WorldWideScience

Sample records for multiphoton 3d imaging

  1. Multiphotonic Confocal Microscopy 3D imaging: Application to mantle sulfides in sub-arc environment (Avacha Volcano, Kamchatka)

    Science.gov (United States)

    Antoine, Bénard; Luc-Serge, Doucet; Sabine, Palle; Dmitri A., Ionov

    2010-05-01

    Petrogenetic relations in igneous rocks are usually studied in natural samples using classical optical microscopy and subsequent geochemical data acquisition. Multiphotonic Laser Scanning Confocal Microscopy (MLSCM) can be a powerful tool to section geological materials optically with sub-micrometric resolution and then generate a three-dimensional (3D) reconstruction (ca. 106 μm3 stack). MLSCM is used here to investigate textural relations of Monosulfide Solid Solution (MSS) with silicate phases in fresh spinel harzburgite xenoliths from the andesitic Avacha volcano (Kamchatka, Russia). The xenoliths contain MSS disseminated in olivine and orthopyroxene (opx) neoblasts as well as MSS-rich quenched magmatic opx veins [1]. First, Reflection Mode (RM) was tested on vein sulfides in resin-impregnated thick (120 μm) polished rock sections. Then we used a combination of Differential Interference Contrast (DIC) with a transmitted light detector, two photons-excited fluorescence (2PEF) and Second Harmonic Generation (SHG). Sequential imaging feature of the Leica TCS-SP2 software was applied. The excitation laser used for 2PEF was a COHERENT MIRA 900 with a 76Hz repetition rate and 800nm wavelength. Image stacks were analysed using ImageJ software [2]. The aim of the tests was to try to discriminate sulfides in silicate matrix as a tool for a better assessment of equilibrium conditions between the two phases. Preliminary results show that Fe-Ni rich MSS from vein and host rock have a strong auto-fluorescence in the Near UV-VIS domain (392-715 nm) whereas silicate matrix is only revealed through DIC. SHG is obtained only from dense nanocentrosymmetrical structures such as embedded medium (organic matter like glue and resin). The three images were recorded sequentially enabling efficient discrimination between the different components of the rock slices. RM permits reconstruction of the complete 3D structure of the rock slice. High resolution (ca. 0.2 μm along X-Y axis vs

  2. Quantitative multiphoton imaging

    Science.gov (United States)

    König, Karsten; Weinigel, Martin; Breunig, Hans Georg; Uchugonova, Aisada

    2014-02-01

    Certified clinical multiphoton tomographs for label-free multidimensional high-resolution in vivo imaging have been introduced to the market several years ago. Novel tomographs include a flexible 360° scan head attached to a mechanooptical arm for autofluorescence and SHG imaging as well as a CARS module. Non-fluorescent lipids and water, mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen can be imaged in vivo with submicron resolution in human skin. Sensitive and rapid detectors allow single photon counting and the construction of 3D maps where the number of detected photons per voxel is depicted. Intratissue concentration profiles from endogenous as well exogenous substances can be generated when the number of detected photons can be correlated with the number of molecules with respect to binding and scattering behavior. Furthermore, the skin ageing index SAAID based on the ratio elastin/collagen as well as the epidermis depth based on the onset of SHG generation can be determined.

  3. Multiphoton Microscopy for Ophthalmic Imaging

    Directory of Open Access Journals (Sweden)

    Emily A. Gibson

    2011-01-01

    Full Text Available We review multiphoton microscopy (MPM including two-photon autofluorescence (2PAF, second harmonic generation (SHG, third harmonic generation (THG, fluorescence lifetime (FLIM, and coherent anti-Stokes Raman Scattering (CARS with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.

  4. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    Science.gov (United States)

    Lee, Seunghun; Lee, Jun Ho; Park, Jin Hyoung; Yoon, Yeoreum; Chung, Wan Kyun; Tchah, Hungwon; Kim, Myoung Joon; Kim, Ki Hean

    2016-05-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. Intrinsic multiphoton fluorescence properties of moxifloxacin and gatifloxacin were characterized, and their distributions in mouse cornea in vivo were measured by 3D MPM imaging. Both moxifloxacin and gatifloxacin had similar multiphoton spectra, while moxifloxacin had stronger fluorescence than gatifloxacin. MPM imaging of mouse cornea in vivo showed (1) moxifloxacin had good penetration through the superficial corneal epithelium, while gatifloxacin had relatively poor penetration, (2) both ophthalmic solutions had high intracellular distribution. In vivo MPM results were consistent with previous studies. This study demonstrates the feasibility of MPM as a method for in vivo direct measurement of moxifloxacin and gatifloxacin in the cornea.

  5. Advanced 3-D Ultrasound Imaging

    DEFF Research Database (Denmark)

    Rasmussen, Morten Fischer

    The main purpose of the PhD project was to develop methods that increase the 3-D ultrasound imaging quality available for the medical personnel in the clinic. Acquiring a 3-D volume gives the medical doctor the freedom to investigate the measured anatomy in any slice desirable after the scan has...... been completed. This allows for precise measurements of organs dimensions and makes the scan more operator independent. Real-time 3-D ultrasound imaging is still not as widespread in use in the clinics as 2-D imaging. A limiting factor has traditionally been the low image quality achievable using...... a channel limited 2-D transducer array and the conventional 3-D beamforming technique, Parallel Beamforming. The first part of the scientific contributions demonstrate that 3-D synthetic aperture imaging achieves a better image quality than the Parallel Beamforming technique. Data were obtained using both...

  6. 3D vector flow imaging

    DEFF Research Database (Denmark)

    Pihl, Michael Johannes

    The main purpose of this PhD project is to develop an ultrasonic method for 3D vector flow imaging. The motivation is to advance the field of velocity estimation in ultrasound, which plays an important role in the clinic. The velocity of blood has components in all three spatial dimensions, yet...... are (vx, vy, vz) = (-0.03, 95, 1.0) ± (9, 6, 1) cm/s compared with the expected (0, 96, 0) cm/s. Afterwards, 3D vector flow images from a cross-sectional plane of the vessel are presented. The out of plane velocities exhibit the expected 2D circular-symmetric parabolic shape. The experimental results...... verify that the 3D TO method estimates the complete 3D velocity vectors, and that the method is suitable for 3D vector flow imaging....

  7. Multiphoton microscopy imaging of developing tooth germs

    Directory of Open Access Journals (Sweden)

    Pei-Yu Pan

    2014-01-01

    Conclusion: In this study, a novel multiphoton microscopy database of images from developing tooth germs in mice was set up. We confirmed that multiphoton laser microscopy is a powerful tool for investigating the development of tooth germ and is worthy for further application in the study of tooth regeneration.

  8. Multiphoton crosslinking for biocompatible 3D printing of type I collagen.

    Science.gov (United States)

    Bell, Alex; Kofron, Matthew; Nistor, Vasile

    2015-09-03

    Multiphoton fabrication is a powerful technique for three-dimensional (3D) printing of structures at the microscale. Many polymers and proteins have been successfully structured and patterned using this method. Type I collagen comprises a large part of the extracellular matrix for most tissue types and is a widely used cellular scaffold material for tissue engineering. Current methods for creating collagen tissue scaffolds do not allow control of local geometry on a cellular scale. This means the environment experienced by cells may be made up of the native material but unrelated to native cellular-scale structure. In this study, we present a novel method to allow multiphoton crosslinking of type I collagen with flavin mononucleotide photosensitizer. The method detailed allows full 3D printing of crosslinked structures made from unmodified type I collagen and uses only demonstrated biocompatible materials. Resolution of 1 μm for both standing lines and high-aspect ratio gaps between structures is demonstrated and complex 3D structures are fabricated. This study demonstrates a means for 3D printing with one of the most widely used tissue scaffold materials. High-resolution, 3D control of the fabrication of collagen scaffolds will facilitate higher fidelity recreation of the native extracellular environment for engineered tissues.

  9. Analysis of 3-D images

    Science.gov (United States)

    Wani, M. Arif; Batchelor, Bruce G.

    1992-03-01

    Deriving generalized representation of 3-D objects for analysis and recognition is a very difficult task. Three types of representations based on type of an object is used in this paper. Objects which have well-defined geometrical shapes are segmented by using a fast edge region based segmentation technique. The segmented image is represented by plan and elevation of each part of the object if the object parts are symmetrical about their central axis. The plan and elevation concept enables representing and analyzing such objects quickly and efficiently. The second type of representation is used for objects having parts which are not symmetrical about their central axis. The segmented surface patches of such objects are represented by the 3-D boundary and the surface features of each segmented surface. Finally, the third type of representation is used for objects which don't have well-defined geometrical shapes (for example a loaf of bread). These objects are represented and analyzed from its features which are derived using a multiscale contour based technique. Anisotropic Gaussian smoothing technique is introduced to segment the contours at various scales of smoothing. A new merging technique is used which enables getting the current best estimate of break points at each scale. This new technique enables elimination of loss of accuracy of localization effects at coarser scales without using scale space tracking approach.

  10. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    OpenAIRE

    Lee, Seunghun; Lee, Jun Ho; Park, Jin Hyoung; Yoon, Yeoreum; Chung, Wan Kyun; Tchah, Hungwon; Kim, Myoung Joon; Kim, Ki Hean

    2016-01-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. I...

  11. 3-D Vector Flow Imaging

    DEFF Research Database (Denmark)

    Holbek, Simon

    , if this significant reduction in the element count can still provide precise and robust 3-D vector flow estimates in a plane. The study concludes that the RC array is capable of estimating precise 3-D vector flow both in a plane and in a volume, despite the low channel count. However, some inherent new challenges...... ultrasonic vector flow estimation and bring it a step closer to a clinical application. A method for high frame rate 3-D vector flow estimation in a plane using the transverse oscillation method combined with a 1024 channel 2-D matrix array is presented. The proposed method is validated both through phantom...... hampers the task of real-time processing. In a second study, some of the issue with the 2-D matrix array are solved by introducing a 2-D row-column (RC) addressing array with only 62 + 62 elements. It is investigated both through simulations and via experimental setups in various flow conditions...

  12. 3D images and expert system

    International Nuclear Information System (INIS)

    Hasegawa, Jun-ichi

    1998-01-01

    This paper presents an expert system called 3D-IMPRESS for supporting applications of three dimensional (3D) image processing. This system can automatically construct a 3D image processing procedure based on a pictorial example of the goal given by a user. In the paper, to evaluate the performance of the system, it was applied to construction of procedures for extracting specific component figures from practical chest X-ray CT images. (author)

  13. 3D molecular imaging SIMS

    Energy Technology Data Exchange (ETDEWEB)

    Gillen, Greg [Surface and Microanalysis Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8371 (United States)]. E-mail: Greg.gillen@nist.gov; Fahey, Albert [Surface and Microanalysis Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8371 (United States); Wagner, Matt [Surface and Microanalysis Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8371 (United States); Mahoney, Christine [Surface and Microanalysis Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8371 (United States)

    2006-07-30

    Thin monolayer and bilayer films of spin cast poly(methyl methacrylate) (PMMA), poly(2-hydroxyethyl methacrylate) (PHEMA), poly(lactic) acid (PLA) and PLA doped with several pharmaceuticals have been analyzed by dynamic SIMS using SF{sub 5} {sup +} polyatomic primary ion bombardment. Each of these systems exhibited minimal primary beam-induced degradation under cluster ion bombardment allowing molecular depth profiles to be obtained through the film. By combing secondary ion imaging with depth profiling, three-dimensional molecular image depth profiles have been obtained from these systems. In another approach, bevel cross-sections are cut in the samples with the SF{sub 5} {sup +} primary ion beam to produce a laterally magnified cross-section of the sample that does not contain the beam-induced damage that would be induced by conventional focussed ion beam (FIB) cross-sectioning. The bevel surface can then be examined using cluster SIMS imaging or other appropriate microanalysis technique.

  14. 3D Backscatter Imaging System

    Science.gov (United States)

    Whitaker, Ross (Inventor); Turner, D. Clark (Inventor)

    2016-01-01

    Systems and methods for imaging an object using backscattered radiation are described. The imaging system comprises both a radiation source for irradiating an object that is rotationally movable about the object, and a detector for detecting backscattered radiation from the object that can be disposed on substantially the same side of the object as the source and which can be rotationally movable about the object. The detector can be separated into multiple detector segments with each segment having a single line of sight projection through the object and so detects radiation along that line of sight. Thus, each detector segment can isolate the desired component of the backscattered radiation. By moving independently of each other about the object, the source and detector can collect multiple images of the object at different angles of rotation and generate a three dimensional reconstruction of the object. Other embodiments are described.

  15. 3D composite image, 3D MRI, 3D SPECT, hydrocephalus

    International Nuclear Information System (INIS)

    Mito, T.; Shibata, I.; Sugo, N.; Takano, M.; Takahashi, H.

    2002-01-01

    The three-dimensional (3D)SPECT imaging technique we have studied and published for the past several years is an analytical tool that permits visual expression of the cerebral circulation profile in various cerebral diseases. The greatest drawback of SPECT is that the limitation on precision of spacial resolution makes intracranial localization impossible. In 3D SPECT imaging, intracranial volume and morphology may vary with the threshold established. To solve this problem, we have produced complimentarily combined SPECT and helical-CT 3D images by means of general-purpose visualization software for intracranial localization. In hydrocephalus, however, the key subject to be studied is the profile of cerebral circulation around the ventricles of the brain. This suggests that, for displaying the cerebral ventricles in three dimensions, CT is a difficult technique whereas MRI is more useful. For this reason, we attempted to establish the profile of cerebral circulation around the cerebral ventricles by the production of combined 3D images of SPECT and MRI. In patients who had shunt surgery for hydrocephalus, a difference between pre- and postoperative cerebral circulation profiles was assessed by a voxel distribution curve, 3D SPECT images, and combined 3D SPECT and MRI images. As the shunt system in this study, an Orbis-Sigma valve of the automatic cerebrospinal fluid volume adjustment type was used in place of the variable pressure type Medos valve currently in use, because this device requires frequent changes in pressure and a change in pressure may be detected after MRI procedure. The SPECT apparatus used was PRISM3000 of the three-detector type, and 123I-IMP was used as the radionuclide in a dose of 222 MBq. MRI data were collected with an MAGNEXa+2 with a magnetic flux density of 0.5 tesla under the following conditions: field echo; TR 50 msec; TE, 10 msec; flip, 30ueK; 1 NEX; FOV, 23 cm; 1-mm slices; and gapless. 3D images are produced on the workstation TITAN

  16. Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media.

    Science.gov (United States)

    Sugita, Shukei; Matsumoto, Takeo

    2017-06-01

    Elastin and collagen fibers play important roles in the mechanical properties of aortic media. Because knowledge of local fiber structures is required for detailed analysis of blood vessel wall mechanics, we investigated 3D microstructures of elastin and collagen fibers in thoracic aortas and monitored changes during pressurization. Using multiphoton microscopy, autofluorescence images from elastin and second harmonic generation signals from collagen were acquired in media from rabbit thoracic aortas that were stretched biaxially to restore physiological dimensions. Both elastin and collagen fibers were observed in all longitudinal-circumferential plane images, whereas alternate bright and dark layers were observed along the radial direction and were recognized as elastic laminas (ELs) and smooth muscle-rich layers (SMLs), respectively. Elastin and collagen fibers are mainly oriented in the circumferential direction, and waviness of collagen fibers was significantly higher than that of elastin fibers. Collagen fibers were more undulated in longitudinal than in radial direction, whereas undulation of elastin fibers was equibiaxial. Changes in waviness of collagen fibers during pressurization were then evaluated using 2-dimensional fast Fourier transform in mouse aortas, and indices of waviness of collagen fibers decreased with increases in intraluminal pressure. These indices also showed that collagen fibers in SMLs became straight at lower intraluminal pressures than those in EL, indicating that SMLs stretched more than ELs. These results indicate that deformation of the aorta due to pressurization is complicated because of the heterogeneity of tissue layers and differences in elastic properties of ELs, SMLs, and surrounding collagen and elastin.

  17. 3D Membrane Imaging and Porosity Visualization

    KAUST Repository

    Sundaramoorthi, Ganesh; Hadwiger, Markus; Ben Romdhane, Mohamed; Behzad, Ali Reza; Madhavan, Poornima; Nunes, Suzana Pereira

    2016-01-01

    Ultrafiltration asymmetric porous membranes were imaged by two microscopy methods, which allow 3D reconstruction: Focused Ion Beam and Serial Block Face Scanning Electron Microscopy. A new algorithm was proposed to evaluate porosity and average pore

  18. 3D Reconstruction of NMR Images

    Directory of Open Access Journals (Sweden)

    Peter Izak

    2007-01-01

    Full Text Available This paper introduces experiment of 3D reconstruction NMR images scanned from magnetic resonance device. There are described methods which can be used for 3D reconstruction magnetic resonance images in biomedical application. The main idea is based on marching cubes algorithm. For this task was chosen sophistication method by program Vision Assistant, which is a part of program LabVIEW.

  19. Nonlaser-based 3D surface imaging

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Shin-yee; Johnson, R.K.; Sherwood, R.J. [Lawrence Livermore National Lab., CA (United States)

    1994-11-15

    3D surface imaging refers to methods that generate a 3D surface representation of objects of a scene under viewing. Laser-based 3D surface imaging systems are commonly used in manufacturing, robotics and biomedical research. Although laser-based systems provide satisfactory solutions for most applications, there are situations where non laser-based approaches are preferred. The issues that make alternative methods sometimes more attractive are: (1) real-time data capturing, (2) eye-safety, (3) portability, and (4) work distance. The focus of this presentation is on generating a 3D surface from multiple 2D projected images using CCD cameras, without a laser light source. Two methods are presented: stereo vision and depth-from-focus. Their applications are described.

  20. 3D EIT image reconstruction with GREIT.

    Science.gov (United States)

    Grychtol, Bartłomiej; Müller, Beat; Adler, Andy

    2016-06-01

    Most applications of thoracic EIT use a single plane of electrodes on the chest from which a transverse image 'slice' is calculated. However, interpretation of EIT images is made difficult by the large region above and below the electrode plane to which EIT is sensitive. Volumetric EIT images using two (or more) electrode planes should help compensate, but are little used currently. The Graz consensus reconstruction algorithm for EIT (GREIT) has become popular in lung EIT. One shortcoming of the original formulation of GREIT is its restriction to reconstruction onto a 2D planar image. We present an extension of the GREIT algorithm to 3D and develop open-source tools to evaluate its performance as a function of the choice of stimulation and measurement pattern. Results show 3D GREIT using two electrode layers has significantly more uniform sensitivity profiles through the chest region. Overall, the advantages of 3D EIT are compelling.

  1. Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair.

    Science.gov (United States)

    Li, Jian; Jahr, Holger; Zheng, Wei; Ren, Pei-Gen

    2017-09-07

    The reconstruction of critically sized bone defects remains a serious clinical problem because of poor angiogenesis within tissue-engineered scaffolds during repair, which gives rise to a lack of sufficient blood supply and causes necrosis of the new tissues. Rapid vascularization is a vital prerequisite for new tissue survival and integration with existing host tissue. The de novo generation of vasculature in scaffolds is one of the most important steps in making bone regeneration more efficient, allowing repairing tissue to grow into a scaffold. To tackle this problem, the genetic modification of a biomaterial scaffold is used to accelerate angiogenesis and osteogenesis. However, visualizing and tracking in vivo blood vessel formation in real-time and in three-dimensional (3D) scaffolds or new bone tissue is still an obstacle for bone tissue engineering. Multiphoton microscopy (MPM) is a novel bio-imaging modality that can acquire volumetric data from biological structures in a high-resolution and minimally-invasive manner. The objective of this study was to visualize angiogenesis with multiphoton microscopy in vivo in a genetically modified 3D-PLGA/nHAp scaffold for calvarial critical bone defect repair. PLGA/nHAp scaffolds were functionalized for the sustained delivery of a growth factor pdgf-b gene carrying lentiviral vectors (LV-pdgfb) in order to facilitate angiogenesis and to enhance bone regeneration. In a scaffold-implanted calvarial critical bone defect mouse model, the blood vessel areas (BVAs) in PHp scaffolds were significantly higher than in PH scaffolds. Additionally, the expression of pdgf-b and angiogenesis-related genes, vWF and VEGFR2, increased correspondingly. MicroCT analysis indicated that the new bone formation in the PHp group dramatically improved compared to the other groups. To our knowledge, this is the first time multiphoton microscopy was used in bone tissue-engineering to investigate angiogenesis in a 3D bio-degradable scaffold in

  2. 3-D image reconstruction in radiology

    International Nuclear Information System (INIS)

    Grangeat, P.

    1999-01-01

    In this course, we present highlights on fully 3-D image reconstruction algorithms used in 3-D X-ray Computed Tomography (3-D-CT) and 3-D Rotational Radiography (3-D-RR). We first consider the case of spiral CT with a one-row detector. Starting from the 2-D fan-beam inversion formula for a circular trajectory, we introduce spiral CT 3-D image reconstruction algorithm using axial interpolation for each transverse slice. In order to improve the X-ray detection efficiency and to speed the acquisition process, the future is to use multi-row detectors associated with small angle cone-beam geometry. The generalization of the 2-D fan-beam image reconstruction algorithm to cone beam defined direct inversion formula referred as Feldkamp's algorithm for a circular trajectory and Wang's algorithm for a spiral trajectory. However, large area detectors does exist such as Radiological Image Intensifiers or in a near future solid state detectors. To get a larger zoom effect, it defines a cone-beam geometry associated with a large aperture angle. For this case, we introduce indirect image reconstruction algorithm by plane re-binning in the Radon domain. We will present some results from a prototype MORPHOMETER device using the RADON reconstruction software. Lastly, we consider the special case of 3-D Rotational Digital Subtraction Angiography with a restricted number of views. We introduce constraint optimization algorithm using quadratic, entropic or half-quadratic constraints. Generalized ART (Algebraic Reconstruction Technique) iterative reconstruction algorithm can be derived from the Bregman algorithm. We present reconstructed vascular trees from a prototype MORPHOMETER device. (author)

  3. 3D Printing Openable Imaging Phantom Design

    International Nuclear Information System (INIS)

    Kim, Myoung Keun; Won, Jun Hyeok; Lee, Seung Wook

    2017-01-01

    The purpose of this study is to design an openable phantom that can replace the internal measurement bar used for contrast comparison in order to increase the efficiency of manufacturing imaging phantom used in the medical industry and to improve convenience using 3D printer. Phantom concept design, 3D printing, and Image reconstruction were defined as the scope of the thesis. Also, we study metal artifact reduction with openable phantom. We have designed a Openable phantom using 3D printing, and have investigated metal artifact reduction after inserting a metallic material inside the phantom. The openable phantom can be adjusted at any time to suit the user's experiment and can be easily replaced and useful.

  4. 3D Membrane Imaging and Porosity Visualization

    KAUST Repository

    Sundaramoorthi, Ganesh

    2016-03-03

    Ultrafiltration asymmetric porous membranes were imaged by two microscopy methods, which allow 3D reconstruction: Focused Ion Beam and Serial Block Face Scanning Electron Microscopy. A new algorithm was proposed to evaluate porosity and average pore size in different layers orthogonal and parallel to the membrane surface. The 3D-reconstruction enabled additionally the visualization of pore interconnectivity in different parts of the membrane. The method was demonstrated for a block copolymer porous membrane and can be extended to other membranes with application in ultrafiltration, supports for forward osmosis, etc, offering a complete view of the transport paths in the membrane.

  5. 3D IMAGING USING COHERENT SYNCHROTRON RADIATION

    Directory of Open Access Journals (Sweden)

    Peter Cloetens

    2011-05-01

    Full Text Available Three dimensional imaging is becoming a standard tool for medical, scientific and industrial applications. The use of modem synchrotron radiation sources for monochromatic beam micro-tomography provides several new features. Along with enhanced signal-to-noise ratio and improved spatial resolution, these include the possibility of quantitative measurements, the easy incorporation of special sample environment devices for in-situ experiments, and a simple implementation of phase imaging. These 3D approaches overcome some of the limitations of 2D measurements. They require new tools for image analysis.

  6. Metrological characterization of 3D imaging devices

    Science.gov (United States)

    Guidi, G.

    2013-04-01

    Manufacturers often express the performance of a 3D imaging device in various non-uniform ways for the lack of internationally recognized standard requirements for metrological parameters able to identify the capability of capturing a real scene. For this reason several national and international organizations in the last ten years have been developing protocols for verifying such performance. Ranging from VDI/VDE 2634, published by the Association of German Engineers and oriented to the world of mechanical 3D measurements (triangulation-based devices), to the ASTM technical committee E57, working also on laser systems based on direct range detection (TOF, Phase Shift, FM-CW, flash LADAR), this paper shows the state of the art about the characterization of active range devices, with special emphasis on measurement uncertainty, accuracy and resolution. Most of these protocols are based on special objects whose shape and size are certified with a known level of accuracy. By capturing the 3D shape of such objects with a range device, a comparison between the measured points and the theoretical shape they should represent is possible. The actual deviations can be directly analyzed or some derived parameters can be obtained (e.g. angles between planes, distances between barycenters of spheres rigidly connected, frequency domain parameters, etc.). This paper shows theoretical aspects and experimental results of some novel characterization methods applied to different categories of active 3D imaging devices based on both principles of triangulation and direct range detection.

  7. 3D Seismic Imaging using Marchenko Methods

    Science.gov (United States)

    Lomas, A.; Curtis, A.

    2017-12-01

    Marchenko methods are novel, data driven techniques that allow seismic wavefields from sources and receivers on the Earth's surface to be redatumed to construct wavefields with sources in the subsurface - including complex multiply-reflected waves, and without the need for a complex reference model. In turn, this allows subsurface images to be constructed at any such subsurface redatuming points (image or virtual receiver points). Such images are then free of artefacts from multiply-scattered waves that usually contaminate migrated seismic images. Marchenko algorithms require as input the same information as standard migration methods: the full reflection response from sources and receivers at the Earth's surface, and an estimate of the first arriving wave between the chosen image point and the surface. The latter can be calculated using a smooth velocity model estimated using standard methods. The algorithm iteratively calculates a signal that focuses at the image point to create a virtual source at that point, and this can be used to retrieve the signal between the virtual source and the surface. A feature of these methods is that the retrieved signals are naturally decomposed into up- and down-going components. That is, we obtain both the signal that initially propagated upwards from the virtual source and arrived at the surface, separated from the signal that initially propagated downwards. Figure (a) shows a 3D subsurface model with a variable density but a constant velocity (3000m/s). Along the surface of this model (z=0) in both the x and y directions are co-located sources and receivers at 20-meter intervals. The redatumed signal in figure (b) has been calculated using Marchenko methods from a virtual source (1200m, 500m and 400m) to the surface. For comparison the true solution is given in figure (c), and shows a good match when compared to figure (b). While these 2D redatuming and imaging methods are still in their infancy having first been developed in

  8. Adaptive optics improves multiphoton super-resolution imaging

    Science.gov (United States)

    Zheng, Wei; Wu, Yicong; Winter, Peter; Shroff, Hari

    2018-02-01

    Three dimensional (3D) fluorescence microscopy has been essential for biological studies. It allows interrogation of structure and function at spatial scales spanning the macromolecular, cellular, and tissue levels. Critical factors to consider in 3D microscopy include spatial resolution, signal-to-noise (SNR), signal-to-background (SBR), and temporal resolution. Maintaining high quality imaging becomes progressively more difficult at increasing depth (where optical aberrations, induced by inhomogeneities of refractive index in the sample, degrade resolution and SNR), and in thick or densely labeled samples (where out-of-focus background can swamp the valuable, in-focus-signal from each plane). In this report, we introduce our new instrumentation to address these problems. A multiphoton structured illumination microscope was simply modified to integrate an adpative optics system for optical aberrations correction. Firstly, the optical aberrations are determined using direct wavefront sensing with a nonlinear guide star and subsequently corrected using a deformable mirror, restoring super-resolution information. We demonstrate the flexibility of our adaptive optics approach on a variety of semi-transparent samples, including bead phantoms, cultured cells in collagen gels and biological tissues. The performance of our super-resolution microscope is improved in all of these samples, as peak intensity is increased (up to 40-fold) and resolution recovered (up to 176+/-10 nm laterally and 729+/-39 nm axially) at depths up to 250 μm from the coverslip surface.

  9. Imaging chemical reactions - 3D velocity mapping

    Science.gov (United States)

    Chichinin, A. I.; Gericke, K.-H.; Kauczok, S.; Maul, C.

    Visualising a collision between an atom or a molecule or a photodissociation (half-collision) of a molecule on a single particle and single quantum level is like watching the collision of billiard balls on a pool table: Molecular beams or monoenergetic photodissociation products provide the colliding reactants at controlled velocity before the reaction products velocity is imaged directly with an elaborate camera system, where one should keep in mind that velocity is, in general, a three-dimensional (3D) vectorial property which combines scattering angles and speed. If the processes under study have no cylindrical symmetry, then only this 3D product velocity vector contains the full information of the elementary process under study.

  10. 3D imaging, 3D printing and 3D virtual planning in endodontics.

    Science.gov (United States)

    Shah, Pratik; Chong, B S

    2018-03-01

    The adoption and adaptation of recent advances in digital technology, such as three-dimensional (3D) printed objects and haptic simulators, in dentistry have influenced teaching and/or management of cases involving implant, craniofacial, maxillofacial, orthognathic and periodontal treatments. 3D printed models and guides may help operators plan and tackle complicated non-surgical and surgical endodontic treatment and may aid skill acquisition. Haptic simulators may assist in the development of competency in endodontic procedures through the acquisition of psycho-motor skills. This review explores and discusses the potential applications of 3D printed models and guides, and haptic simulators in the teaching and management of endodontic procedures. An understanding of the pertinent technology related to the production of 3D printed objects and the operation of haptic simulators are also presented.

  11. Photogrammetric 3D reconstruction using mobile imaging

    Science.gov (United States)

    Fritsch, Dieter; Syll, Miguel

    2015-03-01

    In our paper we demonstrate the development of an Android Application (AndroidSfM) for photogrammetric 3D reconstruction that works on smartphones and tablets likewise. The photos are taken with mobile devices, and can thereafter directly be calibrated using standard calibration algorithms of photogrammetry and computer vision, on that device. Due to still limited computing resources on mobile devices, a client-server handshake using Dropbox transfers the photos to the sever to run AndroidSfM for the pose estimation of all photos by Structure-from-Motion and, thereafter, uses the oriented bunch of photos for dense point cloud estimation by dense image matching algorithms. The result is transferred back to the mobile device for visualization and ad-hoc on-screen measurements.

  12. Tomographic spectral imaging: microanalysis in 3D

    International Nuclear Information System (INIS)

    Kotula, P.G.; Keenan, M.R.; Michael, J.R.

    2003-01-01

    Full text: Spectral imaging, where a series of complete x-ray spectra are typically collected from a 2D area, holds great promise for comprehensive near-surface microanalysis. There are however numerous microanalysis problems where 3D chemical information is needed as well. In the SEM, some sort of sectioning (either mechanical or with a focused ion beam (FIB) tool) followed by x-ray mapping has, in the past, been utilized in an attempt to perform 3D microanalysis. Reliance on simple mapping has the potential to miss important chemical features as well as misidentify others. In this paper we will describe the acquisition of serial-section tomographic spectral images (TSI) with a dual-beam FIB/SEM equipped with an EDS system. We will also describe the application of a modified version of our multivariate statistical analysis algorithms to TSIs. Serial sectioning was performed with a FEI DB-235 FIB/SEM. Firstly, the specimen normal was tilted to the optic axis of the FIB column and a trench was milled into the surface of the specimen. A second trench was then milled perpendicular to the first to provide visibility of the entire analysis surface to the x-ray detector. In addition, several fiducial markers were milled into the surface to allow for alignment from slice to slice. The electron column is at an angle of 52 deg to the ion column so the electron beam can 'see' the analysis surface milled by the FIB with no additional specimen tilting or rotation. Likewise the x-ray detector is at a radial angle of 45 deg to the plane of the electron and ion columns (about the electron column) and a take-off-angle of 35 deg with respect to an untilted specimen so it can 'see' the analysis surface as well with no additional sample tilting or rotation. Spectral images were acquired from regions 40 μm wide and 20μm deep for each slice. Approximately 1μm/slice was milled and 10-12 total slices were cut. Spectral images were acquired with a Thermo NORAN Vantage (Digital imaging

  13. Multiphoton fluorescence lifetime imaging of chemotherapy distribution in solid tumors

    Science.gov (United States)

    Carlson, Marjorie; Watson, Adrienne L.; Anderson, Leah; Largaespada, David A.; Provenzano, Paolo P.

    2017-11-01

    Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, while doxorubicin fluorescence and lifetime have been imaged in live tissue, its behavior in archival samples that frequently result from drug and treatment studies in human and animal patients, and murine models of human cancer, has to date been largely unexplored. Here, we demonstrate imaging of doxorubicin intensity and lifetimes in archival formalin-fixed paraffin-embedded sections from mouse models of human cancer with multiphoton excitation and multiphoton fluorescence lifetime imaging microscopy (FLIM). Multiphoton excitation imaging reveals robust doxorubicin emission in tissue sections and captures spatial heterogeneity in cells and tissues. However, quantifying the amount of doxorubicin signal in distinct cell compartments, particularly the nucleus, often remains challenging due to strong signals in multiple compartments. The addition of FLIM analysis to display the spatial distribution of excited state lifetimes clearly distinguishes between signals in distinct compartments such as the cell nuclei versus cytoplasm and allows for quantification of doxorubicin signal in each compartment. Furthermore, we observed a shift in lifetime values in the nuclei of transformed cells versus nontransformed cells, suggesting a possible diagnostic role for doxorubicin lifetime imaging to distinguish normal versus transformed cells. Thus, data here demonstrate that multiphoton FLIM is a highly sensitive platform for imaging doxorubicin distribution in normal and diseased archival tissues.

  14. Brain morphology imaging by 3D microscopy and fluorescent Nissl staining.

    Science.gov (United States)

    Lazutkin, A A; Komissarova, N V; Toptunov, D M; Anokhin, K V

    2013-07-01

    Modern optical methods (multiphoton and light-sheet fluorescent microscopy) allow 3D imaging of large specimens of the brain with cell resolution. It is therefore essential to refer the resultant 3D pictures of expression of transgene, protein, and other markers in the brain to the corresponding structures in the atlas. This implies counterstaining of specimens with morphological dyes. However, there are no methods for contrasting large samples of the brain without their preliminary slicing. We have developed a method for fluorescent Nissl staining of whole brain samples. 3D reconstructions of specimens of the hippocampus, olfactory bulbs, and cortex were created. The method can be used for morphological control and evaluation of the effects of various factors on the brain using 3D microscopy technique.

  15. 3D object-oriented image analysis in 3D geophysical modelling

    DEFF Research Database (Denmark)

    Fadel, I.; van der Meijde, M.; Kerle, N.

    2015-01-01

    Non-uniqueness of satellite gravity interpretation has traditionally been reduced by using a priori information from seismic tomography models. This reduction in the non-uniqueness has been based on velocity-density conversion formulas or user interpretation of the 3D subsurface structures (objects......) based on the seismic tomography models and then forward modelling these objects. However, this form of object-based approach has been done without a standardized methodology on how to extract the subsurface structures from the 3D models. In this research, a 3D object-oriented image analysis (3D OOA......) approach was implemented to extract the 3D subsurface structures from geophysical data. The approach was applied on a 3D shear wave seismic tomography model of the central part of the East African Rift System. Subsequently, the extracted 3D objects from the tomography model were reconstructed in the 3D...

  16. 3D Printer Generated Tissue iMolds for Cleared Tissue Using Single- and Multi-Photon Microscopy for Deep Tissue Evaluation.

    Science.gov (United States)

    Miller, Sean J; Rothstein, Jeffrey D

    2017-01-01

    Pathological analyses and methodology has recently undergone a dramatic revolution. With the creation of tissue clearing methods such as CLARITY and CUBIC, groups can now achieve complete transparency in tissue samples in nano-porous hydrogels. Cleared tissue is then imagined in a semi-aqueous medium that matches the refractive index of the objective being used. However, one major challenge is the ability to control tissue movement during imaging and to relocate precise locations post sequential clearing and re-staining. Using 3D printers, we designed tissue molds that fit precisely around the specimen being imaged. First, images are taken of the specimen, followed by importing and design of a structural mold, then printed with affordable plastics by a 3D printer. With our novel design, we have innovated tissue molds called innovative molds (iMolds) that can be generated in any laboratory and are customized for any organ, tissue, or bone matter being imaged. Furthermore, the inexpensive and reusable tissue molds are made compatible for any microscope such as single and multi-photon confocal with varying stage dimensions. Excitingly, iMolds can also be generated to hold multiple organs in one mold, making reconstruction and imaging much easier. Taken together, with iMolds it is now possible to image cleared tissue in clearing medium while limiting movement and being able to relocate precise anatomical and cellular locations on sequential imaging events in any basic laboratory. This system provides great potential for screening widespread effects of therapeutics and disease across entire organ systems.

  17. Three-dimensional spatial imaging in multiphoton ionization rate measurements

    International Nuclear Information System (INIS)

    Bredy, Richard; Camp, Howard A.; Nguyen, Hai; Awata, Takaaki; Shan Bing; Chang Zhenghu; DePaola, B.D.

    2004-01-01

    An experiment is described in which an apparatus is used to demonstrate the feasibility of measuring multiphoton photoionization rates in the interaction of short pulsed lasers with atoms or molecules. With this methodology, the ionization rate is measured as a function of the spatial position in the beam-waist region of the laser through the direct three-dimensional spatial imaging of the ionization events. Thus, if the spatial dependence of the laser beam intensity were known, a series of experiments could yield the intensity dependence of multiphoton ionization without the assumptions or errors that are generally inherent in the integration over one or more dimensions in the laser focal volume

  18. Handbook of 3D machine vision optical metrology and imaging

    CERN Document Server

    Zhang, Song

    2013-01-01

    With the ongoing release of 3D movies and the emergence of 3D TVs, 3D imaging technologies have penetrated our daily lives. Yet choosing from the numerous 3D vision methods available can be frustrating for scientists and engineers, especially without a comprehensive resource to consult. Filling this gap, Handbook of 3D Machine Vision: Optical Metrology and Imaging gives an extensive, in-depth look at the most popular 3D imaging techniques. It focuses on noninvasive, noncontact optical methods (optical metrology and imaging). The handbook begins with the well-studied method of stereo vision and

  19. In vivo multiphoton imaging of human skin: assessment of topical corticosteroid-induced epidermis atrophy and depigmentation

    Science.gov (United States)

    Ait El Madani, Hassan; Tancrède-Bohin, Emmanuelle; Bensussan, Armand; Colonna, Anne; Dupuy, Alain; Bagot, Martine; Pena, Ana-Maria

    2012-02-01

    Multiphoton microscopy has emerged in the past decade as a promising tool for noninvasive skin imaging. Our aim was to evaluate the potential of multiphoton microscopy to detect topical corticosteroids side effects within the epidermis and to provide new insights into their dynamics. Healthy volunteers were topically treated with clobetasol propionate on a small region of their forearms under overnight occlusion for three weeks. The treated region of each patient was investigated at D0, D7, D15, D22 (end of the treatment), and D60. Our study shows that multiphoton microscopy allows for the detection of corticoid-induced epidermis modifications: thinning of stratum corneum compactum and epidermis, decrease of keratinocytes size, and changes in their morphology from D7 to D22. We also show that multiphoton microscopy enables in vivo three-dimensional (3-D) quantitative assessment of melanin content. We observe that melanin density decreases during treatment and almost completely disappears at D22. Moreover, these alterations are reversible as they are no longer present at D60. Our study demonstrates that multiphoton microscopy is a convenient and powerful tool for noninvasive 3-D dynamical studies of skin integrity and pigmentation.

  20. Minimally invasive vascular imaging using 3D-CTA and 3D-MRA. Update

    International Nuclear Information System (INIS)

    Hayashi, Hiromitsu; Kawamata, Hiroshi; Takagi, Ryo; Amano, Yasuo; Wakabayashi, Hiroyuki; Ichikawa, Kazuo; Kumazaki, Tatsuo

    1998-01-01

    Conventional angiography is considered the standard of reference for diagnostic imaging of vascular diseases with respect to its temporal and spatial resolution. This procedure, however is invasive and repeated studies are difficult, and arterial complications are occasionally associated in catheter-based conventional angiography. Recent advances in diagnostic imaging have facilitated three-dimensional CT angiography (3D-CTA) using the volumetric acquisition capabilities inherent in spiral CT and three-dimensional MR angiography (3D-MRA) using the 3D gradient-echo sequence with a bolus injection of Gd-DTPA. These techniques can provide vascular images exceedingly similar to conventional angiograms within a short acquisition time. 3D-CTA and 3D-MRA are considered to be promising, minimally invasive methods for obtaining images of the vasculature, and alternatives to catheter angiography. This study reviews the current status of 3D-CTA and 3D-MRA, with emphasis on the clinical usefulness of three-dimensional diagnostic imaging for the evaluation of diverse vascular pathologies. (author)

  1. 3D widefield light microscope image reconstruction without dyes

    Science.gov (United States)

    Larkin, S.; Larson, J.; Holmes, C.; Vaicik, M.; Turturro, M.; Jurkevich, A.; Sinha, S.; Ezashi, T.; Papavasiliou, G.; Brey, E.; Holmes, T.

    2015-03-01

    3D image reconstruction using light microscope modalities without exogenous contrast agents is proposed and investigated as an approach to produce 3D images of biological samples for live imaging applications. Multimodality and multispectral imaging, used in concert with this 3D optical sectioning approach is also proposed as a way to further produce contrast that could be specific to components in the sample. The methods avoid usage of contrast agents. Contrast agents, such as fluorescent or absorbing dyes, can be toxic to cells or alter cell behavior. Current modes of producing 3D image sets from a light microscope, such as 3D deconvolution algorithms and confocal microscopy generally require contrast agents. Zernike phase contrast (ZPC), transmitted light brightfield (TLB), darkfield microscopy and others can produce contrast without dyes. Some of these modalities have not previously benefitted from 3D image reconstruction algorithms, however. The 3D image reconstruction algorithm is based on an underlying physical model of scattering potential, expressed as the sample's 3D absorption and phase quantities. The algorithm is based upon optimizing an objective function - the I-divergence - while solving for the 3D absorption and phase quantities. Unlike typical deconvolution algorithms, each microscope modality, such as ZPC or TLB, produces two output image sets instead of one. Contrast in the displayed image and 3D renderings is further enabled by treating the multispectral/multimodal data as a feature set in a mathematical formulation that uses the principal component method of statistics.

  2. 3D ultrasound imaging for prosthesis fabrication and diagnostic imaging

    Energy Technology Data Exchange (ETDEWEB)

    Morimoto, A.K.; Bow, W.J.; Strong, D.S. [and others

    1995-06-01

    The fabrication of a prosthetic socket for a below-the-knee amputee requires knowledge of the underlying bone structure in order to provide pressure relief for sensitive areas and support for load bearing areas. The goal is to enable the residual limb to bear pressure with greater ease and utility. Conventional methods of prosthesis fabrication are based on limited knowledge about the patient`s underlying bone structure. A 3D ultrasound imaging system was developed at Sandia National Laboratories. The imaging system provides information about the location of the bones in the residual limb along with the shape of the skin surface. Computer assisted design (CAD) software can use this data to design prosthetic sockets for amputees. Ultrasound was selected as the imaging modality. A computer model was developed to analyze the effect of the various scanning parameters and to assist in the design of the overall system. The 3D ultrasound imaging system combines off-the-shelf technology for image capturing, custom hardware, and control and image processing software to generate two types of image data -- volumetric and planar. Both volumetric and planar images reveal definition of skin and bone geometry with planar images providing details on muscle fascial planes, muscle/fat interfaces, and blood vessel definition. The 3D ultrasound imaging system was tested on 9 unilateral below-the- knee amputees. Image data was acquired from both the sound limb and the residual limb. The imaging system was operated in both volumetric and planar formats. An x-ray CT (Computed Tomography) scan was performed on each amputee for comparison. Results of the test indicate beneficial use of ultrasound to generate databases for fabrication of prostheses at a lower cost and with better initial fit as compared to manually fabricated prostheses.

  3. Measurable realistic image-based 3D mapping

    Science.gov (United States)

    Liu, W.; Wang, J.; Wang, J. J.; Ding, W.; Almagbile, A.

    2011-12-01

    Maps with 3D visual models are becoming a remarkable feature of 3D map services. High-resolution image data is obtained for the construction of 3D visualized models.The3D map not only provides the capabilities of 3D measurements and knowledge mining, but also provides the virtual experienceof places of interest, such as demonstrated in the Google Earth. Applications of 3D maps are expanding into the areas of architecture, property management, and urban environment monitoring. However, the reconstruction of high quality 3D models is time consuming, and requires robust hardware and powerful software to handle the enormous amount of data. This is especially for automatic implementation of 3D models and the representation of complicated surfacesthat still need improvements with in the visualisation techniques. The shortcoming of 3D model-based maps is the limitation of detailed coverage since a user can only view and measure objects that are already modelled in the virtual environment. This paper proposes and demonstrates a 3D map concept that is realistic and image-based, that enables geometric measurements and geo-location services. Additionally, image-based 3D maps provide more detailed information of the real world than 3D model-based maps. The image-based 3D maps use geo-referenced stereo images or panoramic images. The geometric relationships between objects in the images can be resolved from the geometric model of stereo images. The panoramic function makes 3D maps more interactive with users but also creates an interesting immersive circumstance. Actually, unmeasurable image-based 3D maps already exist, such as Google street view, but only provide virtual experiences in terms of photos. The topographic and terrain attributes, such as shapes and heights though are omitted. This paper also discusses the potential for using a low cost land Mobile Mapping System (MMS) to implement realistic image 3D mapping, and evaluates the positioning accuracy that a measureable

  4. Micromachined Ultrasonic Transducers for 3-D Imaging

    DEFF Research Database (Denmark)

    Christiansen, Thomas Lehrmann

    of state-of-the-art 3-D ultrasound systems. The focus is on row-column addressed transducer arrays. This previously sparsely investigated addressing scheme offers a highly reduced number of transducer elements, resulting in reduced transducer manufacturing costs and data processing. To produce...... such transducer arrays, capacitive micromachined ultrasonic transducer (CMUT) technology is chosen for this project. Properties such as high bandwidth and high design flexibility makes this an attractive transducer technology, which is under continuous development in the research community. A theoretical...... treatment of CMUTs is presented, including investigations of the anisotropic plate behaviour and modal radiation patterns of such devices. Several new CMUT fabrication approaches are developed and investigated in terms of oxide quality and surface protrusions, culminating in a simple four-mask process...

  5. 3D Imaging with Structured Illumination for Advanced Security Applications

    Energy Technology Data Exchange (ETDEWEB)

    Birch, Gabriel Carisle [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Dagel, Amber Lynn [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Kast, Brian A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Smith, Collin S. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-09-01

    Three-dimensional (3D) information in a physical security system is a highly useful dis- criminator. The two-dimensional data from an imaging systems fails to provide target dis- tance and three-dimensional motion vector, which can be used to reduce nuisance alarm rates and increase system effectiveness. However, 3D imaging devices designed primarily for use in physical security systems are uncommon. This report discusses an architecture favorable to physical security systems; an inexpensive snapshot 3D imaging system utilizing a simple illumination system. The method of acquiring 3D data, tests to understand illumination de- sign, and software modifications possible to maximize information gathering capability are discussed.

  6. On Alternative Approaches to 3D Image Perception: Monoscopic 3D Techniques

    Science.gov (United States)

    Blundell, Barry G.

    2015-06-01

    In the eighteenth century, techniques that enabled a strong sense of 3D perception to be experienced without recourse to binocular disparities (arising from the spatial separation of the eyes) underpinned the first significant commercial sales of 3D viewing devices and associated content. However following the advent of stereoscopic techniques in the nineteenth century, 3D image depiction has become inextricably linked to binocular parallax and outside the vision science and arts communities relatively little attention has been directed towards earlier approaches. Here we introduce relevant concepts and terminology and consider a number of techniques and optical devices that enable 3D perception to be experienced on the basis of planar images rendered from a single vantage point. Subsequently we allude to possible mechanisms for non-binocular parallax based 3D perception. Particular attention is given to reviewing areas likely to be thought-provoking to those involved in 3D display development, spatial visualization, HCI, and other related areas of interdisciplinary research.

  7. Developing 3D Imaging Programmes-Workflow and Quality Control

    OpenAIRE

    Hess, M.; Robson, S.; Serpico, M.; Amati, G.; Pridden, I.; Nelson, T.

    2016-01-01

    This article reports on a successful project for 3D imaging research, digital applications, and use of new technologies in the museum. The article will focus on the development and implementation of a viable workflow for the production of high-quality 3D models of museum objects, based on the 3D laser scanning and photogrammetry of selected ancient Egyptian artefacts. The development of a robust protocol for the complete process chain for imaging cultural heritage artefacts, from the acquisit...

  8. Development of 3-D Medical Image VIsualization System

    African Journals Online (AJOL)

    User

    uses standard 2-D medical imaging inputs and generates medical images of human body parts ... light wave from points on the 3-D object(s) in ... tools, and communication bandwidth cannot .... locations along the track that correspond with.

  9. Denoising imaging polarimetry by adapted BM3D method.

    Science.gov (United States)

    Tibbs, Alexander B; Daly, Ilse M; Roberts, Nicholas W; Bull, David R

    2018-04-01

    In addition to the visual information contained in intensity and color, imaging polarimetry allows visual information to be extracted from the polarization of light. However, a major challenge of imaging polarimetry is image degradation due to noise. This paper investigates the mitigation of noise through denoising algorithms and compares existing denoising algorithms with a new method, based on BM3D (Block Matching 3D). This algorithm, Polarization-BM3D (PBM3D), gives visual quality superior to the state of the art across all images and noise standard deviations tested. We show that denoising polarization images using PBM3D allows the degree of polarization to be more accurately calculated by comparing it with spectral polarimetry measurements.

  10. 3D Reconstruction of NMR Images by LabVIEW

    Directory of Open Access Journals (Sweden)

    Peter IZAK

    2007-01-01

    Full Text Available This paper introduces the experiment of 3D reconstruction NMR images via virtual instrumentation - LabVIEW. The main idea is based on marching cubes algorithm and image processing implemented by module of Vision assistant. The two dimensional images shot by the magnetic resonance device provide information about the surface properties of human body. There is implemented algorithm which can be used for 3D reconstruction of magnetic resonance images in biomedical application.

  11. Microstructure imaging of human rectal mucosa using multiphoton microscopy

    Science.gov (United States)

    Liu, N. R.; Chen, G.; Chen, J. X.; Yan, J.; Zhuo, S. M.; Zheng, L. Q.; Jiang, X. S.

    2011-01-01

    Multiphoton microscopy (MPM) has high resolution and sensitivity. In this study, MPM was used to image microstructure of human rectal mucosa. The morphology and distribution of the main components in mucosa layer, absorptive cells and goblet cells in the epithelium, abundant intestinal glands in the lamina propria and smooth muscle fibers in the muscularis mucosa were clearly monitored. The variations of these components were tightly relevant to the pathology in gastrointestine system, especially early rectal cancer. The obtained images will be helpful for the diagnosis of early colorectal cancer.

  12. 3D Interpolation Method for CT Images of the Lung

    Directory of Open Access Journals (Sweden)

    Noriaki Asada

    2003-06-01

    Full Text Available A 3-D image can be reconstructed from numerous CT images of the lung. The procedure reconstructs a solid from multiple cross section images, which are collected during pulsation of the heart. Thus the motion of the heart is a special factor that must be taken into consideration during reconstruction. The lung exhibits a repeating transformation synchronized to the beating of the heart as an elastic body. There are discontinuities among neighboring CT images due to the beating of the heart, if no special techniques are used in taking CT images. The 3-D heart image is reconstructed from numerous CT images in which both the heart and the lung are taken. Although the outline shape of the reconstructed 3-D heart is quite unnatural, the envelope of the 3-D unnatural heart is fit to the shape of the standard heart. The envelopes of the lung in the CT images are calculated after the section images of the best fitting standard heart are located at the same positions of the CT images. Thus the CT images are geometrically transformed to the optimal CT images fitting best to the standard heart. Since correct transformation of images is required, an Area oriented interpolation method proposed by us is used for interpolation of transformed images. An attempt to reconstruct a 3-D lung image by a series of such operations without discontinuity is shown. Additionally, the same geometrical transformation method to the original projection images is proposed as a more advanced method.

  13. Novel Organic Materials for Multi-photon Photopolymerization and Photografting: Powerful Tools for Precise Microfabracation and Functionalization in 3D

    Energy Technology Data Exchange (ETDEWEB)

    Li, Z.

    2013-07-01

    benzylidene ketone-based 2PIs containing double bonds and dialkylamino groups were synthesized in one step via classical aldol condensation reactions. The results of quantum-chemical calculations and experimental tests indicated that the size of the central ring significantly affected the excited state energetics and emission quantum yields as well as the two-photon initiation efficiency. 4-methylcyclohexanone-based initiator M2CMK is far more efficient than its counterparts with a central five-membered ring. The ideal processing windows of M2CMK are as broad as those of B3FL but with much simpler synthesis. Straightforward synthesis combined with high 2PA initiation efficiency makes the novel initiator a promising candidate for commercialization. Based on the efficient core structures of cyclic benzylidene ketone, carboxylic acid sodium salts as water-borne functionalities were incorporated in order to expand the application range of 2PP to biofabrication. Those novel water-soluble 2PIs were applied to microfabrication at a writing speed as high as 100 mm/s within hydrophilic photopolymers with up to 50 wt% of water. Preliminary dark-cytotoxicity tests of the 2PIs were performed and the obtained results were compared to those of Irgacure 2959, the most commonly used photoinitiator in cell encapsulation. The second part of the thesis concerned on the novel arylazide used for multi-photon induced photografting (MPG), a powerful tool for 3D site-specific functionalization. As a proof-of-concept, commercial aromatic azide BAC-M was successfully grafted within the 3D matrix with high resolution under three-photon excitation. In order to enhance the grafting efficiency and reduce the required energies, we designed and synthesized a series of novel 2PA active fluoroaryl azides containing 'push-pull' structures. Desired functionalities, such as alkene and alkyne groups, were introduced at the terminal amino groups for post-modification after two-photon photografting. (author

  14. 3D integration technologies for imaging applications

    International Nuclear Information System (INIS)

    Moor, Piet de

    2008-01-01

    The aim of this paper is to give an overview of micro-electronic technologies under development today, and how they are impacting on the radiation detection and imaging of tomorrow. After a short introduction, the different enabling technologies will be discussed. Finally, a few examples of ongoing developments at IMEC on advanced detector systems will be given

  15. Design, Fabrication and Computational Characterization of a 3D Micro-Valve Built by Multi-Photon Polymerization

    Directory of Open Access Journals (Sweden)

    Stratos Galanopoulos

    2014-08-01

    Full Text Available We report on the design, modeling and fabrication by multi-photon polymerization of a complex medical fluidic device. The physical dimensions of the built micro-valve prototype are compared to those of its computer-designed model. Important fabrication issues such as achieving high dimensional resolution and ability to control distortion due to shrinkage are presented and discussed. The operational performance of both multi-photon and CAD-created models under steady blood flow conditions was evaluated and compared through computational fluid dynamics analysis.

  16. Acoustic 3D imaging of dental structures

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, D.K. [Lawrence Livermore National Lab., CA (United States); Hume, W.R. [California Univ., Los Angeles, CA (United States); Douglass, G.D. [California Univ., San Francisco, CA (United States)

    1997-02-01

    Our goals for the first year of this three dimensional electodynamic imaging project was to determine how to combine flexible, individual addressable; preprocessing of array source signals; spectral extrapolation or received signals; acoustic tomography codes; and acoustic propagation modeling code. We investigated flexible, individually addressable acoustic array material to find the best match in power, sensitivity and cost and settled on PVDF sheet arrays and 3-1 composite material.

  17. In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue.

    Science.gov (United States)

    Kantelhardt, Sven R; Kalasauskas, Darius; König, Karsten; Kim, Ella; Weinigel, Martin; Uchugonova, Aisada; Giese, Alf

    2016-05-01

    High resolution multiphoton tomography and fluorescence lifetime imaging differentiates glioma from adjacent brain in native tissue samples ex vivo. Presently, multiphoton tomography is applied in clinical dermatology and experimentally. We here present the first application of multiphoton and fluorescence lifetime imaging for in vivo imaging on humans during a neurosurgical procedure. We used a MPTflex™ Multiphoton Laser Tomograph (JenLab, Germany). We examined cultured glioma cells in an orthotopic mouse tumor model and native human tissue samples. Finally the multiphoton tomograph was applied to provide optical biopsies during resection of a clinical case of glioblastoma. All tissues imaged by multiphoton tomography were sampled and processed for conventional histopathology. The multiphoton tomograph allowed fluorescence intensity- and fluorescence lifetime imaging with submicron spatial resolution and 200 picosecond temporal resolution. Morphological fluorescence intensity imaging and fluorescence lifetime imaging of tumor-bearing mouse brains and native human tissue samples clearly differentiated tumor and adjacent brain tissue. Intraoperative imaging was found to be technically feasible. Intraoperative image quality was comparable to ex vivo examinations. To our knowledge we here present the first intraoperative application of high resolution multiphoton tomography and fluorescence lifetime imaging of human brain tumors in situ. It allowed in vivo identification and determination of cell density of tumor tissue on a cellular and subcellular level within seconds. The technology shows the potential of rapid intraoperative identification of native glioma tissue without need for tissue processing or staining.

  18. 3D surface reconstruction using optical flow for medical imaging

    International Nuclear Information System (INIS)

    Weng, Nan; Yang, Yee-Hong; Pierson, R.

    1996-01-01

    The recovery of a 3D model from a sequence of 2D images is very useful in medical image analysis. Image sequences obtained from the relative motion between the object and the camera or the scanner contain more 3D information than a single image. Methods to visualize the computed tomograms can be divided into two approaches: the surface rendering approach and the volume rendering approach. A new surface rendering method using optical flow is proposed. Optical flow is the apparent motion in the image plane produced by the projection of the real 3D motion onto 2D image. In this paper, the object remains stationary while the scanner undergoes translational motion. The 3D motion of an object can be recovered from the optical flow field using additional constraints. By extracting the surface information from 3D motion, it is possible to get an accurate 3D model of the object. Both synthetic and real image sequences have been used to illustrate the feasibility of the proposed method. The experimental results suggest that the proposed method is suitable for the reconstruction of 3D models from ultrasound medical images as well as other computed tomograms

  19. Diffractive optical element for creating visual 3D images.

    Science.gov (United States)

    Goncharsky, Alexander; Goncharsky, Anton; Durlevich, Svyatoslav

    2016-05-02

    A method is proposed to compute and synthesize the microrelief of a diffractive optical element to produce a new visual security feature - the vertical 3D/3D switch effect. The security feature consists in the alternation of two 3D color images when the diffractive element is tilted up/down. Optical security elements that produce the new security feature are synthesized using electron-beam technology. Sample optical security elements are manufactured that produce 3D to 3D visual switch effect when illuminated by white light. Photos and video records of the vertical 3D/3D switch effect of real optical elements are presented. The optical elements developed can be replicated using standard equipment employed for manufacturing security holograms. The new optical security feature is easy to control visually, safely protected against counterfeit, and designed to protect banknotes, documents, ID cards, etc.

  20. 3D/3D registration of coronary CTA and biplane XA reconstructions for improved image guidance

    Energy Technology Data Exchange (ETDEWEB)

    Dibildox, Gerardo, E-mail: g.dibildox@erasmusmc.nl; Baka, Nora; Walsum, Theo van [Biomedical Imaging Group Rotterdam, Departments of Radiology and Medical Informatics, Erasmus Medical Center, 3015 GE Rotterdam (Netherlands); Punt, Mark; Aben, Jean-Paul [Pie Medical Imaging, 6227 AJ Maastricht (Netherlands); Schultz, Carl [Department of Cardiology, Erasmus Medical Center, 3015 GE Rotterdam (Netherlands); Niessen, Wiro [Quantitative Imaging Group, Faculty of Applied Sciences, Delft University of Technology, 2628 CJ Delft, The Netherlands and Biomedical Imaging Group Rotterdam, Departments of Radiology and Medical Informatics, Erasmus Medical Center, 3015 GE Rotterdam (Netherlands)

    2014-09-15

    Purpose: The authors aim to improve image guidance during percutaneous coronary interventions of chronic total occlusions (CTO) by providing information obtained from computed tomography angiography (CTA) to the cardiac interventionist. To this end, the authors investigate a method to register a 3D CTA model to biplane reconstructions. Methods: The authors developed a method for registering preoperative coronary CTA with intraoperative biplane x-ray angiography (XA) images via 3D models of the coronary arteries. The models are extracted from the CTA and biplane XA images, and are temporally aligned based on CTA reconstruction phase and XA ECG signals. Rigid spatial alignment is achieved with a robust probabilistic point set registration approach using Gaussian mixture models (GMMs). This approach is extended by including orientation in the Gaussian mixtures and by weighting bifurcation points. The method is evaluated on retrospectively acquired coronary CTA datasets of 23 CTO patients for which biplane XA images are available. Results: The Gaussian mixture model approach achieved a median registration accuracy of 1.7 mm. The extended GMM approach including orientation was not significantly different (P > 0.1) but did improve robustness with regards to the initialization of the 3D models. Conclusions: The authors demonstrated that the GMM approach can effectively be applied to register CTA to biplane XA images for the purpose of improving image guidance in percutaneous coronary interventions.

  1. 3-D Reconstruction From Satellite Images

    DEFF Research Database (Denmark)

    Denver, Troelz

    1999-01-01

    of planetary surfaces, but other purposes is considered as well. The system performance is measured with respect to the precision and the time consumption.The reconstruction process is divided into four major areas: Acquisition, calibration, matching/reconstruction and presentation. Each of these areas...... are treated individually. A detailed treatment of various lens distortions is required, in order to correct for these problems. This subject is included in the acquisition part. In the calibration part, the perspective distortion is removed from the images. Most attention has been paid to the matching problem...

  2. 3D-LSI technology for image sensor

    International Nuclear Information System (INIS)

    Motoyoshi, Makoto; Koyanagi, Mitsumasa

    2009-01-01

    Recently, the development of three-dimensional large-scale integration (3D-LSI) technologies has accelerated and has advanced from the research level or the limited production level to the investigation level, which might lead to mass production. By separating 3D-LSI technology into elementary technologies such as (1) through silicon via (TSV) formation, (2) bump formation, (3) wafer thinning, (4) chip/wafer alignment, and (5) chip/wafer stacking and reconstructing the entire process and structure, many methods to realize 3D-LSI devices can be developed. However, by considering a specific application, the supply chain of base wafers, and the purpose of 3D integration, a few suitable combinations can be identified. In this paper, we focus on the application of 3D-LSI technologies to image sensors. We describe the process and structure of the chip size package (CSP), developed on the basis of current and advanced 3D-LSI technologies, to be used in CMOS image sensors. Using the current LSI technologies, CSPs for 1.3 M, 2 M, and 5 M pixel CMOS image sensors were successfully fabricated without any performance degradation. 3D-LSI devices can be potentially employed in high-performance focal-plane-array image sensors. We propose a high-speed image sensor with an optical fill factor of 100% to be developed using next-generation 3D-LSI technology and fabricated using micro(μ)-bumps and micro(μ)-TSVs.

