A oxidoreductase-like protein (DsbA-L), the effect of HMW APN isoform on AMP-dependent protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) in Chinese T2DM. Method: 169 patients aged at (48.7±9.4) years and 107 healthy control subjects aged at (42.6±7.8) years took part in this study. Anthropometric measures of the characters were assayed and different APN isoforms, DsbA-L, AMPK and eNOS levels were determined. Results: Ln(sRAGE) and Ln(Adiponectin) were significantly lower and significantly higher for the other characteristics in T2DM. Ln(Adiponectin) was negatively and significantly correlated with WHR, Ln(triglycerides), fasting plasma glucose, HbA1c (%) in control subjects and T2DM patients. Plasma and adipose tissue total APN and HMW APN were significantly reduced in newly diagnosed T2DM patients. DsbA-L was markedly down-regulated in diabetic adipose tissue. HMW APN caused significant decreases in AMPK and eNOS phosphorylation levels of human umbilical vein endothelial cells (HUVECs). Conclusions: Our results demonstrated that total APN levels was closely related to the risk of T2DM and HMW APN reduction was involved in the diabetic vascular AMPK/eNOS signal pathway. The findings will provide insight into novel therapeutic approaches for reducing the elevated cardiovascular risk associated with T2DM. © Georg Thieme Verlag KG Stuttgart · New York.

  13. Adiponectin attenuates angiotensin II-induced vascular smooth muscle cell remodeling through nitric oxide and the RhoA/ROCK pathway.

    Directory of Open Access Journals (Sweden)

    Wared eNour-Eldine

    2016-04-01

    Full Text Available INTRODUCTION: Adiponectin (APN, an adipocytokine, exerts protective effects on cardiac remodeling, while angiotensin II (Ang II induces hypertension and vascular remodeling. The potential protective role of APN on the vasculature during hypertension has not been fully elucidated yet. Here, we evaluate the molecular mechanisms of the protective role of APN in the physiological response of the vascular wall to Ang II.METHODS AND RESULTS: Rat aortic tissues were used to investigate the effect of APN on Ang II-induced vascular remodeling and hypertrophy. We investigated whether nitric oxide (NO, the RhoA/ROCK pathway, actin cytoskeleton remodeling, and reactive oxygen species (ROS mediate the anti-hypertrophic effect of APN. Ang II-induced protein synthesis was attenuated by pre-treatment with APN, NO donor (SNAP, or cGMP. The hypertrophic response to Ang II was associated with a significant increase in RhoA activation and vascular force production, which were prevented by APN and SNAP. NO was also associated with inhibition of Ang II-induced phosphorylation of cofilin. In addition, immunohistochemistry revealed that 24 hr Ang II treatment increased the F- to G-actin ratio, an effect that was inhibited by SNAP. Ang II-induced ROS formation and upregulation of p22phox mRNA expression were inhibited by APN and NO. Both compounds failed to inhibit Nox1 and p47phox expression. CONCLUSIONS: Our results suggest that the anti-hypertrophic effects of APN are due, in part, to NO-dependent inhibition of the RhoA/ROCK pathway and ROS formation.

  14. Intriguing interplay between feline infectious peritonitis virus and its receptors during entry in primary feline monocytes.

    Science.gov (United States)

    Van Hamme, Evelien; Desmarets, Lowiese; Dewerchin, Hannah L; Nauwynck, Hans J

    2011-09-01

    Two potential receptors have been described for the feline infectious peritonitis virus (FIPV): feline aminopeptidase N (fAPN) and feline dendritic cell-specific intercellular adhesion molecule grabbing non-integrin (fDC-SIGN). In cell lines, fAPN serves as a receptor for serotype II, but not for serotype I FIPV. The role of fAPN in infection of in vivo target cells, monocytes, is not yet confirmed. Both serotype I and II FIPVs use fDC-SIGN for infection of monocyte-derived cells but how is not known. In this study, the role of fAPN and fDC-SIGN was studied at different stages in FIPV infection of monocytes. First, the effects of blocking the potential receptor(s) were studied for the processes of attachment and infection. Secondly, the level of co-localization of FIPV and the receptors was determined. It was found that FIPV I binding and infection were not affected by blocking fAPN while blocking fDC-SIGN reduced FIPV I binding to 36% and practically completely inhibited infection. Accordingly, 66% of bound FIPV I particles co-localized with fDC-SIGN. Blocking fAPN reduced FIPV II binding by 53% and infection by 80%. Further, 60% of bound FIPV II co-localized with fAPN. fDC-SIGN was not involved in FIPV II binding but infection was reduced with 64% when fDC-SIGN was blocked. In conclusion, FIPV I infection of monocytes depends on fDC-SIGN. Most FIPV I particles already interact with fDC-SIGN at the plasma membrane. For FIPV II, both fAPN and fDC-SIGN are involved in infection with only fAPN playing a receptor role at the plasma membrane.

  15. Effect of adiponectin deficiency on intestinal damage and hematopoietic responses of mice exposed to gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Ponemone, Venkatesh; Fayad, Raja; Gove, Melissa E.; Pini, Maria [Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, IL 60612 (United States); Fantuzzi, Giamila, E-mail: giamila@uic.edu [Department of Kinesiology and Nutrition, University of Illinois at Chicago, Chicago, IL 60612 (United States)

    2010-08-07

    Adiponectin (APN) is an adipose tissue-derived cytokine that regulates insulin sensitivity and inflammation. It is also involved in modulation of cell proliferation by binding to various growth factors. Based on its known effects in modulating cell proliferation and oxidative stress, APN may potentially be involved in regulating tissue damage and repair following irradiation. Adiponectin KO mice and their WT littermates were exposed to a single whole-body dose of 3 or 6 Gy gamma radiation. Radiation-induced alterations were studied in jejunum, blood, bone marrow and thymus at days 1 and 5 post-irradiation and compared with sham-irradiated groups. In WT mice, irradiation did not significantly alter serum APN levels while inducing a significant decrease in serum leptin. Irradiation caused a significant reduction in thymocyte cellularity, with concomitant decrease in CD4{sup +}, CD8{sup +} and CD4{sup +}CD8{sup +} T cell populations, with no significant differences between WT and APN KO mice. Irradiation resulted in a significantly higher increase in the frequency of micronucleated reticulocytes in the blood of APN KO compared with WT mice, whereas frequency of micronucleated normochromatic erythrocytes in the bone marrow at day 5 was significantly higher in WT compared with APN KO mice. Finally, irradiation induced similar alterations in villus height and crypt cell proliferation in the jejunum of WT and APN KO mice. Jejunum explants from sham-irradiated APN KO mice produced higher levels of IL-6 compared with tissue from WT animals, but the difference was no longer apparent following irradiation. Our data indicate that APN deficiency does not play a significant role in modulating radiation-induced gastrointestinal injury in mice, while it may participate in regulation of damage to the hematopoietic system.

  16. Advanced practice nursing in performing arts health care.

    Science.gov (United States)

    Weslin, Anna T; Silva-Smith, Amy

    2010-06-01

    Performing arts medicine is a growing health care profession specializing in the needs of performing artists. As part of the performing arts venue, the dancer, a combination of athlete and artist, presents with unique health care needs requiring a more collaborative and holistic health care program. Currently there are relatively few advanced practice nurses (APNs) who specialize in performing arts health care. APNs, with focus on collaborative and holistic health care, are ideally suited to join other health care professionals in developing and implementing comprehensive health care programs for the performing artist. This article focuses on the dancer as the client in an APN practice that specializes in performing arts health care.

  17. Adiponectin attenuates lung fibroblasts activation and pulmonary fibrosis induced by paraquat.

    Directory of Open Access Journals (Sweden)

    Rong Yao

    Full Text Available Pulmonary fibrosis is one of the most common complications of paraquat (PQ poisoning, which demands for more effective therapies. Accumulating evidence suggests adiponectin (APN may be a promising therapy against fibrotic diseases. In the current study, we determine whether the exogenous globular APN isoform protects against pulmonary fibrosis in PQ-treated mice and human lung fibroblasts, and dissect the responsible underlying mechanisms. BALB/C mice were divided into control group, PQ group, PQ + low-dose APN group, and PQ + high-dose APN group. Mice were sacrificed 3, 7, 14, and 21 days after PQ treatment. We compared pulmonary histopathological changes among different groups on the basis of fibrosis scores, TGF-β1, CTGF and α-SMA pulmonary content via Western blot and real-time quantitative fluorescence-PCR (RT-PCR. Blood levels of MMP-9 and TIMP-1 were determined by ELISA. Human lung fibroblasts WI-38 were divided into control group, PQ group, APN group, and APN receptor (AdipoR 1 small-interfering RNA (siRNA group. Fibroblasts were collected 24, 48, and 72 hours after PQ exposure for assay. Cell viability and apoptosis were determined via Kit-8 (CCK-8 and fluorescein Annexin V-FITC/PI double labeling. The protein and mRNA expression level of collagen type III, AdipoR1, and AdipoR2 were measured by Western blot and RT-PCR. APN treatment significantly decreased the lung fibrosis scores, protein and mRNA expression of pulmonary TGF-β1, CTGF and α-SMA content, and blood MMP-9 and TIMP-1 in a dose-dependent manner (p<0.05. Pretreatment with APN significantly attenuated the reduced cell viability and up-regulated collagen type III expression induced by PQ in lung fibroblasts, (p<0.05. APN pretreatment up-regulated AdipoR1, but not AdipoR2, expression in WI-38 fibroblasts. AdipoR1 siRNA abrogated APN-mediated protective effects in PQ-exposed fibroblasts. Taken together, our data suggests APN protects against PQ-induced pulmonary fibrosis in a

  18. Department of the Navy Justification of Estimates for Fiscal Year 1985. Submitted to Congress February 1984. Procurement, Aircraft Procurement, Navy

    Science.gov (United States)

    1984-02-01

    1979 FY 1981 FY 198? FY 1983 FT 1Q84 FT 1985 _ Cost (M Cost _q• Cost _%I Cost M Cost t Cost APN-5 $600 $934 1 $199 $946 60 $3,302 55 $6.879 O&WN Install...require verification. ProJect Financial Plan: FY 1q84 FY 1985 FY 1986 FY 1987 FY 1988 TOTAL Q cost StU Cost zt Cost zt Cost 2__ Cost .Qt Cost APN-5 2...provide a parallel altimeter readout in the front cockpit off of the current AN/APN-171 system. ( ProJect Financial Plan: FT 1q84 FT 1985 FT 1986

  19. T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides

    DEFF Research Database (Denmark)

    Larsen, S L; Pedersen, L O; Buus, S;

    1996-01-01

    the exopeptidase Aminopeptidase N (APN, CD13) as one of the enzymes involved in the observed cell-surface antigen processing. The NH2-terminal end of the longer peptide could, even while bound to major histocompatibility complex (MHC) class II molecules, be digested by APN with dramatic consequences for T cell...... antigen recognition. This could be demonstrated both in cell-free systems using purified reagents and in cellular systems. Thus, MHC class II and APN may act in concert to generate the final T cell epitopes....

  20. The Progress of Research on Determination Methods for Alkylphenol Ethoxylates in Textiles%纺织品中烷基酚聚氧乙烯醚检测方法的研究进展

    Institute of Scientific and Technical Information of China (English)

    赵海浪; 诸佩菊; 吴东晓

    2013-01-01

    烷基酚聚氧乙烯醚(APnEO)是一类非离子表面活性剂,在纺织品产业中应用广泛.本文概述了APnEO的来源及其危害,介绍GC-MS、HPLC、HPLC-NMR、HPLC-MS法检测APnEO的研究情况,并对纺织品中烷基酚聚氧乙烯醚检测方法进行展望.

  1. Plasma Adiponectin, clinical factors, and patient outcomes during the acute respiratory distress syndrome.

    Directory of Open Access Journals (Sweden)

    Allan J Walkey

    Full Text Available Adiponectin (APN is an anti-inflammatory hormone derived from adipose tissue that attenuates acute lung injury in rodents. In this study, we investigated the association between circulating APN and outcomes among patients with acute respiratory distress syndrome (ARDS.We performed a retrospective cohort study using data and plasma samples from participants in the multicenter ARDS Network Fluid and Catheter Treatment Trial.Plasma APN concentrations were measured in 816 (81.6% trial participants at baseline and in 568 (56.8% subjects at both baseline and day 7 after enrollment. Clinical factors associated with baseline APN levels in multivariable-adjusted models included sex, body mass index, past medical history of cirrhosis, and central venous pressure (model R2 = 9.7%. We did not observe an association between baseline APN and either severity of illness (APACHE III or extent of lung injury (Lung Injury Score. Among patients who received right heart catheterization (n = 384, baseline APN was inversely related to mean pulmonary artery pressure (β = -0.015, R2 1.5%, p = 0.02; however, this association did not persist in multivariable models (β = -0.009, R2 0.5%, p = 0.20. Neither baseline APN levels [HR per quartile1.04 (95% CI 0.91-1.18, p = 0.61], nor change in APN level from baseline to day 7 [HR 1.04 (95% CI 0.89-1.23, p = 0.62] were associated with 60 day mortality in Cox proportional hazards regression models. However, subgroup analysis identified an association between APN and mortality among patients who developed ARDS from extra-pulmonary etiologies [HR per quartile 1.31 (95% CI 1.08-1.57]. APN levels did not correlate with mortality among patients developing ARDS in association with direct pulmonary injury [HR 0.96 (95% CI 0.83-1.13], pinteraction = 0.016.Plasma APN levels did not correlate with disease severity or mortality in a large cohort of patients with ARDS. However, higher APN levels were

  2. Identifikasi Secara Serologi Galur Virus Flu Burung Subtipe H5N1 Clade 2.1.3 dan Clade 2.3.2 pada Ayam Petelur (SEROLOGICAL IDENTIFICATION OF AVIAN INFLUENZA STRAIN VIRUS SUBTYPE H5N1 CLADE 2.1.3 AND CLADE 2.3.2 FROM LAYER

    Directory of Open Access Journals (Sweden)

    Aprilia Kusumastuti

    2015-10-01

    Full Text Available The aim of the study was to know avian influenza (AI infection in field by using serology test in threemarketing area of AI vaccines. Haemagglutination inhibition methode was used in this test. There werefour antigen strains of AI subtype H5N1 clade 2.1.3 (AIstrainA/Chicken/West Java/PWT-WIJ/2006, AIstrain A/Chicken/Garut/BBVW-223/2007, AI strain A/Chicken/West Java-Nagrak/30/2007, and AI strainA/Chicken/Pekalongan/BBVW-208/2007 and 2 antigen strains of AI subtype H5N1 clade 2.3.2 (AI strainA/duck/Sukoharjo/BBVW-1428-9/2012 and AI strain A/duck/Sleman/BBVW-1463-10/2012 was used inthis study for HI test. The result presents that 93,33% chicken farms in three marketing area of PT. SanbioLaboratories have positive antibody titre to AI subtype H5N1 clade 2.1.3. This titre may be obtained fromAI clade 2.1.3 vaccination. From 15 samples, 92,86% are positive to AI subtype H5N1 clade 2.3.2A/duck/Sukoharjo/BBVW-1428-9/2012 and 92,31% are positive to A/duck/Sleman/BBVW-1463-10/2012 evenwithout AI clade 2.3.2 vaccination. This antibody titre may be obtained from AI clade 2.1.3 vaccine crossprotection or field infection.

  3. 家蚕氨肽酶N家族基因在5龄幼虫中肠组织的表达分析%Expression Analysis of Aminopeptidase N Family Genes in Midgut Tissue of the Fifth Instar Bombyx mori Larvae

    Institute of Scientific and Technical Information of China (English)

    王猛; 程晨; 郝碧芳; 徐安英; 沈兴家; 黄金山

    2013-01-01

    Aminopeptidase N (APN) is an enzyme that readily hydrolyzes protein or neutral amino acids at the N-terminal of an oligopeptide.It is mainly distributed in the brush border membrane vesicle of midgut epithelial cells of lepidopterous insects and is the important receptor of Bacillus thuringiensis crystal toxins (Cry).To investigate expression patterns of APN family genes,Real-time PCR was employed to analyze the differential expression of APN family genes in larva migut tissue of different silkworm (Bombyx mori) varieties and the expression level of various family members in larva midgut tissue of the same silkworm variety.The results showed that APN family genes were expressed in larva midgut of all tested silkworm varieties.The expression level of APN genes were significantly different between silkworm varieties having different voltinisms (P <0.05).However,there was little difference between silkworm varieties having the same voltinism (P >0.05).The expression levels of APN2,APN4 and APN5 genes were relatively higher in larva midgut tissue of the same silkworm variety,whereas those of APN1 and APN3 were relatively lower.The obtained results would facilitate further study on functioning mechanism of silkworm APN family genes,and provide theoretical basis for breeding silkworm strains resistant to Bacillus thuringiensis.%氨肽酶N(aminopeptidase N,APN)是一种偏好水解蛋白质或寡肽N端中性氨基酸的酶,在鳞翅目昆虫中主要分布于中肠上皮细胞的刷状缘囊膜上,是苏云金芽孢杆菌(Bacillus thuringiensis,Bt)伴孢晶体(Cry)毒素的重要受体蛋白.为了研究家蚕APN家族基因的表达特征,运用Real-time PCR技术检测分析该家族基因在不同家蚕品种幼虫中肠组织的表达差异以及同一家蚕品种幼虫中肠组织中该家族基因各个成员的表达丰度.在所有供试家蚕品种的幼虫中肠组织均可检测到APN家族基因的表达,但不同化性家蚕品种间APN基因的表

  4. Estudo comparativo das seqüências rápidas ponderadas em T2, utilizando-se sincronização respiratória, apnéia, supressão de gordura, bobina de corpo e bobina de sinergia para a avaliação do fígado pela ressonância magnética

    OpenAIRE

    2003-01-01

    OBJETIVO: Comparar, qualitativa e quantitativamente, as imagens de ressonância magnética do fígado, ponderadas em T2, utilizando-se seqüências rápidas, diferenciadas pela técnica de controle respiratório, pela utilização de supressão de gordura e pelo tipo de bobina de radiofreqüência. MATERIAIS E MÉTODOS: Estudo prospectivo em 71 pacientes consecutivos, sendo realizadas seis seqüências para comparação: 1) supressão de gordura com sincronização respiratória e bobina de corpo; 2) supressão de ...

  5. 76 FR 2710 - Dell Products LP, Winston-Salem (WS-1) Division, Including On-Site Leased Workers From Adecco...

    Science.gov (United States)

    2011-01-14

    ... workers from Staffing Solutions, South East, and Omi Resources and Recovery, SecurAmerica, LLC, Industrial... Leased Workers From Adecco, Spherion, Patriot Staffing, Manpower, Teksystems, APN, ICONMA, Staffing Solutions, South East, Omni Resources and Recovery, Securamerica, LLC, Industrial Distribution Group...

  6. Data of evolutionary structure change: 1APNB-2GDFC [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1APNB-2GDFC 1APN 2GDF B C ADTIVAVELDTYPN------SYPHIGIDIKSVRSKKTAK...WNMQNGKVGTAHIIYNSVDKRLSAVVSYPNADSATVSYDVDLDNVLPEWVRVGLSASTGLYKETNTILSWSFTSKLKSNSTHETNALHFMFNQFSKDQKDLILQGDAT...TGTDGNLELTRVQGSSVGRALFYAPVHIWESSAVVASFEATFTFLIKSPDSHPADGIAFFISNIDSSIPSGSTGRLLGLFPDAN ADTIVAVELDSYPNTDIGD...GLSATTGLYKETNTILSWSFTSKLKTE-----NSLHFSFHKFSQNPKDLILQGDAFTDSDGNLELTKVQGNSVGRALFYAPVHIWEKSAVVASFDATFTFLIKSPDRE...E EEEEEE EEEEEEEEEEEE EEEEEEEEEE GGG 0 2GD

  7. The coronavirus transmissible gastroenteritis virus causes infection after receptor-mediated endocytosis and acid-dependent fusion with an intracellular compartment

    DEFF Research Database (Denmark)

    Hansen, Gert Helge; Delmas, B; Besnardeau, L;

    1998-01-01

    adsorption to the pAPN-MDCK cells. TGEV was also observed in endocytic pits and apical vesicles after 3 to 10 min of incubation at 38 degrees C. The number of pits and apical vesicles was increased by the TGEV incubation, indicating an increase in endocytosis. After 10 min of incubation, a distinct TGEV......-pAPN-containing population of large intracellular vesicles, morphologically compatible with endosomes, was found. A higher density of pAPN receptors was observed in the pits beneath the virus particles than in the surrounding plasma membrane, indicating that TGEV recruits pAPN receptors before endocytosis. Ammonium chloride...... and bafilomycin A1 markedly inhibited the TGEV infection as judged from virus production and protein biosynthesis analyses but did so only when added early in the course of the infection, i.e., about 1 h after the start of endocytosis. Together our results point to an acid intracellular compartment as the site...

  8. Significance of adiponectin in the risk of coronary lesions in patients with impaired glucose regulation

    Institute of Scientific and Technical Information of China (English)

    黄珊

    2013-01-01

    Objective To investigate the association of impaired glucose regulation and adiponectin(APN) with the clinical severity of coronary lesions. Methods A total of 210 cases of suspected coronary heart disease were examined

  9. Sequence Classification: 893347 [

    Lifescience Database Archive (English)

    Full Text Available ating agents; controls spontaneous mutations; Apn1p || http://www.ncbi.nlm.nih.gov/protein/6322735 ... ...inic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkyl

  10. The clinical and imaging presentation of acute "non complicated" pyelonephritis: A new profile for an ancient disease

    Directory of Open Access Journals (Sweden)

    Piccoli Giorgina

    2011-12-01

    Full Text Available Abstract Background Acute pyelonephritis (APN is differently defined according to imaging or clinical criteria. In adults information on the relationship between imaging and clinical data is lacking. Our study was aimed at analysing the relationship between the clinical and imaging presentation of APN, defined according to imaging criteria (parenchymal involvement at MR or CT scan. Methods All consecutive patients hospitalized for "non-complicated" APN were considered (June 2005-December 2009. Clinical, biochemical and imaging data at hospitalization were analyzed by univariate and logistic regression analysis. Results There were 119 patients, all females, median age 32 years (15-72. At hospitalization, inflammatory markers were elevated (CRP median: 12.1 mg/dL, normal Conclusions APN is a protean disease. In the absence of strict correlation with clinical or biochemical markers, imaging studies are required to assess the severity of kidney involvement.

  11. The outcome of interprofessional education: Integrating communication studies into a standardized patient experience for advanced practice nursing students.

    Science.gov (United States)

    Defenbaugh, Nicole; Chikotas, Noreen E

    2016-01-01

    The purpose of this qualitative study was to examine the impact of standardized patient experiences (SPE) in the education of the Advanced Practice Nurse (APN). The education of the APN requires educators to make every attempt to promote competency in the areas of communication and clinical-decision making. SPE programs have been found to improve the interpersonal, problem solving, and critical thinking skills of nursing students. For this research twenty-nine APN students participated in SPEs over the course of two semesters. Fifteen student volunteers of those 29 participants were then interviewed three months after the experience. Results revealed that having an expert in the field of communication studies increased awareness of communication skills and how to improve nurse-patient encounters in the clinical setting. The interprofessional collaboration during the SPEs assisted in facilitating the application of learned communication skills into patient-centered care of the APN student.

  12. Evolving role of adiponectin in cancer-controversies and update

    Institute of Scientific and Technical Information of China (English)

    Arnav Katira; Peng H Tan

    2016-01-01

    Adiponectin (APN), an adipokine produced by adipocytes, has been shown to have a critical role in the pathogenesis of obesity-associated malignancies. Through its receptor interactions, APN may exert its anti-carcinogenic effects including regulating cell survival, apoptosis and metastasis via a plethora of signalling pathways. Despite the strong evidence supporting this notion, some work may indicate otherwise. Our review addresses all controversies critically. On the whole, hypoadiponectinaemia is associated with increased risk of several malignancies and poor prognosis. In addition, various genetic polymorphisms may predispose individuals to increased risk of obesity-associated malignancies. We also provide an updated summary on therapeutic interventions to increase APN levels that are of key interest in this field. To date efforts to manipulate APN levels have been promising, but much work remains to be done.

  13. Analysis of the mechanism of antibody-dependent enhancement of feline infectious peritonitis virus infection: aminopeptidase N is not important and a process of acidification of the endosome is necessary.