  3. Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization

    Science.gov (United States)

    2014-05-01

    1 Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization David N. Ford...2014 4. TITLE AND SUBTITLE Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization 5a...Manufacturing ( 3D printing ) 2 Research Context Problem: Learning curve savings forecasted in SHIPMAIN maintenance initiative have not materialized

  4. In vivo multiphoton imaging of bile duct ligation

    Science.gov (United States)

    Liu, Yuan; Li, Feng-Chieh; Chen, Hsiao-Chin; Chang, Po-shou; Yang, Shu-Mei; Lee, Hsuan-Shu; Dong, Chen-Yuan

    2008-02-01

    Bile is the exocrine secretion of liver and synthesized by hepatocytes. It is drained into duodenum for the function of digestion or drained into gallbladder for of storage. Bile duct obstruction is a blockage in the tubes that carry bile to the gallbladder and small intestine. However, Bile duct ligation results in the changes of bile acids in serum, liver, urine, and feces1, 2. In this work, we demonstrate a novel technique to image this pathological condition by using a newly developed in vivo imaging system, which includes multiphoton microscopy and intravital hepatic imaging chamber. The images we acquired demonstrate the uptake, processing of 6-CFDA in hepatocytes and excretion of CF in the bile canaliculi. In addition to imaging, we can also measure kinetics of the green fluorescence intensity.

  5. 3D reconstruction based on light field images

    Science.gov (United States)

    Zhu, Dong; Wu, Chunhong; Liu, Yunluo; Fu, Dongmei

    2018-04-01

    This paper proposed a method of reconstructing three-dimensional (3D) scene from two light field images capture by Lytro illium. The work was carried out by first extracting the sub-aperture images from light field images and using the scale-invariant feature transform (SIFT) for feature registration on the selected sub-aperture images. Structure from motion (SFM) algorithm is further used on the registration completed sub-aperture images to reconstruct the three-dimensional scene. 3D sparse point cloud was obtained in the end. The method shows that the 3D reconstruction can be implemented by only two light field camera captures, rather than at least a dozen times captures by traditional cameras. This can effectively solve the time-consuming, laborious issues for 3D reconstruction based on traditional digital cameras, to achieve a more rapid, convenient and accurate reconstruction.

  6. Deep learning for objective quality assessment of 3D images

    NARCIS (Netherlands)

    Mocanu, D.C.; Exarchakos, G.; Liotta, A.

    2014-01-01

    Improving the users' Quality of Experience (QoE) in modern 3D Multimedia Systems is a challenging proposition, mainly due to our limited knowledge of 3D image Quality Assessment algorithms. While subjective QoE methods would better reflect the nature of human perception, these are not suitable in

  7. 3D quantitative phase imaging of neural networks using WDT

    Science.gov (United States)

    Kim, Taewoo; Liu, S. C.; Iyer, Raj; Gillette, Martha U.; Popescu, Gabriel

    2015-03-01

    White-light diffraction tomography (WDT) is a recently developed 3D imaging technique based on a quantitative phase imaging system called spatial light interference microscopy (SLIM). The technique has achieved a sub-micron resolution in all three directions with high sensitivity granted by the low-coherence of a white-light source. Demonstrations of the technique on single cell imaging have been presented previously; however, imaging on any larger sample, including a cluster of cells, has not been demonstrated using the technique. Neurons in an animal body form a highly complex and spatially organized 3D structure, which can be characterized by neuronal networks or circuits. Currently, the most common method of studying the 3D structure of neuron networks is by using a confocal fluorescence microscope, which requires fluorescence tagging with either transient membrane dyes or after fixation of the cells. Therefore, studies on neurons are often limited to samples that are chemically treated and/or dead. WDT presents a solution for imaging live neuron networks with a high spatial and temporal resolution, because it is a 3D imaging method that is label-free and non-invasive. Using this method, a mouse or rat hippocampal neuron culture and a mouse dorsal root ganglion (DRG) neuron culture have been imaged in order to see the extension of processes between the cells in 3D. Furthermore, the tomogram is compared with a confocal fluorescence image in order to investigate the 3D structure at synapses.

  8. Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points

    Science.gov (United States)

    Karreman, Matthia A.; Mercier, Luc; Schieber, Nicole L.; Shibue, Tsukasa; Schwab, Yannick; Goetz, Jacky G.

    2014-01-01

    Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. Here, we investigate correlative methods that rely on the use of artificial and endogenous structural features of the sample as reference points for correlating intravital fluorescence microscopy and electron microscopy. To investigate tumor cell behavior in vivo with ultrastructural accuracy, a reliable approach is needed to retrieve single tumor cells imaged deep within the tissue. For this purpose, fluorescently labeled tumor cells were subcutaneously injected into a mouse ear and imaged using two-photon-excitation microscopy. Using near-infrared branding, the position of the imaged area within the sample was labeled at the skin level, allowing for its precise recollection. Following sample preparation for electron microscopy, concerted usage of the artificial branding and anatomical landmarks enables targeting and approaching the cells of interest while serial sectioning through the specimen. We describe here three procedures showing how three-dimensional (3D) mapping of structural features in the tissue can be exploited to accurately correlate between the two imaging modalities, without having to rely on the use of artificially introduced markers of the region of interest. The methods employed here facilitate the link between intravital and nanoscale imaging of invasive tumor cells, enabling correlating function to structure in the study of tumor invasion and metastasis. PMID:25479106

  9. Preliminary examples of 3D vector flow imaging

    DEFF Research Database (Denmark)

    Pihl, Michael Johannes; Stuart, Matthias Bo; Tomov, Borislav Gueorguiev

    2013-01-01

    This paper presents 3D vector flow images obtained using the 3D Transverse Oscillation (TO) method. The method employs a 2D transducer and estimates the three velocity components simultaneously, which is important for visualizing complex flow patterns. Data are acquired using the experimental ult...... as opposed to magnetic resonance imaging (MRI). The results demonstrate that the 3D TO method is capable of performing 3D vector flow imaging.......This paper presents 3D vector flow images obtained using the 3D Transverse Oscillation (TO) method. The method employs a 2D transducer and estimates the three velocity components simultaneously, which is important for visualizing complex flow patterns. Data are acquired using the experimental...... ultrasound scanner SARUS on a flow rig system with steady flow. The vessel of the flow-rig is centered at a depth of 30 mm, and the flow has an expected 2D circular-symmetric parabolic prole with a peak velocity of 1 m/s. Ten frames of 3D vector flow images are acquired in a cross-sectional plane orthogonal...

  10. Software for 3D diagnostic image reconstruction and analysis

    International Nuclear Information System (INIS)

    Taton, G.; Rokita, E.; Sierzega, M.; Klek, S.; Kulig, J.; Urbanik, A.

    2005-01-01

    Recent advances in computer technologies have opened new frontiers in medical diagnostics. Interesting possibilities are the use of three-dimensional (3D) imaging and the combination of images from different modalities. Software prepared in our laboratories devoted to 3D image reconstruction and analysis from computed tomography and ultrasonography is presented. In developing our software it was assumed that it should be applicable in standard medical practice, i.e. it should work effectively with a PC. An additional feature is the possibility of combining 3D images from different modalities. The reconstruction and data processing can be conducted using a standard PC, so low investment costs result in the introduction of advanced and useful diagnostic possibilities. The program was tested on a PC using DICOM data from computed tomography and TIFF files obtained from a 3D ultrasound system. The results of the anthropomorphic phantom and patient data were taken into consideration. A new approach was used to achieve spatial correlation of two independently obtained 3D images. The method relies on the use of four pairs of markers within the regions under consideration. The user selects the markers manually and the computer calculates the transformations necessary for coupling the images. The main software feature is the possibility of 3D image reconstruction from a series of two-dimensional (2D) images. The reconstructed 3D image can be: (1) viewed with the most popular methods of 3D image viewing, (2) filtered and processed to improve image quality, (3) analyzed quantitatively (geometrical measurements), and (4) coupled with another, independently acquired 3D image. The reconstructed and processed 3D image can be stored at every stage of image processing. The overall software performance was good considering the relatively low costs of the hardware used and the huge data sets processed. The program can be freely used and tested (source code and program available at

  11. Image based 3D city modeling : Comparative study

    Directory of Open Access Journals (Sweden)

    S. P. Singh

    2014-06-01

    Full Text Available 3D city model is a digital representation of the Earth’s surface and it’s related objects such as building, tree, vegetation, and some manmade feature belonging to urban area. The demand of 3D city modeling is increasing rapidly for various engineering and non-engineering applications. Generally four main image based approaches were used for virtual 3D city models generation. In first approach, researchers were used Sketch based modeling, second method is Procedural grammar based modeling, third approach is Close range photogrammetry based modeling and fourth approach is mainly based on Computer Vision techniques. SketchUp, CityEngine, Photomodeler and Agisoft Photoscan are the main softwares to represent these approaches respectively. These softwares have different approaches & methods suitable for image based 3D city modeling. Literature study shows that till date, there is no complete such type of comparative study available to create complete 3D city model by using images. This paper gives a comparative assessment of these four image based 3D modeling approaches. This comparative study is mainly based on data acquisition methods, data processing techniques and output 3D model products. For this research work, study area is the campus of civil engineering department, Indian Institute of Technology, Roorkee (India. This 3D campus acts as a prototype for city. This study also explains various governing parameters, factors and work experiences. This research work also gives a brief introduction, strengths and weakness of these four image based techniques. Some personal comment is also given as what can do or what can’t do from these softwares. At the last, this study shows; it concluded that, each and every software has some advantages and limitations. Choice of software depends on user requirements of 3D project. For normal visualization project, SketchUp software is a good option. For 3D documentation record, Photomodeler gives good

  12. EISCAT Aperture Synthesis Imaging (EASI _3D) for the EISCAT_3D Project

    Science.gov (United States)

    La Hoz, Cesar; Belyey, Vasyl

    2012-07-01

    Aperture Synthesis Imaging Radar (ASIR) is one of the technologies adopted by the EISCAT_3D project to endow it with imaging capabilities in 3-dimensions that includes sub-beam resolution. Complemented by pulse compression, it will provide 3-dimensional images of certain types of incoherent scatter radar targets resolved to about 100 metres at 100 km range, depending on the signal-to-noise ratio. This ability will open new research opportunities to map small structures associated with non-homogeneous, unstable processes such as aurora, summer and winter polar radar echoes (PMSE and PMWE), Natural Enhanced Ion Acoustic Lines (NEIALs), structures excited by HF ionospheric heating, meteors, space debris, and others. The underlying physico-mathematical principles of the technique are the same as the technique employed in radioastronomy to image stellar objects; both require sophisticated inversion techniques to obtain reliable images.

  13. Optical 3D watermark based digital image watermarking for telemedicine

    Science.gov (United States)

    Li, Xiao Wei; Kim, Seok Tae

    2013-12-01

    Region of interest (ROI) of a medical image is an area including important diagnostic information and must be stored without any distortion. This algorithm for application of watermarking technique for non-ROI of the medical image preserving ROI. The paper presents a 3D watermark based medical image watermarking scheme. In this paper, a 3D watermark object is first decomposed into 2D elemental image array (EIA) by a lenslet array, and then the 2D elemental image array data is embedded into the host image. The watermark extraction process is an inverse process of embedding. The extracted EIA through the computational integral imaging reconstruction (CIIR) technique, the 3D watermark can be reconstructed. Because the EIA is composed of a number of elemental images possesses their own perspectives of a 3D watermark object. Even though the embedded watermark data badly damaged, the 3D virtual watermark can be successfully reconstructed. Furthermore, using CAT with various rule number parameters, it is possible to get many channels for embedding. So our method can recover the weak point having only one transform plane in traditional watermarking methods. The effectiveness of the proposed watermarking scheme is demonstrated with the aid of experimental results.

  14. From medical imaging data to 3D printed anatomical models.

    Directory of Open Access Journals (Sweden)

    Thore M Bücking

    Full Text Available Anatomical models are important training and teaching tools in the clinical environment and are routinely used in medical imaging research. Advances in segmentation algorithms and increased availability of three-dimensional (3D printers have made it possible to create cost-efficient patient-specific models without expert knowledge. We introduce a general workflow that can be used to convert volumetric medical imaging data (as generated by Computer Tomography (CT to 3D printed physical models. This process is broken up into three steps: image segmentation, mesh refinement and 3D printing. To lower the barrier to entry and provide the best options when aiming to 3D print an anatomical model from medical images, we provide an overview of relevant free and open-source image segmentation tools as well as 3D printing technologies. We demonstrate the utility of this streamlined workflow by creating models of ribs, liver, and lung using a Fused Deposition Modelling 3D printer.

  15. Advances in polarization sensitive multiphoton nano-bio-imaging

    Directory of Open Access Journals (Sweden)

    Zyss J.

    2010-06-01

    Full Text Available In this talk, we shall shortly review four main directions of ongoing research in our laboratories, directed at the conception and demonstration of a variety of innovative configurations in nanoscale multiphoton imaging. A common feature to all of these directions appears to be the central role played by the involvement of polarization features, both in- and outgoing, moreover so in view of the tensorial aspects inherent to nonlinear schemes such second-harmonic generation, electro-optic modulation or two-photon fluorescence which will ne emphasized. These advances relate to the new domain of nonlinear ellipsometry in multiphoton imaging [1], of high relevance to fundamental aspects of nanophotonics and nanomaterial engineering as well as towards basic life science issues. The four domains to be shortly reported are: a polarization resolved second-harmonic generation in semiconductor QD’s with record small sizes in the 10-12 nm range [2] b original use of two-photon confocal polarization resolved microscopy in DNA stained by two photon fluorescent dyes in different LC phases arrangements so as to characterize these as well as ascertain the respective DNA-dye orientation (intercalant or groves [3] c elaboration and demonstration of an electrooptic confocal microscope in a highly sensitive interferometric and homodyne detection configuration allowing to map weak electric potentials such as in artificial functionalized membranes, the dynamical investigation of firing and propagation aspects of action potentials in neurones being currently the next step [4] d original plasmon based enhanced nanoscale confocal imaging involving a dual detection scheme (fluorescence imaging and ATR plasmon coupling in reflection whereby adequate preparation and switching of the incoming polarization state between radial, linear and azimuthal configurations, entail different images and plasmon enhancement levels [5].

  16. Multiphoton autofluorescence lifetime imaging of induced pluripotent stem cells

    Science.gov (United States)

    Uchugonova, Aisada

    2017-06-01

    The multiphoton fluorescence lifetime imaging tomograph MPTflex with its flexible 360-deg scan head, articulated arm, and tunable femtosecond laser source was employed to study induced pluripotent stem cell (iPS) cultures. Autofluorescence (AF) lifetime imaging was performed with 250-ps temporal resolution and submicron spatial resolution using time-correlated single-photon counting. The two-photon excited AF was based on the metabolic coenzymes NAD(P)H and flavin adenine dinucleotide/flavoproteins. iPS cells generated from mouse embryonic fibroblasts (MEFs) and cocultured with growth-arrested MEFs as feeder cells have been studied. Significant differences on AF lifetime signatures were identified between iPS and feeder cells as well as between their differentiating counterparts.

  17. 3D confocal imaging in CUBIC-cleared mouse heart

    Energy Technology Data Exchange (ETDEWEB)

    Nehrhoff, I.; Bocancea, D.; Vaquero, J.; Vaquero, J.J.; Lorrio, M.T.; Ripoll, J.; Desco, M.; Gomez-Gaviro, M.V.

    2016-07-01

    Acquiring high resolution 3D images of the heart enables the ability to study heart diseases more in detail. Here, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was adapted for thick mouse heart sections to increase the penetration depth of the confocal microscope lasers into the tissue. The adapted CUBIC clearing of the heart lets the antibody penetrate deeper into the tissue by a factor of five. The here shown protocol enables deep 3D highresolution image acquisition in the heart. This allows a much more accurate assessment of the cellular and structural changes that underlie heart diseases. (Author)

  18. 3D confocal imaging in CUBIC-cleared mouse heart

    International Nuclear Information System (INIS)

    Nehrhoff, I.; Bocancea, D.; Vaquero, J.; Vaquero, J.J.; Lorrio, M.T.; Ripoll, J.; Desco, M.; Gomez-Gaviro, M.V.

    2016-01-01

    Acquiring high resolution 3D images of the heart enables the ability to study heart diseases more in detail. Here, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was adapted for thick mouse heart sections to increase the penetration depth of the confocal microscope lasers into the tissue. The adapted CUBIC clearing of the heart lets the antibody penetrate deeper into the tissue by a factor of five. The here shown protocol enables deep 3D highresolution image acquisition in the heart. This allows a much more accurate assessment of the cellular and structural changes that underlie heart diseases. (Author)

  19. 3D Image Display Courses for Information Media Students.

    Science.gov (United States)

    Yanaka, Kazuhisa; Yamanouchi, Toshiaki

    2016-01-01

    Three-dimensional displays are used extensively in movies and games. These displays are also essential in mixed reality, where virtual and real spaces overlap. Therefore, engineers and creators should be trained to master 3D display technologies. For this reason, the Department of Information Media at the Kanagawa Institute of Technology has launched two 3D image display courses specifically designed for students who aim to become information media engineers and creators.

  20. Surface Explorations : 3D Moving Images as Cartographies of Time

    NARCIS (Netherlands)

    Verhoeff, N.

    2016-01-01

    Moving images of travel and exploration have a long history. In this essay I will examine how the trope of navigation in 3D moving images can work towards an intimate and haptic encounter with other times and other places – elsewhen and elsewhere. The particular navigational construction of space in

  1. Direct trabecular meshwork imaging in porcine eyes through multiphoton gonioscopy

    Science.gov (United States)

    Masihzadeh, Omid; Ammar, David A.; Kahook, Malik Y.; Gibson, Emily A.; Lei, Tim C.

    2013-03-01

    The development of technologies to characterize the ocular aqueous outflow system (AOS) is important for the understanding of the pathophysiology of glaucoma. Multiphoton microscopy (MPM) offers the advantage of high-resolution, label-free imaging with intrinsic image contrast because the emitted signals result from the specific biomolecular content of the tissue. Previous attempts to use MPM to image the murine irido-corneal region directly through the sclera have suffered from degradation in image resolution due to scattering of the focused laser light. As a result, transscleral MPM has limited ability to observe fine structures in the AOS. In this work, the porcine irido-corneal angle was successfully imaged through the transparent cornea using a gonioscopic lens to circumvent the highly scattering scleral tissue. The resulting high-resolution images allowed the detailed structures in the trabecular meshwork (TM) to be observed. Multimodal imaging by two-photon autofluorescence and second harmonic generation allowed visualization of different features in the TM without labels and without disruption of the TM or surrounding tissues. MPM gonioscopy is a promising noninvasive imaging tool for high-resolution studies of the AOS, and research continues to explore the potential for future clinical applications in humans.

  2. Multiphoton gonioscopy to image the trabecular meshwork of porcine eyes

    Science.gov (United States)

    Masihzadeh, Omid; Ammar, David A.; Kahook, Malik Y.; Gibson, Emily A.; Lei, Tim C.

    2013-03-01

    The aqueous outflow system (AOS), including the trabecular meshwork (TM), the collector channels (CC) and the Schlemm's canal (SC), regulates intraocular pressure (IOP) through the drainage of the aqueous humor (AH). Abnormal IOP elevation leads to increased pressure stress to retinal ganglion cells, resulting in cell loss that can ultimately lead to complete loss of eyesight. Therefore, development of imaging tools to detect abnormal structural and functional changes of the AOS is important in early diagnosis and prevention of glaucoma. Multiphoton microscopy (MPM), including twophoton autofluorescence (TPAF) and second harmonic generation (SHG), is a label-free microscopic technique that allows molecular specific imaging of biological tissues like the TM. Since the TM and other AOS structures are located behind the highly scattering scleral tissue, transscleral imaging of the TM does not provide enough optical resolution. In this work, a gonioscopic lens is used to allow direct optical access of the TM through the cornea for MPM imaging. Compared to transscleral imaging, the acquired MPM images show improved resolution as individual collagen fiber bundles of the TM can be observed. MPM gonioscopy may have the potential to be developed as a future clinical imaging tool for glaucoma diagnostics.

  3. Integrated optical 3D digital imaging based on DSP scheme

    Science.gov (United States)

    Wang, Xiaodong; Peng, Xiang; Gao, Bruce Z.

    2008-03-01

    We present a scheme of integrated optical 3-D digital imaging (IO3DI) based on digital signal processor (DSP), which can acquire range images independently without PC support. This scheme is based on a parallel hardware structure with aid of DSP and field programmable gate array (FPGA) to realize 3-D imaging. In this integrated scheme of 3-D imaging, the phase measurement profilometry is adopted. To realize the pipeline processing of the fringe projection, image acquisition and fringe pattern analysis, we present a multi-threads application program that is developed under the environment of DSP/BIOS RTOS (real-time operating system). Since RTOS provides a preemptive kernel and powerful configuration tool, with which we are able to achieve a real-time scheduling and synchronization. To accelerate automatic fringe analysis and phase unwrapping, we make use of the technique of software optimization. The proposed scheme can reach a performance of 39.5 f/s (frames per second), so it may well fit into real-time fringe-pattern analysis and can implement fast 3-D imaging. Experiment results are also presented to show the validity of proposed scheme.

  4. AUTOMATIC 3D MAPPING USING MULTIPLE UNCALIBRATED CLOSE RANGE IMAGES

    Directory of Open Access Journals (Sweden)

    M. Rafiei

    2013-09-01

    Full Text Available Automatic three-dimensions modeling of the real world is an important research topic in the geomatics and computer vision fields for many years. By development of commercial digital cameras and modern image processing techniques, close range photogrammetry is vastly utilized in many fields such as structure measurements, topographic surveying, architectural and archeological surveying, etc. A non-contact photogrammetry provides methods to determine 3D locations of objects from two-dimensional (2D images. Problem of estimating the locations of 3D points from multiple images, often involves simultaneously estimating both 3D geometry (structure and camera pose (motion, it is commonly known as structure from motion (SfM. In this research a step by step approach to generate the 3D point cloud of a scene is considered. After taking images with a camera, we should detect corresponding points in each two views. Here an efficient SIFT method is used for image matching for large baselines. After that, we must retrieve the camera motion and 3D position of the matched feature points up to a projective transformation (projective reconstruction. Lacking additional information on the camera or the scene makes the parallel lines to be unparalleled. The results of SfM computation are much more useful if a metric reconstruction is obtained. Therefor multiple views Euclidean reconstruction applied and discussed. To refine and achieve the precise 3D points we use more general and useful approach, namely bundle adjustment. At the end two real cases have been considered to reconstruct (an excavation and a tower.

  5. PHOTOGRAMMETRIC 3D BUILDING RECONSTRUCTION FROM THERMAL IMAGES

    Directory of Open Access Journals (Sweden)

    E. Maset

    2017-08-01

    Full Text Available This paper addresses the problem of 3D building reconstruction from thermal infrared (TIR images. We show that a commercial Computer Vision software can be used to automatically orient sequences of TIR images taken from an Unmanned Aerial Vehicle (UAV and to generate 3D point clouds, without requiring any GNSS/INS data about position and attitude of the images nor camera calibration parameters. Moreover, we propose a procedure based on Iterative Closest Point (ICP algorithm to create a model that combines high resolution and geometric accuracy of RGB images with the thermal information deriving from TIR images. The process can be carried out entirely by the aforesaid software in a simple and efficient way.

  6. 3D Point Cloud Reconstruction from Single Plenoptic Image

    Directory of Open Access Journals (Sweden)

    F. Murgia

    2016-06-01

    Full Text Available Novel plenoptic cameras sample the light field crossing the main camera lens. The information available in a plenoptic image must be processed, in order to create the depth map of the scene from a single camera shot. In this paper a novel algorithm, for the reconstruction of 3D point cloud of the scene from a single plenoptic image, taken with a consumer plenoptic camera, is proposed. Experimental analysis is conducted on several test images, and results are compared with state of the art methodologies. The results are very promising, as the quality of the 3D point cloud from plenoptic image, is comparable with the quality obtained with current non-plenoptic methodologies, that necessitate more than one image.

  7. AUTOMATED CELL SEGMENTATION WITH 3D FLUORESCENCE MICROSCOPY IMAGES.

    Science.gov (United States)

    Kong, Jun; Wang, Fusheng; Teodoro, George; Liang, Yanhui; Zhu, Yangyang; Tucker-Burden, Carol; Brat, Daniel J

    2015-04-01

    A large number of cell-oriented cancer investigations require an effective and reliable cell segmentation method on three dimensional (3D) fluorescence microscopic images for quantitative analysis of cell biological properties. In this paper, we present a fully automated cell segmentation method that can detect cells from 3D fluorescence microscopic images. Enlightened by fluorescence imaging techniques, we regulated the image gradient field by gradient vector flow (GVF) with interpolated and smoothed data volume, and grouped voxels based on gradient modes identified by tracking GVF field. Adaptive thresholding was then applied to voxels associated with the same gradient mode where voxel intensities were enhanced by a multiscale cell filter. We applied the method to a large volume of 3D fluorescence imaging data of human brain tumor cells with (1) small cell false detection and missing rates for individual cells; and (2) trivial over and under segmentation incidences for clustered cells. Additionally, the concordance of cell morphometry structure between automated and manual segmentation was encouraging. These results suggest a promising 3D cell segmentation method applicable to cancer studies.

  8. Multiphoton imaging with a novel compact diode-pumped Ti:sapphire oscillator

    DEFF Research Database (Denmark)

    König, Karsten; Andersen, Peter E.; Le, Tuan

    2015-01-01

    Multiphoton laser scanning microscopy commonly relies on bulky and expensive femtosecond lasers. We integrated a novel minimal-footprint Ti:sapphire oscillator, pumped by a frequency-doubled distributed Bragg reflector tapered diode laser, into a clinical multiphoton tomograph and evaluated its...... imaging capability using different biological samples, i.e. cell monolayers, corneal tissue, and human skin. With the novel laser, the realization of very compact Ti:sapphire-based systems for high-quality multiphoton imaging at a significantly size and weight compared to current systems will become...

  9. 3D analysis of semiconductor devices: A combination of 3D imaging and 3D elemental analysis

    Science.gov (United States)

    Fu, Bianzhu; Gribelyuk, Michael A.

    2018-04-01

    3D analysis of semiconductor devices using a combination of scanning transmission electron microscopy (STEM) Z-contrast tomography and energy dispersive spectroscopy (EDS) elemental tomography is presented. 3D STEM Z-contrast tomography is useful in revealing the depth information of the sample. However, it suffers from contrast problems between materials with similar atomic numbers. Examples of EDS elemental tomography are presented using an automated EDS tomography system with batch data processing, which greatly reduces the data collection and processing time. 3D EDS elemental tomography reveals more in-depth information about the defect origin in semiconductor failure analysis. The influence of detector shadowing and X-rays absorption on the EDS tomography's result is also discussed.

  10. Automated curved planar reformation of 3D spine images

    International Nuclear Information System (INIS)

    Vrtovec, Tomaz; Likar, Bostjan; Pernus, Franjo

    2005-01-01

    Traditional techniques for visualizing anatomical structures are based on planar cross-sections from volume images, such as images obtained by computed tomography (CT) or magnetic resonance imaging (MRI). However, planar cross-sections taken in the coordinate system of the 3D image often do not provide sufficient or qualitative enough diagnostic information, because planar cross-sections cannot follow curved anatomical structures (e.g. arteries, colon, spine, etc). Therefore, not all of the important details can be shown simultaneously in any planar cross-section. To overcome this problem, reformatted images in the coordinate system of the inspected structure must be created. This operation is usually referred to as curved planar reformation (CPR). In this paper we propose an automated method for CPR of 3D spine images, which is based on the image transformation from the standard image-based to a novel spine-based coordinate system. The axes of the proposed spine-based coordinate system are determined on the curve that represents the vertebral column, and the rotation of the vertebrae around the spine curve, both of which are described by polynomial models. The optimal polynomial parameters are obtained in an image analysis based optimization framework. The proposed method was qualitatively and quantitatively evaluated on five CT spine images. The method performed well on both normal and pathological cases and was consistent with manually obtained ground truth data. The proposed spine-based CPR benefits from reduced structural complexity in favour of improved feature perception of the spine. The reformatted images are diagnostically valuable and enable easier navigation, manipulation and orientation in 3D space. Moreover, reformatted images may prove useful for segmentation and other image analysis tasks

  11. 3D FaceCam: a fast and accurate 3D facial imaging device for biometrics applications

    Science.gov (United States)

    Geng, Jason; Zhuang, Ping; May, Patrick; Yi, Steven; Tunnell, David

    2004-08-01

    Human faces are fundamentally three-dimensional (3D) objects, and each face has its unique 3D geometric profile. The 3D geometric features of a human face can be used, together with its 2D texture, for rapid and accurate face recognition purposes. Due to the lack of low-cost and robust 3D sensors and effective 3D facial recognition (FR) algorithms, almost all existing FR systems use 2D face images. Genex has developed 3D solutions that overcome the inherent problems in 2D while also addressing limitations in other 3D alternatives. One important aspect of our solution is a unique 3D camera (the 3D FaceCam) that combines multiple imaging sensors within a single compact device to provide instantaneous, ear-to-ear coverage of a human face. This 3D camera uses three high-resolution CCD sensors and a color encoded pattern projection system. The RGB color information from each pixel is used to compute the range data and generate an accurate 3D surface map. The imaging system uses no moving parts and combines multiple 3D views to provide detailed and complete 3D coverage of the entire face. Images are captured within a fraction of a second and full-frame 3D data is produced within a few seconds. This described method provides much better data coverage and accuracy in feature areas with sharp features or details (such as the nose and eyes). Using this 3D data, we have been able to demonstrate that a 3D approach can significantly improve the performance of facial recognition. We have conducted tests in which we have varied the lighting conditions and angle of image acquisition in the "field." These tests have shown that the matching results are significantly improved when enrolling a 3D image rather than a single 2D image. With its 3D solutions, Genex is working toward unlocking the promise of powerful 3D FR and transferring FR from a lab technology into a real-world biometric solution.

  12. Autonomous Planetary 3-D Reconstruction From Satellite Images

    DEFF Research Database (Denmark)

    Denver, Troelz

    1999-01-01

    is discussed.Based on such features, 3-D representations may be compiled from two or more 2-D satellite images. The main purposes of such a mapping system are extraction of landing sites, objects of scientific interest and general planetary surveying. All data processing is performed autonomously onboard...

  13. Use of a model for 3D image reconstruction

    International Nuclear Information System (INIS)

    Delageniere, S.; Grangeat, P.

    1991-01-01

    We propose a software for 3D image reconstruction in transmission tomography. This software is based on the use of a model and of the RADON algorithm developed at LETI. The introduction of a markovian model helps us to enhance contrast and straitened the natural transitions existing in the objects studied, whereas standard transform methods smoothe them

  14. Anomaly effects of arrays for 3d geoelectrical resistivity imaging ...

    African Journals Online (AJOL)

    user

    The effectiveness of using a net of orthogonal or parallel sets of two-dimensional (2D) profiles for three- dimensional (3D) geoelectrical resistivity imaging has been evaluated. A series of 2D apparent resistivity data were generated over two synthetic models which represent geological or environmental conditions for a ...

  15. 3D Hyperpolarized C-13 EPI with Calibrationless Parallel Imaging

    DEFF Research Database (Denmark)

    Gordon, Jeremy W.; Hansen, Rie Beck; Shin, Peter J.

    2018-01-01

    With the translation of metabolic MRI with hyperpolarized 13C agents into the clinic, imaging approaches will require large volumetric FOVs to support clinical applications. Parallel imaging techniques will be crucial to increasing volumetric scan coverage while minimizing RF requirements and tem...... strategies to accelerate and undersample hyperpolarized 13C data using 3D blipped EPI acquisitions and multichannel receive coils, and demonstrated its application in a human study of [1-13C]pyruvate metabolism....

  16. Efficient reconfigurable architectures for 3D medical image compression

    OpenAIRE

    Afandi, Ahmad

    2010-01-01

    This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University. Recently, the more widespread use of three-dimensional (3-D) imaging modalities, such as magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and ultrasound (US) have generated a massive amount of volumetric data. These have provided an impetus to the development of other applications, in particular telemedicine and teleradiology. In thes...

  17. 3D Image Fusion to Localise Intercostal Arteries During TEVAR

    Directory of Open Access Journals (Sweden)

    G. Koutouzi

    Full Text Available Purpose: Preservation of intercostal arteries during thoracic aortic procedures reduces the risk of post-operative paraparesis. The origins of the intercostal arteries are visible on pre-operative computed tomography angiography (CTA, but rarely on intra-operative angiography. The purpose of this report is to suggest an image fusion technique for intra-operative localisation of the intercostal arteries during thoracic endovascular repair (TEVAR. Technique: The ostia of the intercostal arteries are identified and manually marked with rings on the pre-operative CTA. The optimal distal landing site in the descending aorta is determined and marked, allowing enough length for an adequate seal and attachment without covering more intercostal arteries than necessary. After 3D/3D fusion of the pre-operative CTA with an intra-operative cone-beam CT (CBCT, the markings are overlaid on the live fluoroscopy screen for guidance. The accuracy of the overlay is confirmed with digital subtraction angiography (DSA and the overlay is adjusted when needed. Stent graft deployment is guided by the markings. The initial experience of this technique in seven patients is presented. Results: 3D image fusion was feasible in all cases. Follow-up CTA after 1 month revealed that all intercostal arteries planned for preservation, were patent. None of the patients developed signs of spinal cord ischaemia. Conclusion: 3D image fusion can be used to localise the intercostal arteries during TEVAR. This may preserve some intercostal arteries and reduce the risk of post-operative spinal cord ischaemia. Keywords: TEVAR, Intercostal artery, Spinal cord ischaemia, 3D image fusion, Image guidance, Cone-beam CT

  18. 3-D repositioning and differential images of volumetric CT measurements

    International Nuclear Information System (INIS)

    Muench, B.; Rueegsegger, P.

    1993-01-01

    In quantitative computed tomography (QCT), time serial measurements are performed to detect a global bone density loss or to identify localized bone density changes. A prerequisite for an unambiguous analysis is the comparison of identical bone volumes. Usually, manual repositioning is too coarse. The authors therefore developed a mathematical procedure that allows matching two three-dimensional image volumes. The algorithm is based on correlation techniques. The procedure has been optimized and applied to computer-tomographic 3-D images of the human knee. It has been tested with both artificially created and in vivo measured image data. Furthermore, typical results of differential images calculated from real bone measurements are presented

  19. An automated 3D reconstruction method of UAV images

    Science.gov (United States)

    Liu, Jun; Wang, He; Liu, Xiaoyang; Li, Feng; Sun, Guangtong; Song, Ping

    2015-10-01

    In this paper a novel fully automated 3D reconstruction approach based on low-altitude unmanned aerial vehicle system (UAVs) images will be presented, which does not require previous camera calibration or any other external prior knowledge. Dense 3D point clouds are generated by integrating orderly feature extraction, image matching, structure from motion (SfM) and multi-view stereo (MVS) algorithms, overcoming many of the cost, time limitations of rigorous photogrammetry techniques. An image topology analysis strategy is introduced to speed up large scene reconstruction by taking advantage of the flight-control data acquired by UAV. Image topology map can significantly reduce the running time of feature matching by limiting the combination of images. A high-resolution digital surface model of the study area is produced base on UAV point clouds by constructing the triangular irregular network. Experimental results show that the proposed approach is robust and feasible for automatic 3D reconstruction of low-altitude UAV images, and has great potential for the acquisition of spatial information at large scales mapping, especially suitable for rapid response and precise modelling in disaster emergency.

  20. Subsurface Profile Mapping using 3-D Compressive Wave Imaging

    Directory of Open Access Journals (Sweden)

    Hazreek Z A M

    2017-01-01

    Full Text Available Geotechnical site investigation related to subsurface profile mapping was commonly performed to provide valuable data for design and construction stage based on conventional drilling techniques. From past experience, drilling techniques particularly using borehole method suffer from limitations related to expensive, time consuming and limited data coverage. Hence, this study performs subsurface profile mapping using 3-D compressive wave imaging in order to minimize those conventional method constraints. Field measurement and data analysis of compressive wave (p-wave, vp was performed using seismic refraction survey (ABEM Terraloc MK 8, 7 kg of sledgehammer and 24 units of vertical geophone and OPTIM (SeisOpt@Picker & SeisOpt@2D software respectively. Then, 3-D compressive wave distribution of subsurface studied was obtained using analysis of SURFER software. Based on 3-D compressive wave image analyzed, it was found that subsurface profile studied consist of three main layers representing top soil (vp = 376 – 600 m/s, weathered material (vp = 900 – 2600 m/s and bedrock (vp > 3000 m/s. Thickness of each layer was varied from 0 – 2 m (first layer, 2 – 20 m (second layer and 20 m and over (third layer. Moreover, groundwater (vp = 1400 – 1600 m/s starts to be detected at 2.0 m depth from ground surface. This study has demonstrated that geotechnical site investigation data related to subsurface profiling was applicable to be obtained using 3-D compressive wave imaging. Furthermore, 3-D compressive wave imaging was performed based on non destructive principle in ground exploration thus consider economic, less time, large data coverage and sustainable to our environment.

  1. Whole-heart coronary MRA with 3D affine motion correction using 3D image-based navigation.

    Science.gov (United States)

    Henningsson, Markus; Prieto, Claudia; Chiribiri, Amedeo; Vaillant, Ghislain; Razavi, Reza; Botnar, René M

    2014-01-01

    Robust motion correction is necessary to minimize respiratory motion artefacts in coronary MR angiography (CMRA). The state-of-the-art method uses a 1D feet-head translational motion correction approach, and data acquisition is limited to a small window in the respiratory cycle, which prolongs the scan by a factor of 2-3. The purpose of this work was to implement 3D affine motion correction for Cartesian whole-heart CMRA using a 3D navigator (3D-NAV) to allow for data acquisition throughout the whole respiratory cycle. 3D affine transformations for different respiratory states (bins) were estimated by using 3D-NAV image acquisitions which were acquired during the startup profiles of a steady-state free precession sequence. The calculated 3D affine transformations were applied to the corresponding high-resolution Cartesian image acquisition which had been similarly binned, to correct for respiratory motion between bins. Quantitative and qualitative comparisons showed no statistical difference between images acquired with the proposed method and the reference method using a diaphragmatic navigator with a narrow gating window. We demonstrate that 3D-NAV and 3D affine correction can be used to acquire Cartesian whole-heart 3D coronary artery images with 100% scan efficiency with similar image quality as with the state-of-the-art gated and corrected method with approximately 50% scan efficiency. Copyright © 2013 Wiley Periodicals, Inc.

  2. Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

    Directory of Open Access Journals (Sweden)

    Seniutinas Gediminas

    2017-06-01

    Full Text Available The evolution of optical microscopy from an imaging technique into a tool for materials modification and fabrication is now being repeated with other characterization techniques, including scanning electron microscopy (SEM, focused ion beam (FIB milling/imaging, and atomic force microscopy (AFM. Fabrication and in situ imaging of materials undergoing a three-dimensional (3D nano-structuring within a 1−100 nm resolution window is required for future manufacturing of devices. This level of precision is critically in enabling the cross-over between different device platforms (e.g. from electronics to micro-/nano-fluidics and/or photonics within future devices that will be interfacing with biological and molecular systems in a 3D fashion. Prospective trends in electron, ion, and nano-tip based fabrication techniques are presented.

  3. 3D Medical Image Interpolation Based on Parametric Cubic Convolution

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In the process of display, manipulation and analysis of biomedical image data, they usually need to be converted to data of isotropic discretization through the process of interpolation, while the cubic convolution interpolation is widely used due to its good tradeoff between computational cost and accuracy. In this paper, we present a whole concept for the 3D medical image interpolation based on cubic convolution, and the six methods, with the different sharp control parameter, which are formulated in details. Furthermore, we also give an objective comparison for these methods using data sets with the different slice spacing. Each slice in these data sets is estimated by each interpolation method and compared with the original slice using three measures: mean-squared difference, number of sites of disagreement, and largest difference. According to the experimental results, we present a recommendation for 3D medical images under the different situations in the end.

  4. Combining Different Modalities for 3D Imaging of Biological Objects

    CERN Document Server

    Tsyganov, E; Kulkarni, P; Mason, R; Parkey, R; Seliuonine, S; Shay, J; Soesbe, T; Zhezher, V; Zinchenko, A I

    2005-01-01

    A resolution enhanced NaI(Tl)-scintillator micro-SPECT device using pinhole collimator geometry has been built and tested with small animals. This device was constructed based on a depth-of-interaction measurement using a thick scintillator crystal and a position sensitive PMT to measure depth-dependent scintillator light profiles. Such a measurement eliminates the parallax error that degrades the high spatial resolution required for small animal imaging. This novel technique for 3D gamma-ray detection was incorporated into the micro-SPECT device and tested with a $^{57}$Co source and $^{98m}$Tc-MDP injected in mice body. To further enhance the investigating power of the tomographic imaging different imaging modalities can be combined. In particular, as proposed and shown in this paper, the optical imaging permits a 3D reconstruction of the animal's skin surface thus improving visualization and making possible depth-dependent corrections, necessary for bioluminescence 3D reconstruction in biological objects. ...

  5. Combining different modalities for 3D imaging of biological objects

    International Nuclear Information System (INIS)

    Tsyganov, Eh.; Antich, P.; Kulkarni, P.; Mason, R.; Parkey, R.; Seliuonine, S.; Shay, J.; Soesbe, T.; Zhezher, V.; Zinchenko, A.

    2005-01-01

    A resolution enhanced NaI(Tl)-scintillator micro-SPECT device using pinhole collimator geometry has been built and tested with small animals. This device was constructed based on a depth-of-interaction measurement using a thick scintillator crystal and a position sensitive PMT to measure depth-dependent scintillator light profiles. Such a measurement eliminates the parallax error that degrades the high spatial resolution required for small animal imaging. This novel technique for 3D gamma-ray detection was incorporated into the micro-SPECT device and tested with a 57 Co source and 98m Tc-MDP injected in mice body. To further enhance the investigating power of the tomographic imaging different imaging modalities can be combined. In particular, as proposed and shown, the optical imaging permits a 3D reconstruction of the animal's skin surface thus improving visualization and making possible depth-dependent corrections, necessary for bioluminescence 3D reconstruction in biological objects. This structural information can provide even more detail if the x-ray tomography is used as presented in the paper

  6. 3D shape recovery from image focus using Gabor features

    Science.gov (United States)

    Mahmood, Fahad; Mahmood, Jawad; Zeb, Ayesha; Iqbal, Javaid

    2018-04-01

    Recovering an accurate and precise depth map from a set of acquired 2-D image dataset of the target object each having different focus information is an ultimate goal of 3-D shape recovery. Focus measure algorithm plays an important role in this architecture as it converts the corresponding color value information into focus information which will be then utilized for recovering depth map. This article introduces Gabor features as focus measure approach for recovering depth map from a set of 2-D images. Frequency and orientation representation of Gabor filter features is similar to human visual system and normally applied for texture representation. Due to its little computational complexity, sharp focus measure curve, robust to random noise sources and accuracy, it is considered as superior alternative to most of recently proposed 3-D shape recovery approaches. This algorithm is deeply investigated on real image sequences and synthetic image dataset. The efficiency of the proposed scheme is also compared with the state of art 3-D shape recovery approaches. Finally, by means of two global statistical measures, root mean square error and correlation, we claim that this approach, in spite of simplicity, generates accurate results.

  7. Transfer, imaging, and analysis plate for facile handling of 384 hanging drop 3D tissue spheroids.

    Science.gov (United States)

    Cavnar, Stephen P; Salomonsson, Emma; Luker, Kathryn E; Luker, Gary D; Takayama, Shuichi

    2014-04-01

    Three-dimensional culture systems bridge the experimental gap between in vivo and in vitro physiology. However, nonstandardized formation and limited downstream adaptability of 3D cultures have hindered mainstream adoption of these systems for biological applications, especially for low- and moderate-throughput assays commonly used in biomedical research. Here we build on our recent development of a 384-well hanging drop plate for spheroid culture to design a complementary spheroid transfer and imaging (TRIM) plate. The low-aspect ratio wells of the TRIM plate facilitated high-fidelity, user-independent, contact-based collection of hanging drop spheroids. Using the TRIM plate, we demonstrated several downstream analyses, including bulk tissue collection for flow cytometry, high-resolution low working-distance immersion imaging, and timely reagent delivery for enzymatic studies. Low working-distance multiphoton imaging revealed a cell type-dependent, macroscopic spheroid structure. Unlike ovarian cancer spheroids, which formed loose, disk-shaped spheroids, human mammary fibroblasts formed tight, spherical, and nutrient-limited spheroids. Beyond the applications we describe here, we expect the hanging drop spheroid plate and complementary TRIM plate to facilitate analyses of spheroids across the spectrum of throughput, particularly for bulk collection of spheroids and high-content imaging.

  8. [3D imaging benefits in clinical pratice of orthodontics].

    Science.gov (United States)

    Frèrejouand, Emmanuel

    2016-12-01

    3D imaging possibilities raised up in the last few years in the orthodontic field. In 2016, it can be used for diagnosis improvement and treatment planning by using digital set up combined to CBCT. It is relevant for orthodontic mechanic updating by creating visible or invisible customised appliances. It forms the basis of numerous scientific researches. The author explains the progress 3D imaging brings to diagnosis and clinics but also highlights the requirements it creates. The daily use of these processes in orthodontic clinical practices needs to be regulated regarding the benefit/risk ratio and the patient satisfaction. The command of the digital work flow created by these technics requires habits modifications from the orthodontist and his staff. © EDP Sciences, SFODF, 2016.

  9. Utilization of multiple frequencies in 3D nonlinear microwave imaging

    DEFF Research Database (Denmark)

    Jensen, Peter Damsgaard; Rubæk, Tonny; Mohr, Johan Jacob

    2012-01-01

    The use of multiple frequencies in a nonlinear microwave algorithm is considered. Using multiple frequencies allows for obtaining the improved resolution available at the higher frequencies while retaining the regularizing effects of the lower frequencies. However, a number of different challenges...... at lower frequencies are used as starting guesses for reconstructions at higher frequencies. The performance is illustrated using simulated 2-D data and data obtained with the 3-D DTU microwave imaging system....

  10. A novel 3D imaging system for strawberry phenotyping

    Directory of Open Access Journals (Sweden)

    Joe Q. He

    2017-11-01

    Full Text Available Abstract Background Accurate and quantitative phenotypic data in plant breeding programmes is vital in breeding to assess the performance of genotypes and to make selections. Traditional strawberry phenotyping relies on the human eye to assess most external fruit quality attributes, which is time-consuming and subjective. 3D imaging is a promising high-throughput technique that allows multiple external fruit quality attributes to be measured simultaneously. Results A low cost multi-view stereo (MVS imaging system was developed, which captured data from 360° around a target strawberry fruit. A 3D point cloud of the sample was derived and analysed with custom-developed software to estimate berry height, length, width, volume, calyx size, colour and achene number. Analysis of these traits in 100 fruits showed good concordance with manual assessment methods. Conclusion This study demonstrates the feasibility of an MVS based 3D imaging system for the rapid and quantitative phenotyping of seven agronomically important external strawberry traits. With further improvement, this method could be applied in strawberry breeding programmes as a cost effective phenotyping technique.

  11. A novel 3D imaging system for strawberry phenotyping.

    Science.gov (United States)

    He, Joe Q; Harrison, Richard J; Li, Bo

    2017-01-01

    Accurate and quantitative phenotypic data in plant breeding programmes is vital in breeding to assess the performance of genotypes and to make selections. Traditional strawberry phenotyping relies on the human eye to assess most external fruit quality attributes, which is time-consuming and subjective. 3D imaging is a promising high-throughput technique that allows multiple external fruit quality attributes to be measured simultaneously. A low cost multi-view stereo (MVS) imaging system was developed, which captured data from 360° around a target strawberry fruit. A 3D point cloud of the sample was derived and analysed with custom-developed software to estimate berry height, length, width, volume, calyx size, colour and achene number. Analysis of these traits in 100 fruits showed good concordance with manual assessment methods. This study demonstrates the feasibility of an MVS based 3D imaging system for the rapid and quantitative phenotyping of seven agronomically important external strawberry traits. With further improvement, this method could be applied in strawberry breeding programmes as a cost effective phenotyping technique.

  12. Automated Identification of Fiducial Points on 3D Torso Images

    Directory of Open Access Journals (Sweden)

    Manas M. Kawale

    2013-01-01

    Full Text Available Breast reconstruction is an important part of the breast cancer treatment process for many women. Recently, 2D and 3D images have been used by plastic surgeons for evaluating surgical outcomes. Distances between different fiducial points are frequently used as quantitative measures for characterizing breast morphology. Fiducial points can be directly marked on subjects for direct anthropometry, or can be manually marked on images. This paper introduces novel algorithms to automate the identification of fiducial points in 3D images. Automating the process will make measurements of breast morphology more reliable, reducing the inter- and intra-observer bias. Algorithms to identify three fiducial points, the nipples, sternal notch, and umbilicus, are described. The algorithms used for localization of these fiducial points are formulated using a combination of surface curvature and 2D color information. Comparison of the 3D coordinates of automatically detected fiducial points and those identified manually, and geodesic distances between the fiducial points are used to validate algorithm performance. The algorithms reliably identified the location of all three of the fiducial points. We dedicate this article to our late colleague and friend, Dr. Elisabeth K. Beahm. Elisabeth was both a talented plastic surgeon and physician-scientist; we deeply miss her insight and her fellowship.

  13. Pavement cracking measurements using 3D laser-scan images

    International Nuclear Information System (INIS)

    Ouyang, W; Xu, B

    2013-01-01

    Pavement condition surveying is vital for pavement maintenance programs that ensure ride quality and traffic safety. This paper first introduces an automated pavement inspection system which uses a three-dimensional (3D) camera and a structured laser light to acquire dense transverse profiles of a pavement lane surface when it carries a moving vehicle. After the calibration, the 3D system can yield a depth resolution of 0.5 mm and a transverse resolution of 1.56 mm pixel −1 at 1.4 m camera height from the ground. The scanning rate of the camera can be set to its maximum at 5000 lines s −1 , allowing the density of scanned profiles to vary with the vehicle's speed. The paper then illustrates the algorithms that utilize 3D information to detect pavement distress, such as transverse, longitudinal and alligator cracking, and presents the field tests on the system's repeatability when scanning a sample pavement in multiple runs at the same vehicle speed, at different vehicle speeds and under different weather conditions. The results show that this dedicated 3D system can capture accurate pavement images that detail surface distress, and obtain consistent crack measurements in repeated tests and under different driving and lighting conditions. (paper)

  14. 3D super-resolution imaging with blinking quantum dots

    Science.gov (United States)

    Wang, Yong; Fruhwirth, Gilbert; Cai, En; Ng, Tony; Selvin, Paul R.

    2013-01-01

    Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots, and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (FWHM) of 8–17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3–7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells. PMID:24093439

  15. Reconstruction, Processing and Display of 3D-Images

    International Nuclear Information System (INIS)

    Lenz, R.

    1986-01-01

    In the last few years a number of methods have been developed which can produce true 3D images, volumes of density values. We review two of these techniques (confocal microscopy and X-ray tomography) which were used in the reconstruction of some of our images. The other images came from transmission electron microscopes, gammacameras and magnetic resonance scanners. A new algorithm is suggested which uses projection onto convex sets to improve the depth resolution in the microscopy case. Since we use a TV-monitor as display device we have to project 3D volumes to 2D images. We use the following type of projections: reprojections, range images, colorcoded depth and shaded surface displays. Shaded surface displays use the surface gradient to compute the gray value in the projection. We describe how this gradient can be computed from the range image and from the original density volume. Normally we compute a whole series of projections where the volume is rotated some degrees between two projections. In a separate display session we can display these images in stereo and motion. We describe how noise reduction filters, gray value transformations, geometric manipulations, gradient filters, texture filters and binary techniques can be used to remove uninteresting points from the volume. Finally, a filter design strategy is developed which is based on the optimal basis function approach by Hummel. We show that for a large class of patterns, in images of arbitrary dimensions, the optimal basis functions are rotation-invariant operators as introduced by Danielsson in the 2D case. We also describe how the orientation of a pattern can be computed from its feature vector. (With 107 refs.) (author)

  16. IMAGE TO POINT CLOUD METHOD OF 3D-MODELING

    Directory of Open Access Journals (Sweden)

    A. G. Chibunichev

    2012-07-01

    Full Text Available This article describes the method of constructing 3D models of objects (buildings, monuments based on digital images and a point cloud obtained by terrestrial laser scanner. The first step is the automated determination of exterior orientation parameters of digital image. We have to find the corresponding points of the image and point cloud to provide this operation. Before the corresponding points searching quasi image of point cloud is generated. After that SIFT algorithm is applied to quasi image and real image. SIFT algorithm allows to find corresponding points. Exterior orientation parameters of image are calculated from corresponding points. The second step is construction of the vector object model. Vectorization is performed by operator of PC in an interactive mode using single image. Spatial coordinates of the model are calculated automatically by cloud points. In addition, there is automatic edge detection with interactive editing available. Edge detection is performed on point cloud and on image with subsequent identification of correct edges. Experimental studies of the method have demonstrated its efficiency in case of building facade modeling.

  17. A filtering approach to image reconstruction in 3D SPECT

    International Nuclear Information System (INIS)

    Bronnikov, Andrei V.

    2000-01-01

    We present a new approach to three-dimensional (3D) image reconstruction using analytical inversion of the exponential divergent beam transform, which can serve as a mathematical model for cone-beam 3D SPECT imaging. We apply a circular cone-beam scan and assume constant attenuation inside a convex area with a known boundary, which is satisfactory in brain imaging. The reconstruction problem is reduced to an image restoration problem characterized by a shift-variant point spread function which is given analytically. The method requires two computation steps: backprojection and filtering. The modulation transfer function (MTF) of the filter is derived by means of an original methodology using the 2D Laplace transform. The filter is implemented in the frequency domain and requires 2D Fourier transform of transverse slices. In order to obtain a shift-invariant cone-beam projection-backprojection operator we resort to an approximation, assuming that the collimator has a relatively large focal length. Nevertheless, numerical experiments demonstrate surprisingly good results for detectors with relatively short focal lengths. The use of a wavelet-based filtering algorithm greatly improves the stability to Poisson noise. (author)

  18. Imaging rat esophagus using combination of reflectance confocal and multiphoton microscopy

    International Nuclear Information System (INIS)

    Zhuo, S M; Chen, J X; Jiang, X S; Lu, K C; Xie, S S

    2008-01-01

    We combine reflectance confocal microscopy (RCM) with multiphoton microscopy (MPM) to image rat esophagus. The two imaging modalities allow detection of layered–resolved complementary information from esophagus. In the keratinizing layer, the keratinocytes boundaries can be characterized by RCM, while the keratinocytes cytoplasm (keratin) can be further imaged by multiphoton autofluorescence signal. In the epithelium, the epithelial cellular boundaries and nucleus can be detected by RCM, and MPM can be used for imaging epithelial cell cytoplasm and monitoring metabolic state of epithelium. In the stroma, multiphoton autofluorescence signal is used to image elastin and second harmonic generation signal is utilized to detect collagen, while RCM is used to determine the optical property of stroma. Overall, these results suggest that the combination of RCM and MPM has potential to provide more important and comprehensive information for early diagnosis of esophageal cancer

  19. Motion robust high resolution 3D free-breathing pulmonary MRI using dynamic 3D image self-navigator.

    Science.gov (United States)

    Jiang, Wenwen; Ong, Frank; Johnson, Kevin M; Nagle, Scott K; Hope, Thomas A; Lustig, Michael; Larson, Peder E Z

    2018-06-01

    To achieve motion robust high resolution 3D free-breathing pulmonary MRI utilizing a novel dynamic 3D image navigator derived directly from imaging data. Five-minute free-breathing scans were acquired with a 3D ultrashort echo time (UTE) sequence with 1.25 mm isotropic resolution. From this data, dynamic 3D self-navigating images were reconstructed under locally low rank (LLR) constraints and used for motion compensation with one of two methods: a soft-gating technique to penalize the respiratory motion induced data inconsistency, and a respiratory motion-resolved technique to provide images of all respiratory motion states. Respiratory motion estimation derived from the proposed dynamic 3D self-navigator of 7.5 mm isotropic reconstruction resolution and a temporal resolution of 300 ms was successful for estimating complex respiratory motion patterns. This estimation improved image quality compared to respiratory belt and DC-based navigators. Respiratory motion compensation with soft-gating and respiratory motion-resolved techniques provided good image quality from highly undersampled data in volunteers and clinical patients. An optimized 3D UTE sequence combined with the proposed reconstruction methods can provide high-resolution motion robust pulmonary MRI. Feasibility was shown in patients who had irregular breathing patterns in which our approach could depict clinically relevant pulmonary pathologies. Magn Reson Med 79:2954-2967, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

  20. Estimation of regional lung expansion via 3D image registration

    Science.gov (United States)

    Pan, Yan; Kumar, Dinesh; Hoffman, Eric A.; Christensen, Gary E.; McLennan, Geoffrey; Song, Joo Hyun; Ross, Alan; Simon, Brett A.; Reinhardt, Joseph M.

    2005-04-01

    A method is described to estimate regional lung expansion and related biomechanical parameters using multiple CT images of the lungs, acquired at different inflation levels. In this study, the lungs of two sheep were imaged utilizing a multi-detector row CT at different lung inflations in the prone and supine positions. Using the lung surfaces and the airway branch points for guidance, a 3D inverse consistent image registration procedure was used to match different lung volumes at each orientation. The registration was validated using a set of implanted metal markers. After registration, the Jacobian of the deformation field was computed to express regional expansion or contraction. The regional lung expansion at different pressures and different orientations are compared.

  1. Multiplexed phase-space imaging for 3D fluorescence microscopy.

    Science.gov (United States)

    Liu, Hsiou-Yuan; Zhong, Jingshan; Waller, Laura

    2017-06-26

    Optical phase-space functions describe spatial and angular information simultaneously; examples of optical phase-space functions include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration removal and 3D reconstruction. High-resolution capture of 4D phase-space datasets is, however, challenging. Previous scanning approaches are slow, light inefficient and do not achieve diffraction-limited resolution. Here, we propose a multiplexed method that solves these problems. We use a spatial light modulator (SLM) in the pupil plane of a microscope in order to sequentially pattern multiplexed coded apertures while capturing images in real space. Then, we reconstruct the 3D fluorescence distribution of our sample by solving an inverse problem via regularized least squares with a proximal accelerated gradient descent solver. We experimentally reconstruct a 101 Megavoxel 3D volume (1010×510×500µm with NA 0.4), demonstrating improved acquisition time, light throughput and resolution compared to scanning aperture methods. Our flexible patterning scheme further allows sparsity in the sample to be exploited for reduced data capture.

  2. Image-Based 3D Face Modeling System

    Directory of Open Access Journals (Sweden)

    Vladimir Vezhnevets

    2005-08-01

    Full Text Available This paper describes an automatic system for 3D face modeling using frontal and profile images taken by an ordinary digital camera. The system consists of four subsystems including frontal feature detection, profile feature detection, shape deformation, and texture generation modules. The frontal and profile feature detection modules automatically extract the facial parts such as the eye, nose, mouth, and ear. The shape deformation module utilizes the detected features to deform the generic head mesh model such that the deformed model coincides with the detected features. A texture is created by combining the facial textures augmented from the input images and the synthesized texture and mapped onto the deformed generic head model. This paper provides a practical system for 3D face modeling, which is highly automated by aggregating, customizing, and optimizing a bunch of individual computer vision algorithms. The experimental results show a highly automated process of modeling, which is sufficiently robust to various imaging conditions. The whole model creation including all the optional manual corrections takes only 2∼3 minutes.

  3. Ultra-realistic 3-D imaging based on colour holography

    International Nuclear Information System (INIS)

    Bjelkhagen, H I

    2013-01-01

    A review of recent progress in colour holography is provided with new applications. Colour holography recording techniques in silver-halide emulsions are discussed. Both analogue, mainly Denisyuk colour holograms, and digitally-printed colour holograms are described and their recent improvements. An alternative to silver-halide materials are the panchromatic photopolymer materials such as the DuPont and Bayer photopolymers which are covered. The light sources used to illuminate the recorded holograms are very important to obtain ultra-realistic 3-D images. In particular the new light sources based on RGB LEDs are described. They show improved image quality over today's commonly used halogen lights. Recent work in colour holography by holographers and companies in different countries around the world are included. To record and display ultra-realistic 3-D images with perfect colour rendering are highly dependent on the correct recording technique using the optimal recording laser wavelengths, the availability of improved panchromatic recording materials and combined with new display light sources.

  4. 3-D brain image registration using optimal morphological processing

    International Nuclear Information System (INIS)

    Loncaric, S.; Dhawan, A.P.

    1994-01-01

    The three-dimensional (3-D) registration of Magnetic Resonance (MR) and Positron Emission Tomographic (PET) images of the brain is important for analysis of the human brain and its diseases. A procedure for optimization of (3-D) morphological structuring elements, based on a genetic algorithm, is presented in the paper. The registration of the MR and PET images is done by means of a registration procedure in two major phases. In the first phase, the Iterative Principal Axis Transform (IPAR) is used for initial registration. In the second phase, the optimal shape description method based on the Morphological Signature Transform (MST) is used for final registration. The morphological processing is used to improve the accuracy of the basic IPAR method. The brain ventricle is used as a landmark for MST registration. A near-optimal structuring element obtained by means of a genetic algorithm is used in MST to describe the shape of the ventricle. The method has been tested on the set of brain images demonstrating the feasibility of approach. (author). 11 refs., 3 figs

  5. 3D painting documentation: evaluation of conservation conditions with 3D imaging and ranging techniques

    Directory of Open Access Journals (Sweden)

    D. Abate

    2014-06-01

    Full Text Available The monitoring of paintings, both on canvas and wooden support, is a crucial issue for the preservation and conservation of this kind of artworks. Many environmental factors (e.g. humidity, temperature, illumination, etc., as well as bad conservation practices (e.g. wrong restorations, inappropriate locations, etc., can compromise the material conditions over time and deteriorate an artwork. The article presents an on-going project realized by a multidisciplinary team composed by the ENEA UTICT 3D GraphLab, the 3D Optical Metrology Unit of the Bruno Kessler Foundation and the Soprintendenza per i Beni Storico Artistici ed Etnoantropologici of Bologna (Italy. The goal of the project is the multi-temporal 3D documentation and monitoring of paintings – at the moment in bad conservation’s situation - and the provision of some metrics to quantify the deformations and damages.

  6. 3D MODEL GENERATION USING OBLIQUE IMAGES ACQUIRED BY UAV

    Directory of Open Access Journals (Sweden)

    A. Lingua

    2017-07-01

    Full Text Available In recent years, many studies revealed the advantages of using airborne oblique images for obtaining improved 3D city models (including façades and building footprints. Here the acquisition and use of oblique images from a low cost and open source Unmanned Aerial Vehicle (UAV for the 3D high-level-of-detail reconstruction of historical architectures is evaluated. The critical issues of such acquisitions (flight planning strategies, ground control points distribution, etc. are described. Several problems should be considered in the flight planning: best approach to cover the whole object with the minimum time of flight; visibility of vertical structures; occlusions due to the context; acquisition of all the parts of the objects (the closest and the farthest with similar resolution; suitable camera inclination, and so on. In this paper a solution is proposed in order to acquire oblique images with one only flight. The data processing was realized using Structure-from-Motion-based approach for point cloud generation using dense image-matching algorithms implemented in an open source software. The achieved results are analysed considering some check points and some reference LiDAR data. The system was tested for surveying a historical architectonical complex: the “Sacro Mo nte di Varallo Sesia” in north-west of Italy. This study demonstrates that the use of oblique images acquired from a low cost UAV system and processed through an open source software is an effective methodology to survey cultural heritage, characterized by limited accessibility, need for detail and rapidity of the acquisition phase, and often reduced budgets.

  7. [EYECUBE as 3D multimedia imaging in macular diagnostics].

    Science.gov (United States)

    Hassenstein, Andrea; Scholz, F; Richard, G

    2011-11-01

    In the new generation of EYECUBE devices, the angiography image and the OCT are included in a 3D illustration as an integration. Other diagnostic procedures such as autofluorescence and ICG can also be correlated to the OCT. The aim was to precisely classify various two-dimensional findings in relation to each other. The new generation of OCT devices enables imaging with a low incidence of motion artefacts with very good fundus image quality - and with that, permits a largely automatic classification. The feature enabling the integration of the EYECUBE was further developed with new software, so that not only the topographic image (red-free, autofluorescence) can be correlated to the Cirrus OCT, but also all other findings gathered within the same time frame can be correlated to each other. These were brightened and projected onto the cube surface in a defined interval. The imaging procedures can be selected in a menu toolbar. Topographic volumetry OCT images can be overlayed. The practical application of the new method was tested on patients with macular disorders. By lightening up the results from various diagnostic procedures, it is possible of late to directly compare pathologies to each other and to the OCT results. In all patients (n = 45 eyes) with good single-image quality, the automated integration into the EYECUBE was possible (to a great extent). The application is not dependent on a certain type of device used in the procedures performed. The increasing level of precision in imaging procedures and the handling of large data volumes has led to the possibility of examining each macular diagnostics procedure from the comparative perspective: imaging (photo) with perfusion (FLA, ICG) and morphology (OCT). The exclusion of motion artefacts and the reliable scan position in the course of the imaging process increases the informative value of OCT. © Georg Thieme Verlag KG Stuttgart · New York.

  8. Extracting 3D layout from a single image using global image structures.

    Science.gov (United States)

    Lou, Zhongyu; Gevers, Theo; Hu, Ninghang

    2015-10-01

    Extracting the pixel-level 3D layout from a single image is important for different applications, such as object localization, image, and video categorization. Traditionally, the 3D layout is derived by solving a pixel-level classification problem. However, the image-level 3D structure can be very beneficial for extracting pixel-level 3D layout since it implies the way how pixels in the image are organized. In this paper, we propose an approach that first predicts the global image structure, and then we use the global structure for fine-grained pixel-level 3D layout extraction. In particular, image features are extracted based on multiple layout templates. We then learn a discriminative model for classifying the global layout at the image-level. Using latent variables, we implicitly model the sublevel semantics of the image, which enrich the expressiveness of our model. After the image-level structure is obtained, it is used as the prior knowledge to infer pixel-wise 3D layout. Experiments show that the results of our model outperform the state-of-the-art methods by 11.7% for 3D structure classification. Moreover, we show that employing the 3D structure prior information yields accurate 3D scene layout segmentation.

  9. Recent progress in 3-D imaging of sea freight containers

    International Nuclear Information System (INIS)

    Fuchs, Theobald; Schön, Tobias; Sukowski, Frank; Dittmann, Jonas; Hanke, Randolf

    2015-01-01

    The inspection of very large objects like sea freight containers with X-ray Computed Tomography (CT) is an emerging technology. A complete 3-D CT scan of a see-freight container takes several hours. Of course, this is too slow to apply it to a large number of containers. However, the benefits of a 3-D CT for sealed freight are obvious: detection of potential threats or illicit cargo without being confronted with legal complications or high time consumption and risks for the security personnel during a manual inspection. Recently distinct progress was made in the field of reconstruction of projections with only a relatively low number of angular positions. Instead of today’s 500 to 1000 rotational steps, as needed for conventional CT reconstruction techniques, this new class of algorithms provides the potential to reduce the number of projection angles approximately by a factor of 10. The main drawback of these advanced iterative methods is the high consumption for numerical processing. But as computational power is getting steadily cheaper, there will be practical applications of these complex algorithms in a foreseeable future. In this paper, we discuss the properties of iterative image reconstruction algorithms and show results of their application to CT of extremely large objects scanning a sea-freight container. A specific test specimen is used to quantitatively evaluate the image quality in terms of spatial and contrast resolution and depending on different number of projections

  10. Acquiring 3D scene information from 2D images

    NARCIS (Netherlands)

    Li, Ping

    2011-01-01

    In recent years, people are becoming increasingly acquainted with 3D technologies such as 3DTV, 3D movies and 3D virtual navigation of city environments in their daily life. Commercial 3D movies are now commonly available for consumers. Virtual navigation of our living environment as used on a

  11. High Resolution 3D Radar Imaging of Comet Interiors

    Science.gov (United States)

    Asphaug, E. I.; Gim, Y.; Belton, M.; Brophy, J.; Weissman, P. R.; Heggy, E.

    2012-12-01

    Knowing the interiors of comets and other primitive bodies is fundamental to our understanding of how planets formed. We have developed a Discovery-class mission formulation, Comet Radar Explorer (CORE), based on the use of previously flown planetary radar sounding techniques, with the goal of obtaining high resolution 3D images of the interior of a small primitive body. We focus on the Jupiter-Family Comets (JFCs) as these are among the most primitive bodies reachable by spacecraft. Scattered in from far beyond Neptune, they are ultimate targets of a cryogenic sample return mission according to the Decadal Survey. Other suitable targets include primitive NEOs, Main Belt Comets, and Jupiter Trojans. The approach is optimal for small icy bodies ~3-20 km diameter with spin periods faster than about 12 hours, since (a) navigation is relatively easy, (b) radar penetration is global for decameter wavelengths, and (c) repeated overlapping ground tracks are obtained. The science mission can be as short as ~1 month for a fast-rotating JFC. Bodies smaller than ~1 km can be globally imaged, but the navigation solutions are less accurate and the relative resolution is coarse. Larger comets are more interesting, but radar signal is unlikely to be reflected from depths greater than ~10 km. So, JFCs are excellent targets for a variety of reasons. We furthermore focus on the use of Solar Electric Propulsion (SEP) to rendezvous shortly after the comet's perihelion. This approach leaves us with ample power for science operations under dormant conditions beyond ~2-3 AU. This leads to a natural mission approach of distant observation, followed by closer inspection, terminated by a dedicated radar mapping orbit. Radar reflections are obtained from a polar orbit about the icy nucleus, which spins underneath. Echoes are obtained from a sounder operating at dual frequencies 5 and 15 MHz, with 1 and 10 MHz bandwidths respectively. The dense network of echoes is used to obtain global 3D

  12. CT-image-based conformal brachytherapy of breast cancer. The significance of semi-3-D and 3-D treatment planning.

    Science.gov (United States)

    Polgár, C; Major, T; Somogyi, A; Takácsi-Nagy, Z; Mangel, L C; Forrai, G; Sulyok, Z; Fodor, J; Németh, G

    2000-03-01

    To compare the conventional 2-D, the simulator-guided semi-3-D and the recently developed CT-guided 3-D brachytherapy treatment planning in the interstitial radiotherapy of breast cancer. In 103 patients with T1-2, N0-1 breast cancer the tumor bed was clipped during breast conserving surgery. Fifty-two of them received boost brachytherapy after 46 to 50 Gy teletherapy and 51 patients were treated with brachytherapy alone via flexible implant tubes. Single, double and triple plane implant was used in 6, 89 and 8 cases, respectively. The dose of boost brachytherapy and sole brachytherapy prescribed to dose reference points was 3 times 4.75 Gy and 7 times 5.2 Gy, respectively. The positions of dose reference points varied according to the level (2-D, semi-3-D and 3-D) of treatment planning performed. The treatment planning was based on the 3-D reconstruction of the surgical clips, implant tubes and skin points. In all cases the implantations were planned with a semi-3-D technique aided by simulator. In 10 cases a recently developed CT-guided 3-D planning system was used. The semi-3-D and 3-D treatment plans were compared to hypothetical 2-D plans using dose-volume histograms and dose non-uniformity ratios. The values of mean central dose, mean skin dose, minimal clip dose, proportion of underdosaged clips and mean target surface dose were evaluated. The accuracy of tumor bed localization and the conformity of planning target volume and treated volume were also analyzed in each technique. With the help of conformal semi-3-D and 3-D brachytherapy planning we could define reference dose points, active source positions and dwell times individually. This technique decreased the mean skin dose with 22.2% and reduced the possibility of geographical miss. We could achieve the best conformity between the planning target volume and the treated volume with the CT-image based 3-D treatment planning, at the cost of worse dose homogeneity. The mean treated volume was reduced by 25

  13. 3D Image Analysis of Geomaterials using Confocal Microscopy

    Science.gov (United States)

    Mulukutla, G.; Proussevitch, A.; Sahagian, D.

    2009-05-01

    Confocal microscopy is one of the most significant advances in optical microscopy of the last century. It is widely used in biological sciences but its application to geomaterials lingers due to a number of technical problems. Potentially the technique can perform non-invasive testing on a laser illuminated sample that fluoresces using a unique optical sectioning capability that rejects out-of-focus light reaching the confocal aperture. Fluorescence in geomaterials is commonly induced using epoxy doped with a fluorochrome that is impregnated into the sample to enable discrimination of various features such as void space or material boundaries. However, for many geomaterials, this method cannot be used because they do not naturally fluoresce and because epoxy cannot be impregnated into inaccessible parts of the sample due to lack of permeability. As a result, the confocal images of most geomaterials that have not been pre-processed with extensive sample preparation techniques are of poor quality and lack the necessary image and edge contrast necessary to apply any commonly used segmentation techniques to conduct any quantitative study of its features such as vesicularity, internal structure, etc. In our present work, we are developing a methodology to conduct a quantitative 3D analysis of images of geomaterials collected using a confocal microscope with minimal amount of prior sample preparation and no addition of fluorescence. Two sample geomaterials, a volcanic melt sample and a crystal chip containing fluid inclusions are used to assess the feasibility of the method. A step-by-step process of image analysis includes application of image filtration to enhance the edges or material interfaces and is based on two segmentation techniques: geodesic active contours and region competition. Both techniques have been applied extensively to the analysis of medical MRI images to segment anatomical structures. Preliminary analysis suggests that there is distortion in the

  14. Improving spatial resolution in quantum imaging beyond the Rayleigh diffraction limit using multiphoton W entangled states

    Energy Technology Data Exchange (ETDEWEB)

    Wen Jianming, E-mail: jianming.wen@gmail.co [National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093 (China); Department of Physics, University of Arkansas, Fayetteville, AR 72701 (United States); Du, Shengwang [Department of Physics, Hong Kong University of Science and Technology, Clear Bay (Hong Kong); Xiao Min [National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093 (China); Department of Physics, University of Arkansas, Fayetteville, AR 72701 (United States); School of Modern Engineering and Applied Science, Nanjing University, Nanjing 210093 (China)

    2010-08-23

    Using multiphoton entangled states, we demonstrate improving spatial imaging resolution beyond the Rayleigh diffraction limit in the quantum imaging process. In particular, we examine resolution enhancement using triphoton W state and a factor of 2 is achievable as with the use of the Greenberger-Horne-Zeilinger state, compared to using a classical-light source.

  15. Web based 3-D medical image visualization on the PC.

    Science.gov (United States)

    Kim, N; Lee, D H; Kim, J H; Kim, Y; Cho, H J

    1998-01-01

    With the recent advance of Web and its associated technologies, information sharing on distribute computing environments has gained a great amount of attention from many researchers in many application areas, such as medicine, engineering, and business. One basic requirement of distributed medical consultation systems is that geographically dispersed, disparate participants are allowed to exchange information readily with each other. Such software also needs to be supported on a broad range of computer platforms to increase the softwares accessibility. In this paper, the development of world-wide-web based medical consultation system for radiology imaging is addressed to provide platform independence and greater accessibility. The system supports sharing of 3-dimensional objects. We use VRML (Virtual Reality Modeling Language), which is the defacto standard in 3-D modeling on the Web. 3-D objects are reconstructed from CT or MRI volume data using a VRML format, which can be viewed and manipulated easily in Web-browsers with a VRML plug-in. A Marching cubes method is used in the transformation of scanned volume data sets to polygonal surfaces of VRML. A decimation algorithm is adopted to reduce the number of meshes in the resulting VRML file. 3-D volume data are often very large in size, hence loading the data on PC level computers requires a significant reduction of the size of the data, while minimizing the loss of the original shape information. This is also important to decrease network delays. A prototype system has been implemented (http://cybernet5.snu.ac.kr/-cyber/mrivrml .html), and several sessions of experiments are carried out.

  16. 3-D Image Analysis of Fluorescent Drug Binding

    Directory of Open Access Journals (Sweden)

    M. Raquel Miquel

    2005-01-01

    Full Text Available Fluorescent ligands provide the means of studying receptors in whole tissues using confocal laser scanning microscopy and have advantages over antibody- or non-fluorescence-based method. Confocal microscopy provides large volumes of images to be measured. Histogram analysis of 3-D image volumes is proposed as a method of graphically displaying large amounts of volumetric image data to be quickly analyzed and compared. The fluorescent ligand BODIPY FL-prazosin (QAPB was used in mouse aorta. Histogram analysis reports the amount of ligand-receptor binding under different conditions and the technique is sensitive enough to detect changes in receptor availability after antagonist incubation or genetic manipulations. QAPB binding was concentration dependent, causing concentration-related rightward shifts in the histogram. In the presence of 10 μM phenoxybenzamine (blocking agent, the QAPB (50 nM histogram overlaps the autofluorescence curve. The histogram obtained for the 1D knockout aorta lay to the left of that of control and 1B knockout aorta, indicating a reduction in 1D receptors. We have shown, for the first time, that it is possible to graphically display binding of a fluorescent drug to a biological tissue. Although our application is specific to adrenergic receptors, the general method could be applied to any volumetric, fluorescence-image-based assay.

  17. Myocardial strains from 3D displacement encoded magnetic resonance imaging

    International Nuclear Information System (INIS)

    Kindberg, Katarina; Haraldsson, Henrik; Sigfridsson, Andreas; Engvall, Jan; Ingels, Neil B Jr; Ebbers, Tino; Karlsson, Matts

    2012-01-01

    The ability to measure and quantify myocardial motion and deformation provides a useful tool to assist in the diagnosis, prognosis and management of heart disease. The recent development of magnetic resonance imaging methods, such as harmonic phase analysis of tagging and displacement encoding with stimulated echoes (DENSE), make detailed non-invasive 3D kinematic analyses of human myocardium possible in the clinic and for research purposes. A robust analysis method is required, however. We propose to estimate strain using a polynomial function which produces local models of the displacement field obtained with DENSE. Given a specific polynomial order, the model is obtained as the least squares fit of the acquired displacement field. These local models are subsequently used to produce estimates of the full strain tensor. The proposed method is evaluated on a numerical phantom as well as in vivo on a healthy human heart. The evaluation showed that the proposed method produced accurate results and showed low sensitivity to noise in the numerical phantom. The method was also demonstrated in vivo by assessment of the full strain tensor and to resolve transmural strain variations. Strain estimation within a 3D myocardial volume based on polynomial functions yields accurate and robust results when validated on an analytical model. The polynomial field is capable of resolving the measured material positions from the in vivo data, and the obtained in vivo strains values agree with previously reported myocardial strains in normal human hearts

  18. Myocardial strains from 3D displacement encoded magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Kindberg Katarina

    2012-04-01

    Full Text Available Abstract Background The ability to measure and quantify myocardial motion and deformation provides a useful tool to assist in the diagnosis, prognosis and management of heart disease. The recent development of magnetic resonance imaging methods, such as harmonic phase analysis of tagging and displacement encoding with stimulated echoes (DENSE, make detailed non-invasive 3D kinematic analyses of human myocardium possible in the clinic and for research purposes. A robust analysis method is required, however. Methods We propose to estimate strain using a polynomial function which produces local models of the displacement field obtained with DENSE. Given a specific polynomial order, the model is obtained as the least squares fit of the acquired displacement field. These local models are subsequently used to produce estimates of the full strain tensor. Results The proposed method is evaluated on a numerical phantom as well as in vivo on a healthy human heart. The evaluation showed that the proposed method produced accurate results and showed low sensitivity to noise in the numerical phantom. The method was also demonstrated in vivo by assessment of the full strain tensor and to resolve transmural strain variations. Conclusions Strain estimation within a 3D myocardial volume based on polynomial functions yields accurate and robust results when validated on an analytical model. The polynomial field is capable of resolving the measured material positions from the in vivo data, and the obtained in vivo strains values agree with previously reported myocardial strains in normal human hearts.

  19. 3D imaging of semiconductor components by discrete laminography

    Energy Technology Data Exchange (ETDEWEB)

    Batenburg, K. J. [Centrum Wiskunde and Informatica, P.O. Box 94079, NL-1090 GB Amsterdam, The Netherlands and iMinds-Vision Lab, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk (Belgium); Palenstijn, W. J.; Sijbers, J. [iMinds-Vision Lab, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk (Belgium)

    2014-06-19

    X-ray laminography is a powerful technique for quality control of semiconductor components. Despite the advantages of nondestructive 3D imaging over 2D techniques based on sectioning, the acquisition time is still a major obstacle for practical use of the technique. In this paper, we consider the application of Discrete Tomography to laminography data, which can potentially reduce the scanning time while still maintaining a high reconstruction quality. By incorporating prior knowledge in the reconstruction algorithm about the materials present in the scanned object, far more accurate reconstructions can be obtained from the same measured data compared to classical reconstruction methods. We present a series of simulation experiments that illustrate the potential of the approach.

  20. 3-D MR imaging of ectopia vasa deferentia

    Energy Technology Data Exchange (ETDEWEB)

    Goenka, Ajit Harishkumar; Parihar, Mohan; Sharma, Raju; Gupta, Arun Kumar [All India Institute of Medical Sciences (AIIMS), Department of Radiology, New Delhi (India); Bhatnagar, Veereshwar [All India Institute of Medical Sciences (AIIMS), Department of Paediatric Surgery, New Delhi (India)

    2009-11-15

    Ectopia vasa deferentia is a complex anomaly characterized by abnormal termination of the urethral end of the vas deferens into the urinary tract due to an incompletely understood developmental error of the distal Wolffian duct. Associated anomalies of the lower gastrointestinal tract and upper urinary tract are also commonly present due to closely related embryological development. Although around 32 cases have been reported in the literature, the MR appearance of this condition has not been previously described. We report a child with high anorectal malformation who was found to have ectopia vasa deferentia, crossed fused renal ectopia and type II caudal regression syndrome on MR examination. In addition to the salient features of this entity on reconstructed MR images, the important role of 3-D MRI in establishing an unequivocal diagnosis and its potential in facilitating individually tailored management is also highlighted. (orig.)

  1. A circular multifocal collimator for 3D SPECT imaging

    International Nuclear Information System (INIS)

    Guillemaud, R.; Grangeat, P.

    1993-01-01

    In order to improve sensitivity of 3D Single Photon Emission Tomography (SPECT) image, a cone-beam collimator can be used. A new circular multifocal collimator is proposed. The multiple focal points are distributed on a transaxial circle which is the trajectory of the focal points during the circular acquisition. This distribution provides a strong focusing at the center of the detector like a cone-beam collimator, with a good sensitivity, and a weak transaxial focusing at the periphery. A solution for an analytical multifocal reconstruction algorithm has been derived. Grangeat algorithm is proposed to use for this purpose in order to reconstruct with a good sensitivity the region of interest. (R.P.) 3 refs

  2. Automatic airline baggage counting using 3D image segmentation

    Science.gov (United States)

    Yin, Deyu; Gao, Qingji; Luo, Qijun

    2017-06-01

    The baggage number needs to be checked automatically during baggage self-check-in. A fast airline baggage counting method is proposed in this paper using image segmentation based on height map which is projected by scanned baggage 3D point cloud. There is height drop in actual edge of baggage so that it can be detected by the edge detection operator. And then closed edge chains are formed from edge lines that is linked by morphological processing. Finally, the number of connected regions segmented by closed chains is taken as the baggage number. Multi-bag experiment that is performed on the condition of different placement modes proves the validity of the method.

  3. 2D-3D image registration in diagnostic and interventional X-Ray imaging

    NARCIS (Netherlands)

    Bom, I.M.J. van der

    2010-01-01

    Clinical procedures that are conventionally guided by 2D x-ray imaging, may benefit from the additional spatial information provided by 3D image data. For instance, guidance of minimally invasive procedures with CT or MRI data provides 3D spatial information and visualization of structures that are

  4. Military efforts in nanosensors, 3D printing, and imaging detection

    Science.gov (United States)

    Edwards, Eugene; Booth, Janice C.; Roberts, J. Keith; Brantley, Christina L.; Crutcher, Sihon H.; Whitley, Michael; Kranz, Michael; Seif, Mohamed; Ruffin, Paul

    2017-04-01

    A team of researchers and support organizations, affiliated with the Army Aviation and Missile Research, Development, and Engineering Center (AMRDEC), has initiated multidiscipline efforts to develop nano-based structures and components for advanced weaponry, aviation, and autonomous air/ground systems applications. The main objective of this research is to exploit unique phenomena for the development of novel technology to enhance warfighter capabilities and produce precision weaponry. The key technology areas that the authors are exploring include nano-based sensors, analysis of 3D printing constituents, and nano-based components for imaging detection. By integrating nano-based devices, structures, and materials into weaponry, the Army can revolutionize existing (and future) weaponry systems by significantly reducing the size, weight, and cost. The major research thrust areas include the development of carbon nanotube sensors to detect rocket motor off-gassing; the application of current methodologies to assess materials used for 3D printing; and the assessment of components to improve imaging seekers. The status of current activities, associated with these key areas and their implementation into AMRDEC's research, is outlined in this paper. Section #2 outlines output data, graphs, and overall evaluations of carbon nanotube sensors placed on a 16 element chip and exposed to various environmental conditions. Section #3 summarizes the experimental results of testing various materials and resulting components that are supplementary to additive manufacturing/fused deposition modeling (FDM). Section #4 recapitulates a preliminary assessment of the optical and electromechanical components of seekers in an effort to propose components and materials that can work more effectively.

  5. GPU-accelerated denoising of 3D magnetic resonance images

    Energy Technology Data Exchange (ETDEWEB)

    Howison, Mark; Wes Bethel, E.

    2014-05-29

    The raw computational power of GPU accelerators enables fast denoising of 3D MR images using bilateral filtering, anisotropic diffusion, and non-local means. In practice, applying these filtering operations requires setting multiple parameters. This study was designed to provide better guidance to practitioners for choosing the most appropriate parameters by answering two questions: what parameters yield the best denoising results in practice? And what tuning is necessary to achieve optimal performance on a modern GPU? To answer the first question, we use two different metrics, mean squared error (MSE) and mean structural similarity (MSSIM), to compare denoising quality against a reference image. Surprisingly, the best improvement in structural similarity with the bilateral filter is achieved with a small stencil size that lies within the range of real-time execution on an NVIDIA Tesla M2050 GPU. Moreover, inappropriate choices for parameters, especially scaling parameters, can yield very poor denoising performance. To answer the second question, we perform an autotuning study to empirically determine optimal memory tiling on the GPU. The variation in these results suggests that such tuning is an essential step in achieving real-time performance. These results have important implications for the real-time application of denoising to MR images in clinical settings that require fast turn-around times.

  6. 3D Power Line Extraction from Multiple Aerial Images

    Directory of Open Access Journals (Sweden)

    Jaehong Oh

    2017-09-01

    Full Text Available Power lines are cables that carry electrical power from a power plant to an electrical substation. They must be connected between the tower structures in such a way that ensures minimum tension and sufficient clearance from the ground. Power lines can stretch and sag with the changing weather, eventually exceeding the planned tolerances. The excessive sags can then cause serious accidents, while hindering the durability of the power lines. We used photogrammetric techniques with a low-cost drone to achieve efficient 3D mapping of power lines that are often difficult to approach. Unlike the conventional image-to-object space approach, we used the object-to-image space approach using cubic grid points. We processed four strips of aerial images to automatically extract the power line points in the object space. Experimental results showed that the approach could successfully extract the positions of the power line points for power line generation and sag measurement with the elevation accuracy of a few centimeters.

  7. 3-D interactive visualisation tools for Hi spectral line imaging

    NARCIS (Netherlands)

    van der Hulst, J. M.; Punzo, D.; Roerdink, J. B. T. M.

    2016-01-01

    Upcoming HI surveys will deliver such large datasets that automated processing using the full 3-D information to find and characterize HI objects is unavoidable. Full 3-D visualization is an essential tool for enabling qualitative and quantitative inspection and analysis of the 3-D data, which is

  8. High resolution 3D imaging of synchrotron generated microbeams

    Energy Technology Data Exchange (ETDEWEB)

    Gagliardi, Frank M., E-mail: frank.gagliardi@wbrc.org.au [Alfred Health Radiation Oncology, The Alfred, Melbourne, Victoria 3004, Australia and School of Medical Sciences, RMIT University, Bundoora, Victoria 3083 (Australia); Cornelius, Iwan [Imaging and Medical Beamline, Australian Synchrotron, Clayton, Victoria 3168, Australia and Centre for Medical Radiation Physics, University of Wollongong, Wollongong, New South Wales 2500 (Australia); Blencowe, Anton [Division of Health Sciences, School of Pharmacy and Medical Sciences, The University of South Australia, Adelaide, South Australia 5000, Australia and Division of Information Technology, Engineering and the Environment, Mawson Institute, University of South Australia, Mawson Lakes, South Australia 5095 (Australia); Franich, Rick D. [School of Applied Sciences and Health Innovations Research Institute, RMIT University, Melbourne, Victoria 3000 (Australia); Geso, Moshi [School of Medical Sciences, RMIT University, Bundoora, Victoria 3083 (Australia)

    2015-12-15

    Purpose: Microbeam radiation therapy (MRT) techniques are under investigation at synchrotrons worldwide. Favourable outcomes from animal and cell culture studies have proven the efficacy of MRT. The aim of MRT researchers currently is to progress to human clinical trials in the near future. The purpose of this study was to demonstrate the high resolution and 3D imaging of synchrotron generated microbeams in PRESAGE® dosimeters using laser fluorescence confocal microscopy. Methods: Water equivalent PRESAGE® dosimeters were fabricated and irradiated with microbeams on the Imaging and Medical Beamline at the Australian Synchrotron. Microbeam arrays comprised of microbeams 25–50 μm wide with 200 or 400 μm peak-to-peak spacing were delivered as single, cross-fire, multidirectional, and interspersed arrays. Imaging of the dosimeters was performed using a NIKON A1 laser fluorescence confocal microscope. Results: The spatial fractionation of the MRT beams was clearly visible in 2D and up to 9 mm in depth. Individual microbeams were easily resolved with the full width at half maximum of microbeams measured on images with resolutions of as low as 0.09 μm/pixel. Profiles obtained demonstrated the change of the peak-to-valley dose ratio for interspersed MRT microbeam arrays and subtle variations in the sample positioning by the sample stage goniometer were measured. Conclusions: Laser fluorescence confocal microscopy of MRT irradiated PRESAGE® dosimeters has been validated in this study as a high resolution imaging tool for the independent spatial and geometrical verification of MRT beam delivery.

  9. High resolution 3D imaging of synchrotron generated microbeams

    International Nuclear Information System (INIS)

    Gagliardi, Frank M.; Cornelius, Iwan; Blencowe, Anton; Franich, Rick D.; Geso, Moshi

    2015-01-01

    Purpose: Microbeam radiation therapy (MRT) techniques are under investigation at synchrotrons worldwide. Favourable outcomes from animal and cell culture studies have proven the efficacy of MRT. The aim of MRT researchers currently is to progress to human clinical trials in the near future. The purpose of this study was to demonstrate the high resolution and 3D imaging of synchrotron generated microbeams in PRESAGE® dosimeters using laser fluorescence confocal microscopy. Methods: Water equivalent PRESAGE® dosimeters were fabricated and irradiated with microbeams on the Imaging and Medical Beamline at the Australian Synchrotron. Microbeam arrays comprised of microbeams 25–50 μm wide with 200 or 400 μm peak-to-peak spacing were delivered as single, cross-fire, multidirectional, and interspersed arrays. Imaging of the dosimeters was performed using a NIKON A1 laser fluorescence confocal microscope. Results: The spatial fractionation of the MRT beams was clearly visible in 2D and up to 9 mm in depth. Individual microbeams were easily resolved with the full width at half maximum of microbeams measured on images with resolutions of as low as 0.09 μm/pixel. Profiles obtained demonstrated the change of the peak-to-valley dose ratio for interspersed MRT microbeam arrays and subtle variations in the sample positioning by the sample stage goniometer were measured. Conclusions: Laser fluorescence confocal microscopy of MRT irradiated PRESAGE® dosimeters has been validated in this study as a high resolution imaging tool for the independent spatial and geometrical verification of MRT beam delivery

  10. CT-image based conformal brachytherapy of breast cancer. The significance of semi-3-D and 3-D treatment planning

    International Nuclear Information System (INIS)

    Polgar, C.; Major, T.; Somogyi, A.; Takacsi-Nagy, Z.; Mangel, L.C.; Fodor, J.; Nemeth, G.; Forrai, G.; Sulyok, Z.

    2000-01-01

    In 103 patients with T1-2, N0-1 breast cancer the tumor bed was clipped during breast conserving surgery. Fifty-two of them received boost brachytherapy after 46 to 50 Gy teletherapy and 51 patients were treated with brachytherapy alone via flexible implant tubes. Single double and triple plane implant was used in 6,89 and 8 cases, respectively. The dose of boost brachytherapy and sole brachytherapy prescribed to dose reference points was 3 times 4.75 Gy and 7 times 5.2 Gy, respectively. The positions of dose reference points varied according to the level (2-D, semi-3-D and 3-D) of treatment planning performed. The treatment planning was based on the 3-D reconstruction of the surgical clips, implant tubes and skin points. In all cases the implantations were planned with a semi-3-D technique aided by simulator. In 10 cases a recently developed CT-guided 3-D planning system was used. The semi-3D and 3-D treatment plans were compared to hypothetical 2-D plans using dose-volume histograms and dose non-uniformity ratios. The values of mean central dose, mean skin dose, minimal clip dose, proportion of underdosaged clips and mean target surface dose were evaluated. The accuracy of tumor bed localization and the conformity of planning target volume and treated volume were also analyzed in each technique. Results: With the help of conformal semi-3D and 3D brachytherapy planning we could define reference dose points, active source positions and dwell times individually. This technique decreased the mean skin dose with 22.2% and reduced the possibility of geographical miss. We could achieve the best conformity between the planning target volume and the treated volume with the CT-image based 3-D treatment planning, at the cost of worse dose homogeneity. The mean treated volume was reduced by 25.1% with semi-3-D planning, however, its was increased by 16.2% with 3-D planning, compared to the 2-D planning. (orig.) [de

  11. Silhouette-based approach of 3D image reconstruction for automated image acquisition using robotic arm

    Science.gov (United States)

    Azhar, N.; Saad, W. H. M.; Manap, N. A.; Saad, N. M.; Syafeeza, A. R.

    2017-06-01

    This study presents the approach of 3D image reconstruction using an autonomous robotic arm for the image acquisition process. A low cost of the automated imaging platform is created using a pair of G15 servo motor connected in series to an Arduino UNO as a main microcontroller. Two sets of sequential images were obtained using different projection angle of the camera. The silhouette-based approach is used in this study for 3D reconstruction from the sequential images captured from several different angles of the object. Other than that, an analysis based on the effect of different number of sequential images on the accuracy of 3D model reconstruction was also carried out with a fixed projection angle of the camera. The effecting elements in the 3D reconstruction are discussed and the overall result of the analysis is concluded according to the prototype of imaging platform.

  12. Self-referenced axial chromatic dispersion measurement in multiphoton microscopy through 2-color THG imaging.

    Science.gov (United States)

    Du, Yu; Zhuang, Ziwei; He, Jiexing; Liu, Hongji; Qiu, Ping; Wang, Ke

    2018-05-16

    With tunable excitation light, multiphoton microscopy (MPM) is widely used for imaging biological structures at subcellular resolution. Axial chromatic dispersion, present in virtually every transmissive optical system including the multiphoton microscope, leads to focal (and the resultant image) plane separation. Here we demonstrate experimentally a technique to measure the axial chromatic dispersion in a multiphoton microscope, using simultaneous 2-color third-harmonic generation (THG) imaging excited by a 2-color soliton source with tunable wavelength separation. Our technique is self-referenced, eliminating potential measurement error when 1-color tunable excitation light is used which necessitates reciprocating motion of the mechanical translation stage. Using this technique, we demonstrate measured axial chromatic dispersion with 2 different objective lenses in a multiphoton microscope. Further measurement in a biological sample also indicates that this axial chromatic dispersion, in combination with 2-color imaging, may open up opportunity for simultaneous imaging of two different axial planes. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  13. 3D Seismic Imaging over a Potential Collapse Structure

    Science.gov (United States)

    Gritto, Roland; O'Connell, Daniel; Elobaid Elnaiem, Ali; Mohamed, Fathelrahman; Sadooni, Fadhil

    2016-04-01

    The Middle-East has seen a recent boom in construction including the planning and development of complete new sub-sections of metropolitan areas. Before planning and construction can commence, however, the development areas need to be investigated to determine their suitability for the planned project. Subsurface parameters such as the type of material (soil/rock), thickness of top soil or rock layers, depth and elastic parameters of basement, for example, comprise important information needed before a decision concerning the suitability of the site for construction can be made. A similar problem arises in environmental impact studies, when subsurface parameters are needed to assess the geological heterogeneity of the subsurface. Environmental impact studies are typically required for each construction project, particularly for the scale of the aforementioned building boom in the Middle East. The current study was conducted in Qatar at the location of a future highway interchange to evaluate a suite of 3D seismic techniques in their effectiveness to interrogate the subsurface for the presence of karst-like collapse structures. The survey comprised an area of approximately 10,000 m2 and consisted of 550 source- and 192 receiver locations. The seismic source was an accelerated weight drop while the geophones consisted of 3-component 10 Hz velocity sensors. At present, we analyzed over 100,000 P-wave phase arrivals and performed high-resolution 3-D tomographic imaging of the shallow subsurface. Furthermore, dispersion analysis of recorded surface waves will be performed to obtain S-wave velocity profiles of the subsurface. Both results, in conjunction with density estimates, will be utilized to determine the elastic moduli of the subsurface rock layers.

  14. Enhanced 3D fluorescence live cell imaging on nanoplasmonic substrate

    International Nuclear Information System (INIS)

    Gartia, Manas Ranjan; Hsiao, Austin; Logan Liu, G; Sivaguru, Mayandi; Chen Yi

    2011-01-01

    We have created a randomly distributed nanocone substrate on silicon coated with silver for surface-plasmon-enhanced fluorescence detection and 3D cell imaging. Optical characterization of the nanocone substrate showed it can support several plasmonic modes (in the 300-800 nm wavelength range) that can be coupled to a fluorophore on the surface of the substrate, which gives rise to the enhanced fluorescence. Spectral analysis suggests that a nanocone substrate can create more excitons and shorter lifetime in the model fluorophore Rhodamine 6G (R6G) due to plasmon resonance energy transfer from the nanocone substrate to the nearby fluorophore. We observed three-dimensional fluorescence enhancement on our substrate shown from the confocal fluorescence imaging of chinese hamster ovary (CHO) cells grown on the substrate. The fluorescence intensity from the fluorophores bound on the cell membrane was amplified more than 100-fold as compared to that on a glass substrate. We believe that strong scattering within the nanostructured area coupled with random scattering inside the cell resulted in the observed three-dimensional enhancement in fluorescence with higher photostability on the substrate surface.

  15. Enhanced 3D fluorescence live cell imaging on nanoplasmonic substrate

    Energy Technology Data Exchange (ETDEWEB)

    Gartia, Manas Ranjan [Department of Nuclear, Plasma and Radiological Engineering, University of Illinois, Urbana, IL 61801 (United States); Hsiao, Austin; Logan Liu, G [Department of Bioengineering, University of Illinois, Urbana, IL 61801 (United States); Sivaguru, Mayandi [Institute for Genomic Biology, University of Illinois, Urbana, IL 61801 (United States); Chen Yi, E-mail: loganliu@illinois.edu [Department of Electrical and Computer Engineering, University of Illinois, Urbana, IL 61801 (United States)

    2011-09-07

    We have created a randomly distributed nanocone substrate on silicon coated with silver for surface-plasmon-enhanced fluorescence detection and 3D cell imaging. Optical characterization of the nanocone substrate showed it can support several plasmonic modes (in the 300-800 nm wavelength range) that can be coupled to a fluorophore on the surface of the substrate, which gives rise to the enhanced fluorescence. Spectral analysis suggests that a nanocone substrate can create more excitons and shorter lifetime in the model fluorophore Rhodamine 6G (R6G) due to plasmon resonance energy transfer from the nanocone substrate to the nearby fluorophore. We observed three-dimensional fluorescence enhancement on our substrate shown from the confocal fluorescence imaging of chinese hamster ovary (CHO) cells grown on the substrate. The fluorescence intensity from the fluorophores bound on the cell membrane was amplified more than 100-fold as compared to that on a glass substrate. We believe that strong scattering within the nanostructured area coupled with random scattering inside the cell resulted in the observed three-dimensional enhancement in fluorescence with higher photostability on the substrate surface.

  16. Potential of ultraviolet widefield imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes

    DEFF Research Database (Denmark)

    Wüstner, Daniel; Brewer, Jonathan R.; Bagatolli, Luis

    2011-01-01

    Dehydroergosterol (DHE) is an intrinsically fluorescent sterol with absorption/emission in the ultraviolet (UV) region and biophysical properties similar to those of cholesterol. We compared the potential of UV-sensitive low-light-level wide-field (UV-WF) imaging with that of multiphoton (MP) exc...

  17. 3-D Imaging by Laser Radar and Applications in Preventing and Combating Crime and Terrorism

    National Research Council Canada - National Science Library

    Letalick, Dietmar; Ahlberg, Joergen; Andersson, Pierre; Chevalier, Tomas; Groenwall, Christina; Larsson, Hakan; Persson, Asa; Klasen, Lena

    2004-01-01

    This paper describes the ongoing research on 3-dimensional (3-D) imaging at FOI. Specifically, we address the new possibilities brought by laser radars, focusing on systems for high resolution 3-D imaging...

  18. Preparing diagnostic 3D images for image registration with planning CT images

    International Nuclear Information System (INIS)

    Tracton, Gregg S.; Miller, Elizabeth P.; Rosenman, Julian; Chang, Sha X.; Sailer, Scott; Boxwala, Azaz; Chaney, Edward L.

    1997-01-01

    Purpose: Pre-radiotherapy (pre-RT) tomographic images acquired for diagnostic purposes often contain important tumor and/or normal tissue information which is poorly defined or absent in planning CT images. Our two years of clinical experience has shown that computer-assisted 3D registration of pre-RT images with planning CT images often plays an indispensable role in accurate treatment volume definition. Often the only available format of the diagnostic images is film from which the original 3D digital data must be reconstructed. In addition, any digital data, whether reconstructed or not, must be put into a form suitable for incorporation into the treatment planning system. The purpose of this investigation was to identify all problems that must be overcome before this data is suitable for clinical use. Materials and Methods: In the past two years we have 3D-reconstructed 300 diagnostic images from film and digital sources. As a problem was discovered we built a software tool to correct it. In time we collected a large set of such tools and found that they must be applied in a specific order to achieve the correct reconstruction. Finally, a toolkit (ediScan) was built that made all these tools available in the proper manner via a pleasant yet efficient mouse-based user interface. Results: Problems we discovered included different magnifications, shifted display centers, non-parallel image planes, image planes not perpendicular to the long axis of the table-top (shearing), irregularly spaced scans, non contiguous scan volumes, multiple slices per film, different orientations for slice axes (e.g. left-right reversal), slices printed at window settings corresponding to tissues of interest for diagnostic purposes, and printing artifacts. We have learned that the specific steps to correct these problems, in order of application, are: Also, we found that fast feedback and large image capacity (at least 2000 x 2000 12-bit pixels) are essential for practical application

  19. Optimized 3D Street Scene Reconstruction from Driving Recorder Images

    Directory of Open Access Journals (Sweden)

    Yongjun Zhang

    2015-07-01

    Full Text Available The paper presents an automatic region detection based method to reconstruct street scenes from driving recorder images. The driving recorder in this paper is a dashboard camera that collects images while the motor vehicle is moving. An enormous number of moving vehicles are included in the collected data because the typical recorders are often mounted in the front of moving vehicles and face the forward direction, which can make matching points on vehicles and guardrails unreliable. Believing that utilizing these image data can reduce street scene reconstruction and updating costs because of their low price, wide use, and extensive shooting coverage, we therefore proposed a new method, which is called the Mask automatic detecting method, to improve the structure results from the motion reconstruction. Note that we define vehicle and guardrail regions as “mask” in this paper since the features on them should be masked out to avoid poor matches. After removing the feature points in our new method, the camera poses and sparse 3D points that are reconstructed with the remaining matches. Our contrast experiments with the typical pipeline of structure from motion (SfM reconstruction methods, such as Photosynth and VisualSFM, demonstrated that the Mask decreased the root-mean-square error (RMSE of the pairwise matching results, which led to more accurate recovering results from the camera-relative poses. Removing features from the Mask also increased the accuracy of point clouds by nearly 30%–40% and corrected the problems of the typical methods on repeatedly reconstructing several buildings when there was only one target building.

  20. Complex adaptation-based LDR image rendering for 3D image reconstruction

    Science.gov (United States)

    Lee, Sung-Hak; Kwon, Hyuk-Ju; Sohng, Kyu-Ik

    2014-07-01

    A low-dynamic tone-compression technique is developed for realistic image rendering that can make three-dimensional (3D) images similar to realistic scenes by overcoming brightness dimming in the 3D display mode. The 3D surround provides varying conditions for image quality, illuminant adaptation, contrast, gamma, color, sharpness, and so on. In general, gain/offset adjustment, gamma compensation, and histogram equalization have performed well in contrast compression; however, as a result of signal saturation and clipping effects, image details are removed and information is lost on bright and dark areas. Thus, an enhanced image mapping technique is proposed based on space-varying image compression. The performance of contrast compression is enhanced with complex adaptation in a 3D viewing surround combining global and local adaptation. Evaluating local image rendering in view of tone and color expression, noise reduction, and edge compensation confirms that the proposed 3D image-mapping model can compensate for the loss of image quality in the 3D mode.

  1. CT-image based conformal brachytherapy of breast cancer. The significance of semi-3-D and 3-D treatment planning

    Energy Technology Data Exchange (ETDEWEB)

    Polgar, C.; Major, T.; Somogyi, A.; Takacsi-Nagy, Z.; Mangel, L.C.; Fodor, J.; Nemeth, G. [Orszagos Onkologiai Intezet, Budapest (Hungary). Dept. of Radiotherapy; Forrai, G. [Haynal Imre Univ. of Health Sciences, Budapest (Hungary). Dept. of Radiology; Sulyok, Z. [Orszagos Onkologiai Intezet, Budapest (Hungary). Dept. of Surgery

    2000-03-01

    In 103 patients with T1-2, N0-1 breast cancer the tumor bed was clipped during breast conserving surgery. Fifty-two of them received boost brachytherapy after 46 to 50 Gy teletherapy and 51 patients were treated with brachytherapy alone via flexible implant tubes. Single double and triple plane implant was used in 6,89 and 8 cases, respectively. The dose of boost brachytherapy and sole brachytherapy prescribed to dose reference points was 3 times 4.75 Gy and 7 times 5.2 Gy, respectively. The positions of dose reference points varied according to the level (2-D, semi-3-D and 3-D) of treatment planning performed. The treatment planning was based on the 3-D reconstruction of the surgical clips, implant tubes and skin points. In all cases the implantations were planned with a semi-3-D technique aided by simulator. In 10 cases a recently developed CT-guided 3-D planning system was used. The semi-3D and 3-D treatment plans were compared to hypothetical 2-D plans using dose-volume histograms and dose non-uniformity ratios. The values of mean central dose, mean skin dose, minimal clip dose, proportion of underdosaged clips and mean target surface dose were evaluated. The accuracy of tumor bed localization and the conformity of planning target volume and treated volume were also analyzed in each technique. Results: With the help of conformal semi-3D and 3D brachytherapy planning we could define reference dose points, active source positions and dwell times individually. This technique decreased the mean skin dose with 22.2% and reduced the possibility of geographical miss. We could achieve the best conformity between the planning target volume and the treated volume with the CT-image based 3-D treatment planning, at the cost of worse dose homogeneity. The mean treated volume was reduced by 25.1% with semi-3-D planning, however, its was increased by 16.2% with 3-D planning, compared to the 2-D planning. (orig.) [German] Bei 103 Patientinnen mit Mammakarzinom der Stadien T1

  2. 3D imaging of nanomaterials by discrete tomography.

    Science.gov (United States)

    Batenburg, K J; Bals, S; Sijbers, J; Kübel, C; Midgley, P A; Hernandez, J C; Kaiser, U; Encina, E R; Coronado, E A; Van Tendeloo, G

    2009-05-01

    The field of discrete tomography focuses on the reconstruction of samples that consist of only a few different materials. Ideally, a three-dimensional (3D) reconstruction of such a sample should contain only one grey level for each of the compositions in the sample. By exploiting this property in the reconstruction algorithm, either the quality of the reconstruction can be improved significantly, or the number of required projection images can be reduced. The discrete reconstruction typically contains fewer artifacts and does not have to be segmented, as it already contains one grey level for each composition. Recently, a new algorithm, called discrete algebraic reconstruction technique (DART), has been proposed that can be used effectively on experimental electron tomography datasets. In this paper, we propose discrete tomography as a general reconstruction method for electron tomography in materials science. We describe the basic principles of DART and show that it can be applied successfully to three different types of samples, consisting of embedded ErSi(2) nanocrystals, a carbon nanotube grown from a catalyst particle and a single gold nanoparticle, respectively.

  3. 3D imaging of nanomaterials by discrete tomography

    International Nuclear Information System (INIS)

    Batenburg, K.J.; Bals, S.; Sijbers, J.; Kuebel, C.; Midgley, P.A.; Hernandez, J.C.; Kaiser, U.; Encina, E.R.; Coronado, E.A.; Van Tendeloo, G.

    2009-01-01

    The field of discrete tomography focuses on the reconstruction of samples that consist of only a few different materials. Ideally, a three-dimensional (3D) reconstruction of such a sample should contain only one grey level for each of the compositions in the sample. By exploiting this property in the reconstruction algorithm, either the quality of the reconstruction can be improved significantly, or the number of required projection images can be reduced. The discrete reconstruction typically contains fewer artifacts and does not have to be segmented, as it already contains one grey level for each composition. Recently, a new algorithm, called discrete algebraic reconstruction technique (DART), has been proposed that can be used effectively on experimental electron tomography datasets. In this paper, we propose discrete tomography as a general reconstruction method for electron tomography in materials science. We describe the basic principles of DART and show that it can be applied successfully to three different types of samples, consisting of embedded ErSi 2 nanocrystals, a carbon nanotube grown from a catalyst particle and a single gold nanoparticle, respectively.

  4. Model-based normalization for iterative 3D PET image

    International Nuclear Information System (INIS)

    Bai, B.; Li, Q.; Asma, E.; Leahy, R.M.; Holdsworth, C.H.; Chatziioannou, A.; Tai, Y.C.

    2002-01-01

    We describe a method for normalization in 3D PET for use with maximum a posteriori (MAP) or other iterative model-based image reconstruction methods. This approach is an extension of previous factored normalization methods in which we include separate factors for detector sensitivity, geometric response, block effects and deadtime. Since our MAP reconstruction approach already models some of the geometric factors in the forward projection, the normalization factors must be modified to account only for effects not already included in the model. We describe a maximum likelihood approach to joint estimation of the count-rate independent normalization factors, which we apply to data from a uniform cylindrical source. We then compute block-wise and block-profile deadtime correction factors using singles and coincidence data, respectively, from a multiframe cylindrical source. We have applied this method for reconstruction of data from the Concorde microPET P4 scanner. Quantitative evaluation of this method using well-counter measurements of activity in a multicompartment phantom compares favourably with normalization based directly on cylindrical source measurements. (author)

  5. Orthodontic treatment plan changed by 3D images

    International Nuclear Information System (INIS)

    Yordanova, G.; Stanimirov, P.

    2014-01-01

    Clinical application of CBCT is most often enforced in dental phenomenon of impacted teeth, hyperodontia, transposition, ankyloses or root resorption and other pathologies in the maxillofacial area. The goal, we put ourselves, is to show how the information from 3D images changes the protocol of the orthodontic treatment. The material, we presented six our clinical cases and the change in the plan of the treatment, which has used after analyzing the information carried on the three planes of CBCT. These cases are casuistic in the orthodontic practice and require individual approach to each of them during their analysis and decision taken. The discussion made by us is in line with reveal of the impacted teeth, where we need to evaluate their vertical depth and mediodistal ratios with the bond structures. At patients with hyperodontia, the assessment is of outmost importance to decide which of the teeth to be extracted and which one to be arranged into the dental arch. The conclusion we make is that diagnostic information is essential for decisions about treatment plan. The exact graphs will lead to better treatment plan and more predictable results. (authors) Key words: CBCT. IMPACTED CANINES. HYPERODONTIA. TRANSPOSITION

  6. Image-Based 3d Reconstruction and Analysis for Orthodontia

    Science.gov (United States)

    Knyaz, V. A.

    2012-08-01

    Among the main tasks of orthodontia are analysis of teeth arches and treatment planning for providing correct position for every tooth. The treatment plan is based on measurement of teeth parameters and designing perfect teeth arch curve which teeth are to create after treatment. The most common technique for teeth moving uses standard brackets which put on teeth and a wire of given shape which is clamped by these brackets for producing necessary forces to every tooth for moving it in given direction. The disadvantages of standard bracket technique are low accuracy of tooth dimensions measurements and problems with applying standard approach for wide variety of complex orthodontic cases. The image-based technique for orthodontic planning, treatment and documenting aimed at overcoming these disadvantages is proposed. The proposed approach provides performing accurate measurements of teeth parameters needed for adequate planning, designing correct teeth position and monitoring treatment process. The developed technique applies photogrammetric means for teeth arch 3D model generation, brackets position determination and teeth shifting analysis.

  7. Perceptual attributes of crosstalk in 3D images

    NARCIS (Netherlands)

    Seuntiëns, P.J.H.; Meesters, L.M.J.; IJsselsteijn, W.A.

    2005-01-01

    Nowadays, crosstalk is probably one of the most annoying distortions in 3D displays. So far, display designers still have a relative lack of knowledge about the relevant subjective attributes of crosstalk and how they are combined in an overall 3D viewing experience model. The aim of the current

  8. Focal switching of photochromic fluorescent proteins enables multiphoton microscopy with superior image contrast.

    Science.gov (United States)

    Kao, Ya-Ting; Zhu, Xinxin; Xu, Fang; Min, Wei

    2012-08-01

    Probing biological structures and functions deep inside live organisms with light is highly desirable. Among the current optical imaging modalities, multiphoton fluorescence microscopy exhibits the best contrast for imaging scattering samples by employing a spatially confined nonlinear excitation. However, as the incident laser power drops exponentially with imaging depth into the sample due to the scattering loss, the out-of-focus background eventually overwhelms the in-focus signal, which defines a fundamental imaging-depth limit. Herein we significantly improve the image contrast for deep scattering samples by harnessing reversibly switchable fluorescent proteins (RSFPs) which can be cycled between bright and dark states upon light illumination. Two distinct techniques, multiphoton deactivation and imaging (MPDI) and multiphoton activation and imaging (MPAI), are demonstrated on tissue phantoms labeled with Dronpa protein. Such a focal switch approach can generate pseudo background-free images. Conceptually different from wave-based approaches that try to reduce light scattering in turbid samples, our work represents a molecule-based strategy that focused on imaging probes.

  9. Deformable M-Reps for 3D Medical Image Segmentation

    Science.gov (United States)

    Pizer, Stephen M.; Fletcher, P. Thomas; Joshi, Sarang; Thall, Andrew; Chen, James Z.; Fridman, Yonatan; Fritsch, Daniel S.; Gash, Graham; Glotzer, John M.; Jiroutek, Michael R.; Lu, Conglin; Muller, Keith E.; Tracton, Gregg; Yushkevich, Paul; Chaney, Edward L.

    2013-01-01

    M-reps (formerly called DSLs) are a multiscale medial means for modeling and rendering 3D solid geometry. They are particularly well suited to model anatomic objects and in particular to capture prior geometric information effectively in deformable models segmentation approaches. The representation is based on figural models, which define objects at coarse scale by a hierarchy of figures – each figure generally a slab representing a solid region and its boundary simultaneously. This paper focuses on the use of single figure models to segment objects of relatively simple structure. A single figure is a sheet of medial atoms, which is interpolated from the model formed by a net, i.e., a mesh or chain, of medial atoms (hence the name m-reps), each atom modeling a solid region via not only a position and a width but also a local figural frame giving figural directions and an object angle between opposing, corresponding positions on the boundary implied by the m-rep. The special capability of an m-rep is to provide spatial and orientational correspondence between an object in two different states of deformation. This ability is central to effective measurement of both geometric typicality and geometry to image match, the two terms of the objective function optimized in segmentation by deformable models. The other ability of m-reps central to effective segmentation is their ability to support segmentation at multiple levels of scale, with successively finer precision. Objects modeled by single figures are segmented first by a similarity transform augmented by object elongation, then by adjustment of each medial atom, and finally by displacing a dense sampling of the m-rep implied boundary. While these models and approaches also exist in 2D, we focus on 3D objects. The segmentation of the kidney from CT and the hippocampus from MRI serve as the major examples in this paper. The accuracy of segmentation as compared to manual, slice-by-slice segmentation is reported. PMID

  10. Wavelength dependent photoelectron circular dichroism of limonene studied by femtosecond multiphoton laser ionization and electron-ion coincidence imaging

    Science.gov (United States)

    Rafiee Fanood, Mohammad M.; Janssen, Maurice H. M.; Powis, Ivan

    2016-09-01

    Enantiomers of the monoterpene limonene have been investigated by (2 + 1) resonance enhanced multiphoton ionization and photoelectron circular dichroism employing tuneable, circularly polarized femtosecond laser pulses. Electron imaging detection provides 3D momentum measurement while electron-ion coincidence detection can be used to mass-tag individual electrons. Additional filtering, by accepting only parent ion tagged electrons, can be then used to provide discrimination against higher energy dissociative ionization mechanisms where more than three photons are absorbed to better delineate the two photon resonant, one photon ionization pathway. The promotion of different vibrational levels and, tentatively, different electronic ion core configurations in the intermediate Rydberg states can be achieved with different laser excitation wavelengths (420 nm, 412 nm, and 392 nm), in turn producing different state distributions in the resulting cations. Strong chiral asymmetries in the lab frame photoelectron angular distributions are quantified, and a comparison made with a single photon (synchrotron radiation) measurement at an equivalent photon energy.

  11. Rainbow Particle Imaging Velocimetry for Dense 3D Fluid Velocity Imaging

    KAUST Repository

    Xiong, Jinhui

    2017-04-11

    Despite significant recent progress, dense, time-resolved imaging of complex, non-stationary 3D flow velocities remains an elusive goal. In this work we tackle this problem by extending an established 2D method, Particle Imaging Velocimetry, to three dimensions by encoding depth into color. The encoding is achieved by illuminating the flow volume with a continuum of light planes (a “rainbow”), such that each depth corresponds to a specific wavelength of light. A diffractive component in the camera optics ensures that all planes are in focus simultaneously. For reconstruction, we derive an image formation model for recovering stationary 3D particle positions. 3D velocity estimation is achieved with a variant of 3D optical flow that accounts for both physical constraints as well as the rainbow image formation model. We evaluate our method with both simulations and an experimental prototype setup.

  12. Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin

    Directory of Open Access Journals (Sweden)

    Stefania Seidenari

    2012-01-01

    Full Text Available Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM, is generated by the fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures and ex vivo samples. Although still limited to the clinical research field, the development of multiphoton laser microscopy is thought to become suitable for a practical application in the next few years: in this paper, we performed an accurate review of the studies published so far, considering the possible fields of application of this imaging method and providing high quality images acquired in the Department of Dermatology of the University of Modena.

  13. Holographic Image Plane Projection Integral 3D Display, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — In response to NASA's need for a 3D virtual reality environment providing scientific data visualization without special user devices, Physical Optics Corporation...

  14. GammaModeler 3-D gamma-ray imaging technology

    International Nuclear Information System (INIS)

    2000-01-01

    The 3-D GammaModelertrademark system was used to survey a portion of the facility and provide 3-D visual and radiation representation of contaminated equipment located within the facility. The 3-D GammaModelertrademark system software was used to deconvolve extended sources into a series of point sources, locate the positions of these sources in space and calculate the 30 cm. dose rates for each of these sources. Localization of the sources in three dimensions provides information on source locations interior to the visual objects and provides a better estimate of the source intensities. The three dimensional representation of the objects can be made transparent in order to visualize sources located within the objects. Positional knowledge of all the sources can be used to calculate a map of the radiation in the canyon. The use of 3-D visual and gamma ray information supports improved planning decision-making, and aids in communications with regulators and stakeholders

  15. Image Reconstruction Based Modeling of 3D Textile Composite (Postprint)

    National Research Council Canada - National Science Library

    Zhou, Eric; Mollenhauer, David; Iarve, Endel

    2007-01-01

    ... joints, near-net shape processing, etc. To fully understand the mechanical behavior of 3-D textile composites, it is essential to perform analyses to predict effective material properties and damage initiation and growth...

  16. 3-D Imaging Systems for Agricultural Applications—A Review

    Directory of Open Access Journals (Sweden)

    Manuel Vázquez-Arellano

    2016-04-01

    Full Text Available Efficiency increase of resources through automation of agriculture requires more information about the production process, as well as process and machinery status. Sensors are necessary for monitoring the status and condition of production by recognizing the surrounding structures such as objects, field structures, natural or artificial markers, and obstacles. Currently, three dimensional (3-D sensors are economically affordable and technologically advanced to a great extent, so a breakthrough is already possible if enough research projects are commercialized. The aim of this review paper is to investigate the state-of-the-art of 3-D vision systems in agriculture, and the role and value that only 3-D data can have to provide information about environmental structures based on the recent progress in optical 3-D sensors. The structure of this research consists of an overview of the different optical 3-D vision techniques, based on the basic principles. Afterwards, their application in agriculture are reviewed. The main focus lays on vehicle navigation, and crop and animal husbandry. The depth dimension brought by 3-D sensors provides key information that greatly facilitates the implementation of automation and robotics in agriculture.

  17. A Method for Interactive 3D Reconstruction of Piecewise Planar Objects from Single Images

    OpenAIRE

    Sturm , Peter; Maybank , Steve

    1999-01-01

    International audience; We present an approach for 3D reconstruction of objects from a single image. Obviously, constraints on the 3D structure are needed to perform this task. Our approach is based on user-provided coplanarity, perpendicularity and parallelism constraints. These are used to calibrate the image and perform 3D reconstruction. The method is described in detail and results are provided.

  18. Multimodal Registration and Fusion for 3D Thermal Imaging

    Directory of Open Access Journals (Sweden)

    Moulay A. Akhloufi

    2015-01-01

    Full Text Available 3D vision is an area of computer vision that has attracted a lot of research interest and has been widely studied. In recent years we witness an increasing interest from the industrial community. This interest is driven by the recent advances in 3D technologies, which enable high precision measurements at an affordable cost. With 3D vision techniques we can conduct advanced manufactured parts inspections and metrology analysis. However, we are not able to detect subsurface defects. This kind of detection is achieved by other techniques, like infrared thermography. In this work, we present a new registration framework for 3D and thermal infrared multimodal fusion. The resulting fused data can be used for advanced 3D inspection in Nondestructive Testing and Evaluation (NDT&E applications. The fusion permits the simultaneous visible surface and subsurface inspections to be conducted in the same process. Experimental tests were conducted with different materials. The obtained results are promising and show how these new techniques can be used efficiently in a combined NDT&E-Metrology analysis of manufactured parts, in areas such as aerospace and automotive.

  19. Contributions in compression of 3D medical images and 2D images; Contributions en compression d'images medicales 3D et d'images naturelles 2D

    Energy Technology Data Exchange (ETDEWEB)

    Gaudeau, Y

    2006-12-15

    The huge amounts of volumetric data generated by current medical imaging techniques in the context of an increasing demand for long term archiving solutions, as well as the rapid development of distant radiology make the use of compression inevitable. Indeed, if the medical community has sided until now with compression without losses, most of applications suffer from compression ratios which are too low with this kind of compression. In this context, compression with acceptable losses could be the most appropriate answer. So, we propose a new loss coding scheme based on 3D (3 dimensional) Wavelet Transform and Dead Zone Lattice Vector Quantization 3D (DZLVQ) for medical images. Our algorithm has been evaluated on several computerized tomography (CT) and magnetic resonance image volumes. The main contribution of this work is the design of a multidimensional dead zone which enables to take into account correlations between neighbouring elementary volumes. At high compression ratios, we show that it can out-perform visually and numerically the best existing methods. These promising results are confirmed on head CT by two medical patricians. The second contribution of this document assesses the effect with-loss image compression on CAD (Computer-Aided Decision) detection performance of solid lung nodules. This work on 120 significant lungs images shows that detection did not suffer until 48:1 compression and still was robust at 96:1. The last contribution consists in the complexity reduction of our compression scheme. The first allocation dedicated to 2D DZLVQ uses an exponential of the rate-distortion (R-D) functions. The second allocation for 2D and 3D medical images is based on block statistical model to estimate the R-D curves. These R-D models are based on the joint distribution of wavelet vectors using a multidimensional mixture of generalized Gaussian (MMGG) densities. (author)

  20. In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke

    DEFF Research Database (Denmark)

    Fumagalli, Stefano; Coles, Jonathan A; Ejlerskov, Patrick

    2011-01-01

    To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion.......To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion....

  1. Quantitative 3-D imaging topogrammetry for telemedicine applications

    Science.gov (United States)

    Altschuler, Bruce R.