    Science.gov (United States)

    Takano, Tomomi; Katada, Yukari; Moritoh, Saiko; Ogasawara, Mika; Satoh, Kumi; Satoh, Ryoichi; Tanabe, Maki; Hohdatsu, Tsutomu

    2008-04-01

    Infection of the monocyte/macrophage lineage with feline infectious peritonitis virus (FIPV) is enhanced in the presence of anti-FIPV antibodies (antibody-dependent enhancement or ADE). We investigated the following unclear points concerning ADE of FIPV infection: (i) involvement of the virus receptor, feline aminopeptidase N (fAPN), in ADE activity in FIPV infection; (ii) necessity of acidification of the endosome in cellular invasion of FIPV. Virus receptor-blocking experiments using anti-fAPN antibodies at 4 or 37 degrees C and experiments using fAPN-negative U937 cells revealed that fAPN is not involved in ADE of FIPV infection. Experiments using lysosomotropic agents clarified that acidification of the endosome is necessary for cellular invasion by FIPV, regardless of the presence or absence of antibodies. These findings may be very important for understanding the mechanism of ADE of FIPV infection.

  14. Evolution of Anabaenopeptin Peptide Structural Variability in the Cyanobacterium Planktothrix

    Science.gov (United States)

    Entfellner, Elisabeth; Frei, Mark; Christiansen, Guntram; Deng, Li; Blom, Jochen; Kurmayer, Rainer

    2017-01-01

    Cyanobacteria are frequently involved in the formation of harmful algal blooms wherein, apart from the toxic microcystins, other groups of bioactive peptides are abundant as well, such as anabaenopeptins (APs). The APs are synthesized nonribosomally as cyclic hexapeptides with various amino acids at the exocyclic position. We investigated the presence and recombination of the AP synthesis gene cluster (apnA-E) through comparing 125 strains of the bloom-forming cyanobacterium Planktothrix spp., which were isolated from numerous shallow and deep water habitats in the temperate and tropical climatic zone. Ten ecologically divergent strains were purified and genome sequenced to compare their entire apnA-E gene cluster. In order to quantify apn gene distribution patterns, all the strains were investigated by PCR amplification of 2 kbp portions of the entire apn gene cluster without interruption. Within the 11 strains assigned to P. pseudagardhii, P. mougeotii, or P. tepida (Lineage 3), neither apnA-E genes nor remnants were observed. Within the P. agardhii/P. rubescens strains from shallow waters (Lineage 1, 52 strains), strains both carrying and lacking apn genes occurred, while among the strains lacking the apnA-E genes, the presence of the 5′end flanking region indicated a gene cluster deletion. Among the strains of the more derived deep water ecotype (Lineage 2, 62 strains), apnA-E genes were always present. A high similarity of apn genes of the genus Planktothrix when compared with strains of the genus Microcystis suggested its horizontal gene transfer during the speciation of P. agardhii/P. rubescens. Genetic analysis of the first (A1-) domain of the apnA gene, encoding synthesis of the exocyclic position of the AP molecule, revealed four genotype groups that corresponded with substrate activation. Groups of genotypes were either related to Arginine only, the coproduction of Arginine and Tyrosine or Arginine and Lysine, or even the coproduction of Arginine

  15. Adiponectin Upregulates MiR-133a in Cardiac Hypertrophy through AMPK Activation and Reduced ERK1/2 Phosphorylation.

    Directory of Open Access Journals (Sweden)

    Ying Li

    Full Text Available Adiponectin and miR-133a are key regulators in cardiac hypertrophy. However, whether APN has a potential effect on miR-133a remains unclear. In this study, we aimed to investigate whether APN could regulate miR-133a expression in Angiotensin II (Ang II induced cardiac hypertrophy in vivo and in vitro. Lentiviral-mediated adiponectin treatment attenuated cardiac hypertrophy induced by Ang II infusion in male wistar rats as determined by reduced cell surface area and mRNA levels of atrial natriuretic peptide (ANF and brain natriuretic peptide (BNP, also the reduced left ventricular end-diastolic posterior wall thickness (LVPWd and end-diastolic interventricular septal thickness (IVSd. Meanwhile, APN elevated miR-133a level which was downregulated by Ang II. To further investigate the underlying molecular mechanisms, we treated neonatal rat ventricular myocytes (NRVMs with recombinant rat APN before Ang II stimulation. Pretreating cells with recombinant APN promoted AMP-activated protein kinase (AMPK phosphorylation and inhibited ERK activation. By using the inhibitor of AMPK or a lentiviral vector expressing AMPK short hairpin RNA (shRNA cancelled the positive effect of APN on miR-133a. The ERK inhibitor PD98059 reversed the downregulation of miR-133a induced by Ang II. These results indicated that the AMPK activation and ERK inhibition were responsible for the positive effect of APN on miR-133a. Furthermore, adiponectin receptor 1 (AdipoR1 mRNA expression was inhibited by Ang II stimulation. The positive effects of APN on AMPK activation and miR-133a, and the inhibitory effect on ERK phosphorylation were inhibited in NRVMs transfected with lentiviral AdipoR1shRNA. In addition, APN depressed the elevated expression of connective tissue growth factor (CTGF, a direct target of miR-133a, through the AMPK pathway. Taken together, our data indicated that APN reversed miR-133a levels through AMPK activation, reduced ERK1/2 phosphorylation in

  16. An organic thin film photodiode as a portable photodetector for the detection of alkylphenol polyethoxylates by a flow fluorescence-immunoassay on magnetic microbeads in a microchannel.

    Science.gov (United States)

    Ishimatsu, Ryoichi; Naruse, Azusa; Liu, Rong; Nakano, Koji; Yahiro, Masayuki; Adachi, Chihaya; Imato, Toshihiko

    2013-12-15

    An organic thin film photodiode (OPD) was successfully employed as a portable photodetector in a competitive enzyme-linked immunosorbent assay (ELISA) of a class of nonionic surfactants, namely alkylphenol polyethoxylates (APnEOs) which are an environmental pollutant. Microbeads that were chemically immobilized with an anti-APnEOs antibody were used in the assay. The OPD consisted of a layer of copper phthalocyanine (CuPc), C60 and a second layer of bathocuproine (BCP) with a bulk heterojunction composed of CuPc and C60 prepared by a vapor deposition method on an indium-tin oxide coated glass substrate. The OPD showed an incident photon-current efficiency (IPCE) of approximately 19% for light at a wavelength of 585 nm. This relatively high IPCE at 585 nm makes it suitable for detecting the fluorescence of resorufin (λem=585 nm), the product of the competitive ELISA, produced through the enzymatic reaction of Amplex Red with horseradish peroxidase (HRP) and H2O2. A fluorometric detector was assembled on a microchip by combining the fabricated OPD and a commercial LED as a photodetector and a light source, respectively. The photocurrent of the OPD due to the fluorescence of resorufin was proportional to the concentration of resorufin in the concentration range from 0 to 8 μM. When the fabricated OPD was used as a portable photodetector, the competitive ELISA of APnEOs using HRP labeled APnEOs (HRP-APnEOs) was performed on magnetic microbeads on which surface an anti-APnEOs antibody had been immobilized. A typical sigmoidal calibration curve was obtained and the data were in good agreement with a numerical simulation, where the photocurrent of the OPD was plotted against the concentration of APnEOs, determined via the competitive ELISA. The detection limit of the immunoassay for APnEOs was approximately 2 and 4 ppb in batch and flow system, respectively.

  17. Epigenetic Modulation in Periodontitis: Interaction of Adiponectin and JMJD3-IRF4 Axis in Macrophages.

    Science.gov (United States)

    Xuan, Dongying; Han, Qianqian; Tu, Qisheng; Zhang, Lan; Yu, Liming; Murry, Dana; Tu, Tianchi; Tang, Yin; Lian, Jane B; Stein, Gary S; Valverde, Paloma; Zhang, Jincai; Chen, Jake

    2016-05-01

    Emerging evidence suggests an important role for epigenetic mechanisms in modulating signals during macrophage polarization and inflammation. JMJD3, a JmjC family histone demethylase necessary for M2 polarization is also required for effective induction of multiple M1 genes by lipopolysaccharide (LPS). However, the effects of JMJD3 to inflammation in the context of obesity remains unknown. To address this deficiency, we firstly examined the expression of JMJD3 in macrophage isolated from bone marrow and adipose tissue of diet induced obesity (DIO) mice. The results indicated that JMJD3 was down-regulated in obesity. Adiponectin (APN), a factor secreted by adipose tissue which is down-regulated in obesity, functions to switch macrophage polarization from M1 to M2, thereby attenuating chronic inflammation. Intriguingly, our results indicated that APN contributed to JMJD3 up-regulation, reduced macrophage infiltration in obese adipose tissue, and abolished the up-regulation of JMJD3 in peritoneal macrophages isolated from DIO mice when challenged with Porphyromonas gingivalis LPS (pg.lps). To elucidate the interaction of APN and JMJD3 involved in macrophage transformation in the context of inflammation, we designed the loss and gain-function experiments of APN in vivo with APN(-/-) mice with experimental periodontitis and in vitro with macrophage isolated from APN(-/-) mice. For the first time, we found that APN can help to reduce periodontitis-related bone loss, modulate JMJD3 and IRF4 expression, and macrophage infiltration. Therefore, it can be inferred that APN may contribute to anti-inflammation macrophage polarization by regulating JMJD3 expression, which provides a basis for macrophage-centered epigenetic therapeutic strategies.

  18. Adiponectin attenuates lung fibroblasts activation and pulmonary fibrosis induced by paraquat.

    Science.gov (United States)

    Yao, Rong; Cao, Yu; He, Ya-rong; Lau, Wayne Bond; Zeng, Zhi; Liang, Zong-an

    2015-01-01

    Pulmonary fibrosis is one of the most common complications of paraquat (PQ) poisoning, which demands for more effective therapies. Accumulating evidence suggests adiponectin (APN) may be a promising therapy against fibrotic diseases. In the current study, we determine whether the exogenous globular APN isoform protects against pulmonary fibrosis in PQ-treated mice and human lung fibroblasts, and dissect the responsible underlying mechanisms. BALB/C mice were divided into control group, PQ group, PQ + low-dose APN group, and PQ + high-dose APN group. Mice were sacrificed 3, 7, 14, and 21 days after PQ treatment. We compared pulmonary histopathological changes among different groups on the basis of fibrosis scores, TGF-β1, CTGF and α-SMA pulmonary content via Western blot and real-time quantitative fluorescence-PCR (RT-PCR). Blood levels of MMP-9 and TIMP-1 were determined by ELISA. Human lung fibroblasts WI-38 were divided into control group, PQ group, APN group, and APN receptor (AdipoR) 1 small-interfering RNA (siRNA) group. Fibroblasts were collected 24, 48, and 72 hours after PQ exposure for assay. Cell viability and apoptosis were determined via Kit-8 (CCK-8) and fluorescein Annexin V-FITC/PI double labeling. The protein and mRNA expression level of collagen type III, AdipoR1, and AdipoR2 were measured by Western blot and RT-PCR. APN treatment significantly decreased the lung fibrosis scores, protein and mRNA expression of pulmonary TGF-β1, CTGF and α-SMA content, and blood MMP-9 and TIMP-1 in a dose-dependent manner (ppulmonary fibrosis in a dose-dependent manner, via suppression of lung fibroblast activation. Functional AdipoR1 are expressed by human WI-38 lung fibroblasts, suggesting potential future clinical applicability of APN against pulmonary fibrosis.

  19. Increased Hs-CRP/adiponectin ratio is associated with increase carotid intima-media thickness

    OpenAIRE

    Liao, Huocheng; Li, Zhiming; Zheng, Dongdan; Liu, Jianping; Liu, Yan; Xiao, Chun; Wang, Hongguang

    2014-01-01

    Background High sensitivity C-reactive protein (Hs-CRP) and adiponectin (APN) are two critical cytokines and exert inverse effects on atherosclerosis initiation and progression. The purpose of our study was to investigate the value of Hs-CRP and ANP ratio (Hs-CRP/APN ratio) on evaluating atherosclerosis progression. Method One hundred sixty consecutive participants underwent carotid intima-media thickness (CIMT) measured by ultrasound were enrolled and drawn fasting blood samples for plasma l...

  20. Identification of Hypertension Management-related Errors in a Personal Digital Assistant-based Clinical Log for Nurses in Advanced Practice Nurse Training

    Directory of Open Access Journals (Sweden)

    Nam-Ju Lee, DNSc, RN

    2010-03-01

    Conclusion: The Hypertension Diagnosis and Management Error Taxonomy was useful for identifying errors based on documentation in a clinical log. The results provide an initial understanding of the nature of errors associated with hypertension diagnosis and management of nurses in APN training. The information gained from this study can contribute to educational interventions that promote APN competencies in identification and management of hypertension as well as overall patient safety and informatics competencies.