    1994-01-01

    The technology to reliably transmit high-resolution visual imagery over short to medium distances in real time has led to the serious considerations of the use of telemedicine, telepresence, and telerobotics in the delivery of health care. These concepts may involve, and evolve toward: consultation from remote expert teaching centers; diagnosis; triage; real-time remote advice to the surgeon; and real-time remote surgical instrument manipulation (telerobotics with virtual reality). Further extrapolation leads to teledesign and telereplication of spare surgical parts through quantitative teleimaging of 3-D surfaces tied to CAD/CAM devices and an artificially intelligent archival data base of 'normal' shapes. The ability to generate 'topogrames' or 3-D surface numerical tables of coordinate values capable of creating computer-generated virtual holographic-like displays, machine part replication, and statistical diagnostic shape assessment is critical to the progression of telemedicine. Any virtual reality simulation will remain in 'video-game' realm until realistic dimensional and spatial relational inputs from real measurements in vivo during surgeries are added to an ever-growing statistical data archive. The challenges of managing and interpreting this 3-D data base, which would include radiographic and surface quantitative data, are considerable. As technology drives toward dynamic and continuous 3-D surface measurements, presenting millions of X, Y, Z data points per second of flexing, stretching, moving human organs, the knowledge base and interpretive capabilities of 'brilliant robots' to work as a surgeon's tireless assistants becomes imaginable. The brilliant robot would 'see' what the surgeon sees--and more, for the robot could quantify its 3-D sensing and would 'see' in a wider spectral range than humans, and could zoom its 'eyes' from the macro world to long-distance microscopy. Unerring robot hands could rapidly perform machine-aided suturing with

  2. 3D ultrasound imaging : Fast and cost-effective morphometry of musculoskeletal tissue

    NARCIS (Netherlands)

    Weide, Guido; Van Der Zwaard, Stephan; Huijing, Peter A.; Jaspers, Richard T.; Harlaar, Jaap

    2017-01-01

    The developmental goal of 3D ultrasound imaging (3DUS) is to engineer a modality to perform 3D morphological ultrasound analysis of human muscles. 3DUS images are constructed from calibrated freehand 2D B-mode ultrasound images, which are positioned into a voxel array. Ultrasound (US) imaging allows

  3. 3D visualization of medical images for personalized learning of human anatomy

    NARCIS (Netherlands)

    Laurence Alpay; Jelle Scheurleer; Harmen Bijwaard

    2015-01-01

    to be held in Lisbon/Portugal on October 15-17, 2015 Medical imaging nowadays often yields high definition 3D images (from CT, PET, MRI, etc.). Usually these images need to be evaluated on 2D monitors. In the transition from 3D to 2D the image becomes more difficult to interpret as a whole. To aid

  4. In vivo stepwise multi-photon activation fluorescence imaging of melanin in human skin

    Science.gov (United States)

    Lai, Zhenhua; Gu, Zetong; Abbas, Saleh; Lowe, Jared; Sierra, Heidy; Rajadhyaksha, Milind; DiMarzio, Charles

    2014-03-01

    The stepwise multi-photon activated fluorescence (SMPAF) of melanin is a low cost and reliable method of detecting melanin because the activation and excitation can be a continuous-wave (CW) mode near infrared (NIR) laser. Our previous work has demonstrated the melanin SMPAF images in sepia melanin, mouse hair, and mouse skin. In this study, we show the feasibility of using SMPAF to detect melanin in vivo. in vivo melanin SMPAF images of normal skin and benign nevus are demonstrated. SMPAF images add specificity for melanin detection than MPFM images and CRM images. Melanin SMPAF is a promising technology to enable early detection of melanoma for dermatologists.

  5. 3D Imaging Technology’s Narrative Appropriation in Cinema

    NARCIS (Netherlands)

    Kiss, Miklós; van den Oever, Annie; Fossati, Giovanna

    2016-01-01

    This chapter traces the cinematic history of stereoscopy by focusing on the contemporary dispute about the values of 3D technology, which are seen as either mere visual attraction or as a technique that perfects the cinematic illusion through increasing perceptual immersion. By taking a neutral

  6. Aerial 3D display by use of a 3D-shaped screen with aerial imaging by retro-reflection (AIRR)

    Science.gov (United States)

    Kurokawa, Nao; Ito, Shusei; Yamamoto, Hirotsugu

    2017-06-01

    The purpose of this paper is to realize an aerial 3D display. We design optical system that employs a projector below a retro-reflector and a 3D-shaped screen. A floating 3D image is formed with aerial imaging by retro-reflection (AIRR). Our proposed system is composed of a 3D-shaped screen, a projector, a quarter-wave retarder, a retro-reflector, and a reflective polarizer. Because AIRR forms aerial images that are plane-symmetric of the light sources regarding the reflective polarizer, the shape of the 3D screen is inverted from a desired aerial 3D image. In order to expand viewing angle, the 3D-shaped screen is surrounded by a retro-reflector. In order to separate the aerial image from reflected lights on the retro- reflector surface, the retro-reflector is tilted by 30 degrees. A projector is located below the retro-reflector at the same height of the 3D-shaped screen. The optical axis of the projector is orthogonal to the 3D-shaped screen. Scattered light on the 3D-shaped screen forms the aerial 3D image. In order to demonstrate the proposed optical design, a corner-cube-shaped screen is used for the 3D-shaped screen. Thus, the aerial 3D image is a cube that is floating above the reflective polarizer. For example, an aerial green cube is formed by projecting a calculated image on the 3D-shaped screen. The green cube image is digitally inverted in depth by our developed software. Thus, we have succeeded in forming aerial 3D image with our designed optical system.

  7. Using multiphoton fluorescence lifetime imaging to characterize liver damage and fluorescein disposition in liver in vivo

    Science.gov (United States)

    Thorling, Camilla A.; Studier, Hauke; Crawford, Darrell; Roberts, Michael S.

    2016-03-01

    Liver disease is the fifth most common cause of death and unlike many other major causes of mortality, liver disease rates are increasing rather than decreasing. There is no ideal measurement of liver disease and although biopsies are the gold standard, this only allows for a spot examination and cannot follow dynamic processes of the liver. Intravital imaging has the potential to extract detailed information over a larger sampling area continuously. The aim of this project was to investigate whether multiphoton and fluorescence lifetime imaging microscopy could detect early liver damage and to assess whether it could detect changes in metabolism of fluorescein in normal and diseased livers. Four experimental groups were used in this study: 1) control; 2) ischemia reperfusion injury; 3) steatosis and 4) steatosis with ischemia reperfusion injury. Results showed that multiphoton microscopy could visualize morphological changes such as decreased fluorescence of endogenous fluorophores and the presence of lipid droplets, characteristic of steatosis. Fluorescence lifetime imaging microscopy showed increase in NADPH in steatosis with and without ischemia reperfusion injury and could detect changes in metabolism of fluorescein to fluorescein monoglurcuronide, which was impaired in steatosis with ischemia reperfusion injury. These results concluded that the combination of multiphoton microscopy and fluorescence lifetime imaging is a promising method of assessing early stage liver damage and that it can be used to study changes in drug metabolism in the liver as an indication of liver disease and has the potential to replace the traditional static liver biopsy currently used.

  8. Rainbow particle imaging velocimetry for dense 3D fluid velocity imaging

    KAUST Repository

    Xiong, Jinhui

    2017-07-21

    Despite significant recent progress, dense, time-resolved imaging of complex, non-stationary 3D flow velocities remains an elusive goal. In this work we tackle this problem by extending an established 2D method, Particle Imaging Velocimetry, to three dimensions by encoding depth into color. The encoding is achieved by illuminating the flow volume with a continuum of light planes (a

  9. Image-based RSA: Roentgen stereophotogrammetric analysis based on 2D-3D image registration.

    Science.gov (United States)

    de Bruin, P W; Kaptein, B L; Stoel, B C; Reiber, J H C; Rozing, P M; Valstar, E R

    2008-01-01

    Image-based Roentgen stereophotogrammetric analysis (IBRSA) integrates 2D-3D image registration and conventional RSA. Instead of radiopaque RSA bone markers, IBRSA uses 3D CT data, from which digitally reconstructed radiographs (DRRs) are generated. Using 2D-3D image registration, the 3D pose of the CT is iteratively adjusted such that the generated DRRs resemble the 2D RSA images as closely as possible, according to an image matching metric. Effectively, by registering all 2D follow-up moments to the same 3D CT, the CT volume functions as common ground. In two experiments, using RSA and using a micromanipulator as gold standard, IBRSA has been validated on cadaveric and sawbone scapula radiographs, and good matching results have been achieved. The accuracy was: |mu |RSA but higher than in vivo standard RSA. Because IBRSA does not require radiopaque markers, it adds functionality to the RSA method by opening new directions and possibilities for research, such as dynamic analyses using fluoroscopy on subjects without markers and computer navigation applications.

  10. 3D fingerprint imaging system based on full-field fringe projection profilometry

    Science.gov (United States)

    Huang, Shujun; Zhang, Zonghua; Zhao, Yan; Dai, Jie; Chen, Chao; Xu, Yongjia; Zhang, E.; Xie, Lili

    2014-01-01

    As an unique, unchangeable and easily acquired biometrics, fingerprint has been widely studied in academics and applied in many fields over the years. The traditional fingerprint recognition methods are based on the obtained 2D feature of fingerprint. However, fingerprint is a 3D biological characteristic. The mapping from 3D to 2D loses 1D information and causes nonlinear distortion of the captured fingerprint. Therefore, it is becoming more and more important to obtain 3D fingerprint information for recognition. In this paper, a novel 3D fingerprint imaging system is presented based on fringe projection technique to obtain 3D features and the corresponding color texture information. A series of color sinusoidal fringe patterns with optimum three-fringe numbers are projected onto a finger surface. From another viewpoint, the fringe patterns are deformed by the finger surface and captured by a CCD camera. 3D shape data of the finger can be obtained from the captured fringe pattern images. This paper studies the prototype of the 3D fingerprint imaging system, including principle of 3D fingerprint acquisition, hardware design of the 3D imaging system, 3D calibration of the system, and software development. Some experiments are carried out by acquiring several 3D fingerprint data. The experimental results demonstrate the feasibility of the proposed 3D fingerprint imaging system.

  11. 2D vs. 3D imaging in laparoscopic surgery-results of a prospective randomized trial.

    Science.gov (United States)

    Buia, Alexander; Stockhausen, Florian; Filmann, Natalie; Hanisch, Ernst

    2017-12-01

    3D imaging is an upcoming technology in laparoscopic surgery, and recent studies have shown that the modern 3D technique is superior in an experimental setting. However, the first randomized controlled clinical trial in this context dates back to 1998 and showed no significant difference between 2D and 3D visualization using the first 3D generation technique, which is now more than 15 years old. Positive results measured in an experimental setting considering 3D imaging on surgical performance led us to initiate a randomized controlled pragmatic clinical trial to validate our findings in daily clinical routine. Standard laparoscopic operations (cholecystectomy, appendectomy) were preoperatively randomized to a 2D or 3D imaging system. We used a surgical comfort scale (Likert scale) and the Raw NASA Workload TLX for the subjective assessment of 2D and 3D imaging; the duration of surgery was also measured. The results of 3D imaging were statistically significant better than 2D imaging concerning the parameters "own felt safety" and "task efficiency"; the difficulty level of the procedures in the 2D and 3D groups did not differ. Overall, the Raw NASA Workload TLX showed no significance between the groups. 3D imaging could be a possible advantage in laparoscopic surgery. The results of our clinical trial show increased personal felt safety and efficiency of the surgeon using a 3D imaging system. Overall of the procedures, the findings assessed using Likert scales in terms of own felt safety and task efficiency were statistically significant for 3D imaging. The individually perceived workload assessed with the Raw NASA TLX shows no difference. Although these findings are subjective impressions of the performing surgeons without a clear benefit for 3D technology in clinical outcome, we think that these results show the capability that 3D laparoscopy can have a positive impact while performing laparoscopic procedures.

  12. Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores.

    Science.gov (United States)

    Chen, Zhixing; Wei, Lu; Zhu, Xinxin; Min, Wei

    2012-08-13

    It is highly desirable to be able to optically probe biological activities deep inside live organisms. By employing a spatially confined excitation via a nonlinear transition, multiphoton fluorescence microscopy has become indispensable for imaging scattering samples. However, as the incident laser power drops exponentially with imaging depth due to scattering loss, the out-of-focus fluorescence eventually overwhelms the in-focal signal. The resulting loss of imaging contrast defines a fundamental imaging-depth limit, which cannot be overcome by increasing excitation intensity. Herein we propose to significantly extend this depth limit by multiphoton activation and imaging (MPAI) of photo-activatable fluorophores. The imaging contrast is drastically improved due to the created disparity of bright-dark quantum states in space. We demonstrate this new principle by both analytical theory and experiments on tissue phantoms labeled with synthetic caged fluorescein dye or genetically encodable photoactivatable GFP.

  13. Pseudo-3D Imaging With The DICOM-8

    Science.gov (United States)

    Shalev, S.; Arenson, J.; Kettner, B.

    1985-09-01

    We have developed the DICOM.-8 digital imaging computer for video image acquisition, processing and display. It is a low-cost mobile systems based on a Z80 microcomputer which controls access to two 512 x 512 x 8-bit image planes through a real-time video arithmetic unit. Image presentation capabilities include orthographic images, isometric plots with hidden-line suppression, real-time mask subtraction, binocular red/green stereo, and volumetric imaging with both geometrical and density windows under operator interactive control. Examples are shown for multiplane series of CT images.

  14. A novel multiphoton microscopy images segmentation method based on superpixel and watershed.

    Science.gov (United States)

    Wu, Weilin; Lin, Jinyong; Wang, Shu; Li, Yan; Liu, Mingyu; Liu, Gaoqiang; Cai, Jianyong; Chen, Guannan; Chen, Rong

    2017-04-01

    Multiphoton microscopy (MPM) imaging technique based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) shows fantastic performance for biological imaging. The automatic segmentation of cellular architectural properties for biomedical diagnosis based on MPM images is still a challenging issue. A novel multiphoton microscopy images segmentation method based on superpixels and watershed (MSW) is presented here to provide good segmentation results for MPM images. The proposed method uses SLIC superpixels instead of pixels to analyze MPM images for the first time. The superpixels segmentation based on a new distance metric combined with spatial, CIE Lab color space and phase congruency features, divides the images into patches which keep the details of the cell boundaries. Then the superpixels are used to reconstruct new images by defining an average value of superpixels as image pixels intensity level. Finally, the marker-controlled watershed is utilized to segment the cell boundaries from the reconstructed images. Experimental results show that cellular boundaries can be extracted from MPM images by MSW with higher accuracy and robustness. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Estimating 3D tilt from local image cues in natural scenes

    OpenAIRE

    Burge, Johannes; McCann, Brian C.; Geisler, Wilson S.

    2016-01-01

    Estimating three-dimensional (3D) surface orientation (slant and tilt) is an important first step toward estimating 3D shape. Here, we examine how three local image cues from the same location (disparity gradient, luminance gradient, and dominant texture orientation) should be combined to estimate 3D tilt in natural scenes. We collected a database of natural stereoscopic images with precisely co-registered range images that provide the ground-truth distance at each pixel location. We then ana...

  16. 3D laser imaging for ODOT interstate network at true 1-mm resolution.

    Science.gov (United States)

    2014-12-01

    With the development of 3D laser imaging technology, the latest iteration of : PaveVision3D Ultra can obtain true 1mm resolution 3D data at full-lane coverage in all : three directions at highway speed up to 60MPH. This project provides rapid survey ...

  17. Accuracy of volume measurement using 3D ultrasound and development of CT-3D US image fusion algorithm for prostate cancer radiotherapy

    International Nuclear Information System (INIS)

    Baek, Jihye; Huh, Jangyoung; Hyun An, So; Oh, Yoonjin; Kim, Myungsoo; Kim, DongYoung; Chung, Kwangzoo; Cho, Sungho; Lee, Rena

    2013-01-01

    Purpose: To evaluate the accuracy of measuring volumes using three-dimensional ultrasound (3D US), and to verify the feasibility of the replacement of CT-MR fusion images with CT-3D US in radiotherapy treatment planning. Methods: Phantoms, consisting of water, contrast agent, and agarose, were manufactured. The volume was measured using 3D US, CT, and MR devices. A CT-3D US and MR-3D US image fusion software was developed using the Insight Toolkit library in order to acquire three-dimensional fusion images. The quality of the image fusion was evaluated using metric value and fusion images. Results: Volume measurement, using 3D US, shows a 2.8 ± 1.5% error, 4.4 ± 3.0% error for CT, and 3.1 ± 2.0% error for MR. The results imply that volume measurement using the 3D US devices has a similar accuracy level to that of CT and MR. Three-dimensional image fusion of CT-3D US and MR-3D US was successfully performed using phantom images. Moreover, MR-3D US image fusion was performed using human bladder images. Conclusions: 3D US could be used in the volume measurement of human bladders and prostates. CT-3D US image fusion could be used in monitoring the target position in each fraction of external beam radiation therapy. Moreover, the feasibility of replacing the CT-MR image fusion to the CT-3D US in radiotherapy treatment planning was verified.

  18. [Accuracy of morphological simulation for orthognatic surgery. Assessment of a 3D image fusion software.

    Science.gov (United States)

    Terzic, A; Schouman, T; Scolozzi, P

    2013-08-06

    The CT/CBCT data allows for 3D reconstruction of skeletal and untextured soft tissue volume. 3D stereophotogrammetry technology has strongly improved the quality of facial soft tissue surface texture. The combination of these two technologies allows for an accurate and complete reconstruction. The 3D virtual head may be used for orthognatic surgical planning, virtual surgery, and morphological simulation obtained with a software dedicated to the fusion of 3D photogrammetric and radiological images. The imaging material include: a multi-slice CT scan or broad field CBCT scan, a 3D photogrammetric camera. The operative image processing protocol includes the following steps: 1) pre- and postoperative CT/CBCT scan and 3D photogrammetric image acquisition; 2) 3D image segmentation and fusion of untextured CT/CBCT skin with the preoperative textured facial soft tissue surface of the 3D photogrammetric scan; 3) image fusion of the pre- and postoperative CT/CBCT data set virtual osteotomies, and 3D photogrammetric soft tissue virtual simulation; 4) fusion of virtual simulated 3D photogrammetric and real postoperative images, and assessment of accuracy using a color-coded scale to measure the differences between the two surfaces. Copyright © 2013. Published by Elsevier Masson SAS.

  19. Flash trajectory imaging of target 3D motion

    Science.gov (United States)

    Wang, Xinwei; Zhou, Yan; Fan, Songtao; He, Jun; Liu, Yuliang

    2011-03-01

    We present a flash trajectory imaging technique which can directly obtain target trajectory and realize non-contact measurement of motion parameters by range-gated imaging and time delay integration. Range-gated imaging gives the range of targets and realizes silhouette detection which can directly extract targets from complex background and decrease the complexity of moving target image processing. Time delay integration increases information of one single frame of image so that one can directly gain the moving trajectory. In this paper, we have studied the algorithm about flash trajectory imaging and performed initial experiments which successfully obtained the trajectory of a falling badminton. Our research demonstrates that flash trajectory imaging is an effective approach to imaging target trajectory and can give motion parameters of moving targets.

  20. Towards 3D ultrasound image based soft tissue tracking: a transrectal ultrasound prostate image alignment system.

    Science.gov (United States)

    Baumann, Michael; Mozer, Pierre; Daanen, Vincent; Troccaz, Jocelyne

    2007-01-01

    The emergence of real-time 3D ultrasound (US) makes it possible to consider image-based tracking of subcutaneous soft tissue targets for computer guided diagnosis and therapy. We propose a 3D transrectal US based tracking system for precise prostate biopsy sample localisation. The aim is to improve sample distribution, to enable targeting of unsampled regions for repeated biopsies, and to make post-interventional quality controls possible. Since the patient is not immobilized, since the prostate is mobile and due to the fact that probe movements are only constrained by the rectum during biopsy acquisition, the tracking system must be able to estimate rigid transformations that are beyond the capture range of common image similarity measures. We propose a fast and robust multi-resolution attribute-vector registration approach that combines global and local optimization methods to solve this problem. Global optimization is performed on a probe movement model that reduces the dimensionality of the search space and thus renders optimization efficient. The method was tested on 237 prostate volumes acquired from 14 different patients for 3D to 3D and 3D to orthogonal 2D slices registration. The 3D-3D version of the algorithm converged correctly in 96.7% of all cases in 6.5s with an accuracy of 1.41mm (r.m.s.) and 3.84mm (max). The 3D to slices method yielded a success rate of 88.9% in 2.3s with an accuracy of 1.37mm (r.m.s.) and 4.3mm (max).

  1. Automatic extraction of soft tissues from 3D MRI head images using model driven analysis

    International Nuclear Information System (INIS)

    Jiang, Hao; Yamamoto, Shinji; Imao, Masanao.

    1995-01-01

    This paper presents an automatic extraction system (called TOPS-3D : Top Down Parallel Pattern Recognition System for 3D Images) of soft tissues from 3D MRI head images by using model driven analysis algorithm. As the construction of system TOPS we developed, two concepts have been considered in the design of system TOPS-3D. One is the system having a hierarchical structure of reasoning using model information in higher level, and the other is a parallel image processing structure used to extract plural candidate regions for a destination entity. The new points of system TOPS-3D are as follows. (1) The TOPS-3D is a three-dimensional image analysis system including 3D model construction and 3D image processing techniques. (2) A technique is proposed to increase connectivity between knowledge processing in higher level and image processing in lower level. The technique is realized by applying opening operation of mathematical morphology, in which a structural model function defined in higher level by knowledge representation is immediately used to the filter function of opening operation as image processing in lower level. The system TOPS-3D applied to 3D MRI head images consists of three levels. First and second levels are reasoning part, and third level is image processing part. In experiments, we applied 5 samples of 3D MRI head images with size 128 x 128 x 128 pixels to the system TOPS-3D to extract the regions of soft tissues such as cerebrum, cerebellum and brain stem. From the experimental results, the system is robust for variation of input data by using model information, and the position and shape of soft tissues are extracted corresponding to anatomical structure. (author)

  2. Athermal electron distribution probed by femtosecond multiphoton photoemission from image potential states

    International Nuclear Information System (INIS)

    Ferrini, Gabriele; Giannetti, Claudio; Pagliara, Stefania; Banfi, Francesco; Galimberti, Gianluca; Parmigiani, Fulvio

    2005-01-01

    Image potential states are populated through indirect, scattering-mediated multiphoton absorption induced by femtosecond laser pulses and revealed by single-photon photoemission. The measured effective mass is significantly different from that obtained with direct, resonant population. These features reveal a strong coupling of the electrons residing in the image potential state, outside the solid, with the underlying hot electron population created by the laser pulse. The coupling is mediated by a many-body scattering interaction between the image potential state electrons and bulk electrons in highly excited states

  3. Infrared imaging of the polymer 3D-printing process

    Science.gov (United States)

    Dinwiddie, Ralph B.; Kunc, Vlastimil; Lindal, John M.; Post, Brian; Smith, Rachel J.; Love, Lonnie; Duty, Chad E.

    2014-05-01

    Both mid-wave and long-wave IR cameras are used to measure various temperature profiles in thermoplastic parts as they are printed. Two significantly different 3D-printers are used in this study. The first is a small scale commercially available Solidoodle 3 printer, which prints parts with layer thicknesses on the order of 125μm. The second printer used is a "Big Area Additive Manufacturing" (BAAM) 3D-printer developed at Oak Ridge National Laboratory. The BAAM prints parts with a layer thicknesses of 4.06 mm. Of particular interest is the temperature of the previously deposited layer as the new hot layer is about to be extruded onto it. The two layers are expected have a stronger bond if the temperature of the substrate layer is above the glass transition temperature. This paper describes the measurement technique and results for a study of temperature decay and substrate layer temperature for ABS thermoplastic with and without the addition of chopped carbon fibers.

  4. Imaging system for creating 3D block-face cryo-images of whole mice

    Science.gov (United States)

    Roy, Debashish; Breen, Michael; Salvado, Olivier; Heinzel, Meredith; McKinley, Eliot; Wilson, David

    2006-03-01

    We developed a cryomicrotome/imaging system that provides high resolution, high sensitivity block-face images of whole mice or excised organs, and applied it to a variety of biological applications. With this cryo-imaging system, we sectioned cryo-preserved tissues at 2-40 μm thickness and acquired high resolution brightfield and fluorescence images with microscopic in-plane resolution (as good as 1.2 μm). Brightfield images of normal and pathological anatomy show exquisite detail, especially in the abdominal cavity. Multi-planar reformatting and 3D renderings allow one to interrogate 3D structures. In this report, we present brightfield images of mouse anatomy, as well as 3D renderings of organs. For BPK mice model of polycystic kidney disease, we compared brightfield cryo-images and kidney volumes to MRI. The color images provided greater contrast and resolution of cysts as compared to in vivo MRI. We note that color cryo-images are closer to what a researcher sees in dissection, making it easier for them to interpret image data. The combination of field of view, depth of field, ultra high resolution and color/fluorescence contrast enables cryo-image volumes to provide details that cannot be found through in vivo imaging or other ex vivo optical imaging approaches. We believe that this novel imaging system will have applications that include identification of mouse phenotypes, characterization of diseases like blood vessel disease, kidney disease, and cancer, assessment of drug and gene therapy delivery and efficacy and validation of other imaging modalities.

  5. Optimization of PET image quality by means of 3D data acquisition and iterative image reconstruction

    International Nuclear Information System (INIS)

    Doll, J.; Zaers, J.; Trojan, H.; Bellemann, M.E.; Adam, L.E.; Haberkorn, U.; Brix, G.

    1998-01-01

    The experiments were performed at the latest-generation whole-body PET system ECAT EXACT HR + . For 2D data acquisition, a collimator of thin tungsten septa was positioned in the field-of-view. Prior to image reconstruction, the measured 3D data were sorted into 2D sinograms by using the Fourier rebinning (FORE) algorithm developed by M. Defrise. The standard filtered backprojection (FBP) method and an optimized ML/EM algorithm with overrelaxation for accelerated convergence were employed for image reconstruction. The spatial resolution of both methods as well as the convergence and noise properties of the ML/EM algorithm were studied in phantom measurements. Furthermore, patient data were acquired in the 2D mode as well as in the 3D mode and reconstructed with both techniques. At the same spatial resolution, the ML/EM-reconstructed images showed fewer and less prominent artefacts than the FBP-reconstructed images. The resulting improved detail conspicuously was achieved for the data acquired in the 2D mode as well as in the 3D mode. The best image quality was obtained by iterative 2D reconstruction of 3D data sets which were previously rebinned into 2D sinograms with help of the FORE algorithm. The phantom measurements revealed that 50 iteration steps with the otpimized ML/EM algorithm were sufficient to keep the relative quantitation error below 5%. (orig./MG) [de

  6. High speed display algorithm for 3D medical images using Multi Layer Range Image

    International Nuclear Information System (INIS)

    Ban, Hideyuki; Suzuki, Ryuuichi

    1993-01-01

    We propose high speed algorithm that display 3D voxel images obtained from medical imaging systems such as MRI. This algorithm convert voxel image data to 6 Multi Layer Range Image (MLRI) data, which is an augmentation of the range image data. To avoid the calculation for invisible voxels, the algorithm selects at most 3 MLRI data from 6 in accordance with the view direction. The proposed algorithm displays 256 x 256 x 256 voxel data within 0.6 seconds using 22 MIPS Workstation without a special hardware such as Graphics Engine. Real-time display will be possible on 100 MIPS class Workstation by our algorithm. (author)

  7. Image fusion in craniofacial virtual reality modeling based on CT and 3dMD photogrammetry.

    Science.gov (United States)

    Xin, Pengfei; Yu, Hongbo; Cheng, Huanchong; Shen, Shunyao; Shen, Steve G F

    2013-09-01

    The aim of this study was to demonstrate the feasibility of building a craniofacial virtual reality model by image fusion of 3-dimensional (3D) CT models and 3 dMD stereophotogrammetric facial surface. A CT scan and stereophotography were performed. The 3D CT models were reconstructed by Materialise Mimics software, and the stereophotogrammetric facial surface was reconstructed by 3 dMD patient software. All 3D CT models were exported as Stereo Lithography file format, and the 3 dMD model was exported as Virtual Reality Modeling Language file format. Image registration and fusion were performed in Mimics software. Genetic algorithm was used for precise image fusion alignment with minimum error. The 3D CT models and the 3 dMD stereophotogrammetric facial surface were finally merged into a single file and displayed using Deep Exploration software. Errors between the CT soft tissue model and 3 dMD facial surface were also analyzed. Virtual model based on CT-3 dMD image fusion clearly showed the photorealistic face and bone structures. Image registration errors in virtual face are mainly located in bilateral cheeks and eyeballs, and the errors are more than 1.5 mm. However, the image fusion of whole point cloud sets of CT and 3 dMD is acceptable with a minimum error that is less than 1 mm. The ease of use and high reliability of CT-3 dMD image fusion allows the 3D virtual head to be an accurate, realistic, and widespread tool, and has a great benefit to virtual face model.

  8. D3D augmented reality imaging system: proof of concept in mammography.

    Science.gov (United States)

    Douglas, David B; Petricoin, Emanuel F; Liotta, Lance; Wilson, Eugene

    2016-01-01

    The purpose of this article is to present images from simulated breast microcalcifications and assess the pattern of the microcalcifications with a technical development called "depth 3-dimensional (D3D) augmented reality". A computer, head display unit, joystick, D3D augmented reality software, and an in-house script of simulated data of breast microcalcifications in a ductal distribution were used. No patient data was used and no statistical analysis was performed. The D3D augmented reality system demonstrated stereoscopic depth perception by presenting a unique image to each eye, focal point convergence, head position tracking, 3D cursor, and joystick fly-through. The D3D augmented reality imaging system offers image viewing with depth perception and focal point convergence. The D3D augmented reality system should be tested to determine its utility in clinical practice.

  9. Phase aided 3D imaging and modeling: dedicated systems and case studies

    Science.gov (United States)

    Yin, Yongkai; He, Dong; Liu, Zeyi; Liu, Xiaoli; Peng, Xiang

    2014-05-01

    Dedicated prototype systems for 3D imaging and modeling (3DIM) are presented. The 3D imaging systems are based on the principle of phase-aided active stereo, which have been developed in our laboratory over the past few years. The reported 3D imaging prototypes range from single 3D sensor to a kind of optical measurement network composed of multiple node 3D-sensors. To enable these 3D imaging systems, we briefly discuss the corresponding calibration techniques for both single sensor and multi-sensor optical measurement network, allowing good performance of the 3DIM prototype systems in terms of measurement accuracy and repeatability. Furthermore, two case studies including the generation of high quality color model of movable cultural heritage and photo booth from body scanning are presented to demonstrate our approach.

  10. High Frame Rate Synthetic Aperture 3D Vector Flow Imaging

    DEFF Research Database (Denmark)

    Villagómez Hoyos, Carlos Armando; Holbek, Simon; Stuart, Matthias Bo

    2016-01-01

    , current volumetric ultrasonic flow methods are limited to one velocity component or restricted to a reduced field of view (FOV), e.g. fixed imaging planes, in exchange for higher temporal resolutions. To solve these problems, a previously proposed accurate 2-D high frame rate vector flow imaging (VFI...

  11. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

    CSIR Research Space (South Africa)

    Henriques, R

    2010-05-01

    Full Text Available QuickPALM in conjunction with the acquisition of control features provides a complete solution for the acquisition, reconstruction and visualization of 3D PALM or STORM images, achieving resolutions of ~40 nm in real time. This software package...

  12. The Mathematical Foundations of 3D Compton Scatter Emission Imaging

    Directory of Open Access Journals (Sweden)

    T. T. Truong

    2007-01-01

    Full Text Available The mathematical principles of tomographic imaging using detected (unscattered X- or gamma-rays are based on the two-dimensional Radon transform and many of its variants. In this paper, we show that two new generalizations, called conical Radon transforms, are related to three-dimensional imaging processes based on detected Compton scattered radiation. The first class of conical Radon transform has been introduced recently to support imaging principles of collimated detector systems. The second class is new and is closely related to the Compton camera imaging principles and invertible under special conditions. As they are poised to play a major role in future designs of biomedical imaging systems, we present an account of their most important properties which may be relevant for active researchers in the field.

  13. Advanced 3-D Ultrasound Imaging: 3-D Synthetic Aperture Imaging using Fully Addressed and Row-Column Addressed 2-D Transducer Arrays

    DEFF Research Database (Denmark)

    Bouzari, Hamed

    the important diagnostic information in a noninvasive manner. Diagnostic and therapeutic decisions often require accurate estimates of e.g., organ, cyst, or tumor volumes. 3-D ultrasound imaging can provide these measurements without relying on the geometrical assumptions and operator-dependent skills involved...... is one of the factors for the widespread use of ultrasound imaging. The high price tag on the high quality 3-D scanners is limiting their market share. Row-column addressing of 2-D transducer arrays is a low cost alternative to fully addressed 2-D arrays, for 3-D ultrasound imaging. Using row....... Based on a set of acoustical measurements the center frequency, bandwidth, surface pressure, sensitivity, and acoustical cross-talks were evaluated and discussed. The imaging quality assessments were carried out based on Field II simulations as well as phantom measurements. Moreover, an analysis...

  14. Analytic 3D image reconstruction using all detected events

    International Nuclear Information System (INIS)

    Kinahan, P.E.; Rogers, J.G.

    1988-11-01

    We present the results of testing a previously presented algorithm for three-dimensional image reconstruction that uses all gamma-ray coincidence events detected by a PET volume-imaging scanner. By using two iterations of an analytic filter-backprojection method, the algorithm is not constrained by the requirement of a spatially invariant detector point spread function, which limits normal analytic techniques. Removing this constraint allows the incorporation of all detected events, regardless of orientation, which improves the statistical quality of the final reconstructed image

  15. In vivo multiphoton and fluorescence lifetime imaging microscopy of the healthy and cholestatic liver

    Science.gov (United States)

    Kuznetsova, Daria S.; Dudenkova, Varvara V.; Rodimova, Svetlana A.; Bobrov, Nikolai V.; Zagainov, Vladimir E.; Zagaynova, Elena V.

    2018-02-01

    A cholestatic liver disease presents one of the most common liver diseases and can potentially progress to cirrhosis or even cholangiocarcinoma. Conventional techniques are insufficient to precisely describe the complex internal structure, heterogeneous cell populations and the dynamics of biological processes of the liver. Currently, the methods of multiphoton and fluorescence lifetime imaging microscopy are actively introducing to biomedical research. Those methods are extremely informative and non-destructive that allows studying of a large number of processes occurring inside cells and tissues, analyzing molecular cellular composition, as well as evaluating the state of connective tissue fibers due to their ability to generate a second optical harmonic. Multiphoton and FLIM microscopy do not need additional staining of samples or the incorporation of any markers to study metabolism, lipid composition, microstructure analysis, evaluation of fibrous structures. These parameters have pronounced changes in hepatocytes of liver with common pathological diseases. Thereby in this study we investigated metabolic changes in the healthy and cholestatic liver based on the fluorescence of the metabolic co-factors NAD(P)H and FAD by multiphoton microscopy combined with FLIM. To estimate the contribution of energy metabolism and lipogenesis in the observed changes of the metabolic profile, a separate analysis of NADH and NADPH was presented. The data can be used to develop new criteria for the identification of hepatic pathology at the level of hepatocyte changes directed to personalized medicine in the future.

  16. Multiresolution 3-D reconstruction from side-scan sonar images.

    Science.gov (United States)

    Coiras, Enrique; Petillot, Yvan; Lane, David M

    2007-02-01

    In this paper, a new method for the estimation of seabed elevation maps from side-scan sonar images is presented. The side-scan image formation process is represented by a Lambertian diffuse model, which is then inverted by a multiresolution optimization procedure inspired by expectation-maximization to account for the characteristics of the imaged seafloor region. On convergence of the model, approximations for seabed reflectivity, side-scan beam pattern, and seabed altitude are obtained. The performance of the system is evaluated against a real structure of known dimensions. Reconstruction results for images acquired by different sonar sensors are presented. Applications to augmented reality for the simulation of targets in sonar imagery are also discussed.

  17. Cytology 3D structure formation based on optical microscopy images

    Science.gov (United States)

    Pronichev, A. N.; Polyakov, E. V.; Shabalova, I. P.; Djangirova, T. V.; Zaitsev, S. M.

    2017-01-01

    The article the article is devoted to optimization of the parameters of imaging of biological preparations in optical microscopy using a multispectral camera in visible range of electromagnetic radiation. A model for the image forming of virtual preparations was proposed. The optimum number of layers was determined for the object scan in depth and holistic perception of its switching according to the results of the experiment.

  18. Cytology 3D structure formation based on optical microscopy images

    International Nuclear Information System (INIS)

    Pronichev, A N; Polyakov, E V; Zaitsev, S M; Shabalova, I P; Djangirova, T V

    2017-01-01

    The article the article is devoted to optimization of the parameters of imaging of biological preparations in optical microscopy using a multispectral camera in visible range of electromagnetic radiation. A model for the image forming of virtual preparations was proposed. The optimum number of layers was determined for the object scan in depth and holistic perception of its switching according to the results of the experiment. (paper)

  19. Pragmatic fully 3D image reconstruction for the MiCES mouse imaging PET scanner

    International Nuclear Information System (INIS)

    Lee, Kisung; Kinahan, Paul E; Fessler, Jeffrey A; Miyaoka, Robert S; Janes, Marie; Lewellen, Tom K

    2004-01-01

    We present a pragmatic approach to image reconstruction for data from the micro crystal elements system (MiCES) fully 3D mouse imaging positron emission tomography (PET) scanner under construction at the University of Washington. Our approach is modelled on fully 3D image reconstruction used in clinical PET scanners, which is based on Fourier rebinning (FORE) followed by 2D iterative image reconstruction using ordered-subsets expectation-maximization (OSEM). The use of iterative methods allows modelling of physical effects (e.g., statistical noise, detector blurring, attenuation, etc), while FORE accelerates the reconstruction process by reducing the fully 3D data to a stacked set of independent 2D sinograms. Previous investigations have indicated that non-stationary detector point-spread response effects, which are typically ignored for clinical imaging, significantly impact image quality for the MiCES scanner geometry. To model the effect of non-stationary detector blurring (DB) in the FORE+OSEM(DB) algorithm, we have added a factorized system matrix to the ASPIRE reconstruction library. Initial results indicate that the proposed approach produces an improvement in resolution without an undue increase in noise and without a significant increase in the computational burden. The impact on task performance, however, remains to be evaluated

  20. MR imaging in epilepsy with use of 3D MP-RAGE

    International Nuclear Information System (INIS)

    Tanaka, Akio; Ohno, Sigeru; Sei, Tetsuro; Kanazawa, Susumu; Yasui, Koutaro; Kuroda, Masahiro; Hiraki, Yoshio; Oka, Eiji

    1996-01-01

    The patients were 40 males and 33 females; their ages ranged from 1 month to 39 years (mean: 15.7 years). The patients underwent MR imaging, including spin-echo T 1 -weighted, turbo spin-echo proton density/T 2 -weighted, and 3D magnetization-prepared rapid gradient-echo (3D MP-RAGE) images. These examinations disclosed 39 focal abnormalities. On visual evaluation, the boundary of abnormal gray matter in the neuronal migration disorder (NMD) cases was most clealy shown on 3D MP-RAGE images as compared to the other images. This is considered to be due to the higher spatial resolution and the better contrast of the 3D MP-RAGE images than those of the other techniques. The relative contrast difference between abnormal gray matter and the adjacent white matter was also assessed. The results revealed that the contrast differences on the 3D MP-RAGE images were larger than those on the other images; this was statistically significant. Although the sensitivity of 3D MP-RAGE for NMD was not specifically evaluated in this study, the possibility of this disorder, in cases suspected on other images, could be ruled out. Thus, it appears that the specificity with respect to NMD was at least increased with us of 3D MP-RAGE. 3D MP-RAGE also enabled us to build three-dimensional surface models that were helpful in understanding the three-dimensional anatomy. Furthermore. 3D MP-RAGE was considered to be the best technique for evaluating hippocampus atrophy in patients with MTS. On the other hand, the sensitivity in the signal change of the hippocampus was higher on T 2 -weighted images. In addition, demonstration of cortical tubers of tuberous sclerosis in neurocutaneous syndrome was superior on T 2 -weighted images than on 3D MP-RAGE images. (K.H.)

  1. Naked-eye 3D imaging employing a modified MIMO micro-ring conjugate mirrors

    Science.gov (United States)

    Youplao, P.; Pornsuwancharoen, N.; Amiri, I. S.; Thieu, V. N.; Yupapin, P.

    2018-03-01

    In this work, the use of a micro-conjugate mirror that can produce the 3D image incident probe and display is proposed. By using the proposed system together with the concept of naked-eye 3D imaging, a pixel and a large volume pixel of a 3D image can be created and displayed as naked-eye perception, which is valuable for the large volume naked-eye 3D imaging applications. In operation, a naked-eye 3D image that has a large pixel volume will be constructed by using the MIMO micro-ring conjugate mirror system. Thereafter, these 3D images, formed by the first micro-ring conjugate mirror system, can be transmitted through an optical link to a short distance away and reconstructed via the recovery conjugate mirror at the other end of the transmission. The image transmission is performed by the Fourier integral in MATLAB and compares to the Opti-wave program results. The Fourier convolution is also included for the large volume image transmission. The simulation is used for the manipulation, where the array of a micro-conjugate mirror system is designed and simulated for the MIMO system. The naked-eye 3D imaging is confirmed by the concept of the conjugate mirror in both the input and output images, in terms of the four-wave mixing (FWM), which is discussed and interpreted.

  2. Analysis of information for cerebrovascular disorders obtained by 3D MR imaging

    International Nuclear Information System (INIS)

    Yoshikawa, Kohki; Yoshioka, Naoki; Watanabe, Fumio; Shiono, Takahiro; Sugishita, Morihiro; Umino, Kazunori.

    1995-01-01

    Recently, it becomes easy to analyze information obtained by 3D MR imaging due to remarkable progress of fast MR imaging technique and analysis tool. Six patients suffered from aphasia (4 cerebral infarctions and 2 bleedings) were performed 3D MR imaging (3D FLASH-TR/TE/flip angle; 20-50 msec/6-10 msec/20-30 degrees) and their volume information were analyzed by multiple projection reconstruction (MPR), surface rendering 3D reconstruction, and volume rendering 3D reconstruction using Volume Design PRO (Medical Design Co., Ltd.). Four of them were diagnosed as Broca's aphasia clinically and their lesions could be detected around the cortices of the left inferior frontal gyrus. Another 2 patients were diagnosed as Wernicke's aphasia and the lesions could be detected around the cortices of the left supramarginal gyrus. This technique for 3D volume analyses would provide quite exact locational information about cerebral cortical lesions. (author)

  3. Analysis of information for cerebrovascular disorders obtained by 3D MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikawa, Kohki [Tokyo Univ. (Japan). Inst. of Medical Science; Yoshioka, Naoki; Watanabe, Fumio; Shiono, Takahiro; Sugishita, Morihiro; Umino, Kazunori

    1995-12-01

    Recently, it becomes easy to analyze information obtained by 3D MR imaging due to remarkable progress of fast MR imaging technique and analysis tool. Six patients suffered from aphasia (4 cerebral infarctions and 2 bleedings) were performed 3D MR imaging (3D FLASH-TR/TE/flip angle; 20-50 msec/6-10 msec/20-30 degrees) and their volume information were analyzed by multiple projection reconstruction (MPR), surface rendering 3D reconstruction, and volume rendering 3D reconstruction using Volume Design PRO (Medical Design Co., Ltd.). Four of them were diagnosed as Broca`s aphasia clinically and their lesions could be detected around the cortices of the left inferior frontal gyrus. Another 2 patients were diagnosed as Wernicke`s aphasia and the lesions could be detected around the cortices of the left supramarginal gyrus. This technique for 3D volume analyses would provide quite exact locational information about cerebral cortical lesions. (author).

  4. Radar Imaging of Spheres in 3D using MUSIC

    Energy Technology Data Exchange (ETDEWEB)

    Chambers, D H; Berryman, J G

    2003-01-21

    We have shown that multiple spheres can be imaged by linear and planar EM arrays using only one component of polarization. The imaging approach involves calculating the SVD of the scattering response matrix, selecting a subset of singular values that represents noise, and evaluating the MUSIC functional. The noise threshold applied to the spectrum of singular values for optimal performance is typically around 1%. The resulting signal subspace includes more than one singular value per sphere. The presence of reflections from the ground improves height localization, even for a linear array parallel to the ground. However, the interference between direct and reflected energy modulates the field, creating periodic nulls that can obscure targets in typical images. These nulls are largely eliminated by normalizing the MUSIC functional with the broadside beam pattern of the array. The resulting images show excellent localization for 1 and 2 spheres. The performance for the 3 sphere configurations are complicated by shadowing effects and the greater range of the 3rd sphere in case 2. Two of the three spheres are easily located by MUSIC but the third is difficult to distinguish from other local maxima of the complex imaging functional. Improvement is seen when the linear array is replace with a planar array, which increases the effective aperture height. Further analysis of the singular values and their relationship to modes of scattering from the spheres, as well as better ways to exploit polarization, should improve performance. Work along these lines is currently being pursued by the authors.

  5. Towards functional 3D T-ray imaging

    International Nuclear Information System (INIS)

    Ferguson, Bradley; Wang, Shaohong; Gray, Doug; Abbott, Derek; Zhang, X-C

    2002-01-01

    We review the recent development of T-ray computed tomography, a terahertz imaging technique that allows the reconstruction of the three-dimensional refractive index profile of weakly scattering objects. Terahertz pulse imaging is used to obtain images of the target at multiple projection angles and the filtered backprojection algorithm enables the reconstruction of the object's frequency-dependent refractive index. The application of this technique to a biological bone sample and a plastic test structure is demonstrated. The structure of each target is accurately resolved and the frequency-dependent refractive index is determined. The frequency-dependent information may potentially be used to extract functional information from the target, to uniquely identify different materials or to diagnose medical conditions

  6. A monthly quality assurance procedure for 3D surface imaging.

    Science.gov (United States)

    Wooten, H Omar; Klein, Eric E; Gokhroo, Garima; Santanam, Lakshmi

    2010-12-21

    A procedure for periodic quality assurance of a video surface imaging system is introduced. AlignRT is a video camera-based patient localization system that captures and compares images of a patient's topography to a DICOM-formatted external contour, then calculates shifts required to accurately reposition the patient. This technical note describes the tools and methods implemented in our department to verify correct and accurate operation of the AlignRT hardware and software components. The procedure described is performed monthly and complements a daily calibration of the system.

  7. Effects of intra-operative fluoroscopic 3D-imaging on peri-operative imaging strategy in calcaneal fracture surgery.

    Science.gov (United States)

    Beerekamp, M S H; Backes, M; Schep, N W L; Ubbink, D T; Luitse, J S; Schepers, T; Goslings, J C

    2017-12-01

    Previous studies demonstrated that intra-operative fluoroscopic 3D-imaging (3D-imaging) in calcaneal fracture surgery is promising to prevent revision surgery and save costs. However, these studies limited their focus to corrections performed after 3D-imaging, thereby neglecting corrections after intra-operative fluoroscopic 2D-imaging (2D-imaging). The aim of this study was to assess the effects of additional 3D-imaging on intra-operative corrections, peri-operative imaging used, and patient-relevant outcomes compared to 2D-imaging alone. In this before-after study, data of adult patients who underwent open reduction and internal fixation (ORIF) of a calcaneal fracture between 2000 and 2014 in our level-I Trauma center were collected. 3D-imaging (BV Pulsera with 3D-RX, Philips Healthcare, Best, The Netherlands) was available as of 2007 at the surgeons' discretion. Patient and fracture characteristics, peri-operative imaging, intra-operative corrections and patient-relevant outcomes were collected from the hospital databases. Patients in whom additional 3D-imaging was applied were compared to those undergoing 2D-imaging alone. A total of 231 patients were included of whom 107 (46%) were operated with the use of 3D-imaging. No significant differences were found in baseline characteristics. The median duration of surgery was significantly longer when using 3D-imaging (2:08 vs. 1:54 h; p = 0.002). Corrections after additional 3D-imaging were performed in 53% of the patients. However, significantly fewer corrections were made after 2D-imaging when 3D-imaging was available (Risk difference (RD) -15%; 95% Confidence interval (CI) -29 to -2). Peri-operative imaging, besides intra-operative 3D-imaging, and patient-relevant outcomes were similar between groups. Intra-operative 3D-imaging provides additional information resulting in additional corrections. Moreover, 3D-imaging probably changed the surgeons' attitude to rely more on 3D-imaging, hence a 15%-decrease of

  8. GOTHIC CHURCHES IN PARIS ST GERVAIS ET ST PROTAIS IMAGE MATCHING 3D RECONSTRUCTION TO UNDERSTAND THE VAULTS SYSTEM GEOMETRY

    Directory of Open Access Journals (Sweden)

    M. Capone

    2015-02-01

    benefits and the troubles. From a methodological point of view this is our workflow: - theoretical study about geometrical configuration of rib vault systems; - 3D model based on theoretical hypothesis about geometric definition of the vaults' form; - 3D model based on image matching 3D reconstruction methods; - comparison between 3D theoretical model and 3D model based on image matching;

  9. 3D mutifractal analysis of cerebral tomoscintigraphy images

    International Nuclear Information System (INIS)

    Lopes, R.; Dubois, P.; Dewalle, A.S.; Betrouni, N.; Steinling, M.; Maouche, S.

    2007-01-01

    In this study, we describe the preliminary results of a tool to assist the diagnosis for the characterization of pathological cases of epilepsy disease using cerebral tomoscintigraphy images. The tool is based on the use of multifractal modelling to detect the local changes of homogeneity. (orig.)

  10. 3D microscopic imaging and evaluation of tubular tissue architecture

    Czech Academy of Sciences Publication Activity Database

    Janáček, Jiří; Čapek, Martin; Michálek, Jan; Karen, Petr; Kubínová, Lucie

    2014-01-01

    Roč. 63, Suppl.1 (2014), S49-S55 ISSN 0862-8408 R&D Projects: GA MŠk(CZ) LH13028; GA ČR(CZ) GA13-12412S Institutional support: RVO:67985823 Keywords : confocal microscopy * capillaries * brain * skeletal muscle * image analysis Subject RIV: EA - Cell Biology Impact factor: 1.293, year: 2014

  11. Revolving SEM images visualising 3D taxonomic characters

    DEFF Research Database (Denmark)

    Akkari, Nesrine; Cheung, David Koon-Bong; Enghoff, Henrik

    2013-01-01

    images taken consecutively while rotating the SEM stage 360°, which allows the structure in question to be seen from all angles of view in one plane. Seven new species of the genus Ommatoiulus collected in Tunisia are described: O. chambiensis, O. crassinigripes, O. kefi, O. khroumiriensis, O. xerophilus...

  12. Comparison of post-contrast 3D-T1-MPRAGE, 3D-T1-SPACE and 3D-T2-FLAIR MR images in evaluation of meningeal abnormalities at 3-T MRI.

    Science.gov (United States)

    Jeevanandham, Balaji; Kalyanpur, Tejas; Gupta, Prashant; Cherian, Mathew

    2017-06-01

    This study was to assess the usefulness of newer three-dimensional (3D)-T 1 sampling perfection with application optimized contrast using different flip-angle evolutions (SPACE) and 3D-T 2 fluid-attenuated inversion recovery (FLAIR) sequences in evaluation of meningeal abnormalities. 78 patients who presented with high suspicion of meningeal abnormalities were evaluated using post-contrast 3D-T 2 -FLAIR, 3D-T 1 magnetization-prepared rapid gradient-echo (MPRAGE) and 3D-T 1 -SPACE sequences. The images were evaluated independently by two radiologists for cortical gyral, sulcal space, basal cisterns and dural enhancement. The diagnoses were confirmed by further investigations including histopathology. Post-contrast 3D-T 1 -SPACE and 3D-T 2 -FLAIR images yielded significantly more information than MPRAGE images (p evaluation of meningeal abnormalities and when used in combination have the maximum sensitivity for leptomeningeal abnormalities. The negative-predictive value is nearly 100%, where no leptomeningeal abnormality was detected on these sequences. Advances in knowledge: Post-contrast 3D-T 1 -SPACE and 3D-T 2 -FLAIR images are more useful than 3D-T 1 -MPRAGE images in evaluation of meningeal abnormalities.

  13. Accuracy of 3D Imaging Software in Cephalometric Analysis

    Science.gov (United States)

    2013-06-21

    the accurate measurement of external apical root resorption (EARR). Lund, Grondahl and Grondahl (2010) reported that CBCT images of root length...H, Grondahl K, Grondahl HG. (2010). Cone beam computed tomography for assessment of root length and marginal bone level during orthodontic ... root angulation using panoramic and cone beam CT. Angle Orthodontics , 77(2), 206-213.   Periago DR, Scarge WC, Moshiri M, Scheetz JP, Silveira AM

  14. Current developments in clinical multiphoton tomography

    Science.gov (United States)

    König, Karsten; Weinigel, Martin; Breunig, Hans Georg; Gregory, Axel; Fischer, Peter; Kellner-Höfer, Marcel; Bückle, Rainer

    2010-02-01

    Two-photon microscopy has been introduced in 1990 [1]. 13 years later, CE-marked clinical multiphoton systems for 3D imaging of human skin with subcellular resolution have been launched by the JenLab company with the tomograph DermaInspectTM. In 2010, the second generation of clinical multiphoton tomographs was introduced. The novel mobile multiphoton tomograph MPTflexTM, equipped with a flexible articulated optical arm, provides an increased flexibility and accessibility especially for clinical and cosmetical examinations. The multiphoton excitation of fluorescent biomolecules like NAD(P)H, flavins, porphyrins, elastin, and melanin as well as the second harmonic generation of collagen is induced by picojoule femtosecond laser pulses from an tunable turn-key near infrared laser system. The ability for rapid highquality image acquisition, the user-friendly operation of the system, and the compact and flexible design qualifies this system to be used for melanoma detection, diagnostics of dermatological disorders, cosmetic research, and skin aging measurements as well as in situ drug monitoring and animal research. So far, more than 1,000 patients and volunteers have been investigated with the multiphoton tomographs in Europe, Asia, and Australia.

  15. Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy

    Science.gov (United States)

    Gualda, Emilio J.; Simão, Daniel; Pinto, Catarina; Alves, Paula M.; Brito, Catarina

    2014-01-01

    The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex 3D matrix, highly resembling physiological conditions. Light sheet fluorescence microscopy (LSFM) is becoming an excellent tool for fast imaging of such 3D biological structures. We demonstrate the potential of this technique for the imaging of human differentiated 3D neural aggregates in fixed and live samples, namely calcium imaging and cell death processes, showing the power of imaging modality compared with traditional microscopy. The combination of light sheet microscopy and 3D neural cultures will open the door to more challenging experiments involving drug testing at large scale as well as a better understanding of relevant biological processes in a more realistic environment. PMID:25161607

  16. 3D noise-resistant segmentation and tracking of unknown and occluded objects using integral imaging

    Science.gov (United States)

    Aloni, Doron; Jung, Jae-Hyun; Yitzhaky, Yitzhak

    2017-10-01

    Three dimensional (3D) object segmentation and tracking can be useful in various computer vision applications, such as: object surveillance for security uses, robot navigation, etc. We present a method for 3D multiple-object tracking using computational integral imaging, based on accurate 3D object segmentation. The method does not employ object detection by motion analysis in a video as conventionally performed (such as background subtraction or block matching). This means that the movement properties do not significantly affect the detection quality. The object detection is performed by analyzing static 3D image data obtained through computational integral imaging With regard to previous works that used integral imaging data in such a scenario, the proposed method performs the 3D tracking of objects without prior information about the objects in the scene, and it is found efficient under severe noise conditions.

  17. Remote laboratory for phase-aided 3D microscopic imaging and metrology

    Science.gov (United States)

    Wang, Meng; Yin, Yongkai; Liu, Zeyi; He, Wenqi; Li, Boqun; Peng, Xiang

    2014-05-01

    In this paper, the establishment of a remote laboratory for phase-aided 3D microscopic imaging and metrology is presented. Proposed remote laboratory consists of three major components, including the network-based infrastructure for remote control and data management, the identity verification scheme for user authentication and management, and the local experimental system for phase-aided 3D microscopic imaging and metrology. The virtual network computer (VNC) is introduced to remotely control the 3D microscopic imaging system. Data storage and management are handled through the open source project eSciDoc. Considering the security of remote laboratory, the fingerprint is used for authentication with an optical joint transform correlation (JTC) system. The phase-aided fringe projection 3D microscope (FP-3DM), which can be remotely controlled, is employed to achieve the 3D imaging and metrology of micro objects.

  18. AN IMAGE-BASED TECHNIQUE FOR 3D BUILDING RECONSTRUCTION USING MULTI-VIEW UAV IMAGES

    Directory of Open Access Journals (Sweden)

    F. Alidoost

    2015-12-01

    Full Text Available Nowadays, with the development of the urban areas, the automatic reconstruction of the buildings, as an important objects of the city complex structures, became a challenging topic in computer vision and photogrammetric researches. In this paper, the capability of multi-view Unmanned Aerial Vehicles (UAVs images is examined to provide a 3D model of complex building façades using an efficient image-based modelling workflow. The main steps of this work include: pose estimation, point cloud generation, and 3D modelling. After improving the initial values of interior and exterior parameters at first step, an efficient image matching technique such as Semi Global Matching (SGM is applied on UAV images and a dense point cloud is generated. Then, a mesh model of points is calculated using Delaunay 2.5D triangulation and refined to obtain an accurate model of building. Finally, a texture is assigned to mesh in order to create a realistic 3D model. The resulting model has provided enough details of building based on visual assessment.

  19. Detection of tibial condylar fractures using 3D imaging with a mobile image amplifier (Siemens ISO-C-3D): Comparison with plain films and spiral CT

    International Nuclear Information System (INIS)

    Kotsianos, D.; Rock, C.; Wirth, S.; Linsenmaier, U.; Brandl, R.; Fischer, T.; Pfeifer, K.J.; Reiser, M.; Euler, E.; Mutschler, W.

    2002-01-01

    Purpose: To analyze a prototype mobile C-arm 3D image amplifier in the detection and classification of experimental tibial condylar fractures with multiplanar reconstructions (MPR). Method: Human knee specimens (n=22) with tibial condylar fractures were examined with a prototype C-arm (ISO-C-3D, Siemens AG), plain films (CR) and spiral CT (CT). The motorized C-arm provides fluoroscopic images during a 190 orbital rotation computing a 119 mm data cube. From these 3D data sets MP reconstructions were obtained. All images were evaluated by four independent readers for the detection and assessment of fracture lines. All fractures were classified according to the Mueller AO classification. To confirm the results, the specimens were finally surgically dissected. Results: 97% of the tibial condylar fractures were easily seen and correctly classified according to the Mueller AO classification on MP reconstruction of the ISO-C-3D. There is no significant difference between ISO-C and CT in detection and correct classification of fractures, but ISO-CD-3D is significant by better than CR. (orig.) [de

  20. Multithreaded real-time 3D image processing software architecture and implementation

    Science.gov (United States)

    Ramachandra, Vikas; Atanassov, Kalin; Aleksic, Milivoje; Goma, Sergio R.

    2011-03-01

    Recently, 3D displays and videos have generated a lot of interest in the consumer electronics industry. To make 3D capture and playback popular and practical, a user friendly playback interface is desirable. Towards this end, we built a real time software 3D video player. The 3D video player displays user captured 3D videos, provides for various 3D specific image processing functions and ensures a pleasant viewing experience. Moreover, the player enables user interactivity by providing digital zoom and pan functionalities. This real time 3D player was implemented on the GPU using CUDA and OpenGL. The player provides user interactive 3D video playback. Stereo images are first read by the player from a fast drive and rectified. Further processing of the images determines the optimal convergence point in the 3D scene to reduce eye strain. The rationale for this convergence point selection takes into account scene depth and display geometry. The first step in this processing chain is identifying keypoints by detecting vertical edges within the left image. Regions surrounding reliable keypoints are then located on the right image through the use of block matching. The difference in the positions between the corresponding regions in the left and right images are then used to calculate disparity. The extrema of the disparity histogram gives the scene disparity range. The left and right images are shifted based upon the calculated range, in order to place the desired region of the 3D scene at convergence. All the above computations are performed on one CPU thread which calls CUDA functions. Image upsampling and shifting is performed in response to user zoom and pan. The player also consists of a CPU display thread, which uses OpenGL rendering (quad buffers). This also gathers user input for digital zoom and pan and sends them to the processing thread.

  1. 3D-CT imaging processing for qualitative and quantitative analysis of maxillofacial cysts and tumors

    International Nuclear Information System (INIS)

    Cavalcanti, Marcelo de Gusmao Paraiso; Antunes, Jose Leopoldo Ferreira

    2002-01-01

    The objective of this study was to evaluate spiral-computed tomography (3D-CT) images of 20 patients presenting with cysts and tumors in the maxillofacial complex, in order to compare the surface and volume techniques of image rendering. The qualitative and quantitative appraisal indicated that the volume technique allowed a more precise and accurate observation than the surface method. On the average, the measurements obtained by means of the 3D volume-rendering technique were 6.28% higher than those obtained by means of the surface method. The sensitivity of the 3D surface technique was lower than that of the 3D volume technique for all conditions stipulated in the diagnosis and evaluation of lesions. We concluded that the 3D-CT volume rendering technique was more reproducible and sensitive than the 3D-CT surface method, in the diagnosis, treatment planning and evaluation of maxillofacial lesions, especially those with intra-osseous involvement. (author)

  2. 3D-CT imaging processing for qualitative and quantitative analysis of maxillofacial cysts and tumors

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcanti, Marcelo de Gusmao Paraiso [Sao Paulo Univ., SP (Brazil). Faculdade de Odontologia. Dept. de Radiologia; Antunes, Jose Leopoldo Ferreira [Sao Paulo Univ., SP (Brazil). Faculdade de Odotologia. Dept. de Odontologia Social

    2002-09-01

    The objective of this study was to evaluate spiral-computed tomography (3D-CT) images of 20 patients presenting with cysts and tumors in the maxillofacial complex, in order to compare the surface and volume techniques of image rendering. The qualitative and quantitative appraisal indicated that the volume technique allowed a more precise and accurate observation than the surface method. On the average, the measurements obtained by means of the 3D volume-rendering technique were 6.28% higher than those obtained by means of the surface method. The sensitivity of the 3D surface technique was lower than that of the 3D volume technique for all conditions stipulated in the diagnosis and evaluation of lesions. We concluded that the 3D-CT volume rendering technique was more reproducible and sensitive than the 3D-CT surface method, in the diagnosis, treatment planning and evaluation of maxillofacial lesions, especially those with intra-osseous involvement. (author)

  3. 3D Modeling from Multi-views Images for Cultural Heritage in Wat-Pho, Thailand

    Science.gov (United States)

    Soontranon, N.; Srestasathiern, P.; Lawawirojwong, S.

    2015-08-01

    In Thailand, there are several types of (tangible) cultural heritages. This work focuses on 3D modeling of the heritage objects from multi-views images. The images are acquired by using a DSLR camera which costs around 1,500 (camera and lens). Comparing with a 3D laser scanner, the camera is cheaper and lighter than the 3D scanner. Hence, the camera is available for public users and convenient for accessing narrow areas. The acquired images consist of various sculptures and architectures in Wat-Pho which is a Buddhist temple located behind the Grand Palace (Bangkok, Thailand). Wat-Pho is known as temple of the reclining Buddha and the birthplace of traditional Thai massage. To compute the 3D models, a diagram is separated into following steps; Data acquisition, Image matching, Image calibration and orientation, Dense matching and Point cloud processing. For the initial work, small heritages less than 3 meters height are considered for the experimental results. A set of multi-views images of an interested object is used as input data for 3D modeling. In our experiments, 3D models are obtained from MICMAC (open source) software developed by IGN, France. The output of 3D models will be represented by using standard formats of 3D point clouds and triangulated surfaces such as .ply, .off, .obj, etc. To compute for the efficient 3D models, post-processing techniques are required for the final results e.g. noise reduction, surface simplification and reconstruction. The reconstructed 3D models can be provided for public access such as website, DVD, printed materials. The high accurate 3D models can also be used as reference data of the heritage objects that must be restored due to deterioration of a lifetime, natural disasters, etc.

  4. Anesthesiology training using 3D imaging and virtual reality

    Science.gov (United States)

    Blezek, Daniel J.; Robb, Richard A.; Camp, Jon J.; Nauss, Lee A.

    1996-04-01

    Current training for regional nerve block procedures by anesthesiology residents requires expert supervision and the use of cadavers; both of which are relatively expensive commodities in today's cost-conscious medical environment. We are developing methods to augment and eventually replace these training procedures with real-time and realistic computer visualizations and manipulations of the anatomical structures involved in anesthesiology procedures, such as nerve plexus injections (e.g., celiac blocks). The initial work is focused on visualizations: both static images and rotational renderings. From the initial results, a coherent paradigm for virtual patient and scene representation will be developed.

  5. Value of 3-dimensional (3D) imaging in rheumatology

    International Nuclear Information System (INIS)

    Fredy, D.

    1990-01-01

    The whole body scanner (Exel 2.400) of the Centre Hospitalier Sainte-Anne enables the three-dimensional reconstruction, with visualization, of the object in its real volume in less than 10 minutes after taking 20 to 40 radiological sections. The exploration can be complete at all levels. Bone lesions can be perfectly shown, the study of osteoarticular or intraspinal abnormalities is facilitated, all solution of continuity can be detected. A soft parts program as well as a colour program enable a clear and rapid visualization of organic lesions. Three-dimensional imaging can be of great value in rheumatology [fr

  6. 3D Tomographic Image Reconstruction using CUDA C

    International Nuclear Information System (INIS)

    Dominguez, J. S.; Assis, J. T.; Oliveira, L. F. de

    2011-01-01

    This paper presents the study and implementation of a software for three dimensional reconstruction of images obtained with a tomographic system using the capabilities of Graphic Processing Units(GPU). The reconstruction by filtered back-projection method was developed using the CUDA C, for maximum utilization of the processing capabilities of GPUs to solve computational problems with large computational cost and highly parallelizable. It was discussed the potential of GPUs and shown its advantages to solving this kind of problems. The results in terms of runtime will be compared with non-parallelized implementations and must show a great reduction of processing time. (Author)

  7. Synthetic Microwave Imaging Reflectometry diagnostic using 3D FDTD Simulations

    Science.gov (United States)

    Kruger, Scott; Jenkins, Thomas; Smithe, David; King, Jacob; Nimrod Team Team

    2017-10-01

    Microwave Imaging Reflectometry (MIR) has become a standard diagnostic for understanding tokamak edge perturbations, including the edge harmonic oscillations in QH mode operation. These long-wavelength perturbations are larger than the normal turbulent fluctuation levels and thus normal analysis of synthetic signals become more difficult. To investigate, we construct a synthetic MIR diagnostic for exploring density fluctuation amplitudes in the tokamak plasma edge by using the three-dimensional, full-wave FDTD code Vorpal. The source microwave beam for the diagnostic is generated and refelected at the cutoff surface that is distorted by 2D density fluctuations in the edge plasma. Synthetic imaging optics at the detector can be used to understand the fluctuation and background density profiles. We apply the diagnostic to understand the fluctuations in edge plasma density during QH-mode activity in the DIII-D tokamak, as modeled by the NIMROD code. This work was funded under DOE Grant Number DE-FC02-08ER54972.

  8. Parallel 3-D image processing for nuclear emulsion

    International Nuclear Information System (INIS)

    Nakano, Toshiyuki

    2001-01-01

    The history of nuclear plate was explained. The first nuclear plate was named as pellicles covered with 600 μm of emulsion in Europe. In Japan Emulsion Cloud Chamber (ECC) using thin emulsion (50 μm) type nuclear plate was developed in 1960. Then, the semi-automatic analyzer (1971) and automatic analyzer (1980), Track Selector (TS) with memory stored 16 layer images in 512 x 512 x 16 pixel were developed. Moreover, NTS (New Track Selector), speeding up analyzer, was produced for analysis of results of CHORUS experiment in 1996. Simultaneous readout of 16 layer images had been carried out, but UTS (Ultra Track Selector) made possible to progressive treatment of 16 layers of some data and determination of traces in all angles. Direct detection of tau neutrino (VT) was studied by DONUT (FNAL E872) using UTS and nuclear plate. Neutrino beam was produced by 800 GeV proton beam hitting the fixed target. About 1100 phenomena of neutrino reactions were observed during six months of irradiation. 203 phenomena were detected. 4 examples were shown in this paper. OPERA experiment by SK is explained. (S.Y.)

  9. A new approach towards image based virtual 3D city modeling by using close range photogrammetry

    Science.gov (United States)

    Singh, S. P.; Jain, K.; Mandla, V. R.

    2014-05-01

    3D city model is a digital representation of the Earth's surface and it's related objects such as building, tree, vegetation, and some manmade feature belonging to urban area. The demand of 3D city modeling is increasing day to day for various engineering and non-engineering applications. Generally three main image based approaches are using for virtual 3D city models generation. In first approach, researchers used Sketch based modeling, second method is Procedural grammar based modeling and third approach is Close range photogrammetry based modeling. Literature study shows that till date, there is no complete solution available to create complete 3D city model by using images. These image based methods also have limitations This paper gives a new approach towards image based virtual 3D city modeling by using close range photogrammetry. This approach is divided into three sections. First, data acquisition process, second is 3D data processing, and third is data combination process. In data acquisition process, a multi-camera setup developed and used for video recording of an area. Image frames created from video data. Minimum required and suitable video image frame selected for 3D processing. In second section, based on close range photogrammetric principles and computer vision techniques, 3D model of area created. In third section, this 3D model exported to adding and merging of other pieces of large area. Scaling and alignment of 3D model was done. After applying the texturing and rendering on this model, a final photo-realistic textured 3D model created. This 3D model transferred into walk-through model or in movie form. Most of the processing steps are automatic. So this method is cost effective and less laborious. Accuracy of this model is good. For this research work, study area is the campus of department of civil engineering, Indian Institute of Technology, Roorkee. This campus acts as a prototype for city. Aerial photography is restricted in many country

  10. DEM GENERATION FROM HIGH RESOLUTION SATELLITE IMAGES THROUGH A NEW 3D LEAST SQUARES MATCHING ALGORITHM

    Directory of Open Access Journals (Sweden)

    T. Kim

    2012-09-01

    Full Text Available Automated generation of digital elevation models (DEMs from high resolution satellite images (HRSIs has been an active research topic for many years. However, stereo matching of HRSIs, in particular based on image-space search, is still difficult due to occlusions and building facades within them. Object-space matching schemes, proposed to overcome these problem, often are very time consuming and critical to the dimensions of voxels. In this paper, we tried a new least square matching (LSM algorithm that works in a 3D object space. The algorithm starts with an initial height value on one location of the object space. From this 3D point, the left and right image points are projected. The true height is calculated by iterative least squares estimation based on the grey level differences between the left and right patches centred on the projected left and right points. We tested the 3D LSM to the Worldview images over 'Terrassa Sud' provided by the ISPRS WG I/4. We also compared the performance of the 3D LSM with the correlation matching based on 2D image space and the correlation matching based on 3D object space. The accuracy of the DEM from each method was analysed against the ground truth. Test results showed that 3D LSM offers more accurate DEMs over the conventional matching algorithms. Results also showed that 3D LSM is sensitive to the accuracy of initial height value to start the estimation. We combined the 3D COM and 3D LSM for accurate and robust DEM generation from HRSIs. The major contribution of this paper is that we proposed and validated that LSM can be applied to object space and that the combination of 3D correlation and 3D LSM can be a good solution for automated DEM generation from HRSIs.

  11. Potential Cost Savings for Use of 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization

    Science.gov (United States)

    2014-04-30

    bäÉîÉåíÜ=^ååì~ä=^Åèìáëáíáçå= oÉëÉ~êÅÜ=póãéçëáìã= qÜìêëÇ~ó=pÉëëáçåë= sçäìãÉ=ff= = Potential Cost Savings for Use of 3D Printing Combined With 3D...TYPE 3. DATES COVERED 00-00-2014 to 00-00-2014 4. TITLE AND SUBTITLE Potential Cost Savings for Use of 3D Printing Combined With 3D Imaging and...Chair: RADM David Lewis, USN Program Executive Officer, SHIPS Potential Cost Savings for Use of 3D Printing Combined With 3D Imaging and CPLM for

  12. Technology Development for 3-D Wide Swath Imaging Supporting ACE

    Science.gov (United States)

    Racette, Paul; Heymsfield, Gerry; Li, Lihua; Mclinden, Matthew; Park, Richard; Cooley, Michael; Stenger, Pete; Hand, Thomas

    2014-01-01

    The National Academy of Sciences Decadal Survey (DS) Aerosol-Cloud-Ecosystems Mission (ACE) aims to advance our ability to observe and predict changes to the Earth's hydrological cycle and energy balance in response to climate forcing, especially those changes associated with the effects of aerosol on clouds and precipitation. ACE is focused on obtaining measurements to reduce the uncertainties in current climate models arising from the lack in understanding of aerosol-cloud interactions. As part of the mission instrument suite, a dual-frequency radar comprised of a fixed-beam 94 gigahertz (W-band) radar and a wide-swath 35 gigahertz (Ka-band) imaging radar has been recommended by the ACE Science Working Group.In our 2010 Instrument Incubator Program project, we've developed a radar architecture that addresses the challenge associated with achieving the measurement objectives through an innovative, shared aperture antenna that allows dual-frequency radar operation while achieving wide-swath (100 kilometers) imaging at Ka-band. The antenna system incorporates 2 key technologies; a) a novel dual-band reflectorreflectarray and b) a Ka-band Active Electronically Scanned Array (AESA) feed module. The dual-band antenna is comprised of a primary cylindrical reflectorreflectarray surface illuminated by a point-focus W-band feed (compatible with a quasi-optical beam waveguide feed, such as that employed on CloudSat); the Ka-band AESA line feed provides wide-swath across-track scanning. The benefits of this shared-aperture approach include significant reductions in ACE satellite payload size, weight, and cost, as compared to a two aperture approach. Four objectives were addressed in our project. The first entailed developing the tools for the analysis and design of reflectarray antennas, assessment of candidate reflectarray elements, and validation using test coupons. The second objective was to develop a full-scale aperture design utilizing the reflectarray surface and to

  13. Triangular SPECT system for 3-D total organ volume imaging: Design concept and preliminary imaging results

    International Nuclear Information System (INIS)

    Lim, C.B.; Anderson, J.; Covic, J.

    1985-01-01

    SPECT systems based on 2-D detectors for projection data collection and filtered back-projection image reconstruction have the potential for true 3-D imaging, providing contiguous slice images in any orientation. Anger camera-based SPECT systems have the natural advantage supporting planar imaging clinical procedures. However, current systems suffer from two drawbacks; poor utilization of emitted photons, and inadequate system design for SPECT. A SPECT system consisting of three rectangular cameras with radial translation would offer the variable cylindrical FOV of 25 cm to 40 cm diameter allowing close detector access to the object. This system would provide optimized imaging for both brain and body organs in terms of sensitivity and resolution. For brain imaging a tight detector triangle with fan beam collimation, matching detector UFOV to the head, allows full 2 π utilization of emitted photons, resulting in >4 times sensitivity increase over the single detector system. Minification of intrinsic detector resolution in fan beam collimation further improves system resolution. For body organ imaging the three detectors with parallel hole collimators, rotating in non-circular orbit, provide both improved resolution and three-fold sensitivity increase. Practical challenges lie in ensuring perfect image overlap from three detectors without resolution degradation and artifact generation in order to benefit from the above improvements. An experimental system has been developed to test the above imaging concept and we have successfully demonstrated the superior image quality of the overlapped images. Design concept will be presented with preliminary imaging results

  14. Intensity-based segmentation and visualization of cells in 3D microscopic images using the GPU

    Science.gov (United States)

    Kang, Mi-Sun; Lee, Jeong-Eom; Jeon, Woong-ki; Choi, Heung-Kook; Kim, Myoung-Hee

    2013-02-01

    3D microscopy images contain abundant astronomical data, rendering 3D microscopy image processing time-consuming and laborious on a central processing unit (CPU). To solve these problems, many people crop a region of interest (ROI) of the input image to a small size. Although this reduces cost and time, there are drawbacks at the image processing level, e.g., the selected ROI strongly depends on the user and there is a loss in original image information. To mitigate these problems, we developed a 3D microscopy image processing tool on a graphics processing unit (GPU). Our tool provides efficient and various automatic thresholding methods to achieve intensity-based segmentation of 3D microscopy images. Users can select the algorithm to be applied. Further, the image processing tool provides visualization of segmented volume data and can set the scale, transportation, etc. using a keyboard and mouse. However, the 3D objects visualized fast still need to be analyzed to obtain information for biologists. To analyze 3D microscopic images, we need quantitative data of the images. Therefore, we label the segmented 3D objects within all 3D microscopic images and obtain quantitative information on each labeled object. This information can use the classification feature. A user can select the object to be analyzed. Our tool allows the selected object to be displayed on a new window, and hence, more details of the object can be observed. Finally, we validate the effectiveness of our tool by comparing the CPU and GPU processing times by matching the specification and configuration.

  15. Imaging multipole gravity anomaly sources by 3D probability tomography

    International Nuclear Information System (INIS)

    Alaia, Raffaele; Patella, Domenico; Mauriello, Paolo

    2009-01-01

    We present a generalized theory of the probability tomography applied to the gravity method, assuming that any Bouguer anomaly data set can be caused by a discrete number of monopoles, dipoles, quadrupoles and octopoles. These elementary sources are used to characterize, in an as detailed as possible way and without any a priori assumption, the shape and position of the most probable minimum structure of the gravity sources compatible with the observed data set, by picking out the location of their centres and peculiar points of their boundaries related to faces, edges and vertices. A few synthetic examples using simple geometries are discussed in order to demonstrate the notably enhanced resolution power of the new approach, compared with a previous formulation that used only monopoles and dipoles. A field example related to a gravity survey carried out in the volcanic area of Mount Etna (Sicily, Italy) is presented, aimed at imaging the geometry of the minimum gravity structure down to 8 km of depth bsl

  16. Algorithm of pulmonary emphysema extraction using low dose thoracic 3D CT images

    Science.gov (United States)

    Saita, S.; Kubo, M.; Kawata, Y.; Niki, N.; Nakano, Y.; Omatsu, H.; Tominaga, K.; Eguchi, K.; Moriyama, N.

    2006-03-01

    Recently, due to aging and smoking, emphysema patients are increasing. The restoration of alveolus which was destroyed by emphysema is not possible, thus early detection of emphysema is desired. We describe a quantitative algorithm for extracting emphysematous lesions and quantitatively evaluate their distribution patterns using low dose thoracic 3-D CT images. The algorithm identified lung anatomies, and extracted low attenuation area (LAA) as emphysematous lesion candidates. Applying the algorithm to 100 thoracic 3-D CT images and then by follow-up 3-D CT images, we demonstrate its potential effectiveness to assist radiologists and physicians to quantitatively evaluate the emphysematous lesions distribution and their evolution in time interval changes.

  17. Algorithm of pulmonary emphysema extraction using thoracic 3D CT images

    Science.gov (United States)

    Saita, Shinsuke; Kubo, Mitsuru; Kawata, Yoshiki; Niki, Noboru; Nakano, Yasutaka; Ohmatsu, Hironobu; Tominaga, Keigo; Eguchi, Kenji; Moriyama, Noriyuki

    2007-03-01

    Recently, due to aging and smoking, emphysema patients are increasing. The restoration of alveolus which was destroyed by emphysema is not possible, thus early detection of emphysema is desired. We describe a quantitative algorithm for extracting emphysematous lesions and quantitatively evaluate their distribution patterns using low dose thoracic 3-D CT images. The algorithm identified lung anatomies, and extracted low attenuation area (LAA) as emphysematous lesion candidates. Applying the algorithm to thoracic 3-D CT images and then by follow-up 3-D CT images, we demonstrate its potential effectiveness to assist radiologists and physicians to quantitatively evaluate the emphysematous lesions distribution and their evolution in time interval changes.

  18. Soft tissue segmentation and 3D display from computerized tomography and magnetic resonance imaging

    International Nuclear Information System (INIS)

    Fan, R.T.; Trivedi, S.S.; Fellingham, L.L.; Gamboa-Aldeco, A.; Hedgcock, M.W.

    1987-01-01

    Volume calculation and 3D display of human anatomy facilitate a physician's diagnosis, treatment, and evaluation. Accurate segmentation of soft tissue structures is a prerequisite for such volume calculations and 3D displays, but segmentation by hand-outlining structures is often tedious and time-consuming. In this paper, methods based on analysis of statistics of image gray level are applied to segmentation of soft tissue in medical images, with the goal of making segmentation automatic or semi-automatic. The resulting segmented images, volume calculations, and 3D displays are analyzed and compared with results based on physician-drawn outlines as well as actual volume measurements

  19. A simple device for the stereoscopic display of 3D CT images

    International Nuclear Information System (INIS)

    Haveri, M.; Suramo, I.; Laehde, S.; Karhula, V.; Junila, J.

    1997-01-01

    We describe a simple device for creating true 3D views of image pairs obtained at 3D CT reconstruction. The device presents the images in a slightly different angle of view for the left and the right eyes. This true 3D viewing technique was applied experimentally in the evaluation of complex acetabular fractures. Experiments were also made to determine the optimal angle between the images for each eye. The angle varied between 1 and 7 for different observers and also depended on the display field of view used. (orig.)

  20. Embedded, real-time UAV control for improved, image-based 3D scene reconstruction

    Science.gov (United States)

    Jean Liénard; Andre Vogs; Demetrios Gatziolis; Nikolay Strigul

    2016-01-01

    Unmanned Aerial Vehicles (UAVs) are already broadly employed for 3D modeling of large objects such as trees and monuments via photogrammetry. The usual workflow includes two distinct steps: image acquisition with UAV and computationally demanding postflight image processing. Insufficient feature overlaps across images is a common shortcoming in post-flight image...

  1. Mutual information as a measure of image quality for 3D dynamic lung imaging with EIT.

    Science.gov (United States)

    Crabb, M G; Davidson, J L; Little, R; Wright, P; Morgan, A R; Miller, C A; Naish, J H; Parker, G J M; Kikinis, R; McCann, H; Lionheart, W R B

    2014-05-01

    We report on a pilot study of dynamic lung electrical impedance tomography (EIT) at the University of Manchester. Low-noise EIT data at 100 frames per second were obtained from healthy male subjects during controlled breathing, followed by magnetic resonance imaging (MRI) subsequently used for spatial validation of the EIT reconstruction. The torso surface in the MR image and electrode positions obtained using MRI fiducial markers informed the construction of a 3D finite element model extruded along the caudal-distal axis of the subject. Small changes in the boundary that occur during respiration were accounted for by incorporating the sensitivity with respect to boundary shape into a robust temporal difference reconstruction algorithm. EIT and MRI images were co-registered using the open source medical imaging software, 3D Slicer. A quantitative comparison of quality of different EIT reconstructions was achieved through calculation of the mutual information with a lung-segmented MR image. EIT reconstructions using a linear shape correction algorithm reduced boundary image artefacts, yielding better contrast of the lungs, and had 10% greater mutual information compared with a standard linear EIT reconstruction.

  2. MR Imaging of the Internal Auditory Canal and Inner Ear at 3T: Comparison between 3D Driven Equilibrium and 3D Balanced Fast Field Echo Sequences

    Energy Technology Data Exchange (ETDEWEB)

    Byun, Jun Soo; Kim, Hyung Jin; Yim, Yoo Jeong; Kim, Sung Tae; Jeon, Pyoung; Kim, Keon Ha [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of); Kim, Sam Soo; Jeon, Yong Hwan; Lee, Ji Won [Kangwon National University College of Medicine, Chuncheon (Korea, Republic of)

    2008-06-15

    To compare the use of 3D driven equilibrium (DRIVE) imaging with 3D balanced fast field echo (bFFE) imaging in the assessment of the anatomic structures of the internal auditory canal (IAC) and inner ear at 3 Tesla (T). Thirty ears of 15 subjects (7 men and 8 women; age range, 22 71 years; average age, 50 years) without evidence of ear problems were examined on a whole-body 3T MR scanner with both 3D DRIVE and 3D bFFE sequences by using an 8-channel sensitivity encoding (SENSE) head coil. Two neuroradiologists reviewed both MR images with particular attention to the visibility of the anatomic structures, including four branches of the cranial nerves within the IAC, anatomic structures of the cochlea, vestibule, and three semicircular canals. Although both techniques provided images of relatively good quality, the 3D DRIVE sequence was somewhat superior to the 3D bFFE sequence. The discrepancies were more prominent for the basal turn of the cochlea, vestibule, and all semicircular canals, and were thought to be attributed to the presence of greater magnetic susceptibility artifacts inherent to gradient-echo techniques such as bFFE. Because of higher image quality and less susceptibility artifacts, we highly recommend the employment of 3D DRIVE imaging as the MR imaging choice for the IAC and inner ear

  3. 3D-TV System with Depth-Image-Based Rendering Architectures, Techniques and Challenges

    CERN Document Server

    Zhao, Yin; Yu, Lu; Tanimoto, Masayuki

    2013-01-01

    Riding on the success of 3D cinema blockbusters and advances in stereoscopic display technology, 3D video applications have gathered momentum in recent years. 3D-TV System with Depth-Image-Based Rendering: Architectures, Techniques and Challenges surveys depth-image-based 3D-TV systems, which are expected to be put into applications in the near future. Depth-image-based rendering (DIBR) significantly enhances the 3D visual experience compared to stereoscopic systems currently in use. DIBR techniques make it possible to generate additional viewpoints using 3D warping techniques to adjust the perceived depth of stereoscopic videos and provide for auto-stereoscopic displays that do not require glasses for viewing the 3D image.   The material includes a technical review and literature survey of components and complete systems, solutions for technical issues, and implementation of prototypes. The book is organized into four sections: System Overview, Content Generation, Data Compression and Transmission, and 3D V...

  4. A framework for human spine imaging using a freehand 3D ultrasound system

    NARCIS (Netherlands)

    Purnama, Ketut E.; Wilkinson, Michael H.F.; Veldhuizen, Albert G.; van Ooijen, Peter M.A.; Lubbers, Jaap; Burgerhof, Johannes G.M.; Sardjono, Tri A.; Verkerke, Gijsbertus Jacob

    2010-01-01

    The use of 3D ultrasound imaging to follow the progression of scoliosis, i.e., a 3D deformation of the spine, is described. Unlike other current examination modalities, in particular based on X-ray, its non-detrimental effect enables it to be used frequently to follow the progression of scoliosis

  5. Contributions in compression of 3D medical images and 2D images

    International Nuclear Information System (INIS)

    Gaudeau, Y.

    2006-12-01

    The huge amounts of volumetric data generated by current medical imaging techniques in the context of an increasing demand for long term archiving solutions, as well as the rapid development of distant radiology make the use of compression inevitable. Indeed, if the medical community has sided until now with compression without losses, most of applications suffer from compression ratios which are too low with this kind of compression. In this context, compression with acceptable losses could be the most appropriate answer. So, we propose a new loss coding scheme based on 3D (3 dimensional) Wavelet Transform and Dead Zone Lattice Vector Quantization 3D (DZLVQ) for medical images. Our algorithm has been evaluated on several computerized tomography (CT) and magnetic resonance image volumes. The main contribution of this work is the design of a multidimensional dead zone which enables to take into account correlations between neighbouring elementary volumes. At high compression ratios, we show that it can out-perform visually and numerically the best existing methods. These promising results are confirmed on head CT by two medical patricians. The second contribution of this document assesses the effect with-loss image compression on CAD (Computer-Aided Decision) detection performance of solid lung nodules. This work on 120 significant lungs images shows that detection did not suffer until 48:1 compression and still was robust at 96:1. The last contribution consists in the complexity reduction of our compression scheme. The first allocation dedicated to 2D DZLVQ uses an exponential of the rate-distortion (R-D) functions. The second allocation for 2D and 3D medical images is based on block statistical model to estimate the R-D curves. These R-D models are based on the joint distribution of wavelet vectors using a multidimensional mixture of generalized Gaussian (MMGG) densities. (author)

  6. Multiphoton microscopy based cryo-imaging of inflated frozen human lung sections at -60°C in healthy and COPD lungs

    Science.gov (United States)

    Abraham, Thomas; Kayra, Damian; Zhang, Angela; Suzuki, Masaru; McDonough, John; Elliott, W. M.; Cooper, Joel D.; Hogg, James C.

    2013-02-01

    Lung is a complex gas exchanger with interfacial area (where the gas exchange takes place) is about the size of a tennis court. Respiratory function is linked to the biomechanical stability of the gas exchange or alveolar regions which directly depends on the spatial distributions of the extracellular matrix fibers such fibrillar collagens and elastin fibers. It is very important to visualize and quantify these fibers at their native and inflated conditions to have correct morphometric information on differences between control and diseased states. This can be only achieved in the ex vivo states by imaging directly frozen lung specimens inflated to total lung capacity. Multiphoton microscopy, which uses ultra-short infrared laser pulses as the excitation source, produces multiphoton excitation fluorescence (MPEF) signals from endogenously fluorescent proteins (e.g. elastin) and induces specific second harmonic generation (SHG) signals from non-centrosymmetric proteins such as fibrillar collagens in fresh human lung tissues [J. Struct. Biol. (2010)171,189-196]. Here we report for the first time 3D image data obtained directly from thick frozen inflated lung specimens (~0.7- 1.0 millimeter thick) visualized at -60°C without prior fixation or staining in healthy and diseased states. Lung specimens donated for transplantation and released for research when no appropriate recipient was identified served as controls, and diseased lung specimens donated for research by patients receiving lung transplantation for very severe COPD (n=4) were prepared as previously described [N. Engl. J. Med. (2011) 201, 1567]. Lung slices evenly spaced between apex and base were examined using multiphoton microscopy while maintained at -60°C using a temperature controlled cold stage with a temperature resolution of 0.1°C. Infrared femto-second laser pulses tuned to 880nm, dry microscopic objectives, and non-de-scanned detectors/spectrophotometer located in the reflection geometry were

  7. Depiction of the cranial nerves around the cavernous sinus by 3D reversed FISP with diffusion weighted imaging (3D PSIF-DWI)

    International Nuclear Information System (INIS)

    Ishida, Go; Oishi, Makoto; Jinguji, Shinya; Yoneoka, Yuichiro; Fujii, Yukihiko; Sato, Mitsuya

    2011-01-01

    The purpose of this study was to evaluate the anatomy of cranial nerves running in and around the cavernous sinus, we employed three-dimensional reversed fast imaging with steady-state precession (FISP) with diffusion weighted imaging (3D PSIF-DWI) on 3-T magnetic resonance (MR) system. After determining the proper parameters to obtain sufficient resolution of 3D PSIF-DWI, we collected imaging data of 20-side cavernous regions in 10 normal subjects. 3D PSIF-DWI provided high contrast between the cranial nerves and other soft tissues, fluid, and blood in all subjects. We also created volume-rendered images of 3D PSIF-DWI and anatomically evaluated the reliability of visualizing optic, oculomotor, trochlear, trigeminal, and abducens nerves on 3D PSIF-DWI. All 20 sets of cranial nerves were visualized and 12 trochlear nerves and 6 abducens nerves were partially identified. We also presented preliminary clinical experiences in two cases with pituitary adenomas. The anatomical relationship between the tumor and cranial nerves running in and around the cavernous sinus could be three-dimensionally comprehended by 3D PSIF-DWI and the volume-rendered images. In conclusion, 3D PSIF-DWI has great potential to provide high resolution 'cranial nerve imaging', which visualizes the whole length of the cranial nerves including the parts in the blood flow as in the cavernous sinus region. (author)

  8. [Depiction of the cranial nerves around the cavernous sinus by 3D reversed FISP with diffusion weighted imaging (3D PSIF-DWI)].

    Science.gov (United States)

    Ishida, Go; Oishi, Makoto; Jinguji, Shinya; Yoneoka, Yuichiro; Sato, Mitsuya; Fujii, Yukihiko

    2011-10-01

    To evaluate the anatomy of cranial nerves running in and around the cavernous sinus, we employed three-dimensional reversed fast imaging with steady-state precession (FISP) with diffusion weighted imaging (3D PSIF-DWI) on 3-T magnetic resonance (MR) system. After determining the proper parameters to obtain sufficient resolution of 3D PSIF-DWI, we collected imaging data of 20-side cavernous regions in 10 normal subjects. 3D PSIF-DWI provided high contrast between the cranial nerves and other soft tissues, fluid, and blood in all subjects. We also created volume-rendered images of 3D PSIF-DWI and anatomically evaluated the reliability of visualizing optic, oculomotor, trochlear, trigeminal, and abducens nerves on 3D PSIF-DWI. All 20 sets of cranial nerves were visualized and 12 trochlear nerves and 6 abducens nerves were partially identified. We also presented preliminary clinical experiences in two cases with pituitary adenomas. The anatomical relationship between the tumor and cranial nerves running in and around the cavernous sinus could be three-dimensionally comprehended by 3D PSIF-DWI and the volume-rendered images. In conclusion, 3D PSIF-DWI has great potential to provide high resolution "cranial nerve imaging", which visualizes the whole length of the cranial nerves including the parts in the blood flow as in the cavernous sinus region.

  9. 3D printing of intracranial artery stenosis based on the source images of magnetic resonance angiograph.

    Science.gov (United States)

    Xu, Wei-Hai; Liu, Jia; Li, Ming-Li; Sun, Zhao-Yong; Chen, Jie; Wu, Jian-Huang

    2014-08-01

    Three dimensional (3D) printing techniques for brain diseases have not been widely studied. We attempted to 'print' the segments of intracranial arteries based on magnetic resonance imaging. Three dimensional magnetic resonance angiography (MRA) was performed on two patients with middle cerebral artery (MCA) stenosis. Using scale-adaptive vascular modeling, 3D vascular models were constructed from the MRA source images. The magnified (ten times) regions of interest (ROI) of the stenotic segments were selected and fabricated by a 3D printer with a resolution of 30 µm. A survey to 8 clinicians was performed to evaluate the accuracy of 3D printing results as compared with MRA findings (4 grades, grade 1: consistent with MRA and provide additional visual information; grade 2: consistent with MRA; grade 3: not consistent with MRA; grade 4: not consistent with MRA and provide probable misleading information). If a 3D printing vessel segment was ideally matched to the MRA findings (grade 2 or 1), a successful 3D printing was defined. Seven responders marked "grade 1" to 3D printing results, while one marked "grade 4". Therefore, 87.5% of the clinicians considered the 3D printing were successful. Our pilot study confirms the feasibility of using 3D printing technique in the research field of intracranial artery diseases. Further investigations are warranted to optimize this technique and translate it into clinical practice.

  10. D3D augmented reality imaging system: proof of concept in mammography

    Directory of Open Access Journals (Sweden)

    Douglas DB

    2016-08-01

    Full Text Available David B Douglas,1 Emanuel F Petricoin,2 Lance Liotta,2 Eugene Wilson3 1Department of Radiology, Stanford University, Palo Alto, CA, 2Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, 3Department of Radiology, Fort Benning, Columbus, GA, USA Purpose: The purpose of this article is to present images from simulated breast microcalcifications and assess the pattern of the microcalcifications with a technical development called “depth 3-dimensional (D3D augmented reality”. Materials and methods: A computer, head display unit, joystick, D3D augmented reality software, and an in-house script of simulated data of breast microcalcifications in a ductal distribution were used. No patient data was used and no statistical analysis was performed. Results: The D3D augmented reality system demonstrated stereoscopic depth perception by presenting a unique image to each eye, focal point convergence, head position tracking, 3D cursor, and joystick fly-through. Conclusion: The D3D augmented reality imaging system offers image viewing with depth perception and focal point convergence. The D3D augmented reality system should be tested to determine its utility in clinical practice. Keywords: augmented reality, 3D medical imaging, radiology, depth perception

  11. 3D Convolutional Neural Networks for Crop Classification with Multi-Temporal Remote Sensing Images

    Directory of Open Access Journals (Sweden)

    Shunping Ji

    2018-01-01

    Full Text Available This study describes a novel three-dimensional (3D convolutional neural networks (CNN based method that automatically classifies crops from spatio-temporal remote sensing images. First, 3D kernel is designed according to the structure of multi-spectral multi-temporal remote sensing data. Secondly, the 3D CNN framework with fine-tuned parameters is designed for training 3D crop samples and learning spatio-temporal discriminative representations, with the full crop growth cycles being preserved. In addition, we introduce an active learning strategy to the CNN model to improve labelling accuracy up to a required threshold with the most efficiency. Finally, experiments are carried out to test the advantage of the 3D CNN, in comparison to the two-dimensional (2D CNN and other conventional methods. Our experiments show that the 3D CNN is especially suitable in characterizing the dynamics of crop growth and outperformed the other mainstream methods.

  12. Hands-on guide for 3D image creation for geological purposes

    Science.gov (United States)

    Frehner, Marcel; Tisato, Nicola

    2013-04-01

    Geological structures in outcrops or hand specimens are inherently three dimensional (3D), and therefore better understandable if viewed in 3D. While 3D models can easily be created, manipulated, and looked at from all sides on the computer screen (e.g., using photogrammetry or laser scanning data), 3D visualizations for publications or conference posters are much more challenging as they have to live in a 2D-world (i.e., on a sheet of paper). Perspective 2D visualizations of 3D models do not fully transmit the "feeling and depth of the third dimension" to the audience; but this feeling is desirable for a better examination and understanding in 3D of the structure under consideration. One of the very few possibilities to generate real 3D images, which work on a 2D display, is by using so-called stereoscopic images. Stereoscopic images are two images of the same object recorded from two slightly offset viewpoints. Special glasses and techniques have to be used to make sure that one image is seen only by one eye, and the other image is seen by the other eye, which together lead to the "3D effect". Geoscientists are often familiar with such 3D images. For example, geomorphologists traditionally view stereographic orthophotos by employing a mirror-steroscope. Nowadays, petroleum-geoscientists examine high-resolution 3D seismic data sets in special 3D visualization rooms. One of the methods for generating and viewing a stereoscopic image, which does not require a high-tech viewing device, is to create a so-called anaglyph. The principle is to overlay two images saturated in red and cyan, respectively. The two images are then viewed through red-cyan-stereoscopic glasses. This method is simple and cost-effective, but has some drawbacks in preserving colors accurately. A similar method is used in 3D movies, where polarized light or shuttering techniques are used to separate the left from the right image, which allows preserving the original colors. The advantage of red

  13. 3-D Image Encryption Based on Rubik's Cube and RC6 Algorithm

    Science.gov (United States)

    Helmy, Mai; El-Rabaie, El-Sayed M.; Eldokany, Ibrahim M.; El-Samie, Fathi E. Abd

    2017-12-01

    A novel encryption algorithm based on the 3-D Rubik's cube is proposed in this paper to achieve 3D encryption of a group of images. This proposed encryption algorithm begins with RC6 as a first step for encrypting multiple images, separately. After that, the obtained encrypted images are further encrypted with the 3-D Rubik's cube. The RC6 encrypted images are used as the faces of the Rubik's cube. From the concepts of image encryption, the RC6 algorithm adds a degree of diffusion, while the Rubik's cube algorithm adds a degree of permutation. The simulation results demonstrate that the proposed encryption algorithm is efficient, and it exhibits strong robustness and security. The encrypted images are further transmitted over wireless Orthogonal Frequency Division Multiplexing (OFDM) system and decrypted at the receiver side. Evaluation of the quality of the decrypted images at the receiver side reveals good results.

  14. 3D Fast Spin Echo T2-weighted Contrast for Imaging the Female Cervix

    Science.gov (United States)

    Vargas Sanchez, Andrea Fernanda

    Magnetic Resonance Imaging (MRI) with T2-weighted contrast is the preferred modality for treatment planning and monitoring of cervical cancer. Current clinical protocols image the volume of interest multiple times with two dimensional (2D) T2-weighted MRI techniques. It is of interest to replace these multiple 2D acquisitions with a single three dimensional (3D) MRI acquisition to save time. However, at present the image contrast of standard 3D MRI does not distinguish cervical healthy tissue from cancerous tissue. The purpose of this thesis is to better understand the underlying factors that govern the contrast of 3D MRI and exploit this understanding via sequence modifications to improve the contrast. Numerical simulations are developed to predict observed contrast alterations and to propose an improvement. Improvements of image contrast are shown in simulation and with healthy volunteers. Reported results are only preliminary but a promising start to establish definitively 3D MRI for cervical cancer applications.

  15. Extracting 3D parametric curves from 2D images of helical objects.

    OpenAIRE

    Willcocks, Chris; Jackson, Philip T.G.; Nelson, Carl J.; Obara, Boguslaw

    2016-01-01

    Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and...

  16. Wide area 2D/3D imaging development, analysis and applications

    CERN Document Server

    Langmann, Benjamin

    2014-01-01

    Imaging technology is an important research area and it is widely utilized in a growing number of disciplines ranging from gaming, robotics and automation to medicine. In the last decade 3D imaging became popular mainly driven by the introduction of novel 3D cameras and measuring devices. These cameras are usually limited to indoor scenes with relatively low distances. Benjamin Langmann introduces medium and long-range 2D/3D cameras to overcome these limitations. He reports measurement results for these devices and studies their characteristic behavior. In order to facilitate the application o

  17. The Multiscale Bowler-Hat Transform for Vessel Enhancement in 3D Biomedical Images

    OpenAIRE

    Sazak, Cigdem; Nelson, Carl J.; Obara, Boguslaw

    2018-01-01

    Enhancement and detection of 3D vessel-like structures has long been an open problem as most existing image processing methods fail in many aspects, including a lack of uniform enhancement between vessels of different radii and a lack of enhancement at the junctions. Here, we propose a method based on mathematical morphology to enhance 3D vessel-like structures in biomedical images. The proposed method, 3D bowler-hat transform, combines sphere and line structuring elements to enhance vessel-l...

  18. A novel modeling method for manufacturing hearing aid using 3D medical images

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyeong Gyun [Dept of Radiological Science, Far East University, Eumseong (Korea, Republic of)

    2016-06-15

    This study aimed to suggest a novel method of modeling a hearing aid ear shell based on Digital Imaging and Communication in Medicine (DICOM) in the hearing aid ear shell manufacturing method using a 3D printer. In the experiment, a 3D external auditory meatus was extracted by using the critical values in the DICOM volume images, a nd t he modeling surface structures were compared in standard type STL (STereoLithography) files which could be recognized by a 3D printer. In this 3D modeling method, a conventional ear model was prepared, and the gaps between adjacent isograms produced by a 3D scanner were filled with 3D surface fragments to express the modeling structure. In this study, the same type of triangular surface structures were prepared by using the DICOM images. The result showed that the modeling surface structure based on the DICOM images provide the same environment that the conventional 3D printers may recognize, eventually enabling to print out the hearing aid ear shell shape.

  19. A novel modeling method for manufacturing hearing aid using 3D medical images

    International Nuclear Information System (INIS)

    Kim, Hyeong Gyun

    2016-01-01

    This study aimed to suggest a novel method of modeling a hearing aid ear shell based on Digital Imaging and Communication in Medicine (DICOM) in the hearing aid ear shell manufacturing method using a 3D printer. In the experiment, a 3D external auditory meatus was extracted by using the critical values in the DICOM volume images, a nd t he modeling surface structures were compared in standard type STL (STereoLithography) files which could be recognized by a 3D printer. In this 3D modeling method, a conventional ear model was prepared, and the gaps between adjacent isograms produced by a 3D scanner were filled with 3D surface fragments to express the modeling structure. In this study, the same type of triangular surface structures were prepared by using the DICOM images. The result showed that the modeling surface structure based on the DICOM images provide the same environment that the conventional 3D printers may recognize, eventually enabling to print out the hearing aid ear shell shape

  20. Signal alteration of the cochlear perilymph on 3 different sequences after intratympanic Gd-DTPA administration at 3 tesla. Comparison of 3D-FLAIR, 3D-T1-weighted imaging, and 3D-CISS

    International Nuclear Information System (INIS)

    Yamazaki, Masahiro; Naganawa, Shinji; Kawai, Hisashi; Nihashi, Takashi; Nakashima, Tsutomu

    2010-01-01

    Three-dimensional fluid-attenuated inversion recovery (3D-FLAIR) imaging after intratympanic gadolinium injection is useful for pathophysiologic and morphologic analysis of the inner ear. However, statistical analysis of differences in inner ear signal intensity among 3D-FLAIR and other sequences has not been reported. We evaluated the signal intensity of cochlear fluid on each of 3D-FLAIR, 3D-T 1 -weighted imaging (T 1 WI), and 3D-constructive interference in the steady state (CISS) to clarify the differences in contrast effect among these 3 sequences using intratympanic gadolinium injection. Twenty-one patients underwent 3D-FLAIR, 3D-T 1 WI, and 3D-CISS imaging at 3 tesla 24 hours after intratympanic injection of gadolinium. We determined regions of interest of the cochleae (C) and medulla oblongata (M) on each image, evaluated the signal intensity ratio between C and M (CM ratio), and determined the ratio of cochlear signal intensity of the injected side to that of the non-injected side (contrast value). The CM ratio of the injected side (3.00±1.31, range, 0.53 to 4.88, on 3D-FLAIR; 0.83±0.30, range, 0.36 to 1.58 on 3D-T 1 WI) was significantly higher than that of the non-injected side (0.52±0.14, range, 0.30 to 0.76 on 3D-FLAIR; 0.49±0.11, range, 0.30 to 0.71 on 3D-T 1 WI) on 3D-FLAIR and 3D-T 1 WI (P 1 WI (1.73±0.60 range, 0.98 to 3.09) (P<0.001). The 3D-FLAIR sequence is the most sensitive for observing alteration in inner ear fluid signal after intratympanic gadolinium injection. Our results warrant use of 3D-FLAIR as a sensitive imaging technique to clarify the pathological and morphological mechanisms of disorders of the inner ear. (author)

  1. Integration of virtual and real scenes within an integral 3D imaging environment

    Science.gov (United States)

    Ren, Jinsong; Aggoun, Amar; McCormick, Malcolm

    2002-11-01

    The Imaging Technologies group at De Montfort University has developed an integral 3D imaging system, which is seen as the most likely vehicle for 3D television avoiding psychological effects. To create real fascinating three-dimensional television programs, a virtual studio that performs the task of generating, editing and integrating the 3D contents involving virtual and real scenes is required. The paper presents, for the first time, the procedures, factors and methods of integrating computer-generated virtual scenes with real objects captured using the 3D integral imaging camera system. The method of computer generation of 3D integral images, where the lens array is modelled instead of the physical camera is described. In the model each micro-lens that captures different elemental images of the virtual scene is treated as an extended pinhole camera. An integration process named integrated rendering is illustrated. Detailed discussion and deep investigation are focused on depth extraction from captured integral 3D images. The depth calculation method from the disparity and the multiple baseline method that is used to improve the precision of depth estimation are also presented. The concept of colour SSD and its further improvement in the precision is proposed and verified.

  2. Rapid 3D Reconstruction for Image Sequence Acquired from UAV Camera

    Directory of Open Access Journals (Sweden)

    Yufu Qu

    2018-01-01

    Full Text Available In order to reconstruct three-dimensional (3D structures from an image sequence captured by unmanned aerial vehicles’ camera (UAVs and improve the processing speed, we propose a rapid 3D reconstruction method that is based on an image queue, considering the continuity and relevance of UAV camera images. The proposed approach first compresses the feature points of each image into three principal component points by using the principal component analysis method. In order to select the key images suitable for 3D reconstruction, the principal component points are used to estimate the interrelationships between images. Second, these key images are inserted into a fixed-length image queue. The positions and orientations of the images are calculated, and the 3D coordinates of the feature points are estimated using weighted bundle adjustment. With this structural information, the depth maps of these images can be calculated. Next, we update the image queue by deleting some of the old images and inserting some new images into the queue, and a structural calculation of all the images can be performed by repeating the previous steps. Finally, a dense 3D point cloud can be obtained using the depth–map fusion method. The experimental results indicate that when the texture of the images is complex and the number of images exceeds 100, the proposed method can improve the calculation speed by more than a factor of four with almost no loss of precision. Furthermore, as the number of images increases, the improvement in the calculation speed will become more noticeable.

  3. Rapid 3D Reconstruction for Image Sequence Acquired from UAV Camera.

    Science.gov (United States)

    Qu, Yufu; Huang, Jianyu; Zhang, Xuan

    2018-01-14

    In order to reconstruct three-dimensional (3D) structures from an image sequence captured by unmanned aerial vehicles' camera (UAVs) and improve the processing speed, we propose a rapid 3D reconstruction method that is based on an image queue, considering the continuity and relevance of UAV camera images. The proposed approach first compresses the feature points of each image into three principal component points by using the principal component analysis method. In order to select the key images suitable for 3D reconstruction, the principal component points are used to estimate the interrelationships between images. Second, these key images are inserted into a fixed-length image queue. The positions and orientations of the images are calculated, and the 3D coordinates of the feature points are estimated using weighted bundle adjustment. With this structural information, the depth maps of these images can be calculated. Next, we update the image queue by deleting some of the old images and inserting some new images into the queue, and a structural calculation of all the images can be performed by repeating the previous steps. Finally, a dense 3D point cloud can be obtained using the depth-map fusion method. The experimental results indicate that when the texture of the images is complex and the number of images exceeds 100, the proposed method can improve the calculation speed by more than a factor of four with almost no loss of precision. Furthermore, as the number of images increases, the improvement in the calculation speed will become more noticeable.

  4. Performance evaluation of 3-D enhancement filters for detection of lung cancer from 3-D chest X-ray CT images

    International Nuclear Information System (INIS)

    Shimizu, Akinobu; Hagai, Makoto; Toriwaki, Jun-ichiro; Hasegawa, Jun-ichi.

    1995-01-01

    This paper evaluates the performance of several three dimensional enhancement filters used in procedures for detecting lung cancer shadows from three dimensional (3D) chest X-ray CT images. Two dimensional enhancement filters such as Min-DD filter, Contrast filter and N-Quoit filter have been proposed for enhancing cancer shadows in conventional 2D X-ray images. In this paper, we extend each of these 2D filters to a 3D filter and evaluate its performance experimentally by using CT images with artificial and true lung cancer shadows. As a result, we find that these 3D filters are effective for determining the position of a lung cancer shadow in a 3D chest CT image, as compared with the simple procedure such as smoothing filter, and that the performance of these filters become lower in the hilar area due to the influence of the vessel shadows. (author)

  5. A multiphoton laser scanning microscope setup for transcranial in vivo brain imaging on mice

    Science.gov (United States)

    Nase, Gabriele; Helm, P. Johannes; Reppen, Trond; Ottersen, Ole Petter

    2005-12-01

    We describe a multiphoton laser scanning microscope setup for transcranial in vivo brain imaging in mice. The modular system is based on a modified industrial standard Confocal Scanning Laser Microscope (CSLM) and is assembled mainly from commercially available components. A special multifunctional stage, which is optimized for both laser scanning microscopic observation and preparative animal surgery, has been developed and built. The detection unit includes a highly efficient photomultiplier tube installed in a Peltier-cooled thermal box shielding the detector from changes in room temperature and from distortions caused by external electromagnetic fields. The images are recorded using a 12-bit analog-to-digital converter. Depending on the characteristics of the staining, individual nerve cells can be imaged down to at least 100μm below the intact cranium and down to at least 200μm below the opened cranium.

  6. Artificial intelligence (AI)-based relational matching and multimodal medical image fusion: generalized 3D approaches

    Science.gov (United States)

    Vajdic, Stevan M.; Katz, Henry E.; Downing, Andrew R.; Brooks, Michael J.

    1994-09-01

    A 3D relational image matching/fusion algorithm is introduced. It is implemented in the domain of medical imaging and is based on Artificial Intelligence paradigms--in particular, knowledge base representation and tree search. The 2D reference and target images are selected from 3D sets and segmented into non-touching and non-overlapping regions, using iterative thresholding and/or knowledge about the anatomical shapes of human organs. Selected image region attributes are calculated. Region matches are obtained using a tree search, and the error is minimized by evaluating a `goodness' of matching function based on similarities of region attributes. Once the matched regions are found and the spline geometric transform is applied to regional centers of gravity, images are ready for fusion and visualization into a single 3D image of higher clarity.

  7. The Application of the Technology of 3D Satellite Cloud Imaging in Virtual Reality Simulation

    Directory of Open Access Journals (Sweden)

    Xiao-fang Xie

    2007-05-01

    Full Text Available Using satellite cloud images to simulate clouds is one of the new visual simulation technologies in Virtual Reality (VR. Taking the original data of satellite cloud images as the source, this paper depicts specifically the technology of 3D satellite cloud imaging through the transforming of coordinates and projection, creating a DEM (Digital Elevation Model of cloud imaging and 3D simulation. A Mercator projection was introduced to create a cloud image DEM, while solutions for geodetic problems were introduced to calculate distances, and the outer-trajectory science of rockets was introduced to obtain the elevation of clouds. For demonstration, we report on a computer program to simulate the 3D satellite cloud images.

  8. Coupling 2D/3D registration method and statistical model to perform 3D reconstruction from partial x-rays images data.

    Science.gov (United States)

    Cresson, T; Chav, R; Branchaud, D; Humbert, L; Godbout, B; Aubert, B; Skalli, W; De Guise, J A

    2009-01-01

    3D reconstructions of the spine from a frontal and sagittal radiographs is extremely challenging. The overlying features of soft tissues and air cavities interfere with image processing. It is also difficult to obtain information that is accurate enough to reconstruct complete 3D models. To overcome these problems, the proposed method efficiently combines the partial information contained in two images from a patient with a statistical 3D spine model generated from a database of scoliotic patients. The algorithm operates through two simultaneous iterating processes. The first one generates a personalized vertebra model using a 2D/3D registration process with bone boundaries extracted from radiographs, while the other one infers the position and the shape of other vertebrae from the current estimation of the registration process using a statistical 3D model. Experimental evaluations have shown good performances of the proposed approach in terms of accuracy and robustness when compared to CT-scan.

  9. Applications of Panoramic Images: from 720° Panorama to Interior 3d Models of Augmented Reality

    Science.gov (United States)

    Lee, I.-C.; Tsai, F.

    2015-05-01

    A series of panoramic images are usually used to generate a 720° panorama image. Although panoramic images are typically used for establishing tour guiding systems, in this research, we demonstrate the potential of using panoramic images acquired from multiple sites to create not only 720° panorama, but also three-dimensional (3D) point clouds and 3D indoor models. Since 3D modeling is one of the goals of this research, the location of the panoramic sites needed to be carefully planned in order to maintain a robust result for close-range photogrammetry. After the images are acquired, panoramic images are processed into 720° panoramas, and these panoramas which can be used directly as panorama guiding systems or other applications. In addition to these straightforward applications, interior orientation parameters can also be estimated while generating 720° panorama. These parameters are focal length, principle point, and lens radial distortion. The panoramic images can then be processed with closerange photogrammetry procedures to extract the exterior orientation parameters and generate 3D point clouds. In this research, VisaulSFM, a structure from motion software is used to estimate the exterior orientation, and CMVS toolkit is used to generate 3D point clouds. Next, the 3D point clouds are used as references to create building interior models. In this research, Trimble Sketchup was used to build the model, and the 3D point cloud was added to the determining of locations of building objects using plane finding procedure. In the texturing process, the panorama images are used as the data source for creating model textures. This 3D indoor model was used as an Augmented Reality model replacing a guide map or a floor plan commonly used in an on-line touring guide system. The 3D indoor model generating procedure has been utilized in two research projects: a cultural heritage site at Kinmen, and Taipei Main Station pedestrian zone guidance and navigation system. The

  10. 3D shape recovery from image focus using gray level co-occurrence matrix

    Science.gov (United States)

    Mahmood, Fahad; Munir, Umair; Mehmood, Fahad; Iqbal, Javaid

    2018-04-01

    Recovering a precise and accurate 3-D shape of the target object utilizing robust 3-D shape recovery algorithm is an ultimate objective of computer vision community. Focus measure algorithm plays an important role in this architecture which convert the color values of each pixel of the acquired 2-D image dataset into corresponding focus values. After convolving the focus measure filter with the input 2-D image dataset, a 3-D shape recovery approach is applied which will recover the depth map. In this document, we are concerned with proposing Gray Level Co-occurrence Matrix along with its statistical features for computing the focus information of the image dataset. The Gray Level Co-occurrence Matrix quantifies the texture present in the image using statistical features and then applies joint probability distributive function of the gray level pairs of the input image. Finally, we quantify the focus value of the input image using Gaussian Mixture Model. Due to its little computational complexity, sharp focus measure curve, robust to random noise sources and accuracy, it is considered as superior alternative to most of recently proposed 3-D shape recovery approaches. This algorithm is deeply investigated on real image sequences and synthetic image dataset. The efficiency of the proposed scheme is also compared with the state of art 3-D shape recovery approaches. Finally, by means of two global statistical measures, root mean square error and correlation, we claim that this approach -in spite of simplicity generates accurate results.

  11. 2D-Driven 3D Object Detection in RGB-D Images

    KAUST Repository

    Lahoud, Jean

    2017-12-25

    In this paper, we present a technique that places 3D bounding boxes around objects in an RGB-D scene. Our approach makes best use of the 2D information to quickly reduce the search space in 3D, benefiting from state-of-the-art 2D object detection techniques. We then use the 3D information to orient, place, and score bounding boxes around objects. We independently estimate the orientation for every object, using previous techniques that utilize normal information. Object locations and sizes in 3D are learned using a multilayer perceptron (MLP). In the final step, we refine our detections based on object class relations within a scene. When compared to state-of-the-art detection methods that operate almost entirely in the sparse 3D domain, extensive experiments on the well-known SUN RGB-D dataset [29] show that our proposed method is much faster (4.1s per image) in detecting 3D objects in RGB-D images and performs better (3 mAP higher) than the state-of-the-art method that is 4.7 times slower and comparably to the method that is two orders of magnitude slower. This work hints at the idea that 2D-driven object detection in 3D should be further explored, especially in cases where the 3D input is sparse.

  12. From 2D PET to 3D PET. Issues of data representation and image reconstruction

    International Nuclear Information System (INIS)

    Gundlich, B.; Musmann, P.; Weber, S.; Nix, O.; Semmler, W.

    2006-01-01

    Positron emission tomography (PET), intrinsically a 3D imaging technique, was for a long time exclusively operated in 2D mode, using septa to shield the detectors from photons emitted obliquely to the detector planes. However, the use of septa results in a considerable loss of sensitivity. From the late 1980s, significant efforts have been made to develop a methodology for the acquisition and reconstruction of 3D PET data. This paper focuses on the differences between data acquisition in 2D and 3D mode, especially in terms of data set sizes and representation. Although the real time data acquisition aspect in 3D has been mostly solved in modern PET scanner systems, there still remain questions on how to represent and how to make best use of the information contained in the acquired data sets. Data representation methods, such as list-mode and matrix-based methods, possibly with additional compression, will be discussed. Moving from 2D to 3D PET has major implications on the way these data are reconstructed to images. Two fundamentally different approaches exist, the analytical one and the iterative one. Both, at different expenses, can be extended to directly handle 3D data sets. Either way the computational burden increases heavily compared to 2D reconstruction. One possibility to benefit from the increased sensitivity in 3D PET while sticking to high-performance 2D reconstruction algorithms is to rebin 3D into 2D data sets. The value of data rebinning will be explored. An ever increasing computing power and the concept of distributed or parallel computing have made direct 3D reconstruction feasible. Following a short review of reconstruction methods and their extensions to 3D, we focus on numerical aspects that improve reconstruction performance, which is especially important in solving large equation systems in 3D iterative reconstruction. Finally exemplary results are shown to review the properties of the discussed algorithms. (orig.)

  13. 3D reconstruction of SEM images by use of optical photogrammetry software.

    Science.gov (United States)

    Eulitz, Mona; Reiss, Gebhard

    2015-08-01

    Reconstruction of the three-dimensional (3D) surface of an object to be examined is widely used for structure analysis in science and many biological questions require information about their true 3D structure. For Scanning Electron Microscopy (SEM) there has been no efficient non-destructive solution for reconstruction of the surface morphology to date. The well-known method of recording stereo pair images generates a 3D stereoscope reconstruction of a section, but not of the complete sample surface. We present a simple and non-destructive method of 3D surface reconstruction from SEM samples based on the principles of optical close range photogrammetry. In optical close range photogrammetry a series of overlapping photos is used to generate a 3D model of the surface of an object. We adapted this method to the special SEM requirements. Instead of moving a detector around the object, the object itself was rotated. A series of overlapping photos was stitched and converted into a 3D model using the software commonly used for optical photogrammetry. A rabbit kidney glomerulus was used to demonstrate the workflow of this adaption. The reconstruction produced a realistic and high-resolution 3D mesh model of the glomerular surface. The study showed that SEM micrographs are suitable for 3D reconstruction by optical photogrammetry. This new approach is a simple and useful method of 3D surface reconstruction and suitable for various applications in research and teaching. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Mixed reality orthognathic surgical simulation by entity model manipulation and 3D-image display

    Science.gov (United States)

    Shimonagayoshi, Tatsunari; Aoki, Yoshimitsu; Fushima, Kenji; Kobayashi, Masaru

    2005-12-01

    In orthognathic surgery, the framing of 3D-surgical planning that considers the balance between the front and back positions and the symmetry of the jawbone, as well as the dental occlusion of teeth, is essential. In this study, a support system for orthodontic surgery to visualize the changes in the mandible and the occlusal condition and to determine the optimum position in mandibular osteotomy has been developed. By integrating the operating portion of a tooth model that is to determine the optimum occlusal position by manipulating the entity tooth model and the 3D-CT skeletal images (3D image display portion) that are simultaneously displayed in real-time, the determination of the mandibular position and posture in which the improvement of skeletal morphology and occlusal condition is considered, is possible. The realistic operation of the entity model and the virtual 3D image display enabled the construction of a surgical simulation system that involves augmented reality.

  15. Augmented Reality Imaging System: 3D Viewing of a Breast Cancer.

    Science.gov (United States)

    Douglas, David B; Boone, John M; Petricoin, Emanuel; Liotta, Lance; Wilson, Eugene

    2016-01-01

    To display images of breast cancer from a dedicated breast CT using Depth 3-Dimensional (D3D) augmented reality. A case of breast cancer imaged using contrast-enhanced breast CT (Computed Tomography) was viewed with the augmented reality imaging, which uses a head display unit (HDU) and joystick control interface. The augmented reality system demonstrated 3D viewing of the breast mass with head position tracking, stereoscopic depth perception, focal point convergence and the use of a 3D cursor and joy-stick enabled fly through with visualization of the spiculations extending from the breast cancer. The augmented reality system provided 3D visualization of the breast cancer with depth perception and visualization of the mass's spiculations. The augmented reality system should be further researched to determine the utility in clinical practice.

  16. 3D digital stereophotogrammetry: a practical guide to facial image acquisition

    Directory of Open Access Journals (Sweden)

    Upson Kristen

    2010-07-01

    Full Text Available Abstract The use of 3D surface imaging technology is becoming increasingly common in craniofacial clinics and research centers. Due to fast capture speeds and ease of use, 3D digital stereophotogrammetry is quickly becoming the preferred facial surface imaging modality. These systems can serve as an unparalleled tool for craniofacial surgeons, proving an objective digital archive of the patient's face without exposure to radiation. Acquiring consistent high-quality 3D facial captures requires planning and knowledge of the limitations of these devices. Currently, there are few resources available to help new users of this technology with the challenges they will inevitably confront. To address this deficit, this report will highlight a number of common issues that can interfere with the 3D capture process and offer practical solutions to optimize image quality.

  17. Technical Note: A 3-D rendering algorithm for electromechanical wave imaging of a beating heart.

    Science.gov (United States)

    Nauleau, Pierre; Melki, Lea; Wan, Elaine; Konofagou, Elisa

    2017-09-01

    Arrhythmias can be treated by ablating the heart tissue in the regions of abnormal contraction. The current clinical standard provides electroanatomic 3-D maps to visualize the electrical activation and locate the arrhythmogenic sources. However, the procedure is time-consuming and invasive. Electromechanical wave imaging is an ultrasound-based noninvasive technique that can provide 2-D maps of the electromechanical activation of the heart. In order to fully visualize the complex 3-D pattern of activation, several 2-D views are acquired and processed separately. They are then manually registered with a 3-D rendering software to generate a pseudo-3-D map. However, this last step is operator-dependent and time-consuming. This paper presents a method to generate a full 3-D map of the electromechanical activation using multiple 2-D images. Two canine models were considered to illustrate the method: one in normal sinus rhythm and one paced from the lateral region of the heart. Four standard echographic views of each canine heart were acquired. Electromechanical wave imaging was applied to generate four 2-D activation maps of the left ventricle. The radial positions and activation timings of the walls were automatically extracted from those maps. In each slice, from apex to base, these values were interpolated around the circumference to generate a full 3-D map. In both cases, a 3-D activation map and a cine-loop of the propagation of the electromechanical wave were automatically generated. The 3-D map showing the electromechanical activation timings overlaid on realistic anatomy assists with the visualization of the sources of earlier activation (which are potential arrhythmogenic sources). The earliest sources of activation corresponded to the expected ones: septum for the normal rhythm and lateral for the pacing case. The proposed technique provides, automatically, a 3-D electromechanical activation map with a realistic anatomy. This represents a step towards a

  18. Feasibility of fabricating personalized 3D-printed bone grafts guided by high-resolution imaging

    Science.gov (United States)

    Hong, Abigail L.; Newman, Benjamin T.; Khalid, Arbab; Teter, Olivia M.; Kobe, Elizabeth A.; Shukurova, Malika; Shinde, Rohit; Sipzner, Daniel; Pignolo, Robert J.; Udupa, Jayaram K.; Rajapakse, Chamith S.

    2017-03-01

    Current methods of bone graft treatment for critical size bone defects can give way to several clinical complications such as limited available bone for autografts, non-matching bone structure, lack of strength which can compromise a patient's skeletal system, and sterilization processes that can prevent osteogenesis in the case of allografts. We intend to overcome these disadvantages by generating a patient-specific 3D printed bone graft guided by high-resolution medical imaging. Our synthetic model allows us to customize the graft for the patients' macro- and microstructure and correct any structural deficiencies in the re-meshing process. These 3D-printed models can presumptively serve as the scaffolding for human mesenchymal stem cell (hMSC) engraftment in order to facilitate bone growth. We performed highresolution CT imaging of a cadaveric human proximal femur at 0.030-mm isotropic voxels. We used these images to generate a 3D computer model that mimics bone geometry from micro to macro scale represented by STereoLithography (STL) format. These models were then reformatted to a format that can be interpreted by the 3D printer. To assess how much of the microstructure was replicated, 3D-printed models were re-imaged using micro-CT at 0.025-mm isotropic voxels and compared to original high-resolution CT images used to generate the 3D model in 32 sub-regions. We found a strong correlation between 3D-printed bone volume and volume of bone in the original images used for 3D printing (R2 = 0.97). We expect to further refine our approach with additional testing to create a viable synthetic bone graft with clinical functionality.

  19. 3D and 4D magnetic susceptibility tomography based on complex MR images

    Science.gov (United States)

    Chen, Zikuan; Calhoun, Vince D

    2014-11-11

    Magnetic susceptibility is the physical property for T2*-weighted magnetic resonance imaging (T2*MRI). The invention relates to methods for reconstructing an internal distribution (3D map) of magnetic susceptibility values, .chi. (x,y,z), of an object, from 3D T2*MRI phase images, by using Computed Inverse Magnetic Resonance Imaging (CIMRI) tomography. The CIMRI technique solves the inverse problem of the 3D convolution by executing a 3D Total Variation (TV) regularized iterative convolution scheme, using a split Bregman iteration algorithm. The reconstruction of .chi. (x,y,z) can be designed for low-pass, band-pass, and high-pass features by using a convolution kernel that is modified from the standard dipole kernel. Multiple reconstructions can be implemented in parallel, and averaging the reconstructions can suppress noise. 4D dynamic magnetic susceptibility tomography can be implemented by reconstructing a 3D susceptibility volume from a 3D phase volume by performing 3D CIMRI magnetic susceptibility tomography at each snapshot time.