  1. New targets to alleviate skeletal muscle inflammation: role of microRNAs regulated by adiponectin.

    Science.gov (United States)

    Boursereau, Raphaël; Abou-Samra, Michel; Lecompte, Sophie; Noel, Laurence; Brichard, Sonia M

    2017-02-27

    Muscle inflammation worsens metabolic disorders as well as devastating myopathies. The hormone adiponectin (ApN) has emerged has a master regulator of inflammation/immunity in several tissues including the skeletal muscle. In this work, we explore whether microRNAs regulated by ApN may represent novel mechanisms for controlling muscle inflammation. By screening arrays, we found miR-711 as a strong candidate for mediating ApN action. Thus, ApN-knockout mice showed decreased muscular expression of miR-711 together with enhanced inflammation/oxidative stress markers, while mice overexpressing ApN showed increased miR-711 levels. Likewise, electrotransfer of the ApN gene in muscle of ApN-knockout mice upregulated miR-711 while reducing inflammation and oxidative stress. Similar data were obtained in murine C2C12 cells or in human primary myotubes treated with ApN. MiR-711 overexpression downregulated several components of the Toll-like receptor-4 (TLR4) pathway, which led to repression of NF-κB activity and downstream pro-inflammatory cytokines. MiR-711 blockade had opposite effects. Moreover, muscle electrotransfer of pre-miR-711 recapitulated in vivo the anti-inflammatory effects observed in vitro. Thus, miR-711, which is upregulated by ApN represses TLR4 signaling, acting therefore as a major mediator of the anti-inflammatory action of ApN. This novel miRNA and its related target genes may open new therapeutic perspectives for controlling muscle inflammation.

  2. Adiponectin expression in patients with inflammatory cardiomyopathy indicates favourable outcome and inflammation control.

    Science.gov (United States)

    Bobbert, Peter; Scheibenbogen, Carmen; Jenke, Alexander; Kania, Gabriele; Wilk, Sabrina; Krohn, Stefanie; Stehr, Jenny; Kuehl, Uwe; Rauch, Ursula; Eriksson, Urs; Schultheiss, Heinz Peter; Poller, Wolfgang; Skurk, Carsten

    2011-05-01

    Circulating adiponectin (APN) is an immunomodulatory, pro-angiogenic, and anti-apoptotic adipocytokine protecting against acute viral heart disease and preventing pathological remodelling after cardiac injury. The purpose of this study was to describe the regulation and effects of APN in patients with inflammatory cardiomyopathy (DCMi). Adiponectin expression and outcome were assessed in 173 patients with DCMi, 30 patients with non-inflammatory DCM, and 30 controls. Mechanistic background of these findings was addressed in murine experimental autoimmune myocarditis (EAM), a model of human DCMi, and further elucidated in vitro. Adiponectin plasma concentrations were significantly higher in DCMi compared with DCM or controls, i.e. 6.8 ± 3.9 µg/mL vs. 5.4 ± 3.6 vs. 4.76 ± 2.5 µg/mL (P< 0.05, respectively) and correlated significantly with cardiac mononuclear infiltrates (CD3+: r(2)= 0.025, P= 0.038; CD45R0+: r(2)= 0.058, P= 0.018). At follow-up, DCMi patients with high APN levels showed significantly increased left ventricular ejection fraction improvement, decreased left ventricular end-diastolic diameter, and reduced cardiac inflammatory infiltrates compared with patients with low APN levels. A multivariate linear regression analysis implicated APN as an independent prognostic factor for inhibition of cardiac inflammation. In accordance with these findings in human DCMi, EAM mice exhibited elevated plasma APN. Adiponectin gene transfer led to significant downregulation of key inflammatory mediators promoting disease. Mechanistically, APN acted as a negative regulator of T cells by reducing antigen specific expansion (P< 0.01) and suppressed TNFα-mediated NFκB activation (P< 0.01) as well as release of reactive oxygen species in cardiomyocytes. Our results implicate that APN acts as endogenously upregulated anti-inflammatory cytokine confining cardiac inflammation and progression in DCMi.

  3. Closing the peroxy acetyl (PA radical budget: observations of acyl peroxy nitrates (PAN, PPN, and MPAN during BEARPEX 2007

    Directory of Open Access Journals (Sweden)

    B. W. LaFranchi

    2009-04-01

    Full Text Available Acyl peroxy nitrates (APNs, also known as PANs are formed from the oxidation of aldehydes and other oxygenated VOC (oVOC in the presence of NO2. Formation of APNs suppresses NOx (NOx≡NO+NO2 in urban areas and enhances NOx downwind in urban plumes, increasing the rate of ozone production throughout an urban plume. APNs also redistribute NOx on global scales, enhancing NOx and thus ozone production. There are both anthropogenic and biogenic oVOC precursors to APNs, but a detailed evaluation of their chemistry against observations has proven elusive. Here we describe measurements of PAN, PPN, and MPAN along with the majority of chemicals that participate in their production and loss, including OH, HO2, numerous oVOC, and NO2. Observations were made during the Biosphere Effects on AeRosols and Photochemistry Experiment (BEARPEX 2007 in the outflow of the Sacramento urban plume. These observations are used to evaluate a detailed chemical model of APN ratios and concentrations. We find the ratios of APNs are nearly independent of the loss mechanisms and thus an especially good test of our understanding of their sources. We show that oxidation of methylvinyl ketone, methacrolein, methyl glyoxal, biacetyl and acetaldehyde are all significant sources of the PAN+peroxy acetyl (PA radical reservoir, with methylvinyl ketone (MVK often being the primary non-acetaldehyde source. At high temperatures, oxidation of non-acetaldehyde PA radical sources contributes over 60% to the total PA production rate. An analysis of absolute APN concentrations reveals a missing APN sink that can be resolved by increasing the PA+∑RO2 rate constant by a factor of 3.

  4. Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

    Energy Technology Data Exchange (ETDEWEB)

    Cong, Yingying; Li, Xiaoxue; Bai, Yunyun [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); Lv, Xiaonan [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience & Technology of China, Beijing 100090 (China); Herrler, Georg [Institute for Virology, University of Veterinary Medicine, Hannover D-30559 (Germany); Enjuanes, Luis [Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid (Spain); Zhou, Xingdong [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); Qu, Bo [Faculty of Life Sciences, Northeast Agricultural University, Harbin 150030 (China); Meng, Fandan [Institute for Virology, University of Veterinary Medicine, Hannover D-30559 (Germany); Cong, Chengcheng [College Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161 (China); Ren, Xiaofeng; Li, Guangxing [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China)

    2015-04-15

    Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells. - Highlights: • PEDV infection of polarized intestinal epithelial cells (IECs) was characterized. • Porcine aminpeptidase N (pAPN) facilitated PEDV infection in IECs. • PEDV entry into and release from polarized cell via its apical membrane. • PEDV infection may proceed by lateral spread of virus in IECs.

  5. Evolvement of French advanced practice nurses.

    Science.gov (United States)

    Bonnel, Galadriel

    2014-04-01

    The purpose of this review is to chronicle the development of the advanced practice nurse (APN) in France and compare international APN indictors of quality care with French studies. A review of the literature was performed by accessing the MEDLINE, Science Direct, and Cochrane Databases for studies of quality of care by APNs during 1965-2012. The author's participation on a national task force in collaboration with the French Ministry of Health provided additional information. After applying limits of this search, 36 studies fulfilled inclusion and exclusion criteria. In both the French and international APN nursing literature, the most frequently described quality of care measures were level of patient satisfaction and other patient outcomes (clinical and laboratory measures) according to evidence-based guidelines. In three French studies (nephrology, neuro-oncology, and urology settings), nurses performed direct patient care and were legally permitted to take on some limited responsibilities usually held by French physicians, including clinical examinations, diagnosing, and prescribing. Creation of the APN role in France can respond to public health challenges including the rising incidence of chronic diseases and an impending physician shortage. Future APN research should focus on rigorous, innovative design development including collaborative care models. ©2013 The Author(s) ©2013 American Association of Nurse Practitioners.

  6. RNAi in the striped stem borer, Chilo suppressalis, establishes a functional role for aminopeptidase N in Cry1Ab intoxication.

    Science.gov (United States)

    Wang, X Y; Du, L X; Liu, C X; Gong, L; Han, L Z; Peng, Y F

    2017-02-01

    The striped stem borer, Chilo suppressalis, is a major target pest of transgenic rice expressing the Cry1Ab protein from the bacterium Bacillus thuringiensis (Bt) in China. Evolution of resistance in this pest is a major threat to the durability of Bt rice. Since Bt exerts its activity through binding to specific receptors in the midgut of target insects, identification of functional Cry1Ab receptors in the midgut of C. suppressalis larvae is crucial to evaluate potential resistance mechanisms and develop effective strategies for delaying insect resistance. In this work, we identified the putative Cry1Ab toxin-binding protein, aminopeptidase-N (APN), in the midgut of C. suppressalis by ligand blot and mass spectrometry. After cloning the full-length cDNAs encoding APN isoforms from the C. suppressalis larval midgut, we studied their spatiotemporal expression in different gut tissues and developmental stages. Furthermore, RNA interference (RNAi) against C. suppressalis aminopeptidases (CsAPNs) was employed to illustrate a functional role for CsAPNs in Cry1Ab toxicity to C. suppressalis larvae using injection and oral delivery of Stealth™ siRNA. Down-regulating the expression of CsAPNs by RNAi was closely associated with reduced susceptibility of C. suppressalis to Cry1Ab. These data provide the first direct evidence that CsAPNs participate in the mode of Cry1Ab action and may act as the functional receptor of Cry1A in C. suppressalis larvae.

  7. The role of adiponectin in human vascular physiology.

    Science.gov (United States)

    Vaiopoulos, Aristeidis G; Marinou, Kyriakoula; Christodoulides, Constantinos; Koutsilieris, Michael

    2012-03-08

    Adiponectin (ApN) is an adipose tissue-derived hormone which is involved in a wide variety of physiological processes including energy metabolism, inflammation, and vascular physiology via actions on a broad spectrum of target organs including liver, skeletal muscle, and vascular endothelium. Besides possessing insulin sensitizing and anti-inflammatory properties ApN also exerts a pivotal role in vascular protection through activation of multiple intracellular signaling cascades. Enhancement of nitric oxide generation and attenuation of reactive oxygen species production in endothelial cells along with reduced vascular smooth muscle cell proliferation and migration constitute some of ApN's vasoprotective actions. Additionally, recent data indicate that ApN has direct myocardio-protective effects. Decreased plasma ApN levels are implicated in the pathogenesis of the metabolic syndrome and atherosclerosis and may serve as a diagnostic and prognostic biomarker as well as a rational pharmaco-therapeutic target to treat these disorders. This review article summarizes recent work on the cardiovascular actions of ApN.

  8. Advanced practice nursing, health care teams, and perceptions of team effectiveness.

    Science.gov (United States)

    Kilpatrick, Kelley; Lavoie-Tremblay, Mélanie; Ritchie, Judith A; Lamothe, Lise

    2014-01-01

    This article summarizes the results of an extensive review of the organizational and health care literature of advanced practice nursing (APN) roles, health care teams, and perceptions of team effectiveness. Teams have a long history in health care. Managers play an important role in mobilizing resources, guiding expectations of APN roles in teams and within organizations, and facilitating team process. Researchers have identified a number of advantages to the addition of APN roles in health care teams. The process within health care teams are dynamic and responsive to their surrounding environment. It appears that teams and perceptions of team effectiveness need to be understood in the broader context in which the teams are situated. Key team process are identified for team members to perceive their team as effective. The concepts of teamwork, perceptions of team effectiveness, and the introduction of APN roles in teams have been studied disparately. An exploration of the links between these concepts may further our understanding the health care team's perceptions of team effectiveness when APN roles are introduced. Such knowledge could contribute to the effective deployment of APN roles in health care teams and improve the delivery of health care services to patients and families.