  20. 3D fluoroscopic image estimation using patient-specific 4DCBCT-based motion models

    International Nuclear Information System (INIS)

    Dhou, S; Hurwitz, M; Cai, W; Rottmann, J; Williams, C; Wagar, M; Berbeco, R; Lewis, J H; Mishra, P; Li, R; Ionascu, D

    2015-01-01

    3D fluoroscopic images represent volumetric patient anatomy during treatment with high spatial and temporal resolution. 3D fluoroscopic images estimated using motion models built using 4DCT images, taken days or weeks prior to treatment, do not reliably represent patient anatomy during treatment. In this study we developed and performed initial evaluation of techniques to develop patient-specific motion models from 4D cone-beam CT (4DCBCT) images, taken immediately before treatment, and used these models to estimate 3D fluoroscopic images based on 2D kV projections captured during treatment. We evaluate the accuracy of 3D fluoroscopic images by comparison to ground truth digital and physical phantom images. The performance of 4DCBCT-based and 4DCT-based motion models are compared in simulated clinical situations representing tumor baseline shift or initial patient positioning errors. The results of this study demonstrate the ability for 4DCBCT imaging to generate motion models that can account for changes that cannot be accounted for with 4DCT-based motion models. When simulating tumor baseline shift and patient positioning errors of up to 5 mm, the average tumor localization error and the 95th percentile error in six datasets were 1.20 and 2.2 mm, respectively, for 4DCBCT-based motion models. 4DCT-based motion models applied to the same six datasets resulted in average tumor localization error and the 95th percentile error of 4.18 and 5.4 mm, respectively. Analysis of voxel-wise intensity differences was also conducted for all experiments. In summary, this study demonstrates the feasibility of 4DCBCT-based 3D fluoroscopic image generation in digital and physical phantoms and shows the potential advantage of 4DCBCT-based 3D fluoroscopic image estimation when there are changes in anatomy between the time of 4DCT imaging and the time of treatment delivery. (paper)

  1. 3D temporal subtraction on multislice CT images using nonlinear warping technique

    Science.gov (United States)

    Ishida, Takayuki; Katsuragawa, Shigehiko; Kawashita, Ikuo; Kim, Hyounseop; Itai, Yoshinori; Awai, Kazuo; Li, Qiang; Doi, Kunio

    2007-03-01

    The detection of very subtle lesions and/or lesions overlapped with vessels on CT images is a time consuming and difficult task for radiologists. In this study, we have developed a 3D temporal subtraction method to enhance interval changes between previous and current multislice CT images based on a nonlinear image warping technique. Our method provides a subtraction CT image which is obtained by subtraction of a previous CT image from a current CT image. Reduction of misregistration artifacts is important in the temporal subtraction method. Therefore, our computerized method includes global and local image matching techniques for accurate registration of current and previous CT images. For global image matching, we selected the corresponding previous section image for each current section image by using 2D cross-correlation between a blurred low-resolution current CT image and a blurred previous CT image. For local image matching, we applied the 3D template matching technique with translation and rotation of volumes of interests (VOIs) which were selected in the current and the previous CT images. The local shift vector for each VOI pair was determined when the cross-correlation value became the maximum in the 3D template matching. The local shift vectors at all voxels were determined by interpolation of shift vectors of VOIs, and then the previous CT image was nonlinearly warped according to the shift vector for each voxel. Finally, the warped previous CT image was subtracted from the current CT image. The 3D temporal subtraction method was applied to 19 clinical cases. The normal background structures such as vessels, ribs, and heart were removed without large misregistration artifacts. Thus, interval changes due to lung diseases were clearly enhanced as white shadows on subtraction CT images.

  2. Model-Based Referenceless Quality Metric of 3D Synthesized Images Using Local Image Description.

    Science.gov (United States)

    Gu, Ke; Jakhetiya, Vinit; Qiao, Jun-Fei; Li, Xiaoli; Lin, Weisi; Thalmann, Daniel

    2017-07-28

    New challenges have been brought out along with the emerging of 3D-related technologies such as virtual reality (VR), augmented reality (AR), and mixed reality (MR). Free viewpoint video (FVV), due to its applications in remote surveillance, remote education, etc, based on the flexible selection of direction and viewpoint, has been perceived as the development direction of next-generation video technologies and has drawn a wide range of researchers' attention. Since FVV images are synthesized via a depth image-based rendering (DIBR) procedure in the "blind" environment (without reference images), a reliable real-time blind quality evaluation and monitoring system is urgently required. But existing assessment metrics do not render human judgments faithfully mainly because geometric distortions are generated by DIBR. To this end, this paper proposes a novel referenceless quality metric of DIBR-synthesized images using the autoregression (AR)-based local image description. It was found that, after the AR prediction, the reconstructed error between a DIBR-synthesized image and its AR-predicted image can accurately capture the geometry distortion. The visual saliency is then leveraged to modify the proposed blind quality metric to a sizable margin. Experiments validate the superiority of our no-reference quality method as compared with prevailing full-, reduced- and no-reference models.

  3. In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent

    Energy Technology Data Exchange (ETDEWEB)

    Matsuyama, Shigeo; Hamada, Naoki; Ishii, Keizo; Nozawa, Yuichiro; Ohkura, Satoru; Terakawa, Atsuki; Hatori, Yoshinobu; Fujiki, Kota; Fujiwara, Mitsuhiro; Toyama, Sho

    2015-04-01

    In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.

  4. Kalisphera: an analytical tool to reproduce the partial volume effect of spheres imaged in 3D

    International Nuclear Information System (INIS)

    Tengattini, Alessandro; Andò, Edward

    2015-01-01

    In experimental mechanics, where 3D imaging is having a profound effect, spheres are commonly adopted for their simplicity and for the ease of their modeling. In this contribution we develop an analytical tool, ‘kalisphera’, to produce 3D raster images of spheres including their partial volume effect. This allows us to evaluate the metrological performance of existing image-based measurement techniques (knowing a priori the ground truth). An advanced application of ‘kalisphera’ is developed here to identify and accurately characterize spheres in real 3D x-ray tomography images with the objective of improving trinarization and contact detection. The effect of the common experimental imperfections is assessed and the overall performance of the tool tested on real images. (paper)

  5. Technical evaluation of DIC helical CT and 3D image for laparoscopic cholecystectomy

    International Nuclear Information System (INIS)

    Shibuya, Kouki; Uchimura, Fumiaki; Haga, Tomo

    1995-01-01

    Recently Laparoscopic Cholecystectomy (L.C.) was widely accepted for its low invasive procedure. Before L.C., it is important to understand anatomical recognization of biliary tree. We examined DIC Helical CT before L.C., and reconstructed 3D Cholangiographic image. We evaluated physical potentiality of Helical CT using Section Sensitivity Profiles (SSP) with 5, 10 mm slice thickness on 360deg linear interpolation. And we analyzed most useful 3D image for biliary tree. Results showed the SSP depended on slice thickness (X-ray beam width) and table movement at same reconstruction spacing. The peak of SSP depended on slice thickness (X-ray beam width) and reconstruction spacing at same table movement. Clinically, it was necessary under 5 mm/rotation table movement and 5 mm thickness for acquiring volume image data. 3D Cholangiographic image reconstructed with 1 mm spacing image was useful in evaluation of relationship of anatomical biliary tree. (author)

  6. Technical Note: Characterization of custom 3D printed multimodality imaging phantoms

    Energy Technology Data Exchange (ETDEWEB)

    Bieniosek, Matthew F. [Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, California 94305 (United States); Lee, Brian J. [Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Stanford, California 94305 (United States); Levin, Craig S., E-mail: cslevin@stanford.edu [Departments of Radiology, Physics, Bioengineering and Electrical Engineering, Stanford University, 300 Pasteur Dr., Stanford, California 94305-5128 (United States)

    2015-10-15

    Purpose: Imaging phantoms are important tools for researchers and technicians, but they can be costly and difficult to customize. Three dimensional (3D) printing is a widely available rapid prototyping technique that enables the fabrication of objects with 3D computer generated geometries. It is ideal for quickly producing customized, low cost, multimodal, reusable imaging phantoms. This work validates the use of 3D printed phantoms by comparing CT and PET scans of a 3D printed phantom and a commercial “Micro Deluxe” phantom. This report also presents results from a customized 3D printed PET/MRI phantom, and a customized high resolution imaging phantom with sub-mm features. Methods: CT and PET scans of a 3D printed phantom and a commercial Micro Deluxe (Data Spectrum Corporation, USA) phantom with 1.2, 1.6, 2.4, 3.2, 4.0, and 4.8 mm diameter hot rods were acquired. The measured PET and CT rod sizes, activities, and attenuation coefficients were compared. A PET/MRI scan of a custom 3D printed phantom with hot and cold rods was performed, with photon attenuation and normalization measurements performed with a separate 3D printed normalization phantom. X-ray transmission scans of a customized two level high resolution 3D printed phantom with sub-mm features were also performed. Results: Results show very good agreement between commercial and 3D printed micro deluxe phantoms with less than 3% difference in CT measured rod diameter, less than 5% difference in PET measured rod diameter, and a maximum of 6.2% difference in average rod activity from a 10 min, 333 kBq/ml (9 μCi/ml) Siemens Inveon (Siemens Healthcare, Germany) PET scan. In all cases, these differences were within the measurement uncertainties of our setups. PET/MRI scans successfully identified 3D printed hot and cold rods on PET and MRI modalities. X-ray projection images of a 3D printed high resolution phantom identified features as small as 350 μm wide. Conclusions: This work shows that 3D printed

  7. Technical Note: Characterization of custom 3D printed multimodality imaging phantoms

    International Nuclear Information System (INIS)

    Bieniosek, Matthew F.; Lee, Brian J.; Levin, Craig S.

    2015-01-01

    Purpose: Imaging phantoms are important tools for researchers and technicians, but they can be costly and difficult to customize. Three dimensional (3D) printing is a widely available rapid prototyping technique that enables the fabrication of objects with 3D computer generated geometries. It is ideal for quickly producing customized, low cost, multimodal, reusable imaging phantoms. This work validates the use of 3D printed phantoms by comparing CT and PET scans of a 3D printed phantom and a commercial “Micro Deluxe” phantom. This report also presents results from a customized 3D printed PET/MRI phantom, and a customized high resolution imaging phantom with sub-mm features. Methods: CT and PET scans of a 3D printed phantom and a commercial Micro Deluxe (Data Spectrum Corporation, USA) phantom with 1.2, 1.6, 2.4, 3.2, 4.0, and 4.8 mm diameter hot rods were acquired. The measured PET and CT rod sizes, activities, and attenuation coefficients were compared. A PET/MRI scan of a custom 3D printed phantom with hot and cold rods was performed, with photon attenuation and normalization measurements performed with a separate 3D printed normalization phantom. X-ray transmission scans of a customized two level high resolution 3D printed phantom with sub-mm features were also performed. Results: Results show very good agreement between commercial and 3D printed micro deluxe phantoms with less than 3% difference in CT measured rod diameter, less than 5% difference in PET measured rod diameter, and a maximum of 6.2% difference in average rod activity from a 10 min, 333 kBq/ml (9 μCi/ml) Siemens Inveon (Siemens Healthcare, Germany) PET scan. In all cases, these differences were within the measurement uncertainties of our setups. PET/MRI scans successfully identified 3D printed hot and cold rods on PET and MRI modalities. X-ray projection images of a 3D printed high resolution phantom identified features as small as 350 μm wide. Conclusions: This work shows that 3D printed

  8. Technical Note: Characterization of custom 3D printed multimodality imaging phantoms.

    Science.gov (United States)

    Bieniosek, Matthew F; Lee, Brian J; Levin, Craig S

    2015-10-01

    Imaging phantoms are important tools for researchers and technicians, but they can be costly and difficult to customize. Three dimensional (3D) printing is a widely available rapid prototyping technique that enables the fabrication of objects with 3D computer generated geometries. It is ideal for quickly producing customized, low cost, multimodal, reusable imaging phantoms. This work validates the use of 3D printed phantoms by comparing CT and PET scans of a 3D printed phantom and a commercial "Micro Deluxe" phantom. This report also presents results from a customized 3D printed PET/MRI phantom, and a customized high resolution imaging phantom with sub-mm features. CT and PET scans of a 3D printed phantom and a commercial Micro Deluxe (Data Spectrum Corporation, USA) phantom with 1.2, 1.6, 2.4, 3.2, 4.0, and 4.8 mm diameter hot rods were acquired. The measured PET and CT rod sizes, activities, and attenuation coefficients were compared. A PET/MRI scan of a custom 3D printed phantom with hot and cold rods was performed, with photon attenuation and normalization measurements performed with a separate 3D printed normalization phantom. X-ray transmission scans of a customized two level high resolution 3D printed phantom with sub-mm features were also performed. Results show very good agreement between commercial and 3D printed micro deluxe phantoms with less than 3% difference in CT measured rod diameter, less than 5% difference in PET measured rod diameter, and a maximum of 6.2% difference in average rod activity from a 10 min, 333 kBq/ml (9 μCi/ml) Siemens Inveon (Siemens Healthcare, Germany) PET scan. In all cases, these differences were within the measurement uncertainties of our setups. PET/MRI scans successfully identified 3D printed hot and cold rods on PET and MRI modalities. X-ray projection images of a 3D printed high resolution phantom identified features as small as 350 μm wide. This work shows that 3D printed phantoms can be functionally equivalent to

  9. From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin

    Science.gov (United States)

    Huck, Volker; Gorzelanny, Christian; Thomas, Kai; Getova, Valentina; Niemeyer, Verena; Zens, Katharina; Unnerstall, Tim R.; Feger, Julia S.; Fallah, Mohammad A.; Metze, Dieter; Ständer, Sonja; Luger, Thomas A.; Koenig, Karsten; Mess, Christian; Schneider, Stefan W.

    2016-03-01

    The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients’ bedsides. These ‘optical biopsies’ generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.

  10. Multiphoton imaging of low grade, high grade intraepithelial neoplasia and intramucosal invasive cancer of esophagus

    Science.gov (United States)

    Xu, Jian; Jiang, Liwei; Kang, Deyong; Wu, Xuejing; Xu, Meifang; Zhuo, Shuangmu; Zhu, Xiaoqin; Lin, Jiangbo; Chen, Jianxin

    2017-04-01

    Esophageal squamous cell carcinoma (ESCC) is devastating because of its aggressive lymphatic spread and clinical course. It is believed to occur through low-grade intraepithelial neoplasia (LGIN), high-grade intraepithelial neoplasia (HGIN), and intramucosal invasive cancer (IMC) before transforming to submucosal cancer. In particular, these early lesions (LGIN, HGIN and IMC), which involve no lymph node nor distant metastasis, can be cured by endoscopic treatment. Therefore, early identification of these lesions is important so as to offer a curative endoscopic resection, thus slowing down the development of ESCC. In this work, spectral information and morphological features of the normal esophageal mucosa are first studied. Then, the morphological changes of LGIN, HGIN and IMC are described. Lastly, quantitative parameters are also extracted by calculating the nuclear-to-cytoplasmic ratio of epithelial cells and the pixel density of collagen in the lamina propria. These results show that multiphoton microscopy (MPM) has the ability to identify normal esophageal mucosa, LGIN, HGIN and IMC. With the development of multiphoton endoscope systems for in vivo imaging, combined with a laser ablation system, MPM has the potential to provide immediate pathologic diagnosis and curative treatment of ESCC before the transformation to submucosal cancer in the future.

  11. Effects of point configuration on the accuracy in 3D reconstruction from biplane images

    International Nuclear Information System (INIS)

    Dmochowski, Jacek; Hoffmann, Kenneth R.; Singh, Vikas; Xu Jinhui; Nazareth, Daryl P.

    2005-01-01

    Two or more angiograms are being used frequently in medical imaging to reconstruct locations in three-dimensional (3D) space, e.g., for reconstruction of 3D vascular trees, implanted electrodes, or patient positioning. A number of techniques have been proposed for this task. In this simulation study, we investigate the effect of the shape of the configuration of the points in 3D (the 'cloud' of points) on reconstruction errors for one of these techniques developed in our laboratory. Five types of configurations (a ball, an elongated ellipsoid (cigar), flattened ball (pancake), flattened cigar, and a flattened ball with a single distant point) are used in the evaluations. For each shape, 100 random configurations were generated, with point coordinates chosen from Gaussian distributions having a covariance matrix corresponding to the desired shape. The 3D data were projected into the image planes using a known imaging geometry. Gaussian distributed errors were introduced in the x and y coordinates of these projected points. Gaussian distributed errors were also introduced into the gantry information used to calculate the initial imaging geometry. The imaging geometries and 3D positions were iteratively refined using the enhanced-Metz-Fencil technique. The image data were also used to evaluate the feasible R-t solution volume. The 3D errors between the calculated and true positions were determined. The effects of the shape of the configuration, the number of points, the initial geometry error, and the input image error were evaluated. The results for the number of points, initial geometry error, and image error are in agreement with previously reported results, i.e., increasing the number of points and reducing initial geometry and/or image error, improves the accuracy of the reconstructed data. The shape of the 3D configuration of points also affects the error of reconstructed 3D configuration; specifically, errors decrease as the 'volume' of the 3D configuration

  12. The Use of 3d City Models Form Oblique Images on Land Administration

    Science.gov (United States)

    Bakici, S.; Erkek, B.; Ayyildiz, E.; Özmüş, L.

    2017-11-01

    The article 718 of the civil law saying "The ownership on property includes the air above and terrain layers below to an extent providing benefit. The structures, plants and sources are included in the content of this ownership reserving the legal restrictions" and the cadastre law no. 3402 envisage 3D Cadastre. 3D data is required in order to perform 3D cadastre. To meet this requirement, oblique photogrammetry arises as the main data acquisition method. The data obtained by this method is used as base in 3D Cadastre and Land Administration activities. 3D cadastre required in the context of land administration activities in Turkey demands high resolution aerial oblique images to be used in services such as real estate value assessment & marketing in urban areas, urban planning, unlicensed construction monitoring & city administration and making location data (national address data etc.) intelligent.

  13. THE USE OF 3D CITY MODELS FORM OBLIQUE IMAGES ON LAND ADMINISTRATION

    Directory of Open Access Journals (Sweden)

    S. Bakici

    2017-11-01

    Full Text Available The article 718 of the civil law saying “The ownership on property includes the air above and terrain layers below to an extent providing benefit. The structures, plants and sources are included in the content of this ownership reserving the legal restrictions” and the cadastre law no. 3402 envisage 3D Cadastre. 3D data is required in order to perform 3D cadastre. To meet this requirement, oblique photogrammetry arises as the main data acquisition method. The data obtained by this method is used as base in 3D Cadastre and Land Administration activities. 3D cadastre required in the context of land administration activities in Turkey demands high resolution aerial oblique images to be used in services such as real estate value assessment & marketing in urban areas, urban planning, unlicensed construction monitoring & city administration and making location data (national address data etc. intelligent.

  14. The findings and the role of axial CT imaging and 3D imaging of gastric lesion by spiral CT

    International Nuclear Information System (INIS)

    Lee, Dong Ho; Ko, Young Tae

    1996-01-01

    The purpose of this study is to assess the efficacy of axial CT imaging and 3D imaging by spiral CT in the detection and evaluation of gastric lesion. Seventy-seven patients with pathologically-proven gastric lesions underwent axial CT and 3D imaging by spiral CT. There were 49 cases of advanced gastric carcinoma(AGC), 21 of early gastric carcinoma (EGC), three of benign ulcers, three of leiomyomas, and one case of lymphoma. Spiral CT was performed with 3-mm collimation, 4.5mm/sec table feed, and 1-1.5-mm reconstruction interval after the ingestion of gas. 3D imaging was obtained using the SSD technique, and on analysis a grade was given(excellent, good, poor). Axial CT scan was performed with 5-mm collimation, 7mm/sec table feed, and 5-mm reconstruction interval after the ingestion of water. Among 49 cases of AGC, excellent 3D images were obtained in seven patients (14.3%), good 3D images in 30(61.2%), and poor 3D images in 12(24.5%). Among the 12 patients with poor images, the cancers were located at the pyloric antrum in eight cases, were AGC Borrmann type 4 in three cases, and EGC-mimicking lesion in one case. Using axial CT scan alone, Borrmann's classification based tumor morphology were accurately identified in 67.3% of cases, but using 3D imaging, the corresponding figure was 85.7%. In 33 cases receiving surgery, good correlation between axial CT scan and pathology occurred in 72.7% of T class, and 69.7% of N class. Among 21 cases of EGC, excellent 3D images were obtained in three patients (14.3%), good 3D images in 14 (66.7%), and poor 3D images in two (9.5%). The other two cases of EGC were not detected. By axial CT scan, no tumor was detected in four cases, and there were two doubtful cases. 3D images of three benign ulcers were excellent in one case and good in two. 3D images of three leiomyomas and one lymphoma were excellent. Combined axial CT imaging and 3D imaging by spiral CT has the potential to accurately diagnose gastric lesions other than AGC

  15. Online 3D terrain visualisation using Unity 3D game engine: A comparison of different contour intervals terrain data draped with UAV images

    Science.gov (United States)

    Hafiz Mahayudin, Mohd; Che Mat, Ruzinoor

    2016-06-01

    The main objective of this paper is to discuss on the effectiveness of visualising terrain draped with Unmanned Aerial Vehicle (UAV) images generated from different contour intervals using Unity 3D game engine in online environment. The study area that was tested in this project was oil palm plantation at Sintok, Kedah. The contour data used for this study are divided into three different intervals which are 1m, 3m and 5m. ArcGIS software were used to clip the contour data and also UAV images data to be similar size for the overlaying process. The Unity 3D game engine was used as the main platform for developing the system due to its capabilities which can be launch in different platform. The clipped contour data and UAV images data were process and exported into the web format using Unity 3D. Then process continue by publishing it into the web server for comparing the effectiveness of different 3D terrain data (contour data) draped with UAV images. The effectiveness is compared based on the data size, loading time (office and out-of-office hours), response time, visualisation quality, and frame per second (fps). The results were suggest which contour interval is better for developing an effective online 3D terrain visualisation draped with UAV images using Unity 3D game engine. It therefore benefits decision maker and planner related to this field decide on which contour is applicable for their task.

  16. Single-breath-hold 3-D CINE imaging of the left ventricle using Cartesian sampling.

    Science.gov (United States)

    Wetzl, Jens; Schmidt, Michaela; Pontana, François; Longère, Benjamin; Lugauer, Felix; Maier, Andreas; Hornegger, Joachim; Forman, Christoph

    2018-02-01

    Our objectives were to evaluate a single-breath-hold approach for Cartesian 3-D CINE imaging of the left ventricle with a nearly isotropic resolution of [Formula: see text] and a breath-hold duration of [Formula: see text]19 s against a standard stack of 2-D CINE slices acquired in multiple breath-holds. Validation is performed with data sets from ten healthy volunteers. A Cartesian sampling pattern based on the spiral phyllotaxis and a compressed sensing reconstruction method are proposed to allow 3-D CINE imaging with high acceleration factors. The fully integrated reconstruction uses multiple graphics processing units to speed up the reconstruction. The 2-D CINE and 3-D CINE are compared based on ventricular function parameters, contrast-to-noise ratio and edge sharpness measurements. Visual comparisons of corresponding short-axis slices of 2-D and 3-D CINE show an excellent match, while 3-D CINE also allows reformatting to other orientations. Ventricular function parameters do not significantly differ from values based on 2-D CINE imaging. Reconstruction times are below 4 min. We demonstrate single-breath-hold 3-D CINE imaging in volunteers and three example patient cases, which features fast reconstruction and allows reformatting to arbitrary orientations.

  17. Imaging photoelectron circular dichroism of chiral molecules by femtosecond multiphoton coincidence detection

    Energy Technology Data Exchange (ETDEWEB)

    Lehmann, C. Stefan; Ram, N. Bhargava; Janssen, Maurice H. M., E-mail: m.h.m.janssen@vu.nl [LaserLaB Amsterdam, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam (Netherlands); Powis, Ivan [School of Chemistry, University of Nottingham, Nottingham NG7 2RD (United Kingdom)

    2013-12-21

    Here, we provide a detailed account of novel experiments employing electron-ion coincidence imaging to discriminate chiral molecules. The full three-dimensional angular scattering distribution of electrons is measured after photoexcitation with either left or right circular polarized light. The experiment is performed using a simplified photoelectron-photoion coincidence imaging setup employing only a single particle imaging detector. Results are reported applying this technique to enantiomers of the chiral molecule camphor after three-photon ionization by circularly polarized femtosecond laser pulses at 400 nm and 380 nm. The electron-ion coincidence imaging provides the photoelectron spectrum of mass-selected ions that are observed in the time-of-flight mass spectra. The coincident photoelectron spectra of the parent camphor ion and the various fragment ions are the same, so it can be concluded that fragmentation of camphor happens after ionization. We discuss the forward-backward asymmetry in the photoelectron angular distribution which is expressed in Legendre polynomials with moments up to order six. Furthermore, we present a method, similar to one-photon electron circular dichroism, to quantify the strength of the chiral electron asymmetry in a single parameter. The circular dichroism in the photoelectron angular distribution of camphor is measured to be 8% at 400 nm. The electron circular dichroism using femtosecond multiphoton excitation is of opposite sign and about 60% larger than the electron dichroism observed before in near-threshold one-photon ionization with synchrotron excitation. We interpret our multiphoton ionization as being resonant at the two-photon level with the 3s and 3p Rydberg states of camphor. Theoretical calculations are presented that model the photoelectron angular distribution from a prealigned camphor molecule using density functional theory and continuum multiple scattering X alpha photoelectron scattering calculations

  18. Comparative Accuracy of Facial Models Fabricated Using Traditional and 3D Imaging Techniques.

    Science.gov (United States)

    Lincoln, Ketu P; Sun, Albert Y T; Prihoda, Thomas J; Sutton, Alan J

    2016-04-01

    The purpose of this investigation was to compare the accuracy of facial models fabricated using facial moulage impression methods to the three-dimensional printed (3DP) fabrication methods using soft tissue images obtained from cone beam computed tomography (CBCT) and 3D stereophotogrammetry (3D-SPG) scans. A reference phantom model was fabricated using a 3D-SPG image of a human control form with ten fiducial markers placed on common anthropometric landmarks. This image was converted into the investigation control phantom model (CPM) using 3DP methods. The CPM was attached to a camera tripod for ease of image capture. Three CBCT and three 3D-SPG images of the CPM were captured. The DICOM and STL files from the three 3dMD and three CBCT were imported to the 3DP, and six testing models were made. Reversible hydrocolloid and dental stone were used to make three facial moulages of the CPM, and the impressions/casts were poured in type IV gypsum dental stone. A coordinate measuring machine (CMM) was used to measure the distances between each of the ten fiducial markers. Each measurement was made using one point as a static reference to the other nine points. The same measuring procedures were accomplished on all specimens. All measurements were compared between specimens and the control. The data were analyzed using ANOVA and Tukey pairwise comparison of the raters, methods, and fiducial markers. The ANOVA multiple comparisons showed significant difference among the three methods (p 3D-SPG showed statistical difference in comparison to the models fabricated using the traditional method of facial moulage and 3DP models fabricated from CBCT imaging. 3DP models fabricated using 3D-SPG were less accurate than the CPM and models fabricated using facial moulage and CBCT imaging techniques. © 2015 by the American College of Prosthodontists.

  19. Usefulness of 3D-VIBE method in breast dynamic MRI. Imaging parameters and contrasting effects

    International Nuclear Information System (INIS)

    Uchikoshi, Masato; Ueda, Takashi; Nishiki, Shigeo; Satou, Kouichi; Wada, Akihiko; Imaoka, Izumi; Matsuo, Michimasa

    2003-01-01

    MR imaging (MRI) has been reported to be a useful modality to characterize breast tumors and to evaluate disease extent. Contrast-enhanced dynamic MRI, in particular, allows breast lesions to be characterized with high sensitivity and specificity. Our study was designed to develop three-dimensional volumetric interpolated breath-hold examination (3D-VIBE) techniques for the evaluation of breast tumors. First, agarose/Gd-DTPA phantoms with various concentrations of Gd-DTPA were imaged using 3D-VIBE and turbo spin echo (TSE). Second, one of the phantoms was imaged with 3D-VIBE using different flip angles. Finally, water excitation (WE) and a chemical shift-selective (CHESS) pulse were applied to the images. Each image was analyzed for signal intensity, signal-to-noise ratio (1.25*Ms/Mb) (SNR), and contrast ratio [(Ms1-Ms2)/{(Ms1+Ms2)/2}]. The results showed that 3D-VIBE provided better contrast ratios with a linear fit than TSE, although 3D-VIBE showed a lower SNR. To reach the best contrast ratio, the optimized flip angle was found to be 30 deg for contrast-enhanced dynamic study. Both WE and CHESS pulses were reliable for obtaining fat- suppressed images. In conclusion, the 3D-VIBE technique can image the entire breast area with high resolution and provide better contrast than TSE. Our phantom study suggests that optimized 3D-VIBE may be useful for the assessment of breast tumors. (author)

  20. 3D-SIFT-Flow for atlas-based CT liver image segmentation

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Yan, E-mail: xuyan04@gmail.com [State Key Laboratory of Software Development Environment and Key Laboratory of Biomechanics and Mechanobiology of Ministry of Education, Beihang University, Beijing 100191, China and Research Institute of Beihang University in Shenzhen and Microsoft Research, Beijing 100080 (China); Xu, Chenchao, E-mail: chenchaoxu33@gmail.com; Kuang, Xiao, E-mail: kuangxiao.ace@gmail.com [School of Biological Science and Medical Engineering, Beihang University, Beijing 100191 (China); Wang, Hongkai, E-mail: wang.hongkai@gmail.com [Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024 (China); Chang, Eric I-Chao, E-mail: eric.chang@microsoft.com [Microsoft Research, Beijing 100080 (China); Huang, Weimin, E-mail: wmhuang@i2r.a-star.edu.sg [Institute for Infocomm Research (I2R), Singapore 138632 (Singapore); Fan, Yubo, E-mail: yubofan@buaa.edu.cn [Key Laboratory of Biomechanics and Mechanobiology of Ministry of Education, Beihang University, Beijing 100191 (China)

    2016-05-15

    Purpose: In this paper, the authors proposed a new 3D registration algorithm, 3D-scale invariant feature transform (SIFT)-Flow, for multiatlas-based liver segmentation in computed tomography (CT) images. Methods: In the registration work, the authors developed a new registration method that takes advantage of dense correspondence using the informative and robust SIFT feature. The authors computed the dense SIFT features for the source image and the target image and designed an objective function to obtain the correspondence between these two images. Labeling of the source image was then mapped to the target image according to the former correspondence, resulting in accurate segmentation. In the fusion work, the 2D-based nonparametric label transfer method was extended to 3D for fusing the registered 3D atlases. Results: Compared with existing registration algorithms, 3D-SIFT-Flow has its particular advantage in matching anatomical structures (such as the liver) that observe large variation/deformation. The authors observed consistent improvement over widely adopted state-of-the-art registration methods such as ELASTIX, ANTS, and multiatlas fusion methods such as joint label fusion. Experimental results of liver segmentation on the MICCAI 2007 Grand Challenge are encouraging, e.g., Dice overlap ratio 96.27% ± 0.96% by our method compared with the previous state-of-the-art result of 94.90% ± 2.86%. Conclusions: Experimental results show that 3D-SIFT-Flow is robust for segmenting the liver from CT images, which has large tissue deformation and blurry boundary, and 3D label transfer is effective and efficient for improving the registration accuracy.

  1. 3D-SIFT-Flow for atlas-based CT liver image segmentation.

    Science.gov (United States)

    Xu, Yan; Xu, Chenchao; Kuang, Xiao; Wang, Hongkai; Chang, Eric I-Chao; Huang, Weimin; Fan, Yubo

    2016-05-01

    In this paper, the authors proposed a new 3D registration algorithm, 3D-scale invariant feature transform (SIFT)-Flow, for multiatlas-based liver segmentation in computed tomography (CT) images. In the registration work, the authors developed a new registration method that takes advantage of dense correspondence using the informative and robust SIFT feature. The authors computed the dense SIFT features for the source image and the target image and designed an objective function to obtain the correspondence between these two images. Labeling of the source image was then mapped to the target image according to the former correspondence, resulting in accurate segmentation. In the fusion work, the 2D-based nonparametric label transfer method was extended to 3D for fusing the registered 3D atlases. Compared with existing registration algorithms, 3D-SIFT-Flow has its particular advantage in matching anatomical structures (such as the liver) that observe large variation/deformation. The authors observed consistent improvement over widely adopted state-of-the-art registration methods such as ELASTIX, ANTS, and multiatlas fusion methods such as joint label fusion. Experimental results of liver segmentation on the MICCAI 2007 Grand Challenge are encouraging, e.g., Dice overlap ratio 96.27% ± 0.96% by our method compared with the previous state-of-the-art result of 94.90% ± 2.86%. Experimental results show that 3D-SIFT-Flow is robust for segmenting the liver from CT images, which has large tissue deformation and blurry boundary, and 3D label transfer is effective and efficient for improving the registration accuracy.

  2. 3D-SIFT-Flow for atlas-based CT liver image segmentation

    International Nuclear Information System (INIS)

    Xu, Yan; Xu, Chenchao; Kuang, Xiao; Wang, Hongkai; Chang, Eric I-Chao; Huang, Weimin; Fan, Yubo

    2016-01-01

    Purpose: In this paper, the authors proposed a new 3D registration algorithm, 3D-scale invariant feature transform (SIFT)-Flow, for multiatlas-based liver segmentation in computed tomography (CT) images. Methods: In the registration work, the authors developed a new registration method that takes advantage of dense correspondence using the informative and robust SIFT feature. The authors computed the dense SIFT features for the source image and the target image and designed an objective function to obtain the correspondence between these two images. Labeling of the source image was then mapped to the target image according to the former correspondence, resulting in accurate segmentation. In the fusion work, the 2D-based nonparametric label transfer method was extended to 3D for fusing the registered 3D atlases. Results: Compared with existing registration algorithms, 3D-SIFT-Flow has its particular advantage in matching anatomical structures (such as the liver) that observe large variation/deformation. The authors observed consistent improvement over widely adopted state-of-the-art registration methods such as ELASTIX, ANTS, and multiatlas fusion methods such as joint label fusion. Experimental results of liver segmentation on the MICCAI 2007 Grand Challenge are encouraging, e.g., Dice overlap ratio 96.27% ± 0.96% by our method compared with the previous state-of-the-art result of 94.90% ± 2.86%. Conclusions: Experimental results show that 3D-SIFT-Flow is robust for segmenting the liver from CT images, which has large tissue deformation and blurry boundary, and 3D label transfer is effective and efficient for improving the registration accuracy.

  3. Flatbed-type 3D display systems using integral imaging method

    Science.gov (United States)

    Hirayama, Yuzo; Nagatani, Hiroyuki; Saishu, Tatsuo; Fukushima, Rieko; Taira, Kazuki

    2006-10-01

    We have developed prototypes of flatbed-type autostereoscopic display systems using one-dimensional integral imaging method. The integral imaging system reproduces light beams similar of those produced by a real object. Our display architecture is suitable for flatbed configurations because it has a large margin for viewing distance and angle and has continuous motion parallax. We have applied our technology to 15.4-inch displays. We realized horizontal resolution of 480 with 12 parallaxes due to adoption of mosaic pixel arrangement of the display panel. It allows viewers to see high quality autostereoscopic images. Viewing the display from angle allows the viewer to experience 3-D images that stand out several centimeters from the surface of the display. Mixed reality of virtual 3-D objects and real objects are also realized on a flatbed display. In seeking reproduction of natural 3-D images on the flatbed display, we developed proprietary software. The fast playback of the CG movie contents and real-time interaction are realized with the aid of a graphics card. Realization of the safety 3-D images to the human beings is very important. Therefore, we have measured the effects on the visual function and evaluated the biological effects. For example, the accommodation and convergence were measured at the same time. The various biological effects are also measured before and after the task of watching 3-D images. We have found that our displays show better results than those to a conventional stereoscopic display. The new technology opens up new areas of application for 3-D displays, including arcade games, e-learning, simulations of buildings and landscapes, and even 3-D menus in restaurants.

  4. Automatic segmentation of MRI head images by 3-D region growing method which utilizes edge information

    International Nuclear Information System (INIS)

    Jiang, Hao; Suzuki, Hidetomo; Toriwaki, Jun-ichiro

    1991-01-01

    This paper presents a 3-D segmentation method that automatically extracts soft tissue from multi-sliced MRI head images. MRI produces a sequence of two-dimensional (2-D) images which contains three-dimensional (3-D) information of organs. To utilize such information we need effective algorithms to treat 3-D digital images and to extract organs and tissues of interest. We developed a method to extract the brain from MRI images which uses a region growing procedure and integrates information of uniformity of gray levels and information of the presence of edge segments in the local area around the pixel of interest. First we generate a kernel region which is a part of brain tissue by simple thresholding. Then we grow the region by means of a region growing algorithm under the control of 3-D edge existence to obtain the region of the brain. Our method is rather simple because it uses basic 3-D image processing techniques like spatial difference. It is robust for variation of gray levels inside a tissue since it also refers to the edge information in the process of region growing. Therefore, the method is flexible enough to be applicable to the segmentation of other images including soft tissues which have complicated shapes and fluctuation in gray levels. (author)

  5. Periodic additive noises reduction in 3D images used in building of voxel phantoms through an efficient implementation of the 3D FFT: zipper artifacts filtering

    International Nuclear Information System (INIS)

    Oliveira, Alex C.H. de; Lima, Fernando R.A.; Vieira, Jose W.; Leal Neto, Viriato

    2009-01-01

    The anthropomorphic models used in computational dosimetry are predominantly build from scanning CT (Computed Tomography) or MRI (Magnetic Resonance Imaging) image stacks obtained of patients or volunteers. The building of these stacks (usually called of voxel phantoms or tomography phantoms) requires computer processing to be used in an exposure computational model. Noises present in these stacks can be confused with significant structures. In a 3D image with periodic additive noise in the frequency domain, the noise is fully added to its central slice. The discrete Fourier transform is the fundamental mathematical tool that allows the switch of the spatial domain for the frequency domain, and vice versa. The FFT (fast Fourier transform) algorithm is an ideal computational tool for this switch in domain with efficiency. This paper presents a new methodology for implementation in managed C++ language (Microsoft Visual Studio R .NET) of the fast Fourier transform of 3D digital images (FFT3D) using, essentially, the trigonometric recombination. The reduction of periodic additive noise consists in filtering only the central slice of 3D image in the frequency domain and transforms it back into the spatial domain through the inverse FFT3D. An example of application of this method it is the zipper artifacts filtering in images of MRI. These processes were implemented in the software DIP (Digital Image Processing). (author)

  6. Robotic 3D SQUID imaging system for practical nondestructive evaluation applications

    International Nuclear Information System (INIS)

    Isawa, K.; Nakayama, S.; Ikeda, M.; Takagi, S.; Tosaka, S.; Kasai, N.

    2005-01-01

    A robotic three-dimensional (3D) scanning superconducting quantum interference device (SQUID) imaging system was developed for practical nondestructive evaluation (NDE) applications. The major feature of this SQUID-NDE system is that the SQUID sensor itself scans in 3D by traveling over the surface of an object during testing without the need for magnetic shielding. This imaging system consists of (i) DC-SQUID gradiometer for effective movement of the sensor, (ii) SQUID sensor manipulator utilizing an articulated-type robot used in industry, (iii) laser charge-coupled-device (CCD) displacement sensor to measure the 3D coordinates of points on the surface of the object, and (iv) computer-aided numerical interpolation scheme for 3D surface reconstruction of the object. The applicability of this system for NDE was demonstrated by successfully detecting artificial damage of cylindrical-shaped steel tubes

  7. Detection of Connective Tissue Disorders from 3D Aortic MR Images Using Independent Component Analysis

    DEFF Research Database (Denmark)

    Hansen, Michael Sass; Zhao, Fei; Zhang, Honghai

    2006-01-01

    A computer-aided diagnosis (CAD) method is reported that allows the objective identification of subjects with connective tissue disorders from 3D aortic MR images using segmentation and independent component analysis (ICA). The first step to extend the model to 4D (3D + time) has also been taken....... ICA is an effective tool for connective tissue disease detection in the presence of sparse data using prior knowledge to order the components, and the components can be inspected visually. 3D+time MR image data sets acquired from 31 normal and connective tissue disorder subjects at end-diastole (R......-wave peak) and at 45\\$\\backslash\\$% of the R-R interval were used to evaluate the performance of our method. The automated 3D segmentation result produced accurate aortic surfaces covering the aorta. The CAD method distinguished between normal and connective tissue disorder subjects with a classification...

  8. Helical 3D-CT images of soft tissue tumors in the hand

    Energy Technology Data Exchange (ETDEWEB)

    Otani, Kazuhiro; Kikuchi, Hiraku; Tan, Akihiro; Hamanishi, Chiaki; Tanaka, Seisuke [Kinki Univ., Osaka-Sayama (Japan). School of Medicine

    2000-02-01

    X-ray, ultrasonograph CT, MRI and angiography are used to detect tumoral lesions. Recently, helical CT has been revealed to be a useful method for the diagnosis and preoperative evaluation of soft tissue tumors, by which high quality and accurate three dimensional (3D) images can be obtained quickly. We analyzed the preoperative 3D-CT images of soft tissue tumors in the hands of 11 cases (hemangioma in 6 cases, giant cell tumor, lipoma, angiofibroma, chondrosarcoma and malignant fibro-histiocytoma in one case each). Enhanced 3D-CT clearly visualized hemangiomas and solid tumors from the surrounding tissues. The tumors could easily be observed from any direction and color-coded according to the CT number. Helical 3D-CT was thus confirmed to be useful for the diagnosis and preoperative planning by indicating the details of tumor expansion into surrounding tissues. (author)

  9. An Algorithm for Fast Computation of 3D Zernike Moments for Volumetric Images

    OpenAIRE

    Hosny, Khalid M.; Hafez, Mohamed A.

    2012-01-01

    An algorithm was proposed for very fast and low-complexity computation of three-dimensional Zernike moments. The 3D Zernike moments were expressed in terms of exact 3D geometric moments where the later are computed exactly through the mathematical integration of the monomial terms over the digital image/object voxels. A new symmetry-based method was proposed to compute 3D Zernike moments with 87% reduction in the computational complexity. A fast 1D cascade algorithm was also employed to add m...

  10. 2D imaging and 3D sensing data acquisition and mutual registration for painting conservation

    Science.gov (United States)

    Fontana, Raffaella; Gambino, Maria Chiara; Greco, Marinella; Marras, Luciano; Pampaloni, Enrico M.; Pelagotti, Anna; Pezzati, Luca; Poggi, Pasquale

    2005-01-01

    We describe the application of 2D and 3D data acquisition and mutual registration to the conservation of paintings. RGB color image acquisition, IR and UV fluorescence imaging, together with the more recent hyperspectral imaging (32 bands) are among the most useful techniques in this field. They generally are meant to provide information on the painting materials, on the employed techniques and on the object state of conservation. However, only when the various images are perfectly registered on each other and on the 3D model, no ambiguity is possible and safe conclusions may be drawn. We present the integration of 2D and 3D measurements carried out on two different paintings: "Madonna of the Yarnwinder" by Leonardo da Vinci, and "Portrait of Lionello d'Este", by Pisanello, both painted in the XV century.

  11. Evaluation of 3D printing materials for fabrication of a novel multi-functional 3D thyroid phantom for medical dosimetry and image quality

    International Nuclear Information System (INIS)

    Alssabbagh, Moayyad; Tajuddin, Abd Aziz; Abdulmanap, Mahayuddin; Zainon, Rafidah

    2017-01-01

    Recently, the three-dimensional printer has started to be utilized strongly in medical industries. In the human body, many parts or organs can be printed from 3D images to meet accurate organ geometries. In this study, five common 3D printing materials were evaluated in terms of their elementary composition and the mass attenuation coefficients. The online version of XCOM photon cross-section database was used to obtain the attenuation values of each material. The results were compared with the attenuation values of the thyroid listed in the International Commission on Radiation Units and Measurements - ICRU 44. Two original thyroid models (hollow-inside and solid-inside) were designed from scratch to be used in nuclear medicine, diagnostic radiology and radiotherapy for dosimetry and image quality purposes. Both designs have three holes for installation of radiation dosimeters. The hollow-inside model has more two holes in the top for injection the radioactive materials. The attenuation properties of the Polylactic Acid (PLA) material showed a very good match with the thyroid tissue, which it was selected to 3D print the phantom using open source RepRap, Prusa i3 3D printer. The scintigraphy images show that the phantom simulates a real healthy thyroid gland and thus it can be used for image quality purposes. The measured CT numbers of the PA material after the 3D printing show a close match with the human thyroid CT numbers. Furthermore, the phantom shows a good accommodation of the TLD dosimeters inside the holes. The 3D fabricated thyroid phantom simulates the real shape of the human thyroid gland with a changeable geometrical shape-size feature to fit different age groups. By using 3D printing technology, the time required to fabricate the 3D phantom was considerably shortened compared to the longer conventional methods, where it took only 30 min to print out the model. The 3D printing material used in this study is commercially available and cost

  12. 3D Tendon Strain Estimation Using High-frequency Volumetric Ultrasound Images: A Feasibility Study.

    Science.gov (United States)

    Carvalho, Catarina; Slagmolen, Pieter; Bogaerts, Stijn; Scheys, Lennart; D'hooge, Jan; Peers, Koen; Maes, Frederik; Suetens, Paul

    2018-03-01

    Estimation of strain in tendons for tendinopathy assessment is a hot topic within the sports medicine community. It is believed that, if accurately estimated, existing treatment and rehabilitation protocols can be improved and presymptomatic abnormalities can be detected earlier. State-of-the-art studies present inaccurate and highly variable strain estimates, leaving this problem without solution. Out-of-plane motion, present when acquiring two-dimensional (2D) ultrasound (US) images, is a known problem and may be responsible for such errors. This work investigates the benefit of high-frequency, three-dimensional (3D) US imaging to reduce errors in tendon strain estimation. Volumetric US images were acquired in silico, in vitro, and ex vivo using an innovative acquisition approach that combines the acquisition of 2D high-frequency US images with a mechanical guided system. An affine image registration method was used to estimate global strain. 3D strain estimates were then compared with ground-truth values and with 2D strain estimates. The obtained results for in silico data showed a mean absolute error (MAE) of 0.07%, 0.05%, and 0.27% for 3D estimates along axial, lateral direction, and elevation direction and a respective MAE of 0.21% and 0.29% for 2D strain estimates. Although 3D could outperform 2D, this does not occur in in vitro and ex vivo settings, likely due to 3D acquisition artifacts. Comparison against the state-of-the-art methods showed competitive results. The proposed work shows that 3D strain estimates are more accurate than 2D estimates but acquisition of appropriate 3D US images remains a challenge.

  13. Intersection based motion correction of multislice MRI for 3-D in utero fetal brain image formation.

    Science.gov (United States)

    Kim, Kio; Habas, Piotr A; Rousseau, Francois; Glenn, Orit A; Barkovich, Anthony J; Studholme, Colin

    2010-01-01

    In recent years, postprocessing of fast multislice magnetic resonance imaging (MRI) to correct fetal motion has provided the first true 3-D MR images of the developing human brain in utero. Early approaches have used reconstruction based algorithms, employing a two-step iterative process, where slices from the acquired data are realigned to an approximate 3-D reconstruction of the fetal brain, which is then refined further using the improved slice alignment. This two step slice-to-volume process, although powerful, is computationally expensive in needing a 3-D reconstruction, and is limited in its ability to recover subvoxel alignment. Here, we describe an alternative approach which we term slice intersection motion correction (SIMC), that seeks to directly co-align multiple slice stacks by considering the matching structure along all intersecting slice pairs in all orthogonally planned slices that are acquired in clinical imaging studies. A collective update scheme for all slices is then derived, to simultaneously drive slices into a consistent match along their lines of intersection. We then describe a 3-D reconstruction algorithm that, using the final motion corrected slice locations, suppresses through-plane partial volume effects to provide a single high isotropic resolution 3-D image. The method is tested on simulated data with known motions and is applied to retrospectively reconstruct 3-D images from a range of clinically acquired imaging studies. The quantitative evaluation of the registration accuracy for the simulated data sets demonstrated a significant improvement over previous approaches. An initial application of the technique to studying clinical pathology is included, where the proposed method recovered up to 15 mm of translation and 30 degrees of rotation for individual slices, and produced full 3-D reconstructions containing clinically useful additional information not visible in the original 2-D slices.

  14. Procedure for making mannequins tailor for image quality control of PET by 3D printing systems

    International Nuclear Information System (INIS)

    Collado Chamorro, P. M.; Saez Beltran, F.; Diaz Pascual, V.; Benito Bejarado, M. A.; Sanz Freire, C. J.; Lopo Casqueiro, N.; Gonzalez Fernandez, M. P.; Lopez de Gamarra, M. S.

    2015-01-01

    There is a software free both for be the processes of modeling of the objects 3D to split of medical images, as for convert said modeling to file ready for be read and executed by the 3D printers (sequence or slicer). This lets make mannequins of Control of quality with a investment minimum. In this work is built a mannequin of brain refillable to measurement for be used in studies PET. (Author)

  15. MRI of the cartilages of the knee, 3-D imaging with a rapid computer system

    Energy Technology Data Exchange (ETDEWEB)

    Adam, G.; Bohndorf, K.; Prescher, A.; Drobnitzky, M.; Guenther, R.W.

    1989-01-01

    2-D spin-echo sequences were compared with 3-D gradient-echo sequences using normal and cadaver knee joints. The important advantages of 3-D-imaging are: sections of less than 1 mm, reconstruction in any required plane, which can be related to the complex anatomy of the knee joint, and very good distinction between intra-articular fluid, fibrocartilage and hyaline cartilage. (orig./GDG).

  16. A three-dimensional gradient refocused 3D volume imaging of discoid lateral meniscus

    International Nuclear Information System (INIS)

    Araki, Yutaka; Ootani, Masatoshi; Furukawa, Tomoaki; Yamamoto, Tadatsuka; Tomoda, Kaname; Tsukaguchi, Isao; Mitomo, Masanori.

    1991-01-01

    An axial 3D volume scan with MRI was applied to the evaluation of discoid lateral meniscus of the knee. By 0.7 mm-thick thin sliced and gapless images with volume scan, characteristically elongated appearance of discoid lateral meniscus was clearly depicted. These MR findings completely accorded with those on arthroscopy. Our conclusion is that an axial 3D volume scan was essential to the diagnosis of discoid lateral meniscus. (author)

  17. Curvature histogram features for retrieval of images of smooth 3D objects

    International Nuclear Information System (INIS)

    Zhdanov, I; Scherbakov, O; Potapov, A; Peterson, M

    2014-01-01

    We consider image features on the base of histograms of oriented gradients (HOG) with addition of contour curvature histogram (HOG-CH), and also compare it with results of known scale-invariant feature transform (SIFT) approach in application to retrieval of images of smooth 3D objects.

  18. Atlas-based mosaicing of 3D transesophageal echocardiography images of the left atrium

    NARCIS (Netherlands)

    Mulder, H.W. (Harriët); Pluim, J.P.W.; Ren, B. (Ben); Haak, A. (Alexander); Viergever, M.A. (Max); Bosch, J.G. (Johan); Stralen, van M. (Marijn)

    2015-01-01

    3D transesophageal echocardiography (TEE) is routinely used for planning and guidance of cardiac interventions. However, the limited field-of-view dictates the compounding of multiple images for visualization of large structures, e.g. the left atrium (LA). Previously, we developed a TEE image

  19. Automatic slice identification in 3D medical images with a ConvNet regressor

    NARCIS (Netherlands)

    de Vos, Bob D.; Viergever, Max A.; de Jong, Pim A.; Išgum, Ivana

    2016-01-01

    Identification of anatomical regions of interest is a prerequisite in many medical image analysis tasks. We propose a method that automatically identifies a slice of interest (SOI) in 3D images with a convolutional neural network (ConvNet) regressor. In 150 chest CT scans two reference slices were

  20. Simultaneous cell tracking and image alignment in 3D CLSM imagery of growing arabidopsis thaliana sepals

    NARCIS (Netherlands)

    Fick, R.H.J.; Fedorov, D.; Roeder, A.H.K.; Manjunath, B.S.

    2013-01-01

    In this research we propose a combined cell matching and image alignment method for tracking cells based on their nuclear locations in 3D fluorescent Confocal Laser Scanning Microscopy (CLSM) image sequences. We then apply it to study the cell division pattern in the developing sepal of the small

  1. Structured light 3D tracking system for measuring motions in PET brain imaging

    DEFF Research Database (Denmark)

    Olesen, Oline Vinter; Jørgensen, Morten Rudkjær; Paulsen, Rasmus Reinhold

    2010-01-01

    Patient motion during scanning deteriorates image quality, especially for high resolution PET scanners. A new proposal for a 3D head tracking system for motion correction in high resolution PET brain imaging is set up and demonstrated. A prototype tracking system based on structured light with a ...

  2. An Object-Oriented Simulator for 3D Digital Breast Tomosynthesis Imaging System

    Directory of Open Access Journals (Sweden)

    Saeed Seyyedi

    2013-01-01

    Full Text Available Digital breast tomosynthesis (DBT is an innovative imaging modality that provides 3D reconstructed images of breast to detect the breast cancer. Projections obtained with an X-ray source moving in a limited angle interval are used to reconstruct 3D image of breast. Several reconstruction algorithms are available for DBT imaging. Filtered back projection algorithm has traditionally been used to reconstruct images from projections. Iterative reconstruction algorithms such as algebraic reconstruction technique (ART were later developed. Recently, compressed sensing based methods have been proposed in tomosynthesis imaging problem. We have developed an object-oriented simulator for 3D digital breast tomosynthesis (DBT imaging system using C++ programming language. The simulator is capable of implementing different iterative and compressed sensing based reconstruction methods on 3D digital tomosynthesis data sets and phantom models. A user friendly graphical user interface (GUI helps users to select and run the desired methods on the designed phantom models or real data sets. The simulator has been tested on a phantom study that simulates breast tomosynthesis imaging problem. Results obtained with various methods including algebraic reconstruction technique (ART and total variation regularized reconstruction techniques (ART+TV are presented. Reconstruction results of the methods are compared both visually and quantitatively by evaluating performances of the methods using mean structural similarity (MSSIM values.

  3. An object-oriented simulator for 3D digital breast tomosynthesis imaging system.

    Science.gov (United States)

    Seyyedi, Saeed; Cengiz, Kubra; Kamasak, Mustafa; Yildirim, Isa

    2013-01-01

    Digital breast tomosynthesis (DBT) is an innovative imaging modality that provides 3D reconstructed images of breast to detect the breast cancer. Projections obtained with an X-ray source moving in a limited angle interval are used to reconstruct 3D image of breast. Several reconstruction algorithms are available for DBT imaging. Filtered back projection algorithm has traditionally been used to reconstruct images from projections. Iterative reconstruction algorithms such as algebraic reconstruction technique (ART) were later developed. Recently, compressed sensing based methods have been proposed in tomosynthesis imaging problem. We have developed an object-oriented simulator for 3D digital breast tomosynthesis (DBT) imaging system using C++ programming language. The simulator is capable of implementing different iterative and compressed sensing based reconstruction methods on 3D digital tomosynthesis data sets and phantom models. A user friendly graphical user interface (GUI) helps users to select and run the desired methods on the designed phantom models or real data sets. The simulator has been tested on a phantom study that simulates breast tomosynthesis imaging problem. Results obtained with various methods including algebraic reconstruction technique (ART) and total variation regularized reconstruction techniques (ART+TV) are presented. Reconstruction results of the methods are compared both visually and quantitatively by evaluating performances of the methods using mean structural similarity (MSSIM) values.

  4. Rigorous accuracy assessment for 3D reconstruction using time-series Dual Fluoroscopy (DF) image pairs

    Science.gov (United States)

    Al-Durgham, Kaleel; Lichti, Derek D.; Kuntze, Gregor; Ronsky, Janet

    2017-06-01

    High-speed biplanar videoradiography, or clinically referred to as dual fluoroscopy (DF), imaging systems are being used increasingly for skeletal kinematics analysis. Typically, a DF system comprises two X-ray sources, two image intensifiers and two high-speed video cameras. The combination of these elements provides time-series image pairs of articulating bones of a joint, which permits the measurement of bony rotation and translation in 3D at high temporal resolution (e.g., 120-250 Hz). Assessment of the accuracy of 3D measurements derived from DF imaging has been the subject of recent research efforts by several groups, however with methodological limitations. This paper presents a novel and simple accuracy assessment procedure based on using precise photogrammetric tools. We address the fundamental photogrammetry principles for the accuracy evaluation of an imaging system. Bundle adjustment with selfcalibration is used for the estimation of the system parameters. The bundle adjustment calibration uses an appropriate sensor model and applies free-network constraints and relative orientation stability constraints for a precise estimation of the system parameters. A photogrammetric intersection of time-series image pairs is used for the 3D reconstruction of a rotating planar object. A point-based registration method is used to combine the 3D coordinates from the intersection and independently surveyed coordinates. The final DF accuracy measure is reported as the distance between 3D coordinates from image intersection and the independently surveyed coordinates. The accuracy assessment procedure is designed to evaluate the accuracy over the full DF image format and a wide range of object rotation. Experiment of reconstruction of a rotating planar object reported an average positional error of 0.44 +/- 0.2 mm in the derived 3D coordinates (minimum 0.05 and maximum 1.2 mm).

  5. Parallax barrier engineering for image quality improvement in an autostereoscopic 3D display.

    Science.gov (United States)

    Kim, Sung-Kyu; Yoon, Ki-Hyuk; Yoon, Seon Kyu; Ju, Heongkyu

    2015-05-18

    We present a image quality improvement in a parallax barrier (PB)-based multiview autostereoscopic 3D display system under a real-time tracking of positions of a viewer's eyes. The system presented exploits a parallax barrier engineered to offer significantly improved quality of three-dimensional images for a moving viewer without an eyewear under the dynamic eye tracking. The improved image quality includes enhanced uniformity of image brightness, reduced point crosstalk, and no pseudoscopic effects. We control the relative ratio between two parameters i.e., a pixel size and the aperture of a parallax barrier slit to improve uniformity of image brightness at a viewing zone. The eye tracking that monitors positions of a viewer's eyes enables pixel data control software to turn on only pixels for view images near the viewer's eyes (the other pixels turned off), thus reducing point crosstalk. The eye tracking combined software provides right images for the respective eyes, therefore producing no pseudoscopic effects at its zone boundaries. The viewing zone can be spanned over area larger than the central viewing zone offered by a conventional PB-based multiview autostereoscopic 3D display (no eye tracking). Our 3D display system also provides multiviews for motion parallax under eye tracking. More importantly, we demonstrate substantial reduction of point crosstalk of images at the viewing zone, its level being comparable to that of a commercialized eyewear-assisted 3D display system. The multiview autostereoscopic 3D display presented can greatly resolve the point crosstalk problem, which is one of the critical factors that make it difficult for previous technologies for a multiview autostereoscopic 3D display to replace an eyewear-assisted counterpart.

  6. 3D stereotaxis for epileptic foci through integrating MR imaging with neurological electrophysiology data

    International Nuclear Information System (INIS)

    Luo Min; Peng Chenglin; Wang Kang; Lei Wenyong; Luo Song; Wang Xiaolin; Wang Xuejian; Wu Ruoqiu; Wu Guofeng

    2005-01-01

    Objective: To improve the accuracy of the epilepsy diagnoses by integrating MR image from PACS with data from neurological electrophysiology. The integration is also very important for transmiting diagnostic information to 3D TPS of radiotherapy. Methods: The electroencephalogram was redisplayed by EEG workstation, while MR image was reconstructed by Brainvoyager software. 3D model of patient brain was built up by combining reconstructed images with electroencephalogram data in Base 2000. 30 epileptic patients (18 males and 12 females) with their age ranged from 12 to 54 years were confirmed by using the integrated MR images and the data from neurological electrophysiology and their 3D stereolocating. Results: The corresponding data in 3D model could show the real situation of patients' brain and visually locate the precise position of the focus. The suddessful rate of 3D guided operation was greatly improved, and the number of epileptic onset was markedly decreased. The epilepsy was stopped for 6 months in 8 of the 30 patients. Conclusion: The integration of MR image and information of neurological electrophysiology can improve the diagnostic level for epilepsy, and it is crucial for imp roving the successful rate of manipulations and the epilepsy analysis. (authors)

  7. INTEGRATION OF VIDEO IMAGES AND CAD WIREFRAMES FOR 3D OBJECT LOCALIZATION

    Directory of Open Access Journals (Sweden)

    R. A. Persad

    2012-07-01

    Full Text Available The tracking of moving objects from single images has received widespread attention in photogrammetric computer vision and considered to be at a state of maturity. This paper presents a model-driven solution for localizing moving objects detected from monocular, rotating and zooming video images in a 3D reference frame. To realize such a system, the recovery of 2D to 3D projection parameters is essential. Automatic estimation of these parameters is critical, particularly for pan-tilt-zoom (PTZ surveillance cameras where parameters change spontaneously upon camera motion. In this work, an algorithm for automated parameter retrieval is proposed. This is achieved by matching linear features between incoming images from video sequences and simple geometric 3D CAD wireframe models of man-made structures. The feature matching schema uses a hypothesis-verify optimization framework referred to as LR-RANSAC. This novel method improves the computational efficiency of the matching process in comparison to the standard RANSAC robust estimator. To demonstrate the applicability and performance of the method, experiments have been performed on indoor and outdoor image sequences under varying conditions with lighting changes and occlusions. Reliability of the matching algorithm has been analyzed by comparing the automatically determined camera parameters with ground truth (GT. Dependability of the retrieved parameters for 3D localization has also been assessed by comparing the difference between 3D positions of moving image objects estimated using the LR-RANSAC-derived parameters and those computed using GT parameters.

  8. AUTOMATIC TEXTURE RECONSTRUCTION OF 3D CITY MODEL FROM OBLIQUE IMAGES

    Directory of Open Access Journals (Sweden)

    J. Kang

    2016-06-01

    Full Text Available In recent years, the photorealistic 3D city models are increasingly important in various geospatial applications related to virtual city tourism, 3D GIS, urban planning, real-estate management. Besides the acquisition of high-precision 3D geometric data, texture reconstruction is also a crucial step for generating high-quality and visually realistic 3D models. However, most of the texture reconstruction approaches are probably leading to texture fragmentation and memory inefficiency. In this paper, we introduce an automatic framework of texture reconstruction to generate textures from oblique images for photorealistic visualization. Our approach include three major steps as follows: mesh parameterization, texture atlas generation and texture blending. Firstly, mesh parameterization procedure referring to mesh segmentation and mesh unfolding is performed to reduce geometric distortion in the process of mapping 2D texture to 3D model. Secondly, in the texture atlas generation step, the texture of each segmented region in texture domain is reconstructed from all visible images with exterior orientation and interior orientation parameters. Thirdly, to avoid color discontinuities at boundaries between texture regions, the final texture map is generated by blending texture maps from several corresponding images. We evaluated our texture reconstruction framework on a dataset of a city. The resulting mesh model can get textured by created texture without resampling. Experiment results show that our method can effectively mitigate the occurrence of texture fragmentation. It is demonstrated that the proposed framework is effective and useful for automatic texture reconstruction of 3D city model.

  9. Analysis of scalability of high-performance 3D image processing platform for virtual colonoscopy.

    Science.gov (United States)

    Yoshida, Hiroyuki; Wu, Yin; Cai, Wenli

    2014-03-19

    One of the key challenges in three-dimensional (3D) medical imaging is to enable the fast turn-around time, which is often required for interactive or real-time response. This inevitably requires not only high computational power but also high memory bandwidth due to the massive amount of data that need to be processed. For this purpose, we previously developed a software platform for high-performance 3D medical image processing, called HPC 3D-MIP platform, which employs increasingly available and affordable commodity computing systems such as the multicore, cluster, and cloud computing systems. To achieve scalable high-performance computing, the platform employed size-adaptive, distributable block volumes as a core data structure for efficient parallelization of a wide range of 3D-MIP algorithms, supported task scheduling for efficient load distribution and balancing, and consisted of a layered parallel software libraries that allow image processing applications to share the common functionalities. We evaluated the performance of the HPC 3D-MIP platform by applying it to computationally intensive processes in virtual colonoscopy. Experimental results showed a 12-fold performance improvement on a workstation with 12-core CPUs over the original sequential implementation of the processes, indicating the efficiency of the platform. Analysis of performance scalability based on the Amdahl's law for symmetric multicore chips showed the potential of a high performance scalability of the HPC 3D-MIP platform when a larger number of cores is available.

  10. 3D Texture Analysis in Renal Cell Carcinoma Tissue Image Grading

    Science.gov (United States)

    Cho, Nam-Hoon; Choi, Heung-Kook

    2014-01-01

    One of the most significant processes in cancer cell and tissue image analysis is the efficient extraction of features for grading purposes. This research applied two types of three-dimensional texture analysis methods to the extraction of feature values from renal cell carcinoma tissue images, and then evaluated the validity of the methods statistically through grade classification. First, we used a confocal laser scanning microscope to obtain image slices of four grades of renal cell carcinoma, which were then reconstructed into 3D volumes. Next, we extracted quantitative values using a 3D gray level cooccurrence matrix (GLCM) and a 3D wavelet based on two types of basis functions. To evaluate their validity, we predefined 6 different statistical classifiers and applied these to the extracted feature sets. In the grade classification results, 3D Haar wavelet texture features combined with principal component analysis showed the best discrimination results. Classification using 3D wavelet texture features was significantly better than 3D GLCM, suggesting that the former has potential for use in a computer-based grading system. PMID:25371701

  11. 3D Texture Analysis in Renal Cell Carcinoma Tissue Image Grading

    Directory of Open Access Journals (Sweden)

    Tae-Yun Kim

    2014-01-01

    Full Text Available One of the most significant processes in cancer cell and tissue image analysis is the efficient extraction of features for grading purposes. This research applied two types of three-dimensional texture analysis methods to the extraction of feature values from renal cell carcinoma tissue images, and then evaluated the validity of the methods statistically through grade classification. First, we used a confocal laser scanning microscope to obtain image slices of four grades of renal cell carcinoma, which were then reconstructed into 3D volumes. Next, we extracted quantitative values using a 3D gray level cooccurrence matrix (GLCM and a 3D wavelet based on two types of basis functions. To evaluate their validity, we predefined 6 different statistical classifiers and applied these to the extracted feature sets. In the grade classification results, 3D Haar wavelet texture features combined with principal component analysis showed the best discrimination results. Classification using 3D wavelet texture features was significantly better than 3D GLCM, suggesting that the former has potential for use in a computer-based grading system.

  12. Externally calibrated parallel imaging for 3D multispectral imaging near metallic implants using broadband ultrashort echo time imaging.

    Science.gov (United States)

    Wiens, Curtis N; Artz, Nathan S; Jang, Hyungseok; McMillan, Alan B; Reeder, Scott B

    2017-06-01

    To develop an externally calibrated parallel imaging technique for three-dimensional multispectral imaging (3D-MSI) in the presence of metallic implants. A fast, ultrashort echo time (UTE) calibration acquisition is proposed to enable externally calibrated parallel imaging techniques near metallic implants. The proposed calibration acquisition uses a broadband radiofrequency (RF) pulse to excite the off-resonance induced by the metallic implant, fully phase-encoded imaging to prevent in-plane distortions, and UTE to capture rapidly decaying signal. The performance of the externally calibrated parallel imaging reconstructions was assessed using phantoms and in vivo examples. Phantom and in vivo comparisons to self-calibrated parallel imaging acquisitions show that significant reductions in acquisition times can be achieved using externally calibrated parallel imaging with comparable image quality. Acquisition time reductions are particularly large for fully phase-encoded methods such as spectrally resolved fully phase-encoded three-dimensional (3D) fast spin-echo (SR-FPE), in which scan time reductions of up to 8 min were obtained. A fully phase-encoded acquisition with broadband excitation and UTE enabled externally calibrated parallel imaging for 3D-MSI, eliminating the need for repeated calibration regions at each frequency offset. Significant reductions in acquisition time can be achieved, particularly for fully phase-encoded methods like SR-FPE. Magn Reson Med 77:2303-2309, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

  13. A 3D Image Filter for Parameter-Free Segmentation of Macromolecular Structures from Electron Tomograms

    Science.gov (United States)

    Ali, Rubbiya A.; Landsberg, Michael J.; Knauth, Emily; Morgan, Garry P.; Marsh, Brad J.; Hankamer, Ben

    2012-01-01

    3D image reconstruction of large cellular volumes by electron tomography (ET) at high (≤5 nm) resolution can now routinely resolve organellar and compartmental membrane structures, protein coats, cytoskeletal filaments, and macromolecules. However, current image analysis methods for identifying in situ macromolecular structures within the crowded 3D ultrastructural landscape of a cell remain labor-intensive, time-consuming, and prone to user-bias and/or error. This paper demonstrates the development and application of a parameter-free, 3D implementation of the bilateral edge-detection (BLE) algorithm for the rapid and accurate segmentation of cellular tomograms. The performance of the 3D BLE filter has been tested on a range of synthetic and real biological data sets and validated against current leading filters—the pseudo 3D recursive and Canny filters. The performance of the 3D BLE filter was found to be comparable to or better than that of both the 3D recursive and Canny filters while offering the significant advantage that it requires no parameter input or optimisation. Edge widths as little as 2 pixels are reproducibly detected with signal intensity and grey scale values as low as 0.72% above the mean of the background noise. The 3D BLE thus provides an efficient method for the automated segmentation of complex cellular structures across multiple scales for further downstream processing, such as cellular annotation and sub-tomogram averaging, and provides a valuable tool for the accurate and high-throughput identification and annotation of 3D structural complexity at the subcellular level, as well as for mapping the spatial and temporal rearrangement of macromolecular assemblies in situ within cellular tomograms. PMID:22479430

  14. Parametric modelling and segmentation of vertebral bodies in 3D CT and MR spine images

    International Nuclear Information System (INIS)

    Štern, Darko; Likar, Boštjan; Pernuš, Franjo; Vrtovec, Tomaž

    2011-01-01

    Accurate and objective evaluation of vertebral deformations is of significant importance in clinical diagnostics and therapy of pathological conditions affecting the spine. Although modern clinical practice is focused on three-dimensional (3D) computed tomography (CT) and magnetic resonance (MR) imaging techniques, the established methods for evaluation of vertebral deformations are limited to measuring deformations in two-dimensional (2D) x-ray images. In this paper, we propose a method for quantitative description of vertebral body deformations by efficient modelling and segmentation of vertebral bodies in 3D. The deformations are evaluated from the parameters of a 3D superquadric model, which is initialized as an elliptical cylinder and then gradually deformed by introducing transformations that yield a more detailed representation of the vertebral body shape. After modelling the vertebral body shape with 25 clinically meaningful parameters and the vertebral body pose with six rigid body parameters, the 3D model is aligned to the observed vertebral body in the 3D image. The performance of the method was evaluated on 75 vertebrae from CT and 75 vertebrae from T 2 -weighted MR spine images, extracted from the thoracolumbar part of normal and pathological spines. The results show that the proposed method can be used for 3D segmentation of vertebral bodies in CT and MR images, as the proposed 3D model is able to describe both normal and pathological vertebral body deformations. The method may therefore be used for initialization of whole vertebra segmentation or for quantitative measurement of vertebral body deformations.

  15. MO-C-18A-01: Advances in Model-Based 3D Image Reconstruction

    International Nuclear Information System (INIS)

    Chen, G; Pan, X; Stayman, J; Samei, E

    2014-01-01

    Recent years have seen the emergence of CT image reconstruction techniques that exploit physical models of the imaging system, photon statistics, and even the patient to achieve improved 3D image quality and/or reduction of radiation dose. With numerous advantages in comparison to conventional 3D filtered backprojection, such techniques bring a variety of challenges as well, including: a demanding computational load associated with sophisticated forward models and iterative optimization methods; nonlinearity and nonstationarity in image quality characteristics; a complex dependency on multiple free parameters; and the need to understand how best to incorporate prior information (including patient-specific prior images) within the reconstruction process. The advantages, however, are even greater – for example: improved image quality; reduced dose; robustness to noise and artifacts; task-specific reconstruction protocols; suitability to novel CT imaging platforms and noncircular orbits; and incorporation of known characteristics of the imager and patient that are conventionally discarded. This symposium features experts in 3D image reconstruction, image quality assessment, and the translation of such methods to emerging clinical applications. Dr. Chen will address novel methods for the incorporation of prior information in 3D and 4D CT reconstruction techniques. Dr. Pan will show recent advances in optimization-based reconstruction that enable potential reduction of dose and sampling requirements. Dr. Stayman will describe a “task-based imaging” approach that leverages models of the imaging system and patient in combination with a specification of the imaging task to optimize both the acquisition and reconstruction process. Dr. Samei will describe the development of methods for image quality assessment in such nonlinear reconstruction techniques and the use of these methods to characterize and optimize image quality and dose in a spectrum of clinical

  16. 3D T2-weighted imaging to shorten multiparametric prostate MRI protocols.

    Science.gov (United States)

    Polanec, Stephan H; Lazar, Mathias; Wengert, Georg J; Bickel, Hubert; Spick, Claudio; Susani, Martin; Shariat, Shahrokh; Clauser, Paola; Baltzer, Pascal A T

    2018-04-01

    To determine whether 3D acquisitions provide equivalent image quality, lesion delineation quality and PI-RADS v2 performance compared to 2D acquisitions in T2-weighted imaging of the prostate at 3 T. This IRB-approved, prospective study included 150 consecutive patients (mean age 63.7 years, 35-84 years; mean PSA 7.2 ng/ml, 0.4-31.1 ng/ml). Two uroradiologists (R1, R2) independently rated image quality and lesion delineation quality using a five-point ordinal scale and assigned a PI-RADS score for 2D and 3D T2-weighted image data sets. Data were compared using visual grading characteristics (VGC) and receiver operating characteristics (ROC)/area under the curve (AUC) analysis. Image quality was similarly good to excellent for 2D T2w (mean score R1, 4.3 ± 0.81; R2, 4.7 ± 0.83) and 3D T2w (mean score R1, 4.3 ± 0.82; R2, 4.7 ± 0.69), p = 0.269. Lesion delineation was rated good to excellent for 2D (mean score R1, 4.16 ± 0.81; R2, 4.19 ± 0.92) and 3D T2w (R1, 4.19 ± 0.94; R2, 4.27 ± 0.94) without significant differences (p = 0.785). ROC analysis showed an equivalent performance for 2D (AUC 0.580-0.623) and 3D (AUC 0.576-0.629) T2w (p > 0.05, respectively). Three-dimensional acquisitions demonstrated equivalent image and lesion delineation quality, and PI-RADS v2 performance, compared to 2D in T2-weighted imaging of the prostate. Three-dimensional T2-weighted imaging could be used to considerably shorten prostate MRI protocols in clinical practice. • 3D shows equivalent image quality and lesion delineation compared to 2D T2w. • 3D T2w and 2D T2w image acquisition demonstrated comparable diagnostic performance. • Using a single 3D T2w acquisition may shorten the protocol by 40%. • Combined with short DCE, multiparametric protocols of 10 min are feasible.

  17. A novel image encryption algorithm based on a 3D chaotic map

    Science.gov (United States)

    Kanso, A.; Ghebleh, M.

    2012-07-01

    Recently [Solak E, Çokal C, Yildiz OT Biyikoǧlu T. Cryptanalysis of Fridrich's chaotic image encryption. Int J Bifur Chaos 2010;20:1405-1413] cryptanalyzed the chaotic image encryption algorithm of [Fridrich J. Symmetric ciphers based on two-dimensional chaotic maps. Int J Bifur Chaos 1998;8(6):1259-1284], which was considered a benchmark for measuring security of many image encryption algorithms. This attack can also be applied to other encryption algorithms that have a structure similar to Fridrich's algorithm, such as that of [Chen G, Mao Y, Chui, C. A symmetric image encryption scheme based on 3D chaotic cat maps. Chaos Soliton Fract 2004;21:749-761]. In this paper, we suggest a novel image encryption algorithm based on a three dimensional (3D) chaotic map that can defeat the aforementioned attack among other existing attacks. The design of the proposed algorithm is simple and efficient, and based on three phases which provide the necessary properties for a secure image encryption algorithm including the confusion and diffusion properties. In phase I, the image pixels are shuffled according to a search rule based on the 3D chaotic map. In phases II and III, 3D chaotic maps are used to scramble shuffled pixels through mixing and masking rules, respectively. Simulation results show that the suggested algorithm satisfies the required performance tests such as high level security, large key space and acceptable encryption speed. These characteristics make it a suitable candidate for use in cryptographic applications.

  18. Common crus aplasia: diagnosis by 3D volume rendering imaging using 3DFT-CISS sequence

    International Nuclear Information System (INIS)

    Kim, H.J.; Song, J.W.; Chon, K.-M.; Goh, E.-K.

    2004-01-01

    AIM: The purpose of this study was to evaluate the findings of three-dimensional (3D) volume rendering (VR) imaging in common crus aplasia (CCA) of the inner ear. MATERIALS AND METHODS: Using 3D VR imaging of temporal bone constructive interference in steady state (CISS) magnetic resonance (MR) images, we retrospectively reviewed seven inner ears of six children who were candidates for cochlear implants and who had been diagnosed with CCA. As controls, we used the same method to examine 402 inner ears of 201 patients who had no clinical symptoms or signs of sensorineural hearing loss. Temporal bone MR imaging (MRI) was performed with a 1.5 T MR machine using a CISS sequence, and VR of the inner ear was performed on a work station. Morphological image analysis was performed on rotation views of 3D VR images. RESULTS: In all seven cases, CCA was diagnosed by the absence of the common crus. The remaining superior semicircular canal (SCC) was normal in five and hypoplastic in two inner ears, while the posterior SCC was normal in all seven. One patient showed bilateral symmetrical CCA. Complicated combined anomalies were seen in the cochlea, vestibule and lateral SCC. CONCLUSION: 3D VR imaging findings with MR CISS sequence can directly diagnose CCA. This technique may be useful in delineating detailed anomalies of SCCs

  19. Subnuclear foci quantification using high-throughput 3D image cytometry

    Science.gov (United States)

    Wadduwage, Dushan N.; Parrish, Marcus; Choi, Heejin; Engelward, Bevin P.; Matsudaira, Paul; So, Peter T. C.

    2015-07-01

    Ionising radiation causes various types of DNA damages including double strand breaks (DSBs). DSBs are often recognized by DNA repair protein ATM which forms gamma-H2AX foci at the site of the DSBs that can be visualized using immunohistochemistry. However most of such experiments are of low throughput in terms of imaging and image analysis techniques. Most of the studies still use manual counting or classification. Hence they are limited to counting a low number of foci per cell (5 foci per nucleus) as the quantification process is extremely labour intensive. Therefore we have developed a high throughput instrumentation and computational pipeline specialized for gamma-H2AX foci quantification. A population of cells with highly clustered foci inside nuclei were imaged, in 3D with submicron resolution, using an in-house developed high throughput image cytometer. Imaging speeds as high as 800 cells/second in 3D were achieved by using HiLo wide-field depth resolved imaging and a remote z-scanning technique. Then the number of foci per cell nucleus were quantified using a 3D extended maxima transform based algorithm. Our results suggests that while most of the other 2D imaging and manual quantification studies can count only up to about 5 foci per nucleus our method is capable of counting more than 100. Moreover we show that 3D analysis is significantly superior compared to the 2D techniques.

  20. Audiovisual biofeedback improves image quality and reduces scan time for respiratory-gated 3D MRI

    Science.gov (United States)

    Lee, D.; Greer, P. B.; Arm, J.; Keall, P.; Kim, T.

    2014-03-01

    The purpose of this study was to test the hypothesis that audiovisual (AV) biofeedback can improve image quality and reduce scan time for respiratory-gated 3D thoracic MRI. For five healthy human subjects respiratory motion guidance in MR scans was provided using an AV biofeedback system, utilizing real-time respiratory motion signals. To investigate the improvement of respiratory-gated 3D MR images between free breathing (FB) and AV biofeedback (AV), each subject underwent two imaging sessions. Respiratory-related motion artifacts and imaging time were qualitatively evaluated in addition to the reproducibility of external (abdominal) motion. In the results, 3D MR images in AV biofeedback showed more anatomic information such as a clear distinction of diaphragm, lung lobes and sharper organ boundaries. The scan time was reduced from 401±215 s in FB to 334±94 s in AV (p-value 0.36). The root mean square variation of the displacement and period of the abdominal motion was reduced from 0.4±0.22 cm and 2.8±2.5 s in FB to 0.1±0.15 cm and 0.9±1.3 s in AV (p-value of displacement audiovisual biofeedback improves image quality and reduces scan time for respiratory-gated 3D MRI. These results suggest that AV biofeedback has the potential to be a useful motion management tool in medical imaging and radiation therapy procedures.

  1. Image denoising by sparse 3-D transform-domain collaborative filtering.

    Science.gov (United States)

    Dabov, Kostadin; Foi, Alessandro; Katkovnik, Vladimir; Egiazarian, Karen

    2007-08-01

    We propose a novel image denoising strategy based on an enhanced sparse representation in transform domain. The enhancement of the sparsity is achieved by grouping similar 2-D image fragments (e.g., blocks) into 3-D data arrays which we call "groups." Collaborative filtering is a special procedure developed to deal with these 3-D groups. We realize it using the three successive steps: 3-D transformation of a group, shrinkage of the transform spectrum, and inverse 3-D transformation. The result is a 3-D estimate that consists of the jointly filtered grouped image blocks. By attenuating the noise, the collaborative filtering reveals even the finest details shared by grouped blocks and, at the same time, it preserves the essential unique features of each individual block. The filtered blocks are then returned to their original positions. Because these blocks are overlapping, for each pixel, we obtain many different estimates which need to be combined. Aggregation is a particular averaging procedure which is exploited to take advantage of this redundancy. A significant improvement is obtained by a specially developed collaborative Wiener filtering. An algorithm based on this novel denoising strategy and its efficient implementation are presented in full detail; an extension to color-image denoising is also developed. The experimental results demonstrate that this computationally scalable algorithm achieves state-of-the-art denoising performance in terms of both peak signal-to-noise ratio and subjective visual quality.

  2. Using 3D printing techniques to create an anthropomorphic thorax phantom for medical imaging purposes.

    Science.gov (United States)

    Hazelaar, Colien; van Eijnatten, Maureen; Dahele, Max; Wolff, Jan; Forouzanfar, Tymour; Slotman, Ben; Verbakel, Wilko F A R

    2018-01-01

    Imaging phantoms are widely used for testing and optimization of imaging devices without the need to expose humans to irradiation. However, commercially available phantoms are commonly manufactured in simple, generic forms and sizes and therefore do not resemble the clinical situation for many patients. Using 3D printing techniques, we created a life-size phantom based on a clinical CT scan of the thorax from a patient with lung cancer. It was assembled from bony structures printed in gypsum, lung structures consisting of airways, blood vessels >1 mm, and outer lung surface, three lung tumors printed in nylon, and soft tissues represented by silicone (poured into a 3D-printed mold). Kilovoltage x-ray and CT images of the phantom closely resemble those of the real patient in terms of size, shapes, and structures. Surface comparison using 3D models obtained from the phantom and the 3D models used for printing showed mean differences 3D printing and molding techniques. The phantom closely resembles a real patient in terms of spatial accuracy and is currently being used to evaluate x-ray-based imaging quality and positional verification techniques for radiotherapy. © 2017 American Association of Physicists in Medicine.

  3. Pancreatic cancer cell detection by targeted lipid microbubbles and multiphoton imaging

    Science.gov (United States)

    Cromey, Benjamin; McDaniel, Ashley; Matsunaga, Terry; Vagner, Josef; Kieu, Khanh Quoc; Banerjee, Bhaskar

    2018-04-01

    Surgical resection of pancreatic cancer represents the only chance of cure and long-term survival in this common disease. Unfortunately, determination of a cancer-free margin at surgery is based on one or two tiny frozen section biopsies, which is far from ideal. Not surprisingly, cancer is usually left behind and is responsible for metastatic disease. We demonstrate a method of receptor-targeted imaging using peptide ligands, lipid microbubbles, and multiphoton microscopy that could lead to a fast and accurate way of examining the entire cut surface during surgery. Using a plectin-targeted microbubble, we performed a blinded in-vitro study to demonstrate avid binding of targeted microbubbles to pancreatic cancer cells but not noncancerous cell lines. Further work should lead to a much-needed point-of-care diagnostic test for determining clean margins in oncologic surgery.

  4. Two efficient label-equivalence-based connected-component labeling algorithms for 3-D binary images.

    Science.gov (United States)

    He, Lifeng; Chao, Yuyan; Suzuki, Kenji

    2011-08-01

    Whenever one wants to distinguish, recognize, and/or measure objects (connected components) in binary images, labeling is required. This paper presents two efficient label-equivalence-based connected-component labeling algorithms for 3-D binary images. One is voxel based and the other is run based. For the voxel-based one, we present an efficient method of deciding the order for checking voxels in the mask. For the run-based one, instead of assigning each foreground voxel, we assign each run a provisional label. Moreover, we use run data to label foreground voxels without scanning any background voxel in the second scan. Experimental results have demonstrated that our voxel-based algorithm is efficient for 3-D binary images with complicated connected components, that our run-based one is efficient for those with simple connected components, and that both are much more efficient than conventional 3-D labeling algorithms.

  5. Extracting 3D Parametric Curves from 2D Images of Helical Objects.

    Science.gov (United States)

    Willcocks, Chris G; Jackson, Philip T G; Nelson, Carl J; Obara, Boguslaw

    2017-09-01

    Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and the curve is validated against both synthetic and real-world data. The results show that the extracted 3D curve comes within close Hausdorff distance to the ground truth, and has near identical tortuosity for helical objects with a circular profile. Parameter insensitivity and robustness against high levels of image noise are demonstrated thoroughly and quantitatively.

  6. GPU acceleration towards real-time image reconstruction in 3D tomographic diffractive microscopy

    Science.gov (United States)

    Bailleul, J.; Simon, B.; Debailleul, M.; Liu, H.; Haeberlé, O.

    2012-06-01

    Phase microscopy techniques regained interest in allowing for the observation of unprepared specimens with excellent temporal resolution. Tomographic diffractive microscopy is an extension of holographic microscopy which permits 3D observations with a finer resolution than incoherent light microscopes. Specimens are imaged by a series of 2D holograms: their accumulation progressively fills the range of frequencies of the specimen in Fourier space. A 3D inverse FFT eventually provides a spatial image of the specimen. Consequently, acquisition then reconstruction are mandatory to produce an image that could prelude real-time control of the observed specimen. The MIPS Laboratory has built a tomographic diffractive microscope with an unsurpassed 130nm resolution but a low imaging speed - no less than one minute. Afterwards, a high-end PC reconstructs the 3D image in 20 seconds. We now expect an interactive system providing preview images during the acquisition for monitoring purposes. We first present a prototype implementing this solution on CPU: acquisition and reconstruction are tied in a producer-consumer scheme, sharing common data into CPU memory. Then we present a prototype dispatching some reconstruction tasks to GPU in order to take advantage of SIMDparallelization for FFT and higher bandwidth for filtering operations. The CPU scheme takes 6 seconds for a 3D image update while the GPU scheme can go down to 2 or > 1 seconds depending on the GPU class. This opens opportunities for 4D imaging of living organisms or crystallization processes. We also consider the relevance of GPU for 3D image interaction in our specific conditions.

  7. 3D change detection at street level using mobile laser scanning point clouds and terrestrial images

    Science.gov (United States)

    Qin, Rongjun; Gruen, Armin

    2014-04-01

    Automatic change detection and geo-database updating in the urban environment are difficult tasks. There has been much research on detecting changes with satellite and aerial images, but studies have rarely been performed at the street level, which is complex in its 3D geometry. Contemporary geo-databases include 3D street-level objects, which demand frequent data updating. Terrestrial images provides rich texture information for change detection, but the change detection with terrestrial images from different epochs sometimes faces problems with illumination changes, perspective distortions and unreliable 3D geometry caused by the lack of performance of automatic image matchers, while mobile laser scanning (MLS) data acquired from different epochs provides accurate 3D geometry for change detection, but is very expensive for periodical acquisition. This paper proposes a new method for change detection at street level by using combination of MLS point clouds and terrestrial images: the accurate but expensive MLS data acquired from an early epoch serves as the reference, and terrestrial images or photogrammetric images captured from an image-based mobile mapping system (MMS) at a later epoch are used to detect the geometrical changes between different epochs. The method will automatically mark the possible changes in each view, which provides a cost-efficient method for frequent data updating. The methodology is divided into several steps. In the first step, the point clouds are recorded by the MLS system and processed, with data cleaned and classified by semi-automatic means. In the second step, terrestrial images or mobile mapping images at a later epoch are taken and registered to the point cloud, and then point clouds are projected on each image by a weighted window based z-buffering method for view dependent 2D triangulation. In the next step, stereo pairs of the terrestrial images are rectified and re-projected between each other to check the geometrical

  8. Development of 3D printing system for human bone model manufacturing using medical images

    International Nuclear Information System (INIS)

    Oh, Wang Kyun

    2017-01-01

    The 3D printing selective laser sintering (SLS) and stereo lithography apparatus (SLA) method used for bone model production has good precision and resolution, but the printers are expensive and need professional knowledge for operation. The program that converts computed tomography digital imaging and communications in medicine (DICOM) file into STL (stereolithography) file is also expensive so requesting 3D printing companies takes a lot of time and cost, which is why they are not generally utilized in surgery. To produce bone models of fractured patients, the use of 3D imaging conversion program and 3D printing system should be convenient, and the cost of device and operation should be low. Besides, they should be able to produce big size bone models for application to surgery. Therefore, by using an fused deposition modeling (FDM) method 3D printer that uses thermoplastic materials such as DICOM Viewer OsiriX and plastic wires, this study developed 3D printing system for Fracture surgery Patients customized bone model production for many clinics to use for surgery of fracture patients by universalizing with no limit in printing sizes and low maintenance and production cost. It is expected to be widely applied to the overall areas of orthopedics' education, research and clinic. It is also expected to be conveniently used in not only university hospitals but also regular general hospitals

  9. Development of 3D printing system for human bone model manufacturing using medical images

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Wang Kyun [Dept. of Radiology, Chungcheongbuk-do Cheongju Medical Center, Cheongju (Korea, Republic of)

    2017-09-15

    The 3D printing selective laser sintering (SLS) and stereo lithography apparatus (SLA) method used for bone model production has good precision and resolution, but the printers are expensive and need professional knowledge for operation. The program that converts computed tomography digital imaging and communications in medicine (DICOM) file into STL (stereolithography) file is also expensive so requesting 3D printing companies takes a lot of time and cost, which is why they are not generally utilized in surgery. To produce bone models of fractured patients, the use of 3D imaging conversion program and 3D printing system should be convenient, and the cost of device and operation should be low. Besides, they should be able to produce big size bone models for application to surgery. Therefore, by using an fused deposition modeling (FDM) method 3D printer that uses thermoplastic materials such as DICOM Viewer OsiriX and plastic wires, this study developed 3D printing system for Fracture surgery Patients customized bone model production for many clinics to use for surgery of fracture patients by universalizing with no limit in printing sizes and low maintenance and production cost. It is expected to be widely applied to the overall areas of orthopedics' education, research and clinic. It is also expected to be conveniently used in not only university hospitals but also regular general hospitals.

  10. Measurement of facial soft tissues thickness using 3D computed tomographic images

    International Nuclear Information System (INIS)

    Jeong, Ho Gul; Kim, Kee Deog; Shin, Dong Won; Hu, Kyung Seok; Lee, Jae Bum; Park, Hyok; Park, Chang Seo; Han, Seung Ho

    2006-01-01

    To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side. The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. There were no statistically significant differences between the direct measurements and those using the 3D images(p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissue thickness more easily in forensic science and anthropology

  11. Measurement of facial soft tissues thickness using 3D computed tomographic images

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Ho Gul; Kim, Kee Deog; Shin, Dong Won; Hu, Kyung Seok; Lee, Jae Bum; Park, Hyok; Park, Chang Seo [Yonsei Univ. Hospital, Seoul (Korea, Republic of); Han, Seung Ho [Catholic Univ. of Korea, Seoul (Korea, Republic of)

    2006-03-15

    To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side. The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. There were no statistically significant differences between the direct measurements and those using the 3D images(p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissue thickness more easily in forensic science and anthropology.

  12. Small SWAP 3D imaging flash ladar for small tactical unmanned air systems

    Science.gov (United States)

    Bird, Alan; Anderson, Scott A.; Wojcik, Michael; Budge, Scott E.

    2015-05-01

    The Space Dynamics Laboratory (SDL), working with Naval Research Laboratory (NRL) and industry leaders Advanced Scientific Concepts (ASC) and Hood Technology Corporation, has developed a small SWAP (size, weight, and power) 3D imaging flash ladar (LAser Detection And Ranging) sensor system concept design for small tactical unmanned air systems (STUAS). The design utilizes an ASC 3D flash ladar camera and laser in a Hood Technology gyro-stabilized gimbal system. The design is an autonomous, intelligent, geo-aware sensor system that supplies real-time 3D terrain and target images. Flash ladar and visible camera data are processed at the sensor using a custom digitizer/frame grabber with compression. Mounted in the aft housing are power, controls, processing computers, and GPS/INS. The onboard processor controls pointing and handles image data, detection algorithms and queuing. The small SWAP 3D imaging flash ladar sensor system generates georeferenced terrain and target images with a low probability of false return and system SWAP estimate of system is modeled using LadarSIM, a MATLAB® and Simulink®- based ladar system simulator designed and developed by the Center for Advanced Imaging Ladar (CAIL) at Utah State University. We will present the concept design and modeled performance predictions.

  13. Recent advances in Optical Computed Tomography (OCT) imaging system for three dimensional (3D) radiotherapy dosimetry

    Science.gov (United States)

    Rahman, Ahmad Taufek Abdul; Farah Rosli, Nurul; Zain, Shafirah Mohd; Zin, Hafiz M.

    2018-01-01

    Radiotherapy delivery techniques for cancer treatment are becoming more complex and highly focused, to enable accurate radiation dose delivery to the cancerous tissue and minimum dose to the healthy tissue adjacent to tumour. Instrument to verify the complex dose delivery in radiotherapy such as optical computed tomography (OCT) measures the dose from a three-dimensional (3D) radiochromic dosimeter to ensure the accuracy of the radiotherapy beam delivery to the patient. OCT measures the optical density in radiochromic material that changes predictably upon exposure to radiotherapy beams. OCT systems have been developed using a photodiode and charged coupled device (CCD) as the detector. The existing OCT imaging systems have limitation in terms of the accuracy and the speed of the measurement. Advances in on-pixel intelligence CMOS image sensor (CIS) will be exploited in this work to replace current detector in OCT imaging systems. CIS is capable of on-pixel signal processing at a very fast imaging speed (over several hundred images per second) that will allow improvement in the 3D measurement of the optical density. The paper will review 3D radiochromic dosimeters and OCT systems developed and discuss how CMOS based OCT imaging will provide accurate and fast optical density measurements in 3D. The paper will also discuss the configuration of the CMOS based OCT developed in this work and how it may improve the existing OCT system.

  14. 3D nonrigid medical image registration using a new information theoretic measure

    Science.gov (United States)

    Li, Bicao; Yang, Guanyu; Coatrieux, Jean Louis; Li, Baosheng; Shu, Huazhong

    2015-11-01

    This work presents a novel method for the nonrigid registration of medical images based on the Arimoto entropy, a generalization of the Shannon entropy. The proposed method employed the Jensen-Arimoto divergence measure as a similarity metric to measure the statistical dependence between medical images. Free-form deformations were adopted as the transformation model and the Parzen window estimation was applied to compute the probability distributions. A penalty term is incorporated into the objective function to smooth the nonrigid transformation. The goal of registration is to optimize an objective function consisting of a dissimilarity term and a penalty term, which would be minimal when two deformed images are perfectly aligned using the limited memory BFGS optimization method, and thus to get the optimal geometric transformation. To validate the performance of the proposed method, experiments on both simulated 3D brain MR images and real 3D thoracic CT data sets were designed and performed on the open source elastix package. For the simulated experiments, the registration errors of 3D brain MR images with various magnitudes of known deformations and different levels of noise were measured. For the real data tests, four data sets of 4D thoracic CT from four patients were selected to assess the registration performance of the method, including ten 3D CT images for each 4D CT data covering an entire respiration cycle. These results were compared with the normalized cross correlation and the mutual information methods and show a slight but true improvement in registration accuracy.

  15. 3D nonrigid medical image registration using a new information theoretic measure

    International Nuclear Information System (INIS)

    Li, Bicao; Yang, Guanyu; Coatrieux, Jean Louis; Li, Baosheng; Shu, Huazhong

    2015-01-01

    This work presents a novel method for the nonrigid registration of medical images based on the Arimoto entropy, a generalization of the Shannon entropy. The proposed method employed the Jensen–Arimoto divergence measure as a similarity metric to measure the statistical dependence between medical images. Free-form deformations were adopted as the transformation model and the Parzen window estimation was applied to compute the probability distributions. A penalty term is incorporated into the objective function to smooth the nonrigid transformation. The goal of registration is to optimize an objective function consisting of a dissimilarity term and a penalty term, which would be minimal when two deformed images are perfectly aligned using the limited memory BFGS optimization method, and thus to get the optimal geometric transformation. To validate the performance of the proposed method, experiments on both simulated 3D brain MR images and real 3D thoracic CT data sets were designed and performed on the open source elastix package. For the simulated experiments, the registration errors of 3D brain MR images with various magnitudes of known deformations and different levels of noise were measured. For the real data tests, four data sets of 4D thoracic CT from four patients were selected to assess the registration performance of the method, including ten 3D CT images for each 4D CT data covering an entire respiration cycle. These results were compared with the normalized cross correlation and the mutual information methods and show a slight but true improvement in registration accuracy. (paper)

  16. Residual stresses measurement by using ring-core method and 3D digital image correlation technique

    International Nuclear Information System (INIS)

    Hu, Zhenxing; Xie, Huimin; Zhu, Jianguo; Wang, Huaixi; Lu, Jian

    2013-01-01

    Ring-core method/three-dimensional digital image correlation (3D DIC) residual stresses measurement is proposed. Ring-core cutting is a mechanical stress relief method, and combining with 3D DIC system the deformation of the specimen surface can be measured. An optimization iteration method is proposed to obtain the residual stress and rigid-body motion. The method has the ability to cut an annular trench at a different location out of the field of view. A compression test is carried out to demonstrate how residual stress is determined by using 3D DIC system and outfield measurement. The results determined by the approach are in good agreement with the theoretical value. Ring-core/3D DIC has shown its robustness to determine residual stress and can be extended to application in the engineering field. (paper)

  17. Fracture network created by 3D printer and its validation using CT images

    Science.gov (United States)

    Suzuki, A.; Watanabe, N.; Li, K.; Horne, R. N.

    2017-12-01

    Understanding flow mechanisms in fractured media is essential for geoscientific research and geological development industries. This study used 3D printed fracture networks in order to control the properties of fracture distributions inside the sample. The accuracy and appropriateness of creating samples by the 3D printer was investigated by using a X-ray CT scanner. The CT scan images suggest that the 3D printer is able to reproduce complex three-dimensional spatial distributions of fracture networks. Use of hexane after printing was found to be an effective way to remove wax for the post-treatment. Local permeability was obtained by the cubic law and used to calculate the global mean. The experimental value of the permeability was between the arithmetic and geometric means of the numerical results, which is consistent with conventional studies. This methodology based on 3D printed fracture networks can help validate existing flow modeling and numerical methods.

  18. Nanoparticle imaging. 3D structure of individual nanocrystals in solution by electron microscopy.

    Science.gov (United States)

    Park, Jungwon; Elmlund, Hans; Ercius, Peter; Yuk, Jong Min; Limmer, David T; Chen, Qian; Kim, Kwanpyo; Han, Sang Hoon; Weitz, David A; Zettl, A; Alivisatos, A Paul

    2015-07-17

    Knowledge about the synthesis, growth mechanisms, and physical properties of colloidal nanoparticles has been limited by technical impediments. We introduce a method for determining three-dimensional (3D) structures of individual nanoparticles in solution. We combine a graphene liquid cell, high-resolution transmission electron microscopy, a direct electron detector, and an algorithm for single-particle 3D reconstruction originally developed for analysis of biological molecules. This method yielded two 3D structures of individual platinum nanocrystals at near-atomic resolution. Because our method derives the 3D structure from images of individual nanoparticles rotating freely in solution, it enables the analysis of heterogeneous populations of potentially unordered nanoparticles that are synthesized in solution, thereby providing a means to understand the structure and stability of defects at the nanoscale. Copyright © 2015, American Association for the Advancement of Science.

  19. Fracture network created by 3-D printer and its validation using CT images

    Science.gov (United States)

    Suzuki, Anna; Watanabe, Noriaki; Li, Kewen; Horne, Roland N.

    2017-07-01

    Understanding flow mechanisms in fractured media is essential for geoscientific research and geological development industries. This study used 3-D printed fracture networks in order to control the properties of fracture distributions inside the sample. The accuracy and appropriateness of creating samples by the 3-D printer was investigated by using a X-ray CT scanner. The CT scan images suggest that the 3-D printer is able to reproduce complex three-dimensional spatial distributions of fracture networks. Use of hexane after printing was found to be an effective way to remove wax for the posttreatment. Local permeability was obtained by the cubic law and used to calculate the global mean. The experimental value of the permeability was between the arithmetic and geometric means of the numerical results, which is consistent with conventional studies. This methodology based on 3-D printed fracture networks can help validate existing flow modeling and numerical methods.

  20. Computer assisted determination of acetabular cup orientation using 2D-3D image registration

    International Nuclear Information System (INIS)

    Zheng, Guoyan; Zhang, Xuan

    2010-01-01

    2D-3D image-based registration methods have been developed to measure acetabular cup orientation after total hip arthroplasty (THA). These methods require registration of both the prosthesis and the CT images to 2D radiographs and compute implant position with respect to a reference. The application of these methods is limited in clinical practice due to two limitations: (1) the requirement of a computer-aided design (CAD) model of the prosthesis, which may be unavailable due to the proprietary concerns of the manufacturer, and (2) the requirement of either multiple radiographs or radiograph-specific calibration, usually unavailable for retrospective studies. In this paper, we propose a new method to address these limitations. A new formulation for determination of post-operative cup orientation, which couples a radiographic measurement with 2D-3D image matching, was developed. In our formulation, the radiographic measurement can be obtained with known methods so that the challenge lies in the 2D-3D image matching. To solve this problem, a hybrid 2D-3D registration scheme combining a landmark-to-ray 2D-3D alignment with a robust intensity-based 2D-3D registration was used. The hybrid 2D-3D registration scheme allows computing both the post-operative cup orientation with respect to an anatomical reference and the pelvic tilt and rotation with respect to the X-ray imaging table/plate. The method was validated using 2D adult cadaver hips. Using the hybrid 2D-3D registration scheme, our method showed a mean accuracy of 1.0 ± 0.7 (range from 0.1 to 2.0 ) for inclination and 1.7 ± 1.2 (range from 0.0 to 3.9 ) for anteversion, taking the measurements from post-operative CT images as ground truths. Our new solution formulation and the hybrid 2D-3D registration scheme facilitate estimation of post-operative cup orientation and measurement of pelvic tilt and rotation. (orig.)

  1. 3D dynamic pituitary MR imaging with CAIPIRINHA: Initial experience and comparison with 2D dynamic MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Fushimi, Yasutaka, E-mail: yfushimi@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507 (Japan); Okada, Tomohisa; Kanagaki, Mitsunori; Yamamoto, Akira; Kanda, Yumiko; Sakamoto, Ryo [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507 (Japan); Hojo, Masato; Takahashi, Jun C.; Miyamoto, Susumu [Department of Neurosurgery, Kyoto University Graduate School of Medicine, Kyoto 606-8507 (Japan); Togashi, Kaori [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507 (Japan)

    2014-10-15

    Objectives: To evaluate the validity of 3D dynamic pituitary MR imaging with controlled aliasing in parallel imaging results in higher acceleration (CAIPIRINHA), with special emphasis on demarcation of pituitary posterior lobe and stalk. Methods: Participants comprised 32 patients who underwent dynamic pituitary MR imaging due to pituitary or parasellar lesions. 3D dynamic MR with CAIPIRINHA was performed at 3 T with 20-s-interval, precontrast, 1st to 5th dynamic images. Normalized values and enhanced ratios (dynamic postcontrast image values divided by precontrast ones) were compared between 3D and 2D dynamic MR imaging for patients with visual identification of posterior lobe and stalk. Results: In 3D, stalk was identified in 29 patients and unidentified in 3, and posterior lobe was identified in 28 and unidentified in 4. In 2D, stalk was identified in 26 patients and unidentified in 6 patients, and posterior lobe was identified in 15 and unidentified in 17. Normalized values of pituitary posterior lobe and stalk were higher in 3D than 2D (P < 0.001). No significant difference in enhancement ratio was seen between 3D and 2D. Conclusions: 3D dynamic pituitary MR provided better identification and higher normalized values of pituitary posterior lobe and stalk than 2D.

  2. A parallel implementation of 3-d CT image reconstruction on a hypercube multiprocessor

    International Nuclear Information System (INIS)

    Chen, C.M.; Lee, S.Y.; Cho, Z.H.

    1990-01-01

    In this paper, the authors describe how image reconstruction in computerized tomography (CT) can be parallelized on a message-passing multiprocessor. In particular, the results obtained from parallel implementation of 3-D CT image reconstruction for parallel beam geometries on the Intel hypercube, iPSC/2, are presented. A two stage pipelining approach is employed for filtering (convolution) and backprojection. The conventional sequential convolution algorithm is modified such that the symmetry of the filter kernel is fully utilized for parallelization. In the backprojection stage, the 3-D incremental algorithm, the authors' recently developed backprojection scheme which is shown to be faster than conventional algorithm, is parallelized

  3. A GUI visualization system for airborne lidar image data to reconstruct 3D city model

    Science.gov (United States)

    Kawata, Yoshiyuki; Koizumi, Kohei

    2015-10-01

    A visualization toolbox system with graphical user interfaces (GUIs) was developed for the analysis of LiDAR point cloud data, as a compound object oriented widget application in IDL (Interractive Data Language). The main features in our system include file input and output abilities, data conversion capability from ascii formatted LiDAR point cloud data to LiDAR image data whose pixel value corresponds the altitude measured by LiDAR, visualization of 2D/3D images in various processing steps and automatic reconstruction ability of 3D city model. The performance and advantages of our graphical user interface (GUI) visualization system for LiDAR data are demonstrated.

  4. SU-F-I-14: 3D Breast Digital Phantom for XACT Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Tang, S; Laaroussi, R; Chen, J; Samant, P; Xiang, L [University of Oklahoma, Norman, OK (United States); Chen, Y; Ahmad, S [University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States); Yang, K [Massachusetts General Hospital, Boston, MA (United States)

    2016-06-15

    Purpose: The X-ray induced acoustic computed tomography (XACT) is a new imaging modality which combines X-ray contrast and high ultrasonic resolution in a single modality. Using XACT in breast imaging, a 3D breast volume can be imaged by only one pulsed X-ray radiation, which could dramatically reduce the imaging dose for patients undergoing breast cancer screening and diagnosis. A 3D digital phantom that contains both X-ray properties and acoustic properties of different tissue types is indeed needed for developing and optimizing the XACT system. The purpose of this study is to offer a realistic breast digital phantom as a valuable tool for improving breast XACT imaging techniques and potentially leading to better diagnostic outcomes. Methods: A series of breast CT images along the coronal plane from a patient who has breast calcifications are used as the source images. A HU value based segmentation algorithm is employed to identify breast tissues in five categories, namely the skin tissue, fat tissue, glandular tissue, chest bone and calcifications. For each pixel, the dose related parameters, such as material components and density, and acoustic related parameters, such as frequency-dependent acoustic attenuation coefficient and bandwidth, are assigned based on tissue types. Meanwhile, other parameters which are used in sound propagation, including the sound speed, thermal expansion coefficient, and heat capacity are also assigned to each tissue. Results: A series of 2D tissue type image is acquired first and the 3D digital breast phantom is obtained by using commercial 3D reconstruction software. When giving specific settings including dose depositions and ultrasound center frequency, the X-ray induced initial pressure rise can be calculated accordingly. Conclusion: The proposed 3D breast digital phantom represents a realistic breast anatomic structure and provides a valuable tool for developing and evaluating the system performance for XACT.

  5. Web tools for large-scale 3D biological images and atlases

    Directory of Open Access Journals (Sweden)

    Husz Zsolt L

    2012-06-01

    Full Text Available Abstract Background Large-scale volumetric biomedical image data of three or more dimensions are a significant challenge for distributed browsing and visualisation. Many images now exceed 10GB which for most users is too large to handle in terms of computer RAM and network bandwidth. This is aggravated when users need to access tens or hundreds of such images from an archive. Here we solve the problem for 2D section views through archive data delivering compressed tiled images enabling users to browse through very-large volume data in the context of a standard web-browser. The system provides an interactive visualisation for grey-level and colour 3D images including multiple image layers and spatial-data overlay. Results The standard Internet Imaging Protocol (IIP has been extended to enable arbitrary 2D sectioning of 3D data as well a multi-layered images and indexed overlays. The extended protocol is termed IIP3D and we have implemented a matching server to deliver the protocol and a series of Ajax/Javascript client codes that will run in an Internet browser. We have tested the server software on a low-cost linux-based server for image volumes up to 135GB and 64 simultaneous users. The section views are delivered with response times independent of scale and orientation. The exemplar client provided multi-layer image views with user-controlled colour-filtering and overlays. Conclusions Interactive browsing of arbitrary sections through large biomedical-image volumes is made possible by use of an extended internet protocol and efficient server-based image tiling. The tools open the possibility of enabling fast access to large image archives without the requirement of whole image download and client computers with very large memory configurations. The system was demonstrated using a range of medical and biomedical image data extending up to 135GB for a single image volume.

  6. Diagnosis of spinal dural arteriovenous fistula using 3D T2-weighted imaging

    Energy Technology Data Exchange (ETDEWEB)

    Kralik, Stephen F.; Murph, Daniel; Mehta, Peter; O' Neill, Darren P. [Indiana University School of Medicine, Department of Radiology and Imaging Sciences, Indianapolis, IN (United States)

    2017-10-15

    To evaluate spinal MRIs without and with 3D T2W imaging among patients without and with spinal dural arteriovenous fistula (SDAVF) confirmed by spinal digital subtraction angiography (DSA). A retrospective case-control study was performed among patients without and with SDAVF who had both spinal MRIs and gold standard spinal DSA. Two neuroradiologists independently reviewed spinal MRIs that were performed with either sagittal T2W turbo spin echo (2D group) or sagittal 3D T2W sampling perfection with application-optimized contrasts using different flip-angle evolutions (SPACE) (3D group) and documented the presence or absence of SDAVF. Using spinal DSA diagnosis as a gold standard, the sensitivity, specificity, and interobserver agreement for the 2D-group and 3D-group MRI diagnosis were calculated. The 2D group consisted of 21 patients and the 3D group consisted of 16 patients. For both radiologists, the 2D group demonstrated a sensitivity of 100% and specificity of 100%. Interobserver agreement in the 2D group was perfect (k = 1.0). For both radiologists, the 3D group demonstrated sensitivity of 100.0% and specificity of 92.3%. Interobserver agreement in the 3D group was perfect (k = 1.0). While flow voids were considered more conspicuous, spinal cord signal abnormality was considered less conspicuous with 3D T2W SPACE compared with conventional 2D STIR sequence. 3D T2W SPACE should be used in conjunction with 2D T2W sequences to more accurately detect abnormal cord signal and determine when perimedullary flow voids are pathologically abnormal for the radiologic diagnosis of SDAVF. (orig.)

  7. Full-view 3D imaging system for functional and anatomical screening of the breast

    Science.gov (United States)

    Oraevsky, Alexander; Su, Richard; Nguyen, Ha; Moore, James; Lou, Yang; Bhadra, Sayantan; Forte, Luca; Anastasio, Mark; Yang, Wei

    2018-04-01

    Laser Optoacoustic Ultrasonic Imaging System Assembly (LOUISA-3D) was developed in response to demand of diagnostic radiologists for an advanced screening system for the breast to improve on low sensitivity of x-ray based modalities of mammography and tomosynthesis in the dense and heterogeneous breast and low specificity magnetic resonance imaging. It is our working hypothesis that co-registration of quantitatively accurate functional images of the breast vasculature and microvasculature, and anatomical images of breast morphological structures will provide a clinically viable solution for the breast cancer care. Functional imaging is LOUISA-3D is enabled by the full view 3D optoacoustic images acquired at two rapidly toggling laser wavelengths in the near-infrared spectral range. 3D images of the breast anatomical background is enabled in LOUISA-3D by a sequence of B-mode ultrasound slices acquired with a transducer array rotating around the breast. This creates the possibility to visualize distributions of the total hemoglobin and blood oxygen saturation within specific morphological structures such as tumor angiogenesis microvasculature and larger vasculature in proximity of the tumor. The system has four major components: (i) a pulsed dual wavelength laser with fiberoptic light delivery system, (ii) an imaging module with two arc shaped probes (optoacoustic and ultrasonic) placed in a transparent bowl that rotates around the breast, (iii) a multichannel electronic system with analog preamplifiers and digital data acquisition boards, and (iv) computer for the system control, data processing and image reconstruction. The most important advancement of this latest system design compared with previously reported systems is the full breast illumination accomplished for each rotational step of the optoacoustic transducer array using fiberoptic illuminator rotating around the breast independently from rotation of the detector probe. We report here a pilot case studies

  8. 3-D reconstruction of neurons from multichannel confocal laser scanning image series.

    Science.gov (United States)

    Wouterlood, Floris G

    2014-04-10

    A confocal laser scanning microscope (CLSM) collects information from a thin, focal plane and ignores out-of-focus information. Scanning of a specimen, with stepwise axial (Z-) movement of the stage in between each scan, produces Z-series of confocal images of a tissue volume, which then can be used to 3-D reconstruct structures of interest. The operator first configures separate channels (e.g., laser, filters, and detector settings) for each applied fluorochrome and then acquires Z-series of confocal images: one series per channel. Channel signal separation is extremely important. Measures to avoid bleaching are vital. Post-acquisition deconvolution of the image series is often performed to increase resolution before 3-D reconstruction takes place. In the 3-D reconstruction programs described in this unit, reconstructions can be inspected in real time from any viewing angle. By altering viewing angles and by switching channels off and on, the spatial relationships of 3-D-reconstructed structures with respect to structures visualized in other channels can be studied. Since each brand of CLSM, computer program, and 3-D reconstruction package has its own proprietary set of procedures, a general approach is provided in this protocol wherever possible. Copyright © 2014 John Wiley & Sons, Inc.

  9. 3D volume reconstruction from serial breast specimen radiographs for mapping between histology and 3D whole specimen imaging

    NARCIS (Netherlands)

    Mertzanidou, T.; Hipwell, J.H.; Reis, S.; Hawkes, D.J.; Ehteshami Bejnordi, B.; Dalmis, M.U.; Vreemann, S.; Platel, B.; Laak, J.A. van der; Karssemeijer, N.; Hermsen, M.; Bult, P.; Mann, R.M.

    2017-01-01

    PURPOSE: In breast imaging, radiological in vivo images, such as x-ray mammography and magnetic resonance imaging (MRI), are used for tumor detection, diagnosis, and size determination. After excision, the specimen is typically sliced into slabs and a small subset is sampled. Histopathological

  10. Three-dimensional imaging using computer-generated holograms synthesized from 3-D Fourier spectra

    International Nuclear Information System (INIS)

    Yatagai, Toyohiko; Miura, Ken-ichi; Sando, Yusuke; Itoh, Masahide

    2008-01-01

    Computer-generated holograms(CGHs) synthesized from projection images of real existing objects are considered. A series of projection images are recorded both vertically and horizontally with an incoherent light source and a color CCD. According to the principles of computer tomography(CT), the 3-D Fourier spectrum is calculated from several projection images of objects and the Fresnel CGH is synthesized using a part of the 3-D Fourier spectrum. This method has following advantages. At first, no-blur reconstructed images in any direction are obtained owing to two-dimensionally scanning in recording. Secondarily, since not interference fringes but simple projection images of objects are recorded, a coherent light source is not necessary. Moreover, when a color CCD is used in recording, it is easily possible to record and reconstruct colorful objects. Finally, we demonstrate reconstruction of biological objects.

  11. Adaptive fringe-pattern projection for image saturation avoidance in 3D surface-shape measurement.

    Science.gov (United States)

    Li, Dong; Kofman, Jonathan

    2014-04-21

    In fringe-projection 3D surface-shape measurement, image saturation results in incorrect intensities in captured images of fringe patterns, leading to phase and measurement errors. An adaptive fringe-pattern projection (AFPP) method was developed to adapt the maximum input gray level in projected fringe patterns to the local reflectivity of an object surface being measured. The AFPP method demonstrated improved 3D measurement accuracy by avoiding image saturation in highly-reflective surface regions while maintaining high intensity modulation across the entire surface. The AFPP method can avoid image saturation and handle varying surface reflectivity, using only two prior rounds of fringe-pattern projection and image capture to generate the adapted fringe patterns.

  12. Three-dimensional imaging using computer-generated holograms synthesized from 3-D Fourier spectra

    Energy Technology Data Exchange (ETDEWEB)

    Yatagai, Toyohiko; Miura, Ken-ichi; Sando, Yusuke; Itoh, Masahide [University of Tsukba, Institute of Applied Physics, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8571 (Japan)], E-mail: yatagai@cc.utsunomiya-u.ac.jp

    2008-11-01

    Computer-generated holograms(CGHs) synthesized from projection images of real existing objects are considered. A series of projection images are recorded both vertically and horizontally with an incoherent light source and a color CCD. According to the principles of computer tomography(CT), the 3-D Fourier spectrum is calculated from several projection images of objects and the Fresnel CGH is synthesized using a part of the 3-D Fourier spectrum. This method has following advantages. At first, no-blur reconstructed images in any direction are obtained owing to two-dimensionally scanning in recording. Secondarily, since not interference fringes but simple projection images of objects are recorded, a coherent light source is not necessary. Moreover, when a color CCD is used in recording, it is easily possible to record and reconstruct colorful objects. Finally, we demonstrate reconstruction of biological objects.

  13. GPU-Based Block-Wise Nonlocal Means Denoising for 3D Ultrasound Images

    Directory of Open Access Journals (Sweden)

    Liu Li

    2013-01-01

    Full Text Available Speckle suppression plays an important role in improving ultrasound (US image quality. While lots of algorithms have been proposed for 2D US image denoising with remarkable filtering quality, there is relatively less work done on 3D ultrasound speckle suppression, where the whole volume data rather than just one frame needs to be considered. Then, the most crucial problem with 3D US denoising is that the computational complexity increases tremendously. The nonlocal means (NLM provides an effective method for speckle suppression in US images. In this paper, a programmable graphic-processor-unit- (GPU- based fast NLM filter is proposed for 3D ultrasound speckle reduction. A Gamma distribution noise model, which is able to reliably capture image statistics for Log-compressed ultrasound images, was used for the 3D block-wise NLM filter on basis of Bayesian framework. The most significant aspect of our method was the adopting of powerful data-parallel computing capability of GPU to improve the overall efficiency. Experimental results demonstrate that the proposed method can enormously accelerate the algorithm.

  14. 3D conditional generative adversarial networks for high-quality PET image estimation at low dose.

    Science.gov (United States)

    Wang, Yan; Yu, Biting; Wang, Lei; Zu, Chen; Lalush, David S; Lin, Weili; Wu, Xi; Zhou, Jiliu; Shen, Dinggang; Zhou, Luping

    2018-07-01

    Positron emission tomography (PET) is a widely used imaging modality, providing insight into both the biochemical and physiological processes of human body. Usually, a full dose radioactive tracer is required to obtain high-quality PET images for clinical needs. This inevitably raises concerns about potential health hazards. On the other hand, dose reduction may cause the increased noise in the reconstructed PET images, which impacts the image quality to a certain extent. In this paper, in order to reduce the radiation exposure while maintaining the high quality of PET images, we propose a novel method based on 3D conditional generative adversarial networks (3D c-GANs) to estimate the high-quality full-dose PET images from low-dose ones. Generative adversarial networks (GANs) include a generator network and a discriminator network which are trained simultaneously with the goal of one beating the other. Similar to GANs, in the proposed 3D c-GANs, we condition the model on an input low-dose PET image and generate a corresponding output full-dose PET image. Specifically, to render the same underlying information between the low-dose and full-dose PET images, a 3D U-net-like deep architecture which can combine hierarchical features by using skip connection is designed as the generator network to synthesize the full-dose image. In order to guarantee the synthesized PET image to be close to the real one, we take into account of the estimation error loss in addition to the discriminator feedback to train the generator network. Furthermore, a concatenated 3D c-GANs based progressive refinement scheme is also proposed to further improve the quality of estimated images. Validation was done on a real human brain dataset including both the normal subjects and the subjects diagnosed as mild cognitive impairment (MCI). Experimental results show that our proposed 3D c-GANs method outperforms the benchmark methods and achieves much better performance than the state

  15. Knowledge-based analysis and understanding of 3D medical images

    International Nuclear Information System (INIS)

    Dhawan, A.P.; Juvvadi, S.

    1988-01-01

    The anatomical three-dimensional (3D) medical imaging modalities, such as X-ray CT and MRI, have been well recognized in the diagnostic radiology for several years while the nuclear medicine modalities, such as PET, have just started making a strong impact through functional imaging. Though PET images provide the functional information about the human organs, they are hard to interpret because of the lack of anatomical information. The authors objective is to develop a knowledge-based biomedical image analysis system which can interpret the anatomical images (such as CT). The anatomical information thus obtained can then be used in analyzing PET images of the same patient. This will not only help in interpreting PET images but it will also provide a means of studying the correlation between the anatomical and functional imaging. This paper presents the preliminary results of the knowledge based biomedical image analysis system for interpreting CT images of the chest

  16. Minimal Camera Networks for 3D Image Based Modeling of Cultural Heritage Objects

    Science.gov (United States)

    Alsadik, Bashar; Gerke, Markus; Vosselman, George; Daham, Afrah; Jasim, Luma

    2014-01-01

    3D modeling of cultural heritage objects like artifacts, statues and buildings is nowadays an important tool for virtual museums, preservation and restoration. In this paper, we introduce a method to automatically design a minimal imaging network for the 3D modeling of cultural heritage objects. This becomes important for reducing the image capture time and processing when documenting large and complex sites. Moreover, such a minimal camera network design is desirable for imaging non-digitally documented artifacts in museums and other archeological sites to avoid disturbing the visitors for a long time and/or moving delicate precious objects to complete the documentation task. The developed method is tested on the Iraqi famous statue “Lamassu”. Lamassu is a human-headed winged bull of over 4.25 m in height from the era of Ashurnasirpal II (883–859 BC). Close-range photogrammetry is used for the 3D modeling task where a dense ordered imaging network of 45 high resolution images were captured around Lamassu with an object sample distance of 1 mm. These images constitute a dense network and the aim of our study was to apply our method to reduce the number of images for the 3D modeling and at the same time preserve pre-defined point accuracy. Temporary control points were fixed evenly on the body of Lamassu and measured by using a total station for the external validation and scaling purpose. Two network filtering methods are implemented and three different software packages are used to investigate the efficiency of the image orientation and modeling of the statue in the filtered (reduced) image networks. Internal and external validation results prove that minimal image networks can provide highly accurate records and efficiency in terms of visualization, completeness, processing time (>60% reduction) and the final accuracy of 1 mm. PMID:24670718

  17. Minimal camera networks for 3D image based modeling of cultural heritage objects.

    Science.gov (United States)

    Alsadik, Bashar; Gerke, Markus; Vosselman, George; Daham, Afrah; Jasim, Luma

    2014-03-25

    3D modeling of cultural heritage objects like artifacts, statues and buildings is nowadays an important tool for virtual museums, preservation and restoration. In this paper, we introduce a method to automatically design a minimal imaging network for the 3D modeling of cultural heritage objects. This becomes important for reducing the image capture time and processing when documenting large and complex sites. Moreover, such a minimal camera network design is desirable for imaging non-digitally documented artifacts in museums and other archeological sites to avoid disturbing the visitors for a long time and/or moving delicate precious objects to complete the documentation task. The developed method is tested on the Iraqi famous statue "Lamassu". Lamassu is a human-headed winged bull of over 4.25 m in height from the era of Ashurnasirpal II (883-859 BC). Close-range photogrammetry is used for the 3D modeling task where a dense ordered imaging network of 45 high resolution images were captured around Lamassu with an object sample distance of 1 mm. These images constitute a dense network and the aim of our study was to apply our method to reduce the number of images for the 3D modeling and at the same time preserve pre-defined point accuracy. Temporary control points were fixed evenly on the body of Lamassu and measured by using a total station for the external validation and scaling purpose. Two network filtering methods are implemented and three different software packages are used to investigate the efficiency of the image orientation and modeling of the statue in the filtered (reduced) image networks. Internal and external validation results prove that minimal image networks can provide highly accurate records and efficiency in terms of visualization, completeness, processing time (>60% reduction) and the final accuracy of 1 mm.

  18. Automated 3D-Objectdocumentation on the Base of an Image Set

    Directory of Open Access Journals (Sweden)

    Sebastian Vetter

    2011-12-01

    Full Text Available Digital stereo-photogrammetry allows users an automatic evaluation of the spatial dimension and the surface texture of objects. The integration of image analysis techniques simplifies the automation of evaluation of large image sets and offers a high accuracy [1]. Due to the substantial similarities of stereoscopic image pairs, correlation techniques provide measurements of subpixel precision for corresponding image points. With the help of an automated point search algorithm in image sets identical points are used to associate pairs of images to stereo models and group them. The found identical points in all images are basis for calculation of the relative orientation of each stereo model as well as defining the relation of neighboured stereo models. By using proper filter strategies incorrect points are removed and the relative orientation of the stereo model can be made automatically. With the help of 3D-reference points or distances at the object or a defined distance of camera basis the stereo model is orientated absolute. An adapted expansion- and matching algorithm offers the possibility to scan the object surface automatically. The result is a three dimensional point cloud; the scan resolution depends on image quality. With the integration of the iterative closest point- algorithm (ICP these partial point clouds are fitted to a total point cloud. In this way, 3D-reference points are not necessary. With the help of the implemented triangulation algorithm a digital surface models (DSM can be created. The texturing can be made automatically by the usage of the images that were used for scanning the object surface. It is possible to texture the surface model directly or to generate orthophotos automatically. By using of calibrated digital SLR cameras with full frame sensor a high accuracy can be reached. A big advantage is the possibility to control the accuracy and quality of the 3d-objectdocumentation with the resolution of the images. The

  19. Fast isotropic banding-free bSSFP imaging using 3D dynamically phase-cycled radial bSSFP (3D DYPR-SSFP)

    Energy Technology Data Exchange (ETDEWEB)

    Benkert, Thomas; Blaimer, Martin; Breuer, Felix A. [Research Center Magnetic Resonance Bavaria (MRB), Wuerzburg (Germany); Ehses, Philipp [Tuebingen Univ. (Germany). Dept. of Neuroimaging; Max Planck Institute for Biological Cybernetics, Tuebingen (Germany). High-Field MR Center; Jakob, Peter M. [Research Center Magnetic Resonance Bavaria (MRB), Wuerzburg (Germany); Wuerzburg Univ. (Germany). Dept. of Experimental Physics 5

    2016-05-01

    Aims: Dynamically phase-cycled radial balanced steady-state free precession (DYPR-SSFP) is a method for efficient banding artifact removal in bSSFP imaging. Based on a varying radiofrequency (RF) phase-increment in combination with a radial trajectory, DYPR-SSFP allows obtaining a banding-free image out of a single acquired k-space. The purpose of this work is to present an extension of this technique, enabling fast three-dimensional isotropic banding-free bSSFP imaging. Methods: While banding artifact removal with DYPR-SSFP relies on the applied dynamic phase-cycle, this aspect can lead to artifacts, at least when the number of acquired projections lies below a certain limit. However, by using a 3D radial trajectory with quasi-random view ordering for image acquisition, this problem is intrinsically solved, enabling 3D DYPR-SSFP imaging at or even below the Nyquist criterion. The approach is validated for brain and knee imaging at 3 Tesla. Results: Volumetric, banding-free images were obtained in clinically acceptable scan times with an isotropic resolution up to 0.56 mm. Conclusion: The combination of DYPR-SSFP with a 3D radial trajectory allows banding-free isotropic volumetric bSSFP imaging with no expense of scan time. Therefore, this is a promising candidate for clinical applications such as imaging of cranial nerves or articular cartilage.