  9. A conceptual framework for advanced practice nursing in a pediatric tertiary care setting: the SickKids' experience.

    Science.gov (United States)

    LeGrow, Karen; Hubley, Pam; McAllister, Mary

    2010-05-01

    Advanced practice nurses (APNs) at The Hospital for Sick Children (SickKids) are pediatric healthcare providers who integrate principles and theories of advanced nursing with specialty knowledge to provide autonomous, independent, accountable, ethical and developmentally appropriate care in complex, often ambiguous and rapidly changing healthcare environments. Caring for children and adolescents requires culturally sensitive and family-centred approaches to care that incorporate a unique body of knowledge. Family-centred care is an approach to planning, delivery and evaluation of healthcare that is governed by the establishment of mutually beneficial partnerships among APNs, health professionals and children/families. The cornerstone of APN practice at SickKids is the recognition of "family" as the recipients of care. By valuing and developing relationships with families, APNs promote excellence in healthcare across the care continuum to optimize the child's and family's physical, emotional, social, psychological and spiritual well-being. This paper outlines the evolution of advanced practice nursing at SickKids, beginning with the introduction of APN roles in the 1970s and culminating in the current critical mass of APNs who have been integrated throughout the hospital's infrastructure. We describe the process used to create a common vision and a framework to guide pediatric advanced nursing practice.

  10. Fabrication of Apigenin loaded gellan gum-chitosan hydrogels (GGCH-HGs) for effective diabetic wound healing.

    Science.gov (United States)

    Shukla, Rajesh; Kashaw, Sushil K; Jain, Alok Pal; Lodhi, Santram

    2016-10-01

    The Apigenin (APN) was isolated from ethanolic extract of M. alba leaves and screened by in-vivo wound models (Diabetic and Dead space) in rats. Apigenin loaded hydrogel (HGs) was prepared using gellan gum-chitosan (GGCH) with PEG as a cross linker and characterized for various parameter like AFM, swelling property, entrapment efficiency and drug release. Further performance of hydrogel was evaluated by wound healing activity tested against wound contraction, collagen content, dried granuloma weights and antioxidant activity. The percent entrapment efficiency of optimized hydrogel found to be 87.15±1.20. APN loaded GGCH-HGs were able to release 96.11% APN in 24h. The level of superoxide dismutase (SOD) and catalase were found increased significantly in granuloma tissue of APN treated group. APN GGCH-HGs found higher wound healing effect in diabetic as well as normal wound tissues with significant antioxidant activity. Results proven the utility of prepared hydrogel (APN loaded GGCH-HGs) seems to be highly suitable for wound healing due to its unique properties of biocompatibility, biodegradability, moist nature and antioxidant effectiveness.

  11. Mechanisms for the inversion of chirality: Global reaction route mapping of stereochemical pathways in a probable chiral extraterrestrial molecule, 2-aminopropionitrile

    Energy Technology Data Exchange (ETDEWEB)

    Kaur, Ramanpreet; Vikas, E-mail: qlabspu@pu.ac.in, E-mail: qlabspu@yahoo.com [Quantum Chemistry Group, Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160014 (India)

    2015-02-21

    2-Aminopropionitrile (APN), a probable candidate as a chiral astrophysical molecule, is a precursor to amino-acid alanine. Stereochemical pathways in 2-APN are explored using Global Reaction Route Mapping (GRRM) method employing high-level quantum-mechanical computations. Besides predicting the conventional mechanism for chiral inversion that proceeds through an achiral intermediate, a counterintuitive flipping mechanism is revealed for 2-APN through chiral intermediates explored using the GRRM. The feasibility of the proposed stereochemical pathways, in terms of the Gibbs free-energy change, is analyzed at the temperature conditions akin to the interstellar medium. Notably, the stereoinversion in 2-APN is observed to be more feasible than the dissociation of 2-APN and intermediates involved along the stereochemical pathways, and the flipping barrier is observed to be as low as 3.68 kJ/mol along one of the pathways. The pathways proposed for the inversion of chirality in 2-APN may provide significant insight into the extraterrestrial origin of life.

  12. Childhood acute pyelonephritis: comparison of power Doppler sonography and Tc-DMSA scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Stogianni, Aggeliki; Oikonomou, Ippoliti; Dimitriadis, Athanasios [Aristotle University of Thessaloniki, Department of Radiology, AHEPA Hospital, Thessaloniki (Greece); Nikolopoulos, Panagiotis [424 Army Hospital, Department of Radiology, Thessaloniki (Greece); Gatzola, Magdalini [Aristotle University of Thessaloniki, 2nd Paediatric Clinic, AHEPA Hospital, Thessaloniki (Greece); Balaris, Vassilios [Aristotle University of Thessaloniki, Department of Nuclear Medicine, AHEPA Hospital, Thessaloniki (Greece); Farmakiotis, Dimitrios [Infectious Diseases Hospital of Thessaloniki, Department of Medicine, Thessaloniki (Greece)

    2007-07-15

    Tc 99m DMSA scintigraphy is regarded as the gold standard for the detection and localization of acute pyelonephritis (APN) in children. Power Doppler sonography (PD US) is a radiation-free and cost-effective technique that could be useful in the diagnosis of APN in children. To compare the predictive value of PD US with DMSA scintigraphy in the diagnosis of APN in children. A total of 74 neonates and children with clinical findings consistent with possible upper urinary tract infection were evaluated with PD US and DMSA scintigraphy. Children with anatomic (grey-scale) abnormalities were excluded. A total of 147 kidneys were examined within the first 48 h after the onset of symptoms. Each kidney was divided into three zones (upper, middle, and lower third). APN was diagnosed by PD US in 46 kidneys. Sensitivity and specificity for detecting APN using DMSA scintigraphy as the reference standard were 73.8% and 85.7%, respectively. There was good agreement between PD US and DMSA scintigraphy in the localization of lesions. In clinically suspected APN, PD US has acceptable specificity and sensitivity, if performed within the first 48 h and could be helpful in neonates and children under 3 months of age in whom the use of scintigraphy is generally discouraged. (orig.)

  13. Pengembangan Peta Interaktif Tiga Dimensi Gedung Rektorat Institut Teknologi Sepuluh Nopember Menggunakan Unity3d Engine

    Directory of Open Access Journals (Sweden)

    Muhammad Imam Ghazali

    2015-03-01

    Full Text Available Penggunaan teknologi informasi dalam pemetaan digital sudah lama digunakan. Dahulu orang cenderung melakukan pemetaan digital secara 2D untuk memetakan suatu area atau gedung, namun pemetaan secara 2D sendiri tidak memberikan informasi secara mendetail tentang keadaan dan topografi dari area atau gedung tersebut. Seiring dengan perkembangan teknologi informasi penggunaan teknologi 3D pun mulai digunakan dalam pemetaan secara digital agar dapat memberikan informasi yang lebih mendetail tentang keadaan suatu area atau gedung. Untuk membangun sebuah peta 3D dapat dilakukan menggunakan engine 3D yang biasanya digunakan untuk membuat game. Dengan memanfaatkan Unity3D Engine penulis akan membangun sebuah pemetaan digital secara 3D agar dari pemetaan tersebut dapat memberikan informasi yang akurat dan tepat mengenai keadaan area yang dipetakan sesuai dengan kenyataan. Dalam pembuatan akan dipetakan gedung Rektorat Institut Teknologi Sepuluh Nopember Surabaya. Pemetaan secara 3D ini dapat memberikan informasi yang akurat dan mendetail kepada pengguna peta 3D ini nantinya tentang keadaan dari gedung Rektorat.

  14. PENGARUH PERBANDINGAN AIR DAN BUBUK TERHADAP LAMANYA WAKTU SETTING DENTAL STONE

    OpenAIRE

    Sila, Patri

    2012-01-01

    2012 Patri Sila. Dental stone adalah bahan yang terbuat dari gipsum yang kristalisasinya dikontrol secara hati ??? hati dengan tekanan uap dalam wadah tertutup dan biasanya di sebut sebagai ??-hemihidrat. Secara komersial, dental stone dibuat dari gipsum yang dipanaskan pada suhu 125?? C dibawah tekanan uap dalam autoklaf secara terus menerus sehingga terbentuk hemihidrat dengan porositas yang rendah. Tujuan penelitian ini adalah untuk mengetahui pengaruh perbandingan air dan bubuk terh...

  15. Strategi Banquet Departemen Dalam Meningkatkan Pendapatan Di JW Marriot Hotel Medan

    OpenAIRE

    Dede Anwar Putra Sinaga

    2009-01-01

    Saat dikemukakannya visit Indonesia 2009 oleh mentri kepariwisataan Indonesia tahun lalu, muncul pula investor-investor asing yang secara bersamaan menanamkan sahamnya di medan untuk membangun hotel-hotel berbintang lima (*****) yang berkelas internasional. Menariknya lagi pembangunan hingga selesainya bangunan hotel-hotel yang megah dan mewah dijantung kota medan ini adalah secara bersamaan. Persaingan yang tercipta dikarenakan munculnya hotel berkelas internasional secara bersamaan ini...

  16. ECOLOGY AND INFECTION RATES OF NATURAL VECTORS OF FILARIASIS IN TANAH INTAN, SOUTH KALIMANTAN (BORNEO), INDONESIA

    OpenAIRE

    Soeroto Atmosoedjono; Purnomo Purnomo; Sutanti Ratiwayanto; Harijani A. Marwoto; Bangs, Michael J.

    2012-01-01

    Data ekologi nyamuk vektor dan tingkat infeksi filaría secara alami dan secara buatan telah diperoleh dari perkebunan karet di Kalimantan Selatan, Indonesia. Berbagai macam cara penangkapan dalam kondisi ekologi yang berbeda telah dipakai dalam pengumpulan 51 jenis nyamuk (N = 95.735). Pembedahan nyamuk, infeksi buatan dan identifikasi larva filaría mengikuti prosedur dan kunci yang sudah baku. Infeksi filaría Brugia, Breinlia dan Cardiofílaria secara alami ditemukan pada nyamuk Coquillettidi...

  17. Acute pyelonephritis in transplanted kidneys: can diffusion-weighted magnetic resonance imaging be useful for diagnosis and follow-up?

    Science.gov (United States)

    Faletti, Riccardo; Cassinis, Maria Carla; Gatti, Marco; Giglio, Jacopo; Guarnaccia, Carla; Messina, Marina; Bergamasco, Laura; Fonio, Paolo

    2016-03-01

    To assess reliability of diffusion-weighted magnetic resonance imaging (DW-MRI) in the management of acute pyelonephritis (APN) foci in transplanted kidneys. In the 2012-2014 period, 24 kidney-transplanted patients underwent MR screening for clinical suspicion of APN. Two readers independently analyzed all images, establishing presence and location of APN foci. The 22 patients who were positive at the MR exam constituted the study population. For each patient the apparent diffusion coefficient (ADC) was measured in the APN foci and in three sites of the healthy parenchyma (case-control comparison). The data were matched to the laboratory measurements for white blood cell, C-reactive protein, and serum creatinine. Forty-six APN foci were found in 22/24 patients. At the acute stage, the difference in ADC between healthy parenchyma and APN foci was significant (2.06 ± 0.16 vs. 1.43 ± 0.32 × 10(-3) mm(2)/s; p < 0.0001). The performance of ADC as APN indicator was tested by the receiving operating characteristics (ROC) curve: the area under curve AUC = 0.99 witnessed an excellent discriminatory ability, with threshold APN/normal parenchyma 1.9 × 10(-3) mm(2)/s. At the 1-month follow-up 43/46 APN foci were no longer visible, with ADC values significantly higher than at the acute stage; all laboratory data were physiological, with WBC significantly reduced from the acute phase (5.2 ± 1.6 × 10(9)/L vs. 10.6 ± 4.8 × 10(9)/L; p < 0.0001). The other 3 patients underwent further therapy and exams, including a third MR. DW-MRI with ADC measurement seems to be a reliable tool in diagnosing and monitoring APN foci in transplanted kidneys, with clinical impact on patient management.