  20. An external interface for processing 3-D holographic and X-ray images

    International Nuclear Information System (INIS)

    Jueptner, W.; Kreis, T.

    1989-01-01

    The aim of the ESPRIT project 898 is the development of an external interface system, that links physically generated 3-D images to inspection and analysis procedures. While this has to be a general and flexible system, it is used in this project for holographic interferograms and X-ray radiographs for applications in areas such as real time testing and inspection and 3-D measurment. For this task, optical and electronic methods have to be combined in order to extract the relevant information from multiple 3-D images. A further aim of the project is the automation of the holographic interferometry and the X-ray radioscopy for on-line testing in the manufacturing process. (orig./HP)

  1. Near-infrared chemical imaging (NIR-CI) of 3D printed pharmaceuticals

    DEFF Research Database (Denmark)

    Khorasani, Milad; Edinger, Magnus; Raijada, Dharaben Kaushikkumar

    2016-01-01

    Hot-melt extrusion and 3D printing are enabling manufacturing approaches for patient-centred medicinal products. Hot-melt extrusion is a flexible and continuously operating technique which is a crucial part of a typical processing cycle of printed medicines. In this work we use hot-melt extrusion...... for manufacturing of medicinal films containing indomethacin (IND) and polycaprolactone (PCL), extruded strands with nitrofurantoin monohydrate (NFMH) and poly (ethylene oxide) (PEO), and feedstocks for 3D printed dosage forms with nitrofurantoin anhydrate (NFAH), hydroxyapatite (HA) and poly (lactic acid) (PLA......). These feedstocks were printed into a prototype solid dosage form using a desktop 3D printer. These model formulations were characterized using near-infrared chemical imaging (NIR-CI) and, more specifically, the image analytical data were analysed using multivariate curve resolution-alternating least squares (MCR...

  2. An Algorithm for Fast Computation of 3D Zernike Moments for Volumetric Images

    Directory of Open Access Journals (Sweden)

    Khalid M. Hosny

    2012-01-01

    Full Text Available An algorithm was proposed for very fast and low-complexity computation of three-dimensional Zernike moments. The 3D Zernike moments were expressed in terms of exact 3D geometric moments where the later are computed exactly through the mathematical integration of the monomial terms over the digital image/object voxels. A new symmetry-based method was proposed to compute 3D Zernike moments with 87% reduction in the computational complexity. A fast 1D cascade algorithm was also employed to add more complexity reduction. The comparison with existing methods was performed, where the numerical experiments and the complexity analysis ensured the efficiency of the proposed method especially with image and objects of large sizes.

  3. 3D Reconstruction of the Retinal Arterial Tree Using Subject-Specific Fundus Images

    Science.gov (United States)

    Liu, D.; Wood, N. B.; Xu, X. Y.; Witt, N.; Hughes, A. D.; Samcg, Thom

    Systemic diseases, such as hypertension and diabetes, are associated with changes in the retinal microvasculature. Although a number of studies have been performed on the quantitative assessment of the geometrical patterns of the retinal vasculature, previous work has been confined to 2 dimensional (2D) analyses. In this paper, we present an approach to obtain a 3D reconstruction of the retinal arteries from a pair of 2D retinal images acquired in vivo. A simple essential matrix based self-calibration approach was employed for the "fundus camera-eye" system. Vessel segmentation was performed using a semi-automatic approach and correspondence between points from different images was calculated. The results of 3D reconstruction show the centreline of retinal vessels and their 3D curvature clearly. Three-dimensional reconstruction of the retinal vessels is feasible and may be useful in future studies of the retinal vasculature in disease.

  4. A fast time-difference inverse solver for 3D EIT with application to lung imaging.

    Science.gov (United States)

    Javaherian, Ashkan; Soleimani, Manuchehr; Moeller, Knut

    2016-08-01

    A class of sparse optimization techniques that require solely matrix-vector products, rather than an explicit access to the forward matrix and its transpose, has been paid much attention in the recent decade for dealing with large-scale inverse problems. This study tailors application of the so-called Gradient Projection for Sparse Reconstruction (GPSR) to large-scale time-difference three-dimensional electrical impedance tomography (3D EIT). 3D EIT typically suffers from the need for a large number of voxels to cover the whole domain, so its application to real-time imaging, for example monitoring of lung function, remains scarce since the large number of degrees of freedom of the problem extremely increases storage space and reconstruction time. This study shows the great potential of the GPSR for large-size time-difference 3D EIT. Further studies are needed to improve its accuracy for imaging small-size anomalies.

  5. Inter-plane artifact suppression in tomosynthesis using 3D CT image data

    Directory of Open Access Journals (Sweden)

    Kim Jae G

    2011-12-01

    Full Text Available Abstract Background Despite its superb lateral resolution, flat-panel-detector (FPD based tomosynthesis suffers from low contrast and inter-plane artifacts caused by incomplete cancellation of the projection components stemming from outside the focal plane. The incomplete cancellation of the projection components, mostly due to the limited scan angle in the conventional tomosynthesis scan geometry, often makes the image contrast too low to differentiate the malignant tissues from the background tissues with confidence. Methods In this paper, we propose a new method to suppress the inter-plane artifacts in FPD-based tomosynthesis. If 3D whole volume CT images are available before the tomosynthesis scan, the CT image data can be incorporated into the tomosynthesis image reconstruction to suppress the inter-plane artifacts, hence, improving the image contrast. In the proposed technique, the projection components stemming from outside the region-of-interest (ROI are subtracted from the measured tomosynthesis projection data to suppress the inter-plane artifacts. The projection components stemming from outside the ROI are calculated from the 3D whole volume CT images which usually have lower lateral resolution than the tomosynthesis images. The tomosynthesis images are reconstructed from the subtracted projection data which account for the x-ray attenuation through the ROI. After verifying the proposed method by simulation, we have performed both CT scan and tomosynthesis scan on a phantom and a sacrificed rat using a FPD-based micro-CT. Results We have measured contrast-to-noise ratio (CNR from the tomosynthesis images which is an indicator of the residual inter-plane artifacts on the focal-plane image. In both cases of the simulation and experimental imaging studies of the contrast evaluating phantom, CNRs have been significantly improved by the proposed method. In the rat imaging also, we have observed better visual contrast from the tomosynthesis

  6. Inter-plane artifact suppression in tomosynthesis using 3D CT image data

    Science.gov (United States)

    2011-01-01

    Background Despite its superb lateral resolution, flat-panel-detector (FPD) based tomosynthesis suffers from low contrast and inter-plane artifacts caused by incomplete cancellation of the projection components stemming from outside the focal plane. The incomplete cancellation of the projection components, mostly due to the limited scan angle in the conventional tomosynthesis scan geometry, often makes the image contrast too low to differentiate the malignant tissues from the background tissues with confidence. Methods In this paper, we propose a new method to suppress the inter-plane artifacts in FPD-based tomosynthesis. If 3D whole volume CT images are available before the tomosynthesis scan, the CT image data can be incorporated into the tomosynthesis image reconstruction to suppress the inter-plane artifacts, hence, improving the image contrast. In the proposed technique, the projection components stemming from outside the region-of-interest (ROI) are subtracted from the measured tomosynthesis projection data to suppress the inter-plane artifacts. The projection components stemming from outside the ROI are calculated from the 3D whole volume CT images which usually have lower lateral resolution than the tomosynthesis images. The tomosynthesis images are reconstructed from the subtracted projection data which account for the x-ray attenuation through the ROI. After verifying the proposed method by simulation, we have performed both CT scan and tomosynthesis scan on a phantom and a sacrificed rat using a FPD-based micro-CT. Results We have measured contrast-to-noise ratio (CNR) from the tomosynthesis images which is an indicator of the residual inter-plane artifacts on the focal-plane image. In both cases of the simulation and experimental imaging studies of the contrast evaluating phantom, CNRs have been significantly improved by the proposed method. In the rat imaging also, we have observed better visual contrast from the tomosynthesis images reconstructed by

  7. High-resolution 3D X-ray imaging of intracranial nitinol stents

    International Nuclear Information System (INIS)

    Snoeren, Rudolph M.; With, Peter H.N. de; Soederman, Michael; Kroon, Johannes N.; Roijers, Ruben B.; Babic, Drazenko

    2012-01-01

    To assess an optimized 3D imaging protocol for intracranial nitinol stents in 3D C-arm flat detector imaging. For this purpose, an image quality simulation and an in vitro study was carried out. Nitinol stents of various brands were placed inside an anthropomorphic head phantom, using iodine contrast. Experiments with objects were preceded by image quality and dose simulations. We varied X-ray imaging parameters in a commercially interventional X-ray system to set 3D image quality in the contrast-noise-sharpness space. Beam quality was varied to evaluate contrast of the stents while keeping absorbed dose below recommended values. Two detector formats were used, paired with an appropriate pixel size and X-ray focus size. Zoomed reconstructions were carried out and snapshot images acquired. High contrast spatial resolution was assessed with a CT phantom. We found an optimal protocol for imaging intracranial nitinol stents. Contrast resolution was optimized for nickel-titanium-containing stents. A high spatial resolution larger than 2.1 lp/mm allows struts to be visualized. We obtained images of stents of various brands and a representative set of images is shown. Independent of the make, struts can be imaged with virtually continuous strokes. Measured absorbed doses are shown to be lower than 50 mGy Computed Tomography Dose Index (CTDI). By balancing the modulation transfer of the imaging components and tuning the high-contrast imaging capabilities, we have shown that thin nitinol stent wires can be reconstructed with high contrast-to-noise ratio and good detail, while keeping radiation doses within recommended values. Experimental results compare well with imaging simulations. (orig.)

  8. From morphology to clinical pathophysiology: multiphoton fluorescence lifetime imaging at patients' bedside

    Science.gov (United States)

    Mess, Christian; Zens, Katharina; Gorzelanny, Christian; Metze, Dieter; Luger, Thomas A.; König, Karsten; Schneider, Stefan W.; Huck, Volker

    2017-02-01

    Application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of skin diseases. By means of multiphoton excitation, endogenous biomolecules like NADH, collagen or elastin show autofluorescence or second harmonic generation. Thus, these molecules provide information about the subcellular morphology, epidermal architecture and physiological condition of the skin. To gain a deeper understanding of the linkage between cellular structure and physiological processes, non-invasive multiphotonbased intravital tomography (MPT) and fluorescence lifetime imaging (FLIM) were combined within the scopes of inflammatory skin, chronic wounds and drug delivery in clinical application. The optical biopsies generated via MPT were morphologically analyzed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Independent morphometric algorithms reliably showed a perinuclear accumulation in lesional skin in contrast to an even distribution in healthy skin. Confirmatively, MPT-FLIM showed an obvious metabolic shift in lesions. Moreover, detection of the onset and progression of inflammatory processes could be achieved. The feasibility of primary in vivo tracking of applied therapeutic agents further broadened our scope: We examined the permeation and subsequent distribution of agents directly visualized in patientś skin in short-term repetitive measurements. Furthermore, we performed MPT-FLIM follow-up investigations in the long-term course of therapy. Therefore, clinical MPT-FLIM application offers new insights into the pathophysiology and the individual therapeutic course of skin diseases, facilitating a better understanding of the processes of inflammation and wound healing.

  9. Imaging of the 3D dynamics of flagellar beating in human sperm.

    Science.gov (United States)

    Silva-Villalobos, F; Pimentel, J A; Darszon, A; Corkidi, G

    2014-01-01

    The study of the mechanical and environmental factors that regulate a fundamental event such as fertilization have been subject of multiple studies. Nevertheless, the microscopical size of the spermatozoa and the high beating frequency of their flagella (up to 20 Hz) impose a series of technological challenges for the study of the mechanical factors implicated. Traditionally, due to the inherent characteristics of the rapid sperm movement, and to the technological limitations of microscopes (optical or confocal) to follow in three dimensions (3D) their movement, the analysis of their dynamics has been studied in two dimensions, when the head is confined to a surface. Flagella propel sperm and while their head can be confined to a surface, flagellar movement is not restricted to 2D, always displaying 3D components. In this work, we present a highly novel and useful tool to analyze sperm flagella dynamics in 3D. The basis of the method is a 100 Hz oscillating objective mounted on a bright field optical microscope covering a 16 microns depth space at a rate of ~ 5000 images per second. The best flagellum focused subregions were associated to their respective Z real 3D position. Unprecedented graphical results making evident the 3D movement of the flagella are shown in this work and supplemental material illustrating a 3D animation using the obtained experimental results is also included.

  10. Comparison of 3D magnetic resonance imaging and digital subtraction angiography for intracranial artery stenosis

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ji Eun; Jung, Seung Chai; Kim, Ho Sung; Choi, Choong-Gon; Kim, Sang Joon; Lee, Deok Hee [University of Ulsan College of Medicine, Department of Radiology and Research Institute of Radiology, Asan Medical Center, Seoul (Korea, Republic of); Lee, Sang Hun; Kwon, Sun U.; Kang, Dong-Wha; Kim, Jong S. [University of Ulsan College of Medicine, Department of Neurology, Ulsan (Korea, Republic of); Jeon, Ji Young [Gachon University, Department of Radiology, Gil Medical Center, Incheon (Korea, Republic of); Lee, Ji Ye [Soonchunhyang University Bucheon Hospital, Department of Radiology, Bucheon (Korea, Republic of); Kim, Seon-Ok [University of Ulsan College of Medicine, Department of Clinical Epidemiology and Biostatistics, Asan Medical Center, Seoul (Korea, Republic of)

    2017-11-15

    To compare three-dimensional high-resolution magnetic resonance imaging (3D HR-MRI) and digital subtraction angiography (DSA) for diagnosing and evaluating stenosis in the entire circle of Willis. The study included 516 intracranial arteries from 43 patients with intracranial artery stenosis (ICAS) who underwent both 3D HR-MRI and DSA within 1 month. Two readers independently diagnosed atherosclerosis, dissection, moyamoya disease and vasculitis, rated their diagnostic confidence for each vessel and measured the luminal diameters. Reference standard was made from clinico-radiologic diagnosis. Diagnostic accuracy, diagnostic confidence, the degree of stenosis and luminal diameter were assessed and compared between both modalities. For atherosclerosis, 3D HR-MRI showed better diagnostic accuracy (P =.03-.003), sensitivity (P =.006-.01) and positive predictive value (P ≤.001-.006) compared to DSA. Overall, the readers were more confident of their diagnosis of ICAS when using 3D HR-MRI (reader 1, P ≤.001-.007; reader 2, P ≤.001-.015). 3D HR-MRI showed similar degree of stenosis (P >.05) and higher luminal diameter (P <.05) compared to DSA. 3D HR-MRI might be useful to evaluate atherosclerosis, with better diagnostic confidence and comparable stenosis measurement compared to DSA in the entire circle of Willis. (orig.)

  11. Accelerated 3D-OSEM image reconstruction using a Beowulf PC cluster for pinhole SPECT

    International Nuclear Information System (INIS)

    Zeniya, Tsutomu; Watabe, Hiroshi; Sohlberg, Antti; Iida, Hidehiro

    2007-01-01

    A conventional pinhole single-photon emission computed tomography (SPECT) with a single circular orbit has limitations associated with non-uniform spatial resolution or axial blurring. Recently, we demonstrated that three-dimensional (3D) images with uniform spatial resolution and no blurring can be obtained by complete data acquired using two-circular orbit, combined with the 3D ordered subsets expectation maximization (OSEM) reconstruction method. However, a long computation time is required to obtain the reconstruction image, because of the fact that 3D-OSEM is an iterative method and two-orbit acquisition doubles the size of the projection data. To reduce the long reconstruction time, we parallelized the two-orbit pinhole 3D-OSEM reconstruction process by using a Beowulf personal computer (PC) cluster. The Beowulf PC cluster consists of seven PCs connected to Gbit Ethernet switches. Message passing interface protocol was utilized for parallelizing the reconstruction process. The projection data in a subset are distributed to each PC. The partial image forward-and back-projected in each PC is transferred to all PCs. The current image estimate on each PC is updated after summing the partial images. The performance of parallelization on the PC cluster was evaluated using two independent projection data sets acquired by a pinhole SPECT system with two different circular orbits. Parallelization using the PC cluster improved the reconstruction time with increasing number of PCs. The reconstruction time of 54 min by the single PC was decreased to 10 min when six or seven PCs were used. The speed-up factor was 5.4. The reconstruction image by the PC cluster was virtually identical with that by the single PC. Parallelization of 3D-OSEM reconstruction for pinhole SPECT using the PC cluster can significantly reduce the computation time, whereas its implementation is simple and inexpensive. (author)

  12. Applications of 3-D reconstruction and 3-D image analysis using computer graphics in surgery of the oral and maxillofacial regions

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Koichi; Yamauchi, Asanori; Madachi, Fujio; Furuta, Isao

    1988-12-01

    Using the 2-D data provided by CT-Tomography and MRI-tomography of oral and maxillofacial diseases (cyst, benign tumor, primary tumor and regional lymphnodes of malignant tumor), 3-D images were reconstructed and spatial analysis was attempted. We report the general concepts. The hardware used consisted of the Hewlett-Packard HP-9000/300, which utilizes a 16-bit CPU. A digitizer was used to construct 3-D images from serial CT-tomography and MRI-tomography images. Output was displayed on a color monitor and photographs. The 3 cases on which we used this technique included a 19-year-old male with plunging ranula, a 50-year-old male with maxillary pleomorphic adenoma, and a 58-year-old male with squamous cell carcinoma of the maxillary sinus (T/sub 3/N/sub 3/M/sub 0/). As 3-D reconstruction can be done in any arbitrary direction or cross section, it is possible to spatially determine the position of the disease inside the body, its progression, and its relationship with adjacent organs. Through image analysis, it is possible to better understand the volume and surface area of the disease. 3-D image reconstruction is an effective tool in the determination of diagnosis, therapeutic guidelines, and surgical indications, as well as effectiveness of treatment.

  13. Applications of 3-D reconstruction and 3-D image analysis using computer graphics in surgery of the oral and maxillofacial regions

    International Nuclear Information System (INIS)

    Yamamoto, Koichi; Yamauchi, Asanori; Madachi, Fujio; Furuta, Isao

    1988-01-01

    Using the 2-D data provided by CT-Tomography and MRI-tomography of oral and maxillofacial diseases (cyst, benign tumor, primary tumor and regional lymphnodes of malignant tumor), 3-D images were reconstructed and spatial analysis was attempted. We report the general concepts. The hardware used consisted of the Hewlett-Packard HP-9000/300, which utilizes a 16-bit CPU. A digitizer was used to construct 3-D images from serial CT-tomography and MRI-tomography images. Output was displayed on a color monitor and photographs. The 3 cases on which we used this technique included a 19-year-old male with plunging ranula, a 50-year-old male with maxillary pleomorphic adenoma, and a 58-year-old male with squamous cell carcinoma of the maxillary sinus (T 3 N 3 M 0 ). As 3-D reconstruction can be done in any arbitrary direction or cross section, it is possible to spatially determine the position of the disease inside the body, its progression, and its relationship with adjacent organs. Through image analysis, it is possible to better understand the volume and surface area of the disease. 3-D image reconstruction is an effective tool in the determination of diagnosis, therapeutic guidelines, and surgical indications, as well as effectiveness of treatment. (author)

  14. 3-D display of magnetic resonance images by use of multiplex holography

    International Nuclear Information System (INIS)

    Oshita, Hiroshi; Yokoi, Shigeki; Toriwaki, Jun-ichiro; Matsuo, Michimasa.

    1987-01-01

    In this paper, we study the method of generating a true 3-D image from MRI multiple slices by using the multiplex holography. The purpose in our method is to display effectively the density information distributed in the 3-D space. For making a multiplex hologram any projected image in each direction from multiple slices should be computed. We study computer processing for producing images of good quality from the viewpoint of displaying the density distribution in the 3-D space clearly and discriminating lesions from normal tissues. The following two kinds of processing are studied. (1) Projection: To generate fastly projections of good quality, the following subjects are examined. 1. Computation method of density values on a projected image from an original slice image. 2. Methods for projection of multiple slices. 3. Interpolation of slices between original neighbouring slices. 4. Composition of several sets of multiple slices in different directions. (2) Image enahancement: To enhance depth feeling of slices and density values in lesions, the following methods are studied. 1. Enhancement of depth feeling by a weighted sum of slices. 2. Slice enhancement by multiplying the particular slices by weights. 3. Lesion enhancement by thresholding. 4. Lesion enhancement by operations between images with different imaging parameters. The following are the results ; projected images of the quality good enough for constructing a multiplex hologram can be obtained by the simple method proposed in this paper, using four sets of multiple slices in four different directions. The enhancement technique mentioned above was proved to be effective for improving the understandability of 3-D information. (author)

  15. Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

    Science.gov (United States)

    Chen, Youting; Chen, Jing; Chen, Hong; Hong, Zhipeng; Zhu, Xiaoqin; Zhuo, Shuangmu; Chen, Yanling; Chen, Jianxin

    2014-09-01

    Hematoxylin and eosin (H&E) staining of tissue samples is the standard approach in histopathology for imaging and diagnosing cancer. Recent reports have shown that multiphoton microscopy (MPM) provides better sample interface with single-cell resolution, which enhances traditional H&E staining and offers a powerful diagnostic tool with potential applications in oncology. The purpose of this study was to further expand the versatility of MPM by establishing the optical parameters required for imaging unstained histological sections of pancreatic neoplasms, thereby providing an efficient and environmentally sustainable alternative to H&E staining while improving the accuracy of pancreatic cancer diagnoses. We found that the high-resolution MPM images clearly distinguish between the structure of normal pancreatic tissues compared with pancreatic neoplasms in unstained histological sections, and discernable differences in tissue architecture and cell morphology between normal versus tumorigenic cells led to enhanced optical diagnosis of cancerous tissue. Moreover, quantitative assessment of the cytomorphological features visualized from MPM images showed significant differences in the nuclear-cytoplasmic ratios of pancreatic neoplasms compared with normal pancreas, as well as further distinguished pancreatic malignant tumors from benign tumors. These results indicate that the MPM could potentially serve as an optical tool for the diagnosis of pancreatic neoplasms in unstained histological sections.

  16. 2D sparse array transducer optimization for 3D ultrasound imaging

    International Nuclear Information System (INIS)

    Choi, Jae Hoon; Park, Kwan Kyu

    2014-01-01

    A 3D ultrasound image is desired in many medical examinations. However, the implementation of a 2D array, which is needed for a 3D image, is challenging with respect to fabrication, interconnection and cabling. A 2D sparse array, which needs fewer elements than a dense array, is a realistic way to achieve 3D images. Because the number of ways the elements can be placed in an array is extremely large, a method for optimizing the array configuration is needed. Previous research placed the target point far from the transducer array, making it impossible to optimize the array in the operating range. In our study, we focused on optimizing a 2D sparse array transducer for 3D imaging by using a simulated annealing method. We compared the far-field optimization method with the near-field optimization method by analyzing a point-spread function (PSF). The resolution of the optimized sparse array is comparable to that of the dense array.

  17. 3D imaging by serial block face scanning electron microscopy for materials science using ultramicrotomy.

    Science.gov (United States)

    Hashimoto, Teruo; Thompson, George E; Zhou, Xiaorong; Withers, Philip J

    2016-04-01

    Mechanical serial block face scanning electron microscopy (SBFSEM) has emerged as a means of obtaining three dimensional (3D) electron images over volumes much larger than possible by focused ion beam (FIB) serial sectioning and at higher spatial resolution than achievable with conventional X-ray computed tomography (CT). Such high resolution 3D electron images can be employed for precisely determining the shape, volume fraction, distribution and connectivity of important microstructural features. While soft (fixed or frozen) biological samples are particularly well suited for nanoscale sectioning using an ultramicrotome, the technique can also produce excellent 3D images at electron microscope resolution in a time and resource-efficient manner for engineering materials. Currently, a lack of appreciation of the capabilities of ultramicrotomy and the operational challenges associated with minimising artefacts for different materials is limiting its wider application to engineering materials. Consequently, this paper outlines the current state of the art for SBFSEM examining in detail how damage is introduced during slicing and highlighting strategies for minimising such damage. A particular focus of the study is the acquisition of 3D images for a variety of metallic and coated systems. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  18. Estimating 3D Object Parameters from 2D Grey-Level Images

    NARCIS (Netherlands)

    Houkes, Z.

    2000-01-01

    This thesis describes a general framework for parameter estimation, which is suitable for computer vision applications. The approach described combines 3D modelling, animation and estimation tools to determine parameters of objects in a scene from 2D grey-level images. The animation tool predicts

  19. High-accuracy and real-time 3D positioning, tracking system for medical imaging applications based on 3D digital image correlation

    Science.gov (United States)

    Xue, Yuan; Cheng, Teng; Xu, Xiaohai; Gao, Zeren; Li, Qianqian; Liu, Xiaojing; Wang, Xing; Song, Rui; Ju, Xiangyang; Zhang, Qingchuan

    2017-01-01

    This paper presents a system for positioning markers and tracking the pose of a rigid object with 6 degrees of freedom in real-time using 3D digital image correlation, with two examples for medical imaging applications. Traditional DIC method was improved to meet the requirements of the real-time by simplifying the computations of integral pixel search. Experiments were carried out and the results indicated that the new method improved the computational efficiency by about 4-10 times in comparison with the traditional DIC method. The system was aimed for orthognathic surgery navigation in order to track the maxilla segment after LeFort I osteotomy. Experiments showed noise for the static point was at the level of 10-3 mm and the measurement accuracy was 0.009 mm. The system was demonstrated on skin surface shape evaluation of a hand for finger stretching exercises, which indicated a great potential on tracking muscle and skin movements.

  20. Accurate localization of intracavitary brachytherapy applicators from 3D CT imaging studies

    International Nuclear Information System (INIS)

    Lerma, F.A.; Williamson, J.F.

    2002-01-01

    Purpose: To present an accurate method to identify the positions and orientations of intracavitary (ICT) brachytherapy applicators imaged in 3D CT scans, in support of Monte Carlo photon-transport simulations, enabling accurate dose modeling in the presence of applicator shielding and interapplicator attenuation. Materials and methods: The method consists of finding the transformation that maximizes the coincidence between the known 3D shapes of each applicator component (colpostats and tandem) with the volume defined by contours of the corresponding surface on each CT slice. We use this technique to localize Fletcher-Suit CT-compatible applicators for three cervix cancer patients using post-implant CT examinations (3 mm slice thickness and separation). Dose distributions in 1-to-1 registration with the underlying CT anatomy are derived from 3D Monte Carlo photon-transport simulations incorporating each applicator's internal geometry (source encapsulation, high-density shields, and applicator body) oriented in relation to the dose matrix according to the measured localization transformations. The precision and accuracy of our localization method are assessed using CT scans, in which the positions and orientations of dense rods and spheres (in a precision-machined phantom) were measured at various orientations relative to the gantry. Results: Using this method, we register 3D Monte Carlo dose calculations directly onto post insertion patient CT studies. Using CT studies of a precisely machined phantom, the absolute accuracy of the method was found to be ±0.2 mm in plane, and ±0.3 mm in the axial direction while its precision was ±0.2 mm in plane, and ±0.2 mm axially. Conclusion: We have developed a novel, and accurate technique to localize intracavitary brachytherapy applicators in 3D CT imaging studies, which supports 3D dose planning involving detailed 3D Monte Carlo dose calculations, modeling source positions, shielding and interapplicator shielding

  1. Lensfree diffractive tomography for the imaging of 3D cell cultures

    Science.gov (United States)

    Berdeu, Anthony; Momey, Fabien; Dinten, Jean-Marc; Gidrol, Xavier; Picollet-D'hahan, Nathalie; Allier, Cédric

    2017-02-01

    New microscopes are needed to help reaching the full potential of 3D organoid culture studies by gathering large quantitative and systematic data over extended periods of time while preserving the integrity of the living sample. In order to reconstruct large volumes while preserving the ability to catch every single cell, we propose new imaging platforms based on lens-free microscopy, a technic which is addressing these needs in the context of 2D cell culture, providing label-free and non-phototoxic acquisition of large datasets. We built lens-free diffractive tomography setups performing multi-angle acquisitions of 3D organoid cultures embedded in Matrigel and developed dedicated 3D holographic reconstruction algorithms based on the Fourier diffraction theorem. Nonetheless, holographic setups do not record the phase of the incident wave front and the biological samples in Petri dish strongly limit the angular coverage. These limitations introduce numerous artefacts in the sample reconstruction. We developed several methods to overcome them, such as multi-wavelength imaging or iterative phase retrieval. The most promising technic currently developed is based on a regularised inverse problem approach directly applied on the 3D volume to reconstruct. 3D reconstructions were performed on several complex samples such as 3D networks or spheroids embedded in capsules with large reconstructed volumes up to 25 mm3 while still being able to identify single cells. To our knowledge, this is the first time that such an inverse problem approach is implemented in the context of lens-free diffractive tomography enabling to reconstruct large fully 3D volumes of unstained biological samples.

  2. An Open Source Low-Cost Automatic System for Image-Based 3d Digitization

    Science.gov (United States)

    Menna, F.; Nocerino, E.; Morabito, D.; Farella, E. M.; Perini, M.; Remondino, F.

    2017-11-01

    3D digitization of heritage artefacts, reverse engineering of industrial components or rapid prototyping-driven design are key topics today. Indeed, millions of archaeological finds all over the world need to be surveyed in 3D either to allow convenient investigations by researchers or because they are inaccessible to visitors and scientists or, unfortunately, because they are seriously endangered by wars and terrorist attacks. On the other hand, in case of industrial and design components there is often the need of deformation analyses or physical replicas starting from reality-based 3D digitisations. The paper is aligned with these needs and presents the realization of the ORION (arduinO Raspberry pI rOtating table for image based 3D recostructioN) prototype system, with its hardware and software components, providing critical insights about its modular design. ORION is an image-based 3D reconstruction system based on automated photogrammetric acquisitions and processing. The system is being developed under a collaborative educational project between FBK Trento, the University of Trento and internship programs with high school in the Trentino province (Italy).

  3. Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging.

    Directory of Open Access Journals (Sweden)

    Lina Carlini

    Full Text Available Three-dimensional (3D localization-based super-resolution microscopy (SR requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, which we term `wobble`, results in warped 3D SR images and provide a software tool to correct this distortion. This system-specific, lateral shift is typically > 80 nm across an axial range of ~ 1 μm. A theoretical analysis based on phase retrieval data from our microscope suggests that the wobble is caused by non-rotationally symmetric phase and amplitude aberrations in the microscope's pupil function. We then apply our correction to the bacterial cytoskeletal protein FtsZ in live bacteria and demonstrate that the corrected data more accurately represent the true shape of this vertically-oriented ring-like structure. We also include this correction method in a registration procedure for dual-color, 3D SR data and show that it improves target registration error (TRE at the axial limits over an imaging depth of 1 μm, yielding TRE values of < 20 nm. This work highlights the importance of correcting aberrations in 3D SR to achieve high fidelity between the measurements and the sample.

  4. AN OPEN SOURCE LOW-COST AUTOMATIC SYSTEM FOR IMAGE-BASED 3D DIGITIZATION

    Directory of Open Access Journals (Sweden)

    F. Menna

    2017-11-01

    Full Text Available 3D digitization of heritage artefacts, reverse engineering of industrial components or rapid prototyping-driven design are key topics today. Indeed, millions of archaeological finds all over the world need to be surveyed in 3D either to allow convenient investigations by researchers or because they are inaccessible to visitors and scientists or, unfortunately, because they are seriously endangered by wars and terrorist attacks. On the other hand, in case of industrial and design components there is often the need of deformation analyses or physical replicas starting from reality-based 3D digitisations. The paper is aligned with these needs and presents the realization of the ORION (arduinO Raspberry pI rOtating table for image based 3D recostructioN prototype system, with its hardware and software components, providing critical insights about its modular design. ORION is an image-based 3D reconstruction system based on automated photogrammetric acquisitions and processing. The system is being developed under a collaborative educational project between FBK Trento, the University of Trento and internship programs with high school in the Trentino province (Italy.

  5. Multiphoton processes

    International Nuclear Information System (INIS)

    Manus, C.; Mainfray, G.

    1980-01-01

    The main features of multiphoton processes are described on a somewhat elementary basis. The emphasis is put on multiphoton ionization of atoms where the influence of resonance effects is given through typical examples. The important role played by the coherence of light is shown to produce a very dramatic influence on multiphoton absorption. Different observations concerning molecules, electrons, as well as solid surfaces illustrate the generality of these very non linear interaction between light and matter

  6. Quantitative roughness characterization and 3D reconstruction of electrode surface using cyclic voltammetry and SEM image

    Energy Technology Data Exchange (ETDEWEB)

    Dhillon, Shweta; Kant, Rama, E-mail: rkant@chemistry.du.ac.in

    2013-10-01

    Area measurements from cyclic voltammetry (CV) and image from scanning electron microscopy (SEM) are used to characterize electrode statistical morphology, 3D surface reconstruction and its electroactivity. SEM images of single phased materials correspond to two-dimensional (2D) projections of 3D structures, leading to an incomplete characterization. Lack of third dimension information in SEM image is circumvented using equivalence between denoised SEM image and CV area measurements. This CV-SEM method can be used to estimate power spectral density (PSD), width, gradient, finite fractal nature of roughness and local morphology of the electrode. We show that the surface morphological statistical property like distribution function of gradient can be related to local electro-activity. Electrode surface gradient micrographs generated here can provide map of electro-activity sites. Finally, the densely and uniformly packed small gradient over the Pt-surface is the determining criterion for high intrinsic electrode activity.

  7. Review of 3D image data calibration for heterogeneity correction in proton therapy treatment planning

    International Nuclear Information System (INIS)

    Zhu, Jiahua; Penfold, Scott N.

    2016-01-01

    Correct modelling of the interaction parameters of patient tissues is of vital importance in proton therapy treatment planning because of the large dose gradients associated with the Bragg peak. Different 3D imaging techniques yield different information regarding these interaction parameters. Given the rapidly expanding interest in proton therapy, this review is written to make readers aware of the current challenges in accounting for tissue heterogeneities and the imaging systems that are proposed to tackle these challenges. A summary of the interaction parameters of interest in proton therapy and the current and developmental 3D imaging techniques used in proton therapy treatment planning is given. The different methods to translate the imaging data to the interaction parameters of interest are reviewed and a summary of the implementations in several commercial treatment planning systems is presented.

  8. Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy

    Directory of Open Access Journals (Sweden)

    Emilio J Gualda

    2014-08-01

    Full Text Available The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three dimensional matrix, highly resembling physiological conditions. Light sheet fluorescence microscopy is becoming an excellent tool for fast imaging of such three-dimensional biological structures. We demonstrate the potential of this technique for the imaging of human differentiated 3D neural aggregates in fixed and live samples, namely calcium imaging and cell death processes, showing the power of imaging modality compared with traditional microscopy. The combination of light sheet microscopy and 3D neural cultures will open the door to more challenging experiments involving drug testing at large scale as well as a better understanding of relevant biological processes in a more realistic environment.

  9. Review of 3D image data calibration for heterogeneity correction in proton therapy treatment planning.

    Science.gov (United States)

    Zhu, Jiahua; Penfold, Scott N

    2016-06-01

    Correct modelling of the interaction parameters of patient tissues is of vital importance in proton therapy treatment planning because of the large dose gradients associated with the Bragg peak. Different 3D imaging techniques yield different information regarding these interaction parameters. Given the rapidly expanding interest in proton therapy, this review is written to make readers aware of the current challenges in accounting for tissue heterogeneities and the imaging systems that are proposed to tackle these challenges. A summary of the interaction parameters of interest in proton therapy and the current and developmental 3D imaging techniques used in proton therapy treatment planning is given. The different methods to translate the imaging data to the interaction parameters of interest are reviewed and a summary of the implementations in several commercial treatment planning systems is presented.

  10. Quantitative roughness characterization and 3D reconstruction of electrode surface using cyclic voltammetry and SEM image

    International Nuclear Information System (INIS)

    Dhillon, Shweta; Kant, Rama

    2013-01-01

    Area measurements from cyclic voltammetry (CV) and image from scanning electron microscopy (SEM) are used to characterize electrode statistical morphology, 3D surface reconstruction and its electroactivity. SEM images of single phased materials correspond to two-dimensional (2D) projections of 3D structures, leading to an incomplete characterization. Lack of third dimension information in SEM image is circumvented using equivalence between denoised SEM image and CV area measurements. This CV-SEM method can be used to estimate power spectral density (PSD), width, gradient, finite fractal nature of roughness and local morphology of the electrode. We show that the surface morphological statistical property like distribution function of gradient can be related to local electro-activity. Electrode surface gradient micrographs generated here can provide map of electro-activity sites. Finally, the densely and uniformly packed small gradient over the Pt-surface is the determining criterion for high intrinsic electrode activity.

  11. VERIFICATION OF 3D BUILDING MODELS USING MUTUAL INFORMATION IN AIRBORNE OBLIQUE IMAGES

    Directory of Open Access Journals (Sweden)

    A. P. Nyaruhuma

    2012-07-01

    Full Text Available This paper describes a method for automatic verification of 3D building models using airborne oblique images. The problem being tackled is identifying buildings that are demolished or changed since the models were constructed or identifying wrong models using the images. The models verified are of CityGML LOD2 or higher since their edges are expected to coincide with actual building edges. The verification approach is based on information theory. Corresponding variables between building models and oblique images are used for deriving mutual information for individual edges, faces or whole buildings, and combined for all perspective images available for the building. The wireframe model edges are projected to images and verified using low level image features – the image pixel gradient directions. A building part is only checked against images in which it may be visible. The method has been tested with models constructed using laser points against Pictometry images that are available for most cities of Europe and may be publically viewed in the so called Birds Eye view of the Microsoft Bing Maps. Results are that nearly all buildings are correctly categorised as existing or demolished. Because we now concentrate only on roofs we also used the method to test and compare results from nadir images. This comparison made clear that especially height errors in models can be more reliably detected in oblique images because of the tilted view. Besides overall building verification, results per individual edges can be used for improving the 3D building models.

  12. Filters in 2D and 3D Cardiac SPECT Image Processing

    Directory of Open Access Journals (Sweden)

    Maria Lyra

    2014-01-01

    Full Text Available Nuclear cardiac imaging is a noninvasive, sensitive method providing information on cardiac structure and physiology. Single photon emission tomography (SPECT evaluates myocardial perfusion, viability, and function and is widely used in clinical routine. The quality of the tomographic image is a key for accurate diagnosis. Image filtering, a mathematical processing, compensates for loss of detail in an image while reducing image noise, and it can improve the image resolution and limit the degradation of the image. SPECT images are then reconstructed, either by filter back projection (FBP analytical technique or iteratively, by algebraic methods. The aim of this study is to review filters in cardiac 2D, 3D, and 4D SPECT applications and how these affect the image quality mirroring the diagnostic accuracy of SPECT images. Several filters, including the Hanning, Butterworth, and Parzen filters, were evaluated in combination with the two reconstruction methods as well as with a specified MatLab program. Results showed that for both 3D and 4D cardiac SPECT the Butterworth filter, for different critical frequencies and orders, produced the best results. Between the two reconstruction methods, the iterative one might be more appropriate for cardiac SPECT, since it improves lesion detectability due to the significant improvement of image contrast.

  13. Physics-based optimization of image quality in 3D X-ray flat-panel cone-beam imaging

    NARCIS (Netherlands)

    Snoeren, R.M.

    2012-01-01

    This thesis describes the techniques for modeling and control of 3D X-ray cardiovascular systems in terms of Image Quality and patient dose, aiming at optimizing the diagnostic quality. When aiming at maximum Image Quality (IQ), a cascaded system constituted from inter-dependent imaging components,

  14. Evaluation of 3D printing materials for fabrication of a novel multi-functional 3D thyroid phantom for medical dosimetry and image quality

    Science.gov (United States)

    Alssabbagh, Moayyad; Tajuddin, Abd Aziz; Abdulmanap, Mahayuddin; Zainon, Rafidah

    2017-06-01

    Recently, the three-dimensional printer has started to be utilized strongly in medical industries. In the human body, many parts or organs can be printed from 3D images to meet accurate organ geometries. In this study, five common 3D printing materials were evaluated in terms of their elementary composition and the mass attenuation coefficients. The online version of XCOM photon cross-section database was used to obtain the attenuation values of each material. The results were compared with the attenuation values of the thyroid listed in the International Commission on Radiation Units and Measurements - ICRU 44. Two original thyroid models (hollow-inside and solid-inside) were designed from scratch to be used in nuclear medicine, diagnostic radiology and radiotherapy for dosimetry and image quality purposes. Both designs have three holes for installation of radiation dosimeters. The hollow-inside model has more two holes in the top for injection the radioactive materials. The attenuation properties of the Polylactic Acid (PLA) material showed a very good match with the thyroid tissue, which it was selected to 3D print the phantom using open source RepRap, Prusa i3 3D printer. The scintigraphy images show that the phantom simulates a real healthy thyroid gland and thus it can be used for image quality purposes. The measured CT numbers of the PA material after the 3D printing show a close match with the human thyroid CT numbers. Furthermore, the phantom shows a good accommodation of the TLD dosimeters inside the holes. The 3D fabricated thyroid phantom simulates the real shape of the human thyroid gland with a changeable geometrical shape-size feature to fit different age groups. By using 3D printing technology, the time required to fabricate the 3D phantom was considerably shortened compared to the longer conventional methods, where it took only 30 min to print out the model. The 3D printing material used in this study is commercially available and cost

  15. The value of the 3D CT imaging in diagnosis of lumbar spondylolysis

    International Nuclear Information System (INIS)

    Krupski, W.; Paslawski, M.; Zlomaniec, J.; Fatyga, M.; Majcher, P.

    2003-01-01

    The frequent cause of a low back pain is the lumbar spondylolysis and the spondylolisthesis. The purpose of the study was to assess of the value of three-dimensional CT imaging in diagnosis of the lumbar spondylolysis. Material comprises of 22 patients complaining from low back pain in which lateral radiograms, axial CT scans, MPR and 3D reconstructions were performed. The presence of spondylolysis, spondylolisthesis, stenosis of the spinal canal and intervertebral foramens were assessed. The differences in diagnostic value between analysed imaging modalities in revealing spondylolysis, spondylolisthesis and narrowing of intervertebral foramens, were statistically highly significant. The highest sensitivity in recognition of these pathologies was observed in 3D reconstruction. The 3D reconstructions were also useful in an assessment of the spinal canal stenosis, revealing degenerative changes, but the increased number of diagnosed pathologies was not statistically significant comparing with axial CT section. Spondylolysis was diagnosed in 22 patients based on 3D reconstructions, in 14 patients on MPR reconstructions, in 18 patients on axial sections and only in 8 cases on lateral radiograms. Spondylolisthesis was visible on lateral radiograms in 21 patients, on axial scans in 12 patients, and in 22 cases, on both MPR and 3D reconstruction. The stenosis of the spinal canal was found on lateral radiograms in 2 patients, on MPR reconstruction in 4 cases, and in 7 patients on 3D reconstruction. The intervertebral foramen stenosis was present in 5 patients, based on MPR reconstruction and in 17, on spatial images. Spatial 3D CT reconstructions are superior to lateral radiograms, axial CT sections and MPR reconstruction in revealing spondylolysis, spondylolisthesis and stenosis of intervertebral foramens. They are useful in assessment of spinal canal narrowing and evaluation of degenerative changes. In our opinion 3D CT reconstruction projected from the inside of the

  16. Automatic detection of patient identification and positioning errors in radiotherapy treatment using 3D setup images

    OpenAIRE

    Jani, Shyam

    2015-01-01

    The success of modern radiotherapy treatment depends on the correct alignment of the radiation beams with the target region in the patient. In the conventional paradigm of image-guided radiation therapy, 2D or 3D setup images are taken immediately prior to treatment and are used by radiation therapy technologists to localize the patient to the same position as defined from the reference planning CT dataset. However, numerous reports in the literature have described errors during this step, wh...

  17. Accurate 3D kinematic measurement of temporomandibular joint using X-ray fluoroscopic images

    Science.gov (United States)

    Yamazaki, Takaharu; Matsumoto, Akiko; Sugamoto, Kazuomi; Matsumoto, Ken; Kakimoto, Naoya; Yura, Yoshiaki

    2014-04-01

    Accurate measurement and analysis of 3D kinematics of temporomandibular joint (TMJ) is very important for assisting clinical diagnosis and treatment of prosthodontics and orthodontics, and oral surgery. This study presents a new 3D kinematic measurement technique of the TMJ using X-ray fluoroscopic images, which can easily obtain the TMJ kinematic data in natural motion. In vivo kinematics of the TMJ (maxilla and mandibular bone) is determined using a feature-based 2D/3D registration, which uses beads silhouette on fluoroscopic images and 3D surface bone models with beads. The 3D surface models of maxilla and mandibular bone with beads were created from CT scans data of the subject using the mouthpiece with the seven strategically placed beads. In order to validate the accuracy of pose estimation for the maxilla and mandibular bone, computer simulation test was performed using five patterns of synthetic tantalum beads silhouette images. In the clinical applications, dynamic movement during jaw opening and closing was conducted, and the relative pose of the mandibular bone with respect to the maxilla bone was determined. The results of computer simulation test showed that the root mean square errors were sufficiently smaller than 1.0 mm and 1.0 degree. In the results of clinical application, during jaw opening from 0.0 to 36.8 degree of rotation, mandibular condyle exhibited 19.8 mm of anterior sliding relative to maxillary articular fossa, and these measurement values were clinically similar to the previous reports. Consequently, present technique was thought to be suitable for the 3D TMJ kinematic analysis.

  18. Fast imaging of laboratory core floods using 3D compressed sensing RARE MRI.

    Science.gov (United States)

    Ramskill, N P; Bush, I; Sederman, A J; Mantle, M D; Benning, M; Anger, B C; Appel, M; Gladden, L F

    2016-09-01

    Three-dimensional (3D) imaging of the fluid distributions within the rock is essential to enable the unambiguous interpretation of core flooding data. Magnetic resonance imaging (MRI) has been widely used to image fluid saturation in rock cores; however, conventional acquisition strategies are typically too slow to capture the dynamic nature of the displacement processes that are of interest. Using Compressed Sensing (CS), it is possible to reconstruct a near-perfect image from significantly fewer measurements than was previously thought necessary, and this can result in a significant reduction in the image acquisition times. In the present study, a method using the Rapid Acquisition with Relaxation Enhancement (RARE) pulse sequence with CS to provide 3D images of the fluid saturation in rock core samples during laboratory core floods is demonstrated. An objective method using image quality metrics for the determination of the most suitable regularisation functional to be used in the CS reconstructions is reported. It is shown that for the present application, Total Variation outperforms the Haar and Daubechies3 wavelet families in terms of the agreement of their respective CS reconstructions with a fully-sampled reference image. Using the CS-RARE approach, 3D images of the fluid saturation in the rock core have been acquired in 16min. The CS-RARE technique has been applied to image the residual water saturation in the rock during a water-water displacement core flood. With a flow rate corresponding to an interstitial velocity of vi=1.89±0.03ftday(-1), 0.1 pore volumes were injected over the course of each image acquisition, a four-fold reduction when compared to a fully-sampled RARE acquisition. Finally, the 3D CS-RARE technique has been used to image the drainage of dodecane into the water-saturated rock in which the dynamics of the coalescence of discrete clusters of the non-wetting phase are clearly observed. The enhancement in the temporal resolution that has

  19. Interactive visualization and analysis of 3D medical images for neurosurgery

    International Nuclear Information System (INIS)

    Miyazawa, Tatsuo; Otsuki, Taisuke.

    1994-01-01

    We propose a method that makes it possible to interactively rotate and zoom a volume-rendered object and to interactively manipulate a function for transferring data values to color and opacity. The method ray-traces a Value-Intensity-Strength volume (VIS volume) instead of a color-opacity volume, and uses an adaptive refinement technique in generating images. The VIS volume tracing method can reduce by 20-90 percent the computational time of re-calculation necessitated by changing the function for transferring data values to color and opacity, and can reduce the computational time of pre-processing by 20 percent. It can also reduce the required memory space by 40 percent. The combination of VIS volume tracing and adaptive refinement method makes it possible to interactively visualize and analyze 3D medical image data. Once we can see detailed image of 3D objects to determine their orientation, we can easily manipulate the viewing and rendering parameters even while viewing rough, blurred images. The increase in the computation time for generating full-resolution images by using the adaptive refinement technique is approximately five to ten percent. Its effectiveness is evaluated by using the results of visualization for some 3D medical image data. (author)

  20. High-resolution 3D laser imaging based on tunable fiber array link

    Science.gov (United States)

    Zhao, Sisi; Ruan, Ningjuan; Yang, Song

    2017-10-01

    Airborne photoelectric reconnaissance system with the bore sight down to the ground is an important battlefield situational awareness system, which can be used for reconnaissance and surveillance of complex ground scene. Airborne 3D imaging Lidar system is recognized as the most potential candidates for target detection under the complex background, and is progressing in the directions of high resolution, long distance detection, high sensitivity, low power consumption, high reliability, eye safe and multi-functional. However, the traditional 3D laser imaging system has the disadvantages of lower imaging resolutions because of the small size of the existing detector, and large volume. This paper proposes a high resolution laser 3D imaging technology based on the tunable optical fiber array link. The echo signal is modulated by a tunable optical fiber array link and then transmitted to the focal plane detector. The detector converts the optical signal into electrical signals which is given to the computer. Then, the computer accomplishes the signal calculation and image restoration based on modulation information, and then reconstructs the target image. This paper establishes the mathematical model of tunable optical fiber array signal receiving link, and proposes the simulation and analysis of the affect factors on high density multidimensional point cloud reconstruction.

  1. Automated segmentation and geometrical modeling of the tricuspid aortic valve in 3D echocardiographic images.

    Science.gov (United States)

    Pouch, Alison M; Wang, Hongzhi; Takabe, Manabu; Jackson, Benjamin M; Sehgal, Chandra M; Gorman, Joseph H; Gorman, Robert C; Yushkevich, Paul A

    2013-01-01

    The aortic valve has been described with variable anatomical definitions, and the consistency of 2D manual measurement of valve dimensions in medical image data has been questionable. Given the importance of image-based morphological assessment in the diagnosis and surgical treatment of aortic valve disease, there is considerable need to develop a standardized framework for 3D valve segmentation and shape representation. Towards this goal, this work integrates template-based medial modeling and multi-atlas label fusion techniques to automatically delineate and quantitatively describe aortic leaflet geometry in 3D echocardiographic (3DE) images, a challenging task that has been explored only to a limited extent. The method makes use of expert knowledge of aortic leaflet image appearance, generates segmentations with consistent topology, and establishes a shape-based coordinate system on the aortic leaflets that enables standardized automated measurements. In this study, the algorithm is evaluated on 11 3DE images of normal human aortic leaflets acquired at mid systole. The clinical relevance of the method is its ability to capture leaflet geometry in 3DE image data with minimal user interaction while producing consistent measurements of 3D aortic leaflet geometry.

  2. Multiphoton microscopy for the in-situ investigation of cellular processes and integrity in cryopreservation.

    Science.gov (United States)

    Doerr, Daniel; Stark, Martin; Ehrhart, Friederike; Zimmermann, Heiko; Stracke, Frank

    2009-08-01

    In this study we demonstrate a new noninvasive imaging method to monitor freezing processes in biological samples and to investigate life in the frozen state. It combines a laser scanning microscope with a computer-controlled cryostage. Nearinfrared (NIR) femtosecond laser pulses evoke the fluorescence of endogenous fluorophores and fluorescent labels due to multiphoton absorption.The inherent optical nonlinearity of multiphoton absorption allows 3D fluorescence imaging for optical tomography of frozen biological material in-situ. As an example for functional imaging we use fluorescence lifetime imaging (FLIM) to create images with chemical and physical contrast.

  3. Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo

    Science.gov (United States)

    Dancik, Yuri; Favre, Amandine; Loy, Chong Jin; Zvyagin, Andrei V.; Roberts, Michael S.

    2013-02-01

    There is a growing body of literature showing the usefulness of multiphoton tomography (MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and the ensuing development of noninvasive diagnostic tools against skin diseases. Melanin and pigmentation-associated skin cancers constitute some of the major applications. We show that MPT and fluorescence lifetime imaging can be used to measure changes in cutaneous melanin concentration and that these can be related to the visible skin color. Melanin in the skin of African, Indian, Caucasian, and Asian volunteers is detected on the basis of its emission wavelength and fluorescence lifetimes in solution and in a melanocyte-keratinocyte cell culture. Fluorescence intensity is used to characterize the melanin content and distribution as a function of skin type and depth into the skin (stratum granulosum and stratum basale). The measured fluorescence intensities in given skin types agree with melanin amounts reported by others using biopsies. Our results suggest that spatial distribution of melanin in skin can be studied using MPT and fluorescence lifetime imaging, but further studies are needed to ascertain that the method can resolve melanin amount in smaller depth intervals.

  4. 3D-imaging of the knee with an optimized 3D-FSE-sequence and a 15-channel knee-coil

    Energy Technology Data Exchange (ETDEWEB)

    Notohamiprodjo, Mike, E-mail: mike.notohamiprodjo@med.uni-muenchen.de [Department of Clinical Radiology, University Hospitals Munich, Marchioninistrasse 15, 81377 Munich (Germany); Department of Radiology, Langone Medical Center, Bernard and Irene Schwartz Center for Biomedical Imaging New York University, 660 First Avenue, 4th Floor, New York, NY 10016 (United States); Horng, Annie; Kuschel, Bernhard [Department of Clinical Radiology, University Hospitals Munich, Marchioninistrasse 15, 81377 Munich (Germany); Paul, Dominik [Siemens Healthcare, Erlangen, Henkestr. 127, 91054 Erlangen (Germany); Li, Guobin [Siemens Mindit Magnetic Resonance Ltd., Shenzhen, Guang Dong (China); Raya, Jose G. [Department of Radiology, Langone Medical Center, Bernard and Irene Schwartz Center for Biomedical Imaging New York University, 660 First Avenue, 4th Floor, New York, NY 10016 (United States); Reiser, Maximilian F. [Department of Clinical Radiology, University Hospitals Munich, Marchioninistrasse 15, 81377 Munich (Germany); Glaser, Christian [Department of Clinical Radiology, University Hospitals Munich, Marchioninistrasse 15, 81377 Munich (Germany); Department of Radiology, Langone Medical Center, Bernard and Irene Schwartz Center for Biomedical Imaging New York University, 660 First Avenue, 4th Floor, New York, NY 10016 (United States)

    2012-11-15

    Objectives: To evaluate the clinical usefulness of an optimized 3D-Fast-Spin-Echo-sequence (3D-SPACE) in combination with a 15-channel knee-coil for 3D-imaging of the knee at 3 T. Methods: 15 volunteers and 50 consecutive patients were examined at 3 T with fat-saturated moderately T2-weighted 3D-SPACE (Voxel-size (VS): 0.6 mm Multiplication-Sign 0.5 mm Multiplication-Sign 0.5 mm/acquisition-time (AT) 10:44 min) using a 15-channel knee-coil. Flip angle optimization and radial k-space reordering were applied. Signal- and contrast-to-noise-ratios (SNR, CNR) were compared to non-optimized 3D-SPACE (8-channel knee-coil) and conventional 2D-FSE (VS: 0.4 mm Multiplication-Sign 0.4 mm Multiplication-Sign 3 mm/total AT: 12 min). Two radiologists independently rated depiction of internal knee structures and assessed detection and depiction of cartilage and meniscus abnormalities compared to conventional 2D-FSE-sequences. Sensitivity and specificity were calculated for a subgroup with arthroscopy as reference standard. Statistical analysis was performed with paired t-tests, confidence intervals and weighted-{kappa}-coefficients. Results: SNR and CNR particularly of fluid/cartilage of optimized 3D-SPACE were significantly higher (p < 0.05) than of the non-optimized 3D-sequence and conventional 2D-sequence. Blurring and image inhomogeneity were reduced in the optimized sequence. The thin slice-thickness was beneficial for depiction of problematical anatomical structures such as meniscal roots. 3D-SPACE showed significantly higher diagnostic confidence (p < 0.05) for diagnosis of cartilage lesions of the femoral trochlea. Overall sensitivity and specificity of 3D-SPACE and 2D-FSE for cartilage lesions was 82.3%/80.2% and 79.4%/84.2% and 100%/86.4% and 92.3%/81.8% for meniscus lesions. Conclusions: Optimized 3D-SPACE provides significantly higher signal and contrast compared to conventional 2D-FSE, particularly for fluid and cartilage, leading to improved diagnostic confidence

  5. Dual optimization based prostate zonal segmentation in 3D MR images.

    Science.gov (United States)

    Qiu, Wu; Yuan, Jing; Ukwatta, Eranga; Sun, Yue; Rajchl, Martin; Fenster, Aaron

    2014-05-01

    Efficient and accurate segmentation of the prostate and two of its clinically meaningful sub-regions: the central gland (CG) and peripheral zone (PZ), from 3D MR images, is of great interest in image-guided prostate interventions and diagnosis of prostate cancer. In this work, a novel multi-region segmentation approach is proposed to simultaneously segment the prostate and its two major sub-regions from only a single 3D T2-weighted (T2w) MR image, which makes use of the prior spatial region consistency and incorporates a customized prostate appearance model into the segmentation task. The formulated challenging combinatorial optimization problem is solved by means of convex relaxation, for which a novel spatially continuous max-flow model is introduced as the dual optimization formulation to the studied convex relaxed optimization problem with region consistency constraints. The proposed continuous max-flow model derives an efficient duality-based algorithm that enjoys numerical advantages and can be easily implemented on GPUs. The proposed approach was validated using 18 3D prostate T2w MR images with a body-coil and 25 images with an endo-rectal coil. Experimental results demonstrate that the proposed method is capable of efficiently and accurately extracting both the prostate zones: CG and PZ, and the whole prostate gland from the input 3D prostate MR images, with a mean Dice similarity coefficient (DSC) of 89.3±3.2% for the whole gland (WG), 82.2±3.0% for the CG, and 69.1±6.9% for the PZ in 3D body-coil MR images; 89.2±3.3% for the WG, 83.0±2.4% for the CG, and 70.0±6.5% for the PZ in 3D endo-rectal coil MR images. In addition, the experiments of intra- and inter-observer variability introduced by user initialization indicate a good reproducibility of the proposed approach in terms of volume difference (VD) and coefficient-of-variation (CV) of DSC. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. 3D BUILDING RECONSTRUCTION BY MULTIVIEW IMAGES AND THE INTEGRATED APPLICATION WITH AUGMENTED REALITY

    Directory of Open Access Journals (Sweden)

    J.-T. Hwang

    2016-10-01

    Full Text Available This study presents an approach wherein photographs with a high degree of overlap are clicked using a digital camera and used to generate three-dimensional (3D point clouds via feature point extraction and matching. To reconstruct a building model, an unmanned aerial vehicle (UAV is used to click photographs from vertical shooting angles above the building. Multiview images are taken from the ground to eliminate the shielding effect on UAV images caused by trees. Point clouds from the UAV and multiview images are generated via Pix4Dmapper. By merging two sets of point clouds via tie points, the complete building model is reconstructed. The 3D models are reconstructed using AutoCAD 2016 to generate vectors from the point clouds; SketchUp Make 2016 is used to rebuild a complete building model with textures. To apply 3D building models in urban planning and design, a modern approach is to rebuild the digital models; however, replacing the landscape design and building distribution in real time is difficult as the frequency of building replacement increases. One potential solution to these problems is augmented reality (AR. Using Unity3D and Vuforia to design and implement the smartphone application service, a markerless AR of the building model can be built. This study is aimed at providing technical and design skills related to urban planning, urban designing, and building information retrieval using AR.

  7. Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity.

    Science.gov (United States)

    Lee, Karen J I; Calder, Grant M; Hindle, Christopher R; Newman, Jacob L; Robinson, Simon N; Avondo, Jerome J H Y; Coen, Enrico S

    2017-01-01

    Optical projection tomography (OPT) is a well-established method for visualising gene activity in plants and animals. However, a limitation of conventional OPT is that the specimen upper size limit precludes its application to larger structures. To address this problem we constructed a macro version called Macro OPT (M-OPT). We apply M-OPT to 3D live imaging of gene activity in growing whole plants and to visualise structural morphology in large optically cleared plant and insect specimens up to 60 mm tall and 45 mm deep. We also show how M-OPT can be used to image gene expression domains in 3D within fixed tissue and to visualise gene activity in 3D in clones of growing young whole Arabidopsis plants. A further application of M-OPT is to visualise plant-insect interactions. Thus M-OPT provides an effective 3D imaging platform that allows the study of gene activity, internal plant structures and plant-insect interactions at a macroscopic scale. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  8. Identification of hydrodynamic forces around 3D surrogates using particle image velocimetry in a microfluidic channel

    Science.gov (United States)

    Afshar, Sepideh; Nath, Shubhankar; Demirci, Utkan; Hasan, Tayyaba; Scarcelli, Giuliano; Rizvi, Imran; Franco, Walfre

    2018-02-01

    Previous studies have demonstrated that flow-induced shear stress induces a motile and aggressive tumor phenotype in a microfluidic model of 3D ovarian cancer. However, the magnitude and distribution of the hydrodynamic forces that influence this biological modulation on the 3D cancer nodules are not known. We have developed a series of numerical and experimental tools to identify these forces within a 3D microchannel. In this work, we used particle image velocimetry (PIV) to find the velocity profile using fluorescent micro-spheres as surrogates and nano-particles as tracers, from which hydrodynamic forces can be derived. The fluid velocity is obtained by imaging the trajectory of a range of florescence nano-particles (500-800 μm) via confocal microscopy. Imaging was done at different horizontal planes and with a 50 μm bead as the surrogate. For an inlet current rate of 2 μl/s, the maximum velocity at the center of the channel was 51 μm/s. The velocity profile around the sphere was symmetric which is expected since the flow is dominated by viscous forces as opposed to inertial forces. The confocal PIV was successfully employed in finding the velocity profile in a microchannel with a nodule surrogate; therefore, it seems feasible to use PIV to investigate the hydrodynamic forces around 3D biological models.

  9. An efficient dictionary learning algorithm and its application to 3-D medical image denoising.

    Science.gov (United States)

    Li, Shutao; Fang, Leyuan; Yin, Haitao

    2012-02-01

    In this pa