  18. PENGARUH JUS DAUN SIRIH (Piper betle Linn SEBAGAI BAHAN PRECURING DAN LAMA PENYIMPANAN TERHADAP KOMPOSISI KIMIA DAN ANGKA PEROKSIDA DENDENG AYAM PETELUR

    Directory of Open Access Journals (Sweden)

    Aloysia Tenny Damayanti Indriastuti

    2012-09-01

    mempertahankan komposisi kimia secara proposional dan menekan angka peroksida sehingga memperpanjang lama penyimpanan dendeng ayam petelur afkir. (Kata kunci: Daging ayam, Daun sirih, Dendeng, Komposisi kimia, Angka peroksida

  19. KONSEP RUANG TERBUKA PUBLIK MAHASISWA SEBAGAI PENGHUBUNG ANTAR UNIT DI UNIVERSITAS TANJUNGPURA

    Directory of Open Access Journals (Sweden)

    Yudi Purnomo

    2014-01-01

    Zahnd, Markus. 2009.  Pendekatan dalam Perancangan Arsitektur: Metode untuk Menganalisis dan Merancang Arsitektur secara Efektif.Penerbit Kanisius, Semarang: Soegijapranata University Press, Yogyakarta

  20. Variation of maternal milk adiponectin and its correlation with infant growth%母乳脂联素浓度变化与婴儿生长发育的相关性研究

    Institute of Scientific and Technical Information of China (English)

    王艳艳; 张卓君; 姚文; Ardythe Morrow; 彭咏梅

    2011-01-01

    Objective To investigate the variation of human breast milk adiponectin (APN)concentration during lactation, analyze the relationship of APN concentrations in human breast milk with APN in infant serum, determine the association between maternal milk APN and infant body proportionality in the first year of life, and the period of greatest human milk exposure.Method Subjects included 73mother-infant pairs from Shanghai International Peace Maternity & Child Health Hospital.Maternal milk was collected at 2, 4, 13, 26 weeks and infant serum was collected at 26 weeks.The concentration of APN in maternal milk and infant serum were measured with commercially available ELISAs (R&D Systems).Weight-for-age Z-scores (WAZ) , length-for-age Z-scores ( LAZ ) , weight-for-length Z-scores ( WLZ ) and body mass index Z-scores ( zBMI ) of infants up to 1 year of age were calculated using World Health Organization (WHO) standards.Then the variation of maternal milk APN was investigated, the correlation between maternal milk APN and infant serum APN was analyzed; multivariate regression analysis was used to analyze covariates correlated with infant body proportionality, meanwhile determined the association between maternal milk APN and infant anthropometrics.Result The concentration of APN in maternal milk at 2, 4,13, 26 weeks was respectively (14.62 μg/L, 5.93-140.40 μg/L), (7.32 μg/L, 2.04-29.35 μg/L),(6.84 μg/L, 2.72-15.65 μg/L) and (4.88 μg/L, 1.12-13.38 μg/L).Higher milk APN in postpartum 2weeks was associated with lower infant WAZ at 13, 26 and 52 weeks ( P < 0.05 ).Milk APN in postpartum 4 weeks was inversely related to infant WAZ, WLZ, zBMI at 13, 26 and 52 weeks ( all P < 0.05 ).Meanwhile, milk APN in postpartum 13 weeks had significantly negative correlation with infant WAZ, WLZ,zBMI at 13, 26 and 52 weeks (P<0.01).Milk APN in postpartum 26 weeks also was inversely associated with infant WAZ, WLZ and zBMI at 52 weeks (P <0.05).After adjusting covariates like

  1. In vivo susceptibility of ESBL producing Escherichia coli to ceftriaxone in children with acute pyelonephritis

    Directory of Open Access Journals (Sweden)

    Peco-Antić Amira

    2012-01-01

    Full Text Available Introduction. The choice of empiric therapy of acute pyelonephritis (APN in children should be based on the knowledge of Escherichia coli (E. coli as the most common uropathogen and its antibiotic sensitivities considering that nowadays ESBL-producing [ESBL (+] E. coli is on the rise worldwide. Objective. To examine in vivo susceptibility of ESBL (+ E. coli to ceftriaxone (CTX, and to evaluate the options for empiric therapy for APN in children. Methods. Retrospective study of CTX empiric therapy of APN in children treated at the University Children΄s Hospital in Belgrade from January 2005 to December 2009. ESBL phenotypic confirmatory test with ceftazidime, CTX and cefotaxime was performed for all urine isolates by disc diffusion method on Mueller-Hinton agar plates. In vivo sensitivity of CTX documented by clinical response to empiric CTX therapy was compared between two groups of children: group I with ESBL (+ E. coli and group II with ESBL (- E. coli APN. Results. Group I with ESBL (+ APN consisted of 94 patients and group II of 120 patients with ESBL (- APN, respectively. All patients received CTX as empiric therapy at a mean dose of 66.9 mg during 7.2±2.6 days of therapy. Clinical effect of CTX was similar in patients with ESBL (+ compared to those with ESBL (- APN. Conclusions. In vitro resistance of ESBL E. coli to CTX determined by standard methods is not sufficiently predictive for its in vivo sensitivity. Therefore CTX may be used as empiric therapy for acute pyelonephritis in children.

  2. Circulating adiponectin levels and risk of endometrial cancer: Systematic review and meta-analysis.

    Science.gov (United States)

    Li, Zhi-Jun; Yang, Xue-Ling; Yao, Yan; Han, Wei-Qing; Li, B O

    2016-06-01

    Previous epidemiological studies have presented conflicting results regarding associations between circulating adiponectin (APN) levels and the risk of endometrial cancer. Thus a meta-analysis was performed to investigate the association between these factors. Multiple electronic sources, including PubMed, SpringerLink and Google Scholar databases were searched to identify relevant studies for the present meta-analysis. All of the selected studies examined the correlation between circulating APN levels and endometrial cancer. The standardized mean difference (SMD) and 95% confidence intervals (CIs) were estimated and pooled using meta-analysis methods. Overall, 18 case-control studies met the inclusion criteria. A total of 5,692 participants and 2,337 cases of endometrial cancer were included in this meta-analysis. The SMD of the pooled analysis (95% CI) were -1.96 (-2.60, -1.31), P=0.000. When the cancer grades were compared, the APN values were not significantly different between the grades of endometrial cancer [G1 vs. G3, 1.02 (-0.68, 2.72), P>0.05; G1 vs. G2, 0.34 (-0.86, 1.54), P>0.05]. However, there was a significant association between high APN levels and postmenopausal endometrial cancer cases with an SMD (95% CI) of -2.27 (-4.36, -0.18) and P0.05. The low circulating APN level increases the risk of endometrial cancer, whereas the high APN level decreases this risk in postmenopausal women. Circulating APN as simple biomarkers may be a promising tool for the prevention, early diagnosis and disease monitoring of endometrial cancer.

  3. 20 Years of Developing Capacity for Action-Oriented Collaborative Regional Research in the Asia-Pacific Region

    Science.gov (United States)

    Tupas, L. M.; Stevenson, L. A.

    2015-12-01

    During its 3rd strategic phase, which ran from 2010 to 2015, the APN provided support for 123 projects through its competitive collaborative regional research and capacity development programmes. With over 250 peer-reviewed papers and the underlying philosophy that the regional research it undertakes engages at least two developing countries, the 3rd strategic phase is not only improving the research capabilities of nations in the region, but is engaging the developing country community in underpinning policy-relevant research. The extent to which science is contributing to policy is further evident in that 69% of the activities conducted had some form of science-policy mechanism built in to the project activities. The period of the 3rd Strategic Phase has been witness to significant changes in the make-up of the "conventional" global change arena, with the transition of some global change programmes into the new "Future Earth" initiative. At the same time, major events such as the Rio+20 Summit, the post-2015 Sustainable Development Goals, the establishment of an IPCC-related platform for biodiversity, i.e. IPBES, and the evolving engagement of science and policy communities has kept the APN busy at what it does best - networking and partnering with the international community. The APN has embraced these changes through its dynamism, allowing the APN to meet not only the needs of the scientists and decision makers its serves in the region but those of the international science communities as well. The challenge for the APN in its next two decades will be to ensure alignment with the transforming global change arena. With key phrases such as "policy-relevant science" and "science-policy interactions" being adopted broadly by the community at large these days, the APN's niche in the global community has received considerable recognition, particularly as other institutions strive to adopt similar practices that the APN has built over the last 20 years.

  4. Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies.

    Directory of Open Access Journals (Sweden)

    Juan Reguera

    Full Text Available The coronaviruses (CoVs are enveloped viruses of animals and humans associated mostly with enteric and respiratory diseases, such as the severe acute respiratory syndrome and 10-20% of all common colds. A subset of CoVs uses the cell surface aminopeptidase N (APN, a membrane-bound metalloprotease, as a cell entry receptor. In these viruses, the envelope spike glycoprotein (S mediates the attachment of the virus particles to APN and subsequent cell entry, which can be blocked by neutralizing antibodies. Here we describe the crystal structures of the receptor-binding domains (RBDs of two closely related CoV strains, transmissible gastroenteritis virus (TGEV and porcine respiratory CoV (PRCV, in complex with their receptor, porcine APN (pAPN, or with a neutralizing antibody. The data provide detailed information on the architecture of the dimeric pAPN ectodomain and its interaction with the CoV S. We show that a protruding receptor-binding edge in the S determines virus-binding specificity for recessed glycan-containing surfaces in the membrane-distal region of the pAPN ectodomain. Comparison of the RBDs of TGEV and PRCV to those of other related CoVs, suggests that the conformation of the S receptor-binding region determines cell entry receptor specificity. Moreover, the receptor-binding edge is a major antigenic determinant in the TGEV envelope S that is targeted by neutralizing antibodies. Our results provide a compelling view on CoV cell entry and immune neutralization, and may aid the design of antivirals or CoV vaccines. APN is also considered a target for cancer therapy and its structure, reported here, could facilitate the development of anti-cancer drugs.

  5. The combination of urinary IL - 6 and renal biometry as useful diagnostic tools to differentiate acute pyelonephritis from lower urinary tract infection.

    Science.gov (United States)

    Azab, Sherif; Zakaria, Mostafa; Raafat, Mona; Seief, Hadeel

    2016-01-01

    To evaluate the role of renal ultrasound (RUS) and urinary IL-6 in the differentiation between acute pyelonephritis (APN) and lower urinary tract infection (LUTI). This prospective study was carried out at the Pediatric and urology outpatient and inpatient departments of Cairo University Children's Hospital as well as October 6 University Hospital and it included 155 children between one month and fourteen years old with positive culture UTI. Patients were categorized into APN and LUTI based on their clinical features and laboratory parameters. Thirty healthy children, age and sex matched constituted the control group. Children with positive urine cultures were treated with appropriate antibiotics. Before treatment, urinary IL-6 was measured by enzyme immunoassay technique (ELISA), and renal ultrasound (RUS) was done. CRP (C-reactive protein), IL-6 and RUS were repeated on the 14th day of antibiotic treatment to evaluate the changes in their levels in response to treatment. UIL-6 levels were more significantly higher in patients with APN than in patients with LUTI (24.3±19.3pg/mL for APN vs. 7.3±2.7pg/mL in LUTI (95% CI: 2.6-27.4; p20pg/mL and serum CRP >20μg/mL were highly reliable markers of APN. Mean renal volume and mean volume difference between the two kidneys in the APN group were more than that of the LUTI and control groups (Purinary IL-6 levels have a highly dependable role in the differentiation between APN and LUTI especially in places where other investigations are not available and/ or affordable. Copyright© by the International Brazilian Journal of Urology.

  6. Effects of gentamicin monotherapy for the initial treatment of community-onset complicated non-obstructive acute pyelonephritis due to Enterobacteriaceae in elderly and non-elderly women.

    Science.gov (United States)

    Wie, S-H; Kim, H W; Chang, U-I

    2014-11-01

    Aminoglycosides may serve as fluoroquinolone-sparing or cephalosporin-sparing agents if the clinical effectiveness of aminoglycoside monotherapy is demonstrated. The purposes of this study were to investigate the clinical efficacy of gentamicin as an initial empirical antimicrobial agent and to evaluate the effects of gentamicin resistance on clinical outcomes in women with complicated non-obstructive acute pyelonephritis (APN). Medical records of 1066 women with a diagnosis of APN were reviewed retrospectively. We enrolled 275 women with community-onset complicated non-obstructive APN due to Enterobacteriaceae who received gentamicin as their initial antibiotic. Of these 275 patients, 43 had gentamicin-resistant (GM-R) Enterobacteriaceae APN, and 232 had gentamicin-susceptible (GM-S) Enterobacteriaceae APN. The early clinical success rates were 67.4% (29/43) versus 89.7% (208/232) at 72 h in the GM-R versus the GM-S groups (p 0.001). The overall clinical cure rate was 100% (43/43) and 98.7% (229/232) in the GM-R and GM-S groups, respectively. The duration of hospital stay was significantly longer in the elderly, although there were no significant differences in the rates of early clinical success, final clinical cure, mortality, and time to fever clearance between the elderly and non-elderly groups. Resistance of Enterobacteriaceae to gentamicin, haematuria and serum C-reactive protein level≥20 mg/dL were independently associated with early clinical failure. Gentamicin can be an effective initial antibiotic option for empirical therapy in women with community-onset complicated APN who do not need urological interventional procedures. The use of gentamicin may contribute to a reduction of fluoroquinolone or broad-spectrum cephalosporin use in the treatment of complicated APN.

  7. Acute Pyelonephritis in Renal Allografts–A New Role for MicroRNAs?

    Science.gov (United States)

    Oghumu, Steve; Bracewell, Anna; Nori, Uday; Maclean, Kirsteen H.; Balada-Lasat, Joan-Miquel; Brodsky, Sergey; Pelletier, Ronald; Henry, Mitchell; Satoskar, Abhay R.; Nadasdy, Tibor; Satoskar, Anjali A.

    2014-01-01

    Background Acute pyelonephritis (APN) versus acute rejection (AR) is a frequently encountered diagnostic and therapeutic dilemma in kidney transplants. Variable culture results, overlapping histologic features, and persistent graft dysfunction despite antibiotics are frequently encountered. Therefore, we explored the utility of intragraft microRNA profiles to distinguish between allograft APN and AR. Materials and Methods Between 2003 and 2011, we identified 49 patients with biopsy features of APN, within the first 2 years posttransplant. MicroRNA profiling was performed on 20 biopsies (normal kidney, n=4; unequivocal AR, n=5; features of APN, n=11). Results Only 32% (16/49) of the patients had concomitant positive urine cultures at biopsy, and in 8 of 16 patients, colony count was less than 105 CFU/mL. In 14 of 49 patients, positive urine culture did not coincide with the biopsy, and in 19 of 49 patients, urine cultures were negative. On microRNA profiling, good clustering was seen among the normal kidneys and among AR biopsies. Among the 11 biopsies with features of APN, 4 biopsies showed good clustering with a pattern distinct from AR; (these patients recovered graft function with antibiotics); 7 of 11 biopsies showed heterogeneity in microRNA profiles and variable outcomes with antibiotic treatment. We identified a panel of 25 microRNAs showing statistical difference in expression between AR and APN. MiR-99b, miR-23b let-7b-5p, miR-30a, and miR-145 were validated using qPCR. Conclusion Allograft pyelonephritis can be a diagnostic and therapeutic challenge. A gestalt approach is required. In addition to histology and cultures, differential intragraft microRNA expression may prove helpful to distinguish APN from AR in renal allograft biopsies. PMID:24521778

  8. Are the unenhanced and excretory CT phases necessary for the evaluation of acute pyelonephritis?

    Science.gov (United States)

    Taniguchi, Lincoln S; Torres, Ulysses S; Souza, Saulo M; Torres, Lucas R; D'Ippolito, Giuseppe

    2017-05-01

    Background The most widely accepted computed tomography (CT) protocol for diagnosis of acute pyelonephritis (APN) includes at least a pre- and post-contrast scan, which may expose patients to higher doses of ionizing radiation. Purpose To establish the accuracy, reproducibility, and degree of confidence in CT diagnosis of acute pyelonephritis (APN) and urolithiasis using only images obtained during the nephrographic phase. Material and Methods A retrospective study of 100 consecutive patients (88 women; age range, 19-70 years) with clinical and laboratory suspicion of APN and who underwent triphasic abdominal CT scans (non-contrast, nephrographic, and excretory phases) was performed. Two readers first evaluated independently only the nephrographic phase of scans, and, in a second session, the entire study. The diagnostic reference standard was settled by a third experienced radiologist who reviewed all triphasic scans and clinical data. Results The accuracy of only nephrographic phase for diagnosis of APN and urolithiasis was in the range of 90.3-91.78% and 96.27-99.25%, respectively. There was no significant difference in comparison with the triphasic reading (z: -0.4 - 0.2; P = 0.34-0.83). The average degree of confidence for APN also showed no significant variation for both readers ( P = 0.4 and 0.08). Almost perfect inter-observer agreements for the diagnosis of APN (k = 0.86, P < 0.001) and for urolithiasis (k = 0.84, P < 0.001) were observed when considering only the nephrographic phase. Conclusion CT assessment of APN and urolithiasis can be accurately performed using only the late nephrographic phase, with consequent dose reduction.

  9. Adiponectin enhances osteogenic differentiation in human adipose-derived stem cells by activating the APPL1-AMPK signaling pathway

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Tong; Wu, Yu-wei; Lu, Hui; Guo, Yuan [Second Dental Center, Peking University School and Hospital of Stomatology, Beijing (China); National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing (China); Tang, Zhi-hui, E-mail: tang_zhihui@live.cn [Second Dental Center, Peking University School and Hospital of Stomatology, Beijing (China); National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing (China)

    2015-05-29

    Human adipose-derived stem cells (hASCs) are multipotent progenitor cells with multi-lineage differentiation potential including osteogenesis and adipogenesis. While significant progress has been made in understanding the transcriptional control of hASC fate, little is known about how hASC differentiation is regulated by the autocrine loop. The most abundant adipocytokine secreted by adipocytes, adiponectin (APN) plays a pivotal role in glucose metabolism and energy homeostasis. Growing evidence suggests a positive association between APN and bone formation yet little is known regarding the direct effects of APN on hASC osteogenesis. Therefore, this study was designed to investigate the varied osteogenic effects and regulatory mechanisms of APN in the osteogenic commitment of hASCs. We found that APN enhanced the expression of osteoblast-related genes in hASCs, such as osteocalcin, alkaline phosphatase, and runt-related transcription factor-2 (Runx2, also known as CBFa1), in a dose- and time-dependent manner. This was further confirmed by the higher expression levels of alkaline phosphatase and increased formation of mineralization nodules, along with the absence of inhibition of cell proliferation. Importantly, APN at 1 μg/ml was the optimal concentration, resulting in maximum deposition of calcium nodules, and was significant superior to bone morphogenetic protein 2. Mechanistically, we found for the first time that APN increased nuclear translocation of the leucine zipper motif (APPL)-1 as well as AMP-activated protein kinase (AMPK) phosphorylation, which were reversed by pretreatment with APPL1 siRNA. Our results indicate that APN promotes the osteogenic differentiation of hASCs by activating APPL1-AMPK signaling, suggesting that manipulation of APN is a novel therapeutic target for controlling hASC fate. - Highlights: • Adiponectin enhances osteogenic differentiation in human adipose-derived stem cells. • The knock-down of APPL1 block the enhancement of

  10. PERAN GENDER DALAM PENINGKATAN KESEJAHTERAAN MASYARAKAT DESA WISATA COLO KABUPATEN KUDUS

    Directory of Open Access Journals (Sweden)

    Deny Aditya Puspasari

    2015-11-01

    Full Text Available Development of Village tourism development model is initiated by the Ministry of tourism and Creative Economy for poverty in rural paved. It's what makes Holy County Government initiative to develop the village into a tourist village Colo based nature tourism. Sunan Muria with fame in Kudus, making the village a Colo is located in the mountains of Muria as one of the tourist destinations that continues to evolve. See this potential, the villagers do tourism management in non-governmental and informal communities through the establishment of supporting tourism, such as taxi, hawkers and street vendors. There is a unique phenomena that are contained in the village of Colo namely management of tours with gender roles. Gender roles are not only happening in the improvement of well-being. Be interesting to examined how gender roles towards the improvement of the welfare of the Community tourism village Colo? to answer that question, this research uses a quantitative approach through descriptive statistics methods. Data were collected through document review, interviews and questionnaire to 134 KK as respondents. The results showed that gender roles related to the improvement of the welfare of the community. There is a pattern of increasing well-being of informally through changes in livelihood and ultimately increase revenue.

  11. Analisis Dampak Scouring Pada Integritas Jacket Structure Dengan Pendekatan Statis Berbasis Keandalan

    Directory of Open Access Journals (Sweden)

    Edit Prihantika

    2013-09-01

    Full Text Available Integritas jacket structure sangat terpengaruh dengan kondisi lingkungan yang dapat menimbulkan masalah tertentu, seperti masalah scouring pada jacket. Jacket APN-A adalah salah satu platform yang mengalami scouring dengan rentan masalah yang cukup dikatakan paling parah. Analisa In-place yang harus dilakukan untuk mengetahui kekuatan struktur dengan masalah scouring adalah dengan menghitung unity check pada member dan safety factor pada bearing capacity. Variasi skenario yang dilakukan untuk menganalisa kekuatan jacket APN-A adalah dengan menvariasikan kedalaman scouring hingga 5 meter dan dengan menvariasikan penambahan beban dead load dan live load hingga 30% dari beban awal. Hasil analisa didapatkan bahwa Jacket APN-A tidak terjadi tegangan yang melebihi batas ketentuan ketika mengalami skenario scouring hingga 5 meter, akan tetapi mulai terjadi tegangan yang melebihi batas ketentuan ketika mengalami penambahan beban 10% yaitu pada member 3 yang termasuk Jacket Brace dengan angka mencapai 1,0031. Bearing capacity pada Jacket APN-A, hasil analisa menunjukkan tidak ada yang melebihi kapaitas Safety Factor yang diijinkan ketika mengalami skenario scouring hingga 5 meter, akan tetapi pondasi tidak memenuhi syarat ketika mengalami skenario penambahan beban hingga 20% dari beban awal dengan angka mencapai 1,7. Keandalan dari platform APN-A mengalami penurunan yang cukup siknifikan ketika mengalami penambahan dead load dan live load.

  12. Removal of Malachite Green from water using hydrothermally carbonized pine needles

    KAUST Repository

    Hammud, Hassan Hasan

    2015-01-01

    Hydrothermal carbonization of pine needles (HTC-PN) and their oxidized-activated form HTC-APN are prepared and applied for the adsorption of Malachite Green (MG) in aqueous solution. The HTC materials were characterized by thermal and TEM analysis. Adsorbent dose, initial concentration of MG, contact time, temperature and pH effect on MG adsorption onto the HTC materials were studied. The adsorption equilibrium data was best fitted by the Langmuir isotherm model and the adsorption kinetics followed pseudo-second-order models for both HTC-PN and HTC-APN. The maximum capacity predicted by the Langmuir nonlinear model is 52.91 and 97.08 mg g-1 for uptake of MG by HTC-PN and HTC-APN, respectively, at 30 °C. Thermodynamic investigations showed that the adsorption is spontaneous and endothermic in nature. Results suggest HTC-APN can be used as a low-cost adsorbent for MG removal from industrial wastewater. Yoon-Nelson is the best model with a column capacity of 38.3 mg g-1 for the adsorption of MG onto HTC-APN. This journal is

  13. TNF-alpha, produced by feline infectious peritonitis virus (FIPV)-infected macrophages, upregulates expression of type II FIPV receptor feline aminopeptidase N in feline macrophages.

    Science.gov (United States)

    Takano, Tomomi; Hohdatsu, Tsutomu; Toda, Ayako; Tanabe, Maki; Koyama, Hiroyuki

    2007-07-20

    The pathogenicity of feline infectious peritonitis virus (FIPV) is known to depend on macrophage tropism, and this macrophage infection is enhanced by mediation via anti-S antibody (antibody-dependent enhancement, ADE). In this study, we found that TNF-alpha production was increased with viral replication in macrophages inoculated with a mixture of FIPV and anti-S antibody, and demonstrated that this culture supernatant had feline PBMC apoptosis-inducing activity. We also demonstrated that the expression level of the FIPV virus receptor, feline aminopeptidase N (fAPN), was increased in macrophages of FIP cats. For upregulation of TNF-alpha and fAPN in macrophages, viral replication in macrophages is necessary, and their expressions were increased by ADE of FIPV infection. It was demonstrated that a heat-resistant fAPN-inducing factor was present in the culture supernatant of FIPV-infected macrophages, and this factor was TNF-alpha: fAPN expression was upregulated in recombinant feline TNF-alpha-treated macrophages, and FIPV infectivity was increased in these macrophages. These findings suggested that FIPV replication in macrophages increases TNF-alpha production in macrophages, and the produced TNF-alpha acts and upregulates fAPN expression, increasing FIPV sensitivity.

  14. BISA ULAR MENINGKATKAN DAYA FAGOSITOSIS MAKROFAG PERITONEUM MENCIT TERHADAP KUMAN STREPTOKOKUS GRUP B SNAKE VENOM ENHANCES THE PHAGOCYTIC CAPABILITY OF PERITONEAL MACROPHAGES OF MICE AGAINST THE GROUP B STREPTOCOCCUS

    Directory of Open Access Journals (Sweden)

    Chandramaya Siska Damayanti

    2007-06-01

    Full Text Available Penelitian ini bertujuan mengetahui efek pemberian bisa ular tanah (Calloselasma rhodostoma, ular kobra (Naja naja sputatrix, dan ular welang Bungarus fasciatus pada daya fagositosis makrofag peritoneal mencit terhadap kuman streptokokus grup B. Mencit diberi berbagai dosis bisa ular secara peroral selama 7 hari atau secara intravena sebanyak 3 kali dengan selang waktu penyuntikan 3 hari sekali. Di akhir percobaan

  15. MONITORING TETESAN INFUS BERBASIS MIKROKONTROLER ATMEGA16

    Directory of Open Access Journals (Sweden)

    Ardiyanto Iqbal Nugroho

    2015-09-01

    Penelitian ini menghasilkan suatu alat monitoring tetesan infus yang dapat memberikan informasi mengenai laju kecepatan tetesan dan kondisi cairan pada infus. Sistem yang secara realtime dimonitoring oleh perawat ini dapat mengurangi permasalahan yang timbul karena kelalaian petugas. Sehingga perawat tidak secara manual dalam mengatur kecepatan tetesan infus dan meningkatkan pelayanan kepada pasien.

  16. PENULISAN SEJARAH SOSIAL

    Directory of Open Access Journals (Sweden)

    HARIANTI Harianti

    2015-11-01

    sial, studi tentang kota perkotaan atau sejarah sosial kota dan sebagainya. Langkah-langkah penulisan sejarah sosial meliputi empat kegiatan pokok yakni : Heuristik, Kritik, lnterpretasi dan Penyajian. Penulisan sejarah sosial tidak ditulis secara diskriptif, melainkan harus disusun secara analisis agar merupakan penulisan sejarah yang bersifat ilmiah.

  17. PEMBERDAYAAN PETANI MELALUI PENGUATAN MODAL KELEMBAGAAN PETANI DI KAWASAN AGROPOLITAN KECAMATAN BELIK KABUPATEN PEMALANG (FARMER EMPOWERMENT THROUGH CAPITAL REINFORCEMENT OF FARMERS INSTITUTION AT AGROPOLITAN AREA OF BELIK SUB DISTRICT, PEMALANG REGE)

    OpenAIRE

    Watemin; Sulistyani Budiningsih

    2015-01-01

    Penelitian ini bertujuan untuk mengetahui masalah permodalan dalam usahatani tanaman sayuran di Kecamatan Belik Kabupaten Pemalang. Pemilihan lokasi penelitian dilakukan secara purposive sampling dengan pertimbangan bahwa Kecamatan Belik menjadi pusat bagi pengembangan kawasan agropolitan. Penelitian dilakukan dengan menggunakan metode survey terhadap 12 kelompok tani dan 60 responden petani yang dipilih secara purposive sampling. Data yang terkumpul selanjutnya dianalisis dengan menggunakan...

  18. Kapasitas Jerap Niosom Terhadap Ketoprofen dan Perdiksi Penggunaan Transdermal

    OpenAIRE

    Rahman, Latifah; Ismail, Isriani; Wahyudin, Elly

    2013-01-01

    Niosom adalah system vesikel yang dapat digunakan sebagai pembawa obat lipofilik, hidrofilik dan ampifilik. Ketoprofen adalah salah satu golongan AINS yang sangat sukar larut dalam air dan dapat menyebabkan iritasi lambung pada penggunaan oral. System penghantaran obat secara transdermal sangat penting dikembangkan untuk ketoprofen. Penelitian ini bertujuan untuk memformulasi niosom yang dapat menjerap ketoprofen secara optimal dan dapat menghantarkan ketoprofen melalui rute pemberian transde...

  19. Biologi Radiasi

    OpenAIRE

    Milla Yoesfianda

    2008-01-01

    Biologi radiasi adalah ilmu yang mempelajari tentang pengaruh dari ionisasi radiasi dalam tubuh makhluk hidup. Kemungkinan terjadinya efek biologis akibat interaksi radiasi dan jaringan tubuh manusia, berbanding lurus dengan besarnya dosis radiasi yang mengenai jaringan tubuh tersebut. Radiasi dapat mengakibatkan efek baik secara langsung maupun tidak langsung. Efek yang merusak secara biologis dari radiasi ionisasi diklasifikasikan menjadi tiga kategori utama, yaitu efek somatik determin...

  20. Aplikasi Short Message Service (SMS dan Email Sebagai Media Komunikasi Data dalam Sistem Pemantauan Suhu

    Directory of Open Access Journals (Sweden)

    Andi Adriansyah

    2011-06-01

    Full Text Available      Sistem pemantauan suhu secara langsung dengan mencatat setiap perubahan suhu secara manual, terlebih pada kondisi suhu yang ekstrim, memiliki resiko yang dapat berakibat fatal. Oleh karena itu dibutuhkan sebuah sistem yang dapat melakukannya secara otomatis. Penelitian ini bertujuan merancang sebuah sistem mandiri yang dapat melakukan pemantauan dan pencatatan perubahan suhu secara near real time (NRT dan mengirimkan laporan secara otomatis melalui email dan SMS. Sistem terdiri dari Sensor suhu LM35 yang berfungsi merubah besaran fisis suhu ke besaran listrik tegangan, Microcontroller Atmega8535 yang memegang peranan untuk mengkonversi tegangan keluaran LM35 kedalam bentuk digital, serta mengirimkan data hasil konversi melalui komunikasi serial ke computer. Program Java yang dijalankan di computer berfungsi memproses data yang dikirim mikrokontroler ATmega8535, menyimpannya ke basis data MySQL, menampilkannya dalam bentuk tabel dan grafik, dan mengirimkan laporan melalui email dan SMS bila data suhu yang diterima melebihi ambang batas yang telah ditentukan.

  1. Cloning of aminopeptidase Npromoter and its activity in hematopoietic cell and different tumor cell lines

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Aminopeptidase N (APN) promoter region was cloned and sequenced from peripheral blood mononuclear cells. The recombinant reporter construct containing the promoter and luciferase gene, designated pXP1-APNLuc, was introduced into myeloblastic cell line, T lymphocyte cell line and various tumor cell lines. Luciferase assay showed that APN upstream promoter is myeloid-specific for high expression in myeloblastic cell line and much lower expres sion in T lymphocyte cell line. The promoter activity was relatively high in lung adenoma cell line compared with other tumor cell lines including hepatoma cell line, tong cancer cell line and esophageal cancer cell line in which the promoter activity significantly diminished or was almost undetectable. The characteristics of APN promoter may pro vide a new strategy for specific myeloprotection while tumor patients are being treated with chemotherapy and/or radio therapy.

  2. Gerontology found me: gaining understanding of advanced practice nurses in geriatrics.

    Science.gov (United States)

    Campbell-Detrixhe, Dia D; Grassley, Jane S; Zeigler, Vicki L

    2013-10-01

    Examining the meanings of the experiences of advanced practice nurses (APNs) who chose to work with older adults and why they continue to work with this population was the focus of this hermeneutic qualitative research study. Twelve geriatric APNs currently practicing in two South Central states were interviewed using an open-ended interview guide. Using Gadamerian hermeneutics, the researchers identified Gerontology Found Me as the significant expression that reflected the fundamental meaning of the experience as a whole. Four themes emerged that further described the meanings of the participants' personal, educational, and professional experiences: Becoming a Gerontology Nurse, Being a Gerontology Nurse, Belonging to Gerontology, and Bringing Others to Gerontology. This study concluded that APNs' personal and professional experiences were more influential than educational experiences to become geriatric nurses, and having these personal and professional experiences of being in relationship with older individuals further contributed to their choice of gerontology.

  3. Improving Awareness, Identification, and Management of Sarcopenic Obesity in Cancer Survivors: An Evidence-Based Toolbox.

    Science.gov (United States)

    Lindsey, Sarah; Astroth, Kim; Kumar, Pankaj

    2016-10-01

    Sarcopenic obesity, the dual condition of decreased muscle mass with increased fat mass, can affect morbidity, mortality, and quality of life in adult cancer survivors. The purpose of this project was to determine the effects of the use of an educational toolbox on advanced practice nurses' (APNs') confidence in identifying and managing adult cancer survivors at risk for sarcopenic obesity. APNs in an outpatient practice who care for adult cancer survivors received an educational toolbox with strategies to identify and manage adult cancer survivors at risk for sarcopenic obesity. APNs reported being more confident in their ability to identify adult patients with cancer at risk for sarcopenic obesity and in their ability to manage these patients compared to prior to the intervention. Educational resources provided an effective tool for identifying and managing patients at risk for sarcopenic obesity.

  4. Plasma Adiponectin Levels in Acute Liver Failure Patients Treated with Plasma Filtration with Dialysis and Plasma Exchange.

    Science.gov (United States)

    Yamamoto, Hiroshi; Nakae, Hajime; Uji, Yoshitaka; Maeda, Kazuhisa; Tani, Tohru; Eguchi, Yutaka

    2015-08-01

    Plasma filtration with dialysis (PDF) is a blood purification therapy in which simple plasma exchange (PE) is performed using a selective membrane plasma separator while the dialysate flows outside of the hollow fibers. Improvement of hypoadiponectinemia is considered to be a useful therapeutic approach for ameliorating fatal conditions including cardio-metabolic and infectious disease. We investigated the effects of PDF in comparison to PE in terms of plasma adiponectin (APN) changes in patients with acute liver failure. Seventeen patients with liver failure were studied; PDF was performed 55 times and PE 14 times. Plasma APN levels increased significantly after PDF, while decreasing significantly after PE. PDF appears to be among the most useful blood purification therapies in acute liver failure cases in terms of increasing APN levels.

  5. Oximetry alone versus portable polygraphy for sleep apnea screening before bariatric surger

    OpenAIRE

    Malbois, M.

    2010-01-01

    Rapport de SynthèseIntroductionLa recherche des apnées du sommeil est recommandée dans la prise en charge préopératoire des patients obèses chez qui une chirurgie bariatrique est envisagée. Toutefois le type d'examen nécessaire pour la détection des apnées dans cette population reste encore discuté. L'objectif de cette étude était de comparer la sensibilité de l'oxymétrie par rapport à la polygraphie lors du screening préopératoire de l'apnée obstructive du sommeil.MéthodeNous avons analysé r...

  6. Biologic correlates to the development of post-traumatic stress disorder in female victims of intimate partner violence: implications for practice.

    Science.gov (United States)

    Scott-Tilley, Donna; Tilton, Abigail; Sandel, Mark

    2010-01-01

    The purpose of this article is to discuss the current literature about the risk of post-traumatic stress disorder (PTSD) in victims of intimate partner violence (IPV). PTSD is a complex and serious syndrome with emotional symptoms, psychiatric symptoms, and physical consequences. PTSD is associated with impaired immune function, obesity, increased risk of diabetes, increased severity of premenstrual syndrome symptoms, depression, suicide, and increased likelihood of re-abuse. Female victims of IPV develop PTSD and related consequences at a rate of 74-92%, compared to 6-13% in non-abused women. Advanced practice nurses (APNs) are likely to see one or both of these problems in their practices. The vague, nonspecific symptoms can present challenges to APNs. Biologic correlates are identified to help the APNs identify those most at risk of developing PTSD. Treatment options are discussed.

  7. Developing grant writing skills to translate practice dreams into reality.

    Science.gov (United States)

    Lindquist, Ruth; Hadidi, Niloufar

    2013-01-01

    In an era of health care reform and limited financial support, good ideas for changes in clinical practice may await the available time, resources, and attention that are required to test and implement them. Developing grant writing skills is a way to attract resources to explore the feasibility and potential efficacy of changes to improve patient outcomes or efficiencies of care. This article describes the purpose of grant writing by advanced practice nurses (APNs), discusses the needs for and benefits of grant writing, identifies types and sources of available grants, describes potential roles of APNs in grant writing, describes ways to overcome barriers to grant writing, and presents strategies for writing winning grants to develop and improve practice in acute and critical care settings. These strategies will help APNs get started and provide a guide to follow in writing their first grant or will refresh their existing grant writing skills.

  8. A latency analysis for M2M and OG-like traffic patterns in different HSPA core network configurations

    Directory of Open Access Journals (Sweden)

    M. V. Popović

    2014-11-0